title: Genetic transformation of cotton with a harpin-encoding gene hpaXoo confers an enhanced defense response against Verticillium dahliae Kleb.
abstract: The soil-borne fungal pathogen Verticillium dahliae Kleb causes Verticillium wilt in a wide range of crops including cotton (Gossypium hirsutum). To date, most upland cotton varieties are susceptible to V. dahliae and the breeding for cotton varieties with the resistance to Verticillium wilt has not been successful. Hpa1Xoo is a harpin protein from Xanthomonas oryzae pv. oryzae which induces the hypersensitive cell death in plants. When hpa1Xoo was transformed into the susceptible cotton line Z35 through Agrobacterium-mediated transformation, the transgenic cotton line (T-34) with an improved resistance to Verticillium dahliae was obtained. Here, we describe the related research approach, such as Western blot, Southern blot, immuno-gold labeling, evaluation of resistance to Verticillium dahliae, and how to detect the micro-hypersensitive response and oxidative burst elicited by harpin(Xoo) in plant tissue.
authors.affiliation: College of Environment and Plant Protection, Hainan University, Haikou, People's Republic of China. weiguomiao1105@yahoo.com.cn
authors.forename: Weiguo
authors.initials: W
authors.lastname: Miao
authors.forename: Jingsheng
authors.initials: J
authors.lastname: Wang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D060467:Disease Resistance / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D005818:Genetic Engineering / Q000379:methods*; D003368:Gossypium / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D006861:Hydrogen Peroxide / Q000378:metabolism; D007613:Kanamycin Resistance / Q000235:genetics; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012988:Soil Microbiology; D014170:Transformation, Genetic; D020172:Verticillium / Q000502:physiology*; D014974:Xanthomonas / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D018744:DNA, Plant; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide