title: UV-B Induced stimulation of phosphatidylinositol 3-kinase in human fetal hepatocytes.
abstract: We investigated the effect of UV-B irradiation on PI 3-kinase activity in human fetal hepatocytes. When cells were exposed to monochromatic (304nm) UV-B light, a significant increase in intracellular PI 3-kinase activity was observed in a dose dependent manner with maximal activity upon 1500 Jm(-2) irradiation. At 1500 Jm(-2) dose PI 3-kinse activity increased by 80% in membrane fraction of fetal hepatocytes of 25 weeks gestation. PI 3-kinse inhibitors wortmannin and LY294002 specifically inhibited the UV-B induced lipid kinase activity and blocked significantly the UV-B induced cell viability. The data suggests a correlation between cell survival and elevated levels of PI 3-kinase and suggest that UV-B irradiation at a dose of 1500 Jm(-2) is ideal for fetal hepatocyte transplantation. Also, PI 3-kinase levels could be a representative marker for viable UV-B irradiated fetal hepatocytes for transplantation.
authors.affiliation: Department of Biochemistry, Osmania University, Hyderabad.
authors.forename: V B
authors.initials: VB
authors.lastname: Chari
authors.forename: R S R
authors.initials: RS
authors.lastname: Gaddameedi
authors.forename: M Raghuveer
authors.initials: MR
authors.lastname: Singh
authors.forename: Sathish
authors.initials: S
authors.lastname: Kasina
authors.forename: Khaja Khaleel Ur
authors.initials: KK
authors.lastname: Rahman
authors.forename: C M
authors.initials: CM
authors.lastname: Habibullah
authors.forename: Surya S
authors.initials: SS
authors.lastname: Singh
keywords: Hepatocytes transplantation; Lipid kinase; Signal transduction; Xenotransplantation