title: Cytological changes in the oral mucosa after use of a mouth rinse  with alcohol. A prospective double blind control study.
abstract: OBJECTIVE
  The aim of this preliminary study was to detect cytological changes in the oral mucosa after using a mouth wash with alcohol.
  
  
  METHODS
  A prospective double-blind, controlled study was performed, for 6 months. Group 1 consisted of 30 subjects who used a mouth rinse with 26.9% of alcohol [Listerine] and Group 2 consisted of 30 subjects who used a mouth rinse with the same ingredients but with no alcohol. We obtained three cytological samples from the oral mucosa. The presence of cytological atypia, binucleation and karyorrhesis, and type of cells were studied. We also used a fluorescent in situ hybridization technique (FISH) in 15 samples in each group, for the micronucleus.
  
  
  RESULTS
  We found no clinical mucosal alteration after using the mouth wash at the end of the study in either group. We observed no cytological differences between the groups at the end of the study (p>0.05). Regarding the study of the micronucleus by FISH, we observed no significant difference between the groups (p>0.05).
  
  
  CONCLUSIONS
  Our results showed no cytological alteration in patients using a mouth rinse with alcohol, but these findings should be considered preliminary results, to be confirmed in a greater sample of patients.
authors.affiliation: Valencia University, Service of Stomatology and Maxillofacial Surgery, University General Hospital, Valencia, Spain.
authors.forename: Jose V
authors.initials: JV
authors.lastname: Bagan
authors.forename: Francisco
authors.initials: F
authors.lastname: Vera-Sempere
authors.forename: Cristina
authors.initials: C
authors.lastname: Marzal
authors.forename: Ana
authors.initials: A
authors.lastname: Pellín-Carcelén
authors.forename: Ezequiel
authors.initials: E
authors.lastname: Martí-Bonmatí
authors.forename: Leticia
authors.initials: L
authors.lastname: Bagan
mesh_terms: D000328:Adult; D004311:Double-Blind Method; D004338:Drug Combinations; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008297:Male; D009061:Mouth Mucosa / Q000166:cytology* / Q000187:drug effects*; D009067:Mouthwashes / Q000494:pharmacology*; D011446:Prospective Studies; D012459:Salicylates / Q000494:pharmacology*; D013729:Terpenes / Q000494:pharmacology*
chemical_list: D004338:Drug Combinations; D009067:Mouthwashes; D012459:Salicylates; D013729:Terpenes; D000431:Ethanol; C057473:Listerine