title: Black soybean anthocyanins inhibit adipocyte differentiation in 3T3-L1 cells.
abstract: Anthocyanins are naturally occurring polyphenolic pigments in plants that have been shown to decrease weight gain and insulin resistance in mice-fed high-fat diets. We investigated the effects of anthocyanins on cell growth, differentiation, and lipolysis in 3T3-L1 cells to test our hypothesis that anthocyanins could reduce adipose tissue mass by acting directly on adipocytes. Anthocyanin extracts from black soybeans were used and composed of 3 of the following major anthocyanins: cyanidine-3-O-glucoside (68.3%), delphinidin-3-O-glucoside (25.2%), and petunidin-3-O-glucoside (6.5%). Treatment with 12.5 and 50 μg/mL of black soybean anthocyanins exhibited inhibitory effects on the proliferation of both preconfluent preadipocytes (P < .01) and maturing postconfluent adipocytes (P < .01). In fully differentiated adipocytes, the number of viable cells was reduced by black soybean anthocyanins (P < .01). Treatment with 50 μg/mL of black soybean anthocyanins slightly increased epinephrine-induced lipolysis but decreased the basal lipolysis of fully differentiated adipocytes (P < .05). Black soybean anthocyanins also reduced lipid accumulation and suppressed the expression of the peroxisome proliferator-activated receptor γ, a major transcription factor for the adipogenic gene (P < .01). These results suggest that black soybean anthocyanins inhibit adipocyte differentiation and basal lipolysis, which may contribute to their antiobesity and antidiabetic properties.
authors.affiliation: Department of Food Science and Nutrition, The Catholic University of Korea, Bucheon, Republic of Korea. hkyeong@catholic.ac.kr
authors.forename: Hye-Kyeong
authors.initials: HK
authors.lastname: Kim
authors.forename: Jin Nam
authors.initials: JN
authors.lastname: Kim
authors.forename: Sung Nim
authors.initials: SN
authors.lastname: Han
authors.forename: Jae-Hwan
authors.initials: JH
authors.lastname: Nam
authors.forename: Ha-Na
authors.initials: HN
authors.lastname: Na
authors.forename: Tae Joung
authors.initials: TJ
authors.lastname: Ha
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects*; D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D000872:Anthocyanins / Q000494:pharmacology* / Q000627:therapeutic use; D019440:Anti-Obesity Agents / Q000494:pharmacology* / Q000627:therapeutic use; D049109:Cell Proliferation / Q000187:drug effects; D004837:Epinephrine / Q000494:pharmacology; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use; D050356:Lipid Metabolism / Q000187:drug effects*; D008066:Lipolysis / Q000187:drug effects; D051379:Mice; D009765:Obesity / Q000378:metabolism / Q000517:prevention & control; D047495:PPAR gamma / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D012639:Seeds / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry*
chemical_list: D000872:Anthocyanins; D019440:Anti-Obesity Agents; D007004:Hypoglycemic Agents; D047495:PPAR gamma; D010936:Plant Extracts; D004837:Epinephrine