title: The dose-response relationship for dicentric chromosomes and γ-H2AX foci in human peripheral blood lymphocytes: influence of temperature during exposure and intra- and inter-individual variability of donors.
abstract: OBJECTIVE
  Hypothermia during in vitro irradiation of human peripheral blood lymphocytes (PBL) affects the level of chromosome aberrations. The molecular mechanisms of this phenomenon are not fully understood. The aim of our study was to examine the effect of hypothermia on the dose-response relationship for dicentric chromosomes and the level of γ-H2AX (phosphorylated histone H2AX) foci. In addition, the inter- and intra-individual variability was assessed in relation to temperature.
  
  
  METHODS
  PBL were kept at 0.8, 20 and 37°C and then exposed to gamma-rays (from 0-3 Gy). Dicentric chromosomes were scored in first post-treatment mitoses. γ-H2AX foci were scored 15, 30, 60, 120 min and 24 h post irradiation.
  
  
  RESULTS
  Our results revealed that the frequency of dicentric chromosomes in cells exposed at 37°C to gamma-rays was higher than after exposure at 0.8 and 20°C. No effect of temperature was observed on the number of γ-H2AX foci as well as on the intra- and inter-individual variations of the dicentric yield and the number of γ-H2AX foci.
  
  
  CONCLUSIONS
  Temperature at exposure to ionizing radiation has a pronounced effect on the level of cytogenetic damage but not γ-H2AX foci.
authors.affiliation: Department of Radiobiology and Immunology, Institute of Biology, Jan Kochanowski University, Šwiętokrzyska, Kielce, Poland.
authors.forename: Halina
authors.initials: H
authors.lastname: Lisowska
authors.forename: Aneta
authors.initials: A
authors.lastname: Wegierek-Ciuk
authors.forename: Anna
authors.initials: A
authors.lastname: Banasik-Nowak
authors.forename: Janusz
authors.initials: J
authors.lastname: Braziewicz
authors.forename: Maria
authors.initials: M
authors.lastname: Wojewodzka
authors.forename: Andrzej
authors.initials: A
authors.lastname: Wojcik
authors.forename: Anna
authors.initials: A
authors.lastname: Lankoff
mesh_terms: D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays / Q000009:adverse effects; D006657:Histones / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects*; D013696:Temperature
chemical_list: C468783:H2AX protein, human; D006657:Histones