title: Feline polymorphonuclear neutrophils produce pro-inflammatory cytokines following exposure to Microsporum canis.
abstract: The mechanisms involved in the establishment of the specific immune response against dermatophytes remain unknown. Polymorphonuclear neutrophils (PMNs) are recruited early during the infection process and participate in the elimination of dermatophytes. They could therefore be involved in the induction of the immune response during dermatophytoses by producing specific cytokines. The aim of this work was to assess the in vitro cytokine production by feline PMNs exposed to living arthroconidia from the dermatophyte species Microsporum canis or stimulated with either a secreted or a structural component of M. canis, the latter consisting of heat-killed arthroconidia. The levels of specific cytokines produced by PMNs were determined by capture ELISA and/or quantitative RT-PCR. Results showed that PMNs secrete TNFα, IL-1β and IL-8 following exposure to M. canis living arthroconidia and stimulation with both a secreted component and heat-killed arthroconidia. The level of IL-8 mRNA was also increased in PMNs stimulated with M. canis living arthroconidia. In conclusion, infective M. canis arthroconidia induce the production of pro-inflammatory cytokines by feline PMNs that can be activated either by secreted or structural fungal components. Our results suggest that these granulocytes are involved in the initiation of the immune response against M. canis.
authors.affiliation: Veterinary Mycology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
authors.forename: Ludivine
authors.initials: L
authors.lastname: Cambier
authors.affiliation: Veterinary Mycology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
authors.forename: Anne
authors.initials: A
authors.lastname: Mathy
authors.affiliation: Veterinary Mycology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
authors.forename: Aline
authors.initials: A
authors.lastname: Baldo
authors.affiliation: Veterinary Mycology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium; Parasitology and Parasitic Diseases Unit, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Cluj-Napoca, Romania.
authors.forename: Elena Tatiana
authors.initials: ET
authors.lastname: Bagut
authors.affiliation: Laboratory of Venin and Biological Activities, Jean-François Champollion University Center, Albi, France.
authors.forename: Jérémy
authors.initials: J
authors.lastname: Tabart
authors.affiliation: Veterinary Histology, Department of Morphology and Pathology, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
authors.forename: Nadine
authors.initials: N
authors.lastname: Antoine
authors.affiliation: Veterinary Mycology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium. Electronic address: bmignon@ulg.ac.be.
authors.forename: Bernard
authors.initials: B
authors.lastname: Mignon
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000097:blood / Q000276:immunology* / Q000382:microbiology*; D002415:Cats; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis / Q000097:blood / Q000276:immunology*; D003881:Dermatomycoses / Q000097:blood / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D053583:Interleukin-1beta / Q000096:biosynthesis / Q000097:blood / Q000276:immunology; D016209:Interleukin-8 / Q000096:biosynthesis / Q000097:blood / Q000235:genetics / Q000276:immunology; D008297:Male; D008864:Microsporum / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology* / Q000382:microbiology*; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D013172:Spores, Fungal; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000097:blood / Q000276:immunology
chemical_list: D016207:Cytokines; D053583:Interleukin-1beta; D016209:Interleukin-8; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha