title: Ethylene inhibitors enhance shoot organogenesis of gloxinia (Sinningia speciosa).
abstract: Shoot organogenesis and plant regeneration in Sinningia speciosa were improved using ethylene inhibitors. The leaf explants were cultured on initial shoot regeneration media (MS media with BAP at 2 mg/L + NAA at 0.1 mg/L) supplemented with different concentrations of aminoethoxyvinylglycine (AVG), cobalt chloride (CoCl₂), and silver thiosulphate (STS). The addition of AVG, CoCl₂, and STS significantly improved the regeneration frequency giving higher shoots per explant and longer shoot length. The highest shoot growth was found when STS at 5 mg/L was incorporated with generation medium, performing highest regeneration frequency with highest number of shoots. This treatment (STS at 5 mg/L) produced 40% more shoots per explant compared to control followed by STS at 10 mg/L with increasing 37% more shoots compared to control. In the cases of AVG and CoCl₂ the highest shoot number per explant was found at 1 mg/L. Treated with AVG and CoCl₂ at 1 mg/L increased shoot number by 16 and 12%, respectively, compared to control. Ethylene inhibitors could be used as a possible micropropagation and plant transformation protocol in S. speciosa for plant regenerations.
authors.affiliation: Department of Horticultural Science, College of Industrial Sciences, Kongju National University, 1 Daehoe-ri, Yesan-kun, Chungnam 340-720, Republic of Korea.
authors.forename: Soo Cheon
authors.initials: SC
authors.lastname: Chae
authors.forename: Haeng Hoon
authors.initials: HH
authors.lastname: Kim
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Park
mesh_terms: D003035:Cobalt / Q000494:pharmacology; D005030:Ethylenes / Q000037:antagonists & inhibitors*; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology; D019684:Magnoliopsida / Q000187:drug effects* / Q000502:physiology*; D038081:Organogenesis / Q000187:drug effects*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018520:Plant Shoots / Q000187:drug effects* / Q000502:physiology*; D013885:Thiosulfates / Q000494:pharmacology
chemical_list: D005030:Ethylenes; D010937:Plant Growth Regulators; D013885:Thiosulfates; C095213:silver thiosulfate; D003035:Cobalt; C036216:ethylene; C018021:cobaltous chloride; C088137:aminoethoxyvinylglycine; D005998:Glycine