title: Identification of genetic suppressors of the Sin3A knockdown wing phenotype.
abstract: The role of the Sin3A transcriptional corepressor in regulating the cell cycle is established in various metazoans. Little is known, however, about the signaling pathways that trigger or are triggered by Sin3A function. To discover genes that work in similar or opposing pathways to Sin3A during development, we have performed an unbiased screen of deficiencies of the Drosophila third chromosome. Additionally, we have performed a targeted loss of function screen to identify cell cycle genes that genetically interact with Sin3A. We have identified genes that encode proteins involved in regulation of gene expression, signaling pathways and cell cycle that can suppress the curved wing phenotype caused by the knockdown of Sin3A. These data indicate that Sin3A function is quite diverse and impacts a wide variety of cellular processes.
authors.affiliation: Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, United States of America.
authors.forename: Aishwarya
authors.initials: A
authors.lastname: Swaminathan
authors.forename: Valerie L
authors.initials: VL
authors.lastname: Barnes
authors.forename: Stephanie
authors.initials: S
authors.lastname: Fox
authors.forename: Sarah
authors.initials: S
authors.lastname: Gammouh
authors.forename: Lori A
authors.initials: LA
authors.lastname: Pile
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D059006:Chromosomes, Insect; D056850:Cyclin-Dependent Kinase 8 / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D004843:Epistasis, Genetic; D005260:Female; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D008297:Male; D010641:Phenotype; D034622:RNA Interference; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D056264:Sin3 Histone Deacetylase and Corepressor Complex; D014158:Transcription, Genetic; D014921:Wings, Animal / Q000033:anatomy & histology / Q000254:growth & development* / Q000473:pathology
chemical_list: D018797:Cell Cycle Proteins; D012097:Repressor Proteins; C099683:SIN3A transcription factor; D056850:Cyclin-Dependent Kinase 8; D056264:Sin3 Histone Deacetylase and Corepressor Complex