title: Association of monocyte chemoattractant protein-1 -2518A>G polymorphism with occurrence, severity, and outcome in ischemic stroke.
abstract: Monocyte chemoattractant protein-1 (MCP-1) is implicated in promoting atherosclerotic diseases, including stroke. Therefore, several studies have investigated the association between variants of the MCP-1 gene and risk of atherosclerotic diseases. We sought to determine the occurrence of MCP-1 -2518A>G polymorphism in patients with ischemic stroke (IS), and studied its association with the severity of disease and functional outcome after an acute IS. One hundred and forty-five consecutive patients with first ever IS and 145 age- and sex-matched control subjects were recruited. Stroke severity and functional outcome were assessed on admission and at one month post-stroke, respectively. Genotyping for the MCP-1 -2518A>G polymorphism was performed by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). No significant difference in the frequency of MCP-1 -2518A>G genotypes between IS patients and controls was found, with OR = 0.69 (95 % CI 0.46-1.04, P = 0.08). Moreover, carriage of the G allele was not associated with stroke severity (Scandinavian stroke scale score 33.1 vs. 32.5, respectively, P = 0.71), or poor outcome at 1 month post-stroke (63.9 vs. 59.7 %, respectively, P = 0.61). In conclusion, we were unable to demonstrate a significant association of the MCP-1 -2518A>G gene polymorphism with IS occurrence, severity or functional outcome in a Caucasian population. However, larger studies are necessary to fully elucidate the role of this polymorphism in IS.
authors.affiliation: Laboratory of Pharmacology, Medical School, Democritus University of Thrace, Dragana Campus, 68100, Alexandroupolis, Greece.
authors.forename: Efstathia
authors.initials: E
authors.lastname: Giannakopoulou
authors.forename: Georgia
authors.initials: G
authors.lastname: Ragia
authors.forename: Stella
authors.initials: S
authors.lastname: Marousi
authors.forename: John
authors.initials: J
authors.lastname: Ellul
authors.forename: Vangelis G
authors.initials: VG
authors.lastname: Manolopoulos
authors.forename: Anna
authors.initials: A
authors.lastname: Tavridou
mesh_terms: D000368:Aged; D002545:Brain Ischemia / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics*; D018932:Chemokine CCL2 / Q000235:genetics*; D005260:Female; D006115:Greece; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic; D020521:Stroke / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics*; D044465:White People
chemical_list: D018932:Chemokine CCL2