title: IL-23 and IL-27 Levels in Macrophages Collected from Peripheral Blood of Patients with Healing Vs Non-Healing Form of Cutaneous Leishmaniasis.
abstract: BACKGROUND
  In this study the level of IL-23 and IL-27 produced by macrophages derived from peripheral blood mononuclear cell culture collected from patients with healing or non-healing form of cutaneous leishmaniasis lesion were compared before and after treatment with live Leishmania to explore whether IL-23 or IL-27 plays any role in healing process of cutaneous lesions induced by L. major.
  
  
  METHODS
  Twenty patients resident in Isfahan Province, with healing or non-healing form of cutaneous leishmaniasis lesion caused by Leishmania major participated in this study. In vitro productions of IL-23 and IL-27 by peripheral blood derived macrophages, before and after stimulation with live L. major (MRHO/IR/75/ER) promastigotes were evaluated using ELISA method. Patient with healing form of lesion received no treatment and patient with non-healing form of lesion received at least 2 courses of glucantime.
  
  
  RESULTS
  The mean production of IL-23 and IL-27 from macrophages of patients with healing form of lesion was significantly higher than patients with non-healing form of lesion. The levels of IL-23 and IL-27 in culture supernatants before and after stimulation in healing form of CL was significantly higher than non- healing form of CL (P < 0.001).
  
  
  CONCLUSIONS
  IL-23 and IL-27 might play a role in human leishmaniasis and further studies are needed to understand the role of IL-23 and IL-27 in leishmaniasis.
authors.affiliation: Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Tolouei
authors.forename: K
authors.initials: K
authors.lastname: Ghaedi
authors.forename: A
authors.initials: A
authors.lastname: Khamesipour
authors.forename: M
authors.initials: M
authors.lastname: Akbari
authors.forename: M
authors.initials: M
authors.lastname: Baghaei
authors.forename: Sj
authors.initials: S
authors.lastname: Hasheminia
authors.forename: M
authors.initials: M
authors.lastname: Narimani
authors.forename: Sh
authors.initials: Sh
authors.lastname: Hejazi
keywords: Cutaneous leishmaniasis; IL-23; IL-27; Macrophages; Promastigote