title: Modulation of chondrocyte migration and aggregation by insulin-like growth factor-1 in cultured cartilage.
abstract: The effect of insulin-like growth factor-1 (IGF-1) on the behavior of rabbit chondrocytes in cultured collagen (CL) gels initially seeded with 2 × 10(5) cells/ml was examined. On day 5, the frequency of migrating cells cultured in presence of 100 ng IGF-1/ml was 0.04, which was 54 % of the frequency in IGF-1-free culture. The presence of IGF-1 caused an increase in the frequency of dividing cells from 0.09 to 0.13. These results suggest that IGF-1 suppressed the migration of chondrocytes in the CL gels while stimulating cell division in the initial culture phase. The proteolytic migration of cells was thought to be suppressed by the down-regulation of membrane type 1 matrix metalloproteinase by IGF-1. This contributed to the formation of aggregates with spherical-shaped cells that produced collagen type II.
authors.affiliation: Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka, Japan. masurina@cheng.es.osaka-u.ac.jp
authors.forename: Masrina Mohd
authors.initials: MM
authors.lastname: Nadzir
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kino-oka
authors.forename: Katsura
authors.initials: K
authors.lastname: Sugawara
authors.forename: Masahito
authors.initials: M
authors.lastname: Taya
mesh_terms: D000818:Animals; D002356:Cartilage / Q000166:cytology*; D002449:Cell Aggregation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D019902:Chondrocytes / Q000187:drug effects / Q000502:physiology*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D009924:Organ Culture Techniques; D011817:Rabbits
chemical_list: D007334:Insulin-Like Growth Factor I