title: Role of leukotriene B₄ receptor signaling in human preadipocyte differentiation.
abstract: We investigated the role of leukotriene B(4) (LTB(4))-leukotriene receptor (BLT) signaling in preadipocyte differentiation into mature adipocytes. Blockade of BLT signaling by treatment with lipoxygenase inhibitors, a BLT antagonist, and small interfering RNAs for BLTs in human and mouse preadipocytes isolated from adipose tissues showed acceleration of differentiation into mature adipocytes. DNA microarray analysis revealed regulation of transforming growth factor, beta-induced 68 kDa (TGFBI) expression through the BLT signaling pathway during adipocyte differentiation. Knockdown of TGFBI also showed acceleration of preadipocyte differentiation. The LTB(4)-BLT signaling pathway may negatively regulate preadipocyte differentiation via induction of TGFBI expression as a rate-limiting system to control adipocyte differentiation.
authors.affiliation: Department of Pharmacology, Graduate School of Dentistry, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Kae
authors.initials: K
authors.lastname: Hirata
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Katayama
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nakajima
authors.forename: Kenji
authors.initials: K
authors.lastname: Takada
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kamisaki
authors.forename: Koichiro
authors.initials: K
authors.lastname: Wada
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D000818:Animals; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis / Q000235:genetics; D055785:Gene Knockdown Techniques; D006801:Humans; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D051379:Mice; D034741:RNA, Small Interfering / Q000235:genetics; D018102:Receptors, Leukotriene B4 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics; D000099222:betaIG-H3 Protein
chemical_list: D016326:Extracellular Matrix Proteins; D016859:Lipoxygenase Inhibitors; D034741:RNA, Small Interfering; D018102:Receptors, Leukotriene B4; D016212:Transforming Growth Factor beta; D000099222:betaIG-H3 Protein; C513240:LTB4R protein, human; C485878:LTB4R2 protein, human