title: Mapping histological levels of 8-hydroxy-2'-deoxyguanosine in female reproductive organs.
abstract: Oxidative stress is associated with many disease states including gynecologic disease. This process can damage lipids, proteins and DNA. The present study highlights the role of oxidative stress induced DNA damage as measured by 8-hydroxy-2-deoxyguanosine in development of benign gynecological conditions (BGC). Our aim was to map the oxidative DNA damage on female reproductive organs and highlight the high amount found in a variety of benign gynecologic disorders. Seventeen biopsy specimens from female pelvic organs were divided in two groups: healthy organs tissue and BGC tissue. Healthy organs biopsy tissue included the cervix, tubes, uterus, peritoneum, and topic endometrium in secretory phase. Benign gynecological biopsy tissue included hydrosalpinges, leiomyoma, adenomyosis and tubal cysts. Immunohistochemical staining showed significantly higher levels of DNA damage between BGC and healthy organs [19.36 % (6.20; 32.51) vs. 4.61 % (0.63; 8.53); P < 0.0344]. Our results highlight the involvement of oxidative stress DNA damage in female benign pelvic disease. Hydrosalpinges, leiomyoma, and adenomyosis exhibit the highest amounts of oxidative DNA damage in the pelvic cavity.
authors.affiliation: Center for Reproductive Medicine, Obstetrics and Gynecology and Women's Health Institute, Glickman Urology and Kidney Institute, Cleveland, OH 44195, USA. luizcarvalho.dr@me.com
authors.forename: Luiz Fernando Pina
authors.initials: LF
authors.lastname: de Carvalho
authors.forename: Mauricio Simões
authors.initials: MS
authors.lastname: Abrão
authors.forename: Charles
authors.initials: C
authors.lastname: Biscotti
authors.forename: Rakesh
authors.initials: R
authors.lastname: Sharma
authors.forename: Ashok
authors.initials: A
authors.lastname: Agarwal
authors.forename: Tommaso
authors.initials: T
authors.lastname: Falcone
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D062788:Adenomyosis / Q000378:metabolism / Q000473:pathology; D000328:Adult; D002584:Cervix Uteri / Q000166:cytology / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D005260:Female; D005836:Genitalia, Female / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine