title: Comparison of multilocus sequence typing (MLST) and repetitive sequence-based PCR (rep-PCR) fingerprinting for differentiation of Campylobacter jejuni isolated from broiler in Chiang Mai, Thailand.
abstract: We compared rapid fingerprinting using repetitive sequence-based PCR (rep-PCR) for subtyping Campylobacter jejuni isolates to the widely used multilocus sequence typing (MLST). Representative C. jejuni isolates (n = 16) from broilers were analyzed using MLST and rep-PCR. Both techniques demonstrated an equal discriminatory power of 0.8917, and 9 subgroups were identified. Clonal identification of all 16 isolates was identical for both techniques. The rep-PCR as described in this study may be used as a rapid and cost-effective alternative for subtyping of C. jejuni isolates, or as an effective screening tool in large epidemiological studies.
authors.affiliation: Department of Food Animal Clinic, Chiang Mai University, Thailand.
authors.forename: Prapas
authors.initials: P
authors.lastname: Patchanee
authors.forename: Chomporn
authors.initials: C
authors.lastname: Chokboonmongkol
authors.forename: Karl-Hans
authors.initials: KH
authors.lastname: Zessin
authors.forename: Thomas
authors.initials: T
authors.lastname: Alter
authors.forename: Sarinya
authors.initials: S
authors.lastname: Pornaem
authors.forename: Nipa
authors.initials: N
authors.lastname: Chokesajjawatee
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000191:economics / Q000379:methods; D002169:Campylobacter Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002645:Chickens; D005260:Female; D058885:Multilocus Sequence Typing / Q000191:economics / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D011201:Poultry Diseases / Q000175:diagnosis / Q000382:microbiology*; D012091:Repetitive Sequences, Nucleic Acid; D013785:Thailand