title: A U-Box E3 ubiquitin ligase, PUB20, interacts with the Arabidopsis G-protein β subunit, AGB1.
abstract: An Arabidopsis U-box E3 ubiquitin ligase Plant U-box 20 (PUB20; alternatively called AtCMPG1) was identified as a possible interactor of the Arabidopsis G-protein β subunit, AGB1, by yeast two-hybrid screening. A bimolecular fluorescence complementation (BiFC) assay showed that PUB20 interacted with AGB1 in the nuclei and the cytosol. The expression levels of PUB20 and its closest homolog, PUB21 were stable under many conditions. GUS driven by the PUB20 promoter was active in anthers, pollen, premature seeds and receptacles and GUS driven by the PUB21 promoter was active in anthers and funiculi. PUB20 was found to have autoubiquitination activity in vitro.
authors.affiliation: Asian Natural Environmental Science Center (ANESC), The University of Tokyo, Tokyo, Japan.
authors.forename: Shio
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Daisuke
authors.initials: D
authors.lastname: Tsugama
authors.forename: Shenkui
authors.initials: S
authors.lastname: Liu
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Takano
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D044387:GTP-Binding Protein beta Subunits / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020798:Two-Hybrid System Techniques; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: C089590:AGB1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D017931:DNA Primers; D044387:GTP-Binding Protein beta Subunits; D044767:Ubiquitin-Protein Ligases