title: PcLT, a novel C-type lectin from Procambarus clarkii, is involved in the innate defense against Vibrio alginolyticus and WSSV.
abstract: Lectins play important roles in the innate immunity. In this work, a C-type lectin, PcLT, was obtained from Procambarus clarkii which contained a carbohydrate recognition domain (CRD) with the ability to bind to Vibrio alginolyticus and white spot syndrome virus (WSSV). RT-PCR and qRT-PCR analyses demonstrated PcLT was specifically expressed in the hepatopancreas and the mRNA was markedly upregulated by V. alginolyticus and WSSV challenge, although a slight difference in timing was observed. The study also revealed upregulation of the mRNA expression and activity of immunological factors, peroxinectin, phenoloxidase, and superoxide dismutase in hemolymph in response to recombinant PcLT (rPcLT). Moreover, rPcLT also enhanced the phagocytosis, facilitated the subsequent clearance of V. alginolyticus and prolonged the survival of WSSV-infected shrimp. These results suggested that PcLT not only served as a pathogen recognition receptor (PRR), but also functioned as an immune modulator, participating in host defense against invaders.
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, PR China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Chen
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Meng
authors.forename: Jin-Ping
authors.initials: JP
authors.lastname: Xu
authors.forename: Jing-You
authors.initials: JY
authors.lastname: Yu
authors.forename: Ming-Xiang
authors.initials: MX
authors.lastname: Meng
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D003400:Astacoidea / Q000276:immunology* / Q000821:virology*; D001422:Bacterial Adhesion; D004266:DNA Virus Infections; D043143:Hepatopancreas / Q000378:metabolism*; D007113:Immunity, Innate; D007155:Immunologic Factors / Q000235:genetics / Q000378:metabolism*; D037181:Lectins, C-Type / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D009928:Organ Specificity; D010587:Phagocytosis; D051192:Receptors, Pattern Recognition / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015854:Up-Regulation; D044149:Vibrio alginolyticus / Q000276:immunology*; D053585:Virus Attachment; D046848:White spot syndrome virus 1 / Q000276:immunology*
chemical_list: D007155:Immunologic Factors; D037181:Lectins, C-Type; D051192:Receptors, Pattern Recognition; D011994:Recombinant Proteins