title: Staphylococcus aureus infection of intestinal epithelial cells induces human umbilical cord-derived mesenchymal stem cell migration.
abstract: There is a growing interest in umbilical cord blood (UCB)-derived mesenchymal stem cells (MSCs) for cellular therapy in regenerative medicine. To aid in tissue repair, MSCs are recruited to sites of inflammation induced by a bacterial infection. The primary objective of this study was to explore the mechanisms of MSC recruitment to intestinal epithelial cells infected with Staphylococcus aureus. First, we isolated and characterized the UCB-derived MSCs used in our experiments. Next, we determined the ability of S. aureus infected intestinal epithelial cells to induce migration of UCB-derived MSCs. Expression analysis of cytokines secreted by infected epithelial cells indicated that MSC migration occurred predominately via a nuclear factor-kappa B (NF-κB)-dependent signaling pathway. Altogether, our data provide the first evidence for a role of S. aureus infection in MSC migration and reveal the function of UCB-derived MSCs in intestinal pathophysiology.
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Ya-hui
authors.initials: YH
authors.lastname: Liu
authors.forename: Zhi-jie
authors.initials: ZJ
authors.lastname: Li
authors.forename: Ming-yuan
authors.initials: MY
authors.lastname: Liu
authors.forename: Ya-gang
authors.initials: YG
authors.lastname: Li
authors.forename: Hu
authors.initials: H
authors.lastname: Jin
authors.forename: Xue-lin
authors.initials: XL
authors.lastname: Wang
authors.forename: Wen-yu
authors.initials: WY
authors.lastname: Han
authors.forename: Jian
authors.initials: J
authors.lastname: Suo
mesh_terms: D002465:Cell Movement; D016207:Cytokines / Q000276:immunology; D004847:Epithelial Cells / Q000276:immunology* / Q000382:microbiology; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology; D059630:Mesenchymal Stem Cells / Q000276:immunology*; D016328:NF-kappa B / Q000276:immunology; D013203:Staphylococcal Infections / Q000276:immunology*; D013211:Staphylococcus aureus; D014470:Umbilical Cord / Q000166:cytology
chemical_list: D016207:Cytokines; D016328:NF-kappa B