title: Analysis of fatty alcohol derivatives with comprehensive two-dimensional liquid chromatography coupled with mass spectrometry.
abstract: A simultaneous separation of anionic (fatty alcohol sulfates, fatty alcohol ether sulfates), non-ionic (alkyl polyglucosides, fatty alcohol ethoxylates) and amphoteric (cocamidopropyl betaines) surfactants was performed by comprehensive two-dimensional liquid chromatography (LCxLC) utilizing a ZIC(®)-HILIC column in the first dimension, a Reprosphere 100 C8-Aqua column in the second dimension and a 10-port two position valve as the interface. The volume of the two sample loops were 25 or 50 μL and allow a one or two minute modulation at a 25 μL/min flow rate. In the first dimension, a gradient of acetonitrile and an ammonium acetate buffer was used to separate polyethoxylated surfactants by their degree of ethoxylation (EO number) whereas in the second dimension, a separation by alkyl chain was performed using a methanol/ammonium acetate buffer gradient. A baseline separation of the above mentioned surfactants according to both EO number and alkyl chain was achieved. The best performance was used to compare two different LCxLC-QTOF MS systems, which demonstrate that a transfer of the method from one system to a totally different system is possible. However, because of the differences in delay volume and extra-column volume between these systems the separation power is changed.
authors.affiliation: Institute for Pure and Applied Mass Spectrometry, University of Wuppertal, Gaussstr. 20, 42119 Wuppertal, Germany.
authors.forename: Victoria
authors.initials: V
authors.lastname: Elsner
authors.forename: Sabrina
authors.initials: S
authors.lastname: Laun
authors.forename: David
authors.initials: D
authors.lastname: Melchior
authors.forename: Michael
authors.initials: M
authors.lastname: Köhler
authors.forename: Oliver J
authors.initials: OJ
authors.lastname: Schmitz
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D005233:Fatty Alcohols / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure
chemical_list: D005233:Fatty Alcohols