title: Systematic reconstruction of RNA functional motifs with high-throughput microfluidics.
abstract: We present RNA-mechanically induced trapping of molecular interactions (RNA-MITOMI), a microfluidic platform that allows integrated synthesis and functional assays for programmable RNA libraries. The interaction of a comprehensive library of RNA mutants with stem-loop-binding protein precisely defined the RNA structural and sequence features that govern affinity. The functional motif reconstructed in a single experiment on our platform uncovers new binding specificities and enriches interpretation of phylogenetic data.
authors.affiliation: Program in Epithelial Biology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Lance
authors.initials: L
authors.lastname: Martin
authors.forename: Matthias
authors.initials: M
authors.lastname: Meier
authors.forename: Shawn M
authors.initials: SM
authors.lastname: Lyons
authors.forename: Rene V
authors.initials: RV
authors.lastname: Sit
authors.forename: William F
authors.initials: WF
authors.lastname: Marzluff
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Quake
authors.forename: Howard Y
authors.initials: HY
authors.lastname: Chang
mesh_terms: D000818:Animals; D001483:Base Sequence; D015723:Gene Library; D005982:Glutathione Transferase; D006801:Humans; D044085:Microfluidics / Q000379:methods*; D059372:Nucleotide Motifs; D010802:Phylogeny; D012313:RNA / Q000737:chemistry*; D015347:RNA Probes
chemical_list: D015347:RNA Probes; D012313:RNA; D005982:Glutathione Transferase