title: ECHS1 acts as a novel HBsAg-binding protein enhancing apoptosis through the mitochondrial pathway in HepG2 cells.
abstract: We aimed to confirm the role of ECHS1 as a binding protein of HBsAg (HBs) and investigate its function during the development of hepatocellular carcinoma (HCC). Our results show that both exogenous and endogenous ECHS1 proteins bind to HBs and co-localize in the cytoplasm in vitro. The coexistence of HBs and ECHS1 enhances HepG2 cell apoptosis, affects ECHS1 localization in the mitochondria and induces apoptosis by decreasing the mitochondrial membrane potential (MMP). These findings suggest that ECHS1 may be applied as a potential therapeutic target during the treatment of HBV-related hepatitis or HCC.
authors.affiliation: Department of Gastroenterology, Zhongshan Hospital Affiliated to Xiamen University, 201 Hubin South Road, Xiamen 361004, Fujian Province, China.
authors.forename: Chuan-Xing
authors.initials: CX
authors.lastname: Xiao
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Yang
authors.forename: Qing-Wen
authors.initials: QW
authors.lastname: Huang
authors.forename: Yu-Qin
authors.initials: YQ
authors.lastname: Zhang
authors.forename: Bi-Yun
authors.initials: BY
authors.lastname: Lin
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Liu
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Liu
authors.forename: Amarsanaa
authors.initials: A
authors.lastname: Jazag
authors.forename: Bayasi
authors.initials: B
authors.lastname: Guleng
authors.forename: Jian-Lin
authors.initials: JL
authors.lastname: Ren
mesh_terms: D017209:Apoptosis / Q000502:physiology; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D004746:Enoyl-CoA Hydratase / Q000235:genetics / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D056945:Hep G2 Cells; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*; D053078:Membrane Potential, Mitochondrial / Q000502:physiology; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding; D014162:Transfection
chemical_list: D006514:Hepatitis B Surface Antigens; D004746:Enoyl-CoA Hydratase