title: The impact of intratracheally instilled carbon black on the cardiovascular system of rats: elevation of blood homocysteine and hyperactivity of platelets.
abstract: Carbon black (CB) is an industrial chemical with high potential for human exposure. Although the relationship between exposure to particulate matter (PM) and cardiovascular disease is well documented, the risk of adverse cardiovascular effects attributed to CB particles has not been clearly characterized. This study was performed to (1) investigate the effects of CB on cardiovascular system and (2) identify the target tissue or potential biomarkers. Carbon black with a distinct particle size, N330 (ultrafine particle) and N990 (fine particle), was intratracheally instilled into rats at a doses of 1, 3, or 10 mg/kg. Measurements of thrombotic activity and determination of plasma homocysteine levels, cardiac functionality, and inflammatory responses were conducted at 24-h and 1-wk time points. Exposure to N330 accelerated platelet-dependent blood clotting at 10 mg/kg, the highest exposure tested. Unexpectedly, both N330 and N990 led to prolongation of activated partial thromboplastin time (aPTT), whereas these CB particles failed to affect prothrombin time (PT). N990 produced a significant elevation in the level of plasma homocysteine, a well-established etiological factor in cardiovascular diseases. Both N330 and N990 induced apparent inflammation in the lungs; however, both particles failed to initiate systemic inflammation. Neither CB particle produced observable cardiac symptoms as detected by electrocardiography. Taken together, data show CB exposure enhanced the cardiovascular risk by inducing hyperhomocysteinemia and platelet hyperactivity, although these effects may be variable depending on particle size and exposure duration. Homocysteine may be a potential biomarker for cardiovascular toxicity following CB exposure.
authors.affiliation: College of Pharmacy, Dongguk University, Goyang, Gyeonggi-do, Republic of Korea.
authors.forename: Hwa
authors.initials: H
authors.lastname: Kim
authors.forename: Seok-Jeong
authors.initials: SJ
authors.lastname: Oh
authors.forename: Hui-Chan
authors.initials: HC
authors.lastname: Kwak
authors.forename: Jong-Kyu
authors.initials: JK
authors.lastname: Kim
authors.forename: Cheol-Hong
authors.initials: CH
authors.lastname: Lim
authors.forename: Jeong-Sun
authors.initials: JS
authors.lastname: Yang
authors.forename: Kwangsik
authors.initials: K
authors.lastname: Park
authors.forename: Sang-Kyum
authors.initials: SK
authors.lastname: Kim
authors.forename: Moo-Yeol
authors.initials: MY
authors.lastname: Lee
mesh_terms: D000395:Air Pollutants, Occupational / Q000737:chemistry / Q000633:toxicity; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001777:Blood Coagulation / Q000187:drug effects; D002274:Carcinogens, Environmental / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D002318:Cardiovascular Diseases / Q000209:etiology*; D002319:Cardiovascular System / Q000187:drug effects / Q000276:immunology / Q000503:physiopathology; D004305:Dose-Response Relationship, Drug; D006710:Homocysteine / Q000097:blood; D020138:Hyperhomocysteinemia / Q000097:blood / Q000139:chemically induced* / Q000503:physiopathology; D007322:Instillation, Drug; D008168:Lung / Q000187:drug effects / Q000276:immunology; D008297:Male; D009928:Organ Specificity; D010316:Particle Size; D010974:Platelet Aggregation / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D053260:Soot / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*
chemical_list: D000395:Air Pollutants, Occupational; D015415:Biomarkers; D002274:Carcinogens, Environmental; D053260:Soot; D006710:Homocysteine