title: Lack of association between Chlamydia trachomatis infection and cervical cancer--Taq Man realtime PCR assay findings.
abstract: BACKGROUND
  Cervical cancer is one of the most common cancers in developing countries and the second most common type of cancer in women globally. Several recent studies suggested a co factor role for Chlamydia trachomatis in pathogenesis of cervical cancer. This study aimed to evaluate existence of C. trachomatis DNA in pathologic blocks of patients with cervical cancer.
  
  
  METHODS
  Seventy-six formaldehyde fixed paraffin embedded tissue specimens from patients with histologically proven history of cervical cancer as well as 150 blocks from healthy peoples were included in the present study. Thin slices were prepared from selected blocks followed by deparaffinization and DNA extraction; the presence of C. trachomatis DNA was examined by Taq Man real-time PCR.
  
  
  RESULTS
  Our TaqMan real time PCR assay with cervical specimens of patients with cervical cancer showed that there was no C. trachomatis DNA. Also, we found three positive specimens among our control group.
  
  
  CONCLUSIONS
  It seems that based on results obtained from the specimens examined in the present study, there is no association between the presence of C. trachomatis DNA in cervical specimens and cervical cancer.
authors.affiliation: Cell and Molecular Research Center, Qazvin University of Medical Sciences, Iran. tnaserpour@qums.ac.ir
authors.forename: Taghi Naserpour
authors.initials: TN
authors.lastname: Farivar
authors.forename: Pouran
authors.initials: P
authors.lastname: Johari
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000209:etiology*; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002584:Cervix Uteri / Q000378:metabolism; D002690:Chlamydia Infections / Q000150:complications* / Q000235:genetics / Q000821:virology; D002692:Chlamydia trachomatis / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D006801:Humans; D008875:Middle Aged; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000209:etiology*; D055815:Young Adult
chemical_list: D004279:DNA, Viral