title: MicroRNA-15a promotes neuroblastoma migration by targeting reversion-inducing cysteine-rich protein with Kazal motifs (RECK) and regulating matrix metalloproteinase-9 expression.
abstract: In this study, we found that the expression of miR-15a was positively correlated with neuroblastoma (NB) clinical pathological stage and was negatively correlated with reversion-inducing cysteine-rich protein with Kazal motifs (RECK) expression. Using the enhanced green fluorescent protein (EGFP) reporter construct carrying the 3'-UTR of RECK, we identified RECK as a direct target of miR-15a. Suppression of miR-15a significantly decreased the migration ability of GI-LA-N and SK-N-SH cell lines, whereas overexpression of miR-15a increased the migration ability; these effects could be partly reversed by RECK inhibition or ectopic expression. Moreover, inhibition of miR-15a significantly increased secreted matrix metalloproteinase-9 expression in culture medium through regulating the expression of RECK. These findings provide new insights into the characteristics of the miR-15a-RECK-matrix metalloproteinase-9 axis in NB progression, especially in NB migration and invasion.
authors.affiliation: Department of Pediatric Surgery, The Affiliated Hospital of Medical College Qingdao University, Qingdao, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Xin
authors.forename: Bao
authors.initials: B
authors.lastname: Buhe
authors.forename: Lu
authors.initials: L
authors.lastname: Hongting
authors.forename: Yang
authors.initials: Y
authors.lastname: Chuanmin
authors.forename: Hao
authors.initials: H
authors.lastname: Xiwei
authors.forename: Zhang
authors.initials: Z
authors.lastname: Hong
authors.forename: Han
authors.initials: H
authors.lastname: Lulu
authors.forename: Dong
authors.initials: D
authors.lastname: Qian
authors.forename: Wang
authors.initials: W
authors.lastname: Renjie
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D058851:GPI-Linked Proteins / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D008957:Models, Genetic; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D009447:Neuroblastoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013050:Spectrometry, Fluorescence
chemical_list: D020413:3' Untranslated Regions; D058851:GPI-Linked Proteins; C505361:MIRN15 microRNA, human; D035683:MicroRNAs; C115460:RECK protein, human; D049452:Green Fluorescent Proteins; D020780:Matrix Metalloproteinase 9