title: Ultrastructural observation and gene expression profiling of Schistosoma japonicum derived from two natural reservoir hosts, water buffalo and yellow cattle.
abstract: Water buffalo and yellow cattle are the two of the most important natural reservoir hosts for Schistosoma japonicum in endemic areas of China, although their susceptibility differs, with water buffalo being less conducive to the growth and development of S. japonicum. Results from the current study show that the general morphology and ultrastructure of adult schistosomes derived from the two hosts also differed. Using high-throughput microarray technology, we also compared the gene expression profiles of adult schistosomes derived from the two hosts. We identified genes that were differentially expressed in worms from the two natural hosts. Further analysis revealed that genes associated with protein kinase and phosphatase, the stimulus response, and lipid and nucleotide metabolism were overexpressed, whereas genes associated with reproduction, anatomical structure morphogenesis and multifunctional motif were underexpressed in schistosomes from water buffalo. These differentially expressed genes were mainly involved in nucleotide, energy, lipid metabolism, energy metabolism, transcription, transport and signaling pathway. This suggests that they are key molecules affecting the survival and development of schistosomes in different natural host species. The results of this study add to current understanding of the interplay between parasites and their natural hosts, and provide valuable information for the screening of vaccine candidates or new drug targets against schistosomiasis in the natural reservoir hosts in endemic areas.
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai, People's Republic of China.
authors.forename: Jianmei
authors.initials: J
authors.lastname: Yang
authors.forename: Xingang
authors.initials: X
authors.lastname: Feng
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Fu
authors.forename: Chunxiu
authors.initials: C
authors.lastname: Yuan
authors.forename: Yang
authors.initials: Y
authors.lastname: Hong
authors.forename: Yaojun
authors.initials: Y
authors.lastname: Shi
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Jinming
authors.initials: J
authors.lastname: Liu
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Ke
authors.initials: K
authors.lastname: Lu
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Lin
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000382:microbiology*; D002417:Cattle; D020869:Gene Expression Profiling / Q000379:methods*; D012549:Schistosoma japonicum / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure*