title: Structural characterization of a modification subunit of a putative type I restriction enzyme from Vibrio vulnificus YJ016.
abstract: In multifunctional type I restriction enzymes, active methyltransferases (MTases) are constituted of methylation (HsdM) and specificity (HsdS) subunits. In this study, the crystal structure of a putative HsdM subunit from Vibrio vulnificus YJ016 (vvHsdM) was elucidated at a resolution of 1.80 Å. A cofactor-binding site for S-adenosyl-L-methionine (SAM, a methyl-group donor) is formed within the C-terminal domain of an α/β-fold, in which a number of residues are conserved, including the GxGG and (N/D)PP(F/Y) motifs, which are likely to interact with several functional moieties of the SAM methyl-group donor. Comparison with the N6 DNA MTase of Thermus aquaticus and other HsdM structures suggests that two aromatic rings (Phe199 and Phe312) in the motifs that are conserved among the HsdMs may sandwich both sides of the adenine ring of the recognition sequence so that a conserved Asn residue (Asn309) can interact with the N6 atom of the target adenine base (a methyl-group acceptor) and locate the target adenine base close to the transferred SAM methyl group.
authors.affiliation: Department of Chemistry, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Suk-Youl
authors.initials: SY
authors.lastname: Park
authors.forename: Hyun-Ju
authors.initials: HJ
authors.lastname: Lee
authors.forename: Jung-Mi
authors.initials: JM
authors.lastname: Song
authors.forename: Jiali
authors.initials: J
authors.lastname: Sun
authors.forename: Hyo-Jeong
authors.initials: HJ
authors.lastname: Hwang
authors.forename: Kosuke
authors.initials: K
authors.lastname: Nishi
authors.forename: Jeong-Sun
authors.initials: JS
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D018360:Crystallography, X-Ray; D015253:Deoxyribonucleases, Type I Site-Specific / Q000737:chemistry* / Q000378:metabolism; D008745:Methylation; D008780:Methyltransferases / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D012436:S-Adenosylmethionine / Q000378:metabolism; D016415:Sequence Alignment; D041261:Vibrio vulnificus / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism
chemical_list: D021122:Protein Subunits; D012436:S-Adenosylmethionine; D008780:Methyltransferases; D015253:Deoxyribonucleases, Type I Site-Specific