title: [Macrophage apoptosis as a mechanism of pathogenic effect of diphtheria infectious agent].
abstract: OBJECTIVE
  Study of the apoptogenic effect of Corynebacterium diphtheriae toxigenic strains on mice peritoneal macrophages in vitro.
  
  
  METHODS
  Evaluation ofapoptosis induced by Corynebacterium diphtheriae, Corynebacterium pseudodiphtheriticum, Staphylococcus aureus, Streptococcus pyogenes strains was performed by characteristic morphological changes in macrophages in smears stained by azure eosin by Romanovsky-Giemsa.
  
  
  RESULTS
  Apoptogenic activity of diphtheria infectious agent was established to be determined by diphtheria exotoxin at early (after 1 hour) and surface structures and pathogenicity enzymes at later (3 hours) stages of effect.
  
  
  CONCLUSIONS
  The ability of diphtheria infectious agent to cause macrophage apoptosis is one of the mechanisms of realization of its pathogenic properties determined by the effect of diphtheria exotoxin, as well as its surface structures and pathogenicity enzymes. The increase of apoptogenic activity of toxigenic strains of C. diphtheriae in association with S. pyogenes may be a pathogenetic base of formation of prolonged forms of bacteria carriage against the background of chronic ENT pathology.
authors.forename: G G
authors.initials: GG
authors.lastname: Kharseeva
authors.forename: É L
authors.initials: ÉL
authors.lastname: Alutina
authors.forename: G I
authors.initials: GI
authors.lastname: Vasil'eva
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001426:Bacterial Proteins / Q000494:pharmacology; D002478:Cells, Cultured; D003352:Corynebacterium / Q000737:chemistry* / Q000472:pathogenicity; D003353:Corynebacterium diphtheriae / Q000737:chemistry* / Q000472:pathogenicity; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D004167:Diphtheria Toxin / Q000494:pharmacology*; D006651:Histocytochemistry; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D013211:Staphylococcus aureus / Q000737:chemistry / Q000472:pathogenicity; D013297:Streptococcus pyogenes / Q000737:chemistry / Q000472:pathogenicity
chemical_list: D001426:Bacterial Proteins; D017077:Culture Media, Conditioned; D004167:Diphtheria Toxin