title: [Effects of hydrogen sulfide preconditioning on myocardial ischemia reperfusion injury in rats].
abstract: OBJECTIVE
  To investigate the effects of hydrogen sulfide preconditioning on myocardial ischemia reperfusion injury in rats.
  
  
  METHODS
  Sprague-Dawley male rats were divided into 4 groups with 10 in each group: in S group rats received sham operation; in IR group rats were given with NS (1.0 ml/kg iv) 24 h before ischemia; in H group rats were treated with NaHS (0.05 mg/kg iv) 24 h before ischemia; and in D group, NaHS-treated rats received 5-hydroxydecanoate (5-HD) 15 min before ischemia. Rats in IR group,H group and D group were subjected to ischemia by occlusion of coronary artery for 30 min followed by 2 h of reperfusion. At the end of the reperfusion,myocardial infarct size was measured. SAM-s was measured by Western blotting. Plasma SOD activity and MDA were determined at the end of reperfusion.
  
  
  RESULTS
  The infarct size was significantly lesser in H group (25.40 % ± 3.54%) than that in IR group (38.27% ±5.64%,P<0.05). The SAM-s protein expression in myocardium was significantly lower in H group than that in IR group. The plasma MDA content was significantly lower and SOD activity was higher in H group than those in IR group,but there was no difference between IR group and D group.
  
  
  CONCLUSIONS
  The hydrogen sulfide preconditioning attenuates myocardial IR injury possibly through down-regulating SAM-s expression,reducing the production of oxygen free radicals and enhancing anti-oxidize effect in rats.
authors.affiliation: Department of Anesthesiology, Central South University, Changsha, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Ke
authors.initials: K
authors.lastname: Ran
authors.forename: Zheng-guo
authors.initials: ZG
authors.lastname: Tang
authors.forename: Shuang-feng
authors.initials: SF
authors.lastname: Li
authors.forename: Ye-tian
authors.initials: YT
authors.lastname: Chang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D019157:Ischemic Preconditioning, Myocardial; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D006862:Hydrogen Sulfide