title: Hematocrit, viscosity and velocity distributions of aggregating and non-aggregating blood in a bifurcating microchannel.
abstract: Microscale blood flow is characterised by heterogeneous distributions of hematocrit, viscosity and velocity. In microvascular bifurcations, cells are unevenly distributed between the branches, and this effect can be amplified in subsequent branches depending on a number of parameters. We propose an approach to infer hematocrit profiles of human blood flowing through a bifurcating microchannel. The influence of aggregation, induced by the addition of Dextran 2000 to the samples, is also considered. Averaged values indicate plasma skimming, particularly in the presence of red blood cell (RBC) aggregation. Using an empirical model, the hematocrit profiles are used to estimate local relative viscosity distributions. Simulations are used to predict how the non-uniform viscosity influences the velocity profiles. Comparing these data to velocity profiles of RBCs measured using particle image velocimetry provides validation of the model. It is observed that aggregation blunts velocity profiles after a long straight section of channel. Downstream of the bifurcation, skewing of the velocity profiles is detected, which is enhanced by aggregation. The proposed methodology is capable of providing hitherto unreported information on important aspects of microscale blood rheology.
authors.affiliation: Department of Mechanical Engineering, University College London, London, UK.
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Sherwood
authors.forename: Efstathios
authors.initials: E
authors.lastname: Kaliviotis
authors.forename: Jonathan
authors.initials: J
authors.lastname: Dusting
authors.forename: Stavroula
authors.initials: S
authors.lastname: Balabani
mesh_terms: D001769:Blood; D006400:Hematocrit; D006801:Humans; D008954:Models, Biological; D014783:Viscosity