title: Expression of PiABP19, Picdc2 and PiSERK3 during induction of somatic embryogenesis in leaflets of Prunus incisa (Thunb.).
abstract: Somatic embryogenesis is a useful tool of plant breeding. In this context, a procedure for inducing somatic embryogenesis in Prunus incisa leaf explants had been previously developed. The original in vitro protocol relies on picloram treatments and exposure to darkness as inductive conditions, the best frequency of embryogenesis being obtained on the second leaf (F(2)) exposed to 4 μM picloram during 30 days. The morphological and biochemical changes observed during somatic embryogenesis occur in response to alterations in gene expression regulation patterns. A molecular study was conducted in order to provide deeper insight into the fundamental biological factors involved in the induction of this process using a gene candidate strategy and semi-quantitative reverse transcription polymerase chain reaction analysis. So far, no sequence data related to somatic embryogenesis has been available in cherry. In the present study, we cloned and sequenced cDNA fragments of putative genes encoding auxin-binding protein, cell cycle regulator and somatic embryogenesis receptor kinase. Time-course differential transcript accumulations were observed for all investigated genes in leaves or derived callus tissues during the observation period (first month of culture). Their possible involvement in the sequential steps of the embryogenic pathway (dedifferentiation, cell proliferation, differentiation through somatic embryogenesis) is presented and discussed.
authors.affiliation: National Agronomic Institute of Tunisia, Cité El Mahrajène, 1082, Tunis, Tunisia. benmahmoud_k21@yahoo.fr
authors.forename: Kaouther
authors.initials: K
authors.lastname: Ben Mahmoud
authors.forename: Fabienne
authors.initials: F
authors.lastname: Delporte
authors.forename: Yordan
authors.initials: Y
authors.lastname: Muhovski
authors.forename: Nadhra
authors.initials: N
authors.lastname: Elloumi
authors.forename: Ahmed
authors.initials: A
authors.lastname: Jemmali
authors.forename: Philippe
authors.initials: P
authors.lastname: Druart
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D048053:Mitogen-Activated Protein Kinase 6 / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000196:embryology / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D058439:Plant Somatic Embryogenesis Techniques; D027861:Prunus / Q000196:embryology / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D010940:Plant Proteins; D048053:Mitogen-Activated Protein Kinase 6