title: [Analysis of the mRNA expression of the S100β protein in adipocytes of patients with diabetes mellitus, type 2].
abstract: OBJECTIVE
  This study aims to explore the possible relationship between the expression level of S100β protein mRNA with diabetes mellitus type 2 in adipocytes from patients with this disease in comparison with normoglycemic individuals.
  
  
  METHODS
  Samples of adipose tissue of eight patients from the coronary section of the Institute Dante Pazzanese of Cardiology (IDPC), four in Group Diabetes and four of Normoglycemic group, were evaluated by RT-PCR real time.
  
  
  RESULTS
  An increase around 15 times values, between the threshold cycle (ΔCt), of mRNA expression of S100β protein in adipocytes of the diabetes group was observed in comparison to the control group (p = 0.015).
  
  
  CONCLUSIONS
  Our results indicate, for the first time, that there is coexistence of increased expression of the S100β and the type 2 diabetes mellitus gene.
authors.affiliation: Laboratório de Neurociências, Departamento de Anatomia, Instituto de Ciências Biomédicas III, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: Mike Yoshio
authors.initials: MY
authors.lastname: Hamasaki
authors.forename: Mario Hiroyuki
authors.initials: MH
authors.lastname: Hirata
authors.forename: Rosario Dominguez Crespo
authors.initials: RD
authors.lastname: Hirata
authors.forename: Silvia Tchernin
authors.initials: ST
authors.lastname: Himelfarb
authors.forename: Leila Maria Guissoni
authors.initials: LM
authors.lastname: Campos
authors.forename: Maria Inês
authors.initials: MI
authors.lastname: Nogueira
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D009414:Nerve Growth Factors; D012333:RNA, Messenger; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins