title: Three-dimensional Raman spectroscopic imaging of protein crystals deposited on a nanodroplet.
abstract: Confocal Raman spectroscopic imaging has been used to find the location of protein crystals deposited in a nanodroplet. The depth of the protein crystal has been clearly identified by comparing the three-dimensional Raman spectroscopic images of the protein with those of water. Additionally, the low concentration region around a growing protein crystal in the nanodroplet was visualized using two-dimensional Raman spectroscopic imaging.
authors.affiliation: Measurement Solution Research Center, National Institute of Advanced Industrial Science and Technology, 807-1 Shuku, Tosu, Saga 841-0052, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nitahara
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Maeki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Kenichi
authors.initials: K
authors.lastname: Yamashita
authors.forename: Masaya
authors.initials: M
authors.lastname: Miyazaki
authors.forename: Hideaki
authors.initials: H
authors.lastname: Maeda
mesh_terms: D003460:Crystallization; D021621:Imaging, Three-Dimensional / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
chemical_list: D010940:Plant Proteins; C003427:thaumatin protein, plant