title: Association of the gene encoding stearoyl-CoA desaturase (SCD) with fatty acid composition in an intercross population between Landrace and Korean native pigs.
abstract: Fatty acid (FA) composition is one of the most important parameters for the evaluation of meat quality. The stearoyl-CoA desaturase (SCD) gene is considered a positional candidate gene affecting FA composition in SSC14, based on previous quantitative trait loci studies. To evaluate the association of the SCD gene with FA composition in a Korean native pig × Landrace F(2) intercross population, we genotyped six single nucleotide polymorphisms (SNPs) of the SCD gene located in promoter region [2 SNPs (g.-353T>C, g.-233T>C)], exonic region [1 SNP (g.817C>T) in exon 2] and 3' UTR [3 SNPs (g.13311C>G, g.14384G>A, and g.14424C>T)] identified by massively parallel sequencing technology. Eighteen FA composition traits were measured in more than the 950 F(2) animals. A mixed-effect model was used to evaluate associations between these SNPs and FA composition traits in the F(2) intercross population. A detailed investigation detected that the five FA composition traits [palmitoleic acid (C16:1), stearic acid (C18:0), arachidic acid (C20:0), saturated FA, and unsaturated FA] were highly significant (P < 4.7 × 10(-5); C20:0) in association with the SNP g.-233T>C, SNP g.817C>T, SNP g.13311C>G and SNP g.14384G>A in the SCD gene, whereas SNP g.14424C>T was only significantly associated with palmitoleic acid (C16:1, P = 1.4 × 10(-3)). No significant association of FA composition traits with SNP g.-353T>C was detected. In particular, the SNP g.14384G>A accounted for 30.6 % of the additive genetic variance of palmitoleic acid (P = 1.9 × 10(-10)). These results suggest the SCD gene has a strong effect on FA composition in the crossbred pig population.
authors.affiliation: Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Dyah
authors.initials: D
authors.lastname: Maharani
authors.forename: Hee-Bok
authors.initials: HB
authors.lastname: Park
authors.forename: Jae-Bong
authors.initials: JB
authors.lastname: Lee
authors.forename: Chae-Kyoung
authors.initials: CK
authors.lastname: Yoo
authors.forename: Hyun-Tae
authors.initials: HT
authors.lastname: Lim
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Han
authors.forename: Sung-Soo
authors.initials: SS
authors.lastname: Lee
authors.forename: Moon-Suck
authors.initials: MS
authors.lastname: Ko
authors.forename: In-Cheol
authors.initials: IC
authors.lastname: Cho
authors.forename: Jun-Heon
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000483:Alleles; D000818:Animals; D003433:Crosses, Genetic; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D005787:Gene Frequency; D023061:Gene Order; D005838:Genotype; D008297:Male; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D013230:Stearoyl-CoA Desaturase / Q000235:genetics*; D013552:Swine / Q000235:genetics*
chemical_list: D005227:Fatty Acids; D013230:Stearoyl-CoA Desaturase