title: (1)H NMR spectroscopy for the in vitro understanding of the glycaemic index.
abstract: The glycaemic index (GI) characterises foods by using the incremental area under the glycaemic response curve relative to the same amount of oral glucose. Its ability to differentiate between curves of different shapes, the peak response and other aspects of the glycaemic response is contentious. The present pilot study aimed to explore the possibility of using 1H NMR spectroscopy to better understand in vivo digestion characteristics as reflected in the glycaemic response of carbohydrate-rich foods; such an approach might be an adjunct to the in vivo GI test. The glycaemic response of two types of raw wheat flour (2005 from Griffith NSW, Chara, Row 10, Plot 6:181 and store-bought Colese Plain Flour) and a cooked store-bought flour was tested and compared with results recorded during the in vitro enzymatic digestion of the wheat flour samples by glucoamylase from Aspergillus niger (EC 3.2.1.3) as monitored by 1H NMR spectroscopy. Comparing the digestion time courses of raw and cooked wheat starch recorded in vitro strongly suggests that the initial rate of glucose release in vitro correlates with the glycaemic spike in vivo. During the in vitro time courses, approximately four times as much glucose was released from cooked starch samples than from raw starch samples in 90 min. Monitoring enzymatic digestion of heterogeneous mixtures (food) by 1H NMR spectroscopy showcases the effectiveness of the technique in measuring glucose release and its potential use as the basis of an in vitro method for a better understanding of the GI.
authors.affiliation: School of Molecular Bioscience, University of Sydney, NSW 2006, Australia.
authors.forename: Anthony C
authors.initials: AC
authors.lastname: Dona
authors.forename: Karola
authors.initials: K
authors.lastname: Landrey
authors.forename: Fiona S
authors.initials: FS
authors.lastname: Atkinson
authors.forename: Jennie C
authors.initials: JC
authors.lastname: Brand Miller
authors.forename: Philip W
authors.initials: PW
authors.lastname: Kuchel
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D001786:Blood Glucose / Q000378:metabolism; D003296:Cooking; D005433:Flour / Q000145:classification; D005504:Food Analysis / Q000379:methods*; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism*; D038321:Glycemic Index; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010865:Pilot Projects; D013213:Starch / Q000378:metabolism*; D014908:Triticum / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D013213:Starch; D005087:Glucan 1,4-alpha-Glucosidase