title: Scattering anisotropy-weighted mesoscopic imaging.
abstract: We report that when tissue images are formed via a small solid angle in the backward direction (i.e., back-directional gating), the image intensity is dominantly determined by tissue scattering anisotropy. Thus, this configuration allows for scattering anisotropy-weighted imaging that can provide an intrinsic contrast by capturing tissue structures and organizations. To demonstrate the immediate feasibility, we apply scattering anisotropy-weighted imaging to tissue blocks including basal-cell carcinomas as a pilot study. The main feature of our imaging approach is the high sensitivity to tumor locations and the simplicity for large-area visualization. We further envision that scattering anisotropy-weighted imaging could potentially be used to visualize tissue microenvironments in a mesoscopic (between microscopic and macroscopic) imaging setting.
authors.forename: Zhengbin
authors.initials: Z
authors.lastname: Xu
authors.forename: Ally-Khan
authors.initials: AK
authors.lastname: Somani
authors.forename: Young L
authors.initials: YL
authors.lastname: Kim
mesh_terms: D000465:Algorithms; D003198:Computer Simulation; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008027:Light; D008853:Microscopy / Q000379:methods*; D008954:Models, Biological; D015233:Models, Statistical; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity