title: Detection of tubercular antibody and antigen in sera of bone and joint tuberculosis.
abstract: Trichloroacetic acid (TCA) solubilized and DEAE fractionatedMycobacterium tuberculosis H(37)Ra excretory-secretory (ES) antigen viz., Mtb EST DE1 and affinity purified goat antibodies to the TCA solubilized ES antigen (Mtb EST) were explored in detecting tubercular antibody and antigen respectively in sera of bone and joint tuberculosis by indirect and sandwich ELISA. Out of total 36 bone & joint tuberculosis cases, tubercular antibody was detected by indirect ELISA in 30 patients (sensitivity 83%), while circulating tubercular antigen was detected by sandwich ELISA in 27 patients (sensitivity 75%). Out of 34 non tubercular disease control cases, 10 patients showed positive reaction for antibody while only 4 patients showed positive reaction for antigen. In another group of 34 healthy subjects who were screened, 4 individuals showed positive reaction for tubercular antibody and 2 cases for antigen. This study shows that antigen detection assay using affinity purified anti Mtb EST antigen antibody is superior with overall specificity of 91% as compared to antibody detection assay with 75% specificity in bone & joint tuberculosis.
authors.affiliation: Jamnalal Bajaj Tropical Disease Research Centre & Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, 442 102 Sevagram (Wardha).
authors.forename: J
authors.initials: J
authors.lastname: Pramanik
authors.forename: A N
authors.initials: AN
authors.lastname: Lodam
authors.forename: C M
authors.initials: CM
authors.lastname: Badole
authors.forename: M V
authors.initials: MV
authors.lastname: Reddy
authors.forename: K R
authors.initials: KR
authors.lastname: Patond
authors.forename: B C
authors.initials: BC
authors.lastname: Harinath
keywords: Mycobacterium tuberculosis; anti mtb EST antigen; antibody; bone & joint tuberculosis; excretroy-secretory antigen; indirect ELISA; sandwich ELISA