title: Actinoplanes hulinensis sp. nov., a novel actinomycete isolated from soybean root (Glycine max (L.) Merr).
abstract: A novel actinomycete, designated strain NEAU-M9(T), was isolated from soybean root (Glycine max (L.) Merr) and characterized using a polyphasic approach. 16S rRNA gene sequence similarity studies showed that strain NEAU-M9(T) belonged to the genus Actinoplanes, being most closely related to Actinoplanes campanulatus DSM 43148(T) (98.85 %), Actinoplanes capillaceus DSM 44859(T) (98.70 %), Actinoplanes lobatus DSM 43150(T) (98.30 %), Actinoplanes auranticolor DSM 43031(T) (98.23 %) and Actinoplanes sichuanensis 03-723(T) (98.06 %); similarity to other type strains of the genus Actinoplanes ranged from 95.87 to 97.56 %. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that the isolate formed a distinct phyletic line with A. campanulatus DSM 43148(T) and A. capillaceus DSM 44859(T). This branching pattern was also supported by the tree constructed with the maximum-likelihood method. However, the low level of DNA-DNA relatedness allowed the isolate to be differentiated from the above-mentioned two Actinoplanes species. Moreover, strain NEAU-M9(T) could also be distinguished from the most closely related species by morphological, physiological and characteristics. Therefore, it is proposed that strain NEAU-M9(T) represents a novel Actinoplanes species, Actinoplanes hulinensis sp. nov. The type strain of Actinoplanes hulinensis is NEAU-M9(T) (= CGMCC 4.7036(T) = DSM 45728(T)).
authors.affiliation: College of Agriculture, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Shen
authors.forename: Chongxi
authors.initials: C
authors.lastname: Liu
authors.forename: Xiangjing
authors.initials: X
authors.lastname: Wang
authors.forename: Junwei
authors.initials: J
authors.lastname: Zhao
authors.forename: Feiyu
authors.initials: F
authors.lastname: Jia
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Deguang
authors.initials: D
authors.lastname: Yang
authors.forename: Wensheng
authors.initials: W
authors.lastname: Xiang
mesh_terms: D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008848:Micromonosporaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013025:Glycine max / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S