title: Structural features and antioxidant activity of xylooligosaccharides enzymatically produced from sugarcane bagasse.
abstract: Xylooligosaccharides (XOS) were prepared from xylan-rich hemicelluloses isolated by potassium hydroxide from sugarcane bagasse by hydrolysis with crude xylanase secreted by Pichia stipitis. Hydrolysis for 12h produced XOS with a maximum yield of 31.8%, equivalent to 5.29 mg mL(-1) in the hydrolyzate. XOS with degrees of polymerization (DP) from 2 to 4 (xylobiose, xylotriose, and xylotetraose) were the major components in the hydrolysates, whereas the oligosaccharides with higher DP of 5-6 (xylopentaose and xylohexose) showed a constant low level. FT-IR and NMR ((1)H, (13)C, HSQC) demonstrated that XOS contained Araf and 4-O-Me-α-D-GlcpA residues. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay showed that the XOS exhibited concentration-dependent antioxidant activity. The results obtained indicate that the XOS produced from sugarcane bagasse can be employed in food-related applications.
authors.affiliation: Institute of Biomass Chemistry and Technology, Beijing Forestry University, Beijing 100083, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Bian
authors.forename: Feng
authors.initials: F
authors.lastname: Peng
authors.forename: Xiao-Peng
authors.initials: XP
authors.lastname: Peng
authors.forename: Pai
authors.initials: P
authors.lastname: Peng
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.forename: Run-Cang
authors.initials: RC
authors.lastname: Sun
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D001713:Biphenyl Compounds; D002482:Cellulose / Q000378:metabolism*; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D005965:Glucuronates / Q000096:biosynthesis* / Q000737:chemistry; D006868:Hydrolysis; D006878:Hydroxides; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry; D010843:Pichia / Q000201:enzymology; D010851:Picrates; D058105:Polymerization; D011134:Polysaccharides / Q000378:metabolism*; D017680:Potassium Compounds; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D005965:Glucuronates; D006878:Hydroxides; D009844:Oligosaccharides; D010851:Picrates; D011134:Polysaccharides; D017680:Potassium Compounds; C570991:xylooligosaccharide; C007916:hemicellulose; D002482:Cellulose; C027433:bagasse; C004931:1,1-diphenyl-2-picrylhydrazyl; D043364:Endo-1,4-beta Xylanases; C029943:potassium hydroxide