title: The effects of graphene oxide nanosheets localized on F-actin filaments on cell-cycle alterations.
abstract: Graphene oxide (GO) is considered to be a promising nanomaterial for biomedical applications due to its small two-dimensional shape besides its electrical and mechanical properties. However, only a few data concerning the cell responses to this material have been described and the GO biocompatibility has not been yet fully assessed. In the present study, graphene oxide nanosheets (GOs) decorated with 1-arm (1-GOs) and 6-arm (6-GOs) poly(ethylene glycol-amine) (PEG) have been incubated with cultured Saos-2 osteoblasts, MC3T3-E1 preosteoblasts and RAW-264.7 macrophages to analyze several key cell markers for in vitro biocompatibility evaluation. The results demonstrate that, after internalization, GO nanosheets are localized on F-actin filaments inducing cell-cycle alterations, apoptosis and oxidative stress in these cell types. The observed GOs effects must be considered in further studies focused on photothermal cancer therapy as a synergistic factor.
authors.affiliation: Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense, 28040 Madrid, Spain.
authors.forename: María-Concepción
authors.initials: MC
authors.lastname: Matesanz
authors.forename: Mercedes
authors.initials: M
authors.lastname: Vila
authors.forename: María-José
authors.initials: MJ
authors.lastname: Feito
authors.forename: Javier
authors.initials: J
authors.lastname: Linares
authors.forename: Gil
authors.initials: G
authors.lastname: Gonçalves
authors.forename: María
authors.initials: M
authors.lastname: Vallet-Regi
authors.forename: Paula-Alexandrina A P
authors.initials: PA
authors.lastname: Marques
authors.forename: María-Teresa
authors.initials: MT
authors.lastname: Portolés
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure; D000199:Actins / Q000378:metabolism* / Q000648:ultrastructure; D002453:Cell Cycle / Q000187:drug effects* / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D006108:Graphite / Q000494:pharmacology*; D053758:Nanoparticles / Q000008:administration & dosage*; D010087:Oxides / Q000494:pharmacology
chemical_list: D000199:Actins; D018797:Cell Cycle Proteins; D010087:Oxides; D006108:Graphite