title: Schistosoma mansoni tetraspanning orphan receptor (SmTOR): a new vaccine candidate against schistosomiasis.
abstract: One approach to fight against schistosomiasis is to develop an efficient vaccine. Schistosoma mansoni tetraspanning orphan receptor (SmTOR) might be a vaccine candidate, as it is a tegument membrane protein expressed most highly in cercariae. In this study we characterized the recombinant first extracellular domain of SmTOR (rSmTORed1) as having the expected property to bind C2 of complement similarly to a smaller peptide of the same domain, and to produce specific and high-titre antibodies in BALB/c mice immunized using complete Freund's adjuvant/incomplete Freund's adjuvant (CFA/IFA). Immunization was protective against parasite infection, as demonstrated by a significant decrease in worm burden in immunized BALB/c mice versus the control groups over two independent trials [64 and 45% reduction for mean adult worm burden in immunized versus phosphate-bufferd saline (PBS) injected mice]. Interestingly, infection by itself did not lead to the generation of anti-rSmTORed1 antibodies, corresponding to the low frequency of specific anti-rSmTORed1 antibodies detected in the sera of patients infected with S. mansoni (2/20; 10%). These data suggest that, as opposed to the natural infection during which SmTOR induces antibodies only rarely, immunization with its smaller first extracellular domain might be more efficient.
authors.affiliation: Immunonephrology Laboratory, Department of Biomedicine, University Hospital Basel, Basel, Switzerland. c.lochmatter@me.com
authors.forename: C
authors.initials: C
authors.lastname: Lochmatter
authors.forename: C L
authors.initials: CL
authors.lastname: Schneider
authors.forename: K
authors.initials: K
authors.lastname: Ingram
authors.forename: J
authors.initials: J
authors.lastname: Keiser
authors.forename: J A
authors.initials: JA
authors.lastname: Schifferli
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011956:Receptors, Cell Surface / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D012550:Schistosoma mansoni / Q000276:immunology*; D012552:Schistosomiasis / Q000276:immunology* / Q000517:prevention & control*; D014612:Vaccines / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; D014612:Vaccines