title: Oxygenase-catalyzed ribosome hydroxylation occurs in prokaryotes and humans.
abstract: The finding that oxygenase-catalyzed protein hydroxylation regulates animal transcription raises questions as to whether the translation machinery and prokaryotic proteins are analogously modified. Escherichia coli ycfD is a growth-regulating 2-oxoglutarate oxygenase catalyzing arginyl hydroxylation of the ribosomal protein Rpl16. Human ycfD homologs, Myc-induced nuclear antigen (MINA53) and NO66, are also linked to growth and catalyze histidyl hydroxylation of Rpl27a and Rpl8, respectively. This work reveals new therapeutic possibilities via oxygenase inhibition and by targeting modified over unmodified ribosomes.
authors.affiliation: Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
authors.forename: Wei
authors.initials: W
authors.lastname: Ge
authors.affiliation: Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
authors.forename: Alexander
authors.initials: A
authors.lastname: Wolf
authors.affiliation: Henry Wellcome Building for Molecular Physiology, University of Oxford, Oxford, OX3 7BN, UK.
authors.forename: Tianshu
authors.initials: T
authors.lastname: Feng
authors.affiliation: Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
authors.forename: Chia-Hua
authors.initials: CH
authors.lastname: Ho
authors.affiliation: Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
authors.forename: Rok
authors.initials: R
authors.lastname: Sekirnik
authors.affiliation: Henry Wellcome Building for Molecular Physiology, University of Oxford, Oxford, OX3 7BN, UK.
authors.forename: Adam
authors.initials: A
authors.lastname: Zayer
authors.affiliation: Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Granatino
authors.affiliation: Henry Wellcome Building for Molecular Physiology, University of Oxford, Oxford, OX3 7BN, UK.
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Cockman
authors.affiliation: Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
authors.forename: Christoph
authors.initials: C
authors.lastname: Loenarz
authors.affiliation: Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
authors.forename: Nikita D
authors.initials: ND
authors.lastname: Loik
authors.affiliation: Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
authors.forename: Adam P
authors.initials: AP
authors.lastname: Hardy
authors.affiliation: Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
authors.forename: Timothy D W
authors.initials: TDW
authors.lastname: Claridge
authors.affiliation: Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
authors.forename: Refaat B
authors.initials: RB
authors.lastname: Hamed
authors.affiliation: Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
authors.forename: Rasheduzzaman
authors.initials: R
authors.lastname: Chowdhury
authors.affiliation: Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
authors.forename: Lingzhi
authors.initials: L
authors.lastname: Gong
authors.affiliation: Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
authors.forename: Carol V
authors.initials: CV
authors.lastname: Robinson
authors.affiliation: Biochemistry Dept and Proteomics Core, UT Southwestern Medical Center at Dallas, 6001 Forest Park Rd, Dallas, TX 75390-8816, USA.
authors.forename: David C
authors.initials: DC
authors.lastname: Trudgian
authors.affiliation: Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
authors.forename: Miao
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
authors.forename: Mukram M
authors.initials: MM
authors.lastname: Mackeen
authors.affiliation: Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
authors.forename: James S
authors.initials: JS
authors.lastname: Mccullagh
authors.affiliation: Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
authors.forename: Yuliya
authors.initials: Y
authors.lastname: Gordiyenko
authors.affiliation: Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
authors.forename: Armin
authors.initials: A
authors.lastname: Thalhammer
authors.affiliation: Henry Wellcome Building for Molecular Physiology, University of Oxford, Oxford, OX3 7BN, UK.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamamoto
authors.affiliation: Henry Wellcome Building for Molecular Physiology, University of Oxford, Oxford, OX3 7BN, UK.
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.affiliation: Henry Wellcome Building for Molecular Physiology, University of Oxford, Oxford, OX3 7BN, UK.
authors.forename: Phebee
authors.initials: P
authors.lastname: Liu-Yi
authors.affiliation: Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Clinical Cooperation Unit Molecular Hematology/Oncology, German Cancer Research Center, 69120 Heidelberg, Germany.
authors.forename: Marion
authors.initials: M
authors.lastname: Schmidt-Zachmann
authors.affiliation: Henry Wellcome Building for Molecular Physiology, University of Oxford, Oxford, OX3 7BN, UK.
authors.forename: Benedikt M
authors.initials: BM
authors.lastname: Kessler
authors.affiliation: Henry Wellcome Building for Molecular Physiology, University of Oxford, Oxford, OX3 7BN, UK.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Ratcliffe
authors.affiliation: Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK.
authors.forename: Gail M
authors.initials: GM
authors.lastname: Preston
authors.affiliation: Henry Wellcome Building for Molecular Physiology, University of Oxford, Oxford, OX3 7BN, UK.
authors.forename: Mathew L
authors.initials: ML
authors.lastname: Coleman
authors.affiliation: Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Schofield
mesh_terms: D000818:Animals; D001120:Arginine / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D049308:Dioxygenases; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006639:Histidine / Q000378:metabolism; D056466:Histone Demethylases; D006801:Humans; D006900:Hydroxylation; D009682:Magnetic Resonance Spectroscopy; D006899:Mixed Function Oxygenases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D010105:Oxygenases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011387:Prokaryotic Cells / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D004791:Enzyme Inhibitors; D029968:Escherichia coli Proteins; D009687:Nuclear Proteins; C486160:RPL27A protein, human; D012269:Ribosomal Proteins; C027937:ribosomal protein L16; C056368:ribosomal protein L4; D006639:Histidine; D001120:Arginine; D006899:Mixed Function Oxygenases; D010105:Oxygenases; D049308:Dioxygenases; D056466:Histone Demethylases; C466777:RIOX2 protein, human; C000588756:YcfD protein, E coli; C492859:RIOX1 protein, human