title: Effect of fibrin-binding synthetic oligopeptide on the healing of full-thickness skin wounds in streptozotocin-induced diabetic rats.
abstract: The aim of this study was to investigate whether topical application of fibrin-binding oligopeptides derived from FN promotes wound healing in streptozotocin (STZ)-induced diabetic rats. Oligopeptides including fibrin-binding sequences (FF3: CFDKYTGNTYRV, FF5 : CTSRNRCNDQ) of FN repeats were synthesized. Each peptide was loaded in 15 x 15 mm fibrous alginate dressings, and the release kinetics of the peptides was evaluated using trinitrobenzene sulfonic acid for 336 hours. Two full-thickness cutaneous wounds were prepared on the dorsal skin of each 75 diabetes induced rats. Each wound was divided into FF3-loaded alginate dressing group, FF5-loaded alginate dressing group, alginate dressing group and negative control group. Animals were sacrificed at day 0,3,7 and 14. The wound closure rate, inflammation degree, expression of TGF-β1 and hydroxyproline contents were evaluated. Both FF3 and FF5 peptides were released rapidly within the first 24 hours. FF3-loaded dressing treated wounds closed significantly faster than other wounds at day 3. And at day 14, FF3- & FF5- loaded dressing treated wounds demonstrated less inflammatory cells infiltration than alginate dressing treated and negative group wounds. TGF-β1 positive cells were more abundant in FF3-, FF5-treated alginate dressing treated wound at day 3 and 14. At last, the hyrdroxyproline contents in the FF3, FF5 group were higher at day 7 and day 14. Topical application of fibrin-binding domain synthetic oligopeptides from FN resulted in acceleration of full-thickness cutaneous wound healing in diabetic rats.
authors.affiliation: Department of Periodontology, School of Dentistry, Dental Research Institute, Seoul National University, Seou1 110-768, Republic of Korea.
authors.forename: Jae-Eun
authors.initials: JE
authors.lastname: Chung
authors.forename: Yun-Jeong
authors.initials: YJ
authors.lastname: Kim
authors.forename: Yoon-Jeong
authors.initials: YJ
authors.lastname: Park
authors.forename: Ki-Tae
authors.initials: KT
authors.lastname: Koo
authors.forename: Yang-Jo
authors.initials: YJ
authors.lastname: Seol
authors.forename: Yong-Moo
authors.initials: YM
authors.lastname: Lee
authors.forename: In-Chul
authors.initials: IC
authors.lastname: Rhyu
authors.forename: Young
authors.initials: Y
authors.lastname: Ku
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D005337:Fibrin / Q000378:metabolism*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D011485:Protein Binding; D051381:Rats; D012867:Skin / Q000187:drug effects*; D013311:Streptozocin; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D009842:Oligopeptides; D013311:Streptozocin; D005337:Fibrin