title: Protein complexes and target genes identification by in vivo biotinylation: the STAT5 paradigm.
abstract: Signal transducers and activators of transcription (STATs) are implicated in physiological cellular processes, stress-related responses, and malignancies. This Presentation provides                      an overview on STATs, focusing on STAT5, and describes an in vivo biotinylation methodology applied in mammalian cells for identification of STAT5-containing protein complexes and                      STAT5 target genes. Our results show that in vivo biotinylation can be used efficiently to elucidate the complex STAT5-dependent transcriptional mechanisms.
authors.affiliation: Hematology/Oncology Division, Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece. ekatsantoni@bioacademy.gr
authors.forename: Eleni
authors.initials: E
authors.lastname: Katsantoni
mesh_terms: D000818:Animals; D019921:Biotinylation / Q000379:methods*; D006801:Humans; D046912:Multiprotein Complexes / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism*; D050799:STAT5 Transcription Factor / Q000378:metabolism*; D013312:Stress, Physiological; D014158:Transcription, Genetic
chemical_list: D046912:Multiprotein Complexes; D009363:Neoplasm Proteins; D050799:STAT5 Transcription Factor