title: [Preliminary study of effects of andrographolide on Staphylococcus epidermidis biofilms].
abstract: OBJECTIVE
  To provide a new therapeutic approach for Staphylococcus epidermidis biofilm-associated infections by the study of inhibitory effect of andrographolide (AG) on S. epidermidis biofilm.
  
  
  METHODS
  S. epidermidis biofilms were set up in vitro, erythromycin was acted as the positive control agent, XTT reduction assay was used to evaluate AG on the initial adhesion of S. epidermidis and bacterial metabolism within biofilm, microscope was applied to observe biofilm morphology, and Congo red assay was used to detect polysacchatide interc-ellular adhesion (PIA)formation when exposed to AG.
  
  
  RESULTS
  AG showed inhibitory effects against the initial adhesion of S. epidermidis at concentrations of 1 000,100, 10 mg x L(-1), respectively,and inhibited metabolism of biofilm bacteria at the concentration of 31.25 mg x L(-1), and exhibited significantly inhibition against the biofilm morphology at the concentration of 250 mg x L(-1), while did not display inhibition against PIA formation at the concentration of 10 mg x L(-1).
  
  
  CONCLUSIONS
  AG could remarkably inhibit biofilm formation of S. epidermidis, although it was less potent than erythromycin.
authors.affiliation: Anhui University of Traditional Chinese Medicine, Hefei 230038, China. guanyan0726@sina.com
authors.forename: Yan
authors.initials: Y
authors.lastname: Guan
authors.forename: Jiuyun
authors.initials: J
authors.lastname: Zhang
authors.forename: Changzhong
authors.initials: C
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Liu
authors.forename: Jingjin
authors.initials: J
authors.lastname: Shi
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects*; D004224:Diterpenes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004917:Erythromycin / Q000494:pharmacology; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000502:physiology
chemical_list: D004224:Diterpenes; C030419:andrographolide; D004917:Erythromycin