title: Electrocatalytic oxidation of NADH using a pencil graphite electrode modified with quercetin.
abstract: In the present study, the electrocatalytic oxidation of reduced β nicotinamide adenine dinucleotide (NADH) was investigated using a pencil graphite electrode modified with quercetin (PGE/QH(2)). The PGE/QH(2) was prepared through two steps: (i) the pre-treatment of PGE at 1.40 V vs. Ag|AgCl|KCl((sat.)) in pH 7.0 phosphate buffer containing 0.1 M KCl for 60s and (ii) adsorption of QH(2) on the PGE via immersion of PGE into a 1.0mM QH(2) solution (in ethanol) for 60s. Cyclic voltammetric studies show that the peak potential of NADH oxidation shifts from +500 mV at bare PGE to +300 mV at PGE/QH(2). The electrocatalytic currents obtained from amperometric measurements at +300 mV vs. Ag|AgCl|KCl((sat.)) and in phosphate buffer solution at pH 7.0 containing 0.1M KCl were linearly related to the concentration of NADH. Linear calibration plots are obtained in the concentration range from 0.5 μM to 100 μM. The limit of detection was found to be 0.15 μM.
authors.affiliation: Çanakkale Onsekiz Mart University, Science and Art Faculty, Department of Chemistry, TR-17100 Çanakkale, Turkey. ydilgin@yahoo.com
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Dilgin
authors.forename: Bayram
authors.initials: B
authors.lastname: Kızılkaya
authors.forename: Didem Giray
authors.initials: DG
authors.lastname: Dilgin
authors.forename: H İsmet
authors.initials: Hİ
authors.lastname: Gökçel
authors.forename: Lo
authors.initials: L
authors.lastname: Gorton
mesh_terms: D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D009243:NAD / Q000737:chemistry*; D010084:Oxidation-Reduction; D011794:Quercetin / Q000737:chemistry*
chemical_list: D009243:NAD; D006108:Graphite; D011794:Quercetin