title: Serodiagnosis of Mycobacterium avium complex pulmonary disease in the USA.
abstract: Diagnosis of Mycobacterium avium complex pulmonary disease (MAC-PD) can be difficult. A previous study from Japan reported the usefulness of a serodiagnostic test for MAC-PD. The objective of this study was to evaluate the usefulness of the test in similar patients in the USA. 100 patients with known or suspected MAC-PD and 52 healthy volunteers were enrolled into the study at National Jewish Health, Denver, CO, USA. Serum glycopeptidolipid core immunoglobulin A antibody levels were measured with an enzyme immunoassay (EIA) kit and routine clinical evaluations were performed. The patients were divided into two groups based on clinical evaluation: 87 patients with MAC-PD that met American Thoracic Society criteria, and 13 who did not meet the criteria. The sensitivity and specificity (cut-off point 0.3 U·mL(-1)) of the serodiagnostic test for diagnosing MAC-PD were 70.1% and 93.9%, respectively. Among the 44 patients in the MAC-PD group with two or more positive sputum cultures within the previous 6 months, sensitivity was 81.8%. The EIA kit demonstrated good sensitivity and specificity for the identification of MAC-PD, particularly in patients with two or more positive cultures, and may be useful for rapid MAC-PD diagnosis.
authors.affiliation: National Hospital Organization, National Toneyama Hospital, Toyonaka-shi, Japan. kitadase@toneyama.go.jp
authors.forename: Seigo
authors.initials: S
authors.lastname: Kitada
authors.forename: Adrah
authors.initials: A
authors.lastname: Levin
authors.forename: Melissa
authors.initials: M
authors.lastname: Hiserote
authors.forename: Ron J
authors.initials: RJ
authors.lastname: Harbeck
authors.forename: Chris A
authors.initials: CA
authors.lastname: Czaja
authors.forename: Gwen
authors.initials: G
authors.lastname: Huitt
authors.forename: Shannon H
authors.initials: SH
authors.lastname: Kasperbauer
authors.forename: Charles L
authors.initials: CL
authors.lastname: Daley
mesh_terms: D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology; D003955:Diagnostic Tests, Routine; D005260:Female; D006017:Glycolipids / Q000097:blood; D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000097:blood; D008171:Lung Diseases / Q000453:epidemiology* / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D015269:Mycobacterium avium Complex / Q000302:isolation & purification*; D012372:ROC Curve; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods* / Q000592:standards; D014057:Tomography, X-Ray Computed; D014481:United States / Q000453:epidemiology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D006017:Glycolipids; D007070:Immunoglobulin A