title: Influence of trace elements on stabilization of aqueous solutions of ascorbic acid.
abstract: Together with vitamin C, zinc, selenium, manganese, and magnesium play a vital role in the preservation of organs scheduled for transplantation. In the present study, it is shown that addition of 1 mg/l of these elements influences the stability of 0.3 mM ascorbic acid solutions. The solution's stability was estimated using an accelerated stability test. The concentration of vitamin C was measured using a validated spectrophotometric method, which uses the reduction of 2,6-dichlorophenoloindophenol by ascorbic acid. Elevated temperatures, the factor accelerating substances' decomposition reaction rate, were used in the tests. The research was conducted at two temperatures at intervals of 10 °C: 80 ± 0.1 and 90 ± 0.1 °C. It was stated that the studied substances' decomposition occurred in accordance with the equation for first-order reactions. The function of the logarithmic concentration (log%C) over time was revealed to be rectilinear. This dependence was used to determine the kinetics of decomposition reaction rate parameters. The stabilization of vitamin C solutions was measured as the time in which 10 % of the substance decomposed at 20 and 0 °C. Addition of Se(IV) or Mg(II) ions significantly increase the stability of ascorbic acid solution (∼34 and ∼16 %, respectively), but Zn(II) causes a significant decrease in stability by ∼23 %. Addition of Mn(II) has no significant influence on vitamin C stability.
authors.affiliation: Biochefa Pharmaceutical Research and Production Plant, Sosnowiec, Poland. gix@o2.pl
authors.forename: Barbara
authors.initials: B
authors.lastname: Dolińska
authors.forename: Aneta
authors.initials: A
authors.lastname: Ostróżka-Cieślik
authors.forename: Artur
authors.initials: A
authors.lastname: Caban
authors.forename: Klimas
authors.initials: K
authors.lastname: Rimantas
authors.forename: Lucyna
authors.initials: L
authors.lastname: Leszczyńska
authors.forename: Florian
authors.initials: F
authors.lastname: Ryszka
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000737:chemistry*; D001205:Ascorbic Acid / Q000032:analysis / Q000737:chemistry*; D004355:Drug Stability; D005650:Fumarates / Q000737:chemistry; D006358:Hot Temperature; D007700:Kinetics; D008274:Magnesium / Q000737:chemistry*; D008345:Manganese / Q000737:chemistry; D017895:Manganese Compounds / Q000737:chemistry; D019188:Organ Preservation Solutions / Q000737:chemistry; D009994:Osmolar Concentration; D010084:Oxidation-Reduction; D011310:Preservatives, Pharmaceutical / Q000737:chemistry; D012643:Selenium / Q000737:chemistry*; D018038:Sodium Selenite / Q000737:chemistry; D013431:Sulfates / Q000737:chemistry; D014131:Trace Elements / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry; D019345:Zinc Acetate / Q000737:chemistry
chemical_list: D000975:Antioxidants; D005650:Fumarates; D017895:Manganese Compounds; D019188:Organ Preservation Solutions; D011310:Preservatives, Pharmaceutical; D013431:Sulfates; D014131:Trace Elements; D008345:Manganese; C032005:fumaric acid; D019345:Zinc Acetate; D012643:Selenium; D018038:Sodium Selenite; D008274:Magnesium; D015032:Zinc; D001205:Ascorbic Acid; C039798:manganese sulfate