title: Expression of hsp70, hsp100 and ubiquitin in Aloe barbadensis Miller under direct heat stress and under temperature acclimation conditions.
abstract: KEY MESSAGE                 :                                     The study determined the tolerance of Aloe vera to high temperature, focusing on the expression of                    hsp70                   ,                   hsp100                    and                   ubiquitin                    genes. These were highly expressed in plants acclimated at 35 °C prior to a heat shock of 45 °C.                                                                                     Aloe barbadensis Miller (Aloe vera), a CAM plant, was introduced into Chile in the semiarid IV and III Regions, which has summer diurnal temperature fluctuations of 25 to 40 °C and annual precipitation of 40 mm (dry years) to 170 mm (rainy years). The aim of this study was to investigate how Aloe vera responds to water and heat stress, focusing on the expression of heat shock genes (hsp70, hsp100) and ubiquitin, which not studied before in Aloe vera. The LT(50) of Aloe vera was determined as 53.2 °C. To study gene expression by semi-quantitative RT-PCR, primers were designed against conserved regions of these genes. Sequencing the cDNA fragments for hsp70 and ubiquitin showed a high identity, over 95 %, with the genes from cereals. The protein sequence of hsp70 deduced from the sequence of the cDNA encloses partial domains for binding ATP and the substrate. The protein sequence of ubiquitin deduced from the cDNA encloses a domain for interaction with the enzymes E2, UCH and CUE. The expression increased with temperature and water deficit. Hsp70 expression at 40-45 °C increased 50 % over the controls, while the expression increased by 150 % over the controls under a water deficit of 50 % FC. The expression of all three genes was also studied under 2 h of acclimation at 35 or 40 °C prior to a heat shock at 45 °C. Under these conditions, the plants showed greater expression of all genes than when they were subjected to direct heat stress.
authors.affiliation: Laboratorio de Biología Molecular Vegetal Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile.
authors.forename: Claudia
authors.initials: C
authors.lastname: Huerta
authors.forename: Matías
authors.initials: M
authors.lastname: Freire
authors.forename: Liliana
authors.initials: L
authors.lastname: Cardemil
mesh_terms: D000064:Acclimatization; D000504:Aloe / Q000235:genetics* / Q000502:physiology; D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D003681:Dehydration; D018506:Gene Expression Regulation, Plant; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D018869:Heat-Shock Response; D006358:Hot Temperature; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D017422:Sequence Analysis, DNA; D025801:Ubiquitin / Q000235:genetics* / Q000378:metabolism
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D025801:Ubiquitin