title: [Effect of human placental extract on proliferation of human umbilical cord blood CD34(+) cells in vitro].
abstract: The study was aimed to investigate the hematopoietic function of placenta tissue and clarify the effect of human placental chorionic tissue in different periods on proliferation of hematopoietic stem cells in vitro, in order to further understand the changes of the hematopoietic function of placenta with the time prolonging. The experiments were divided into four groups: early placenta (group B), mid-term placenta (group C), full-term placenta (group D), and blank group (group A). The hematopoietic stem cells were amplified in co-culture way, and the colony formation ability after the expansion was observed. The results showed that compared to initial concentration, the CD34(+) cells cultured with full-term placenta were amplified by (2.60 ± 0.20) times, which was significantly higher than those CD34(+) cells cultured with mid-term placenta (1.74 ± 0.24) and early placenta (1.14 ± 0.12), but that in blank group was reduced without amplification. After culture for 14 days, the colony number of group C and group D were significantly higher than that of group A and group B. Among them the number of CFU-GM, CFU-GEMM, BFU-E of group C all were a little higher than that of group D. It is concluded that human placental extract in different period without any exogenous cell factors all can support the proliferation of hematopoietic stem cells, this ability is getting stronger with time increasing. The colony formation ability of the amplified cells shows weakened after the first increase, this colony formation ability of the amplified cells in group C is strongest, slightly stronger than that of group D.
authors.affiliation: Department of Paediatrics, Qianfoshan Hospital Affiliated to Shandong University, Shandong Province, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Ma
authors.forename: Hui
authors.initials: H
authors.lastname: Yao
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Guo
authors.forename: Lin
authors.initials: L
authors.lastname: Cheng
authors.forename: Jia-Hui
authors.initials: JH
authors.lastname: Li
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Liu
mesh_terms: D018952:Antigens, CD34; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D005312:Fetal Blood / Q000166:cytology* / Q000187:drug effects*; D006801:Humans; D010924:Placental Extracts / Q000494:pharmacology*
chemical_list: D018952:Antigens, CD34; D010924:Placental Extracts