title: [Effect of osteogenically and adipogenically differentiated bone mesenchymal stem cells from mouse on osteoclast formation].
abstract: This study was purposed to investigate the regulatory effects of differentiating mesenchymal stem cells (MSC) on osteoclast formation. The MSC from mouse compact bones were cultured and induced into osteoblasts and adipocytes for one week. To test their regulatory effect on osteoclastogenesis, osteogenically differentiated and adipogenically differentiated MSC were co-cultured with CD11b(+) monocytes and osteoclasts were identified with in situ tartrate-resistant acid phosphatase (TRAP) staining. The results showed that differentiated MSC supported osteoclastogenesis but the osteoclast supporting capacity of osteogenically differentiated MSC decreased as compared with undifferentiated MSC. More interestingly, the adipogenically differentiated MSC significantly promoted osteoclasts formation when co-cultured with monocytes. It is concluded that the regulatory effect of MSC on osteoclast formation has changed while they have differentiated into different types of cells. The findings indicate that MSC may exert alternative effect on osteoclastogenesis by differentiation to descendant cells.
authors.affiliation: Department of Cell Biology, Academy of Military Medical Sciences, Beijing, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhu
authors.forename: Yuan-Lin
authors.initials: YL
authors.lastname: Liu
authors.forename: Ji-De
authors.initials: JD
authors.lastname: Chen
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Yu-Xiao
authors.initials: YX
authors.lastname: Liu
authors.forename: Fen-Fen
authors.initials: FF
authors.lastname: Xu
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Jiang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ning
authors.initials: N
authors.lastname: Mao
mesh_terms: D050156:Adipogenesis; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000166:cytology; D010006:Osteoblasts / Q000166:cytology*; D010010:Osteoclasts / Q000166:cytology*