title: Image processing of 2D crystal images.
abstract: Electron crystallography of membrane proteins uses cryo-transmission electron microscopy to image frozen-hydrated 2D crystals. The processing of recorded images exploits the periodic arrangement of the structures in the images to extract the amplitudes and phases of diffraction spots in Fourier space. However, image imperfections require a crystal unbending procedure to be applied to the image before evaluation in Fourier space. We here describe the process of 2D crystal image unbending, using the 2dx software system.
authors.affiliation: C-CINA, Biozentrum, University Basel, Basel, Switzerland.
authors.forename: Marcel
authors.initials: M
authors.lastname: Arheit
authors.forename: Daniel
authors.initials: D
authors.lastname: Castaño-Díez
authors.forename: Raphaël
authors.initials: R
authors.lastname: Thierry
authors.forename: Bryant R
authors.initials: BR
authors.lastname: Gipson
authors.forename: Xiangyan
authors.initials: X
authors.lastname: Zeng
authors.forename: Henning
authors.initials: H
authors.lastname: Stahlberg
mesh_terms: D000465:Algorithms; D003461:Crystallography / Q000379:methods*; D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry; D012984:Software
chemical_list: D008565:Membrane Proteins