title: An investigation on the cytotoxicity and caspase-mediated apoptotic effect of biologically synthesized silver nanoparticles using Podophyllum hexandrum on human cervical carcinoma cells.
abstract: Now-a-days synthesis and characterization of silver nanoparticles (AgNPs) through biological entity is quite interesting to employ AgNPs for various biomedical applications in general and treatment of cancer in particular. This paper presents the green synthesis of AgNPs using leaf extract of Podophyllum hexandrum Royle and optimized with various parameters such as pH, temperature, reaction time, volume of extract and metal ion concentration for synthesis of AgNPs. TEM, XRD and FTIR were adopted for characterization. The synthesized nanoparticles were found to be spherical shaped with average size of 14 nm. Effects of AgNPs were analyzed against human cervical carcinoma cells by MTT Assay, quantification of ROS, RT-PCR and western blotting techniques. The overall result indicates that AgNPs can selectively inhibit the cellular mechanism of HeLa by DNA damage and caspase mediated cell death. This biological procedure for synthesis of AgNPs and selective inhibition of cancerous cells gives an alternative avenue to treat human cancer effectively.
authors.affiliation: Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tamil Nadu, India. jeymuruga@gmail.com
authors.forename: Murugaraj
authors.initials: M
authors.lastname: Jeyaraj
authors.forename: Manoharan
authors.initials: M
authors.lastname: Rajesh
authors.forename: Renganathan
authors.initials: R
authors.lastname: Arun
authors.forename: Davoodbasha
authors.initials: D
authors.lastname: MubarakAli
authors.forename: Gnanasekar
authors.initials: G
authors.lastname: Sathishkumar
authors.forename: Ganeshan
authors.initials: G
authors.lastname: Sivanandhan
authors.forename: Gnanajothi Kapil
authors.initials: GK
authors.lastname: Dev
authors.forename: Markandan
authors.initials: M
authors.lastname: Manickavasagam
authors.forename: Kumpati
authors.initials: K
authors.lastname: Premkumar
authors.forename: Nooruddin
authors.initials: N
authors.lastname: Thajuddin
authors.forename: Andy
authors.initials: A
authors.lastname: Ganapathi
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D020169:Caspases / Q000378:metabolism*; D045744:Cell Line, Tumor; D004249:DNA Damage / Q000187:drug effects; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000009:adverse effects / Q000737:chemistry / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D010936:Plant Extracts / Q000737:chemistry*; D011035:Podophyllum / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012834:Silver / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D010936:Plant Extracts; D012834:Silver; D020169:Caspases