title: Specific detection and localization of microsporidian parasites in invertebrate hosts by using in situ hybridization.
abstract: We designed fluorescence in situ hybridization probes for two distinct microsporidian clades and demonstrated their application in detecting, respectively, Nosema/Vairimorpha and Dictyoceola species. We used them to study the vertical transmission of two microsporidia infecting the amphipod Gammarus duebeni.
authors.affiliation: School of Biology, University of Leeds, Leeds, United Kingdom. aurore_dubuffet@yahoo.fr
authors.forename: Aurore
authors.initials: A
authors.lastname: Dubuffet
authors.forename: Judith E
authors.initials: JE
authors.lastname: Smith
authors.forename: Leellen
authors.initials: L
authors.lastname: Solter
authors.forename: M Alejandra
authors.initials: MA
authors.lastname: Perotti
authors.forename: Henk R
authors.initials: HR
authors.lastname: Braig
authors.forename: Alison M
authors.initials: AM
authors.lastname: Dunn
mesh_terms: D033304:Amphipoda / Q000382:microbiology*; D000818:Animals; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D016814:Microsporidia / Q000235:genetics / Q000302:isolation & purification*; D015345:Oligonucleotide Probes / Q000235:genetics
chemical_list: D015345:Oligonucleotide Probes