title: Fluorescence behavioral imaging (FBI) tracks identity in heterogeneous groups of Drosophila.
abstract: Distinguishing subpopulations in group behavioral experiments can reveal the impact of differences in genetic, pharmacological and life-histories on social interactions and decision-making. Here we describe Fluorescence Behavioral Imaging (FBI), a toolkit that uses transgenic fluorescence to discriminate subpopulations, imaging hardware that simultaneously records behavior and fluorescence expression, and open-source software for automated, high-accuracy determination of genetic identity. Using FBI, we measure courtship partner choice in genetically mixed groups of Drosophila.
authors.affiliation: Center for Integrative Genomics, University of Lausanne, Lausanne, Switzerland. pavan.ramdya@epfl.ch
authors.forename: Pavan
authors.initials: P
authors.lastname: Ramdya
authors.forename: Thomas
authors.initials: T
authors.lastname: Schaffter
authors.forename: Dario
authors.initials: D
authors.lastname: Floreano
authors.forename: Richard
authors.initials: R
authors.lastname: Benton
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D003380:Courtship; D004330:Drosophila / Q000235:genetics / Q000378:metabolism*; D005260:Female; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D008954:Models, Biological; D061848:Optical Imaging; D014741:Video Recording
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins