title: Antimicrobial efficacy of cryotreatment against Enterococcus faecalis in root canals.
abstract: The purpose of this investigation was to evaluate intracanal bacterial reduction by cryotreatment using a dental instrument equipped with a duct and connected to a cryogenic fluid source. A total of 86 roots were infected with Enterococcus faecalis and incubated. After incubation, the contaminated roots were divided into three study groups: 35 roots irrigated with 2 ml of a 5% sodium hypochlorite (NaOCl) solution, 35 roots irrigated with 2 ml of a 5% NaOCl solution and further treated with cryo and 10 roots irrigated with 2 ml of saline solution, plus positive and negative controls. Subsequent to each irrigation treatment, the residual bacterial colonies were counted. The use of cryo-instrumentation in association with NaOCl irrigation significantly reduced the number of Ent. faecalis (P < 0·01) in the root canal compared with controls. The interesting potential of cryotreatment should be further investigated through clinical studies aimed to establish a correct irrigation protocol. Within the limits of the study, the cryotreatment seems to have a greater effect on the reduction in bacteria compared to a standard NaOCl irrigation.
authors.affiliation: Department of Public Health and Microbiology, University of Turin, Turin, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Mandras
authors.forename: V
authors.initials: V
authors.lastname: Allizond
authors.forename: A
authors.initials: A
authors.lastname: Bianco
authors.forename: G
authors.initials: G
authors.lastname: Banche
authors.forename: J
authors.initials: J
authors.lastname: Roana
authors.forename: L
authors.initials: L
authors.lastname: Piazza
authors.forename: P
authors.initials: P
authors.lastname: Viale
authors.forename: A M
authors.initials: AM
authors.lastname: Cuffini
mesh_terms: D000890:Anti-Infective Agents / Q000627:therapeutic use; D015169:Colony Count, Microbial; D017679:Cryotherapy / Q000295:instrumentation / Q000379:methods; D003762:Dental Instruments; D003786:Dental Pulp Cavity / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000302:isolation & purification*; D006801:Humans; D012388:Root Canal Irrigants / Q000494:pharmacology*; D012390:Root Canal Therapy; D012965:Sodium Chloride / Q000008:administration & dosage; D012973:Sodium Hypochlorite / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D012388:Root Canal Irrigants; D012965:Sodium Chloride; D012973:Sodium Hypochlorite