title: [Effect of lipopolysaccharides extracted from Porphyromonas endodontalis on the expression of p38 and ERK1/2 in osteoblast].
abstract: OBJECTIVE
  To investigate the effect of lipopolysaccharides (LPS) extracted from Porphyromonas endodontalis(P.e) on the expression of p38 and ERK1/2 in osteoblast.
  
  
  METHODS
  MC3T3-E1 cells were stimulated with 10 μg/mL P.e-LPS for 0,5,15,30,60,180 min. The phosphorylation of p38 and ERK1/2 was measured by Western blot. Statistical analysis was performed using one- way ANOVA and Dunnett t test with SPSS11.0 software package.
  
  
  RESULTS
  10 μg/mL LPS could significantly activate p38 MAPK. The peak of phosphorylated p38 was detected at 5 to 30 min(P<0.01) and returned to baseline within 60 min; the level of phosphorylated ERK1/2 increased after the stimulation of LPS for 5 min and reached maximum at 15 min (P<0.01) and declined after 30 min.
  
  
  CONCLUSIONS
  P.e-LPS can induce the expression of p38 and ERK1/2 in osteoblast MC3T3-E1, which indicates that P.e-LPS may play an important role in osteoblast through p38 and ERK1/2.
authors.affiliation: Department of Endodontics, School of Stomatology, China Medical University. Shenyang 110002, Liaoning Province, China. 281476736@qq.com
authors.forename: You
authors.initials: Y
authors.lastname: Lv
authors.forename: Ge
authors.initials: G
authors.lastname: Jia
authors.forename: Li-hong
authors.initials: LH
authors.lastname: Qiu
authors.forename: Mu-rong
authors.initials: MR
authors.lastname: Bao
authors.forename: Ya-qiong
authors.initials: YQ
authors.lastname: Yu
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
mesh_terms: D006801:Humans; D008070:Lipopolysaccharides; D010006:Osteoblasts; D010766:Phosphorylation; D045202:Porphyromonas endodontalis
chemical_list: D008070:Lipopolysaccharides