title: An image skeletonization-based tool for pollen tube morphology analysis and phenotyping.
abstract: The mechanism underlying pollen tube growth involves diverse genes and molecular pathways. Alterations in the regulatory genes or pathways cause phenotypic changes reflected by cellular morphology, which can be captured using fluorescence microscopy. Determining and classifying pollen tube morphological phenotypes in such microscopic images is key to our understanding the involvement of genes and pathways. In this context, we propose a computational method to extract quantitative morphological features, and demonstrate that these features reflect morphological differences relevant to distinguish different defects of pollen tube growth. The corresponding software tool furthermore includes a novel semi-automated image segmentation approach, allowing to highly accurately identify the boundary of a pollen tube in a microscopic image.
authors.affiliation: CAS-MPG Partner Institute and CAS Key Laboratory for Computational Biology, Shanghai Institutes for Biological Sciences, The Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Chaofeng
authors.initials: C
authors.lastname: Wang
authors.forename: Cai-Ping
authors.initials: CP
authors.lastname: Gui
authors.forename: Hai-Kuan
authors.initials: HK
authors.lastname: Liu
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.forename: Axel
authors.initials: A
authors.lastname: Mosig
mesh_terms: D003198:Computer Simulation; D017343:Genes, Plant; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D010641:Phenotype; D053205:Pollen Tube / Q000033:anatomy & histology*; D014026:Nicotiana / Q000033:anatomy & histology* / Q000235:genetics