title: Chromosomal Aberrations in ETV6/RUNX1-positive Childhood Acute Lymphoblastic Leukemia using 244K Oligonucleotide Array Comparative Genomic Hybridization.
abstract: BACKGROUND
  Acute lymphoblastic leukemia (ALL) is a heterogeneous form of hematological cancer consisting of various subtypes. We are interested to study the genetic aberration in precursor B-cell ALL with specific t(12;21) translocation in childhood ALL patients. A high resolution 244K array-based Comparative Genomic Hybridization (array-CGH) was used to study eleven ETV6/RUNX1-positive childhood acute lymphoblastic leukemia (ALL) patients.
  
  
  RESULTS
  155 chromosomal aberrations (119 losses, 36 gains) were reported in the array findings, corresponding to 76.8% deletions and 23.2% amplifications. The ETV6 gene deletion occurred in 4 of the patients, corresponding to 45% of the sample. The most common alterations above 1 Mb were deletion 6q (13%), 12p (12%) and 9p (8%), and duplication 4q (6%) and Xq (4%). Other genes important in ALL were also identified in this study including RUNX1, CDKN2A, FHIT, and PAX5. The array-CGH technique was able to detect microdeletion as small as 400 bp.
  
  
  CONCLUSIONS
  The results demonstrate the usefulness of high resolution array-CGH as a complementary tool in the investigation of ALL.
authors.affiliation: Hematology Unit, Cancer Research Center, Institute for Medical Research, Kuala Lumpur, 50588, Malaysia. zubaidah@imr.gov.my.
authors.forename: Zubaidah
authors.initials: Z
authors.lastname: Zakaria
authors.forename: Mohd Fadly Md
authors.initials: MF
authors.lastname: Ahid
authors.forename: Azli
authors.initials: A
authors.lastname: Ismail
authors.forename: Ten Sew
authors.initials: TS
authors.lastname: Keoh
authors.forename: Nooraisyah Mohamad
authors.initials: NM
authors.lastname: Nor
authors.forename: Nor Rizan
authors.initials: NR
authors.lastname: Kamaluddin
authors.forename: Ezalia
authors.initials: E
authors.lastname: Esa
authors.forename: Lam Kah
authors.initials: LK
authors.lastname: Yuen
authors.forename: Eni Juraida Abdul
authors.initials: EJ
authors.lastname: Rahman
authors.forename: Raudhawati
authors.initials: R
authors.lastname: Osman