title: Physical properties and in vitro evaluation of collagen-chitosan-calcium phosphate microparticle-based scaffolds for bone tissue regeneration.
abstract: Due to limitations of bone autografts and allografts, synthetic bone grafts using osteoconductive biomaterials have been designed. In this study, collagen-chitosan-calcium phosphate microparticle-based scaffolds fused with glycolic acid were compared to their counterparts without collagen in terms of degradation, cytocompatibility, porosity, and Young's modulus. It was found that 26-30% collagen was incorporated and that hydroxyapatite was present. Moreover, there were no differences between control and collagen scaffolds in degradation, cytocompatibility, porosity, and Young's modulus. In general, scaffolds exhibited 23% porosity, 0.6-1.2 MPa Young's modulus, 23% degradation over 4 weeks, and supported a four to seven fold increase in osteoblast cell number over 7 days in culture. Collagen can be incorporated into these bone graft substitute scaffolds, which show an improved degradation profile.
authors.affiliation: 1Department of Biomedical Engineering, University of Memphis, USA.
authors.forename: Monica V
authors.initials: MV
authors.lastname: Zugravu
authors.forename: Richard A
authors.initials: RA
authors.lastname: Smith
authors.forename: Benjamin T
authors.initials: BT
authors.lastname: Reves
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Jennings
authors.forename: Jared O
authors.initials: JO
authors.lastname: Cooper
authors.forename: Warren O
authors.initials: WO
authors.lastname: Haggard
authors.forename: Joel D
authors.initials: JD
authors.lastname: Bumgardner
mesh_terms: D001846:Bone Development; D002130:Calcium Phosphates / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D066298:In Vitro Techniques; D012038:Regeneration
chemical_list: D002130:Calcium Phosphates; D003094:Collagen; D048271:Chitosan; C020243:calcium phosphate
keywords: Chitosan; Young’s modulus; calcium phosphate; degradation; synthetic bone graft