title: [In vitro embryo culture of Epimedium wushanense].
abstract: OBJECTIVE
  To study the in vitro embryo culture of Epimedium wushanense and provide scientific basis for large scale production of tissue culture.
  
  
  METHODS
  Cullus and buds were induced from embryo of E. wushanense on a MS medium supplemented with different 2,4-D,6-BA, NAA, IBA.
  
  
  RESULTS
  The optimal compositions of medium that induced callus and buds from embryo were the MS medium supplemented with 2,4-D 2 mg x L(-1), IBA 2 mg x L(-1) and NAA 0.5 mg x L(-1) and the MS medium supplemented with IBA 2 mg x L(-1) and 6-BA 0.5 mg x L(-1), respectively. The optimum medium for callus differentiation was MS + 6-BA 1 mg x L(-1) + NAA 0.5 mg x L(-1) + IBA 1 mg x L(-1), and MS +6-BA 1.0 mg x L(-1) + NAA 0.5 mg x L(-1) for shoots proliferation.
  
  
  CONCLUSIONS
  Using embryo as explants, the method of induction and culture of E. wushanense was established by the callus and buds, and the embryo of E. wushanense can be quickly propagated.
authors.affiliation: Institute of Chinese Medicinal, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Haiqin
authors.initials: H
authors.lastname: Zhou
authors.forename: Guosheng
authors.initials: G
authors.lastname: Zhiu
authors.forename: Qiaosheng
authors.initials: Q
authors.lastname: Guo
authors.forename: Zuoyi
authors.initials: Z
authors.lastname: Liu
authors.forename: Ning
authors.initials: N
authors.lastname: Zhou
mesh_terms: D003470:Culture Media; D034181:Epimedium / Q000196:embryology*; D012038:Regeneration; D046509:Tissue Culture Techniques
chemical_list: D003470:Culture Media