title: Larva Migration and Eosinophilia in Mice Experimentally Infected With Gnathostoma spinigerum.
abstract: BACKGROUND
  Gnathostoma spinigerum causes larva migran in human which is endemic in Southeast Asia. Information regarding larva migration is limited. In this study, we investigated the parasite migration by recovery of worms from the whole body of mouse after oral infection with advanced third stage larvae (AL3). The percentage of blood eosinophils was examined in parallel.
  
  
  METHODS
  Mice were orally infected with AL3 and histological study of organs was investigated in order to study the migration of AL3, along with blood eosinophilia.
  
  
  RESULTS
  At 1 hr post infection (PI), the larvae remained in the stomach, thereafter at 3, 5, 7, 10 and 24 hr PI; they were recovered from various organs including liver, mesentery, esophagus, diaphragm, lung, heart and dorsal fat. At day 15 PI, they were mostly found in muscles (76.47%). The average worm recovery (5 months) was 78.03%. The worms were found in the liver at every time point. Larva encystment was detected. There was a significant difference in blood eosinophils between the 8 larvae- (average 9.33% + 6.25%) and the 15 larvae-infected groups (average 22.66% + 11.03%). Surprisingly, the blood eosinophils (average 19.00% + 2.92%) were not higher in the higher infective dose- group (25 larvae).
  
  
  CONCLUSIONS
  Liver was involved by G. spinigerum throughout the study. We detected larva encystment which had never been reported in human gnathostomiasis. The highest percentage of eosinophil occurred during the invasive stage.
authors.affiliation: Dept. of Microbiology, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand ; Dept. of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
authors.forename: W
authors.initials: W
authors.lastname: Saksirisampant
authors.forename: N
authors.initials: N
authors.lastname: Choomchuay
authors.forename: K
authors.initials: K
authors.lastname: Kraivichian
authors.forename: B Wongsatayanon
authors.initials: BW
authors.lastname: Thanomsub
keywords: Eosinophil; Gnathostoma spinigerum; Larva migration; Larvae; Worm