title: [Cordyceps sinensis enhances lymphocyte proliferation and CD markers expression in simulated microgravity environment].
abstract: This study was aimed to explore the effect of cordyceps sinensis enhancing lymphocyte proliferation and surface CD marker expression in simulated microgravity environment. The splenic lymphocytes were separated from mice and cultured in the rotary cell culture system simulated microgravity environment. The cells were treated with different concentration of cordyceps sinensis solution (0, 6.25, 12.5, 25 and 50 µg/ml) for 24, 48 and 72 h respectively, then the cells were harvested, and analyzed for cell proliferation and the expression of cell surface markers (CD4 and CD8). The results showed that under simulated microgravity environment, the lymphocyte proliferation was inhibited. When the concentration of cordyceps sinensis was 25 or 50 µg/ml, the lymphocyte proliferation, CD4 and CD8 expressions all increased, but 50 µg/ml cordyceps sinensis could inhibit the proliferation ability with the time prolonging. It is concluded that the suitable concentration of cordyceps sinensis displayed the ability to enhance the lymphocyte proliferation and CD marker expression in simulated microgravity environment. These results may be valuable for screening drugs which can be potentially against immunosuppression under simulated microgravity.
authors.affiliation: Department of Advanced Interdisciplinary Studies, Academy of Military Medical Sciences, Beijing, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Hao
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Li
authors.forename: Zhi-Yan
authors.initials: ZY
authors.lastname: Du
authors.forename: Cui-Mi
authors.initials: CM
authors.lastname: Duan
authors.forename: Yan-Meng
authors.initials: YM
authors.lastname: Wang
authors.forename: Chang-Yong
authors.initials: CY
authors.lastname: Wang
authors.forename: Jing-Ping
authors.initials: JP
authors.lastname: Song
authors.forename: Lin-Jie
authors.initials: LJ
authors.lastname: Wang
authors.forename: Ying-Hui
authors.initials: YH
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D015704:CD4 Antigens / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D032661:Cordyceps; D007108:Immune Tolerance; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D011134:Polysaccharides / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D018474:Weightlessness Simulation
chemical_list: D015704:CD4 Antigens; D016827:CD8 Antigens; D011134:Polysaccharides