title: Oligosaccharide structure determines prebiotic role of β-galactomannan against Salmonella enterica ser. Typhimurium in vitro.
abstract: Prebiotics and probiotics are considered natural alternatives to dietary antibiotics in animal production. Plant extracts and yeast cell walls are mannose-rich products that can be used as substrate for adhesion of Gram-negative bacteria. We assessed whether the structure of these saccharides is relevant to develop their role as prebiotics and therefore, their suitability to be used as alternatives to antibiotics to prevent intestinal infections in pigs. The prebiotic functionality of β-galactomannan (βGM), mannanoligosaccharide from yeast Saccharomyces cerevisiae (Mannan SC) and monosaccharide D-Mannose were studied in porcine intestinal epithelial cells (IPI-2I) challenged with Salmonella enterica ser. Typhimurium. Results showed that in vitro challenge with Salmonella induces the secretion of proinflammatory cytokine IL6 and chemokine CXCL8 compared with control without infection. Both βGM and Mannan SC, attenuate Salmonella-induced secretion of IL6 and CXCL8. Interestingly, cells treated with D-mannose showed similar levels of proinflammatory IL6 and CXCL8 compared with the control of infection. These data suggest that prebiotic role of βGM is related to its oligosaccharide structure.
authors.affiliation: Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Nutrició de Monogastrics, Constantí, Spain.
authors.forename: Roger
authors.initials: R
authors.lastname: Badia
authors.forename: Rosil
authors.initials: R
authors.lastname: Lizardo
authors.forename: Paz
authors.initials: P
authors.lastname: Martinez
authors.forename: Joaquim
authors.initials: J
authors.lastname: Brufau
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D004847:Epithelial Cells / Q000276:immunology / Q000382:microbiology; D005690:Galactose / Q000031:analogs & derivatives; D008351:Mannans / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D056692:Prebiotics; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000378:metabolism*; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents; D016207:Cytokines; D008351:Mannans; D009844:Oligosaccharides; D056692:Prebiotics; C012990:galactomannan; D005690:Galactose
keywords: Saccharomyces cerevisiae; SalmonellaTyphimurium; intestinal epithelial cells; mannanoligosaccharides