title: Assessment of fetomaternal hemorrhage by Kleihauer-Betke test, flow cytometry and α-fetoprotein after invasive obstetric procedures.
abstract: OBJECTIVE
  The aim of this study was to evaluate the passage of fetal red blood cells to the maternal circulation, after invasive obstetric procedures, through the Kleihauer-Betke test, flow cytometry and by measurement of maternal serum alpha-fetoprotein level.
  
  
  METHODS
  This prospective descriptive study with patients submitted to amniocentesis, cordocentesis, chorionic villus sampling (CVS), amnioreduction and ventriculoamniotic shunt was performed for karyotype analysis, treatment of hydrocephalus and polyhydramnios and to assess fetal lung maturity. Maternal blood samples were collected before and 60 minutes after the invasive obstetric procedure to search for fetal erythrocytes using the Kleihauer-Betke test, flow cytometry and serum alpha-fetoprotein measurement.
  
  
  RESULTS
  Ten invasive obstetric procedures were performed. The mean age of the patients was 29.2 years and the mean gestational age was 29.6 weeks. The procedures were: five amniocenteses, two cordocenteses, one CVS, one ventriculo-amniotic shunt and one amnioreduction with cephalocentesis. The indications for the procedures were: karyotype analysis in five patients, fetal lung maturity assessment in two patients, amnioreduction in one patient, fetal hydrocephalus shunt in one patient and polyhydramnios related to hydranencephaly in one patient. Regarding the path of puncture, three procedures were accomplished through the placenta and seven apart from it. All punctures were successful at the first attempt. There was no significant increase of fetal erythrocyte quantity in maternal blood samples using the Kleihauer-Betke test. After cordocentesis, a significant increase of fetal erythrocytes was detected by flow cytometry and serum alpha-fetoprotein measurement.
  
  
  CONCLUSIONS
  Invasive obstetric procedures during prenatal care are safe when performed by experienced professionals using adequate techniques, with minimal chance of passage of fetal erythrocytes from the fetal compartment.
authors.affiliation: Department of Obstetrics, Federal University of São Paulo (UNIFESP), São Paulo, SP, Brazil.
authors.forename: D
authors.initials: D
authors.lastname: Meleti
authors.forename: A C R
authors.initials: AC
authors.lastname: Caetano
authors.forename: T
authors.initials: T
authors.lastname: Boute
authors.forename: L G
authors.initials: LG
authors.lastname: de Oliveira
authors.forename: E
authors.initials: E
authors.lastname: Araujo Júnior
authors.forename: L M M
authors.initials: LM
authors.lastname: Nardozza
authors.forename: A F
authors.initials: AF
authors.lastname: Moron
mesh_terms: D000328:Adult; D000649:Amniocentesis; D015193:Chorionic Villi Sampling; D017218:Cordocentesis; D004912:Erythrocytes; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D005315:Fetal Diseases / Q000601:surgery; D005319:Fetal Hemoglobin / Q000032:analysis; D005331:Fetomaternal Transfusion / Q000175:diagnosis* / Q000209:etiology; D005434:Flow Cytometry; D006801:Humans; D006849:Hydrocephalus / Q000601:surgery; D007621:Karyotyping; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000009:adverse effects*; D011446:Prospective Studies; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D000509:alpha-Fetoproteins; D005319:Fetal Hemoglobin