title: New secondary metabolites from bioactive extracts of the fungus Armillaria tabescens.
abstract: Ethyl acetate extracts of Armillaria tabescens (strain JNB-OZ344) showed significant fungistatic and bacteristatic activities against several major human pathogens including Candida albicans, Cryptococcus neoformans, Escherichia coli and Mycobacterium intracellulare. Chemical analysis of these extracts led to the isolation and identification of four new compounds, emestrin-F (1), emestrin-G (2), 6-O-(4-O-methyl-β-D-glucopyranosyl)-8-hydroxy-2,7-dimethyl-4H-benzopyran-4-one (3) and cephalosporolide-J (4), along with five other previously known compounds, emestrin (5), cephalosporolide-E (6), decarestrictine-C2 (7), ergosterol and brassicasterol. Structural elucidation of all compounds was carried out by NMR and MS analyses. Antimicrobial assays revealed that compounds 1 and 5 were responsible for the observed growth inhibitory activities of the fungal extracts against the human pathogens tested.
authors.affiliation: National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
authors.forename: H M T Bandara
authors.initials: HM
authors.lastname: Herath
authors.forename: Melissa
authors.initials: M
authors.lastname: Jacob
authors.forename: A Dan
authors.initials: AD
authors.lastname: Wilson
authors.forename: Hamed K
authors.initials: HK
authors.lastname: Abbas
authors.forename: N P Dhammika
authors.initials: NP
authors.lastname: Nanayakkara
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D000935:Antifungal Agents / Q000737:chemistry* / Q000494:pharmacology; D055392:Armillaria / Q000737:chemistry*; D002176:Candida albicans / Q000187:drug effects; D002775:Cholestadienols / Q000737:chemistry / Q000494:pharmacology; D003455:Cryptococcus neoformans / Q000187:drug effects; D004875:Ergosterol / Q000737:chemistry / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D010840:Phytosterols / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D002775:Cholestadienols; D010840:Phytosterols; C003532:brassicasterol; D004875:Ergosterol