title: Development of microsatellite markers from Mussaenda pubescens (Rubiaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed and characterized in Mussaenda pubescens for further study of its levels of genetic diversity and changes in population genetic structure in reproductive character displacement and in shifts of sexual systems.
  
  
  RESULTS
  Nineteen microsatellite loci were amplified successfully in M. pubescens, 17 of which were polymorphic. A maximum of eight alleles were detected per locus in 68 individuals at population level. The observed and expected heterozygosities varied from 0 to 1.000 and 0 to 0.882, respectively.
  
  
  CONCLUSIONS
  These newly developed microsatellite markers will be useful in further investigations of genetic diversity and gene flow among populations of M. pubescens and its congeneric species.
authors.affiliation: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, People's Republic of China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Duan
authors.forename: Wei
authors.initials: W
authors.lastname: Gong
authors.forename: Dian-Xiang
authors.initials: DX
authors.lastname: Zhang
mesh_terms: D000483:Alleles; D002681:China; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D051456:Gene Flow; D014644:Genetic Variation; D005838:Genotype; D005843:Geography; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D019666:Rubiaceae / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018744:DNA, Plant