title: Vegetables and PUFA-rich plant oil reduce DNA strand breaks in individuals with type 2 diabetes.
abstract: METHODS
  Type 2 diabetes is a multifactorial disease associated with increased oxidative stress, which may lead to increased DNA damage. The aim of this study was to investigate the effect of a healthy diet on DNA oxidation in diabetics and nondiabetics.
  
  
  RESULTS
  Seventy-six diabetic and 21 nondiabetic individuals participated in this study. All subjects received information about the benefits of a healthy diet, while subjects randomly assigned to the intervention group received additionally 300 g of vegetables and 25 mL PUFA-rich plant oil per day. DNA damage in mononuclear cells (Comet Assay), urinary excretion of 8-oxo-7-hydro-2'-deoxyguanosine (8-oxodG) and 8-oxo-7,8-dihydroguanosine (8-oxoGuo) and glycated hemoglobin (HbA1c) were measured at baseline, after 4, 8 (end of intervention), and 16 weeks. The intervention with vegetables and PUFA-rich oil led to a significant increase in plasma antioxidant concentrations. Diabetic individuals of the intervention group showed a significant reduction in HbA1c and DNA strand breaks. Levels of HbA1c were also improved in diabetics of the information group, but oxidative damage to DNA was not altered. Urinary 8-oxodG and 8-oxoGuo excretion remained unchanged in both groups.
  
  
  CONCLUSIONS
  This study provides evidence that a healthy diet rich in antioxidants reduces levels of DNA strand breaks in diabetic individuals.
authors.affiliation: Department of Nutritional Sciences, Emerging Field Oxidative Stress and DNA Stability, University of Vienna, Vienna, Austria. elisabeth.muellner@univie.ac.at
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Müllner
authors.forename: Helmut
authors.initials: H
authors.lastname: Brath
authors.forename: Simone
authors.initials: S
authors.lastname: Pleifer
authors.forename: Christiane
authors.initials: C
authors.lastname: Schiermayr
authors.forename: Andreas
authors.initials: A
authors.lastname: Baierl
authors.forename: Marlies
authors.initials: M
authors.lastname: Wallner
authors.forename: Theresia
authors.initials: T
authors.lastname: Fastian
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Millner
authors.forename: Kristina
authors.initials: K
authors.lastname: Paller
authors.forename: Trine
authors.initials: T
authors.lastname: Henriksen
authors.forename: Henrik Enghusen
authors.initials: HE
authors.lastname: Poulsen
authors.forename: Ernst
authors.initials: E
authors.lastname: Forster
authors.forename: Karl-Heinz
authors.initials: KH
authors.lastname: Wagner
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D000975:Antioxidants / Q000032:analysis; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D003924:Diabetes Mellitus, Type 2 / Q000178:diet therapy*; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage*; D005260:Female; D006442:Glycated Hemoglobin / Q000652:urine; D038321:Glycemic Index; D006151:Guanosine / Q000031:analogs & derivatives / Q000652:urine; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D010349:Patient Compliance; D010938:Plant Oils / Q000008:administration & dosage; D014675:Vegetables
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000975:Antioxidants; D003849:Deoxyguanosine; D005231:Fatty Acids, Unsaturated; D006442:Glycated Hemoglobin; D006151:Guanosine; D010938:Plant Oils; C046215:8-hydroxyguanosine