title: Genomic diversity among group A rotaviruses from diarrheic children, piglets, buffalo and cow calves of Madhya Pradesh.
abstract: Diarrheal disease continues to be a global health problem, particularly among young ones in developing nations. Amongst several viral and non-viral agents associated with diarrhea, group A rotavirus has been recognized as the major etiological agent of childhood gastroenteritis in human infants as well as several animal species throughout the world. During this study, a total of 181 diarrheic stool samples collected from children, piglets, buffalo and cow calves of Madhya Pradesh, central India were analyzed by electrophoretic mobilities of the 11 segments of dsRNA by polyacrylamide gel electrophoresis (PAGE). This technique revealed prevalence of rotavirus among different species (human-26.09%, pig-25.71%, buffalo-23.61% and cattle-21.43%). Prevalence of existence of circulating 8 different electropherotypes of group A rotaviruses indicated high genomic diversity among rotaviruses in this geographical region. Majority of the electropherotypes from humans and animals were of long pattern (75%) than short electropherotypes (9.09%). Same electropherotype was found to exist either only in a single species or in more than one species implicating the possibility of cross species transmission of the rotavirus strains. As it was found that certain animal rotavirus strains had electropherotypic similarities to some human strains, speculation increased about whether animals play a role as a source of rotavirus infection in humans or vice-versa. There is a need for further detailed study on the molecular characterization of rotaviruses which would have important implication in vaccine evaluation program.
authors.affiliation: College of Veterinary Science and A. H., Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, 482001 MP India.
authors.forename: Anupam Lal
authors.initials: AL
authors.lastname: Kusumakar
authors.lastname: Savita
authors.forename: Yashpal Singh
authors.initials: YS
authors.lastname: Malik
authors.lastname: Minakshi
authors.forename: Gaya
authors.initials: G
authors.lastname: Prasad
keywords: Diarrheic infants; Genomic diversity; Group A rotaviruses; RNA-electropherotypes