title: Injectable collagen/α-tricalcium phosphate cement: collagen-mineral phase interactions and cell response.
abstract: A bone inspired material was obtained by incorporating collagen in the liquid phase of an α-tricalcium phosphate cement, either in solubilized or in fibrilized form. This material was able to set in situ, giving rise to a calcium deficient hydroxyapatite (CDHA)/collagen composite. The morphology and distribution of collagen in the composite was shown to be strongly affected by the collagen pre-treatment. The interactions between collagen and the inorganic phase were assessed by FTIR. A red shift of the amide I band was indicative of calcium chelation by the collagen carbonyl groups. The rate of CDHA formation was not affected when diluted collagen solutions (1 mg/ml) were used, whereas injectability improved. The presence of solubilized collagen, even in low amount (1 %), increased cell adhesion and proliferation on the composites. Still in the absence of osteogenic medium, significant ALP activity was detected both in the inorganic and the collagen-containing cements. The maximum ALP activity was advanced in the collagen-containing cement as compared to the inorganic cement.
authors.affiliation: Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgy, Technical University of Catalonia (UPC), Avda. Diagonal 647, 08028, Barcelona, Spain.
authors.forename: Roman A
authors.initials: RA
authors.lastname: Perez
authors.forename: Maria-Pau
authors.initials: MP
authors.lastname: Ginebra
mesh_terms: D001031:Apatites / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D001696:Biomechanical Phenomena / Q000187:drug effects; D001843:Bone Cements / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000008:administration & dosage / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D004338:Drug Combinations; D017886:Durapatite / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D007267:Injections; D008422:Materials Testing; D008903:Minerals / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology
chemical_list: D001031:Apatites; D001843:Bone Cements; D002130:Calcium Phosphates; D004338:Drug Combinations; D008903:Minerals; C485828:alpha-tricalcium phosphate; C040699:calcium hydroxyapatite, collagen drug combination; D003094:Collagen; D017886:Durapatite