title: IEF-based multidimensional applications in proteomics: toward higher resolution.
abstract: This review focuses on the alternative isoelectrofocusing methods and IEF-related techniques in protein analysis and characterization. The main emphasis is placed on the evaluation of the resolving power of new approaches, as well as potential advantages of IEF-related techniques for multidimensional analysis. In particular, the inverse 2D gel electrophoresis where the Mw separation stage precedes IEF mode is considered.
authors.affiliation: Department of Pathology and Anatomical Sciences, University of Missouri, Columbia, MO 65212, USA. stoyanova@health.missouri.edu
authors.forename: Alexander
authors.initials: A
authors.lastname: Stoyanov
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D007525:Isoelectric Focusing / Q000379:methods*; D040901:Proteomics / Q000379:methods*