title: Bioengineered protein phosphatase 2A: update on need.
abstract: Harmful algal blooms caused by phytoplankton can occur in all aquatic environments. Some of the algae present in these blooms are capable of producing extremely potent toxins. Due to climate change and eutrophication, harmful algal blooms are increasing on a global scale. One kind of toxin producing algae are those that produce okadaic acid, its derivatives (dinophysistoxin-1 and 2), and microcystins. These toxins are potent inhibitors of protein phosphatase 2A, so this protein is used to detect the mentioned toxins in natural samples. Originally protein phosphatase 2A purified from animal tissues was used, but enzyme activity and stability fluctuations prevented the use of the enzyme in detection kits. Expression of the enzyme as a recombinant protein provided a solution to this problem. For this purpose, several strategies have been followed. We evaluated the activity, specificity and stability of the human protein phosphatase 2A catalytic subunit α expressed in insect larvae and showed that this expression system can be a reliable source of high quantities of stable enzyme.
authors.forename: Juan A
authors.initials: JA
authors.lastname: Rubiolo
authors.forename: Henar
authors.initials: H
authors.lastname: López-Alonso
authors.forename: Amparo
authors.initials: A
authors.lastname: Alfonso
authors.forename: Félix V
authors.initials: FV
authors.lastname: Vega
authors.forename: Mercedes Rodríguez
authors.initials: MR
authors.lastname: Vieytes
authors.forename: Luis M
authors.initials: LM
authors.lastname: Botana
keywords: eutrophication; harmful algal blooms; insect larvae; natural toxin detection; recombinant PP2AC