title: Expression of prototype foamy virus pol as a Gag-Pol fusion protein does not change the timing of reverse transcription.
abstract: Foamy viruses are retroviruses whose Pol protein is synthesized without Gag from a spliced mRNA. Unlike orthoretroviruses, reverse transcription occurs during viral assembly, leading to DNA-containing virions. When prototype foamy virus Pol is expressed as an orthoretroviral-like Gag-Pol fusion protein, reverse transcription also occurs late in viral replication, as measured by the timing of reverse transcriptase sensitivity to the inhibitor 3'-azido-3'deoxythymidine (AZT). Thus, timing of reverse transcription is intrinsic to Pol itself.
authors.affiliation: Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
authors.forename: Dana L
authors.initials: DL
authors.lastname: Jackson
authors.forename: Eun-Gyung
authors.initials: EG
authors.lastname: Lee
authors.forename: Maxine L
authors.initials: ML
authors.lastname: Linial
mesh_terms: D002460:Cell Line; D015685:Fusion Proteins, gag-pol / Q000096:biosynthesis* / Q000235:genetics*; D015967:Gene Expression Regulation, Viral; D006801:Humans; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D018894:Reverse Transcriptase Inhibitors / Q000378:metabolism; D048348:Reverse Transcription; D016092:Spumavirus / Q000235:genetics* / Q000502:physiology; D019065:Virus Assembly; D015215:Zidovudine / Q000378:metabolism
chemical_list: D015685:Fusion Proteins, gag-pol; D018894:Reverse Transcriptase Inhibitors; D015215:Zidovudine; D012194:RNA-Directed DNA Polymerase