title: [Activity of phenylalanine-ammonia-lyase in callus cultures of sugar beat infected by Acholeplasma].
abstract: The effect of Acholeplasma laidlawii var. granulum 118 on activity of phenylalanine-ammonia-lyase (PAL) in callus cultures of sugar beat was researched. The optimal conditions of enzyme reaction were: using the L-phenilalanine as a substrate, pH 8.4-8.8, the temperature optimum 38-40 degrees C. It was established that at the infecting of sugar beat callus culture by phytopathogenic mollicute the PAL activity was temporarily increased and reached its maximum after 2 h of infecting. Then it gradually decreased and in 24 h reached its initial level. An increase of PAL activity of plant is considered as protective reaction in response to the action of pathogen.
authors.forename: L P
authors.initials: LP
authors.lastname: Panchenko
authors.forename: E S
authors.initials: ES
authors.lastname: Korobkova
mesh_terms: D000128:Acholeplasma laidlawii / Q000254:growth & development*; D027461:Beta vulgaris / Q000201:enzymology* / Q000276:immunology / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D010649:Phenylalanine / Q000378:metabolism*; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism*; D057865:Plant Immunity / Q000502:physiology; D010940:Plant Proteins / Q000378:metabolism*; D013237:Stereoisomerism; D013379:Substrate Specificity; D013696:Temperature; D046509:Tissue Culture Techniques
chemical_list: D010940:Plant Proteins; D010649:Phenylalanine; D010650:Phenylalanine Ammonia-Lyase