title: Characterization of a spontaneously immortalized bovine trabecular meshwork cell line.
abstract: Trabecular meshwork (TM) cells have widely been used as an in vitro model for glaucoma research. However, primary TM cells suffer the disadvantages of limited cell numbers and slow rates of proliferation. We discovered a spontaneously transformed bovine TM (BTM) cell line, BTM-28T. This cell line proliferated rapidly in low-glucose culture medium but also demonstrated contact inhibition in high-glucose culture medium. BTM-28T cells expressed key TM cell markers including α-smooth muscle actin (α-SMA), laminin and collagen IV (col IV). Also, 100 nM dexamethasone (DEX) enhanced the formation of cross-linked actin networks (CLANs) in confluent BTM-28T cell cultures. Transforming growth factor beta 2 (TGFβ2) induced the expression of fibronectin (FN), plasminogen activator inhibitor-1 (PAI-1), and connective tissue growth factor (CTGF) in our cell cultures. This cell line will be helpful to better understand the aqueous humor outflow pathway as related to the pathophysiology of glaucoma.
authors.affiliation: Department of Cell Biology and Anatomy, North Texas Eye Research Institute, University of North Texas Health Science Center, CBH449, 3500 Camp Bowie Blvd, Fort Worth, TX 76107, USA. Weiming.mao@unthsc.edu
authors.forename: Weiming
authors.initials: W
authors.lastname: Mao
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Avani
authors.initials: A
authors.lastname: Mody
authors.forename: Michela
authors.initials: M
authors.lastname: Montecchi-Palmer
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Wordinger
authors.forename: Abbot F
authors.initials: AF
authors.lastname: Clark
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D002417:Cattle; D002452:Cell Count; D002461:Cell Line, Transformed; D049109:Cell Proliferation; D024141:Collagen Type IV / Q000378:metabolism; D055513:Connective Tissue Growth Factor / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D005353:Fibronectins / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D005938:Glucocorticoids / Q000494:pharmacology; D007621:Karyotyping; D007797:Laminin / Q000378:metabolism; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism; D014129:Trabecular Meshwork / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D053781:Transforming Growth Factor beta2 / Q000494:pharmacology
chemical_list: D000199:Actins; D015415:Biomarkers; D024141:Collagen Type IV; D005353:Fibronectins; D005938:Glucocorticoids; D007797:Laminin; D017395:Plasminogen Activator Inhibitor 1; D053781:Transforming Growth Factor beta2; D055513:Connective Tissue Growth Factor; D003907:Dexamethasone