title: Reactive magnetic poly(divinylbenzene-co-glycidyl methacrylate) colloidal particles for specific antigen detection using microcontact printing technique.
abstract: Epoxy-functionalized magnetic poly(divinylbenzene-co-glycidyl methacrylate) colloidal particles (mPDGs) were prepared by co-polymerization of 1,4-divinylbenzene and glycidyl methacrylate monomers. The reaction was conducted by batch emulsion polymerization in the presence of an oil in water magnetic emulsion as a seed. The chemical composition, morphology, iron oxide content, magnetic properties, particle size and colloidal stability of the prepared magnetic polymer particles were characterized using Fourier transform infrared spectroscopy, transmission electron microscopy, thermal gravimetric analysis, vibrating sample magnetometry, dynamic light scattering, and zeta potential determination, respectively. The prepared mPDGs were immobilized on a self-assembled monolayer of 3-aminopropyltriethoxysilane (APTES)/octadecyltrichlorosilane (OTS), which were patterned on glass using microcontact printing technique, forming mPDGs-APTES/OTS reactive surface. This construction (mPDGs-APTES/OTS) was used as a solid support for immunoassay. The immobilized magnetic particles were bioconjugated with monoclonal anti-human IL-10 antibody to provide specific and selective recognition sites for the recombinant human IL-10 protein (antigen). Fluorescence microscopic examination was carried out to follow this immunoassay using fluorescently labeled anti-human IL-10 antibody. The results obtained proved the successful use of mPDGs-APTES/OTS microcontact printed surfaces in an immunoassay, which can be exploited and integrated into microsystems in order to elaborate medical devices (e.g. biosensors) which could provide rapid analysis at high sensitivity with low volumes of analyte.
authors.affiliation: Université de Lyon, F-69622 Lyon, France.
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Eissa
authors.forename: Md
authors.initials: M
authors.lastname: Mahbubor Rahman
authors.forename: Nadia
authors.initials: N
authors.lastname: Zine
authors.forename: Nicole
authors.initials: N
authors.lastname: Jaffrezic
authors.forename: Abdelhamid
authors.initials: A
authors.lastname: Errachid
authors.forename: Hatem
authors.initials: H
authors.lastname: Fessi
authors.forename: Abdelhamid
authors.initials: A
authors.lastname: Elaissari
mesh_terms: D000906:Antibodies / Q000378:metabolism; D000941:Antigens / Q000032:analysis*; D003102:Colloids / Q000737:chemistry*; D004655:Emulsions; D005290:Ferric Compounds / Q000737:chemistry; D005453:Fluorescence; D006801:Humans; D016753:Interleukin-10 / Q000032:analysis*; D060328:Magnetic Phenomena; D046529:Microscopy, Electron, Transmission; D058105:Polymerization; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D011327:Printing / Q000379:methods*; D013194:Staining and Labeling; D055672:Static Electricity; D013818:Thermogravimetry
chemical_list: D000906:Antibodies; D000941:Antigens; D003102:Colloids; D004655:Emulsions; D005290:Ferric Compounds; C508609:IL10 protein, human; D011109:Polymethacrylic Acids; D011145:Polyvinyls; C516630:poly(glycidyl methacrylate-co-divinylbenzene); D016753:Interleukin-10; C000499:ferric oxide