title: A novel, disposable, screen-printed amperometric biosensor for ketone 3-β-hydroxybutyrate fabricated using a 3-β-hydroxybutyrate dehydrogenase-mesoporous silica conjugate.
abstract: A disposable amperometric biosensor for ketone 3-β-hydroxybutyrate (3HB) has been developed successfully. The sensor is based on a screen-printed carbon electrode containing Meldola's Blue (MB) and sensing components containing nicotinamide adenine dinucleotide (NAD(+)) and 3-β-hydroxybutyrate dehydrogenase (3HBDH) immobilized in mesoporous silica (FSM8.0) using an aqueous photo-cross-linkable polymer matrix of polyvinyl alcohol (O-391), and it requires only a small sample volume of 10 μL for the measurement. The behavior of a resulting biosensor, i.e., 3HBDH-FSM8.0/NAD(+)/MB-SPCE, was examined in terms of NAD(+) concentration for construction, pH, applied potential, operational range, selectivity, and storage stability. The sensor showed an optimum response at a pH of 7.6 and at an applied potential of -50 mV. The determination range and the response time for 3HB were from 30 μM to 8 mM and approximately 30 s, respectively. In addition, the sensor was quite stable and maintained >90% of its initial response after being stored for over 6 months. This result implies that our method provides a novel biosensor for ketone 3-β-hydroxybutyrate which is easy-to-use, cost-effective, and has good reproducibility, which are vital for commercial purposes.
authors.affiliation: Funai Electric Advanced Applied Technology Research Institute Inc. (FEAT), TCI 37A, 2-1-6, Sengen, Tsukuba-shi, Ibaraki 305-0047, Japan. shimomura.t@funai-atri.co.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Shimomura
authors.forename: Touru
authors.initials: T
authors.lastname: Sumiya
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Ono
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Ito
authors.forename: Taka-aki
authors.initials: TA
authors.lastname: Hanaoka
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000737:chemistry*; D015374:Biosensing Techniques; D002138:Calibration; D002244:Carbon / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006884:Hydroxybutyrate Dehydrogenase / Q000737:chemistry*; D007659:Ketones / Q000737:chemistry*; D008956:Models, Chemical; D008968:Molecular Conformation; D011108:Polymers / Q000737:chemistry; D011208:Powders; D012825:Silicon / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D007659:Ketones; D011108:Polymers; D011208:Powders; D002244:Carbon; D012822:Silicon Dioxide; D006884:Hydroxybutyrate Dehydrogenase; D020155:3-Hydroxybutyric Acid; D012825:Silicon