title: Assessment of ADVIA Centaur analyzer for the measurement of 25-OH vitamin D.
abstract: BACKGROUND
  The aim of the present study was to evaluate some analytical performances of the ADVIA Centaur analyzer for the quantitative measurement of 25-OH Vitamin D [25(OH)D] in serum.
  
  
  METHODS
  Serum concentrations of 25(OH)D were determined by a new automated chemiluminescence immunoassay method introduced by Siemens and adapted to an ADVIA Centaur analyzer, and compared with HPLC and a commercial chemiluminescence immunoassay (Liaison DiaSorin).
  
  
  RESULTS
  The assay displayed a low intra-day (CV < 7.0%) and a low inter-day imprecision (CV < 9.0%). The ADVIA Centaur demonstrated a stronger Spearman's correlation (r = 0.921), better agreement (bias = -0.3 ng/mL), and better concordance correlation coefficient of Lin (P(c) = 0.88) and better kappa index (k = 0.92) with HPLC, than the Liaison DiaSorin assay (r = 0.907, bias = 5.9 ng/mL, p(c) = 0.84, k = 0.60) with HPLC. On the other hand, a significant inverse relationship was observed between parathyroid hormone (PTH) and 25(OH)D concentrations.
  
  
  CONCLUSIONS
  The ADVIA Centaur method is reliable for routine 25(OH)D determination in clinical laboratories
authors.affiliation: Servicio de Análisis Clínicos, Area Sanitaria de Ferrol, Avenida da residencia s/n, 15405 Ferrol, A Coruña, Spain. fernandojesus.hermida.ameijeiras@sergas.es
authors.forename: Fernando Jesús
authors.initials: FJ
authors.lastname: Hermida
authors.forename: María
authors.initials: M
authors.lastname: Fernández
authors.forename: Belén
authors.initials: B
authors.lastname: Laborda
authors.forename: Aida
authors.initials: A
authors.lastname: Pérez
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Lorenzo
authors.forename: Concepción
authors.initials: C
authors.lastname: Magadán
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001322:Autoanalysis; D002648:Child; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods*; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D012044:Regression Analysis; D015203:Reproducibility of Results; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000097:blood; D055815:Young Adult
chemical_list: D007202:Indicators and Reagents; D014807:Vitamin D; C104450:25-hydroxyvitamin D