title: Rapid and sensitive microRNA detection with laminar flow-assisted dendritic amplification on power-free microfluidic chip.
abstract: Detection of microRNAs, small noncoding single-stranded RNAs, is one of the key topics in the new generation of cancer research because cancer in the human body can be detected or even classified by microRNA detection. This report shows rapid and sensitive microRNA detection using a power-free microfluidic device, which is driven by degassed poly(dimethylsiloxane), thus eliminating the need for an external power supply. MicroRNA is detected by sandwich hybridization, and the signal is amplified by laminar flow-assisted dendritic amplification. This method allows us to detect microRNA of specific sequences at a limit of detection of 0.5 pM from a 0.5 µL sample solution with a detection time of 20 min. Together with the advantages of self-reliance of this device, this method might contribute substantially to future point-of-care early-stage cancer diagnosis.
authors.affiliation: Bioengineering Laboratory, RIKEN, Wako, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Arata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Komatsu
authors.forename: Kazuo
authors.initials: K
authors.lastname: Hosokawa
authors.forename: Mizuo
authors.initials: M
authors.lastname: Maeda
mesh_terms: D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics / Q000302:isolation & purification; D002138:Calibration; D015342:DNA Probes / Q000235:genetics; D006801:Humans; D057230:Limit of Detection; D035683:MicroRNAs / Q000235:genetics* / Q000302:isolation & purification; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D025202:Molecular Diagnostic Techniques / Q000295:instrumentation*; D009369:Neoplasms / Q000175:diagnosis; D019095:Point-of-Care Systems; D059629:Signal-To-Noise Ratio
chemical_list: D014408:Biomarkers, Tumor; D015342:DNA Probes; D035683:MicroRNAs