title: Real-time monitoring of protein conformational dynamics in solution using kinetic capillary electrophoresis.
abstract: Conformational analysis: Capillary electrophoresis (CE) allows for the rapid separation of slowly interconverting protein conformers. Kinetic analysis (k(open), k(closed), and K(d)) of electropherograms in the presence and absence of effector ligands allows the measurement of kinetic and thermodynamic constants associated with conformational changes and ligand binding.
authors.affiliation: Department of Chemistry, University of Ottawa, 10 Marie Curie, Ottawa, K1N 6N5, Canada.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Clouthier
authors.forename: Gleb G
authors.initials: GG
authors.lastname: Mironov
authors.forename: Victor
authors.initials: V
authors.lastname: Okhonin
authors.forename: Maxim V
authors.initials: MV
authors.lastname: Berezovski
authors.forename: Jeffrey W
authors.initials: JW
authors.lastname: Keillor
mesh_terms: D000494:Allosteric Regulation; D002118:Calcium / Q000737:chemistry; D019075:Electrophoresis, Capillary; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D006153:Guanosine Diphosphate / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008024:Ligands; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D012996:Solutions / Q000737:chemistry*; D013816:Thermodynamics; D011503:Transglutaminases / Q000737:chemistry / Q000378:metabolism
chemical_list: D008024:Ligands; D011506:Proteins; D012996:Solutions; D006153:Guanosine Diphosphate; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D019204:GTP-Binding Proteins; D002118:Calcium