title: Non-randomized mtDNA damage after ionizing radiation via charge transport.
abstract: Although it is well known that there are mutation hot spots in mtDNA, whether there are damage hot spots remain elusive. In this study, the regional DNA damage of mitochondrial genome after ionizing radiation was determined by real-time quantitative PCR. The mtDNA damage level was found to be dose-dependent and regional unequal. The control region was the most susceptible region to oxidative damage. GGG, as an typical hole trap during charge transport, was found to be disproportionally enriched in the control region. A total of 107 vertebrate mitochondrial genomes were then analyzed to testify whether the GGG enrichment in control region was evolutionary conserved. Surprisingly, the triple G enrichment can be observed in most of the homeothermal animals, while the majority of heterothermic animals showed no triple G enrichment. These results indicated that the triple G enrichment in control region was related to the mitochondrial metabolism during evolution.
authors.affiliation: Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.forename: Xinguo
authors.initials: X
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Rong
authors.initials: R
authors.lastname: Zhou
authors.forename: Yang
authors.initials: Y
authors.lastname: He
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001482:Base Composition; D002460:Cell Line; D004249:DNA Damage / Q000528:radiation effects*; D004272:DNA, Mitochondrial / Q000145:classification / Q000235:genetics* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D054629:Genome, Mitochondrial; D006801:Humans; D010802:Phylogeny; D011839:Radiation, Ionizing; D014714:Vertebrates / Q000235:genetics
chemical_list: D004272:DNA, Mitochondrial