title: Effect of insulin-like growth factor I on functional parameters of ram cooled-stored spermatozoa.
abstract: The aim of the study was to examine the effects of insulin-like growth factor I (IGF-I) on ram sperm traits after hypothermic storage. Sperm ejaculates from Lacaune rams were diluted in a Tris extender, pooled, divided into groups of IGF-I doses tested (0, 10, 100 or 200 ng.ml-1) and stored (0-5°C) for 96 h. IGF-I elevated whole sperm motility as measured by a Computer-assisted Sperm Analyser (CASA) system, by 24 h (10 ng.ml-1) and 48 h (200 ng.ml-1) of storage, and by progressive movement on each day of storage. After 72 h the sperm samples were analysed for plasma membrane integrity (peanut agglutinin-fluorescein isothiocyanate), membrane stability (annexin V-Fluos) and apoptosis (Yo-Pro®-1) using fluorescence microscopy. The addition of IGF-I (at 100 or 200 ng.ml-1) reduced the ratio of sperm with disrupted membranes and the ratio of annexin V-labelled sperm. The ratio of apoptotic sperm was reduced by IGF-I given at 10 or 100 ng.ml-1 compared with control. Sperm fertilizing ability, determined at 48 h by an in vitro fertilization (IVF) test on bovine oocytes, was increased by IGF-I given at 100 ng.ml-1 from 47.0 to 67.7%. In conclusion, IGF-I maintained ram sperm functions following cooling storage and its effects were reflected in sperm fertilizing ability in vitro.
authors.affiliation: Animal Production Research Centre Nitra,Hlohovecká 2,951 41 Lužianky near Nitra,Slovak Republic.
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Makarevich
authors.affiliation: Slovak Agricultural University,94901 Nitra,Slovak Republic.
authors.forename: Eliska
authors.initials: E
authors.lastname: Spalekova
authors.affiliation: Animal Production Research Centre Nitra,951 41 Lužianky near Nitra,Slovak Republic.
authors.forename: Lucia
authors.initials: L
authors.lastname: Olexikova
authors.affiliation: Animal Production Research Centre Nitra,951 41 Lužianky near Nitra,Slovak Republic.
authors.forename: Elena
authors.initials: E
authors.lastname: Kubovicova
authors.affiliation: Research Institute for Cattle Breeding Ltd. Rapotin,Vyzkumniku 267,788 13 Vikyrovice,Czech Republic.
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Hegedusova
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002417:Cattle; D002462:Cell Membrane / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005307:Fertilization in Vitro; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D008297:Male; D009865:Oocytes / Q000502:physiology; D012662:Semen Preservation / Q000379:methods; D012756:Sheep; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology*
chemical_list: D007334:Insulin-Like Growth Factor I