title: Oxidative burst inhibitory and cytotoxic activity of constituents of the fruits of Odyendyea gabonensis.
abstract: The methanol extract of dried fruits of Odyendyea gabonensis afforded one new quassinoid [(-)-odyendanol (1)], one new canthin-6-one alkaloid [9-hydroxy-5-methoxycanthin-6-one (4)], and two new steroids [22E, 24R-stigmasta-5,22-diene-3,7-dione (7) and 22E,24R-stigmast-22-ene-3,7-dione (8)] along with fourteen known compounds. The structures of all compounds were established by analyzing the spectroscopic data. The ¹³C-NMR values of (-)-odyendene (2) and (-)-odyendane (3), as well as the single-crystal X-ray structure of 5-methoxycanthin-6-one (6) are also reported.The oxidative burst inhibitory activity of pure compounds 1-12 was determined by the chemoluminescence assay, and cytotoxic activities of compounds 2-6 against the human prostate cancer cell PC-3 line were evaluated. Compounds 1-6 exhibited a clear suppressive effect on the phagocytosis response upon activation with serum-opsonized zymosan in the range of IC₅₀ = 0.9-2.0 µM versus ibuprofen with IC₅₀ = 12.1 µM, while all canthin-6-one alkaloids (4-6) displayed moderate cytotoxic activity against the human prostate cancer cell PC-3 line, with IC₅₀ values ranging from 13.5-15.4 µM versus doxorubicine with IC₅₀ = 1.5 µM.
authors.affiliation: Department of Chemistry, Faculty of Sciences, University of Douala, Douala, Cameroon.
authors.forename: Suzye Mireille
authors.initials: SM
authors.lastname: Moladje Donkwe
authors.forename: Emmanuel Ngeufa
authors.initials: EN
authors.lastname: Happi
authors.forename: Jean Duplex
authors.initials: JD
authors.lastname: Wansi
authors.forename: Bruno
authors.initials: B
authors.lastname: Ndjakou Lenta
authors.forename: Krishna Prasad
authors.initials: KP
authors.lastname: Devkota
authors.forename: Beate
authors.initials: B
authors.lastname: Neumann
authors.forename: Hans-Georg
authors.initials: HG
authors.lastname: Stammler
authors.forename: Norbert
authors.initials: N
authors.lastname: Sewald
mesh_terms: D000470:Alkaloids / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D002243:Carbolines / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry; D006801:Humans; D026121:Indole Alkaloids / Q000494:pharmacology; D008297:Male; D015394:Molecular Structure; D010840:Phytosterols / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D036702:Quassins / Q000494:pharmacology; D016897:Respiratory Burst / Q000187:drug effects*; D029641:Simaroubaceae / Q000737:chemistry*; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D002243:Carbolines; D026121:Indole Alkaloids; D010840:Phytosterols; D010936:Plant Extracts; D036702:Quassins; C453379:canthin-6-one