title: Immune parameters correlate with protection against ebola virus infection in rodents and nonhuman primates.
abstract: Ebola virus causes severe hemorrhagic fever in susceptible hosts. Currently, no licensed vaccines or treatments are available; however, several experimental vaccines have been successful in protecting rodents and nonhuman primates (NHPs) from the lethal Zaire ebolavirus (ZEBOV) infection. The objective of this study was to evaluate immune responses correlating with survival in these animals after lethal challenge with ZEBOV. Knockout mice with impaired ability to generate normal T and/or B cell responses were vaccinated and challenged with ZEBOV. Vaccine-induced protection in mice was mainly mediated by B cells and CD4(+) T cells. Vaccinated, outbred guinea pigs and NHPs demonstrated the highest correlation between survival and levels of total immunoglobulin G (IgG) specific to the ZEBOV glycoprotein (ZGP). These results highlight the relevance of total ZGP-specific IgG levels as a meaningful correlate of protection against ZEBOV exposure.
authors.affiliation: Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba R3E 3R2, Canada.
authors.forename: Gary
authors.initials: G
authors.lastname: Wong
authors.forename: Jason S
authors.initials: JS
authors.lastname: Richardson
authors.forename: Stéphane
authors.initials: S
authors.lastname: Pillet
authors.forename: Ami
authors.initials: A
authors.lastname: Patel
authors.forename: Xiangguo
authors.initials: X
authors.lastname: Qiu
authors.forename: Judie
authors.initials: J
authors.lastname: Alimonti
authors.forename: Jeff
authors.initials: J
authors.lastname: Hogan
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ayato
authors.initials: A
authors.lastname: Takada
authors.forename: Heinz
authors.initials: H
authors.lastname: Feldmann
authors.forename: Gary P
authors.initials: GP
authors.lastname: Kobinger
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D029043:Ebolavirus / Q000472:pathogenicity*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006168:Guinea Pigs; D019142:Hemorrhagic Fever, Ebola / Q000276:immunology*; D051379:Mice; D011323:Primates / Q000276:immunology* / Q000821:virology*