title: Distinct strains of Leishmania major induce different cytokine mRNA expression in draining lymph node of BALB/c mice.
abstract: Four genotypically distinct strains of L. major collected from persons residing in different endemic areas of cutaneous leishmaniasis in Iran were evaluated in BALB/c mice. Parasite virulence was evaluated by measuring the parasite burden in the lymph nodes. Immunogenicity of the strains was assessed by analysis of cytokines mRNA expression levels in popliteal lymph nodes of the mice in early (3, 16, 40 h) and late (week 1, W3, W5 and W8) time periods after infection. The expression of cytokines mRNA, namely Ifng, Il2,Il4,Il10 and Il12, was quantitated by real-time PCR. The lowest and the highest parasite loads were induced by Damghan (2·15 × 10⁷) and Shiraz (9·59 × 10⁹) strains, respectively. Moreover, Damghan strain elicited higher expression levels of Ifng and Il2 mRNA and the highest ratio of Ifng/Il4 mRNA expression compared with the other strains at 40 h and 8 weeks post-infection. The results indicate that the inoculation of BALB/c mice with different strains induced high diversity in parasite burden and cytokines gene expression. Amongst the four strains, Damghan strain showed the lowest parasite load and the highest tendency to induce expression of Th1 cytokines gene and might be considered as a safe and immunogenic strain.
authors.affiliation: Immunology Department, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Asadpour
authors.forename: F
authors.initials: F
authors.lastname: Riazi-Rad
authors.forename: V
authors.initials: V
authors.lastname: Khaze
authors.forename: S
authors.initials: S
authors.lastname: Ajdary
authors.forename: M H
authors.initials: MH
authors.lastname: Alimohammadian
mesh_terms: D000818:Animals; D000917:Antibody Formation; D016207:Cytokines / Q000235:genetics* / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation; D007371:Interferon-gamma; D018664:Interleukin-12 / Q000235:genetics / Q000276:immunology; D015847:Interleukin-4 / Q000235:genetics / Q000276:immunology; D018320:Leishmania major / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology* / Q000469:parasitology; D008198:Lymph Nodes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D059208:Parasite Load; D014774:Virulence
chemical_list: D016207:Cytokines; D018664:Interleukin-12; D015847:Interleukin-4; D007371:Interferon-gamma