title: Gene transcription and biomarker responses in the clam Ruditapes philippinarum after exposure to ibuprofen.
abstract: Pharmaceuticals are a class of emerging environmental contaminants that continuously enter aquatic environments. Presently, little information is available about the effects of these substances on non-target organisms, such as bivalves. We investigated the effects of ibuprofen (IBU) on the clam Ruditapes philippinarum. Clams were exposed for 1, 3, 5 and 7 days to 0, 100 and 1000 μgIBU/L, and established biomarker responses (haemolymph lysozyme, gill acetylcholinesterase and digestive gland superoxide dismutase activities) as well as digestive gland transcriptome were evaluated. A two-way ANOVA revealed significant effects of both "IBU concentration" and "exposure duration" on biomarker responses. Overall, the enzyme activities were generally lower in IBU-exposed clams than in controls. Although limited knowledge of the mollusc transcriptome makes it difficult to interpret the effects of IBU on clams, the gene transcription analysis using DNA microarrays enabled the identification of the putative molecular mode of action of the IBU. The functional analysis of differentially transcribed genes suggests that IBU can interfere with various signalling pathways in clams, such as arachidonic acid metabolism, apoptosis, peroxisomal proliferator-activated receptors, and nuclear factor-kappa B. In addition, several genes involved in the metabolism of xenobiotics (e.g., glutathione S-transferase, sulfotransferase, cytochrome P450) were also found to be significantly affected by IBU exposure. In summary, the integrated approach of gene transcription analysis and biomarker responses facilitated the elucidation of the putative mechanisms of action of IBU in non-target species.
authors.affiliation: Department of Comparative Biomedicine and Food Science, University of Padova, Legnaro, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Milan
authors.forename: Marianna
authors.initials: M
authors.lastname: Pauletto
authors.forename: Tomaso
authors.initials: T
authors.lastname: Patarnello
authors.forename: Luca
authors.initials: L
authors.lastname: Bargelloni
authors.forename: Maria Gabriella
authors.initials: MG
authors.lastname: Marin
authors.forename: Valerio
authors.initials: V
authors.lastname: Matozzo
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D049872:Bivalvia / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D005880:Gills / Q000187:drug effects; D007052:Ibuprofen / Q000633:toxicity*; D059467:Transcriptome; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical; D007052:Ibuprofen