title: Effect of combined administration of afobazole and 5-HT2b/2c receptor antagonist SB-200646A on neurochemical profile of brain structures in C57Bl/6 and BALB/c mice.
abstract: The effects of combined administration of afobazole and 5-HT(2b/2c)receptor antagonist SB-200646A on the content of monoamines and their metabolites in brain structures of C57Bl/6 and BALB/c mice were studied by the methods of HPLC with electrochemical detection. Combined administration of afobazole and SB-200646A increased the content of epinephrine in the striatum of BALB/c mice (to 230% of the control) and in the hippocampus of both mouse strains. The content of dihydroxyphenylacetic and homovanillic acids and parameters of dopamine metabolism in these structures were reduced. The content of dopamine in the hypothalamus and amygdala was elevated in C57Bl/6, but not in BALB/c mice. These findings attest to the involvement of monoamine systems of the brain in the mechanism of afobazole action and suggest that the enhanced anxiolytic effect of afobazole combination with SB-200646A can be interpreted as a positive modulation of the effect of anxiolytic determined by blockade of 5-HT(2)serotonin receptors.
authors.affiliation: Laboratory of Neurohistology, PK Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: K S
authors.initials: KS
authors.lastname: Rayevsky
authors.forename: V B
authors.initials: VB
authors.lastname: Narkevich
authors.forename: P M
authors.initials: PM
authors.lastname: Klodt
authors.forename: V S
authors.initials: VS
authors.lastname: Kudrin
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology*; D015306:Biogenic Monoamines / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004298:Dopamine / Q000378:metabolism; D004338:Drug Combinations; D006719:Homovanillic Acid / Q000378:metabolism; D007211:Indoles / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009025:Morpholines / Q000494:pharmacology*; D058826:Serotonin 5-HT2 Receptor Agonists / Q000494:pharmacology*; D014508:Urea / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: C410924:2-((2-morpholino)ethylthio)-5-ethoxybenzimidazole; D014151:Anti-Anxiety Agents; D001562:Benzimidazoles; D015306:Biogenic Monoamines; D004338:Drug Combinations; D007211:Indoles; D009025:Morpholines; D058826:Serotonin 5-HT2 Receptor Agonists; D015102:3,4-Dihydroxyphenylacetic Acid; C080864:N-(1-methyl-5-indolyl)-N'-(3-pyridyl)urea; D014508:Urea; D004298:Dopamine; D006719:Homovanillic Acid