title: A comparison of the accuracy of Illumina HumanHT-12 v3 Expression BeadChip and TaqMan qRT-PCR gene expression results in patient samples from the Tampere Vascular Study.
abstract: OBJECTIVE
  This study was set up to compare the accuracy, sensitivity and specificity of gene expression results between the Illumina HumanHT-12 v3 Expression BeadChip (GWE) and the TaqMan qRT-PCR low-density array (LDA) in atherosclerotic plaques.
  
  
  METHODS
  Gene expression levels of 196 genes were determined from 22 atherosclerotic samples and 6 controls with both the GWE and the LDA.
  
  
  RESULTS
  The accuracies of GWE in comparison to that of qRT-PCR for absolute fold changes (FC) 1.2, 1.6, 2.0 and 3.0 between cases and controls were 73.5%, 79.1%, 72.4% and 60.7%, respectively. The correlation of expression measurements between these methods was good (r = 0.87, y = 0.151 + 0.586x), and the Bland-Altman plot showed that for highly up- or down-regulated transcripts, GWE yields lower absolute FC values than LDA.
  
  
  CONCLUSIONS
  Gene expression results obtained with the GWE are replicated with high accuracy in LDA, even for technically demanding atherosclerotic samples.
authors.affiliation: Department of Clinical Chemistry, Tampere University Hospital, Fimlab Laboratories and University of Tampere, School of Medicine, Finland. emma.raitoharju@uta.fi
authors.forename: Emma
authors.initials: E
authors.lastname: Raitoharju
authors.forename: Ilkka
authors.initials: I
authors.lastname: Seppälä
authors.forename: Leo-Pekka
authors.initials: LP
authors.lastname: Lyytikäinen
authors.forename: Mari
authors.initials: M
authors.lastname: Levula
authors.forename: Niku
authors.initials: N
authors.lastname: Oksala
authors.forename: Norman
authors.initials: N
authors.lastname: Klopp
authors.forename: Thomas
authors.initials: T
authors.lastname: Illig
authors.forename: Reijo
authors.initials: R
authors.lastname: Laaksonen
authors.forename: Mika
authors.initials: M
authors.lastname: Kähönen
authors.forename: Terho
authors.initials: T
authors.lastname: Lehtimäki
mesh_terms: D015870:Gene Expression; D055106:Genome-Wide Association Study / Q000379:methods*; D006801:Humans; D058226:Plaque, Atherosclerotic / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity