title: Fast human serum profiling through chemical depletion coupled to gold-nanoparticle-assisted protein separation.
abstract: The use of chemical protein depletion in conjunction with gold-based nanoparticles for fast matrix assisted laser desoption ionization time of flight mass spectrometry-based human serum profiling was assessed. The following variables influencing the process were optimized: (i) amount of nanoparticles, (ii) sample pH, (iii) amount of protein and (iv) incubation time. pH was found the most important factor to be controlled, with an optimum range comprised between 5.8 and 6.4. The minimum incubation time to obtain an adequate profiling was 30 min. Using this approach, serum from five patients with lymphoma, five patients with myeloma and from two healthy volunteers were correctly classified using Principal component analysis.
authors.affiliation: Departamento de Química-Física, Facultad de Ciencias, Campus de Ourense, Universidad de Vigo, 32004 Ourense, Spain.
authors.forename: Rubén
authors.initials: R
authors.lastname: López-Cortés
authors.forename: Elisabete
authors.initials: E
authors.lastname: Oliveira
authors.forename: Cristina
authors.initials: C
authors.lastname: Núñez
authors.forename: Carlos
authors.initials: C
authors.lastname: Lodeiro
authors.forename: María
authors.initials: M
authors.lastname: Páez de la Cadena
authors.forename: Florentino
authors.initials: F
authors.lastname: Fdez-Riverola
authors.forename: H
authors.initials: H
authors.lastname: López-Fernández
authors.forename: Miguel
authors.initials: M
authors.lastname: Reboiro-Jato
authors.forename: Daniel
authors.initials: D
authors.lastname: Glez-Peña
authors.forename: José
authors.initials: J
authors.lastname: Luis Capelo
authors.forename: Hugo M
authors.initials: HM
authors.lastname: Santos
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods*; D001798:Blood Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D002417:Cattle; D005591:Chemical Fractionation / Q000379:methods*; D005260:Female; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008223:Lymphoma / Q000097:blood; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D008875:Middle Aged; D009101:Multiple Myeloma / Q000097:blood; D013997:Time Factors
chemical_list: D001798:Blood Proteins; D006046:Gold