title: Bio-imaging and cancer targeting with glycoproteins and N-glycans.
abstract: Positron emission tomography (PET) is a non-invasive method that visualizes the locations and levels of radiotracer accumulation with high imaging contrast. A new labeling method using 6π-electrocyclization was developed for PET and fluorescence labeling. The high reactivity of the new method enabled the labeling of peptides and proteins at low concentration within a short period. PET or fluorescent imaging of the labeled glycoproteins and N-glycan glycoclusters successfully visualized their dynamics and bio-distribution depending on the glycan structures. The trafficking of the lymphocytes was also clearly visualized by the non-invasive fluorescence imaging. The present electrocyclization protocol was also applied to the cell-surface engineering by the oligosaccharides; the lymphocytes introduced with N-glycan successfully targeted the tumor tissue implanted in the nude mice.
authors.affiliation: Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka-shi, Osaka 560-0043, Japan. koichi@chem.sci.osaka-u.ac.jp
authors.forename: Koichi
authors.initials: K
authors.lastname: Fukase
authors.forename: Katsunori
authors.initials: K
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D008214:Lymphocytes / Q000166:cytology; D009369:Neoplasms / Q000175:diagnosis*; D061848:Optical Imaging / Q000379:methods*; D011134:Polysaccharides / Q000032:analysis*; D049268:Positron-Emission Tomography / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D006023:Glycoproteins; D011134:Polysaccharides