title: Effects of different elicitors on 10-deacetylbaccatin III-10-O-acetyltransferase activity and cytochrome P450 monooxygenase content in suspension cultures of Taxus cuspidata cells.
abstract: The effects of four elicitors, including 100 μmol/l MeJA (methyl jasmonate), 40 μl/l hydrogen peroxide (30%, w/w), 80 mg/l SA (salicylic acid) and 0.4 g/l F3 (fungal elicitor), on suspension cultures of Taxus cuspidata were studied. After addition of the above four elicitors, the enzyme activity of 10-DBAT (10-deacetylbaccatin III-10-O-acetyltransferase) was induced and reached its maximum of 5.47, 0.97, 3.30 and 6.82 U, respectively. After elicitation, the concentrations of cytochrome P450 monooxygenase were also induced to its maximum values of 0.069, 0.336, 0.321 and 0.193 nmol/ml, respectively. In addition, under the elicitation, the change in 10-DBAT activity was similar to that of cytochrome P450 monooxygenase concentration. The products of these two enzymes changed after the variety of the enzymes, and the taxol content increased through the cultivation.
authors.affiliation: Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, No.1 QingHuaYuan Street, Haidian District, Beijing, 100084, Peoples Republic of China.
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Zhang
authors.forename: Shan
authors.initials: S
authors.lastname: Gong
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Guo
keywords: 10-DAB III, 10-deacetylbaccatin III; 10-DBAT, 10-deacetylbaccatin III-10-O-acetyltransferase; 10-deacetylbaccatin III; 10-deacetylbaccatin III-10-O-acetyl transferase; 6-BA, 6-benzylaminopurine; DTT, dithiothreitol; DW, dry weight; F3, fungal elicitor; GGPP, geranylgeranyl diphosphate; MeJA, methyl jasmonate; SA, salicylic acid; Taxol; Taxus cuspidata; baccatin III; cytochrome P450 monooxygenase