title: Sensitive and simultaneous detection of different disease markers using multiplexed gold nanorods.
abstract: A multiplexed bioanalytical assay is produced by incorporating two types of gold nanorods (GNRs). Besides retaining the desirable features of common GNRs LSPR sensors, this sensor is easy to fabricate and requires only a visible-NIR spectrometer for detection. This assay can simultaneously detect different acceptor-ligand pairs by choosing the proper GNRs possessing various LPWs in a wide detection wavelength range and can be developed into a high-throughput detection method. This bioanalytical assay allows easy detection of human serum specimens infected by S. japonicum and tuberculosis (TB) from human serum specimens (human serum/Tris-HCl buffer ratio=1:10(4)) without the need for sample pretreatment. The technique is very sensitive compared to other standard methods such as indirect hemagglutination assays (IHA) that require a serum concentration ratio of larger than 1:20 and enzyme-linked immunosorbent assays (ELISA) requiring a ratio larger than 1:100. This methodology can be readily extended to other immunoassays to realize wider diagnostic applications.
authors.affiliation: Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, China. hhwn09@163.com
authors.forename: Haowen
authors.initials: H
authors.lastname: Huang
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Shaowen
authors.initials: S
authors.lastname: Huang
authors.forename: Shishan
authors.initials: S
authors.lastname: Yuan
authors.forename: Bo
authors.initials: B
authors.lastname: Liao
authors.forename: Shoujun
authors.initials: S
authors.lastname: Yi
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Zeng
authors.forename: Paul K
authors.initials: PK
authors.lastname: Chu
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay; D008024:Ligands; D046529:Microscopy, Electron, Transmission; D043942:Nanotubes / Q000737:chemistry*; D012015:Reference Standards; D012549:Schistosoma japonicum / Q000502:physiology; D012554:Schistosomiasis japonica / Q000097:blood / Q000175:diagnosis; D012680:Sensitivity and Specificity; D013997:Time Factors; D014376:Tuberculosis / Q000097:blood / Q000175:diagnosis
chemical_list: D015415:Biomarkers; D008024:Ligands; D006046:Gold