title: Expression of corticosteroid binding globulin in the rat olfactory system.
abstract: Glucocorticoids are known to act on the olfactory system although their mode of action is still unclear since nuclear glucocorticoid receptors are mostly absent in the olfactory mucosa. In this study we used immunocytochemistry, in situ hybridization, and RT-PCR to study the expression and distribution of corticosteroid binding globulin (CBG) in the rat olfactory system. Mucosal goblet cells could be immunostained for CBG. Nasal secretion contained measurable amounts of CBG suggesting that CBG is liberated. CBG immunoreactivity was localized in many of the basal cells of the olfactory mucosa, while mature sensory cells contained CBG only in processes as determined by double immunostaining with the olfactory marker protein OMP. This staining was most pronounced in the vomeronasal organ (VNO). The appearance of CBG in the non-sensory and sensory parts of the VNO and in nerve terminals in the accessory bulb indicated axonal transport. Portions of the periglomerular cells, the mitral cells and the tufted cells were also CBG positive. CBG encoding transcripts were confirmed by RT-PCR in homogenates of the olfactory mucosa and VNO. Olfactory CBG may be significant for uptake, accumulation and transport of glucocorticoids, including aerosolic cortisol.
authors.affiliation: Department of Anatomy II, Friedrich Schiller Universität Jena, Germany.
authors.forename: Wilfried
authors.initials: W
authors.lastname: Dölz
authors.forename: Annett
authors.initials: A
authors.lastname: Eitner
authors.forename: Jack D
authors.initials: JD
authors.lastname: Caldwell
authors.forename: Gustav F
authors.initials: GF
authors.lastname: Jirikowski
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D009831:Olfactory Mucosa / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D051381:Rats; D014156:Transcortin / Q000235:genetics* / Q000378:metabolism*
chemical_list: D014156:Transcortin