title: Addition of a single N-glycan to street rabies virus glycoprotein enhances virus production.
abstract: Most street rabies virus G proteins have two N-glycosylation sites, i.e. Asn(37) and Asn(319), whereas additional sites are found in fixed (laboratory adapted) viruses. In this study, we performed a pseudotyped virus assay using G-deficient rabies virus and demonstrated that single-N-glycan additions to the G protein of street rabies virus strain 1088, which are found in adapted strains, enhanced virus production in neural and non-neural cell lines, while additions to Asn(194) or Asn(247) enhanced production greatly. Moreover, we found that N-glycan additions at Asn(194) or Asn(247) facilitated the production of cell-associated virus. In contrast, deletion of the sequon at Asn(37) reduced viral production, while a deletion at Asn(319) resulted in extensive loss of production. Furthermore, G proteins lacking an N-glycan at Asn(319) failed to fold into their correct structure and lost their fusion activity, indicating that Asn(319) N-glycosylation is important for the functional expression of street virus G proteins.
authors.affiliation: Research Promotion Institute, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yamada
authors.affiliation: Department of Microbiology, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan.|Research Promotion Institute, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan.
authors.forename: Kazuko
authors.initials: K
authors.lastname: Noguchi
authors.affiliation: Department of Microbiology, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan.
authors.forename: Daichi
authors.initials: D
authors.lastname: Nonaka
authors.affiliation: Department of Microbiology, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan.
authors.forename: Muneshin
authors.initials: M
authors.lastname: Morita
authors.affiliation: Research Promotion Institute, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan.
authors.forename: Aiko
authors.initials: A
authors.lastname: Yasuda
authors.affiliation: Research Promotion Institute, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kawazato
authors.affiliation: Department of Microbiology, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan.|Research Promotion Institute, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Nishizono
mesh_terms: D000956:Antigens, Viral / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006031:Glycosylation; D011134:Polysaccharides / Q000378:metabolism*; D011820:Rabies virus / Q000235:genetics / Q000254:growth & development*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral; D006023:Glycoproteins; D011134:Polysaccharides; D014759:Viral Envelope Proteins; D037521:Virulence Factors; C064951:glycoprotein G, Rabies virus