title: Induction of endometriotic nodules in an experimental baboon model mimicking human deep nodular lesions.
abstract: OBJECTIVE
  To establish an experimental model for the study of deep nodular endometriosis.
  
  
  METHODS
  Induction of nodular endometriosis in baboons by grafting different uterine specimens to the peritoneal cavity.
  
  
  METHODS
  Research and university facilities.
  
  
  METHODS
  Ten baboons, to develop a model of induced deep nodular endometriosis.
  
  
  METHODS
  Biopsies of endometrium, and endometrium plus the junctional zone (JZ), full uterine thickness, and myometrium grafted to the peritoneum.
  
  
  METHODS
  Macroscopic descriptions recorded for observed induced lesions; staining with hematoxylin and eosin for histological evaluation and specific antibodies (CK22, CD10) for immunohistochemical studies; and analysis of surface area and volume of lesions, glandular density, and invasion of surrounding organs.
  
  
  RESULTS
  The incidence of induced nodular endometriosis was 100%, but the extent depended on the tissue grafted. Lesions induced after grafting specimens containing the JZ were statistically significantly larger than those not containing the JZ. Surrounding organ invasion was reported in more than 40% of lesions after grafting specimens containing the JZ.
  
  
  CONCLUSIONS
  The first experimental model of nodular endometriosis allows investigation of deeper nodular lesions as well as invasion phenomena associated with nodular lesions.
authors.affiliation: Department of Gynecology, Medical School, Institute of Experimental and Clinical Research, Université Catholique de Louvain, Brussels, Belgium. jacques.donnez@uclouvain.be
authors.forename: Olivier
authors.initials: O
authors.lastname: Donnez
authors.forename: Anne
authors.initials: A
authors.lastname: Van Langendonckt
authors.forename: Sylvie
authors.initials: S
authors.lastname: Defrère
authors.forename: Sébastien
authors.initials: S
authors.lastname: Colette
authors.forename: Olivier
authors.initials: O
authors.lastname: Van Kerk
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Dehoux
authors.forename: Jean
authors.initials: J
authors.lastname: Squifflet
authors.forename: Jacques
authors.initials: J
authors.lastname: Donnez
mesh_terms: D000818:Animals; D001706:Biopsy; D004195:Disease Models, Animal; D004715:Endometriosis / Q000473:pathology* / Q000503:physiopathology*; D005260:Female; D010535:Laparoscopy; D009215:Myometrium / Q000473:pathology / Q000637:transplantation; D048530:Papio anubis; D010529:Peritoneal Cavity / Q000473:pathology / Q000601:surgery; D000267:Tissue Adhesions / Q000473:pathology / Q000503:physiopathology