title: Simvastatin induces osteogenic differentiation in human amniotic fluid mesenchymal stem cells (AFMSC).
abstract: Amniotic fluid is a complex mixture composed of water, salts and different cells types derived from embryo exfoliation. Some of these cells present similar characteristics to mesenchymal stem cells as adherent properties, typical surface antigens and differentiation capacity. These cells are called amniotic fluid-derived mesenchymal stem cells (AFMSCs) and are easily obtained by amniocentesis, propagated in culture and differentiated in several cell types with specific inductions. In this study, we observe the ability of simvastatin, a 3-HMG-CoA reductase inhibitor, to induce AFSMCs osteogenic differentiation. When AFSMCs were incubated with medium containing simvastatin, it was observed morphological changes, calcium deposits formation confirmed by Alizarin Red stain. Differentiated cells also expressed typical osteogenic genes, as osteopontin and osteocalcin. In conclusion, simvastatin could be used as an optional osteogenic induction agent for amniotic fluid-derived mesenchymal stem cells.
authors.affiliation: Laboratório de Genética e Hematologia Molecular (LIM 31), Faculdade de Medicina da, Universidade de São Paulo, São Paulo, SP, Brazil; Universidade Bandeirante de São Paulo - Anhanguera Educacional, São Bernardo do Campo, SP, Brazil.
authors.forename: Felipe
authors.initials: F
authors.lastname: de Lara Janz
authors.forename: Giovani Marino
authors.initials: GM
authors.lastname: Favero
authors.forename: Milton Sérgio
authors.initials: MS
authors.lastname: Bohatch
authors.forename: Adrianade
authors.initials: A
authors.lastname: Aguiar Debes
authors.forename: Sergio Paulo
authors.initials: SP
authors.lastname: Bydlowski
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology*; D002118:Calcium / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015675:Osteocalcin / Q000235:genetics*; D053495:Osteopontin / Q000235:genetics*; D019821:Simvastatin / Q000494:pharmacology*
chemical_list: D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D015675:Osteocalcin; D053495:Osteopontin; D019821:Simvastatin; D002118:Calcium
keywords: amniotic stem cell; osteogenic differentiation; simvastatin