title: Radioprotective activity and cytogenetic effect of resveratrol in human lymphocytes: an in vitro evaluation.
abstract: Trans-resveratrol is a natural occurring polyphenol, obtained from grapes and other berries. This compound has shown antioxidant, anti-inflammatory, immunostimulant or anti-carcinogenic properties. Our aim was to evaluate the radioprotective efficacy, in vitro, of trans-resveratrol against radiation-induced chromosomal damage and to study the genotoxicity and cytotoxicity of this polyphenol in cell cultures without irradiation. The study was carried out by the pre-treatment of human lymphocytes at concentrations from 0 to 219μM of trans-resveratrol. The results showed that all concentrations tested reduced radiation-induced chromosomal damage compared with cells with any treatment. Maximum damage protection was observed at the concentration of 2.19μM. Concerning genotoxic results, all tested trans-resveratrol concentrations increased the sister chromatid exchange (SCE) index compared with no trans-resveratrol treatment. Cytotoxic indexes (Mitotic and Proliferation Index) showed that the lowest concentrations could enhance the cell proliferation rates and the highest ones could negatively affect to human peripheral lymphocytes growth.
authors.affiliation: Departament of Preventive Medicine and Public Health, Faculty of Pharmacy, University of Valencia, 46100 Burjassot, Spain.
authors.forename: Natividad
authors.initials: N
authors.lastname: Sebastià
authors.forename: Miguel
authors.initials: M
authors.lastname: Almonacid
authors.forename: Juan Ignacio
authors.initials: JI
authors.lastname: Villaescusa
authors.forename: José
authors.initials: J
authors.lastname: Cervera
authors.forename: Esperanza
authors.initials: E
authors.lastname: Such
authors.forename: María Angeles
authors.initials: MA
authors.lastname: Silla
authors.forename: Jose Miguel
authors.initials: JM
authors.lastname: Soriano
authors.forename: Alegría
authors.initials: A
authors.lastname: Montoro
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D004305:Dose-Response Relationship, Drug; D005720:Gamma Rays / Q000009:adverse effects; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000528:radiation effects*; D008940:Mitotic Index; D009152:Mutagenicity Tests; D011837:Radiation-Protective Agents / Q000009:adverse effects / Q000494:pharmacology*; D000077185:Resveratrol; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013267:Stilbenes / Q000494:pharmacology* / Q000633:toxicity*
chemical_list: D011837:Radiation-Protective Agents; D013267:Stilbenes; D000077185:Resveratrol