title: MiR-15b and miR-152 reduce glioma cell invasion and angiogenesis via NRP-2 and MMP-3.
abstract: We tested invasion and angiogenesis related mRNA expression and miRNA profiles of glioma. Genes with mRNA expression that changed significantly were selected to predict possible miRNAs that regulate mRNA expression, and were then matched with miRNA results. NRP-2 with the matching miRNA miR-15b, and MMP-3 with the matching miRNA miR-152 were selected for further study. Luciferase activity assay confirmed that miR-15b and miR-152 attenuate expression of NRP-2 and MMP-3 protein by binding to NRP-2 and MMP-3 transcript, respectively. In vitro invasion assay data showed that miR-15b and miR-152 significantly decreased 9L cell invasiveness. In vitro tube formation assay data showed that miR-15b reduced tube formation. A preliminary pathway study indicated that miR-15b and miR-152 deactivated the MEK-ERK pathway via NRP-2 and MMP-3 in 9L cells, respectively.
authors.affiliation: Department of Neurology, Henry Ford Hospital, Detroit, MI, USA.
authors.forename: Xuguang
authors.initials: X
authors.lastname: Zheng
authors.forename: Michael
authors.initials: M
authors.lastname: Chopp
authors.forename: Yong
authors.initials: Y
authors.lastname: Lu
authors.forename: Benjamin
authors.initials: B
authors.lastname: Buller
authors.forename: Feng
authors.initials: F
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000473:pathology*; D020935:MAP Kinase Signaling System; D019278:Matrix Metalloproteinase 3 / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D009361:Neoplasm Invasiveness; D009389:Neovascularization, Pathologic / Q000235:genetics* / Q000378:metabolism; D039943:Neuropilin-2 / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D059467:Transcriptome
chemical_list: C578788:MIRN152 microRNA, rat; D035683:MicroRNAs; D039943:Neuropilin-2; D012333:RNA, Messenger; C534110:mirn15 microRNA, rat; D048049:Extracellular Signal-Regulated MAP Kinases; D019278:Matrix Metalloproteinase 3