title: Apoptosis of colorectal cancer UTC116 cells induced by Cantharidinate.
abstract: Effects of Cantharidinate on apoptosis of human colorectal cancer UTC-116 cells were investigated by means of 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, H and E staining, flow cytometry, and Raman Spectra analysis. The results showed Cantharidinate to exert inhibitory action on proliferation of human colorectal cancer UTC-116 cells, inducing apoptosis, arresting cells in G1 phase, with decline of S and G2 phases. In addition, the results of Raman spectrum showed significant changes in the UTC-116 cells chemical structure with stretching after the application of Cantharidinate. Taken together, these results suggest that the treatment of human colorectal cancer with Cantharidinate may be associated with multiple molecular mechanisms for apoptosis. Furthermore, similar to fluorouracil, Cantharidinate should be considered as novel assistant drug for controlling the growth of human colorectal cancer UTC-116 cells.
authors.affiliation: Department of General Surgery, Second Hospital of Jilin University, Changchun, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Hai-Cheng
authors.initials: HC
authors.lastname: Gao
authors.forename: Jing-Wei
authors.initials: JW
authors.lastname: Xu
authors.forename: Hong
authors.initials: H
authors.lastname: Cao
authors.forename: Xue-Dong
authors.initials: XD
authors.lastname: Fang
authors.forename: Hai-Mei
authors.initials: HM
authors.lastname: Gao
authors.forename: Shi-Xing
authors.initials: SX
authors.lastname: Qiao
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002193:Cantharidin / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D015179:Colorectal Neoplasms / Q000188:drug therapy / Q000473:pathology*; D005434:Flow Cytometry; D006801:Humans; D007509:Irritants / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D007509:Irritants; D002193:Cantharidin