title: [Construction of a recombinant stable Ba/F3 cell strain containing Tpr-Met].
abstract: OBJECTIVE
  To construct a stable cell strain encoding tumor-associated fused gene which expresses oncoprotein Tpr-Met.
  
  
  METHODS
  We transfected Tpr-Met vector into Ba/F3 cells and screened the cell strain stably expressing Tpr-Met. The interleukin 3 (IL-3) independent proliferation of the cells was measured using the MTS assay. The expression of Tpr-Met,  the activity of downstream signal transduction pathway and SU11274-induced inhibition of the signal pathway were investigated by Western blotting.
  
  
  RESULTS
  We obtained a Ba/F3 cell strain stably expressing Tpr-Met. The cells presented IL-3 independent proliferation,  suggesting a malignant transformation of the cell line. In Tpr-Met transformed Ba/F3 cells,  the phosphorylation of Met and ERK were enhanced;  however,  specific c-Met inhibitor SU11274 suppressed the cell proliferation and c-Met phosphorylation.
  
  
  CONCLUSIONS
  Tpr-Met transformed Ba/F3 strain has been successfully constructed.
authors.affiliation: Department of Biochemistry and Molecular Biology, Anhui Medical University, Hefei, China.
authors.forename: Ling-fei
authors.initials: LF
authors.lastname: Shu
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Yi-qun
authors.initials: YQ
authors.lastname: Zhan
authors.forename: Wang-xiang
authors.initials: WX
authors.lastname: Xu
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Yang
authors.forename: Chang-yan
authors.initials: CY
authors.lastname: Li
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D007211:Indoles / Q000494:pharmacology; D007377:Interleukin-3 / Q000494:pharmacology; D051379:Mice; D050490:Oncogene Protein tpr-met / Q000235:genetics*; D010766:Phosphorylation; D010879:Piperazines / Q000494:pharmacology; D015398:Signal Transduction; D013449:Sulfonamides / Q000494:pharmacology; D014162:Transfection
chemical_list: C478479:((3Z)-N-(3-chlorophenyl)-3-((3,5-dimethyl-4-((4-methylpiperazin-1-yl)carbonyl)-1H-pyrrol-2-yl)methylene)-N-methyl-2-oxo-2,3-dihydro-1H-indole-5-sulfonamide); D007211:Indoles; D007377:Interleukin-3; D050490:Oncogene Protein tpr-met; D010879:Piperazines; D013449:Sulfonamides