title: Association between the CD14 gene C-159T polymorphism and serum soluble CD14 with pulmonary tuberculosis.
abstract: BACKGROUND
  Functional C-159T polymorphism in the promoter region of the CD14 lipopolysaccharide receptor has been reported to be associated with the development of tuberculosis (TB).
  
  
  OBJECTIVE
  To assess the association of CD14 C-159T polymorphism and serum soluble CD14 (sCD14)  levels with pulmonary tuberculosis (TB) in an Iranian population living in a TB-endemic area.
  
  
  METHODS
  A case-control study was performed prospectively on 120 newly diagnosed pulmonary TB patients and 131 healthy subjects. C-159T polymorphism was performed using amplification refractory  mutation system polymerase chain reaction (ARMS-PCR). Concentrations of sCD14 were measured in serum samples using enzyme-linked immunosorbent assay.
  
  
  RESULTS
  The genotype frequencies of C-159T polymorphism differed significantly between TB patients and controls (P = 0.006). The  risk of TB was 2.3-fold greater in individuals with the T-allele (CT + TT) in comparison to those without (OR 2.3, 95%CI 1.2-4.3, P = 0.006). Mean total sCD14 was significantly increased in the serum of patients with newly diagnosed pulmonary TB (mean ± SD = 3177 ±  751 ng/ml) compared to healthy controls (mean ± SD = 2955 ± 424 ng/ml, P < 0.004).
  
  
  CONCLUSIONS
  These data indicate that the C-159T polymorphism of the CD14 gene is associated with TB; serum sCD14 levels were higher in TB patients in a sample of the Iranian population.
authors.affiliation: Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
authors.forename: R
authors.initials: R
authors.lastname: Alavi-Naini
authors.forename: S
authors.initials: S
authors.lastname: Salimi
authors.forename: B
authors.initials: B
authors.lastname: Sharifi-Mood
authors.forename: A A
authors.initials: AA
authors.lastname: Davoodikia
authors.forename: B
authors.initials: B
authors.lastname: Moody
authors.forename: A
authors.initials: A
authors.lastname: Naghavi
mesh_terms: D015415:Biomarkers / Q000097:blood; D004247:DNA / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007492:Iran / Q000453:epidemiology; D018950:Lipopolysaccharide Receptors / Q000097:blood / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015995:Prevalence; D012189:Retrospective Studies; D014397:Tuberculosis, Pulmonary / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology
chemical_list: D015415:Biomarkers; D018950:Lipopolysaccharide Receptors; D004247:DNA