title: Association between ADIPOQ SNPs with plasma adiponectin and glucose homeostasis and adiposity phenotypes in the IRAS Family Study.
abstract: BACKGROUND
  Adiponectin is an adipocytokine associated with a variety of metabolic traits. These associations in human studies, in conjunction with functional studies in model systems, have implicated adiponectin in multiple metabolic processes.
  
  
  OBJECTIVE
  We hypothesize that genetic variants associated with plasma adiponectin would also be associated with glucose homeostasis and adiposity phenotypes.
  
  
  METHODS
  The Insulin Resistance Atherosclerosis Family Study was designed to identify the genetic and environmental basis of insulin resistance and adiposity in the Hispanic- (n=1,424) and African-American (n=604) population.
  
  
  METHODS
  High quality metabolic phenotypes, e.g. insulin sensitivity (S(I)), acute insulin response (AIR), disposition index (DI), fasting glucose, body mass index (BMI), visceral adipose tissue (VAT), subcutaneous adipose tissue (SAT), and waist circumference, were explored.
  
  
  RESULTS
  Based on association analysis of more than 40 genetic polymorphisms in the adiponectin gene (ADIPOQ), we found no consistent association of ADIPOQ variants with plasma adiponectin levels and adiposity phenotypes. However, there were two promoter variants, rs17300539 and rs822387, associated with plasma adiponectin levels (P=0.0079 and 0.021, respectively) in the Hispanic-American cohort that were also associated with S(I) (P=0.0067 and 0.013, respectively). In contrast, there was only a single promoter SNP, rs17300539, associated with plasma adiponectin levels (P=0.0018) and fasting glucose (P=0.042) in the African-American cohort. Strikingly, high impact coding variants did not show evidence of association.
  
  
  CONCLUSIONS
  The lack of consistent patterns of association between variants, adiponectin levels, glucose homeostasis, and adiposity phenotypes suggests a reassessment of the influence of adiponectin in these pathways.
authors.affiliation: Department of Biochemistry, Wake Forest School of Medicine, Winston-Salem, NC 27157, USA.
authors.forename: S Sandy
authors.initials: SS
authors.lastname: An
authors.forename: Anthony J G
authors.initials: AJ
authors.lastname: Hanley
authors.forename: Julie T
authors.initials: JT
authors.lastname: Ziegler
authors.forename: W Mark
authors.initials: WM
authors.lastname: Brown
authors.forename: Steven M
authors.initials: SM
authors.lastname: Haffner
authors.forename: Jill M
authors.initials: JM
authors.lastname: Norris
authors.forename: Jerome I
authors.initials: JI
authors.lastname: Rotter
authors.forename: Xiuqing
authors.initials: X
authors.lastname: Guo
authors.forename: Y-D Ida
authors.initials: YD
authors.lastname: Chen
authors.forename: Lynne E
authors.initials: LE
authors.lastname: Wagenknecht
authors.forename: Carl D
authors.initials: CD
authors.lastname: Langefeld
authors.forename: Donald W
authors.initials: DW
authors.lastname: Bowden
authors.forename: Nicholette D
authors.initials: ND
authors.lastname: Palmer
mesh_terms: D052242:Adiponectin / Q000097:blood* / Q000235:genetics*; D050154:Adiposity / Q000235:genetics*; D000328:Adult; D001741:Black or African American / Q000235:genetics; D000483:Alleles; D005260:Female; D056726:Genetic Association Studies; D005947:Glucose / Q000378:metabolism*; D006630:Hispanic or Latino / Q000235:genetics; D006706:Homeostasis / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin; D005947:Glucose