title: Crystallization and first data collection of the putative transfer protein TraN from the Gram-positive conjugative plasmid pIP501.
abstract: Conjugative plasmid transfer is the most important route for the spread of resistance and virulence genes among bacteria. Consequently, bacteria carrying conjugative plasmids are a substantial threat to human health, especially hospitalized patients. Whilst detailed information about the process has been obtained for Gram-negative type-4 secretion systems, little is known about the corresponding mechanisms in Gram-positive (G+) bacteria. The successful purification and crystallization of the putative transfer protein TraN from the G+ conjugative model plasmid pIP501 of Enterococcus faecalis are presented. Native crystals diffracted to 1.8 Å resolution on a synchrotron beamline. The crystals belonged to space group P2(1), with unit-cell parameters a=32.88, b=54.94, c=57.71 Å, β=91.89° and two molecules per asymmetric unit.
authors.affiliation: Institute for Molecular Biosciences, Karl-Franzens-University Graz, Humboldtstrasse 50/III, 8010 Graz, Austria. nikolaus.goessweiner-mohr@uni-graz.at
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Goessweiner-Mohr
authors.forename: Christian
authors.initials: C
authors.lastname: Fercher
authors.forename: Mohammad Yaser
authors.initials: MY
authors.lastname: Abajy
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Grohmann
authors.forename: Walter
authors.initials: W
authors.lastname: Keller
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003227:Conjugation, Genetic; D003460:Crystallization; D018360:Crystallography, X-Ray; D013293:Enterococcus faecalis / Q000235:genetics*; D004926:Escherichia coli; D010957:Plasmids / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins