title: The role of ROS toxicity in spontaneous aneuploidy in cultured cells.
abstract: It is well known that the karyotype of animal cells cultured in vitro tends to become aneuploid as the culture ages. Aneuploidy can cause genetic instability, alter the biological properties of cells, and affect their application in genetic studies and cell engineering. Understanding the causes and mechanisms of aneuploidy is primary to control its occurrence in cultured cells, and is also helpful to understand the mechanisms of tumorigenesis because aneuploidy is a hallmark of tumor cells. This review underscores the potential role of reactive oxygen species (ROS) toxicity in spontaneous aneuploidy of cultured cells. The underlying mechanisms and possible sources of ROS are also discussed.
authors.affiliation: Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, Research Institute of Resource Insects, Chinese Academy of Forestry, Kunming 650224, China. chengyewang@yahoo.com.cn
authors.forename: Cheng-Ye
authors.initials: CY
authors.lastname: Wang
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Liu
authors.forename: Zhong-Bao
authors.initials: ZB
authors.lastname: Zhao
mesh_terms: D000375:Aging / Q000187:drug effects*; D000782:Aneuploidy; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D059785:Karyotype; D017382:Reactive Oxygen Species / Q000633:toxicity*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
chemical_list: D017382:Reactive Oxygen Species; D016159:Tumor Suppressor Protein p53