title: Low-level laser therapy (LLLT) combined with swimming training improved the lipid profile in rats fed with high-fat diet.
abstract: Obesity and associated dyslipidemia is the fastest growing health problem throughout the world. The combination of exercise and low-level laser therapy (LLLT) could be a new approach to the treatment of obesity and associated disease. In this work, the effects of LLLT associated with exercises on the lipid metabolism in regular and high-fat diet rats were verified. We used 64 rats divided in eight groups with eight rats each, designed: SC, sedentary chow diet; SCL, sedentary chow diet laser, TC, trained chow diet; TCL, trained chow diet laser; SH, sedentary high-fat diet; SHL, sedentary high-fat diet laser; TH, trained high-fat diet; and THL, trained high-fat diet laser. The exercise used was swimming during 8 weeks/90 min daily and LLLT (GA-Al-As, 830 nm) dose of 4.7 J/point and total energy 9.4 J per animal, applied to both gastrocnemius muscles after exercise. We analyzed biochemical parameters, percentage of fat, hepatic and muscular glycogen and relative mass of tissue, and weight percentage gain. The statistical test used was ANOVA, with post hoc Tukey-Kramer for multiple analysis between groups, and the significant level was p < 0.001, p < 0.01, and p < 0.05. LLLT decreased the total cholesterol (p < 0.05), triglycerides (p < 0.01), low-density lipoprotein cholesterol (p < 0.05), and relative mass of fat tissue (p < 0.05), suggesting increased metabolic activity and altered lipid pathways. The combination of exercise and LLLT increased the benefits of exercise alone. However, LLLT without exercise tended to increase body weight and fat content. LLLT may be a valuable addition to a regimen of diet and exercise for weight reduction and dyslipidemic control.
authors.affiliation: Optics Group from Institute of Physics of São Carlos (IFSC), University of São Paulo (USP), São Carlos, Brazil. spydera@ig.com.br
authors.forename: Antonio E
authors.initials: AE
authors.lastname: Aquino
authors.forename: Marcela
authors.initials: M
authors.lastname: Sene-Fiorese
authors.forename: Fernanda R
authors.initials: FR
authors.lastname: Paolillo
authors.forename: Fernanda O
authors.initials: FO
authors.lastname: Duarte
authors.forename: Jorge C
authors.initials: JC
authors.lastname: Oishi
authors.forename: Airton A
authors.initials: AA
authors.lastname: Pena
authors.forename: Ana C G O
authors.initials: AC
authors.lastname: Duarte
authors.forename: Michael R
authors.initials: MR
authors.lastname: Hamblin
authors.forename: Vanderlei S
authors.initials: VS
authors.lastname: Bagnato
authors.forename: Nivaldo A
authors.initials: NA
authors.lastname: Parizotto
mesh_terms: D000818:Animals; D003131:Combined Modality Therapy; D059305:Diet, High-Fat / Q000009:adverse effects; D050171:Dyslipidemias / Q000097:blood / Q000628:therapy; D050356:Lipid Metabolism / Q000528:radiation effects*; D008055:Lipids / Q000097:blood; D008112:Liver Glycogen / Q000378:metabolism / Q000528:radiation effects; D028022:Low-Level Light Therapy; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism / Q000528:radiation effects; D009765:Obesity / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013550:Swimming / Q000502:physiology
chemical_list: D008055:Lipids; D008112:Liver Glycogen