title: Influence of Ginkgo biloba extract on the proliferation, apoptosis of ACC-2 cell and Survivin gene expression in adenoid cystic carcinoma of lacrimal gland.
abstract: OBJECTIVE
  To explore the influence of extract of Ginkgo biloba (EGB) on the proliferation, apoptosis of ACC-2 cell and Survivin gene expression in adenoid cystic carcinoma (ACC) of lacrimal gland.
  
  
  METHODS
  ACC-2 cell in human with ACC of lacrimal gland was in vitro cultured. MTT method was used for cell proliferation detection. Annexin V/PI double-staining flow cytometer was used to detect cell apoptosis and cell cycle. Survivin gene expression was analyzed by RT-PCR and Western blotting.
  
  
  RESULTS
  EGB had inhibitory effect on the proliferation of ACC-2 cell with significant dose-effect relationship, and there was statistical difference when compared with the control group (P<0.01). The inhibitory concentration 50 % (IC(50)) is 88 mg/L. The flow cytometer test indicated that EGB can gradually increase ACC-2 cell in G(0)-G(1) stage and decrease it in G(2)-M and S stage. With the increase of dose, the apoptosis rate of ACC-2 cell was obviously increased (P<0.05 or P<0.01). EGB had certain inhibitory effect on Survivin gene expression of ACC-2 cell, and Survivin gene expression was decreased with the increasing of the EGB concentration (P<0.01).
  
  
  CONCLUSIONS
  EGB can effectively inhibit Survivin gene expression of ACC-2 cell in human with ACC of lacrimal gland, induce the apoptosis of ACC-2 cell and inhibit tumor cell proliferation.
authors.affiliation: Department of Ophthalmology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Li-Xiao
authors.initials: LX
authors.lastname: Zhou
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D003528:Carcinoma, Adenoid Cystic / Q000473:pathology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects*; D020441:Ginkgo biloba / Q000737:chemistry*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000096:biosynthesis; D007765:Lacrimal Apparatus / Q000473:pathology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D013194:Staining and Labeling / Q000379:methods; D000077022:Survivin; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D010936:Plant Extracts; D000077022:Survivin; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue