title: Genetic diversity of Burkholderia contaminans isolates from cystic fibrosis patients in Argentina.
abstract: A total of 120 Burkholderia cepacia complex isolates collected during 2004-2010 from 66 patients in two cystic fibrosis reference centers in Argentina were analyzed. Burkholderia contaminans was the species most frequently recovered (57.6%), followed by Burkholderia cenocepacia (15%), a species distribution not reported so far. The recA-PCR-based techniques applied to the B. contaminans isolates revealed that 85% of the population carried the recA-ST-71 allele. Our results showed the utility of BOX-PCR genotyping in analyzing B. contaminans diversity. This approach allowed us to address clonal transmission during an outbreak and the genetic changes occurring in infecting bacteria over the course of chronic infection.
authors.affiliation: Centro de Investigación y Desarrollo de Fermentaciones Industriales (CINDEFI-CONICET, CCT-La Plata), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina.
authors.forename: Pablo
authors.initials: P
authors.lastname: Martina
authors.forename: Marisa
authors.initials: M
authors.lastname: Bettiol
authors.forename: Cecilia
authors.initials: C
authors.lastname: Vescina
authors.forename: Patricia
authors.initials: P
authors.lastname: Montanaro
authors.forename: M Constanza
authors.initials: MC
authors.lastname: Mannino
authors.forename: Claudia I
authors.initials: CI
authors.lastname: Prieto
authors.forename: Carlos
authors.initials: C
authors.lastname: Vay
authors.forename: Dieter
authors.initials: D
authors.lastname: Naumann
authors.forename: Juergen
authors.initials: J
authors.lastname: Schmitt
authors.forename: Osvaldo
authors.initials: O
authors.lastname: Yantorno
authors.forename: Antonio
authors.initials: A
authors.lastname: Lagares
authors.forename: Alejandra
authors.initials: A
authors.lastname: Bosch
mesh_terms: D001118:Argentina; D015373:Bacterial Typing Techniques; D019121:Burkholderia Infections / Q000382:microbiology*; D042602:Burkholderia cepacia complex / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D003550:Cystic Fibrosis / Q000150:complications*; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D058889:Molecular Typing; D016133:Polymerase Chain Reaction; D011938:Rec A Recombinases / Q000235:genetics
chemical_list: D011938:Rec A Recombinases