title: Specificity of Ocimum basilicum geraniol synthase modified by its expression in different heterologous systems.
abstract: Numerous aromatic plant species produce high levels of monoterpenols, using geranyl diphosphate (GPP) as a precursor. Sweet basil (Ocimum basilicum) geraniol synthase (GES) was used to evaluate the monoterpenol profiles arising from heterologous expressions in various plant models. Grapevine (Vitis vinifera) calli were transformed using Agrobacterium tumefasciens and the plants were regenerated. Thale cress (Arabidopsis thaliana) was transformed using the floral dip method. Tobacco (Nicotiana benthamiana) leaves were agro-infiltrated for transient expression. Although, as expected, geraniol was the main product detected in the leaves, different minor products were observed in these plants (V. vinifera: citronellol and nerol; N. benthamiana: linalool and nerol; A. thaliana: none). O. basilicum GES expression was also carried out with microbial system yeasts (Saccharomyces cerevisiae) and Escherichia coli. These results suggest that the functional properties of a monoterpenol synthase depend not only on the enzyme's amino-acidic sequence, but also on the cellular background. They also suggest that some plant species or microbial expression systems could induce the simultaneous formation of several carbocations, and could thus have a natural tendency to produce a wider spectrum of monoterpenols.
authors.affiliation: Univ Strasbourg, INRA, Inst Natl Recherche Agron, Métab Second Vigne, Unit Mixte Recherche Santé Vigne & Qual Vins, 28 rue de Herrlisheim, F-68021 Colmar, France. fischer@colmar.inra.fr
authors.forename: Marc J C
authors.initials: MJ
authors.lastname: Fischer
authors.forename: Sophie
authors.initials: S
authors.lastname: Meyer
authors.forename: Patricia
authors.initials: P
authors.lastname: Claudel
authors.forename: Mireille
authors.initials: M
authors.lastname: Perrin
authors.forename: Jean François
authors.initials: JF
authors.lastname: Ginglinger
authors.forename: Claude
authors.initials: C
authors.lastname: Gertz
authors.forename: Jean E
authors.initials: JE
authors.lastname: Masson
authors.forename: Danièle
authors.initials: D
authors.lastname: Werck-Reinhardt
authors.forename: Philippe
authors.initials: P
authors.lastname: Hugueney
authors.forename: Francis
authors.initials: F
authors.lastname: Karst
mesh_terms: D060054:Agrobacterium / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D039821:Monoterpenes / Q000032:analysis* / Q000378:metabolism*; D018649:Ocimum basilicum / Q000201:enzymology* / Q000235:genetics; D010744:Phosphoric Monoester Hydrolases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D014162:Transfection / Q000379:methods*
chemical_list: D039821:Monoterpenes; D010744:Phosphoric Monoester Hydrolases