title: Citricoccus yambaruensis sp. nov., a racemic phenylsuccinate stereospecifically assimilating actinomycete isolated from soil in Okinawa.
abstract: A bacterial strain, designated PS9(T), was isolated from soil in the Ryukyu Archipelago, Japan. The bacterium grew with racemic phenylsuccinate as the sole carbon and energy source. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain PS9(T) was closely related to Citricoccus muralis, C. alkalitolerans and C. nitrophenolicus with sequence similarities of 97.5, 97.8, and 98.3%, respectively, suggesting that the strain belonged to the genus Citricoccus. Strain PS9(T) was a Gram-positive, non-motile, circular-shaped and aerobic bacterium. The major respiratory quinone was MK-8 (H(2)) and the predominant cellular fatty acid was C(15:0) anteiso, C(17:0) anteiso, and C(15:0) iso. The G+C content was 72.4 mol%. Based on the phylogenetic and phenotypic traits, it was concluded that the organism represents a new species in the genus Citricoccus, with the name Citricoccus yambaruensis. The type strain is PS9(T) (=NBRC102121(T) = DSM18783(T)).
authors.affiliation: Tropical Biosphere Research Center, University of the Ryukyus, Nishihara, Okinawa, Japan. tmatsui@comb.u-ryukyu.ac.jp
authors.forename: Toru
authors.initials: T
authors.lastname: Matsui
authors.forename: Hisashi
authors.initials: H
authors.lastname: Semba
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hanada
mesh_terms: D005227:Fatty Acids / Q000032:analysis; D005798:Genes, Bacterial; D008835:Micrococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010641:Phenotype; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013045:Species Specificity; D013237:Stereoisomerism; D013386:Succinates / Q000032:analysis*
chemical_list: D005227:Fatty Acids; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013386:Succinates; C017625:2-phenylsuccinate