title: In vitro antioxidant effects of Aloe barbadensis Miller extracts and the potential role of these extracts as antidiabetic and antilipidemic agents on streptozotocin-induced type 2 diabetic model rats.
abstract: In this study, the total phenolic and flavonoid contents, the 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging ability and the ferric reducing power (FRAP) of  Aloe vera were measured to determine the antioxidant activity of this species. The in vivo antidiabetic effects of the plant were also investigated using streptozotocin-induced type 2 diabetic model rats that were divided into five groups based on the treatment received:  (1) water (WC); (2) glibenclamide; (3) concentrated gel extract (Gel-C); (4) ethanol (80%) gel extract (Gel-Et); and (5) ethanol (80%) skin extract of Aloe vera (Skin-Et). Skin-Et, which contained the highest level of total phenolics (62.37 &#177; 1.34 mg(gallic acid)/kg) and flavonoids (20.83 &#177; 0.77 mg/kg), exhibited the highest scavenging activity (85.01 &#177; 0.52%) and the greatest reducing power (185.98 &#177; 0.41 &#181;M), indicating that the skin contained the highest level of antioxidants. The oral consumption of Gel-Et for 4 weeks a caused significant reduction in the fasting serum glucose levels of the rats. The rats in the Gel-C-, Gel-Et- and Skin-Et-treated groups experienced a reduction in their total cholesterol levels by 11%, 17% and 25%, respectively and a reduction in their LDL cholesterol levels by 45%, 3% and 69%, respectively. The in vivo experimental antioxidant parameter MDA is strongly correlated with the in vitro antioxidant parameters of flavonoids and polyphenols, namely the DPPH and FRAP values (r = 0.94, 0.92, 0.93, 0.90), thus confirming the antioxidant potential of the Aloe vera extracts.
authors.affiliation: Biomedical Research Group, Department of Pharmacology, Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders (BIRDEM), Dhaka, Bangladesh. rasmo04@yahoo.com
authors.forename: Mohammed
authors.initials: M
authors.lastname: Moniruzzaman
authors.forename: Begum
authors.initials: B
authors.lastname: Rokeya
authors.forename: Sohel
authors.initials: S
authors.lastname: Ahmed
authors.forename: Amrita
authors.initials: A
authors.lastname: Bhowmik
authors.forename: Md Ibrahim
authors.initials: MI
authors.lastname: Khalil
authors.forename: Siew Hua
authors.initials: SH
authors.lastname: Gan
mesh_terms: D000504:Aloe / Q000737:chemistry*; D000818:Animals; D001713:Biphenyl Compounds / Q000737:chemistry; D001786:Blood Glucose; D002784:Cholesterol / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005260:Female; D016166:Free Radical Scavengers / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D005609:Free Radicals / Q000737:chemistry; D007004:Hypoglycemic Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D000960:Hypolipidemic Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D007328:Insulin / Q000097:blood; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008315:Malondialdehyde / Q000097:blood; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D020318:Rats, Long-Evans; D013311:Streptozocin; D014280:Triglycerides / Q000097:blood
chemical_list: D001713:Biphenyl Compounds; D001786:Blood Glucose; D016166:Free Radical Scavengers; D005609:Free Radicals; D007004:Hypoglycemic Agents; D000960:Hypolipidemic Agents; D007328:Insulin; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D010851:Picrates; D010936:Plant Extracts; D014280:Triglycerides; D008315:Malondialdehyde; D013311:Streptozocin; D002784:Cholesterol; C004931:1,1-diphenyl-2-picrylhydrazyl