title: Textural characteristics of model and natural bone tissues and interfacial behavior of bound water.
abstract: Water, as a probe liquid bound in model systems (highly disperse hydroxyapatite - protein composites as a model of the main components of bones) and rat bone tissues healthy and affected by osteoporosis occurred due to experimental Alzheimer's disease (EAD), has been investigated using low-temperature (1)H NMR spectroscopy, NMR cryoporometry, TG/DTG/DTA, DSC, and TG and DSC thermoporometry. The textural characteristics of these intact systems cannot be studied using the standard adsorption methods, but the cryoporometry and thermoporometry methods give these characteristics. The (1)H NMR spectra of water bound in model and natural bone tissues include signals, which can be assigned to strongly associated (typical) water (SAW, chemical shift of proton resonance δ(H)=5-6 ppm) and weakly associated (atypical) water (WAW) at δ(H)=1-2 ppm. Contributions of SAW and WAW give information on textural organization of both model and natural bones. The influence of such co-adsorbates as HCl, CDCl(3), CD(3)CN, C(6)D(6), and (CD(3))(2)SO on the interfacial behavior and clustering of bound water depends on their polarity, amounts of components, and textural and structural features of the materials analyzed with the (1)H NMR spectroscopy and cryoporometry methods. According to the NMR cryoporometry data, the EAD causes an increase in nanoporosity of the bone tissues. The total porosity and the specific surface area of biostructures (accessible for water molecules and estimated using NMR cryoporometry and TG thermoporometry methods with a model of cylindrical pores) are larger for the EAD sample. Weakly polar chloroform-d has a significant influence on the organization of water in the bone tissue, and this effect is greater for the EAD sample as more porous material.
authors.affiliation: Department of Orthopaedics and Rehabilitation, Lublin Medical University, 20-094 Lublin, Poland.
authors.forename: Miroslaw
authors.initials: M
authors.lastname: Jabłoński
authors.affiliation: Chuiko Institute of Surface Chemistry, 17 General Naumov Street, 03164 Kiev, Ukraine. Electronic address: vlad_gunko@ukr.net.
authors.forename: Vladimir M
authors.initials: VM
authors.lastname: Gun'ko
authors.affiliation: Chuiko Institute of Surface Chemistry, 17 General Naumov Street, 03164 Kiev, Ukraine.
authors.forename: Alina P
authors.initials: AP
authors.lastname: Golovan
authors.affiliation: Faculty of Chemistry, Maria Curie-Sklodowska University, 20-031 Lublin, Poland.
authors.forename: Roman
authors.initials: R
authors.lastname: Leboda
authors.affiliation: Faculty of Chemistry, Maria Curie-Sklodowska University, 20-031 Lublin, Poland.
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Skubiszewska-Zięba
authors.affiliation: Laboratory of Ischemic and Neurodegenerative Brain Research, Department of Neurodegenerative Disorders, Mossakowski Medical Research Centre, Polish Academy of Sciences, 02-106 Warsaw, Poland.
authors.forename: Ryszard
authors.initials: R
authors.lastname: Pluta
authors.affiliation: Chuiko Institute of Surface Chemistry, 17 General Naumov Street, 03164 Kiev, Ukraine.
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Turov
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000737:chemistry*; D017886:Durapatite / Q000138:chemical synthesis / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D010316:Particle Size; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties; D013696:Temperature; D014867:Water / Q000737:chemistry*
chemical_list: D014867:Water; D017886:Durapatite