title: Acute effects of resistance exercise and intermittent intense aerobic exercise on blood cell count and oxidative stress in trained middle-aged women.
abstract: The aim of this study was to compare the effect of an intermittent intense aerobic exercise session and a resistance exercise session on blood cell counts and oxidative stress parameters in middle-aged women. Thirty-four women were selected and divided into three groups: RE group (performing 60 min of resistance exercises, N = 12), spinning group (performing 60 min of spinning, N = 12), and control group (not exercising regularly, N = 10). In both exercise groups, lymphocytes and monocytes decreased after 1-h recuperation (post-exercise) compared to immediately after exercise (P < 0.05). Immediately after exercise, in both exercised groups, a significant increase in TBARS (from 16.5 ± 2 to 25 ± 2 for the spinning group and from 18.6 ± 1 to 28.2 ± 3 nmol MDA/mL serum for the RE group) and protein carbonyl (from 1.0 ± 0.3 to 1.6 ± 0.2 for the spinning group and from 0.9 ± 0.2 to 1.5 ± 0.2 nmol/mg protein for the RE group) was observed (P < 0.05). A decrease in antioxidant activities (non-protein sulfhydryl, superoxide dismutase, catalase) was also demonstrated with a negative correlation between damage markers and antioxidant body defenses (P < 0.05). These results indicate that an acute bout of intermittent or anaerobic exercise induces immune suppression and increases the production of reactive oxygen species, causing oxidative stress in middle-aged and trained women. Furthermore, we demonstrated that trained women show improved antioxidant capacity and lower oxidative damage than sedentary ones, demonstrating the benefits of chronic regular physical activity.
authors.affiliation: Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors.forename: A M
authors.initials: AM
authors.lastname: Cardoso
authors.forename: M D
authors.initials: MD
authors.lastname: Bagatini
authors.forename: M A
authors.initials: MA
authors.lastname: Roth
authors.forename: C C
authors.initials: CC
authors.lastname: Martins
authors.forename: J F P
authors.initials: JF
authors.lastname: Rezer
authors.forename: F F
authors.initials: FF
authors.lastname: Mello
authors.forename: L F D
authors.initials: LF
authors.lastname: Lopes
authors.forename: V M
authors.initials: VM
authors.lastname: Morsch
authors.forename: M R C
authors.initials: MR
authors.lastname: Schetinger
mesh_terms: D015415:Biomarkers / Q000097:blood; D001772:Blood Cell Count; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood; D005080:Exercise Test; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000097:blood*; D055070:Resistance Training; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D015415:Biomarkers; D017382:Reactive Oxygen Species; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase