title: Comparison of MagNA Pure 96, Chemagic MSM1, and QIAamp MinElute for hepatitis B virus nucleic acid extraction.
abstract: This study was designed to compare two automated systems and one manual system for hepatitis B virus (HBV) nucleic acid extraction. The two automated systems were the MagNA Pure 96 system (Roche Applied Science, Manheim, Germany) and the Chemagic system (Chemagen, Baesweiler, Germany), and the manual system was the QIAamp system (Qiagen, Hilden, Germany). Sixty-eight samples that were within the detection range of the Cobas Ampliprep/Cobas TaqMan (CAP/CTM) platform (Roche Molecular Systems, Manheim, Germany) were selected. Extracted viral nucleic acids from the three systems were quantified using an AccuPower HBV Quantitative PCR kit (Bioneer, Daejon, Korea). The MagNA Pure 96 system and QIAamp system did not detect viral loads in one sample. The Chemagic system did not detect low viral loads in nine samples (range, 26-290 IU/mL by the CAP/CTM platform). Comparisons of the viral loads of the samples from the MagNA Pure 96 system, the Chemagic system, and the QIAamp system with those from the CAP/CTM platform yielded correlation coefficients of 0.977, 0.914, and 0.967, respectively. Comparisons of the MagNA Pure 96 system and the Chemagic system with the QIAamp system yielded correlation coefficients of 0.987 and 0.939, respectively. The MagNA Pure 96 system demonstrated better performance than the Chemagic system for HBV nucleic acid extraction. The MagNA Pure 96 system demonstrated comparable performance with the QIAamp system.
authors.affiliation: Department of Laboratory Medicine, Chosun University Medical School, Gwang-Ju, South Korea. seonghomed@hanmail.net
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Kang
authors.forename: Eun Hee
authors.initials: EH
authors.lastname: Lee
authors.forename: Geon
authors.initials: G
authors.lastname: Park
authors.forename: Sook Jin
authors.initials: SJ
authors.lastname: Jang
authors.forename: Dae Soo
authors.initials: DS
authors.lastname: Moon
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D006515:Hepatitis B virus / Q000737:chemistry*; D009696:Nucleic Acids / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction; D019562:Viral Load / Q000295:instrumentation* / Q000379:methods*
chemical_list: D009696:Nucleic Acids