title: Real-time fluorescence turn-on detection of alkaline phosphatase activity with a novel perylene probe.
abstract: A tetracationic perylene probe (probe 1) was designed and synthesized. Probe 1 was used for the real-time fluorescence turn-on assay of alkaline phosphatase (ALP) activity and inhibitor screening. Probe 1 monomer fluorescence could be very efficiently quenched by ATP through the formation of an ATP/probe 1 complex. ALP triggered the degradation of ATP, the breakdown of the ATP/probe 1 complex, and the recovery of the probe 1 monomer fluorescence. In the presence of an ALP inhibitor, a decrease in fluorescence recovery was observed.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Huping
authors.initials: H
authors.lastname: Jiao
authors.forename: Wenying
authors.initials: W
authors.lastname: Li
authors.forename: Dongli
authors.initials: D
authors.lastname: Liao
authors.forename: Huipeng
authors.initials: H
authors.lastname: Zhou
authors.forename: Cong
authors.initials: C
authors.lastname: Yu
mesh_terms: D000227:Adenine Nucleotides / Q000737:chemistry; D000469:Alkaline Phosphatase / Q000378:metabolism; D005453:Fluorescence; D015335:Molecular Probes; D010569:Perylene / Q000737:chemistry*
chemical_list: D000227:Adenine Nucleotides; D015335:Molecular Probes; D010569:Perylene; D000469:Alkaline Phosphatase