title: Staphylococcus aureus and Escherichia coli have disparate dependences on KsgA for growth and ribosome biogenesis.
abstract: BACKGROUND
  The KsgA methyltransferase has been conserved throughout evolution, methylating two adenosines in the small subunit rRNA in all three domains of life as well as in eukaryotic organelles that contain ribosomes. Understanding of KsgA's important role in ribosome biogenesis has been recently expanded in Escherichia coli; these studies help explain why KsgA is so highly conserved and also suggest KsgA's potential as an antimicrobial drug target.
  
  
  RESULTS
  We have analyzed KsgA's contribution to ribosome biogenesis and cell growth in Staphylococcus aureus. We found that deletion of ksgA in S. aureus led to a cold-sensitive growth phenotype, although KsgA was not as critical for ribosome biogenesis as it was shown to be in E. coli. Additionally, the ksgA knockout strain showed an increased sensitivity to aminoglycoside antibiotics. Overexpression of a catalytically inactive KsgA mutant was deleterious in the knockout strain but not the wild-type strain; this negative phenotype disappeared at low temperature.
  
  
  CONCLUSIONS
  This work extends the study of KsgA, allowing comparison of this aspect of ribosome biogenesis between a Gram-negative and a Gram-positive organism. Our results in S. aureus are in contrast to results previously described in E. coli, where the catalytically inactive protein showed a negative phenotype in the presence or absence of endogenous KsgA.
authors.affiliation: Department of Physiology and Molecular Biophysics, Virginia Commonwealth University, Richmond, VA, USA.
authors.forename: Heather C
authors.initials: HC
authors.lastname: O'Farrell
authors.forename: Jason P
authors.initials: JP
authors.lastname: Rife
mesh_terms: D000617:Aminoglycosides / Q000633:toxicity; D000900:Anti-Bacterial Agents / Q000633:toxicity; D003080:Cold Temperature; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D017353:Gene Deletion; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D013211:Staphylococcus aureus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000502:physiology*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D008780:Methyltransferases; C047346:16S rRNA (adenine(1518)-N(6)-adenine(1519)-N(6))-dimethyltransferase