title: Artificial microRNAs for specific gene silencing in rice.
abstract: Artificial microRNAs (amiRNAs) have been shown to facilitate efficient gene silencing with high specificity to the intended target gene(s). For the plant breeder, gene silencing by artificial miRNAs will certainly accelerate gene discovery, because it allows targeting of all genes in a mapping interval, independent of the genetic background. In addition, beneficial knockout phenotypes can easily be transferred between varieties and across incompatibility barriers. This chapter describes the generation and application of amiRNAs as a gene silencing tool in rice.
authors.affiliation: Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tübingen, Germany. norman@warthmann.com
authors.forename: Norman
authors.initials: N
authors.lastname: Warthmann
authors.forename: Stephan
authors.initials: S
authors.lastname: Ossowski
authors.forename: Rebecca
authors.initials: R
authors.lastname: Schwab
authors.forename: Detlef
authors.initials: D
authors.lastname: Weigel
mesh_terms: D003001:Cloning, Molecular / Q000379:methods; D019295:Computational Biology / Q000379:methods; D020868:Gene Silencing; D035683:MicroRNAs / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified; D012984:Software; D014170:Transformation, Genetic
chemical_list: D035683:MicroRNAs