title: Human recombinant elastin-like protein coatings for muscle cell proliferation and differentiation.
abstract: Recombinant proteins represent a new and promising class of polymeric materials in the field of biomaterials research. An important model for biomaterial design is elastin, the protein accounting for the elasticity of several tissues. Human elastin-like polypeptides (HELPs) have been developed as recombinant versions of elastin with the purpose of enhancing some peculiar characteristics of the native protein, like self-assembling. In this paper, we report on a comparative study of rat myoblasts response to coatings based on two different HELP macromolecules, with respect to control cultures on bare cell culture polystyrene and on a standard collagen coating. Cell behavior was analyzed in terms of adhesion, proliferation and differentiation. The collected data strongly suggest the use of HELPs as excellent biomaterials for tissue engineering and regenerative medicine applications.
authors.affiliation: Istituto Italiano di Tecnologia, Center for Micro-BioRobotics @SSSA, Viale Rinaldo Piaggio 34, 56025 Pontedera, Pisa, Italy. gianni.ciofani@iit.it
authors.forename: Gianni
authors.initials: G
authors.lastname: Ciofani
authors.forename: Giada G
authors.initials: GG
authors.lastname: Genchi
authors.forename: Ioannis
authors.initials: I
authors.lastname: Liakos
authors.forename: Athanassia
authors.initials: A
authors.lastname: Athanassiou
authors.forename: Virgilio
authors.initials: V
authors.lastname: Mattoli
authors.forename: Antonella
authors.initials: A
authors.lastname: Bandiera
mesh_terms: D000199:Actins / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D040761:Biomimetic Materials / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects* / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D004549:Elastin / Q000737:chemistry / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008969:Molecular Sequence Data; D032342:Muscle Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D017570:MyoD Protein / Q000235:genetics / Q000378:metabolism; D018008:Myogenin / Q000235:genetics / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000235:genetics / Q000378:metabolism; D056951:Photoelectron Spectroscopy; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology*; D016596:Vinculin / Q000378:metabolism
chemical_list: D000199:Actins; D020099:Coated Materials, Biocompatible; D017570:MyoD Protein; D018008:Myogenin; D011994:Recombinant Proteins; D016596:Vinculin; D004549:Elastin; D018995:Myosin Heavy Chains