title: Preparation, characterization and efficacy evaluation of synthetic biocompatible polymers linking natural antioxidants.
abstract: The purpose of this work was the synthesis, characterization and efficacy evaluation of new biocompatible antioxidant polymers linking trans-ferulic acid or  a-lipoic acid. In particular, ferulic or lipoic acid were introduced in the preformed polymeric backbone. The new antioxidant biopolymers were characterized by Fourier transform infrared spectroscopy and gel permeation chromatography. The degree of functionalization (moles of antioxidant per gram of polymer) was determined by the  Gaur-Gupta method for free amino group determination and by the Folin method for the phenolic groups. Their ability to inhibit lipid peroxidation were estimated in rat liver microsomal membranes induced in vitro by tert-BOOH (tert-butyl hydroperoxide), as a source of free radicals. The DPPH (1,1-diphenyl-2-picrylhydrazyl) radical-scavenging effect was also evaluated. The obtained systems, with different solubility, showed strong antioxidant and antiradical activities, suggesting potential use as packaging materials for foods, cosmetics, pharmaceuticals and personal care products. Moreover, the cytotoxicity of the synthesized polymers was also evaluated on Caco-2 cell cultures in order to verify their biocompatibility when exposed to an absorptive epithelial cell line.
authors.affiliation: Department of Pharmaceutical Sciences, University of Calabria, Arcavacata di Rende 87036, Cosenza, Italy.
authors.forename: Sonia
authors.initials: S
authors.lastname: Trombino
authors.forename: Roberta
authors.initials: R
authors.lastname: Cassano
authors.forename: Teresa
authors.initials: T
authors.lastname: Ferrarelli
authors.forename: Sonia
authors.initials: S
authors.lastname: Leta
authors.forename: Francesco
authors.initials: F
authors.lastname: Puoci
authors.forename: Nevio
authors.initials: N
authors.lastname: Picci
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D000180:Acrylic Resins / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects; D003373:Coumaric Acids / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D005609:Free Radicals / Q000737:chemistry; D006801:Humans; D020128:Inhibitory Concentration 50; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D008689:Methacrylates / Q000737:chemistry; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D010851:Picrates / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D017550:Spectroscopy, Fourier Transform Infrared; D008063:Thioctic Acid / Q000737:chemistry*; D000099166:Polyacrylamides
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D001672:Biocompatible Materials; D001713:Biphenyl Compounds; D003373:Coumaric Acids; D016166:Free Radical Scavengers; D005609:Free Radicals; D008315:Malondialdehyde; D008689:Methacrylates; D010851:Picrates; D008063:Thioctic Acid; D000099166:Polyacrylamides; C072703:2-aminoethylmethacrylate; C099046:N,N-dimethylacrylamide; C004999:ferulic acid; C004931:1,1-diphenyl-2-picrylhydrazyl