title: Topical administration of peroxiredoxin-6 on the cornea suppresses inflammation and neovascularization induced by ultraviolet radiation.
abstract: OBJECTIVE
  To investigate the effect of topical administration of peroxiredoxin-6 (PRDX6) on ultraviolet-induced corneal injury.
  
  
  METHODS
  Corneal transparency and neovascularization were observed with a slit-lamp microscope and hematoxylin and eosin staining. The oxidative damage was determined with a commercial malondialdehyde (MDA) kit. The expressions of PRDX6, polymorphonuclear neutrophil (PMN), vascular endothelial growth factor (VEGF), and pigment epithelium-derived factor (PEDF) were determined by immunohistochemistry and Western blot. The expressions of genes related with antioxidant defense systems and cell apoptosis were detected by RT-PCR.
  
  
  RESULTS
  The irradiated corneas appeared opaque and had high levels of MDA. Peripheral neovascularization and neutrophils appeared in the control and buffer-treated groups (with no treatment or PRDX6 diluent, respectively), whereas they were significantly suppressed in the PRDX6-treated group. The MDA content of the corneas in the PRDX6-treated group was significantly lower than that of the control and buffer-treated groups (P < 0.05). In the PRDX6-treated group the immunoreactivity of VEGF was lower, and that of PEDF was higher, than that in the control and buffer-treated groups. In addition, there were expression correlations between PRDX6 and PMN, VEGF, PEDF. The expressions of genes related with antioxidant defense systems and cell apoptosis were significant different between buffer- and PRDX6-treated groups (P < 0.05).
  
  
  CONCLUSIONS
  The topically administered PRDX6 maintained the homeostasis of corneal cells, reduced inflammation, and suppressed neovascularization and apoptosis under ultraviolet irradiation.
authors.affiliation: Institute of Reproductive and Developmental Biology, College of Life Science, Yantai University, Yantai, Shandong Province, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Shi
authors.forename: Hua Jun
authors.initials: HJ
authors.lastname: Yu
authors.forename: Hai Yan
authors.initials: HY
authors.lastname: Wang
authors.forename: Wen Ting
authors.initials: WT
authors.lastname: Wang
authors.forename: Shao Hua
authors.initials: SH
authors.lastname: Jin
authors.forename: Peng
authors.initials: P
authors.lastname: Zhu
authors.forename: Shi Jia
authors.initials: SJ
authors.lastname: Li
authors.forename: Cheng Ting
authors.initials: CT
authors.lastname: Rong
authors.forename: Jian Yuan
authors.initials: JY
authors.lastname: Li
mesh_terms: D000287:Administration, Topical; D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D003315:Cornea / Q000187:drug effects* / Q000528:radiation effects; D016510:Corneal Neovascularization / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D003318:Corneal Opacity / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism; D007124:Immunoenzyme Techniques; D007634:Keratitis / Q000209:etiology / Q000276:immunology / Q000517:prevention & control*; D008297:Male; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism; D009504:Neutrophils / Q000276:immunology; D054465:Peroxiredoxin VI / Q000008:administration & dosage* / Q000378:metabolism; D011833:Radiation Injuries, Experimental / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015843:Serpins / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism; D000099174:Pigment Epithelium-Derived Factor
chemical_list: D016207:Cytokines; D005136:Eye Proteins; D009414:Nerve Growth Factors; D054465:Peroxiredoxin VI; D011994:Recombinant Proteins; D015843:Serpins; D042461:Vascular Endothelial Growth Factor A; D000099174:Pigment Epithelium-Derived Factor; C467498:vascular endothelial growth factor A, rat