title: Fabrication of 3-dimensional cellular constructs via microstereolithography using a simple, three-component, poly(ethylene glycol) acrylate-based system.
abstract: A novel method for the production of inhibitor- and solvent-free resins suitable for three-dimensional (3D) microstereolithography is reported. Using an exemplar poly(ethylene glycol)-based resin, the control of features in the X, Y, and Z planes is demonstrated such that complex structures can be manufactured. Human mesenchymal stem cells cultured on the manufactured scaffolds remained viable during the 7 day assessment period, with proliferation rates comparable to those observed on tissue culture polystyrene. These data suggest that this novel, yet simple, method is suitable for the production of 3D scaffolds for tissue engineering and regenerative medicine applications.
authors.affiliation: Department of Chemistry, University of Warwick, Coventry, CV4 7AL, United Kingdom.
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Leigh
authors.forename: Hamish T J
authors.initials: HT
authors.lastname: Gilbert
authors.forename: Ian A
authors.initials: IA
authors.lastname: Barker
authors.forename: Jan M
authors.initials: JM
authors.lastname: Becker
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Richardson
authors.forename: Judith A
authors.initials: JA
authors.lastname: Hoyland
authors.forename: James A
authors.initials: JA
authors.lastname: Covington
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Dove
mesh_terms: D000179:Acrylates / Q000008:administration & dosage / Q000737:chemistry*; D000328:Adult; D000368:Aged; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D017076:Computer-Aided Design; D004285:Dogs; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000502:physiology*; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000737:chemistry*; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*
chemical_list: D000179:Acrylates; D011092:Polyethylene Glycols; C036658:acrylic acid