title: Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
abstract: Data on absolute molecule numbers will empower the modeling, understanding, and comparison of cellular functions and biological systems. We quantified transcriptomes and proteomes in fission yeast during cellular proliferation and quiescence. This rich resource provides the first comprehensive reference for all RNA and most protein concentrations in a eukaryote under two key physiological conditions. The integrated data set supports quantitative biology and affords unique insights into cell regulation. Although mRNAs are typically expressed in a narrow range above 1 copy/cell, most long, noncoding RNAs, except for a distinct subset, are tightly repressed below 1 copy/cell. Cell-cycle-regulated transcription tunes mRNA numbers to phase-specific requirements but can also bring about more switch-like expression. Proteins greatly exceed mRNAs in abundance and dynamic range, and concentrations are regulated to functional demands. Upon transition to quiescence, the proteome changes substantially, but, in stark contrast to mRNAs, proteins do not uniformly decrease but scale with cell volume.
authors.affiliation: University College London, Department of Genetics, Evolution and Environment and UCL Cancer Institute, London WC1E 6BT, UK.
authors.forename: Samuel
authors.initials: S
authors.lastname: Marguerat
authors.forename: Alexander
authors.initials: A
authors.lastname: Schmidt
authors.forename: Sandra
authors.initials: S
authors.lastname: Codlin
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Ruedi
authors.initials: R
authors.lastname: Aebersold
authors.forename: Jürg
authors.initials: J
authors.lastname: Bähler
mesh_terms: D002453:Cell Cycle; D013058:Mass Spectrometry / Q000379:methods; D020543:Proteome / Q000032:analysis*; D012331:RNA, Fungal / Q000032:analysis; D062085:RNA, Long Noncoding / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012568:Schizosaccharomyces / Q000737:chemistry / Q000166:cytology* / Q000235:genetics / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins / Q000032:analysis*; D017423:Sequence Analysis, RNA / Q000379:methods; D059467:Transcriptome
chemical_list: D020543:Proteome; D012331:RNA, Fungal; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D029702:Schizosaccharomyces pombe Proteins