title: Dissociation of membrane-anchored heterotrimeric G-protein induced by G(α) subunit binding to GTP.
abstract: Heterotrimeric G-proteins' activation on the intracellular side of the cell membrane is initiated by stimulation of the G-Protein Coupled Receptors (GPCRs) extra-cellular part. This two-step activation mechanism includes (1) an exchange between GDP and GTP molecules in the G(α) subunit and (2) a dissociation of the whole G(αβγ) complex into two membrane-anchored blocks, namely the isolated G(α) and G(βγ) subunits. Although X-ray data are available for both inactive G(αβγ):GDP and active G(α):GTP complexes, intermediate steps involved in the molecular mechanism of the dissociation have not yet been addressed at the molecular level. In this study, we first built a membrane-anchored intermediate G(iαβγ):GTP complex. This model was then equilibrated by molecular dynamics simulations before the Targeted Molecular Dynamics (TMD) technique was used to force the G(α) subunit to evolve from its inactive (GDP-bound) to its active (GTP-bound) conformations, as described by available X-ray data. The TMD constraint was applied only to the G(α) subunit so that the resulting global rearrangements acting on the whole G(αβγ):GTP heterotrimer could be analyzed. We showed how these mainly local conformational changes of G(α) could initiate large domain:domain motions of the whole complex, the G(βγ) behaving as an almost quasi-rigid block. This separation of the two G(α):GTP and G(βγ) subunits required the loss of several interactions at the G(α):G(βγ) interface that were reported. This study provided an atomistic view of the crucial intermediate step of the G-proteins activation, e.g., the dissociation, that could hardly be elucidated by the experiment.
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), CNRS UMR5247, Université Montpellier 1, Université Montpellier 2, Faculté de Pharmacie, 15 av. Charles Flahault, BP 14491, 34093 Montpellier Cedex 05, France.
authors.forename: Maxime
authors.initials: M
authors.lastname: Louet
authors.forename: Landry
authors.initials: L
authors.lastname: Charlier
authors.forename: Jean
authors.initials: J
authors.lastname: Martinez
authors.forename: Nicolas
authors.initials: N
authors.lastname: Floquet
mesh_terms: D018360:Crystallography, X-Ray; D006153:Guanosine Diphosphate / Q000737:chemistry*; D006160:Guanosine Triphosphate / Q000737:chemistry*; D020962:Heterotrimeric GTP-Binding Proteins / Q000737:chemistry*; D007700:Kinetics; D056004:Molecular Dynamics Simulation; D010713:Phosphatidylcholines / Q000737:chemistry*; D011485:Protein Binding; D021122:Protein Subunits / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D010713:Phosphatidylcholines; D021122:Protein Subunits; D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate; D020962:Heterotrimeric GTP-Binding Proteins; C028694:1-palmitoyl-2-oleoylphosphatidylcholine