title: Molecular cloning and expression of 17β-hydroxysteroid dehydrogenase type 2 gene in Hu sheep.
abstract: 17β-Hydroxysteroid dehydrogenase type 2 (17β-HSD2) catalyzes the NADP+-dependent oxidation of the most potent estrogen 17β-estradiol into the weak estrogen estrone, and the conversion of testosterone to androstenedione. It has been reported that 17β-HSD2 was expressed in many tissues in human, rats, however, the full-length sequence of 17β-HSD2 gene and its expression in ewe were still unknown. In this study, we cloned the full-length cDNA sequence and investigated mRNA differential expression in 28 tissues of 12 adult Hu-Sheep which were fed with high- and low- dietary intake. The 1,317 bp full-length cDNA sequence was first cloned. The coding region was 1,167 bp in length, and the monomer was estimated to contain 389 amino acid residues. It shares high AA sequence identity with that of bos Taurus (96.13 %), sus scrofa (77.06 %), canis lupus familiaris (70.44 %), Callithrix jacchus (65.72 %), Nomascus leucogenys (65.46 %), pan troglodytes (65.21 %), human (64.69 %), mus musculus (58.35 %), and a comparatively lower identity to danio rerio (37.85 %). 17β-HSD2 gene was high expressed in gastrointestinal (GI) tract, liver, but weakly expressed in other tissues. No detected expression was examined in lung. 17β-HSD2 gene expression was significantly difference in rumen, omasum, duodenum, cecum, hypophysis after high- and low- dietary intake. Results from the present study suggested that 17β-HSD2 plays a crucial role in almost all tissues protecting against excessive levels of active steroid hormone, and GI tract maybe an important steroid hormone metabolizing organ in Hu-Sheep. This present study is the first to provide the primary foundation for further insight into this ovine gene.
authors.affiliation: Institute of Sheep & Goat Science, and Center of Animal Embryo Engineering & Technology, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People's Republic of China. wangchanglong921@163.com
authors.forename: Chang Long
authors.initials: CL
authors.lastname: Wang
authors.forename: Shi Jia
authors.initials: SJ
authors.lastname: Ying
authors.forename: Zi Yu
authors.initials: ZY
authors.lastname: Wang
authors.forename: Hui Jun
authors.initials: HJ
authors.lastname: Xing
authors.forename: Li Zhong
authors.initials: LZ
authors.lastname: Wang
authors.forename: Dong Yang
authors.initials: DY
authors.lastname: He
authors.forename: Shen Hua
authors.initials: SH
authors.lastname: Xiao
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002432:Cecum / Q000201:enzymology; D003001:Cloning, Molecular; D004386:Duodenum / Q000201:enzymology; D002149:Energy Intake; D005260:Female; D015870:Gene Expression; D008969:Molecular Sequence Data; D009851:Omasum / Q000201:enzymology; D009928:Organ Specificity; D010802:Phylogeny; D010902:Pituitary Gland / Q000201:enzymology; D012417:Rumen / Q000201:enzymology; D017422:Sequence Analysis, DNA; D012756:Sheep / Q000235:genetics* / Q000378:metabolism
chemical_list: D015067:17-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase