title: Chromatographic evaluation of a newly designed peptide-silica stationary phase in reverse phase liquid chromatography and hydrophilic interaction liquid chromatography: mixed mode behavior.
abstract: The short peptide Boc-Phe-Aib-Phe-OH was synthesized and immobilized onto porous silica using grafting methodology. The resulting peptide-bonded silica was characterized using DRIFT-mode FT-IR, elemental analysis, thermogravimetric analysis, solid state C(13) NMR spectroscopy and the successful immobilization of the peptide on the silica support was confirmed. This grafted phase was packed into a stainless steel column and used for mixed-mode chromatography such as reversed-phase high-performance liquid chromatography and hydrophilic interaction liquid chromatography for the efficient separation of hydrophobic compounds, small polar molecules, and drug molecules. Compared with ODS and phenyl columns, this new stationary phase shows considerably higher molecular-planarity selectivity towards polyaromatic hydrocarbons and also available for separation of nucleo-analytes and sulfa-drug molecules in a hydrophilic interaction liquid chromatography mode. The multiple interactions induced by polar carbonyl group and hydrophobic phenyl group allow this peptide-modified silica to serve as a multi-mode stationary phase in high performance liquid chromatography.
authors.affiliation: Department of Applied Chemistry and Biochemistry, Kumamoto University, 2-39-1 Kurokami Chuo-ku, Kumamoto 860-8555, Japan.
authors.forename: Sudipta
authors.initials: S
authors.lastname: Ray
authors.forename: Makoto
authors.initials: M
authors.lastname: Takafuji
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Ihara
mesh_terms: D000621:Aminoisobutyric Acids / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation* / Q000379:methods; D057927:Hydrophobic and Hydrophilic Interactions; D008956:Models, Chemical; D019906:Nuclear Magnetic Resonance, Biomolecular; D009842:Oligopeptides / Q000737:chemistry*; D010649:Phenylalanine / Q000737:chemistry; D011084:Polycyclic Aromatic Hydrocarbons / Q000302:isolation & purification; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013237:Stereoisomerism; D013256:Steroids / Q000302:isolation & purification; D013457:Sulfur Compounds / Q000302:isolation & purification; D013818:Thermogravimetry
chemical_list: D000621:Aminoisobutyric Acids; D009842:Oligopeptides; D011084:Polycyclic Aromatic Hydrocarbons; D013256:Steroids; D013457:Sulfur Compounds; D010649:Phenylalanine; D012822:Silicon Dioxide