title: Sim3 shares some common roles with the histone chaperone Asf1 in fission yeast.
abstract: An H3/H4 histone chaperone, Asf1, plays an essential role in maintaining genomic stability in many species, including fission yeast. Here, we showed that overexpression of a CENP-A chaperone Sim3 suppressed the temperature sensitive phenotype of asf1-33 and asf1-30 mutants and the defect in chromatin structure, and prevented the accumulation of DNA damage in asf1-33 mutants at high temperatures. Furthermore, asf1-33 and Δsim3 were synthetic lethal. Consistent with this, shutdown of sim3 expression in asf1-33 Δsim3 double mutants that contained extragenic sim3 resulted in growth retardation. In addition, the Δsim3 mutant displayed sensitivity to thiabendazol and hydroxyurea, which suggests that Sim3 plays a general role in maintaining chromatin structure. Our results suggest a possibility that Sim3 functions as a histone chaperone.
authors.affiliation: Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University, Matsue 690-8504, Japan.
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Tanae
authors.forename: Tomitaka
authors.initials: T
authors.lastname: Horiuchi
authors.forename: Takuya
authors.initials: T
authors.lastname: Yamakawa
authors.forename: Yuzy
authors.initials: Y
authors.lastname: Matsuo
authors.forename: Makoto
authors.initials: M
authors.lastname: Kawamukai
mesh_terms: D002843:Chromatin / Q000187:drug effects / Q000378:metabolism; D006918:Hydroxyurea / Q000494:pharmacology; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D013827:Thiabendazole / Q000494:pharmacology
chemical_list: D002843:Chromatin; C524040:Cia1 protein, S pombe; D018832:Molecular Chaperones; D009687:Nuclear Proteins; D029702:Schizosaccharomyces pombe Proteins; C526321:Sim3 protein, S pombe; D013827:Thiabendazole; D006918:Hydroxyurea