title: L-DNA molecular beacon: a safe, stable, and accurate intracellular nano-thermometer for temperature sensing in living cells.
abstract: Noninvasive and accurate measurement of intracellular temperature is of great significance in biology and medicine. This paper describes a safe, stable, and accurate intracellular nano-thermometer based on an L-DNA molecular beacon (L-MB), a dual-labeled hairpin oligonucleotide built from the optical isomer of naturally occurring d-DNA. Relying on the temperature-responsive hairpin structure and the FRET signaling mechanism of MBs, the fluorescence of L-MBs is quenched below the melting temperature and enhanced with increasing temperature. Because of the excellent reversibility and tunable response range, L-MBs are very suitable for temperature sensing. More importantly, the non-natural L-DNA backbone prevents the L-MBs from binding to cellular nucleic acids and proteins as well as from being digested by nucleases inside the cells, thus ensuring excellent stability and accuracy of the nano-thermometer in a complex cellular environment. The L-MB nano-thermometer was used for the photothermal study of Pd nanosheets in living cells, establishing the nano-thermometer as a useful tool for intracellular temperature measurement.
authors.affiliation: State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Ke
authors.forename: Chunming
authors.initials: C
authors.lastname: Wang
authors.forename: Yun
authors.initials: Y
authors.lastname: Ge
authors.forename: Nanfeng
authors.initials: N
authors.lastname: Zheng
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhu
authors.forename: Chaoyong James
authors.initials: CJ
authors.lastname: Yang
mesh_terms: D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D018613:Microscopy, Confocal; D036103:Nanotechnology; D013696:Temperature
chemical_list: D004247:DNA