title: Weight loss resistance: a further consideration for the nutritional management of obese Equidae.
abstract: Evidence-based, weight loss management advice is required to address equine obesity. Changes in body mass (BM), body condition score (BCS), heart (HG) and belly circumference (BG), direct (ultrasonographic) and indirect (D(2)O dilution, bioelectrical impedance analysis [BIA]) measures of body fat as well as indices of insulin resistance (IR) were monitored in 12 overweight (BCS ≥ 7/9) horses and ponies of mixed breed and gender for 16 weeks. Animals were randomly assigned to two groups (Group 1, n=6, BCS 7.6/9 ± 0.6, 489 ± 184.6 kg; Group 2, n=6, BCS 8.1/9 ± 0.6, 479 ± 191.5 kg). Daily dry matter intake (DMI) was restricted to 1.25% BM as one of two, near-isocaloric (DE ∼0.115 MJ/kg BM/day), forage-based diets (Group 1, 0.8% BM chaff-based feed: 0.45% BM hay; Group 2, 1.15% BM hay: 0.1% BM nutrient-balancer). Statistical modelling revealed considerable between-animal heterogeneity in proportional weight losses (0.16-0.55% of Week 1 BM weekly). The magnitude of weight loss resistance (WLR) or sensitivity to dietary restriction was independent of diet or any measured outset variable and was largely (65%) attributed to animal identity. Predicted rates of weight loss decreased over time. BCS and BIA were poor estimates of D(2)O-derived body fat%. Reciprocal changes in depths of retroperitoneal and subcutaneous adipose tissues were evident. Changes in BG were associated with losses in retroperitoneal fat and BM (r(2), 0.67 and 0.79). Indices of IR improved for 9/12 animals by Week 16. For obese animals, weight loss should be initiated by restricting forage DMI to 1.25% BM. Subsequent restriction to 1% BM may be warranted for WLR animals.
authors.affiliation: University of Liverpool, Department of Obesity and Endocrinology, Faculty of Health and Life Sciences, Leahurst Campus, Chester High Road, Neston, Wirral CH64 7TE, UK. c.m.argo@liverpool.ac.uk
authors.forename: Caroline McG
authors.initials: CM
authors.lastname: Argo
authors.forename: Gemma C
authors.initials: GC
authors.lastname: Curtis
authors.forename: Dai
authors.initials: D
authors.lastname: Grove-White
authors.forename: Alexandra H A
authors.initials: AH
authors.lastname: Dugdale
authors.forename: Clare F
authors.initials: CF
authors.lastname: Barfoot
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Harris
mesh_terms: D000273:Adipose Tissue; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001823:Body Composition; D049628:Body Size; D004032:Diet / Q000662:veterinary; D005260:Female; D006734:Horse Diseases / Q000178:diet therapy* / Q000503:physiopathology; D006736:Horses; D007333:Insulin Resistance; D050152:Intra-Abdominal Fat / Q000503:physiopathology; D008297:Male; D009765:Obesity / Q000178:diet therapy / Q000503:physiopathology / Q000662:veterinary*; D050177:Overweight / Q000178:diet therapy / Q000662:veterinary*; D050151:Subcutaneous Fat / Q000503:physiopathology; D015431:Weight Loss