title: Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze.
abstract: An efficient protocol for organogenesis through leaves has been established for Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze, a highly valuable medicinal plant. The leaf explants produced microshoots on MS basal medium when fortified with cytokinins and auxins. A combination of 6-benzylaminopurine (BAP) at 0.5mg/l and naphthaleneacetic acid (NAA) at 0.2mg/l resulted in the induction of high frequency microshoots in 30 days. The microshoots were successfully subcultured for shoot elongation and eventually for rooting on MS medium supplemented with indole-3-butyric acid (IBA) at 0.5mg/l. The regenerated plantlets were hardened under greenhouse conditions and transferred to garden, resulting in a 90% survival rate.
authors.affiliation: Department of Plant Genetics, Institute of Plant Sciences, ARO, The Volcani Center, Bet-Dagan, 50250 Israel. a.mahesh05@gmail.com
authors.forename: Ayyavu
authors.initials: A
authors.lastname: Mahesh
authors.forename: Devarajan
authors.initials: D
authors.lastname: Thangadurai
authors.forename: Gabriel
authors.initials: G
authors.lastname: Melchias
mesh_terms: D019659:Asteraceae / Q000187:drug effects / Q000254:growth & development*; D001593:Benzyl Compounds / Q000494:pharmacology; D009280:Naphthaleneacetic Acids; D063246:Organogenesis, Plant / Q000187:drug effects / Q000502:physiology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development*; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development*; D011687:Purines / Q000494:pharmacology; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*
chemical_list: D001593:Benzyl Compounds; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; D011687:Purines; C480551:benzylaminopurine