title: Rubber elongation factor (REF), a major allergen component in Hevea brasiliensis latex has amyloid properties.
abstract: REF (Hevb1) and SRPP (Hevb3) are two major components of Hevea brasiliensis latex, well known for their allergenic properties. They are obviously taking part in the biosynthesis of natural rubber, but their exact function is still unclear. They could be involved in defense/stress mechanisms after tapping or directly acting on the isoprenoid biosynthetic pathway. The structure of these two proteins is still not described. In this work, it was discovered that REF has amyloid properties, contrary to SRPP. We investigated their structure by CD, TEM, ATR-FTIR and WAXS and neatly showed the presence of β-sheet organized aggregates for REF, whereas SRPP mainly fold as a helical protein. Both proteins are highly hydrophobic but differ in their interaction with lipid monolayers used to mimic the monomembrane surrounding the rubber particles. Ellipsometry experiments showed that REF seems to penetrate deeply into the monolayer and SRPP only binds to the lipid surface. These results could therefore clarify the role of these two paralogous proteins in latex production, either in the coagulation of natural rubber or in stress-related responses. To our knowledge, this is the first report of an amyloid formed from a plant protein. This suggests also the presence of functional amyloid in the plant kingdom.
authors.affiliation: CNRS, LCPO, UMR 5629, Pessac, France. kberthelot@enscbp.fr
authors.forename: Karine
authors.initials: K
authors.lastname: Berthelot
authors.forename: Sophie
authors.initials: S
authors.lastname: Lecomte
authors.forename: Yannick
authors.initials: Y
authors.lastname: Estevez
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Coulary-Salin
authors.forename: Ahmed
authors.initials: A
authors.lastname: Bentaleb
authors.forename: Christophe
authors.initials: C
authors.lastname: Cullin
authors.forename: Alain
authors.initials: A
authors.lastname: Deffieux
authors.forename: Frédéric
authors.initials: F
authors.lastname: Peruch
mesh_terms: D000485:Allergens; D000682:Amyloid / Q000737:chemistry*; D003001:Cloning, Molecular; D019286:Endopeptidase K / Q000737:chemistry; D005456:Fluorescent Dyes / Q000494:pharmacology; D028482:Hevea / Q000378:metabolism*; D007840:Latex / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry; D046529:Microscopy, Electron, Transmission / Q000379:methods; D008969:Molecular Sequence Data; D010445:Peptide Elongation Factors / Q000737:chemistry* / Q000276:immunology*; D010802:Phylogeny; D011108:Polymers / Q000737:chemistry; D011485:Protein Binding; D017433:Protein Structure, Secondary; D012408:Rubber / Q000737:chemistry*; D012542:Scattering, Radiation; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013499:Surface Properties; D014965:X-Rays
chemical_list: D000485:Allergens; D000682:Amyloid; D005456:Fluorescent Dyes; D007840:Latex; D008055:Lipids; D010445:Peptide Elongation Factors; D011108:Polymers; D012408:Rubber; D019286:Endopeptidase K