title: Development and validation of liquid chromatography-tandem mass spectrometry method for simultaneous determination of four tertiary alkaloids in rat plasma and its application to a pharmacokinetic study.
abstract: A specific and reliable liquid chromatography-electrospray ionization-tandem mass spectrometry method was developed for the simultaneous determination of tetrahydropalmatine (THP), tetrahydroberineper (THB), tetrahydrocoptisine (THC) and corydaline (CDL) in rat plasma using jatrorrhizine as internal standard. Plasma samples were pretreated by protein precipitation with acetonitrile. Chromatographic separation was performed on a Zorbax Eclipse Plus C18 column (3.0 mm × 100 mm, 1.8 μm) with gradient elution using acetonitrile-0.1% formic acid water as mobile phase at a flow rate of 0.3 mL/min. The detection was carried out by a triple-quadrupole tandem mass spectrometer in multiple-reaction monitoring mode. The mass transition ion-pair was followed as m/z 356.2→192.1, m/z 324.1→176.0, m/z 340.1→176.0, and m/z 370.3→192.1 for THP, THC, THB and CDL. Linear calibration curves were obtained over the range of 0.2-2000 ng/mL, and the method limits were 2.0 ng/mL for THC, 0.2 ng/mL for THB, 1.0 ng/mL for THP, and 0.4 ng/mL for CDL, respectively. The mean recovery of the analytes ranged from 85.51% to 93.99%. The intra- and inter-day precisions were in the range of 1.21-4.73% and the accuracies were between 92.23% and 98.23%. The validated method was successfully applied to a pharmacokinetic study of THP, THB, THC and CDL in rat plasma following oral administration of Jitai tablet.
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China.
authors.forename: Shuping
authors.initials: S
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Lingling
authors.initials: L
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Jiang
authors.forename: Li
authors.initials: L
authors.lastname: Xiang
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhang
authors.forename: Runhui
authors.initials: R
authors.lastname: Liu
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000470:Alkaloids / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D000818:Animals; D001599:Berberine / Q000031:analogs & derivatives / Q000737:chemistry; D001600:Berberine Alkaloids / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D002853:Chromatography, Liquid / Q000379:methods* / Q000592:standards; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards
chemical_list: D000097:Acetonitriles; D000470:Alkaloids; D001600:Berberine Alkaloids; C452799:corydaline; C055785:jatrorrhizine; D001599:Berberine; C014215:tetrahydropalmatine; C014212:stylopine; C032159:acetonitrile