title: The transcriptional control machinery as well as the cell wall integrity and its regulation are involved in the detoxification of the organic solvent dimethyl sulfoxide in Saccharomyces cerevisiae.
abstract: In the present study, we have identified 339 dimethyl sulfoxide (DMSO)-sensitive and nine DMSO-tolerant gene mutations in Saccharomyces cerevisiae through a functional genomics approach. Twelve of these identified DMSO-sensitive mutations are of genes involved in the general control of gene expression mediated by the SWR1 complex and the RNA polymerase II mediator complex, whereas 71 of them are of genes involved in the protein trafficking and vacuolar sorting processes. In addition, twelve of these DMSO-sensitive mutations are of genes involved in the cell wall integrity (CWI) and its regulation. DMSO-tolerant mutations are of genes mainly involved in the metabolism and the gene expression control. Therefore, the transcriptional control machinery, the CWI and its regulation as well as the protein trafficking and sorting process play critical roles in the DMSO detoxification in yeast cells.
authors.affiliation: Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China.
authors.forename: Lilin
authors.initials: L
authors.lastname: Zhang
authors.forename: Ningning
authors.initials: N
authors.lastname: Liu
authors.forename: Xiao
authors.initials: X
authors.lastname: Ma
authors.forename: Linghuo
authors.initials: L
authors.lastname: Jiang
mesh_terms: D001711:Biotransformation; D002473:Cell Wall / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000378:metabolism* / Q000633:toxicity*; D015966:Gene Expression Regulation, Fungal; D053858:Metabolic Networks and Pathways / Q000235:genetics; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D012997:Solvents / Q000378:metabolism* / Q000633:toxicity*; D014158:Transcription, Genetic
chemical_list: D012997:Solvents; D004121:Dimethyl Sulfoxide