title: Construction of a doxycycline inducible adipogenic lentiviral expression system.
abstract: To provide a tool for research on regulating adipocyte differentiation, tetracycline inducible (Tet on) lentiviral expression vectors under the control of an adipose-specific promoter were constructed. The lowest basal expression in the absence of doxycycline and most efficient dose-dependent, doxycycline-induced transient overexpression was observed using vectors constructed with a combination of Tetracycline Responsive Element (TRE) and reverse tetracycline-controlled TransActivator advanced (rtTAadv), transfected in white (3T3-L1) and brown (HIB-1B) preadipocytes cell lines. The results demonstrate that doxycycline adipogenic inducible expression can be achieved using a pLenti TRE / rtTA adv under the control of the truncated aP2 promoter in HIB-1B preadipocytes.
authors.affiliation: School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, United Kingdom.
authors.forename: Q
authors.initials: Q
authors.lastname: Liu
authors.forename: P J
authors.initials: PJ
authors.lastname: Hill
authors.forename: A
authors.initials: A
authors.lastname: Karamitri
authors.forename: K J P
authors.initials: KJ
authors.lastname: Ryan
authors.forename: H Y
authors.initials: HY
authors.lastname: Chen
authors.forename: M A
authors.initials: MA
authors.lastname: Lomax
mesh_terms: D041721:3T3-L1 Cells; D052437:Adipocytes, Brown / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D050156:Adipogenesis; D000818:Animals; D004318:Doxycycline / Q000378:metabolism / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D005822:Genetic Vectors / Q000235:genetics*; D016086:Lentivirus / Q000235:genetics*; D051379:Mice; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D013752:Tetracycline; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D015534:Trans-Activators; D013752:Tetracycline; D004318:Doxycycline