title: DARC (Duffy) and BCAM (Lutheran) reduced expression in thyroid cancer.
abstract: Duffy or DARC (Duffy Antigen Receptor for Chemokines) is a glycosylated membrane protein that selectively binds angiogenic chemokines. Previous in vivo and in vitro studies of DARC function in cancer have associated DARC over expression with better prognosis, decreased metastatic potential, and inhibition of tumor-associated neovascularization. Another carcinogenesis-associated antigen is Lutheran or BCAM (basal cell adhesion molecule), a surface glycoprotein that acts as a receptor for the extracellular matrix protein, laminin. BCAM is a protein related to tumor progression; and, its over expression is associated with skin, ovarian and pancreatic cancers. We explored DARC and BCAM functions and investigated whether or not their expressions were altered in thyroid cancer. The expression of DARC and BCAM were evaluated by quantitative real-time PCR (qPCR) in a set of 18 normal thyroid tissues (NT), 15 follicular adenomas (FTA), 17 follicular carcinomas (FTC), and 122 papillary thyroid carcinomas (PTC), including 78 classical (CVPTC) and 44 follicular variant (FVPTC). RNA was isolated, reverse transcribed to cDNA, and used in qPCR reactions containing SYBR Green. The relative expression value was calculated using ribosomal protein S8 as an internal control. When we compared benign (NT and FTA) versus malignant samples (FTC, CVPTC and FVPTC) we observed a significant decrease of DARC and BCAM relative expression in malignant cases. Additionally, we correlated clinic-pathological features (tumor size, presence of metastasis, presence of lymphocyte infiltrate) with DARC and BCAM expression. We found a diminished expression of DARC in PTC samples, which was correlated with tumor size and presence of a lymphocyte infiltrate. We, also, found a correlation between decreased BCAM expression and tumor size or presence of metastasis. DARC and BCAM expression was associated with pathogenesis of thyroid carcinoma and correlated with clinical-pathological features.
authors.affiliation: Colsan - Associação Beneficente de Coleta de Sangue, Avenida Jandira 1260, Indianópolis, São Paulo, SP, CEP 04080-006, Brazil. flalatini@hotmail.com
authors.forename: Flavia Roche Moreira
authors.initials: FR
authors.lastname: Latini
authors.forename: André Uchimura
authors.initials: AU
authors.lastname: Bastos
authors.forename: Carine Prisco
authors.initials: CP
authors.lastname: Arnoni
authors.forename: Janaína Guilhem
authors.initials: JG
authors.lastname: Muniz
authors.forename: Rosangela Medeiros
authors.initials: RM
authors.lastname: Person
authors.forename: Wilson
authors.initials: W
authors.lastname: Baleotti
authors.forename: José Augusto
authors.initials: JA
authors.lastname: Barreto
authors.forename: Lilian
authors.initials: L
authors.lastname: Castilho
authors.forename: Janete Maria
authors.initials: JM
authors.lastname: Cerutti
mesh_terms: D002277:Carcinoma / Q000235:genetics / Q000473:pathology; D002291:Carcinoma, Papillary; D015815:Cell Adhesion Molecules / Q000235:genetics*; D004375:Duffy Blood-Group System / Q000235:genetics*; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008188:Lutheran Blood-Group System / Q000235:genetics*; D009362:Neoplasm Metastasis; D011956:Receptors, Cell Surface / Q000235:genetics*; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology; D047368:Tumor Burden
chemical_list: C473745:ACKR1 protein, human; C090228:BCAM protein, human; D015815:Cell Adhesion Molecules; D004375:Duffy Blood-Group System; D008188:Lutheran Blood-Group System; D011956:Receptors, Cell Surface