title: Iodide transporters expression in early human invasive trophoblast.
abstract: BACKGROUND
  The placenta plays an essential role in the fetomaternal exchanges of iodine and thyroid hormones. Propylthiouracil (PTU) is presently considered to be the treatment of choice for hyperthyroidism during the first trimester of pregnancy. Little is known on the expression of iodide transporters in invasive human trophoblast and the possible effect of PTU on this early phase of human placental development.
  
  
  OBJECTIVE
  To analyze during early pregnancy expression of sodium/iodide symporter (NIS) and pendrin at the feto-maternal interface in situ in first trimester placentas, in vitro during human trophoblastic cell differentiation in presence or not of PTU.
  
  
  METHODS
  NIS and pendrin immunodetection were performed on 8-10 WG placental tissue sections and in primary cultures of first trimester placenta trophoblastic cells, which differentiate in vitro into syncytiotrophoblast or invasive extravillous cytotrophoblasts (EVCT). The effect of PTU (1 mM) was tested in EVCT on iodide transporters expression, cell invasion, and hCG secretion.
  
  
  RESULTS
  NIS and pendrin were present in early human trophoblast at the maternofetal interface. Their expression was modulated with in vitro trophoblast differentiation. Early invasive EVCT were characterized by higher expression of NIS than pendrin. In vitro PTU did modify significantly neither EVCT iodide transporters expression nor EVCT biological functions: i.e. invasive properties and hCG secretion.
  
  
  CONCLUSIONS
  This study reveals that NIS is highly expressed in early human trophoblast at the feto-maternal interface. PTU has no effect on early human trophoblast invasion.
authors.affiliation: INSERM, UMR-S 767, Faculté des Sciences Pharmaceutiques et Biologiques, Paris, France.
authors.forename: S A
authors.initials: SA
authors.lastname: Degrelle
authors.forename: J
authors.initials: J
authors.lastname: Guibourdenche
authors.forename: F
authors.initials: F
authors.lastname: Galland
authors.forename: J M
authors.initials: JM
authors.lastname: Bidart
authors.forename: T
authors.initials: T
authors.lastname: Fournier
authors.forename: D
authors.initials: D
authors.lastname: Evain-Brion
mesh_terms: D013956:Antithyroid Agents / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical; D005260:Female; D006801:Humans; D007455:Iodine / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D008954:Models, Biological; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000235:genetics* / Q000378:metabolism; D061251:Primary Cell Culture; D011441:Propylthiouracil / Q000494:pharmacology; D000076988:Sulfate Transporters; D027981:Symporters / Q000235:genetics* / Q000378:metabolism; D014327:Trophoblasts / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology*; D000099209:Sodium-Iodide Symporters
chemical_list: D013956:Antithyroid Agents; D007455:Iodine; D026901:Membrane Transport Proteins; D011441:Propylthiouracil; D000076988:Sulfate Transporters; D027981:Symporters; D000099209:Sodium-Iodide Symporters; C085609:SLC26A4 protein, human