title: Development of transgenic CryIA(c) + GNA cotton plants via pollen tube pathway method confers resistance to Helicoverpa armigera and Aphis gossypii Glover.
abstract: Elite cotton cultivar Sumian16 was transformed with p7RPSBK-mGNA-NPTII containing Bt (CryIA(c)), Galanthus nivalis agglutinin (GNA) resistance genes and selectable marker NptII gene via the pollen tube pathway method and two fertile transgenic Bt + GNA plants were obtained in the present study. The integration and expression of the Bt and GNA genes were confirmed by molecular biology techniques and insect bioassays. Insect bioassays showed that the transformed plants were highly toxic to bollworm larvae as well as obviously retarding development of aphid populations. PCR analyses and identification of resistance to Kanamycin and bollworm showed that the resistance to bollworm for the two transgenic plants was dominantly inherited in a Mendelian manner and the two resistance genes and selectable marker co-segregated from primary transformed parents to the first self-fertilized progeny plants.
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing, PR China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Liu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhu
authors.forename: Tianzhen
authors.initials: T
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001042:Aphids / Q000502:physiology; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D001681:Biological Assay; D004731:Endotoxins / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D003368:Gossypium / Q000235:genetics* / Q000502:physiology; D006460:Hemolysin Proteins / Q000235:genetics*; D007313:Insecta / Q000502:physiology*; D007915:Lepidoptera / Q000502:physiology; D037241:Mannose-Binding Lectins / Q000235:genetics*; D010572:Pest Control, Biological / Q000379:methods*; D037121:Plant Lectins / Q000235:genetics*; D030821:Plants, Genetically Modified; D053205:Pollen Tube / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D037241:Mannose-Binding Lectins; D037121:Plant Lectins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; C063117:snowdrop lectin