title: Characterization of the metabolic pathway involved in assimilation of acenaphthene in Acinetobacter sp. strain AGAT-W.
abstract: Utilization of an enrichment technique led to isolation of a bacterium from municipal waste-contaminated soil in which acenaphthene was used as the sole source of carbon and energy. The isolate was identified as Acinetobacter sp. strain AGAT-W based on morphological, nutritional and biochemical characteristics and 16S rRNA sequence analysis. Characterization of metabolites by HPLC and GC-MS suggested hydroxylation of acenaphthene to 1-acenaphthenol, which was subsequently transformed to catechol via acenaphthenequinone, naphthalene-1,8-dicarboxylic acid, 1-naphthoic acid and salicylic acid before entering into the tricarboxylic acid cycle. Detection of key enzymes, viz., 1-acenaphthenol dehydrogenase, salicylaldehyde dehydrogenase and catechol 1,2-dioxygenase, in the cell-free extract of Acinetobacter sp. further supported the proposed degradation pathway. This study proposes a metabolic pathway involved in acenaphthene assimilation in strain AGAT-W.
authors.affiliation: Department of Microbiology, Bose Institute, P-1/12 C.I.T. Scheme VII M, Kolkata 700054, India. debajyoti_mcb@yahoo.com
authors.forename: Debajyoti
authors.initials: D
authors.lastname: Ghosal
authors.forename: Arindam
authors.initials: A
authors.lastname: Dutta
authors.forename: Joydeep
authors.initials: J
authors.lastname: Chakraborty
authors.forename: Soumik
authors.initials: S
authors.lastname: Basu
authors.forename: Tapan K
authors.initials: TK
authors.lastname: Dutta
mesh_terms: D000073:Acenaphthenes / Q000378:metabolism*; D000150:Acinetobacter / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D001711:Biotransformation; D002244:Carbon / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004734:Energy Metabolism; D008401:Gas Chromatography-Mass Spectrometry; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D000073:Acenaphthenes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002244:Carbon; C042552:acenaphthene