title: Effect of recombinant Lactobacillus casei expressing interleukin-10 in dextran sulfate sodium-induced colitis mice.
abstract: OBJECTIVE
  To study the effect of recombinant Lactobacillus casei (L.casei) expressing interleukin (IL)-10 combined with 5-aminosalicylic acid (5-ASA) in dextran sulfate sodium (DSS)-induced colitis mice.
  
  
  METHODS
  Recombinant L. casei CECT 5276, which can secrete IL-10, was constructed. The length of colon tissue, disease activity index (DAI) and histological score (HS) of the mice were determined to evaluate the modeling and the effectiveness of L. casei. Real-time polymerase chain reaction (PCR), Western blot and ELISA were used to determine the levels of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), peroxisome proliferator-activated receptor (PPAR)-γ, interferon (IFN)-γ, transforming growth factor (TGF)-β and IL-10.
  
  
  RESULTS
  Recombinant L. casei expressing IL-10 combined with 5-ASA was more effective than L. casei with 5-ASA. Among the three different concentrations of the recombinant L. casei, the highest concentration group (2 × 10(9) colony-forming units/mL) had the best effectiveness.
  
  
  CONCLUSIONS
  Recombinant L. casei combined with 5-ASA is effective in the treatment of DSS-induced colitis. The possible mechanism might be the blocking of the excessive activation of NF-κB pathway, thus suppressing the release of inflammation-related factors.
authors.affiliation: Department of Gastroenterology, Huashan Hospital, Fudan University, Shanghai, China.
authors.forename: Zhi Bing
authors.initials: ZB
authors.lastname: Qiu
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Jia Jie
authors.initials: JJ
authors.lastname: Chen
authors.forename: Lan
authors.initials: L
authors.lastname: Rong
authors.forename: Wei Qun
authors.initials: WQ
authors.lastname: Ding
authors.forename: Hai Jing
authors.initials: HJ
authors.lastname: Yang
authors.forename: Liang
authors.initials: L
authors.lastname: Zhong
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology*; D003106:Colon / Q000473:pathology*; D016264:Dextran Sulfate; D005260:Female; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000378:metabolism; D007780:Lacticaseibacillus casei; D019804:Mesalamine / Q000627:therapeutic use; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000097:blood; D047495:PPAR gamma / Q000097:blood; D011994:Recombinant Proteins / Q000096:biosynthesis; D012720:Severity of Illness Index; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016328:NF-kappa B; D047495:PPAR gamma; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D019804:Mesalamine; D007371:Interferon-gamma; D016264:Dextran Sulfate