title: Effect of pioglitazone on the fructose-induced abdominal adipose tissue dysfunction.
abstract: Aim. To test the potential role of PPARγ in the endocrine abdominal tissue dysfunction induced by feeding normal rats with a fructose rich diet (FRD) during three weeks. Methodology. Adult normal male rats received a standard commercial diet (CD) or FRD, (10% in drinking water) without or with pioglitazone (PIO) (i.p. 0.25 mg/Kg BW/day; CD-PIO and FRD-PIO). Thereafter, we measured circulating metabolic, endocrine, and oxidative stress (OS) markers, abdominal adipose tissue (AAT) mass, leptin (LEP) and plasminogen activator inhibitor-1 (PAI-1) tissue content/expression, and leptin release by isolated adipocytes incubated with different concentrations of insulin. Results. Plasma glucose, insulin, triglyceride, TBARS, LEP, and PAI-1 levels were higher in FRD rats; PIO coadministration fully prevented all these increments. AAT adipocytes from FRD rats were larger, secreted a higher amount of LEP, and displayed decreased sensitivity to insulin stimulation; these effects were significantly ameliorated by PIO. Whereas AAT LEP and PAI-1 (mRNA) concentrations increased significantly in FRD rats, those of insulin-receptor-substrate- (IRS-) 1 and IRS-2 were reduced. PIO coadministration prevented FRD effects on LEP, PAI-1, and IRS-2 (fully) and IRS-1 (partially) mRNAs in AAT. Conclusion. PPARγ would play a relevant role in the development of the FRD-induced metabolic-endocrine dysfunction.
authors.affiliation: Neuroendocrine Unit IMBICE, CICPBA, and CONICET, P.O. Box 403, 1900 La Plata, Argentina.
authors.forename: Ana
authors.initials: A
authors.lastname: Alzamendi
authors.forename: Andrés
authors.initials: A
authors.lastname: Giovambattista
authors.forename: María E
authors.initials: ME
authors.lastname: García
authors.forename: Oscar R
authors.initials: OR
authors.lastname: Rebolledo
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Gagliardino
authors.forename: Eduardo
authors.initials: E
authors.lastname: Spinedi