title: Enhancement of biological mass spectrometry by using separations based on changes in ion mobility (FAIMS and DMS).
abstract: Analysis of complex biological samples for low-level analytes by liquid chromatography-tandem mass spectrometry (LC-MS/MS) often requires additional selectivity. Differential mobility techniques (FAIMS and DMS) have been shown to enhance LC-MS/MS analyses by separating ions in the gas-phase on a millisecond timescale by use of a mechanism that is complementary to both liquid chromatography and mass spectrometry. In this overview, a simplified description of the operation of these devices is given and an example presented that illustrates the utility of FAIMS (DMS) for solving a challenging analytical assay. Important recent advances in the field, including work with gas modifiers, are presented, along with an outlook for the technology.
authors.affiliation: National Research Council of Canada, 110 Gymnasium Place, Saskatoon, SK S7N 0W9, Canada. randy.purves@nrc-cnrc.gc.ca
authors.forename: Randy W
authors.initials: RW
authors.lastname: Purves
mesh_terms: D000818:Animals; D002623:Chemistry Techniques, Analytical; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D007477:Ions / Q000737:chemistry*; D008956:Models, Chemical; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors
chemical_list: D007477:Ions