title: Modification of polypyrrole nanowires array with platinum nanoparticles and glucose oxidase for fabrication of a novel glucose biosensor.
abstract: A novel glucose biosensor, based on the modification of well-aligned polypyrrole nanowires array (PPyNWA) with Pt nanoparticles (PtNPs) and subsequent surface adsorption of glucose oxidase (GOx), is described. The distinct differences in the electrochemical properties of PPyNWA-GOx, PPyNWA-PtNPs, and PPyNWA-PtNPs-GOx electrodes were revealed by cyclic voltammetry. In particular, the results obtained for PPyNWA-PtNPs-GOx biosensor showed evidence of direct electron transfer due mainly to modification with PtNPs. Optimum fabrication of the PPyNWA-PtNPs-GOx biosensor for both potentiometric and amperometric detection of glucose were achieved with 0.2 M pyrrole, applied current density of 0.1 mA cm(-2), polymerization time of 600 s, cyclic deposition of PtNPs from -200 mV to 200 mV, scan rate of 50 mV s(-1), and 20 cycles. A sensitivity of 40.5 mV/decade and a linear range of 10 μM to 1000 μM (R(2)=0.9936) were achieved for potentiometric detection, while for amperometric detection a sensitivity of 34.7 μA cm(-2) mM(-1) at an applied potential of 700 mV and a linear range of 0.1-9 mM (R(2)=0.9977) were achieved. In terms of achievable detection limit, potentiometric detection achieved 5.6 μM of glucose, while amperometric detection achieved 27.7 μM.
authors.affiliation: NanoScience and Sensor Technology Research Group, School of Applied Sciences and Engineering, Monash University, Churchill, Victoria 3842, Australia.
authors.forename: Guangqing
authors.initials: G
authors.lastname: Xu
authors.forename: Samuel B
authors.initials: SB
authors.lastname: Adeloju
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Wu
authors.forename: Xinyi
authors.initials: X
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D005947:Glucose / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry; D053770:Nanowires / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*
chemical_list: D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D010984:Platinum; D005949:Glucose Oxidase; D005947:Glucose