title: DNA from oral bacteria by sodium hydroxide-paper method suitable for polymerase chain reaction.
abstract: In the oral cavity, we can find a complex mixture of microorganisms, commensals, and pathogens. The studies of normal oral microbiota, as well as the studies of much oral pathology (e.g., caries, periodontitis), involve the isolation and cultivation of these microorganisms and their molecular analysis. The aim of this study was to validate a quick, easy, efficient, and inexpensive DNA extraction method for the recovery of genomic DNA from gram-positive and gram-negative oral bacteria to be used in polymerase chain reaction amplification. This method worked great with all samples analyzed, providing an approach to extract DNA for different microorganisms.
authors.affiliation: Laboratory of Biochemistry and Oral Biology, Department of Basic and Communitary Sciences, Faculty of Dentistry, University of Chile, Santiago, Chile.
authors.forename: Claudia
authors.initials: C
authors.lastname: Lefimil
authors.forename: Carla
authors.initials: C
authors.lastname: Lozano
authors.forename: Irene
authors.initials: I
authors.lastname: Morales-Bozo
authors.forename: Anita
authors.initials: A
authors.lastname: Plaza
authors.forename: Cristian
authors.initials: C
authors.lastname: Maturana
authors.forename: Blanca
authors.initials: B
authors.lastname: Urzúa
mesh_terms: D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008297:Male; D054892:Metagenome / Q000235:genetics; D009055:Mouth / Q000382:microbiology*; D010209:Paper; D016133:Polymerase Chain Reaction / Q000379:methods*; D012972:Sodium Hydroxide / Q000737:chemistry*
chemical_list: D004269:DNA, Bacterial; D012972:Sodium Hydroxide