title: Bronchodilator activity of aerial parts of Polygonatum verticillatum augmented by anti-inflammatory activity: attenuation of Ca²⁺ channels and lipoxygenase.
abstract: Polygonatum verticillatum is commonly used for the treatment of asthma and inflammation. The current study was aimed to scrutinize the pharmacological profile of methanolic extract of the aerial parts (PA). Isolated tracheal preparations were used for the evaluation of bronchodilatory activity, whilst the in vivo carrageenan-induced paw oedema test and an in vitro lipoxygenase (LOX) inhibitory assay were used for the assessment of the anti-inflammatory profile of PA. When tested against carbachol and K⁺ (80 mM)-induced contractions, PA caused complete inhibition of isolated rabbit tracheal preparations in a dose-dependent mode, similar to verapamil. While elucidating possible mechanism, PA shifted the Ca²⁺ concentration-response curves to the right, analogous to that produced by verapamil, confirming a Ca²⁺ channel blocker-like activity. PA provoked profound reduction in paw oedema with a maximum protection of 60.87% at 200 mg/kg i.p. in a dose-dependent manner which was augmented by its prominent LOX inhibitory activity (IC₅₀ : 125 µg/mL). These findings authenticated its therapeutic potential in the treatment of asthmatic and inflammatory conditions.
authors.affiliation: Department of Pharmacy, University of Peshawar, Peshawar 25120, Pakistan. hkdr2006@gmail.com
authors.forename: Haroon
authors.initials: H
authors.lastname: Khan
authors.forename: Muhammad
authors.initials: M
authors.lastname: Saeed
authors.forename: Anwarul Hassan
authors.initials: AH
authors.lastname: Gilani
authors.forename: Malik Hassan
authors.initials: MH
authors.lastname: Mehmood
authors.forename: Najeeb-Ur
authors.initials: NU
authors.lastname: Rehman
authors.forename: Naveed
authors.initials: N
authors.lastname: Muhammad
authors.forename: Muzaffar
authors.initials: M
authors.lastname: Abbas
authors.forename: Ikram-Ul
authors.initials: IU
authors.lastname: Haq
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001993:Bronchodilator Agents / Q000494:pharmacology*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002217:Carbachol / Q000009:adverse effects; D002351:Carrageenan / Q000009:adverse effects; D004487:Edema / Q000188:drug therapy; D005260:Female; D066298:In Vitro Techniques; D007249:Inflammation / Q000188:drug therapy; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology*; D008297:Male; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D031384:Polygonatum / Q000737:chemistry*; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D014132:Trachea / Q000187:drug effects; D014700:Verapamil / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D001993:Bronchodilator Agents; D002121:Calcium Channel Blockers; D016859:Lipoxygenase Inhibitors; D010936:Plant Extracts; D002217:Carbachol; D002351:Carrageenan; D014700:Verapamil
keywords: Ca2+ channel blockers; Lipoxygenase; Polygonatum verticillatum; anti-inflammatory; bronchodilator