title: Investigation of silicon-based nanostructure morphology and chemical termination on laser desorption ionization mass spectrometry performance.
abstract: We have evaluated the laser desorption ionization mass spectrometry (LDI-MS) performance of six nanostructured silicon surfaces of different morphologies and chemical functionalizations. The substrates have been synthesized either by metal-assisted etching method or by vapor-liquid-solid (VLS) growth technique. In addition to the commercial nanostructured silicon-based surface (NALDI) target plates, serving as reference, the homemade surfaces have been evaluated in mass spectrometry experiments conducted with peptide solutions mimicking tryptic digests. LDI surfaces synthesized by metal-assisted etching method were the most efficient in terms of signal intensities and number of detected peptides. The surface providing the best LDI-MS performance was composed of two nanostructured layers. Interestingly, we also observed a significant influence of the type of organic coating (hydrocarbon vs fluorocarbon) on peptide ionization discrimination.
authors.affiliation: Institut des Biomolécules Max Mousseron, UMR 5247, Universités Montpellier 1 et 2, CNRS, Bâtiment Chimie (17), Université Montpellier 2, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Dupré
authors.forename: Christine
authors.initials: C
authors.lastname: Enjalbal
authors.forename: Sonia
authors.initials: S
authors.lastname: Cantel
authors.forename: Jean
authors.initials: J
authors.lastname: Martinez
authors.forename: Nacéra
authors.initials: N
authors.lastname: Megouda
authors.forename: Toufik
authors.initials: T
authors.lastname: Hadjersi
authors.forename: Rabah
authors.initials: R
authors.lastname: Boukherroub
authors.forename: Yannick
authors.initials: Y
authors.lastname: Coffinier
mesh_terms: D049329:Nanostructures / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D012825:Silicon