title: Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Zucchini from Drosophila melanogaster.
abstract: PIWI-interacting RNAs (piRNAs) bind PIWI proteins and silence transposons to maintain the genomic integrity of germ cells. Zucchini (Zuc), a phospholipase D superfamily member, is conserved among animals and is implicated in piRNA biogenesis. However, the underlying mechanism by which Zuc participates in piRNA biogenesis remains elusive. Drosophila melanogaster Zuc (DmZuc) was expressed in Escherichia coli, purified and crystallized. X-ray diffraction data were collected to 1.75 Å resolution. The crystal belonged to space group P2(1), with unit-cell parameters a=55.0, b=71.2, c=56.3 Å, β=107.9°.
authors.affiliation: Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, Tokyo 113-0032, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Fukuhara
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nishimasu
authors.forename: Luc
authors.initials: L
authors.lastname: Bonnefond
authors.forename: Naoki
authors.initials: N
authors.lastname: Matsumoto
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Ishitani
authors.forename: Osamu
authors.initials: O
authors.lastname: Nureki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002846:Chromatography, Affinity; D017124:Conserved Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D004331:Drosophila melanogaster / Q000201:enzymology*; D004722:Endoribonucleases / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D004926:Escherichia coli; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals; D017433:Protein Structure, Secondary; D016415:Sequence Alignment
chemical_list: D029721:Drosophila Proteins; D021382:Protein Sorting Signals; D004722:Endoribonucleases; C521347:Zuc protein, Drosophila