title: A mouse model of Salmonella typhi infection.
abstract: Salmonella spp. are gram-negative flagellated bacteria that can cause food- and waterborne gastroenteritis and typhoid fever in humans. We now report that flagellin from Salmonella spp. is recognized in mouse intestine by Toll-like receptor 11 (TLR11). Absence of TLR11 renders mice more susceptible to infection by S. Typhimurium, with increased dissemination of the bacteria and enhanced lethality. Unlike S. Typhimurium, S. Typhi, a human obligatory pathogen that causes typhoid fever, is normally unable to infect mice. TLR11 is expressed in mice, but not in humans, and remarkably, we find that tlr11(-/-) mice are efficiently infected with orally administered S. Typhi. We also find that tlr11(-/-) mice can be immunized against S. Typhi. Therefore, tlr11(-/-) mice represent a small-animal model for the study of the immune response to S. Typhi and for the development of vaccines against this important human pathogen.
authors.affiliation: Department of Microbiology and Immunology, Columbia University, College of Physicians and Surgeons, New York, NY 10032, USA.
authors.forename: Ramkumar
authors.initials: R
authors.lastname: Mathur
authors.forename: Hyunju
authors.initials: H
authors.lastname: Oh
authors.forename: Dekai
authors.initials: D
authors.lastname: Zhang
authors.forename: Sung-Gyoo
authors.initials: SG
authors.lastname: Park
authors.forename: Jin
authors.initials: J
authors.lastname: Seo
authors.forename: Alicia
authors.initials: A
authors.lastname: Koblansky
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Hayden
authors.forename: Sankar
authors.initials: S
authors.lastname: Ghosh
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005408:Flagellin / Q000378:metabolism; D054884:Host-Pathogen Interactions; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012485:Salmonella typhi; D013045:Species Specificity; D051193:Toll-Like Receptors / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014435:Typhoid Fever / Q000276:immunology* / Q000382:microbiology*
chemical_list: C482903:Tlr11 protein, mouse; D051193:Toll-Like Receptors; D005408:Flagellin