title: Isolation, growth, and characterization of human renal epithelial cells using traditional and 3D methods.
abstract: The kidney is a highly heterogeneous organ that is responsible for fluid and electrolyte balance. Much interest is focused on determining the function of specific renal epithelial cells in humans, which can only be accomplished through the isolation and growth of nephron segment-specific epithelial cells. However, human renal epithelial cells are notoriously difficult to maintain in culture. This chapter describes the isolation, growth, immortalization, and characterization of the human renal proximal tubule cell. In addition, we describe new paradigms in 3D cell culture which allow the cells to maintain more in vivo-like morphology and function.
authors.affiliation: Department of Pathology, The University of Virginia, Charlottesville, VA, USA.
authors.forename: John J
authors.initials: JJ
authors.lastname: Gildea
authors.forename: Helen E
authors.initials: HE
authors.lastname: McGrath
authors.forename: Robert E
authors.initials: RE
authors.lastname: Van Sciver
authors.forename: Dora Bigler
authors.initials: DB
authors.lastname: Wang
authors.forename: Robin A
authors.initials: RA
authors.lastname: Felder
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D015925:Cryopreservation; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007687:Kidney Tubules, Proximal / Q000166:cytology*; D016086:Lentivirus / Q000235:genetics; D019098:Telomerase / Q000235:genetics; D014161:Transduction, Genetic
chemical_list: D000952:Antigens, Polyomavirus Transforming; D019098:Telomerase