title: Trypanosoma brucei FKBP12 differentially controls motility and cytokinesis in procyclic and bloodstream forms.
abstract: FKBP12 proteins are able to inhibit TOR kinases or calcineurin phosphatases upon binding of rapamycin or FK506 drugs, respectively. The Trypanosoma brucei FKBP12 homologue (TbFKBP12) was found to be a cytoskeleton-associated protein with specific localization in the flagellar pocket area of the bloodstream form. In the insect procyclic form, RNA interference-mediated knockdown of TbFKBP12 affected motility. In bloodstream cells, depletion of TbFKBP12 affected cytokinesis and cytoskeleton architecture. These last effects were associated with the presence of internal translucent cavities limited by an inside-out configuration of the normal cell surface, with a luminal variant surface glycoprotein coat lined up by microtubules. These cavities, which recreated the streamlined shape of the normal trypanosome cytoskeleton, might represent unsuccessful attempts for cell abscission. We propose that TbFKBP12 differentially affects stage-specific processes through association with the cytoskeleton.
authors.affiliation: Laboratory of Molecular Parasitology, IBMM, Université Libre de Bruxelles, Gosselies, Belgium.
authors.forename: Anaïs
authors.initials: A
authors.lastname: Brasseur
authors.forename: Brice
authors.initials: B
authors.lastname: Rotureau
authors.forename: Marjorie
authors.initials: M
authors.lastname: Vermeersch
authors.forename: Thierry
authors.initials: T
authors.lastname: Blisnick
authors.forename: Didier
authors.initials: D
authors.lastname: Salmon
authors.forename: Philippe
authors.initials: P
authors.lastname: Bastin
authors.forename: Etienne
authors.initials: E
authors.lastname: Pays
authors.forename: Luc
authors.initials: L
authors.lastname: Vanhamme
authors.forename: David
authors.initials: D
authors.lastname: Pérez-Morga
mesh_terms: D002465:Cell Movement; D048749:Cytokinesis; D018105:DNA, Kinetoplast / Q000378:metabolism; D016054:DNA, Protozoan / Q000378:metabolism; D005407:Flagella / Q000378:metabolism / Q000648:ultrastructure; D055785:Gene Knockdown Techniques; D008870:Microtubules / Q000378:metabolism; D021381:Protein Transport; D015800:Protozoan Proteins / Q000235:genetics / Q000502:physiology*; D034741:RNA, Small Interfering / Q000235:genetics; D022061:Tacrolimus Binding Protein 1A / Q000235:genetics / Q000378:metabolism*; D014346:Trypanosoma brucei brucei / Q000201:enzymology* / Q000254:growth & development / Q000648:ultrastructure
chemical_list: D018105:DNA, Kinetoplast; D016054:DNA, Protozoan; D015800:Protozoan Proteins; D034741:RNA, Small Interfering; D022061:Tacrolimus Binding Protein 1A