title: Crystal ball: fluorescence in situ hybridization in the age of super-resolution microscopy.
abstract: Super-resolution microscopy encompasses a suite of cutting edge microscopy methods able to surpass the resolution limits of light microscopy. The recent commercial availability of super-resolution microscopy is advancing many fields of biology. In this crystal ball forward look, we briefly examine the perspectives of combining super-resolution microscopy and fluorescence in situ hybridization (FISH). We strongly believe, based on first evidence presented here, that using super-resolution microscopy in environmental microbiology has the potential to reshape the way we analyze the results obtained with FISH, by improving both the localization and quantification of target molecules.
authors.affiliation: Max Planck Institute for Marine Microbiology, Celssiusstrasse 1, 28359 Bremen, Germany. CristinaMoraru@TheScientist.info
authors.forename: Cristina
authors.initials: C
authors.lastname: Moraru
authors.forename: Rudolf
authors.initials: R
authors.lastname: Amann
mesh_terms: D004783:Environmental Microbiology; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008829:Microbiology / Q000639:trends; D008853:Microscopy / Q000379:methods*