title: Purification and characterization of a novel antithrombotic peptide from Scolopendra subspinipes mutilans.
abstract: BACKGROUND
  The centipede has been prescribed for the treatment of cardiovascular diseases in Korea, China and other Far Eastern Asian countries for several hundred years.
  
  
  METHODS
  A novel antithrombotic peptide was isolated from Scolopendra subspinipes mutilans using a combination of ultrafiltration, Sephadex G-50 column, Source 15Q anion exchange column and RP-HPLC C18 column.
  
  
  RESULTS
  The molecular mass of the purified peptide is 346Da measured by Electrospray Ionization Mass Spectrometry (ESI-MS). The primary structure of the peptide is Ser-Gln-Leu (SQL) determined by Edman degradation. SQL potently prolonged the activated partial thromboplastin time (aPTT), and inhibited platelet aggregation.
  
  
  CONCLUSIONS
  These results help to clarify the mechanism of the antithrombotic activity of the centipede for effective treatment of cardiovascular and cerebrovascular diseases.
authors.affiliation: School of Life Science & Technology, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, PR China. yikong668@163.com
authors.forename: Yi
authors.initials: Y
authors.lastname: Kong
authors.forename: Shi-Long
authors.initials: SL
authors.lastname: Huang
authors.forename: Yu
authors.initials: Y
authors.lastname: Shao
authors.forename: Shuai
authors.initials: S
authors.lastname: Li
authors.forename: Ji-Fu
authors.initials: JF
authors.lastname: Wei
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001181:Arthropods / Q000737:chemistry*; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008970:Molecular Weight; D010314:Partial Thromboplastin Time / Q000379:methods; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects; D011517:Prothrombin Time / Q000379:methods
chemical_list: D005343:Fibrinolytic Agents; D010455:Peptides