title: Molecular-based identification of yeasts isolated from bovine clinical mastitis in Japan.
abstract: This study analyzed molecular-based identification of yeasts that associated with bovine clinical mastitis in Japan. Over 3,200 quarter milk samples from Holstein dairy cows collected in 2011 on Hokkaido and Honshu islands were examined. Yeast isolates were characterized by polymerase chain reaction amplification and sequencing of the D1/D2 region of the 26S rDNA. Molecular characterization confirmed that Candida spp. and Pichia spp. were most frequently isolated species. Our molecular analysis of mastitic milk samples demonstrated the prevalence of Pichia kudriavzevii(22/58) and Candida tropicalis(14/58). In addition, we demonstrated that molecular analysis of the D1/D2 region of the 26S rDNA is a rapid and reliable method for identifying clinically significant yeasts in dairy hygiene, including potentially new or emerging pathogenic species.
authors.affiliation: National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Tomohito
authors.initials: T
authors.lastname: Hayashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Sugita
authors.forename: Eiji
authors.initials: E
authors.lastname: Hata
authors.forename: Ken
authors.initials: K
authors.lastname: Katsuda
authors.forename: Enshi
authors.initials: E
authors.lastname: Zhang
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kiku
authors.forename: Kazue
authors.initials: K
authors.lastname: Sugawara
authors.forename: Tomomi
authors.initials: T
authors.lastname: Ozawa
authors.forename: Tomoko
authors.initials: T
authors.lastname: Matsubara
authors.forename: Takaaki
authors.initials: T
authors.lastname: Ando
authors.forename: Tetsu
authors.initials: T
authors.lastname: Obayashi
authors.forename: Takaaki
authors.initials: T
authors.lastname: Ito
authors.forename: Takahiro
authors.initials: T
authors.lastname: Yabusaki
authors.forename: Katsunori
authors.initials: K
authors.lastname: Kudo
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Masateru
authors.initials: M
authors.lastname: Koiwa
authors.forename: Toshio
authors.initials: T
authors.lastname: Oshida
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Tagawa
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kawai
mesh_terms: D000818:Animals; D002175:Candida / Q000302:isolation & purification*; D002417:Cattle; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005260:Female; D007564:Japan / Q000453:epidemiology; D008414:Mastitis, Bovine / Q000453:epidemiology / Q000382:microbiology*; D009181:Mycoses / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D010802:Phylogeny; D010843:Pichia / Q000302:isolation & purification*; D012335:RNA, Ribosomal / Q000235:genetics
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S