title: A new cell-selective three-dimensional microincubator based on silicon photonic crystals.
abstract: In this work, we show that vertical, high aspect-ratio (HAR) photonic crystals (PhCs), consisting of periodic arrays of 5 µm wide gaps with depth of 50 µm separated by 3 µm thick silicon walls, fabricated by electrochemical micromachining, can be used as three-dimensional microincubators, allowing cell lines to be selectively grown into the gaps. Silicon micromachined dice incorporating regions with different surface profiles, namely flat silicon and deeply etched PhC, were used as microincubators for culturing adherent cell lines with different morphology and adhesion properties. We extensively investigated and compared the proliferative behavior on HAR PhCs of eight human cell models, with different origins, such as the epithelial (SW613-B3; HeLa; SW480; HCT116; HT29) and the mesenchymal (MRC-5V1; CF; HT1080). We also verified the contribution of cell sedimentation into the silicon gaps. Fluorescence microscopy analysis highlights that only cell lines that exhibit, in the tested culture condition, the behavior typical of the mesenchymal phenotype are able to penetrate into the gaps of the PhC, extending their body deeply in the narrow gaps between adjacent silicon walls, and to grow adherent to the vertical surfaces of silicon. Results reported in this work, confirmed in various experiments, strongly support our statement that such three-dimensional microstructures have selection capabilities with regard to the cell lines that can actively populate the narrow gaps. Cells with a mesenchymal phenotype could be exploited in the next future as bioreceptors, in combination with HAR PhC optical transducers, e.g., for label-free optical detection of cellular activities involving changes in cell adhesion and/or morphology (e.g., apoptosis) in a three-dimensional microenvironment.
authors.affiliation: Dipartimento di Ingegneria Industriale e dell'Informazione, Università di Pavia, Pavia, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Carpignano
authors.forename: Gloria
authors.initials: G
authors.lastname: Silva
authors.forename: Salvatore
authors.initials: S
authors.lastname: Surdo
authors.forename: Valentina
authors.initials: V
authors.lastname: Leva
authors.forename: Alessandra
authors.initials: A
authors.lastname: Montecucco
authors.forename: Francesca
authors.initials: F
authors.lastname: Aredia
authors.forename: Anna Ivana
authors.initials: AI
authors.lastname: Scovassi
authors.forename: Sabina
authors.initials: S
authors.lastname: Merlo
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Barillaro
authors.forename: Giuliano
authors.initials: G
authors.lastname: Mazzini
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D002460:Cell Line; D003460:Crystallization; D004847:Epithelial Cells / Q000166:cytology; D006801:Humans; D008856:Microscopy, Fluorescence; D055616:Microtechnology / Q000295:instrumentation* / Q000379:methods*; D017785:Photons; D012825:Silicon / Q000737:chemistry*
chemical_list: D012825:Silicon