title: Characterization of white spot syndrome virus immediate-early gene promoters.
abstract: Twenty-one immediate-early (IE) genes of white spot syndrome virus (WSSV) have been identified so far. However, the transcriptional regulation of WSSV IE genes remains largely unknown. In this report, the 5' flanking regions of 18 WSSV IE genes were cloned and eight functional promoter regions were identified. WSSV IE gene promoters normally contained a TATA box approximately 30 bp upstream of the transcriptional initiation site. Also, the cyclic AMP response element (CRE; TGACGTCA) was frequently found within the WSSV IE promoter regions. Mutations of the CREs of WSSV IE promoters P403 and P465 reduced their activity significantly, suggesting that these elements have a role in WSSV IE gene transcription. Our findings provide a more global view of WSSV IE gene promoters and will facilitate the in-depth investigation of viral gene transcriptional regulation.
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration (SOA), Xiamen 361005, PR China.|School of Life Science, Xiamen University, Xiamen 361005, PR China.
authors.forename: Fanyu
authors.initials: F
authors.lastname: Lin
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration (SOA), Xiamen 361005, PR China.|School of Life Science, Xiamen University, Xiamen 361005, PR China.
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration (SOA), Xiamen 361005, PR China.|School of Life Science, Xiamen University, Xiamen 361005, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ke
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration (SOA), Xiamen 361005, PR China.
authors.forename: Luhong
authors.initials: L
authors.lastname: Hou
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration (SOA), Xiamen 361005, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration (SOA), Xiamen 361005, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yang
mesh_terms: D001665:Binding Sites; D003001:Cloning, Molecular; D017781:Genes, Immediate-Early; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D024363:Transcription Initiation Site; D046848:White spot syndrome virus 1 / Q000235:genetics*