title: Development and validation of automatic HS-SPME with a gas chromatography-ion trap/mass spectrometry method for analysis of volatiles in wines.
abstract: An automated headspace solid-phase microextraction (HS-SPME) combined with gas chromatography-ion trap/mass spectrometry (GC-IT/MS) was developed in order to quantify a large number of volatile compounds in wines such as alcohols, ester, norisoprenoids and terpenes. The procedures were optimized for SPME fiber selection, pre-incubation temperature and time, extraction temperature and time, and salt addition. A central composite experimental design was used in the optimization of the extraction conditions. The volatile compounds showed optimal extraction using a DVB/CAR/PDMS fiber, incubation of 5 ml of wine with 2g NaCl at 45 °C during 5 min, and subsequent extraction of 30 min at the same temperature. The method allowed the identification of 64 volatile compounds. Afterwards, the method was validated successfully for the most significant compounds and was applied to study the volatile composition of different white wines.
authors.affiliation: Instituto de Química, Universidade Federal do Rio de Janeiro, Avenida Athos da Silveira Ramos, 149, Bloco A, Laboratório 626 A, 21941-909, Cidade Universitária, Rio de Janeiro, Brazil. elisabete@eq.ufrj.br
authors.forename: Elisabete
authors.initials: E
authors.lastname: Paula Barros
authors.forename: Nathalie
authors.initials: N
authors.lastname: Moreira
authors.forename: Giuliano
authors.initials: G
authors.lastname: Elias Pereira
authors.forename: Selma Gomes Ferreira
authors.initials: SG
authors.lastname: Leite
authors.forename: Claudia
authors.initials: C
authors.lastname: Moraes Rezende
authors.forename: Paula
authors.initials: P
authors.lastname: Guedes de Pinho
mesh_terms: D001331:Automation; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D057230:Limit of Detection; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction / Q000379:methods*; D055549:Volatile Organic Compounds / Q000032:analysis*; D014920:Wine / Q000032:analysis*
chemical_list: D055549:Volatile Organic Compounds