title: The design of microfluidic affinity chromatography systems for the separation of bioanalytes.
abstract: The analytical (numerical) design of planar microfluidic affinity chromatography devices, which consist of multiple separation lanes and multiple, different surface-immobilised receptor patterns in each lane, is described. The model is based on the analytical solution of the transport-reaction equations in microfluidic systems of low Gratz number and for injection of small analyte plugs. The results reveal a simple approach for the design of microfluidic affinity chromatography devices tailored to the separation of bioanalytes, where receptors with high binding affinity are available. These devices have been designed for bioanalytical applications in mind, most notably for the proteomics field; the results are illustrated with an example using β-Amyloid binding peptides.
authors.affiliation: Optoelectronics Research Centre, University of Southampton, Highfield, Hampshire SO17 1BJ, UK.
authors.forename: Daniel
authors.initials: D
authors.lastname: Friedrich
authors.forename: Colin P
authors.initials: CP
authors.lastname: Please
authors.forename: Tracy
authors.initials: T
authors.lastname: Melvin
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D044085:Microfluidics / Q000379:methods*; D011485:Protein Binding
chemical_list: D016229:Amyloid beta-Peptides