title: Genotypic variability among soybean genotypes under NaCl stress and proteome analysis of salt-tolerant genotype.
abstract: The present investigation was conducted to evaluate salt tolerance in ten genotypes of soybean (Glycine max L.). Twelve-day-old seedlings, grown hydroponically, were treated with 0, 25, 50, 75, 100, 125 and 150 mM NaCl for 10 days. Growth, lipid peroxidation and antioxidant enzyme activities were evaluated. Growth, measured in terms of length, fresh weight and dry weight of plants, was drastically reduced in Pusa-24 while there was little effect of NaCl treatment on Pusa-37 genotype of soybean. High level of lipid peroxidation was observed in Pusa-24 as indicated by increased level of malondialdehyde. Activities of superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase were maximum in Pusa-37 where 9-, 1-, 5- and 6-fold increase over control were observed, respectively. The results suggested that Pusa-24 and Pusa-37 are salt-sensitive and salt-tolerant genotype of soybean, respectively, and antioxidant defence system is involved in conferring the sensitiveness and tolerance in these genotypes. Salt-tolerant genotype Pusa-37, was further analysed by 2-dimensional gel electrophoresis to analyse the differential expression of proteins at high salt stress. In the present study, 173 protein spots were identified. Of these, 40 proteins were responsive to salinity in that they were either up- or downregulated. This study could help us in identifying the possible regulatory switches (gene/s) controlling novel proteins of the salt-tolerant genotype of the crop plants and their possible role in defence mechanism.
authors.affiliation: Department of Botany, Faculty of Science, Jamia Hamdard, New Delhi 110062, India. kur.hakeem@gmail.com
authors.forename: Khalid Rehman
authors.initials: KR
authors.lastname: Hakeem
authors.forename: Faheema
authors.initials: F
authors.lastname: Khan
authors.forename: Ruby
authors.initials: R
authors.lastname: Chandna
authors.forename: Tariq Omer
authors.initials: TO
authors.lastname: Siddiqui
authors.forename: Muhammad
authors.initials: M
authors.lastname: Iqbal
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D005838:Genotype; D015227:Lipid Peroxidation / Q000187:drug effects / Q000235:genetics; D011392:Proline / Q000378:metabolism; D040901:Proteomics; D055049:Salt Tolerance / Q000187:drug effects / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology*; D013025:Glycine max / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D013312:Stress, Physiological / Q000187:drug effects* / Q000235:genetics
chemical_list: D000975:Antioxidants; D012965:Sodium Chloride; D011392:Proline