title: Poly A RNA status and expression of milli (m) and micro (μ) calpains in skeletal muscle of duchenne muscular dystrophy patients.
abstract: Poly A RNA status and itsin vitro translation in a rabbit reticulolysate cell free system, were assessed in the skeletal muscle of young boys with Duchenne muscular dystrophy. Compared to normals there is a significant 48% increase in poly A RNA content of dystrophic muscle and its translatability was increased by 56% based on(35)S methionine incorporation into total protein systhesised. Immunoprecipitation of the translated products with monospecific antibodies showed that there is a 2.6 fold and a 2 fold increase in m and μ calpains respectively. This underlines the importance of both synthetic and degenerative activities in the early pathology in DMD muscle.
authors.affiliation: Department of Molecular Biology & Biochemistry, Institute of Genetics & Hospital for Genetic Diseases, Begumpet, 500 016 Hyderahad, India.
authors.forename: Harleen
authors.initials: H
authors.lastname: Mangat
authors.forename: Tajamul
authors.initials: T
authors.lastname: Hussain
authors.forename: C
authors.initials: C
authors.lastname: Sundaram
authors.forename: Mp Js
authors.initials: MJ
authors.lastname: Anandaraj
keywords: Calpain; DMD; Protein synthesis; Skeletal muscle; in vitro translation; poly A RNA