title: The -514C/T Polymorphism of Hepatic Lipase Gene among Iranian Patients with Coronary Heart Disease.
abstract: BACKGROUND
  The T allele of the hepatic lipase (HL) C-514T polymorphism was previously found to be associated with lower plasma HL activity. Here, we examined the association between this polymorphism and plasma HDL-cholesterol concentrations in patients with coronary arteries stenosis.
  
  
  METHODS
  We studied 342 subjects undergoing coronary angiography in two groups of non CAD (n=146) and CAD (n=196). -514C→T polymorphism was determined using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).
  
  
  RESULTS
  After adjustment for age, smoking and body mass index, HDL-cholesterol concentrations were significantly higher in men with the C/T&T/T genotype than those with the C/C genotype(mean 38.6 and 34.7 respectively P=0.01). The frequency of T allele in non CAD was 0.136 and 0.226 in female and male respectively and 0.170 and 0.223 for female and male in CAD subjects. There was no difference in T allele frequency in CAD and none CAD groups in male and female (P=0.466 and 0.722 respectively).
  
  
  CONCLUSIONS
  -514C→T of LIPC gene have a positive effect on HDL-C concentration especially in male gender. However, no difference was determined in frequency of T allele between CAD and normal arteries subjects.
authors.affiliation: Dept. of Biochemistry, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
authors.forename: K
authors.initials: K
authors.lastname: Ghatreh Samani
authors.forename: M
authors.initials: M
authors.lastname: Noori
authors.forename: M Rohbani
authors.initials: MR
authors.lastname: Nobar
authors.forename: M Hashemzadeh
authors.initials: MH
authors.lastname: Chaleshtory
authors.forename: E
authors.initials: E
authors.lastname: Farrokhi
authors.forename: M Darabi
authors.initials: MD
authors.lastname: Amin
keywords: Coronary artery stenosis; HDL-C; Hepatic lipase gene; Iran; T allele