title: microRNA-195 suppresses osteosarcoma cell invasion and migration in vitro by targeting FASN.
abstract: microRNAs are involved in different cancer-related processes. miR-195, one of the miR-16/15/195/424/497 family members, has been shown to act as a tumor suppressor during tumorigenesis. However, the function of miR-195 in osteosarcoma is still unclear. In our study, the miR-195 expression level was upregulated in osteosarcoma cells, by transfection with miR-195, and the fatty acid synthase (FASN) mRNA and protein expression levels were measured by RT-PCR and western blotting. Cell migration and invasion was measured using wound healing migration and Transwell invasion assays. We found that the upregulation of miR-195 greatly decreased cell invasion and the migration of U2OS. We also identified that FASN may be a direct target of miR-195 by the luciferase activity assay. These findings provide evidence that miR-195 plays a key role in inhibiting osteosarcoma cell migration and invasion through targeting FASN, and strongly suggest that exogenous miR-195 may have therapeutic value in treating osteosarcoma.
authors.affiliation: Department of Orthopedics, First Affiliated Hospital of Nanchang University;
authors.forename: Jian Hua
authors.initials: JH
authors.lastname: Mao
authors.forename: Rong Ping
authors.initials: RP
authors.lastname: Zhou
authors.forename: Ai Fen
authors.initials: AF
authors.lastname: Peng
authors.forename: Zhi Li
authors.initials: ZL
authors.lastname: Liu
authors.forename: Shan Hu
authors.initials: SH
authors.lastname: Huang
authors.forename: Xin Hua
authors.initials: XH
authors.lastname: Long
authors.forename: Yong
authors.initials: Y
authors.lastname: Shu