title: Inhibition of a basal transcription factor 3-like gene Osj10gBTF3 in rice results in significant plant miniaturization and typical pollen abortion.
abstract: BTF3, which was originally recognized as a basal transcription factor, has been known to be involved in transcription initiation, translational regulation and protein localization in many eukaryotic organisms. However, its function remains largely unknown in plant species. In the present study, we analyzed a BTF3-related sequence in Oryza sativa L. subsp. japonica, which shares the conserved domain of a nascent polypeptide-associated complex with human BTF3, and was referred to as Osj10gBTF3. The expression of Osj10gBTF3 was primarily constitutive and generally modulated by salt, high temperature and exogenous phytohormone stress. The Osj10gBTF3::EGFP (enhanced green fluorescence protein) fusion protein was localized in both the nucleus and cytoplasmic membrane system. Inhibition of Osj10gBTF3 led to significant morphological changes in all detected tissues and organs, with a reduced size of between 25% and 52%. Furthermore, the pollen that developed was completely sterile, which was correlated with the altered expression of two Rf (fertility restorer)-like genes that encode pentatricopeptide repeat-containing proteins OsPPR676 and OsPPR920, translational initiation factors OseIF3e and OseIF3h, and the heat shock protein OsHSP82. These findings were verified through a yeast two-hybrid assay using a Nipponbare callus cDNA library as bait followed by the reverse transcription-PCR analysis of total leaf or anther RNAs. Our demonstration of the important role of Osj10gBTF3 in rice growth and development provides new insights showing that more complex regulatory functions are associated with BTF3 in plants.
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Zhang
authors.forename: Sunjie
authors.initials: S
authors.lastname: Lu
authors.forename: Mugui
authors.initials: M
authors.lastname: Wang
authors.forename: Liangchao
authors.initials: L
authors.lastname: Wang
authors.forename: Wenyi
authors.initials: W
authors.lastname: Wang
authors.forename: Fangliang
authors.initials: F
authors.lastname: Cao
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Jiwen
authors.initials: J
authors.lastname: Zhang
authors.forename: Jumin
authors.initials: J
authors.lastname: Tu
mesh_terms: D018506:Gene Expression Regulation, Plant; D006358:Hot Temperature; D008904:Miniaturization; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D009928:Organ Specificity; D012275:Oryza / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D010641:Phenotype; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D030821:Plants, Genetically Modified; D011058:Pollen / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D034622:RNA Interference; D011993:Recombinant Fusion Proteins; D012492:Salts; D036226:Seedlings / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D009687:Nuclear Proteins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D012492:Salts; D014157:Transcription Factors; C063552:transcription factor BTF3