title: High-frequency hippocampal oscillations activated by optogenetic stimulation of transplanted human ESC-derived neurons.
abstract: After transplantation, individual stem cell-derived neurons can functionally integrate into the host CNS; however, evidence that neurons derived from transplanted human embryonic stem cells (hESCs) can drive endogenous neuronal network activity in CNS tissue is still lacking. Here, using multielectrode array recordings, we report activation of high-frequency oscillations in the β and γ ranges (10-100 Hz) in the host hippocampal network via targeted optogenetic stimulation of transplanted hESC-derived neurons.
authors.affiliation: Del E. Webb Center for Neuroscience, Aging, and Stem Cell Research, Sanford-Burnham Medical Research Institute, La Jolla, California 92037, USA.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Piña-Crespo
authors.forename: Maria
authors.initials: M
authors.lastname: Talantova
authors.forename: Eun-Gyung
authors.initials: EG
authors.lastname: Cho
authors.forename: Walid
authors.initials: W
authors.lastname: Soussou
authors.forename: Nima
authors.initials: N
authors.lastname: Dolatabadi
authors.forename: Scott D
authors.initials: SD
authors.lastname: Ryan
authors.forename: Rajesh
authors.initials: R
authors.lastname: Ambasudhan
authors.forename: Scott
authors.initials: S
authors.lastname: McKercher
authors.forename: Karl
authors.initials: K
authors.lastname: Deisseroth
authors.forename: Stuart A
authors.initials: SA
authors.lastname: Lipton
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D053595:Embryonic Stem Cells / Q000166:cytology / Q000637:transplantation*; D005260:Female; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D006801:Humans; D008297:Male; D058953:Neural Stem Cells / Q000166:cytology / Q000637:transplantation*; D055495:Neurogenesis / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000637:transplantation*; D062308:Optogenetics; D051381:Rats; D017207:Rats, Sprague-Dawley