title: The neuroprotective effect of acute moderate alcohol consumption on caspase-3 mediated neuroapoptosis in traumatic brain injury: the role of lysosomal cathepsin L and nitric oxide.
abstract: Our aim in this study was to investigate the effect of moderate acute alcohol administration on cysteine protease mediated neuronal apoptosis and nitric oxide production in the traumatic brain injury. A total of 29 adult Sprague-Dawley male rats weighing 250-300 g were used. The rats were allocated into four groups. The first group was the control (sham-operated) group in which only a craniotomy was performed, the others were alcohol, trauma and trauma+alcohol groups. Caspase-3 enzyme activity in the trauma group increased significantly in comparison with the control group. The alcohol given group showed a decreased caspase-3 enzyme activity compared to the trauma group. The level of caspase-3 enzyme activity in the alcohol+trauma group decreased in comparison to the trauma group. SF/FEL ratio of cathepsin-L enzyme activity in the trauma group was significantly higher than in the control group. Our results indicate that moderate alcohol consumption may have protective effects on apoptotic cell death after traumatic brain injury. Protective effects of moderate ethanol consumption might be related to inhibition of lysosomal protease release and nitric oxide production.
authors.affiliation: Eskisehir Osmangazi University, The Medical School, Department of Biochemistry, Turkey.
authors.forename: Gungor
authors.initials: G
authors.lastname: Kanbak
authors.forename: Kazım
authors.initials: K
authors.lastname: Kartkaya
authors.forename: Eda
authors.initials: E
authors.lastname: Ozcelik
authors.forename: Ahmet Burak
authors.initials: AB
authors.lastname: Guvenal
authors.forename: Sibel Canbaz
authors.initials: SC
authors.lastname: Kabay
authors.forename: Gul
authors.initials: G
authors.lastname: Arslan
authors.forename: Ramazan
authors.initials: R
authors.lastname: Durmaz
mesh_terms: D000428:Alcohol Drinking; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology; D053148:Caspase 3 / Q000378:metabolism*; D056668:Cathepsin L / Q000378:metabolism*; D002492:Central Nervous System Depressants / Q000494:pharmacology*; D000431:Ethanol / Q000494:pharmacology*; D008247:Lysosomes / Q000378:metabolism*; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002492:Central Nervous System Depressants; D009419:Nerve Tissue Proteins; D009569:Nitric Oxide; D000431:Ethanol; C505410:Casp3 protein, rat; D053148:Caspase 3; D056668:Cathepsin L; C534280:Ctsl protein, rat