title: Biolistic DNA delivery to leaf tissue of plants with the non-vacuum gene gun (HandyGun).
abstract: Non-vacuum gene guns such as HandyGun are flexible tools for bombardment of targets of varying size. Construction of HandyGun is simpler and cheaper than vacuum gene guns and will be described here. The conditions for maximal transient transformation efficiency of plant cells with plasmid DNA using HandyGun will be provided.
authors.affiliation: Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland.
authors.forename: Anssi L
authors.initials: AL
authors.lastname: Vuorinen
authors.forename: Arto
authors.initials: A
authors.lastname: Nieminen
authors.forename: Victor
authors.initials: V
authors.lastname: Gaba
authors.forename: Sidona
authors.initials: S
authors.lastname: Sikorskaite
authors.forename: Jari P T
authors.initials: JP
authors.lastname: Valkonen
mesh_terms: D019470:Biolistics / Q000295:instrumentation*; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics*; D018515:Plant Leaves / Q000166:cytology* / Q000235:genetics*
chemical_list: D004247:DNA