title: Polypharmacy (herbal and synthetic drug combination): a novel approach in the treatment of type-2 diabetes and its complications in rats.
abstract: The aim of present study was to evaluate the effect of aqueous leaf extract of Annona squamosa with Glipizide in a high fat diet and streptozocin-induced type-2 diabetes. Nine groups (n = 6) of male Sprague-Dawley rats were used for the study, with. Basal blood glucose, urine volume, and body weights were measured and the rate were kept on a high fat diet. After 15 days, streptozocin in sub-diabetic dose (35 mg/kg) was administered to the animals to induce diabetes. With 1 week of consistent hyperglycemia, treatment was initiated. Aqueous extract of Annona squamosa was administered orally at 350 mg/kg body weight alone and in combination with reduced and reducing dose combinations of Glipizide. Blood glucose, body weight, urine volume were measured every 10th day. The elevated blood-glucose level in diabetic rats was controlled better with combination therapy compared with the synthetic drug alone or the herbal stand-alone drug. All the results were statistically significant (P < 0.001). A combination of Annona squamosa along with Glipizide may be helpful in dose reduction of Glipizide up to 50%, reducing the risk of the onset of insulin therapy.
authors.affiliation: Siddhartha Institute of Pharmacy, Dehra Dun, 248001, Uttarakhand, India.
authors.forename: Rupinder
authors.initials: R
authors.lastname: Kaur
authors.forename: Muhammad
authors.initials: M
authors.lastname: Afzal
authors.forename: Imran
authors.initials: I
authors.lastname: Kazmi
authors.forename: Iqbal
authors.initials: I
authors.lastname: Ahamd
authors.forename: Zubair
authors.initials: Z
authors.lastname: Ahmed
authors.forename: Babar
authors.initials: B
authors.lastname: Ali
authors.forename: Sayeed
authors.initials: S
authors.lastname: Ahmad
authors.forename: Firoz
authors.initials: F
authors.lastname: Anwar
mesh_terms: D000284:Administration, Oral; D000818:Animals; D031041:Annona / Q000737:chemistry*; D001522:Behavior, Animal / Q000187:drug effects; D001786:Blood Glucose / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D048909:Diabetes Complications / Q000188:drug therapy; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy* / Q000652:urine; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000150:complications / Q000188:drug therapy* / Q000652:urine; D004359:Drug Therapy, Combination; D005913:Glipizide / Q000008:administration & dosage / Q000627:therapeutic use*; D005978:Glutathione / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D007328:Insulin / Q000097:blood; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; D010936:Plant Extracts; D002374:Catalase; D005978:Glutathione; D005913:Glipizide