title: Construction of short tandem target mimic (STTM) to block the functions of plant and animal microRNAs.
abstract: Small RNAs are widespread in plants and animals. They largely include microRNAs (miRNAs) and short interfering RNAs (siRNAs), and they play key roles in gene and chromatin regulations. Here we describe in detail the method for an effective construction of the recently developed short tandem target mimic (STTM) technology to block small RNA functions in plants and animals. STTM is a powerful technology complementing the previous target mimic (TM) in plants and the miRNA sponge, as well as the recently defined endogenous competing RNA (CeRNA) in animals. We expect STTM will not only be effective in blocking small RNA functions in plants but will also become a popular approach in animals.
authors.affiliation: Department of Biological Sciences, Michigan Technological University, Houghton, MI 49931, USA. gtang1@mtu.edu
authors.forename: Guiliang
authors.initials: G
authors.lastname: Tang
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.forename: Yiyou
authors.initials: Y
authors.lastname: Gu
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Qiao
authors.forename: Ruiwen
authors.initials: R
authors.lastname: Fan
authors.forename: Yiping
authors.initials: Y
authors.lastname: Mao
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Tang
mesh_terms: D000818:Animals; D018506:Gene Expression Regulation, Plant; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics; D010944:Plants / Q000235:genetics; D034741:RNA, Small Interfering / Q000737:chemistry; D017423:Sequence Analysis, RNA
chemical_list: C546086:MIRN30b microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering