title: Characterization of EstCOo8 and EstC34, intracellular esterases, from the wine-associated lactic acid bacteria Oenococcus oeni and Lactobacillus hilgardii.
abstract: OBJECTIVE
  To clone and characterize two related intracellular esterases from Oenococcus oeni and Lactobacillus hilgardii under wine-like conditions.
  
  
  RESULTS
  The published genome sequences for O. oeni and Lact. hilgardii were used to identify, clone and purify putative esterase genes from these species designated EstCOo8 and EstC34, respectively. Both esterases are members of family V of lipolytic enzymes. However, EstC34 contains an SGSLG nucleophilic elbow structural motif instead of the usual GGSLG motif which is conserved in other lactic acid bacteria. Both esterases exhibited greatest specificity for C(2) -C(4) pNP-linked substrates and retained activity under wine-like conditions. EstCOo8 had an optimum temperature, pH, and ethanol concentration of 40°C, 5.5 and 6% (v/v), respectively. Whereas EstC34 had an optimum temperature, pH and ethanol concentration of 50°C, 5.0 and 10% (v/v), respectively.
  
  
  CONCLUSIONS
  Both esterases were stable and retained activity under conditions that would be encountered in wine. They have the potential to reduce short-chain ethyl esters such as ethyl acetate.
  
  
  CONCLUSIONS
  This study provides information that might help improve the performance of LAB during malolactic fermentation in wine in the future, either by strain selection, optimization or direct enzyme addition.
authors.affiliation: School of Agriculture, Food and Wine, The University of Adelaide, Glen Osmond, SA, Australia.
authors.forename: K M
authors.initials: KM
authors.lastname: Sumby
authors.forename: P R
authors.initials: PR
authors.lastname: Grbin
authors.forename: V
authors.initials: V
authors.lastname: Jiranek
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D004950:Esterases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D007778:Lactobacillus / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D056585:Oenococcus / Q000201:enzymology* / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014920:Wine / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D004950:Esterases