title: Use of viscera extract from hybrid catfish (Clarias macrocephalus × Clarias gariepinus) for the production of protein hydrolysate from toothed ponyfish (Gazza minuta) muscle.
abstract: Proteolytic activity of viscera extract from hybrid catfish (Clarias macrocephalus × Clarias gariepinus) was studied. The optimal pH and temperature were 9.0 and 50°C, respectively, when toothed ponyfish (Gazza minuta) muscle was used as a substrate. When viscera extract from hybrid catfish was used for the production of protein hydrolysate from toothed ponyfish muscle, extract concentration, reaction time, and fish muscle/buffer ratio affected the hydrolysis and nitrogen recovery (NR) (p<0.05). Optimum conditions for toothed ponyfish muscle hydrolysis were 3.5% hybrid catfish viscera extract, 15 min reaction time and fish muscle/buffer ratio of 1:3 (w/v). High correlation between the degree of hydrolysis (DH) and NR (R(2)=0.974) was observed. Freeze-dried hydrolysate had a high protein content (89.02%, dry weight basis) and it was brownish yellow in colour (L(∗)=63.67, a(∗)=6.33, b(∗)=22.41). The protein hydrolysate contained a high amount of essential amino acids (48.22%) and had arginine and lysine as the dominant amino acids.
authors.affiliation: Department of Food Science and Technology, Faculty of Technology and Community Development, Thaksin University, Phatthalung Campus, Phatthalung 93110, Thailand. sappasith@tsu.ac.th
authors.forename: Sappasith
authors.initials: S
authors.lastname: Klomklao
authors.forename: Hideki
authors.initials: H
authors.lastname: Kishimura
authors.forename: Soottawat
authors.initials: S
authors.lastname: Benjakul
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002397:Catfishes / Q000235:genetics*; D004338:Drug Combinations; D004795:Enzyme Stability; D029941:Fish Proteins / Q000737:chemistry*; D006824:Hybridization, Genetic; D006868:Hydrolysis; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry*; D010206:Papain / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000737:chemistry*; D010473:Perciformes; D011492:Protein Hydrolysates / Q000737:chemistry*; D017747:Seafood / Q000032:analysis; D012982:Sodium, Dietary; D014781:Viscera / Q000737:chemistry / Q000201:enzymology*
chemical_list: D000596:Amino Acids; D004338:Drug Combinations; D029941:Fish Proteins; D011492:Protein Hydrolysates; D012982:Sodium, Dietary; C032508:meat tenderizer; D010447:Peptide Hydrolases; D010206:Papain