title: Epigenetic reprogramming in somatic cells induced by extract from germinal vesicle stage pig oocytes.
abstract: Genomic reprogramming factors in the cytoplasm of germinal vesicle (GV) stage oocytes have been shown to improve the efficiency of producing cloned mouse offspring through the exposure of nuclei to a GV cytoplasmic extract prior to somatic cell nuclear transfer (SCNT) to enucleated oocytes. Here, we developed an extract of GV stage pig oocytes (GVcyto-extract) to investigate epigenetic reprogramming events in treated somatic cell nuclei. This extract induced differentiation-associated changes in fibroblasts, resulting in cells that exhibit pluripotent stem cell-like characteristics and that redifferentiate into three primary germ cell layers both in vivo and in vitro. The GVcyto-extract treatment induced large numbers of high-quality SCNT-generated blastocysts, with methylation and acetylation of H3-K9 and expression of Oct4 and Nanog at levels similar to in vitro fertilized embryos. Thus, GVcyto-extract could elicit differentiation plasticity in treated fibroblasts, and SCNT-mediated reprogramming reset the epigenetic state in treated cells more efficiently than in untreated cells. In summary, we provide evidence for the generation of stem-like cells from differentiated somatic cells by treatment with porcine GVcyto-extract.
authors.affiliation: Department of Animal Biotechnology, College of Animal Bioscience and Biotechnology/Animal Resources Research Center, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Korea. bthuy@konkuk.ac.kr
authors.forename: Hong-Thuy
authors.initials: HT
authors.lastname: Bui
authors.forename: Deug-Nam
authors.initials: DN
authors.lastname: Kwon
authors.forename: Min-Hui
authors.initials: MH
authors.lastname: Kang
authors.forename: Mi-Hye
authors.initials: MH
authors.lastname: Oh
authors.forename: Mi-Ryung
authors.initials: MR
authors.lastname: Park
authors.forename: Woo-Jin
authors.initials: WJ
authors.lastname: Park
authors.forename: Seung-Sam
authors.initials: SS
authors.lastname: Paik
authors.forename: Nguyen
authors.initials: N
authors.lastname: Van Thuan
authors.forename: Jin-Hoi
authors.initials: JH
authors.lastname: Kim
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002457:Cell Extracts / Q000494:pharmacology*; D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D065150:Cellular Reprogramming; D044127:Epigenesis, Genetic; D005347:Fibroblasts / Q000187:drug effects; D006657:Histones / Q000378:metabolism; D018398:Homeodomain Proteins / Q000096:biosynthesis; D057026:Induced Pluripotent Stem Cells; D008745:Methylation / Q000187:drug effects; D053652:Nuclear Transfer Techniques; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis / Q000235:genetics; D009865:Oocytes; D039904:Pluripotent Stem Cells; D013552:Swine
chemical_list: D002457:Cell Extracts; D006657:Histones; D018398:Homeodomain Proteins; D050814:Octamer Transcription Factor-3