title: High-resolution imaging of 2D outer membrane protein F crystals by atomic force microscopy.
abstract: In this chapter the methodological bases are provided to achieve subnanometer resolution on two-dimensional (2D) membrane protein crystals by atomic force microscopy (AFM). This is outlined in detail with the example of AFM studies of the outer membrane protein F (OmpF) from the bacterium Escherichia coli (E. coli). We describe in detail the high-resolution imaging of 2D OmpF crystals in aqueous solution and under near-physiological conditions. The topographs of OmpF, and stylus effects and artifacts encountered when imaging by AFM are discussed.
authors.affiliation: Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Fotiadis
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Müller
mesh_terms: D003460:Crystallization; D029968:Escherichia coli Proteins / Q000737:chemistry; D018625:Microscopy, Atomic Force / Q000379:methods*; D018272:Porins / Q000737:chemistry*
chemical_list: D029968:Escherichia coli Proteins; C109237:OmpF protein; D018272:Porins