title: Diversity of patients microflora on orthopaedic and dental implants.
abstract: The aim of this study was to compare the diversity of microbial colonization on implant material from different individuals. Eubacterial DNA was extracted, separated and sequenced from orthopaedic metallic implant material, tissues or body fluids, and skin of 4 patients as well as from identical dental cement material from 10 individuals after revision and routine removal. Additionally, the composition of the bacterial population of the dental cement and the oral swab sample from one individual after direct extraction of bacterial DNA was compared to extraction after conventional microbiological enrichment. The latter investigation proved that the commonly used cultivation technique gave different results than direct extraction of DNA, especially as regards the detection of anaerobes. Comparing the bacterial colonization of implant materials from different patients showed significant individual diversity. The common focus on a constricted pathogen spectrum may have to be expanded toward a multispecies population. Moreover, the dependence of the bacterial population on the individual host has to be integrated in discussing implant colonization and infection.
authors.affiliation: Institute of Functional Interfaces, Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany. ursula.obst@kit.edu
authors.forename: Ursula
authors.initials: U
authors.lastname: Obst
authors.forename: Silke-Mareike
authors.initials: SM
authors.lastname: Marten
authors.forename: Christoph
authors.initials: C
authors.lastname: Niessner
authors.forename: Erich
authors.initials: E
authors.lastname: Hartwig
authors.forename: Michael
authors.initials: M
authors.lastname: Korsch
authors.forename: Winfried
authors.initials: W
authors.lastname: Walther
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D018441:Biofilms / Q000254:growth & development; D004269:DNA, Bacterial / Q000302:isolation & purification; D003738:Dental Cements / Q000009:adverse effects*; D015921:Dental Implants / Q000009:adverse effects* / Q000382:microbiology; D054884:Host-Pathogen Interactions; D006801:Humans; D019736:Prostheses and Implants / Q000009:adverse effects* / Q000382:microbiology; D016459:Prosthesis-Related Infections / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D021521:Ribotyping
chemical_list: D004269:DNA, Bacterial; D003738:Dental Cements; D015921:Dental Implants; D012336:RNA, Ribosomal, 16S