title: PPAR-alpha cloning, expression, and characterization.
abstract: Peroxisome proliferator-activated receptor α (PPARα) is a member of the nuclear/steroid receptor gene superfamily that also comprises β, δ, and γ isoforms. PPARα is a ligand-activated transcription factor that plays an important role in the regulation of many genes involved in key metabolic processes. Today, PPARα has been cloned from mammalian, marsupial, and a number of marine species and its expression has been found to be relatively tissue- and species-specific. Here, we describe the methods for cloning of PPARα genes by RT-PCR and RACE approaches and related protocols for studying the expression of cloned PPARα cDNAs in mammalian cell systems.
authors.affiliation: The University of Adelaide, Roseworthy, SA, Australia. suong.ngo@.adelaide.edu.au
authors.forename: Suong N T
authors.initials: SN
authors.lastname: Ngo
authors.forename: Ross A
authors.initials: RA
authors.lastname: McKinnon
mesh_terms: D000818:Animals; D019556:COS Cells; D002467:Cell Nucleus / Q000378:metabolism; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular / Q000379:methods*; D003600:Cytosol / Q000378:metabolism; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D047493:PPAR alpha / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017421:Sequence Analysis
chemical_list: D018076:DNA, Complementary; D047493:PPAR alpha; D012333:RNA, Messenger