title: New triterpenes from Maytenus robusta: structural elucidation based on NMR experimental data and theoretical calculations.
abstract: Leaves of Maytenus robusta (Celastraceae) were subjected to phytochemical investigation mainly directed at the isolation of pentacyclic triterpenes. The compounds friedelin (1), &#946;-friedelinol (2), 3-oxo-21&#946;-H-hop-22(29)-ene (7), 3,4-seco-friedelan-3,11&#946;-olide (8), 3&#946;-hydroxy-21&#946;-H-hop-22(29)-ene (9), 3,4-seco-21&#946;-H-hop-22(29)-en-3-oic acid (10), 3,4-seco-friedelan-3-oic acid (11), and sitosterol were identified in the hexane extract of M. robusta leaves. Compounds 8 and 9 are described herein for the first time. The structure and stereochemistry of both compounds were experimentally established by IR, HRLC-MS, and 1D (1H, 13C, and DEPT 135) and 2D (HSQC, HMBC and COSY) NMR data and supported by correlations with carbon chemical shifts calculated using the DFT method (BLYP/6-31G* level). Compounds 7 and 10 are also described for the first time, and their chemical structures were established by comparison with NMR data of similar structures described in the literature and correlations with BLYP/6-31G* calculated carbon chemical shifts. Compound 9, a mixture of 11 and sitosterol, and 3&#946;,11&#946;-dihydroxyfriedelane (4) were evaluated by the Ellman&#8217;s method and all these compounds showed acethylcholinesterase inhibitory properties.
authors.affiliation: Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, 31270-901, Belo Horizonte, MG, Brazil.
authors.forename: Grasiely F
authors.initials: GF
authors.lastname: Sousa
authors.forename: Lucienir P
authors.initials: LP
authors.lastname: Duarte
authors.forename: Antônio F C
authors.initials: AF
authors.lastname: Alcântara
authors.forename: Grácia D F
authors.initials: GD
authors.lastname: Silva
authors.forename: Sidney A
authors.initials: SA
authors.lastname: Vieira-Filho
authors.forename: Roqueline R
authors.initials: RR
authors.lastname: Silva
authors.forename: Djalma M
authors.initials: DM
authors.lastname: Oliveira
authors.forename: Jacqueline A
authors.initials: JA
authors.lastname: Takahashi
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry; D002725:Chloroform / Q000737:chemistry; D002800:Cholinesterase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D006586:Hexanes / Q000737:chemistry; D059625:Liquid-Liquid Extraction; D009682:Magnetic Resonance Spectroscopy; D029755:Maytenus / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D002800:Cholinesterase Inhibitors; D006586:Hexanes; D010936:Plant Extracts; D012997:Solvents; D014315:Triterpenes; D002725:Chloroform; D000110:Acetylcholinesterase