title: Use of specific IgE to fractions of gynandropsis gynandra pollen inin vitro diagnostic test.
abstract: The study was aimed at presence of specific IgE antibody levelsinvitro to the identified antigen. Based on positive skin test with Gynandropsis gynandra and elevated levels of total IgE (>325 IU/ml) 104 patients were selected. Healthy, asymptomatic individuals (25) with low total IgE (<325 IU/ml) were included as controls. The mean OD values by ELISA for specific IgE were 0.67±0.21, 0.57±0.18 and 0.56±0.18 with whole pollen antigen, 46-37 kD fraction and 36-32 kD fraction, respectively. The specificity and sensitivity between skin test positivity with whole pollen antigen verses fraction with mol.wt 46-37 kD was 90% and 90% and for fraction with mol.wt 36-32 kD was found to be 81.1% and 89.4%. The clusters with molecular weights 46-37 kD and 36-32 kD may be useful inin vitro diagnostic test. Fractions within these clusters need to be identified for a higher specificity.
authors.affiliation: 10/1/1, Bhagwan Mahavir Hospital and Research Center, A.C. Guards, 500 004 Hyderabad, A.P. India.
authors.forename: G Suman
authors.initials: GS
authors.lastname: Latha
authors.forename: Vijaya
authors.initials: V
authors.lastname: Lakshmi
authors.forename: H Surekha
authors.initials: HS
authors.lastname: Rani
authors.forename: K J R
authors.initials: KJ
authors.lastname: Murthy
keywords: ELISA; Gynandropsis gynandra; IgE; Sensitivity; Skin test; Specificity