title: Effect of peptide and guest charge on the structural, mechanical and release properties of β-sheet forming peptides.
abstract: The effect of peptide charge on the self-assembly, gelation behavior, and model drug release profiles has been explored here for three octa-peptides, VEVKVEVK (VEK2), VKVKVEVK (VEK3), and VEVEVKVE (VEK1), that carry a net charge of 0, +2, and -2 at neutral pH, respectively. Transparent, self-supporting hydrogels were found to form above a critical concentration when the peptide charge modulus was >1 and this was independent of the sign of the charge. TEM, SAXS, and shear rheology revealed that there were no differences in hydrogel structure or mechanical properties when the peptides were at the same concentration and carried the same charge modulus. All peptides were found to form dense fibrillar networks formed by β-sheet rich single fibers where lateral aggregation of the fibers occurred and increased with decreasing charge modulus. Such behavior was found to correlate with an increase in hydrogel mechanical properties, demonstrating that fiber lateral aggregation is inextricably linked with the mechanical properties of these hydrogels. Two hydrophilic model drug molecules, namely napthol yellow (NY) and martius yellow (MY), were subsequently incorporated within the VEK1 and VEK3 hydrogels at pH 7 and although they did not effect the self-assembly of the peptide at a molecular level, they did effect the level of lateral fiber aggregation observed and, therefore, the mechanical properties of the hydrogels. The release of each molecule from the hydrogels was monitored over time and shown to be controlled by Fickian diffusion where the diffusion rate, D, was dependent on the ratio between the overall effective charges carried by the peptide, i.e., the fibrillar network, and the overall charges carried by the guest molecules, but independent from the hydrogel concentration and mechanical properties within the ranges investigated. This work highlights the possibility of controlling the rate of release of small drug molecules by manipulating the charges on the guest molecules as well as the charged state of the self-assembling peptide.
authors.affiliation: School of Chemical Engineering and Analytical Science & Manchester Institute of Biotechnology, The University of Manchester, Manchester, United Kingdom.
authors.forename: D
authors.initials: D
authors.lastname: Roberts
authors.forename: C
authors.initials: C
authors.lastname: Rochas
authors.forename: A
authors.initials: A
authors.lastname: Saiani
authors.forename: A F
authors.initials: AF
authors.lastname: Miller
mesh_terms: D004058:Diffusion; D004337:Drug Carriers / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D046529:Microscopy, Electron, Transmission; D009282:Naphthalenesulfonates / Q000737:chemistry; D009284:Naphthols / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D011487:Protein Conformation; D012212:Rheology; D053838:Scattering, Small Angle; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity
chemical_list: D004337:Drug Carriers; D009282:Naphthalenesulfonates; D009284:Naphthols; D010455:Peptides; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C009824:naphthol yellow; C057508:Martius yellow