title: The expression and functional characterization associated with cell             apoptosis and proteomic analysis of the novel gene MLAA-34 in U937 cells.
abstract: MLAA-34 is a novel acute monocytic leukemia (M5)-associated antigen (MLAA)             that plays a role in the apoptosis of U937 cells. However, the expression and             molecular mechanism of MLAA-34 in U937 cells remain largely unclear. Here, we             utilized three strategies to gain insight into the expression and molecular functions             of MLAA-34 and to identify its interacting proteins and pathways involved in the             fine-tuning of the MLAA-34 response. Western blot analysis was performed to assess             the expression of MLAA-34 in 41 cell lines and five mixed cell types, which revealed             that MLAA-34 is most strongly expressed in U937 cells. Immunostaining indicated             that MLAA-34 is localized in the cytoplasm and cell membrane. Furthermore, lentivirus-mediated             overexpression of MLAA-34 in the U937 cell line led to significant suppression             of apoptosis and increased the potential of tumorigenicity. Co-immunoprecipitation             (Co-IP), shotgun and bioinformatic analysis identified 256 proteins and 225 of             them were annotated by gene ontology categories. This analysis revealed 71 proteins             involved in cell apoptosis or proliferation of biological processes and signaling             pathways. Moreover, the effect of MLAA-34 apoptosis may be through interaction             with the Ras, Wnt, calcium and chemokine signaling pathways and thirteen of the             annotated proteins may interact with MLAA-34 and participate in carcinogenesis             directly. This study provides a basis for a better understanding of the molecular             mechanism and proteomics in the inhibition of apoptosis by MLAA-34 in U937 cells             and indicates that MLAA-34 may be a potential candidate for the early diagnosis             and therapeutic application of M5.
authors.affiliation: Department of Clinical Hematology, Affiliated No. 2 Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710004, PR China.
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Zhang
authors.forename: Wang-Gang
authors.initials: WG
authors.lastname: Zhang
authors.forename: Peng-Yu
authors.initials: PY
authors.lastname: Zhang
authors.forename: Xing-Mei
authors.initials: XM
authors.lastname: Cao
authors.forename: Ai-Li
authors.initials: AL
authors.lastname: He
authors.forename: Yin-Xia
authors.initials: YX
authors.lastname: Chen
authors.forename: Liu-Fang
authors.initials: LF
authors.lastname: Gu
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics* / Q000378:metabolism*; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism*; D002462:Cell Membrane; D049109:Cell Proliferation; D003593:Cytoplasm; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D010735:Phosphoglycerate Kinase / Q000378:metabolism; D040901:Proteomics; D015398:Signal Transduction / Q000235:genetics; D014162:Transfection; D020298:U937 Cells; D015854:Up-Regulation / Q000235:genetics; D051176:beta Catenin / Q000378:metabolism; D020692:rab3 GTP-Binding Proteins / Q000378:metabolism; D020668:rap GTP-Binding Proteins / Q000378:metabolism
chemical_list: D000951:Antigens, Neoplasm; D051017:Apoptosis Regulatory Proteins; C534238:CAB39L protein, human; D051176:beta Catenin; C577282:PGK1 protein, human; D010735:Phosphoglycerate Kinase; C492134:RAB3D protein, human; C116753:RAP1B protein, human; D020692:rab3 GTP-Binding Proteins; D020668:rap GTP-Binding Proteins