title: Development of enzyme-linked immunosorbent assay for the detection of Bt protein in transgenic cotton.
abstract: Enzyme-linked immunosorbent assays (ELISA) are being used extensively for the identification of Bt-protein in Bt transgenic crops. A sandwich ELISA test is the most preferable immunoassay for the quantification of Bt-protein in transgenic cotton plants. Here, we describe development of sandwich ELISA, employing polyclonal rabbit antibody as a capture antibody and HRP-labeled mouse anti-Bt protein-antibody as a detector antibody.
authors.affiliation: Molecular Genomics Laboratory, National Institute of Immunology, New Delhi, India. suchitrakamle@gmail.com
authors.forename: Suchitra
authors.initials: S
authors.lastname: Kamle
authors.forename: Abhishek
authors.initials: A
authors.lastname: Ojha
authors.forename: Arvind
authors.initials: A
authors.lastname: Kumar
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D001413:Bacillus thuringiensis / Q000235:genetics; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D002138:Calibration; D018556:Crops, Agricultural / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D003368:Gossypium / Q000235:genetics*; D030821:Plants, Genetically Modified
chemical_list: D000906:Antibodies; D001426:Bacterial Proteins