title: Correlation between three assay systems for anti-Müllerian hormone (AMH) determination.
abstract: OBJECTIVE
  Analysis of anti-Müllerian hormone (AMH) is becoming of recognized importance in reproductive medicine, but assays are not standardized. We have evaluated the correlation between the new Gen II ELISA kit (Beckman-Coutler) and the older ELISA kits by Immunotech (IOT) and Diagnostic Systems Laboratories (DSL).
  
  
  METHODS
  A total of 56 archived serum samples from patients with subfertility or reproductive endocrine disorders were retrieved and assayed in duplicate using the three AMH ELISA kits . The samples covered a wide range of AMH concentrations (1.9 to 142.5 pmol/L).
  
  
  RESULTS
  We observed good correlations between the new (AMH Gen II) and old AMH assay kits by IOT and DSL (R(2) = 0.971 and 0.930 respectively). The regression equations were AMH (Gen II) = 1.353 × AMH (IOT) + 0.051 and AMH (Gen II) = 1.223 × AMH (DSL) - 1.270 respectively.
  
  
  CONCLUSIONS
  AMH concentrations using the Gen II kit are slightly higher than those from the IOT and DSL kits. Standardization of assay results worldwide is urgently required but this analysis facilitates the interpretation of values obtained historically and in future studies using any of the 3 assays available. Meanwhile, adapting clinical cut-offs from previously published work by direct conversion is not recommended.
authors.affiliation: Department of Obstetrics & Gynaecology, Queen Mary Hospital, The University of Hong Kong, 102 Pokfulam Road, Hong Kong, PR China. raymondli@hku.hk
authors.forename: Hang Wun Raymond
authors.initials: HW
authors.lastname: Li
authors.forename: Ernest Hung Yu
authors.initials: EH
authors.lastname: Ng
authors.forename: Benancy Po Chau
authors.initials: BP
authors.lastname: Wong
authors.forename: Richard A
authors.initials: RA
authors.lastname: Anderson
authors.forename: Pak Chung
authors.initials: PC
authors.lastname: Ho
authors.forename: William Shu Biu
authors.initials: WS
authors.lastname: Yeung
mesh_terms: D054304:Anti-Mullerian Hormone / Q000097:blood / Q000302:isolation & purification*; D001681:Biological Assay / Q000592:standards*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards; D005260:Female; D006801:Humans; D007246:Infertility / Q000097:blood / Q000175:diagnosis*; D008297:Male
chemical_list: D054304:Anti-Mullerian Hormone