title: Quantification of urinary urate with alkylamine glass-bound uricase and arylamine glass-bound peroxidase.
abstract: Uric acid in urine was analyzed using porcine liver uricase and horseradish peroxidase immobilized on alkylamine and arylamine glass beads (pore diameter 55nm) respectively. The minimum detection limit was 5.0mg/0.1 ml urine. The recovery of added uric acid was 92%. Within and between assay CVS were <1.3% and <5.3% respectively. A good correlation (r=0.93) was found between urinary urate values obtained by a commercial kit method and the present method. The uric acid in 24hr urine of apparently healthy adults and person sufering from various diseases was found to be 450 to 900mg/24hr, 659mg/24hr (range, mean) and 910-1400mg/24hr, 1145 mg/24hr (range, mean) respectively with the present method.
authors.affiliation: Biochemistry Research Laboratory, Department of Bio-Sciences, M. D. University, 124001 Rohtak, Haryana India.
authors.forename: C S
authors.initials: CS
authors.lastname: Pundir
authors.forename: A K
authors.initials: AK
authors.lastname: Bhargava
keywords: Urate; alkylamine glass; arylamine glass; peroxidase; uricase; urine