title: The τCstF-64 polyadenylation protein controls genome expression in testis.
abstract: The τCstF-64 polyadenylation protein (gene symbol Cstf2t) is a testis-expressed orthologue of CstF-64. Mice in which Cstf2t was knocked out had a phenotype that was only detected in meiotic and postmeiotic male germ cells, giving us the opportunity to examine CstF-64 function in an isolated developmental system. We performed massively parallel clonally amplified sequencing of cDNAs from testes of wild type and Cstf2t(-/-) mice. These results revealed that loss of τCstF-64 resulted in large-scale changes in patterns of genome expression. We determined that there was a significant overrepresentation of RNAs from introns and intergenic regions in testes of Cstf2t(-/-) mice, and a concomitant use of more distal polyadenylation sites. We observed this effect particularly in intronless small genes, many of which are expressed retroposons that likely co-evolved with τCstF-64. Finally, we observed overexpression of long interspersed nuclear element (LINE) sequences in Cstf2t(-/-) testes. These results suggest that τCstF-64 plays a role in 3' end determination and transcription termination for a large range of germ cell-expressed genes.
authors.affiliation: Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark, NJ, USA.
authors.forename: Wencheng
authors.initials: W
authors.lastname: Li
authors.forename: Hsiang-Jui
authors.initials: HJ
authors.lastname: Yeh
authors.forename: Ganesh S
authors.initials: GS
authors.lastname: Shankarling
authors.forename: Zhe
authors.initials: Z
authors.lastname: Ji
authors.forename: Bin
authors.initials: B
authors.lastname: Tian
authors.forename: Clinton C
authors.initials: CC
authors.lastname: MacDonald
mesh_terms: D000818:Animals; D039241:Cleavage Stimulation Factor / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D026723:Polyadenylation / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000378:metabolism*
chemical_list: D039241:Cleavage Stimulation Factor; C492658:Cstf2t protein, mouse