title: The preventive effect of Se-methylselenocysteine on γ-radiation-induced oxidative stress in rat lungs.
abstract: We investigated the preventive effect of Se-methylselenocysteine (MSC) administration on γ-radiation (whole body irradiation, single 10-Gy dose)-induced oxidative damage in rat lungs. Rats were pretreated with MSC (0.75mg/rat/day) for 1 week before γ-irradiation. The MSC pretreatment prevented the irradiation-induced increase in lipid peroxidation and the concomitant decrease in cellular glutathione content. The prevention of irradiation-induced oxidative damage in MSC-pretreated rat lungs appeared to be associated with increased antioxidant capacity, particularly in the glutathione system. The 1-week MSC treatment resulted in an increase in glutathione peroxidase, glutathione reductase, and glucose 6-phosphate dehydrogenase activities, which are involved in glutathione redox cycling. An increase in catalase activity was also observed in the rat lungs. Additionally, a significantly increased level of nuclear factor erythroid 2-related factor 2 (Nrf2) was exhibited in the MSC-treated rat lungs. Heme oxygenase 1, glutathione S-transferase pi, and peroxiredoxin 1, which are known target proteins of Nrf2, were also increased in MSC-treated lungs. These results implicate Nrf2 signaling in the MSC-induced activation of the antioxidant system.
authors.affiliation: Department of Chemistry, University of Incheon, Incheon, Republic of Korea.
authors.forename: Ho-Sang
authors.initials: HS
authors.lastname: Shin
authors.forename: Woo-Jung
authors.initials: WJ
authors.lastname: Yang
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Choi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D005720:Gamma Rays / Q000009:adverse effects*; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D008168:Lung / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology* / Q000528:radiation effects; D008297:Male; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017279:Selenocysteine / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D051267:NF-E2-Related Factor 2; D011837:Radiation-Protective Agents; D017279:Selenocysteine; D005978:Glutathione; C002979:selenomethylselenocysteine