title: Periplasmically located α-santonin binding factor in Sphingomonas paucimobilis strain S ATCC 43388.
abstract: A marked reduction in uptake of α-santonin, accompanied by loss of ability of cells to transform the substrate, is observed on shocking Sphingomonas paucimobilis strain S ATCC 43388 cells by freeze - thaw method. The shock fluid shows a 26% quench in fluorescence at 350nm on incubation with the substrate. Addition of shock fluid to the freeze thawed cells restores both uptake as well as transformation of α-santonin to near normal.
authors.affiliation: Department of microbiology, St. Francis P.G. College for Women, Begumpet, Hyderabad, 500 016 India.
authors.forename: M Shailaja
authors.initials: MS
authors.lastname: Raj
authors.forename: I
authors.initials: I
authors.lastname: Furtado
authors.forename: S
authors.initials: S
authors.lastname: Mavinkurve
keywords: Fluorescence; Periplasmic proteins; Shock fluid; Sphingomonas