title: An in vitro study of the photodynamic effect of rose bengal on Trichophyton rubrum.
abstract: Onychomycosis, a fungal infection of the finger or toenails, is predominantly caused by Trichophyton rubrum. Treatment is difficult due to high recurrence rates and problems with treatment compliance. For these reasons, alternative therapies are needed. Here we describe the photoactivation of Rose Bengal (RB) using a green laser (λ = 532 nm) at fluences of 68, 133 and 228 J/cm(2) , and assess its fungicidal activity on T. rubrum spore suspensions. A 140 µM RB solution was able to induce a fungicidal effect on T. rubrum when photosensitized with the fluence of 228 J/cm(2) . RB photosensitization using a green laser provides a potential novel treatment for T. rubrum infections.
authors.affiliation: School of Science and Health, University of Western Sydney, Campbelltown NSW 2560, Australia.
authors.forename: Leah
authors.initials: L
authors.lastname: Cronin
authors.forename: Michelle
authors.initials: M
authors.lastname: Moffitt
authors.forename: Damia
authors.initials: D
authors.lastname: Mawad
authors.forename: Oliver C
authors.initials: OC
authors.lastname: Morton
authors.forename: Antonio
authors.initials: A
authors.lastname: Lauto
authors.forename: Colin
authors.initials: C
authors.lastname: Stack
mesh_terms: D003624:Darkness; D004305:Dose-Response Relationship, Drug; D007834:Lasers; D055620:Optical Phenomena; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D012395:Rose Bengal / Q000494:pharmacology*; D014249:Trichophyton / Q000187:drug effects* / Q000528:radiation effects*
chemical_list: D017319:Photosensitizing Agents; D012395:Rose Bengal
keywords: PDT; fungal infection; laser; onychomycosis