title: GC/MS analysis of volatiles obtained by headspace solid-phase microextraction and simultaneous-distillation extraction from Rabdosia serra (MAXIM.) HARA leaf and stem.
abstract: Volatiles in Rabdosia serra were investigated by headspace solid phase microextraction (HS-SPME) and simultaneous-distillation extraction (SDE). The HS-SPME technique was previously evaluated to optimise sampling conditions. A total of 56 and 48 compounds including alcohols, aldehydes, hydrocarbons, ketones, carboxylic acid, ester, and aromatics were identified in leaf and stem by optimised HS-SPME method (CAR/PDMS fibre; incubation time, 10 min; extraction temperature, 50°C; extraction time, 40 min), respectively. 1-Octen-3-ol and (2E)-hexenal had significant contribution to R. serra aroma. Cluster analysis indicated that leaf and stem exhibited different volatile diversity. Air drying was favourable for the retention of the volatiles, while freeze- and sun-drying led to the loss of volatiles. SDE method preferred to the analysis of compounds with low volatility including fatty acids and esters. HS-SPME was a useful technique for the analysis of readily volatile components for the characteristics of R. serra aroma.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Lianzhu
authors.initials: L
authors.lastname: Lin
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Zhuang
authors.forename: Fenfen
authors.initials: F
authors.lastname: Lei
authors.forename: Bao
authors.initials: B
authors.lastname: Yang
authors.forename: Mouming
authors.initials: M
authors.lastname: Zhao
mesh_terms: D057168:Distillation / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D037602:Isodon / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D052617:Solid Phase Microextraction / Q000379:methods*; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000302:isolation & purification*
chemical_list: D010936:Plant Extracts; D055549:Volatile Organic Compounds