title: Effect of the fungal immunomodulatory protein FIP-fve on airway inflammation and cytokine production in mouse asthma model.
abstract: The allergy is dependent on the balance between Th1 and Th2. The fungal immunodulatory protein (FIP-fve) was isolated from Flammulina velutipes. FIP-fve has been demonstrated to skew the response to Th1 cytokine production. We investigated whether oral administrations of FIP-fve inhibited allergen (OVA)-induced chronic airway inflammation in the mouse asthma model. After intranasal challenge with OVA, the airway inflammation and hyperresponsiveness were determined by bronchoalveolar lavage fluid (BALF) analysis and ELISA assay. Both pre-treated and post-treated with FIP-fve suppressed the airway hyperresponsiveness by methacholine challenge and significantly decreased the number of infiltrating inflammatory cells and Th2 cytokines in bronchoalveolar lavage fluid (BALF) and serum compared with the OVA sensitized mice. In addition, FIP-fve reduced OVA-specific IgE levels in serum. FIP-fve markedly alleviated the OVA-induced airway hyperresponsiveness (AHR) to inhaled methacholine. Based on lung histopathological studies using hematoxylin and Liu's staining, FIP-fve inhibited inflammatory cell infiltration compared with the OVA-sensitized mice. Oral FIP-fve had an anti-inflammatory effect on OVA-induced airway inflammations and might posses the potential for alternative therapy for allergic airway diseases.
authors.affiliation: Institute of Medicine, School of Public Health, Chung Shan Medical University, and Department of Pediatrics, Chung Shan Medical University Hospital, Taichung, Taiwan. tsz312@hotmail.com
authors.forename: Yu-Tzu
authors.initials: YT
authors.lastname: Lee
authors.forename: Shiuan-Shinn
authors.initials: SS
authors.lastname: Lee
authors.forename: Hai-Lun
authors.initials: HL
authors.lastname: Sun
authors.forename: Ko-Hsiu
authors.initials: KH
authors.lastname: Lu
authors.forename: Min-Sho
authors.initials: MS
authors.lastname: Ku
authors.forename: Ji-Nan
authors.initials: JN
authors.lastname: Sheu
authors.forename: Jiunn-Liang
authors.initials: JL
authors.lastname: Ko
authors.forename: Ko-Haung
authors.initials: KH
authors.lastname: Lue
mesh_terms: D000818:Animals; D001249:Asthma / Q000276:immunology* / Q000503:physiopathology; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D005656:Fungal Proteins / Q000494:pharmacology*; D007249:Inflammation / Q000276:immunology; D018836:Inflammation Mediators; D016210:Methacholine Chloride / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin; D012137:Respiratory System / Q000276:immunology* / Q000473:pathology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D016207:Cytokines; C547132:FIP-fve protein, Flammulina velutipes; D005656:Fungal Proteins; D018836:Inflammation Mediators; D016210:Methacholine Chloride; D010047:Ovalbumin