title: Sustainable production of pectin from lime peel by high hydrostatic pressure treatment.
abstract: The application of high hydrostatic pressure technology for enzymatic extraction of pectin was evaluated. Cellulase and xylanase under five different combinations (cellulase/xylanase: 50/0, 50/25, 50/50, 25/50, and 0/50 U/g lime peel) at ambient pressure, 100 and 200 MPa were used to extract pectin from dried lime peel. Extraction yield, galacturonic acid (GalA) content, average molecular weight (M(w,ave)), intrinsic viscosity [η](w), and degree of esterification (DE) were compared to those parameters obtained for pectins extracted using acid and aqueous processes. Pressure level, type and concentration of enzyme significantly (p<0.05) influenced yield and DE of pectin. Enzyme and high pressure extraction resulted in yields which were significantly (p<0.05) higher than those using acid and aqueous extraction. Although pressure-induced enzymatic treatment improves pectin yield, it does not have any significant effect on M(w,ave) and [η](w) of pectin extracts indicating the potential of high pressure treatment for enzymatic pectin production as a novel and sustainable process.
authors.affiliation: Department of Food Science, Faculty of Life Science, University of Copenhagen, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark.
authors.forename: Mahsa
authors.initials: M
authors.lastname: Naghshineh
authors.forename: Karsten
authors.initials: K
authors.lastname: Olsen
authors.forename: Constantinos A
authors.initials: CA
authors.lastname: Georgiou
mesh_terms: D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D017610:Calcium Compounds / Q000737:chemistry*; D004951:Esterification; D006874:Hydrostatic Pressure; D008970:Molecular Weight; D010087:Oxides / Q000737:chemistry*; D010368:Pectins / Q000737:chemistry*; D014783:Viscosity
chemical_list: D017610:Calcium Compounds; D010087:Oxides; D010368:Pectins; C016538:lime