title: PBSIM: PacBio reads simulator--toward accurate genome assembly.
abstract: BACKGROUND
  PacBio sequencers produce two types of characteristic reads (continuous long reads: long and high error rate and circular consensus sequencing: short and low error rate), both of which could be useful for de novo assembly of genomes. Currently, there is no available simulator that targets the specific generation of PacBio libraries.
  
  
  RESULTS
  Our analysis of 13 PacBio datasets showed characteristic features of PacBio reads (e.g. the read length of PacBio reads follows a log-normal distribution). We have developed a read simulator, PBSIM, that captures these features using either a model-based or sampling-based method. Using PBSIM, we conducted several hybrid error correction and assembly tests for PacBio reads, suggesting that a continuous long reads coverage depth of at least 15 in combination with a circular consensus sequencing coverage depth of at least 30 achieved extensive assembly results.
  
  
  BACKGROUND
  PBSIM is freely available from the web under the GNU GPL v2 license (http://code.google.com/p/pbsim/).
authors.affiliation: Information and Mathematical Science and Bioinformatics Co., Ltd., Toshima-ku, Tokyo 170-0013, Japan.
authors.forename: Yukiteru
authors.initials: Y
authors.lastname: Ono
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Asai
authors.forename: Michiaki
authors.initials: M
authors.lastname: Hamada
mesh_terms: D023281:Genomics / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods; D012984:Software