title: Tissue-specific distribution of secondary metabolites in rapeseed (Brassica napus L.).
abstract: Four different parts, hypocotyl and radicle (HR), inner cotyledon (IC), outer cotyledon (OC), seed coat and endosperm (SE), were sampled from mature rapeseed (Brassica napus L.) by laser microdissection. Subsequently, major secondary metabolites, glucosinolates and sinapine, as well as three minor ones, a cyclic spermidine conjugate and two flavonoids, representing different compound categories, were qualified and quantified in dissected samples by high-performance liquid chromatography with diode array detection and mass spectrometry. No qualitative and quantitative difference of glucosinolates and sinapine was detected in embryo tissues (HR, IC and OC). On the other hand, the three minor compounds were observed to be distributed unevenly in different rapeseed tissues. The hypothetic biological functions of the distribution patterns of different secondary metabolites in rapeseed are discussed.
authors.affiliation: Max Planck Institute for Chemical Ecology, Jena, Germany.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Fang
authors.forename: Michael
authors.initials: M
authors.lastname: Reichelt
authors.forename: William
authors.initials: W
authors.lastname: Hidalgo
authors.forename: Sara
authors.initials: S
authors.lastname: Agnolet
authors.forename: Bernd
authors.initials: B
authors.lastname: Schneider
mesh_terms: D029688:Brassica napus / Q000378:metabolism*; D002794:Choline / Q000031:analogs & derivatives / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D018548:Cotyledon / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005961:Glucosinolates / Q000378:metabolism; D018546:Hypocotyl / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods; D008954:Models, Biological; D008956:Models, Chemical; D019800:Phenol / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D014018:Tissue Distribution
chemical_list: D005961:Glucosinolates; C005685:sinapine; D019800:Phenol; D002794:Choline