title: Cellular interactions with photo-cross-linked and pH-sensitive polymersomes: biocompatibility and uptake studies.
abstract: Polymeric nanoparticles, specifically polymersomes, are at the leading edge of the rapidly developing field of nanotechnology. However, their use for biological applications is primarily limited by the biocompatibility of the components. Hence, optimization of polymersome synthesis protocols should carefully consider aspects of cellular toxicity. In this work, we investigate the viability of HDF and HeLa cells treated with photo-cross-linked and pH-sensitive polymersomes. We demonstrate how aspects of polymersome preparation conditions such as cross-linking density and UV irradiation time may affect their cytotoxic properties. Additionally, we also study the cellular uptake of our polymersomes into the cell types mentioned.
authors.affiliation: Leibniz-Institut für Polymerforschung Dresden e.V., Hohe Straße 6, 01069 Dresden, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Gaitzsch
authors.forename: Irene
authors.initials: I
authors.lastname: Canton
authors.forename: Dietmar
authors.initials: D
authors.lastname: Appelhans
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Battaglia
authors.forename: Brigitte
authors.initials: B
authors.lastname: Voit
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002460:Cell Line; D003432:Cross-Linking Reagents; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D011108:Polymers