title: Single molecular recognition force spectroscopy study of a luteinizing hormone-releasing hormone analogue as a carcinoma target drug.
abstract: The luteinizing hormone-releasing hormone- Pseudomonas aeruginosa exotoxin 40 (LHRH-PE40), is a candidate target drug associated with elevated LHRH receptor (LHRH-R) expression in malignant tumor tissue. The capability of LHRH-PE40 to recognize LHRH-Rs on a living cell membrane was studied with single molecular recognition force spectroscopy (SMFS) based on atomic force microscopy (AFM). The recognition force of LHRH-PE40/LHRH-R was compared with that of LHRH/LHRH-R by dynamic force spectroscopy. Meanwhile, cell growth inhibition assay and fluorescence imaging were presented as complementary characterization. The results show that LHRH moiety keeps its capability to recognize LHRH-R specifically, which implies that recombinant protein LHRH-PE40 can be a promising target drug.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Guangmou
authors.initials: G
authors.lastname: Wu
authors.forename: Chunli
authors.initials: C
authors.lastname: Song
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Li
authors.forename: Haiyan
authors.initials: H
authors.lastname: Qiao
authors.forename: Ping
authors.initials: P
authors.lastname: Zhu
authors.forename: Peter
authors.initials: P
authors.lastname: Hinterdorfer
authors.forename: Bailin
authors.initials: B
authors.lastname: Zhang
authors.forename: Jilin
authors.initials: J
authors.lastname: Tang
mesh_terms: D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000627:therapeutic use; D006801:Humans; D018625:Microscopy, Atomic Force; D009369:Neoplasms / Q000188:drug therapy; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D007987:Gonadotropin-Releasing Hormone