title: [Frequency of minor histocompatibility antigens among Chilean blood donors].
abstract: BACKGROUND
  Minor histocompatibility antigens (mHAgs) play a critical role in the immune responses associated with allogeneic stem cell transplantation, such as graft versus host disease (GVHD) and graft-versus-tumor (GVT).
  
  
  OBJECTIVE
  To determine the gene frequencies of the mHAgs HA-1, HA-2 and HA-8 in Chilean Blood Bank donors.
  
  
  METHODS
  Blood from 192 blood donors was analyzed. The presence of haplotype HLA-A*02 was determined by flow cytometry. The frequency of mHAgs was determined by allele specific polymerase chain reaction in genomic DNA.
  
  
  RESULTS
  Sixty one participants were carriers of the haplotype HLA-A*02. The relative allele frequency HA-1H was 45%, HA-Ir 55%, HA-2V 80.6%, HA-2M 19.4%, HA-8R 49.8% and HA-8P was 50.2%. Based on mHAgs disparity between HA-1, HA-2 or HA-8, the probability to generate a GVT response in HLA-A*02 individuals was 40%.
  
  
  CONCLUSIONS
  The mHAgs frequency in Chilean population is under Hardy-Weinberg equilibrium and they are similar to those of other ethnic populations in the world.
authors.affiliation: Laboratorio de Terapia Celular, Banco de Sangre, Sangre Hospital Clínico Universidad de Chile, Chile.
authors.forename: Claudio
authors.initials: C
authors.lastname: Pérez N
authors.forename: Ramón
authors.initials: R
authors.lastname: Rabanales T
authors.forename: Milton
authors.initials: M
authors.lastname: Larrondo L
authors.forename: Jorge
authors.initials: J
authors.lastname: Alfaro L
mesh_terms: D001782:Blood Donors; D002677:Chile; D005787:Gene Frequency / Q000235:genetics*; D006086:Graft vs Host Disease / Q000235:genetics / Q000276:immunology; D020350:Graft vs Tumor Effect / Q000235:genetics; D006680:HLA Antigens / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D015778:Minor Histocompatibility Antigens / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction; D033581:Stem Cell Transplantation; D014184:Transplantation, Homologous
chemical_list: D006680:HLA Antigens; D015778:Minor Histocompatibility Antigens