title: Expression of nogo-a is decreased with increasing gestational age in the human fetal brain.
abstract: Nogo is a member of the reticulon family. Our understanding of the physiological functions of the Nogo-A protein has grown over the last few years, and this molecule is now recognized as one of the most important axonal regrowth inhibitors present in central nervous system (CNS) myelin. Nogo-A plays other important roles in nervous system development, epilepsy, vascular physiology, muscle pathology, stroke, inflammation, and CNS tumors. Since the exact role of Nogo-A protein in human brain development is still poorly understood, we studied its cellular and regional distribution by immunohistochemistry in the frontal lobe of 30 human fetal brains. Nogo-A was expressed in the following cortical zones: ependyma, ventricular zone, subventricular zone, intermediate zone, subplate, cortical plate, and marginal zone. The number of positive cells decreased significantly with increasing gestational age in the subplate and marginal zone. Using different antibodies, changes in isoform expression and dimerization states could be shown between various cortical zones. The results demonstrate a significant change in the expression of Nogo-A during the development of the human brain. The effects of its time- and region-specific regulation have to be further studied in detail.
authors.affiliation: Department of Neuropathology, Institute of Pathology, Medical University Graz, Graz, Austria.
authors.forename: J
authors.initials: J
authors.lastname: Haybaeck
authors.forename: I C
authors.initials: IC
authors.lastname: Llenos
authors.forename: R J
authors.initials: RJ
authors.lastname: Dulay
authors.forename: K
authors.initials: K
authors.lastname: Bettermann
authors.forename: C L
authors.initials: CL
authors.lastname: Miller
authors.forename: T
authors.initials: T
authors.lastname: Wälchli
authors.forename: K
authors.initials: K
authors.lastname: Frei
authors.forename: D
authors.initials: D
authors.lastname: Virgintino
authors.forename: M
authors.initials: M
authors.lastname: Rizzi
authors.forename: S
authors.initials: S
authors.lastname: Weis
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000918:Antibody Specificity; D015153:Blotting, Western; D001921:Brain / Q000196:embryology; D001923:Brain Chemistry / Q000502:physiology*; D000939:Epitopes; D005260:Female; D005333:Fetus / Q000378:metabolism*; D005865:Gestational Age; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008969:Molecular Sequence Data; D009185:Myelin Proteins / Q000096:biosynthesis* / Q000235:genetics; D000070798:Nogo Proteins; D011247:Pregnancy; D015203:Reproducibility of Results
chemical_list: D000939:Epitopes; D009185:Myelin Proteins; D000070798:Nogo Proteins; C000603199:RTN4 protein, human