title: Comparison of buffers for extraction of mite allergen der p 1 from dust.
abstract: Der p 1 is the main allergen of house dust mite Dermatophagoides pteronyssinus, which has routinely been detected in residential dust. However, the procedure for extracting Der p 1 from reservoir dust has not been well defined. The aim of this study was to compare Der p 1 mass fractions in dust extracts prepared using the following extraction buffers: phosphate (pH 7.4), borate (pH 8.0), and ammonium bicarbonate (pH 8.0), all with 0.05 % Tween 20. Twenty-eight dust samples were divided into three aliquots and each portion was extracted with one of the three buffers at room temperature. Der p 1 mass fractions were measured in a total of 84 dust extracts using the enzyme immunoassay (range: 0.1 μg g-1 to 7.53 μg g-1). Statistical methods including intraclass correlation showed a high agreement between Der p 1 mass fractions irrespective of the extracting medium. Our results suggest that all three buffers are suitable for the extraction of mite allergens and routine Der p 1 analysis in dust.
authors.affiliation: Institute for Medical Research and Occupational Health, Zagreb, Croatia. prester@imi.hr
authors.forename: Ljerka
authors.initials: L
authors.lastname: Prester
authors.forename: Jelena
authors.initials: J
authors.lastname: Kovačić
authors.forename: Jelena
authors.initials: J
authors.lastname: Macan
mesh_terms: D000485:Allergens / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000302:isolation & purification*; D060829:Arthropod Proteins / Q000302:isolation & purification*; D001512:Bedding and Linens; D001639:Bicarbonates / Q000737:chemistry; D001881:Borates / Q000737:chemistry; D002021:Buffers; D005591:Chemical Fractionation / Q000379:methods; D003546:Cysteine Endopeptidases / Q000302:isolation & purification*; D004391:Dust / Q000032:analysis*; D004781:Environmental Exposure / Q000032:analysis; D004784:Environmental Monitoring / Q000379:methods*; D006801:Humans; D007124:Immunoenzyme Techniques; D008925:Mites / Q000276:immunology*; D010710:Phosphates / Q000737:chemistry; D011863:Radioimmunoassay / Q000379:methods
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D001639:Bicarbonates; D001881:Borates; D002021:Buffers; D004391:Dust; D010710:Phosphates; C027043:ammonium bicarbonate; D003546:Cysteine Endopeptidases; C451162:Dermatophagoides pteronyssinus antigen p 1