title: Enhancing the antitumor cell proliferation and Cu(2+)-chelating effects of black soybeans through fermentation with Aspergillus awamori.
abstract: In the present study, black soybeans were fermented with Aspergillus awamori at 30°C for 3 days. The effect of fermentation on the antiproliferative effect against human colon cancer cells, Caco-2 and HT-29 as well as Cu(2+)-chelating effect of black soybeans was investigated. It was found that the water, 80% methanol or 80% ethanol extract of fermented black soybeans showed a significantly higher (P < 0.05) antiproliferative and Cu(2+)-chelating effect than did the respective extract of non-fermented black soybeans. Generally, the methanol extract and the ethanol extract of fermented black soybeans exerted higher antiproliferative effect on both Caco-2 and HT-29 cells. While water extract of fermented black soybeans showed the highest Cu(2+)-chelating effect among the various extracts examined. Taking into account of extraction yields further revealed that bioactive principles that exhibit Cu(2+)-chelating effect could be extracted to the largest extent with water as the extraction solvent. With same amount of sample, water extract obtained from fermented black soybeans possesses the highest Cu(2+)-chelating abilities.
authors.affiliation: Graduate Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan.
authors.forename: Yu-Fei
authors.initials: YF
authors.lastname: Chen
authors.forename: Ming-Lun
authors.initials: ML
authors.lastname: Chiang
authors.forename: Cheng-Chun
authors.initials: CC
authors.lastname: Chou
authors.forename: Yi-Chen
authors.initials: YC
authors.lastname: Lo
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001230:Aspergillus / Q000502:physiology*; D018938:Caco-2 Cells; D049109:Cell Proliferation / Q000187:drug effects*; D002614:Chelating Agents / Q000494:pharmacology*; D003300:Copper; D005285:Fermentation; D019073:HT29 Cells; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology; D013025:Glycine max / Q000378:metabolism* / Q000382:microbiology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002614:Chelating Agents; D010936:Plant Extracts; D003300:Copper