title: Differentially expressed miRNAs in retinoblastoma.
abstract: MicroRNAs (miRNAs) are short non-coding RNA transcripts that have the ability to regulate the expression of target genes, and have been shown to influence the development of various tumors. The purpose of our study is to identify aberrantly expressed miRNAs in retinoblastoma for the discovery of potential therapeutic targets for this disease, and to gain a greater understanding of the mechanisms driving retinoblastoma progression. We report 41 differentially expressed miRNAs (p<0.05) in 12 retinoblastomas as compared to three normal human retinae. Of these miRNAs, many are newly identified as being differentially expressed in retinoblastoma. Further, we report the validations of five of the most downregulated miRNAs in primary human retinoblastomas (p<0.05), human retinoblastoma cell lines, and mouse retinoblastoma cell lines. This serves as the largest and most comprehensive retinoblastoma miRNA analysis to date with corresponding clinical and pathological characteristics. This is an essential step in the discovery of miRNAs associated with retinoblastoma progression, and in the identification of potential therapeutic targets for this disease.
authors.affiliation: Cancer Biology and Epigenomics Program, Children's Hospital of Chicago Research Center, Chicago, IL 60611, USA.
authors.forename: Joshua
authors.initials: J
authors.lastname: Martin
authors.forename: Paul
authors.initials: P
authors.lastname: Bryar
authors.forename: Marilyn
authors.initials: M
authors.lastname: Mets
authors.forename: Joanna
authors.initials: J
authors.lastname: Weinstein
authors.forename: Aunica
authors.initials: A
authors.lastname: Jones
authors.forename: Alissa
authors.initials: A
authors.lastname: Martin
authors.forename: Elio F
authors.initials: EF
authors.lastname: Vanin
authors.forename: Denise
authors.initials: D
authors.lastname: Scholtens
authors.forename: Fabricio F
authors.initials: FF
authors.lastname: Costa
authors.forename: Marcelo B
authors.initials: MB
authors.lastname: Soares
authors.forename: Nikia A
authors.initials: NA
authors.lastname: Laurie
mesh_terms: D000328:Adult; D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D012334:RNA, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D019572:Retinal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012175:Retinoblastoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D035683:MicroRNAs; D012334:RNA, Neoplasm