title: A non-linear deterministic model for regulation of diauxic lag on cellobiose by the pneumococcal multidomain transcriptional regulator CelR.
abstract: When grown on glucose and beta-glucosides, S. pneumoniae shows sequential use of sugars resulting in diauxic growth with variable time extent of the lag phase separating the biphasic growth curve. The pneumococcal beta-glucoside uptake locus containing the PTS transporter spr0276-82, is regulated by a multi-domain transcriptional regulator CelR. In this work, we address the contribution of phosphorylation of the phosphorylable cysteine in the EIIB domain of CelR to diauxic lag. Utilising site-directed mutagenesis of the phosphorylable amino acids in the EIIB and EIIA domains of CelR, we show that the EIIB domain activation is linked to the duration of the lag phase. Analysis of mutants for other PTS systems indicates that a second beta-glucoside PTS (spr0505), not able to support growth on cellobiose, is responsible for the lag during diauxic growth. A mathematical model of the process is devised together with a nonlinear identification procedure which provides model parameter estimates characterizing the single phases of bacterial growth. Parameter identification performed on data recorded in appropriate experiments on mutants allows for establishing a relationship between a specific model parameter, the EIIB domain and the time extent of the diauxic lag. The experimental results and the related insights provided by the mathematical model provide evidence that the conflicting activation of the CelR regulator is at the origin of the lag phase during sequential growth on glucose and cellobiose. This data is the first description of diauxic lag regulation involving two PTS and a multidomain regulator and could serve as a promising approach for studying the S. pneumoniae growth process on complex carbon sources as possibly encountered in the human host.
authors.affiliation: Center for Complex Systems Studies (CSC), Department of Information Engineering, University of Siena, Siena, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Boianelli
authors.forename: Alessandro
authors.initials: A
authors.lastname: Bidossi
authors.forename: Luciana
authors.initials: L
authors.lastname: Gualdi
authors.forename: Laura
authors.initials: L
authors.lastname: Mulas
authors.forename: Chiara
authors.initials: C
authors.lastname: Mocenni
authors.forename: Gianni
authors.initials: G
authors.lastname: Pozzi
authors.forename: Antonio
authors.initials: A
authors.lastname: Vicino
authors.forename: Marco R
authors.initials: MR
authors.lastname: Oggioni
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002475:Cellobiose / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008962:Models, Theoretical; D010766:Phosphorylation; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013296:Streptococcus pneumoniae / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C119069:CelR protein, bacteria; D012097:Repressor Proteins; D002475:Cellobiose