title: Characterization of Vibrio species isolated from freshwater fishes by ribotyping.
abstract: Three Vibrio species from the resident microflora of gastrointestinal tract of freshwater carps and prawns were isolated and confirmed biochemically as V. fluvialis from Cyprinus carpio/Labeo rohita; V. parahaemolyticus from Macrobrachium rosenbergii and V. harveyi from Macrobrachium malcomsoni. The genetic relationship among these Vibrio species was carried out by polymerase chain reaction (PCR) amplification of 16S rRNA gene followed by restriction digestion with Hae III, Bam HI and Pst I. Dendogram based on ribotyping showed the isolated Vibrios were differentiated into three clusters. V. harveyi was closely related to V. vulnificus (reference Microbial type Culture Collection (MTCC) strain) and distantly related to V. parahaemolyticus as well as V. fluvialis.
authors.affiliation: Microbiology Division, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, 751002 India.
authors.forename: P
authors.initials: P
authors.lastname: Mishra
authors.forename: M
authors.initials: M
authors.lastname: Samanta
authors.forename: S
authors.initials: S
authors.lastname: Mohanty
authors.forename: N K
authors.initials: NK
authors.lastname: Maiti
keywords: 16S rRNA gene; Freshwater Vibrios; Ribotyping