title: Association between newly identified variant form of DNA polymerase beta (Δ 208-304) and ovarian cancer.
abstract: BACKGROUND
  Base excision repair (BER) is a key pathway for maintaining genomic stability. A key enzyme in the BER pathway is DNA polymerase beta (polβ), which removes the deoxyribose phosphate group (dRP) and fills in the gap with a nucleotide after the DNA lesion is excised. It has been shown that more than thirty percent of breast, bladder, esophageal, colon, and gastric cancer samples studied so far have exhibited DNA polymerase beta mutation.
  
  
  OBJECTIVE
  To examine the association between polβ polymorphism and ovarian cancer, case control study was performed using one hundred fifty two cancer samples and non-metastatic normal samples from the same patients in Indian population.
  
  
  METHODS
  The polβ polymorphism was studied in ovarian carcinoma tissues samples initially by RT-PCR followed by sequencing and then by western blot analysis.
  
  
  RESULTS
  A new type of variant was detected along with the WT allele (polβΔ<formula> _{208-304}</formula>). Stage IV samples have shown a significant factor for cancer progression in ovarian cancer patients of India [OR=3.58; 95% CI (1.6-7.9); and p=0.001]. The association study involving serous type and the variant showed a tendency towards ovarian carcinogenesis [OR=1.57; 95% CI (0.8-3.1); p=0.19]. The western blot analysis result indicates that the specific deletion appears to be associated with disease progression.
  
  
  CONCLUSIONS
  The result reveals that this variant form of polβ is a predisposing factor for stage IV ovarian cancer samples in Indian population.
authors.affiliation: Department of Biotechnology, Haldia Institute of Technology, WB, India.
authors.forename: Kalyani
authors.initials: K
authors.lastname: Khanra
authors.forename: Kakali
authors.initials: K
authors.lastname: Panda
authors.forename: Chandan
authors.initials: C
authors.lastname: Bhattacharya
authors.forename: Ak
authors.initials: A
authors.lastname: Mitra
authors.forename: Ranu
authors.initials: R
authors.lastname: Sarkar
authors.forename: Sipra
authors.initials: S
authors.lastname: Banerjee
authors.forename: Nandan
authors.initials: N
authors.lastname: Bhattacharyya
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D019951:DNA Polymerase beta / Q000235:genetics* / Q000378:metabolism; D004260:DNA Repair; D005260:Female; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000201:enzymology* / Q000235:genetics* / Q000473:pathology; D016019:Survival Analysis; D055815:Young Adult
chemical_list: D019951:DNA Polymerase beta