title: Quantifying the activity of cis-regulatory elements in the mouse retina by explant electroporation.
abstract: Transcription factors control gene expression by binding to noncoding regions of DNA known as -cis-regulatory elements (CREs; i.e., enhancer/promoters). Traditionally, cis-regulatory analysis has been carried out via mouse transgenesis which is time-consuming and nonquantitative. Electroporation of DNA reporter constructs into living mouse tissue is a rapid and effective alternative to transgenesis which permits quantitative assessment of cis-regulatory activity. Here, we present a simple technique for quantifying the activity of photoreceptor-specific CREs in living explanted mouse retinas.
authors.affiliation: Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA.
authors.forename: Cynthia L
authors.initials: CL
authors.lastname: Montana
authors.forename: Connie A
authors.initials: CA
authors.lastname: Myers
authors.forename: Joseph C
authors.initials: JC
authors.lastname: Corbo
mesh_terms: D000818:Animals; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D004210:Dissection / Q000379:methods; D018274:Electroporation / Q000379:methods*; D017930:Genes, Reporter; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D012045:Regulatory Sequences, Nucleic Acid; D012160:Retina / Q000378:metabolism*; D046509:Tissue Culture Techniques / Q000379:methods; D016707:Tissue Fixation / Q000379:methods
chemical_list: D004247:DNA