title: Human serum albumin stabilized gold nanoclusters as selective luminescent probes for Staphylococcus aureus and methicillin-resistant Staphylococcus aureus.
abstract: In this work, human serum albumin (HSA) stabilized gold nanoclusters (HSA-AuNCs) with reddish photoluminescence were used as sensing probes for pathogenic bacteria including Enterobacter cloacae, Escherichia coli J96, Pseudomonas aeruginosa, pandrug-resistant Acinetobacter baumannii (PDRAB), Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), Streptococcus pyogenes, and vancomycin-resistant Enterococcus faecalis (VRE). We discovered that HSA-AuNCs have unique affinity with S. aureus and MRSA. In addition to demonstrating the selective sensing ability of HSA-AuNCs toward S. aureus and MRSA, the binding peptide motifs identified from HSA-AuNCs were characterized by mass spectrometry. The identified binding peptides were further used as the reducing and stabilizing agents for generation of peptide-bound AuNCs (Pep-AuNCs). The generated Pep-AuNCs were demonstrated to have the binding affinities with S. aureus and MRSA.
authors.forename: Po-Han
authors.initials: PH
authors.lastname: Chan
authors.forename: Yu-Chie
authors.initials: YC
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D006046:Gold / Q000737:chemistry*; D006801:Humans; D049408:Luminescent Agents / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification* / Q000378:metabolism; D015335:Molecular Probes / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D055550:Protein Stability; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism; D013211:Staphylococcus aureus / Q000302:isolation & purification* / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
chemical_list: D049408:Luminescent Agents; D015335:Molecular Probes; D012709:Serum Albumin; D006046:Gold; D014357:Trypsin