title: Clinical significance of mycobacterial genotyping in Mycobacterium avium lung disease in Korea.
abstract: METHODS
  A recent study in Japan found that mycobacterial genotyping was associated with disease progression and susceptibility to certain drugs in Mycobacterium avium lung disease. However, it is not known whether this association is true in other populations.
  
  
  OBJECTIVE
  To investigate the association between mycobacterial genotype, clinical characteristics and the progression of M. avium lung disease in Korean patients.
  
  
  METHODS
  A total of 102 M. avium clinical isolates were genotyped using M. avium tandem repeats-variable number of  tandem repeats (MATR-VNTR).
  
  
  RESULTS
  MATR-VNTR typing demonstrated a high discriminatory power and genetic diversity for molecular epidemiological studies of M. avium. In the phylogenetic tree, the M. avium clinical isolates were divided into three major clusters: A, B and  C. Cluster A was observed most frequently (64/102, 63%), whereas cluster C was found in a minor proportion of the isolates (8/102, 8%). However, there was no association between the clinical characteristics, disease progression and drug susceptibility and the phylogenetic tree based on VNTR  genotyping.
  
  
  CONCLUSIONS
  MATR-VNTR genotyping may be useful for epidemiological studies of M. avium lung disease; however, no association was found between the specific VNTR genotypes of M. avium and the clinical characteristics of Korean patients.
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: S-Y
authors.initials: SY
authors.lastname: Kim
authors.forename: S-T
authors.initials: ST
authors.lastname: Lee
authors.forename: B-H
authors.initials: BH
authors.lastname: Jeong
authors.forename: K
authors.initials: K
authors.lastname: Jeon
authors.forename: J-W
authors.initials: JW
authors.lastname: Kim
authors.forename: S J
authors.initials: SJ
authors.lastname: Shin
authors.forename: W-J
authors.initials: WJ
authors.lastname: Koh
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D005838:Genotype; D006801:Humans; D015994:Incidence; D008171:Lung Diseases / Q000453:epidemiology / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D018598:Minisatellite Repeats; D009162:Mycobacterium avium / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D056910:Republic of Korea / Q000453:epidemiology
chemical_list: D004269:DNA, Bacterial