title: Backbone and sterospecific methyl side chain resonance assignments of the homodimeric zinc sensor AdcR (32 kDa) in the apo- and Zn(II)-bound states.
abstract: Streptococcus pneumoniae adhesin competence repressor (AdcR) is a Zn(II)-dependent 32 kDa homodimer that controls the transcription of a zinc-specific ABC uptake system (AdcABC), three pneumococcal histidine triad proteins (PhtA, PhtD and PhtE), and an AdcA homolog AdcAII. AdcR is the first metal-dependent member of the MarR family of prokaryotic transcriptional repressors. Two-dimensional NMR studies reveal large changes in the spectrum upon Zn(II) binding. Near complete backbone and stereospecific methyl group resonance assignments for apo- and Zn(II)-AdcR are presented here.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington, IN, 47405-7102, USA.
authors.forename: Alfredo J
authors.initials: AJ
authors.lastname: Guerra
authors.forename: David P
authors.initials: DP
authors.lastname: Giedroc
mesh_terms: D000595:Amino Acid Sequence; D001059:Apoproteins / Q000737:chemistry / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D013237:Stereoisomerism; D013296:Streptococcus pneumoniae; D015032:Zinc / Q000378:metabolism*
chemical_list: D001059:Apoproteins; D001426:Bacterial Proteins; D015032:Zinc