title: Association of eNOS polymorphisms (-786T>C, 4a4b, 894G>T) with colorectal cancer susceptibility in the Korean population.
abstract: BACKGROUND
  Polymorphisms of endothelial nitric oxide synthases (eNOS) have been shown to be associated with cancer susceptibility. However, the results of such studies are conflicting to date. We investigated whether polymorphisms of the eNOS gene correlated with patients with colorectal cancer (CRC), relative to healthy individuals.
  
  
  METHODS
  In the present study, we analyzed three polymorphisms of eNOS (-786T>C, 4a4b, and 894G>T) in 509 healthy controls and 528 patients with CRC. The genotyping of eNOS polymorphisms was performed using polymerase chain reaction or polymerase chain reaction-restriction fragment length polymorphism assays.
  
  
  RESULTS
  We found that the TC+CC genotype of the -786T>C polymorphism was significantly associated with an increased risk of CRC compared with the TT genotype. Similarly, the GT+TT genotype of the 894G>T polymorphism was associated with an increased susceptibility to CRC. However, no evidence was found for any association between the 4a4b polymorphism and CRC risk. In addition, the C/4b/G (-786T>C/4a4b/894G>T) haplotype was significantly associated with increased risk of CRC and C/4b/T (-786T>C/4a4b/894G>T) haplotype was only detected in CRC patients.
  
  
  CONCLUSIONS
  Our study suggests that the eNOS -786T>C and 894G>T polymorphisms may be associated with the development of CRC in the Korean population.
authors.affiliation: Department of Internal Medicine, School of Medicine, CHA University, Seongnam, South Korea.
authors.forename: Moon Ju
authors.initials: MJ
authors.lastname: Jang
authors.forename: Young Joo
authors.initials: YJ
authors.lastname: Jeon
authors.forename: Jong Woo
authors.initials: JW
authors.lastname: Kim
authors.forename: So Young
authors.initials: SY
authors.lastname: Chong
authors.forename: Sung Pyo
authors.initials: SP
authors.lastname: Hong
authors.forename: Doyeun
authors.initials: D
authors.lastname: Oh
authors.forename: Yun Kyung
authors.initials: YK
authors.lastname: Cho
authors.forename: Ki Wha
authors.initials: KW
authors.lastname: Chung
authors.forename: Nam Keun
authors.initials: NK
authors.lastname: Kim
mesh_terms: D000368:Aged; D044466:Asian People; D015179:Colorectal Neoplasms / Q000201:enzymology / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D056910:Republic of Korea
chemical_list: C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III