title: Rhizobium qilianshanense sp. nov., a novel species isolated from root nodule of Oxytropis ochrocephala Bunge in China.
abstract: During a study of the diversity and phylogeny of rhizobia isolated from root nodules of Oxytropis ochrocephala grown in the northwest of China, four strains were classified in the genus Rhizobium on the basis of their 16S rRNA gene sequences. These strains have identical 16S rRNA gene sequences, which showed a mean similarity of 94.4 % with the most closely related species, Rhizobium oryzae. Analysis of recA and glnA sequences showed that these strains have less than 88.1 and 88.7 % similarity with the defined species of Rhizobium, respectively. The genetic diversity revealed by ERIC-PCR fingerprinting indicated that the isolates correspond to different strains. Strain CCNWQLS01(T) contains Q-10 as the predominant ubiquinone. The major fatty acids were identified as feature 8 (C18: 1ω7c and/or C18: 1ω6c; 67.2 %). Therefore, a novel species Rhizobium qilianshanense sp. nov. is proposed, and CCNWQLS01(T) (= ACCC 05747(T) = JCM 18337(T)) is designated as the type strain.
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming On the Loess Plateau, College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zheng Shan
authors.initials: ZS
authors.lastname: Deng
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.forename: Xiu Li
authors.initials: XL
authors.lastname: Wei
authors.forename: Ge Hong
authors.initials: GH
authors.lastname: Wei
mesh_terms: D015373:Bacterial Typing Techniques; D002681:China; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005974:Glutamate-Ammonia Ligase / Q000235:genetics; D008969:Molecular Sequence Data; D058889:Molecular Typing; D033021:Oxytropis / Q000382:microbiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics; D012231:Rhizobium / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D053204:Root Nodules, Plant / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D011938:Rec A Recombinases; C081173:glutamine synthetase I; D005974:Glutamate-Ammonia Ligase