title: Polythiophene synthesis coupled to quartz crystal microbalance and Raman spectroscopy for detecting bacteria.
abstract: A simple electrochemical procedure was used for the synthesis of a polythiophene containing para-benzenesulfonyl chloride groups. The obtained polymer was shown to be very reactive and directly able to covalently bind nucleophile biomolecules. Protein A and a specific antibody were then successively immobilized on the conductive polymer through a covalent bonding of Protein A with the as-prepared linker for bacteria trapping purpose. All reactions were controlled in situ by cyclic voltammetry, quartz crystal microbalance and Raman spectroscopy. The results were compared to those previously obtained on gold surface modified with the same chemical linker. The conductive polymer led to a very high rate of antibody recognition compared to the gold surface and to literature, probably due to a large available surface obtained after polymerization. One example of pathogenic bacteria "Salmonella enterica paratyphi" detection was successfully tested on the substrates. The presented results are promising for the future design of simple and inexpensive immunocapture-based sensors.
authors.affiliation: LUNAM Université, Institut des Molécules et Matériaux du Mans (IMMM), UMR CNRS 6283, Université du Maine, Avenue Olivier Messiaen, 72085 Le Mans Cedex, France.
authors.forename: R P
authors.initials: RP
authors.lastname: Kengne-Momo
authors.forename: F
authors.initials: F
authors.lastname: Lagarde
authors.forename: Ph
authors.initials: P
authors.lastname: Daniel
authors.forename: J F
authors.initials: JF
authors.lastname: Pilard
authors.forename: M J
authors.initials: MJ
authors.lastname: Durand
authors.forename: G
authors.initials: G
authors.lastname: Thouand
mesh_terms: D000907:Antibodies, Bacterial / Q000737:chemistry / Q000378:metabolism; D055910:Antibodies, Immobilized / Q000737:chemistry / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques; D006046:Gold / Q000737:chemistry; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods*; D019779:Salmonella enterica / Q000737:chemistry* / Q000302:isolation & purification; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013205:Staphylococcal Protein A / Q000737:chemistry / Q000378:metabolism; D013876:Thiophenes / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D000907:Antibodies, Bacterial; D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D011108:Polymers; D013205:Staphylococcal Protein A; D013876:Thiophenes; C066730:polythiophene; D006046:Gold