title: MicroRNA-550a acts as a pro-metastatic gene and directly targets cytoplasmic polyadenylation element-binding protein 4 in hepatocellular carcinoma.
abstract: MicroRNAs (miRNAs) are a class of small, non-coding RNA molecules that are often found at chromosomal breakpoints and play a vital role in human cancer. Our previous study found that miR-550a, a frequently amplified miRNA on 7p14.3, was upregulated in hepatocellular carcinoma (HCC). However, the possible functions and molecular mechanisms of miR-550a in HCC remain unknown. In this study, gain-of-function and loss-of-function assays revealed that miR-550a markedly promoted HCC cell migration and invasion. In addition, we discovered that cytoplasmic polyadenylation element binding protein 4 (CPEB4) was a potential target of miR-550a in HCC. Further analyses showed that knockdown of CPEB4 expression significantly facilitated HCC cell migration and invasion, which phenocopied the effects of miR-550a on HCC cells. Moreover, a decrease in CPEB4 expression mediated miR-550a-induced liver cancer cell migration and invasion. Interestingly, CPEB4 is frequently downregulated in HCC, and its expression levels correlate with the overall survival of HCC patients. Together, these results suggested that this newly identified miR-550a-CPEB4 axis may be involved in HCC cell metastasis. Moreover, the expression levels of CPEB4 could be used to predict outcomes in HCC patients. Our findings provide novel potential targets for HCC therapy and prognosis.
authors.affiliation: State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Tian
authors.forename: Linhui
authors.initials: L
authors.lastname: Liang
authors.forename: Jie
authors.initials: J
authors.lastname: Ding
authors.forename: Ruopeng
authors.initials: R
authors.lastname: Zha
authors.forename: Haibing
authors.initials: H
authors.lastname: Shi
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Wang
authors.forename: Shenglin
authors.initials: S
authors.lastname: Huang
authors.forename: Weijie
authors.initials: W
authors.lastname: Guo
authors.forename: Chao
authors.initials: C
authors.lastname: Ge
authors.forename: Taoyang
authors.initials: T
authors.lastname: Chen
authors.forename: Jinjun
authors.initials: J
authors.lastname: Li
authors.forename: Xianghuo
authors.initials: X
authors.lastname: He
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D026723:Polyadenylation; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
chemical_list: C554939:CPEB4 protein, human; D035683:MicroRNAs; D016601:RNA-Binding Proteins