title: [Design and application of high-throughput screening tools: a review].
abstract: As an efficient and promising protein engineering strategy, directed evolution includes the construction of mutant libraries and screening of desirable mutants. A rapid and high-throughput screening method has played a critical role in the successful application of directed evolution strategy. We reviewed several high-throughput screening tools which have great potential to be applied in directed evolution. The development of powerful high-throughput screening tools will make great contributions to the advancement of protein engineering.
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. tangsy@im.ac.cn
authors.forename: Shuangyan
authors.initials: S
authors.lastname: Tang
authors.forename: Chaoning
authors.initials: C
authors.lastname: Liang
authors.forename: Peixia
authors.initials: P
authors.lastname: Jiang
mesh_terms: D019020:Directed Molecular Evolution / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods*; D016297:Mutagenesis, Site-Directed / Q000379:methods; D050505:Mutant Proteins / Q000235:genetics; D015202:Protein Engineering / Q000379:methods*
chemical_list: D050505:Mutant Proteins