title: Modulation of Paecilomyces lilacinus antimicrobial metabolite production by co-culturing with Salmonella typhimurium.
abstract: Fungi are important organisms related to the production of antibiotics, many of which are commercially available and used in the clinic to treat infections. Infectious diseases represent a leading cause of death worldwide, being a severe threat to public health, especially with the rise of multi-resistant microorganisms. Therefore, the search for new antibiotic substances is regaining importance. The aim of this study was to evaluate the modulation of the antimicrobial activity of Paecilomyces lilacinus extracts obtained after fungal cultivation in challenging growing conditions, which included the co-cultivation with Salmonella typhimurium, in different levels of inactivation. Two methodologies were used to achieve the antimicrobial response levels of the extracts: disc diffusion and broth microdilution. The extracts showed selective antibacterial activity in response to the presence of bacterial DNA in the medium. The extracts were analysed by HPLC that corroborated that parameter variation influenced the extract chemical profiles.
authors.affiliation: Escola de Farmácia, Universidade Federal de Ouro Preto, Ouro Preto, MG 35400-000, Brazil.
authors.forename: Ana Paula C
authors.initials: AP
authors.lastname: Teles
authors.forename: Gabriela S
authors.initials: GS
authors.lastname: Ataliba
authors.forename: Jacqueline A
authors.initials: JA
authors.lastname: Takahashi
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D004269:DNA, Bacterial; D010143:Paecilomyces / Q000737:chemistry*; D012486:Salmonella typhimurium / Q000187:drug effects*
chemical_list: D000890:Anti-Infective Agents; D004269:DNA, Bacterial