title: Technologies for systems-level analysis of specific cell types in plants.
abstract: The study of biological processes at cell type resolution requires the isolation of the specific cell types from an organism, but this presents a great technical challenge. In recent years a number of methods have been developed that allow deep analyses of the epigenome, transcriptome, and ribosome-associated mRNA populations in individual cell types. The application of these methods has lead to a clearer understanding of important issues in plant biology, including cell fate specification and cell type-specific responses to the environment. In this review, we discuss current mechanical- and affinity-based technologies available for isolation and analysis of individual cell types in a plant. The integration of these methods is proposed as a means of achieving a holistic view of cellular processes at all levels, from chromatin dynamics to metabolomics. Finally, we explore the limitations of current methods and the needs for future technological development.
authors.affiliation: Department of Biology, Emory University, Atlanta, GA 30322, USA.
authors.forename: Dongxue
authors.initials: D
authors.lastname: Wang
authors.affiliation: Graduate program in Genetics and Molecular Biology of the Graduate Division of Biological and Biomedical Sciences, Emory University, Atlanta, GA 30322, USA.
authors.forename: E Shannon
authors.initials: ES
authors.lastname: Mills
authors.affiliation: Department of Biology, Emory University, Atlanta, GA 30322, USA.
authors.forename: Roger B
authors.initials: RB
authors.lastname: Deal
mesh_terms: D020869:Gene Expression Profiling; D023281:Genomics; D055442:Metabolome; D055432:Metabolomics; D009928:Organ Specificity; D059828:Plant Cells / Q000145:classification / Q000502:physiology*; D010944:Plants / Q000235:genetics* / Q000378:metabolism*; D020543:Proteome; D049490:Systems Biology / Q000379:methods*; D059467:Transcriptome
chemical_list: D020543:Proteome