title: Cytotoxicity of bacterial metabolic products, including listeriolysin O, on leukocyte targets.
abstract: Bacterial toxins can exhibit anticancer activities. Here we investigated the anticancer effects of the listeriolysin O toxin produced by Listeria monocytogenes. We found that supernatants of Listeria monocytogenes strains (wild type, 1189, and 1190) were cytotoxic to the Jurkat cell line and human peripheral blood mononuclear cells (PBMC) in a concentration-dependent manner. The supernatant of strain 1044, not producing listeriolysin O, was inactive. The supernatants of Listeria strains were also cytotoxic toward B cells of chronic leukemia patients, with no significant differences in activities between strains. We also tested supernatants of Bacillus subtilis strains BR1-90, BR1-S, and BR1-89 producing listeriolysin O. BR1-S and BR1-89 were cytotoxic to PBMC and to Jurkat cells, the latter being more sensitive to the supernatants. BR1-90 was not hemolytic or cytotoxic to PBMC, but was cytotoxic to Jurkat cells in the concentration range of 10-30%, suggesting that listeriolysin O is selectively effective against T cells. Overall, the response of human peripheral blood mononuclear and human leukemia cell lines to bacteria supernatants containing listeriolysin O depended on the bacteria strain, target cell type, and supernatant concentration.
authors.affiliation: Department of Applied Microbiology, University of Warsaw, 02-096 Warsaw, Poland.
authors.forename: R
authors.initials: R
authors.lastname: Stachowiak
authors.forename: M
authors.initials: M
authors.lastname: Lyzniak
authors.forename: B K
authors.initials: BK
authors.lastname: Budziszewska
authors.forename: K
authors.initials: K
authors.lastname: Roeske
authors.forename: J
authors.initials: J
authors.lastname: Bielecki
authors.forename: G
authors.initials: G
authors.lastname: Hoser
authors.forename: J
authors.initials: J
authors.lastname: Kawiak
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism*; D001427:Bacterial Toxins / Q000008:administration & dosage* / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003603:Cytotoxins / Q000008:administration & dosage* / Q000378:metabolism; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D006801:Humans; D019169:Jurkat Cells; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology*; D008089:Listeria monocytogenes / Q000378:metabolism*; D009374:Neoplasms, Experimental / Q000188:drug therapy*
chemical_list: D001427:Bacterial Toxins; D003603:Cytotoxins; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; C038705:hlyA protein, Listeria monocytogenes