title: Reduced Mycobacterium tuberculosis association with monocytes from diabetes patients that have poor glucose control.
abstract: The re-emerging importance of type 2 diabetes mellitus (DM) to tuberculosis (TB) control is of growing concern, but the basis for this relationship is poorly understood. Given the importance of mononuclear phagocytes for TB control and the reported alterations in monocytes of DM patients, we evaluated whether the initial interaction between both was affected in diabetics. Mycobacterium tuberculosis-naïve individuals with and without DM were group matched by age and gender and the efficiency of M. tuberculosis association (attachment and ingestion) with their monocytes was assessed in the presence of autologous serum. The association of M. tuberculosis with monocytes was significantly lower in diabetics (19.2 ± 6.1) than non-diabetics (27.5 ± 7.9; p = 0.02). Multivariate analysis controlling for host socio demographics, DM characteristics and serum lipids indicated that male gender (p = 0.04) and poorly-controlled DM (high HbA1c and hyperglycemia; p = 0.01) were significantly associated with the lower interaction of M. tuberculosis with monocytes. Serum heat-inactivation reduced the association of M. tuberculosis to similar levels in both study groups (p = 0.69) suggesting alterations in the complement pathway of DM patients. These findings suggest an altered route of entry of the pathogen in DM patients that may influence the downstream activation of signaling pathways in the monocyte and the survival of mycobacteria.
authors.affiliation: Division of Epidemiology, University of Texas Health Science Center at Houston, School of Public Health at Brownsville, 80 Fort Brown, SPH Bldg, Brownsville, TX 78520, USA. Diana.i.gomez@gmail.com
authors.forename: Diana I
authors.initials: DI
authors.lastname: Gomez
authors.forename: Marcel
authors.initials: M
authors.lastname: Twahirwa
authors.forename: Larry S
authors.initials: LS
authors.lastname: Schlesinger
authors.forename: Blanca I
authors.initials: BI
authors.lastname: Restrepo
mesh_terms: D000328:Adult; D003165:Complement System Proteins / Q000276:immunology; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000276:immunology; D005260:Female; D006801:Humans; D006943:Hyperglycemia / Q000150:complications* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology / Q000382:microbiology*; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D009895:Opsonin Proteins / Q000097:blood; D010587:Phagocytosis / Q000276:immunology; D012737:Sex Factors; D015398:Signal Transduction / Q000276:immunology; D014376:Tuberculosis / Q000150:complications* / Q000276:immunology
chemical_list: D009895:Opsonin Proteins; D003165:Complement System Proteins