title: Morphological and molecular characterization of Explanatum explanatum from cattle and buffaloes in Myanmar.
abstract: A robust molecular marker is needed for discrimination of amphistome species, because identification based on morphology alone requires specialized knowledge and techniques. In this study, we performed morphological and molecular characterization of Explanatum explanatum, a species that causes severe liver damage in definitive host species. Fifty-five adult amphistomes were collected from cattle and water buffaloes in Myanmar. Eighteen of the amphistomes, arbitrarily chosen, were morphologically identified as E. explanatum using sagittal sections. All of the 55 amphistome isolates had identical second internal transcribed spacer (ITS2) of ribosomal DNA sequences; these sequences differed at 7 nucleotide sites from those of the closest species, Paramphistomum leydeni. Our data indicate that the ITS2 sequence could be a useful molecular marker for epidemiological studies on E. explanatum.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi-2-13 Inada-cho, Obihiro, Hokkaido 080-8555, Japan. michikawa@obihiro.ac.jp
authors.forename: Madoka
authors.initials: M
authors.lastname: Ichikawa
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kondoh
authors.forename: Saw
authors.initials: S
authors.lastname: Bawn
authors.forename: Ni Ni
authors.initials: NN
authors.lastname: Maw
authors.forename: Lat Lat
authors.initials: LL
authors.lastname: Htun
authors.forename: Myint
authors.initials: M
authors.lastname: Thein
authors.forename: Aung
authors.initials: A
authors.lastname: Gyi
authors.forename: Kyaw
authors.initials: K
authors.lastname: Sunn
authors.forename: Ken
authors.initials: K
authors.lastname: Katakura
authors.forename: Tadashi
authors.initials: T
authors.lastname: Itagaki
mesh_terms: D000818:Animals; D001649:Bile Duct Diseases / Q000469:parasitology / Q000473:pathology / Q000662:veterinary; D001652:Bile Ducts / Q000469:parasitology / Q000473:pathology; D002020:Buffaloes; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology*; D002052:Myanmar / Q000453:epidemiology; D014200:Trematoda / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics*; D014201:Trematode Infections / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*