title: [Analysis of primary metabolites of ranolazine in dog urine by LC-MS(n)].
abstract: Ranolazine and metabolites in dog urine were identified by LC-MS(n). Dog urine samples were collected after ig 30 mg x kg(-1) ranolazine, then the samples were enriched and purified through solid-phase extraction cartridge. The purified samples were analyzed by LC-MS(n). The possible metabolites were discovered by comparing the full scan and SIM chromatograms of the test samples with the corresponding blanks. Seventeen phase I metabolites and fourteen phase II metabolites were identified in dog urine. Three metabolites were identified by comparing with the control article. The metabolites were formed via the following metabolic pathways: O-demethylation, O-dearylation, hydroxylation, N-dealkylation, amide hydrolysis, glucuronidation and sulfation. The LC-MS(n) method is suitable for the rapid identification of drug and its metabolites in biologic samples.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Jing-ya
authors.initials: JY
authors.lastname: Tang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Chun-gang
authors.initials: CG
authors.lastname: Zhang
authors.forename: Di-min
authors.initials: DM
authors.lastname: Wang
authors.forename: Lu
authors.initials: L
authors.lastname: Sun
mesh_terms: D000083:Acetanilides / Q000008:administration & dosage / Q000378:metabolism* / Q000652:urine*; D000284:Administration, Oral; D000818:Animals; D002853:Chromatography, Liquid; D004285:Dogs; D005260:Female; D008297:Male; D010879:Piperazines / Q000008:administration & dosage / Q000378:metabolism* / Q000652:urine*; D000069458:Ranolazine; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D000083:Acetanilides; D010879:Piperazines; D000069458:Ranolazine