title: Adhesion and invasion to duck embryo fibroblast cells by Riemerella anatipestifer.
abstract: Here, we investigated adhesion and invasion of Riemerella anatipestifer (RA) to primary duck embryo ﬁbroblast (DEF) cells. The ability of RA to adhere to, and more importantly, to invade DEF cells was demonstrated by using a gentamicin invasion assay and was confirmed by transmission electron microscopy (TEM). Adhesion of RA could be found by TEM after 1 h of inoculation. Both apoptosis and necrocytosis of DEF were indicated by TEM after 10 h of incubation, which suggested a complex mechanism of DEF cell death induced by RA. Our results showed that internalized RA had the ability to leave the DEF cells. Inhibition studies indicated that RA proteins play a role in adhesion. Moreover, invasion of RA to DEF cells was shown to require rearrangement of actin microﬁlaments and microtubular cytoskeletal elements. Because the adhesion and invasion ability of RA to DEF cells could be demonstrated in vitro, similar processes might occur in vivo, where DEF cells play a crucial role in the diffusion of RA in ducks.
authors.affiliation: Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan 611130, P.R. China.
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: D K
authors.initials: DK
authors.lastname: Zhu
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.forename: M S
authors.initials: MS
authors.lastname: Wang
authors.forename: A C
authors.initials: AC
authors.lastname: Cheng
authors.forename: R Y
authors.initials: RY
authors.lastname: Jia
authors.forename: S
authors.initials: S
authors.lastname: Chen
authors.forename: F
authors.initials: F
authors.lastname: Liu
authors.forename: Q M
authors.initials: QM
authors.lastname: Yang
authors.forename: X Y
authors.initials: XY
authors.lastname: Chen
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004372:Ducks / Q000196:embryology*; D005347:Fibroblasts / Q000382:microbiology*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D060494:Riemerella / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins