title: Multiple sodium channel variants in the mosquito Culex quinquefasciatus.
abstract: Voltage-gated sodium channels are the target sites of both DDT and pyrethroid insecticides. The importance of alternative splicing as a key mechanism governing the structural and functional diversity of sodium channels and the resulting development of insecticide and acaricide resistance is widely recognized, as shown by the extensive research on characterizing alternative splicing and variants of sodium channels in medically and agriculturally important insect species. Here we present the first comparative study of multiple variants of the sodium channel transcripts in the mosquito Culex quinquefasciatus. The variants were classified into two categories, CxNa-L and CxNa-S based on their distinguishing sequence sizes of ~6.5 kb and ~4.0 kb, respectively, and generated via major extensive alternative splicing with minor small deletions/ insertions in susceptible S-Lab, low resistant HAmCq(G0), and highly resistant HAmCq(G8)Culex strains. Four alternative Cx-Na-L splice variants were identified, including three full length variants with three optional exons (2, 5, and 21i) and one with in-frame-stop codons. Large, multi-exon-alternative splices were identified in the CxNa-S category. All CxNa-S splicing variants in the S-Lab and HAmCq(G0) strains contained in-frame stop codons, suggesting that any resulting proteins would be truncated. The ~1000 to ~3000-fold lower expression of these splice variants with stop codons compared with the CxNa-L splicing variants may support the lower importance of these variants in S-Lab and HAmCq(G0). Interestingly, two alternative splicing variants of CxNa-S in HAmCq(G8) included entire ORFs but lacked exons 5 to18 and these two variants had much higher expression levels in HAmCq(G8) than in S-Lab and HAmCq(G0). These results provide a functional basis for further characterizing how alternative splicing of a voltage-gated sodium channel contributes to diversity in neuronal signaling in mosquitoes in response to pyrethroids, and possibly indicates the role of these variants in the development of pyrethroid resistance.
authors.affiliation: Department of Entomology and Plant Pathology, Auburn University, Auburn, AL 36849, USA.
authors.forename: Lin
authors.initials: L
authors.lastname: He
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.forename: Lee
authors.initials: L
authors.lastname: Zhang
authors.forename: Nannan
authors.initials: N
authors.lastname: Liu
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D003465:Culex / Q000378:metabolism*; D007305:Insecticide Resistance / Q000235:genetics; D007306:Insecticides; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011722:Pyrethrins; D012333:RNA, Messenger / Q000737:chemistry; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D017423:Sequence Analysis, RNA; D061566:Voltage-Gated Sodium Channels / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D007306:Insecticides; D011722:Pyrethrins; D012333:RNA, Messenger; D061566:Voltage-Gated Sodium Channels
keywords: Culex quinquefasciatus.; Sodium channel; alternative splicing; insecticide resistance; transcript variants