title: The target of daptomycin is absent from Escherichia coli and other gram-negative pathogens.
abstract: Antistaphylococcal agents commonly lack activity against Gram-negative bacteria like Escherichia coli owing to the permeability barrier presented by the outer membrane and/or the action of efflux transporters. When these intrinsic resistance mechanisms are artificially compromised, such agents almost invariably demonstrate antibacterial activity against Gram negatives. Here we show that this is not the case for the antibiotic daptomycin, whose target appears to be absent from E. coli and other Gram-negative pathogens.
authors.affiliation: Antimicrobial Research Centre and School of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom.
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Randall
authors.forename: Katherine R
authors.initials: KR
authors.lastname: Mariner
authors.forename: Ian
authors.initials: I
authors.lastname: Chopra
authors.forename: Alex J
authors.initials: AJ
authors.lastname: O'Neill
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects; D017576:Daptomycin / Q000494:pharmacology*; D016972:Enterobacter cloacae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D001936:Moraxella catarrhalis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D012486:Salmonella typhimurium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D017576:Daptomycin