title: Aluminium induced structural, metabolic alterations and protective effects of desferrioxamine in the brain tissue of mice: an FTIR study.
abstract: In this study, we intended to made a new approach to evaluate aluminium induced metabolic changes in mice brain tissue using Fourier transform infrared spectroscopy. Results demonstrate that FTIR can successfully indicate the molecular changes that occur in all groups. The overall findings demonstrate the alterations on the major biochemical constituents, such as lipids, proteins and nucleic acids of the brain tissues of mice. The significant decrease in the area value of amide A peak and Olefinic = CH stretching band suggests an alteration in the protein profile and lipid levels due to aluminium exposure, respectively. The significant shift in the amide I and amide II protein peaks may indicate the progression of aluminium induced Alzheimer's disease. Further the administration of DFO significantly improved the level of protein and brought back the amide I and II peaks nearer to the control value. Histopathological results also revealed impairment of Aluminium induced alterations in brain tissue. The results of the FTIR study were found to be in agreement with biochemical studies.
authors.affiliation: Department of Physics, Annamalai University, Annamalai Nagar, Tamilnadu 608 002, India. girihari777@yahoo.com
authors.forename: S
authors.initials: S
authors.lastname: Sivakumar
authors.forename: J
authors.initials: J
authors.lastname: Sivasubramanian
authors.forename: B
authors.initials: B
authors.lastname: Raja
mesh_terms: D000535:Aluminum / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D002614:Chelating Agents / Q000494:pharmacology*; D003676:Deferoxamine / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
chemical_list: D002614:Chelating Agents; D000535:Aluminum; D003676:Deferoxamine