title: Application of visible light-based projection stereolithography for live cell-scaffold fabrication with designed architecture.
abstract: One-step scaffold fabrication with live cell incorporation is a highly desirable technology for tissue engineering and regeneration. Projection stereolithography (PSL) represents a promising method owing to its fine resolution, high fabrication speed and computer-aided design (CAD) capabilities. However, the majority of current protocols utilize water-insoluble photoinitiators that are incompatible with live cell-fabrication, and ultraviolet (UV) light that is damaging to the cellular DNA. We report here the development of a visible light-based PSL system (VL-PSL), using lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) as the initiator and polyethylene glycol diacrylate (PEGDA) as the monomer, to produce hydrogel scaffolds with specific shapes and internal architectures. Furthermore, live human adipose-derived stem cells (hADSCs) were suspended in PEGDA/LAP solution during the PSL process, and were successfully incorporated within the fabricated hydrogel scaffolds. hADSCs in PEG scaffolds showed high viability (>90%) for up to 7 days after fabrication as revealed by Live/Dead staining. Scaffolds with porous internal architecture retained higher cell viability and activity than solid scaffolds, likely due to increased oxygen and nutrients exchange into the interior of the scaffolds. The VL-PSL should be applicable as an efficient and effective tissue engineering technology for point-of-care tissue repair in the clinic.
authors.affiliation: Center for Cellular and Molecular Engineering, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA.
authors.forename: Hang
authors.initials: H
authors.lastname: Lin
authors.forename: Dongning
authors.initials: D
authors.lastname: Zhang
authors.forename: Peter G
authors.initials: PG
authors.lastname: Alexander
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.forename: Jian
authors.initials: J
authors.lastname: Tan
authors.forename: Anthony Wai-Ming
authors.initials: AW
authors.lastname: Cheng
authors.forename: Rocky S
authors.initials: RS
authors.lastname: Tuan
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology; D002470:Cell Survival; D002478:Cells, Cultured; D017076:Computer-Aided Design; D005260:Female; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008027:Light; D010721:Phosphinic Acids / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D010721:Phosphinic Acids; C546776:phenyl-2,4,6-trimethylbenzoylphosphinate; C437167:poly(ethylene glycol)diacrylate; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols