title: Evaluation of the chondroprotective effects of glucosamine and fish collagen peptide on a rabbit ACLT model using serum biomarkers.
abstract: The aim of this study was to investigate the correlations of severity of osteoarthritis (OA) and serum biomarkers including keratan sulfate (KS), hyaluronic acid (HA) and chondroitin sulfate (CS) 846 epitope. We also investigated the effect of glucosamine and fish collagen peptide (FCP) on OA. OA was induced in 12 rabbits (12 weeks of age) by anterior cruciate ligament transection (ACLT). After the surgery, the rabbits were orally administered FCP (F group), glucosamine (G group) or FCP and glucosamine (FG group) for 4 weeks. The control group was provided water ad libitum (C group). Blood was collected before surgery (pre-ACLT) and before euthanasia (post-ACLT) for serum marker measurement. Biomarker levels were measured by using commercial kits. We evaluated OA severity both macroscopically and histologically. Macroscopic evaluation showed mildly eroded condylar surfaces in the C group. Histological findings were significantly different from the FG and other groups. There were no significant differences between each group at post-ACLT in terms of serum KS, HA and CS 846. Histological assessment and serum biomarker measurements performed at post-ACLT showed a significant correlation between HA concentration and OA severity. Variations in the CS 846 concentration at pre-ACLT and post-ACLT were significantly correlated with OA severity. Administration of glucosamine and FCP had chondroprotective effects in the ACLT model. Serum biomarker concentrations were significantly correlated with cartilage injury. Serum biomarker measurement would be useful for monitoring articular cartilage damage in the clinical setting.
authors.affiliation: Department of Veterinary Clinical Medicine, Tottori University, 4-101 Koyama-Minami, Tottori, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ohnishi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Osaki
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Matahira
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tsuka
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Imagawa
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Okamoto
authors.forename: Saburo
authors.initials: S
authors.lastname: Minami
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000473:pathology* / Q000601:surgery*; D015415:Biomarkers / Q000097:blood; D002358:Cartilage, Articular / Q000473:pathology*; D002809:Chondroitin Sulfates / Q000097:blood; D003094:Collagen / Q000494:pharmacology*; D004195:Disease Models, Animal; D005944:Glucosamine / Q000494:pharmacology*; D006651:Histocytochemistry / Q000662:veterinary; D006820:Hyaluronic Acid / Q000097:blood; D007632:Keratan Sulfate / Q000097:blood; D010003:Osteoarthritis / Q000097:blood / Q000188:drug therapy* / Q000473:pathology / Q000601:surgery; D011817:Rabbits; D018709:Statistics, Nonparametric
chemical_list: D015415:Biomarkers; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; D003094:Collagen; D007632:Keratan Sulfate; D005944:Glucosamine