title: Reverse transcription loop-mediated isothermal amplification for rapid detection of the newly emerged poultry Flavivirus.
abstract: Poultry Flavivirus (PF) was a recently emerged virus with high morbidity rates and mortality rates in China. It is the causative agent of egg drop syndrome at present. Development of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was the most efficient way to prevent and control the PF disease. The assay was performed at 64 °C for 45 min, using six specific primers that recognized eight targets of the PF E gene. The RT-LAMP assay, compared to conventional reverse transcription polymerase chain reaction, has 100-fold-greater sensitivity, with a detection limit of 1 × 10(-3) copies per μL RNA and no cross-reaction with poultry other viruses. The RT-LAMP assay is a valuable tool for detected PF without requiring any sophisticated equipment, and the detection has potential usefulness for clinical diagnosis in the field.
authors.affiliation: Department of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, Fuzhou, China. lizhaolong522@163.com
authors.forename: Zhaolong
authors.initials: Z
authors.lastname: Li
authors.forename: Shaoying
authors.initials: S
authors.lastname: Chen
authors.forename: Shao
authors.initials: S
authors.lastname: Wang
authors.forename: Shilong
authors.initials: S
authors.lastname: Chen
authors.forename: Fengqian
authors.initials: F
authors.lastname: Lin
mesh_terms: D000818:Animals; D002681:China; D017931:DNA Primers / Q000235:genetics; D005416:Flavivirus / Q000235:genetics / Q000302:isolation & purification*; D018177:Flavivirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D011200:Poultry; D011201:Poultry Diseases / Q000175:diagnosis* / Q000821:virology*; D012680:Sensitivity and Specificity; D014730:Veterinary Medicine / Q000379:methods*; D014759:Viral Envelope Proteins / Q000235:genetics; D014773:Virology / Q000379:methods
chemical_list: D017931:DNA Primers; D014759:Viral Envelope Proteins; C065696:glycoprotein E, Flavivirus