title: Resveratrol attenuates L-DOPA-induced hydrogen peroxide toxicity in neuronal cells.
abstract: A variety of polyphenol antioxidant compounds derived from natural products have demonstrated neuroprotective activity against neuronal cell death. The objective of this study was to investigate the effect of resveratrol (RESV) and bioflavonoids in attenuating hydrogen peroxide (H(2)O(2))-induced oxidative stress in neuronal cells. H2O2 levels were increased by the addition of L-3,4-dihydroxyphenylalanine (L-DOPA) to cultured dopaminergic SKNSH cells. H(2)O(2) was monitored by peroxyfluor-1, a selective H(2)O(2) optical probe. To examine the neuroprotective effects of RESV and bioflavonoids against L-DOPA, we cotreated RESV, quercetin, or (-) epigallocatechin gallate with L-DOPA and monitored for H(2)O(2) levels. The combination of RESV and L-DOPA was 50% more effective at reducing H(2)O(2) levels than the combination of quercetin or epigallocatechin gallate with L-DOPA. However, the combination of each antioxidant with L-DOPA was effective at preserving cell viability.
authors.affiliation: Department of aChemistry bBiology, Boston University, Boston, Massachusetts 02215, USA.
authors.forename: Carina S
authors.initials: CS
authors.lastname: Peritore
authors.forename: Angela
authors.initials: A
authors.lastname: Ho
authors.forename: Bryan K
authors.initials: BK
authors.lastname: Yamamoto
authors.forename: Scott E
authors.initials: SE
authors.lastname: Schaus
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives / Q000494:pharmacology; D045744:Cell Line, Tumor / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D004347:Drug Interactions; D005347:Fibroblasts / Q000187:drug effects; D005452:Fluoresceins / Q000032:analysis; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D007980:Levodopa / Q000037:antagonists & inhibitors* / Q000633:toxicity; D008995:Monoamine Oxidase / Q000032:analysis; D009419:Nerve Tissue Proteins / Q000032:analysis; D009447:Neuroblastoma / Q000473:pathology; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D011794:Quercetin / Q000494:pharmacology; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D014443:Tyrosine / Q000494:pharmacology; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
chemical_list: D000975:Antioxidants; D005452:Fluoresceins; D009419:Nerve Tissue Proteins; D018696:Neuroprotective Agents; D013267:Stilbenes; C500013:peroxyfluor-1; D014443:Tyrosine; D007980:Levodopa; D002392:Catechin; D011794:Quercetin; D006861:Hydrogen Peroxide; C045651:epigallocatechin gallate; D014446:Tyrosine 3-Monooxygenase; D008995:Monoamine Oxidase; D000077185:Resveratrol