title: Open fermentative production of L-lactic acid by Bacillus sp. strain NL01 using lignocellulosic hydrolyzates as low-cost raw material.
abstract: Highly efficient L-lactate production by a thermophilic strain Bacillus sp. NL01 was demonstrated in this study. Lignocellulosic hydrolyzates containing a high content of glucose, which was prepared from corn stover, was used as substrate for L-lactic acid production. The fermentation was carried out under open condition without sterilization and used NaOH as alkaline neutralizing reagent. In batch fermentation, 56.37 g l(-1) L-lactic acid was obtained from lignocellulosic hydrolyzates which contained the solid residues produced in enzymatic saccharification. In fed-batch fermentation, 75.03 g l(-1) L-lactic acid was obtained from lignocellulosic hydrolyzates supernatant. The yield was 74.5% and the average productivity was 1.04 g l(-1) h(-1).
authors.affiliation: College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China. hgouyj@njfu.edu.cn
authors.forename: Jia
authors.initials: J
authors.lastname: Ouyang
authors.forename: Rui
authors.initials: R
authors.lastname: Ma
authors.forename: Zhaojuan
authors.initials: Z
authors.lastname: Zheng
authors.forename: Cong
authors.initials: C
authors.lastname: Cai
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Ting
authors.initials: T
authors.lastname: Jiang
mesh_terms: D001407:Bacillus / Q000187:drug effects / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D003365:Costs and Cost Analysis; D005285:Fermentation; D005947:Glucose / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D019344:Lactic Acid / Q000096:biosynthesis* / Q000191:economics*; D008031:Lignin / Q000378:metabolism*; D013227:Steam; D013696:Temperature; D013997:Time Factors; D003313:Zea mays / Q000187:drug effects
chemical_list: D013227:Steam; C036909:lignocellulose; D019344:Lactic Acid; D008031:Lignin; D005947:Glucose