title: In vitro and in vivo protective effects of fermented preparations of dietary herbs against lipopolysaccharide insult.
abstract: Lipopolysaccharide (LPS) is known to produce endotoxic shock by triggering systemic inflammatory responses. Here, we evaluated the protective effects of three fermented/re-fermented herbs, Rhizoma Atractylodis Macrocephalae, Massa Medicata Fermentata, and Dolichoris Semen, in an LPS-mediated inflammatory insult, either individually in vitro using RAW264.7 cells or in combination in in vivo using rats. In general, each of the fermented herbs showed appreciable in vitro anti-inflammatory activity, although the degree of this activity varied with the herb used. Moreover, a mixture of fermented herbal extracts in combination with probiotics significantly attenuated the blood endotoxin and CRP levels, as well as the gut permeability, and significantly augmented the intestinal Lactobacillus spp. colonisation in LPS-treated rats. However, these effects were not observed following the administration of the corresponding mixture of unfermented herbal extracts. Thus, our results highlight the beneficial impacts of the use of fermented herb products with probiotics to combat LPS-mediated inflammatory insults.
authors.affiliation: Institute of Medical Research, College of Medicine, Dongguk University Seoul, Republic of Korea.
authors.forename: Shambhunath
authors.initials: S
authors.lastname: Bose
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Song
authors.forename: Jong-Kyoung
authors.initials: JK
authors.lastname: Nam
authors.forename: Myeong-Jong
authors.initials: MJ
authors.lastname: Lee
authors.forename: Hojun
authors.initials: H
authors.lastname: Kim
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000378:metabolism; D001407:Bacillus / Q000378:metabolism; D002460:Cell Line; D005285:Fermentation; D006801:Humans; D007249:Inflammation / Q000188:drug therapy* / Q000276:immunology; D007934:Leuconostoc / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology*; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage* / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry* / Q000378:metabolism / Q000382:microbiology; D019936:Probiotics / Q000008:administration & dosage*; D020011:Protective Agents / Q000008:administration & dosage* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D010936:Plant Extracts; D020011:Protective Agents