title: Nano-formulation of paclitaxel by vitamin E succinate functionalized pluronic micelles for enhanced encapsulation, stability and cytotoxicity.
abstract: Vitamin E succinate (TOS) modified pluronic micelles (PF-TOS micelles) were prepared to be used as a vehicle for paclitaxel (PTX). The average size of resultant PTX-loaded PF-TOS micelles (PF-TOS-PTX-micelles) was about 58 nm. PF-TOS-PTX-micelles showed enhanced encapsulation efficiency and better stability as compared to the non-modified controls. Fluorescence microscopy and high performance liquid chromatography studies showed that PTX-loaded PF-TOS micelles had excellent cellular uptake ability by human glioma U87 cells. Cytotoxicity assay revealed that PTX-loaded PF-TOS micelles demonstrated higher anti-cancer activity to U87 cells than that of the non-modified PF micelles. Pharmacokinetic studies indicated the longer systemic circulation time and slower plasma elimination rate in PTX-loaded PF-TOS micelles than that of the non-modified controls. In vivo tissue distribution studies showed that PTX-loaded PF-TOS micelles were preferably accumulated in spleen and liver. Taken together, the results evidently indicated that PF-TOS micelles improve the hydrophobic chemotherapeutic drugs delivery by means of efficient encapsulation and stabilization for anti-tumor therapy.
authors.affiliation: Department of Biomedical Sciences, Texas Tech University Health Sciences Center, Paul L. Foster School of Medicine, El Paso, TX, USA.
authors.forename: Youhua
authors.initials: Y
authors.lastname: Tao
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Han
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Wang
authors.forename: Huanyu
authors.initials: H
authors.lastname: Dou
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004337:Drug Carriers / Q000009:adverse effects / Q000737:chemistry*; D004355:Drug Stability; D006801:Humans; D051379:Mice; D008823:Micelles; D017239:Paclitaxel / Q000737:chemistry*; D019802:Succinic Acid / Q000737:chemistry*; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D008823:Micelles; D014810:Vitamin E; D019802:Succinic Acid; D017239:Paclitaxel