title: Adult rat hippocampus soluble factors: a novel transplantation model mimicking intracranial microenvironment for tracing the induction and differentiation of adipose-derived stromal cells in vitro.
abstract: Intracranial transplantation of ADSCs induces recovery of CNS diseases, but how they develop in host is poorly understood. The aim of this study is to observe induction and differentiation of ADSCs in the presence of hippocampus soluble factors (HiSF) extracted from the hippocampus of adult Wistar rats to mimic an intracranial microenvironment. To determine the optimal microenvironment, five conditions were tested: 0μg/ml (as control), 50μg/ml, 100μg/ml, 200μg/ml, and 400μg/ml of HiSF. The number of neurospheres was significantly higher in 200μg/ml group than in other groups on the sixth day. Immunofluorescence demonstrated that the neurospheres induced from ADSCs in 200μg/ml group expressed both nestin and CD133, which are more highly expressed in neurospheres than in ADSCs. This result was confirmed by Western blot analysis. Quantitative PCR revealed that the mRNA levels of nestin and CD133 in the neurospheres were 145- and 220-fold higher, respectively, than those in ADSCs. In the presence of 200μg/ml HiSF and 1% FBS, the neurospheres can further differentiate into Schwann-like cells which expressing characteristic markers GFAP, S100 and P75 NGFR. These data indicated that HiSF, mimicking a destination of ADSCs transplanted model in vitro, could effectively induce and differentiate neurospheres, representing a new method to obtain NSCs and Schwann-like cells from ADSCs.
authors.affiliation: The National Key Clinic Specialty, The Neurosurgery Institute of Guangdong Province, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Haitao
authors.initials: H
authors.lastname: Sun
authors.forename: Mouxuan
authors.initials: M
authors.lastname: Du
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Fa
authors.forename: Kun
authors.initials: K
authors.lastname: Qin
authors.forename: Weiwei
authors.initials: W
authors.lastname: Xu
authors.forename: Run
authors.initials: R
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Chen
authors.initials: C
authors.lastname: Yao
authors.forename: Zongyu
authors.initials: Z
authors.lastname: Xiao
authors.forename: Shizhong
authors.initials: S
authors.lastname: Zhang
authors.forename: Yiquan
authors.initials: Y
authors.lastname: Ke
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000071916:AC133 Antigen; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D000831:Animals, Newborn; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D001685:Biological Factors / Q000302:isolation & purification / Q000494:pharmacology; D001921:Brain / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D060833:Cellular Microenvironment; D005109:Extracellular Matrix / Q000737:chemistry; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000737:chemistry; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000166:cytology / Q000637:transplantation
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D001685:Biological Factors; D006023:Glycoproteins; D010455:Peptides; C000606025:Prom1 protein, rat; D012333:RNA, Messenger