title: Hematopoietic stem cell mobilization into the peripheral circulation in patients with chronic liver diseases.
abstract: OBJECTIVE
  The present study was aimed to investigate and compare the kinetics of bone marrow-derived hematopoietic stem cells (BMHSC) migration in the peripheral blood and liver in response to liver injury in patients with chronic liver disease (CLD).
  
  
  METHODS
  In all, 45 CLD patients staged with Child-Pugh A, B and C and 15 healthy participants were evaluated for the concentration of circulating BMHSC by a flow cytometric analysis of CD133(+) /CD34(+)  cells. In addition, homing BMHSC and hepatic progenitors were assessed by the immunohistochemical detection of CD133(+)  and OV6(+)  cells in liver biopsy specimens from Child-Pugh A and B patients.
  
  
  RESULTS
  No significant difference in the percentage of circulating CD133(+) /CD34(+)  cells was observed among all groups of patients. In liver tissues, OV6(+)  cells increased significantly in Child-Pugh B cases (P < 0.05), while CD133(+)  cells were distributed sparsely in the periportal region in Child-Pugh A and B patients. OV6(+)  cells were significantly correlated with CD34(+)  cells but not with CD133(+)  cells in Child-Pugh A and B patients (P < 0.01 and P < 0.05, respectively).
  
  
  CONCLUSIONS
  Various degrees of severity in CLD neither evoked the mobilization of BMHSC into the circulation nor triggered their homing into liver tissue, thus excluding extrahepatic stem cell-mediated repair. The recovery process seems to be dependent on proliferating endogenous liver progenitors (OV6(+)  cells).
authors.affiliation: Department of Electron Microscopy, Theodor Bilharz Research Institute, Giza, Egypt.
authors.forename: Lobna Y
authors.initials: LY
authors.lastname: Ghanem
authors.forename: Mona Mf
authors.initials: MM
authors.lastname: Nosseir
authors.forename: Abeya A
authors.initials: AA
authors.lastname: Lotfi
authors.forename: Ayat S
authors.initials: AS
authors.lastname: Mohamed
authors.forename: Refaat A
authors.initials: RA
authors.lastname: Ibrahim
authors.forename: Moutaz H
authors.initials: MH
authors.lastname: Hassanein
authors.forename: Eman
authors.initials: E
authors.lastname: Mansour
authors.forename: Manal M
authors.initials: MM
authors.lastname: Makhlouf
authors.forename: Yasser M
authors.initials: YM
authors.lastname: Fouad
authors.forename: Hisham R
authors.initials: HR
authors.lastname: El-Khayat
mesh_terms: D000071916:AC133 Antigen; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D001706:Biopsy; D002465:Cell Movement / Q000502:physiology*; D002908:Chronic Disease; D005260:Female; D005434:Flow Cytometry; D006023:Glycoproteins / Q000378:metabolism; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008107:Liver Diseases / Q000473:pathology* / Q000503:physiopathology*; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000378:metabolism; D012720:Severity of Illness Index
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides