title: Antibacterial activity evaluation of quaternary chitin against Escherichia coli and Staphylococcus aureus.
abstract: Water-soluble quaternary chitin/chitosan derivatives, O-[(2-hydroxy-3-trimethylammonium)propyl chitin (OHT-chitin), N-[(2-hydroxy-3-trimethylammonium)propyl chitosan (NHT-chitosan), and N,N,N-trimethylchitosan (TMC), having identical molecular weight and same anion, were prepared, and their antibacterial activities against Escherichia coli and Staphylococcus aureus were evaluated. Their minimum inhibitory concentration (MIC) values varied from 0.04 mg/mL to 20.48 mg/mL, and the minimum bactericidal concentration (MBC) values varied from 0.16 mg/mL to 40.96 mg/mL against E. coli and S. aureus, respectively. Transmission electron microscopy (TEM) clearly showed that there was serious damage on the bacterial walls, whereas there was some dissimilarity between TMC-treated E. coli and S. aureus.
authors.affiliation: College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310035, PR China. huangjy@mail.zjgsu.edu.cn
authors.forename: Jianying
authors.initials: J
authors.lastname: Huang
authors.forename: Hengjun
authors.initials: H
authors.lastname: Jiang
authors.forename: Miao
authors.initials: M
authors.lastname: Qiu
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Geng
authors.forename: Ronghua
authors.initials: R
authors.lastname: Yang
authors.forename: Jianrong
authors.initials: J
authors.lastname: Li
authors.forename: Chaohua
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D002473:Cell Wall; D002686:Chitin / Q000737:chemistry / Q000494:pharmacology*; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D004926:Escherichia coli / Q000254:growth & development* / Q000648:ultrastructure; D013211:Staphylococcus aureus / Q000254:growth & development* / Q000648:ultrastructure
chemical_list: D000900:Anti-Bacterial Agents; D002686:Chitin; D048271:Chitosan