title: In vitro 3D full-thickness skin-equivalent tissue model using silk and collagen biomaterials.
abstract: Current approaches to skin equivalents often only include the epidermis and dermis. Here, a full-thickness skin equivalent is described including epidermis, dermis, and hypodermis, that could serve as an in vitro model for studying skin biology or as a platform for consumer product testing. The construct is easy to handle and is maintained for >14 d while expressing physiological morphologies of the epidermis and dermis, seen by keratin 10, collagens I and IV expression. The skin equivalent produces glycerol and leptin, markers of adipose metabolism. This work serves as a foundation for understanding a few necessary factors needed to develop a stable, functional model of full-thickness skin.
authors.affiliation: Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA.
authors.forename: Evangelia
authors.initials: E
authors.lastname: Bellas
authors.forename: Miri
authors.initials: M
authors.lastname: Seiberg
authors.forename: Jonathan
authors.initials: J
authors.lastname: Garlick
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000826:Animal Testing Alternatives / Q000379:methods*; D003094:Collagen / Q000737:chemistry*; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000166:cytology; D020869:Gene Expression Profiling; D006652:Histological Techniques; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000166:cytology; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D047011:Silk / Q000737:chemistry*; D012867:Skin / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology; D046509:Tissue Culture Techniques; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D017931:DNA Primers; D047011:Silk; D003094:Collagen