title: Immunogenicity of Salmonella enterica serovar Enteritidis virulence protein, InvH, and cross-reactivity of its antisera with Salmonella strains.
abstract: Acellular vaccines containing bacterial immunodominant components such as surface proteins may be potent alternatives to live attenuated vaccines in order to reduce salmonellosis risk to human health. invH gene, an important part of needle complex in type three secretion system (TTSS) plays important role in efficient bacterial adherence and entry into epithelial cells. In this work we hypothesize that use of a 15 kDa recombinant InvH as Salmonella enterica serovar Enteritidis surface protein could provoke antibody production in mouse and would help us study feasibility of its potential for diagnosis and/or a recombinant vaccine. The purified InvH provoked significant rise of IgG in mice. Active protection induced by immunization with InvH against variable doses of S. enterica serovar Enteritidis, indicated that the immunized mice were completely protected against challenge with 10(4) LD(50). The immunoreaction of sera from immunized mice with other Salmonella strains or cross reaction with sera of Salmonella strains inoculated mice is indicative of possessing by Salmonella strains of the surface protein, InvH, that can be employed in both prophylactic and diagnostic measures against S. enterica. Bacteria free spleen and ileum of the immunized mice in this study indicate that the invH gene affects bacterial invasion. Efficacy of the virulence protein, InvH, in shuttling into host cells in injectisome of S. enterica serovar Enteritidis and inhibition of this phenomenon by active immunization was shown in this study. In conclusion immunization with InvH protein can develop protection against S. enterica serovar Enteritidis infections. InvH in Salmonella strains can be exploited in protective measures as well as a diagnostic tool in Salmonella infections.
authors.affiliation: Department of Biology, Shahed University, Tehran-Qom Express Way, Opposite Imam Khomeini's Shrine, Tehran 3319118651, Iran.
authors.forename: Behzad
authors.initials: B
authors.lastname: Dehghani
authors.forename: Iraj
authors.initials: I
authors.lastname: Rasooli
authors.forename: Seyed Latif Mousavi
authors.initials: SL
authors.lastname: Gargari
authors.forename: Mohammad Reza Jalali
authors.initials: MR
authors.lastname: Nadooshan
authors.forename: Parviz
authors.initials: P
authors.lastname: Owlia
authors.forename: Shahram
authors.initials: S
authors.lastname: Nazarian
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D003429:Cross Reactions; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012475:Salmonella / Q000145:classification / Q000276:immunology* / Q000472:pathogenicity; D012480:Salmonella Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control; D012477:Salmonella enteritidis / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D007106:Immune Sera; C079729:InvH protein, Salmonella; D011994:Recombinant Proteins