title: Proliferation and differentiation of osteoblastic cells on silicon-doped TiO(2) film deposited by cathodic arc.
abstract: This study aimed at the proliferation and differentiation of osteoblastic cells on silicon-doped TiO(2) and pure TiO(2) films prepared by cathodic arc deposition. The films were examined by X-ray photo-electron spectroscopy, which showed that silicon was successfully doped into the Si-TiO(2) film. Meanwhile, no significant difference was found between the surface morphology of silicon-doped TiO(2) and pure TiO(2) films. When osteoblastic cells were cultured on silicon-doped TiO(2) film, accelerated cell proliferation was observed. Furthermore, cell differentiation was evaluated using alkaline phosphatase (ALP), type I collagen (COL I) and osteocalcin (OC) as differentiation markers. It was found that ALP activity, the expression levels of OC gene, COL I gene and protein were up-regulated on silicon-doped TiO(2) film at 3 and 5 days of culture. Moreover, no significant difference was found in apoptosis between the cells cultured on silicon-doped TiO(2) and pure TiO(2) films. Therefore, findings from this study indicate that silicon-doped film favors osteoblastic proliferation and differentiation, and has the potential for surface modification of implants in the future.
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, People's Republic of China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Junying
authors.initials: J
authors.lastname: Sun
authors.forename: Shi
authors.initials: S
authors.lastname: Qian
authors.forename: Xuanyong
authors.initials: X
authors.lastname: Liu
authors.forename: Shailin
authors.initials: S
authors.lastname: Zhang
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.forename: Shengjie
authors.initials: S
authors.lastname: Dong
authors.forename: Guochun
authors.initials: G
authors.lastname: Zha
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D020811:Biomedical Technology; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D024042:Collagen Type I / Q000235:genetics; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D015675:Osteocalcin / Q000235:genetics; D012825:Silicon / Q000737:chemistry / Q000494:pharmacology; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology
chemical_list: D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; D015675:Osteocalcin; C009495:titanium dioxide; D014025:Titanium; D000469:Alkaline Phosphatase; D012825:Silicon