title: Isolation of antithrombotic phenolic compounds from the leaves of Crataegus pinnatifida.
abstract: Four novel phenolic compounds (1-4) were isolated from the leaves of Crataegus pinnatifida, along with three known ones (5-7). Their structures were elucidated as: methyl 4-O-β-D-glucopyranosyl-3-[(2E,6E)-8-O-β-D-glucopyranosyl-3,7-dimethyl-2,6-octadienyl] benzoate (1), biphenyl-5-ol-3-O-β-D-glucoside (2), 3,4'-dimethoxy-biphenyl-5-ol-4-O-β-D-glucoside (3), (E)-6-(benzoyloxy)-1-hydroxyhex-3-en-2-O-β-D-glucoside (4), shanyenoside A (5), eriodectyol (6), and 2″-O-rhamnosyl vitexin (7), using a combination of mass spectroscopy, 1D and 2D NMR spectroscopy, and chemical analysis. The antithrombotic activity of the isolated compounds was investigated on the transgenic zebra fish system. Among them, eriodectyol (6) potently inhibited the production of thrombus.
authors.affiliation: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, PR China.
authors.forename: Shao-Jiang
authors.initials: SJ
authors.lastname: Song
authors.forename: Ling-Zhi
authors.initials: LZ
authors.lastname: Li
authors.forename: Pin-Yi
authors.initials: PY
authors.lastname: Gao
authors.forename: Yan-Qiang
authors.initials: YQ
authors.lastname: Yuan
authors.forename: Ru-Ping
authors.initials: RP
authors.lastname: Wang
authors.forename: Ke-Chun
authors.initials: KC
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D027825:Crataegus / Q000737:chemistry*; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015394:Molecular Structure; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D013927:Thrombosis / Q000517:prevention & control*; D015027:Zebrafish
chemical_list: D005343:Fibrinolytic Agents; D010636:Phenols; D010936:Plant Extracts