title: Characterisation of four major histocompatibility complex class II genes of the koala (Phascolarctos cinereus).
abstract: Major histocompatibility complex (MHC) class II molecules have an integral role in the adaptive immune response, as they bind and present antigenic peptides to T helper lymphocytes. In this study of koalas, species-specific primers were designed to amplify exon 2 of the MHC class II DA and DB genes, which contain much of the peptide-binding regions of the α and β chains. A total of two DA α1 domain variants and eight DA β1 (DAB), three DB α1 and five DB β1 variants were amplified from 20 koalas from two free-living populations from South East Queensland and the Port Macquarie region in northern New South Wales. We detected greater variation in the β1 than in the α1 domains as well as evidence of positive selection in DAB. The present study provides a springboard to future investigation of the role of MHC in disease susceptibility in koalas.
authors.affiliation: Faculty of Veterinary Science, The University of Sydney, NSW 2006, Australia.
authors.forename: Quintin
authors.initials: Q
authors.lastname: Lau
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Jobbins
authors.forename: Katherine
authors.initials: K
authors.lastname: Belov
authors.forename: Damien P
authors.initials: DP
authors.lastname: Higgins
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D023521:Chlamydophila Infections / Q000276:immunology / Q000662:veterinary; D017931:DNA Primers; D005784:Gene Amplification; D005802:Genes, MHC Class II; D014644:Genetic Variation; D005838:Genotype; D000949:Histocompatibility Antigens Class II / Q000737:chemistry; D008969:Molecular Sequence Data; D048368:Phascolarctidae / Q000235:genetics* / Q000276:immunology*; D010802:Phylogeny; D016415:Sequence Alignment; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
chemical_list: D017931:DNA Primers; D000949:Histocompatibility Antigens Class II