title: Stochastic differentiation into an osteoclast lineage from cloned macrophage-like cells.
abstract: Differentiation into osteoclasts is induced by a macrophage colony-stimulating factor and receptor activator of nuclear-factor κB ligand. The macrophage-like cell line, C7 has the potential to differentiate into osteoclasts when it is cultured with both factors for 6 days. Although C7 is an established cell line, the frequency of differentiation into this lineage was less than 10%, and the ratio was maintained at a constant level, even after repeated cloning. In this study, to increase the differentiation of C7 cells to osteoclasts, C7 derivative treatments with several activators and/or inhibitors were performed for 3 days prior to setting osteoclast induction analysis; however, a reagent to significantly up-regulate the frequency of differentiation was not found. Only extended cultures for osteoclastogenesis exponentially increased the frequency of osteoclast precursors. It is likely that C7 cell differentiation into committed osteoclast precursors is on 'autopilot' rather than requiring specific signals to drive this process.
authors.affiliation: Division of Immunology, Department of Molecular and Cellular Biology, School of Life Science, Faculty of Medicine, Tottori University, 86 Nishi-Cho, Yonago, Tottori 683-8503, Japan. shayashi@med.tottori-u.ac.jp
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Hayashi
authors.forename: Akihiko
authors.initials: A
authors.lastname: Murata
authors.forename: Kazuki
authors.initials: K
authors.lastname: Okuyama
authors.forename: Yuhki
authors.initials: Y
authors.lastname: Shimoda
authors.forename: Mari
authors.initials: M
authors.lastname: Hikosaka
authors.forename: Hisataka
authors.initials: H
authors.lastname: Yasuda
authors.forename: Miya
authors.initials: M
authors.lastname: Yoshino
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D019070:Cell Lineage; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D010010:Osteoclasts / Q000166:cytology*; D013269:Stochastic Processes
chemical_list: D016173:Macrophage Colony-Stimulating Factor