title: Identification of feline panleukopenia virus proteins expressed in Purkinje cell nuclei of cats with cerebellar hypoplasia.
abstract: Parvoviruses depend on initiation of host cell division for their replication. Undefined parvoviral proteins have been detected in Purkinje cells of the cerebellum after experimental feline panleukopenia virus (FPV) infection of neonatal kittens and in naturally occurring cases of feline cerebellar hypoplasia. In this study, a parvoviral protein in the nucleus of Purkinje cells of kittens with cerebellar hypoplasia was shown by immunoprecipitation to be the FPV viral capsid protein VP2. In PCR-confirmed, FPV-associated feline cerebellar hypoplasia, expression of the FPV VP2 protein was demonstrated by immunohistochemistry in Purkinje cell nuclei in 4/10 cases and expression of the FPV non-structural protein NS1 was demonstrated in Purkinje cell nuclei in 5/10 cases. Increased nuclear ERK1 expression was observed in several Purkinje cells in 1/10 kittens. No expression of the G1 and S mitotic phase marker proliferating cell nuclear antigen (PCNA) was evident in Purkinje cell nuclei. These results support the hypothesis that FPV is able to proceed far into its replication cycle in post-mitotic Purkinje cells.
authors.affiliation: Laboratory of Anatomy, Biomechanics and Organogenesis, Faculty of Medicine, Université Libre de Bruxelles, 808 route de Lennik, B-1070 Bruxelles, Belgium. lcponce@ulb.ac.be
authors.forename: Luc
authors.initials: L
authors.lastname: Poncelet
authors.forename: Céline
authors.initials: C
authors.lastname: Héraud
authors.forename: Marie
authors.initials: M
authors.lastname: Springinsfeld
authors.forename: Kunie
authors.initials: K
authors.lastname: Ando
authors.forename: Anna
authors.initials: A
authors.lastname: Kabova
authors.forename: Andreas
authors.initials: A
authors.lastname: Beineke
authors.forename: Dominique
authors.initials: D
authors.lastname: Peeters
authors.forename: Anne
authors.initials: A
authors.lastname: Op De Beeck
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Brion
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000918:Antibody Specificity; D002371:Cat Diseases / Q000473:pathology / Q000821:virology*; D002415:Cats; D002531:Cerebellum / Q000002:abnormalities* / Q000166:cytology / Q000378:metabolism / Q000821:virology; D002658:Developmental Disabilities / Q000378:metabolism / Q000821:virology; D028301:Feline Panleukopenia Virus / Q000235:genetics / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D007150:Immunohistochemistry / Q000662:veterinary; D009421:Nervous System Malformations / Q000378:metabolism / Q000662:veterinary* / Q000821:virology; D011689:Purkinje Cells / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014764:Viral Proteins