title: Analysis of tomato gene promoters activated in syncytia induced in tomato and potato hairy roots by Globodera rostochiensis.
abstract: The potato cyst nematode (Globodera rostochiensis) induces feeding sites (syncytia) in tomato and potato roots. In a previous study, 135 tomato genes up-regulated during G. rostochiensis migration and syncytium development were identified. Five genes (CYP97A29, DFR, FLS, NIK and PMEI) were chosen for further study to examine their roles in plant-nematode interactions. The promoters of these genes were isolated and potential cis regulatory elements in their sequences were characterized using bioinformatics tools. Promoter fusions with the β-glucuronidase gene were constructed and introduced into tomato and potato genomes via transformation with Agrobacterium rhizogenes to produce hairy roots. The analysed promoters displayed different activity patterns in nematode-infected and uninfected transgenic hairy roots.
authors.affiliation: Department of Plant Physiology, Faculty of Agriculture and Biology, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159, 02-776 Warsaw, Poland. anita_wisniewska@sggw.pl
authors.forename: A
authors.initials: A
authors.lastname: Wiśniewska
authors.forename: J
authors.initials: J
authors.lastname: Dąbrowska-Bronk
authors.forename: K
authors.initials: K
authors.lastname: Szafrański
authors.forename: S
authors.initials: S
authors.lastname: Fudali
authors.forename: M
authors.initials: M
authors.lastname: Święcicka
authors.forename: M
authors.initials: M
authors.lastname: Czarny
authors.forename: A
authors.initials: A
authors.lastname: Wilkowska
authors.forename: K
authors.initials: K
authors.lastname: Morgiewicz
authors.forename: J
authors.initials: J
authors.lastname: Matusiak
authors.forename: M
authors.initials: M
authors.lastname: Sobczak
authors.forename: M
authors.initials: M
authors.lastname: Filipecki
mesh_terms: D020121:5' Untranslated Regions; D000818:Animals; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D015726:Giant Cells / Q000469:parasitology; D005966:Glucuronidase / Q000235:genetics; D006790:Host-Parasite Interactions / Q000235:genetics; D018551:Solanum lycopersicum / Q000235:genetics*; D018517:Plant Roots / Q000166:cytology / Q000235:genetics / Q000469:parasitology*; D030821:Plants, Genetically Modified / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011198:Solanum tuberosum / Q000235:genetics* / Q000469:parasitology; D014431:Tylenchoidea / Q000472:pathogenicity*
chemical_list: D020121:5' Untranslated Regions; D003577:Cytochrome P-450 Enzyme System; D005966:Glucuronidase