title: Time-lapse epifluorescence imaging of expressed cRNA to cyclin B1 for studying meiosis I in mouse oocytes.
abstract: The first meiotic division of mammalian oocytes physiologically occurs in the ovary in the hours preceding ovulation. Fortunately, oocytes removed from their follicular environment will readily undergo this process in culture. Their large size, optical transparency, and efficiency in translating exogenous cRNA make mouse oocytes very amenable to study this process in detail using fluorescence imaging-based techniques. Here we describe the process of microinjecting cRNA to proteins of interest that have been coupled to a fluorescent protein using cyclin B1 as an example.
authors.affiliation: School of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW, Australia.
authors.forename: Janet E
authors.initials: JE
authors.lastname: Holt
authors.forename: Simon I R
authors.initials: SI
authors.lastname: Lane
authors.forename: Keith T
authors.initials: KT
authors.lastname: Jones
mesh_terms: D000818:Animals; D002469:Cell Separation; D056744:Cyclin B1 / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005456:Fluorescent Dyes / Q000378:metabolism; D005786:Gene Expression Regulation; D008540:Meiosis; D051379:Mice; D008845:Microinjections; D055616:Microtechnology; D057054:Molecular Imaging / Q000295:instrumentation / Q000379:methods*; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism*; D011247:Pregnancy; D018075:RNA, Complementary / Q000235:genetics*; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D056744:Cyclin B1; D005456:Fluorescent Dyes; D018075:RNA, Complementary