title: Isolation, characterization and antifungal activity of proteinase inhibitors from Capsicum chinense Jacq. Seeds.
abstract: Capsicum species belong to the Solanaceae family and have great social, economic and agronomical significance. The present research presents data on the isolation and characterization of Capsicum chinense Jacq. peptides which were scrutinized in relation to their toxicity towards a diverse set of yeast species. The protein extract was separated with C18 reverse-phase chromatography in high performance liquid chromatography, resulting in three different peptide enriched fractions (PEFs) termed PEF1, PEF2 and PEF3. Tricine-SDS-PAGE of the PEF2 revealed peptides with molecular masses of approximately 5.0 and 8.5 kDa. These PEFs also exhibited strong antifungal activity against different yeasts. In the presence of the PEF2, Candida tropicalis exhibited morphological changes, including cellular agglomeration and formation of pseudohyphae. Determined N-terminal sequences of PEF2 and PEF3 were proven to be highly homologous to serine proteinase inhibitors, when analysed by comparative database sequence tools. For this reason were performed protease inhibitory activity assay. The PEFs displayed high inhibitory activity against trypsin and low inhibitory activity against chymotrypsin. PEF2 and PEF3 were considerably unsusceptible to a broad interval of pH and temperatures. Due to the myriad of application of Proteinase inhibitors (PIs) in fields ranging from plant protection against pathogens and pests to medicine such as in cancer and virus replication inhibition, the discovery of new PIs with new properties are of great interest.
authors.affiliation: Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, 28015-602, Brazil.
authors.forename: Germana Bueno
authors.initials: GB
authors.lastname: Dias
authors.forename: Valdirene Moreira
authors.initials: VM
authors.lastname: Gomes
authors.forename: Umberto Zottich
authors.initials: UZ
authors.lastname: Pereira
authors.forename: Suzanna F Ferreira
authors.initials: SF
authors.lastname: Ribeiro
authors.forename: André O
authors.initials: AO
authors.lastname: Carvalho
authors.forename: Rosana
authors.initials: R
authors.lastname: Rodrigues
authors.forename: Olga L Tavares
authors.initials: OL
authors.lastname: Machado
authors.forename: Kátia Valevski Sales
authors.initials: KV
authors.lastname: Fernandes
authors.forename: André Teixeira S
authors.initials: AT
authors.lastname: Ferreira
authors.forename: Jonas
authors.initials: J
authors.lastname: Perales
authors.forename: Maura
authors.initials: M
authors.lastname: Da Cunha
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D002212:Capsicum / Q000737:chemistry*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D011480:Protease Inhibitors / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry*; D015003:Yeasts / Q000187:drug effects / Q000254:growth & development
chemical_list: D000935:Antifungal Agents; D010455:Peptides; D011480:Protease Inhibitors