title: [The role of neutrophils in the formation of peritoneal adhesions].
abstract: BACKGROUND
  The use of antibiotics during peritonitis appears to decrease the formation of postoperative intra peritoneal adhesions and reduce their severity. The effect of this antibiotic is still controversial.
  
  
  OBJECTIVE
  To study the relationship between the decrease postoperative adhesions induced by rifamycin, and the number of neutrophils and the number of intraperitoneal bacteria.
  
  
  METHODS
  This is an experimental prospective, randomized singleblind study performed on adult male rats. The product used for the peritoneal lavage was rifamycin s. The animals were randomized into three groups: Group S: intra peritoneal lavage with saline to 9%, R25 Group: intra peritoneal lavage with rifamycin at a dose of 25 mg / kg group and 12.5 R: intra peritoneal lavage with rifamycin at a dose of 12.5 mg / kg. Adhesions score was evaluated according to Zulkhé by the same operator.
  
  
  RESULTS
  The adhesion score was significantly lower between groups S and R12.5 (p = 0.000) and group S and group R25 (P = 0.01). However, the difference was not significant between the two groups R 25 and R12.5 compared to S group (p = 0.655). The number of bacteria between the time of caecal resection (before peritoneal lavage) and the time of death or sacrifice was significantly decreased significantly in the groups R25, comparing the group S (p = 0.003). However, there is no significant difference between groups S and R12, 5 (p = 0.106). The number of neutrophils between the time of cecal resection (before peritoneal lavage) and the time of death or sacrifice decreased significantly in the groups R25 and R12, 5 in comparison to the group S. Between the group R25 and the S group, the difference is significant (p = 0.037) as well between the group R12, 5 and S (p = 0.026). However, there is no significant difference between the two groups R 25 and R12, 5 (p = 0.712).
  
  
  CONCLUSIONS
  The action of rifamycin sodium on neutrophils seems to be independent of its antibacterial action. These findings deserve to be explored at the end to clarify the mechanism of neutropenia by intra peritoneal washing with rifamycin and the relationship between neutropenia and post-operative adhesions.
authors.affiliation: Universite de Sfax, Tunisie.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Jallouli
authors.forename: Ahmed
authors.initials: A
authors.lastname: Hakim
authors.forename: Abir
authors.initials: A
authors.lastname: Znazen
authors.forename: Yosr
authors.initials: Y
authors.lastname: Chaabouni
authors.forename: Zouheir
authors.initials: Z
authors.lastname: Sahnoun
authors.forename: Adnene
authors.initials: A
authors.lastname: Hammami
authors.forename: Khaled
authors.initials: K
authors.lastname: Zeghal
authors.forename: Riadh
authors.initials: R
authors.lastname: Mhiri
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D010532:Peritoneal Diseases / Q000378:metabolism*; D010533:Peritoneal Lavage; D011897:Random Allocation; D051381:Rats; D012294:Rifamycins / Q000008:administration & dosage; D000267:Tissue Adhesions / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D012294:Rifamycins