title: What you always needed to know about electroporation based DNA vaccines.
abstract: Vaccinations are increasingly used to fight infectious disease, and DNA vaccines offer considerable advantages, including broader possibilities for vaccination and lack of need for cold storage. It has been amply demonstrated, that electroporation augments uptake of DNA in both skin and muscle, and it is foreseen that future DNA vaccination may to a large extent be coupled with and dependent upon electroporation based delivery. Understanding the basic science of electroporation and exploiting knowledge obtained on optimization of DNA electrotransfer to muscle and skin, may greatly augment efforts on vaccine development. The purpose of this review is to give a succinct but comprehensive overview of electroporation as a delivery modality including electrotransfer to skin and muscle. As well, this review will speculate and discuss future uses for this powerful electrotransfer technology.
authors.affiliation: Center for Experimental Drug and Gene Electrotransfer (C*EDGE), Department of Oncology, Copenhagen University Hospital Herlev, Herlev, Denmark.
authors.forename: Anita
authors.initials: A
authors.lastname: Gothelf
authors.forename: Julie
authors.initials: J
authors.lastname: Gehl
mesh_terms: D000818:Animals; D018274:Electroporation; D006801:Humans; D009132:Muscles / Q000378:metabolism; D012867:Skin / Q000378:metabolism; D019444:Vaccines, DNA / Q000008:administration & dosage*
chemical_list: D019444:Vaccines, DNA
keywords: DNA vaccine; electroporation; gene electrotransfer; gene therapy; gene transfection; muscle; skin