title: Discovery of high-confidence single nucleotide polymorphisms from large-scale de novo analysis of leaf transcripts of Aegilops tauschii, a wild wheat progenitor.
abstract: Construction of high-resolution genetic maps is important for genetic and genomic research, as well as for molecular breeding. Single nucleotide polymorphisms (SNPs) are the predominant class of genetic variation and can be used as molecular markers. Aegilops tauschii, the D-genome donor of common wheat, is considered a valuable genetic resource for wheat improvement. Our previous study implied that Ae. tauschii accessions can be genealogically divided into two major lineages. In this study, the transcriptome of two Ae. tauschii accessions from each lineage, lineage 1 (L1) and 2 (L2), was sequenced, yielding 9435 SNPs and 739 insertion/deletion polymorphisms (indels) after de novo assembly of the reads. Based on 36 contig sequences, 31 SNPs and six indels were validated on 20 diverse Ae. tauschii accessions. Because almost all of the SNP markers were polymorphic between L1 and L2, and the D-genome donor of common wheat is presumed to belong to L2, these markers are available for D-genome typing in crosses between common wheat varieties and L1-derived synthetic wheat. Due to the conserved synteny between wheat and barley chromosomes, the high-density expressed sequence tag barley map and the hypothetical gene order in barley can be applied to develop markers on target chromosomal regions in wheat.
authors.affiliation: Laboratory of Plant Genetics, Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Julio Cesar Masaru
authors.initials: JC
authors.lastname: Iehisa
authors.forename: Akifumi
authors.initials: A
authors.lastname: Shimizu
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Sato
authors.forename: Shuhei
authors.initials: S
authors.lastname: Nasuda
authors.forename: Shigeo
authors.initials: S
authors.lastname: Takumi
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics*; D020451:Contig Mapping; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D020224:Expressed Sequence Tags; D015723:Gene Library; D008040:Genetic Linkage; D018745:Genome, Plant / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D054643:INDEL Mutation; D018895:Microsatellite Repeats / Q000235:genetics; D058977:Molecular Sequence Annotation; D059372:Nucleotide Motifs; D018515:Plant Leaves / Q000235:genetics; D006109:Poaceae / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics; D017422:Sequence Analysis, DNA; D026801:Synteny; D059467:Transcriptome
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D018749:RNA, Plant