title: Embryotoxic and genotoxic effects of heavy metals and pesticides on early life stages of Pacific oyster (Crassostrea gigas).
abstract: This study evaluated embryotoxicity and genotoxicity of two dissolved metals copper and cadmium (Cu and Cd) and two pesticides (metolachlor and irgarol) occurring in Arcachon Bay (SW France) in Pacific oyster (Crassostrea gigas) larvae and investigated the relationship between those two endpoints. Embryotoxicity was measured by calculating the percentage of abnormal D-shaped larvae and genotoxicity was evaluated with DNA strand breaks using the comet assay. After 24h exposure, significant increases of the percentage of abnormal D-larvae and the DNA strand breaks were observed from 0.1 μg L⁻¹ for Cu, 10 μg L⁻¹ for Cd and 0.01 μg L⁻¹ for both irgarol and metolachlor in comparison with the controls. A strong positive relationship between embryotoxicity and genotoxicity was recorded for Cu, Cd and metolachlor. The current study suggests that copper, irgarol and metolachlor can induce larval abnormalities and DNA damage in a population of exposed oysters at environmentally relevant concentrations.
authors.affiliation: Univ. Bordeaux, EPOC/LPTC, UMR 5805, F-33400 Talence, France.
authors.forename: Huong
authors.initials: H
authors.lastname: Mai
authors.forename: Jérôme
authors.initials: J
authors.lastname: Cachot
authors.forename: Justine
authors.initials: J
authors.lastname: Brune
authors.forename: Oliver
authors.initials: O
authors.lastname: Geffard
authors.forename: Angel
authors.initials: A
authors.lastname: Belles
authors.forename: Hélène
authors.initials: H
authors.lastname: Budzinski
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Morin
mesh_terms: D000081:Acetamides / Q000633:toxicity; D000818:Animals; D002104:Cadmium / Q000633:toxicity; D020552:Comet Assay; D003300:Copper / Q000633:toxicity; D049896:Crassostrea; D004249:DNA Damage; D004625:Embryo, Nonmammalian / Q000187:drug effects; D005602:France; D007814:Larva / Q000187:drug effects; D019216:Metals, Heavy / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity*; D010575:Pesticides / Q000633:toxicity*; D013723:Teratogens / Q000633:toxicity*; D014227:Triazines / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D000081:Acetamides; D019216:Metals, Heavy; D009153:Mutagens; D010575:Pesticides; D013723:Teratogens; D014227:Triazines; D014874:Water Pollutants, Chemical; D002104:Cadmium; C103341:irgarol 1051; D003300:Copper; C051786:metolachlor