title: Isolation and characterization of human hair follicle epithelial cells.
abstract: The mammalian hair follicle epithelial component contains various lineages of keratinocytes as well as their progenitor/stem cells. To characterize the subpopulations contained within this component and assess their functional capacity, the development of a feasible method to isolate them is greatly needed. In this chapter we describe a standard method by which a small subset of human follicular keratinocytes can be isolated for subsequent analysis. Detailed methods for enzymatic digestion of fresh human scalp, flow cytometry, cultivation of sorted cells and a colony forming assay are described.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shizuoka Cancer Center, Shizuoka, Japan.
authors.forename: Keita
authors.initials: K
authors.lastname: Inoue
authors.forename: Kotaro
authors.initials: K
authors.lastname: Yoshimura
mesh_terms: D000906:Antibodies / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002453:Cell Cycle; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D018859:Hair Follicle / Q000166:cytology* / Q000601:surgery; D006801:Humans; D015603:Keratinocytes / Q000166:cytology; D042282:Microdissection; D059010:Single-Cell Analysis; D012985:Software Design; D013194:Staining and Labeling
chemical_list: D000906:Antibodies; D015415:Biomarkers