title: Changes in activity of proline-specific peptidases in rat model for dementia of Alzheimer's type.
abstract: We studied the role of proline-specific peptidases in the pathogenesis of Alzheimer's disease. Testing of conditioned passive avoidance 24 h after learning showed that chronic administration of scopolamine to rats 4-fold reduced the latency of entry into the dark chamber in comparison with controls (intact animals). Activity of prolyl endopeptidase was significantly higher than in the controls in both the cortex and hippocampus. Changes in dipeptidyl peptidase IV activity were observed only in the cortex. Injection of AF-64A toxin into Meynert nucleus basalis reduced the latency of entry into the dark compartment by 75% in comparison with that in sham-operated and intact controls. Prolyl endopeptidase activity was reduced in the frontal cortex and hippocampus, but not in hypothalamus. Changes in dipeptidyl peptidase IV activity were detected only in the frontal cortex.
authors.affiliation: PK Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences, VV Zakusov Institute of Pharmacology, Russian Academy of Medical Sciences, Moscow, Russia. ganazarova@rambler.ru
authors.forename: G A
authors.initials: GA
authors.lastname: Nazarova
authors.forename: K N
authors.initials: KN
authors.lastname: Kolyasnikova
authors.forename: N N
authors.initials: NN
authors.lastname: Zolotov
mesh_terms: D000544:Alzheimer Disease / Q000201:enzymology*; D000647:Amnesia / Q000139:chemically induced*; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects*; D001388:Aziridines / Q000633:toxicity; D002540:Cerebral Cortex / Q000187:drug effects; D002794:Choline / Q000031:analogs & derivatives / Q000633:toxicity; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects; D008297:Male; D000085822:Prolyl Oligopeptidases; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time / Q000187:drug effects; D012601:Scopolamine / Q000633:toxicity; D012697:Serine Endopeptidases / Q000378:metabolism*; D018709:Statistics, Nonparametric
chemical_list: D001388:Aziridines; C044894:ethylcholine aziridinium; D012601:Scopolamine; D018819:Dipeptidyl Peptidase 4; D012697:Serine Endopeptidases; D000085822:Prolyl Oligopeptidases; D002794:Choline