title: Biolistic transformation of Caenorhabditis elegans.
abstract: The ability to generate transgenic animals to study gene expression and function is a powerful and important part of the Caenorhabditis elegans genetic toolbox. Transgenic animals can be created by introducing exogenous DNA into the worm germline either by microinjection or by microparticle bombardment (biolistic transformation). In this chapter we describe a simple and robust protocol to generate transgenic C. elegans animals by biolistic transformation with gold particles using the Bio-Rad PDS-1000/He system with Hepta adapter and unc-119 selection marker. We also point out the steps that need special attention to achieve successful transformations.
authors.affiliation: Hubrecht Institute and University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Meltem
authors.initials: M
authors.lastname: Isik
authors.forename: Eugene
authors.initials: E
authors.lastname: Berezikov
mesh_terms: D000818:Animals; D019470:Biolistics / Q000295:instrumentation*; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000254:growth & development; D003470:Culture Media / Q000737:chemistry; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics; D004531:Eggs; D006046:Gold / Q000737:chemistry; D014170:Transformation, Genetic
chemical_list: D003470:Culture Media; D006046:Gold; D004247:DNA