title: Simultaneous estimation and validation of some binary mixtures of antihypertensive drugs by RP-LC methods using two new generation silica columns.
abstract: Two reversed phase liquid chromatographic (RP-LC) techniques are presented for the rapid, accurate, precise, simultaneous determination of olmesartan-hydrochlorothiazide and zofenopril-hydrochlorothiazide binary mixtures in their dosage forms. The separation of these binary mixtures was carried out by using two new stationary phases that have different surface chemistries which were used for the first time in the determination of these binary mixtures. The analyte peaks were detected at 216 nm. Linearity was obtained in different concentration ranges between 0.5 and 20 μg mL(-1) for all compounds. The proposed methods have been extensively validated and sample preparation, flow rate, run time of the analytical systems were at low levels. The proposed methods would decrease the consumption of organic solvents and reagents further safeguarding to our environment.
authors.affiliation: Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, Ankara, Turkey.
authors.forename: Sevinc
authors.initials: S
authors.lastname: Kurbanoglu
authors.forename: Mehmet
authors.initials: M
authors.lastname: Gumustas
authors.forename: Sibel A
authors.initials: SA
authors.lastname: Ozkan
mesh_terms: D000959:Antihypertensive Agents / Q000032:analysis*; D002216:Captopril / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D006852:Hydrochlorothiazide / Q000032:analysis; D007093:Imidazoles / Q000032:analysis; D007202:Indicators and Reagents / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013777:Tetrazoles / Q000032:analysis
chemical_list: D000959:Antihypertensive Agents; D007093:Imidazoles; D007202:Indicators and Reagents; D012997:Solvents; D013777:Tetrazoles; D006852:Hydrochlorothiazide; C044958:zofenopril; D012822:Silicon Dioxide; C437965:olmesartan; D002216:Captopril