title: Molecular cloning and characterization of a new cDNA sequence encoding a venom peptide from the centipede Scolopendra subspinipes mutilans.
abstract: Many studies have been performed on venomous peptides derived from animals. However, little of this research has focused on peptides from centipede venoms. Here, a venom gland cDNA library was successfully constructed for the centipede Scolopendra subspinipes mutilans. A new cDNA encoding the precursor of a venom peptide, named SsmTx, was cloned from the venomous gland cDNA library of the centipede S. subspinipes mutilans. The full-length SsmTx cDNA sequence is 465 nt, including a 249 nt ORF, a 45 nt 5' UTR and a 171 nt 3' UTR. There is a signal tail AATAAA 31 nt upstream of the poly (A) tail. The precursor nucleotide sequence of SsmTx encodes a signal peptide of 25 residues and a mature peptide of 57 residues, which is bridged by two pairs of disulfide bonds. SsmTx displays a unique cysteine motif that is completely different from that of other venomous animal toxins. This is the first reported cDNA sequence encoding a venom peptide from the centipede S. subspinipes mutilans.
authors.affiliation: School of Medicine, Wuhan University, Wuhan, 430071, China.
authors.forename: Wanhong
authors.initials: W
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Luo
authors.forename: Jing
authors.initials: J
authors.lastname: He
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Cao
authors.forename: Lixia
authors.initials: L
authors.lastname: Miao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001180:Arthropod Venoms / Q000235:genetics* / Q000378:metabolism; D001181:Arthropods; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D015723:Gene Library; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics* / Q000378:metabolism
chemical_list: D001180:Arthropod Venoms; D018076:DNA, Complementary; D010446:Peptide Fragments