title: The analytical application and spectral investigation of DNA-CPB-emodin and sensitive determination of DNA by resonance Rayleigh light scattering technique.
abstract: A new sensitive DNA probe containing cetylpyridinium bromide (CPB) and emodin (an effective component of Chinese herbal medicine) was developed using the resonance Rayleigh light scattering (RLS) technique. A novel assay was first developed to detect DNA at nanogram level based on the ternary system of DNA-CPB-emodin. The RLS signal of DNA was enhanced remarkably in the presence of emodin-CPB, and the enhanced RLS intensity at 340.0 nm was in direct proportion to DNA concentration in the range of 0.01-2.72 μg mL(-1) with a good linear relationship. The detection limit was 1.5 ng mL(-1). Three synthetic DNA samples were measured obtaining satisfactory results, the recovery was 97.6-107.3%.
authors.affiliation: College of Chemistry, Changchun Normal University, Changchun 130032, PR China. sy_bi@sina.com
authors.forename: Shuyun
authors.initials: S
authors.lastname: Bi
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Tianjiao
authors.initials: T
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Pang
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhao
mesh_terms: D001965:Bromides / Q000737:chemistry; D002594:Cetylpyridinium / Q000737:chemistry; D004247:DNA / Q000032:analysis*; D015342:DNA Probes / Q000737:chemistry; D004642:Emodin / Q000737:chemistry; D008027:Light; D057230:Limit of Detection; D012542:Scattering, Radiation
chemical_list: D001965:Bromides; D015342:DNA Probes; D004247:DNA; D002594:Cetylpyridinium; D004642:Emodin