title: Calibration of glucose oxidase-based test strips for capillary blood measurement with oxygen saturated venous blood samples.
abstract: BACKGROUND
  Glucose oxidase biosensors are used in self-monitoring blood glucose concentrations. The capillary blood glucose quantitation requires a calibration curve. Due to the limitation in obtaining calibration curve from capillary blood, an alternate approach by using venous blood for neonatal measurement was investigated.
  
  
  METHODS
  A signal correlation between oxygen saturated venous blood and capillary blood was derived. The hematocrit effect was studied for different glucose concentrations. The calibrated glucose strips were validated by neonatal intensive care unit (NICU) samples.
  
  
  RESULTS
  A simple equation, finger blood signal=1.39∗(oxygen saturated venous blood signal)-31.2 was derived. The rate of change in glucose concentration due to hematocrit effect was low in lower glucose concentration samples. The BeneCheck Glucose Strips were compared with Beckman Coulter analyzer by using 52 NICU samples. More than 95% of test results were within the variation of ±10 mg/dl of bias and ±15% of bias% when glucose concentration is <75 mg/dl and ≥75 mg/dl respectively.
  
  
  CONCLUSIONS
  The BeneCheck Glucose Strips can be accurately calibrated with venous blood. The hematocrit effect can also be predicted. Based on this study, BeneCheck Blood Glucose Monitoring System can be suitable for neonatal glucose measurement.
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, No. 1 Sec. 4, Roosevelt Road, Taipei, 10617, Taiwan.
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Cheng
authors.forename: Chii-Wann
authors.initials: CW
authors.lastname: Lin
authors.forename: Tai-Guang
authors.initials: TG
authors.lastname: Wu
authors.forename: Chein-Shyong
authors.initials: CS
authors.lastname: Su
authors.forename: Ming-Song
authors.initials: MS
authors.lastname: Hsieh
mesh_terms: D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D002138:Calibration; D002196:Capillaries / Q000737:chemistry*; D005385:Fingers / Q000098:blood supply; D005949:Glucose Oxidase / Q000737:chemistry*; D006400:Hematocrit; D006801:Humans; D007231:Infant, Newborn; D010100:Oxygen / Q000737:chemistry; D018962:Phlebotomy; D019095:Point-of-Care Systems; D011934:Reagent Strips / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014699:Venules / Q000737:chemistry*
chemical_list: D001786:Blood Glucose; D011934:Reagent Strips; D005949:Glucose Oxidase; D010100:Oxygen