title: Expression of recombinant HA1 protein for specific detection of influenza A/H1N1/2009 antibodies in human serum.
abstract: The hemagglutinin genes (HA1 subunit) from human and animal 2009 pandemic H1N1 virus isolates were expressed with a baculovirus vector. Recombinant HA1 (rHA1) protein-based ELISA was evaluated for detection of specific influenza A(H1N1)pdm09 antibodies in serum samples from vaccinated humans. It was found that rHA1 ELISA consistently differentiated between antibodies recognizing the seasonal influenza H1N1 and pdm09 viruses, with a concordance of 94% as compared to the hemagglutination inhibition test. This study suggests the utility of rHA1 ELISA in serosurveillance.
authors.affiliation: National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, R3E 3M4, Canada.
authors.forename: Lizhong
authors.initials: L
authors.lastname: Luo
authors.forename: Krista
authors.initials: K
authors.lastname: Nishi
authors.forename: Erin
authors.initials: E
authors.lastname: Macleod
authors.forename: Marta I
authors.initials: MI
authors.lastname: Sabara
authors.forename: Brenda L
authors.initials: BL
authors.lastname: Coleman
authors.forename: Jonathan B
authors.initials: JB
authors.lastname: Gubbay
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000235:genetics; D016367:Baculoviridae / Q000235:genetics; D019411:Clinical Laboratory Techniques / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015870:Gene Expression; D005822:Genetic Vectors; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology; D009976:Orthomyxoviridae Infections / Q000175:diagnosis* / Q000821:virology; D011994:Recombinant Proteins / Q000235:genetics; D012680:Sensitivity and Specificity; D044967:Serum / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011994:Recombinant Proteins