title: Administration of heat shock protein 65 inhibits murine melanoma growth in vivo.
abstract: The association between heat shock protein (HSP) 65 and immune diseases has been investigated for many years. The aim of this study was to explore the antitumor effects and possible antitumor mechanism of HSP65. Mice were immunized with HSP65 via subcutaneous injection. Specific IgG antibodies against HSP65 were detected in the sera of immunized mice by enzyme‑linked immunosorbent assay and verified by western blot analysis. HSP65 effectively inhibited the growth of tumors as well as both the protective and therapeutic antitumor immunities in the melanoma tumor models of mice and prolonged the survival of the tumor-bearing mice. Furthermore, HSP65 also attenuated tumor-induced angiogenesis in the intradermal model and pulmonary metastasis in the tail intravenously injected model of mice. It was demonstrated that the administration of HSP65 is able to effectively inhibit the growth, angiogenesis and metastasis of murine melanoma in vivo and provide new prospects for the immunotherapy of melanoma.
authors.affiliation: Minigene Pharmacy Laboratory, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Xie
authors.forename: Huaqian
authors.initials: H
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Yao
authors.forename: Jing
authors.initials: J
authors.lastname: Hou
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Ma
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yun
authors.initials: Y
authors.lastname: Xing
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.forename: Taiming
authors.initials: T
authors.lastname: Li
authors.forename: Junnian
authors.initials: J
authors.lastname: Zheng
authors.forename: Jingjing
authors.initials: J
authors.lastname: Liu
authors.forename: Rongyue
authors.initials: R
authors.lastname: Cao
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D019496:Cancer Vaccines / Q000008:administration & dosage* / Q000276:immunology; D045744:Cell Line, Tumor; D006360:Heat-Shock Proteins / Q000008:administration & dosage* / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008175:Lung Neoplasms / Q000276:immunology / Q000473:pathology / Q000556:secondary; D008297:Male; D008546:Melanoma, Experimental / Q000473:pathology* / Q000628:therapy*; D051379:Mice; D009389:Neovascularization, Pathologic / Q000276:immunology / Q000628:therapy; D047368:Tumor Burden / Q000276:immunology; D018834:Chaperonin 60; D024101:Mitochondrial Proteins
chemical_list: D000906:Antibodies; D019496:Cancer Vaccines; D006360:Heat-Shock Proteins; D007074:Immunoglobulin G; C557066:Hspd1 protein, mouse; D018834:Chaperonin 60; D024101:Mitochondrial Proteins