title: Single step detection of HIV-1 proviral DNA and housekeeping β-actin gene from dried blood spots by a monoplex polymerase chain reaction.
abstract: There is a growing need for developing a simple, rapid, reliable and cost effective method for detection of HIV-1 for early diagnosis of the infection especially in developing countries and in resource limited settings. A method for simultaneous detection of the HIV-1 p17 gene and the house keeping human β-actin gene from dried blood spots (DBS) by a monoplex polymerase chain reaction (PCR) is described. Genomic DNA was extracted from 40 HIV-1 positive and 40 HIV-1 negative DBS and used as templates to amplify both the HIV-1 p17 and β-actin genes simultaneously under the same cycling condition by a single round PCR. This method of detection of HIV-1 may provide a simple, rapid and cost effective alternative in resource limited settings; however, it would require testing a larger number of samples before widespread use.
authors.affiliation: Department of Molecular Virology, National AIDS Research Institute, Bhosari, Pune, India.
authors.forename: Ipsita
authors.initials: I
authors.lastname: Choudhary
authors.forename: Vaishali
authors.initials: V
authors.lastname: Chimanpure
authors.forename: Ajit
authors.initials: A
authors.lastname: Patil
authors.forename: Robin
authors.initials: R
authors.lastname: Mukhopadhyaya
authors.forename: Ramesh
authors.initials: R
authors.lastname: Paranjape
authors.forename: Jayanta
authors.initials: J
authors.lastname: Bhattacharya
mesh_terms: D000199:Actins / Q000097:blood* / Q000235:genetics; D004279:DNA, Viral / Q000097:blood* / Q000235:genetics; D059788:Dried Blood Spot Testing; D015488:HIV Antigens / Q000097:blood* / Q000235:genetics; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000097:blood* / Q000235:genetics
chemical_list: D000199:Actins; D004279:DNA, Viral; D015488:HIV Antigens; D054301:gag Gene Products, Human Immunodeficiency Virus; C054858:p17 protein, Human Immunodeficiency Virus Type 1