title: Counting chromosomes in intact eggs.
abstract: Chromosomal spreads are an established method to assess ploidy in different cell types. However, many traditional chromosome-spreading techniques require dissolution of the cell and can only be used to assess hyperploidy because of potential chromosome loss inherent in the procedure. Here we describe a method to evaluate chromosome numbers in intact eggs so that both hyperploidy and hypoploidy can be accurately detected.
authors.affiliation: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
authors.forename: Teresa
authors.initials: T
authors.lastname: Chiang
authors.forename: Michael A
authors.initials: MA
authors.lastname: Lampson
mesh_terms: D000818:Animals; D033481:Chromosomes, Mammalian / Q000378:metabolism*; D003584:Cytological Techniques / Q000379:methods*; D006801:Humans; D051379:Mice; D010063:Ovum / Q000166:cytology*