title: A galvanotaxis assay for analysis of neural precursor cell migration kinetics in an externally applied direct current electric field.
abstract: The discovery of neural stem and progenitor cells (collectively termed neural precursor cells) (NPCs) in the adult mammalian brain has led to a body of research aimed at utilizing the multipotent and proliferative properties of these cells for the development of neuroregenerative strategies. A critical step for the success of such strategies is the mobilization of NPCs toward a lesion site following exogenous transplantation or to enhance the response of the endogenous precursors that are found in the periventricular region of the CNS. Accordingly, it is essential to understand the mechanisms that promote, guide, and enhance NPC migration. Our work focuses on the utilization of direct current electric fields (dcEFs) to promote and direct NPC migration - a phenomenon known as galvanotaxis. Endogenous physiological electric fields function as critical cues for cell migration during normal development and wound repair. Pharmacological disruption of the trans-neural tube potential in axolotl embryos causes severe developmental malformations(1). In the context of wound healing, the rate of repair of wounded cornea is directly correlated with the magnitude of the epithelial wound potential that arises after injury, as shown by pharmacological enhancement or disruption of this dcEF(2-3). We have demonstrated that adult subependymal NPCs undergo rapid and directed cathodal migration in vitro when exposed to an externally applied dcEF. In this protocol we describe our lab's techniques for creating a simple and effective galvanotaxis assay for high-resolution, long-term observation of directed cell body translocation (migration) on a single-cell level. This assay would be suitable for investigating the mechanisms that regulate dcEF transduction into cellular motility through the use of transgenic or knockout mice, short interfering RNA, or specific receptor agonists/antagonists.
authors.affiliation: Institute for Biomaterials and Biomedical Engineering, University of Toronto, Ontario, Canada.
authors.forename: Robart
authors.initials: R
authors.lastname: Babona-Pilipos
authors.forename: Milos R
authors.initials: MR
authors.lastname: Popovic
authors.forename: Cindi M
authors.initials: CM
authors.lastname: Morshead
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D003584:Cytological Techniques / Q000295:instrumentation / Q000379:methods*; D004558:Electric Stimulation; D004560:Electricity; D055724:Electrophysiological Phenomena; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology*