title: Elevated chemokine CC-motif receptor-like 2 (CCRL2) promotes cell migration and invasion in glioblastoma.
abstract: Chemokine CC-motif receptor-like 2 (CCRL2) is a 7-transmembrane G protein-coupled receptor which plays a key role in lung dendritic cell trafficking to peripheral lymph nodes. The function and expression of CCRL2 in cancer is not understood at present. Here we report that CCRL2 expression level is elevated in human glioma patient samples and cell lines. The magnitude of increase is positively associated with increasing tumor grade, with the highest level observed in grade IV glioblastoma. By gain-of-function and loss-of-function studies, we further showed that CCRL2 did not regulate the growth of human glioblatoma U87 and U373 cells. Importantly, we demonstrated that over-expression of CCRL2 significantly enhanced the migration rate and invasiveness of the glioblastoma cells. Taken together, these results suggest for the first time that elevated CCRL2 in glioma promotes cell migration and invasion. The potential roles of CCRL2 as a novel therapeutic target and biomarker warrant further investigations.
authors.affiliation: Department of Cardiothoracic Surgery, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China.
authors.forename: Fengqiong
authors.initials: F
authors.lastname: Yin
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Xu
authors.forename: Zifeng
authors.initials: Z
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Yao
authors.forename: Zan
authors.initials: Z
authors.lastname: Shen
authors.forename: Fang
authors.initials: F
authors.lastname: Yu
authors.forename: Yiping
authors.initials: Y
authors.lastname: Tang
authors.forename: Dengli
authors.initials: D
authors.lastname: Fu
authors.forename: Sheng
authors.initials: S
authors.lastname: Lin
authors.forename: Gang
authors.initials: G
authors.lastname: Lu
authors.forename: Hsiang-Fu
authors.initials: HF
authors.lastname: Kung
authors.forename: Wai Sang
authors.initials: WS
authors.lastname: Poon
authors.forename: Yunchao
authors.initials: Y
authors.lastname: Huang
authors.forename: Marie Chia-Mi
authors.initials: MC
authors.lastname: Lin
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D016543:Central Nervous System Neoplasms / Q000378:metabolism / Q000473:pathology*; D005909:Glioblastoma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D009361:Neoplasm Invasiveness; D054388:Receptors, CCR / Q000096:biosynthesis*
chemical_list: C487531:CCRL2 protein, human; D054388:Receptors, CCR