title: Supporting conditional mouse mutagenesis with a comprehensive cre characterization resource.
abstract: Full realization of the value of the loxP-flanked alleles generated by the International Knockout Mouse Consortium will require a large set of well-characterized cre-driver lines. However, many cre driver lines display excision activity beyond the intended tissue or cell type, and these data are frequently unavailable to the potential user. Here we describe a high-throughput pipeline to extend characterization of cre driver lines to document excision activity in a wide range of tissues at multiple time points and disseminate these data to the scientific community. Our results show that the majority of cre strains exhibit some degree of unreported recombinase activity. In addition, we observe frequent mosaicism, inconsistent activity and parent-of-origin effects. Together, these results highlight the importance of deep characterization of cre strains, and provide the scientific community with a critical resource for cre strain information.
authors.affiliation: The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609, USA.
authors.forename: Caleb S
authors.initials: CS
authors.lastname: Heffner
authors.forename: C
authors.initials: C
authors.lastname: Herbert Pratt
authors.forename: Randal P
authors.initials: RP
authors.lastname: Babiuk
authors.forename: Yashoda
authors.initials: Y
authors.lastname: Sharma
authors.forename: Stephen F
authors.initials: SF
authors.lastname: Rockwood
authors.forename: Leah R
authors.initials: LR
authors.lastname: Donahue
authors.forename: Janan T
authors.initials: JT
authors.lastname: Eppig
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Murray
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism; D005260:Female; D017930:Genes, Reporter; D040582:Inheritance Patterns / Q000235:genetics; D019426:Integrases / Q000378:metabolism*; D008297:Male; D051379:Mice; D016296:Mutagenesis / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics; D015203:Reproducibility of Results; D019076:Transgenes / Q000235:genetics
chemical_list: C493411:Fabp4 protein, mouse; D050556:Fatty Acid-Binding Proteins; C045073:Cre recombinase; D019426:Integrases