title: High-sensitivity real-time analysis of nanoparticle toxicity in green fluorescent protein-expressing zebrafish.
abstract: Gold nanoparticles (AuNP) show great potential for diagnostic and therapeutic application in humans. A great number of studies have tested the cytotoxicity of AuNP using cell culture. There is, however, an urgent need to test AuNP in vertebrate animal models that interrogate biodistribution and complex biological traits like organ development, whole body metabolism, and cognitive function. The sheer number of different compounds precludes the use of small rodent model for initial screening. The extended fish embryo test (FET) is used here to bridge the gap between cell culture and small animal models. A study on the toxicity of ultrasmall AuNP in wild type and transgenic zebrafish is presented. FET faithfully reproduce all important findings of a previous study in HeLa cells and add new important information on teratogenicity and hepatotoxicity that could not be gained from studying cultured cells.
authors.affiliation: Helmholtz Institute for Biomedical Engineering, Biointerface Laboratory, RWTH Aachen University, Aachen, Germany.
authors.forename: Yu
authors.initials: Y
authors.lastname: Pan
authors.forename: Annika
authors.initials: A
authors.lastname: Leifert
authors.forename: Michael
authors.initials: M
authors.lastname: Graf
authors.forename: Frank
authors.initials: F
authors.lastname: Schiefer
authors.forename: Sven
authors.initials: S
authors.lastname: Thoröe-Boveleth
authors.forename: Janine
authors.initials: J
authors.lastname: Broda
authors.forename: Mary C
authors.initials: MC
authors.lastname: Halloran
authors.forename: Henner
authors.initials: H
authors.lastname: Hollert
authors.forename: Dominic
authors.initials: D
authors.lastname: Laaf
authors.forename: Ulrich
authors.initials: U
authors.lastname: Simon
authors.forename: Willi
authors.initials: W
authors.lastname: Jahnen-Dechent
mesh_terms: D000818:Animals; D006046:Gold / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000235:genetics*; D053768:Metal Nanoparticles / Q000633:toxicity*; D014018:Tissue Distribution; D015027:Zebrafish
chemical_list: D049452:Green Fluorescent Proteins; D006046:Gold