title: Short read sequencing in studies of natural variation and adaptation.
abstract: Today's high throughput sequencing approaches, coupled with equally revolutionary advances in bioinformatics, allow us to describe and analyze genomes in unprecedented detail. Short Read Sequencing (SRS) approaches have been especially instrumental in bringing genomic analysis to a wide range of questions and species in plant biology. We can now connect genotypes and phenotypes with greater efficiency, and investigate the molecular basis of natural variation and adaptation in a genomic framework. New and creative applications of SRS and other genomic approaches are not only reshaping how we study natural variation, but also our overall understanding of gene and genome evolution. Here we discuss examples of the application of SRS technologies to the characterization of genetic diversity, genome evolution and adaptation in plants.
authors.affiliation: Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA.
authors.forename: Ben
authors.initials: B
authors.lastname: Hunter
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Wright
authors.forename: Kirsten
authors.initials: K
authors.lastname: Bomblies
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D019295:Computational Biology; D019143:Evolution, Molecular; D014644:Genetic Variation; D018745:Genome, Plant / Q000235:genetics*; D023281:Genomics; D005838:Genotype; D059014:High-Throughput Nucleotide Sequencing; D018521:Plant Physiological Phenomena; D010944:Plants / Q000235:genetics; D017422:Sequence Analysis, DNA