title: Increase in the production of β-carotene in recombinant Escherichia coli cultured in a chemically defined medium supplemented with amino acids.
abstract: Escherichia coli DH5α strain was selected as the recombinant host, and a chemically defined medium supplemented with amino acids was used instead of a complex medium for the efficient production of β-carotene. In a fed-batch culture using glycerol with a chemically defined medium supplemented with amino acids, the concentration, specific content, and productivity of β-carotene were 2,470 mg/l, 72 mg/g cells, and 77 mg/l h after 32 h, respectively. These values were, respectively, 43, 33, and 26 % higher than those obtained using the complex medium. This is the highest β-carotene production that has been reported among the recombinant cells to date.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, South Korea.
authors.forename: Hyun-Koo
authors.initials: HK
authors.lastname: Nam
authors.forename: Jin-Geun
authors.initials: JG
authors.lastname: Choi
authors.forename: Jae-Hee
authors.initials: JH
authors.lastname: Lee
authors.forename: Seon-Won
authors.initials: SW
authors.lastname: Kim
authors.forename: Deok-Kun
authors.initials: DK
authors.lastname: Oh
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D060847:Metabolic Engineering; D019207:beta Carotene / Q000096:biosynthesis*
chemical_list: D000596:Amino Acids; D003470:Culture Media; D019207:beta Carotene; D005990:Glycerol