title: Fluorescence laser microdissection reveals a distinct pattern of gene activation in the mouse hippocampal region.
abstract: A histoanatomical context is imperative in an analysis of gene expression in a cell in a tissue to elucidate physiological function of the cell. In this study, we made technical advances in fluorescence laser microdissection (LMD) in combination with the absolute quantification of small amounts of mRNAs from a region of interest (ROI) in fluorescence-labeled tissue sections. We demonstrate that our fluorescence LMD-RTqPCR method has three orders of dynamic range, with the lower limit of ROI-size corresponding to a single cell. The absolute quantification of the expression levels of the immediate early genes in an ROI equivalent to a few hundred neurons in the hippocampus revealed that mice transferred from their home cage to a novel environment have distinct activation profiles in the hippocampal regions (CA1, CA3, and DG) and that the gene expression pattern in CA1, but not in the other regions, follows a power law distribution.
authors.affiliation: Laboratory of Environmental Health Sciences, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Yoshioka
authors.forename: Nozomi
authors.initials: N
authors.lastname: Endo
authors.forename: Akie
authors.initials: A
authors.lastname: Kurashige
authors.forename: Asahi
authors.initials: A
authors.lastname: Haijima
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Endo
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Shibata
authors.forename: Ryutaro
authors.initials: R
authors.lastname: Nishiyama
authors.forename: Masaki
authors.initials: M
authors.lastname: Kakeyama
authors.forename: Chiharu
authors.initials: C
authors.lastname: Tohyama
mesh_terms: D000818:Animals; D056547:CA1 Region, Hippocampal / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D056654:CA3 Region, Hippocampal / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D018891:Dentate Gyrus / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D005260:Female; D005453:Fluorescence; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D018014:Gene Transfer Techniques; D006624:Hippocampus / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D007834:Lasers; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D042282:Microdissection / Q000379:methods*; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D011247:Pregnancy; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015533:Transcriptional Activation; D000097573:Red Fluorescent Protein
chemical_list: D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein