title: Cyclodextrin glucosyltransferase production by Bacillus megaterium NCR: evaluation and optimization of culture conditions using factorial design.
abstract: Statistically-based experimental designs were used to optimize the production of cyclodextrin glucosyltransferase (CGTase) from a local isolate of Bacillus megaterium using shack culture fermentation. Seven cultural conditions were examined for enzyme production and specific activity using Plackett-Burman factorial design. Fermentation time and K(2)HPO(4) level were the crucial for factors improving enzyme production process. The steepest ascent design was adopted-based on the results recorded with Plackett-Burman design. Maximal enzyme estimates (activity 56.1 U/ml, and specific activity 62.7 U/mg protein) were achieved. A verification experiment was carried out to examine model validation of this optimization.
authors.affiliation: Department of Natural and Microbial Products, National Research Center, Dokki, Cairo, Egypt.
authors.forename: E M
authors.initials: EM
authors.lastname: Ahmed
authors.forename: Heba A
authors.initials: HA
authors.lastname: El-Refai
keywords: Bacillus megaterium; Cyclodextrin glucosyltransferase; Factorial design; Optimization