title: Genotyping of Staphylococcus aureus isolates from diseased poultry.
abstract: To gain insight into the genomic diversity of Staphylococcus aureus associated with diseases in domestic poultry, 131 isolates from clinically ill turkeys (n=80) and chickens (n = 51) were collected and genotyped using microarray hybridisations. MRSA isolates were subjected to spa and dru typing and their antimicrobial resistance geno- and phenotypes were determined. Most (68 out of 80) turkey isolates belonged to the clonal complex (CC) 398. Seventeen of the 80 isolates (21.2%) were MRSA. The most common MRSA type among turkeys was CC398-MRSA-V (n = 8), but CC5-MRSA-III (n = 4), CC9-MRSA-IV (n = 2), CC398-MRSA-IV (n = 2) and a single CC398-MRSA with an unidentified/truncated SCCmec element were also found. Among the chicken isolates, CC5 predominated (44 out of 51). Five of the chicken isolates were MRSA (9.8%), all belonging to CC398-MRSA-V. These data show that the current dissemination of livestock-associated MRSA also engulfs chickens and turkeys, and that MRSA surveillance among these species is warranted.
authors.affiliation: Institute for Medical Microbiology and Hygiene, Technical University of Dresden, Dresden, Germany; Alere Technologies GmbH, Jena, Germany. Electronic address: monecke@rocketmail.com.
authors.forename: Stefan
authors.initials: S
authors.lastname: Monecke
authors.affiliation: Institute for Medical Microbiology and Hygiene, Technical University of Dresden, Dresden, Germany.
authors.forename: Antje
authors.initials: A
authors.lastname: Ruppelt
authors.affiliation: Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Neustadt-Mariensee, Germany.
authors.forename: Sarah
authors.initials: S
authors.lastname: Wendlandt
authors.affiliation: Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Neustadt-Mariensee, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Schwarz
authors.affiliation: Alere Technologies GmbH, Jena, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Slickers
authors.affiliation: Alere Technologies GmbH, Jena, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Ehricht
authors.affiliation: Poultry Clinics and Laboratory Dr. Pöppel, Delbrück, Germany.
authors.forename: Sonia Cortez de
authors.initials: SC
authors.lastname: Jäckel
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000942:Antigens, Bacterial / Q000235:genetics; D002645:Chickens; D005838:Genotype; D008460:Meat; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D011201:Poultry Diseases / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D014422:Turkeys
chemical_list: D000900:Anti-Bacterial Agents; D000942:Antigens, Bacterial; C119204:streptococcal protective antigen