title: Lesion of olfactory epithelium attenuates expression of morphine-induced behavioral sensitization and reinstatement of drug-primed conditioned place preference in mice.
abstract: Previous studies have shown that olfactory impairment by disrupting the olfactory epithelium prior to morphine administration attenuated the development addiction-related behaviors. However, it is unclear whether olfactory impairment will affect the expression of already established addiction-related behaviors. To address this issue, mice were conditioned with morphine to induce behavioral sensitization and condition placed preference (CPP). After an abstinence period, the animals were subjected to either an intranasal ZnSO(4) effusion (ZnE) or sham treatment with saline. Behavioral sensitization and CPP reinstatement were evaluated 24h later, as well as the expression of c-Fos protein, a marker of activated neural sites, in brain regions of interest. It was found that ZnE treatment attenuated morphine-induced behavioral sensitization and reinstatement of CPP. Compared to the saline-treated ones, the ZnE-treated animals showed reduced c-Fos expression in the nucleus accumbens (NAc) associated with behavioral sensitization, and in the NAc, cingulate cortex, dentate gyrus, amygdala, lateral hypothalamus and ventral tegmental area associated with CPP reinstatement. Together, these results demonstrated that acute olfactory impairment could attenuate already established addiction-related behaviors and expression of c-Fos in drug addiction related brain regions, perhaps by affecting the coordination between reward and motivational systems in the brain.
authors.affiliation: Wuhan Center for Magnetic Resonance, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 43007, PR China.
authors.forename: Haichen
authors.initials: H
authors.lastname: Niu
authors.forename: Yingwei
authors.initials: Y
authors.lastname: Zheng
authors.forename: Tanzeel
authors.initials: T
authors.lastname: Huma
authors.forename: Joshua D
authors.initials: JD
authors.lastname: Rizak
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Guimei
authors.initials: G
authors.lastname: Wang
authors.forename: He
authors.initials: H
authors.lastname: Ren
authors.forename: Liqi
authors.initials: L
authors.lastname: Xu
authors.forename: Jianzhen
authors.initials: J
authors.lastname: Yang
authors.forename: Yuanye
authors.initials: Y
authors.lastname: Ma
authors.forename: Hao
authors.initials: H
authors.lastname: Lei
mesh_terms: D000818:Animals; D016739:Behavior, Addictive / Q000139:chemically induced / Q000503:physiopathology*; D059333:Central Nervous System Sensitization / Q000187:drug effects / Q000502:physiology; D003213:Conditioning, Psychological / Q000187:drug effects / Q000502:physiology*; D004192:Discrimination, Psychological / Q000187:drug effects / Q000502:physiology; D004195:Disease Models, Animal; D005108:Extinction, Psychological / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009020:Morphine / Q000494:pharmacology*; D009043:Motor Activity; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D009831:Olfactory Mucosa / Q000187:drug effects / Q000293:injuries / Q000502:physiology*; D055696:Olfactory Perception / Q000187:drug effects / Q000502:physiology; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D019287:Zinc Sulfate
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D009020:Morphine; D019287:Zinc Sulfate