title: Proteomic profiles of soluble proteins from the esophageal gland in female Meloidogyne incognita.
abstract: Meloidogyne incognita can infect multiple plant species. Proteins synthesized in the esophageal glands and secreted through the stylet of plant parasitic nematodes play critical roles in the plant-nematode interactions. Female M. incognita live for approximately 15days, embedded in a host plant, but their esophageal gland proteins have not yet been comprehensively analyzed. In this study, a new bacterium-contamination-resistant method for collecting soluble proteins from esophageal gland cells (SPEGC) of female M. incognita was established. Approximately 5μg of freeze-dried proteins could be extracted from 150 female M. incognita. Bands of a one-dimensional SDS-polyacrylamide gel were excised after electrophoresis of 20μg of protein and were analyzed. Two hundred and forty-six proteins from SPEGC of female M. incognita were identified by LC-MS/MS. Gene Ontology analysis suggests that many of the secreted proteins are involved in protein or carbohydrate metabolism and proteolysis. Some of the SPEGC (46.3%) were predicted to be secreted through classical or non-classical secretory pathways. The described method presents a new approach for the identification of proteins stored in SPEGC of an important plant parasitic nematode. This global proteomic profile of SPEGC provides a basis for future studies to elucidate the functions of proteins secreted from female M. incognita on plant responses.
authors.affiliation: Laboratory of Plant Nematology, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, China.
authors.forename: Xin-Rong
authors.initials: XR
authors.lastname: Wang
authors.forename: Yovany A
authors.initials: YA
authors.lastname: Moreno
authors.forename: Han-Rong
authors.initials: HR
authors.lastname: Wu
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Li
authors.forename: Jin-Ai
authors.initials: JA
authors.lastname: Zhang
authors.forename: Chong
authors.initials: C
authors.lastname: Yang
authors.forename: Si
authors.initials: S
authors.lastname: Sun
authors.forename: Wei-Jie
authors.initials: WJ
authors.lastname: Ma
authors.forename: Timothy G
authors.initials: TG
authors.lastname: Geary
mesh_terms: D000818:Animals; D004947:Esophagus / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D059467:Transcriptome; D014431:Tylenchoidea / Q000033:anatomy & histology / Q000378:metabolism*
chemical_list: D015801:Helminth Proteins