title: Characterization of activin A in the culture of primitive human umbilical cord blood hematopoietic cells.
abstract: Transforming growth factor-β (TGF-β) superfamily controls many physiological processes such as cell proliferation and differentiation, immune responses, wound repair and various endocrine activities. As a member of TGF-β, activin A can maintain the pluripotency of embryonic stem cells. We report here that activin A exhibited cell type-dependent function of expanding the human primitive hematopoietic cells isolated from umbilical cord blood (UCB). However, the multipotency of the cells pretreated with activin A was exhausted in the sequential dilution culture. In conclusion, activin A may not be a key factor, but a regulator, in the multipotency maintenance of primitive hematopoietic cells and the application of activin A in the hematopoietic stem/progenitor cells (HS/PCs) culture expansion remains a significant challenge.
authors.affiliation: Laboratory of Regeneromics, School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhao
authors.forename: Jin
authors.initials: J
authors.lastname: Gao
authors.forename: Shunying
authors.initials: S
authors.lastname: Zhu
authors.forename: Lan
authors.initials: L
authors.lastname: Qian
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Yu
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.forename: Mingyuan
authors.initials: M
authors.lastname: Wu
mesh_terms: D028341:Activins / Q000494:pharmacology* / Q000502:physiology; D018952:Antigens, CD34 / Q000096:biosynthesis; D039481:CD11b Antigen / Q000096:biosynthesis; D002452:Cell Count; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis
chemical_list: D018952:Antigens, CD34; D039481:CD11b Antigen; C509295:ITGAM protein, human; D018950:Lipopolysaccharide Receptors; C422405:activin A; D028341:Activins