title: Live birth after SrCl(2) oocyte activation in previous repeated failed or low fertilization rates after ICSI of frozen-thawed testicular spermatozoa: case report.
abstract: OBJECTIVE
  To report a live birth resulting after strontium chloride (SrCl(2)) oocyte activation in a couple with complete fertilization failure or low fertilization rates following intracytoplasmic sperm injection (ICSI) of frozen-thawed testicular spermatozoa.
  
  
  METHODS
  The couple underwent ICSI of frozen-thawed testicular spermatozoa. After ICSI, the oocytes were artificially activated by SrCl(2) because the results of fertilization were not satisfactory in the previous cycles. The main outcome measures were fertilization, pregnancy, and birth.
  
  
  RESULTS
  In the first and second cycles performed previously at another clinic, fertilization rates were 9.1 % and 0.0 %, respectively. In the third cycle, 31 metaphase II oocytes were retrieved. After sperm injection, all of the oocytes were stimulated using SrCl(2) for activation. Sixteen oocytes were fertilized (51.6 %), and a single embryo was transferred into the uterus on Day 3. A healthy girl weighing 2750 g was born at 40 weeks of gestation by caesarean section.
  
  
  CONCLUSIONS
  This result suggests that SrCl(2) could be useful for oocyte fertilization in case of repeated complete fertilization failure or low fertilization rates following ICSI of frozen-thawed testicular spermatozoa.
authors.affiliation: In Vitro Fertilization Center, Maria Fertility Hospital, 121-1 Garak-dong, Songpa-gu, Seoul, 138-160, Korea. daniellove@mariababy.com
authors.forename: Jun-Woo
authors.initials: JW
authors.lastname: Kim
authors.forename: Jung-Lim
authors.initials: JL
authors.lastname: Choi
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Yang
authors.forename: San-Hyun
authors.initials: SH
authors.lastname: Yoon
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Jung
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Lim
mesh_terms: D000328:Adult; D015925:Cryopreservation; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007246:Infertility / Q000188:drug therapy; D050498:Live Birth; D008297:Male; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000166:cytology; D013324:Strontium / Q000008:administration & dosage*; D013737:Testis / Q000166:cytology
chemical_list: C025700:strontium chloride; D013324:Strontium