title: Renalase-specific polyclonal antibody and its application in the detection of renalase's expression.
abstract: Renalase is generated mainly by the kidneys, and renalase's expression in chronic kidney disease patients is reduced due to renal dysfunction. In this study, human renalase recombinant protein with prokaryotic expression was used for immunization of male New Zealand rabbits, and polyclonal antibodies against human renalase were obtained. To prepare and verify renalase polyclonal antibody, renalase recombinant protein was used as antigen and male New Zealand rabbits were immunized to obtain anti-serum for identification. On the basis of renalase antibody, the expression of renalase in renal tubular epithelial cells and renal tissue was detected. Two anti-renalase polyclonal antibodies were obtained. Renalase was constitutively expressed in human renal tubular epithelial cells, with the maximum expression in proximal convoluted tubules in renal tissue, and a small amount of expression in the glomeruli. Anti-renalase polyclonal antibodies were successfully prepared, which lays a foundation for further studies.
authors.affiliation: Department of Nephrology, Shanghai Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, China. zyzwq1030@163.com
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhao
authors.forename: Tao
authors.initials: T
authors.lastname: Xing
authors.forename: Junhui
authors.initials: J
authors.lastname: Li
authors.forename: Niansong
authors.initials: N
authors.lastname: Wang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000917:Antibody Formation; D000918:Antibody Specificity; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology / Q000201:enzymology*; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007114:Immunization; D007678:Kidney Glomerulus / Q000166:cytology / Q000201:enzymology*; D007684:Kidney Tubules / Q000166:cytology / Q000201:enzymology*; D008297:Male; D008995:Monoamine Oxidase / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology; D011817:Rabbits; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000906:Antibodies; D011994:Recombinant Proteins; D008995:Monoamine Oxidase; C521557:renalase