title: Oxidative stress gene expression profile in inbred mouse after ischemia/reperfusion small bowel injury.
abstract: OBJECTIVE
  To determine the profile of gene expressions associated with oxidative stress and thereby contribute to establish parameters about the role of enzyme clusters related to the ischemia/reperfusion intestinal injury.
  
  
  METHODS
  Twelve male inbred mice (C57BL/6) were randomly assigned: Control Group (CG) submitted to anesthesia, laparotomy and observed by 120 min; Ischemia/reperfusion Group (IRG) submitted to anesthesia, laparotomy, 60 min of small bowel ischemia and 60 min of reperfusion. A pool of six samples was submitted to the qPCR-RT protocol (six clusters) for mouse oxidative stress and antioxidant defense pathways.
  
  
  RESULTS
  On the 84 genes investigated, 64 (76.2%) had statistic significant expression and 20 (23.8%) showed no statistical difference to the control group. From these 64 significantly expressed genes, 60 (93.7%) were up-regulated and 04 (6.3%) were down-regulated. From the group with no statistical significantly expression, 12 genes were up-regulated and 8 genes were down-regulated. Surprisingly, 37 (44.04%) showed a higher than threefold up-regulation and then arbitrarily the values was considered as a very significant. Thus, 37 genes (44.04%) were expressed very significantly up-regulated. The remained 47 (55.9%) genes were up-regulated less than three folds (35 genes - 41.6%) or down-regulated less than three folds (12 genes - 14.3%).
  
  
  CONCLUSIONS
  The intestinal ischemia and reperfusion promote a global hyper-expression profile of six different clusters genes related to antioxidant defense and oxidative stress.
authors.affiliation: Medical School, UFGD, Dourados, MS, Brazil. bertoletto@terra.com.br
authors.forename: Paulo Roberto
authors.initials: PR
authors.lastname: Bertoletto
authors.forename: Adauto Tsutomu
authors.initials: AT
authors.lastname: Ikejiri
authors.forename: Frederico
authors.initials: F
authors.lastname: Somaio Neto
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Chaves
authors.forename: Roberto
authors.initials: R
authors.lastname: Teruya
authors.forename: Eduardo Rodrigues
authors.initials: ER
authors.lastname: Bertoletto
authors.forename: Murched Omar
authors.initials: MO
authors.lastname: Taha
authors.forename: Djalma José
authors.initials: DJ
authors.lastname: Fagundes
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015536:Down-Regulation / Q000235:genetics; D020869:Gene Expression Profiling; D007421:Intestine, Small / Q000098:blood supply* / Q000601:surgery*; D007511:Ischemia / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D005810:Multigene Family / Q000235:genetics; D018384:Oxidative Stress / Q000235:genetics*; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction; D015427:Reperfusion Injury / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D015854:Up-Regulation / Q000235:genetics
chemical_list: D000975:Antioxidants