title: Measuring uptake dynamics of multiple identifiable carbon nanotube species via high-speed confocal Raman imaging of live cells.
abstract: Carbon nanotube uptake was measured via high-speed confocal Raman imaging in live cells. Spatial and temporal tracking of two cell-intrinsic and nine nanotube-derived Raman bands was conducted simultaneously in RAW 264.7 macrophages. Movies resolved single (n, m) species, defects, and aggregation states of nanotubes transiently as well as the cell position, denoted by lipid and protein signals. This work portends the real-time molecular imaging of live cells and tissues using Raman spectroscopy, affording multiplexing and complete photostability.
authors.affiliation: Laser Biomedical Research Center, G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.
authors.forename: Jeon Woong
authors.initials: JW
authors.lastname: Kang
authors.forename: Freddy T
authors.initials: FT
authors.lastname: Nguyen
authors.forename: Niyom
authors.initials: N
authors.lastname: Lue
authors.forename: Ramachandra R
authors.initials: RR
authors.lastname: Dasari
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Heller
mesh_terms: D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods; D037742:Nanotubes, Carbon / Q000032:analysis*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D037742:Nanotubes, Carbon