title: Molecular characterization of chromosomal band 5p15.33: a recurrent breakpoint region in mantle cell lymphoma involving the TERT-CLPTM1L locus.
abstract: Secondary chromosomal aberrations may contribute to the development of a malignant phenotype in mantle cell lymphoma. Chromosomal band 5p15.33 represents a new recurrent breakpoint in B-cell malignancies. We present a molecular cytogenetic study of 8 mantle cell lymphoma (MCL) cell lines and 23 patients with MCL to determine and characterize novel secondary aberrations. We detected new secondary recurrent rearrangements in all cell lines and in 7 patients and confirmed 5p15.33 as a recurrent breakpoint in 4 cell lines and one patient. Further molecular characterization by flow-FISH and quantitative RT-PCR suggest TERT and CLPTM1L as target genes of 5p15.33 rearrangements.
authors.affiliation: Department of Oncology, Hematology, BMT with Section Pneumology, Hubertus Wald Tumorzentrum - University Cancer Center Hamburg, University Medical Center Hamburg-Eppendorf, Hamburg, Germany. g.schilling@uke.de
authors.forename: Georgia
authors.initials: G
authors.lastname: Schilling
authors.forename: Eva Maria Murga
authors.initials: EM
authors.lastname: Penas
authors.forename: Snjezana
authors.initials: S
authors.lastname: Janjetovic
authors.forename: Leticia
authors.initials: L
authors.lastname: Oliveira-Ferrer
authors.forename: Melanie
authors.initials: M
authors.lastname: Braig
authors.forename: Petra
authors.initials: P
authors.lastname: Behrmann
authors.forename: Carsten
authors.initials: C
authors.lastname: Bokemeyer
authors.forename: Judith
authors.initials: J
authors.lastname: Dierlamm
mesh_terms: D000368:Aged; D045744:Cell Line, Tumor; D002871:Chromosome Banding; D019457:Chromosome Breakage; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics*; D004252:DNA Mutational Analysis; D005260:Female; D005787:Gene Frequency; D056426:Genetic Loci / Q000502:physiology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D020522:Lymphoma, Mantle-Cell / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D015514:Oncogene Proteins, Fusion / Q000235:genetics; D015995:Prevalence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019098:Telomerase / Q000235:genetics*; D014178:Translocation, Genetic / Q000502:physiology
chemical_list: C422445:CLPTM1L protein, human; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D015514:Oncogene Proteins, Fusion; C509186:TERT protein, human; D019098:Telomerase