title: Signal-amplified platform for electrochemical immunosensor based on TiO2 nanotube arrays using a HRP tagged antibody-Au nanoparticles as probe.
abstract: In this study, a novel signal-amplified electrochemical immunosensor was proposed by using TiO(2) nanotube (TiNT) arrays as the platform. Due to the distinct tubular features-large surface area, high pore volume and good electrochemical conductivity, the TiNT based electrodes exhibited excellent signal-amplified effects. gold nanoparticle (AuNP) was further utilized to bind horseradish peroxidase (HRP) tagged antibodies as recognition elements. Compared to the immunosensor based on either flat electrode, the immunosensors using TiNT layer as electrode showed higher amplified electrochemical signals from the catalytic reaction of HRP relative to hydrogen peroxide (H(2)O(2)). Under optimal conditions, the proposed immunosensor exhibited a good electrochemical behavior to antigen in a concentration range from 0.1 ng mL(-1) to 10(5) ng mL(-1) with a detection limit of 0.01 ng mL(-1). The results showed that the TiNT-based electrochemical immunosensing platform could provide a great potential in clinical application for detection of low-abundant proteins.
authors.affiliation: College of Sciences, Northeastern University, Shenyang 110004, China.
authors.forename: Zhi-Da
authors.initials: ZD
authors.lastname: Gao
authors.forename: Fang-Fang
authors.initials: FF
authors.lastname: Guan
authors.forename: Cheng-Yong
authors.initials: CY
authors.lastname: Li
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Liu
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Song
mesh_terms: D000669:Amplifiers, Electronic; D000906:Antibodies / Q000032:analysis* / Q000276:immunology; D000941:Antigens / Q000032:analysis* / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006735:Horseradish Peroxidase / Q000737:chemistry* / Q000276:immunology; D007118:Immunoassay / Q000295:instrumentation*; D015336:Molecular Probe Techniques / Q000295:instrumentation; D043942:Nanotubes / Q000737:chemistry / Q000648:ultrastructure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014025:Titanium / Q000737:chemistry*
chemical_list: D000906:Antibodies; D000941:Antigens; C009495:titanium dioxide; D014025:Titanium; D006735:Horseradish Peroxidase