title: Identification, characterisation, and quantification of phenolic compounds in the antioxidant activity-containing fraction from the seeds of Korean perilla (Perilla frutescens) cultivars.
abstract: The present research was the first to investigate phenolic compound profiles and antioxidant properties in the seeds of various perilla (Perilla frutescens) cultivars. The 80% methanol extract (50 μg/ml) of this species showed potent antioxidant activities against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radicals. Phenolic compounds were characterised by nuclear magnetic resonance (NMR) spectroscopy, and ultra performance liquid chromatography with photodiode array detector and electrospray ionisation/mass (UPLC-PDA-ESI/MS) analysis. Nine compounds were elucidated as caffeic acid-3-O-glucoside (1), caffeic acid (2), luteolin-7-O-glucoside (3), apigenin-7-O-glucoside (4), rosmarinic acid-3-O-glucoside (5), rosmarinic acid (6), luteolin (7), apigenin (8), and chrysoeriol (9). The individual and total phenolic contents were remarkably different, especially rosmarinic acid-3-O-glucoside (5) and rosmarinic acid (6) which were the predominant compounds (>95%) in all perilla cultivars. Additionally, Yeupsil cultivar exhibited the highest phenolic content (5029.0 μg/g) and antioxidant activity, whereas the lowest was shown by Dasil (2138.7 μg/g). Therefore, these results suggest that antioxidant effects of perilla seeds are correlated with phenolic contents.
authors.affiliation: Department of Monitoring and Analysis, NAKDONG River Basin Environmental Office, Ministry of Environment, Changwon 641-722, Republic of Korea.
authors.forename: Jin Hwan
authors.initials: JH
authors.lastname: Lee
authors.forename: Ki Hun
authors.initials: KH
authors.lastname: Park
authors.forename: Myoung-Hee
authors.initials: MH
authors.lastname: Lee
authors.forename: Hyun-Tae
authors.initials: HT
authors.lastname: Kim
authors.forename: Woo Duck
authors.initials: WD
authors.lastname: Seo
authors.forename: Jun Young
authors.initials: JY
authors.lastname: Kim
authors.forename: In-Youl
authors.initials: IY
authors.lastname: Baek
authors.forename: Dae Sik
authors.initials: DS
authors.lastname: Jang
authors.forename: Tae Joung
authors.initials: TJ
authors.lastname: Ha
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D031344:Perilla frutescens / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D056910:Republic of Korea; D012639:Seeds / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts