title: Analysis of microRNA and gene expression profiling in triazole fungicide-treated HepG2 cell line.
abstract: MicroRNA (miRNA) plays an important role in various diseases and in cellular and molecular responses to toxicants. In the present study, we investigated differential expression of miRNAs in response to three triazole fungicides (myclobutanil, propiconazole, and triadimefon). The human hepatoma cell line (HepG2) was treated with the above triazoles for 3 h or 48 h. miRNA-based microarray experiments were carried out using the Agilent human miRNA v13 array. At early exposure (3h), six miRNAs were differentially expressed and at late exposure (48 h), three miRNAs were significantly expressed. Overall, this study provides an array of potential biomarkers for the above triazole fungicides. Furthermore, these miRNAs induced by triazoles could be the foundation for the development of a miRNA-based toxic biomarker library that can predict environmental toxicity.
authors.affiliation: Department of Biochemistry, Hanyang University, Sangnok-gu, Ansan, Gyeonggi-do, Republic of Korea.
authors.forename: Yu Ri
authors.initials: YR
authors.lastname: An
authors.forename: Seung Jun
authors.initials: SJ
authors.lastname: Kim
authors.forename: Moon-Ju
authors.initials: MJ
authors.lastname: Oh
authors.forename: Hyun-Mi
authors.initials: HM
authors.lastname: Kim
authors.forename: Il-Seob
authors.initials: IS
authors.lastname: Shim
authors.forename: Pil-Je
authors.initials: PJ
authors.lastname: Kim
authors.forename: Kyunghee
authors.initials: K
authors.lastname: Choi
authors.forename: Seung Yong
authors.initials: SY
authors.lastname: Hwang
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D005659:Fungicides, Industrial / Q000633:toxicity*; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000187:drug effects*; D056945:Hep G2 Cells; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009570:Nitriles / Q000008:administration & dosage / Q000633:toxicity; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D013997:Time Factors; D014230:Triazoles / Q000008:administration & dosage / Q000633:toxicity
chemical_list: D015415:Biomarkers; D005659:Fungicides, Industrial; D035683:MicroRNAs; D009570:Nitriles; D014230:Triazoles; C045950:propiconazole; C032910:triadimefon; C446685:systhane