title: Innate aminoglycoside resistance of Achromobacter xylosoxidans is due to AxyXY-OprZ, an RND-type multidrug efflux pump.
abstract: Achromobacter xylosoxidans is an innately multidrug-resistant pathogen which is emerging in cystic fibrosis (CF) patients. We characterized a new resistance-nodulation-cell division (RND)-type multidrug efflux pump, AxyXY-OprZ. This system is responsible for the intrinsic high-level resistance of A. xylosoxidans to aminoglycosides (tobramycin, amikacin, and gentamicin). Furthermore, it can extrude cefepime, carbapenems, some fluoroquinolones, tetracyclines, and erythromycin. Some of the AxyXY-OprZ substrates are major components widely used to treat pulmonary infections in CF patients.
authors.affiliation: Department of Bacteriology, University Hospital of Dijon, Dijon, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Bador
authors.forename: Lucie
authors.initials: L
authors.lastname: Amoureux
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Blanc
authors.forename: Catherine
authors.initials: C
authors.lastname: Neuwirth
mesh_terms: D042441:Achromobacter denitrificans / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D000617:Aminoglycosides / Q000494:pharmacology* / Q000627:therapeutic use; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use; D015780:Carbapenems / Q000494:pharmacology / Q000627:therapeutic use; D003550:Cystic Fibrosis / Q000188:drug therapy / Q000382:microbiology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D024841:Fluoroquinolones / Q000494:pharmacology / Q000627:therapeutic use; D019450:Genes, MDR; D016680:Genome, Bacterial; D016905:Gram-Negative Bacterial Infections / Q000188:drug therapy / Q000382:microbiology; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D026901:Membrane Transport Proteins / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D012141:Respiratory Tract Infections / Q000188:drug therapy / Q000382:microbiology; D017422:Sequence Analysis, DNA
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D015780:Carbapenems; D024841:Fluoroquinolones; D026901:Membrane Transport Proteins