title: Development of a biochemical oxygen demand sensor using gold-modified boron doped diamond electrodes.
abstract: Gold-modified boron doped diamond (BDD) electrodes were examined for the amperometric detection of oxygen as well as a detector for measuring biochemical oxygen demand (BOD) using Rhodotorula mucilaginosa UICC Y-181. An optimum potential of -0.5 V (vs Ag/AgCl) was applied, and the optimum waiting time was observed to be 20 min. A linear calibration curve for oxygen reduction was achieved with a sensitivity of 1.4 μA mg(-1) L oxygen. Furthermore, a linear calibration curve in the glucose concentration range of 0.1-0.5 mM (equivalent to 10-50 mg L(-1) BOD) was obtained with an estimated detection limit of 4 mg L(-1) BOD. Excellent reproducibility of the BOD sensor was shown with an RSD of 0.9%. Moreover, the BOD sensor showed good tolerance against the presence of copper ions up to a maximum concentration of 0.80 μM (equivalent to 50 ppb). The sensor was applied to BOD measurements of the water from a lake at the University of Indonesia in Jakarta, Indonesia, with results comparable to those made using a standard method for BOD measurement.
authors.affiliation: Department of Chemistry, Faculty of Mathematics and Science, University of Indonesia, Kampus UI Depok, Jakarta 16424, Indonesia.
authors.forename: Tribidasari A
authors.initials: TA
authors.lastname: Ivandini
authors.forename: Endang
authors.initials: E
authors.lastname: Saepudin
authors.forename: Habibah
authors.initials: H
authors.lastname: Wardah
authors.lastname: Harmesa
authors.forename: Netra
authors.initials: N
authors.lastname: Dewangga
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Einaga
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001895:Boron / Q000737:chemistry*; D018130:Diamond / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D010100:Oxygen / Q000378:metabolism*; D012248:Rhodotorula / Q000378:metabolism
chemical_list: D006046:Gold; D018130:Diamond; D001895:Boron; D010100:Oxygen