title: An SSR-based genetic map of pepper (Capsicum annuum L.) serves as an anchor for the alignment of major pepper maps.
abstract: Of the Capsicum peppers (Capsicum spp.), cultivated C. annuum is the most commercially important, but has lacked an intraspecific linkage map based on sequence-specific PCR markers in accord with haploid chromosome numbers. We constructed a linkage map of pepper using a doubled haploid (DH) population derived from a cross between two C. annuum genotypes, a bell-type cultivar 'California Wonder' and a Malaysian small-fruited cultivar 'LS2341 (JP187992)', which is used as a source of resistance to bacterial wilt (Ralstonia solanacearum). A set of 253 markers (151 SSRs, 90 AFLPs, 10 CAPSs and 2 sequence-tagged sites) was on the map which we constructed, spanning 1,336 cM. This is the first SSR-based map to consist of 12 linkage groups, corresponding to the haploid chromosome number in an intraspecific cross of C. annuum. As this map has a lot of PCR-based anchor markers, it is easy to compare it to other pepper genetic maps. Therefore, this map and the newly developed markers will be useful for cultivated C. annuum breeding.
authors.affiliation: Agriculture and Forestry Technology Department, Kyoto Prefectural Agriculture, Forestry and Fisheries Technology Centre , Amarube-cho, Kameoka, Kyoto 621-0806, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Mimura
authors.forename: Takahiro
authors.initials: T
authors.lastname: Inoue
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Minamiyama
authors.forename: Nakao
authors.initials: N
authors.lastname: Kubo
keywords: 12 linkage groups; SSR markers; genetic map; pepper (Capsicum annuum L.)