title: Capillary electrophoresis to quantitate gossypol enantiomers in cotton flower petals and seed.
abstract: Gossypol is a toxic compound that occurs as a mixture of enantiomers in cotton plant tissues including seed and flower petals. The (-)-enantiomer is more toxic to non-ruminant animals. Efforts to breed cottonseed with a low percentage of (-)-gossypol requires determination of the (+)- to (-)-gossypol ratio in seed and flower petals. We report a method to quantitatively determine the total gossypol and percent of its enantiomers in cotton tissues using high performance capillary electrophoresis (HPCE). The method utilizes a borate buffer at pH 9.3 using a capillary with internal diameter of 50μm, effective length of 24.5cm, 15kV and cassette temperature of 15°C. This method provides high accuracy and reproducible results with a limit of detection of the individual enantiomers of less than 36ng/mL providing base line separation in less than 6min.
authors.affiliation: Institute of Bioorganic Chemistry AS RUz, Abdullaev Str., 83 Tashkent, Uzbekistan.
authors.forename: Sergey
authors.initials: S
authors.lastname: Vshivkov
authors.forename: Egor
authors.initials: E
authors.lastname: Pshenichnov
authors.forename: Zamira
authors.initials: Z
authors.lastname: Golubenko
authors.forename: Alik
authors.initials: A
authors.lastname: Akhunov
authors.forename: Shadman
authors.initials: S
authors.lastname: Namazov
authors.forename: Robert D
authors.initials: RD
authors.lastname: Stipanovic
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D035264:Flowers / Q000737:chemistry*; D003368:Gossypium / Q000737:chemistry*; D006072:Gossypol / Q000032:analysis* / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D013696:Temperature
chemical_list: D006072:Gossypol