title: Candida ecuadorensis sp. nov., an ascomycetous yeast species found in two separate regions of Ecuador.
abstract: In the course of an on-going study aimed at cataloguing the natural yeast biodiversity found in Ecuador, two strains (CLQCA 13-025 and CLQCA 20-004(T)) were isolated from samples of cow manure and rotten wood collected in two separate provinces of the country (Orellana and Bolívar). These strains were found to represent a novel yeast species based on the sequences of their D1/D2 domain of the large-subunit (LSU) rRNA gene and their physiological characteristics. Phylogenetic analysis based on LSU D1/D2 sequences revealed this novel species to belong to the Metschnikowia clade and to be most closely related to Candida suratensis, a species recently discovered in a mangrove forest in Thailand. The species name of Candida ecuadorensis sp. nov. is proposed to accommodate these strains, with strain CLQCA 20-004(T) (=CBS 12653(T) = NCYC 3782(T)) designated as the type strain.
authors.affiliation: National Collection of Yeast Cultures (NCYC), Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: James
authors.affiliation: Colección de Levaduras Quito Católica (CLQCA), Centro Neotropical para Investigación de la Biomasa, Pontificia Universidad Católica del Ecuador, Escuela de Ciencias Biológicas, Quito, Ecuador.
authors.forename: Enrique Javier
authors.initials: EJ
authors.lastname: Carvajal Barriga
authors.affiliation: Colección de Levaduras Quito Católica (CLQCA), Centro Neotropical para Investigación de la Biomasa, Pontificia Universidad Católica del Ecuador, Escuela de Ciencias Biológicas, Quito, Ecuador.
authors.forename: Patricia Portero
authors.initials: PP
authors.lastname: Barahona
authors.affiliation: Imaging and Microscopy Group (IMG), Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK.
authors.forename: Kathryn
authors.initials: K
authors.lastname: Cross
authors.affiliation: National Collection of Yeast Cultures (NCYC), Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Bond
authors.affiliation: National Collection of Yeast Cultures (NCYC), Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK.
authors.forename: Ian N
authors.initials: IN
authors.lastname: Roberts
mesh_terms: D000818:Animals; D044822:Biodiversity; D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002417:Cattle; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D004484:Ecuador; D008372:Manure / Q000382:microbiology*; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014934:Wood / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D008372:Manure