title: Designed short RGD peptides for one-pot aqueous synthesis of integrin-binding CdTe and CdZnTe quantum dots.
abstract: We have designed a series of short RGD peptide ligands and developed one-pot aqueous synthesis of integrin-binding CdTe and CdZnTe quantum dots (QDs). We first examined the effects of different RGD peptides, including RGDS, CRGDS, Ac-CRGDS, CRGDS-CONH₂, Ac-CRGDS-CONH₂, RGDSC, CCRGDS, and CCCRGDS, on the synthesis of CdTe QDs. CRGDS were found to be the optimal ligand, providing the CdTe QDs with well-defined wavelength ranges (500-650 nm) and relatively high photoluminescence quantum yields (up to 15%). The key synthesis parameters (the pH value of the Cd²⁺-RGD precursors and the molar ratio of RGD/Cd²⁺) were assessed. In order to further improve the optical properties of the RGD-capped QDs, zinc was then incorporated by the simultaneous reaction of Cd²⁺ and Zn²⁺ with NaHTe. By using a mixture of CRGDS and cysteine as the stabilizer, the quantum yields of CdZnTe alloy QDs reached as high as 60% without any post-treatment, and they also showed excellent stability against time, pH, and salinity. Note that these properties could not be obtained with CRGDS or cysteine alone as the stabilizer. Finally, we demonstrated that the RGD-capped QDs preferentially bind to cell surfaces because of the specific recognition of the RGD sequence to cell surface integrin receptors. Our synthesis strategy based on RGD peptides thus represents a convenient route for opening up QD technologies for cell-specific tagging and labeling applicable to a wide range of diagnostics and therapy.
authors.affiliation: State Key Laboratory of Heavy Oil Processing and the Centre for Bioengineering and Biotechnology, China University of Petroleum-East China, 66 Changjiang West Road, Qingdao 266580, China.
authors.forename: Hua
authors.initials: H
authors.lastname: He
authors.forename: Min
authors.initials: M
authors.lastname: Feng
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.forename: Cuixia
authors.initials: C
authors.lastname: Chen
authors.forename: Jiqian
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Wang
authors.forename: Hai
authors.initials: H
authors.lastname: Xu
authors.forename: Jian R
authors.initials: JR
authors.lastname: Lu
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry*; D003460:Crystallization / Q000379:methods; D018922:HL-60 Cells; D006367:HeLa Cells; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D008422:Materials Testing; D008856:Microscopy, Fluorescence / Q000379:methods*; D009842:Oligopeptides / Q000737:chemistry / Q000493:pharmacokinetics*; D045663:Quantum Dots; D013347:Subcellular Fractions / Q000378:metabolism* / Q000648:ultrastructure*; D013691:Tellurium / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry
chemical_list: D019187:Cadmium Compounds; D016023:Integrins; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D015032:Zinc; D013691:Tellurium; C028337:cadmium telluride