title: [Study of the invasiveness and tumour formation of Bcrp1(+) HeLa cervical cancer cells].
abstract: OBJECTIVE
  To make sure whether or not Bcrp1 is the marker of cervical cancer stem cells or not by studying the invasive ability and formation of tumors of Bcrp1(+) phenotype HeLa cells.
  
  
  METHODS
  The tumor cell migration and invasion assay were used by boyden chamber to identify the invasive ability of Bcrp1(+) phenotype HeLa cells. The formation of tumors in vivo experiments were completed, in which the two groups of cells with different concentrations were inoculated in non obese diabetes-severe combined immunodeficiency disease (NOD/SCID) mice (1×10(4), 1×10(5), 1×10(6)/ml), and the differences of time, rate and volume in the formation of tumors between two groups were observed.
  
  
  RESULTS
  (1) In the invasion assay, the amount of cells that invaded through the artificial basement membrane in Bcrp1(+) group were 99 ± 14, which was significantly greater than those in Bcrp1(-) group (57 ± 13, P < 0.05);the length of the Bcrp1(+) group was (366 ± 52) µm, which was significantly greater than the Bcrp1(-) group (301 ± 54) µm (P < 0.05). (2) Following transplantation of 1×10(4) cells, only the Bcrp1(+) cells formed tumors in NOD/SCID mice. When 1×10(5) or 1×10(6) cells were transplanted, the tumor incidence and the tumor mass were greater in the Bcrp1(+) groups than those in the Bcrp1(-) groups (P < 0.05).
  
  
  CONCLUSIONS
  Bcrp1(+) HeLa cell have the greater capacity of invasive and the tumorigenicity, which may contain cancer stem cells.
authors.affiliation: Department of Obstetric and Gynecology, Peking University People's Hospital, Beijing 100044, China.
authors.forename: Song-ling
authors.initials: SL
authors.lastname: Zhang
authors.forename: Xiao-wei
authors.initials: XW
authors.lastname: Yu
authors.forename: Jian-liu
authors.initials: JL
authors.lastname: Wang
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D000818:Animals; D002465:Cell Movement; D017077:Culture Media, Conditioned; D005260:Female; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*; D010641:Phenotype; D002583:Uterine Cervical Neoplasms / Q000473:pathology*
chemical_list: D018528:ATP-Binding Cassette Transporters; D017077:Culture Media, Conditioned