title: Decrease in respiratory function and electron transport chain induced by airborne particulate matter (PM10) exposure in lung mitochondria.
abstract: Particulate matter, with a mean aerodynamic diameter of ≤10 µm (PM10), exposure is considered as a risk factor for cardiovascular and respiratory diseases. The mechanism of cell damage induced by PM10 exposure is related to mitochondrial alterations. The aim of this work was to investigate the detailed alterations induced by PM10 on mitochondrial function. Since lung tissue is one of the most important targets of PM10 inhalation, isolated mitochondria from lung rat tissue were exposed to PM10 and structural alterations were analyzed by transmission electron microscopy. Mitochondrial function was evaluated by respiratory control index (RCI), membrane potential, adenosine triphosphate (ATP) synthesis, and activity of respiratory chain. Results showed that exposure to PM10 in isolated mitochondria from lung tissue caused enlarged intermembrane spaces and shape alterations, disruption of cristae, and the decrease in dense granules. Oxygraphic traces showed a concentration-dependent decrease in oxygen consumption and RCI. In addition, mitochondrial membrane potential, ATP synthesis, and activity of complexes II and IV showed an increase and decrease, respectively, after PM10 exposure. PM10 exposure induced disruption in structure and function in isolated mitochondria from lung rat tissue.
authors.affiliation: Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Estado de México, México.
authors.forename: Norma Laura
authors.initials: NL
authors.lastname: Delgado-Buenrostro
authors.forename: Verónica
authors.initials: V
authors.lastname: Freyre-Fonseca
authors.forename: Claudia M García
authors.initials: CM
authors.lastname: Cuéllar
authors.forename: Yesennia
authors.initials: Y
authors.lastname: Sánchez-Pérez
authors.forename: Emma Berta
authors.initials: EB
authors.lastname: Gutierrez-Cirlos
authors.forename: Tecilli
authors.initials: T
authors.lastname: Cabellos-Avelar
authors.forename: Marisol
authors.initials: M
authors.lastname: Orozco-Ibarra
authors.forename: José
authors.initials: J
authors.lastname: Pedraza-Chaverri
authors.forename: Yolanda I
authors.initials: YI
authors.lastname: Chirino
mesh_terms: D000393:Air Pollutants / Q000633:toxicity; D000704:Analysis of Variance; D000818:Animals; D004579:Electron Transport / Q000187:drug effects*; D003576:Electron Transport Complex IV / Q000378:metabolism; D019570:Inhalation Exposure / Q000032:analysis*; D008168:Lung / Q000166:cytology / Q000187:drug effects*; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000187:drug effects* / Q000473:pathology; D010101:Oxygen Consumption / Q000187:drug effects; D052638:Particulate Matter / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013385:Succinate Dehydrogenase / Q000378:metabolism
chemical_list: D000393:Air Pollutants; D052638:Particulate Matter; D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV
keywords: PM10; mitochondrial function; respiratory chain.