title: Inhibiting wear particles-induced osteolysis with naringin.
abstract: OBJECTIVE
  The purpose of this study was to determine the effects of naringin on osteoclastogenesis and osteolysis both in vitro and in vivo.
  
  
  METHODS
  In this research osteoclasts were generated from mouse bone marrow monocytes with the receptor activator of NF-КB ligand and the macrophage colony stimulating factor. Naringin, at a concentration of 1, 10, 50, and 100 μg/mL, was respectively added to the medium. Seven days later, the osteoclasts were determined through tartrate-resistant acid phosphatase (TRAP) staining. Mature osteoclasts were isolated from newborn rabbits and cultured for three days on bone slices. Naringin at a concentration of 1, 10, 50, and 100 μg/mL was respectively added to the medium. The resorption bone slices were quantified, and the area was calculated after toluidine blue and Mayer-hematoxylin staining. Polymethyl methacrylate (PMMA) particles were implanted on the calvariae of C57BL/J6 mice. Naringin, at a dose of 50 μg/kg and 100 μg/kg, was respectively given intraperitoneally for seven. Seven days later, the calvariae were removed and processed for pathological analysis.
  
  
  RESULTS
  The result indicated that naringin treatment effectively inhibited in vitro osteoclastogenesis and inhibited mature osteoclasts. In vivo data indicated that naringin strongly inhibited PMMA-induced osteolysis.
  
  
  CONCLUSIONS
  Naringin can effectively inhibit osteoclastogenesis and suppress wear particles-induced osteolysis and might be useful in the treatment or prevention of wear particles-induced osteolysis and aseptic loosening for its effect on osteoclast generation and function.
authors.affiliation: Department of Orthopaedics, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Yu
authors.forename: Xingwei
authors.initials: X
authors.lastname: Zhao
authors.forename: Tianyi
authors.initials: T
authors.lastname: Wu
authors.forename: Zubin
authors.initials: Z
authors.lastname: Zhou
authors.forename: Youshui
authors.initials: Y
authors.lastname: Gao
authors.forename: Xinfu
authors.initials: X
authors.lastname: Wang
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Zhang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001862:Bone Resorption; D002478:Cells, Cultured; D004195:Disease Models, Animal; D044950:Flavanones / Q000494:pharmacology*; D066298:In Vitro Techniques; D051379:Mice; D010010:Osteoclasts / Q000187:drug effects*; D010014:Osteolysis / Q000139:chemically induced* / Q000517:prevention & control*; D019904:Polymethyl Methacrylate / Q000633:toxicity*; D011817:Rabbits; D012886:Skull / Q000187:drug effects*; D013194:Staining and Labeling
chemical_list: D044950:Flavanones; D019904:Polymethyl Methacrylate; C005274:naringin