title: [Assessment of the effect of selected mixture of food additives on the protein metabolism--model studies].
abstract: BACKGROUND
  Contemporarily, food production without food additives is very rare. Increasingly often, however, scientific works report on adverse effects of specified, single food additives on the body. Data is, in turn, lacking on the synergistic effect of a mixture of different food additives on body functions and its main metabolic pathways.
  
  
  OBJECTIVE
  The objective of this study, an animal model, was to evaluate if and in what way the compound of chosen and most frequently used and consumed food additives, along with the change of diet composition to processed, purified, influence the selected markers of protein metabolism.
  
  
  METHODS
  The animals were divided into four groups, which were fed with compound of feed pellets: group I and II with basic compound, group III and IV with modified compound in which part of the full grain was replaced by isocalorie wheat flour type 500 and saccharose. Animals from groups I and III received tap water, which was standing for some time, to drink. Animals from groups II and IV received solution of chosen additives to food and next they were given water to drink. The amount of given food additives was evaluated by taking into consideration their consumption by people recalculated to 1 kg of their body mass. The experiment spanned for 7 weeks.
  
  
  RESULTS
  It was ascertained that the applied additives caused significant changes in total protein concentration and its fractions: albumin, alpha1-globulin, alpha2-globulin, beta-globulin and gamma-globulin in the blood serum of the animals under research, which can indicate and contribute to disclosure of creation of undesirable food reaction, especially when recommended levels of consumption of those additives are being exceeded. The organism response to the applied additives and accompanying it change of diet was essentially connected to sex of the animals. Undesirable character of changes taking place under the influence of applied additives, was observed both in animals fed with basic feed and modified feed with various intensity according to the parameter under research.
  
  
  CONCLUSIONS
  The analysis of the results achieved enabled concluding that the applied mixture of food additives caused significant changes in the concentration of total protein and its fractions: albumins, alphal-, alpha2-, beta- and gamma-globulins in blood serum of the investigated animals. These changes may indicate but also may contribute to the development or manifestation of undesirable nutritional responses, especially when recommended dietary allowances are exceeded. The body's response to the applied additives and concomitant modification of diet composition was significantly correlated with sex of the animals. The unfavorable character of changes following the administration of additives was observed in both the animals on the basal diet and these fed the modified feed mixture, yet with a different intensity that was found to depend not on the feeding group but on the parameter examined.
authors.affiliation: Zakład Fizjologii Zywienia Człowieka, Wydział Nauk o Zywności i Rybactwa, Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Mariola.Friedrich@zut.edu.pl
authors.forename: Mariola
authors.initials: M
authors.lastname: Friedrich
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kuchlewska
mesh_terms: D000418:Albumins / Q000187:drug effects / Q000378:metabolism; D000510:Alpha-Globulins / Q000187:drug effects / Q000378:metabolism; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001609:Beta-Globulins / Q000187:drug effects / Q000378:metabolism; D001798:Blood Proteins / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005503:Food Additives / Q000008:administration & dosage*; D008297:Male; D023421:Models, Animal; D018673:Nutrition Policy; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D005719:gamma-Globulins / Q000187:drug effects / Q000378:metabolism
chemical_list: D000418:Albumins; D000510:Alpha-Globulins; D001609:Beta-Globulins; D001798:Blood Proteins; D005503:Food Additives; D005719:gamma-Globulins