title: Dual downregulation of microRNA 17-5p and E2F1 transcriptional factor in pediatric systemic lupus erythematosus patients.
abstract: The main objective of this study is to investigate the relative expression of miRNA 17-5p and one of its target genes E2F1 in the peripheral blood of systemic lupus erythematosus pediatric patients. The expression of miRNA 17-5p and its target E2F1 mRNA was analyzed by TaqMan real-time qPCR. Our results showed significant downregulation of miRNA 17-5p in SLE patients compared to healthy controls; moreover, miRNA 17-5p was more downregulated in patients on no treatment compared to those on treatment. Relative expression of E2F1, which is a target for miRNA 17-5p, was significantly downregulated as well on both mRNA and protein levels in SLE pediatric patients. In conclusion, our data show an unexpected dual downregulation of both miRNA 17-5p and its target gene E2F1 on the mRNA and protein levels. This may suggest an expression pattern of miRNA 17-5p and its target E2F1 that may be specific to SLE. [corrected].
authors.affiliation: The Molecular Pathology Research Group, Department of Pharmacology and Toxicology, Faculty of Pharmacy and Biotechnology, German University in Cairo (GUC New Cairo City), Main Entrance Al Tagamoa Al Khames, Cairo 11835, Egypt. heba.abdelhakam@guc.edu.eg
authors.forename: Heba Ragaee Abdelhakam
authors.initials: HR
authors.lastname: Aboelenein
authors.forename: Samia
authors.initials: S
authors.lastname: Salah
authors.forename: Yasmine Adel
authors.initials: YA
authors.lastname: Lashine
authors.forename: Ahmed Ihab
authors.initials: AI
authors.lastname: Abdelaziz
mesh_terms: D000367:Age Factors; D016022:Case-Control Studies; D002478:Cells, Cultured; D002648:Child; D015536:Down-Regulation; D050687:E2F1 Transcription Factor / Q000097:blood / Q000235:genetics*; D005260:Female; D017930:Genes, Reporter; D005819:Genetic Markers; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D012333:RNA, Messenger / Q000097:blood*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D050687:E2F1 Transcription Factor; C493753:E2F1 protein, human; D005819:Genetic Markers; C524941:MIRN17 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger