title: Rapid intracellular growth of gold nanostructures assisted by functionalized graphene oxide and its application for surface-enhanced Raman spectroscopy.
abstract: Hybridization of metal nanoparticles with graphene oxide for high performance surface-enhanced Raman scattering (SERS) has attracted overwhelming attention in recent years. Herein, a one-pot green route for intracellular synthesis of gold nanostructures assisted by poly(vinylpyrrolidone) (PVP)-functionalized graphene oxide (GO) was proposed. The hybrids obtained [GO/PVP/intracellularly grown gold nanoparticles (IGAuNs)] randomly scattered throughout the cell. Compared with the IGAuNs, the growth of GO/PVP/IGAuNs was remarkably accelerated, which could be attributed to the coordination of PVP enriched on GO. GO/PVP/IGAuNs could serve as excellent SERS probes for ultrasensitive detection of cellular components of cancer cells located in the cytoplasm, nucleoplasm, and nucleolus. The random intracellular distribution of GO/PVP/IGAuNs facilitated the effective Raman characterization of cellular components, which was confirmed by the uniform distribution of SERS signals in the Raman image. The SERS signals induced by GO/PVP/IGAuNs could be collected as early as 15 h, which allowed rapid detection of tumor cells. In conclusion, this facile and green strategy for fast intracellular growth of GO/PVP/IGAuNs offered great potential for biomedical applications.
authors.affiliation: MOE Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Liu
authors.forename: Chaofan
authors.initials: C
authors.lastname: Hu
authors.forename: Shaoxin
authors.initials: S
authors.lastname: Li
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Zhouyi
authors.initials: Z
authors.lastname: Guo
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology; D000818:Animals; D005260:Female; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology; D015674:Mammary Neoplasms, Animal / Q000473:pathology; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D049329:Nanostructures / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D013059:Spectrum Analysis, Raman; D014407:Tumor Cells, Cultured
chemical_list: D010087:Oxides; D006046:Gold; D006108:Graphite