title: Dynamics of fungal colonization in a new medical mycology laboratory.
abstract: OBJECTIVE
  Study of the spatio-temporal fungal colonization in a new medical mycology laboratory.
  
  
  METHODS
  A 17-month survey of airborne fungal contamination was conducted in a new medical mycology laboratory at a tertiary care university hospital. This survey was implemented at three different periods: before the new premises were occupied (period A), during the move into the new laboratory (period B) and after resumption of the mycological activities in these new premises (period C).
  
  
  RESULTS
  During period A, the airborne fungal load ranged from 2.3 to 6 cfu/m(3). The most frequently recovered airborne fungi were Penicillium spp. (75 to 100%). During period B, a dramatic increase in Penicillium chrysogenum conidia was observed in the air of the new laboratory (40 to 160 cfu/m(3)). During period C, the fungal load ranged from 4.5 to 8.4 cfu/m(3). Penicillium was the most common genus identified in rooms of the laboratory where no filamentous fungi were handled, while Aspergillus was clearly the predominant genus (78%) in the room dedicated to the culture of filamentous fungi.
  
  
  CONCLUSIONS
  We suggest that the specific fungal ecology in air of the room dedicated to the culture of filamentous fungi is due to the handling of a large number of medical strains of A. fumigatus.
authors.affiliation: Laboratoire de parasitologie-mycologie, plateau technique de biologie du CHU, 2, rue Angélique-Ducoudray, BP 37013, 21070 Dijon cedex, France. marc.sautour@u-bourgogne.fr
authors.forename: M
authors.initials: M
authors.lastname: Sautour
authors.forename: I
authors.initials: I
authors.lastname: Fournel
authors.forename: F
authors.initials: F
authors.lastname: Dalle
authors.forename: C
authors.initials: C
authors.lastname: Calinon
authors.forename: C
authors.initials: C
authors.lastname: L'Ollivier
authors.forename: M
authors.initials: M
authors.lastname: Goyer
authors.forename: C
authors.initials: C
authors.lastname: Cachia
authors.forename: S
authors.initials: S
authors.lastname: Aho
authors.forename: N
authors.initials: N
authors.lastname: Sixt
authors.forename: O
authors.initials: O
authors.lastname: Vagner
authors.forename: B
authors.initials: B
authors.lastname: Cuisenier
authors.forename: A
authors.initials: A
authors.lastname: Bonnin
mesh_terms: D000391:Air Microbiology; D001230:Aspergillus / Q000254:growth & development / Q000302:isolation & purification; D015169:Colony Count, Microbial; D004784:Environmental Monitoring; D005658:Fungi / Q000254:growth & development*; D006757:Hospital Units; D006801:Humans; D007755:Laboratories, Hospital / Q000592:standards; D009172:Mycology / Q000592:standards; D010407:Penicillium / Q000254:growth & development / Q000302:isolation & purification