title: [Influence of gastric bypass surgery on hepatic gluconeogenesis in type 2 diabetic Goto Kakizaki rats].
abstract: OBJECTIVE
  To investigate the influence and significance of gastric bypass surgery on hepatic gluconeogenesis in type 2 diabetic Goto Kakizaki(GK) rats.
  
  
  METHODS
  Forty GK rats were randomly divided into Roux-en-Y gastric bypass group(group A) and sham operation group(group B). Differences in glucose tolerance experiment(OGTT) at preoperative and postoperative 1, 2 and 4 weeks were compared and weight was recorded. Glycated hemoglobin levels were measured preoperatively and 4 weeks postoperatively. The animals were sacrificed 4 weeks after surgery and liver tissues were harvested to detect the relative expression of mRNA and protein of glucose 6 phosphatase(G-6-P) and phosphoenol pyruvate kinase(PEPCK) with RT-PCR and Western blot.
  
  
  RESULTS
  Fasting blood glucose levels were 6.5, 4.9, and 4.7 mmol/L in group A, and were 10.3, 10.4, and 12.5 mmol/L in group B, and the differences between two groups were statistically significant(P<0.05). The blood glucose level at 2 h after stomach lavage were 8.3, 6.4 and 5.5 mmol/L in group A, and were 21.4, 23.8 and 24.7 mmol/L in group B at postoperative 1, 2, 4 weeks, and the differences between two groups were statistically significant(P<0.05). The glycosylated hemoglobin at postoperative 4 weeks was(6.8±1.0)%, significantly lower than that in group B[(7.9±0.8)%, P<0.05]. Hepatic G-6-P and PEPCK mRNA relative expression at postoperative 4 weeks was reduced by 21.0% and 25.9% respectively as compared to group B, and the protein expression reduced as well. Immunohistochemistry showed that hepatic glycogen sedimentary in group A increased significantly.
  
  
  CONCLUSIONS
  The relative mRNA and protein level of key enzymes of hepatic gluconeogenesis are significantly decreased after Roux-en-Y gastric bypass surgery and hepatic gluconeogenesis is reduced, which may be a potential mechanism of the decrease of blood glucose.
authors.affiliation: Center of General Surgery, General Hospital of Chengdu Military Command, Chengdu 610083, China.
authors.forename: Song
authors.initials: S
authors.lastname: Wu
authors.forename: Jun-cheng
authors.initials: JC
authors.lastname: Tang
authors.forename: Hong
authors.initials: H
authors.lastname: Zou
authors.forename: Hong-tao
authors.initials: HT
authors.lastname: Yan
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.forename: Dong-xuan
authors.initials: DX
authors.lastname: Li
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Tang
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000601:surgery; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism* / Q000601:surgery; D015390:Gastric Bypass; D005943:Gluconeogenesis; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D006442:Glycated Hemoglobin / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008297:Male; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism; D051381:Rats
chemical_list: D001786:Blood Glucose; D005952:Glucose-6-Phosphatase; D006442:Glycated Hemoglobin; D047908:Intracellular Signaling Peptides and Proteins; D010729:Phosphoenolpyruvate Carboxykinase (GTP); C582554:Pck1 protein, rat