title: Preparation and characterization of phospholipid-conjugated indocyanine green as a near-infrared probe.
abstract: We have rationally designed and synthesized a novel near-infrared (NIR) photoactivating probe, designated by iDOPE, in which an indocyanine green (ICG) fluorophore is covalently conjugated with a phospholipid moiety, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), to incorporate into liposome bilayers. NIR irradiation showed that iDOPE retained the optical and fluorescence properties of ICG and demonstrated photoactivator characteristics: fluorescence emission at around 820 nm in a solvent, singlet oxygen production, and concentration-dependent heat generation. Additionally, iDOPE was incorporated into liposome bilayers and maintained stable liposomally formulated iDOPE (LP-iDOPE) over 1week under physiological conditions. We also observed the tumor-specific biodistribution of LP-iDOPE of in vivo xenografts. These findings suggest that LP-iDOPE might be a promising tool for NIR optical imaging, photodynamic therapy, and photothermal therapy.
authors.affiliation: Department of Bioinformatics, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan. a.suganami@restaff.chiba-u.jp
authors.forename: Akiko
authors.initials: A
authors.lastname: Suganami
authors.forename: Taro
authors.initials: T
authors.lastname: Toyota
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Okazaki
authors.forename: Kengo
authors.initials: K
authors.lastname: Saito
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Akutsu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawahira
authors.forename: Akira
authors.initials: A
authors.lastname: Aoki
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Muraki
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Madono
authors.forename: Hideki
authors.initials: H
authors.lastname: Hayashi
authors.forename: Hisahiro
authors.initials: H
authors.lastname: Matsubara
authors.forename: Takashige
authors.initials: T
authors.lastname: Omatsu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shirasawa
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tamura
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry* / Q000493:pharmacokinetics; D006801:Humans; D007208:Indocyanine Green / Q000737:chemistry*; D007259:Infrared Rays; D008051:Lipid Bilayers / Q000737:chemistry / Q000493:pharmacokinetics*; D008081:Liposomes / Q000737:chemistry; D051379:Mice; D008958:Models, Molecular; D015394:Molecular Structure; D009374:Neoplasms, Experimental / Q000188:drug therapy*; D010714:Phosphatidylethanolamines / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008081:Liposomes; D010714:Phosphatidylethanolamines; C020888:1,2-dielaidoylphosphatidylethanolamine; D007208:Indocyanine Green