title: Changes in the BAG1 expression of Schwann cells after sciatic nerve crush.
abstract: Bcl-2-associated athanogene-1 (BAG1), a co-chaperone for Hsp70/Hsc70, is a multifunctional protein, which has been shown to suppress apoptosis and enhance neuronal differentiation. However, the expression and roles of BAG1 in peripheral system lesions and repair are still unknown. In this study, we investigated the dynamic changes in BAG1 expression in an acute sciatic nerve crush model in adult rats. Western blot analysis revealed that BAG1 was expressed in normal sciatic nerves. BAG1 expression increased progressively after sciatic nerve crush, reached a peak 2 weeks post-injury, and then returned to the normal level 4 weeks post-injury. Spatially, we observed that BAG1 was mainly expressed in Schwann cells and that BAG1 expression increased in Schwann cells after injury. In vitro, we found that BAG1 expression increased during the cyclic adenosine monophosphate (cAMP)-induced Schwann cell differentiation process. BAG1-specific siRNA inhibited cAMP-induced Schwann cell differentiation. In conclusion, we speculated that BAG1 was upregulated in the sciatic nerve after crush, which was associated with Schwann cell differentiation.
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Liu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Long
authors.initials: L
authors.lastname: Long
authors.forename: Xinghai
authors.initials: X
authors.lastname: Cheng
authors.forename: Lei
authors.initials: L
authors.lastname: Ji
authors.forename: Hai
authors.initials: H
authors.lastname: Weng
authors.forename: Tao
authors.initials: T
authors.lastname: Ding
authors.forename: Jiao
authors.initials: J
authors.lastname: Yang
authors.forename: Haixiang
authors.initials: H
authors.lastname: Wei
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Weipeng
authors.initials: W
authors.lastname: Huan
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Deng
authors.forename: Youhua
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D005786:Gene Expression Regulation; D008297:Male; D009409:Nerve Crush; D009416:Nerve Regeneration / Q000235:genetics / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D050816:Octamer Transcription Factor-6 / Q000096:biosynthesis / Q000235:genetics; D061251:Primary Cell Culture; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000378:metabolism* / Q000473:pathology; D012584:Sciatic Nerve / Q000293:injuries* / Q000502:physiology; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*
chemical_list: C091732:BCL2-associated athanogene 1 protein; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; D034741:RNA, Small Interfering; D014157:Transcription Factors; D050816:Octamer Transcription Factor-6; D000242:Cyclic AMP