title: UFLC-MS/MS method for simultaneous determination of luteolin-7-O-gentiobioside, luteolin-7-O-β-D-glucoside and luteolin-7-O-β-D-glucuronide in beagle dog plasma and its application to a pharmacokinetic study after administration of traditional Chinese medicinal preparation: Kudiezi injection.
abstract: A rapid, sensitive and selective ultra-fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) method for the simultaneous determination of three active flavonoid glycosides: luteolin-7-O-gentiobioside, luteolin-7-O-β-D-glucoside and luteolin-7-O-β-D-glucuronide in beagle dog plasma was developed and validated. Puerarin was used as internal standard (IS). After protein precipitation with acidified acetonitrile, the analytes were separated on a Venusil MP C18 column with a gradient elution system composed of 0.05% formic acid and acetonitrile at a flow rate of 0.4ml/min. Detection was performed using multiple reaction monitoring (MRM) mode with a turbo ion spray source under a negative ionization condition. The calibration curves of the three analytes showed good linearity (r>0.995) within the tested concentration ranges. The lower limits of quantification for luteolin-7-O-gentiobioside, luteolin-7-O-β-D-glucoside and luteolin-7-O-β-D-glucuronide were 1.0 ng/ml, 1.0 ng/ml and 4.0 ng/ml, respectively. The intra-day and inter-day precision and accuracy deviations were less than 15%, and the extraction recoveries of the three analytes from beagle dog plasma were more than 75%. The validated method was successfully applied to a pharmacokinetic study of the three flavonoid glycosides in beagle dog plasma after intravenous administration of the traditional Chinese medicinal preparation: Kudiezi injection.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, PR China.
authors.forename: Ran
authors.initials: R
authors.lastname: Yin
authors.forename: Fei
authors.initials: F
authors.lastname: Han
authors.forename: Zheng
authors.initials: Z
authors.lastname: Tang
authors.forename: Ran
authors.initials: R
authors.lastname: Liu
authors.forename: Xu
authors.initials: X
authors.lastname: Zhao
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Chen
authors.forename: Kaishun
authors.initials: K
authors.lastname: Bi
mesh_terms: D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D047309:Flavones / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D005419:Flavonoids / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D005960:Glucosides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D007529:Isoflavones / Q000737:chemistry; D047311:Luteolin / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D008516:Medicine, Chinese Traditional / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D047309:Flavones; D005419:Flavonoids; D005960:Glucosides; D007529:Isoflavones; C456096:luteolin-7-O-glucuronide; C066408:luteolin-7-glucoside; D047311:Luteolin; C033607:puerarin