title: Urinary 8-OHdG: A marker of oxidative stress to DNA and total antioxidant status in essential hypertension with South Indian population.
abstract: Establishment of non-invasive urinary biomarker for the early prediction of essential hypertension (EH) is important. We evaluated whether estimation of urinary DNA, serves as a marker to predict the extent of cellular oxidative stress in essential hypertension. A total of 180 South Indian subjects aged 30-65 were recruited for the study. Of these hypertensive subjects investigated, 30 were newly diagnosed and were not on any antihypertensive drugs, but had systolic blood pressure 140-160 mmHg and diastolic blood pressure 95-100 mmHg and 75 hypertensive patients who already on drug therapy for one year and 75 were South Indian normotensive healthy controls with blood pressure ≤ 120/80 mmHg. The 8-OHdG level in urine was significantly increased in hypertensive patients (both newly diagnosed and who already on drug therapy) compared with control group. The significant increase in 8-OHdG was observed in newly diagnosed hypertensive patients compared with hypertensive patients who already on drug therapy. There was a significant decrease in serum TAS value in essential hypertensive group when compared to control group. The urinary 8-OHdG was independently correlated with serum TAS. Decreased TAS levels, which reflect to increased oxidative stress, may be the reason of increased urinary 8-OHdG in South Indian hypertensive patients. Our preliminary data suggest that the competitive ELISA for 8-OHdG appears to be a simple method for quantifying the extent of oxidative stress.
authors.affiliation: Department of Biochemistry, Dr K.M Cherian Heart Foundation (A unit of Frontier Life Line Pvt Ltd), Mogappair, Chennai, 101 India.
authors.forename: P
authors.initials: P
authors.lastname: Subash
authors.forename: Prema
authors.initials: P
authors.lastname: Gurumurthy
authors.forename: A
authors.initials: A
authors.lastname: Sarasabharathi
authors.forename: K M
authors.initials: KM
authors.lastname: Cherian
keywords: Essential Hypertension; Oxidative Stress; Total Antioxidant Status; Urinary 8-OHdG