title: Lactococcus lactis subsp. cremoris FC triggers IFN-γ production from NK and T cells via IL-12 and IL-18.
abstract: Lactic acid bacteria (LAB) benefit health as probiotics in a strain-dependent way. In this study, we investigated the immunomodulatory effects of Lactococcus lactis subsp. cremoris FC (LcFC) on dendritic cells (DCs), natural killer (NK) cells and T cells. LcFC induced the production of cytokines such as IL-10, IL-12, IL-6 and TNF-α from murine bone marrow DCs (BMDCs) via MyD88-dependent pathway. In comparison with the type strain L. lactis subsp. cremoris ATCC 19257, LcFC induced particularly high production of IL-12 while induction of IL-6 was moderate. Consequently, LcFC triggered IFN-γ production in splenic NK, CD8(+), and CD4(+) cells. Most prominent effect of LcFC on IFN-γ production was observed in NK cells, followed by CD8(+) cells, which was completely inhibited by combination of neutralizing anti-IL-12 and anti-IL-18 mAbs. Moreover, oral administration of LcFC enhanced the production of IFN-γ and IL-10 from splenocytes of treated mice. These findings suggest that this LAB strain is an efficient activator of protective cellular immunity via stimulation of myeloid cells including DCs.
authors.affiliation: Biomedial Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Akemi
authors.initials: A
authors.lastname: Kosaka
authors.forename: Huimin
authors.initials: H
authors.lastname: Yan
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ohashi
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Gotoh
authors.forename: Ami
authors.initials: A
authors.lastname: Sato
authors.forename: Hiroko
authors.initials: H
authors.lastname: Tsutsui
authors.forename: Tsuneyasu
authors.initials: T
authors.lastname: Kaisho
authors.forename: Toshiya
authors.initials: T
authors.lastname: Toda
authors.forename: Noriko M
authors.initials: NM
authors.lastname: Tsuji
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism*; D018664:Interleukin-12 / Q000235:genetics / Q000378:metabolism*; D020382:Interleukin-18 / Q000235:genetics / Q000378:metabolism*; D013294:Lactococcus lactis / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics / Q000378:metabolism; D055611:Natural Killer T-Cells / Q000378:metabolism*
chemical_list: D020382:Interleukin-18; C507780:Myd88 protein, mouse; D053594:Myeloid Differentiation Factor 88; D018664:Interleukin-12; D007371:Interferon-gamma