title: Oxidative DNA damage of peripheral blood polymorphonuclear leukocytes, selectively induced by chronic arsenic exposure, is associated with extent of arsenic-related skin lesions.
abstract: There is increasing evidence that oxidative stress is an important risk factor for arsenic-related diseases. Peripheral blood leukocytes constitute an important defense against microorganisms or pathogens, while the research on the impact of chronic arsenic exposure on peripheral blood leukocytes is much more limited, especially at low level arsenic exposure. The purpose of the present study was to explore whether chronic arsenic exposure affects oxidative stress of peripheral blood leukocytes and possible linkages between oxidative stress and arsenic-induced skin lesions. 75 male inhabitants recruited from an As-endemic region of China were investigated in the present study. The classification of arsenicosis was based on the degree of skin lesions. Arsenic levels were measured in drinking water and urine by Atomic Fluorescence Spectroscopy. Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) was tested by Enzyme-Linked Immunosorbent Assay. 8-OHdG of peripheral blood leukocytes was evaluated using immunocytochemical staining. 8-OHdG-positive reactions were only present in polymorphonuclear leukocytes (PMNs), but not in monocytes (MNs). The 8-OHdG staining of PMN cytoplasm was observed in all investigated populations, while the 8-OHdG staining of PMN nuclei was frequently found along with the elevated amounts of cell debris in individuals with skin lesion. Urinary arsenic levels were increased in the severe skin lesion group compared with the normal group. No relationship was observed between drinking water arsenic or urine 8-OHdG and the degree of skin lesions. These findings indicated that the target and persistent oxidative stress in peripheral blood PMNs may be employed as a sensitive biomarker directly to assess adverse health effects caused by chronic exposure to lower levels of arsenic.
authors.affiliation: Department of Toxicology, Public Health College, Shanxi Medical University, No 56 Xin Jian Nan Lu, Taiyuan (030001), China. 924969007@qq.com
authors.forename: Qiuling
authors.initials: Q
authors.lastname: Pei
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Wenchao
authors.initials: W
authors.lastname: Xu
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Ma
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Li
authors.forename: Fengjie
authors.initials: F
authors.lastname: Tian
authors.forename: Wenping
authors.initials: W
authors.lastname: Zhang
authors.forename: Jinjun
authors.initials: J
authors.lastname: Mu
authors.forename: Yuanfei
authors.initials: Y
authors.lastname: Li
authors.forename: Dongxing
authors.initials: D
authors.lastname: Wang
authors.forename: Haifang
authors.initials: H
authors.lastname: Liu
authors.forename: Mimi
authors.initials: M
authors.lastname: Yang
authors.forename: Caifeng
authors.initials: C
authors.lastname: Ma
authors.forename: Fen
authors.initials: F
authors.lastname: Yun
mesh_terms: D000328:Adult; D000368:Aged; D001151:Arsenic / Q000008:administration & dosage*; D004249:DNA Damage / Q000187:drug effects / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D012878:Skin Neoplasms / Q000139:chemically induced* / Q000453:epidemiology / Q000378:metabolism*; D014877:Water Pollution, Chemical / Q000009:adverse effects*; D014881:Water Supply / Q000032:analysis; D055815:Young Adult
chemical_list: D001151:Arsenic