title: The cytological observation of immune adherence of porcine erythrocyte.
abstract: The immune adherence (IA) between the porcine erythrocytes and the opsonized Escherichia coli carried green fluorescent protein gene (GFP-E.coli) were detected by the fluorescence microscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) with an attempt to verify the existence of IA between the porcine erythrocytes and complemented-opsonized microbes. Under fluorescence microscopy, GFP-E.coli opsonized by fresh rabbit serum complement adhered to the erythrocytes and could not be detached by PBS washing, and no IA was observed between the erythrocytes and nonopsonized GFP-E.coli after co-incubation. SEM and TEM also revealed the existence of IA between the serum complement-opsonized GFP-E.coli membrane and the erythrocyte membrane. The partial complement receptor type 1 (CR1)-like gene from porcine was generated by RT-PCR and rapid amplification of cDNA 3' end (3' RACE) (157bp and 578bp), both of which have high similarity with published mammal's CR1 gene. The sequences were spliced based on homology comparison and submitted to GenBank (GenBank Accession No. JX033989). These results indicated that the porcine erythrocytes were able to bind to the opsonized microorganisms. Furthermore, the sequencing results confirmed that the CR1-like gene exists in porcine.
authors.affiliation: Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, P R China.
authors.forename: Yao-Gui
authors.initials: YG
authors.lastname: Sun
authors.forename: Wei
authors.initials: W
authors.lastname: Yin
authors.forename: Xin-Feng
authors.initials: XF
authors.lastname: Fan
authors.forename: Kuo-Hai
authors.initials: KH
authors.lastname: Fan
authors.forename: Jun-Bing
authors.initials: JB
authors.lastname: Jiang
authors.forename: Hong-Quan
authors.initials: HQ
authors.lastname: Li
mesh_terms: D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion; D003165:Complement System Proteins / Q000276:immunology / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000276:immunology; D004912:Erythrocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D004926:Escherichia coli / Q000276:immunology / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007104:Immune Adherence Reaction; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009895:Opsonin Proteins / Q000378:metabolism; D011817:Rabbits; D011951:Receptors, Complement / Q000235:genetics / Q000378:metabolism; D017385:Sequence Homology; D013552:Swine; D014231:Tribolium / Q000235:genetics
chemical_list: D009895:Opsonin Proteins; D011951:Receptors, Complement; D049452:Green Fluorescent Proteins; D003165:Complement System Proteins