title: Formation of embryoid bodies from human pluripotent stem cells using AggreWell™ plates.
abstract: Many human embryonic stem (hES) and induced pluripotent stem (hiPS) cell differentiation protocols begin with the formation of three-dimensional aggregates of cells called embryoid bodies (EBs). Traditional EB formation methods result in a heterogeneous population of EB sizes and shapes, which then undergo heterogeneous differentiation efficiencies. AggreWell(TM)400 and AggreWell(TM)800 use the spin-EB method to force the aggregation of a defined number of cells, thereby controlling EB size and generating a population of uniform EBs. Moreover, the dense array of microwells on the bottom surface of AggreWell(TM)400 provide for the rapid and simple production of thousands of EBs at a time.
authors.affiliation: STEMCELL Technologies Inc, Vancouver, BC, Canada. Jennifer.antonchuk@stemcell.com
authors.forename: Jennifer
authors.initials: J
authors.lastname: Antonchuk
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D058732:Embryoid Bodies / Q000166:cytology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D013535:Suspensions
chemical_list: D013535:Suspensions