title: The putative α/β-hydrolases of Dietzia cinnamea P4 strain as potential enzymes for biocatalytic applications.
abstract: The draft genome of the soil actinomycete Dietzia cinnamea P4 reveals a versatile group of α/β-hydrolase fold enzymes. Phylogenetic and comparative sequence analyses were used to classify the α/β-hydrolases of strain P4 into six different groups: (i) lipases, (ii) esterases, (iii) epoxide hydrolases, (iv) haloacid dehalogenases, (v) C-C breaking enzymes and (vi) serine peptidases. The high number of lipases/esterases (41) and epoxide hydrolase enzymes (14) present in the relatively small (3.6 Mb) P4 genome is unusual; it is likely to be linked to the survival of strain P4 in its natural environment. Strain P4 is thus equipped with a large number of genes which would appear to confer survivability in harsh hot tropical soil. As such, this highly resilient soil bacterial strain provides an interesting genome for enzyme mining for applications in the field of biotransformations of polymeric compounds.
authors.affiliation: Laboratório de Genética Microbiana, Departamento de Microbiologia Geral, Instituto de Microbiologia Prof. Paulo de Góes, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Bloco I, Ilha do Fundão, Rio de Janeiro, RJ, CEP 21941.590, Brazil.
authors.forename: Luciano
authors.initials: L
authors.lastname: Procópio
authors.forename: Andrew
authors.initials: A
authors.lastname: Macrae
authors.forename: Jan Dirk
authors.initials: JD
authors.lastname: van Elsas
authors.forename: Lucy
authors.initials: L
authors.lastname: Seldin
mesh_terms: D000192:Actinomycetales / Q000201:enzymology* / Q000235:genetics; D001408:Bacillus anthracis / Q000235:genetics* / Q000302:isolation & purification; D001709:Biotechnology / Q000379:methods*; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics*; D016680:Genome, Bacterial; D006867:Hydrolases / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D004269:DNA, Bacterial; D006867:Hydrolases