title: Comparison of the gene expression profiles between smokers with and without lung cancer using RNA-Seq.
abstract: Lung cancer seriously threatens human health, so it is important to investigate gene expression changes in affected individuals in comparison with healthy people. Here we compared the gene expression profiles between smokers with and without lung cancer. We found that the majority of the expressed genes (threshold was set as 0.1 RPKM) were the same in the two samples, with a small portion of the remainder being unique to smokers with and without lung cancer. Expression distribution patterns showed that most of the genes in smokers with and without lung cancer are expressed at low or moderate levels. We also found that the expression levels of the genes in smokers with lung cancer were lower than in smokers without lung cancer in general. Then we detected 27 differentially expressed genes in smokers with versus without lung cancer, and these differentially expressed genes were found to be involved in diverse processes. Our study provided detail expression profiles and expression changes between smokers with and without lung cancer.
authors.affiliation: Department of Oncology, PLA general hospital of Chengdu Commond, Clinical Medical College of Third Medical Military University, Chengdu, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Cheng
authors.forename: You
authors.initials: Y
authors.lastname: Cheng
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Zhao
authors.forename: Hui
authors.initials: H
authors.lastname: Gao
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D016022:Case-Control Studies; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012907:Smoking / Q000009:adverse effects*; D059467:Transcriptome
chemical_list: D014408:Biomarkers, Tumor; D012313:RNA