title: Comprehensive analysis of Phyteuma orbiculare L., a wild Alpine food plant.
abstract: Plants which have been traditionally eaten by the alpine population may provide new opportunities for agricultural development in mountain regions. In this context we have investigated the chemical composition of Phyteuma orbiculare (Campanulaceae), a perennial herb whose leaves have been eaten as salad by rural populations in Valais (Switzerland). Extracts of different polarities were subjected to comprehensive metabolite profiling using a dereplication platform combining HPLC-PDA-MS, and offline NMR analysis. Twenty-three compounds, including various phenolic glycosides, a new dimeric phenylpropanoid glucoside, saponins, and fatty acids were identified online, or after targeted isolation. Selected phenolic constituents were quantitatively assessed by HPLC-PDA analysis. In addition, substances relevant for nutrition, such as β-carotene, fatty acids, ascorbic acid and minerals were quantified in leaves and flowers. The antioxidant capacity was determined with an ORAC assay, and total phenolic compounds were quantified. Finally, the phytochemical profile was compared to that of the related species P. spicatum, P. hemisphaericum and P. ovatum.
authors.affiliation: Division of Pharmaceutical Biology, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50, CH-4056 Basel, Switzerland.
authors.forename: Christian
authors.initials: C
authors.lastname: Abbet
authors.forename: Ivan
authors.initials: I
authors.lastname: Slacanin
authors.forename: Matthias
authors.initials: M
authors.lastname: Hamburger
authors.forename: Olivier
authors.initials: O
authors.lastname: Potterat
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000378:metabolism; D029422:Campanulaceae / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism; D035264:Flowers / Q000737:chemistry; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000032:analysis* / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry; D013557:Switzerland; D019207:beta Carotene / Q000032:analysis / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005227:Fatty Acids; D010936:Plant Extracts; D019207:beta Carotene