title: PNA-based microRNA inhibitors elicit anti-inflammatory effects in microglia cells.
abstract: Peptide nucleic acid (PNA) inhibitors of miR-221-3p (CU-PNA-221) and miR-466l-3p (CU-PNA-466) demonstrated changes in inflammatory responses. Suppression of inflammatory signalling was unexpected and further investigation led to the identification of calmodulin as a novel target of miRNA-466l-3p. These studies demonstrate that exogenous agents may suppress neuroinflammation mediated by microglial cells.
authors.affiliation: Department of Chemistry & Biochemistry and the BioFrontiers Institute, University of Colorado at Boulder, Boulder, Colorado 80303, USA.
authors.forename: Peter N
authors.initials: PN
authors.lastname: Brown
authors.forename: Hang
authors.initials: H
authors.lastname: Yin
mesh_terms: D000893:Anti-Inflammatory Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D001483:Base Sequence; D002147:Calmodulin / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D017628:Microglia / Q000166:cytology / Q000187:drug effects; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D002147:Calmodulin; D035683:MicroRNAs; D020135:Peptide Nucleic Acids; D012333:RNA, Messenger; D016753:Interleukin-10; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II