title: Siderophores of Stenotrophomonas maltophilia: detection and determination of their chemical nature.
abstract: Stenotrophomonas maltophilia is an emerging nosocomial pathogen. Despite the broad spectrum of syndromes associated with S. maltophilia infections, little is known about its virulence factors, including siderophore production. The aims of this work were to detect S. maltophilia siderophores and to determine their chemical nature. We studied 31 S. maltophilia isolates from device-associated infections, recovered over the period 2006-2011 at Hospital de Clínicas José de San Martín, Buenos Aires, Argentina, and the strain K279a, whose genome has been fully sequenced. The production of siderophores was screened by the chrome azurol S (CAS) agar assay, previously modified to detect siderophores in this species. When grown on modified CAS agar plates, all the clinical isolates and K279a were CAS-positive for siderophore production. In order to determine the chemical nature of siderophores, the Csáky (hydroxamate-type) and Arnow (catechol-type) assays were used. All S. maltophilia isolates produced catechol-type siderophores, but hydroxamate-type siderophores were not detected.
authors.affiliation: Cátedra de Microbiología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956 (1113) Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: García
authors.forename: Beatriz
authors.initials: B
authors.lastname: Passerini De Rossi
authors.forename: Eliana
authors.initials: E
authors.lastname: Alcaraz
authors.forename: Carlos
authors.initials: C
authors.lastname: Vay
authors.forename: Mirta
authors.initials: M
authors.lastname: Franco
mesh_terms: D015082:2,2'-Dipyridyl / Q000494:pharmacology; D001118:Argentina / Q000453:epidemiology; D016470:Bacteremia / Q000382:microbiology; D001431:Bacteriological Techniques; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D002396:Catechols / Q000302:isolation & purification*; D055499:Catheter-Related Infections / Q000382:microbiology; D003124:Colorimetry; D004396:Coloring Agents; D021821:Communicable Diseases, Emerging / Q000382:microbiology; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D007202:Indicators and Reagents; D007501:Iron / Q000032:analysis; D007502:Iron Chelating Agents / Q000494:pharmacology; D053717:Pneumonia, Ventilator-Associated / Q000382:microbiology; D017262:Siderophores / Q000302:isolation & purification*; D020615:Stenotrophomonas maltophilia / Q000737:chemistry* / Q000302:isolation & purification; D014132:Trachea / Q000382:microbiology; D014546:Urinary Catheterization / Q000009:adverse effects
chemical_list: D002396:Catechols; D004396:Coloring Agents; D007202:Indicators and Reagents; D007502:Iron Chelating Agents; D017262:Siderophores; D015082:2,2'-Dipyridyl; D007501:Iron