title: First molecular cloning and gene expression analysis of a teleost CD200 (OX-2) glycoprotein from rock bream, Oplegnathus fasciatus.
abstract: CD200 plays an important role in delivering an immunoregulatory signal to the immune system through interaction with its receptor. However, CD200 has not been characterized and its function in teleosts is unknown. In this study, the rock bream (Oplegnathus fasciatus) CD200 gene (RbCD200) was cloned and its expression profile was analyzed after infection with Edwardsiella tarda, Streptococcus iniae or red seabream iridovirus (RSIV). The coding region of RbCD200 cDNA was 855 bp, encoding 284 amino acid residues. The gene consisted of two extracellular Ig-like domains and a transmembrane domain. RbCD200 was highly expressed in the brain, erythrocytes, intestine and stomach of healthy rock bream. In the spleen, RbCD200 gene expression was down-regulated until 48 h after E. tarda exposure, except at 12 h RbCD200 gene expression was down-regulated then up-regulated at 12 h and 24 h after infection with S. iniae and RSIV, respectively. In the whole kidney, the RbCD200 gene was down-regulated in response to infection with E. tarda and S. iniae. However, RSIV infection increased RbCD200 gene expression in whole kidney until 48 h. These results suggest that RbCD200 is differentially expressed in the spleen and whole kidney after infection with different pathogens.
authors.affiliation: Institute of Marine Industry, College of Marine Science, Gyeongsang National University, Tongyeong, Gyeongnam 650-160, Republic of Korea.
authors.forename: Seong Don
authors.initials: SD
authors.lastname: Hwang
authors.forename: Ju-Won
authors.initials: JW
authors.lastname: Kim
authors.forename: Mu-Chan
authors.initials: MC
authors.lastname: Kim
authors.forename: Do-Hyung
authors.initials: DH
authors.lastname: Kim
authors.forename: Chan-Il
authors.initials: CI
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D003001:Cloning, Molecular; D004266:DNA Virus Infections / Q000276:immunology / Q000662:veterinary; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D020609:Edwardsiella tarda / Q000502:physiology; D004756:Enterobacteriaceae Infections / Q000276:immunology / Q000662:veterinary; D020224:Expressed Sequence Tags; D029941:Fish Proteins / Q000235:genetics / Q000276:immunology*; D015698:Genomic Library; D007113:Immunity, Innate; D007497:Iridoviridae / Q000502:physiology; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010473:Perciformes / Q000235:genetics / Q000276:immunology*; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D013290:Streptococcal Infections / Q000276:immunology / Q000662:veterinary; D013291:Streptococcus / Q000502:physiology
chemical_list: D015703:Antigens, CD; D018076:DNA, Complementary; D029941:Fish Proteins; D012333:RNA, Messenger; C042739:antigens, CD200