title: Preparative separation and purification of sulforaphene from radish seeds by high-speed countercurrent chromatography.
abstract: Sulforaphene, a kind of isothiocyanates, derived from glucoraphenin which is the important ingredient of radish (Raphanus sativus L.) seeds, has shown significant pharmacological activities. In this paper, the separation and purification of sulforaphene from radish seeds, was achieved by high-speed countercurrent chromatography (HSCCC). A two-phase solvent system consisted of n-hexane-ethyl acetate-methanol-water (35:100:35:100, v/v/v/v) was applied. The revolution speed of the separation column, flow rate of the mobile phase and separation temperature were 800 rpm, 2 ml/min and 30°C, respectively. From about 1000 mg amount of the crude plant extract, 249.4 mg of pure sulforaphene was obtained by one-step separation on a 280 ml HSCCC column. The purified sulforaphene was at a high purity of 96.9% and the mass recovery was more than 95%. The purity of sulforaphene was determined by HPLC analysis and its chemical structure was assessed by MS, (1)H NMR, (13)C NMR and DEPT-135 NMR.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, PR China.
authors.forename: Pengqun
authors.initials: P
authors.lastname: Kuang
authors.forename: Dan
authors.initials: D
authors.lastname: Song
authors.forename: Qipeng
authors.initials: Q
authors.lastname: Yuan
authors.forename: Xinhua
authors.initials: X
authors.lastname: Lv
authors.forename: Di
authors.initials: D
authors.lastname: Zhao
authors.forename: Hao
authors.initials: H
authors.lastname: Liang
mesh_terms: D003377:Countercurrent Distribution / Q000379:methods*; D017879:Isothiocyanates / Q000302:isolation & purification*; D010936:Plant Extracts / Q000302:isolation & purification*; D031224:Raphanus / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*
chemical_list: D017879:Isothiocyanates; D010936:Plant Extracts; C473643:sulphoraphene