title: Highly specific revelation of rat serum glycopeptidome by boronic acid-functionalized mesoporous silica.
abstract: Although the specific profiling of endogenous glycopeptides in serum is highly inclined towards the discovery of disease biomarkers, studies on the endogenous glycopeptides (glycopeptidome) have never been conducted because of several factors. These factors include the high dynamic range of serum proteins, the inadequacy of traditional sample preparation techniques in proteomics for low-molecular-weight (LMW) proteins, and the relatively low abundances of glycopeptides. Boronic acid-functionalized mesoporous silica was synthesized in this study to overcome the limitations of the state-of-the-art methods for glycopeptidome research. The boronic acid-functionalized mesoporous silica exhibited excellent selectivity by analyzing glycopeptides in the mixture of glycopeptides/non-glycopeptides at molar ratio of 1:100, extreme sensitivity (the limit of detection was at the fmol level), good binding capacity (40 mg g(-1)), as well as the high post-enrichment recovery of glycopeptides (up to 88.10%). The as-prepared material possessing both glycopeptide-suitable pore size and glycopeptide-specific selectivity has shown special capability for enriching the endogenous glycopeptides. Fifteen unique glycosylation sites mapped to 15 different endogenous glycopeptides were identified in rat serum. The established protocol revealed for the first time the rat serum glycopeptidome.
authors.affiliation: Shanghai Cancer Center and Department of Chemistry, Fudan University, Shanghai 200032, PR China.
authors.forename: Liting
authors.initials: L
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Guoquan
authors.initials: G
authors.lastname: Yan
authors.forename: Jun
authors.initials: J
authors.lastname: Yao
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Yang
authors.forename: Haojie
authors.initials: H
authors.lastname: Lu
mesh_terms: D000327:Adsorption; D000818:Animals; D001897:Boronic Acids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D006020:Glycopeptides / Q000097:blood*; D006031:Glycosylation; D016062:Porosity; D040901:Proteomics; D051381:Rats; D012822:Silicon Dioxide / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001897:Boronic Acids; D006020:Glycopeptides; C509968:MCM-41; D012822:Silicon Dioxide