title: The role of the helper lipid dioleoylphosphatidylethanolamine (DOPE) for DNA transfection cooperating with a cationic lipid bearing ethylenediamine.
abstract: Gene therapy is expected to treat various incurable diseases including viral infections, autoimmune disorders, and cancers. Cationic lipids (CL) have been used as carriers of therapeutic DNAs for gene therapy because they can form a complex with DNA and such a complex can be incorporated into cells and transport the bound DNA to cytosol. The CL/DNA complexes are called lipoplexes and categorized as a non-viral vector. Lipoplexes are often prepared by adding a neutral phospholipid dioleoylphosphatidylethanolamine (DOPE) to CL in order to enhance transfection. However, the role of DOPE is not fully understood. We synthesized a new CL having an ethylenediamine cationic head group, denoted by DA, and found that addition of DOPE to DA achieved a good efficiency, almost in the similar level of commonly used transfection reagent Lipofectamine 2000 (Invitrogen). The composition of DA:DOPE=1:1 showed the highest efficiency. This lipoplex showed structural transition when pH was changed from 7 to 4, corresponding pH lowering in late endosome, while DOPE itself showed structural transition at more basic pH around 8. The present data showed that the DOPE/DA composition determines the structural transition pH and choosing a suitable pH, i.e., a suitable composition, is essential to increase the transfection efficiency.
authors.affiliation: Department of Chemistry and Biochemistry, The University of Kitakyushu, 1-1, Hibikino, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0135, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Mochizuki
authors.forename: Naho
authors.initials: N
authors.lastname: Kanegae
authors.forename: Koichi
authors.initials: K
authors.lastname: Nishina
authors.forename: Yumi
authors.initials: Y
authors.lastname: Kamikawa
authors.forename: Kazunori
authors.initials: K
authors.lastname: Koiwai
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Masunaga
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sakurai
mesh_terms: D001703:Biophysics / Q000379:methods; D002412:Cations; D004247:DNA / Q000737:chemistry; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism; D005029:Ethylenediamines / Q000737:chemistry*; D005786:Gene Expression Regulation; D015316:Genetic Therapy / Q000379:methods; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000737:chemistry; D008956:Models, Chemical; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D012542:Scattering, Radiation; D014162:Transfection; D014965:X-Rays
chemical_list: D002412:Cations; D005029:Ethylenediamines; D008055:Lipids; D010714:Phosphatidylethanolamines; D010743:Phospholipids; C094877:dioleoyl phosphatidylethanolamine; C031234:ethylenediamine; D004247:DNA