title: Prediction of periodontopathic bacteria in dental plaque of periodontal healthy subjects by measurement of volatile sulfur compounds in mouth air.
abstract: OBJECTIVE
  The aim of this study was to determine whether measurements of volatile sulfur compounds (VSCs) are useful to predict colonization of periodontopathic bacteria. For this purpose, we assessed the relationships among distributions of 4 species of periodontopathic bacteria in tongue coating and dental plaque, oral conditions including VSC concentration in mouth air, and smoking habit of periodontal healthy young subjects.
  
  
  METHODS
  The subjects were 108 young adults (mean age, 23.5±2.56 years) without clinical periodontal pockets. Information regarding smoking habit was obtained by interview. After VSC concentration in mouth, air was measured with a portable sulfide monitor (Halimeter(®)), non-stimulated saliva flow and dental caries status were assessed, and tongue coating and dental plaque samples were collected from the subjects. The tongue coating samples were weighed to determine the amount. The colonization of Porphyromonas gingivalis, Tannerella forsythia, Prevotella intermedia, and Treponema denticola in both tongue coating and plaque samples was investigated using species-specific polymerase chain reaction assays.
  
  
  RESULTS
  Significant relationships were observed between the colonization of periodontopathic bacteria in tongue coating and plaque samples, especially that of P. gingivalis. VSC concentration showed the most significant association with colonization of P. gingivalis in both tongue coating and dental plaque. Logistic regression analysis demonstrated that the adjusted partial correlation coefficient [Exp(B)] values for VSC concentration with the colonization of P. gingivalis, P. intermedia, and T. denticola in dental plaque were 135, 35.4 and 10.4, respectively. In addition, smoking habit was also shown to be a significant variable in regression models [Exp(B)=6.19, 8.92 and 2.53, respectively]. Therefore, receiver operating characteristic analysis was performed to predict the colonization of periodontal bacteria in dental plaque in the subjects divided by smoking habit. Based on our results, we found cut-off values that indicated likelihood ratios (LR) within the efficient range for positive findings in both groups.
  
  
  CONCLUSIONS
  The present results demonstrated that measurement of VSC concentration in mouth air is a useful method to predict the presence of colonization of some periodontopathic bacteria in dental plaque.
authors.affiliation: Department of Developmental Oral Health Science, Division of Oral Health, Iwate Medical University School of Dentistry, Morioka, Japan. mkishi@iwate-med.ac.jp
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Kishi
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ohara-Nemoto
authors.forename: Masahiro
authors.initials: M
authors.lastname: Takahashi
authors.forename: Kayo
authors.initials: K
authors.lastname: Kishi
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Kimura
authors.forename: Fumie
authors.initials: F
authors.lastname: Aizawa
authors.forename: Masami
authors.initials: M
authors.lastname: Yonemitsu
mesh_terms: D000328:Adult; D000388:Air; D058491:Bacterial Load; D001439:Bacteroides / Q000302:isolation & purification; D004245:DMF Index; D003773:Dental Plaque / Q000382:microbiology*; D005260:Female; D005544:Forecasting; D006090:Gram-Negative Bacteria / Q000145:classification*; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis; D008297:Male; D010510:Periodontal Diseases / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000302:isolation & purification; D018713:Prevotella intermedia / Q000302:isolation & purification; D012372:ROC Curve; D012463:Saliva / Q000378:metabolism; D012680:Sensitivity and Specificity; D012907:Smoking / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000032:analysis; D013440:Sulfides / Q000032:analysis; D013457:Sulfur Compounds / Q000032:analysis*; D014059:Tongue / Q000382:microbiology; D048373:Treponema denticola / Q000302:isolation & purification; D055549:Volatile Organic Compounds / Q000032:analysis*; D055815:Young Adult
chemical_list: D013438:Sulfhydryl Compounds; D013440:Sulfides; D013457:Sulfur Compounds; D055549:Volatile Organic Compounds; C005231:methylmercaptan; C004784:dimethyl sulfide; D006862:Hydrogen Sulfide