title: Co-expression of Erns and E2 genes of classical swine fever virus by replication-defective recombinant adenovirus completely protects pigs against virulent challenge with classical swine fever virus.
abstract: The objective of this study was to construct a recombinant adenovirus for future CSFV vaccines used in the pig industry for the reduction of losses involved in CSF outbreaks. The Erns and E2 genes of classical swine fever virus (CSFV), which encode the two main protective glycoproteins from the "Shimen" strain of CSFV, were combined and inserted into the replication-defective human adenovirus type-5 and named the rAd-Erns-E2. Nine pigs were randomly assigned to three treatment groups (three pigs in each group) including the rAd-Erns-E2, hAd-CMV control and DMEM control. Intramuscular vaccination with 2×10(6) TCID(50) of the rAd-Erns-E2 was administered two times with an interval of 21 days. At 42 days post inoculation, pigs in all groups were challenged with a lethal dose of 1×10(3) TCID(50) CSFV "Shimen" strain. Observation of clinical signs was made and the existence of CSFV RNA was detected. Animals in the hAd-CMV and DMEM groups showed severe clinical CSF symptoms and were euthanized from 7 to 10 days after the challenge. However, no adverse clinical CSF signs were observed in vaccinated pigs after the administration of rAd-Erns-E2 and even after CSFV challenge. Neither CSFV RNA nor pathological changes were detected in the tissues of interest of the above vaccinated pigs. These results implied that the recombination adenovirus carrying the Erns-E2 genes could be used to prevent swine from classical swine fever.
authors.affiliation: Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China. sun_yongke@sina.com
authors.forename: Yongke
authors.initials: Y
authors.lastname: Sun
authors.forename: Yuai
authors.initials: Y
authors.lastname: Yang
authors.forename: Huanli
authors.initials: H
authors.lastname: Zheng
authors.forename: Dongmei
authors.initials: D
authors.lastname: Xi
authors.forename: Mingxing
authors.initials: M
authors.lastname: Lin
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Zhang
authors.forename: Linfu
authors.initials: L
authors.lastname: Yang
authors.forename: Yulin
authors.initials: Y
authors.lastname: Yan
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Chu
authors.forename: Baoliang
authors.initials: B
authors.lastname: Bi
mesh_terms: D000256:Adenoviridae; D000818:Animals; D006691:Classical Swine Fever / Q000517:prevention & control*; D006692:Classical Swine Fever Virus / Q000472:pathogenicity*; D015967:Gene Expression Regulation, Viral; D057809:HEK293 Cells; D006801:Humans; D013552:Swine; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014774:Virulence; D014779:Virus Replication / Q000235:genetics
chemical_list: D014764:Viral Proteins