title: Comparative genomics of neuroglobin reveals its early origins.
abstract: BACKGROUND
  Neuroglobin (Ngb) is a hexacoordinated globin expressed mainly in the central and peripheral nervous system of vertebrates. Although several hypotheses have been put forward regarding the role of neuroglobin, its definite function remains uncertain. Ngb appears to have a neuro-protective role enhancing cell viability under hypoxia and other types of oxidative stress. Ngb is phylogenetically ancient and has a substitution rate nearly four times lower than that of other vertebrate globins, e.g. hemoglobin. Despite its high sequence conservation among vertebrates Ngb seems to be elusive in invertebrates.
  
  
  RESULTS
  We determined candidate orthologs in invertebrates and identified a globin of the placozoan Trichoplax adhaerens that is most likely orthologous to vertebrate Ngb and confirmed the orthologous relationship of the polymeric globin of the sea urchin Strongylocentrotus purpuratus to Ngb. The putative orthologous globin genes are located next to genes orthologous to vertebrate POMT2 similarly to localization of vertebrate Ngb. The shared syntenic position of the globins from Trichoplax, the sea urchin and of vertebrate Ngb strongly suggests that they are orthologous. A search for conserved transcription factor binding sites (TFBSs) in the promoter regions of the Ngb genes of different vertebrates via phylogenetic footprinting revealed several TFBSs, which may contribute to the specific expression of Ngb, whereas a comparative analysis with myoglobin revealed several common TFBSs, suggestive of regulatory mechanisms common to globin genes.
  
  
  CONCLUSIONS
  Identification of the placozoan and echinoderm genes orthologous to vertebrate neuroglobin strongly supports the hypothesis of the early evolutionary origin of this globin, as it shows that neuroglobin was already present in the placozoan-bilaterian last common ancestor. Computational determination of the transcription factor binding sites repertoire provides on the one hand a set of transcriptional factors that are responsible for the specific expression of the Ngb genes and on the other hand a set of factors potentially controlling expression of a couple of different globin genes.
authors.affiliation: Faculty of Medicine, Institute of Bioinformatics, University of Muenster, Muenster, Germany.
authors.forename: Jasmin
authors.initials: J
authors.lastname: Dröge
authors.forename: Amit
authors.initials: A
authors.lastname: Pande
authors.forename: Ella W
authors.initials: EW
authors.lastname: Englander
authors.forename: Wojciech
authors.initials: W
authors.lastname: Makałowski
mesh_terms: D000465:Algorithms; D020816:Amino Acid Motifs; D000818:Animals; D001499:Bayes Theorem; D002470:Cell Survival; D019143:Evolution, Molecular; D005786:Gene Expression Regulation; D023281:Genomics / Q000379:methods; D005914:Globins / Q000235:genetics* / Q000502:physiology*; D006801:Humans; D000860:Hypoxia; D007448:Invertebrates; D016013:Likelihood Functions; D008957:Models, Genetic; D020125:Mutation, Missense; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000502:physiology*; D000078787:Neuroglobin; D018384:Oxidative Stress; D010802:Phylogeny; D054909:Placozoa / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012617:Sea Urchins / Q000378:metabolism*; D017422:Sequence Analysis, DNA
chemical_list: D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D005914:Globins