title: [Single nucleotide polymorphism in flanking region of miR-30c influences the maturing process of miR-30c in lung carcinoma].
abstract: OBJECTIVE
  To investigate the effect of a common polymorphism rs928508(A/G) in flanking region of miR-30c on the expression of pri, pre and mature miR-30c, and discuss the effect of this polymorphism on the maturing process of miR-30c in lung carcinoma.
  
  
  METHODS
  The pGL3-promoter-miR-30c-A and pGL3-promoter-miR-30c-G luciferase plasmids were created containing A or G allele of miR-30c flanking region. Taqman assay was used to genotype rs928508 polymorphism in 50 lung cancer tissues. RT-PCR was performed to determine the expression of pri-miR-30c, pre-miR-30c, mature miR-30c and miR-30c host gene NFYC in the 50 lung cancer tissues.
  
  
  RESULTS
  The luciferase expression level of the pGL3-promoter-miR-30c-A construct group was not significantly different compared with that in the the pGL3-promoter-miR-30c-G construct group (A549 cells, P = 0.758; 293A cells, P = 0.554; CHO cells, P = 0.175). The results demonstrated that rs928508(A/G) variant had no effect on the transcriptional regulation of pri-miR-30c. In the genotype-phenotype collection analysis of the 50 lung cancer tissues, the expression of pre-miR-30c and mature miR-30c for rs928508 AG/GG genotypes showed significantly lower levels compared with those in the AA genotype (P = 0.009, P = 0.011). However, the expression of pri-miR-30c showed no significant difference between AG/GG genotypes and AA genotype. Similarly, the expression of host NFYC gene was correlated with pri-miR-30c, showed no significant difference between AG/GG genotypes and AA genotype.
  
  
  CONCLUSIONS
  The rs928508(A/G) polymorphism in flanking region of miR-30c could influence the processing from pri-miR-30c to mature miR-30c, but does not influence the transcription of pri-miR-30c.
authors.affiliation: Department of Epidemiology and Biostatistics, Nanjing Medical University, Nanjing, China.
authors.forename: Jia-ping
authors.initials: JP
authors.lastname: Chen
authors.forename: Yao
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhi-bin
authors.initials: ZB
authors.lastname: Hu
authors.forename: Hong-bing
authors.initials: HB
authors.lastname: Shen
mesh_terms: D000818:Animals; D023081:CCAAT-Binding Factor / Q000235:genetics* / Q000378:metabolism; D016466:CHO Cells; D045744:Cell Line, Tumor; D006224:Cricetinae; D005838:Genotype; D057809:HEK293 Cells; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic
chemical_list: D023081:CCAAT-Binding Factor; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; C469358:NFYC protein, human