title: RNA silencing as a tool to uncover gene function and engineer novel traits in soybean.
abstract: RNA silencing refers collectively to diverse RNA-mediated pathways of nucleotide-sequence-specific inhibition of gene expression. It has been used to analyze gene function and engineer novel traits in various organisms. Here, we review the application of RNA silencing in soybean. To produce soybean lines, in which a particular gene is stably silenced, researchers have frequently used a transgene that transcribes inverted repeats of a target gene segment. Suppression of gene expression in developing soybean embryos has been one of the main focuses of metabolic engineering using transgene-induced silencing. Plants that have enhanced resistance against diseases caused by viruses or cyst nematode have also been produced. Meanwhile, Agrobacterium rhizogenes-mediated transformation has been used to induce RNA silencing in roots, which enabled analysis of the roles of gene products in nodulation or disease resistance. RNA silencing has also been induced using viral vectors, which is particularly useful for gene function analysis. So far, three viral vectors for virus-induced gene silencing have been developed for soybean. One of the features of the soybean genome is the presence of a large number of duplicated genes. Potential use of RNA silencing technology in combination with forward genetic approaches for analyzing duplicated genes is discussed.
authors.affiliation: Research Faculty of Agriculture, Hokkaido University , Sapporo, Hokkaido 060-8589, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Kasai
authors.forename: Akira
authors.initials: A
authors.lastname: Kanazawa
keywords: RNA interference; epigenetic changes; metabolic engineering; post-transcriptional gene silencing; soybean (Glycine max); transgene; virus-induced gene silencing