title: Neuronal differentiation of human iPS-cells in a rat cortical primary culture.
abstract: We tested the neuronal differentiation of human iPS-cells under in vitro conditions. For this purpose we pre-differentiated human (h) iPS-cells into neural stem cells and co-cultivated them with a cortical primary culture from embryonic rats. After 2 days of co-cultivation a certain number of hiPS-cells exhibited a clear neuronal morphology combined with expression of betaIII-tubulin and doublecortin. In addition, we found hiPS-cells without neuronal differentiation and cells already expressing betaIII-tubulin but not having yet distinctive axonal and dendritic processes. Human neuronal progenitors, starting neuronal differentiation, were contacted by both neuronal processes from rat neurons and oligodendrocytes, indicating a possible instructive influence by the primary culture on human cells. After 7 days of co-cultivation, however, we observed a complete degeneration of human iPS-derived cells and phagocytosis by microglial cells. Immunocytochemical stainings surprisingly revealed that microglial cells of the cortical primary culture express both CD8 and T-cell receptors.
authors.affiliation: German Center for Neurodegenerative Diseases (DZNE), Magdeburg, Germany. holger.braun@ifn-magdeburg.de.
authors.forename: Holger
authors.initials: H
authors.lastname: Braun
authors.forename: Anja
authors.initials: A
authors.lastname: Günther-Kern
authors.forename: Klaus
authors.initials: K
authors.lastname: Reymann
authors.forename: Brigitte
authors.initials: B
authors.lastname: Onteniente
mesh_terms: D000818:Animals; D000941:Antigens / Q000378:metabolism; D039481:CD11b Antigen / Q000378:metabolism; D002135:Calcium-Binding Proteins; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D018920:Coculture Techniques; D004268:DNA-Binding Proteins / Q000378:metabolism; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D004622:Embryo, Mammalian; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000502:physiology*; D008840:Microfilament Proteins; D017628:Microglia / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D009474:Neurons / Q000502:physiology*; D009479:Neuropeptides / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D009836:Oligodendroglia / Q000502:physiology; D010587:Phagocytosis / Q000502:physiology; D011509:Proteoglycans / Q000378:metabolism; D051381:Rats; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D014404:Tubulin / Q000378:metabolism; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000941:Antigens; D039481:CD11b Antigen; D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D008840:Microfilament Proteins; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D050814:Octamer Transcription Factor-3; D011509:Proteoglycans; D011948:Receptors, Antigen, T-Cell; D055748:SOXB1 Transcription Factors; D014404:Tubulin; D000099216:Chondroitin Sulfate Proteoglycan 4; C112986:AIF1 protein, human; C000714848:Dcx protein, rat; C494173:POU5F1 protein, human; C527782:SOX2 protein, human