title: Mammalian mediator 19 mediates H1299 lung adenocarcinoma cell clone conformation, growth, and metastasis.
abstract: Mammalian mediator (MED) is a multi-protein coactivator that has been identified by several research groups. The involvement of the MED complex subunit 19 (MED 19) in the metastasis of lung adenocarcinoma cell line (H1299), which expresses the MED 19 subunit, was here investigated. When MED 19 expression was decreased by RNA interference H1299 cells demonstrated reduced clone formation, arrest in the S phase of the cell cycle, and lowered metastatic capacity. Thus, MED 19 appears to play important roles in the biological behavior of non-small cell lung carcinoma cells. These findings may be important for the development of novel lung carcinoma treatments.
authors.affiliation: Department of Respiratory Medicine, First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Lu-Lu
authors.initials: LL
authors.lastname: Xu
authors.forename: Shu-Liang
authors.initials: SL
authors.lastname: Guo
authors.forename: Su-Ren
authors.initials: SR
authors.lastname: Ma
authors.forename: Yong-Ai
authors.initials: YA
authors.lastname: Luo
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism / Q000556:secondary*; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells / Q000378:metabolism / Q000473:pathology; D005434:Flow Cytometry; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D056892:Mediator Complex / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D016196:S Phase / Q000502:physiology*; D014407:Tumor Cells, Cultured
chemical_list: C558319:MED19 protein, human; D056892:Mediator Complex; D034741:RNA, Small Interfering