title: The high immunogenicity induced by modified sporozoites' malarial peptides depends on their phi (ϕ) and psi (ψ) angles.
abstract: The importance of CSP- and STARP-derived ϕ and ψ dihedral angles in mHABP structure was analysed by (1)H NMR in the search for molecules which can be included as components of a first-line-of-defence Plasmodium falciparum sporozoite multi-epitope vaccine against the most lethal form of human malaria. Most of the aforementioned dihedral angles were left-hand-like polyproline type II (PPII(L)) structures whilst others had right-hand-like α-helix (α(R)), thus allowing mHABPS to fit better into MHCII molecules and thereby form an appropriate pMHCII complex and also establish the H-bonds which stabilise such complex and by this means induce an appropriate immune response. This information has great implications for vaccine development, malaria being one of them.
authors.affiliation: Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia. mepatarr@gmail.com
authors.forename: Manuel E
authors.initials: ME
authors.lastname: Patarroyo
authors.forename: Adriana
authors.initials: A
authors.lastname: Bermudez
authors.forename: Martha P
authors.initials: MP
authors.lastname: Alba
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry* / Q000276:immunology*; D001029:Aotus trivirgatus; D059806:HLA-DR beta-Chains / Q000737:chemistry / Q000276:immunology; D006801:Humans; D017780:Malaria Vaccines / Q000737:chemistry* / Q000276:immunology*; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D010963:Plasmodium falciparum / Q000276:immunology*; D017433:Protein Structure, Secondary; D015800:Protozoan Proteins / Q000737:chemistry* / Q000276:immunology*; D034101:Sporozoites / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan; D059806:HLA-DR beta-Chains; D017780:Malaria Vaccines; D010446:Peptide Fragments; D010455:Peptides; D015800:Protozoan Proteins; C109185:STARP antigen, Plasmodium falciparum; C041595:circumsporozoite protein, Protozoan; C011083:polyproline