title: Purification and characterization of a novel lectin with antiphytovirus activities from the wild mushroom Paxillus involutus.
abstract: A novel lectin was isolated from the dried fruiting bodies of the wild mushroom Paxillus involutus. Isolation was conducted by anion exchange chromatography on DEAE-Cellulose, Q-Sepharose and gel filtration on Superdex 75 using a fast protein liquid chromatography (FPLC) system. This lectin had a molecular mass of 28 kDa and was composed of four identical subunits, each with a molecular mass of 7 kDa. N-terminal amino acid sequence of the P. involutus lectin was determined to be CTCAVFLNNTTVKS, which showed a low level of similarity to mushroom lectin sequences reported previously. The biochemical properties of this lectin were determined, and the hemagglutinating activity was inhibited by inulin and O-Nitrophenyl-β-D-galacto-pyranoside. Additionally, Ca2+, Zn2+, Cd2+, Fe2+, and Al3+ inhibited its hemagglutinating activity, while Cu2+ promoted this activity. This lectin exhibited poor thermostability and was sensitive to HCl, but it had a high tolerance to NaOH exposure. In terms of biological properties, this lectin manifested antiphytovirus activity towards tobacco mosaic virus (TMV) with a 70.61% inhibition at a concentration of 200 µg/mL. This lectin was devoid of inhibitory activities toward pathogenic fungi and HIV-1 reverse transcriptase, and antiproliferative activities were observed in tumor cell lines including lung cancer A-549 and human colon cancer HCT-8 cells.
authors.affiliation: Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
authors.forename: Shou Xian
authors.initials: SX
authors.lastname: Wang
authors.forename: Guo Qing
authors.initials: GQ
authors.lastname: Zhang
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhao
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xiao
authors.initials: X
authors.lastname: Li Geng
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: He Xiang
authors.initials: HX
authors.lastname: Wang
mesh_terms: D000364:Agaricus / Q000737:chemistry*; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002241:Carbohydrates / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D037102:Lectins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D008670:Metals / Q000737:chemistry; D055550:Protein Stability; D014027:Tobacco Mosaic Virus / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D002241:Carbohydrates; D005656:Fungal Proteins; D037102:Lectins; D008670:Metals