title: Toward full peptide sequence coverage by dual fragmentation combining electron-transfer and higher-energy collision dissociation tandem mass spectrometry.
abstract: Increasing peptide sequence coverage by tandem mass spectrometry improves confidence in database search-based peptide identification and facilitates mapping of post-translational modifications and de novo sequencing. Inducing 2-fold fragmentation by combining electron-transfer and higher-energy collision dissociation (EThcD) generates dual fragment ion series and facilitates extensive peptide backbone fragmentation. After an initial electron-transfer dissociation step, all ions including the unreacted precursor ions are subjected to collision induced dissociation which yields b/y- and c/z-type fragment ions in a single spectrum. This new fragmentation scheme provides richer spectra and substantially increases the peptide sequence coverage and confidence in peptide identification.
authors.forename: Christian K
authors.initials: CK
authors.lastname: Frese
authors.forename: A F Maarten
authors.initials: AF
authors.lastname: Altelaar
authors.forename: Henk
authors.initials: H
authors.lastname: van den Toorn
authors.forename: Dirk
authors.initials: D
authors.lastname: Nolting
authors.forename: Jens
authors.initials: J
authors.lastname: Griep-Raming
authors.forename: Albert J R
authors.initials: AJ
authors.lastname: Heck
authors.forename: Shabaz
authors.initials: S
authors.lastname: Mohammed
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004579:Electron Transport; D006367:HeLa Cells; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013816:Thermodynamics
chemical_list: D010446:Peptide Fragments