title: Time-dependent release of growth factors from implant surfaces treated with plasma rich in growth factors.
abstract: Plasma rich in growth factors (PRGFs) technology is an autologous platelet-rich plasma approach that provides a pool of growth factors and cytokines that have been shown to increase tissue regeneration and accelerate dental implant osseointegration. In this framework, the spatiotemporal release of growth factors and the establishment of a provisional fibrin matrix are likely to be key aspects governing the stimulation of the early phases of tissue regeneration around implants. We investigated the kinetics of growth factor release at implant surfaces functionalized either with PRGFs or platelet-poor plasma and correlated the results obtained with the morphology of the resulting interfaces. Our main finding is that activation and clot formation favors longer residence times of the growth factors at the interfaces studied, probably due to their retention in the adsorbed fibrin matrix. The concentration of the platelet-derived growth factors above the interfaces becomes negligible after 2-4 days and is significantly higher in the case of activated interfaces than in the case of nonactivated ones, whereas that of the plasmatic hepatocyte growth factor is independent of platelet concentration and activation, and remains significant for up to 9 days. Platelet-rich plasma preparations should be activated to permit growth factor release and thereby facilitate implant surface osseointegration.
authors.affiliation: Biosurfaces Unit, CIC biomaGUNE, Paseo Miramón 182, 20009 San Sebastián, Spain.
authors.forename: María Belén
authors.initials: MB
authors.lastname: Sánchez-Ilárduya
authors.forename: Elodie
authors.initials: E
authors.lastname: Trouche
authors.forename: Ricardo
authors.initials: R
authors.lastname: Tejero
authors.forename: Gorka
authors.initials: G
authors.lastname: Orive
authors.forename: Ilya
authors.initials: I
authors.lastname: Reviakine
authors.forename: Eduardo
authors.initials: E
authors.lastname: Anitua
mesh_terms: D005337:Fibrin / Q000378:metabolism; D017228:Hepatocyte Growth Factor / Q000008:administration & dosage*; D006801:Humans; D010949:Plasma / Q000737:chemistry*; D010982:Platelet-Derived Growth Factor / Q000008:administration & dosage*; D053657:Platelet-Rich Plasma / Q000737:chemistry*; D019736:Prostheses and Implants
chemical_list: D010982:Platelet-Derived Growth Factor; D017228:Hepatocyte Growth Factor; D005337:Fibrin