title: CD24 expression is associated with progression of gastric cancer.
abstract: OBJECTIVE
  Mortality rates due to gastric cancer are high in Japan. To improve patient prognosis, new biomarkers for diagnosis and treatment are urgently required. In this study we investigated the role of CD24, a cell adhesion glycoprotein implicated in tumor cell proliferation, which is used as a prognostic marker in various cancers.
  
  
  METHODS
  We analyzed CD24 expression in 173 gastric adenocarcinomas by immunohistochemistry and compared the data with clinicopathological parameters and patient overall survival. Furthermore, we performed Western blotting analysis of CD24 in six human gastric adenocarcinoma cell lines, Kato III, MKN1, MKN28, MKN45, MKN74, and HGC-27.
  
  
  RESULTS
  CD24 up-regulation was significantly correlated with depth of invasion (p=0.005) and pathological high stages (p=0.043). We observed a relationship between high CD24 expression and lymph node metastasis, venous invasion and lymphatic invasion. CD24 expression tended to be higher in cell lines derived from differentiated gastric carcinoma, including those derived from lymph node metastasis.
  
  
  CONCLUSIONS
  Our study suggests that gastric cancer patients with high CD24 expression should be closely monitored for recurrence following resections. CD24 expression is a potential biomarker for gastric cancer prognosis and provides a new molecular target for therapeutic strategies.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Takahashi
authors.forename: Masanobu
authors.initials: M
authors.lastname: Nakajima
authors.forename: Hideo
authors.initials: H
authors.lastname: Ogata
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Domeki
authors.forename: Kichiro
authors.initials: K
authors.lastname: Ohtsuka
authors.forename: Keisuke
authors.initials: K
authors.lastname: Ihara
authors.forename: Eigo
authors.initials: E
authors.lastname: Kurayama
authors.forename: Satoru
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Kinro
authors.initials: K
authors.lastname: Sasaki
authors.forename: Kazuto
authors.initials: K
authors.lastname: Miyachi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kato
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D015153:Blotting, Western; D051927:CD24 Antigen / Q000378:metabolism*; D045744:Cell Line, Tumor; D018450:Disease Progression; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007564:Japan; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; D051927:CD24 Antigen