title: A maternally inherited DNA marker, descended from Solanum demissum (2n = 6x = 72) to S. tuberosum (2n = 4x = 48).
abstract: A Mexican hexaploid wild potato species, Solanum demissum (dms), was only used as a female in previous breeding programs. The resulting clones with dms cytoplasm produced abundant, but non-functional pollen. A 170 bp DNA fragment, named Band 1, was originally detected in the F(1) hybrid between dms and S. tuberosum. In this study, the sequenced region was extended to 1,032 bp; nevertheless, it did not show any homology to known sequences. This extended region harboring Band 1 was, without introns, all transcribed to mRNA and was maternally inherited from dms to S. tuberosum through backcrosses. Three dms accessions, 168 accessions of 38 cultivated and closely related wild species, and 158 varieties and breeding lines were surveyed, which demonstrated that Band 1 was specific to dms and varieties and breeding lines with dms cytoplasm. Thus, Band 1 is a useful marker to distinguish dms cytoplasm, which enables us to design efficient mating combinations in breeding programs.
authors.affiliation: Graduate School of Agricultural Science, Kobe University , Nada, Kobe, Hyogo 657-8501, Japan ; National Agricultural Research Center for Hokkaido Region , Shinsei, Memuro, Hokkaido 082-0081, Japan.
authors.forename: Rena
authors.initials: R
authors.lastname: Sanetomo
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Hosaka
keywords: DNA marker; Solanum demissum; W/α cytoplasm; backcrossing; cytoplasmic male sterility; transcribed region; unknown sequence