title: Coupling the Torpedo microplate-receptor binding assay with mass spectrometry to detect cyclic imine neurotoxins.
abstract: Cyclic imine neurotoxins constitute an emergent family of neurotoxins of dinoflagellate origin that are potent antagonists of nicotinic acetylcholine receptors. We developed a target-directed functional method based on the mechanism of action of competitive agonists/antagonists of nicotinic acetylcholine receptors for the detection of marine cyclic imine neurotoxins. The key step for method development was the immobilization of Torpedo electrocyte membranes rich in nicotinic acetylcholine receptors on the surface of microplate wells and the use of biotinylated-α-bungarotoxin as tracer. Cyclic imine neurotoxins competitively inhibit biotinylated-α-bungarotoxin binding to Torpedo-nicotinic acetylcholine receptors in a concentration-dependent manner. The microplate-receptor binding assay allowed rapid detection of nanomolar concentrations of cyclic imine neurotoxins directly in shellfish samples. Although highly sensitive and specific for the detection of neurotoxins targeting nicotinic acetylcholine receptors as a class, the receptor binding assay cannot identify a given analyte. To address the low selectivity of the microplate-receptor binding assay, the cyclic imine neurotoxins tightly bound to the coated Torpedo nicotinic receptor were eluted with methanol, and the chemical nature of the eluted ligands was identified by mass spectrometry. The immobilization of Torpedo electrocyte membranes on the surface of microplate wells proved to be a high-throughput format for the survey of neurotoxins targeting nicotinic acetylcholine receptors directly in shellfish matrixes with high sensitivity and reproducibility.
authors.affiliation: Centre de Recherche CNRS de Gif-sur-Yvette, Institut Fédératif de Neurobiologie Alfred Fessard FR, Laboratoire de Neurobiologie et Développement UPR, France. araoz@inaf.cnrs-gif.fr
authors.forename: Rómulo
authors.initials: R
authors.lastname: Aráoz
authors.forename: Suzanne
authors.initials: S
authors.lastname: Ramos
authors.forename: Franck
authors.initials: F
authors.lastname: Pelissier
authors.forename: Vincent
authors.initials: V
authors.lastname: Guérineau
authors.forename: Evelyne
authors.initials: E
authors.lastname: Benoit
authors.forename: Natalia
authors.initials: N
authors.lastname: Vilariño
authors.forename: Luis M
authors.initials: LM
authors.lastname: Botana
authors.forename: Armen
authors.initials: A
authors.lastname: Zakarian
authors.forename: Jordi
authors.initials: J
authors.lastname: Molgó
mesh_terms: D000818:Animals; D001681:Biological Assay; D001710:Biotin / Q000737:chemistry; D002038:Bungarotoxins / Q000378:metabolism; D002853:Chromatography, Liquid; D006571:Heterocyclic Compounds / Q000032:analysis* / Q000378:metabolism; D007097:Imines / Q000032:analysis* / Q000378:metabolism; D009498:Neurotoxins / Q000032:analysis* / Q000378:metabolism; D011485:Protein Binding; D011978:Receptors, Nicotinic / Q000378:metabolism*; D012758:Shellfish; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014101:Torpedo / Q000378:metabolism*
chemical_list: D002038:Bungarotoxins; D006571:Heterocyclic Compounds; D007097:Imines; D009498:Neurotoxins; D011978:Receptors, Nicotinic; D001710:Biotin