title: PCR detection of Haemophilus influenzae from respiratory specimens.
abstract: The detection of Haemophilus influenzae by conventional methods like culture is time-consuming and may give false-negative results, especially during ongoing antibiotic treatment. Therefore, non-culture based methods that are sensitive, specific, and rapid are valuable for early diagnosis and effective therapy. Here we describe a quantitative real-time PCR assay based on the outer membrane P6 gene omp6, to detect H. influenzae and its application on respiratory tract specimens.
authors.affiliation: Department of Clinical Microbiology, Uppsala, University Hospital, Uppsala, Sweden.
authors.forename: Guma M K
authors.initials: GM
authors.lastname: Abdeldaim
authors.forename: Björn
authors.initials: B
authors.lastname: Herrmann
mesh_terms: D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D004269:DNA, Bacterial / Q000302:isolation & purification; D006192:Haemophilus Infections / Q000175:diagnosis; D006193:Haemophilus influenzae / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D017817:Nasal Lavage Fluid / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D012015:Reference Standards; D013183:Sputum / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial