title: Silver-doped calcium phosphate nanoparticles: synthesis, characterization, and toxic effects toward mammalian and prokaryotic cells.
abstract: Spherical silver-doped calcium phosphate nanoparticles were synthesized in a co-precipitation route from calcium nitrate/silver nitrate and ammonium phosphate in a continuous process and colloidally stabilized by carboxymethyl cellulose. Nanoparticles with 0.39 wt% silver content and a diameter of about 50-60 nm were obtained. The toxic effects toward mammalian and prokaryotic cells were determined by viability tests and determination of the minimal inhibitory and minimal bactericidal concentrations (MIC and MBC). Three mammalian cells lines, i.e. human mesenchymal stem cells (hMSC) and blood peripheral mononuclear cells (PBMC, monocytes and T-lymphocytes), and two prokaryotic strains, i.e. Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were used. Silver-doped calcium phosphate nanoparticles and silver acetate showed similar effect toward mammalian and prokaryotic cells with toxic silver concentrations in the range of 1-3 μg mL(-1).
authors.affiliation: Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, 45117 Essen, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Peetsch
authors.forename: Christina
authors.initials: C
authors.lastname: Greulich
authors.forename: Dieter
authors.initials: D
authors.lastname: Braun
authors.forename: Christian
authors.initials: C
authors.lastname: Stroetges
authors.forename: Heinz
authors.initials: H
authors.lastname: Rehage
authors.forename: Bettina
authors.initials: B
authors.lastname: Siebers
authors.forename: Manfred
authors.initials: M
authors.lastname: Köller
authors.forename: Matthias
authors.initials: M
authors.lastname: Epple
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D002130:Calcium Phosphates / Q000737:chemistry* / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D053768:Metal Nanoparticles / Q000009:adverse effects / Q000737:chemistry* / Q000648:ultrastructure; D012834:Silver / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D002130:Calcium Phosphates; D012834:Silver; C020243:calcium phosphate