title: Genotypic diversity, antibiotic resistance and bacteriocin production of enterococci isolated from rhizospheres.
abstract: This study aimed to identify and to characterize rhizospheric-derived enterococci. The results showed the prevalence of Enterococcus faecium species (97%) vs. Enterococcus durans (3%). Susceptibility testing for antibiotics showed a low percentage of resistance to erythromycin (3.2%) and tetracycline (11.2%), and intermediate resistance to vancomycin (6.5%). Nevertheless, a high proportion of bacteriocin production was recorded. Furthermore, PCR detection of antibiotic resistance and bacteriocin production-encoding genes was investigated. Pulsed-field gel electrophoresis typing (PFGE) showed a great variability of enterococci in the rhizosphere. Moreover, mutilocus-sequence-typing analysis (MLST) revealed the identification of three new sequence types (STs), which were registered as ST613, ST614 and ST615.
authors.affiliation: Laboratoire de Microorganismes et Biomolécules actives, Département de Biologie, Campus Universitaire, 2092 Tunis, Tunisia.
authors.forename: Naouel
authors.initials: N
authors.lastname: Klibi
authors.forename: Naouel
authors.initials: N
authors.lastname: Ben Slimen
authors.forename: Imen
authors.initials: I
authors.lastname: Fhoula
authors.forename: Maria
authors.initials: M
authors.lastname: López
authors.forename: Karim
authors.initials: K
authors.lastname: Ben Slama
authors.forename: Daniele
authors.initials: D
authors.lastname: Daffonchio
authors.forename: Abdellatif
authors.initials: A
authors.lastname: Boudabous
authors.forename: Carmen
authors.initials: C
authors.lastname: Torres
authors.forename: Hadda
authors.initials: H
authors.lastname: Ouzari
mesh_terms: D001430:Bacteriocins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D044822:Biodiversity; D002939:Ciprofloxacin / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016984:Enterococcus faecium / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D004917:Erythromycin / Q000494:pharmacology; D014644:Genetic Variation; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D018517:Plant Roots / Q000382:microbiology*; D058441:Rhizosphere; D013752:Tetracycline / Q000494:pharmacology; D013753:Tetracycline Resistance / Q000235:genetics; D014640:Vancomycin / Q000494:pharmacology; D020713:Vancomycin Resistance / Q000235:genetics
chemical_list: D001430:Bacteriocins; D002939:Ciprofloxacin; D004917:Erythromycin; D014640:Vancomycin; D013752:Tetracycline