title: A rapid and simple method for detection of type II restriction endonucleases in cells of bacteria with high activity of nonspecific nucleases.
abstract: In this work we describe a novel, rapid and simple microscale procedure for identification of restriction endonuclease activity in bacteria lysates, which contain high levels of non-specific DNA nucleases.
authors.affiliation: Department of Molecular Evolution, University of Gdańsk, Gdańsk, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Podgórska
authors.forename: Grażyna
authors.initials: G
authors.lastname: Kujawska
authors.forename: Michał
authors.initials: M
authors.lastname: Skurzewski
authors.forename: Olesya
authors.initials: O
authors.lastname: Batsko
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Kaczorowski
mesh_terms: D001419:Bacteria / Q000201:enzymology*; D003851:Deoxyribonucleases / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry / Q000302:isolation & purification*; D013379:Substrate Specificity
chemical_list: D003851:Deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific