title: Compensation for matrix effects in the gas chromatography-mass spectrometry analysis of 186 pesticides in tea matrices using analyte protectants.
abstract: A gas chromatography-mass spectrometry (GC-MS) analytical method was developed for simultaneously determining 186 pesticides in tea matrices using analyte protectants to counteract the matrix-induced effect. The matrix effects were evaluated for green, oolong and black tea, representing unfermented, partially fermented and completely fermented teas respectively and depending on the type of tea, 72%, 94% and 94% of the pesticides presented strong response enhancement effect. Several analyte protectants as well as certain combinations of these protectants were evaluated to check their compensation effects. A mixture of triglycerol and d-ribonic acid-γ-lactone (both at 2mg/mL in the injected samples) was found to be the most effective in improving the chromatographic behavior of the 186 pesticides. More than 96% of the 186 pesticides achieved recoveries within the range of 70-120% when using the selected mixture of analyte protectants. The simple addition of analyte protectants offers a more convenient solution to overcome matrix effects, results in less active sites compared to matrix-matched standardization and can be an effective approach to compensate for matrix effects in the GC-MS analysis of pesticide residues.
authors.affiliation: College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Chunlin
authors.initials: C
authors.lastname: Fan
authors.forename: Guofang
authors.initials: G
authors.lastname: Pang
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D005990:Glycerol / Q000737:chemistry; D007783:Lactones / Q000737:chemistry; D010573:Pesticide Residues / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D015203:Reproducibility of Results; D012266:Ribose / Q000737:chemistry; D013662:Tea / Q000737:chemistry*
chemical_list: D007783:Lactones; D010573:Pesticide Residues; D013662:Tea; D012266:Ribose; D005990:Glycerol