title: Loss of OPA1 disturbs cellular calcium homeostasis and sensitizes for excitotoxicity.
abstract: Optic atrophy 1 (OPA1) mutations cause dominant optic atrophy (DOA) with retinal ganglion cell (RGC) and optic nerve degeneration. The mechanism for the selective degeneration of RGCs in DOA remains elusive. To address the mechanism, we reduced OPA1 protein expression in cell lines and RGCs by RNA interference. OPA1 loss results in mitochondrial fragmentation, deficiency in oxidative phosphorylation, decreased ATP levels, decreased mitochondrial Ca(2+) retention capacity, reduced mtDNA copy numbers, and sensitization to apoptotic insults. We demonstrate profound cristae depletion and loss of crista junctions in OPA1 knockdown cells, whereas the remaining crista junctions preserve their normal size. OPA1-depleted cells exhibit decreased agonist-evoked mitochondrial Ca(2+) transients and corresponding reduction of NAD(+) to NADH, but the impairment in NADH oxidation leads to an overall more reduced mitochondrial NADH pool. Although in our model OPA1 loss in RGCs has no apparent impact on mitochondrial morphology, it decreases buffering of cytosolic Ca(2+) and sensitizes RGCs to excitotoxic injury. Exposure to glutamate triggers delayed calcium deregulation (DCD), often in a reversible manner, indicating partial resistance of RGCs to this injury. However, when OPA1 is depleted, DCD becomes irreversible. Thus, our data show that whereas OPA1 is required for mitochondrial fusion, maintenance of crista morphology and oxidative phosphorylation, loss of OPA1 also results in defective Ca(2+) homeostasis.
authors.affiliation: Apoptosis & Cell Death Program, Sanford Burnham Institute for Medical Research, La Jolla, CA 92037, USA.
authors.forename: Y E
authors.initials: YE
authors.lastname: Kushnareva
authors.forename: A A
authors.initials: AA
authors.lastname: Gerencser
authors.forename: B
authors.initials: B
authors.lastname: Bossy
authors.forename: W-K
authors.initials: WK
authors.lastname: Ju
authors.forename: A D
authors.initials: AD
authors.lastname: White
authors.forename: J
authors.initials: J
authors.lastname: Waggoner
authors.forename: M H
authors.initials: MH
authors.lastname: Ellisman
authors.forename: G
authors.initials: G
authors.lastname: Perkins
authors.forename: E
authors.initials: E
authors.lastname: Bossy-Wetzel
mesh_terms: D017209:Apoptosis; D002118:Calcium / Q000378:metabolism*; D004272:DNA, Mitochondrial / Q000378:metabolism; D020558:GTP Phosphohydrolases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009243:NAD / Q000737:chemistry / Q000378:metabolism; D029241:Optic Atrophy, Autosomal Dominant / Q000378:metabolism / Q000473:pathology; D010084:Oxidation-Reduction; D010085:Oxidative Phosphorylation; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D004272:DNA, Mitochondrial; D034741:RNA, Small Interfering; D009243:NAD; D006632:Histamine; D020558:GTP Phosphohydrolases; C415198:OPA1 protein, human; D002118:Calcium