title: Dietary alpha-linolenic acid does not enhance accumulation of omega-3 long-chain polyunsaturated fatty acids in barramundi (Lates calcarifer).
abstract: This study examined the effects of substituting fish oil and fish meal with a blend of alpha-linolenic acid (ALA, 18:3 n-3) rich vegetable oils (14%, w/w) and defatted poultry meal (34%, w/w) in a formulated diet, on growth and tissue fatty acid profiles in barramundi fingerlings. Results indicated that on average, while the ALA levels of the barramundi liver and fillet increased with increasing dietary ALA, there was no corresponding increase in the levels of the omega-3 (n-3) long chain polyunsaturated fatty acid (LCPUFA). Compared to fish consuming a commercial feed, which contained fish meal and fish oil, fish on the ALA diets grew slower, had a lower feed intake and lower n-3 LCPUFA levels in the tissues. Hepatic mRNA expression of Δ6 desaturase (FADS2) and elongase (ELOVL5/2) was ~10 fold and ~3 fold higher, respectively, in all the ALA dietary groups, relative to those fed the commercial feed. However, the level of expression of the two genes was not different between fish fed differing ALA levels. These data demonstrate that increasing the ALA level of the diet is not an appropriate strategy for replacing marine sources of n-3 LCPUFA in barramundi. It was also noted, however, that within the different ALA dietary groups there was a large amount of variation between individual fish in their tissue DHA levels, suggesting a significant heterogeneity in their capacity for conversion of ALA and/or retention of n-3 LCPUFA. When dietary ALA intakes were greater than 0.8% en, tissue DHA levels were inversely related to ALA intake, suggesting that high intake of dietary ALA may inhibit DHA synthesis.
authors.affiliation: FOODplus Research Centre, School of Agriculture, Food and Wine, University of Adelaide, South Australia, Australia.
authors.forename: Wei-Chun
authors.initials: WC
authors.lastname: Tu
authors.forename: Beverly S
authors.initials: BS
authors.lastname: Mühlhäusler
authors.forename: Michael J
authors.initials: MJ
authors.lastname: James
authors.forename: David A J
authors.initials: DA
authors.lastname: Stone
authors.forename: Robert A
authors.initials: RA
authors.lastname: Gibson
mesh_terms: D000123:Acetyltransferases / Q000235:genetics; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D004281:Docosahexaenoic Acids / Q000737:chemistry / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D050602:Linoleoyl-CoA Desaturase / Q000235:genetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D010473:Perciformes / Q000254:growth & development / Q000378:metabolism*; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017962:alpha-Linolenic Acid / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D015525:Fatty Acids, Omega-3; D010743:Phospholipids; D012333:RNA, Messenger; D017962:alpha-Linolenic Acid; D004281:Docosahexaenoic Acids; D050602:Linoleoyl-CoA Desaturase; D000123:Acetyltransferases