title: Adhesion and biologic behavior of human periodontal fibroblast cells to resin ionomer Geristore: a comparative analysis.
abstract: OBJECTIVE
  The resin ionomer Geristore, originally designed for restorative procedures, has been used extensively in treating subgingival defects (such as root resorption and perforations) and as a retrofilling material. The purpose of this study was to evaluate the cell adhesion as well as in vitro biocompatibility of human periodontal fibroblast cells with resin ionomer Geristore in comparison with mineral trioxide aggregate (MTA) and glass ionomer cement (GIC).
  
  
  METHODS
  Adhesion, growth, and morphology of human periodontal fibroblasts over test materials were evaluated by scanning electron microscopy (SEM). Biocompatibility was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide salt (MTT) assay.
  
  
  RESULTS
  Compared to glass coverslips, cells grew and spread qualitatively better over the surface of Geristore in comparison with the other test materials. In vitro interpretation indicates that Geristore is significantly less cytotoxic to human periodontal ligament cells. Results of statistical analysis revealed that material extracts had significant effect on cell proliferation at both 24 h (F = 547.62, P < 0.05) and at 48 h (F = 6048.18, P < 0.05).
  
  
  CONCLUSIONS
  Our study supports that Geristore has enhanced biologic behavior to human periodontal ligament cells and superior biocompatibility in comparison with MTA and GIC, so it can be suggested as a material of choice in root resorption, perforations, and root-end filling.
authors.affiliation: Department of Conservative Dentistry & Endodontics, Government College of Dentistry, Indore, India.
authors.forename: Saurabh Kumar
authors.initials: SK
authors.lastname: Gupta
authors.forename: Payal
authors.initials: P
authors.lastname: Saxena
authors.forename: Vandana Aditya
authors.initials: VA
authors.lastname: Pant
authors.forename: Aditya Bhushan
authors.initials: AB
authors.lastname: Pant
mesh_terms: D002448:Cell Adhesion; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000648:ultrastructure; D005899:Glass Ionomer Cements; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010519:Periodontium / Q000166:cytology* / Q000648:ultrastructure; D012117:Resins, Synthetic
chemical_list: C074933:Geristore; D005899:Glass Ionomer Cements; D012117:Resins, Synthetic
keywords: biocompatibility; cell adhesion; cytotoxicity assay; periodontal ligament cells; subgingival defects