title: Expression of Bis in the mouse gastrointestinal system.
abstract: The Bcl-2 interacting death suppressor (Bis) protein is known to be involved in a variety of pathophysiological conditions. We recently generated bis-deficient mice, which exhibited early lethality with typical nutritional deprivation status. To further investigate the molecular basis for the malnutrition phenotype of bis deficient mice, we explored Bis expression in the digestive system of normal mice. Western blot analysis and quantitative real time reverse transcription polymerase chain reaction analysis indicated that Bis expression is highest in the esophagus, followed by the stomach, colon, jejunum and ileum. Immunohistochemical data indicated that Bis expression is restricted to the stratified squamous epitheliums in the esophagus and forestomach, and was not notable in the columnar epitheliums in the stomach, small intestine and colon. In addition, strong Bis immunoreactivity was detected in the striated muscles surrounding the esophagus and smooth muscles at a lesser intensity throughout the gastrointestinal (GI) tract. Ganglionated plexuses, located in submucous layers, as well as intermuscular layers, were specifically immunoreactive for Bis. Immunofluorescence studies revealed that Bis is co-localized in glial fibrillary acidic protein-expressing enteric glial cells. Immunostaining with neuron specific esterase antibodies indicate that Bis is also present in the cell bodies of ganglions in the enteric nervous system (ENS). Our findings indicate that Bis plays a role in regulating GI functions, such as motility and absorption, through modulating signal transmission between the ENS and smooth muscles or the intestinal epitheliums.
authors.affiliation: Department of Biochemistry, The Catholic University of Korea College of Medicine, Seoul, Korea.
authors.forename: Young Dae
authors.initials: YD
authors.lastname: Lee
authors.forename: Jung-Sook
authors.initials: JS
authors.lastname: Yoon
authors.forename: Hye Hyeon
authors.initials: HH
authors.lastname: Yoon
authors.forename: Ho Joong
authors.initials: HJ
authors.lastname: Youn
authors.forename: Jin
authors.initials: J
authors.lastname: Kim
authors.forename: Jeong-Hwa
authors.initials: JH
authors.lastname: Lee
keywords: Bag3; Enteric glial cell; Enteric nervous system; Immunohistochemistry; Mouse