title: The polymorphisms of the MMP-1 and the MMP-3 genes and the risk of pelvic organ prolapse.
abstract: OBJECTIVE
  To investigate the associations between single nucleotide polymorphism (SNP) type 1G/2G at position -1607/-1608 of the matrix metalloproteinase (MMP)-1 gene and SNP type 5A/6A at position -1612/-1617 of the MMP-3 gene and the development of pelvic organ prolapse (POP) in women.
  
  
  METHODS
  133 patients with symptomatic POP were included in the study group. The control group consisted of 132 women with a normal pelvic floor. 1G/2G MMP-1 and 5A/6A MMP-3 SNPs were determined by polymerase chain reaction (PCR) and restriction fragments length polymorphism analysis.
  
  
  RESULTS
  When estimated individually none of the investigated SNPs were associated with POP. The combined MMP-1/MMP-3 SNP analysis showed that the following polymorphic pairs were overrepresented in women with POP: 1G/2G -5A/6A, 2G/2G -5A/6A, 2G/2G -5A/5A, 1G/1G -6A/6A, p=0.005.
  
  
  CONCLUSIONS
  The combined effect of -1607/-1608 MMP-1 and -1612/-1617 MMP-3 SNPs may contribute to the development of POP in some women.
authors.affiliation: 2nd Department of Gynecology, Medical University of Lublin, Jaczewskiego 8, 20-954, Lublin, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Skorupski
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Jankiewicz
authors.forename: Paweł
authors.initials: P
authors.lastname: Miotła
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Marczak
authors.forename: Beata
authors.initials: B
authors.lastname: Kulik-Rechberger
authors.forename: Tomasz
authors.initials: T
authors.lastname: Rechberger
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease / Q000453:epidemiology / Q000235:genetics; D005838:Genotype; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000235:genetics*; D019278:Matrix Metalloproteinase 3 / Q000235:genetics*; D008875:Middle Aged; D056887:Pelvic Organ Prolapse / Q000453:epidemiology / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors
chemical_list: D019278:Matrix Metalloproteinase 3; D020781:Matrix Metalloproteinase 1