title: Expression of vascular endothelial growth factor receptors VEGFR1 in cultured multiple myeloma cells: correlation with immunophenotype and drug resistance.
abstract: We studied the expression of genes encoding vascular endothelial growth factors VEGF-A, VEGF-C, VEGF-D and their receptors in cell cultures of human multiple myeloma IM9, RPMI 1640, RPMI 8226. The studied cells did not differ by the expression of growth factors. Expression of VEGFR1 receptor was detected only in IM9 cells and VEGFR2 and VEGFR3 receptors were not expressed in multiple myeloma cells. A dependence between the aberrant CD45/CD56 phenotype of human multiple myeloma cells and VEGFR1 expression in them was revealed. The only VEGFR1-positive IM9 cell culture was most resistant to Velcade (bortezomib).
authors.affiliation: Research Institute of Carcinogenesis, Moscow, Russia.
authors.forename: N N
authors.initials: NN
authors.lastname: Kalitin
authors.forename: M N
authors.initials: MN
authors.lastname: Kostyukova
authors.forename: E S
authors.initials: ES
authors.lastname: Kakpakova
authors.forename: N N
authors.initials: NN
authors.lastname: Tupitsyn
authors.forename: A F
authors.initials: AF
authors.lastname: Karamysheva
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D001897:Boronic Acids / Q000494:pharmacology; D000069286:Bortezomib; D019002:CD56 Antigen / Q000235:genetics / Q000276:immunology; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D016130:Immunophenotyping; D017493:Leukocyte Common Antigens / Q000235:genetics / Q000276:immunology; D009101:Multiple Myeloma / Q000188:drug therapy / Q000235:genetics* / Q000276:immunology; D009928:Organ Specificity; D011719:Pyrazines / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*; D042582:Vascular Endothelial Growth Factor C / Q000235:genetics*; D042643:Vascular Endothelial Growth Factor D / Q000235:genetics*; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000235:genetics*
chemical_list: D000970:Antineoplastic Agents; D001897:Boronic Acids; D019002:CD56 Antigen; D011719:Pyrazines; D012333:RNA, Messenger; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D042582:Vascular Endothelial Growth Factor C; D042643:Vascular Endothelial Growth Factor D; D000069286:Bortezomib; D040281:Vascular Endothelial Growth Factor Receptor-1; D017493:Leukocyte Common Antigens