title: Anti-inflammatory activity of a honey flavonoid extract on lipopolysaccharide-activated N13 microglial cells.
abstract: Neuroinflammation is an important contributor to pathogenesis of age-related neurodegenerative disorders such as Alzheimer's or Parkinson's disease. Accumulating evidence indicates that inhibition of microglia-mediated neuroinflammation may become a reliable protective strategy for neurodegenerative processes. Flavonoids, widely distributed in the vegetable kingdom and in foods such as honey, have been suggested as novel therapeutic agents for the reduction of the deleterious effects of neuroinflammation. The present study investigated the potential protective effect of a honey flavonoid extract (HFE) on the production of pro-inflammatory mediators by lipopolysaccharide-stimulated N13 microglia. The results show that HFE significantly inhibited the release of pro-inflammatory cytokines such as TNF-α and IL-1β. The expressions of iNOS and the production of reactive oxygen intermediates (ROS) were also significantly inhibited. Accordingly, the present study demonstrates that HFE is a potent inhibitor of microglial activation and thus a potential preventive-therapeutic agent for neurodegenerative diseases involving neuroinflammation.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Sevilla, Sevilla, Spain.
authors.forename: Manila
authors.initials: M
authors.lastname: Candiracci
authors.forename: Elena
authors.initials: E
authors.lastname: Piatti
authors.forename: María
authors.initials: M
authors.lastname: Dominguez-Barragán
authors.forename: Daniel
authors.initials: D
authors.lastname: García-Antrás
authors.forename: Bruno
authors.initials: B
authors.lastname: Morgado
authors.forename: Diego
authors.initials: D
authors.lastname: Ruano
authors.forename: Juan F
authors.initials: JF
authors.lastname: Gutiérrez
authors.forename: Juan
authors.initials: J
authors.lastname: Parrado
authors.forename: Angélica
authors.initials: A
authors.lastname: Castaño
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D016207:Cytokines / Q000096:biosynthesis / Q000378:metabolism; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D006722:Honey / Q000032:analysis*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D005419:Flavonoids; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species