title: Zebrafish teratogenicity testing.
abstract: As a model for teratogenicity research, zebrafish are gaining popularity and creditability. Zebrafish embryos have been proven to be a highly valuable tool in genetics and developmental biology research and have advanced our understanding of a number of known developmental toxicants. It has yet to be determined conclusively how reliably a zebrafish embryo screening assay predicts what will happen in mammalian models, but results from initial assessments have been encouraging. Here we have presented procedures for the basic care of a zebrafish colony to support embryo production, embryo collection and culturing, and teratogenicity experiments.
authors.affiliation: Charles River Laboratories, Horsham, PA, USA. Kimberly.Brannen@crl.com
authors.forename: Kimberly C
authors.initials: KC
authors.lastname: Brannen
authors.forename: Jeffrey H
authors.initials: JH
authors.lastname: Charlap
authors.forename: Elise M
authors.initials: EM
authors.lastname: Lewis
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D030801:Animals, Genetically Modified; D004625:Embryo, Nonmammalian / Q000002:abnormalities / Q000187:drug effects; D004780:Environment, Controlled; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D007267:Injections; D008297:Male; D060172:Morpholinos / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018600:Teratology / Q000379:methods*; D018675:Toxicity Tests / Q000379:methods*; D015027:Zebrafish / Q000002:abnormalities* / Q000196:embryology* / Q000235:genetics
chemical_list: D060172:Morpholinos; D012333:RNA, Messenger