title: Fibrous nanostructures from the self-assembly of designed repeat protein modules.
abstract: Single-protein-chain superhelical filaments are obtained from monomeric repeat proteins by controlling the chemistry and solvent exposure at their terminal interfaces. The assembly was achieved in aqueous solution, at neutral pH value, and at room temperature. The building block was a recombinantly engineered designed tetratricopeptide repeat protein. Directed head-to-tail self-assembly was driven by genetically encoded orthogonal native chemical ligation.
authors.affiliation: School of Biological and Chemical Sciences, Queen Mary University of London, UK.
authors.forename: Jonathan J
authors.initials: JJ
authors.lastname: Phillips
authors.forename: Charlotte
authors.initials: C
authors.lastname: Millership
authors.forename: Ewan R G
authors.initials: ER
authors.lastname: Main
mesh_terms: D006801:Humans; D006863:Hydrogen-Ion Concentration; D049329:Nanostructures / Q000737:chemistry*; D015202:Protein Engineering; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D013696:Temperature; D044767:Ubiquitin-Protein Ligases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D011506:Proteins; D011994:Recombinant Proteins; C108062:TTC3 protein, human; D044767:Ubiquitin-Protein Ligases