title: Comparative proteomic analysis of Lactobacillus plantarum WCFS1 and ΔctsR mutant strains under physiological and heat stress conditions.
abstract: Among Gram-positive bacteria, CtsR (Class Three Stress gene Repressor) mainly regulates the expression of genes encoding the Clp ATPases and the ClpP protease. To gain a better understanding of the biological significance of the CtsR regulon in response to heat-shock conditions, we performed a global proteomic analysis of Lactobacillus plantarum WCFS1 and ΔctsR mutant strains under optimal or heat stress temperatures. Total protein extracts from bacterial cells were analyzed by two-dimensional gel fractionation. By comparing maps from different culture conditions and different L. plantarum strains, image analysis revealed 23 spots with altered levels of expression. The proteomic analysis of L. plantarum WCFS1 and ctsR mutant strains confirms at the translational level the CtsR-mediated regulation of some members of the Clp family, as well as the heat induction of typical stress response genes. Heat activation of the putative CtsR regulon genes at transcriptional and translational levels, in the ΔctsR mutant, suggests additional regulative mechanisms, as is the case of hsp1. Furthermore, isoforms of ClpE with different molecular mass were found, which might contribute to CtsR quality control. Our results could add new outlooks in order to determine the complex biological role of CtsR-mediated stress response in lactic acid bacteria.
authors.affiliation: Department of Agriculture, Food and Environment Sciences, Via Napoli 25, Foggia 71122, Italy.
authors.forename: Pasquale
authors.initials: P
authors.lastname: Russo
authors.affiliation: Biology Research Center, Department of Molecular Microbiology and Infection Biology, calle Ramiro de Maetzu 9, Madrid 28040, Spain.
authors.forename: María
authors.initials: M
authors.lastname: De la Luz Mohedano
authors.affiliation: Department of Agriculture, Food and Environment Sciences, Via Napoli 25, Foggia 71122, Italy.
authors.forename: Vittorio
authors.initials: V
authors.lastname: Capozzi
authors.affiliation: Biology Research Center, Department of Molecular Microbiology and Infection Biology, calle Ramiro de Maetzu 9, Madrid 28040, Spain.
authors.forename: Pilar Fernández
authors.initials: PF
authors.lastname: De Palencia
authors.affiliation: Biology Research Center, Department of Molecular Microbiology and Infection Biology, calle Ramiro de Maetzu 9, Madrid 28040, Spain.
authors.forename: Paloma
authors.identifier: 0000-0001-8755-8952
authors.initials: P
authors.lastname: López
authors.affiliation: Department of Agriculture, Food and Environment Sciences, Via Napoli 25, Foggia 71122, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Spano
authors.affiliation: Department of Clinical and Experimental Medicine, University of Foggia, Via Pinto, 1, Foggia 71122, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Fiocco
mesh_terms: D000251:Adenosine Triphosphatases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D006360:Heat-Shock Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D018869:Heat-Shock Response; D006358:Hot Temperature; D048191:Lactiplantibacillus plantarum / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D040901:Proteomics; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C118338:CtsR protein, bacteria; D006360:Heat-Shock Proteins; D012097:Repressor Proteins; D000251:Adenosine Triphosphatases; C118340:ClpE protein, bacteria
keywords: ClpE; CtsR; Lactobacillus plantarum; heat; stress