title: Cultivation of a microalga Chlorella vulgaris using recycled aqueous phase nutrients from hydrothermal carbonization process.
abstract: This study investigated the feasibility of using recovered nutrients from hydrothermal carbonization (HTC) for cultivation of microalga Chlorella vulgaris. Different dilution multiples of 50, 100 and 200 were applied to the recycled process water from HTC and algal growth was compared among these media and a standard growth medium BG-11. Algae achieved a biomass concentration of 0.79 g/L on 50 × process water after 4 days. Algae removed total nitrogen, total phosphorus and chemical oxygen demand by 45.5-59.9%, 85.8-94.6% and 50.0-60.9%, respectively, on differently diluted process water. The fatty acid methyl ester yields for algae grown on the process water were 11.2% (50 ×), 11.2% (100 ×) and 9.7% (200 ×), which were significantly higher than 4.5% for BG-11. In addition, algae cultivated on process water had 18.9% higher carbon and 7.8% lower nitrogen contents than those on BG-11, indicating that they are very suitable as biofuel feedstocks.
authors.affiliation: Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Ave., St. Paul, MN 55108, United States.
authors.forename: Zhenyi
authors.initials: Z
authors.lastname: Du
authors.forename: Bing
authors.initials: B
authors.lastname: Hu
authors.forename: Aimin
authors.initials: A
authors.lastname: Shi
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Ma
authors.forename: Yanling
authors.initials: Y
authors.lastname: Cheng
authors.forename: Paul
authors.initials: P
authors.lastname: Chen
authors.forename: Yuhuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Lin
authors.forename: Roger
authors.initials: R
authors.lastname: Ruan
mesh_terms: D057919:Biological Oxygen Demand Analysis; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D048408:Chlorella vulgaris / Q000187:drug effects / Q000254:growth & development*; D004952:Esters / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000032:analysis; D058086:Microalgae / Q000187:drug effects / Q000254:growth & development*; D009584:Nitrogen / Q000302:isolation & purification / Q000494:pharmacology*; D010758:Phosphorus / Q000302:isolation & purification / Q000494:pharmacology*; D059027:Recycling; D013696:Temperature; D014867:Water
chemical_list: D004952:Esters; D008055:Lipids; D014867:Water; D010758:Phosphorus; D002244:Carbon; D009584:Nitrogen