title: Feeder-free culture of human embryonic stem cell line BG01V/hOG using magnetic field-magnetic nanoparticles system.
abstract: OBJECTIVE
  Human embryonic stem (hES) cells are useful tools for regenerative medicine. Maintaining hES cells for research and clinical purposes remains a challenge. The hES cells have typically been grown on a mouse or human cell feeder layer, but these methods harbor potential health problems for the recipient. A culture system using magnetic field and iron oxide nanoparticles were previously demonstrated to sustain mouse embryonic stem cells in vitro. Now, by using the BG01v/hOG cell line, we could assess the effect of this culture system on the stemness of an embryonic stem cell of human origin.
  
  
  METHODS
  Using a variant hES cell line, BG01V/hOG, expressing an emerald green fluorescent protein (EmGFP), we grown these cells in the presence of serum-free medium supplemented with magnetic nanoparticles functionalized with citrate. The cells were positioned over a circular magnet (4000 Gauss) and monitored daily by fluorescence microscopy.
  
  
  RESULTS
  We discovered that hES cells can proliferate when labeled with magnetic nanoparticles and in the presence of a magnetic field without losing pluripotency.
  
  
  CONCLUSIONS
  These results establish an alternative method for maintaining hES cells which would minimize health concerns as well as label cells for subsequent clinical tracking.
authors.affiliation: Department of Biochemistry and Molecular Biology, Biological Sciences Institute, Federal University of Goiás, Goiânia, Goiás, Brazil.
authors.forename: Erika Regina Leal de
authors.initials: ER
authors.lastname: Freitas
authors.forename: Regiane Lopes Dos
authors.initials: RL
authors.lastname: Santos
authors.forename: Emilia Celma de Oliveira
authors.initials: EC
authors.lastname: Lima
authors.forename: Lidia Andreu
authors.initials: LA
authors.lastname: Guillo
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D019343:Citric Acid / Q000737:chemistry; D016895:Culture Media, Serum-Free; D053595:Embryonic Stem Cells / Q000378:metabolism*; D005290:Ferric Compounds / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D008856:Microscopy, Fluorescence; D039904:Pluripotent Stem Cells / Q000378:metabolism*
chemical_list: D016895:Culture Media, Serum-Free; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D049452:Green Fluorescent Proteins; C000499:ferric oxide; D019343:Citric Acid