title: A novel and effective human hepatocyte growth factor kringle 1 domain inhibits ocular neovascularization.
abstract: Neovascularization is the critical pathological process and the leading cause of blindness in a variety of clinical conditions. This angiogenesis process is still uncertain. Human hepatocyte growth factor 1 (HGFK1) is derived from the mature form of hepatocyte growth factor, which contains four kringle domains in its α-chain. This study aimed to investigate the antiangiogenic activity of HGFK1 using in vitro and in vivo assays. HGFK1 was added into the DMEM to test the proliferation and migration of human umbilical vascular endothelial cells (HUVEC), and it was intravitreously injected in laser photocoagulation-induced choroidal neovascularization (NV) model, oxygen-induced retinopathy model and rho/VEGF transgenic mice to test its antiangiogenic effect. The results showed that HGFK1 effectively inhibited VEGF-stimulated HUVEC proliferation and migration, and also had anti-NV activity in choroidal NV and retinal NV. It is suggested that HGFK1 has antiangiogenic activity in vitro and in vivo. It may lead to new potential drug discoveries and the development in addition to anti-VEGF therapy in the future clinical anti-angiogenesis treatment.
authors.affiliation: The Department of Ophthalmology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin Er Road, Shanghai 200025, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Lu
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Xi
authors.initials: X
authors.lastname: Shen
authors.forename: Yanji
authors.initials: Y
authors.lastname: Zhu
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Zhou
authors.forename: Renbao
authors.initials: R
authors.lastname: Gan
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Yisheng
authors.initials: Y
authors.lastname: Zhong
authors.forename: Bing
authors.initials: B
authors.lastname: Xie
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020256:Choroidal Neovascularization / Q000473:pathology / Q000517:prevention & control*; D004195:Disease Models, Animal; D005260:Female; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects; D006801:Humans; D058449:Intravitreal Injections; D018082:Kringles; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D015861:Retinal Neovascularization / Q000473:pathology / Q000517:prevention & control*; D042461:Vascular Endothelial Growth Factor A / Q000633:toxicity
chemical_list: D020533:Angiogenesis Inhibitors; C066855:HGF protein, human; D042461:Vascular Endothelial Growth Factor A; D017228:Hepatocyte Growth Factor