title: Platelet-derived growth factor induced alpha-smooth muscle actin expression by human retinal pigment epithelium cell.
abstract: OBJECTIVE
  (1) To evaluate the association between expression of α-smooth muscle actin (α-SMA) in proliferative vitreoretinopathy (PVR) and the pathological grading of PVR, and the effect of platelet-derived growth factor (PDGF) on the expression of α-SMA by human retinal pigment epithelial (RPE) cells. (2) To investigate the potential induction of PDGF on the proliferation and migration of human RPE cells as well as the signaling pathways responsible.
  
  
  METHODS
  We immunohistochemically investigated the expression of α-SMA in PVR. To further investigate the effect of PDGF and the downstream signaling, exogenous PDGF-BB and signaling inhibitors were added to cultured human RPE cells. The MTT method was performed to detected cell proliferation, while cell migration was also measured.
  
  
  RESULTS
  α-SMA expression was positively correlated with the pathological grading of PVR. PDGF-BB could stimulate the proliferation and migration of cultured RPE cells through the participation of mitogen-activated protein kinase. In addition, PDGF induced α-SMA expression. The promotion of proliferate/migration and α-SMA expression by PDGF-BB was enhanced by the presence of serum.
  
  
  CONCLUSIONS
  PDGF and α-SMA are 2 potential therapeutic targets for the treatment of PVR.
authors.affiliation: Department of Ophthalmology, Hospital 309 of PLA, Beijing, People's Republic of China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Si
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Juan
authors.initials: J
authors.lastname: Guan
authors.forename: Quanhong
authors.initials: Q
authors.lastname: Han
authors.forename: Yannian
authors.initials: Y
authors.lastname: Hui
mesh_terms: D000199:Actins / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D000077214:Becaplermin; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D005786:Gene Expression Regulation; D006801:Humans; D008875:Middle Aged; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D020574:Proto-Oncogene Proteins c-sis / Q000008:administration & dosage; D055213:Retinal Pigment Epithelium / Q000166:cytology / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D018630:Vitreoretinopathy, Proliferative / Q000473:pathology*; D055815:Young Adult
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D020928:Mitogen-Activated Protein Kinases