title: Comparative study on the inhibitory effects of antioxidant vitamins and radon on carbon tetrachloride-induced hepatopathy.
abstract: We have previously reported that radon inhalation activates anti-oxidative functions and inhibits carbon tetrachloride (CCl(4))-induced hepatopathy. It has also been reported that antioxidant vitamins can inhibit CCl(4)-induced hepatopathy. In the current study, we examined the comparative efficacy of treatment with radon, ascorbic acid and α-tocopherol on CCl(4)-induced hepatopathy. Mice were subjected to intraperitoneal injection of CCl(4) after inhaling approximately 1000 or 2000 Bq/m(3) radon for 24 h, or immediately after intraperitoneal injection of ascorbic acid (100, 300, or 500 mg/kg bodyweight) or α-tocopherol (100, 300, or 500 mg/kg bodyweight). We estimated the inhibitory effects on CCl(4)-induced hepatopathy based on hepatic function-associated parameters, oxidative damage-associated parameters and histological changes. The results revealed that the therapeutic effects of radon inhalation were almost equivalent to treatment with ascorbic acid at a dose of 500 mg/kg or α-tocopherol at a dose of 300 mg/kg. The activities of superoxide dismutase, catalase, and glutathione peroxidase in the liver were significantly higher in mice exposed to radon than in mice treated with CCl(4) alone. These findings suggest that radon inhalation has an anti-oxidative effect against CCl(4)-induced hepatopathy similar to the anti-oxidative effects of ascorbic acid or α-tocopherol due to the induction of anti-oxidative functions.
authors.affiliation: Graduate School of Health Sciences, Okayama University, 5-1 Shikata-cho 2-chome, Kita-ku, Okayama-shi, Okayama 700-8558, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kataoka
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Nishiyama
authors.forename: Keiko
authors.initials: K
authors.lastname: Yamato
authors.forename: Junichi
authors.initials: J
authors.lastname: Teraoka
authors.forename: Yuji
authors.initials: Y
authors.lastname: Morii
authors.forename: Akihiro
authors.initials: A
authors.lastname: Sakoda
authors.forename: Yuu
authors.initials: Y
authors.lastname: Ishimori
authors.forename: Takehito
authors.initials: T
authors.lastname: Taguchi
authors.forename: Kiyonori
authors.initials: K
authors.lastname: Yamaoka
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D001205:Ascorbic Acid / Q000008:administration & dosage; D002252:Carbon Tetrachloride Poisoning / Q000175:diagnosis / Q000378:metabolism* / Q000517:prevention & control*; D056486:Chemical and Drug Induced Liver Injury / Q000175:diagnosis / Q000378:metabolism* / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D011886:Radon / Q000008:administration & dosage*; D017382:Reactive Oxygen Species; D016896:Treatment Outcome; D014815:Vitamins / Q000008:administration & dosage*; D024502:alpha-Tocopherol / Q000008:administration & dosage
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D014815:Vitamins; D024502:alpha-Tocopherol; D001205:Ascorbic Acid; D011886:Radon