title: ANT-VDAC1 interaction is direct and depends on ANT isoform conformation in vitro.
abstract: The voltage-dependent anion channel (VDAC) and the adenine nucleotide translocase (ANT) have central roles in mitochondrial functions such as nucleotides transport and cell death. The interaction between VDAC, an outer mitochondrial membrane protein and ANT, an inner membrane protein, was studied in isolated mitochondria and in vitro. Both proteins were isolated from various mitochondrial sources and reconstituted in vitro using a biomimetic system composed of recombinant human VDAC isoform 1 (rhVDAC1) immobilized on a surface plasmon resonance (SPR) sensor chip surface. Two enriched-preparations of (H)ANT (ANT from heart, mainly ANT1) and (L)ANT (ANT from liver, mainly ANT2) isoforms interacted differently with rhVDAC1. Moreover, the pharmacological ANT inhibitors atractyloside and bongkrekic acid modulated this interaction. Thus, ANT-VDAC interaction depends both on ANT isoform identity and on the conformation of ANT.
authors.affiliation: UMR 5253 CNRS-ENSCM UM2-UM1, Montpellier, France.
authors.forename: Maya
authors.initials: M
authors.lastname: Allouche
authors.forename: Claire
authors.initials: C
authors.lastname: Pertuiset
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Robert
authors.forename: Cécile
authors.initials: C
authors.lastname: Martel
authors.forename: Rémi
authors.initials: R
authors.lastname: Veneziano
authors.forename: Céline
authors.initials: C
authors.lastname: Henry
authors.forename: Ossama Sharaf el
authors.initials: OS
authors.lastname: dein
authors.forename: Nathalie
authors.initials: N
authors.lastname: Saint
authors.forename: Catherine
authors.initials: C
authors.lastname: Brenner
authors.forename: Joel
authors.initials: J
authors.lastname: Chopineau
mesh_terms: D000818:Animals; D006801:Humans; D055767:Immobilized Proteins / Q000378:metabolism; D047468:Immunoprecipitation; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D000226:Mitochondrial ADP, ATP Translocases / Q000737:chemistry / Q000378:metabolism*; D011487:Protein Conformation; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D020349:Surface Plasmon Resonance; D050995:Voltage-Dependent Anion Channel 1 / Q000378:metabolism*
chemical_list: D055767:Immobilized Proteins; D007527:Isoenzymes; D011994:Recombinant Proteins; D000226:Mitochondrial ADP, ATP Translocases; D050995:Voltage-Dependent Anion Channel 1