title: Laminin-5γ2 chain expression is associated with tumor cell invasiveness and prognosis of lung squamous cell carcinoma.
abstract: Our previous study showed that tumor budding is a significant indicator of a poor prognosis in lung squamous cell carcinoma patients. Tumor budding-positive (Bud(+)) cases of lung squamous cell carcinoma (SqCC) showed locally aggressive growth, and the positivity was a useful indicator of the lymph node status and prognosis. The present study focused on the clinicopathologic significance of laminin-5γ2 chain expression for local aggressiveness in lung SqCC. Laminin-5γ2 chain immunohistochemical stains in tissue samples were divided into three distinct types: basement membrane (B type; laminin-5γ2 present in basement membrane), cytoplasmic (C type; laminin- 5γ2 present in intracellular matrix), and invasive front (F type; laminin-5γ2 present in cytoplasm, and strongly in part of peripheral nest). The F type was more common in Bud(+) cases than tumor budding-negative (Bud(-)) cases; B and C types were less common in Bud(+) cases (P 〈 0.001). The F type was more closely associated with decreased overall survival than the B and C types (P 〈 0.001 for both). Univariate analysis showed that the F type could be used to predict tumor size, lymph node metastasis, lymphatic invasion, tumor infiltrative patterns, tumor budding, and laminin-5γ2 chain staining. Multivariate analysis showed that laminin-5γ2 chain staining and tumor budding could be used to predict patient mortality (P 〈 0.001 and P = 0.005, respectively). The overall survival rate after curative resection was lower in patients with the F/Bud(+) type than in those with B+C/Bud(-) and B+C/Bud(+) types (P < 0.001 for both, log-rank test), and also lower with the F/Bud(-) type than the B+C/Bud(-) type. On the other hand, there was no significant difference between the F/Bud(+) and F/Bud(-) types. In conclusion, both laminin- 5γ2 chain staining and tumor budding are associated with tumor cell invasiveness and are independent predictors of mortality in lung SqCC patients.
authors.affiliation: Department of General Thoracic Surgery Tokai University School of Medicine, Isehara, Kanagawa 259-1193, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Masuda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kijima
authors.forename: Naoko
authors.initials: N
authors.lastname: Imamura
authors.forename: Naohiro
authors.initials: N
authors.lastname: Aruga
authors.forename: Kenei
authors.initials: K
authors.lastname: Nakazato
authors.forename: Kana
authors.initials: K
authors.lastname: Oiwa
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nakano
authors.forename: Hajime
authors.initials: H
authors.lastname: Watanabe
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ikoma
authors.forename: Makiko
authors.initials: M
authors.lastname: Tanaka
authors.forename: Sadaki
authors.initials: S
authors.lastname: Inokuchi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Iwazaki
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000401:mortality* / Q000473:pathology; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007797:Laminin / Q000096:biosynthesis*; D008175:Lung Neoplasms / Q000378:metabolism* / Q000401:mortality* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D015996:Survival Rate
chemical_list: C400567:LAMC2 protein, human; D007797:Laminin; D009363:Neoplasm Proteins