title: Effects of Rehmannia glutinosa oligosaccharide on human adipose-derived mesenchymal stem cells in vitro.
abstract: OBJECTIVE
  Adipose-derived mesenchymal stem cells (ADMSCs) are considered as a good cell source for regenerative medicine with their self-renew capacity, multilineage differentiation and immunomodulatory potency. Based on this background, the aim of this study was to evaluate the influence of Rehmannia glutinosa oligosaccharide (RGO), a traditional Chinese medicine, on human ADMSCs' proliferation, H(2)O(2)-induced apoptosis, and secretion of Vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) in vitro.
  
  
  METHODS
  Human ADMSCs were isolated and cultured in vitro. Then flow cytometry was carried out to characterize the cells, and MTT assay was performed to detect the proliferation. H(2)O(2)-induced apoptosis was evaluated by flow cytometry. VEGF and HGF production were detected by ELISA kits.
  
  
  RESULTS
  Human ADMSCs were positive for CD90 and CD29, but negative for CD31, CD34 and CD45. The results also indicate that RGO can promote the proliferation and alleviate H(2)O(2)-induced apoptosis of human ADMSCs. The mechanism of RGO's protective effect may involve the up-regulation of VEGF and HGF.
  
  
  CONCLUSIONS
  The present study indicates that RGO may increase the viability and proliferative capacity and alleviate H(2)O(2)-induced apoptosis of human ADMSCs via the paracrine release of VEGF and HGF. These results indicate that RGO application will enhance stem cell viability and improve their effects in cell therapy.
authors.affiliation: Department of Cardiology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yihong
authors.initials: Y
authors.lastname: Qin
authors.forename: Ting
authors.initials: T
authors.lastname: Chen
authors.forename: Weidong
authors.initials: W
authors.lastname: Han
authors.forename: Guanghui
authors.initials: G
authors.lastname: Chen
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000328:Adult; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008516:Medicine, Chinese Traditional; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism; D009844:Oligosaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D032267:Rehmannia / Q000737:chemistry*; D015854:Up-Regulation / Q000187:drug effects; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D009844:Oligosaccharides; D042461:Vascular Endothelial Growth Factor A; D017228:Hepatocyte Growth Factor; D006861:Hydrogen Peroxide