title: Identifying genetic susceptibility to sensitization to cephalosporins in health care workers.
abstract: Exposure to cephalosporins could cause occupational allergic diseases in health care workers (HCWs). We evaluated the prevalence of serum specific IgE and IgG antibodies to cephalosporin-human serum albumin (HSA) conjugate and to identify potential genetic risk factors associated with sensitization to cephalosporins in exposed HCWs. The study population consisted of 153 HCWs who had been exposed to antibiotics in a single university hospital and 86 unexposed healthy controls. A questionnaire survey of work-related symptoms (WRS) was administered. A skin-prick test (SPT) was performed, and serum-specific IgE and IgG antibodies to 3 commonly prescribed cephalosporins were measured by ELISA. Four single-nucleotide polymorphisms of the candidate genes related to IgE sensitization were genotyped. The prevalence of WRS to cephalosporins was 2.6%. The prevalence rates of serum-specific IgE and IgG antibodies to cephalosporins were 20.3% and 14.7%, respectively. The FcεR1β-109T > C polymorphism was significantly associated with IgE sensitization to cephalosporins in HCWs (P = 0.036, OR = 3.553; CI, 1.324-9.532). The in vitro functional assay demonstrated that the T allele of FcεR1β-109T had greater promoter activity than did the C allele (P < 0.001). The FcεR1β-109T > C polymorphism may be a potential genetic risk factor for increased IgE sensitization to cephalosporins.
authors.affiliation: Department of Allergy & Clinical Immunology, Ajou University School of Medicine, Suwon, Korea.
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Nam
authors.forename: Jeong-Eun
authors.initials: JE
authors.lastname: Kim
authors.forename: Seung-Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Jin
authors.forename: Eui-Kyung
authors.initials: EK
authors.lastname: Hwang
authors.forename: Yoo-Seob
authors.initials: YS
authors.lastname: Shin
authors.forename: Young-Min
authors.initials: YM
authors.lastname: Ye
authors.forename: Hae-Sim
authors.initials: HS
authors.lastname: Park
mesh_terms: D000328:Adult; D000483:Alleles; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000276:immunology*; D002511:Cephalosporins / Q000032:analysis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020022:Genetic Predisposition to Disease; D006282:Health Personnel; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis* / Q000453:epidemiology; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D009784:Occupational Diseases / Q000139:chemically induced* / Q000453:epidemiology; D016273:Occupational Exposure; D016017:Odds Ratio; D017455:Receptors, IgE / Q000235:genetics; D012882:Skin Tests; D011795:Surveys and Questionnaires; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D007074:Immunoglobulin G; D017455:Receptors, IgE; D007073:Immunoglobulin E