title: Evaluation of core-shell particle columns for ion-pair reversed-phase liquid chromatography analysis of oligonucleotides.
abstract: An investigation into the use of core-shell particle columns for separation of short (∼21 base pairs) RNA oligonucleotides by ion-pair reversed-phase liquid chromatography (IP-RPLC) showed improved resolution for a number of test analytes relative to conventional (fully-porous) reversed-phase columns. The best resolutions were obtained using columns packed with smaller sub-2μm core-shell particles.
authors.affiliation: Merck, Rahway, NJ 07065, USA. mirlinda_biba@merck.com
authors.forename: Mirlinda
authors.initials: M
authors.lastname: Biba
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Welch
authors.forename: Joe P
authors.initials: JP
authors.lastname: Foley
authors.forename: Bing
authors.initials: B
authors.lastname: Mao
authors.forename: Enrique
authors.initials: E
authors.lastname: Vazquez
authors.forename: Rebecca A
authors.initials: RA
authors.lastname: Arvary
mesh_terms: D056148:Chromatography, Reverse-Phase / Q000295:instrumentation / Q000379:methods*; D009841:Oligonucleotides / Q000032:analysis* / Q000737:chemistry*; D010316:Particle Size
chemical_list: D009841:Oligonucleotides