title: Efficient multi-site-directed mutagenesis directly from genomic template.
abstract: In this article, the traditional multi-site-directed mutagenesis method based on overlap extension  PCR was improved specifically for complicated templates, such as genomic sequence or complementary DNA. This method was effectively applied for multi-site-directed mutagenesis directly from mouse genomic DNA, as well as for combination, deletion or insertion of DNA fragments.
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Center of Bone Metabolism and Repair, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042 China.
authors.forename: Fengtao
authors.initials: F
authors.lastname: Luo
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Du
authors.forename: Tujun
authors.initials: T
authors.lastname: Weng
authors.forename: Xuan
authors.initials: X
authors.lastname: Wen
authors.forename: Junlan
authors.initials: J
authors.lastname: Huang
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D023281:Genomics; D051379:Mice; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction; D013698:Templates, Genetic
chemical_list: D017931:DNA Primers; D004247:DNA