title: Effects of potassium apigenin and verbena extract on the wound healing process of SKH-1 mouse skin.
abstract: Tissue repair is a complex process, which may be favoured or inhibited by different factors. Potassium apigenin (AP) and other flavonoids present in verbena extract (PLX(®) ) possess powerful anti-inflammatory properties. The aim of this study was to evaluate the effects of topical treatment with AP and PLX gels on wounds inflicted on SKH-1/CRL mice. Forty-eight SKH-1 mice were used (4 groups with 12 animals each), which were subjected to wound excision with a round scalpel, 4 mm in diameter, on the dorsal skin. The animals were divided into four groups: Group I received topical applications of apigenin gel; Group II received PLX gel; Group III received vehicle gel; Group IV acted as control. Wound contraction, reepithelialisation, inflammation and neovascularisation (by means of immunohistochemical staining with anti-laminin) were recorded at study periods established at 2, 7 and 14 days. Reepithelialisation was faster in Groups I and II at 7 days (56·25% grade 3 and 43·75% grade 4) compared with the other groups. The degree of inflammation showed improvement with a tendency towards statistical significance in Groups I and II at 2 and 7 days. Anti-laminin staining was more intense in the group treated with PLX at the 2- and 7-day periods. Topical treatment with PLX gel improved the degree of reepithelialisation and inflammation, and favoured neo-vascularisation of the wounds at 2 and 7 days following surgery.
authors.affiliation: Oral Medicine Unit, Faculty of Medicine and Dentistry, University of Murcia, Spain.
authors.forename: Pia
authors.initials: P
authors.lastname: Lopez-Jornet
authors.forename: Fabio
authors.initials: F
authors.lastname: Camacho-Alonso
authors.forename: Francisco
authors.initials: F
authors.lastname: Gómez-Garcia
authors.forename: Francisco
authors.initials: F
authors.lastname: Molina Miñano
authors.forename: Xabier
authors.initials: X
authors.lastname: Cañas
authors.forename: Ana
authors.initials: A
authors.lastname: Serafín
authors.forename: Julian
authors.initials: J
authors.lastname: Castillo
authors.forename: Vicente
authors.initials: V
authors.lastname: Vicente-Ortega
mesh_terms: D000287:Administration, Topical; D000328:Adult; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D047310:Apigenin / Q000494:pharmacology*; D004195:Disease Models, Animal; D006801:Humans; D032411:Lippia; D008297:Male; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D011446:Prospective Studies; D011897:Random Allocation; D012867:Skin / Q000187:drug effects / Q000293:injuries; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy*
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D047310:Apigenin
keywords: Apigenin; Excision wound; Flavonoids; Mice; Skin