title: Influence of the core circadian gene "Clock" on obesity and leptin resistance in mice.
abstract: Alterations in metabolism could be due to cell-autonomous effects associated with altered expression of Clock in central nervous system feeding centers and/or peripheral tissues involved in metabolism. Clock mutant mice are hyperphagic and obese, which indicates that Clock is related to obesity. In the present study, we used intracerebroventricular injection of recombinant adenoviral vector harboring Clock genes to explore the role of Clock on diet induced obesity and the mechanisms involved in leptin resistance and leptin signaling in mice. The results demonstrated that expression of Clock in the arcuate nucleus of diet induced obesity mice was down-regulated. The recombinant adenoviral vector harboring Clock genes could reduce obesity indexes of diet induced obesity mice including body weight, BMI and total fat mass, attenuate hyperleptinemia, increase leptin sensitivity and decrease accumulated suppressor of cytokine signaling-3 in the arcuate nucleus. These results indicate that Clock plays an important role on obesity, which may be involved in leptin resistance and regulation of suppressor of cytokine signaling-3 in arcuate nucleus.
authors.affiliation: The Medical Center of Dujiangyan, Dujiangyan, Chengdu, Sichuan, PR China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Xie
authors.forename: Shuhong
authors.initials: S
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zou
authors.forename: Shuting
authors.initials: S
authors.lastname: Cheng
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhou
authors.initials: Z
authors.lastname: Jiang
authors.forename: Jing
authors.initials: J
authors.lastname: Xiao
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Wang
authors.forename: Yanyou
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001823:Body Composition / Q000502:physiology; D015992:Body Mass Index; D001835:Body Weight / Q000235:genetics / Q000502:physiology; D002552:Cerebral Ventricles / Q000502:physiology; D056925:Circadian Rhythm Signaling Peptides and Proteins / Q000235:genetics*; D000229:Dependovirus / Q000235:genetics; D005822:Genetic Vectors; D007276:Injections, Intraventricular; D020738:Leptin / Q000235:genetics*; D050356:Lipid Metabolism / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D054411:Receptors, Leptin; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics
chemical_list: D056925:Circadian Rhythm Signaling Peptides and Proteins; D020738:Leptin; D054411:Receptors, Leptin; C491411:Socs3 protein, mouse; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins