title: [Protein-protein interaction network of receptor activator of nuclear factor-kappaB ligand and macrophage colony-stimulating factor induced differentiation of osteoclasts].
abstract: OBJECTIVE
  To systemically investigate receptor activator of nuclear factor-kappaB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) induced differentiation of osteoclasts.
  
  
  METHODS
  Mouse protein-protein interaction(PPI) database NIA and published microarray dataset GES16749 were used to construct and analyze PPI network of RANKL and M-CSF induced mouse monocyte RAW264.7.
  
  
  RESULTS
  In the PPI network, transforming growth factor beta receptor 1 (TGFBR1), Rous sarcoma oncogene (SRC), myelocytomatosis oncogene(MYC) and integrin beta 3 (ITGB3) were able to interact with more proteins and they were the key nodes in the signaling transduction.
  
  
  CONCLUSIONS
  TGFBR1, SRC, MYC and ITGB3 might be the key points of RANKL and M-CSF induced differentiation of osteoclasts.
authors.affiliation: Dept. of Oral Pathology, Stomatology Hospital, Zhejiang University College of Medicine, Hangzhou 310006, China.
authors.forename: Pingxiu
authors.initials: P
authors.lastname: Zhou
authors.forename: Ji'an
authors.initials: J
authors.lastname: Hu
authors.forename: Xiangyong
authors.initials: X
authors.lastname: Meng
mesh_terms: D000818:Animals; D002352:Carrier Proteins; D002454:Cell Differentiation; D016173:Macrophage Colony-Stimulating Factor; D008562:Membrane Glycoproteins; D051379:Mice; D010010:Osteoclasts; D060066:Protein Interaction Maps; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B
chemical_list: D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D016173:Macrophage Colony-Stimulating Factor