title: Efficient EGFR signaling and dorsal-ventral axis patterning requires syntaxin dependent Gurken trafficking.
abstract: Vesicle trafficking plays a crucial role in the establishment of cell polarity in various cellular contexts, including axis-pattern formation in the developing egg chamber of Drosophila. The EGFR ligand, Gurken (Grk), is first localized at the posterior of young oocytes for anterior-posterior axis formation and later in the dorsal anterior region for induction of the dorsal-ventral (DV) axis, but regulation of Grk localization by membrane trafficking in the oocyte remains poorly understood. Here, we report that Syntaxin 1A (Syx1A) is required for efficient trafficking of Grk protein for DV patterning. We show that Syx1A is associated with the Golgi membrane and is required for the transportation of Grk-containing vesicles along the microtubules to their dorsal anterior destination in the oocyte. Our studies reveal that the Syx1A dependent trafficking of Grk protein is required for efficient EGFR signaling during DV patterning.
authors.affiliation: Department of Biological Science, Florida State University, Tallahassee, FL 32306-4295, USA.
authors.forename: Ai-Guo
authors.initials: AG
authors.lastname: Tian
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Tamori
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Huang
authors.forename: Natalia Toledo
authors.initials: NT
authors.lastname: Melendez
authors.forename: Wu-Min
authors.initials: WM
authors.lastname: Deng
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D019521:Body Patterning; D002454:Cell Differentiation; D019070:Cell Lineage; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D010641:Phenotype; D021381:Protein Transport; D050765:Qa-SNARE Proteins / Q000378:metabolism*; D018010:Receptors, Invertebrate Peptide / Q000378:metabolism*; D015398:Signal Transduction; D016211:Transforming Growth Factor alpha / Q000378:metabolism*; D020691:rab GTP-Binding Proteins / Q000378:metabolism
chemical_list: D015415:Biomarkers; D029721:Drosophila Proteins; D050765:Qa-SNARE Proteins; C094135:Rab6 protein; D018010:Receptors, Invertebrate Peptide; D016211:Transforming Growth Factor alpha; C083730:grk protein, Drosophila; C578392:syntaxin, Drosophila; C066532:Egfr protein, Drosophila; D066246:ErbB Receptors; D020691:rab GTP-Binding Proteins