title: Study of molecule variations in renal tumor based on confocal micro-Raman spectroscopy.
abstract: Confocal micro-Raman spectroscopy--a valuable analytical tool in biological and medical field of research-allows probing molecular vibrations of samples without external labels or extensive preparation. We employ confocal micro-Raman spectroscopy to characterize renal tumors and normal tissue. Results show that Raman peaks of the renal tumor at 788 and 1087  cm-1, which belong to (ν)(s)PO(2)- and (ν)(as)PO(2)- stretching, respectively, have an obvious increase. At the same time, the ratio of I855/I831 in renal tumor tissue is 1.39 ± 0.08, while that in normal renal tissue is 2.44 ± 0.05 (p < 0.01). This means that more tyrosine conformation transform from "buried" to "exposed" in the presence of cancer. Principal component analysis is used to classify the Raman spectra of renal tumor tissue and normal tissue.
authors.affiliation: South China Normal University, College of Biophotonics, MOE Key Laboratory of Laser Life Science, Guangzhou 510631, Guangdong, China. ann@scnu.edu.cn
authors.forename: Zhengfei
authors.initials: Z
authors.lastname: Zhuang
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Zhouyi
authors.initials: Z
authors.lastname: Guo
authors.forename: Meifang
authors.initials: M
authors.lastname: Zhu
authors.forename: Ke
authors.initials: K
authors.lastname: Xiong
authors.forename: Sijin
authors.initials: S
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000737:chemistry* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D010100:Oxygen / Q000737:chemistry; D025341:Principal Component Analysis; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014443:Tyrosine / Q000737:chemistry
chemical_list: D014443:Tyrosine; D010100:Oxygen