title: Adeno-associated virus Rep78 restricts adenovirus E1B55K-mediated p53 nuclear exportation.
abstract: Inactivation of p53 is needed during adenovirus type 5 DNA replication. E1B55K, an adenovirus early protein, has been reported to interact with p53 and inhibit p53 transactivation. Previous studies have shown that adeno-associated virus (AAV) type 2 could reduce the transforming potential of adenovirus by rescuing p53 from adenovirus-mediated degradation, but the details are not clear yet. We detected the Rep78-p53 interaction by co-immunoprecipitation assay. The co-localization assay revealed that Rep78 inhibits E1B55K-mediated p53 nuclear exportation. However, Rep78 did not detectably influence p53 stability and could not relieve the transcriptional inactivation of p53, as E1B55K could not be replaced from the p53-E1B55K complex by Rep78. Our results reveal a new possible mechanism that AAV-2 Rep78 inhibits adenovirus 5 by relocalizing p53 in the nucleus, which may shed some light on the regulatory mechanism of AAV-2 on its helper virus, adenovirus.
authors.affiliation: Institute of Genetics, School of Life Sciences,  Fudan University, Shanghai 200433,  China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wang
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Li
authors.forename: Ran
authors.initials: R
authors.lastname: Wang
authors.forename: Jinglun
authors.initials: J
authors.lastname: Xue
authors.forename: Jinzhong
authors.initials: J
authors.lastname: Chen
mesh_terms: D021581:Active Transport, Cell Nucleus; D045744:Cell Line, Tumor; D000229:Dependovirus / Q000502:physiology*; D006801:Humans; D012516:Osteosarcoma / Q000378:metabolism* / Q000821:virology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D014764:Viral Proteins
chemical_list: C553319:E1B55K protein, adenovirus; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D014764:Viral Proteins