title: Sensitivity of translation initiation factor eIF1 as a molecular target of salt toxicity to sodic-alkaline stress in the halophytic grass Leymus chinensis.
abstract: Eukaryotic translation initiation factors (eIFs) have been shown to be critical in the initiation of protein synthesis. Here, we report the cloning and characterization of a novel gene, LceIF1, from a potentially interesting forage grass, Leymus chinensis (Trin.). The expression results show that LceIF1 is expressed in most organisms under normal conditions, but the transcription patterns differ under sodic-saline and sodic-alkaline stresses. Sodic-saline stress induced a persistent decrease, and sodic-alkaline stress induced overexpression of LceIF1. Potassic-saline and alkaline stresses did not cause any changes in expression of eIF1. These results indicate that not only pH but also Na(+) concentration affects overtranscription of LceIF1. The eIF1 transgenic lines showed relatively high eIF1 expression, resulting in potentially higher stress resistance. Combined with eIF1 transcription in transgenic lines, LceIF1 as a molecular target of salt toxicity is believed to help enhance salt tolerance.
authors.affiliation: School of Life Sciences, Ludong University, Yantai, 264-025, Shandong, China. ylsun04@mails.ucas.ac.cn
authors.forename: Yan-Lin
authors.initials: YL
authors.lastname: Sun
authors.forename: Soon-Kwan
authors.initials: SK
authors.lastname: Hong
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D039642:Eukaryotic Initiation Factors / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D006109:Poaceae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000633:toxicity*; D013312:Stress, Physiological
chemical_list: D017931:DNA Primers; D039642:Eukaryotic Initiation Factors; D012965:Sodium Chloride