title: Detection of a UL97 gene mutation conferring ganciclovir resistance in human cytomegalovirus: prevalence of the D605E polymorphism in Korean immunocompromised patients.
abstract: The purpose of this study was to determine the prevalence of ganciclovir (GCV) resistance-conferring human cytomegalovirus (HCMV) UL97 gene mutations and UL97 polymorphisms in Korean immunocompromised patients. A partial sequence of the HCMV UL97 gene spanning codons 430 to 644 was amplified in 77 samples from 32 patients by nested polymerase chain reaction (PCR) and sequenced directly. A cysteine-to-glycine mutation at codon 592 (C592G) conferring GCV resistance was detected in a 2-year-old girl after a 40-day GCV treatment, but overall, UL97 gene mutations associated with GCV resistance were rare in GCV-treated patients. An aspartate-to-glutamate substitution at codon 605 (D605E) was detected in 29 of 32 patients (90.6%), but 17 of 19 (89.5%) GCV-naïve patients also possessed D605E. These findings indicate that the D605E polymorphism, which is frequent in Korean patients and thus may be a natural sequence variant, could be a genetic marker for HCMV in Asian countries.
authors.forename: Heungsup
authors.initials: H
authors.lastname: Sung
authors.forename: Dongheui
authors.initials: D
authors.lastname: An
authors.forename: Sang-Oh
authors.initials: SO
authors.lastname: Lee
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Choi
authors.forename: Sung-Han
authors.initials: SH
authors.lastname: Kim
authors.forename: Hyun-Sook
authors.initials: HS
authors.lastname: Chi
authors.forename: Mi-Na
authors.initials: MN
authors.lastname: Kim
mesh_terms: D001483:Base Sequence; D003586:Cytomegalovirus Infections / Q000453:epidemiology* / Q000821:virology; D017931:DNA Primers / Q000235:genetics; D024882:Drug Resistance, Viral / Q000235:genetics*; D015774:Ganciclovir; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D016867:Immunocompromised Host; D008969:Molecular Sequence Data; D020125:Mutation, Missense / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D056910:Republic of Korea / Q000453:epidemiology; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D005819:Genetic Markers; D017853:Phosphotransferases (Alcohol Group Acceptor); C075365:ganciclovir kinase; D015774:Ganciclovir