title: Detection of vim- and ipm-type metallo-beta-lactamases in Pseudomonas aeruginosa clinical isolates.
abstract: BACKGROUND
  Pseudomonas aeruginosa is the most important bacterium isolated from burn wounds, and its resistance to imipenem due to metallo-beta-lactamases is increasing. This study was designed to detect vim1, vim2, ipm1 and ipm2 metallo-beta-lactamases genes between Pseudomonas aeruginosa isolates isolated from Shahid Motahari Burns Hospital, Iran.
  
  
  METHODS
  To that end, we isolated 483 nonduplicate consecutive isolates of P. aeruginosa from burn infections; and after biochemical confirmation, we examined the imipenem susceptibility via the Kirby-Bauer method. All the imipenem-resistant and imipenem-intermediate isolates were screened for vim1, vim2, ipm1 and ipm2 genes through the PCR method.
  
  
  RESULTS
  From the 483 isolates, 272 (56%) and 63 (13%) isolates had resistant and intermediate zones in their imipenem antibiogram pattern, respectively. Fifty-four (16.1%), 7 (2.1%), 22 (6.6%), and 11 (3.3%) of the resistant and intermediate isolates had vim1, vim2, ipm1 and ipm2 genes in their PCR results, respectively.
  
  
  CONCLUSIONS
  MBL-mediated imipenem resistance in P. aeruginosa is a cause for concern in the treatment of infective burn patients. The rate of imipenem resistance due to MBL was increased dramatically and newer versions of MBL families were detected for the first time. These results suggest that an effective method should be provided to fight MBL production in clinical isolates.
authors.affiliation: Molecular Pathology Department, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Sepehriseresht
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Boroumand
authors.forename: Leila
authors.initials: L
authors.lastname: Pourgholi
authors.forename: Maryam
authors.initials: M
authors.lastname: Sotoudeh Anvari
authors.forename: Ehsan
authors.initials: E
authors.lastname: Habibi
authors.forename: Mahboubeh
authors.initials: M
authors.lastname: Sattarzadeh Tabrizi
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002056:Burns / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D008297:Male; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D015378:Imipenem; D001618:beta-Lactamases