title: Staining and embedding the whole mouse brain for electron microscopy.
abstract: The development of methods for imaging large contiguous volumes with the electron microscope could allow the complete mapping of a whole mouse brain at the single-axon level. We developed a method based on prolonged immersion that enables staining and embedding of the entire mouse brain with uniform myelin staining and a moderate preservation of the tissue's ultrastructure. We tested the ability to follow myelinated axons using serial block-face electron microscopy.
authors.affiliation: Max-Planck Institute for Medical Research, Heidelberg, Germany. Shawn.Mikula@mpimf-heidelberg.mpg.de
authors.forename: Shawn
authors.initials: S
authors.lastname: Mikula
authors.forename: Jonas
authors.initials: J
authors.lastname: Binding
authors.forename: Winfried
authors.initials: W
authors.lastname: Denk
mesh_terms: D000818:Animals; D001369:Axons / Q000648:ultrastructure; D001921:Brain / Q000648:ultrastructure*; D008297:Male; D051379:Mice; D008854:Microscopy, Electron / Q000379:methods*; D009413:Nerve Fibers, Myelinated / Q000648:ultrastructure; D009993:Osmium Tetroxide; D013194:Staining and Labeling / Q000379:methods*; D016610:Tissue Embedding / Q000379:methods*
chemical_list: D009993:Osmium Tetroxide