title: The discovery of new cyanobactins from Cyanothece PCC 7425 defines a new signature for processing of patellamides.
abstract: Cyanobactins, including patellamides, are a group of cyanobacterial post-translationally modified ribosomal cyclic peptides. The final product should in theory be predictable from the sequence of the precursor peptide and the associated tailoring enzymes. Understanding the mechanism and recognition requirements of these enzymes will allow their rational engineering. We have identified three new cyanobactins from a Cyanothece PCC 7425 culture subjected to a heat shock. One of these compounds revealed a novel signature signal for ThcA, the subtilisin-like serine protease that is homologous to the patellamide protease PatA. The crystal structure of the latter and modelling studies allow rationalisation of the recognition determinants for both enzymes, consistent with the ribosomal biosynthetic origin of the new compounds.
authors.affiliation: Marine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, UK.
authors.forename: Wael E
authors.initials: WE
authors.lastname: Houssen
authors.forename: Jesko
authors.initials: J
authors.lastname: Koehnke
authors.forename: David
authors.initials: D
authors.lastname: Zollman
authors.forename: Jeremie
authors.initials: J
authors.lastname: Vendome
authors.forename: Andrea
authors.initials: A
authors.lastname: Raab
authors.forename: Margaret C M
authors.initials: MC
authors.lastname: Smith
authors.forename: James H
authors.initials: JH
authors.lastname: Naismith
authors.forename: Marcel
authors.initials: M
authors.lastname: Jaspars
mesh_terms: D000595:Amino Acid Sequence; D046889:Cyanothece / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000737:chemistry / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D057057:Serine Proteases / Q000737:chemistry / Q000378:metabolism
chemical_list: D010456:Peptides, Cyclic; D057057:Serine Proteases