title: The influence of dietary lipid composition on liver mitochondria from mice following 1 month of calorie restriction.
abstract: To investigate the role mitochondrial membrane lipids play in the actions of CR (calorie restriction), C57BL/6 mice were assigned to four groups (control and three 40% CR groups) and the CR groups were fed diets containing soya bean oil (also in the control diet), fish oil or lard. The fatty acid composition of the major mitochondrial phospholipid classes, proton leak and H(2)O(2) production were measured in liver mitochondria following 1 month of CR. The results indicate that mitochondrial phospholipid fatty acids reflect the PUFA (polyunsaturated fatty acid) profile of the dietary lipid sources. CR significantly decreased the capacity of ROS (reactive oxygen species) production by Complex III but did not markedly alter proton leak and ETC (electron transport chain) enzyme activities. Within the CR regimens, the CR-fish group had decreased ROS production by both Complexes I and III, and increased proton leak when compared with the other CR groups. The CR-lard group showed the lowest proton leak compared with the other CR groups. The ETC enzyme activity measurements in the CR regimens showed that Complex I activity was decreased in both the CR-fish and CR-lard groups. Moreover, the CR-fish group also had lower Complex II activity compared with the other CR groups. These results indicate that dietary lipid composition does influence liver mitochondrial phospholipid composition, ROS production, proton leak and ETC enzyme activities in CR animals.
authors.affiliation: VM Molecular Biosciences, University of California, Davis, CA, USA.
authors.forename: Yana
authors.initials: Y
authors.lastname: Chen
authors.forename: Kevork
authors.initials: K
authors.lastname: Hagopian
authors.forename: Douglas
authors.initials: D
authors.lastname: Bibus
authors.forename: José M
authors.initials: JM
authors.lastname: Villalba
authors.forename: Guillermo
authors.initials: G
authors.lastname: López-Lluch
authors.forename: Plácido
authors.initials: P
authors.lastname: Navas
authors.forename: Kyoungmi
authors.initials: K
authors.lastname: Kim
authors.forename: Roger B
authors.initials: RB
authors.lastname: McDonald
authors.forename: Jon J
authors.initials: JJ
authors.lastname: Ramsey
mesh_terms: D000818:Animals; D001835:Body Weight; D031204:Caloric Restriction; D004032:Diet; D004041:Dietary Fats / Q000494:pharmacology; D004579:Electron Transport; D014450:Electron Transport Complex III / Q000378:metabolism; D004789:Enzyme Activation; D005395:Fish Oils / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000187:drug effects / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism; D009929:Organ Size; D018384:Oxidative Stress; D011522:Protons; D017382:Reactive Oxygen Species / Q000378:metabolism; D013024:Soybean Oil / Q000494:pharmacology; D013997:Time Factors
chemical_list: D004041:Dietary Fats; D005395:Fish Oils; D008563:Membrane Lipids; D024101:Mitochondrial Proteins; D011522:Protons; D017382:Reactive Oxygen Species; D013024:Soybean Oil; D006861:Hydrogen Peroxide; D014450:Electron Transport Complex III; C029310:lard