title: Effects of ACE inhibition and ANG II stimulation on renal Na-Cl cotransporter distribution, phosphorylation, and membrane complex properties.
abstract: The renal distal tubule Na-Cl cotransporter (NCC) reabsorbs <10% of the filtered Na(+) but is a key control point for blood pressure regulation by angiotensin II (ANG II), angiotensin-converting enzyme inhibitors (ACEI), and thiazide diuretics. This study aimed to determine whether NCC phosphorylation (NCCp) was regulated by acute (20-30 min) treatment with the ACEI captopril (12 μg/min × 20 min) or by a sub-pressor dose of ANG II (20 ng·kg(-1)·min(-1)) in Inactin-anesthetized rats. By immuno-EM, NCCp was detected exclusively in or adjacent to apical plama membranes (APM) in controls and after ACEI or ANG II treatment, while NCC total was detected in both APM and subapical cytoplasmic vesicles (SCV) in all conditions. In renal homogenates, neither ACEI nor ANG II treatment altered NCCp abundance, assayed by immunoblot. However, by density gradient fractionation we identified a pool of low-density APM in which NCCp decreased 50% in response to captopril and was restored during ANG II infusion, and another pool of higher-density APM that responded reciprocally, indicative of regulated redistribution between two APM pools. In both pools, NCCp was preferentially localized to Triton-soluble membranes. Blue Native gel electrophoresis established that APM NCCp localized to ~700 kDa complexes (containing γ-adducin) while unphosphorylated NCC in intracellular membranes primarily localized to ~400 kDa complexes: there was no evidence for native monomeric or dimeric NCC or NCCp. In summary, this study demonstrates that phosphorylated NCC, localized to multimeric complexes in the APM, redistributes in a regulated manner within the APM in response to ACEI and ANG II.
authors.affiliation: Department of Cell and Neurobiology, Keck School of Medicine of University of Southern California, Los Angeles, CA 90033, USA.
authors.forename: Donna H
authors.initials: DH
authors.lastname: Lee
authors.forename: Arvid B
authors.initials: AB
authors.lastname: Maunsbach
authors.forename: Anne D
authors.initials: AD
authors.lastname: Riquier-Brison
authors.forename: Mien T X
authors.initials: MT
authors.lastname: Nguyen
authors.forename: Robert A
authors.initials: RA
authors.lastname: Fenton
authors.forename: Sebastian
authors.initials: S
authors.lastname: Bachmann
authors.forename: Alan S
authors.initials: AS
authors.lastname: Yu
authors.forename: Alicia A
authors.initials: AA
authors.lastname: McDonough
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D000818:Animals; D002148:Calmodulin-Binding Proteins / Q000032:analysis; D002216:Captopril / Q000494:pharmacology; D007686:Kidney Tubules, Distal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D049993:Sodium Chloride Symporter Inhibitors / Q000494:pharmacology; D049992:Sodium Chloride Symporters / Q000378:metabolism*; D000099291:Adducins
chemical_list: D000804:Angiotensin II; D000806:Angiotensin-Converting Enzyme Inhibitors; D002148:Calmodulin-Binding Proteins; D002216:Captopril; D049993:Sodium Chloride Symporter Inhibitors; D049992:Sodium Chloride Symporters; D000099291:Adducins