title: Effect of supplementation of the laying hen diet with olive leaves (Olea europea L.) on lipid oxidation and fatty acid profile of α-linolenic acid enriched eggs during storage.
abstract: 1. The aim of this study was to evaluate the effect of supplementation of the layer diet with olive leaves (Olea europea L.) on lipid oxidation and fatty acid profile of α-linolenic acid enriched eggs during refrigerated storage, and to compare this effect with α-tocopheryl acetate supplementation.   2. A total of 72 brown Lohmann laying hens, equally allocated to 3 groups, were fed on diets supplemented with 40 g/kg linseed oil, or linseed oil and olive leaves at 10 g/kg or linseed oil and α-tocopheryl acetate at 200 mg/kg. Collected eggs were analysed for fatty acid profile and lipid oxidation either fresh or following 60 d storage at 4°C.   3. Results showed that olive leaves or α-tocopheryl acetate supplementation reduced lipid hydroperoxide concentration in fresh eggs but had no effect on their fatty acid profile and malondialdehyde (MDA) content compared to controls.   4. Refrigerated storage for 60 d decreased the proportions of PUFAs but increased those of MUFAs in eggs from the control diet, whilst it had no effect on the fatty acid composition of eggs from the diets supplemented with olive leaves or α-tocopheryl acetate, which in turn showed decreased concentrations of lipid hydroperoxides and MDA.
authors.affiliation: Laboratory of Hygiene of Foods of Animal Origin, Faculty of Veterinary Medicine, University of Thessaly, 43100 Karditsa.
authors.forename: E
authors.initials: E
authors.lastname: Botsoglou
authors.forename: A
authors.initials: A
authors.lastname: Govaris
authors.forename: D
authors.initials: D
authors.lastname: Fletouris
authors.forename: N
authors.initials: N
authors.lastname: Botsoglou
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002645:Chickens / Q000378:metabolism*; D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D003080:Cold Temperature; D019587:Dietary Supplements / Q000032:analysis; D004531:Eggs / Q000032:analysis*; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D008043:Linseed Oil / Q000737:chemistry / Q000378:metabolism; D050356:Lipid Metabolism; D031658:Olea / Q000737:chemistry*; D010063:Ovum / Q000378:metabolism; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000008:administration & dosage; D018515:Plant Leaves / Q000737:chemistry; D013053:Spectrophotometry; D017962:alpha-Linolenic Acid / Q000008:administration & dosage / Q000378:metabolism*; D024502:alpha-Tocopherol / Q000008:administration & dosage / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D010936:Plant Extracts; D017962:alpha-Linolenic Acid; D008043:Linseed Oil; D024502:alpha-Tocopherol