title: Modulation of IgE immunoreactivity to broad bean proteins after food processing in a Moroccan population.
abstract: BACKGROUND
  The aim of this study was to assess the sensitivity profile of the population of Fez and Casablanca in Morocco to dry broad bean (Vicia faba), and to investigate the effect of food processing (heat and/or enzymatic hydrolysis by pepsin) on the human IgE binding capacity to broad bean proteins (BBP).
  
  
  METHODS
  Sera samples from 146 patients with atopic hypersensitivity were recruited in order to evaluate specific IgE levels to native and processed broad bean proteins by ELISA. Under the same conditions, we assessed the immunoreactivity of rabbit IgG obtained by immunisation with native BBP.
  
  
  RESULTS
  High IgE levels to BBP were found; in fact, 79.3% of children and 80.4% of adults had positive values. The heat treatment (70°C during 60 min) of dry beans proteins showed slight reduction in recognition of these antigens by rabbit IgG (22%) and by human IgE (12%). Pepsin hydrolysis decreased rabbit-IgG recognition by 55% in the first 30 min of treatment. In contrast, and under the same conditions, pepsin increased human-IgE recognition with an average of 143% for all patients. However, the combination of the two treatments (heating and pepsin digestion) showed a decrease of 16% in BBP recognition for all patients.
  
  
  CONCLUSIONS
  This study demonstrates a high sensitivity of a Moroccan population to broad bean proteins which was resistant to heat and digestion by pepsin.
authors.affiliation: Laboratory of Bioactive Molecules (LMBSF), Faculty of Sciences & Techniques, Unit: Immunology and pharmacology, P.O. Box: 2202, Immouzer Road, Fez, Morocco. Electronic address: amalben3@yahoo.fr.
authors.forename: A
authors.initials: A
authors.lastname: Bousfiha
authors.affiliation: Laboratory of Bioactive Molecules (LMBSF), Faculty of Sciences & Techniques, Unit: Immunology and pharmacology, P.O. Box: 2202, Immouzer Road, Fez, Morocco.
authors.forename: L
authors.initials: L
authors.lastname: Aarab
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology / Q000378:metabolism*; D052179:Antigens, Plant / Q000276:immunology / Q000378:metabolism*; D002648:Child; D000939:Epitopes / Q000737:chemistry; D005260:Female; D005511:Food Handling; D006358:Hot Temperature; D006801:Humans; D006868:Hydrolysis; D006969:Hypersensitivity, Immediate / Q000175:diagnosis* / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D007223:Infant; D008297:Male; D008875:Middle Aged; D009018:Morocco; D010434:Pepsin A / Q000378:metabolism; D011485:Protein Binding; D012680:Sensitivity and Specificity; D031307:Vicia faba / Q000276:immunology; D055815:Young Adult
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D000939:Epitopes; D007073:Immunoglobulin E; D010434:Pepsin A
keywords: Dry broad bean; Enzymatic hydrolysis; Food hypersensitivity; Heat treatment; Specific IgE