title: Characterization and antitumor activities of a polysaccharide from the rhizoma of Menispermum dauricum.
abstract: The rhizome of Menispermum dauricum DC (Menispermaceae) is one of the most commonly used traditional Chinese medicines officially listed in Chinese Pharmacopeia. In present study, we purified a water-soluble polysaccharide (WMDP) from this plant and investigated its physicochemical properties. WMDP was a homogeneous polysaccharide, with an average molecular weight of approximately 3.5×10(4)Da, as determined by high-performance gel-permeation chromatography (HPGPC). Gas chromatography (GC) analysis identified that WMDP was composed of Glc, Gal, Xyl, Rha, Ara and Man in the ratio of 2.45:2.13:1.05:1.29:1.63:1.45. The interreaction between Gongo Red and WMDP in NaOH solutions resulted in the shift of maximum absorption, indicating WMDP had a triple-helix conformation. We also investigated the antitumor activities and mechanisms of WMDP in human ovarian carcinoma SKOV3 cells. The experimental evidence showed that WMDP significantly inhibited cell proliferation and DNA synthesis in SKOV3 cells in a concentration-dependent manner, due to a significant increase in the number of apoptotic cells. Furthermore, treatment with WMDP caused a rapid loss of intracellular glutathione (GSH) content and stimulation of reactive oxygen species (ROS). In addition, nuclear factor-kappa B (NF-κB) in SKOV3 cells received WMDP treatment was inactivated. Taken together, induction of apoptosis on SKOV3 cells by WMDP was mainly associated with ROS production, GSH depletion and NF-κB inactivation.
authors.affiliation: Department of Gynecology, The Third Affiliated Hospital of Harbin Medical University, Harbin 150081, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Lin
authors.forename: Bairong
authors.initials: B
authors.lastname: Xia
authors.forename: Meng
authors.initials: M
authors.lastname: Yang
authors.forename: Shu
authors.initials: S
authors.lastname: Gao
authors.forename: Yanqiu
authors.initials: Y
authors.lastname: Huo
authors.forename: Ge
authors.initials: G
authors.lastname: Lou
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002236:Carbohydrate Conformation; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D031603:Menispermum / Q000737:chemistry*; D016328:NF-kappa B / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011485:Protein Binding; D017382:Reactive Oxygen Species / Q000378:metabolism; D027343:Rhizome / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D011134:Polysaccharides; D017382:Reactive Oxygen Species; D005978:Glutathione