title: Oral immunotherapy for pollen allergy using T-cell epitope-containing egg white derived from genetically manipulated chickens.
abstract: Peptide immunotherapy using T-cell epitopes is expected to be an effective treatment for allergic diseases such as Japanese cedar (Cryptomeria japonica; Cj) pollinosis. To develop a treatment for pollen allergy by inducing oral tolerance, we generated genetically manipulated (GM) chickens by retroviral gene transduction, to produce a fusion protein of chicken egg white lysozyme and a peptide derived from seven dominant human T-cell epitopes of Japanese cedar pollen allergens (cLys-7crp). The transgene sequence was detected in all chickens transduced with the retroviral vector. Transduction efficiency in blood cells correlated to transgene expression. Western blot analysis revealed that cLys-7crp was expressed in the egg white of GM hens. Mice induced to develop allergic rhinitis by Cj pollinosis were fed with cLys-7crp-containing egg white produced by GM chickens. Total and Cj allergen (Cry j 1)-specific IgE levels were significantly decreased in allergic mice fed with cLys-7crp-containing egg white compared with allergic mice fed with normal egg white. These results suggest that oral administration of T-cell epitope-containing egg white derived from GM chickens is effective for the induction of immune tolerance as an allergy therapy.
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Kyushu University, Fukuoka, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kawabe
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Hayashida
authors.forename: Kensaku
authors.initials: K
authors.lastname: Numata
authors.forename: Shota
authors.initials: S
authors.lastname: Harada
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Hayashida
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
authors.forename: Masamichi
authors.initials: M
authors.lastname: Kamihira
mesh_terms: D000284:Administration, Oral; D000485:Allergens / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D015153:Blotting, Western; D002642:Chick Embryo; D002645:Chickens; D032485:Cryptomeria / Q000276:immunology; D004529:Egg White; D004797:Enzyme-Linked Immunosorbent Assay; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007167:Immunotherapy / Q000379:methods*; D051379:Mice; D008969:Molecular Sequence Data; D009113:Muramidase / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D041681:NIH 3T3 Cells; D011058:Pollen / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology* / Q000628:therapy; D016896:Treatment Outcome
chemical_list: D000485:Allergens; D018984:Epitopes, T-Lymphocyte; D011993:Recombinant Fusion Proteins; D007073:Immunoglobulin E; D009113:Muramidase