title: Nanoflow electrospinning serial femtosecond crystallography.
abstract: An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14-3.1 µl min(-1) to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min(-1) and diffracted to beyond 4 Å resolution, producing 14,000 indexable diffraction patterns, or four per second, from 140 µg of protein. Nanoflow electrospinning extends SFX to biological samples that necessitate minimal sample consumption.
authors.affiliation: PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
authors.forename: Raymond G
authors.initials: RG
authors.lastname: Sierra
authors.forename: Hartawan
authors.initials: H
authors.lastname: Laksmono
authors.forename: Jan
authors.initials: J
authors.lastname: Kern
authors.forename: Rosalie
authors.initials: R
authors.lastname: Tran
authors.forename: Johan
authors.initials: J
authors.lastname: Hattne
authors.forename: Roberto
authors.initials: R
authors.lastname: Alonso-Mori
authors.forename: Benedikt
authors.initials: B
authors.lastname: Lassalle-Kaiser
authors.forename: Carina
authors.initials: C
authors.lastname: Glöckner
authors.forename: Julia
authors.initials: J
authors.lastname: Hellmich
authors.forename: Donald W
authors.initials: DW
authors.lastname: Schafer
authors.forename: Nathaniel
authors.initials: N
authors.lastname: Echols
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Gildea
authors.forename: Ralf W
authors.initials: RW
authors.lastname: Grosse-Kunstleve
authors.forename: Jonas
authors.initials: J
authors.lastname: Sellberg
authors.forename: Trevor A
authors.initials: TA
authors.lastname: McQueen
authors.forename: Alan R
authors.initials: AR
authors.lastname: Fry
authors.forename: Marc M
authors.initials: MM
authors.lastname: Messerschmidt
authors.forename: Alan
authors.initials: A
authors.lastname: Miahnahri
authors.forename: M Marvin
authors.initials: MM
authors.lastname: Seibert
authors.forename: Christina Y
authors.initials: CY
authors.lastname: Hampton
authors.forename: Dmitri
authors.initials: D
authors.lastname: Starodub
authors.forename: N Duane
authors.initials: ND
authors.lastname: Loh
authors.forename: Dimosthenis
authors.initials: D
authors.lastname: Sokaras
authors.forename: Tsu-Chien
authors.initials: TC
authors.lastname: Weng
authors.forename: Petrus H
authors.initials: PH
authors.lastname: Zwart
authors.forename: Pieter
authors.initials: P
authors.lastname: Glatzel
authors.forename: Despina
authors.initials: D
authors.lastname: Milathianaki
authors.forename: William E
authors.initials: WE
authors.lastname: White
authors.forename: Paul D
authors.initials: PD
authors.lastname: Adams
authors.forename: Garth J
authors.initials: GJ
authors.lastname: Williams
authors.forename: Sébastien
authors.initials: S
authors.lastname: Boutet
authors.forename: Athina
authors.initials: A
authors.lastname: Zouni
authors.forename: Johannes
authors.initials: J
authors.lastname: Messinger
authors.forename: Nicholas K
authors.initials: NK
authors.lastname: Sauter
authors.forename: Uwe
authors.initials: U
authors.lastname: Bergmann
authors.forename: Junko
authors.initials: J
authors.lastname: Yano
authors.forename: Vittal K
authors.initials: VK
authors.lastname: Yachandra
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Bogan
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray / Q000191:economics / Q000295:instrumentation*; D004574:Electromagnetic Fields; D004867:Equipment Design; D007700:Kinetics; D007834:Lasers; D018401:Sample Size; D013820:Thermolysin / Q000737:chemistry
chemical_list: D013820:Thermolysin