title: Preparation of gold nanocluster bioconjugates for electron microscopy.
abstract: In this chapter, we describe types of gold nanoparticle-biomolecule conjugates and their use in electron microscopy. Included are two detailed protocols for labeling an IgG antibody with gold monolayer protected clusters. The first approach is a direct bonding approach that utilizes the ligand place exchange reaction. The second approach describes NHS-EDC coupling of Au(144)(pMBA)(60) with IgG. Also included are various characterization techniques for determining labeling efficiency.
authors.affiliation: Department of Chemistry, Colorado State University, Fort Collins, CO, USA.
authors.forename: Christine L
authors.initials: CL
authors.lastname: Heinecke
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Ackerson
mesh_terms: D001565:Benzoates / Q000737:chemistry; D002234:Carbodiimides / Q000737:chemistry; D005591:Chemical Fractionation; D020285:Cryoelectron Microscopy; D004591:Electrophoresis, Polyacrylamide Gel; D006046:Gold / Q000737:chemistry*; D007074:Immunoglobulin G / Q000378:metabolism*; D008024:Ligands; D053768:Metal Nanoparticles / Q000648:ultrastructure*; D008854:Microscopy, Electron / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: C518491:1-ethyl-3-(3-(diethylamino)propyl)carbodiimide; D001565:Benzoates; D002234:Carbodiimides; D007074:Immunoglobulin G; D008024:Ligands; D013438:Sulfhydryl Compounds; C013594:4-mercaptobenzoate; D006046:Gold