title: An extremely alkaline novel xylanase from a newly isolated Streptomyces strain cultivated in corncob medium.
abstract: Streptomyces sp. CS802, recently isolated from Korean soil, produced xylanase in corncob medium. An extracellular xylanase (Xyn802) was purified by a single-step gel filtration and biochemical properties were studied. It showed high activity in extremely alkaline condition with optimum pH at 12.0 and exhibited stability between pH 7.5 and 13.0. It produced xylobiose and xylotriose as the major products from xylan, suggesting its endoxylanase nature. N-terminal amino acid sequences of Xyn802 were ADRNANRD which are significantly different from the reported xylanase. The activity was enhanced by various detergents and a reducing agent and stable in various organic solvents. Xyn802 produced by utilizing corncob, an agro-waste material, might be a novel xylanase based on its peculiar biochemical characteristics, and it can be a suitable candidate for the production of xylooligosaccharides including other useful products.
authors.affiliation: Department of Pharmacy, Chosun University, Gwangju 501-759, South Korea.
authors.forename: Jaya Ram
authors.initials: JR
authors.lastname: Simkhada
authors.forename: Hah-Young
authors.initials: HY
authors.lastname: Yoo
authors.forename: Yun Hee
authors.initials: YH
authors.lastname: Choi
authors.forename: Seung Wook
authors.initials: SW
authors.lastname: Kim
authors.forename: Jin Cheol
authors.initials: JC
authors.lastname: Yoo
mesh_terms: D000595:Amino Acid Sequence; D002614:Chelating Agents / Q000494:pharmacology; D002951:Citrates / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry*; D046508:Culture Techniques / Q000379:methods*; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry / Q000378:metabolism*; D004795:Enzyme Stability; D005965:Glucuronates / Q000096:biosynthesis; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D007700:Kinetics; D008670:Metals / Q000494:pharmacology; D009844:Oligosaccharides / Q000096:biosynthesis; D000077559:Sodium Citrate; D013302:Streptomyces / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification*; D013379:Substrate Specificity; D013696:Temperature; D003313:Zea mays / Q000737:chemistry*
chemical_list: D002614:Chelating Agents; D002951:Citrates; D003470:Culture Media; D005965:Glucuronates; D007220:Industrial Waste; D008670:Metals; D009844:Oligosaccharides; C570991:xylooligosaccharide; D000077559:Sodium Citrate; D043364:Endo-1,4-beta Xylanases