title: Repairing chronic myocardial infarction with autologous mesenchymal stem cells engineered tissue in rat promotes angiogenesis and limits ventricular remodeling.
abstract: BACKGROUND
  Tissue engineering scaffold constitutes a new strategy of myocardial repair. Here, we studied the contribution of a patch using autologous mesenchymal stem cells (MSCs) seeded on collagen-1 scaffold on the cardiac reconstruction in rat model of chronic myocardial infarction (MI).
  
  
  METHODS
  Patches were cultured with controlled MSCs (growth, phenotype and potentiality). Twenty coronary ligated rats with tomoscingraphy (SPECT)-authenticated transmural chronic MI were referred into a control group (n = 10) and a treated group (n = 10) which beneficiated an epicardial MSC-patch engraftment. Contribution of MSC-patch was tested 1-mo after using non-invasive SPECT cardiac imaging, invasive hemodynamic assessment and immunohistochemistry.
  
  
  RESULTS
  3D-collagen environment affected the cell growth but not the cell phenotype and potentiality. MSC-patch integrates well the epicardial side of chronic MI scar. In treated rats, one-month SPECT data have documented an improvement of perfusion in MI segments compared to control (64 ± 4% vs 49 ± 3% p = 0.02) and a reduced infarction. Contractile parameter dp/dtmax and dp/dtmin were improved (p & 0.01). Histology showed an increase of ventricular wall thickness (1.75 ± 0.24 vs 1.35 ± 0.32 mm, p &0.05) and immunochemistry of the repaired tissue displayed enhanced angiogenesis and myofibroblast-like tissue.
  
  
  CONCLUSIONS
  3D-MSC-collagen epicardial patch engraftment contributes to reverse remodeling of chronic MI.
authors.affiliation: Department of Cardiovascular Surgery, University of Lorraine, Nancy, France. pablomaureira@hotmail.com
authors.forename: Pablo
authors.initials: P
authors.lastname: Maureira
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Marie
authors.forename: Fengxu
authors.initials: F
authors.lastname: Yu
authors.forename: Sylvain
authors.initials: S
authors.lastname: Poussier
authors.forename: Yihua
authors.initials: Y
authors.lastname: Liu
authors.forename: Frederique
authors.initials: F
authors.lastname: Groubatch
authors.forename: Aude
authors.initials: A
authors.lastname: Falanga
authors.forename: Nguyen
authors.initials: N
authors.lastname: Tran
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003094:Collagen; D004195:Disease Models, Animal; D006352:Heart Ventricles / Q000503:physiopathology; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D009203:Myocardial Infarction / Q000503:physiopathology / Q000628:therapy; D009389:Neovascularization, Pathologic; D051381:Rats; D023822:Tissue Engineering; D014182:Transplantation, Autologous; D020257:Ventricular Remodeling
chemical_list: D003094:Collagen