title: Standardization of a colorimetric method for the determination of fructose using o-cresol: Sulphuric acid reagent.
abstract: Fructose developed a pinkish orange chromogen on treatment with o-cresol: 70% sulphuric acid at 32°C for 15 minutes with a λ max of 500nm. Fructose was 185 times more chromogenic than glucose. Total carbohydrate and fructose values in protein-free filtrate of normal serum samples were in the range, 55.4-86.3 mg/dl and 1.55-3.29 mg/dl, respectively. In diabetes, the observed values were 197-354 mg/dl and 2.91-6.81 mg/dl, respectively.
authors.affiliation: Department of Biochemistry, S. D. M. College of Dental Sciences, 580 009 Sattur, Dharwad.
authors.forename: V
authors.initials: V
authors.lastname: Kumar
authors.forename: T N
authors.initials: TN
authors.lastname: Pattabiraman
keywords: Fructose determination; o-cresol: 70% sulphuric acid; serum values in normals and diabetics