title: Proteasome inhibitor bortezomi-induced the apoptosis of laryngeal squamous cell carcinoma Hep-2 cell line via disrupting redox equilibrium.
abstract: Bortezomib, a proteasome inhibitor, has been therapeutic effects in some solid tumors. In the present work, the effects of bortezomib on laryngeal squamous cell carcinoma Hep-2 cell line was investigated and the role of redox equilibrium was explored. The results showed that bortezomib decreased the cell viability and increased the apoptosis in Hep-2 cells. In addition, bortezomib overproduced reactive oxygen species (ROS) and induced the loss of mitochondrial membrane potential (ΔΨ(m)). And the activity of caspase-3 increased. N-acetyl-L-cysteine (L-NAC), a ROS scavenger, alleviated oxidative stress and inhibited the apoptosis induced by bortezomib. However, buthionine sulfoximine (BSO), an inhibitor of GSH synthetase, aggravated bortezomib-induced oxidative stress and apoptosis. So bortezomi-induced the apoptosis of Hep-2 cells via disrupting redox equilibrium.
authors.affiliation: Jining Medical University, Jining City, Shandong Province, PR China. yangxinxin1979@yahoo.com.cn
authors.forename: Xinxin
authors.initials: X
authors.lastname: Yang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001897:Boronic Acids / Q000494:pharmacology*; D000069286:Bortezomib; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology; D053148:Caspase 3 / Q000378:metabolism; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000473:pathology; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D061988:Proteasome Inhibitors / Q000494:pharmacology*; D011719:Pyrazines / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D001897:Boronic Acids; D061988:Proteasome Inhibitors; D011719:Pyrazines; D017382:Reactive Oxygen Species; D000069286:Bortezomib; D053148:Caspase 3