title: Characterization of 12 polymorphic microsatellite markers in the liverwort Marchantia inflexa (Marchantiaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed in Marchantia inflexa, a haploid liverwort with unisexual individuals, to identify clonal genotypes and measure population genetic variability.
  
  
  RESULTS
  Twelve polymorphic primer sets were developed from three enriched genomic libraries. Primers were fluorescently labeled, and alleles were identified by fragment analysis. These primers were tested in four natural populations and revealed a moderate level of genetic variation within four populations, as indicated by the number of alleles per locus (range = 1-5).
  
  
  CONCLUSIONS
  Development of polymorphic markers is crucial to the identification of individuals and will allow additional research into this species, particularly on its population genetics and metapopulation dynamics.
authors.affiliation: Department of Biology, University of Kentucky, 101 T. H. Morgan Building, Lexington, Kentucky 40506-0225 USA. jessica.brzyski@uky.edu
authors.forename: Jessica R
authors.initials: JR
authors.lastname: Brzyski
authors.forename: Kelly J
authors.initials: KJ
authors.lastname: Adams
authors.forename: Charlotte M
authors.initials: CM
authors.lastname: Walter
authors.forename: Kristin H
authors.initials: KH
authors.lastname: Gale
authors.forename: D Nicholas
authors.initials: DN
authors.lastname: McLetchie
mesh_terms: D000483:Alleles; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D015698:Genomic Library; D005838:Genotype; D047008:Marchantia / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018744:DNA, Plant