title: Bioethanol production using carbohydrate-rich microalgae biomass as feedstock.
abstract: This study aimed to evaluate the potential of using a carbohydrate-rich microalga Chlorella vulgaris FSP-E as feedstock for bioethanol production via various hydrolysis strategies and fermentation processes. Enzymatic hydrolysis of C. vulgaris FSP-E biomass (containing 51% carbohydrate per dry weight) gave a glucose yield of 90.4% (or 0.461 g (g biomass)(-1)). The SHF and SSF processes converted the enzymatic microalgae hydrolysate into ethanol with a 79.9% and 92.3% theoretical yield, respectively. Dilute acidic hydrolysis with 1% sulfuric acid was also very effective in saccharifying C. vulgaris FSP-E biomass, achieving a glucose yield of nearly 93.6% from the microalgal carbohydrates at a starting biomass concentration of 50 g L(-1). Using the acidic hydrolysate of C. vulgaris FSP-E biomass as feedstock, the SHF process produced ethanol at a concentration of 11.7 g L(-1) and an 87.6% theoretical yield. These findings indicate the feasibility of using carbohydrate-producing microalgae as feedstock for fermentative bioethanol production.
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan, ROC.
authors.forename: Shih-Hsin
authors.initials: SH
authors.lastname: Ho
authors.forename: Shu-Wen
authors.initials: SW
authors.lastname: Huang
authors.forename: Chun-Yen
authors.initials: CY
authors.lastname: Chen
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Hasunuma
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
authors.forename: Jo-Shu
authors.initials: JS
authors.lastname: Chang
mesh_terms: D056804:Biofuels / Q000382:microbiology*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002241:Carbohydrates / Q000096:biosynthesis*; D048408:Chlorella vulgaris / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000096:biosynthesis; D005959:Glucosidases / Q000378:metabolism; D006868:Hydrolysis / Q000187:drug effects; D008055:Lipids / Q000032:analysis; D058086:Microalgae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D009566:Nitrates / Q000032:analysis; D009584:Nitrogen / Q000494:pharmacology; D011506:Proteins / Q000032:analysis; D013464:Sulfuric Acids / Q000494:pharmacology; D013997:Time Factors; D017355:Zymomonas / Q000187:drug effects / Q000378:metabolism
chemical_list: D056804:Biofuels; D002241:Carbohydrates; D008055:Lipids; D009566:Nitrates; D011506:Proteins; D013464:Sulfuric Acids; D000431:Ethanol; D005959:Glucosidases; D005947:Glucose; D009584:Nitrogen; C033158:sulfuric acid