title: Poly(hydroxyethyl methacrylate) based magnetic nanoparticles for lysozyme purification from chicken egg white.
abstract: The purpose of this article is to synthesize poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-tryptophan) [mPHEMATrp] magnetic nanoparticles for lysozyme purification from chicken egg white. mPHEMATrp nanoparticles (38 nm in diameter) were synthesized by surfactant-free emulsion polymerization. Specific surface area of the mPHEMATrp nanoparticles was calculated to be 896 m2/g. Elemental analysis of mPHEMATrp nanoparticles was estimated in the range of 5.7-48.3 µmol MATrp/g. The maximum lysozyme adsorption capacity of the mPHEMATrp nanoparticles was 376.1 mg/g. The mPHEMATrp nanoparticles could be used five times without decreasing the lysozyme adsorption capacity significantly. The results indicate that the mPHEMATrp nanoparticles promise high selectivity for lysozyme purification.
authors.affiliation: Department of Chemistry, Biochemistry Division, Hacettepe University, Ankara, Turkey.
authors.forename: Kazım
authors.initials: K
authors.lastname: Köse
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D000327:Adsorption; D000818:Animals; D002645:Chickens; D004529:Egg White / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D009113:Muramidase / Q000302:isolation & purification*; D011102:Polyhydroxyethyl Methacrylate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism; D012680:Sensitivity and Specificity; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism
chemical_list: D058185:Magnetite Nanoparticles; C542088:poly(hydroxyeethyl methacrylate-N-methacryloyl-tyrosine methyl ester); D011102:Polyhydroxyethyl Methacrylate; D014443:Tyrosine; C421864:hen egg lysozyme; D009113:Muramidase