title: The inhibitory effect of Prunella vulgaris L. on aldose reductase and protein glycation.
abstract: To evaluate the aldose reductase (AR) enzyme inhibitory ability of Prunella vulgaris L. extract, six compounds were isolated and tested for their effects. The components were subjected to in vitro bioassays to investigate their inhibitory assays using rat lens aldose reductase (rAR) and human recombinant AR (rhAR). Among them, caffeic acid ethylene ester showed the potent inhibition, with the IC(50) values of rAR and rhAR at 3.2 ± 0.55 μM and 12.58 ± 0.32 μM, respectively. In the kinetic analyses using Lineweaver-Burk plots of 1/velocity and 1/concentration of substrate, this compound showed noncompetitive inhibition against rhAR. Furthermore, it inhibited galactitol formation in a rat lens incubated with a high concentration of galactose. Also it has antioxidative as well as advanced glycation end products (AGEs) inhibitory effects. As a result, this compound could be offered as a leading compound for further study as a new natural products drug for diabetic complications.
authors.affiliation: Institute of Natural Medicine, Hallym University, Chuncheon 200-702, Republic of Korea.
authors.forename: Hong Mei
authors.initials: HM
authors.lastname: Li
authors.forename: Jin Kyu
authors.initials: JK
authors.lastname: Kim
authors.forename: Jai Man
authors.initials: JM
authors.lastname: Jang
authors.forename: Sang Oh
authors.initials: SO
authors.lastname: Kwon
authors.forename: Cheng Bi
authors.initials: CB
authors.lastname: Cui
authors.forename: Soon Sung
authors.initials: SS
authors.lastname: Lim
mesh_terms: D062107:Advanced Oxidation Protein Products / Q000737:chemistry*; D000449:Aldehyde Reductase / Q000737:chemistry*; D000818:Animals; D004791:Enzyme Inhibitors / Q000737:chemistry; D017127:Glycation End Products, Advanced / Q000737:chemistry*; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry*; D031347:Prunella / Q000737:chemistry*; D051381:Rats
chemical_list: D062107:Advanced Oxidation Protein Products; D004791:Enzyme Inhibitors; D017127:Glycation End Products, Advanced; D010936:Plant Extracts; D000449:Aldehyde Reductase