title: Stratum basale keratinocyte expression of the cell-surface glycoprotein CDCP1 during epidermogenesis and its role in keratinocyte migration.
abstract: BACKGROUND
  Epidermogenesis and epidermal wound healing are tightly regulated processes during which keratinocytes must migrate, proliferate and differentiate. Cell-to-cell adhesion is crucial to the initiation and regulation of these processes. CUB-domain-containing protein (CDCP)1 is a transmembrane glycoprotein that is differentially tyrosine phosphorylated during changes in cell adhesion and survival signalling, and is expressed by keratinocytes in native human skin, as well as in primary cultures.
  
  
  OBJECTIVE
  To investigate the expression of CDCP1 during epidermogenesis and its role in keratinocyte migration.
  
  
  METHODS
  We examined both human skin tissue and an in vitro three-dimensional human skin equivalent model to examine the expression of CDCP1 during epidermogenesis. To examine the role of CDCP1 in keratinocyte migration we used a function-blocking anti-CDCP1 antibody and a real-time Transwell™ cell migration assay.
  
  
  RESULTS
  Immunohistochemical analysis indicated that in native human skin CDCP1 is expressed in the stratum basale and stratum spinosum. In contrast, during epidermogenesis in a three-dimensional human skin equivalent model, CDCP1 was expressed only in the stratum basale, with localization restricted to the cell-cell membrane. No expression was detected in basal keratinocytes that were in contact with the basement membrane. Furthermore, an anti-CDCP1 function-blocking antibody was shown to disrupt keratinocyte chemotactic migration in vitro.
  
  
  CONCLUSIONS
  These findings delineate the expression of CDCP1 in human epidermal keratinocytes during epidermogenesis and demonstrate that CDCP1 is involved in keratinocyte migration.
authors.affiliation: Tissue Repair and Regeneration Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, QLD 4059, Australia.
authors.forename: J A
authors.initials: JA
authors.lastname: McGovern
authors.forename: J R
authors.initials: JR
authors.lastname: Heinemann
authors.forename: L J
authors.initials: LJ
authors.lastname: Burke
authors.forename: R
authors.initials: R
authors.lastname: Dawson
authors.forename: T J
authors.initials: TJ
authors.lastname: Parker
authors.forename: Z
authors.initials: Z
authors.lastname: Upton
authors.forename: J D
authors.initials: JD
authors.lastname: Hooper
authors.forename: K J
authors.initials: KJ
authors.lastname: Manton
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000378:metabolism* / Q000502:physiology; D000951:Antigens, Neoplasm; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000378:metabolism* / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D054443:Cell Migration Assays / Q000379:methods; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation; D002633:Chemotaxis / Q000502:physiology; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000378:metabolism*; D008954:Models, Biological; D009363:Neoplasm Proteins / Q000378:metabolism* / Q000502:physiology
chemical_list: D015703:Antigens, CD; D000951:Antigens, Neoplasm; C432044:CDCP1 protein, human; D015815:Cell Adhesion Molecules; D009363:Neoplasm Proteins