title: Experimental evidence of cell dissemination playing a role in pathogenesis of IgA nephropathy in multiple lymphoid organs.
abstract: BACKGROUND
  Since the pathogenesis of immunoglobulin A (IgA) nephropathy (IgAN) remains unclear, the rationale for current IgAN therapies is still obscure. Recent studies have shown that galactose-deficient IgA1 (GdIgA1) plays a critical role in the pathogenesis of IgAN and can be a non-invasive IgAN biomarker, although the origin of the pathogenic cells producing GdIgA1 is unknown. We examined the cell types and localization of pathogenic cells in IgAN-prone mice.
  
  
  METHODS
  We transplanted bone marrow (BM) or spleen cells with or without specific cell types from IgAN-prone mice, which have many features similar to human IgAN, to identify cell types responsible for the IgAN phenotype and to determine their localization.
  
  
  RESULTS
  BM transplantation and whole spleen cell transfer from IgAN-prone mice reconstituted IgAN in normal and severe combined immunodeficiency mice. Depletion of CD90(+) spleen cells had no affect on reconstitution, whereas CD19(+) B cells from the spleen were sufficient to reconstitute IgAN in both recipients.
  
  
  CONCLUSIONS
  These results indicate that CD19(+) B cells, which can regulate nephritogenic IgA production in a T-cell-independent manner, are responsible for the disease and are disseminated in peripheral lymphoid organs.
authors.affiliation: Division of Nephrology, Department of Internal Medicine, Juntendo University Faculty of Medicine, Tokyo, Japan.
authors.forename: Junichiro
authors.initials: J
authors.lastname: Nakata
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Suzuki
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sato
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Kano
authors.forename: Satoshi
authors.initials: S
authors.lastname: Horikoshi
authors.forename: Jan
authors.initials: J
authors.lastname: Novak
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tomino
mesh_terms: D000818:Animals; D018941:Antigens, CD19 / Q000378:metabolism; D016026:Bone Marrow Transplantation / Q000276:immunology / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D005922:Glomerulonephritis, IGA / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology*; D007070:Immunoglobulin A / Q000378:metabolism; D008221:Lymphoid Tissue / Q000276:immunology / Q000473:pathology* / Q000503:physiopathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D016513:Mice, SCID; D013154:Spleen / Q000276:immunology / Q000473:pathology / Q000637:transplantation; D018800:Thy-1 Antigens / Q000378:metabolism
chemical_list: D018941:Antigens, CD19; D007070:Immunoglobulin A; D018800:Thy-1 Antigens