title: 5-Bromo-2'-deoxyuridine impairs long-term food aversion memory in edible snail.
abstract: We studied the involvement of DNA synthesis into molecular mechanisms of long-term memory. Nucleoside analogue 5-bromo-2'-deoxyuridine (BrdU) is known to incorporate into synthesizing DNA and prevent subsequent DNA replication from this region. To investigate the effect of BrdU administration on long-term memory, terrestrial gastropods edible snails Helix lucorum were trained in the food aversion paradigm. Single-session training (carrot presentation combined with application of 10% quinine solution, three carrot presentations with 10-min intervals) resulted in the formation of long-term memory that persisted for at least 45° days. BrdU administration (250 mg/kg) 30 min before training impaired long-term memory tested 24 h later. Immunohistochemical study revealed BrdU incorporation in the nuclei of identified neurons of defensive behavior.
authors.affiliation: Laboratory of Neurobiology of Memory, PK Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia. o.efimova.1@gmail.com
authors.forename: O I
authors.initials: OI
authors.lastname: Efimova
authors.forename: K V
authors.initials: KV
authors.lastname: Anokhin
mesh_terms: D000818:Animals; D001362:Avoidance Learning / Q000502:physiology*; D001973:Bromodeoxyuridine / Q000378:metabolism / Q000494:pharmacology*; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis* / Q000378:metabolism; D005502:Food; D007150:Immunohistochemistry; D057567:Memory, Long-Term / Q000187:drug effects* / Q000502:physiology; D009474:Neurons / Q000378:metabolism; D012908:Snails; D018709:Statistics, Nonparametric
chemical_list: D004247:DNA; D001973:Bromodeoxyuridine