title: Polymorphism (C677T) in the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene: A preliminary study on north Indian men.
abstract: An elevated level of plasma homocysteine, sulfur containing amino acid generated through demethylation of methionine has been widely accepted as a risk factor for cardiovascular disease (CVD). The increase can result from genetic and/or nutrient related disturbances in the remethylation or transsulfuration pathways for homocysteine metabolism. A common mutation (C677T) in the gene encoding for the enzyme 5, 10-methylenetetrahydrofolate reductase (MTHFR) or deficiency of the B vitamins namely folic acid, B(12), B(6) can lead to hyperhomocysteinemia.In the present study, we have investigated the incidence of the (C677T) MTHFR polymorphism in the North Indian males. 141 angiographically proven coronary artery disease (CAD) patients and 55 age and sex matched healthy volunteers were examined for the association between MTHFR gene polymorphism and CAD. The MTHFR genotyping was performed using polymerase chain reaction (PCR) followed by restriction-isotyping with Hinf 1 endonuclease. A trend for higher 'T' allele frequency (0.19) was observed in patients than in controls (0.16). However no significant association was found between C677T mutation and CAD severity. The lack of statistical significance could be due to the small sample size studied. Hence a larger study including various ethnic groups is warranted.
authors.affiliation: Department of Cardiology, Cardio-Thoracic & Neuro-Sciences Center, All India Institute of Medical Sciences, Ansari Nagar, 110029 New Delhi.
authors.forename: S
authors.initials: S
authors.lastname: Vasisht
authors.forename: R
authors.initials: R
authors.lastname: Gulati
authors.forename: R
authors.initials: R
authors.lastname: Narang
authors.forename: N
authors.initials: N
authors.lastname: Srivastava
authors.forename: L M
authors.initials: LM
authors.lastname: Srivastava
authors.forename: S C
authors.initials: SC
authors.lastname: Manchanda
authors.forename: D P
authors.initials: DP
authors.lastname: Agarwal
keywords: Genetic polymorphism; atherosclerosis; cardiovascular disease; hyperhomocysteinemia