title: Evaluation of anxiolytic effect of Erythrina mysorensis Gamb. in mice.
abstract: OBJECTIVE
  To evaluate anxiolytic effect of stem bark ethanol and chloroform extracts of Erythrina mysorensis in mice.
  
  
  METHODS
  The anxiolytic activity was examined by using the elevated plus maze (EPM) and open field test (OFT), and motor coordination by rotarod test (RRT). Twenty four Swiss albino male mice were divided into four groups of six mice each. Group 1 received vehicle (normal saline); group 2 received diazepam (1 mg/kg); groups 3 and 4 received ethanolic and chloroform extract of Erythrina mysorensis, 200 and 400 mg/kg p.o., respectively.
  
  
  RESULTS
  Mice treated with diazepam (1 mg/kg, p.o.) showed significant (P < 0.001) increase ini the percentage of open arms entries and time spent whereas, in closed arm the number of entries and time spent were significantly (P < 0.05) decreased. Oral administration of chloroform and ethanol extract of E. mysorensis exhibited significant (P < 0.05) increase in the number of open arm entries and time spent with significant (P < 0.05) reduction in number of entries and time spent in the closed arm as compared to group 1. Chloroform and ethanol extracts treated mice also produced significant increase in the number of rearings (P < 0.05), assisted rearings and number of squares crossed (P < 0.01). Rotarod test showed significant (P < 0.01) reduction in motor activity at 45 min with diazepam and E. mysorensis extracts (400 mg/kg) as compared to groups 3 and 1.
  
  
  CONCLUSIONS
  Erythrina mysorensis possess significant anxiolytic activity in the mice. It can be a promising anxiolytic agent.
authors.affiliation: Department of Pharmaceutics, SJM College of Pharmacy, Chitradurga, India.
authors.forename: Thandaga S
authors.initials: TS
authors.lastname: Nagaraja
authors.forename: Riaz
authors.initials: R
authors.lastname: Mahmood
authors.forename: V
authors.initials: V
authors.lastname: Krishna
authors.forename: B S
authors.initials: BS
authors.lastname: Thippeswamy
authors.forename: V P
authors.initials: VP
authors.lastname: Veerapur
mesh_terms: D000818:Animals; D014151:Anti-Anxiety Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D001007:Anxiety / Q000188:drug therapy* / Q000523:psychology; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004895:Erythrina; D008297:Male; D018782:Maze Learning / Q000187:drug effects / Q000502:physiology; D051379:Mice; D024301:Plant Bark; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D011897:Random Allocation
chemical_list: D014151:Anti-Anxiety Agents; D010936:Plant Extracts
keywords: Elevated plus maze test; Erythrina mysorensis; Rotarod test; open field test