title: Salivary markers of oxidative stress in patients with oral premalignant lesions.
abstract: The aetiology of oral premalignant lesions is unknown. Oxidative stress is associated with inflammation and cancerogenesis. The aim of our study was to compare salivary markers of oxidative and carbonyl stress in patients with oral premalignant lesions and age-matched healthy controls. Unstimulated saliva samples were collected from 16 patients with oral premalignant lesions (leukoplakia, lichen planus, erythroplakia) and 16 age-matched healthy controls. Biochemical analysis included measurement of thiobarbituric acid reacting substances (TBARS), advanced oxidation protein products (AOPP), advanced glycation endproducts (AGEs) and total antioxidant capacity (TAC). Salivary RNA was analyzed using real time PCR. Salivary TBARS and AGEs were significantly higher in patients than in controls. No differences were found in AOPP. TAC and expression of superoxide dismutase were lower in patients than in age-matched controls. Other analyzed transcripts (vascular endothelial growth factor, sialotransferase, neuraminidase) did not differ between patients and the control group. Markers of lipoperoxidation and carbonyl stress were increased in patients with oral premalignant lesions. Decreased antioxidant status potentially due to decreased expression of antioxidant enzymes might be responsible for these findings. Our results might point to the aetiology or pathogenesis of oral premalignant lesions as well as to the mechanism of transition to oral carcinoma.
authors.affiliation: Institute of Molecular Biomedicine, Comenius University, Bratislava, Slovakia.
authors.forename: Barbora
authors.initials: B
authors.lastname: Vlková
authors.forename: Peter
authors.initials: P
authors.lastname: Stanko
authors.forename: Gabriel
authors.initials: G
authors.lastname: Minárik
authors.forename: Lubomíra
authors.initials: L
authors.lastname: Tóthová
authors.forename: Tomáš
authors.initials: T
authors.lastname: Szemes
authors.forename: Lenka
authors.initials: L
authors.lastname: Baňasová
authors.forename: Daniela
authors.initials: D
authors.lastname: Novotňáková
authors.forename: Július
authors.initials: J
authors.lastname: Hodosy
authors.forename: Peter
authors.initials: P
authors.lastname: Celec
mesh_terms: D062107:Advanced Oxidation Protein Products / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D017127:Glycation End Products, Advanced / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000378:metabolism; D018384:Oxidative Stress; D011230:Precancerous Conditions / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012463:Saliva / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D062107:Advanced Oxidation Protein Products; D000975:Antioxidants; D014408:Biomarkers, Tumor; D017127:Glycation End Products, Advanced; D017392:Thiobarbituric Acid Reactive Substances; D012313:RNA; D013482:Superoxide Dismutase