title: LARGE2 generates the same xylose- and glucuronic acid-containing glycan structures as LARGE.
abstract: LARGE (like-glycosyltransferase) and LARGE2 (glycosyltransferase-like 1B (GYLTL1B)) are homologous Golgi glycosyltransferases possessing two catalytic domains with homology to members of glycosyltransferase families GT8 and GT49. Mutations in human and mouse Large result in muscular dystrophy due to underglycosylation of dystroglycan. The systemic function of LARGE2 is unknown, but at a cellular level the enzyme can substitute for LARGE in glycosylating dystroglycan. Here, we show that LARGE2 catalyzes the same glycosylation reaction as LARGE. It is a bifunctional glycosyltransferase using uridine diphosphate (UDP)-xylose (Xyl) and UDP-glucuronic acid (GlcA) as donor sugars to produce a xyloglucuronan with alternating Xyl and GlcA residues.
authors.affiliation: Department of Cellular Chemistry,  Hannover Medical School, Carl-Neuberg-Strasse 1, D-30625 Hannover,  Germany.
authors.forename: Angel
authors.initials: A
authors.lastname: Ashikov
authors.forename: Falk F R
authors.initials: FF
authors.lastname: Buettner
authors.forename: Birgit
authors.initials: B
authors.lastname: Tiemann
authors.forename: Rita
authors.initials: R
authors.lastname: Gerardy-Schahn
authors.forename: Hans
authors.initials: H
authors.lastname: Bakker
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D006031:Glycosylation; D016695:Glycosyltransferases / Q000378:metabolism*; D051379:Mice; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism*; D014535:Uridine Diphosphate Glucuronic Acid / Q000378:metabolism*; D014540:Uridine Diphosphate Xylose / Q000378:metabolism*
chemical_list: D014535:Uridine Diphosphate Glucuronic Acid; D014540:Uridine Diphosphate Xylose; D016695:Glycosyltransferases; C502747:Large1 protein, mouse; C507407:Large2 protein, mouse; D017351:N-Acetylglucosaminyltransferases