title: Generation of human induced pluripotent stem cells from urine samples.
abstract: Human induced pluripotent stem cells (iPSCs) have been generated with varied efficiencies from multiple tissues. Yet, acquiring donor cells is, in most instances, an invasive procedure that requires laborious isolation. Here we present a detailed protocol for generating human iPSCs from exfoliated renal epithelial cells present in urine. This method is advantageous in many circumstances, as the isolation of urinary cells is simple (30 ml of urine are sufficient), cost-effective and universal (can be applied to any age, gender and race). Moreover, the entire procedure is reasonably quick--around 2 weeks for the urinary cell culture and 3-4 weeks for the reprogramming--and the yield of iPSC colonies is generally high--up to 4% using retroviral delivery of exogenous factors. Urinary iPSCs (UiPSCs) also show excellent differentiation potential, and thus represent a good choice for producing pluripotent cells from normal individuals or patients with genetic diseases, including those affecting the kidney.
authors.affiliation: Key Laboratory of Regenerative Biology, Chinese Academy of Sciences, and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Guangzhou, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhou
authors.forename: Christina
authors.initials: C
authors.lastname: Benda
authors.forename: Sarah
authors.initials: S
authors.lastname: Dunzinger
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Huang
authors.forename: Jenny Cy
authors.initials: JC
authors.lastname: Ho
authors.forename: Jiayin
authors.initials: J
authors.lastname: Yang
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Ya
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhuang
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Li
authors.forename: Xichen
authors.initials: X
authors.lastname: Bao
authors.forename: Hung-Fat
authors.initials: HF
authors.lastname: Tse
authors.forename: Johannes
authors.initials: J
authors.lastname: Grillari
authors.forename: Regina
authors.initials: R
authors.lastname: Grillari-Voglauer
authors.forename: Duanqing
authors.initials: D
authors.lastname: Pei
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Esteban
mesh_terms: D018929:Cell Culture Techniques; D002469:Cell Separation / Q000379:methods*; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D007668:Kidney / Q000166:cytology*; D014556:Urine / Q000166:cytology*