title: The effects of the 11 kDa protein and the putative X protein on the p6 promoter activity of Parvovirus B19 in Hela cells.
abstract: Human parvovirus B19 (B19) is a small nonenveloped icosahedral virus with a single-stranded, linear 5.6 kb DNA genome. The p6 promoter, at map unit 6 of the viral genome, controls the expression of all B19 transcripts. Some previous reports revealed that this promoter is transactivated by NS1 protein. In an attempt to investigate the roles of other small viral proteins in the control of the p6 promoter activity, various truncated promoter/reporter constructs along with these nonstructural protein expression vectors were introduced into Hela cells. The results showed that the putative X protein upregulated the activity of p6 promoter significantly, but that the 11 kDa protein did not. Furthermore, the possible responsive DNA elements for X protein were identified to be located primarily between nt 265 and 343 of the p6 promoter region. In addition, we observed that deletion of the potential ATF/CREB binding sites located in 5' terminal nucleotide influenced the activity of p6 promoter significantly.
authors.affiliation: College of Life Sciences, Central China Normal University, 152, Luoyu Road, Wuhan, Hubei, People's Republic of China.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Dong
authors.forename: Yu
authors.initials: Y
authors.lastname: Huang
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Yang
authors.forename: Kaiyu
authors.initials: K
authors.lastname: Liu
authors.forename: Peter
authors.initials: P
authors.lastname: Tijssen
authors.forename: Jianxin
authors.initials: J
authors.lastname: Peng
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
mesh_terms: D004847:Epithelial Cells / Q000821:virology*; D015967:Gene Expression Regulation, Viral; D006367:HeLa Cells; D006801:Humans; D016732:Parvovirus B19, Human / Q000472:pathogenicity*; D011401:Promoter Regions, Genetic; D017361:Viral Nonstructural Proteins / Q000378:metabolism*
chemical_list: C512342:11-kDa protein, human parvovirus B19; D017361:Viral Nonstructural Proteins