title: Separation and quantification of water buffalo milk protein fractions and genetic variants by RP-HPLC.
abstract: A RP-HPLC method, developed for the separation and quantification of the most common genetic variants of bovine milk proteins, was successfully applied to the analysis of water buffalo milk. All the most common buffalo casein and whey proteins fractions, as well as their genetic variants, were detected and separated simultaneously in 40 min. Purified buffalo proteins were used as calibration standards and a total of 536 individual milk samples were analysed for protein composition. α(S1)-, α(S2)-, βγ-, and κ-casein were 32.2%, 15.8%, 36.5%, and 15.5%, respectively, of total casein content, whereas content of β-Lactoglobulin was approximately 1.3 times as high as that of α-Lactalbumin. The existence of a polymorphism of κ-casein was demonstrated in Mediterranean water buffalo and α(S1)- and κ-casein genetic variants were successfully detected by RP-HPLC.
authors.affiliation: Department of Comparative Biomedicine and Food Science, University of Padova, Viale dell'Università 16, 35020 Legnaro, Padova, Italy. valentina.bonfatti@unipd.it
authors.forename: Valentina
authors.initials: V
authors.lastname: Bonfatti
authors.forename: Mery
authors.initials: M
authors.lastname: Giantin
authors.forename: Roberta
authors.initials: R
authors.lastname: Rostellato
authors.forename: Mauro
authors.initials: M
authors.lastname: Dacasto
authors.forename: Paolo
authors.initials: P
authors.lastname: Carnier
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D005260:Female; D014644:Genetic Variation; D008892:Milk / Q000737:chemistry; D008894:Milk Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D008894:Milk Proteins