title: Induction of rat facial nerve regeneration by functional collagen scaffolds.
abstract: Nerve conduit provides a promising strategy for nerve regeneration, and the proper microenvironment in the lumen could improve the regeneration. Our previous work had demonstrated that linear ordered collagen scaffold (LOCS) could effectively guide the oriented growth of axons. Laminin is known as an important nerve growth promoting factor and can facilitate the growth cone formation. In addition, ciliary neurotrophic factor (CNTF) and brain-derived neurotrophic factor (BDNF) can effectively improve the nerve regeneration after nerve injuries. However, in practice, diffusion caused by the body fluids is the major obstacle in their applications. To retain CNTF or BDNF on the scaffolds, we produced collagen binding CNTF (CBD-CNTF), collagen binding BDNF (CBD-BDNF) and laminin binding CNTF (LBD-CNTF), laminin binding BDNF (LBD-BDNF) respectively. In this work, we developed laminin modified LOCS fibers (L × LOCS) by chemical cross-linking LOCS fibers with laminin. Collagen binding or laminin binding neurotrophic factors were combined with LOCS or L × LOCS, and then filled them into the collagen nerve conduit. They were found to guide the ordered growth of axons, and improve the nerve functional recovery in the rat facial nerve transection model. The combination of CNTF and BDNF greatly enhanced the facial nerve regeneration and functional recovery.
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 3 Nanyitiao, Zhongguancun, Beijing 100190, China.
authors.forename: Jiani
authors.initials: J
authors.lastname: Cao
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Xiao
authors.forename: Wei
authors.initials: W
authors.lastname: Jin
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.forename: Danqing
authors.initials: D
authors.lastname: Meng
authors.forename: Wenyong
authors.initials: W
authors.lastname: Ding
authors.forename: Sufang
authors.initials: S
authors.lastname: Han
authors.forename: Xiaoshan
authors.initials: X
authors.lastname: Hou
authors.forename: Tiansheng
authors.initials: T
authors.lastname: Zhu
authors.forename: Baoyu
authors.initials: B
authors.lastname: Yuan
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Weibang
authors.initials: W
authors.lastname: Liang
authors.forename: Jianwu
authors.initials: J
authors.lastname: Dai
mesh_terms: D000818:Animals; D003094:Collagen / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D020220:Facial Nerve Injuries / Q000473:pathology / Q000503:physiopathology* / Q000628:therapy*; D005260:Female; D048091:Guided Tissue Regeneration / Q000295:instrumentation; D053769:Nanocapsules / Q000008:administration & dosage*; D009414:Nerve Growth Factors / Q000008:administration & dosage*; D009416:Nerve Regeneration / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D054457:Tissue Scaffolds; D016896:Treatment Outcome
chemical_list: D053769:Nanocapsules; D009414:Nerve Growth Factors; D003094:Collagen