title: Xanthone derivatives as potential inhibitors of miRNA processing by human Dicer: targeting secondary structures of pre-miRNA by small molecules.
abstract: In recent years, various biological processes have been found to be regulated by miRNA-mediated gene silencing. A small molecule that modulate the miRNA pathway will provide the biological tool for elucidating mechanisms of miRNA-mediated gene regulation, and can be the drug lead for miRNA related diseases. In this study, we demonstrated that an aminoalkoxy-substituted thioxanthone derivative interferes Dicer-mediated processing of pre-miRNA. Information about the interaction between these xanthone derivatives and pre-miRNAs will enable us to design and develop new small molecule-based inhibitors for miRNA pathway.
authors.affiliation: Department of Regulatory Bioorganic Chemistry, The Institute of Scientific and Industrial Research, Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan.
authors.forename: Asako
authors.initials: A
authors.lastname: Murata
authors.forename: Takeo
authors.initials: T
authors.lastname: Fukuzumi
authors.forename: Shiori
authors.initials: S
authors.lastname: Umemoto
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakatani
mesh_terms: D053487:DEAD-box RNA Helicases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D034622:RNA Interference; D043244:Ribonuclease III / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013892:Thioxanthenes / Q000737:chemistry / Q000378:metabolism; D044004:Xanthones / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D035683:MicroRNAs; D013892:Thioxanthenes; D044004:Xanthones; C009689:xanthone; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases; C484911:thioxanthone