title: Structure and immunology of a heteroglycan from somatic hybrid mushroom of Pleurotus florida and Calocybe indica var. APK2.
abstract: A water soluble polysaccharide was isolated from the aqueous extract of the fruit bodies of somatic hybrid mushroom PCH3FB, obtained through protoplast fusion between the strains Pleurotus florida and Calocybe indica var. APK2. On the basis of sugar analysis, methylation analysis, periodate oxidation, and NMR studies ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC and HMBC), the structure of the repeating unit of the polysaccharide was established as: [formula, see text] This molecule showed macrophage, splenocyte and thymocyte activations.
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Biswajit
authors.initials: B
authors.lastname: Dey
authors.forename: Sanjoy K
authors.initials: SK
authors.lastname: Bhunia
authors.forename: Kankan K
authors.initials: KK
authors.lastname: Maity
authors.forename: Sukesh
authors.initials: S
authors.lastname: Patra
authors.forename: Soumitra
authors.initials: S
authors.lastname: Mandal
authors.forename: Birendra
authors.initials: B
authors.lastname: Behera
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Maiti
authors.forename: Samir R
authors.initials: SR
authors.lastname: Sikdar
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002478:Cells, Cultured; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000166:cytology / Q000276:immunology*; D051379:Mice; D020076:Pleurotus / Q000737:chemistry*; D060168:Thymocytes / Q000166:cytology / Q000276:immunology*
chemical_list: D062610:Fungal Polysaccharides