title: Proliferation and migration of tumor cells in tapered channels.
abstract: Tumor cells depict two deviant tendencies; over-proliferation and vigorous migration. A tapered channel device is designed and fabricated for in vitro studies. We report inhibited proliferation and migration of human glioblastoma (hGBM) cells when exposed to an aptamer that is known to bind epidermal growth factor receptors (EGFR). The device is integrated with controlled ambient and microscope for providing real-time and quantitative characterization of the tumor cell behavior. The results show that hGBM cells loose proliferation and motility when exposed to the anti-EGFR aptamer. The aptamer directly inhibits and blocks EGF-induced EGFR phosphorylation. This also reduces the ability of cells to remodel their internal structure for invasion through narrow constrictions. This provides a framework for possible studies on efficacy of other inhibiting molecules.
authors.affiliation: Nano-Bio Lab, University of Texas at Arlington, 500 S. Cooper St, M.S. 19072, Room #217, Arlington, TX, 76019, USA.|Department of Bioengineering, University of Texas at Arlington, Arlington, TX, 76019, USA.|Nanotechnology Research and Education Center, University of Texas at Arlington, Arlington, TX, 76019, USA.|Mawson Institute, University of South Australia, Mawson Lakes, Adelaide, SA, 5095, Australia.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wan
authors.affiliation: Department of Bioengineering, University of Texas at Arlington, Arlington, TX, 76019, USA.
authors.forename: Deepika
authors.initials: D
authors.lastname: Tamuly
authors.affiliation: Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, TX, USA.
authors.forename: Peter B
authors.initials: PB
authors.lastname: Allen
authors.affiliation: Department of Bioengineering, University of Texas at Arlington, Arlington, TX, 76019, USA.|Nanotechnology Research and Education Center, University of Texas at Arlington, Arlington, TX, 76019, USA.
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Kim
authors.affiliation: Annette G. Strauss Center for Neuro-Oncology, University of Texas Southwestern Medical Center, Dallas, TX, USA.|Department of Neurology, University of Texas Southwestern Medical Center, Dallas, TX, USA.|Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Bachoo
authors.affiliation: Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, TX, USA.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Ellington
authors.affiliation: Nano-Bio Lab, University of Texas at Arlington, 500 S. Cooper St, M.S. 19072, Room #217, Arlington, TX, 76019, USA. smiqbal@uta.edu.|Department of Bioengineering, University of Texas at Arlington, Arlington, TX, 76019, USA. smiqbal@uta.edu.|Nanotechnology Research and Education Center, University of Texas at Arlington, Arlington, TX, 76019, USA. smiqbal@uta.edu.|Department of Electrical Engineering, University of Texas at Arlington, Arlington, TX, 76019, USA. smiqbal@uta.edu.|Joint Graduate Committee of Bioengineering Program, University of Texas at Arlington and University of Texas Southwestern Medical Center at Dallas, University of Texas at Arlington, Arlington, TX, 76019, USA. smiqbal@uta.edu.
authors.forename: Samir M
authors.initials: SM
authors.lastname: Iqbal
mesh_terms: D052157:Aptamers, Nucleotide / Q000378:metabolism; D032701:Biomimetics / Q000379:methods*; D002455:Cell Division; D002465:Cell Movement; D049109:Cell Proliferation; D066246:ErbB Receptors / Q000378:metabolism; D005909:Glioblastoma / Q000473:pathology*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D010766:Phosphorylation; D015398:Signal Transduction
chemical_list: D052157:Aptamers, Nucleotide; C512478:EGFR protein, human; D066246:ErbB Receptors