title: Polymorphism of CD36 gene, carbohydrate metabolism and plasma CD36 concentration in obese children. A preliminary study.
abstract: BACKGROUND
  CD36 may play an important role in removal of oxidized LDLs from plasma, protein glycation, the pathogenesis of insulin resistance, type 2 diabetes, and diabetic micro- and macroangiopathy. Some reports have pointed to decreased expression of macrophages in association with mutations of the CD36 gene in hyperglycemic and obese subjects. The aim of the study was to search for an association between CD36 gene polymorphism and carbohydrate metabolism disturbances or variability of plasma soluble CD36 concentrations in obese children.
  
  
  METHODS
  The study included 60 children aged 10 to 15 years: 30 with (study group) and 30 without (control group) obesity. Each patient's glycated hemoglobin, weight, height, waist and hip circumference, and systolic and diastolic blood pressure were measured, BMI, WHR and MAP were calculated, and oral glucose tolerance test was performed with glucose and insulin concentration measurements. Amplicons of exons 4-6 of CD36 were studied using DHPLC technique. The PCR products with alterations were bidirectionally sequenced. Plasma concentrations of human antigen CD36 was measured using a commercially available enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  We found two intronic alterations: IVS3-6 T/C (rs3173798) and IVS4-10 G/A (rs3211892), one nonsynonymous substitution: G367A (Glu123Lys, rs183461468) in exon 5 and two synonymous transitions in exon 6: G573A (Pro191Pro, rs5956) and A591T (Thr197Thr, rs141680676). There were no significant differences in any biochemical or morphometric parameters between genotype groups.
  
  
  CONCLUSIONS
  The polymorphisms of the studied fragment of CD36 are not associated with carbohydrate metabolism disturbances or the variability of plasma soluble CD36 concentrations in obese children, but further research is necessary to assess their functional implications.
authors.affiliation: Department of Biochemistry and Medical Chemistry Pomeranian Medical University, 70-111 Szczecin, Poland. carmon12@gmail.com
authors.forename: Monika E
authors.initials: ME
authors.lastname: Rać
authors.forename: Beata
authors.initials: B
authors.lastname: Krupa
authors.forename: Barbara
authors.initials: B
authors.lastname: Garanty-Bogacka
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Syrenicz
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Safranow
authors.forename: Violetta
authors.initials: V
authors.lastname: Dziedziejko
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Kurzawski
authors.forename: Maria
authors.initials: M
authors.lastname: Olszewska
authors.forename: Michał
authors.initials: M
authors.lastname: Rać
authors.forename: Dariusz
authors.initials: D
authors.lastname: Chlubek
mesh_terms: D000293:Adolescent; D018955:CD36 Antigens / Q000097:blood* / Q000235:genetics*; D050260:Carbohydrate Metabolism / Q000235:genetics*; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D009765:Obesity / Q000097:blood / Q000235:genetics* / Q000378:metabolism*; D011110:Polymorphism, Genetic; D012016:Reference Values
chemical_list: D018955:CD36 Antigens