title: Antigen 85A and mycobacterial DNA-binding protein 1 are targets of immunoglobulin G in individuals with past tuberculosis.
abstract: Development of accurate methods for predicting progression of tuberculosis (TB) from the latent state is recognized as vitally important in controlling TB, because a majority of cases develop from latent infections. Past TB that has never been treated has a higher risk of progressing than does latent Mycobacterium tuberculosis infection in patients who have previously received treatment. Antibody responses against 23 kinds of M. tuberculosis proteins in individuals with past TB who had not been medicated were evaluated. These individuals had significantly higher concentrations of antibodies against Antigen 85A and mycobacterial DNA-binding protein 1 (MDP1) than did those with active TB and uninfected controls. In addition, immunohistochemistry revealed colocalization of tubercle bacilli, antigen 85 and MDP1 inside tuberculous granuloma lesions in an asymptomatic subject, showing that M. tuberculosis in lesions expresses both antigen 85 and MDP1. Our study suggests the potential usefulness of measuring antibody responses to antigen 85A and MDP1 for assessing the risk of TB progression.
authors.affiliation: Department of Bacteriology, Osaka City University Graduate School of Medicine, 1-4-3 Abeno-ku, Osaka 545-8585, Japan.
authors.forename: Mayuko
authors.initials: M
authors.lastname: Osada-Oka
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Tateishi
authors.forename: Yukio
authors.initials: Y
authors.lastname: Hirayama
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Ozeki
authors.forename: Mamiko
authors.initials: M
authors.lastname: Niki
authors.forename: Seigo
authors.initials: S
authors.lastname: Kitada
authors.forename: Ryoji
authors.initials: R
authors.lastname: Maekura
authors.forename: Kunio
authors.initials: K
authors.lastname: Tsujimura
authors.forename: Yukio
authors.initials: Y
authors.lastname: Koide
authors.forename: Naoya
authors.initials: N
authors.lastname: Ohara
authors.forename: Taro
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Sohkichi
authors.initials: S
authors.lastname: Matsumoto
mesh_terms: D000217:Acyltransferases / Q000276:immunology*; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology*; D058070:Asymptomatic Diseases; D001426:Bacterial Proteins / Q000276:immunology*; D004268:DNA-Binding Proteins / Q000276:immunology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007150:Immunohistochemistry; D055985:Latent Tuberculosis / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011379:Prognosis; D014376:Tuberculosis / Q000175:diagnosis / Q000276:immunology* / Q000473:pathology; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D007074:Immunoglobulin G; C403010:MDP1 protein, Mycobacterium; D000217:Acyltransferases; C112580:antigen 85A, Mycobacterium tuberculosis