title: Evaluation of the asparagine synthetase level in leukemia cells by monoclonal antibodies.
abstract: L-Asparaginase (ASNase) is important for the treatment of childhood acute lymphoblastic leukemia. ASNase sensitivity has been shown to correlate with the asparagine synthetase (ASNS) protein content in acute lymphoblastic leukemia cell lines. However, there have been few studies to determine ASNS protein levels in human leukemias, since no appropriate monoclonal antibody is available for such quantitative analysis. In this study, we report the generation of anti-ASNS monoclonal antibodies, which are applicable to flow cytometry and enzyme-linked immunosorbent assay. These monoclonal antibodies should provide a valuable tool for the quantification of ASNS protein level and estimation of ASNase-resistance in leukemia cells.
authors.affiliation: Department of Molecular Biology and Medicine, The University of Tokyo, Meguro, Tokyo, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Kusano-Arai
authors.forename: Hiroko
authors.initials: H
authors.lastname: Iwanari
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Mochizuki
authors.forename: Hiromi
authors.initials: H
authors.lastname: Nakata
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Kodama
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kitoh
authors.forename: Takao
authors.initials: T
authors.lastname: Hamakubo
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D001217:Aspartate-Ammonia Ligase / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D016367:Baculoviridae / Q000235:genetics; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005822:Genetic Vectors; D006801:Humans; D007114:Immunization; D051379:Mice; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000201:enzymology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D011993:Recombinant Fusion Proteins; D001217:Aspartate-Ammonia Ligase