title: Spatiotemporal visualization of subcellular dynamics of carbon nanotubes.
abstract: To date, there is no consensus on the relationship between the physicochemical characteristics of carbon nanotubes (CNTs) and their biological behavior; however, there is growing evidence that the versatile characteristics make their biological fate largely unpredictable and remain an issue of limited knowledge. Here we introduce an experimental methodology for tracking and visualization of postuptake behavior and the intracellular fate of CNTs based on the spatial distribution of diffusion values throughout the plant cell. By using raster scan image correlation spectroscopy (RICS), we were able to generate highly quantitative spatial maps of CNTs diffusion in different cell compartments. The spatial map of diffusion values revealed that the uptake of CNTs is associated with important subcellular events such as carrier-mediated vacuolar transport and autophagy. These results show that RICS is a useful methodology to elucidate the intracellular behavior mechanisms of carbon nanotubes and potentially other fluorescently labeled nanoparticles, which is of relevance for the important issues related to the environmental impact and health hazards.
authors.affiliation: Chemical and Life Sciences and Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia. magedserag@yahoo.com
authors.forename: Maged F
authors.initials: MF
authors.lastname: Serag
authors.forename: Kevin
authors.initials: K
authors.lastname: Braeckmans
authors.forename: Satoshi
authors.initials: S
authors.lastname: Habuchi
authors.forename: Noritada
authors.initials: N
authors.lastname: Kaji
authors.forename: Alberto
authors.initials: A
authors.lastname: Bianco
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
mesh_terms: D001343:Autophagy; D001692:Biological Transport; D030008:Catharanthus / Q000166:cytology* / Q000378:metabolism; D004058:Diffusion; D018613:Microscopy, Confocal; D037742:Nanotubes, Carbon / Q000032:analysis*; D061848:Optical Imaging
chemical_list: D037742:Nanotubes, Carbon