title: Properties of the electrogenic activity of bacteriorhodopsin.
abstract: In this study, we analyzed the photoelectric current generated by bacteriorhodopsin adsorbed on a polymer film, "Lumirror" (Muneyuki et al. in FEBS Lett 427:109-114, 1998). We could examine the photoelectric current over a wide range of light intensity and pH values using the same membrane owing to the mechanical and chemical stability of the thin polymer film. We analyzed the photoelectric current by comparison with a simple equivalent electric circuit. Analysis of experimental results obtained at different light intensities suggested that the electromotive force of the bacteriorhodopsin was independent of light intensity. The pH dependence of the photoelectric current suggested that the bacteriorhodopsin could generate a maximum electromotive force at approximately pH 6.
authors.affiliation: Department of Physics, Faculty of Science and Engineering, Chuo University, Tokyo 112-8551, Japan.
authors.forename: Shizuma
authors.initials: S
authors.lastname: Miyazaki
authors.forename: Makoto
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Søren Bo
authors.initials: SB
authors.lastname: Brier
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Higaki
authors.forename: Takumi
authors.initials: T
authors.lastname: Yamada
authors.forename: Tetsuaki
authors.initials: T
authors.lastname: Okamoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ueno
authors.forename: Shoichi
authors.initials: S
authors.lastname: Toyabe
authors.forename: Eiro
authors.initials: E
authors.lastname: Muneyuki
mesh_terms: D000327:Adsorption; D001436:Bacteriorhodopsins / Q000737:chemistry / Q000378:metabolism*; D004307:Dose-Response Relationship, Radiation; D004553:Electric Conductivity; D006863:Hydrogen-Ion Concentration; D008027:Light
chemical_list: D001436:Bacteriorhodopsins