title: Highly efficient extraction of cellular nucleic acid associated proteins in vitro with magnetic oxidized carbon nanotubes.
abstract: Nucleic acid associated proteins (NAaP) play the essential roles in gene regulation and protein expression. The global analysis of cellular NAaP would give a broad insight to understand the interaction between nucleic acids and the associated proteins, such as the important proteinous regulation factors on nucleic acids. Proteomic analysis presents a novel strategy to investigate a group of proteins. However, the large scale analysis of NAaP is yet impossible due to the lack of approaches to harvest target protein groups with a high efficiency. Herein, a simple and efficient method was developed to collect cellular NAaP using magnetic oxidized carbon nanotubes based on the strong interaction between carbon nanotubes and nucleic acids along with corresponding associated proteins. We found that the magnetic oxidized carbon nanotubes demonstrated a nearly 100% extraction efficiency for intracellular nucleic acids from cells in vitro. Importantly, the proteins associated on nucleic acids could be highly efficiently harvested using magnetic oxidized carbon nanotubes due to the binding of NAaP on nucleic acids. 1594 groups of nuclear NAaP and 2595 groups of cellular NAaP were extracted and identified from about 1,000,000 cells, and 803 groups of NAaP were analyzed with only about 10,000 cells, showing a promising performance for the proteomic analysis of NAaP from minute cellular samples. This highly efficient extraction strategy for NAaP is a simple approach to identify cellular nucleic acid associated proteome, and we believed this strategy could be further applied in systems biology to understand the gene expression and regulation.
authors.affiliation: CAS Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhengyan
authors.initials: Z
authors.lastname: Hu
authors.forename: Hongqiang
authors.initials: H
authors.lastname: Qin
authors.forename: Xiaoluan
authors.initials: X
authors.lastname: Wei
authors.forename: Kai
authors.initials: K
authors.lastname: Cheng
authors.forename: Fangjie
authors.initials: F
authors.lastname: Liu
authors.forename: Ren'an
authors.initials: R
authors.lastname: Wu
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002853:Chromatography, Liquid; D004268:DNA-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism; D005290:Ferric Compounds / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*; D008280:Magnetics; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009696:Nucleic Acids / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D053719:Tandem Mass Spectrometry; D014407:Tumor Cells, Cultured
chemical_list: D004268:DNA-Binding Proteins; D005290:Ferric Compounds; D037742:Nanotubes, Carbon; D009696:Nucleic Acids; D020543:Proteome; C000499:ferric oxide