title: Quantitative attachment and detachment of bacterial spores from fine wires through continuous and pulsed DC electrophoretic deposition.
abstract: We demonstrate the uniform attachment of bacterial spores electrophoretically onto fine wires in liquids and subsequently quantitatively detached back into suspension. It was found that the use of a pulsed voltage method resulted in a uniform coverage of spores and prevented visible bubble formation resulting from water electrolysis which tended to dislodge the spores from the wires. By monitoring the electrophoretically derived current, this method could also be used to quantitatively measure the surface charges on spores and the deposition rate. The method is generic and should be applicable to the deposition of any charged biological material (e.g., spores, bacteria, viruses) onto metal surfaces.
authors.affiliation: Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742, USA.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Zhou
authors.forename: Sarah K
authors.initials: SK
authors.lastname: Watt
authors.forename: De-Hao
authors.initials: DH
authors.lastname: Tsai
authors.forename: Vincent T
authors.initials: VT
authors.lastname: Lee
authors.forename: Michael R
authors.initials: MR
authors.lastname: Zachariah
mesh_terms: D001412:Bacillus subtilis / Q000502:physiology*; D004572:Electrolysis; D004586:Electrophoresis; D008958:Models, Molecular; D013171:Spores, Bacterial / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water