title: Biomarker responses in caged carp (Cyprinuscarpio) and native collected fish (Leporinus obtusidens) in the Río de la Plata Estuary, Argentina.
abstract: Punta Lara is located in the Río de la Plata estuary near industrial areas contaminated mainly by organic pollutants. In this work, the responses and status of hepatic biomarkers were studied in juvenile carp (Cyprinus carpio) by means of a 21-day field exposure in cages and collection of juvenile native fish (Leporinus obtusidens) at Punta Lara. The analyzed hepatic biomarkers were: enzymatic activity of glutathione-S-transferase (GST), catalase (CAT) and superoxide dismutase (SOD), lipid peroxidation level using the thiobarbituric acid reaction (TBARS), and CYP1A protein expression, condition factor (CF) and liver somatic (LSI) index. Taking into account oxidative stress responses, SOD activity was increased in both species, while CAT was increased in C. carpio and decreased in L. obtusidens; TBARS levels indicated that oxidative damage was possibly exerted only in L. obtusidens. Biotransformation responses mediated by CYP1A were observed in both species, while GST activity was induced mainly in carps. Considering morphometric indices, CF and LSI were significantly increased in carps while CF decreased in native species. The anthropogenic pollution detected in this study in Punta Lara was associated with differences in biomarkers on both fish species, although a different pattern of response was observed.
authors.affiliation: Programa de Ecofisiología Aplicada (PRODEA-INEDES) Departamento de Ciencias Básicas, Universidad Nacional de Luján, Casilla de Correo 221, B6700ZB, Luján, Argentina; Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Buenos Aires, Argentina.
authors.forename: Paola
authors.initials: P
authors.lastname: Scarcia
authors.forename: Gabriela
authors.initials: G
authors.lastname: Calamante
authors.forename: Fernando
authors.initials: F
authors.lastname: de la Torre
mesh_terms: D000818:Animals; D001118:Argentina; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002347:Carps / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D060125:Characiformes / Q000378:metabolism*; D063366:Estuaries; D019015:Geologic Sediments / Q000737:chemistry; D005982:Glutathione Transferase / Q000378:metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism; D018384:Oxidative Stress; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D017392:Thiobarbituric Acid Reactive Substances; D014874:Water Pollutants, Chemical; D002374:Catalase; D001189:Aryl Hydrocarbon Hydroxylases; D013482:Superoxide Dismutase; D005982:Glutathione Transferase
keywords: Cyprinus carpio; Leporinus obtusidens; Río de la Plata estuary; biomarkers; field/cage assay; persistent pollutants