title: Associations among serum N-terminal procollagen type III concentration, urinary aldosterone-to-creatinine ratio, and ventricular remodeling in dogs with myxomatous mitral valve disease.
abstract: OBJECTIVE
  To assess relationships among serum N-terminal procollagen type III concentration, urinary aldosterone-to-creatinine concentration ratio (UAC), and clinical variables in dogs with myxomatous mitral valve disease (MMVD) and healthy dogs.
  
  
  METHODS
  162 dogs with MMVD and 24 healthy control dogs of comparable age and body weight.
  
  
  METHODS
  Blood and urine samples were collected from each dog. Dogs with MMVD underwent echocardiography and ECG. Ventricular diameter measurements were normalized for body weight. Serum N-terminal procollagen type III and urinary aldosterone concentrations were measured via radioimmunoassay. Each dog was examined on 1 to 3 occasions. Examinations were repeated at approximately 6-month intervals.
  
  
  RESULTS
  Serum N-terminal procollagen type III concentration decreased with increasing severity of MMVD and was negatively associated with age and left ventricular end-diastolic and end-systolic diameters. The UAC increased with prior percentage change in left ventricular end-diastolic diameter per month, subsequent percentage change in left ventricular end-systolic diameter per month, and treatment with diuretics and was negatively associated with age. Both UAC and serum N-terminal procollagen type III concentration were higher in Cavalier King Charles Spaniels than in other breeds when other measured variables were controlled for.
  
  
  CONCLUSIONS
  In dogs with MMVD, echocardiographic indicators of left ventricular remodeling appeared to be associated with a decrease in serum concentration of a marker of collagen type III turnover and an increase in urinary aldosterone concentration.
authors.affiliation: Department of Veterinary Clinical Sciences, Royal Veterinary College, University of London, North Mymms, Hatfield, Hertfordshire, AL9 7TA, England. mhezzell@vet.upenn.edu
authors.forename: Melanie J
authors.initials: MJ
authors.lastname: Hezzell
authors.forename: Adrian
authors.initials: A
authors.lastname: Boswood
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Chang
authors.forename: Walasinee
authors.initials: W
authors.lastname: Moonarmart
authors.forename: Jonathan
authors.initials: J
authors.lastname: Elliott
mesh_terms: D000450:Aldosterone / Q000652:urine*; D000818:Animals; D003404:Creatinine / Q000652:urine*; D004283:Dog Diseases / Q000097:blood* / Q000652:urine; D004285:Dogs; D005260:Female; D008297:Male; D008945:Mitral Valve Prolapse / Q000097:blood / Q000652:urine / Q000662:veterinary*; D010446:Peptide Fragments / Q000097:blood*; D011347:Procollagen / Q000097:blood*; D020257:Ventricular Remodeling / Q000502:physiology*
chemical_list: D010446:Peptide Fragments; D011347:Procollagen; C041876:procollagen Type III-N-terminal peptide; D000450:Aldosterone; D003404:Creatinine