title: In vitro assessment of mesenchymal stem cells immunosuppressive potential in multiple sclerosis patients.
abstract: Mesenchymal stem cells (MSC) are promising for multiple sclerosis (MS) treatment. However, clinical results remain controversial, and no criteria are available for predicting the efficiency of MSC therapy. Using an in vitro model of lymphocytes and MSC cocultivation we revealed that the Index of MSC Suppression of myelin-induced memory T cells proliferation was stronger than that of PHA-stimulated proliferation and inversely correlated with patients'EDSS score. In vitro expression of CD119 (IFNGR1) in mitogen/myelin-stimulated T cells increased in the presence of MSC being inversely correlated with T-lymphocytes proliferation. The Index of MSC Suppression and CD119 expression in T-lymphocytes may be useful when assessing MSC immunosuppressive potential in MS patients.
authors.affiliation: Belarusian Medical Academy of Post-Graduate Education, 3, Brovki str., build. 3, Minsk 220013, Belarus. zafranskaya@gmail.com
authors.forename: Marina M
authors.initials: MM
authors.lastname: Zafranskaya
authors.forename: Darya B
authors.initials: DB
authors.lastname: Nizheharodova
authors.forename: Mariya Yu
authors.initials: MY
authors.lastname: Yurkevich
authors.forename: Natallia V
authors.initials: NV
authors.lastname: Lamouskaya
authors.forename: Yana M
authors.initials: YM
authors.lastname: Motuzova
authors.forename: Svetlana S
authors.initials: SS
authors.lastname: Bagatka
authors.forename: Halina I
authors.initials: HI
authors.lastname: Ivanchik
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Fedulov
mesh_terms: D000328:Adult; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D007108:Immune Tolerance; D008297:Male; D059630:Mesenchymal Stem Cells / Q000276:immunology* / Q000473:pathology; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000276:immunology* / Q000473:pathology; D017471:Receptors, Interferon / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D000096743:Interferon gamma Receptor
chemical_list: D017471:Receptors, Interferon; D000096743:Interferon gamma Receptor