title: A germline clone screen on the X chromosome reveals novel meiotic mutants in Drosophila melanogaster.
abstract: In an effort to isolate novel meiotic mutants that are severely defective in chromosome segregation and/or exchange, we employed a germline clone screen of the X chromosome of Drosophila melanogaster. We screened over 120,000 EMS-mutagenized chromosomes and isolated 19 mutants, which comprised nine complementation groups. Four of these complementation groups mapped to known meiotic genes, including mei-217, mei-218, mei-9, and nod. Importantly, we have identified two novel complementation groups with strong meiotic phenotypes, as assayed by X chromosome nondisjunction. One complementation group is defined by three alleles, and the second novel complementation group is defined by a single allele. All 19 mutants are homozygous viable, fertile, and fully recessive. Of the 9 mutants that have been molecularly characterized, 5 are canonical EMS-induced transitions, and the remaining 4 are transversions. In sum, we have identified two new genes that are defined by novel meiotic mutants, in addition to isolating new alleles of mei-217, mei-218, mei-9, and nod.
authors.affiliation: Stowers Institute for Medical Research, 1000 E. 50th Street, Kansas City, MO 64110, USA.
authors.forename: Kimberly A
authors.initials: KA
authors.lastname: Collins
authors.forename: Jonathon G
authors.initials: JG
authors.lastname: Callicoat
authors.forename: Cathleen M
authors.initials: CM
authors.lastname: Lake
authors.forename: Cailey M
authors.initials: CM
authors.lastname: McClurken
authors.forename: Kathryn P
authors.initials: KP
authors.lastname: Kohl
authors.forename: R Scott
authors.initials: RS
authors.lastname: Hawley
mesh_terms: D000483:Alleles; D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics; D020090:Chromosome Segregation / Q000235:genetics; D059006:Chromosomes, Insect / Q000235:genetics*; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics; D005808:Genes, Recessive; D005816:Genetic Complementation Test; D005820:Genetic Testing; D018095:Germ-Line Mutation; D006720:Homozygote; D016547:Kinesins / Q000235:genetics; D008540:Meiosis / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics; D010641:Phenotype; D014960:X Chromosome / Q000235:genetics*
chemical_list: D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; C097527:MEI-9 protein, Drosophila; D009687:Nuclear Proteins; C103176:mei-218 protein, Drosophila; C080298:Nod protein, Drosophila; D016547:Kinesins