title: Long-term MRI tracking of dual-labeled adipose-derived stem cells homing into mouse carotid artery injury.
abstract: BACKGROUND
  Stem cell therapy has shown great promise for regenerative repair of injured or diseased tissues. Adipose-derived stem cells (ADSCs) have become increasingly attractive candidates for cellular therapy. Magnetic resonance imaging has been proven to be effective in tracking magnetic-labeled cells and evaluating their clinical relevance after cell transplantation. This study investigated the feasibility of imaging green fluorescent protein-expressing ADSCs (GFP-ADSCs) labeled with superparamagnetic iron oxide particles, and tracked them in vivo with noninvasive magnetic resonance imaging after cell transplantation in a model of mouse carotid artery injury.
  
  
  METHODS
  GFP-ADSCs were isolated from the adipose tissues of GFP mice and labeled with superparamagnetic iron oxide particles. Intracellular stability, proliferation, and viability of the labeled cells were evaluated in vitro. Next, the cells were transplanted into a mouse carotid artery injury model. Clinical 3 T magnetic resonance imaging was performed immediately before and 1, 3, 7, 14, 21, and 30 days after cell transplantation. Prussian blue staining and histological analysis were performed 7 and 30 days after transplantation.
  
  
  RESULTS
  GFP-ADSCs were found to be efficiently labeled with superparamagnetic iron oxide particles, with no effect on viability and proliferation. Homing of the labeled cells into the injured carotid artery tissue could be monitored by magnetic resonance imaging.
  
  
  CONCLUSIONS
  Magnetically labeled ADSCs with expression of GFP can home into sites of vascular injury, and may provide new insights into understanding of cell-based therapy for cardiovascular lesions.
authors.affiliation: Department of Vascular Surgery, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai, China.
authors.forename: Jin-Bao
authors.initials: JB
authors.lastname: Qin
authors.forename: Kang-An
authors.initials: KA
authors.lastname: Li
authors.forename: Xiang-Xiang
authors.initials: XX
authors.lastname: Li
authors.forename: Qing-Song
authors.initials: QS
authors.lastname: Xie
authors.forename: Jia-Ying
authors.initials: JY
authors.lastname: Lin
authors.forename: Kai-Chuang
authors.initials: KC
authors.lastname: Ye
authors.forename: Mi-Er
authors.initials: ME
authors.lastname: Jiang
authors.forename: Gui-Xiang
authors.initials: GX
authors.lastname: Zhang
authors.forename: Xin-Wu
authors.initials: XW
authors.lastname: Lu
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D020212:Carotid Artery Injuries / Q000473:pathology* / Q000601:surgery*; D002454:Cell Differentiation; D058948:Cell Tracking / Q000379:methods*; D002478:Cells, Cultured; D003287:Contrast Media; D003911:Dextrans; D049452:Green Fluorescent Proteins; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D013194:Staining and Labeling; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology*; D025321:Surgery, Computer-Assisted / Q000379:methods; D016896:Treatment Outcome
chemical_list: D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; C097921:ferumoxtran-10; D049452:Green Fluorescent Proteins
keywords: adipose-derived stem cells; carotid artery injury; cell therapy; iron oxide particles; magnetic resonance imaging