title: Long term culture of cells patterned on glass via membrane-tethered oligonucleotides.
abstract: Oligonucleotide-based membrane inserts can be used as tethers to control attachment of cells to patterned surfaces without interfering with internal cytoskeletal modes of adhesion. Such control can be employed as a means for study of cell-cell interactions or side-by-side co-culture of different cell types without separation/sorting. While there is utility for cell patterning methods decoupled from natural cytoskeletal mechanisms, the consequences of maintaining this artificially induced state of attachment remains unexplored. We present a method for the 2-dimensional patterning of cells via hybridization of membrane-tethered single stranded oligonucleotides to complimentary single stranded oligonucleotides bound to optically transparent glass substrates which allowed us to characterize the long term culture of patterned HEK293 cells. Patterned substrates immersed in FBS-containing media are shown to permit the adsorption of adhesive serum proteins which allowed for the spreading and engagement of natural cytoskeletal adhesion modes in cells initially attached only through DNA hybridization. We show that the coexisting modes of attachment result in competition between membrane-bound tethers and natural cytoskeletal adhesion machinery as cells attempt to migrate away from their initial points of attachment. This competition ends in the escape of cells from their designated patterns and the 'winning out' of cytoskeletal migration forces over the affinity of lipid inserts for the cell membrane.
authors.affiliation: Department of Reparative Materials, Institute for Frontier Medical Sciences, Kyoto University, Sakyo-Ku, Kyoto, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Sakurai
authors.forename: Ian T
authors.initials: IT
authors.lastname: Hoffecker
authors.forename: Hiroo
authors.initials: H
authors.lastname: Iwata
mesh_terms: D001483:Base Sequence; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000032:analysis; D018929:Cell Culture Techniques / Q000379:methods*; D002465:Cell Movement; D049109:Cell Proliferation; D048430:Cell Shape; D004247:DNA / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry*; D020349:Surface Plasmon Resonance; D013499:Surface Properties
chemical_list: D015815:Cell Adhesion Molecules; D004277:DNA, Single-Stranded; D008081:Liposomes; D009841:Oligonucleotides; D004247:DNA