title: Antifungal activity of extracellular hydrolases produced by autolysing Aspergillus nidulans cultures.
abstract: Carbon-starving Aspergillus nidulans cultures produce high activities of versatile hydrolytic enzymes and, among these, ChiB endochitinase and EngA β-1,3-endoglucanase showed significant antifungal activity against various fungal species. Double deletion of engA and chiB diminished the antifungal activity of the fermentation broths and increased conidiogenesis and long-term viability of A. nidulans, but decreased the growth rate on culture media containing weak carbon sources. Production of ChiB and EngA can influence fungal communities either directly due to their antifungal properties or indirectly through their effects on vegetative growth. Our data suggest saprophytic fungi as promising future candidates to develop novel biocontrol technologies.
authors.affiliation: Department of Microbial Biotechnology and Cell Biology, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary.
authors.forename: Melinda
authors.initials: M
authors.lastname: Szilágyi
authors.forename: Fruzsina
authors.initials: F
authors.lastname: Anton
authors.forename: Katalin
authors.initials: K
authors.lastname: Forgács
authors.forename: Jae-Hyuk
authors.initials: JH
authors.lastname: Yu
authors.forename: István
authors.initials: I
authors.lastname: Pócsi
authors.forename: Tamás
authors.initials: T
authors.lastname: Emri
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D001233:Aspergillus nidulans / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D005110:Extracellular Space / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D005658:Fungi / Q000187:drug effects* / Q000254:growth & development; D015966:Gene Expression Regulation, Fungal; D006867:Hydrolases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D005656:Fungal Proteins; D006867:Hydrolases