title: The conserved oligomeric Golgi complex is involved in penetration resistance of barley to the barley powdery mildew fungus.
abstract: Membrane trafficking is vital to plant development and adaptation to the environment. It is suggested that post-Golgi vesicles and multivesicular bodies are essential for plant defence against directly penetrating fungal parasites at the cell wall. However, the actual plant proteins involved in membrane transport for defence are largely unidentified. We applied a candidate gene approach and single cell transient-induced gene silencing for the identification of membrane trafficking proteins of barley involved in the response to the fungal pathogen Blumeria graminis f.sp. hordei. This revealed potential components of vesicle tethering complexes [putative exocyst subunit HvEXO70F-like and subunits of the conserved oligomeric Golgi (COG) complex] and Golgi membrane trafficking (COPIγ coatomer and HvYPT1-like RAB GTPase) as essential for resistance to fungal penetration into the host cell.
authors.affiliation: Lehrstuhl für Phytopathologie, Technische Universität München, Freising-Weihenstephan, Germany.
authors.forename: Maya
authors.initials: M
authors.lastname: Ostertag
authors.forename: Johanna
authors.initials: J
authors.lastname: Stammler
authors.forename: Dimitar
authors.initials: D
authors.lastname: Douchkov
authors.forename: Ruth
authors.initials: R
authors.lastname: Eichmann
authors.forename: Ralph
authors.initials: R
authors.lastname: Hückelhoven
mesh_terms: D001203:Ascomycota / Q000472:pathogenicity*; D006056:Golgi Apparatus / Q000378:metabolism*; D001467:Hordeum / Q000378:metabolism* / Q000382:microbiology*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D057865:Plant Immunity / Q000235:genetics / Q000502:physiology*