title: [Determination of plasma protein binding rate of vitexin].
abstract: OBJECTIVE
  To establish an analytical method on vitexin concentration in plasma to determine the plasma protein binding rate of vitexin.
  
  
  METHODS
  The equilibrium dialysis method and HPLC were adopted to determine vitexin concentration in plasma, calculate human plasma protein binding rate of vitexin and compare rat and human plasma protein binding rates of vitexin.
  
  
  RESULTS
  At 2-16 mg x L(-1), there was no significant difference in the plasma protein binding rate. But the human plasma protein binding rate of vitexin was higher than its rat plasma protein binding rate, indicating a significant difference in rat and human plasma protein binding rates of vitexin.
  
  
  CONCLUSIONS
  Vitexin has a higher protein binding rate with both rat plasma and human plasma.
authors.affiliation: Anhui Institute for Food and Drug Control, Hefei 230051, China. tclls@163.com
authors.forename: Chengliang
authors.initials: C
authors.lastname: Tong
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wu
mesh_terms: D000818:Animals; D047310:Apigenin / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003956:Dialysis; D006801:Humans; D011485:Protein Binding; D051381:Rats; D013045:Species Specificity
chemical_list: D001798:Blood Proteins; D047310:Apigenin; C032731:vitexin