title: Stimulation of electro-olfactogram responses in the main olfactory epithelia by airflow depends on the type 3 adenylyl cyclase.
abstract: Cilia of olfactory sensory neurons are the primary sensory organelles for olfaction. The detection of odorants by the main olfactory epithelium (MOE) depends on coupling of odorant receptors to the type 3 adenylyl cyclase (AC3) in olfactory cilia. We monitored the effect of airflow on electro-olfactogram (EOG) responses and found that the MOE of mice can sense mechanical forces generated by airflow. The airflow-sensitive EOG response in the MOE was attenuated when cAMP was increased by odorants or by forskolin suggesting a common mechanism for airflow and odorant detection. In addition, the sensitivity to airflow was significantly impaired in the MOE from AC3(-/-) mice. We conclude that AC3 in the MOE is required for detecting the mechanical force of airflow, which in turn may regulate odorant perception during sniffing.
authors.affiliation: Department of Pharmacology, School of Medicine, School of Public Health, University of Washington, Seattle, Washington 98195-7750, USA.
authors.forename: Xuanmao
authors.initials: X
authors.lastname: Chen
authors.forename: Zhengui
authors.initials: Z
authors.lastname: Xia
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Storm
mesh_terms: D000262:Adenylyl Cyclases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D005260:Female; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009812:Odorants; D009831:Olfactory Mucosa / Q000187:drug effects / Q000378:metabolism*; D055696:Olfactory Perception / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D012903:Smell / Q000187:drug effects / Q000502:physiology*
chemical_list: D000262:Adenylyl Cyclases; C421784:adenylate cyclase 3