title: [Regulating effect of Ginkgo biloba extract 50 on hippocampal inflammation-related cytokines in senile rats].
abstract: OBJECTIVE
  To investigate the regulating effect of Ginkgo biloba extract 50 (GBE50) on pre-inflammatory factors interleukin-1 beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha) and anti-inflammatory factors interleukin-4 (IL-4), interleukin-10 (IL-10) of hippocampus in senile rats, in order to explore the protective mechanism of GBE50 on central nervous system of senile animals.
  
  
  METHODS
  SD rats were randomly divided into four groups: the normal group, the model group, the GBE50 group and the EGB761 group. Rats were intraperitoneally injected with 100 mg x kg(-1) D-galactose every day for 42 days to establish the senile rat model. At the 21st day, the GBE50 group and the EGB761 group were orally administered with 60 mg x kg(-1) for 21 days. IL-1beta mRNA and TNF-alpha mRNA expressions were detected by real-time fluorescence quantitative PCR assay, IL-1beta and TNF-alpha protein expressions were detected by immunohistochemistry, IL-4 and IL-10 protein contents were detected by ELISA.
  
  
  RESULTS
  D-galactose caused imbalance between pre-inflammatory factors and anti-inflammatory factors of hippocampus in senile rats, GBE50 and EGB761 reduced IL-1beta mRNA expression (P < 0.05) and TNF-alpha and IL-1beta protein level (P < 0.01) and up-regulated IL-10 protein content (P < 0.01, P < 0.05).
  
  
  CONCLUSIONS
  The mechanism of GBE50 in protecting central nervous system is probably related to its effect in mitigating inflammatory of central nervous system.
authors.affiliation: Department of Physiology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. gaiyinghe2012@163.com
authors.forename: Gaiying
authors.initials: G
authors.lastname: He
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.forename: Lili
authors.initials: L
authors.lastname: Wu
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D020441:Ginkgo biloba; D006624:Hippocampus / Q000187:drug effects* / Q000276:immunology; D053583:Interleukin-1beta / Q000032:analysis*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
chemical_list: D053583:Interleukin-1beta; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha