title: Identification of microbiota present on the surface of Taleggio cheese using PCR-DGGE and RAPD-PCR.
abstract: Microbial DNA from 9 batches of Taleggio PDO cheese sampled at various times during ripening, brines, swabs of wooden shelves used for cheese dry-salting, and 13 commercial cheeses were analyzed by denaturing gradient gel electrophoresis (PCR-DGGE) and/or random amplification of polymorphic DNA (RAPD-PCR). Sequencing allowed the detection of 12 genera, 27 species, and 2 unclassified bacteria. Molecular analysis allowed for the detection of microorganisms not previously associated with Taleggio such as Lactobacillus paracasei, Carnobacterium maltaromaticum, Bacillus licheniformis, Corynebacterium variabile, Psychrobacter cibarius, and Staphylococcus carnosus. For the first time Massilia spp. was detected in a dairy ecosystem.
  
  
  CONCLUSIONS
  Indigenous species and strains of bacteria identified by this study could be used for the selection of dairy cultures to be employed routinely by manufacturers to control the Taleggio cheese production. The new cultures may give the bases for driving dairy processes and, consequently, control the typical flavor resulting from metabolic actions of environmental microorganisms.
authors.affiliation: Laboratorio Qualità dei Prodotti, Istituto Sperimentale Italiano Lazzaro Spallanzani, Via Einstein, Localitá Cascina Codazza, 26900 Lodi, Italy. maria.feligini@istitutospallanzani.it
authors.forename: Maria
authors.initials: M
authors.lastname: Feligini
authors.forename: Simona
authors.initials: S
authors.lastname: Panelli
authors.forename: Joanna Natalia
authors.initials: JN
authors.lastname: Buffoni
authors.forename: Cesare
authors.initials: C
authors.lastname: Bonacina
authors.forename: Christian
authors.initials: C
authors.lastname: Andrighetto
authors.forename: Angiolella
authors.initials: A
authors.lastname: Lombardi
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics; D002611:Cheese / Q000032:analysis* / Q000382:microbiology*; D016000:Cluster Analysis; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis / Q000379:methods; D017753:Ecosystem; D005516:Food Microbiology; D054892:Metagenome; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S