title: [Effects of EGCG on the nasopharyngeal carcinoma cell line CNE-2 and the expression of related gene].
abstract: OBJECTIVE
  To study the effects of epigallocatechin-3-gallate (EGCG) on proliferation and apoptosis of nasopharyngeal carcinoma CNE-2 cell line and analyze the expression of Bcl-2, Bax and Caspase-3 in the cell line which treated with EGCG.
  
  
  METHODS
  MTT assay and flow cytometry were used to analyze cell proliferation and cell cycle. Hoechst33258 fluorescence staining was adopted to study cell apoptosis. RT-PCR was used to detect the expression of Bcl-2, Bax, Caspase-3.
  
  
  RESULTS
  EGCG could significantly inhibit proliferation of CNE-2 cell line and induce its apoptosis with dose-independent relationship. EGCG could suppress the expression of Bcl-2 and induce expression of Bax, Caspase-3.
  
  
  CONCLUSIONS
  EGCG in vitro has efficacy of anti-nasopharyngeal carcinoma cells, which may be through regulating the expression of cell proliferation and apoptosis genes involved.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Affiliated Hospital of Guilin Medical College, Guilin, 541001, China. kongzhongyuilql@163.com
authors.forename: Xun
authors.initials: X
authors.lastname: Lei
authors.forename: Zhongyu
authors.initials: Z
authors.lastname: Kong
authors.forename: Caiwen
authors.initials: C
authors.lastname: Fan
authors.forename: Shijiang
authors.initials: S
authors.lastname: Yi
authors.forename: Qiu
authors.initials: Q
authors.lastname: Xiang
authors.forename: Qianghe
authors.initials: Q
authors.lastname: Liu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002277:Carcinoma; D053148:Caspase 3 / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494826:BAX protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D002392:Catechin; C045651:epigallocatechin gallate; C505407:CASP3 protein, human; D053148:Caspase 3