title: Development of a peptide nucleic acid probe to Trichosporon species and identification of trichosporonosis by use of in situ hybridization in formalin-fixed and paraffin-embedded (FFPE) sections.
abstract: In order to identify Trichosporon species in formalin-fixed and paraffin-embedded sections from which visual discrimination of non-glabrata Candida species is mostly ineffective but critical for the choice of antifungals, we tested the usefulness of a newly designed peptide nucleic acid probe (PNA) for in situ hybridization (ISH). Results confirmed the usefulness of ISH with our PNA probe in identifying Trichosporon species from Candida albicans.
authors.affiliation: Department of Surgical Pathology, Toho University School of Medicine, Tokyo, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Shinozaki
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Okubo
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sasai
authors.forename: Haruo
authors.initials: H
authors.lastname: Nakayama
authors.forename: Somay Yamagata
authors.initials: SY
authors.lastname: Murayama
authors.forename: Tadashi
authors.initials: T
authors.lastname: Ide
authors.forename: Megumi
authors.initials: M
authors.lastname: Wakayama
authors.forename: Takao
authors.initials: T
authors.lastname: Ishiwatari
authors.forename: Naobumi
authors.initials: N
authors.lastname: Tochigi
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nemoto
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Shibuya
mesh_terms: D002176:Candida albicans / Q000235:genetics; D005557:Formaldehyde / Q000378:metabolism; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics; D016612:Paraffin Embedding; D057089:Pathology, Molecular / Q000379:methods*; D020135:Peptide Nucleic Acids / Q000235:genetics; D016707:Tissue Fixation; D014250:Trichosporon / Q000235:genetics / Q000302:isolation & purification*; D060586:Trichosporonosis / Q000175:diagnosis*
chemical_list: D015345:Oligonucleotide Probes; D020135:Peptide Nucleic Acids; D005557:Formaldehyde