title: Burkholderia grimmiae sp. nov., isolated from a xerophilous moss (Grimmia montana).
abstract: A Gram-staining-negative, rod-shaped, non-spore-forming bacterium, designated strain R27(T), was isolated from the moss Grimmia montana, collected from Beijing Songshan National Nature Reserve, China, and characterized by using a polyphasic taxonomic approach. The predominant fatty acids of strain R27(T) were C18:1ω7c (33.6%), C16:0 (16.3%), summed feature 3 (C16:1ω7c and/or C16:1ω6c; 15.8%) and C17:0 cyclo (8.7%) and its major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, three uncharacterized aminolipids and an unknown phospholipid. Strain R27(T) contained Q-8 as the dominant isoprenoid quinone and the G+C content of its genomic DNA was 64.6 mol%. On the basis of 16S rRNA gene sequence comparison, strain R27(T) showed 99.1% similarity to the closest related type strain, Burkholderia zhejiangensis OP-1(T), and 97.6% similarity to Burkholderia glathei ATCC 29195(T). However, the DNA-DNA relatedness between strain R27(T) and B. zhejiangensis CCTCC AB 2010354(T) and B. glathei ATCC 29195(T) was 10.2 and 14.9%, respectively. Based on 16S rRNA and rpoB gene sequence similarities and phenotypic and chemotaxonomic data, strain R27(T) is considered to represent a novel species of the genus Burkholderia, for which the name Burkholderia grimmiae sp. nov. is proposed. The type strain is R27(T) (=CGMCC 1.11013(T) =DSM 25160(T)).
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Tian
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Bi He
authors.initials: BH
authors.lastname: Kong
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Su Lin
authors.initials: SL
authors.lastname: Liu
authors.affiliation: New Technique Centre, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Chun Li
authors.initials: CL
authors.lastname: Li
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Yu
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, PR China.
authors.forename: Yan Hong
authors.initials: YH
authors.lastname: Li
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D044002:Bryophyta / Q000382:microbiology*; D019117:Burkholderia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8