title: Effect of storage time on haematological parameters in mullet, Mugil cephalus.
abstract: The aim of the present study was to assess the effect of storage time at +4 °C on red blood cell count (RBC), haematocrit (Hct), haemoglobin (Hb), white blood cell count (WBC), thrombocyte count (TC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) in mullet (Mugil cephalus) using an automatic method. After blood collection (T0 ), all samples were analyzed using both the manual and automatic method. To test the validation of the automatic method, a paired t-test was applied, and no statistical difference was observed. The samples were successively divided into four different aliquots and stored at +4 °C to assess the haematological parameters using the automatic method. The first aliquot was refrigerated for 6 h, the second one for 24 h, the third one for 48 h and the last one at for 72 h. One-way repeated-measures ANOVA showed a significant effect of storage time (P < 0.05) on Hb, WBC, TC, MCH and MCHC. These results suggest that haematological parameters can be assessed within 6 h from blood collection when samples are stored at +4 °C because long-term storage modifies the results of the analyses. Further studies on these parameters could be still needed in various fish species to validate an appropriate method for haematological analysis useful not only for the evaluation of the health status of animal living in captivity and in aquaculture but also to have reliability environmental haematological biomarkers.
authors.affiliation: Faculty of Science, Department of Life Sciences M. Malpighi Section of General Physiology, University of Messina, Viale Ferdinando Stagno d'Alcontres, 31 S. Agata, 98166 Messina, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Faggio
authors.forename: S
authors.initials: S
authors.lastname: Casella
authors.forename: F
authors.initials: F
authors.lastname: Arfuso
authors.forename: S
authors.initials: S
authors.lastname: Marafioti
authors.forename: G
authors.initials: G
authors.lastname: Piccione
authors.forename: F
authors.initials: F
authors.lastname: Fazio
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D057205:Automation, Laboratory; D001792:Blood Platelets / Q000737:chemistry* / Q000166:cytology; D004906:Erythrocyte Count; D004909:Erythrocyte Indices; D004912:Erythrocytes / Q000737:chemistry* / Q000166:cytology; D006400:Hematocrit; D007958:Leukocyte Count; D007962:Leukocytes / Q000737:chemistry* / Q000166:cytology; D008297:Male; D010976:Platelet Count; D012034:Refrigeration / Q000592:standards*; D015203:Reproducibility of Results; D023701:Smegmamorpha / Q000097:blood*; D013048:Specimen Handling / Q000592:standards*
keywords: Mugil cephalus; haematological automatic analysis; haematological parameters; storage time; teleost