title: Transient receptor potential N (TRPN1) from Xenopus interacts with the penta-EF-hand protein peflin.
abstract: TRPN1 is a candidate mechanotransduction channel in Drosophila and Caenorhabditis elegans, also present in hair cells of lower vertebrates. At its N-terminal cytoplasmic tail it contains 28 ankyrin repeats. We performed a yeast two-hybrid screen with the N-terminal ankyrin repeats of Xenopus TRPN1 as bait and identified the Penta-EF-hand protein peflin as a putative interaction partner. We confirmed this interaction by GST pulldown assays and by co-localization in an epithelial cell model. Collectively, our study identifies peflin as an interaction partner of TRPN1 in vitro.
authors.affiliation: Department of Physiology, RWTH Aachen University, 52074 Aachen, Germany.
authors.forename: Dominik
authors.initials: D
authors.lastname: Wiemuth
authors.forename: Lena
authors.initials: L
authors.lastname: van de Sandt
authors.forename: Rudolf
authors.initials: R
authors.lastname: Herr
authors.forename: Stefan
authors.initials: S
authors.lastname: Gründer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017089:Ankyrin Repeat; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D020832:EF Hand Motifs; D006198:Hair Cells, Auditory / Q000378:metabolism; D050051:Transient Receptor Potential Channels / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis / Q000378:metabolism*
chemical_list: D002135:Calcium-Binding Proteins; C578336:LOC100492885 protein, Xenopus; D050051:Transient Receptor Potential Channels; D029867:Xenopus Proteins; C578335:peflin protein, Xenopus