title: Wash-less and highly sensitive assay for prostate specific antigen detection.
abstract: A novel, simple and facile biosensor for prostate specific antigen (PSA) detection was constructed. In this method, proteolytically active PSA is capable of cleaving PSA substrate-magnetic carrier complexes. Electrochemical analysis of the sensor layer showed a significant decrease in impedance signal after proteolysis has occurred, whereas a positive change in impedance was observed using a negative control substrate, indicating the specificity of our detection mechanism. The biosensor has the ability to monitor PSA concentrations ranging from 1 pg ml(-1) to 1 μg ml(-1) with a detection limit as low as 1 pg ml(-1). The sensor offers the possibility of developing a wash-less and cost-effective point-of-care device due to the simplicity of the probe immobilization process and the elimination of labeling and reporter molecules during the biosensing step.
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmacy, The University of Jordan, Amman 11942, Jordan.
authors.forename: Ghadeer A R Y
authors.initials: GA
authors.lastname: Suaifan
authors.forename: Chiheb
authors.initials: C
authors.lastname: Esseghaier
authors.forename: Andy
authors.initials: A
authors.lastname: Ng
authors.forename: Mohammed
authors.initials: M
authors.lastname: Zourob
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D017097:Electric Impedance; D055664:Electrochemical Techniques / Q000379:methods; D006801:Humans; D057230:Limit of Detection; D008280:Magnetics; D008297:Male; D017430:Prostate-Specific Antigen / Q000032:analysis*
chemical_list: D017430:Prostate-Specific Antigen