title: The influence of exogenous carbohydrate provision and pre-exercise alkalosis on the heat shock protein response to prolonged interval cycling.
abstract: The aim of this study was to observe the intracellular heat shock protein 72 (HSP72) and heme oxygenase-1 (HSP32) response to prolonged interval cycling following the ingestion of carbohydrates (CHO) and sodium bicarbonate (NaHCO(3)). Six recreationally active males (mean ± SD; age 23.2 ± 2.9 years, height 179.5 ± 5.5 cm, body mass 76.5 ± 6.8 kg, and peak power output 315 ± 36 W) volunteered to complete a 90 min interval cycling exercise on four occasions. The trials were completed in a random and blinded manner following ingestion of either: placebo and an artificial sweetener (P-P), NaHCO(3) and sweetener (B-P), placebo and CHO (P-CHO), and NaHCO(3) and CHO (B-CHO). Both HSP72 and HSP32 were significantly increased in monocytes and lymphocytes from 45 min post-exercise (p ≤ 0.039), with strong relationships between both cell types (HSP72, r = 0.83; HSP32, r = 0.89). Exogenous CHO had no influence on either HSP72 or HSP32, but the ingestion of NaHCO(3) significantly attenuated HSP32 in monocytes and lymphocytes (p ≤ 0.042). In conclusion, the intracellular stress protein response to 90 min interval exercise is closely related in monocytes and lymphocytes, and HSP32 appears to be attenuated with a pre-exercise alkalosis.
authors.affiliation: Department of Sport, Health and Exercise Science, University of Hull, Hull HU6 7RX, UK.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Peart
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Kirk
authors.forename: Leigh A
authors.initials: LA
authors.lastname: Madden
authors.forename: Jason C
authors.initials: JC
authors.lastname: Siegler
authors.forename: Rebecca V
authors.initials: RV
authors.lastname: Vince
mesh_terms: D000328:Adult; D000471:Alkalosis / Q000235:genetics / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D004435:Eating; D015444:Exercise / Q000502:physiology*; D050884:HSP72 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D009000:Monocytes / Q000378:metabolism; D017693:Sodium Bicarbonate / Q000378:metabolism; D013312:Stress, Physiological; D055815:Young Adult
chemical_list: D050884:HSP72 Heat-Shock Proteins; D017693:Sodium Bicarbonate; D051547:Heme Oxygenase-1