title: Heparinized magnetic mesoporous silica nanoparticles as multifunctional growth factor delivery carriers.
abstract: Well-defined magnetic mesoporous silica nanoparticles (MMSNs) with a core/shell structure were prepared via a one pot synthesis. Sphere-like magnetite aggregates were obtained as cores of the final nanoparticles by assembly in the presence of polyvinyl pyrrolidone and cetyltrimethylammonium bromide. The nanoparticles have the property of superparamagnetism with a saturation magnetization value of 20.3 emu g(-1). In addition, the combination of heparin and fluorescence-labeled MMSNs endows the resultant particles (denoted as MFMSNs-HP) with magnetism and fluorescence properties, excellent dispersity in the buffer solutions and cell culture media, anticoagulant activity in the blood stream, and the controlled release of basic fibroblast growth factor (bFGF). Furthermore, the bFGF cell viability assays indicate that MFMSNs-HP has nearly no toxicity to human umbilical vein endothelial cells (HUVEC) up to a concentration of 200 μg ml(-1), and the proliferation activity of bFGF incorporated into MFMSNs-HP could be retained for at least 6 days. All of these suggest that MFMSNs-HP may serve as a multifunctional carrier for the delivery of growth factors.
authors.affiliation: College of Pharmacy, Key Laboratory of Natural Medicine and Immuno-Engineering of Henan Province, Institute of Environmental Health, Henan University, Kaifeng, People's Republic of China. henuwuqiang@henu.edu.cn
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wu
authors.forename: Chaoqun
authors.initials: C
authors.lastname: Liu
authors.forename: Luna
authors.initials: L
authors.lastname: Fan
authors.forename: Jiahua
authors.initials: J
authors.lastname: Shi
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Liu
authors.forename: Ruifang
authors.initials: R
authors.lastname: Li
authors.forename: Liwei
authors.initials: L
authors.lastname: Sun
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D003692:Delayed-Action Preparations / Q000737:chemistry*; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage* / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000737:chemistry; D006493:Heparin / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D003692:Delayed-Action Preparations; D005456:Fluorescent Dyes; D058185:Magnetite Nanoparticles; D016222:Fibroblast Growth Factor 2; D012822:Silicon Dioxide; D006493:Heparin