title: Antiviral activity of punicalagin toward human enterovirus 71 in vitro and in vivo.
abstract: Human enterovirus 71 is one of the major causative agents of hand, foot and mouth disease in children and has caused mortalities in large-scale outbreaks in the Asia-Pacific region in recent years. No vaccine or antiviral therapy is available currently in the clinic. In this work, we investigated the antiviral effect of punicalagin on enterovirus 71 both in vitro and in vivo. The results showed that punicalagin reduced the viral cytopathic effect on rhabdomyosarcoma cells with an IC₅₀) value of 15 μg/ml. Moreover, punicalagin treatment of mice challenged with a lethal dose of enterovirus 71 resulted in a reduction of mortality and relieved clinical symptoms by inhibiting viral replication. Our work suggested that punicalagin have the potential for further development as antiviral agents against enterovirus 71.
authors.affiliation: Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Institute of Laboratory Animal Science, CAMS & Comparative Medicine Centre, PUMC, Beijing 100021, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Yang
authors.forename: Jinghui
authors.initials: J
authors.lastname: Xiu
authors.forename: Lianfeng
authors.initials: L
authors.lastname: Zhang
authors.forename: Chuan
authors.initials: C
authors.lastname: Qin
authors.forename: Jiangning
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D029821:Enterovirus A, Human; D004769:Enterovirus Infections / Q000188:drug therapy* / Q000401:mortality / Q000821:virology; D005536:Foot-and-Mouth Disease / Q000188:drug therapy / Q000821:virology; D006801:Humans; D047348:Hydrolyzable Tannins / Q000494:pharmacology / Q000627:therapeutic use*; D029561:Lythraceae / Q000737:chemistry*; D051379:Mice; D008813:Mice, Inbred ICR; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D047348:Hydrolyzable Tannins; D010936:Plant Extracts; C115642:punicalagin