title: The population structure of Salmonella enterica Enteritidis in Iran analyzed by multiple-locus variable-number tandem repeat analysis.
abstract: Salmonella enterica Enteritidis is the most frequent etiological agent of salmonellosis in humans and poultry. To understand the genetic diversity of S. Enteritidis in Iran, we examined 69 chicken isolates from 18 broiler farms and six non-epidemic human isolates from six geographically distant provinces by multi-locus variable-number tandem repeat analysis (MLVA). Among SE2, SE3, SE5, SE7, SE8, SENTR4, and SENTR7, only SE5 with four and SENTR7 with two alleles, respectively, proved variable giving estimates of locus genetic diversity of 0.58 and 0. In all, six closely related MLVA profiles were identified among which three were commonly represented by human and chicken isolates. This population homogeneity contrasts with the high diversity at these loci reported elsewhere and is likely a consequence of a single clone of S. Enteritidis distributed across Iran.
authors.affiliation: Veterinary Aerobic Bacterial Research and Vaccine Production Department, Razi Vaccine and Serum Research Institute, Karaj, 3197619751, Iran.
authors.forename: Rainak
authors.initials: R
authors.lastname: Ghaderi
authors.forename: Keyvan
authors.initials: K
authors.lastname: Tadayon
authors.forename: Sargis
authors.initials: S
authors.lastname: Avagyan
authors.forename: Pejvak
authors.initials: P
authors.lastname: Khaki
authors.forename: Soheila Moradi
authors.initials: SM
authors.lastname: Bidhendi
authors.forename: Ken James
authors.initials: KJ
authors.lastname: Forbes
authors.forename: Nader
authors.initials: N
authors.lastname: Mosavari
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Toroghi
authors.forename: Farhad
authors.initials: F
authors.lastname: Moosakhani
authors.forename: Reza
authors.initials: R
authors.lastname: Banihashemi
authors.forename: Mohamad
authors.initials: M
authors.lastname: Sekhavati
authors.forename: Nasim
authors.initials: N
authors.lastname: Karimnasab
mesh_terms: D000818:Animals; D002645:Chickens; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007492:Iran; D018598:Minisatellite Repeats; D058885:Multilocus Sequence Typing / Q000379:methods; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011201:Poultry Diseases / Q000235:genetics / Q000382:microbiology*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000145:classification* / Q000235:genetics; D018709:Statistics, Nonparametric
chemical_list: D004269:DNA, Bacterial