title: Metagenomics: Future of microbial gene mining.
abstract: Modern biotechnology has a steadily increasing demand for novel genes for application in various industrial processes and development of genetically modified organisms. Identification, isolation and cloning for novel genes at a reasonable pace is the main driving force behind the development of unprecedented experimental approaches. Metagenomics is one such novel approach for engendering novel genes. Metagenomics of complex microbial communities (both cultivable and uncultivable) is a rich source of novel genes for biotechnological purposes. The contributions made by metagenomics to the already existing repository of prokaryotic genes is quite impressive but nevertheless, this technique is still in its infancy. In the present review we have drawn comparison between routine cloning techniques and metagenomic approach for harvesting novel microbial genes and described various methods to reach down to the specific genes in the metagenome. Accomplishments made thus far, limitations and future prospects of this resourceful technique are discussed.
authors.affiliation: Department of Biotechnology, University of Jammu, Jammu, 180 006 India.
authors.forename: J
authors.initials: J
authors.lastname: Vakhlu
authors.forename: Avneet Kour
authors.initials: AK
authors.lastname: Sudan
authors.forename: B N
authors.initials: BN
authors.lastname: Johri
keywords: Metagenomics; Microbes; Novel genes; Uncultivable