title: Functional insights into the core-TFIIH from a comparative survey.
abstract: TFIIH is a eukaryotic complex composed of two subcomplexes, the CAK (Cdk activating kinase) and the core-TFIIH. The core-TFIIH, composed of seven subunits (XPB, XPD, P62, P52, P44, P34, and P8), plays a crucial role in transcription and repair. Here, we performed an extended sequence analysis to establish the accurate phylogenetic distribution of the core-TFIIH in 63 eukaryotic organisms. In spite of the high conservation of the seven subunits at the sequence and genomic levels, the non-enzymatic P8, P34, P52 and P62 are absent from one or a few unicellular species. To gain insight into their respective roles, we undertook a comparative genomic analysis of the whole proteome to identify the gene sets sharing similar presence/absence patterns. While little information was inferred for P8 and P62, our studies confirm the known role of P52 in repair and suggest for the first time the implication of the core TFIIH in mRNA splicing via P34.
authors.affiliation: Laboratoire de Bioinformatique et Génomique Intégratives, Institut de Génétique et de Biologie Moléculaire et Cellulaire (CNRS, INSERM, UDS), BP163, 67404 Illkirch Cedex, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Bedez
authors.forename: Benjamin
authors.initials: B
authors.lastname: Linard
authors.forename: Xavier
authors.initials: X
authors.lastname: Brochet
authors.forename: Raymond
authors.initials: R
authors.lastname: Ripp
authors.forename: Julie D
authors.initials: JD
authors.lastname: Thompson
authors.forename: Dino
authors.initials: D
authors.lastname: Moras
authors.forename: Odile
authors.initials: O
authors.lastname: Lecompte
authors.forename: Olivier
authors.initials: O
authors.lastname: Poch
mesh_terms: D000818:Animals; D018844:Cyclin-Dependent Kinases / Q000235:genetics; D004268:DNA-Binding Proteins; D019143:Evolution, Molecular; D006801:Humans; D046912:Multiprotein Complexes / Q000235:genetics*; D010802:Phylogeny; D021122:Protein Subunits / Q000235:genetics; D051758:Transcription Factor TFIIH / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D046912:Multiprotein Complexes; D021122:Protein Subunits; D051758:Transcription Factor TFIIH; D018844:Cyclin-Dependent Kinases