title: Crystallization of uridine phosphorylase from Shewanella oneidensis MR-1 in the laboratory and under microgravity and preliminary X-ray diffraction analysis.
abstract: Uridine phosphorylase (UDP, EC 2.4.2.3), a key enzyme in the pyrimidine salvage pathway, catalyses the reversible phosphorolysis of uridine to uracil and ribose 1-phosphate. The gene expression of UDP from Shewanella oneidensis MR-1 was performed in the recipient strain Escherichia coli. The UDP protein was crystallized on earth (in the free form and in complex with uridine as the substrate) by the hanging-drop vapour-diffusion method at 296 K and under microgravity conditions (in the free form) aboard the Russian Segment of the International Space Station by the capillary counter-diffusion method. The data sets were collected to a resolution of 1.9 Å from crystals of the free form grown on earth, 1.6 Å from crystals of the complex with uridine and 0.95 Å from crystals of the free form grown under microgravity. All crystals belong to the space group P2(1) and have similar unit-cell parameters. The crystal of uridine phosphorylase grown under microgravity diffracted to ultra-high resolution and gave high-quality X-ray diffraction data.
authors.affiliation: Bach Institute of Biochemistry, Russian Academy of Sciences, 33 Leninskii Avenue, 119071 Moscow, Russian Federation. tn_safonova@inbi.ras.ru
authors.forename: Tatyana N
authors.initials: TN
authors.lastname: Safonova
authors.forename: Nadezhda N
authors.initials: NN
authors.lastname: Mordkovich
authors.forename: Konstantin M
authors.initials: KM
authors.lastname: Polyakov
authors.forename: Valentin A
authors.initials: VA
authors.lastname: Manuvera
authors.forename: Vladimir P
authors.initials: VP
authors.lastname: Veiko
authors.forename: Vladimir O
authors.initials: VO
authors.lastname: Popov
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D020592:Shewanella / Q000201:enzymology*; D014529:Uridine / Q000737:chemistry; D014543:Uridine Phosphorylase / Q000737:chemistry*; D014893:Weightlessness
chemical_list: D001426:Bacterial Proteins; D014543:Uridine Phosphorylase; D014529:Uridine