title: Production and antimicrobial activity of 3-hydroxypropionaldehyde from Bacillus subtilis strain CU12.
abstract: Bacillus subtilis strains are known to produce a vast array of antimicrobial compounds. However, some compounds remain to be identified. Disk assays performed in vitro with Bacillus subtilis CU12 showed a significant reduction in mycelial growth of Alternaria solani, Botrytis cinerea, Fusarium sambucinum, and Pythium sulcatum. Crude B. subtilis culture filtrates were subsequently extracted with ethyl acetate and butanol. A bioassay guided purification procedure revealed the presence of one major antifungal compound in the butanol extract. Purification of the compound was performed using a reverse-phase C18 solid phase extraction (SPE) cartridge and flash column chromatography. NMR data showed that the main antimicrobial compound was a cyclic dimer of 3-hydroxypropionaldehyde (HPA). This study demonstrated the antimicrobial activity of B. subtilis strain CU12 against phytopathogenic microorganisms is mediated at least in part by the production of HPA. It also suggests that this B. subtilis strain could be effective at controlling pathogens through protection of its ecological niche by antibiosis.
authors.affiliation: Department of Chemistry, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario, Canada, K1S 5B6.
authors.forename: C
authors.initials: C
authors.lastname: Wise
authors.forename: L
authors.initials: L
authors.lastname: Novitsky
authors.forename: A
authors.initials: A
authors.lastname: Tsopmo
authors.forename: T J
authors.initials: TJ
authors.lastname: Avis
mesh_terms: D000528:Alternaria / Q000187:drug effects; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D001412:Bacillus subtilis / Q000737:chemistry / Q000378:metabolism*; D020171:Botrytis / Q000187:drug effects; D056148:Chromatography, Reverse-Phase; D019281:Dimerization; D005670:Fusarium / Q000187:drug effects; D005985:Glyceraldehyde / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D011407:Propane / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D011775:Pythium / Q000187:drug effects; D052616:Solid Phase Extraction
chemical_list: D000890:Anti-Infective Agents; C047158:3-hydroxypropionaldehyde; D005985:Glyceraldehyde; D011407:Propane