title: [Absence of poliovirus circulation in Colombian departments with vaccination coverage below 80%].
abstract: This study aims to explore a possible silent circulation of wild and vaccine-derived polioviruses in departments of Colombia with polio vaccination coverage of below 80%. The study collected 52 samples of wastewater concentrated as a result of precipitation with polyethylene glycol and sodium chloride. The viral detection was carried out through isolation and the identification through neutralization of the cytopathic effect, as well as through a conventional polymerase chain reaction following reverse transcription. The isolated polioviruses were characterized by the VP1 gene sequence. In two of the 52 samples, there was a presence of the Sabin type 2 poliovirus with more than 99% sequence similarity with the Sabin type 2 strain polio. Circulation of the nonpolio enterovirus was detected in 17.3% of the samples. The serotypes identified corresponded to coxsackievirus B1, echovirus 30, and echovirus 11. No evidence of the spread of either vaccine-derived poliovirus or wild poliovirus was detected in the departments of Colombia with polio coverage lower than 80%.
authors.affiliation: Universidad del Quindío, Armenia, Colombia. mmgonzalez@uniquindio.edu.co
authors.forename: María Mercedes
authors.initials: MM
authors.lastname: González
authors.forename: Luis
authors.initials: L
authors.lastname: Sarmiento
authors.forename: Gloria Janneth
authors.initials: GJ
authors.lastname: Rey-Benito
authors.forename: Leonardo
authors.initials: L
authors.lastname: Padilla
authors.forename: Alejandra María
authors.initials: AM
authors.lastname: Giraldo
authors.forename: Jhon Carlos
authors.initials: JC
authors.lastname: Castaño
mesh_terms: D000818:Animals; D002460:Cell Line; D003105:Colombia; D004770:Enterovirus / Q000145:classification / Q000302:isolation & purification; D006801:Humans; D051379:Mice; D017955:Poliovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D011055:Poliovirus Vaccine, Oral; D012703:Serotyping; D014611:Vaccination / Q000706:statistics & numerical data*; D014776:Virus Cultivation; D062065:Wastewater / Q000821:virology*
chemical_list: D011055:Poliovirus Vaccine, Oral; D062065:Wastewater