title: Differential localization of LGR5 and Nanog in clusters of colon cancer stem cells.
abstract: One paradigm of cancer development claims that cancer emerges at the niche of tissue stem cells and these cells continue to proliferate in the tumor as cancer stem cells. LGR5, a membrane receptor, was recently found to be a marker of normal colon stem cells in colon polyps and is also expressed in colon cancer stem cells. Nanog, an embryonic stem cell nuclear factor, is expressed in several embryonic tissues, but Nanog expression is not well documented in cancerous stem cells. Our aim was to examine whether both LGR5 and Nanog are expressed in the same clusters of colon stem cells or cancer stem cells, using immunocytochemistry with specific antibodies to each antigen. We analyzed this aspect using paraffin embedded tumor tissue sections obtained from 18 polyps and 36 colon cancer specimens at stages I-IV. Antibodies to LGR5 revealed membrane and cytoplasm immunostaining of scattered labeled cells in normal crypts, with no labeling of Nanog. However, in close proximity to the tumors, staining to LGR5 was much more intensive in the crypts, including that of the epithelial cells. In cancer tissue, positive LGR5 clusters of stem cells were observed mainly in poorly differentiated tumors and in only a few scattered cells in the highly differentiated tumors. In contrast, antibodies to Nanog mainly stained the growing edges of carcinoma cells, leaving the poorly differentiated tumor cells unlabeled, including the clustered stem cells that could be detected even by direct morphological examination. In polyp tissues, scattered labeled cells were immunostained with antibodies to Nanog and to a much lesser extent with antibodies to LGR5. We conclude that expression of LGR5 is probably specific to stem cells of poorly differentiated tumors, whereas Nanog is mainly expressed at the edges of highly differentiated tumors. However, some of the cell layers adjacent to the carcinoma cell layers that still remained undifferentiated, expressed mainly Nanog with only a few cells labeled with antibodies to LGR5. Considering the different sites and pattern of expression in the tumor, our data imply that targeting the clustered stem cells expressing LGR5 in poorly differentiated colon cancer may require different strategies than targeting the stem cells expressing Nanog in the highly differentiated tumors. Alternatively, combined application of specific inhibitory miRNAs to Nanog and to LGR5 expression may assist therapeutically.
authors.affiliation: Department of Molecular Cell Biology, The Weizmann Institute of Science, 234, Herzl Street, Rehovot 76100, Israel. abraham.amsterdam@weizmann.ac.il
authors.forename: Abraham
authors.initials: A
authors.lastname: Amsterdam
authors.forename: Calanit
authors.initials: C
authors.lastname: Raanan
authors.forename: Letizia
authors.initials: L
authors.lastname: Schreiber
authors.forename: Ora
authors.initials: O
authors.lastname: Freyhan
authors.forename: Yakov
authors.initials: Y
authors.lastname: Fabrikant
authors.forename: Ehud
authors.initials: E
authors.lastname: Melzer
authors.forename: David
authors.initials: D
authors.lastname: Givol
mesh_terms: D002454:Cell Differentiation; D003106:Colon / Q000166:cytology / Q000473:pathology; D003110:Colonic Neoplasms / Q000473:pathology* / Q000503:physiopathology; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D000071317:Nanog Homeobox Protein; D060787:Neoplasm Grading; D014411:Neoplastic Stem Cells / Q000473:pathology*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*
chemical_list: D018398:Homeodomain Proteins; C116884:LGR5 protein, human; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D043562:Receptors, G-Protein-Coupled