title: Protective effects of Ankaferd blood stopper on aspirin-induced oxidative mucosal damage in a rat model of gastric injury.
abstract: The exposure of gastric mucosa to damaging factors, such as ethanol and some therapeutic drugs, produces pathological changes: inflammatory process, hemorrhagic erosions and even acute ulcers. Ankaferd blood stopper (ABS) comprises a standardized mixture of five different plant extracts. The purpose of our present investigations is to explain the participation of reactive oxygen species in acute gastric mucosal damage by acetylsalicylic acid (ASA) and the effects of new hemostatic agent ABS. Experiments were carried out on 23 male Wistar rats. To assess gastric mucosal damage, biochemical and histopathological data were used. The colorimetric assays were used to determine the malondialdehyde (MDA) and superoxide dismutase (SOD) activity. The level of myeloperoxidase (MPO) activity, the level of nitric oxide (NO) and the proinflammatory cytokine tumor necrosis factor-α (TNF-α) were measured by enzyme-linked immunosorbent assay technique. We demonstrated that the biological effects of ROS were estimated by measuring the tissue and plasma levels of MDA, the products of lipid peroxidation, as well as the activity of SOD and the scavenger of ROS produced by ASA in the experiment group. Moreover, it was found that MPO activity as well as NO and TNF-α levels also demonstrated significant improvement by ABS treatment. The pathogenesis of experimental ASA-induced mucosal damage in rat stomach includes the generation of ROS that seems to play an important role, due to the generation of lipid peroxides, accompanied by the impairment of antioxidative enzyme activity of cells. ABS appeared to attenuate the oxidative and inflammatory changes caused by ASA-induced gastric mucosal damage in rats.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Fatih University, Ankara, Turkey.
authors.forename: Rukiye
authors.initials: R
authors.lastname: Hasgul
authors.affiliation: Department of Biochemistry, Numune Training and Research Hospital, Ankara, Turkey.
authors.forename: Sema
authors.initials: S
authors.lastname: Uysal
authors.affiliation: Department of Pathology, Faculty of Medicine, Fatih University, Ankara, Turkey.
authors.forename: Hacer
authors.initials: H
authors.lastname: Haltas
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Fatih University, Ankara, Turkey.
authors.forename: Sumeyye
authors.initials: S
authors.lastname: Akyol
authors.affiliation: Department of Histology, Faculty of Medicine, Fatih University, Ankara, Turkey.
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Yuksel
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Fatih University, Ankara, Turkey.
authors.forename: Ayse
authors.initials: A
authors.lastname: Gurel
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Fatih University, Ankara, Turkey farmutcu@fatih.edu.tr.
authors.forename: Ferah
authors.initials: F
authors.lastname: Armutcu
mesh_terms: D000818:Animals; D001241:Aspirin / Q000633:toxicity*; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010936:Plant Extracts; D020011:Protective Agents; C539751:ankaferd blood stopper; D001241:Aspirin
keywords: Acetylsalicylic acid; Ankaferd; gastric mucosal damage; inflammation; oxidative stress