title: Matrix metalloproteinases and vascular endothelial growth factor expression in canine leukaemias.
abstract: Matrix metalloproteinases (MMPs) and vascular endothelial growth factor (VEGF) play a coordinated role during neoplastic invasion and proliferation. VEGF and MMPs expression was investigated in canine leukaemias by immunocytochemistry (MMP-9, MMP-2, VEGF-A) and quantitative RT-PCR (MMP-2, MMP-9, MT1-MMP, TIMP-1, TIMP-2, RECK, VEGF-A, VEGF-164). Blood samples were obtained from dogs with acute leukaemia (AL; n = 11) and chronic lymphocytic leukaemia (CLL; n = 12). Levels of MMP-9, TIMP-1 and VEGF-A were higher in CLL than AL and controls. Expression of TIMP-2 and MT1-MMP mRNA was significantly higher in AL than CLL. Significant positive correlations were found between MMP-9 and TIMP-1 and between MMP-9 and VEGF-A in CLL. These results suggest a potential role of MMP-9, MT1-MMP, TIMP-1, TIMP-2 and VEGF in tissue migration and angiogenesis in canine leukaemia.
authors.affiliation: Department of Comparative Biomedicine and Food Science, University of Padova, Viale dell'Università 16, 35020 Agripolis Legnaro (PD), Italy.
authors.forename: Arianna
authors.initials: A
authors.lastname: Aricò
authors.forename: Mery
authors.initials: M
authors.lastname: Giantin
authors.forename: Mariaelena
authors.initials: M
authors.lastname: Gelain
authors.forename: Fulvio
authors.initials: F
authors.lastname: Riondato
authors.forename: Michele
authors.initials: M
authors.lastname: Mortarino
authors.forename: Stefano
authors.initials: S
authors.lastname: Comazzi
authors.forename: Mauro
authors.initials: M
authors.lastname: Dacasto
authors.forename: Massimo
authors.initials: M
authors.lastname: Castagnaro
authors.forename: Luca
authors.initials: L
authors.lastname: Aresu
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D007150:Immunohistochemistry / Q000662:veterinary; D015456:Leukemia, Biphenotypic, Acute / Q000378:metabolism / Q000662:veterinary*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism / Q000662:veterinary*; D008297:Male; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
chemical_list: D042461:Vascular Endothelial Growth Factor A; D020782:Matrix Metalloproteinases