title: Combining genome shuffling and interspecific hybridization among Streptomyces improved ε-poly-L-lysine production.
abstract: Here we first improved the ε-PL productivity in five species of wild-type strains in Streptomyces (Streptomyces padanus, Streptomyces griseofuscus, Streptomyces graminearus, Streptomyces hygroscopicus, and Streptomyces albulus) by genome shuffling. Then all the shuffled strains were suffered from an interspecific hybridization through stochastic protoplast fusion. One hybrid designated FEEL-1 was selected by morphology and spore color with ε-PL production of 1.12 g/L in shake flask, about 2.75-fold higher than that in wild types. The ε-PL production of FEEL-1 was then obtained as 24.5 g/L in fed-batch fermentation, which was 63-81 % higher than those in shuffled strains. Random amplified polymorphic DNA revealed that FEEL-1 was probably hybridized from S. padanus, S. griseofuscus, and S. albulus. Activities of several enzymes in FEEL-1 (hexokinase, phosphoenolpyruvate carboxylase, aspartokinase, and citrate synthase) were more active than those in shuffled strains, which was a possible reason for the enhancement of ε-PL production. This research highlights the importance of genome shuffling along with interspecific hybridization as a new breeding strategy for improving phenotype of industrial strains.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, People's Republic of China.
authors.forename: Shu
authors.initials: S
authors.lastname: Li
authors.forename: Xusheng
authors.initials: X
authors.lastname: Chen
authors.forename: Chuanliang
authors.initials: C
authors.lastname: Dong
authors.forename: Fulin
authors.initials: F
authors.lastname: Zhao
authors.forename: Lei
authors.initials: L
authors.lastname: Tang
authors.forename: Zhonggui
authors.initials: Z
authors.lastname: Mao
mesh_terms: D032621:DNA Shuffling; D005285:Fermentation; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D016680:Genome, Bacterial; D006824:Hybridization, Genetic; D011107:Polylysine / Q000096:biosynthesis*; D013302:Streptomyces / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D011107:Polylysine