title: An evaluation of the effects of nonselective and cardioselective β-blockers on wound healing in Sprague Dawley rats.
abstract: OBJECTIVE
  To investigate the effect of a nonselective β-blocker (propranolol) and cardioselective β-blocker (metoprolol) on wound healing in rats using incision and excision wound models and to compare the effect of these drugs on wound healing.
  
  
  METHODS
  Propranolol and metoprolol were given orally. Sprague Dawley rats of either sex were used. Incision and excision wound models were used to evaluate the wound-healing activity. Effects of metoprolol and propranolol on tensile strength, period of epithelialization, and hydroxyproline content were observed. Histological analysis was done to see collagen deposition and inflammatory infiltrate.
  
  
  METHODS
  The data was subjected to analysis of variance (ANOVA) followed by Scheffe's test. P < 0.05 was considered to be statistically significant. Statistical analysis was done using SPSS software version 15.0.
  
  
  RESULTS
  Administration of propranolol or metoprolol was shown to decrease tensile strength, delay wound contraction and re-epithelialization, increase inflammatory infiltrate, and reduce collagen density and hydroxyproline levels.
  
  
  CONCLUSIONS
  The results suggest that nonselective and cardioselective β-blockers delay wound healing and these effects are mediated by β1-receptors.
authors.affiliation: Department of Pharmacology, L.T.M. Medical College and General Hospital, Sion, Mumbai, India.
authors.forename: Sanket B
authors.initials: SB
authors.lastname: Raut
authors.forename: Sharmada R
authors.initials: SR
authors.lastname: Nerlekar
authors.forename: Sudhir
authors.initials: S
authors.lastname: Pawar
authors.forename: Amol N
authors.initials: AN
authors.lastname: Patil
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005260:Female; D008297:Male; D008790:Metoprolol / Q000494:pharmacology*; D011433:Propranolol / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D000319:Adrenergic beta-Antagonists; D002316:Cardiotonic Agents; D011433:Propranolol; D008790:Metoprolol
keywords: Excision wound model; incision wound model; metoprolol; propranolol