title: Slow-binding inhibition of soybean lipoxygenase-1 by luteolin.
abstract: Luteolin, isolated from the seeds of Perilla frutescens (perilla seeds), inhibited the peroxidation of linoleic acid catalyzed by soybean lipoxygenase-1 (EC 1.13.11.12, Type 1) with an IC(50) of 5.0 M (1.43 μg/mL) noncompetitively. The progress curves for an enzyme reaction indicate that luteolin shows slow binding kinetics. Both the initial velocity and steady-state rate in the progress curve were decreased with increasing the concentration of luteolin. The kinetic parameters, which described the inhibition by luteolin, were evaluated by nonlinear regression fits.
authors.affiliation: Department of Functional Crop, National Institute of Crop Science, Miryang, Korea.
authors.forename: Tae Joung
authors.initials: TJ
authors.lastname: Ha
authors.forename: Myoung-Hee
authors.initials: MH
authors.lastname: Lee
authors.forename: Hyun-Tae
authors.initials: HT
authors.lastname: Kim
authors.forename: Hyun Sook
authors.initials: HS
authors.lastname: Kwon
authors.forename: In-Youl
authors.initials: IY
authors.lastname: Baek
authors.forename: Isao
authors.initials: I
authors.lastname: Kubo
authors.forename: Dae Sik
authors.initials: DS
authors.lastname: Jang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects; D008084:Lipoxygenase / Q000378:metabolism*; D016859:Lipoxygenase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D047311:Luteolin / Q000302:isolation & purification / Q000494:pharmacology*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D031343:Perilla / Q000737:chemistry*; D011485:Protein Binding; D012639:Seeds / Q000737:chemistry; D013025:Glycine max / Q000201:enzymology*; D013997:Time Factors
chemical_list: D016859:Lipoxygenase Inhibitors; D008084:Lipoxygenase; D047311:Luteolin