title: Notch1-mediated signaling regulates proliferation of porcine satellite cells (PSCs).
abstract: Notch signaling is an evolutionarily conserved cell-cell communication mechanism involved in the regulation of cell proliferation, differentiation and fate decisions of mammalian cells. In the present study, we investigated the possible requirement for Notch signaling in the proliferation and differentiation of porcine satellite cells. We show that Notch1, 2 and 3 are expressed in cultured porcine satellite cells. Knock-down of NOTCH1, but not NOTCH2 and NOTCH3, decreases the proliferation of porcine satellite cells. In contrast, enhancement of NOTCH1 expression via treatment of porcine satellite cells with recombinant NF-κB increases the proliferation of porcine satellite cells. The alteration of porcine satellite cell proliferation is associated with significant changes in the expression of cell cycle related genes (cyclin B1, D1, D2, E1 and p21), myogenic regulatory factors (MyoD and myogenin) and the Notch effector Hes5. In addition, alteration of Notch1 expression in porcine satellite cells causes changes in the expression of GSK3β-3. Taken together, these findings suggest that of the four notch-related genes, Notch1is likely to be required for regulating the proliferation and therefore the maintenance of porcine satellite cells in vivo, and do so through activation of the Notch effector gene Hes5.
authors.affiliation: College of Animal Science/Guangdong Provincial Key Lab of Agro-animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou, Guangdong, 510642, People's Republic of China.
authors.forename: Lili
authors.initials: L
authors.lastname: Qin
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.forename: Zhenfang
authors.initials: Z
authors.lastname: Wu
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Li
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.forename: Qiaoming
authors.initials: Q
authors.lastname: Long
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D038362:Glycogen Synthase Kinase 3 / Q000235:genetics / Q000378:metabolism; D000071679:Glycogen Synthase Kinase 3 beta; D006801:Humans; D017570:MyoD Protein / Q000378:metabolism; D018008:Myogenin / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D051881:Receptor, Notch1 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D012097:Repressor Proteins / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D015398:Signal Transduction; D034421:Sus scrofa; D051153:Wnt Proteins / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D017570:MyoD Protein; D018008:Myogenin; D016328:NF-kappa B; D034741:RNA, Small Interfering; D051881:Receptor, Notch1; D011994:Recombinant Proteins; D012097:Repressor Proteins; D051153:Wnt Proteins; C000605506:GSK3B protein, human; D000071679:Glycogen Synthase Kinase 3 beta; D038362:Glycogen Synthase Kinase 3