title: Three-dimensional localization of the α and β subunits and of the II-III loop in the skeletal muscle L-type Ca2+ channel.
abstract: The L-type Ca(2+) channel (dihydropyridine receptor (DHPR) in skeletal muscle acts as the voltage sensor for excitation-contraction coupling. To better resolve the spatial organization of the DHPR subunits (α(1s) or Ca(V)1.1, α(2), β(1a), δ1, and γ), we created transgenic mice expressing a recombinant β(1a) subunit with YFP and a biotin acceptor domain attached to its N- and C- termini, respectively. DHPR complexes were purified from skeletal muscle, negatively stained, imaged by electron microscopy, and subjected to single-particle image analysis. The resulting 19.1-Å resolution, three-dimensional reconstruction shows a main body of 17 × 11 × 8 nm with five corners along its perimeter. Two protrusions emerge from either face of the main body: the larger one attributed to the α(2)-δ1 subunit that forms a flexible hook-shaped feature and a smaller protrusion on the opposite side that corresponds to the II-III loop of Ca(V)1.1 as revealed by antibody labeling. Novel features discernible in the electron density accommodate the atomic coordinates of a voltage-gated sodium channel and of the β subunit in a single docking possibility that defines the α1-β interaction. The β subunit appears more closely associated to the membrane than expected, which may better account for both its role in localizing the α(1s) subunit to the membrane and its suggested role in excitation-contraction coupling.
authors.affiliation: Department of Anesthesia, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: John
authors.initials: J
authors.lastname: Szpyt
authors.forename: Nancy
authors.initials: N
authors.lastname: Lorenzon
authors.forename: Claudio F
authors.initials: CF
authors.lastname: Perez
authors.forename: Ethan
authors.initials: E
authors.lastname: Norris
authors.forename: Paul D
authors.initials: PD
authors.lastname: Allen
authors.forename: Kurt G
authors.initials: KG
authors.lastname: Beam
authors.forename: Montserrat
authors.initials: M
authors.lastname: Samsó
mesh_terms: D000818:Animals; D020746:Calcium Channels, L-Type / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure*; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D008854:Microscopy, Electron; D062105:Molecular Docking Simulation; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure*; D018482:Muscle, Skeletal / Q000378:metabolism / Q000648:ultrastructure*; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D021122:Protein Subunits
chemical_list: D020746:Calcium Channels, L-Type; D009124:Muscle Proteins; D021122:Protein Subunits