title: Detection of trace processes in the networks of neurons cultured on microelectrode arrays.
abstract: We compared the effects of electrical stimulation of primary dissociated neuronal cultures cultured on microelectrode arrays in terms on the expression of c-fos transcriptional factor that is involved in plastic reorganization in neurons. Dissociated hippocampal neurons cultured on multielectrode arrays were exposed to two stimulation protocols: high-frequency and low-frequency stimulations. Expression of c-fos was evaluated using immunofluorescence. Both high-frequency and low-frequency stimulations significantly increased c-fos expression in comparison with non-stimulated control. These findings indicate that c-fos expression can be induced in neuronal cell culture by different types of electrical stimulations and can be used for studying plasticity processes in microphysiological in vitro systems.
authors.affiliation: Department of Neuroscience, NBICS Center, National Research Center Kurchatov Institute, Laboratory of Neurobiology of Memory, PK Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences, Moscow, Russia. alsukhanova@gmail.com
authors.forename: A L
authors.initials: AL
authors.lastname: Sukhanova
authors.forename: O A
authors.initials: OA
authors.lastname: Mineyeva
authors.forename: I I
authors.initials: II
authors.lastname: Kiselev
authors.forename: M S
authors.initials: MS
authors.lastname: Burtsev
authors.forename: K V
authors.initials: KV
authors.lastname: Anokhin
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D004558:Electric Stimulation; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000502:physiology*; D006624:Hippocampus / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008839:Microelectrodes; D009473:Neuronal Plasticity / Q000502:physiology*; D009474:Neurons / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*
chemical_list: D016760:Proto-Oncogene Proteins c-fos