title: The importance of myeloperoxidase enzyme activity in the pathogenesis of Crimean-Congo haemorrhagic fever.
abstract: Crimean-Congo haemorrhagic fever (CCHF) is a disease with a severe course including acute viral haemorrhagic fever, ecchymosis, thrombocytopenia, hepatic function disorder and high mortality. Myeloperoxidase (MPO) is an enzyme located in neutrophil granulocytes and plays an important role in the destruction of phagocytosed micro-organisms. The aim of this study was to analyse MPO enzyme activity in CCHF cases compared with a control group. A total of 47 randomly selected CCHF patients admitted to the Department of Infectious Diseases of Cumhuriyet University Hospital in Sivas, Turkey, were studied, and as a control group, 41 age- and sex-matched individuals without any systemic disease were included in this study. MPO enzyme activity was measured in plasma and leukocytes for both groups by the ELISA method. MPO plasma and MPO leukocyte values were calculated as 57.62 ± 8.85 and 44.84 ± 9.71 in CCHF patients, and 0.79 ± 0.29 and 0.49 ± 0.11 in the controls, respectively. MPO enzyme activity was statistically significantly higher in patients with CCHF when compared to the control group. In conclusion, MPO enzyme activity is directly related to the activation of phagocytic leukocytes, and increases in both the plasma and leukocytes in CCHF patients. The increase of the MPO enzyme activity in leukocytes due to viral load leads to the destruction of the leukocyte. It is thought that MPO enzyme activity in plasma was higher in CCHF patients due to the destruction of leukocytes. MPO enzyme activity may be important in terms of the prognosis in patients with CCHF; however, more extensive studies are required on this subject.
authors.affiliation: Department of Emergency Medicine, Zonguldak State Hospital, Zonguldak, Turkey.
authors.forename: F M K
authors.initials: FMK
authors.lastname: Guven
authors.affiliation: Department of Biochemistry, Zonguldak State Hospital, Zonguldak, Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Aydin
authors.affiliation: Nephrology Clinic, Zonguldak State Hospital, Zonguldak, Turkey.
authors.forename: G
authors.initials: G
authors.lastname: Yildiz
authors.affiliation: Department of Infectious Diseases and Clinical Microbiology, Cumhuriyet University School of Medicine, 58140 Sivas, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Engin
authors.affiliation: Department of Biochemistry, Zonguldak State Hospital, Zonguldak, Turkey.
authors.forename: V K
authors.initials: VK
authors.lastname: Celik
authors.affiliation: Department of Microbiology and Clinical Microbiology, Cumhuriyet University School of Medicine, 58140 Sivas, Turkey.
authors.forename: D
authors.initials: D
authors.lastname: Bakir
authors.affiliation: Department of Biochemistry, Zonguldak State Hospital, Zonguldak, Turkey.
authors.forename: K
authors.initials: K
authors.lastname: Deveci
mesh_terms: D000328:Adult; D000367:Age Factors; D016022:Case-Control Studies; D005260:Female; D003225:Hemorrhagic Fever Virus, Crimean-Congo; D006479:Hemorrhagic Fever, Crimean / Q000175:diagnosis / Q000201:enzymology* / Q000453:epidemiology / Q000821:virology; D006801:Humans; D007962:Leukocytes / Q000201:enzymology / Q000502:physiology; D008297:Male; D008875:Middle Aged; D009195:Peroxidase / Q000097:blood / Q000378:metabolism*; D010587:Phagocytosis / Q000502:physiology; D012737:Sex Factors
chemical_list: D009195:Peroxidase