title: Optimized technique for subretinal injections in mice.
abstract: Subretinal injections in mice become increasingly important. Currently, the most prominent application is in gene therapy of inherited eye diseases by means of viral vector delivery to photoreceptors or the retinal pigment epithelium (RPE). Since there are no large animal models for most of these diseases, genetically modified mouse models are commonly used in preclinical proof-of-concept studies. However, because of the relatively small mouse eye, adverse effects of the subretinal delivery procedure itself may interfere with the therapeutic outcome. The protocol described here concerns a transscleral pars plana subretinal injection in small eyes, and may be used for but not limited to virus-mediated gene transfer.
authors.affiliation: Division of Ocular Neurodegeneration, Centre for Ophthalmology, Institute for Ophthalmic Research, University of Tuebingen, Tuebingen, Germany. regine.muehlfriedel@med.uni-tuebingen.de
authors.forename: Regine
authors.initials: R
authors.lastname: Mühlfriedel
authors.forename: Stylianos
authors.initials: S
authors.lastname: Michalakis
authors.forename: Marina
authors.initials: M
authors.lastname: Garcia Garrido
authors.forename: Martin
authors.initials: M
authors.lastname: Biel
authors.forename: Mathias W
authors.initials: MW
authors.lastname: Seeliger
mesh_terms: D000758:Anesthesia / Q000379:methods; D000818:Animals; D000229:Dependovirus / Q000235:genetics* / Q000378:metabolism; D004867:Equipment Design; D018014:Gene Transfer Techniques / Q000295:instrumentation; D005822:Genetic Vectors / Q000008:administration & dosage* / Q000235:genetics; D056965:Injections, Intraocular / Q000295:instrumentation / Q000379:methods*; D051379:Mice; D012160:Retina / Q000378:metabolism*