title: Effects of Er'zhi Tiangui Granule () on sequential expressions of integrin β 3 and its ligand osteopontin in mouse endometrium during controlled ovarian hyperstimulation.
abstract: OBJECTIVE
  To investigate the effects of Er'zhi Tiangui Granule (, ETG) on sequential expressions of integrinβ3 and its ligand osteopontin in the mouse endometrium during controlled ovarian hyperstimulation (COH) and implantation period.
  
  
  METHODS
  Seventy-five Mature female Kunming mice were randomly divided into 3 groups, a normal control group, a model group, and a treatment group administrated with ETG for 10 days, 25 in each group. After mated with male mice, every 5 mice were sacrified in each group at the 0, 2nd, 4th, 6th, and 8th days to take their endometrium. In-situ hybridization was used to detect the expressions of integrinβ3 and osteopontin in the endometrium.
  
  
  RESULTS
  mRNA expressions of integrinβ3 and osteopontin in the endometrium during implantation period showed similar time sequence rules in the treatment group to those in the normal control group; the peak values of them were a little lower in the treatment group than the normal control without significant differences. In the model group, integrinβ3 mRNA expression was higher at the 2nd day, obviously lower at the 4th and 6th days, and insignificantly lower at the 8th day; and osteopontin expression was remarkably lower at the 4th, 6th, and 8th days, compared with the normal control and the treatment groups (P<0.05, P<0.01).
  
  
  CONCLUSIONS
  COH might influence the sequential expressions of integrinβ3 and its ligand osteopontin, bring forward the integrinβ3 expression peak, impact on the cooperation of integrinβ3 and osteopontin, so as to damage the endometrial receptivity. ETG could regulate the sequential expressions of integrinβ3 and its ligand osteopontin to improve the mouse endometrial receptivity during COH.
authors.affiliation: Reproductive Center, the Affiliated Hospital of Shandong, University of Traditional Chinese Medicine, Jinan, China.
authors.forename: Zhen-Gao
authors.initials: ZG
authors.lastname: Sun
authors.forename: Fang
authors.initials: F
authors.lastname: Lian
authors.forename: Qing
authors.initials: Q
authors.lastname: Jia
authors.forename: Jin-Long
authors.initials: JL
authors.lastname: Sun
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Guo
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Zhang
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Wang
mesh_terms: D000818:Animals; D004304:Dosage Forms; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D004717:Endometrium / Q000187:drug effects* / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D039661:Integrin beta3 / Q000235:genetics* / Q000378:metabolism; D008024:Ligands; D008297:Male; D051379:Mice; D053495:Osteopontin / Q000235:genetics* / Q000378:metabolism; D010062:Ovulation Induction / Q000662:veterinary
chemical_list: D004304:Dosage Forms; D004365:Drugs, Chinese Herbal; D039661:Integrin beta3; D008024:Ligands; C506896:Spp1 protein, mouse; C579122:er'zhi tiangui; D053495:Osteopontin