title: A novel nuclear protein phosphatase 2C negatively regulated by ABL1 is involved in abiotic stress and panicle development in rice.
abstract: Type 2C protein phosphatase plays an important role in the signal transduction of stress response in plants. In this paper, we identified a novel stress-induced type 2C protein phosphatase gene OsSIPP2C1 from rice. OsSIPP2C1 contains a complete open reading frame of 1,074 bp, encoding a protein with 357 amino acids. OsSIPP2C1 expression was up-regulated by high salt, PEG6000 and exogenous ABA, and enhanced in the abl1 mutant under normal, salt, or drought condition. Interestingly, OsSIPP2C1 expression was increased during the early panicle development. Subcellular localization assay using rice protoplast cells indicated that OsSIPP2C1 was predominantly located in the nucleus. Together, it is suggested that a nuclear PP2C protein OsSIPP2C1 negatively regulated by ABL1 is involved in abiotic stress and panicle development in rice.
authors.affiliation: Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
authors.forename: Yu-Sheng
authors.initials: YS
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.forename: Zhou-Fei
authors.initials: ZF
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Duan
authors.forename: Sheng-Dong
authors.initials: SD
authors.lastname: Huang
authors.forename: Juan
authors.initials: J
authors.lastname: Yang
authors.forename: Ji
authors.initials: J
authors.lastname: Huang
authors.forename: Hong-Sheng
authors.initials: HS
authors.lastname: Zhang
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D015870:Gene Expression; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D016366:Open Reading Frames; D012275:Oryza / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D000071636:Protein Phosphatase 2C; D011523:Protoplasts / Q000378:metabolism; D016415:Sequence Alignment; D013312:Stress, Physiological / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D009687:Nuclear Proteins; D010940:Plant Proteins; D014157:Transcription Factors; D000040:Abscisic Acid; D010749:Phosphoprotein Phosphatases; D000071636:Protein Phosphatase 2C