title: Differential expression of neurotensin and specific receptors, NTSR1 and NTSR2, in normal and malignant human B lymphocytes.
abstract: Neurotensin, a neuropeptide growth factor, and its two specific neurotensin receptors, NTSR1 and NTSR2, were shown to be expressed by human B cell lines. Another NTSR, sortilin, which is common to neurotensin and neurotrophins, was also detected as we have previously described. Neurotensin was functional in B cell lines; it induced their proliferation and inhibited apoptosis induced by serum deprivation or Fas activation. Quantitative study of gene expression in two malignant B cell diseases showed that NTSR2 was overexpressed, NTSR1 decreased, and neurotensin was unexpressed in B cell leukemia patient's cells, as compared with healthy B cells. However, these expressions did not significantly change in large diffuse B cell lymphoma lymph nodes compared with benign ones. This study points out that neurotensin and its two specific receptors are expressed in human B lymphocytes. Such expressions were not described, and their relationship in B cell diseases, especially in chronic B cell leukemia, needs to be considered further in regard to these findings.
authors.affiliation: Department of Immunology, University of Limoges, Equipe Accueil 3842, 87025 Limoges, France.
authors.forename: Sofiane
authors.initials: S
authors.lastname: Saada
authors.forename: Pierre
authors.initials: P
authors.lastname: Marget
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Fauchais
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Lise
authors.forename: Guillaume
authors.initials: G
authors.lastname: Chemin
authors.forename: Philippe
authors.initials: P
authors.lastname: Sindou
authors.forename: Clothilde
authors.initials: C
authors.lastname: Martel
authors.forename: Laurent
authors.initials: L
authors.lastname: Delpy
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Vidal
authors.forename: Arnaud
authors.initials: A
authors.lastname: Jaccard
authors.forename: Danielle
authors.initials: D
authors.lastname: Troutaud
authors.forename: Fabrice
authors.initials: F
authors.lastname: Lalloué
authors.forename: Marie-Odile
authors.initials: MO
authors.lastname: Jauberteau
mesh_terms: D017209:Apoptosis; D001402:B-Lymphocytes / Q000378:metabolism* / Q000473:pathology; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D009496:Neurotensin / Q000235:genetics* / Q000378:metabolism; D009928:Organ Specificity; D061251:Primary Cell Culture; D018028:Receptors, Neurotensin / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism
chemical_list: C491901:FAS protein, human; C490985:NTSR2 protein, human; D018028:Receptors, Neurotensin; D019014:fas Receptor; C114036:neurotensin type 1 receptor; D009496:Neurotensin