title: Discovery of novel integrin ligands from combinatorial libraries using a multiplex "beads on a bead" approach.
abstract: The development of screening approaches to identify novel affinity ligands has paved the way for a new generation of molecular targeted nanomedicines. Conventional methods typically bias the display of the target protein to ligands during the screening process. We have developed an unbiased multiplex "beads on a bead" strategy to isolate, characterize, and validate high affinity ligands from OBOC libraries. Novel non-RGD peptides that target α(v)β(3) integrin were discovered that do not affect cancer or endothelial cell biology. The peptides identified here represent novel integrin-targeted agents that can be used to develop targeted nanomedicines without the risk of increased tumor invasion and metastasis.
authors.affiliation: Department of Medical Biophysics, MSB-415A, The University of Western Ontario, London, Ontario, N6A 5C1 Canada.
authors.forename: Choi-Fong
authors.initials: CF
authors.lastname: Cho
authors.forename: Giulio A
authors.initials: GA
authors.lastname: Amadei
authors.forename: Daniel
authors.initials: D
authors.lastname: Breadner
authors.forename: Leonard G
authors.initials: LG
authors.lastname: Luyt
authors.forename: John D
authors.initials: JD
authors.lastname: Lewis
mesh_terms: D001710:Biotin / Q000737:chemistry; D019921:Biotinylation; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D003094:Collagen / Q000737:chemistry; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D003584:Cytological Techniques; D004338:Drug Combinations; D042783:Endothelial Cells / Q000166:cytology; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000378:metabolism; D007797:Laminin / Q000737:chemistry; D008024:Ligands; D008280:Magnetics; D008856:Microscopy, Fluorescence / Q000379:methods; D009369:Neoplasms / Q000378:metabolism; D009842:Oligopeptides / Q000737:chemistry; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry; D011509:Proteoglycans / Q000737:chemistry
chemical_list: D004338:Drug Combinations; D039302:Integrin alphaVbeta3; D007797:Laminin; D008024:Ligands; D009842:Oligopeptides; D019151:Peptide Library; D010455:Peptides; D011509:Proteoglycans; C059485:matrigel; D001710:Biotin; C047981:arginyl-glycyl-aspartic acid; D003094:Collagen