title: Effects of fluid shear stress on the expression of Omi/HtrA2 in human umbilical vein endothelial cells.
abstract: To investigate the molecular mechanisms of laminar shear stress on the inhibition of apoptosis in endothelial cells, human umbilical vein endothelial cells (HUVECs) were starved in medium containing 2% fetal bovine serum (FBS) and treated with 15 dyne/cm2 shear stress. We confirmed that 15 dyne/cm2 shear stress inhibited the expression of Omi/HtrA2 at the mRNA and protein levels in cultured HUVECs. Furthermore, the release of Omi/HtrA2 from the mitochondria was induced by removal of basic fibroblast growth factor and decrease of FBS in the medium, while shear stress inhibited its release under the same conditions. These results suggest that downregulation of Omi/HtrA2 may contribute to the potent anti-atherosclerotic effect of shear stress by preventing endothelial cells from entering apoptosis.
authors.affiliation: Department of Pharmacology, School of Medicine, Xiamen University, Xiamen 361005, Fujian, PR China.
authors.forename: Liang-Liang
authors.initials: LL
authors.lastname: Sun
authors.forename: Le
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiang-Lan
authors.initials: XL
authors.lastname: Meng
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
mesh_terms: D017209:Apoptosis / Q000235:genetics; D005786:Gene Expression Regulation; D000074923:High-Temperature Requirement A Serine Peptidase 2; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism*; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D021381:Protein Transport; D012697:Serine Endopeptidases / Q000235:genetics* / Q000378:metabolism; D033081:Shear Strength; D013314:Stress, Mechanical
chemical_list: D024101:Mitochondrial Proteins; D012697:Serine Endopeptidases; C000615914:HTRA2 protein, human; D000074923:High-Temperature Requirement A Serine Peptidase 2