title: The polymorphisms of LYRM1 gene and their association with body measurement and ultrasound traits of Qinchuan cattle.
abstract: Body measurement and meat quality traits which play important roles in the assessment of productivity and economy in cattle were influenced by genes and environmental factors. Latest studies showed that LYR motif containing 1 (LYRM1) may be involved in influencing fatness deposition in animals. The objective of this study was to detect bovine LYRM1 gene polymorphism and analyze its association with body measurement and meat quality traits of cattle. Blood samples were taken from a total of 404 Qinchuan cattle aged from 18-24 months. Created restriction site-polymerase chain reaction-restriction fragment length polymorphism (CRS-PCR-RFLP) and DNA sequencing were used to find out LYRM1 single polymorphism nucleotide (SNPs). Sequence analysis of LYRM1 gene revealed two SNPs (g.165 C > A, g.193 A > G) in 3' untranslated region (3'UTR) of exon 3. And g.165 C > A showed two genotypes namely AC and CC while g.193 A > G showed three genotypes: AA, AG and GG. Analysis results showed that there were significant associations between polymorphism of these two and body measurement and meat quality traits in Qinchuan cattle population. Based on the results obtained from this study, it is inferred that LYRM1 gene may have potential effects on body measurement and meat quality traits in Qinchuan cattle population and could be used for marker-assisted selection.
authors.affiliation: College of Animal Science and Technology, Northwest A & F University, Yangling, 712100, Shaanxi, People's Republic of China.
authors.forename: Yaokun
authors.initials: Y
authors.lastname: Li
authors.forename: Jianbin
authors.initials: J
authors.lastname: Gao
authors.forename: Ning
authors.initials: N
authors.lastname: Yang
authors.forename: Changzhen
authors.initials: C
authors.lastname: Fu
authors.forename: Gong
authors.initials: G
authors.lastname: Cheng
authors.forename: Hongbao
authors.initials: H
authors.lastname: Wang
authors.forename: Linsen
authors.initials: L
authors.lastname: Zan
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000235:genetics*; D001483:Base Sequence; D049628:Body Size / Q000235:genetics*; D002417:Cattle / Q000235:genetics; D063427:Food Quality; D005787:Gene Frequency; D056726:Genetic Association Studies; D008460:Meat / Q000592:standards*; D018482:Muscle, Skeletal / Q000000981:diagnostic imaging; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D050151:Subcutaneous Fat / Q000000981:diagnostic imaging; D014463:Ultrasonography
chemical_list: D051017:Apoptosis Regulatory Proteins