title: Effect of leukoreduction treatment on vascular endothelial growth factor concentration in stored canine blood transfusion products.
abstract: OBJECTIVE
  To evaluate vascular endothelial growth factor (VEGF) concentrations in canine blood products treated with or without a leukoreduction filter.
  
  
  METHODS
  10 canine blood donors.
  
  
  METHODS
  Dogs underwent blood collection. Five of 10 units were leukoreduced prior to separation into packed RBCs and fresh frozen plasma (FFP). Concentrations of VEGF were measured by ELISA in plasma supernatants from aliquots of packed RBCs obtained immediately after separation and on days 7, 14, and 21 of storage. Fresh frozen plasma samples of 2 filtered and 2 nonfiltered units were examined after storage.
  
  
  RESULTS
  RBC counts in whole blood before and after leukoreduction did not differ significantly, but WBCs and platelets were removed effectively. The VEGF concentration was lower than the detection limit (9 pg/mL) in 9 of 10 plasma samples and in all packed RBC and FFP units immediately after separation. The median VEGF concentrations in 5 nonfiltered packed RBC units were 37, 164, and 110 pg/mL on days 7, 14, and 21 of storage, respectively. In 5 filtered packed RBC and all FFP units, VEGF concentrations remained lower than the detection limit.
  
  
  CONCLUSIONS
  Leukoreduction filters were effective in preventing the release of VEGF during storage of canine RBC products.
authors.affiliation: Clinic of Small Animals, Faculty of Veterinary Medicine, Freie Universität Berlin, D-14163 Berlin, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Graf
authors.forename: Jens
authors.initials: J
authors.lastname: Raila
authors.forename: Florian J
authors.initials: FJ
authors.lastname: Schweigert
authors.forename: Barbara
authors.initials: B
authors.lastname: Kohn
mesh_terms: D000818:Animals; D001793:Blood Preservation / Q000379:methods* / Q000662:veterinary; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004912:Erythrocytes / Q000166:cytology*; D005260:Female; D005374:Filtration / Q000379:methods / Q000662:veterinary; D047589:Leukocyte Reduction Procedures / Q000379:methods* / Q000662:veterinary; D007962:Leukocytes / Q000166:cytology*; D008297:Male; D010949:Plasma / Q000166:cytology*; D011897:Random Allocation; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis / Q000097:blood
chemical_list: D042461:Vascular Endothelial Growth Factor A