title: HLA-G haplotype structure shows good conservation between different populations and good correlation with high, normal and low soluble HLA-G expression.
abstract: HLA-G molecule has considerable impact in various clinical fields, therefore many studies attempted to predict its expression based on HLA-G genotype. These studies have focused on polymorphisms in either the coding region or in one of the two untranslated regions (UTR) of the gene. The aim of our study was to determine if HLA-G haplotype defined based on SNPs 5' and 3'UTR could be used to predict soluble HLA-G expression in unstimulated individuals. Our findings showed that HLA-G haplotype structure was well conserved between distant populations and that the defined haplotypes were correlated with high, normal and low HLA-G soluble secretors. In conclusion, we showed that this genotyping strategy based on the use of a few selected SNPs rather than isolated SNP analysis allows reliable HLA-G expression in all populations. This strategy could be useful in a number of clinical settings, e.g., predicting graft compatibility immunogenetic laboratories.
authors.affiliation: Aix-Marseille Université, CNRS, EFS, ADES UMR 7268, 13916 Marseille, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Di Cristofaro
authors.forename: Dima
authors.initials: D
authors.lastname: El Moujally
authors.forename: Anaïs
authors.initials: A
authors.lastname: Agnel
authors.forename: Stéphane
authors.initials: S
authors.lastname: Mazières
authors.forename: Martí
authors.initials: M
authors.lastname: Cortey
authors.forename: Agnès
authors.initials: A
authors.lastname: Basire
authors.forename: Jacques
authors.initials: J
authors.lastname: Chiaroni
authors.forename: Christophe
authors.initials: C
authors.lastname: Picard
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000483:Alleles; D005786:Gene Expression Regulation; D005787:Gene Frequency; D059951:HLA-G Antigens / Q000235:genetics* / Q000378:metabolism*; D006239:Haplotypes; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D020641:Polymorphism, Single Nucleotide
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D059951:HLA-G Antigens