title: Connexin45 modulates the proliferation of transit-amplifying precursor cells in the mouse subventricular zone.
abstract: Connexins have been implicated in the regulation of precursor cell migration and proliferation during embryonic development of the mammalian brain. However, their function in postnatal neurogenesis is unclear. Here we demonstrate that connexin (Cx) 45 is expressed in transit-amplifying cells and neuroblasts in the postnatal subventricular zone (SVZ) and modulated the proliferation of SVZ-derived precursor cells in vivo. Thus, overexpression of Cx45 by retroviral injections increased the proliferation of Mash-1-positive transit-amplifying precursor cells in the SVZ. Conversely, conditional deletion of Cx45 in precursor cells decreased proliferation. Finally, we established that Cx45 positively influences cell cycle reentry via ATP signaling that involves intracellular calcium stores and ERK1/2 signaling.
authors.affiliation: Department of Clinical Neurobiology, University Hospital and German Cancer Research Center, DKFZ, Heidelberg, 69120 Heidelberg, Germany.
authors.forename: Konstantin
authors.initials: K
authors.lastname: Khodosevich
authors.forename: Annalisa
authors.initials: A
authors.lastname: Zuccotti
authors.forename: Maria M
authors.initials: MM
authors.lastname: Kreuzberg
authors.forename: Corentin
authors.initials: C
authors.lastname: Le Magueresse
authors.forename: Marina
authors.initials: M
authors.lastname: Frank
authors.forename: Klaus
authors.initials: K
authors.lastname: Willecke
authors.forename: Hannah
authors.initials: H
authors.lastname: Monyer
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D001973:Bromodeoxyuridine; D049109:Cell Proliferation; D017630:Connexins / Q000378:metabolism*; D020547:Lateral Ventricles / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D058953:Neural Stem Cells / Q000502:physiology*; D055495:Neurogenesis / Q000502:physiology*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D001973:Bromodeoxyuridine; D017630:Connexins; C499317:Ascl1 protein, mouse; C082920:connexin 45