title: ROCK inhibitor Y-27632 increases the cloning efficiency of limbal stem/progenitor cells by improving their adherence and ROS-scavenging capacity.
abstract: Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 has been shown to increase proliferative capacity and even immortalize primary keratinocytes. Here, we demonstrate that rabbit primary limbal epithelial cells (LECs) treated with Y-27632 also exhibited improved colony-forming efficiency by enhancing the expansion of the stem/progenitor cells. Moreover, Y-27632 treatment improved the rapid adherence of limbal stem/progenitor cells in the initial inoculation of primary cells. In addition, Y-27632 treatment elevated the intracellular glutathione level and decreased cellular reactive oxygen species (ROS) accumulation during the expansion of LECs. Therefore, ROCK inhibitor Y-27632 increased the cloning efficiency of rabbit limbal stem/progenitor cells by improving their adherence and ROS scavenging capacity.
authors.affiliation: State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China.
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Zhou
authors.forename: Haoyun
authors.initials: H
authors.lastname: Duan
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.forename: Mingli
authors.initials: M
authors.lastname: Qu
authors.forename: Lingling
authors.initials: L
authors.lastname: Yang
authors.forename: Lixin
authors.initials: L
authors.lastname: Xie
mesh_terms: D000577:Amides / Q000494:pharmacology*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D002999:Clone Cells; D003114:Colony-Forming Units Assay; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016166:Free Radical Scavengers / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D016850:Limbus Corneae / Q000166:cytology*; D011725:Pyridines / Q000494:pharmacology*; D011817:Rabbits; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D000577:Amides; D016166:Free Radical Scavengers; D011725:Pyridines; D017382:Reactive Oxygen Species; C108830:Y 27632; D054460:rho-Associated Kinases; D005978:Glutathione