title: Assessment of change in biofilm architecture by nutrient concentration using a multichannel microdevice flow system.
abstract: A new multichannel microdevice flow system with stainless steel flow chamber was used for architecture visualization, development monitoring and structural quantification of GFP-labeled Pseudomonas aeruginosa PAO1 live biofilms. Direct in situ investigations using confocal laser scanning microscopy (CLSM) at 72 h revealed structural pattern differences as a result of nutrient concentration gradients. When grown in LB medium, round, dispersed cellular aggregates were formed whereas in 1/3-diluted LB medium, biofilms were mostly flat and compact. However, COMSTAT analyses showed no considerable differences in biomass and thickness between the two LB concentrations. Characterization of time-dependent development of biofilms grown in 1/3-diluted LB medium showed full maturation of colonies by 120 h reaching maximum biomass at 17.1 μm(3)/μm(2) and average thickness at 44.4 μm. Consequent thinning and formation of openings through interior in colonies occurred by 168 h. These results suggest that the new system tested allowed a fast and thick biofilm development on the surface of the stainless steel flow chamber. These findings may provide better estimates of biofilm activity and systematic evaluation of the effects of different parameters on biofilm morphology and development in industrial and biomedical systems.
authors.affiliation: Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama, Japan.
authors.forename: Zoe
authors.initials: Z
authors.lastname: Sanchez
authors.forename: Akio
authors.initials: A
authors.lastname: Tani
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Suzuki
authors.forename: Reiko
authors.initials: R
authors.lastname: Kariyama
authors.forename: Hiromi
authors.initials: H
authors.lastname: Kumon
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Kimbara
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D018533:Biomass; D003470:Culture Media; D049452:Green Fluorescent Proteins / Q000032:analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D018613:Microscopy, Confocal; D011550:Pseudomonas aeruginosa / Q000502:physiology
chemical_list: D003470:Culture Media; D049452:Green Fluorescent Proteins