title: Regulatory T cell kinetics in the peripheral blood of patients with Crohn's disease.
abstract: Estimates of T regulatory cell populations in the periphery of patients with Crohn's disease are confounded by disease activity and concomitant immunotherapeutic agents known to affect T cell proliferation and survival. We performed deuterium pulse/chase experiments in patients with quiescent Crohn's disease on no immunotherapy and healthy control subjects to estimate T regulatory cell kinetics. Quantification of deuterated DNA isolated from T cell subsets over 10 days was determined by mass spectrophotometry. We demonstrate enhanced proliferation within the T regulatory cell population from patients with Crohn's disease when compared to non-T regulatory cells and T regulatory cells from healthy control subjects. We speculate that T regulatory cells isolated from the periphery of patients with Crohn's disease experience persistent antigen stimulation resulting in excess proliferative rates.
authors.affiliation: Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN 55905, USA..
authors.forename: Meher K
authors.initials: MK
authors.lastname: Rahman
authors.forename: Chetan P
authors.initials: CP
authors.lastname: Offord
authors.forename: Sahil
authors.initials: S
authors.lastname: Khanna
authors.forename: Godfrey C
authors.initials: GC
authors.lastname: Ford
authors.forename: Xuan-Mai T
authors.initials: XM
authors.lastname: Perrson
authors.forename: Phyllis A
authors.initials: PA
authors.lastname: Svingen
authors.forename: Yuning
authors.initials: Y
authors.lastname: Xiong
authors.forename: Zeljko
authors.initials: Z
authors.lastname: Bajzer
authors.forename: William A
authors.initials: WA
authors.lastname: Faubion
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000276:immunology; D001786:Blood Glucose; D016022:Case-Control Studies; D003424:Crohn Disease / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006801:Humans; D007156:Immunologic Memory; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D008875:Middle Aged; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism; D055815:Young Adult
chemical_list: D001786:Blood Glucose