title: Optimization of ligninolytic enzyme activity and production rate with Ceriporiopsis subvermispora for application in bioremediation by varying submerged media composition and growth immobilization support.
abstract: Response surface methodology (central composite design of experiments) was employed to simultaneously optimize enzyme production and productivities of two ligninolytic enzymes produced by Ceriporiopsis subvermispora. Concentrations of glucose, ammonium tartrate and Polysorbate 80 were varied to establish the optimal composition of liquid media (OLM), where the highest experimentally obtained activities and productivities were 41 U L(-1) and 16 U L(-1) day(-1) for laccase (Lac), and 193 U L(-1) and 80 U L(-1) day(-1) for manganese peroxidase (MnP). Considering culture growth in OLM on various types of immobilization support, the best results were obtained with 1 cm beech wood cubes (BWCM). Enzyme activities in culture filtrate were 152 U L(-1) for Lac and 58 U L(-1) for MnP, since the chemical composition of this immobilization material induced higher Lac activity. Lower enzyme activities were obtained with polyurethane foam. Culture filtrates of OLM and BWCM were applied for dye decolorization. Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role. Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM. The extent of decolorization after 14 h was 60% for both dyes.
authors.affiliation: Chair of Chemical, Biochemical and Environmental Engineering, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, 1000 Ljubljana, Slovenia.
authors.forename: Janja
authors.initials: J
authors.lastname: Babič
authors.affiliation: Laboratory of Catalysis and Chemical Reaction Engineering, National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia.
authors.forename: Blaž
authors.initials: B
authors.lastname: Likozar
authors.affiliation: Chair of Chemical, Biochemical and Environmental Engineering, Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, 1000 Ljubljana, Slovenia.
authors.forename: Aleksander
authors.initials: A
authors.lastname: Pavko
mesh_terms: D001673:Biodegradation, Environmental; D055453:Coriolaceae / Q000201:enzymology*; D057075:Enzyme Assays; D042845:Laccase / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism*
chemical_list: D008031:Lignin; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase
keywords: bioremediation; decolorization; enzyme production; immobilized culture; laccase; ligninolytic enzymes; manganese peroxidase; medium optimization; response surface methodology; submerged cultivation