title: Characterization of Erysiphe necator-responsive genes in Chinese Wild Vitis quinquangularis.
abstract: Powdery mildew (PM), caused by fungus Erysiphe necator, is one of the most devastating diseases of grapevine. To better understand grapevine-PM interaction and provide candidate resources for grapevine breeding, a suppression subtractive hybridization (SSH) cDNA library was constructed from E. necator-infected leaves of a resistant Chinese wild Vitis quinquangularis clone "Shang-24". A total of 492 high quality expressed sequence tags (ESTs) were obtained and assembled into 266 unigenes. Gene ontology (GO) analysis indicated that 188 unigenes could be assigned with at least one GO term in the biological process category, and 176 in the molecular function category. Sequence analysis showed that a large number of these genes were homologous to those involved in defense responses. Genes involved in metabolism, photosynthesis, transport and signal transduction were also enriched in the library. Expression analysis of 13 selected genes by qRT-PCR revealed that most were induced more quickly and intensely in the resistant material "Shang-24" than in the sensitive V. pseudoreticulata clone "Hunan-1" by E. necator infection. The ESTs reported here provide new clues to understand the disease-resistance mechanism in Chinese wild grapevine species and may enable us to investigate E. necator-responsive genes involved in PM resistance in grapevine germplasm.
authors.affiliation: College of Horticulture, Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China.|State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling, Shaanxi 712100, China.
authors.forename: Min
authors.initials: M
authors.lastname: Gao
authors.affiliation: College of Horticulture, Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China.|State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling, Shaanxi 712100, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Niu
authors.affiliation: College of Horticulture, Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China.|State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling, Shaanxi 712100, China.
authors.forename: Suping
authors.initials: S
authors.lastname: Zhao
authors.affiliation: College of Horticulture, Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China.|State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling, Shaanxi 712100, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Jiao
authors.affiliation: School of Agriculture, Ningxia University, Yinchuan, Ningxia 750021, China.
authors.forename: Weirong
authors.initials: W
authors.lastname: Xu
authors.affiliation: Boyce Thompson Institute, Cornell University, Ithaca, NY 14853, USA.|USDA Robert W. Holley Center for Agriculture and Health, Ithaca, NY 14853, USA.
authors.forename: Zhangjun
authors.initials: Z
authors.lastname: Fei
authors.affiliation: College of Horticulture, Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China.|State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling, Shaanxi 712100, China.
authors.forename: Xiping
authors.initials: X
authors.lastname: Wang
mesh_terms: D001203:Ascomycota / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D027843:Vitis / Q000276:immunology / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal
keywords: Chinese wild Vitis quinquangularis; EST; Erysiphe necator; SSH; qRT-PCR