title: Engineering catalytic properties and thermal stability of plant formate dehydrogenase by single-point mutations.
abstract: The analysis of the 3D model structure of the ternary complex of recombinant formate dehydrogenase from soya Glycine max (EC 1.2.1.2., SoyFDH) with bound NAD+ and an inhibitor azide ion revealed the presence of hydrophobic Phe290 in the coenzyme-binding domain. This residue should shield the enzyme active site from solvent. On the basis of the alignment of plant FDHs sequences, Asp, Asn and Ser were selected as candidates to substitute Phe290. Computer modeling indicated the formation of two (Ser and Asn) or three (Asp) new hydrogen bonds in such mutants. The mutant SoyFDHs were expressed in Escherichia coli, purified and characterized. All amino acid substitutions increased K(м)(HCOO-) from 1.5 to 4.1-5.0 mM, whereas the K(м)(NAD+) values remained almost unchanged in the range from 9.1 to 14.0 μM, which is close to wt-SoyFDH (13.3 μM). The catalytic constants for F290N, F290D and F290S mutants of SoyFDH equaled 2.8, 5.1 and 4.1 s⁻¹, respectively; while that of the wild-type enzyme was 2.9 s⁻¹. The thermal stability of all mutant SoyFDHs was much higher compared with the wild-type enzyme. The differential scanning calorimetry data were in agreement with the results of thermal inactivation kinetics. The mutations F290S, F290N and F290D introduced into SoyFDH increased the T(m) values by 2.9°C, 4.3°C and 7.8°C, respectively. The best mutant F290D exhibited thermal stability similar to that of FDH from the plant Arabidopsis thaliana and exceeded that of the enzymes from the yeast Candida boidinii and the bacterium Moraxella sp. C1.
authors.affiliation: Department of Chemical Enzymology, Chemistry Faculty,  M.V. Lomonosov Moscow State University, 119991  Moscow,  Russia.
authors.forename: Anastasia A
authors.initials: AA
authors.lastname: Alekseeva
authors.forename: Alexey A
authors.initials: AA
authors.lastname: Serenko
authors.forename: Ivan S
authors.initials: IS
authors.lastname: Kargov
authors.forename: Svyatoslav S
authors.initials: SS
authors.lastname: Savin
authors.forename: Sergey Yu
authors.initials: SY
authors.lastname: Kleymenov
authors.forename: Vladimir I
authors.initials: VI
authors.lastname: Tishkov
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D003001:Cloning, Molecular; D004795:Enzyme Stability; D005560:Formate Dehydrogenases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009243:NAD / Q000378:metabolism; D017354:Point Mutation; D011487:Protein Conformation; D016415:Sequence Alignment; D013025:Glycine max / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D013696:Temperature
chemical_list: D009243:NAD; D005560:Formate Dehydrogenases