title: Cholinesterase inhibitory constituents from Ficus bengalensis.
abstract: Bengalensinone (22β-hydroxylup-12,20-dien-3-one; 1), a new lupane triterpene, and benganoic acid (2), a new apocarotenoid, together with lupanyl acetate, 3-acetoxy-9(11),12-ursandiene, stigmasterol, alpinumisoflavone, 4-hydroxyacetophenone, 4-hydroxybenzoic acid, 4-hydroxymellein, and p-coumeric acid were isolated from the methanolic extract of the aerial roots of Ficus bengalensis. The structures of these compounds were established by the combination of 1D NMR (¹H and ¹³C NMR) and 2D NMR (HMQC, HMBC, and COSY) analyses, and mass spectrometry (EI-MS and HR-EI-MS), and in comparison with literature data of the related compounds. Compounds 1 and 2 displayed inhibitory potential against enzyme cholinesterase in a concentration-dependent manner with IC₅₀ values 194.5 and 154.5 μM for acetylcholinesterase and 224.9 and 120.0 μM for butyrycholinesterase, respectively.
authors.affiliation: Department of Chemistry, Baghdad-ul-Jadeed Campus, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan. nrch322@yahoo.com
authors.forename: Naheed
authors.initials: N
authors.lastname: Riaz
authors.forename: Muhammad Akram
authors.initials: MA
authors.lastname: Naveed
authors.forename: Muhammad
authors.initials: M
authors.lastname: Saleem
authors.forename: Bushra
authors.initials: B
authors.lastname: Jabeen
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ashraf
authors.forename: Syeda Abida
authors.initials: SA
authors.lastname: Ejaz
authors.forename: Abdul
authors.initials: A
authors.lastname: Jabbar
authors.forename: Ishtiaq
authors.initials: I
authors.lastname: Ahmed
mesh_terms: D000098:Acetophenones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D030681:Ficus / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010154:Pakistan; D018517:Plant Roots / Q000737:chemistry; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D053978:Pentacyclic Triterpenes
chemical_list: D000098:Acetophenones; D002800:Cholinesterase Inhibitors; D014315:Triterpenes; C578214:bengalensinone; C480546:lupane; D053978:Pentacyclic Triterpenes; C031335:4-hydroxyacetophenone