title: MiR-210 disturbs mitotic progression through regulating a group of mitosis-related genes.
abstract: MiR-210 is up-regulated in multiple cancer types but its function is disputable and further investigation is necessary. Using a bioinformatics approach, we identified the putative target genes of miR-210 in hypoxia-induced CNE cells from genome-wide scale. Two functional gene groups related to cell cycle and RNA processing were recognized as the major targets of miR-210. Here, we investigated the molecular mechanism and biological consequence of miR-210 in cell cycle regulation, particularly mitosis. Hypoxia-induced up-regulation of miR-210 was highly correlated with the down-regulation of a group of mitosis-related genes, including Plk1, Cdc25B, Cyclin F, Bub1B and Fam83D. MiR-210 suppressed the expression of these genes by directly targeting their 3'-UTRs. Over-expression of exogenous miR-210 disturbed mitotic progression and caused aberrant mitosis. Furthermore, miR-210 mimic with pharmacological doses reduced tumor formation in a mouse metastatic tumor model. Taken together, these results implicate that miR-210 disturbs mitosis through targeting multi-genes involved in mitotic progression, which may contribute to its inhibitory role on tumor formation.
authors.affiliation: School of Life Sciences, Tsinghua University, Beijing 100084, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: He
authors.forename: Jiangbin
authors.initials: J
authors.lastname: Wu
authors.forename: Naihan
authors.initials: N
authors.lastname: Xu
authors.forename: Weidong
authors.initials: W
authors.lastname: Xie
authors.forename: Mengnan
authors.initials: M
authors.lastname: Li
authors.forename: Jianna
authors.initials: J
authors.lastname: Li
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Jiang
authors.forename: Burton B
authors.initials: BB
authors.lastname: Yang
authors.forename: Yaou
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology; D020090:Chromosome Segregation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000378:metabolism*; D008938:Mitosis / Q000235:genetics*; D009362:Neoplasm Metastasis
chemical_list: D020413:3' Untranslated Regions; C543800:MIRN210 microRNA, human; D035683:MicroRNAs