title: Effects of high tidal volume mechanical ventilation on production of cytokines, iNOS, and MIP-1β proteins in pigs.
abstract: The aim of this study was to investigate longitudinal changes of the pulmonary inflammatory process as a result of mechanical stress due to mechanical ventilation. The concentrations of IL-8, TNF-α, MIP-1β, nitrites/nitrates, and inducible nitric oxide synthases (iNOS) were investigated indicate in bronchoalveolar lavage (BAL). Twenty-three piglets were divided into three groups. Group I: animals breathing spontaneously; group II: mechanical ventilation (tidal volume (TV) = 7 mL/kg, PEEP = 5 cmH(2)O); group III: mechanical ventilation (TV = 15 mL/kg, PEEP = 0 cmH(2)0). Concentrations of BAL nitrites/nitrates from groups II and III increased during the first hour of mechanical ventilation (P = .03 and .02, respectively). The highest expression of iNOS was observed during the first hour in groups II and III. IL-8 concentration increased significantly in groups II and III. Production of TNF-α increased significantly in group III during the second and third hour (P = .01). Concentration of MIP-1β was significantly increased in groups II and III after the first hour (P = .012 and P = .008, respectively).
authors.affiliation: Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University, Prague, Czech Republic. vaclav.vobruba@vfn.cz
authors.forename: Václav
authors.initials: V
authors.lastname: Vobruba
authors.forename: Oxana V
authors.initials: OV
authors.lastname: Klimenko
authors.forename: Jiri
authors.initials: J
authors.lastname: Kobr
authors.forename: Olga
authors.initials: O
authors.lastname: Cerna
authors.forename: Pavla
authors.initials: P
authors.lastname: Pokorna
authors.forename: Ivan
authors.initials: I
authors.lastname: Mikula
authors.forename: Jan
authors.initials: J
authors.lastname: Hridel
authors.forename: Olga
authors.initials: O
authors.lastname: Brantova
authors.forename: Pavel
authors.initials: P
authors.lastname: Martasek
mesh_terms: D055371:Acute Lung Injury / Q000209:etiology / Q000378:metabolism*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D054407:Chemokine CCL4 / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism; D008168:Lung / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D008170:Lung Compliance / Q000502:physiology; D009566:Nitrates / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism; D011175:Positive-Pressure Respiration / Q000295:instrumentation; D011650:Pulmonary Alveoli / Q000378:metabolism / Q000473:pathology; D012121:Respiration, Artificial / Q000009:adverse effects*; D013552:Swine; D013990:Tidal Volume; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D054407:Chemokine CCL4; D016207:Cytokines; D016209:Interleukin-8; D009566:Nitrates; D009573:Nitrites; D014409:Tumor Necrosis Factor-alpha; D052247:Nitric Oxide Synthase Type II