title: Altered polarization, morphology, and impaired innate immunity germane to resident peritoneal macrophages in mice with long-term type 2 diabetes.
abstract: Type 2 diabetes (T2D) is associated with perturbed innate immunity. Macrophages, bridging innate immunity and metabolic disturbances, play important roles in controlling immune homeostasis. However, the effect of long-term diabetic milieu (DM) on the functions and phenotypes of macrophages is still not clear. In this study, we used resident peritoneal macrophages (RPMs) from 5-month-old db/db mice to investigate the changes of macrophages. It was found that RPMs in db/db mice significantly reduced phagocytosis and adhesion capacity. After standardization with body weight, the number of F4/80(+) RPMs markedly reduced in db/db mice, and, furthermore, the macrophages skewed to M2-polarizated macrophages. The results of morphology found that the RPMs shape of db/db mice was nearly round, but the RPMs shape of control mice was spindle-shaped and irregular. In this study, we found the cell numbers, morphology, and innate immunity functions of RPMs in 5-month-old type 2 diabetic mice (db/db mice) obtained by abdominal cavity lavage were significantly altered. Importantly, we also found the remarkably increased M2-RPMs in diabetic mice for the first time.
authors.affiliation: Department of Endocrinology, The Shanghai Third People's Hospital, Shanghai Jiao Tong University School of Medicine, 280 Mohe Road, Shanghai 201900, China.
authors.forename: Hui-Fang
authors.initials: HF
authors.lastname: Liu
authors.forename: Hui-Jie
authors.initials: HJ
authors.lastname: Zhang
authors.forename: Qi-Xian
authors.initials: QX
authors.lastname: Hu
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Liu
authors.forename: Zhi-Quan
authors.initials: ZQ
authors.lastname: Wang
authors.forename: Jia-Yan
authors.initials: JY
authors.lastname: Fan
authors.forename: Ming
authors.initials: M
authors.lastname: Zhan
authors.forename: Feng-Ling
authors.initials: FL
authors.lastname: Chen
mesh_terms: D000818:Animals; D016764:Cell Polarity; D048429:Cell Size; D002478:Cells, Cultured; D003924:Diabetes Mellitus, Type 2 / Q000276:immunology*; D007113:Immunity, Innate / Q000276:immunology*; D008264:Macrophages / Q000276:immunology* / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010537:Peritoneum / Q000276:immunology* / Q000473:pathology*