title: High-throughput RNA in situ hybridization in mouse retina.
abstract: The introduction of large-scale gene expression profiling studies has greatly increased the need to rapidly obtain high-quality cellular expression patterns of genes found to exhibit differential expression. The use of large-scale nonradioactive RNA in situ hybridization makes this possible, and greatly increases the general usefulness of this data. Here, we describe protocols for parallel analysis of up to 50 different gene-specific probes in mouse retinal sections.
authors.affiliation: The Salomon H. Snyder Department of Neuroscience, John Hopkins University School of Medicine, Baltimore, MD, USA. sblack@jhmi.edu
authors.forename: Seth
authors.initials: S
authors.lastname: Blackshaw
mesh_terms: D000818:Animals; D005629:Frozen Sections / Q000379:methods; D057166:High-Throughput Screening Assays / Q000379:methods*; D017403:In Situ Hybridization / Q000379:methods*; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000032:analysis* / Q000235:genetics; D012160:Retina / Q000378:metabolism*; D016707:Tissue Fixation / Q000379:methods
chemical_list: D012313:RNA