title: Pretreatment of Agave americana stalk for enzymatic saccharification.
abstract: Agave americana is one of commonly grown agave species but currently less valuable because its large flower stalk cannot be used for producing alcoholic beverage. In the present study, the stalk was pretreated with dilute acid (DA), sulfite (SPORL), and sodium hydroxide (NaOH) to preliminarily assess its potential as feedstock for bioethanol production. The changes of cell wall components during the pretreatments, enzymatic digestibility of the pretreated stalks, and the adsorption of cellulases on the substrates were investigated. Results indicated that the pretreatments significantly improved the enzymatic digestibility of the agave stalk. SPORL pretreatment gave higher substrate and sugar yields, while NaOH pretreated stalk had better digestibility under the investigated conditions. The better hydrolysability of NaOH-pretreated stalk was attributed to low lignin and hemicellulose content and high affinity to cellulases.
authors.affiliation: Department of Biological Systems Engineering, University of Wisconsin-Madison, 460 Henry Mall, Madison, WI 53706, USA.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yang
authors.forename: Xuejun
authors.initials: X
authors.lastname: Pan
mesh_terms: D027882:Agave / Q000737:chemistry*; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D006868:Hydrolysis / Q000187:drug effects; D012972:Sodium Hydroxide / Q000494:pharmacology*; D062611:Solid Waste; D013447:Sulfites / Q000494:pharmacology*; D013464:Sulfuric Acids / Q000494:pharmacology*; D013997:Time Factors; D014990:Xylans / Q000378:metabolism
chemical_list: D062611:Solid Waste; D013447:Sulfites; D013464:Sulfuric Acids; D014990:Xylans; D012972:Sodium Hydroxide; D002482:Cellulose; D002480:Cellulase; C033158:sulfuric acid; C025026:sodium sulfite