title: The involvement of acidic nucleoplasmic DNA-binding protein (And-1) in the regulation of prereplicative complex (pre-RC) assembly in human cells.
abstract: DNA replication in all eukaryotes starts with the process of loading the replicative helicase MCM2-7 onto chromatin during late mitosis of the cell cycle. MCM2-7 is a key component of the prereplicative complex (pre-RC), which is loaded onto chromatin by the concerted action of origin recognition complex, Cdc6, and Cdt1. Here, we demonstrate that And-1 is assembled onto chromatin in late mitosis and early G(1) phase before the assembly of pre-RC in human cells. And-1 forms complexes with MCM2-7 to facilitate the assembly of MCM2-7 onto chromatin at replication origins in late mitosis and G(1) phase. We also present data to show that depletion of And-1 significantly reduces the interaction between Cdt1 and MCM7 in G(1) phase cells. Thus, human And-1 facilitates loading of the MCM2-7 helicase onto chromatin during the assembly of pre-RC.
authors.affiliation: Department of Biochemistry and Molecular Biology, The George Washington University Medical School, Washington, D. C. 20037, USA.
authors.forename: Yongming
authors.initials: Y
authors.lastname: Li
authors.forename: Haijie
authors.initials: H
authors.lastname: Xiao
authors.forename: Christelle
authors.initials: C
authors.lastname: de Renty
authors.forename: Aimee
authors.initials: A
authors.lastname: Jaramillo-Lambert
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Han
authors.forename: Melvin L
authors.initials: ML
authors.lastname: DePamphilis
authors.forename: Kristy J
authors.initials: KJ
authors.lastname: Brown
authors.forename: Wenge
authors.initials: W
authors.lastname: Zhu
mesh_terms: D000107:Acetylation; D018797:Cell Cycle Proteins / Q000378:metabolism; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D016193:G1 Phase; D051548:Histone Acetyltransferases / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D006801:Humans; D064111:Minichromosome Maintenance Complex Component 2; D064168:Minichromosome Maintenance Complex Component 7; D009687:Nuclear Proteins / Q000378:metabolism; D011485:Protein Binding; D018741:Replication Origin; D013692:Telophase
chemical_list: C467199:CDT1 protein, human; D018797:Cell Cycle Proteins; D002843:Chromatin; D004268:DNA-Binding Proteins; D006657:Histones; D009687:Nuclear Proteins; C544202:WDHD1 protein, human; D051548:Histone Acetyltransferases; C496553:KAT7 protein, human; C084859:MCM2 protein, human; C101624:MCM7 protein, human; D064111:Minichromosome Maintenance Complex Component 2; D064168:Minichromosome Maintenance Complex Component 7