title: Removed antibiotic-impregnated cement spacers in two-stage revision joint arthroplasty do not show biofilm formation in vivo.
abstract: Use of antibiotic-impregnated spacers is common in the two-stage approach to treatment of periprosthetic joint infection despite the lack of information regarding in vivo performance of these implants. Antibiotic elution levels likely often fall below the minimal inhibitory concentration need to inhibit bacterial growth, raising concern that the spacers themselves may provide a potential attachment site for biofilm formation. Advanced microscopy was used in this study to evaluate the surface characteristics of antibiotic-eluting spacers collected at the time of prosthesis reimplantation from 6 patients undergoing two-stage treatment for an infected total joint arthroplasty. Scanning electron microscopy and confocal scanning microscopy of the removed spacers revealed modest fibrous matrix formation and inflammatory cells with no biofilm or bacteria detected. This study supports the continued use of antibiotic spacers in the treatment of periprosthetic joint infection.
authors.affiliation: Department of Orthopaedic Surgery, University of Virginia Health System, Charlottesville, VA 22908, USA.
authors.forename: Justin W
authors.initials: JW
authors.lastname: Griffin
authors.forename: Stacey J
authors.initials: SJ
authors.lastname: Guillot
authors.forename: Jan A
authors.initials: JA
authors.lastname: Redick
authors.forename: James A
authors.initials: JA
authors.lastname: Browne
mesh_terms: D000368:Aged; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D019643:Arthroplasty, Replacement; D018441:Biofilms / Q000187:drug effects*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D019904:Polymethyl Methacrylate; D011446:Prospective Studies; D012086:Reoperation; D013530:Surgical Wound Infection / Q000188:drug therapy
chemical_list: D000900:Anti-Bacterial Agents; D019904:Polymethyl Methacrylate