title: Characterization of 21 microsatellite markers from cogongrass, Imperata cylindrica (Poaceae), a weed species distributed worldwide.
abstract: OBJECTIVE
  Microsatellite loci were developed from Imperata cylindrica, a traditional medicinal herb in Asia and among the top 10 worst invasive weeds in the world, to aid in the identification of the limits of asexual clonal individuals.
  
  
  RESULTS
  A total of 21 microsatellite markers, including 18 polymorphic and three monomorphic loci, were developed from I. cylindrica using a magnetic bead enrichment protocol. The primers amplified dinucleotide, trinucleotide, and complex repeats. The number of alleles ranged from one to 19 per locus, with an observed heterozygosity ranging from 0.09 to 1.00. Several loci deviated significantly from the within-population Hardy-Weinberg equilibrium as a result of asexual clonal reproduction.
  
  
  CONCLUSIONS
  These polymorphic markers should be useful tools in further studies on the identification of the range of clonal reproduction units and the selection and classification of the medicinal cultivar.
authors.affiliation: Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
authors.forename: Yu-Chung
authors.initials: YC
authors.lastname: Chiang
authors.forename: Chi-Chu
authors.initials: CC
authors.lastname: Tsai
authors.forename: Tsai-Wen
authors.initials: TW
authors.lastname: Hsu
authors.forename: Chang-Hung
authors.initials: CH
authors.lastname: Chou
mesh_terms: D001483:Base Sequence; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D005843:Geography; D007564:Japan; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D057685:Plant Weeds / Q000235:genetics*; D010946:Plants, Medicinal / Q000235:genetics*; D006109:Poaceae / Q000235:genetics*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013624:Taiwan
chemical_list: D018744:DNA, Plant