title: Interaction analysis identifies semenogelin I fragments as new binding partners of PIP in human seminal plasma.
abstract: Identification of protein-protein interactions is vital for complete understanding of a biological process and for functional characterization of a protein in related biochemical pathways. In this study, we performed analysis of prolactin inducible protein (PIP) interactions in human seminal plasma. PIP and its interacting partners were co-immunoprecipitated, analyzed by SDS-PAGE and identified by MALDI-TOF mass spectrometry. Three major interacting partners were identified, viz. human serum albumin, zinc-α-2 glycoprotein and semenogelin I fragments. This is the first report of interaction between PIP and semenogelin I fragments in human seminal plasma or elsewhere with a suggestive role in reproductive physiology which might be helpful for spermatozoa to acquire their motility.
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Anil Kumar
authors.initials: AK
authors.lastname: Tomar
authors.forename: Balwinder Singh
authors.initials: BS
authors.lastname: Sooch
authors.forename: Isha
authors.initials: I
authors.lastname: Raj
authors.forename: Sarman
authors.initials: S
authors.lastname: Singh
authors.forename: Savita
authors.initials: S
authors.lastname: Yadav
mesh_terms: D000328:Adult; D006801:Humans; D008297:Male; D012661:Semen / Q000737:chemistry / Q000378:metabolism*; D029589:Seminal Plasma Proteins / Q000737:chemistry / Q000378:metabolism*; D029607:Seminal Vesicle Secretory Proteins / Q000737:chemistry / Q000378:metabolism*; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism
chemical_list: D029589:Seminal Plasma Proteins; D029607:Seminal Vesicle Secretory Proteins; C424482:seminal vesicle-specific antigen