title: The role of acetoacetyl-CoA synthetase, a ketone body-utilizing enzyme, in 3T3-L1 adipocyte differentiation.
abstract: Acetoacetyl-CoA synthetase (AACS) is a ketone body-utilizing enzyme that converts acetoacetate to acetoacetyl-CoA in the cytosol and consequently provides acetyl units as the precursors for lipogenesis. To clarify the role of AACS in adipogenesis, we investigated the expression and localization of the AACS protein and the effect of AACS knockdown on 3T3-L1 differentiation. The protein expression of AACS is dramatically induced during 3T3-L1 differentiation and is localized in the cytoplasm of differentiated 3T3-L1 cells. Moreover, knockdown of AACS inhibits differentiation of 3T3-L1 cells and suppresses expression of the adipocyte markers, peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer binding protein α (C/EBPα). These results suggest that AACS has a crucial role in the mechanism of 3T3-L1 differentiation.
authors.affiliation: Department of Health Chemistry, Hoshi University, Shinagawa-ku, Tokyo 142–8501, Japan. s-hasegawa@hoshi.ac.jp
authors.forename: Shinya
authors.initials: S
authors.lastname: Hasegawa
authors.forename: Yotaro
authors.initials: Y
authors.lastname: Ikeda
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yamasaki
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Fukui
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology*; D050156:Adipogenesis / Q000502:physiology*; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics; D003066:Coenzyme A Ligases / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D055785:Gene Knockdown Techniques; D007657:Ketone Bodies; D050155:Lipogenesis / Q000502:physiology; D051379:Mice; D047495:PPAR gamma / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D007657:Ketone Bodies; D047495:PPAR gamma; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D003066:Coenzyme A Ligases; C023747:acetoacetyl-CoA synthetase