title: Association between genetic polymorphisms of NOD 1 and Helicobacter pylori-induced gastric mucosal inflammation in healthy Korean population.
abstract: BACKGROUND
  Gastric cancer is supposed to be a result of inflammation induced by Helicobacter pylori (H. pylori) infection. Nucleotide-binding oligomerization domain 1 (NOD 1) is required for the innate immune response to H. pylori. We aim to investigate whether single nucleotide polymorphism (SNP) in NOD 1 gene is associated with H. pylori-induced gastric mucosal inflammation in a healthy Korean population.
  
  
  METHODS
  The study was conducted on 412 adults who visited two different healthcare centers for health examinations. The G796A (E266K) NOD 1 SNP was detected by using polymerase chain reaction/restriction fragment length polymorphism. A gastritis score was calculated by the summed values of the grade and the activity of gastritis scored according to the updated Sydney system. The expression of IL-8 and COX-2 mRNA was assessed by quantitative reverse transcription polymerase chain reaction. In the group with H. pylori infection, the complete screening of the genes comprising the cag PAI was performed.
  
  
  RESULTS
  The genotype frequencies were 26.7% (AA type), 58.3% (GA), and 15.0% (GG). In H. pylori-positive patients, gastritis score of the AA genotype was significantly higher than those of the others (p = .04). Also, the IL-8 and COX-2 mRNA levels increased in the AA genotype. In the group with H. pylori infection, 31.9% were found to carry the complete cag PAI. When the subjects were infected with intact cag PAI, the IL-8 and COX-2 mRNA levels were significantly high in AA genotype.
  
  
  CONCLUSIONS
  G796A (E266K) NOD 1 polymorphism is closely correlated with H. pylori-associated gastric mucosal inflammation in the Korean population.
authors.affiliation: Internal Medicine, The Catholic University of Korea, Suwon, Korea.
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Kim
authors.forename: Jeong Rok
authors.initials: JR
authors.lastname: Lee
authors.forename: Woo Chul
authors.initials: WC
authors.lastname: Chung
authors.forename: Sung Hoon
authors.initials: SH
authors.lastname: Jung
authors.forename: Hae Jung
authors.initials: HJ
authors.lastname: Sung
authors.forename: Yang Woon
authors.initials: YW
authors.lastname: Lee
authors.forename: You Suk
authors.initials: YS
authors.lastname: Oh
authors.forename: Sang Bae
authors.initials: SB
authors.lastname: Kim
authors.forename: Chang Nyol
authors.initials: CN
authors.lastname: Paik
authors.forename: Kang-Moon
authors.initials: KM
authors.lastname: Lee
authors.forename: Seung June
authors.initials: SJ
authors.lastname: Noh
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D005260:Female; D005756:Gastritis / Q000235:genetics* / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D020022:Genetic Predisposition to Disease; D016481:Helicobacter Infections / Q000235:genetics* / Q000276:immunology / Q000382:microbiology / Q000503:physiopathology; D016480:Helicobacter pylori / Q000472:pathogenicity; D006801:Humans; D007723:Korea; D008297:Male; D008875:Middle Aged; D053474:Nod1 Signaling Adaptor Protein / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D055815:Young Adult
chemical_list: C119067:NOD1 protein, human; D053474:Nod1 Signaling Adaptor Protein