title: FTIR, (1)H NMR and EPR spectroscopy studies on the interaction of flavone apigenin with dipalmitoylphosphatidylcholine liposomes.
abstract: Apigenin (5,7,4'-trihydroxyflavone) is a cancer chemopreventive agent and a member of the family of plant flavonoids. Apigenin interaction with liposomes formed with dipalmitoylphosphatidylcholine (DPPC) was investigated by means of FTIR spectroscopy, (1)H NMR and EPR techniques. Fluorescent microscopy and electron microscopy were applied to study the apigenin effects on colon myofibroblasts and human skin fibroblasts. The strong rigidifying effect of apigenin with respect to polar head groups was concluded on the basis of the action of the flavone on partition coefficient of Tempo spin label between the water and lipid phases. The ordering effect was also found in hydrophobic region at the depth monitored by 5-SASL and 16-SASL spin labels. The inclusion of apigenin to the membrane restricted the motional freedom of polar head groups lowering penetration of Pr(3+) ions to the membranes. The (1)H NMR technique supported also the restriction of motional freedom of the membrane in the hydrophobic region, especially in the zone of CH(2) groups of alkyl chains. FTIR analysis showed that apigenin incorporates into DPPC liposomes via hydrogen bonding between its own hydroxyl groups and lipid polar head groups in the C-O-P-O-C segment. It is also very likely that hydroxyl groups of apigenin link with polar groups of DPPC by water bridges. Electron and fluorescence microscopic observations revealed changes in the internal membrane organization of the examined cells. In conclusion, the changes of the structural and dynamic properties of membranes can be crucial for processes involving tumor suppression signal transduction pathways and cell cycle regulation.
authors.affiliation: Department of Comparative Anatomy and Anthropology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland. bozka1996@o2.pl
authors.forename: Bożena
authors.initials: B
authors.lastname: Pawlikowska-Pawlęga
authors.forename: Lucjan E
authors.initials: LE
authors.lastname: Misiak
authors.forename: Barbara
authors.initials: B
authors.lastname: Zarzyka
authors.forename: Roman
authors.initials: R
authors.lastname: Paduch
authors.forename: Antoni
authors.initials: A
authors.lastname: Gawron
authors.forename: Wiesław I
authors.initials: WI
authors.lastname: Gruszecki
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D047310:Apigenin / Q000737:chemistry* / Q000494:pharmacology; D003106:Colon / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D005347:Fibroblasts / Q000378:metabolism; D047309:Flavones / Q000737:chemistry*; D006801:Humans; D007477:Ions; D008081:Liposomes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D008956:Models, Chemical; D015398:Signal Transduction; D012867:Skin / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013113:Spin Labels; D014867:Water / Q000737:chemistry
chemical_list: D047309:Flavones; D007477:Ions; D008081:Liposomes; D013113:Spin Labels; D014867:Water; D015060:1,2-Dipalmitoylphosphatidylcholine; D047310:Apigenin