title: Development of chloroplast microsatellite markers for phylogenetic analysis in Brassicaceae.
abstract: By employing in silico tools, we devised new chloroplast microsatellite primers for inferring phylogenetic relationships within Brassicaceae. Microsatellite repeats were scanned in 12 chloroplast genomes of Brassicaceae, regions flanking these repeats were aligned and 19 universal primers were designed. Fifteen of these primer pairs are predicted to yield polymorphic amplicons, that are more or less evenly distributed throughout the chloroplast genomes. Finally, using PCR, we have validated three primer pairs on a limited 'test set' of plants, different from those used in computational analysis.
authors.affiliation: Plant Biotechnology Division, Indian Institute of Integrative Medicine, Council of Scientific and Industrial Research, Canal Road, Jammu, 180 001, India.
authors.forename: Praveen
authors.initials: P
authors.lastname: Awasthi
authors.forename: Irshad
authors.initials: I
authors.lastname: Ahmad
authors.forename: Sumit G
authors.initials: SG
authors.lastname: Gandhi
authors.forename: Yashbir S
authors.initials: YS
authors.lastname: Bedi
mesh_terms: D019607:Brassicaceae / Q000145:classification / Q000235:genetics*; D002736:Chloroplasts / Q000235:genetics*; D003198:Computer Simulation; D018744:DNA, Plant / Q000032:analysis*; D030541:Databases, Genetic; D005819:Genetic Markers; D018895:Microsatellite Repeats; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers