title: Relationship between the expression of the extracellular matrix genes SPARC, SPP1, FN1, ITGA5 and ITGAV and clinicopathological parameters of tumor progression and colorectal cancer dissemination.
abstract: OBJECTIVE
  To evaluate the relationship between the expression of the extracellular matrix (ECM) genes SPARC, SPP1, FN1, ITGA5 and ITGAV and the histopathologic parameters of neoplastic progression and colorectal carcinoma (CRC) dissemination.
  
  
  METHODS
  A retrospective study was conducted in 114 patients with stage I-IV CRC who underwent primary tumor resection. Quantitative real-time PCR and immunohistochemistry (IHC) assays were performed in samples obtained from the primary tumors. The correlation between the expression of these markers and the expression of p53, Bcl-2, Ki67, epidermal growth factor receptor (EGFR) and vascular endothelial growth factor was assessed with the Spearman coefficient (r).
  
  
  RESULTS
  The ITGAV gene was found to be significantly amplified in tumors with positive perineural invasion (p = 0.028). Expression of the SPARC, SPP1, FN1, ITGA5 and ITGAV genes did not correlate with TNM staging. A direct relationship between ITGAV and EGFR expression (r = 0.774; p < 0.001) was observed by IHC.
  
  
  CONCLUSIONS
  ECM gene expression did not correlate with classical prognostic factors for CRC, but overexpression of the ITGAV gene and protein was correlated with an increased risk of perineural invasion. The relationship between ITGAV and EGFR expression suggests the possibility of crosstalk in this signal pathway.
authors.affiliation: Postgraduate Program in Interdisciplinary Surgery, Federal University of São Paulo, São Paulo, Brazil. lsviana1@yahoo.com.br
authors.forename: Luciano de Souza
authors.initials: Lde S
authors.lastname: Viana
authors.forename: Renato José
authors.initials: RJ
authors.lastname: Affonso
authors.forename: Sandra Regina Morini
authors.initials: SR
authors.lastname: Silva
authors.forename: Marcos Vinicius Araujo
authors.initials: MV
authors.lastname: Denadai
authors.forename: Delcio
authors.initials: D
authors.lastname: Matos
authors.forename: Carolina
authors.initials: C
authors.lastname: Salinas de Souza
authors.forename: Jaques
authors.initials: J
authors.lastname: Waisberg
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism*; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D039482:Integrin alpha5 / Q000235:genetics / Q000378:metabolism*; D039564:Integrin alphaV / Q000235:genetics / Q000378:metabolism*; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000556:secondary; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000556:secondary; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D015676:Osteonectin; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism / Q000556:secondary; D010534:Peritoneal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000556:secondary; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046888:Tissue Array Analysis; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C067625:FN1 protein, human; D005353:Fibronectins; D039482:Integrin alpha5; D039564:Integrin alphaV; D015676:Osteonectin; D012333:RNA, Messenger; C577241:SPARC protein, human; C506895:SPP1 protein, human; D025521:Tumor Suppressor Proteins; D053495:Osteopontin