title: Induction of immunological memory response by vaccination with TM4SF5             epitope-CpG-DNA-liposome complex in a mouse hepatocellular carcinoma model.
abstract: The innovation of a peptide vaccine strategy may contribute to the development of efficacious and convenient cancer vaccines. Recently, we formulated an efficacious peptide vaccine without carriers using the natural phosphodiester bond CpG-DNA and a special liposome complex [Lipoplex(O)]. The peptide vaccine targeting a tumor antigen, transmembrane 4 superfamily member 5 protein (TM4SF5), was confirmed to have preventive and therapeutic effects in a mouse hepatocellular carcinoma (HCC) model. In this study, we demonstrated that the isotype-switched (IgM(-)IgD(-)) B cell population increased after immunization and that the functional memory response persisted for at least 70 days after the final immunization of mice. Delayed implantation of BNL-HCC cells significantly induced the peptide-specific IgG2a production in the immunized mice. Accordingly, tumor growth was inhibited and the survival rate increased. These results suggest that our peptide vaccine induces memory response, which is essential for cancer vaccine application.
authors.affiliation: Center for Medical Science Research, College of Medicine, Hallym                     University, Gangwon, Republic of Korea.
authors.forename: Sanghoon
authors.initials: S
authors.lastname: Kwon
authors.forename: Dongbum
authors.initials: D
authors.lastname: Kim
authors.forename: Byoung Kwon
authors.initials: BK
authors.lastname: Park
authors.forename: Guang
authors.initials: G
authors.lastname: Wu
authors.forename: Min Chul
authors.initials: MC
authors.lastname: Park
authors.forename: Yang-Wha
authors.initials: YW
authors.lastname: Ha
authors.forename: Hyung-Joo
authors.initials: HJ
authors.lastname: Kwon
authors.forename: Younghee
authors.initials: Y
authors.lastname: Lee
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D019496:Cancer Vaccines / Q000276:immunology* / Q000627:therapeutic use; D006528:Carcinoma, Hepatocellular / Q000276:immunology* / Q000517:prevention & control; D045744:Cell Line, Tumor; D018899:CpG Islands / Q000276:immunology; D004195:Disease Models, Animal; D000939:Epitopes / Q000276:immunology; D017578:Immunoglobulin Class Switching; D007074:Immunoglobulin G / Q000276:immunology; D007156:Immunologic Memory / Q000276:immunology*; D053208:Kaplan-Meier Estimate; D008081:Liposomes; D008113:Liver Neoplasms / Q000276:immunology* / Q000517:prevention & control; D008297:Male; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice
chemical_list: D019496:Cancer Vaccines; D000939:Epitopes; D007074:Immunoglobulin G; D008081:Liposomes; D008565:Membrane Proteins; C570858:TM4SF5 protein, mouse