title: Comparison of different treatments for oestrous induction in seasonally anovulatory mares.
abstract: The aim of this study was to evaluate the effects of different treatments for induction and synchronization of oestrus and ovulation in seasonally anovulatory mares. Fifteen mares formed the control group (C), while 26 mares were randomly assigned to three treatment groups. Group T1 (n = 11) were treated with oral altrenogest (0.044 mg/kg; Regumate(®) ) during 11 days. Group T2 (n = 7) was intravaginally treated with 1.38 g of progesterone (CIDR(®) ) for 11 days. In group T3 (n = 8), mares were also treated with CIDR(®) , but only for 8 days. All mares received PGF2α 1 day after finishing the treatment. Sonographic evaluation of follicles, pre-ovulatory follicle size and ovulation time was recorded. Progesterone and leptin levels were analysed. Results show that pre-ovulatory follicles were developed after the treatment in 88.5% of mares. However, the pre-ovulatory follicle growth was dispersal, and sometimes it was detected when treatment was not finished. While in mares treated with intravaginal device, the follicle was soon detected (1.5 ± 1.2 days and 2.3 ± 2.0 days in T2 and T3 groups, respectively), in T1 group, the pre-ovulatory follicle was detected slightly later (3.9 ± 1.6 days). The interval from the end of treatment to ovulation did not show significant differences between groups (T1 = 13.1 ± 2.5 days; T2 = 11.0 ± 3.6 days; T3 = 13.8 ± 4.3 days). The pregnancy rate was 47.4%, similar to the rate observed in group C (46.7%; p > 0.05). Initial leptin concentrations were significantly higher in mares, which restart their ovarian activity after treatments, suggesting a role in the reproduction mechanisms in mares. It could be concluded that the used treatments may be effective for oestrous induction in mares during the late phase of the seasonally anovulatory period. Furthermore, they cannot synchronize oestrus, and then, it is necessary to know the reproductive status of mares when these treatments are used for oestrous synchronization.
authors.affiliation: Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Cordoba, Cordoba, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Vizuete
authors.forename: E
authors.initials: E
authors.lastname: Diez
authors.forename: J
authors.initials: J
authors.lastname: Galisteo
authors.forename: E
authors.initials: E
authors.lastname: Agüera
authors.forename: E
authors.initials: E
authors.lastname: Aguilera-Tejero
authors.forename: C C
authors.initials: CC
authors.lastname: Perez-Marín
mesh_terms: D000282:Administration, Intravaginal; D000818:Animals; D000858:Anovulation; D015237:Dinoprost / Q000008:administration & dosage / Q000494:pharmacology; D004334:Drug Administration Schedule; D004971:Estrus / Q000187:drug effects*; D005260:Female; D006736:Horses / Q000502:physiology*; D010062:Ovulation Induction / Q000379:methods / Q000662:veterinary*; D010120:Oxytocics / Q000008:administration & dosage / Q000494:pharmacology; D011247:Pregnancy; D018873:Pregnancy Rate; D011374:Progesterone / Q000008:administration & dosage / Q000494:pharmacology; D011372:Progestins / Q000008:administration & dosage / Q000494:pharmacology; D012621:Seasons; D014204:Trenbolone Acetate / Q000008:administration & dosage / Q000031:analogs & derivatives
chemical_list: D010120:Oxytocics; D011372:Progestins; C023445:altrenogest; D011374:Progesterone; D015237:Dinoprost; D014204:Trenbolone Acetate