title: A novel mechanism for the promotion of quercetin glycoside absorption by megalo α-1,6-glucosaccharide in the rat small intestine.
abstract: The presence of an α-1,6-glucosaccharide enhances absorption of water-soluble quercetin glycosides, a mixture of quercetin-3-O-β-d-glucoside (Q3G, 31.8%), mono (23.3%), di (20.3%) and more d-glucose adducts with α-1,4-linkage to a d-glucose moiety of Q3G, in a ligated small intestinal loop of anesthetized rats. We prepared α-1,6-glucosaccharides with different degrees of polymerization (DP) enzymatically and separated them into a megalo-isomaltosaccharide-containing fraction (M-IM, average DP=11.0) and an oligo-isomaltosaccharide-containing fraction (O-IM, average DP=3.6). Luminal injection of either saccharide fraction promoted the absorption of total quercetin-derivatives from the small intestinal segment and this effect was greater for M-IM than O-IM addition. M-IM also increased Q3G, but not the quercetin aglycone, concentration in the water-phase of the luminal contents more strongly than O-IM. The enhancement of Q3G solubilization in the luminal contents may be responsible for the increases in the quercetin glucoside absorption promoted by α-1,6-glucosaccharides, especially that by M-IM. These results suggest that the ingestion of α-1,6-glucosaccharides promotes Q3G bioavailability.
authors.affiliation: Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo 060-8589, Japan.
authors.forename: Aki
authors.initials: A
authors.lastname: Shinoki
authors.forename: Weeranuch
authors.initials: W
authors.lastname: Lang
authors.forename: Charin
authors.initials: C
authors.lastname: Thawornkuno
authors.forename: Hee-Kwon
authors.initials: HK
authors.lastname: Kang
authors.forename: Yuya
authors.initials: Y
authors.lastname: Kumagai
authors.forename: Masayuki
authors.initials: M
authors.lastname: Okuyama
authors.forename: Haruhide
authors.initials: H
authors.lastname: Mori
authors.forename: Atsuo
authors.initials: A
authors.lastname: Kimura
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ishizuka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hara
mesh_terms: D000818:Animals; D001682:Biological Availability; D006027:Glycosides / Q000378:metabolism*; D006801:Humans; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000378:metabolism*; D008297:Male; D009844:Oligosaccharides / Q000378:metabolism*; D011794:Quercetin / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006027:Glycosides; D009844:Oligosaccharides; D011794:Quercetin