title: Bioassay-guided isolation of urease and α-chymotrypsin inhibitory constituents from the stems of Lawsonia alba Lam. (Henna).
abstract: Seven constituents were isolated from the stems of Lawsonia alba Lam., following an activity-guided isolation, which include two new constituents, namely lawsorosemarinol (1) and lawsofructose (2), one known compound 2-(β-d-glucopyranosyloxy)-1, 4-naphthoquinone (3) and four compounds, 4-hydroxy coumarine (4), 3-(4-hyroxyphenyl)-triacontyl-(Z)-propenoate (5), 3-(4-hydroxy-3-methoxyphenyl)-triacontyl-(Z)-propenoate (6) and 7-hydroxy-4-methyl coumarin (7) first time isolated from Lawsonia alba. Their structure elucidation was based on spectroscopic data analyses. Compounds 3 and 7 showed a moderate inhibition of urease activity, while rest of them showed less than 50% inhibition. These compounds did not show any significant inhibition against α-chymotrypsin.
authors.affiliation: H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan.
authors.forename: Nizam
authors.initials: N
authors.lastname: Uddin
authors.forename: Bina Shaheen
authors.initials: BS
authors.lastname: Siddiqui
authors.forename: Sabira
authors.initials: S
authors.lastname: Begum
authors.forename: Muhammad Imran
authors.initials: MI
authors.lastname: Ali
authors.forename: Bishnu P
authors.initials: BP
authors.lastname: Marasini
authors.forename: Ajmal
authors.initials: A
authors.lastname: Khan
authors.forename: M Iqbal
authors.initials: MI
authors.lastname: Choudhary
mesh_terms: D002918:Chymotrypsin / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D031564:Lawsonia Plant / Q000737:chemistry*; D015394:Molecular Structure; D018547:Plant Stems / Q000737:chemistry*; D013237:Stereoisomerism; D014510:Urease / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D002918:Chymotrypsin; C117608:alpha-chymotrypsin; D014510:Urease