title: Aging and sequential resistance exercise bout effects on housekeeping gene messenger RNA expression in human skeletal muscle.
abstract: The purpose of this study was to investigate how age and 1 week of conventional resistance exercise affects commonly used housekeeping gene (HKG) messenger RNAs (mRNAs) in skeletal muscle. Ten college-aged (18-25 years) and 10 older (60-76 years) men completed 3 lower-body resistance exercise bouts on Monday, Wednesday, and Friday, and muscle samples were obtained before bout 1 (T1), 48 hours after the first (T2) and second bouts (T3), and 24 hours after the third bout (T4). Raw Ct values indicated that β-actin and cyclophilin were more highly expressed in older vs. younger males (p < 0.01) at T1. When normalizing each HKG mRNA to the other 4 HKG mRNAs, CYC increased at T3 and glyceraldehyde-3-phosphate dehydrogenase decreased at T2 (p < 0.05) in younger men. This is one of the few studies to suggest that explicit HKG mRNAs should be used depending upon age group and resistance exercise intervention.
authors.affiliation: Department of Health and Exercise Science, University of Oklahoma, Norman, OK, USA.
authors.forename: Kyle L
authors.initials: KL
authors.lastname: Sunderland
authors.forename: Michael D
authors.initials: MD
authors.lastname: Roberts
authors.forename: Vincent J
authors.initials: VJ
authors.lastname: Dalbo
authors.forename: Chad M
authors.initials: CM
authors.lastname: Kerksick
mesh_terms: D000199:Actins / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging / Q000235:genetics*; D000704:Analysis of Variance; D021983:Cyclophilins / Q000378:metabolism; D015870:Gene Expression; D020043:Genes, Essential / Q000235:genetics*; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D055070:Resistance Training
chemical_list: D000199:Actins; D012333:RNA, Messenger; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating); D021983:Cyclophilins