title: Three dimensional, sheathless, and high-throughput microparticle inertial focusing through geometry-induced secondary flows.
abstract: A novel inertial focusing platform creates a single-stream microparticle train in a single-focal plane without sheath fluids and external forces, all in a high-throughput manner. The proposed design consists of a low-aspect-ratio straight channel interspersed with a series of constrictions in height arranged orthogonally, making use of inertial focusing and geometry-induced secondary flows. Focusing efficiency as high as 99.77% is demonstrated with throughput as high as 36 000 particles s(-1) for a variety of different sized particles and cells.
authors.affiliation: Department of Bioengineering, California NanoSystems Institute, University of California, Los Angeles, CA 90095, USA.
authors.forename: Aram J
authors.initials: AJ
authors.lastname: Chung
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Gossett
authors.forename: Dino
authors.initials: D
authors.lastname: Di Carlo