title: Measuring protein synthesis with SUnSET: a valid alternative to traditional techniques?
abstract: Protein synthesis rates commonly are measured using isotopic tracers to quantify the incorporation of a labeled amino acid into muscle proteins. Here we provide evidence supporting our hypothesis that the nonisotopic SUnSET technique is a valid and accurate method for the measurement of in vivo changes in protein synthesis at the whole-muscle and single-muscle fiber levels.
authors.affiliation: Department of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA. cgoodman2@wisc.edu
authors.forename: Craig A
authors.initials: CA
authors.lastname: Goodman
authors.forename: Troy A
authors.initials: TA
authors.lastname: Hornberger
mesh_terms: D000818:Animals; D006801:Humans; D009124:Muscle Proteins / Q000096:biosynthesis*; D018482:Muscle, Skeletal / Q000378:metabolism*; D011500:Protein Synthesis Inhibitors; D011691:Puromycin
chemical_list: D009124:Muscle Proteins; D011500:Protein Synthesis Inhibitors; D011691:Puromycin