title: Dual-signal amplification strategy for ultrasensitive photoelectrochemical immunosensing of α-fetoprotein.
abstract: An ultrasensitive photoelectrochemical immunoassay of cancer biomarker α-fetoprotein (AFP) is proposed that uses titanium dioxide (TiO(2)) coupled with AFP-CdTe-GOx bioconjugate, which featured AFP antigen and glucose oxidase (GOx) labels linked to CdTe quantum dots (QDs) for signal amplification. The synthesized CdTe QDs yielded a homogeneous and narrow size distribution, which allowed the binding of AFP and GOx on CdTe QDs. Greatly enhanced sensitivity for AFP came from a dual signal amplification strategy. First, an effective matching of energy levels between the conduction bands of CdTe QDs and TiO(2) allowed for fast electron injection from excited CdTe QDs to TiO(2) upon irradiation, which reduced the recombination process of electron-hole pairs and prompted photoelectrochemical performance. Second, GOx enzyme could catalyze glucose to produce H(2)O(2), which acted as a sacrificial electron donor to scavenge the photogenerated holes in the valence band of CdTe QDs, further causing an enhanced photocurrent. Thus, on the basis of the dual signal amplification strategy, the competitive immunosensor based on the specific binding of anti-AFP antibodies to AFP and AFP-CdTe-GOx bioconjugates was achieved. This proposed biosensor for AFP possessed largely increased linear detection range from 0.5 pg/mL to 10 μg/mL with a detection limit of 0.13 pg/mL. The proposed amplification strategy shows high sensitivity, stability, and reproducibility and can become a promising platform for other protein detection.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, People's Republic of China.
authors.forename: Yong-Jie
authors.initials: YJ
authors.lastname: Li
authors.forename: Meng-Jie
authors.initials: MJ
authors.lastname: Ma
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhu
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D004566:Electrodes; D005949:Glucose Oxidase / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007118:Immunoassay; D057230:Limit of Detection; D008113:Liver Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D055668:Photochemical Processes; D045663:Quantum Dots; D014025:Titanium / Q000737:chemistry / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D014408:Biomarkers, Tumor; D000509:alpha-Fetoproteins; C009495:titanium dioxide; D006861:Hydrogen Peroxide; D014025:Titanium; D005949:Glucose Oxidase