title: A new virus from the plant pathogenic oomycete Phytophthora infestans with an 8 kb dsRNA genome: the sixth member of a proposed new virus genus.
abstract: A virus designated Phytophthora infestans RNA virus 3 (PiRV-3) was characterized from an isolate of P. infestans that was co-infected with a second previously described virus, PiRV-4, a member of the virus family Narnaviridae (Cai et al., 2012). The genome of PiRV-3 is 8112 nt and one strand, designated the positive strand, has two deduced overlapping open reading frames linked by a potential frameshift sequence. The first open reading frame (ORF1) is predicted to encode a protein of unknown function, and ORF2 is predicted to encode an RNA-dependent RNA polymerase (RdRp) most closely related to six unclassified dsRNA viruses of filamentous fungi. The genome organizations of five of the related viruses are similar to PiRV-3, indicating taxonomic linkage among those viruses. We suggest that PiRV-3 and related viruses should be collected into a new virus taxon.
authors.affiliation: Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA. cai@aesop.rutgers.edu
authors.forename: Guohong
authors.initials: G
authors.lastname: Cai
authors.forename: Jason F
authors.initials: JF
authors.lastname: Krychiw
authors.forename: Kevin
authors.initials: K
authors.lastname: Myers
authors.forename: William E
authors.initials: WE
authors.lastname: Fry
authors.forename: Bradley I
authors.initials: BI
authors.lastname: Hillman
mesh_terms: D019295:Computational Biology; D016679:Genome, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D009868:Oomycetes / Q000145:classification / Q000821:virology*; D016366:Open Reading Frames; D010641:Phenotype; D055750:Phytophthora infestans / Q000821:virology*; D010935:Plant Diseases / Q000821:virology*; D012328:RNA Viruses / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D011198:Solanum tuberosum / Q000821:virology*; D013045:Species Specificity