title: Identification and genetic characterization of new bovine viral diarrhea virus genotype 2 strains in pigs isolated in China.
abstract: Classical swine fever (CSF)-like symptoms in pigs regarded as free from CSF has been reported previously. From sick pigs with CSF-like symptoms, and who had been inoculated with the hog cholera vaccine, samples were collected and subjected to RT-PCR using specific primers. Twelve bovine viral diarrhea virus 2 (BVDV-2) strains were screened and isolated. Homology comparison showed that the E2 genes of the twelve isolates were highly conserved. The genome of the one of the BVDV-2 isolates (named as SH-28) from the sick pigs, which showed a noncytopathic effect in MDBK cell cultures and strong reactivity with monoclonal antibody (MAb) Bz-53 raised against BVDV-2, was sequenced. The genome of SH-28 comprises 12,279 nucleotides and contains a large open reading frame beginning at nucleotide 386 and ending at nucleotide 12,073. Genomic comparison and phylogenetic analyzes showed that SH-28 fall into BVDV-2 subtype and was most similar to XJ-04 (nucleotide and amino acid homologies were 89.9-93.8 % and 91.1-96.9 %, respectively), but was genetically divergent from ZM-95 (pig BVDV-1).
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Tao
authors.forename: Yin
authors.initials: Y
authors.lastname: Wang
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Jian-ye
authors.initials: JY
authors.lastname: Wang
authors.forename: Guo-qiang
authors.initials: GQ
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D002681:China; D016000:Cluster Analysis; D003967:Diarrhea / Q000662:veterinary* / Q000821:virology; D030242:Diarrhea Virus 2, Bovine Viral / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016679:Genome, Viral; D005838:Genotype; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D018182:Pestivirus Infections / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000821:virology*
chemical_list: D012367:RNA, Viral