title: Endoplasmic reticulum export and vesicle formation of the movement protein of Chinese wheat mosaic virus are regulated by two transmembrane domains and depend on the secretory pathway.
abstract: The 37K protein of Chinese wheat mosaic virus (CWMV) belongs to the 30K superfamily of plant virus movement proteins. CWMV 37K trans-complemented the cell-to-cell spread of a movement-defective Potato virus X. CWMV 37K fused to enhanced green fluorescent protein localized to plasmodesmata and formed endoplasmic reticulum (ER)-derived vesicular and large aggregate structures. CWMV 37K has two putative N-terminal transmembrane domains (TMDs). Mutations disrupting TMD1 or TMD2 impaired 37K movement function; those mutants were unable to form ER-derived structures but instead accumulated in the ER. Treatment with Brefeldin A or overexpression of the dominant negative mutant of Sar1 retained 37K in the ER, indicating that ER export of 37K is dependent on the secretory pathway. Moreover, CWMV 37K interacted with pectin methylesterases and mutations in TMD1 or TMD2 impaired this interaction in planta. The results suggest that the two TMDs regulate the movement function and intracellular transport of 37K.
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.
authors.forename: Ida Bagus
authors.initials: IB
authors.lastname: Andika
authors.forename: Shiling
authors.initials: S
authors.lastname: Zheng
authors.forename: Zilong
authors.initials: Z
authors.lastname: Tan
authors.forename: Liying
authors.initials: L
authors.lastname: Sun
authors.forename: Hideki
authors.initials: H
authors.lastname: Kondo
authors.forename: Xueping
authors.initials: X
authors.lastname: Zhou
authors.forename: Jianping
authors.initials: J
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D004721:Endoplasmic Reticulum / Q000378:metabolism* / Q000648:ultrastructure; D015967:Gene Expression Regulation, Viral; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics / Q000378:metabolism*; D053830:Plant Viral Movement Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D031425:Plasmodesmata / Q000378:metabolism; D021381:Protein Transport; D012328:RNA Viruses / Q000235:genetics / Q000378:metabolism; D055571:Secretory Pathway / Q000502:physiology*; D014026:Nicotiana / Q000821:virology; D014908:Triticum / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D053830:Plant Viral Movement Proteins; D014764:Viral Proteins