title: Heme oxygenase-1-mediated reactive oxygen species reduction is involved in the inhibitory effect of curcumin on lipopolysaccharide-induced monocyte chemoattractant protein-1 production in RAW264.7 macrophages.
abstract: Monocyte chemoattractant protein 1 (MCP-1) is a CC chemokine which stimulates mononuclear leukocytes and is significant in the pathogenesis of inflammatory disease. Curcumin, extracted from Curcumae longae, has been shown to possess anti-inflammatory activity, in inflammation associated with the induction of MCP-1 expression. However, the underlying mechanisms involved in the effect of curcumin on MCP-1 expression remain unclear. In the current study, we investigated the effect of curcumin on the production of MCP-1 induced by lipopolysaccharide (LPS) in macrophages and the possible mechanisms involved. The results revealed that curcumin decreased MCP-1 production in a concentration-dependent manner and reduced the generation of reactive oxygen species (ROS) induced by LPS in RAW264.7 macrophages. Additionally, zinc protoporphyrin, a heme oxygenase-1 (HO-1) inhibitor, blocked the inhibitory effect of curcumin on the LPS-induced MCP-1 expression. The exposure of cells to curcumin was found to enhance HO-1 expression. Furthermore, additional experiments indicated that the inhibitory effect of curcumin on LPS-induced MCP-1 expression was significantly attenuated in the presence of N-acetylcysteine (an effective ROS scavenger). The results presented in our study suggest that curcumin enhances the expression of HO-1 to reduce the LPS-induced production of ROS, which leads to the inhibition of MCP-1 expression in RAW264.7 macrophages.
authors.affiliation: Department of Cardiology, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan 646000, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhong
authors.forename: Tingrong
authors.initials: T
authors.lastname: Liu
authors.forename: Wenyan
authors.initials: W
authors.lastname: Lai
authors.forename: Ying
authors.initials: Y
authors.lastname: Tan
authors.forename: Di
authors.initials: D
authors.lastname: Tian
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Guo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000633:toxicity; D002460:Cell Line; D018932:Chemokine CCL2 / Q000096:biosynthesis*; D003474:Curcumin / Q000494:pharmacology* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D051547:Heme Oxygenase-1 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D011524:Protoporphyrins / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D018932:Chemokine CCL2; D008070:Lipopolysaccharides; D011524:Protoporphyrins; D017382:Reactive Oxygen Species; C017803:zinc protoporphyrin; D051547:Heme Oxygenase-1; D003474:Curcumin