title: Osteoblast, fibroblast and in vivo biological response to poly(vinylidene fluoride) based composite materials.
abstract: Electroactive materials can be taken to advantage for the development of sensors and actuators as well as for novel tissue engineering strategies. Composites based on poly(vinylidene fluoride), PVDF, have been evaluated with respect to their biological response. Cell viability and proliferation were performed in vitro both with Mesenchymal Stem Cells differentiated to osteoblasts and Human Fibroblast Foreskin 1. In vivo tests were also performed using 6-week-old C57Bl/6 mice. It was concluded that zeolite and clay composites are biocompatible materials promoting cell response and not showing in vivo pro-inflammatory effects which renders both of them attractive for biological applications and tissue engineering, opening interesting perspectives to development of scaffolds from these composites. Ferrite and silver nanoparticle composites decrease osteoblast cell viability and carbon nanotubes decrease fibroblast viability. Further, carbon nanotube composites result in a significant increase in local vascularization accompanied an increase of inflammatory markers after implantation.
authors.affiliation: Department of Biochemistry (U38-FCT), Faculty of Medicine, University of Porto, 4200-319, Porto, Portugal.
authors.forename: R
authors.initials: R
authors.lastname: Costa
authors.forename: C
authors.initials: C
authors.lastname: Ribeiro
authors.forename: A C
authors.initials: AC
authors.lastname: Lopes
authors.forename: P
authors.initials: P
authors.lastname: Martins
authors.forename: V
authors.initials: V
authors.lastname: Sencadas
authors.forename: R
authors.initials: R
authors.lastname: Soares
authors.forename: S
authors.initials: S
authors.lastname: Lanceros-Mendez
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D011145:Polyvinyls / Q000737:chemistry* / Q000494:pharmacology; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry; D005465:Fluorocarbon Polymers
chemical_list: D011108:Polymers; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers