title: PDLA/PLLA and PDLA/PCL nanofibers with a chitosan-based hydrogel in composite scaffolds for tissue engineered cartilage.
abstract: Osteoarthritis (OA) is the most prevalent musculoskeletal disease in humans, causing pain, loss of joint motility and function, and severely reducing the standard of living of patients. Cartilage tissue engineering attempts to repair the damaged tissue of individuals suffering from OA by providing mechanical support to the joint as new tissue regenerates. The aim of this study was to create composite three dimensional scaffolds comprised of electrospun poly(D,L-lactide)/poly(L-lactide) (PDLA/PLLA) or poly(D,L-lactide)/polycaprolactone (PDLA/PCL) with salt leached pores and an embedded chitosan hydrogel to determine the potential of these scaffolds for cartilage tissue engineering. PDLA/PLLA-hydrogel scaffolds displayed the largest compressive moduli followed by PDLA/PCL-hydrogel scaffolds. Dynamic mechanical tests showed that the PDLA/PLLA scaffolds had no appreciable recovery while PDLA/PCL scaffolds did exhibit some recovery. Primary canine chondrocytes produced both collagen type II and proteoglycans (primary components of extracellular matrix in cartilage) while being cultured on scaffolds composed of electrospun PDLA/PCL. As a result, a composite electrospun embedded hydrogel scaffold shows promise for treating individuals suffering from OA.
authors.affiliation: Virginia Tech-Wake Forest School of Biomedical Engineering and Sciences, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, 24061.
authors.forename: L D
authors.initials: LD
authors.lastname: Wright
authors.forename: K D
authors.initials: KD
authors.lastname: McKeon-Fischer
authors.forename: Z
authors.initials: Z
authors.lastname: Cui
authors.forename: L S
authors.initials: LS
authors.lastname: Nair
authors.forename: J W
authors.initials: JW
authors.lastname: Freeman
mesh_terms: D002356:Cartilage / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D020100:Hydrogels; D008422:Materials Testing; D057139:Nanofibers; D011108:Polymers / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D020100:Hydrogels; D011108:Polymers; D048271:Chitosan
keywords: cartilage; chitosan; electrospinning; hydrogel; polyester; scaffold; tissue engineering