title: Genome update of Botrytis cinerea strains B05.10 and T4.
abstract: We report here an update of the Botrytis cinerea strains B05.10 and T4 genomes, as well as an automated preliminary gene structure annotation. High-coverage de novo assemblies and reference-based alignments led to a correction of wrong base calls, elimination of sequence gaps, and improved joining of contigs. The new assemblies have substantially lower numbers of scaffolds and a concomitant increase in the N(50).The list of protein-coding genes was generated using the evidence-driven gene predictor Augustus, with expressed sequence tag evidence and RNA-Seq data as input.
authors.affiliation: Biosystematics Group, Wageningen University, Wageningen, the Netherlands.
authors.forename: Martijn
authors.initials: M
authors.lastname: Staats
authors.forename: Jan A L
authors.initials: JA
authors.lastname: van Kan
mesh_terms: D001483:Base Sequence; D020171:Botrytis / Q000235:genetics*; D020224:Expressed Sequence Tags; D016681:Genome, Fungal; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA / Q000379:methods*