title: Proteome analysis of human cerebrospinal fluid as a diagnostic biomarker in patients with meningioma.
abstract: BACKGROUND
  To identify meningioma-specific proteins, cerebrospinal fluid (CSF) from 4 patients with a meningioma and 4 patients with a non-brain tumorous lesion were analyzed.
  
  
  METHODS
  Two-dimensional electrophoresis and electrospray quadrupole time-of-flight tandem mass spectrometry analyses revealed 10 unique spots, containing 11 independent proteins (spot #2 and #4 each contained 2 proteins and spot #3 was not identified) were evident in CSF associated with human meningioma: serum albumin precursor (3 different isoforms), Apolipoprotein E (Apo E), Apolipoprotein J precursor (Apo J), Transthyretin precursor (TTR), Prostaglandin D2 synthase 21 kDa (PTGDS), proapolipoprotein, Chain D hemoglobin Ypsilanti, alpha-1-antitrypsin (AAT), and beta-2-microglobulin precursor (β2M).
  
  
  RESULTS
  The contents of Apo E, Apo J and AAT were increased, while PTGDS, TTR and β2M were decreased.
  
  
  CONCLUSIONS
  The results observed by 2-dimensional electrophoresis were verified by Western blot analysis. The unique proteins may represent possible candidate biomarkers of meningioma.
authors.affiliation: Mass Spectrometry Research Center, Korea Basic Science Institute, Ochang, Republic of Korea.
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Sang Kwang
authors.initials: SK
authors.lastname: Lee
authors.forename: Yong Cheol
authors.initials: YC
authors.lastname: Yoo
authors.forename: Nam Hyun
authors.initials: NH
authors.lastname: Park
authors.forename: Dan Bi
authors.initials: DB
authors.lastname: Park
authors.forename: Jong Shin
authors.initials: JS
authors.lastname: Yoo
authors.forename: Hyun Joo
authors.initials: HJ
authors.lastname: An
authors.forename: Young Mok
authors.initials: YM
authors.lastname: Park
authors.forename: Kyung Gi
authors.initials: KG
authors.lastname: Cho
mesh_terms: D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D014408:Biomarkers, Tumor / Q000134:cerebrospinal fluid*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008577:Meningeal Neoplasms / Q000134:cerebrospinal fluid* / Q000175:diagnosis*; D008579:Meningioma / Q000134:cerebrospinal fluid* / Q000175:diagnosis*; D008875:Middle Aged; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000134:cerebrospinal fluid / Q000737:chemistry; D020543:Proteome / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D020543:Proteome