title: A therapeutic vaccine using Salmonella-modified tumor cells combined with interleukin-2 induces enhanced antitumor immunity in B-cell lymphoma.
abstract: Therapeutic vaccination holds potential as complementary treatment for non-Hodgkin's lymphoma (NHL). B-NHL cells are antigen-presenting cells, but they cannot elicit proper antitumor responses because they lack expression of co-stimulatory molecules. Here, we report a novel approach to design improved whole tumor cell vaccines for B-NHL. We demonstrated that Salmonella infection significantly up-regulates CD80, CD86, CD40 and MHC II expression in lymphoma cells, and that therapeutic vaccination with infected and then irradiated lymphoma cells combined with IL-2 elicits strong anti-tumor specific immunity and extended survival in lymphoma-bearing mice. This may represent the basis of an effective immunotherapy against B-NHL that could be easily translated into the clinics.
authors.affiliation: Laboratory for Vaccine Research, Departamento de Desarrollo Biotecnologico, Instituto de Higiene, School of Medicine, Universidad de la República, Montevideo, Uruguay.
authors.forename: Sofía
authors.initials: S
authors.lastname: Grille
authors.forename: María
authors.initials: M
authors.lastname: Moreno
authors.forename: Andreina
authors.initials: A
authors.lastname: Brugnini
authors.forename: Daniela
authors.initials: D
authors.lastname: Lens
authors.forename: Jose A
authors.initials: JA
authors.lastname: Chabalgoity
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000737:chemistry / Q000627:therapeutic use*; D002478:Cells, Cultured; D003131:Combined Modality Therapy; D005260:Female; D006801:Humans; D007110:Immunity, Active / Q000187:drug effects*; D007376:Interleukin-2 / Q000494:pharmacology / Q000627:therapeutic use*; D016246:Lymphocytes, Tumor-Infiltrating / Q000187:drug effects / Q000276:immunology; D016393:Lymphoma, B-Cell / Q000150:complications / Q000276:immunology / Q000473:pathology* / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D012475:Salmonella / Q000276:immunology; D012480:Salmonella Infections / Q000150:complications / Q000276:immunology / Q000473:pathology*; D015854:Up-Regulation / Q000187:drug effects / Q000276:immunology
chemical_list: D019496:Cancer Vaccines; D007376:Interleukin-2