title: Quinalphos induced oxidative stress and histoarcheitectural alterations in adult male albino rats.
abstract: Quinalphos is a synthetic organophosphate used as a broad spectrum insecticide and acaricide. The present study investigates the effect of three sub-lethal doses (0.52, 1.04, 2.6 mg/kg b.wt) of quinalphos for variable durations (15, 30 and 90 days) on oxidative stress and histopathological changes in adult male rats. Quinalphos treatment for 15 and 30 days resulted in a dose dependent significant increase in malondialdehyde (MDA) levels and glutathione-S-transferase (GST) activity together with a concurrent decrease in ferric reducing ability of plasma (FRAP) and glutathione (GSH) content. Quinalphos treatment for 90 days also induced a significant increase in MDA levels and GST activity but the effect was not dose-dependent. Histopathological examination of liver revealed architectural disarray and dilatation of sinusoids, focal fatty changes, accumulation of eosinophils and single cell necrosis with increasing doses. However, spleen and kidney did not show any histological changes. Administration of quinalphos resulted in oxidative stress and free radical induced injury as evidenced by increased lipid peroxidation, decreased FRAP and histopathological changes in liver.
authors.affiliation: Department of Biochemistry, University College of Medical Sciences and GTB Hospital, University of Delhi, Dilshad Garden, Delhi 110095, India.
authors.forename: Mythily
authors.initials: M
authors.lastname: Subramaneyaan
authors.forename: Smita
authors.initials: S
authors.lastname: Jain
authors.forename: Chandrashekhar
authors.initials: C
authors.lastname: Yadav
authors.forename: Vinod K
authors.initials: VK
authors.lastname: Arora
authors.forename: Basu D
authors.initials: BD
authors.lastname: Banerjee
authors.forename: Rafat S
authors.initials: RS
authors.lastname: Ahmed
mesh_terms: D000818:Animals; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007306:Insecticides / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009946:Organothiophosphorus Compounds / Q000633:toxicity*; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007306:Insecticides; D009946:Organothiophosphorus Compounds; C009145:quinalphos; D008315:Malondialdehyde; D005982:Glutathione Transferase; D005978:Glutathione