title: A modular and noncovalent transduction system for leucine-zipper-tagged proteins.
abstract: Modes of transport: A leucine-zipper-tagged GFP was transported into cells by "zipping" it (red) to it's complementary leucine zipper (blue) functionalized with a cell-penetrating peptide (CPP). This transport system has an inherent modularity as the CPP is "clicked" to the leucine zipper, and then noncovalently bound to the protein, thus making it system particularly useful for targeting studies.
authors.affiliation: Radboud University Nijmegen, Institute for Molecules and Materials, Department of Organic Chemistry, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.
authors.forename: Morten B
authors.initials: MB
authors.lastname: Hansen
authors.forename: Wouter P R
authors.initials: WP
authors.lastname: Verdurmen
authors.forename: Elizabeth H P
authors.initials: EH
authors.lastname: Leunissen
authors.forename: Inge
authors.initials: I
authors.lastname: Minten
authors.forename: Jan C M
authors.initials: JC
authors.lastname: van Hest
authors.forename: Roland
authors.initials: R
authors.lastname: Brock
authors.forename: Dennis W P M
authors.initials: DW
authors.lastname: Löwik
mesh_terms: D000595:Amino Acid Sequence; D001692:Biological Transport; D057846:Cell-Penetrating Peptides / Q000737:chemistry / Q000378:metabolism*; D015696:Gene Products, tat / Q000737:chemistry / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D016350:Leucine Zippers; D008958:Models, Molecular; D008969:Molecular Sequence Data
chemical_list: D057846:Cell-Penetrating Peptides; D015696:Gene Products, tat; D049452:Green Fluorescent Proteins