title: Interaction between natural compounds and human topoisomerase I.
abstract: Eukaryotic topoisomerase I (Top1) is a monomeric enzyme that catalyzes the relaxation of supercoiled DNA during important processes including DNA replication, transcription, recombination and chromosome condensation. Human Top1 I is of significant medical interest since it is the unique cellular target of camptothecin (CPT), a plant alkaloid that rapidly blocks both DNA and RNA synthesis. In this review, together with CPT, we point out the interaction between human Top1 and some natural compounds, such us terpenoids, flavonoids, stilbenes and fatty acids. The drugs can interact with the enzyme at different levels perturbing the binding, cleavage, rotation or religation processes. Here we focus on different assays that can be used to identify the catalytic step of the enzyme inhibited by different natural compounds.
authors.affiliation: Department of Biology, University of Rome Tor Vergata, Via Della Ricerca Scientifica, I-00133 Rome, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Castelli
authors.forename: Andrea
authors.initials: A
authors.lastname: Coletta
authors.forename: Ilda
authors.initials: I
authors.lastname: D'Annessa
authors.forename: Paola
authors.initials: P
authors.lastname: Fiorani
authors.forename: Cinzia
authors.initials: C
authors.lastname: Tesauro
authors.forename: Alessandro
authors.initials: A
authors.lastname: Desideri
mesh_terms: D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology*; D004264:DNA Topoisomerases, Type I / Q000737:chemistry / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry / Q000494:pharmacology*; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008958:Models, Molecular; D015394:Molecular Structure; D013267:Stilbenes / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013729:Terpenes / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D001688:Biological Products; D005227:Fatty Acids; D005419:Flavonoids; D013267:Stilbenes; D013729:Terpenes; D004264:DNA Topoisomerases, Type I