title: Correlation between salivary alpha-amylase and stress-related anxiety.
abstract: Salivary alpha-amylase is a useful biomarker that can be used in assessing human psychobiological and social behavioural processes. Studying it opens up possibilities for the creation of novel concepts concerning the interaction of biological and social processes and their impact on health and behaviour.
  
  
  METHODS
  The levels of salivary alpha-amylase and situation anxiety self-assessment using Spielberger test were measured twice in 30 individuals aged 21.37 +/- 0.96 yrs (18 females and 12 males): once during stressful situation (prior to examination) and, again a month later, in stress-free environment (during a training session). Salivary alpha-amylase was measured using kinetic reaction kit Salimetrics LLC--USA.
  
  
  RESULTS
  The mean level of salivary alpha-amylase measured during the first measurement 156.0 +/- 93.33 U/ml. During the second measurement in the absence of intense stress, the levels were two times lower - 74.03 +/- 58.06 U/ml and the differences were statistically significant (P < 0.001). We found a statistically significant correlation between the levels of salivary alpha-amylase in both measurements (P < 0.01). The correlation coefficient was r = 0.472 (P < 0.01).
  
  
  CONCLUSIONS
  The adapted version of the State-Trait Anxiety Inventory score (STAI) created by Spielberger is appropriate for assessment of stress-related anxiety in young individuals. Salivary alpha-amylase may be used as a biomarker for objective evaluation of the psychosomatic state of individuals in a stressful environment. The combination of psychological test and objective indicator such as salivary alpha-amylase is an excellent tool for objective evaluation of individual's state in stressful environment. Similar tests may be used in assessment of patients' behaviours at dental treatment that may be considered a stressor in most patients.
authors.affiliation: Department of Pediatric Dentistry, Faculty of Dental Medicine, Medical University, Sofia, Bulgaria. mayarashkova@mail.bg
authors.forename: Maya R
authors.initials: MR
authors.lastname: Rashkova
authors.forename: Lora S
authors.initials: LS
authors.lastname: Ribagin
authors.forename: Nina G
authors.initials: NG
authors.lastname: Toneva
mesh_terms: D000328:Adult; D000681:Amylases / Q000032:analysis*; D001007:Anxiety / Q000201:enzymology*; D015415:Biomarkers / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D011569:Psychiatric Status Rating Scales; D012463:Saliva / Q000201:enzymology*; D013315:Stress, Psychological / Q000201:enzymology*
chemical_list: D015415:Biomarkers; D000681:Amylases