title: Propolis modulates vitronectin, laminin, and heparan sulfate/heparin expression during experimental burn healing.
abstract: OBJECTIVE
  This study was aimed at assessing the dynamics of vitronectin (VN), laminin (LN), and heparan sulfate/heparin (HS/HP) content changes during experimental burn healing.
  
  
  METHODS
  VN, LN, and HS/HP were isolated and purified from normal and injured skin of domestic pigs, on the 3rd, 5th, 10th, 15th, and 21st days following thermal damage. The wounds were treated with apitherapeutic agent (propolis), silver sulfadiazine (SSD), physiological salt solution, and propolis vehicle. VN and LN were quantified using an immunoenzymatic assay and HS/HP was estimated by densitometric analysis.
  
  
  RESULTS
  Propolis treatment stimulated significant increases in VN, LN, and HS/HP contents during the initial phase of study, followed by a reduction in the estimated extracellular matrix molecules. Similar patterns, although less extreme, were observed after treatment with SSD.
  
  
  CONCLUSIONS
  The beneficial effects of propolis on experimental wounds make it a potential apitherapeutic agent in topical burn management.
authors.affiliation: Department of Clinical Chemistry and Laboratory Diagnostics, Medical University of Silesia, 41-200 Sosnowiec, Poland. polczyk@sum.edu.pl
authors.forename: Paweł
authors.initials: P
authors.lastname: Olczyk
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Komosińska-Vassev
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Winsz-Szczotka
authors.forename: Ewa M
authors.initials: EM
authors.lastname: Koźma
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Wisowski
authors.forename: Jerzy
authors.initials: J
authors.lastname: Stojko
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Klimek
authors.forename: Krystyna
authors.initials: K
authors.lastname: Olczyk
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D002056:Burns / Q000188:drug therapy* / Q000378:metabolism; D006493:Heparin / Q000096:biosynthesis* / Q000378:metabolism; D007797:Laminin / Q000096:biosynthesis* / Q000378:metabolism; D011429:Propolis / Q000494:pharmacology*; D012837:Silver Sulfadiazine / Q000494:pharmacology; D013552:Swine; D019096:Vitronectin / Q000096:biosynthesis* / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D000891:Anti-Infective Agents, Local; D007797:Laminin; D019096:Vitronectin; D006493:Heparin; D011429:Propolis; D012837:Silver Sulfadiazine