title: Short communication: neutralizing antibodies in HIV-1-infected Brazilian individuals.
abstract: Tests for the detection of the humoral immune response to HIV-1 have to be standardized and established, demanding regional efforts. For this purpose the neutralizing antibody (NAb) assay for HIV-1 in TZM-bl cells was introduced in Brazil. Twenty plasma samples from HIV-1-infected individuals were assayed: 10 progressors and 10 long-term nonprogressors. These were tested against eight env-pseudotyped viruses (psVs) in the TZM-bl NAb assay and against HIV-1 strain HTLV/IIIB (HIV-1 IIIB) in primary lymphocytes. Forty-four percent of the samples showed neutralizing titers for psVs and 55% for HIV-1 IIIB. Plasma from progressors showed a broader neutralization and a higher potency. The introduction of these reference reagents encourages the participation of Brazil in future comparative assessments of anti-HIV-1 antibodies.
authors.affiliation: AIDS and Molecular Immunology Laboratory, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil. dalziza@gmail.com
authors.forename: Dalziza Victalina
authors.initials: DV
authors.lastname: Almeida
authors.forename: Mariza Gonçalvez
authors.initials: MG
authors.lastname: Morgado
authors.forename: Fernanda Heloise
authors.initials: FH
authors.lastname: Côrtes
authors.forename: Monick Lindermeyer
authors.initials: ML
authors.lastname: Guimarães
authors.forename: Leila
authors.initials: L
authors.lastname: Mendonça-Lima
authors.forename: Jose Henrique
authors.initials: JH
authors.lastname: Pilotto
authors.forename: Beatriz
authors.initials: B
authors.lastname: Grinsztejn
authors.forename: Valdiléa Gonçalves
authors.initials: VG
authors.lastname: Veloso
authors.forename: Vera
authors.initials: V
authors.lastname: Bongertz
mesh_terms: D057134:Antibodies, Neutralizing / Q000097:blood*; D001938:Brazil; D005260:Female; D015483:HIV Antibodies / Q000097:blood*; D015658:HIV Infections / Q000276:immunology* / Q000821:virology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008297:Male; D009500:Neutralization Tests
chemical_list: D057134:Antibodies, Neutralizing; D015483:HIV Antibodies