title: Analyzing caspase-1 activation during Legionella pneumophila infection in macrophages.
abstract: Caspase-1 is a critical factor in the innate immune response to Legionella pneumophila. The development of methods for analyzing caspase-1 activation pathways and downstream caspase-1-associated activities has helped in understanding the regulation of this protease and the signaling components involved. Here we outline methods for directly detecting active caspase-1, measuring caspase-1 activities and analyzing components involved in the regulation of caspase-1 during L. pneumophila infection in macrophages.
authors.affiliation: Section of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, CT, USA.
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Case
authors.forename: Craig R
authors.initials: CR
authors.lastname: Roy
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001854:Bone Marrow Cells / Q000201:enzymology / Q000382:microbiology; D053468:CARD Signaling Adaptor Proteins; D002135:Calcium-Binding Proteins / Q000378:metabolism; D020170:Caspase 1 / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004789:Enzyme Activation; D016952:Legionella pneumophila / Q000254:growth & development / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism* / Q000382:microbiology*; D051379:Mice; D051616:Neuronal Apoptosis-Inhibitory Protein / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; D053468:CARD Signaling Adaptor Proteins; D002135:Calcium-Binding Proteins; D003598:Cytoskeletal Proteins; C512299:Ipaf protein, mouse; C496806:Naip5 protein, mouse; D051616:Neuronal Apoptosis-Inhibitory Protein; C498534:Pycard protein, mouse; D020170:Caspase 1