title: Merging of image data in electron crystallography.
abstract: Electron crystallography of membrane proteins uses cryo-transmission electron microscopy to record images and diffraction patterns of frozen-hydrated 2D crystals. Each two-dimensional (2D) crystal is only imaged once, at one specific tilt angle, and the recorded images can be automatically processed with the 2dx/MRC software package. Processed image data from non-tilted and tilted 2D crystals then need to be merged into a 3D reconstruction of the membrane protein structure. We here describe the process of the 3D merging, using the 2dx software system.
authors.affiliation: C-CINA, Biozentrum, University Basel, Basel, Switzerland.
authors.forename: Marcel
authors.initials: M
authors.lastname: Arheit
authors.forename: Daniel
authors.initials: D
authors.lastname: Castaño-Diéz
authors.forename: Raphaël
authors.initials: R
authors.lastname: Thierry
authors.forename: Priyanka
authors.initials: P
authors.lastname: Abeyrathne
authors.forename: Bryant R
authors.initials: BR
authors.lastname: Gipson
authors.forename: Henning
authors.initials: H
authors.lastname: Stahlberg
mesh_terms: D020285:Cryoelectron Microscopy / Q000379:methods; D003461:Crystallography / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry / Q000648:ultrastructure; D012984:Software
chemical_list: D008565:Membrane Proteins