title: Single-round, multiplexed antibody mimetic design through mRNA display.
abstract: In a single round: By combining the high-efficiency enrichment through the continuous-flow magnetic separation (CFMS) technique with the analytical power of next-generation sequencing, the generation of antibody mimetics with a single round of mRNA display is made possible. This approach eliminates iterative selection cycles and provides a path to fully automated ligand generation (see picture).
authors.affiliation: Department of Molecular and Medical Pharmacology, University of California, Los Angeles, CA 90095, USA.
authors.forename: C Anders
authors.initials: CA
authors.lastname: Olson
authors.forename: Jeff
authors.initials: J
authors.lastname: Nie
authors.forename: Jonathan
authors.initials: J
authors.lastname: Diep
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Al-Shyoukh
authors.forename: Terry T
authors.initials: TT
authors.lastname: Takahashi
authors.forename: Laith Q
authors.initials: LQ
authors.lastname: Al-Mawsawi
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Bolin
authors.forename: Angela L
authors.initials: AL
authors.lastname: Elwell
authors.forename: Scott
authors.initials: S
authors.lastname: Swanson
authors.forename: Ron
authors.initials: R
authors.lastname: Stewart
authors.forename: James A
authors.initials: JA
authors.lastname: Thomson
authors.forename: H Tom
authors.initials: HT
authors.lastname: Soh
authors.forename: Richard W
authors.initials: RW
authors.lastname: Roberts
authors.forename: Ren
authors.initials: R
authors.lastname: Sun
mesh_terms: D000595:Amino Acid Sequence; D000906:Antibodies / Q000737:chemistry / Q000378:metabolism; D040761:Biomimetic Materials / Q000737:chemistry / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005353:Fibronectins / Q000737:chemistry / Q000378:metabolism; D015723:Gene Library; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry / Q000378:metabolism; D018189:Immunomagnetic Separation; D008024:Ligands; D058619:Maltose-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D000906:Antibodies; D005353:Fibronectins; D007141:Immunoglobulin Fc Fragments; D008024:Ligands; D058619:Maltose-Binding Proteins; D012333:RNA, Messenger