title: Intestinal perfusion indicates high reliance on paracellular nutrient absorption in an insectivorous bat Tadarida brasiliensis.
abstract: Flying vertebrates have been hypothesized to have a high capacity for paracellular absorption of nutrients. This could be due to high permeability of the intestines to nutrient-sized molecules (i.e., in the size range of amino acids and glucose, MW 75-180 Da). We performed intestinal luminal perfusions of an insectivorous bat, Tadarida brasiliensis. Using radio-labeled molecules, we measured the uptake of two nutrients absorbed by paracellular and transporter-mediated mechanisms (L-proline, MW 115 Da, and D-glucose, MW 180 Da) and two carbohydrates that have no mediated transport (L-arabinose, MW 150 Da, and lactulose, MW 342 Da). Absorption of lactulose (0.61±0.06 nmol min(-1) cm(-1)) was significantly lower than that of the smaller arabinose (1.09±0.04 nmol min(-1) cm(-1)). Glucose absorption was significantly lower than that of proline at both nutrient concentrations (10mM and 75 mM). Using the absorption of arabinose to estimate the portion of proline absorption that is paracellular, we calculated that 25.1±3.0% to 66.2±7.8% of proline absorption is not transporter-mediated (varying proline from 1 mM to 75 mM). These results confirm our predictions that 1) paracellular absorption is molecule size selective, 2) absorption of proline would be greater than glucose absorption in an insectivore, and 3) paracellular absorption represents a large fraction of total nutrient absorption in bats.
authors.affiliation: Department of Forest and Wildlife Ecology, University of Wisconsin-Madison, Madison, WI 53706, USA. eprice2@wisc.edu
authors.forename: Edwin R
authors.initials: ER
authors.lastname: Price
authors.forename: Antonio
authors.initials: A
authors.lastname: Brun
authors.forename: Verónica
authors.initials: V
authors.lastname: Fasulo
authors.forename: William H
authors.initials: WH
authors.lastname: Karasov
authors.forename: Enrique
authors.initials: E
authors.lastname: Caviedes-Vidal
mesh_terms: D000818:Animals; D001089:Arabinose / Q000378:metabolism; D001692:Biological Transport / Q000502:physiology*; D002685:Chiroptera / Q000378:metabolism / Q000502:physiology; D007312:Eulipotyphla / Q000378:metabolism / Q000502:physiology; D005947:Glucose / Q000378:metabolism; D007408:Intestinal Absorption / Q000502:physiology*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000502:physiology; D007792:Lactulose / Q000378:metabolism; D010477:Perfusion; D010539:Permeability
chemical_list: D007792:Lactulose; D001089:Arabinose; D005947:Glucose