title: [Effect of calcium ionophore A23187 plus IFN-γ on dendritic cells derived from peripheral blood mononuclear cells].
abstract: OBJECTIVE
  To explore the effect of calcium ionophore (CI) A23187 plus IFN-γ on dendritic cells (DC) from healthy human peripheral blood mononuclear cells (PBMNC).
  
  
  METHODS
  PBMNC from healthy donors were treated with GM-CSF plus IL-4, A23187, and A23187 plus IFN-γ, respectively. After culture for 72 h, the change of cellular morphology was observed under light microscope and electron microscope. Surface markers on DC were analyzed by flow cytometry. MTT colorimetry was used to detect the proliferation of allogeneic T cells. Plasma concentrations of IL-12 and IFN-γ were measured by ELISA.
  
  
  RESULTS
  PBMNC treated with A23187 plus IFN-γ for 72 h presented DC with typical morphology effectively. The surface markers CD40, CD83, and CD86 were obviously increased in group A23187 plus IFN-γ (P<0.01), but decreased in CD1a (P<0.01). In addition, it evidently stimulated the proliferation of allogeneic T cells. The levels of IL-12 and IFN-γ were significantly increased compared with other groups (P<0.01).
  
  
  CONCLUSIONS
  A23187 plus IFN-γ can effectively enhance marked transformation of PBMNC into DC.
authors.affiliation: Department of Oncology, Central South University, Haikou, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Meng
authors.forename: Dawei
authors.initials: D
authors.lastname: Peng
authors.forename: Xuelan
authors.initials: X
authors.lastname: Zuo
mesh_terms: D000001:Calcimycin / Q000494:pharmacology*; D061207:Calcium Ionophores / Q000494:pharmacology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism / Q000494:pharmacology*; D018664:Interleukin-12 / Q000378:metabolism; D015847:Interleukin-4 / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology
chemical_list: D061207:Calcium Ionophores; D018664:Interleukin-12; D015847:Interleukin-4; D000001:Calcimycin; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor