title: MiR-15a decreases bovine mammary epithelial cell viability and lactation and regulates growth hormone receptor expression.
abstract: MicroRNAs (miRNAs) are a class of small non-coding RNAs that regulate the expression of target genes at the post-transcriptional level by transcript degradation or translational inhibition. The role of bta-miR-15a in bovine mammary gland hasn’t been reported. Using miRNAs prediction software, GHR gene was predicted to be a potential target of bta-miR-15a. In this study, bovine mammary epithelial cell line was used as an in vitro cell model to address the function of bta-miR-15a on bovine mammary epithelial cells. The expression changes of bta-miR-15a and Ghr after bta-miR-15a transfection were detected by qRT-PCR; the expression of GHR protein and casein was detected by western blotting. To determine whether bta-miR-15a can affect cell viability, cells were examined using an electronic Coulter counter (CASY-TT). In conclusion, bta-miR-15a inhibited the expression of casein of bovine mammary epithelial cells, and cell number and viability were reduced by bta-miR-15a expression. Bta-miR-15a inhibited the viability of mammary epithelial cells as well as the expression of GHR mRNA and protein level, therefore suggesting that bta-miR-15a may play an important role in mammary gland physiology.
authors.affiliation: Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin 150030, Heilongjiang, China.
authors.forename: Hui-Ming
authors.initials: HM
authors.lastname: Li
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Wang
authors.forename: Qing-Zhang
authors.initials: QZ
authors.lastname: Li
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Gao
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D002364:Caseins / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D002470:Cell Survival / Q000235:genetics; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D007774:Lactation / Q000235:genetics*; D008321:Mammary Glands, Animal / Q000378:metabolism*; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D011986:Receptors, Somatotropin / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D002364:Caseins; D035683:MicroRNAs; D011986:Receptors, Somatotropin