title: Antigen processing and presentation by dendritic cells is independent of coronin 1.
abstract: Coronin 1, which is a member of the evolutionary conserved coronin protein family that is highly expressed in all leukocytes is involved in the activation of the Ca(2+)/calcineurin signaling pathway following cell surface stimulation in T cells, B cells as well as macrophages. Mice deficient for coronin 1 have strongly reduced peripheral T cell numbers as a result of a lack of pro-survival signals for naïve T cells. Whether or not impaired antigen processing and presentation in the absence of coronin 1 expression contributes to this reduction of T cell numbers is unknown. We here show that coronin 1-deficient bone marrow-derived dendritic cells develop normally, and that wild type and coronin 1-deficient dendritic cells were equally able to induce antigen-specific proliferation of T cells. Furthermore, upon immunization, in vivo proliferation of adoptively transferred antigen-specific T cells was comparable in wild type and coronin 1-deficient mice. Finally, infection of wild type and coronin 1-deficient dendritic cells with an ovalbumin-expressing Listeria monocytogenes strain induced comparable levels of ovalbumin-specific T cells responses. Together these results suggest that coronin 1 is dispensable for antigen processing and presentation by dendritic cells.
authors.affiliation: Biozentrum, University of Basel, Klingelbergstrasse 50, CH 4056 Basel, Switzerland.
authors.forename: Katrin
authors.initials: K
authors.lastname: Westritschnig
authors.forename: Somdeb
authors.initials: S
authors.lastname: BoseDasgupta
authors.forename: Vincent
authors.initials: V
authors.lastname: Tchang
authors.forename: Kerstin
authors.initials: K
authors.lastname: Siegmund
authors.forename: Jean
authors.initials: J
authors.lastname: Pieters
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D017951:Antigen Presentation / Q000235:genetics / Q000276:immunology*; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000276:immunology*; D008089:Listeria monocytogenes / Q000276:immunology; D018655:Lymphocyte Count; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008840:Microfilament Proteins / Q000172:deficiency / Q000235:genetics* / Q000276:immunology; D010047:Ovalbumin / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology / Q000637:transplantation
chemical_list: D008840:Microfilament Proteins; D011994:Recombinant Proteins; C072061:coronin proteins; D010047:Ovalbumin