title: Differential immune response in mice immunized with the A, R or C domain from TcSP protein of Trypanosoma cruzi or with the coding DNAs.
abstract: In a murine model of experimental Trypanosoma cruzi (H8 strain) infection, we investigated the induction of protective immunity against the domains [amino (A), repeats (R) and carboxyl (C)] of the surface protein (SP), a member of the trans-sialidase (TS) superfamily. Recombinant proteins and plasmid DNA coding for the respective proteins were used to immunize BALB/c mice, and the humoral response and cytokine levels were analysed. Immunization with the recombinant proteins induced higher levels of anti-TcSP antibodies than immunization with the corresponding DNAs, and analysis of serum cytokines showed that immunization with both recombinant proteins and naked DNA resulted in a Th1-Th2 mixed T-cell response. Mice immunized with either recombinant proteins or plasmid DNA were infected with blood trypomastigotes. The recombinant protein-immunized mice showed a variable reduction in peak parasitemia, and most died by day 60. Only the pBKTcSPR-immunized mice exhibited a significant reduction in peak parasitemia and survived the lethal challenge. DNA-based immunization with DNA coding for the repeats domain of TcSP is a good candidate for the development of a vaccine against experimental T. cruzi infection.
authors.affiliation: Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del IPN, México, D.F, México.
authors.forename: B
authors.initials: B
authors.lastname: Salgado-Jiménez
authors.forename: M
authors.initials: M
authors.lastname: Arce-Fonseca
authors.forename: L
authors.initials: L
authors.lastname: Baylón-Pacheco
authors.forename: P
authors.initials: P
authors.lastname: Talamás-Rohana
authors.forename: J L
authors.initials: JL
authors.lastname: Rosales-Encina
mesh_terms: D000818:Animals; D016207:Cytokines / Q000276:immunology; D004247:DNA / Q000235:genetics / Q000276:immunology; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D016052:Protozoan Vaccines / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014349:Trypanosoma cruzi / Q000276:immunology*; D014352:Trypanosomiasis / Q000276:immunology*
chemical_list: D016207:Cytokines; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D011994:Recombinant Proteins; C501457:SSP4 protein, Trypanosoma cruzi; D004247:DNA