title: Separation and identification of thyroid autoantibodies in patients with thyroid disorders by hydrophobic column.
abstract: A method has been developed to separate and identify thyroglobulin autoantibody (TgAb) and thyroid peroxidase autoantibody (TPOAb) in serum obtained from normal and autoimmune thyroid diseases using phenyl Sepharose CL-4B hydrophobic column. The protein peaks obtained from hydrophobic column were identified as TgAb and TPOAb by comparing the elution profile of commercially purified standard thyroid autoantibodies. The similarity of the inhibitory effects of eluted proteins and of standard thyroid autoantibodies on lectin concanavalin A-RBC interaction confirmed the separation of TPO-Ab and TgAb by the hydrophobic, column. The eluted fractions from the hydrophobic column were estimated by the radio immunoassay (RIA) to confirm the presence of both auto-antibodies. This hydrophobic column method offers an advantage of visual inspection of this autoantibodies by graphic representation of peak height along with their estimation in autoimmune thyroid disorders.
authors.affiliation: Indian Institute of Chemical Biology, 4 Raja S C Mullick Road, 700032 Calcutta.
authors.forename: P S
authors.initials: PS
authors.lastname: Basu
authors.forename: R
authors.initials: R
authors.lastname: Majhi
authors.forename: S
authors.initials: S
authors.lastname: Chatterjee
authors.forename: S K
authors.initials: SK
authors.lastname: Batabyal
keywords: Autoantibodies; Hydrophobic column; Lectin; Phenylsepharose CL-4B column; Thyroid autoimmune diseases