title: [Glycerol metabolism in surfactants producers Acinetobacter calcaaceticus IMV B-7241 and Rhodococcus erythropolis IMV Ac-5017].
abstract: Key enzymes of glycerol metabolism were detected in the cells of surfactants producers Rhodococcus erythropolis IMV Ac-5017 and Acinetobacter calcaaceticus IMV B-7241 grown on glycerol. It has been established that in the both strains glycerol catabolism to dihydroxyacetonephosphate (the intermediate of glycolysis) may be performed in two ways: through glycerol-3-phosphate (glycerol kinase activity 740-840 nmol min(-1) mg(-1) of protein) and through dihydroxyacetone. Glycerol oxidation to dihydroxyacetone in the strains IMV B-7241 and Ac-5017 is catalised by pyrrholo-quinolinquinone-dependent glycerol dehydrogenases and nitroso-N,N-dimethylaniline-dependent alcohol dehydrogenases. Both glyoxylate cycle and phosphoenol pyruvate(PEP)-carboxylase function as anaplerotic paths in R. erythropolis IMV Ac-5017, and only PEP-carboxylase reaction (1045 +/- 52 nmol min(-1) mg(-1) of proteins) functions in A. calcoaceticus IMV B-7241. The data obtained serve as the basis for theoretical calculations of optimal molar ratio of concentrations of energetically nonequivalent substrates for intensifying the surfactants synthesis on their mixture.
authors.forename: T P
authors.initials: TP
authors.lastname: Pirog
authors.forename: T A
authors.initials: TA
authors.lastname: Shevchuk
authors.forename: M A
authors.initials: MA
authors.lastname: Shuliakova
mesh_terms: D000150:Acinetobacter / Q000378:metabolism*; D000426:Alcohol Dehydrogenase / Q000378:metabolism; D050260:Carbohydrate Metabolism; D003470:Culture Media; D004098:Dihydroxyacetone / Q000378:metabolism; D004099:Dihydroxyacetone Phosphate / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D005991:Glycerol Kinase / Q000378:metabolism; D010728:Phosphoenolpyruvate / Q000378:metabolism; D010730:Phosphoenolpyruvate Carboxylase / Q000378:metabolism; D012240:Rhodococcus / Q000378:metabolism*; D013045:Species Specificity; D013379:Substrate Specificity; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism; D013501:Surface-Active Agents / Q000378:metabolism*
chemical_list: D003470:Culture Media; D013501:Surface-Active Agents; D000431:Ethanol; D004099:Dihydroxyacetone Phosphate; D010728:Phosphoenolpyruvate; D013401:Sugar Alcohol Dehydrogenases; D000426:Alcohol Dehydrogenase; C020672:glycerol dehydrogenase; D005991:Glycerol Kinase; D010730:Phosphoenolpyruvate Carboxylase; D005947:Glucose; D004098:Dihydroxyacetone; D005990:Glycerol