title: Biochemical markers of vascular calcification in elderly hemodialysis patients.
abstract: The increased vascular calcification, cardiovascular morbidity, and mortality in chronic kidney disease (CKD) patients has been associated with disturbances in mineral-bone metabolism. In order to determine markers of the vascular calcification frequently observed in these patients, blood samples of elderly male and female hemodialysis CKD patients were used to measure serum levels of: osteoprotegerin (OPG), total soluble receptor activator of nuclear factor-κB ligand (sRANKL), and fetuin-A by enzyme immunoassay; tartrate-resistant acid phosphatase (TRACP-5b), and bone-specific alkaline phosphatase (BAP) by immunoenzymometric assay; osteocalcin (OC) by ELISA; iPTH by immunoradiometric assay; 25(OH)D(3) and 1,25(OH)(2)D(3), by I(125) radioimmunoassay; and calcium and phosphorus by photometric assay. Serum OPG, BAP, iPTH, phosphorus, and OC levels were higher and serum 25(OH)D(3), 1,25(OH)(2)D(3), and fetuin-A levels lower in both male and female CKD patients than in their respective controls. Our results indicate that the bone formation and resorption parameters are altered in elderly male and female hemodialysis CKD patients. These changes may lead to vascular calcifications and cardiovascular complications, given that elevated OPG and OC levels and reduced fetuin-A levels are associated with cardiovascular events.
authors.affiliation: Vascular Surgery Unit, Sanitas Hospital, Madrid, Spain.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Osorio
authors.forename: Esperanza
authors.initials: E
authors.lastname: Ortega
authors.forename: Jesús M
authors.initials: JM
authors.lastname: Torres
authors.forename: Pilar
authors.initials: P
authors.lastname: Sanchez
authors.forename: Estrella
authors.initials: E
authors.lastname: Ruiz-Requena
mesh_terms: D000135:Acid Phosphatase / Q000097:blood; D000368:Aged; D000469:Alkaline Phosphatase / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002318:Cardiovascular Diseases / Q000097:blood*; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000097:blood; D008297:Male; D008875:Middle Aged; D053244:Osteoprotegerin / Q000097:blood; D053245:RANK Ligand / Q000097:blood; D006435:Renal Dialysis; D051436:Renal Insufficiency, Chronic / Q000097:blood* / Q000378:metabolism / Q000473:pathology; D000071681:Tartrate-Resistant Acid Phosphatase; D061205:Vascular Calcification / Q000097:blood*; D060749:alpha-2-HS-Glycoprotein / Q000032:analysis
chemical_list: C556354:AHSG protein, human; D015415:Biomarkers; D007527:Isoenzymes; D053244:Osteoprotegerin; D053245:RANK Ligand; C505688:TNFSF11 protein, human; D060749:alpha-2-HS-Glycoprotein; D000469:Alkaline Phosphatase; C000605510:ACP5 protein, human; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase