title: Microfluidic artificial "vessels" for dynamic mechanical stimulation of mesenchymal stem cells.
abstract: Cells in the cardiovascular system are constantly exposed to complex mechanical stimulation due to the pulsatile nature of blood flow and the haemodynamic forces that are key to the regulation of vascular development, remodeling and pathophysiology. Mechanical stretch can also modulate the differentiation of stem cells toward vascular cell lineages (i.e., vascular smooth muscle cells), and represent a critical factor in vascular tissue engineering. Here we report on the development of a microchip platform that can emulate several key aspects of the vascular mechanical environment, such as cyclic stimulation and circumferential strain. This chip consists of an array of microfluidic channels with widths ranging from 20 to 500 micrometers. These channels are covered by suspended deformable membranes, on which cells are cultured and stimulated by cyclic circumferential strain of up to 20% via hydrodynamic actuation of the fluid in the microfluidic channels, thereby mimicking the biomechanical conditions of small blood vessels. We show that human mesenchymal stem cells (MSCs) can be cultured and continuously stimulated by cyclic stretch over a period of 7 days with no evidence of device fatigue or performance degradation. We observed localization and alignment of MSCs when mechanical stretch is larger than 10%, indicating the importance of mechanical stimulation in modulating cellular behavior. We further demonstrated simultaneous detection of proteins in multiple signaling pathways, including SMAD1/SMAD2 and canonical Wnt/β-catenin. This microchip represents a generic and versatile platform for high-throughput and rapid screening of cellular responses, including signal transduction cascades, in response to mechanical cues. The system emulates the physiological conditions of blood vessels and other tissues that are subject to cyclic strain, and may have a wide range of applications in the fields of stem cell mechanobiology, vascular tissue engineering, and other areas of regenerative medicine.
authors.affiliation: Department of Anesthesiology, Yale School of Medicine, New Haven, CT 06519, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.forename: Laura E
authors.initials: LE
authors.lastname: Niklason
mesh_terms: D001696:Biomechanical Phenomena; D001808:Blood Vessels / Q000166:cytology / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D004867:Equipment Design; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D046210:Microfluidic Analytical Techniques; D015398:Signal Transduction; D051785:Smad Proteins / Q000502:physiology; D013314:Stress, Mechanical; D049490:Systems Biology; D023822:Tissue Engineering; D016212:Transforming Growth Factor beta / Q000502:physiology; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000502:physiology
chemical_list: C495270:CTNNB1 protein, human; D051785:Smad Proteins; D016212:Transforming Growth Factor beta; D051176:beta Catenin