title: Kisspeptin-10 induces dose dependent degeneration in prepubertal rat prostate gland.
abstract: BACKGROUND
  Kisspeptin peptides mediate their actions through the GnRH loop system. How kisspeptins affect prostate gland in prepubertal male mammals remains elusive.
  
  
  METHODS
  To address this kisspeptin was administered as subchronic (12 days) twice daily i.p. dose at three different dosage regimens: 10 pg, 1 ng and 1 µg, to prepubertal male Sprague-Dawley rats (PND 35). Control rats were maintained in parallel. At the end of the experiment prostate gland was dissected out and processed for light and electron microscopy. DNA damage was also estimated by DNA ladder assay and DNA fragmentation assay.
  
  
  RESULTS
  Prostate weights decreased significantly (P < 0.05) at 1 µg treatment dose of kisspeptin. The epithelial height of secretory acini of prostate decreased at 10 pg (P < 0.05), 1 ng, and 1 µg doses (P < 0.001). Histomorphology and ultrastructure demonstrated, decrease in epithelial cell height, epithelial folding and dilatation of the organelles with kisspeptin treatment. Percent DNA damage to the prostatic tissue was 20.74 ± 2.18, 43.60 ± 2.39, and 58.18 ± 2.59 at 10 pg, 1 ng and 1 µg doses, respectively.
  
  
  CONCLUSIONS
  The study reveals that continuous administration of kisspeptin does not lead to an early maturation but instead severe degeneration of prepubertal prostate gland. Wiley Periodicals, Inc.
authors.affiliation: Gomal Centre of Biochemistry and Biotechnology, Gomal University, Dera Ismail Khan, Pakistan. faiqah_ramzan@yahoo.com
authors.forename: Faiqah
authors.initials: F
authors.lastname: Ramzan
authors.forename: Irfan Zia
authors.initials: IZ
authors.lastname: Qureshi
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ramzan
authors.forename: Muhammad Haris
authors.initials: MH
authors.lastname: Ramzan
authors.forename: Faiza
authors.initials: F
authors.lastname: Ramzan
mesh_terms: D000818:Animals; D004249:DNA Damage / Q000187:drug effects*; D053938:DNA Fragmentation / Q000187:drug effects*; D059648:Kisspeptins / Q000008:administration & dosage*; D008297:Male; D011467:Prostate / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012741:Sexual Maturation / Q000187:drug effects*
chemical_list: C491406:Kiss1 protein, rat; D059648:Kisspeptins