title: Improvement of gene targeting in Aspergillus nidulans with excess non-homologous fragments.
abstract: Efficiency of gene targeting was increased more than 10-fold when an excess molar ratio of non-homologous fragments was added in the transformation of Aspergillus nidulans. In the targeted transformants, integration of such fragments into the host chromosome was a rare event. Hence, our approach proved practical in terms of producing successful targeting events without disturbing the host chromosomes.
authors.affiliation: Graduate School of Science and Technology, Shinshu University, Matsumoto, Nagano 390-8621, Japan.
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Matsuhira
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Itoh
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics*; D015825:Chromosomes, Fungal / Q000235:genetics; D018390:Gene Targeting / Q000379:methods*; D012689:Sequence Homology, Nucleic Acid; D014170:Transformation, Genetic