title: LC-MS-based method for the qualitative and quantitative analysis of the novel PPARγ agonist KR-62980.
abstract: A sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed for a novel peroxisome proliferator-activated receptor γ (PPARγ) agonist, KR-62980, in rat plasma. It involves liquid-liquid extraction (LLE) followed by HPLC separation and electrospray ionization tandem mass spectrometry. The linear ranges of the assay were 0.01-10 μg/mL with a correlation coefficient (R               (2)) greater than 0.99 and the lower limit of quantification was 0.01 μg/mL. The average recovery was 90.1 and 98.4% from rat plasma for KR-62980 and imipramine, respectively. The coefficients of variation of intra- and inter-assay were 1.2-10.6% and the relative error was 0.8-13.2%. The method was validated and successfully applied to the pharmacokinetic study of KR-62980 in rat.
authors.affiliation: Drug Discovery Platform Technology Team, Medicinal Science Division, Korea Research Institute of Chemical Technology, Daejon, South Korea.
authors.forename: Min-Sun
authors.initials: MS
authors.lastname: Kim
authors.forename: Myung Ae
authors.initials: MA
authors.lastname: Bae
mesh_terms: D053000:Analytic Sample Preparation Methods; D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D007192:Indenes / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000494:pharmacology*; D059625:Liquid-Liquid Extraction; D008297:Male; D009025:Morpholines / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000494:pharmacology*; D047495:PPAR gamma / Q000819:agonists*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C513115:1-(methylimino-N-oxy)-6-(2-morpholinoethoxy)-3-phenyl-1H-indene-2-carboxylic acid ethyl ester; D007192:Indenes; D009025:Morpholines; D047495:PPAR gamma