title: Signaling regulated endocytosis and exocytosis lead to mating pheromone concentration dependent morphologies in yeast.
abstract: Polarized cell morphogenesis requires actin cytoskeleton rearrangement for polarized transport of proteins, organelles and secretory vesicles, which fundamentally underlies cell differentiation and behavior. During yeast mating, Saccharomyces cerevisiae responds to extracellular pheromone gradients by extending polarized projections, which are likely maintained through vesicle transport to (exocytosis) and from (endocytosis) the membrane. We experimentally demonstrate that the projection morphology is pheromone concentration-dependent, and propose the underlying mechanism through mathematical modeling. The inclusion of membrane flux and dynamically evolving cell boundary into our yeast mating signaling model shows good agreement with experimental measurements, and provides a plausible explanation for pheromone-induced cell morphology.
authors.affiliation: Department of Mathematics, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Ching-Shan
authors.initials: CS
authors.lastname: Chou
authors.affiliation: Center for Complex Biological Systems, University of California at Irvine, Irvine, CA 92697, USA.|Department of Developmental and Cell Biology, University of California at Irvine, Irvine, CA 92697, USA.
authors.forename: Travis I
authors.initials: TI
authors.lastname: Moore
authors.affiliation: Department of Developmental and Cell Biology, University of California at Irvine, Irvine, CA 92697, USA.
authors.forename: Steven D
authors.initials: SD
authors.lastname: Chang
authors.affiliation: Center for Complex Biological Systems, University of California at Irvine, Irvine, CA 92697, USA.|Department of Mathematics, University of California at Irvine, Irvine, CA 92697, USA.
authors.forename: Qing
authors.initials: Q
authors.lastname: Nie
authors.affiliation: Center for Complex Biological Systems, University of California at Irvine, Irvine, CA 92697, USA.|Department of Developmental and Cell Biology, University of California at Irvine, Irvine, CA 92697, USA.|Department of Molecular, Cellular, and Developmental Biology, University of California at Santa Barbara, Santa Barbara, CA 93106, USA.
authors.forename: Tau-Mu
authors.initials: TM
authors.lastname: Yi
mesh_terms: D004705:Endocytosis / Q000502:physiology*; D005089:Exocytosis / Q000502:physiology*; D008962:Models, Theoretical; D010675:Pheromones / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism* / Q000502:physiology*
chemical_list: D010675:Pheromones