title: Langmuir-Blodgett nanotemplate and radiation resistance in protein crystals: state of the art.
abstract: A state-of-the-art review of the role of the Langmuir-Blodgett nanotemplate on protein crystal structures is here presented. Crystals grown by nanostructured template appear more radiation resistant than the classical ones, even in the presence of a third-generation highly focused beam at the European Synchrotron Radiation Facility. The electron density maps and the changes in parameters such as total diffractive power, B-factor, and pairwise R-factor have been discussed. Protein crystals, grown by the Langmuir-Blodgett nanotemplate-based method, proved to be more radiation resistant compared to crystals grown by the classical hanging drop method in terms of both global and specific damage.
authors.affiliation: Nanoworld Institute, Fondazione EL.B.A. Nicolini, Largo Redaelli 7, Pradalunga, Bergamo and Laboratories of Biophysics and Nanobiotechnology, Department of Experimental Medicine, University of Genova, Via Pastore 3, 16132 Genova Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Belmonte
authors.forename: Eugenia
authors.initials: E
authors.lastname: Pechkova
authors.forename: Shailesh
authors.initials: S
authors.lastname: Tripathi
authors.forename: Dora
authors.initials: D
authors.lastname: Scudieri
authors.forename: Claudio
authors.initials: C
authors.lastname: Nicolini
mesh_terms: D000249:Adenosine Monophosphate / Q000031:analogs & derivatives / Q000737:chemistry / Q000528:radiation effects; D003460:Crystallization / Q000379:methods*; D004307:Dose-Response Relationship, Radiation; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D011506:Proteins / Q000737:chemistry / Q000528:radiation effects; D011827:Radiation; D017356:Synchrotrons / Q000295:instrumentation; D013762:Tetrahydrofolate Dehydrogenase / Q000737:chemistry / Q000528:radiation effects
chemical_list: D011506:Proteins; C006094:R388; D000249:Adenosine Monophosphate; C044533:3'-(1-butylphosphoryl)adenosine; D013762:Tetrahydrofolate Dehydrogenase