title: Protective effect of thymoquinone against cisplatin-induced ototoxicity.
abstract: The aim of this study was to investigate the potential protective effect of thymoquinone against cisplatin-induced ototoxicity. This study is a prospective, controlled experimental animal study. Experiments were performed on 30 healthy female Sprague-Dawley rats. Thirty animals were divided into three groups of 10 animals each. Group 1 received an intraperitoneal (i.p.) injection of cisplatin 15 mg/kg. Group 2 received i.p. thymoquinone 40 mg/kg/day for 2 days prior to cisplatin injection and third day i.p. cisplatin 15 mg/kg was administered concomitantly. Group 2 continued to receive i.p. thymoquinone until fifth day. Group 3 received i.p. thymoquinone 40 mg/kg/day for 5 days. Pretreatment distortion product otoacoustic emissions (DPOAE) and auditory brain stem responses (ABR) testing from both ears were obtained from the animals in all groups. After the baseline measurements, drugs were injected intraperitonally. After an observation period of 3 days, DPOAE measurements and ABR testing were obtained again and compared with the pretreatment values. There was no statistically significant difference between pre and post-treatment DPOAE responses and ABR thresholds group 2 and 3. However, group 1 demonstrated significant deterioration of the ABR thresholds and DPOAE responses. Our results suggest that DPOAE responses and ABR thresholds were preserved in the cisplatin plus TQ-treated group when compared with the group receiving cisplatin alone. According to these results, cisplatin-induced ototoxicity may be prevented by thymoquinone use in rats.
authors.affiliation: Department of ENT, Kayseri Training and Research Hospital, Kayseri, Turkey. musagit@yahoo.com
authors.forename: Mustafa
authors.initials: M
authors.lastname: Sagit
authors.forename: Ferhat
authors.initials: F
authors.lastname: Korkmaz
authors.forename: Alper
authors.initials: A
authors.lastname: Akcadag
authors.forename: Mehmet Akıf
authors.initials: MA
authors.lastname: Somdas
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000970:Antineoplastic Agents / Q000009:adverse effects*; D000975:Antioxidants / Q000627:therapeutic use*; D016227:Benzoquinones / Q000627:therapeutic use*; D002945:Cisplatin / Q000009:adverse effects*; D003051:Cochlea / Q000187:drug effects*; D016057:Evoked Potentials, Auditory, Brain Stem; D005260:Female; D034381:Hearing Loss / Q000139:chemically induced / Q000517:prevention & control*; D017084:Otoacoustic Emissions, Spontaneous; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D016227:Benzoquinones; C003466:thymoquinone; D002945:Cisplatin