title: The protective effect of adipose-derived stem cells against liver injury by trophic molecules.
abstract: BACKGROUND
  In this study we investigated whether adipose-derived stem cells (ADSCs) had any beneficial protective effects on liver injury and regeneration in vivo. Moreover, we examined whether ADSCs protect hepatocytes via trophic molecules.
  
  
  METHODS
  We transplanted ADSCs into mice after 70% hepatectomy and ischemia-reperfusion, and observed liver injury and regeneration after reperfusion. We co-cultured hepatocytes with ADSCs using a Transwell system for 7 d and evaluated the viabilities of hepatocytes and the cytokine levels in the culture medium. Bevacizumab was used to confirm the effect of vascular endothelial growth factor (VEGF) on hepatocytes.
  
  
  RESULTS
  ADSCs improved serum liver function at 6 h after reperfusion in a nonlethal model and stimulated liver regeneration at 24 h after reperfusion in a lethal model. VEGF levels in the culture medium were increased by co-culture ADSCs with hepatocytes. ADSCs improved the viabilities of hepatocytes. The inhibited production of VEGF by bevacizumab did not affect the viability of hepatocytes.
  
  
  CONCLUSIONS
  ADSCs were able to ameliorate liver injury and stimulate liver regeneration in subsequent hepatectomy and ischemia-reperfusion-injured model mice. Furthermore, hepatocytes were protected by the trophic molecules of the ADSCs. However, such protective effects might be provided by mechanisms other than VEGF signaling.
authors.affiliation: Department of Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Saito
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Shimada
authors.forename: Tohru
authors.initials: T
authors.lastname: Utsunomiya
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Ikemoto
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Yamada
authors.forename: Yuji
authors.initials: Y
authors.lastname: Morine
authors.forename: Satoru
authors.initials: S
authors.lastname: Imura
authors.forename: Hiroki
authors.initials: H
authors.lastname: Mori
authors.forename: Koji
authors.initials: K
authors.lastname: Sugimoto
authors.forename: Shuichi
authors.initials: S
authors.lastname: Iwahashi
authors.forename: Michihito
authors.initials: M
authors.lastname: Asanoma
mesh_terms: D017667:Adipocytes / Q000502:physiology*; D000818:Animals; D019610:Cytoprotection; D005260:Female; D008099:Liver / Q000098:blood supply; D008115:Liver Regeneration; D051379:Mice; D008807:Mice, Inbred BALB C; D015427:Reperfusion Injury / Q000517:prevention & control*; D013234:Stem Cells / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000502:physiology*
chemical_list: D042461:Vascular Endothelial Growth Factor A