title: Ultrasensitive one-step rapid detection of ochratoxin A by the folding-based electrochemical aptasensor.
abstract: A one-step electrochemical aptasensor using the thiol- and methylene blue- (MB-) dual-labeled aptamer modified gold electrode for determination of ochratoxin A (OTA) was presented in this research. The aptamer against OTA was covalently immobilized on the surface of the electrode by the self-assembly effect and used as recognition probes for OTA detection by the binding induced folding of the aptamer. Under the optimal conditions, the developed electrochemical aptasensor demonstrated a wide linear range from 0.1 pg mL(-1) to 1000 pg mL(-1) with the limit of detection (LOD) of 0.095 pg mL(-1), which was an extraordinary sensitivity compared with other common methods for OTA detection. Moreover, as a practical application, this proposed electrochemical aptasensor was used to monitor the OTA level in red wine samples without any special pretreatment and with satisfactory results obtained. Study results showed that this electrochemical aptasensor could be a potential useful platform for on-site OTA measurement in real complex samples.
authors.affiliation: School of Biotechnology & Food Engineering, Hefei University of Technology, Hefei, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wu
authors.forename: Huaqin
authors.initials: H
authors.lastname: Chu
authors.forename: Zhanlong
authors.initials: Z
authors.lastname: Mei
authors.forename: Yi
authors.initials: Y
authors.lastname: Deng
authors.forename: Feng
authors.initials: F
authors.lastname: Xue
authors.forename: Lei
authors.initials: L
authors.lastname: Zheng
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold; D008751:Methylene Blue / Q000737:chemistry; D009793:Ochratoxins / Q000032:analysis*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D014920:Wine / Q000032:analysis
chemical_list: D052157:Aptamers, Nucleotide; D009793:Ochratoxins; D013438:Sulfhydryl Compounds; C025589:ochratoxin A; D006046:Gold; D008751:Methylene Blue