title: Heme oxygenase-1 is involved in nitric oxide- and cGMP-induced α-Amy2/54 gene expression in GA-treated wheat aleurone layers.
abstract: Here, α-Amy2/54 gene expression was used as a molecular probe to investigate the interrelationship among nitric oxide (NO), cyclic GMP (cGMP), and heme oxygenase-1 (HO-1) in GA-treated wheat aleurone layers. The inducible expressions of α-Amy2/54 and α-amylase activity were respectively amplified by two NO-releasing compounds, sodium nitroprusside (SNP) and spermine NONOate, in a GA-dependent fashion. Similar responses were observed when an inducer of HO-1, hemin-or one of its catalytic products, carbon monoxide (CO) in aqueous solution-was respectively added. The SNP-induced responses, mimicked by 8-bromoguanosine 3',5'-cyclic monophosphate (8-Br-cGMP), a cGMP derivative, were NO-dependent. This conclusion was supported by the fact that endogenous NO overproduction was rapidly induced by SNP, and thereafter induction of α-Amy2/54 gene expression and increased α-amylase activity were sensitive to the NO scavenger. We further observed that the above induction triggered by SNP and 8-Br-cGMP was partially prevented by zinc protoporphyrin IX (ZnPPIX), an inhibitor of HO-1. These blocking effects were clearly reversed by CO, confirming that the above response was HO-1-specific. Further analyses showed that both SNP and 8-Br-cGMP rapidly up-regulated HO-1 gene expression and increased HO activity, and SNP responses were sensitive to cPTIO and the guanylate cyclase inhibitor 6-anilino-5,8-quinolinedione (LY83583). Molecular evidence confirmed that GA-induced GAMYB and ABA-triggered PKABA1 transcripts were up-regulated or down-regulated by SNP, 8-Br-cGMP or CO cotreated with GA. Contrasting changes were observed when cPTIO, LY83583, or ZnPPIX was added. Together, our results suggested that HO-1 is involved in NO- and cGMP-induced α-Amy2/54 gene expression in GA-treated aleurone layers.
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Wu
authors.forename: Fangquan
authors.initials: F
authors.lastname: Wang
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Xie
authors.forename: Bin
authors.initials: B
authors.lastname: Han
authors.forename: Jingjing
authors.initials: J
authors.lastname: Huang
authors.forename: Wenbiao
authors.initials: W
authors.lastname: Shen
mesh_terms: D000634:Aminoquinolines / Q000494:pharmacology; D001565:Benzoates / Q000494:pharmacology; D002248:Carbon Monoxide / Q000494:pharmacology; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D017343:Genes, Plant / Q000187:drug effects; D005875:Gibberellins / Q000494:pharmacology; D006162:Guanylate Cyclase / Q000037:antagonists & inhibitors; D051547:Heme Oxygenase-1 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D007093:Imidazoles / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism / Q000494:pharmacology; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D011524:Protoporphyrins / Q000494:pharmacology; D015398:Signal Transduction; D013096:Spermine / Q000031:analogs & derivatives / Q000494:pharmacology; D014908:Triticum / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D000516:alpha-Amylases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000634:Aminoquinolines; D001565:Benzoates; D004791:Enzyme Inhibitors; D005875:Gibberellins; D007093:Imidazoles; D020030:Nitric Oxide Donors; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011524:Protoporphyrins; C091861:spermine nitric oxide complex; C079393:1,3-dihydroxy-4,4,5,5-tetramethyl-2-(4-carboxyphenyl)tetrahydroimidazole; C017803:zinc protoporphyrin; D009599:Nitroprusside; D013096:Spermine; C016276:8-bromocyclic GMP; D009569:Nitric Oxide; D002248:Carbon Monoxide; C041715:6-anilino-5,8-quinolinedione; C007842:gibberellic acid; D051547:Heme Oxygenase-1; D000516:alpha-Amylases; D006162:Guanylate Cyclase; D006152:Cyclic GMP