title: nNOS expression in the brain of rats after burn and the effect of the ACE inhibitor captopril.
abstract: OBJECTIVE
  To investigate the role of endogenous neuronal nitric oxide synthase (nNOS) on brain injury after burn and the effects of the captopril.
  
  
  METHODS
  Wistar albino rats (200-250 g) were exposed on the dorsal surface to 90°C (burn) or 25°C (sham) water for 10 s. The ACE group was treated with intraperitoneal 10 mg/kg captopril immediately after burn and this treatment was repeated twice daily. At the end of the 24 h brain samples were taken. nNOS was studied in brain areas by immunohistochemistry.
  
  
  RESULTS
  There was no difference between the cerebellar and hypothalamic areas the nNOS expression of all groups. nNOS expression increased in the frontal cortex, striatum and midbrain in the burn group compared to the control group. In the frontal cortex, nNOS expression significantly decreased after ACE inhibitor treatment (p<0.05). The striatal nNOS of the ACE group significantly increased when compared to the control group (p=0.001). In the midbrain of the animals, nNOS decreased in the ACE group. Hippocampal nNOS expression did not change after burn and significantly increased after ACE inhibitor therapy (p<0.05).
  
  
  CONCLUSIONS
  Our data showed that the pathophysiological events following burn appear to be related to an acute inflammatory reaction which is associated with nNOS in the frontal cortex, striatum and midbrain, and captopril treatment abrogates the nNOS response in the frontal cortex and midbrain.
authors.affiliation: Baskent University, School of Medicine, Department of Pathology, 34662 Istanbul, Turkey.
authors.forename: Ebru
authors.initials: E
authors.lastname: Demiralay
authors.affiliation: Goztepe Medical Park Hospital, Medical Genetics Laboratory, Merdivenkoy, Kadikoy, Istanbul, Turkey.
authors.forename: Ibrahim Yaman
authors.initials: IY
authors.lastname: Saglam
authors.affiliation: Maltepe University, School of Medicine, Department of Pharmacology and Clinical Pharmacology, Maltepe, 34845 Istanbul, Turkey.
authors.forename: Emine Nur
authors.initials: EN
authors.lastname: Ozdamar
authors.affiliation: Marmara University, Faculty of Pharmacy, Department of Pharmacology, Haydarpasa, 34668 Istanbul, Turkey.
authors.forename: Ahmet Ozer
authors.initials: AO
authors.lastname: Sehirli
authors.affiliation: Marmara University, Faculty of Pharmacy, Department of Pharmacology, Haydarpasa, 34668 Istanbul, Turkey.
authors.forename: Goksel
authors.initials: G
authors.lastname: Sener
authors.affiliation: Uskudar University, Istanbul Neuropsychiatry Hospital, Laboratory of Clinical Pharmacogenetics, Istanbul, Turkey. Electronic address: esra.k.saglam@gmail.com.
authors.forename: Esra
authors.initials: E
authors.lastname: Saglam
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D002056:Burns / Q000188:drug therapy / Q000201:enzymology*; D002216:Captopril / Q000494:pharmacology*; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D007274:Injections, Intraperitoneal; D008297:Male; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D002216:Captopril; D052248:Nitric Oxide Synthase Type I