title: Chitosan/bovine serum albumin co-micropatterns on functionalized titanium surfaces and their effects on osteoblasts.
abstract: Chitosan (CS)/bovine serum albumin (BSA) micropatterns were prepared on functionalized Ti surfaces by micro-transfer molding (μ-TM). μ-TM realized the spatially controlled immobilization of cells and offered a new way of studying the interaction between micropatterns and cells. Two kinds of micropatterns were produced: (1) microgrooves representing a discontinuously grooved co-micropattern, with the rectangular CS region separated by BSA walls; (2) microcylinders representing a continuously interconnected co-micropattern, with the net-like CS region separated by BSA cylinders. A comparison of cell behaviors on the two types of micropatterns indicated that the shape rather than the size had a dominant effect on cell proliferation. The micropattern size in the same range of cell diameters favored cell proliferation. However, cell differentiation was more sensitive to the size rather than to the shape of the micropatterns. In conclusion, cell behavior can be regulated by micropatterns integrating different materials.
authors.affiliation: Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, Sichuan, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Xiong
authors.initials: X
authors.lastname: Lu
authors.forename: Hong
authors.initials: H
authors.lastname: Lin
authors.forename: Fuzeng
authors.initials: F
authors.lastname: Ren
authors.forename: Yang
authors.initials: Y
authors.lastname: Leng
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D008422:Materials Testing; D008954:Models, Biological; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000494:pharmacology*; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry; D014025:Titanium / Q000737:chemistry* / Q000378:metabolism
chemical_list: D020099:Coated Materials, Biocompatible; D011108:Polymers; D012710:Serum Albumin, Bovine; D048271:Chitosan; D014025:Titanium