title: In vitro investigation of efficient photodynamic therapy using a nonviral vector; hemagglutinating virus of Japan envelope.
abstract: Photodynamic therapy (PDT) is a photochemical modality approved for cancer treatment. PDT has demonstrated efficacy in early stage lung cancer and esophageal cancer. The accumulation of photosensitizers in cancer cells is necessary to enhance the therapeutic benefits of PDT; however, photosensitizers have low uptake efficiency. To overcome this limitation, a drug delivery system, such as the hemagglutinating virus of Japan envelope (HVJ-E) vector, is required. In this study, the combination of PDT and HVJ-E was investigated for enhancing the efficacy of PDT. The photosensitizers that were evaluated included 5-aminolaevulinic acid (5-ALA), protoporphyrin IX (PPIX), and HVJ-PPIX. The uptake of the photosensitizers as increased twenty-fold with the addition of HVJ-E. The cytotoxicity of conventional 5-ALA was enhanced by the addition of HVJ-E vector. In conclusion, HVJ-E vector improved the uptake of photosensitizers and the PDT effect.
authors.affiliation: Osaka University, Graduate School of Engineering, Osaka 565-0871, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Sakai
authors.forename: Naohiro
authors.initials: N
authors.lastname: Fujimoto
authors.forename: Katsunori
authors.initials: K
authors.lastname: Ishii
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakamura
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Kaneda
authors.forename: Kunio
authors.initials: K
authors.lastname: Awazu
mesh_terms: D000622:Aminolevulinic Acid / Q000008:administration & dosage / Q000493:pharmacokinetics; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000378:metabolism; D051379:Mice; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D011524:Protoporphyrins / Q000008:administration & dosage / Q000493:pharmacokinetics; D029082:Sendai virus / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D014759:Viral Envelope Proteins / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics
chemical_list: D000970:Antineoplastic Agents; D017319:Photosensitizing Agents; D011524:Protoporphyrins; D014759:Viral Envelope Proteins; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX