title: High-throughput simultaneous analysis of pesticides by supercritical fluid chromatography/tandem mass spectrometry.
abstract: Combination techniques such as gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS) are commonly used for pesticide residue analysis, but there is no reported method for the simultaneous analysis of multiple pesticides in a sample using a single instrument. Supercritical fluid chromatography (SFC) offers high resolution at high flow rates and various separation modes and hence may aid the rapid simultaneous analysis of pesticide. We developed an SFC/MS/MS method and analyzed 17 pesticides with a wide range of polarities (logP(ow)=-4.6 to 7.05) and molecular weights (112.1-888.6) within 11min using a polar-embedded reversed-phase column. To the best of our knowledge, there is no previous report on the SFC analysis of a wide variety of compounds, including highly hydrophilic ones. By SFC, diquat dibromide (logP(ow)=-4.6), together with cypermethrin (logP(ow)=6.6) and tralomethrin (logP(ow)=5.05), could be detected in the presence of various other pesticides using a single mobile phase. SFC/MS allows for the rapid and simultaneous analysis of low concentrations (ng/L levels) of pesticides that typically need to be analyzed by GC/MS and LC/MS separately.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Ishibashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Ando
authors.forename: Miho
authors.initials: M
authors.lastname: Sakai
authors.forename: Atsuki
authors.initials: A
authors.lastname: Matsubara
authors.forename: Takato
authors.initials: T
authors.lastname: Uchikata
authors.forename: Eiichiro
authors.initials: E
authors.lastname: Fukusaki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Bamba
mesh_terms: D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods*; D012275:Oryza / Q000737:chemistry; D010573:Pesticide Residues / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014675:Vegetables / Q000737:chemistry
chemical_list: D010573:Pesticide Residues