title: Isolation of microsatellite primers for Melampyrum sylvaticum (Orobanchaceae), an endangered plant in the United Kingdom.
abstract: OBJECTIVE
  Microsatellite markers were developed for the hemiparasitic plant Melampyrum sylvaticum to investigate the breeding system, genetic diversity, and structure of populations in the United Kingdom, Sweden, and Norway.
  
  
  RESULTS
  Microsatellites were isolated from genomic DNA using an enrichment protocol. Twenty-nine loci were characterized in two individuals from each of 15 geographically disparate populations ("global"). Seven polymorphic loci were further characterized in one population ("local"). The number of alleles per locus ranged from two to 12 in the global sample and one to seven in the local sample. The expected heterozygosity ranged from 0-0.75, the observed heterozygosity from 0-0.1, and the inbreeding coefficient from 0.84-1 in the local sample.
  
  
  CONCLUSIONS
  The results show the utility of these novel polymorphic microsatellite markers for further conservation genetic analyses. The strong deficit of heterozygosity across all loci in the local sample suggests the species may be inbreeding.
authors.affiliation: Royal Botanic Garden Edinburgh, 20a Inverleith Row, Edinburgh, EH3 5LR, United Kingdom. Rhiannon.Crichton@gmail.com
authors.forename: Rhiannon J
authors.initials: RJ
authors.lastname: Crichton
authors.forename: Jane
authors.initials: J
authors.lastname: Squirrell
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Woodin
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Dalrymple
authors.forename: Peter M
authors.initials: PM
authors.lastname: Hollingsworth
mesh_terms: D000483:Alleles; D017931:DNA Primers / Q000235:genetics*; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D056727:Endangered Species; D014644:Genetic Variation; D005838:Genotype; D005843:Geography; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D009664:Norway; D031670:Orobanchaceae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013548:Sweden; D006113:United Kingdom
chemical_list: D017931:DNA Primers; D018744:DNA, Plant