title: Distinct cellular and subcellular distributions of G protein-coupled receptor kinase and arrestin isoforms in the striatum.
abstract: G protein-coupled receptor kinases (GRKs) and arrestins mediate desensitization of G protein-coupled receptors (GPCR). Arrestins also mediate G protein-independent signaling via GPCRs. Since GRK and arrestins demonstrate no strict receptor specificity, their functions in the brain may depend on their cellular complement, expression level, and subcellular targeting. However, cellular expression and subcellular distribution of GRKs and arrestins in the brain is largely unknown. We show that GRK isoforms GRK2 and GRK5 are similarly expressed in direct and indirect pathway neurons in the rat striatum. Arrestin-2 and arrestin-3 are also expressed in neurons of both pathways. Cholinergic interneurons are enriched in GRK2, arrestin-3, and GRK5. Parvalbumin-positive interneurons express more of GRK2 and less of arrestin-2 than medium spiny neurons. The GRK5 subcellular distribution in the human striatal neurons is altered by its phosphorylation: unphosphorylated enzyme preferentially localizes to synaptic membranes, whereas phosphorylated GRK5 is found in plasma membrane and cytosolic fractions. Both GRK isoforms are abundant in the nucleus of human striatal neurons, whereas the proportion of both arrestins in the nucleus was equally low. However, overall higher expression of arrestin-2 yields high enough concentration in the nucleus to mediate nuclear functions. These data suggest cell type- and subcellular compartment-dependent differences in GRK/arrestin-mediated desensitization and signaling.
authors.affiliation: Department of Pharmacology, Vanderbilt University Medical Center, Nashville, Tennessee, USA.
authors.forename: Evgeny
authors.initials: E
authors.lastname: Bychkov
authors.forename: Lilia
authors.initials: L
authors.lastname: Zurkovsky
authors.forename: Mika B
authors.initials: MB
authors.lastname: Garret
authors.forename: Mohamed R
authors.initials: MR
authors.lastname: Ahmed
authors.forename: Eugenia V
authors.initials: EV
authors.lastname: Gurevich
mesh_terms: D000818:Animals; D019393:Arrestin / Q000378:metabolism*; D002467:Cell Nucleus / Q000201:enzymology; D059329:Cholinergic Neurons / Q000166:cytology / Q000201:enzymology; D005260:Female; D054768:G-Protein-Coupled Receptor Kinases / Q000378:metabolism*; D006801:Humans; D007395:Interneurons / Q000166:cytology / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D017072:Neostriatum / Q000166:cytology* / Q000201:enzymology*; D010766:Phosphorylation; D021381:Protein Transport; D051381:Rats; D017207:Rats, Sprague-Dawley; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D019393:Arrestin; D007527:Isoenzymes; D054768:G-Protein-Coupled Receptor Kinases