title: Assessment of the ability of seaweed extracts to protect against hydrogen peroxide and tert-butyl hydroperoxide induced cellular damage in Caco-2 cells.
abstract: The ability of brown seaweed extracts, Ascophyllum nodosum, Laminaria hyperborea, Pelvetia canaliculata, Fucus vesiculosus and Fucus serratus to protect against tert-butyl hydroperoxide (tert-BOOH) induced stress in Caco-2 cells was investigated. Oxidative stress was determined by measuring alteration in the enzymatic activity of catalase (CAT) and superoxide dismutases (SOD) and cellular levels of glutathione (GSH). L. hyperborea, P. canaliculata and F. serratus significantly protected against tert-BOOH induced SOD reduction but did not protect against the reduction in CAT activity or the increased cellular levels of GSH. The ability of F. serratus and F. vesiculosus to protect against H(2)O(2) and tert-BOOH induced DNA damage was also assessed. The DNA protective effects of the two seaweed extracts was compared to those of three metal chelators; deferoxamine mesylate (DFO), 1,10-phenanthroline (o-phen) and 1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis (BAPTA-AM). F. serratus and F. vesiculosus significantly protected (P<0.05) against H(2)O(2) (50 μM) induced DNA damage but not tert-BOOH induced damage.
authors.affiliation: School of Food and Nutritional Sciences, University College, Cork, Ireland.
authors.forename: A M
authors.initials: AM
authors.lastname: O'Sullivan
authors.forename: Y C
authors.initials: YC
authors.lastname: O'Callaghan
authors.forename: M N
authors.initials: MN
authors.lastname: O'Grady
authors.forename: B
authors.initials: B
authors.lastname: Queguineur
authors.forename: D
authors.initials: D
authors.lastname: Hanniffy
authors.forename: D J
authors.initials: DJ
authors.lastname: Troy
authors.forename: J P
authors.initials: JP
authors.lastname: Kerry
authors.forename: N M
authors.initials: NM
authors.lastname: O'Brien
mesh_terms: D018938:Caco-2 Cells; D002374:Catalase / Q000378:metabolism; D002477:Cells / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects*; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D000459:Phaeophyceae / Q000737:chemistry*; D020011:Protective Agents / Q000494:pharmacology*; D012624:Seaweed / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism; D020122:tert-Butylhydroperoxide / Q000633:toxicity*
chemical_list: D020011:Protective Agents; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione