title: Fibroblast growth factor-21 serum concentrations are associated with metabolic and hepatic markers in humans.
abstract: Rather than a traditional growth factor, fibroblast growth factor-21 (FGF21) is considered to be a metabolic hormone. In the current study, we investigated serum FGF21 levels in the self-contained population of Sorbs. Serum FGF21 concentrations were quantified by ELISA and correlated with IGF1 as well as metabolic, renal, hepatic, inflammatory, and cardiovascular parameters in 913 Sorbs from Germany. Moreover, human IGF1 protein secretion was investigated in FGF21-stimulated HepG2 cells. Median FGF21 serum concentrations were 2.1-fold higher in subjects with type 2 diabetes mellitus (141.8 ng/l) compared with controls (66.7 ng/l). Furthermore, nondiabetic subjects with FGF21 levels below the detection limit of the ELISA showed a more beneficial metabolic profile compared with subjects with measurable FGF21. Moreover, FGF21 was significantly lower in female compared with male subjects after adjustment for age and BMI. In multiple regression analyses, circulating FGF21 concentrations remained independently and positively associated with gender, systolic blood pressure, triglycerides, and γ glutamyl transferase whereas a negative association was observed with IGF1 in nondiabetic subjects. Notably, FGF21 significantly inhibited IGF1 secretion into HepG2 cell culture supernatants in preliminary in vitro experiments. FGF21 serum concentrations are associated with facets of the metabolic syndrome, hepatocellular function, as well as GH status.
authors.affiliation: Medical Department, University of Leipzig, Liebigstrasse 18, 04103 Leipzig, Germany Leipzig University Medical Center, IFB AdiposityDiseases, 04103 Leipzig, Germany.
authors.forename: Susan
authors.initials: S
authors.lastname: Kralisch
authors.forename: Anke
authors.initials: A
authors.lastname: Tönjes
authors.forename: Kerstin
authors.initials: K
authors.lastname: Krause
authors.forename: Judit
authors.initials: J
authors.lastname: Richter
authors.forename: Ulrike
authors.initials: U
authors.lastname: Lossner
authors.forename: Peter
authors.initials: P
authors.lastname: Kovacs
authors.forename: Thomas
authors.initials: T
authors.lastname: Ebert
authors.forename: Matthias
authors.initials: M
authors.lastname: Blüher
authors.forename: Michael
authors.initials: M
authors.lastname: Stumvoll
authors.forename: Mathias
authors.initials: M
authors.lastname: Fasshauer
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D005346:Fibroblast Growth Factors / Q000097:blood*; D056945:Hep G2 Cells; D006801:Humans; D007333:Insulin Resistance / Q000502:physiology*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D010641:Phenotype
chemical_list: C414620:fibroblast growth factor 21; D005346:Fibroblast Growth Factors; D007334:Insulin-Like Growth Factor I