title: Plasmid-Encoded AmpC (pAmpC) in Enterobacteriaceae: epidemiology of microorganisms and resistance markers.
abstract: CMY-2 Β-lactamase is an important cause of Β-lactam resistance in Enterobacteriaceae and constitutes the most widespread pAmpC. Although CMY-2 has been previously recognized in our region, the real prevalence and epidemiology of this resistance marker was uncertain. During August-October 2009, we conducted a multicenter, prospective study to determine pAmpC prevalence and to characterize CMY-2 producing Escherichia coli associated plasmids. Plasmid-encoded AmpC prevalence was 0.9 % in enterobacteria in this period, being CMY-2 prevalent and to a lesser extent DHA. Molecular typing of CMY-2- producing Escherichia coli isolates showed several lineages. Moreover, replicon typing of cmy-2- containing plasmids displayed a broad diversity in Inc/cmy-2 links. Therefore, association of cmy-2 with specific transposon elements may be responsible for the spread of this resistance marker in Enterobacteriaceae.
authors.affiliation: Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956 (1113) Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Daniela
authors.initials: D
authors.lastname: Cejas
authors.forename: Liliana
authors.initials: L
authors.lastname: Fernández Canigia
authors.forename: Mirta
authors.initials: M
authors.lastname: Quinteros
authors.forename: Marta
authors.initials: M
authors.lastname: Giovanakis
authors.forename: Carlos
authors.initials: C
authors.lastname: Vay
authors.forename: Silvana
authors.initials: S
authors.lastname: Lascialandare
authors.forename: Daniel
authors.initials: D
authors.lastname: Mutti
authors.forename: Gastón
authors.initials: G
authors.lastname: Pagniez
authors.forename: Marisa
authors.initials: M
authors.lastname: Almuzara
authors.forename: Gabriel
authors.initials: G
authors.lastname: Gutkind
authors.forename: Marcela
authors.initials: M
authors.lastname: Radice
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use; D001118:Argentina / Q000453:epidemiology; D001426:Bacterial Proteins / Q000032:analysis*; D017931:DNA Primers; D052978:Disk Diffusion Antimicrobial Tests; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology; D006801:Humans; D010802:Phylogeny; D011446:Prospective Studies; D011815:R Factors / Q000737:chemistry*; D013045:Species Specificity; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D017931:DNA Primers; C103022:beta-lactamase CMY-2; C048795:AmpC beta-lactamases; D001618:beta-Lactamases