title: Gastric cancer differentiation using Fourier transform near-infrared spectroscopy with unsupervised pattern recognition.
abstract: The manuscript has investigated the application of near-infrared (NIR) spectroscopy for differentiation gastric cancer. The 90 spectra from cancerous and normal tissues were collected from a total of 30 surgical specimens using Fourier transform near-infrared spectroscopy (FT-NIR) equipped with a fiber-optic probe. Major spectral differences were observed in the CH-stretching second overtone (9000-7000 cm(-1)), CH-stretching first overtone (6000-5200 cm(-1)), and CH-stretching combination (4500-4000 cm(-1)) regions. By use of unsupervised pattern recognition, such as principal component analysis (PCA) and cluster analysis (CA), all spectra were classified into cancerous and normal tissue groups with accuracy up to 81.1%. The sensitivity and specificity was 100% and 68.2%, respectively. These present results indicate that CH-stretching first, combination band and second overtone regions can serve as diagnostic markers for gastric cancer.
authors.affiliation: College of Chemistry and Molecular Sciences, Wuhan University, and Gastric & Intestine Department, Hubei Cancer Hospital, Wuhan 430072, PR China.
authors.forename: Wei-song
authors.initials: WS
authors.lastname: Yi
authors.forename: Dian-sheng
authors.initials: DS
authors.lastname: Cui
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.forename: Lan-lan
authors.initials: LL
authors.lastname: Wu
authors.forename: Ai-guo
authors.initials: AG
authors.lastname: Shen
authors.forename: Ji-ming
authors.initials: JM
authors.lastname: Hu
mesh_terms: D000328:Adult; D000368:Aged; D016000:Cluster Analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D025341:Principal Component Analysis; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013270:Stomach / Q000737:chemistry / Q000473:pathology*; D013274:Stomach Neoplasms / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology