title: Proposed short-term model of acute allergic response, without adjuvant use, in the lungs of mice.
abstract: OBJECTIVE
  To determine whether a short-term protocol using subcutaneous sensitization with ovalbumin, without the use of adjuvants, would induce an eosinophilic response in the lungs of mice similar to that observed in previous, well-established protocols.
  
  
  METHODS
  Adult female BALB/c mice were randomized and divided into groups according to the number of sensitizations with ovalbumin and the number/dosage of intranasal ovalbumin challenges. The short-term protocol (10 days) consisted of one sensitization with ovalbumin and three ovalbumin challenges (100 µg). Total and differential cell counts in BAL fluid, levels of eosinophil peroxidase in lung tissue, and histopathological examination of the lungs were performed 24 h after the last ovalbumin challenge.
  
  
  RESULTS
  No significant differences were found among the groups regarding the variables studied. The short-term protocol, as well as the other protocols studied, induced an eosinophilic response similar to that obtained in the positive control.
  
  
  CONCLUSIONS
  Subcutaneous sensitization with ovalbumin and without the use of adjuvants resulted in a significant allergic response in the lungs of mice, even in the short-term protocol group. Our findings suggest that this short-term protocol can be used as a first-line pre-clinical test for the study of new medications, reducing the costs and observation periods.
authors.affiliation: PUCRS, Pontifical Catholic University of Rio Grande do Sul – Porto Alegre, Brazil. andream_rodrigues@hotmail.com
authors.forename: Andrea Mendonça
authors.initials: AM
authors.lastname: Rodrigues
authors.forename: Camila Zanelatto Parreira
authors.initials: CZ
authors.lastname: Schmidt
authors.forename: Lucien Peroni
authors.initials: LP
authors.lastname: Gualdi
authors.forename: Raquel Giacomelli
authors.initials: RG
authors.lastname: Cao
authors.forename: Rodrigo Godinho de
authors.initials: RG
authors.lastname: Souza
authors.forename: Ana Cláudia
authors.initials: AC
authors.lastname: Pereira
authors.forename: Nailê Karine
authors.initials: NK
authors.lastname: Nuñez
authors.forename: Alisson Passos
authors.initials: AP
authors.lastname: Schleich
authors.forename: Paulo Márcio Condessa
authors.initials: PM
authors.lastname: Pitrez
mesh_terms: D000208:Acute Disease; D000818:Animals; D001249:Asthma / Q000201:enzymology / Q000473:pathology*; D016535:Bronchial Hyperreactivity / Q000201:enzymology / Q000473:pathology*; D001985:Bronchial Provocation Tests; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D004195:Disease Models, Animal; D047209:Eosinophil Peroxidase / Q000378:metabolism*; D005260:Female; D008168:Lung / Q000201:enzymology / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin; D011657:Pulmonary Eosinophilia / Q000276:immunology* / Q000473:pathology; D011897:Random Allocation
chemical_list: D010047:Ovalbumin; D047209:Eosinophil Peroxidase