title: Simultaneous determination of 13 components in oxidative hair dyes by high-performance chromatography using an ion-pair reagent.
abstract: A reliable high-performance liquid chromatography (HPLC) method was developed for the simultaneous determination of 13 dye intermediates, including benzenediamines, aminophenols, benzenediols, naphthalenediol, and diaminopyridine, in oxidative hair dyes. Samples were extracted with 50% ethanol by adding sodium dithionite to prevent oxidation. The influences of buffer type, buffer pH, ion-pair reagent, and elution gradient were studied. A C18 column with aqueous compatibility and acetonitrile-citric acid mobile phase system (pH 2.6) with sodium 1-octanesulfonate as ion-pair reagent were selected for the separation of target compounds. Detection was performed by a diode array detector, (DAD) and two different wavelengths (280 and 331 nm) were used for quantification. Results showed that 13 dye intermediates got good separation within 25 min. The detection limits of these compounds were in the range of 0.2-2 mg/l. The calibration curves were linear within 2-500 mg/l with 0.999 as a typical correlation coefficient. The recoveries of target compounds in hair dyes ranged from 81.7% to 102.0% with four addition levels. The method described was validated by five different laboratories and successfully applied to the analysis of commercial oxidative hair dyes.
authors.affiliation: Technology Center of Xiamen Entry-Exit Inspection and Quarantine Bureau, Xiamen, People's Republic of China. laiy@xmciq.gov.cn
authors.forename: Ying
authors.initials: Y
authors.lastname: Lai
authors.forename: Honghui
authors.initials: H
authors.lastname: Wang
authors.forename: Qingmu
authors.initials: Q
authors.lastname: Dong
authors.forename: Hexiu
authors.initials: H
authors.lastname: Chen
authors.forename: Rui
authors.initials: R
authors.lastname: Lin
authors.forename: Yanping
authors.initials: Y
authors.lastname: Cai
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006202:Hair Dyes / Q000737:chemistry*; D016877:Oxidants / Q000737:chemistry*; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D006202:Hair Dyes; D016877:Oxidants