title: Fast analysis of superoxide dismutase (SOD) activity in barley leaves using visible and near infrared spectroscopy.
abstract: Visible and near infrared (Vis/NIR) spectroscopy was investigated for the fast analysis of superoxide dismutase (SOD) activity in barley (Hordeum vulgare L.) leaves. Seven different spectra preprocessing methods were compared. Four regression methods were used for comparison of prediction performance, including partial least squares (PLS), multiple linear regression (MLR), least squares-support vector machine (LS-SVM) and Gaussian process regress (GPR). Successive projections algorithm (SPA) and regression coefficients (RC) were applied to select effective wavelengths (EWs) to develop more parsimonious models. The results indicated that Savitzky-Golay smoothing (SG) and multiplicative scatter correction (MSC) should be selected as the optimum preprocessing methods. The best prediction performance was achieved by the LV-LS-SVM model on SG spectra, and the correlation coefficients (r) and root mean square error of prediction (RMSEP) were 0.9064 and 0.5336, respectively. The conclusion was that Vis/NIR spectroscopy combined with multivariate analysis could be successfully applied for the fast estimation of SOD activity in barley leaves.
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. zjukww@163.com
authors.forename: Wenwen
authors.initials: W
authors.lastname: Kong
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: He
authors.forename: Tian
authors.initials: T
authors.lastname: Tian
authors.forename: Weijun
authors.initials: W
authors.lastname: Zhou
mesh_terms: D001467:Hordeum / Q000201:enzymology*; D016018:Least-Squares Analysis; D018515:Plant Leaves / Q000737:chemistry / Q000201:enzymology*; D015203:Reproducibility of Results; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D013482:Superoxide Dismutase / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D060388:Support Vector Machine
chemical_list: D013482:Superoxide Dismutase
keywords: Gaussian process regression; barley; least squares-support vector machine; superoxide dismutase; variable selection; visible and near infrared spectroscopy