title: [Effects of heat and cool-producing needling manipulations on rectal temperature and serum endotoxin content in endotoxin-induced heat syndrome rabbits].
abstract: OBJECTIVE
  To observe the effect of traditional manipulations of "Shaoshanhuo" (heat-producing needling) and "Toutianliang"(cool-producing needling) on body temperature and serum endotoxin level in heat syndrome rabbits.
  
  
  METHODS
  Twenty-four Japanese rabbits were randomly divided into control, model, Shaoshanhuo and Toutianliang groups. Heat-syndrome model was established by subcutaneous injection of bacterium coli endotoxin solution (40 microg/mL, 2 mL/kg). Heat-producing and cool-producing needling was applied to bilateral "Quchi" (LI 11) for 5 min, respectively. Rectal temperature was detected by using a thermometer, and serum endotoxin content assayed by using Limulus Ameboyte Lysate kit (luminescence measuring).
  
  
  RESULTS
  In comparison with the control group, both rectal temperature and serum endotoxin levels were increased significantly in the model group (P < 0.01). While compared to the model group, the rectal temperature and serum endotoxin levels were down-regulated considerably in both Shaoshanhuo and Toutianliang groups (P < 0.05, P < 0.01). The effect of the Toutianliang group was obviously superior to that of the Shaoshanhuo group in reducing serum endotoxin content (P < 0.01).
  
  
  CONCLUSIONS
  Both heat-producing needling and cool-producing needling can lower rectal temperature and serum endotoxin levels in heat-syndrome rabbits, and the effect of cool-producing needling is relatively better in reducing endotoxin content.
authors.affiliation: College of Acu-moxibustion and Tuina, Chengdu University of Chinese Medicine, Chengdu 610072, China. 5014296@qq.com
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Zhou
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Yue
authors.initials: Y
authors.lastname: Feng
authors.forename: Shen-Qiao
authors.initials: SQ
authors.lastname: Yang
mesh_terms: D015670:Acupuncture Therapy / Q000295:instrumentation / Q000379:methods*; D000818:Animals; D001831:Body Temperature; D004731:Endotoxins / Q000009:adverse effects* / Q000097:blood; D005260:Female; D018882:Heat Stress Disorders / Q000209:etiology / Q000503:physiopathology / Q000628:therapy*; D006801:Humans; D008297:Male; D009339:Needles; D011817:Rabbits; D012007:Rectum / Q000737:chemistry*
chemical_list: D004731:Endotoxins