title: Correlation of urine TMPRSS2:ERG and PCA3 to ERG+ and total prostate cancer burden.
abstract: ERG rearrangements (most commonly transmembrane protease, serine 2 [TMPRSS2]:ERG [T2:ERG] gene fusions) have been identified in approximately 50% of prostate cancers . Quantification of T2:ERG in postdigital rectal examination urine, in combination with PCA3, improves the performance of serum prostate-specific antigen for prostate cancer prediction on biopsy. Here we compared urine T2:ERG and PCA3 scores with ERG+ (determined with immunohistochemical analysis) and total prostate cancer burden in 41 mapped prostatectomies. Prostatectomies had a median of 3 tumor foci (range, 1-15) and 2.6 cm of summed linear tumor dimension (range, 0.6-7.1 cm). Urine T2:ERG score correlated most with summed linear ERG+ tumor dimension and number of ERG+ foci (r(s) = 0.68 and 0.67, respectively, both P < .001). Urine PCA3 score showed weaker correlation with both number of tumor foci (r(s) = 0.34, P = .03) and summed linear tumor dimension (r(s) = 0.26, P = .10). In summary, we demonstrate a strong correlation between urine T2:ERG score and total ERG+ prostate cancer burden at prostatectomy, consistent with high tumor specificity.
authors.affiliation: Dept of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
authors.forename: Allison
authors.initials: A
authors.lastname: Young
authors.forename: Nallasivam
authors.initials: N
authors.lastname: Palanisamy
authors.forename: Javed
authors.initials: J
authors.lastname: Siddiqui
authors.forename: David P
authors.initials: DP
authors.lastname: Wood
authors.forename: John T
authors.initials: JT
authors.lastname: Wei
authors.forename: Arul M
authors.initials: AM
authors.lastname: Chinnaiyan
authors.forename: Lakshmi P
authors.initials: LP
authors.lastname: Kunju
authors.forename: Scott A
authors.initials: SA
authors.lastname: Tomlins
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000652:urine*; D006801:Humans; D008297:Male; D060787:Neoplasm Grading; D050596:Oncogene Fusion; D015514:Oncogene Proteins, Fusion / Q000652:urine; D011467:Prostate / Q000473:pathology* / Q000601:surgery; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000473:pathology / Q000601:surgery / Q000652:urine*; D012697:Serine Endopeptidases / Q000652:urine*; D015534:Trans-Activators / Q000652:urine*; D000071230:Transcriptional Regulator ERG
chemical_list: D014408:Biomarkers, Tumor; C072312:ERG protein, human; D015514:Oncogene Proteins, Fusion; D015534:Trans-Activators; D000071230:Transcriptional Regulator ERG; D012697:Serine Endopeptidases; C421305:TMPRSS2 protein, human