title: Effects of etch-and-rinse and self-etch adhesives on dentin MMP-2 and MMP-9.
abstract: Auto-degradation of collagen matrices occurs within hybrid layers created by contemporary dentin bonding systems, by the slow action of host-derived matrix metalloproteinases (MMPs). This study tested the null hypothesis that there are no differences in the activities of MMP-2 and -9 after treatment with different etch-and-rinse or self-etch adhesives. Tested adhesives were: Adper Scotchbond 1XT (3M ESPE), PQ1 (Ultradent), Peak LC (Ultradent), Optibond Solo Plus (Kerr), Prime&Bond NT (Dentsply) (all 2-step etch-and-rinse adhesives), and Adper Easy Bond (3M ESPE), Tri-S (Kuraray), and Xeno-V (Dentsply) (1-step self-etch adhesives). MMP-2 and -9 activities were quantified in adhesive-treated dentin powder by means of an activity assay and gelatin zymography. MMP-2 and MMP-9 activities were found after treatment with all of the simplified etch-and-rinse and self-etch adhesives; however, the activation was adhesive-dependent. It is concluded that all two-step etch-and-rinse and the one-step self-etch adhesives tested can activate endogenous MMP-2 and MMP-9 in human dentin. These results support the role of endogenous MMPs in the degradation of hybrid layers created by these adhesives.
authors.affiliation: Department of Biomedicine, Unit of Dental Sciences and Biomaterials, University of Trieste, Trieste, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Mazzoni
authors.forename: P
authors.initials: P
authors.lastname: Scaffa
authors.forename: M
authors.initials: M
authors.lastname: Carrilho
authors.forename: L
authors.initials: L
authors.lastname: Tjäderhane
authors.forename: R
authors.initials: R
authors.lastname: Di Lenarda
authors.forename: A
authors.initials: A
authors.lastname: Polimeni
authors.forename: A
authors.initials: A
authors.lastname: Tezvergil-Mutluay
authors.forename: F R
authors.initials: FR
authors.lastname: Tay
authors.forename: D H
authors.initials: DH
authors.lastname: Pashley
authors.forename: L
authors.initials: L
authors.lastname: Breschi
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods*; D003738:Dental Cements / Q000494:pharmacology; D003804:Dentin / Q000187:drug effects* / Q000201:enzymology; D017220:Dentin-Bonding Agents / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D020536:Enzyme Activators / Q000494:pharmacology; D006801:Humans; D008422:Materials Testing; D020778:Matrix Metalloproteinase 2 / Q000187:drug effects*; D020780:Matrix Metalloproteinase 9 / Q000187:drug effects*; D010756:Phosphoric Acids / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000494:pharmacology; D019279:Resin Cements / Q000494:pharmacology
chemical_list: C515604:Clearfil Tri-S Bond; D003738:Dental Cements; D017220:Dentin-Bonding Agents; D020536:Enzyme Activators; C451600:OptiBond Solo Plus; C119199:PermaQuik; D010756:Phosphoric Acids; D011109:Polymethacrylic Acids; C425843:Prime and Bond NT; D019279:Resin Cements; C575872:Xeno V; C041330:Scotchbond; C030242:phosphoric acid; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9