title: Phosphatidylethanolamine biosynthesis in mitochondria: phosphatidylserine (PS) trafficking is independent of a PS decarboxylase and intermembrane space proteins UPS1P and UPS2P.
abstract: Phosphatidylethanolamine (PE) plays important roles for the structure and function of mitochondria and other intracellular organelles. In yeast, the majority of PE is produced from phosphatidylserine (PS) by a mitochondrion-located PS decarboxylase, Psd1p. Because PS is synthesized in the endoplasmic reticulum (ER), PS is transported from the ER to mitochondria and converted to PE. After its synthesis, a portion of PE moves back to the ER. Two mitochondrial proteins located in the intermembrane space, Ups1p and Ups2p, have been shown to regulate PE metabolism by controlling the export of PE. It remains to be determined where PS is decarboxylated in mitochondria and whether decarboxylation is coupled to trafficking of PS. Here, using fluorescent PS as a substrate in an in vitro assay for Psd1p-dependent PE production in isolated mitochondria, we show that PS is transferred from the mitochondrial outer membrane to the inner membrane independently of Psd1p, Ups1p, and Ups2p and decarboxylated to PE by Psd1p in the inner membrane. Interestingly, Ups1p is required for the maintenance of Psd1p and therefore PE production. Restoration of Psd1p levels rescued PE production defects in ups1Δ mitochondria. Our data provide novel mechanistic insight into PE biogenesis in mitochondria.
authors.affiliation: Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA. tamura@chem.nagoya-u.ac.jp
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Tamura
authors.forename: Ouma
authors.initials: O
authors.lastname: Onguka
authors.forename: Kie
authors.initials: K
authors.lastname: Itoh
authors.forename: Toshiya
authors.initials: T
authors.lastname: Endo
authors.forename: Miho
authors.initials: M
authors.lastname: Iijima
authors.forename: Steven M
authors.initials: SM
authors.lastname: Claypool
authors.forename: Hiromi
authors.initials: H
authors.lastname: Sesaki
mesh_terms: D001693:Biological Transport, Active / Q000502:physiology; D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D051336:Mitochondrial Membranes / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000096:biosynthesis* / Q000235:genetics; D010718:Phosphatidylserines / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D024101:Mitochondrial Proteins; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D029701:Saccharomyces cerevisiae Proteins; C512497:Ups1 protein, S cerevisiae; C542159:Ups2 protein, S cerevisiae; C483858:phosphatidylethanolamine; D002262:Carboxy-Lyases; C577156:Psd1 protein, S cerevisiae