title: Expression of human endogenous retrovirus-K coincides with that of micro-RNA-663 and -638 in germ-cell tumor cells.
abstract: OBJECTIVE
  The cell line GH was established from germ-cell tumor tissue; human endogenous retrovirus-K (HERV-K) expression was detectable after prolonged culture of the cells, particularly in cells that formed domes and vesicles. In addition, keeping GH cells in culture at high cell densities increased HERV-K expression. Here, we studied whether this inducible HERV-K expression is accompanied by differences in microRNA (miRNA) expression patterns of GH cells.
  
  
  METHODS
  The global miRNA expression pattern of GH cell samples (HERV-K high versus low) was analyzed by miRNA arrays.
  
  
  RESULTS
  Two miRNAs were found to be differentially regulated and to exhibit expression parallel to that of HERV-K. The identified miRNAs-663 and -638, have been reported to be involved in multiple processes, including cellular senescence. However, induction of HERV-K expression did not change the cellular senescence status of GH cells.
  
  
  CONCLUSIONS
  The expression of these two miRNAs might be useful as novel diagnostic and prognostic markers in patients with tumors.
authors.affiliation: Paul-Ehrlich-Institute, Paul-Ehrlich Strasse 51-59, 63225 Langen, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Kraus
authors.forename: Bettina
authors.initials: B
authors.lastname: Mönk
authors.forename: Katja
authors.initials: K
authors.lastname: Sliva
authors.forename: Barbara S
authors.initials: BS
authors.lastname: Schnierle
mesh_terms: D015152:Blotting, Northern; D015153:Blotting, Western; D020077:Endogenous Retroviruses; D015686:Gene Products, env / Q000032:analysis / Q000096:biosynthesis; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009373:Neoplasms, Germ Cell and Embryonal / Q000235:genetics* / Q000821:virology*; D011379:Prognosis; D059467:Transcriptome
chemical_list: D015686:Gene Products, env; C552243:MIRN663 microRNA, human; D035683:MicroRNAs