title: Overlapping genetic codes for overlapping frameshifted genes in Testudines, and Lepidochelys olivacea as special case.
abstract: Mitochondrial genes code for additional proteins after +2 frameshifts by reassigning stops to code for amino acids, which defines overlapping genetic codes for overlapping genes. Turtles recode stops UAR → Trp and AGR → Lys (AGR → Gly in the marine Olive Ridley turtle, Lepidochelys olivacea). In Lepidochelys the +2 frameshifted mitochondrial Cytb gene lacks stops, open reading frames from other genes code for unknown proteins, and for regular mitochondrial proteins after frameshifts according to the overlapping genetic code. Lepidochelys' inversion between proteins coded by regular and overlapping genetic codes substantiates the existence of overlap coding. ND4 differs among Lepidochelys mitochondrial genomes: it is regular in DQ486893; in NC_011516, the open reading frame codes for another protein, the regular ND4 protein is coded by the frameshifted sequence reassigning stops as in other turtles. These systematic patterns are incompatible with Genbank/sequencing errors and DNA decay. Random mixing of synonymous codons, conserving main frame coding properties, shows optimization of natural sequences for overlap coding; Ka/Ks analyses show high positive (directional) selection on overlapping genes. Tests based on circular genetic codes confirm programmed frameshifts in ND3 and ND4l genes, and predicted frameshift sites for overlap coding in Lepidochelys. Chelonian mitochondria adapt for overlapping gene expression: cloverleaf formation by antisense tRNAs with predicted anticodons matching stops coevolves with overlap coding; antisense tRNAs with predicted expanded anticodons (frameshift suppressor tRNAs) associate with frameshift-coding in ND3 and ND4l, a potential regulation of frameshifted overlap coding. Anaeroby perhaps switched between regular and overlap coding genes in Lepidochelys.
authors.affiliation: Center for Ecological and Evolutionary Synthesis, Department of Biological Sciences, University of Oslo, Blindern, N-0316 Oslo, Norway. podarcissicula@gmail.com
authors.forename: Hervé
authors.initials: H
authors.lastname: Seligmann
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics*; D018965:Frameshifting, Ribosomal / Q000235:genetics*; D005796:Genes / Q000235:genetics*; D005815:Genetic Code / Q000235:genetics*; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D013045:Species Specificity; D014426:Turtles / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial; D011506:Proteins