title: In vitro effects of sporobacterin probiotic on the function of donor granulocyte-macrophage cells.
abstract: The effects of sporobacterin probiotic (Bakoren Company) on oxidative activity of donor granulocyte-macrophage cells (GMC) were studied in vitro by luminol-dependent chemiluminescent method, and the effects of the probiotic on the production of pro- and anti-inflammatory cytokines were evaluated by ELISA. The probiotic dose-dependently stimulated spontaneous production of free radicals by GMC; combined treatment with immunomodulators likopid, polyoxydonium, and IFN-α2a produced a more potent effect. Sporobacterin stimulated the production of pro- and anti-inflammatory cytokines in cell cultures. These data confirmed the immunomodulatory effect of sporobacterin, an important component in the phagocytic system cells.
authors.affiliation: VI Shumakov Center of Transplantology and Artificial Organs, Moscow, Russia.
authors.forename: N I
authors.initials: NI
authors.lastname: Gabrielyan
authors.forename: V S
authors.initials: VS
authors.lastname: Suskova
authors.forename: S I
authors.initials: SI
authors.lastname: Suskov
authors.forename: N L
authors.initials: NL
authors.lastname: Vologodskaya
mesh_terms: D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives; D000328:Adult; D001428:Bacterial Vaccines / Q000494:pharmacology*; D016207:Cytokines / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D055014:Granulocyte-Macrophage Progenitor Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D009930:Organic Chemicals; D019936:Probiotics / Q000494:pharmacology*
chemical_list: D001428:Bacterial Vaccines; D016207:Cytokines; D005609:Free Radicals; D009930:Organic Chemicals; C089161:glikopin; C089921:polyoxydonium; C495704:sporobacterin; D000119:Acetylmuramyl-Alanyl-Isoglutamine