title: How does the spacer length of cationic gemini lipids influence the lipoplex formation with plasmid DNA? Physicochemical and biochemical characterizations and their relevance in gene therapy.
abstract: Lipoplexes formed by the pEGFP-C3 plasmid DNA (pDNA) and lipid mixtures containing cationic gemini surfactant of the 1,2-bis(hexadecyl dimethyl ammonium) alkanes family referred to as C16CnC16, where n=2, 3, 5, or 12, and the zwitterionic helper lipid, 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine (DOPE) have been studied from a wide variety of physical, chemical, and biological standpoints. The study has been carried out using several experimental methods, such as zeta potential, gel electrophoresis, small-angle X-ray scattering (SAXS), cryo-TEM, gene transfection, cell viability/cytotoxicity, and confocal fluorescence microscopy. As reported recently in a communication (J. Am. Chem. Soc. 2011, 133, 18014), the detailed physicochemical and biological studies confirm that, in the presence of the studied series lipid mixtures, plasmid DNA is compacted with a large number of its associated Na+ counterions. This in turn yields a much lower effective negative charge, qpDNA−, a value that has been experimentally obtained for each mixed lipid mixture. Consequently, the cationic lipid (CL) complexes prepared with pDNA and CL/DOPE mixtures to be used in gene transfection require significantly less amount of CL than the one estimated assuming a value of qDNA−=−2. This drives to a considerably lower cytotoxicity of the gene vector. Depending on the CL molar composition, α, of the lipid mixture, and the effective charge ratio of the lipoplex, ρeff, the reported SAXS data indicate the presence of two or three structures in the same lipoplex, one in the DOPE-rich region, other in the CL-rich region, and another one present at any CL composition. Cryo-TEMand SAXS studies with C16CnC16/DOPE-pDNA lipoplexes indicate that pDNA is localized between the mixed lipid bilayers of lamellar structures within a monolayer of ∼2 nm. This is consistent with a highly compacted supercoiled pDNA conformation compared with that of linear DNA. Transfection studies were carried out with HEK293T, HeLa, CHO, U343, and H460 cells. The α and ρeff values for each lipid mixture were optimized on HEK293T cells for transfection, and using these values, the remaining cells were also transfected in absence (-FBS-FBS) and presence (-FBS+FBS) of serum. The transfection efficiency was higher with the CLs of shorter gemini spacers (n=2 or 3). Each formulation expressed GFP on pDNA transfection and confocal fluorescence microscopy corroborated the results. C16C2C16/DOPE mixtures were the most efficient toward transfection among all the lipid mixtures and, in presence of serum, even better than the Lipofectamine2000, a commercial transfecting agent. Each lipid combination was safe and did not show any significant levels of toxicity. Probably, the presence of two coexisting lamellar structures in lipoplexes synergizes the transfection efficiency of the lipid mixtures which are plentiful in the lipoplexes formed by CLs with short spacer (n=2, 3) than those with the long spacer (n=5, 12).
authors.affiliation: Grupo de Química Coloidal y Supramolecular, Departamento de Química Física I, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Muñoz-Úbeda
authors.forename: Santosh K
authors.initials: SK
authors.lastname: Misra
authors.forename: Ana L
authors.initials: AL
authors.lastname: Barrán-Berdón
authors.forename: Sougata
authors.initials: S
authors.lastname: Datta
authors.forename: Clara
authors.initials: C
authors.lastname: Aicart-Ramos
authors.forename: Pablo
authors.initials: P
authors.lastname: Castro-Hartmann
authors.forename: Paturu
authors.initials: P
authors.lastname: Kondaiah
authors.forename: Elena
authors.initials: E
authors.lastname: Junquera
authors.forename: Santanu
authors.initials: S
authors.lastname: Bhattacharya
authors.forename: Emilio
authors.initials: E
authors.lastname: Aicart
mesh_terms: D000818:Animals; D016466:CHO Cells; D002412:Cations / Q000737:chemistry*; D002470:Cell Survival; D055598:Chemical Phenomena; D006224:Cricetinae; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D015316:Genetic Therapy; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D018613:Microscopy, Confocal; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010957:Plasmids / Q000737:chemistry*; D053838:Scattering, Small Angle; D013501:Surface-Active Agents / Q000737:chemistry; D014162:Transfection; D014783:Viscosity; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D002412:Cations; D008055:Lipids; D008081:Liposomes; D010714:Phosphatidylethanolamines; D013501:Surface-Active Agents; D049452:Green Fluorescent Proteins; C094877:dioleoyl phosphatidylethanolamine; D004247:DNA