title: A new pentaplex-nested PCR to detect five pathogenic bacteria in free living amoebae.
abstract: Changes in water use and anthropogenic activity have major impacts on the quality of natural aquatic ecosystems, water distribution and wastewater plants. One of the main problems is the presence of some pathogenic microorganisms that are resistant to disinfection procedures when they are hosted by free living amoeba and that in many cases are hardly detectable by culture-based procedures. In this work we report a sensitive, low-cost procedure consisting of a pentaplex-nested PCR that allows simultaneous detection of Legionella pneumophila, Mycobacterium spp., Pseudomonas spp., Vibrio cholerae and the microcystin-producing cyanobacteria Microcystis aeruginosa. The method has been used to detect the presence of these pathogenic bacteria in water and inside free living amoeba. Its validation in 72 samples obtained from different water sources from Aragon (Spain) evidences that Mycobacterium and Pseudomonas spp are prevailing as amoeba-resistant bacteria.
authors.affiliation: Department of Biochemistry and Molecular and Cell Biology, Faculty of Sciences, University of Zaragoza, Pedro Cerbuna 12, 50009 Zaragoza, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Calvo
authors.forename: I
authors.initials: I
authors.lastname: Gregorio
authors.forename: A
authors.initials: A
authors.lastname: García
authors.forename: M T
authors.initials: MT
authors.lastname: Fernández
authors.forename: P
authors.initials: P
authors.lastname: Goñi
authors.forename: A
authors.initials: A
authors.lastname: Clavel
authors.forename: M L
authors.initials: ML
authors.lastname: Peleato
authors.forename: M F
authors.initials: MF
authors.lastname: Fillat
mesh_terms: D000656:Amoeba / Q000254:growth & development / Q000382:microbiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D016952:Legionella pneumophila / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D057230:Limit of Detection; D050296:Microbial Viability; D046931:Microcystis / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D058889:Molecular Typing / Q000379:methods; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D015203:Reproducibility of Results; D012722:Sewage / Q000382:microbiology / Q000469:parasitology; D013030:Spain; D014734:Vibrio cholerae / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D014871:Water Microbiology; D014876:Water Pollution / Q000032:analysis; D062066:Water Resources / Q000032:analysis; D014881:Water Supply / Q000032:analysis
chemical_list: D001426:Bacterial Proteins; D012722:Sewage