title: Modified method for determination of serum beta-glucuronidase: A comparative study using P-nitrophenyl glucuronide and phenolphthalein glucuronide as substrate in gastrointestinal tract carcinomas.
abstract: Serum beta-glucuronidase activity was estimated by our modified method using two substrates phenolphthalein glucuronide and p-nitrophenyl glucoronide in 49 healthy subjects and 94 patients with gastrointestinal tract (GIT) carcinoma. The correlation coefficient using the two substrates was found to be r=0.8383. The method of Gabor Szasz was modified wherein the incubation time was decreased from 5 hours to 2 hours and the incubation temperature was increased from 25°C to 37°C. The increase in beta-glucuronidase activity in GIT carcinoma appears to be a good biochemical marker in patients with such type of carcinomas, a finding not reported in literature to-date.The modified quantitative method used for estimation of beta-glucuronidase is reliable, accurate, simple and rapid.
authors.affiliation: Department of Biochemistry, Tata Memorial Hospital, B/2, Gnyandeep CHS, 17th Road, Santacruz (West), 400 012 Mumbai, India.
authors.forename: M M
authors.initials: MM
authors.lastname: Khanolkar
authors.forename: A V
authors.initials: AV
authors.lastname: Sirsat
authors.forename: S S
authors.initials: SS
authors.lastname: Walvekar
authors.forename: M S
authors.initials: MS
authors.lastname: Bhansali
authors.forename: L J
authors.initials: LJ
authors.lastname: Desouza
keywords: Determination of β-glucuronidase; GIT carcinomas