title: Identification of the major proteins of the virions of bacteriophage ZF40 Pectobacterium carotovorum.
abstract: The vast variety of bacteriophages and the uniqueness of their individual representatives dictate to perform the detailed study of the actual phage-cell interactions, the virion morphogenesis and morphopoiesis in particular. An analysis of the complete genome sequence of the temperate phage ZF40 Pectobacterium carotovorum has shown that it is a representative of a unique group of phages of the Myoviridae family [Comeau A. M, Tremblay D., Moineau S., Rattei T., Kushkina A. I, Tovkach F I., H.M. Krisch, H.W. Ackermann Phage Morphology Recapitulates Phylogeny: The Comparative Genomics of a New Group of Myoviruses // PLoS ONE.--July 2012. - 7. - N 7. - e40102]. Characteristic features of these viruses are a small length of the tail compared with the diameter of the capsid and a complicated pattern of the tail sheath, leading to its criss-cross striation. In the presented article the major proteins were identified by means of the SDS-PAGE method: the head proteins (mp2: 33.9 kDa), the sheath (mp1: 39.2 kDa) and the tail tube ones (mp3: 19.9 kDa). It was proved that the mp2 molecular weight is the same with the gp46, the putative major capsid protein derived from the results of the genome sequencing. Therefore, it is still not determined whether the gp46 (mp2) of the virulent mutant 421 of the phage ZF40 is exposed to post-translational modification in the course of the phage particle maturation during its development in the cells of the strain M2-4/50RI P. carotovorum. To study the morphogenetic development pathways it was proposed to use the phage variants that form an excess of individual components of the virion: capsids, procapsids and separate tails propagated on different hosts.
authors.affiliation: Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Acad. Zabolotny St., Kyiv, MSP D03680, Ukraine.
authors.forename: N A
authors.initials: NA
authors.lastname: Korol
authors.forename: F I
authors.initials: FI
authors.lastname: Tovkach
mesh_terms: D001435:Bacteriophages / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D002213:Capsid; D036022:Capsid Proteins / Q000737:chemistry* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D008970:Molecular Weight; D017900:Myoviridae / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D016973:Pectobacterium carotovorum / Q000821:virology; D017376:Viral Tail Proteins / Q000737:chemistry* / Q000302:isolation & purification; D014771:Virion / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure
chemical_list: D036022:Capsid Proteins; D017376:Viral Tail Proteins