title: Construction of an artificial pathway for isobutanol biosynthesis in the cytosol of Saccharomyces cerevisiae.
abstract: To increase isobutanol production in Saccharomyces cerevisiae, the valine biosynthetic pathway was activated by overexpression of the relevant enzymes in the mitochondria and the cytosol. Native mitochondrial enzymes were overepxressed in the cytosol by deleting the mitochondrial transit peptides. The metabolically engineered S. cerevisiae possessing the cytosolic pathway showed increased isobutanol production (63 ± 4 mg/L).
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Kobe, Japan. fmatsuda@ruby.kobe-u.ac.jp
authors.forename: Fumio
authors.initials: F
authors.lastname: Matsuda
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
authors.forename: Kengo
authors.initials: K
authors.lastname: Ida
authors.forename: Hironori
authors.initials: H
authors.lastname: Tezuka
authors.forename: Jun
authors.initials: J
authors.lastname: Ishii
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D000440:Butanols / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods*; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D000440:Butanols; C040507:isobutyl alcohol