title: Cloning of acyl-ACP thioesterase FatA from Arachis hypogaea L. and its expression in Escherichia coli.
abstract: In this study, a full-length cDNA of the acyl-ACP thioesterase, AhFatA, was cloned from developing seeds of Arachis hypogaea L. by 3'-RACE. Sequence analysis showed that the open reading frame encodes a peptide of 372 amino acids and has 50-70% identity with FatA from other plants. Real-time quantitative PCR analysis revealed that AhFatA was expressed in all tissues of A. hypogaea L., but most strongly in the immature seeds harvested at 60 days after pegging. Heterologous expression of AhFatA in Escherichia coli affected bacterial growth and changed the fatty acid profiles of the membrane lipid, resulting in directed accumulation towards palmitoleic acid and oleic acid. These results indicate that AhFatA is at least partially responsible for determining the high palmitoleic acid and oleic acid composition of E. coli.
authors.affiliation: Key Laboratory of Plant Stress, College of Life Science, Shandong Normal University, Ji'nan 250014, China.
authors.forename: Gao
authors.initials: G
authors.lastname: Chen
authors.forename: Zhen-ying
authors.initials: ZY
authors.lastname: Peng
authors.forename: Lei
authors.initials: L
authors.lastname: Shan
authors.forename: Ning
authors.initials: N
authors.lastname: Xuan
authors.forename: Gui-ying
authors.initials: GY
authors.lastname: Tang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lan
authors.initials: L
authors.lastname: Li
authors.forename: Qing-fang
authors.initials: QF
authors.lastname: He
authors.forename: Yu-ping
authors.initials: YP
authors.lastname: Bi
mesh_terms: D010367:Arachis / Q000502:physiology*; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000502:physiology*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism*; D013869:Thiolester Hydrolases / Q000235:genetics* / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D013869:Thiolester Hydrolases; C018748:oleoyl-(acyl-carrier-protein) hydrolase