title: A versatile monoclonal antibody specific to human SERPINB5.
abstract: Maspin (SERPINB5) is a member of the Clade B subgroup of the large superfamily of serine protease inhibitors. It is proposed that maspin is a tumor suppressor; however, its molecular role remains to be elucidated. Here we report the characterization of a mouse monoclonal antibody directed against human maspin. This antibody, 16F7, recognizes maspin in both its native and denatured form, unlike several other commercial antibodies tested in this study. It will be a useful and versatile tool for future analyses of the biological function of maspin.
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Victoria, Australia.
authors.forename: Sonia S Y
authors.initials: SS
authors.lastname: Teoh
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Gail P
authors.initials: GP
authors.lastname: Risbridger
authors.forename: James C
authors.initials: JC
authors.lastname: Whisstock
authors.forename: Phillip I
authors.initials: PI
authors.lastname: Bird
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D019556:COS Cells; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D051379:Mice; D011485:Protein Binding; D011487:Protein Conformation; D011489:Protein Denaturation; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology; D015843:Serpins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins; C085344:SERPIN-B5; D015843:Serpins