title: Spatial control of DNA reaction networks by DNA sequence.
abstract: We have developed a set of DNA circuits that execute during gel electrophoresis to yield immobile, fluorescent features in the gel. The parallel execution of orthogonal circuits led to the simultaneous production of different fluorescent lines at different positions in the gel. The positions of the lines could be rationally manipulated by changing the mobilities of the reactants. The ability to program at the nanoscale so as to produce patterns at the macroscale is a step towards programmable, synthetic chemical systems for generating defined spatiotemporal patterns.
authors.affiliation: Institute of Cell and Molecular Biology, University of Texas at Austin, 1 University Station A4800, Austin, TX 78712-0159, USA.
authors.forename: Peter B
authors.initials: PB
authors.lastname: Allen
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Ellington
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D020029:Base Pairing; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D024202:Electrophoretic Mobility Shift Assay; D005456:Fluorescent Dyes / Q000737:chemistry; D013816:Thermodynamics
chemical_list: D000180:Acrylic Resins; D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; C016680:polyacrylamide gels; D004247:DNA