title: [Cellular HIV DNA quantitative testing and its significance in Chinese AIDS patients during 48 weeks' highly-active antiretroviral treatment].
abstract: OBJECTIVE
  To observe the dynamic changes of peripheral blood T lymphocytes and monocytes, which serve as HIV-1 viral reservoirs, in Chinese HIV-infected patients receiving highly-active antiretroviral treatment (HAART) for 48 weeks and its clinical significance.
  
  
  METHODS
  A total of 35 chronic HIV-1 infected adults initial received HAART. The peripheral blood T lymphocyte subsets counts were determined by flux cytometry at week 0, 24 and 48. Magnetic activated cell sorting was used to extract cellular DNA from monocytes and T lymphocytes purified from peripheral blood mononuclear cells. Real-time fluorescent quantitative PCR was used to detect the serum HIV RNA and HIV DNA of monocytes and T lymphocytes. SPSS 18.0 software was used to analyze the collected data.
  
  
  RESULTS
  At week 0, 24, and 48 after initiation of HAART, HIV RNA levels of peripheral blood were (4.12 ± 1.41), ≤ 1.69, and ≤ 1.69 lg copies/ml, respectively; CD(4)(+) T cells were (196 ± 101), (321.90 ± 112) and (392 ± 127) cells/µl, respectively; HIV DNA level in T lymphocytes were (4.03 ± 0.53), (2.74 ± 1.16) and (2.45 ± 0.41) lg copies/10(6) cells respectively; while in monocytes, HIV DNA levels were (2.51 ± 0.68), (2.16 ± 0.34)and (2.03 ± 0.25)lg copies/10(6) cells. Statistical analysis revealed that HIV RNA level was negatively correlated with the CD(4)(+) T cell count through the whole trail, while positively correlated with the HIV DNA level in blood T lymphocytes and monocytes. HIV DNA level in T lymphocytes decreased more slowly than HIV DNA in monocytes. Moreover, peripheral blood CD(4)(+) T cell count was negatively associated with the HIV DNA capacity from T lymphocytes.
  
  
  CONCLUSIONS
  Both T lymphocyte and monocyte may serve as viral reservoirs, and T lymphocyte might play a more important role as HIV reservoirs. The blood HIV RNA is correlated positively with the cellular HIV DNA, whereas, CD(4)(+) T cell count is correlated negatively with HIV DNA from lymphocytes, which suggests that HIV DNA levels in T lymphocyte might be one of indicators of AIDS progress during HAART.
authors.affiliation: Department of Infectious Diseases, Central South University, Changsha, China. yhzyhz2006@yahoo.com.cn
authors.forename: Yu-huang
authors.initials: YH
authors.lastname: Zheng
authors.forename: Diallo Mamadou
authors.initials: DM
authors.lastname: Alius
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: He
authors.forename: Bo
authors.initials: B
authors.lastname: He
authors.forename: Mei
authors.initials: M
authors.lastname: He
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Wang
authors.forename: Shui-ling
authors.initials: SL
authors.lastname: Xiao
authors.forename: Yan
authors.initials: Y
authors.lastname: Luo
authors.forename: Yun-hai
authors.initials: YH
authors.lastname: Yao
authors.forename: Yi-he
authors.initials: YH
authors.lastname: Lin
authors.forename: Zi
authors.initials: Z
authors.lastname: Chen
authors.forename: Hua-ying
authors.initials: HY
authors.lastname: Zhou
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000188:drug therapy* / Q000821:virology*; D000328:Adult; D023241:Antiretroviral Therapy, Highly Active; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000821:virology; D012367:RNA, Viral / Q000097:blood; D013601:T-Lymphocytes / Q000821:virology; D019562:Viral Load; D055815:Young Adult
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral