title: Nonenzymatic polymerization of ubiquitin: single-step synthesis and isolation of discrete ubiquitin oligomers.
abstract: Linked: a method based on thiol-ene chemistry enables the synthesis and purification of ubiquitin oligomers with ≥4 units. This approach, which employs free-radical polymerization, can be applied towards the synthesis of homogeneous Lys6-linked ubiquitin oligomers currently inaccessible by enzymatic methods. By using these chains, one can study their roles in the ubiquitin proteasome system and the DNA damage response pathway.
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison, 53706, USA.
authors.forename: Vivian H
authors.initials: VH
authors.lastname: Trang
authors.forename: Ellen M
authors.initials: EM
authors.lastname: Valkevich
authors.forename: Shoko
authors.initials: S
authors.lastname: Minami
authors.forename: Yi-Chen
authors.initials: YC
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Ge
authors.forename: Eric R
authors.initials: ER
authors.lastname: Strieter
mesh_terms: D006801:Humans; D058105:Polymerization; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000737:chemistry; D025801:Ubiquitin / Q000378:metabolism*
chemical_list: C085834:PA700 proteasome activator; D013438:Sulfhydryl Compounds; D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex