title: Statistical optimization of alkaline xylanase production from Streptomyces violaceoruber under submerged fermentation using response surface methodology.
abstract: Response surface methodology employing central composite design (CCD) was used to optimize fermentation medium for the production of cellulase-free, alkaline xylanase from Streptomyces violaceoruber under submerged fermentation. The design was employed by selecting wheat bran, peptone, beef extract, incubation time and agitation as model factors. A second-order quadratic model and response surface method showed that the optimum conditions for xylanase production (wheat bran 3.5 % (w/v), peptone 0.8 % (w/v), beef extract 0.8 % (w/v), incubation time 36 h and agitation 250 rpm) results in 3.0-fold improvement in alkaline xylanase production (1500.0 IUml(-1)) as compared to initial level (500.0 IUml(-1)) after 36 h of fermentation, whereas its value predicted by the quadratic model was 1347 IUml(-1). Analysis of variance (ANOVA) showed a high coefficient of determination (R(2)) value of 0.9718, ensuring a satisfactory adjustment of the quadratic model with the experimental data.The economical and cellulase-free nature of xylanase would enhance its applicability in pulp and paper industry.
authors.affiliation: Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110 021 India.
authors.forename: S
authors.initials: S
authors.lastname: Khurana
authors.forename: M
authors.initials: M
authors.lastname: Kapoor
authors.forename: S
authors.initials: S
authors.lastname: Gupta
authors.forename: R C
authors.initials: RC
authors.lastname: Kuhad
keywords: Alkaline; CCD; RSM; Streptomyces violaceoruber; Xylanase