title: Genomic profiling of a human leukemic monocytic cell-line (THP-1) exposed to alpha particle radiation.
abstract: This study examined alpha (α-) particle radiation effects on global changes in gene expression in human leukemic monocytic cells (THP-1) for the purposes of mining for candidate biomarkers that could be used for the development of a biological assessment tool. THP-1 cells were exposed to α-particle radiation at a dose range of 0 to 1.5 Gy. Twenty-four hours and three days after exposure gene expression was monitored using microarray technology. A total of 16 genes were dose responsive and classified as early onset due to their expression 24 h after exposure. Forty-eight transcripts were dose responsive and classified as late-onset as they were expressed 72 h after exposure. Among these genes, 6 genes were time and dose responsive and validated further using alternate technology. These transcripts were upregulated and associated with biological processes related to immune function, organelle stability and cell signalling/communication. This panel of genes merits further validation to determine if they are strong candidate biomarkers indicative of α-particle exposure.
authors.affiliation: Consumer and Clinical Radiation Protection Bureau, Health Canada, Healthy Environment and Consumer Safety Branch, Ottawa, ON, Canada K1A 0K9. vinita chauhan@hc-sc.gc.ca
authors.forename: Vinita
authors.initials: V
authors.lastname: Chauhan
authors.forename: Matthew
authors.initials: M
authors.lastname: Howland
mesh_terms: D000512:Alpha Particles; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects*; D006801:Humans; D007948:Leukemia, Monocytic, Acute / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy*; D008297:Male; D009000:Monocytes / Q000473:pathology / Q000528:radiation effects*