title: Antagonistic properties of seagrass associated Streptomyces sp. RAUACT-1: a source for anthraquinone rich compound.
abstract: OBJECTIVE
  To identify the antibacterial potential of seagrass (Syringodium isoetifolium) associate microbes against bacterial pathogens.
  
  
  METHODS
  Eumeration of microbial associates were analyzed with leaf and root samples of Syringodium isoetifolium. MIC and MBC were calculated for bacterial pathogens with microbial associates. Phylogenetic and GC-MS analysis were calculated for Actinomycetes sp. (Act01) which was the most potent.
  
  
  RESULTS
  Of the isolated microbial associates phosphatase producing bacterial isolates were identified as maximum [(261.78±35.09) CFU×10(4)/g] counts in root sample. Of the selected microbial isolates Actinomycete sp (Act01) showed broad spectrum of antibacterial activity against antibiotic resistant and fish bacterial pathogens. Phylogenetic analysis of Act01 showed maximum identities (99%) with the Streptomyces sp. (GU5500072). The 16s rDNA secondary structure of Act01 showed the free energy values as -366.3 kkal/mol. The GC-MS analysis Act01 showed maximum retention value with 23.742 RT and the corresponding chemical class was identified as 1, 4-dihydroxy-2-(3-hydroxybutyl)-9, 10-anthraquinone 9, 10-anthrac.
  
  
  CONCLUSIONS
  In conclusion, Streptomyces sp. (GU045544.1) from Syringodium isoetifolium could be used as potential antibacterial agent.
authors.affiliation: Department of Oceanography and Coastal Area Studies, Alagappa University, Tamilnadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Ravikumar
authors.forename: M
authors.initials: M
authors.lastname: Gnanadesigan
authors.forename: A
authors.initials: A
authors.lastname: Saravanan
authors.forename: N
authors.initials: N
authors.lastname: Monisha
authors.forename: V
authors.initials: V
authors.lastname: Brindha
authors.forename: S
authors.initials: S
authors.lastname: Muthumari
mesh_terms: D035961:Alismatales / Q000382:microbiology*; D000880:Anthraquinones / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D000898:Antibiosis; D058491:Bacterial Load; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008401:Gas Chromatography-Mass Spectrometry; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S