title: Enhanced production of (+)-terrein in fed-batch cultivation of Aspergillus terreus strain PF26 with sodium citrate.
abstract: (+)-Terrein is a fungal metabolite with multiple biological activities, especially with great value in medicine. However, the mass production of single configuration terrein is still a big challenge. In this study, the effects of acetic acid, sodium acetate, citric acid and sodium citrate on the (+)-terrein production by Aspergillus terreus strain PF26 derived from marine sponge Phakellia fusca were investigated. Sodium citrate was selected for fed-batch cultivation because it showed the best effect on (+)-terrein production among the four regulators tested. As a result, 5.38 g/L (+)-terrein production was achieved by feeding 10 mM sodium citrate on the 3rd day in shake flask, which was 33.8 % higher than the control and represented the highest yield of (+)-terrein. In a 7.5-L stirred bioreactor, 2.58 g/L of (+)-terrein production was achieved by the feeding of 10 mM sodium citrate on the 8th day. The results from this study lay a basis for the high-yield production of (+)-terrein by fermentation.
authors.affiliation: Marine Biotechnology Laboratory, State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yin
authors.forename: Bailiang
authors.initials: B
authors.lastname: Xu
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Li
authors.forename: Baohua
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001230:Aspergillus / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*; D018533:Biomass; D019149:Bioreactors; D002951:Citrates / Q000378:metabolism; D003470:Culture Media; D003517:Cyclopentanes / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D011161:Porifera / Q000382:microbiology*; D000077559:Sodium Citrate
chemical_list: D002951:Citrates; D003470:Culture Media; D003517:Cyclopentanes; D000077559:Sodium Citrate; C439087:terrein