title: Enhanced-acceptor fluorescence-based single cell ATP biosensor monitors ATP in heterogeneous cancer populations in real time.
abstract: Current methods to monitor cellular ATP do not provide spatial or temporal localization of ATP in single cells in real time or they display imperfect specificity to ATP. Here, we have developed a single cell, Enhanced Acceptor Fluorescence (EAF)-based ATP biosensor to visualize ATP in real time. This biosensor utilizes a modified mimic of the ε-subunits of the Bacillus subtilis F(0)F(1) synthase and is coupled to the EAF fluorophores pairs, GFP and YFP. The sensor was then used to monitor ATP in a heterogeneous glioblastoma multiform cancer cell population. We anticipate that this innovative technology and our better understanding of the ATP machinery will have substantial influence on future translational studies.
authors.affiliation: David Geffen School of Medicine, University of California-Los Angeles, 10833 Le Conte Ave., Los Angeles, CA 90089, USA. szadran@mednet.ucla.edu
authors.forename: Sohila
authors.initials: S
authors.lastname: Zadran
authors.forename: Desiree
authors.initials: D
authors.lastname: Sanchez
authors.forename: Homera
authors.initials: H
authors.lastname: Zadran
authors.forename: Arash
authors.initials: A
authors.lastname: Amighi
authors.forename: Erick
authors.initials: E
authors.lastname: Otiniano
authors.forename: Kaylee
authors.initials: K
authors.lastname: Wong
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D001412:Bacillus subtilis / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D003584:Cytological Techniques / Q000379:methods*; D005453:Fluorescence; D005909:Glioblastoma / Q000503:physiopathology*; D006801:Humans; D006180:Proton-Translocating ATPases / Q000378:metabolism
chemical_list: D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases