title: Differential impact of IL-10 expression on survival and relapse between HPV16-positive and -negative oral squamous cell carcinomas.
abstract: Human papillomavirus (HPV) is a risk factor in a subset of oropharyngeal cancer; however, the contribution of HPV in the malignancy of oral squamous cell carcinomas (OSCC) is not fully understood in Taiwanese. Herein, 61 patients with no risk factors and 117 patients with one or more risk factors were enrolled in this study. HPV16/18 infection rate in non-smokers, non-drinkers and non-betel quid chewers was higher than their counterparts. The development of HPV-infected cancer has been shown to be associated with interleukin-10 (IL-10) expression. To this end, IL-10 mRNA expression in OSCC tumors was evaluated by real-time RT-PCR. Data showed that HPV-positive patients had higher IL-10 mRNA levels than in HPV-negative patients. Kaplan-Meier and Cox-regression analysis indicated that the prognostic significance of IL-10 mRNA on overall survival and relapse free survival was only observed in HPV-positive OSCC, but not in HPV-negative OSCC. Mechanistically, the elevation of IL-10 by E6 was responsible for increased colony formation and migration capability in OSCC cells. Therefore, we suggest that IL-10 induced by E6 promotes cell growth and migration capability and consequent poor survival and relapse in HPV-positive OSCC.
authors.affiliation: Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan, Republic of China.
authors.forename: Chun-Yi
authors.initials: CY
authors.lastname: Chuang
authors.forename: Wen-Wei
authors.initials: WW
authors.lastname: Sung
authors.forename: Lee
authors.initials: L
authors.lastname: Wang
authors.forename: Wea-Long
authors.initials: WL
authors.lastname: Lin
authors.forename: Kun-Tu
authors.initials: KT
authors.lastname: Yeh
authors.forename: Mao-Chang
authors.initials: MC
authors.lastname: Su
authors.forename: Chun-Han
authors.initials: CH
authors.lastname: Hsin
authors.forename: Shiann-Yann
authors.initials: SY
authors.lastname: Lee
authors.forename: Buor-Chang
authors.initials: BC
authors.lastname: Wu
authors.forename: Ya-Wen
authors.initials: YW
authors.lastname: Cheng
authors.forename: Huei
authors.initials: H
authors.lastname: Lee
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000821:virology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D052161:Human papillomavirus 18 / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000821:virology; D011379:Prognosis; D016016:Proportional Hazards Models; D012008:Recurrence; D012307:Risk Factors; D015996:Survival Rate
chemical_list: C508609:IL10 protein, human; D016753:Interleukin-10