title: The 5' untranslated region of Apaf-1 mRNA directs translation under apoptosis conditions via a 5' end-dependent scanning mechanism.
abstract: We have previously shown that translation driven by the 5' UTR of Apaf-1 mRNA is relatively efficient in the absence of m7G-cap, but no IRES is involved. Nevertheless, it may be speculated that a "silent" IRES is activated under apoptosis conditions. Here, we show that translation of the mRNA with the Apaf-1 5' UTR is relatively resistant to apoptosis induced by etoposide when eIF4E is sequestered by 4E-BP and eIF4G is partially cleaved. However, translation under these conditions remains governed by 5' end-dependent scanning. We hypothesize that the observed phenomenon is based on the intrinsic low cap-dependence of the Apaf-1 5' UTR.
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Bldg. A, Moscow 119234, Russia.
authors.forename: Dmitri E
authors.initials: DE
authors.lastname: Andreev
authors.forename: Sergey E
authors.initials: SE
authors.lastname: Dmitriev
authors.forename: Roman
authors.initials: R
authors.lastname: Zinovkin
authors.forename: Ilya M
authors.initials: IM
authors.lastname: Terenin
authors.forename: Ivan N
authors.initials: IN
authors.lastname: Shatsky
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D053477:Apoptotic Protease-Activating Factor 1 / Q000235:genetics* / Q000378:metabolism*; D015153:Blotting, Western; D002460:Cell Line; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D020121:5' Untranslated Regions; D053477:Apoptotic Protease-Activating Factor 1; D012333:RNA, Messenger