title: Evaluation of a rapid lateral flow immunoassay for Staphylococcus aureus detection in respiratory samples.
abstract: Rapid point-of-care pathogen detection remains a challenge in routine diagnostics. A Staphylococcus aureus-specific lateral flow immunochromatography (LFI) test has been developed using a specific monoclonal antibody to the S. aureus cell-wall peptidoglycan. The LFI test was shown to be specific for S. aureus with no signal development for other Staphylococcal species or common respiratory pathogens. Evaluation of S. aureus isolates spiked into induced sputum and bronchoalveolar lavage samples derived from severe asthmatic patients showed a detection limit of 10(6) CFU/mL for the LFI. The test was also shown to successfully detect S. aureus in 1 sample independently determined to be S. aureus positive by quantitative polymerase chain reaction. The ability of the LFI test to rapidly detect S. aureus in clinical respiratory samples suggests that it might be a useful platform for further development of point-of-care diagnostic applications.
authors.affiliation: Institute of Pharmaceutical Science, King's College London, London SE1 9NH, United Kingdom.
authors.forename: Surasa
authors.initials: S
authors.lastname: Wiriyachaiporn
authors.forename: Peter H
authors.initials: PH
authors.lastname: Howarth
authors.forename: Kenneth D
authors.initials: KD
authors.lastname: Bruce
authors.forename: Lea Ann
authors.initials: LA
authors.lastname: Dailey
mesh_terms: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001431:Bacteriological Techniques / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D006801:Humans; D019095:Point-of-Care Systems; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis*; D013211:Staphylococcus aureus / Q000276:immunology / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal