title: A mouse mammary epithelial cell line permissive for highly efficient human adenovirus growth.
abstract: Although a few immunocompetent animal models to study the immune response against human adenoviruses (HAdV) are available, such as Syrian hamsters and cotton rats, HAdV replication is several logs lower compared to human control cells. We have identified a non-transformed mouse epithelial cell line (NMuMG) where HAdV-2 gene expression and progeny formation was as efficient as in the highly permissive human A549 cells. HAdV from species, D and E (HAdV-37 and HAdV-4, respectively) also caused a rapid cytopathic effect in NMuMG cells, while HAdV from species A, B1, B2 and F (HAdV-12, HAdV-3, HAdV-11 and HAdV-41, respectively) failed to do so. NMuMG cells might therefore be useful in virotherapy research and the analysis of antiviral defense mechanisms and the determination of toxicity, biodistribution and specific antitumour activity of oncolytic HAdV vectors.
authors.affiliation: Department of Medical Biochemistry and Microbiology, Uppsala University, Sweden.
authors.forename: Chengjun
authors.initials: C
authors.lastname: Wu
authors.forename: Daniel
authors.initials: D
authors.lastname: Öberg
authors.forename: Asif
authors.initials: A
authors.lastname: Rashid
authors.forename: Rajesh
authors.initials: R
authors.lastname: Gupta
authors.forename: Marco
authors.initials: M
authors.lastname: Mignardi
authors.forename: Staffan
authors.initials: S
authors.lastname: Johansson
authors.forename: Göran
authors.initials: G
authors.lastname: Akusjärvi
authors.forename: Catharina
authors.initials: C
authors.lastname: Svensson
mesh_terms: D000260:Adenoviruses, Human / Q000145:classification / Q000472:pathogenicity / Q000502:physiology*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D004847:Epithelial Cells / Q000821:virology*; D005260:Female; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D008321:Mammary Glands, Animal / Q000166:cytology*; D051379:Mice; D014779:Virus Replication