title: SUMOylation silences heterodimeric TASK potassium channels containing K2P1 subunits in cerebellar granule neurons.
abstract: The standing outward K(+) current (IKso) governs the response of cerebellar granule neurons to natural and medicinal stimuli including volatile anesthetics. We showed that SUMOylation silenced half of IKso at the surface of cerebellar granule neurons because the underlying channels were heterodimeric assemblies of K2P1, a subunit subject to SUMOylation, and the TASK (two-P domain, acid-sensitive K(+)) channel subunits K2P3 or K2P9. The heterodimeric channels comprised the acid-sensitive portion of IKso and mediated its response to halothane. We anticipate that SUMOylation also influences sensation and homeostatic mechanisms in mammals through TASK channels formed with K2P1.
authors.affiliation: Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA.
authors.forename: Leigh D
authors.initials: LD
authors.lastname: Plant
authors.forename: Leandro
authors.initials: L
authors.lastname: Zuniga
authors.forename: Dan
authors.initials: D
authors.lastname: Araki
authors.forename: Jeremy D
authors.initials: JD
authors.lastname: Marks
authors.forename: Steve A N
authors.initials: SA
authors.lastname: Goldstein
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002531:Cerebellum / Q000166:cytology* / Q000378:metabolism; D019281:Dimerization; D031541:Fluorescence Resonance Energy Transfer; D006221:Halothane / Q000494:pharmacology; D017403:In Situ Hybridization; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D018613:Microscopy, Confocal; D046912:Multiprotein Complexes / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D024683:Potassium Channels, Tandem Pore Domain / Q000378:metabolism*; D051381:Rats; D058207:Sumoylation / Q000502:physiology*
chemical_list: C487530:Kcnk1 protein, rat; C407082:Kcnk9 protein, rat; D046912:Multiprotein Complexes; D009419:Nerve Tissue Proteins; D024683:Potassium Channels, Tandem Pore Domain; C420132:potassium channel subfamily K member 3; D011188:Potassium; D006221:Halothane