title: Fluorescent primer-based in vitro transcription system of viral RNA-dependent RNA polymerases.
abstract: Viral infection is a leading cause of disease and death. Although vaccines are the most effective method of controlling viral infections, antiviral drugs are also important. Here, we established an in vitro transcription system by using fluorescein isothiocyanate-conjugated primers for RNA polymerases of viruses that are important disease-causing human pathogens (influenza, hepatitis C, Japanese encephalitis viruses, and enterovirus 71). This technology will allow us to analyze RNA polymerase activity without using radioisotopes.
authors.affiliation: Unit of Viral Genome Regulation, Key Laboratory of Molecular Virology and Immunology, Institute Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200025, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Leiyun
authors.initials: L
authors.lastname: Weng
authors.forename: Hongbing
authors.initials: H
authors.lastname: Jiang
authors.forename: Shijian
authors.initials: S
authors.lastname: Zhang
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Toyoda
mesh_terms: D002474:Cell-Free System; D017931:DNA Primers / Q000737:chemistry*; D005453:Fluorescence; D006801:Humans; D012328:RNA Viruses / Q000201:enzymology*; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000737:chemistry*
chemical_list: D017931:DNA Primers; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase