title: Vaccine-related mumps infections in Thailand and the identification of a novel mutation in the mumps fusion protein.
abstract: An outbreak of nine cases of mumps was reported from a total of 97 vaccinated nursing students at two medical colleges in Thailand in 2010, 16-26 days after administration of MMR vaccine containing the L-Zagreb mumps strain. Symptoms ranged in severity from fever and parotid swelling to orchitis. Clinical samples were obtained from seven patients and three were suitable for further study. Sequencing confirmed that the SH gene of the mumps virus in the unpassaged clinical specimens was identical to the L-Zagreb SH gene in the vaccine. Further analysis of the viral genome identified nucleotide position 5170 as a novel mutation which corresponds to an amino acid change in the fusion protein. This study provides another virologically confirmed example of mumps resulting from the L-Zagreb vaccine strain.
authors.affiliation: Division of Virology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK. sarah.gilliland@nibsc.hpa.org.uk
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Gilliland
authors.forename: Adrian
authors.initials: A
authors.lastname: Jenkins
authors.forename: Lauren
authors.initials: L
authors.lastname: Parker
authors.forename: Nongyao
authors.initials: N
authors.lastname: Somdach
authors.forename: Sirima
authors.initials: S
authors.lastname: Pattamadilok
authors.forename: Patcha
authors.initials: P
authors.lastname: Incomserb
authors.forename: Neil
authors.initials: N
authors.lastname: Berry
authors.forename: Silke
authors.initials: S
authors.lastname: Schepelmann
authors.forename: Philip
authors.initials: P
authors.lastname: Minor
mesh_terms: D000595:Amino Acid Sequence; D004196:Disease Outbreaks; D005260:Female; D005334:Fever / Q000139:chemically induced / Q000276:immunology; D006801:Humans; D008297:Male; D022542:Measles-Mumps-Rubella Vaccine / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology*; D008969:Molecular Sequence Data; D009107:Mumps / Q000453:epidemiology / Q000276:immunology* / Q000821:virology; D009109:Mumps virus / Q000235:genetics / Q000276:immunology*; D009154:Mutation; D009920:Orchitis / Q000139:chemically induced / Q000276:immunology; D010306:Parotid Gland / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013785:Thailand / Q000453:epidemiology; D014760:Viral Fusion Proteins / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics; D055815:Young Adult
chemical_list: D022542:Measles-Mumps-Rubella Vaccine; D012367:RNA, Viral; D014760:Viral Fusion Proteins; D014764:Viral Proteins; C067386:small hydrophobic protein, Mumps virus