title: Enhancement of lipopeptides production in a two-temperature-stage process under SSF conditions and its bioprocess in the fermenter.
abstract: A two-temperature-stage process was developed for the production of lipopeptides under SSF conditions. The effects of various temperatures, ranging from 25 to 40 °C, on the bacterial growth during the growth stage and on the production of lipopeptides during the productive stage were investigated. The optimum temperatures were found to be 30 °C for the growth of the strain and 37 °C for the biosynthesis of lipopeptides. The two-stage fermentation temperatures should be 30 °C in the initial 24h and then 37 °C for the enhanced production of lipopeptides. The bioprocess results obtained in a 50 L fermenter verified the efficacy of this technique, which increased the yield of lipopeptides by 8.40% in flasks and by 13.11% in the fermenter, with a 4h decrease of fermentation time in the fermenter. The 1000-fold scale-up of fermentation in a fermenter was successfully achieved.
authors.affiliation: Jiangsu Key Lab for Organic Solid Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China. zzenabcd@126.com
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhu
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.forename: Guanghui
authors.initials: G
authors.lastname: Yu
authors.forename: Wei
authors.initials: W
authors.lastname: Ran
authors.forename: Qirong
authors.initials: Q
authors.lastname: Shen
mesh_terms: D000383:Agriculture; D001407:Bacillus / Q000254:growth & development / Q000378:metabolism*; D019149:Bioreactors; D005285:Fermentation; D055666:Lipopeptides / Q000096:biosynthesis*; D013696:Temperature; D014866:Waste Products / Q000032:analysis*
chemical_list: D055666:Lipopeptides; D014866:Waste Products