title: Burkholderia denitrificans sp. nov., isolated from the soil of Dokdo Island, Korea.
abstract: A novel, Gram-negative, bacterial strain KIS30-44(T) was identified from wet forest soil collected on the Korean island of Dokdo. Growth of the strain was observed at 15-30°C, pH 5-9, 0-3% NaCl, and 950 mM KNO(3). KIS30-44(T) reduced nitrate to nitrogen gas. Analysis of the 16S rRNA gene sequence showed that KIS30-44(T) was phylogenetically related to Burkholderia sacchari, Burkholderia mimosarum, and Burkholderia oxyphila (98.1%, 98.0%, and 98.0% sequence similarity, respectively). The genomic G+C content was 63.5 mol%. KIS30-44(T) exhibited less than 52% DNA-DNA relatedness with the type strains of 9 closely related Burkholderia species. The major isoprenoid quinone was Q-8. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unknown aminolipids. The major fatty acids in KIS30-44(T) were C(16:0), C(18:1)                 ω7c and summed feature 3 (iso-C(15:0) 2-OH and C(16:1)                 ω7c), and the strain contained half the amount of C(17:0) cyclo found in the 9 closely related Burkholderia species. The results of these phenotypic, 16S rRNA gene sequence, DNA-DNA hybridization, and chemotaxonomic data indicate that KIS30-44(T) represents a novel species within the genus Burkholderia, for which the name Burkholderia denitrificans (Type strain KIS30-44(T) =KACC 12733(T) =DSM 24336(T)) is proposed.
authors.affiliation: Functional Biomaterial Division, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Chang-Muk
authors.initials: CM
authors.lastname: Lee
authors.forename: Hang-Yeon
authors.initials: HY
authors.lastname: Weon
authors.forename: Sang-Hong
authors.initials: SH
authors.lastname: Yoon
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Bon-Sung
authors.initials: BS
authors.lastname: Koo
authors.forename: Soon-Wo
authors.initials: SW
authors.lastname: Kwon
mesh_terms: D001482:Base Composition; D019117:Burkholderia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S