title: Subcellular and subnuclear distribution of high-light responsive serine/arginine-rich proteins, atSR45a and atSR30, in Arabidopsis thaliana.
abstract: Here, we demonstrated the involvement of the domains in Arabidopsis high-light responsive serine/arginine-rich (SR) and SR-like proteins, atSR30 and atSR45a, respectively, in subcellular and subnuclear distribution using a series of structural domain-deleted mutants. Judging from the localization of the transiently expressed domain-deleted mutants in onion epidermal cells, the C terminal low complexity domain rich in arginine-serine repeats (C-RS) domain of atSR30 appeared to be necessary for the nuclear localization. On the other hand, the N-terminal RS (N-RS) domain of atSR45a was necessary for the accurate nuclear localization, although the N- or C-RS domain alone was sufficient for the nuclear speckled organization. The phosphorylation of RS domains of atSR45a is irrelevant to the regulation of its localization. atSR45a and atSR30 were co-localized in the speckles, suggesting their collaborative roles in the regulation of alternative splicing events.
authors.affiliation: Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, 3327-204 Nakamachi, Nara 631-8505, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Mori
authors.forename: Kazuya
authors.initials: K
authors.lastname: Yoshimura
authors.forename: Ryota
authors.initials: R
authors.lastname: Nosaka
authors.forename: Harumi
authors.initials: H
authors.lastname: Sakuyama
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Koike
authors.forename: Noriaki
authors.initials: N
authors.lastname: Tanabe
authors.forename: Takanori
authors.initials: T
authors.lastname: Maruta
authors.forename: Masahiro
authors.initials: M
authors.lastname: Tamoi
authors.forename: Shigeru
authors.initials: S
authors.lastname: Shigeoka
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000528:radiation effects; D017398:Alternative Splicing / Q000528:radiation effects; D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001120:Arginine; D002467:Cell Nucleus / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism*; D008027:Light; D019697:Onions / Q000166:cytology; D010766:Phosphorylation / Q000528:radiation effects; D017434:Protein Structure, Tertiary; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017384:Sequence Deletion; D012694:Serine; D000068103:Serine-Arginine Splicing Factors
chemical_list: D029681:Arabidopsis Proteins; D016601:RNA-Binding Proteins; C581090:SR30 protein, Arabidopsis; C569740:SR45a protein, Arabidopsis; D000068103:Serine-Arginine Splicing Factors; D012694:Serine; D001120:Arginine