title: Pro-angiogenic effect of human kallikrein-related peptidase 12 (KLK12) in lung endothelial cells does not depend on kinin-mediated activation of B2 receptor.
abstract: Kallikrein-12 (KLK12) may play an important role in angiogenesis modulating proangiogenic factor bioavailability and activating the kinin receptor B2 pathway. We studied whether KLK12 had an impact on angiogenesis and the activation of kinin receptor B2 results from the KLK12-dependent generation of kinins. KLK12 efficiently hydrolyzed high molecular weight kininogen, liberating a fragment containing the carboxy-terminal end of kinins. The kininogenase activity of KLK12 was poor, however, due to the cleavage resistance of the N-terminal side of the kinin sequence. A very low amount of kinins was accordingly released after in vitro incubation of high molecular weight kininogen with KLK12 and thus the proangiogenic activity of KLK12 in lung endothelial cells was not related to a kinin release.
authors.affiliation: Université François Rabelais, EA 6305, F-37032 Tours, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kryza
authors.forename: Gilles
authors.initials: G
authors.lastname: Lalmanach
authors.forename: Marion
authors.initials: M
authors.lastname: Lavergne
authors.forename: Fabien
authors.initials: F
authors.lastname: Lecaille
authors.forename: Pascale
authors.initials: P
authors.lastname: Reverdiau
authors.forename: Yves
authors.initials: Y
authors.lastname: Courty
authors.forename: Nathalie
authors.initials: N
authors.lastname: Heuzé-Vourc'h
mesh_terms: D043925:Angiogenesis Inducing Agents; D042783:Endothelial Cells / Q000201:enzymology*; D006801:Humans; D007610:Kallikreins / Q000378:metabolism*; D007705:Kinins / Q000378:metabolism*; D008168:Lung / Q000201:enzymology*; D043782:Receptor, Bradykinin B2 / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D043925:Angiogenesis Inducing Agents; D007705:Kinins; D043782:Receptor, Bradykinin B2; C497170:KLK12 protein, human; D007610:Kallikreins