title: Identification of the protein target of myelin-binding ligands by immunohistochemistry and biochemical analyses.
abstract: The ability to visualize myelin is important in the diagnosis of demyelinating disorders and the detection of myelin-containing nerves during surgery. The development of myelin-selective imaging agents requires that a defined target for these agents be identified and that a robust assay against the target be developed to allow for assessment of structure-activity relationships. We describe an immunohistochemical analysis and a fluorescence polarization binding assay using purified myelin basic protein (MBP) that provides quantitative evidence that MBP is the molecular binding partner of previously described myelin-selective fluorescent dyes such as BMB, GE3082, and GE3111.
authors.affiliation: GE Global Research, One Research Circle, Niskayuna, NY, USA.
authors.forename: Anshika
authors.initials: A
authors.lastname: Bajaj
authors.forename: Nicole E
authors.initials: NE
authors.lastname: LaPlante
authors.forename: Victoria E
authors.initials: VE
authors.lastname: Cotero
authors.forename: Kenneth M
authors.initials: KM
authors.lastname: Fish
authors.forename: Roger M
authors.initials: RM
authors.lastname: Bjerke
authors.forename: Tiberiu
authors.initials: T
authors.lastname: Siclovan
authors.forename: Cristina A
authors.initials: CA
authors.lastname: Tan Hehir
mesh_terms: D000814:Aniline Compounds; D000818:Animals; D003342:Corpus Striatum / Q000378:metabolism; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D008024:Ligands; D051379:Mice; D004676:Myelin Basic Protein / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D013267:Stilbenes; D013449:Sulfonamides; D014276:Trigeminal Nerve / Q000378:metabolism
chemical_list: C513133:1,4-bis(4-aminostyryl)-2-methoxybenzene; C578619:1-methylsulfonyl-4-(2-(4-(2-(4-aminophenyl)ethenyl)-3-methoxyphenyl)ethenyl)benzene; C578618:4-(2-(4-(2-(4-aminophenyl)ethenyl)-3-methoxyphenyl)ethenyl)benzonitrile; D000814:Aniline Compounds; D005456:Fluorescent Dyes; D008024:Ligands; D004676:Myelin Basic Protein; D013267:Stilbenes; D013449:Sulfonamides