title: The molecular organization of prenylated flavonoid xanthohumol in DPPC multibilayers: X-ray diffraction and FTIR spectroscopic studies.
abstract: Xanthohumol (XN) is the major prenylated flavonoid found in hop resin. It has attracted considerable attention in recent years due to its wide spectrum of biological activities and the beneficial effect on human health. Since lipid membrane is first target for biologically active compounds, we decided to investigate the influence of XN on the dipalmitoylphosphatidylcholine (DPPC) multibilayers. Interactions of XN with DPPC were investigated as a function of temperature and its concentration by using X-ray diffraction and the ATR-FTIR spectroscopy techniques. The aim of understanding the mechanisms of molecular interactions between XN and DPPC was to indicate the localization of the XN with respect to the membrane and the type of interaction with phospholipids. The results revealed that XN changes the physical properties of the DPPC multibilayers in the form of dry film. A new complex formation between XN and DPPC is reported. The detailed analysis of refraction effect indicates the changes in electron density ratio between hydrophobic and hydrophilic zones of lipid at phase transition. This is in compliance with reported changes in FTIR spectra where at pretransition XN moves from interface region between polar heads to the neighborhood of phosphate groups.
authors.affiliation: Department of Biophysics, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Arczewska
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Kamiński
authors.forename: Ewa
authors.initials: E
authors.lastname: Górecka
authors.forename: Damian
authors.initials: D
authors.lastname: Pociecha
authors.forename: Edward
authors.initials: E
authors.lastname: Rój
authors.forename: Adrianna
authors.initials: A
authors.lastname: Sławińska-Brych
authors.forename: Mariusz
authors.initials: M
authors.lastname: Gagoś
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D004583:Electrons; D005419:Flavonoids / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry; D008856:Microscopy, Fluorescence / Q000379:methods; D008956:Models, Chemical; D010710:Phosphates / Q000737:chemistry; D011427:Propiophenones / Q000737:chemistry*; D013053:Spectrophotometry / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013696:Temperature; D014961:X-Ray Diffraction / Q000379:methods*
chemical_list: D005419:Flavonoids; D008051:Lipid Bilayers; D008081:Liposomes; D010710:Phosphates; D011427:Propiophenones; D015060:1,2-Dipalmitoylphosphatidylcholine; C104536:xanthohumol