title: Carotenoid compositions of coloured tomato cultivars and contribution to antioxidant activities and protection against H(2)O(2)-induced cell death in H9c2.
abstract: The carotenoid compositions, antioxidant activities and the potential cardio-protective role of 13 tomato cultivars with distinct colour were studied. Colour coordinates were evaluated by colorimeter and the carotenoid compositions were analysed by UPLC. Red tomatoes had the highest total carotenoid contents (TCC) and antioxidant activities, followed by purple, orange, pink and yellow ones. The TCC were 120.5-278.0 μg/gDW, and the antioxidant activities were 21.32-40.07 μmolTE/gDW (PCL), 64.42-89.98% (DPPH) and 10.47-13.76 μmolTE/g DW (ORAC), respectively. The lipophilic extracts were also found to prevent cell death in a cell-based model system using cardiac H9c2 cells and H(2)O(2), via attenuation of the caspase-3 and matrix metalloproteinase-2 activities. The extracts of different tomatoes showed strong but different antioxidant activities. Roles of total and individual carotenoids in the antioxidant activities were studied and lycopene showed the highest correlation. Results of this study can be used to guide the development of new tomato cultivars and functional foods, and benefit the consumers.
authors.affiliation: State Key Lab of Food Science and Technology, Institute for Advanced Study, Nanchang University, Nanchang 330047, Jiangxi, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.forename: Zeyuan
authors.initials: Z
authors.lastname: Deng
authors.forename: Ronghua
authors.initials: R
authors.lastname: Liu
authors.forename: Steven
authors.initials: S
authors.lastname: Loewen
authors.forename: Rong
authors.initials: R
authors.lastname: Tsao
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D002338:Carotenoids / Q000737:chemistry* / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000633:toxicity*; D018551:Solanum lycopersicum / Q000737:chemistry* / Q000145:classification; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects*; D020011:Protective Agents / Q000737:chemistry* / Q000494:pharmacology; D051381:Rats
chemical_list: D000975:Antioxidants; D020011:Protective Agents; D002338:Carotenoids; D006861:Hydrogen Peroxide