title: Low cost, disposable biosensors allow detection of antibodies with results equivalent to ELISA in 15 min.
abstract: An assay for detection of antibodies to bovine herpesvirus-1 (BoHV-1) was developed by combining a commercial low cost, disposable biosensor system (Vantix™) and reagents from an established Enzyme-Linked Immunosorbent Assay (ELISA). The biosensor assay produced equivalent results to ELISA within 15 min when testing 194 bovine serum and 50 bulk milk samples submitted for routine testing. The biosensor assay can provide quantitative analysis demonstrated by measuring the level of antibody in milk samples. The results of this study suggest that Vantix™ is a promising platform for routine immunological testing. The technology may be particularly useful for low to medium throughput tests where rapid results are required. The biosensors could also form the basis of a future point-of-care test platform.
authors.affiliation: Technology Transfer Unit, Specialist Scientific Services Department, Animal Health and Veterinary Laboratories Agency - Weybridge, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Cork
authors.forename: Rebecca M
authors.initials: RM
authors.lastname: Jones
authors.forename: Jason
authors.initials: J
authors.lastname: Sawyer
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000097:blood / Q000276:immunology*; D015374:Biosensing Techniques / Q000191:economics / Q000379:methods*; D002417:Cattle; D003362:Cost-Benefit Analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000191:economics / Q000379:methods*; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D007241:Infectious Bovine Rhinotracheitis / Q000175:diagnosis / Q000276:immunology* / Q000821:virology; D008892:Milk / Q000276:immunology / Q000821:virology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000914:Antibodies, Viral