title: Development of a micro-imaging probe for functional brain imaging.
abstract: Multicellular neuronal activities should be investigated to reveal the dynamics of the neuronal circuit. Optical recording from neuronal populations is suitable for recording multicellular activities. We fabricated the prototype of the micro-imaging probe in combination with a gradient index lens and image fiber. This probe has a smaller diameter than traditional probes. We found an optimal optical configuration for maximizing the efficiency of the imaging probe. Using this optical configuration with the prototype of the imaging probe, the fluorescence images were captured from neurons expressing green fluorescent protein in a cerebellar block preparation, and the calcium-dependent images were sampled in a mouse brain slice preparation. Our optical system would facilitate the in vivo imaging studies with less invasive manners using thinner optic fiber than previously made.
authors.affiliation: Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan. osanai@med.tohoku.ac.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Osanai
authors.forename: Taro
authors.initials: T
authors.lastname: Suzuki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Tamura
authors.forename: Tsugio
authors.initials: T
authors.lastname: Yonemura
authors.forename: Issei
authors.initials: I
authors.lastname: Mori
authors.forename: Yuchio
authors.initials: Y
authors.lastname: Yanagawa
authors.forename: Hiromu
authors.initials: H
authors.lastname: Yawo
authors.forename: Hajime
authors.initials: H
authors.lastname: Mushiake
mesh_terms: D000818:Animals; D001921:Brain; D005336:Fiber Optic Technology / Q000295:instrumentation* / Q000379:methods; D051379:Mice; D008904:Miniaturization / Q000295:instrumentation; D059906:Neuroimaging / Q000295:instrumentation* / Q000379:methods