title: Phenolic glycosides as inhibitors of inducible nitric oxide synthase from Populus davidiana in LPS-activated RAW 264.7 murine macrophages.
abstract: Nitric oxide (NO) produced in large amounts by inducible nitric oxide synthase (i-NOS) is known to be responsible for the vasodilation and hypotension observed in septic shock and inflammation. Inhibitors of i-NOS, thus, may be useful candidates for the treatment of inflammatory diseases that accompany the overproduction of NO. Two phenolic glycosides, salicortin (1) and salicortin-6'-benzoate (2), were purified as active principles from the extracts of Populus davidiana by activity-guided purification procedures. They showed dose dependent inhibition of NO production in lipopolysaccharide (LPS)-activated RAW 264.7 cells. The IC50 values of salicortin (1) and salicortin-6'-benzoate (2) was 15 microM and over 50 microM, respectively. In immunoblot analysis, salicortin inhibited the expression of i-NOS. These new inhibitors of overproduction of NO may have potentials for the treatment of inflammation.
authors.affiliation: Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University, Seoul, Republic of Korea.
authors.forename: Hwa Jin
authors.initials: HJ
authors.lastname: Lee
authors.forename: Ji Sun
authors.initials: JS
authors.lastname: Kim
authors.forename: Young-Kyoon
authors.initials: YK
authors.lastname: Kim
authors.forename: Jae-Ha
authors.initials: JH
authors.lastname: Ryu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D006027:Glycosides / Q000494:pharmacology*; D007202:Indicators and Reagents; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors*; D010636:Phenols / Q000494:pharmacology*; D032107:Populus / Q000737:chemistry*; D012997:Solvents
chemical_list: D004791:Enzyme Inhibitors; D005960:Glucosides; D006027:Glycosides; D007202:Indicators and Reagents; D008070:Lipopolysaccharides; D010636:Phenols; D012997:Solvents; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C113068:salicortin