title: Evidence for natural recombination between mink enteritis virus and canine parvovirus.
abstract: A virus was isolated from mink showing clinical and pathological signs of enteritis in China. This virus, designated MEV/LN-10, was identified as mink enteritis virus (MEV) based on its cytopathic effect in the feline F81 cell line, the hemagglutination (HA) and hemagglutination inhibition (HI) assay, electron microscopy (EM) and animal infection experiments. The complete viral genome was cloned and sequenced. Phylogenetic and recombination analyses on the complete MEV/LN-10 genome showed evidence of recombination between MEV and canine parvovirus (CPV). The genome was composed of the NS1 gene originating from CPV while the VP1 gene was of MEV origin. This is the first demonstration of recombination between a CPV and MEV in nature. Our findings not only provide valuable evidence indicating that recombination is an important genetic mechanism contributing to the variation and evolution of MEV, but also that heterogeneous recombination can occur in the feline parvovirus subspecies.
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, 130112, China.
authors.forename: Jianke
authors.initials: J
authors.lastname: Wang
authors.forename: Shipeng
authors.initials: S
authors.lastname: Cheng
authors.forename: Li
authors.initials: L
authors.lastname: Yi
authors.forename: Yuening
authors.initials: Y
authors.lastname: Cheng
authors.forename: Shen
authors.initials: S
authors.lastname: Yang
authors.forename: Hongli
authors.initials: H
authors.lastname: Xu
authors.forename: Hang
authors.initials: H
authors.lastname: Zhao
authors.forename: Xijun
authors.initials: X
authors.lastname: Yan
authors.forename: Hua
authors.initials: H
authors.lastname: Wu
mesh_terms: D000818:Animals; D002415:Cats; D002460:Cell Line; D002681:China; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D004751:Enteritis / Q000662:veterinary; D016679:Genome, Viral; D006385:Hemagglutination Inhibition Tests; D008854:Microscopy, Electron; D008907:Mink; D053490:Mink enteritis virus / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D017993:Parvovirus, Canine / Q000235:genetics*; D010802:Phylogeny; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014771:Virion / Q000648:ultrastructure
chemical_list: D004279:DNA, Viral