title: Membrane-protein binding measured with solution-phase plasmonic nanocube sensors.
abstract: We describe a solution-phase sensor of lipid-protein binding based on localized surface plasmon resonance (LSPR) of silver nanocubes. When silica-coated nanocubes are mixed in a suspension of lipid vesicles, supported membranes spontaneously assemble on their surfaces. Using a standard laboratory spectrophotometer, we calibrated the LSPR peak shift due to protein binding to the membrane surface and then characterized the lipid-binding specificity of a pleckstrin homology domain protein.
authors.affiliation: Howard Hughes Medical Institute, University of California, Berkeley, California, USA.
authors.forename: Hung-Jen
authors.initials: HJ
authors.lastname: Wu
authors.forename: Joel
authors.initials: J
authors.lastname: Henzie
authors.forename: Wan-Chen
authors.initials: WC
authors.lastname: Lin
authors.forename: Christopher
authors.initials: C
authors.lastname: Rhodes
authors.forename: Zhu
authors.initials: Z
authors.lastname: Li
authors.forename: Elodie
authors.initials: E
authors.lastname: Sartorel
authors.forename: Jeremy
authors.initials: J
authors.lastname: Thorner
authors.forename: Peidong
authors.initials: P
authors.lastname: Yang
authors.forename: Jay T
authors.initials: JT
authors.lastname: Groves
mesh_terms: D002138:Calibration; D008051:Lipid Bilayers / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D011485:Protein Binding; D012822:Silicon Dioxide; D012834:Silver / Q000737:chemistry; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D020349:Surface Plasmon Resonance
chemical_list: D008051:Lipid Bilayers; D008565:Membrane Proteins; D012996:Solutions; D012834:Silver; D012822:Silicon Dioxide