title: Synthase-dependent exopolysaccharide secretion in Gram-negative bacteria.
abstract: The biosynthesis and export of bacterial cell-surface polysaccharides is known to occur through several distinct mechanisms. Recent advances in the biochemistry and structural biology of several proteins in synthase-dependent polysaccharide secretion systems have identified key conserved components of this pathway in Gram-negative bacteria. These components include an inner-membrane-embedded polysaccharide synthase, a periplasmic tetratricopeptide repeat (TPR)-containing scaffold protein, and an outer-membrane β-barrel porin. There is also increasing evidence that many synthase-dependent systems are post-translationally regulated by the bacterial second messenger bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP). Here, we compare these core proteins in the context of the alginate, cellulose, and poly-β-D-N-acetylglucosamine (PNAG) secretion systems.
authors.affiliation: Program in Molecular Structure and Function, The Hospital for Sick Children, 555 University Avenue, Toronto, ON M5G 1X8, Canada.
authors.forename: J C
authors.initials: JC
authors.lastname: Whitney
authors.forename: P L
authors.initials: PL
authors.lastname: Howell
mesh_terms: D000464:Alginates; D001426:Bacterial Proteins / Q000378:metabolism*; D058947:Bacterial Secretion Systems / Q000502:physiology; D002482:Cellulose / Q000096:biosynthesis; D020723:Glucuronic Acid / Q000096:biosynthesis; D006090:Gram-Negative Bacteria / Q000378:metabolism*; D006603:Hexuronic Acids; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000378:metabolism*; D047071:beta-Glucans / Q000378:metabolism
chemical_list: D000464:Alginates; D001426:Bacterial Proteins; D058947:Bacterial Secretion Systems; D006603:Hexuronic Acids; D011135:Polysaccharides, Bacterial; D047071:beta-Glucans; C505465:poly-N-acetyl-1-6-glucosamine; D020723:Glucuronic Acid; D002482:Cellulose