title: Proteomic analysis of a decellularized human vocal fold mucosa scaffold using 2D electrophoresis and high-resolution mass spectrometry.
abstract: Natural biologic scaffolds for tissue engineering are commonly generated by decellularization of tissues and organs. Despite some preclinical and clinical success, in vivo scaffold remodeling and functional outcomes remain variable, presumably due to the influence of unidentified bioactive molecules on the scaffold-host interaction. Here, we used 2D electrophoresis and high-resolution mass spectrometry-based proteomic analyses to evaluate decellularization effectiveness and identify potentially bioactive protein remnants in a human vocal fold mucosa model. We noted proteome, phosphoproteome and O-glycoproteome depletion post-decellularization, and identified >200 unique protein species within the decellularized scaffold. Gene ontology-based enrichment analysis revealed a dominant set of functionally-related ontology terms associated with extracellular matrix assembly, organization, morphology and patterning, consistent with preservation of a tissue-specific niche for later cell seeding and infiltration. We further identified a subset of ontology terms associated with bioactive (some of which are antigenic) cellular proteins, despite histological and immunohistochemical data indicating complete decellularization. These findings demonstrate the value of mass spectrometry-based proteomics in identifying agents potentially responsible for variation in host response to engineered tissues derived from decellularized scaffolds. This work has implications for the manufacturing of biologic scaffolds from any tissue or organ, as well as for prediction and monitoring of the scaffold-host interaction in vivo.
authors.affiliation: Division of Otolaryngology, Department of Surgery, University of Wisconsin School of Medicine and Public Health, Madison, WI 53792, USA. welham@surgery.wisc.edu
authors.forename: Nathan V
authors.initials: NV
authors.lastname: Welham
authors.forename: Zhen
authors.initials: Z
authors.lastname: Chang
authors.forename: Lloyd M
authors.initials: LM
authors.lastname: Smith
authors.forename: Brian L
authors.initials: BL
authors.lastname: Frey
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D005109:Extracellular Matrix / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry; D020543:Proteome / Q000032:analysis* / Q000302:isolation & purification; D040901:Proteomics; D054457:Tissue Scaffolds / Q000737:chemistry; D014827:Vocal Cords / Q000737:chemistry* / Q000166:cytology
chemical_list: D020543:Proteome