title: Comprehensive and high-resolution typing of swine leukocyte antigen DQA from genomic DNA and determination of 25 new SLA class II haplotypes.
abstract: We previously reported the development of genomic-DNA-based high-resolution genotyping methods for SLA-DQB1 and DRB1. Here, we report the successful typing of SLA-DQA using similar methodological principles. We designed a method for comprehensive genotyping of SLA-DQA using intronic sequence information of SLA-DQA exon 2 that we had obtained from 12 animals with different SLA-DQB1 genotypes. We expanded our typing to 76 selected animals with diverse DQB1 and DRB1 genotypes, 140 random animals from 7 pig breeds, and 3 wild boars. This resulted in the identification of 17 DQA alleles with 49 genotypes. Two new alleles were identified from wild boars. Combine with SLA-DQB1, and DRB1 typing results, we identified 34 SLA class II haplotypes including 25 that were previously unreported.
authors.affiliation: Department of Animal Biotechnology, Konkuk University, Seoul, South Korea.
authors.forename: M T
authors.initials: MT
authors.lastname: Le
authors.forename: H
authors.initials: H
authors.lastname: Choi
authors.forename: M-K
authors.initials: MK
authors.lastname: Choi
authors.forename: D T
authors.initials: DT
authors.lastname: Nguyen
authors.forename: J-H
authors.initials: JH
authors.lastname: Kim
authors.forename: H G
authors.initials: HG
authors.lastname: Seo
authors.forename: S-Y
authors.initials: SY
authors.lastname: Cha
authors.forename: K
authors.initials: K
authors.lastname: Seo
authors.forename: T
authors.initials: T
authors.lastname: Chun
authors.forename: L B
authors.initials: LB
authors.lastname: Schook
authors.forename: C
authors.initials: C
authors.lastname: Park
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D005802:Genes, MHC Class II; D060005:Genotyping Techniques / Q000379:methods; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000235:genetics* / Q000276:immunology*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; C438668:swine leukocyte antigen