title: Genetic diversity characterization of porcine reproductive and respiratory syndrome virus isolates in Romania, based on phylogenetic analysis.
abstract: Porcine reproductive and respiratory syndrome (PRRS) is a disease produced by the (PRRS) virus, characterized by endemic evolution in the majority of countries, which remains in actuality being a permanent threat to health and economic free farms, as well as for those infected. The aim of this study was to evaluate the genetic diversity of Romanian PRRSV isolates from the four most important pig farms in Romania by comparing the nucleotide sequences obtained for ORF5 and ORF7 with a wide range of sequences from GenBank belonging to the main types of PRRSV; the type 1. Eighteen different sequences were obtained for ORF5 gene and 10 for ORF7 gene. One Romanian isolate (Rom3) was found in three of the four different investigated farms. The phylogenetic analysis revealed that the Romanian PRRSV nucleotide sequences clustered in three groups within the subtype 1 of the virus. The analysis of amino acid sequences evidenced for GP5 and N-nucleocapsid proteins confirmed that the Romanian virus belonged to type 1.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, 5th district Bucharest, Romania.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Zaulet
authors.affiliation: Faculty of Veterinary Medicine, University of Agronomical Science and Veterinary Medicine Bucharest, 105 Splaiul Independentei, 5th district Bucharest, Romania.
authors.forename: Maria Rodica
authors.initials: MR
authors.lastname: Gurau
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, 5th district Bucharest, Romania.
authors.forename: Vlad
authors.initials: V
authors.lastname: Petrovan
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, 5th district Bucharest, Romania.
authors.forename: Laura
authors.initials: L
authors.lastname: Buburuzan
mesh_terms: D000818:Animals; D014644:Genetic Variation; D016366:Open Reading Frames; D010802:Phylogeny; D019316:Porcine respiratory and reproductive syndrome virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012383:Romania; D013552:Swine
keywords: ORF5; ORF7; phylogeny; porcine reproductive and respiratory virus; swine