title: Hyperthermia inhibits the proliferation and invasive ability of mouse malignant melanoma through TGF-β(1).
abstract: The degradation of basement membranes by tumor cells involves secretion and activation of proteinases, such as the matrix metalloproteinases (MMPs), and results from an imbalance between their inhibitors and activators that are controlled by various growth factors or cytokines, among which TGF-β(1) may be the most intriguing. In order to study the therapeutic effect and molecular mechanism of hyperthermia on aggressive malignant melanoma, the expression levels of TGF-β(1) and Smad4 in B16F10 cells were dynamically analyzed by RT-PCR and western blotting for 24 h after heat treatment, from which time-dependent changes were determined. As expected, the proliferation and invasive ability of B16F10 cells were suppressed strongly by heat treatment. Furthermore, we compared the expression of TGF-β(1) in melanoma mouse models before and after magnetic fluid hyperthermia (MFH) in vivo. After hyperthermia, the tumor growth rate was reduced with a decline in TGF-β(1) protein expression. We conclude that changes in the TGF-β(1) pathway induced by hyperthermia may be an important part of the molecular mechanism involved.
authors.affiliation: Xiangya Hospital of Central South University, Changsha 410008, PR China.
authors.forename: Hekun
authors.initials: H
authors.lastname: Jin
authors.forename: Xiaoxue
authors.initials: X
authors.lastname: Xie
authors.forename: Bingqiang
authors.initials: B
authors.lastname: Hu
authors.forename: Fuping
authors.initials: F
authors.lastname: Gao
authors.forename: Jumei
authors.initials: J
authors.lastname: Zhou
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lehui
authors.initials: L
authors.lastname: Du
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Wang
authors.forename: Lingyun
authors.initials: L
authors.lastname: Zhao
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhang
authors.forename: Liangfang
authors.initials: L
authors.lastname: Shen
authors.forename: Yuping
authors.initials: Y
authors.lastname: Liao
authors.forename: Jintian
authors.initials: J
authors.lastname: Tang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D003094:Collagen; D004338:Drug Combinations; D005260:Female; D006979:Hyperthermia, Induced; D007797:Laminin; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008546:Melanoma, Experimental / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D009361:Neoplasm Invasiveness; D011509:Proteoglycans; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D051901:Smad4 Protein / Q000378:metabolism; D016019:Survival Analysis; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D012333:RNA, Messenger; D051901:Smad4 Protein; C490810:Smad4 protein, mouse; D053773:Transforming Growth Factor beta1; C059485:matrigel; D003094:Collagen; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9