title: Monoclonal and polyclonal antibodies specific for foot and mouth disease virus type A and type O VP1.
abstract: The foot and mouth disease virus (FMDV) is an RNA virus composed of single stranded positive sense RNA. FMDV has been known to infect cloven-hoofed animals, including pigs, cattle, and sheep. FMDV is rapidly spreading outward to neighboring regions, often leading to a high mortality rate. Thus, early diagnosis of FMDV is critical to suppress propagation of FMDV and minimize economic losses. In this study, we report the generation and characterization of polyclonal and six monoclonal antibodies against VP1 through immunoblotting and immunofluorescence microscopy analyses. These VP1 antibodies will be useful as tools to detect serotypes A and O of FMDVs for diagnostic usage.
authors.affiliation: Laboratory for Tracing of Gene Function, Department of Biomedical Science, CHA University, Bundang-gu, Sungnam-si, Gyunggido, Korea.
authors.forename: Jin Gu
authors.initials: JG
authors.lastname: Cho
authors.forename: Yeong Joon
authors.initials: YJ
authors.lastname: Jo
authors.forename: Jong-Hyuk
authors.initials: JH
authors.lastname: Sung
authors.forename: Jang-Kwan
authors.initials: JK
authors.lastname: Hong
authors.forename: Ji-Hyeon
authors.initials: JH
authors.lastname: Hwang
authors.forename: Jong-Hyeon
authors.initials: JH
authors.lastname: Park
authors.forename: Kwang-Nyeong
authors.initials: KN
authors.lastname: Lee
authors.forename: Sang Gyu
authors.initials: SG
authors.lastname: Park
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D001666:Binding Sites, Antibody; D015153:Blotting, Western; D036022:Capsid Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D029544:Foot-and-Mouth Disease Virus / Q000737:chemistry / Q000276:immunology*; D015870:Gene Expression; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D011817:Rabbits; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D012703:Serotyping
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D011994:Recombinant Proteins; C442501:VP1 protein, Foot-and-mouth disease virus