title: Evaluation of the effect of calcium gluconate and bovine thrombin on the temporal release of transforming growth factor beta 1 and platelet-derived growth factor isoform BB from feline platelet concentrates.
abstract: BACKGROUND
  There are not reported regarding the protocols for obtaining platelet concentrates (PC) in cats for medical purposes. The objectives of this study were: 1) to describe a manual method for producing two kinds of PC in cats (PC-A and PC-B), 2) to describe the cellular population of the PC, 3) to measure and compare the effect of calcium gluconate (CG) and bovine thrombin (BT) on the temporal release of transforming growth factor beta 1 (TGF-β1) and platelet-derived growth factor type BB (PDGF-BB) at 3 and 12 hours post-activation and 4) to establish correlations between the cellular population of both PCs and the concentration of growth factors (GF). Blood samples were taken from 16 cats for complete blood count, plasma collection and PC preparation. The PC were arbitrarily divided into two fractions, specifically, PC-A (lower fraction) and PC-B (upper fraction).
  
  
  RESULTS
  The platelet counts were significantly different (P<0.05) between the PC and whole blood but not between the PC fractions. The TGF-β1 concentration efficiencies for PC-A and PC-B activated with CG were 42.86% and 46.54%, and activated with BT were 42.88% and 54.64%, respectively. The PDGF-BB concentration efficiencies for PC-A and PC-B activated with CG were 61.36% and 60.61%, and activated with BT were 65.64% and 72.12%, respectively. The temporal release of GFs showed no statistically significant difference (P>0.05) between the activating substances at the time or for any PC fraction.
  
  
  CONCLUSIONS
  Whatever the activation means, these preparations of cat PC provide significant concentrations of platelets and GFs for possible clinical or experimental use.
authors.affiliation: Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Caldas, Colombia. raul.silva@ucaldas.edu.co
authors.forename: Raul F
authors.initials: RF
authors.lastname: Silva
authors.forename: María E
authors.initials: ME
authors.lastname: Alvarez
authors.forename: Diana L
authors.initials: DL
authors.lastname: Ríos
authors.forename: Catalina
authors.initials: C
authors.lastname: López
authors.forename: Jorge U
authors.initials: JU
authors.lastname: Carmona
authors.forename: Cleuza Mf
authors.initials: CM
authors.lastname: Rezende
mesh_terms: D000818:Animals; D000077214:Becaplermin; D001792:Blood Platelets / Q000378:metabolism*; D001800:Blood Specimen Collection; D002125:Calcium Gluconate / Q000494:pharmacology*; D002415:Cats; D002417:Cattle; D005260:Female; D008297:Male; D020574:Proto-Oncogene Proteins c-sis / Q000235:genetics / Q000378:metabolism*; D013917:Thrombin / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D020574:Proto-Oncogene Proteins c-sis; D053773:Transforming Growth Factor beta1; D000077214:Becaplermin; D013917:Thrombin; D002125:Calcium Gluconate