title: Biodegradation of chlorpyrifos and 3,5,6-trichloro-2-pyridinol by Cupriavidus sp. DT-1.
abstract: A bacterial strain, Cupriavidus sp. DT-1, capable of degrading chlorpyrifos and 3,5,6-trichloro-2-pyridinol (TCP) and using these compounds as sole carbon source was isolated and characterized. Investigation of the degradation pathway showed that chlorpyrifos was first hydrolyzed to TCP, successively dechlorinated to 2-pyridinol, and then subjected to the cleavage of the pyridine ring and further degradation. The mpd gene, encoding the enzyme responsible for chlorpyrifos hydrolysis to TCP, was cloned and expressed in Escherichia coli BL21. Inoculation of chlorpyrifos-contaminated soil with strain DT-1 resulted in a degradation rate of chlorpyrifos and TCP of 100% and 94.3%, respectively as compared to a rate of 28.2% and 19.9% in uninoculated soil. This finding suggests that strain DT-1 has potential for use in bioremediation of chlorpyrifos-contaminated environments.
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agriculture University, Nanjing 210095, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Lu
authors.forename: Qinfen
authors.initials: Q
authors.lastname: Li
authors.forename: Hongming
authors.initials: H
authors.lastname: Liu
authors.forename: Zhaozhong
authors.initials: Z
authors.lastname: Feng
authors.forename: Xin
authors.initials: X
authors.lastname: Yan
authors.forename: Qing
authors.initials: Q
authors.lastname: Hong
authors.forename: Shunpeng
authors.initials: S
authors.lastname: Li
mesh_terms: D043303:Aryldialkylphosphatase / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001673:Biodegradation, Environmental; D019149:Bioreactors; D004390:Chlorpyrifos / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D049921:Cupriavidus / Q000201:enzymology* / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004926:Escherichia coli; D005798:Genes, Bacterial; D007700:Kinetics; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D011728:Pyridones / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D011728:Pyridones; D012989:Soil Pollutants; C012587:3,5,6-trichloro-2-pyridinol; D043303:Aryldialkylphosphatase; D004390:Chlorpyrifos