title: Luminometric sub-nanoliter droplet-to-droplet array (LUMDA) and its application to drug screening by phase I metabolism enzymes.
abstract: Here we show the fabrication of the Luminometric Sub-nanoliter Droplet-to-droplet Array (LUMDA chip) by inkjet printing. The chip is easy to be implemented and allows for a multiplexed multi-step biochemical assay in sub-nanoliter liquid spots. This concept is here applied to the integral membrane enzyme CYP3A4, i.e. the most relevant enzymatic target for phase I drug metabolism, and to some structurally-related inhibitors.
authors.affiliation: Scuola Superiore di Catania, Via Valdisavoia, 9 95123 Catania, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Arrabito
authors.forename: Clelia
authors.initials: C
authors.lastname: Galati
authors.forename: Sabrina
authors.initials: S
authors.lastname: Castellano
authors.forename: Bruno
authors.initials: B
authors.lastname: Pignataro
mesh_terms: D062028:Bioprinting / Q000379:methods; D051544:Cytochrome P-450 CYP3A / Q000737:chemistry* / Q000378:metabolism; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D046228:Microarray Analysis / Q000379:methods*; D036103:Nanotechnology / Q000379:methods
chemical_list: D051544:Cytochrome P-450 CYP3A; C510163:CYP3A4 protein, human