title: Simultaneous separation and purification of total polyphenols, chlorogenic acid and phlorizin from thinned young apples.
abstract: An efficient preparative separation of polyphenols from thinned young apples (TYA) has been developed in the present study. X-5 resin was verified to offer the best adsorption capacity and desorption ratio for total polyphenols among the eight macroporous resins investigated. Influential factors, such as pH value and concentration of feeding solution, strippant, and adsorption isotherm to the separation of total polyphenols, were successively investigated on X-5 resin. After one run treatment, the phenolic content was increased 2.12-fold from 35.17% to 74.64%, with a recovery yield of 89.35%. Chlorogenic acid and phlorizin were selectively purified using X-5 and polyamide resins. The contents of chlorogenic acid and phlorizin were 15.20% and 97.52% with recovery yields of 89.16% and 64.95%, respectively. The method developed will provide a potential approach for its wide industrial and pharmaceutical use.
authors.affiliation: College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710062, PR China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Sun
authors.forename: Yurong
authors.initials: Y
authors.lastname: Guo
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Fu
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Li
mesh_terms: D000327:Adsorption; D002726:Chlorogenic Acid / Q000737:chemistry / Q000302:isolation & purification*; D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D027845:Malus / Q000737:chemistry* / Q000254:growth & development; D010695:Phlorhizin / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D059808:Polyphenols / Q000737:chemistry / Q000302:isolation & purification*; D012117:Resins, Synthetic / Q000737:chemistry
chemical_list: D010936:Plant Extracts; D059808:Polyphenols; D012117:Resins, Synthetic; D002726:Chlorogenic Acid; D010695:Phlorhizin