title: Detection of Bartonella spp. DNA in clinical specimens using an internally controlled real-time PCR assay.
abstract: Bartonella henselae is the causative agent of cat-scratch disease (CSD), usually presenting itself as a -self-limiting lymphadenopathy. In this chapter an internally controlled Taqman probe-based real-time PCR targeting the groEL gene of Bartonella spp. is described. This assay allows for the rapid, sensitive, and simple detection of Bartonella spp. in samples from CSD or endocarditis suspects, and it is suitable for implementation in the diagnostic microbiology laboratory.
authors.affiliation: Laboratory of Medical Microbiology, Franciscus Hospital, Roosendaal, The Netherlands. Abergmans@fzr.nl
authors.forename: Anneke M C
authors.initials: AM
authors.lastname: Bergmans
authors.forename: John W A
authors.initials: JW
authors.lastname: Rossen
mesh_terms: D000818:Animals; D001473:Bartonella / Q000235:genetics / Q000302:isolation & purification*; D002372:Cat-Scratch Disease / Q000175:diagnosis*; D002415:Cats; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D004780:Environment, Controlled; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D012015:Reference Standards; D013048:Specimen Handling / Q000379:methods
chemical_list: D004269:DNA, Bacterial