title: Enhanced architecture of lipid-carbon nanotubes as langmuir-blodgett films to investigate the enzyme activity of phospholipases from snake venom.
abstract: The immobilization of biomolecules in films with a controlled architecture permits the access of information on the molecular interactions, not only between film components, but also between the film and substances in the external environment. In this study, we investigated the immobilization of the phospholipase A(2) from snake venoms (4-nitro-3-(octanoyloxy)benzoic acid, OBZ) in solid supports as a Langmuir-Blodgett (LB) film, followed by incorporation of carbon nanotubes (CNTs). The hybrid film was characterized by infrared spectroscopy and the interactions with its catalytic substrate were investigated. The presence of CNTs leads to a structure with an adequate environment to preserve the enzyme properties, leading to an optimum catalytic activity. This enhanced architecture was exploited in terms of vibrational spectroscopy, which indicated changes in the secondary structure of the enzyme upon contact with the catalytic substrate.
authors.affiliation: Federal University of São Paulo (UNIFESP), Diadema, São Paulo (SP), 09972-27, Brazil. lcaseli@unifesp.br
authors.forename: Luciano
authors.initials: L
authors.lastname: Caseli
authors.forename: Vera L B
authors.initials: VL
authors.lastname: Tiburcio
authors.forename: Frey F R
authors.initials: FF
authors.lastname: Vargas
authors.forename: Sérgio
authors.initials: S
authors.lastname: Marangoni
authors.forename: José R
authors.initials: JR
authors.lastname: Siqueira
mesh_terms: D055162:Biocatalysis; D008055:Lipids / Q000737:chemistry*; D037742:Nanotubes, Carbon; D054467:Phospholipases A2 / Q000378:metabolism*; D012910:Snake Venoms / Q000201:enzymology*; D013379:Substrate Specificity; D013499:Surface Properties
chemical_list: D008055:Lipids; D037742:Nanotubes, Carbon; D012910:Snake Venoms; D054467:Phospholipases A2