title: Evaluation of immunohistochemical markers of lymphatic and blood vessels in canine mammary tumours.
abstract: Canine mammary tumours (CMTs) are the most common neoplasms in intact female dogs. Bitches with spontaneously arising CMTs represent a promising animal model for human breast cancer research. The aim of the present study was to develop an immunohistochemical protocol for the identification of blood and lymphatic vessels in CMTs. Antibodies specific for human lymphatic vessels (prox-1, lyve-1, podoplanin and D2-40) and blood vessels (von Willebrand factor [vWf], CD31 and CD34) were utilized. Serial sections of 18 samples (eight samples of normal canine mammary tissue, five benign and five malignant CMTs) were examined. Antibodies specific for podoplanin, D2-40 and CD34 showed no immunoreactivity with canine tissue. Prox-1 and CD31 were determined to be the most suitable markers for lymphatic and blood vessels, respectively.
authors.affiliation: Applied Veterinary Morphology, Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium. nathalie.sleeckx@ua.ac.be
authors.forename: N
authors.initials: N
authors.lastname: Sleeckx
authors.forename: L
authors.initials: L
authors.lastname: Van Brantegem
authors.forename: E
authors.initials: E
authors.lastname: Fransen
authors.forename: G
authors.initials: G
authors.lastname: Van den Eynden
authors.forename: C
authors.initials: C
authors.lastname: Casteleyn
authors.forename: E
authors.initials: E
authors.lastname: Veldhuis Kroeze
authors.forename: C
authors.initials: C
authors.lastname: Van Ginneken
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001808:Blood Vessels / Q000378:metabolism*; D004283:Dog Diseases / Q000378:metabolism* / Q000473:pathology; D004285:Dogs; D005260:Female; D018398:Homeodomain Proteins / Q000378:metabolism; D042601:Lymphatic Vessels / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000378:metabolism* / Q000473:pathology; D015674:Mammary Neoplasms, Animal / Q000378:metabolism* / Q000473:pathology; D008562:Membrane Glycoproteins / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D025521:Tumor Suppressor Proteins / Q000378:metabolism; D033921:Vesicular Transport Proteins / Q000378:metabolism
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D015415:Biomarkers; D018398:Homeodomain Proteins; D008562:Membrane Glycoproteins; D025521:Tumor Suppressor Proteins; D033921:Vesicular Transport Proteins; C500987:monoclonal antibody D2-40