title: Self-assembling complexes of quantum dots and scFv antibodies for cancer cell targeting and imaging.
abstract: Semiconductor quantum dots represent a novel class of fluorophores with unique physical and chemical properties which could enable a remarkable broadening of the current applications of fluorescent imaging and optical diagnostics. Complexes of quantum dots and antibodies are promising visualising agents for fluorescent detection of selective biomarkers overexpressed in tumor tissues. Here we describe the construction of self-assembling fluorescent complexes of quantum dots and anti-HER1 or anti-HER2/neu scFv antibodies and their interactions with cultured tumor cells. A binding strategy based on a very specific non-covalent interaction between two proteins, barnase and barstar, was used to connect quantum dots and the targeting antibodies. Such a strategy allows combining the targeting and visualization functions simply by varying the corresponding modules of the fluorescent complex.
authors.affiliation: Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia. t.zdobnova@mail.ru
authors.forename: Tatiana A
authors.initials: TA
authors.lastname: Zdobnova
authors.forename: Oleg A
authors.initials: OA
authors.lastname: Stremovskiy
authors.forename: Ekaterina N
authors.initials: EN
authors.lastname: Lebedenko
authors.forename: Sergey M
authors.initials: SM
authors.lastname: Deyev
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000378:metabolism; D016466:CHO Cells; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D004586:Electrophoresis; D004926:Escherichia coli / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D009369:Neoplasms / Q000276:immunology* / Q000378:metabolism; D045663:Quantum Dots; D011994:Recombinant Proteins / Q000378:metabolism; D012260:Ribonucleases / Q000737:chemistry; D057127:Single-Chain Antibodies / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: D000906:Antibodies; D001426:Bacterial Proteins; D014408:Biomarkers, Tumor; D005456:Fluorescent Dyes; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies; C057438:barstar protein, Bacillus amyloliquefaciens; D012260:Ribonucleases; C019760:Bacillus amyloliquefaciens ribonuclease