title: Passive circulating cell sorting by deformability using a microfluidic gradual filter.
abstract: The deformability of circulating leukocytes plays an important role in the physiopathology of several diseases like sepsis or acute respiratory distress syndrome (ARDS). We present here a microfluidic method for the passive testing, sorting and separating of non-adherent cell populations by deformability. It consists of microfluidic sieves in series with pore sizes decreasing from the upstream to the downstream. The method capabilities are demonstrated with monocytic cell lines (THP-1) treated by Jasplakinolide (a stabilizer of polymerized actin), LatrunculinA (an inhibitor of actin polymerization), and with the plasma of patients suffering from ARDS. Simple sample injection with standard syringes and pumps makes the method readily adapted for experimentation in hospitals.
authors.affiliation: Adhésion & Inflammation, INSERM U1067-CNRS UMR7333, and Université Aix-Marseille, Assistance Publique-Hôpitaux de Marseille, Case 937, 163 Avenue de Luminy, F-13009 Marseille, France.
authors.forename: P
authors.initials: P
authors.lastname: Preira
authors.forename: V
authors.initials: V
authors.lastname: Grandné
authors.forename: J-M
authors.initials: JM
authors.lastname: Forel
authors.forename: S
authors.initials: S
authors.lastname: Gabriele
authors.forename: M
authors.initials: M
authors.lastname: Camara
authors.forename: O
authors.initials: O
authors.lastname: Theodoly
mesh_terms: D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D002460:Cell Line; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods*; D002478:Cells, Cultured; D047630:Depsipeptides / Q000494:pharmacology; D005374:Filtration / Q000295:instrumentation*; D018056:Hemorheology; D006801:Humans; D007962:Leukocytes / Q000187:drug effects / Q000473:pathology; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D016062:Porosity; D012128:Respiratory Distress Syndrome / Q000097:blood / Q000503:physiopathology; D053778:Thiazolidines / Q000494:pharmacology
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D047630:Depsipeptides; D053778:Thiazolidines; C057531:jasplakinolide; C037067:latrunculin A