title: A molecular approach (multiplex polymerase chain reaction) for diagnosis of rhinosporidiosis.
abstract: A New multiplex PCR have been developed in our laboratory using primer sets, aiming amplification of both D.N.A target fragments obtained in 18S RNA of commonly encountered fungi in human being and in Rhinosporidium seeberi using F1-fw/F2-rev (500 bp target) and Rhino-fw/ Rhino-rev (.177 bp target). This multiplex PCR has been found to be able to delect R. seeberi from clinical samples and differentiate it from other fungi. Furthermore, by this multiplex PCR, R. seeberi, phylogenitically appears to belong to a member of so called DRIPs clade of fish parasite not a cyanobacterium as claimed previously, by some workers.
authors.affiliation: Department of ENT, Medical College, Calcutta.
authors.forename: Somnath
authors.initials: S
authors.lastname: Saha
authors.forename: Dibyakanti
authors.initials: D
authors.lastname: Mondal
authors.forename: Dimple
authors.initials: D
authors.lastname: Khetawat
authors.forename: Atanu
authors.initials: A
authors.lastname: Roy
authors.forename: Sekhar
authors.initials: S
authors.lastname: Chakrabarti
authors.forename: Basudev
authors.initials: B
authors.lastname: Bhattacharya
keywords: Multiplex PCR; R. seeberi