title: Novel splenic antigen-presenting cells derive from a Lin(-) c-kit(lo) progenitor.
abstract: The main DC subsets in murine spleen arise from BM-derived precursors. Recently, a novel APC type was described in spleen. To determine if spleen contains the progenitors of this subset, a stromal coculture system was used to assess candidate progenitors for their hematopoietic potential. Here, the progenitor of that subset is identified as a spleen endogenous Lin(-)c-kit(lo) hematopoietic progenitor and is most highly enriched among the Lin(-)c-kit(lo)CD34(+) subset. Dendritic-like cells produced in vitro functionally resemble the previously described in vivo equivalent subset with high endocytic capacity and capability for antigen-specific activation of CD8(+) T cells but not CD4(+) T cells.
authors.affiliation: Australian National University, Canberra, ACT 0200, Australia.
authors.forename: Pravin
authors.initials: P
authors.lastname: Periasamy
authors.forename: Jonathan K H
authors.initials: JK
authors.lastname: Tan
authors.forename: Helen C
authors.initials: HC
authors.lastname: O'Neill
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D002469:Cell Separation; D018920:Coculture Techniques / Q000379:methods; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*; D005434:Flow Cytometry; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology* / Q000276:immunology; D013234:Stem Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*; D017154:Stromal Cells / Q000166:cytology