title: The C-C motif chemokine ligands CCL5, CCL11, and CCL24 induce the migration of circulating fibrocytes from patients with severe asthma.
abstract: The C-C motif chemokine ligand 5 (CCL5), CCL11, and CCL24 are involved in the pathogenesis of asthma, and their function is mainly associated with the airway recruitment of eosinophils. This study tested their ability to induce the migration of circulating fibrocytes, which may contribute to the development of irreversible airflow obstruction in severe asthma. The sputum fluid phase (SFP) from patients with severe/treatment-refractory asthma (PwSA) contained elevated concentrations of CCL5, CCL11, and CCL24 in comparison with the SFP from patients with non-severe/treatment-responsive asthma (PwNSA). The circulating fibrocytes from PwSA expressed the receptors for these chemokines at increased levels and migrated in response to recombinant CCL5, CCL11, and CCL24. The SFP from PwSA induced the migration of autologous fibrocytes, and its activity was significantly attenuated by neutralization of endogenous CCL5, CCL11, and CCL24. These findings suggest that CCL5, CCL11, and CCL24 may contribute to the airway recruitment of fibrocytes in severe asthma.
authors.affiliation: Avail Biomedical Research Institute, Basel, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Isgrò
authors.forename: L
authors.initials: L
authors.lastname: Bianchetti
authors.forename: M A
authors.initials: MA
authors.lastname: Marini
authors.forename: A
authors.initials: A
authors.lastname: Bellini
authors.forename: M
authors.initials: M
authors.lastname: Schmidt
authors.forename: S
authors.initials: S
authors.lastname: Mattoli
mesh_terms: D000199:Actins / Q000378:metabolism; D000328:Adult; D001249:Asthma / Q000276:immunology* / Q000378:metabolism; D001854:Bone Marrow Cells / Q000187:drug effects / Q000276:immunology; D002465:Cell Movement / Q000187:drug effects* / Q000276:immunology; D054413:Chemokine CCL11 / Q000494:pharmacology*; D054423:Chemokine CCL24 / Q000494:pharmacology*; D018946:Chemokine CCL5 / Q000494:pharmacology*; D002633:Chemotaxis / Q000187:drug effects / Q000276:immunology; D024061:Collagen Type III / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000276:immunology; D008875:Middle Aged; D054397:Receptors, CCR3 / Q000378:metabolism; D019713:Receptors, CCR5 / Q000378:metabolism; D013183:Sputum / Q000276:immunology
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; D054413:Chemokine CCL11; D054423:Chemokine CCL24; D018946:Chemokine CCL5; D024061:Collagen Type III; D054397:Receptors, CCR3; D019713:Receptors, CCR5