title: Induction of adipogenic differentiation in three-dimensional culture model on a novel microfabricated scaffold.
abstract: The development of a three-dimensional (3D) culture system is very important for regenerative medicine and drug discovery applications of stem cell technology because the 3D culture condition could mimic the stem cell environment in vivo and support accurate differentiation. This chapter describes a novel 3D culture technique for efficient induction of adipogenic differentiation. This 3D culture system provides an easy way to allow cells to form a 3D spheroid structure without any matrix derived from animal and chemical substances. We firstly describe the details of the 3D culture technique using human mesenchymal stem/progenitor cells (MPCs) and its optimization. Then we elaborate on the protocol of efficient induction for adipogenic differentiation and adipocyte-specific gene expression, including peroxisome proliferator-activated receptor-γ, with our culture system in human MPCs.
authors.affiliation: Department of Pediatric Hematology and Oncology Research, National Research Institute for Child Health and Development, Tokyo, Japan. miyayoshi0007@gmail.com
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Miyagawa
authors.forename: Hajime
authors.initials: H
authors.lastname: Okita
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Kiyokawa
mesh_terms: D050156:Adipogenesis; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques / Q000295:instrumentation*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology; D055616:Microtechnology / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation*; D047495:PPAR gamma / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D014280:Triglycerides / Q000378:metabolism
chemical_list: D047495:PPAR gamma; D014280:Triglycerides; D012313:RNA