title: Detection of SPM-1-producing Pseudomonas aeruginosa and class D β-lactamase-producing Acinetobacter baumannii isolates by use of liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization-time of flight mass spectrometry.
abstract: This study evaluates the accuracy of liquid chromatography-mass spectrometry (LC-MS) and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF) for detecting carbapenem hydrolytic activity among SPM-1-, GIM-1-, and GES-5-producing Pseudomonas aeruginosa isolates and OXA-143-, IMP-10-, and OXA-58-producing Acinetobacter baumannii isolates. Class A and B carbapenemase activities were rapidly detected by MALDI-TOF in a 2-h assay. However, an extended incubation time was necessary for detection of carbapenem-hydrolyzing class D β-lactamase (CHDL) activity in Acinetobacter spp.
authors.affiliation: Laboratório Alerta, Disciplina de Infectologia, Departamento de Medicina, Universidade Federal de São Paulo (UNIFESP), São Paulo, São Paulo, Brazil. cicacarvalhaes@gmail.com
authors.forename: Cecilia G
authors.initials: CG
authors.lastname: Carvalhaes
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Cayô
authors.forename: Diego M
authors.initials: DM
authors.lastname: Assis
authors.forename: Evelin R
authors.initials: ER
authors.lastname: Martins
authors.forename: Luiz
authors.initials: L
authors.lastname: Juliano
authors.forename: Maria A
authors.initials: MA
authors.lastname: Juliano
authors.forename: Ana C
authors.initials: AC
authors.lastname: Gales
mesh_terms: D040981:Acinetobacter baumannii / Q000737:chemistry* / Q000201:enzymology* / Q000302:isolation & purification; D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000201:enzymology* / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D001618:beta-Lactamases