title: Comparison of 3 kidney injury multiplex panels in rats.
abstract: Kidney injury biomarkers have been utilized by pharmaceutical companies as a means to assess the potential of candidate drugs to induce nephrotoxicity. Multiple platforms and assay methods exist, but the comparison of these methods has not been described. Millipore's Kidney Toxicity panel, EMD/Novagen's Widescreen Kidney Toxicity panel, and Meso Scales Kidney Injury panel were selected based on published information. Kidney injury molecule 1, cystatin C, clusterin, and osteopontin were the 4 biomarkers common among all kits tested and the focus of this study. Rats were treated with a low and high dose of para-aminophenol, a known nephrotoxicant, and urine samples were collected and analyzed on the Bio-Plex 200 or MSD's Sector Imager 6000, according to manufacturers specifications. Comparatively, of the 3 kits, Millipore was the most consistent in detecting elevations of 3 out of the 4 biomarkers at both dose levels and indicated time points.
authors.affiliation: Pfizer Global Research and Development, Drug Safety Research and Development, San Diego, CA, USA. annette.john-baptiste@pfizer.com
authors.forename: Annette
authors.initials: A
authors.lastname: John-Baptiste
authors.forename: Allison
authors.initials: A
authors.lastname: Vitsky
authors.forename: Frederick
authors.initials: F
authors.lastname: Sace
authors.forename: Qing
authors.initials: Q
authors.lastname: Zong
authors.forename: Mira
authors.initials: M
authors.lastname: Ko
authors.forename: Rolla
authors.initials: R
authors.lastname: Yafawi
authors.forename: Ling
authors.initials: L
authors.lastname: Liu
mesh_terms: D058186:Acute Kidney Injury / Q000209:etiology / Q000652:urine*; D000627:Aminophenols / Q000633:toxicity; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D051152:Clusterin / Q000378:metabolism*; D055316:Cystatin C / Q000378:metabolism*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D000072596:Hepatitis A Virus Cellular Receptor 1; D007674:Kidney Diseases; D008297:Male; D008562:Membrane Glycoproteins; D009153:Mutagens / Q000633:toxicity; D053495:Osteopontin / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011933:Reagent Kits, Diagnostic; D011991:Receptors, Virus; D015262:Xenobiotics / Q000633:toxicity*
chemical_list: D000627:Aminophenols; D015415:Biomarkers; D015815:Cell Adhesion Molecules; D051152:Clusterin; D055316:Cystatin C; D000072596:Hepatitis A Virus Cellular Receptor 1; D008562:Membrane Glycoproteins; D009153:Mutagens; D053495:Osteopontin; D011933:Reagent Kits, Diagnostic; D011991:Receptors, Virus; D015262:Xenobiotics; C491143:HAVCR1 protein, human; C110936:Havcr1 protein, rat; C026729:4-aminophenol