title: A metabonomic approach to the effect evaluation of treatment in patients infected with influenza A (H1N1).
abstract: The pandemic influenza A virus (H1N1) was transmitted to the human population since 2009, resulting in some consequences of viral pneumonia, respiratory failure, multiple organ failure and, most severely, death. In clinical practice, Chinese medicine possessed extensive experience for prevention and treatment of influenza, but its mechanism still remain unclear. In addition, the efficacy of combination therapy of Chinese and Western medicine was attractive, but not yet clear. In the present study, 131 patients from Guangzhou China referred for H1N1 virus mRNA testing for the evaluation of possible influenza A-infected were eligible for participation. A metabonomics study was carried out to explore the difference between before and after treatment in patients with H1N1 through Chinese and/or Western medicine. Results from metabolic profiling and biochemical detection indicated significant metabolic change in the arachidonic acid metabolic pathway. In the group of combination therapy of Chinese and western medicine, its efficacy was best and the potential biomarkers were significantly changed compared with untreated state. Those results indicated that the potential metabolic biomarker could be supplemented with biochemical detection to obtain more precise diagnosis for H1N1 infection.
  
  
  BACKGROUND
  Clinical Trials. Gov No. 2008GL-50.
authors.affiliation: Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, PR China. luchuanjian888@vip.sina.com
authors.forename: Chuanjian
authors.initials: C
authors.lastname: Lu
authors.forename: Zhiting
authors.initials: Z
authors.lastname: Jiang
authors.forename: Xuemei
authors.initials: X
authors.lastname: Fan
authors.forename: Guiya
authors.initials: G
authors.lastname: Liao
authors.forename: Shasha
authors.initials: S
authors.lastname: Li
authors.forename: Chunxia
authors.initials: C
authors.lastname: He
authors.forename: Ling
authors.initials: L
authors.lastname: Han
authors.forename: Shijuan
authors.initials: S
authors.lastname: Luo
authors.forename: Yixin
authors.initials: Y
authors.lastname: Liu
authors.forename: Huangguo
authors.initials: H
authors.lastname: Lin
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.forename: Qionglin
authors.initials: Q
authors.lastname: Liang
authors.forename: Yiming
authors.initials: Y
authors.lastname: Wang
authors.forename: Guoan
authors.initials: G
authors.lastname: Luo
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D005260:Female; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D007251:Influenza, Human / Q000188:drug therapy* / Q000378:metabolism / Q000821:virology*; D008297:Male; D055432:Metabolomics / Q000379:methods*; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D015415:Biomarkers