title: [Effect of calcitonin gene-related peptide on the maturation of oocyte in vitro].
abstract: OBJECTIVE
  To explore the effect of calcitonin gene-related peptide (CGRP) on murine oocyte maturation.
  
  
  METHODS
  After injection of pregnant mare serum gonadotropin (PMSG, 10 U, i.p.) for 48 h, 6-week old female Kunming mice were killed, and the cumulus oocyte complexes (COCs) were collected from ovaries and inoculated in the culture plate by 30-40/hole. The COCs were treated with 4 concentrations of CGRP (0, 10(-10), 10(-9), and 10(-8) mol/L), and the germinal vesicle breakdown (GVBD) and polar body I (PBI) were examined. Human granulosa cells were also cultured with CGRP (0, 10(-10), 10(-9), 10(-8) mol/L) and levels of intracellular cyclic adenosine monophosphate (cAMP) were measured.
  
  
  RESULTS
  Exogenous CGRP caused a decrease in GVBD and PBI in COCs, and an increase in cAMP levels in human granulosa cells in a concentration-dependent manner.
  
  
  CONCLUSIONS
  CGRP can inhibit the oocyte maturation, which may be related to the increased content of cAMP in granulosa cells.
authors.affiliation: Institute of Reproduction and Stem Cell Engineering, Central South University, Changsha, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.forename: Hongqing
authors.initials: H
authors.lastname: Liao
authors.forename: Shuoping
authors.initials: S
authors.lastname: Zhang
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Xu
authors.forename: Fei
authors.initials: F
authors.lastname: Gong
authors.forename: Guangxiu
authors.initials: G
authors.lastname: Lu
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D006107:Granulosa Cells / Q000166:cytology; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects
chemical_list: D000242:Cyclic AMP; D015740:Calcitonin Gene-Related Peptide