title: Purification and characterization of an antioxidant glycoprotein from the hydrolysate of Mustelus griseus.
abstract: An antioxidant glycoprotein (Fraction AIV-2) with molecular weight of 27.2 kDa was purified from the ethanol-soluble protein hydrolysate of Mustelus griseus muscle. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of Fraction AIV-2 reached up to 96.73±2.33% and was higher than that of ascorbic acid at the concentration of 5.0mg/mL. Total protein and carbohydrate contents of Fraction AIV-2 were 62.65±0.63% and 33.49±1.60%, respectively. Seventeen amino acids were identified in Fraction AIV-2, most of which are serine. GC-MS analysis showed that Fraction AIV-2 was composed of fucose, arabinose, galactose, glucose and mannose with the ratio of 1.00:1.53:7.27:9.07:2.09. The FT-IR spectrum of Fraction AIV-2 showed typical characteristics of polysaccharide and protein. For Fraction AIV-2, the changes of ultraviolet absorption curve, amino acid composition after the β-elimination reaction and its deglycosylation with the treatment of N-glycosidase F suggested that both O-glycosidic and N-glycosidic bonds were involved in the polysaccharide and protein moieties.
authors.affiliation: School of Food and Pharmacy, Zhejiang Ocean University, Qixiangtai Road 51, Zhoushan 316004, PR China.
authors.forename: Zhongrui
authors.initials: Z
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Changfeng
authors.initials: C
authors.lastname: Chi
authors.forename: Yandan
authors.initials: Y
authors.lastname: Gong
authors.forename: Jiajia
authors.initials: J
authors.lastname: Tang
authors.forename: Hongyu
authors.initials: H
authors.lastname: Luo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification; D029941:Fish Proteins / Q000737:chemistry / Q000302:isolation & purification; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification; D009124:Muscle Proteins / Q000737:chemistry / Q000302:isolation & purification; D012754:Sharks
chemical_list: D000975:Antioxidants; D029941:Fish Proteins; D006023:Glycoproteins; D009124:Muscle Proteins