title: Lack of association between genetic polymorphisms within KCNQ1 locus and type 2 diabetes in Tunisian Arabs.
abstract: OBJECTIVE
  Polymorphisms of KCNQ1 were previously associated with type 2 diabetes (T2DM) in select Caucasian and non-Caucasian populations. We investigated the association of rs231361, rs231359, rs151290, rs2237892, rs2283228, rs2237895, and rs2237896 KCNQ1 polymorphisms with T2DM in Tunisian Arabs.
  
  
  METHODS
  Subjects comprised 900 T2DM patients and 600 normoglycemic controls. KCNQ1 genotyping was done by allelic discrimination (real-time PCR) and PCR-RFLP methods; the contribution of KCNQ1 polymorphisms to T2DM were analyzed by Haploview and regression analysis.
  
  
  RESULTS
  Minor allele frequency (MAF) of the 7 tested KCNQ1 variants was comparable between T2DM cases and controls. Mild association of rs2237892 genotypes with T2DM was seen (P=0.014), highlighted by the significant association of the C/T genotype with increased T2DM risk (OR, 2.11; 95%CI, 1.25-3.53), after adjusting for BMI, gender, systolic and diastolic blood pressure, and serum lipid profile. Heterogeneity in linkage disequilibrium pattern between tested KCNQ1 variants analyzed was seen. Two-locus (rs231361 and rs231359) and 5-locus (remaining 5 SNPs) haplotype analysis did not reveal any significant association with any of the haplotypes contained in either block 1 or block 2.
  
  
  CONCLUSIONS
  These results indicate that there was no evidence for an association of KCNQ1 polymorphisms with T2DM in Tunisian Arabs.
authors.affiliation: Research Unit of Biology and Genetics of Cancer and Haematological and Autoimmune Diseases, Faculty of Pharmacy of Monastir, University of Monastir, Monastir, Tunisia.
authors.forename: Amira
authors.initials: A
authors.lastname: Turki
authors.forename: Nabil
authors.initials: N
authors.lastname: Mtiraoui
authors.forename: Amna S
authors.initials: AS
authors.lastname: Al-Busaidi
authors.forename: Moncef
authors.initials: M
authors.lastname: Khirallah
authors.forename: Touhami
authors.initials: T
authors.lastname: Mahjoub
authors.forename: Wassim Y
authors.initials: WY
authors.lastname: Almawi
mesh_terms: D000368:Aged; D000483:Alleles; D018912:Arabs; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D006239:Haplotypes; D006801:Humans; D051657:KCNQ1 Potassium Channel / Q000235:genetics* / Q000378:metabolism; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D010044:Outpatient Clinics, Hospital; D020641:Polymorphism, Single Nucleotide; D014416:Tunisia
chemical_list: D051657:KCNQ1 Potassium Channel; C496909:KCNQ1 protein, human