title: The shell-forming proteome of Lottia gigantea reveals both deep conservations and lineage-specific novelties.
abstract: Proteins that are occluded within the molluscan shell, the so-called shell matrix proteins (SMPs), are an assemblage of biomolecules attractive to study for several reasons. They increase the fracture resistance of the shell by several orders of magnitude, determine the polymorph of CaCO(3) deposited, and regulate crystal nucleation, growth initiation and termination. In addition, they are thought to control the shell microstructures. Understanding how these proteins have evolved is also likely to provide deep insight into events that supported the diversification and expansion of metazoan life during the Cambrian radiation 543 million years ago. Here, we present an analysis of SMPs isolated form the CaCO(3) shell of the limpet Lottia gigantea, a gastropod that constructs an aragonitic cross-lamellar shell. We identified 39 SMPs by combining proteomic analysis with genomic and transcriptomic database interrogations. Among these proteins are various low-complexity domain-containing proteins, enzymes such as peroxidases, carbonic anhydrases and chitinases, acidic calcium-binding proteins and protease inhibitors. This list is likely to contain the most abundant SMPs of the shell matrix. It reveals the presence of both highly conserved and lineage-specific biomineralizing proteins. This mosaic evolutionary pattern suggests that there may be an ancestral molluscan SMP set upon which different conchiferan lineages have elaborated to produce the diversity of shell microstructures we observe nowadays.
authors.affiliation: UMR 6282 (ex 5561) CNRS Biogéosciences, Université de Bourgogne, Dijon, France. bmarie@mnhn.fr
authors.forename: Benjamin
authors.initials: B
authors.lastname: Marie
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Jackson
authors.forename: Paula
authors.initials: P
authors.lastname: Ramos-Silva
authors.forename: Isabelle
authors.initials: I
authors.lastname: Zanella-Cléon
authors.forename: Nathalie
authors.initials: N
authors.lastname: Guichard
authors.forename: Frédéric
authors.initials: F
authors.lastname: Marin
mesh_terms: D000595:Amino Acid Sequence; D060105:Animal Shells / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D002256:Carbonic Anhydrases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D021983:Cyclophilins / Q000302:isolation & purification / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004815:Epidermal Growth Factor / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D049851:Gastropoda / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D010544:Peroxidases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D017434:Protein Structure, Tertiary; D020543:Proteome / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D053719:Tandem Mass Spectrometry
chemical_list: D016326:Extracellular Matrix Proteins; D010446:Peptide Fragments; D020543:Proteome; C403490:perlustrin; D004815:Epidermal Growth Factor; D010544:Peroxidases; D006026:Glycoside Hydrolases; D002256:Carbonic Anhydrases; D021983:Cyclophilins