title: Cell-mediated immune response to epitopic MAP (multiple antigen peptide) construct of LcrV antigen of Yersinia pestis in murine model.
abstract: Yersinia pestis is the causative agent of plague. Cellular immunity seems to play an important role in defense against this disease. The subunit vaccine based on V (Lcr V) antigen has been proved to be immunogenic in animals and in humans. The multiple antigen peptide (MAP), incorporating all the relevant B and T cell epitopes is highly immunogenic in mice through intranasal route of immunization in PLGA particles containing CpG-ODN as an immunoadjuvant inducing humoral and mucosal immune response. In the present study, cell-mediated immune response using same MAP was studied in murine model. Primary and memory T cell responses were studied in outbred and inbred mice immunized intranasally with MAP in the presence of two immunoadjuvants (Murabutide and CpG-ODN). All the three compartments (Spleen, Lamina propria and Peyer's patches) of the lymphoid system showed increased lymphoproliferative response. Highest lymphoproliferative response was observed especially with CpG-ODN. Cytokine profile in the culture supernatant showed highest Th(1) and Th(17) levels. FACS analysis showed expansion of both CD4(+) and CD8(+) T cells producing gamma-interferon, perforin and granzyme-B with major contribution from CD4(+) T cells.
authors.affiliation: All India Institute of Medical Sciences, New Delhi 110029, India.
authors.forename: Rupesh Kumar
authors.initials: RK
authors.lastname: Shreewastav
authors.forename: Riyasat
authors.initials: R
authors.lastname: Ali
authors.forename: Jayaprakash Babu
authors.initials: JB
authors.lastname: Uppada
authors.forename: D N
authors.initials: DN
authors.lastname: Rao
mesh_terms: D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000008:administration & dosage / Q000031:analogs & derivatives; D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000281:Administration, Intranasal; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D015703:Antigens, CD / Q000096:biosynthesis / Q000276:immunology; D004195:Disease Models, Animal; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D053804:Granzymes / Q000096:biosynthesis / Q000276:immunology; D007111:Immunity, Cellular; D007156:Immunologic Memory; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage; D010455:Peptides / Q000008:administration & dosage* / Q000276:immunology; D010930:Plague / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D010931:Plague Vaccine / Q000276:immunology*; D052899:Pore Forming Cytotoxic Proteins / Q000096:biosynthesis / Q000276:immunology*; D018417:Th1 Cells / Q000166:cytology / Q000276:immunology; D058504:Th17 Cells / Q000166:cytology / Q000276:immunology; D022223:Vaccines, Subunit / Q000276:immunology; D015010:Yersinia pestis / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000942:Antigens, Bacterial; D015703:Antigens, CD; C408982:CPG-oligonucleotide; D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; C043908:LcrV protein, Yersinia; D009838:Oligodeoxyribonucleotides; D010455:Peptides; D010931:Plague Vaccine; D052899:Pore Forming Cytotoxic Proteins; D022223:Vaccines, Subunit; C483886:perforin, mouse; D000119:Acetylmuramyl-Alanyl-Isoglutamine; D007371:Interferon-gamma; C033575:murabutide; D053804:Granzymes; C509214:Gzmb protein, mouse