title: microRNA changes in the dorsal horn of the spinal cord of rats with             chronic constriction injury: A TaqMan® Low Density Array study.
abstract: Elucidation of the mechanisms underlying neuropathic pain is expected to             aid in the discovery and selection of effective therapeutic methods. Currently,             microRNA (miRNA) is thought to play an important role in the development and maintenance             of the nervous system. We, therefore, hypothesized that miRNAs are involved in             neuropathic pain, and investigated this possibility by analyzing miRNA expression             in the dorsal horn of the spinal cord in a chronic constriction injury (CCI) rat             model using the TaqMan® Low Density Array (TLDA). Neuropathic pain model rats             were produced by CCI induced by ligation of the sciatic nerve. The miRNA expression             in the dorsal horn of the spinal cord was analyzed in Day 0 rats, with no sciatic             nerve ligation or sham operation, Day 7 rats, examined 7 days after sciatic nerve             ligation or sham operation, and Day 14 rats, examined 14 days after sciatic nerve             ligation or sham operation using TLDA. In this study, 111 miRNAs were significantly             regulated in CCI rats in both the Day 7 and Day 14 groups compared with sham rats             in both groups. Of these 111, there were 75 miRNAs (67.6%) that had been analyzed             in previous reports and 36 miRNAs (32.4%) related to the development of tumors             of the nervous system and neurodegenerative diseases. Certain miRNAs were reported             to be related to neuropathic pain; miR-500, -221 and -21. The expression levels             of a large number of miRNAs in the dorsal horn of the spinal cord in CCI rats             changed. These results provide a step toward elucidation of the mechanisms underlying             neuropathic pain.
authors.affiliation: Department of Anaesthesiology and Pain Medicine, Graduate School of Medicine, Nippon Medical School, Bunkyo-ku, Tokyo 113-8603, Japan. y-genda@nms.ac.jp
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Genda
authors.forename: Masae
authors.initials: M
authors.lastname: Arai
authors.forename: Masashi
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Tanaka
authors.forename: Tadashi
authors.initials: T
authors.lastname: Okabe
authors.forename: Atsuhiro
authors.initials: A
authors.lastname: Sakamoto
mesh_terms: D000818:Animals; D059350:Chronic Pain / Q000473:pathology; D003250:Constriction; D003251:Constriction, Pathologic / Q000473:pathology*; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000302:isolation & purification; D009437:Neuralgia / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology*
chemical_list: D035683:MicroRNAs