title: [Detection and significance of gene variation in hepatitis B virus S region].
abstract: OBJECTIVE
  To explore the variations of gene S in hepatitis B viruses of hepatitis B patients and provide experimental evidences for the mutation analysis of viral gene.
  
  
  METHODS
  The virus DNA load in hepatitis B patient donors was detected by real-time polymerase chain reaction (PCR) and gene sequence analysis. And a comparison was made with standard strain by the software DNAstar.
  
  
  RESULTS
  (1) Gene S was successfully amplified and sequenced in 15 hepatitis B patients. Three samples had I→T mutation at residue 126 in HBsAg "a" antigenic determinant. (2) Sixteen hepatitis B patients had 67 nucleotide mutations, including 14 residues in PreS1 and 6 residues in PreS2. Mutations nt 3036 T→C, nt 3039 T→G, nt 3066 C→T and L88V existed in PreS1 gene in all samples.
  
  
  CONCLUSIONS
  HBV genome is susceptible to nucleotide mutations. Some residues have geographically restricted mutations in gene S region. And understanding the significance of these mutations may help clarify the pathogenesis of hepatitis B and provide new experimental evidence for its gene diagnosis and prevention.
authors.affiliation: Department of Inspection, Penglai Municipal People's Hospital, Shandong, China. pljyz188@sina.com
authors.forename: Yong-zheng
authors.initials: YZ
authors.lastname: Jing
authors.forename: Sheng-bo
authors.initials: SB
authors.lastname: Pu
authors.forename: Yu-ping
authors.initials: YP
authors.lastname: Jia
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.forename: Ke-yi
authors.initials: KY
authors.lastname: Hu
authors.forename: Ming-gang
authors.initials: MG
authors.lastname: Qiao
authors.forename: Xue-ying
authors.initials: XY
authors.lastname: Yu
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
mesh_terms: D004252:DNA Mutational Analysis; D005814:Genes, Viral; D006509:Hepatitis B / Q000821:virology*; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D009154:Mutation; D020641:Polymorphism, Single Nucleotide
chemical_list: D006514:Hepatitis B Surface Antigens