title: RAPD fingerprint construction and genetic similarity of Mesona  chinensis (Lamiaceae) in China.
abstract: Mesona chinensis is an economically important agricultural crop,  primarily cultivated for making grass jelly. It was originally discovered in  South China. We examined 18 cultivars, including cultivars from Guangdong,  Fujian, and Guangxi, China, Taiwan, and Indonesia, and a hybrid (a cross  between cultivars from Indonesia and Guangdong), based on RAPD markers. The  genetic similarity coefficient was calculated by NTSYS 2.10 and the clustering  analysis was made by UPGMA. PCR amplification with 10 primers produced 163  bands; 94% of the amplified loci were polymorphic. The primers S208, S206, and  S253 could completely distinguish all 19 samples by constructing a DNA  fingerprint. Cluster analysis divided the 19 cultivars into five groups, with  an overall genetic similarity coefficient of 0.68. Correlations were found  among regional distributions, parental sources, and RAPD markers, demonstrating  the rich genetic diversity of these 19 cultivars of M. chinensis. This  study provides useful information for the classification, identification, and  breeding of M. chinensis.
authors.affiliation: College of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
authors.forename: G F
authors.initials: GF
authors.lastname: Zhang
authors.forename: J M
authors.initials: JM
authors.lastname: Guan
authors.forename: X P
authors.initials: XP
authors.lastname: Lai
authors.forename: J
authors.initials: J
authors.lastname: Lin
authors.forename: J M
authors.initials: JM
authors.lastname: Liu
authors.forename: H H
authors.initials: HH
authors.lastname: Xu
mesh_terms: D002681:China; D016000:Cluster Analysis; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics; D005838:Genotype; D019686:Lamiaceae / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*
chemical_list: D017931:DNA Primers; D018744:DNA, Plant