title: Cloning of galactinol synthase gene from Ammopiptanthus mongolicus and its expression in transgenic Photinia serrulata plants.
abstract: A cold induced galactinol synthase gene (AmGS) and its promoter sequence were identified and cloned from the cold-tolerant tree Ammopiptanthus mongolicus by using cDNA-AFLP, RACE-PCR and TAIL-PCR strategies combined with its expression pattern analysis after cold inducing treatment. Accession number of the AmGS gene in GenBank is DQ519361. The open reading frame (ORF) region of the AmGS gene is 987 nucleotides encoding for 328 amino acid residues and a stop codon. The genomic DNA sequence of AmGS gene contains 3 exons and 2 introns. Moreover, a variety of temporal gene expression patterns of AmGS was detected, which revealed the up-regulation of AmGS gene in stresses of cold, ABA and others. Then the AmGS gene was transformed into Photinia serrulata tree by Agrobacterium-mediated transformation, and the transgenic plants exhibited higher cold-tolerance comparing with non-transformed plants.
authors.affiliation: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Song
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Manli
authors.initials: M
authors.lastname: Weng
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Huang
authors.forename: Lei
authors.initials: L
authors.lastname: Luo
authors.forename: Pengxiu
authors.initials: P
authors.lastname: Cao
authors.forename: Haiwei
authors.initials: H
authors.lastname: Sun
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Jinhong
authors.initials: J
authors.lastname: Zhao
authors.forename: Dianqi
authors.initials: D
authors.lastname: Feng
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003080:Cold Temperature; D005091:Exons; D007887:Fabaceae / Q000235:genetics*; D005700:Galactosyltransferases / Q000096:biosynthesis / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D007438:Introns; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D046936:Photinia / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism
chemical_list: D000040:Abscisic Acid; D005700:Galactosyltransferases; C096645:inositol 1-alpha-galactosyltransferase