title: MicroRNA-657 promotes tumorigenesis in hepatocellular carcinoma by targeting transducin-like enhancer protein 1 through nuclear factor kappa B pathways.
abstract: Growing evidence indicates that deregulation of microRNAs (miRNAs) contributes to tumorigenesis. Dysregulation of miR-657 has been observed in several types of cancers, but its biological function is still largely unknown. Our results showed that miR-657 expression can be induced by hepatitis viral proteins and is significantly increased in hepatocellular carcinoma (HCC) tissues. Moreover, introduction of miR-657 dramatically increases proliferation and colony formation of HCC cells in vitro and induces tumor development in immunodeficient mice. Further studies showed that miR-657 directly targets the transducin-like enhancer protein 1 (TLE1) 3' untranslated region (UTR) and activates nuclear factor kappa B (NF-κB) pathways that contribute to hepatocarcinogenesis.
  
  
  CONCLUSIONS
  This study identified a mechanism whereby miRNA-657 contributed to HCC through novel cancer pathways and provides new insights into the potential molecular mechanisms of hepatic carcinogenesis.
authors.affiliation: Department of Molecular Pharmacology, Beckman Research Institute, Duarte, CA, USA.
authors.forename: Lisheng
authors.initials: L
authors.lastname: Zhang
authors.forename: Lixin
authors.initials: L
authors.lastname: Yang
authors.forename: Xiyong
authors.initials: X
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Lufen
authors.initials: L
authors.lastname: Chang
authors.forename: Linling
authors.initials: L
authors.lastname: Chen
authors.forename: Sofia
authors.initials: S
authors.lastname: Loera
authors.forename: Peiguo
authors.initials: P
authors.lastname: Chu
authors.forename: Wei-Chien
authors.initials: WC
authors.lastname: Huang
authors.forename: Yun-Ru
authors.initials: YR
authors.lastname: Liu
authors.forename: Yun
authors.initials: Y
authors.lastname: Yen
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D002478:Cells, Cultured; D056970:Co-Repressor Proteins; D004195:Disease Models, Animal; D018450:Disease Progression; D006801:Humans; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D014162:Transfection; D014183:Transplantation, Heterologous
chemical_list: D056970:Co-Repressor Proteins; C582070:MIRN657 microRNA, human; D035683:MicroRNAs; D016328:NF-kappa B; D012097:Repressor Proteins; C499043:TLE1 protein, human