title: Microsatellite primers for the neotropical epiphyte Epidendrum firmum (Orchidaceae).
abstract: OBJECTIVE
  Ten microsatellite loci were isolated and characterized for the neotropical epiphytic orchid Epidendrum firmum to examine levels of genetic diversity and genetic structure at multiple spatial scales.
  
  
  RESULTS
  We screened loci in 12-25 individuals from each of two populations in Costa Rica and identified 10 polymorphic loci. The number of alleles per locus ranged from one to 15 while observed heterozygosity for polymorphic loci ranged from 0.360 to 0.960.
  
  
  CONCLUSIONS
  Primers for these informative genetic markers will be useful for quantifying genetic diversity, spatial genetic structure, and gene flow in E. firmum.
authors.affiliation: Odum School of Ecology, University of Georgia, 140 E. Green Street, Athens, Georgia 30602 USA. tkartzinel@gmail.com
authors.forename: Tyler R
authors.initials: TR
authors.lastname: Kartzinel
authors.forename: Dorset W
authors.initials: DW
authors.lastname: Trapnell
authors.forename: Travis C
authors.initials: TC
authors.lastname: Glenn
mesh_terms: D000483:Alleles; D003364:Costa Rica; D017931:DNA Primers / Q000235:genetics*; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D029595:Orchidaceae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018744:DNA, Plant