title: Solution structure of the cold-shock-like protein from Rickettsia rickettsii.
abstract: Rocky Mountain spotted fever is caused by Rickettsia rickettsii infection. R. rickettsii can be transmitted to mammals, including humans, through the bite of an infected hard-bodied tick of the family Ixodidae. Since the R. rickettsii genome contains only one cold-shock-like protein and given the essential nature of cold-shock proteins in other bacteria, the structure of the cold-shock-like protein from R. rickettsii was investigated. With the exception of a short α-helix found between β-strands 3 and 4, the solution structure of the R. rickettsii cold-shock-like protein has the typical Greek-key five-stranded β-barrel structure found in most cold-shock domains. Additionally, the R. rickettsii cold-shock-like protein, with a ΔG of unfolding of 18.4 kJ mol(-1), has a similar stability when compared with other bacterial cold-shock proteins.
authors.affiliation: Department of Chemistry, University of Wisconsin-Whitewater, 800 West Main Street, Whitewater, WI 53190, USA.
authors.forename: Kyle P
authors.initials: KP
authors.lastname: Gerarden
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Fuchs
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Koch
authors.forename: Melissa M
authors.initials: MM
authors.lastname: Mueller
authors.forename: David R
authors.initials: DR
authors.lastname: Graupner
authors.forename: Justin T
authors.initials: JT
authors.lastname: O'Rorke
authors.forename: Caleb D
authors.initials: CD
authors.lastname: Frost
authors.forename: Heather A
authors.initials: HA
authors.lastname: Heinen
authors.forename: Emily R
authors.initials: ER
authors.lastname: Lackner
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Schoeller
authors.forename: Paul G
authors.initials: PG
authors.lastname: House
authors.forename: Francis C
authors.initials: FC
authors.lastname: Peterson
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Veldkamp
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D057773:Cold Shock Proteins and Peptides / Q000737:chemistry*; D017124:Conserved Sequence; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D055550:Protein Stability; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D058767:Protein Unfolding; D012284:Rickettsia rickettsii; D013816:Thermodynamics
chemical_list: D001426:Bacterial Proteins; D057773:Cold Shock Proteins and Peptides