title: In vitro propagation of Hydrangea spp.
abstract: Hydrangea (Hortensia) is a highly popular ornamental plant for garden decoration, and now it is commercially produced for cut flower branches. For in vitro culture, Murashige and Skoog medium supplemented with BA (0.25 mg/L) and sucrose (30 g/L) was used. Culture conditions were 23 ± 1°C of temperature, light intensity of 35 μmol/m(2)/s P.P.F.D., and 16/8 h day/night photoperiod. Following shoot proliferation, the in vitro rooting frequency was 100% on a medium containing NAA 0.5 mg/L. However, 95% direct in vivo rooting was achieved by dipping microcuttings in a 5,000 ppm K-IBA solution which were transferred afterward to a glasshouse for acclimatization. After 21 days, fully acclimatized and well-established plants were obtained, suitable for commercialization. Furthermore, leaf fragments derived from in vitro plantlets were cultured for callus induction and adventitious shoot regeneration.
authors.affiliation: Ornamental Plants Research Unit, CRA-FSO, Sanremo, Italy. barbara.ruffoni@entecra.it
authors.forename: Barbara
authors.initials: B
authors.lastname: Ruffoni
authors.forename: Ermanno
authors.initials: E
authors.lastname: Sacco
authors.forename: Marco
authors.initials: M
authors.lastname: Savona
mesh_terms: D000064:Acclimatization; D003470:Culture Media / Q000737:chemistry; D046508:Culture Techniques / Q000379:methods*; D031966:Hydrangea / Q000254:growth & development* / Q000502:physiology; D007211:Indoles / Q000737:chemistry; D018515:Plant Leaves / Q000254:growth & development / Q000502:physiology; D018517:Plant Roots / Q000254:growth & development / Q000502:physiology; D012038:Regeneration; D013242:Sterilization; D014867:Water / Q000737:chemistry
chemical_list: D003470:Culture Media; D007211:Indoles; D014867:Water; C014612:indolebutyric acid