title: Drug delivery from hydroxyapatite-coated titanium surfaces using biodegradable particle carriers.
abstract: The goal of this study was to develop a functional titanium (Ti) implant loaded with bioactive molecules using biodegradable polymeric particles as drug delivery carrier for dental applications. In this study, dexamethasone (DEX)-loaded poly(lactic-co-glycolic acid) (PLGA) particles were electrostatically immobilized on a Ti disc surface coated with hydroxyapatite (HA) nanocrystals using a low temperature high speed collision (LTHSC) method. Resorbable blasting media (RBM) Ti discs (S1), HA-Ti discs (S2), and HA-Ti discs treated with DEX-loaded PLGA particles (S3) were fabricated in this study as sample discs. To facilitate surface immobilization, PLGA particles were coated with polyethyleneimine (PEI) to produce a positive surface charge. This modification of PLGA particle surfaces, allowed DEX-loaded PLGA particles to be immobilized on negatively charged S2 disc surface. It was found that DEX-loaded PLGA particles were well dispersed and immobilized onto the S3 disc surfaces. Release profile studies of DEX from S3 discs in a 4-week immersion study indicated an initial burst release followed by sustained release. In vitro evaluation of bone marrow derived mesenchymal stem cells (BMSCs) cultured for 1 and 2 weeks on S3 discs showed greater BMSC differentiation than on S1 or S2 discs, demonstrating that this innovative delivery platform potently induced BMSC differentiation in vitro, and suggesting that it could be exploited for stem cell therapy purposes or to enhance in vivo osteogenesis. In addition, the results of the present study shows that various bioactive molecules that promote bone regeneration can be efficiently incorporated onto HA-Ti surfaces using biodegradable polymeric particles.
authors.affiliation: Institute for Biomaterials Research & Development, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Jun Sik
authors.initials: JS
authors.lastname: Son
authors.forename: Young-Ae
authors.initials: YA
authors.lastname: Choi
authors.forename: Eui-Kyun
authors.initials: EK
authors.lastname: Park
authors.forename: Tae-Yub
authors.initials: TY
authors.lastname: Kwon
authors.forename: Kyo-Han
authors.initials: KH
authors.lastname: Kim
authors.forename: Kyu-Bok
authors.initials: KB
authors.lastname: Lee
mesh_terms: D020341:Absorbable Implants; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003907:Dexamethasone / Q000008:administration & dosage; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D004343:Drug Implants / Q000737:chemistry; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D055672:Static Electricity; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D004337:Drug Carriers; D004343:Drug Implants; D006882:Hydroxyapatites; C098170:hydroxyapatite-titanium alloy; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003907:Dexamethasone; D014025:Titanium