title: Supramolecular immobilization of glucose oxidase on gold coated with cyclodextrin-modified cysteamine core PAMAM G-4 dendron/Pt nanoparticles for mediatorless biosensor design.
abstract: Cysteamine core polyamidoamine G-4 dendron branched with β-cyclodextrins was chemisorbed on the surface of Au electrodes and further coated with Pt nanoparticles. Adamantane-modified glucose oxidase was subsequently immobilized on the nanostructured electrode surface by supramolecular association. This enzyme electrode was used to construct a reagentless amperometric biosensor for glucose, making use of the electrochemical oxidation of H2O2 generated in the enzyme reaction. The amperometric response of the biosensor was rapid (6 s) and a linear function of glucose concentration between 5 and 705 μmol L(-1). The biosensor had a low detection limit of 2.0 μmol L(-1), sensitivity of 197 mA mol(-1) L cm(-2), and retained 94% of its initial response after storage for nine days at 4 °C.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040 Madrid, Spain.
authors.forename: Paula
authors.initials: P
authors.lastname: Díez
authors.forename: Ciprian-George
authors.initials: CG
authors.lastname: Piuleac
authors.forename: Paloma
authors.initials: P
authors.lastname: Martínez-Ruiz
authors.forename: Santiago
authors.initials: S
authors.lastname: Romano
authors.forename: María
authors.initials: M
authors.lastname: Gamella
authors.forename: Reynaldo
authors.initials: R
authors.lastname: Villalonga
authors.forename: José M
authors.initials: JM
authors.lastname: Pingarrón
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003543:Cysteamine / Q000737:chemistry*; D050091:Dendrimers / Q000737:chemistry*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry; D010984:Platinum / Q000737:chemistry; D012680:Sensitivity and Specificity; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D050091:Dendrimers; D004800:Enzymes, Immobilized; C104700:PAMAM Starburst; D047392:beta-Cyclodextrins; D010984:Platinum; D003543:Cysteamine; D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose