title: Ano-genital human papillomavirus type 97 infection is detected in Canadian men but not women at risk or infected with the human immunodeficiency virus.
abstract: BACKGROUND
  Human papillomavirus type 97 (HPV97) DNA was detected in nearly 5% of anal samples collected from HIV-seropositive men living in Montreal, Canada. The rate of detection of HPV97 in the genital tract of Canadian women is unknown. Whether HPV97 is a local epidemic in HIV-seropositive men living in Montreal is also unknown. The prevalence of human papillomavirus type 97 (HPV97) was assessed in cervicovaginal cells from women living in Canada and in anal samples from HIV-seropositive men living in Toronto.
  
  
  RESULTS
  Cervicovaginal lavages collected from 904 women (678 HIV-seropositive, 226 HIV-seronegative) women living in Canada and anal cells collected from 123 HIV-seropositive men living in Toronto were tested for the presence of HPV97 with PCR. HPV97-positive samples were further tested by PCR-sequencing for molecular variant analysis to assess if all HPV97-positive men were infected with the same strain. All cervicovaginal samples were negative for HPV97. HPV97 was detected in anal samples from 6 HIV-seropositive men (4.9%, 95% confidence interval 2.0-10.5%), of whom five had high-grade and one had low-grade anal intraepithelial neoplasia, in addition to 2 to 8 HPV genital genotypes per sample. Four HPV97 variants were defined by four variation sites in the viral control region.
  
  
  CONCLUSIONS
  These findings indicate that HPV97 infects in the anal canal of HIV-seropositive men but is not detected in the genital tract of women.
authors.affiliation: Département de Pathologie, Centre Hospitalier de l'Université de Montréal, Montréal, Québec, Canada.
authors.forename: Marie-Eve
authors.initials: ME
authors.lastname: Landry
authors.forename: Irving E
authors.initials: IE
authors.lastname: Salit
authors.forename: Catherine
authors.initials: C
authors.lastname: Rodrigues-Coutlée
authors.forename: Deborah
authors.initials: D
authors.lastname: Money
authors.forename: Anu
authors.initials: A
authors.lastname: Rebbapragada
authors.forename: Jill
authors.initials: J
authors.lastname: Tinmouth
authors.forename: Catherine
authors.initials: C
authors.lastname: Hankins
authors.forename: Isabelle
authors.initials: I
authors.lastname: Gorska-Flipot
authors.forename: Jacques
authors.initials: J
authors.lastname: Archambault
authors.forename: Eduardo L
authors.initials: EL
authors.lastname: Franco
authors.forename: François
authors.initials: F
authors.lastname: Coutlée
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001003:Anal Canal / Q000821:virology; D002170:Canada / Q000453:epidemiology; D015331:Cohort Studies; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005838:Genotype; D006678:HIV / Q000302:isolation & purification*; D015658:HIV Infections / Q000150:complications* / Q000453:epidemiology / Q000821:virology; D018023:HIV Seronegativity; D006679:HIV Seropositivity; D018451:Homosexuality, Male; D006801:Humans; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000150:complications / Q000175:diagnosis* / Q000821:virology; D011110:Polymorphism, Genetic; D015995:Prevalence; D012306:Risk; D012737:Sex Factors; D014621:Vagina / Q000821:virology; D055815:Young Adult
chemical_list: D004279:DNA, Viral