title: Contribution of REN gene MBbo I polymorphism in conferring risk for essential hypertension: a case control study from South India.
abstract: BACKGROUND
  Renin is a rate-limiting enzyme of the renin-angiotensin-aldosterone system (RAAS) that plays a crucial role in the regulation of blood pressure. The renin gene has been suggested as a marker for genetic predisposition to essential hypertension (EHT) in humans. The purpose of the study is to explore the association of a genetic marker of renin gene Mbo I polymorphism with EHT in the South Indian population.
  
  
  METHODS
  A total of 279 hypertensive and 200 normotensive subjects were genotyped for REN Mbo I polymorphism (RFLP) using the PCR-restriction fragment length polymorphism method.
  
  
  RESULTS
  There were no significant differences in the distribution of genotypes and alleles for REN gene Mbo I polymorphism between hypertensive cases and controls (p>0.05). The genotypic and allele frequencies were in Hardy-Weinberg equilibrium both in cases and controls. Females with Mbo I AA+GA genotypes had 1.87-fold higher risk to develop hypertension as compared with those with GG genotype (odds ratio 1.87; 95% confidence interval = 0.98-3.56, p = 0.057).
  
  
  CONCLUSIONS
  Our results indicate that renin gene Mbo I site polymorphism is not associated with blood pressure levels and risk of hypertension. However, the present study demonstrates risk for females who are carriers of REN Mbo I A allele for developing essential hypertension.
authors.affiliation: Department of Genetics, Osmania University, Hyderabad, India.
authors.forename: U
authors.initials: U
authors.lastname: Mohana Vamsi
authors.forename: N
authors.initials: N
authors.lastname: Swapna
authors.forename: G
authors.initials: G
authors.lastname: Usha
authors.forename: S
authors.initials: S
authors.lastname: Vishnupriya
authors.forename: T
authors.initials: T
authors.lastname: Padma
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D003710:Demography; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics*; D000075222:Essential Hypertension; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D007194:India; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012083:Renin / Q000235:genetics*; D012307:Risk Factors; D012727:Sex Characteristics
chemical_list: D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases; D012083:Renin
keywords: Essential hypertension; Mbo l polymorphism; PCR-RFLP; renin