title: Expression and regulation of transient receptor potential cation channel, subfamily M, member 2 (TRPM2) in human endometrium.
abstract: To identify estrogen-responsive genes, we previously isolated estrogen receptor (ER)-binding DNA fragments from human genomic DNA using a recombinant ER protein. Six DNA fragments, each including a perfect palindromic estrogen response element (ERE), were obtained. The nucleotide sequence of one of the six fragments (E1 fragment) showed that the ERE of the E1 fragment is located in the 3'-untranslated region (UTR) of transient receptor potential cation channel, subfamily M, member 2 (TRPM2). Here, we confirmed the estrogen-dependent enhancer activity of the ERE of the E1 fragment by chloramphenicol acetyltransferase assay. TRPM2 mRNA expression was investigated in human endometrium, cultured human endometrial stromal cells (ESCs), and cultured human endometrial epithelial cells (EECs) using RT-PCR. Quantitative RT-PCR revealed that TRPM2 mRNA expression in ESCs increased after 17β-estradiol (E2) treatment. This study demonstrated for the first time that TRPM2 is an estrogen-responsive gene expressed in human endometrial cells.
authors.affiliation: Department of Obstetrics and Gynecology, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
authors.forename: Hisahiko
authors.initials: H
authors.lastname: Hiroi
authors.forename: Mikio
authors.initials: M
authors.lastname: Momoeda
authors.forename: Toru
authors.initials: T
authors.lastname: Watanabe
authors.forename: Masanori
authors.initials: M
authors.lastname: Ito
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Ikeda
authors.forename: Ryo
authors.initials: R
authors.lastname: Tsutsumi
authors.forename: Yumi
authors.initials: Y
authors.lastname: Hosokawa
authors.forename: Minako
authors.initials: M
authors.lastname: Koizumi
authors.forename: Fumiko
authors.initials: F
authors.lastname: Zenri
authors.forename: Masami
authors.initials: M
authors.lastname: Muramatsu
authors.forename: Yuji
authors.initials: Y
authors.lastname: Taketani
authors.forename: Satoshi
authors.initials: S
authors.lastname: Inoue
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D001483:Base Sequence; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D004742:Enhancer Elements, Genetic; D004958:Estradiol / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008875:Middle Aged; D011374:Progesterone / Q000502:physiology; D020218:Response Elements; D017154:Stromal Cells / Q000378:metabolism; D050053:TRPM Cation Channels / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D050053:TRPM Cation Channels; C121934:TRPM2 protein, human; D011374:Progesterone; D004958:Estradiol; D006861:Hydrogen Peroxide; D015500:Chloramphenicol O-Acetyltransferase