title: Isolation of an X-factor-dependent but porphyrin-positive Escherichia coli from urine of a patient with hemorrhagic cystitis.
abstract: An Escherichia coli isolate was recovered from a 92-year-old female patient with urinary tract infection. Gram-stained preparation of the urine sediment manifested some gram-negative rod-shaped cells, and the urine specimen culture yielded nonhemolytic colonies on sheep blood agar plate. However, no visible colonies appeared on modified Drigalski agar plate. The isolate was finally identified as an X-factor-dependent E. coli. The interesting finding was that the isolate revealed a positive reaction for porphyrin test despite the requirement of hemin. This finding suggested that some pyrrol-ring-containing porphyrin compounds or fluorescent porphyrins had been produced as chemical intermediates in the synthetic pathway from δ-amino-levulinic acid (ALA), although the isolate should be devoid of synthesizing hems from ALA. This was the first clinical isolation of such a strain, indicating that the E. coli isolate should possess incomplete synthetic pathways of hems from ALA.
authors.affiliation: Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, 390-8621, Japan.
authors.forename: Takehisa
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Kawakami
authors.forename: Akane
authors.initials: A
authors.lastname: Sueki
authors.forename: Eriko
authors.initials: E
authors.lastname: Kasuga
authors.forename: Kozue
authors.initials: K
authors.lastname: Oana
authors.forename: Kazuki
authors.initials: K
authors.lastname: Horiuchi
authors.forename: Miyuki
authors.initials: M
authors.lastname: Kato
authors.forename: Takayuki
authors.initials: T
authors.lastname: Honda
mesh_terms: D000369:Aged, 80 and over; D001431:Bacteriological Techniques; D003556:Cystitis / Q000175:diagnosis / Q000382:microbiology* / Q000652:urine; D004926:Escherichia coli / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D004927:Escherichia coli Infections / Q000175:diagnosis / Q000382:microbiology* / Q000652:urine; D005260:Female; D006470:Hemorrhage / Q000382:microbiology / Q000652:urine; D006801:Humans; D014552:Urinary Tract Infections / Q000175:diagnosis / Q000382:microbiology* / Q000652:urine