title: Cytochrome P450 119 Compounds I Formed by Chemical Oxidation and Photooxidation Are the Same Species.
abstract: Compound I from cytochrome P450 119 prepared by the photooxidation method involving peroxynitrite oxidation of the resting enzyme to Compound II followed by photooxidation to Compound I was compared to Compound I generated by m-chloroperoxybenzoic acid (MCPBA) oxidation of the resting enzyme. The two methods gave the same UV/Visible spectra, the same products from oxidations of lauric acid and palmitic acid and their (ω-2,ω-2,ω-3,ω-3)-tetradeuterated analogues, and the same kinetics for oxidations of lauric acid and caprylic acid. The experimental identities between the transients produced by the two methods leave no doubt that the same Compound I species is formed by the two methods.
authors.affiliation: Department of Chemistry, University of Illinois at Chicago, 845 W. Taylor St., Chicago, IL 60617 (USA), Fax: (+1) 312-996-0431.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Su
authors.affiliation: Department of Chemistry, University of Illinois at Chicago, 845 W. Taylor St., Chicago, IL 60617 (USA), Fax: (+1) 312-996-0431.
authors.forename: John H
authors.initials: JH
authors.lastname: Horner
authors.affiliation: Department of Chemistry, University of Illinois at Chicago, 845 W. Taylor St., Chicago, IL 60617 (USA), Fax: (+1) 312-996-0431.
authors.forename: Martin
authors.initials: M
authors.lastname: Newcomb
mesh_terms: D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D007850:Lauric Acids / Q000737:chemistry; D008027:Light; D010084:Oxidation-Reduction; D019308:Palmitic Acid / Q000737:chemistry; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D013053:Spectrophotometry
chemical_list: D019843:Archaeal Proteins; D007850:Lauric Acids; D011994:Recombinant Proteins; C030358:lauric acid; D019308:Palmitic Acid; D003577:Cytochrome P-450 Enzyme System; C408874:CYP119 protein, Sulfolobus solfataricus
keywords: Compound I; cytochromes; kinetics; oxidation; spectroscopy