title: Analysis of insect-induced volatiles from rice.
abstract: Plants emit volatile organic compounds (VOCs) from various organs. To understand the biological and ecological roles of plant VOCs as well as their biosynthesis, it is important to determine the quality and quantity of individual plant VOCs. Here we describe three techniques, including solid-phase microextraction, closed-loop stripping system, and an open dynamic system, for sampling volatiles emitted from insect-damaged rice plants. In addition, the protocol for using gas chromatography-mass spectrometry to elucidate the chemical identities of rice volatiles is described.
authors.affiliation: Department of Plant Sciences, University of Tennessee, Knoxville, TN, USA.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhao
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zhuang
authors.forename: Gitika
authors.initials: G
authors.lastname: Shrivastava
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
mesh_terms: D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007313:Insecta; D012275:Oryza / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods; D055549:Volatile Organic Compounds / Q000737:chemistry*
chemical_list: D055549:Volatile Organic Compounds