title: MicroRNAs as markers for neurally committed CD133+/CD34+ stem cells derived from human umbilical cord blood.
abstract: Neural differentiation of the CD133+/CD34+ subpopulation of human umbilical cord blood stem cells was investigated, and neuro-miR (mir-9 and mir-124) expression was examined. An efficient induction protocol for neural differentiation of hematopoietic stem cells together with the exclusion of retinoic acid in this process was also studied. Transcription of some neural markers such as microtubule-associated protein-2, beta-tubulin III, and neuron-specific enolase was evaluated by real-time PCR, immunocytochemistry, and western blotting. Increased expression of neural indicators in the treated cells confirmed the appropriate neural differentiation, which supported the high efficiency of our defined neuronal induction protocol. Verified high expression of neuro-miRNAs along with neuronal specific proteins not only strengthens the regulatory role of miRNAs in determining stem cell fate but also introduces these miRNAs as novel indicators of neural differentiation. These data highlight the prominent therapeutic potential of hematopoietic stem cells for use in cell therapy of neurodegenerative diseases.
authors.affiliation: Stem Cell Biology Department, Stem Cell Technology Research Center, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Hafizi
authors.forename: Amir
authors.initials: A
authors.lastname: Atashi
authors.forename: Behnaz
authors.initials: B
authors.lastname: Bakhshandeh
authors.forename: Mahboubeh
authors.initials: M
authors.lastname: Kabiri
authors.forename: Samad
authors.initials: S
authors.lastname: Nadri
authors.forename: Reza Haji
authors.initials: RH
authors.lastname: Hosseini
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000096:biosynthesis* / Q000097:blood / Q000302:isolation & purification; D018952:Antigens, CD34 / Q000096:biosynthesis* / Q000097:blood / Q000302:isolation & purification; D002454:Cell Differentiation / Q000235:genetics*; D002478:Cells, Cultured; D005260:Female; D005312:Fetal Blood / Q000166:cytology / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis* / Q000097:blood / Q000302:isolation & purification; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D010455:Peptides / Q000097:blood / Q000302:isolation & purification
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D006023:Glycoproteins; C509207:MIRN124 microRNA, human; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; C000606023:PROM1 protein, human; D010455:Peptides