title: Construction of a peptide microarray for auto-anti- body detection.
abstract: Peptide and protein microarrays provide a multiplex approach to identification and quantification of protein-protein interactions (PPI), useful to study for instance antigen-antibody properties. Multivariate serology assays detecting multiple tumor auto-antibodies (TAA)  is an emerging class of blood tests for cancer detection. Here we describe the efficient coupling of peptide baits derived from the BRCA1-associated RING domain protein 1 (BARD1) to a solid surface and detection of a commercially available anti-BARD1 antibody with this newly designed peptide  microarray. Analytical sensitivity and specificity were shown to be comparable to a microtiter plate based enzyme-linked immunosorbent assay (ELISA).
authors.affiliation: HES-SO Valais, Institute of Life Technologies, Route du Rawyl 47, CH-1950 Sion 2, Switzerland.
authors.forename: Vincent
authors.initials: V
authors.lastname: Cosandey
authors.forename: Fabien
authors.initials: F
authors.lastname: Debrot
authors.forename: Jérémy
authors.initials: J
authors.lastname: Kaeser
authors.forename: Roger
authors.initials: R
authors.lastname: Marti
authors.forename: Philippe
authors.initials: P
authors.lastname: Passeraub
authors.forename: Jannick
authors.initials: J
authors.lastname: Pétremand
authors.forename: Denis
authors.initials: D
authors.lastname: Prim
authors.forename: Marc E
authors.initials: ME
authors.lastname: Pfeifer
mesh_terms: D000912:Antibodies, Neoplasm / Q000032:analysis*; D057930:Click Chemistry / Q000379:methods; D006801:Humans; D008856:Microscopy, Fluorescence; D040081:Protein Array Analysis / Q000379:methods*; D025521:Tumor Suppressor Proteins / Q000737:chemistry*; D044767:Ubiquitin-Protein Ligases / Q000737:chemistry*
chemical_list: D000912:Antibodies, Neoplasm; D025521:Tumor Suppressor Proteins; C102449:BARD1 protein, human; D044767:Ubiquitin-Protein Ligases