title: Improving PCR detection of prey in molecular diet studies: importance of group-specific primer set selection and extraction protocol performances.
abstract: While the morphological identification of prey remains in predators' faeces is the most commonly used method to study trophic interactions, many studies indicate that this method does not detect all consumed prey. Polymerase chain reaction-based methods are increasingly used to detect prey DNA in the predator food bolus and have proven efficient, delivering highly accurate results. When studying complex diet samples, the extraction of total DNA is a critical step, as polymerase chain reaction (PCR) inhibitors may be co-extracted. Another critical step involves a careful selection of suitable group-specific primer sets that should only amplify DNA from the targeted prey taxon. In this study, the food boluses of five Rattus rattus and seven Rattus exulans were analysed using both morphological and molecular methods. We tested a panel of 31 PCR primer pairs targeting bird, invertebrate and plant sequences; four of them were selected to be used as group-specific primer pairs in PCR protocols. The performances of four DNA extraction protocols (QIAamp(®)  DNA stool mini kit, DNeasy(®)  mericon food kit and two of cetyltrimethylammonium bromide-based methods) were compared using four variables: DNA concentration, A(260) /A(280)  absorbance ratio, food compartment analysed (stomach or faecal contents) and total number of prey-specific PCR amplification per sample. Our results clearly indicate that the A(260) /A(280)  absorbance ratio, which varies between extraction protocols, is positively correlated to the number of PCR amplifications of each prey taxon. We recommend using the DNeasy(®)  mericon food kit (QIAGEN), which yielded results very similar to those achieved with the morphological approach.
authors.affiliation: Aix-Marseille Université, CNRS, IRD, IMBE - UMR 7263, Aix-en-Provence Cedex 04, France. diane.zarzoso-lacoste@imbe.fr
authors.forename: Diane
authors.initials: D
authors.lastname: Zarzoso-Lacoste
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Corse
authors.forename: Eric
authors.initials: E
authors.lastname: Vidal
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D005243:Feces / Q000737:chemistry*; D020387:Food Chain; D005766:Gastrointestinal Contents / Q000737:chemistry*; D008853:Microscopy; D008969:Molecular Sequence Data; D010139:Pacific Islands; D016133:Polymerase Chain Reaction / Q000379:methods*; D051381:Rats; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D004247:DNA