title: Qualitative and quantitative simultaneous determination of six marker compounds in Soshiho-tang by HPLC-DAD-ESI-MS.
abstract: Soshiho-tang, one of the herbal prescriptions, has been used in treatment of chronic liver disease. In this study, the efficient high-performance liquid chromatography coupled with diode array detection and electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI-MS) method was developed and validated for simultaneous determination of six marker compounds, namely homogentisic acid, baicalin, glycyrrhizin, saikosaponin A, 6-gingerol and ginsenoside Rg3 in Soshiho-tang. The analysis of six marker compounds was carried out using a C(18) column (SHISEIDO S-5 μm, 4.6 mm I.D. × 250 mm) and gradient elution with water - 0.1% trifluoroacetic acid and acetonitrile. The analytical method was validated for linearity, precision and accuracy. Calibration curve for six marker compounds showed good linear regression (r(2) > 0.9996). The limits of detection (LOD) and limits of quantification (LOQ) for analytes were ranged from 0.04 to 0.16 μg/mL and 0.12 to 0.47 μg/mL, respectively. The relative standard deviations (RSD%) of the precision test, intra- and inter-day, were less than 0.32% and 0.10%, respectively. All recoveries of the six marker compounds were ranged from 100.05% to 102.25% with RSD less than 0.63%. These results have shown that this developed method was efficient for the quality evaluation of Soshiho-tang.
authors.affiliation: TKM Converging Research Division, Korea Institute of Oriental Medicine, Daejeon, Korea.
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Yang
authors.forename: Jin Yeul
authors.initials: JY
authors.lastname: Ma
authors.forename: Jin Bae
authors.initials: JB
authors.lastname: Weon
authors.forename: Bohyoung
authors.initials: B
authors.lastname: Lee
authors.forename: Choong Je
authors.initials: CJ
authors.lastname: Ma
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D055670:Medicine, Korean Traditional; D015394:Molecular Structure; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry* / Q000592:standards; D011208:Powders; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D010936:Plant Extracts; D011208:Powders; C580585:soshiho-tang