title: An animal model study for repair of tracheal defects with autologous stem cells and differentiated chondrocytes from adipose-derived stem cells.
abstract: BACKGROUND
  Stenosis of trachea with mucosal and cartilage lesions is a challenging problem in tracheal surgery. Owing to ease of harvest and abundance, adipose-derived stem cells (ADSCs) are attractive and increasingly used in tissue engineering. The aim of this study was to evaluate the repair of trachea with autologous stem cells and differentiated chondrocytes from adipose-derived stem cells in an animal model.
  
  
  METHODS
  Six canine ADSCs were isolated and proliferated in monolayer culture and CD44; CD90 markers were investigated by flow cytometry. ADSCs were seeded in alginate beads and were differentiated into chondrocytes by TGF-β3. Cartilage-specific markers with reverse transcriptase polymerase chain reaction were demonstrated in differentiated cells. These differentiated cells and stem cells in alginate scaffold were separately transferred to a defect created in canine's trachea. After 8 weeks, the healing and cartilage formation in the trachea was evaluated by histological methods.
  
  
  RESULTS
  We identified formed cartilage pieces and chondrocytes with lacuna and extracellular matrix in defects implanted with differentiated cells, but in other groups, staining of the sections did not show the presence of cartilage in the engineered tracheal wall.
  
  
  CONCLUSIONS
  We showed that cartilage- engineered from differentiated adipose-derived stem cells in alginate biodegradable scaffold could repair tracheal cartilage defects.
authors.affiliation: Department of Anatomical Sciences and Molecular Biology Medical School, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Batool
authors.initials: B
authors.lastname: Hashemibeni
authors.forename: Vahid
authors.initials: V
authors.lastname: Goharian
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Esfandiari
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Sadeghi
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Fasihi
authors.forename: Razie
authors.initials: R
authors.lastname: Alipur
authors.forename: Ali
authors.initials: A
authors.lastname: Valiani
authors.forename: Masoud
authors.initials: M
authors.lastname: Ghorbani
authors.forename: Zahra Motavali
authors.initials: ZM
authors.lastname: Emami
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Shabani
authors.forename: Maryam
authors.initials: M
authors.lastname: Goharian
mesh_terms: D053674:Aggrecans / Q000378:metabolism; D000464:Alginates; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D019902:Chondrocytes / Q000378:metabolism / Q000637:transplantation*; D024043:Collagen Type II / Q000378:metabolism; D004195:Disease Models, Animal; D004285:Dogs; D005434:Flow Cytometry; D020723:Glucuronic Acid; D048091:Guided Tissue Regeneration / Q000379:methods*; D006603:Hexuronic Acids; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050151:Subcutaneous Fat / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014135:Tracheal Stenosis / Q000601:surgery*; D016896:Treatment Outcome
chemical_list: D053674:Aggrecans; D000464:Alginates; D015415:Biomarkers; D024043:Collagen Type II; D006603:Hexuronic Acids; D020723:Glucuronic Acid