title: Diffusion and interaction dynamics of individual membrane protein complexes confined in micropatterned polymer-supported membranes.
abstract: Micropatterned polymer-supported membranes (PSM) are established as a tool for confining the diffusion of transmembrane proteins for single molecule studies. To this end, a photochemical surface modification with hydrophobic tethers on a PEG polymer brush is implemented for capturing of lipid vesicles and subsequent fusion. Formation of contiguous membranes within micropatterns is confirmed by scanning force microscopy, fluorescence recovery after photobleaching (FRAP), and super-resolved single-molecule tracking and localization microscopy. Free diffusion of transmembrane proteins reconstituted into micropatterned PSM is demonstrated by FRAP and by single-molecule tracking. By exploiting the confinement of diffusion within micropatterned PSM, the diffusion and interaction dynamics of individual transmembrane receptors are quantitatively resolved.
authors.affiliation: Department of Biology, University of Osnabrück, Osnabrück, Germany.
authors.forename: Sharon
authors.initials: S
authors.lastname: Waichman
authors.forename: Friedrich
authors.initials: F
authors.lastname: Roder
authors.forename: Christian P
authors.initials: CP
authors.lastname: Richter
authors.forename: Oliver
authors.initials: O
authors.lastname: Birkholz
authors.forename: Jacob
authors.initials: J
authors.lastname: Piehler
mesh_terms: D036681:Fluorescence Recovery After Photobleaching; D008565:Membrane Proteins / Q000737:chemistry*; D008567:Membranes, Artificial; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding
chemical_list: D008565:Membrane Proteins; D008567:Membranes, Artificial; D011108:Polymers