title: Progress towards Drosophila epithelial cell culture.
abstract: Drosophila epithelial research is at the forefront of the field; however, there are no well-characterized epithelial cell lines that could provide a complementary in vitro model for studies conducted in vivo. Here, a protocol is described that produces epithelial cell lines. The method uses genetic manipulation of oncogenes or tumor suppressors to induce embryonic primary culture cells to rapidly progress to permanent cell lines. It is, however, a general method and the type of cells that comprise a given line is not controlled experimentally. Indeed, only a small fraction of the lines produced are epithelial in character. For this reason, additional work needs to be done to develop a more robust epithelial cell-specific protocol. It is expected that Drosophila epithelial cell lines will have great utility for in vitro analysis of epithelial biology, particularly high-throughput analyses such as RNAi screens.
authors.affiliation: Department of Molecular Genetics, Ohio State University, Columbus, OH, USA. Simcox.1@osu.edu
authors.forename: Amanda
authors.initials: A
authors.lastname: Simcox
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D015925:Cryopreservation; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster / Q000166:cytology*; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D016147:Genes, Tumor Suppressor; D008297:Male; D009857:Oncogenes / Q000235:genetics; D010063:Ovum / Q000166:cytology; D034622:RNA Interference; D014157:Transcription Factors / Q000235:genetics; D014162:Transfection; D018631:ras Proteins / Q000235:genetics
chemical_list: D015820:Cadherins; D029721:Drosophila Proteins; C557617:GAL4 protein, Drosophila; D014157:Transcription Factors; D018631:ras Proteins