title: CACNA1H antibodies associated with headache with neurological deficits and cerebrospinal fluid lymphocytosis (HaNDL).
abstract: BACKGROUND
  Patients with the syndrome of headache with neurological deficits and lymphocytosis (HaNDL) typically present with recurrent and temporary attacks of neurological symptoms and cerebrospinal fluid lymphocytosis.
  
  
  OBJECTIVE
  To identify potential HaNDL-associated antibodies directed against neuronal surface and/or synapse antigens, sera of four HaNDL patients and controls were screened with indirect immunohistochemistry, immunofluorescence, cell-based assay, radioimmunoassay, protein macroarray and enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  Although HaNDL sera did not yield antibodies to any of the well-characterized neuronal surface or synapse antigens, protein macroarray and ELISA studies showed high-titer antibodies to a subunit of the T-type voltage-gated calcium channel (VGCC), CACNA1H, in sera of two HaNDL patients.
  
  
  CONCLUSIONS
  Our results support the notion that ion channel autoimmunity might at least partially contribute to HaNDL pathogenesis and occurrence of neurological symptoms.
authors.affiliation: Department of Neurology, Acibadem University School of Medicine, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Kürtüncü
authors.forename: Dilaver
authors.initials: D
authors.lastname: Kaya
authors.forename: Luigi
authors.initials: L
authors.lastname: Zuliani
authors.forename: Ece
authors.initials: E
authors.lastname: Erdağ
authors.forename: Sema
authors.initials: S
authors.lastname: Içöz
authors.forename: Elif
authors.initials: E
authors.lastname: Uğurel
authors.forename: Filiz
authors.initials: F
authors.lastname: Cavuş
authors.forename: Neşe
authors.initials: N
authors.lastname: Ayşit
authors.forename: Omer
authors.initials: O
authors.lastname: Birişik
authors.forename: Angela
authors.initials: A
authors.lastname: Vincent
authors.forename: Mefkure
authors.initials: M
authors.lastname: Eraksoy
authors.forename: Burçak
authors.initials: B
authors.lastname: Vural
authors.forename: Gülşen
authors.initials: G
authors.lastname: Akman-Demir
authors.forename: Erdem
authors.initials: E
authors.lastname: Tüzün
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D020747:Calcium Channels, T-Type / Q000097:blood / Q000276:immunology*; D002555:Cerebrospinal Fluid / Q000276:immunology*; D005260:Female; D006261:Headache / Q000097:blood / Q000276:immunology*; D006801:Humans; D008218:Lymphocytosis / Q000134:cerebrospinal fluid* / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009422:Nervous System Diseases / Q000097:blood / Q000276:immunology*
chemical_list: D001323:Autoantibodies; C479952:CACNA1H protein, human; D020747:Calcium Channels, T-Type