title: CCN2/CTGF binds to fibroblast growth factor receptor 2 and modulates its signaling.
abstract: CCN2 plays a critical role in the development of mesenchymal tissues such as cartilage and bone, and the binding of CCN2 to various cytokines and receptors regulates their signaling.By screening a protein array, we found that CCN2 could bind to fibroblast growth factor receptors (FGFRs) 2 and 3, with a higher affinity toward FGFR2.We ascertained that FGFR2 bound to CCN2 and that the binding of FGFR2 to FGF2 and FGF4 was enhanced by CCN2.CCN2 and FGF2 had a collaborative effect on the phosphorylation of ERK and the differentiation of osteoblastic cells.The present results indicate the biological significance of the binding of CCN2 to FGFR2 in bone metabolism.
authors.affiliation: Biodental Research Center, Dental School, Okayama University, Okayama, Japan.
authors.forename: Eriko
authors.initials: E
authors.lastname: Aoyama
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kubota
authors.forename: Masaharu
authors.initials: M
authors.lastname: Takigawa
mesh_terms: D000818:Animals; D002460:Cell Line; D019902:Chondrocytes / Q000378:metabolism; D055513:Connective Tissue Growth Factor / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D051519:Fibroblast Growth Factor 4 / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D051379:Mice; D010012:Osteogenesis; D010766:Phosphorylation; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000737:chemistry / Q000378:metabolism*; D051498:Receptor, Fibroblast Growth Factor, Type 3 / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D015398:Signal Transduction
chemical_list: C526317:CCN2 protein, human; C496445:FGF4 protein, human; D051519:Fibroblast Growth Factor 4; D011994:Recombinant Proteins; D016222:Fibroblast Growth Factor 2; D055513:Connective Tissue Growth Factor; C496353:FGFR2 protein, human; C496365:FGFR3 protein, human; D051497:Receptor, Fibroblast Growth Factor, Type 2; D051498:Receptor, Fibroblast Growth Factor, Type 3; D048049:Extracellular Signal-Regulated MAP Kinases