title: Cardioprotective molecules are enriched in beating cardiomyocytes derived from human embryonic stem cells.
abstract: BACKGROUND
  Cardiomyocytes derived from human embryonic stem cells (hESC-CMs) have attracted attention because of their cardiac regenerative potential in vivo. Differentiated CMs can be distinguished into two different phenotypic populations: beating and non-beating CMs. A thorough understanding of the different molecular conditions of beating and non-beating CMs would provide valuable information for other potential applications and cell therapy.
  
  
  METHODS
  In this study, we generated a comparative protein profiles using proteomic analysis and western blotting, to compare the specific protein expression patterns of beating and non-beating hESC-CMs.
  
  
  RESULTS
  Abundantly 72 upregulated proteins are involved in different biological processes such as stimulus response, cellular catabolism and cell motility. Among these proteins, such as HSPs and other antioxidant molecules, are known to be proteins that potentially play an important role in cardioprotection through the enhancement of cell survival in hypoxic and ischemic conditions present in the injured heart.
  
  
  CONCLUSIONS
  As a first step toward understanding the different molecular conditions of beating and non-beating hESC-CMs, we sought to study their differential expression patterns and discuss their relevance to in vivo functioning in cardiac injury repair. Thus, the results of this study could provide further evidence supporting a cardiac regenerative approach using an optimized cell source derived from hESCs.
authors.affiliation: Department of Oral Pharmacology, School of Dentistry and Institute of Oral Bioscience, BK21 Project, Chonbuk National University, Jeonju, Republic of Korea.
authors.forename: Jung-Il
authors.initials: JI
authors.lastname: Chae
authors.forename: Jumi
authors.initials: J
authors.lastname: Kim
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Son
authors.forename: Young-Joo
authors.initials: YJ
authors.lastname: Jeon
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Kim
authors.forename: Hye Eun
authors.initials: HE
authors.lastname: Kim
authors.forename: Min Ji
authors.initials: MJ
authors.lastname: Lee
authors.forename: Joohyun
authors.initials: J
authors.lastname: Ryu
authors.forename: Byoung Chul
authors.initials: BC
authors.lastname: Park
authors.forename: Dong-Seok
authors.initials: DS
authors.lastname: Lee
authors.forename: Kang Seok
authors.initials: KS
authors.lastname: Seo
authors.forename: Hak Kyo
authors.initials: HK
authors.lastname: Lee
authors.forename: Nag-Jin
authors.initials: NJ
authors.lastname: Choi
authors.forename: Yong-Kook
authors.initials: YK
authors.lastname: Kang
authors.forename: Hyung Min
authors.initials: HM
authors.lastname: Chung
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D053595:Embryonic Stem Cells / Q000502:physiology*; D053263:Gene Regulatory Networks / Q000502:physiology; D006801:Humans; D051379:Mice; D009200:Myocardial Contraction / Q000502:physiology*; D032383:Myocytes, Cardiac / Q000502:physiology*