title: Oral T4-like phage cocktail application to healthy adult volunteers from Bangladesh.
abstract: The genomic diversity of 99 T4-like coliphages was investigated by sequencing an equimolar mixture with Illumina technology and screening them against different databases for horizontal gene transfer and undesired genes. A 9-phage cocktail was given to 15 healthy adults from Bangladesh at a dose of 3×10(9) and 3×10(7) plaque-forming units and placebo respectively. Phages were detected in 64% of the stool samples when subjects were treated with higher titer phage, compared to 30% and 28% with lower-titer phage and placebo, respectively. No Escherichia coli was present in initial stool samples, and no amplification of phage was observed. One percent of the administered oral phage was recovered from the feces. No adverse events were observed by self-report, clinical examination, or from laboratory tests for liver, kidney, and hematology function. No impact of oral phage was seen on the fecal microbiota composition with respect to bacterial 16S rRNA from stool.
authors.affiliation: International Centre for Diarrhoeal Diseases Research, Bangladesh (icddr,b), 68 Shaheed Tajuddin Ahmed Sharani, Mohakhali, Dhaka 1212, Bangladesh. sasarker@icddrb.org
authors.forename: Shafiqul Alam
authors.initials: SA
authors.lastname: Sarker
authors.forename: Shawna
authors.initials: S
authors.lastname: McCallin
authors.forename: Caroline
authors.initials: C
authors.lastname: Barretto
authors.forename: Bernard
authors.initials: B
authors.lastname: Berger
authors.forename: Anne-Cécile
authors.initials: AC
authors.lastname: Pittet
authors.forename: Shamima
authors.initials: S
authors.lastname: Sultana
authors.forename: Lutz
authors.initials: L
authors.lastname: Krause
authors.forename: Sayeda
authors.initials: S
authors.lastname: Huq
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Bibiloni
authors.forename: Anne
authors.initials: A
authors.lastname: Bruttin
authors.forename: Gloria
authors.initials: G
authors.lastname: Reuteler
authors.forename: Harald
authors.initials: H
authors.lastname: Brüssow
mesh_terms: D000284:Administration, Oral; D000328:Adult; D001459:Bangladesh; D001688:Biological Products / Q000008:administration & dosage* / Q000009:adverse effects; D001691:Biological Therapy / Q000379:methods*; D005243:Feces / Q000821:virology; D005260:Female; D006805:Human Experimentation; D006801:Humans; D008297:Male; D010919:Placebos / Q000008:administration & dosage; D013604:T-Phages; D055815:Young Adult
chemical_list: D001688:Biological Products; D010919:Placebos