title: Muscle and connective tissue progenitor populations show distinct Twist1 and Twist3 expression profiles during axolotl limb regeneration.
abstract: Limb regeneration involves re-establishing a limb development program from cells within adult tissues. Identifying molecular handles that provide insight into the relationship between cell differentiation status and cell lineage is an important step to study limb blastema cell formation. Here, using single cell PCR, focusing on newly isolated Twist1 sequences, we molecularly profile axolotl limb blastema cells using several progenitor cell markers. We link their molecular expression profile to their embryonic lineage via cell tracking experiments. We use in situ hybridization to determine the spatial localization and extent of overlap of different markers and cell types. Finally, we show by single cell PCR that the mature axolotl limb harbors a small but significant population of Twist1(+) cells.
authors.affiliation: Max-Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Kragl
authors.forename: Kathleen
authors.initials: K
authors.lastname: Roensch
authors.forename: Ina
authors.initials: I
authors.lastname: Nüsslein
authors.forename: Akira
authors.initials: A
authors.lastname: Tazaki
authors.forename: Yuka
authors.initials: Y
authors.lastname: Taniguchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tarui
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Hayashi
authors.forename: Kiyokazu
authors.initials: K
authors.lastname: Agata
authors.forename: Elly M
authors.initials: EM
authors.lastname: Tanaka
mesh_terms: D000558:Ambystoma mexicanum / Q000502:physiology*; D000818:Animals; D019070:Cell Lineage / Q000502:physiology; D003238:Connective Tissue / Q000378:metabolism*; D003239:Connective Tissue Cells / Q000378:metabolism; D005121:Extremities / Q000502:physiology*; D017403:In Situ Hybridization; D008648:Mesoderm / Q000166:cytology; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012038:Regeneration / Q000502:physiology*; D012867:Skin / Q000166:cytology; D013234:Stem Cells / Q000378:metabolism*; D059467:Transcriptome; D051879:Twist-Related Protein 1 / Q000378:metabolism*
chemical_list: D051879:Twist-Related Protein 1