title: HPLC analysis and cytotoxic activity of Vernonia cinerea.
abstract: The extracts of five Cambodian medicinal plants (Aganosma marginata, Dracaena cambodiana, Harrisonia perforata, Hymenodictyon excelsum and Vernonia cinerea) were evaluated in vitro for their cytotoxic activity against HT29 colon adenocarcinoma cells and HepG2 hepatoma cells, using the MTT assay. Among these five plants, Vernonia cinerea displayed potent cytotoxicity. One main sesquiterpene lactone, 8alpha-tigloyloxy-hirsutinolide-13-O-acetate was isolated from the whole plant of V. cinerea. This compound was active against both cancer cell lines (IC50 = 3.50 microM for HT29 and IC50 = 4.27 microM for HepG2). To quantify this compound in the plant, an analytical high-performance liquid chromatography (HPLC) method was developed and validated.
authors.affiliation: Laboratory of Pharmacognosy and Ethnopharmacology, UMR-MD3, Faculty of Pharmacy, Aix-Marseille University, 13385 Marseille cedex 5, France.
authors.forename: Mom
authors.initials: M
authors.lastname: Khay
authors.forename: Phirom
authors.initials: P
authors.lastname: Toeng
authors.forename: Valérie
authors.initials: V
authors.lastname: Mahiou-Leddet
authors.forename: Fathi
authors.initials: F
authors.lastname: Mabrouki
authors.forename: Kim
authors.initials: K
authors.lastname: Sothea
authors.forename: Evelyne
authors.initials: E
authors.lastname: Ollivier
authors.forename: Riad
authors.initials: R
authors.lastname: Elias
authors.forename: Sok-Siya
authors.initials: SS
authors.lastname: Bun
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis* / Q000494:pharmacology; D002160:Cambodia; D002851:Chromatography, High Pressure Liquid; D004354:Drug Screening Assays, Antitumor; D019073:HT29 Cells; D006801:Humans; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol; D008752:Methylene Chloride; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012997:Solvents; D013778:Tetrazolium Salts; D013844:Thiazoles; D035741:Vernonia / Q000737:chemistry*; D014867:Water
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D007202:Indicators and Reagents; D012717:Sesquiterpenes; D012997:Solvents; D013778:Tetrazolium Salts; D013844:Thiazoles; D014867:Water; D008752:Methylene Chloride; C022616:thiazolyl blue; D000432:Methanol