title: The fabrication of nanosensor-based surface plasmon resonance for IgG detection.
abstract: Poly(2-hydroxyethyl methacrylate)/3-(2-imidazoline-1-yl)propyl(triethoxysilane) (PHEMA/IMEO) nanoparticles were attached on surface plasmon resonance (SPR) sensor for the real-time detection of human immunoglobulin G (IgG) in human serum. The PHEMA/IMEO nanoparticles-attached SPR sensor was characterized by Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), and contact angle measurements. IgG detection studies were performed using aqueous IgG solutions at different concentrations. In order to show the selectivity and specificity of the SPR sensor, competitive kinetic analyses were performed using IgG, albumin, hemoglobin in singular and competitive manner. Finally, IgG detection in human serum was carried out.
authors.affiliation: Department of Biology, Ağrı İbrahim Çeçen University, Ağrı, Turkey.
authors.forename: Emir Alper
authors.initials: EA
authors.lastname: Türkoğlu
authors.forename: Handan
authors.initials: H
authors.lastname: Yavuz
authors.forename: Lokman
authors.initials: L
authors.lastname: Uzun
authors.forename: Sinan
authors.initials: S
authors.lastname: Akgöl
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D000418:Albumins / Q000737:chemistry; D015374:Biosensing Techniques; D006046:Gold; D006454:Hemoglobins / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D056656:Lab-On-A-Chip Devices; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry*; D011102:Polyhydroxyethyl Methacrylate / Q000031:analogs & derivatives* / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000418:Albumins; D006454:Hemoglobins; D007074:Immunoglobulin G; D012996:Solutions; D011102:Polyhydroxyethyl Methacrylate; D006046:Gold