title: Glucan of a somatic hybrid mushroom, pfls1h: structural characterization and study of immunological activities.
abstract: A water-soluble glucan (PS-I) was isolated from the aqueous extract of the fruit bodies of a hybrid mushroom, pfls1h of Pleurotus florida and Lentinus squarrosulus (Mont.) Singer. Structural characterization of PS-I was carried out using total hydrolysis, methylation analysis, periodate oxidation, and NMR experiments ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HSQC, and HMBC). Methylation analysis revealed that PS-I was composed of (1→3, 6), (1→3), (1→6)-linked and terminal β-d-glucopyranosyl residues in a relative proportion of approximately 1:1:1:1. The repeating unit of the glucan consists of a backbone chain of two (1→6)-β-d-glucopyranosyl residues, one of which is branched at O-3 position with (1→3)-β-d-glucopyranosyl and terminated with a β-d-glucopyranosyl residue. Study of immunological activity revealed that PS-I stimulates the splenocytes, thymocytes and macrophages.
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Ipsita K
authors.initials: IK
authors.lastname: Sen
authors.forename: Praloy K
authors.initials: PK
authors.lastname: Maji
authors.forename: Birendra
authors.initials: B
authors.lastname: Behera
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Maiti
authors.forename: Pijush
authors.initials: P
authors.lastname: Mallick
authors.forename: Samir R
authors.initials: SR
authors.lastname: Sikdar
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D007155:Immunologic Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D008745:Methylation; D051379:Mice; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000378:metabolism; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000737:chemistry; D013154:Spleen / Q000166:cytology; D060168:Thymocytes / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D013950:Thymus Gland / Q000166:cytology
chemical_list: D062610:Fungal Polysaccharides; D007155:Immunologic Factors; D010504:Periodic Acid; D009569:Nitric Oxide; C009288:metaperiodate