title: Relationship between DNA damage in sperm after ex vivo exposure and abnormal embryo development in the progeny of the three-spined stickleback.
abstract: Many xenobiotics released in the aquatic environment exhibit a genotoxic potential toward organisms. Long term exposure to such compounds is expected to lead to multigenerational reproductive defects, further influencing the recruitment rate and hence, the population dynamics. Paternal exposure to genotoxicants was previously shown to increase abnormal development in the progeny of mammalian or aquatic species. The aim of this study was to evaluate the relationship between DNA damage in sperm of the fish three-spined stickleback and progeny developmental defects. Spermatozoa were exposed ex vivo to an alkylating agent (methyl methanesulfonate) before in vitro fertilization and DNA damage was assessed by the alkaline comet assay. A significant relationship between abnormal development and sperm DNA damage was underlined. This study illustrates the interest to use germ cell DNA damage after ex vivo exposure to evaluate the impact of genotoxic compounds on progeny fitness in aquatic organisms.
authors.affiliation: Université de Lyon, UMR 5023 LEHNA, F-69100, Villeurbanne, France.
authors.forename: R
authors.initials: R
authors.lastname: Santos
authors.forename: M
authors.initials: M
authors.lastname: Palos-Ladeiro
authors.forename: A
authors.initials: A
authors.lastname: Besnard
authors.forename: J M
authors.initials: JM
authors.lastname: Porcher
authors.forename: S
authors.initials: S
authors.lastname: Bony
authors.forename: W
authors.initials: W
authors.lastname: Sanchez
authors.forename: A
authors.initials: A
authors.lastname: Devaux
mesh_terms: D000477:Alkylating Agents / Q000633:toxicity*; D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000633:toxicity; D020552:Comet Assay; D004249:DNA Damage; D004625:Embryo, Nonmammalian / Q000002:abnormalities / Q000187:drug effects; D047108:Embryonic Development / Q000187:drug effects*; D005307:Fertilization in Vitro / Q000187:drug effects; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D008297:Male; D008741:Methyl Methanesulfonate / Q000633:toxicity; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens / Q000633:toxicity*; D009994:Osmolar Concentration; D018812:Paternal Exposure / Q000009:adverse effects*; D011897:Random Allocation; D023701:Smegmamorpha / Q000002:abnormalities / Q000196:embryology* / Q000254:growth & development; D013094:Spermatozoa / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D000477:Alkylating Agents; D018906:Antineoplastic Agents, Alkylating; D009153:Mutagens; D014874:Water Pollutants, Chemical; D008741:Methyl Methanesulfonate