title: Antiangiogenic activity of indole-3-carbinol in endothelial cells stimulated with activated macrophages.
abstract: The effect of indole-3-carbinol (I3C), a major indolic metabolite in cruciferous vegetables, on lipopolysaccharide (LPS)-activated macrophage-induced tube formation and its associated factors in endothelial EA hy926 cells was investigated. LPS significantly enhanced the capillary-like structure of endothelial cells (ECs) co-cultured with macrophages, but no such effect was observed in single-cultured ECs. I3C, on the other hand, suppressed such enhancement in concert with decreased secretions of vascular endothelial growth factor (VEGF), nitric oxide (NO), interleukin-6 (IL-6), and matrix metalloproteinases (MMPs). The results obtained from cultivating ECs with conditioned medium (CM) collected from macrophages suggested that both ECs and macrophages were inactivated by I3C. These results indicate that I3C from cruciferous vegetables may possess potential roles in preventing inflammation-associated angiogenic diseases.
authors.affiliation: School of Nutrition and Health Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan, ROC.
authors.forename: Mei-Lin
authors.initials: ML
authors.lastname: Wang
authors.forename: Chun-Kuang
authors.initials: CK
authors.lastname: Shih
authors.forename: Hsiao-Pei
authors.initials: HP
authors.lastname: Chang
authors.forename: Yue-Hwa
authors.initials: YH
authors.lastname: Chen
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects*; D051379:Mice
chemical_list: D020533:Angiogenesis Inhibitors; D007211:Indoles; C016517:indole-3-carbinol