title: [Removal of αGal xenotransplantation antigen by a novel α-galactosidase].
abstract: αGal, a xenotransplantations antigen (XTA), can lead to hyper acute reaction (HAR) in xenotransplantation. α-Galactosidase from B. fragilis is a novel galactosidase belong to CAZy GH110 which can clear the terminal αGal from branched and linear oligosaccharides. This study was purposed to investigate the removal effect of a novel α-galactosidase on α-Gal XTA on surface of red blood cells. The αGal XTA from the red blood cells of cattle, pig, dog and rabbit was digested by using recombinant α-galactosidase; the α-Gal antigens on surface of cells was detected by flow cytometry. The results showed that the XTA was disappeared completely or mainly. It is concluded that the novel α-galactosidase is a potential enzyme to remove the XTA on the surface of xenotransplants and can be used to overcome the HAR in xenotransplantation.
authors.affiliation: Department of Blood Molecular Biology, Beijing Institute of Transfusion Medicine, Beijing, China.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Gao
authors.forename: Xue
authors.initials: X
authors.lastname: Zhang
authors.forename: Su-Bo
authors.initials: SB
authors.lastname: Li
authors.forename: Ying-Xia
authors.initials: YX
authors.lastname: Tan
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Bao
authors.forename: Ying-Li
authors.initials: YL
authors.lastname: Wang
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Xu
authors.forename: Shou-Ping
authors.initials: SP
authors.lastname: Ji
authors.forename: Feng
authors.initials: F
authors.lastname: Gong
mesh_terms: D000818:Animals; D015478:Antigens, Heterophile / Q000276:immunology*; D002417:Cattle; D004285:Dogs; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology*; D008253:Macaca mulatta; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D013552:Swine; D014183:Transplantation, Heterologous; D000519:alpha-Galactosidase / Q000276:immunology*
chemical_list: D015478:Antigens, Heterophile; D000939:Epitopes; D000519:alpha-Galactosidase