title: Self-assembly of conjugated polymer on hybrid nanospheres for cellular imaging applications.
abstract: A new kind of hybrid core-shell nanosphere was fabricated by combining the in situ formation of Au nanoparticles and covalent cross-linking of biocompatible carboxymethyl starch dialdehyde (CMSD) and chitosan (CTS). When the fluorescent dye poly[9,9'-bis(6″-(N,N,N-trimethylammonium)-hexyl)fluorene-2,7-ylenevinylene-co-alt-1,4-phenylene dibromide] (PFV) was assembled on the surface of the hybrid nanospheres through electrostatic attraction, these biocompatible hybrid nanospheres exhibited metal-enhanced fluorescence effects. The fluorescence intensity of (CTS-Au)@CMSD/PFV hybrid nanosphere is 1.43 times that of CTS-CMSD/PFV hybrid nanospheres lacking Au nanoparticle. In addition, the (CTS-Au)@CMSD/PFV hybrid nanospheres exhibit excellent biodegradability upon exposure to enzymatic aqueous solution and good biocompatibility when cocultured with HeLa cervical carcinoma cells; these advantages make them attractive for cellular imaging and biological analysis and detection.
authors.affiliation: State Key Laboratory for Advanced Metals and Materials, School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, P. R. China.
authors.forename: Bihua
authors.initials: B
authors.lastname: Xia
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: He
authors.forename: Qianling
authors.initials: Q
authors.lastname: Cui
authors.forename: Lidong
authors.initials: L
authors.lastname: Li
mesh_terms: D003287:Contrast Media / Q000138:chemical synthesis*; D006367:HeLa Cells; D006801:Humans; D008422:Materials Testing; D008856:Microscopy, Fluorescence / Q000379:methods*; D054118:Nanospheres / Q000737:chemistry*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*
chemical_list: D003287:Contrast Media; D011108:Polymers