title: Peripheral blood stem cell transplants do not result in endometrial stromal engraftment.
abstract: OBJECTIVE
  To determine whether peripheral blood stem cell transplant (PBSCT) result in engraftment of donor stem cells in the recipient uterus.
  
  
  METHODS
  Prospective clinical and laboratory research.
  
  
  METHODS
  Translational medicine research hospital. PATIENT(S)/ANIMAL(S): Macaque and human bone marrow transplant recipients.
  
  
  METHODS
  Rhesus macaques received autologous transduced immunoselected cytokine-mobilized CD34+ cells after total body irradiation. Vector constructs expressed green fluorescent protein. In the human subjects, prior PBSCT subjects underwent endometrial biopsy and bone marrow aspiration. Macaque and human endometrial and bone marrow cells were isolated and cultured. Fluorescent microscopy, flow cytometry, and polymerase chain reaction (PCR) were used to evaluate for the presence of donor-derived cells.
  
  
  METHODS
  Presence of donor cells in recipient endometrium and bone marrow stroma.
  
  
  RESULTS
  The macaque endometrial cells did not exhibit evidence of green fluorescent protein labeling. Human endometrial cells were cultured and the absence of donor blood contamination was verified. The PCR evaluation of the human endometrial cells did not demonstrate evidence of donor short tandem repeats.
  
  
  CONCLUSIONS
  The PBSCT did not result in engraftment of donor-derived cells in the endometrium.
authors.affiliation: Program in Reproductive and Adult Endocrinology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA. wolffe@mail.nih.gov
authors.forename: Erin F
authors.initials: EF
authors.lastname: Wolff
authors.forename: Naoya
authors.initials: N
authors.lastname: Uchida
authors.forename: Robert E
authors.initials: RE
authors.lastname: Donahue
authors.forename: Mark E
authors.initials: ME
authors.lastname: Metzger
authors.forename: Matthew M
authors.initials: MM
authors.lastname: Hsieh
authors.forename: Lauren L
authors.initials: LL
authors.lastname: Libfraind
authors.forename: Micah J
authors.initials: MJ
authors.lastname: Hill
authors.forename: John F
authors.initials: JF
authors.lastname: Tisdale
mesh_terms: D000818:Animals; D002452:Cell Count; D004717:Endometrium / Q000166:cytology*; D005260:Female; D006801:Humans; D008253:Macaca mulatta; D036102:Peripheral Blood Stem Cell Transplantation