title: Production of natural fragrance aromatic acids by coexpression of trans-anethole oxygenase and p-anisaldehyde dehydrogenase genes of Pseudomonas putida JYR-1 in Escherichia coli.
abstract: A gene encoding p-anisaldehyde dehydrogenase (PAADH), which catalyzes the oxidation of p-anisaldehyde to p-anisic acid, was identified to be clustered with the trans-anethole oxygenase (tao) gene in  Pseudomonas putida  JYR-1. Heterologously expressed PAADH in  Escherichia coli  catalyzed the oxidation of vanillin, veratraldehyde, and piperonal to the corresponding aromatic acids vanillic acid, veratric acid, and piperonylic acid, respectively. Coexpression of trans-anethole oxygenase (TAO) and PAADH in  E. coli  also resulted in the successful transformation of trans-anethole, isoeugenol, O-methyl isoeugenol, and isosafrole to p-anisic acid, vanillic acid, veratric acid, and piperonylic acid, respectively, which are compounds found in plants as secondary metabolites. Because of the relaxed substrate specificity and high transformation rates by coexpressed TAO and PAADH in  E. coli , the engineered strain has potential to be applied in the fragrance industry.
authors.affiliation: School of Environmental Science and Engineering, and International Environmental Research Center, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea.
authors.forename: Dongfei
authors.initials: D
authors.lastname: Han
authors.forename: Somwang
authors.initials: S
authors.lastname: Kurusarttra
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Ryu
authors.forename: Robert A
authors.initials: RA
authors.lastname: Kanaly
authors.forename: Hor-Gil
authors.initials: HG
authors.lastname: Hur
mesh_terms: D000081248:Allylbenzene Derivatives; D000595:Amino Acid Sequence; D000840:Anisoles / Q000378:metabolism; D001547:Benzaldehydes / Q000378:metabolism; D001565:Benzoates / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods*; D062425:Hydroxybenzoate Ethers / Q000378:metabolism; D062385:Hydroxybenzoates / Q000378:metabolism*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D010105:Oxygenases / Q000235:genetics* / Q000378:metabolism; D016958:Pseudomonas putida / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D064210:Secondary Metabolism; D014641:Vanillic Acid / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D000081248:Allylbenzene Derivatives; D000840:Anisoles; D001547:Benzaldehydes; D001565:Benzoates; D062425:Hydroxybenzoate Ethers; D062385:Hydroxybenzoates; D011994:Recombinant Proteins; C009333:veratric acid; C031050:isomethyleugenol; C004520:4-anisic acid; C024896:4-anisaldehyde; D010088:Oxidoreductases; D010105:Oxygenases; D014641:Vanillic Acid; C006578:anethole; C005455:piperonylic acid