title: Dielectric characterization of hepatocytes in suspension and embedded into two different polymeric scaffolds.
abstract: The dielectric and conductometric properties of hepatocytes in two different environments (in aqueous suspension and embedded into polymeric scaffolds) have been investigated in the frequency range from 1 kHz to 2 GHz, where the interfacial electrical polarization gives rise to marked dielectric relaxation effects. We analyzed the dielectric behavior of hepatocytes in complete medium aqueous suspensions in the light of effective medium approximation for heterogeneous systems and hepatocytes cultured into two different highly porous and interconnected polymeric structures. In the former case, we have evaluated the passive electrical parameters associated with both the plasmatic and nuclear membrane, finding a general agreement with the values reported elsewhere, based on a partially different analysis of the experimental spectra. In the latter case, we have evaluated the cell growth into two different polymeric scaffolds made of alginate and gelatin with a similar pore distribution and similar inter-connectivity. Based on a qualitative analysis of the dielectric spectra, we were able to provide evidence that alginate scaffolds allow an overall survival of cells better than gelatin scaffold can do. These indications, confirmed by biological tests on cell viability, suggest that hepatocytes embedded in alginate scaffolds are able to perform liver specific functions even over on extended period of time.
authors.affiliation: Department of Basic and Applied Biology, University of L'Aquila, L'Aquila, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Massimi
authors.forename: A
authors.initials: A
authors.lastname: Stampella
authors.forename: L Conti
authors.initials: LC
authors.lastname: Devirgiliis
authors.forename: G
authors.initials: G
authors.lastname: Rizzitelli
authors.forename: A
authors.initials: A
authors.lastname: Barbetta
authors.forename: M
authors.initials: M
authors.lastname: Dentini
authors.forename: C
authors.initials: C
authors.lastname: Cametti
mesh_terms: D058266:Dielectric Spectroscopy; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D011108:Polymers / Q000009:adverse effects / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000009:adverse effects / Q000737:chemistry*
chemical_list: D011108:Polymers