title: IRIS iQ200 workstation as a screen for performing urine culture.
abstract: We cultured 1000 urines and tested them with the iQ200 Workstation (Iris Diagnostics, Chatsworth, CA, and Arkray, Edina, MN, USA) for leukocyte esterase and nitrite (LE/N), white blood cells (WBC), all small particles (ASP), bacteria, and yeast. Positive culture grew ≥ 10(4) CFU/mL of 1 or 2 uropathogens. Method A assessed LE/N, WBCs ≥ 6/μL, and bacteria/yeast. Method B assessed WBCs ≥ 6/μL, bacteria/yeast, and ASP ≥ 10,000. Each was compared to culture results, clinician's diagnosis (CD), or expert review (ER). Sensitivities by culture were 88.7% (Method A) and 88.3% (Method B), with negative predictive values (NPVs) of 94.4% and 94.2%, respectively. Sensitivity and NPV increased for comparison to the CD (99.6%) and NPVs of 99.0% and 99.3% compared to ER. ASP did not enhance specificity, sensitivity, or NPV. The iQ200 Workstation performed well by any standard, thus providing a reliable system by which to improve the use of laboratory resources.
authors.affiliation: Medical Care Line (Infectious Disease Section), Michael E. DeBakey Veterans Affairs Medical Center, Houston, TX 77030, USA. parta@bcm.edu
authors.forename: Mark
authors.initials: M
authors.lastname: Parta
authors.forename: Bernita Y
authors.initials: BY
authors.lastname: Hudson
authors.forename: Thao P
authors.initials: TP
authors.lastname: Le
authors.forename: Michael
authors.initials: M
authors.lastname: Ittmann
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Musher
authors.forename: Charles
authors.initials: C
authors.lastname: Stager
mesh_terms: D001419:Bacteria / Q000302:isolation & purification; D058491:Bacterial Load; D002265:Carboxylic Ester Hydrolases / Q000032:analysis; D015169:Colony Count, Microbial; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008828:Microbiological Techniques / Q000379:methods*; D009573:Nitrites / Q000032:analysis; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D014552:Urinary Tract Infections / Q000175:diagnosis* / Q000382:microbiology*; D014556:Urine / Q000382:microbiology*; D015003:Yeasts / Q000302:isolation & purification
chemical_list: D009573:Nitrites; C021443:leukocyte esterase; D002265:Carboxylic Ester Hydrolases