title: Effect of developmental dioxin exposure on methylation and expression of specific imprinted genes in mice.
abstract: 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is an endocrine disruptor affecting the reproductive system in humans. The aim of this study was to evaluate the effects of TCDD administered to pregnant mice at two different doses (2-10 ng/kg/day), on imprinted genes in the male offspring. The degree of methylation and the mRNA expression of Snrpn, Peg3 and Igf2r were analyzed in the sperm, skeletal muscle and liver. TCDD administration (10 ng/kg/day) decreased the sperm count in the male offspring. It did not affect methylation but increased mRNA expression of Snrpn, Peg3, Igf2r and Air ncRNA. In muscle and liver, TCDD (10 ng/kg/day) induced increases in methylation and decreases in mRNA expression of Igf2r. These results show that the robust effects of TCDD on the mRNA expression of Snrpn, Peg3 and Igf2r genes in the sperm and of Igf2r in the muscle and liver are unrelated to changes in methylation in their respective genes.
authors.affiliation: Division of Development and Growth, Department of Paediatrics, University of Geneva Medical School, 1211 Geneva 14, Switzerland. Emmanuel.Somm@unige.ch
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Somm
authors.forename: Christelle
authors.initials: C
authors.lastname: Stouder
authors.forename: Ariane
authors.initials: A
authors.lastname: Paoloni-Giacobino
mesh_terms: D000818:Animals; D019175:DNA Methylation; D052244:Endocrine Disruptors / Q000633:toxicity*; D005260:Female; D018392:Genomic Imprinting; D051741:Kruppel-Like Transcription Factors / Q000235:genetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008431:Maternal-Fetal Exchange; D051379:Mice; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D017527:Receptor, IGF Type 2 / Q000235:genetics; D013076:Sperm Count; D055517:snRNP Core Proteins / Q000235:genetics
chemical_list: D052244:Endocrine Disruptors; D051741:Kruppel-Like Transcription Factors; C097901:Peg3 protein, mouse; D000072317:Polychlorinated Dibenzodioxins; D012333:RNA, Messenger; D017527:Receptor, IGF Type 2; D055517:snRNP Core Proteins