title: Iron oxide particles modulate the ovalbumin-induced Th2 immune response in mice.
abstract: This study was designed to investigate the modulatory effects of submicron and nanosized iron oxide (Fe(2)O(3)) particles on the ovalbumin (OVA)-induced immune Th2 response in BALB/c mice. Particles were intratracheally administered four times to mice before and during the OVA sensitization period. For each particle type, three different doses, namely 4×100, 4×250 or 4×500 μg/mouse, were used and for each dose, four groups of mice, i.e. group saline solution (1), OVA (2), particles (3), and OVA plus particles (4), were constituted. Mice exposed to OVA alone exhibited an allergic Th2-dominated response with a consistent increase in inflammatory scores, eosinophil numbers, specific IgE levels and IL-4 production. When the mice were exposed to OVA and to high and intermediate doses of iron oxide submicron- or nanoparticles, the OVA-induced allergic response was significantly inhibited, as evidenced by the decrease in eosinophil cell influx and specific IgE levels. However, the low dose (4×100 μg) of submicron particles had no significant effect on the OVA allergic response while the same dose of nanoparticles had an adjuvant effect on the Th2 response to OVA. In conclusion, these data demonstrate that the pulmonary immune response to OVA is a sensitive target for intratracheally instilled particles. Depending on the particle dose and size, the allergic response was suppressed or enhanced.
authors.affiliation: Department of Pollutants and Health, National Institute for Research and Safety 54519 Vandoeuvre-les-Nancy, France.
authors.forename: Masarin
authors.initials: M
authors.lastname: Ban
authors.forename: Isabelle
authors.initials: I
authors.lastname: Langonné
authors.forename: Nelly
authors.initials: N
authors.lastname: Huguet
authors.forename: Yves
authors.initials: Y
authors.lastname: Guichard
authors.forename: Michèle
authors.initials: M
authors.lastname: Goutet
mesh_terms: D056704:Adaptive Immunity / Q000187:drug effects*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004342:Drug Hypersensitivity / Q000188:drug therapy*; D005260:Female; D005290:Ferric Compounds / Q000737:chemistry / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000502:physiology; D007073:Immunoglobulin E / Q000097:blood; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008171:Lung Diseases / Q000139:chemically induced* / Q000188:drug therapy / Q000276:immunology; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000633:toxicity*
chemical_list: D016207:Cytokines; D005290:Ferric Compounds; C000499:ferric oxide; D007073:Immunoglobulin E; D010047:Ovalbumin