title: Protective effects of Shichimotsu-koka-To on irreversible Thy-1 nephritis.
abstract: Oxidative stress and peritubular capillary (PTC) injury are involved in the progression of chronic kidney disease (CKD). We investigated protective effects of Shichimotsu-koka-To (SKT), a Japanese traditional Kampo prescription, against nephrosclerosis and hypertension on a CKD model due to irreversible nephritis. Six-week-old male Wistar rats were subjected to uninephrectomy, and to injection of rabbit anti-thymocyte serum. SKT treatment was continued for 15 weeks, blood pressure was measured, and then renal specimens were collected. PTC networks were detected by immunostaining for CD-31. And superoxide dismutase (SOD)-like activity in the tissue was evaluated. Blood pressure in the SKT group, as well as sham group, was significantly lower than with the vehicle. SKT markedly ameliorated renal function, which was evaluated with urea nitrogen clearance. Compared with the vehicle, SKT treatment lowered both the glomerular enlargement and hyper-cellularity by 80%, and decreased the extracellular matrix area by 75%. SKT treatment also suppressed tubular injury, and maintained PTC networks. Furthermore, SKT recovered SOD-like activity to the basal levels. These results suggest that SKT may be useful for the treatment of CKD during the progression to nephrosclerosis, through the mechanisms of anti-oxidative activity and maintenance of PTC networks.
authors.affiliation: Division of Nephrology, Department of Internal Medicine, Atami Hospital, Intenational University of Health and Welfare, 13–1 Higashikaigan-cho, Atami 413–0012, Japan.
authors.forename: Takahiko
authors.initials: T
authors.lastname: Ono
authors.forename: Kohei
authors.initials: K
authors.lastname: Kamikado
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Morimoto
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001806:Blood Urea Nitrogen; D024141:Collagen Type IV / Q000378:metabolism; D015432:Glomerulonephritis, Membranoproliferative / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008297:Male; D020835:Medicine, Kampo; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D024141:Collagen Type IV; D010936:Plant Extracts; D020011:Protective Agents; C116087:shichimotsu-koka-to; D013482:Superoxide Dismutase