title: Aeromonas cavernicola sp. nov., isolated from fresh water of a brook in a cavern.
abstract: Aeromonas P2973 was isolated from the water of a brook in a cavern in the Czech Republic. This isolate could not be biochemically identified at the species level, considering all updated species descriptions. Subsequent extensive phenotypic characterisation, DNA-DNA hybridisation, 16S rRNA gene sequencing and a Multi-Locus Phylogenetic Analysis (MLPA) of the concatenated sequence of 7 housekeeping genes (gyrB, rpoD, recA, dnaJ, gyrA, dnaX and atpD; 4705 bp) was employed in an attempt to ascertain the taxonomy of this isolate. Based on this polyphasic approach, we describe a novel species of the genus Aeromonas, for which the name Aeromonas cavernicola sp. nov. is proposed, with strain CCM7641(T) (DSM24474(T), CECT7862(T)) as the type strain.
authors.affiliation: Area de Microbiología, EPSO, Universidad Miguel Hernández, 03312 Orihuela, Alicante, Spain. ammurcia@geneticpcr.com
authors.forename: Antonio
authors.initials: A
authors.lastname: Martínez-Murcia
authors.forename: Roxana
authors.initials: R
authors.lastname: Beaz-Hidalgo
authors.forename: Pavel
authors.initials: P
authors.lastname: Svec
authors.forename: Ma José
authors.initials: MJ
authors.lastname: Saavedra
authors.forename: Ma José
authors.initials: MJ
authors.lastname: Figueras
authors.forename: Ivo
authors.initials: I
authors.lastname: Sedlacek
mesh_terms: D000333:Aeromonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D059552:Caves; D016000:Cluster Analysis; D018153:Czech Republic; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005618:Fresh Water / Q000382:microbiology*; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D058889:Molecular Typing; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S