title: Preliminary studies on the detection ofMycobacterium avium-intracellulare complex using DNA probe from a clinical isolate.
abstract: Genomic DNA from a clinical isolate ofMycobacterium avium-intracellulare complex was purified and cloned in PBR 322 at the tetracycline resistance site using Bam HI restriction enzyme. A 16 kb cloned fragment was purified, radiolabeled and used as a probe. Genomic DNA isolated from nineteen MAC strains, threeM. tuberculosis strains and oneM. kansasii strain were digested with Eco RI restriction enzyme, Southern blotted and hybridized with the 16 kb cloned and labeled fragment. Twelve MAC strains showed positive hybridization although five strains gave faint signals. Positive hybridization was noted in two out of the threeM. tuberculosis strains, possibly due to shared DNA homology. No signal was received from the singleM. kansasii strain used in this study.
authors.affiliation: Department of Biochemistry, Vallabhbhai Patel Chest Institute, University of Delhi, 110007 Delhi.
authors.forename: P
authors.initials: P
authors.lastname: Sharma
authors.forename: M
authors.initials: M
authors.lastname: Bose
authors.forename: I
authors.initials: I
authors.lastname: Mohd
authors.forename: S
authors.initials: S
authors.lastname: Bagdi
authors.forename: H G
authors.initials: HG
authors.lastname: Raj
keywords: DNA probe; Mycobacterium avium-intracellulare (MAC); Southern blotting MAC gene; cloning MAC gene; genomic DNA (MAC)