title: Fine mapping of foxglove aphid (Aulacorthum solani) resistance gene Raso1 in soybean and its effect on tolerance to Soybean dwarf virus transmitted by foxglove aphid.
abstract: Soybean dwarf virus (SbDV) causes serious dwarfing, yellowing and sterility in soybean (Glycine max). The soybean cv. Adams is tolerant to SbDV infection in the field and exhibits antibiosis to foxglove aphid (Aulacorthum solani), which transmits SbDV. This antibiosis (termed "aphid resistance") is required for tolerance to SbDV in the field in segregated progenies of Adams. A major quantitative trait locus, Raso1, is reported for foxglove aphid resistance. Our objectives were to fine map Raso1 and to reveal whether Raso1 alone is sufficient to confer both aphid resistance and SbDV tolerance. We introduced Raso1 into cv. Toyomusume by backcrossing and investigated the degree of aphid antibiosis to foxglove aphid and the degree of tolerance to SbDV in the field. All Raso1-introduced backcross lines showed aphid resistance. Interestingly, only one Raso1-introduced backcross line (TM-1386) showed tolerance to SbDV in the field. The results demonstrated Raso1 alone is sufficient to confer aphid resistance but insufficient for SbDV tolerance. Tolerance to SbDV was indicated to require additional gene(s) to Raso1. Additionally, Raso1 was mapped to a 63-kb interval on chromosome 3 of the Williams 82 sequence assembly (Glyma1). This interval includes a nucleotide-binding site-leucine-rich repeat encoding gene and two other genes in the Williams 82 soybean genome sequence.
authors.affiliation: Hokkaido Research Organization Central Agricultural Experiment Station , Naganuma, Yubari, Hokkaido 069-1395, Japan.
authors.forename: Shizen
authors.initials: S
authors.lastname: Ohnishi
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Miyake
authors.forename: Toru
authors.initials: T
authors.lastname: Takeuchi
authors.forename: Fumiko
authors.initials: F
authors.lastname: Kousaka
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hiura
authors.forename: Osamu
authors.initials: O
authors.lastname: Kanehira
authors.forename: Miki
authors.initials: M
authors.lastname: Saito
authors.forename: Takashi
authors.initials: T
authors.lastname: Sayama
authors.forename: Ayako
authors.initials: A
authors.lastname: Higashi
authors.forename: Masao
authors.initials: M
authors.lastname: Ishimoto
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Shohei
authors.initials: S
authors.lastname: Fujita
keywords: NBS-LRR; aphid resistance; foxglove aphid; soybean; soybean dwarf virus