title: Cloning and characterization of a tuberous root-specific promoter from cassava (Manihot esculenta Crantz).
abstract: In order to obtain a tuberous root-specific promoter to be used in the transformation of cassava, a 1,728 bp sequence containing the cassava granule-bound starch synthase (GBSSI) promoter was isolated. The sequence proved to contain light- and sugar-responsive cis elements. Part of this sequence (1,167 bp) was cloned into binary vectors to drive expression of the firefly luciferase gene. Cassava cultivar Adira 4 was transformed with this construct or a control construct in which the luciferase gene was cloned behind the 35S promoter. Luciferase activity was measured in leaves, stems, roots and tuberous roots. As expected, the 35S promoter induced luciferase activity in all organs at similar levels, whereas the GBSSI promoter showed very low expression in leaves, stems and roots, but very high expression in tuberous roots. These results show that the cassava GBSSI promoter is an excellent candidate to achieve tuberous root-specific expression in cassava.
authors.affiliation: Wageningen UR Plant Breeding, Wageningen University and Research Center, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands. herma.koehorst@wur.nl
authors.forename: Herma J J
authors.initials: HJ
authors.lastname: Koehorst-van Putten
authors.forename: Anne-Marie A
authors.initials: AM
authors.lastname: Wolters
authors.forename: Isolde M
authors.initials: IM
authors.lastname: Pereira-Bertram
authors.forename: Hans H J
authors.initials: HH
authors.lastname: van den Berg
authors.forename: Alexander R
authors.initials: AR
authors.lastname: van der Krol
authors.forename: Richard G F
authors.initials: RG
authors.lastname: Visser
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D002365:Manihot / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D018519:Meristem / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D008969:Molecular Sequence Data; D009928:Organ Specificity; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D018547:Plant Stems / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D035281:Plant Tubers / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013214:Starch Synthase / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D008156:Luciferases; D013214:Starch Synthase