title: Notch pathway is involved in high glucose-induced apoptosis in podocytes via Bcl-2 and p53 pathways.
abstract: Recent studies have shown that Notch pathway plays a key role in the pathogenesis of diabetic nephropathy (DN), however, the exact mechanisms remain elusive. Here we demonstrated that high glucose (HG) upregulated Notch pathway in podocytes accompanied with the alteration of Bcl-2 and p53 pathways, subsequently leading to podocytes apoptosis. Inhibition of Notch pathway by chemical inhibitor or specific short hairpin RNA (shRNA) vector in podocytes prevented Bcl-2- and p53-dependent cell apoptosis. These findings suggest that Notch pathway mediates HG-induced podocytes apoptosis via Bcl-2 and p53 pathways.
authors.affiliation: Department of Pathology, Hebei Medical University, Shijiazhuang, Hebei 050017, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Min
authors.initials: M
authors.lastname: Yao
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Shi
authors.forename: Jun
authors.initials: J
authors.lastname: Hao
authors.forename: Yunzhuo
authors.initials: Y
authors.lastname: Ren
authors.forename: Qingjuan
authors.initials: Q
authors.lastname: Liu
authors.forename: Xiaomeng
authors.initials: X
authors.lastname: Wang
authors.forename: Huijun
authors.initials: H
authors.lastname: Duan
mesh_terms: D053829:Amyloid Precursor Protein Secretases / Q000037:antagonists & inhibitors / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002135:Calcium-Binding Proteins / Q000378:metabolism; D055785:Gene Knockdown Techniques; D005947:Glucose / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D050199:Podocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D011480:Protease Inhibitors / Q000494:pharmacology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism; D051880:Receptors, Notch / Q000378:metabolism*; D000072099:Serrate-Jagged Proteins; D015398:Signal Transduction / Q000187:drug effects*; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D002135:Calcium-Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D011480:Protease Inhibitors; D019253:Proto-Oncogene Proteins c-bcl-2; D034741:RNA, Small Interfering; D051880:Receptors, Notch; D000072099:Serrate-Jagged Proteins; D016159:Tumor Suppressor Protein p53; D053829:Amyloid Precursor Protein Secretases; D005947:Glucose