title: Leptin modulates lymphocytes' adherence to hepatic stellate cells is associated with oxidative status alterations.
abstract: We investigated leptin effects on lymphocyte interactions with hepatic-stellate-cells (HSCs). Leptin showed pro-fibrotic effects on HSCs with oxidative status imbalance. In co-cultures, leptin activates HSCs and consequently adhered HCV-lymphocytes more than healthy ones. Leptin also increased healthy and HCV lymphocyte proliferations; increased their reactive-oxygen-species; decreased antioxidants (reduced-glutathione) levels while inhibited apoptosis only of HCV-lymphocytes. The leptin-treated HCV-lymphocytes activated HSCs, increase interleukin-4 while decreased their apoptosis. Leptin-receptor-deficient (db-db)-HSCs did not adhere lymphocytes. db/db-lymphocytes however showed fewer adherences to HSCs when compared to WT-counterparts. This study presents immune and oxidative modulatory effects of leptin on lymphocytes and their consequent interaction with HSCs.
authors.affiliation: Liver Unit, Hadassah Medical Center, Jerusalem, Israel.
authors.forename: Lina
authors.initials: L
authors.lastname: Abu-Tair
authors.forename: Sarit
authors.initials: S
authors.lastname: Doron
authors.forename: Mahmud
authors.initials: M
authors.lastname: Mahamid
authors.forename: Johnny
authors.initials: J
authors.lastname: Amer
authors.forename: Rifaat
authors.initials: R
authors.lastname: Safadi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects*; D049109:Cell Proliferation; D019069:Cell Respiration / Q000187:drug effects*; D002478:Cells, Cultured; D018920:Coculture Techniques; D055166:Hepatic Stellate Cells / Q000276:immunology* / Q000502:physiology*; D006801:Humans; D020738:Leptin / Q000378:metabolism*; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D020738:Leptin; D017382:Reactive Oxygen Species
keywords: Adherence; CCl(4); GSH; HCV; HSCs; Hepatic fibrosis; Leptin receptor; Lymphocytes; Oxidative stress; PBL; ROS; carbon-tetrachloride; hepatic-stellate-cells; hepatitis-C-virus; peripheral-blood-lymphocytes; reactive oxygen species; reduced glutathione