title: Covalently linked tandem lesions in DNA.
abstract: Reactive oxygen species (ROS) generate a type of DNA damage called tandem lesions, two adjacent nucleotides both modified. A subcategory of tandem lesions consists of adjacent nucleotides linked by a covalent bond. Covalently linked tandem lesions generate highly characteristic liquid chromotography-tandem mass spectrometry (LC-MS/MS) elution profiles. We have used this property to comprehensively survey X-irradiated DNA for covalently linked tandem lesions. A total of 15 tandem lesions were detected in DNA irradiated in deoxygenated aqueous solution, five tandem lesions were detected in DNA that was irradiated in oxygenated solution.
authors.affiliation: Department of Cell Stress Biology, Roswell Park Cancer Institute, Buffalo, New York 14263, USA. harold.box@roswellpark.org
authors.forename: Helen B
authors.initials: HB
authors.lastname: Patrzyc
authors.forename: Jean B
authors.initials: JB
authors.lastname: Dawidzik
authors.forename: Edwin E
authors.initials: EE
authors.lastname: Budzinski
authors.forename: Harold G
authors.initials: HG
authors.lastname: Freund
authors.forename: John H
authors.initials: JH
authors.lastname: Wilton
authors.forename: Harold C
authors.initials: HC
authors.lastname: Box
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage; D010100:Oxygen / Q000378:metabolism; D014965:X-Rays / Q000009:adverse effects
chemical_list: D004247:DNA; D010100:Oxygen