title: Role of vascular endothelial growth factor and angiopoietin 1 in renal injury in hemolytic uremic syndrome.
abstract: OBJECTIVE
  The recovery process from renal injury in hemolytic uremic syndrome (HUS) remains obscure. In order to clarify the role of vascular endothelial growth factor (VEGF) and angiopoietin 1 (Ang-1) in the renal recovery from HUS, we produced a model of mild HUS and examined the renal recovery process.
  
  
  METHODS
  We investigated three groups of mice. Group 1 consisted of mice that received an injection of Shiga toxin 2 (Stx2) and lipopolysaccharide (LPS); group 2 consisted of mice that received an injection of low dose of Stx2 and LPS, and group 3 consisted of control mice.
  
  
  RESULTS
  Serum Cr levels in group 1 were greater than those in group 2, and all mice in group 1 died, whereas all mice in group 2 remained alive. Endothelial injury at 24 h in group 1 was higher than in group 2. Electron-microscopic findings demonstrated that the endothelial cells formed immature capillary-like lumina from 7 to 28 days with increases in the expression of CD31-positive cells. Glomerular VEGF expression decreased at 72 h in group 1, but gradually increased in group 2. Glomerular Ang-1 expression peaked from 72 h to 28 days. Ang-1 expression was frequently found in the endothelial cell region of vesicle walls simultaneous with increased CD31-positive staining.
  
  
  CONCLUSIONS
  Our findings suggest that VEGF and Ang-1 play important roles in the recovery process, particularly in the regeneration of endothelial injury.
authors.affiliation: Department of Pediatrics, Fukushima Medical University School of Medicine, Fukushima, Japan.
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Ohara
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Kawasaki
authors.forename: Yusaku
authors.initials: Y
authors.lastname: Abe
authors.forename: Masahiro
authors.initials: M
authors.lastname: Watanabe
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ono
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Suyama
authors.forename: Koichi
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Takashi
authors.initials: T
authors.lastname: Honda
authors.forename: Junzo
authors.initials: J
authors.lastname: Suzuki
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Hosoya
mesh_terms: D000199:Actins / Q000378:metabolism; D058186:Acute Kidney Injury / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D042683:Angiopoietin-1 / Q000378:metabolism*; D000818:Animals; D003404:Creatinine / Q000097:blood; D004727:Endothelium / Q000502:physiology*; D006463:Hemolytic-Uremic Syndrome / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D007678:Kidney Glomerulus / Q000378:metabolism / Q000473:pathology; D008070:Lipopolysaccharides; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D020127:Recovery of Function; D012038:Regeneration; D022641:Shiga Toxin 2; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D000199:Actins; D042683:Angiopoietin-1; D008070:Lipopolysaccharides; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D022641:Shiga Toxin 2; D042461:Vascular Endothelial Growth Factor A; C544013:alpha-smooth muscle actin, mouse; D003404:Creatinine