title: Genomic sequence of Heliothis virescens ascovirus 3g isolated from Spodoptera exigua.
abstract: Heliothis virescens ascovirus 3a (HvAV-3a), a member of the family Ascoviridae, has the highest diversity among ascovirus species that have been reported in Australia, Indonesia, China, and the United States. To understand the diversity and origin of this important ascovirus, the complete genome of the HvAV Indonesia strain (HvAV-3g), isolated from Spodoptera exigua, was determined to be 199,721 bp, with a G+C content of 45.9%. Therefore, HvAV-3g has the largest genome among the reported ascovirus genomes to date. There are 194 predicted open reading frames (ORFs) encoding proteins of 50 or more amino acid residues. In comparison to HvAV-3e reported from Australia, HvAV-3g has all the ORFs in HvAV-3e with 6 additional ORFs unique to HvAV-3g, including 1 peptidase C26 gene with the highest identity to Drosophila spp. and 2 gas vesicle protein U (GvpU) genes with identities to Bacillus megaterium. The five unique homologous regions (hrs) and 25 baculovirus repeat ORFs (bro) of HvAV-3g are highly variable.
authors.affiliation: Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan, People's Republic of China.
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Huang
authors.forename: Yun-Sheng
authors.initials: YS
authors.lastname: Wang
authors.forename: Xing
authors.initials: X
authors.lastname: Wang
authors.forename: Tyler A
authors.initials: TA
authors.lastname: Garretson
authors.forename: Liang-Ying
authors.initials: LY
authors.lastname: Dai
authors.forename: Chuan-Xi
authors.initials: CX
authors.lastname: Zhang
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Cheng
mesh_terms: D000818:Animals; D029223:Ascoviridae / Q000235:genetics*; D004279:DNA, Viral; D030541:Databases, Genetic; D005814:Genes, Viral; D014644:Genetic Variation; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017422:Sequence Analysis, DNA; D018411:Spodoptera / Q000821:virology*
chemical_list: D004279:DNA, Viral