title: Histone deacetylase inhibitor MC1293 induces latent HIV-1 reactivation by histone modification in vitro latency cell lines.
abstract: HIV-1 latency remains a major problem for the eradication of viruses in infected individuals. We evaluated the effect of MC1293 on the epigenetic change at HIV-1 LTR and the induction of the latent viruses in the latency Jurkat T cell line. We found MC1293 can activate HIV-1 gene expression, increase the acetylation level of H3 and H4 at the nuc-1 site of HIV-1 LTR. In addition, MC1293 can synergize with prostratin to activate the HIV-1 promoter, and has relatively lower toxicity compared to Trichostatin A (TSA). The results suggest that the acetylation of histone plays an important role in regulating HIV-1 LTR gene expression, and MC1293 is potential drug candidate for antilatency therapies.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, P.R. China.
authors.forename: Xiying
authors.initials: X
authors.lastname: Qu
authors.forename: Hao
authors.initials: H
authors.lastname: Ying
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Wang
authors.forename: Chuijin
authors.initials: C
authors.lastname: Kong
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.forename: Pengfei
authors.initials: P
authors.lastname: Wang
authors.forename: Huanzhang
authors.initials: H
authors.lastname: Zhu
mesh_terms: D000107:Acetylation / Q000187:drug effects; D002273:Carcinogens / Q000494:pharmacology; D002460:Cell Line; D004357:Drug Synergism; D057890:Epigenomics; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D016325:HIV Long Terminal Repeat / Q000187:drug effects*; D015497:HIV-1; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D011758:Pyrroles / Q000494:pharmacology*; D017735:Virus Latency / Q000187:drug effects*
chemical_list: D002273:Carcinogens; D056572:Histone Deacetylase Inhibitors; D006657:Histones; C582792:MC1293; D011758:Pyrroles