title: [Reversing paclitaxel-resistance of SKOV3-TR30 cell line by curcumin].
abstract: OBJECTIVE
  To explore the effects of curcumin on paclitaxel resistance reversal of SKOV3-TR30 cell line and its mechanism.
  
  
  METHODS
  The (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) MTT assay was performed to determine the sensitivity of curcumin-treated SKOV3-TR30 cells to paclitaxel. The cell cycle distribution of SKOV3-TR30 was analyzed by flow cytometry. And the expression level of glycogen synthase kinase-3 (GSK-3) protein was detected by Western blot.
  
  
  RESULTS
  IC(50) of paclitaxel in SKOV3-TR30 decreased with a treatment of curcumin. And the reversal times was 3.0. Flow cytometric analysis of curcumin-treated SKOV3-TR30 cells demonstrated a distinct G(2)-M phase block (78.5 ± 6.4)% after a 12-hour treatment of paclitaxel versus SKOV3-TR30 cells without curcumin. There was a lack of G(2)-M phase arrest (only 27.0% ± 2.9%). The expression of GSK-3 protein in SKOV3-TR30 cells decreased with the 12 and 24-hour treatments of curcumin.
  
  
  CONCLUSIONS
  Curcumin can partially reverse the paclitaxel-resistance of SKOV3-TR30 cells through a down-regulation of GSK-3.
authors.affiliation: Department of Oncology, Zhejiang University & Women's Reproduction & Health Laboratory of Zhejiang Province, Hangzhou, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Qiu
authors.forename: Yun-feng
authors.initials: YF
authors.lastname: Fu
authors.forename: Qi
authors.initials: Q
authors.lastname: Cheng
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Cheng
authors.forename: Xing
authors.initials: X
authors.lastname: Xie
authors.forename: Wei-guo
authors.initials: WG
authors.lastname: Lü
mesh_terms: D045744:Cell Line, Tumor; D003474:Curcumin / Q000494:pharmacology*; D015536:Down-Regulation; D019008:Drug Resistance, Neoplasm; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism; D006801:Humans; D010051:Ovarian Neoplasms / Q000378:metabolism*; D017239:Paclitaxel / Q000494:pharmacology
chemical_list: D038362:Glycogen Synthase Kinase 3; D003474:Curcumin; D017239:Paclitaxel