title: Salinicola zeshunii sp. nov., a moderately halophilic bacterium isolated from soil of a chicken farm.
abstract: The taxonomic status of a moderately halophilic bacterium, strain N4(T), isolated from soil of a chicken farm in China was determined. It was Gram-negative, non-spore-forming, motile, and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequence indicated that this strain belonged to the genus Salinicola, as it showed the highest sequence similarities to Salinicola salaries M27(T) (98.3 %), Salinicola socius SMB35(T) (98.1 %), and Salinicola halophilus CG4.1(T) (98.1 %). The major cellular fatty acids were C(16:0) (25.6 %), C(18:1)ω7c (35.0 %), and C(19:0) cyclo ω8c (11.9 %), which are properties shared by members of the genus Salinicola. The DNA G+C content of strain N4(T) was 69.1 mol %. The level of DNA-DNA relatedness between strain N4(T) and the other three type strains of the genus of Salinicola salaries M27(T), Salinicola socius SMB35(T), and Salinicola halophilus CG4.1(T) were 34.3, 28.7, and 26.9 %, respectively. Based on the results of phenotypic, chemotaxonomic, DNA-DNA relatedness, and phylogenetic analysis, strain N4(T) should be classified as a novel species of the genus Salinicola, for which the name Salinicola zeshunii sp. nov. is proposed, with strain N4(T) (=KACC 16602(T) = CCTCC AB 2012912(T)) as the type strain.
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Li
authors.initials: L
authors.lastname: Cao
authors.forename: Qiuxiang
authors.initials: Q
authors.lastname: Yan
authors.forename: Haiyan
authors.initials: H
authors.lastname: Ni
authors.forename: Gang
authors.initials: G
authors.lastname: Hu
authors.forename: Qing
authors.initials: Q
authors.lastname: Hong
authors.forename: Shunpeng
authors.initials: S
authors.lastname: Li
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002645:Chickens; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D044129:Halomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S