title: Efficiency of biolistic DNA vaccination in experimental type I allergy.
abstract: Gene gun-mediated delivery of allergen-encoding plasmid DNA has been in focus for many years now as being a needle-free alternative to the protein-based desensitization regimen used in specific immunotherapy. Biolistic immunization with the Helios gene gun has proven to be potent in the induction of antigen-specific CD4(+) and CD8(+) T cells. Here we describe biolistic vaccination in experimental mouse models of IgE-mediated type I allergy as well as allergen-induced airway inflammation.
authors.affiliation: Department of Dermatology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany. rakerv@uni-mainz.de
authors.forename: Verena
authors.initials: V
authors.lastname: Raker
authors.forename: Joachim
authors.initials: J
authors.lastname: Maxeiner
authors.forename: Angelika B
authors.initials: AB
authors.lastname: Reske-Kunz
authors.forename: Stephan
authors.initials: S
authors.lastname: Sudowe
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D000918:Antibody Specificity; D019470:Biolistics / Q000295:instrumentation*; D018893:Bronchoalveolar Lavage; D005260:Female; D006967:Hypersensitivity / Q000235:genetics / Q000276:immunology / Q000503:physiopathology / Q000517:prevention & control*; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D008168:Lung / Q000276:immunology / Q000503:physiopathology; D051379:Mice; D008807:Mice, Inbred BALB C; D012140:Respiratory Tract Diseases / Q000235:genetics / Q000276:immunology / Q000503:physiopathology / Q000517:prevention & control; D014162:Transfection / Q000295:instrumentation; D014611:Vaccination / Q000295:instrumentation*; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*; D001616:beta-Galactosidase / Q000276:immunology
chemical_list: D000485:Allergens; D019444:Vaccines, DNA; D007073:Immunoglobulin E; D001616:beta-Galactosidase