title: Antimicrobial activity of bifidobacterial bacteriocin-like substances.
abstract: Antagonistic activity of 13 bifidobacterial strains, isolated from humans, has been studied. It was shown that specific antagonistic activity in bifidobacteria is a strain characteristic and does not depend on species of these microorganisms. It was determined that bifidobacteria are able to produce bacteriocin-like substances against both gram-positive and gram-negative bacteria. Strains Bifidobacterium sp. 278 and B. bifidum 174 produced antimicrobial substances of wide spectrum of activity and manifested the highest antagonistic activity as compared to the rest of bifidobacterial strains studied. The maximal activity of bacteriocin production by strains B. bifidum 174 ma Bifidobacterium sp. 278 occurs between 8-16 hours of cultivation that is in the late logarithmic phase of growth. According to obtained characteristics the antimicrobial substances are complex peptides and belong to the 4th class of bacteriocins.
authors.affiliation: Zabolotny Institute of Microbiology and Virology, NAS of Ukraine 154 Zabolotny St., Kyiv, MSP, D03680, Ukraine.
authors.forename: O A
authors.initials: OA
authors.lastname: Poltavska
authors.forename: N K
authors.initials: NK
authors.lastname: Kovalenko
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000898:Antibiosis; D001430:Bacteriocins / Q000737:chemistry / Q000494:pharmacology*; D001644:Bifidobacterium / Q000166:cytology / Q000302:isolation & purification / Q000472:pathogenicity*; D002675:Child, Preschool; D017077:Culture Media, Conditioned / Q000302:isolation & purification / Q000494:pharmacology; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000254:growth & development; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D007223:Infant; D008297:Male; D008826:Microbial Sensitivity Tests; D013045:Species Specificity; D013997:Time Factors
chemical_list: D001430:Bacteriocins; D017077:Culture Media, Conditioned