title: An improved mass spectrometric method for identification and quantification of phenolic compounds in apple fruits.
abstract: Thirty-nine phenolic compounds were analysed using ultra high performance liquid chromatography (UHPLC) coupled with diode array and accurate mass spectrometry detection using electrospray ionisation (DAD/ESI-am-MS). Instrumental parameters such as scan speed, resolution, and mass accuracy were optimised to establish accurate mass measurements. The method was fully validated in terms of model deviation (r(2)>0.9990), range (typically 10-3500 ngg(-1)), intra/inter-day precision (<6% and <8%, respectively) and accuracy (typically 100 ± 10%). The mass accuracy of each selected phenolic compound was below 1.5 ppm. The results confirmed that the UHPLC-DAD/ESI-am-MS method developed here was convenient and reliable for the determination of phenolic compounds in apple extracts.
authors.affiliation: Flemish Institute for Technological Research (VITO), Environmental Analysis and Technology, Boeretang 200, 2400 Mol, Belgium; Institute domien.depaepe@ilvo.vlaanderen.be
authors.forename: D
authors.initials: D
authors.lastname: De Paepe
authors.forename: K
authors.initials: K
authors.lastname: Servaes
authors.forename: B
authors.initials: B
authors.lastname: Noten
authors.forename: L
authors.initials: L
authors.lastname: Diels
authors.forename: M
authors.initials: M
authors.lastname: De Loose
authors.forename: B
authors.initials: B
authors.lastname: Van Droogenbroeck
authors.forename: S
authors.initials: S
authors.lastname: Voorspoels
mesh_terms: D005638:Fruit / Q000737:chemistry*; D027845:Malus / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D010636:Phenols; D010936:Plant Extracts