title: From specific to global analysis of posttranscriptional regulation in eukaryotes: posttranscriptional regulatory networks.
abstract: Regulation of gene expression occurs at several levels in eukaryotic organisms and is a highly controlled process. Although RNAs have been traditionally viewed as passive molecules in the pathway from transcription to translation, there is mounting evidence that their metabolism is controlled by a class of proteins called RNA-binding proteins (RBPs), as well as a number of small RNAs. In this review, I provide an overview of the recent developments in our understanding of the repertoire of RBPs across diverse model systems, and discuss the computational and experimental approaches currently available for the construction of posttranscriptional networks governed by them. I also present an overview of the different roles played by RBPs in the cellular context, based on their cis-regulatory modules identified in the literature and discuss how their interplay can result in the dynamic, spatial and tissue-specific expression maps of RNAs. I finally present the concept of posttranscriptional network of RBPs and their cognate RNA targets and discuss their cross-talk with other important posttranscriptional regulatory molecules such as microRNAs s, resulting in diverse functional network motifs. I argue that with rapid developments in the genome-wide elucidation of posttranscriptional networks it would not only be possible to gain a deeper understanding of regulation at a level that has been under-appreciated in the past, but would also allow us to use the newly developed high-throughput approaches to interrogate the prevalence of these phenomena in different states, and thereby study their relevance to physiology and disease across organisms.
authors.affiliation: School of Informatics, Indiana University Purdue University, Indianapolis, Indiana, Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, 719 Indiana Ave Ste 319, Walker Plaza Building, IN 46202, USA. scjanga@iupui.edu
authors.forename: Sarath Chandra
authors.initials: SC
authors.lastname: Janga
mesh_terms: D000818:Animals; D056890:Eukaryota / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics* / Q000502:physiology; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D016601:RNA-Binding Proteins