title: Differences in neuropeptide Y-induced secretion of endothelin-1 in left and right human endocardial endothelial cells.
abstract: The aim of the study was to test the hypothesis that neuropeptide Y (NPY) may induce endothelin-1 (ET-1) secretion in left (hLEECs) and right (hREECs) human endocardial endothelial cells. Furthermore, the type of NPY receptor implicated could be different in NPY-induced secretion in hLEECs and hREECs. Using immunofluorescence coupled to real 3D confocal microscopy and ELISA, our results showed that stimulation of secretion by NPY induced the release of ET-1 from both right and left human ventricular endocardial endothelial cells (hEECs) in a time-dependent manner. Furthermore, the secretory capacity of hREECs was higher than that of hLEECs. In addition, our results showed that the effect of NPY on ET-1 secretion in hLEECs was only due to activation of Y(5) receptors. However, the effect of NPY on ET-1 secretion in hREECs was due to mainly Y(2) and partially Y(5) receptors activation. In conclusion, our results suggest that differences in excitation-secretion coupling exist between hREECS and hLEECs which may contribute to the functional differences between right and left ventricular muscle. Furthermore, high NPY level contributes to ET-1 release by hEECs and Y(2) and Y(5) receptors antagonists may be used for regulation of ET-1 secretion in the heart.
authors.affiliation: Department of Anatomy and Cell Biology, Faculty of Medicine, University of Sherbrooke, Sherbrooke, Quebec, Canada J1H 5N4.
authors.forename: Dima
authors.initials: D
authors.lastname: Abdel-Samad
authors.forename: Claudine
authors.initials: C
authors.lastname: Perreault
authors.forename: Lena
authors.initials: L
authors.lastname: Ahmarani
authors.forename: Levon
authors.initials: L
authors.lastname: Avedanian
authors.forename: Ghassan
authors.initials: G
authors.lastname: Bkaily
authors.forename: Sheldon
authors.initials: S
authors.lastname: Magder
authors.forename: Pedro
authors.initials: P
authors.lastname: D'Orléans-Juste
authors.forename: Danielle
authors.initials: D
authors.lastname: Jacques
mesh_terms: D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D004699:Endocardium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D019332:Endothelin-1 / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D009478:Neuropeptide Y / Q000378:metabolism / Q000494:pharmacology*; D011863:Radioimmunoassay; D017476:Receptors, Neuropeptide Y / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000502:physiology
chemical_list: D019332:Endothelin-1; D009478:Neuropeptide Y; D017476:Receptors, Neuropeptide Y; D001120:Arginine; C117416:BIBO 3304