title: Production of L. pneumophila monospecies biofilms in a low-nutrient-concentration medium.
abstract: In aquatic environments such as water distribution systems, Legionella pneumophila persistence is -correlated to the presence of a biofilm. The method described here permits the formation of a monospecies L. pneumophila biofilm in microplates, enabling the screening of multiple parameters. The culture medium used has a low nutrient concentration compared to the classical Buffered Yeast Extract culture medium. Hence, bacterial growth occurs in the sessile phase, allowing the formation of three-dimensional structures.
authors.affiliation: Faculté de Pharmacie, Laboratoire de Génie Chimique, BioSym Department, Université de Toulouse, Toulouse, France. sophie@pecastaings.net
authors.forename: Sophie
authors.initials: S
authors.lastname: Pécastaings
authors.forename: Christine
authors.initials: C
authors.lastname: Roques
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D003470:Culture Media; D016952:Legionella pneumophila / Q000502:physiology*
chemical_list: D003470:Culture Media