title: Reversing effect of ring finger protein 43 inhibition on malignant phenotypes of human hepatocellular carcinoma.
abstract: It has been shown that Ring finger protein 43 (RNF43) is overexpressed in colorectal cancer and mediates cancer cell proliferation; however, its role in hepatocellular carcinoma (HCC) remains unknown. In this study, we found that RNF43 was frequently overexpressed in HCCs, and this overexpression was correlated with positive vascular invasion, poor tumor differentiation, and advanced tumor stage. Functional studies showed that knockdown of RNF43 could induce apoptosis and inhibit proliferation, invasion, colony formation, and xenograft growth of HCCs. Microarray-based gene profiling showed a total of 229 genes differentially expressed after RNF43 knockdown, many of which are involved in oncogenic processes such as cell proliferation, cell adhesion, cell motility, cell death, DNA repair, and so on. These results suggest that RNF43 is involved in tumorigenesis and progression of HCCs and that antagonism of RNF43 may be beneficial for HCC treatment.
authors.affiliation: Key Lab of Combined Multi-organ Transplantation, Ministry of Public Health, Hangzhou, Zhejiang, China.
authors.forename: Chunyang
authors.initials: C
authors.lastname: Xing
authors.forename: Wuhua
authors.initials: W
authors.lastname: Zhou
authors.forename: Songming
authors.initials: S
authors.lastname: Ding
authors.forename: Haiyang
authors.initials: H
authors.lastname: Xie
authors.forename: Wu
authors.initials: W
authors.lastname: Zhang
authors.forename: Zhe
authors.initials: Z
authors.lastname: Yang
authors.forename: Bajin
authors.initials: B
authors.lastname: Wei
authors.forename: Kangjie
authors.initials: K
authors.lastname: Chen
authors.forename: Rong
authors.initials: R
authors.lastname: Su
authors.forename: Jun
authors.initials: J
authors.lastname: Cheng
authors.forename: Shusen
authors.initials: S
authors.lastname: Zheng
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D002471:Cell Transformation, Neoplastic / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009368:Neoplasm Transplantation; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D034741:RNA, Small Interfering / Q000235:genetics; D059467:Transcriptome / Q000187:drug effects; D047368:Tumor Burden; D044767:Ubiquitin-Protein Ligases; D055815:Young Adult
chemical_list: D004268:DNA-Binding Proteins; D015513:Oncogene Proteins; D034741:RNA, Small Interfering; C496945:RNF43 protein, human; D044767:Ubiquitin-Protein Ligases; D020782:Matrix Metalloproteinases