title: DsRed-mediated oligomerization stabilizes HMGB1 on chromatin in vivo and on DNA in vitro.
abstract: High-mobility group box-1 (HMGB1) is remarkably mobile in living cells, which reflects its ability to interact only transiently with both DNA and protein. This property is likely essential for HMGB1 nuclear activities. Nonetheless the weak interaction of HMGB1 with DNA and/or protein partners has also been a major limitation for investigating HMGB1 subnuclear localisation and for the identification of HMGB1 containing complexes by conventional biochemical approaches. In the present study, FRAP experiments demonstrated that DsRed-mediated oligomerization strongly reduces HMGB1 mobility due to an increased affinity for cellular chromatin. Moreover, oligomerized DsRed-HMGB1 exhibited a higher affinity for supercoiled DNA in vitro compared to its monomeric counterpart. These results indicate that DsRed-meditated oligomerization is prone to stabilize labile interactions involving HMGB1 both in vivo and in vitro.
authors.affiliation: Centre de Recherche des Cordeliers, Université Pierre et Marie Curie, Université Paris Descartes, INSERM, UMRS 872, Paris F-75006, France.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Messmer
authors.forename: Christophe
authors.initials: C
authors.lastname: Klein
authors.forename: Rachel
authors.initials: R
authors.lastname: Boniface
authors.forename: Nina F
authors.initials: NF
authors.lastname: Gnädig
authors.forename: Maxime
authors.initials: M
authors.lastname: Lecerf
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Barnay-Verdier
authors.forename: Vincent
authors.initials: V
authors.lastname: Maréchal
mesh_terms: D002843:Chromatin / Q000378:metabolism*; D004278:DNA, Superhelical / Q000378:metabolism*; D024243:HMGB1 Protein / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008164:Luminescent Proteins / Q000378:metabolism*; D055503:Protein Multimerization; D055550:Protein Stability; D020836:Protein Structure, Quaternary
chemical_list: D002843:Chromatin; D004278:DNA, Superhelical; D024243:HMGB1 Protein; D008164:Luminescent Proteins; C413663:fluorescent protein 583