title: Azadirachta indica (neem) leaf dietary effects on the immunity response and disease resistance of Asian seabass, Lates calcarifer challenged with Vibrio harveyi.
abstract: The present study was aimed to address the possible evaluation of Azadirachta indica (neem) leaf-supplemented diets on innate immune response in Asian seabass, Lates calcarifer fingerlings against Vibrio harveyi infection. Fish were fed for two weeks diets containing six graded levels of neem leaf at 0 g, 1 g, 2 g, 3 g, 4 g and 5 g per kg feed. Fish fed neem leaf-supplemented diet displayed significant differences (p < 0.05) in weight gain, specific growth rate (SGR) and feed conversion ratio (FCR) compared to the control group fed without neem leaf-supplemented diet. Various innate immune parameters were examined pre-challenge and post-challenge. Fish was injected intraperitoneally with a lethal dose of V. harveyi containing 10(8) cells mL(-1). Supplementation of neem leaf diet significantly increased phagocytic activity, superoxide anion production, serum lysozyme, serum bactericidal activity, serum anti-protease activity throughout the experimental period when compared with the control group. Dietary doses of neem leaf diet significantly influenced the immune parameters, haematological parameters and blood biochemical indices of treated fish. The results suggested that fish fed neem leaf-supplemented diet improved the immune system and increased survival rate in L. calcarifer fingerlings against V. harveyi infection.
authors.affiliation: Department of Fisheries, Government of Sindh, Pakistan. mrtalpur@yahoo.com
authors.forename: Allah Dad
authors.initials: AD
authors.lastname: Talpur
authors.forename: Mhd
authors.initials: M
authors.lastname: Ikhwanuddin
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D017756:Aquaculture; D031601:Azadirachta / Q000737:chemistry*; D001492:Bass / Q000254:growth & development / Q000276:immunology*; D001774:Blood Chemical Analysis; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D060467:Disease Resistance; D029941:Fish Proteins / Q000378:metabolism; D007113:Immunity, Innate; D009113:Muramidase / Q000378:metabolism; D010587:Phagocytosis; D010936:Plant Extracts / Q000008:administration & dosage*; D018515:Plant Leaves / Q000737:chemistry; D011480:Protease Inhibitors / Q000378:metabolism; D011897:Random Allocation; D013481:Superoxides / Q000378:metabolism; D014733:Vibrio / Q000502:physiology; D014735:Vibrio Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary
chemical_list: D029941:Fish Proteins; D010936:Plant Extracts; D011480:Protease Inhibitors; D013481:Superoxides; D009113:Muramidase