title: Complete nucleotide sequence of the first KPC-2- and SHV-12-encoding IncX plasmid, pKpS90, from Klebsiella pneumoniae.
abstract: We report the complete nucleotide sequence of the pKpS90 plasmid, carrying the bla(KPC-2) and bla(SHV-12) genes. This plasmid was isolated from a sequence type 258 (ST258) Klebsiella pneumoniae strain responsible for an outbreak in a French university hospital in 2009. pKpS90 is a 53,286-bp plasmid that belongs to the IncX incompatibility group. pKpS90 consists of a backbone from IncX plasmids, in which the KPC-2-encoding Tn4401 transposon and a bla(SHV-12)-encoding region have been inserted.
authors.affiliation: Laboratoire de Bactériologie, Hôpital Paul Brousse, AP-HP, Villejuif, France.
authors.forename: Najiby
authors.initials: N
authors.lastname: Kassis-Chikhani
authors.forename: Lionel
authors.initials: L
authors.lastname: Frangeul
authors.forename: Laurence
authors.initials: L
authors.lastname: Drieux
authors.forename: Christian
authors.initials: C
authors.lastname: Sengelin
authors.forename: Vincent
authors.initials: V
authors.lastname: Jarlier
authors.forename: Sylvain
authors.initials: S
authors.lastname: Brisse
authors.forename: Guillaume
authors.initials: G
authors.lastname: Arlet
authors.forename: Dominique
authors.initials: D
authors.lastname: Decré
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001483:Base Sequence; D004251:DNA Transposable Elements; D004196:Disease Outbreaks; D005602:France / Q000453:epidemiology; D006785:Hospitals, University; D006801:Humans; D007710:Klebsiella Infections / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids; D017422:Sequence Analysis, DNA; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D047090:beta-Lactams; C107198:beta-lactamase SHV-12; D001618:beta-Lactamases; C547585:beta-lactamase KPC-2, Klebsiella pneumoniae