title: Are gastric cancer resection margin proteomic profiles more similar to those from controls or tumors?
abstract: A strategy for treating cancer is to surgically remove the tumor together with a portion of apparently healthy tissue surrounding it, the so-called "resection margin", to minimize recurrence. Here, we investigate whether the proteomic profiles from biopsies of gastric cancer resection margins are indeed more similar to those from healthy tissue than from cancer biopsies. To this end, we analyzed biopsies using an offline MudPIT shotgun proteomic approach and performed label-free quantitation through a distributed normalized spectral abundance factor approach adapted for extracted ion chromatograms (XICs). A multidimensional scaling analysis revealed that each of those tissue-types is very distinct from each other. The resection margin presented several proteins previously correlated with cancer, but also other overexpressed proteins that may be related to tumor nourishment and metastasis, such as collagen alpha-1, ceruloplasmin, calpastatin, and E-cadherin. We argue that the resection margin plays a key role in Paget's "soil to seed" hypothesis, that is, that cancer cells require a special microenvironment to nourish and that understanding it could ultimately lead to more effective treatments.
authors.affiliation: Departamento de Química, Universidade Federal do Amazonas, Amazonas, Brazil.
authors.forename: Priscila F
authors.initials: PF
authors.lastname: Aquino
authors.forename: Juliana S G
authors.initials: JS
authors.lastname: Fischer
authors.forename: Ana G C
authors.initials: AG
authors.lastname: Neves-Ferreira
authors.forename: Jonas
authors.initials: J
authors.lastname: Perales
authors.forename: Gilberto B
authors.initials: GB
authors.lastname: Domont
authors.forename: Gabriel D T
authors.initials: GD
authors.lastname: Araujo
authors.forename: Valmir C
authors.initials: VC
authors.lastname: Barbosa
authors.forename: Jucilana
authors.initials: J
authors.lastname: Viana
authors.forename: Sidney R S
authors.initials: SR
authors.lastname: Chalub
authors.forename: Antonia Q
authors.initials: AQ
authors.lastname: Lima de Souza
authors.forename: Maria G C
authors.initials: MG
authors.lastname: Carvalho
authors.forename: Afonso D
authors.initials: AD
authors.lastname: Leão de Souza
authors.forename: Paulo C
authors.initials: PC
authors.lastname: Carvalho
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis* / Q000378:metabolism; D001706:Biopsy; D015820:Cadherins / Q000378:metabolism; D016022:Case-Control Studies; D002570:Ceruloplasmin / Q000378:metabolism; D002852:Chromatography, Ion Exchange / Q000379:methods; D024161:Collagen Type XI / Q000378:metabolism; D030562:Databases, Protein; D005260:Female; D006801:Humans; D008297:Male; D009362:Neoplasm Metastasis / Q000175:diagnosis; D009363:Neoplasm Proteins / Q000378:metabolism; D011379:Prognosis; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D011706:Pyloric Antrum / Q000378:metabolism / Q000473:pathology; D012984:Software; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C574182:COL11A1 protein, human; D015820:Cadherins; D024161:Collagen Type XI; D009363:Neoplasm Proteins; D020543:Proteome; C114751:TPD52 protein, human; D002570:Ceruloplasmin