title: Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly.
abstract: Numerous nanoscale devices and materials have been fabricated in recent years using a variety of biological scaffolds. However, the interfacing of these devices and materials into existing circuits and ordered arrays has proved problematic. Here, we describe a simple solution to this problem using self-assembly of the peptide coiled-coil heterodimer ACID:BASE to immobilize M13 bacteriophage particles to specific locations on a patterned gold surface. Surface plasmon resonance demonstrated that free ACID peptides will assemble onto a surface derivatized with BASE. We then displayed the ACID peptide on the pIX coat protein of M13 and showed that these phage particles permit formation of the coiled-coil resulting in specific surface attachment. The ACID:immobilized BASE affinities appear to be similar for free peptide and phage-displayed ACID. Finally, we fabricated two gold electrodes, separated by a 200 nm gap, coated one of them with BASE and showed that this allows localization of the M13:ACID onto the functionalized electrode.
authors.affiliation: Astbury Centre for Structural Molecular Biology, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK.
authors.forename: Simon J
authors.initials: SJ
authors.lastname: White
authors.affiliation: School of Electronic and Electrical Engineering, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK.|Department of Electronics, University of York, Heslington, York, Y010 5DD, UK.
authors.forename: Steven
authors.initials: S
authors.lastname: Johnson
authors.affiliation: School of Electronic and Electrical Engineering, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK.
authors.forename: Michal
authors.initials: M
authors.lastname: Szymonik
authors.affiliation: Astbury Centre for Structural Molecular Biology, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Wardingley
authors.affiliation: Astbury Centre for Structural Molecular Biology, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK.
authors.forename: Douglas
authors.initials: D
authors.lastname: Pye
authors.affiliation: School of Electronic and Electrical Engineering, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK.
authors.forename: A Giles
authors.initials: AG
authors.lastname: Davies
authors.affiliation: School of Electronic and Electrical Engineering, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK.
authors.forename: Christoph
authors.initials: C
authors.lastname: Wälti
authors.affiliation: Astbury Centre for Structural Molecular Biology, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK.
authors.forename: Peter G
authors.initials: PG
authors.lastname: Stockley
mesh_terms: D000327:Adsorption; D017104:Bacteriophage M13 / Q000737:chemistry*; D003217:Conductometry / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D014771:Virion / Q000737:chemistry*
chemical_list: D010455:Peptides; D006046:Gold