title: Functional assay for shiga-like toxin via detection by antibody capture and multivalent galabiose binding.
abstract: A functional detection assay was developed for Escherichia coli secreted shiga-like toxin based on antibody capture and visualization with a multivalent galabiose ligand. It was possible to detect verotoxin in medically relevant E. coli samples in a dose dependent fashion. This method is a new step towards measuring functional protein levels in complex mixtures, which can be used for diagnostic purposes in a clinical setting.
authors.affiliation: Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, PO Box 80082, 3508 TB Utrecht, The Netherlands.
authors.forename: Hilde
authors.initials: H
authors.lastname: van Hattum
authors.forename: Kim
authors.initials: K
authors.lastname: van der Zwaluw
authors.forename: Gerben M
authors.initials: GM
authors.lastname: Visser
authors.forename: Jos
authors.initials: J
authors.lastname: van Putten
authors.forename: Rob
authors.initials: R
authors.lastname: Ruijtenbeek
authors.forename: Roland J
authors.initials: RJ
authors.lastname: Pieters
mesh_terms: D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D000937:Antigen-Antibody Reactions; D000942:Antigens, Bacterial / Q000032:analysis* / Q000737:chemistry* / Q000276:immunology; D001665:Binding Sites; D001710:Biotin / Q000737:chemistry; D004187:Disaccharides / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry; D008024:Ligands; D012710:Serum Albumin, Bovine / Q000737:chemistry; D022603:Shiga Toxins / Q000032:analysis* / Q000737:chemistry* / Q000276:immunology
chemical_list: D000906:Antibodies; D000942:Antigens, Bacterial; D004187:Disaccharides; D008024:Ligands; D022603:Shiga Toxins; C032103:4-O-alpha-D-galactopyranosyl-D-galactose; D012710:Serum Albumin, Bovine; D001710:Biotin