title: Antibodies enhance the infection of phorbol-ester-differentiated human monocyte-like cells with coxsackievirus B4.
abstract: Coxsackievirus B4 (CV-B4), in presence of antibodies and through a specific viral receptor CAR and Fcγ receptors II and III, can infect monocytes which results in interferon-α synthesis. The antibody-dependent enhancement of CV-B4 infection in the human monocytic-like THP-1 cell line has been investigated. The preincubation of CV-B4 with human plasma or human polyvalent immunoglobulins enhanced the infection of phorbol-myristate-acetate (PMA)-activated THP-1 cell cultures. CV-B4 replicated in these cells as demonstrated by the intracellular detection of infectious particles, viral protein VP1 (immunofluorescence), positive and negative viral RNA (RT-PCR). The viability of infected and control cell cultures was not different up to 20 days post-infection. Activated cell cultures inoculated with CV-B4 harbored intracellular RNA up to 14 days post-infection and produced IFNα that was detected by intracellular immunofluorescence staining as soon as 4 h post-infection with a maximum at 48 h post-infection and by RT-PCR all along the experiment. Together, these data demonstrate that PMA-activated THP-1 cells can be infected with CV-B4, can produce IFNα as a result of interactions between the virus, antibodies and specific receptors. This cellular model can be used to investigate further the mechanism and the result of the antibody-dependent enhancement of CV-B4 infection.
authors.affiliation: Université Lille 2, CHRU Lille, Laboratoire de Virologie/EA3610, Institut Hippocrate CHRU, 152 Rue du Dr Yersin, 59120 Loos-lez-Lille, France.
authors.forename: A
authors.initials: A
authors.lastname: Goffard
authors.forename: E K
authors.initials: EK
authors.lastname: Alidjinou
authors.forename: F
authors.initials: F
authors.lastname: Sané
authors.forename: L
authors.initials: L
authors.lastname: Choteau
authors.forename: C
authors.initials: C
authors.lastname: Bouquillon
authors.forename: D
authors.initials: D
authors.lastname: Caloone
authors.forename: P E
authors.initials: PE
authors.lastname: Lobert
authors.forename: D
authors.initials: D
authors.lastname: Hober
mesh_terms: D002478:Cells, Cultured; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein / Q000276:immunology; D003384:Coxsackievirus Infections / Q000276:immunology / Q000821:virology; D029822:Enterovirus B, Human / Q000276:immunology* / Q000472:pathogenicity; D054884:Host-Pathogen Interactions / Q000187:drug effects; D006801:Humans; D007136:Immunoglobulins / Q000494:pharmacology*; D016898:Interferon-alpha / Q000096:biosynthesis / Q000276:immunology; D042541:Intracellular Space / Q000378:metabolism / Q000821:virology; D009000:Monocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism / Q000821:virology*; D017452:Receptors, IgG / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D007136:Immunoglobulins; D016898:Interferon-alpha; D017452:Receptors, IgG; C016658:phorbolol myristate acetate; D013755:Tetradecanoylphorbol Acetate