title: Antioxidant and lipid lowering activities of Indian black tea.
abstract: Indian black tea; CTC leaf and dust, produced by Tata Tea Limited, Kolkata, (India) was studiedin vitro as potential scavenger of oxygen free radicals. Super oxide anions were generated in a system containing xanthine-xanthine oxidase (enzymic system) and by NADH- phenozine methosulphate (non enzymic system). Anions were assayed in terms of uric acid formation and reduction of nitroblue tetrazolium salt, which were shown to be suppressed by tea extracts. Extracts from both leaf and dust also inhibted the formation of hydroxyl radicalsin vitro in the enzymic system comprising hypoxanthine-Cu(+2)-sodium ascorbate and xanthine oxidase and in non enzymic system of deoxyribose-Cu(+2)-sodium ascorbate and H(2)O(2) as well as the Cu(+2) induced lipid peroxidation in human low density lipoprotein. Feeding with black tea in normal rats for sixty days increased their antioxidant activity and their liver microsomes were shown to be protected against peroxidation of lipids as stimulated by metal ions with enzymic or non enzymic reactants. Furthermore feeding with tea extracts in normal as well as triton WR-1339 induced hyperlipidemic rats caused decrease in their plasma levels of total cholesterol, phospholipids and triglycerides. The antioxidant and lipid lowering activities of both extracts from CTC leaf and dust tea was comparable and may be due to the presence of natural products like catechin and others.
authors.affiliation: Division of Biochemistry, CDRI, 226001 Lucknow.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Chander
authors.forename: A K
authors.initials: AK
authors.lastname: Khanna
authors.forename: Kanwal
authors.initials: K
authors.lastname: Raj
authors.forename: A K
authors.initials: AK
authors.lastname: Rastogi
keywords: Antioxidant activity; Free radicals; Indian Black Tea; Low density liopoprotein oxidation; Triton models