title: Imaging of lysosomal pH changes with a fluorescent sensor containing a novel lysosome-locating group.
abstract: Rlyso, a highly selective and sensitive pH sensor, can stain lysosomes with a novel lysosome-locating group, methylcarbitol. Rlyso was successfully used to detect lysosomal pH changes during apoptosis or induced by chloroquine while avoiding the "alkalizing effect" on lysosomes of current lysosomal probes with nitrogen-containing sidechains.
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, High-tech District, Dalian 116024, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhu
authors.forename: Jiangli
authors.initials: J
authors.lastname: Fan
authors.forename: Qunli
authors.initials: Q
authors.lastname: Xu
authors.forename: Honglin
authors.initials: H
authors.lastname: Li
authors.forename: Jingyun
authors.initials: J
authors.lastname: Wang
authors.forename: Pan
authors.initials: P
authors.lastname: Gao
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Peng
mesh_terms: D000818:Animals; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002738:Chloroquine / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D005026:Ethylene Glycols / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008247:Lysosomes / Q000737:chemistry*; D051379:Mice; D008856:Microscopy, Fluorescence; D013050:Spectrometry, Fluorescence
chemical_list: D018931:Antineoplastic Agents, Hormonal; D005026:Ethylene Glycols; D005456:Fluorescent Dyes; C048214:diethylene glycol monomethyl ether; D003907:Dexamethasone; D002738:Chloroquine