title: An immunostimulating water insoluble β-glucan of an edible hybrid mushroom: isolation and characterization.
abstract: An immunostimulating water-insoluble β-glucan isolated from hot alkaline extract of the fruiting bodies of an edible somatic hybrid mushroom of Pleurotus florida and Calocybe indica var. APK2 showed significant macrophage, splenocyte, and thymocyte activations. On the basis of total hydrolysis, methylation analysis, and NMR experiments ((1)H, (13)C, DQF-COSY, TOCSY, NOESY, DEPT-135, and HSQC), the repeating unit of the polysaccharide is established.
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Kousik
authors.initials: K
authors.lastname: Maity
authors.forename: Surajit
authors.initials: S
authors.lastname: Samanta
authors.forename: Sunil K
authors.initials: SK
authors.lastname: Bhanja
authors.forename: Saikat
authors.initials: S
authors.lastname: Maity
authors.forename: Ipsita K
authors.initials: IK
authors.lastname: Sen
authors.forename: Swatilekha
authors.initials: S
authors.lastname: Maiti
authors.forename: Birendra
authors.initials: B
authors.lastname: Behera
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Maiti
authors.forename: Samir R
authors.initials: SR
authors.lastname: Sikdar
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
mesh_terms: D000363:Agaricales / Q000737:chemistry* / Q000235:genetics*; D000818:Animals; D002236:Carbohydrate Conformation; D050260:Carbohydrate Metabolism; D002478:Cells, Cultured; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D017737:Macrophages, Peritoneal / Q000187:drug effects*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D013154:Spleen / Q000166:cytology; D060168:Thymocytes / Q000166:cytology / Q000187:drug effects; D047071:beta-Glucans / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*
chemical_list: D047071:beta-Glucans