title: Elemental mapping by electron energy loss spectroscopy in biology.
abstract: Over the past decades there have been significant advances in transmission electron microscopy for biological applications, including in energy filtering and spectrum imaging, which are techniques based on the principles of electron energy loss spectroscopy. These imaging modalities allow quantitative mapping of specific chemical elements with high sensitivity and spatial resolution. This chapter describes the experimental and computational procedures for elemental mapping in two dimensions as well as a more recent extension to three dimensions, which can reveal quantitative distributions of elements in cells on a macromolecular scale.
authors.affiliation: Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Aronova
authors.forename: Richard D
authors.initials: RD
authors.lastname: Leapman
mesh_terms: D000818:Animals; D001695:Biology / Q000379:methods*; D017173:Caenorhabditis elegans / Q000166:cytology; D004247:DNA / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology / Q000196:embryology; D004602:Elements; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism; D021621:Imaging, Three-Dimensional; D046529:Microscopy, Electron, Transmission / Q000295:instrumentation; D010758:Phosphorus / Q000378:metabolism; D046590:Spectroscopy, Electron Energy-Loss / Q000379:methods*; D013223:Statistics as Topic; D060168:Thymocytes / Q000166:cytology / Q000648:ultrastructure
chemical_list: D004602:Elements; D010758:Phosphorus; D004247:DNA