title: Cellular immune response to infection by different genotypes of hepatitis C virus.
abstract: The present study aimed to estimate the cellular immune response to infection by different HCV genotypes in some Egyptian patients with chronic liver disease. HCV-RNA and HCV genotypes were conducted using type-specific primers to amplify the core region of HCV genome. The levels of IFN-gamma and TNF-alpha, PIIINP, SOD enzyme were also determined. The results showed that genotype 4 was the prevalent one (88.57%).Genotype 3 was found mixed with genotype 2 and 4 (11.42% and 34.28% respectively). Genotype 1 was absent from patients under study. No significant differences were observed in the level of IFN-gamma interferon, SOD enzyme and ALT enzyme between those genotypes. On the other hand the level of TNF-alpha was greatly increased in patients infected with mixed HCV genotypes 3, 4 compared with patients with genotype 4 and those infected with mixed genotypes 2, 3. Otherwise PIIINP was decreased significantly of the same group compared with other groups.
authors.affiliation: Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Minufiya University, Sadat City, Egypt  ; Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Minufiya University, Sadat City, Egypt P.O 22857-79.
authors.forename: Mohamed El-Shahat
authors.initials: Mel-S
authors.lastname: Ebeid
authors.forename: Kadry Abdel-Kader
authors.initials: KA
authors.lastname: El-Bakry
keywords: Cellular Immune response; HCV genotypes; IFN-gamma Interferon