title: Quantification and purification of mangiferin from Chinese Mango (Mangifera indica L.) cultivars and its protective effect on human umbilical vein endothelial cells under H(2)O(2)-induced stress.
abstract: Mangiferin is a natural xanthonoid with various biological activities. Quantification of mangiferin in fruit peel, pulp, and seed kernel was carried out in 11 Chinese mango (Mangifera indica L.) cultivars. The highest mangiferin content was found in the peel of Lvpimang (LPM) fruit (7.49 mg/g DW). Efficient purification of mangiferin from mango fruit peel was then established for the first time by combination of macroporous HPD100 resin chromatography with optimized high-speed counter-current chromatography (HSCCC). Purified mangiferin was identified by both HPLC and LC-MS, and it showed higher DPPH(•) free-radical scavenging capacities and ferric reducing ability of plasma (FRAP) than by l-ascorbic acid (Vc) or Trolox. In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H(2)O(2)-induced stress. Cells treated with mangiferin resulted in significant enhanced cell survival under of H(2)O(2) stress. Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases.
authors.affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
authors.forename: Fenglei
authors.initials: F
authors.lastname: Luo
authors.affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lv
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Horticulture, South China Agricultural University, Guangzhou 510642, Guangdong, China.
authors.forename: Guibing
authors.initials: G
authors.lastname: Hu
authors.affiliation: Guangxi Subtropical Crop Research Institute, Nanning 530001, Guangxi, China.
authors.forename: Guodi
authors.initials: G
authors.lastname: Huang
authors.affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
authors.forename: Jiukai
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
authors.forename: Chongde
authors.initials: C
authors.lastname: Sun
authors.affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
authors.forename: Kunsong
authors.initials: K
authors.lastname: Chen
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000737:chemistry / Q000378:metabolism*; D005638:Fruit / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D031022:Mangifera / Q000737:chemistry / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism; D044004:Xanthones / Q000378:metabolism*
chemical_list: D016166:Free Radical Scavengers; D010936:Plant Extracts; D044004:Xanthones; C013592:mangiferin; D006861:Hydrogen Peroxide
keywords: HUVEC; Mangifera indica; antioxidant; mangiferin; protective effect; purification