title: Differential gene expression profile associated with the abnormality of bone marrow mesenchymal stem cells in aplastic anemia.
abstract: Aplastic anemia (AA) is generally considered as an immune-mediated bone marrow failure syndrome with defective hematopoietic stem cells (HSCs) and marrow microenvironment. Previous studies have demonstrated the defective HSCs and aberrant T cellular-immunity in AA using a microarray approach. However, little is known about the overall specialty of bone marrow mesenchymal stem cells (BM-MSCs). In the present study, we comprehensively compared the biological features and gene expression profile of BM-MSCs between AA patients and healthy volunteers. In comparison with healthy controls, BM-MSCs from AA patients showed aberrant morphology, decreased proliferation and clonogenic potential and increased apoptosis. BM-MSCs from AA patients were susceptible to be induced to differentiate into adipocytes but more difficult to differentiate into osteoblasts. Consistent with abnormal biological features, a large number of genes implicated in cell cycle, cell division, proliferation, chemotaxis and hematopoietic cell lineage showed markedly decreased expression in BM-MSCs from AA patients. Conversely, more related genes with apoptosis, adipogenesis and immune response showed increased expression in BM-MSCs from AA patients. The gene expression profile of BM-MSCs further confirmed the abnormal biological properties and provided significant evidence for the possible mechanism of the destruction of the bone marrow microenvironment in AA.
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Li
authors.forename: Shaoguang
authors.initials: S
authors.lastname: Yang
authors.forename: Shihong
authors.initials: S
authors.lastname: Lu
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.forename: Jianming
authors.initials: J
authors.lastname: Feng
authors.forename: Wenqian
authors.initials: W
authors.lastname: Li
authors.forename: Fengxia
authors.initials: F
authors.lastname: Ma
authors.forename: Qian
authors.initials: Q
authors.lastname: Ren
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Yizhou
authors.initials: Y
authors.lastname: Zheng
authors.forename: Zhong Chao
authors.initials: ZC
authors.lastname: Han
mesh_terms: D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000378:metabolism / Q000473:pathology; D017209:Apoptosis; D001853:Bone Marrow / Q000473:pathology; D016022:Case-Control Studies; D002454:Cell Differentiation; D049109:Cell Proliferation; D048430:Cell Shape; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000502:physiology; D008875:Middle Aged; D010641:Phenotype; D015398:Signal Transduction; D059467:Transcriptome; D055815:Young Adult