title: High-speed fluorescence imaging and intensity profiling of femtosecond-induced calcium transients.
abstract: We have demonstrated a combined imaging system, where the physiology of biological specimens can be imaged and profiled at 10-20 frames per second whilst undergoing femtosecond laser irradiation. Individual GH3 cells labeled with the calcium fluorophore Fluo-3 were stimulated using a counter-propagating focused femtosecond beam with respect to the imaging system. As a result of the stimulation, calcium waves can be generated in COS cells, and laser-induced calcium oscillations are initiated in the GH3 cells. Single-photon fluorescence images and intensity profiles of the targeted specimens are sampled in real-time using a modified PerkinElmer UltraView LCI microscope.
authors.affiliation: Centre for Micro-Photonics, Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, P.O. Box 218, Hawthorn VIC 3122, Australia.
authors.forename: Daniel
authors.initials: D
authors.lastname: Day
authors.forename: Charles G
authors.initials: CG
authors.lastname: Cranfield
authors.forename: Min
authors.initials: M
authors.lastname: Gu