title: Investigation of the neuroprotective action of saffron (Crocus sativus L.) in aluminum-exposed adult mice through behavioral and neurobiochemical assessment.
abstract: In the present study, the possible reversal effects of saffron against established aluminum (Al)-toxicity in adult mice, were investigated. Control, Al-treated (50 mg AlCl(3)/kg/day diluted in the drinking water for 5 weeks) and Al+saffron (Al-treatment as previously plus 60 mg saffron extract/kg/day intraperitoneally for the last 6 days), groups of male Balb-c mice were used. We assessed learning/memory, the activity of acetylcholinesterase [AChE, salt-(SS)/detergent-soluble(DS) isoforms], butyrylcholinesterase (BuChE, SS/DS isoforms), monoamine oxidase (MAO-A, MAO-B), the levels of lipid peroxidation (MDA) and reduced glutathione (GSH), in whole brain and cerebellum. Brain Al was determined by atomic absorption spectrometry, while, for the first time, crocetin, the main active metabolite of saffron, was determined in brain after intraperitoneal saffron administration by HPLC. Al intake caused memory impairment, significant decrease of AChE and BuChE activity, activation of brain MAO isoforms but inhibition of cerebellar MAO-B, significant elevation of brain MDA and significant reduction of GSH content. Although saffron extract co-administration had no effect on cognitive performance of mice, it reversed significantly the Al-induced changes in MAO activity and the levels of MDA and GSH. AChE activity was further significantly decreased in cerebral tissues of Al+saffron group. The biochemical changes support the neuroprotective potential of saffron under toxicity.
authors.affiliation: Laboratory of Human and Animal Physiology, Department of Biology, University of Patras, 26504 Patras, Greece. linardaki@upatras.gr
authors.forename: Zacharoula I
authors.initials: ZI
authors.lastname: Linardaki
authors.forename: Malvina G
authors.initials: MG
authors.lastname: Orkoula
authors.forename: Alexandros G
authors.initials: AG
authors.lastname: Kokkosis
authors.forename: Fotini N
authors.initials: FN
authors.lastname: Lamari
authors.forename: Marigoula
authors.initials: M
authors.lastname: Margarity
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000535:Aluminum / Q000032:analysis / Q000493:pharmacokinetics / Q000633:toxicity*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002338:Carotenoids / Q000032:analysis / Q000378:metabolism; D003071:Cognition / Q000187:drug effects; D027622:Crocus / Q000737:chemistry*; D005978:Glutathione / Q000378:metabolism; D007274:Injections, Intraperitoneal; D007858:Learning / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008315:Malondialdehyde / Q000378:metabolism; D008568:Memory / Q000187:drug effects*; D008569:Memory Disorders / Q000139:chemically induced / Q000188:drug therapy; D051379:Mice; D008807:Mice, Inbred BALB C; D008995:Monoamine Oxidase / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress; D010936:Plant Extracts / Q000494:pharmacology; D014801:Vitamin A / Q000031:analogs & derivatives
chemical_list: D018696:Neuroprotective Agents; D010936:Plant Extracts; C487773:trans-sodium crocetinate; D014801:Vitamin A; D002338:Carotenoids; D008315:Malondialdehyde; D000535:Aluminum; D008995:Monoamine Oxidase; D000110:Acetylcholinesterase; D005978:Glutathione