title: Antibody response to Mycobacterium tuberculosis p27-PPE36 antigen in sera of pulmonary tuberculosis patients.
abstract: We have expressed, characterized and studied the immune response of mice against the Mycobacterium tuberculosis PPE36 protein. This protein encoded by the gene Rv2108 is a 27-kDa cell-wall associated protein. Here, we compare the antibody isotypes distribution of p27-PPE36 in sera of pulmonary tuberculosis patients and in sera from healthy control subjects by enzyme-linked immunosorbent assay (ELISA). We detected in the sera of affected individuals a significant increase of IgA antibody response, a no significant augmentation of IgM response, a complete lack of IgG2, 3, and 4 responses and a weak IgG1 response in a few subjects.
authors.affiliation: UBO, IUT de Quimper, 2, rue de l'Université, 29334 Quimper, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Le Moigne
authors.forename: Delphine
authors.initials: D
authors.lastname: Le Moigne
authors.forename: Wahib
authors.initials: W
authors.lastname: Mahana
mesh_terms: D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000097:blood; D000942:Antigens, Bacterial / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000097:blood; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007070:Immunoglobulin A / Q000096:biosynthesis / Q000097:blood; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis / Q000097:blood; D008074:Lipoproteins / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D008074:Lipoproteins; C110121:P27 protein, Mycobacterium; C583539:PPE36 protein, Mycobacterium tuberculosis