title: Multiplex PCR assay targeting a diguanylate cyclase-encoding gene, cgcA, to differentiate species within the genus Cronobacter.
abstract: In a comparison to the widely used Cronobacter rpoB PCR assay, a highly specific multiplexed PCR assay based on cgcA, a diguanylate cyclase gene, that identified all of the targeted six species among 305 Cronobacter isolates was designed. This assay will be a valuable tool for identifying suspected Cronobacter isolates from food-borne investigations.
authors.affiliation: CFSAN, FDA, Laurel, Maryland, USA.
authors.forename: L
authors.initials: L
authors.lastname: Carter
authors.forename: L A
authors.initials: LA
authors.lastname: Lindsey
authors.forename: C J
authors.initials: CJ
authors.lastname: Grim
authors.forename: V
authors.initials: V
authors.lastname: Sathyamoorthy
authors.forename: K G
authors.initials: KG
authors.lastname: Jarvis
authors.forename: G
authors.initials: G
authors.lastname: Gopinath
authors.forename: C
authors.initials: C
authors.lastname: Lee
authors.forename: J A
authors.initials: JA
authors.lastname: Sadowski
authors.forename: L
authors.initials: L
authors.lastname: Trach
authors.forename: M
authors.initials: M
authors.lastname: Pava-Ripoll
authors.forename: B A
authors.initials: BA
authors.lastname: McCardell
authors.forename: B D
authors.initials: BD
authors.lastname: Tall
authors.forename: L
authors.initials: L
authors.lastname: Hu
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D059126:Cronobacter / Q000145:classification* / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D029968:Escherichia coli Proteins / Q000235:genetics*; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000175:diagnosis / Q000382:microbiology; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D019761:Phosphorus-Oxygen Lyases / Q000235:genetics*; D012680:Sensitivity and Specificity
chemical_list: D029968:Escherichia coli Proteins; D019761:Phosphorus-Oxygen Lyases; C060720:diguanylate cyclase