title: Co-regulation of polar mRNA transport and lifespan in budding yeast Saccharomyces cerevisiae.
abstract: Recent studies have uncovered the links between aging, rejuvenation and polar protein transport in the budding yeast Saccharomyces cerevisiae. Here, we examined a still unexplored possibility for co-regulation of polar mRNA transport and lifespan. To monitor the amount and distribution of mRNA-containing granules in mother and daughter cells, we used a fluorescent mRNA-labeling system, with MFA2 as a reporter gene. The results obtained showed that deletion of the selected longevity regulators in budding yeast had a significant impact on the polar mRNA transport. This included changes in the amount of mRNA-containing granules in cytoplasm, their aggregation and distribution between the mother and daughter cells. A significant negative correlation was found between strain-specific longevity, amount of granules and total fluorescent intensity both in mother and daughter cells. As indicated by the coefficient of determination, approximately 50-75% of variation in yeast lifespan could be attributed to the differences in polar mRNA transport.
authors.affiliation: The Shraga Segal Department of Microbiology, Immunology and Genetics, Center for Multidisciplinary Research on Aging, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
authors.forename: Dmitri
authors.initials: D
authors.lastname: Taranukha
authors.forename: Arie
authors.initials: A
authors.lastname: Budovsky
authors.forename: Nikolai
authors.initials: N
authors.lastname: Gobshtis
authors.forename: Alex
authors.initials: A
authors.lastname: Braiman
authors.forename: Ziv
authors.initials: Z
authors.lastname: Porat
authors.forename: Stella
authors.initials: S
authors.lastname: Aronov
authors.forename: Vadim E
authors.initials: VE
authors.lastname: Fraifeld
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry; D017930:Genes, Reporter; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism; D008136:Longevity; D018613:Microscopy, Confocal; D010675:Pheromones / Q000235:genetics / Q000378:metabolism; D034443:RNA Transport; D012333:RNA, Messenger / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D008074:Lipoproteins; C095063:MFA2 protein, S cerevisiae; D010675:Pheromones; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins