title: Sgk1 enhances RANBP1 transcript levels and decreases taxol sensitivity in RKO colon carcinoma cells.
abstract: The serum- and glucocorticoid-regulated kinase (Sgk1) is essential for hormonal regulation of epithelial sodium channel-mediated sodium transport and is involved in the transduction of growth factor-dependent cell survival and proliferation signals. Growing evidence now points to Sgk1 as a key element in the development and/or progression of human cancer. To gain insight into the mechanisms through which Sgk1 regulates cell proliferation, we adopted a proteomic approach to identify up- or downregulated proteins after Sgk1-specific RNA silencing. Among several proteins, the abundance of which was found to be up- or downregulated upon Sgk1 silencing, we focused our attention of RAN-binding protein 1 (RANBP1), a major effector of the GTPase RAN. We report that Sgk1-dependent regulation of RANBP1 has functional consequences on both mitotic microtubule activity and taxol sensitivity of cancer cells.
authors.affiliation: Department of Human Health, University Magna Graecia at Catanzaro, Campus S Venuta, Località Germaneto Viale Europa, Catanzaro, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Amato
authors.forename: D
authors.initials: D
authors.lastname: Scumaci
authors.forename: L
authors.initials: L
authors.lastname: D'Antona
authors.forename: R
authors.initials: R
authors.lastname: Iuliano
authors.forename: M
authors.initials: M
authors.lastname: Menniti
authors.forename: M
authors.initials: M
authors.lastname: Di Sanzo
authors.forename: M C
authors.initials: MC
authors.lastname: Faniello
authors.forename: E
authors.initials: E
authors.lastname: Colao
authors.forename: P
authors.initials: P
authors.lastname: Malatesta
authors.forename: A
authors.initials: A
authors.lastname: Zingone
authors.forename: V
authors.initials: V
authors.lastname: Agosti
authors.forename: F S
authors.initials: FS
authors.lastname: Costanzo
authors.forename: A M
authors.initials: AM
authors.lastname: Mileo
authors.forename: M G
authors.initials: MG
authors.lastname: Paggi
authors.forename: F
authors.initials: F
authors.lastname: Lang
authors.forename: G
authors.initials: G
authors.lastname: Cuda
authors.forename: P
authors.initials: P
authors.lastname: Lavia
authors.forename: N
authors.initials: N
authors.lastname: Perrotti
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism*; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000235:genetics* / Q000378:metabolism*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017874:Immediate-Early Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics*; D017239:Paclitaxel / Q000494:pharmacology; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D040901:Proteomics; D034622:RNA Interference; D016329:Sp1 Transcription Factor / Q000378:metabolism; D014158:Transcription, Genetic; D000099184:Serum-Glucocorticoid Regulated Kinases
chemical_list: D017874:Immediate-Early Proteins; D009687:Nuclear Proteins; D017239:Paclitaxel; D017346:Protein Serine-Threonine Kinases; D016329:Sp1 Transcription Factor; D000099184:Serum-Glucocorticoid Regulated Kinases; C091775:ran-binding protein 1