title: Walleye dermal sarcoma virus: expression of a full-length clone or the rv-cyclin (orf a) gene is cytopathic to the host and human tumor cells.
abstract: Walleye dermal sarcoma virus (WDSV) is etiologically associated with a skin tumor, walleye dermal sarcoma (WDS), which develops in the fall and regresses in the spring. WDSV genome contains, in addition to gag, pol and env, three open reading frames (orfs) designated orf a (rv-cyclin), orf b and orf c. Unintegrated linear WDSV provirus DNA isolated from infected tumor cells was used to construct a full-length WDSV provirus clone pWDSV, while orf a was cloned into pSVK3 to construct the expression vector porfA. Stable co-transfection of a walleye cell line (W12) with pWDSV and pcDNA3 generated fewer and smaller G418-resistant colonies compared to the control. By Northern blot analysis, several small transcripts (2.8, 1.8, 1.2, and 0.8 kb) were detected using a WDSV LTR-specific probe. By RT-PCR and Southern blot analysis, three cDNAs (2.4, 1.6 and 0.8 kb) were identified, including both orf a and orf b messenger. Furthermore stable co-transfection of both a human lung adenocarcinoma cell line (SPC-A-1) and a cervical cancer cell line (HeLa) with pcDNA3 and ether porfA or pWDSV also generated fewer and smaller G418-resistant colonies. We conclude that expression of the full-length WDSV clone or the orf a gene inhibits the host fish and human tumor cell growth, and Orf A protein maybe a potential factor which contributes to the seasonal tumor development and regression. This is the first fish provirus clone that has been expressed in cell culture system, which will provide a new in vitro model for tumor research and oncotherapy study.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, 712100, Shaan'xi, People's Republic of China.
authors.forename: Kun
authors.initials: K
authors.lastname: Xu
authors.forename: Ting Ting
authors.initials: TT
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Cun Fang
authors.initials: CF
authors.lastname: Zhang
authors.forename: Long
authors.initials: L
authors.lastname: Zhang
authors.forename: Li Xia
authors.initials: LX
authors.lastname: Ma
authors.forename: Ying
authors.initials: Y
authors.lastname: Xin
authors.forename: Chong Hua
authors.initials: CH
authors.lastname: Ren
authors.forename: Zhi Qiang
authors.initials: ZQ
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yan
authors.forename: Daniel
authors.initials: D
authors.lastname: Martineau
authors.forename: Zhi Ying
authors.initials: ZY
authors.lastname: Zhang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016213:Cyclins / Q000235:genetics* / Q000378:metabolism; D030283:Epsilonretrovirus / Q000235:genetics* / Q000502:physiology; D005260:Female; D005393:Fish Diseases / Q000821:virology*; D015870:Gene Expression; D005814:Genes, Viral; D016679:Genome, Viral; D054884:Host-Pathogen Interactions; D006801:Humans; D008297:Male; D016366:Open Reading Frames; D011533:Proviruses / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D012878:Skin Neoplasms / Q000662:veterinary* / Q000821:virology; D014412:Tumor Virus Infections / Q000662:veterinary* / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D016213:Cyclins; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins