title: Induction of trans-resveratrol and extracellular pathogenesis-related proteins in elicited suspension cultured cells of Vitis vinifera cv Monastrell.
abstract: Suspension-cultured cells of Vitis vinifera cv Monastrell were used to investigate the effects of methyljasmonate, ethylene and salicylic acid separately or in combination with cyclodextrins on both trans-resveratrol production and the induction of defense responses. The results showed that the addition of methyljasmonate or ethylene to suspension-cultured cells jointly treated with cyclodextrins and salicylic acid provoked a decrease of trans-resveratrol levels suggesting that salicylic acid has a negative and antagonistic effect with methyljasmonate or ethylene on trans-resveratrol production. Likewise, the exogenous application of these compounds induced the accumulation of pathogenesis-related proteins. Analysis of the extracellular proteome showed the presence of amino acid sequences homologous to an specific β-1,3-glucanase, class III peroxidases and a β-1,4-mannanase, which suggests that these signal molecules could play a role in mediating defense-related gene product expression in V. vinifera cv Monastrell. Apart from these inducible proteins, other proteins were found in both the control and elicited cell cultures of V. vinifera. These included class IV chitinase, polygalacturonase inhibitor protein and reticuline oxidase-like protein, suggesting that their expression is constitutive being involved in the modification of the cell wall architecture during cell culture growth and in the prevention of pathogen attack.
authors.affiliation: Department of Plant Biology, University of Murcia, Campus Universitario de Espinardo, E-30100 Murcia, Spain.
authors.forename: Sarai
authors.initials: S
authors.lastname: Belchí-Navarro
authors.forename: Lorena
authors.initials: L
authors.lastname: Almagro
authors.forename: Ana Belén
authors.initials: AB
authors.lastname: Sabater-Jara
authors.forename: Francisco
authors.initials: F
authors.lastname: Fernández-Pérez
authors.forename: Roque
authors.initials: R
authors.lastname: Bru
authors.forename: Maria Angeles
authors.initials: MA
authors.lastname: Pedreño
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D003505:Cyclodextrins / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology; D060467:Disease Resistance / Q000187:drug effects*; D005030:Ethylenes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D054883:Oxylipins / Q000494:pharmacology; D010935:Plant Diseases / Q000276:immunology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000378:metabolism*; D000077185:Resveratrol; D020156:Salicylic Acid / Q000494:pharmacology; D015398:Signal Transduction; D013267:Stilbenes / Q000378:metabolism*; D027843:Vitis / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*
chemical_list: D000085:Acetates; D000890:Anti-Infective Agents; D003505:Cyclodextrins; D003517:Cyclopentanes; D005030:Ethylenes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D013267:Stilbenes; C053376:pathogenesis-related proteins, plant; C072239:methyl jasmonate; C036216:ethylene; D020156:Salicylic Acid; D000077185:Resveratrol