title: Different Ca²⁺-sensitivities between the EF-hands of T- and L-plastins.
abstract: Plastins are Ca(2+)-regulated actin-bundling proteins, and essential for developing and stabilizing actin cytoskeletons. T-plastin is expressed in epithelial and mesenchymal cells of solid tissues, whereas L-plastin is expressed in mobile cells such as hemopoietic cell lineages and cancer cells. Using various spectroscopic methods, gel-filtration chromatography, and isothermal titration calorimetry, we here demonstrate that the EF-hand motifs of both T- and L-plastin change their structures in response to Ca(2+), but the sensitivity to Ca(2+) is lower in T-plastin than in L-plastin. These results suggest that T-plastin is suitable for maintaining static cytoskeletons, whereas L-plastin is suitable for dynamic rearrangement of cytoskeletons.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Miyakawa
authors.forename: Hiroto
authors.initials: H
authors.lastname: Shinomiya
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Yumoto
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Miyauchi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tanaka
authors.forename: Takao
authors.initials: T
authors.lastname: Ojima
authors.forename: Yusuke S
authors.initials: YS
authors.lastname: Kato
authors.forename: Masaru
authors.initials: M
authors.lastname: Tanokura
mesh_terms: D000595:Amino Acid Sequence; D002118:Calcium / Q000737:chemistry*; D002151:Calorimetry; D002850:Chromatography, Gel; D003599:Cytoskeleton / Q000737:chemistry; D020832:EF Hand Motifs; D006801:Humans; D008562:Membrane Glycoproteins / Q000737:chemistry*; D008840:Microfilament Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D013050:Spectrometry, Fluorescence
chemical_list: D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; C026742:plastin; D002118:Calcium