title: Use of poly(DL-lactide-ε-caprolactone) membranes and mesenchymal stem cells from the Wharton's jelly of the umbilical cord for promoting nerve regeneration in axonotmesis: in vitro and in vivo analysis.
abstract: Cellular systems implanted into an injured nerve may produce growth factors or extracellular matrix molecules, modulate the inflammatory process and eventually improve nerve regeneration. In the present study, we evaluated the therapeutic value of human umbilical cord matrix MSCs (HMSCs) on rat sciatic nerve after axonotmesis injury associated to Vivosorb® membrane. During HMSCs expansion and differentiation in neuroglial-like cells, the culture medium was collected at 48, 72 and 96 h for nuclear magnetic resonance (NMR) analysis in order to evaluate the metabolic profile. To correlate the HMSCs ability to differentiate and survival capacity in the presence of the Vivosorb® membrane, the [Ca(2+)]i of undifferentiated HMSCs or neuroglial-differentiated HMSCs was determined by the epifluorescence technique using the Fura-2AM probe. The Vivosorb® membrane proved to be adequate and used as scaffold associated with undifferentiated HMSCs or neuroglial-differentiated HMSCs. In vivo testing was carried out in adult rats where a sciatic nerve axonotmesis injury was treated with undifferentiated HMSCs or neuroglial differentiated HMSCs with or without the Vivosorb® membrane. Motor and sensory functional recovery was evaluated throughout a healing period of 12 weeks using sciatic functional index (SFI), extensor postural thrust (EPT), and withdrawal reflex latency (WRL). Stereological analysis was carried out on regenerated nerve fibers. In vitro investigation showed the formation of typical neuroglial cells after differentiation, which were positively stained for the typical specific neuroglial markers such as the GFAP, the GAP-43 and NeuN. NMR showed clear evidence that HMSCs expansion is glycolysis-dependent but their differentiation requires the switch of the metabolic profile to oxidative metabolism. In vivo studies showed enhanced recovery of motor and sensory function in animals treated with transplanted undifferentiated and differentiated HMSCs that was accompanied by an increase in myelin sheath. Taken together, HMSC from the umbilical cord Wharton jelly might be useful for improving the clinical outcome after peripheral nerve lesion.
authors.affiliation: Institute of Biomedical Sciences Abel Salazar, Porto University, Portugal.
authors.forename: A
authors.initials: A
authors.lastname: Gärtner
authors.forename: T
authors.initials: T
authors.lastname: Pereira
authors.forename: Marco G
authors.initials: MG
authors.lastname: Alves
authors.forename: P A S
authors.initials: PA
authors.lastname: Armada-da-Silva
authors.forename: I
authors.initials: I
authors.lastname: Amorim
authors.forename: R
authors.initials: R
authors.lastname: Gomes
authors.forename: J
authors.initials: J
authors.lastname: Ribeiro
authors.forename: M L
authors.initials: ML
authors.lastname: França
authors.forename: C
authors.initials: C
authors.lastname: Lopes
authors.forename: Rui A
authors.initials: RA
authors.lastname: Carvalho
authors.forename: S
authors.initials: S
authors.lastname: Socorro
authors.forename: Pedro F
authors.initials: PF
authors.lastname: Oliveira
authors.forename: B
authors.initials: B
authors.lastname: Porto
authors.forename: R
authors.initials: R
authors.lastname: Sousa
authors.forename: A
authors.initials: A
authors.lastname: Bombaci
authors.forename: G
authors.initials: G
authors.lastname: Ronchi
authors.forename: F
authors.initials: F
authors.lastname: Fregnan
authors.forename: A S P
authors.initials: AS
authors.lastname: Varejão
authors.forename: A L
authors.initials: AL
authors.lastname: Luís
authors.forename: S
authors.initials: S
authors.lastname: Geuna
authors.forename: A C
authors.initials: AC
authors.lastname: Maurício
mesh_terms: D000818:Animals; D034961:Antigens, Nuclear / Q000032:analysis; D002454:Cell Differentiation; D002460:Cell Line; D019922:GAP-43 Protein / Q000032:analysis; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006019:Glycolysis; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008567:Membranes, Artificial; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D009043:Motor Activity; D009186:Myelin Sheath / Q000378:metabolism; D009409:Nerve Crush; D009416:Nerve Regeneration; D009419:Nerve Tissue Proteins / Q000032:analysis; D009457:Neuroglia / Q000166:cytology; D059348:Peripheral Nerve Injuries / Q000628:therapy; D011091:Polyesters / Q000627:therapeutic use*; D051381:Rats; D012584:Sciatic Nerve / Q000737:chemistry / Q000502:physiology / Q000601:surgery; D012677:Sensation; D059631:Wharton Jelly / Q000166:cytology
chemical_list: D034961:Antigens, Nuclear; D019922:GAP-43 Protein; D005904:Glial Fibrillary Acidic Protein; D008567:Membranes, Artificial; D009419:Nerve Tissue Proteins; D011091:Polyesters; C546339:Rbfox3 protein, rat; C062968:lactide-caprolactone copolymer