title: In vitro phosphorylation of the N-terminal half of hordeivirus movement protein.
abstract: The N-terminal half of TGB1 movement protein of poa semilatent hordeivirus, which forms a ribonucleoprotein complex involved in movement of the viral genome in the plant, and its two domains, NTD and ID, are phosphorylated in vitro by a fraction enriched in cell walls from Nicotiana benthamiana. Using a set of protein kinase inhibitors with different specificities, it was found that enzymes possessing activities of casein kinase 1, protein kinase A, and protein kinase C are involved in phosphorylation. Commercial preparations of protein kinases A and C are able to phosphorylate in vitro recombinant proteins corresponding to the N-terminal half of the protein and its domains NTD and ID. Phosphorylation of the NTD has no effect on the efficiency and character of its binding to RNA. However, phosphorylation of the ID leads to a decrease in its RNA-binding activity and in the ability for homological protein-protein interactions.
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Makarov
authors.forename: A Y
authors.initials: AY
authors.lastname: Iconnikova
authors.forename: M A
authors.initials: MA
authors.lastname: Guseinov
authors.forename: V K
authors.initials: VK
authors.lastname: Vishnichenko
authors.forename: N O
authors.initials: NO
authors.lastname: Kalinina
mesh_terms: D047389:Casein Kinase I / Q000037:antagonists & inhibitors / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D053830:Plant Viral Movement Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D010942:Plant Viruses; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D014026:Nicotiana / Q000166:cytology / Q000821:virology
chemical_list: D053830:Plant Viral Movement Proteins; D047428:Protein Kinase Inhibitors; D047389:Casein Kinase I; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C