title: Prevalence of human parechoviruses in central nervous system infections in children: a retrospective study in Shanghai, China.
abstract: Human parechoviruses (HPeV) are associated with central nervous system (CNS) infections and sepsis-like illnesses. However, data from China are not available. The aim of this study was to determine the prevalence, age, and seasonal distributions and genotypes of HPeV infections in children with CNS related disease in Shanghai, China. Of 776 cerebrospinal fluid (CSF) samples collected from children with CNS-related diseases under the age of 16 years during the years 2008-2011, 68 (9%) were identified to be HPeV positive. The annual prevalence varied remarkably: 1% (2/153) in 2008, 7% (12/177) in 2009, 15% (23/153) in 2010, and 11% (31/293) in 2011. The virus was detected in all age groups of children ranging from 2 days to 13 years and the median age was 14 months. Of the 31 positive samples that were genotyped successfully, 28 were HPeV1 and 3 were HPeV3. This study provided data on the molecular epidemiology of HPeV infections in CNS-related diseases in Shanghai, China and suggest that the screening for HPeV by PCR should be included in the routine viral testing of CSF.
authors.affiliation: Pediatric Institute, Children's Hospital of Fudan University, Shanghai 201102, China.
authors.forename: Huaqing
authors.initials: H
authors.lastname: Zhong
authors.forename: Yong
authors.initials: Y
authors.lastname: Lin
authors.forename: Liyun
authors.initials: L
authors.lastname: Su
authors.forename: Lingfeng
authors.initials: L
authors.lastname: Cao
authors.forename: Menghua
authors.initials: M
authors.lastname: Xu
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
mesh_terms: D000293:Adolescent; D000367:Age Factors; D002494:Central Nervous System Infections / Q000453:epidemiology* / Q000821:virology; D002555:Cerebrospinal Fluid / Q000821:virology; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D030061:Parechovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000453:epidemiology* / Q000821:virology; D015995:Prevalence; D012189:Retrospective Studies; D012621:Seasons