title: UbcH10 overexpression in human lung carcinomas and its correlation with EGFR and p53 mutational status.
abstract: BACKGROUND
  UbcH10 codes for the cancer related E2 Ubiquitin Conjugating Enzyme, an enzymatic molecule with a key role in the ubiquitin-proteasome pathway. Current studies have suggested a critical role of UbcH10 in a variety of malignancies, including human thyroid, breast, ovarian and colorectal carcinomas. The aim of this study has been to extend the analysis of UbcH10 expression to lung cancer. This neoplasia represents one of the leading cause of cancer mortality worldwide, and new tools for an accurate diagnosis/prognosis are needed.
  
  
  METHODS
  The expression levels of UbcH10 were analysed in human non-small cell lung carcinoma (NSCLC) by quantitative RT-PCR and tissue microarray immunohistochemistry, and these values were correlated with the clinicopathological features of the patients affected by NSCLC.
  
  
  RESULTS
  Our results demonstrate that UbcH10 is overexpressed in NSCLC compared to the normal lung tissue. Moreover, UbcH10 expression is significantly higher in squamous cell and large cell carcinomas than in adenocarcinomas, and directly and inversely correlated with the mutational status of p53 and EGFR, respectively. The suppression of UbcH10 expression by RNAi resulted in a drastic reduction of proliferation and migration abilities of lung carcinoma cell lines.
  
  
  CONCLUSIONS
  These results, taken together, indicate that UbcH10 overexpression has a critical role in lung carcinogenesis, and the evaluation of UbcH10 expression levels may be a new tool for the characterisation of NSCLC.
authors.affiliation: Istituto di Endocrinologia ed Oncologia Sperimentale del CNR c/o Dipartimento di Biologia e Patologia Cellulare e Molecolare, Facoltà di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli Federico II, via Pansini 5, Naples, Italy.
authors.forename: Pierlorenzo
authors.initials: P
authors.lastname: Pallante
authors.forename: Umberto
authors.initials: U
authors.lastname: Malapelle
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Berlingieri
authors.forename: Claudio
authors.initials: C
authors.lastname: Bellevicine
authors.forename: Romina
authors.initials: R
authors.lastname: Sepe
authors.forename: Antonella
authors.initials: A
authors.lastname: Federico
authors.forename: Danilo
authors.initials: D
authors.lastname: Rocco
authors.forename: Mario
authors.initials: M
authors.lastname: Galgani
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Chiariotti
authors.forename: Montserrat
authors.initials: M
authors.lastname: Sanchez-Cespedes
authors.forename: Alfredo
authors.initials: A
authors.lastname: Fusco
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Troncone
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D004252:DNA Mutational Analysis; D004843:Epistasis, Genetic / Q000235:genetics / Q000502:physiology; D066246:ErbB Receptors / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016158:Genes, p53 / Q000502:physiology; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009154:Mutation / Q000502:physiology; D046888:Tissue Array Analysis; D044763:Ubiquitin-Conjugating Enzymes / Q000235:genetics* / Q000378:metabolism / Q000502:physiology
chemical_list: D014408:Biomarkers, Tumor; C105156:UBE2C protein, human; D044763:Ubiquitin-Conjugating Enzymes; C512478:EGFR protein, human; D066246:ErbB Receptors