title: StemCellDB: the human pluripotent stem cell database at the National Institutes of Health.
abstract: Much of the excitement generated by induced pluripotent stem cell technology is concerned with the possibility of disease modeling as well as the potential for personalized cell therapy. However, to pursue this it is important to understand the 'normal' pluripotent state including its inherent variability. We have performed various molecular profiling assays for 21 hESC lines and 8 hiPSC lines to generate a comprehensive snapshot of the undifferentiated state of pluripotent stem cells. Analysis of the gene expression data revealed no iPSC-specific gene expression pattern in accordance with previous reports. We further compared cells, differentiated as embryoid bodies in 2 media proposed to initiate differentiation towards separate cell fates, as well as 20 adult tissues. From this analysis we have generated a gene list which defines pluripotency and establishes a baseline for the pluripotent state. Finally, we provide lists of genes enriched under both differentiation conditions which show the proposed bias toward independent cell fates.
authors.affiliation: NIH Stem Cell Unit, Division of Intramural Research, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA. mallonb@mail.nih.gov
authors.forename: Barbara S
authors.initials: BS
authors.lastname: Mallon
authors.forename: Josh G
authors.initials: JG
authors.lastname: Chenoweth
authors.forename: Kory R
authors.initials: KR
authors.lastname: Johnson
authors.forename: Rebecca S
authors.initials: RS
authors.lastname: Hamilton
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Tesar
authors.forename: Amarendra S
authors.initials: AS
authors.lastname: Yavatkar
authors.forename: Leonard J
authors.initials: LJ
authors.lastname: Tyson
authors.forename: Kyeyoon
authors.initials: K
authors.lastname: Park
authors.forename: Kevin G
authors.initials: KG
authors.lastname: Chen
authors.forename: Yang C
authors.initials: YC
authors.lastname: Fann
authors.forename: Ronald D G
authors.initials: RD
authors.lastname: McKay
mesh_terms: D000818:Animals; D002460:Cell Line; D016208:Databases, Factual; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice; D009316:National Institutes of Health (U.S.); D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D025341:Principal Component Analysis; D014481:United States