title: IgG expression in human colorectal cancer and its relationship to cancer cell behaviors.
abstract: Increasing evidence indicates that various cancer cell types are capable of producing IgG. The exact function of cancer-derived IgG has, however, not been elucidated. Here we demonstrated the expression of IgG genes with V(D)J recombination in 80 cases of colorectal cancers, 4 colon cancer cell lines and a tumor bearing immune deficient mouse model. IgG expression was associated with tumor differentiation, pTNM stage, lymph node involvement and inflammatory infiltration and positively correlated with the expressions of Cyclin D1, NF-κB and PCNA. Furthermore, we investigated the effect of cancer-derived IgG on the malignant behaviors of colorectal cancer cells and showed that blockage of IgG resulted in increased apoptosis and negatively affected the potential for anchor-independent colony formation and cancer cell invasion. These findings suggest that IgG synthesized by colorectal cancer cells is involved in the development and growth of colorectal cancer and blockage of IgG may be a potential therapy in treating this cancer.
authors.affiliation: Department of Pathology, Weifang Medical University, Weifang, China.
authors.forename: Na
authors.initials: N
authors.lastname: Niu
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Huang
authors.forename: Yingui
authors.initials: Y
authors.lastname: Sun
authors.forename: Zhengshan
authors.initials: Z
authors.lastname: Chen
authors.forename: Weining
authors.initials: W
authors.lastname: Yi
authors.forename: Christine
authors.initials: C
authors.lastname: Korteweg
authors.forename: Juping
authors.initials: J
authors.lastname: Wang
authors.forename: Jiang
authors.initials: J
authors.lastname: Gu
mesh_terms: D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000276:immunology*; D019938:Cyclin D1 / Q000096:biosynthesis; D004195:Disease Models, Animal; D005434:Flow Cytometry / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization; D019169:Jurkat Cells; D051379:Mice; D016513:Mice, SCID; D016328:NF-kappa B / Q000096:biosynthesis; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis
chemical_list: D007074:Immunoglobulin G; D016328:NF-kappa B; D018809:Proliferating Cell Nuclear Antigen; D019938:Cyclin D1