title: Synthesis of PEGylated gold nanostars and bipyramids for intracellular uptake.
abstract: A great number of works have focused their research on the synthesis, design and optical properties of gold nanoparticles for potential biological applications (bioimaging, biosensing). For this kind of application, sharp gold nanostructures appear to exhibit the more interesting features since their surface plasmon bands are very sensitive to the surrounding medium. In this paper, a complete study of PEGylated gold nanostars and PEGylated bipyramidal-like nanostructures is presented. The nanoparticles are prepared in high yield and their surfaces are covered with a biocompatible polymer. The photophysical properties of gold bipyramids and nanostars, in suspension, are correlated with the optical response of single and isolated objects. The resulting spectra of isolated gold nanoparticles are subsequently correlated to their geometrical structure by transmission electron microscopy. Finally, the PEGylated gold nanoparticles were incubated with melanoma B16-F10 cells. Dark-field microscopy showed that the biocompatible gold nanoparticles were easily internalized and most of them localized within the cells.
authors.affiliation: Université de Lyon, ENS Lyon, CNRS, Université Lyon 1, Laboratoire de Chimie UMR 5182, 46, allée d'Italie, F-69364, Lyon cedex 07, France.
authors.forename: Julien R G
authors.initials: JR
authors.lastname: Navarro
authors.forename: Delphine
authors.initials: D
authors.lastname: Manchon
authors.forename: Frédéric
authors.initials: F
authors.lastname: Lerouge
authors.forename: Nicholas P
authors.initials: NP
authors.lastname: Blanchard
authors.forename: Sophie
authors.initials: S
authors.lastname: Marotte
authors.forename: Yann
authors.initials: Y
authors.lastname: Leverrier
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Marvel
authors.forename: Frédéric
authors.initials: F
authors.lastname: Chaput
authors.forename: Guillaume
authors.initials: G
authors.lastname: Micouin
authors.forename: Ana-Maria
authors.initials: AM
authors.lastname: Gabudean
authors.forename: Alexis
authors.initials: A
authors.lastname: Mosset
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Cottancin
authors.forename: Patrice L
authors.initials: PL
authors.lastname: Baldeck
authors.forename: Kenji
authors.initials: K
authors.lastname: Kamada
authors.forename: Stephane
authors.initials: S
authors.lastname: Parola
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D004705:Endocytosis; D006046:Gold / Q000737:chemistry* / Q000493:pharmacokinetics; D006651:Histocytochemistry; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D002593:Cetrimonium Compounds; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; D011092:Polyethylene Glycols; C007556:domiphen; D006046:Gold; D000077286:Cetrimonium