title: Biodegradation of ivory (natural apatite): possible involvement of fungal activity in biodeterioration of the Lewis Chessmen.
abstract: Fungal biodeterioration of ivory was investigated with in vitro inoculation of samples obtained from boar and walrus tusks with the fungi Aspergillus niger and Serpula himantioides, species of known geoactive abilities. A combination of light and scanning electron microscopy together with associated analytical techniques was used to characterize fungal interactions with the ivory, including changes in ivory composition, dissolution and tunnelling, and the formation of new biominerals. The research was aimed at providing further understanding of the potential roles of fungi in the colonization and deterioration of ivory in terrestrial environments, but also contributes to our knowledge regarding the possible origins of the surface damage observed on early medieval sculptures made largely from walrus tusks, referred to as 'the Lewis hoard of gaming pieces', that were presumably produced for playing chess. The experiments have shown that the possibility of damage to ivory being caused by fungi is realistic. Scanning electron microscopy revealed penetration of fungal hyphae within cracks in the walrus tusk that showed also widespread tunnelling by fungal hyphae as well as 'fungal footprints' where the surface was etched as a consequence of mycelial colonization. Similar phenomena were observed with boar tusk ivory, while production of metabolites could lead to complete dissolution of the sample. Colonization of ivory and/or exposure to fungal activity lead to extensive secondary biomineral formation, and this was identified as calcium oxalate, mainly as the monohydrate, whewellite.
authors.affiliation: Istituto Centrale per il Restauro e la Conservazione del Patrimonio Archivistico e Librario, Via Milano 76, 00184, Rome, Italy.
authors.forename: Flavia
authors.initials: F
authors.lastname: Pinzari
authors.forename: James
authors.initials: J
authors.lastname: Tate
authors.forename: Marina
authors.initials: M
authors.lastname: Bicchieri
authors.forename: Young Joon
authors.initials: YJ
authors.lastname: Rhee
authors.forename: Geoffrey Michael
authors.initials: GM
authors.lastname: Gadd
mesh_terms: D000818:Animals; D001031:Apatites / Q000378:metabolism*; D001234:Aspergillus niger / Q000378:metabolism* / Q000648:ultrastructure; D001487:Basidiomycota / Q000378:metabolism; D001673:Biodegradation, Environmental; D017400:Brachyspira / Q000378:metabolism* / Q000648:ultrastructure; D002129:Calcium Oxalate / Q000378:metabolism; D025301:Hyphae / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D013552:Swine; D014070:Tooth / Q000378:metabolism* / Q000382:microbiology* / Q000648:ultrastructure; D014856:Walruses
chemical_list: D001031:Apatites; C016189:whewellite; D002129:Calcium Oxalate