title: A standardised protocol for the validation of banking methodologies for arterial allografts.
abstract: The objective of this study was to design and test a protocol for the validation of banking methodologies for arterial allografts. A series of in vitro biomechanical and biological assessments were derived, and applied to paired fresh and banked femoral arteries. The ultimate tensile stress and strain, suture pullout stress and strain, expansion/rupture under hydrostatic pressure, histological structure and biocompatibility properties of disinfected and cryopreserved femoral arteries were compared to those of fresh controls. No significant differences were detected in any of the test criteria. This validation protocol provides an effective means of testing and validating banking protocols for arterial allografts.
authors.affiliation: National Health Service Blood and Transplant Tissue Services, Liverpool Centre, 14 Estuary Banks, Speke, Liverpool, L24 8RB, UK. richard.lomas@nbs.nhs.uk
authors.forename: R J
authors.initials: RJ
authors.lastname: Lomas
authors.forename: P D F
authors.initials: PD
authors.lastname: Dodd
authors.forename: P
authors.initials: P
authors.lastname: Rooney
authors.forename: D E
authors.initials: DE
authors.lastname: Pegg
authors.forename: P A
authors.initials: PA
authors.lastname: Hogg
authors.forename: M E
authors.initials: ME
authors.lastname: Eagle
authors.forename: K E
authors.initials: KE
authors.lastname: Bennett
authors.forename: A
authors.initials: A
authors.lastname: Clarkson
authors.forename: J N
authors.initials: JN
authors.lastname: Kearney
mesh_terms: D000328:Adult; D064591:Allografts / Q000502:physiology*; D001696:Biomechanical Phenomena; D002460:Cell Line; D015925:Cryopreservation; D005260:Female; D005263:Femoral Artery / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D011312:Pressure; D012015:Reference Standards; D015203:Reproducibility of Results; D013537:Sutures; D013718:Tensile Strength; D014015:Tissue Banks / Q000592:standards*; D014021:Tissue Preservation / Q000379:methods*; D055815:Young Adult