title: Development of self-compatible B. rapa by RNAi-mediated S locus gene silencing.
abstract: The self-incompatibility (SI) system is genetically controlled by a single polymorphic locus known as the S-locus in the Brassicaceae. Pollen rejection occurs when the stigma and pollen share the same S-haplotype. Recognition of S-haplotype specificity has recently been shown to involve at least two S-locus genes, S-receptor kinase (SRK) and S-locus protein 11 or S locus Cysteine-rich (SP11/SCR) protein. Here, we examined the function of S(60), one SP11/SCR allele of B. rapa cv. Osome, using a RNAi-mediated gene silencing approach. The transgenic RNAi lines were highly self-compatible, and this trait was stable in subsequent generations, even after crossing with other commercial lines. These findings also suggested that the resultant self-compatibility could be transferred to commercial cultivars with the desired performances in B. rapa.
authors.affiliation: Department of Horticulture, Sunchon National University, Suncheon, Republic of Korea.
authors.forename: Hee-Jeong
authors.initials: HJ
authors.lastname: Jung
authors.forename: Hyo-Jin
authors.initials: HJ
authors.lastname: Jung
authors.forename: Nasar Uddin
authors.initials: NU
authors.lastname: Ahmed
authors.forename: Jong-In
authors.initials: JI
authors.lastname: Park
authors.forename: Kwon-Kyoo
authors.initials: KK
authors.lastname: Kang
authors.forename: Yoonkang
authors.initials: Y
authors.lastname: Hur
authors.forename: Yong-Pyo
authors.initials: YP
authors.lastname: Lim
authors.forename: Ill-Sup
authors.initials: IS
authors.lastname: Nou
mesh_terms: D029741:Brassica rapa / Q000235:genetics / Q000502:physiology*; D003433:Crosses, Genetic; D005306:Fertilization; D006023:Glycoproteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D006239:Haplotypes; D010940:Plant Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D030821:Plants, Genetically Modified / Q000502:physiology; D011058:Pollen / Q000235:genetics; D034622:RNA Interference; D012098:Reproduction / Q000235:genetics
chemical_list: D006023:Glycoproteins; D010940:Plant Proteins; C064156:self-incompatibility locus specific glycoprotein, Brassica