title: Immunoglobulin-like transcript receptors on human dermal CD14+ dendritic cells act as a CD8-antagonist to control cytotoxic T cell priming.
abstract: Human Langerhans cells (LCs) are highly efficient at priming cytolytic CD8(+) T cells compared with dermal CD14(+) dendritic cells (DCs). Here we show that dermal CD14(+) DCs instead prime a fraction of naïve CD8(+) T cells into cells sharing the properties of type 2 cytokine-secreting CD8(+) T cells (TC2). Differential expression of the CD8-antagonist receptors on dermal CD14(+) DCs, the Ig-like transcript (ILT) inhibitory receptors, explains the difference between the two types of DCs. Inhibition of CD8 function on LCs inhibited cytotoxic T lymphocytes (CTLs) and enhanced TC2 generation. In addition, blocking ILT2 or ILT4 on dermal CD14(+) DCs enhanced the generation of CTLs and inhibited TC2 cytokine production. Lastly, addition of soluble ILT2 and ILT4 receptors inhibited CTL priming by LCs. Thus, ILT receptor expression explains the polarization of CD8(+) T-cell responses by LCs vs. dermal CD14(+) DCs.
authors.affiliation: Baylor Institute for Immunology Research and Baylor Research Institute, Dallas, TX 75204, USA.
authors.forename: Jacques
authors.initials: J
authors.lastname: Banchereau
authors.forename: Sandra
authors.initials: S
authors.lastname: Zurawski
authors.forename: LuAnn
authors.initials: L
authors.lastname: Thompson-Snipes
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Blanck
authors.forename: Sandra
authors.initials: S
authors.lastname: Clayton
authors.forename: Adiel
authors.initials: A
authors.lastname: Munk
authors.forename: Yanying
authors.initials: Y
authors.lastname: Cao
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Wang
authors.forename: Sunaina
authors.initials: S
authors.lastname: Khandelwal
authors.forename: Jiancheng
authors.initials: J
authors.lastname: Hu
authors.forename: William H
authors.initials: WH
authors.lastname: McCoy
authors.forename: Karolina A
authors.initials: KA
authors.lastname: Palucka
authors.forename: Yoram
authors.initials: Y
authors.lastname: Reiter
authors.forename: Daved H
authors.initials: DH
authors.lastname: Fremont
authors.forename: Gerard
authors.initials: G
authors.lastname: Zurawski
authors.forename: Marco
authors.initials: M
authors.lastname: Colonna
authors.forename: Andrey S
authors.initials: AS
authors.lastname: Shaw
authors.forename: Eynav
authors.initials: E
authors.lastname: Klechevsky
mesh_terms: D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D020405:Dermis / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007801:Langerhans Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D000075362:Leukocyte Immunoglobulin-like Receptor B1; D018950:Lipopolysaccharide Receptors; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C105299:LILRB1 protein, human; C491341:LILRB2 protein, human; D000075362:Leukocyte Immunoglobulin-like Receptor B1; D018950:Lipopolysaccharide Receptors; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic