title: BMP-2, VEGF and bFGF synergistically promote the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells.
abstract: Mesenchymal stem cells (MSCs) were treated with bone morphogenetic protein-2 (BMP-2), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) dose-dependently and time-dependently. Together they caused a strong synergistic effect on the osteogenic differentiation of MSCs, with lower concentrations of each factor being enough to show the synergistic promotion (50 ng BMP-2/ml, 1 ng VEGF/ml and 10 ng bFGF/ml). When both VEGF and bFGF were added in the early proliferating stage (the first 7 days) and BMP-2 was added in the late differentiation stage (the last 7 days), osteogenic differentiation of MSCs could be enhanced more effectively.
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu, 610064, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Bai
authors.forename: Peipei
authors.initials: P
authors.lastname: Li
authors.forename: Guangfu
authors.initials: G
authors.lastname: Yin
authors.forename: Zhongbing
authors.initials: Z
authors.lastname: Huang
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Liao
authors.forename: Xianchun
authors.initials: X
authors.lastname: Chen
authors.forename: Yadong
authors.initials: Y
authors.lastname: Yao
mesh_terms: D000818:Animals; D001853:Bone Marrow; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004357:Drug Synergism; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051381:Rats; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D055396:Bone Morphogenetic Protein 2; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D005346:Fibroblast Growth Factors