title: Expression of pancreatic regenerating gene in lung and intestinal tissue             correlates with the severity of disease in rats with acute necrotizing pancreatitis.
abstract: The aim of this study was to investigate the expression pattern of regenerating gene I (Reg I) in the lung and intestinal tissues of rats with acute necrotizing pancreatitis (ANP), as well as the correlation of Reg I expression with lung and intestinal injury. Sprague-Dawley rats were randomly allocated to control (n=40) and ANP (n=80) groups. The rats in the control group received laparotomy only. In the ANP group, 3% sodium taurocholate was injected into the pancreatic duct to develop the ANP model. Reverse transcription-polymerase chain reaction (RT-PCR) was performed to detect the Reg I mRNA levels in the pancreas, intestine and lung. The pathological changes in the pancreas, intestine and lung were observed and the serum amylase levels, the wet/dry weight ratio of the lung and the permeability of the intestinal mucosa were measured. The measured parameters were found to correlate with the Reg I mRNA levels. Reg I mRNA was more highly expressed in the pancreas, intestine and lung in the ANP rats than in the control group. The Reg I expression levels were positively correlated with the pathological scores, serum amylase levels, lung pathological scores, lung tissue wet/dry ratios, intestinal pathological scores and intestinal permeability. The levels of Reg I were increased in the lung and intestinal tissue of the ANP rats and the expression levels of Reg I correlated closely with the severity of the lung and intestinal injury.
authors.affiliation: Department of General Surgery, Zhongda Hospital, Southeast University,                     Nanjing, Jiangsu 210009, P.R. China.
authors.forename: Hao-Lin
authors.initials: HL
authors.lastname: Hu
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Kong
authors.forename: Xin
authors.initials: X
authors.lastname: Shi
mesh_terms: D000681:Amylases / Q000097:blood; D000818:Animals; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000473:pathology; D051847:Lithostathine / Q000235:genetics / Q000378:metabolism*; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008297:Male; D010179:Pancreas / Q000378:metabolism / Q000473:pathology; D019283:Pancreatitis, Acute Necrotizing / Q000378:metabolism* / Q000473:pathology; D010539:Permeability; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012720:Severity of Illness Index
chemical_list: D051847:Lithostathine; D012333:RNA, Messenger; C497616:Reg1a protein, rat; D000681:Amylases