title: Comparison of different methods for determining beta-lactam susceptibility in Pseudomonas aeruginosa.
abstract: This study compared the results of antimicrobial susceptibility testing of 77 clinical strains isolated for Pseudomonas aeruginosa to five beta-lactam agents: aztreonam, ceftazidime, imipenem, meropenem and piperacillin+tazobactam. Four different methods were employed: two automated systems (VITEK 2 and Sensititre) and two standardized manual methods (Kirby-Bauer and E-test). The concordances for the susceptibility categories were better for Kirby-Bauer (medium value =89.6%), followed by Sensititre (medium value =87.0%) and VITEK 2 (medium value =82.8%). The disk diffusion method did not present very major errors in comparison to the two automated systems.
authors.affiliation: Department of Clinical Pathology, Ospedale dell'Angelo, Mestre, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Sapino
authors.forename: Sandra
authors.initials: S
authors.lastname: Mazzuccato
authors.forename: Maria
authors.initials: M
authors.lastname: Solinas
authors.forename: Massimo
authors.initials: M
authors.lastname: Gion
authors.forename: Stefano
authors.initials: S
authors.lastname: Grandesso
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D011552:Pseudomonas Infections; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D047090:beta-Lactams / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams