title: Temporal and spatial expression of KIF3B after acute spinal cord injury in adult rats.
abstract: The KIF3 subunit KIF3B was proved to be associated with mitosis. It has been known to be engaged in intracellular transport of neurons. To elucidate the certain expression and biological function in central nervous system, we performed an acute spinal cord contusion injury model in adult rats. Western blot analysis indicated a marked upregulation of KIF3B after spinal cord injury (SCI). Immunohistochemistry revealed wide distribution of KIF3B in spinal cord, including neurons and glial cells. Double immunofluorescent staining for proliferating cell nuclear antigen and phenotype-specific markers showed increases of KIF3B expression in proliferating microglia and astrocytes. Our data suggest that KIF3B may be implicated in the proliferation of microglia and astrocytes after SCI.
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Yu
authors.forename: Hai
authors.initials: H
authors.lastname: Wen
authors.forename: Jianhua
authors.initials: J
authors.lastname: Cao
authors.forename: Binbin
authors.initials: B
authors.lastname: Sun
authors.forename: Tao
authors.initials: T
authors.lastname: Ding
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Long
authors.initials: L
authors.lastname: Long
authors.forename: Xinghai
authors.initials: X
authors.lastname: Cheng
authors.forename: Guangfei
authors.initials: G
authors.lastname: Xu
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D015870:Gene Expression; D016547:Kinesins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000378:metabolism*
chemical_list: D018809:Proliferating Cell Nuclear Antigen; C577559:Kif3B protein, rat; D016547:Kinesins