title: Sphingoid bases are taken up by Escherichia coli and Staphylococcus aureus and induce ultrastructural damage.
abstract: Sphingoid bases found in the outer layers of the skin exhibit antimicrobial activity against gram-positive and gram-negative bacteria. We investigated the uptake of several sphingoid bases by Escherichia coli and Staphylococcus aureus, and assessed subsequent ultrastructural damage. E. coli and S. aureus were incubated with D-sphingosine, dihydrosphingosine, or phytosphingosine at ten times their MIC for 0.5 and 4 h, respectively, to kill 50% of viable bacteria. Treated bacterial cells were immediately prepared for SEM, TEM, and analyzed for lipid content by QTLC. E. coli and S. aureus treated with sphingoid bases were distorted and their surfaces were concave and rugate. Significant differences were observed in the visual surface area relative to controls for both E. coli and S. aureus when treated with dihydrosphingosine and sphingosine (p < 0.0001) but not phytosphingosine. While sphingoid base-treated S. aureus exhibited disruption and loss of cell wall and membrane, E. coli cytoplasmic membranes appeared intact and the outer envelope uncompromised. Both E. coli and S. aureus cells contained unique internal inclusion bodies, likely associated with cell death. QTLC demonstrated extensive uptake of sphingoid bases by the bacteria. Hence, sphingoid bases induce both extracellular and intracellular damage and cause intracellular inclusions that may reflect lipid uptake.
authors.affiliation: Dows Institute for Dental Research, Iowa City, Iowa, USA.
authors.forename: C L
authors.initials: CL
authors.lastname: Fischer
authors.forename: K S
authors.initials: KS
authors.lastname: Walters
authors.forename: D R
authors.initials: DR
authors.lastname: Drake
authors.forename: D R
authors.initials: DR
authors.lastname: Blanchette
authors.forename: D V
authors.initials: DV
authors.lastname: Dawson
authors.forename: K A
authors.initials: KA
authors.lastname: Brogden
authors.forename: P W
authors.initials: PW
authors.lastname: Wertz
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D000900:Anti-Bacterial Agents; C012491:phytosphingosine; D013110:Sphingosine; C005682:safingol