title: Effect of anti-siglec-f antibody and reactive oxygen species blocking on histamine release in urinary bladder of ovalbumin-treated mice.
abstract: OBJECTIVE
  Sialic acid-binding Ig-like lectin (Siglec) is an immune inhibitory receptor that plays a role in the negative regulation of the activation of immune cells. This study aimed to evaluate the effects of anti-Siglec-F on plasma and urinary histamine levels in ovalbumin (OVA)-challenged urinary bladder in mice.
  
  
  METHODS
  Thirty BALB/c mice were used. In group I (control group, n=5), mice were sensitized with OVA and challenged with saline. In group II (OVA challenge group, n=5), OVA was used for intraperitoneal sensitization and intravesical challenge. The challenged mice in group III (control immunoglobulin G [IgG] group, n=5) and those in group IV (anti-Siglec-F group, n=5) were intraperitoneally pretreated with rabbit control IgG or anti-Siglec-F antibody, respectively. In groups V (N-acetylcysteine [NAC] in OVA challenge group, n=5) and VI (control NAC only, n=5), mice were pretreated with NAC.
  
  
  RESULTS
  Urinary histamine concentrations were significantly higher 7 days after intravesical OVA challenge (P<0.01), whereas plasma histamine levels were not. Pretreatment with anti-Siglec-F antibody significantly prevented the increase in urinary histamine release (P<0.05), whereas pretreatment with the IgG antibody control did not. Also, pretreatment of the OVA challenge group with NAC did not affect the histamine concentration in either urine or plasma.
  
  
  CONCLUSIONS
  Systemic anti-Siglec-F treatment showed anti-allergic effects at least on local histamine release, particularly in the lower urinary bladder.
authors.affiliation: Department of Pharmacology, Inha University School of Medicine, BK 21 Project, Incheon, Korea.
authors.forename: Bo-Hwa
authors.initials: BH
authors.lastname: Choi
authors.forename: Gwoan-Youb
authors.initials: GY
authors.lastname: Choo
authors.forename: Ju-Hee
authors.initials: JH
authors.lastname: Kang
authors.forename: Choong-Yeol
authors.initials: CY
authors.lastname: Lee
authors.forename: Chang-Shin
authors.initials: CS
authors.lastname: Park
keywords: Histamine; Mice; Ovalbumin; Reactive oxygen species; Sialic acid binding Ig-like lectin; Urinary bladder