title: Expression and significance of hypoxia-inducible factor-1α and survivin in laryngeal carcinoma tissue and cells.
abstract: OBJECTIVE
  The aim was to evaluate hypoxia-inducible factor-1α (HIF-1α) and survivin expression in laryngeal squamous cell carcinoma (LSCC) tissues and cell lines and to investigate whether HIF-1α has an effect on the regulation of survivin gene expression in LSCC cells under hypoxia.
  
  
  METHODS
  Prospective, observational.
  
  
  METHODS
  Shanghai Jiaotong University Affiliated First People's Hospital.
  
  
  METHODS
  The expression of HIF-1α and survivin protein in human LSCC tissues was analyzed by immunohistochemistry. HIF-1α and survivin gene expression levels in Hep-2 cells were detected by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot under normoxic or hypoxic conditions. In hypoxic cells, HIF-1α expression was inhibited by RNA interference.
  
  
  RESULTS
  HIF-1α and survivin were both highly expressed in LSCC tissues and significantly related to the clinical stage and lymph node metastasis (P < .05). Meanwhile, a positive correlation existed between HIF-1α and survivin expression (r = 0.456, P < .01). In LSCC cells, HIF-1α and survivin expression were obviously upregulated in response to hypoxia (P < .05). The downregulation of HIF-1α expression dramatically decreased survivin gene expression in hypoxic cells (P < .05).
  
  
  CONCLUSIONS
  HIF-1α could be considered as an important regulator for the upregulation of survivin gene expression induced by hypoxia in LSCC cells, and both proteins could be regarded as 2 key predictors of malignant progression and metastasis of LSCC.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Affiliated Eye and ENT Hospital of Fudan University, Shanghai, China.
authors.forename: Da-wei
authors.initials: DW
authors.lastname: Li
authors.forename: Liang
authors.initials: L
authors.lastname: Zhou
authors.forename: Bin
authors.initials: B
authors.lastname: Jin
authors.forename: Jin
authors.initials: J
authors.lastname: Xie
authors.forename: Pin
authors.initials: P
authors.lastname: Dong
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D014408:Biomarkers, Tumor / Q000032:analysis; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics* / Q000378:metabolism; D007150:Immunohistochemistry; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics* / Q000378:metabolism; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D015996:Survival Rate; D000077022:Survivin; D016610:Tissue Embedding; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
chemical_list: C107489:BIRC5 protein, human; D014408:Biomarkers, Tumor; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D051034:Inhibitor of Apoptosis Proteins; D000077022:Survivin