title: [Changes of heme oxygenase-1 expression in the nigrostriatal system of MPTP-treated SAMP8 mouse].
abstract: OBJECTIVE
  To explore the relationship between the expression of hemeoxygenase-1 (HO-1) and the dopaminergic system impairment in MPTP-treated SAMP8 mice.
  
  
  METHODS
  6-month-old male SAMP8 mice received MPTP (20 mg/kg) subcutaneous injection at 2-h intervals for 4 times,  and the control group was treated with an equal volume of normal  saline. Mice were sacrificed at 6 h,  24 h,  3 d and 8 d after the first injection for the detection of the changes of tyrosine hydroxylase (TH) and HO-1 in the nigrostriatal system by immunohistochemistry and Western blotting.
  
  
  RESULTS
  TH-positive neuronal loss was visible at 6 h (14.23%,  P<0.05),  24 h (23.85%,  P<0.01),  3 d (36.77%,  P<0.001),  and 8 d (45.90%,  P<0.001),  and the significant progression of dopaminergic neuronal loss occurred most prominently in the MPTP group from 24 h to 3 d (24 h vs 3 d,  P<0.05). There was a significant decrease of striatal TH immunoreactive cells  in the MPTP group (P<0.05). Additionally,  HO-1 positive cells were detected in striatum just only at 3 d,  with the increase of HO-1 protein expression in MPTP groups. Western blot analysis showed no change of HO-1 protein levels in the midbrain after MPTP treatment compared to those of the normal  saline group.
  
  
  CONCLUSIONS
  MPTP caused the loss of dopaminergic neuron number and the decrease of TH protein levels in SAMP8 mice. The up-regulation of HO-1 was ephemeral,  and its effects related with Parkinson's disease was limited in this study.
authors.affiliation: Department of Neurology, Hebei Medical University, Tangshan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Xu
authors.initials: X
authors.lastname: Tong
authors.forename: Su-jie
authors.initials: SJ
authors.lastname: Wang
authors.forename: Xian-min
authors.initials: XM
authors.lastname: Lv
authors.forename: Yue-feng
authors.initials: YF
authors.lastname: Zhang
authors.forename: Yan-yong
authors.initials: YY
authors.lastname: Wang
authors.forename: Ming-wei
authors.initials: MW
authors.lastname: Wang
mesh_terms: D000375:Aging; D000818:Animals; D003342:Corpus Striatum / Q000201:enzymology*; D004195:Disease Models, Animal; D051547:Heme Oxygenase-1 / Q000235:genetics*; D020267:MPTP Poisoning / Q000201:enzymology*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D013378:Substantia Nigra / Q000201:enzymology*; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
chemical_list: D008565:Membrane Proteins; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse; D014446:Tyrosine 3-Monooxygenase