title: Novel cancerization marker, TP53, and its role in distinguishing normal tissue adjacent to cancerous tissue from normal tissue adjacent to benign tissue.
abstract: BACKGROUND
  The histopathological and molecular heterogeneity of normal tissue adjacent to cancerous tissue (NTAC) and normal tissue adjacent to benign tissue (NTAB), and the availability of limited specimens make deciphering the mechanisms of carcinogenesis challenging. Our goal was to identify histogenetic biomarkers that could be reliably used to define a transforming fingerprint using RNA in situ hybridization.
  
  
  METHODS
  We evaluated 15 tumor-related RNA in situ hybridization biomarkers using tumor microarray and samples of seven tumor-adjacent normal tissues from 314 patients. Biomarkers were determined using comprehensive statistical methods (significance of support vector machine-based artificial intelligence and area under curve scoring of classification distribution).
  
  
  RESULTS
  TP53 was found to be a most reliable index (P <10(-7); area under curve >87%) for distinguishing NTAC from NTAB, according to the results of a significance panel (BCL10, BECN1, BRCA2, FITH, PTCH11 and TP53).
  
  
  CONCLUSIONS
  The genetic alterations in TP53 between NTAC and NTAB may provide new insight into the field of cancerization and tumor transformation.
authors.affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, 430071, PR China.
authors.forename: Guo-Yan
authors.initials: GY
authors.lastname: Liu
authors.forename: Kun-Hong
authors.initials: KH
authors.lastname: Liu
authors.forename: Yin
authors.initials: Y
authors.lastname: Li
authors.forename: Chao
authors.initials: C
authors.lastname: Pan
authors.forename: Ji-Qin
authors.initials: JQ
authors.lastname: Su
authors.forename: Hong-Feng
authors.initials: HF
authors.lastname: Liao
authors.forename: Ren-Xiang
authors.initials: RX
authors.lastname: Yv
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Yuan
authors.forename: Huan-Jing
authors.initials: HJ
authors.lastname: Zhang
authors.forename: Chi-Meng
authors.initials: CM
authors.lastname: Tzeng
authors.forename: Bing
authors.initials: B
authors.lastname: Xiong
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D002471:Cell Transformation, Neoplastic; D016158:Genes, p53; D006801:Humans; D017403:In Situ Hybridization; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
chemical_list: D014408:Biomarkers, Tumor; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53