title: Enzymatically-gelled amylopectin-based substrates enable on-demand harvesting cells with preserving cell-to-cell connection using saliva.
abstract: The possibility of on-demand harvesting of cells using human saliva was investigated for amylopectin-based hydrogel substrate obtained through enzymatic reaction. The human epithelial cells grown on the surface of the hydrogels detached within 10 min with preserving cell-to-cell connection by soaking in the medium containing human saliva at 5% (v/v).
authors.affiliation: Department of Materials Science and Engineering, Osaka University, Toyonaka, Osaka, Japan. sakai@cheng.es.osaka-u.ac.jp
authors.forename: Shinji
authors.initials: S
authors.lastname: Sakai
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Masahito
authors.initials: M
authors.lastname: Taya
mesh_terms: D000681:Amylases / Q000378:metabolism; D000687:Amylopectin / Q000737:chemistry / Q000378:metabolism*; D018929:Cell Culture Techniques; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D020100:Hydrogels; D008297:Male; D012463:Saliva / Q000201:enzymology*; D023822:Tissue Engineering; D055815:Young Adult
chemical_list: D020100:Hydrogels; D000687:Amylopectin; D000681:Amylases