title: VimA-dependent modulation of the secretome in Porphyromonas gingivalis.
abstract: The VimA protein of Porphyromonas gingivalis is a multifunctional protein involved in cell surface biogenesis. To further determine if its acetyl coenzyme A (acetyl-CoA) transfer and putative sorting functions can affect the secretome, its role in peptidoglycan biogenesis and effects on the extracellular proteins of P. gingivalis FLL92, a vimA-defective mutant, were evaluated. There were structural and compositional differences in the peptidoglycan of P. gingivalis FLL92 compared with the wild-type strain. Sixty-eight proteins were present only in the extracellular fraction of FLL92. Fifteen proteins present in the extracellular fraction of the parent strain were missing in the vimA-defective mutant. These proteins had protein sorting characteristics that included a C-terminal motif with a common consensus Gly-Gly-CTERM pattern and a polar tail consisting of aromatic amino acid residues. These observations suggest that the VimA protein is likely involved in peptidoglycan synthesis, and corroborates our previous report, which suggests a role in protein sorting.
authors.affiliation: Division of Microbiology & Molecular Genetics, School of Medicine, Loma Linda University, Loma Linda, CA 92350, USA.
authors.forename: D
authors.initials: D
authors.lastname: Osbourne
authors.forename: A Wilson
authors.initials: AW
authors.lastname: Aruni
authors.forename: Y
authors.initials: Y
authors.lastname: Dou
authors.forename: C
authors.initials: C
authors.lastname: Perry
authors.forename: D S
authors.initials: DS
authors.lastname: Boskovic
authors.forename: F
authors.initials: F
authors.lastname: Roy
authors.forename: H M
authors.initials: HM
authors.lastname: Fletcher
mesh_terms: D000105:Acetyl Coenzyme A / Q000378:metabolism; D020816:Amino Acid Motifs / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D020868:Gene Silencing; D005998:Glycine / Q000032:analysis; D006384:Hemagglutination; D006461:Hemolysis; D006801:Humans; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D009154:Mutation / Q000235:genetics; D009842:Oligopeptides / Q000032:analysis; D010457:Peptidoglycan / Q000096:biosynthesis* / Q000235:genetics; D010641:Phenotype; D016966:Porphyromonas gingivalis / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000502:physiology; D021381:Protein Transport / Q000235:genetics; D059748:Proteolysis; D020543:Proteome / Q000235:genetics; D053719:Tandem Mass Spectrometry
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D009842:Oligopeptides; D010457:Peptidoglycan; D020543:Proteome; D000105:Acetyl Coenzyme A; D005998:Glycine