title: Analysis of the chemical composition, acute toxicity and skin sensitivity of essential oil from rhizomes of Ligusticum chuanxiong.
abstract: BACKGROUND
  Ligusticum chuanxiong Hort. (Umbelliferae) is a plant used as medicine and food in China. The essential oil (EO) extracted from its rhizomes possesses many pharmacological activities. However, there have been no scientific reports in the modern literature on the safety of EO.
  
  
  OBJECTIVE
  The objective of this study was to conduct a chemical composition analysis and evaluate acute toxicity and skin sensitivity of EO from rhizomes of Ligusticum chuanxiong.
  
  
  METHODS
  The chemical composition of hydrodistilled EO was analyzed by gas chromatography-mass spectrometry (GC-MS) and was evaluated in animals for acute toxicity, skin irritation and sensitization tests.
  
  
  RESULTS
  Dozens of compounds were detected and the major components of EO were ligustilide and butylidenephthalide with relative contents of 67.46 and 5.06%, respectively. The oral and intra-peritoneal lethal doses of 50% (LD(50)) in mice were 7.23 g/kg (approximately 14,606 times of clinical dose used) and 2.25 g/kg (approximately 5091 times of clinical dose used), respectively. The doses of 0.115 and 0.23 g/kg EO (approximately 232.5 and 465 times of the respective clinical doses used) revealed slight irritation effects on rabbit skin, but 1g/kg EO (approximately 2020 times of clinical dose used) had no observable effect on guinea pig skin in the skin sensitization test.
  
  
  CONCLUSIONS
  These experimental results indicate that short term application of EO is probably safe within the range of its clinical doses, but the dose should be controlled for external use due to its slight skin irritation.
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Ting
authors.initials: T
authors.lastname: Han
authors.forename: Cheng-Hao
authors.initials: CH
authors.lastname: Yu
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Jiang
authors.forename: Cheng
authors.initials: C
authors.lastname: Peng
authors.forename: Xia
authors.initials: X
authors.lastname: Ran
authors.forename: Lu-Ping
authors.initials: LP
authors.lastname: Qin
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000032:analysis / Q000633:toxicity; D000818:Animals; D005260:Female; D006168:Guinea Pigs; D007509:Irritants / Q000737:chemistry / Q000633:toxicity*; D030004:Ligusticum; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009822:Oils, Volatile / Q000737:chemistry / Q000633:toxicity*; D011817:Rabbits; D027343:Rhizome / Q000737:chemistry; D012867:Skin / Q000187:drug effects; D023382:Toxicity Tests, Acute
chemical_list: D007509:Irritants; D009822:Oils, Volatile; C027820:ligustilide; D015107:4-Butyrolactone