title: Naringenin degradation by the endophytic diazotroph Herbaspirillum seropedicae SmR1.
abstract: Several bacteria are able to degrade flavonoids either to use them as carbon sources or as a detoxification mechanism. Degradation pathways have been proposed for several bacteria, but the genes responsible are not known. We identified in the genome of the endophyte Herbaspirillum seropedicae SmR1 an operon potentially associated with the degradation of aromatic compounds. We show that this operon is involved in naringenin degradation and that its expression is induced by naringenin and chrysin, two closely related flavonoids. Mutation of fdeA, the first gene of the operon, and fdeR, its transcriptional activator, abolished the ability of H. seropedicae to degrade naringenin.
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná, CP 19046, 81531-980, Curitiba, PR, Brazil.
authors.forename: A M
authors.initials: AM
authors.lastname: Marin
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná, CP 19046, 81531-980, Curitiba, PR, Brazil.
authors.forename: E M
authors.initials: EM
authors.lastname: Souza
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná, CP 19046, 81531-980, Curitiba, PR, Brazil.
authors.forename: F O
authors.initials: FO
authors.lastname: Pedrosa
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná, CP 19046, 81531-980, Curitiba, PR, Brazil.
authors.forename: L M
authors.initials: LM
authors.lastname: Souza
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná, CP 19046, 81531-980, Curitiba, PR, Brazil.
authors.forename: G L
authors.initials: GL
authors.lastname: Sassaki
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná, CP 19046, 81531-980, Curitiba, PR, Brazil.
authors.forename: V A
authors.initials: VA
authors.lastname: Baura
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná, CP 19046, 81531-980, Curitiba, PR, Brazil.
authors.forename: M G
authors.initials: MG
authors.lastname: Yates
authors.affiliation: Department of Genetics, Universidade Federal do Paraná, CP 19071, 81531-980, Curitiba, PR, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Wassem
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná, CP 19046, 81531-980, Curitiba, PR, Brazil.
authors.forename: R A
authors.initials: RA
authors.lastname: Monteiro
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001711:Biotransformation; D044950:Flavanones / Q000378:metabolism*; D005419:Flavonoids / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D055786:Gene Knockout Techniques; D042803:Herbaspirillum / Q000235:genetics / Q000378:metabolism*; D009876:Operon
chemical_list: D001426:Bacterial Proteins; D044950:Flavanones; D005419:Flavonoids; C043561:chrysin; C005273:naringenin