title: Verification of natural infection of peridomestic rodents by PCV2 on commercial swine farms.
abstract: The porcine circovirus-2 (PCV2) is the main agent responsible for porcine circovirus associated diseases (PCVAD). Few studies have been done regarding PCV2 infection in other species. The purpose of this study was to investigate the occurrence of PCV2 infection in the peridomestic rodent species Mus musculus and Rattus rattus on commercial pig farms in Brazil. Immunohistochemistry assay demonstrated PCV2 in the spleen, lung and kidney. Viral DNA was detected in tissues by nested PCR assay. Partial sequences of PCV2 genomes detected in the rodents had strong identity with gene sequences of PCV2 isolates from pigs. These results show that the studied peridomestic rodent species can be naturally infected by PCV2. However, further studies are needed to confirm PCV2 transmission from rodents to pigs.
authors.affiliation: Laboratório de Infectologia Molecular Animal (LIMA), Departamento de Veterinária, Universidade Federal de Viçosa, Avenida Peter Henry Rolfs s/n, Campus Universitário, 36570-000 Viçosa, Minas Gerais, Brazil.
authors.forename: Albanno Leonard Braz Campos
authors.initials: AL
authors.lastname: Pinheiro
authors.forename: Luiz Henrique Silva
authors.initials: LH
authors.lastname: Bulos
authors.forename: Thiago Souza
authors.initials: TS
authors.lastname: Onofre
authors.forename: Michelle
authors.initials: M
authors.lastname: de Paula Gabardo
authors.forename: Otávio Valério
authors.initials: OV
authors.lastname: de Carvalho
authors.forename: Mariana Costa
authors.initials: MC
authors.lastname: Fausto
authors.forename: Roberto Maurício Carvalho
authors.initials: RM
authors.lastname: Guedes
authors.forename: Márcia Rogéria
authors.initials: MR
authors.lastname: de Almeida
authors.forename: Abelardo
authors.initials: A
authors.lastname: Silva Júnior
mesh_terms: D000822:Animal Husbandry / Q000706:statistics & numerical data; D000818:Animals; D001938:Brazil / Q000453:epidemiology; D018173:Circoviridae Infections / Q000453:epidemiology / Q000635:transmission / Q000662:veterinary*; D017925:Circovirus; D051379:Mice / Q000821:virology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D051381:Rats / Q000821:virology; D012376:Rodent Diseases / Q000453:epidemiology / Q000821:virology*; D013552:Swine / Q000821:virology; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology