title: Isolation and genomic characterization of Chaoyang virus strain ROK144 from Aedes vexans nipponii from the Republic of Korea.
abstract: During June 2003, mosquito surveillance was conducted at a US Army installation and a US Military training site 2 km south of the demilitarized zone, Republic of Korea. Mosquitoes were collected using Mosquito Magnets™, sorted to species, and assayed for the presence of arboviruses. From the 3,149 mosquitoes that were sorted into 126 pools, one Aedes vexans nipponii pool (out of 73 pools) tested positive for flavivirus RNA by reverse transcription-PCR. After isolation from C6/36 cell culture supernatant, the viral genome was sequenced and found to be 98.9% related to Chaoyang virus, a potential arthropod-specific flavivirus. This report details the first identification of Chaoyang virus in the Republic of Korea and highlights its relationship to other flaviviruses.
authors.affiliation: Military Infectious Diseases Research Program, Fort Detrick, MD 21702, USA. john.s.lee@amedd.army.mil
authors.forename: John S
authors.initials: JS
authors.lastname: Lee
authors.forename: Nathan D
authors.initials: ND
authors.lastname: Grubaugh
authors.forename: John P
authors.initials: JP
authors.lastname: Kondig
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Turell
authors.forename: Heung-Chul
authors.initials: HC
authors.lastname: Kim
authors.forename: Terry A
authors.initials: TA
authors.lastname: Klein
authors.forename: Monica L
authors.initials: ML
authors.lastname: O'Guinn
mesh_terms: D000330:Aedes / Q000145:classification / Q000821:virology*; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002478:Cells, Cultured; D005416:Flavivirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D023281:Genomics; D007303:Insect Vectors / Q000821:virology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D014709:Vero Cells
chemical_list: D012367:RNA, Viral