title: European surveillance for pantropic canine coronavirus.
abstract: Highly virulent pantropic canine coronavirus (CCoV) strains belonging to subtype IIa were recently identified in dogs. To assess the distribution of such strains in Europe, tissue samples were collected from 354 dogs that had died after displaying systemic disease in France (n = 92), Hungary (n = 75), Italy (n = 69), Greece (n = 87), The Netherlands (n = 27), Belgium (n = 4), and Bulgaria (n = 1). A total of 124 animals tested positive for CCoV, with 33 of them displaying the virus in extraintestinal tissues. Twenty-four CCoV strains (19.35% of the CCoV-positive dogs) detected in internal organs were characterized as subtype IIa and consequently assumed to be pantropic CCoVs. Sequence and phylogenetic analyses of the 5' end of the spike protein gene showed that pantropic CCoV strains are closely related to each other, with the exception of two divergent French viruses that clustered with enteric strains.
authors.affiliation: Department of Veterinary Medicine, University of Bari, Bari, Italy. nicola.decaro@uniba.it
authors.forename: Nicola
authors.initials: N
authors.lastname: Decaro
authors.forename: Nathalie
authors.initials: N
authors.lastname: Cordonnier
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Demeter
authors.forename: Herman
authors.initials: H
authors.lastname: Egberink
authors.forename: Gabriella
authors.initials: G
authors.lastname: Elia
authors.forename: Aurélien
authors.initials: A
authors.lastname: Grellet
authors.forename: Sophie
authors.initials: S
authors.lastname: Le Poder
authors.forename: Viviana
authors.initials: V
authors.lastname: Mari
authors.forename: Vito
authors.initials: V
authors.lastname: Martella
authors.forename: Vasileios
authors.initials: V
authors.lastname: Ntafis
authors.forename: Marcela
authors.initials: M
authors.lastname: von Reitzenstein
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Rottier
authors.forename: Miklos
authors.initials: M
authors.lastname: Rusvai
authors.forename: Shelly
authors.initials: S
authors.lastname: Shields
authors.forename: Eftychia
authors.initials: E
authors.lastname: Xylouri
authors.forename: Zach
authors.initials: Z
authors.lastname: Xu
authors.forename: Canio
authors.initials: C
authors.lastname: Buonavoglia
mesh_terms: D000825:Animal Structures / Q000821:virology; D000818:Animals; D016000:Cluster Analysis; D018352:Coronavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017939:Coronavirus, Canine / Q000302:isolation & purification*; D004283:Dog Diseases / Q000453:epidemiology* / Q000821:virology*; D004285:Dogs; D005060:Europe / Q000453:epidemiology; D014644:Genetic Variation; D008562:Membrane Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D015995:Prevalence; D017422:Sequence Analysis, DNA; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins