title: Possible role of a septin, SEPT1, in spreading in squamous cell carcinoma DJM-1 cells.
abstract: We performed biochemical, histochemical and cell biological characterization of septins by focusing on SEPT1 in human skin tissues and a squamous cell carcinoma (SCC) cell line DJM-1. In immunoblotting, SEPT1, together with other septins, was detected in normal human epidermis, SCC and DJM-1. In immunohistochemical analyses, SEPT1 was detected diffusely in the cytoplasm of human epidermal cells and eccrine gland epithelial cells, and the protein level was increased in some skin tumors. In DJM-1 cells, SEPT1 together with other members of SEPT2-subgroup, SEPT4 and SEPT5, was enriched in lamellipodia and the localization was dependent on the cortical actin structure. SEPT1 distribution at lamellipodia was also observed in melanoma B16 cells. SEPT9, SEPT11 and SEPT14, in contrast, were localized along with microtubules in DJM-1 cells. In immunoprecipitation assays, SEPT1 and SEPT5 were found to form a complex in DJM-1 cells, whereas SEPT9, SEPT11 and SEPT14 formed a distinct complex with other septins including SEPT7, SEPT8 and SEPT10, in which SEPT5 was not included. When SEPT1 was silenced in DJM-1 cells, cell spreading was inhibited. These results suggest that SEPT1 may participate in cell-cell and/or cell-substrate interaction in DJM-1 and exert its function in a coordinated manner with other septins.
authors.affiliation: Department of Dermatology, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Mizutani
authors.forename: Hidenori
authors.initials: H
authors.lastname: Ito
authors.forename: Ikuko
authors.initials: I
authors.lastname: Iwamoto
authors.forename: Rika
authors.initials: R
authors.lastname: Morishita
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kanoh
authors.forename: Mariko
authors.initials: M
authors.lastname: Seishima
authors.forename: Koh-ichi
authors.initials: K
authors.lastname: Nagata
mesh_terms: D000818:Animals; D019556:COS Cells; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D051379:Mice; D058112:Septins / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: C553082:SEPTIN1 protein, human; D058112:Septins