title: Sequential changes in the non-coding control region sequences of JC polyomaviruses from the cerebrospinal fluid of patients with progressive multifocal leukoencephalopathy.
abstract: Progressive multifocal leukoencephalopathy (PML) is caused by JC polyomavirus (JCV) infection in the brain. JCV isolates from PML patients have variable mutations in the non-coding control region (NCCR) of the genome. This study was conducted to examine sequential changes in NCCR patterns of JCV isolates obtained from the cerebrospinal fluid (CSF) of PML patients. CSF specimens were collected from PML patients at different time points, the NCCR sequences were determined, and their compositions were assessed by computer-based analysis. In patients showing a marked increase in JCV load, the most frequent NCCR sequences in the follow-up specimens were different from those in the initial samples. In contrast, the dominant NCCRs in the CSF remained unaltered during the follow-up of individuals in whom the viral load decreased after therapeutic intervention. These data demonstrate that the majority of JCV variants emerge with the progression of PML and that these changes are suppressed when the viral load is decreased.
authors.affiliation: Department of Virology 1, National Institute of Infectious Diseases, Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nakamichi
authors.forename: Shuji
authors.initials: S
authors.lastname: Kishida
authors.forename: Kozue
authors.initials: K
authors.lastname: Tanaka
authors.forename: Akihiko
authors.initials: A
authors.lastname: Suganuma
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Sano
authors.forename: Hironori
authors.initials: H
authors.lastname: Sano
authors.forename: Takashi
authors.initials: T
authors.lastname: Kanda
authors.forename: Norihisa
authors.initials: N
authors.lastname: Maeda
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Kira
authors.forename: Ai
authors.initials: A
authors.lastname: Itoh
authors.forename: Natsuko
authors.initials: N
authors.lastname: Kato
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Tomimoto
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kurane
authors.forename: Chang-Kweng
authors.initials: CK
authors.lastname: Lim
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Mizusawa
authors.forename: Masayuki
authors.initials: M
authors.lastname: Saijo
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D001921:Brain / Q000821:virology; D004279:DNA, Viral / Q000032:analysis / Q000134:cerebrospinal fluid / Q000235:genetics; D005260:Female; D016679:Genome, Viral; D006801:Humans; D007577:JC Virus / Q000235:genetics* / Q000302:isolation & purification*; D007968:Leukoencephalopathy, Progressive Multifocal / Q000134:cerebrospinal fluid / Q000821:virology*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D012045:Regulatory Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D019562:Viral Load
chemical_list: D004279:DNA, Viral