title: An improved method for analysis of glucosylceramide species having cis-8 and trans-8 isomers of sphingoid bases by LC-MS/MS.
abstract: High-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) approaches have enabled high selectivity and sensitivity for the identification and quantification of glucosylceramide molecular species. Here we demonstrate that HPLC-ESI-MS/MS is an efficient method for characterizing plant glucosylceramide species having the cis-8 and trans-8 isomers of sphingoid bases. Complete baseline separation was achieved using a high-carbon-content octadecylsilyl column and a simple binary gradient comprising methanol and water. The result of 2-hydroxy fatty acid composition achieved by HPLC-ESI-MS/MS was compared with that achieved by gas chromatography with flame ionization detection (GC-FID), indicating that the two methods yield similar molar compositions. The current method should be applicable to seeking the active components of glucosylceramide species from plant materials in response to biological challenges.
authors.affiliation: Department of Biology, Graduate School of Natural Science, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan. imai@konan-u.ac.jp
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Imai
authors.forename: Hideyasu
authors.initials: H
authors.lastname: Hattori
authors.forename: Masayuki
authors.initials: M
authors.lastname: Watanabe
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D005963:Glucosylceramides / Q000737:chemistry*; D007536:Isomerism; D012275:Oryza / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013110:Sphingosine / Q000737:chemistry
chemical_list: D005963:Glucosylceramides; D013110:Sphingosine