title: Inducing local DNA damage by visible light to study chromatin repair.
abstract: Dynamics of DNA repair and recruitment of repair factors to damaged DNA can be studied by live cell microscopy. DNA damage is usually inflicted by a laser beam illuminating a DNA-interacting photosensitizer in a small area of the nucleus. We demonstrate that a focused beam of visible low intensity light alone can inflict local DNA damage and permit studies of DNA repair, thus avoiding potential artifacts caused by exogenous photosensitizers.
authors.affiliation: Division of Cell Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 7, Gronostajowa St., 30-387 Kraków, Poland.
authors.forename: Kamil J
authors.initials: KJ
authors.lastname: Solarczyk
authors.forename: Mirosław
authors.initials: M
authors.lastname: Zarębski
authors.forename: Jurek W
authors.initials: JW
authors.lastname: Dobrucki
mesh_terms: D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D002843:Chromatin / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D003116:Color; D004249:DNA Damage; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004996:Ethidium / Q000009:adverse effects; D005455:Fluorescent Antibody Technique; D015894:Genome, Human / Q000528:radiation effects; D006367:HeLa Cells; D006657:Histones / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007834:Lasers / Q000009:adverse effects; D008027:Light; D018384:Oxidative Stress; D010766:Phosphorylation; D017319:Photosensitizing Agents / Q000009:adverse effects; D013997:Time Factors; D051758:Transcription Factor TFIIH / Q000235:genetics / Q000378:metabolism; D000076105:X-ray Repair Cross Complementing Protein 1
chemical_list: D002843:Chromatin; D004268:DNA-Binding Proteins; C468783:H2AX protein, human; D006657:Histones; D017319:Photosensitizing Agents; D000076105:X-ray Repair Cross Complementing Protein 1; D051758:Transcription Factor TFIIH; D004996:Ethidium