title: Ex vivo evidence for the contribution of hemodynamic shear stress abnormalities to the early pathogenesis of calcific bicuspid aortic valve disease.
abstract: The bicuspid aortic valve (BAV) is the most common congenital cardiac anomaly and is frequently associated with calcific aortic valve disease (CAVD). The most prevalent type-I morphology, which results from left-/right-coronary cusp fusion, generates different hemodynamics than a tricuspid aortic valve (TAV). While valvular calcification has been linked to genetic and atherogenic predispositions, hemodynamic abnormalities are increasingly pointed as potential pathogenic contributors. In particular, the wall shear stress (WSS) produced by blood flow on the leaflets regulates homeostasis in the TAV. In contrast, WSS alterations cause valve dysfunction and disease. While such observations support the existence of synergies between valvular hemodynamics and biology, the role played by BAV WSS in valvular calcification remains unknown. The objective of this study was to isolate the acute effects of native BAV WSS abnormalities on CAVD pathogenesis. Porcine aortic valve leaflets were subjected ex vivo to the native WSS experienced by TAV and type-I BAV leaflets for 48 hours. Immunostaining, immunoblotting and zymography were performed to characterize endothelial activation, pro-inflammatory paracrine signaling, extracellular matrix remodeling and markers involved in valvular interstitial cell activation and osteogenesis. While TAV and non-coronary BAV leaflet WSS essentially maintained valvular homeostasis, fused BAV leaflet WSS promoted fibrosa endothelial activation, paracrine signaling (2.4-fold and 3.7-fold increase in BMP-4 and TGF-β1, respectively, relative to fresh controls), catabolic enzyme secretion (6.3-fold, 16.8-fold, 11.7-fold, 16.7-fold and 5.5-fold increase in MMP-2, MMP-9, cathepsin L, cathepsin S and TIMP-2, respectively) and activity (1.7-fold and 2.4-fold increase in MMP-2 and MMP-9 activity, respectively), and bone matrix synthesis (5-fold increase in osteocalcin). In contrast, BAV WSS did not significantly affect α-SMA and Runx2 expressions and TIMP/MMP ratio. This study demonstrates the key role played by BAV hemodynamic abnormalities in CAVD pathogenesis and suggests the dependence of BAV vulnerability to calcification on the local degree of WSS abnormality.
authors.affiliation: Department of Aerospace and Mechanical Engineering, University of Notre Dame, Notre Dame, Indiana, United States of America.
authors.forename: Ling
authors.initials: L
authors.lastname: Sun
authors.forename: Santanu
authors.initials: S
authors.lastname: Chandra
authors.forename: Philippe
authors.initials: P
authors.lastname: Sucosky
mesh_terms: D000465:Algorithms; D000818:Animals; D001011:Aorta / Q000002:abnormalities / Q000378:metabolism / Q000503:physiopathology*; D001021:Aortic Valve / Q000002:abnormalities / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D017209:Apoptosis; D000082882:Bicuspid Aortic Valve Disease; D001783:Blood Flow Velocity; D015153:Blotting, Western; D055415:Bone Morphogenetic Protein 4 / Q000378:metabolism; D002114:Calcinosis / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D006349:Heart Valve Diseases / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D006439:Hemodynamics; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008955:Models, Cardiovascular; D013314:Stress, Mechanical; D013552:Swine; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014261:Tricuspid Valve / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology
chemical_list: D055415:Bone Morphogenetic Protein 4; D053773:Transforming Growth Factor beta1; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9