title: Human T-cells directed to seasonal influenza A virus cross-react with 2009 pandemic influenza A (H1N1) and swine-origin triple-reassortant H3N2 influenza viruses.
abstract: Virus-specific CD8(+) T-cells contribute to protective immunity against influenza A virus (IAV) infections. As the majority of these cells are directed to conserved viral proteins, they may afford protection against IAVs of various subtypes. The present study assessed the cross-reactivity of human CD8(+) T-lymphocytes, induced by infection with seasonal A (H1N1) or A (H3N2) influenza virus, with 2009 pandemic influenza A (H1N1) virus [A(H1N1)pdm09] and swine-origin triple-reassortant A (H3N2) [A(H3N2)v] viruses that are currently causing an increasing number of human cases in the USA. It was demonstrated that CD8(+) T-cells induced after seasonal IAV infections exerted lytic activity and produced gamma interferon upon in vitro restimulation with A(H1N1)pdm09 and A(H3N2)v influenza A viruses. Furthermore, CD8(+) T-cells directed to A(H1N1)pdm09 virus displayed a high degree of cross-reactivity with A(H3N2)v viruses. It was concluded that cross-reacting T-cells had the potential to afford protective immunity against A(H1N1)pdm09 viruses during the pandemic and offer some degree of protection against infection with A(H3N2)v viruses.
authors.affiliation: Department of Viroscience, Erasmus Medical Center, 3000 CA Rotterdam, The Netherlands.
authors.forename: Marine L B
authors.initials: MLB
authors.lastname: Hillaire
authors.affiliation: Department of Viroscience, Erasmus Medical Center, 3000 CA Rotterdam, The Netherlands.
authors.forename: Stella E
authors.initials: SE
authors.lastname: Vogelzang-van Trierum
authors.affiliation: Department of Viroscience, Erasmus Medical Center, 3000 CA Rotterdam, The Netherlands.
authors.forename: Joost H C M
authors.initials: JHCM
authors.lastname: Kreijtz
authors.affiliation: Department of Viroscience, Erasmus Medical Center, 3000 CA Rotterdam, The Netherlands.
authors.forename: Gerrie
authors.initials: G
authors.lastname: de Mutsert
authors.affiliation: Department of Viroscience, Erasmus Medical Center, 3000 CA Rotterdam, The Netherlands.
authors.forename: Ron A M
authors.initials: RAM
authors.lastname: Fouchier
authors.affiliation: Viroclinics Biosciences BV, 3015 GE Rotterdam, The Netherlands.|Department of Viroscience, Erasmus Medical Center, 3000 CA Rotterdam, The Netherlands.
authors.forename: Albert D M E
authors.initials: ADME
authors.lastname: Osterhaus
authors.affiliation: Viroclinics Biosciences BV, 3015 GE Rotterdam, The Netherlands.|Department of Viroscience, Erasmus Medical Center, 3000 CA Rotterdam, The Netherlands.
authors.forename: Guus F
authors.initials: GF
authors.lastname: Rimmelzwaan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000502:physiology*; D045744:Cell Line, Tumor; D000939:Epitopes; D014644:Genetic Variation; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D053122:Influenza A Virus, H3N2 Subtype / Q000276:immunology*; D009980:Influenza A virus / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology / Q000821:virology*; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D058873:Pandemics
chemical_list: D000939:Epitopes