title: Novel insights into phosgene-induced acute lung injury in rats: role of dysregulated cardiopulmonary reflexes and nitric oxide in lung edema pathogenesis.
abstract: Phosgene gas is a lower respiratory tract irritant. As such, it stimulates nociceptive vagal C-fiber-related reflexes in a dose-rate and concentration × exposure duration (C × t)-dependent manner. In rats, this reflex is characterized by extended apnea time periods, bradycardia, and hypothermia. Although inhalation exposures at nonlethal C × t products show rapid reversibility of reflexively induced changes in respiratory patterns, lethal C × t products seem to cause prolonged stimulation after discontinued exposure to phosgene. This observation has been taken as indirect evidence that phosgene-induced lethal lung edema is likely to be associated with a dysfunctional neurogenic control of cardiopulmonary and microvascular physiology. In order to verify this hypothesis, data from respiratory function measurements during and after the inhalation exposure to phosgene gas were compared with time-course measurements of cardiac function over 20 h post-phosgene exposure. These data were complemented by time-course analyses of nitric oxide (NO(e)) and carbon dioxide in exhaled breath, including time-dependent changes of extravasated protein in bronchoalveolar lavage fluid and hemoglobin in blood. The nitric oxidase synthetase inhibitors L-NAME and L-NIL were used to further elucidate the role of NO(e) in this type of acute lung injury and whether its analysis can serve as an early biomarker of pulmonary injury. Collectively, the sequence and time course of pathological events in phosgene-induced lung edema appear to suggest that overstimulated, continued sensorimotor vagal reflexes affect cardiopulmonary hemodynamics. A continued parasympathetic tone appears to be involved in this etiopathology.
authors.affiliation: Department of Toxicology, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Li
authors.forename: Fangfang
authors.initials: F
authors.lastname: Liu
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Hubert
authors.initials: H
authors.lastname: Truebel
authors.forename: Juergen
authors.initials: J
authors.lastname: Pauluhn
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced* / Q000503:physiopathology; D000818:Animals; D001831:Body Temperature; D001944:Breath Tests; D004562:Electrocardiography; D006321:Heart / Q000187:drug effects* / Q000503:physiopathology; D006339:Heart Rate; D008168:Lung / Q000187:drug effects* / Q000503:physiopathology; D008297:Male; D009569:Nitric Oxide / Q000502:physiology*; D010705:Phosgene / Q000633:toxicity*; D011654:Pulmonary Edema / Q000139:chemically induced* / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010705:Phosgene; D009569:Nitric Oxide