title: Genomic characterization of a newly discovered coronavirus associated with acute respiratory distress syndrome in humans.
abstract: A novel human coronavirus (HCoV-EMC/2012) was isolated from a man with acute pneumonia and renal failure in June 2012. This report describes the complete genome sequence, genome organization, and expression strategy of HCoV-EMC/2012 and its relation with known coronaviruses. The genome contains 30,119 nucleotides and contains at least 10 predicted open reading frames, 9 of which are predicted to be expressed from a nested set of seven subgenomic mRNAs. Phylogenetic analysis of the replicase gene of coronaviruses with completely sequenced genomes showed that HCoV-EMC/2012 is most closely related to Tylonycteris bat coronavirus HKU4 (BtCoV-HKU4) and Pipistrellus bat coronavirus HKU5 (BtCoV-HKU5), which prototype two species in lineage C of the genus Betacoronavirus. In accordance with the guidelines of the International Committee on Taxonomy of Viruses, and in view of the 75% and 77% amino acid sequence identity in 7 conserved replicase domains with BtCoV-HKU4 and BtCoV-HKU5, respectively, we propose that HCoV-EMC/2012 prototypes a novel species in the genus Betacoronavirus. HCoV-EMC/2012 may be most closely related to a coronavirus detected in Pipistrellus pipistrellus in The Netherlands, but because only a short sequence from the most conserved part of the RNA-dependent RNA polymerase-encoding region of the genome was reported for this bat virus, its genetic distance from HCoV-EMC remains uncertain. HCoV-EMC/2012 is the sixth coronavirus known to infect humans and the first human virus within betacoronavirus lineage C.
  
  
  OBJECTIVE
  Coronaviruses are capable of infecting humans and many animal species. Most infections caused by human coronaviruses are relatively mild. However, the outbreak of severe acute respiratory syndrome (SARS) caused by SARS-CoV in 2002 to 2003 and the fatal infection of a human by HCoV-EMC/2012 in 2012 show that coronaviruses are able to cause severe, sometimes fatal disease in humans. We have determined the complete genome of HCoV-EMC/2012 using an unbiased virus discovery approach involving next-generation sequencing techniques, which enabled subsequent state-of-the-art bioinformatics, phylogenetics, and taxonomic analyses. By establishing its complete genome sequence, HCoV-EMC/2012 was characterized as a new genotype which is closely related to bat coronaviruses that are distant from SARS-CoV. We expect that this information will be vital to rapid advancement of both clinical and vital research on this emerging pathogen.
authors.affiliation: Viroscience Lab, Erasmus MC, Rotterdam, The Netherlands.
authors.forename: Sander
authors.initials: S
authors.lastname: van Boheemen
authors.forename: Miranda
authors.initials: M
authors.lastname: de Graaf
authors.forename: Chris
authors.initials: C
authors.lastname: Lauber
authors.forename: Theo M
authors.initials: TM
authors.lastname: Bestebroer
authors.forename: V Stalin
authors.initials: VS
authors.lastname: Raj
authors.forename: Ali Moh
authors.initials: AM
authors.lastname: Zaki
authors.forename: Albert D M E
authors.initials: AD
authors.lastname: Osterhaus
authors.forename: Bart L
authors.initials: BL
authors.lastname: Haagmans
authors.forename: Alexander E
authors.initials: AE
authors.lastname: Gorbalenya
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Snijder
authors.forename: Ron A M
authors.initials: RA
authors.lastname: Fouchier
mesh_terms: D016000:Cluster Analysis; D017934:Coronavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D009426:Netherlands; D016366:Open Reading Frames; D010802:Phylogeny; D011024:Pneumonia, Viral / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics*; D012128:Respiratory Distress Syndrome / Q000821:virology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D012367:RNA, Viral