title: Ammonificins C and D, hydroxyethylamine chromene derivatives from a cultured marine hydrothermal vent bacterium, Thermovibrio ammonificans.
abstract: Chemical and biological investigation of the cultured marine hydrothermal vent bacterium, Thermovibrio ammonifican led to the isolation of two hydroxyethylamine chromene derivatives, ammonificins C and D. Their structures were elucidated using combination of NMR and mass spectrometry. Absolute stereochemistry was ascertained by comparison of experimental and calculated CD spectra. Biological evaluation and assessment were determined using the patented ApopScreen cell-based screen for apoptosis-induction. Ammonificins C and D induce apoptosis in micromolar concentrations. To our knowledge, this finding is the first report of chemical compounds that induce apoptosis from the cultured deep-sea marine organism, hydrothermal vent bacterium, Thermovibrio ammonificans.
authors.affiliation: Center for Marine Biotechnology, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA.
authors.forename: Eric H
authors.initials: EH
authors.lastname: Andrianasolo
authors.affiliation: Center for Marine Biotechnology, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA.
authors.forename: Liti
authors.initials: L
authors.lastname: Haramaty
authors.affiliation: Center for Marine Biotechnology, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Rosario-Passapera
authors.affiliation: Center for Marine Biotechnology, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA.
authors.forename: Costantino
authors.initials: C
authors.lastname: Vetriani
authors.affiliation: Center for Marine Biotechnology, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Falkowski
authors.affiliation: Center for Advanced Biotechnology and Medicine, Department of Molecular Biology and Biochemistry, Rutgers, The State University of New Jersey, 679 Hoes Lane, Piscataway, NJ 08854, USA.
authors.forename: Eileen
authors.initials: E
authors.lastname: White
authors.affiliation: Center for Marine Biotechnology, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Lutz
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D001578:Benzopyrans / Q000737:chemistry* / Q000378:metabolism; D002942:Circular Dichroism; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001578:Benzopyrans; D010636:Phenols