title: Super-resolution fluorescence imaging with blink microscopy.
abstract: Recently, a new approach for super-resolution microscopy has emerged which is based on the successive localization of single molecules. The majority of molecules are prepared to reside in a nonfluorescent dark state, leaving only a few single molecules fluorescing. The single molecules can subsequently be localized on the camera image. Successive localization of all molecules allows reconstruction of a super-resolved image of the labeled structure. A variety of ways for limiting the number of locatable molecules have been developed recently which expand this current field of imaging. Here we describe a super-resolution microscopy method that employs the use of reversible, generic dark states, for example radical ion states. This method requires only a single laser source and can be carried out with many fluorescent dyes, in some cases, even in living cells. We provide a step-by-step procedure for this method, which we have called Blink Microscopy.
authors.affiliation: Institut für Physikalische und Theoretische Chemie-NanoBioScience, Braunschweig University of Technology, Braunschweig, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Steinhauer
authors.forename: Michelle S
authors.initials: MS
authors.lastname: Itano
authors.forename: Philip
authors.initials: P
authors.lastname: Tinnefeld
mesh_terms: D000199:Actins / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional / Q000379:methods*; D037181:Lectins, C-Type / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D055620:Optical Phenomena; D011956:Receptors, Cell Surface / Q000378:metabolism; D013223:Statistics as Topic; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D000199:Actins; D015815:Cell Adhesion Molecules; D005456:Fluorescent Dyes; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin