title: Construction and characterisation of an antifungal recombinant Bacillus thuringiensis with an expanded host spectrum.
abstract: A novel antifungal Bacillus thuringiensis strain 19-22, ssp. kurstaki (H3a3b3c), was characterised. This strain included cry1Aa, cry1Ab, cry1Ac, and cry1D, which have high insecticidal activities against lepidopteran larvae other than Spodoptera exigua. To expand the host spectrum, a cry1E gene whose product is active against S. exigua was introduced into the isolate. The transformant successfully expressed the Cry1E protein without any loss of its original antifungal activities. These results indicate that this recombinant strain exhibits dual activities and may be used as an integrated control agent to control plant diseases and insect pests.
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Science, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Qin
authors.initials: Q
authors.lastname: Liu
authors.forename: Jong Yul
authors.initials: JY
authors.lastname: Roh
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Choi
authors.forename: Xue Ying
authors.initials: XY
authors.lastname: Tao
authors.forename: Jae Su
authors.initials: JS
authors.lastname: Kim
authors.forename: Yeon Ho
authors.initials: YH
authors.lastname: Je
mesh_terms: D000818:Animals; D000898:Antibiosis; D001413:Bacillus thuringiensis / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D005658:Fungi / Q000502:physiology*; D005818:Genetic Engineering; D058507:Host Specificity; D010571:Pest Control; D010935:Plant Diseases / Q000382:microbiology* / Q000469:parasitology*; D012988:Soil Microbiology; D018411:Spodoptera / Q000502:physiology*