title: Detection and quantitation of lipid in the microalga Tetraselmis subcordiformis (Wille) Butcher with BODIPY 505/515 staining.
abstract: BODIPY 505/515, a lipophilic bright green fluorescent dye was tested for lipid detection in the microalga Tetraselmis subcordiformis. A concentration of 0.28 μg ml(-1) and staining for 6 min was optimal. Lipid bodies stained with BODIPY505/515 had a characteristic green fluorescence. Their volumes were determined using the sphere volume formula. Lipid accumulation under different nitrogen concentrations was analyzed. With an increase in NaNO(3) concentration from 0 to 240 mg L(-1), the maximum algal concentration increased from 8.23 ± 0.62 (× 10(5) cells ml(-1)) to 1.61 ± 0.13 (×10(6) cells ml(-1)), while the maximum volume of intracellular neutral lipid decreased from 9.78 ± 1.77 μm(3) cell(-1) to 6.00 ± 0.59 μm(3) cell(-1). A comparison of the lipid contents measured by BODIPY 505/515 staining and the gravimetric method showed a positive correlation coefficient of R(2) = 0.93. BODIPY 505/515 staining is a promising method in lipid quantitation in T. subcordiformis.
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Xu
authors.forename: Zhengquan
authors.initials: Z
authors.lastname: Gao
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Xiao
authors.initials: X
authors.lastname: Fan
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Zhang
authors.forename: Naihao
authors.initials: N
authors.lastname: Ye
authors.forename: Shanli
authors.initials: S
authors.lastname: Mou
authors.forename: Chengwei
authors.initials: C
authors.lastname: Liang
authors.forename: Demao
authors.initials: D
authors.lastname: Li
mesh_terms: D000704:Analysis of Variance; D001896:Boron Compounds / Q000378:metabolism*; D000460:Chlorophyta / Q000737:chemistry* / Q000187:drug effects / Q000254:growth & development; D005456:Fluorescent Dyes / Q000378:metabolism*; D006652:Histological Techniques / Q000379:methods*; D008055:Lipids / Q000032:analysis*; D009584:Nitrogen / Q000494:pharmacology
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D008055:Lipids; D009584:Nitrogen