title: [Differential expression genes of bone tissues surrounding implants in diabetic rats by gene chip].
abstract: OBJECTIVE
  To compare mRNA expression profiles of bone tissues surrounding implants between normal rats and rats with diabetes using microarray technology.
  
  
  METHODS
  Six Wistar rats were randomly selected and divided into normal model group and diabetic group. Diabetic model condition was established by injecting Streptozotocin into peritoneal space. Titanium implants were implanted into the epiphyseal end of the rats' tibia. Bone tissues surrounding implant were harvested and sampled after 3 months to perform comprehensive RNA gene expression profiling, including 17983 for genome-wide association study.GO analysis was used to compare different gene expression and real-time PCR was used to confirm the results on core samples.
  
  
  RESULTS
  The results indicated that there were 1084 differential gene expression. In the diabetic model, there were 352 enhanced expression genes, 732 suppressed expression genes. GO analysis involved 1154 different functional type.
  
  
  CONCLUSIONS
  Osteoblast related gene expressions in bone tissue samples of diabetic rats were decreased, and lipid metabolism pathway related gene expression was increased.
authors.affiliation: Shandong Provincial Key Laboratory of Oral Biomedicine, Shandong Province, China. xinxin1986815918@yahoo.com.cn
authors.forename: Xin-xin
authors.initials: XX
authors.lastname: Wang
authors.forename: Yue
authors.initials: Y
authors.lastname: Ma
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Bao-qi
authors.initials: BQ
authors.lastname: Jiang
authors.forename: Jing
authors.initials: J
authors.lastname: Lan
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental; D055106:Genome-Wide Association Study; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts; D051381:Rats; D017208:Rats, Wistar; D013977:Tibia; D014025:Titanium
chemical_list: D014025:Titanium