title: Making cRNA for microinjection and expression of fluorescently tagged proteins for live-cell imaging in oocytes.
abstract: Fluorescently tagged proteins have become a crucial weapon in the armory of a successful cell biology laboratory. This chapter describes how to produce cRNA coding for a fluorescently tagged protein of choice, such that it is suitable for microinjection and subsequent expression studies in live oocytes.
authors.affiliation: Institute for Cell and Molecular Biosciences, The Medical School, Newcastle University, Newcastle, UK. m.d.levasseur@ncl.ac.uk
authors.forename: Mark
authors.initials: M
authors.lastname: Levasseur
mesh_terms: D000818:Animals; D002470:Cell Survival; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics*; D008164:Luminescent Proteins / Q000235:genetics*; D008845:Microinjections / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D018075:RNA, Complementary / Q000008:administration & dosage / Q000235:genetics*; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D000097573:Red Fluorescent Protein
chemical_list: D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D018075:RNA, Complementary; D000097573:Red Fluorescent Protein