title: Cell uptake survey of pegylated nanographene oxide.
abstract: Graphene and more specifically, nanographene oxide (GO) has been proposed as a highly efficient antitumoral therapy agent. Nevertheless, its cell uptake kinetics, its influence in different types of cells and the possibility of controlling cellular internalization timing, is still a field that remains unexplored. Herein, different cell types have been cultured in vitro for several incubation periods in the presence of 0.075 mg ml(-1) pegylated GO solutions. GO uptake kinetics revealed differences in the agent's uptake amount and speed as a function of the type of cell involved. Osteoblast-like cells GO uptake is higher and faster without resulting in greater cell membrane damage. Moreover, the dependence on the commonly used PEG nature (number of branches) also influences the viability and cell uptake speed. These facts play an important role in the future definition of timing parameters and selective cell uptake control in order to achieve an effective therapy.
authors.affiliation: Inorganic and Bioinorganic Chemistry Department, Universidad Complutense de Madrid, 28040, Spain. mvila@farm.ucm.es
authors.forename: M
authors.initials: M
authors.lastname: Vila
authors.forename: M T
authors.initials: MT
authors.lastname: Portolés
authors.forename: P A A P
authors.initials: PA
authors.lastname: Marques
authors.forename: M J
authors.initials: MJ
authors.lastname: Feito
authors.forename: M C
authors.initials: MC
authors.lastname: Matesanz
authors.forename: C
authors.initials: C
authors.lastname: Ramírez-Santillán
authors.forename: G
authors.initials: G
authors.lastname: Gonçalves
authors.forename: S M A
authors.initials: SM
authors.lastname: Cruz
authors.forename: A
authors.initials: A
authors.lastname: Nieto
authors.forename: M
authors.initials: M
authors.lastname: Vallet-Regi
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006108:Graphite / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D006651:Histocytochemistry; D006801:Humans; D051379:Mice; D018613:Microscopy, Confocal; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010087:Oxides / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology
chemical_list: D010087:Oxides; D011092:Polyethylene Glycols; D006108:Graphite