title: Effects of androgens on cultured cells derived from canine anterior cruciate ligament.
abstract: Anterior cruciate ligament (ACL) fibroblasts obtained from beagle dogs were cultured in basal medium containing different concentrations of 1 to 10(-3) µM 5α-dihydrotestosterone (DHT) and in basal medium itself as a control. It was demonstrated that DHT promoted cell proliferation activity, expression of androgen receptor, and collagen synthesis in ACL fibroblasts as compared with control. These results suggest that sex hormones are involved in the sex difference seen in ACL rupture of dogs.
authors.affiliation: Ohno Dog and Cat Hospital, Saitama, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Ohno
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kowatari
authors.forename: Masao
authors.initials: M
authors.lastname: Owaki
authors.forename: Joji
authors.initials: J
authors.lastname: Ohta
authors.forename: Naoshi
authors.initials: N
authors.lastname: Nakajima
authors.forename: Kazuki
authors.initials: K
authors.lastname: Yoshioka
authors.forename: Ken-ichiro
authors.initials: K
authors.lastname: Mutoh
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Oyamada
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000166:cytology* / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D013196:Dihydrotestosterone / Q000494:pharmacology*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D008857:Microscopy, Interference / Q000379:methods; D011944:Receptors, Androgen / Q000378:metabolism
chemical_list: D011944:Receptors, Androgen; D013196:Dihydrotestosterone; D003094:Collagen