title: Mitochondrial D-Loop polymorphism and microsatellite instability in prostate cancer and benign hyperplasia patients.
abstract: In this study mitochondrial D-Loop variations in Iranian prostate cancer and benign prostatic hyperplasia (BPH) patients were investigated. Tumour samples and corresponding non-cancerous prostate tissue from 40 prostate cancer patients and 40 age-matched BPH patients were collected. The entire mtD-loop region (16024-576) was amplified using the PCR method and products were gel-purified and subjected to direct nucleotide sequencing. A total of 129 variations were found, the most frequent being 263A-G and 310T-C among both BPH and prostate cancer patients. Variation of 309 C-T was significantly more frequent in prostate cancer patients (P value<0.05). Four novel variations were observed on comparison with the MITOMAP database. Novel variations were np16154delT, np366G-A, np389G-A and 56insT. There was no correspondence between the different variations and the age of subjects. Considering that D-loop variations were frequent in both BPH and prostate cancer patients in our study, the fact that both groups had high average age can be a possible contributing factor. D-loop polymorphisms and microsatellite instability can influence cell physiology and result in a benign or malignant phenotype. Significantly higher frequency of 309 C-T variation in cancer patients is a notable finding and must be a focus of attention in future studies.
authors.affiliation: Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. ousati@sina.tums.ac.ir
authors.forename: Zahra Ousati
authors.initials: ZO
authors.lastname: Ashtiani
authors.forename: Mansour
authors.initials: M
authors.lastname: Heidari
authors.forename: Sayed-Mohammad
authors.initials: SM
authors.lastname: Hasheminasab
authors.forename: Mohsen
authors.initials: M
authors.lastname: Ayati
authors.forename: Naser
authors.initials: N
authors.lastname: Rakhshani
mesh_terms: D000368:Aged; D004272:DNA, Mitochondrial / Q000235:genetics*; D006801:Humans; D008297:Male; D053842:Microsatellite Instability; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D011379:Prognosis; D011470:Prostatic Hyperplasia / Q000235:genetics* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D004272:DNA, Mitochondrial