title: A novel molecular beacon bearing a graphite nanoparticle as a nanoquencher for in situ mRNA detection in cancer cells.
abstract: Molecular beacons (MBs) have shown fascinating applications in many biological fields. However, exploration of cost-effective, sensitive, stable and efficient MB for in situ live cell- based assay has still room for improvement. In this regards, we have developed a novel MB which bears a spherical graphite nanoparticle (GN) as a fluorescent quencher. The GN resulted in the high quenching efficiency, and the presence of GN enhanced the biological stability and transfection of the MB into the cells, thereby enabling the real-time survivin mRNA detection and quantification in the MCF-7 breast cancer cells. These results demonstrated that the advancement of the proposed MB containing a GN nanoquencher can be used as a robust molecular probe for genetic analysis in the cells.
authors.affiliation: Department of Chemical and Biomolecular Engineering (BK21 program), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Yunxian
authors.initials: Y
authors.lastname: Piao
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.forename: Tae Seok
authors.initials: TS
authors.lastname: Seo
mesh_terms: D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D017931:DNA Primers; D005260:Female; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D012333:RNA, Messenger / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D006108:Graphite