title: Effects of matrix metalloproteinase-1 on the myogenic differentiation of bone marrow-derived mesenchymal stem cells in vitro.
abstract: Matrix metalloproteinase-1 (MMP-1) is a member of the family of zinc-dependent endopeptidases that are capable of degrading extracellular matrix (ECM) and certain non-matrix proteins. It has been shown that MMP-1 can enhance muscle regeneration by improving the differentiation and migration of myoblasts. However, it is still not known whether MMP-1 can promote the myogenesis of bone marrow-derived mesenchymal stem cells (BMSCs). To address this question, we isolated BMSCs from C57BL/6J mice and investigated the effects of MMP-1 on their proliferation and myogenic differentiation. Our results showed that MMP-1 treatment, which had no cytotoxic effects on BMSCs, increased the mRNA and protein levels of MyoD and desmin in a dose-dependent manner, indicating that MMP-1 promoted myogenic differentiation of BMSCs in vitro. These results suggest that BMSCs may have a therapeutic potential for treating muscular disorders.
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, No. 58, Zhongshan 2nd Road, Guangzhou 510080, Guangdong Province, China.
authors.forename: Zhenyang
authors.initials: Z
authors.lastname: Zheng
authors.forename: Yan
authors.initials: Y
authors.lastname: Leng
authors.forename: Chen
authors.initials: C
authors.lastname: Zhou
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Ma
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Zhong
authors.forename: Xing-Ming
authors.initials: XM
authors.lastname: Shi
authors.forename: Weixi
authors.initials: W
authors.lastname: Zhang
mesh_terms: D050156:Adipogenesis / Q000187:drug effects; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000187:drug effects*; D002469:Cell Separation; D002478:Cells, Cultured; D003893:Desmin / Q000096:biosynthesis; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D024510:Muscle Development / Q000187:drug effects*; D017570:MyoD Protein / Q000096:biosynthesis; D010012:Osteogenesis / Q000187:drug effects
chemical_list: D003893:Desmin; D017570:MyoD Protein; D020781:Matrix Metalloproteinase 1