title: GAL promoter-driven heterologous gene expression in Saccharomyces cerevisiae Δ strain at anaerobic alcoholic fermentation.
abstract: The removal of Gal80 protein by gene disruption turned into efficient GAL promoter-driven heterologous gene expression under anaerobic alcoholic fermentation of Saccharomyces cerevisiae. Using lipase B from Candida antarctica as a reporter, the relative strength of GAL10 promoter (P(GAL10) ) in Δgal80 mutant that does not require galactose as an inducer was compared to those of ADH1, PDC1, and PGK promoters, which have been known to work well anaerobically in actively fermenting yeast cells under high glucose concentration. P(GAL10)  in the Δgal80 mutant showed 0.8-fold (ADH1), fourfold (PDC1), and 50-fold (PGK) in promoter strength.
authors.affiliation: Biotechnology Process Engineering Center, KRIBB, Yuseong-gu, Daejeon, Korea.
authors.forename: Jungoh
authors.initials: J
authors.lastname: Ahn
authors.forename: Kyung-Min
authors.initials: KM
authors.lastname: Park
authors.forename: Hongweon
authors.initials: H
authors.lastname: Lee
authors.forename: Yeo-Jin
authors.initials: YJ
authors.lastname: Son
authors.forename: Eui-Sung
authors.initials: ES
authors.lastname: Choi
mesh_terms: D000693:Anaerobiosis; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005818:Genetic Engineering / Q000379:methods*; D005947:Glucose / Q000378:metabolism; D008049:Lipase / Q000235:genetics* / Q000378:metabolism; D030781:Organisms, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics*; D019076:Transgenes
chemical_list: D005656:Fungal Proteins; C518355:GAL10 protein, S cerevisiae; C055002:GAL80 protein, S cerevisiae; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D000431:Ethanol; D008049:Lipase; C431349:lipase B, Candida antarctica; D005947:Glucose; D005690:Galactose