title: [Construction of TDRG1 shRNA expression vector and interfering effect of TDRG1 shRNA expression vector on NTERA-2 cells].
abstract: OBJECTIVE
  To construct short hairpin RNA interfering expression vector of TDRG1,and detect the specific interfering effect of TDRG1-shRNA expression vector on NTERA-2 cells.
  
  
  METHODS
  Oligos for short hairpin RNA targefing for TDRG1 were designed and connected to the expression vector pGPU6/GFP/Neo to construct the TDRG1 shRNA expression vector. The recombinant plasmid TDRG1-shRNA486, TDRG1-shRNA738, TDRG1-shRNA921 and lipofectamine ™2000 were used to generate and transfect shRNA into NTERA-2 cells. Expression of TDRG1 mRNA was assayed by RT-PCR.
  
  
  RESULTS
  TDRG1-shRNA expression vector was successfully constructed. TDRG1-shRNA486 was more effective in the suppression of TDRG1 with significant reduction of TDRG1 mRNA.
  
  
  CONCLUSIONS
  TDRG1-shRNA can interfere the expression of TDRG1 in NTERA-2 cells.
authors.affiliation: Department of Urology, Third Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Shenglin
authors.initials: S
authors.lastname: Peng
authors.forename: Jianfu
authors.initials: J
authors.lastname: Yang
authors.forename: Houyang
authors.initials: H
authors.lastname: Chen
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Guo
authors.forename: Dongjie
authors.initials: D
authors.lastname: Li
authors.forename: Huabo
authors.initials: H
authors.lastname: Zhou
authors.forename: Yu
authors.initials: Y
authors.lastname: Gan
authors.forename: Xianzhen
authors.initials: X
authors.lastname: Jiang
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Tang
mesh_terms: D045744:Cell Line, Tumor; D005822:Genetic Vectors; D006801:Humans; D034622:RNA Interference; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D014162:Transfection
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering