title: Casticin, a flavonoid, potentiates TRAIL-induced apoptosis through modulation             of anti-apoptotic proteins and death receptor 5 in colon cancer cells.
abstract: We investigated the effect of casticin on apoptosis induced by tumor necrosis             factor (TNF)-related apoptosis-inducing ligand (TRAIL). We found that casticin             potentiated TRAIL-induced apoptosis in human colon cancer cells. Casticin downregulated             cell survival proteins including Bcl-xL, Bcl-2, survivin, XIAP and cFLIP, and             induced death receptor 5 (DR5), but had no effect on DR4 and decoy receptors (DcR1             or DcR2). Deletion of DR5 by siRNA significantly reduced the apoptosis induced             by TRAIL and casticin. In addition, casticin induced reactive oxygen species (ROS)             generation in a dose-dependent manner. Collectively, the present study showed             that casticin potentiates TRAIL-induced apoptosis through downregulation of cell             survival proteins and induction of DR5 mediated by ROS.
authors.affiliation: Department of Medical Oncology, the First Affiliated Hospital of South China University, Hengyang, Hunan 421001, PR China. tsy09@126.com
authors.forename: San-Yuan
authors.initials: SY
authors.lastname: Tang
authors.forename: Mei-Zuo
authors.initials: MZ
authors.lastname: Zhong
authors.forename: Guang-Jin
authors.initials: GJ
authors.lastname: Yuan
authors.forename: Su-Ping
authors.initials: SP
authors.lastname: Hou
authors.forename: Lei-Lan
authors.initials: LL
authors.lastname: Yin
authors.forename: Hao
authors.initials: H
authors.lastname: Jiang
authors.forename: Zheng-Υang
authors.initials: ZΥ
authors.lastname: Yu
mesh_terms: D000704:Analysis of Variance; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology*; D058851:GPI-Linked Proteins / Q000378:metabolism; D020868:Gene Silencing; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D053320:Receptors, Tumor Necrosis Factor, Member 10c; D000077022:Survivin; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000494:pharmacology*; D053319:Tumor Necrosis Factor Decoy Receptors / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000378:metabolism; D051020:bcl-X Protein / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; C494811:BCL2L1 protein, human; C107489:BIRC5 protein, human; D005419:Flavonoids; D058851:GPI-Linked Proteins; D051034:Inhibitor of Apoptosis Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D053320:Receptors, Tumor Necrosis Factor, Member 10c; D000077022:Survivin; D053221:TNF-Related Apoptosis-Inducing Ligand; C108661:TNFRSF10C protein, human; C451501:TNFRSF10D protein, human; C505653:TNFSF10 protein, human; D053319:Tumor Necrosis Factor Decoy Receptors; D051636:X-Linked Inhibitor of Apoptosis Protein; C496866:XIAP protein, human; D051020:bcl-X Protein; C054133:casticin