title: N-Acyl taurines trigger insulin secretion by increasing calcium flux in pancreatic β-cells.
abstract: Pancreatic β-cells secrete insulin in response to various stimuli to control blood glucose levels. This insulin release is the result of a complex interplay between signaling, membrane potential and intracellular calcium levels. Various nutritional and hormonal factors are involved in regulating this process. N-Acyl taurines are a group of fatty acids which are amidated (or conjugated) to taurine and little is known about their physiological functions. In this study, treatment of pancreatic β-cell lines (HIT-T15) and rat islet cell lines (INS-1) with N-acyl taurines (N-arachidonoyl taurine and N-oleoyl taurine), induced a high frequency of calcium oscillations in these cells. Treatment with N-arachidonoyl taurine and N-oleoyl taurine also resulted in a significant increase in insulin secretion from pancreatic β-cell lines as determined by insulin release assay and immunofluorescence (p<0.05). Our data also show that the transient receptor potential vanilloid 1 (TRPV1) channel is involved in insulin secretion in response to N-arachidonoyl taurine and N-oleoyl taurine treatment. However our data also suggest that receptors other than TRPV1 are involved in the insulin secretion response to treatment with N-oleoyl taurine.
authors.affiliation: Stockholm University, Department of Genetics, Microbiology and Toxicology, Svante Arrhenius väg 20C, 106 91 Stockholm, Sweden.
authors.forename: Dominik P
authors.initials: DP
authors.lastname: Waluk
authors.forename: Katarina
authors.initials: K
authors.lastname: Vielfort
authors.forename: Sepide
authors.initials: S
authors.lastname: Derakhshan
authors.forename: Helena
authors.initials: H
authors.lastname: Aro
authors.forename: Mary C
authors.initials: MC
authors.lastname: Hunt
mesh_terms: D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D006224:Cricetinae; D003593:Cytoplasm / Q000378:metabolism; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism; D009829:Oleic Acids / Q000494:pharmacology*; D051381:Rats; D050916:TRPV Cation Channels / Q000378:metabolism; D013654:Taurine / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D001095:Arachidonic Acids; D007328:Insulin; C581016:N-oleoyl taurine; D009829:Oleic Acids; D050916:TRPV Cation Channels; C529118:arachidonoyltaurine; D013654:Taurine; D002118:Calcium