title: Enzymatic assay for D-aspartic acid using D-aspartate oxidase and oxaloacetate decarboxylase.
abstract: An enzymatic assay system for D-Asp was established using D-aspartate oxidase and oxaloacetate decarboxylase. In this system, D-Asp is converted to pyruvate, which is determined fluorometrically with 1,2-diamino-4,5-methylenedioxybenzene. This method makes possible D-Asp measurement at the micromolar level. The D-Asp contents of an edible brown alga, Hijika fusiforme, a lactic acid bacteria beverage, and pig testis were determined by the method.
authors.affiliation: Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan.
authors.forename: Shiro
authors.initials: S
authors.lastname: Kato
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Ikuta
authors.forename: Hisashi
authors.initials: H
authors.lastname: Hemmi
authors.forename: Shoji
authors.initials: S
authors.lastname: Takahashi
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kera
authors.forename: Tohru
authors.initials: T
authors.lastname: Yoshimura
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism*; D002262:Carboxy-Lyases / Q000378:metabolism*; D050804:D-Aspartate Oxidase / Q000378:metabolism*; D057075:Enzyme Assays / Q000379:methods*; D008297:Male; D000459:Phaeophyceae / Q000201:enzymology; D013552:Swine; D013737:Testis / Q000201:enzymology
chemical_list: D001224:Aspartic Acid; D050804:D-Aspartate Oxidase; D002262:Carboxy-Lyases; C021916:oxaloacetate decarboxylase