title: Sequencing of virulence genes shows limited genetic variability in Yersinia pseudotuberculosis.
abstract: Yersinia pseudotuberculosis is a foodborne pathogen often detected and identified using polymerase chain reaction (PCR) with primers targeted to virulence genes. Sequence variability of the virulence genes in strains representing different serotypes is unknown. Sequence variability could hinder the recognition of this pathogen by PCR and affect the host-pathogen interactions. Sequencing of inv, virF, and yadA of 18 Y. pseudotuberculosis strains showed limited variability of inv and virF, whereas the sequences of yadA varied considerably.
authors.affiliation: Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland. eveliina.palonen@helsinki.fi
authors.forename: Eveliina
authors.initials: E
authors.lastname: Palonen
authors.forename: Susanna
authors.initials: S
authors.lastname: Kangas
authors.forename: Panu
authors.initials: P
authors.lastname: Somervuo
authors.forename: Miia
authors.initials: M
authors.lastname: Lindström
authors.forename: Maria
authors.initials: M
authors.lastname: Fredriksson-Ahomaa
authors.forename: Mikael
authors.initials: M
authors.lastname: Skurnik
authors.forename: Hannu
authors.initials: H
authors.lastname: Korkeala
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005517:Foodborne Diseases / Q000382:microbiology*; D014644:Genetic Variation / Q000235:genetics*; D054884:Host-Pathogen Interactions; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D037521:Virulence Factors / Q000737:chemistry / Q000235:genetics*; D015011:Yersinia pseudotuberculosis / Q000235:genetics* / Q000472:pathogenicity; D015012:Yersinia pseudotuberculosis Infections / Q000382:microbiology*
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D037521:Virulence Factors; C064832:YadA protein, Yersinia