title: Spatially resolved electrochemical sensing of chemical gradients.
abstract: Chemical gradients drive a diverse set of biological processes ranging from nerve transduction to ovulation. At present, the most common method for quantifying chemical gradients is microscopy. Here, a new concept for probing spatial and temporal chemical gradients is reported that uses a multi-layer microfluidic device to measure analyte concentration as a function of lateral position in a microfluidic channel using electrochemistry in a format that is readily adaptable to multi-analyte sensing.
authors.affiliation: Department of Chemistry, Colorado State University, 1872 Campus Delivery, Fort Collins, Colorado 80523, USA.
authors.forename: Meghan M
authors.initials: MM
authors.lastname: Mensack
authors.forename: John B
authors.initials: JB
authors.lastname: Wydallis
authors.forename: N Scott
authors.initials: NS
authors.lastname: Lynn
authors.forename: David S
authors.initials: DS
authors.lastname: Dandy
authors.forename: Charles S
authors.initials: CS
authors.lastname: Henry
mesh_terms: D004298:Dopamine / Q000032:analysis; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019793:Fluorescein / Q000737:chemistry; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods
chemical_list: D019793:Fluorescein; D004298:Dopamine