title: Two-photon optogenetics of dendritic spines and neural circuits.
abstract: We demonstrate a two-photon optogenetic method that generates action potentials in neurons with single-cell precision, using the red-shifted opsin C1V1(T). We applied the method to optically map synaptic circuits in mouse neocortical brain slices and to activate small dendritic regions and individual spines. Using a spatial light modulator, we split the laser beam onto several neurons and performed simultaneous optogenetic activation of selected neurons in three dimensions.
authors.affiliation: Howard Hughes Medical Institute (HHMI), Columbia University, New York, New York, USA.
authors.forename: Adam M
authors.initials: AM
authors.lastname: Packer
authors.forename: Darcy S
authors.initials: DS
authors.lastname: Peterka
authors.forename: Jan J
authors.initials: JJ
authors.lastname: Hirtz
authors.forename: Rohit
authors.initials: R
authors.lastname: Prakash
authors.forename: Karl
authors.initials: K
authors.lastname: Deisseroth
authors.forename: Rafael
authors.initials: R
authors.lastname: Yuste
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D049229:Dendritic Spines / Q000502:physiology*; D051379:Mice; D009474:Neurons / Q000502:physiology*; D055355:Opsins; D062308:Optogenetics; D018408:Patch-Clamp Techniques; D017785:Photons
chemical_list: D055355:Opsins