title: Optimization of fermentation medium for the production of atrazine degrading strain Acinetobacter sp. DNS(32) by statistical analysis system.
abstract: Statistical experimental designs provided by statistical analysis system (SAS) software were applied to optimize the fermentation medium composition for the production of atrazine-degrading Acinetobacter sp. DNS(32) in shake-flask cultures. A "Plackett-Burman Design" was employed to evaluate the effects of different components in the medium. The concentrations of corn flour, soybean flour, and K(2)HPO(4) were found to significantly influence Acinetobacter sp. DNS(32) production. The steepest ascent method was employed to determine the optimal regions of these three significant factors. Then, these three factors were optimized using central composite design of "response surface methodology." The optimized fermentation medium composition was composed as follows (g/L): corn flour 39.49, soybean flour 25.64, CaCO(3) 3, K(2)HPO(4) 3.27, MgSO(4)·7H(2)O 0.2, and NaCl 0.2. The predicted and verifiable values in the medium with optimized concentration of components in shake flasks experiments were 7.079 × 10(8) CFU/mL and 7.194 × 10(8) CFU/mL, respectively. The validated model can precisely predict the growth of atrazine-degraing bacterium, Acinetobacter sp. DNS(32).
authors.affiliation: School of Resources and Environment, Northeast Agricultural University, Harbin 150030, China. zhangyinghr@hotmail.com
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Wang
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.forename: Huo-Sheng
authors.initials: HS
authors.lastname: Guo
authors.forename: Dong-Fang
authors.initials: DF
authors.lastname: Meng
authors.forename: Po-Keung
authors.initials: PK
authors.lastname: Wong
mesh_terms: D000150:Acinetobacter / Q000145:classification / Q000378:metabolism*; D001280:Atrazine / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D003198:Computer Simulation; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism*; D003627:Data Interpretation, Statistical; D008954:Models, Biological; D015233:Models, Statistical; D013045:Species Specificity
chemical_list: D003470:Culture Media; D001280:Atrazine