title: In vitro effect of hydroxyethyl starch 130/0.42 on canine platelet function.
abstract: OBJECTIVE
  To evaluate the effect of 6% hydroxyethyl starch (HES) solution, with a molecular weight of 130 kDa and a degree of substitution of 0.42, on canine platelet function in vitro.
  
  
  METHODS
  Blood samples from 31 healthy adult dogs.
  
  
  METHODS
  Citrated blood was diluted with saline (0.9% NaCl) solution or HES 130/0.42 in ratios of 1:9 (ie, 1 part saline solution or HES 130/0.42 and 9 parts blood) and 1:3. Platelet plug formation time (closure time [Ct]) was measured with a platelet function analyzer and cartridges coated with collagen and ADP.
  
  
  RESULTS
  Median baseline Ct with citrated blood was 84.0 seconds (interquartile range, 74.5 to 99.5 seconds). Results obtained with 1:9 dilutions with saline solution and HES 130/0.42 were not significantly different from baseline results. The 1:3 dilutions with saline solution and HES 130/0.42 resulted in median Cts of 96.0 seconds (interquartile range, 85.5 to 110.8 seconds) and 112.0 seconds (92.0 to 126.0 seconds), respectively. Results obtained with both 1:3 dilutions were significantly different from baseline results. The Ct obtained with the HES dilution was also significantly different from that of the 1:3 dilution with saline solution.
  
  
  CONCLUSIONS
  Saline solution and HES 130/0.42 in a 1:3 dilution affected canine platelet function by prolonging Cts. The HES 130/0.42 had a significantly greater effect on canine platelets than did saline solution.
authors.affiliation: Clinic of Small Animal Medicine, Ludwig Maximilian University, Veterinaerstrasse 13, 80539 Munich, Germany.
authors.forename: Janine
authors.initials: J
authors.lastname: Classen
authors.forename: Katja N
authors.initials: KN
authors.lastname: Adamik
authors.forename: Karin
authors.initials: K
authors.lastname: Weber
authors.forename: Stephanie
authors.initials: S
authors.lastname: Rubenbauer
authors.forename: Katrin
authors.initials: K
authors.lastname: Hartmann
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D001780:Blood Coagulation Tests / Q000662:veterinary; D001792:Blood Platelets / Q000187:drug effects*; D004285:Dogs; D005260:Female; D006895:Hydroxyethyl Starch Derivatives / Q000008:administration & dosage*; D008297:Male; D010952:Plasma Substitutes / Q000008:administration & dosage*; D010974:Platelet Aggregation / Q000187:drug effects; D010979:Platelet Function Tests / Q000662:veterinary; D012965:Sodium Chloride / Q000008:administration & dosage
chemical_list: D006895:Hydroxyethyl Starch Derivatives; D010952:Plasma Substitutes; D012965:Sodium Chloride