title: Activation of the Ahr signalling pathway by polychlorobiphenyls causes a marked induction of cytochrome P450 only after depletion of vitellogenin in Sparus aurata.
abstract: The effects of polychlorinated biphenyl congeners 126 and 153 (PCB-126 and PCB-153) on vitellogenin (Vtg) and cytochrome (CYP1A1) expression were evaluated in 60 juvenile Sparus aurata. Fish were divided into four groups and the control group (Group A) was compared to fish exposed to PCB-126 (10-8M) (Group B), PCB-153 (10-6M) (Group C) singly and also in combination (Group D) for 12, 24 and 72 h. Hepatic expression of Vtg and CYP1A1 were analyzed using histological examinations and by immunochemical (Western blotting and immunohistochemistry) methods. Vtg increased in juvenile fishes of Groups B, C and D after 12h respect to Group A and decreased after 24 and 72 h respect to 12h in each group. CYP1A1 increased after 12 and 24h in all groups vs control group and increased in Group B only at 72 h vs in control group. The results showed that chemical interaction and endocrine disruption in fish might produce deleterious consequences not only for fish but also for human.
authors.affiliation: Department of Experimental Sciences and Applied Biotechnology, Faculty of Veterinary Medicine, University of Messina, Polo Universitario Annunziata, 90128 Messina, Italy.
authors.forename: Margherita
authors.initials: M
authors.lastname: Calò
authors.forename: Stefania
authors.initials: S
authors.lastname: Casella
authors.forename: Michele
authors.initials: M
authors.lastname: Panzera
authors.forename: Patrizia
authors.initials: P
authors.lastname: Lo Cascio
authors.forename: Daniela
authors.initials: D
authors.lastname: Alberghina
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Piccione
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D052244:Endocrine Disruptors / Q000633:toxicity; D004790:Enzyme Induction / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism*; D021541:Sea Bream; D014819:Vitellogenins / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D052244:Endocrine Disruptors; D018336:Receptors, Aryl Hydrocarbon; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls; D019363:Cytochrome P-450 CYP1A1; C023035:3,4,5,3',4'-pentachlorobiphenyl; C014024:2,4,5,2',4',5'-hexachlorobiphenyl