title: Ethanol production from Saccharina japonica using an optimized extremely low acid pretreatment followed by simultaneous saccharification and fermentation.
abstract: An extremely low acid (ELA) pretreatment using 0.06% (w/w) sulfuric acid at 170 °C for 15 min was employed to extract non-glucan components from Saccharina japonica, a brown macroalgae. Subsequent simultaneous saccharification and fermentation (SSF) was conducted using Saccharomyces cerevisiae DK 410362 and cellulase (15 FPU/g-glucan) and ß-glucosidase (70 pNPGU/g-glucan). Deionized water was used for making fermentation suspension. After the ELA pretreatment, a glucan content of 29.10% and an enzymatic digestibility of 83.96% was obtained for pretreated S. japonica. These values are 4.2- and 2.4-fold higher, respectively, than those of obtained with untreated S. japonica. In SSF, a bioethanol concentration of 6.65 g/L was obtained, corresponding to a glucose equivalent concentration of 13.01 g/L, which indicated an SSF yield of 67.41% based on the total available glucan of the pretreated S. japonica. The remaining separated liquid hydrolysate, which contains mannitol and alginate-derived oligosaccharides can be applied to other fermentations.
authors.affiliation: Department of Applied Chemical Engineering, Dankook University, Cheonan, Chungnam 330-714, South Korea.
authors.forename: Ji ye
authors.initials: Jy
authors.lastname: Lee
authors.forename: Pan
authors.initials: P
authors.lastname: Li
authors.forename: Jieun
authors.initials: J
authors.lastname: Lee
authors.forename: Hyun Jin
authors.initials: HJ
authors.lastname: Ryu
authors.forename: Kyeong Keun
authors.initials: KK
authors.lastname: Oh
mesh_terms: D056804:Biofuels; D002480:Cellulase / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005936:Glucans / Q000032:analysis; D006868:Hydrolysis; D000459:Phaeophyceae / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013464:Sulfuric Acids / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D056804:Biofuels; D005936:Glucans; D013464:Sulfuric Acids; D000431:Ethanol; D001617:beta-Glucosidase; D002480:Cellulase; C033158:sulfuric acid