title: [EGCG regulates TGF-β1-induced epithelial mesenchymal transition in squamous cell carcinoma of head and neck].
abstract: OBJECTIVE
  To study the effect and molecular mechanism of epigallocatechin-3-gallate (EGCG) on epithelial-mesenchymal transition (EMT) in vitro induced by human recombinant TGF-β1 protein in squamous cell carcinoma of the head and neck.
  
  
  METHODS
  EMT morphological changes of Tu686 cells were observed after sequential treatment of 5 ng/ml TGF-β1 and 20 µmol/L EGCG. Tu686 cells were collected after the treatment of 5 ng/ml TGF-β1 for 24 h and EGCG with different concentrations (0, 10, 20, 30 µmol/L) for another 24 h or 20 µmol/L EGCG treatment for different time phase (6, 12, 24 h). Then RT-PCR and Western-blot were applied to detect mRNA and protein expression level of epithelial cell marker E-cadherin, mesenchymal cell marker Vimentin and Smad7, an inhibit molecule of TGF-β1 mediated pathway in Tu686 cells.
  
  
  RESULTS
  TGF-β1 successfully induced characterized EMT morphological and molecular changes in Tu686 cells, in which expression of E-cadherin decreased, Vimentin increased and Smad7 declined. However, EGCG could reverse the TGF-β1 mediated process of EMT by downregulating the expression of Vimentin and upregulating the expression of E-cadherin and Smad7.
  
  
  CONCLUSIONS
  EGCG significantly inhibits TGF-β1-mediated EMT inTu686 cell lines of SCCHN, which maybe associated with the upregulated-expression of Smad7, an inhibitor in TGF-β1 signaling pathway.
authors.affiliation: Department of Otorhinolaryngology, Central South University, Changsha, China.
authors.forename: Lei-ming
authors.initials: LM
authors.lastname: Pi
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Chang-yun
authors.initials: CY
authors.lastname: Yu
authors.forename: Geng-ming
authors.initials: GM
authors.lastname: Cai
authors.forename: Dong-hai
authors.initials: DH
authors.lastname: Hunag
authors.forename: Yuan-zheng
authors.initials: YZ
authors.lastname: Qiu
authors.forename: Yong-quan
authors.initials: YQ
authors.lastname: Tian
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D045744:Cell Line, Tumor; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D006258:Head and Neck Neoplasms / Q000378:metabolism*; D006801:Humans; D015398:Signal Transduction / Q000187:drug effects; D051906:Smad7 Protein / Q000378:metabolism; D000077195:Squamous Cell Carcinoma of Head and Neck; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; C497806:SMAD7 protein, human; D051906:Smad7 Protein; D053773:Transforming Growth Factor beta1; D014746:Vimentin; D002392:Catechin; C045651:epigallocatechin gallate