title: Mutant malonyl-CoA synthetases with altered specificity for polyketide synthase extender unit generation.
abstract: Tailoring guide: We have used structure-guided saturation mutagenesis followed by colorimetric screening to identify mutant malonyl-CoA synthetases with altered substrate specificity. One particular mutant displayed a 240-fold shift in specificity (see graphic). These mutant enzymes will be useful tools for providing extender units to probe the activity of polyketide synthases.
authors.affiliation: Department of Chemistry, North Carolina State University, Raleigh, NC 27695-8204, USA.
authors.forename: Irina
authors.initials: I
authors.lastname: Koryakina
authors.forename: Gavin J
authors.initials: GJ
authors.lastname: Williams
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D003066:Coenzyme A Ligases / Q000235:genetics* / Q000378:metabolism*; D016296:Mutagenesis; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D048630:Polyketide Synthases / Q000378:metabolism*; D012231:Rhizobium / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D050505:Mutant Proteins; D048630:Polyketide Synthases; D003066:Coenzyme A Ligases; C021533:malonyl-CoA synthetase