title: [Detection of chromosomal aberrations in multiple myeloma with fluorescence in situ hybridization].
abstract: OBJECTIVE
  To detect of chromosomal abnormalities in multiple myeloma (MM) patients with fluorescence in situ hybridization (FISH).
  
  
  METHODS
  FISH was performed in 20 MM patients using 5 specific DNA probes. The difference in chromosomal abnormalities was compared by FISH and other routine cytogenetic tests.
  
  
  RESULTS
  Eighteen of the 20 patients showed chromosomal abnormalities (90%). The positive rates of t(14q32), del(13q14), dup(1q21), and p53 gene were 65% (13 in 20), 55% (11 in 20), 25% (5 in 20), and 15%(3 in 20), respectively. The abnormal rate of the conventional chromosome examination was 15% only.
  
  
  CONCLUSIONS
  FISH is more sensitive than traditional chromosomal tests and can be used as an index in prognostic evaluation for MM. Del(13q14) and t(14q32) are the most common chromosomal abnormalities in MM patients.
authors.affiliation: Department of Hematology, Xiangya Hospital, Changsha, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Cao
authors.forename: Fangping
authors.initials: F
authors.lastname: Chen
authors.forename: Ye
authors.initials: Y
authors.lastname: Cheng
mesh_terms: D002869:Chromosome Aberrations; D002878:Chromosomes, Human, Pair 1; D002882:Chromosomes, Human, Pair 13; D002883:Chromosomes, Human, Pair 14; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009101:Multiple Myeloma / Q000235:genetics*; D011379:Prognosis