title: Formation and cell translocation of carbon nanotube-fibrinogen protein corona.
abstract: The binding of plasma fibrinogen with both single-walled and multi-walled carbon nanotubes (SWNTs and MWNTs) has been examined. Specifically, our absorbance study indicated that MWNTs were coated with multi-layers of fibrinogen to render a "hard protein corona," while SWNTs were adsorbed with thin layers of the protein to precipitate out of the aqueous phase. In addition, static quenching as a result of energy transfer from fluorescently labeled fibrinogen to their nanotube substrates was revealed by Stern-Volmer analysis. When exposed to HT-29 cells, the nanotubes and fibrinogen could readily dissociate, possibly stemming from their differential affinities for the amphiphilic membrane bilayer.
authors.affiliation: Nano-Biophysics and Soft Matter Laboratory, COMSET, Clemson University, Clemson, South Carolina 29634, USA.
authors.forename: Ran
authors.initials: R
authors.lastname: Chen
authors.forename: Slaven
authors.initials: S
authors.lastname: Radic
authors.forename: Poonam
authors.initials: P
authors.lastname: Choudhary
authors.forename: Kimberley G
authors.initials: KG
authors.lastname: Ledwell
authors.forename: George
authors.initials: G
authors.lastname: Huang
authors.forename: Jared M
authors.initials: JM
authors.lastname: Brown
authors.forename: Pu
authors.initials: P
authors.lastname: Chun Ke