title: Analysis of volatile compounds of Antrodia camphorata in submerged culture using headspace solid-phase microextraction.
abstract: In this work a headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS) and GC-olfactometry (GC-O) was developed to evaluate the profile of the volatile compounds that contribute to the aroma of Antrodia camphorata in submerged culture. For this purpose, the HS-SPME sampling method for the volatile compounds of A. camphorata in submerged culture was optimised by a D-optimal design. A HS extraction of the culture broth of A. camphorata followed by incubation on a carboxen/polydimethylsiloxane (CAR/PDMS) fibre during 31.8min at 54.6°C gave the most effective and accurate extraction of the volatile compounds. By the optimised method, a total of 49 volatile compounds were identified in culture broth of A. camphorata, while a total of 55 volatile compounds were identified in the mycelia. A series of C(8) aliphatic compounds (mushroom-like odour), several lactones (fruity odour) and l-linalool (citrus-like odour) were the most potent key odourant in both the mycelia and culture broth. This combined technique is fast, simple, sensitive, inexpensive and useful to monitor volatile compounds associated to A. camphorata.
authors.affiliation: Laboratory of Pharmaceutical Engineering, School of Medicine and Pharmaceutics, Jiangnan University, Wuxi 214122, PR China; The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Zhen-Ming
authors.initials: ZM
authors.lastname: Lu
authors.forename: Wen-Yi
authors.initials: WY
authors.lastname: Tao
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Xu
authors.forename: Joanne
authors.initials: J
authors.lastname: Lim
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Zhang
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Wang
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Chen
authors.forename: Zheng-Hong
authors.initials: ZH
authors.lastname: Xu