title: Studying the roles of Aurora-C kinase during meiosis in mouse oocytes.
abstract: We previously isolated Aurora-C (Aurkc/Aie1) in a screen for kinases expressed in mouse sperm and eggs. Aurora-C kinase was reported to be a chromosomal passenger protein that plays critical roles in chromosome alignment, segregation, kinetochore-microtubule attachment, and cytokinesis in female mouse meiosis. This chapter describes experimental approaches for examining the subcellular localization and function of Aurora-C kinase during female mouse meiosis, presenting detailed methods for introducing exogenous Aurora-C wild-type and kinase-dead mutant mRNAs into mouse oocytes by cytosolic microinjection, and preparing whole-mount meiotic oocytes and chromosome spreads for confocal immunofluorescence microscopy.
authors.affiliation: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
authors.forename: Kuo-Tai
authors.initials: KT
authors.lastname: Yang
authors.forename: Yi-Nan
authors.initials: YN
authors.lastname: Lin
authors.forename: Shu-Kuei
authors.initials: SK
authors.lastname: Li
authors.forename: Tang K
authors.initials: TK
authors.lastname: Tang
mesh_terms: D000818:Animals; D064108:Aurora Kinase C; D064127:Aurora Kinases; D002469:Cell Separation; D033481:Chromosomes, Mammalian / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D057075:Enzyme Assays / Q000379:methods*; D005260:Female; D005455:Fluorescent Antibody Technique; D008540:Meiosis; D051379:Mice; D008810:Mice, Inbred C57BL; D008845:Microinjections; D009865:Oocytes / Q000166:cytology* / Q000201:enzymology* / Q000378:metabolism; D010053:Ovary / Q000166:cytology; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; C576928:Aurkc protein, mouse; D064108:Aurora Kinase C; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases