title: Methadone diminishes neuroinflammation and disease severity in EAE through modulating T cell function.
abstract: Methadone is known to exert modulatory effects on the immune system. We investigated the potential effects of methadone on infiltration of inflammatory cells into the spinal cord, as well as the proliferative and cytokine responses of T cells in MOG(35-55)-induced experimental autoimmune encephalomyelitis in mice. Methadone significantly suppressed clinical signs of the disease and level of inflammatory cytokines (p<0.05) produced by T cells. Moreover, invasion of inflammatory cells into the spinal cord was significantly decreased by methadone (p<0.05). Our data point to therapeutic effects of methadone and highlight the beneficial role of opioid receptor signaling in the context of autoimmune neuroinflammation.
authors.affiliation: Pathobiology Department, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran; Shefa Neuroscience Research Center, Tehran, Iran. kafami@sina.tums.ac.ir
authors.forename: Laya
authors.initials: L
authors.lastname: Kafami
authors.forename: Ifa
authors.initials: I
authors.lastname: Etesami
authors.forename: Mina
authors.initials: M
authors.lastname: Felfeli
authors.forename: Neda
authors.initials: N
authors.lastname: Enayati
authors.forename: Roya
authors.initials: R
authors.lastname: Ghiaghi
authors.forename: Atefeh
authors.initials: A
authors.lastname: Aminian
authors.forename: Ahmadreza
authors.initials: A
authors.lastname: Dehpour
mesh_terms: D000701:Analgesics, Opioid / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy* / Q000276:immunology; D005260:Female; D007155:Immunologic Factors / Q000494:pharmacology; D008691:Methadone / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011897:Random Allocation; D012720:Severity of Illness Index; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism
chemical_list: D000701:Analgesics, Opioid; D007155:Immunologic Factors; D008691:Methadone