title: Rapid characterization of mitochondrial genome rearrangements in Australian songbirds using next-generation sequencing technology.
abstract: Using next-generation sequencing technology, we describe the complete mitochondrial genomes for 5 Australian passerine birds (Epthianura albifrons, Petroica phoenicea, Petroica goodenovii, Petroica boodang, and Eopsaltria australis). We successfully assemble each mitogenome de novo using just 1/8th of a Roche GL FSX 454 pyrosequencing plate. From the assembled mitogenomes, we identify 2 different mitochondrial gene arrangements in the region spanning 5'-3' from Cytochrome B to 12s RNA. These gene arrangements represent 2 of the 4 known avian mitochondrial gene arrangements. Our results, together with other previously described avian mitogenomes, highlight that certain mitochondrial rearrangements appear to have arisen multiple times.
authors.affiliation: Australian Museum, 6 College Street, Sydney, NSW 2010, Australia.
authors.forename: Georgina M
authors.initials: GM
authors.lastname: Cooke
authors.forename: Andrew G
authors.initials: AG
authors.lastname: King
authors.forename: Rebecca N
authors.initials: RN
authors.lastname: Johnson
authors.forename: Walter E
authors.initials: WE
authors.lastname: Boles
authors.forename: Richard E
authors.initials: RE
authors.lastname: Major
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics; D000818:Animals; D045303:Cytochromes b / Q000235:genetics; D023061:Gene Order; D054629:Genome, Mitochondrial; D008969:Molecular Sequence Data; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012343:RNA, Transfer; D017422:Sequence Analysis, DNA / Q000379:methods; D020308:Songbirds / Q000235:genetics*
chemical_list: D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C075526:RNA, ribosomal, 12S; D012343:RNA, Transfer; D045303:Cytochromes b; D000251:Adenosine Triphosphatases