title: Effect of silver nanoparticles on human primary keratinocytes.
abstract: Silver nanoparticles (AgNPs) have many biological applications in biomedicine, biotechnology and other life sciences. Depending on the size, shape and the type of carrier, AgNPs demonstrate different physical and chemical properties. AgNPs have strong antimicrobial, antiviral and antifungal activity, thus they are used extensively in a range of medical settings, particularly in wound dressings but also in cosmetics. This study was undertaken to examine the potential toxic effects of 15 nm polyvinylpyrrolidone-coated AgNPs on primary normal human epidermal keratinocytes (NHEK). Cells were treated with different concentrations of AgNPs and then cell viability, metabolic activity and other biological and biochemical aspects of keratinocytes functioning were studied. We observed that AgNPs decrease keratinocyte viability, metabolism and also proliferatory and migratory potential of these cells. Moreover, longer exposure resulted in activation of caspase 3/7 and DNA damage. Our studies show for the first time, that AgNPs may present possible danger for primary keratinocytes, concerning activation of genotoxic and cytotoxic processes depending on the concentration.
authors.affiliation: Department of General Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland.
authors.forename: Radoslaw
authors.initials: R
authors.lastname: Szmyd
authors.forename: Anna Grazyna
authors.initials: AG
authors.lastname: Goralczyk
authors.forename: Lukasz
authors.initials: L
authors.lastname: Skalniak
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Cierniak
authors.forename: Barbara
authors.initials: B
authors.lastname: Lipert
authors.forename: Francesca Larese
authors.initials: FL
authors.lastname: Filon
authors.forename: Matteo
authors.initials: M
authors.lastname: Crosera
authors.forename: Julia
authors.initials: J
authors.lastname: Borowczyk
authors.forename: Eliza
authors.initials: E
authors.lastname: Laczna
authors.forename: Justyna
authors.initials: J
authors.lastname: Drukala
authors.forename: Andrzej
authors.initials: A
authors.lastname: Klein
authors.forename: Jolanta
authors.initials: J
authors.lastname: Jura
mesh_terms: D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D012834:Silver; D053148:Caspase 3; D053179:Caspase 7