title: The Igf2as transcript is exported into cytoplasm and associated with polysomes.
abstract: Murine insulin-like growth factor 2 antisense (Igf2as) transcripts originate from the opposite strand of the same Igf2 locus as the Igf2 sense mRNA. The Igf2, insulin 2 (Ins2), and H19 genes form a cluster of imprinted genes on chromosome 7. Loss of imprinting of IGF2 in humans is associated with Beckwith-Wiedemann syndrome and Silver-Russell syndrome, as well as with Wilm's tumor and colorectal cancer. We developed a RNA-FISH protocol to detect Igf2as and Igf2 transcripts. The results from the RNA-FISH were confirmed with quantitative real-time PCR and clearly indicate that the Igf2as transcripts are predominantly located in the cytoplasm of C2C12 cells. In a polysome association study, we showed that the Igf2as sedimented with polysomes in a sucrose gradient. The cellular localization of Igf2as transcripts together with polysome fractionation analysis provides compelling evidence that the Igf2as is protein coding.
authors.affiliation: Institute of Genetics, Vetsuisse Faculty, University of Bern, Bremgartenstrasse 109a, 3001, Berne, Switzerland.
authors.forename: Carolina
authors.initials: C
authors.lastname: Duart-Garcia
authors.forename: Martin H
authors.initials: MH
authors.lastname: Braunschweig
mesh_terms: D000818:Animals; D001483:Base Sequence; D001506:Beckwith-Wiedemann Syndrome / Q000235:genetics; D001692:Biological Transport; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism*; D017931:DNA Primers; D018392:Genomic Imprinting; D017404:In Situ Hybridization, Fluorescence; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D051379:Mice; D016376:Oligonucleotides, Antisense / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011132:Polyribosomes / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D056730:Silver-Russell Syndrome / Q000235:genetics
chemical_list: D017931:DNA Primers; C513121:IGF2 protein, mouse; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D007335:Insulin-Like Growth Factor II