title: Cytotoxic constituents from the stem bark of Diospyros cuneata Standl.
abstract: A phytochemical investigation of the stem bark of Diospyros cuneata (Ebenaceae) together with in vitro cytotoxic evaluation of the pure compounds in four human cancer cell lines KB, Hep-2, HeLa and SiHa led to the isolation of plumbagin, elliptinone, lupeol, atraric acid methyl ester, maritinone, betulin and betulinaldehyde. The structural determination of the compounds was established by nuclear magnetic resonance, electron ionisation mass spectrometric analysis as well as comparison with data from the literature. Plumbagin exhibited a potent cytotoxic activity (CC50 = 3.56 µg mL(-1)) against KB cell lines whereas maritinone displayed cytotoxic activity against Hep-2 (CC50 = 17.30 µg mL(-1)), cervical cancer (CC50 = 21.10 µg mL(-1)) and the KB cell lines (CC50 = 20.30 µg mL(-1)). This is the first report on a phytochemical and biological evaluation of D. cuneata.
authors.affiliation: Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Colonia Chuburná de Hidalgo, Mérida, Yucatán 97200, México.
authors.forename: C
authors.initials: C
authors.lastname: Quintal-Novelo
authors.forename: R E
authors.initials: RE
authors.lastname: Moo-Puc
authors.forename: J
authors.initials: J
authors.lastname: Chale-Dzul
authors.forename: M
authors.initials: M
authors.lastname: Cáceres-Farfán
authors.forename: M
authors.initials: M
authors.lastname: Mendez-Gonzalez
authors.forename: R
authors.initials: R
authors.lastname: Borges-Argáez
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D029788:Diospyros / Q000737:chemistry*; D006801:Humans; D009285:Naphthoquinones / Q000737:chemistry; D053978:Pentacyclic Triterpenes / Q000737:chemistry; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018547:Plant Stems / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry; D000094062:Betulinic Acid; D000094042:Lupanes
chemical_list: D009285:Naphthoquinones; D053978:Pentacyclic Triterpenes; D010936:Plant Extracts; D014315:Triterpenes; C489087:maritinone; C002503:betulin; D000094062:Betulinic Acid; C010480:lupeol; D000094042:Lupanes; C014758:plumbagin