title: Protoplast formation and regeneration in Lactobacillus delbrueckii.
abstract: Method for production and regeneration of Lactobacillus delbrueckii protoplasts are described. The protoplasts were obtained by treatment with a mixture of lysozyme and mutanolysin in protoplast buffer at pH 6.5 with different osmotic stabilizers. The protoplasts were regenerated on deMan, Rogosa and Sharpe (MRS) with various osmotic stabilizers. Maximum protoplast formation was obtained in protoplast buffer with sucrose as an osmotic stabilizer using a combination of lysozyme (1 mg/ml) and mutanolysin (10 μg/ml). Maximum protoplast regeneration was obtained on MRS medium with sucrose (0.5 M) as an osmotic stabilizer. The regeneration medium was also applicable to other species of lactobacilli as well. This is, to our knowledge, the first report on protoplast formation and efficient regeneration in case of L. delbrueckii.
authors.affiliation: National Chemical Laboratory, NCIM Resource Center, Pune, 411 008 Maharashtra India.
authors.forename: Mamta
authors.initials: M
authors.lastname: Singhvi
authors.forename: Dipti
authors.initials: D
authors.lastname: Joshi
authors.forename: Shalaka
authors.initials: S
authors.lastname: Gaikaiwari
authors.forename: Digambar V
authors.initials: DV
authors.lastname: Gokhale
keywords: Lactobacillus delbrueckii; Protoplast formation; Protoplast regeneration