title: Hypoglycemic effects of cocoa (Theobroma cacao L.) autolysates.
abstract: Fat, alkaloid and polyphenol contents of two clones of cocoa (UIT1 and PBC 140) were removed and the remaining powder was autolyzed at pH 3.5 and 5.2. Based on the results, autolysates of UIT produced at pH 3.5 exhibited the highest ability to inhibit α-amylase activity. However, no α-glucosidase inhibition activity was observed under the conditions specified. Autolysates produced under pH 3.5 caused the highest amount of insulin secretion. In streptozotocin-diabetic rats, all cocoa autolysates significantly decreased blood glucose at 4h. To assure that the results from the assays were not due to the polyphenols of cocoa autolysates qualitative and quantitative tests were applied. According to their results cocoa autolysates were found to be free from polyphenols. Analysis of amino acid composition revealed that cocoa autolysates were abundant in hydrophobic amino acids. It can be suggested that besides other compounds of cocoa, its peptides and amino acids could contribute to its health benefits.
authors.affiliation: Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Bahareh
authors.initials: B
authors.lastname: Sarmadi
authors.forename: Farhana
authors.initials: F
authors.lastname: Aminuddin
authors.forename: Muhajir
authors.initials: M
authors.lastname: Hamid
authors.forename: Nazamid
authors.initials: N
authors.lastname: Saari
authors.forename: Azizah
authors.initials: A
authors.lastname: Abdul-Hamid
authors.forename: Amin
authors.initials: A
authors.lastname: Ismail
mesh_terms: D000596:Amino Acids / Q000032:analysis / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002099:Cacao / Q000737:chemistry*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism; D006801:Humans; D007004:Hypoglycemic Agents / Q000008:administration & dosage* / Q000032:analysis; D007328:Insulin / Q000378:metabolism; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage* / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000596:Amino Acids; D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; D010936:Plant Extracts