title: Amino acid signatures in the Ovar-DRB1 peptide-binding pockets are associated with Ovine Pulmonary Adenocarcinoma susceptibility/resistance.
abstract: Potential relationships between amino acid motifs of various alleles of the ovine major histocompatibility complex DR (Ovar-DR) molecule and occurrence of clinical OPA caused by JSRV were investigated in a case-control study. Latxa sheep (n=132) screened for presence/absence of pulmonary OPA lesions were typed for their Ovar-DRB1 2nd exon alleles by PCR and sequence-based typing (PCR-SBT). The polymorphic amino acid residues derived from the obtained 34 DRB1 protein variants were subjected to a logistic regression-based association study. The amino acids at several positions showed significant associations with the presence/absence of pulmonary OPA lesions; some of the residues were located within the peptide binding cleft of the DRB molecule, including pockets P1, P4, P7 and P9.
authors.affiliation: Department of Genetics, Physical Anthropology and Animal Physiology, Faculty of Science and Technology, University of the Basque Country UPV/EHU, Spain. amaia.larruskain@ehu.es
authors.forename: Amaia
authors.initials: A
authors.lastname: Larruskain
authors.forename: Esmeralda
authors.initials: E
authors.lastname: Minguijón
authors.forename: Koldo
authors.initials: K
authors.lastname: Garcia-Etxebarria
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Arostegui
authors.forename: Bernardino
authors.initials: B
authors.lastname: Moreno
authors.forename: Ramón A
authors.initials: RA
authors.lastname: Juste
authors.forename: Begoña M
authors.initials: BM
authors.lastname: Jugo
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D000077192:Adenocarcinoma of Lung; D020816:Amino Acid Motifs / Q000235:genetics; D000818:Animals; D020022:Genetic Predisposition to Disease; D059811:HLA-DRB1 Chains / Q000235:genetics*; D008175:Lung Neoplasms / Q000235:genetics*; D011648:Pulmonary Adenomatosis, Ovine / Q000235:genetics*; D012756:Sheep
chemical_list: D059811:HLA-DRB1 Chains