title: Antihyperglycemic, antilipid peroxidation, and insulin secretory activities of Otostegia persica shoot extract in streptozotocin-induced diabetic rats and in vitro C187 pancreatic β-cells.
abstract: BACKGROUND
  Otostegia persica Boiss (Lamiaceae) contains antioxidant agents and is used in traditional medicine for treatment of diabetes mellitus complications.
  
  
  OBJECTIVE
  The acute antihyperglycemic, antilipid peroxidation, and insulin secretory activities of methanol extract of O. persica aerial parts were investigated.
  
  
  METHODS
  The extract [200, 300, 400 mg/kg body weight (b.w.)] was given orally to rats and glucose (2 g/kg b.w. orally) was administered 30 min later. Glucose and insulin serum levels were measured before and 30, 60, 120, and 240 min after administration of the test samples in normal and diabetic rats. The in vitro insulin secretory activity of extract was evaluated in C187 pancreatic β-cells and its antilipid peroxidation effect was determined by measuring malondialdehyde (MDA) and glutathione (GSH) levels in rat livers after 240 min. The identification of the major phytoconstituents of the extract was carried out using gas chromatography-mass spectrometry.
  
  
  RESULTS
  The extract (300 mg/kg b.w.) significantly decreased the serum glucose level in diabetic rats at 1 h (494 ± 13.4 vs. 426 ± 12.9), 2 h (472.8 ± 17.8 vs. 396 ± 22), and 4 h (438.8 ± 25 vs. 346 ± 19) after treatment. Accordingly, the serum insulin level increased at the same times. The extract significantly increased glucose-induced insulin secretion in C187 β-cells. Moreover, the extract significantly decreased MDA and increased GSH levels in the liver of diabetic rats. Phytochemical analysis revealed thymol as the major phytoconstituent in the extract.
  
  
  CONCLUSIONS
  O. persica shoot extract has antihyperglycemic, antilipid peroxidation, and insulin secretory properties.
authors.affiliation: Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.
authors.forename: Asma
authors.initials: A
authors.lastname: Manzari-Tavakoli
authors.forename: Iran
authors.initials: I
authors.lastname: Pouraboli
authors.forename: Mohamad-Mahdi
authors.initials: MM
authors.lastname: Yaghoobi
authors.forename: Mitra
authors.initials: M
authors.lastname: Mehrabani
authors.forename: Sayed-Mansoor
authors.initials: SM
authors.lastname: Mirtadzadini
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D001786:Blood Glucose / Q000187:drug effects*; D045744:Cell Line, Tumor; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D004305:Dose-Response Relationship, Drug; D008401:Gas Chromatography-Mass Spectrometry; D005978:Glutathione / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D007328:Insulin / Q000097:blood / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism; D019686:Lamiaceae / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D000432:Methanol / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D018520:Plant Shoots; D010946:Plants, Medicinal; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents / Q000737:chemistry; D013311:Streptozocin; D013997:Time Factors
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; D010936:Plant Extracts; D012997:Solvents; D008315:Malondialdehyde; D013311:Streptozocin; D005978:Glutathione; D000432:Methanol