title: Rapid detection and identification of viroids in the genus Coleviroid using a universal probe.
abstract: A simple, low-cost hybridization assay using a universal DIG-labeled riboprobe for the rapid detection and identification of coleus viroids is presented. An octamer of 32-nucleotide sequence derived from the central conserved region (CCR) of viroids in the genus Coleviroid was used to develop a universal cRNA probe (8-central-conserved-region probe, 8CCR probe) for coleus viroids. Dot-blot hybridization assays demonstrated that the sensitivity of this probe was similar to specific probes for each CbVd, and Northern hybridization results revealed that at least four coleus viroids could be distinguished readily and simultaneously using the 8CCR probe. Batch detection assay showed that hybridization using the 8CCR probe can identify coleus viroids rapidly and effectively. This rapid and low-cost molecular hybridization technique is an effective way to survey the occurrence of coleus viroids, and has reference for the detection of other viroids and possibly viruses.
authors.affiliation: Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Jiang
authors.forename: Wanying
authors.initials: W
authors.lastname: Hou
authors.forename: Teruo
authors.initials: T
authors.lastname: Sano
authors.forename: Ni
authors.initials: N
authors.lastname: Kang
authors.forename: Lü
authors.initials: L
authors.lastname: Qin
authors.forename: Zujian
authors.initials: Z
authors.lastname: Wu
authors.forename: Shifang
authors.initials: S
authors.lastname: Li
authors.forename: Lianhui
authors.initials: L
authors.lastname: Xie
mesh_terms: D031330:Coleus / Q000821:virology; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014772:Viroids / Q000235:genetics* / Q000302:isolation & purification*; D014773:Virology / Q000191:economics / Q000379:methods*
chemical_list: D015345:Oligonucleotide Probes