title: Gold nanoparticles downregulate interleukin-1β-induced pro-inflammatory responses.
abstract: Interleukin 1 beta (IL-1β)-dependent inflammatory disorders, such as rheumatoid arthritis and psoriasis, pose a serious medical burden worldwide, where patients face a lifetime of illness and treatment. Organogold compounds have been used since the 1930s to treat rheumatic and other IL-1β-dependent diseases and, though their mechanisms of action are still unclear, there is evidence that gold interferes with the transmission of inflammatory signalling. Here we show for the first time that citrate-stabilized gold nanoparticles, in a size dependent manner, specifically downregulate cellular responses induced by IL-1β both in vitro and in vivo. Our results indicate that the anti-inflammatory activity of gold nanoparticles is associated with an extracellular interaction with IL-1β, thus opening potentially novel options for further therapeutic applications.
authors.affiliation: Medway School of Pharmacy, University of Kent, Chatham Maritime, Kent, UK. V.Sumbayev@kent.ac.uk
authors.forename: Vadim V
authors.initials: VV
authors.lastname: Sumbayev
authors.forename: Inna M
authors.initials: IM
authors.lastname: Yasinska
authors.forename: Cesar Pascual
authors.initials: CP
authors.lastname: Garcia
authors.forename: Douglas
authors.initials: D
authors.lastname: Gilliland
authors.forename: Gurprit S
authors.initials: GS
authors.lastname: Lall
authors.forename: Bernhard F
authors.initials: BF
authors.lastname: Gibbs
authors.forename: David R
authors.initials: DR
authors.lastname: Bonsall
authors.forename: Luca
authors.initials: L
authors.lastname: Varani
authors.forename: François
authors.initials: F
authors.lastname: Rossi
authors.forename: Luigi
authors.initials: L
authors.lastname: Calzolai
mesh_terms: D000818:Animals; D015153:Blotting, Western; D020170:Caspase 1 / Q000378:metabolism; D002460:Cell Line; D004789:Enzyme Activation / Q000187:drug effects; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology*; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D006046:Gold; D019869:Phosphatidylinositol 3-Kinases; D020170:Caspase 1