title: Bauhinia forficata Link authenticity using flavonoids profile: relation with their biological properties.
abstract: HPLC-DAD-ESI/MS(n) was used to ascertain the authenticity of two certified and two commercial Bauhinia forficata Link samples. Different flavonoids profiles were obtained, involving 39 compounds. Just kaempferol-3-O-(2-rhamnosyl)rutinoside was found in all analysed samples. Five compounds were common to the certified samples of B. forficata Link and B. forficata Link subsp. pruinosa (Vogel) Fortunato & Wunderlin, being kaempferol derivatives the most representative ones. The phenolic composition of B. forficata Link subsp. pruinosa (Vogel) Fortunato & Wunderlin is described herein for the first time, accounting for eight compounds, while 10 new compounds were identified in B. forficata Link. Commercial B. forficata Link showed higher contents of quercetin derivatives, in addition to the presence of myricetin derivatives and flavonoids-(galloyl)glycosides, for which the MS fragmentation pattern is reported for the first time. B. forficata Link and the two commercial samples were able to inhibit α-glucosidase, with EC(50) values lower than that found for acarbose. Mild effects on cholinesterases were observed with the certified samples, while commercial ones were more effective. The same behaviour was observed concerning the scavenging of DPPH, nitric oxide and superoxide radicals. The presence of high contents of quercetin derivatives in commercial samples seems to directly influence their biological properties. The differences between phenolic profiles and their relation with the authenticity of commercial samples are discussed.
authors.affiliation: Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus University Espinardo, Murcia, Spain. federico@cebas.csic.es
authors.forename: Federico
authors.initials: F
authors.lastname: Ferreres
authors.forename: Angel
authors.initials: A
authors.lastname: Gil-Izquierdo
authors.forename: Juliana
authors.initials: J
authors.lastname: Vinholes
authors.forename: Sara T
authors.initials: ST
authors.lastname: Silva
authors.forename: Patrícia
authors.initials: P
authors.lastname: Valentão
authors.forename: Paula B
authors.initials: PB
authors.lastname: Andrade
mesh_terms: D036481:Bauhinia / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis* / Q000378:metabolism; D005506:Food Contamination / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis* / Q000378:metabolism; D011786:Quality Control; D011794:Quercetin / Q000032:analysis / Q000378:metabolism
chemical_list: D005419:Flavonoids; D010936:Plant Extracts; D011794:Quercetin