title: Complete genome sequence of the novel lytic avian pathogenic coliphage NJ01.
abstract: Bacteriophages of the C3 morphotype, characterized by very long heads that exceed their width several times, are extremely rare among the Podoviridae family members and constitute only 0.5% of over 5,500 phages that have been examined by the electron microscope (H. W. Ackermann, Arch. Virol. 152:227-243, 2007; H. W. Ackermann, Arch. Virol. 146:843-857, 2001). To date, among those phages proven to be C3, only coliphage phiEco32, Lactococcus phage KSY1, Vibrio phage 71A-6, and Salmonella enterica phage 7-11, but no avian pathogenic Escherichia coli (APEC) bacteriophages, have been completely sequenced (A. Chopin, H. Deveau, S. D. Ehrlich, S. Moineau, and M. C. Chopin, Virology 365:1-9, 2007; S. A. Khan, et al., Mol. Cell Probes 15:61-69, 2001; A. M. Kropinski, E. J. Lingohr, H. W. Ackermann, Arch. Virol. 156:149-151, 2011; D. Savalia, et al., J. Mol. Biol. 377:774-789, 2008) and are available in public databases. We isolated a bacteriophage from a scale duck market in Nanjing, Jiangsu province, named NJ01, that infects APEC. Sequence and morphological analyses revealed that phage NJ01 is a C3-like bacteriophage and belongs to the Podoviridae family. Here, we announce the complete genome sequence of phage NJ01 and submit the results of our analysis.
authors.affiliation: Key Lab Animal Bacteriology, Ministry of Agriculture, College of veterinary medicine, Nanjing Agricultural University, Nanjing, Jiang Su, People's Republic of China.
authors.forename: Yanzhe
authors.initials: Y
authors.lastname: Li
authors.forename: Mianmian
authors.initials: M
authors.lastname: Chen
authors.forename: Fang
authors.initials: F
authors.lastname: Tang
authors.forename: Huochun
authors.initials: H
authors.lastname: Yao
authors.forename: Chengping
authors.initials: C
authors.lastname: Lu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001717:Birds / Q000821:virology*; D003090:Coliphages / Q000235:genetics*; D016679:Genome, Viral; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D016366:Open Reading Frames