title: Bilateral ovariectomy in young rats: what happens in their livers during cecal ligation and puncture induced sepsis?
abstract: OBJECTIVE
  Pediatric ovarian masses comprise a heterogeneous group of benign and malignant lesions. Surgical methods consist of emergency or programmed surgery with tumoral resection and uni/bilateral oophorectomy or salpingo-oophorectomy. We examined whether bilateral ovariectomy (OVX) worsens liver injury during the onset of cecal ligation and puncture (CLP)-induced sepsis in rats.
  
  
  METHODS
  The rat groups were: sham, bilateral-OVX, sepsis, and OVX-sepsis.
  
  
  METHODS
  After OVX operation, rats were allowed to recover for 12 weeks. At the end of recovery, CLP was applied 16 hours after sepsis induction.
  
  
  RESULTS
  There was a significant difference in the numerical density of hepatocytes only between the sepsis and the OVX-sepsis groups. Serum ALT and AST were increased significantly in the OVX-sepsis group. NF-κB activation after OVX increased after induction of sepsis. OVX-sepsis group showed marked thrombosis in portal vein branches and the central vein, degeneration in the bile ducts, and widespread ischemic areas in liver sections. Intra-inflammatory cell invasion was observed in both the portal and intrasinusoidal areas.
  
  
  CONCLUSIONS
  This study indicates that increases in liver NF-κB activity in ovariectomized rats following CLP-induced sepsis correlates with elevated levels of serum ALT and AST and with histopathologic changes in rat liver. Bilateral OVX therefore appears to play a role in the activation of NF-κB or in production of cytokines in liver cells. Thus, we provided novel insight into the effects of OVX on liver injury following CLP-induced sepsis.
authors.affiliation: Department of Pediatric Surgery, Ataturk University Faculty of Medicine, Erzurum, Turkey.
authors.forename: Akgun
authors.initials: A
authors.lastname: Oral
authors.forename: Deniz
authors.initials: D
authors.lastname: Unal
authors.forename: Zekai
authors.initials: Z
authors.lastname: Halici
authors.forename: Elif
authors.initials: E
authors.lastname: Cadirci
authors.forename: Ozlem
authors.initials: O
authors.lastname: Sengul
authors.forename: Seda
authors.initials: S
authors.lastname: Ozaltin
authors.forename: Cemal
authors.initials: C
authors.lastname: Gundogdu
authors.forename: Bunyami
authors.initials: B
authors.lastname: Unal
authors.forename: Sabri Selcuk
authors.initials: SS
authors.lastname: Atamanalp
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001652:Bile Ducts / Q000473:pathology; D002432:Cecum / Q000601:surgery; D004195:Disease Models, Animal; D005260:Female; D007511:Ischemia / Q000150:complications*; D008026:Ligation; D008099:Liver / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology*; D016328:NF-kappa B / Q000378:metabolism; D010052:Ovariectomy; D011169:Portal Vein; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000097:blood / Q000150:complications*; D013927:Thrombosis / Q000150:complications*
chemical_list: D016328:NF-kappa B; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase