title: Strategy for the creation of clinical grade hESC line banks that HLA-match a target population.
abstract: Here, we describe a pre-derivation embryo haplotyping strategy that we developed in order to maximize the efficiency and minimize the costs of establishing banks of clinical grade hESC lines in which human leukocyte antigen (HLA) haplotypes match a significant proportion of the population. Using whole genome amplification followed by medium resolution HLA typing using PCR amplification with sequence-specific primers (PCR-SSP), we have typed the parents, embryos and hESC lines from three families as well as our eight clinical grade hESC lines and shown that this technical approach is rapid, reliable and accurate. By employing this pre-derivation strategy where, based on HLA match, embryos are selected for a GMP route on day 3-4 of development, we would have drastically reduced our cGMP laboratory running costs.
authors.affiliation: Embryonic Stem Cell Laboratories, Guy's Assisted Conception Unit, Division of Women's Health, King's College School of Medicine, London, UK.
authors.forename: Laureen
authors.initials: L
authors.lastname: Jacquet
authors.forename: Emma
authors.initials: E
authors.lastname: Stephenson
authors.forename: Robert
authors.initials: R
authors.lastname: Collins
authors.forename: Heema
authors.initials: H
authors.lastname: Patel
authors.forename: Jane
authors.initials: J
authors.lastname: Trussler
authors.forename: Roaa
authors.initials: R
authors.lastname: Al-Bedaery
authors.forename: Pamela
authors.initials: P
authors.lastname: Renwick
authors.forename: Caroline
authors.initials: C
authors.lastname: Ogilvie
authors.forename: Robert
authors.initials: R
authors.lastname: Vaughan
authors.forename: Dusko
authors.initials: D
authors.lastname: Ilic
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods / Q000592:standards; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000276:immunology*; D005260:Female; D006680:HLA Antigens / Q000235:genetics; D006239:Haplotypes; D006650:Histocompatibility Testing / Q000379:methods; D006801:Humans; D006816:Huntington Disease / Q000175:diagnosis / Q000235:genetics; D008297:Male; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D019836:Preimplantation Diagnosis / Q000379:methods; D014015:Tissue Banks
chemical_list: D006680:HLA Antigens