title: Fluorescent methods in the study of UV-induced changes in structural and functional state of human blood lymphocytes.
abstract: Structural and functional state of human blood lymphocytes after exposure to UV light (240-390 nm) in doses of 151-1359 J/m(2) was studied by methods of laser flow cytofluorometry, indirect immunofluorescence, and fluorescent probes. Using a combination of these methods, we have showed that UV light in the specified doses induced changes in the surface phenotype of T cells: stimulation or suppression of the expression of antigen-recognizing receptor complex molecules (CD3, CD4, and CD8 markers) and their redistribution on the surface of immunocompetent cells (capping effect) with the formation of receptor clusters of various types.
authors.affiliation: Voronezh State University, Voronezh, Russia. avg@main.vsu.ru
authors.forename: V G
authors.initials: VG
authors.lastname: Artyukhov
authors.forename: O V
authors.initials: OV
authors.lastname: Putintseva
authors.forename: V A
authors.initials: VA
authors.lastname: Vdovina
authors.forename: M V
authors.initials: MV
authors.lastname: Pashkov
authors.forename: D V
authors.initials: DV
authors.lastname: Vasilenko
mesh_terms: D000817:Anilino Naphthalenesulfonates; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000528:radiation effects*; D017252:CD3 Complex / Q000235:genetics* / Q000276:immunology; D015704:CD4 Antigens / Q000235:genetics* / Q000276:immunology; D016827:CD8 Antigens / Q000235:genetics* / Q000276:immunology; D002462:Cell Membrane / Q000378:metabolism / Q000528:radiation effects*; D002478:Cells, Cultured; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes; D015870:Gene Expression / Q000528:radiation effects; D006801:Humans; D013050:Spectrometry, Fluorescence; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D000817:Anilino Naphthalenesulfonates; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D005456:Fluorescent Dyes; C027132:1-anilino-8-naphthalenesulfonate