title: [MicroRNA profiling in T cells of peripheral blood mononuclear cell from patients with primary biliary cirrhosis].
abstract: OBJECTIVE
  To explore the expression pattern of microRNA (miRNA) in T cells of peripheral blood mononuclear cell (PBMC) from patients with primary biliary cirrhosis (PBC).
  
  
  METHODS
  The expression profile of miRNA in T cells of PBMC was determined by microarray assay and validated by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
  
  
  RESULTS
  In comparison with the healthy controls, 23 miRNA were down-regulated and 2 miRNA had a higher expression (all P < 0.05). As revealed by qRT-PCR, the expressions of miR-346, miR-17-5p, miR-20a and miR-let-7b decreased obviously while miR-451 and miR-129 became up-regulated. The results were in agreement with those of microarray.
  
  
  CONCLUSIONS
  The PBC patients and healthy controls have significantly different expression profiles of microRNA in T cells of PBMC. The differential expression of microRNA may be involved in the pathogenesis of PBC.
authors.affiliation: Department of Laboratory Medicine, No.100 Hospital of PLA, Suzhou, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Qian
authors.forename: Huai-zhou
authors.initials: HZ
authors.lastname: Wang
authors.forename: Hua-jie
authors.initials: HJ
authors.lastname: Fan
authors.forename: Ming-li
authors.initials: ML
authors.lastname: Gu
authors.forename: Chuan-lu
authors.initials: CL
authors.lastname: Ren
authors.forename: An-mei
authors.initials: AM
authors.lastname: Deng
authors.forename: Ren-qian
authors.initials: RQ
authors.lastname: Zhong
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008105:Liver Cirrhosis, Biliary / Q000235:genetics* / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D035683:MicroRNAs