title: Novel angiotensin I-converting enzyme inhibitory peptides derived from soya milk.
abstract: Inhibitors of angiotensin I-converting enzyme (ACE) are useful in treating hypertension, and many have been derived from food products, including soybeans. Using the industrial protease PROTIN SD-NY10, we developed a processed soya milk (PSM) with enhanced ACE inhibitory activity. The ACE inhibitory activity of PSM (IC(50)=0.26 μg/ml) was greater than that of regular soya milk (IC(50)=8.75 μg/ml). Eight novel ACE inhibitory peptides were purified from PSM by reversed-phase chromatography: FFYY (IC(50),1.9 μM), WHP (4.8 μM), FVP (10.1 μM), LHPGDAQR (10.3 μM), IAV (27.0 μM), VNP (32.5 μM), LEPP (100.1 μM), and WNPR (880.0 μM). The IC(50) values of these peptides are comparable to those reported for other ACE inhibitory peptides derived from wheat, chicken, bonito, wine, etc. Due to the relatively low IC(50) values of several peptides identified in this study, they may serve as ideal base components of food supplements for patients with hypertension.
authors.affiliation: Akita Research Institute of Food and Brewing (ARIF), 4-26 Sanuki, Arayamachi, Akita 010-1623, Japan. tomatsumakoto@pref.akita.lg.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Tomatsu
authors.forename: Atsushi
authors.initials: A
authors.lastname: Shimakage
authors.forename: Mamoru
authors.initials: M
authors.lastname: Shinbo
authors.forename: Seihan
authors.initials: S
authors.lastname: Yamada
authors.forename: Saori
authors.initials: S
authors.lastname: Takahashi
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D007700:Kinetics; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D041942:Soy Milk / Q000737:chemistry*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D010455:Peptides