title: UDP-N-acetylglucosamine transporter and UDP-galactose transporter form heterologous complexes in the Golgi membrane.
abstract: UDP-galactose transporter (UGT; SLC35A2) and UDP-N-acetylglucosamine transporter (NGT; SLC35A3) are evolutionarily related. We hypothesize that their role in glycosylation may be coupled through heterologous complex formation. Coimmunoprecipitation analysis and FLIM-FRET measurements performed on living cells showed that NGT and UGT form complexes when overexpressed in MDCK-RCA(r) cells. We also postulate that the interaction of NGT and UGT may explain the dual localization of UGT2. For the first time we demonstrated in vivo homodimerization of the NGT nucleotide sugar transporter. In conclusion, we suggest that NGT and UGT function in glycosylation is combined via their mutual interaction.
authors.affiliation: Laboratory of Biochemistry, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Maszczak-Seneczko
authors.forename: Paulina
authors.initials: P
authors.lastname: Sosicka
authors.forename: Michał
authors.initials: M
authors.lastname: Majkowski
authors.forename: Teresa
authors.initials: T
authors.lastname: Olczak
authors.forename: Mariusz
authors.initials: M
authors.lastname: Olczak
mesh_terms: D000818:Animals; D002460:Cell Line; D004285:Dogs; D006056:Golgi Apparatus / Q000378:metabolism*; D047468:Immunoprecipitation; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D000099237:UDP-Galactose Translocators
chemical_list: D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D000099237:UDP-Galactose Translocators; C121945:UDP-N-acetylglucosamine transporter