title: Inhibition of lung adenocarcinoma transfected with interleukin 28A recombinant adenovirus (Ad-mIFN-λ2) in vivo.
abstract: Abstract Type III interferon (IFN-λ) is a novel member of the interferon family, which preferentially promotes antiviral responses from epithelial cells and cooperates with type I IFNs in the clearance of viral infections. However, the effect of mIFN-λ2 to the LA795 lung adenocarcinoma cell is largely unknown. In this study, we transfected Ad-mIFN-λ2 vector into LA795 tumor-bearing mice to explore the effect of mIFN-λ2 on the proliferation of LA795 lung adenocarcinoma cell and on the immune response of the mice. Transfected by Ad-mIFN-λ2 vector, a significant decrease in the tumor growth, the subcutaneous tumor necrosis, cystic degeneration, and tumor apoptosis were more evident; at the same time, mIFN-λ2 protein and gene were significantly more expressed. And, flow cytometry analysis suggested that CD3(+)CD4(+), CD3(+)CD8(+), and NK (CD3(-)CD49(+)) cells were all significantly increased after transfected by Ad-mIFN-λ2. The study demonstrated that recombinant Ad-mIFN-λ2 transfection effectively inhibited the growth of LA795 lung adenocarcinoma cell, which may work through inducing apoptosis of tumor cell and regulating cell immune response.
authors.affiliation: Department of Respiratory Medicine, Affiliated People's Hospital of Jiangsu University, ZhenJiang, China.
authors.forename: Yulan
authors.initials: Y
authors.lastname: Yan
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Taofeng
authors.initials: T
authors.lastname: Zhu
authors.forename: Lixue
authors.initials: L
authors.lastname: Yuan
authors.forename: Yuxi
authors.initials: Y
authors.lastname: Ge
authors.forename: Hao
authors.initials: H
authors.lastname: Ding
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Bu
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D015153:Blotting, Western; D016207:Cytokines / Q000008:administration & dosage* / Q000235:genetics; D005434:Flow Cytometry; D015316:Genetic Therapy; D005822:Genetic Vectors; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014407:Tumor Cells, Cultured; D007378:Interleukins; D000095022:Interferon Lambda
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; C511668:interferon-lambda protein, mouse; D007378:Interleukins; D000095022:Interferon Lambda