title: Genomic sequence of an infectious bursal disease virus isolate from Zambia: classical attenuated segment B reassortment in nature with existing very virulent segment A.
abstract: We determined the complete nucleotide sequence of an infectious bursal disease (IBD) virus (IBDV) isolate (designated KZC-104) from a confirmed IBD outbreak in Lusaka in 2004. The genome consisted of 3,074 and 2,651 nucleotides in the coding regions of segments A and B, respectively. Alignment of both nucleotide and deduced amino acid sequences and phylogenetic analysis revealed that the genome segment A of KZC-104 was derived from a very virulent (VV) strain, whereas its segment B was derived from a classical attenuated strain. On BLAST search, the full-length segment A and B sequences showed 98 % nucleotide sequence identity to the VV strain D6948 and 99.8 % nucleotide sequence identity to the classical attenuated strain D78. This is a unique IBDV reassortant strain that has emerged in nature, involving segment B of a cell-culture-adapted attenuated vaccine.
authors.affiliation: Department of Veterinary Microbiology and Parasitology, Molecular Biology and Biotechnology Section, Sokoine University of Agriculture, P. O. Box 3019, Chuo kikuu, Morogoro, Tanzania. chrisskasa@gmail.com
authors.forename: C J
authors.initials: CJ
authors.lastname: Kasanga
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: H M
authors.initials: HM
authors.lastname: Munang'andu
authors.forename: K
authors.initials: K
authors.lastname: Ohya
authors.forename: H
authors.initials: H
authors.lastname: Fukushi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018175:Birnaviridae Infections / Q000662:veterinary* / Q000821:virology; D002645:Chickens; D004196:Disease Outbreaks / Q000662:veterinary; D016679:Genome, Viral; D007243:Infectious bursal disease virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology*; D016865:Reassortant Viruses / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics; D015024:Zambia
chemical_list: C107669:VP1 protein, infectious bursal disease virus; C518635:VP5 protein, infectious bursal disease virus; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins