title: [Study of genetic susceptibility in 198 children with asthma].
abstract: OBJECTIVE
  To analyze the frequency distribution of single nucleotide polymorphisms (SNPs) of four asthma-related gene loci (ACE I/D; ADRB2 Arg16Gly; TNF-α G-308A; MS4A2 Glu237Gly) in 198 asthmatic children, and to investigate its association with genetic susceptibility to childhood asthma and some clinical phenotypes of asthma.
  
  
  METHODS
  Polymerase chain reaction product electrophoresis identification and real-time quantitative PCR detecting system were used to determine the frequency distributions of the SNPs of the four asthma-related gene loci in 198 asthmatic children and 110 healthy controls. The serum total IgE (TIgE) levels and blood eosinophil proportion (%EOS) of the asthmatic children were measured. Different genotypes at the four asthma-related gene loci were compared in terms of TIgE and %EOS.
  
  
  RESULTS
  The genotype DD of angiotensin-converting enzyme (ACE) had a significantly higher frequency in the asthmatic children than in the healthy controls (χ2= 30.667, P<0.01), and the frequency of D allele was also significantly higher in the asthmatic children than in the healthy controls (χ2=7.151, P<0.01). No correlation was found between the polymorphism of each gene locus and serum TIgE level and %EOS (P>0.05).
  
  
  CONCLUSIONS
  Genotype DD of ACE is related to genetic susceptibility to childhood asthma and may be the risk factor for childhood asthma.
authors.affiliation: Department of Pediatrics, Bo'ai Hospital of Zhongshan City, Zhongshan, Guangdong 528403, China. 13924973891@139.com
authors.forename: Bo-Qiang
authors.initials: BQ
authors.lastname: Zheng
authors.forename: Gui-Lan
authors.initials: GL
authors.lastname: Wang
authors.forename: Sai
authors.initials: S
authors.lastname: Yang
authors.forename: Yan-Qiang
authors.initials: YQ
authors.lastname: Lu
authors.forename: Rui-Jun
authors.initials: RJ
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
mesh_terms: D001249:Asthma / Q000209:etiology / Q000235:genetics*; D002675:Child, Preschool; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics; D017455:Receptors, IgE / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: C502447:MS4A2 protein, human; D018343:Receptors, Adrenergic, beta-2; D017455:Receptors, IgE; D014409:Tumor Necrosis Factor-alpha; D007703:Peptidyl-Dipeptidase A