title: Effect of photobiomodulation on expression of IL-1β in skeletal muscle following acute injury.
abstract: Muscle repair is regulated by growth factors and cytokines. Low-level laser therapy (LLLT) seems to influence acute inflammation and accelerate skeletal muscle repair. This study verifies the effect of LLLT on the expression of IL-1β in the tibialis anterior (TA) muscle of rats following acute injury. Wistar rats (n=35) were allocated into three groups: control (without lesion and LLLT, n=5), injury group (n=15), and injury + LLLT group (n=15). The acute injury was induced by the contact with a cooled metal probe (3 mm in diameter) during 10 s, twice, in the same muscle area. LLLT was used three times a week using the InGaAlP laser (660 nm; beam spot of 0.04 cm(2), output power of 20 mW, power density of 500 mW/cm(2), and energy density of 5 J/cm(2) during 10 s). The animals were analyzed at 1, 7, and 14 days following injury. TA muscles samples were used for obtaining total RNA and performing cDNA synthesis. Real-time polymerase chain reactions were realized using IL-1β primer. There was a decrease in IL-1β expression after 7 days in LLLT group in comparison with the no treated group. In conclusion, LLLT was able to decrease IL-1β expression during the skeletal muscle repair following an acute injury.
authors.affiliation: Rehabilitation Sciences and Biophotonics, Universidade Nove de Julho-UNINOVE, São Paulo, São Paulo, Brazil. kristianneporta@gmail.com
authors.forename: Kristianne Porta Santos
authors.initials: KP
authors.lastname: Fernandes
authors.forename: Agnelo Neves
authors.initials: AN
authors.lastname: Alves
authors.forename: Fábio Daumas
authors.initials: FD
authors.lastname: Nunes
authors.forename: Nadhia Helena Costa
authors.initials: NH
authors.lastname: Souza
authors.forename: José Antônio
authors.initials: JA
authors.lastname: Silva
authors.forename: Sandra Kalil
authors.initials: SK
authors.lastname: Bussadori
authors.forename: Raquel Agnelli Mesquita
authors.initials: RA
authors.lastname: Ferrari
mesh_terms: D000818:Animals; D015870:Gene Expression / Q000528:radiation effects; D053583:Interleukin-1beta / Q000235:genetics*; D054023:Lasers, Semiconductor / Q000627:therapeutic use; D028022:Low-Level Light Therapy; D008297:Male; D018482:Muscle, Skeletal / Q000276:immunology* / Q000293:injuries / Q000528:radiation effects*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000235:genetics / Q000276:immunology / Q000528:radiation effects
chemical_list: D053583:Interleukin-1beta; D012333:RNA, Messenger