title: Effectiveness of adjunctive subgingival administration of amino acids and sodium hyaluronate gel on clinical and immunological parameters in the treatment of chronic periodontitis.
abstract: OBJECTIVE
  The aim of this clinical trial was to compare clinical and biochemical healing outcomes following ultrasonic mechanical instrumentation versus ultrasonic mechanical instrumentation associated with topical subgingival application of amino acids and sodium hyaluronate gel.
  
  
  METHODS
  Eleven systemically healthy subjects with moderate-severe chronic periodontitis, who had four sites with pocket probing depth and clinical attachment level greater than or equal to 5 mm were randomly assigned to two different types of treatment: two pockets were treated with ultrasonic debridement (Control Group) and two pockets with ultrasonic mechanical instrumentation associated with 0,5 ml of amino acids and sodium hyaluronate gel (Test Group). Probing depth, clinical attachment level, plaque index and bleeding on probing were recorded at baseline, 45 and 90 days. Levels of calprotectin and myeloperoxidase activity in gingival crevicular fluid were assessed at baseline and on day 7 and 45.
  
  
  RESULTS
  Statistical significance was found between baseline and day 45 in relation to probing depth reduction and bleeding on probing between groups for both of the tested treatments. Significant reductions in μg/sample of calprotectin and myeloperoxidase were found after 1-week and an increase at 45 days in both groups. There were no statistically significant differences between other variables evaluated in this study.
  
  
  CONCLUSIONS
  These data suggest that subgingival application of hyaluronic acid following ultrasonic mechanical instrumentation is beneficial for improving periodontal parameters.
authors.affiliation: Department of Clinical Medical Sciences, Surgical and Health University of Trieste, Trieste, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Bevilacqua
authors.forename: Jessica
authors.initials: J
authors.lastname: Eriani
authors.forename: Ilde
authors.initials: I
authors.lastname: Serroni
authors.forename: Giuliana
authors.initials: G
authors.lastname: Liani
authors.forename: Violetta
authors.initials: V
authors.lastname: Borelli
authors.forename: Gaetano
authors.initials: G
authors.lastname: Castronovo
authors.forename: Roberto
authors.initials: R
authors.lastname: Di Lenarda
keywords: calprotectin; hyaluronic acid; myeloperoxidase; periodontitis