title: Method validation and simultaneous determination of retinol, retinyl palmitate, β-carotene, α-tocopherol and vitamin C in rat serum treated with 7,12 dimethylbenz[a]anthracene and Plantago major L. by high- performance liquid chromatography using diode-array detection.
abstract: A new and simple high-performance liquid chromatography method was developed and validated for the simultaneous determination of retinol, retinyl palmitate, β-carotene, α-tocopherol and vitamin C in rat serum treated with Plantago Major L. and 7,12 dimethylbenz[a]anthracene. High-performance liquid chromatography analysis was performed utilizing an Inertsil ODS3 reversed phase column with methanol-tetrahydrofuran-water as mobile phase under gradient conditions, at 1.5 mL min(-1) flow rate and 25 °C. Diode-array detection was at 325, 450, 290 and 270 nm (retinol and retinyl palmitate), β-carotene, α-tocopherol and vitamin C, respectively and runnig time 18 min. The high-performance liquid chromatography assay and extraction procedure proposed are simple, rapid, sensitive and accurate. The method was then applied for the determination of retinol, retinyl palmitate, β-carotene, α-tocopherol and vitamin C in rat serum. Results of this study demonstrated that; at 60th day DMBA-treated group, there was a significant decrease in vitamin levels compared to the levels of control group. A significant increase was observed in vitamin levels of 7,12 dimethylbenz[α]anthracene+Plantago Major L.-treated group compared to the DMBA-treated group. Additionally, the results obtained in the study are found to be in agreement with data reported in the literature.
authors.affiliation: Batman University, Faculty of Arts and Sciences, Department of Analytical Chemistry, and Batman University, Health Services Vocational College, Batman, Turkey. leventkadir@hotmail.com
authors.forename: Abdulkadi
authors.initials: A
authors.lastname: Levent
authors.forename: Gokhan
authors.initials: G
authors.lastname: Oto
authors.forename: Suat
authors.initials: S
authors.lastname: Ekin
authors.forename: Ismet
authors.initials: I
authors.lastname: Berber
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000008:administration & dosage / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000097:blood*; D002273:Carcinogens / Q000008:administration & dosage / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D004224:Diterpenes; D010936:Plant Extracts / Q000008:administration & dosage / Q000302:isolation & purification / Q000378:metabolism; D010943:Plantago / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D000084562:Retinyl Esters; D012680:Sensitivity and Specificity; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000097:blood*; D024502:alpha-Tocopherol / Q000097:blood*; D019207:beta Carotene / Q000097:blood*
chemical_list: D002273:Carcinogens; D004224:Diterpenes; D010936:Plant Extracts; D000084562:Retinyl Esters; D019207:beta Carotene; D014801:Vitamin A; C014794:retinol palmitate; D015127:9,10-Dimethyl-1,2-benzanthracene; D024502:alpha-Tocopherol; D001205:Ascorbic Acid