title: [Relation of -55CT polymorphism of UCP3 gene with weight loss and metabolic changes after a high polyunsaturated fat diet in obese patients].
abstract: OBJECTIVE
  The alteration in the protein expression of UCP3 could reduce energy consumption and increase energy storage as fat. The aim of our study was to investigate the influence of -55CT polymorphism of UCP3 gene in the metabolic response, weight loss and serum levels of adipokines following a hypocaloric diet rich in polyunsaturated fat in obese patients.
  
  
  METHODS
  A sample of 133 obese patients were analyzed prospectively for 3 months. The hypocaloric diet was 1459 kcal, 45.7% carbohydrate, 34.4% from 19.9% lipids and proteins. The fat distribution was, a 21.8% saturated fat, 55.5% monounsaturated and 22.7% of polyunsaturated fat (7 g per day of fatty acids w6, 2 g per day of w -3 and a ratio w6/w3 of 3.5).
  
  
  RESULTS
  A total of 100 patients (28 males/72 females) (75.2%) had genotype - 55CC (wild genotype group) and 33 patients (8 males/25 females) (24.8%) -55CT genotype (group mutant genotype). In the wild genotype, body mass index (-2.5 ± 5.3 kg/m²), weight (-4.2 ± 3.7 kg), fat mass (-3,7 ± 3.3 kg), waist circumference (-4.1 ± 2.9 cm), systolic blood pressure (-4.9 ± 10.1 mmHg), total cholesterol levels (- 16.1 ± 23.6 mg / dl), LDL cholesterol (-11.1 ± 26.8 mg/dl), triglycerides (-12.0 ± 46.8 mg/dl), insulin (-1.8 ± 4.5 IU/L), HOMA-R (-0.6 ± 1.5) and leptin (-6.2 ± 8.4 ng/ml) decreased. In the mutant genotype anthropometric parameters were significantly decreased without significant changes in biochemical parameters.
  
  
  CONCLUSIONS
  The T allele carriers of -55CT UCP3 polymorphism exhibit no metabolic response to weight loss induced by a hypocaloric diet rich in polyunsaturated fatty acids.
authors.affiliation: Centro de Investigación de Endocrinología y Nutrición Clínica, Facultad de Medicina, Hospital Rio Hortega, Universidad de Valladolid, Valladolid, España. dadluis@yahoo.es
authors.forename: D A
authors.initials: DA
authors.lastname: de Luis
authors.forename: R
authors.initials: R
authors.lastname: Aller
authors.forename: O
authors.initials: O
authors.lastname: Izaola
authors.forename: M
authors.initials: M
authors.lastname: GonzÁlez Sagrado
authors.forename: R
authors.initials: R
authors.lastname: Conde
authors.forename: M
authors.initials: M
authors.lastname: Ruiz Mambrilla
mesh_terms: D054392:Adipokines / Q000097:blood; D000328:Adult; D000483:Alleles; D015992:Body Mass Index; D005231:Fatty Acids, Unsaturated / Q000627:therapeutic use*; D005260:Female; D005838:Genotype; D006579:Heterozygote; D006801:Humans; D007473:Ion Channels / Q000235:genetics*; D008297:Male; D024101:Mitochondrial Proteins / Q000235:genetics*; D009765:Obesity / Q000178:diet therapy*; D011110:Polymorphism, Genetic / Q000235:genetics; D011446:Prospective Studies; D000071247:Uncoupling Protein 3; D015431:Weight Loss / Q000502:physiology*
chemical_list: D054392:Adipokines; D005231:Fatty Acids, Unsaturated; D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604392:UCP3 protein, human; D000071247:Uncoupling Protein 3