title: [Comparison of nucleic acid extraction efficiency using different commercial kits and qPCR. Effect of inhibitors].
abstract: The detection of specific nucleic acid (NA) sequences by PCR has revolutionized the biological and medical sciences. Real-time PCR (qPCR) opened up the possibility of obtaining quantitative results.  NA extraction is a decisive step prior to qPCR since it may produce either the removal or co-extraction of inhibitory substances of the enzymatic reaction, which in turn affects the amplification efficiency. In the present work we compared the commercial NA extraction kits from Qiagen, Invitrogen and Macherey-Nagel, which were used to extract DNA from mice blood artificially infected with Trypanosoma cruzi and PP7 RNA, Pseudomonas aeruginosa bacteriophage, in spiked aqueous matrices. NA recovery efficiency in samples without inhibitors was similar for the three extraction kits. However, the Invitrogen kit was the only one that remained unaffected in the presence of inhibitors in the samples.
authors.affiliation: Instituto de Investigaciones para la Industria Química - Consejo Nacional de Investigaciones Científicas y Técnicas (INIQUI - CONICET), Universidad Nacional de Salta (UNSa), Avenida Bolivia 5150, Salta (4400), Argentina.
authors.forename: Hugo R
authors.initials: HR
authors.lastname: Poma
authors.forename: Carolina
authors.initials: C
authors.lastname: Davies
authors.forename: Dolores
authors.initials: D
authors.lastname: Gutiérrez Cacciabue
authors.forename: María C
authors.initials: MC
authors.lastname: Mora
authors.forename: Miguel Á
authors.initials: MÁ
authors.lastname: Basombrío
authors.forename: Verónica B
authors.initials: VB
authors.lastname: Rajal
mesh_terms: D000818:Animals; D001769:Blood / Q000382:microbiology*; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification*; D006146:Guanidines / Q000494:pharmacology; D008297:Male; D051379:Mice; D009994:Osmolar Concentration; D017105:Pseudomonas Phages / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000821:virology*; D012317:RNA Phages / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology*; D013634:Tannins / Q000494:pharmacology*; D013861:Thiocyanates / Q000494:pharmacology; D014349:Trypanosoma cruzi / Q000235:genetics*; D014867:Water
chemical_list: D016054:DNA, Protozoan; D006146:Guanidines; D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic; D018894:Reverse Transcriptase Inhibitors; D013634:Tannins; D013861:Thiocyanates; D014867:Water; C054436:guanidine thiocyanate