title: An ascorbic acid sensor based on protein-modified Au nanoclusters.
abstract: A simple fluorescence sensor for sensitive turn-off detection of ascorbic acid (AA) was developed by using protein-modified Au nanoclusters in aqueous media. The sensing mechanism is originated from the oxidation state change of Au nanoclusters controlled by AA. Under optimal experimental conditions, a good linear relationship between the relative fluorescence quenching intensity and the concentration of AA can be obtained in the range of 1.5 to 10 μM, with a detection limit as low as 0.2 μM. The proposed method is simple, efficient and reliable for monitoring of AA in some biological samples.
authors.affiliation: College of Chemistry, Analytical & Testing Center, Sichuan University, Chengdu, Sichuan 610064, China.
authors.forename: Xianxiang
authors.initials: X
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Wu
authors.forename: Xiandeng
authors.initials: X
authors.lastname: Hou
authors.forename: Yi
authors.initials: Y
authors.lastname: Lv
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000097:blood*; D015374:Biosensing Techniques / Q000379:methods*; D001774:Blood Chemical Analysis; D002417:Cattle; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D012710:Serum Albumin, Bovine; D006046:Gold; D001205:Ascorbic Acid