title: [Comparison of Helicobacter pylori in oral cavity and gastric mucosa according to virulence genotype (cagA and vacA m 1)].
abstract: OBJECTIVE
  To compare the virulence genotype (cagA and vacA ml genes) of Helicobacter pylori obtained simultaneously from gastric mucosa and oral cavity.
  
  
  METHODS
  Gastric samples of 18 patients were obtained by endoscopic biopsies. Oral samples of these patients were obtained from dental plaque and saliva swabs from the floor of the mouth and the base of the tongue. All samples were studied by conventional PCR and real-time PCR (RT-PCR). Virulence genes cagA and vacA ml were studied by RT- PCR.
  
  
  RESULTS
  According to presence and/or absence of cagA and vacAm1 genes, seven different combinations were observed.
  
  
  CONCLUSIONS
  These results suggest that there is a variety of genetic profiles of Helicobacter pylori in the stomach and oral cavity, with a predominance of less virulent genotypes in the patients included in this study (cagA-, vacA m1-).
authors.affiliation: Departamento de Patología y Diagnóstico, Facultad de Odontología, Universidad de Concepción, Concepción, Chile.
authors.forename: Ester
authors.initials: E
authors.lastname: Sepúlveda
authors.forename: Jessica
authors.initials: J
authors.lastname: Moreno
authors.forename: María L
authors.initials: ML
authors.lastname: Spencer
authors.forename: Sandra
authors.initials: S
authors.lastname: Quilodrán
authors.forename: Ursula
authors.initials: U
authors.lastname: Brethauer
authors.forename: Carlos
authors.initials: C
authors.lastname: Briceño
authors.forename: Apolinaria
authors.initials: A
authors.lastname: García
mesh_terms: D000942:Antigens, Bacterial / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D001706:Biopsy; D003773:Dental Plaque / Q000382:microbiology; D005753:Gastric Mucosa / Q000382:microbiology* / Q000473:pathology; D005838:Genotype; D016481:Helicobacter Infections / Q000235:genetics / Q000473:pathology*; D016480:Helicobacter pylori / Q000235:genetics / Q000472:pathogenicity; D006801:Humans; D009055:Mouth / Q000382:microbiology* / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction; D012463:Saliva / Q000382:microbiology; D014774:Virulence
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori