title: IL-4 and IFN-γ induced by human immunodeficiency virus vaccine in a schistosome infection model.
abstract: The co-infection of HIV and helminth parasites, such as Schistosoma spp, has increased in sub-Saharan Africa. Many HIV vaccine candidate studies have been completed or are in ongoing clinical trials, but it is not clear how HIV vaccines might affect the course of schistosome infections. In this study, we immunized S. mansoni-infected mice with an efficient DNA vaccine that included HIV gag. Using this model, we found that Th2 cytokines, such as IL-4 and IL-13, were highly induced after schistosome infection. Treatment of infected mice with the HIV DNA vaccine resulted in a significant attenuation of this rise in IL-13 expression and an increase in expression of the Th1 cytokine, TNF-α. However, vaccine administration did not significantly influence the expression of IL-4, or IFN-γ, and did not affect T cell proliferative capacity. Interestingly, the IL-4 (+) IFN-γ (+) phenotype appears in schistosome-infected mice that received HIV vaccination, and is associated with the expression of transcription factors GATA3 (+) T-bet (+) in these mice. These studies indicate that DNA vaccination can have an impact on ongoing chronic infection.
authors.affiliation: Department of Pathology and Laboratory Medicine; University of Pennsylvania School of Medicine; Philadelphia, PA USA.
authors.forename: Jiangmei
authors.initials: J
authors.lastname: Yin
authors.forename: Anlan
authors.initials: A
authors.lastname: Dai
authors.forename: Tatiana
authors.initials: T
authors.lastname: Arango
authors.forename: Ravi S
authors.initials: RS
authors.lastname: Kasinathan
authors.forename: Robert M
authors.initials: RM
authors.lastname: Greenberg
authors.forename: Jean D
authors.initials: JD
authors.lastname: Boyer
mesh_terms: D016915:AIDS Vaccines / Q000627:therapeutic use*; D000818:Animals; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D015847:Interleukin-4 / Q000378:metabolism*; D051379:Mice; D012555:Schistosomiasis mansoni / Q000276:immunology* / Q000628:therapy*; D019444:Vaccines, DNA / Q000627:therapeutic use
chemical_list: D016915:AIDS Vaccines; D019444:Vaccines, DNA; D015847:Interleukin-4; D007371:Interferon-gamma