title: The multilayer nanoparticles formed by layer by layer approach for cancer-targeting therapy.
abstract: The multilayer nanoparticles (NPs) were prepared for cancer-targeting therapy using the layer by layer approach. When drug-loaded Pluronic NPs were mixed with vesicles (liposomes) in the aqueous medium, Pluronic NPs were incorporated into the vesicles to form the vesicle NPs. Then, the multilayer NPs were formed by freeze-drying the vesicle NPs in a Pluronic aqueous solution. The morphology and size distribution of the multilayer NPs were observed using a TEM and a particle size analyzer. In order to apply the multilayer NPs as a delivery system for docetaxel (DTX), which is a model anticancer drug, the release pattern of the DTX was observed and the tumor growth was monitored by injecting the multilayer NPs into the tail veins of tumor (squamous cell carcinoma)-bearing mice. The cytotoxicity of free DTX (commercial DTX formulation (Taxotere®)) and the multilayer NPs was evaluated using MTT assay. We also evaluated the tumor targeting ability of the multilayer NPs using magnetic resonance imaging. The multilayer NPs showed excellent tumor targetability and antitumor efficacy in tumor-bearing mice, caused by the enhanced permeation and retention (EPR) effect. These results suggest that the multilayer NPs could be a potential drug delivery system for cancer-targeting therapy.
authors.affiliation: College of Pharmacy, Korea University, 2511 Sejongro, Sejong 339-700, Republic of Korea.
authors.forename: Keun Sang
authors.initials: KS
authors.lastname: Oh
authors.forename: Hwanbum
authors.initials: H
authors.lastname: Lee
authors.forename: Jae Yeon
authors.initials: JY
authors.lastname: Kim
authors.forename: Eun Jin
authors.initials: EJ
authors.lastname: Koo
authors.forename: Eun Hee
authors.initials: EH
authors.lastname: Lee
authors.forename: Jae Hyung
authors.initials: JH
authors.lastname: Park
authors.forename: Sang Yoon
authors.initials: SY
authors.lastname: Kim
authors.forename: Kwangmeyung
authors.initials: K
authors.lastname: Kim
authors.forename: Ick Chan
authors.initials: IC
authors.lastname: Kwon
authors.forename: Soon Hong
authors.initials: SH
authors.lastname: Yuk
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D000077143:Docetaxel; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity; D005290:Ferric Compounds / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology; D010316:Particle Size; D020442:Poloxamer / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D043823:Taxoids / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity; D047368:Tumor Burden / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D005290:Ferric Compounds; D013501:Surface-Active Agents; D043823:Taxoids; D020442:Poloxamer; D000077143:Docetaxel; C000499:ferric oxide; D006046:Gold