title: Structure and immuno-stimulating activities of a new heteropolysaccharide from Lentinula edodes.
abstract: In this study, a new heteropolysaccharide, here called L2, was separated from the fruit body of Lentinula edodes. Chemical and physical analyses indicated that L2 has a molecular weight of 26 KDa and consists of glucose (87.5%), galactose (9.6%), and arabinose (2.8%), but it does not possess a triple-helical conformation. Stimulation of the production of nitric oxide (NO), tumor necrosis factor α (TNF-α), and interleukin 6 (IL-6) in RAW 264.7 cells showed that L2 has significant immune activities involving toll-like receptor 2 (TLR2). Further studies demonstrated that the activities of L2 exhibited high stability in wide range of pH from 4.0 to 10.0, when the thermal processing temperature was below 121 °C. Our findings revealed that a new heteropolysaccharide without triple-helical conformation from Lentinula edodes shows immuno-stimulating activities involving TLR2 at modest pH and thermal processing conditions, which enable it to act as an active component in foods.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan Road, Guangzhou, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Xu
authors.forename: Huidan
authors.initials: H
authors.lastname: Yan
authors.forename: Xuewu
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002460:Cell Line; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D007155:Immunologic Factors / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology; D015850:Interleukin-6 / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification / Q000494:pharmacology; D020942:Shiitake Mushrooms / Q000737:chemistry*; D051195:Toll-Like Receptor 2 / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D007155:Immunologic Factors; D015850:Interleukin-6; D011134:Polysaccharides; D051195:Toll-Like Receptor 2; D014409:Tumor Necrosis Factor-alpha