title: Late onset vascular dysfunction in the R6/1 model of Huntington's disease.
abstract: Huntington's disease is a neurodegenerative disorder that also gives raise to widespread changes in peripheral organs and tissues. We tested the hypothesis that vascular dysfunction may occur in Huntington's disease by studying R6/1 mice which express exon 1 of the mutant huntingtin gene. We assessed arterial function in R6/1 and wild type (WT) mice using myography. Arterial contractility was largely unaltered in R6/1 arteries at 15 and 32 weeks of age. By 40 weeks, contractility was impaired irrespective of which vasoconstrictor we tested. Endothelium-dependent relaxation was not affected, and we observed no changes in arterial geometry or expression of contractile proteins, such as myosin regulatory light chains or smooth muscle α-actin. The frequency of calcium oscillations in R6/1 arterial smooth muscle cells was higher than in WT control tissue, whereas myosin phosphorylation was unaltered. Impairment of force by the mitochondrial inhibitors cyanide and rotenone was less pronounced in R6/1 than in WT arteries and mitochondria were enlarged, in keeping with an effect related to altered mitochondrial function. Our results reveal that arteries in the R6/1 model of Huntington's disease exhibit an age-dependent impairment of contractility and that they depend less on mitochondrial function when they contract.
authors.affiliation: Section for Cell and Tissue Biology, Department of Experimental Medical Science, Lund University, BMC D12, Sweden. Awahan.Reheman@ki.se
authors.forename: Awahan
authors.initials: A
authors.lastname: Rahman
authors.forename: Mari
authors.initials: M
authors.lastname: Ekman
authors.forename: Yulia
authors.initials: Y
authors.lastname: Shakirova
authors.forename: Kristina E
authors.initials: KE
authors.lastname: Andersson
authors.forename: Matthias
authors.initials: M
authors.lastname: Mörgelin
authors.forename: Jonas S
authors.initials: JS
authors.lastname: Erjefält
authors.forename: Patrik
authors.initials: P
authors.lastname: Brundin
authors.forename: Jia-Yi
authors.initials: JY
authors.lastname: Li
authors.forename: Karl
authors.initials: K
authors.lastname: Swärd
mesh_terms: D000818:Animals; D001158:Arteries / Q000503:physiopathology*; D020013:Calcium Signaling / Q000187:drug effects; D003486:Cyanides / Q000494:pharmacology; D005260:Female; D006801:Humans; D006816:Huntington Disease / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D032342:Muscle Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D009213:Myography; D012402:Rotenone / Q000494:pharmacology; D013997:Time Factors; D014661:Vasoconstriction / Q000187:drug effects
chemical_list: D003486:Cyanides; D012402:Rotenone