title: Effects of EPA and DHA on lipid droplet accumulation and mRNA abundance of PAT proteins in caprine monocytes.
abstract: The present study investigated the in vitro effects on caprine monocytes of two ω-3 PUFAs, namely eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on lipid droplet formation, an emerging process of fundamental importance in innate immunity regulation. The mRNA abundance of PAT protein family (PLIN1, PLIN2 and PLIN3), involved in the formation and trafficking of the droplets, was also assessed. The effects of EPA and DHA on monocyte apoptosis were studied as well. The number of lipid droplets per cell was found to be dependent on both type and concentration of fatty acid. ω-3 PUFAs upregulated PLIN3 and PLIN2 gene expression, as well as apoptosis rate. The present findings suggest that PUFA might modify innate immune functions of goat monocytes by interfering with the formation of lipid droplets and by upregulating proteins belonging to PAT protein family.
authors.affiliation: Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria. Università di Milano, via Celoria 10, 20133 Milano, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Lecchi
authors.forename: Guido
authors.initials: G
authors.lastname: Invernizzi
authors.forename: Alessandro
authors.initials: A
authors.lastname: Agazzi
authors.forename: Silvia
authors.initials: S
authors.lastname: Modina
authors.forename: Paola
authors.initials: P
authors.lastname: Sartorelli
authors.forename: Giovanni
authors.initials: G
authors.lastname: Savoini
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Ceciliani
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053179:Caspase 7 / Q000235:genetics / Q000378:metabolism; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000502:physiology; D006041:Goats / Q000378:metabolism*; D050356:Lipid Metabolism / Q000187:drug effects; D009000:Monocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D010750:Phosphoproteins; D012333:RNA, Messenger; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid; D053148:Caspase 3; D053179:Caspase 7