title: Mushroom body-preferential expression of proteins/genes involved in endoplasmic reticulum Ca(2+)-transport in the worker honeybee (Apis mellifera L.) brain.
abstract: To identify the molecular characteristics specific to the mushroom body (MB, a higher processing centre) neurones in the honeybee brain, we previously used proteomics to identify proteins that are preferentially expressed in these MBs. Here we continued our proteomic analysis to show that reticulocalbin, which is involved in endoplasmic reticulum (ER) Ca(2+) transport, is also preferentially expressed in the MBs in the honeybee brain. Gene expression analysis revealed that reticulocalbin is preferentially expressed in the large-type Kenyon cells, which are MB-intrinsic neurones. In addition, the gene for the ryanodine receptor, which is also involved in ER Ca(2+) transport, was also preferentially expressed in the large-type Kenyon cells. In contrast, the expression of three other ER-related genes, protein disulphide isomerase, sec61 and erp60, was not enriched in the MBs. These findings further support the notion that the function of ER Ca(2+)-signalling, but not the mere intracellular density of ER, is specifically enhanced in the large-type Kenyon cells in the honeybee brain.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Uno
authors.forename: T
authors.initials: T
authors.lastname: Fujiyuki
authors.forename: M
authors.initials: M
authors.lastname: Morioka
authors.forename: T
authors.initials: T
authors.lastname: Kubo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001516:Bees / Q000235:genetics / Q000502:physiology*; D001692:Biological Transport; D001921:Brain / Q000502:physiology; D002118:Calcium / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D037282:Calreticulin / Q000235:genetics / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D024521:Mushroom Bodies / Q000378:metabolism / Q000502:physiology*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics* / Q000378:metabolism
chemical_list: D002135:Calcium-Binding Proteins; D037282:Calreticulin; D019476:Insect Proteins; D019837:Ryanodine Receptor Calcium Release Channel; C078865:reticulocalbin; D017853:Phosphotransferases (Alcohol Group Acceptor); C460370:juvenile hormone diol kinase; D002118:Calcium