title: Clinical application of UF-1000i in combination with AX-4280 for the screening test ability of urinary formed elements.
abstract: OBJECTIVE
  To evaluate the screening test ability of UF-1000i combination with AX-4280 for major pathological urinary formed elements and to establish screening rules suitable for our laboratory.
  
  
  METHODS
  A total of 2220 cases of urine samples were collected from out-patients and inpatients. The sample tests were finished within 4 h after collection. The results were verified and corroborated with microscopic tests.
  
  
  RESULTS
  In 'both-negative' group, the negative predictive values for red blood cell, white blood cell and cast were 99.12%, 99.56% and 100%, respectively, and the false negative rates were 0.87%, 0.44% and 0%, respectively. In 'both-positive' group, the positive predictive values for red blood cell, white blood cell and cast were 69.18%, 74.41% and 33.33%, respectively, and the false positive rates were 30.82%, 25.59% and 66.67%, respectively. In 'U positive/A negative' group, the positive predictive values for red blood cell, white blood cell and cast were 32.47%, 23.67% and 8.57%, respectively, and the false positive rates were 67.53%, 76.36% and 91.43%, respectively. In 'U negative/A positive' group, the positive predictive values for red blood cell, white blood cell and cast were 8.18%, 19.50% and 4.67%, respectively, and the false positive rates were 91.82%, 50.50% and 95.24%, respectively.
  
  
  CONCLUSIONS
  When the results of the red blood cell, white blood cell and cast tested by UF-1000i were all negative, and the results of the red blood cell, white blood cell and protein tested by AX-4280 were negative, an automatic report stating 'normal microscopic appearance' was generated, and then the microscopic test rate was reduced by 37.6%. However, if any automated parameter measurement was out of the reference range, corroboration by manual microscopy was advisable.
authors.affiliation: Department of Clinical Laboratory, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China. fengzhao3901@126.com
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.forename: Yi
authors.initials: Y
authors.lastname: Jin
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Chen
authors.forename: Xinyou
authors.initials: X
authors.lastname: Xie
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004912:Erythrocytes / Q000473:pathology; D005188:False Negative Reactions; D005260:Female; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D007297:Inpatients; D007962:Leukocytes / Q000473:pathology; D008297:Male; D008853:Microscopy; D008875:Middle Aged; D010045:Outpatients; D052638:Particulate Matter / Q000652:urine*; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D012016:Reference Values; D016482:Urinalysis / Q000295:instrumentation* / Q000379:methods*; D014556:Urine / Q000166:cytology*; D055815:Young Adult
chemical_list: D052638:Particulate Matter; D011933:Reagent Kits, Diagnostic