title: Rapid Detection of Escherichia coli O157: H7 by Fluorescent Amplification-Based Specific Hybridization (FLASH) PCR.
abstract: BACKGROUND
  Escherichia coli O157:H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans.
  
  
  METHODS
  We developed fluorescent amplification-based specific hybridization (FLASH-PCR) assay to detect the Stx-encoding gene Stx-1 of E. coli O157:H7.
  
  
  RESULTS
  PCR product of 336 bp was successfully amplified in a FLASH-PCR.
  
  
  CONCLUSIONS
  As rapid detection and confirmation of the presence of E. coli O157:H7 are of importance for the medical, food, and water industries, FLASH-PCR is one of selective methods for detection of E. coli O157:H7.
authors.affiliation: Department of Biology, Science School, Ferdowsi University, Mashhad, Iran.
authors.forename: F
authors.initials: F
authors.lastname: Khatami
authors.forename: M
authors.initials: M
authors.lastname: Heidari
authors.forename: M
authors.initials: M
authors.lastname: Khatami
keywords: Detection; E.coli O157:H7; FLASH-PCR; Stx-1 gene