title: Characterization of an attenuated TE3L-deficient vaccinia virus Tian Tan strain.
abstract: An attenuated vaccinia virus (VACV), TE3L(-)VTT, was evaluated for virulence and safety to determine its potential use as a vaccine or as a recombinant virus vector to express foreign genes. The virulence of TE3L(-)VTT was compared with that of the wild-type VTT both in vivo and in vitro. The humoral and cellular immune responses were detected in a mouse model to test the vaccine efficacy of the TE3L mutant. The results suggested that deletion of the TE3L gene decreased the virulence and neurovirulence significantly in mice and rabbit models, yet retained the immunogenicity. Thus, the deletion of TE3L improved the safety of the VTT vector; this approach may yield a valuable resource for studies of recombinant VACV-vectored vaccines.
authors.affiliation: Institute of Military Veterinary Medicine, Academy of Military Medical Sciences of PLA, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun 130122, PR China.
authors.forename: Yuhang
authors.initials: Y
authors.lastname: Wang
authors.forename: Shifu
authors.initials: S
authors.lastname: Kan
authors.forename: Shouwen
authors.initials: S
authors.lastname: Du
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Qi
authors.forename: Jinhui
authors.initials: J
authors.lastname: Wang
authors.forename: Liming
authors.initials: L
authors.lastname: Liu
authors.forename: Huifan
authors.initials: H
authors.lastname: Ji
authors.forename: Dongyun
authors.initials: D
authors.lastname: He
authors.forename: Na
authors.initials: N
authors.lastname: Wu
authors.forename: Chang
authors.initials: C
authors.lastname: Li
authors.forename: Baorong
authors.initials: B
authors.lastname: Chi
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.forename: Ningyi
authors.initials: N
authors.lastname: Jin
mesh_terms: D000818:Animals; D002470:Cell Survival; D002522:Chlorocebus aethiops; D005260:Female; D017353:Gene Deletion; D005814:Genes, Viral; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology; D006367:HeLa Cells; D006801:Humans; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007928:Lethal Dose 50; D061985:Madin Darby Canine Kidney Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D012867:Skin / Q000473:pathology / Q000821:virology; D012899:Smallpox / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D014611:Vaccination / Q000379:methods; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D014709:Vero Cells; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*; D014774:Virulence; D014779:Virus Replication
chemical_list: D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic; D014765:Viral Vaccines