title: Halopelagius fulvigenes sp. nov., a halophilic archaeon isolated from a lake.
abstract: Two extremely halophilic archaea, designated YIM 94188(T) and YIM 94189, were isolated from Qijiaojing lake in Xinjiang province, north-west China and subjected to taxonomic characterization using a polyphasic approach. The cells of the two strains were coccoid, non-motile and Gram-stain-negative. Colonies were pink-white-pigmented and aerobic. Growth occurred at 10-30% (w/v) NaCl, 20-55 °C and pH 6.0-8.0 (optimum: 20-25% NaCl, 37-42 °C, pH 6.5-7.0). Magnesium was necessary for growth in the range of 0.2-1.2 M. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the two strains belonged to the genus Halopelagius showing 98.5% sequence similarity to the closest phylogenetic neighbour, Halopelagius inordinatus RO5-2(T). In addition, the DNA-DNA hybridization values of strains YIM 94188(T) and YIM 94189 to Halopelagius inordinatus RO5-2(T) were 35.7% and 37.7%, respectively. Polar lipid analyses revealed that the two strains contained phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), sulfated mannosyl glucosyl diether (S-DGD-1) and mannosyl glucosyl diether (DGD-1). The DNA G+C contents of strains YIM 94188(T) and YIM 94189 were 66.3 mol% and 64.6 mol%, respectively. On the basis of physiological and chemotaxonomic data, and phylogenetic analysis, strains YIM 94188(T) and YIM 94189 were classified as representing a novel species in the genus Halopelagius. The name Halopelagius fulvigenes sp. nov. is proposed, with YIM 94188(T) (=CCTCC AB 2010456(T)=JCM 17506(T)) as the type strain.
authors.affiliation: Key Laboratory of Biogeography and Bioresource in Arid Land, CAS, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China.|College of Life Sciences, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Bing-Bing
authors.initials: BB
authors.lastname: Liu
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.|Key Laboratory of Biogeography and Bioresource in Arid Land, CAS, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China.
authors.forename: Shu-Kun
authors.initials: SK
authors.lastname: Tang
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Heng-Lin
authors.initials: HL
authors.lastname: Cui
authors.affiliation: Key Laboratory of Biogeography and Bioresource in Arid Land, CAS, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China.
authors.forename: Yong-Guang
authors.initials: YG
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biogeography and Bioresource in Arid Land, CAS, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Key Laboratory of Biogeography and Bioresource in Arid Land, CAS, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China.
authors.forename: Yuan-Ming
authors.initials: YM
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, Tarim University, Alar 843300, PR China.|College of Life Sciences, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.|Key Laboratory of Biogeography and Bioresource in Arid Land, CAS, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi 830011, PR China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D001482:Base Composition; D002681:China; D019641:DNA, Archaeal / Q000235:genetics; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D060106:Lakes / Q000382:microbiology*; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D019641:DNA, Archaeal; D008055:Lipids; D012336:RNA, Ribosomal, 16S