title: Expressed sequence tags of Aspergillus fumigatus: Extension of catalogue and their evaluation as putative drug targets and/or diagnostic markers.
abstract: Aspergillus fumigatus a fungal pathogen is implicated in a spectrum of allergic and invasive disorders in humans. Validation of transcriptome of pathogen is essential for understanding its virulence mechanism and to identify new therapeutic targets/diagnostic markers. In order to rapidly identify genes of Aspergillus fumigatus we adopted sequencing of cDNA clones. Our earlier effort has lead to identification of 68 expressed sequence tags of Aspergillus fumigatus. Present study describes 52 more expressed sequence tags generated by sequencing 200 phage clones of a non-normalized cDNA library. One of the cDNA clones comprised of the complete coding region for tetratricopeptide repeat domain protein gene. Various homology search algorithms were employed to assign functions to expressed sequence tags coding for hypothetical proteins, and relevance of these expressed sequence tags or their protein products as drug targets/diagnostic markers was examined by searching for homologues in fungi and human.
authors.affiliation: Institute of Genomics and Integrative Biology, Mall road, Delhi, 110007 India  ; Department of Biosciences, Jamia Millia Islamia, New Delhi, 110025 India.
authors.forename: Santosh Kumar
authors.initials: SK
authors.lastname: Upadhyay
authors.forename: Jata
authors.initials: J
authors.lastname: Shankar
authors.forename: Yogendra
authors.initials: Y
authors.lastname: Singh
authors.forename: Seemi Farhat
authors.initials: SF
authors.lastname: Basir
authors.forename: Taruna
authors.initials: T
authors.lastname: Madan
authors.forename: P Usha
authors.initials: PU
authors.lastname: Sarma
keywords: Aspergillus fumigatus; Diagnosis; Drug target; Expressed sequence tags; Tetratricopeptide repeat domain