title: Structure of Escherichia coli RutC, a member of the YjgF family and putative aminoacrylate peracid reductase of the rut operon.
abstract: RutC is the third enzyme in the Escherichia coli rut pathway of uracil degradation. RutC belongs to the highly conserved YjgF family of proteins. The structure of the RutC protein was determined and refined to 1.95 Å resolution. The crystal belonged to space group P2(1)2(1)2 and contained six molecules in the asymmetric unit. The structure was solved by SAD phasing and was refined to an Rwork of 19.3% (Rfree=21.7%). The final model revealed that this protein has a Bacillus chorismate mutase-like fold and forms a homotrimer with a hydrophobic cavity in the center of the structure and ligand-binding clefts between two subunits. A likely function for RutC is the reduction of peroxy-aminoacrylate to aminoacrylate as a part of a detoxification process.
authors.affiliation: Department of Molecular Physiology and Biological Physics, University of Virginia, 1340 Jefferson Park Avenue, Jordan Hall, Charlottesville, VA 22908, USA.
authors.forename: Aleksandra Alicja
authors.initials: AA
authors.lastname: Knapik
authors.forename: Janusz Jurand
authors.initials: JJ
authors.lastname: Petkowski
authors.forename: Zbyszek
authors.initials: Z
authors.lastname: Otwinowski
authors.forename: Marcin Tadeusz
authors.initials: MT
authors.lastname: Cymborowski
authors.forename: David Robert
authors.initials: DR
authors.lastname: Cooper
authors.forename: Maksymilian
authors.initials: M
authors.lastname: Chruszcz
authors.forename: Wanda Małgorzata
authors.initials: WM
authors.lastname: Krajewska
authors.forename: Wladek
authors.initials: W
authors.lastname: Minor
mesh_terms: D000595:Amino Acid Sequence; D020134:Catalytic Domain; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000737:chemistry*; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D009876:Operon; D010088:Oxidoreductases / Q000737:chemistry*; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D040681:Structural Homology, Protein
chemical_list: D029968:Escherichia coli Proteins; D010088:Oxidoreductases; C580879:RutC protein, E coli