title: Data management in thrombin generation.
abstract: To obtain a thrombin generation (TG) curve from the conversion of added fluorogenic substrate, thrombin concentrations are to be derived from the observed velocity of increase of fluorescence (dF/dt). The relation between velocity and thrombin concentration varies during the experiment because substrate is consumed and because fluorescence is not linear with the concentration of product. Here we review the techniques that we developed to:
authors.affiliation: Cardiovascular Research Institute Maastricht (CARIM) and Synapse BV., Maastricht, The Netherlands. HC.Hemker@Thrombin.Com
authors.forename: H Coenraad
authors.initials: HC
authors.lastname: Hemker
authors.forename: R
authors.initials: R
authors.lastname: Kremers
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D001777:Blood Coagulation; D001780:Blood Coagulation Tests / Q000592:standards; D002138:Calibration; D006801:Humans; D007700:Kinetics; D017711:Nonlinear Dynamics; D011237:Predictive Value of Tests; D011516:Prothrombin / Q000378:metabolism; D012015:Reference Standards; D013050:Spectrometry, Fluorescence; D013917:Thrombin / Q000378:metabolism*; D000511:alpha-Macroglobulins / Q000378:metabolism
chemical_list: D015415:Biomarkers; D000511:alpha-Macroglobulins; D011516:Prothrombin; D013917:Thrombin