title: A novel cyclic RGD-containing peptide polymer improves serum-free adhesion of adipose tissue-derived mesenchymal stem cells to bone implant surfaces.
abstract: Seeding of bone implants with mesenchymal stem cells (MSCs) may promote osseointegration and bone regeneration. However, implant material surfaces, such as titanium or bovine bone mineral, fail to support rapid and efficient attachment of MSCs, especially under serum-free conditions that may be desirable when human applications or tightly controlled experiments are envisioned. Here we demonstrate that a branched poly[Lys(Ser(i)-DL-Ala(m))] polymer functionalized with cyclic arginyl-glycyl-aspartate, when immobilized by simple adsorption to tissue culture plastic, surgical titanium alloy (Ti6Al4V), or Bio-Oss(®) bovine bone substitute, significantly accelerates serum-free adhesion and enhances seeding efficiency of human adipose tissue-derived MSCs. Moreover, when exposed to serum-containing osteogenic medium, MSCs survived and differentiated on the peptide-coated scaffolds. In summary, the presented novel polypeptide conjugate can be conveniently used for coating various surfaces, and may find applications whenever quick and efficient seeding of MSCs is required to various scaffolds in the absence of serum.
authors.affiliation: Research Center for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Tátrai
authors.forename: Bernadett
authors.initials: B
authors.lastname: Sági
authors.forename: Anna
authors.initials: A
authors.lastname: Szigeti
authors.forename: Aron
authors.initials: A
authors.lastname: Szepesi
authors.forename: Ildikó
authors.initials: I
authors.lastname: Szabó
authors.forename: Szilvia
authors.initials: S
authors.lastname: Bősze
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Kristóf
authors.forename: Károly
authors.initials: K
authors.lastname: Markó
authors.forename: Gergely
authors.initials: G
authors.lastname: Szakács
authors.forename: István
authors.initials: I
authors.lastname: Urbán
authors.forename: Gábor
authors.initials: G
authors.lastname: Mező
authors.forename: Ferenc
authors.initials: F
authors.lastname: Uher
authors.forename: Katalin
authors.initials: K
authors.lastname: Német
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects; D000328:Adult; D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D016025:Bone Transplantation / Q000379:methods; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D008954:Models, Biological; D016348:Osseointegration / Q000187:drug effects / Q000502:physiology; D010456:Peptides, Cyclic / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology; D013499:Surface Properties / Q000187:drug effects
chemical_list: D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible; D016895:Culture Media, Serum-Free; D010456:Peptides, Cyclic; D011108:Polymers; C507169:cyclic arginine-glycine-aspartic acid peptide