title: Potent suppression of agglutinability of human A and B type erythrocytes by KMnO(4)-EDTA concerted action.
abstract: OBJECTIVE
  Study was focused to effectively suppress the agglutinability of human A and B type erythrocyte by concerted chemical oxidation approach. Consequently some major bio-physiological consequences of human erythrocytes were analyzed.
  
  
  METHODS
  Concerted chemical oxidation of human A and B type erythrocyte were done by potassium permanganate (KMnO(4)) in presence of ethylene diamine tetra-acetic acid (EDTA). All the chemical reactions were carried out at 37°C temperature and pH 7.4 in phosphate buffer. Finally, suppression of agglutinability were determined by using anti-A and anti-B antisera.
  
  
  RESULTS
  A marked suppression in agglutinability was observed after chemical treatment on human A and B type erythrocytes in vitro. Effective suppression of agglutinability was observed in 0.57 mM concentration of KMnO(4) in presence of 0.9 mM EDTA at optimum condition.
  
  
  CONCLUSIONS
  Results showed that KMnO(4)-EDTA concerted action is feasible. The future work is directed towards the universal human erythrocytes preparation from A and B type human erythrocytes.
authors.affiliation: Department of Chemistry, Indian Institute of Technology, Kharagpur, 721 302 India.
authors.forename: Dipankar
authors.initials: D
authors.lastname: Ghosh
authors.forename: Susmita
authors.initials: S
authors.lastname: Santra
authors.forename: Sujit
authors.initials: S
authors.lastname: Dutta
authors.forename: Panchanan
authors.initials: P
authors.lastname: Pramanik
keywords: Agglutination; Antibodies; Concerted action; Erythrocyte; Transfusion medicine