title: Plasmid IL-12 electroporation in melanoma.
abstract: Intratumoral gene electroporation uses electric charges to facilitate entry of plasmid DNA into cells in a reproducible and highly efficient manner, especially to accessible sites such as cutaneous and subcutaneous melanomas. Effective for locally treated disease, electroporation of plasmid DNA encoding interleukin-12 can also induce responses in untreated distant disease, suggesting that adaptive immune responses are being elicited that can target melanoma-associated antigens. In vivo electroporation with immunomodulatory cytokine DNA is a promising approach that can trigger systemic anti-tumor immune responses without the systemic toxicity associated with intravenous cytokine delivery and potentially offer complete long-term tumor regression.
authors.affiliation: Department of Medicine; University of California-San Francisco, CA, USA. Edward.cha@ucsf.edu
authors.forename: Edward
authors.initials: E
authors.lastname: Cha
authors.forename: Adil
authors.initials: A
authors.lastname: Daud
mesh_terms: D056704:Adaptive Immunity / Q000276:immunology; D000818:Animals; D016207:Cytokines / Q000235:genetics; D018274:Electroporation / Q000379:methods*; D006801:Humans; D018664:Interleukin-12 / Q000235:genetics*; D008545:Melanoma / Q000628:therapy*
chemical_list: D016207:Cytokines; D018664:Interleukin-12