title: A new F-actin structure in fungi: actin ring formation around the cell nucleus of Cryptococcus neoformans.
abstract: The F-actin cytoskeleton of Cryptococcus neoformans is known to comprise actin cables, cortical patches and cytokinetic ring. Here, we describe a new F-actin structure in fungi, a perinuclear F-actin collar ring around the cell nucleus, by fluorescent microscopic imaging of rhodamine phalloidin-stained F-actin. Perinuclear F-actin rings form in Cryptococcus neoformans treated with the microtubule inhibitor Nocodazole or with the drug solvent dimethyl sulfoxide (DMSO) or grown in yeast extract peptone dextrose (YEPD) medium, but they are absent in cells treated with Latrunculin A. Perinuclear F-actin rings may function as 'funicular cabin' for the cell nucleus, and actin cables as intracellular 'funicular' suspending nucleus in the central position in the cell and moving nucleus along the polarity axis along actin cables.
authors.affiliation: Department of Biology, Faculty of Medicine, Masaryk University, Brno 62500, Czech Republic. mkopecka@med.muni.cz
authors.forename: Marie
authors.initials: M
authors.lastname: Kopecká
authors.forename: Susumu
authors.initials: S
authors.lastname: Kawamoto
authors.forename: Masashi
authors.initials: M
authors.lastname: Yamaguchi
mesh_terms: D008841:Actin Cytoskeleton / Q000648:ultrastructure*; D000199:Actins / Q000032:analysis*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D002467:Cell Nucleus / Q000648:ultrastructure*; D003455:Cryptococcus neoformans / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology; D008387:Marine Toxins / Q000494:pharmacology; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000187:drug effects; D015739:Nocodazole / Q000494:pharmacology; D010590:Phalloidine / Q000031:analogs & derivatives; D012235:Rhodamines; D053778:Thiazolidines / Q000494:pharmacology; D050257:Tubulin Modulators / Q000494:pharmacology
chemical_list: D000199:Actins; D019086:Bridged Bicyclo Compounds, Heterocyclic; D016166:Free Radical Scavengers; D008387:Marine Toxins; D012235:Rhodamines; D053778:Thiazolidines; D050257:Tubulin Modulators; C504731:rhodamine-phalloidin; D010590:Phalloidine; D015739:Nocodazole; C037067:latrunculin A; D004121:Dimethyl Sulfoxide