title: Synthesis and characterization of water-insoluble statistical copolymer and its application in the development of electrochemical DNA sensor.
abstract: Water-insoluble statistical copolymer was synthesized by copolymerization of methyl methacrylate (MMA) with 2-(dimethylamino)ethyl methacrylate (DMA) via group transfer polymerization (GTP). The DMA residues of the precursor P(MMA-co-DMA) statistical copolymer were then quaternized by reacting with methyl iodide under mild conditions to get well-defined P(MMA-co-QDMA) cationic copolymer. Then, P(MMA-co-QDMA) copolymer was successfully used for surface modification of pencil graphite electrode (PGE) to develop a disposable DNA sensor. This P(MMA-co-QDMA) copolymer modified electrode (q-PGE) was examined for electrochemical monitoring of DNA by using differential pulse voltammetry (DPV) in contrast to unmodified one. The effect of both DNA concentration and sonication time was also examined based on the response of q-PGE. The detection limit was calculated to be 8.06 μg/mL at q-PGE. Electrochemical impedance spectroscopy (EIS) was used for the characterization of the surface modification of q-PGE and consequently, the results were found to be in good agreement with the voltammetric measurements.
authors.affiliation: Eskisehir Osmangazi University, Faculty of Arts and Science, Chemistry Department, 26480 Eskisehir, Turkey.
authors.forename: Cansel
authors.initials: C
authors.lastname: Tuncer
authors.forename: Ece
authors.initials: E
authors.lastname: Canavar
authors.forename: Gulsah
authors.initials: G
authors.lastname: Congur
authors.forename: Hakan
authors.initials: H
authors.lastname: Karadeniz
authors.forename: Arzum
authors.initials: A
authors.lastname: Erdem
authors.forename: Vural
authors.initials: V
authors.lastname: Bütün
mesh_terms: D000818:Animals; D060326:Chemistry Techniques, Synthetic; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D006847:Hydrocarbons, Iodinated / Q000737:chemistry; D058105:Polymerization; D011109:Polymethacrylic Acids / Q000138:chemical synthesis* / Q000737:chemistry*; D012995:Solubility; D014867:Water / Q000737:chemistry*
chemical_list: D006847:Hydrocarbons, Iodinated; C457519:PMAA-b-PDMAEMA; D011109:Polymethacrylic Acids; D014867:Water; D004247:DNA; C014055:methyl iodide