title: Microsatellite primers for an Amazonian lowland tropical tree, Protium subserratum (Burseraceae).
abstract: OBJECTIVE
  The first microsatellite primers were developed for Protium subserratum, a widespread Amazonian tree, to investigate genetic differentiation between populations found on clay, brown-sand, and white-sand soils. •
  
  
  RESULTS
  Seventeen primer pairs were identified from two individuals of P. subserratum found on white-sand and brown-sand soil types. Polymorphism was analyzed in 63 individuals from a total of three populations, each found on a different soil type. The primers amplified tetra-, tri-, and dinucleotide repeats with three to 24 alleles per locus. Excluding monomorphic loci, observed and expected heterozygosities ranged from 0 to 0.852 and 0.036 to 0.901, respectively. •
  
  
  CONCLUSIONS
  These new microsatellite markers will be useful in studies of genetic diversity, population differentiation, and gene flow across habitat types in P. subserratum.
authors.affiliation: Department of Integrative Biology, University of California, Berkeley, 1005 Valley Life Sciences Building #3140, Berkeley, California 94720 USA. tmisiewicz@berkeley.edu
authors.forename: Tracy M
authors.initials: TM
authors.lastname: Misiewicz
authors.forename: Carlos E A
authors.initials: CE
authors.lastname: Barbosa
authors.forename: Paul V A
authors.initials: PV
authors.lastname: Fine
mesh_terms: D029403:Burseraceae / Q000235:genetics* / Q000254:growth & development; D017931:DNA Primers / Q000235:genetics*; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D017753:Ecosystem; D014644:Genetic Variation; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D012987:Soil / Q000032:analysis; D014197:Trees / Q000235:genetics* / Q000254:growth & development; D014329:Tropical Climate
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D012987:Soil