title: High titer ethanol production from SPORL-pretreated lodgepole pine by simultaneous enzymatic saccharification and combined fermentation.
abstract: Lodgepole wood chips were pretreated by sulfite pretreatment to overcome recalcitrance of lignocelluloses (SPORL) at 25% solids loading and 180 °C for 20 min with sulfuric acid and sodium bisulfite charges of 2.2 and 8 wt/wt% on an oven-dry wood basis, respectively. The pretreated wood chips were disk-milled with pretreatment spent liquor and water, and the solid fraction was separated from the liquor stream. The liquor was neutralized and concentrated through vacuum evaporation. Quasi-simultaneous enzymatic saccharification of the cellulosic solids and combined fermentation with the concentrated liquor was conducted at up to 20% total solids loading. Fed-batching of the solids facilitated liquefaction and saccharification, as well as managing instantaneous inhibitor concentrations. At a commercial cellulase (CTec2) loading of only 9 FPU or 0.06 mL/g untreated wood, a maximum ethanol titer of 47.4 g/L was achieved, resulting in a calculated yield of 285 L/tonne of wood using Saccharomyces cerevisiae YRH400 at 35 °C and pH 5.5.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China.
authors.forename: T Q
authors.initials: TQ
authors.lastname: Lan
authors.forename: Roland
authors.initials: R
authors.lastname: Gleisner
authors.forename: J Y
authors.initials: JY
authors.lastname: Zhu
authors.forename: Bruce S
authors.initials: BS
authors.lastname: Dien
authors.forename: Ronald E
authors.initials: RE
authors.lastname: Hector
mesh_terms: D056804:Biofuels; D002480:Cellulase / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D028223:Pinus / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013447:Sulfites / Q000378:metabolism*; D013464:Sulfuric Acids
chemical_list: D056804:Biofuels; D013447:Sulfites; D013464:Sulfuric Acids; D000431:Ethanol; D002480:Cellulase; C033158:sulfuric acid; C009279:sodium bisulfite