title: Protective effect of BAG5 on MPP+-induced apoptosis in PC12 cells.
abstract: OBJECTIVE
  Parkinson's disease (PD) is the most common neurodegenerative disease in humans, and an abundance of evidence has implicated apoptosis signaling pathways in the neurodegeneration of PD. The purpose of this study was to assess the role of B-cell lymphoma 2 (Bcl-2)-associated  athanogene 5 (BAG5) protein, which was previously confirmed to play an important role in the pathogenesis of PD, in the regulation of apoptosis induced by 1-methyl-4-phenyl-pyridinium (MPP(+)) in PC12 cells.
  
  
  METHODS
  PC12 cells were treated with MPP(+) for 48 hours  to induce apoptosis, and activation of Bcl-2, Bcl-xl, and caspase 3 was measured by western blot.
  
  
  RESULTS
  The upregulation of BAG5 in PC12 cells inhibited apoptosis and increased the expression of anti-apoptotic proteins, including Bcl-2 and Bcl-xl, after MPP(+) treatment.  In addition, downregulation of BAG5 in PC12 cells enhanced apoptosis and decreased the expression of these proteins after MPP(+) treatment.
  
  
  CONCLUSIONS
  The data suggest that BAG5 inhibits MPP(+)-induced apoptosis through both endogenous and mitochondria-mediated  pathways of apoptosis. Through this mechanism, the upregulation of BAG5 levels may occur through its anti-apoptotic activity in PD.
authors.affiliation: The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Ma
authors.forename: Xuejing
authors.initials: X
authors.lastname: Wang
authors.forename: Xuebing
authors.initials: X
authors.lastname: Ding
authors.forename: Jing
authors.initials: J
authors.lastname: Jing
authors.forename: Yaohua
authors.initials: Y
authors.lastname: Ma
authors.forename: Junfang
authors.initials: J
authors.lastname: Teng
mesh_terms: D015655:1-Methyl-4-phenylpyridinium / Q000037:antagonists & inhibitors / Q000633:toxicity*; D048868:Adaptor Proteins, Signal Transducing / Q000502:physiology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology*; D019610:Cytoprotection / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016716:PC12 Cells; D051381:Rats
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C496514:Bag5 protein, rat; D015655:1-Methyl-4-phenylpyridinium