title: Mycoplasma removal: simple curative methods for viral supernatants.
abstract: As a partner of the European Virus Archive (EVA) FP7 infrastructure, our research group is maintaining and developing a large virus collection. To meet the standards of the quality management system adopted by all European Virus Archive partners, the detection and eradication of mycoplasma in cell culture supernatants (stored at -80°C or freeze-dried) has to be improved. Although the methods for mycoplasma elimination from infected cell lines were largely described, the decontamination procedures of precious cell culture supernatants was poorly documented. In this study, a large panel of mycoplasma-contaminated virus stocks (enveloped and non enveloped, RNA and DNA viruses) was tested successfully for mycoplasma removal using two simple optimized methods. These easy-to-perform protocols, using respectively Plasmocin™ (InvivoGen, Cayla, France) and chloroform, were shown to remove mycoplasma completely from cell supernatant without incidence in viral infectivity.
authors.affiliation: Aix Marseille Univ, IRD French Institute of Research for Development, EHESP French School of Public Health, UMR_D 190 Emergence des Pathologies Virales, 13005 Marseille, France. Cecile.baronti@univ-amu.fr
authors.forename: C
authors.initials: C
authors.lastname: Baronti
authors.forename: B
authors.initials: B
authors.lastname: Pastorino
authors.forename: R
authors.initials: R
authors.lastname: Charrel
authors.forename: X
authors.initials: X
authors.lastname: de Lamballerie
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods; D002725:Chloroform / Q000494:pharmacology; D003470:Culture Media; D003666:Decontamination / Q000379:methods*; D005602:France; D018942:Macrolides / Q000494:pharmacology; D009174:Mycoplasma / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D018942:Macrolides; C554844:plasmocin; D002725:Chloroform