title: Production of hydrogen peroxide and hydroxyl radical in potato tuber during the necrotrophic phase of hemibiotrophic pathogen Phytophthora infestans infection.
abstract: In this study, evidence is provided on the formation of hydrogen peroxide (H(2)O(2)) and hydroxyl radical (HO) in the potato tuber during the necrotrophic phase of the hemibiotrophic pathogen Phytophthora infestans infection. Using 3,3-diaminobenzidine tetrahydrochloride (DAB) imaging technique, the formation of H(2)O(2) was demonstrated in P. infestans-infected potato tuber. For the first time, HO formation was demonstrated in P. infestans-infected potato tuber using electron paramagnetic resonance (EPR) spectroscopy. An enhancement in spontaneous ultra-weak photon emission indicated the extent of lipid peroxidation in the P. infestans-infected potato tuber. The data presented in this study reveal that the formation of H(2)O(2) and HO in the P. infestans-infected potato tuber is associated with lipid peroxidation. It is proposed here that the ultra-weak photon emission can be used as a non-invasive indicator of the oxidative processes in the quality control at food industry.
authors.affiliation: Department of Biophysics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 11, 783 71 Olomouc, Czech Republic.
authors.forename: Anshu
authors.initials: A
authors.lastname: Rastogi
authors.forename: Pavel
authors.initials: P
authors.lastname: Pospíšil
mesh_terms: D063427:Food Quality; D006861:Hydrogen Peroxide / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000378:metabolism*; D015227:Lipid Peroxidation; D057054:Molecular Imaging; D055750:Phytophthora infestans / Q000254:growth & development / Q000502:physiology*; D010935:Plant Diseases / Q000469:parasitology*; D011198:Solanum tuberosum / Q000378:metabolism* / Q000469:parasitology*
chemical_list: D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide