title: Effect of weight loss, independent of change in diet composition, on apolipoprotein AI kinetic in men with metabolic syndrome.
abstract: We investigated the effect of weight loss, independent of change in diet composition, on HDL and apoAI metabolism in men with metabolic syndrome (MetS). Subjects (19 men with MetS [NCEP-ATPIII]) were fed an isoenergetic Mediterranean-style diet for 5 weeks (all foods provided). Participants then underwent a 20-week free-living period during which they were counseled to restrict energy intake, after which they were again fed an isoenergetic Mediterranean-style diet for 5 weeks. At the end of the two controlled diets, participants received a single bolus of [5,5,5-(2)H(3)] (L)-leucine, and fasting blood samples were collected over a 96 h period. ApoAI kinetic was assessed using multicompartmental modeling of the tracer enrichment data. Participants achieved a 9.1 ± 2.8% reduction in body weight (P < 0.001). Weight loss resulted in an increase in plasma HDL-cholesterol (HDL-C) concentrations of 6.0% (P = 0.059) and HDL(3)-C of 7.9% (P = 0.045), attributable to a reduction in apoAI fractional catabolic rate (-7.8%; P = 0.046) with no change in apoAI production rate (2.2%; P = 0.58). These data indicate that weight loss, independent of variation in diet composition, increases plasma HDL primarily by delaying the catabolism of apoAI.
authors.affiliation: Institute of Nutraceuticals and Functional Foods,  Laval University, Quebec,  Canada, G1V 0A6.
authors.forename: Caroline
authors.initials: C
authors.lastname: Richard
authors.forename: Patrick
authors.initials: P
authors.lastname: Couture
authors.forename: Sophie
authors.initials: S
authors.lastname: Desroches
authors.forename: Alice H
authors.initials: AH
authors.lastname: Lichtenstein
authors.forename: Benoît
authors.initials: B
authors.lastname: Lamarche
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016632:Apolipoprotein A-I / Q000097:blood / Q000378:metabolism*; D008076:Cholesterol, HDL / Q000097:blood; D004032:Diet; D006801:Humans; D007700:Kinetics; D008297:Male; D024821:Metabolic Syndrome / Q000097:blood / Q000378:metabolism / Q000503:physiopathology*; D008875:Middle Aged; D015431:Weight Loss; D055815:Young Adult
chemical_list: D016632:Apolipoprotein A-I; D008076:Cholesterol, HDL