title: Microbial evolution in vivo and in silico: methods and applications.
abstract: Microbial evolution has been extensively studied in the past fifty years, which has lead to seminal discoveries that have shaped our understanding of evolutionary forces and dynamics. It is only recently however, that transformative technologies and computational advances have enabled a larger in-scale and in-depth investigation of the genetic basis and mechanistic underpinnings of evolutionary adaptation. In this review we focus on the strengths and limitations of in vivo and in silico techniques for studying microbial evolution in the laboratory, and we discuss how these complementary approaches can be integrated in a unifying framework for elucidating microbial evolution.
authors.affiliation: Department of Computer Science, UC Davis Genome Center, University of California Davis, Davis, California 95616, USA.
authors.forename: Vadim
authors.initials: V
authors.lastname: Mozhayskiy
authors.forename: Ilias
authors.initials: I
authors.lastname: Tagkopoulos
mesh_terms: D001419:Bacteria / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D005075:Biological Evolution; D003198:Computer Simulation; D008957:Models, Genetic; D009154:Mutation / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins