title: Dominant-negative and knockdown approaches to studying PPAR activity.
abstract: Manipulation of PPAR activity is often a valuable approach toward elucidation of the cellular effects of PPARs. The activity of specific PPARs can be decreased using chemical inhibitors, but these approaches can be affected by nonspecific interactions or cell toxicity. Alternative approaches include targeting PPAR gene expression or activity through molecular biology strategies. Here, we describe the targeting of PPARγ through dominant-negative and siRNA-mediated knockdown constructs.
authors.affiliation: VA Nebraska and Western Iowa Health Care System, Omaha, NE, USA.
authors.forename: Sudhir
authors.initials: S
authors.lastname: Singh
authors.forename: Robert G
authors.initials: RG
authors.lastname: Bennett
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000502:physiology; D015153:Blotting, Western; D055785:Gene Knockdown Techniques / Q000379:methods*; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics; D009154:Mutation; D047492:Peroxisome Proliferator-Activated Receptors / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020218:Response Elements / Q000235:genetics; D019562:Viral Load
chemical_list: D047492:Peroxisome Proliferator-Activated Receptors; D034741:RNA, Small Interfering; D008156:Luciferases