title: Far-infrared-assisted preparation of a graphene-nickel nanoparticle hybrid for the enrichment of proteins and peptides.
abstract: A new approach based on far infrared-assisted in situ reduction was developed for the facile one-step preparation of graphene-nickel nanoparticle hybrid by refluxing a mixture solution containing graphene oxide, nickel(II) sulfate, and hydrazine over an far-infrared heater. The reduction time was as short as 20 min. The structure of the material was investigated by transmission electron microscopy, scanning electron microscopy, X-ray diffraction, energy dispersive spectroscopy, vibrating sample magnetometery, and Fourier transform infrared spectroscopy. Magnetic investigations indicate that the grapheme-nickel nanoparticle hybrid exhibits ferromagnetic behavior at room temperature. Meanwhile, the hybrid was successfully employed in the enrichment and identification of proteins and peptides in combination with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry based on its excellent magnetic responsibility, high dispersibility, large surface area, and hydrophobicity, indicating great promise for a wide range of applications.
authors.affiliation: Key Laboratory of Public Health and Safety, Ministry of Education, School of Public Health, Fudan University, Shanghai, PR China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Qu
authors.forename: Huimin
authors.initials: H
authors.lastname: Bao
authors.forename: Luyan
authors.initials: L
authors.lastname: Zhang
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
mesh_terms: D006108:Graphite / Q000737:chemistry*; D006834:Hydrazines / Q000737:chemistry*; D013058:Mass Spectrometry; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D009532:Nickel / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D006834:Hydrazines; D010455:Peptides; D011506:Proteins; C029424:hydrazine; D006108:Graphite; D009532:Nickel