title: Expression pattern of genes encoding nitrate and ammonium assimilating enzymes in Arabidopsis thaliana exposed to short term NaCl stress.
abstract: Key steps in nitrate nutrition and assimilation were assessed over two weeks in control and 100mM NaCl-exposed Arabidopsis thaliana (Columbia) plants. The data showed that NaCl stress lowered nitrate contents in both leaves and roots. While NaCl stress decreased ammonium contents in leaves, it increased the contents in roots at the end of treatment. A survey of transcript levels of NIA1 (At1g77760) and NIA2 (At1g37130) and nitrate reductase (NR, EC 1.6.1.6) activity in the leaves and roots suggested a major role of NIA2 rather than NIA1 in the regulation of NR by salt stress. A drop in mRNA levels for GLN2 (At5g35630) and GLN1;2 (At1g66200) by salt was associated with a similar inhibition of glutamine synthetase (GS, EC 6.3.1.2) activity in the leaves. In the roots, NaCl stress was found to enhance mRNA levels of GLN2 and cytosolic-encoding genes (GLN1;1 (At5g37600) and GLN1;2).
authors.affiliation: Unité de Recherche Nutrition et Métabolisme Azotés et Protéines de Stress 99/UR/C 09-20, Département des Sciences Biologiques, Faculté des Sciences de Tunis, 1060, Tunisia. mohamed.debouba@gmail.com
authors.forename: Mohamed
authors.initials: M
authors.lastname: Debouba
authors.forename: Houda Maaroufi
authors.initials: HM
authors.lastname: Dguimi
authors.forename: Mohamed
authors.initials: M
authors.lastname: Ghorbel
authors.forename: Houda
authors.initials: H
authors.lastname: Gouia
authors.forename: Akira
authors.initials: A
authors.lastname: Suzuki
mesh_terms: D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D050901:Nitrate Reductase / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D054712:Salinity; D012965:Sodium Chloride / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D009566:Nitrates; D010937:Plant Growth Regulators; D000644:Quaternary Ammonium Compounds; C508260:SKS6 protein, Arabidopsis; D014157:Transcription Factors; C559637:WIND1 protein, Arabidopsis; D012965:Sodium Chloride; D010088:Oxidoreductases; D050901:Nitrate Reductase