title: α-Glucosidase inhibitor from Buthus martensi Karsch.
abstract: A bioassay-guided fractionation of an ethanol extract of Buthus martensi Karsch led to the isolation of a potent α-glucosidase inhibitor (compound S). The structure was elucidated as a novel β-carboline glucoalkaloid, harmanyl β-d-glucopyranoside, on the basis of spectral data, including (1)H NMR, (13)C NMR, (1)H-(1)H COSY, NOESY, and HMBC. Compound S showed potent inhibitory activity against α-glucosidase, with an IC(50) value of 24 μM. A Lineweaver-Burk plot indicated that its inhibition of α-glucosidase was uncompetitive, with a Ki value of 16.1 μM.
authors.affiliation: Department of Chemical and Biological Engineering, Seokyeong University, Seoul 136-704, Republic of Korea. sdkim@skuniv.ac.kr
authors.forename: Shin-Duk
authors.initials: SD
authors.lastname: Kim
mesh_terms: D000818:Animals; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000378:metabolism; D065089:Glycoside Hydrolase Inhibitors; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D012605:Scorpions / Q000737:chemistry* / Q000378:metabolism; D000520:alpha-Glucosidases / Q000737:chemistry
chemical_list: D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D000520:alpha-Glucosidases