title: Chondracanthus tenellus (Harvey) hommersand extract protects the human keratinocyte cell line by blocking free radicals and UVB radiation-induced cell damage.
abstract: The aim of this study was to investigate the protective effects of the ethanol extract of the red algae Chondracanthus tenellus (Harvey) Hommersand (CTE) on cultured human keratinocyte cell line. The cellular protection conferred by CTE was evidenced by the ability of the extract to absorb ultraviolet B (UVB; 280-320 nm) and to scavenge the radical 1,1-diphenyl-2-picrylhydrazyl, as well as intracellular reactive oxygen species (ROS), induced by either hydrogen peroxide (H(2)O(2)) or UVB radiation. In addition, both superoxide anion generated by the xanthine/xanthine oxidase system and hydroxyl radical generated by the Fenton reaction (FeSO(4) + H(2)O(2)) were scavenged by CTE, as confirmed using electron spin resonance spectrometry. In the human keratinocyte cell line, CTE decreased the degree of injury resulting from UVB-induced oxidative stress to lipids, proteins, and DNA. CTE-treated cells also showed a reduction in UVB-induced apoptosis, as exemplified by fewer apoptotic bodies and less DNA fragmentation. Taken together, these results suggest that CTE confers protection on the human keratinocyte cell line against UVB-induced oxidative stress by absorbing UVB ray and scavenging ROS, thereby reducing injury to cellular constituents.
authors.affiliation: School of Medicine, Jeju National University, Jeju, 690-756, Republic of Korea.
authors.forename: Mei Jing
authors.initials: MJ
authors.lastname: Piao
authors.forename: Yu Jae
authors.initials: YJ
authors.lastname: Hyun
authors.forename: Tae-Heon
authors.initials: TH
authors.lastname: Oh
authors.forename: Hee Kyoung
authors.initials: HK
authors.lastname: Kang
authors.forename: Eun Sook
authors.initials: ES
authors.lastname: Yoo
authors.forename: Young Sang
authors.initials: YS
authors.lastname: Koh
authors.forename: Nam Ho
authors.initials: NH
authors.lastname: Lee
authors.forename: In Soo
authors.initials: IS
authors.lastname: Suh
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun
mesh_terms: D002460:Cell Line; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D000461:Rhodophyta / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D005609:Free Radicals; D010936:Plant Extracts