title: Alterations in DRBD3 ribonucleoprotein complexes in response to stress in Trypanosoma brucei.
abstract: Regulation of RNA polymerase II transcription initiation is apparently absent in trypanosomes. Instead, these eukaryotes control gene expression mainly at the post-transcriptional level. Regulation is exerted through the action of numerous RNA-binding proteins that modulate mRNA processing, turnover, translation and localization. In this work we show that the RNA-binding protein DRBD3 resides in the cytoplasm, but localizes to the nucleus upon oxidative challenge and to stress granules under starvation conditions. DRBD3 associates with other proteins to form a complex, the composition of which is altered by cellular stress. Interestingly, target mRNAs remain bound to DRBD3 under stress conditions. Our results suggest that DRBD3 transports regulated mRNAs within the cell in the form of ribonucleoprotein complexes that are remodeled in response to environmental cues.
authors.affiliation: Instituto de Parasitología y Biomedicina "López-Neyra", IPBLN-CSIC, Armilla, Granada, Spain.
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Fernández-Moya
authors.forename: Angélica
authors.initials: A
authors.lastname: García-Pérez
authors.forename: Susanne
authors.initials: S
authors.lastname: Kramer
authors.forename: Mark
authors.initials: M
authors.lastname: Carrington
authors.forename: Antonio M
authors.initials: AM
authors.lastname: Estévez
mesh_terms: D018053:Arsenites / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D005786:Gene Expression Regulation; D018384:Oxidative Stress; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D014346:Trypanosoma brucei brucei / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
chemical_list: D018053:Arsenites; D015800:Protozoan Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C015001:arsenite