title: Acanthus ebracteatus Vahl. ethanol extract enhancement of the efficacy of the collagen scaffold in wound closure: a study in a full-thickness-wound mouse model.
abstract: Acanthus ebracteatus Vahl. is a Thai herb that is effective in wound healing. We sought to quantitatively determine whether or not the combined application of Acanthus ebracteatus Vahl. and a collagen scaffold will increase wound closure and angiogenesis. Balb/c mice (body weight: 22-25 g) were anesthetized with sodium thiopental. The dorsal skin incision measuring 1.5 × 1.5 cm was made and then deepened using scissors to produce a full-thickness incision down to the level of the panniculus carnosus. The size of the wound was approximately 10% of the total body surface area. The collagen sheet was implanted onto the wound. Animals were divided into 4 major groups as follows: wound with normal saline (W-NSS), wound treated with 0.3 g/kg BW of Acanthus ebracteatus Vahl. extract (W-AE (0.3 g/kg.bw)), wound implanted with collagen scaffold (W-Coll), and wound implanted with collagen scaffold and treated with 0.3 g/kg BW of Acanthus ebracteatus Vahl. (W-Coll-AE combination). On day 14, the W-Coll-AE group showed decreased wound areas and increased capillary vascularity (CV) when compared to the other 3 groups, W-NSS, W-AE0.3, and W-Coll. In the present study, the combination of AE0.3 with collagen showed the best effect on skin angiogenesis and promoted wound closure with less neutrophil infiltration.
authors.affiliation: Inter-Department of Physiology, Graduate School and Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Jutamas
authors.initials: J
authors.lastname: Somchaichana
authors.forename: Tanom
authors.initials: T
authors.lastname: Bunaprasert
authors.forename: Suthiluk
authors.initials: S
authors.lastname: Patumraj
mesh_terms: D027901:Acanthaceae / Q000737:chemistry*; D000818:Animals; D001458:Bandages; D003094:Collagen / Q000627:therapeutic use*; D003131:Combined Modality Therapy; D000431:Ethanol / Q000737:chemistry; D059625:Liquid-Liquid Extraction / Q000379:methods; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000627:therapeutic use*; D012867:Skin / Q000187:drug effects / Q000293:injuries; D054457:Tissue Scaffolds; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*; D014950:Wounds, Penetrating / Q000175:diagnosis / Q000628:therapy*
chemical_list: D010936:Plant Extracts; D000431:Ethanol; D003094:Collagen