title: [Sporothrix globosa isolation related to a case of lymphocutaneous sporotrichosis].
abstract: BACKGROUND
  Sporothrix schenckii complex comprises a group of environmental dimorphic fungi that cause sporotrichosis. In Chile, isolated cases have been reported in humans, though no environmental isolates have been described.
  
  
  OBJECTIVE
  To achieve isolation of Sporothrix complex from the soil where a 75 year old patient with lymphocutaneous sporotrichosis performs horticulture work.
  
  
  METHODS
  In March and July 2011 soil and plant debris from five sectors where the patient does his work in horticulture was extracted. The soil samples were diluted and inoculated in Sabouraud agar with cycloheximide and chloramphenicol at 26 °C. The plant debris was directly inoculated in the same medium. Colonies suggestive of Sporothrix complex were reseeded in PDA agar at 26 ° C and identified as recommended by Marimon et al.
  
  
  RESULTS
  Of the 10 plates from the first sampling, one colony was identified as Sporothrix globosa. In the second sampling, Sporothrix globosa grew in two plates seeded with soil, with a total of 6 colonies. There was no growth of Sporothrix complex in plant debris. The isolate from the patient was also identified as Sporothrix globosa.
  
  
  CONCLUSIONS
  For the first time in Chile a species of Sporothrix complex was isolated from the environment. Sporothrix globosa was the species identified both in the ground and from the patient with sporotrichosis.
authors.affiliation: Laboratorio de Micología, Universidad de Valparaíso, Chile. rcruzchoappa@gmail.com
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Cruz
authors.forename: Peggy
authors.initials: P
authors.lastname: Vieille
authors.forename: David
authors.initials: D
authors.lastname: Oschilewski
mesh_terms: D000368:Aged; D000935:Antifungal Agents / Q000627:therapeutic use; D001132:Arm; D002677:Chile; D015169:Colony Count, Microbial; D005260:Female; D006225:Hand; D006801:Humans; D017964:Itraconazole / Q000627:therapeutic use; D012988:Soil Microbiology; D013175:Sporothrix / Q000145:classification / Q000302:isolation & purification*; D013174:Sporotrichosis / Q000175:diagnosis / Q000382:microbiology*; D016896:Treatment Outcome
chemical_list: D000935:Antifungal Agents; D017964:Itraconazole