title: Unravelling the retention of proliferation and differentiation potency in extensive culture of human subcutaneous fat-derived mesenchymal stem cells in different media.
abstract: OBJECTIVE
  This study has intended to investigate longevity of subcutaneous fat-derived mesenchymal stem cells (SF-MSCs) under extensive culturing. It has also focused on optimization of culture media for them over prolonged periods in vitro.
  
  
  METHODS
  We evaluated SF-MSCs with reference to phenotypic characterization, proliferative ability, karyotype stability and differentiation potency with early (P3) and late passage (P20) conditions, using four different media, DMEM-LG, ALPHA-MEM, DMEM-F12 and DMEM-KO.
  
  
  RESULTS
  This study unravels retention of SF-MSC characteristics in facets of phenotypic expression profile (CD 90, CD 105, CD 73, CD 34, CD 29, CD 54, CD 49d, CD 117, HLA-DR, CD 166, CD 31, CD 44), proliferative characteristics, karyotyping and differentiation potency prolonged culturing to P25 in all media. Population doubling time (PDT) in Alpha MEM, DMEM LG, DMEM F 12, DMEM KO were identified to be (1.81, 1.84, 1.9, 2.08 days) at early passage and (2.93, 2.94, 3.12, 3.06 days) at late passage. As a corollary, Alpha MEM and DMEM LG serve as appropriate basal media for SF-MSC when proliferative potency is considered.
  
  
  CONCLUSIONS
  In research, it is imperative that SF-MSC uphold their expansion potency in the aforesaid attributes in all media over extensive culturing, thereby transforming their colossal in vitro potency, with the aim of curing a wide horizon of diseases.
authors.affiliation: Lifeline Multispeciality Hospitals, Chennai, India.
authors.forename: M
authors.initials: M
authors.lastname: Dhanasekaran
authors.forename: S
authors.initials: S
authors.lastname: Indumathi
authors.forename: M
authors.initials: M
authors.lastname: Rashmi
authors.forename: J S
authors.initials: JS
authors.lastname: Rajkumar
authors.forename: D
authors.initials: D
authors.lastname: Sudarsanam
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D000090266:Chromobox Protein Homolog 5; D003470:Culture Media / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology; D008875:Middle Aged; D009930:Organic Chemicals / Q000378:metabolism; D050151:Subcutaneous Fat / Q000166:cytology*
chemical_list: D015703:Antigens, CD; C000715594:CBX5 protein, human; D003470:Culture Media; D009930:Organic Chemicals; C420642:alpha minimal essential medium; D000090266:Chromobox Protein Homolog 5