title: Effects of zinc supplementation on antioxidant status and lipid peroxidation in hemodialysis patients.
abstract: OBJECTIVE
  This study was designed to determine the effects of zinc supplementation on oxidative stress in hemodialysis (HD) patients through evaluating total antioxidant capacity (TAC), whole blood glutathione peroxidase (GSH) level, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) level.
  
  
  METHODS
  Double-blinded randomized controlled trialfrom October 2006 to December 2007 at Tabriz Imam Khomeini Hospital.
  
  
  METHODS
  Sixty-five HD patients were randomly enrolled into 2 groups.
  
  
  METHODS
  Patients received placebo in group A and zinc (100 mg/day) in group B for 2 months. After a washout period for 2 months, the groups were crossed over and the study was continued for an additional 2 months.
  
  
  METHODS
  Serum zinc concentration was measured using atomic absorption spectrophotometry. TAC, GSH level, and SOD activity were determined by commercial enzyme-linked immunosorbent assay kits. MDA level was measured using a thiobarbituric acid method.
  
  
  RESULTS
  The levels of serum zinc, TAC, GSH (P < .001 for all), and SOD activity (P < .001 for group A and P = .003 for group B) significantly increased after zinc supplementation whereas the serum level of MDA decreased after the same period (P = .003 for group A and P < .001 for group B).
  
  
  CONCLUSIONS
  Zinc supplementation for 2 months improved the serum levels of zinc, antioxidant status, and lipid peroxidation in HD patients.
authors.affiliation: Department of Biochemistry, Ardabil University of Medical Science, Ardabil, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Mazani
authors.forename: Hassan
authors.initials: H
authors.lastname: Argani
authors.forename: Nadereh
authors.initials: N
authors.lastname: Rashtchizadeh
authors.forename: Amir
authors.initials: A
authors.lastname: Ghorbanihaghjo
authors.forename: Amir
authors.initials: A
authors.lastname: Hamdi
authors.forename: Mehrdad Asghari
authors.initials: MA
authors.lastname: Estiar
authors.forename: Nariman
authors.initials: N
authors.lastname: Nezami
mesh_terms: D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000032:analysis*; D015992:Body Mass Index; D018592:Cross-Over Studies; D019587:Dietary Supplements; D004311:Double-Blind Method; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects; D006435:Renal Dialysis; D013054:Spectrophotometry, Atomic; D013482:Superoxide Dismutase / Q000097:blood; D015032:Zinc / Q000008:administration & dosage* / Q000097:blood
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D015032:Zinc