title: A chronic oral exposure of pigs with deoxynivalenol partially prevents the acute effects of lipopolysaccharides on hepatic histopathology and blood clinical chemistry.
abstract: Lipopolysaccharides (LPS), a cell wall component of gram-negative bacteria, and deoxynivalenol (DON), a prevalent Fusarium-derived contaminant of cereal grains, are each reported to have detrimental effects on the liver. A potentiating toxic effect of the combined exposure was reported previously in a mouse model and hepatocytes in vitro, but not in swine as the most DON-susceptible species. Thus, pigs were fed either a control diet (CON) or a Fusarium contaminated diet (DON, 3.1mg DON/kg diet) for 37 days. At day 37 control pigs were infused for 1h either with physiological saline (CON_CON), 100μg/kg BW DON (CON_DON), 7.5μg/kg BW LPS (CON_LPS), or both toxins (CON_DON/LPS) and Fusarium-pigs with saline (DON_CON) or 7.5μg/kg BW LPS (DON_LPS). Blood samples were taken before and after infusion (-30, +30, +60, +120, and +180min) for clinical blood chemistry. Pigs were sacrificed at +195min and liver histopathology was performed. LPS resulted in higher relative liver weight (p<0.05), portal, periportal and acinar inflammation (p<0.05), haemorrhage (p<0.01) and pathological bilirubin levels (CON_CON 1.0μmol/L vs. CON_LPS 5.4μmol/L, CON_DON/LPS 8.3μmol/L; p<0.001). DON feeding alleviated effects of LPS infusion on histopathology and blood chemistry to control levels, whereas DON infusion alone had no impact.
authors.affiliation: Institute of Anatomy, Medical Faculty, Otto-von-Guericke University, 39120 Magdeburg, Germany.
authors.forename: Cassandra
authors.initials: C
authors.lastname: Stanek
authors.forename: Nicole
authors.initials: N
authors.lastname: Reinhardt
authors.forename: Anne-Kathrin
authors.initials: AK
authors.lastname: Diesing
authors.forename: Constanze
authors.initials: C
authors.lastname: Nossol
authors.forename: Stefan
authors.initials: S
authors.lastname: Kahlert
authors.forename: Patricia
authors.initials: P
authors.lastname: Panther
authors.forename: Jeannette
authors.initials: J
authors.lastname: Kluess
authors.forename: Hermann-Josef
authors.initials: HJ
authors.lastname: Rothkötter
authors.forename: Doerthe
authors.initials: D
authors.lastname: Kuester
authors.forename: Bianca
authors.initials: B
authors.lastname: Brosig
authors.forename: Susanne
authors.initials: S
authors.lastname: Kersten
authors.forename: Sven
authors.initials: S
authors.lastname: Dänicke
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001774:Blood Chemical Analysis / Q000662:veterinary; D004032:Diet / Q000662:veterinary; D004333:Drug Administration Routes; D050356:Lipid Metabolism / Q000187:drug effects*; D008055:Lipids / Q000097:blood*; D008070:Lipopolysaccharides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D013552:Swine / Q000097:blood* / Q000378:metabolism; D014255:Trichothecenes / Q000633:toxicity*
chemical_list: D008055:Lipids; D008070:Lipopolysaccharides; D014255:Trichothecenes; C007262:deoxynivalenol