title: Effect of bone marrow mesenchymal stem cell transplant on synovial proliferation in rats with type II collagen-induced arthritis.
abstract: OBJECTIVE
  To investigate the influence and mechanism of bone marrow mesenchymal stem cell transplant in the synovial proliferation of type II collagen-induced arthritis.
  
  
  METHODS
  From the bone marrow of Sprague-Dawley rats, mesenchymal stem cells were isolated and expanded. Forty rats were randomly divided into 5 groups: normal control, early mesenchymal stem cell treatment, late mesenchymal stem cell treatment, early collagen-induced arthritis control, and late collagen-induced arthritis control. The mesenchymal stem cells and normal saline were injected through the tail vein, and the following parameters were observed: arthritis index, articular pathology changes, serum vascular endothelial growth factor level, tumor necrosis factor-?, and interluekin-17 levels as detected through stable enzyme-linked immunosorbent assay.
  
  
  RESULTS
  The arthritis index and articular pathologic scores of the early and late treatment groups were lower compared with those of the control groups (P < .05). The arthritis index and articular pathologic scores of the late treatment group were lower than those of the early treatment group (P < .05). The levels of vascular endothelial growth factor, tumor necrosis factor-α, and interluekin-17 of the early and late treatment groups were significantly decreased compared with the collagen-induced arthritis control groups (P < .05), and these levels were positively correlated with the arthritis index and articular pathologic scores (P < .05).
  
  
  CONCLUSIONS
  The transplant of mesenchymal stem cells in rats with collagen-induced arthritis can inhibit the proliferation of synovium, which may be attributed to the reduced expression of vascular endothelial growth factor, tumor necrosis factor-α, and interluekin-17.
authors.affiliation: From the Department of Rheumatology and Immunology, No. 3 People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China. ftjwcn@163.com
authors.forename: Fu-Tao
authors.initials: FT
authors.lastname: Zhao
authors.forename: Ji-Wei
authors.initials: JW
authors.lastname: Yin
authors.forename: Qing-Fen
authors.initials: QF
authors.lastname: Liu
authors.forename: Su-Fen
authors.initials: SF
authors.lastname: Xu
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000097:blood / Q000139:chemically induced / Q000473:pathology / Q000601:surgery*; D015415:Biomarkers / Q000097:blood; D049109:Cell Proliferation; D002478:Cells, Cultured; D024043:Collagen Type II; D015536:Down-Regulation; D020381:Interleukin-17 / Q000097:blood; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013583:Synovial Membrane / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D042461:Vascular Endothelial Growth Factor A / Q000097:blood
chemical_list: D015415:Biomarkers; D024043:Collagen Type II; D020381:Interleukin-17; D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat