title: [Proliferation, differentiation and BMP-2 expression of osteoblasts cultured in the plane under suitable electrical stimulation].
abstract: OBJECTIVE
  To observe proliferation and differentiation of osteoblasts cultured in the plane on appropriate electrical stimulation, to specify whether it promote the proliferation, and observe expression of BMP-2 on electrical stimulation.
  
  
  METHODS
  Osteoblasts were extracted from the skull of rabbit offspring and cultured. Cells after the 2nd generations were cultured. In experimental group, cells had electrical stimulation, and same stimulation time and intensity were given. In control group cells had not electrical stimulation. The proliferation and differentiation were detected at different time, and BMP-2 protein expression was analyzed.
  
  
  RESULTS
  The cell morphology of experimental group in 8 days under the light microscope was observed and showed a lot of proliferation of osteoblasts, pleomorphic changes, in 6 to 8 days a small amount of Calcified spots was also observed; while in the control group, proliferation was slower. Differentiation of the experimental group was significantly, alkaline phosphatase staining and calcium nodules were positive, quantitative analysis of alkaline phosphatase increaseed significantly. Experimental group showed that BMP-2 was gradually increased by immunohistochemistry analysis.
  
  
  CONCLUSIONS
  Electrical stimulation can promote the proliferation and differentiation of osteoblasts and achieved the increasement the number of cells in short-term, intracellular staining by immunohistochemistry showed the increasement in expression of BMP-2.
authors.affiliation: Department of Orthopaedics, Hospital Affiliated to Medical College of Hannan, Haikou 570102, Hannan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Xiu-Cui
authors.initials: XC
authors.lastname: Li
authors.forename: Shao-Wen
authors.initials: SW
authors.lastname: Cheng
authors.forename: Qing-Yu
authors.initials: QY
authors.lastname: Chen
authors.forename: Lei
authors.initials: L
authors.lastname: Peng
authors.forename: Zhong-Qin
authors.initials: ZQ
authors.lastname: Lin
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Dong-Quan
authors.initials: DQ
authors.lastname: Kou
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D004558:Electric Stimulation; D005786:Gene Expression Regulation; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D011817:Rabbits
chemical_list: D055396:Bone Morphogenetic Protein 2