title: Identification of an MHC class I ligand for the single member of a killer cell lectin-like receptor family, KLRH1.
abstract: Natural killer cells are able to recognize and kill target cells according to differences in MHC class I expression. In rodents, the Ly49 receptors are primarily responsible for this MHC differentiation. We previously described the cloning of a novel C-type lectin-like receptor, KLRH1, encoded in the NK complex adjacent to the Ly49 genes and expressed by subsets of NK and NKT cells. MHC influence on selection of KLRH1(+) NK cells in congenic strains suggested that KLRH1 may have an MHC ligand, although we were unable to identify any such ligand. In this study, we have used a sensitive reporter system and Fc fusion protein to demonstrate that KLRH1 binds specifically to the classical MHC class I molecule RT1-A2 of the RT1(n) haplotype. Cytolytic activity of KLRH1-transfected RNK-16 cells was also inhibited by target cells expressing RT1-A2(n). Thus, KLRH1 represents a novel family of MHC allele-specific inhibitory receptors expressed by NK cells.
authors.affiliation: Department of Anatomy, University of Oslo, 0317 Oslo, Norway. m.r.daws@medisin.uio.no
authors.forename: Michael R
authors.initials: MR
authors.lastname: Daws
authors.forename: Ke-Zheng
authors.initials: KZ
authors.lastname: Dai
authors.forename: Severin
authors.initials: S
authors.lastname: Zinöcker
authors.forename: Christian
authors.initials: C
authors.lastname: Naper
authors.forename: Lise
authors.initials: L
authors.lastname: Kveberg
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Hedrich
authors.forename: Bent
authors.initials: B
authors.lastname: Rolstad
authors.forename: John T
authors.initials: JT
authors.lastname: Vaage
mesh_terms: D000483:Alleles; D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D015870:Gene Expression; D017930:Genes, Reporter; D006239:Haplotypes; D006649:Histocompatibility Antigens / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D008024:Ligands; D055650:NK Cell Lectin-Like Receptor Subfamily A / Q000235:genetics / Q000276:immunology; D011485:Protein Binding; D020033:Protein Isoforms / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D051381:Rats; D011971:Receptors, Immunologic / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014162:Transfection
chemical_list: D006649:Histocompatibility Antigens; C456699:Klrh1 protein, rat; D008024:Ligands; D055650:NK Cell Lectin-Like Receptor Subfamily A; D020033:Protein Isoforms; D011971:Receptors, Immunologic; D011993:Recombinant Fusion Proteins; C038353:histocompatibility antigens RT, rat