title: Impurity profiling of capreomycin using dual liquid chromatography coupled to mass spectrometry.
abstract: The characterization of unknown (UNK) impurities in capreomycin (CMN) using liquid chromatography coupled to mass spectrometry (LC/MS) has been described. The ion-pair liquid chromatography method coupled to ultraviolet detection (LC-UV) described by Mallampati et al. was used for the separation of CMN from its related substances. As the method uses non-volatile reagents it could not be directly coupled to mass spectrometry (MS) for impurity characterization. So, these UNK impurities were collected and desalted before sending to MS for structural characterization. As no information about the fragmentation of the main components of CMN, except for CMN IB, was available in the literature, they were studied first. Next, the structures of the impurities were deduced by comparing their fragmentation to that of the main components of CMN. Fourteen UNK impurities that were never described before, were (partly) characterized.
authors.affiliation: Laboratory for Pharmaceutical Analysis, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, Herestraat 49, B-3000 Leuven, Belgium.
authors.forename: Shruti
authors.initials: S
authors.lastname: Chopra
authors.forename: Murali
authors.initials: M
authors.lastname: Pendela
authors.forename: Jos
authors.initials: J
authors.lastname: Hoogmartens
authors.forename: Ann
authors.initials: A
authors.lastname: Van Schepdael
authors.forename: Erwin
authors.initials: E
authors.lastname: Adams
mesh_terms: D002207:Capreomycin / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D004340:Drug Contamination; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D002207:Capreomycin