title: One-year monitoring of aflatoxins and ochratoxin A in tiger-nuts and their beverages.
abstract: A sensitive and selective liquid chromatography-triple quadrupole-tandem mass spectrometry (LC-ESI-MS-MS) method was developed for the routine analysis of aflatoxins (AFB(1), AFB(2), AFG(1) and AFG(2)) and ochratoxin A (OTA) in tiger nuts and tiger-nut beverage (horchata). A matrix solid phase dispersion was adapted to eliminate lipidic interferences. The solid support was C(18), while the elution solvent was acetonitrile. Mean recoveries obtained at two fortification levels were 72-83% and 71-81% for horchata and tiger nut respectively with relative standard deviations (RSDs) <13% and 15% respectively. The LC-MS-MS method allowed quantification and identification at low levels in two matrices. The method was applied for the routine analysis of tiger-nuts and horchata samples collected from different supermarkets of Valencia (Spain) during one year (March 2009-March 2010). A total of 238 samples were analysed and 32 samples were found positives for OTA, AFB(1), AFB(2) and AFG(2).
authors.affiliation: Departament de Medicina Preventiva, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Rubert
authors.forename: N
authors.initials: N
authors.lastname: Sebastià
authors.forename: J M
authors.initials: JM
authors.lastname: Soriano
authors.forename: C
authors.initials: C
authors.lastname: Soler
authors.forename: J
authors.initials: J
authors.lastname: Mañes