title: [Expression of c-MPL in leukemic stem cells from acute myeloid leukemia patients].
abstract: This study was aimed to investigate the expression of c-MPL in acute myeloid leukemia (AML) and the correlation of the c-MPL expression with CD34 and CD38, so as to define the expression of c-MPL in leukemic stem cells. The expression levels of CD34, CD38 and c-MPL were detected by flow cytometry in bone marrow cells from 29 newly diagnosed AML patients. The relationship of c-MPL positive cell ratio with clinical parameters and correlation of c-MPL with CD34 and CD38 expression in AML patients were analyzed. The results showed that expression level of c-MPL in AML patients was significantly higher than that of normal controls (P < 0.05), and the expression level of c-MPL did not correlate with age, sex, white blood cell count, AML1-ETO fusion gene and remission after chemotherapy, but the expression of c-MPL in M2 and M5 patients was higher than that of normal control (P < 0.05). Expression of c-MPL in CD34 positive AML patients was obviously higher than that in CD34 negative AML patients (P < 0.01). c-MPL was significantly higher expressed in CD34(+) cells than that in CD34(-) cells (P < 0.001), while c-MPL expression was not significantly different between CD34(+)CD38(-) and CD34(+)CD38(-) cell groups. Positive correlation between c-MPL and CD34 expression was observed (r = 0.380, P = 0.042). It is concluded that expression of c-MPL is higher in AML patients, and positively correlates with the expression level of CD34. The c-MPL expresses in leukemic stem cells.
authors.affiliation: Chinese Academy of Medical Sciences, Tianjin, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Yu
authors.forename: Shao-Wei
authors.initials: SW
authors.lastname: Qiu
authors.forename: Qing
authors.initials: Q
authors.lastname: Rao
authors.forename: Dong
authors.initials: D
authors.lastname: Lin
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Xing
authors.forename: Ke-Jing
authors.initials: KJ
authors.lastname: Tang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Tian
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Jian-Xiang
authors.initials: JX
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002648:Child; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D053628:Receptors, Thrombopoietin / Q000378:metabolism*; D055815:Young Adult
chemical_list: D053628:Receptors, Thrombopoietin; C082218:MPL protein, human