title: Validation comparing the effectiveness of a lactic acid dip with a lactic acid spray for reducing Escherichia coli O157:H7, Salmonella, and non-O157 Shiga toxigenic Escherichia coli on beef trim and ground beef.
abstract: The objective of this research was to compare the effectiveness of two application methods (dip versus spray) of 4.4% lactic acid for reducing pathogens on inoculated beef trim and in ground beef. Beef trim inoculated with cocktail mixtures of E. coli O157:H7, non-O157 Shiga  toxigenic E. coli (STEC), or Salmonella (10(5) to 10(6) CFU/g) at separate times was subjected to five treatments: lactic acid spray (LS), lactic acid dip (LD), water spray (WS), water dip (WD), and untreated control (CTL). Intervention effectiveness for pathogen  reduction was measured at 1 and 20 h after treatment on beef trim. Trim was then ground and intervention effectiveness was measured 1 h, 24 h, 72 h, and 7 days after grinding. The LD treatment reduced all pathogens significantly (P < 0.05); E. coli O157:H7 was reduced by 0.91  to 1.41 log CFU/g on beef trim and ground beef, non-O157 STEC by 0.48 to 0.82 log CFU/g, and Salmonella by 0.51 to 0.81 log CFU/g. No other treatment significantly reduced any pathogen, although the WD treatment noticeably reduced (P > 0.05) both E. coli O157:H7 and  non-O157 STEC populations compared with the CTL. The LS treatment reduced E. coli O157:H7 and Salmonella by up to 0.5 log CFU/g on beef trim, but these reduced counts did not significantly differ (P > 0.05) from the CTL counts. Overall, the LD treatment was most effective  for reducing all pathogens and is the best of these options for improving the safety of beef trim and subsequently produced ground beef.
authors.affiliation: Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX 79409, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Wolf
authors.forename: M F
authors.initials: MF
authors.lastname: Miller
authors.forename: A R
authors.initials: AR
authors.lastname: Parks
authors.forename: G H
authors.initials: GH
authors.lastname: Loneragan
authors.forename: A J
authors.initials: AJ
authors.lastname: Garmyn
authors.forename: L D
authors.initials: LD
authors.lastname: Thompson
authors.forename: A
authors.initials: A
authors.lastname: Echeverry
authors.forename: M M
authors.initials: MM
authors.lastname: Brashears
mesh_terms: D000818:Animals; D002417:Cattle; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D004202:Disinfectants / Q000008:administration & dosage / Q000494:pharmacology*; D019453:Escherichia coli O157 / Q000187:drug effects / Q000254:growth & development; D005506:Food Contamination / Q000032:analysis / Q000517:prevention & control*; D005511:Food Handling / Q000379:methods*; D005516:Food Microbiology; D006801:Humans; D019344:Lactic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D008460:Meat / Q000382:microbiology*; D008461:Meat Products / Q000382:microbiology; D008826:Microbial Sensitivity Tests / Q000379:methods; D012475:Salmonella / Q000187:drug effects / Q000254:growth & development; D054323:Shiga-Toxigenic Escherichia coli / Q000187:drug effects / Q000254:growth & development; D013997:Time Factors
chemical_list: D004202:Disinfectants; D019344:Lactic Acid