title: Isolation and characterization of  48  polymorphic microsatellite markers for the blood clam Scapharca  broughtonii (Arcidae).
abstract: Blood clams (Scapharca broughtonii) are widely  cultivated and consumed in noutheast Asia. Forty-eight polymorphic  microsatellite loci were developed for this clam using magnetic-bead  hybridization enrichment. The number of alleles per locus ranged from 2 to 14.  Polymorphism of these loci was assessed in 30 individuals from a population  collected from coastal areas of Qingdao, China. The values of observed  heterozygosity, expected heterozygosity and polymorphism information content  per locus ranged from 0.1034 to 0.9655, from 0.1831 to 0.9208, and from 0.1638  to 0.8964, respectively. Forty-three of 48 loci conformed to Hardy-Weinberg  equilibrium. These microsatellite loci would be useful for molecular genetic  breeding, population genetics, genome mapping, and other relevant research on S.  broughtonii.
authors.affiliation: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Qingdao, China.
authors.forename: J-T
authors.initials: JT
authors.lastname: Tian
authors.forename: Z-H
authors.initials: ZH
authors.lastname: Liu
authors.forename: L-Q
authors.initials: LQ
authors.lastname: Zhou
authors.forename: B
authors.initials: B
authors.lastname: Wu
authors.forename: P
authors.initials: P
authors.lastname: Liu
authors.forename: A-G
authors.initials: AG
authors.lastname: Yang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D056426:Genetic Loci; D006579:Heterozygote; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D049894:Scapharca / Q000235:genetics*; D017422:Sequence Analysis, DNA