title: Preparation of an adipogenic hydrogel from subcutaneous adipose tissue.
abstract: The ability to generate controlled amounts of adipose tissue would greatly ease the burden on hospitals for reconstructive surgery. We have previously shown that a tissue engineering chamber containing a vascular pedicle was capable of forming new fat; however, further refinements are required to enhance fat formation. The development and maintenance of engineered adipose tissue requires a suitable source of growth factors and a suitable scaffold. A hydrogel derived from adipose tissue may fulfil this need. Subcutaneous fat was processed into a thermosensitive hydrogel we refer to as adipose-derived matrix (ADM). Protein analysis revealed high levels of basement membrane proteins, collagens and detectable levels of growth factors. Adipose-derived stem cells exposed to this hydrogel differentiated into adipocytes with >90% efficiency and in vivo testing in rats showed significant signs of adipogenesis after 8 weeks. ADM's adipogenic properties combined with its simple gelation, relatively long shelf life and its tolerance to multiple freeze-thaw cycles, makes it a promising candidate for adipose engineering applications.
authors.affiliation: O'Brien Institute, Fitzroy, Victoria, Australia. christopher.poon@alumni.unimelb.edu.au
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Poon
authors.forename: Maria V
authors.initials: MV
authors.lastname: Pereira E Cotta
authors.forename: Shiba
authors.initials: S
authors.lastname: Sinha
authors.forename: Jason A
authors.initials: JA
authors.lastname: Palmer
authors.forename: Alan A
authors.initials: AA
authors.lastname: Woods
authors.forename: Wayne A
authors.initials: WA
authors.lastname: Morrison
authors.forename: Keren M
authors.initials: KM
authors.lastname: Abberton
mesh_terms: D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D050151:Subcutaneous Fat / Q000737:chemistry*; D034421:Sus scrofa
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate