title: A fast and sensitive quantitative lateral flow immunoassay for Cry1Ab based on a novel signal amplification conjugate.
abstract: A novel lateral flow immunoassay (LFIA) signal amplification strategy for the detection of Cry1Ab based on amplification via a polylysine (PL) chain and biotin-streptavidin system (BSAS) is described. In this system, multiple fluorescence dyes (FL) were directly coated on the surface of PL and conjugated with antibody via the BSAS for construction of novel signal amplification (FLPL-BSAS-mAb1) conjugates, in which FL, PL and BSAS were employed to improve the sensitivity of LFIA. Compared with conventional LFIA, the sensitivity of FLPL-BSAS-mAb1-based LFIA was increased by approximately 100-fold. Quantified linearity was achieved in the value range of 0-1,000 pg/mL. The limit of detection (LOD) was reached 10 pg/mL after optimization of reaction conditions. To our knowledge, this represents one of the most sensitive LFIA for Cry1Ab yet reported. Furthermore, the detection time for this method was about 10 min. Therefore, it should be an attractive alternative compared to conventional immunoassays in routine control for Cry1Ab.
authors.affiliation: Department of Control Science and Engineering, Zhejiang University, Hangzhou 310058, China. daocaoren198011@163.com
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
mesh_terms: D000906:Antibodies / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D011107:Polylysine / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D005456:Fluorescent Dyes; D011107:Polylysine; D001710:Biotin
keywords: cry1Ab; fluorescence dye; lateral flow biosensor; polylysine