title: Molecular and immunological characterization of wheat Serpin (Tri a 33).
abstract: METHODS
  Several wheat proteins are responsible for food and respiratory allergies. Due to their large polymorphism, the allergenic potential of a number of them has not yet been precisely established. The aim of this work was to perform a thorough assessment of serpin (Tri a 33) allergenicity.
  
  
  RESULTS
  Recombinant wheat Serpin-Z2B isoform (rSerpin-Z2B) was expressed in Escherichia coli. Synchrotron radiation circular dichroism data indicated that the recombinant serpin contains slightly more β-strands than α-helix structures. IgE reactivity of sera from 103 patients with food allergy and 29 patients with Baker's asthma was evaluated using ELISA, a model of basophil activation and linear epitope mapping (Pepscan). Twenty percent of patients with food allergy to wheat and 31% of those with Baker's asthma displayed rSerpin-Z2B-specific IgE in ELISA. The protein was able to induce IgE-dependent basophil degranulation. The Pepscan experiment identified four regions involved in IgE binding to serpin. Heating the protein induced its irreversible denaturation and impaired IgE binding, revealing the predominance of conformational epitopes.
  
  
  CONCLUSIONS
  This study confirms wheat serpin allergenicity and shows that recombinant serpin may be a marker of a broad spectrum of sensitization to wheat proteins.
authors.affiliation: INRA, UR 1268 Biopolymères Interactions Assemblages, Nantes, France.
authors.forename: Hamza
authors.initials: H
authors.lastname: Mameri
authors.forename: Sandra
authors.initials: S
authors.lastname: Denery-Papini
authors.forename: Manon
authors.initials: M
authors.lastname: Pietri
authors.forename: Olivier
authors.initials: O
authors.lastname: Tranquet
authors.forename: Colette
authors.initials: C
authors.lastname: Larré
authors.forename: Martine
authors.initials: M
authors.lastname: Drouet
authors.forename: Evelyne
authors.initials: E
authors.lastname: Paty
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Jonathan
authors.forename: Etienne
authors.initials: E
authors.lastname: Beaudouin
authors.forename: Denise-Anne
authors.initials: DA
authors.lastname: Moneret-Vautrin
authors.forename: Thierry
authors.initials: T
authors.lastname: Moreau
authors.forename: Pierre
authors.initials: P
authors.lastname: Briozzo
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Gaudin
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000009:adverse effects / Q000276:immunology; D052179:Antigens, Plant / Q000276:immunology*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010940:Plant Proteins / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D015843:Serpins / Q000276:immunology*; D014908:Triticum / Q000737:chemistry* / Q000276:immunology; D021182:Wheat Hypersensitivity / Q000175:diagnosis / Q000276:immunology*; D055815:Young Adult
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D010940:Plant Proteins; D011994:Recombinant Proteins; D015843:Serpins; D007073:Immunoglobulin E