title: Fluorescence recovery after photobleaching (FRAP) with a focus on F-actin.
abstract: In this unit on fluorescence recovery after photobleaching (FRAP), an imaging approach to study protein-protein interactions in situ is described. The protocols presented use confocal microscopy to examine the mobility of a fluorescent protein by measuring the recovery of the protein in a bleached area. The data gained in FRAP studies is qualitative and yields insight into relative binding affinity, binding characteristics, and the effect of treatments or mutations on protein mobility.
authors.affiliation: Department of Basic Sciences, Philadelphia College of Osteopathic Medicine, Suwanee, Georgia, USA.
authors.forename: Lori R
authors.initials: LR
authors.lastname: Hardy
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000199:Actins / Q000032:analysis* / Q000378:metabolism; D002478:Cells, Cultured; D036681:Fluorescence Recovery After Photobleaching / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000008:administration & dosage* / Q000378:metabolism; D018613:Microscopy, Confocal / Q000379:methods; D013997:Time Factors
chemical_list: D000199:Actins; D049452:Green Fluorescent Proteins