title: Periprostatic implantation of human bone marrow-derived mesenchymal stem cells potentiates recovery of erectile function by intracavernosal injection in a rat model of cavernous nerve injury.
abstract: OBJECTIVE
  To evaluate whether periprostatic implantation (PPI) of human bone marrow-derived mesenchymal stem cells (hBMSCs) potentiates recovery of erectile function after intracavernosal injection (ICI) of hBMSCs in a rat model of cavernous nerve (CN) injury.
  
  
  METHODS
  Sprague-Dawley rats that had undergone bilateral CN injury were treated by ICI with or without PPI of hBMSCs (10 rats per group). hBMSCs were harvested from healthy human donors. Fibrin scaffolds were used for PPI of hBMSCs. After 4 weeks, erectile responses to electric pelvic ganglion stimulation were studied. The expression of neuronal nitric oxide synthase (nNOS)-positive nerve fibers and smooth muscle/collagen ratio was evaluated in each penis.
  
  
  RESULTS
  ICI of hBMSCs slightly improved erectile function compared with the control group (maximal intracavernosal pressure/mean arterial pressure, 39.1% vs 21.7%; P=.060), but a combination of PPI and ICI significantly improved erectile function (45.0%, P=.007). After stem cell therapy, the number of nNOS-positive nerve fibers increased significantly in the PPI+ICI group (P=.017). The smooth muscle/collagen ratio increased significantly after stem cell therapy in the ICI and PPI+ICI groups (both P<.001).
  
  
  CONCLUSIONS
  ICI of hBMSCs in a rat model of CN injury results in recovery of penile erection by decreasing corporeal smooth muscle deterioration and collagen deposition. PPI of hBMSCs potentiates recovery of erectile function by ICI of hBMSCs via regeneration of nNOS-containing nerve fibers.
authors.affiliation: Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Dalsan
authors.initials: D
authors.lastname: You
authors.forename: Myoung Jin
authors.initials: MJ
authors.lastname: Jang
authors.forename: Jiyeon
authors.initials: J
authors.lastname: Lee
authors.forename: In Gab
authors.initials: IG
authors.lastname: Jeong
authors.forename: Hyun Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Kyung Hyun
authors.initials: KH
authors.lastname: Moon
authors.forename: Nayoung
authors.initials: N
authors.lastname: Suh
authors.forename: Choung-Soo
authors.initials: CS
authors.lastname: Kim
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D003094:Collagen / Q000378:metabolism; D004195:Disease Models, Animal; D004558:Electric Stimulation; D007172:Erectile Dysfunction / Q000503:physiopathology / Q000628:therapy*; D005724:Ganglia / Q000378:metabolism / Q000503:physiopathology; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D009416:Nerve Regeneration; D009474:Neurons / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D010410:Penile Erection; D010413:Penis / Q000294:innervation* / Q000378:metabolism / Q000503:physiopathology; D059348:Peripheral Nerve Injuries / Q000209:etiology / Q000628:therapy*; D011468:Prostatectomy / Q000009:adverse effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function
chemical_list: D003094:Collagen; D019001:Nitric Oxide Synthase