title: The ydaO motif is an ATP-sensing riboswitch in Bacillus subtilis.
abstract: We report what is to our knowledge the first natural RNA that regulates gene expression in response to intracellular ATP. Using a biochemical screen, we found that several putative riboswitches bind ATP in vitro. The ydaO motif specifically bound ATP and regulated expression of endogenous and reporter genes in response to ATP concentrations in Bacillus subtilis. This discovery demonstrates a role for RNAs in regulating gene expression in response to energy balance in bacteria.
authors.affiliation: Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California, USA.
authors.forename: Peter Y
authors.initials: PY
authors.lastname: Watson
authors.forename: Martha J
authors.initials: MJ
authors.lastname: Fedor
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D020816:Amino Acid Motifs / Q000235:genetics*; D001412:Bacillus subtilis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003956:Dialysis; D004734:Energy Metabolism / Q000235:genetics / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics / Q000502:physiology / Q000528:radiation effects; D006706:Homeostasis; D009154:Mutation / Q000502:physiology; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D058928:Riboswitch / Q000235:genetics* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D012329:RNA, Bacterial; D058928:Riboswitch; D000255:Adenosine Triphosphate