title: Structural bioinformatics of the general transcription factor TFIID.
abstract: The Transcription Factor IID is a large macromolecular complex composed of the TATA-box binding protein (TBP) and a group of 13-14 conserved TBP-associated factors (TAFs). TAFs are known to regulate transcription at various levels - mediating transcription via interaction with activators, histone modifications; recognition and binding to promoters; acting as a platform for other Transcription Factors and RNA polymerase II. Despite numerous previous studies of the TFIID complex, the knowledge concerning the structure of its components, and thus the exact mechanism of its function, remains undetermined. To carry out an in-depth analysis of TFIID we performed the structural bioinformatic analysis of the TFIID complex. The sequence identity and similarity of 13.74% and 37.56%, respectively (calculated with PAM250 matrix) between M1 aminopeptidase protein and TAF2 and the high similarity of their putative secondary structures allowed us to model a large part of the TAF2 structure. The sequence analysis enabled the mapping of previously not fully characterized structural domains in well-studied TAF proteins (including the full histone domains of TAF4 and 12 or TAF3 and 8). In this study we provided detailed structural models for all the elements of human analyzed in the context of TFIID activity, along with indications of structural alterations within TFIID in various animal model species.
authors.affiliation: Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Laboratory of Bioinformatics and Biostatistics, WK Roentgena 5, 02-781 Warsaw, Poland.
authors.forename: Maja
authors.initials: M
authors.lastname: Malkowska
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kokoszynska
authors.forename: Leszek
authors.initials: L
authors.lastname: Rychlewski
authors.forename: Lucjan
authors.initials: L
authors.lastname: Wyrwicz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019295:Computational Biology / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D035362:Transcription Factor TFIID / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D035362:Transcription Factor TFIID