title: Effects of two different levels of dietary protein on body composition and protein nutritional status of growing rats.
abstract: This study aimed to investigate the effect of a high-protein diet on growth, body composition, and protein nutritional status of young rats. Newly-weaned Wistar rats, weighing 45-50 g, were distributed in two experimental groups, according to their diets, which contained 12% (G12) or 26% protein (G26), over a period of 3 weeks. The animals were euthanized at the end of this period and the following analyses were performed: chemical composition of the carcass, proteoglycan synthesis, IGF-I concentration (serum, muscle and cartilage), total tissue RNA, protein concentration (muscle and cartilage) and protein synthesis (muscle and cartilage). The high-protein diet was found to result in a higher fat-free mass and lower fat mass in the carcass, with no difference in growth or protein nutritional status.
authors.affiliation: Department of Food Science and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of São Paulo, Brazil. tirapegu@usp.br
authors.forename: Julio
authors.initials: J
authors.lastname: Tirapegui
authors.forename: Sandra Maria Lima
authors.initials: SM
authors.lastname: Ribeiro
authors.forename: Ivanir Santana de Oliveira
authors.initials: IS
authors.lastname: Pires
authors.forename: Marcelo Macedo
authors.initials: MM
authors.lastname: Rogero
mesh_terms: D000818:Animals; D001823:Body Composition; D004044:Dietary Proteins / Q000008:administration & dosage* / Q000097:blood; D007328:Insulin / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000378:metabolism; D018482:Muscle, Skeletal / Q000737:chemistry; D009752:Nutritional Status; D011509:Proteoglycans / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D012709:Serum Albumin / Q000032:analysis
chemical_list: D004044:Dietary Proteins; D007328:Insulin; D011509:Proteoglycans; D012709:Serum Albumin; C548904:insulin-like growth factor-1, rat; D007334:Insulin-Like Growth Factor I
keywords: body composition; dietary protein; growth; protein nutritional status