title: Non-suture technique for rabbit oviduct anastomosis with 2-octyl cyanoacrylate: a histopathologic and biomechanical analysis.
abstract: OBJECTIVE
  The aim of this study was to investigate histological and biomechanical properties of oviduct anastomosis with 2-octyl cyanoacrylate (OCA) in the rabbit.
  
  
  METHODS
  Sixty female rabbits were randomly divided equally into three groups: A (control), B (traditional catgut suture), and C (non-suture technique using OCA). After suture or OCA anastomosis, gross examination (adhesion formation) and histopathology (hematoxylin-eosin), ultrastructure (transmission electron microscopy), and biomechanics (bursting pressure) on para-anastomotic site were investigated on oviduct taken at 1 (A1, B1, C1) and 4 (A2, B2, C2) weeks, respectively.
  
  
  RESULTS
  Adhesion score in group B was more severe than that in groups A and C at 1 and 4 weeks. Histopathology showed that acute endosalpingitis in group B was the most intense at 1 week, followed by significantly more tissue stimulation induced by catgut and foreign-body giant cells in group B than in group C at 4 weeks. Ultrastructural damage of ciliated cells was reversed partly (B2) and completely (C2) at 4 weeks. Bursting pressure in C1 was weaker than that in B1, followed by no significant difference at 4 weeks.
  
  
  CONCLUSIONS
  Non-suture using OCA for oviduct anastomosis can be accepted as a new-perspective technique.
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou, Zhejiang, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Yang
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Li
authors.forename: Hu-Xiang
authors.initials: HX
authors.lastname: Zhang
authors.forename: Fei-Yun
authors.initials: FY
authors.lastname: Zheng
mesh_terms: D000714:Anastomosis, Surgical / Q000009:adverse effects / Q000379:methods*; D000818:Animals; D001696:Biomechanical Phenomena; D003487:Cyanoacrylates / Q000627:therapeutic use*; D005260:Female; D010057:Oviducts / Q000601:surgery* / Q000648:ultrastructure; D011817:Rabbits; D013314:Stress, Mechanical; D000267:Tissue Adhesions / Q000209:etiology; D014014:Tissue Adhesives / Q000627:therapeutic use*
chemical_list: D003487:Cyanoacrylates; D014014:Tissue Adhesives; C100832:octyl 2-cyanoacrylate