title: Effect of methanolic and water extract of Leucobryum bowringii Mitt. on growth, migration and invasion of MCF 7 human breast cancer cells in vitro.
abstract: Inhibitory effects of methanol and water extract of L. bowringii. on the adhesion, migration, invasion and matrix metalloproteinase (MMP) activities of MCF 7 human breast cancer cell line are reported. Cells were cultured with 10, 25, 50 microg/mL methanolic or water extract of L. bowringii. Culture medium containing 0.1% DMSO was used as a solvent control. Ultra structural analysis by electron microscopy revealed typical features of apoptosis. A remarkable dose-response parallelism was observed between methanolic extract with growth, migration and invasion of breast cancer cells. Fractionation of methanolic extract by RP-HPLC revealed a pool of phenolic acids. Hoechst 33342 staining assay reveals massive chromatin condensation and subsequent cleavage of structural components of nucleus. The results indicate that methanol extracts inhibit the growth of human breast cancer cells partially through the inhibition of metallo proteinases MMP-2 and MMP-9 activities. Methanolic extract has more anti-metastatic effects in cell based assay than water extract. Clinical application of L. bowringii extract as a bioactive chemopreventive compound may be helpful in limiting breast carcinoma invasion and metastasis.
authors.affiliation: Plant Biochemistry and Molecular Biology Laboratory, Department of Botany, University College, Thiruvananthapuram 695 034, India.
authors.forename: G S
authors.initials: GS
authors.lastname: Manoj
authors.forename: T R Santhosh
authors.initials: TR
authors.lastname: Kumar
authors.forename: Saneesh
authors.initials: S
authors.lastname: Varghese
authors.forename: K
authors.initials: K
authors.lastname: Murugan
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy; D019068:Bryopsida / Q000737:chemistry*; D002465:Cell Movement / Q000187:drug effects*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D061986:MCF-7 Cells / Q000187:drug effects; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000517:prevention & control; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology
chemical_list: D010936:Plant Extracts; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9