title: Dual functions of α-ketoglutarate dehydrogenase E2 in the Krebs cycle and mitochondrial DNA inheritance in Trypanosoma brucei.
abstract: The dihydrolipoyl succinyltransferase (E2) of the multisubunit α-ketoglutarate dehydrogenase complex (α-KD) is an essential Krebs cycle enzyme commonly found in the matrices of mitochondria. African trypanosomes developmentally regulate mitochondrial carbohydrate metabolism and lack a functional Krebs cycle in the bloodstream of mammals. We found that despite the absence of a functional α-KD, bloodstream form (BF) trypanosomes express α-KDE2, which localized to the mitochondrial matrix and inner membrane. Furthermore, α-KDE2 fractionated with the mitochondrial genome, the kinetoplast DNA (kDNA), in a complex with the flagellum. A role for α-KDE2 in kDNA maintenance was revealed in α-KDE2 RNA interference (RNAi) knockdowns. Following RNAi induction, bloodstream trypanosomes showed pronounced growth reduction and often failed to equally distribute kDNA to daughter cells, resulting in accumulation of cells devoid of kDNA (dyskinetoplastic) or containing two kinetoplasts. Dyskinetoplastic trypanosomes lacked mitochondrial membrane potential and contained mitochondria of substantially reduced volume. These results indicate that α-KDE2 is bifunctional, both as a metabolic enzyme and as a mitochondrial inheritance factor necessary for the distribution of kDNA networks to daughter cells at cytokinesis.
authors.affiliation: Department of Cellular Biology, University of Georgia, Athens, Georgia, USA.
authors.forename: Steven E
authors.initials: SE
authors.lastname: Sykes
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Hajduk
mesh_terms: D002478:Cells, Cultured; D002952:Citric Acid Cycle; D048749:Cytokinesis; D004261:DNA Replication; D018105:DNA, Kinetoplast / Q000235:genetics*; D004795:Enzyme Stability; D005407:Flagella / Q000378:metabolism; D015870:Gene Expression; D015971:Gene Expression Regulation, Enzymologic; D055785:Gene Knockdown Techniques; D007655:Ketoglutarate Dehydrogenase Complex / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000201:enzymology / Q000235:genetics; D011485:Protein Binding; D021381:Protein Transport; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D034622:RNA Interference; D014346:Trypanosoma brucei brucei / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development
chemical_list: D018105:DNA, Kinetoplast; D015800:Protozoan Proteins; D007655:Ketoglutarate Dehydrogenase Complex