title: [Expression of cytokines of interleukin-6 family in gingival fibroblasts by Toll-like receptor-2].
abstract: OBJECTIVE
  To investigate whether signaling through Toll-like receptor-2 (TLR-2) can affect the expression of some cytokines in human gingival fibroblasts.
  
  
  METHODS
  The gingival fibroblasts were isolated and cultured in vivo, divided into blank control group, lipopolysaccharide (LPS) from Porphyromonas gingivalis (Pg) group and Escherichia coli (Ec) group. mRNA expression levels were measured by real-time polymerase chain reaction (PCR). The protein expression levels were detected by the enzyme linked immunosorbent assay (ELISA). The data was statistically analyzed by SPSS16.0 software package.
  
  
  RESULTS
  LPS from Pg could stimulate the expression of interleukin (IL)-6 and leukemia inhibitory factor (LIF) mRNA and protein, which reached the peak (5.87 ± 0.83) at 10 h, and the expression level increased with the increase of the Pg concentration. IL-11 or oncostatin-M (OSM) mRNA expression was not affected by LPS. After treated with Pg for 48 h, the protein expression of IL-6 and LIF was up-regulated, (962 ± 57) ng/L and (47 ± 18) ng/L respectively.
  
  
  CONCLUSIONS
  Signaling through TLR-2 controls the expression of cytokines of IL-6 family in human gingival fibroblasts.
authors.affiliation: Department of Stomatology, People's Hospital of Tianjin, 300121 Tianjin, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xin
authors.forename: Yan-hua
authors.initials: YH
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
mesh_terms: D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D002648:Child; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D005881:Gingiva / Q000166:cytology; D006801:Humans; D017370:Interleukin-11 / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D053605:Leukemia Inhibitory Factor / Q000235:genetics / Q000378:metabolism*; D055666:Lipopeptides / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D053683:Oncostatin M / Q000235:genetics / Q000378:metabolism; D016966:Porphyromonas gingivalis / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D051195:Toll-Like Receptor 2 / Q000819:agonists*; D055815:Young Adult
chemical_list: C508611:IL11 protein, human; C508600:IL6 protein, human; D017370:Interleukin-11; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; D055666:Lipopeptides; D008070:Lipopolysaccharides; C508351:OSM protein, human; C508075:Pam(3)CSK(4) peptide; C560312:Pam2CSK4 lipopeptide; D012333:RNA, Messenger; C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2; D053683:Oncostatin M