title: Oxidation matters: the ubiquitin proteasome system connects innate immune mechanisms with MHC class I antigen presentation.
abstract: During innate immune responses the delicate balance of protein synthesis, quality control and degradation is severely challenged by production of radicals and/or the massive synthesis of pathogen proteins. The regulated degradation of ubiquitin-tagged proteins by the ubiquitin proteasome system (UPS) represents one major pathway for the maintenance of cellular proteostasis and regulatory processes under these conditions. In addition, MHC class I antigen presentation is strictly dependent on an appropriate peptide supply by the UPS to efficiently prime CD8(+) T cells and to initiate an adaptive immune response. We here discuss recent efforts in defining the link between innate immune mechanisms like cytokine and ROS production, the induction of an efficient adaptive immune response and the specific involvement of the UPS therein. Cytokines and/or infections induce translation and the production of free radicals, which in turn confer oxidative damage to nascent as well as folded proteins. In parallel, the same signaling cascades are able to accelerate the protein turnover by the concomitantly induced ubiquitin conjugation and degradation of such damaged polypeptides by immunoproteasomes. The ability of immunoproteasomes to efficiently degrade such oxidant-damaged ubiquitylated proteins protects cells from accumulating toxic ubiquitin-rich aggregates. At the same time, this innate immune mechanism facilitates a sufficient peptide supply for MHC class I antigen presentation and connects it to initiation of adaptive immunity.
authors.affiliation: Charité-Universitätsmedizin Berlin, Charité CrossOver, Institut für Biochemie CCM, Berlin, Germany.
authors.forename: Annika
authors.initials: A
authors.lastname: Warnatsch
authors.forename: Theresa
authors.initials: T
authors.lastname: Bergann
authors.forename: Elke
authors.initials: E
authors.lastname: Krüger
mesh_terms: D056704:Adaptive Immunity; D017951:Antigen Presentation; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007113:Immunity, Innate; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*
chemical_list: D015395:Histocompatibility Antigens Class I; D017382:Reactive Oxygen Species; D044767:Ubiquitin-Protein Ligases