title: Cytotoxic cholestane-type and ergostane-type steroids from the aerial parts of Euphorbia altotibetic.
abstract: Phytochemical investigation of the ethanol extract obtained from the aerial parts of the Euphorbia altotibetic PAULS. Grown in China resulted in the isolation of three new cholestane-type and three new ergostane-type steroids (cholest-5-en-2β, 4β-diol; cholest-5-en-1β, 4β-diol; cholest-5-en-1α, 3β, 4α -triol; (22E)-ergosta-7,9,22-trien- 3β-ol β-D-glucoside; 5α-methoxy-(22E)-ergosta-7,9,22-trien-3β-ol β-D-glucoside; 6β- methoxy-(22E)-ergosta-7,9,22-trien-3β-ol β-D-glucoside), along with seven known compounds. Their structures were established by extensive one- and two-dimensional NMR spectroscopy, as well as other spectrum and chemical analysis. The isolated new steroids exhibited potent anti-tumor activity against the HeLa cell and Hep-G2 cell with the 50% inhibiting concentration values ranging from 1.9 to 9.2 μg/mL.
authors.affiliation: Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, PR China.
authors.forename: Bei-Bei
authors.initials: BB
authors.lastname: Zhang
authors.forename: Xue-Lian
authors.initials: XL
authors.lastname: Han
authors.forename: Qian
authors.initials: Q
authors.lastname: Jiang
authors.forename: Zhi-Xin
authors.initials: ZX
authors.lastname: Liao
authors.forename: Heng-Shan
authors.initials: HS
authors.lastname: Wang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002776:Cholestanes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D028481:Euphorbia / Q000737:chemistry*; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D035261:Plant Components, Aerial / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002776:Cholestanes; D010936:Plant Extracts; D004875:Ergosterol