title: [Direct proteomic profiling of human urine and blood serum in an experiment with 5-day dry immersion].
abstract: Changes in proteome of urine and blood serum obtained from 14 healthy humans (age 21-29 yrs) medically certified for an experiment with dry immersion were analyzed. Urine and serum samples were pre-fractionated and enriched with magnetic particles MB-WCX and MB-HIC, respectively, on robot ClinProt (Bruker Daltonics) for direct mass-spectrometry profiling by MALDI-TOF. As a result, 143 protein peaks on the average were identified in urine samples. It was shown that a high variation coefficient in 23.7% of protein peaks, i.e. double technical, points to the most plastic fraction of the urine proteome. In blood serum, 175 peaks were identified in a sample on the average. Comparison of baseline and immersion mass-spectra of the blood proteome revealed significant differences. Increased peak areas of several protein fragments--C3 and C4 fragments of complement system, high-molecular kininogen and fibrinogen--can be ascribed to human body adaptation to the experimental conditions.
mesh_terms: D000222:Adaptation, Physiological; D000328:Adult; D003176:Complement C3 / Q000032:analysis; D003181:Complement C4 / Q000032:analysis; D005340:Fibrinogen / Q000032:analysis; D006801:Humans; D007101:Immersion; D007704:Kininogens / Q000032:analysis; D008280:Magnetics; D008297:Male; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D055815:Young Adult
chemical_list: D003176:Complement C3; D003181:Complement C4; D007704:Kininogens; D020543:Proteome; D005340:Fibrinogen