title: IL-17A levels increase in the infarcted region of the left ventricle in a rat model of myocardial infarction.
abstract: Th17 cells, a recently described subtype of CD4+ effector lymphocytes, have been linked to cell-mediated autoimmune and inflammatory diseases as well as to cardiovascular diseases. However, the participation of IL-17A in myocardial ischemic injury has not been clearly defined. We therefore conducted the present study to evaluate IL-17A and Th17-related cytokine levels in a rat model of myocardial infarction (MI). MI was induced in male Sprague Dawley rats by coronary artery ligation. Controls were sham-operated (Sh) or non-operated (C). Blood and samples from the left ventricle (LV) were collected at weeks 1 and 4 post-MI. At week 1, MI animals exhibited increased IL-6, IL-23 and TGF-β mRNA levels with no apparent change in IL-17 mRNA or protein levels in whole LV. Only TGF-β mRNA remained elevated at week 4 post-MI. However, further analysis revealed that IL-17A mRNA and protein levels as well as IL-6 and IL-23 mRNA were indeed increased in the infarcted region, though not in the remote non infarcted region of the LV, except for IL-23 mRNA. The increased expression of IL-17A and Th17-related cytokines in the infarcted region of LV, suggests that this proinflammatory pathway might play a role in early stages of post MI cardiac remodelling.
authors.affiliation: Laboratorio de Inmunología Celular y Molecular, Facultad de Medicina, Universidad de los Andes, Santiago, Chile.
authors.forename: Ana María
authors.initials: AM
authors.lastname: Ávalos
authors.forename: Felipe A
authors.initials: FA
authors.lastname: Apablaza
authors.forename: Mariana
authors.initials: M
authors.lastname: Quiroz
authors.forename: Viviana
authors.initials: V
authors.lastname: Toledo
authors.forename: Juan Pedro
authors.initials: JP
authors.lastname: Peña
authors.forename: Luis
authors.initials: L
authors.lastname: Michea
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Irarrázabal
authors.forename: Flavio A
authors.initials: FA
authors.lastname: Carrión
authors.forename: Fernando E
authors.initials: FE
authors.lastname: Figueroa
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006352:Heart Ventricles / Q000378:metabolism*; D020381:Interleukin-17 / Q000378:metabolism*; D008297:Male; D009203:Myocardial Infarction / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D058504:Th17 Cells / Q000378:metabolism*
chemical_list: D020381:Interleukin-17; D012333:RNA, Messenger