title: Thermodynamics and molecular dynamics simulations of calcium binding to the regulatory site of human cardiac troponin C: evidence for communication with the structural calcium binding sites.
abstract: Human cardiac troponin C (HcTnC), a member of the EF hand family of proteins, is a calcium sensor responsible for initiating contraction of the myocardium. Ca(2+) binding to the regulatory domain induces a slight change in HcTnC conformation which modifies subsequent interactions in the troponin-tropomyosin-actin complex. Herein, we report a calorimetric study of Ca(2+) binding to HcTnC. Isotherms obtained at 25 °C (10 mM 2-morpholinoethanesulfonic acid, 50 mM KCl, pH 7.0) provided thermodynamic parameters for Ca(2+) binding to both the high-affinity and the low-affinity domain of HcTnC. Ca(2+) binding to the N-domain was shown to be endothermic in 2-morpholinoethanesulfonic acid buffer and allowed us to extract the thermodynamics of Ca(2+) binding to the regulatory domain. This pattern stems from changes that occur at the Ca(2+) site rather than structural changes of the protein. Molecular dynamics simulations performed on apo and calcium-bound HcTnC(1-89) support this claim. The values of the Gibbs free energy for Ca(2+) binding to the N-domain in the full-length protein and to the isolated domain (HcTnC(1-89)) are similar; however, differences in the entropic and enthalpic contributions to the free energy provide supporting evidence for the cooperativity of the C-domain and the N-domain. Thermograms obtained at two additional temperatures (10 and 37 °C) revealed interesting trends in the enthalpies and entropies of binding for both thermodynamic events. This allowed the determination of the change in heat capacity (∆C(p)) from a plot of ∆H verses temperature and may provide evidence for positive cooperativity of Ca(2+) binding to the C-domain.
authors.affiliation: Department of Chemistry, East Carolina University, 300 Science and Technology, Greenville, NC 27858, USA.
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Skowronsky
authors.forename: Mechthild
authors.initials: M
authors.lastname: Schroeter
authors.forename: Tamatha
authors.initials: T
authors.lastname: Baxley
authors.forename: Yumin
authors.initials: Y
authors.lastname: Li
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Chalovich
authors.forename: Anne M
authors.initials: AM
authors.lastname: Spuches
mesh_terms: D001665:Binding Sites; D002118:Calcium / Q000378:metabolism*; D006801:Humans; D056004:Molecular Dynamics Simulation; D009206:Myocardium / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D013696:Temperature; D013816:Thermodynamics; D019209:Troponin C / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D010446:Peptide Fragments; D019209:Troponin C; D002118:Calcium