title: Molecular Evaluation of the IFN γ +874, TNF α -308, and IL-1Ra VNTR Sequences in Silicosis.
abstract: BACKGROUND
  to assess whether single nucleotide variation within regulatory sequences of cytokine or chemokine genes is associated with silicosis, this study was conducted for molecular evaluation of the IFN γ +874, TNF α -308, and IL-1Ra VNTR sequences in the patients with the silicosis.
  
  
  METHODS
  ASO-PCR technique was carried out for genotyping of IFN γ +874 and TNF α -308, and in the case of IL-1Ra VNTR, a PCR reaction was performed.
  
  
  RESULTS
  our findings implied that: 1) IFN γ +874 T allele frequency was 0.44 in the cases and 0.48 in the controls; 2) IFN γ +874 A allele frequency was 0.56 in the cases and 0.52 in the controls; 3) TNF α -308 A allele frequency was 0.34 in the cases and 0.29 in the controls; 4) TNF α -308 G allele frequency was 0.66 in the cases and 0.71 in the controls; 5) the observed frequencies (%) of allele 1, allele 2, allele 3 and allele 4 were 65(72.2), 18(20), 2(2.22), 5(5.56) in the cases respectively, and 6) 68(75.6), 17(18.9), 2(2.22), 3(3.33) in the controls respectively. Genotypic and allelic frequencies were not significantly different between cases and controls (p value > 0.05).
  
  
  CONCLUSIONS
  it can be concluded that IFN γ +874, TNF α -308 and IL-1Ra VNTR are not associated with silicosis.
authors.affiliation: Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran.
authors.forename: Isa Abdi
authors.initials: IA
authors.lastname: Rad
authors.forename: Iraj
authors.initials: I
authors.lastname: Mohebbi
authors.forename: Morteza
authors.initials: M
authors.lastname: Bagheri
keywords: IFN γ +874; IL-1Ra VNTR; TNF α -308; silicosis