title: Toxin-antigen conjugates as selection tools for antibody producing cells.
abstract: The generation of antibodies with designated specificity requires cost-intensive and time-consuming screening procedures. Here we present a new method by which hybridoma cells can be selected based on the specificity of the produced antibody by the use of antigen-toxin-conjugates thus eliminating the need of a screening procedure. Initial experiments were done with methotrexate as low molecular weight toxin and fluorescein as model antigen. Methotrexate and a methotrexate-fluorescein conjugate were characterized regarding their toxicity. Afterwards the effect of the fluorescein-specific antibody B13-DE1 on the toxicity of the methotrexate-fluorescein conjugate was determined. Finally, first results showed that hybridoma cells that produce fluorescein specific antibodies are able to grow in the presence of fluorescein-toxin-conjugates.
authors.affiliation: Junior Research Group Antibody Technologies, Department of Biotechnology, Institute of Biochemistry and Biology, Potsdam University, Karl-Liebknecht-Str 24-25, D-14476 Potsdam-Golm, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Messerschmidt
authors.forename: Katja
authors.initials: K
authors.lastname: Heilmann
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D000921:Antibody-Producing Cells / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D000941:Antigens / Q000737:chemistry / Q000276:immunology* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D005452:Fluoresceins / Q000494:pharmacology; D006825:Hybridomas / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D007158:Immunologic Techniques / Q000379:methods; D000922:Immunotoxins / Q000737:chemistry / Q000276:immunology* / Q000494:pharmacology; D008727:Methotrexate / Q000031:analogs & derivatives / Q000276:immunology / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000737:chemistry / Q000276:immunology; D015203:Reproducibility of Results
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D000941:Antigens; D005452:Fluoresceins; D000922:Immunotoxins; C103864:fluorescein-methotrexate; D010047:Ovalbumin; D008727:Methotrexate