title: Stem cells of the lamina propria of human oral mucosa and gingiva develop into mineralized tissues in vivo.
abstract: OBJECTIVE
  To characterize the mineralized tissue formed constitutively in the supracalvarial region of scid mice by a primitive stem cell population (hOMSC) derived from the lamina propria of the human oral mucosa and gingiva.
  
  
  METHODS
  Fibrin-hOMSC constructs were cultured for 14 days at which time point they were analysed for the expression of osteoblastic/cementoblastic markers and implanted between the skin and calvaria bones into scid mice. After 8 weeks, the animals were sacrificed and the implantation sites analysed.
  
  
  RESULTS
  Two-week-old cultures of fibrin-hOMSC constructs expressed osteogenic/cementogenic markers at the gene level. Macroscopic and radiographic examinations revealed mineralized masses at the implantation sites of fibrin-hOMSC constructs. Histology, histochemistry and immunofluorescence showed mineralized masses consisting of avascular cellular and acellular matrices that stained positively for collagen, Ca, cementum attachment protein, cementum protein 1, bone sialoprotein, alkaline phosphatase, osteocalcin, amelogenin and ameloblastin. Positive anti-human nuclear antigen indicated the human origin of the cells. Atomic force microscopy depicted long prismatic structures organized in lamellar aggregates.
  
  
  CONCLUSIONS
  Within the limitation of this study, the results indicate for the first time that fibrin-hOMSC constructs are endowed with the constitutive capacity to develop into mineralized tissues that exhibit certain similarities to cementum and bone.
authors.affiliation: Department of Oral Biology, School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Sandra
authors.initials: S
authors.lastname: Treves-Manusevitz
authors.forename: Lia
authors.initials: L
authors.lastname: Hoz
authors.forename: Heled
authors.initials: H
authors.lastname: Rachima
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Montoya
authors.forename: Ephraim
authors.initials: E
authors.lastname: Tzur
authors.forename: Alexander
authors.initials: A
authors.lastname: Vardimon
authors.forename: A Sampath
authors.initials: AS
authors.lastname: Narayanan
authors.forename: Salomon
authors.initials: S
authors.lastname: Amar
authors.forename: Higinio
authors.initials: H
authors.lastname: Arzate
authors.forename: Sandu
authors.initials: S
authors.lastname: Pitaru
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis; D053523:Amelogenin / Q000096:biosynthesis; D000818:Animals; D001861:Bone Regeneration; D003094:Collagen / Q000096:biosynthesis; D003739:Dental Cementum / Q000378:metabolism / Q000502:physiology*; D005337:Fibrin; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D058574:Integrin-Binding Sialoprotein / Q000096:biosynthesis; D051379:Mice; D016513:Mice, SCID; D009061:Mouth Mucosa / Q000166:cytology*; D015675:Osteocalcin / Q000096:biosynthesis; D017027:Protein Tyrosine Phosphatases / Q000096:biosynthesis; D011506:Proteins / Q000378:metabolism; D012038:Regeneration; D033581:Stem Cell Transplantation; D013234:Stem Cells
chemical_list: D053523:Amelogenin; C510886:CEMP1 protein, human; D058574:Integrin-Binding Sialoprotein; D011506:Proteins; D015675:Osteocalcin; D005337:Fibrin; D003094:Collagen; D000469:Alkaline Phosphatase; C404489:HACD1 protein, human; D017027:Protein Tyrosine Phosphatases