title: Cell viability and angiogenic potential of a bioartificial adipose substitute.
abstract: An implantable scaffold pre-seeded with cells needs to remain viable and encourage rapid angiogenesis in order to replace injured tissues, especially for tissue defect repairs. We created a bioartificial adipose graft composed of an electrospun 3D nanofibrous scaffold and fat tissue excised from New Zealand white rabbits. Cell viability and angiogenesis potential of the bioartificial substitute were examined during four weeks of culture in Dulbecco's Modified Eagle Medium by immunohistochemical staining with LIVE/DEAD® cell kit and PECAM-1 antibody, respectively. In addition, a Matrigel® assay was performed to examine the possibility of blood vessels sprouting from the bioartificial graft. Our results showed that cells within the graft were viable and vascular tubes were present at week 4, while cells in a fat tissue block were dead in vitro. In addition, capillaries were observed sprouting from the graft into the Matrigel, demonstrating its angiogenic potential. We expect that improved cell viability and angiogenesis in the bioartificial substitute, compared to intact autologous graft, could potentially contribute to its survival following implantation.
authors.affiliation: Department of General Surgery, National University Healthcare System, Singapore.
authors.forename: Anitha
authors.initials: A
authors.lastname: Panneerselvan
authors.affiliation: NUS Graduate School for Integrative Sciences and Engineering (NGS), National University of Singapore, Singapore.
authors.forename: Luong T H
authors.initials: LT
authors.lastname: Nguyen
authors.affiliation: College of Chemistry & Chemical Engineering and Biotechnology, Donghua University, Shanghai, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Su
authors.affiliation: Biomers Pte Ltd, Singapore.
authors.forename: Wee Eong
authors.initials: WE
authors.lastname: Teo
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University, Singapore.
authors.forename: Susan
authors.initials: S
authors.lastname: Liao
authors.affiliation: Department of Mechanical Engineering, National University of Singapore, Singapore.|King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia.
authors.forename: Seeram
authors.initials: S
authors.lastname: Ramakrishna
authors.affiliation: Department of General Surgery, National University Healthcare System, Singapore.
authors.forename: Ching Wan
authors.initials: CW
authors.lastname: Chan
mesh_terms: D000273:Adipose Tissue / Q000098:blood supply / Q000166:cytology* / Q000637:transplantation / Q000648:ultrastructure; D000818:Animals; D001187:Artificial Organs; D002470:Cell Survival; D005109:Extracellular Matrix / Q000648:ultrastructure; D057139:Nanofibers / Q000648:ultrastructure; D018919:Neovascularization, Physiologic; D011817:Rabbits; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry