title: Diversity of 'benzenetriol dioxygenase' involved in p-nitrophenol degradation in soil bacteria.
abstract: Ring hydroxylating dioxygenases (RHDOs) are one of the most important classes of enzymes featuring in the microbial metabolism of several xenobiotic aromatic compounds. One such RHDO is benzenetriol dioxygenase (BtD) which constitutes the metabolic machinery of microbial degradation of several mono- phenolic and biphenolic compounds including nitrophenols. Assessment of the natural diversity of benzenetriol dioxygenase (btd) gene sequence is of great significance from basic as well as applied study point of view. In the present study we have evaluated the gene sequence variations amongst the partial btd genes that were retrieved from microorganisms enriched for PNP degradation from pesticide contaminated agriculture soils. The gene sequence analysis was also supplemented with an in silico restriction digestion analysis. Furthermore, a phylogenetic analysis based on the deduced amino acid sequence(s) was performed wherein the evolutionary relatedness of BtD enzyme with similar aromatic dioxygenases was determined. The results obtained in this study indicated that this enzyme has probably undergone evolutionary divergence which largely corroborated with the taxonomic ranks of the host microorganisms.
authors.affiliation: Institute of Microbial Technology, Sector 39A, Chandigarh, India.
authors.forename: Debarati
authors.initials: D
authors.lastname: Paul
authors.forename: Neha
authors.initials: N
authors.lastname: Rastogi
authors.forename: Ulrich
authors.initials: U
authors.lastname: Krauss
authors.forename: Michael
authors.initials: M
authors.lastname: Schlomann
authors.forename: Gunjan
authors.initials: G
authors.lastname: Pandey
authors.forename: Janmejay
authors.initials: J
authors.lastname: Pandey
authors.forename: Anuradha
authors.initials: A
authors.lastname: Ghosh
authors.forename: Rakesh K
authors.initials: RK
authors.lastname: Jain
keywords: Benzenetriol dioxygenase; Phylogenetic analysis; p-Nitrophenol