title: MicroRNA 497 modulates interleukin 1 signalling via the MAPK/ERK pathway.
abstract: The MAPK/ERK signalling pathway has been described to mediate IL-1 induction of target genes and is known to be regulated by microRNAs (miRNA). We describe a novel miRNA regulating the expression of the MEK1 gene and how it impacts IL-1 induced IL-6 transcription. miR-497 was predicted to target MEK1 3'UTR using bioinformatic tools. Transfection of miR-497 into HeLa cells inhibited MEK1 protein expression by 50%. In transient transfection experiments, the luciferase activity of a MEK1 3'UTR luciferase reporter construct was reduced in the presence of miR-497, and mutation of the predicted miR-497 binding site restored activity. miR-497 also decreased protein levels of RAF1 and ERK1 but not ERK2. Addition of miR-497 was further shown to inhibit IL-1 induced IL-6 gene transcription.
authors.affiliation: Division of Infection and Immunity, University College London, 5 University Street, London WC1E 6JF, UK.
authors.forename: Dongling
authors.initials: D
authors.lastname: Zheng
authors.forename: Anna
authors.initials: A
authors.lastname: Radziszewska
authors.forename: Patricia
authors.initials: P
authors.lastname: Woo
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000494:pharmacology*; D015850:Interleukin-6 / Q000235:genetics*; D048369:MAP Kinase Kinase 1 / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics* / Q000502:physiology
chemical_list: D020413:3' Untranslated Regions; D007375:Interleukin-1; D053583:Interleukin-1beta; D015850:Interleukin-6; D035683:MicroRNAs; D048369:MAP Kinase Kinase 1; C482125:MAP2K1 protein, human