title: Engagement of new castle disease virus (NDV) matrix (M) protein with charged multivesicular body protein (CHMP) 4 facilitates viral replication.
abstract: Newcastle disease virus (NDV) causes heavy economic losses to poultry industry across the globe every year. Although NDV matrix (M) protein is involved in virus budding and our previous data indicate that in ovo expression of M protein facilitates NDV replication, the underlying mechanism for the role of M protein in NDV replication is not clear. Using yeast two-hybrid system and immunoprecipitation approaches, we found that M protein interacted with host vacuolar sorting protein charged multivesicular body protein (CHMP) 4B and 4C. In addition, the colocalization of M protein and CHMP4B/C could be observed using a laser confocal scanning microscope. Knockdown of CHMP4B by siRNA or transient expression of CHMP4B/C dominant negative forms markedly inhibited NDV growth in DF-1 cells. Thus, cellular CHMP4s play a critical role in NDV replication.
authors.affiliation: State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Cao
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Shijun J
authors.initials: SJ
authors.lastname: Zheng
mesh_terms: D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D015870:Gene Expression; D020868:Gene Silencing; D057809:HEK293 Cells; D006801:Humans; D057146:Multivesicular Bodies / Q000378:metabolism*; D009522:Newcastle disease virus / Q000254:growth & development / Q000502:physiology*; D011485:Protein Binding; D034741:RNA, Small Interfering; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D034741:RNA, Small Interfering; D014763:Viral Matrix Proteins