title: Activated charcoal enhanced the antigen-expression and dendritic cell maturation of the vaccine using Listeria-platform.
abstract: BACKGROUND
  Listeria monocytogenes (LM) has been used as a vaccine vector based upon its ability to induce a strong cell-mediated immune response. LM inactivated with γ-irradiation retains immunogenic properties and is an attractive platform for clinical use since it would have improved safety concerns compared to live vectors. Activated charcoal has been shown to enhance expression of LM proteins such as PrfA.
  
  
  OBJECTIVE
  To investigate the effect of various growth conditions supplemented with activated charcoal on recombinant antigen expression.
  
  
  METHODS
  We prepared γ-irradiated ovalbumin-expressing LM (LM-OVA) after growth under various culture conditions. We cultured LM-OVA at various temperatures including 25°C, 37°C and 37°C with activated charcoal and compared OVA expression by western blot analysis, dendritic cells maturation and OVA-specific T cells.
  
  
  RESULTS
  The OVA expression was highest in γ-irradiated LM-OVA grown with activated charcoal at 37°C. Compared to other growth conditions, γ-irradiated LM-OVA grown with activated charcoal at 37°C induce better DC maturation as well as production of the highest number of antigen-specific IFN γ-secreting T cells.
  
  
  CONCLUSIONS
  The further study should be demonstrated the potential to alter growth conditions to enhance OVA expression resulting for vaccine vectors, thereby improving their safety and efficacy.
authors.affiliation: Department of Pathology, College of Medicine and Public Health, Ubon Ratchathani University, Ubon Ratchathani, Thailand. Mdsurawa@ubu.ac.th
authors.forename: Surasak
authors.initials: S
authors.lastname: Wanram
authors.forename: Donald
authors.initials: D
authors.lastname: Guiney
authors.forename: Pawana
authors.initials: P
authors.lastname: Panomket
authors.forename: Patcharee
authors.initials: P
authors.lastname: Jearanaikoon
mesh_terms: D000818:Animals; D015153:Blotting, Western; D018414:CD8-Positive T-Lymphocytes / Q000502:physiology; D002478:Cells, Cultured; D002606:Charcoal; D046508:Culture Techniques; D003713:Dendritic Cells / Q000276:immunology*; D005720:Gamma Rays; D008089:Listeria monocytogenes / Q000502:physiology* / Q000528:radiation effects; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D014614:Vaccines, Synthetic
chemical_list: D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D002606:Charcoal; D010047:Ovalbumin