title: Reduction of type IV collagen by upregulated miR-29 in normal elderly mouse and klotho-deficient, senescence-model mouse.
abstract: MicroRNA (miRNA), a small non-coding RNA that functions as a mediator in gene silencing, plays important roles in gene regulation in various vital functions and activities. Here we show that the miR-29 members are upregulated in klotho-deficient [klotho(-/-)] mice, a senescence-model animal, and also in normal elderly ICR mice relative to wild-type littermates and young ICR mice. In addition, levels of type IV collagen, a major component of basement membranes and a putative target of miR-29, were lower in klotho(-/-) and elderly ICR mice than in wild-type littermates and young ICR mice. RNA degradation mediated by miR-29 may participate in the suppression of type IV collagen, both in vivo and in vitro. Taken together, our current findings suggest that the miR-29 upregulated in aging may be involved in the downregulation of type IV collagen, leading to a possible weakening of the basal membrane in senescent tissues, and miR-29 may be a useful molecular marker of senescence.
authors.affiliation: National Institute of Neuroscience, NCNP, Kodaira, Tokyo, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Takahashi
authors.forename: Akiko
authors.initials: A
authors.lastname: Eda
authors.forename: Tatsunobu
authors.initials: T
authors.lastname: Fukushima
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Hohjoh
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000375:Aging / Q000235:genetics*; D000818:Animals; D024141:Collagen Type IV / Q000378:metabolism*; D003404:Creatinine / Q000097:blood; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D017930:Genes, Reporter; D005966:Glucuronidase / Q000172:deficiency* / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D000090265:Klotho Proteins; D008239:Lysine / Q000097:blood; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008954:Models, Biological; D015854:Up-Regulation / Q000235:genetics*
chemical_list: D024141:Collagen Type IV; C547014:MIRN29 microRNA, mouse; D035683:MicroRNAs; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003404:Creatinine; D005966:Glucuronidase; D000090265:Klotho Proteins; D003849:Deoxyguanosine; D008239:Lysine