title: Cytokine expression and the role of Thl7 cells in mice colitis.
abstract: OBJECTIVE
  To explore the expression and mechanism of Thl7 cells and cytokines in mice model with 2,4,6-trinitrobenzenesulphonic acid (TNBS)-induced inflammatory bowel disease (IBD).
  
  
  METHODS
  ELISA assay was used to detect the expression of Th17 cytokine IL-17 and Thl cytokines IFN-y in colon tissues. Western blot assay was applied to detect IL-17 expression in peripheral blood mononuclear cells (PBMC), spleen mononuclear cells (SMC), mesenteric lymph node cells and colon tissue of colitic mice. RT-PCR assay was used to detect the effect of anti-IL-17 antibody application on TNF-a, IFN-y and IL-6 mRNA levels in SMCs from colitic mice.
  
  
  RESULTS
  Th17 cytokine IL-17 and Thl cytokines IFN-y were both expressed at high level in TNBS-induced colitic mice. In addition, the expression of Thl7 cytokine appeared earlier than the Thl cytokine. IL-17 levels in SMCs, mesenteric lymph node cells and colon tissue of the disease model group had significant differences compared with normal control group (p<0.01), while the IL-17 level in PBMCs of the disease model group had no significant difference (p>0.05) to control group. After application of 10 ug/mL anti-IL-7 antibody, the TNF-ct, IL-6 and IFN-y mRNA levels in SMCs of the model group showed no significant difference from that of no antibody group (p>0.05).
  
  
  CONCLUSIONS
  Both Th17 cells and Thl cells involved in TNBS-induced IBD and the role of Thl17 cells may be through inducing the secretion of pro-inflammatory cytokines.
authors.affiliation: Shengjing Hospital of China Medical University, Liaoning, Shenyang, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: He
authors.forename: Lian-Jie
authors.initials: LJ
authors.lastname: Lin
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Zheng
authors.forename: Yu
authors.initials: Y
authors.lastname: Jin
authors.forename: Yan
authors.initials: Y
authors.lastname: Lin
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D015153:Blotting, Western; D002478:Cells, Cultured; D003092:Colitis / Q000097:blood / Q000139:chemically induced / Q000235:genetics / Q000276:immunology*; D003106:Colon / Q000187:drug effects / Q000276:immunology*; D016207:Cytokines / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005786:Gene Expression Regulation; D018836:Inflammation Mediators / Q000097:blood / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013154:Spleen / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D058504:Th17 Cells / Q000187:drug effects / Q000276:immunology*; D013997:Time Factors; D014302:Trinitrobenzenesulfonic Acid
chemical_list: D000906:Antibodies; D016207:Cytokines; D018836:Inflammation Mediators; D012333:RNA, Messenger; D014302:Trinitrobenzenesulfonic Acid