title: Determination of Gibberellin A3 residue in fruit samples by liquid chromatography-tandem mass spectrometry.
abstract: A fast, simple, low cost, and high throughput method has been developed for the determination of Gibberellin A3 residue in fruit samples (apple, orange, peach, pear and grape). Analysis is performed by LC-MS/MS operated in the multiple reaction monitoring (MRM) mode, acquiring two specific precursor-product ion transitions per target compound. The method has been validated showing good linearity and selectivity. Limits of quantification (LOQs) were 10μgkg(-1) for apple, orange, peach, pear and grape samples. The average recoveries, measured at three concentration levels (10, 20 and 200μgkg(-1)) were in the range 77.8-96.2% for the compound tested with relative standard deviations below 13.7%. The proposed method is rapid, simple and could be utilised for the routine analysis of Gibberellin A3 in fruit samples.
authors.affiliation: Zhejiang Entry-Exit Inspection and Quarantine Bureau of P.R.C, Hangzhou 310012, China. Electronic address: xw@ziq.gov.cn.
authors.forename: Wen
authors.initials: W
authors.lastname: Xie
authors.forename: Chao
authors.initials: C
authors.lastname: Han
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Zheng
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Qian
authors.forename: Huiying
authors.initials: H
authors.lastname: Ding
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.forename: Chunhua
authors.initials: C
authors.lastname: Lv