title: Molecular genotyping of Mycobacterium bovis isolated from cattle tissues in the North West Region of Cameroon.
abstract: An epidemiological study was carried out to determine the Mycobacterium bovis strains causing bovine tuberculosis (TB) in cattle in North West Cameroon. Suspected TB lesions from slaughtered cattle were cultured on Lowenstein-Jensen and Middlebrook 7 H9 media to isolate mycobacteria agents for molecular genotyping using deletion analysis and spoligotyping. PCR-based genomic deletion typing showed that 54 of 103 tubercle bacilli isolated from cattle tissue were M. bovis strains and the African 1 clonal complex was widespread in affected cattle. Spoligotyping analysis revealed a closely related group of five M. bovis strains. SB0953, the dominant spoligotype pattern, and four new patterns identified as SB2161, SB2162, SB2663 and SB2664 according to the                    www.Mbovis.org                                     international spoligotype database were identified. These spoligotypes were similar to other M. bovis strains recovered from bordering regions and other parts of Africa. The findings provided useful facts on the zoonotic risks of bovine TB and overwhelming evidence of the significance of M. bovis infection to human TB in the North West Region of Cameroon. The study revealed that bovine TB was widespread in cattle destined for human consumption and also has important implications for the control of TB in animals and humans in Cameroon.
authors.affiliation: Department of Animal Sciences, University of Dschang, Dschang, Cameroon. awahndukum@yahoo.co.uk
authors.forename: Julius
authors.initials: J
authors.lastname: Awah-Ndukum
authors.forename: Ayuba Caleb
authors.initials: AC
authors.lastname: Kudi
authors.forename: Graham
authors.initials: G
authors.lastname: Bradley
authors.forename: Noel H
authors.initials: NH
authors.lastname: Smith
authors.forename: Irene
authors.initials: I
authors.lastname: Ane-Anyangwe
authors.forename: Fidelis
authors.initials: F
authors.lastname: Cho-Ngwa
authors.forename: Vicent Pryde Kehdinga
authors.initials: VP
authors.lastname: Titanji
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000662:veterinary; D002163:Cameroon / Q000453:epidemiology; D002417:Cattle; D058889:Molecular Typing / Q000662:veterinary; D060885:Multiplex Polymerase Chain Reaction / Q000662:veterinary; D009163:Mycobacterium bovis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D014380:Tuberculosis, Bovine / Q000453:epidemiology* / Q000382:microbiology / Q000473:pathology