title: Electrophysiological and behavioral responses of male fall webworm moths (Hyphantria cunea) to Herbivory-induced mulberry (Morus alba) leaf volatiles.
abstract: Volatile organic compounds (VOCs) were collected from damaged and intact mulberry leaves (Morus alba L., Moraceae) and from Hyphantria cunea larvae by headspace absorption with Super Q columns. We identified their constituents using gas chromatography-mass spectrometry, and evaluated the responses of male H. cunea antennae to the compounds using gas chromatography-flame ionization detection coupled with electroantennographic detection. Eleven VOC constituents were found to stimulate antennae of male H. cunea moths: β-ocimene, hexanal, cis-3-hexenal, limonene, trans-2-hexenal, cyclohexanone, cis-2-penten-1-ol, 6-methyl-5-hepten-2-one, 4-hydroxy-4-methyl-2-pentanone, trans-3-hexen-1-ol, and 2,4-dimethyl-3-pentanol. Nine of these chemicals were released by intact, mechanically-damaged, and herbivore-damaged leaves, while cis-2-penten-1-ol was released only by intact and mechanically-damaged leaves and β-ocimene was released only by herbivore-damaged leaves. Results from wind tunnel experiments conducted with volatile components indicated that male moths were significantly more attracted to herbivory-induced volatiles than the solvent control. Furthermore, male moths' attraction to a sex pheromone lure was increased by herbivory-induced compounds and β-ocimene, but reduced by cis-2-penten-1-ol. A proof long-range field trapping experiment showed that the efficiency of sex pheromone lures in trapping male moths was increased by β-ocimene and reduced by cis-2-penten-1-ol.
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Tang
authors.forename: Jin Ping
authors.initials: JP
authors.lastname: Zhang
authors.forename: Zhong Ning
authors.initials: ZN
authors.lastname: Zhang
mesh_terms: D000080462:Acyclic Monoterpenes; D000475:Alkenes; D000818:Animals; D057506:Arthropod Antennae / Q000187:drug effects; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology*; D002633:Chemotaxis / Q000187:drug effects / Q000502:physiology*; D005410:Flame Ionization; D008401:Gas Chromatography-Mass Spectrometry; D060434:Herbivory / Q000502:physiology*; D008297:Male; D031625:Morus / Q000737:chemistry*; D009036:Moths / Q000502:physiology*; D018515:Plant Leaves / Q000737:chemistry*; D013045:Species Specificity; D013268:Stimulation, Chemical; D055549:Volatile Organic Compounds / Q000032:analysis / Q000494:pharmacology*
chemical_list: D000080462:Acyclic Monoterpenes; D000475:Alkenes; D055549:Volatile Organic Compounds; C443996:beta-ocimene