title: Vaspin attenuates the apoptosis of human osteoblasts through ERK signaling pathway.
abstract: It has been hypothesized that adipocytokines originating from adipose tissue may have an important role in bone metabolism. Vaspin is a novel adipocytokine isolated from visceral white adipose tissue, which has been reported to have anti-apoptotic effects in vascular endothelial cells. However, to the best of our knowledge there is no information regarding the effects of vaspin on osteoblast apoptosis. This study therefore examined the possible effects of vaspin on apoptosis in human osteoblasts (hOBs). Our study established that vaspin inhibits hOBs apoptosis induced by serum deprivation, as determined by ELISA and TUNEL assays. Western blot analysis revealed that vaspin upregulates the expression of Bcl-2 and downregulates that of Bax in a dose-dependent manner. Vaspin stimulated the phosphorylation of ERK, and pretreatment of hOBs with the ERK inhibitor PD98059 blocked the vaspin-induced activation of ERK, however, vaspin did not stimulate the phosphorylation of p38, JNK or Akt. Vaspin protects hOBs from serum deprivation-induced apoptosis, which may be mediated by activating the MAPK/ERK signaling pathway.
authors.affiliation: Institute of Metabolism and Endocrinology, The Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhu
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Shan
authors.forename: Jie
authors.initials: J
authors.lastname: Shen
authors.forename: Qiu-Hua
authors.initials: QH
authors.lastname: Liang
authors.forename: Rong-Rong
authors.initials: RR
authors.lastname: Cui
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Guan-Ying
authors.initials: GY
authors.lastname: Liu
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Wu
authors.forename: Qiong
authors.initials: Q
authors.lastname: Lu
authors.forename: Hui
authors.initials: H
authors.lastname: Xie
authors.forename: You-Shuo
authors.initials: YS
authors.lastname: Liu
authors.forename: Ling-Qing
authors.initials: LQ
authors.lastname: Yuan
authors.forename: Er-Yuan
authors.initials: EY
authors.lastname: Liao
mesh_terms: D017209:Apoptosis; D002478:Cells, Cultured; D006801:Humans; D020935:MAP Kinase Signaling System; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D015843:Serpins / Q000378:metabolism*; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; C507339:SERPINA12 protein, human; D015843:Serpins; D051028:bcl-2-Associated X Protein; D051057:Proto-Oncogene Proteins c-akt