title: Overexpression of miR 156 in cotton via Agrobacterium-mediated transformation.
abstract: microRNAs (miRNAs) are an extensive class of newly identified endogenous small regulatory molecules. Many studies show that miRNAs play a critical role in almost all biological and metabolic progresses through targeting protein-coding genes for mRNA cleavage or translation inhibition. Many miRNAs are also identified from cotton using computational and/or experimental approaches, including the next generation deep sequencing technology. However, the function of the majority of miRNAs are unclear. In this chapter, we describe a detailed method for overexpressing miR 156 in cotton using Agrobacterium-mediated genetic transformation. This provides an approach to investigate the function and regulatory mechanism of miRNAs in cotton.
authors.affiliation: Department of Biology, East Carolina University, Greenville, NC, USA. zhangb@ecu.edu
authors.forename: Baohong
authors.initials: B
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Chengqi
authors.initials: C
authors.lastname: Li
authors.forename: Qinglian
authors.initials: Q
authors.lastname: Wang
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics*; D015870:Gene Expression; D017343:Genes, Plant / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D003368:Gossypium / Q000235:genetics* / Q000254:growth & development; D035683:MicroRNAs / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D035683:MicroRNAs; C559759:Mirn156 microRNA, Arabidopsis