title: Recording high-resolution images of two-dimensional crystals of membrane proteins.
abstract: Principles underlying the recording of high-quality/resolution images of two-dimensional crystals of membrane proteins are discussed in the context of instrumental conditions and operational procedures. A detailed example of low-dose microscope settings is provided along with an overview of a program that implements a computer-aided data acquisition procedure.
authors.affiliation: The WM Keck Advanced Microscopy Laboratory, Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA, USA.
authors.forename: Agustin
authors.initials: A
authors.lastname: Avila-Sakar
authors.forename: Xueming
authors.initials: X
authors.lastname: Li
authors.forename: Shawn Q
authors.initials: SQ
authors.lastname: Zheng
authors.forename: Yifan
authors.initials: Y
authors.lastname: Cheng
mesh_terms: D020285:Cryoelectron Microscopy / Q000295:instrumentation / Q000379:methods*; D004780:Environment, Controlled; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008565:Membrane Proteins / Q000737:chemistry*
chemical_list: D008565:Membrane Proteins