title: Specific changes in c-fos expression and colocalization with DNA in identified neuronal nuclei of edible snail following neurotransmitter application.
abstract: The effects of serotonin and glutamate on c-fos expression and c-Fos colocalization with DNA were immunohistochemically studied in defense behavior command neurons R-LPa2-3 in snail Helix lucorum. Simultaneous neurotransmitter application resulted in increased c-Fos-immunoreactivity and colocalization with DNA-specific stain Hoechst 33342 in LPa2 and RPa2 neurons with specific dynamics for each identified cell. In the nuclei of LPa3 and RPa3 neurons, neurotransmitter application did not significant change the c-Fos level. These findings are indicative of specific spatiotemporal changes in c-fos expression and c-Fos colocalization with DNA in investigated neurons of edible snail under the influence of neurotransmitters.
authors.affiliation: PK Anokhin Institute of Normal Physiology, Russian Academy of Medical Sciences, National Research Center Kurchatov Institute, Moscow, Russia. shevelkin@gmail.com
authors.forename: A V
authors.initials: AV
authors.lastname: Shevelkin
authors.forename: O I
authors.initials: OI
authors.lastname: Efimova
authors.forename: V P
authors.initials: VP
authors.lastname: Nikitin
authors.forename: K V
authors.initials: KV
authors.lastname: Anokhin
authors.forename: V V
authors.initials: VV
authors.lastname: Sherstnev
mesh_terms: D000818:Animals; D001562:Benzimidazoles; D002467:Cell Nucleus / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D018698:Glutamic Acid / Q000494:pharmacology; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D012701:Serotonin / Q000494:pharmacology; D012908:Snails
chemical_list: D001562:Benzimidazoles; D018377:Neurotransmitter Agents; D016760:Proto-Oncogene Proteins c-fos; D012701:Serotonin; D018698:Glutamic Acid; D004247:DNA; C017807:bisbenzimide ethoxide trihydrochloride