title: Recent advances in soybean transformation and their application to molecular breeding and genomic analysis.
abstract: Herbicide-resistant transgenic soybean plants hold a leading market share in the USA and other countries, but soybean has been regarded as recalcitrant to transformation for many years. The cumulative and, at times, exponential advances in genetic manipulation have made possible further choices for soybean transformation. The most widely and routinely used transformation systems are cotyledonary node-Agrobacterium-mediated transformation and somatic embryo-particle-bombardment-mediated transformation. These ready systems enable us to improve seed qualities and agronomic characteristics by transgenic approaches. In addition, with the accumulation of soybean genomic resources, convenient or promising approaches will be requisite for the determination and use of gene function in soybean. In this article, we describe recent advances in and problems of soybean transformation, and survey the current transgenic approaches for applied and basic research in Japan.
authors.affiliation: Research Faculty of Agriculture, Hokkaido University , Kita 9 Nishi 9, Kita, Sapporo, Hokkaido 060-8589, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yamada
authors.forename: Kyoko
authors.initials: K
authors.lastname: Takagi
authors.forename: Masao
authors.initials: M
authors.lastname: Ishimoto
keywords: Agrobacterium tumefaciens; Soybean [Glycine max (L.) Merrill]; particle bombardment; transformation