title: [Establishment of a high throughput screening model of cannabinoid receptor 2 agonist].
abstract: OBJECTIVE
  To establish a drug screening model of CB(2) agonist in vitro based on signal regulation pathway.
  
  
  METHODS
  Plasmid pIRES(2)-EGFP-CB(2), pGL(4), 29[luc2P/CRE/Hygro] and PRL-TK were co-transfected into CHO cells in 96 wells plate, to screen agonists of CB(2) receptor by detecting the expressing levels of dual luciferase activity. The concentration and acting time of the agonist were optimized and the stability of the model were investigated.
  
  
  RESULTS
  The largest relative induction activity was obtained after 8h drug administration. Establishment of a high throughput screening model for CB(2) receptor agonist. The Z' factor is 0.75 demonstrating its perfect stability.
  
  
  CONCLUSIONS
  Successfully establish a drug screening model of CB(2) agonist, which provided a basis for searching valid material from traditional Chinese medicine.
authors.affiliation: Department of Pharmacology, Chongqing Medical University, Chongqing, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhi
authors.initials: Z
authors.lastname: Dong
authors.forename: Xiao-qiu
authors.initials: XQ
authors.lastname: Xiao
authors.forename: Hong
authors.initials: H
authors.lastname: Tang
authors.forename: Le-le
authors.initials: LL
authors.lastname: Wang
authors.forename: Yu-jie
authors.initials: YJ
authors.lastname: Cheng
mesh_terms: D000818:Animals; D016466:CHO Cells; D063386:Cannabinoid Receptor Agonists / Q000494:pharmacology*; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D057166:High-Throughput Screening Assays; D043885:Receptor, Cannabinoid, CB2 / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results
chemical_list: D063386:Cannabinoid Receptor Agonists; D043885:Receptor, Cannabinoid, CB2; D011993:Recombinant Fusion Proteins