title: Label-acquired magnetorotation for biosensing: An asynchronous rotation assay.
abstract: This paper presents a novel application of magnetic particles for biosensing, called label-acquired magnetorotation (LAM). This method is based on a combination of the traditional sandwich assay format with the asynchronous magnetic bead rotation (AMBR) method. In label-acquired magnetorotation, an analyte facilitates the binding of a magnetic label bead to a nonmagnetic solid phase sphere, forming a sandwich complex. The sandwich complex is then placed in a rotating magnetic field, where the rotational frequency of the sandwich complex is a function of the amount of analyte attached to the surface of the sphere. Here, we use streptavidin-coated beads and biotin-coated particles as analyte mimics, to be replaced by proteins and other biological targets in future work. We show this sensing method to have a dynamic range of two orders of magnitude.
authors.affiliation: The University of Michigan, Department of Biomedical Engineering, 2200 Bonisteel, Ann Arbor, MI 48109-2099, United States ; The University of Michigan, Department of Chemistry, 930 North University, Ann Arbor, MI 48109-1055, United States.
authors.forename: Ariel
authors.initials: A
authors.lastname: Hecht
authors.forename: Paivo
authors.initials: P
authors.lastname: Kinnunen
authors.forename: Brandon
authors.initials: B
authors.lastname: McNaughton
authors.forename: Raoul
authors.initials: R
authors.lastname: Kopelman