title: Antibody to heat shock protein 70 (HSP70) inhibits human T-cell lymphoptropic virus type I (HTLV-I) production by transformed rabbit T-cell lines.
abstract: Adult T cell leukemia is a fatal malignant transformation caused by the human T-cell lymphoptropic virus type I (HTLV-I). HTLV-I is only associated with the development of this disease in a small percentage of infected individuals. Using two rabbit transformed T-cell lines; RH/K30 (asymptomatic) and RH/K34 (leukemogenic), we have investigated the expression of heat shock proteins (HSP) 90 and 70 and the role of anti-HSPs antibodies on virus production. HSPs surface expression was higher on RH/K34 than RH/K30 cells. Heat treatment of cells increased the expression of HSPs proteins and virus production; HSPs augmentation was stabilized after 12 h and virus production reached a maximum between 8 h-12 h then returned to normal level after 24 h of culture. Incubation of cells only with rabbit anti-HSP 70 antibodies prevented virus production specifically in the leukemogenic cell line. The results indicate a relationship between HSP 70 and virus production.
authors.affiliation: Faculty of Science, University of Damascus, Damascus, Syria. hafa2@live.fr
authors.forename: Hanan
authors.initials: H
authors.lastname: Fallouh
authors.forename: Wahib
authors.initials: W
authors.lastname: Mahana
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002460:Cell Line; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D015368:Human T-lymphotropic virus 1 / Q000254:growth & development* / Q000276:immunology / Q000472:pathogenicity; D023421:Models, Animal; D011817:Rabbits; D013312:Stress, Physiological; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000821:virology*
chemical_list: D000906:Antibodies; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins