title: Analyzing PPARα/ligand interactions by chemical cross-linking and high-resolution mass spectrometry.
abstract: The combination of chemical cross-linking and high-resolution mass spectrometry is an emerging technique for monitoring conformational changes in proteins induced by drug binding. In this chapter, we describe this approach for gaining insights into the conformational changes of the peroxisome proliferator-activated receptor alpha after binding of low-molecular weight ligands. Our strategy provides a basis to efficiently characterize target protein-drug interactions.
authors.affiliation: Department of Pharmaceutical Chemistry and Bioanalytics, Institute of Pharmacy, Martin-Luther-Universität Halle-Wittenberg, Halle (Saale), Germany.
authors.forename: Mathias Q
authors.initials: MQ
authors.lastname: Müller
authors.forename: Christian H
authors.initials: CH
authors.lastname: Ihling
authors.forename: Andrea
authors.initials: A
authors.lastname: Sinz
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D008024:Ligands; D013058:Mass Spectrometry / Q000379:methods*; D008958:Models, Molecular; D008970:Molecular Weight; D036103:Nanotechnology; D047493:PPAR alpha / Q000737:chemistry* / Q000378:metabolism*; D011487:Protein Conformation / Q000187:drug effects
chemical_list: D003432:Cross-Linking Reagents; D008024:Ligands; D047493:PPAR alpha