title: Isolation and culture of alveolar epithelial Type I and Type II cells from rat lungs.
abstract: The pulmonary alveolar epithelium, comprised of alveolar Type I (TI) and Type II (TII) cells, covers more than 99% of the internal surface area of the lungs. The study of isolated and cultured alveolar epithelial TI and TII cells has provided a large amount of information about the functions of both cell types. This chapter provides information about methods for isolating and culturing both of these cell types from rat lungs.
authors.affiliation: The Cardiovascular Research Institute, University of California, San Francisco, CA, USA. Robert.gonzalez@ucsf.edu
authors.forename: Robert F
authors.initials: RF
authors.lastname: Gonzalez
authors.forename: Leland G
authors.initials: LG
authors.lastname: Dobbs
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D004847:Epithelial Cells / Q000166:cytology*; D005434:Flow Cytometry / Q000379:methods*; D059346:Magnets; D010196:Pancreatic Elastase / Q000378:metabolism; D010477:Perfusion; D011650:Pulmonary Alveoli / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D010196:Pancreatic Elastase