title: Salvianolic Acid B reducing portal hypertension depends on macrophages in isolated portal perfused rat livers with chronic hepatitis.
abstract: This study is aimed to investigate the effects of Sal B on portal hypertension (PH). PH with chronic hepatitis was induced by carbon tetrachloride (CCl(4)) in rats. The model was confirmed with elevated portal pressures and increased serum CD163 levels. The inducible nitric oxide synthase (iNOS) or heme oxygenase-1 (HO-1) in portal triads was assessed. The isolated portal perfused rat liver (IPPRL) was performed at d(0), d(28), d(56) , and d(84) in the progression of chronic hepatitis. After constricting with phenylephrine, the portal veins were relaxed with Sal B. The EC(50) of Sal B for relaxing portal veins was -2.04 × 10(-9), 7.28 × 10(-11), 1.52 × 10(-11), and 8.44 × 10(-11) mol/L at d(0), d(28), d(56), and d(84), respectively. More macrophages infiltrated in portal triads and expressed more iNOS or HO-1 as PH advanced. The areas under the curve (AUCs) of Sal B for reducing PH were positively correlated with the levels of iNOS or HO-1 in portal triads, and so did with serum CD163 levels. Sal B reduces PH in IPPRL with chronic hepatitis, via promoting portal relaxation due to macrophage-originated NO or CO in portal triads, partly at least.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Hongmei
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authors.lastname: Jia
authors.forename: Shijun
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authors.lastname: Yang
authors.forename: Yuetao
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authors.lastname: Liu
authors.forename: Bo
authors.initials: B
authors.lastname: Deng
authors.forename: Xueyan
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authors.lastname: Xu
authors.forename: Tao
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authors.lastname: Zhang
authors.forename: Hang
authors.initials: H
authors.lastname: Zhou
authors.forename: Chengzhe
authors.initials: C
authors.lastname: Zu
authors.forename: He
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authors.lastname: Yin
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.forename: Yijun
authors.initials: Y
authors.lastname: Song
authors.forename: Yueqi
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authors.lastname: Wang
authors.forename: Pengtao
authors.initials: P
authors.lastname: Li
authors.forename: Zhongmei
authors.initials: Z
authors.lastname: Zou
authors.forename: Dayong
authors.initials: D
authors.lastname: Cai