title: [Association between plasma adiponectin and small, dense low density lipoprotein in patients with coronary artery disease].
abstract: OBJECTIVE
  To observe the association between adiponectin and small dense low-density lipoprotein (sLDL-c) in coronary artery disease (CAD) patients. Furthermore, we sought to determine the association between single nucleotide polymorphisms (SNP) rs1501299 (+276G/T), rs266729 (-11365C/G) and the incidence of CAD.
  
  
  METHODS
  Consecutive subjects with chest discomfort were examined by coronary angiography and divided into non-CAD [n = 250, 147 male, mean age (60.26 ± 7.52) years] and CAD [n = 267, 153 male, mean age (60.79 ± 9.63) years] groups. Blood samples were collected from all participants following an overnight fasting for at least 12 hours. Plasma adiponectin levels were measured by competitive enzyme-linked immunosorbent assay (ELISA). The serum levels of sLDL-C and oxidized low-density lipoprotein (ox-LDL) were determined by ELISA. Genotypes in rs1501299 and rs266729 of the adiponectin were determined by polymerase chain reaction (PCR).
  
  
  RESULTS
  1. The adiponectin levels were significantly lower [(306.17 ± 74.52) mg/L vs. (321.78 ± 86.28) mg/L], whereas sLDL-C and ox-LDL levels were significantly higher [(276.30 ± 45.55) ng/L vs. (249.00 ± 32.02) ng/L and (545.06 ± 115.46) µg/L vs. (497.74 ± 106.09) µg/L, P < 0.05] in CAD group than non-CAD group. 2. Adiponectin level was negatively associated with sLDL-C, whereas sLDL-C positively correlated with ox-LDL in all subjects. 3. Genotype distribution and allele frequencies of rs1501299 and rs266729 were similar between CAD and non-CAD subjects and not related to the serum levels of adiponectin, sLDL-C and ox-LDL.
  
  
  CONCLUSIONS
  Reduced adiponectin and increased sLDL-C were independent risk factors for coronary artery disease. Genetic polymorphisms in rs1501299 and rs266729 were not linked with coronary artery disease.
authors.affiliation: Department of Cardiology, Tianjin Chest Hospital, Tianjin, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Qin
authors.initials: Q
authors.lastname: Qin
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Bei
authors.initials: B
authors.lastname: Ren
authors.forename: Ya-ru
authors.initials: YR
authors.lastname: Lu
authors.forename: Lu
authors.initials: L
authors.lastname: Kou
authors.forename: Ning
authors.initials: N
authors.lastname: Yang
authors.forename: Jin-ping
authors.initials: JP
authors.lastname: Feng
authors.forename: Bing-rang
authors.initials: BR
authors.lastname: Zhao
mesh_terms: D052242:Adiponectin / Q000097:blood* / Q000235:genetics; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003324:Coronary Artery Disease / Q000097:blood* / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: D052242:Adiponectin; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein