title: In-depth proteomic analysis of banana (Musa spp.) fruit with combinatorial peptide ligand libraries.
abstract: Musa ssp. is among the world's leading fruit crops. Although a strong interest on banana biochemistry exists in the scientific community, focused on metabolite composition, proteins have been scarcely investigated even if they play an important role in food allergy and stability, are a source of biologically active peptides, and can provide information about nutritional aspects of this fruit. In this work we have employed the combinatorial peptide ligand libraries after different types of protein extractions, for searching the very low-abundance proteins in banana. The use of advanced MS techniques and Musa ssp. mRNAs database in combination with the Uniprot_viridiplantae database allowed us to identify 1131 proteins. Among this huge amount of proteins we found several already known allergens such as Mus a 1, pectinesterase, superoxide dismutase, and potentially new allergens. Additionally several enzymes involved in degradation of starch granules and strictly correlated to ripening stage were identified. This is the first in-depth exploration of the banana fruit proteome and one of the largest descriptions of the proteome of any vegetable system.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Alcalá, Alcalá de Henares, Madrid, Spain.
authors.forename: Clara
authors.initials: C
authors.lastname: Esteve
authors.forename: Alfonsina
authors.initials: A
authors.lastname: D'Amato
authors.forename: María Luisa
authors.initials: ML
authors.lastname: Marina
authors.forename: María Concepción
authors.initials: MC
authors.lastname: García
authors.forename: Pier Giorgio
authors.initials: PG
authors.lastname: Righetti
mesh_terms: D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D028521:Musa / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019151:Peptide Library; D010940:Plant Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D019151:Peptide Library; D010940:Plant Proteins; D020543:Proteome