title: A proteomic study of potential VEGF-C-associated proteins in bladder cancer T24 cells.
abstract: BACKGROUND
  Overexpression of vascular endothelial growth factor-C (VEGF-C) has been found to play an important role in malignant progression of various cancer cells, in addition to lymphangiogenesis. However, the mechanisms involved are still largely unknown. Our early research has confirmed that the expression of VEGF-C in bladder cancer was markedly higher than that in normal bladder tissues. VEGF-C can also obviously promote proliferation and invasion of bladder cancer T24 cells. In the present work, we attempted to use proteomic analysis to screen out potential VEGF-C-associated proteins involved in malignant progression of the bladder cancer T24 cells.
  
  
  METHODS
  Lentivirus vector-based RNA interference (RNAi) was employed to diminish VEGF-C expression of bladder cancer T24 cells. Then we performed comparative proteome analysis to explore differentially expressed proteins in T24 cells with and without VEGF-C siRNA, by two-dimensional difference gel electrophoresis (2D-DIGE).
  
  
  RESULTS
  Twenty-three proteins were identified. Some proteins (matrix metalloproteinase-9, Keratin 8, Serpin B5, Annexin A8) with significant differences were further confirmed by Western blotting.
  
  
  CONCLUSIONS
  The 23 potential VEGF-C-associated proteins identified in our study provide us with further insights into the mechanism of VEGF-C promoting malignant progression of bladder cancer cells.
authors.affiliation: Department of Urology, First Affiliated Hospital of Nanhua University, Hengyang city, China.
authors.forename: Hui-hui
authors.initials: HH
authors.lastname: Zhang
authors.forename: Fan
authors.initials: F
authors.lastname: Qi
authors.forename: Xiong-bing
authors.initials: XB
authors.lastname: Zu
authors.forename: You-han
authors.initials: YH
authors.lastname: Cao
authors.forename: Jian-guang
authors.initials: JG
authors.lastname: Miao
authors.forename: Liang
authors.initials: L
authors.lastname: Xu
authors.forename: Lin
authors.initials: L
authors.lastname: Qi
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D034741:RNA, Small Interfering / Q000378:metabolism; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D057825:Two-Dimensional Difference Gel Electrophoresis; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000473:pathology; D042582:Vascular Endothelial Growth Factor C / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D009363:Neoplasm Proteins; D034741:RNA, Small Interfering; C467486:VEGFC protein, human; D042582:Vascular Endothelial Growth Factor C; D020780:Matrix Metalloproteinase 9