title: Clostridium carboxidivorans strain P7T recombinant formate dehydrogenase catalyzes reduction of CO(2) to formate.
abstract: Recombinant formate dehydrogenase from the acetogen Clostridium carboxidivorans strain P7(T), expressed in Escherichia coli, shows particular activity towards NADH-dependent carbon dioxide reduction to formate due to the relative binding affinities of the substrates and products. The enzyme retains activity over 2 days at 4°C under oxic conditions.
authors.affiliation: CSIRO Biofuels Research Cluster, Research School of Chemistry, Australian National University, Canberra, Australia.
authors.forename: Apostolos
authors.initials: A
authors.lastname: Alissandratos
authors.forename: Hye-Kyung
authors.initials: HK
authors.lastname: Kim
authors.forename: Hayden
authors.initials: H
authors.lastname: Matthews
authors.forename: James E
authors.initials: JE
authors.lastname: Hennessy
authors.forename: Amy
authors.initials: A
authors.lastname: Philbrook
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Easton
mesh_terms: D002245:Carbon Dioxide / Q000378:metabolism*; D003001:Cloning, Molecular; D003013:Clostridium / Q000201:enzymology* / Q000235:genetics; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D005560:Formate Dehydrogenases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005561:Formates / Q000378:metabolism*; D015870:Gene Expression; D007700:Kinetics; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
chemical_list: D005561:Formates; D011994:Recombinant Proteins; C030544:formic acid; D002245:Carbon Dioxide; D005560:Formate Dehydrogenases