title: Non-neutral evolution in non-LEE-encoded type III effectors of attaching and effacing Escherichia coli.
abstract: Attaching and effacing Escherichia coli (AEEC) employ type III secretion system (T3SS) to secrete effector proteins into host cells and regulate their function. Here we have investigated T3SS genes of AEEC for non-neutral evolution. Our analysis revealed non-neutral evolution in three genes (nleE1, nleB2 and nleD) which encode effector proteins. These genes are located outside the locus of enterocyte effacement (LEE). In general, non-LEE effector genes show greater deviation from neutral evolution than LEE effector genes. These results suggest that effector genes located outside LEE are under greater selection pressure than those present in LEE.
authors.affiliation: Department of Cellular and Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA. eswaras@ccf.org
authors.forename: Sandeepa M
authors.initials: SM
authors.lastname: Eswarappa
authors.forename: Jessin
authors.initials: J
authors.lastname: Janice
authors.forename: Sudhagar V
authors.initials: SV
authors.lastname: Balasundaram
authors.forename: Dipshikha
authors.initials: D
authors.lastname: Chakravortty
mesh_terms: D001422:Bacterial Adhesion / Q000235:genetics*; D058947:Bacterial Secretion Systems / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D019143:Evolution, Molecular; D005798:Genes, Bacterial; D040961:Genetic Drift; D014644:Genetic Variation; D010750:Phosphoproteins / Q000235:genetics*; D012641:Selection, Genetic; D037521:Virulence Factors / Q000235:genetics
chemical_list: D058947:Bacterial Secretion Systems; D029968:Escherichia coli Proteins; C457340:LEE protein, E coli; C553814:NleB protein, E coli; C526043:NleE protein, E coli; D010750:Phosphoproteins; D037521:Virulence Factors; C506689:Z0990 protein, E coli