title: Single-molecule super-resolution imaging in bacteria.
abstract: Bacteria have evolved complex, multi-component cellular machineries to carry out fundamental cellular processes such as cell division/separation, locomotion, protein secretion, DNA transcription/replication, or conjugation/competence. Diffraction of light has so far restricted the use of conventional fluorescence microscopy to reveal the composition, internal architecture and dynamics of these important machineries. This review describes some of the more recent advances on single-molecule super-resolution microscopy methods applied to bacteria and highlights their application to chemotaxis, cell division, DNA segregation, and DNA transcription machineries. Finally, we discuss some of the lessons learned from this approach, and future perspectives.
authors.affiliation: Centre de Biochimie Structurale, CNRS UMR5048, INSERM U554, Université de Montpellier, 29 rue de Navacelles, 34090 Montpellier, France.
authors.forename: D I
authors.initials: DI
authors.lastname: Cattoni
authors.forename: J B
authors.initials: JB
authors.lastname: Fiche
authors.forename: M
authors.initials: M
authors.lastname: Nöllmann
mesh_terms: D001419:Bacteria / Q000737:chemistry*; D001426:Bacterial Proteins / Q000032:analysis*; D008853:Microscopy / Q000379:methods*; D046912:Multiprotein Complexes / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D046912:Multiprotein Complexes