title: Aloe-emodin inhibits proliferation of adult human keratinocytes in vitro.
abstract: Aloe-emodin (AE) is a plant-derived hydroxyanthraquinone with several biological activities. It is present in a variety of skin-conditioning agents containing aloe extracts, but its influence on keratinocyte growth was not examined so far. We investigated the influence of AE on human keratinocyte proliferation and apoptosis in vitro. AE significantly inhibited proliferation of cultivated human keratinocytes at 5 μM concentration, as revealed by incorporation of radioactive thymidine. The antiproliferative effect of AE was accompanied with induction of apoptosis, but not necrosis, as demonstrated by flow cytometric analysis and lactate dehydrogenase release assay. Based on the half maximal inhibitory concentration values, we demonstrated that AE may impair proliferation of keratinocytes at concentrations far below the industry standards for commercial products containing aloe extracts. Therefore, further research of AE effects on the human skin and proper labeling of products are necessary for maximizing benefits from aloe extracts and to avoid undesired responses.
authors.affiliation: Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: Dusan
authors.initials: D
authors.lastname: Popadic
authors.forename: Emina
authors.initials: E
authors.lastname: Savic
authors.forename: Zorica
authors.initials: Z
authors.lastname: Ramic
authors.forename: Vladimir
authors.initials: V
authors.lastname: Djordjevic
authors.forename: Vladimir
authors.initials: V
authors.lastname: Trajkovic
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Medenica
authors.forename: Svetlana
authors.initials: S
authors.lastname: Popadic
mesh_terms: D000328:Adult; D000880:Anthraquinones / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects*
chemical_list: D000880:Anthraquinones; C518327:aloe emodin