title: Transcription factor CREB is phosphorylated in human molar odontoblasts and cementoblasts in vivo.
abstract: A wide variety of stimuli can trigger activation of the transcription factor CREB (cAMP-responsive element binding protein), pointing toward a central role for CREB in the integration of various signaling inputs. No data are available on the expression and phosphorylation of CREB in mammalian teeth. Using immunohistochemical analysis of free-floating sections, we show here that CREB was strongly expressed and phosphorylated at Ser-133 within the nucleus of a subpopulation of adult human molar odontoblasts. Many dental pulp stromal cells and periodontal ligament fibroblasts expressed CREB and showed phosphorylation of CREB at Ser-133. In addition, cementoblasts displayed nuclear expression and phosphorylation of CREB at Ser-133. The epithelial rests of Malassez revealed strong nuclear expression of CREB, but phosphorylation at Ser-133 was variable. Our results provide the first evidence that the constitutively phosphorylated transcription factor CREB is involved in the biomineralization process of adult human molar odontoblasts and cementoblasts.
authors.affiliation: Department of Anatomy I, University of Cologne, Joseph-Stelzmann-Str. 9, 50931, Cologne, Germany. franzjosef.klinz@uni-koeln.de
authors.forename: Franz-Josef
authors.initials: FJ
authors.lastname: Klinz
authors.forename: Yüksel
authors.initials: Y
authors.lastname: Korkmaz
authors.forename: Britta
authors.initials: B
authors.lastname: Cho
authors.forename: Wolfgang H-M
authors.initials: WH
authors.lastname: Raab
authors.forename: Klaus
authors.initials: K
authors.lastname: Addicks
mesh_terms: D000293:Adolescent; D000328:Adult; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D003739:Dental Cementum / Q000378:metabolism*; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008963:Molar / Q000378:metabolism*; D009804:Odontoblasts / Q000378:metabolism*; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism; D010766:Phosphorylation; D015398:Signal Transduction; D055815:Young Adult
chemical_list: C115163:CREB1 protein, human; D017362:Cyclic AMP Response Element-Binding Protein