title: Validation of antibiotics in catfish by on-line solid phase extraction coupled to liquid chromatography tandem mass spectrometry.
abstract: For the first time automated on-line solid phase extraction coupled to liquid chromatography tandem mass spectrometry was developed for the simultaneous determination of 13 antibiotics (sulfonamides and tetracyclines) in catfish. The method proposed was validated according to Commission Decision 2002/657/EC, showing good linearity between 2 and 350 μg kg(-1), high recovery (80-99%) and reproducibility (13-20%) values, lower detection limits than 0.1 μg kg(-1), and quantification limits under 2.4 μg kg(-1) (between 39 and 84 times lower than the MRL fixed by the EU). Moreover, the proposed method was also used to determine sulfonamides and tetracyclines in 16 out of 107 samples, all previously analysed by microbiological screening that gave positive results. Five out of 13 antibiotics were found, having tetracycline the higher occurrence (10 samples); in all cases the concentrations were lower than the MRL established.
authors.affiliation: Sailab, Argenters, 5 Ed. I. Bajos D, Parc tecnológic del Vallès, 08290 Cerdanyola del Vallès, Spain.
authors.forename: Jorge
authors.initials: J
authors.lastname: Hurtado de Mendoza
authors.forename: Luana
authors.initials: L
authors.lastname: Maggi
authors.forename: Liliana
authors.initials: L
authors.lastname: Bonetto
authors.forename: Beatriz
authors.initials: B
authors.lastname: Rodríguez Carmena
authors.forename: Alicia
authors.initials: A
authors.lastname: Lezana
authors.forename: Francisco A
authors.initials: FA
authors.lastname: Mocholí
authors.forename: Manuel
authors.initials: M
authors.lastname: Carmona
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000302:isolation & purification*; D002397:Catfishes; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis / Q000302:isolation & purification; D005506:Food Contamination / Q000032:analysis*; D017747:Seafood / Q000032:analysis*; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents