title: Analysis of collagen fiber domain organization by Fourier second harmonic generation microscopy.
abstract: We present an automated and systematic two-dimensional discrete Fourier transform (2D-FFT) approach to analyze collagen fiber organization through the use of second harmonic generation (SHG) microscopy. Average orientations of individual domains and Ising-like order parameters introduced to characterize the correlation between orientations of adjacent domains may be used to quantitatively characterize fibrous tissues. Our approach was applied to analyze tissues including rat tail tendon, mouse skin, bovine corneas, and human corneas. We also show that collagen fiber organization in normal and keratokonus human corneas may be distinguished. The current approach may be used for the quantitative differentiation of SHG collagen fiber morphology in different tissues and may be applied for diagnostic purposes.
authors.affiliation: National Taiwan University, Department of Physics, Taipei 106, Taiwan.
authors.forename: Ara
authors.initials: A
authors.lastname: Ghazaryan
authors.forename: Halley F
authors.initials: HF
authors.lastname: Tsai
authors.forename: Gor
authors.initials: G
authors.lastname: Hayrapetyan
authors.forename: Wei-Liang
authors.initials: WL
authors.lastname: Chen
authors.forename: Yang-Fang
authors.initials: YF
authors.lastname: Chen
authors.forename: Myung Yung
authors.initials: MY
authors.lastname: Jeong
authors.forename: Chang-Seok
authors.initials: CS
authors.lastname: Kim
authors.forename: Shean-Jen
authors.initials: SJ
authors.lastname: Chen
authors.forename: Chen-Yuan
authors.initials: CY
authors.lastname: Dong
mesh_terms: D000818:Animals; D002417:Cattle; D003315:Cornea / Q000737:chemistry; D024022:Fibrillar Collagens / Q000737:chemistry* / Q000648:ultrastructure*; D005583:Fourier Analysis; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D051379:Mice; D008853:Microscopy / Q000379:methods*; D010363:Pattern Recognition, Automated; D051381:Rats; D012867:Skin / Q000737:chemistry; D013710:Tendons / Q000737:chemistry
chemical_list: D024022:Fibrillar Collagens