title: Immune-related gene expression in nurse honey bees (Apis mellifera) exposed to synthetic acaricides.
abstract: The mite Varroa destructor is an ectoparasite affecting honey bees worldwide. Synthetic acaricides have been among the principal tools available to beekeepers for its control, although several studies have shown its negative effects on honey bee physiology. Recent research suggests that those molecules strongly impact on immune signaling cascades and cellular immunity. In the present work, LC(50) in six-day-old bees were determined for the following acaricides: tau-fluvalinate, flumethrin, amitraz and coumaphos. According to this obtained value, a group of individuals was treated with each acaricide and then processed for qPCR analysis. Transcript levels for genes encoding antimicrobial peptides and immune-related proteins were assessed. Flumethrin increased the expression of hymenoptaecin when comparing treated and control bees. Significant differences were recorded between coumaphos and flumethrin treatments, while the first one reduced the expression of hymenoptaecin and abaecin, the last one up-regulated their expressions. No significant statistically changes were recorded in the expression levels of vitellogenin, lysozyme or glucose dehydrogenase among bees treated with acaricides and control bees. This work constitutes the first report, under laboratory conditions, about induction of immune related genes in response to synthetic miticides.
authors.affiliation: Laboratorio de Artrópodos, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata-CONICET, Mar del Plata, Buenos Aires, Argentina. pmgarrid@mdp.edu.ar
authors.forename: Paula Melisa
authors.initials: PM
authors.lastname: Garrido
authors.forename: Karina
authors.initials: K
authors.lastname: Antúnez
authors.forename: Mariana
authors.initials: M
authors.lastname: Martín
authors.forename: Martín Pablo
authors.initials: MP
authors.lastname: Porrini
authors.forename: Pablo
authors.initials: P
authors.lastname: Zunino
authors.forename: Martín Javier
authors.initials: MJ
authors.lastname: Eguaras
mesh_terms: D056810:Acaricides / Q000138:chemical synthesis / Q000494:pharmacology*; D000818:Animals; D001516:Bees / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development* / Q000276:immunology; D015870:Gene Expression / Q000187:drug effects*; D019476:Insect Proteins / Q000235:genetics* / Q000276:immunology
chemical_list: D056810:Acaricides; D019476:Insect Proteins