title: ssDNA-dsRNAs are cleaved at the next to its chimera-junction point by an unknown RNase activity.
abstract: We found that there is an unknown aspect in serum RNases that cleaves ssDNA-dsRNA and ssRNA-dsRNA. In the first step, RNase cleaves the phosphodiester linkage between the first and second RNA, where the first one is connected to the single stranded RNA or DNA. In the second step, the ssRNA overhang attached siRNA is cleaved. When the 2' hydroxyl of the first RNA was replaced with methoxy, the cleavage did not occur. This RNase activity can be considered related to defense system against exogenous genetic materials.
authors.affiliation: Department of Chemistry and Biochemistry, The University of Kitakyushu, 1-1, Hibikino, Kitakyushu, Fukuoka 808-0135, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Mochizuki
authors.forename: Sadaharu
authors.initials: S
authors.lastname: Higuchi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sakurai
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D053837:DNA Cleavage; D006801:Humans; D051379:Mice; D059367:RNA Cleavage; D012330:RNA, Double-Stranded / Q000737:chemistry*; D012260:Ribonucleases / Q000737:chemistry*; D044967:Serum / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D012330:RNA, Double-Stranded; D004247:DNA; D012260:Ribonucleases