title: Structural organization and classification of cytochrome P450 genes in flax (Linum usitatissimum L.).
abstract: Flax CYPome analysis resulted in the identification of 334 putative cytochrome P450 (CYP450) genes in the cultivated flax genome. Classification of flax CYP450 genes based on the sequence similarity with Arabidopsis orthologs and CYP450 nomenclature, revealed 10 clans representing 44 families and 98 subfamilies. CYP80, CYP83, CYP92, CYP702, CYP705, CYP708, CYP728, CYP729, CYP733 and CYP736 families are absent in the flax genome. The subfamily members exhibited conserved sequences, length of exons and phasing of introns. Similarity search of the genomic resources of wild flax species Linum bienne with CYP450 coding sequences of the cultivated flax, revealed the presence of 127 CYP450 gene orthologs, indicating amplification of novel CYP450 genes in the cultivated flax. Seven families CYP73, 74, 75, 76, 77, 84 and 709, coding for enzymes associated with phenylpropanoid/fatty acid metabolism, showed extensive gene amplification in the flax. About 59% of the flax CYP450 genes were present in the EST libraries.
authors.affiliation: Centre for Plant Molecular Biology, Osmania University, Hyderabad 500007, India.
authors.forename: Peram Ravindra
authors.initials: PR
authors.lastname: Babu
authors.forename: Khareedu Venkateswara
authors.initials: KV
authors.lastname: Rao
authors.forename: Vudem Dashavantha
authors.initials: VD
authors.lastname: Reddy
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D017124:Conserved Sequence; D003577:Cytochrome P-450 Enzyme System / Q000145:classification* / Q000235:genetics*; D005091:Exons; D020224:Expressed Sequence Tags; D019597:Flax / Q000235:genetics*; D005784:Gene Amplification; D018745:Genome, Plant; D007438:Introns; D050356:Lipid Metabolism / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D059467:Transcriptome
chemical_list: D003577:Cytochrome P-450 Enzyme System