title: Escherichia coli glutathionylspermidine synthetase/amidase: phylogeny and effect on regulation of gene expression.
abstract: Glutathionylspermidine synthetase/amidase (Gss) and the encoding gene (gss) have only been studied in Escherichia coli and several members of the Kinetoplastida phyla. In the present article, we have studied the phylogenetic distribution of Gss and have found that Gss sequences are largely limited to certain bacteria and Kinetoplastids and are absent in a variety of invertebrate and vertebrate species, Archea, plants, and some Eubacteria. It is striking that almost all of the 75 Enterobacteria species that have been sequenced contain sequences with very high degree of homology to the E. coli Gss protein. To find out the physiological significance of glutathionylspermidine in E. coli, we have performed global transcriptome analyses. The microarray studies comparing gss(+)  and Δgss strains of E. coli show that a large number of genes are either up-regulated (76 genes more than threefold) or down-regulated (35 genes more than threefold) by the loss of the gss gene. Most significant categories of up-regulated genes include sulfur utilization, glutamine and succinate metabolism, polyamine and arginine metabolism, and purine and pyrimidine metabolism.
authors.affiliation: Laboratory of Biochemistry and Genetics, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA. manasc@intra.niddk.nih.gov
authors.forename: Manas K
authors.initials: MK
authors.lastname: Chattopadhyay
authors.forename: Weiping
authors.initials: W
authors.lastname: Chen
authors.forename: Herbert
authors.initials: H
authors.lastname: Tabor
mesh_terms: D019732:Amide Synthases / Q000235:genetics*; D000581:Amidohydrolases / Q000235:genetics*; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D010802:Phylogeny; D013095:Spermidine / Q000032:analysis
chemical_list: D000581:Amidohydrolases; C019665:amidase; D019732:Amide Synthases; C081536:glutathionylspermidine synthetase; D013095:Spermidine