title: Using FRET to study RanGTP gradients in live mouse oocytes.
abstract: Oocytes are extremely large cells that have to coordinate accurate chromosome segregation, asymmetric cytoplasm partitioning together with their own development as fertilizable gametes. For this, they undergo both global (cell cycle progression related) and local changes. It is therefore essential to be able to monitor local changes as they take place in live maturing oocytes. We describe here a method to follow RanGTP gradients using FRET technology in vivo.
authors.affiliation: Institut Jacques Monod, CNRS, UMR 7592, Université Paris Diderot, Sorbonne Paris Cité, Paris, France. dumont@ijm.univ-paris-diderot.fr
authors.forename: Julien
authors.initials: J
authors.lastname: Dumont
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Verlhac
mesh_terms: D000818:Animals; D002469:Cell Separation; D002470:Cell Survival; D005260:Female; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D051379:Mice; D057054:Molecular Imaging; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism*; D010053:Ovary / Q000166:cytology; D018075:RNA, Complementary / Q000235:genetics; D020931:ran GTP-Binding Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D018075:RNA, Complementary; D020931:ran GTP-Binding Protein