title: Detection and Identification of Toxoplasma gondii Type One Infection in Sheep Aborted Fetuses in Qazvin Province of Iran.
abstract: BACKGROUND
  The aim of this study was to apply the nested-PCR and bioassay methods in detection and genotyping of Toxoplasma gondii infection in provided sheep aborted fetus samples from Qazvin Province of Iran.
  
  
  METHODS
  Eighteen sheep aborted fetal samples were studied by nested-PCR-RFLP, histopathological observation and microbiological assay. Bioassay in mice was carried out by inoculating the brain samples intraperitoneally.
  
  
  RESULTS
  The results demonstrated the frequency of 66% infected sheep aborted fetal samples with T. gondii type one. Although we could not isolate any parasite from inoculated mice even after three passages, but it was confirmed histopathologically formation of cyst like bodies in prepared mice brain sections.
  
  
  CONCLUSIONS
  The results of the performed nested-PCR and formation of brain cyst in inoculated mice exhibited that T. gondii type one infection might be considered as one of the major causative agents for abortion in ewes.
authors.affiliation: Parasite Vaccine Research and Production Department, Razi Vaccine and Serum Research Institute, Karaj, Iran.
authors.forename: Gr
authors.initials: G
authors.lastname: Habibi
authors.forename: Ar
authors.initials: A
authors.lastname: Imani
authors.forename: Mr
authors.initials: M
authors.lastname: Gholami
authors.forename: Mh
authors.initials: M
authors.lastname: Hablolvarid
authors.forename: Am
authors.initials: A
authors.lastname: Behroozikhah
authors.forename: M
authors.initials: M
authors.lastname: Lotfi
authors.forename: M
authors.initials: M
authors.lastname: Kamalzade
authors.forename: E
authors.initials: E
authors.lastname: Najjar
authors.forename: K
authors.initials: K
authors.lastname: Esmaeil-Nia
authors.forename: S
authors.initials: S
authors.lastname: Bozorgi
keywords: Bioassay; Fetus; Genotyping; PCR; Sheep abortion; Toxoplasma gondii