title: The transcriptional regulator lola is required for stem cell maintenance and germ cell differentiation in the Drosophila testis.
abstract: Stem cell behavior is regulated by extrinsic signals from specialized microenvironments, or niches, and intrinsic factors required for execution of context-appropriate responses to niche signals. Here we show that function of the transcriptional regulator longitudinals lacking (lola) is required cell autonomously for germline stem cell and somatic cyst stem cell maintenance in the Drosophila testis. In addition, lola is also required for proper execution of key developmental transitions during male germ cell differentiation, including the switch from transit amplifying progenitor to spermatocyte growth and differentiation, as well as meiotic cell cycle progression and spermiogenesis. Different lola isoforms, each having unique C-termini and zinc finger domains, may control different aspects of proliferation and differentiation in the male germline and somatic cyst stem cell lineages.
authors.affiliation: Department of Developmental Biology, Stanford University School of Medicine, 279 Campus Drive, Beckman Center B300, Stanford, CA 94305-5329, USA.
authors.forename: Erin L
authors.initials: EL
authors.lastname: Davies
authors.forename: Jaclyn G Y
authors.initials: JG
authors.lastname: Lim
authors.forename: William J
authors.initials: WJ
authors.lastname: Joo
authors.forename: Cheuk Ho
authors.initials: CH
authors.lastname: Tam
authors.forename: Margaret T
authors.initials: MT
authors.lastname: Fuller
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002455:Cell Division; D019070:Cell Lineage / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D053612:Janus Kinases / Q000378:metabolism; D008297:Male; D008540:Meiosis; D009154:Mutation / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D050791:STAT Transcription Factors / Q000378:metabolism; D015398:Signal Transduction; D013091:Spermatogenesis / Q000235:genetics; D013093:Spermatogonia / Q000166:cytology / Q000378:metabolism; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D013737:Testis / Q000166:cytology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D009687:Nuclear Proteins; D020033:Protein Isoforms; D050791:STAT Transcription Factors; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; C121765:lola protein, Drosophila; D053612:Janus Kinases