title: No evidence for involvement of the toll-like receptor (TLR) 4 gene Asp299Gly and Thr399Ile polymorphisms in susceptibility to primary gouty arthritis.
abstract: Previous studies demonstrated that toll-like receptor (TLR) 4 was involved in the development of autoinflammatory disease including gouty arthritis (GA). TLR4 functional gene Asp299Gly and Thr399Ile polymorphisms play a role in some autoinflammatory disease susceptibility. We undertook this study to analyze the association between the genetic polymorphisms within TLR4 gene and the susceptibility to GA in Chinese Han people. Two functional variants, Asp299Gly and Thr399Ile, in the TLR4 gene were genotyped using 5' exonuclease TaqMan technology from 218 male GA patients and 226 ethnically matched controls. None polymorphisms of Asp299Gly and Thr399Ile were detected in all GA cases and controls, which indicates that there is no evidence for involvement of the TLR4 gene Asp299Gly and Thr399Ile polymorphisms in susceptibility to primary GA in the Chinese Han population. Further studies with extended single nucleotide polymorphisms should be performed.
authors.affiliation: Department of Rheumatology and Hematology, Institute of Rheumatology and Immunology, The Affiliated Hospital of North Sichuan Medical College, 63 Wenhua Road, Nanchong, 637000, Sichuan, China, qingyufengqq@163.com.
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Qing
authors.forename: Jing-Guo
authors.initials: JG
authors.lastname: Zhou
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Wen-Guang
authors.initials: WG
authors.lastname: Xie
authors.forename: Cui-Ping
authors.initials: CP
authors.lastname: Huang
authors.forename: Sheng-Ping
authors.initials: SP
authors.lastname: Zeng
authors.forename: Ling
authors.initials: L
authors.lastname: Yin
mesh_terms: D000328:Adult; D000368:Aged; D015210:Arthritis, Gouty / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D002681:China; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D051197:Toll-Like Receptor 4 / Q000235:genetics*
chemical_list: C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4