title: Identification of autoantibodies expressed in acquired aplastic anaemia.
abstract: Acquired aplastic anaemia (aAA) is recognized as an autoimmune disorder; however, the autoantigens and target cells involved remain elusive. Expression of autoantibodies and their target cells were examined using the haematopoietic cell line K562 and bone marrow stromal cell line hTS-5; 43·5% and 21·7% of aAA expressed autoantibody against K562 and hTS-5 cells, respectively. The autoantigens were identified by serological identification of antigens through recombinant cDNA expression cloning. This study indicates that haematopoietic cells are the targets of immune abnormality in aAA. These autoantibodies may be utilized to distinguish patients associated with immune abnormality from bone marrow failure syndrome.
authors.affiliation: Division of Laboratory Diagnosis, Sapporo Medical University Hospital, Sapporo, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Goto
authors.forename: Kageaki
authors.initials: K
authors.lastname: Kuribayashi
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondoh
authors.forename: Maki
authors.initials: M
authors.lastname: Tanaka
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kobayashi
authors.forename: Naoki
authors.initials: N
authors.lastname: Watanabe
mesh_terms: D000741:Anemia, Aplastic / Q000235:genetics* / Q000276:immunology* / Q000628:therapy; D001323:Autoantibodies / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D001324:Autoantigens / Q000097:blood / Q000235:genetics / Q000276:immunology; D001327:Autoimmune Diseases / Q000235:genetics / Q000276:immunology / Q000628:therapy; D018118:Chloride Channels / Q000276:immunology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000235:genetics / Q000276:immunology; D007167:Immunotherapy; D020014:K562 Cells; D008667:Metalloproteins / Q000276:immunology; D009687:Nuclear Proteins / Q000276:immunology; D016601:RNA-Binding Proteins / Q000276:immunology; D012269:Ribosomal Proteins / Q000276:immunology
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; C501966:CLIC1 protein, human; D018118:Chloride Channels; D007074:Immunoglobulin G; D008667:Metalloproteins; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; C083686:RPS27 protein, human; D012269:Ribosomal Proteins