title: Development of genomic and EST-SSR markers in radish (Raphanus sativus L.).
abstract: Radish (Raphanus sativus L.) belongs to Brassicaceae family and is a close relative of Brassica. This species shows a wide morphological diversity, and is an important vegetable especially in Asia. However, molecular research of radish is behind compared to that of Brassica. For example, reports on SSR (simple sequence repeat) markers are limited. Here, we designed 417 radish SSR markers from SSR-enriched genomic libraries and the cDNA data. Of the 256 SSR markers succeeded in PCR, 130 showed clear polymorphisms between two radish lines; a rat-tail radish and a Japanese cultivar, 'Harufuku'. As a test case for evaluation of the present SSRs, we conducted two studies. First, we selected 16 SSRs to calculate polymorphism information contents (PICs) using 16 radish cultivars and four other Brassicaceae species. These markers detected 3-15 alleles (average = 9.6). PIC values ranged from 0.54 to 0.92 (average = 0.78). Second, part of the present SSRs were tested for mapping using our previously-examined mapping population. The map spanned 672.7 cM with nine linkage groups (LGs). The 21 radish SSR markers were distributed throughout the LGs. The SSR markers developed here would be informative and useful for genetic analysis in radish and its related species.
authors.affiliation: Graduate School of Life and Environmental Sciences, Kyoto Prefectural University , 74 Oji, Kitainayazuma, Seika, Soraku, Kyoto 619-0244, Japan.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Nakatsuji
authors.forename: Tomoko
authors.initials: T
authors.lastname: Hashida
authors.forename: Naoko
authors.initials: N
authors.lastname: Matsumoto
authors.forename: Masato
authors.initials: M
authors.lastname: Tsuro
authors.forename: Nakao
authors.initials: N
authors.lastname: Kubo
authors.forename: Masashi
authors.initials: M
authors.lastname: Hirai
keywords: Radish; Raphanus sativus; SSR (simple sequence repeat) marker