title: Review on recent advances in the analysis of isolated organelles.
abstract: The analysis of isolated organelles is one of the pillars of modern bioanalytical chemistry. This review describes recent developments on the isolation and characterization of isolated organelles both from living organisms and cell cultures. Salient reports on methods to release organelles focused on reproducibility and yield, membrane isolation, and integrated devices for organelle release. New developments on organelle fractionation after their isolation were on the topics of centrifugation, immunocapture, free flow electrophoresis, flow field-flow fractionation, fluorescence activated organelle sorting, laser capture microdissection, and dielectrophoresis. New concepts on characterization of isolated organelles included atomic force microscopy, optical tweezers combined with Raman spectroscopy, organelle sensors, flow cytometry, capillary electrophoresis, and microfluidic devices.
authors.affiliation: Department of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA.
authors.forename: Chad P
authors.initials: CP
authors.lastname: Satori
authors.forename: Vratislav
authors.initials: V
authors.lastname: Kostal
authors.forename: Edgar A
authors.initials: EA
authors.lastname: Arriaga
mesh_terms: D000818:Animals; D002458:Cell Fractionation; D019075:Electrophoresis, Capillary; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007163:Immunosorbent Techniques; D046210:Microfluidic Analytical Techniques; D018625:Microscopy, Atomic Force; D052898:Optical Tweezers; D015388:Organelles / Q000737:chemistry* / Q000378:metabolism; D013059:Spectrum Analysis, Raman
chemical_list: D005456:Fluorescent Dyes