title: [The role of CXCR3 and its ligand I-TAC in the pathogenesis of immune thrombocytopenic purpura].
abstract: OBJECTIVE
  To investigate the roles of the chemokine receptor CXCR3 and its ligand I-TAC in the pathogenesis of immune thrombocytopenic purpura (ITP).
  
  
  METHODS
  A total of 48 ITP patients were enrolled in this study: 30 with newly diagnosed or relapse ITP and 18 in remission after treatment, and 24 healthy volunteers were as controls. IFNγ and I-TAC in plasma were detected by ELISA. The mRNA expression of CXCR3 in the peripheral blood mononuclear cells (PBMNCs) was determined by quantitative RT-PCR.
  
  
  RESULTS
  The IFNγ level in the plasma of ITP patients before the treatment was obviously increased than those in the remission group and controls [(71.45 ± 17.62) ng/L vs (36.94 ± 14.86) ng/L and (25.28 ± 12.85) ng/L, all P < 0.05] and those in the remission group was higher than in the controls (P < 0.05). In contrast, there were no statistic differences of the levels of I-TAC among the three groups [(455.56 ± 144.70) ng/L, (488.24 ± 164.70) ng/L and (382.97 ± 167.43) ng/L, P > 0.05]. Both ITP patients before the treatment and remission groups expressed more CXCR3 mRNA [6.76 (3.03, 37.00), 1.76 (0.45, 14.18) vs 0.12 (0.04, 0.28), P < 0.05]. After effective therapy, CXCR3 mRNA expression decreased, while it was still higher than that in the controls.
  
  
  CONCLUSIONS
  Our data demonstrate that Th1 cytokine (IFNγ) dominance is reflected in ITP. Simultaneously, the CXCR3(+) cell may play a role in cell-mediated immunity through chemotaxis in ITP.
authors.affiliation: Department of Hematology, Qinghai Provincial People's Hospital, Xining, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.forename: Jian-ming
authors.initials: JM
authors.lastname: Feng
authors.forename: Wen-qian
authors.initials: WQ
authors.lastname: Li
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Li
authors.forename: Shao-bin
authors.initials: SB
authors.lastname: Chen
authors.forename: Guo-xiong
authors.initials: GX
authors.lastname: Han
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D054371:Chemokine CXCL11 / Q000097:blood*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000097:blood / Q000378:metabolism*; D054367:Receptors, CXCR3 / Q000378:metabolism*; D055815:Young Adult
chemical_list: C515202:CXCL11 protein, human; C515187:CXCR3 protein, human; D054371:Chemokine CXCL11; D054367:Receptors, CXCR3; D007371:Interferon-gamma