title: Novel activity-based probes for broad-spectrum profiling of retaining β-exoglucosidases in situ and in vivo.
abstract: A high-end label: Cyclophellitol aziridine-type activity-based probes allow for ultra-sensitive visualization of mammalian β-glucosidases (GBA1, GBA2, GBA3, and LPH) as well as several non-mammalian β-glucosidases (see picture). These probes offer new ways to study β-exoglucosidases, and configurational isomers of the cyclophellitol aziridine core may give activity-based probes targeting other retaining glycosidase families.
authors.affiliation: Department of Medicinal Biochemistry, Academic Medical Center, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands.
authors.forename: Wouter W
authors.initials: WW
authors.lastname: Kallemeijn
authors.forename: Kah-Yee
authors.initials: KY
authors.lastname: Li
authors.forename: Martin D
authors.initials: MD
authors.lastname: Witte
authors.forename: André R A
authors.initials: AR
authors.lastname: Marques
authors.forename: Jan
authors.initials: J
authors.lastname: Aten
authors.forename: Saskia
authors.initials: S
authors.lastname: Scheij
authors.forename: Jianbing
authors.initials: J
authors.lastname: Jiang
authors.forename: Lianne I
authors.initials: LI
authors.lastname: Willems
authors.forename: Tineke M
authors.initials: TM
authors.lastname: Voorn-Brouwer
authors.forename: Cindy P A A
authors.initials: CP
authors.lastname: van Roomen
authors.forename: Roelof
authors.initials: R
authors.lastname: Ottenhoff
authors.forename: Rolf G
authors.initials: RG
authors.lastname: Boot
authors.forename: Hans
authors.initials: H
authors.lastname: van den Elst
authors.forename: Marthe T C
authors.initials: MT
authors.lastname: Walvoort
authors.forename: Bogdan I
authors.initials: BI
authors.lastname: Florea
authors.forename: Jeroen D C
authors.initials: JD
authors.lastname: Codée
authors.forename: Gijsbert A
authors.initials: GA
authors.lastname: van der Marel
authors.forename: Johannes M F G
authors.initials: JM
authors.lastname: Aerts
authors.forename: Herman S
authors.initials: HS
authors.lastname: Overkleeft
mesh_terms: D000818:Animals; D001388:Aziridines / Q000737:chemistry; D001921:Brain / Q000201:enzymology; D044602:Cellulases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D003511:Cyclohexanols / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D007536:Isomerism; D051379:Mice; D040901:Proteomics; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics
chemical_list: D001388:Aziridines; D003511:Cyclohexanols; D005456:Fluorescent Dyes; D011994:Recombinant Proteins; C063285:cyclophellitol; C033132:aziridine; D044602:Cellulases