title: Selective cell recruitment and spatially controlled cell attachment on instructive chitosan surfaces functionalized with antibodies.
abstract: Bioactive constructs to guide cellular mobilization and function have been proposed as an approach for a new generation of biomaterials in functional tissue engineering. Adult mesenchymal stem cells have been widely used as a source for cell based therapeutic strategies, namely tissue engineering. This is a heterogeneous cell population containing many subpopulations with distinct regenerative capacity. Thus, one of the issues for the effective clinical use of stem cells in tissue engineering is the isolation of a highly purified, expandable specific subpopulation of stem cells. Antibody functionalized biomaterials could be promising candidates to isolate and recruit specific cell types. Here we propose a new concept of instructive biomaterials that are able to recruit and purify specific cell types from a mixed cell population. This biomimetic concept uses a target-specific chitosan substrate to capture specific adipose derived stem cells. Specific antibodies were covalently immobilized onto chitosan membranes using bis[sulfosuccinimidyl] suberate (BS3). Quartz crystal microbalance (QCM) was used to monitor antibody immobilization/adsorption onto the chitosan films. Specific antibodies covalently immobilized, kept their bioactivity and captured specific cell types from a mixed cell population. Microcontact printing allowed to covalently immobilize antibodies in patterns and simultaneously a spatial control in cell attachment.
authors.affiliation: 3B's Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, AvePark, Zona Industrial da Gandra, S. Cláudio do Barco Guimarães, Caldas das Taipas, 4806-909 Guimaraães, Portugal.
authors.forename: C A
authors.initials: CA
authors.lastname: Custódio
authors.forename: A M
authors.initials: AM
authors.lastname: Frias
authors.forename: A
authors.initials: A
authors.lastname: del Campo
authors.forename: R L
authors.initials: RL
authors.lastname: Reis
authors.forename: J F
authors.initials: JF
authors.lastname: Mano
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry* / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism; D062028:Bioprinting; D002448:Cell Adhesion; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods*; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D005434:Flow Cytometry; D006801:Humans; D058525:Quartz Crystal Microbalance Techniques; D015203:Reproducibility of Results
chemical_list: D055910:Antibodies, Immobilized; D015703:Antigens, CD; D048271:Chitosan