title: [Effects of matrine on the proliferation and apoptosis of human rhabdomyosarcoma RD cells].
abstract: OBJECTIVE
  To investigate the effects of matrine on the proliferation and apoptosis of human rhabdomyosarcoma RD cells in vitro, and to explore the mechanism of matrine inducing apoptosis of RD cells.
  
  
  METHODS
  MTT assay was used to measure the proliferation inhibition rates of RD cells that were treated with matrine (final concentrations= 0.5, 1.0, 1.5, and 2.0 mg/mL). Flow cytometry was used to evaluate the apoptosis of RD cells treated with the four concentrations of matrine. RT-PCR was used to measure the mRNA expression of cyclin D1 and survivin in RD cells treated with 0.5, 1.0, and 1.5 mg/mL of matrine.
  
  
  RESULTS
  The RD cells treated with various concentrations of matrine showed significantly higher proliferation inhibition rates and apoptotic rates than those that were not treated with matrine (P<0.01), and with increased matrine concentration, the proliferation inhibition rate of RD cells increased gradually, thus exhibiting a dose dependence. The mRNA expression of cyclin D1 and survivin was seen in all RD cells, but was significantly lower in RD cells treated with matrine than in those that were not treated with matrine (P<0.01). There were significant differences in cyclin D1 mRNA level among the RD cells treated with 0.5, 1.0, and 1.5 mg/mL of matrine (P<0.05), while there was significant difference in survivin mRNA level between the RD cells treated with 0.5 and 1.5 mg/mL of matrine (P<0.05).
  
  
  CONCLUSIONS
  Matrine can significantly inhibit proliferation and induce apoptosis of RD cells, which may be related to downregulating the mRNA expression of cyclin D1 and survivin.
authors.affiliation: Department of Pediatrics, The Hospital Affiliated to Xuzhou Medical College, Xuzhou, Jiangsu 221002, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Guo
authors.forename: Tian-Yang
authors.initials: TY
authors.lastname: Xue
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Ji-Zhao
authors.initials: JZ
authors.lastname: Gao
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D019938:Cyclin D1 / Q000235:genetics; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics; D011807:Quinolizines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D012208:Rhabdomyosarcoma / Q000188:drug therapy* / Q000473:pathology; D000077022:Survivin; D000093842:Matrines
chemical_list: D000470:Alkaloids; C107489:BIRC5 protein, human; C530104:CCND1 protein, human; D051034:Inhibitor of Apoptosis Proteins; D011807:Quinolizines; D012333:RNA, Messenger; D000077022:Survivin; D019938:Cyclin D1; D000093842:Matrines