title: On the origins and development of the (32)P-postlabelling assay for carcinogen-DNA adducts.
abstract: The (32)P-postlabelling method for the analysis of carcinogen-DNA adducts originated 30years ago from Baylor College of Medicine in Houston and was the work of a team comprised of Kurt and Erica Randerath, Ramesh Gupta and Vijay Reddy. With subsequent modifications and developments, it has become a highly sensitive and versatile method for the detection of DNA adducts that has been applied in a wide range of human, animal and in vitro studies. These include monitoring human exposure to environmental and occupational carcinogens, investigating genotoxicity of chemicals, elucidating pathways of metabolic activation of carcinogens, mechanistic studies of DNA repair, analysing the genotoxicity of complex mixtures and in ecotoxicology studies. Its use has been instrumental in providing new clues to the aetiology of some cancers and in identifying a new human carcinogen.
authors.affiliation: King's College London, Analytical and Environmental Sciences Division, MRC-HPA Centre for Environment and Health, Franklin-Wilkins Building, 150 Stamford Street, London SE1 9NH, UK. Electronic address: david.phillips@kcl.ac.uk.
authors.forename: David H
authors.initials: DH
authors.lastname: Phillips
mesh_terms: D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D018736:DNA Adducts / Q000032:analysis*; D004781:Environmental Exposure / Q000032:analysis*; D006801:Humans; D007553:Isotope Labeling / Q000379:methods* / Q000592:standards; D010761:Phosphorus Radioisotopes; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D002273:Carcinogens; D018736:DNA Adducts; D010761:Phosphorus Radioisotopes