title: Iron-sulfur cluster scaffold (ISCU) gene is duplicated in salmonid fish and tissue and temperature dependent expressed in rainbow trout.
abstract: The iron-sulfur cluster protein ISCU is a scaffold protein tasked with the building and mediation of iron-sulfur [Fe-S]-clusters. These are crucial for [Fe-S]-enzymes, which are involved in essential biological cell processes like metabolism or ion transport. Analysis of ISCU in rainbow trout (Oncorhynchus mykiss) and maraena whitefish (Coregonus maraena) revealed the existence of two gene variants in each of the two salmonids. This study presents the characterization of the duplicated ISCU cDNA sequences in both species as well as the comparative functional analysis of the genes in healthy and affected fish of two rainbow trout strains differing in trait robustness under regional aquaculture conditions. Coding sequences of trout ISCUA and ISCUB genes are spanning over five exons. Open reading frames (ORF) of trout (ISCUA: 495bp, ISCUB: 498bp) and whitefish (ISCUA and ISCUB: 495bp) genes encode for evolutionary highly conserved proteins and share 72% sequence similarity with human ISCU. Transcriptome analyses comparing healthy fish of the local rainbow trout strain BORN and the import strain TCO revealed strain-specific expression patterns for ISCU. Expression analyses by quantitative RT-PCR indicated remarkable differences between the transcript level of the gene variants ISCUA and ISCUB. Moderate temperature challenge (8°C and 23°C) suggests a generally higher transcript level of the two gene variants at 8°C in the liver, spleen, and gill of both strains. However, no remarkable differences between the strains occurred in the temperature-dependent ISCU gene expression profiles. The experimental infection with Aeromonas salmonicida resulted in a different ISCU gene expression in the gill and trunk kidney of both strains after two weeks, suggesting a specific role of the scaffold gene in rainbow trout strain BORN, regarding the recovery after infection. Although results partially reflect the expected strain- and tissue-specific ISCUA and ISCUB regulation in rainbow trout, the data do not support the assumed association of ISCU with the trait robustness.
authors.affiliation: Leibniz-Institut für Nutztierbiologie (FBN), Fachbereich Molekularbiologie, Dummerstorf, Germany.
authors.forename: Marieke
authors.initials: M
authors.lastname: Verleih
authors.forename: Alexander
authors.initials: A
authors.lastname: Rebl
authors.forename: Bernd
authors.initials: B
authors.lastname: Köllner
authors.forename: Tomáš
authors.initials: T
authors.lastname: Korytář
authors.forename: Judith M
authors.initials: JM
authors.lastname: Köbis
authors.forename: Carsten
authors.initials: C
authors.lastname: Kühn
authors.forename: Klaus
authors.initials: K
authors.lastname: Wimmers
authors.forename: Tom
authors.initials: T
authors.lastname: Goldammer
mesh_terms: D048409:Aeromonas salmonicida / Q000378:metabolism; D000818:Animals; D029941:Fish Proteins / Q000096:biosynthesis* / Q000235:genetics; D005667:Furunculosis / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D020440:Gene Duplication; D005786:Gene Expression Regulation; D006358:Hot Temperature; D006801:Humans; D007506:Iron-Sulfur Proteins / Q000096:biosynthesis* / Q000235:genetics; D017686:Oncorhynchus mykiss / Q000235:genetics / Q000378:metabolism*; D016366:Open Reading Frames / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D059467:Transcriptome / Q000235:genetics
chemical_list: D029941:Fish Proteins; D007506:Iron-Sulfur Proteins