title: Efficacy of clarithromycin on biofilm formation  of methicillin-resistant Staphylococcus pseudintermedius.
abstract: BACKGROUND
  Surgical site infections (SSIs) caused by biofilm-forming methicillin-resistant Staphylococcus pseudintermedius (MRSP) have emerged as the most common hospital-acquired infections in companion animals. No methods currently exist for the therapeutic remediation of SSIs caused by MRSP in biofilms. Clarithromycin (CLA) has been shown to prevent biofilm formation by Staphylococcus aureus. This study aims to assess the in vitro activity of CLA in eradicating MRSP biofilm formation on various materials.
  
  
  RESULTS
  Quantitative assay results (P = 0.5126) suggest that CLA does not eradicate MRSP biofilm formation on polystyrene after 4 - 24 h growth periods. Scanning electron micrographs confirmed that CLA did not eradicate MRSP biofilm formed on orthopaedic implants.
  
  
  CONCLUSIONS
  By determining the in vitro characteristics and activities of MRSP isolates alone and against antibiotics, in vitro models of biofilm related infections can be made. In vitro data suggests that CLA does not effectively eradicate S. pseudintermedius biofilms in therapeutic doses.
authors.affiliation: School of Engineering, University of Guelph, Ontario, Canada.
authors.forename: Matthew
authors.initials: M
authors.lastname: Dicicco
authors.forename: Suresh
authors.initials: S
authors.lastname: Neethirajan
authors.forename: Ameet
authors.initials: A
authors.lastname: Singh
authors.forename: J Scott
authors.initials: JS
authors.lastname: Weese
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D017291:Clarithromycin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D016106:Methicillin Resistance; D013210:Staphylococcus / Q000187:drug effects* / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D017291:Clarithromycin