title: Comparison of the Bactec Fx Plus, Mycosis IC/F, Mycosis/F Lytic blood culture media and the BacT/Alert 3D FA media for detection of Candida species in seeded blood culture specimens containing therapeutic peak levels of fluconazole.
abstract: BACKGROUND
  The performance of Bactec Fx Plus Aerobic/F (PA), Mycosis IC/F (MF), Myco/F Lytic (ML) media and BacT/Alert 3D FA (FA) media in detecting 15 Candida isolates in blood cultures to which fluconazole had been added was investigated.
  
  
  METHODS
  PA with resin, MF, ML media (n = 360), and FAmedia (n = 120) were tested against Candida albicans, C. tropicalis, C. parapsilosis, and C. krusei. As the peak plasma concentration after single oral doses of fluconazole 100, 200, and 400 mg was equivalent to peak level of 1.9, 4.7, and 6.7 mg/l, respectively, corresponding fluconazole was added. Time to detection (TTD) was measured.
  
  
  RESULTS
  Overall TTD (mean hour ± standard deviation) for PA, FA, MF, and ML was as follows: 24.5 ± 7.3, 27.0 ± 7.5, 31.9 ± 21.3, and 37.7 ± 30.1, respectively. TTD of PA was shorter compared to other media. The effect of fluconazole was limited in PA and FA, but MF and ML showed delayed TTD. Larger inoculum size showed shorter TTDin PA and FA.
  
  
  CONCLUSIONS
  TTD of Bactec Fx Plus Aerobic/F was more than 2.5 hr faster among the tested media. As thus system and media are unaffected by added fluconazole, it could be used for the diagnosis of candidemia in the clinical settings including the patients who have been treated empirically with fluconazole at the time when blood cultures were drawn.
authors.affiliation: Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors.forename: Dong Wook
authors.initials: DW
authors.lastname: Jekarl
authors.forename: So-Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Seungok
authors.initials: S
authors.lastname: Lee
authors.forename: Yeon-Joon
authors.initials: YJ
authors.lastname: Park
authors.forename: Jehoon
authors.initials: J
authors.lastname: Lee
authors.forename: Sun Mi
authors.initials: SM
authors.lastname: Baek
authors.forename: Yeon Ju
authors.initials: YJ
authors.lastname: An
authors.forename: Sun Myeong
authors.initials: SM
authors.lastname: Ock
authors.forename: Mi-Kyung
authors.initials: MK
authors.lastname: Lee
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D057205:Automation, Laboratory; D002175:Candida / Q000187:drug effects / Q000302:isolation & purification*; D058387:Candidemia / Q000097:blood* / Q000382:microbiology*; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry*; D015725:Fluconazole / Q000494:pharmacology*; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods; D018709:Statistics, Nonparametric; D013997:Time Factors
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; D015725:Fluconazole