title: No evidence of the genotoxic potential of gold, silver, zinc oxide and titanium dioxide nanoparticles in the SOS chromotest.
abstract: Gold nanoparticles (Au NPs), silver nanoparticles (Ag NPs), zinc oxide nanoparticles (ZnO NPs) and titanium dioxide nanoparticles (TiO2 NPs) are widely used in cosmetic products such as preservatives, colorants and sunscreens. This study investigated the genotoxicity of Au NPs, Ag NPs, ZnO NPs and TiO2 NPs using the SOS chromotest with Escherichia coli PQ37. The maximum exposure concentrations for each nanoparticle were 3.23 mg l(-1) for Au NPs, 32.3 mg l(-1) for Ag NPs and 100 mg l(-1) for ZnO NPs and TiO2 NPs. Additionally, in order to compare the genotoxicity of nanoparticles and corresponding dissolved ions, the ions were assessed in the same way as nanoparticles. The genotoxicity of the titanium ion was not assessed because of the extremely low solubility of TiO2 NPs. Au NPs, Ag NPs, ZnO NPs, TiO2 NPs and ions of Au, Ag and Zn, in a range of tested concentrations, exerted no effects in the SOS chromotest, evidenced by maximum IF (IFmax) values of below 1.5 for all chemicals. Owing to the results, nanosized Au NPs, Ag NPs, ZnO NPs, TiO2 NPs and ions of Au, Ag and Zn are classified as non-genotoxic on the basis of the SOS chromotest used in this study. To the best of our knowledge, this is the first study to evaluate the genotoxicity of Au NPs, Ag NPs, ZnO NPs and TiO2 NPs using the SOS chromotest.
authors.affiliation: Department of Environmental Science, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Korea.
authors.forename: Sun-Hwa
authors.initials: SH
authors.lastname: Nam
authors.forename: Shin Woong
authors.initials: SW
authors.lastname: Kim
authors.forename: Youn-Joo
authors.initials: YJ
authors.lastname: An
mesh_terms: D004249:DNA Damage / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D006046:Gold / Q000737:chemistry / Q000633:toxicity; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D046529:Microscopy, Electron, Transmission; D009152:Mutagenicity Tests; D012834:Silver / Q000737:chemistry / Q000633:toxicity; D014025:Titanium / Q000737:chemistry / Q000633:toxicity; D015034:Zinc Oxide / Q000737:chemistry / Q000633:toxicity
chemical_list: C009495:titanium dioxide; D012834:Silver; D006046:Gold; D014025:Titanium; D015034:Zinc Oxide