title: Neutral lipid metabolism influences phospholipid synthesis and deacylation in Saccharomyces cerevisiae.
abstract: Establishment and maintenance of equilibrium in the fatty acid (FA) composition of phospholipids (PL) requires both regulation of the substrate available for PL synthesis (the acyl-CoA pool) and extensive PL turnover and acyl editing. In the present study, we utilize acyl-CoA synthetase (ACS) deficient cells, unable to recycle FA derived from lipid deacylation, to evaluate the role of several enzymatic activities in FA trafficking and PL homeostasis in Saccharomyces cerevisiae. The data presented show that phospholipases B are not contributing to constitutive PL deacylation and are therefore unlikely to be involved in PL remodeling. In contrast, the enzymes of neutral lipid (NL) synthesis and mobilization are central mediators of FA trafficking. The phospholipid:DAG acyltransferase (PDAT) Lro1p has a substantial effect on FA release and on PL equilibrium, emerging as an important mediator in PL remodeling. The acyl-CoA dependent biosynthetic activities of NL metabolism are also involved in PL homeostasis through active modulation of the substrate available for PL synthesis. In addition TAG mobilization makes an important contribution, especially in cells from stationary phase, to FA availability. Beyond its well-established role in the formation of a storage pool, NL metabolism could play a crucial role as a mechanism to uncouple the pools of PL and acyl-CoAs from each other and thereby to allow independent regulation of each one.
authors.affiliation: Department of Plant Biochemistry, Albrecht-von-Haller Institute, Georg-August University Goettingen, Goettingen, Germany.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Mora
authors.forename: Michael
authors.initials: M
authors.lastname: Scharnewski
authors.forename: Martin
authors.initials: M
authors.lastname: Fulda
mesh_terms: D000215:Acylation; D004952:Esters / Q000378:metabolism; D050612:Fatty Acid Transport Proteins / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D006706:Homeostasis; D050356:Lipid Metabolism; D008245:Lysophospholipase / Q000172:deficiency / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D010743:Phospholipids / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D004952:Esters; C105342:FAT1 protein, S cerevisiae; D050612:Fatty Acid Transport Proteins; D005227:Fatty Acids; D010743:Phospholipids; D029701:Saccharomyces cerevisiae Proteins; D014280:Triglycerides; D008245:Lysophospholipase