title: Using nanoscale substrate curvature to control the dimerization of a surface-bound protein.
abstract: The influence of surface geometry on adsorbed proteins offers new possibilities for controlling quaternary structure by manipulating protein-protein interactions at a surface, with applications that are relevant to protein aggregation, fibrillation, ligand binding, and surface catalysis. To understand the effect of surface curvature on the structure of the surface-bound protein β-lactoglobulin (β-LG), we have used a combination of polystyrene (PS) nanoparticles (NPs) and ultrathin PS films to fabricate chemically pure, hydrophobic surfaces that have nanoscale curvature and are stable in aqueous buffer. We have used single molecule force spectroscopy to measure the detachment contour lengths L(c) for β-LG adsorbed on the highly curved PS surfaces, and we compare these values in situ to those measured for β-LG adsorbed on flat PS surfaces on the same samples. The L(c) distributions measured on all flat PS surfaces show a large monomer peak near 60 nm and a smaller dimer peak at 120 nm. For 190 and 100 nm diameter NPs, which are effectively flat on the scale of the β-LG molecules, there is no measurable difference between the L(c) distributions obtained for the flat and curved surfaces. However, for 60 nm diameter NPs the dimer peak is smaller, and for 25 nm diameter NPs the dimer peak is absent, indicating that the number of surface-bound dimers is significantly reduced by an increase in the curvature of the underlying surface. These results indicate that surface curvature provides a new method of manipulating protein-protein interactions and controlling the quaternary structure of adsorbed proteins.
authors.affiliation: Department of Physics, University of Guelph, Guelph ON, Canada N1G 2W1.
authors.forename: Martin
authors.initials: M
authors.lastname: Kurylowicz
authors.forename: Maximiliano
authors.initials: M
authors.lastname: Giuliani
authors.forename: John R
authors.initials: JR
authors.lastname: Dutcher
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D057927:Hydrophobic and Hydrophilic Interactions; D007782:Lactoglobulins / Q000737:chemistry* / Q000378:metabolism; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D013499:Surface Properties
chemical_list: D007782:Lactoglobulins; D011137:Polystyrenes