title: Morphological cell typing of osteoid clones derived from human bone marrow.
abstract: OBJECTIVE
  Bone Marrow Stem Cells (BMSC) are a 'reservoir' for bone regeneration. BMSC can be studied in vitro by cloning cells which are improperly named colonyforming units of fibroblasts (CFU-f). Thus we decided to study CFU-f organization and morphology to have (A) a parameter by which to compare normal and pathologic conditions and (B) to potentially select the most osteogenic clones.
  
  
  METHODS
  Two hundred and forty bone samples were collected from 109 patients and primary cultures performed.
  
  
  RESULTS
  After two weeks 9 cell types and 6 well organized types of colonies were detectable. Some have alkaline phosphatase (AP) activity.
  
  
  CONCLUSIONS
  These data could be relevant to estimate the potential regeneration of bone.
authors.affiliation: Dept. of Oro-maxillofacial and Plastic Surgery, National Medical University, Kiev, Ukraine.
authors.forename: Olga
authors.initials: O
authors.lastname: Tsilenko
authors.forename: Vera
authors.initials: V
authors.lastname: Astachova
authors.forename: Vladislav
authors.initials: V
authors.lastname: Malanchuk
authors.forename: Francesco
authors.initials: F
authors.lastname: Carinci
keywords: Bone production in vitro system; Clone; Morphology; Stem cell