title: Evidence for chemoreceptors with bimodular ligand-binding regions harboring two signal-binding sites.
abstract: Chemoreceptor-based signaling is a central mechanism in bacterial signal transduction. Receptors are classified according to the size of their ligand-binding region. The well-studied cluster I proteins have a 100- to 150-residue ligand-binding region that contains a single site for chemoattractant recognition. Cluster II receptors, which contain a 220- to 300-residue ligand-binding region and which are almost as abundant as cluster I receptors, remain largely uncharacterized. Here, we report high-resolution structures of the ligand-binding region of the cluster II McpS chemotaxis receptor (McpS-LBR) of Pseudomonas putida KT2440 in complex with different chemoattractants. The structure of McpS-LBR represents a small-molecule binding domain composed of two modules, each able to bind different signal molecules. Malate and succinate were found to bind to the membrane-proximal module, whereas acetate binds to the membrane-distal module. A structural alignment of the two modules revealed that the ligand-binding sites could be superimposed and that amino acids involved in ligand recognition are conserved in both binding sites. Ligand binding to both modules was shown to trigger chemotactic responses. Further analysis showed that McpS-like receptors were found in different classes of proteobacteria, indicating that this mode of response to different carbon sources may be universally distributed. The physiological relevance of the McpS architecture may lie in its capacity to respond with high sensitivity to the preferred carbon sources malate and succinate and, at the same time, mediate lower sensitivity responses to the less preferred but very abundant carbon source acetate.
authors.affiliation: Laboratoiro de Estudios Cristalográficos, Instituto Andaluz de Ciencias de la Tierra,  Consejo Superior de Investigaciones Científicas (CSIC)-Universidad de Granada, 18100 Armilla-Granada,  Spain.
authors.forename: Estela
authors.initials: E
authors.lastname: Pineda-Molina
authors.forename: José-Antonio
authors.initials: JA
authors.lastname: Reyes-Darias
authors.forename: Jesús
authors.initials: J
authors.lastname: Lacal
authors.forename: Juan L
authors.initials: JL
authors.lastname: Ramos
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: García-Ruiz
authors.forename: Jose A
authors.initials: JA
authors.lastname: Gavira
authors.forename: Tino
authors.initials: T
authors.lastname: Krell
mesh_terms: D000085:Acetates / Q000737:chemistry / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D002633:Chemotaxis; D008024:Ligands; D008293:Malates / Q000737:chemistry / Q000378:metabolism; D017434:Protein Structure, Tertiary; D016958:Pseudomonas putida / Q000737:chemistry* / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000378:metabolism; D013386:Succinates / Q000737:chemistry / Q000378:metabolism
chemical_list: D000085:Acetates; D001426:Bacterial Proteins; D008024:Ligands; D008293:Malates; D011956:Receptors, Cell Surface; D013386:Succinates