title: Giant vesicles as encapsulating matrix for stabilizing alcohol oxidase and as container for coupled enzymatic reactions.
abstract: Encapsulation of a multimeric alcohol oxidase (AOx) in giant vesicles (GVs) was prepared by rapid evaporation method. The specific activity (477 U/mg-protein) of the GV encapsulated AOx was 3.5 fold higher than the free AOx. The half-life (˜59.4 hours) of the encapsulated AOx was 20-fold higher than the free enzyme at 30°C. Further, the GV was used to encapsulate AOx and HRP together as coupled reaction catalyst for chromogen-based optical detection of alcohols. The results confirmed potential application of the GV as micro-dimensional container for enhancing stability, activity and biosensing applications of AOx.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India. sabitoj@alumni.iitg.ernet.in
authors.forename: Sabitoj S
authors.initials: SS
authors.lastname: Virk
authors.forename: Vishwa J
authors.initials: VJ
authors.lastname: Baruah
authors.forename: Pranab
authors.initials: P
authors.lastname: Goswami
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000378:metabolism*; D001230:Aspergillus / Q000201:enzymology; D002214:Capsules; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D008081:Liposomes / Q000737:chemistry*
chemical_list: D002214:Capsules; D004800:Enzymes, Immobilized; D008081:Liposomes; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase