title: Low-level laser therapy using the minimally invasive laser needle system on osteoporotic bone in ovariectomized mice.
abstract: This study tested the effectiveness of low-level laser therapy (LLLT) in preventing and/or treating osteoporotic trabecular bone. Mice were ovariectomized (OVX) to induce osteoporotic bone loss. The tibiae of eight OVX mice were treated for 5 days each week for 2 weeks by LLLT (660 nm, 3 J) using a minimally invasive laser needle system (MILNS) which is designed to minimize loss of laser energy before reaching bone (LASER group). Another eight mice received a sham treatment (SHAM group). Structural parameters of trabecular bone were measured with in vivo micro-computed tomography images before and after laser treatment. After LLLT for 2 weeks, the percentage reduction (%R) was significantly lower in BV/TV (bone volume fraction) and Tb.N (trabecular number, p<0.05 and p<0.05) and significant higher in Tb.Sp (trabecular separation) and SMI (structure model index, p<0.05 and p<0.05) than in the SHAM group. The %R in BV/TV at sites directly treated by LLLT was significantly lower in the LASER group than the SHAM group (p<0.05, p<0.05). These results indicated that LLLT using MILNS may be effective for preventing and/or treating trabecular bone loss and the effect may be site-dependent in the same bone.
authors.affiliation: Department of Biomedical Engineering and Yonsei-Fraunhofer Medical Device Lab, Yonsei University, Wonju, Gangwon 220-710, Republic of Korea.
authors.forename: Chang-Yong
authors.initials: CY
authors.lastname: Ko
authors.forename: Heesung
authors.initials: H
authors.lastname: Kang
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Seo
authors.forename: Byungjo
authors.initials: B
authors.lastname: Jung
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schreiber
authors.forename: Han Sung
authors.initials: HS
authors.lastname: Kim
mesh_terms: D000818:Animals; D005260:Female; D028022:Low-Level Light Therapy / Q000295:instrumentation*; D051379:Mice; D009339:Needles; D010024:Osteoporosis / Q000517:prevention & control* / Q000532:radiotherapy*; D010052:Ovariectomy; D013977:Tibia / Q000528:radiation effects*; D013997:Time Factors