title: The genome sequence of an isolate of Indian citrus ringspot virus infecting the sweet orange in India.
abstract: Whole-genome sequencing of an isolate of Mandarivirus infecting the sweet orange [Citrus sinensis (L) Blanco] in the western part of India (Pune) was done. The single-stranded positive-sense RNA genome of Indian citrus ringspot virus (ICRSV) Pune has 7,560 nucleotides (nt), excluding a poly(A) tail, comprised of 27.98% (2,115 nt) A, 32.12% (2,428 nt) C, 19.68% (1,488 nt) G, and 20.22% (1,529 nt) T residues. The genome, organized into six open reading frames (ORFs), shares 97.7% sequence identity with the complete genome of the ICRSV K1 isolate (AF406744.1) infecting the kinnow (Citrus reticulate Blanco, a hybrid between King and Willow mandarins) in north India. The ICRSV Pune genome formed a complex secondary structure with a large number of unpaired cytosine-rich regions, and recombination analysis highlighted potential recombination in the ICRSV genome.
authors.affiliation: Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, India.
authors.forename: Prabha
authors.initials: P
authors.lastname: K
authors.forename: V K
authors.initials: VK
authors.lastname: Baranwal
mesh_terms: D032084:Citrus sinensis / Q000821:virology*; D052677:Flexiviridae / Q000235:genetics*; D016679:Genome, Viral; D007194:India; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral