title: A comparative proteomics analysis of soybean leaves under biotic and abiotic treatments.
abstract: A comparative proteomic study was made to explore the molecular mechanisms, which underlie soybean root and stem defense response caused by the oomycete Phytophthora sojae strain P6497. Soybean (Glycine max cv. Xinyixiaoheidou) seedling roots were incubated in salicylic acid, methyl jasmonate, 1-amino cyclopropane-1-carboxylic acid, hydrogen peroxide, sodium nitroprusside, vitamin B(1) and P. sojae zoosperm in order to determine whether the corresponding leaves play a role in the defense response at the proteomic level. The results showed that the proteome of leaves had no significant differences. Of the 21 identified proteins identified in the study, 62 % were involved in predominately in energy functions. Those involved in protein synthesis, secondary metabolism and metabolism categories followed in abundance, where proteins involved as transporters and in transcription were the least and represented only 5 %. Those related to energy were shown to be involved in photosynthesis and photorespiration activities. The present study provides important information with regards to proteomic methods aimed to study protein regulations of the soybean-P. sojae pathosystem, especially in terms of host resistance to this pathogen.
authors.affiliation: Soybean Research Institute of Nanjing Agricultural University, National Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Jinming
authors.initials: J
authors.lastname: Zhao
authors.forename: Yumei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Bian
authors.forename: Jun
authors.initials: J
authors.lastname: Lei
authors.forename: Jutao
authors.initials: J
authors.lastname: Sun
authors.forename: Na
authors.initials: N
authors.lastname: Guo
authors.forename: Junyi
authors.initials: J
authors.lastname: Gai
authors.forename: Han
authors.initials: H
authors.lastname: Xing
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000595:Amino Acid Sequence; D003517:Cyclopentanes / Q000494:pharmacology; D054884:Host-Pathogen Interactions; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D054883:Oxylipins / Q000494:pharmacology; D010446:Peptide Fragments / Q000737:chemistry; D010838:Phytophthora / Q000502:physiology; D010935:Plant Diseases / Q000382:microbiology; D010937:Plant Growth Regulators / Q000494:pharmacology; D057865:Plant Immunity; D018515:Plant Leaves / Q000378:metabolism* / Q000382:microbiology; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D020543:Proteome / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics; D013025:Glycine max / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D013312:Stress, Physiological
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010446:Peptide Fragments; D010937:Plant Growth Regulators; D010940:Plant Proteins; D020543:Proteome; C072239:methyl jasmonate