title: Identification of a novel equine infectious anemia virus field strain isolated from feral horses in southern Japan.
abstract: Although equine infectious anemia (EIA) was described more than 150 years ago, complete genomic sequences have only been obtained from two field strains of EIA virus (EIAV), EIAV(Wyoming) and EIAV(Liaoning). In 2011, EIA was detected within the distinctive feral Misaki horse population that inhabits the Toi-Cape area of southern Japan. Complete proviral sequences comprising a novel field strain were amplified directly from peripheral blood of one of these EIAV-infected horses and characterized by nucleotide sequencing. The complete provirus of Miyazaki2011-A strain is 8208 bp in length with an overall genomic organization typical of EIAV. However, this field isolate possesses just 77.2 and 78.7 % nucleotide sequence identity with the EIAV(Wyoming) and EIAV(Liaoning) strains, respectively, while similarity plot analysis suggested all three strains arose independently. Furthermore, phylogenetic studies using sequences obtained from all EIAV-infected Misaki horses against known viral strains strongly suggests these Japanese isolates comprise a separate monophyletic group.
authors.affiliation: The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753-8511, Japan.|Research Fellow DC of the Japan Society for the Promotion of Science, Tokyo 102-8472, Japan.|Departments of Veterinary Microbiology, University of Miyazaki, Miyazaki 889-2192, Japan.
authors.forename: Jian-Bao
authors.initials: JB
authors.lastname: Dong
authors.affiliation: Research Fellow DC of the Japan Society for the Promotion of Science, Tokyo 102-8472, Japan.|Departments of Veterinary Microbiology, University of Miyazaki, Miyazaki 889-2192, Japan.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY 40506, USA.
authors.forename: Frank R
authors.initials: FR
authors.lastname: Cook
authors.affiliation: Departments of Veterinary Microbiology, University of Miyazaki, Miyazaki 889-2192, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Goto
authors.affiliation: Veterinary Parasitic Diseases, University of Miyazaki, Miyazaki 889-2192, Japan.
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Horii
authors.affiliation: Division of Infection Control and Disease Prevention, Department of Veterinary Medical Science, University of Tokyo, Tokyo 113-8654, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Haga
mesh_terms: D000818:Animals; D001769:Blood / Q000821:virology; D016000:Cluster Analysis; D004279:DNA, Viral / Q000235:genetics*; D004859:Equine Infectious Anemia / Q000821:virology*; D016679:Genome, Viral; D006736:Horses; D004860:Infectious Anemia Virus, Equine / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D011533:Proviruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral