title: Detecting and quantifying p53 isoforms at mRNA level in cell lines and tissues.
abstract: The TP53 gene expresses at least nine different mRNA variants (p53 isoform mRNAs), including the one encoding the canonical p53 tumor suppressor protein. We have developed scientific tools to specifically detect and quantify p53 isoform expression at mRNA level by nested RT-PCR (reverse transcription-polymerase chain reaction) and quantitative real-time RT-PCR (RT-qPCR using the TaqMan(®) chemistry). Here, we describe these two methods, while highlighting essential points with regard to the analysis of p53 isoform mRNA expression.
authors.affiliation: CR-UK Cell Transformation Research Group, Centre for Oncology and Molecular Medicine, Ninewells Hospital, University of Dundee, Dundee, UK.
authors.forename: Marie P
authors.initials: MP
authors.lastname: Khoury
authors.forename: Virginie
authors.initials: V
authors.lastname: Marcel
authors.forename: Kenneth
authors.initials: K
authors.lastname: Fernandes
authors.forename: Alexandra
authors.initials: A
authors.lastname: Diot
authors.forename: David P
authors.initials: DP
authors.lastname: Lane
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Bourdon
mesh_terms: D045744:Cell Line, Tumor; D016158:Genes, p53; D006801:Humans; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D020033:Protein Isoforms; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53