title: Mn(II) binding to human serum albumin: a ¹H-NMR relaxometric study.
abstract: Human serum albumin (HSA) displays several metal binding sites, participating to essential and toxic metal ions disposal and transport. The major Zn(II) binding site, called Site A, is located at the I/II domain interface, with residues His67, Asn99, His247, and Asp249 contributing with five donor atoms to the metal ion coordination. Additionally, one water molecule takes part of the octahedral coordination geometry. The occurrence of the metal-coordinated water molecule allows the investigation of the metal complex geometry by water (1)H-NMR relaxation, provided that the diamagnetic Zn(II) is replaced by the paramagnetic Mn(II). Here, the (1)H-NMR relaxometric study of Mn(II) binding to HSA is reported. Mn(II) binding to HSA is modulated by Zn(II), pH, and myristate through competitive inhibition and allosteric mechanisms. The body of results indicates that the primary binding site of Zn(II) corresponds to the secondary binding site of Mn(II), i.e. the multimetal binding site A. Excess Zn(II) completely displaces Mn(II) from its primary site suggesting that the primary Mn(II) site corresponds to the secondary Zn(II) site. This uncharacterized site is functionally-linked to FA1; moreover, metal ion binding is modulated by myristate and pH. Noteworthy, water (1)H-NMR relaxometry allowed a detailed analysis of thermodynamic properties of HSA-metal ion complexes.
authors.affiliation: Biomedical Research Division, Department of Theoretical and Applied Sciences, and Center of Neuroscience, University of Insubria, Via Alberto da Giussano 12, I-21052 Busto Arsizio (VA), Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Fanali
authors.forename: Yu
authors.initials: Y
authors.lastname: Cao
authors.forename: Paolo
authors.initials: P
authors.lastname: Ascenzi
authors.forename: Mauro
authors.initials: M
authors.lastname: Fasano
mesh_terms: D000495:Allosteric Site; D001665:Binding Sites; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008345:Manganese / Q000737:chemistry*; D019814:Myristic Acid / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D012709:Serum Albumin / Q000737:chemistry*; D013816:Thermodynamics; D014867:Water / Q000737:chemistry; D015032:Zinc / Q000737:chemistry
chemical_list: D012709:Serum Albumin; D014867:Water; D019814:Myristic Acid; D008345:Manganese; D015032:Zinc