title: A simple high-performance liquid chromatography method for simultaneous determination of three triazole antifungals in human plasma.
abstract: A rapid and simple high-performance liquid chromatography (HPLC) assay was developed for the simultaneous determination of three triazole antifungals (voriconazole, posaconazole, and itraconazole and the metabolite of itraconazole, hydroxyitraconazole) in human plasma. Sample preparation involved a simple one-step protein precipitation with 1.0 M perchloric acid and methanol. After centrifugation, the supernatant was injected directly into the HPLC system. Voriconazole, posaconazole, itraconazole, its metabolite hydroxyitraconazole, and the internal standard naproxen were resolved on a C(6)-phenyl column using gradient elution of 0.01 M phosphate buffer, pH 3.5, and acetonitrile and detected with UV detection at 262 nm. Standard curves were linear over the concentration range of 0.05 to 10 mg/liter (r(2) > 0.99). Bias was <8.0% from 0.05 to 10 mg/liter, intra- and interday coefficients of variation (imprecision) were <10%, and the limit of quantification was 0.05 mg/liter.
authors.affiliation: Clinical Pharmacology, Department of Medicine, University of Otago-Christchurch, Christchurch, New Zealand. mei.zhang@cdhb.govt.nz
authors.forename: Mei
authors.initials: M
authors.lastname: Zhang
authors.forename: Grant A
authors.initials: GA
authors.lastname: Moore
authors.forename: Murray L
authors.initials: ML
authors.lastname: Barclay
authors.forename: Evan J
authors.initials: EJ
authors.lastname: Begg
mesh_terms: D000935:Antifungal Agents / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D017964:Itraconazole / Q000031:analogs & derivatives* / Q000097:blood*; D057230:Limit of Detection; D009288:Naproxen / Q000097:blood; D015588:Observer Variation; D011743:Pyrimidines / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D014230:Triazoles / Q000097:blood*; D065819:Voriconazole
chemical_list: D000935:Antifungal Agents; D011743:Pyrimidines; D014230:Triazoles; C084794:hydroxyitraconazole; D017964:Itraconazole; D009288:Naproxen; C101425:posaconazole; D065819:Voriconazole