title: Evaluation of in vitro antioxidant potency of exopolysaccharide from endophytic Fusarium solani SD5.
abstract: A potent endophytic fungus, Fusarium solani SD5 was used for exopolysaccharide (EPS) production. The isolated EPS were purified and major EPS fraction (PS-I); rhamno galactan was used to evaluate anti oxidant activities in vitro. EPS (PS-I) showed significant free radical scavenging effect on DPPH (1,1-diphenyl-2-picrylhydrazyl) and scavenging potency is indicated by IC(50) value 578.541 ± 33.256 μg/ml. EPS (PS-I) significantly induced antioxidant parameters of peritoneal macrophage cells at a concentration dependent manner and at 500 μg/ml it showed maximum protective effect against free radicals [malondialdehyde (MDA) 0.178 ± 0.015; super oxide dismutase (SOD) 41.287 ± 1.051; glutathione peroxidase (GPx) 30.182 ± 1.237; reduced glutathione (GSH) 56.892 ± 1.272; oxidized glutathione (GSSG) 8.458 ± 0.768]. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide] cytotoxicity assay indicated that EPS (PS-I) had no significant cytotoxic effect (concentration up to 500 μg/ml) on macrophage cells. Present findings suggested that the EPS (PS-I) may become a potential nontoxic exogenous antioxidant.
authors.affiliation: Microbiology Laboratory, Department of Botany and Forestry, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Subhadip
authors.initials: S
authors.lastname: Mahapatra
authors.forename: Debdulal
authors.initials: D
authors.lastname: Banerjee
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D060026:Endophytes / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D005609:Free Radicals / Q000737:chemistry; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D005670:Fusarium / Q000737:chemistry*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000502:physiology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D010851:Picrates / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism; D013778:Tetrazolium Salts / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D062610:Fungal Polysaccharides; D010851:Picrates; D013778:Tetrazolium Salts; D013844:Thiazoles; D008315:Malondialdehyde; C004931:1,1-diphenyl-2-picrylhydrazyl; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; C022616:thiazolyl blue; D005978:Glutathione; D001205:Ascorbic Acid