title: Phenotypic variation caused by variation in the relative copy number of pDU1-based plasmids expressing the GAF domain of Pkn41 or Pkn42 in Anabaena sp. PCC 7120.
abstract: The cyanobacterium Anabaena (Nostoc) sp. PCC 7120 is a model for cyanobacterial cell differentiation studies. pDU1, an endogenous plasmid in Nostoc sp. PCC 7524, is used as the only cyanobacterial replicon for Anabaena (Nostoc) studies. However, the relative copy numbers of pDU1-based plasmids in Anabaena (Nostoc) sp. PCC 7120 are not well studied. We found that the relative plasmid copy number of one such vector, pRL25T, varied widely, especially when the vector carried a recombinant insert, under different conditions, ranging from 0.53 to 1812 per chromosome in different recombinant strains tested, either in independent clones of the same strain or in the same clone under different growth conditions. The phenotypes caused by pRL25T-driven expression of green fluorescent protein or the GAF domain of Pkn41 or Pkn42 varied depending on the independent clones analyzed. This phenotypic variation correlated with the relative plasmid copy number present in cells.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 430070 Wuhan, China. oceany583@yahoo.com.cn
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Xiao-Zhen
authors.initials: XZ
authors.lastname: Huang
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Veronique
authors.initials: V
authors.lastname: Risoul
authors.forename: Cheng-Cai
authors.initials: CC
authors.lastname: Zhang
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Chen
mesh_terms: D017033:Anabaena / Q000201:enzymology* / Q000235:genetics / Q000502:physiology*; D018628:Gene Dosage; D015870:Gene Expression; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D010641:Phenotype; D010957:Plasmids; D011494:Protein Kinases / Q000096:biosynthesis* / Q000235:genetics*
chemical_list: D049452:Green Fluorescent Proteins; D011494:Protein Kinases