title: Cloning, purification and preliminary X-ray crystallographic analysis of the OmpA-like domain of peptidoglycan-associated lipoprotein from Acinetobacter baumannii.
abstract: Peptidoglycan-associated lipoprotein (Pal) is one component of the Tol-Pal system that is involved in maintaining the integrity and stability of the outer membrane. The C-terminal OmpA-like domain of Pal interacts noncovalently with peptidoglycan. In this study, the OmpA-like domain of Pal from Acinetobacter baumannii was overexpressed in Escherichia coli strain BL21 (DE3), purified and crystallized using the vapour-diffusion method. A native crystal diffracted to 1.4 Å resolution and belonged to space group P6(1) or P6(5), with unit-cell parameters a=b=72.58, c=44.65 Å, a calculated Matthews coefficient of 2.64 Å3 Da(-1) and one molecule per asymmetric unit.
authors.affiliation: Division of Magnetic Resonance Research, Korea Basic Science Institute, 804-1 Yangcheong-ri, Ochang, Chungbuk 363-883, Republic of Korea.
authors.forename: Jung Hyun
authors.initials: JH
authors.lastname: Song
authors.forename: Woo Cheol
authors.initials: WC
authors.lastname: Lee
authors.forename: Jeong Soon
authors.initials: JS
authors.lastname: Park
authors.forename: Seung Il
authors.initials: SI
authors.lastname: Kim
authors.forename: Je Chul
authors.initials: JC
authors.lastname: Lee
authors.forename: Chaejoon
authors.initials: C
authors.lastname: Cheong
authors.forename: Hye-Yeon
authors.initials: HY
authors.lastname: Kim
mesh_terms: D040981:Acinetobacter baumannii / Q000737:chemistry*; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004926:Escherichia coli; D008074:Lipoproteins / Q000737:chemistry* / Q000302:isolation & purification; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid
chemical_list: D001425:Bacterial Outer Membrane Proteins; D008074:Lipoproteins; C078167:OMPA outer membrane proteins