title: The genome organization of lucerne transient streak and turnip rosette sobemoviruses revisited.
abstract: Unlike other sobemoviruses, lucerne transient streak virus (LTSV) and turnip rosette virus (TRoV) have been reported to contain two successive ORF1s (denoted as ORF1a and ORF1b) instead of a single ORF1. Also, their next ORF (ORF2a/2a2b) has been mapped to a region ca. 200 nucleotides downstream from that of other sobemoviruses, leading to the lack of transmembrane segments at the N-termini of P2a/2a2b. In the current study, we resequenced this region for TRoV and LTSV. The hypothetical beginning of ORF1b was mapped as the beginning of ORF2a/2a2b for both TRoV and LTSV. Computional analysis revealed transmembrane segments at the N-termini of the TRoV and LTSV polyproteins.
authors.affiliation: Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia. merike.somera@ttu.ee
authors.forename: Merike
authors.initials: M
authors.lastname: Sõmera
authors.forename: Erkki
authors.initials: E
authors.lastname: Truve
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D029688:Brassica napus / Q000821:virology*; D016679:Genome, Viral / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010942:Plant Viruses / Q000235:genetics*; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA
chemical_list: D012367:RNA, Viral