title: Separation and purification of sulforaphene from radish seeds using macroporous resin and preparative high-performance liquid chromatography.
abstract: This present study described a rapid and cost-effective method for the separation and purification of natural sulforaphene from radish seeds by SP-700 macroporous resin and preparative high-performance liquid chromatography (HPLC). Sulforaphene with high purity and recovery was obtained by preparative HPLC with a C18 column and 30% methanol in ultra-pure water as the mobile phase. 12.5 kg of radish seeds, which contained 87.5 g of sulforaphene, produced 117.5 g of sulforaphene-rich extract of 65.8% sulforaphene after primary separation by SP-700 macroporous resin. 5.9 g of 96.5% sulforaphene was obtained from 9.5 g of the sulforaphene-rich extract after purification by preparative HPLC. The purified compound was assessed by analytical HPLC and characterised by ESI/MS, (1)H NMR and (13)C NMR. Standard curve was developed using the purified sulforaphene to allow quantification of sulforaphene in the extracts of radish seeds by analytical HPLC.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Pengqun
authors.initials: P
authors.lastname: Kuang
authors.forename: Dan
authors.initials: D
authors.lastname: Song
authors.forename: Qipeng
authors.initials: Q
authors.lastname: Yuan
authors.forename: Rui
authors.initials: R
authors.lastname: Yi
authors.forename: Xinhua
authors.initials: X
authors.lastname: Lv
authors.forename: Hao
authors.initials: H
authors.lastname: Liang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D017879:Isothiocyanates / Q000302:isolation & purification*; D010936:Plant Extracts / Q000302:isolation & purification*; D016062:Porosity; D031224:Raphanus / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry; D012639:Seeds / Q000737:chemistry*
chemical_list: D017879:Isothiocyanates; D010936:Plant Extracts; D012117:Resins, Synthetic; C473643:sulphoraphene