title: Interchromosomal clustering of active genes at the nuclear pore complex.
abstract: Genomes are spatially organized on many levels and the positioning of genes within the nucleus contributes to their proper expression. This positioning can also result in the clustering of genes with similar expression patterns, a phenomenon sometimes called "gene kissing." We have found that yeast genes are targeted to the nuclear periphery through interaction of the nuclear pore complex with small, cis-acting "DNA zip codes" in their promoters. Our recent study demonstrated that genes with the same zip codes cluster together at the nuclear periphery. The zip codes were necessary and sufficient to induce interchromosomal clustering. Finally, we identified a transcription factor (Put3) that binds to the GRS I zip code. Put3 binds to GRS I and is required for both GRS I-dependent positioning at the nuclear periphery and interchromosomal clustering of GRS I-targeted genes. We speculate that our findings might provide insight into other types of gene kissing, some of which also require cis-acting DNA sequences and trans-acting proteins.
authors.affiliation: Department of Molecular Biosciences, Northwestern University, Evanston, IL, USA. j-brickner@northwestern.edu
authors.forename: Donna G
authors.initials: DG
authors.lastname: Brickner
authors.forename: Jason H
authors.initials: JH
authors.lastname: Brickner
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism; D016000:Cluster Analysis; D015966:Gene Expression Regulation, Fungal; D006032:Glycine-tRNA Ligase / Q000235:genetics / Q000378:metabolism; D007296:Myo-Inositol-1-Phosphate Synthase / Q000235:genetics / Q000378:metabolism; D022022:Nuclear Pore / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: C068399:PUT3 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C519641:INO1 protein, S cerevisiae; D007296:Myo-Inositol-1-Phosphate Synthase; C478769:GRS1 protein, S cerevisiae; D006032:Glycine-tRNA Ligase