title: Photoinduced fluorescence activation and nitric oxide release with biocompatible polymer nanoparticles.
abstract: A viable strategy to encapsulate a fluorophore/photochrome dyad and a nitric oxide photodonor within supramolecular assemblies of a cyclodextrin-based polymer in water was developed. The two photoresponsive guests do not interact with each other within their supramolecular container and can be operated in parallel under optical control. Specifically, the dyad permits the reversible switching of fluorescence on a microsecond timescale for hundreds of cycles, and the photodonor enables the irreversible release of nitric oxide. Furthermore, these supramolecular assemblies cross the membrane of human melanoma cancer cells and transport their cargo in the cytosol. The fluorescence of one component allows the visualization of the labeled cells, and its switchable character could, in principle, be used to acquire super-resolution images, while the release of nitric oxide from the other induces significant cell mortality. Thus, our design logic for the construction of biocompatible nanoparticles with dual functionality might evolve into the realization of valuable photoresponsive probes for imaging and therapeutic applications.
authors.affiliation: Laboratory for Molecular Photonics, Department of Chemistry, University of Miami, Coral Gables, FL 33146-0431, USA.
authors.forename: Erhan
authors.initials: E
authors.lastname: Deniz
authors.forename: Noufal
authors.initials: N
authors.lastname: Kandoth
authors.forename: Aurore
authors.initials: A
authors.lastname: Fraix
authors.forename: Venera
authors.initials: V
authors.lastname: Cardile
authors.forename: Adriana C E
authors.initials: AC
authors.lastname: Graziano
authors.forename: Debora
authors.initials: D
authors.lastname: Lo Furno
authors.forename: Ruxandra
authors.initials: R
authors.lastname: Gref
authors.forename: Françisco M
authors.initials: FM
authors.lastname: Raymo
authors.forename: Salvatore
authors.initials: S
authors.lastname: Sortino
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D045744:Cell Line, Tumor; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000633:toxicity*; D005453:Fluorescence; D006571:Heterocyclic Compounds / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D009569:Nitric Oxide / Q000737:chemistry*; D055668:Photochemical Processes; D011108:Polymers / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D006571:Heterocyclic Compounds; D011108:Polymers; D009569:Nitric Oxide