title: Transcript profiling of microRNAs during the early development of the maize brace root via Solexa sequencing.
abstract: To characterize the microRNAs that contribute to the development of brace root, Solexa high-throughput sequencing of three libraries derived from tissues of node (N), nodes with just-emerged brace roots (NR), and nodes with just-emerged brace roots after IAA treatment (NRI) was performed. Total 650,793, 957,303 and 1,082,948 genome-matched unique reads were obtained in N, NR and NRI libraries, respectively. Further analysis confirmed the authenticity of 137 known miRNAs and the discovery of 159 novel miRNAs in maize. 14 conserved and 16 novel miRNAs differentially expressed in brace root, as well as 15 target genes, were identified and validated by qRT-PCR during maize brace root development. Moreover, we identified 9 miRNA precursor-matched novel sRNAs that may form miRNA clusters, as well as 24 nt siRNAs in the three libraries. In addition, we suggest that auxin represent a regulator in brace root development and can be regulated at the posttranscriptional level by miRNAs.
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Kang
authors.initials: K
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Yun-xue
authors.initials: YX
authors.lastname: Lei
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Yue-min
authors.initials: YM
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Jin-guang
authors.initials: JG
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China. Electronic address: cawu@sdau.edu.cn.
authors.forename: Chang-Ai
authors.initials: CA
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China. Electronic address: cczheng@sdau.edu.cn.
authors.forename: Cheng-Chao
authors.initials: CC
authors.lastname: Zheng
mesh_terms: D001483:Base Sequence; D020869:Gene Expression Profiling; D015723:Gene Library; D007210:Indoleacetic Acids / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics*; D017423:Sequence Analysis, RNA; D003313:Zea mays / Q000235:genetics*
chemical_list: D007210:Indoleacetic Acids; D035683:MicroRNAs; D018749:RNA, Plant