title: Characterization of carbon nanotube protein corona by using quantitative proteomics.
abstract: The protein corona of a nanomaterial is a complex layer of proteins spontaneously and stably formed when the material is exposed to body fluids or intracellular environments. In this study, we utilised stable isotope labeling by amino acids in cell culture (SILAC)-based quantitative proteomics to characterise the binding of human cellular proteins to two forms of carbon nanoparticles: namely multi-walled carbon nanotubes (MWCNTs) and carbon black (CB). The relative binding efficiency of over 750 proteins to these materials is measured. The data indicate that MWCNTs and CB bind to vastly different sets of proteins. The molecular basis of selectivity in protein binding is investigated. This study is the first large-scale characterisation of protein corona on CNT, providing the biochemical basis for the assessment of the suitability of CNTs as biomedical tools, and as an emerging pollutant.
  
  
  BACKGROUND
  This team of investigators performed the first large-scale characterization of protein corona on carbon nanotubes, studying 750 proteins and assessing the suitability of CNTs as biomedical tools and as an emerging pollutant.
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon Tong, Hong Kong.
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Cai
authors.forename: Rajkumar
authors.initials: R
authors.lastname: Ramalingam
authors.forename: Hau San
authors.initials: HS
authors.lastname: Wong
authors.forename: Jinping
authors.initials: J
authors.lastname: Cheng
authors.forename: Paul
authors.initials: P
authors.lastname: Ajuh
authors.forename: Shuk Han
authors.initials: SH
authors.lastname: Cheng
authors.forename: Yun Wah
authors.initials: YW
authors.lastname: Lam
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry*; D002460:Cell Line; D006801:Humans; D007553:Isotope Labeling; D053758:Nanoparticles / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics; D053260:Soot / Q000737:chemistry
chemical_list: D000596:Amino Acids; D037742:Nanotubes, Carbon; D011506:Proteins; D053260:Soot; D002244:Carbon