title: Pilot study of changes in salivary metabolic profiles induced by template therapy.
abstract: BACKGROUND
  Occlusal raising method (so-called 'Template therapy') has been reported to alleviate various diseases and symptoms, but the underlying mechanism is not clear. We searched the low-molecular weight metabolite(s) in the saliva, the concentration of which is significantly changed by the template therapy.
  
  
  METHODS
  One female patient with headache underwent the template therapy for 12 days, and her total saliva was subjected to non-targeted analysis using capillary electrophoresis time-of-flight mass spectrometry (CE-TOF-MS).
  
  
  RESULTS
  One hundred and thirteen substances were identified in the saliva. Glycine was the most abundant amino acid in the saliva, followed by alanine, serine and proline. After the start of the template therapy, her headache was alleviated, accompanied by a significant (p=0.042) increase of salivary concentration of glycine, as compared with total amino acids whereas that of other amino acids was not significantly changed. In the metabolomics profile, salivary concentration of large number of metabolites as compared with total metabolite concentration decreased, including N-acetylneuraminate (p=0.025) and p-hydroxyphenylacetate (p=0.039).
  
  
  CONCLUSIONS
  This pilot study demonstrated, to our knowledge for the first time, that only glycine exhibited unique changes among total metabolites, suggesting its significant role in template therapy.
authors.affiliation: Division of Oral Diagnosis, Department of Diagnostic and Therapeutic Sciences, Meikai University School of Dentistry, Sakado, Saitama, Japan. stanaka@dent.meikai.ac.jp
authors.forename: Shoji
authors.initials: S
authors.lastname: Tanaka
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Taga
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Maehara
authors.forename: Azusa
authors.initials: A
authors.lastname: Kaneshima
authors.forename: Mamoru
authors.initials: M
authors.lastname: Machino
authors.forename: Hiromi
authors.initials: H
authors.lastname: Onuma
authors.forename: Miku
authors.initials: M
authors.lastname: Kaneko
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sakagami
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sugimoto
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Soga
authors.forename: Masaru
authors.initials: M
authors.lastname: Tomita
mesh_terms: D000328:Adult; D005260:Female; D005998:Glycine / Q000032:analysis; D006261:Headache / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D055432:Metabolomics; D008970:Molecular Weight; D010865:Pilot Projects; D025341:Principal Component Analysis; D012463:Saliva / Q000378:metabolism*; D012469:Salivary Glands / Q000378:metabolism*
chemical_list: D005998:Glycine