title: Antiproliferative and immunoactivatory ability of an enzymatic extract from rice bran.
abstract: The validation of natural products as source of functional foods or nutraceuticals has become an important issue in current health research. Thus, the present work has tested on MOLT-4 cells (human T cell acute lymphoblastic leukemic) the antiproliferative effect of a water-soluble enzymatic extract from rice bran (EERB). Present work shows that EERB induces cellular death in MOLT-4 cells in a dose-dependent way (0-10mg/mL) but not in non-tumoral lymphocytes. Flow cytometric analysis of MOLT-4 cells treated with EERB showed the presence of death cells by apoptosis rather than necrosis. Additionally, EERB also exerts an immunoactivatory effect on N13 microglia cells, by inducing TNF-alpha (tumour necrosis factor-α) expression, which plays a key role in the innate immune response to infection. Accordingly, we can propose EERB as a useful natural standardized extract with antiproliferative and immunoactivatory ability that would be beneficial to apply in the functional food field.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Universidad de Sevilla, c/Profesor García González, 2, 41012 Sevilla, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Revilla
authors.forename: C
authors.initials: C
authors.lastname: Santa-María
authors.forename: E
authors.initials: E
authors.lastname: Miramontes
authors.forename: M
authors.initials: M
authors.lastname: Candiracci
authors.forename: B
authors.initials: B
authors.lastname: Rodríguez-Morgado
authors.forename: M
authors.initials: M
authors.lastname: Carballo
authors.forename: J
authors.initials: J
authors.lastname: Bautista
authors.forename: A
authors.initials: A
authors.lastname: Castaño
authors.forename: J
authors.initials: J
authors.lastname: Parrado
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D006131:Growth Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007155:Immunologic Factors / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D012275:Oryza / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology
chemical_list: D006131:Growth Inhibitors; D007155:Immunologic Factors; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D010447:Peptide Hydrolases