title: Enzymatic analyses demonstrate thermal adaptation of α-glucosidase activity in starch amended gully waste.
abstract: In this study we investigated the effect of starch amendment on α-glucosidase activity in an organic waste environment, treated under both mesophilic and thermophilic conditions. The relative effects of temperature on α-glucosidase activity with regard to in situ and assay conditions were investigated under a reciprocal design. The decline in dry matter under the different thermal regimes was consistent with the temperature independent degradation of starch. The results of extra-cellular enzyme analysis showed a significant relationship between starch addition and α-glucosidase activity, with evidence of thermal adaptation to the in situ temperature. A weaker, but significant, effect of starch addition on β-glucosidase activity was observed, with no evidence for thermal adaptation. Thus, our data is consistent with a substrate adaptive response to temperature, albeit under potentially a high selective pressure.
authors.affiliation: Environmental Technologies Centre for Industrial Collaboration, University of Hull, Hull HU6 7RX, UK. j.d.adams@hull.ac.uk
authors.forename: J D W
authors.initials: JD
authors.lastname: Adams
authors.forename: K M
authors.initials: KM
authors.lastname: Scott
mesh_terms: D000704:Analysis of Variance; D013213:Starch / Q000378:metabolism*; D013696:Temperature; D018505:Waste Management / Q000379:methods; D014866:Waste Products / Q000032:analysis*; D000520:alpha-Glucosidases / Q000378:metabolism*
chemical_list: D014866:Waste Products; D013213:Starch; D000520:alpha-Glucosidases