title: Antigenic diversity of enteroviruses associated with nonpolio acute flaccid paralysis, India, 2007-2009.
abstract: Because of the broadened acute flacid paralysis (AFP) definition and enhanced surveillance, many nonpolio AFP (NP-AFP) cases have been reported in India since 2005. To determine the spectrum of nonpolio enterovirus (NPEV) serotypes associated with NP-AFP from polio-endemic and -free regions, we studied antigenic diversity of AFP-associated NPEVs. Of fecal specimens from 2,786 children with NP-AFP in 1 polio-endemic and 2 polio-free states, 823 (29.5%) were positive for NPEVs in RD cells, of which 532 (64.6%) were positive by viral protein 1 reverse transcription PCR. We identified 66 serotypes among 581 isolates, with enterovirus 71 most frequently (8.43%) detected, followed by enterovirus 13 (7.1%) and coxsackievirus B5 (5.0%). Most strains within a serotype represented new genogropups or subgenogroups. Agents for ≈35.0% and 70.0% of culture-positive and -negative cases, respectively, need to be identified. Association of human enterovirus with NP-AFP requires better assessment and understanding of health risks of NPEV infections after polio elimination.
authors.affiliation: Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India. cdr@mcbl.iisc.ernet.in
authors.forename: C Durga
authors.initials: CD
authors.lastname: Rao
authors.forename: Prasanna
authors.initials: P
authors.lastname: Yergolkar
authors.forename: K Subbanna
authors.initials: KS
authors.lastname: Shankarappa
mesh_terms: D000208:Acute Disease; D000940:Antigenic Variation / Q000276:immunology*; D002675:Child, Preschool; D004770:Enterovirus / Q000145:classification / Q000235:genetics / Q000276:immunology*; D004769:Enterovirus Infections / Q000453:epidemiology* / Q000276:immunology*; D006801:Humans; D015994:Incidence; D007194:India / Q000453:epidemiology; D007223:Infant; D008969:Molecular Sequence Data; D010243:Paralysis / Q000453:epidemiology* / Q000209:etiology*; D010802:Phylogeny; D012703:Serotyping; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D015678:Viral Structural Proteins