title: Sequence analysis of a salt tolerant metagenomic clone.
abstract: Metagenome represent an unlimited resource for discovery of novel genes. Here we report, sequence analysis of a salt tolerant metagenomic clone (6B4) from a pond water metagenomic library. Clone 6B4 had an insert of 2254 bp with G+C composition of 64.06%. DNA sequence from 6B4 showed homology to DNA sequences from pro-teobacteria indicating origin of 6B4 metagenomic insert from a yet uncharacterized proteobacteria. Two encoded proteins from clone 6B4 showed match with ATP-depen-dent Clp protease adaptor protein (ClpS) and phasin, while two truncated encoded proteins showed match with poly-3-hydroxybutyrate synthase and permease. Clp complex is known to play a role in stress tolerance. Expression of ClpS from metagenomic clone is proposed to be responsible for salt tolerance of the metagenomic clone 6B4.
authors.affiliation: Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research (CSIR), Mall Road, Delhi, 110 007 India  ; Fermentation and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, 147 002 India.
authors.forename: Raj Kishor
authors.initials: RK
authors.lastname: Kapardar
authors.forename: Ravi
authors.initials: R
authors.lastname: Ranjan
authors.forename: Munish
authors.initials: M
authors.lastname: Puri
authors.forename: Rakesh
authors.initials: R
authors.lastname: Sharma
keywords: ClpS; Metagenome; Metagenomic; Salt tolerance