title: Formation of interspecies fusants of Agaricus bisporus and Agaricus bitorquis mushroom by protoplast fusion.
abstract: Interspecies fusants are formed between Agaricus bisporus and Agaricus bitorquis by protoplast fusion technique. Protoplasts were isolated and regenerated by using Novozyme 234 lytic enzyme. Twenty slow growing isolates were separated from the protoplast regenerated colonies, which were assumed as homokaryons (putative homokaryons). These twenty isolates were subjected to growth rate, colony morphology and spawn run studies for screening of true homokaryons. Antifungal markers were developed for selection of fusants.
authors.affiliation: Fungal Biotechnology and Invertebrate Pathology Laboratory, Department of Biological Sciences, R.D. University, Jabalpur, India.
authors.forename: Rana Inder
authors.initials: RI
authors.lastname: Singh
authors.forename: Kanojiya
authors.initials: K
authors.lastname: Aarti
authors.forename: Sandhu Sardul
authors.initials: SS
authors.lastname: Singh
keywords: Agaricus bisporus; Antifungal markers; Fusants; Homokaryons; Protoplast fusion