title: Biolistic transformation of Brugia malayi.
abstract: Biolistics has become a versatile tool for direct gene transfer to various cell and tissue types. Following its successful use on the parasitic nematode Ascaris suum, we developed and evaluated biolistics in the transfection of the model filarial parasite Brugia malayi. Biolistics was proven to be an efficient strategy for transfection of all life stages of the parasite and paved the way for studies on elements essential for promoter function and gene regulation of filarial parasites. Here we present a biolistics protocol for the transfection of B. malayi based on the Biolistics PDS 1000/He system and gold microcarriers.
authors.affiliation: Department of Biological Science, Ohio University, Zanesville, OH, USA. Higazi@ohio.edu
authors.forename: Tarig B
authors.initials: TB
authors.lastname: Higazi
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Unnasch
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D019470:Biolistics / Q000379:methods*; D017178:Brugia malayi / Q000235:genetics*; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics; D005260:Female; D017930:Genes, Reporter / Q000235:genetics; D006046:Gold / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000235:genetics; D049409:Luciferases, Firefly / Q000235:genetics; D008297:Male; D008863:Microspheres; D010957:Plasmids / Q000235:genetics; D014162:Transfection; D014170:Transformation, Genetic
chemical_list: D049452:Green Fluorescent Proteins; D006046:Gold; D004247:DNA; D049409:Luciferases, Firefly