title: Induced apoptosis of Th2 lymphocytes and inhibition of airway hyperresponsiveness and inflammation by combined lactic acid bacteria treatment.
abstract: Several lactic acid bacteria (LAB) demonstrably regulate the immune system and inhibit allergic disease. This study examined whether oral feeding of either Lactobacillus paracasei (L. paracasei) BB5 and/or Lactobacillus rhamnosus (L. rhamnosus) BB1 suppresses ovalbumin (OVA)-induced airway hyperresponsiveness (AHR) and inflammation in a murine model. OVA-specific immune responses, cell profile of bronchoalveolar lavage fluid (BALF), and airway AHR were assessed following OVA and methacholine challenge. We investigated whether LAB can enhance CD4(+)FoxP3(+) and CD8(+)FoxP3(+) regulatory T (Treg) cells in splenic cells and apoptosis of CD4(+)IL-4(+) T cells. Results found oral administration of combined LAB better than single L. paracasei or L. rhamnosus strain, improving Penh ratio after challenge with methacholine. High-dose combined LAB starkly decreased synthesis of OVA-specific IgE and IgG2a levels, as well as eosinophils infiltration in BALF. In addition, CD4(+)IL-4(+) T cells decreased while CD4(+)FoxP3(+) and CD8(+)FoxP3(+) Treg cells increased significantly in splenic mononuclear cells of high-dose combined LAB group. Findings indicate allergen-induced AHR and airway allergic inflammation suppressed by enhances CD4(+)FoxP3(+) and CD8(+)FoxP3(+) Treg populations as well as Th1 cell response after treating with combined LAB. This study may provide a basis for developing a novel therapeutic or protective method for airway allergic disease.
authors.affiliation: School of Pharmacy, China Medical University, Taichung, Taiwan.
authors.forename: Wen-Hsin
authors.initials: WH
authors.lastname: Lin
authors.forename: Chi-Rei
authors.initials: CR
authors.lastname: Wu
authors.forename: Hong-Zin
authors.initials: HZ
authors.lastname: Lee
authors.forename: Yueh-Hsiung
authors.initials: YH
authors.lastname: Kuo
authors.forename: Hung-Shin
authors.initials: HS
authors.lastname: Wen
authors.forename: Tze-Yi
authors.initials: TY
authors.lastname: Lin
authors.forename: Chia-Ying
authors.initials: CY
authors.lastname: Lee
authors.forename: Shi-Ying
authors.initials: SY
authors.lastname: Huang
authors.forename: Ching-Yuang
authors.initials: CY
authors.lastname: Lin
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000276:immunology*; D001249:Asthma / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D007136:Immunoglobulins / Q000097:blood; D007167:Immunotherapy / Q000379:methods; D007778:Lactobacillus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D011014:Pneumonia / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology* / Q000473:pathology; D014132:Trachea / Q000276:immunology / Q000473:pathology
chemical_list: D016207:Cytokines; D007136:Immunoglobulins; D010047:Ovalbumin