title: [Expression levels of Slc7a11 in the skin of Kazakh sheep with different coat colors].
abstract: Slc7a11 belongs to solute transporter gene family, encoding cystine/glutamate transporter xCT. It regulates switching between eumelanin and pheomelanin synthesis. In the present study, Real-time PCR was used to detect the mRNA expression levels of Slc7a11 in the skin of Kazakh lambs with different coat colors (black, brown and white), and then the prokaryotic expression plasmid PET-32a-sxCT was constructed to induce the expression of fusion protein. The target pro-tein was purified by Ni-NTA affinity chromatographic separation, and then was used to immunize rabbit in order to produce rabbit anti-sxCT polyclonal antibody. Finally, the expression levels of sxCT were detected in the skin of Kazakh lambs with different hair colors by Western blotting analysis. Results showed that the mRNA expression levels of Slc7a11 differed significantly in the skin of Kazakh lambs with different coat colors, with the highest level in brown coat color, followed by the black, and then the white. The sxCT protein was also detected in the skin of different coat colors by polyclonal antibody, with the highest level in brown coat color, followed by the black, and then the white. It is, therefore, concluded that slc7a11 gene might be associated with the phenotype of coat color in Kazakh sheep.
authors.affiliation: College of Animal Science and Tecnology, Shihezi University, Shihezi 832003, China. lht_fangfang@yahoo.com.cn
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: He
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Zhou
authors.forename: Song-Hua
authors.initials: SH
authors.lastname: Zhao
authors.forename: Wen-Xiang
authors.initials: WX
authors.lastname: Zhang
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Zong-Sheng
authors.initials: ZS
authors.lastname: Zhao
authors.forename: Bin
authors.initials: B
authors.lastname: Jia
mesh_terms: D027182:Amino Acid Transport System y+ / Q000235:genetics*; D000818:Animals; D015153:Blotting, Western; D003116:Color; D006200:Hair Color; D060888:Real-Time Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D034561:Sheep, Domestic / Q000235:genetics*; D012867:Skin / Q000378:metabolism*
chemical_list: D027182:Amino Acid Transport System y+; D011993:Recombinant Fusion Proteins