title: Optically absorbing nanoparticle mediated cell membrane permeabilization.
abstract: Membrane permeabilization is imperative for gene and drug delivery systems, along with other cell manipulation methods, since the average eukaryotic cell membrane is not permeable to polar and large nonpolar molecules. Antibody conjugated optically absorbing gold nanospheres are targeted to the cell membrane of T47D breast cancer cell line and irradiated with 5 ns pulse, 20 Hz, 532 nm light to increase membrane permeability. Up to 90% permeabilization with less than 6% death is reported at radiant exposures up to 10 times lower than those of other comparable studies.
authors.affiliation: Department of Biological Engineering, University of Missouri, Columbia, Missouri 65201, USA.
authors.forename: Kiran
authors.initials: K
authors.lastname: Bhattacharyya
authors.forename: Smit
authors.initials: S
authors.lastname: Mehta
authors.forename: John
authors.initials: J
authors.lastname: Viator
mesh_terms: D000042:Absorption; D045744:Cell Line, Tumor; D002463:Cell Membrane Permeability / Q000528:radiation effects; D006046:Gold / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D007834:Lasers; D053768:Metal Nanoparticles; D055620:Optical Phenomena
chemical_list: D006046:Gold