title: Global phylogeny of Shigella sonnei strains from limited single nucleotide polymorphisms (SNPs) and development of a rapid and cost-effective SNP-typing scheme for strain identification by high-resolution melting analysis.
abstract: The current Shigella sonnei pandemic involves geographically associated, multidrug-resistant clones. This study has demonstrated that S. sonnei phylogeny can be accurately defined with limited single nucleotide polymorphisms (SNPs). By typing 6 informative SNPs using a high-resolution melting (HRM) assay, major S. sonnei lineages/sublineages can be identified as defined by whole-genome variation.
authors.affiliation: Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom.
authors.forename: Vartul
authors.initials: V
authors.lastname: Sangal
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Holt
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Yuan
authors.forename: Derek J
authors.initials: DJ
authors.lastname: Brown
authors.forename: Ingrid
authors.initials: I
authors.lastname: Filliol-Toutain
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Weill
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Kim
authors.forename: Wanderley Dias
authors.initials: WD
authors.lastname: da Silveira
authors.forename: Derek
authors.initials: D
authors.lastname: Pickard
authors.forename: Nicholas R
authors.initials: NR
authors.lastname: Thomson
authors.forename: Julian
authors.initials: J
authors.lastname: Parkhill
authors.forename: Jun
authors.initials: J
authors.lastname: Yu
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D004405:Dysentery, Bacillary / Q000453:epidemiology / Q000382:microbiology; D014943:Global Health; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods; D058889:Molecular Typing / Q000379:methods*; D058873:Pandemics; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D012764:Shigella sonnei / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D044366:Transition Temperature