title: Does copper stress lead to spindle misposition-dependent cell cycle arrest?
abstract: Because of its specific electrochemical  properties, copper is an essential heavy metal for living organisms. As with  other heavy metals, high levels can provoke damage. We examined gene expression  under copper stress in wild-type fission yeast (Schizosaccharomyces  pombe) through differential display. After the EC(50) concentration of CuSO(4) was determined as 50 μM, total RNA was isolated from cells  treated or not with copper. The expression level of SPCC1682.13, ppk1,  SPBC2F12.05c, and adg2 genes increased significantly under copper stress.  Considering the functions of these genes are related to the cell cycle, cell  division and chromosome dynamics, we hypothesize that retardation of the cell  cycle under copper stress is relevant to the events that depend on the  functions of these genes.
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, Vezneciler, Istanbul, Turkey. cagataytarhan@yahoo.com
authors.forename: C
authors.initials: C
authors.lastname: Tarhan
authors.forename: A T
authors.initials: AT
authors.lastname: Sarikaya
mesh_terms: D001483:Base Sequence; D059447:Cell Cycle Checkpoints / Q000187:drug effects* / Q000235:genetics; D003300:Copper / Q000633:toxicity*; D019327:Copper Sulfate / Q000633:toxicity; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005800:Genes, Fungal / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D012568:Schizosaccharomyces / Q000166:cytology / Q000187:drug effects / Q000235:genetics; D017422:Sequence Analysis, DNA; D008941:Spindle Apparatus / Q000187:drug effects* / Q000378:metabolism*; D013312:Stress, Physiological / Q000187:drug effects* / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D003300:Copper; D019327:Copper Sulfate