title: A global transcriptome analysis of a dog model of congestive heart failure with the human genome as a reference.
abstract: BACKGROUND
  The global molecular changes in cardiac tissue during congestive heart failure (CHF) have not been fully examined. Transcriptome analysis with the use of next-generation sequencers is a useful tool for elucidating the pathogenesis of CHF. Although there are some advantages in a dog CHF model, transcriptome analyses in dogs are limited by the relative lack of genomic information.
  
  
  RESULTS
  The transcriptome analysis of hearts from dogs with CHF was conducted with the use of a genome analyzer and the Casava software. The mRNA sequence reads showed alignments with ∼800 of 1,019 genes from the dog reference database. On the other hand, the reads aligned with ∼15,000 of the 21,407 genes in the hg19 human reference database. The correlation of expressed genes was extremely high (r = 0.93; P < .0001) between the dog and human databases. A pathway analysis using the hg19 reference revealed increased expression of p53 pathway-related (P < 10(-10)) and inflammatory interleukin-related (P < 10(-10)) genes in the CHF model.
  
  
  CONCLUSIONS
  The use of the human genome as a reference in global transcriptome analyses of dogs is a useful approach for investigating diseases such as CHF. Such an approach would also be useful for analyzing disease models in other experimental animals.
authors.affiliation: Central Research Laboratory, Shiga University of Medical Science, Otsu, Shiga, Japan. isono@belle.shiga-med.ac.jp
authors.forename: Takahiro
authors.initials: T
authors.lastname: Isono
authors.forename: Takehiro
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Atuyuki
authors.initials: A
authors.lastname: Wada
authors.forename: Masafumi
authors.initials: M
authors.lastname: Suzaki
authors.forename: Tokuhiro
authors.initials: T
authors.lastname: Chano
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030541:Databases, Genetic; D004195:Disease Models, Animal; D004285:Dogs; D020869:Gene Expression Profiling / Q000379:methods*; D015894:Genome, Human / Q000235:genetics*; D006333:Heart Failure / Q000175:diagnosis / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D013045:Species Specificity