title: Coordinate hyperproduction of SmeZ and SmeJK efflux pumps extends drug resistance in Stenotrophomonas maltophilia.
abstract: A Stenotrophomonas maltophilia mutant that coordinately hyper-expresses three resistance nodulation division-type efflux pump genes, smeZ, smeJ, and smeK, has been identified. SmeZ is responsible for elevating aminoglycoside MICs; SmeJ and SmeK are jointly responsible for elevating tetracycline, minocycline, and ciprofloxacin MICs and conferring levofloxacin resistance. One clinical isolate with this same phenotype was identified from a sample of six, and the isolate also coordinately hyper-expresses smeZ and smeJK, confirming the clinical relevance of our findings.
authors.affiliation: School of Cellular and Molecular Medicine, University of Bristol, Bristol, United Kingdom.
authors.forename: Virginia C
authors.initials: VC
authors.lastname: Gould
authors.forename: Aki
authors.initials: A
authors.lastname: Okazaki
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Avison
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics; D002939:Ciprofloxacin / Q000494:pharmacology / Q000627:therapeutic use; D015870:Gene Expression; D019450:Genes, MDR; D016905:Gram-Negative Bacterial Infections / Q000188:drug therapy* / Q000382:microbiology; D006801:Humans; D064704:Levofloxacin; D026901:Membrane Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008911:Minocycline / Q000494:pharmacology / Q000627:therapeutic use; D015242:Ofloxacin / Q000494:pharmacology / Q000627:therapeutic use; D020615:Stenotrophomonas maltophilia / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D013752:Tetracycline / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D026901:Membrane Transport Proteins; D002939:Ciprofloxacin; D064704:Levofloxacin; D015242:Ofloxacin; D013752:Tetracycline; D008911:Minocycline