title: Protein antigen delivery by gene gun-mediated epidermal antigen incorporation (EAI).
abstract: The gene gun technology can not only be employed for efficient transfer of gene vaccines into upper layers of the skin, but also for application of protein antigens. As a tissue rich in professional antigen presenting cells, the skin represents an attractive target for immunizations. In this chapter we present a method for delivery of the model antigen ovalbumin into the skin of mice termed epidermal antigen incorporation and describe in detail how antigen-specific proliferation in draining lymph nodes can be followed by flow cytometry.
authors.affiliation: Division of Allergy and Immunology, Department of Molecular Biology, University of Salzburg, Salzburg, Austria.
authors.forename: Sandra
authors.initials: S
authors.lastname: Scheiblhofer
authors.forename: Uwe
authors.initials: U
authors.lastname: Ritter
authors.forename: Josef
authors.initials: J
authors.lastname: Thalhamer
authors.forename: Richard
authors.initials: R
authors.lastname: Weiss
mesh_terms: D000818:Animals; D019470:Biolistics / Q000295:instrumentation*; D049109:Cell Proliferation; D004817:Epidermis / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000008:administration & dosage* / Q000276:immunology; D013194:Staining and Labeling
chemical_list: D010047:Ovalbumin; D016650:Fluorescein-5-isothiocyanate