title: [Quantity of Desulfovibrios and analysis of intestinal microbiota diversity in health and intestinal disease people in Wuxi, Jiangsu province].
abstract: OBJECTIVE
  This paper provides an overview of Desulfovibrio (DSV) incidence and its effect on bacterial diversity in human gastrointestinal tract of four groups: ulcerative colitis (UC), colorectal cancer (CRC), polypus (PP) and the healthy control (H).
  
  
  METHODS
  Real time fluorescence quantitative PCR (RT-PCR) assays were used to enumerate DSV in gastrointestinal tract of 58 subjects. Diversity of gut microbiota was analyzed by PCR-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) and 16S rRNA V3 sequencing.
  
  
  RESULTS
  RT-PCR detected DSV in all samples. Significantly increased numbers of DSV were observed for UC and PP groups compared with CRC and H groups. No significant difference was observed for CRC and H groups with gene copy numbers of DSV. Alterations of DSV and gut microbiota were observed in disease groups.
  
  
  CONCLUSIONS
  We found that quantity and diversity of DSV are significantly increased in UC and PP compared to controls. The increased numbers of DSV in disease groups suggests a possible harmful role.
authors.affiliation: Research Center of Food Biotechnology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. junrong888@126.com
authors.forename: Junrong
authors.initials: J
authors.lastname: Ding
authors.forename: Qiuxiang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Liu
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.forename: Fengwei
authors.initials: F
authors.lastname: Tian
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003901:Desulfovibrio / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007410:Intestinal Diseases / Q000382:microbiology*; D007422:Intestines / Q000382:microbiology*; D008297:Male; D054892:Metagenome; D008875:Middle Aged; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S