title: Inhibition of prostaglandin D₂ production by trihydroxy fatty acids isolated from Ulmus davidiana var. japonica.
abstract: The stem and root barks of Ulmus davidiana var. japonica (Ulmaceae) have been used to treat inflammatory diseases including mastitis, rhinitis, sinusitis, and enteritis. In an ongoing study focused on the discovery of natural anti-inflammatory compounds from natural products, a methanol extract of the stem and root barks of U. davidiana var. japonica showed anti-inflammatory activities. Activity-guided fractionation of the methanol extract yielded a new trihydroxy fatty acid, 9,12,13-trihydroxyoctadeca-10(Z),15(Z)-dienoic acid (1), and a known compound, pinellic acid (2). These two trihydroxy fatty acids 1 and 2 inhibited prostaglandin D₂ production with IC₅₀ values of 25.8 and 40.8 μM, respectively. These results suggest that 9,12,13-trihydroxyoctadeca-10(Z),15(Z)-dienoic acid (1) and pinellic acid (2) are among the anti-inflammatory principles in this medicinal plant.
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea.
authors.forename: Hyun Gyu
authors.initials: HG
authors.lastname: Choi
authors.forename: Yu Mi
authors.initials: YM
authors.lastname: Park
authors.forename: Yue
authors.initials: Y
authors.lastname: Lu
authors.forename: Hyeun Wook
authors.initials: HW
authors.lastname: Chang
authors.forename: Minkyun
authors.initials: M
authors.lastname: Na
authors.forename: Seung Ho
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D005227:Fatty Acids / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D008297:Male; D051379:Mice; D009829:Oleic Acids / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D015230:Prostaglandin D2 / Q000037:antagonists & inhibitors*; D034403:Ulmus / Q000737:chemistry*
chemical_list: C514414:9,12,13-trihydroxyoctadeca-10(E),15(Z)-dienoic acid; D000893:Anti-Inflammatory Agents; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D009829:Oleic Acids; D010936:Plant Extracts; C478149:pinellic acid; D015230:Prostaglandin D2
keywords: 9,12,13-trihydroxyoctadeca-10(Z),15(Z)-dienoic acid; Ulmus davidiana var. japonica; pinellic acid; prostaglandin D2; trihydroxy fatty acid