title: Molecularly imprinted electrochemical luminescence sensor based on signal amplification for selective determination of trace gibberellin A3.
abstract: A new molecularly imprinted electrochemical luminescence (MIP-ECL) sensor was developed for Gibberellin A3 (GA3) determination. This sensor is based on competitive binding between the GA3 and the Rhodamine B (RhB)-labeled GA3 (RhB-GA3) to the MIP film. After the competitive binding, the residual RhB-GA3 on the MIP was electro-oxidized to produce RhB oxide, which could greatly amplify the weak electrochemiluminescence (ECL) signal of luminol. The ECL intensity decreased when the RhB-GA3 was replaced by GA3 molecules in the samples. Accordingly, GA3 was determined in the concentration range from 1 × 10(-11) to 3 × 10(-9) mol/L with a detection limit of 3.45 × 10(-12) mol/L. The sensor shows high sensitivity and selectivity, wide response range, good accuracy, and fast response. Beer samples were assayed by using the sensors, and the recoveries ranging from 96.0% to 103.2% were obtained.
authors.affiliation: College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi 541004, China. likianping@263.net
authors.forename: Jianping
authors.initials: J
authors.lastname: Li
authors.forename: Shuhuai
authors.initials: S
authors.lastname: Li
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Wei
authors.forename: Huilin
authors.initials: H
authors.lastname: Tao
authors.forename: Hongcheng
authors.initials: H
authors.lastname: Pan
mesh_terms: D001515:Beer / Q000032:analysis; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D004563:Electrochemistry; D005875:Gibberellins / Q000032:analysis* / Q000737:chemistry; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry; D054802:Molecular Imprinting; D010655:Phenylenediamines / Q000737:chemistry; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis; D015203:Reproducibility of Results; D012235:Rhodamines / Q000737:chemistry
chemical_list: D005875:Gibberellins; D010655:Phenylenediamines; D011108:Polymers; D012235:Rhodamines; D008165:Luminol; C007842:gibberellic acid; C029773:rhodamine B