title: Characterization of the fungal microflora in raw milk and specialty cheeses of the province of Quebec.
abstract: The cheese microbial ecosystem is complex, and the presence of non-starter adventitious microorganisms in milk may have an influence on the organoleptic characteristics of cheese. The aim of this study was to analyze the composition and diversity of the fungal flora of raw milk destined for cheesemaking from 19 dairy farms in Quebec and to monitor their evolution throughout ripening. Six hundred ten yeast and mold isolates were collected from raw milk and raw milk cheeses over a 9-month period. Based on the sequences of the rDNA ITS1-5.8S-ITS2 region, 67% of the raw milk isolates were yeasts, which were assigned to 37 species across 11 genera, while 33% were molds, which were assigned to 33 species across 25 genera. A semi-quantitative analysis of the yeasts and molds in the raw milk from four farms was performed over a 5-month period. The composition and diversity of the fungal microflora were totally different for each farm, each of which had a unique species profile. To determine whether adventitious yeast strains from the milk could develop in raw milk cheese, a multilocus-sequence-typing (MLST) analysis was performed on 13 Issatchenkia orientalis (syn. Pichia kudriavzevii, anamorph: Candida krusei) isolates. The same MLST genotypes were identified for strains independently isolated from raw milk and raw milk cheese from a farm processing its own milk. This study contributes to the understanding of the natural fungal microflora of raw milk and suggests that non-starter yeasts and molds can transfer from raw milk to raw milk cheese and may influence cheese ripening. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13594-011-0051-4) contains supplementary material, which is available to authorized users.
authors.affiliation: Nutraceuticals and Functional Foods Institute (INAF) and STELA Dairy Research Centre, Québec, QC Canada  ; Department of Food Sciences and Nutrition, Université Laval, 2425 rue de l'Agriculture, Pavillon Paul-Comtois, Québec, QC Canada G1V 0A6.
authors.forename: Karine
authors.initials: K
authors.lastname: Lavoie
authors.forename: Marilyne
authors.initials: M
authors.lastname: Touchette
authors.forename: Daniel
authors.initials: D
authors.lastname: St-Gelais
authors.forename: Steve
authors.initials: S
authors.lastname: Labrie