title: The structural dynamics of the flavivirus fusion peptide-membrane interaction.
abstract: Membrane fusion is a crucial step in flavivirus infections and a potential target for antiviral strategies. Lipids and proteins play cooperative roles in the fusion process, which is triggered by the acidic pH inside the endosome. This acidic environment induces many changes in glycoprotein conformation and allows the action of a highly conserved hydrophobic sequence, the fusion peptide (FP). Despite the large volume of information available on the virus-triggered fusion process, little is known regarding the mechanisms behind flavivirus-cell membrane fusion. Here, we evaluated the contribution of a natural single amino acid difference on two flavivirus FPs, FLA(G) ((98)DRGWGNGCGLFGK(110)) and FLA(H) ((98)DRGWGNHCGLFGK(110)), and investigated the role of the charge of the target membrane on the fusion process. We used an in silico approach to simulate the interaction of the FPs with a lipid bilayer in a complementary way and used spectroscopic approaches to collect conformation information. We found that both peptides interact with neutral and anionic micelles, and molecular dynamics (MD) simulations showed the interaction of the FPs with the lipid bilayer. The participation of the indole ring of Trp appeared to be important for the anchoring of both peptides in the membrane model, as indicated by MD simulations and spectroscopic analyses. Mild differences between FLA(G) and FLA(H) were observed according to the pH and the charge of the target membrane model. The MD simulations of the membrane showed that both peptides adopted a bend structure, and an interaction between the aromatic residues was strongly suggested, which was also observed by circular dichroism in the presence of micelles. As the FPs of viral fusion proteins play a key role in the mechanism of viral fusion, understanding the interactions between peptides and membranes is crucial for medical science and biology and may contribute to the design of new antiviral drugs.
authors.affiliation: Programa de Biologia Estrutural, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Ygara S
authors.initials: YS
authors.lastname: Mendes
authors.forename: Nathalia S
authors.initials: NS
authors.lastname: Alves
authors.forename: Theo L F
authors.initials: TL
authors.lastname: Souza
authors.forename: Ivanildo P
authors.initials: IP
authors.lastname: Sousa
authors.forename: M Lucia
authors.initials: ML
authors.lastname: Bianconi
authors.forename: Rafael C
authors.initials: RC
authors.lastname: Bernardi
authors.forename: Pedro G
authors.initials: PG
authors.lastname: Pascutti
authors.forename: Jerson L
authors.initials: JL
authors.lastname: Silva
authors.forename: Andre M O
authors.initials: AM
authors.lastname: Gomes
authors.forename: Andréa C
authors.initials: AC
authors.lastname: Oliveira
mesh_terms: D000595:Amino Acid Sequence; D002942:Circular Dichroism; D005416:Flavivirus / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008051:Lipid Bilayers / Q000737:chemistry*; D008561:Membrane Fusion; D008823:Micelles; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis*; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D055672:Static Electricity; D014364:Tryptophan / Q000737:chemistry*; D014760:Viral Fusion Proteins / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D008823:Micelles; D010455:Peptides; D014760:Viral Fusion Proteins; D014364:Tryptophan