title: Denaturation and intermediates study of two sturgeon hemoglobins by n-dodecyl trimethylammonium bromide.
abstract: Varieties of hemoglobin (Hb) forms exist in fish, which are usually well adapted to the different ecological conditions or various habitats. In the current study, Hbs from two Sturgeon species of the Southern Caspian Sea Basin were purified and studied upon interaction with n-dodecyl trimethylammonium bromide (DTAB; as a cationic surfactant) by various methods including UV-visible absorption, dynamic light scattering (DLS), and ANS fluorescence spectrophotometry. The chemometric analysis of Hbs was investigated upon interaction with DTAB under titration, using UV-visible absorption spectra. The chemometric resolution techniques were used to determine the number of the components and mole fraction of the oxidized Hbs. These results provided the evidence for the existence of three different molecular components including native (N), intermediate (I) and denatured (D) in sturgeon Hbs. According to the distribution of intermediates, which were broadened in a range of DTAB concentration, the aggregation states, DLS experiments, and thermal stability (T(m) obtained by differential scanning calorimetry (DSC)), the Acipenser stellatus Hb was more stable compared to Acipenser persicus Hb. These results demonstrate a significant relationship between the stability of fish Hbs and the habitat depth requirements.
authors.affiliation: Department of Marine Biology, Faculty of Marine Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Shohreh
authors.initials: S
authors.lastname: Ariaeenejad
authors.forename: Mehran
authors.initials: M
authors.lastname: Habibi-Rezaei
authors.forename: Kaveh
authors.initials: K
authors.lastname: Kavousi
authors.forename: Shahla
authors.initials: S
authors.lastname: Jamili
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Fatemi
authors.forename: Jun
authors.initials: J
authors.lastname: Hong
authors.forename: Najmeh
authors.initials: N
authors.lastname: Poursasan
authors.forename: Nader
authors.initials: N
authors.lastname: Sheibani
authors.forename: Ali A
authors.initials: AA
authors.lastname: Moosavi-Movahedi
mesh_terms: D000818:Animals; D003902:Detergents / Q000737:chemistry*; D004229:Dithiothreitol / Q000737:chemistry; D017753:Ecosystem; D029941:Fish Proteins / Q000737:chemistry*; D005399:Fishes; D006454:Hemoglobins / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D010084:Oxidation-Reduction; D010316:Particle Size; D011485:Protein Binding; D011489:Protein Denaturation; D055550:Protein Stability; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D019163:Reducing Agents / Q000737:chemistry; D013816:Thermodynamics; D017186:Titrimetry
chemical_list: D003902:Detergents; D029941:Fish Proteins; D006454:Hemoglobins; D000644:Quaternary Ammonium Compounds; D019163:Reducing Agents; C022806:dodecyltrimethylammonium; D004229:Dithiothreitol