title: Effect of different root canal irrigants on the sealing ability of two all-in-one self-etch adhesives: An in vitro study.
abstract: OBJECTIVE
  To evaluate the effect of different root canal irrigants on the sealing ability of two self-etch adhesives, using dye penetration method, within the pulp chamber.
  
  
  METHODS
  72 extracted human molars were divided into six groups, according to the irrigant and self-etch adhesives used. After de-roofing the pulp chamber with a carborundum disc, the pulp was extirpated using excavator and broaches. In 36 samples, pulp chambers were bonded with Xeno V after irrigation with either 2% CHX (Group1: Xe-CHX) ; 17% EDTA + 5.25% NaOCl (Group2: Xe- EDTA + NaOCl) or normal saline (Group3: Xe-NS) and in other half samples, pulp chambers were bonded with Adper Easy One after irrigation with either 2% CHX (Group 4: Ad-CHX); 17%EDTA + 5.25% NaOCl (Group5: Ad-EDTA+NaOCl) or normal saline (Group 6: Ad-NS). All the samples were restored with Filtek Z350. Ten teeth from each group were assessed for dye penetration. Two samples in each group were viewed under scanning electron microscope to observe resin-dentin interfacial adaptation. Data was statistically analyzed using Mann-Whitney and Kruskal Wallis tests at a significance level of P < 0.05.
  
  
  RESULTS
  MEAN MICROLEAKAGE SCORES AND STANDARD DEVIATION OBSERVED WERE: Group 1 (Xe-CHX): 2.8 ± 0.42, Group 2 (Xe-EDTA + NaOCl): 2.3 ± 0.82, Group 3 (Xe-NS): 2.9 ± 0.31, Group 4 (Ad-CHX): 1.2 ± 0.91, Group 5 (Ad-EDTA + NaOCl): 0.8 ± 0.63, Group 6 (Ad-NS): 1.9 ± 0.99.
  
  
  CONCLUSIONS
  Adper Easy One showed significantly less leakage than Xeno V when normal saline was used as an irrigant. EDTA treatment followed by NaOCl application significantly reduced microleakage and improved the interfacial adaptation with Adper Easy One. CHX did not significantly affect the sealing ability of both one-step self-etch adhesives tested.
authors.affiliation: Department of Conservative Dentistry and Endodontics, Kothiwal Dental College and Research Center, Moradabad, Uttar Pradesh, India.
authors.forename: Rashi
authors.initials: R
authors.lastname: Agrawal
authors.forename: Shashi Prabha
authors.initials: SP
authors.lastname: Tyagi
authors.forename: Rajni
authors.initials: R
authors.lastname: Nagpal
authors.forename: Chandrakar Chaman
authors.initials: CC
authors.lastname: Mishra
authors.forename: Udai Pratap
authors.initials: UP
authors.lastname: Singh
keywords: Dye penetration; pulp chamber dentin; root canal irrigants; sealing ability; self-etch adhesives