title: Inhibitory effects of wasabi isothiocyanates on chemical mediator release in RBL-2H3 rat basophilic leukemia cells.
abstract: Wasabi is a plant of Japanese origin. It belongs to the family Brassicaceae and produces various isothiocyanates (ITCs). To clarify the type I allergies inhibited by wasabi ITCs, we investigated the inhibitory effect on chemical mediator release from dinitrophenylated bovine serum albumin (DNP-BSA)-stimulated RBL-2H3 rat basophilic leukemia cells. Allyl ITC (AITC), sec-butyl ITC (s-BuITC), and 3-butenyl ITC (3-BuITC), which have 3 or 4 carbon chains, inhibited histamine release but did not inhibit the release of leukotriene B4 (LTB4) or cysteinyl LTs (CysLTs). 4-Pentenyl ITC (4-PeITC) and 5-hexenyl ITC (5-HeITC), which have 5 or 6 carbon chains and an unsaturated bond at the end, inhibited LTB4 release but did not inhibit the release of histamine or CysLTs. 6-Methylthiohexyl ITC (6-MTITC), 6-methylsulfinylhexyl ITC (6-MSITC), and 6-methylsulfonylhexyl ITC (6-MSFITC), which have a sulfur atom inserted at the end of a 6-carbon chain, inhibited the release of histamine, LTB4, and CysLTs and the elevation in intracellular Ca(2+). These results suggest that wasabi ITCs inhibited type I allergies by inhibiting chemical mediator release and that the inhibitory effects on each chemical mediator were due to differences in the side chain structure of the wasabi ITCs.
authors.affiliation: Kinjirushi Co., Ltd., Nagoya, Aichi, Japan. t-yamada@kinjirushi.co.jp
authors.forename: Tomoe
authors.initials: T
authors.lastname: Yamada-Kato
authors.forename: Masashi
authors.initials: M
authors.lastname: Nagai
authors.forename: Motoko
authors.initials: M
authors.lastname: Ohnishi
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Yoshida
mesh_terms: D000818:Animals; D002417:Cattle; D045744:Cell Line, Tumor; D004140:Dinitrophenols / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects; D017879:Isothiocyanates / Q000494:pharmacology*; D015471:Leukemia, Basophilic, Acute / Q000473:pathology; D007975:Leukotriene B4 / Q000037:antagonists & inhibitors / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D012710:Serum Albumin, Bovine / Q000378:metabolism; D031228:Wasabia / Q000737:chemistry*
chemical_list: C111984:6-methylsulfinylhexyl isothiocyanate; D004140:Dinitrophenols; D017879:Isothiocyanates; D010936:Plant Extracts; C034638:dinitrophenyl-bovine serum albumin; D007975:Leukotriene B4; D012710:Serum Albumin, Bovine; C041942:2,3,4-tri-O-acetylarabinopyranosyl isothiocyanate; C410683:6-methylthiohexyl isothiocyanate