title: The nuclear factor-κB correlates with increased expression of interleukin-6             and promotes progression of gastric carcinoma.
abstract: The interleukin-6 (IL-6) pathway is one of the mechanisms that link inflammation             and angiogenesis with malignancy. Since nuclear factor-κB (NF-κB) is a potential             sign for inflammation, NF-κB has been associated with the progression of disease             in various types of cancer. In the present study, we investigated the effect of             NF-κB on the IL-6 pathway in gastric carcinoma and their correlation with disease             status and prognosis. The mRNA and protein levels of NF-κB, IL-6 and vascular             endothelial growth factor (VEGF) were detected by western blotting and reverse             transcription (RT) quantitative PCR (RT-qPCR). Using immunohistochemistry, we             examined the expression of these proteins in normal and human gastric cancer tissue             samples. The concentrations of IL-6 and TNF-α in collected blood samples were             measured according to the enzyme-linked immunosorbent assay (ELISA). IL-6 and             TNF-α were found to be expressed at high levels in human gastric cancer samples.             A positive correlation was found between the expression of IL-6 and NF-κB by immunohistochemical             and further correlation analysis. IL-6, NF-κB and VEGF protein and mRNA levels             increased significantly in gastric cancer tissue compared with those in adjacent             normal mucosa tissue samples. In conclusion, our findings demonstrate that NF-κB,             IL-6 and VEGF mRNA and protein levels increase significantly in gastric cancer             tissues. In addition, the expression of NF-κB was positively correlated with the             expression of IL-6 according to immunohistochemical and further correlation analysis,             which suggests that the suppression of NF-κB or IL-6 may be a potential target             for clinical therapy of gastric cancer in the future.
authors.affiliation: Department of Plastic and Reconstruction Surgery, Beijing Luhe Hospital, Capital Medical University, Beijing 101199, PR China.
authors.forename: Yefeng
authors.initials: Y
authors.lastname: Yin
authors.forename: Xiulian
authors.initials: X
authors.lastname: Si
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.forename: Lei
authors.initials: L
authors.lastname: Gao
authors.forename: Jiangning
authors.initials: J
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D015850:Interleukin-6 / Q000097:blood* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D015850:Interleukin-6; D016328:NF-kappa B; D012333:RNA, Messenger; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A