title: Street rabies virus causes dendritic injury and F-actin depolymerization in the hippocampus.
abstract: Rabies is an acute viral infection of the central nervous system and is typically fatal in humans and animals; however, its pathogenesis remains poorly understood. In this study, the morphological changes of dendrites and dendritic spines in the CA1 region of the hippocampus were investigated in mice that were infected intracerebrally with an MRV strain of the street rabies virus. Haematoxylin and eosin and fluorescence staining analysis of brain sections from the infected mice showed very few morphological changes in the neuronal bodies and neuronal processes. However, we found a significant decrease in the number of dendritic spines. Primary neuronal cultures derived from the hippocampus of mice (embryonic day 16.5) that were infected with the virus also showed an obvious decrease in the number of dendritic spines. Furthermore, the decrease in the number of dendritic spines was related to the depolymerization of actin filaments (F-actin). We propose that the observed structural changes can partially explain the severe clinical disease that was found in experimental models of street rabies virus infections.
authors.affiliation: Nursing College, Beihua University, 3999 Huashan Road, Jilin 132013, PR China.|Key Laboratory of Zoonoses, Ministry of Education, Institute of Zoonoses, Jilin University, 5333 Xian Road, Changchun 130062, PR China.|Department of Pathophysiology, Norman Bethune College of Medicine, Jilin University, Xinming Road, Changchun 130021, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Song
authors.affiliation: Key Laboratory of Zoonoses, Ministry of Education, Institute of Zoonoses, Jilin University, 5333 Xian Road, Changchun 130062, PR China.|Department of Pathophysiology, Norman Bethune College of Medicine, Jilin University, Xinming Road, Changchun 130021, PR China.
authors.forename: Jinli
authors.initials: J
authors.lastname: Hou
authors.affiliation: Key Laboratory of Zoonoses, Ministry of Education, Institute of Zoonoses, Jilin University, 5333 Xian Road, Changchun 130062, PR China.|Department of Pathophysiology, Norman Bethune College of Medicine, Jilin University, Xinming Road, Changchun 130021, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Qiao
authors.affiliation: Department of Histology and Embryology, Norman Bethune College of Medicine, Jilin University, Xinming Road, Changchun, 130021, PR China.
authors.forename: Yanchao
authors.initials: Y
authors.lastname: Li
authors.affiliation: Medical Research Laboratory, Jilin Medical College, Jilin Road, Jilin 132013, PR China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Key Laboratory of Zoonoses, Ministry of Education, Institute of Zoonoses, Jilin University, 5333 Xian Road, Changchun 130062, PR China.|Department of Pathophysiology, Norman Bethune College of Medicine, Jilin University, Xinming Road, Changchun 130021, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Duan
authors.affiliation: Key Laboratory of Zoonoses, Ministry of Education, Institute of Zoonoses, Jilin University, 5333 Xian Road, Changchun 130062, PR China.|Department of Pathophysiology, Norman Bethune College of Medicine, Jilin University, Xinming Road, Changchun 130021, PR China.
authors.forename: Zhenhong
authors.initials: Z
authors.lastname: Guan
authors.affiliation: Key Laboratory of Zoonoses, Ministry of Education, Institute of Zoonoses, Jilin University, 5333 Xian Road, Changchun 130062, PR China.|Department of Pathophysiology, Norman Bethune College of Medicine, Jilin University, Xinming Road, Changchun 130021, PR China.
authors.forename: Maolin
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Zoonoses, Ministry of Education, Institute of Zoonoses, Jilin University, 5333 Xian Road, Changchun 130062, PR China.|Department of Pathophysiology, Norman Bethune College of Medicine, Jilin University, Xinming Road, Changchun 130021, PR China.
authors.forename: Liankun
authors.initials: L
authors.lastname: Sun
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D003712:Dendrites / Q000821:virology; D005455:Fluorescent Antibody Technique; D006624:Hippocampus / Q000473:pathology* / Q000821:virology; D006651:Histocytochemistry; D007150:Immunohistochemistry; D051379:Mice; D008813:Mice, Inbred ICR; D008853:Microscopy; D009474:Neurons / Q000821:virology*; D011820:Rabies virus / Q000472:pathogenicity*
chemical_list: D000199:Actins