title: Different gene transfer methods at the very early, early, late and whole embryonic stages in chicken.
abstract: New technologies in gene transfer combined with experimental embryology make the chicken embryo an excellent model system for gene function studies. The techniques of in ovo electroporation, in vitro culture for ex ovo electroporation and retrovirus-mediated gene transfer have already been fully developed in chicken. Yet to our knowledge, there are no definite descriptions on the features and application scopes of these techniques. The survival rates of different in vitro culture methods were compared and the EGFP expression areas of different gene transfer techniques were explored. It was that the optimal timings of removing embryo for EC culture and Petri dish system was at E1.5 and E2.5, respectively; and optimal timing of injecting retrovirus is at E0. Results indicated that the EC culture, in ovo electroporation, the Petri dish system and retrovirus-mediated method are, respectively, suitable for the very early, early, late and whole embryonic stages in chicken. Comparison of different gene transfer methods and establishment of optimal timings are expected to provide a better choice of the efficient method for a particular experiment.
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, 430070, Wuhan, Hubei, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Gong
authors.forename: Y P
authors.initials: YP
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.forename: Yan P
authors.initials: YP
authors.lastname: Feng
authors.forename: S J
authors.initials: SJ
authors.lastname: Li
authors.forename: Xiu L
authors.initials: XL
authors.lastname: Peng
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Gong
mesh_terms: D000818:Animals; D002642:Chick Embryo; D018274:Electroporation; D046149:Embryo Culture Techniques; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D007267:Injections; D012190:Retroviridae / Q000235:genetics*; D013997:Time Factors
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins