title: Comparison of immunologic and molecular assays for the diagnosis of gastrointestinal viral infections.
abstract: The performance of immunochromatographic and enzyme-linked immunosorbent assays (RIDA®QUICK and RIDASCREEN®, R-Biopharm) was compared with real-time reverse transcription-polymerase chain reaction techniques for the diagnosis of gastrointestinal viral infections. The sensitivity of the immunologic methods was comparable to polymerase chain reaction for the diagnosis of rotavirus and astrovirus, while it was reduced for detection of norovirus and adenovirus.
authors.affiliation: S.S. Virologia Molecolare, S.C. Virologia e Microbiologia, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Rovida
authors.forename: Giulia
authors.initials: G
authors.lastname: Campanini
authors.forename: Antonella
authors.initials: A
authors.lastname: Sarasini
authors.forename: Kodjo Messan Guy
authors.initials: KM
authors.lastname: Adzasehoun
authors.forename: Antonio
authors.initials: A
authors.lastname: Piralla
authors.forename: Fausto
authors.initials: F
authors.lastname: Baldanti
mesh_terms: D000256:Adenoviridae / Q000302:isolation & purification; D000328:Adult; D002675:Child, Preschool; D002846:Chromatography, Affinity / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005759:Gastroenteritis / Q000175:diagnosis* / Q000821:virology*; D006801:Humans; D007223:Infant; D001257:Mamastrovirus / Q000302:isolation & purification; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D029322:Norovirus / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012401:Rotavirus / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*; D014777:Virus Diseases / Q000175:diagnosis* / Q000821:virology*