title: The oxidized low-density lipoprotein/β2-glycoprotein I complex is associated with abdominal obesity in healthy middle-aged men.
abstract: OBJECTIVE
  Oxidized low-density lipoprotein (oxLDL) in complex with β2-glycoprotein I (β2GPI) has been associated with autoimmune diseases, diabetes mellitus, chronic renal disease and coronary atherosclerosis. The aim of our study was to determine whether plasma levels of oxLDL/β2GPI complexes are associated with insulin resistance, inflammation and markers of endothelial damage in obese middle-aged men and, if so, whether oxLDL/β2GPI correlates better with insulin resistance parameters than oxLDL, advanced oxidation protein products (AOPP) or thioredoxin.
  
  
  METHODS
  A total of 72 healthy men were recruited (41 obese and 31 nonobese individuals). Waist circumference >94 cm was used as the criterion for abdominal obesity.
  
  
  RESULTS
  The obese men demonstrated higher oxLDL/β2GPI levels (p < 0.001), homeostasis model assessment of insulin resistance (p < 0.01) and intima-media thickness of the common carotid artery (p < 0.01). oxLDL/β2GPI correlated with more insulin resistance parameters compared to AOPP, thioredoxin or oxLDL. Furthermore, oxLDL/β2GPI was associated with plasminogen activator inhibitor-I (PAI-I; r = 0.365, p < 0.001) and negatively with interleukin-8 (r = -0.297, p < 0.05).
  
  
  CONCLUSIONS
  In summary, oxLDL/β2GPI reflects the criterion for abdominal obesity and markers of insulin resistance in our study. The independent positive correlation with PAI-I indicates that oxLDL/β2GPI may serve as an early marker of low-grade inflammation and atherosclerosis initiation.
authors.affiliation: Second Department of Internal Medicine, The Third Faculty of Medicine, Charles University Prague, Prague, Czech Republic. Kraml@fnkv.cz
authors.forename: Pavel J
authors.initials: PJ
authors.lastname: Kraml
authors.forename: Petr
authors.initials: P
authors.lastname: Syrovátka
authors.forename: Jana
authors.initials: J
authors.lastname: Potočková
authors.forename: Michal
authors.initials: M
authors.lastname: Anděl
mesh_terms: D000328:Adult; D062107:Advanced Oxidation Protein Products / Q000097:blood; D050197:Atherosclerosis / Q000097:blood / Q000503:physiopathology; D015415:Biomarkers / Q000097:blood; D059168:Carotid Intima-Media Thickness; D016022:Case-Control Studies; D006801:Humans; D007333:Insulin Resistance; D016209:Interleukin-8 / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D056128:Obesity, Abdominal / Q000097:blood* / Q000503:physiopathology; D018384:Oxidative Stress; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood; D012044:Regression Analysis; D013879:Thioredoxins / Q000097:blood; D055105:Waist Circumference; D053482:beta 2-Glycoprotein I / Q000097:blood*
chemical_list: D062107:Advanced Oxidation Protein Products; D015415:Biomarkers; D016209:Interleukin-8; D008077:Lipoproteins, LDL; D017395:Plasminogen Activator Inhibitor 1; C051321:SERPINE1 protein, human; D053482:beta 2-Glycoprotein I; C099252:oxidized low density lipoprotein; D013879:Thioredoxins