title: Biosynthesis of nicotinic acid from 3-cyanopyridine by a newly isolated Fusarium proliferatum ZJB-09150.
abstract: In this study, nitriles were used as sole sources of nitrogen in the enrichments to isolate nitrile-converting microorganisms. A novel fungus named ZJB-09150 possessing nitrile-converting enzymes was obtained with 3-cyanopyridine as sole source of nitrogen, which was identified by morphology, biology and 18S rDNA gene sequence as Fusarium proliferatum. It was found that F. proliferatum had ability to convert nitriles to corresponding acids or amides and showed wide substrate specificity to aliphatic nitriles, aromatic nitriles and ortho-substituted heterocyclic nitriles. The nitrile converting enzymes including nitrilase and nitrile hydratase in ZJB-09150 were induced by ε-caprolactam. Nitrilase obtained in this study showed high activity toward 3-cyanopyridine. It was active within pH 3.0-12.0 and temperature ranging from 25 to 65 °C with optimal at pH 9.0 and temperature 50-55 °C. The enzyme was thermostable and its half-life was 12.5 and 6 h at 45 and 55 °C, respectively. Under optimized reaction conditions, 60 mM 3-cyanopyridine was converted to nicotinic acid in 15 min, which indicated ZJB-09150 has potentials of application in large scale production of nicotinic acid.
authors.affiliation: Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China.
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Jin
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Liu
authors.forename: Jian-Miao
authors.initials: JM
authors.lastname: Xu
authors.forename: Yu-Guo
authors.initials: YG
authors.lastname: Zheng
mesh_terms: D000619:Aminohydrolases / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods; D005670:Fusarium / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D006836:Hydro-Lyases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009539:Nicotinic Acids / Q000096:biosynthesis*; D009570:Nitriles / Q000378:metabolism; D011725:Pyridines / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D009539:Nicotinic Acids; D009570:Nitriles; D011725:Pyridines; D000619:Aminohydrolases; C021867:nitrilase; D006836:Hydro-Lyases; C048831:nitrile hydratase; C020729:3-cyanopyridine