title: Lonsdalea quercina subsp. populi subsp. nov., isolated from bark canker of poplar trees.
abstract: Seven Gram-negative bacterial strains were isolated from oozing bark canker of poplar (Populus × euramericana) trees in Hungary. They showed high (>98.3%) 16S rRNA gene sequence similarity to Lonsdalea quercina; however, they differed from this species in several phenotypic characteristics. Multilocus sequence analysis based on three housekeeping genes (gyrB, atpD and infB) revealed, and DNA-DNA hybridization analysis confirmed, that this group of bacterial strains forms a distinct lineage within the species Lonsdalea quercina. A detailed study of phenotypic and physiological characteristics confirmed the separation of isolates from poplars from other subspecies of L. quercina; therefore, a novel subspecies, Lonsdalea quercina subsp. populi, type strain NY060(T) (=DSM 25466(T)=NCAIM B 02483(T)), is proposed.
authors.affiliation: Research and Extension Centre for Fruit Growing, Vadastag 2, H-4244 Újfehértó, Hungary.
authors.forename: Tímea
authors.initials: T
authors.lastname: Tóth
authors.affiliation: Nemaform Ltd, Jókai Mór 37, H-4320 Nagykálló, Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Lakatos
authors.affiliation: Hungarian Forest Research Institute, Hegyalja 18, H-3232 Mátrafüred, Hungary.
authors.forename: András
authors.initials: A
authors.lastname: Koltay
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D004755:Enterobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D006814:Hungary; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D032107:Populus / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S