title: [A new construct of dna reporter for immuno-PCR].
abstract: A new construct of DNA reporter has been designed for protein quantification by immuno-PCR. It has been shown that amplification efficiency of a reporter that contains a fragment of human adenovirus 2 flanked by homoprimer sequences is much higher vs. standard PCR format based on use of two different primers. Application of a new construct and its homoprimer-based detection opens a way to a significant increase in the immuno-PCR sensitivity and the efficiency of single molecule PCR.
authors.forename: I G
authors.initials: IG
authors.lastname: Nikitina
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Bukurova
authors.forename: V L
authors.initials: VL
authors.lastname: Karpov
authors.forename: N A
authors.initials: NA
authors.lastname: Lisitsyn
authors.forename: S F
authors.initials: SF
authors.lastname: Beresten'
mesh_terms: D000256:Adenoviridae; D000818:Animals; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D004279:DNA, Viral