title: Characterization of atherosclerotic plaque depositions by Raman and FTIR imaging.
abstract: Spectroscopy-based imaging techniques can provide useful biochemical information about tissue samples. Here, we employ Raman and Fourier transform infrared (IR) imaging to characterize composition and constitution of atherosclerotic plaques of rabbits, fed with a high cholesterol diet. The results were compared with conventional light microscopy after staining with hematoxylin eosin, and elastica van Gieson. The spectral unmixing algorithm vertex component analysis was applied for data analysis and image reconstruction. IR microscopy allowed for differentiation between lipids and proteins in plaques of full aortic cross sections. Raman microscopy further discriminated cholesterol esters, cholesterol and triglycerides. FTIR and Raman images were recorded at a resolution near 20 micrometer per pixel for a large field of view. High resolution Raman images at 1 micrometer per pixel revealed structural details at selected regions of interest. The intima-media and the lipid-protein ratio were determined in five specimens for quantitation. These results correlate well with histopathology. The described method is a promising tool for easy and fast molecular imaging of atherosclerosis.
authors.affiliation: University Hospital-Friedrich-Schiller-University of Jena, Clinic for Internal Medicine I, Jena, Germany.
authors.forename: Annika
authors.initials: A
authors.lastname: Lattermann
authors.forename: Christian
authors.initials: C
authors.lastname: Matthäus
authors.forename: Norbert
authors.initials: N
authors.lastname: Bergner
authors.forename: Claudia
authors.initials: C
authors.lastname: Beleites
authors.forename: Bernd F
authors.initials: BF
authors.lastname: Romeike
authors.forename: Christoph
authors.initials: C
authors.lastname: Krafft
authors.forename: Bernhard R
authors.initials: BR
authors.lastname: Brehm
authors.forename: Jürgen
authors.initials: J
authors.lastname: Popp
mesh_terms: D000465:Algorithms; D000818:Animals; D001013:Aorta, Thoracic / Q000473:pathology; D050197:Atherosclerosis / Q000378:metabolism; D059168:Carotid Intima-Media Thickness; D003952:Diagnostic Imaging / Q000379:methods; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008055:Lipids / Q000737:chemistry; D008297:Male; D015233:Models, Statistical; D058226:Plaque, Atherosclerotic / Q000175:diagnosis / Q000473:pathology*; D011817:Rabbits; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014280:Triglycerides / Q000737:chemistry
chemical_list: D008055:Lipids; D014280:Triglycerides