title: A PCR-RFLP method for typing human papillomavirus type 16 variants.
abstract: Infection with some types of human papillomavirus (HPV) is required for cervical cancer development, being HPV type 16 (HPV 16) the most common type in premalignant and malignant cervical lesions. DNA sequencing has revealed the existence of intratypic variants of HPV 16 whose genotyping is clinically useful for distinguishing between persistent and recurrent infections. From the epidemiological perspective, the frequency of diverse HPV 16 variants in several populations could correlate with the presence of precursor high-risk lesions in different anatomical locations. Currently, the "gold standard" method for identifying HPV 16 variants involves the sequencing of genomic regions to identify characteristic polymorphic sites. Although some other methods have been described, they require specialized or high-cost equipment. In this study, a robust and low cost procedure is described for HPV 16 variant typing, based on the long control region of the virus.
authors.affiliation: Mesoamerican Center of Public Health Studies and Disasters (CEMESAD), Autonomous University of Chiapas (UNACH), Tapachula, Chis, Mexico. angel.lugo@unach.mx
authors.forename: Ángel
authors.initials: Á
authors.lastname: Lugo-Trampe
authors.forename: Karina del C
authors.initials: Kdel C
authors.lastname: Trujillo-Murillo
authors.forename: Iram P
authors.initials: IP
authors.lastname: Rodriguez-Sanchez
authors.forename: Maria del C
authors.initials: Mdel C
authors.lastname: Barboza-Cerda
authors.forename: José de J
authors.initials: Jde J
authors.lastname: Lugo-Trampe
authors.forename: Laura C
authors.initials: LC
authors.lastname: Hernández-Ramirez
authors.forename: Luis M
authors.initials: LM
authors.lastname: Canseco-Avila
authors.forename: Marisol
authors.initials: M
authors.lastname: Espinoza-Ruiz
authors.forename: Sergio
authors.initials: S
authors.lastname: Domínguez-Arrevillaga
authors.forename: Iván
authors.initials: I
authors.lastname: Delgado-Enciso
mesh_terms: D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D005838:Genotype; D052162:Human papillomavirus 16 / Q000145:classification* / Q000235:genetics*; D006801:Humans; D017720:Molecular Epidemiology / Q000191:economics / Q000379:methods; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004279:DNA, Viral