title: Genomic approaches for interrogating the biochemistry of medicinal plant species.
abstract: Development of next-generation sequencing, coupled with the advancement of computational methods, has allowed researchers to access the transcriptomes of recalcitrant genomes such as those of medicinal plant species. Through the sequencing of even a few cDNA libraries, a broad representation of the transcriptome of any medicinal plant species can be obtained, providing a robust resource for gene discovery and downstream biochemical pathway discovery. When coupled to estimation of expression abundances in specific tissues from a developmental series, biotic stress, abiotic stress, or elicitor challenge, informative coexpression and differential expression estimates on a whole transcriptome level can be obtained to identify candidates for function discovery.
authors.affiliation: Department of Plant Biology, Michigan State University, East Lansing, Michigan, USA.
authors.forename: Elsa
authors.initials: E
authors.lastname: Góngora-Castillo
authors.forename: Greg
authors.initials: G
authors.lastname: Fedewa
authors.forename: Yunsoo
authors.initials: Y
authors.lastname: Yeo
authors.forename: Joe
authors.initials: J
authors.lastname: Chappell
authors.forename: Dean
authors.initials: D
authors.lastname: DellaPenna
authors.forename: C Robin
authors.initials: CR
authors.lastname: Buell
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods; D019295:Computational Biology / Q000379:methods; D030541:Databases, Genetic; D020869:Gene Expression Profiling / Q000379:methods*; D015723:Gene Library; D018745:Genome, Plant; D023281:Genomics / Q000379:methods*; D005838:Genotype; D010946:Plants, Medicinal / Q000737:chemistry* / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D059467:Transcriptome
chemical_list: D018749:RNA, Plant