title: Hypochlorite digestion method for efficient recovery of PHB from Alcaligenes faecalis.
abstract: We reported the optimum amount of PHB accumulated by Alcaligenes faecalis during its 24 h growth under nitrogen deficient conditions. After 24 h incubation decrease in the amount of PHB was recorded. Hypochlorite digestion of biomass of organism followed by extraction with a solvent system consisting of 1:1 mixture of ethanol and acetone resulted in efficient recovery of PHB vis-à-vis earlier methods. This solvent system gave a high recovery yield, i.e. 5.6 gL(-1) vis-à-vis earlier reported yield, 1.34 gL(-1) (by same method), 0.63 gL(-1) (by chloroform extraction method) and 1.1 gL(-1) (by dispersion method).
authors.affiliation: Department of Microbiology, PSGVP Mandal's ASC College Shahada, Maharashtra, 425 409 India.
authors.forename: R Z
authors.initials: RZ
authors.lastname: Sayyed
authors.forename: N S
authors.initials: NS
authors.lastname: Gangurde
authors.forename: S B
authors.initials: SB
authors.lastname: Chincholkar
keywords: Alcaligenes faecalis; Hypochlorite; NPCM; PHB