title: Photoresponsive capture and release of lectins in multilamellar complexes.
abstract: The development of triggered release systems for delivery of peptides and proteins is critical to the success of biological drug therapies. In this paper we describe a dynamic supramolecular system able to capture and release proteins in response to light. The ternary system self-assembles in a dilute aqueous solution of three components: vesicles of amphiphilic cyclodextrin host, noncovalent cross-linkers with an azobenzene and a carbohydrate moiety, and lectins. The cross-linkers form inclusion complexes with the host vesicles, provided the azobenzene is in the trans state. The formation of a ternary complex with lectins requires a high density of cross-linkers on the surface of vesicles. The key innovation in this system is a photoinduced switch from a multivalent, high-affinity state that captures protein to a monovalent, low-affinity state that releases protein. By using isothermal titration calorimetry, dynamic light scattering, UV/vis spectroscopy, and cryogenic transmission electron microscopy, we demonstrate that photoinduced capture and release of lectins in dense multilamellar complexes is highly efficient, highly selective, and fully reversible.
authors.affiliation: Organic Chemistry Institute and Graduate School of Chemistry, Westfälische Wilhelms-Universität Münster, Corrensstrasse 40, 48149 Münster, Germany.
authors.forename: Avik
authors.initials: A
authors.lastname: Samanta
authors.forename: Marc C A
authors.initials: MC
authors.lastname: Stuart
authors.forename: Bart Jan
authors.initials: BJ
authors.lastname: Ravoo
mesh_terms: D001391:Azo Compounds / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D003505:Cyclodextrins / Q000737:chemistry; D016503:Drug Delivery Systems; D037102:Lectins / Q000737:chemistry*; D008027:Light; D008051:Lipid Bilayers / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D013816:Thermodynamics
chemical_list: D001391:Azo Compounds; D003432:Cross-Linking Reagents; D003505:Cyclodextrins; D037102:Lectins; D008051:Lipid Bilayers; C009850:azobenzene