title: Development of new microsatellite markers for Salvia officinalis L. and its potential use in conservation-genetic studies of narrow endemic Salvia brachyodon Vandas.
abstract: Nine new microsatellite markers (SSR) were isolated from Salvia officinalis L. A total of 125 alleles, with 8 to 21 alleles per locus, were detected in a natural population from the east Adriatic coast. The observed heterozygosity, expected heterozygosity, and polymorphic information content ranged from 0.46 to 0.83, 0.73 to 0.93 and 0.70 to 0.92, respectively. New microsatellite markers, as well as previously published markers, were tested for cross-amplification in Salvia brachyodon Vandas, a narrow endemic species known to be present in only two localities on the Balkan Peninsula. Out of 30 microsatellite markers tested on the natural S. brachyodon population, 15 were successfully amplified. To obtain evidence of recent bottleneck events in the populations of both species, observed genetic diversity (H(E)) was compared to the expected genetic diversity at mutation-drift equilibrium (H(EQ)) and calculated from the observed number of alleles using a two-phased mutation model (TPM). Recent bottleneck events were detected only in the S. brachyodon population. This result suggests the need to reconsider the current threat category of this endemic species.
authors.affiliation: Department of Botany, Division of Biology, Faculty of Science, University of Zagreb, Marulićev trg 9A, 10000 Zagreb, Croatia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Radosavljević
authors.affiliation: Department of Seed Science and Technology, Faculty of Agriculture, University of Zagreb, Svetošimunska 25, 10000 Zagreb, Croatia.
authors.forename: Zlatko
authors.identifier: 0000-0002-9848-1601
authors.initials: Z
authors.lastname: Satovic
authors.affiliation: Centre for Plant Biotechnology and Breeding, Agronomy Department, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
authors.forename: Jernej
authors.initials: J
authors.lastname: Jakse
authors.affiliation: Centre for Plant Biotechnology and Breeding, Agronomy Department, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
authors.forename: Branka
authors.initials: B
authors.lastname: Javornik
authors.affiliation: Department of Botany, Division of Biology, Faculty of Science, University of Zagreb, Marulićev trg 9A, 10000 Zagreb, Croatia.
authors.forename: Danijela
authors.initials: D
authors.lastname: Greguraš
authors.affiliation: Institute for Adriatic Crops and Karst Reclamation, Put Duilova 11, 21000 Split, Croatia.
authors.forename: Marija
authors.initials: M
authors.lastname: Jug-Dujaković
authors.affiliation: Department of Botany, Division of Biology, Faculty of Science, University of Zagreb, Marulićev trg 9A, 10000 Zagreb, Croatia.
authors.forename: Zlatko
authors.initials: Z
authors.lastname: Liber
mesh_terms: D014644:Genetic Variation; D018895:Microsatellite Repeats; D008957:Models, Genetic; D009154:Mutation; D027543:Salvia officinalis / Q000235:genetics*
keywords: Dalmatian sage; Lamiaceae; common sage; conservation; cross-amplification; genetic bottleneck; microsatellites; population genetics; short-tooth sage