title: Development of a plastic-based microfluidic immunosensor chip for detection of H1N1 influenza.
abstract: Lab-on-a-chip can provide convenient and accurate diagnosis tools. In this paper, a plastic-based microfluidic immunosensor chip for the diagnosis of swine flu (H1N1) was developed by immobilizing hemagglutinin antigen on a gold surface using a genetically engineered polypeptide. A fluorescent dye-labeled antibody (Ab) was used for quantifying the concentration of Ab in the immunosensor chip using a fluorescent technique. For increasing the detection efficiency and reducing the errors, three chambers and three microchannels were designed in one microfluidic chip. This protocol could be applied to the diagnosis of other infectious diseases in a microfluidic device.
authors.affiliation: Center for Nanobio Integration & Convergence Engineering (NICE), National NanoFab Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-806, Korea. kglee@nnfc.re.kr
authors.forename: Kyoung G
authors.initials: KG
authors.lastname: Lee
authors.forename: Tae Jae
authors.initials: TJ
authors.lastname: Lee
authors.forename: Soon Woo
authors.initials: SW
authors.lastname: Jeong
authors.forename: Ho Woon
authors.initials: HW
authors.lastname: Choi
authors.forename: Nam Su
authors.initials: NS
authors.lastname: Heo
authors.forename: Jung Youn
authors.initials: JY
authors.lastname: Park
authors.forename: Tae Jung
authors.initials: TJ
authors.lastname: Park
authors.forename: Seok Jae
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D003516:Cycloparaffins / Q000737:chemistry; D004867:Equipment Design; D006046:Gold / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D053118:Influenza A Virus, H1N1 Subtype / Q000302:isolation & purification*; D007251:Influenza, Human / Q000821:virology*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D018613:Microscopy, Confocal; D010969:Plastics / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D003516:Cycloparaffins; D010969:Plastics; D006046:Gold
keywords: H1N1 influenza; immunosensor; microfluidics; plastic chip