title: Nuclear lipid droplets: a novel nuclear domain.
abstract: We investigated nuclear neutral-lipid (NL) composition and organization, as NL may represent an alternative source for providing fatty acids and cholesterol (C) to membranes, signaling paths, and transcription factors in the nucleus. We show here that nuclear NL were organized into nonpolar domains in the form of nuclear-lipid droplets (nLD). By fluorescent confocal microscopy, representative nLD were observed in situ within the nuclei of rat hepatocytes in vivo and HepG2 cells, maintained under standard conditions in culture, and within nuclei isolated from rat liver. nLD were resistant to Triton X-100 and became stained with Sudan Red, OsO4, and BODIPY493/503. nLD and control cytosolic-lipid droplets (cLD) were isolated from rat-liver nuclei and from homogenates, respectively, by sucrose-gradient sedimentation. Lipids were extracted, separated by thin-layer chromatography, and quantified. nLD were composed of 37% lipids and 63% proteins. The nLD lipid composition was as follows: 19% triacylglycerols (TAG), 39% cholesteryl esters, 27% C, and 15% polar lipids; whereas the cLD composition contained different proportions of these same lipid classes, in particular 91% TAG. The TAG fatty acids from both lipid droplets were enriched in oleic, linoleic, and palmitic acids. The TAG from the nLD corresponded to a small pool, whereas the TAG from the cLD constituted the main cellular pool (at about 100% yield from the total homogenate). In conclusion, nLD are a domain within the nucleus where NL are stored and organized and may be involved in nuclear lipid homeostasis.
authors.affiliation: INIBIOLP (CCT-La Plata-CONICET-UNLP), La Plata, Buenos Aires, Argentina.
authors.forename: J P
authors.initials: JP
authors.lastname: Layerenza
authors.forename: P
authors.initials: P
authors.lastname: González
authors.forename: M M
authors.initials: MM
authors.lastname: García de Bravo
authors.forename: M P
authors.initials: MP
authors.lastname: Polo
authors.forename: M S
authors.initials: MS
authors.lastname: Sisti
authors.forename: A
authors.initials: A
authors.lastname: Ves-Losada
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D051381:Rats; D017208:Rats, Wistar