title: High FLT3 expression and IL10 (G1082A) polymorphism in poor overall survival in calla acute lymphoblastic leukemia.
abstract: Patients with acute lymphoblastic leukemia presenting the immunophenotypic marker CD10+ (calla), usually has treatment profile good. The FLT3 molecular marker is listed as a prognostic factor, an important leukaemogenic marker in acute leukemias, also the polymorphism (G1082A) of the IL10 interleukin can to present pleiotropic effects in many diseases and could is associated to development of ALL. However, the FLT3 expression is variability among patients with calla-ALL. The aim of this study was to determine the FLT3 expression, to associate with the genotypes and allelic of G1082A (IL10) in 50 patients with calla-ALL and assess the overall survival at 98 months follow-up. The expression was assessed by quantitative real time PCR (RT-PCR), the G1082A polymorphism was identified by allele-specific PCR and for immunophenotypic classification was used specific markers of B lineage-calla. We observed that patients who died showed higher FLT3 expression (p = 0.005), worse survival (p = 0.0137) and the IL10G allele may favor the survival, because the IL10 GG and IL10 GA genotypes showed a low FLT3 expression (p < 0.05).
authors.affiliation: Pediatric Hematology Oncology Center (CEONHPE/UPE), Recife, PE, Brazil. dayvasconcelos@hotmail.com
authors.forename: Dayse Maria Vasconcelos
authors.initials: DM
authors.lastname: de Deus
authors.forename: Paulo Roberto Eleutério
authors.initials: PR
authors.lastname: de Souza
authors.forename: Maria Tereza Cartaxo
authors.initials: MT
authors.lastname: Muniz
mesh_terms: D000293:Adolescent; D014408:Biomarkers, Tumor / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005260:Female; D015870:Gene Expression; D056726:Genetic Association Studies; D006801:Humans; D007223:Infant; D016753:Interleukin-10 / Q000235:genetics*; D053208:Kaplan-Meier Estimate; D008297:Male; D015260:Neprilysin / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000201:enzymology* / Q000235:genetics* / Q000401:mortality; D051941:fms-Like Tyrosine Kinase 3 / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C508609:IL10 protein, human; D016753:Interleukin-10; C497970:FLT3 protein, human; D051941:fms-Like Tyrosine Kinase 3; D015260:Neprilysin