title: Nitric oxide mediated effects on reproductive toxicity caused by carbon disulfide in male rats.
abstract: This study investigated nitric oxide (NO) mediation of carbon disulfide (CS(2)) toxicity that compromised male rat spermatogenesis and endocrine function. Rats were exposed to multiple levels of CS(2) concentration (0, 50, 250, 1250 mg/m(3)). A 1250 mg/m(3) CS(2)+sodium nitroprusside (SNP) group and a 1250 mg/m(3) CS(2)+NG-monomethyl-L-arginine (L-NMMA) group were established to explore the role of NO in mediating CS(2) toxicity. NO concentrations, NO synthase (NOS) activity, and sex hormone levels were measured, and sperm characteristics were observed and analyzed. Our data show that CS(2) exposure decreased: NOS activity; tissue NO concentrations; serum levels of gonadotropin-releasing hormones, luteinizing hormones, and testosterone; and sperm count and activity. In contrast, increased serum follicle-stimulating hormone concentrations and teratospermia were observed with CS(2) exposure. SNP reduced some of the toxic effects of CS(2), while L-NMMA treatment showed no effect. The results suggests that NO mediates compromised reproductive system function caused by CS(2) exposure.
authors.affiliation: MOE Key Lab of Environment and Health, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, Hubei, PR China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Huang
authors.forename: Yijun
authors.initials: Y
authors.lastname: Zhou
authors.forename: Jiying
authors.initials: J
authors.lastname: Ma
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Jiajia
authors.initials: J
authors.lastname: Ji
authors.forename: Qing
authors.initials: Q
authors.lastname: Ding
authors.forename: Guoyuan
authors.initials: G
authors.lastname: Chen
mesh_terms: D000818:Animals; D002246:Carbon Disulfide / Q000633:toxicity*; D052244:Endocrine Disruptors / Q000633:toxicity; D004702:Endocrine Glands / Q000187:drug effects / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology*; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism; D015386:Hazardous Substances / Q000633:toxicity*; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D009599:Nitroprusside / Q000494:pharmacology; D010902:Pituitary Gland / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012098:Reproduction / Q000187:drug effects*; D013091:Spermatogenesis / Q000187:drug effects; D013737:Testis / Q000378:metabolism; D019323:omega-N-Methylarginine / Q000494:pharmacology
chemical_list: D052244:Endocrine Disruptors; D016166:Free Radical Scavengers; D015386:Hazardous Substances; D009599:Nitroprusside; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D007987:Gonadotropin-Releasing Hormone; D019001:Nitric Oxide Synthase; D002246:Carbon Disulfide