title: Evaluation of bleeding on probing and gingival crevicular fluid enzyme activity for detection of periodontally active sites during supportive periodontal therapy.
abstract: This study aimed to analyze the enzyme activity in gingival crevicular fluid (GCF) and its association with clinical parameters, especially bleeding on probing (BOP), and thus reconsider the significance and accuracy of recording BOP. A total of 184 patients who had entered supportive periodontal therapy were selected and GCF was collected from 401 sites before recording the clinical parameters, probing pocket depth (PPD), BOP, clinical attachment level, gingival index and plaque index. The enzyme activity of neutrophil elastase and aspartate aminotransferase and amount of protein in GCF were also analyzed. In the clinical parameters for biochemical data, amount of GCF showed the most correlation. A cut-off value for BOP and PPD were determined by the ROC curve and Youden index. Analysis was performed with all clinical parameters and biochemical data. Of the 401 sites, 51 were less than the cut-off value and were BOP-negative. On the other hand, 29 sites had values more than the cut-off value, with 14 BOP-negative sites and 15 BOP-positive sites. A conclusion is as follows: twenty-nine sites with values more than the cut-off value were diagnosed as sites requiring periodontal management, however, 14 of these were BOP-negative. These results suggest that combining other biochemical tests with examination of BOP and PPD may improve the validity of periodontal disease diagnosis. In future studies, it will be essential to find a marker that can precisely detect periodontal disease activity, and to develop a diagnostic tool for chair-side use.
authors.affiliation: Department of Periodontology, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-8159, Japan, hiroshi@tky.ndu.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ito
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Numabe
authors.forename: Satoshi
authors.initials: S
authors.lastname: Sekino
authors.forename: Etsuko
authors.initials: E
authors.lastname: Murakashi
authors.forename: Hitomi
authors.initials: H
authors.lastname: Iguchi
authors.forename: Shuichi
authors.initials: S
authors.lastname: Hashimoto
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sasaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Yaegashi
authors.forename: Kazushi
authors.initials: K
authors.lastname: Kunimatsu
authors.forename: Hideki
authors.initials: H
authors.lastname: Takai
authors.forename: Masaru
authors.initials: M
authors.lastname: Mezawa
authors.forename: Yorimasa
authors.initials: Y
authors.lastname: Ogata
authors.forename: Hisashi
authors.initials: H
authors.lastname: Watanabe
authors.forename: Satsuki
authors.initials: S
authors.lastname: Hagiwara
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Izumi
authors.forename: Yuka
authors.initials: Y
authors.lastname: Hiroshima
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Kido
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Nagata
mesh_terms: D000368:Aged; D005260:Female; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D005884:Gingival Hemorrhage / Q000209:etiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010514:Periodontal Pocket; D010518:Periodontitis / Q000150:complications / Q000175:diagnosis* / Q000628:therapy