title: Comparative analysis of peroxidase profiles in Chinese kale (Brassica alboglabra L.): evaluation of leaf growth related isozymes.
abstract: Plant peroxidases (EC 1.11.1.7) with different isoforms catalyze various reactions in plant growth and development. However, it is difficult to elucidate the function of each isozyme in one plant. Here, we compared profiles of entire isozyme in young seedling and mature leaves of Chinese kale (Brassica alboglabra L.) on zymogram and ion exchange chromatography in order to investigate leaf growth related peroxidase isozymes. The results showed that four isozymes were constitutively expressed in kale leaves, whereas other two isozymes were induced in the mature leaves. The Mono Q ion exchange chromatography separated the six isozymes into two major groups due to the difference in their isoelectric points. The results suggested that although there were several isozymes in the leaves of Chinese kale, one isozyme functioned mainly through the leaf development. Two anionic isozymes with molecular weights lower than 32 kDa were considered mature related.
authors.affiliation: Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, No. 1800 Lihu Avenue, Wuxi, Jiangsu 214122, China. ijtang@hotmail.com
authors.forename: Lei
authors.initials: L
authors.lastname: Tang
authors.forename: Chenchen
authors.initials: C
authors.lastname: Wang
authors.forename: Jiabao
authors.initials: J
authors.lastname: Huang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhonggui
authors.initials: Z
authors.lastname: Mao
authors.forename: Haiou
authors.initials: H
authors.lastname: Wang
mesh_terms: D001937:Brassica / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D007526:Isoelectric Point; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008970:Molecular Weight; D009195:Peroxidase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D010940:Plant Proteins; D009195:Peroxidase