title: Characterization of 39 novel EST-SSR markers for Liriodendron tulipifera and cross-species amplification in L. chinense (Magnoliaceae).
abstract: OBJECTIVE
  A novel set of simple sequence repeat (SSR) markers were developed and characterized from the expressed sequence tag (EST) database of Liriodendron tulipifera for application in population genetic studies of Liriodendron.
  
  
  RESULTS
  Thirty-nine polymorphic EST-SSR loci were identified among 27 individuals sampled from a cultivated population of L. tulipifera. The number of alleles per locus ranged from three to 18. The average observed heterozygosity and expected heterozygosity were 0.684 and 0.778, respectively. Of the 39 loci, 32 showed interspecific transferability and polymorphism in a related species, L. chinense. The number of alleles per locus ranged from two to 11, and the average observed heterozygosity and expected heterozygosity were 0.475 and 0.736, respectively.
  
  
  CONCLUSIONS
  The developed EST-SSR markers will be useful for investigating adaptive genetic differentiation in Liriodendron.
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Speciality Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, Hubei, People's Republic of China.
authors.forename: Ai-Hong
authors.initials: AH
authors.lastname: Yang
authors.forename: Jin-Ju
authors.initials: JJ
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Tian
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Yao
mesh_terms: D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D020224:Expressed Sequence Tags; D005838:Genotype; D015810:Linkage Disequilibrium; D031567:Liriodendron / Q000145:classification / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant