title: In vitro evaluation of cytotoxicity of hyaluronic acid as an extracellular matrix on OFCOL II cells by the MTT assay.
abstract: OBJECTIVE
  To evaluate the cytotoxicity of hyaluronic acid (HA) on a tissue-engineered compound for bone grafting containing osteoblastic cells (OFCOL II), platelet-rich plasma (PRP) with or without thrombin (Thr), and hydroxyapatite (HP) by the MTT assay.
  
  
  METHODS
  Studied groups were formed as follows: (A) Cells + HA + PRP with Thr + hydroxyapatite (HP); (B) Cells + HA + PRP + HP; (C) Cells + HA + HP; (D) Cells + HP; (E) Cells + HA; (F) Cells + PRP with Thr; (G) Cells + PRP; and (H) Pure Dulbecco's modified Eagle's medium (DMEM) with 15% fetal bovine serum. A 2-way ANOVA and Tukey's test were applied for statistical analysis (P < .05).
  
  
  RESULTS
  Results of cell viability for each group were as follows: A: 79%, B: 67%, C: 68%, D: 99%, E: 74%, G: 89%, F: 90%, and Group H: 100%.
  
  
  CONCLUSIONS
  Results suggested a decrease in cell viability in the presence of HA.
authors.affiliation: Private practice, Porto Alegre, Brazil. Electronic address: danielboeckel@yahoo.com.br.
authors.forename: Daniel Gonçalves
authors.initials: DG
authors.lastname: Boeckel
authors.affiliation: Associate Professor, Graduate Program in Dentistry, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, Brazil.
authors.forename: Rosemary Sadami Arai
authors.initials: RS
authors.lastname: Shinkai
authors.affiliation: Associate Professor, Graduate Program in Dentistry, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, Brazil.
authors.forename: Márcio Lima
authors.initials: ML
authors.lastname: Grossi
authors.affiliation: Professor, Graduate Program in Dentistry, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, Brazil.
authors.forename: Eduardo Rolim
authors.initials: ER
authors.lastname: Teixeira
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D017886:Durapatite / Q000494:pharmacology; D005109:Extracellular Matrix / Q000187:drug effects*; D006801:Humans; D006820:Hyaluronic Acid / Q000633:toxicity*; D066298:In Vitro Techniques; D010006:Osteoblasts / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects; D053657:Platelet-Rich Plasma; D013778:Tetrazolium Salts / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry; D013917:Thrombin / Q000494:pharmacology; D023822:Tissue Engineering
chemical_list: D013778:Tetrazolium Salts; D013844:Thiazoles; D006820:Hyaluronic Acid; D017886:Durapatite; D013917:Thrombin