title: Hollow fiber-based liquid-liquid-liquid micro-extraction with osmosis: II. Application to quantification of endogenous gibberellins in rice plant.
abstract: The phenomenon and benefits of osmosis in hollow fiber-based liquid-liquid-liquid micro-extraction (HF-LLLME) were theoretically discussed in part I of this study. In this work, HF-LLLME with osmosis was coupled with high performance liquid chromatography-triple quadrupole mass spectrometry (HPLC-triple quadrupole MS/MS) to analyze eight gibberellins (gibberellin A(1), gibberellin A(3), gibberellin A(4), gibberellin A(7), gibberellin A(8), gibberellin A(9), gibberellin A(19) and gibberellin A(20)) in rice plant samples. According to the theory of HF-LLLME with osmosis, single factor experiments, orthogonal design experiments and mass transfer simulation of extraction process were carried out to select the optimal conditions. Cyclohexanol - n-octanol (1:3, v/v) was selected as organic membrane. Donor phase of 12 mL was adjusted to pH 2 and 20% NaCl (w/v) was added. Acceptor phase with an initial volume of 20 μL was the solution of 0.12 mol L(-1) Na(2)CO(3)-NaHCO(3) buffer (pH 9). Temperature was chosen to be 30 °C and extraction time was selected to be 90 min. Under optimized conditions, this method provided good linearity (r, 0.99552-0.99991) and low limits of detection (0.0016-0.061 ng mL(-1)). Finally, this method was applied to the analysis of endogenous gibberellins from plant extract which was obtained with traditional solvent extraction of rice plant tissues, and the relative recoveries were from 62% to 166%.
authors.affiliation: Department of Instrumentation and Analytical Chemistry, Key Laboratory of Separation Science for Analytical Chemistry of CAS, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wu
authors.forename: Dapeng
authors.initials: D
authors.lastname: Wu
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Duan
authors.forename: Zheng
authors.initials: Z
authors.lastname: Shen
authors.forename: Yafeng
authors.initials: Y
authors.lastname: Guan
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005875:Gibberellins / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D059627:Liquid Phase Microextraction / Q000295:instrumentation*; D012275:Oryza / Q000737:chemistry*; D009995:Osmosis; D053719:Tandem Mass Spectrometry
chemical_list: D005875:Gibberellins