title: Systemic transport of Alfalfa mosaic virus can be mediated by the movement proteins of several viruses assigned to five genera of the 30K family.
abstract: We previously showed that the movement protein (MP) gene of Alfalfa mosaic virus (AMV) is functionally exchangeable for the cell-to-cell transport of the corresponding genes of Tobacco mosaic virus (TMV), Brome mosaic virus, Prunus necrotic ringspot virus, Cucumber mosaic virus and Cowpea mosaic virus. We have analysed the capacity of the heterologous MPs to systemically transport the corresponding chimeric AMV genome. All MPs were competent in systemic transport but required the fusion at their C terminus of the coat protein-interacting C-terminal 44 aa (A44) of the AMV MP. Except for the TMV MP, the presence of the hybrid virus in upper leaves correlated with the capacity to move locally. These results suggest that all the MPs assigned to the 30K superfamily should be exchangeable not only for local virus movement but also for systemic transport when the A44 fragment is present.
authors.affiliation: Embrapa Uva e Vinho, Rua Livramento, 515. Bento Gonçalves-RS, CEP 95700-000, Brazil.
authors.forename: Thor V M
authors.initials: TVM
authors.lastname: Fajardo
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-CSIC, Avenida de los Naranjos s/n, 46022 Valencia, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Peiró
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-CSIC, Avenida de los Naranjos s/n, 46022 Valencia, Spain.
authors.forename: Vicente
authors.initials: V
authors.lastname: Pallás
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-CSIC, Avenida de los Naranjos s/n, 46022 Valencia, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Sánchez-Navarro
mesh_terms: D017794:Alfalfa mosaic virus / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D049452:Green Fluorescent Proteins; D053830:Plant Viral Movement Proteins / Q000502:physiology*; D030821:Plants, Genetically Modified; D021381:Protein Transport; D012367:RNA, Viral / Q000235:genetics; D011994:Recombinant Proteins; D014026:Nicotiana / Q000235:genetics; D014779:Virus Replication
chemical_list: D053830:Plant Viral Movement Proteins; D012367:RNA, Viral; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins