title: CINtec PLUS immunocytochemistry as a tool for the cytologic diagnosis of glandular lesions of the cervix uteri.
abstract: Cytologic findings of glandular lesions of the cervix uteri are often difficult to evaluate. We studied the usefulness of CINtec PLUS p16/Ki-67 double stain (mtm laboratories, Heidelberg, Germany) for the diagnosis of glandular lesions. The study included 47 abnormal results on liquid-based cytologic tests with a subsequent histologic diagnosis of adenocarcinoma in situ or with early invasion, and 16 samples with negative results on follow-up. All samples were stained with CINtec PLUS p16/Ki-67 double stain. Of the neoplastic samples, 7 were excluded because of insufficient residual cellularity or loss of neoplastic cells. Of the samples that were adequate, 92.5% were stained with CINtec PLUS, whereas 7.5% were judged inconclusive. All inconclusive cases were at least 3 years old. Of the 16 negative samples, 15 (93.8%) stained negative and only 1 (6.2%) showed several positive clusters of cells. Our study shows that CINtec PLUS is a robust and useful tool for the diagnosis of glandular lesions of the cervix uteri.
authors.affiliation: Dept of Pathology, University of Cagliari, AOU San Giovanni di Dio, via Ospedale 54, 09124 Cagliari, Italy. albertoravarino@tiscali.it
authors.forename: Alberto
authors.initials: A
authors.lastname: Ravarino
authors.forename: Sonia
authors.initials: S
authors.lastname: Nemolato
authors.forename: Elena
authors.initials: E
authors.lastname: Macciocu
authors.forename: Matteo
authors.initials: M
authors.lastname: Fraschini
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Senes
authors.forename: Gavino
authors.initials: G
authors.lastname: Faa
authors.forename: Giovanni
authors.initials: G
authors.lastname: Negri
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D002584:Cervix Uteri / Q000378:metabolism / Q000473:pathology*; D003584:Cytological Techniques; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology