title: [Isolation and purification of human blood plasma proteins able to form potassium channels in artificial bilayer lipid membrane].
abstract: Protein fraction able to induce K(+)-selective transport across bilayer lipid membrane was isolated from human blood plasma with the use of the detergent and proteolytic enzyme-free method developed at our laboratory. After addition of the studied sample to the artificial membrane in the presence of 100 mM KCl, a discrete current change was observed. No channel activity was recorded in the presence of calcium and sodium ions. Channel forming activity of fraction was observed only in the presence of K+. Using a threefold gradient of KCl in the presence of studied proteins the potassium-selective potential balanced by voltage of -29 mV was registered. This value is very close to the theoretical Nernst potential in this case. This means that the examined ion channel is cation-selective. According to data obtained with MS-MALDI-TOF/TOF and database NCBI three protein components were identified in isolated researched sample.
authors.forename: N I
authors.initials: NI
authors.lastname: Venediktova
authors.forename: K V
authors.initials: KV
authors.lastname: Kuznetsov
authors.forename: E N
authors.initials: EN
authors.lastname: Gritsenko
authors.forename: G P
authors.initials: GP
authors.lastname: Gulidova
authors.forename: G D
authors.initials: GD
authors.lastname: Mironova
mesh_terms: D000595:Amino Acid Sequence; D016632:Apolipoprotein A-I / Q000737:chemistry* / Q000378:metabolism; D001692:Biological Transport; D001798:Blood Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002308:Cardiolipins / Q000737:chemistry*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004553:Electric Conductivity; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D008564:Membrane Potentials; D008969:Molecular Sequence Data; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000737:chemistry* / Q000378:metabolism; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D016632:Apolipoprotein A-I; D001798:Blood Proteins; D002308:Cardiolipins; D008051:Lipid Bilayers; D015221:Potassium Channels; D012709:Serum Albumin; D012964:Sodium; D011188:Potassium; D002118:Calcium