title: Isolation of a novel herpesvirus from a Pacific white-sided dolphin.
abstract: During establishment of primary cell culture from the kidney of a dead Pacific white-sided dolphin (Lagenorhynchus obliquidens), a cytopathic effect was observed. Polymerase chain reaction with a set of herpesvirus consensus primers yielded a fragment of the expected size. Nucleotide sequencing of the product indicated that the isolated virus was closely related to an alphaherpesvirus detected in a bottlenose dolphin in the United States, but the sequence identity at the protein level was low (86.6 %). Phylogenetic analysis of the encoded sequence confirmed that the new isolate belonged to the subfamily Alphaherpesvirinae and clustered together with other cetacean alphaherpesviruses. The complete gene encoding glycoprotein B (2,757 bp) was amplified from the novel isolate; the encoded protein was compared with the corresponding protein of other herpesviruses, revealing that this virus belongs to the genus Varicellovirus. Taken together, these results suggest that this virus corresponds to a novel herpesvirus capable of infecting Pacific white-sided dolphins.
authors.affiliation: Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Keita
authors.initials: K
authors.lastname: Noguchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimoda
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Terada
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shimojima
authors.forename: Kaoru
authors.initials: K
authors.lastname: Kohyama
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Inoshima
authors.forename: Ken
authors.initials: K
authors.lastname: Maeda
mesh_terms: D018138:Alphaherpesvirinae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D004289:Dolphins / Q000821:virology*; D006023:Glycoproteins / Q000235:genetics; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D006023:Glycoproteins; D014759:Viral Envelope Proteins