title: The role of glutathione S-transferase and claudin-1 gene polymorphisms in contact sensitization: a cross-sectional study.
abstract: BACKGROUND
  Contact sensitization is frequent in the general population and arises from excessive or repeated skin exposure to chemicals and metals. However, little is known about its genetic susceptibility.
  
  
  OBJECTIVE
  To determine the role of polymorphisms of glutathione S-transferase (GST) genes and the claudin-1 gene (CLDN1) on the risk of contact sensitization, taking common filaggrin gene (FLG) mutations into account.
  
  
  METHODS
  In total, 3471 adult Danes from the general population were standard patch tested and filled out a questionnaire on their general health. They were genotyped for the following polymorphisms: GSTM1 and GSTT1 deletion, GSTP1 single nucleotide polymorphism (SNP) rs1695, four CLDN1 SNPs (rs893051, rs9290927, rs9290929 and rs17501010) and the FLG null mutations R501X and 2282del4.
  
  
  RESULTS
  In individuals without ear piercings, a higher prevalence of nickel sensitization was found in those with the minor allele of CLDN1 SNP rs9290927 (P(trend)=0·013). For CLDN1 rs17501010, contact sensitization to organic compounds was associated with the major allele (P(trend)=0·031). The risk pattern was also identified for self-reported nickel dermatitis (P(trend)=0·011). The fragrance sensitization prevalence differed in a pairwise comparison of the CLDN1 rs893051 SNP genotypes (P=0·022), with the minor allele being associated with a higher prevalence. The associations were confirmed in logistic regression analyses.
  
  
  CONCLUSIONS
  The CLDN1 polymorphisms rs9290927, rs893051 and rs17501010 were associated, respectively, with nickel contact sensitization in individuals without ear piercings, contact sensitization to fragrances, and with both organic compounds and nickel contact dermatitis. We could not find associations between GST gene polymorphisms and contact sensitization. FLG mutations did not affect the observed associations.
authors.affiliation: National Allergy Research Centre, Department of Dermato-Allergology, Copenhagen University Hospital Gentofte, Hellerup, Denmark. katrine.ross-hansen@regionh.dk
authors.forename: K
authors.initials: K
authors.lastname: Ross-Hansen
authors.forename: A
authors.initials: A
authors.lastname: Linneberg
authors.forename: J D
authors.initials: JD
authors.lastname: Johansen
authors.forename: L-G
authors.initials: LG
authors.lastname: Hersoug
authors.forename: C
authors.initials: C
authors.lastname: Brasch-Andersen
authors.forename: T
authors.initials: T
authors.lastname: Menné
authors.forename: J P
authors.initials: JP
authors.lastname: Thyssen
mesh_terms: D000328:Adult; D000485:Allergens / Q000235:genetics / Q000276:immunology; D062445:Claudin-1 / Q000235:genetics*; D003430:Cross-Sectional Studies; D003877:Dermatitis, Contact / Q000235:genetics*; D000091344:Filaggrin Proteins; D017353:Gene Deletion; D020022:Genetic Predisposition to Disease; D005838:Genotype; D051549:Glutathione S-Transferase pi / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D007381:Intermediate Filament Proteins / Q000235:genetics; D009154:Mutation / Q000235:genetics; D010328:Patch Tests; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D000485:Allergens; D062445:Claudin-1; D000091344:Filaggrin Proteins; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D007381:Intermediate Filament Proteins; C568108:CLDN1 protein, human; C000717249:FLG protein, human; C413545:glutathione S-transferase T1; C496556:GSTP1 protein, human