title: Aberrant expression of collapsin response mediator proteins-1, -2 and -5 in the brain of intrauterine growth restricted rats.
abstract: Intrauterine growth restriction (IUGR) has been associated with increased perinatal morbidity, higher incidence of neurodevelopmental defects and increased risk for adult metabolic syndrome manifestations. Altered protein expression profiles associated with IUGR may be informative on the pathological mechanisms of this condition and might reveal potential markers for postnatal complications. We hypothesized that nutrient manipulation of the pregnant rat might influence the expression of important neurodevelopmental proteins in the resultant IUGR offspring. Therefore, we aimed to determine in newborn rat brain tissue the expression of collapsin response mediator proteins (CRMPs)-1, -2 and -5, commonly referred to as dihydropyrimidinase-related proteins (DPYLs) - playing a role in axon guidance, invasive growth and cell migration - and compare it to the corresponding expression in control rats. Two-dimensional electrophoresis and mass spectrometry, as well as Western blot analysis were employed in brain tissue from 24 IUGR newborn rats and 24 controls. With both methods, CRMP-1, CRMP-2 and CRMP-5 were decreased in the brains of the IUGR group as compared to the control group at the time of delivery. In conclusion, IUGR rat offspring are born with a decreased expression of CRMPs, suggesting that these proteins may be implicated in fetal stress-induced programming.
authors.affiliation: First Department of Pediatrics, Medical School, University of Athens, Aghia Sophia Children's Hospital, Thivon & Papadiamantopoulou str., Athens 11527, Greece. earavidou@aretaieio.uoa.gr
authors.forename: Eftychia
authors.initials: E
authors.lastname: Aravidou
authors.forename: George
authors.initials: G
authors.lastname: Tsangaris
authors.forename: Athina
authors.initials: A
authors.lastname: Samara
authors.forename: Ismene
authors.initials: I
authors.lastname: Dontas
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Botsis
authors.forename: Christos
authors.initials: C
authors.lastname: Aravidis
authors.forename: George P
authors.initials: GP
authors.lastname: Chrousos
authors.forename: Ariadne
authors.initials: A
authors.lastname: Malamitsi-Puchner
mesh_terms: D000818:Animals; D001835:Body Weight; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005317:Fetal Growth Retardation / Q000378:metabolism* / Q000503:physiopathology; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D000098855:Collapsin Response Mediator Protein 1
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D000098855:Collapsin Response Mediator Protein 1; C095114:collapsin response mediator protein-2