title: DNA vaccine encoding the major virulence factors of Shiga toxin type 2e (Stx2e)-expressing Escherichia coli induces protection in mice.
abstract: Piglet edema disease is found worldwide and has historically been treated with antibiotics. However, no commercial vaccines are available for its prevention. In this study, the two major virulence factors of Shiga-toxigenic Escherichia coli (Stx2eB and FedF) were cloned to a pcDNA6.0 plasmid to develop a novel DNA vaccine against piglet edema disease. In animal trial in mouse model, the antibody titer, mortality, serum cytokine levels (interleukin-1 beta, interleukin-6, tumor necrosis factor alpha and C-reactive protein), serum malondialdehyde level and serum total superoxide dismutase activity were measured to validate the effectiveness of the DNA vaccine. The results show that Stx2eB and FedF at least partially protect against edema disease and FedF is more effective than Stx2eB. Co-immunization with both Stx2eB and FedF is most effective for protecting mice from a subsequent challenge with E. coli O139 (which is known to cause edema disease in pigs).
authors.affiliation: Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125, China.
authors.forename: Wenkai
authors.initials: W
authors.lastname: Ren
authors.forename: Rui
authors.initials: R
authors.lastname: Yu
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.forename: Nengzhang
authors.initials: N
authors.lastname: Li
authors.forename: Yuanyi
authors.initials: Y
authors.lastname: Peng
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Wu
authors.forename: Yulong
authors.initials: Y
authors.lastname: Yin
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Li
authors.forename: Andrew A
authors.initials: AA
authors.lastname: Fatufe
authors.forename: Teijun
authors.initials: T
authors.lastname: Li
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D002097:C-Reactive Protein / Q000276:immunology; D002460:Cell Line; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology / Q000517:prevention & control*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000276:immunology; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D053583:Interleukin-1beta / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D051379:Mice; D011897:Random Allocation; D022641:Shiga Toxin 2 / Q000235:genetics / Q000276:immunology*; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D037521:Virulence Factors / Q000235:genetics* / Q000276:immunology*
chemical_list: D018829:Adhesins, Bacterial; D000906:Antibodies; D001428:Bacterial Vaccines; D029968:Escherichia coli Proteins; C440653:FedF protein, E coli; D053583:Interleukin-1beta; D015850:Interleukin-6; D022641:Shiga Toxin 2; D014409:Tumor Necrosis Factor-alpha; D019444:Vaccines, DNA; D037521:Virulence Factors; D002097:C-Reactive Protein