title: Distribution of Zonula Occludens-1 and Occludin and alterations of testicular morphology after in utero radiation and postnatal hyperthermia in rats.
abstract: In utero irradiation (IR) and postnatal hyperthermia (HT) exposure cause infertility by decreasing spermatogenic colony growth and the number of sperm in rats. Four groups were used: (i) Control group, (ii) HT group (rats exposed to hyperthermia on the 10th postnatal day), (iii) IR group (rats exposed to IR on the 17th gestational day) and (iv) IR + HT group. Three and six months after the procedures testes were examined by light and electron microscopy. Some degenerated tubules in the HT group, many vacuoles in spermatogenic cells and degenerated tight junctions in the IR group, atrophic tubules and severe degeneration of tight junctions in the IR + HT group were observed. ZO-1 and occludin immunoreactivity were decreased and disorganized in the HT and IR groups and absent in the IR + HT group. The increase in the number of apoptotic cells was accompanied by a time-dependent decrease in haploid, diploid and tetraploid cells in all groups. Degenerative findings were severe after 6 months in all groups. The double-hit model may represent a Sertoli cell only model of infertility due to a decrease in spermatogenic cell and alterated blood-testis barrier proteins in rat.
authors.affiliation: Department of Histology and Embryology, School of Medicine, Marmara University, Istanbul, Turkey. gozdeerkanli@yahoo.com
authors.forename: Gozde
authors.initials: G
authors.lastname: Erkanlı Şentürk
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Ersoy Canillioĝlu
authors.forename: Cenk
authors.initials: C
authors.lastname: Umay
authors.forename: Emel
authors.initials: E
authors.lastname: Demiralp-Eksioglu
authors.forename: Feriha
authors.initials: F
authors.lastname: Ercan
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D005260:Female; D005298:Fertility / Q000502:physiology / Q000528:radiation effects; D005434:Flow Cytometry; D006979:Hyperthermia, Induced / Q000009:adverse effects*; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects*; D062793:Occludin / Q000378:metabolism*; D009929:Organ Size / Q000528:radiation effects; D011003:Ploidies; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000209:etiology* / Q000473:pathology; D011829:Radiation Dosage; D011832:Radiation Injuries / Q000150:complications* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012708:Sertoli Cells / Q000473:pathology / Q000528:radiation effects; D013737:Testis / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D019108:Tight Junctions / Q000528:radiation effects / Q000648:ultrastructure; D013997:Time Factors; D062826:Zonula Occludens-1 Protein / Q000378:metabolism*
chemical_list: D062793:Occludin; C569044:Tjp1 protein, rat; D062826:Zonula Occludens-1 Protein