title: eNOS genotype modifies the effect of leisure-time physical activity on serum triglyceride levels in a Japanese population.
abstract: BACKGROUND
  Nitric oxide is a key molecule not only in the cardiovascular system, but also in the metabolic-endocrine system. The purpose of this study was to examine possible associations of the NOS3 T-786C polymorphism (rs2070744) with serum lipid levels on the basis of lifestyle factors for tailoring prevention of dyslipidemia.
  
  
  METHODS
  For this cross-sectional study, a total of 2226 subjects aged 35 to 69 years (1084 men and 1142 women) were selected from Japanese participants in the Japan Multi-Institutional Collaborative Cohort (J-MICC) Study. They were recruited in eight areas throughout Japan between February 2004 and November 2008.
  
  
  RESULTS
  In a stratified analysis by leisure-time physical activity, the likelihood of hypertriglyceridemia (serum triglyceride levels ≥ 150 mg/dL) among subjects with the C allele was significantly lower than those without it in the active group (OR = 0.43, 95% CI = 0.22-0.84 in the fasting group), but not in the sedentary group. A gene-environment interaction between the T-786C polymorphism and leisure-time physical activity for hypertriglyceridemia was significant (P = 0.007 in the fasting group). Additionally, serum triglyceride levels (mean ± SD) across leisure-time physical activity classes decreased significantly only in the TC + CC genotype group (111 ± 60 mg/dL for sedentary, 95 ± 48 mg/dL for moderately active, 88 ± 44 mg/dL for very active, P for trend = 0.008 in the fasting group), but not in the TT genotype group. Total cholesterol, high-density lipoprotein (HDL) cholesterol, and non-HDL cholesterol levels had no significant association with the polymorphism.
  
  
  CONCLUSIONS
  This study suggests that the NOS3 T-786C polymorphism modifies the effect of leisure-time physical activity on serum triglyceride levels.
authors.affiliation: Department of Preventive Medicine, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan. h-bata@med.nagoya-u.ac.jp
authors.forename: Takahiro
authors.initials: T
authors.lastname: Higashibata
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hamajima
authors.forename: Mariko
authors.initials: M
authors.lastname: Naito
authors.forename: Sayo
authors.initials: S
authors.lastname: Kawai
authors.forename: Guang
authors.initials: G
authors.lastname: Yin
authors.forename: Sadao
authors.initials: S
authors.lastname: Suzuki
authors.forename: Yoshikuni
authors.initials: Y
authors.lastname: Kita
authors.forename: Hideshi
authors.initials: H
authors.lastname: Niimura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Imaizumi
authors.forename: Keizo
authors.initials: K
authors.lastname: Ohnaka
authors.forename: Kokichi
authors.initials: K
authors.lastname: Arisawa
authors.forename: Masako
authors.initials: M
authors.lastname: Shigeta
authors.forename: Hidemi
authors.initials: H
authors.lastname: Ito
authors.forename: Haruo
authors.initials: H
authors.lastname: Mikami
authors.forename: Michiaki
authors.initials: M
authors.lastname: Kubo
authors.forename: Hideo
authors.initials: H
authors.lastname: Tanaka
authors.forename: Kenji
authors.initials: K
authors.lastname: Wakai
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D050171:Dyslipidemias / Q000235:genetics / Q000503:physiopathology; D005260:Female; D056726:Genetic Association Studies; D006801:Humans; D007564:Japan; D007899:Leisure Activities; D008297:Male; D008875:Middle Aged; D009043:Motor Activity; D052250:Nitric Oxide Synthase Type III / Q000097:blood / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D014280:Triglycerides / Q000097:blood / Q000235:genetics
chemical_list: D014280:Triglycerides; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III