title: Efficiency of different Agrobacterium rhizogenes strains on hairy roots induction in Solanum mammosum.
abstract: This article presents the abilities and efficiencies of five different strains of Agrobacterium rhizogenes (strain ATCC 31798, ATCC 43057, AR12, A4 and A13) to induce hairy roots on Solanum mammosum through genetic transformation. There is significant difference in the transformation efficiency (average number of days of hairy root induction) and transformation frequency for all strains of A. rhizogenes (P < 0.05). Both A. rhizogenes strain AR12 and A13 were able to induce hairy root at 6 days of co-cultivation, which were the fastest among those tested. However, the transformation frequencies of all five strains were below 30 %, with A. rhizogenes strain A4 and A13 showing the highest, which were 21.41 ± 10.60 % and 21.43 ± 8.13 % respectively. Subsequently, the cultures for five different hairy root lines generated by five different strains of bacteria were established. However, different hairy root lines showed different growth index under the same culture condition, with the hairy root lines induced by A. rhizogenes strain ATCC 31798 exhibited largest increase in fresh biomass at 45 days of culture under 16 h light/8 h dark photoperiod in half-strength MS medium. The slowest growing hairy root line, which was previously induced by A. rhizogenes strain A13, when cultured in optimized half-strength MS medium containing 1.5 times the standard amount of ammonium nitrate and potassium nitrate and 5 % (w/v) sucrose, had exhibited improvement in growth index, that is, the fresh biomass was almost double as compared to its initial growth in unmodified half-strength MS medium.
authors.affiliation: Laboratory of Natural Products, Institute of BioScience, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: Chai Theam
authors.initials: CT
authors.lastname: Ooi
authors.forename: Ahmad
authors.initials: A
authors.lastname: Syahida
authors.forename: Johnson
authors.initials: J
authors.lastname: Stanslas
authors.forename: Mahmood
authors.initials: M
authors.lastname: Maziah
mesh_terms: D060054:Agrobacterium / Q000145:classification / Q000235:genetics*; D018533:Biomass; D001709:Biotechnology / Q000379:methods; D003470:Culture Media; D018517:Plant Roots / Q000254:growth & development* / Q000382:microbiology*; D032322:Solanum / Q000254:growth & development / Q000382:microbiology*; D014170:Transformation, Genetic
chemical_list: D003470:Culture Media