title: Role of lumenal domain on intracellular localization of tobacco membrane-anchored prolyl 4-hydroxylase.
abstract: NtP4H1.1 is a Golgi-localizing type II integral membrane protein. Mutations in the cytoplasmic tail direct the protein to the endoplasmic reticulum (ER). We expressed a GFP fusion protein containing the mutant tail and the transmembrane region in tobacco BY-2 cells, and found that the protein localized in the Golgi. Therefore NtP4H1.1 contains multiple targeting information in different regions.
authors.affiliation: Laboratory of Plant Nutrition, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Munemasa
authors.forename: Akiko
authors.initials: A
authors.lastname: Suyama
authors.forename: Ken
authors.initials: K
authors.lastname: Matsuoka
mesh_terms: D002462:Cell Membrane / Q000201:enzymology*; D042541:Intracellular Space / Q000201:enzymology*; D011393:Procollagen-Proline Dioxygenase / Q000737:chemistry* / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D014026:Nicotiana / Q000166:cytology* / Q000201:enzymology*
chemical_list: D011393:Procollagen-Proline Dioxygenase