title: ChIP-Chip Identifies SEC23A, CFDP1, and NSD1 as TFII-I Target Genes in Human Neural Crest Progenitor Cells.
abstract: Objectives :  GTF2I and GTF2IRD1 genes located in Williams-Beuren syndrome (WBS) critical region encode TFII-I family transcription factors. The aim of this study was to map genomic sites bound by these proteins across promoter regions of developmental regulators associated with craniofacial development. Design :  Chromatin was isolated from human neural crest progenitor cells and the DNA-binding profile was generated using the human RefSeq tiling promoter ChIP-chip arrays. Results :  TFII-I transcription factors are recruited to the promoters of SEC23A, CFDP1, and NSD1 previously defined as TFII-I target genes. Moreover, our analysis revealed additional binding elements that contain E-boxes and initiator-like motifs. Conclusions :  Genome-wide promoter binding studies revealed SEC23A, CFDP1, and NSD1 linked to craniofacial or dental development as direct TFII-I targets. Developmental regulation of these genes by TFII-I factors could contribute to the WBS-specific facial dysmorphism.
authors.forename: Aleksandr V
authors.initials: AV
authors.lastname: Makeyev
authors.forename: Dashzeveg
authors.initials: D
authors.lastname: Bayarsaihan
mesh_terms: D006801:Humans; D009432:Neural Crest; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics; D013234:Stem Cells; D019473:Transcription Factors, TFII / Q000235:genetics; D018980:Williams Syndrome / Q000235:genetics
chemical_list: D011506:Proteins; D019473:Transcription Factors, TFII