title: Maturation and upregulation of functions of murine dendritic cells (DCs) under the influence of purified aromatic-turmerone (AR).
abstract: The aim of this work is to evaluate the effects of purified aromatic-turmerone (ar-turmerione, AR) on murine dendritic cells (DCs). These impacts of AR on DCs from bone marrow derived DCs(BMDCs) were assessed with use of conventional scanning electron microscopy (SEM), fluorescence activated cell sorting (FACS), transmission electron microscopy (TEM), cytochemistry assay, FITC-dextran, bio-assay and enzyme linked immunosorbent assay (ELISA). We found that AR induced phenotypic maturation as evidenced by increased expression of CD86, CD40, CD83, CD80 and major histocompatibility complex II (MHC II). The functional tests showed the activity of acidic phosphatase (ACP) inside the DCs were downregulated after treatment with AR (which occurs when phagocytosis of DCs were decreased). Finally, we proved that AR increased the production of IL-12 and tumor necrosis factor α (TNF-α). These data suggested that AR could promote phenotypic and functional maturation of DCs and this adjuvant-like activity may have potential therapeutic value. It is therefore concluded that AR could exert positive modulation on murine DCs.
authors.affiliation: Department of Immunology, School of Basic Medical Science, China Medical University, Heping District, Shenyang, PR China.
authors.forename: Tan
authors.initials: T
authors.lastname: Yonggang
authors.forename: Meng
authors.initials: M
authors.lastname: Yiming
authors.forename: Zhang
authors.initials: Z
authors.lastname: Heying
authors.forename: Sun
authors.initials: S
authors.lastname: Cheng
authors.forename: Wang
authors.initials: W
authors.lastname: Qiushi
authors.forename: Yang
authors.initials: Y
authors.lastname: Xianghong
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wei
authors.forename: Zhou
authors.initials: Z
authors.lastname: Huawei
authors.forename: Fengping
authors.initials: F
authors.lastname: Shan
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D019013:CD40 Antigens / Q000378:metabolism; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D007136:Immunoglobulins / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D007659:Ketones / Q000494:pharmacology*; D008285:Major Histocompatibility Complex; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D012717:Sesquiterpenes / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D019013:CD40 Antigens; D007136:Immunoglobulins; D018664:Interleukin-12; D007659:Ketones; D008562:Membrane Glycoproteins; D012717:Sesquiterpenes; D014409:Tumor Necrosis Factor-alpha; D000097802:CD83 Antigen; C078098:ar-turmerone
keywords: AR; BMDCs; maturation; modulation