title: [Diversity of bacteria isolated from cysts Heterodera glycines in Heilongjiang].
abstract: OBJECTIVE
  To understand the bacterial diversity isolated from the cysts of Heterodera glycines in the soybean field in Heilongjiang Province.
  
  
  METHODS
  Bacteria were isolated from cysts on nutrient agar plates using dilution plate method and further identified by phylogenetic analysis based on 16S rDNA gene sequences.
  
  
  RESULTS
  Totally 90 bacteria strains with different colony morphology were selected on nutrient agar plate and their phylogenetic features were analyzed based on the partial 16S rDNA sequences. In total 7 genera and 22 species were identified, including 46 strains in Gammaproteobacteria (51.1%), 32 in Firmicutes (35.6%), 10 in Betaproteobacteria (11.1%), and 2 in Alphaproteobacteria (2.2%). The dominant bacteria species were Pseudomonas and Bacillus.
  
  
  CONCLUSIONS
  There was abundant species diversity of bacteria isolated from cysts Heterodera glycines in Heilongjiang, and these bacteria may play a physical and ecological roles in nematodes.
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. xiaozhu_1688@126.com
authors.forename: Yingbo
authors.initials: Y
authors.lastname: Zhu
authors.forename: Fengyu
authors.initials: F
authors.lastname: Shi
authors.forename: Niuniu
authors.initials: N
authors.lastname: Wang
authors.forename: Jianqing
authors.initials: J
authors.lastname: Tian
authors.forename: Meichun
authors.initials: M
authors.lastname: Xiang
authors.forename: Xingzhong
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D002681:China; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014431:Tylenchoidea / Q000382:microbiology*
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S