title: Protein kinase C inhibitor BIM suspended TRPV1 effect on mu-opioid receptor.
abstract: The purpose of the present study was to elucidate the role of protein kinase A and C in the mechanism of capsaicin inhibition on mu-opiate receptors. H89, a protein kinase A inhibitor and BIM (bisindolylmaleimide), a protein kinase C inhibitor were used for this purpose. BIM suspended the inhibition of capsaicin in endomorphin-1 competition binding. The addition of BIM alone had no effect itself on this reaction. H89 however, exerted a strong inhibitory effect on the endomorphin-1 binding. We can conclude that protein kinase C certainly plays a role in the inhibition of capsaicin. The role of protein kinase A in this reaction could not be established, owing to the blocking effect of H89 on the mu-opioid receptors.
authors.affiliation: Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, H-6726 Szeged Temesvari Krt 62, Hungary.
authors.forename: Mária
authors.initials: M
authors.lastname: Wollemann
authors.forename: Fanni
authors.initials: F
authors.lastname: Tóth
authors.forename: Sándor
authors.initials: S
authors.lastname: Benyhe
mesh_terms: D000701:Analgesics, Opioid / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000648:ultrastructure; D002211:Capsaicin / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology*; D008297:Male; D008301:Maleimides / Q000494:pharmacology*; D009842:Oligopeptides / Q000494:pharmacology; D011869:Radioligand Assay; D051381:Rats; D017208:Rats, Wistar; D017450:Receptors, Opioid, mu / Q000378:metabolism*; D018689:Sensory System Agents / Q000494:pharmacology; D050916:TRPV Cation Channels / Q000378:metabolism*
chemical_list: D000701:Analgesics, Opioid; D004791:Enzyme Inhibitors; D007211:Indoles; D008301:Maleimides; D009842:Oligopeptides; D017450:Receptors, Opioid, mu; D018689:Sensory System Agents; D050916:TRPV Cation Channels; C105293:endomorphin 1; C088060:bisindolylmaleimide; D002211:Capsaicin