title: Evaluation of turbidimetric inhibition immunoassay (TINIA) and HPLC methods for glycated haemoglobin determination.
abstract: BACKGROUND
  Various factors may affect the accuracy of hemoglobin (Hb) A1c measurements that are widely used to monitor glycemic control in diabetic patients. This study was aimed to compare the values of HbA1c obtained by two different methods, Roche Tina-quant second and thirdgeneration HbA1c assays based on the turbidimetric inhibition immunoassay (TINIA), and high-performance liquid chromatography (HPLC) cation-exchange method used by Arkray Adams HA-8160 analyzer.
  
  
  METHODS
  Measurements of HbA1c were carried out in blood samples from 2,917 patients using above-mentioned methods. Linear regression was used for the correlation analysis and linear equations. Bland-Altman plots were performed from method comparison data using MedCalc statistical software.
  
  
  RESULTS
  For the low control, the second generation Tina-quant assay had within-run and between-run CVs 0.8% and 0.9%; for the high control within-run and between-run CVs were 1% and 0.96%, respectively. HPLC method for the low control had within-run CV 1% and between-run CV 1.3%; for the high control within-run CV was 0.6% and between-run CV was 0.9%.
  
  
  CONCLUSIONS
  There was a good concordance between the results of TINIA and HPLC methods (y = 1.091x - 0.363; r(2)  = 0.96).
authors.affiliation: Istanbul Faculty of Medicine, Department of Biochemistry, Istanbul University, Istanbul, Turkey.
authors.forename: Sema
authors.initials: S
authors.lastname: Genc
authors.forename: Beyhan
authors.initials: B
authors.lastname: Omer
authors.forename: Esra
authors.initials: E
authors.lastname: Aycan-Ustyol
authors.forename: Nurhan
authors.initials: N
authors.lastname: Ince
authors.forename: Fatih
authors.initials: F
authors.lastname: Bal
authors.forename: Figen
authors.initials: F
authors.lastname: Gurdol
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D016014:Linear Models; D009391:Nephelometry and Turbidimetry; D015203:Reproducibility of Results
chemical_list: D006442:Glycated Hemoglobin