title: Influence of hybrid inorganic/organic mesoporous and nanostructured materials on the cephalosporins' efficacy on different bacterial strains.
abstract: The aim of this study was to investigate the effect of different hybrid inorganic-organic micro- and nanomaterials (Fe(3)O(4)/PEG(600), Fe(3)O(4)/C(12), ZSM-5) on the antibacterial activity of different cephalosporins against Gram-positive and Gram-negative bacterial strains. The synergic effect of the studied materials was demonstrated by the increase in the growth inhibition zones diameter. All tested hybrid micro- and nanomaterials increased the activity of cefotaxime against Staphylococcus aureus. ZSM-5 increased the activity of cefotaxime and ceftriaxone and Fe(3)O(4)/C(12) that of ceftriaxone against Pseudomonas aeruginosa and S. aureus. The anti-Pseudomonas, anti-Klebsiella pneumoniae and anti-Bacillus subtilis activity of cefoperazone was increased by Fe(3)O(4)/C(12) nanoparticles, while the ZSM-5 improved its anti-Escherichia coli, K. pneumoniae, S. aureus and B. subtilis activity, whereas Fe(3)O(4)/PEG(600) against K. pneumoniae. The anti-K. pneumoniae activity of cefepime was increased by all tested nanoparticles, whereas its anti-B. subtilis and anti-E. coli activity was improved by Fe(3)O(4)/C(12) and Fe(3)O(4)/PEG(600) nanoparticles. In conclusion, both magnetic Fe(3)O(4) nanoparticles, charged outside as extra-shell with the antibiotic as well as ZSM-5 microparticles carrying the antibiotic inside the pores, significantly and specifically improved cephalosporin efficacy. A probable explanation for the increase in the antibiotic efficiency is the better penetration through the cellular wall of the antibiotic charged nanoparticles.
authors.affiliation: University of Bucharest, Faculty of Biology, Department of Microbiology and Immunology, Bucharest, Romania.
authors.forename: M
authors.initials: M
authors.lastname: Carmen Chifiriuc
authors.forename: D
authors.initials: D
authors.lastname: Mihaiescu
authors.forename: E
authors.initials: E
authors.lastname: Ilinca
authors.forename: L
authors.initials: L
authors.lastname: Marutescu
authors.forename: G
authors.initials: G
authors.lastname: Mihaescu
authors.forename: A
authors.initials: A
authors.lastname: Mihai Grumezescu
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D018407:Bacterial Physiological Phenomena / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002511:Cephalosporins / Q000737:chemistry* / Q000494:pharmacology*; D004058:Diffusion; D004337:Drug Carriers / Q000737:chemistry*; D007287:Inorganic Chemicals / Q000737:chemistry*; D053769:Nanocapsules / Q000737:chemistry*; D009930:Organic Chemicals / Q000737:chemistry*; D016062:Porosity; D013045:Species Specificity
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D004337:Drug Carriers; D007287:Inorganic Chemicals; D053769:Nanocapsules; D009930:Organic Chemicals