title: Effect of exogenous testosterone on oxidative status of the testes in adult male rats.
abstract: The aim of this study was to investigate the testosterone-induced changes in the oxidative status of testes in adult male rats treated either with testosterone or after blockade of androgen receptors with cyproterone acetate. A total of 40 intact rats were divided into four groups: a control group receiving sterile oil, the testosterone group receiving testosterone isobutyras, the cyproterone group receiving cyproterone acetate and the combination group receiving both testosterone isobutyras and cyproterone acetate. Treatments were carried out for 2 days by intramuscular application. Parameters of oxidative stress and the expression levels of the steroidogenic acute regulatory protein (StAR) gene were measured in testes. Significantly increased TBARS and advanced glycation end products (AGEs) levels were found in the testosterone group when compared to the control group. The °1 ferric-reducing ability of the tissue and total antioxidative capacity were lower in the testosterone group in comparison with the control group. Gene expression analysis revealed significant downregulation of the StAR gene in the testes of rats in the testosterone and combination groups with respect to control animals. In conclusion, administration of exogenous testosterone influences the lipid peroxidation and carbonyl stress and decreases the antioxidant defence in the testes. These data might have implications for male fertility in humans.
authors.affiliation: Institute of Molecular Biomedicine, Comenius University, Bratislava, Slovakia.
authors.forename: L
authors.initials: L
authors.lastname: Tóthová
authors.forename: P
authors.initials: P
authors.lastname: Celec
authors.forename: D
authors.initials: D
authors.lastname: Ostatníková
authors.forename: M
authors.initials: M
authors.lastname: Okuliarová
authors.forename: M
authors.initials: M
authors.lastname: Zeman
authors.forename: J
authors.initials: J
authors.lastname: Hodosy
mesh_terms: D059002:Androgen Receptor Antagonists / Q000494:pharmacology; D000818:Animals; D017373:Cyproterone Acetate / Q000494:pharmacology; D015536:Down-Regulation; D017127:Glycation End Products, Advanced / Q000187:drug effects; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D013739:Testosterone / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D059002:Androgen Receptor Antagonists; D017127:Glycation End Products, Advanced; D010750:Phosphoproteins; D017392:Thiobarbituric Acid Reactive Substances; D000098682:Steroidogenic Acute Regulatory Protein; D013739:Testosterone; D017373:Cyproterone Acetate; C028499:testosterone isobutyrate
keywords: Exogenous testosterone; infertility; oxidative stress; steroidogenic acute regulatory protein; testes