title: Schistosoma japonicum soluble egg antigens attenuate IFN-γ-induced MHC class II expression in RAW 264.7 macrophages.
abstract: Innate immune response plays the key role in initiating and guiding the immune response. Elucidating the innate immune related molecular events involved in the interaction between the parasite and the host will aid in the development of an effective vaccine and anti-schistosome pharmaceuticals. In this study, we examined the regulatory effect of Schistosoma japonicum soluble egg antigen (SEA) on MHC class II expression in macrophage cell line RAW 264.7. We demonstrated that SEA possesses the ability to down-regulate IFN-γ-induced MHC class II expression in RAW 264.7 cells. The production of IL-10 and IL-6 in RAW 264.7 cells, induced by SEA, was responsible for mediating the down-regulation of MHC class II. Our findings suggest that in RAW 264.7 cells (1) IFN-γ provides a condition for lower concentrations of SEA to attenuate MHC class II expression; (2) SEA attenuated IFN-γ-induced MHC class II expression and the IL-10 and IL-6 production is mediated at least partly by the interaction of SEA with TLR4; and (3) SEA attenuated IFN-γ-induced MHC class II expression at the transcriptional level.
authors.affiliation: Department of Pathogen Biology and Immunology, Nanjing Medical University, Nanjing, Jiangsu, China. guixiatang@163.com
authors.forename: Gui-Xia
authors.initials: GX
authors.lastname: Tang
authors.forename: He-Jun
authors.initials: HJ
authors.lastname: Zhou
authors.forename: Jin-Wei
authors.initials: JW
authors.lastname: Xu
authors.forename: Jin-Mei
authors.initials: JM
authors.lastname: Xu
authors.forename: Min-Jun
authors.initials: MJ
authors.lastname: Ji
authors.forename: Hai-Wei
authors.initials: HW
authors.lastname: Wu
authors.forename: Guan-Ling
authors.initials: GL
authors.lastname: Wu
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D002478:Cells, Cultured; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D007371:Interferon-gamma / Q000494:pharmacology*; D016753:Interleukin-10 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010063:Ovum / Q000187:drug effects / Q000276:immunology*; D011817:Rabbits; D012549:Schistosoma japonicum / Q000276:immunology*; D051197:Toll-Like Receptor 4 / Q000378:metabolism
chemical_list: D000947:Antigens, Helminth; D000949:Histocompatibility Antigens Class II; D015850:Interleukin-6; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D016753:Interleukin-10; D007371:Interferon-gamma