title: An eceriferum locus, cer-zv, is associated with a defect in cutin responsible for water retention in barley (Hordeum vulgare) leaves.
abstract: Drought limits plant growth and threatens crop productivity. A barley (Hordeum vulgare) ethylene imine-induced monogenic recessive mutant cer-zv, which is sensitive to drought, was characterized and genetically mapped in the present study. Detached leaves of cer-zv lost 34.2 % of their initial weight after 1 h of dehydration. The transpiration was much higher in cer-zv leaves than in wild-type leaves under both light and dark conditions. The stomata of cer-zv leaves functioned normally, but the cuticle of cer-zv leaves showed increased permeability to ethanol and toluidine blue dye. There was a 50-90 % reduction in four major cutin monomers, but no reduction in wax loads was found in the cer-zv mutant as compared with the wild type. Two F(2) mapping populations were established by the crosses of 23-19 × cer-zv and cer-zv × OUH602. More polymorphisms were found in EST sequences between cer-zv and OUH602 than between cer-zv and 23-19. cer-zv was located in a pericentromeric region on chromosome 4H in a 10.8 cM interval in the 23-19 × cer-zv map based on 186 gametes tested and a 1.7 cM interval in the cer-zv × OUH602 map based on 176 gametes tested. It co-segregated with EST marker AK251484 in both maps. The results indicated that the cer-zv mutant is defective in cutin, which might be responsible for the increased transpiration rate and drought sensitivity, and that the F(2) of cer-zv × OUH602 might better facilitate high resolution mapping of cer-zv.
authors.affiliation: Laboratory of Plant Stress Ecophysiology and Biotechnology, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Donggang West Road 320, Lanzhou, 730000, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.forename: Aidong
authors.initials: A
authors.lastname: Wang
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Ma
authors.forename: Mohammad
authors.initials: M
authors.lastname: Pourkheirandish
authors.forename: Shun
authors.initials: S
authors.lastname: Sakuma
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Shunzong
authors.initials: S
authors.lastname: Ning
authors.forename: Eviatar
authors.initials: E
authors.lastname: Nevo
authors.forename: Christiane
authors.initials: C
authors.lastname: Nawrath
authors.forename: Takao
authors.initials: T
authors.lastname: Komatsuda
authors.forename: Guoxiong
authors.initials: G
authors.lastname: Chen
mesh_terms: D002734:Chlorophyll / Q000378:metabolism; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D018744:DNA, Plant / Q000235:genetics; D020224:Expressed Sequence Tags; D017343:Genes, Plant; D008040:Genetic Linkage; D056426:Genetic Loci; D005819:Genetic Markers; D005838:Genotype; D001467:Hordeum / Q000235:genetics* / Q000254:growth & development; D008563:Membrane Lipids / Q000378:metabolism*; D009154:Mutation; D018515:Plant Leaves / Q000235:genetics* / Q000254:growth & development; D014867:Water / Q000378:metabolism; D014885:Waxes / Q000378:metabolism
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers; D008563:Membrane Lipids; D014885:Waxes; D014867:Water; D002734:Chlorophyll; C000521:cutin