title: Development of a sandwich enzyme-linked immunosorbent assay to detect and measure serum levels of canine ferritin.
abstract: We established a homologous sandwich enzyme-linked immunosorbent assay (ELISA) to measure serum levels of canine ferritin. Our assay uses a rabbit anti-canine heart ferritin polyclonal antibody, and canine heart ferritin as a standard. Serum ferritin concentration in healthy dogs (n=163) was 789 ± 284 ng/ml (mean ± standard deviation), a value higher than reported previously. Confidence levels relating to repeatability, dilution and recovery for this method were high. Therefore, we believe our developed sandwich ELISA will be effective in evaluating serum levels of canine ferritin.
authors.affiliation: Department of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University, 23-35-1 Higashi, Towada, Aomori, Japan. chikazaw@vmas.kitasato-u.ac.jp
authors.forename: Seishiro
authors.initials: S
authors.lastname: Chikazawa
authors.forename: Yasutomo
authors.initials: Y
authors.lastname: Hori
authors.forename: Fumio
authors.initials: F
authors.lastname: Hoshi
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Kanai
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Ito
authors.forename: Jun
authors.initials: J
authors.lastname: Sato
authors.forename: Koichi
authors.initials: K
authors.lastname: Orino
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Watanabe
authors.forename: Sei-ichi
authors.initials: S
authors.lastname: Higuchi
mesh_terms: D000818:Animals; D004285:Dogs / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D005293:Ferritins / Q000097:blood*; D008297:Male; D015203:Reproducibility of Results
chemical_list: D005293:Ferritins