title: [Clinical significance of telomerase activity detection of myeloid element in immune related hematocytopenia].
abstract: OBJECTIVE
  To observe the telomerase activity (TA) in bone marrow mononuclear cell (BMMNC) at pre-therapy and post-therapy in patients with immune related hematocytopenia and immune related pancytopenia (IRH/IRP) so as to explore the relationship between TA and disease process.
  
  
  METHODS
  TA in BMMNC of 108 IRH/IRP patients was detected with telomeric repeat amplification protocol (TRAP)-PCR-ELISA. The expressions of anti-human immunoglobulin IgG, FcγII receptor (FcγIIR), mannose receptor (MR) and interleukin 17A (IL-17A) located on membrane surface were observed by immunofluorescence (IF). A total of 30 iron deficiency anemia patients were selected as case control.
  
  
  RESULTS
  The TA of 108 IRH/IRP patients were 0.251 ± 0.021. And it increased as compared with the control group of 0.062 ± 0.031 at pre-therapy (P < 0.01). The HLA-B27(+) patients among them was 0.270 ± 0.020 while HLA-B27(-) patients 0.181 ± 0.013 (P < 0.05). And HLA-B27(+)IgG elevated IRP was 0.341 ± 0.016 and HLA-B27(-)IgG elevated 0.183 ± 0.010, the difference of TA was significant between two groups (P < 0.01). IgG antibody positive immature hemocyte could be observed in marrow of IRH/IRP patients, and FcγIIR was expressed highly on those activated macrophage (Mφ) in bone marrow. Both IL-17A and MR were expressed on activated dendritic cells and Mφ in marrow of HLA-B27(+) patients. After the therapy of glucocorticoids associated with cyclosporine A and other drugs, the patient's hemogram recovered and the telomerase in BMMNC were re-devitalized.
  
  
  CONCLUSIONS
  The TA of the BMMNC in marrow of IRH/IRP patients is increased, and it shows a close relation between apoptotic degree of hematopoietic cell and the activation of immunocytes. In IRH/IRP, synchronism and equilibrium exist between BMMNC apoptosis and TA.
authors.affiliation: Department of Tumor Research & Treatment Center, China. sunlifei_2007@163.com
authors.forename: Li-fei
authors.initials: LF
authors.lastname: Sun
authors.forename: Bing
authors.initials: B
authors.lastname: Han
authors.forename: Qiang-qiang
authors.initials: QQ
authors.lastname: Wu
authors.forename: Chang-wei
authors.initials: CW
authors.lastname: Liu
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Xiang-shan
authors.initials: XS
authors.lastname: Ma
authors.forename: Hong-feng
authors.initials: HF
authors.lastname: Hao
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000888:Antibodies, Anti-Idiotypic / Q000097:blood; D001853:Bone Marrow / Q000378:metabolism; D001854:Bone Marrow Cells / Q000201:enzymology*; D003713:Dendritic Cells / Q000378:metabolism; D005260:Female; D015796:HLA-B27 Antigen / Q000378:metabolism; D006801:Humans; D020381:Interleukin-17 / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010198:Pancytopenia / Q000209:etiology / Q000276:immunology / Q000378:metabolism*; D017452:Receptors, IgG / Q000378:metabolism; D019098:Telomerase / Q000378:metabolism*; D055815:Young Adult
chemical_list: D000888:Antibodies, Anti-Idiotypic; C445323:FCGR2A protein, human; D015796:HLA-B27 Antigen; D020381:Interleukin-17; D017452:Receptors, IgG; D019098:Telomerase