title: Detection ofAspergillus Fumigatus based on Asp fl gene in clinical specimens.
abstract: Oligonucleotide primers were synthesised based on the gene sequence of an 18 kDa allergen/antigen ofA. fumigatus isolated from a pathogenic strain. Polymerase chain reaction (PCR) was carried out using the forward and reverse primers and genomic DNA ofA. fumigatus, A. flavus andA. niger as template. This resulted in a PCR product of 480 bp with onlyA. fumigatus. The absence of PCR product inA. flavus andA. niger with the primers of Asp fl facilitated use of these primers for detection ofA. fumigatus in clinical specimens of patients. The results were compared with microscopy, culture and serology. Application of PCR test to clinical samples of aspergillosis patients is discussed.
authors.affiliation: DNA Technologies Group, Biotechnology Division, National Institute of Standards and Technology, 20899 Gaithersburg, MD USA.
authors.forename: A
authors.initials: A
authors.lastname: Paliwal
authors.forename: N
authors.initials: N
authors.lastname: Bir
authors.forename: S
authors.initials: S
authors.lastname: Raghuraman
authors.forename: T L
authors.initials: TL
authors.lastname: Reddy
authors.forename: P U
authors.initials: PU
authors.lastname: Sarma
keywords: Aspergillus fumigatus; PCR-diagnosis; culture; microscopy