title: In vitro biocompatibility and antimicrobial activity of poly(ε-caprolactone)/montmorillonite nanocomposites.
abstract: A triblock copolymer based on poly(ε-caprolactone) (PCL) and 2-(N,N-diethylamino)ethyl methacrylate (DEAEMA)/2-(methyl-7-nitrobenzofurazan)amino ethyl acrylate (NBD-NAcri), was synthesized via atom transfer radical polymerization (ATRP). The corresponding chlorohydrated copolymer, named as PCL-b-DEAEMA, was prepared and anchored via cationic exchange on montmorillonite (MMT) surface. (PCL)/layered silicate nanocomposites were prepared through melt intercalation, and XRD and TEM analysis showed an exfoliated/intercalated morphology for organomodified clay. The surface characterization of the nanocomposites was undertaken by using contact angle and AFM. An increase in the contact angle was observed in the PCL/MMT(PCL-b-DEAEMA) nanocomposites with respect to PCL. The AFM analysis showed that the surface of the nanocomposites became rougher with respect to the PCL when MMTk10 or MMT(PCL-b-DEAEMA) was incorporated, and the value increased with the clay content. The antimicrobial activity of the nanocomposites against B. subtilis and P. putida was tested. It is remarkable that the biodegradation of PCL/MMT(PCL-b-DEAEMA) nanocomposites, monitored by the production of carbon dioxide and by chemiluminescence emission, was inhibited or retarded with respect to the PCL and PCL/1-MMTk10. It would indicate that nature of organomodifier in the clay play an important role in B. subtilis and P. putida adhesion processes. Biocompatibility studies demonstrate that both PCL and PCL/MMT materials allow the culture of murine L929 fibroblasts on its surface with high viability, very low apoptosis, and without plasma membrane damage, making these materials very adequate for tissue engineering.
authors.affiliation: Polymer Photochemistry Group, Instituto de Ciencia y Tecnología de Polímeros, C.S.I.C. Juan de la Cierva 3, 28006 Madrid, Spain. tcorrales@ictp.csic.es
authors.forename: T
authors.initials: T
authors.lastname: Corrales
authors.forename: I
authors.initials: I
authors.lastname: Larraza
authors.forename: F
authors.initials: F
authors.lastname: Catalina
authors.forename: T
authors.initials: T
authors.lastname: Portolés
authors.forename: C
authors.initials: C
authors.lastname: Ramírez-Santillán
authors.forename: M
authors.initials: M
authors.lastname: Matesanz
authors.forename: C
authors.initials: C
authors.lastname: Abrusci
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D017209:Apoptosis; D001412:Bacillus subtilis / Q000187:drug effects; D001546:Bentonite / Q000138:chemical synthesis* / Q000494:pharmacology; D001672:Biocompatible Materials / Q000138:chemical synthesis*; D002152:Calorimetry, Differential Scanning; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D005434:Flow Cytometry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051379:Mice; D053761:Nanocomposites / Q000737:chemistry*; D011091:Polyesters / Q000138:chemical synthesis* / Q000494:pharmacology; D016958:Pseudomonas putida / Q000187:drug effects; D017640:Silicates / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D011091:Polyesters; D017640:Silicates; D001546:Bentonite; C016240:polycaprolactone; D007770:L-Lactate Dehydrogenase