title: Differential expression of microRNAs in avian leukosis virus subgroup J-induced tumors.
abstract: Avian leukosis virus subgroup J (ALV-J) has become pandemic and induced serious clinical outbreaks in chickens in China. In particular, ALV-J induced various clinical tumors in infected chickens, which caused enormous economic losses to poultry. In this study, an infectious clone from an epidemic ALV-J Chinese isolate designated HLJ09SH01 was constructed and rescued. The rescued virus (named rHLJ09SH01) was inoculated into specific-pathogen-free (SPF) layer chickens, and infected chickens were observed for 238 days to explore the oncogenicity of rHLJ09SH01. As a result, 57.9% of rHLJ09SH01-infected chickens produced tumors. Accumulating evidence shows that microRNAs (miRNAs) have a close relationship with tumorigenesis. To gain more insight into the tumorigenesis of ALV-J, a miRNA microarray was performed as part of an investigation of changes in host miRNA expression in a liver tumor from ALV-J infected chickens. The results showed that four miRNAs were significantly differentially expressed; these data were verified using real-time PCR. Bioinformatics analysis showed the differentially expressed miRNAs to be involved in some tumorigenesis-related signaling pathways, such as the MAPK signaling pathway and the Wnt signaling pathway, which may represent a possible signaling pathway that was involved in the ALV-J-induced tumorigenesis.
authors.affiliation: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Yulong
authors.initials: Y
authors.lastname: Gao
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Ji
authors.forename: Xiaole
authors.initials: X
authors.lastname: Qi
authors.forename: Liting
authors.initials: L
authors.lastname: Qin
authors.forename: Honglei
authors.initials: H
authors.lastname: Gao
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D001353:Avian Leukosis / Q000473:pathology / Q000821:virology*; D001354:Avian Leukosis Virus / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D063646:Carcinogenesis; D002642:Chick Embryo; D002645:Chickens; D002681:China; D016679:Genome, Viral; D008460:Meat / Q000821:virology; D053858:Metabolic Networks and Pathways / Q000235:genetics; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D011201:Poultry Diseases / Q000821:virology*; D011533:Proviruses / Q000235:genetics; D013047:Specific Pathogen-Free Organisms
chemical_list: D035683:MicroRNAs