title: A gene-networked gel matrix-supported lipid bilayer as a synthetic nucleus system.
abstract: A spheroidal transgene-networked gel matrix was designed as a synthetic nucleus system. It was spheroidically manufactured using both advanced lithography and DNA nanotechnology. Stable Aqueorea coerulescens green fluorescent protein (AcGFP)-encoding gene cross-networks have been optimized in various parameters: the number of gene-networked gel (G-net-gel) spheroids, the concentration of a AcGFP plasmid in the scaffold, and the molar ratio between the X-DNA building blocks and the gene. It was then assessed that 800 units of the gene networked gel matrix at a 4000:1 molar ratio of X-DNA blocks and AcGFP gene components accomplished 20-fold enhanced in vitro protein expression efficiency for 36 h. Furthermore, once with lipid capping, it reproduced the natural nucleus system, demonstrating the 2-fold increased levels of messenger RNAs (mRNAs) relative to solution phase vectors.
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, Suwon, Gyeonggi-do, 440-760, South Korea.
authors.forename: Sun Ju
authors.initials: SJ
authors.lastname: Bae
authors.forename: Woo Chul
authors.initials: WC
authors.lastname: Song
authors.forename: Sung Hwan
authors.initials: SH
authors.lastname: Jung
authors.forename: Seung-Woo
authors.initials: SW
authors.lastname: Cho
authors.forename: Dong-Ik
authors.initials: DI
authors.lastname: Kim
authors.forename: Soong Ho
authors.initials: SH
authors.lastname: Um
mesh_terms: D002467:Cell Nucleus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D045566:DNA, Cruciform / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D005782:Gels / Q000737:chemistry* / Q000378:metabolism; D053263:Gene Regulatory Networks; D049452:Green Fluorescent Proteins; D057927:Hydrophobic and Hydrophilic Interactions; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D008954:Models, Biological; D009690:Nucleic Acid Conformation; D010957:Plasmids; D014176:Protein Biosynthesis
chemical_list: D045566:DNA, Cruciform; D004277:DNA, Single-Stranded; D004129:Dimethylpolysiloxanes; D005782:Gels; D008051:Lipid Bilayers; D049452:Green Fluorescent Proteins