title: [Role of heme oxygenase-1 in the apoptosis of mesenchymal stem cells].
abstract: OBJECTIVE
  To explore the role of heme oxygenase-1 (HO-1) in the apoptosis of mesenchymal stem cells (MSCs).
  
  
  METHODS
  The method of reverse transcription PCR (RT-PCR) was employed to detect the expression of HO-1 mRNA in bone marrow MSCs. Morphologic observations, flow cytometry Annexin-V and DNA analysis were used to detect apoptosis and determine the content of DNA of MSCs after a 12-hour treatment of Znpp-IX, a Ho-1 special inhibitor.
  
  
  RESULTS
  HO-1 mRNA was expressed in normal bone marrow MSCs. After culturing Znpp-IX for 12 h, MSCs showed volume reduction and membrane shrinkage. The cells of apoptotic Annexin V(+)/PI(-) increased significantly (61.21% ± 5.28% vs 7.48% ± 0.69%, P < 0.05). The peak of apoptosis was distinct on the distribution graph of DNA (21.36% ± 0.49% vs 1.02% ± 0.05%, P < 0.05).
  
  
  CONCLUSIONS
  HO-1 plays an anti-apoptotic role in human bone marrow mesenchymal stem cells.
authors.affiliation: Department of Hematology, Lanzhou General Hospital, Lanzhou, China. baihai98@tom.com
authors.forename: Hai
authors.initials: H
authors.lastname: Bai
authors.forename: Cun-bang
authors.initials: CB
authors.lastname: Wang
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Ma
authors.forename: Rui
authors.initials: R
authors.lastname: Xi
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jin-mao
authors.initials: JM
authors.lastname: Zhou
mesh_terms: D017209:Apoptosis; D002454:Cell Differentiation; D002478:Cells, Cultured; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1