title: Localization of estrogen receptor in the central lymphoid organs of chickens during the late stage of embryogenesis.
abstract: Immunological function in chicks is greatly affected by estrogen treatment during embryogenesis, but the mechanism of the estrogen effect is not fully understood. To elucidate the effect of estrogen on immune function, we observed estrogen receptor expression in the thymus and bursa of chick embryos by immunohistochemistry. We compared the distribution of estrogen receptor-positive cells with that of keratin-positive epithelial cells. Intense expression of estrogen receptors was detected in thymic and bursal lymphocytes. In peripheral lymphocytes, ER mRNA was detected by RT-PCR analysis. The results of fluorescence-activated cell sorting analysis indicated that the estrogen receptor was expressed in the cytoplasm of the lymphocytes. Furthermore, intense expression of the estrogen receptor was also confirmed in thymic Hassall's corpuscles, bursal follicle-associated epithelial cells, and the bursal interfollicular epithelium. Our results indicate that estrogen affects the differentiation of thymic and bursal lymphocytes, suggesting that the underlying role for estrogen in immune function.
authors.affiliation: Laboratory of Applied Functional Anatomy of Animals, Faculty of Agriculture, Okayama University, Okayama 700-8530, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Katayama
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Fukuda
authors.forename: Kiyoaki
authors.initials: K
authors.lastname: Narabara
authors.forename: Asaki
authors.initials: A
authors.lastname: Abe
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kondo
mesh_terms: D000818:Animals; D002060:Bursa of Fabricius / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics / Q000378:metabolism*; D047108:Embryonic Development; D018507:Gene Expression Regulation, Developmental; D008214:Lymphocytes / Q000378:metabolism; D009928:Organ Specificity; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D013154:Spleen / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D011960:Receptors, Estrogen