title: μ-PADs for detection of chemical warfare agents.
abstract: Conventional methods of detection of chemical warfare agents (CWAs) based on chromogenic reactions are time and solvent intensive. The development of cost, time and solvent effective microfluidic paper based analytical devices (μ-PADs) for the detection of nerve and vesicant agents is described. The detection of analytes was based upon their reactions with rhodamine hydroxamate and para-nitrobenzyl pyridine, producing red and blue colours respectively. Reactions were optimized on the μ-PADs to produce the limits of detection (LODs) as low as 100 μM for sulfur mustard in aqueous samples. Results were quantified with the help of a simple desktop scanner and Photoshop software. Sarin achieved a linear response in the two concentration ranges of 20-100 mM and 100-500 mM, whereas the response of sulfur mustard was found to be linear in the concentration range of 10-75 mM. Results were precise enough to establish the μ-PADs as a valuable tool for security personnel fighting against chemical terrorism.
authors.affiliation: Vertox Laboratory, Defence Research and Development Establishment, Jhansi Road, Gwalior 474002, India.
authors.forename: Deepak
authors.initials: D
authors.lastname: Pardasani
authors.forename: Vijay
authors.initials: V
authors.lastname: Tak
authors.forename: Ajay K
authors.initials: AK
authors.lastname: Purohit
authors.forename: D K
authors.initials: DK
authors.lastname: Dubey
mesh_terms: D002619:Chemical Warfare Agents / Q000032:analysis*; D002863:Chromogenic Compounds; D057230:Limit of Detection; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009151:Mustard Gas / Q000032:analysis; D010209:Paper; D011725:Pyridines / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry*; D012524:Sarin / Q000032:analysis
chemical_list: D002619:Chemical Warfare Agents; D002863:Chromogenic Compounds; D011725:Pyridines; D012235:Rhodamines; C008633:4-(4-nitrobenzyl)pyridine; D012524:Sarin; D009151:Mustard Gas