title: ¹H, ¹³C and ¹⁵N resonance assignments of the N-terminal domain of Vta1-Vps60 peptide complex.
abstract: Vta1 and Vps60 are two ESCRT associated proteins, their direct interaction enhances Vps4 ATPase activity. The N-terminal domain of Vta1 (residues 1-167aa, named as Vta1NTD) contains two tandem MIT domains, which specifically recognize Vps60 and Did2 but not other ESCRT-III subunits. The fragment Vps60 (128-186aa) was reported to display full activity of Vps60, which stimulates Vps4 ATPase in a Vta1-dependent manner. To study the structural basis for the interaction between Vta1 and Vps60, as a first step, here, we report the resonance assignments of the sequential backbone atoms and the side chains of the residues in the two components of Vta1NTD/Vps60(128-186) complex at pH 7.0 and 20 °C (BMRB No. 18521).
authors.affiliation: State Key Laboratory of Natural Products and Bioorganic Chemistry, Shanghai Institute of Organic Chemistry, CAS, 345 Lingling Rd., Shanghai 200032, China.
authors.forename: Zhongzheng
authors.initials: Z
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Shen
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.forename: Cody
authors.initials: C
authors.lastname: Vild
authors.forename: Wenxian
authors.initials: W
authors.lastname: Lan
authors.forename: Maili
authors.initials: M
authors.lastname: Liu
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Xu
authors.forename: Chunyang
authors.initials: C
authors.lastname: Cao
mesh_terms: D000595:Amino Acid Sequence; D002247:Carbon Isotopes; D056827:Endosomal Sorting Complexes Required for Transport / Q000737:chemistry* / Q000378:metabolism*; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011522:Protons
chemical_list: D002247:Carbon Isotopes; D056827:Endosomal Sorting Complexes Required for Transport; D009587:Nitrogen Isotopes; D010455:Peptides; D011522:Protons