title: Effect of Lecithin and silymarin on D-galactosamine induced toxicity in isolated hepatocytes and rats.
abstract: To investigate Lecithin for its hepatoprotective activity against D-galactosamine (D-GalN) induced toxicity in freshly isolated rat hepatocytes and animal models. Freshly isolated rat hepatocytes were exposed to Dgalactosamine (30 mM) along with/without lecithin (100 μg/ml) and the levels of selected liver enzymes were measured. Thirty six Wistar strain albino rats were used for the in vivo investigations. Lecithin 50 and 100 mg/kg.b.wt were administered for one week by oral route. Liver damage was induced by intra peritoneal administration of 400 mg/kg b.wt D-galactosamine. The antihepatotoxic effect of lecithin was observed in freshly isolated rat hepatocytes at concentration 100 μg/ml and was found to be similar to that of the standard silymarin used. Its in vivo hepatoprotective effect at 100 mg/kg b.wt was comparable with that of the standard silymarin at 100 mg/kg body weight. Lecithin was able to normalise the biochemical levels which were altered due to D-galactosamine intoxication in freshly isolated rat hepatocytes and also in animal models.
authors.affiliation: Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, 576104 Karnataka India.
authors.forename: P V
authors.initials: PV
authors.lastname: Raj
authors.forename: K
authors.initials: K
authors.lastname: Nitesh
authors.forename: H R
authors.initials: HR
authors.lastname: Chandrashekhar
authors.forename: C
authors.initials: C
authors.lastname: Mallikarjuna Rao
authors.forename: J
authors.initials: J
authors.lastname: Venkata Rao
authors.forename: N
authors.initials: N
authors.lastname: Udupa
keywords: Animal models; D-galactosamine; Hepatocytes; Hepatoprotective activity; Lecithin; Silymarin