title: Dehalogenation of 4 - Chlorobenzoic Acid by Pseudomonas isolates.
abstract: Twenty three bacterial isolates either pure or consortium were initially screened on the basis of their ability to degrade as well as dechlorinate 4 - chlorobenzoic acid (4-CBA). Based on comparative growth response, three pure isolates Pseudomonas putida GVS-4, Pseudomonas aeruginosa GVS-18 and Pseudomonas aeruginosa GWS-19 and a consortium SW-2 was finally selected for further studies. The enzyme studies performed with cell free extracts revealed that dehalogenase activity was substrate specific with maximum activity at 300 μgml(-1) substrate concentration. Catechol 1,2 dioxygenase activity was found to be present in cell free extracts suggesting that 4 - chlorobenzoic acid (4-CBA) is catabolized by ortho-ring cleavage pathway. The dehalogenase enzyme profile showed single enzyme band in case of GVS-4 (Rm 0.76), GVS-18 (Rm 0.84), GWS -19 (Rm 0.85) and two bands in SW-2 (Rm 0.71 & 0.10).
authors.affiliation: Department of Microbiology, Punjab Agricultural University, Ludhiana, 141 004 India.
authors.forename: G
authors.initials: G
authors.lastname: Banta
authors.forename: R S
authors.initials: RS
authors.lastname: Kahlon
keywords: 4-chlorobenzoic acid; Degradation; Dehalogenase; Pseudomonas aeruginosa; Pseudomonas putida