title: Differential effect of DDT, DDE, and DDD on COX-2 expression in the human trophoblast derived HTR-8/SVneo cells.
abstract: The purpose of this study was to investigate the effect of 1,1,1-trichloro-2,2-bis-(chlorophenyl)ethane (DDT), 1,1-bis-(chlorophenyl)-2,2-dichloroethene (DDE), and 1,1-dichloro-2,2-bis(chlorophenyl)ethane (DDD) isomers on COX-2 expression in a human trophoblast-derived cell line. Cultured HTR-8/SVneo trophoblast cells were exposed to DDT isomers and its metabolites for 24 h, and COX-2 mRNA and protein expression were assessed by RT-PCR, Western blotting, and ELISA. Prostaglandin E₂ production was also measured by ELISA. Both COX-2 mRNA and protein were detected under control (unexposed) conditions in the HTR-8/SVneo cell line. COX-2 protein expression and prostaglandin E₂ production but not COX-2 mRNA levels increased only after DDE and DDD isomers exposure. It is concluded that DDE and DDD exposure induce the expression of COX-2 protein, leading to increased prostaglandin E2 production. Interestingly, the regulation of COX-2 by these organochlorines pesticides appears to be at the translational level.
authors.affiliation: Research Unit in Reproductive Medicine, UMAE en Ginecologia y Obstetricia Luis Castelazo Ayala., IMSS, México D. F., Mexico.
authors.forename: Pablo
authors.initials: P
authors.lastname: Dominguez-Lopez
authors.forename: Laura
authors.initials: L
authors.lastname: Diaz-Cueto
authors.forename: Aleida
authors.initials: A
authors.lastname: Olivares
authors.forename: Alfredo
authors.initials: A
authors.lastname: Ulloa-Aguirre
authors.forename: Fabian
authors.initials: F
authors.lastname: Arechavaleta-Velasco
mesh_terms: D002274:Carcinogens, Environmental / Q000378:metabolism / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D003634:DDT / Q000031:analogs & derivatives / Q000378:metabolism / Q000633:toxicity*; D003633:Dichlorodiphenyl Dichloroethylene / Q000031:analogs & derivatives / Q000378:metabolism / Q000633:toxicity*; D003632:Dichlorodiphenyldichloroethane / Q000031:analogs & derivatives / Q000378:metabolism / Q000633:toxicity*; D015232:Dinoprostone / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D006801:Humans; D007306:Insecticides / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D009994:Osmolar Concentration; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D013237:Stereoisomerism; D014327:Trophoblasts / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism
chemical_list: D002274:Carcinogens, Environmental; D007306:Insecticides; D012333:RNA, Messenger; D003633:Dichlorodiphenyl Dichloroethylene; D003634:DDT; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D015232:Dinoprostone; D003632:Dichlorodiphenyldichloroethane