title: Synthesis of a targeted biarsenical Cy3-Cy5 affinity probe for super-resolution fluorescence imaging.
abstract: Photoswitchable fluorescent probes capable of the targeted labeling of tagged proteins are of significant interest due to their ability to enable in situ imaging of protein complexes within native biomolecular assemblies. Here we describe the synthesis of a fluorescent probe (AsCy3Cy5) and demonstrate the targeted labeling and super-resolution imaging of a tagged protein within a supramolecular protein complex.
authors.affiliation: Biological Sciences Division, Fundamental Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington 99352, United States.
authors.forename: Na
authors.initials: N
authors.lastname: Fu
authors.forename: Yijia
authors.initials: Y
authors.lastname: Xiong
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Squier
mesh_terms: D001152:Arsenicals / Q000737:chemistry*; D002232:Carbocyanines / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence
chemical_list: D001152:Arsenicals; D002232:Carbocyanines; D005456:Fluorescent Dyes; C089187:cyanine dye 3; C085321:cyanine dye 5