title: Reduction of helical content by insertion of a disulfide bond leads to an antimicrobial peptide with decreased hemolytic activity.
abstract: CAPs off to the S-S bond: Application of a strategy involving the introduction of intramolecular disulfide bonds to amphipathic cationic antimicrobial peptides (CAPs) led to decreased α-helicity and hydrophobicity. The mutant peptides were observed to have significantly increased (250-fold) minimum hemolytic concentrations while maintaining MIC values against E. coli, affording more therapeutically selective CAPs.
authors.affiliation: Department of Chemistry and Education, Seoul National University, Seoul, 151-742, Korea.
authors.forename: Heeyong
authors.initials: H
authors.lastname: Hwang
authors.forename: Soonsil
authors.initials: S
authors.lastname: Hyun
authors.forename: Yangsoo
authors.initials: Y
authors.lastname: Kim
authors.forename: Jaehoon
authors.initials: J
authors.lastname: Yu
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D004220:Disulfides / Q000737:chemistry*; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects / Q000382:microbiology; D004926:Escherichia coli / Q000187:drug effects; D004927:Escherichia coli Infections / Q000188:drug therapy; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D013203:Staphylococcal Infections / Q000188:drug therapy; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D004220:Disulfides