title: Identification of chaperones in freeze tolerance in Saccharomyces cerevisiae.
abstract: Exposure to low temperatures reduces protein folding rates and induces the cold denaturation of proteins. Considering the roles played by chaperones in facilitating protein folding and preventing protein aggregation, chaperones must exist that confer tolerance to cold stress. Here, yeast strains lacking individual chaperones were screened for reduced freezing tolerance. In total, 19 of 82 chaperone-deleted strains tested were more sensitive to freeze-thaw treatment than wild-type cells. The reintroduction of the respective chaperone genes into the deletion mutants recovered the freeze tolerance. The freeze sensitivity of the chaperone-knockout strains was also retained in the presence of 20% glycerol.
authors.affiliation: Department of Molecular Biology, Sejong University, Seoul 143-747, Republic of Korea.
authors.forename: Mahendran Chinnamara
authors.initials: MC
authors.lastname: Naicker
authors.forename: I
authors.initials: I
authors.lastname: Seul Jo
authors.forename: Hana
authors.initials: H
authors.lastname: Im
mesh_terms: D005615:Freezing; D015966:Gene Expression Regulation, Fungal; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D018832:Molecular Chaperones; D029701:Saccharomyces cerevisiae Proteins