title: Carbapenem-resistant Klebsiella pneumoniae harbouring blaKPC-3 and blaVIM-2 from central Italy.
abstract: The frequency of Klebsiella pneumoniae carbapenemase-producing K. pneumoniae is increasing in Italian hospitals and poses an emerging threat to the management of infections in hospitalized patients. In this study, we report a detailed molecular characterization of a K. pneumoniae subsp. pneumoniae KP1/11 isolate from the decubitus ulcer of a hospitalized patient with a serious infection. K. pneumoniae KP1/11 produces KPC-3 and VIM-2 β-lactamases. The bla(KPC-3) gene is harbored in a large plasmid in a complex structure of Tn3-based transposon, Tn4401a. The chromosomal DNA of K. pneumoniae harbored also 2 class 1 integrons with different variable regions: 1) orfD-aacA8; 2) aacA29-bla(VIM-2).
authors.affiliation: Dipartimento di Scienze Cliniche Applicate e Biotecnologiche, Università degli Studi dell'Aquila, L'Aquila, Italy. perilli@univaq.it
authors.forename: Mariagrazia
authors.initials: M
authors.lastname: Perilli
authors.forename: Carlo
authors.initials: C
authors.lastname: Bottoni
authors.forename: Alessandro
authors.initials: A
authors.lastname: Grimaldi
authors.forename: Bernardetta
authors.initials: B
authors.lastname: Segatore
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Celenza
authors.forename: Maurizio
authors.initials: M
authors.lastname: Mariani
authors.forename: Pierangelo
authors.initials: P
authors.lastname: Bellio
authors.forename: Patrizia
authors.initials: P
authors.lastname: Frascaria
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Amicosante
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D001483:Base Sequence; D015780:Carbapenems / Q000494:pharmacology; D006801:Humans; D007558:Italy; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; C425108:beta-lactamase bla(vim-2); D001618:beta-Lactamases; C495220:beta-lactamase KPC-3, Klebsiella pneumoniae