title: Mouse embryonic head as a site for hematopoietic stem cell development.
abstract: In the mouse embryo, the aorta-gonad-mesonephros (AGM) region is considered to be the sole location for intraembryonic emergence of hematopoietic stem cells (HSCs). Here we report that, in parallel to the AGM region, the E10.5-E11.5 mouse head harbors bona fide HSCs, as defined by long-term, high-level, multilineage reconstitution and self-renewal capacity in adult recipients, before HSCs enter the circulation. The presence of hemogenesis in the midgestation head is indicated by the appearance of intravascular cluster cells and the blood-forming capacity of a sorted endothelial cell population. In addition, lineage tracing via an inducible VE-cadherin-Cre transgene demonstrates the hemogenic capacity of head endothelium. Most importantly, a spatially restricted lineage labeling system reveals the physiological contribution of cerebrovascular endothelium to postnatal HSCs and multilineage hematopoiesis. We conclude that the mouse embryonic head is a previously unappreciated site for HSC emergence within the developing embryo.
authors.affiliation: 307-Ivy Translational Medicine Center, Laboratory of Oncology, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Zhuan
authors.initials: Z
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Lan
authors.forename: Wenyan
authors.initials: W
authors.lastname: He
authors.forename: Dongbo
authors.initials: D
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Fan
authors.initials: F
authors.lastname: Zhou
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Huayan
authors.initials: H
authors.lastname: Sun
authors.forename: Xianda
authors.initials: X
authors.lastname: Chen
authors.forename: Chunhong
authors.initials: C
authors.lastname: Xu
authors.forename: Sha
authors.initials: S
authors.lastname: Li
authors.forename: Yakun
authors.initials: Y
authors.lastname: Pang
authors.forename: Guangzhou
authors.initials: G
authors.lastname: Zhang
authors.forename: Liping
authors.initials: L
authors.lastname: Yang
authors.forename: Lingling
authors.initials: L
authors.lastname: Zhu
authors.forename: Ming
authors.initials: M
authors.lastname: Fan
authors.forename: Aijia
authors.initials: A
authors.lastname: Shang
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Ju
authors.forename: Lingfei
authors.initials: L
authors.lastname: Luo
authors.forename: Yuqiang
authors.initials: Y
authors.lastname: Ding
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.forename: Weiping
authors.initials: W
authors.lastname: Yuan
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D001011:Aorta / Q000166:cytology / Q000196:embryology; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D004622:Embryo, Mammalian / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000196:embryology; D006066:Gonads / Q000166:cytology / Q000196:embryology; D006257:Head / Q000098:blood supply / Q000196:embryology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D008650:Mesonephros / Q000166:cytology / Q000196:embryology; D051379:Mice; D019076:Transgenes; D000099152:Cadherin 5
chemical_list: D015703:Antigens, CD; D015820:Cadherins; D000099152:Cadherin 5