title: Verrucosispora fiedleri sp. nov., an actinomycete isolated from a fjord sediment which synthesizes proximicins.
abstract: A novel filamentous actinobacterial organism, designated strain MG-37(T), was isolated from a Norwegian fjord sediment and examined using a polyphasic taxonomic approach. The organism was determined to have chemotaxonomic and morphological properties consistent with its classification in the genus Verrucosispora and formed a distinct phyletic line in the Verrucosispora 16S rRNA gene tree. It was most closely related to Verrucosispora maris DSM 45365(T) (99.5 % 16S rRNA gene similarity) and Verrucosispora gifhornensis DSM 44337(T) (99.4 % 16S rRNA gene similarity) but was distinguished from these strains based on low levels of DNA:DNA relatedness (~56 and ~50 %, respectively). It was readily delineated from all of the type strains of Verrucosispora species based on a combination of phenotypic properties. Isolate MG-37(T) (=NCIMB 14794(T) = NRRL-B-24892(T)) should therefore be classified as the type strain of a novel species of Verrucosispora for which the name Verrucosispora fiedleri is proposed.
authors.affiliation: School of Biology, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK. michael.goodfellow@ncl.ac.uk
authors.forename: Michael
authors.initials: M
authors.lastname: Goodfellow
authors.forename: Roselyn
authors.initials: R
authors.lastname: Brown
authors.forename: Lina
authors.initials: L
authors.lastname: Ahmed
authors.forename: Wasu
authors.initials: W
authors.lastname: Pathom-Aree
authors.forename: Alan T
authors.initials: AT
authors.lastname: Bull
authors.forename: Amanda L
authors.initials: AL
authors.lastname: Jones
authors.forename: James E M
authors.initials: JE
authors.lastname: Stach
authors.forename: Tiago Domingues
authors.initials: TD
authors.lastname: Zucchi
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
mesh_terms: D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D063366:Estuaries; D019015:Geologic Sediments / Q000382:microbiology*; D008848:Micromonosporaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009664:Norway; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S