title: Structural analysis of a heteropolysaccharide from Saccharina japonica by electrospray mass spectrometry in tandem with collision-induced dissociation tandem mass spectrometry (ESI-CID-MS/MS).
abstract: A fucoidan extracted from Saccharina japonica was fractionated by anion exchange chromatography. The most complex fraction F0.5 was degraded by dilute sulphuric acid and then separated by use of an activated carbon column. Fraction Y1 was fractionated by anion exchange and gel filtration chromatography while Fraction Y2 was fractionated by gel filtration chromatography. The fractions were determined by ESI-MS and analyzed by ESI-CID-MS/MS. It was concluded that F0.5 had a backbone of alternating 4-linked GlcA and 2-linked Man with the first Man residue from the nonreducing end accidentally sulfated at C6. In addition, F0.5 had a 3-linked glucuronan, in accordance with a previous report by NMR. Some other structural characteristics included GlcA 1→3 Man 1→4 GlcA, Man 1→3 GlcA 1→4 GlcA, Fuc 1→4 GlcA and Fuc 1→3 Fuc. Finally, it was shown that fucose was sulfated at C2 or C4 while galactose was sulfated at C2, C4 or C6.
authors.affiliation: Institute of Oceanology, Chinese Academy of Science, Qingdao 266071, China.|University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Jin
authors.affiliation: Institute of Oceanology, Chinese Academy of Science, Qingdao 266071, China.|Nantong Marine Science and Technology R & D Center, IOCAS, Jiangsu 226006, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Medicine and Pharmaceutics, Ocean University of China, Qingdao 266003, China.
authors.forename: Sumei
authors.initials: S
authors.lastname: Ren
authors.affiliation: College of Medicine and Pharmaceutics, Ocean University of China, Qingdao 266003, China.
authors.forename: Ni
authors.initials: N
authors.lastname: Song
authors.affiliation: Institute of Oceanology, Chinese Academy of Science, Qingdao 266071, China.
authors.forename: Quanbin
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D000459:Phaeophyceae / Q000737:chemistry* / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D011134:Polysaccharides