title: Sol-gel-derived bioactive glass containing SiO2-MgO-CaO-P2O5 as an antibacterial scaffold.
abstract: Bioactive glass (BG) composites with a base of SiO2-Na2O-CaO-P2O5 are biocompatible biomaterials. The assessment of their abilities for medical applications has interested researchers. We produced a BG-containing SiO2-MgO-CaO-P2O5 by the sol-gel method. To determine the antibacterial effects, we analyzed the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) properties of this product on three microorganisms, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, known causative agents for biofilm formation on implant surfaces. In addition, we performed the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay to study the cytotoxic effects of our composite on animal cells. Our results demonstrated that our BG product inhibited the growth of bacteria in a concentration-dependent manner without any cytotoxic effects. Therefore, our BG product can be utilized as an appropriate implant for treating bone and tooth defects.
authors.affiliation: Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Abbas Ali Imani
authors.initials: AA
authors.lastname: Fooladi
authors.forename: Hamideh Mahmoodzadeh
authors.initials: HM
authors.lastname: Hosseini
authors.forename: Forough
authors.initials: F
authors.lastname: Hafezi
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Hosseinnejad
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Nourani
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004926:Escherichia coli / Q000187:drug effects; D005898:Glass / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D053758:Nanoparticles / Q000648:ultrastructure; D044367:Phase Transition / Q000187:drug effects*; D011208:Powders; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013194:Staining and Labeling; D013211:Staphylococcus aureus / Q000187:drug effects; D054457:Tissue Scaffolds / Q000737:chemistry*; D014343:Trypan Blue / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D011208:Powders; C525209:SiO2-CaO-MgO-P2O5 bioglass; D014343:Trypan Blue