title: Molecular epidemiology of hepatitis C virus among different groups of people in the province of Fujian, China.
abstract: Genotyping of hepatitis C virus (HCV) can provide valuable information for prognosis and treatment duration prediction. To explore the genetic diversity of HCV in Fujian Province, China, 112, 104 and 48 anti-HCV-positive serum samples were collected from volunteer blood donors, IDUs and patients, respectively, from Jan 2008 to Dec 2008 and were genotyped through sequence analysis, followed by phylogenetic analysis in the C/E1 and NS5B regions. Genotypes could be determined for 85.61 and 84.85 % of samples in the C/E1 and NS5B region, respectively. 6a was the most prevalent subtype, which accounted for 42.04 and 43.75 % in the C/E1 and NS5B region, respectively. Mixed infection and potential recombination were detected in this study. Kappa tests indicated that similar results were obtained by two genotyping methods targeting the C/E1 and NS5B regions. The differences in the main prevalent subtype between the three target groups suggest diversity of HCV prevalence in different populations.
authors.affiliation: Department of Pathogenic Biology, School of Basic Medical, Fujian Medical University, No. 88, Jiaotong Road, Fuzhou, 350004 Fujian, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ye
authors.forename: Yan-Sheng
authors.initials: YS
authors.lastname: Yan
authors.forename: Ge
authors.initials: G
authors.lastname: Chen
authors.forename: Ping-Ping
authors.initials: PP
authors.lastname: Yan
authors.forename: Wu-Xiong
authors.initials: WX
authors.lastname: Zheng
authors.forename: Yan-Qin
authors.initials: YQ
authors.lastname: Deng
authors.forename: Xiu-Hui
authors.initials: XH
authors.lastname: Yang
authors.forename: Shou-Li
authors.initials: SL
authors.lastname: Wu
authors.forename: Zhi-Shan
authors.initials: ZS
authors.lastname: Zhang
mesh_terms: D001483:Base Sequence; D001782:Blood Donors; D002681:China / Q000453:epidemiology; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D016174:Hepacivirus / Q000145:classification / Q000235:genetics*; D006526:Hepatitis C / Q000453:epidemiology* / Q000821:virology*; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology; D010802:Phylogeny; D012367:RNA, Viral / Q000097:blood / Q000235:genetics; D017423:Sequence Analysis, RNA; D014759:Viral Envelope Proteins / Q000235:genetics*; D017361:Viral Nonstructural Proteins / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase
chemical_list: D012367:RNA, Viral; D014759:Viral Envelope Proteins; D017361:Viral Nonstructural Proteins; C087035:E1 protein, Hepatitis C virus; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase