title: Genotoxic and morphological damage in Hippocampus reidi exposed to crude oil.
abstract: The present article investigates the responses of Hippocampus reidi exposed to crude oil (10mL/L) for time periods that are characteristic of acute (12, 24, 48 and 96h) and subchronic (168 and 336h) and its recovery at 168h, such as to assess H. reidi as a good sentinel species. These responses were examined by genotoxic and morphologic analyses. There was a control group (n=8) for each group exposed to crude oil (n=8). The results revealed the occurrence of genotoxic damage in erythrocytes of all specimens exposed to crude oil. The damage index (DI) observed for all exposure times were significantly higher compared to those of the respective control groups; the highest DI was observed in specimens exposed to crude oil for 168h. The group exposed to crude oil for 96h had the highest values of micronuclei than all other exposure times. Microscopic inspection of the H. reidi gills revealed the presence of several histopathological changes, all of which were minor severity (structural normal gills). The recovery experiment revealed a significant reduction in genotoxic damages, however the period of 168h was not sufficient to recuperate the histopathology damages. The Seahorse has presented significant genotoxic responses after exposure to crude oil. Furthermore, it is a sedentary fish and is widely distributed; in conclusion this specie can be considered an excellent sentinel organism.
authors.affiliation: Applied Ichthyology Laboratory, Vila Velha University-UVV, CEP: 29102-770, Vila Velha, ES, Brazil.
authors.forename: Frederico Augusto
authors.initials: FA
authors.lastname: Cariello Delunardo
authors.forename: Bruno
authors.initials: B
authors.lastname: Ferreira da Silva
authors.forename: Marcelo Gustavo
authors.initials: MG
authors.lastname: Paulino
authors.forename: Marisa
authors.initials: M
authors.lastname: Narciso Fernandes
authors.forename: Adriana Regina
authors.initials: AR
authors.lastname: Chippari-Gomes
mesh_terms: D000818:Animals; D004247:DNA / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects; D005880:Gills / Q000187:drug effects*; D048629:Micronuclei, Chromosome-Defective; D010578:Petroleum / Q000633:toxicity*; D023701:Smegmamorpha / Q000502:physiology*; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D010578:Petroleum; D014874:Water Pollutants, Chemical; D004247:DNA