title: Characterization of isoforms of the lectin isolated from the red algae Bryothamnion seaforthii and its pro-healing effect.
abstract: Lectins are a structurally heterogeneous group of proteins that have specific binding sites for carbohydrates and glycoconjugates. Because of their biotechnological potential, lectins are widely used in biomedical research. The present study aimed to evaluate the healing potential of the lectin isolated from the marine red alga Bryothamnion seaforthii (BSL). The lectin was purified using ion exchange chromatography with DEAE cellulose and characterized using tandem mass spectrometry. For healing tests, skin wounds were induced in the dorsal thoracic region of mice. These animals were randomly divided into three groups and subjected to topical treatment for 12 days with BSL, bovine serum albumin and 150 mM NaCl. To evaluate the potential of each treatment, the animals were anesthetized and sacrificed on days 2, 7 and 12, respectively. The parameters evaluated included the wound area, the proportion of wound closure and the histological diagnosis. The wound closure was more effective with BSL (Postoperative Day 7 and 12) than controls. The luminal epithelium was completely restructured; the presence of collagen in the dermis and the strongly active presence of young skin annexes demonstrate the potential of treatment with BSL compared with controls. Our findings suggest that BSL has pro-healing properties and can be a potential medical process in the treatment of acute wounds.
authors.affiliation: LIBS, Integrated Laboratory of Biomolecules, Faculty of Medicine of Sobral, Federal University of Ceará, Fortaleza, CE 60020-181, Brazil.
authors.forename: Luiz
authors.initials: L
authors.lastname: Gonzaga do Nascimento-Neto
authors.affiliation: BioMol, Laboratory of Biologically Active Molecules, Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE 60020-181, Brazil.
authors.forename: Romulo Farias
authors.initials: RF
authors.lastname: Carneiro
authors.affiliation: BioMar, Laboratory of Marine Biotechnology, Department of Fishing Engineering, Federal University of Ceará, Fortaleza, CE 60020-181, Brazil.
authors.forename: Suzete Roberta
authors.initials: SR
authors.lastname: Da Silva
authors.affiliation: LIBS, Integrated Laboratory of Biomolecules, Faculty of Medicine of Sobral, Federal University of Ceará, Fortaleza, CE 60020-181, Brazil.
authors.forename: Bruno Rocha
authors.initials: BR
authors.lastname: Da Silva
authors.affiliation: LIBS, Integrated Laboratory of Biomolecules, Faculty of Medicine of Sobral, Federal University of Ceará, Fortaleza, CE 60020-181, Brazil.
authors.forename: Francisco Vassiliepe Sousa
authors.initials: FVS
authors.lastname: Arruda
authors.affiliation: BioMol, Laboratory of Biologically Active Molecules, Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE 60020-181, Brazil.
authors.forename: Victor Alves
authors.initials: VA
authors.lastname: Carneiro
authors.affiliation: BioMol, Laboratory of Biologically Active Molecules, Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE 60020-181, Brazil.
authors.forename: Kyria Santiago
authors.initials: KS
authors.lastname: Do Nascimento
authors.affiliation: BioMar, Laboratory of Marine Biotechnology, Department of Fishing Engineering, Federal University of Ceará, Fortaleza, CE 60020-181, Brazil.
authors.forename: Silvana
authors.initials: S
authors.lastname: Saker-Sampaio
authors.affiliation: Department of Animal Morphology and Physiology, Federal Rural University of Pernambuco, Recife, Pernambuco 52071-030, Brazil.
authors.forename: Valdemiro Amaro
authors.initials: VA
authors.lastname: Da Silva
authors.affiliation: Department of Animal Morphology and Physiology, Federal Rural University of Pernambuco, Recife, Pernambuco 52071-030, Brazil.
authors.forename: Ana Lúcia Figueiredo
authors.initials: ALF
authors.lastname: Porto
authors.affiliation: BioMol, Laboratory of Biologically Active Molecules, Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE 60020-181, Brazil.
authors.forename: Benildo Sousa
authors.initials: BS
authors.lastname: Cavada
authors.affiliation: BioMar, Laboratory of Marine Biotechnology, Department of Fishing Engineering, Federal University of Ceará, Fortaleza, CE 60020-181, Brazil.
authors.forename: Alexandre Holanda
authors.initials: AH
authors.lastname: Sampaio
authors.affiliation: LIBS, Integrated Laboratory of Biomolecules, Faculty of Medicine of Sobral, Federal University of Ceará, Fortaleza, CE 60020-181, Brazil.
authors.forename: Edson Holanda
authors.initials: EH
authors.lastname: Teixeira
authors.affiliation: BioMar, Laboratory of Marine Biotechnology, Department of Fishing Engineering, Federal University of Ceará, Fortaleza, CE 60020-181, Brazil.
authors.forename: Celso Shiniti
authors.initials: CS
authors.lastname: Nagano
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D020405:Dermis / Q000187:drug effects; D037102:Lectins / Q000737:chemistry* / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D020033:Protein Isoforms; D000461:Rhodophyta / Q000737:chemistry*; D020539:Sequence Analysis, Protein; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D037102:Lectins; D020033:Protein Isoforms
keywords: algal proteins; lectins; wound healing