title: Estimated copy number of Bacillus anthracis plasmids pXO1 and pXO2 using digital PCR.
abstract: We evaluated digital PCR (dPCR) to directly enumerate plasmid and chromosome copies in three strains of Bacillus anthracis. Copy number estimates based on conventional quantitative PCR (qPCR) highlighted the variability of using qPCR to measure copy number whereas estimates based on direct sequencing are comparable to dPCR.
authors.affiliation: Chemical and Biological Signature Sciences Group, National Security Directorate, Pacific Northwest National Laboratory, Richland, WA 99354, United States. Timothy.Straub@pnnl.gov
authors.forename: Timothy
authors.initials: T
authors.lastname: Straub
authors.forename: Cheryl
authors.initials: C
authors.lastname: Baird
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Bartholomew
authors.forename: Heather
authors.initials: H
authors.lastname: Colburn
authors.forename: Derrick
authors.initials: D
authors.lastname: Seiner
authors.forename: Kristin
authors.initials: K
authors.lastname: Victry
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Cynthia J
authors.initials: CJ
authors.lastname: Bruckner-Lea
mesh_terms: D001408:Bacillus anthracis / Q000235:genetics*; D018628:Gene Dosage; D008967:Molecular Biology / Q000379:methods*; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*