title: A δ38 deletion variant of human transketolase as a model of transketolase-like protein 1 exhibits no enzymatic activity.
abstract: Besides transketolase (TKT), a thiamin-dependent enzyme of the pentose phosphate pathway, the human genome encodes for two closely related transketolase-like proteins, which share a high sequence identity with TKT. Transketolase-like protein 1 (TKTL1) has been implicated in cancerogenesis as its cellular expression levels were reported to directly correlate with invasion efficiency of cancer cells and patient mortality. It has been proposed that TKTL1 exerts its function by catalyzing an unusual enzymatic reaction, a hypothesis that has been the subject of recent controversy. The most striking difference between TKTL1 and TKT is a deletion of 38 consecutive amino acids in the N-terminal domain of the former, which constitute part of the active site in authentic TKT. Our structural and sequence analysis suggested that TKTL1 might not possess transketolase activity. In order to test this hypothesis in the absence of a recombinant expression system for TKTL1 and resilient data on its biochemical properties, we have engineered and biochemically characterized a "pseudo-TKTL1" Δ38 deletion variant of human TKT (TKTΔ38) as a viable model of TKTL1. Although the isolated protein is properly folded under in vitro conditions, both thermal stability as well as stability of the TKT-specific homodimeric assembly are markedly reduced. Circular dichroism and NMR spectroscopic analysis further indicates that TKTΔ38 is unable to bind the thiamin cofactor in a specific manner, even at superphysiological concentrations. No transketolase activity of TKTΔ38 can be detected for conversion of physiological sugar substrates thus arguing against an intrinsically encoded enzymatic function of TKTL1 in tumor cell metabolism.
authors.affiliation: Albrecht-von-Haller-Institute and Göttingen Center for Molecular Biosciences, Department of Bioanalytics, Georg-August-University Göttingen, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Schneider
authors.forename: Stefan
authors.initials: S
authors.lastname: Lüdtke
authors.forename: Kathrin
authors.initials: K
authors.lastname: Schröder-Tittmann
authors.forename: Cindy
authors.initials: C
authors.lastname: Wechsler
authors.forename: Danilo
authors.initials: D
authors.lastname: Meyer
authors.forename: Kai
authors.initials: K
authors.lastname: Tittmann
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003067:Coenzymes / Q000378:metabolism; D004795:Enzyme Stability; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D017384:Sequence Deletion; D014174:Transketolase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D003067:Coenzymes; C505431:TKTL1 protein, human; D014174:Transketolase