title: ChIP for identification of p53 responsive DNA promoters.
abstract: Chromatin immunoprecipitation assay (ChIP) has been frequently used to determine whether a transcriptional regulator can bind to a specific DNA element in the chromatin content of cells. Here, we describe a detailed protocol for this assay with hands-on tips based on our own experience in working on the transcriptional regulator and tumor suppressor p53.
authors.affiliation: Department of Biochemistry & Molecular Biology and Simon Cancer Center, Indiana University School of Medicine, Indianapolis, IN, USA.
authors.forename: Jun-Ming
authors.initials: JM
authors.lastname: Liao
authors.forename: Hua
authors.initials: H
authors.lastname: Lu
mesh_terms: D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D053938:DNA Fragmentation; D005786:Gene Expression Regulation; D006801:Humans; D011401:Promoter Regions, Genetic; D011485:Protein Binding / Q000235:genetics; D014158:Transcription, Genetic; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D016159:Tumor Suppressor Protein p53; D004247:DNA