title: A practical quantification method for Heinz bodies in birds applicable to rapid response field scenarios.
abstract: Oil-induced oxidative injury to red blood cells results in Heinz body hemolytic anemia. Here, we evaluated three Heinz body staining techniques in brown pelican (Pelecanus occidentalis) blood. Using a range of in vitro acetylphenylhydrazine incubations, we validated a field-adapted technique against laboratory wet-mounts and verified the stability of this technique for one month following preparation. Employing this technique during petrochemical spill responses allows for delays between sample collection and analysis.
authors.affiliation: Department of Fish and Wildlife Conservation, Virginia Tech, Blacksburg, Virginia, USA. jfallon@vt.edu
authors.forename: Jesse A
authors.initials: JA
authors.lastname: Fallon
authors.forename: William A
authors.initials: WA
authors.lastname: Hopkins
authors.forename: Lee
authors.initials: L
authors.lastname: Fox
mesh_terms: D000743:Anemia, Hemolytic / Q000097:blood / Q000139:chemically induced / Q000662:veterinary; D000818:Animals; D001717:Birds / Q000502:physiology*; D004784:Environmental Monitoring / Q000379:methods*; D004906:Erythrocyte Count; D004912:Erythrocytes; D006366:Heinz Bodies / Q000378:metabolism*; D059392:Petroleum Pollution; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D014874:Water Pollutants, Chemical