title: A novel electrochemiluminescence ethanol biosensor based on tris(2,2'-bipyridine) ruthenium (II) and alcohol dehydrogenase immobilized in graphene/bovine serum albumin composite film.
abstract: We developed a novel electrochemiluminescence (ECL) ethanol biosensor based on Ru(bpy)(3)(2+) and alcohol dehydrogenase (ADH) immobilized by graphene/bovine serum albumin composite film. The graphene film was directly formed on a glassy carbon electrode surface via an in situ reduction of graphene oxide (GO) and Ru(bpy)(3)(2+) was immobilized during its formation. The graphene film acted as both a decorating agent for immobilization of Ru(bpy)(3)(2+) and a matrix to immobilize bovine serum albumin (BSA), meanwhile BSA not only acted as a reductant to reduce GO, but also provided a friendly environment for ADH immobilization. Furthermore, ADH was separated from Ru(bpy)(3)(2+) by the electron-conductive graphene/BSA composite film to retain its enzymatic activity. The experimental results indicated that the biosensor had excellent electrochemical activity, ECL response to ethanol and stability. Such a design of Ru(bpy)(3)(2+)-graphene/BSA film to modify electrode holds a great promise as a new biocompatible platform for the development of enzyme-based ECL biosensors.
authors.affiliation: Department of Chemistry, Shantou University, Shantou, Guangdong 515063, China. whgao@stu.edu.cn
authors.forename: Wenhua
authors.initials: W
authors.lastname: Gao
authors.forename: Yunsheng
authors.initials: Y
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Xi
authors.forename: Shaoyu
authors.initials: S
authors.lastname: Lin
authors.forename: Yaowen
authors.initials: Y
authors.lastname: Chen
authors.forename: Yuejuan
authors.initials: Y
authors.lastname: Lin
authors.forename: Zhanguang
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D000431:Ethanol / Q000032:analysis* / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D008163:Luminescent Measurements / Q000295:instrumentation*; D015203:Reproducibility of Results; D012428:Ruthenium / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D012710:Serum Albumin, Bovine; D000431:Ethanol; D006108:Graphite; D012428:Ruthenium; D000426:Alcohol Dehydrogenase