title: Construction of a broad-host-range Tn7-based vector for single-copy P(BAD)-controlled gene expression in gram-negative bacteria.
abstract: We describe a mini-Tn7-based broad-host-range expression cassette for arabinose-inducible gene expression from the P(BAD) promoter. This delivery vector, pTJ1, can integrate a single copy of a gene into the chromosome of Gram-negative bacteria for diverse genetic applications, of which several are discussed, using Pseudomonas aeruginosa as the model host.
authors.affiliation: Department of Microbiology, Immunology, and Cancer Biology, University of Virginia Health System, Charlottesville, Virginia, USA.
authors.forename: F Heath
authors.initials: FH
authors.lastname: Damron
authors.forename: Elizabeth S
authors.initials: ES
authors.lastname: McKenney
authors.forename: Herbert P
authors.initials: HP
authors.lastname: Schweizer
authors.forename: Joanna B
authors.initials: JB
authors.lastname: Goldberg
mesh_terms: D004251:DNA Transposable Elements; D015870:Gene Expression; D005822:Genetic Vectors; D005827:Genetics, Microbial / Q000379:methods*; D006090:Gram-Negative Bacteria / Q000235:genetics*; D058507:Host Specificity; D016254:Mutagenesis, Insertional / Q000379:methods*; D011401:Promoter Regions, Genetic
chemical_list: D004251:DNA Transposable Elements