title: [Effect and mechanism of gastrodin in relaxing isolated thoracic aorta rings in rats].
abstract: OBJECTIVE
  To investigate the effect of gastrodin in relaxing isolated thoracic aorta rings in rats and discuss its possible mechanism.
  
  
  METHODS
  Isotonic tension of isolated thoracic aortic rings in rats with norepineprine (NE) and KCl was recorded to observe the vasodilatory effect of gastrodin and the influence of various drugs on it.
  
  
  RESULTS
  Gastrodin had the effect in relaxing thoracic aortas with or without endothelium, and there was no significant difference. NG-nitro-L-argininemethylester (L-NAME, 1 x 10(-4) mol x L(-1)), methylene blue (MB, 1 x 10(-5) mol x L(-1)), indomethacin (INDO, 1 x 10(-5) mol x L(-1)) had no effect on the vasodilation action of gastrodin on thoracic aortas precontracted by NE. 4-aminopyrimide (4-AP, 1 x 10(-4) mol x L(-1)), tetrathylamonium (TEA, 1 x 10(-3) mol x L(-1)), BaCl2 (1 x 10(-4) mol x L(-1)) and glibenclamide (Gli, 1 x 10(-5) mol x L(-1)) could inhibit gastrodin's effect in relaxing thoracic aorta rings. In the absence of Ca2+, pre-incubated gastrodin showed a notable inhibitory effect in relaxing NE contraction.
  
  
  CONCLUSIONS
  Gastrodin shows a dose-dependent and endothelium-independent effect in relaxing rat isolated thoracic aorta rings. The mechanism is related to K+ channel, inhibition of release of Ca+ stored in endoplasmic reticulum of vascular smooth muscle cells and inflow of external calcium Ca2+.
authors.affiliation: China Pharmaceutical University, Nanjing 210009, China. zhangyingqiao2006@126.com
authors.forename: Yingqiao
authors.initials: Y
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Yu
authors.forename: Jiyang
authors.initials: J
authors.lastname: Xu
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Bian
authors.forename: Jianliang
authors.initials: J
authors.lastname: Xu
authors.forename: Yamei
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects* / Q000502:physiology; D001592:Benzyl Alcohols / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D004730:Endothelium, Vascular / Q000502:physiology; D005960:Glucosides / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014664:Vasodilation / Q000187:drug effects*
chemical_list: D001592:Benzyl Alcohols; D005960:Glucosides; C045345:gastrodin; D002118:Calcium; D009638:Norepinephrine