title: ELISA and immuno-polymerase chain reaction assays for the sensitive detection of melioidosis.
abstract: Melioidosis is caused by the Gram-negative bacterium Burkholderia pseudomallei. The gold standard for diagnosis is culture, which requires at least 3-4 days to obtain a result, hindering successful treatment of acute disease. An indirect haemagglutination assay (IHA) is often used but lacks sensitivity. Approximately half of patients later confirmed culture positive are not detected by IHA at presentation and a subset of patients persistently continue to be IHA negative. More rapid and reliable serologic testing for melioidosis is essential and will improve diagnosis and patient outcome. We have developed an ELISA and a quantitative immuno-polymerase chain reaction assay capable of detecting melioidosis-specific antibodies and demonstrate their validity with IHA-negative sera from patients with melioidosis. These new sensitive assays are based upon a secreted antigenic fraction from B. pseudomallei and will be ideal for the diagnosis of melioidosis in patients in nonendemic regions returning from endemic tropical areas and for seroepidemiologic surveys.
authors.affiliation: School of Pharmacy and Molecular Sciences, James Cook University, Douglas, QLD 4811, Australia.
authors.forename: Alanna
authors.initials: A
authors.lastname: Cooper
authors.forename: Natasha L
authors.initials: NL
authors.lastname: Williams
authors.forename: Jodie L
authors.initials: JL
authors.lastname: Morris
authors.forename: Robert E
authors.initials: RE
authors.lastname: Norton
authors.forename: Natkunam
authors.initials: N
authors.lastname: Ketheesan
authors.forename: Patrick M
authors.initials: PM
authors.lastname: Schaeffer
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D016957:Burkholderia pseudomallei / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D008554:Melioidosis / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial