title: Bisphenol A-glucuronide measurement in urine samples.
abstract: Bisphenol A (BPA), is one of the most abundant endocrine disruptors that are present in our environment, and has been repeatedly detected in most human biological samples. As it has been suggested that part of the BPA measured in human samples is due to contamination during samples collection or laboratory measurements, we have developed a specific radioimmunoassay for the measurement of BPA-glucuronide (BPA-G), the main endogenous metabolite of BPA in urine. We used a polyclonal anti-BPA antibody which has a 95% cross reactivity with BPA-G, and insignificant cross reactivity with most analogous BPA phenolic structures. To eliminate unconjugated BPA from urine samples, an extraction step with dichloromethane was required. The method proved to be valid, precise and accurate in the range of 0.05 μg/L to 5 μg/L. With this method, we measured BPA-G in 163 urine samples from a hospital population. We detected BPA-G in all samples, with mean values of 4.64 μg/L. In conclusion, the present radioimmunoassay is a useful tool for the screening of BPA exposure in human populations encompassing the problem of eventual contamination from laboratory manipulation.
authors.affiliation: Laboratoire d'Hormonologie, Centre de Biologie et de Pathologie Est, Groupement Hospitalier Est, Hospices Civils de Lyon, France. catherine.harthe@chu-lyon.fr
authors.forename: Catherine
authors.initials: C
authors.lastname: Harthé
authors.forename: Sabina
authors.initials: S
authors.lastname: Rinaldi
authors.forename: David
authors.initials: D
authors.lastname: Achaintre
authors.forename: Marc Rolland
authors.initials: MR
authors.lastname: de Ravel
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Mappus
authors.forename: Michel
authors.initials: M
authors.lastname: Pugeat
authors.forename: Henri
authors.initials: H
authors.lastname: Déchaud
mesh_terms: D001559:Benzhydryl Compounds / Q000737:chemistry* / Q000652:urine*; D052244:Endocrine Disruptors / Q000737:chemistry* / Q000652:urine*; D004781:Environmental Exposure / Q000032:analysis; D020719:Glucuronides / Q000737:chemistry*; D006801:Humans; D010636:Phenols / Q000737:chemistry* / Q000652:urine*; D011863:Radioimmunoassay; D013997:Time Factors; D016482:Urinalysis / Q000379:methods*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D020719:Glucuronides; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds