title: Do not put all teleosts in one net: focus on the sox2 and pou2 genes.
abstract: The Pou2 and Sox2 proteins are major transcription factors for development and cell differentiation. In teleosts, the expression patterns of pou2 or sox2 are different between species from distant families, suggesting different regulatory mechanisms of gene expression. In this study, we assessed the divergences among teleosts, including within closely related species. The pou2 and sox2 gene expression patterns were characterised over several developmental stages in a cyprinid model, i.e., the goldfish, and the potential regulation sites of these genes within teleost conserved regions were localised. During embryonic development, differences in the expression patterns between the goldfish and other teleosts, including zebrafish, were observed for both genes. The in silico analysis of the 5' flanking regions of the pou2 gene showed high conservation within teleosts, whereas the sox2 sequence diverged in tetraodontiforms. Certain putative cis regulatory elements were common to all teleosts, whereas others were found only in cyprinids. The analysis of the DNA methylation patterns of the pou2 and sox2 upstream sequences revealed that the studied CpG sites remained hypomethylated at all stages of embryo development in both genes. In contrast, in the adult fin, the studied CpG sites were hypermethylated in pou2 but not in sox2, suggesting the existence of methylation-sensitive regions in pou2. Overall, although most similarities at the level of the gene regulatory sites were found within cyprinids, the expression pattern of pou2 or sox2 during development differs between cyprinids species.
authors.affiliation: INRA, UR1037 Fish Physiology and Genomics, Biogenouest, Campus de Beaulieu, F-35000 Rennes, France.
authors.forename: Lucie
authors.initials: L
authors.lastname: Marandel
authors.forename: Catherine
authors.initials: C
authors.lastname: Labbe
authors.forename: Julien
authors.initials: J
authors.lastname: Bobe
authors.forename: Hélène
authors.initials: H
authors.lastname: Jammes
authors.forename: Jean-Jaques
authors.initials: JJ
authors.lastname: Lareyre
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Le Bail
mesh_terms: D024506:5' Flanking Region / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003530:Cyprinidae / Q000145:classification / Q000235:genetics; D019175:DNA Methylation; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000196:embryology / Q000378:metabolism; D005260:Female; D029941:Fish Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D006054:Goldfish / Q000196:embryology / Q000235:genetics*; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D050814:Octamer Transcription Factor-3 / Q000235:genetics*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055748:SOXB1 Transcription Factors / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D024363:Transcription Initiation Site
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D050814:Octamer Transcription Factor-3; D055748:SOXB1 Transcription Factors