title: Single-step cloning of a hybridoma producing a monoclonal antibody against a target protein.
abstract: We have developed a simple and time-saving method to identify hybridoma clones producing an antibody against a target protein among a large number of hybridomas in a single step. This method is very useful as the primary screening tool for hybridomas producing monoclonal antibodies if several micrograms of the target protein is available.
authors.affiliation: Department of Life Science, University of Hyogo, Akou-gun, Hyogo 678-1297, Japan. orii@sci.u-hyogo.ac.jp
authors.forename: Hidefumi
authors.initials: H
authors.lastname: Orii
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Kenji
authors.initials: K
authors.lastname: Watanabe
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism*; D000941:Antigens / Q000276:immunology; D015153:Blotting, Western; D002469:Cell Separation; D003101:Collodion / Q000737:chemistry; D006825:Hybridomas / Q000166:cytology* / Q000378:metabolism; D007150:Immunohistochemistry; D008163:Luminescent Measurements
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D003101:Collodion