title: Impact of thiamine deficiency on T-cell dependent and T-cell independent antibody production in lake trout.
abstract: Lake trout Salvelinus namaycush on thiamine-replete and thiamine-depleted diets were evaluated for the effects of thiamine status on in vivo responses to the T-dependent antigen trinitophenol (TNP)-keyhole limpet hemocyanin (TNP-KLH), the T-independent antigen trinitrophenol-lipolysaccaharide (TNP-LPS), or Dulbecco's phosphate-buffered saline (DPBS; negative control fish). Plasma antibody concentrations were evaluated for possible differences in total anti-TNP activity as well as differences in response kinetics. Associations between anti-TNP activity and muscle and liver thiamine concentrations as well as ratios of muscle-to-liver thiamine to anti-TNP activity were also examined. Thiamine-depleted lake trout that were injected with TNP-LPS exhibited significantly more anti-TNP activity than thiamine-replete fish. The depleted fish injected with TNP-LPS also exhibited significantly different response kinetics relative to thiamine-replete lake trout. No differences in activity or kinetics were observed between the thiamine-replete and -depleted fish injected with TNP-KLH or in the DPBS negative controls. Anti-TNP activity in thiamine-depleted lake trout injected with TNP-KLH was positively associated with muscle thiamine pyrophosphate (thiamine diphosphate; TPP) concentration. A negative association was observed between the ratio of muscle-to-liver TPP and T-independent responses. No significant associations between anti-TNP activity and tissue thiamine concentration were observed in the thiamine-replete fish. We demonstrated that thiamine deficiency leads to alterations in both T-dependent and T-independent immune responses in lake trout.
authors.affiliation: U.S. Geological Survey, National Fish Health Research Laboratory, Leetown, WV, USA.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Ottinger
authors.forename: Dale C
authors.initials: DC
authors.lastname: Honeyfield
authors.forename: Christine L
authors.initials: CL
authors.lastname: Densmore
authors.forename: Luke R
authors.initials: LR
authors.lastname: Iwanowicz
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D004032:Diet / Q000662:veterinary; D005393:Fish Diseases / Q000276:immunology*; D006433:Hemocyanins / Q000737:chemistry / Q000276:immunology; D010851:Picrates / Q000737:chemistry / Q000276:immunology; D013601:T-Lymphocytes / Q000502:physiology*; D013831:Thiamine / Q000008:administration & dosage / Q000494:pharmacology*; D013832:Thiamine Deficiency / Q000276:immunology / Q000662:veterinary*; D014337:Trout / Q000276:immunology*
chemical_list: D000906:Antibodies; D010851:Picrates; D006433:Hemocyanins; C005858:picric acid; C032808:keyhole-limpet hemocyanin; D013831:Thiamine