title: Neuropeptides isotocin and arginine vasotocin in urophysis of three fish species.
abstract: In this study, for the first time, both neuropeptides isotocin (IT) and arginine vasotocin (AVT) have been identified and measured in urophysis, the neurohaemal organ of the caudal neurosecretory system of teleost fish. So far, AVT, but not IT, was quantified by radioimmunoassay (RIA) in urophysis of several fish species. We have used high-performance liquid chromatographic assay with fluorescence detection (HPLC-FL) preceded by solid-phase extraction (SPE) and liquid chromatography-electrospray ionization triple-quadrupole tandem mass spectrometry (LC-ESI MS/MS) technique to determine both neuropeptides in urophysis of three fish species. The efficiency of peptide's SPE extraction was 79-85%. In HPLC-FL method, the limits of detection (LOD) and quantification (LOQ) were estimated as 1.0 and 3.4 pmol/mL for IT and 0.25 and 2.20 pmol/mL for AVT. In LC-MS/MS method, LOD and LOQ were estimated as 0.4 and 1.2 pmol/mL for IT and 0.06 and 0.2 pmol/mL for AVT. The chromatographic methods are good alternative for RIA, because enable to measure both nonapeptides simultaneously in one sample. In round goby (Neogobius melanostomus), three-spined stickleback (Gasterosteus aculeatus) and sea bream (Sparus aurata), urophysial IT concentrations ranged between 0.056 and 0.678 pmol/mg tissue and AVT concentrations ranged between 0.0008 (or even below detection threshold) and 0.084 pmol/mg tissue.
authors.affiliation: Department of Genetics and Marine Biotechnology, Institute of Oceanology of Polish Academy of Sciences, Powstańców Warszawy 55 St., 81-712 Sopot, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Gozdowska
authors.forename: Marek
authors.initials: M
authors.lastname: Ślebioda
authors.forename: Ewa
authors.initials: E
authors.lastname: Kulczykowska
mesh_terms: D000818:Animals; D009490:Neurosecretory Systems / Q000378:metabolism*; D010121:Oxytocin / Q000031:analogs & derivatives* / Q000378:metabolism; D010473:Perciformes / Q000378:metabolism*; D023701:Smegmamorpha / Q000378:metabolism*; D014668:Vasotocin / Q000378:metabolism*
chemical_list: D010121:Oxytocin; C008215:isotocin; D014668:Vasotocin