title: Ionic matrices pre-spotted matrix-assisted laser desorption/ionization plates for patient maker following in course of treatment, drug titration, and MALDI mass spectrometry imaging.
abstract: In the current study, we compared plastic matrix-assisted laser desorption/ionization (MALDI) plates pre-spotted with different solid ionic matrices. Data reflect that after 3 months of storage, the standards were oxidized in α-cyano-4-hydroxycinnamic acid (HCCA) whether or not in HCCA/3-acetylpyridine (3APY) and HCCA/aniline, and certain peptides, such as ubiquitin, were not detected using the HCCA matrix, whereas they were detected in pre-spotted ionic matrices. Application in peptidomics of these MALDI matrices pre-spotted plates (after 3 months of storage) with ovarian cyst fluid showed less intense signals with HCCA than with solid ionic matrices. We show that these pre-spotted ionic matrices plates can be used for relative drug quantification, high mass protein detection, and MALDI mass spectrometry imaging.
authors.affiliation: Laboratoire de Spectrométrie de Masse Biologique Fondamentale et Appliquée (FABMS), Université Lille 1, 59655 Villeneuve d'Ascq, France.
authors.forename: David
authors.initials: D
authors.lastname: Bonnel
authors.forename: Julien
authors.initials: J
authors.lastname: Franck
authors.forename: Céline
authors.initials: C
authors.lastname: Mériaux
authors.forename: Michel
authors.initials: M
authors.lastname: Salzet
authors.forename: Isabelle
authors.initials: I
authors.lastname: Fournier
mesh_terms: D058671:Adrenergic beta-1 Receptor Antagonists / Q000032:analysis; D000814:Aniline Compounds / Q000737:chemistry; D000818:Animals; D001262:Atenolol / Q000032:analysis; D001921:Brain / Q000378:metabolism; D003373:Coumaric Acids / Q000737:chemistry; D020420:Cyst Fluid / Q000378:metabolism; D005260:Female; D006801:Humans; D007477:Ions / Q000737:chemistry; D008297:Male; D010455:Peptides / Q000032:analysis; D004364:Pharmaceutical Preparations / Q000032:analysis*; D010655:Phenylenediamines / Q000737:chemistry; D011725:Pyridines / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000592:standards; D025801:Ubiquitin / Q000032:analysis
chemical_list: D058671:Adrenergic beta-1 Receptor Antagonists; D000814:Aniline Compounds; D003373:Coumaric Acids; D007477:Ions; D010455:Peptides; D004364:Pharmaceutical Preparations; D010655:Phenylenediamines; D011725:Pyridines; D025801:Ubiquitin; C010835:3-acetylpyridine; C007175:alpha-cyano-4-hydroxycinnamate; D001262:Atenolol; C023650:aniline