title: Molecularly imprinted composite cryogel for albumin depletion from human serum.
abstract: A new composite protein-imprinted macroporous cryogel was prepared for depletion of albumin from human serum prior to use in proteom applications. Polyhydroxyethyl-methacylate-based molecularly imprinted polymer (MIP) composite cryogel was prepared with high gel fraction yields up to 83%, and its morphology and porosity were characterized by Fourier transform infrared, scanning electron microscopy, swelling studies, flow dynamics, and surface area measurements. Selective binding experiments were performed in the presence of competitive proteins human transferrin (HTR) and myoglobin (MYB). MIP composite cryogel exhibited a high binding capacity and selectivity for human serum albumin (HSA) in the presence of HTR and MYB. The competitive adsorption amount for HSA in MIP composite cryogel is 722.1 mg/dL in the presence of competitive proteins (HTR and MYB). MIP composite cryogel column was successfully applied in the fast protein liquid chromatography system for selective depletion of albumin in human serum. The depletion ratio was highly increased by embedding beads into cryogel (85%). Finally, MIP composite cryogel can be reused many times with no apparent decrease in HSA adsorption capacity.
authors.affiliation: Biochemistry Division, Department of Chemistry, Hacettepe University, Ankara, Turkey.
authors.forename: Müge
authors.initials: M
authors.lastname: Andaç
authors.forename: Gözde
authors.initials: G
authors.lastname: Baydemir
authors.forename: Handan
authors.initials: H
authors.lastname: Yavuz
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D000327:Adsorption; D000418:Albumins / Q000302:isolation & purification*; D000818:Animals; D001667:Binding, Competitive; D002851:Chromatography, High Pressure Liquid; D059985:Cryogels; D017743:Equipment Reuse; D006736:Horses; D006801:Humans; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D009211:Myoglobin / Q000737:chemistry*; D011102:Polyhydroxyethyl Methacrylate / Q000031:analogs & derivatives*; D016062:Porosity; D011485:Protein Binding; D044967:Serum / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014168:Transferrin / Q000737:chemistry*
chemical_list: D000418:Albumins; D059985:Cryogels; D009211:Myoglobin; D014168:Transferrin; D011102:Polyhydroxyethyl Methacrylate