title: Quantitative real-time PCR assay for detection of Paenibacillus polymyxa using membrane-fusion protein-based primers.
abstract: Paenibacillus polymyxa is known to be a plant-growthpromoting rhizobacterium. The present study describes a quantitative polymerase chain reaction (qPCR) assay for the specific detection and quantitation of P. polymyxa using a primer pair based on the sequence of a membranefusion protein for the amplification of a 268 bp DNA fragment. This study reports that the qPCR-based method is applicable for the rapid and sensitive detection of P. polymyxa and can be used as an alternative method for agricultural soil monitoring.
authors.affiliation: Smarteome Co., Ltd., Suwon 441-853, Korea. minseok.cho.kr@gmail.com
authors.forename: Min Seok
authors.initials: MS
authors.lastname: Cho
authors.forename: Dong Suk
authors.initials: DS
authors.lastname: Park
authors.forename: Jung Won
authors.initials: JW
authors.lastname: Lee
authors.forename: Hee Youn
authors.initials: HY
authors.lastname: Chi
authors.forename: Soo-In
authors.initials: SI
authors.lastname: Sohn
authors.forename: Bong-Kyun
authors.initials: BK
authors.lastname: Jeon
authors.forename: Jong-Beom
authors.initials: JB
authors.lastname: Ma
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics*; D050576:Membrane Fusion Proteins / Q000235:genetics* / Q000378:metabolism; D056507:Paenibacillus / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D050576:Membrane Fusion Proteins; D011993:Recombinant Fusion Proteins