title: Analysis of p53, K-ras gene mutation & Helicobacter pylori infection in patients with gastric cancer & peptic ulcer disease at a tertiary care hospital in north India.
abstract: OBJECTIVE
  Mutations in the oncogene and tumour suppressor genes play an important role in carcinogenesis. We investigated the association of p53 and K-ras gene mutation and Helicobacter pylori infection in patients with gastric cancer (GC) and peptic ulcer disease (PUD) attending a tertiary care hospital in north India.
  
  
  METHODS
  In total, 348 adult patients [62 GC, 45 PUD and 241 non-ulcer dyspepsia (NUD)] who underwent an upper gastrointestinal endoscopy were enrolled. H. pylori infection was diagnosed by rapid urease test, culture, histopathology and PCR. Mutation in the exon 5-8 of p53 gene was analyzed by PCR-single stranded conformational polymorphism (SSCP) and confirmed by sequence analysis. K-ras gene codon 12 mutation was analyzed by PCR-based restriction fragment length polymorphism.
  
  
  RESULTS
  Overall p53 gene mutation was found in 4.6 per cent of the study population, and its distribution in GC, PUD and NUD was 21, 4.4 and 0.4 per cent, respectively. p53 gene mutation was significantly higher in patients with GC than PUD (P<0.05) and NUD (P<0.001). No difference in p53 gene mutation was observed between H. pylori infected and non-infected individuals. K-ras gene mutation was absent in all the patients.
  
  
  CONCLUSIONS
  Our results show that p53 gene mutation may be associated with gastric carcinogenesis independent to H. pylori infection and absence of K-ras gene mutation questions its role in the pathogenesis of GC and PUD in Indian patients.
authors.affiliation: Department of Biochemistry & Molecular Biology, University of Texas Medical Branch, Texas, USA.
authors.forename: Ashish
authors.initials: A
authors.lastname: Saxena
authors.forename: Sanket Kumar
authors.initials: SK
authors.lastname: Shukla
authors.forename: Kashi Nath
authors.initials: KN
authors.lastname: Prasad
authors.forename: Uday Chand
authors.initials: UC
authors.lastname: Ghoshal
mesh_terms: D017931:DNA Primers / Q000235:genetics; D011905:Genes, ras / Q000235:genetics*; D016481:Helicobacter Infections / Q000235:genetics*; D016480:Helicobacter pylori; D006801:Humans; D009154:Mutation / Q000235:genetics; D010437:Peptic Ulcer / Q000235:genetics* / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA; D013274:Stomach Neoplasms / Q000235:genetics* / Q000382:microbiology*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D017931:DNA Primers; D016159:Tumor Suppressor Protein p53