title: Lymphangiography of the mouse tail.
abstract: The lymphatic system is an important route for metastasis of cancer cells. Lymphatic microvessels have been studied by adapting lymphangiography to the mouse tail. In this technique, a high-molecular-weight fluorescently labeled tracer is injected into the interstitial compartment of the tail tissue. The tracer migrates to the local lymphatic vessels and fills the network, allowing visualization. By implanting a tumor in the tail, the structure and function of the lymphatics at the tumor periphery can be monitored. This protocol describes a method for injecting tumor cells into a mouse tail and for microlymphangiography.
authors.forename: Rakesh K
authors.initials: RK
authors.lastname: Jain
authors.forename: Lance L
authors.initials: LL
authors.lastname: Munn
authors.forename: Dai
authors.initials: D
authors.lastname: Fukumura
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes / Q000008:administration & dosage; D008220:Lymphography / Q000379:methods*; D051379:Mice; D013194:Staining and Labeling / Q000379:methods; D013623:Tail / Q000033:anatomy & histology*
chemical_list: D005456:Fluorescent Dyes