title: Effects of depletion of glutathione on abscisic acid- and methyl jasmonate-induced stomatal closure in Arabidopsis thaliana.
abstract: Glutathione (GSH) is involved in abscisic acid (ABA)- and methyl jasmonate (MeJA)-induced stomatal closure in Arabidopsis thaliana. In this study, we examined the effects of GSH-decreasing chemicals, p-nitrobenzyl chloride (PNBC), iodomethane (IDM), and ethacrynic acid (EA), on ABA- and MeJA-induced stomatal closure in Arabidopsis. Treatments with PNBC, IDM, and EA decreased GSH contents in guard cells. Depletion of GSH by PNBC and IDM enhanced ABA- and MeJA-induced stomatal closure and inhibition of light-induced stomatal opening by ABA, whereas EA did not enhance either ABA- and MeJA-induced stomatal closure or inhibition of light-induced stomatal opening by ABA. Depletion of GSH did not significantly increase the production of the reactive oxygen species (ROS), cytosolic alkalization, or cytosolic Ca(2+) oscillation induced by ABA and MeJA. These results indicate that depletion of GSH enhances ABA- and MeJA-induced stomatal closure without affecting ROS production, cytosolic alkalization, or cytosolic Ca(2+) oscillation in guard cells of Arabidopsis.
authors.affiliation: Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan.
authors.forename: Nasima
authors.initials: N
authors.lastname: Akter
authors.forename: Muhammad Abdus
authors.initials: MA
authors.lastname: Sobahan
authors.forename: Misugi
authors.initials: M
authors.lastname: Uraji
authors.forename: Wenxiu
authors.initials: W
authors.lastname: Ye
authors.forename: Mohammad Anowar
authors.initials: MA
authors.lastname: Hossain
authors.forename: Izumi C
authors.initials: IC
authors.lastname: Mori
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Murata
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D000085:Acetates / Q000494:pharmacology*; D017360:Arabidopsis / Q000033:anatomy & histology* / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects; D020013:Calcium Signaling / Q000187:drug effects / Q000528:radiation effects; D003517:Cyclopentanes / Q000494:pharmacology*; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D004976:Ethacrynic Acid / Q000378:metabolism / Q000494:pharmacology; D005978:Glutathione / Q000172:deficiency* / Q000378:metabolism; D006847:Hydrocarbons, Iodinated / Q000378:metabolism / Q000494:pharmacology; D008027:Light; D009578:Nitrobenzenes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D054883:Oxylipins / Q000494:pharmacology*; D054046:Plant Stomata / Q000033:anatomy & histology* / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D006847:Hydrocarbons, Iodinated; D009578:Nitrobenzenes; D054883:Oxylipins; D017382:Reactive Oxygen Species; D000040:Abscisic Acid; C072239:methyl jasmonate; C014055:methyl iodide; C036077:nitrobenzene; D005978:Glutathione; D004976:Ethacrynic Acid