title: [Dopamine as a possible substance for oncotherapy and for quantitative evaluation of cytosolic G-actin].
abstract: Viability, histology and ultrastructure of normal cells and cells of different degrees of malignancy after interaction with dopamine as well as the ability of these cells and isolated G-actin in model experiments to stain by Falck technique were studied. It is shown that dopamine, virtually having no effect on the viability of the "normal" non-tumorigenic transformed cells, noticeably reduces cell viability of slightly tumorigenic cells, causes a significant reduction in viability of attachable cancerous cells and a very significant decrease in cell viability of cancerous cells growing in suspension. The intensity of fluorescence of the cytosole in cells treated with dopamine, has been very high and varied in different cultures, and that of isolated actin directly depended on its concentration. Common to all cell morphological feature of damage from the action of dopamine and the putative substrate of fluorescence was actodopamine filaments network strands (identified on the structure and size), which appears in the cytosole loci, where they were absent in control. The data show that dopamine can be used as an oncotherapeutic remedy and diagnostic tool interacting with G-actin as a cellular target.
authors.forename: E Iu
authors.initials: EIu
authors.lastname: Parnyshkova
authors.forename: E N
authors.initials: EN
authors.lastname: Bezgina
authors.forename: L I
authors.initials: LI
authors.lastname: Kazakova
authors.forename: I M
authors.initials: IM
authors.lastname: Vikhliantsev
authors.forename: N P
authors.initials: NP
authors.lastname: Tiras
authors.forename: L L
authors.initials: LL
authors.lastname: Pavlik
authors.forename: D A
authors.initials: DA
authors.lastname: Moshkov
mesh_terms: D000199:Actins / Q000032:analysis* / Q000648:ultrastructure; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D004298:Dopamine / Q000494:pharmacology*; D005347:Fibroblasts / Q000737:chemistry / Q000187:drug effects* / Q000648:ultrastructure; D018698:Glutamic Acid / Q000494:pharmacology; D006801:Humans; D051379:Mice; D009000:Monocytes / Q000737:chemistry / Q000187:drug effects* / Q000648:ultrastructure; D009928:Organ Specificity; D013050:Spectrometry, Fluorescence
chemical_list: D000199:Actins; D000970:Antineoplastic Agents; D018698:Glutamic Acid; D004298:Dopamine