title: Timing of transcriptional quiescence during gametogenesis is controlled by global histone H3K4 demethylation.
abstract: Gametes are among the most highly specialized cells produced during development. Although gametogenesis culminates in transcriptional quiescence in plants and animals, regulatory mechanisms controlling this are unknown. Here, we confirm that gamete differentiation in the single-celled yeast Saccharomyces cerevisiae is accompanied by global transcriptional shutoff following the completion of meiosis. We show that Jhd2, a highly conserved JARID1-family histone H3K4 demethylase, activates protein-coding gene transcription in opposition to this programmed transcriptional shutoff, sustaining the period of productive transcription during spore differentiation. Moreover, using genome-wide nucleosome, H3K4me, and transcript mapping experiments, we demonstrate that JHD2 globally represses intergenic noncoding transcription during this period. The widespread transcriptional defects of JHD2 mutants are associated with precocious differentiation and the production of stress-sensitive spores, demonstrating that Jhd2 regulation of the global postmeiotic transcriptional program is critical for the production of healthy meiotic progeny.
authors.affiliation: Department of Molecular Genetics, University of Toronto, Toronto M5S 1A8, Canada.
authors.forename: Mengshu
authors.initials: M
authors.lastname: Xu
authors.forename: Maria
authors.initials: M
authors.lastname: Soloveychik
authors.forename: Mathieu
authors.initials: M
authors.lastname: Ranger
authors.forename: Michael
authors.initials: M
authors.lastname: Schertzberg
authors.forename: Zarna
authors.initials: Z
authors.lastname: Shah
authors.forename: Ryan
authors.initials: R
authors.lastname: Raisner
authors.forename: Shivkumar
authors.initials: S
authors.lastname: Venkatasubrahmanyan
authors.forename: Kyle
authors.initials: K
authors.lastname: Tsui
authors.forename: Marinella
authors.initials: M
authors.lastname: Gebbia
authors.forename: Tim
authors.initials: T
authors.lastname: Hughes
authors.forename: Harm
authors.initials: H
authors.lastname: van Bakel
authors.forename: Corey
authors.initials: C
authors.lastname: Nislow
authors.forename: Hiten D
authors.initials: HD
authors.lastname: Madhani
authors.forename: Marc D
authors.initials: MD
authors.lastname: Meneghini
mesh_terms: D044127:Epigenesis, Genetic; D005718:Gametogenesis / Q000235:genetics* / Q000502:physiology*; D005800:Genes, Fungal; D006657:Histones / Q000378:metabolism*; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics / Q000378:metabolism; D008540:Meiosis; D008745:Methylation; D009154:Mutation; D009707:Nucleosomes / Q000378:metabolism; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D000089804:Shelterin Complex; D013172:Spores, Fungal / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D034501:Telomere-Binding Proteins / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D006657:Histones; D009707:Nucleosomes; C440829:RAP1 protein, S cerevisiae; D012331:RNA, Fungal; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; D000089804:Shelterin Complex; D034501:Telomere-Binding Proteins; D014157:Transcription Factors; C519119:JHD2 protein, S cerevisiae; D056484:Jumonji Domain-Containing Histone Demethylases