title: Using major outer membrane protein typing as an epidemiological tool to investigate outbreaks caused by milk-borne Campylobacter jejuni isolates in California.
abstract: We describe using major outer membrane protein (MOMP) typing as a screen to compare the Campylobacter jejuni porA gene sequences of clinical outbreak strains from human stool with the porA sequences of dairy farm strains isolated during two milk-borne campylobacteriosis outbreak investigations in California. The genetic relatedness of clinical and environmental strains with identical or closely related porA sequences was confirmed by multilocus sequence typing and pulsed-field gel electrophoresis analysis. The first outbreak involved 1,644 C. jejuni infections at 11 state correctional facilities and was associated with consumption of pasteurized milk supplied by an on-site dairy (dairy A) at a prison in the central valley. The second outbreak involved eight confirmed and three suspect C. jejuni cases linked to consumption of commercial raw milk and raw chocolate colostrum at another central valley dairy (dairy B). Both dairies bottled fluid milk on the farm and distributed the finished product to off-site locations. Altogether, C. jejuni was isolated from 7 of 15 (46.7%) bovine fecal, 12 of 20 (60%) flush alley water, and 1 of 20 (5%) lagoon samples collected on dairy A. At dairy B, C. jejuni was cultured from 9 of 26 (34.6%) bovine fecal samples. Environmental strains indistinguishable from the clinical outbreak strains were found in five flush alley water samples (dairy A) and four bovine fecal samples (dairy B). The findings demonstrate that MOMP typing is a useful tool to triage environmental isolates prior to conducting more labor-intensive molecular typing methods.
authors.affiliation: University of California, Davis, Davis, California, USA. mjay@ucdavis.edu
authors.forename: Michele T
authors.initials: MT
authors.lastname: Jay-Russell
authors.forename: Robert E
authors.initials: RE
authors.lastname: Mandrell
authors.forename: Jean
authors.initials: J
authors.lastname: Yuan
authors.forename: Anna
authors.initials: A
authors.lastname: Bates
authors.forename: Rosa
authors.initials: R
authors.lastname: Manalac
authors.forename: Janet
authors.initials: J
authors.lastname: Mohle-Boetani
authors.forename: Akiko
authors.initials: A
authors.lastname: Kimura
authors.forename: Janice
authors.initials: J
authors.lastname: Lidgard
authors.forename: William G
authors.initials: WG
authors.lastname: Miller
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques / Q000379:methods*; D002140:California / Q000453:epidemiology; D002169:Campylobacter Infections / Q000453:epidemiology / Q000382:microbiology*; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002417:Cattle; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D004783:Environmental Microbiology; D005517:Foodborne Diseases / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D018272:Porins / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D018272:Porins; C095080:major outer membrane protein, Campylobacter jejuni