title: Pharmacokinetics of ginkgolide B injection in beagle dogs.
abstract: A liquid chromatography-mass spectrometry method was developed, validated, and applied to the pharmacokinetic study with doses of 0.68, 2.73 and 10.92 mg/kg of ginkgolide B in beagle dogs after intravenous infusion.An aliquot of blood samples were -collected, separated and quantitatively analyzed by liquid chromatography-mass spectrometry method with mobile phase of acetonitrile-0.02% ammonia solution (33:67, v/v) at a flow rate of 0.8 mL/min on the UltimateTM XB-C18 column (5 μm, 4.6×150 mm).The method was sensitive, accurate and convenient, and can be used for the determination of ginkgolide B in beagle dogs. The Cmax and AUC0-∞ of GB increased with dose escalation, but ANOVA analyses showed that no significant difference was observed in other pharmacokinetic parameters between different doses.An LC/MS method was developed with good sensitivity, reproducibility and specificity. In the pharmacokinetic study of GB in beagle dogs, linear pharmacokinetics was found at doses from 0.62 to 10.92 mg/kg after a single-dose intravenous infusion. Gender differences were not observed in the pharmacokinetics of GB.
authors.affiliation: Institute of Clinical Pharmacology, Qilu Hospital of Shandong University, People's Republic of China.
authors.forename: H
authors.initials: H
authors.lastname: Song
authors.forename: F
authors.initials: F
authors.lastname: Bu
authors.forename: C
authors.initials: C
authors.lastname: Wei
authors.forename: G
authors.initials: G
authors.lastname: Yuan
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: B
authors.initials: B
authors.lastname: Wang
authors.forename: R
authors.initials: R
authors.lastname: Guo
mesh_terms: D000818:Animals; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D005260:Female; D046934:Ginkgolides / Q000008:administration & dosage / Q000493:pharmacokinetics*; D006207:Half-Life; D007202:Indicators and Reagents; D007262:Infusions, Intravenous; D007783:Lactones / Q000008:administration & dosage / Q000493:pharmacokinetics*; D008297:Male; D011786:Quality Control; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D046934:Ginkgolides; D007202:Indicators and Reagents; D007783:Lactones; C045856:ginkgolide B