title: Microbial diversity in long-term water-flooded oil reservoirs with different in situ temperatures in China.
abstract: Water-flooded oil reservoirs have specific ecological environments due to continual water injection and oil production and water recycling. Using 16S rRNA gene clone library analysis, the microbial communities present in injected waters and produced waters from four typical water-flooded oil reservoirs with different in situ temperatures of 25 °C, 40 °C, 55 °C and 70 °C were examined. The results obtained showed that the higher the in situ temperatures of the oil reservoirs is, the less the effects of microorganisms in the injected waters on microbial community compositions in the produced waters is. In addition, microbes inhabiting in the produced waters of the four water-flooded oil reservoirs were varied but all dominated by Proteobacteria. Moreover, most of the detected microbes were not identified as indigenous. The objective of this study was to expand the pictures of the microbial ecosystem of water-flooded oil reservoirs.
authors.affiliation: The Key Laboratory of Marine Reservoir Evolution and Hydrocarbon Accumulation Mechanism, Ministry of Education, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.forename: Yue-Hui
authors.initials: YH
authors.lastname: She
authors.forename: Lu-Jun
authors.initials: LJ
authors.lastname: Chai
authors.forename: Ibrahim M
authors.initials: IM
authors.lastname: Banat
authors.forename: Xiao-Tao
authors.initials: XT
authors.lastname: Zhang
authors.forename: Fu-Chang
authors.initials: FC
authors.lastname: Shu
authors.forename: Zheng-Liang
authors.initials: ZL
authors.lastname: Wang
authors.forename: Long-Jiang
authors.initials: LJ
authors.lastname: Yu
authors.forename: Du-Jie
authors.initials: DJ
authors.lastname: Hou
mesh_terms: D044822:Biodiversity; D002681:China; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D017753:Ecosystem; D005648:Fuel Oils / Q000382:microbiology* / Q000633:toxicity; D010802:Phylogeny; D020560:Proteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D013696:Temperature; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D004269:DNA, Bacterial; D005648:Fuel Oils; D014874:Water Pollutants, Chemical