title: [Development of biochip for determination of Y-haplogroups occuring in Russian populations].
abstract: Biochip has been developed which allowed to determine the following Y-chromosome haplogroups: C, DE, G, H, I, J, L, N, O, R in a DNA sample. The following SNPs were choosen as haplogroup markers: M130, M145, P257, M69, U179, M304, M185, M231, M175, P224, correspondingly. The genotyping included two-round PCR with fluorescent label incorporation into PCR product followed by hybridization with immobilized probes on biochip. The analysis of fluorescent signal ratios in pairs of immobilized probes "wild-type probe"--"group specific probe" for each of choosen polymorphic markers showed high accuracy of Y-haplogroup genotyping using biochip. The reliability of genotyping was confirmed by direct sequencing.
authors.forename: D O
authors.initials: DO
authors.lastname: Fesenko
authors.forename: O V
authors.initials: OV
authors.lastname: Kalennik
authors.forename: V E
authors.initials: VE
authors.lastname: Barskiĭ
authors.forename: A S
authors.initials: AS
authors.lastname: Zasedatelev
authors.forename: T V
authors.initials: TV
authors.lastname: Nasedkina
mesh_terms: D041322:Chromosomes, Human, Y / Q000235:genetics*; D015342:DNA Probes; D005456:Fluorescent Dyes; D005819:Genetic Markers; D006239:Haplotypes; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results; D012426:Russia; D044465:White People / Q000235:genetics*
chemical_list: D015342:DNA Probes; D005456:Fluorescent Dyes; D005819:Genetic Markers