title: Identification of Prototheca zopfii from Bovine Mastitis.
abstract: BACKGROUND
  The aim of this study was identification of the epidemiology of Prototheca zopfii species from the milk samples of dairy cattle in Isfahan, central Iran.
  
  
  METHODS
  Milk samples were obtained from 230 dairy cattle, 130 with and 100 without mastitis, in Isfahan. The samples were cultured in Prototheca Isolation Medium (PIM) and Sabouraud's dextrose agar. All P. zopfii isolates were identified by morphological and biochemical methods. Then, as a confirmatory test they were examined by genotype-specific PCR.
  
  
  RESULTS
  Four P. zopfii strains (3.07%) were isolated from the 130 samples of dairy cattle with clinical mastitis and there was no isolation from totally 100 samples of healthy bovines without mastitis. Specific PCR product (about 946 bp) was detected in four isolates.
  
  
  CONCLUSIONS
  It seems that P. zopfii genotype II plays a key role in affecting bovine mastitis that confirmed other previous studies. Our study was the first, which identified the Prototheca species by traditional and molecular methods in Iran and Middle East as well.
authors.affiliation: Dept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: F
authors.initials: F
authors.lastname: Zaini
authors.forename: A
authors.initials: A
authors.lastname: Kanani
authors.forename: M
authors.initials: M
authors.lastname: Falahati
authors.forename: R
authors.initials: R
authors.lastname: Fateh
authors.forename: M
authors.initials: M
authors.lastname: Salimi-Asl
authors.forename: N
authors.initials: N
authors.lastname: Saemi
authors.forename: Sh
authors.initials: Sh
authors.lastname: Farahyar
authors.forename: A Kargar
authors.initials: AK
authors.lastname: Kheirabad
authors.forename: M
authors.initials: M
authors.lastname: Nazeri
keywords: Bovine mastitis; Iran; Prototheca zopfii