title: Rigid matrix supports osteogenic differentiation of stem cells from human exfoliated deciduous teeth (SHED).
abstract: Stem cell fate can be induced by the grade of stiffness of the extracellular matrix, depending on the developed tissue or complex tissues. For example, a rigid extracellular matrix induces the osteogenic differentiation in bone marrow derived mesenchymal stem cells (MSCs), while a softer surface induces the osteogenic differentiation in dental follicle cells (DFCs). To determine whether differentiation of ectomesenchymal dental precursor cells is supported by similar grades of extracellular matrices (ECMs) stiffness, we examined the influence of the surface stiffness on the proliferation and osteogenic differentiation of stem cells from human exfoliated deciduous teeth (SHED). Cell proliferation of SHED was significantly decreased on cell culture surfaces with a muscle-like stiffness. A dexamethasone-based differentiation medium induced the osteogenic differentiation of SHED on substrates of varying mechanical stiffness. Here, the hardest surface improved the induction of osteogenic differentiation in comparison to that with the softest stiffness. In conclusion, our study showed that the osteogenic differentiation of ectomesenchymal dental precursor cells SHED and DFCs are not supported by similar grades of ECM stiffness.
authors.affiliation: Department of Oral and Maxillofacial Surgery, University Hospital Regensburg, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Viale-Bouroncle
authors.forename: Martin
authors.initials: M
authors.lastname: Gosau
authors.forename: Kevin
authors.initials: K
authors.lastname: Küpper
authors.forename: Christoph
authors.initials: C
authors.lastname: Möhl
authors.forename: Gero
authors.initials: G
authors.lastname: Brockhoff
authors.forename: Torsten E
authors.initials: TE
authors.lastname: Reichert
authors.forename: Gottfried
authors.initials: G
authors.lastname: Schmalz
authors.forename: Tobias
authors.initials: T
authors.lastname: Ettl
authors.forename: Christian
authors.initials: C
authors.lastname: Morsczeck
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D003795:Dental Sac / Q000166:cytology; D003907:Dexamethasone; D005109:Extracellular Matrix / Q000737:chemistry*; D006244:Hardness; D006801:Humans; D010012:Osteogenesis; D013234:Stem Cells / Q000166:cytology*; D014094:Tooth, Deciduous / Q000166:cytology*
chemical_list: D003907:Dexamethasone