title: Influence of in ovo prebiotic and synbiotic administration on meat quality of broiler chickens.
abstract: A trial was conducted to evaluate the effect of in ovo injection of prebiotic and synbiotics on growth performance, meat quality traits (cholesterol content, intramuscular collagen properties, fiber measurements), and the presence of histopathological changes in the pectoral muscle (PS) of broiler chickens. On d 12 of incubation, 480 eggs were randomly divided into 5 experimental groups treated with different bioactives, in ovo injected: C, control with physiological saline; T1 with 1.9 mg of raffinose family oligosaccharides; T2 and T3 with 1.9 mg of raffinose family oligosaccharides enriched with different probiotic bacteria, specifically 1,000 cfu of Lactococcus lactis ssp. lactis SL1 and Lactococcus lactis ssp. cremoris IBB SC1, respectively; T4 with commercially available synbiotic Duolac, containing 500 cfu of both Lactobacillus acidophilus and Streptococcus faecium with the addition of lactose (0.001 mg/embryo). Among the hatched chickens, 60 males were randomly chosen (12 birds for each group) and were grown to 42 d in collective cages (n = 3 birds in each 4 cages: replications for experimental groups). Broilers were fed ad libitum commercial diets according to age. In ovo prebiotic and synbiotic administration had a low effect on investigated traits, but depend on the kind of bioactives administered. Commercial synbiotic treatment (T4) reduced carcass yield percentage, and the feed conversion ratio was higher in T3 and T4 groups compared with other groups. The abdominal fat, the ultimate pH, and cholesterol of the PS were not affected by treatment. Broiler chickens of the treated groups with both slightly greater PS and fiber diameter had a significantly lower amount of collagen. The greater thickness of muscle fibers (not significant) and the lower fiber density (statistically significant), observed in treated birds in comparison with those of the C group, are not associated with histopathological changes in the PS of broilers. The incidence of histopathological changes in broiler chickens from examined groups was low, which did not affect the deterioration of meat quality obtained from these birds.
authors.affiliation: Department of Agricultural, Environmental and Food Sciences, University of Molise, Campobasso, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Maiorano
authors.forename: A
authors.initials: A
authors.lastname: Sobolewska
authors.forename: D
authors.initials: D
authors.lastname: Cianciullo
authors.forename: K
authors.initials: K
authors.lastname: Walasik
authors.forename: G
authors.initials: G
authors.lastname: Elminowska-Wenda
authors.forename: A
authors.initials: A
authors.lastname: Slawinska
authors.forename: S
authors.initials: S
authors.lastname: Tavaniello
authors.forename: J
authors.initials: J
authors.lastname: Zylinska
authors.forename: J
authors.initials: J
authors.lastname: Bardowski
authors.forename: M
authors.initials: M
authors.lastname: Bednarczyk
mesh_terms: D000818:Animals; D001823:Body Composition; D002642:Chick Embryo; D002645:Chickens; D002784:Cholesterol / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008460:Meat / Q000032:analysis / Q000592:standards*; D018482:Muscle, Skeletal / Q000737:chemistry / Q000502:physiology; D010063:Ovum; D056692:Prebiotics; D058616:Synbiotics
chemical_list: D056692:Prebiotics; D002784:Cholesterol