title: The role of acetylation in the subcellular localization of an oncogenic isoform of translation factor eIF5A.
abstract: Mammalian cells express two isoforms of eIF5A, eIF5A1 and eIF5A2, but little is known about the function of eIF5A2. Here we report that eIF5A2 is reversibly acetylated at lysine-47. HDAC6 and SIRT2 were identified as the enzymes responsible for deacetylating eIF5A2. Analysis using acetylation-deficient mutants indicated that acetylation regulates the subcellular localization of eIF5A2.
authors.affiliation: Chemical Genetics Laboratory, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ishfaq
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Maeta
authors.forename: Satoko
authors.initials: S
authors.lastname: Maeda
authors.forename: Toru
authors.initials: T
authors.lastname: Natsume
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ito
authors.forename: Minoru
authors.initials: M
authors.lastname: Yoshida
mesh_terms: D000107:Acetylation; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism*; D009857:Oncogenes; D010448:Peptide Initiation Factors / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D000097574:Eukaryotic Translation Initiation Factor 5A
chemical_list: D010448:Peptide Initiation Factors; D020033:Protein Isoforms; D016601:RNA-Binding Proteins; D000097574:Eukaryotic Translation Initiation Factor 5A