title: Evaluation and optimisation of preparative semi-automated electrophoresis systems for Illumina library preparation.
abstract: Size selection can be a critical step in preparation of next-generation sequencing libraries. Traditional methods employing gel electrophoresis lack reproducibility, are labour intensive, do not scale well and employ hazardous interchelating dyes. In a high-throughput setting, solid-phase reversible immobilisation beads are commonly used for size-selection, but result in quite a broad fragment size range. We have evaluated and optimised the use of two semi-automated preparative DNA electrophoresis systems, the Caliper Labchip XT and the Sage Science Pippin Prep, for size selection of Illumina sequencing libraries.
authors.affiliation: Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire CB10 1SA, UK. mq1@sanger.ac.uk
authors.forename: Michael A
authors.initials: MA
authors.lastname: Quail
authors.forename: Yong
authors.initials: Y
authors.lastname: Gu
authors.forename: Harold
authors.initials: H
authors.lastname: Swerdlow
authors.forename: Matthew
authors.initials: M
authors.lastname: Mayho
mesh_terms: D004247:DNA / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D004586:Electrophoresis / Q000379:methods*; D015723:Gene Library; D023281:Genomics / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D008970:Molecular Weight; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004247:DNA