title: Effect of Wolbachia on insecticide susceptibility in lines of Aedes aegypti.
abstract: Two stable infections of Wolbachia pipientis, wMelPop and wMel, now established in Aedes aegypti, are being used in a biocontrol program to suppress the transmission of dengue. Any effects of Wolbachia infection on insecticide resistance of mosquitoes may undermine the success of this program. Bioassays of Ae. aegypti were conducted to test for differences in response to insecticides between Wolbachia infected (wMelPop, wMel) and uninfected lines. Insecticides screened were bifenthrin, the pyrethroid commonly used for adult knockdown, as well as larvicides: Bacillus thuringiensis var. israelensis, the organophosphate, temephos and the insect growth regulator, s-methoprene. While differences in response between lines were detected for some insecticides, no obvious or consistent effects related to presence of Wolbachia infection were observed. Spreading Wolbachia infections are, therefore, unlikely to affect the efficacy of traditional chemical control of mosquito outbreaks.
authors.affiliation: Department of Genetics, Bio21 Institute, The University of Melbourne, Victoria 3010, Australia. nancye@unimelb.edu.au
authors.forename: N M
authors.initials: NM
authors.lastname: Endersby
authors.forename: A A
authors.initials: AA
authors.lastname: Hoffmann
mesh_terms: D000330:Aedes / Q000187:drug effects* / Q000382:microbiology*; D000818:Animals; D001413:Bacillus thuringiensis / Q000472:pathogenicity; D003715:Dengue / Q000517:prevention & control; D007305:Insecticide Resistance / Q000502:physiology*; D007306:Insecticides / Q000633:toxicity*; D008726:Methoprene / Q000633:toxicity; D010572:Pest Control, Biological / Q000379:methods*; D011722:Pyrethrins / Q000633:toxicity; D000002:Temefos / Q000633:toxicity; D020577:Wolbachia / Q000502:physiology*
chemical_list: D007306:Insecticides; D011722:Pyrethrins; C099952:bifenthrin; D008726:Methoprene; D000002:Temefos