title: An acrylamide biosensor based on immobilization of hemoglobin onto multiwalled carbon nanotube/copper nanoparticles/polyaniline hybrid film.
abstract: A method is described for the construction of a highly sensitive electrochemical biosensor for the detection of acrylamide, based on covalent immobilization of hemoglobin (Hb) onto carboxylated multiwalled carbon nanotube/copper nanoparticle/polyaniline (c-MWCNT/CuNP/PANI) composite electrodeposited onto pencil graphite (PG) electrode. The enzyme electrode was characterized by cyclic voltammetry (CV), scanning electron microscopy (SEM), X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, and electrochemical impedance spectroscopy (EIS). The biosensor showed an optimal response at pH 5.5 (0.1 M sodium acetate buffer) and 35 °C when operated at 20 mV s(-1). The biosensor exhibited low detection limit (0.2 nM) with high sensitivity (72.5 μA/nM/cm(2)), fast response time (<2 s), and wide linear range (5 nM to 75 mM). Analytical recovery of added acrylamide was 95.40 to 97.56%. Within- and between-batch coefficients of variation were 2.35 and 4.50%, respectively. The enzyme electrode was used 120 times over a period of 100 days, when stored at 4 °C.
authors.affiliation: Department of Biochemistry, M.D. (Maharshi Dayanand) University, Rohtak 124001, India.
authors.forename: Bhawna
authors.initials: B
authors.lastname: Batra
authors.forename: Suman
authors.initials: S
authors.lastname: Lata
authors.forename: Madhu
authors.initials: M
authors.lastname: Sharma
authors.forename: C S
authors.initials: CS
authors.lastname: Pundir
mesh_terms: D020106:Acrylamide / Q000032:analysis*; D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D003300:Copper / Q000737:chemistry*; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D008567:Membranes, Artificial; D053768:Metal Nanoparticles / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*
chemical_list: D000814:Aniline Compounds; D006454:Hemoglobins; D008567:Membranes, Artificial; D037742:Nanotubes, Carbon; C416807:polyaniline; D020106:Acrylamide; D003300:Copper