title: Evaluation of the enhancing ability of three adjuvants for DNA vaccination using the porcine circovirus type 2 ORF2 (capsid) gene in mice.
abstract: Molecular adjuvants were used to augment the amplitude of the immune response in many studies recently. Ubiquitin (ub), the peptide binding truncated C-terminal portion of heat shock protein 70 (hsp70c) and interleukin-2 (IL-2) are widely investigated adjuvants which have been proved to be efficient. In our study, we compared the enhancing ability of these three adjuvants based on DNA vaccination using the porcine circovirus type 2 ORF2 (capsid) gene in mice. The results of lymphocyte proliferation assay, flow cytometric analysis (FCM), antibody titer and cytokine production showed that ub conjugated plasmid induced a stronger Th1 type cellular immune response and an observably higher level of Cap-specific serum immunoglobulin G antibody compared with hsp70c or IL-2 conjugated plasmids during the period of post-immunization. Meanwhile, the ub conjugation vaccinated group elicited stronger specific immunity against PCV2 challenge than the others during most of the time of post-challenge. Thus, these data indicate that ub is a superior adjuvant for a PCV2 DNA vaccination than the hsp70c and IL-2 molecules.
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 427, Maduan Street, Nangang District, Harbin 150001, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Fu
authors.forename: Yuekun
authors.initials: Y
authors.lastname: Lang
authors.forename: Xuesong
authors.initials: X
authors.lastname: Li
authors.forename: Zheng
authors.initials: Z
authors.lastname: Chai
authors.forename: Guangzhi
authors.initials: G
authors.lastname: Tong
authors.forename: Siguo
authors.initials: S
authors.lastname: Liu
authors.forename: Huabin
authors.initials: H
authors.lastname: Tian
authors.forename: Haizhong
authors.initials: H
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Xi
authors.initials: X
authors.lastname: Li
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D036022:Capsid Proteins / Q000235:genetics* / Q000276:immunology; D018173:Circoviridae Infections / Q000662:veterinary; D017925:Circovirus / Q000235:genetics* / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D005260:Female; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D051379:Mice; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control / Q000821:virology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D019444:Vaccines, DNA / Q000235:genetics* / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D036022:Capsid Proteins; D016207:Cytokines; D007074:Immunoglobulin G; D019444:Vaccines, DNA