title: Phytochemical analysis and toxicity investigation of stem bark of Scutia buxifolia Reissek.
abstract: Phytochemical analysis of lyophilised aqueous extract of the stem bark of Scutia buxifolia (SBSB) was carried out by determining total phenolics (0.280 ± 0.02 mg of gallic acid equivalents/g of extract), flavonoids (17.42 ± 2.95 mg of quercetin equivalents/g of extract) and tannins (1.28 ± 0.15 mg of catechin equivalents/g of extract) contents followed by a high-performance liquid chromatography/diode array detection (HPLC/DAD) analysis. The HPLC profile showed caffeic acid, being the major constituent of SBSB (247.21 ± 2.17 mg g⁻¹ of extract). The antioxidant scavenging capacity of SBSB was determined by 2,2-diphenyl-2-picrylhydrazyl (DPPH) assay. The antioxidant power of SBSB was comparable with that of the antioxidant ascorbic acid. Acute toxicity was assayed in rats whereas catalase activity and malondialdehyde production were determined in rats' liver. The SBSB showed safety in the dose tested. This report is the first realised in animals for S. buxifolia.
authors.affiliation: a Phytochemical Research Laboratory, Department of Industrial Pharmacy , Federal University of Santa Maria , Build 26, Room 1115 , Santa Maria , CEP 97105-900 , Brazil.
authors.forename: Robson Borba
authors.initials: RB
authors.lastname: de Freitas
authors.forename: Isabel Cristina
authors.initials: IC
authors.lastname: da Costa Araldi
authors.forename: Aline Augusti
authors.initials: AA
authors.lastname: Boligon
authors.forename: Thiele Faccin
authors.initials: TF
authors.lastname: de Brum
authors.forename: Bruno Tomazele
authors.initials: BT
authors.lastname: Rovani
authors.forename: Mariana
authors.initials: M
authors.lastname: Piana
authors.forename: Marina
authors.initials: M
authors.lastname: Zadra
authors.forename: Margareth Linde
authors.initials: ML
authors.lastname: Athayde
authors.forename: Liliane
authors.initials: L
authors.lastname: de Freitas Bauermann
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D002109:Caffeic Acids / Q000737:chemistry; D002392:Catechin / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000737:chemistry; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D010636:Phenols / Q000737:chemistry; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D018547:Plant Stems / Q000737:chemistry*; D051381:Rats; D027823:Rhamnaceae / Q000737:chemistry*; D013634:Tannins / Q000737:chemistry
chemical_list: D000975:Antioxidants; D002109:Caffeic Acids; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D013634:Tannins; D002392:Catechin; D001205:Ascorbic Acid; C040048:caffeic acid