title: Lovastatin production by Aspergillus terreus using agro-biomass as substrate in solid state fermentation.
abstract: Ability of two strains of Aspergillus terreus (ATCC 74135 and ATCC 20542) for production of lovastatin in solid state fermentation (SSF) using rice straw (RS) and oil palm frond (OPF) was investigated. Results showed that RS is a better substrate for production of lovastatin in SSF. Maximum production of lovastatin has been obtained using A. terreus ATCC 74135 and RS as substrate without additional nitrogen source (157.07 mg/kg dry matter (DM)). Although additional nitrogen source has no benefit effect on enhancing the lovastatin production using RS substrate, it improved the lovastatin production using OPF with maximum production of 70.17 and 63.76 mg/kg DM for A. terreus ATCC 20542 and A. terreus ATCC 74135, respectively (soybean meal as nitrogen source). Incubation temperature, moisture content, and particle size had shown significant effect on lovastatin production (P < 0.01) and inoculums size and pH had no significant effect on lovastatin production (P > 0.05). Results also have shown that pH 6, 25°C incubation temperature, 1.4 to 2 mm particle size, 50% initial moisture content, and 8 days fermentation time are the best conditions for lovastatin production in SSF. Maximum production of lovastatin using optimized condition was 175.85 and 260.85 mg/kg DM for A. terreus ATCC 20542 and ATCC 74135, respectively, using RS as substrate.
authors.affiliation: Laboratory of Industrial Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Malaysia.
authors.forename: Mohammad Faseleh
authors.initials: MF
authors.lastname: Jahromi
authors.forename: Juan Boo
authors.initials: JB
authors.lastname: Liang
authors.forename: Yin Wan
authors.initials: YW
authors.lastname: Ho
authors.forename: Rosfarizan
authors.initials: R
authors.lastname: Mohamad
authors.forename: Yong Meng
authors.initials: YM
authors.lastname: Goh
authors.forename: Parisa
authors.initials: P
authors.lastname: Shokryazdan
mesh_terms: D000383:Agriculture; D001230:Aspergillus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D018533:Biomass; D053898:Biosynthetic Pathways / Q000187:drug effects; D001709:Biotechnology / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation / Q000187:drug effects; D006813:Humidity; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008031:Lignin / Q000378:metabolism; D008148:Lovastatin / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry; D008903:Minerals / Q000494:pharmacology; D009584:Nitrogen / Q000494:pharmacology; D012275:Oryza / Q000187:drug effects / Q000378:metabolism*; D000073878:Palm Oil; D010316:Particle Size; D010938:Plant Oils / Q000378:metabolism*; D012996:Solutions; D013379:Substrate Specificity / Q000187:drug effects; D013696:Temperature; D013997:Time Factors
chemical_list: D008903:Minerals; D010938:Plant Oils; D012996:Solutions; C036909:lignocellulose; D000073878:Palm Oil; D008031:Lignin; D008148:Lovastatin; D009584:Nitrogen