title: A novel collection of snRNA-like promoters with tissue-specific transcription properties.
abstract: We recently identified a novel dataset of snRNA-like trascriptional units in the human genome. The investigation of a subset of these elements showed that they play relevant roles in physiology and/or pathology. In this work we expand our collection of small RNAs taking advantage of a newly developed algorithm able to identify genome sequence stretches with RNA polymerase (pol) III type 3 promoter features thus constituting putative pol III binding sites. The bioinformatic analysis of a subset of these elements that map in introns of protein-coding genes in antisense configuration suggest their association with alternative splicing, similarly to other recently characterized small RNAs. Interestingly, the analysis of the transcriptional activity of these novel promoters shows that they are active in a cell-type specific manner, in accordance with the emerging body of evidence of a tissue/cell-specific activity of pol III.
authors.affiliation: Department of Experimental Medicine (DiMES), University of Genoa, 16132 Genoa, Italy.
authors.forename: Sonia
authors.initials: S
authors.lastname: Garritano
authors.affiliation: Department of Experimental Medicine (DiMES), University of Genoa, 16132 Genoa, Italy.
authors.forename: Arianna
authors.initials: A
authors.lastname: Gigoni
authors.affiliation: Department of Experimental Medicine (DiMES), University of Genoa, 16132 Genoa, Italy.
authors.forename: Delfina
authors.initials: D
authors.lastname: Costa
authors.affiliation: Department of Experimental Medicine (DiMES), University of Genoa, 16132 Genoa, Italy.|IRCCS-AOU San Martino-IST, Largo Rosanna Benzi 10, 16132 Genoa, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Malatesta
authors.affiliation: Department of Internal Medicine (DIMI), University of Genoa, 16132 Genoa, Italy.|Centre of Excellence for Biomedical research (CEBR), University of Genoa, 16132 Genoa, Italy.
authors.forename: Tullio
authors.identifier: 0000-0002-1819-3811
authors.initials: T
authors.lastname: Florio
authors.affiliation: Department of Experimental Medicine (DiMES), University of Genoa, 16132 Genoa, Italy.|IRCCS-AOU San Martino-IST, Largo Rosanna Benzi 10, 16132 Genoa, Italy.
authors.forename: Ranieri
authors.initials: R
authors.lastname: Cancedda
authors.affiliation: Department of Experimental Medicine (DiMES), University of Genoa, 16132 Genoa, Italy.|IRCCS-AOU San Martino-IST, Largo Rosanna Benzi 10, 16132 Genoa, Italy.
authors.forename: Aldo
authors.initials: A
authors.lastname: Pagano
mesh_terms: D000465:Algorithms; D017398:Alternative Splicing / Q000235:genetics; D001665:Binding Sites / Q000235:genetics; D045744:Cell Line, Tumor; D015894:Genome, Human / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D009928:Organ Specificity / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D012320:RNA Polymerase III / Q000235:genetics*; D012342:RNA, Small Nuclear / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D012342:RNA, Small Nuclear; D016601:RNA-Binding Proteins; D012320:RNA Polymerase III
keywords: RNA polymerase III; alternative splicing; non-coding RNA; small nuclear RNA