title: Insulinotropic action of Citrullus colocynthis seed extracts in rat pancreatic islets.
abstract: The present study aimed to investigate the direct in vitro effects of several distinct Citrullus colocynthis seed extracts on glucose-stimulated insulin release from pancreatic islets isolated from rats. Six extracts were tested, a crude aqueous, defatted aqueous, ethyl acetate, H2O-methanol and n-butanol extract and an extract containing a major component (fraction A) identified by gel chromatography in the ethyl acetate, n-butanol and H2O-methanol extracts. Under selected experimental conditions, the majority of extracts exhibited a positive insulinotropic action, at least when tested in the presence of 8.3 mM D-glucose. The concentration-response correlation observed with distinct extracts revealed the participation of distinct chemical compounds, including compounds with an inhibitory insulinotropic potential, in the modulation of the insulin secretory response to D-glucose. The results of the present study are relevant for further investigations which aim to identify compounds exhibiting positive insulinotropic actions. These agents may be suitable for the treatment of human diabetic subjects.
authors.affiliation: Laboratory of Antibiotic and Antifungal Physico-Chemistry, Synthesis and Biological Activity, Faculty of Sciences, University Abou Bekr Belakaïd, Tlemcen, Algeria.
authors.forename: Nabila
authors.initials: N
authors.lastname: Benariba
authors.forename: Rabeh
authors.initials: R
authors.lastname: Djaziri
authors.forename: Emeline
authors.initials: E
authors.lastname: Hupkens
authors.forename: Karim
authors.initials: K
authors.lastname: Louchami
authors.forename: Willy J
authors.initials: WJ
authors.lastname: Malaisse
authors.forename: Abdullah
authors.initials: A
authors.lastname: Sener
mesh_terms: D000818:Animals; D029773:Citrullus / Q000737:chemistry*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D045022:Lactate Dehydrogenases / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D012639:Seeds / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
chemical_list: D007328:Insulin; D010936:Plant Extracts; D012997:Solvents; D045022:Lactate Dehydrogenases