title: The use of type 1 collagen scaffold containing stromal cell-derived factor-1 to create a matrix environment conducive to partial-thickness cartilage defects repair.
abstract: Despite the presence of cartilage-derived mesenchymal stem cells (C-MSCs) and synovial membrane-derived mesenchymal stem cells (SM-MSCs) populations, partial-thickness cartilage defects, in contrast to the full-thickness defects, are devoid of spontaneous repair capacity. This study aims to create an in situ matrix environment conducive to C-MSCs and SM-MSCs to promote cartilage self-repair. Spontaneous repair with MSCs migration into the defect area was observed in full-thickness defects, but not in partial-thickness defects in rabbit model. Ex vivo and in vitro studies showed that subchondral bone or type 1 collagen (col1) scaffold was more permissive for MSCs adhesion than cartilage or type 2 collagen (col2) scaffold and induced robust stromal cell-derived factors-1 (SDF-1) dependent migration of MSCs. Furthermore, creating a matrix environment with col1 scaffold containing SDF-1 enhanced in situ self-repair of partial-thickness defects in rabbit 6 weeks post-injury. Hence, the inferior self-repair capacity in partial-thickness defects is partially owing to the non-permissive matrix environment. Creating an in situ matrix environment conducive to C-MSCs and SM-MSCs migration and adhesion with col1 scaffold containing SDF-1 can be exploited to improve self-repair capacity of cartilage.
authors.affiliation: Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, #866 Yu Hang Tang Road, Hangzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Jialin
authors.initials: J
authors.lastname: Chen
authors.forename: Jiadong
authors.initials: J
authors.lastname: Tao
authors.forename: Yangzi
authors.initials: Y
authors.lastname: Jiang
authors.forename: Changchang
authors.initials: C
authors.lastname: Hu
authors.forename: Lu
authors.initials: L
authors.lastname: Huang
authors.forename: Junfeng
authors.initials: J
authors.lastname: Ji
authors.forename: Hong Wei
authors.initials: HW
authors.lastname: Ouyang
mesh_terms: D000368:Aged; D000818:Animals; D002356:Cartilage / Q000166:cytology* / Q000293:injuries* / Q000648:ultrastructure; D002448:Cell Adhesion; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000008:administration & dosage* / Q000494:pharmacology; D024042:Collagen Type I / Q000737:chemistry*; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D008875:Middle Aged; D011817:Rabbits; D013552:Swine; D013583:Synovial Membrane / Q000166:cytology*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing
chemical_list: D054377:Chemokine CXCL12; D024042:Collagen Type I