title: Tyrosine sulfation in a Gram-negative bacterium.
abstract: Tyrosine sulfation, a well-characterized post-translation modification in eukaryotes, has not previously been reported in prokaryotes. Here, we demonstrate that the RaxST protein from the Gram-negative bacterium, Xanthomonas oryzae pv. oryzae, is a tyrosine sulfotransferase. We used a newly developed sulfotransferase assay and ultraviolet photodissociation mass spectrometry to demonstrate that RaxST catalyses sulfation of tyrosine 22 of the Xoo Ax21 (activator of XA21-mediated immunity) protein. These results demonstrate a previously undescribed post-translational modification in a prokaryotic species with implications for studies of host immune responses and bacterial cell-cell communication systems.
authors.affiliation: Department of Plant Pathology and the Genome Center, University of California, One Shields Ave, Davis, California 95616, USA.
authors.forename: Sang-Wook
authors.initials: SW
authors.lastname: Han
authors.forename: Sang-Won
authors.initials: SW
authors.lastname: Lee
authors.forename: Ofir
authors.initials: O
authors.lastname: Bahar
authors.forename: Benjamin
authors.initials: B
authors.lastname: Schwessinger
authors.forename: Michelle R
authors.initials: MR
authors.lastname: Robinson
authors.forename: Jared B
authors.initials: JB
authors.lastname: Shaw
authors.forename: James A
authors.initials: JA
authors.lastname: Madsen
authors.forename: Jennifer S
authors.initials: JS
authors.lastname: Brodbelt
authors.forename: Pamela C
authors.initials: PC
authors.lastname: Ronald
mesh_terms: D057075:Enzyme Assays / Q000379:methods; D056951:Photoelectron Spectroscopy / Q000379:methods; D011499:Protein Processing, Post-Translational; D013379:Substrate Specificity; D015238:Sulfotransferases / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism*; D014974:Xanthomonas / Q000378:metabolism*
chemical_list: D014443:Tyrosine; D015238:Sulfotransferases