title: Prognostic utility of autoantibodies to α-enolase and Hsp70 for cancer of the gingivo-buccal complex using immunoproteomics.
abstract: OBJECTIVE
  Studies from our laboratory have reported 14 tumor antigens that elicit an autoantibody response in patients with cancer of the gingivobuccal complex (GBC) In this study, utility of the autoantibody response has been evaluated for prognosis of cancer of the GBC.
  
  
  METHODS
  Autoantibody response was evaluated using immunoproteomics and the prognostic significance was assessed by Kaplan-Meier survival and multivariate analysis.
  
  
  RESULTS
  Autoantibody response against α-enolase isoforms a, b, and c and Hsp70 was detected in 27, 53, 64, and 26% of the 78 patients, respectively. Patients positive for autoantibody response to α-ENO and Hsp70 individually and in combination, showed significantly reduced disease-free survival (DFS) compared to those who do not show autoantibody response to either of them. Further the patients, who exhibit autoantibody response to α-ENO and Hsp70 in combination with nodal involvement and/or differentiation status, have significantly lowered DFS. The relative risk of recurrence is 3.41 for patients who exhibit autoantibody response to both the antigens.
  
  
  CONCLUSIONS
  Autoantibody response against α-ENO and Hsp70 provides an additional parameter and may be utilized along with nodal involvement and differentiation status for better prognosis of cancer of GBC.
authors.affiliation: Advanced Centre for Treatment, Research and Education in Cancer ACTREC, Tata Memorial Centre, Kharghar, Navi Mumbai 410210, India.
authors.forename: Atul
authors.initials: A
authors.lastname: Pranay
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Shukla
authors.forename: Sadhana
authors.initials: S
authors.lastname: Kannan
authors.forename: Siddhi A
authors.initials: SA
authors.lastname: Malgundkar
authors.forename: Rukmini B
authors.initials: RB
authors.lastname: Govekar
authors.forename: Asawari
authors.initials: A
authors.lastname: Patil
authors.forename: Shubhada V
authors.initials: SV
authors.lastname: Kane
authors.forename: Pankaj
authors.initials: P
authors.lastname: Chaturvedi
authors.forename: Anil K
authors.initials: AK
authors.lastname: D'Cruz
authors.forename: Surekha M
authors.initials: SM
authors.lastname: Zingde
mesh_terms: D000951:Antigens, Neoplasm; D001323:Autoantibodies / Q000276:immunology*; D018572:Disease-Free Survival; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D053208:Kaplan-Meier Estimate; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000276:immunology* / Q000401:mortality; D015999:Multivariate Analysis; D010751:Phosphopyruvate Hydratase / Q000276:immunology* / Q000378:metabolism; D011379:Prognosis; D040901:Proteomics; D012008:Recurrence; D012307:Risk Factors
chemical_list: D000951:Antigens, Neoplasm; D001323:Autoantibodies; D018840:HSP70 Heat-Shock Proteins; D010751:Phosphopyruvate Hydratase