title: Novel in Vitro Efficiency of Chitosan Biomolecule against Trichomonas gallinae.
abstract: BACKGROUND
  Development of new natural agents for parasitic diseases treatment has unexpectedly increased to overcome effectively against emergence and re-emergence of parasitic diseases, the appearance of drug resistant organisms and toxic side effects of current agents. The aim of the study was to evaluate antiprotozoal activities of chitosan biomolecule on trophozoites of Trichomonas gallinae.
  
  
  METHODS
  The antitrichomonal activity of various low molecular weight chitosan concentrations including 125, 250, 500 and 1250 µg ml(-1) against T. gallinae trophozoites cultured in trypticase-yeast extract-maltose medium supplemented with heat-inactivated cold horse serum was evaluated in vitro. Samples containing medium without chitosan were also assayed as controls.
  
  
  RESULTS
  The mortality rates at 0, 3 and 6 h post treatment with all concentrations were significantly different from control group (P<0.05). Treated trophozoites showed more susceptibility to the highest concentration reaching mortality rate of 100% at 3h post inoculation. However, at this time, results for 125, 250 and 500 µg ml(-1) were 93%, 95% and 96.7%, respectively.
  
  
  CONCLUSIONS
  The results demonstrate that the application of chitosan biomolecule is a promising option for treatment of trichomoniasis in pigeons.
authors.affiliation: Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Tavassoli
authors.forename: A
authors.initials: A
authors.lastname: Imani
authors.forename: H
authors.initials: H
authors.lastname: Tajik
authors.forename: M
authors.initials: M
authors.lastname: Moradi
authors.forename: Sh
authors.initials: Sh
authors.lastname: Pourseyed
keywords: Antiparasitic drugs; Chitosan; In Vitro; Trichomonas gallinae; Trophozoite