title: [Investigation of functional groups of Cryptococcus albidus alpha-L-rhamnosidase].
abstract: The effect of cations, anions and specific chemical reagents: 1-[3-(dimethylamino)propyl]-3-ethylcarbodimide methiodide, EDTA, o-phenantroline, dithiotreitol, L-cysteine, beta-mercaptoethanol, p-chlormercurybenzoate (p-ChMB), N-ethylmaleimide on the alpha-L-rhamnosidase activity of Cryptococcus albidus has been investigated. The essential role of Ag+ which inhibits the alpha-L-rhamnosidase activity by 72.5% was shown. Rhamnose at 1-5 mM protect the enzyme from the negative effect of Ag(+). It was expected that carboxyl group of C-terminal aminoacid and imidazole group of histidine would participate in the catalytic action of alpha-L-rhamnosidase on the basis of inhibition and kinetic analysis.
authors.forename: O V
authors.initials: OV
authors.lastname: Gudzenko
authors.forename: L D
authors.initials: LD
authors.lastname: Varbanets'
mesh_terms: D055162:Biocatalysis; D002414:Cations, Monovalent; D003454:Cryptococcus / Q000737:chemistry / Q000201:enzymology*; D003545:Cysteine / Q000737:chemistry; D004229:Dithiothreitol / Q000737:chemistry; D004492:Edetic Acid / Q000737:chemistry; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000031:analogs & derivatives / Q000737:chemistry; D005033:Ethylmaleimide / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008623:Mercaptoethanol / Q000737:chemistry; D010618:Phenanthrolines / Q000737:chemistry; D012210:Rhamnose / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013379:Substrate Specificity; D020245:p-Chloromercuribenzoic Acid / Q000737:chemistry
chemical_list: D002414:Cations, Monovalent; D005656:Fungal Proteins; D010618:Phenanthrolines; D012834:Silver; D020245:p-Chloromercuribenzoic Acid; D008623:Mercaptoethanol; C041829:1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide methiodide; D004492:Edetic Acid; D006026:Glycoside Hydrolases; C022525:alpha-L-rhamnosidase; D003545:Cysteine; D005033:Ethylmaleimide; D012210:Rhamnose; D005022:Ethyldimethylaminopropyl Carbodiimide; D004229:Dithiothreitol; C025205:1,10-phenanthroline