title: Thermodynamically unfavorable DNA hybridizations can be made to occur by a water to ice phase change.
abstract: In an apparent contradiction to Debye-Hückel theory, it was possible to hybridize DNA in solutions of Milli-Q water (resistivity>18MΩcm(-1)) containing no added ions. This was demonstrated by hybridizing four bi-complementary DNA sequences to form an 'X' shape, as indicated by acrylamide gel electrophoresis. The requirement for hybridization was that a water-to-ice phase change should occur. Comparative experiments, using freezing by liquid nitrogen and thawing at different temperatures, showed that hybridization could take place during either the freezing or thawing process provided either was slow enough. We speculate that the low solubility of DNA in ice creates liquid inclusions of extremely high DNA and counter-ion concentration prior to complete freezing, and that hence in these inclusions hybridization was actually in accordance with Debye-Hückel theory.
authors.affiliation: School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkhuntien Campus, Bangkok 10150, Thailand.
authors.forename: Abhichart
authors.initials: A
authors.lastname: Krissanaprasit
authors.forename: Cristian
authors.initials: C
authors.lastname: Guajardo
authors.forename: Mithran
authors.initials: M
authors.lastname: Somasundrum
authors.forename: Werasak
authors.initials: W
authors.lastname: Surareungchai
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D005615:Freezing; D007053:Ice / Q000032:analysis; D007477:Ions / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012995:Solubility; D013816:Thermodynamics; D014867:Water / Q000737:chemistry*
chemical_list: D007053:Ice; D007477:Ions; D014867:Water; D004247:DNA