title: Isolation and characterization of microsatellite markers for an important tropical tree, Aquilaria malaccensis (Thymelaeaceae).
abstract: OBJECTIVE
  Aggressive collections and trade activities in recent decades have resulted in heavy pressure on the natural stands of Aquilaria malaccensis and concerns over its long-term survival potential. To aid DNA profiling and assessment of its genetic diversity, microsatellite markers were developed for the species.
  
  
  RESULTS
  Seventeen polymorphic microsatellite markers were developed for A. malaccensis using an enrichment protocol. The markers were screened on 24 samples from a natural population. The number of alleles ranged from two to 11, and the observed heterozygosity ranged from 0.042 to 0.957. No significant deviation from Hardy-Weinberg equilibrium was detected after conservative Bonferroni correction.
  
  
  CONCLUSIONS
  This is the first report on the development of microsatellite markers in A. malaccensis. The markers will be used to establish a DNA profiling database and to estimate the genetic diversity and population genetic structure of the species.
authors.affiliation: Genetic Laboratory, Forest Research Institute Malaysia, 52109 Kepong, Selangor, Malaysia.
authors.forename: Lee Hong
authors.initials: LH
authors.lastname: Tnah
authors.forename: Chai Ting
authors.initials: CT
authors.lastname: Lee
authors.forename: Soon Leong
authors.initials: SL
authors.lastname: Lee
authors.forename: Kevin Kit Siong
authors.initials: KK
authors.lastname: Ng
authors.forename: Chin Hong
authors.initials: CH
authors.lastname: Ng
authors.forename: Zakaria
authors.initials: Z
authors.lastname: Nurul-Farhanah
authors.forename: Kah Hoo
authors.initials: KH
authors.lastname: Lau
authors.forename: Lillian Swee Lian
authors.initials: LS
authors.lastname: Chua
mesh_terms: D000483:Alleles; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D029645:Thymelaeaceae / Q000235:genetics*; D014197:Trees / Q000235:genetics*; D014329:Tropical Climate
chemical_list: D017931:DNA Primers; D018744:DNA, Plant