title: Oxidative stress and antioxidant activity of female rat liver tissue after sevoflurane anaesthesia: young versus old.
abstract: OBJECTIVE
  The aim of our study was to assess the evidence of oxidative stress in the rat liver tissue by studying enzymes, such as nitric oxide synthase (NOS), superoxide dismutase (SOD), glutathione-S transferase (GST) activity, and thiobarbutiric acid-reactive substances (TBARS) levels in young versus old female rats after sevoflurane anaesthesia.
  
  
  METHODS
  The study involved 28 female Wistar Albino rats. The rats were divided into the two groups [(Group I, n=14): Young sevoflurane group (Group I-YS, n=7); Young control group (Group I-YC, n=7)], [(Group II, n=14): Old sevoflurane group (Group II-OS, n=7); Old control group (Group II-OC, n=7)]. Sevoflurane was administered at 2 % volume inspiratory concentration, 6 L.min-1 in 100 % O2 for 2 hours. The control groups were not subjected to any procedures. Accordingly, GST, SOD, and NOS enzyme activity and TBARS level, were studied in the liver tissue samples of the rats to determine the presence of oxidative stress (OS) and antioxidant activity.
  
  
  RESULTS
  Following administration of sevoflurane anaesthesia; GST, SOD enzyme activity and TBARS level was significantly higher in the Group I-YS than in the Group I-YC and in the Group II-OS than in the Group II-OC. There was no difference between the groups when the mean NOS levels were compared.
  
  
  CONCLUSIONS
  Although the results of our study are similar to the previous indicating that sevoflurane has the capacity to induce the oxidative stress; a new data has been recorded that sevoflurane has a similar effect on the OS level in aged and young female rat (Tab. 1, Fig. 4, Ref. 48).
authors.affiliation: Gazi University Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Ankara, Turkey. marslan36@hotmail.com
authors.forename: M
authors.initials: M
authors.lastname: Arslan
authors.forename: B
authors.initials: B
authors.lastname: Isik
authors.forename: M
authors.initials: M
authors.lastname: Kavutcu
authors.forename: O
authors.initials: O
authors.lastname: Kurtipek
mesh_terms: D000375:Aging / Q000378:metabolism*; D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D005260:Female; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008738:Methyl Ethers / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D000077149:Sevoflurane; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D018685:Anesthetics, Inhalation; D000975:Antioxidants; D008738:Methyl Ethers; D017392:Thiobarbituric Acid Reactive Substances; D000077149:Sevoflurane; D019001:Nitric Oxide Synthase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase