title: Detection of a bocavirus circular genome in fecal specimens from children with acute diarrhea in Beijing, China.
abstract: To determine if human bocavirus 2 (HBoV2) has a circular genome similar to the head-to-tail sequence of HBoV1 and the episomal form of HBoV3, 15 HBoV2 positive samples identified from 553 stool specimens from children with acute diarrhea were tested for a head-to-tail sequence using TaqMan-based real-time PCR. A circular genome with a head-to-tail sequence was identified in one (BJQ435) out of 15 samples tested by nested PCR. The complete circular genome of HBoV2-C1 (BJQ435) was 5307 nt in length and was flanked with a 520 nt-long terminal non-coding region (NCR). The secondary structure of HBoV2 -C1 had some differences compared to HBoV3-E1 (JN086998). Our study indicates that the HBoV genome exists in the form of a head-to-tail monomer and provides more information for understanding the HBoV replication mechanism.
authors.affiliation: Graduate School of Peking Union Medical College, Beijing, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.forename: Linqing
authors.initials: L
authors.lastname: Zhao
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.forename: Yuan
authors.initials: Y
authors.lastname: Qian
authors.forename: Liying
authors.initials: L
authors.lastname: Liu
authors.forename: Liping
authors.initials: L
authors.lastname: Jia
authors.forename: You
authors.initials: Y
authors.lastname: Zhang
authors.forename: Huijin
authors.initials: H
authors.lastname: Dong
mesh_terms: D000293:Adolescent; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D002681:China; D004279:DNA, Viral / Q000032:analysis; D003967:Diarrhea / Q000503:physiopathology* / Q000821:virology*; D005243:Feces; D005260:Female; D005759:Gastroenteritis / Q000235:genetics / Q000821:virology; D016679:Genome, Viral; D056661:Human bocavirus / Q000378:metabolism*; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D015995:Prevalence; D017433:Protein Structure, Secondary; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004279:DNA, Viral