title: [Effect of mPGES-1 inhibitor MK886 on cell cycle of leukemia HL-60 cells].
abstract: To investigate the effect of a microsomal prostaglandin E synthase-1 (mPGES-1) inhibitor MK886 on cell cycle of the human acute myeloid leukemia HL-60 cells. HL-60 cells were treated with different concentration of MK886 (10, 25, 50 µmol/L) for 24 h. Flow cytometry, Western blot and ELISA were used to measure cell cycle, cyclin D1, mPGES-1, PGE(2), Akt, P-Akt and C-MYC. The results indicated that after treated with MK886, the percentage of HL-60 cells decreased in G(0)/G(1) phase and increased in S phase, and expressions of mPGES-1, cyclin D1, P-Akt and C-MYC and synthesis of PGE(2) decreased significantly. It is concluded that MK886 can arrest HL-60 cells in G(0)/G(1) phase, the mechanism of which is possibly associated to inhibition of mPGES-1 expression, reduction of PGE(2) synthesis, suppression of Akt phosphorylation and C-MYC expression, down-regulation of cyclin D1 expression.
authors.affiliation: Department of Internal Hematology, SUN Yat-Sen University, Guangzhou, China.
authors.forename: Yi-Qing
authors.initials: YQ
authors.lastname: Li
authors.forename: Song-Mei
authors.initials: SM
authors.lastname: Yin
authors.forename: Shuang-Feng
authors.initials: SF
authors.lastname: Xie
authors.forename: Xiu-Ju
authors.initials: XJ
authors.lastname: Wang
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Ma
authors.forename: Da-Nian
authors.initials: DN
authors.lastname: Nie
authors.forename: Yu-Dan
authors.initials: YD
authors.lastname: Wu
mesh_terms: D002453:Cell Cycle / Q000187:drug effects*; D018922:HL-60 Cells; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D019746:Intramolecular Oxidoreductases / Q000037:antagonists & inhibitors*; D007938:Leukemia / Q000378:metabolism / Q000473:pathology*; D000071876:Prostaglandin-E Synthases; D002722:Chlorobenzenes
chemical_list: D007211:Indoles; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; C060893:MK-886; D002722:Chlorobenzenes; C000605862:PTGES protein, human