title: Bax is essential for death receptor-mediated apoptosis in human colon cancer cells.
abstract: To demonstrate the role of Bax in death receptor-induced apoptosis in the human colon cancer HCT116 cells. We treated HCT116 cells and HCT116 with p53(-/-) (KO) by 0.1 μg/mL TRAIL for 24 hours, which indicated that HCT116 parental cells are sensitive to p53-independent death receptor-induced apoptosis. Although the p53 signaling pathway is totally intact in this system, the down-regulation of Bax in HCT116 cells is dramatically resistant to TRAIL and failed to undergo apoptosis. However, the over-expression of Bax can rescue the sensitivity of apoptosis induced by the death receptor. Our study has revealed an essential role for Bax in death receptor-induced apoptosis in the human colon cancer HCT116 cells. It may aid in a molecular understanding of possible defects in signal transduction and a regulation of the death receptor-induced apoptotic process.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, Southwest University, Chongqing, China.
authors.forename: Shunqin
authors.initials: S
authors.lastname: Zhu
authors.forename: Tai
authors.initials: T
authors.lastname: Li
authors.forename: Juan
authors.initials: J
authors.lastname: Tan
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Yan
authors.forename: Dunke
authors.initials: D
authors.lastname: Zhang
authors.forename: Chunqin
authors.initials: C
authors.lastname: Zheng
authors.forename: Yibiao
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhonghuai
authors.initials: Z
authors.lastname: Xiang
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Cui
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D055786:Gene Knockout Techniques; D045325:HCT116 Cells; D006801:Humans; D053218:Receptors, Death Domain / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000494:pharmacology; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: C494826:BAX protein, human; D053218:Receptors, Death Domain; D053221:TNF-Related Apoptosis-Inducing Ligand; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein