title: Dietary antioxidant, quercetin, protects sertoli-germ cell coculture from atrazine-induced oxidative damage.
abstract: Quercetin (QT), a dietary-derived flavonoid, is ubiquitous in fruits and vegetables and plays an important role in human health by virtue of its antioxidant function. The present study was designed to examine the effects of QT on oxidative damage that was induced by the herbicide, atrazine (ATZ), in mixed cultures of Sertoli-germ cells. Results showed that treatment with QT increased cell viability and decreased catalase activity, malondialdehyde, and reactive oxygen species (ROS) levels. QT treatment also increased the mRNA expression of glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione-S-transferase, and superoxide dismutase-1 and could not reversed to the control levels ATZ-induced steady-state mRNA levels of these antioxidant genes as well as the level of glutathione and activities of GSH-Px and GR. QT has protective effect against ATZ-induced oxidative stress through a reduction in ROS levels and lipid peroxidation.
authors.affiliation: Department of Chemical Sciences, College of Natural Sciences, Redeemer's University, Redemption City, Ogun State, Nigeria. abarikwus@run.edu.ng
authors.forename: Sunny O
authors.initials: SO
authors.lastname: Abarikwu
authors.forename: Aditya B
authors.initials: AB
authors.lastname: Pant
authors.forename: Ebenezer O
authors.initials: EO
authors.lastname: Farombi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism* / Q000627:therapeutic use; D001280:Atrazine / Q000633:toxicity*; D002450:Cell Communication / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D019587:Dietary Supplements; D004790:Enzyme Induction / Q000187:drug effects; D006540:Herbicides / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D020011:Protective Agents / Q000378:metabolism* / Q000627:therapeutic use; D011794:Quercetin / Q000378:metabolism* / Q000627:therapeutic use; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism*; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D006540:Herbicides; D020011:Protective Agents; D012333:RNA, Messenger; D011794:Quercetin; D010088:Oxidoreductases; D001280:Atrazine