title: [Attachment and spreading of fibroblasts on titanium coatings by different micro-arc oxidation time].
abstract: OBJECTIVE
  To investigate the attachment and spreading of fibroblasts on titanium coatings by different micro-arc oxidation(MAO) time.
  
  
  METHODS
  Micro-arc oxidation film was formed on the titanium surface by using micro-arc oxidation. The film morphology and the cell shape on the samples were observed by scanning electron microscopy (SEM) and the surface roughness of film with different time was analyzed by surface coarseness profiling instrument. SPSS11.5 software package was used to analyze the data.
  
  
  RESULTS
  The experiment result showed, the coatings were rough and porous, the longer oxidation time, the larger diameter of porous but the less porous numbers. As the treatment time increased, the surface roughness of micro-arc oxidation specimens increased (P<0.05). Compared with titanium, fibroblasts on titanium MAO coatings had more abundant spreading and adhesion. At 24 h, 48 h and 72 h, the cell number on coatings adhesion increased, but with different oxidation time, the cell shape, number and adhesion weren't obviously changed.
  
  
  CONCLUSIONS
  The results indicate that microarc oxidation increases the surface roughness, have a good influence on cell adhesion and spreading.
authors.affiliation: Department of Stomatology, Qingdao University Hospital. Qingdao, China. laymanmail@163.com
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Feng-ying
authors.initials: FY
authors.lastname: Yan
authors.forename: Jian-zhi
authors.initials: JZ
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
mesh_terms: D002448:Cell Adhesion; D005347:Fibroblasts; D008855:Microscopy, Electron, Scanning; D010084:Oxidation-Reduction; D014025:Titanium
chemical_list: D014025:Titanium