title: Arsenate reduction and methylation in the cells of Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1 investigated with X-ray absorption near edge structure.
abstract: Synchrotron radiation-based X-ray absorption near edge structure (XANES) was introduced to directly analysis chemical species of arsenic (As) in the cells of Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1 capable of As accumulation and volatilisation. After exposure to As(V) of 500 mg L(-1) for 15 days, a total of 60.5% and 65.3% of the accumulated As in the cells of T. asperellum SM-12F1 and P. janthinellum SM-12F4, respectively, was As(III), followed by 31.3% and 32.4% DMA (dimethylarsinic acid), 8.3% and 2.3% MMA (monomethylarsonic acid), respectively. However, for F. oxysporum CZ-8F1, 54.5% of the accumulated As was As(III), followed by 37.8% MMA and 7.7% As(V). The reduction and methylation of As(V) formed As(III), MMA, and DMA as the primacy products, and the reduction of As(V) might be more easily processed than the methylation. These results will help to understanding the mechanisms of As detoxification and its future application in bioremediation.
authors.affiliation: Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Environment, Ministry of Agriculture, Beijing, PR China. shimingsu@163.com
authors.forename: S M
authors.initials: SM
authors.lastname: Su
authors.forename: X B
authors.initials: XB
authors.lastname: Zeng
authors.forename: L F
authors.initials: LF
authors.lastname: Li
authors.forename: R
authors.initials: R
authors.lastname: Duan
authors.forename: L Y
authors.initials: LY
authors.lastname: Bai
authors.forename: A G
authors.initials: AG
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Jiang
mesh_terms: D015502:Absorptiometry, Photon; D001149:Arsenates / Q000378:metabolism*; D001151:Arsenic / Q000378:metabolism; D005670:Fusarium / Q000378:metabolism; D008745:Methylation; D010084:Oxidation-Reduction; D010407:Penicillium / Q000378:metabolism*; D012015:Reference Standards; D014242:Trichoderma / Q000378:metabolism*
chemical_list: D001149:Arsenates; D001151:Arsenic; C025657:arsenic acid