title: RGG repeats of PrP-like Shadoo protein bind nucleic acids.
abstract: Shadoo (Sho) is a central nervous system glycoprotein with characteristics similar to those of the cellular prion protein PrP(C), each containing a highly conserved hydrophobic domain (HD) and an N-terminal repeat region. Whereas PrP(C) includes histidine-containing octarepeats, the Sho region N-terminal to the HD includes tandem positively charged "RGG boxes", predicted to bind RNA. Here, we demonstrate that Sho binds DNA and RNA in vitro via this arginine-rich region.
authors.affiliation: Centre for Prions and Protein Folding Diseases, University of Alberta, Edmonton, Alberta T6G 2M8, Canada.
authors.forename: Agnes
authors.initials: A
authors.lastname: Lau
authors.forename: Charles E
authors.initials: CE
authors.lastname: Mays
authors.forename: Sacha
authors.initials: S
authors.lastname: Genovesi
authors.forename: David
authors.initials: D
authors.lastname: Westaway
mesh_terms: D000818:Animals; D001120:Arginine / Q000737:chemistry; D058851:GPI-Linked Proteins; D008239:Lysine / Q000737:chemistry; D051379:Mice; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018621:PrPC Proteins / Q000737:chemistry / Q000378:metabolism; D012329:RNA, Bacterial / Q000378:metabolism*; D018911:Trinucleotide Repeats / Q000502:physiology
chemical_list: D058851:GPI-Linked Proteins; D009419:Nerve Tissue Proteins; D018621:PrPC Proteins; D012329:RNA, Bacterial; C523856:Sprn protein, mouse; D001120:Arginine; D008239:Lysine