title: Occurrence and structure of cyclic Enterobacterial Common Antigen in Escherichia coli O157:H⁻.
abstract: Two cyclic forms of the Enterobacterial Common Antigen were isolated from Escherichia coli O157:H(-). These antigenic determinants were purified from the biomass through extensive chemical, enzymatic and chromatographic procedures whereas MALDI MS spectrometry indicated their cyclic nature with a polymerization degree of 4 or 5. The two species, denoted as ECA(CYC-4) and ECA(CYC-5), were assigned by NMR and showed no further substitution with other appendages such as acetyl groups as usually described for similar cyclic antigens from other Enterobacteriaceae.
authors.affiliation: Università di Napoli Federico II, Dipartimento di Scienze Chimiche, Complesso Universitario Monte Sant'Angelo, Via Cintia 4, 80126 Napoli, Italy.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Fregolino
authors.forename: Radka
authors.initials: R
authors.lastname: Ivanova
authors.forename: Rosa
authors.initials: R
authors.lastname: Lanzetta
authors.forename: Antonio
authors.initials: A
authors.lastname: Molinaro
authors.forename: Michelangelo
authors.initials: M
authors.lastname: Parrilli
authors.forename: Tsvetelina
authors.initials: T
authors.lastname: Paunova-Krasteva
authors.forename: Stoyanka R
authors.initials: SR
authors.lastname: Stoitsova
authors.forename: Cristina
authors.initials: C
authors.lastname: De Castro
mesh_terms: D000942:Antigens, Bacterial / Q000737:chemistry* / Q000378:metabolism*; D019453:Escherichia coli O157 / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry
chemical_list: D000942:Antigens, Bacterial; D011108:Polymers; C017286:enterobacterial common antigen