title: Efficacy of ivermectin versus dual infections of Haemonchus contortus and Heligmosomoides polygyrus in the mouse.
abstract: This report describes a novel assay for the detection of gastrointestinal anthelmintics using mice infected with Haemonchus contortus and adapted to the 1 animal/test group protocol. Mice infected with both H. contortus and Heligmosomoides polygyrus were fed ivermectin-medicated diets for 6 days. A dietary level of 0.09375 ppm was 98.1% effective against the 0- to 6-day-old abomasal stomach worm of sheep, whereas a level of 0.75 ppm reduced the 3- to 9-day-old H. polygyrus worm burden by 94.0%. H. contortus was approximately 8-fold more sensitive to ivermectin than was H. polygyrus in this model. The sensitivity of this assay rivals that of the gerbil-Trichostrongylus colubriformis model while utilizing a more economical host.
authors.affiliation: Merck Research Laboratories, P.O. Box 2000, Rahway, New Jersey 07065, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Ostlind
authors.forename: W G
authors.initials: WG
authors.lastname: Mickle
authors.forename: S
authors.initials: S
authors.lastname: Smith
authors.forename: D V
authors.initials: DV
authors.lastname: Ewanciw
authors.forename: S
authors.initials: S
authors.lastname: Cifelli
mesh_terms: D000821:Animal Feed; D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005260:Female; D006188:Haemonchiasis / Q000188:drug therapy*; D006189:Haemonchus / Q000187:drug effects; D007559:Ivermectin / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D051379:Mice; D009351:Nematospiroides dubius / Q000187:drug effects*; D012680:Sensitivity and Specificity; D017206:Strongylida Infections / Q000188:drug therapy*
chemical_list: D000871:Anthelmintics; D007559:Ivermectin