title: Oral immunogenicity of porcine reproductive and respiratory syndrome virus antigen expressed in transgenic banana.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) is a persistent threat of economically significant influence to the swine industry worldwide. Recombinant DNA technology coupled with tissue culture technology is a viable alternative for the inexpensive production of heterologous proteins in planta. Embryogenic cells of banana cv. 'Pei chiao' (AAA) have been transformed with the ORF5 gene of PRRSV envelope glycoprotein (GP5) using Agrobacterium-mediated transformation and have been confirmed. Recombinant GP5 protein levels in the transgenic banana leaves were detected and ranged from 0.021%-0.037% of total soluble protein. Pigs were immunized with recombinant GP5 protein by orally feeding transgenic banana leaves for three consecutive doses at a 2-week interval and challenged with PRRSV at 7 weeks postinitial immunization. A vaccination-dependent gradational increase in the elicitation of serum and saliva anti-PRRSV IgG and IgA was observed. Furthermore, significantly lower viraemia and tissue viral load were recorded when compared with the pigs fed with untransformed banana leaves. The results suggest that transgenic banana leaves expressing recombinant GP5 protein can be an effective strategy for oral delivery of recombinant subunit vaccines in pigs and can open new avenues for the production of vaccines against PRRSV.
authors.affiliation: Department of Horticulture and Landscape Architecture, National Taiwan University, Taiwan, Republic of China.
authors.forename: Hui-Ting
authors.initials: HT
authors.lastname: Chan
authors.forename: Min-Yuan
authors.initials: MY
authors.lastname: Chia
authors.forename: Victor Fei
authors.initials: VF
authors.lastname: Pang
authors.forename: Chian-Ren
authors.initials: CR
authors.lastname: Jeng
authors.forename: Yi-Yin
authors.initials: YY
authors.lastname: Do
authors.forename: Pung-Ling
authors.initials: PL
authors.lastname: Huang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000956:Antigens, Viral / Q000096:biosynthesis* / Q000235:genetics; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D028521:Musa / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000378:metabolism; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D012463:Saliva / Q000737:chemistry / Q000276:immunology; D013552:Swine; D014170:Transformation, Genetic; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism; D019562:Viral Load / Q000662:veterinary; D014765:Viral Vaccines / Q000008:administration & dosage*; D014766:Viremia / Q000517:prevention & control
chemical_list: D057134:Antibodies, Neutralizing; D000956:Antigens, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C114789:glycoprotein 5, PRRSV