title: [Determination of maternal and foetal serum lipid profile and placental oxidised low density lipoprotein accumulation in preeclampsia and normotensive pregnancies].
abstract: BACKGROUND
  Oxidised low density lipoproteins (oxLDL) are key players in the development of atherosclerotic cardiovascular diseases. Since there are similarities between the pathogenesis of preeclampsia and atherosclerosis we hypothesised an increased accumulation of oxLDL at the materno-foetal and foeto-foetal interface within the placental tissue of preeclamptic women compared to women with normotensive pregnancies (controls). Moreover, we analysed maternal and foetal serum lipid parameters.
  
  
  METHODS
  oxLDL was determined by immunohistochemistry in placental paraffin sections of 14 women suffering from preeclampsia (30th-39th week of gestation) and compared to 28 preterm and term deliveries (25th-40th week of gestation). 10 high power fields were chosen randomly by the newCAST software and oxLDL expression was analysed via standardised methods by 2 independent and blinded investigators. Maternal and foetal triglycerides, total cholesterol, LDL cholesterol and HDL cholesterol were measured. Statistical examination was carried out by the Mann-Whitney test.
  
  
  RESULTS
  oxLDL was found in villous trophoblast and placental endothelium. No significant differences were observed in expression intensity between preeclampsia and controls. Maternal and foetal triglyceride levels were significantly increased in preeclampsia compared to controls (pre-eclampsia mothers: 293 [SD 87.4] mg/dL, controls: 214 [SD 89.4] mg/dL, p=0.0097; preeclampsia foetuses: 26 [SD 16.6] mg/dL, controls: 18 [SD 10.4] mg/dL, p=0.0463). No significant differences in other lipid concentrations were found.
  
  
  CONCLUSIONS
  We could not confirm our initial hypothesis of an increased oxLDL accumulation in placental tissue of preeclampsia. However, preeclampsia is a condition of dyslipidaemia affecting both maternal and foetal serum with implications for development and programming of cardiovascular diseases in later life.
authors.affiliation: Frauenklinik für Gynäkologie und Geburtsmedizin des Universitätsklinikums der RWTH Aachen. upecks@ukaachen.de
authors.forename: U
authors.initials: U
authors.lastname: Pecks
authors.forename: R
authors.initials: R
authors.lastname: Caspers
authors.forename: K
authors.initials: K
authors.lastname: Sosnowsky
authors.forename: N
authors.initials: N
authors.lastname: Freerksen
authors.forename: N
authors.initials: N
authors.lastname: Maass
authors.forename: B
authors.initials: B
authors.lastname: Huppertz
authors.forename: W
authors.initials: W
authors.lastname: Rath
mesh_terms: D000328:Adult; D015415:Biomarkers; D005260:Female; D005312:Fetal Blood / Q000378:metabolism*; D006801:Humans; D008055:Lipids / Q000097:blood*; D008077:Lipoproteins, LDL / Q000097:blood*; D008431:Maternal-Fetal Exchange; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; D008055:Lipids; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein