title: Nucleic acids electro-transfer: from bench to bedside.
abstract: There are a number of stigmas attached to the development of antitumor drugs such as their safe and efficient delivery into target cells or tissues. The one such case is that of macromolecules, such as nucleic acids where it poses severe limits. From this point of view, electro-pulsation proves to be a promising method for cancer-associated gene therapy. It involves the direct application of electric pulses on cells or tissues which leads to a transient permeabilization of their plasma membrane allowing efficient in vitro and in vivo delivery of exogenous molecules. The present review probes the electrotransfer of nucleic acids, the nature of nucleic acids (plasmid DNA, mRNA, siRNA, LNA...) which can be electrotransferred and the mechanism of their electrotransfer.
authors.affiliation: CNRS, Institut de Pharmacologie et de Biologie Structurale, BP 64182, 205 route de Narbonne, F-31077 Toulouse, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Chabot
authors.forename: Christelle
authors.initials: C
authors.lastname: Rosazza
authors.forename: Muriel
authors.initials: M
authors.lastname: Golzio
authors.forename: Andreas
authors.initials: A
authors.lastname: Zumbusch
authors.forename: Justin
authors.initials: J
authors.lastname: Teissié
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Rols
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability; D004247:DNA / Q000008:administration & dosage*; D018274:Electroporation; D006801:Humans; D010957:Plasmids; D012313:RNA / Q000008:administration & dosage*
chemical_list: D012313:RNA; D004247:DNA