title: Diversity and symbiotic effectiveness of indigenous rhizobia-nodulating Adesmia bicolor in soils of Central Argentina.
abstract: Native perennial legume Adesmia bicolor reveals characteristics that are key to securing persistence under grazing. Literature on the diversity and symbiotic effectiveness of indigenous rhizobia-nodulating A. bicolor in central Argentina is limited. The purpose of this study was therefore to determine phenotypic and genotypic variability as well as biological N-fixation effectiveness in rhizobia isolated from A. bicolor nodules. To this end, repetitive genomic regions were analyzed using ERIC primers. In the greenhouse, plants were grown under a (i) N-fertilized treatment, (ii) N-free control treatment, and (iii) rhizobia inoculation treatment. Dry weight and N-content were analyzed. All isolates belonged to Rhizobium genus and showed high symbiotic effectiveness. The N-content/subterranean N-content ratio in aerial and subterranean parts of inoculated plants was higher than that observed in N-fertilized plants during the vegetative stage. Results from this study demonstrate that symbiosis between native rhizobial strains and A. bicolor is very effective.
authors.affiliation: Laboratorio de Morfología Vegetal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Ruta 36 km 601, 5800, Río Cuarto, Provincia de Córdoba, Argentina. lbianco@ayv.unrc.edu.ar
authors.forename: Luciana
authors.initials: L
authors.lastname: Bianco
authors.forename: Jorge
authors.initials: J
authors.lastname: Angelini
authors.forename: Adriana
authors.initials: A
authors.lastname: Fabra
authors.forename: Rosana
authors.initials: R
authors.lastname: Malpassi
mesh_terms: D001118:Argentina; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D007887:Fabaceae / Q000382:microbiology* / Q000502:physiology; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation; D016133:Polymerase Chain Reaction; D012231:Rhizobium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013559:Symbiosis
chemical_list: D004269:DNA, Bacterial