title: Isolation and biological activities of decanal, linalool, valencene, and octanal from sweet orange oil.
abstract: Product 1 (82.25% valencene), product 2 (73.36% decanal), product 3 (78.12% octanal), and product 4 (90.61% linalool) were isolated from sweet orange oil by combined usage of molecular distillation and column chromatography. The antioxidant activity of sweet orange oil and these products was investigated using 2,2-diphenyl-1-picrylhydrazyl and reducing power assays. In this test, product 1 (82.25% valencene), product 2 (73.36% decanal), and product 4 (90.61% linalool) had antioxidant activity, but lower than sweet orange oil. The antimicrobial activity was investigated in order to evaluate their efficacy against 5 microorganisms. The results showed that sweet orange oil, product 2 (73.36% decanal), product 3 (78.12% octanal), and product 4 (90.61% linalool) had inhibitory and bactericidal effect on the test microorganisms (except Penicillium citrinum). Valencene did not show any inhibitory effect. Saccharomyces cerivisiae was more susceptible, especially to the crude sweet orange oil (minimal inhibitory concentration 6.25 μL/mL). The cytotoxicity was evaluated on Hela cells using the 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. All test samples showed significant cytotoxicity on the cell lines with IC(50) values much less than 20 μg/mL.
authors.affiliation: College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, PR China.
authors.forename: Kehai
authors.initials: K
authors.lastname: Liu
authors.forename: Qiulin
authors.initials: Q
authors.lastname: Chen
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhou
authors.forename: Xichang
authors.initials: X
authors.lastname: Wang
mesh_terms: D000080462:Acyclic Monoterpenes; D000447:Aldehydes / Q000032:analysis*; D000890:Anti-Infective Agents / Q000032:analysis / Q000494:pharmacology; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology; D001234:Aspergillus niger / Q000187:drug effects / Q000254:growth & development; D002855:Chromatography, Thin Layer; D032084:Citrus sinensis / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D006367:HeLa Cells; D006801:Humans; D008826:Microbial Sensitivity Tests; D039821:Monoterpenes / Q000032:analysis*; D009812:Odorants / Q000032:analysis; D010407:Penicillium / Q000187:drug effects / Q000254:growth & development; D010938:Plant Oils / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development; D012717:Sesquiterpenes / Q000032:analysis*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D013649:Taste
chemical_list: D000080462:Acyclic Monoterpenes; D000447:Aldehydes; D000890:Anti-Infective Agents; D000975:Antioxidants; D039821:Monoterpenes; D010938:Plant Oils; D012717:Sesquiterpenes; C021170:decanaldehyde; C506706:valencene; C087245:orange oil; C018584:linalool; C031639:caprylic aldehyde