title: Protease-catalysed direct asymmetric Mannich reaction in organic solvent.
abstract: We reported the first enzyme-catalysed, direct, three-component asymmetric Mannich reaction using protease type XIV from Streptomyces griseus (SGP) in acetonitrile. Yields of up to 92% with enantioselectivities of up to 88% e.e. and diastereoselectivities of up to 92:8 (syn:anti) were achieved under the optimised conditions. This enzyme's catalytic promiscuity expands the application of this biocatalyst and provides a potential alternative method for asymmetric Mannich reactions.
authors.affiliation: School of Chemistry and Chemical Engineering, Southwest University , Chongqing, 400715, P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xue
authors.forename: Ling-Po
authors.initials: LP
authors.lastname: Li
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: He
authors.forename: Zhi
authors.initials: Z
authors.lastname: Guan
mesh_terms: D000097:Acetonitriles; D002384:Catalysis; D008352:Mannich Bases / Q000737:chemistry* / Q000378:metabolism*; D015394:Molecular Structure; D011402:Pronase / Q000378:metabolism*; D012997:Solvents; D013237:Stereoisomerism; D013305:Streptomyces griseus / Q000201:enzymology; D013379:Substrate Specificity
chemical_list: D000097:Acetonitriles; D008352:Mannich Bases; D012997:Solvents; D011402:Pronase; C032159:acetonitrile