title: A nucleic acid probe labeled with desmethyl thiazole orange: a new type of hybridization-sensitive fluorescent oligonucleotide for live-cell RNA imaging.
abstract: A new fluorescent nucleotide with desmethyl thiazole orange dyes, D'(505), has been developed for expansion of the function of fluorescent probes for live-cell RNA imaging. The nucleoside unit of D'(505) for DNA autosynthesis was soluble in organic solvents, which made the preparation of nucleoside units and the reactions in the cycles of DNA synthesis more efficient. The dyes of D'(505)-containing oligodeoxynucleotide were protonated below pH 7 and the oligodeoxynucleotide exhibited hybridization-sensitive fluorescence emission through the control of excitonic interactions of the dyes of D'(505). The simplified procedure and effective hybridization-sensitive fluorescence emission produced multicolored hybridization-sensitive fluorescent probes, which were useful for live-cell RNA imaging. The acceptor-bleaching method gave us information on RNA in a specific cell among many living cells.
authors.affiliation: Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan. okamoto@chembio.t.u-tokyo.ac.jp
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Okamoto
authors.forename: Kaori
authors.initials: K
authors.lastname: Sugizaki
authors.forename: Mizue
authors.initials: M
authors.lastname: Yuki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yanagisawa
authors.forename: Shuji
authors.initials: S
authors.lastname: Ikeda
authors.forename: Takuma
authors.initials: T
authors.lastname: Sueoka
authors.forename: Gosuke
authors.initials: G
authors.lastname: Hayashi
authors.forename: Dan Ohtan
authors.initials: DO
authors.lastname: Wang
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry / Q000378:metabolism*; D002470:Cell Survival; D002942:Circular Dichroism; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D057054:Molecular Imaging / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015341:Nucleic Acid Probes / Q000737:chemistry / Q000378:metabolism*; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D038761:Photobleaching; D011522:Protons; D011804:Quinolines / Q000737:chemistry / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D013194:Staining and Labeling; D013942:Thymine Nucleotides / Q000737:chemistry / Q000378:metabolism*
chemical_list: D052160:Benzothiazoles; C581137:D'(505) nucleotide; D005456:Fluorescent Dyes; D015341:Nucleic Acid Probes; D009841:Oligonucleotides; D011522:Protons; D011804:Quinolines; D013942:Thymine Nucleotides; C051000:thiazole orange; D012313:RNA