title: The RNA-binding protein MARTA2 regulates dendritic targeting of MAP2 mRNAs in rat neurons.
abstract: Dendritic targeting of mRNAs encoding the microtubule-associated protein 2 (MAP2) in neurons involves a cis-acting dendritic targeting element. Two rat brain proteins, MAP2-RNA trans-acting protein (MARTA)1 and MARTA2, bind to the cis-element with both high affinity and specificity. In this study, affinity-purified MARTA2 was identified as orthologue of human far-upstream element binding protein 3. In neurons, it resides in somatodendritic granules and dendritic spines and associates with MAP2 mRNAs. Expression of a dominant-negative variant of MARTA2 disrupts dendritic targeting of endogenous MAP2 mRNAs, while not noticeably altering the level and subcellular distribution of polyadenylated mRNAs as a whole. Finally, MAP2 transcripts associate with the microtubule-based motor KIF5 and inhibition of KIF5, but not cytoplasmic dynein function disrupts extrasomatic trafficking of MAP2 mRNA granules. Thus, in neurons MARTA2 appears to represent a key trans-acting factor involved in KIF5-mediated dendritic targeting of MAP2 mRNAs.
authors.affiliation: Institute for Human Genetics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Krishna H
authors.initials: KH
authors.lastname: Zivraj
authors.forename: Monika
authors.initials: M
authors.lastname: Rehbein
authors.forename: Janin
authors.initials: J
authors.lastname: Ölschläger-Schütt
authors.forename: Claudia
authors.initials: C
authors.lastname: Schob
authors.forename: Katrin
authors.initials: K
authors.lastname: Falley
authors.forename: Friedrich
authors.initials: F
authors.lastname: Buck
authors.forename: Michaela
authors.initials: M
authors.lastname: Schweizer
authors.forename: Antonino
authors.initials: A
authors.lastname: Schepis
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Kremmer
authors.forename: Dietmar
authors.initials: D
authors.lastname: Richter
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Kreienkamp
authors.forename: Stefan
authors.initials: S
authors.lastname: Kindler
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003712:Dendrites / Q000378:metabolism* / Q000648:ultrastructure; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D017403:In Situ Hybridization; D013058:Mass Spectrometry; D016253:Microscopy, Immunoelectron; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D021381:Protein Transport / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C579810:Fubp3 protein, rat; C489787:MAP2 protein, rat; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins