title: Micropropagation of Iris sp.
abstract: Irises are perennial plants widely used as ornamental garden plants or cut flowers. Some species accumulate secondary metabolites, making them highly valuable to the pharmaceutical and perfume industries. Micropropagation of irises has successfully been accomplished by culturing zygotic embryos, different flower parts, and leaf base tissues as starting explants. Plantlets are regenerated via somatic embryogenesis, organogenesis, or both processes at the same time depending on media composition and plant species. A large number of uniform plants are produced by somatic embryogenesis, however, some species have decreased morphogenetic potential overtime. Shoot cultures obtained by organogenesis can be multiplied for many years. Somatic embryogenic tissue can be reestablished from leaf bases of in vitro-grown shoots. The highest number of plants can be obtained by cell suspension cultures. This chapter describes effective in vitro plant regeneration protocols for Iris species from different types of explants by somatic embryogenesis and/or organogenesis suitable for the mass propagation of ornamental and pharmaceutical irises.
authors.affiliation: Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia. sladja@ibiss.bg.ac.rs
authors.forename: Slađana
authors.initials: S
authors.lastname: Jevremović
authors.forename: Zoran
authors.initials: Z
authors.lastname: Jeknić
authors.forename: Angelina
authors.initials: A
authors.lastname: Subotić
mesh_terms: D000064:Acclimatization; D003470:Culture Media / Q000737:chemistry; D046508:Culture Techniques / Q000379:methods*; D018525:Germination; D031323:Iris Plant / Q000254:growth & development* / Q000502:physiology; D018515:Plant Leaves / Q000196:embryology / Q000502:physiology; D018517:Plant Roots / Q000254:growth & development / Q000502:physiology; D012038:Regeneration; D012639:Seeds / Q000254:growth & development / Q000502:physiology; D013242:Sterilization
chemical_list: D003470:Culture Media