title: Surface modification of polysulfone based hemodialysis membranes with layer by layer self assembly of polyethyleneimine/alginate-heparin: a simple polyelectrolyte blend approach for heparin immobilization.
abstract: This study intends to improve blood compatibility of polysulfone (PSF) membranes by generating a nonthrombogenic surface through heparin immobilization. To achieve this task, the support membrane prepared from a blend of PSF and sulfonated polysulfone (SPSF) was modified with layer by layer (LBL) deposition of polyethyleneimine (PEI) and alginate (ALG) and heparin blended with ALG was immobilized only on the outermost surface of the LBL assembly. The results have shown that the adsorption of human plasma proteins and platelet activation on the LBL modified membranes decreased significantly compared with the unmodified PSF and PSF-SPSF blend membranes. Furthermore, blending ALG with a small amount of heparin remarkably prolonged the APTT values of heparin free PEI/ALG coated membranes. It is envisaged that the use of a blend of HEP and ALG only in the terminating layer of the LBL assembly can be an economical and alternative modification technique to create nonthrombogenic surfaces.
authors.affiliation: Department of Chemical Engineering, Izmir Institute of Technology, Gulbahçe Koyu, 35430, Urla, Izmir, Turkey.
authors.forename: Filiz Yasar
authors.initials: FY
authors.lastname: Mahlicli
authors.forename: Sacide Alsoy
authors.initials: SA
authors.lastname: Altinkaya
mesh_terms: D000327:Adsorption; D000464:Alginates / Q000737:chemistry*; D001773:Blood Cells / Q000187:drug effects / Q000502:physiology; D002448:Cell Adhesion / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D004573:Electrolytes / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006493:Heparin / Q000737:chemistry* / Q000493:pharmacokinetics*; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D008567:Membranes, Artificial; D010973:Platelet Adhesiveness / Q000187:drug effects; D011094:Polyethyleneimine / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D006435:Renal Dialysis / Q000295:instrumentation*; D013450:Sulfones / Q000737:chemistry*; D013499:Surface Properties; D014914:Whole Blood Coagulation Time
chemical_list: D000464:Alginates; D020099:Coated Materials, Biocompatible; D004573:Electrolytes; D006603:Hexuronic Acids; D008567:Membranes, Artificial; D011108:Polymers; D013450:Sulfones; C017662:polysulfone P 1700; D020723:Glucuronic Acid; D011094:Polyethyleneimine; D006493:Heparin