title: Anti-inflammatory effect and modulation of cytochrome P450 activities by Artemisia annua tea infusions in human intestinal Caco-2 cells.
abstract: In an attempt to understand the beneficial health effects of Artemisia annua other than its anti-malaria properties, extracts from different cultivars prepared as tea infusions were investigated using Caco-2 cells on the intestinal inflammation and cytochrome P450 (CYP) activities. The characterisation of their phenolic compound (PC) profile revealed rosmarinic and chlorogenic acids as the main PCs. The extracts, assayed on Caco-2 cells at a plausible intestinal concentration, significantly decreased the secretion of pro-inflammatory cytokines, IL-8 and IL-6. This effect could be attributable at least to their content in rosmarinic acid, detected as a potent anti-inflammatory compound. The extracts also inhibited the activity of CYP3A4, whose expression was induced by 1,25-dihydroxyvitamin D(3), and of CYP1A1, induced by benzo(a)pyrene. Our results highlight the advantage of drinking A. annua infusions for their potent anti-inflammatory effect, linked to PC content, which could synergise their antimalarial activity.
authors.affiliation: Institut des Sciences de la Vie & UCLouvain, Croix du Sud, 1348 Louvain-la-Neuve, Belgium.
authors.forename: Pedro
authors.initials: P
authors.lastname: Melillo de Magalhães
authors.forename: Isabelle
authors.initials: I
authors.lastname: Dupont
authors.forename: Aurélie
authors.initials: A
authors.lastname: Hendrickx
authors.forename: Aurélie
authors.initials: A
authors.lastname: Joly
authors.forename: Thomas
authors.initials: T
authors.lastname: Raas
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Dessy
authors.forename: Thérèse
authors.initials: T
authors.lastname: Sergent
authors.forename: Yves-Jacques
authors.initials: YJ
authors.lastname: Schneider
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D031172:Artemisia annua / Q000737:chemistry*; D018938:Caco-2 Cells; D002726:Chlorogenic Acid / Q000494:pharmacology; D019363:Cytochrome P-450 CYP1A1 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051544:Cytochrome P-450 CYP3A / Q000378:metabolism; D065692:Cytochrome P-450 CYP3A Inhibitors; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D015850:Interleukin-6 / Q000276:immunology; D016209:Interleukin-8 / Q000037:antagonists & inhibitors / Q000276:immunology; D007422:Intestines / Q000201:enzymology* / Q000276:immunology; D010636:Phenols / Q000494:pharmacology; D028321:Plant Preparations / Q000494:pharmacology*; D000093242:Interleukin-6 Inhibitors
chemical_list: D000893:Anti-Inflammatory Agents; D002726:Chlorogenic Acid; D019363:Cytochrome P-450 CYP1A1; D051544:Cytochrome P-450 CYP3A; D065692:Cytochrome P-450 CYP3A Inhibitors; D004791:Enzyme Inhibitors; D015850:Interleukin-6; D016209:Interleukin-8; D010636:Phenols; D028321:Plant Preparations; D000093242:Interleukin-6 Inhibitors; C510163:CYP3A4 protein, human