title: [Effect of XPA expression on the chemotherapy sensitivity of A549/DDP cells].
abstract: OBJECTIVE
  To investigate the influence on platinum-based chemotherapy sensitivity by silencing xeroderma pigmentosum group A (XPA) gene expression in non-small cell lung cancer (NSCLC) drug resistance cell lines (A549/DDP).
  
  
  METHODS
  We detected the expression of XPA in lung normal and tumor tissues by immunohistochemistry,  quantitative real-time PCR (qPCR) and Western blotting. We silenced XPA expression in A549/DDP cells by XPA-shRNA transfection,  and detected the expression of XPA by qPCR and Western blotting.  The cell sensitivity to cisplatin and the apoptosis of A549/DDP cells transfected with XPA-shRNA were determined by MTT assay.
  
  
  RESULTS
  The expression of XPA was higher in NSCLC tissues than that in normal lung tissues. Silencing XPA gene increased the apoptosis and sensitivity of A549/DDP cells to cisplatin.
  
  
  CONCLUSIONS
  Silencing XPA gene can partly reverse the cisplatin resistance in human cisplatin-resistant NSCLC cell line A549/DDP.
authors.affiliation: Department of Pulmonary Medicine, Shandong University, Jinan, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jin-xiang
authors.initials: JX
authors.lastname: Wu
authors.forename: Yu-ping
authors.initials: YP
authors.lastname: Wei
authors.forename: Jun-qing
authors.initials: JQ
authors.lastname: Hao
authors.forename: Shan-ying
authors.initials: SY
authors.lastname: Huang
authors.forename: Liang
authors.initials: L
authors.lastname: Dong
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry / Q000188:drug therapy* / Q000473:pathology; D051760:Xeroderma Pigmentosum Group A Protein / Q000032:analysis / Q000235:genetics / Q000502:physiology*
chemical_list: D000970:Antineoplastic Agents; C497314:XPA protein, human; D051760:Xeroderma Pigmentosum Group A Protein; D002945:Cisplatin