title: Recombinant snake venom prothrombin activators.
abstract: Three prothrombin activators; ecarin, which was originally isolated from the venom of the saw-scaled viper Echis carinatus, trocarin from the rough-scaled snake Tropidechis carinatus, and oscutarin from the Taipan snake Oxyuranus scutellatus, were expressed in mammalian cells with the purpose to obtain recombinant prothrombin activators that could be used to convert prothrombin to thrombin. We have previously reported that recombinant ecarin can efficiently generate thrombin without the need for additional cofactors, but does not discriminate non-carboxylated prothrombin from biologically active γ-carboxylated prothrombin. Here we report that recombinant trocarin and oscutarin could not efficiently generate thrombin without additional protein co-factors. We confirm that both trocarin and oscutarin are similar to human coagulation Factor X (FX), explaining the need for additional cofactors. Sequencing of a genomic fragment containing 7 out of the 8 exons coding for oscutarin further confirmed the similarity to human FX.
authors.affiliation: AstraZeneca R&D Bioscience, Mölndal, Sweden. ann.lovgren@astrazeneca.com
authors.forename: Ann
authors.initials: A
authors.lastname: Lövgren
mesh_terms: D000818:Animals; D010450:Endopeptidases / Q000235:genetics*; D005343:Fibrinolytic Agents / Q000378:metabolism*; D006801:Humans; D014757:Viper Venoms / Q000235:genetics*; D017819:Viperidae / Q000378:metabolism*
chemical_list: D005343:Fibrinolytic Agents; D014757:Viper Venoms; D010450:Endopeptidases
keywords: FX; coagulation Factor X; prothrombin activator; recombinant ecarin; recombinant oscutarin; recombinant trocarin; thrombin