title: Prevention of amyloid-β fibril formation using antibodies against the C-terminal region of amyloid-β1-40 and amyloid-β1-42.
abstract: Alzheimer's disease is characterized by the abnormal aggregation of amyloid-β (Aβ)1-40 and Aβ1-42 peptides into fibrils. In this work, we analyzed the kinetics of Aβ1-40 and Aβ1-42 fibril formation in vitro using Thioflavin T fluorescence. We synthesized high-purity peptides and performed a hexafluoro-2-propanol pre-treatment to yield uniform peptide solutions as starting materials. We found that the aggregation is clearly affected by the presence of sub-millimolar quantities of antibodies against the C-terminal region of the peptides. Because the fibrillization of these peptides is closely related to the pathogenesis of Alzheimer's disease, blocking this process may provide significant therapeutic benefit.
authors.affiliation: Araclon Biotech, CIBIR Laboratory, Logroño, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Montañés
authors.forename: Diego
authors.initials: D
authors.lastname: Casabona
authors.forename: Leticia
authors.initials: L
authors.lastname: Sarasa
authors.forename: Pedro
authors.initials: P
authors.lastname: Pesini
authors.forename: Manuel
authors.initials: M
authors.lastname: Sarasa
mesh_terms: D000682:Amyloid / Q000187:drug effects* / Q000648:ultrastructure; D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000276:immunology* / Q000494:pharmacology; D000906:Antibodies / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D066298:In Vitro Techniques; D046529:Microscopy, Electron, Transmission; D010446:Peptide Fragments / Q000737:chemistry* / Q000276:immunology* / Q000494:pharmacology; D020005:Propanols; D013997:Time Factors; D006845:Hydrocarbons, Fluorinated
chemical_list: D000682:Amyloid; D016229:Amyloid beta-Peptides; D000906:Antibodies; D010446:Peptide Fragments; D020005:Propanols; C078592:amyloid beta-protein (1-40); C075222:amyloid beta-protein (1-42); C001337:hexafluoroisopropanol; D006845:Hydrocarbons, Fluorinated