title: Potential of Ophiostoma piceae sterol esterase for biotechnologically relevant hydrolysis reactions.
abstract: The ascomycete Ophiostoma piceae produces a sterol esterase (OPE) with high affinity toward p-nitrophenol, glycerol, and sterol esters. Recently, this enzyme has been heterologously expressed in the methylotrophic yeast Pichia pastoris under the AOX1 methanol-inducible promoter (PAOX1) using sorbitol as co-susbtrate, and the hydrolytic activity of the recombinant protein (OPE*) turned out to be improved from a kinetic point of view. In this study, we analyze the effects of sorbitol during the expression of OPE*, at first added as an additional carbon source, and methanol as inducer. The O. piceae enzyme was successfully used for PVAc hydrolysis, suggesting its potential applicability in recycled paper production to decrease stickies problems.
authors.affiliation: Centro de Investigaciones Biológicas CIB, Spanish National Research Council CSIC Madrid, Spain.
authors.forename: Víctor
authors.initials: V
authors.lastname: Barba Cedillo
authors.forename: Alicia
authors.initials: A
authors.lastname: Prieto
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Martínez
mesh_terms: D001709:Biotechnology / Q000379:methods*; D006868:Hydrolysis; D055156:Ophiostoma / Q000201:enzymology*; D002787:Sterol Esterase / Q000378:metabolism*
chemical_list: D002787:Sterol Esterase
keywords: Pichia pastoris; methanol; polyvinyl acetate; recombinant protein; recycled paper; sorbitol; stickies