title: Antigenic and immunogenic properties of truncated VP28 protein of white spot syndrome virus in Procambarus clarkii.
abstract: Previous studies identify VP28 envelope protein of white spot syndrome virus (WSSV) as its main antigenic protein. Although implicated in viral infectivity, its functional role remains unclear. In the current study, we described the production of polyclonal antibodies to recombinant truncated VP28 proteins including deleted N-terminal (rVP28ΔN), C-terminal (rVP28ΔC) and middle (rVP28ΔM). In antigenicity assays, antibodies developed from VP28 truncations lacking the N-terminal or middle regions showed significantly lowered neutralization of WSSV in crayfish, Procambarus clarkii. Further immunogenicity analysis showed reduced relative percent survival (RPS) in crayfish vaccinating with these truncations before challenge with WSSV. These results indicated that N-terminal (residues 1-27) and middle region (residues 35-95) were essential to maintain the neutralizing linear epitopes of VP28 and responsible in eliciting immune response. Thus, it is most likely that these regions are exposed on VP28, and will be useful for rational design of effective vaccines targeting VP28 of WSSV.
authors.affiliation: Feed Science Institute, College of Animal Science, Zhejiang University, Hangzhou 310058, PR China. huahuadu@zju.edu.cn
authors.forename: Hua-Hua
authors.initials: HH
authors.lastname: Du
authors.forename: Chong-Lin
authors.initials: CL
authors.lastname: Hou
authors.forename: Xiao-Guo
authors.initials: XG
authors.lastname: Wu
authors.forename: Rong-hui
authors.initials: RH
authors.lastname: Xie
authors.forename: Yi-Zhen
authors.initials: YZ
authors.lastname: Wang
mesh_terms: D000818:Animals; D003400:Astacoidea / Q000276:immunology / Q000821:virology*; D004926:Escherichia coli / Q000235:genetics; D008968:Molecular Conformation; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D020539:Sequence Analysis, Protein; D017385:Sequence Homology; D019444:Vaccines, DNA / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*; D046848:White spot syndrome virus 1 / Q000235:genetics / Q000276:immunology*
chemical_list: D011994:Recombinant Proteins; C548473:VP28 protein, white spot syndrome virus; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014765:Viral Vaccines