title: [Nuclear factor κB and IKB expression and calcium deposition of atherosclerotic plaques in apolipoprotein E and low density lipoprotein receptor knockout mice].
abstract: OBJECTIVE
  To observe the histopathological features, nuclear factor-κB (NFκB) and IKB expressions as well as calcium deposition of atherosclerosis plaques (AS) in apolipoprotein E (ApoE) and low density lipoprotein receptor (LDLR) knockout mice (ApoE(-/-), LDLR(-/-)fed high-fat diet.
  
  
  METHODS
  Eight C57BL/6J mice fed with normal diet were used as control, 32 ApoE(-/-) mice and LDLR(-/-) mice were divided into normal diet and high-fat diet groups (n = 8 each). After 4 months, aorta was collected for morphologic (HE, Oil Red O, Von Kossa) and immunohistochemistry (nuclear factor-κB, IKB, macrophage surface molecule-3, α-smooth action protein) analysis.
  
  
  RESULTS
  Degree of AS in ApoE(-/-) and LDLR(-/-) mice fed with high-fat diet were significantly severer than those fed with normal diet and AS was more significant in ApoE(-/-) mice than in LDLR(-/-) mice. NFκB and IKB expressions in high-fat diet group were significantly higher than the normal diet group (P < 0.05). Double-labeling of NFκB revealed dominant expression in smooth muscle cells. Calcium deposition was significantly more in ApoE(-/-) mice fed with high-fat diet than mice fed with normal diet (P < 0.05) and was similar in LDLR(-/-) mice fed with high and normal diet (P > 0.05).
  
  
  CONCLUSIONS
  High-fat diet contributes to the formation of AS plagues in ApoE(-/-) and LDLR(-/-) mice joined by upregulated NFκB and IKB expressions and calcium deposition.
authors.affiliation: Department of Medical Laboratory, Central South University, Changsha, China.
authors.forename: Feng-zhen
authors.initials: FZ
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Wen-wen
authors.initials: WW
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Pu-yuan
authors.initials: PY
authors.lastname: Wen
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.forename: Jian-gang
authors.initials: JG
authors.lastname: Wang
authors.forename: Xing-xing
authors.initials: XX
authors.lastname: Zheng
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D005260:Female; D020672:I-kappa B Proteins / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016328:NF-kappa B / Q000378:metabolism*; D058226:Plaque, Atherosclerotic / Q000378:metabolism* / Q000473:pathology; D011973:Receptors, LDL / Q000235:genetics / Q000378:metabolism*
chemical_list: D001057:Apolipoproteins E; D020672:I-kappa B Proteins; D016328:NF-kappa B; D011973:Receptors, LDL; D002118:Calcium