title: Dietary whey protein hydrolysates increase skeletal muscle glycogen levels via activation of glycogen synthase in mice.
abstract: Previously, we have shown that consuming carbohydrate plus whey protein hydrolysates (WPHs) replenished muscle glycogen after exercise more effectively than consuming intact whey protein or branched-chain amino acids (BCAAs). The mechanism leading to superior glycogen replenishment after consuming WPH is unclear. In this 5 week intervention, ddY mice were fed experimental diets containing WPH, a mixture of whey amino acids (WAAs), or casein (control). After the intervention, gastrocnemius muscle glycogen levels were significantly higher in the WPH group (4.35 mg/g) than in the WAA (3.15 mg/g) or control (2.51 mg/g) groups. In addition, total glycogen synthase (GS) protein levels were significantly higher in the WPH group (153%) than in the WAA (89.2%) or control groups, and phosphorylated GS levels were significantly decreased in the WPH group (51.4%). These results indicate that dietary WPH may increase the muscle glycogen content through increased GS activity.
authors.affiliation: Food Science Research Laboratories, Meiji Company, Limited , 540 Naruda, Odawara, Kanagawa 250-0862, Japan. atushi.kanda.ba@meiji.com
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kanda
authors.forename: Masashi
authors.initials: M
authors.lastname: Morifuji
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Fukasawa
authors.forename: Jinichiro
authors.initials: J
authors.lastname: Koga
authors.forename: Minoru
authors.initials: M
authors.lastname: Kanegae
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kawanaka
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Higuchi
mesh_terms: D000818:Animals; D004044:Dietary Proteins / Q000378:metabolism; D004789:Enzyme Activation; D006003:Glycogen / Q000096:biosynthesis*; D006006:Glycogen Synthase / Q000378:metabolism*; D008297:Male; D051379:Mice; D008894:Milk Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D011492:Protein Hydrolysates / Q000378:metabolism*; D000067816:Whey Proteins
chemical_list: D004044:Dietary Proteins; D008894:Milk Proteins; D011492:Protein Hydrolysates; D000067816:Whey Proteins; D006003:Glycogen; D006006:Glycogen Synthase