title: Bone repair by periodontal ligament stem cellseeded nanohydroxyapatite-chitosan scaffold.
abstract: BACKGROUND
  A nanohydroxyapatite-coated chitosan scaffold has been developed in recent years, but the effect of this composite scaffold on the viability and differentiation of periodontal ligament stem cells (PDLSCs) and bone repair is still unknown. This study explored the behavior of PDLSCs on a new nanohydroxyapatite-coated genipin-chitosan conjunction scaffold (HGCCS) in vitro as compared with an uncoated genipin-chitosan framework, and evaluated the effect of PDLSC-seeded HGCCS on bone repair in vivo.
  
  
  METHODS
  Human PDLSCs were cultured and identified, seeded on a HGCCS and on a genipin-chitosan framework, and assessed by scanning electron microscopy, confocal laser scanning microscopy, MTT, alkaline phosphatase activity, and quantitative real-time polymerase chain reaction at different time intervals. Moreover, PDLSC-seeded scaffolds were used in a rat calvarial defect model, and new bone formation was assessed by hematoxylin and eosin staining at 12 weeks postoperatively.
  
  
  RESULTS
  PDLSCs were clonogenic and positive for STRO-1. They had the capacity to undergo osteogenic and adipogenic differentiation in vitro. When seeded on HGCCS, PDLSCs exhibited significantly greater viability, alkaline phosphatase activity, and upregulated the bone-related markers, bone sialoprotein, osteopontin, and osteocalcin to a greater extent compared with PDLSCs seeded on the genipin-chitosan framework. The use of PDLSC-seeded HGCCS promoted calvarial bone repair.
  
  
  CONCLUSIONS
  This study demonstrates the potential of HGCCS combined with PDLSCs as a promising tool for bone regeneration.
authors.affiliation: Key Laboratory of Oral Biomedicine of Shandong Province, Department of Periodontology, School of Stomatology, Jinan, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Ge
authors.forename: Ning
authors.initials: N
authors.lastname: Zhao
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.forename: Meijiao
authors.initials: M
authors.lastname: Yu
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Aimei
authors.initials: A
authors.lastname: Song
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.forename: Guancong
authors.initials: G
authors.lastname: Wang
authors.forename: Pishan
authors.initials: P
authors.lastname: Yang
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000502:physiology; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D004867:Equipment Design; D006801:Humans; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010012:Osteogenesis / Q000502:physiology; D010513:Periodontal Ligament / Q000166:cytology* / Q000637:transplantation; D051381:Rats; D012887:Skull Fractures / Q000473:pathology / Q000601:surgery*; D033581:Stem Cell Transplantation / Q000379:methods*; D054457:Tissue Scaffolds; D016896:Treatment Outcome
chemical_list: D048271:Chitosan; D017886:Durapatite
keywords: chitosan; hydroxyapatite; periodontal ligament; scaffold; stem cells; tissue engineering