title: [Effect of Panax notoginseng saponins on expression of alpha-secretase mRNA in brains of senescence-accelerated SAMP8 mice].
abstract: OBJECTIVE
  To study the effect of Panax notoginseng saponins (PNS) on expression of alpha-aecretase mRNA in the brains of senescence-accelerated SAMP8 mice.
  
  
  METHODS
  SAMP8 mice were randomly divided into the control group, the PNS high-dosage group (200 mg x kg(-1)), the PNS low-dosage group (100 mg x kg(-1)) and the huperzine A group (0.3 mg x kg(-1)), with eight mice in each group. The control group and each administration group were orally administered with the same volume of double distilled water once for consecutively two months. The expression of alpha-secretase (ADAM 9, ADAM10, ADAM17) mRNA was assayed by real-time fluorescence quantitative polymerase chain reaction (Real-time PCR).
  
  
  RESULTS
  The expression of ADAM9 mRNA in PNS high-dosage group and huperzine A group were significantly higher than that of the control group (P < 0.05). The expression of ADAM10 in the PNS high-dosage group, the PNS low-dosage group and the huperzine A group showed no significant difference from the control group.
  
  
  CONCLUSIONS
  PNS can up-regulate expressions of ADAM9 mRNA and down-regulate expressions of ADAM10 mRNA in the brains of SAMP8 mice.
authors.affiliation: Guangxi Medical University, Nanning 530021, China. hjl0221104@163.com
authors.forename: Jinlan
authors.initials: J
authors.lastname: Huang
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.forename: Dengpan
authors.initials: D
authors.lastname: Wu
authors.forename: Lu
authors.initials: L
authors.lastname: Lu
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Gao
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Zhong
mesh_terms: D051722:ADAM Proteins / Q000235:genetics; D000072197:ADAM10 Protein; D000544:Alzheimer Disease / Q000188:drug therapy* / Q000378:metabolism; D053829:Amyloid Precursor Protein Secretases / Q000235:genetics*; D000818:Animals; D004195:Disease Models, Animal; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D053723:Panax notoginseng; D012333:RNA, Messenger / Q000032:analysis*; D012503:Saponins / Q000494:pharmacology*
chemical_list: D008565:Membrane Proteins; D012333:RNA, Messenger; D012503:Saponins; D053829:Amyloid Precursor Protein Secretases; D051722:ADAM Proteins; C498316:Adam9 protein, mouse; D000072197:ADAM10 Protein; C491234:Adam10 protein, mouse