title: Peroxiredoxin I deficiency attenuates phagocytic capacity of macrophage in clearance of the red blood cells damaged by oxidative stress.
abstract: The role of peroxiredoxin (Prx) I as an erythrocyte antioxidant defense in red blood cells (RBCs) is controversial. Here we investigated the function of Prx I by using Prx I(-/-) and Prx I/II(-/-) mice. Prx I(-/-) mice exhibited a normal blood profile. However, Prx I/II(-/-) mice showed more significantly increased Heinz body formation as compared with Prx II(-/-) mice. The clearance rate of Heinz body-containing RBCs in Prx I(-/-) mice decreased significantly through the treatment of aniline hydrochloride (AH) compared with wild-type mice. Prx I deficiency decreased the phagocytic capacity of macrophage in clearing Heinz body-containing RBCs. Our data demonstrate that Prx I deficiency did not cause hemolytic anemia, but showed that further increased hemolytic anemia symptoms in Prx II(-/-) mice by attenuating phagocytic capacity of macrophage in oxidative stress damaged RBCs, suggesting a novel role of Prx I in phagocytosis of macrophage.
authors.affiliation: Aging Research Center, KRIBB, Daejeon, Korea.
authors.forename: Ying-Hao
authors.initials: YH
authors.lastname: Han
authors.forename: Taeho
authors.initials: T
authors.lastname: Kwon
authors.forename: Sun-Uk
authors.initials: SU
authors.lastname: Kim
authors.forename: Hye-Lin
authors.initials: HL
authors.lastname: Ha
authors.forename: Tae-Hoon
authors.initials: TH
authors.lastname: Lee
authors.forename: Jin-Man
authors.initials: JM
authors.lastname: Kim
authors.forename: Eun-Kyeong
authors.initials: EK
authors.lastname: Jo
authors.forename: Bo Yeon
authors.initials: BY
authors.lastname: Kim
authors.forename: Do Young
authors.initials: DY
authors.lastname: Yoon
authors.forename: Dae-Yeul
authors.initials: DY
authors.lastname: Yu
mesh_terms: D000743:Anemia, Hemolytic / Q000378:metabolism / Q000473:pathology; D000814:Aniline Compounds / Q000494:pharmacology; D000818:Animals; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006366:Heinz Bodies / Q000378:metabolism; D008264:Macrophages / Q000276:immunology / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D018384:Oxidative Stress; D054464:Peroxiredoxins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D010587:Phagocytosis
chemical_list: D000814:Aniline Compounds; D054464:Peroxiredoxins; C023650:aniline