title: Isolation and identification of  the  immune-relevant ribosomal protein L10 (RPL10/QM-like gene) from the large yellow croaker Pseudosciaena  crocea (Pisces: Sciaenidae).
abstract: In order to investigate the immune role of ribosomal protein L10 (RPL10/QM-like gene) in marine fish, we challenged the large yellow croaker Pseudosciaena (= Larimichthys) crocea, the most important marine fish culture species in China, by injection with a mixture of the bacteria Vibrio harveyi and V. parahaemolyticus (3:1 in volume). Microarray analysis and real-time PCR were performed 24 and 48 h post-challenge to isolate and identify the QM-like gene from the gill P. crocea (designated PcQM). The expression level of the PcQM gene did not changed significantly at 24 h post-challenge, but was significantly downregulated at 48 h post-challenge, suggesting that the gene had an immune-modulatory effect in P. crocea. Full-length PcQM cDNA and genomic sequences were obtained by rapid amplification of cDNA ends (RACE)-PCR. The sequence of the PcQM gene clustered together with those of other QM-like genes from other aquatic organisms, indicating that the QM-like gene is highly conserved in teleosts.
authors.affiliation: Department of Marine technology, College of Ocean, Nantong University, Nantong, China.
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Su
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Liu
authors.forename: S F
authors.initials: SF
authors.lastname: Niu
authors.forename: S P
authors.initials: SP
authors.lastname: Zhong
authors.forename: F
authors.initials: F
authors.lastname: Qiu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation; D016678:Genome / Q000235:genetics; D005880:Gills / Q000378:metabolism / Q000382:microbiology; D007109:Immunity / Q000235:genetics*; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010473:Perciformes / Q000235:genetics* / Q000382:microbiology; D010802:Phylogeny; D000080882:Ribosomal Protein L10; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D016415:Sequence Alignment; D014733:Vibrio / Q000502:physiology; D014735:Vibrio Infections / Q000235:genetics / Q000276:immunology
chemical_list: D018076:DNA, Complementary; D012269:Ribosomal Proteins