title: High-resolution structure of AKR1a4 in the apo form and its interaction with ligands.
abstract: Aldo-keto reductase 1a4 (AKR1a4; EC 1.1.1.2) is the mouse orthologue of human aldehyde reductase (AKR1a1), the founding member of the AKR family. As an NADPH-dependent enzyme, AKR1a4 catalyses the conversion of D-glucuronate to L-gulonate. AKR1a4 is involved in ascorbate biosynthesis in mice, but has also recently been found to interact with SMAR1, providing a novel mechanism of ROS regulation by ATM. Here, the crystal structure of AKR1a4 in its apo form at 1.64 Å resolution as well as the characterization of the binding of AKR1a4 to NADPH and P44, a peptide derived from SMAR1, is presented.
authors.affiliation: Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario K7L 3N6, Canada.
authors.forename: Frédérick
authors.initials: F
authors.lastname: Faucher
authors.forename: Zongchao
authors.initials: Z
authors.lastname: Jia
mesh_terms: D000449:Aldehyde Reductase / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D001051:Apoenzymes / Q000737:chemistry; D002151:Calorimetry; D018797:Cell Cycle Proteins / Q000737:chemistry; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins / Q000737:chemistry; D008024:Ligands; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D040681:Structural Homology, Protein
chemical_list: D001051:Apoenzymes; C485952:Banp protein, mouse; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D008024:Ligands; D009687:Nuclear Proteins; D010446:Peptide Fragments; C000627720:Akr1a1 protein, mouse; D000449:Aldehyde Reductase