title: Invasive candidiasis in Pakistan: clinical characteristics, species distribution and antifungal susceptibility.
abstract: This study reports for the first time, to our knowledge, descriptive epidemiological data for 188 invasive Candida isolates from Pakistan, including species identification and antifungal susceptibility against fluconazole, itraconazole, voriconazole, caspofungin, micafungin, anidulafungin and amphotericin. Risk factors for invasive candidiasis (IC) were determined for 96 patients from Karachi, Pakistan. In adults and neonates, Candida tropicalis (38 and 36 %, respectively) was the most common species, followed in adults by Candida parapsilosis (17.8 %), Candida glabrata (15.9 %) and Candida albicans (12.3 %). C. albicans (21 %) was the second most common in neonates. In children, C. albicans (31.9 %), C. tropicalis (26.4 %) and C. parapsilosis (19.4 %) were the most common. C. albicans IC was significantly associated with paediatric age [crude odds ratio (COR) 3.46, 95 % confidence interval (CI) 1.63-7.32]. Rare species made up 17.5 % of the total isolates studied. Resistance to fluconazole was seen in C. glabrata (15 .0%) and Candida krusei (100 .0%). Only one isolate (C. glabrata) was resistant to all three echinocandins. Low MICs of fluconazole for 98 % (184/188) of isolates tested support its continued use as an empiric therapy for IC. Non-C. albicans IC was associated with the use of β-lactam inhibitor combinations (COR 3.16, 95 % CI 1.05-9.57). Use of healthcare devices was documented in 85.4 % of IC patients, whilst 75 .0% had been admitted to special care units. Surprisingly, 66.7 % of patients with IC were not obviously immunosuppressed. The high frequency of modifiable risk factors in this population indicates that candidaemia can be reduced with stringent antibiotic and infection control measures. These data will be useful for empiric selection of antifungals in Karachi, and contribute to global assessments of antifungal resistance.
authors.affiliation: Department of Pathology Microbiology, Aga Khan Hospital Karachi, Pakistan.
authors.forename: J Q
authors.initials: JQ
authors.lastname: Farooqi
authors.affiliation: Department of Pathology Microbiology, Aga Khan Hospital Karachi, Pakistan.
authors.forename: K
authors.initials: K
authors.lastname: Jabeen
authors.affiliation: Department of Pathology Microbiology, Aga Khan Hospital Karachi, Pakistan.
authors.forename: N
authors.initials: N
authors.lastname: Saeed
authors.affiliation: Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: N
authors.initials: N
authors.lastname: Iqbal
authors.affiliation: Department of Pathology Microbiology, Aga Khan Hospital Karachi, Pakistan.
authors.forename: B
authors.initials: B
authors.lastname: Malik
authors.affiliation: Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: S R
authors.initials: SR
authors.lastname: Lockhart
authors.affiliation: Department of Pathology Microbiology, Aga Khan Hospital Karachi, Pakistan.
authors.forename: A
authors.initials: A
authors.lastname: Zafar
authors.affiliation: Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: M E
authors.initials: ME
authors.lastname: Brandt
authors.affiliation: Department of Pathology Microbiology, Aga Khan Hospital Karachi, Pakistan.
authors.forename: R
authors.initials: R
authors.lastname: Hasan
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D058365:Candidiasis, Invasive / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology*; D002648:Child; D002675:Child, Preschool; D025141:Drug Resistance, Fungal; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D010154:Pakistan / Q000453:epidemiology; D055815:Young Adult
chemical_list: D000935:Antifungal Agents