title: Polymeric nanoparticles to control the differentiation of neural stem cells in the subventricular zone of the brain.
abstract: Herein, we report the use of retinoic acid-loaded polymeric nanoparticles as a potent tool to induce the neuronal differentiation of subventricular zone neural stem cells. The intracellular delivery of retinoic acid by the nanoparticles activated nuclear retinoic acid receptors, decreased stemness, and increased proneurogenic gene expression. Importantly, this work reports for the first time a nanoparticle formulation able to modulate in vivo the subventricular zone neurogenic niche. The work further compares the dynamics of initial stages of differentiation between SVZ cells treated with retinoic acid-loaded polymeric nanoparticles and solubilized retinoic acid. The nanoparticle formulation developed here may ultimately offer new perspectives to treat neurodegenerative diseases.
authors.affiliation: CNC - Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: Tiago
authors.initials: T
authors.lastname: Santos
authors.forename: Raquel
authors.initials: R
authors.lastname: Ferreira
authors.forename: João
authors.initials: J
authors.lastname: Maia
authors.forename: Fabienne
authors.initials: F
authors.lastname: Agasse
authors.forename: Sara
authors.initials: S
authors.lastname: Xapelli
authors.forename: Luísa
authors.initials: L
authors.lastname: Cortes
authors.forename: José
authors.initials: J
authors.lastname: Bragança
authors.forename: João O
authors.initials: JO
authors.lastname: Malva
authors.forename: Lino
authors.initials: L
authors.lastname: Ferreira
authors.forename: Liliana
authors.initials: L
authors.lastname: Bernardino
mesh_terms: D000818:Animals; D001369:Axons / Q000187:drug effects / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects; D020547:Lateral Ventricles / Q000166:cytology*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011094:Polyethyleneimine / Q000737:chemistry*; D018168:Receptors, Retinoic Acid / Q000378:metabolism; D014212:Tretinoin / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D004337:Drug Carriers; D009419:Nerve Tissue Proteins; D011094:Polyethyleneimine; D018168:Receptors, Retinoic Acid; D014212:Tretinoin; C499317:Ascl1 protein, mouse; C486336:Neurog1 protein, mouse