title: Plexin-B2 regulates the proliferation and migration of neuroblasts in the postnatal and adult subventricular zone.
abstract: In the postnatal forebrain, the subventricular zone (SVZ) contains a pool of undifferentiated cells, which proliferate and migrate along the rostral migratory stream (RMS) to the olfactory bulb and differentiate into granule cells and periglomerular cells. Plexin-B2 is a semaphorin receptor previously known to act on neuronal proliferation in the embryonic brain and neuronal migration in the cerebellum. We show here that, in the postnatal and adult CNS, Plexin-B2 is expressed in the subventricular zone lining the telencephalic ventricles and in the rostral migratory stream. We analyzed Plxnb2(-/-) mice and found that there is a marked reduction in the proliferation of SVZ cells in the mutant. Plexin-B2 expression is downregulated in the olfactory bulb as interneurons initiate radial migration. BrdU labeling and GFP electroporation into postnatal SVZ, in addition to time-lapse videomicroscopy, revealed that neuroblasts deficient for Plexin-B2 migrate faster than control ones and leave the RMS more rapidly. Overall, these results show that Plexin-B2 plays a role in postnatal neurogenesis and in the migration of SVZ-derived neuroblasts.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, UMR S968, France.
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Saha
authors.forename: Athéna R
authors.initials: AR
authors.lastname: Ypsilanti
authors.forename: Camille
authors.initials: C
authors.lastname: Boutin
authors.forename: Harold
authors.initials: H
authors.lastname: Cremer
authors.forename: Alain
authors.initials: A
authors.lastname: Chédotal
mesh_terms: D000818:Animals; D000963:Antimetabolites; D016380:Brain Tissue Transplantation; D001973:Bromodeoxyuridine; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D018274:Electroporation; D005260:Female; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000502:physiology; D017228:Hepatocyte Growth Factor / Q000502:physiology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000502:physiology*; D055495:Neurogenesis / Q000235:genetics / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D009830:Olfactory Bulb / Q000502:physiology; D016548:Prosencephalon / Q000166:cytology / Q000502:physiology*
chemical_list: D000963:Antimetabolites; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009419:Nerve Tissue Proteins; C519231:Plxnb2 protein, mouse; D017228:Hepatocyte Growth Factor; D001973:Bromodeoxyuridine