title: Prevalence and molecular characterization of Cryptosporidium in ostriches (Struthio camelus) on a farm in central Vietnam.
abstract: This study was performed to determine the prevalence and molecular characterization of Cryptosporidium in ostriches on a farm in Khanh Hoa province, central Vietnam. A total of 464 ostrich fecal samples were examined Cryptosporidium oocysts using the modified Ziehl-Neelsen staining method, and 110 (overall prevalence 23.7%) were identified as positive by microscopy. Prevalence of Cryptosporidium in animals of <45 days, 45-60 days, 61-90 days, 91 days-12 months and >12 months was 23.5% (16/68), 33.3% (22/66), 35.2% (68/193), 0 and 5.8% (4/69), respectively (p<0.05). The majority of positive samples scored as the 3+ level of intensity of infection were from 61 to 90 days ostriches. Molecular analysis in the 18S ribosomal RNA, 70 kDa heat shock protein and actin genes demonstrated the presence of only Cryptosporidium avian genotype II in ostriches in central Vietnam.
authors.affiliation: Laboratory of Sustainable Environmental Biology, Graduate School of Agricultural Science, Tohoku University, Naruko-Onsen, Osaki, Miyagi 989-6711, Japan.
authors.forename: Sam Thi
authors.initials: ST
authors.lastname: Nguyen
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Fukuda
authors.forename: Chika
authors.initials: C
authors.lastname: Tada
authors.forename: Vu Vy
authors.initials: VV
authors.lastname: Huynh
authors.forename: Duc Tan
authors.initials: DT
authors.lastname: Nguyen
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nakai
mesh_terms: D000199:Actins / Q000235:genetics; D000367:Age Factors; D000818:Animals; D000829:Animals, Domestic; D001715:Bird Diseases / Q000453:epidemiology* / Q000469:parasitology*; D003430:Cross-Sectional Studies; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D005243:Feces / Q000469:parasitology; D005838:Genotype; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D015995:Prevalence; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D020310:Struthioniformes / Q000469:parasitology*; D014744:Vietnam / Q000453:epidemiology
chemical_list: D000199:Actins; D016054:DNA, Protozoan; D018840:HSP70 Heat-Shock Proteins; D012337:RNA, Ribosomal, 18S