title: Complete genome sequencing of a velogenic viscerotropic avian paramyxovirus 1 isolated from pheasants (Pucrasia macrolopha) in Lahore, Pakistan.
abstract: We report the complete genome sequence of avian paramyxovirus 1 (APMV-1) isolated from an acute and highly contagious outbreak in pheasants (Pucrasia macrolopha) in Lahore, Pakistan. Biological and serological characterization showed a velogenic strain of APMV-1, which was further confirmed by the sequence analysis of the cleavage site in the fusion protein. Complete genome sequencing and phylogenetic analysis indicated that this isolate belonged to genotype VII, specifically to subgenotype VIIa, and clustered closely with isolates characterized from Indonesia. Notably, the isolate showed significant differences from previously characterized APMV-1 from Pakistani commercial and rural chicken.
authors.affiliation: University Diagnostic Laboratory, University of Veterinary and Animal Sciences, Lahore, Pakistan. vetvains@uvas.edu.pk
authors.forename: Muhammad Zubair
authors.initials: MZ
authors.lastname: Shabbir
authors.forename: Mohsan Ullah
authors.initials: MU
authors.lastname: Goraya
authors.forename: Muhammad
authors.initials: M
authors.lastname: Abbas
authors.forename: Tahir
authors.initials: T
authors.lastname: Yaqub
authors.forename: Muhammad Abu Bakr
authors.initials: MA
authors.lastname: Shabbir
authors.forename: Arfan
authors.initials: A
authors.lastname: Ahmad
authors.forename: Muhammad
authors.initials: M
authors.lastname: Anees
authors.forename: Muhammad
authors.initials: M
authors.lastname: Munir
mesh_terms: D000818:Animals; D046094:Galliformes / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009522:Newcastle disease virus / Q000235:genetics*; D010154:Pakistan