title: Flavonoids and linderone from Lindera oxyphylla and their bioactivities.
abstract: A new linderone A, namely 2-cinnamoyl-3-hydroxy-4, 5-dimethoxycyclopenta-2, 4-dienone (5), together with three known flavonoids (1-3) and one linderone (4), were isolated from the bark of Lindera oxyphylla. Extensive spectroscopic analysis including 1D and 2D-NMR spectra determined their sturctures. In addition, the antioxidant activity of all the compounds has been determined using 2, 2-diphenyl-1-picrylhydrazyl radical scavenging (DPPH), ferric reducing antioxidant power (FRAP) and ferrous ion chelating (FIC) methods. Compound 3 showed excellent DPPH scavenging activity with IC50% value of 8.5 ± 0.004% (μg/mL) which is comparable with vitamin C. This compound, also showed an absorbance value of 1.00 ± 0.06% through FRAP test when compared with Butyl Hydroxy Aniline (BHA). However, FIC showed low activity for all the isolated compounds (chelating activity less than 50%) in comparison with ethylene diamine tetra acetic acid (EDTA). Anticancer activity for all compounds has also been measured on A375 human melanoma, HT-29 colon adenocarcinoma, MCF-7 human breast adenocarcinoma cells, WRL-68 normal hepatic cells, A549 non-small cell lung cancer cells and PC-3 prostate adenocarcinoma cell line. Compound 1 showed A549=65.03%, PC-3=30.12%, MCF-7=47.67, compound 2 showed PC-3=90.13%, compound 3 showed MCF-7=79.57 and for compound 5 MCF-7 is 96.33.
authors.affiliation: Centre for Natural Products and Drug Discovery, Block D, Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Hosseinzadeh
authors.forename: A Hamid A
authors.initials: AH
authors.lastname: Hadi
authors.forename: Jamaludin
authors.initials: J
authors.lastname: Mohamad
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: Khalilzadeh
authors.forename: Shiau-Chuen
authors.initials: SC
authors.lastname: Cheahd
authors.forename: Mehran
authors.initials: M
authors.lastname: Fadaeinasab
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002614:Chelating Agents / Q000737:chemistry / Q000494:pharmacology; D005419:Flavonoids / Q000737:chemistry* / Q000494:pharmacology; D005609:Free Radicals / Q000737:chemistry; D006801:Humans; D027443:Lindera / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy; D010851:Picrates / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D002614:Chelating Agents; D005419:Flavonoids; D005609:Free Radicals; D010851:Picrates; C584499:linderone A; C004931:1,1-diphenyl-2-picrylhydrazyl