title: Gateway vectors for transformation of cereals.
abstract: Until now, the availability of vectors for transgenic research in cereal crops has been rather limited. We present a novel collection of Agrobacterium tumefaciens binary T-DNA vectors compatible with Gateway recombinational cloning that facilitate the modular assembly of genes of interest together with new regulatory sequences, such as strong constitutive or endosperm-specific Brachypodium distachyon promoters. This resource aims at streamlining the creation of vectors and transgenes designed to explore gene functions in vital monocotyledonous crops.
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium.
authors.forename: Mansour
authors.initials: M
authors.lastname: Karimi
authors.forename: Dirk
authors.initials: D
authors.lastname: Inzé
authors.forename: Mieke
authors.initials: M
authors.lastname: Van Lijsebettens
authors.forename: Pierre
authors.initials: P
authors.lastname: Hilson
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics*; D058431:Brachypodium / Q000235:genetics*; D003001:Cloning, Molecular; D018556:Crops, Agricultural / Q000235:genetics; D004269:DNA, Bacterial; D002523:Edible Grain / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D005818:Genetic Engineering; D005822:Genetic Vectors; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D011995:Recombination, Genetic; D014170:Transformation, Genetic; D019076:Transgenes; D003313:Zea mays / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; C036483:T-DNA