title: Constrained and UV-activatable cell-penetrating peptides for intracellular delivery of liposomes.
abstract: Herein we report on the development of a novel method of constraining a cell-penetrating peptide, which can be used to trigger transport of liposomes into cells upon in this case radiation with UV-light. A cell-penetrating peptide, which was modified on both termini with an alkyl chain, was anchored to the liposomal surface in a constrained and deactivated form. Since one of the two alkyl chains was connected to the peptide via a UV-cleavable linker, disconnection of this alkyl chain upon irradiation led to the exposure of the cell-penetrating peptide, and mediated the transport of the entire liposome particle into cells.
authors.affiliation: Radboud University Nijmegen, Institute for Molecules and Materials, Department of Organic Chemistry, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.
authors.forename: Morten B
authors.initials: MB
authors.lastname: Hansen
authors.forename: Ethlinn
authors.initials: E
authors.lastname: van Gaal
authors.forename: Inge
authors.initials: I
authors.lastname: Minten
authors.forename: Gert
authors.initials: G
authors.lastname: Storm
authors.forename: Jan C M
authors.initials: JC
authors.lastname: van Hest
authors.forename: Dennis W P M
authors.initials: DW
authors.lastname: Löwik
mesh_terms: D002448:Cell Adhesion; D018929:Cell Culture Techniques; D057846:Cell-Penetrating Peptides / Q000737:chemistry* / Q000493:pharmacokinetics / Q000528:radiation effects; D004337:Drug Carriers / Q000737:chemistry* / Q000493:pharmacokinetics / Q000528:radiation effects; D004705:Endocytosis; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes; D018613:Microscopy, Confocal; D015394:Molecular Structure; D012542:Scattering, Radiation; D013499:Surface Properties; D014466:Ultraviolet Rays; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000528:radiation effects
chemical_list: D057846:Cell-Penetrating Peptides; D004337:Drug Carriers; D008081:Liposomes; D054322:tat Gene Products, Human Immunodeficiency Virus