title: Immobilized metal ion affinity chromatography: a review on its applications.
abstract: After 35 years of development, immobilized metal ion affinity chromatography (IMAC) has evolved into a popular protein purification technique. This review starts with a discussion of its mechanism and advantages. It continues with its applications which include the purification of histidine-tagged proteins, natural metal-binding proteins, and antibodies. IMAC used in conjunction with mass spectroscopy for phosphoprotein fractionation and proteomics is also covered. Finally, this review addresses the developments, limitations, and considerations of IMAC in the biopharmaceutical industry.
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, China.
authors.forename: Randy Chi Fai
authors.initials: RC
authors.lastname: Cheung
authors.forename: Jack Ho
authors.initials: JH
authors.lastname: Wong
authors.forename: Tzi Bun
authors.initials: TB
authors.lastname: Ng
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008670:Metals / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D008670:Metals; D011506:Proteins