title: Differential expression profiles of microRNAs as potential biomarkers             for the early diagnosis of esophageal squamous cell carcinoma.
abstract: Esophageal squamous cell carcinoma (ESCC) is one of the most lethal malignancies             worldwide. To reduce the high morbidity and mortality of the disease, sensitive             and specific biomarkers for early detection are urgently needed. Tumor-specific             microRNAs (miRNAs) seem to be potential biomarkers for the early diagnosis and             treatment of cancer. In this study, differentially expressed miRNAs in tumor tissues             and adjacent non-tumor tissues were detected by miRNA microarrays. Stem-loop real-time             reverse transcription PCR was conducted to verify the candidate miRNAs discovered             by microarray analysis. The data showed that hsa-miR-338-3p, hsa-miR‑218 and hsa-miR-139-5p             were downregulated in tumor tissues compared with adjacent non-tumor tissues,             while hsa-miR‑183, hsa-miR-574-5p, hsa-miR-21* and hsa-miR‑601 were upregulated             in tumor tissues. Multiple regression analysis revealed the aberrant expression             of hsa-miR-338-3p, hsa‑miR-139-5p, hsa-miR‑574-5p and hsa-miR-601 increased the             risk of esophageal cancer. Furthermore, we found hsa-miR-21* was significantly             increased in heavy drinking patients. Therefore, there is a set of differentially             expressed miRNAs in esophageal cancer which may be associated with the incidence             and development of ESCC. Differential expression profiles of miRNAs in ESCC may             be promising biomarkers for the early screening of high-risk populations and early             detection.
authors.affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, Jiangsu 210009, PR China.
authors.forename: Miao
authors.initials: M
authors.lastname: Yang
authors.forename: Ran
authors.initials: R
authors.lastname: Liu
authors.forename: Jingyi
authors.initials: J
authors.lastname: Sheng
authors.forename: Juan
authors.initials: J
authors.lastname: Liao
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Enchun
authors.initials: E
authors.lastname: Pan
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.forename: Yuepu
authors.initials: Y
authors.lastname: Pu
authors.forename: Lihong
authors.initials: L
authors.lastname: Yin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000235:genetics; D042241:Early Diagnosis; D004938:Esophageal Neoplasms / Q000175:diagnosis* / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D012333:RNA, Messenger