title: Fetal soft tissue examinations by microdissection.
abstract: This chapter describes methods for the examination of fetal abdominal and thoracic soft tissues by microdissection on either fresh (non-rodent) or fixed (rodent) specimens in order to detect structural abnormalities. With hundreds of fetuses examined for each species (rodent and non-rodent) in regulatory reproductive toxicity assessments (ICH, http://www.ich.org/fileadmin/Public_Web_Site/ICH_Products/Guidelines/Multidisciplinary/M3_R2/Step4/M3_R2__Guideline.pdf, 2009; ICH, http://www.ich.org/fileadmin/Public_Web_Site/ICH_Products/Guidelines/Safety/S5_R2/Step4/S5_R2__Guideline.pdf, 2005), microdissection techniques allow a thorough and relatively rapid examination of fetuses for soft tissue abnormalities.
authors.affiliation: Ricerca Biosciences, Saint-Germain sur l'Arbresle, France. Mariline.leroy@ricerca.com
authors.forename: Mariline
authors.initials: M
authors.lastname: Leroy
authors.forename: Audrey
authors.initials: A
authors.lastname: Jocteur-Monrozier
mesh_terms: D000818:Animals; D002585:Cesarean Section; D004626:Embryology / Q000379:methods*; D005260:Female; D005333:Fetus / Q000166:cytology* / Q000196:embryology*; D051379:Mice; D042282:Microdissection / Q000379:methods*; D011247:Pregnancy; D011817:Rabbits; D051381:Rats; D016707:Tissue Fixation