title: Photocleavable DNA barcode-antibody conjugates allow sensitive and multiplexed protein analysis in single cells.
abstract: DNA barcoding is an attractive technology, as it allows sensitive and multiplexed target analysis. However, DNA barcoding of cellular proteins remains challenging, primarily because barcode amplification and readout techniques are often incompatible with the cellular microenvironment. Here we describe the development and validation of a photocleavable DNA barcode-antibody conjugate method for rapid, quantitative, and multiplexed detection of proteins in single live cells. Following target binding, this method allows DNA barcodes to be photoreleased in solution, enabling easy isolation, amplification, and readout. As a proof of principle, we demonstrate sensitive and multiplexed detection of protein biomarkers in a variety of cancer cells.
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital/Harvard Medical, Boston, 02114, United States.
authors.forename: Sarit S
authors.initials: SS
authors.lastname: Agasti
authors.forename: Monty
authors.initials: M
authors.lastname: Liong
authors.forename: Vanessa M
authors.initials: VM
authors.lastname: Peterson
authors.forename: Hakho
authors.initials: H
authors.lastname: Lee
authors.forename: Ralph
authors.initials: R
authors.lastname: Weissleder
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D045744:Cell Line, Tumor; D058893:DNA Barcoding, Taxonomic; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D051379:Mice; D055668:Photochemical Processes; D011506:Proteins / Q000032:analysis*
chemical_list: D000906:Antibodies; D005456:Fluorescent Dyes; D011506:Proteins