title: Use of real-time quantitative PCR to document successful treatment of Mycoplasma haemocanis infection with doxycycline in a dog.
abstract: An 8-year-old Jack Russell Terrier was diagnosed with hemolytic anemia caused by hemoplasmosis 4 years following splenectomy. Quantitative real-time PCR (qPCR) analysis was used initially to confirm infection with Mycoplasma haemocanis and subsequently to monitor and direct medical therapy. Doxycycline was administered beyond resolution of clinical signs until hemoplasma DNA could no longer be detected by qPCR. The dog remained clinically healthy and hemoplasma-negative 8 months following cessation of therapy. Canine hemoplasmosis should remain as a differential diagnosis for hemolytic anemia in dogs, particularly those that are splenectomized or immunocompromised, even in geographic regions where prevalence of infection is low. Prolonged doxycycline administration has been shown by qPCR to lead to sustained absence of detectable infection and should be considered as a first line treatment for canine hemoplasmosis.
authors.affiliation: Carmichael Torrance Diagnostic Services, Garforth, UK. pitorrifra@gmail.com
authors.forename: Francesca
authors.initials: F
authors.lastname: Pitorri
authors.forename: Marta
authors.initials: M
authors.lastname: Dell'Orco
authors.forename: Nick
authors.initials: N
authors.lastname: Carmichael
authors.forename: Emily N
authors.initials: EN
authors.lastname: Barker
authors.forename: Mike
authors.initials: M
authors.lastname: Hollywood
authors.forename: Séverine
authors.initials: S
authors.lastname: Tasker
mesh_terms: D000743:Anemia, Hemolytic / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D004269:DNA, Bacterial / Q000235:genetics; D003937:Diagnosis, Differential; D004283:Dog Diseases / Q000188:drug therapy* / Q000469:parasitology; D004285:Dogs; D004318:Doxycycline / Q000008:administration & dosage*; D004912:Erythrocytes / Q000166:cytology / Q000469:parasitology; D008297:Male; D009174:Mycoplasma / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary*; D013156:Splenectomy / Q000662:veterinary; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004318:Doxycycline