title: Phylogenetic relationships of the glycoprotein gene of bovine ephemeral fever virus isolated from mainland China, Taiwan, Japan, Turkey, Israel and Australia.
abstract: BACKGROUND
  The glycoprotein (G) gene sequences of bovine ephemeral fever virus (BEFV) strains derived from mainland China have not been compared with those of the isolates from other countries or areas. Therefore, the G genes of four BEFV isolates obtained from mainland China were amplified and sequenced. A phylogenetic tree was constructed in order to compare and analyze the genetic relationships of the BEFV isolates derived from mainland China and different countries and areas.
  
  
  RESULTS
  The complete BEFV G gene was successfully amplified and sequenced from four isolates that originated from mainland China. A total of fifty-one BEFV strains were analyzed based on the G gene sequence and were found to be highly conserved. A phylogenetic tree showed that the isolates were grouped into three distinct lineages depending on their source of origin. The antigenic sites of G1, G2 and G3 are conserved among the isolates, except for several substitutions in a few strains.
  
  
  CONCLUSIONS
  The phylogenetic relationships of the BEFV isolates that originated from mainland China, Taiwan, Japan, Turkey, Israel and Australia were closely related to their source of origin, while the antigenic sites G1, G2 and G3 are conserved among the BEFV isolates used in this work.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No, 1 Xujiaping, Yanchangbao, Lanzhou, 730046, China. zfycaas@126.com
authors.forename: Fuying
authors.initials: F
authors.lastname: Zheng
authors.forename: Changqing
authors.initials: C
authors.lastname: Qiu
mesh_terms: D000818:Animals; D001208:Asia; D001315:Australia; D002417:Cattle; D017124:Conserved Sequence; D004810:Ephemeral Fever / Q000821:virology*; D018115:Ephemeral Fever Virus, Bovine / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D000939:Epitopes / Q000235:genetics; D014644:Genetic Variation; D006023:Glycoproteins / Q000235:genetics*; D008877:Middle East; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D000939:Epitopes; D006023:Glycoproteins; D012367:RNA, Viral; D014764:Viral Proteins; C077307:BEFV G protein, Bovine ephemeral fever virus