title: Crystallization and diffraction of an Isl1-Ldb1 complex.
abstract: A stable intramolecular complex comprising the LIM domains of the LIM-homeodomain protein Isl1 tethered to a peptide region of Ldb1 has been engineered, purified and crystallized. The orthorhombic crystals belonged to space group P222(1), with unit-cell parameters a=57.2, b=56.7, c=179.8 Å, and diffracted to 3.10 Å resolution.
authors.affiliation: School of Molecular Bioscience, University of Sydney, Sydney, NSW 2006, Australia. mgad4240@uni.sydney.edu.au
authors.forename: Morgan S
authors.initials: MS
authors.lastname: Gadd
authors.forename: David A
authors.initials: DA
authors.lastname: Jacques
authors.forename: J Mitchell
authors.initials: JM
authors.lastname: Guss
authors.forename: Jacqueline M
authors.initials: JM
authors.lastname: Matthews
mesh_terms: D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins / Q000737:chemistry*; D060588:LIM Domain Proteins / Q000737:chemistry*; D060850:LIM-Homeodomain Proteins / Q000737:chemistry*; D051379:Mice; D046912:Multiprotein Complexes / Q000737:chemistry; D017434:Protein Structure, Tertiary; D014157:Transcription Factors / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry
chemical_list: D004268:DNA-Binding Proteins; D060588:LIM Domain Proteins; D060850:LIM-Homeodomain Proteins; C102084:Ldb1 protein, mouse; D046912:Multiprotein Complexes; D014157:Transcription Factors; C063843:insulin gene enhancer binding protein Isl-1; D015032:Zinc