title: Dexamethasone inhibits U937 cell adhesion via the down-regulation of ROCK1 activity.
abstract: OBJECTIVE
  To explore the effect of dexamethasone (DEX) on monocyte adhesion function and its underlying mechanism.
  
  
  METHODS
  The effects of DEX and fasudil on adhesion of cultured U937 monocytes to human umbilical vein endothelial cells (HUVEC) following stimulation with phorbol myristate acetate (PMA) were studied; Changes in the Rho-associated coiled-coil protein kinase 1 (ROCK1) protein content and activity were evaluated.
  
  
  RESULTS
  DEX and fasudil significantly inhibited U937 cell adhesion rates under PMA stimulation and inhibited ROCK1 activity. Mifepristone (RU-486) and cycloheximide (CHX) did not alter these effects of DEX.
  
  
  CONCLUSIONS
  DEX interferes with the adhesion function of U937 cells through the inhibition of ROCK1 activity.
authors.affiliation: Molecular Biology Center, State Key Laboratory of Trauma, Burn, and Combined Injury, Research Institute of Surgery and Daping Hospital, Third Military Medical University, Chongqing 400042, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.forename: Xing-Yun
authors.initials: XY
authors.lastname: Chen
authors.forename: Ren-Ping
authors.initials: RP
authors.lastname: Xiong
authors.forename: Ya-Lei
authors.initials: YL
authors.lastname: Ning
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Peng
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Nan
authors.initials: N
authors.lastname: Yang
authors.forename: Yuan-Guo
authors.initials: YG
authors.lastname: Zhou
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects*; D003907:Dexamethasone / Q000494:pharmacology*; D015536:Down-Regulation; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D009000:Monocytes / Q000166:cytology / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D020298:U937 Cells; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D003907:Dexamethasone; C515851:ROCK1 protein, human; D054460:rho-Associated Kinases; D013755:Tetradecanoylphorbol Acetate