title: β2-Adrenergic receptor solutions for structural biology analyzed with microscale NMR diffusion measurements.
abstract: Microcoil NMR measurements were performed to determine the final composition of solutions of the β(2)-adrenergic receptor (β(2)AR) reconstituted with a detergent and to study the hydrodynamic properties of the detergent micelles containing β(2)AR. Standards are established for the reproducible preparation of G-protein-coupled receptor solutions for crystallization trials and solution NMR studies.
authors.affiliation: Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors.forename: Reto
authors.initials: R
authors.lastname: Horst
authors.forename: Pawel
authors.initials: P
authors.lastname: Stanczak
authors.forename: Raymond C
authors.initials: RC
authors.lastname: Stevens
authors.forename: Kurt
authors.initials: K
authors.lastname: Wüthrich
mesh_terms: D004058:Diffusion; D006801:Humans; D008823:Micelles; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D018343:Receptors, Adrenergic, beta-2 / Q000737:chemistry*; D012996:Solutions
chemical_list: D008823:Micelles; D018343:Receptors, Adrenergic, beta-2; D012996:Solutions