title: Epigenetic profile in chronic lymphocytic leukemia using methylation-specific multiplex ligation-dependent probe amplification.
abstract: OBJECTIVE
  To analyze the methylation status of 35 tumor suppressor genes using methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) in chronic lymphocytic leukemia (CLL).
  
  
  METHODS
  The DNA of 37 samples from patients with CLL, six healthy donors, and Jurkat and Ramos cell lines was analyzed by MS-MLPA.
  
  
  RESULTS
  Our results confirm that hypermethylation is a common and not randomly distributed event in CLL, and some genes, such as WT1, CDH13, IGSF4/TSLC1, GATA5, DAPK1 and RARB, are hypermethylated in more than 25% of the analyzed samples. Importantly, MS-MLPA also detected hypermethylation of some genes not reported previously in CLL, and their methylation status was confirmed by bisulfite sequencing.
  
  
  CONCLUSIONS
  These results indicate that MS-MLPA is a useful technique for the detection of methylation in CLL samples. Selecting CLL-specific methylation targets in order to generate a CLL-specific MS-MLPA probe set could enhance its usefulness as a tool in studies of risk stratification and guiding the best therapeutic decision.
authors.affiliation: Departament de Ciències Fisiològiques II, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)-Universitat de Barcelona, L'Hospitalet de Llobregat, Barcelona, Spain.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Cosialls
authors.forename: Antonio F
authors.initials: AF
authors.lastname: Santidrián
authors.forename: Llorenç
authors.initials: L
authors.lastname: Coll-Mulet
authors.forename: Daniel
authors.initials: D
authors.lastname: Iglesias-Serret
authors.forename: Diana M
authors.initials: DM
authors.lastname: González-Gironès
authors.forename: Alba
authors.initials: A
authors.lastname: Pérez-Perarnau
authors.forename: Camila
authors.initials: C
authors.lastname: Rubio-Patiño
authors.forename: Eva
authors.initials: E
authors.lastname: González-Barca
authors.forename: Esther
authors.initials: E
authors.lastname: Alonso
authors.forename: Gabriel
authors.initials: G
authors.lastname: Pons
authors.forename: Joan
authors.initials: J
authors.lastname: Gil
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015820:Cadherins / Q000235:genetics; D016022:Case-Control Studies; D018899:CpG Islands; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005260:Female; D016147:Genes, Tumor Suppressor; D016162:Genes, Wilms Tumor; D014644:Genetic Variation; D006801:Humans; D019169:Jurkat Cells; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D011401:Promoter Regions, Genetic; D018168:Receptors, Retinoic Acid / Q000235:genetics; D015203:Reproducibility of Results
chemical_list: D015820:Cadherins; C099972:H-cadherin; D018168:Receptors, Retinoic Acid; C082925:retinoic acid receptor beta