title: Physicochemical characterization of fish protein adlayers with bacteria repelling properties.
abstract: Materials coated with aqueous fish protein extracts can reduce bacterial adhesion, but the mechanism behind the observed effect is not fully understood. In this study we explore the physicochemical properties of fish muscle protein adlayers on four substrates: gold, stainless steel, polystyrene and silicon dioxide. The aims were (i) to determine if the anti-adhesive effect is independent of the underlying substrate chemistry, (ii) to link the physicochemical properties of the adlayer to its ability to repel bacteria, and (iii) to elucidate the mechanism behind this effect. The main proteins on all surfaces were the muscle proteins troponin, tropomyosin, and myosin, and the lipid binding protein apolipoprotein. The quantity, viscoelasticity, and hydration of the protein adlayers varied greatly on the different substrates, but this variation did not affect the bacterial repelling properties. Our results imply that these proteins adsorb to all substrates and provide a steric barrier towards bacterial adhesion, potentially providing a universal antifouling solution.
authors.affiliation: Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Ny Munkegade 120, DK-8000 Aarhus C, Denmark. rikke.meyer@biology.au.dk
authors.forename: R L
authors.initials: RL
authors.lastname: Meyer
authors.forename: A
authors.initials: A
authors.lastname: Arpanaei
authors.forename: S
authors.initials: S
authors.lastname: Pillai
authors.forename: N
authors.initials: N
authors.lastname: Bernbom
authors.forename: J J
authors.initials: JJ
authors.lastname: Enghild
authors.forename: Y Y
authors.initials: YY
authors.lastname: Ng
authors.forename: L
authors.initials: L
authors.lastname: Gram
authors.forename: F
authors.initials: F
authors.lastname: Besenbacher
authors.forename: P
authors.initials: P
authors.lastname: Kingshott
mesh_terms: D000818:Animals; D001053:Apolipoproteins / Q000737:chemistry; D001422:Bacterial Adhesion / Q000187:drug effects*; D029941:Fish Proteins / Q000737:chemistry* / Q000494:pharmacology*; D009218:Myosins / Q000737:chemistry; D011551:Pseudomonas fluorescens / Q000187:drug effects / Q000502:physiology; D014335:Tropomyosin / Q000737:chemistry; D014336:Troponin / Q000737:chemistry
chemical_list: D001053:Apolipoproteins; D029941:Fish Proteins; D014335:Tropomyosin; D014336:Troponin; D009218:Myosins