title: Tissue microarray-based evaluation of Chromatin Assembly Factor-1 (CAF-1)/p60 as tumour prognostic marker.
abstract: In this study we aimed to confirm the emerging role of Chromatin Assembly Factor 1 (CAF-1 p60) as a new proliferation and prognostic marker for cancer and to test the usefulness of the tissue microarray technique (TMA) for CAF-1 p60 rapid screening in several human malignancies. CAF-1 is a histone chaperone, regulating chromatin dynamics during DNA replication and repair in eukaryotics. TMA is a powerful high-throughput methodology in the study of cancer, allowing simultaneous assessment of different biomarkers within large numbers of tissue specimens. We generated TMA taking 3 mm diameter-core biopsies from oral squamous cell carcinoma, prostate cancer, salivary gland tumours and skin melanoma specimens, which had been previously tested for CAF-1 p60 on routine tissue sections. We also analysed, for the first time, 30 larynx and 30 skin squamous cell carcinomas. CAF-1 p60 resulted over-expressed in both the tissue sections and the TMA specimens, with the highest levels of expression in tumours which were more aggressive and metastasizing. Notably, a high degree of agreement was found between the CAF-1 p60 assessment on TMAs and on routine tissue sections. Our findings confirm the prognostic role of CAF-1 p60 and indicate TMA as a really advantageous method for CAF-1 p60 immunohistochemical screening, allowing savings on both tissue quantity and operator-time.
authors.affiliation: Department of Biomorphological and Functional Sciences, Pathology Section, University of Naples "Federico II", Naples 80138, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Mascolo
authors.affiliation: Department of Biomorphological and Functional Sciences, Pathology Section, University of Naples "Federico II", Naples 80138, Italy.
authors.forename: Gennaro
authors.identifier: 0000-0002-9354-4087
authors.initials: G
authors.lastname: Ilardi
authors.affiliation: Department of Biomorphological and Functional Sciences, Pathology Section, University of Naples "Federico II", Naples 80138, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Merolla
authors.affiliation: Department of Biomorphological and Functional Sciences, Pathology Section, University of Naples "Federico II", Naples 80138, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Russo
authors.affiliation: Department of Biomorphological and Functional Sciences, Pathology Section, University of Naples "Federico II", Naples 80138, Italy.
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Vecchione
authors.affiliation: Department of Biomorphological and Functional Sciences, Pathology Section, University of Naples "Federico II", Naples 80138, Italy.
authors.forename: Gaetano
authors.initials: G
authors.lastname: De Rosa
authors.affiliation: Department of Biomorphological and Functional Sciences, Pathology Section, University of Naples "Federico II", Naples 80138, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Staibano
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics / Q000401:mortality; D056504:Chromatin Assembly Factor-1 / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000401:mortality; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness / Q000235:genetics; D009369:Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000401:mortality; D011379:Prognosis; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000401:mortality; D012468:Salivary Gland Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000401:mortality; D012878:Skin Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000401:mortality; D046888:Tissue Array Analysis / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D056504:Chromatin Assembly Factor-1
keywords: Chromatin Assembly Factor-1; cancer screening; immunohistochemistry; tissue microarray