title: Development of a novel fluorophore for real-time biomonitoring system.
abstract: Rapid in-field diagnosis is very important to prevent the outbreak of various infectious and contagious diseases. Highly sensitive and quantitative detection of diseases can be performed using fluorescent immunochemical assay with specific antigen-antibody binding and a good quality fluorophore. This can lead to the development of a small, portable, quantitative biosensor to transmit diagnostic results to a control center in order to systematically prevent disease outbreaks. In this study, we developed a novel fluorophore, coumarin-derived dendrimer, with high emission intensity, strong signal brightness, and high photostability. It is easily coupled with biomolecules and emits strong and stable fluorescence at 590 nm with excitation at 455 nm. Application to fluorescent immunochromatographic test (FICT) showed that the novel coumarin-derived dendrimer bioconjugate could detect antigens at amount as low as 0.1 ng. The clinical results and the spectral characteristics of the novel coumarin-derived dendrimer open, for the first time, the possibility of developing a cost/energy efficient LED-based portable quantitative biosensor for point-of-care (POC) disease diagnosis, which can permit real time monitoring (U-healthcare system) by a disease control center.
authors.affiliation: Zoonosis Research Center, Department of Infection Biology, Wonkwang University College of Medicine, Iksan, Jeonbuk, Republic of Korea.
authors.forename: Hyun-Ok
authors.initials: HO
authors.lastname: Song
authors.forename: Binna
authors.initials: B
authors.lastname: Lee
authors.forename: Ram Prasad
authors.initials: RP
authors.lastname: Bhusal
authors.forename: Byounghun
authors.initials: B
authors.lastname: Park
authors.forename: Kyoungsik
authors.initials: K
authors.lastname: Yu
authors.forename: Chom-Kyu
authors.initials: CK
authors.lastname: Chong
authors.forename: PyoYun
authors.initials: P
authors.lastname: Cho
authors.forename: Sung Yeon
authors.initials: SY
authors.lastname: Kim
authors.forename: Hak Sung
authors.initials: HS
authors.lastname: Kim
authors.forename: Hyun
authors.initials: H
authors.lastname: Park
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods; D003199:Computer Systems; D003374:Coumarins / Q000737:chemistry; D050091:Dendrimers / Q000737:chemistry; D004196:Disease Outbreaks; D004952:Esters / Q000737:chemistry; D005456:Fluorescent Dyes / Q000494:pharmacology; D015658:HIV Infections / Q000175:diagnosis; D006526:Hepatitis C / Q000175:diagnosis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D008288:Malaria / Q000175:diagnosis / Q000469:parasitology; D008956:Models, Chemical; D010966:Plasmodium vivax / Q000378:metabolism; D045663:Quantum Dots; D013053:Spectrophotometry / Q000379:methods; D013388:Succinimides / Q000737:chemistry
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D003374:Coumarins; D050091:Dendrimers; D004952:Esters; D005456:Fluorescent Dyes; D013388:Succinimides; C032620:succinimide; C030123:coumarin