title: HPLC-UV-ESI-MS analysis of phenolic compounds and antioxidant properties of Hypericum undulatum shoot cultures and wild-growing plants.
abstract: LC-UV and LC-MS analysis were used to study the phenolic composition of water extracts of Hypericum undulatum (HU) shoot cultures and wild-growing (WG) plants. Total phenolic content (TPC), determined using the Folin-Ciocalteu assay, and the antioxidant activity measured by two complementary methods were also performed for each sample. Mass spectrometry revealed several phenolics acids with quinic acid moieties, flavonols, mostly quercetin, luteolin and apigenin glycosides, flavan-3-ols (catechin and epicatechin) and the xanthonoid mangiferin. Differences in phenolic composition profile and TPC were found between the samples. The major phenolic in HU culture-growing (CG) samples is chlorogenic acid, followed by epicatechin, quercitrin and isoquercitrin. The WG plants presents hyperoside as the main phenolic, followed by isoquercitrin, chlorogenic acid and quercetin. The TPC and antioxidant activity were higher in samples from WG plants.
authors.affiliation: Department of Technological Sciences and Development (DCTD), University of Azores, Ponta Delgada, Portugal.
authors.forename: Nuno
authors.initials: N
authors.lastname: Rainha
authors.forename: Kamila
authors.initials: K
authors.lastname: Koci
authors.forename: Ana Varela
authors.initials: AV
authors.lastname: Coelho
authors.forename: Elisabete
authors.initials: E
authors.lastname: Lima
authors.forename: José
authors.initials: J
authors.lastname: Baptista
authors.forename: Manuel
authors.initials: M
authors.lastname: Fernandes-Ferreira
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016166:Free Radical Scavengers / Q000737:chemistry; D020902:Hypericum / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D018520:Plant Shoots / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D010636:Phenols