title: Exoglucanase-encoding genes from three Wickerhamomyces anomalus killer strains isolated from olive brine.
abstract: Wickerhamomyces anomalus killer strains are important for fighting pathogenic yeasts and for controlling harmful yeasts and bacteria in the food industry. Targeted disruption of key genes in β-glucan synthesis of a sensitive Saccharomyces cerevisiae strain conferred resistance to the toxins of W. anomalus strains BS91, BCA15 and BCU24 isolated from olive brine. Competitive inhibition of the killing activities by laminarin and pustulan refer to β-1,3- and β-1,6-glucans as the main primary toxin targets. The extracellular exoglucanase-encoding genes WaEXG1 and WaEXG2 from the three strains were sequenced and were found to display noticeable similarities to those from known potent W. anomalus killer strains.
authors.affiliation: DISPA, Sezione di Tecnologia e Microbiologia degli Alimenti, University of Catania, Italy.
authors.forename: Serena
authors.initials: S
authors.lastname: Muccilli
authors.forename: Sabrina
authors.initials: S
authors.lastname: Wemhoff
authors.forename: Cristina
authors.initials: C
authors.lastname: Restuccia
authors.forename: Friedhelm
authors.initials: F
authors.lastname: Meinhardt
mesh_terms: D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D031658:Olea / Q000382:microbiology*; D004718:Saccharomycetales / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D012492:Salts; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D012492:Salts; C017082:brine; D006026:Glycoside Hydrolases