title: A potential probiotic culture ST2 produces siderophore 2,3-dihydroxybenzoylserine under intestinal conditions.
abstract: The aim of this study was to characterise siderophores of a culture bearing better probiotic qualities of human origin. In this direction, bacterial strain ST2 was isolated from faecal sample of 6-month-old healthy infant, which showed close similarity with Escherichia coli based on 16S r-RNA gene sequence analysis and biochemical characteristics. It was tested for siderophore production under in vitro iron limiting intestinal conditions. The siderophore produced was catecholate type of 2,3-dihydroxybenzoylserine on the basis of FTIR, NMR and mass spectra analysis. The strain obeyed probiotic properties as per WHO and FAO guidelines. It showed excellent tolerance in partially simulated gastro-intestinal tract conditions, non-haemolytic nature, susceptibility to many antibiotics tested and antimicrobial activity against enteric pathogens like Salmonella typhimurium, Streptococcus pyogenes, and Staphylococcus aureus. Hence, this culture could be a possible probiotic candidate in iron nutrition, but only after conducting proper animal and human trials.
authors.affiliation: Department of Microbiology, School of Life Sciences, North Maharashtra University, P.O. Box-80, Jalgaon 425 001, India.
authors.forename: Jayesh J
authors.initials: JJ
authors.lastname: Ahire
authors.forename: Kanchankumar P
authors.initials: KP
authors.lastname: Patil
authors.forename: Bhushan L
authors.initials: BL
authors.lastname: Chaudhari
authors.forename: Sudhir B
authors.initials: SB
authors.lastname: Chincholkar