title: Oral administration of a nephrotoxic dose of potassium bromate, a food additive, alters renal redox and metabolic status and inhibits brush border membrane enzymes in rats.
abstract: The time dependent effect of orally administered KBrO(3) on redox status and enzymes of brush border membrane (BBM) and carbohydrate metabolism has been studied in rat kidney. Animals were given a single oral dose of KBrO(3) (100mg/kg body weight) and sacrificed at different times after this treatment; control animals were not given KBrO(3). The administration of KBrO(3) resulted in nephrotoxicity, a decline in the specific activities of several BBM marker enzymes and also induced oxidative stress in kidney. The specific activities of enzymes of carbohydrate metabolism were also altered and suggest a shift in energy metabolism from the aerobic to anaerobic mode. The renal effects of single oral dose of KBrO(3) appeared to be reversible; maximum changes in all the parameters were 48 h after administration of KBrO(3) after which recovery took place, in many cases almost to control values, after 168 h. These results suggest that the administration of a single nephrotoxic dose of KBrO(3) inhibits brush border membrane enzymes, induces oxidative stress and alters energy metabolism of the renal system in a reversible manner.
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India.
authors.forename: Mir Kaisar
authors.initials: MK
authors.lastname: Ahmad
authors.forename: Ashreeb
authors.initials: A
authors.lastname: Naqshbandi
authors.forename: Mohd
authors.initials: M
authors.lastname: Fareed
authors.forename: Riaz
authors.initials: R
authors.lastname: Mahmood
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001959:Bromates / Q000008:administration & dosage* / Q000633:toxicity; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D005503:Food Additives / Q000008:administration & dosage* / Q000633:toxicity; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008871:Microvilli / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001959:Bromates; D005503:Food Additives; C019536:potassium bromate; D008315:Malondialdehyde