title: Production and characterization of an alloplasmic and monosomic addition line of Brassica rapa carrying the cytoplasm and one chromosome of Moricandia arvensis.
abstract: Intergeneric hybridization was performed between Moricandia arvensis and four inbred lines of Brassica rapa following embryo rescue. Three F(1) hybrid plants were developed from three cross combinations of M. arvensis × B. rapa, and amphidiploids were synthesized by colchicine treatment. Six BC(1) plants were generated from a single cross combination of amphidipolid × B. rapa 'Ko1-303' through embryo rescue. One BC(2) and three BC(3) plants were obtained from successive backcrossing with B. rapa 'Ko1-303' employing embryo rescue. Alloplasmic and monosomic addition lines of B. rapa (Allo-MALs, 2n = 21) were obtained from backcrossed progeny of three BC(3) plants (2n = 21, 22 and 23) without embryo rescue. An alloplasmic line of B. rapa (2n = 20) degenerated before floliation on 1/2 MS medium due to severe chlorosis. Allo-MALs of B. rapa (2n = 21) showed stable male sterility without any abnormal traits in vegetative growth and female fertility. Molecular analyses revealed that the same chromosome and cytoplasm of M. arvensis had been added to each Allo-MAL of B. rapa. This Allo-MAL of B. rapa may be useful material for producing cytoplasmic male sterile lines of B. rapa.
authors.affiliation: Laboratory of Plant Breeding, Faculty of Agriculture, Utsunomiya University , 350 Minemachi, Utsunomiya, Tochigi 321-8505, Japan ; United Graduate School of Agriculture, Tokyo University of Agriculture and Technology , 3-5-8 Saiwai, Fuchu, Tokyo 183-8509, Japan.
authors.forename: Kota
authors.initials: K
authors.lastname: Tsutsui
authors.forename: Bum Hee
authors.initials: BH
authors.lastname: Jeong
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Ito
authors.forename: Sang Woo
authors.initials: SW
authors.lastname: Bang
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kaneko
keywords: Brassica rapa; Moricandia arvensis; alloplasmic line; chlorosis; cytoplasmic male sterility; intergeneric hybridization; monosomic addition line