title: Determination of sertraline in rat plasma by HPLC and fluorescence detection and its application to in vivo pharmacokinetic studies.
abstract: A simple, rapid, and sensitive HPLC method based on 9H-fluoren-9-ylmethyl chloroformate derivatization for the quantification of sertraline in rat plasma has been developed, requiring a plasma sample of only 0.1 mL, which was deproteinized and derivatized for 5 min in two single steps. The obtained derivative was stable at room temperature and was determined by HPLC using a fluorescence detector. The analytical column was a C(18) column and the mobile phase was acetonitrile and water (80:20, v/v). Calibration curves were linear in the range of 10-500 ng/mL. The limit of detection was approximately 3 ng/mL, and the lower limit of quantification was established at 10 ng/mL. The bias of the method was lower than 10%, and the within day as well as between day, relative standard deviations were lower than 12%. This analytical method was successfully applied to characterize sertraline pharmacokinetics in rats following intravenous (t(1/2)  = 213 ± 48 min, Cl = 43.1 ± 8.7 mL/min, V(d)  = 11560 ± 1861 mL) and oral (C(max)  = 156 ± 76 ng/mL, t(max)  = 63.8 ± 16.3 min) administration of 2 and 5 mg, respectively.
authors.affiliation: Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Valencia, Valencia, Spain.
authors.forename: Virginia
authors.initials: V
authors.lastname: Melis
authors.forename: Iris
authors.initials: I
authors.lastname: Usach
authors.forename: José-Esteban
authors.initials: JE
authors.lastname: Peris
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D020280:Sertraline / Q000097:blood* / Q000493:pharmacokinetics; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D000928:Antidepressive Agents; D020280:Sertraline