title: Drosophila miR-5 suppresses Hedgehog signaling by directly targeting Smoothened.
abstract: Hedgehog (Hh) signaling plays many important roles in developmental processes and cancers. Smoothened (Smo) is an important signal transducer in the Hh pathway, and its expression is tightly regulated by several different post-transcriptional mechanisms. However, whether microRNAs (miRNAs) are involved in Smo regulation is still unclear. Here, we found that miR-5 acts as a suppressor of the Hh pathway by targeting Smo. Through in vivo sensor assay and in vitro luciferase assay, we found that miR-5 downregulates Smo through directly binding to its 3'UTR. Moreover, our data indicated Costal-2 (Cos2) and Fused (Fu) do not play a role in the reduction of Smo mediated by miR-5. Furthermore, we determined that miR-5 not involved in Notch or Dpp signaling pathways by detecting target gene expression. Together, our results indicate that miR-5 can specifically suppress Hh signaling by directly targeting Smo in Drosophila.
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
authors.forename: Long-Fei
authors.initials: LF
authors.lastname: Wu
authors.forename: Lei
authors.initials: L
authors.lastname: Gao
authors.forename: Xiao-Meng
authors.initials: XM
authors.lastname: Hou
authors.forename: Qing-Hai
authors.initials: QH
authors.lastname: Zhang
authors.forename: Shen
authors.initials: S
authors.lastname: Li
authors.forename: Yong-Fei
authors.initials: YF
authors.lastname: Yang
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Lin
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D015536:Down-Regulation; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D053823:Hedgehog Proteins / Q000235:genetics* / Q000378:metabolism; D060227:Imaginal Discs / Q000254:growth & development / Q000378:metabolism; D007150:Immunohistochemistry; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D015398:Signal Transduction / Q000235:genetics*; D000072086:Smoothened Receptor; D014921:Wings, Animal / Q000254:growth & development / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D029721:Drosophila Proteins; D053823:Hedgehog Proteins; C577978:MIRN5 microRNA, Drosophila; D035683:MicroRNAs; D043562:Receptors, G-Protein-Coupled; D000072086:Smoothened Receptor; C100664:smo protein, Drosophila; D049452:Green Fluorescent Proteins; C077245:hh protein, Drosophila