title: HuR posttranscriptionally regulates early growth response-1 (Egr-1) expression at the early stage of T cell activation.
abstract: T cell activation depends on appropriate and precise regulation of gene expression. Here we find that rapidly translocated RNA-binding protein HuR, forms messenger ribonucleoprotein (mRNP) complexes with transiently expressed mRNAs encoding early-response transcription factors, including c-Fos, c-Jun, and Egr-1. Knockdown and overexpression assays demonstrated that proper posttranscriptional control of Egr-1 expression requires HuR-mediated translation control. Further analysis showed that the Egr-1 3'UTR, which contains AU-rich elements (AREs) and interacts directly with HuR, suppresses reporter gene expression and mediates posttranscriptional regulation of Egr-1 by HuR. These findings underscore an essential role for HuR in regulating early events during T cell activation.
authors.affiliation: Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences (CAS) & Shanghai Jiao Tong University School of Medicine (SJTUSM), 225 South Chongqing Road, Shanghai 200025, China.
authors.forename: Zongchun
authors.initials: Z
authors.lastname: Mou
authors.forename: Jia
authors.initials: J
authors.lastname: You
authors.forename: Qianghai
authors.initials: Q
authors.lastname: Xiao
authors.forename: Youheng
authors.initials: Y
authors.lastname: Wei
authors.forename: Juan
authors.initials: J
authors.lastname: Yuan
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Gary
authors.initials: G
authors.lastname: Brewer
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Ma
mesh_terms: D020413:3' Untranslated Regions / Q000502:physiology; D051959:ELAV Proteins / Q000378:metabolism*; D051766:Early Growth Response Protein 1 / Q000096:biosynthesis*; D051765:Early Growth Response Transcription Factors / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D019169:Jurkat Cells; D008213:Lymphocyte Activation; D012323:RNA Processing, Post-Transcriptional; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D020413:3' Untranslated Regions; C497325:EGR1 protein, human; D051959:ELAV Proteins; D051766:Early Growth Response Protein 1; D051765:Early Growth Response Transcription Factors; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C026452:messenger ribonucleoprotein