title: Efficient synthesis of phosphatidylserine in 2-methyltetrahydrofuran.
abstract: 2-Methyltetrahydrofuran has recently been described as a promising and green solvent. Herein, it was successfully used as the reaction medium for enzyme-mediated transphosphatidylation of phosphatidylcholine with L-serine with the aim of phosphatidylserine synthesis for the first time. Our results indicated that as high as 90% yield of phosphatidylserine could be achieved after 12 h combined with no byproduct (phosphatidic acid) forming. The present work accommodated a facilely and efficiently enzymatic strategy for preparing phosphatidylserine, which possessed obvious advantages over the reported processes in terms of high efficiency and environmental friendliness. This work is also a proof-of-concept opening the use of 2-methyltetrahydrofuran in biosynthesis as well.
authors.affiliation: Academy of State Administration of Grain, Beijing 100037, PR China. bioduan@gmail.com
authors.forename: Zhang-Qun
authors.initials: ZQ
authors.lastname: Duan
authors.forename: Fei
authors.initials: F
authors.lastname: Hu
mesh_terms: D000085:Acetates / Q000737:chemistry; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D005663:Furans / Q000737:chemistry / Q000378:metabolism*; D010718:Phosphatidylserines / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D010739:Phospholipase D / Q000378:metabolism; D012694:Serine / Q000378:metabolism; D013696:Temperature
chemical_list: D000085:Acetates; D005663:Furans; D010718:Phosphatidylserines; D012694:Serine; C007650:ethyl acetate; D010739:Phospholipase D; C550584:2-methyltetrahydrofuran