title: FISH analysis using PPAR γ-specific probes for detection of PAX8-PPAR γ translocation in follicular thyroid neoplasms.
abstract: Fluorescence in situ hybridization (FISH) is increasingly gaining importance in clinical diagnostics settings. Due to the ability of the technique to detect chromosomal abnormalities in samples with low cellularity or containing a mixed population of cells even at a single-cell level, it has become more popular in cancer research and diagnosis. Here, we describe the FISH technique for detection of PAX8-PPARγ translocation in follicular thyroid neoplasms, and the optimal protocol for the detection of this fusion gene using in archival formalin-fixed paraffin-embedded (FFPE) thyroid tissue sections.
authors.affiliation: Department of Pathology, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Kuala Lumpur, Malaysia, sharifah@ppukm.ukm.my.
authors.forename: Noor Akmal Syed Husain
authors.initials: NA
authors.lastname: Sharifah
authors.forename: Zubaidah
authors.initials: Z
authors.lastname: Zakaria
authors.forename: Wai Kit
authors.initials: WK
authors.lastname: Chia
mesh_terms: D018263:Adenocarcinoma, Follicular / Q000378:metabolism* / Q000473:pathology; D011232:Chemical Precipitation; D015342:DNA Probes / Q000737:chemistry / Q000378:metabolism*; D005374:Filtration; D005456:Fluorescent Dyes / Q000737:chemistry; D005557:Formaldehyde / Q000378:metabolism; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007211:Indoles / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009691:Nucleic Acid Denaturation; D000072636:PAX8 Transcription Factor; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D051761:Paired Box Transcription Factors / Q000235:genetics*; D016612:Paraffin Embedding; D010434:Pepsin A / Q000378:metabolism; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016707:Tissue Fixation
chemical_list: D015342:DNA Probes; D005456:Fluorescent Dyes; D007211:Indoles; D000072636:PAX8 Transcription Factor; C083325:PAX8 protein, human; D047495:PPAR gamma; D051761:Paired Box Transcription Factors; D011993:Recombinant Fusion Proteins; D005557:Formaldehyde; C007293:DAPI; D010434:Pepsin A