title: Immunomagnetic nanoscreening of circulating tumor cells with a motion controlled microfluidic system.
abstract: Combining the power of immunomagnetic assay and microfluidic microchip operations, we successfully detected rare CTCs from clinical blood samples. The microfluidic system is operated in a flip-flop mode, where a computer-controlled rotational holder with an array of microfluidic chips inverts the microchannels. We have demonstrated both theoretically and experimentally that the direction of red blood cell (RBC) sedimentation with regards to the magnetic force required for cell separation is important for capture efficiency, throughput, and purity. The flip-flop operation reduces the stagnation of RBCs and non-specific binding on the capture surface by alternating the direction of the magnetic field with respect to gravity. The developed immunomagnetic microchip-based screening system exhibits high capture rates (more than 90%) for SkBr3, PC3, and Colo205 cell lines in spiked screening experiments and successfully isolates CTCs from patient blood samples. The proposed motion controlled microchip-based immunomagnetic system shows great promise as a clinical tool for cancer diagnosis and prognosis.
authors.affiliation: Department of Biomedical Engineering, The University of Texas at Austin, University Station, C0800, Austin, TX, 78712-0238, USA.
authors.forename: Yu-Yen
authors.initials: YY
authors.lastname: Huang
authors.affiliation: Department of Biomedical Engineering, The University of Texas at Austin, University Station, C0800, Austin, TX, 78712-0238, USA.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Hoshino
authors.affiliation: Department of Biomedical Engineering, The University of Texas at Austin, University Station, C0800, Austin, TX, 78712-0238, USA.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.affiliation: Department of Biomedical Engineering, The University of Texas at Austin, University Station, C0800, Austin, TX, 78712-0238, USA.
authors.forename: Chun-Hsien
authors.initials: CH
authors.lastname: Wu
authors.affiliation: Harold C. Simons Comprehensive Cancer Center of the University of Texas Southwestern, Medical Center, USA, 5323 Harry Hines Boulevard, Dallas, TX, 7390-852, USA.
authors.forename: Nancy
authors.initials: N
authors.lastname: Lane
authors.affiliation: Harold C. Simons Comprehensive Cancer Center of the University of Texas Southwestern, Medical Center, USA, 5323 Harry Hines Boulevard, Dallas, TX, 7390-852, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Huebschman
authors.affiliation: Harold C. Simons Comprehensive Cancer Center of the University of Texas Southwestern, Medical Center, USA, 5323 Harry Hines Boulevard, Dallas, TX, 7390-852, USA.
authors.forename: Huaying
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Imaging Physics, Division of Diagnostic Imaging, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX, 77030, USA.
authors.forename: Konstantin
authors.initials: K
authors.lastname: Sokolov
authors.affiliation: Harold C. Simons Comprehensive Cancer Center of the University of Texas Southwestern, Medical Center, USA, 5323 Harry Hines Boulevard, Dallas, TX, 7390-852, USA.
authors.forename: Jonathan W
authors.initials: JW
authors.lastname: Uhr
authors.affiliation: Harold C. Simons Comprehensive Cancer Center of the University of Texas Southwestern, Medical Center, USA, 5323 Harry Hines Boulevard, Dallas, TX, 7390-852, USA.
authors.forename: Eugene P
authors.initials: EP
authors.lastname: Frenkel
authors.affiliation: Department of Biomedical Engineering, The University of Texas at Austin, University Station, C0800, Austin, TX, 78712-0238, USA. john.zhang@engr.utexas.edu.
authors.forename: John X J
authors.initials: JXJ
authors.lastname: Zhang
mesh_terms: D001799:Blood Sedimentation; D045744:Cell Line, Tumor; D004912:Erythrocytes / Q000166:cytology; D006801:Humans; D018189:Immunomagnetic Separation / Q000295:instrumentation*; D060526:Magnetic Fields; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009038:Motion; D036103:Nanotechnology / Q000295:instrumentation*; D009369:Neoplasms / Q000097:blood; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D014783:Viscosity