title: Recent advances in preparation and application of hybrid organic-silica monolithic capillary columns.
abstract: Hybrid organic-silica monolithic columns, regarded as a second generation of silica-based monoliths, have received much interest due to their unique properties over the pure silica-based monoliths. This review mainly focuses on development in the fields of preparation of hybrid monolithic columns in a capillary and their application for CEC and capillary liquid chromatography separation, as well as for sample pretreatment of solid-phase microextraction and immobilized enzyme reactor since July 2010. The preparation approaches are comprehensively summarized with three routes: (i) general sol-gel process using trialkoxysilanes and tetraalkoxysilanes as coprecursors; (ii) "one-pot" process of alkoxysilanes and organic monomers concomitantly proceeding sol-gel chemistry and free radical polymerization; and (iii) other polymerization approaches of organic monomers containing silanes. The modification of hybrid monoliths containing reactive groups to acquire the desired surface functionality is also described.
authors.affiliation: CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China. junjieou@dicp.ac.cn
authors.forename: Junjie
authors.initials: J
authors.lastname: Ou
authors.forename: Hui
authors.initials: H
authors.lastname: Lin
authors.forename: Zhenbin
authors.initials: Z
authors.lastname: Zhang
authors.forename: Guang
authors.initials: G
authors.lastname: Huang
authors.forename: Jing
authors.initials: J
authors.lastname: Dong
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
mesh_terms: D053801:Capillary Electrochromatography / Q000295:instrumentation* / Q000379:methods / Q000639:trends; D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods; D004800:Enzymes, Immobilized / Q000378:metabolism; D005782:Gels; D058105:Polymerization; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D052617:Solid Phase Microextraction; D013499:Surface Properties
chemical_list: D004800:Enzymes, Immobilized; D005782:Gels; D012821:Silanes; D012822:Silicon Dioxide