title: Novel application of Mahua (Madhuca sp.) flowers for augmented protease production from Aeromonas sp. S1.
abstract: The present study explored the utilization of Mahua (Madhuca sp.) flowers, a major non-timber forest product (NTFP) of India, as a low-cost, natural substrate for protease production under submerged fermentation. Bacterial strain Aeromonas sp. Si1, previously reported by us, was used as the protease producer. Using Mahua flower extract (MFE) as the medium additive, the protease production could successfully be enhanced by 5.6-fold (564.5 UmL-1) after 24 h of fermentation under optimized conditions compared with initial production of 99.9 UmL' in the absence of MFE. The cultural parameters for optimum production of protease were determined to be: incubation time-24 h; pH-7.0; MFE concentration-5% (v/v); inoculum size-0.3% (v/v) and agitation rate-200 rpm. The results obtained demonstrate the potential of cheaper and abundantly available Mahua flowers for induction of proteases, and thus offer a new approach for value addition to this biomass through industrial enzyme production.
authors.affiliation: University School of Environment Management, Guru Gobind Singh Indraprastha University, Sector 16-C, Dwarka, New Delhi 10078, India.
authors.forename: Amrik
authors.initials: A
authors.lastname: Bhattacharya
authors.forename: Vandana
authors.initials: V
authors.lastname: Saini
authors.forename: Anshu
authors.initials: A
authors.lastname: Gupta
mesh_terms: D000333:Aeromonas / Q000201:enzymology* / Q000254:growth & development*; D001431:Bacteriological Techniques; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D003470:Culture Media; D005285:Fermentation; D035264:Flowers / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D032128:Madhuca / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry
chemical_list: D003470:Culture Media; D010936:Plant Extracts; D010447:Peptide Hydrolases