title: Expression and clinical significance of CD82/KAI1 and E-cadherin in non-small cell lung cancer.
abstract: BACKGROUND
  This study investigates the expression of CD82/KAI1 and epithelial-cadherin (E-cad) in non-small cell lung cancer (NSCLC).
  
  
  METHODS
  Tissues of resected primary NSCLC and normal lung tissue were investigated. Protein expression was detected with immunohistochemical staining. mRNA expression levels of CD82/KAI1 and E-cad were determined by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) after mRNA extraction.
  
  
  RESULTS
  The expression of CD82/KAI1 and E-cad was significantly lower in NSCLC compared to normal lung tissue (P < 0.01). CD82/KAI1 and E-cad mRNA and protein expression were found to be in close relationship with the grade of differentiation, lymph node metastasis and pathologic tumor-node-metastasis (pTNM) stages, and survival in NSCLC (P < 0.01), but no relationship was observed with gender, diameter, type, histological type and age (P > 0.05). Expression of mRNA CD82/KAI1 and E-cad were consistent with their proteins (P < 0.01), and there was a significant relationship between expression of CD82/KAI1 and E-cad. The survival rate of the CD82/KAI1-positive and CD82/KAI1-negative groups was significantly different (P < 0.01). In addition, the survival rate was significantly different between the E-cad-positive and E-cad-negative groups. pTNM stages and positive expression of CD82/KAI1 and E-cad were independent prognostic factors of NSCLC (P < 0.05).
  
  
  CONCLUSIONS
  Lower expression of CD82/KAI1 and E-cad was found in NSCLC compared to normal lung tissue. Decreased expression of CD82/KAI1 and E-cad was closely related to cellular differentiation, pTNM stages, invasion and metastasis.
authors.affiliation: Department of Pathology, First Affiliated Hospital of Bengbu Medical College, Bengbu Medical College, Bengbu City, 233000, Anhui Province, PR China.
authors.forename: W U
authors.initials: WU
authors.lastname: Shiwu
authors.forename: Yu
authors.initials: Y
authors.lastname: Lan
authors.forename: Song
authors.initials: S
authors.lastname: Wenqing
authors.forename: Zhou
authors.initials: Z
authors.lastname: Lei
authors.forename: Tao
authors.initials: T
authors.lastname: Yisheng
mesh_terms: D000328:Adult; D000368:Aged; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism* / Q000473:pathology* / Q000556:secondary; D005260:Female; D015870:Gene Expression; D006801:Humans; D051861:Kangai-1 Protein / Q000235:genetics / Q000378:metabolism*; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000378:metabolism*; D018709:Statistics, Nonparametric; D015996:Survival Rate
chemical_list: D015820:Cadherins; D051861:Kangai-1 Protein; D012333:RNA, Messenger