title: Protective effect of vitamins e and C on endosulfan-induced reproductive toxicity in male rats.
abstract: BACKGROUND
  The role of oxidative stress in endosulfan-induced reproductive toxicity has been implicated. This study was performed to evaluate the possible protective effect of vitamins E and C, against endosulfan-induced reproductive toxicity in rats.
  
  
  METHODS
  Fifty adult male Sprague-Dawley rats were randomly divided into five groups (n=10 each). The groups included a control receiving vehicle, a group treated with endosulfan (10 mg/kg/day) alone, and three endosulfan-treated group receiving vitamin C (20 mg/kg/day), vitamin E (200 mg/kg/day), or vitamine C+vitamin E at the same doses. After 10 days of treatment, sperm parameters, plasma lactate dehydrogenase (LDH), plasma testosterone and malondialdehyde (MDA) levels in the testis were determined.
  
  
  RESULTS
  Oral administration of endosulfan caused a reduction in the sperm motility, viability, daily sperm production (DSP) and increased the number of sperm with abnormal chromatin condensation. Endosulfan administration increased testis MDA and plasma LDH. Supplementation of vitamin C and vitamin E to endosulfan-treated rats reduced the toxic effect of endosulfan on sperm parameters and lipid peroxidation in the testis. Vitamin E was more protective than vitamin C in reducing the adverse effects of the endosulfan.
  
  
  CONCLUSIONS
  The findings data suggest that administration of vitamins C and E ameliorated the endosulfan-induced oxidative stress and sperm toxicity in rat. The effect of vitamin E in preventing endosulfan-induced sperm toxicity was superior to that of vitamin C.
authors.affiliation: Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran. ; Department of Laboratory Sciences, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Takhshid
authors.forename: Ali Reza
authors.initials: AR
authors.lastname: Tavasuli
authors.forename: Yazdan
authors.initials: Y
authors.lastname: Heidary
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Keshavarz
authors.forename: Hussain
authors.initials: H
authors.lastname: Kargar
keywords: Endosulfan; oxidative stress; spermatogenesis; vitamin C; vitamin E