title: A new candidate substrate for cell-matrix adhesion study: the acellular human amniotic matrix.
abstract: In vivo adhesions between cells and the extracellular matrix play a crucial role in cell differentiation, proliferation, and migration as well as tissue remodeling. Natural three-dimensional (3D) matrices, such as self-assembling matrices and Matrigel, have limitations in terms of their biomechanical properties. Here, we present a simple method to produce an acellular human amniotic matrix (AHAM) with preserved biomechanical properties and a favorable adhesion potential. On the stromal side of the AHAM, human foreskin fibroblasts (HFFs) attached and extended with bipolar spindle-shaped morphology proliferated to multilayer networks, invaded into the AHAM, and migrated in a straight line. Moreover, αV integrin, paxillin, and fibronectin were observed to colocalize after 24 h of HFF culture on the stromal side of the AHAM. Our results indicate that the AHAM may be an ideal candidate as a cell-matrix adhesion substrate to study cell adhesion and invasion as well as other functions in vitro under a tensile force that mimics the in vivo environment.
authors.affiliation: Department of Orthopaedics, General Hospital of Tianjin Medical University, 154 Anshan Road, Tianjin 300052, China.
authors.forename: Qianchen
authors.initials: Q
authors.lastname: Guo
authors.forename: Xuya
authors.initials: X
authors.lastname: Lu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xue
authors.forename: Hong
authors.initials: H
authors.lastname: Zheng
authors.forename: Xiaotao
authors.initials: X
authors.lastname: Zhao
authors.forename: Huajian
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000268:Adhesiveness; D000650:Amnion / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology*; D002474:Cell-Free System / Q000502:physiology; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000502:physiology*; D005347:Fibroblasts / Q000502:physiology*; D022001:Focal Adhesions / Q000502:physiology*; D006801:Humans