title: Presence of adrenergic receptors in rat endolymphatic sac epithelial cells.
abstract: Intravenous application of catecholamines produces a depression in the endolymphatic sac direct current potential (ESP) and increases endolymphatic pressure via the β-adrenergic receptor (AR) in guinea pigs, suggesting that catecholamines play a role in the endolymphatic system. However, the localization of ARs in the endolymphatic sac (ES) is still undetermined. The presence of ARs in the rat ES was investigated by reverse transcriptase-polymerase chain reaction using laser capture microdissection (LCM) and immunohistochemical analysis. Expression of α(1A)-, α(1B)-, α(2A)-, α(2B)-, β(1)-, β(2)- and β(3)-ARs was observed in LCM samples of ES epithelia. Immunohistochemical analysis using specific antibodies showed immunofluorescence of β(2)- and β(3)-ARs in epithelial cells of the ES intermediate portion, and no specific staining results were obtained for α(1)-, α(2A)-, α(2B)- and β(1)-ARs. The presence of β(2)-AR with no clear immunostaining of β(1)-AR in ES epithelial cells is in accordance with previous electrophysiological and pharmacological results, which suggests that β(2)-AR mediates the action of catecholamines on the ESP. The presence of β(3)-AR in the ES epithelial cells and its absence in the stria vascularis implies that β(3)-AR plays a specific role in the ES.
authors.affiliation: Department of Otolaryngology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Kita-gun, Miki-cho, Kagawa 761-0793, Japan. aimatsu@med.kagawa-u.ac.jp
authors.forename: Ai
authors.initials: A
authors.lastname: Matsubara
authors.forename: Takenori
authors.initials: T
authors.lastname: Miyashita
authors.forename: Ryuhei
authors.initials: R
authors.lastname: Inamoto
authors.forename: Nozomu
authors.initials: N
authors.lastname: Mori
mesh_terms: D000818:Animals; D004712:Endolymphatic Sac / Q000166:cytology*; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D061346:Laser Capture Microdissection; D051381:Rats; D017207:Rats, Sprague-Dawley; D011941:Receptors, Adrenergic / Q000235:genetics / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D011941:Receptors, Adrenergic; D011943:Receptors, Adrenergic, beta