title: Epstein-Barr virus DNA associated with gastric adenocarcinoma and adjacent non-cancerous mucosa in patients from Manaus, Brazil.
abstract: Gastric  cancer is one of most frequent causes of death in Brazil. The city of Manaus  has one of the highest incidences of this disease in Brazil. The Epstein-Barr  virus (EBV) is a ubiquitous herpesvirus that is classified as a group 1  carcinogen by the International Agency for Research on Cancer. We obtained  biopsies from 6 control subjects and 10 patients with gastric carcinomas living  in Manaus. In the patients, the samples were taken from tumors and from  adjacent non-cancerous mucosa. These samples were screened for EBV DNA by PCR  to amplify the 288-bp fragments from the Bam M region. The EBV DNA was detected  in 8 of the 10 tumor cases and in none of the 6 control subjects. In the  positively identified samples, EBV DNA was detected in five corresponding  resection margins. Previous research indicated only a weak association between  EBV and gastric cancer. We suggest that EBV should be considered as a risk factor  for gastric adenocarcinomas in Manaus.
authors.affiliation: Departamento de Química, Universidade Federal do Amazonas, Manaus, AM, Brasil.
authors.forename: P F
authors.initials: PF
authors.lastname: de Aquino
authors.forename: P C
authors.initials: PC
authors.lastname: Carvalho
authors.forename: J S
authors.initials: JS
authors.lastname: da Gama Fischer
authors.forename: A Q L
authors.initials: AQ
authors.lastname: de Souza
authors.forename: J S
authors.initials: JS
authors.lastname: Viana
authors.forename: S R S
authors.initials: SR
authors.lastname: Chalub
authors.forename: A D L
authors.initials: AD
authors.lastname: de Souza
authors.forename: M G C
authors.initials: MG
authors.lastname: Carvalho
mesh_terms: D000230:Adenocarcinoma / Q000821:virology*; D000328:Adult; D000368:Aged; D001938:Brazil; D016022:Case-Control Studies; D004279:DNA, Viral / Q000235:genetics*; D020031:Epstein-Barr Virus Infections / Q000150:complications* / Q000821:virology; D005260:Female; D005753:Gastric Mucosa / Q000473:pathology / Q000821:virology*; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000821:virology*
chemical_list: D004279:DNA, Viral