title: Biolistics-based gene silencing in plants using a modified particle inflow gun.
abstract: RNA interference (RNAi) is one of the most commonly used techniques for examining the function of genes of interest. In this chapter we present two examples of RNAi that use the particle inflow gun for delivery of the DNA constructs. In one example transient RNAi is used to show the function of an anthocyanin regulatory gene in flower petals. In the second example stably transformed cell cultures are produced with an RNAi construct that results in a change in the anthocyanin hydroxylation pattern.
authors.affiliation: The New Zealand Institute for Plant and Food Research Limited, Palmerston North, New Zealand. Kevin.Davies@plantandfood.co.nz
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Davies
authors.forename: Simon C
authors.initials: SC
authors.lastname: Deroles
authors.forename: Murray R
authors.initials: MR
authors.lastname: Boase
authors.forename: Don A
authors.initials: DA
authors.lastname: Hunter
authors.forename: Kathy E
authors.initials: KE
authors.lastname: Schwinn
mesh_terms: D032161:Antirrhinum / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D019470:Biolistics / Q000295:instrumentation*; D002478:Cells, Cultured; D046508:Culture Techniques; D003577:Cytochrome P-450 Enzyme System / Q000172:deficiency / Q000235:genetics; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics; D035264:Flowers / Q000254:growth & development; D006046:Gold / Q000737:chemistry; D055029:Inverted Repeat Sequences / Q000235:genetics; D010641:Phenotype; D010858:Pigmentation / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D034622:RNA Interference; D011198:Solanum tuberosum / Q000166:cytology / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors; D006046:Gold; D004247:DNA; D003577:Cytochrome P-450 Enzyme System; C084258:flavonoid 3',5'-hydroxylase