title: Diagnostic assays for Crimean-Congo hemorrhagic fever.
abstract: Crimean-Congo hemorrhagic fever (CCHF) is a highly contagious viral tick-borne disease with case-fatality rates as high as 50%. We describe a collaborative evaluation of the characteristics, performance, and on-site applicability of serologic and molecular assays for diagnosis of CCHF. We evaluated ELISA, immunofluorescence, quantitative reverse transcription PCR, and low-density macroarray assays for detection of CCHF virus using precharacterized archived patient serum samples. Compared with results of local, in-house methods, test sensitivities were 87.8%-93.9% for IgM serology, 80.4%-86.1% for IgG serology, and 79.6%-83.3% for genome detection. Specificity was excellent for all assays; molecular test results were influenced by patient country of origin. Our findings demonstrate that well-characterized, reliable tools are available for CCHF diagnosis and surveillance. The on-site use of such assays by health laboratories would greatly diminish the time, costs, and risks posed by the handling, packaging, and shipping of highly infectious biologic material.
authors.affiliation: Institut Pasteur, Paris, France. jvhomweg@pasteur.fr
authors.forename: Jessica
authors.initials: J
authors.lastname: Vanhomwegen
authors.forename: Maria João
authors.initials: MJ
authors.lastname: Alves
authors.forename: Tatjana Avšič
authors.initials: TA
authors.lastname: Zupanc
authors.forename: Silvia
authors.initials: S
authors.lastname: Bino
authors.forename: Sadegh
authors.initials: S
authors.lastname: Chinikar
authors.forename: Helen
authors.initials: H
authors.lastname: Karlberg
authors.forename: Gülay
authors.initials: G
authors.lastname: Korukluoğlu
authors.forename: Miša
authors.initials: M
authors.lastname: Korva
authors.forename: Masoud
authors.initials: M
authors.lastname: Mardani
authors.forename: Ali
authors.initials: A
authors.lastname: Mirazimi
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Mousavi
authors.forename: Anna
authors.initials: A
authors.lastname: Papa
authors.forename: Ana
authors.initials: A
authors.lastname: Saksida
authors.forename: Batool
authors.initials: B
authors.lastname: Sharifi-Mood
authors.forename: Persofoni
authors.initials: P
authors.lastname: Sidira
authors.forename: Katerina
authors.initials: K
authors.lastname: Tsergouli
authors.forename: Roman
authors.initials: R
authors.lastname: Wölfel
authors.forename: Hervé
authors.initials: H
authors.lastname: Zeller
authors.forename: Philippe
authors.initials: P
authors.lastname: Dubois
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006479:Hemorrhagic Fever, Crimean / Q000175:diagnosis*; D006801:Humans; D046228:Microarray Analysis; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012698:Serologic Tests
chemical_list: D011933:Reagent Kits, Diagnostic