title: Matrix-assisted laser desorption/ionisation time of flight spectrometry for the fast screening of oxosteroids using aromatic hydrated hydrazines as versatile probes.
abstract: To make the use of MALDI-based mass spectrometry feasible for the fast analysis of oxosteroids, three new aromatic probes have been designed to be used simultaneously as derivatisation agents and MALDI matrices. This concept brings a number of benefits: the sample handling is reduced, the workflow is less time consuming allowing high throughput and the interferences caused by the MALDI matrix are avoided. Identification was successfully attained for all oxosteroids used in this study. As proof-of-concept, the identification of oxosteroids in urine was performed to evaluate the robustness of the new methodology. The oxosteroids 17-α-methyltestosterone, nandrolone, boldenone, 17-α-Trenbolone, fluoxymesterolone, mesterolone and bolasterone were identified in human urine at the minimum concentration level recommended by the world anti-doping agency, 2 ng/mL.
authors.affiliation: REQUIMTE/CQFB, Grupo Bioscope, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Monte de Caparica, Portugal.
authors.forename: Marco
authors.initials: M
authors.lastname: Galesio
authors.forename: Cristina
authors.initials: C
authors.lastname: Núñez
authors.forename: Mario S
authors.initials: MS
authors.lastname: Diniz
authors.forename: Richard
authors.initials: R
authors.lastname: Welter
authors.forename: Carlos
authors.initials: C
authors.lastname: Lodeiro
authors.forename: José
authors.initials: J
authors.lastname: Luis Capelo
mesh_terms: D006801:Humans; D006834:Hydrazines / Q000138:chemical synthesis / Q000737:chemistry*; D007664:Ketosteroids / Q000032:analysis* / Q000737:chemistry* / Q000652:urine; D008958:Models, Molecular; D008968:Molecular Conformation; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors
chemical_list: D006834:Hydrazines; D007664:Ketosteroids; D015335:Molecular Probes