title: Structural and binding properties of two paralogous fatty acid binding proteins of Taenia solium metacestode.
abstract: BACKGROUND
  Fatty acid (FA) binding proteins (FABPs) of helminths are implicated in acquisition and utilization of host-derived hydrophobic substances, as well as in signaling and cellular interactions. We previously demonstrated that secretory hydrophobic ligand binding proteins (HLBPs) of Taenia solium metacestode (TsM), a causative agent of neurocysticercosis (NC), shuttle FAs in the surrounding host tissues and inwardly transport the FAs across the parasite syncytial membrane. However, the protein molecules responsible for the intracellular trafficking and assimilation of FAs have remained elusive.
  
  
  RESULTS
  We isolated two novel TsMFABP genes (TsMFABP1 and TsMFABP2), which encoded 133- and 136-amino acid polypeptides with predicted molecular masses of 14.3 and 14.8 kDa, respectively. They shared 45% sequence identity with each other and 15-95% with other related-members. Homology modeling demonstrated a characteristic β-barrel composed of 10 anti-parallel β-strands and two α-helices. TsMFABP2 harbored two additional loops between β-strands two and three, and β-strands six and seven, respectively. TsMFABP1 was secreted into cyst fluid and surrounding environments, whereas TsMFABP2 was intracellularly confined. Partially purified native proteins migrated to 15 kDa with different isoelectric points of 9.2 (TsMFABP1) and 8.4 (TsMFABP2). Both native and recombinant proteins bound to 11-([5-dimethylaminonaphthalene-1-sulfonyl]amino)undecannoic acid, dansyl-DL-α-amino-caprylic acid, cis-parinaric acid and retinol, which were competitively inhibited by oleic acid. TsMFABP1 exhibited high affinity toward FA analogs. TsMFABPs showed weak binding activity to retinol, but TsMFABP2 showed relatively high affinity. Isolation of two distinct genes from an individual genome strongly suggested their paralogous nature. Abundant expression of TsMFABP1 and TsMFABP2 in the canal region of worm matched well with the histological distributions of lipids and retinol.
  
  
  CONCLUSIONS
  The divergent biochemical properties, physiological roles and cellular distributions of the TsMFABPs might be one of the critical mechanisms compensating for inadequate de novo FA synthesis. These proteins might exert harmonized or independent roles on lipid assimilation and intracellular signaling. The specialized distribution of retinol in the canal region further implies that cells in this region might differentiate into diverse cell types during metamorphosis into an adult worm. Identification of bioactive systems pertinent to parasitic homeostasis may provide a valuable target for function-related drug design.
authors.affiliation: Department of Molecular Parasitology, Sungkyunkwan University School of Medicine and Center for Molecular Medicine, Samsung Biomedical Research Center, Suwon, Korea.
authors.forename: Seon-Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Young-An
authors.initials: YA
authors.lastname: Bae
authors.forename: Hyun-Jong
authors.initials: HJ
authors.lastname: Yang
authors.forename: Joo-Ho
authors.initials: JH
authors.lastname: Shin
authors.forename: Sylvia Paz
authors.initials: SP
authors.lastname: Diaz-Camacho
authors.forename: Yukifumi
authors.initials: Y
authors.lastname: Nawa
authors.forename: Insug
authors.initials: I
authors.lastname: Kang
authors.forename: Yoon
authors.initials: Y
authors.lastname: Kong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D050556:Fatty Acid-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010802:Phylogeny; D011485:Protein Binding; D011487:Protein Conformation; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D041201:Taenia solium / Q000235:genetics*
chemical_list: D018509:DNA, Helminth; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids