title: Pesticide analysis in teas and chamomile by liquid chromatography and gas chromatography tandem mass spectrometry using a modified QuEChERS method: validation and pilot survey in real samples.
abstract: This paper presents the validation of a modified QuEChERS method in four matrices - green tea, red tea, black tea and chamomile. The experiments were carried out using blank samples spiked with a solution of 86 pesticides (insecticides, fungicides and herbicides) at four levels - 10, 25, 50 and 100 μg/kg. The samples were extracted according to the citrate QuEChERS protocol; however, to reduce the amount of coextracted matrix compounds, calcium chloride was employed instead of magnesium sulphate in the clean-up step. The samples were analysed by LC-MS/MS and GC-MS/MS. Included in the scope of validation were: recovery, linearity, matrix effects, limits of detection and quantitation as well as intra-day and inter-day precision. The validated method was used in a real sample survey carried out on 75 samples purchased in ten different countries. In all matrices, recoveries of the majority of compounds were in the 70-120% range and were characterised by precision lower than 20%. In 85% of pesticide/matrix combinations the analytes can be detected quantitatively by the proposed method at the European Union Maximum Residue Level. The analysis of the real samples revealed that large number of teas and chamomiles sold in the European Union contain pesticides whose usage is not approved and also pesticides in concentrations above the EU MRLs.
authors.affiliation: Pesticide Residue Research Group, European Union Reference Laboratory (EURL), Department of Hydrogeology and Analytical Chemistry, University of Almería, Ctra. Sacramento S/N°, La Cañada de San Urbano, 04120 Almería, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Lozano
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Rajski
authors.forename: Noelia
authors.initials: N
authors.lastname: Belmonte-Valles
authors.forename: Ana
authors.initials: A
authors.lastname: Uclés
authors.forename: Samanta
authors.initials: S
authors.lastname: Uclés
authors.forename: Milagros
authors.initials: M
authors.lastname: Mezcua
authors.forename: Amadeo R
authors.initials: AR
authors.lastname: Fernández-Alba
mesh_terms: D020843:Chamomile / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D010573:Pesticide Residues / Q000032:analysis* / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods; D013662:Tea / Q000737:chemistry*
chemical_list: D010573:Pesticide Residues; D013662:Tea