title: PTEN/MAPK pathways play a key role in platelet-activating factor-induced experimental pulmonary tumor metastasis.
abstract: In this study, we investigated the role of PTEN (phosphatase and tensin homolog deleted on chromosome 10) in a platelet-activating factor (PAF)-induced experimental pulmonary tumor metastasis model. An adenovirus carrying PTEN cDNA (Ad-PTEN) reversed PAF-induced increase in phosphorylation of AKT as well as pulmonary metastasis of B16F10. PAF-induced pulmonary metastasis was inhibited by MAPK inhibitors, but not by PI3K inhibitor. Ad-PTEN abrogated PAF-induced phosphorylation of MAPKs. These data indicate PTEN/MAPK pathways play a key role in PAF-induced tumor metastasis.
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Han-A
authors.initials: HA
authors.lastname: Kim
authors.forename: Kyoung-Jin
authors.initials: KJ
authors.lastname: Kim
authors.forename: Kook Heon
authors.initials: KH
authors.lastname: Seo
authors.forename: Hern-Ku
authors.initials: HK
authors.lastname: Lee
authors.forename: Suhn-Young
authors.initials: SY
authors.lastname: Im
mesh_terms: D000730:Androstadienes / Q000627:therapeutic use; D000818:Animals; D000873:Anthracenes / Q000627:therapeutic use; D002070:Butadienes / Q000627:therapeutic use; D002460:Cell Line; D006801:Humans; D007093:Imidazoles / Q000627:therapeutic use; D008175:Lung Neoplasms / Q000201:enzymology* / Q000556:secondary*; D020935:MAP Kinase Signaling System; D008546:Melanoma, Experimental / Q000556:secondary*; D051379:Mice; D008810:Mice, Inbred C57BL; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors; D009570:Nitriles / Q000627:therapeutic use; D051059:PTEN Phosphohydrolase / Q000378:metabolism*; D000081082:Phosphoinositide-3 Kinase Inhibitors; D010766:Phosphorylation; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011725:Pyridines / Q000627:therapeutic use; D000077191:Wortmannin
chemical_list: D000730:Androstadienes; D000873:Anthracenes; D002070:Butadienes; D007093:Imidazoles; D009570:Nitriles; D000081082:Phosphoinositide-3 Kinase Inhibitors; D010972:Platelet Activating Factor; D011725:Pyridines; C113580:U 0126; C432165:pyrazolanthrone; D051057:Proto-Oncogene Proteins c-akt; D020928:Mitogen-Activated Protein Kinases; D051059:PTEN Phosphohydrolase; C487757:Pten protein, mouse; C090942:4-(4-fluorophenyl)-2-(4-hydroxyphenyl)-5-(4-pyridyl)imidazole; D000077191:Wortmannin