title: Structure of glycerol-3-phosphate dehydrogenase (GPD1) from Saccharomyces cerevisiae at 2.45 Å resolution.
abstract: The interconversion of glycerol 3-phosphate and dihydroxyacetone phosphate by glycerol-3-phosphate dehydrogenases provides a link between carbohydrate and lipid metabolism and provides Saccharomyces cerevisiae with protection against osmotic and anoxic stress. The first structure of a glycerol-3-phosphate dehydrogenase from S. cerevisiae, GPD1, is reported at 2.45 Å resolution. The asymmetric unit contains two monomers, each of which is organized with N- and C-terminal domains. The N-terminal domain contains a classic Rossmann fold with the (β-α-β-α-β)2 motif typical of many NAD+-dependent enzymes, while the C-terminal domain is mainly α-helical. Structural and phylogenetic comparisons reveal four main structure types among the five families of glycerol-3-phosphate and glycerol-1-phosphate dehydrogenases and reveal that the Clostridium acetobutylican protein with PDB code 3ce9 is a glycerol-1-phosphate dehydrogenase.
authors.affiliation: Institute for Research in Biomedicine, Parc Científic, Baldiri Reixac 10, 08028 Barcelona, Spain.
authors.forename: David Aparicio
authors.initials: DA
authors.lastname: Alarcon
authors.forename: Munmun
authors.initials: M
authors.lastname: Nandi
authors.forename: Xavi
authors.initials: X
authors.lastname: Carpena
authors.forename: Ignacio
authors.initials: I
authors.lastname: Fita
authors.forename: Peter C
authors.initials: PC
authors.lastname: Loewen
mesh_terms: D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+) / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D058977:Molecular Sequence Annotation; D010802:Phylogeny; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics; D040681:Structural Homology, Protein
chemical_list: D029701:Saccharomyces cerevisiae Proteins; C568531:GPD1 protein, S cerevisiae; D050536:Glycerol-3-Phosphate Dehydrogenase (NAD+)