title: Protective role of standardized Feronia limonia stem bark methanolic extract against carbon tetrachloride induced hepatotoxicity.
abstract: OBJECTIVE
  This study evaluates hepatoprotective potential of Feronia limonia stem bark (FSB) extracts and fractions using experimental models.
  
  
  METHODS
  Activity levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and cell viability were evaluated in HepG2 cells treated with carbon tetrachloride (CCl4) in presence or absence of FL extracts or fractions. Also, plasma markers of hepatic damage, hepatic antioxidants, lipid peroxidation and histopathological alterations were assessed in rats treated with CCl4 alone or in combination with 200 or 400 mg/kg bodyweight (BW) of FSB-7 or 25 mg/kg BW of silymarin.
  
  
  RESULTS
  In vitro co-supplementation of FSB extracts or fractions recorded varying degree of hepatoprotective potentials. Also, pre-supplementation of FSB methanolic extract (FSB-7) followed by CCl4 treatment significantly prevented hepatic damage and depletion of cellular antioxidants. Also, CCl4+ FSB-7 group showed minimal distortion in the histoarchitecture of liver and results were comparable to that of CCl4+ silymarin treated rats.
  
  
  CONCLUSIONS
  This inventory is the first scientific report on hepatoprotective potential of FSB methanolic extract.
authors.affiliation: Herbal Drug Technology Laboratory, Pharmacognosy Laboratory, Faculty of Pharmaceutical Sciences, Shoolini University, Himachal Pradesh, India. mjainms@yahoo.com
authors.forename: Mahendra
authors.initials: M
authors.lastname: Jain
authors.forename: Rakhee
authors.initials: R
authors.lastname: Kapadia
authors.forename: Ravirajsinh Navalsinh
authors.initials: RN
authors.lastname: Jadeja
authors.forename: Menaka Chanu
authors.initials: MC
authors.lastname: Thounaojam
authors.forename: Ranjitsinh Vijaysinh
authors.initials: RV
authors.lastname: Devkar
authors.forename: Shri Hari
authors.initials: SH
authors.lastname: Mishra
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002251:Carbon Tetrachloride; D002470:Cell Survival / Q000187:drug effects; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D019610:Cytoprotection; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D000432:Methanol / Q000737:chemistry; D018384:Oxidative Stress / Q000187:drug effects; D024301:Plant Bark; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D051381:Rats; D017208:Rats, Wistar; D027821:Rutaceae / Q000737:chemistry; D012838:Silymarin / Q000494:pharmacology; D012997:Solvents / Q000737:chemistry
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D010936:Plant Extracts; D012838:Silymarin; D012997:Solvents; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D001663:Bilirubin; D000432:Methanol