title: Phagocytosis and respiratory burst activity in lumpsucker (Cyclopterus lumpus L.) leucocytes analysed by flow cytometry.
abstract: In the present study, we have isolated leucocytes from peripheral blood, head kidney and spleen from lumpsucker (Cyclopterus lumpus L.), and performed functional studies like phagocytosis and respiratory burst, as well as morphological and cytochemical analyses. Different leucocytes were identified, such as lymphocytes, monocytes/macrophages and polymorphonuclear cells with bean shaped or bilobed nuclei. In addition, cells with similar morphology as described for dendritic cells in trout were abundant among the isolated leucocytes. Flow cytometry was successfully used for measuring phagocytosis and respiratory burst activity. The phagocytic capacity and ability were very high, and cells with different morphology in all three leucocyte preparations phagocytised beads rapidly. Due to lack of available cell markers, the identity of the phagocytic cells could not be determined. The potent non-specific phagocytosis was in accordance with a high number of cells positive for myeloperoxidase, an enzyme involved in oxygen-dependent killing mechanism present in phagocytic cells. Further, high respiratory burst activity was present in the leucocytes samples, verifying a potent oxygen- dependent degradation. At present, the specific antibody immune response could not be measured, as immunoglobulin or B-cells have not yet been isolated. Therefore, analyses of the specific immune response in this fish species await further clarification. The present study presents the first analyses of lumpsucker immunity and also the first within the order Scopaeniformes.
authors.affiliation: Department of Biology, University of Bergen, Bergen, Norway. gyri.haugland@bio.uib.no
authors.forename: Gyri T
authors.initials: GT
authors.lastname: Haugland
authors.forename: Ragnhild Aakre
authors.initials: RA
authors.lastname: Jakobsen
authors.forename: Nils
authors.initials: N
authors.lastname: Vestvik
authors.forename: Kristian
authors.initials: K
authors.lastname: Ulven
authors.forename: Lene
authors.initials: L
authors.lastname: Stokka
authors.forename: Heidrun I
authors.initials: HI
authors.lastname: Wergeland
mesh_terms: D000818:Animals; D002469:Cell Separation; D048429:Cell Size; D005399:Fishes / Q000097:blood / Q000276:immunology*; D005434:Flow Cytometry; D007668:Kidney / Q000166:cytology; D007962:Leukocytes / Q000166:cytology* / Q000201:enzymology / Q000276:immunology; D008297:Male; D010587:Phagocytosis; D016897:Respiratory Burst; D013154:Spleen / Q000166:cytology