title: Electrochemical direct detection of DNA deamination catalyzed by APOBEC3G.
abstract: APOBEC3G catalyzes deamination of cytosines in HIV-1 genome, and restricts the HIV-1 infection. Here, we propose a picomole-scale assay for the detection of DNA deamination catalyzed by APOBEC3G. Our results show the suitability of the developed method for a time course analysis of enzyme-catalyzed DNA modifications.
authors.affiliation: Graduate School of Pharmaceutical Sciences, University of Toyama, Sugitani 2630, Toyama 930-0194, Japan. chiba@pha.u-toyama.ac.jp
authors.forename: Junya
authors.initials: J
authors.lastname: Chiba
authors.forename: Takahide
authors.initials: T
authors.lastname: Kouno
authors.forename: Shun
authors.initials: S
authors.lastname: Aoki
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sato
authors.forename: JingYing
authors.initials: J
authors.lastname: Zhang
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Matsuo
authors.forename: Masahiko
authors.initials: M
authors.lastname: Inouye
mesh_terms: D000071480:APOBEC-3G Deaminase; D055162:Biocatalysis; D003564:Cytidine Deaminase / Q000378:metabolism*; D004247:DNA / Q000032:analysis*; D015342:DNA Probes / Q000737:chemistry; D003641:Deamination; D055664:Electrochemical Techniques; D005296:Ferrous Compounds / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007546:Isoquinolines / Q000737:chemistry; D000075163:Metallocenes
chemical_list: D015342:DNA Probes; D005296:Ferrous Compounds; D007546:Isoquinolines; D000075163:Metallocenes; D006046:Gold; D004247:DNA; D000071480:APOBEC-3G Deaminase; C464924:APOBEC3G protein, human; D003564:Cytidine Deaminase; C039109:isoquinoline; C004998:ferrocene