title: Concentrations of MMP-9 and TIMP-1 in lip tissue and their impact on cleft lip surgery healing.
abstract: OBJECTIVE
  To compare aspects of wound healing after cleft lip surgery performed within one week of age and wound healing after surgery performed within 2 - 4 months of age, especially concentrations of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinases-1 (TIMP-1) in tissue removed during surgery.
  
  
  METHODS
  34 tissue samples (26 boys and 8 girls) were removed during surgery within one week of age (n=19) or within 2 - 4 months of age (n=15). Tissue samples were separated into epidermis, dermis and mucous membrane. Proteins were extracted in cacodylic buffer for 24 h at a temperature 2 - 8 ºC. Total protein concentrations were examined using a modification of the Lowry method. Samples were examined using ELISA kit Amersham Biotrak Activity Assay (GE Healthcare UK) for detection of MMP-9 and TIMP-1 concentrations.
  
  
  RESULTS
  MMP-9: early surgery - epidermis 2.168 ± 3.303 μg/g of protein (mean ± SD), dermis 1.251 ± 1.848 µg/g, 2 - 4 months surgery - epidermis 0.347 ± 0.212 μg/g, dermis 0.555 ± 0.276 µg/g. TIMP-1: early surgery - epidermis 1.762 ± 2.162 μg/g, dermis 1.628 ± 0.822 µg/g, mucous membrane 2.066 ± 1.717 µg/g, 2 - 4 months surgery - epidermis 1.881 ± 2.810 μg/g, dermis 3.117 ± 1.540 µg/g, mucous membrane 4.833 ± 6.550 µg/g.
  
  
  CONCLUSIONS
  There were no significant differences in concentrations of protein MMP-9 in epidermis and dermis and TIMP-1 in epidermis and mucous membrane according to time of surgery. Significantly decreased levels of TIMP-1 in dermis were found in samples obtained from early surgery compared to levels in samples obtained from 2 - 4 months surgery.
authors.affiliation: Department of Clinical Biochemistry and Pathobiochemistry, 2nd Faculty of Medicine, Charles University in Prague and Faculty Hospital Motol, Prague, Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Blaha
authors.forename: Jiri
authors.initials: J
authors.lastname: Borsky
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kasparova
authors.forename: Anna
authors.initials: A
authors.lastname: Steklacova
authors.forename: Veronika
authors.initials: V
authors.lastname: Zajickova
authors.forename: Marta
authors.initials: M
authors.lastname: Pechova
authors.forename: Radka
authors.initials: R
authors.lastname: Matejova
authors.forename: Karel
authors.initials: K
authors.lastname: Kotaska
authors.forename: Tatjana
authors.initials: T
authors.lastname: Dostalova
mesh_terms: D002971:Cleft Lip / Q000601:surgery*; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008046:Lip / Q000737:chemistry*; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000032:analysis*; D019715:Tissue Inhibitor of Metalloproteinase-1; D014945:Wound Healing
chemical_list: C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D020780:Matrix Metalloproteinase 9