title: Aeromonas australiensis sp. nov., isolated from irrigation water.
abstract: A Gram-negative, facultatively anaerobic bacillus, designated strain 266(T), was isolated from an irrigation water system in the south-west of Western Australia. Analysis of the 16S rRNA gene sequence confirmed that strain 266(T) belonged to the genus Aeromonas, with the nearest species being Aeromonas fluvialis (99.6% similarity to the type strain, with 6 nucleotide differences) followed by Aeromonas veronii and Aeromonas allosaccharophila (both 99.5%). Analysis of gyrB and rpoD sequences suggested that strain 266(T) formed a phylogenetic line independent of other species in the genus. This was confirmed using the concatenated sequences of six housekeeping genes (gyrB, rpoD, recA, dnaJ, gyrA and dnaX) that also indicated that A. veronii and A. allosaccharophila were the nearest relatives. DNA-DNA reassociation experiments and phenotypic analysis further supported the conclusion that strain 266(T) represents a novel species, for which the name Aeromonas australiensis sp. nov. is proposed, with type strain 266(T) (=CECT 8023(T) =LMG 26707(T)). [corrected].
authors.affiliation: Division of Microbiology and Infectious Diseases, PathWest Laboratory Medicine, Nedlands, WA, Australia.|School of Pathology and Laboratory Medicine, the University of Western Australia, Crawley, WA, Australia.
authors.forename: Max
authors.initials: M
authors.lastname: Aravena-Román
authors.affiliation: Unitat de Microbiologia, Department de Ciènces Mèdiques Básiques, Facultat de Medicina i Ciènces de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain.
authors.forename: Roxana
authors.initials: R
authors.lastname: Beaz-Hidalgo
authors.affiliation: Division of Microbiology and Infectious Diseases, PathWest Laboratory Medicine, Nedlands, WA, Australia.|School of Pathology and Laboratory Medicine, the University of Western Australia, Crawley, WA, Australia.
authors.forename: Timothy J J
authors.initials: TJJ
authors.lastname: Inglis
authors.affiliation: Division of Microbiology and Infectious Diseases, PathWest Laboratory Medicine, Nedlands, WA, Australia.|School of Pathology and Laboratory Medicine, the University of Western Australia, Crawley, WA, Australia.
authors.forename: Thomas V
authors.initials: TV
authors.lastname: Riley
authors.affiliation: Departamento de Producción Vegetal y Microbiología, EPSO, Universidad Miguel Hernández, Orihuela 03312 (Alicante), Spain.
authors.forename: Antonio J
authors.initials: AJ
authors.lastname: Martínez-Murcia
authors.affiliation: School of Pathology and Laboratory Medicine, the University of Western Australia, Crawley, WA, Australia.
authors.forename: Barbara J
authors.initials: BJ
authors.lastname: Chang
authors.affiliation: Unitat de Microbiologia, Department de Ciènces Mèdiques Básiques, Facultat de Medicina i Ciènces de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain.
authors.forename: Maria Jose
authors.initials: MJ
authors.lastname: Figueras
mesh_terms: D000333:Aeromonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D057914:Agricultural Irrigation; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology; D014904:Western Australia
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S