title: Development and validation of a 'universal' HPLC method for pyrethroid quantification in long-lasting insecticidal mosquito nets for malaria control and prevention.
abstract: OBJECTIVE
  To outline the development and validation of a universal method for quantifying deltamethrin, permethrin and alpha-cypermethrin levels in a variety of long-lasting insecticidal mosquito nets.
  
  
  METHODS
  Using the HPLC conditions found in the CIPAC method for deltamethrin quantification, the method is based on a simple extraction technique for sample preparation (heating in isooctane at approximately 100 °C for 15 min). The method was validated for linearity, specificity, accuracy, precision, insecticide stability to extraction conditions and required extraction time for insecticide removal.
  
  
  RESULTS
  The method was found valid for insecticide quantifications for various types of nets, namely for deltamethrin coated on polyester nets, deltamethrin incorporated into polyethylene nets, permethrin incorporated into polyethylene nets, alpha-cypermethrin coated on polyester nets and alpha-cypermethrin incorporated into polyethylene nets.
  
  
  CONCLUSIONS
  This method will provide a more simplified approach to testing a variety of nets (different types of fibre) containing deltamethrin, permethrin or alpha-cypermethrin.
authors.affiliation: Product Quality and Compliance, FHI 360, Durham, NC, USA.
authors.forename: David W
authors.initials: DW
authors.lastname: Jenkins
authors.forename: Arno
authors.initials: A
authors.lastname: Hensens
authors.forename: Jacob
authors.initials: J
authors.lastname: Lloyd
authors.forename: Michael
authors.initials: M
authors.lastname: Payne
authors.forename: Peter
authors.initials: P
authors.lastname: Cizmarik
authors.forename: Steve
authors.initials: S
authors.lastname: Hamel
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009033:Culicidae; D006801:Humans; D017053:Infection Control; D007306:Insecticides / Q000032:analysis*; D008288:Malaria / Q000517:prevention & control*; D009032:Mosquito Control / Q000379:methods*; D057147:Mosquito Nets; D009570:Nitriles / Q000032:analysis; D026023:Permethrin / Q000032:analysis; D011091:Polyesters; D020959:Polyethylene; D011722:Pyrethrins / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D007306:Insecticides; D009570:Nitriles; D011091:Polyesters; D011722:Pyrethrins; C017160:cypermethrin; C017180:decamethrin; D026023:Permethrin; D020959:Polyethylene