title: Modification of response behavior of zinc sensing HydHG two-component system using a self-activation loop and genomic integration.
abstract: Characterizing the dynamics of HydHG-a two-component transcriptional regulatory network for exogenous zinc in E. coli-is essential in understanding the biology of these regulatory and signaling pathways. Here, we used a synthetic biology strategy to modify the dynamic characteristics of the HydHG network in two ways. First, a self-activation loop for HydHG network was created under the control of zraP promoter, after which the threshold Zn(2+) concentration for the self-activated HydHG network significantly decreased from 200 to 10 μM. Second, the self-activation loop was integrated into the E. coli genome allowing the threshold Zn(2+) concentration to be elevated to 500 μM. As the threshold Zn(2+) concentration could be modified in both directions, the introduction of a self-activation loop and the entire genomic integration strategy may prove useful for the creation of a two-component bacterial biosensor with varying sensitivities.
authors.affiliation: School of Chemical Engineering and Bioengineering, University of Ulsan, 93 Daehakro, Nam-gu, Ulsan 680-749, Republic of Korea.
authors.forename: Van Dung
authors.initials: VD
authors.lastname: Pham
authors.forename: Sambandam
authors.initials: S
authors.lastname: Ravikumar
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Lee
authors.forename: Soon Ho
authors.initials: SH
authors.lastname: Hong
authors.forename: Ik-Keun
authors.initials: IK
authors.lastname: Yoo
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D016680:Genome, Bacterial; D011401:Promoter Regions, Genetic; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; D015534:Trans-Activators; C114106:zraP protein, E coli; C423908:zraR protein, E coli; C423907:zraS protein, E coli