title: Comparative functional cell biological analysis of mesenchymal stem cells of the head and neck region: potential impact on wound healing, trauma, and infection.
abstract: BACKGROUND
  Mesenchymal stem cells (MSCs) are multipotent mesenchymal progenitor cells, originally identified in bone-marrow. Little is known about MSCs of the head and neck region. We investigated cell biological properties with a potential impact on wound healing of 2 different tissue-resident MSC populations.
  
  
  METHODS
  MSCs were isolated from human nasal mucosa (nmMSCs) and parotid gland (pgMSCs). Clonogenic potential, cell surface markers, cytokine secretion, chemokine receptor expression, mobility, and adhesion to extracellular matrix were examined in unstimulated and stimulated MSCs.
  
  
  RESULTS
  NmMSCs had the higher clonogenic potential. PgMSCs showed a broader panel of chemokine receptor expression and displayed higher mobility, especially after challenge with bacterial lipopolysaccharide (LPS). NmMSCs were less mobile and showed increased LPS-induced secretion of the inflammatory cytokine interleukin-8 (IL-8) compared with pgMSCs.
  
  
  CONCLUSIONS
  These data highlight functional differences between tissue-resident MSCs of the head and neck region, which may impact functional properties of these cells in response to trauma or infection.
authors.affiliation: Department of Otorhinolaryngology, University Hospital Bonn, Bonn, Germany.
authors.forename: Mark
authors.initials: M
authors.lastname: Jakob
authors.forename: Hatim
authors.initials: H
authors.lastname: Hemeda
authors.forename: Kirsten
authors.initials: K
authors.lastname: Bruderek
authors.forename: Andreas O
authors.initials: AO
authors.lastname: H Gerstner
authors.forename: Friedrich
authors.initials: F
authors.lastname: Bootz
authors.forename: Stephan
authors.initials: S
authors.lastname: Lang
authors.forename: Sven
authors.initials: S
authors.lastname: Brandau
mesh_terms: D001424:Bacterial Infections / Q000503:physiopathology; D015415:Biomarkers / Q000097:blood; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006257:Head; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000473:pathology*; D009297:Nasal Mucosa / Q000166:cytology; D009333:Neck; D010306:Parotid Gland / Q000166:cytology; D012038:Regeneration / Q000502:physiology*; D014945:Wound Healing / Q000502:physiology*; D014947:Wounds and Injuries / Q000473:pathology* / Q000503:physiopathology
chemical_list: D015415:Biomarkers; D016207:Cytokines