title: Efficacy of subantimicrobial-dose doxycycline against nitrosative stress in chronic periodontitis.
abstract: OBJECTIVE
  To evaluate the effectiveness of subantimicrobial-dose doxycycline (SDD) as an adjunct to scaling and root planing (SRP) treatment against the nitrosative stress of moderate to advanced chronic periodontitis.
  
  
  METHODS
  Adults with untreated chronic periodontitis (n=174) were randomly administered SRP+SDD (n=87) (20 mg of doxycycline twice daily) or SRP+placebo (n=87) treatment for 3 months. At baseline and after 3 months, the probing depths (PD), bleeding on probing (BOP) and clinical attachment level (CAL) were measured, and a gingivomucosal biopsy was collected to assay the induction of nitric oxide synthase (iNOS) and 3-nitrotyrosine (3NT), and blood was collected to assay for total nitrites and nitrates (NO(x)) and 3NT.
  
  
  RESULTS
  Compared to baseline, at the completion of treatment, significant decreases in the levels of tissue iNOS and 3NT and serum NO(x) and 3NT were observed in both groups. SRP+SDD yielded a greater reduction in the gingivomucosal and serum nitrosative stress markers than did SRP+placebo. PD, BOP, and CAL reduction were correlated with the nitrosative stress parameters.
  
  
  CONCLUSIONS
  On a short-term basis, SDD therapy may be used as an adjunct to SRP treatment against nitrosative stress in moderate to advanced chronic periodontitis.
authors.affiliation: Department of Pathophysiology, Faculty of Medicine, University of Medicine and Pharmacy "Iuliu Haţieganu," Cluj-Napoca, Romania. parvualinaelena@yahoo.com
authors.forename: Alina Elena
authors.initials: AE
authors.lastname: Pârvu
authors.forename: Sandu Florin
authors.initials: SF
authors.lastname: Alb
authors.forename: Alexandra
authors.initials: A
authors.lastname: Crăciun
authors.forename: Marian Aurel
authors.initials: MA
authors.lastname: Taulescu
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D055113:Chronic Periodontitis / Q000097:blood* / Q000188:drug therapy* / Q000473:pathology; D012534:Dental Scaling / Q000379:methods; D004318:Doxycycline / Q000008:administration & dosage / Q000627:therapeutic use*; D005260:Female; D005881:Gingiva / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009566:Nitrates / Q000097:blood; D009569:Nitric Oxide / Q000097:blood*; D019001:Nitric Oxide Synthase / Q000032:analysis; D009573:Nitrites / Q000097:blood; D016745:Root Planing / Q000379:methods; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; D009566:Nitrates; D009573:Nitrites; D009569:Nitric Oxide; C002744:3-nitrotyrosine; D014443:Tyrosine; D019001:Nitric Oxide Synthase; D004318:Doxycycline