title: [The purpose of clinical laboratory accreditation in transplantation medicine].
abstract: Although transplantation of solid organs has become a more standardized method of treatment, liver transplantation represents an exceptional multidisciplinary clinical procedure requiring understanding of specific pathophysiological changes that occur in the end stage of liver disease. Liver transplantation has been performed at Merkur University Hospital since 1998, with 360 transplantations performed to date. The most common indications are alcohol liver disease, cirrhosis caused by hepatitis B and C virus, hepatocellular carcinoma and cryptogenetic liver cirrhosis. Laboratory tests required for liver transplantation are performed at Department of Clinical Chemistry, Merkur University Hospital, accredited according to ISO 15189 in 2007 for the areas of clinical chemistry, laboratory hematology and coagulation, laboratory immunology-cell immunophenotyping, and molecular diagnosis. The complexity of liver transplant patients requires constant interaction between the anesthesiologist team and clinical laboratory, which has to ensure fast and efficient intraoperative monitoring of biochemical and liver profile: electrolytes and acid-base status, complete blood count, coagulation profile and monitoring of graft function according to the individual patient's health status. Dynamics of intraoperative changes is measured in whole arterial blood samples on a Nova Biomedical Stat Profile Critical Care Xpress mobile acid-base analyzer. Frequent monitoring of ionized calcium and magnesium levels is very important because of citrated blood transfusion and for appropriate therapeutic procedure. During anhepatic stage, there is a progressive increase in lactate level concentration. After reperfusion, a rapid increase in lactate clearance is an excellent indicator of stable graft initial function and its adequate size. During the transplantation procedure, there is usually a biphasic acid-base disturbance characterized by metabolic acidosis and then by metabolic alkalosis. The loss of base equivalents starts during the dissection stage and accelerates during the anhepatic stage. Fast and efficient intraoperative monitoring of hematological tests and coagulation status is of great help in detecting the cause of possible hemorrhage and consequential complications during transplantation procedure. The possibility of organ and tissue transplantation mostly depends on well regulated international cooperation in the areas of donating, transplanting and exchange of required organs and tissues, while laboratory test results must be comparable regardless of their geographical area, methodology employed or analytical equipment used, which is mainly warranted through accreditation according to the international ISO 15189 standard.
authors.affiliation: Merkur University Hospital, Institute of Clinical Chemistry and Laboratory Medicine, Zagreb, Croatia. zlata.mestri@gmail.com
authors.forename: Zlata
authors.initials: Z
authors.lastname: Flegar-Mestrić
authors.forename: Aida
authors.initials: A
authors.lastname: Nazor
authors.forename: Sonja
authors.initials: S
authors.lastname: Perkov
authors.forename: Branka
authors.initials: B
authors.lastname: Surina
authors.forename: Zoran
authors.initials: Z
authors.lastname: Siftar
authors.forename: Ivan
authors.initials: I
authors.lastname: Ozvald
authors.forename: Zeljko
authors.initials: Z
authors.lastname: Vidas
mesh_terms: D000068:Accreditation; D006403:Hematologic Tests; D006801:Humans; D007159:Immunologic Tests; D007755:Laboratories, Hospital; D016031:Liver Transplantation / Q000502:physiology; D008875:Middle Aged