title: Evaluation of lipid peroxidation and antioxidant system in healthy iron-replete infants receiving iron prophylaxis.
abstract: OBJECTIVE
  Iron supplementation is commonly recommended for infants; however, there are some reports that it causes oxidative damage. The aim of this study was to investigate the potential effects of iron supplementation at 4 mo of age, for a period of 2 mo, on lipid peroxidation and free radical scavenging enzymes.
  
  
  METHODS
  Twenty-seven healthy 4-mo-old infants chosen randomly and given iron supplementation (ferrous sulfate, 10 mg of elemental iron per day) constituted the study group and 26 healthy 4-mo-old infants who were chosen randomly and not given iron supplementation constituted the control group. Weight, height, head circumference, complete blood cell count, serum ferritin level and intraerythrocytic zinc, iron, copper, malondialdehyde, catalase, superoxide dismutase, and glutathione peroxidase levels were measured in the two groups at 4 and 6 mo of life.
  
  
  RESULTS
  Compared with controls at 6 mo of age, no significant differences were observed for intraerythrocytic zinc (0.5 ± 0.1 versus 0.6 ± 0.2 μg/mL, P > 0.05), copper (0.2 ± 0.1 versus 0.2 ± 0.2 μg/mL, P > 0.05), iron (130.8 ± 10.9 versus 127.4 ± 11.1 μg/mL, P > 0.05), malondialdehyde (21.4 ± 3.5 versus 22.4 ± 2.3 nmol/g of hemoglobin, P > 0.05), catalase (135.4 ± 23.9 versus 135.1 ± 23.3 MU/g of hemoglobin, P > 0.05), superoxide dismutase (1736.4 ± 141.1 versus 1701.3 ± 103.9 U/g of hemoglobin, P > 0.05), and glutathione peroxidase (8.9 ± 1.6 versus 8.4 ± 1.6 U/g of hemoglobin, P > 0.05) levels.
  
  
  CONCLUSIONS
  Our study indicates that the supplemental use of elemental iron 10 mg/d for a period of 2 mo in healthy iron-replete infants did not cause lipid peroxidation or an impairment of antioxidant status.
authors.affiliation: Department of Pediatric Nephrology, Gulhane Military Medical Academy and Medical Faculty, Ankara, Turkey.
authors.forename: Duygu Övunc
authors.initials: DÖ
authors.lastname: Hacihamdioglu
authors.forename: A Emin
authors.initials: AE
authors.lastname: Kurekci
authors.forename: Orhan
authors.initials: O
authors.lastname: Gursel
authors.forename: A Avni
authors.initials: AA
authors.lastname: Atay
authors.forename: Necati
authors.initials: N
authors.lastname: Balamtekin
authors.forename: Ahmet
authors.initials: A
authors.lastname: Aydin
authors.forename: Adnan
authors.initials: A
authors.lastname: Haşimi
authors.forename: Okan
authors.initials: O
authors.lastname: Ozcan
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000097:blood; D003300:Copper / Q000097:blood; D019587:Dietary Supplements / Q000009:adverse effects; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005293:Ferritins / Q000097:blood; D005296:Ferrous Compounds / Q000008:administration & dosage / Q000009:adverse effects; D016166:Free Radical Scavengers / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D007223:Infant; D007501:Iron / Q000097:blood; D000090463:Iron Deficiencies; D019266:Iron, Dietary / Q000008:administration & dosage / Q000009:adverse effects*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D013482:Superoxide Dismutase / Q000097:blood; D015032:Zinc / Q000097:blood
chemical_list: D000975:Antioxidants; D005296:Ferrous Compounds; D016166:Free Radical Scavengers; D019266:Iron, Dietary; C020748:ferrous sulfate; D003300:Copper; D005293:Ferritins; D007501:Iron; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D015032:Zinc