title: Combining the physical adsorption approach and the covalent attachment method to prepare a bifunctional bioreactor.
abstract: Aminopropyl-functionalized SBA-15 mesoporous silica was used as a support to adsorb myoglobin. Then, in order to avoid the leakage of adsorbed myoglobin, lysozyme was covalently tethered to the internal and external surface of the mesoporous silica with glutaraldehyde as the coupling agent. The property of amino-functionalized mesoporous silica was characterized by N(2) adsorption-desorption and thermogravimetric (TG) analysis. The feature of the silica-based matrix before and after myoglobin adsorption was identified by fourier transform infrared (FTIR) and UV/VIS measurement. With o-dianisidine and H(2)O(2) as the substrate, the peroxidase activity of adsorbed myoglobin was determined. With Micrococus lysodeilicus as the substrate, the antibacterial activity of covalently tethered lysozyme was measured. Results demonstrated that the final product not only presented peroxidase activity of the myoglobin but yielded antibacterial activity of the lysozyme.
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, China.
authors.forename: Mengxing
authors.initials: M
authors.lastname: Dong
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, China.
authors.forename: Zhuofu
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Lu
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, China.
authors.forename: Zhengqiang
authors.initials: Z
authors.lastname: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism*; D019149:Bioreactors; D003962:Dianisidine / Q000378:metabolism*; D005976:Glutaral / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008837:Micrococcus / Q000378:metabolism*; D009113:Muramidase / Q000737:chemistry; D009211:Myoglobin / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism; D012822:Silicon Dioxide / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000900:Anti-Bacterial Agents; D009211:Myoglobin; C509969:SBA-15; D012822:Silicon Dioxide; D006861:Hydrogen Peroxide; D010544:Peroxidases; D009113:Muramidase; D003962:Dianisidine; D005976:Glutaral
keywords: adsorption; amino-functionalized mesoporous silica; antibacterial activity; covalent attachment; lysozyme; myoglobin; peroxidase activity