title: Meiotic prophase requires proteolysis of M phase regulators mediated by the meiosis-specific APC/CAma1.
abstract: Whereas proliferating cells enter M phase shortly after DNA replication, the first M phase of meiosis is preceded by an extended prophase in which homologous chromosomes undergo recombination. Exit from prophase I is controlled by the recombination checkpoint (RC), which, in yeast, represses the meiosis-specific transcription factor Ndt80 required for the expression of B-type cyclins and other M phase regulators. We show that an extended prophase I additionally requires the suppression of latent, mitotic cell-cycle controls by the anaphase-promoting complex (APC/C) and its meiosis-specific activator Ama1, which trigger the degradation of M phase regulators and Ndd1, a subunit of a mitotic transcription factor. ama1Δ mutants exit from prophase I prematurely and independently of the RC, which results in recombination defects and chromosome missegregation. Thus, control of prophase I by meiotic mechanisms depends on the suppression of the alternative, mitotic mechanisms by a meiosis-specific form of the APC/C.
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
authors.forename: Elwy
authors.initials: E
authors.lastname: Okaz
authors.forename: Orlando
authors.initials: O
authors.lastname: Argüello-Miranda
authors.forename: Aliona
authors.initials: A
authors.lastname: Bogdanova
authors.forename: P K
authors.initials: PK
authors.lastname: Vinod
authors.forename: Jesse J
authors.initials: JJ
authors.lastname: Lipp
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Markova
authors.forename: Ievgeniia
authors.initials: I
authors.lastname: Zagoriy
authors.forename: Bela
authors.initials: B
authors.lastname: Novak
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Zachariae
mesh_terms: D064173:Anaphase-Promoting Complex-Cyclosome; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins / Q000378:metabolism*; D020090:Chromosome Segregation; D015825:Chromosomes, Fungal / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D008540:Meiosis; D008677:Metaphase; D011418:Prophase; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D059748:Proteolysis; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D008941:Spindle Apparatus; D014157:Transcription Factors / Q000378:metabolism; D043743:Ubiquitin-Protein Ligase Complexes / Q000378:metabolism*
chemical_list: C577515:Ama1 protein, S cerevisiae; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C118820:NDD1 protein, S cerevisiae; C097071:NDT80 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome; D017346:Protein Serine-Threonine Kinases; C081927:CDC5 protein, S cerevisiae