title: Establishment of glass catfish (Kryptopterus bicirrhis) fin-derived cells.
abstract: Genetically manipulated transparent animals were already explored in many species for in vivo study of gene expression, transplantation analysis and cancer biology. However, there are no reports about transparent animals as in vitro genetic resources. In the present study, fin-derived cells from glass catfish (Krytopterus bicirrhis), naturally transparent fish with a visible skeleton and internal organs, were isolated after culturing fin explants and characterized using cryopreservation and cell cycle analysis. The cells grew well in DMEM (Dulbecco's modified Eagle's medium) containing 1% (v/v) P/S (penicillin-streptomycin) and 10% (v/v) fetal bovine serum at 26°C and showed increased cryopreservation efficiency with the slow-freezing method in the presence of 15% dimethyl sulfoxide. In addition, cell cycle analysis was evaluated based on flow cytometric analysis, and culturing to confluence (>85%) was more effective for synchronizing cells at the G(0)/G(1) stages than roscovitine treatment (<75%). This is the first report about cell isolation from transparent animals. The results from testing the cell's viability following cryopreservation and subjecting the cells to cycle analysis can be useful tools for genetic resource management.
authors.affiliation: Laboratory of Aquatic Animal Medicine.
authors.forename: Jee Eun
authors.initials: JE
authors.lastname: Han
authors.forename: Casiano H
authors.initials: CH
authors.lastname: Choresca
authors.forename: Ok Jae
authors.initials: OJ
authors.lastname: Koo
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Oh
authors.forename: So Gun
authors.initials: SG
authors.lastname: Hong
authors.forename: Ji Hyung
authors.initials: JH
authors.lastname: Kim
authors.forename: Sang Phil
authors.initials: SP
authors.lastname: Shin
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Jun
authors.forename: Byeong Chun
authors.initials: BC
authors.lastname: Lee
authors.forename: Se Chang
authors.initials: SC
authors.lastname: Park
keywords: DMEM, Dulbecco's modified Eagle's medium; FBS, fetal bovine serum; P/S, penicillin–streptomycin; SCNT, somatic cell nuclear transfer; cell cryopreservation; cell cycle analysis; cell isolation; fin cells; glass catfish