title: Contribution of RIZ1 to regulation of proliferation and migration of a liver fluke-related cholangiocarcinoma cell.
abstract: OBJECTIVE
  Retinoblastoma-interacting zinc finger gene (RIZ1) is a tumor suppressor gene which is highly inactivated by promoter hypermethylation in patients with liver fluke-related cholangiocarcinoma (CCA). Epigenetic aberration of this gene might withdraw the ability to restrain tumor cell proliferation and migration. We aimed to define the role of RIZ1 on cell proliferation and migration in CCA cell line.
  
  
  METHODS
  Small interference RNA (siRNA) was used to knock down the expression of RIZ1 in a CCA-derived cell line in which cell proliferation and cell migration were performed.
  
  
  RESULTS
  A predominant nuclear localization of RIZ1 was observed. Reduction of RIZ1 by siRNA augmented cell proliferation and migration.
  
  
  CONCLUSIONS
  The result suggested that RIZ1 might play a role in regulating cell proliferation and migration in CCA. Reduction of RIZ1 expression may aggravate the progression of CCA.
authors.affiliation: Department of Biomedical Sciences, Graduate School, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Prasong
authors.initials: P
authors.lastname: Khaenam
authors.forename: Akiko
authors.initials: A
authors.lastname: Niibori
authors.forename: Seiji
authors.initials: S
authors.lastname: Okada
authors.forename: Patcharee
authors.initials: P
authors.lastname: Jearanaikoon
authors.forename: Norie
authors.initials: N
authors.lastname: Araki
authors.forename: Temduang
authors.initials: T
authors.lastname: Limpaiboon
mesh_terms: D000818:Animals; D001650:Bile Duct Neoplasms / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D001653:Bile Ducts, Intrahepatic / Q000378:metabolism / Q000469:parasitology / Q000473:pathology*; D015153:Blotting, Western; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D018281:Cholangiocarcinoma / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D005210:Fasciola hepatica / Q000502:physiology*; D005211:Fascioliasis / Q000150:complications / Q000378:metabolism / Q000473:pathology; D005455:Fluorescent Antibody Technique; D011495:Histone-Lysine N-Methyltransferase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014945:Wound Healing
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D014157:Transcription Factors; D011495:Histone-Lysine N-Methyltransferase; C093563:PRDM2 protein, human