title: Total ginsenosides of Radix Ginseng modulates tricarboxylic acid cycle protein expression to enhance cardiac energy metabolism in ischemic rat heart tissues.
abstract: To elucidate the underlying mechanism of cardio-protective activity of the  total ginsenosides (TGS) of Radix Ginseng, proteomic analysis using two-dimensional  gel electrophoresis (2-DE) and MALDI-TOF-TOF-MS techniques was employed for identifying the underlying targets of TGS on improvement of the energy metabolism of isolated rat heart tissues perfused in Langendorff system under ischemia-reperfusion injury conditions. The image analysis results revealed 11 differentially expressed proteins in the TGS-treated heart tissues; these proteins, including LDHB and ODP-2, were found to be closely related to the function of tricarboxylic acid (TCA) cycle that plays pivotal roles in cardiac energy metabolism. It is thus concluded that improvement of cardiac energy metabolism via activating proteins in TCA cycle could be the major action pathway and targets of TGS activity against rat heart tissue injury.
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macau, China. jrwang@must.edu.mo
authors.forename: Jing-Rong
authors.initials: JR
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Zhou
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Yi
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Jiang
authors.forename: Liang
authors.initials: L
authors.lastname: Liu
mesh_terms: D000228:Adenine Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology*; D002952:Citric Acid Cycle; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004734:Energy Metabolism / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects; D036145:Ginsenosides / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D017202:Myocardial Ischemia / Q000188:drug therapy / Q000378:metabolism*; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D005894:Panax / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D000071617:Protein Deglycase DJ-1; D020543:Proteome / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002316:Cardiotonic Agents; D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D008869:Microtubule-Associated Proteins; D009124:Muscle Proteins; D020543:Proteome; D010088:Oxidoreductases; D000228:Adenine Phosphoribosyltransferase; C404267:PARK7 protein, rat; D000071617:Protein Deglycase DJ-1