title: Role of homologous recombination in adaptive diversification of extraintestinal Escherichia coli.
abstract: The contribution of homologous exchange (recombination) of core genes in the adaptive evolution of bacterial pathogens is not well understood. To investigate this, we analyzed fully assembled genomes of two Escherichia coli strains from sequence type 131 (ST131), a clonal group that is both the leading cause of extraintestinal E. coli infections and the main source of fluoroquinolone-resistant E. coli. Although the sequences of each of the seven multilocus sequence typing genes were identical in the two ST131 isolates, the strains diverged from one another by homologous recombination that affected at least 9% of core genes. This was on a par with the contribution to genomic diversity of horizontal gene transfer and point gene mutation. The genomic positions of recombinant and mobile genetic regions were partially linked, suggesting their concurrent occurrence. One of the genes affected by homologous recombination was fimH, which encodes mannose-specific type 1 fimbrial adhesin, resulting in functionally distinct copies of the gene in ST131 strains. One strain, a uropathogenic isolate, had a pathoadaptive variant of fimH that was acquired by homologous replacement into the commensal strain background. Close examination of FimH structure and function in additional ST131 isolates revealed that recombination led to acquisition of several functionally distinct variants that, upon homologous exchange, were targeted by a variety of pathoadaptive mutations under strong positive selection. Different recombinant fimH strains also showed a strong clonal association with ST131 isolates that had distinct fluoroquinolone resistance profiles. Thus, homologous recombination of core genes plays a significant role in adaptive diversification of bacterial pathogens, especially at the level of clonally related groups of isolates.
authors.affiliation: Department of Microbiology, University of Washington, Seattle, WA, USA.
authors.forename: Sandip
authors.initials: S
authors.lastname: Paul
authors.forename: Elena V
authors.initials: EV
authors.lastname: Linardopoulou
authors.forename: Mariya
authors.initials: M
authors.lastname: Billig
authors.forename: Veronika
authors.initials: V
authors.lastname: Tchesnokova
authors.forename: Lance B
authors.initials: LB
authors.lastname: Price
authors.forename: James R
authors.initials: JR
authors.lastname: Johnson
authors.forename: Sujay
authors.initials: S
authors.lastname: Chattopadhyay
authors.forename: Evgeni V
authors.initials: EV
authors.lastname: Sokurenko
mesh_terms: D000220:Adaptation, Biological; D018830:Adhesins, Escherichia coli / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology; D036781:Fimbriae Proteins / Q000235:genetics; D024841:Fluoroquinolones / Q000494:pharmacology; D014644:Genetic Variation; D059765:Homologous Recombination; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D018830:Adhesins, Escherichia coli; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D024841:Fluoroquinolones; C058769:fimH protein, E coli; D036781:Fimbriae Proteins