title: [Differential expression analysis of proteins in neutrophils between clinical mastitis and healthy dairy cows].
abstract: Neutrophils provide the first line of defense against invading pathogens and have been reported to play a key role in bovine mammary immune. To examine the differential expression of proteins in neutrophils between clinical mastitis and healthy dairy cows, a 2-dimensional electrophoresis gel map with high repeatability was constructed for bovine neutrophils. From this map, seven differentially expressed proteins were identified by MALDI-TOF MS which are believed to be involved in pathways such as cell metabolism, oxidative stress, and inflammatory reaction. The differentially expressed proteins identified in this study may provide the basis for bovine mastitis resistance breeding research.
authors.affiliation: College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China. kdliu0323@126.com
authors.forename: Kai-Dong
authors.initials: KD
authors.lastname: Liu
authors.forename: Nan
authors.initials: N
authors.lastname: Liu
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: DU
authors.forename: Cai-Hong
authors.initials: CH
authors.lastname: Wei
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Guo-Bin
authors.initials: GB
authors.lastname: Lu
authors.forename: Fu-Ping
authors.initials: FP
authors.lastname: Zhao
authors.forename: Ji-Feng
authors.initials: JF
authors.lastname: Liu
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D008414:Mastitis, Bovine / Q000097:blood*; D009504:Neutrophils / Q000737:chemistry*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001798:Blood Proteins