title: Influence of blood specimen collection method on various preanalytical sample quality indicators.
abstract: Preanalytical errors contribute to a large proportion of total laboratory errors. In order to achieve continuous laboratory improvement, it is important to focus on all phases of patient specimen testing i.e. preanalytical, analytical and post-analytical. With large variations in the way venous blood specimens are collected using diverse devices in the country, the effect of such practices on specimen quality is not known. The purpose of this study was to monitor fourteen specimen preanalytical quality indicators in order to compare the usage of evacuated blood collection devices with needle and syringe open collection using either disposable tubes or re-washed glass vials. The study involved 26638 patient specimens assessed over a period of 6 months. The results demonstrated that evacuated closed blood collection resulted in an approximate 100-fold reduction in the incidence of hemolysis in samples. Similarly, there was a 200-fold reduction in incidence of insufficient specimen quantity while using evacuated collection system. It was also found that incidence of specimen contamination, improper volume of sample collected, and specimen spillage was also lower when the evacuated collection system was used. Further, it was also observed that the facility with a laboratory information system demonstrated much lower specimen identification and related errors. The observed results clearly demonstrate that the usage of the evacuated blood collection system resulted in improvement of preanalytical specimen quality as compared to needle and syringe usage.
authors.affiliation: Department of Lab. Medicine, Jt. Dir-Research, P.D. Hinduja National Hospital & Medical Research Center, Mahim, Mumbai, 400016 India.
authors.forename: Tester F
authors.initials: TF
authors.lastname: Ashavaid
authors.forename: Sucheta P
authors.initials: SP
authors.lastname: Dandekar
authors.forename: Bhamini
authors.initials: B
authors.lastname: Keny
authors.forename: Vishaal R
authors.initials: VR
authors.lastname: Bhambhwani
keywords: Hemolysis; Needle and Syringe; Open collection; Preanalytical; Total quality; Vacutainer