title: [Catalytic properties of a nitrile hydratase immobilized on activated chitosan].
abstract: The catalytic properties of a nitrile hydratase, isolated from a strain of Rhodococcus ruber gt1 and immobilized by covalent cross-linking with chitosan activated with 0.1% benzoquinone solution, have been investigated. The kinetic parameters ofacrylonitrile hydration catalyzed by immobilized nitrile hydratase and the enzyme in a solution have been determined. It is found that the immobilization does not lead to a decrease in the maximum reaction rate (Vmax), whereas the Michaelis constant (K(M)) is reduced by a factor of 2.4. The possibility of reusing an immobilized enzyme for 50 consecutive cycles of acrylonitrile transformation was shown, and the nitrile hydratase activity in the 50th cycle exceeded that in the first cycle by 3.5 times. It is shown that the effect of temperature on activity depended on the concentration of the enzyme, which confirms the dissociative nature of nitrile hydratase inactivation. It was found that immobilized nitrile hydratases remain active at pH 3.0-4.0, whereas the enzyme is inactivated in a solution under these conditions. The resulting biocatalyst can be effectively used to receive acrylamide from acrylonitrile.
authors.forename: Iu G
authors.initials: IuG
authors.lastname: Maksimova
authors.forename: T A
authors.initials: TA
authors.lastname: Rogozhnikova
authors.forename: G V
authors.initials: GV
authors.lastname: Ovechkina
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Maksimov
authors.forename: V A
authors.initials: VA
authors.lastname: Demakov
mesh_terms: D020106:Acrylamide / Q000737:chemistry / Q000378:metabolism; D000181:Acrylonitrile / Q000737:chemistry / Q000378:metabolism; D002384:Catalysis; D048271:Chitosan / Q000378:metabolism*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006836:Hydro-Lyases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012240:Rhodococcus / Q000201:enzymology; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D020106:Acrylamide; D048271:Chitosan; D006836:Hydro-Lyases; C048831:nitrile hydratase; D000181:Acrylonitrile