title: Sequence Analysis of the Second Internal Transcribed Spacer (ITS2) Region of rDNA for Species Identification of Trichostrongylus Nematodes Isolated From Domestic Livestock in Iran.
abstract: BACKGROUND
  Infectivity of herbivores with Trichostrongylus nematodes is widespread in many countries, having a major economic impact on breeding, survivability, and productivity of domestic livestock. This study was carried out on Trichostrongylus species isolated from domestic livestock in order to develop an easy-to-perform method for species identification.
  
  
  METHODS
  Trichostrongylus isolates were collected from sheep, goat, cattle, and buffaloes in Khuzestan Province, southwest Iran. Primary species identification was carried out based on morphological characterization of male worms. PCR amplification of ITS2-rDNA region was performed on genomic DNA and the products were sequenced. Phylogenetic analysis of the nucleotide sequence data was conducted employing Bayesian Inference approach. Consequently, a restriction fragment length polymorphism (RFLP) profile was designed to differentiate Trichostrongylus species.
  
  
  RESULTS
  A consensus sequence of 238 nucleotides was deposited in the GenBank for Iranian isolates of Trichostrongylus species including T. colubriformis, T. capricola, T. probolurus and T. vitrinus. The designated RFLP using restriction enzyme TasI could readily differentiate among species having different ITS2 sequence. The molecular analysis was in concordance with morphological findings.
  
  
  CONCLUSIONS
  Phylogenetic analysis indicated a close relationship among the sequences obtained in this study and reference sequence of relevant species. ITS2-RFLP with TasI is recommended for molecular differentiation of common Trichostrongylus species.
authors.affiliation: Vali-e-asr Hospital, Arak University of Medical Sciences, Arak, Iran ; Department of Medical Parasitology & Mycology, School of Public Health; National Institute of Health Research, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: R
authors.initials: R
authors.lastname: Ghasemikhah
authors.forename: M
authors.initials: M
authors.lastname: Sharbatkhori
authors.forename: I
authors.initials: I
authors.lastname: Mobedi
authors.forename: Eb
authors.initials: E
authors.lastname: Kia
authors.forename: M Fasihi
authors.initials: MF
authors.lastname: Harandi
authors.forename: H
authors.initials: H
authors.lastname: Mirhendi
keywords: ITS2; Iran; Livestock; Trichostrongylus