title: Micro-injection of Morpholino oligonucleotides for depleting Securin in mouse oocytes.
abstract: Gene silencing techniques have brought new insights into mammalian oocyte and embryo development. More specifically, the use of Morpholino oligonucleotides which sterically inhibit translation from target mRNAs thereby compromising gene function, allowed the identification of important oocyte regulators and especially factors involved in meiotic cell cycle control. Here we describe the method of application of Morpholino oligonucleotides in mouse oocyte research.
authors.affiliation: Department of Biological Applications and Technology, University of Ioannina, Ioannina, Greece. pmaragos@cc.uoi.gr
authors.forename: Petros
authors.initials: P
authors.lastname: Marangos
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D018929:Cell Culture Techniques; D002469:Cell Separation; D005260:Female; D020868:Gene Silencing; D051379:Mice; D008845:Microinjections / Q000379:methods*; D060172:Morpholinos / Q000008:administration & dosage* / Q000235:genetics*; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D011247:Pregnancy; D064249:Securin
chemical_list: D002352:Carrier Proteins; D060172:Morpholinos; D064249:Securin