title: Eleven microsatellite markers for the mountain clover Trifolium montanum (Fabaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed in Trifolium montanum to investigate pollen dispersal patterns at landscape scale with a pollen-pool analysis (indirect parentage analysis) as well as in an experimental set-up with a direct paternity analysis.
  
  
  RESULTS
  Screening 46 microsatellites developed for T. repens yielded four markers usable in T. montanum. Seven additional ones have been developed specifically for the target species using a 454-sequencing approach. All markers were polymorphic, with an allele number ranging from two to 45 based upon 254 individuals sampled from four populations, and an exclusion probability of 0.999.
  
  
  CONCLUSIONS
  These markers proved a useful and reliable molecular tool for use in population genetics and parentage studies of this common grassland herb.
authors.affiliation: Institute of Terrestrial Ecosystems (ITES)-Ecosystem Management, Building CHN Floor G, ETH Zürich, Universitaetstrasse 16, 8092 Zürich, Switzerland. philippe.matter@env.ethz.ch
authors.forename: Philippe
authors.initials: P
authors.lastname: Matter
authors.forename: Kirsti
authors.initials: K
authors.lastname: Määttänen
authors.forename: Chris J
authors.initials: CJ
authors.lastname: Kettle
authors.forename: Jaboury
authors.initials: J
authors.lastname: Ghazoul
authors.forename: Andrea R
authors.initials: AR
authors.lastname: Pluess
mesh_terms: D000531:Altitude; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D015698:Genomic Library; D005838:Genotype; D005843:Geography; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013557:Switzerland; D029921:Trifolium / Q000145:classification / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018744:DNA, Plant