title: Purification and biochemical characterization of a novel alkaline (phospho)lipase from a newly isolated Fusarium solani strain.
abstract: An extracellular lipase from Fusarium solani strain (F. solani lipase (FSL)) was purified to homogeneity by ammonium sulphate precipitation, gel filtration and anion exchange chromatography. The purified enzyme has a molecular mass of 30 kDa as estimated by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The 12 NH(2)-terminal amino acid residues showed a high degree of homology with a putative lipase from the fungus Necteria heamatoccocae. It is a serine enzyme, like all known lipases from different origins. Interestingly, FSL has not only lipase activity but also a high phospholipase activity which requires the presence of Ca(2+) and bile salts. The specific activities of FSL were about 1,610 and 2,414 U/mg on olive oil emulsion and egg-yolk phosphatidylcholine as substrates, respectively, at pH 8.0 and 37 °C. The (phospho)lipase enzyme was stable in the pH range of 5-10 and at temperatures below 45 °C.
authors.affiliation: Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS route de Soukra, BPW 3038, Sfax, Tunisia.
authors.forename: Raida
authors.initials: R
authors.lastname: Jallouli
authors.forename: Fatma
authors.initials: F
authors.lastname: Khrouf
authors.forename: Ahmed
authors.initials: A
authors.lastname: Fendri
authors.forename: Tahar
authors.initials: T
authors.lastname: Mechichi
authors.forename: Youssef
authors.initials: Y
authors.lastname: Gargouri
authors.forename: Sofiane
authors.initials: S
authors.lastname: Bezzine
mesh_terms: D000595:Amino Acid Sequence; D001647:Bile Acids and Salts / Q000494:pharmacology; D055162:Biocatalysis / Q000187:drug effects; D002118:Calcium / Q000494:pharmacology; D004795:Enzyme Stability; D005670:Fusarium / Q000201:enzymology* / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007783:Lactones / Q000494:pharmacology; D000077403:Orlistat; D010740:Phospholipases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D013696:Temperature; D014197:Trees / Q000382:microbiology; D014934:Wood / Q000382:microbiology
chemical_list: D001647:Bile Acids and Salts; D007783:Lactones; D000077403:Orlistat; D010740:Phospholipases; D002118:Calcium