title: Healing and expression of growth factors (TGF-β and PDGF) in canine radial ostectomy gap containing platelet-rich plasma.
abstract: OBJECTIVE
  To evaluate bone healing in an experimental radial ostectomy in dogs treated with autologous platelet-rich plasma (PRP), through histological, densitometric, radiographic studies, as well as expression of growth factors in the ostectomy gap.
  
  
  METHODS
  Twenty-one dogs were randomly divided into either a control or a PRP group. All underwent unilateral ostectomy of the radius to generate a gap of 2.0 mm, that was stabilized with external skeletal fixation. The ostectomy gap was either filled with PRP or left empty as a control. The radiographic and densitometric studies were performed after surgery, then at intervals until 60 days during the post-surgery period. Histological and immunohistochemical evaluations were performed at seven or 60 days post-surgery. Analyses were performed using a statistical analysis system, and the level of significance was set at p <0.05.
  
  
  RESULTS
  The median radiographic healing score in the PRP group increased significantly between day 0 and day 60. Furthermore, at 60 days, the median healing score and the proportion of healed ostectomies in the control group (score 1; 1/6 healed) and the PRP group (score 5; 4/5 healed) were significantly different. There were differences between groups in radiographic and densitometric values at days 45 and 60. The histological evaluation showed advanced bone healing at 60 days in the PRP group and signs of delayed union in the control group.
  
  
  CONCLUSIONS
  Platelet-rich plasma can be used as an adjuvant therapy because it may promote better bone healing of a radial ostectomy treated with external skeletal fixation in dogs.
authors.affiliation: Veterinary Medicine Faculty of Araçatuba, Department of Clinic, Surgery and Animal Reproduction, Araçatuba, SP, Brazil.
authors.forename: T F B
authors.initials: TF
authors.lastname: Souza
authors.forename: A L
authors.initials: AL
authors.lastname: Andrade
authors.forename: G T N M
authors.initials: GT
authors.lastname: Ferreira
authors.forename: G T N M
authors.initials: GT
authors.lastname: Ferrreira
authors.forename: S S
authors.initials: SS
authors.lastname: Sakamoto
authors.forename: V B
authors.initials: VB
authors.lastname: Albuquerque
authors.forename: S R M
authors.initials: SR
authors.lastname: Bonfim
authors.forename: M C R
authors.initials: MC
authors.lastname: Luvizotto
authors.forename: M J Q
authors.initials: MJ
authors.lastname: Louzada
mesh_terms: D020341:Absorbable Implants / Q000662:veterinary; D000818:Animals; D001696:Biomechanical Phenomena; D001842:Bone and Bones / Q000502:physiology*; D004285:Dogs; D005260:Female; D005552:Forelimb; D017102:Fracture Healing / Q000502:physiology*; D020007:Implants, Experimental / Q000662:veterinary*; D008297:Male; D010982:Platelet-Derived Growth Factor / Q000235:genetics / Q000378:metabolism*; D053657:Platelet-Rich Plasma; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism*; D053781:Transforming Growth Factor beta2 / Q000235:genetics / Q000378:metabolism*
chemical_list: D010982:Platelet-Derived Growth Factor; D053773:Transforming Growth Factor beta1; D053781:Transforming Growth Factor beta2