title: Genomic occupancy of the transcriptional co-activators p300 and CBP.
abstract: The p300 and CBP co-activators are histone acetylases and central regulators of transcription in metazoans. The genomic occupancy of p300/CBP detected by ChIP-seq experiments can be used to identify transcriptional enhancers. However, studies in Drosophila embryos suggest that there is a preference for some transcription factors in directing p300/CBP to the genome. Although p300/CBP occupancy in general correlates with gene activation, they can also be found at silent genomic regions, which does not result in histone acetylation. Polycomb-mediated H3K27me3 is associated with repression, but does not preclude p300/CBP binding. An antagonism between H3K27ac and H3K27me3 indicates that p300/CBP may be involved in switching between repressed and active chromatin states.
authors.affiliation: The Wenner-Gren Institute, Developmental Biology, Stockholm University, Arrheniuslaboratories E3, SE-106 91 Stockholm, Sweden.
authors.forename: Per-Henrik
authors.initials: PH
authors.lastname: Holmqvist
authors.forename: Mattias
authors.initials: M
authors.lastname: Mannervik
mesh_terms: D000107:Acetylation; D000818:Animals; D050882:CREB-Binding Protein / Q000378:metabolism*; D050881:E1A-Associated p300 Protein / Q000378:metabolism*; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D020868:Gene Silencing; D016678:Genome; D006657:Histones / Q000378:metabolism; D006801:Humans; D063146:Polycomb-Group Proteins / Q000378:metabolism; D011485:Protein Binding; D012045:Regulatory Sequences, Nucleic Acid; D015533:Transcriptional Activation
chemical_list: D006657:Histones; D063146:Polycomb-Group Proteins; D050882:CREB-Binding Protein; D050881:E1A-Associated p300 Protein
keywords: CBP; ChIP-seq; Polycomb; enhancer; p300; transcription