title: Peripheral blood stem-cell harvest using percutaneous arterial lines in children.
abstract: BACKGROUND
  Autologous peripheral blood stem-cell collection (PBSCC) in children has become an integral part of contemporary treatment protocols, but the procedure is often complicated due to technical issues related to vascular access. Central line placement is often implemented to surmount this problem, but is associated with complications such as bleeding, thrombosis and pneumothorax. As an alternative we have introduced the use of radial arterial lines for PBSCC in children.
  
  
  METHODS
  Data from autologous stem cell collections performed from October 2002 to December 2011 using a radial arterial line were collected.
  
  
  RESULTS
  A total of 372 PBSCC procedures were performed during the study period; an arterial line was used in 311 PBSCC's in 208 children. The average patient age and weight were 7.9 years (SD 5.4) and 28.3 kg (SD 20.4), respectively. The smallest patient was 9 months old and weighed 7 kg. The mean total volume processed was 8,593 cm(3) (SD 4,854), and the mean number of blood volumes processed was 4.3. Mean collection time for a single blood volume was 55 minutes (SD 15.5). The mean number of CD34+ cells collected per donation was 5.8 × 10(6) /kg. Ninety-seven patients (46%) required more than one collection to meet the requested CD34+ cell target. No serious adverse effects associated with vascular access occurred in this cohort.
  
  
  CONCLUSIONS
  Percutaneous placement of radial artery catheters can be rapidly and safely performed in very small infants and in children with difficult venous access. This technique provides a reliable platform for efficient PBSCC.
authors.affiliation: Bone Marrow Transplantation Unit, Schneider Children's Medical Center of Israel and the Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Ehud
authors.initials: E
authors.lastname: Even-Or
authors.forename: Anna
authors.initials: A
authors.lastname: Grunspan
authors.forename: Yakov
authors.initials: Y
authors.lastname: Swerdlow
authors.forename: Yona
authors.initials: Y
authors.lastname: Kodman
authors.forename: Anat
authors.initials: A
authors.lastname: Yahel
authors.forename: Jacob
authors.initials: J
authors.lastname: Katz
authors.forename: Aviva
authors.initials: A
authors.lastname: Krauss
authors.forename: Isaac
authors.initials: I
authors.lastname: Yaniv
authors.forename: Jerry
authors.initials: J
authors.lastname: Stein
mesh_terms: D002648:Child; D002675:Child, Preschool; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells; D006801:Humans; D007223:Infant; D008297:Male; D017534:Radial Artery; D020858:Tissue and Organ Harvesting / Q000295:instrumentation / Q000379:methods*; D014182:Transplantation, Autologous; D062666:Vascular Access Devices