title: Rapid simultaneous identification and determination of the multiple compounds in crude Fructus Corni and its processed products by HPLC-MS/MS with multiple reaction monitoring mode.
abstract: BACKGROUND
  Fructus Corni, a traditional Chinese medicines, is derived from the dry ripe sarcocarp of Cornus officinalis Sieb. et Zucc (Cornaceae). Gallic acid, 5-hydroxymethylfurfural, morroniside, sweroside, loganin, cornin, 7-O-methyl-morroniside and cornuside are the active constituents of Fructus Corni used in many traditional Chinese medicines. This paper describes a sensitive and specific assay for the determination of the eight bioactive compounds in crude and processed Fructus Corni extracts.
  
  
  METHODS
  In this paper, the eight components were determined by high performance liquid chromatography coupled with triple quadrupole mass spectrometry (MS/MS). Quantization was based on multiple reaction monitoring using the precursor production combination for determination of the eight analytes. The analysis was performed on an Agilent Zorbax Extend C(18) column (100 mm × 3.0 mm, 3.5 μm), and an electrospray ionization (ESI)-tandem interface in the positive and negative ion polarity mode was employed prior to mass spectrometric detection.
  
  
  RESULTS
  With the optimized conditions, the eight bioactive compounds were detected properly within 10 min. The developed method showed good precision and reproducibility with the limits of detection ranged from 0.0042 to 12.7875 ng/mL and the average recoveries ranged from 97.08 to 103.7%.
  
  
  CONCLUSIONS
  This newly established method is validated as simple, reliable and accurate. It can be used as a valid analytical method for intrinsic quality control of crude and processed Fructus Corni.
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Cai
authors.forename: Gang
authors.initials: G
authors.lastname: Cao
authors.forename: Baochang
authors.initials: B
authors.lastname: Cai
mesh_terms: D002851:Chromatography, High Pressure Liquid; D029768:Cornus / Q000737:chemistry* / Q000254:growth & development; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D020834:Ethnopharmacology; D005638:Fruit / Q000737:chemistry* / Q000254:growth & development; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D005707:Gallic Acid / Q000032:analysis* / Q000737:chemistry; D057889:Iridoid Glycosides / Q000032:analysis* / Q000737:chemistry; D007536:Isomerism; D057230:Limit of Detection; D008745:Methylation; D011786:Quality Control; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D004365:Drugs, Chinese Herbal; D057889:Iridoid Glycosides; D005707:Gallic Acid; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde