title: Molecular characterization of the pathogenic plant  fungus Rhizoctonia solani (Ceratobasidiaceae) isolated from Egypt based  on protein and PCR-RAPD profiles.
abstract: Twenty-one isolates of Rhizoctonia solani were  categorized into three anastomosis groups consisting of AG-4-HG-I (eight  isolates), AG-2-2 (nine isolates) and AG-5 (four isolates). Their pathogenic  capacities were tested on cotton cultivar Giza 86. Pre-emergence damping-off  varied in response to the different isolates; however, the differences were not  significant. Soluble proteins of the fungal isolates were electrophoresed using  SDS-PAGE and gel electrophoreses. A dendrogram of the protein banding patterns  by the UPGMA of arithmetic means placed the fungal isolates into distinct  groups. There was no evidence of a relationship between protein dendrogram, anastomosis  grouping or level of virulence or geographic origin. The dendrogram generated  from these isolates based on PCR analysis with five RAPD-PCR primers showed  high levels of genetic similarity among the isolates from the same geographical  locations. There was partially relationship between the genetic similarity and  AGs or level of virulence or geographic origin based on RAPD dendrogram. These  results demonstrate that RAPD technique is a useful tool in determining the  genetic characterization among isolates of R. solani.
authors.affiliation: Botany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi Arabia. momahmoud@ksu.edu.sa
authors.forename: M A
authors.initials: MA
authors.lastname: Mahmoud
authors.forename: S A
authors.initials: SA
authors.lastname: Al-Sohaibani
authors.forename: A M M
authors.initials: AM
authors.lastname: Abdelbacki
authors.forename: M R
authors.initials: MR
authors.lastname: Al-Othman
authors.forename: A R M
authors.initials: AR
authors.lastname: Abd El-Aziz
authors.forename: K K
authors.initials: KK
authors.lastname: Kasem
authors.forename: M S
authors.initials: MS
authors.lastname: Mikhail
authors.forename: K K
authors.initials: KK
authors.lastname: Sabet
authors.forename: M R
authors.initials: MR
authors.lastname: Omar
authors.forename: E M
authors.initials: EM
authors.lastname: Hussein
mesh_terms: D017931:DNA Primers / Q000378:metabolism; D004534:Egypt; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000235:genetics*; D005843:Geography; D003368:Gossypium / Q000382:microbiology*; D016533:Mycological Typing Techniques; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D012232:Rhizoctonia / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D036226:Seedlings / Q000382:microbiology
chemical_list: D017931:DNA Primers; D005656:Fungal Proteins