title: Zinc-substituted hydroxyapatite: a biomaterial with enhanced bioactivity and antibacterial properties.
abstract: Hydroxyapatite (HA) is a synthetic biomaterial and has been found to promote new bone formation when implanted in a bone defect site. However, its use is often limited due to its slow osteointegration rate and low antibacterial activity, particularly where HA has to be used for long term biomedical applications. This work will describe the synthesis and detailed characterization of zinc-substituted HA (ZnHA) as an alternative biomaterial to HA. ZnHA containing 1.6 wt% Zn was synthesized via a co-precipitation reaction between calcium hydroxide, orthophosphoric acid and zinc nitrate hexahydrate. Single-phase ZnHA particles with a rod-like morphology measuring ~50 nm in length and ~15 nm in width, were obtained and characterized using transmission electron microscopy and X-ray diffraction. The substitution of Zn into HA resulted in a decrease in both the a- and c-axes of the unit cell parameters, thereby causing the HA crystal structure to alter. In vitro cell culture work showed that ZnHA possessed enhanced bioactivity since an increase in the growth of human adipose-derived mesenchymal stem cells along with the bone cell differentiation markers, were observed. In addition, antibacterial work demonstrated that ZnHA exhibited antimicrobial capability since there was a significant decrease in the number of viable Staphylococcus aureus bacteria after in contact with ZnHA.
authors.affiliation: Department of Mechanical Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore, 117 576, Singapore. mpetes@nus.edu.sg
authors.forename: E S
authors.initials: ES
authors.lastname: Thian
authors.forename: T
authors.initials: T
authors.lastname: Konishi
authors.forename: Y
authors.initials: Y
authors.lastname: Kawanobe
authors.forename: P N
authors.initials: PN
authors.lastname: Lim
authors.forename: C
authors.initials: C
authors.lastname: Choong
authors.forename: B
authors.initials: B
authors.lastname: Ho
authors.forename: M
authors.initials: M
authors.lastname: Aizawa
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D055598:Chemical Phenomena / Q000187:drug effects; D017886:Durapatite / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000502:physiology; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D015032:Zinc / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D017886:Durapatite; D015032:Zinc