title: Effects of montelukast and zileuton on testicular torsion/detorsion injury in rats.
abstract: The aim of this study was to evaluate and compare the effects of 5-lipoxygenase enzyme (5-LO) inhibitor zileuton and cysteinyl leukotriene receptor (CysLT1R) antagonist montelukast in testicular torsion/detorsion (T/D) injury model in rats. Rats were anaesthetised with 75 mg kg(-1) ketamine hydrochloride and 8 mg kg(-1) xylazine intraperitoneal before the operation. Torsion was created by rotating the right testis 720° clockwise and maintained by fixing the testis. The rats were treated with CysLT1R antagonist montelukast (10 mg kg(-1); i.p.), 5-LO inhibitor zileuton (3 mg kg(-1); i.p.), and vehicle, at 30 min prior detorsion. After 1 h of torsion, the testis was counter-rotated to the natural position and replaced into the scrotum. Malondialdehyde (MDA) level was measured in testicular tissue after 3 h of reperfusion. Histological examination was performed after 24 h of reperfusion. T/D caused a significant increase in MDA level and histopathological injury in testes. Montelukast and zileuton treatments prevented the T/D-induced augmentation in MDA levels. Only zileuton treatment significantly reduced the T/D-induced histopathological injury. In this study, we demonstrated for the first time that zileuton had protective effects on testicular T/D injury. We have also found that zileuton is more effective than montelukast on histopathological injury.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Bulent Ecevit University, Zonguldak, Turkey.
authors.forename: F
authors.initials: F
authors.lastname: Isikdemir
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Bulent Ecevit University, Zonguldak, Turkey.
authors.forename: Z
authors.initials: Z
authors.lastname: Kurcer
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Bulent Ecevit University, Zonguldak, Turkey.
authors.forename: G O
authors.initials: GO
authors.lastname: Dengiz
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Bulent Ecevit University, Zonguldak, Turkey.
authors.forename: E Y
authors.initials: EY
authors.lastname: Sipahi
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Bulent Ecevit University, Zonguldak, Turkey.
authors.forename: Z N
authors.initials: ZN
authors.lastname: Banoglu
authors.affiliation: Department of Pathology, Selcuklu Faculty of Medicine, Selcuk University, Konya, Turkey.
authors.forename: F
authors.initials: F
authors.lastname: Baba
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Bulent Ecevit University, Zonguldak, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Acikgoz
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Bulent Ecevit University, Zonguldak, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Kelek
mesh_terms: D000085:Acetates / Q000627:therapeutic use*; D000818:Animals; D003521:Cyclopropanes; D006918:Hydroxyurea / Q000031:analogs & derivatives* / Q000627:therapeutic use; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D011804:Quinolines / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D013086:Spermatic Cord Torsion / Q000188:drug therapy* / Q000378:metabolism; D013440:Sulfides
chemical_list: D000085:Acetates; D003521:Cyclopropanes; D011804:Quinolines; D013440:Sulfides; D008315:Malondialdehyde; C093875:montelukast; C063449:zileuton; D006918:Hydroxyurea
keywords: Montelukast; testis; torsion/detorsion; zileuton