title: Effects of salvianolic acid B on survival, self-renewal and neuronal differentiation of bone marrow derived neural stem cells.
abstract: Our previous study has demonstrated the therapeutic potential of bone marrow derived-neural stem cells (BM-NSCs) in CNS disorders; however, the beneficial effects are modest due to the poor survival and low neural differentiation frequency. Here, we demonstrate that salvianolic acid B (Sal B), a potent aqueous of a well known Chinese medicine herb, Salvia miltiorrhiza, possesses the ability to promote BM-NSCs proliferation in a dose dependent manner as verified by growth curve and Bromodeoxyuridine (BrdU) incorporation assays; While in differentiation medium, Sal B promoted nestin(+) BM-NSCs differentiated into greater numbers of NF-M(+) neurons and NG2(+) oligodendrocyte precursors, but fewer GFAP(+) astrocytes as verified by triple immunostaining and quantitative analysis; upon exposure to H(2)O(2), Sal B facilitated the cells survival, reduced LDH leakage, and inhibited apoptosis, displaying a dose-dependent neuroprotective effect on BM-NSCs. Sal B induced brain-derived neurotrophic factor (BDNF) production by BM-NSCs, which may be beneficial for the cells survival and differentiation in unfavourable environment. The collective evidence indicates that Sal B may be a potential drug to upgrade the therapeutic efficiency of BM-NSCs in CNS diseases.
authors.affiliation: School of Pharmacy, Liaoning University of Traditional Chinese Medicine, 77 Sheng Ming Yi Lu, Dalian, Liaoning 116600, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.forename: Tingguo
authors.initials: T
authors.lastname: Kang
authors.forename: Yang
authors.initials: Y
authors.lastname: Xia
authors.forename: Qingping
authors.initials: Q
authors.lastname: Wen
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Zhang
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.forename: Yv
authors.initials: Y
authors.lastname: Hu
authors.forename: Haiguang
authors.initials: H
authors.lastname: Hao
authors.forename: Dan
authors.initials: D
authors.lastname: Zhao
authors.forename: Dong
authors.initials: D
authors.lastname: Sun
authors.forename: Yaping
authors.initials: Y
authors.lastname: Yan
authors.forename: Guang-Xian
authors.initials: GX
authors.lastname: Zhang
authors.forename: Jingxian
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D000941:Antigens / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D001572:Benzofurans / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D019610:Cytoprotection; D004305:Dose-Response Relationship, Drug; D005904:Glial Fibrillary Acidic Protein; D006861:Hydrogen Peroxide / Q000633:toxicity; D007150:Immunohistochemistry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000378:metabolism; D058953:Neural Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D016900:Neurofilament Proteins / Q000378:metabolism; D055495:Neurogenesis / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016877:Oxidants / Q000633:toxicity; D010641:Phenotype; D011509:Proteoglycans / Q000378:metabolism; D018874:Spheroids, Cellular; D013997:Time Factors; D000099216:Chondroitin Sulfate Proteoglycan 4; D053630:Depsides
chemical_list: D000941:Antigens; D001572:Benzofurans; D015415:Biomarkers; D019208:Brain-Derived Neurotrophic Factor; D005904:Glial Fibrillary Acidic Protein; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; D018696:Neuroprotective Agents; D016877:Oxidants; D011509:Proteoglycans; D000099216:Chondroitin Sulfate Proteoglycan 4; C528900:glial fibrillary astrocytic protein, mouse; C061195:neurofilament protein M; C076944:salvianolic acid B; D053630:Depsides