title: Detection of human rotavirus in hospitalized diarrheic children in central India.
abstract: During the present study, group A human rotaviruses were detected among diarrheic children using polyacrylamide gel electrophoresis (PAGE) technique, with a typical RNA migration pattern of 4:2:3:2, suggestive of group A rotavirus. During the study, a total of 46 fecal samples collected from hospitalized children with acute diarrhea as well as children inhabiting nearby animal farms with history of presence of animal rotaviruses on the farms were processed for detection of human rotavirus. Out of 33 diarrheic children, 12 showed presence of rotavirus infection (36.36%), however, none of the children from animal farm areas showed presence of rotavirus. Female children were more susceptible to rotavirus infection (46.15%) than males (30%). Majority of the cases of rotavirus gastroenteritis belonged up to one year of the age, with an incidence of 40.91%. RNA profile of rotaviruses suggested circulation of 5 different electropherotypes in this geographical locale of the country, indicating existence of genomic diversity among human rotaviruses. Majority of the isolates were of long pattern (66.67%), whereas short pattern was detected only in one third of the viruses. This preliminary study emphasizes for further detailed studies on the molecular characterization of rotaviruses circulating in this part of country and their relationship with other human rotavirus strains and animal strains in the country.
authors.affiliation: College of Veterinary Science & A.H., Jawaharlal Nehru Krishi, Vishwa Vidyalaya, Jabalpur, 482 001 India.
authors.forename: Anupam Lal
authors.initials: AL
authors.lastname: Kusumakar
authors.lastname: Savita
authors.forename: Yashpal
authors.initials: Y
authors.lastname: Malik
authors.lastname: Minakshi
authors.forename: Gaya
authors.initials: G
authors.lastname: Prasad
keywords: Diarrhea; Group A human rotavirus; RNA-PAGE; detection; electropherotypes