title: Study on construction of cDNA library of the treated changliver cell and quality analysis.
abstract: The study aims to construct cDNA library of Changliver cell by SMART (switching mechanism at 5' end of RNA transcript) technique and analyze its quality. cDNA of Changliver cell was made with RT-PCR and LD-PCR (long-distance PCR), the cDNA library was constructed with SMART cDNA library construction kit. Through testing, the high quality cDNA library containing whole long cDNA of Changliver cell had been constructed. The titer of the amplified cDNA library was 4.5 × 10(10) pfu/ml and the average exogenous inserts of the recombinants is 1.5 kb. These results showed that the Changliver cell cDNA library had an excellent quality and lay foundation for screening whole long cDNA of related genes.
authors.affiliation: Department of Cell Biology, Xinxiang Medical College, 453003 Xinxiang, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Juntang
authors.forename: Jogenananda
authors.initials: J
authors.lastname: Pramanik
authors.forename: Wang
authors.initials: W
authors.lastname: Congrui
authors.forename: Zhang
authors.initials: Z
authors.lastname: Huiyong
authors.forename: Feng
authors.initials: F
authors.lastname: Huigen
authors.forename: Yang
authors.initials: Y
authors.lastname: Baosheng
authors.forename: Li
authors.initials: L
authors.lastname: Yuchang
authors.forename: Xu
authors.initials: X
authors.lastname: Cunshuan
keywords: Changliver cell; LD-PCR; SMART; cDNA library; immunoscreening