title: Effects of Tepary bean (Phaseolus acutifolius) protease inhibitor and semipure lectin fractions on cancer cells.
abstract: Some natural and synthetic protease inhibitors (PI), such as the Bowman-Birk PI from soybean, have anticancer effects. We previously purified and characterized a Bowman-Birk-type PI from Tepary bean (Phaseolus acutifolius) seeds (TBPI). A semipure protein fraction containing this inhibitor, when tested its in vitro effect on transformed cells, showed a differential cytotoxic effect, as well as an increase in cell attachment to culture dishes. In this article we report that lectins were responsible for the cytotoxic effect previously observed, exhibiting a differential, antiproliferative effect on nontransformed cells and on different lineages of cancer cells. Although the purified TBPI lacked cytotoxicity, it was found to be responsible for the increase in cell adhesion, decreasing culture dishes' extracellular matrix degradation, leading to a decrease of the in vitro cell invasion capacity. This effect coincided with the suppression of Matrix Metalloproteinase-9 activity. These results indicate that Tepary bean seeds contain at least 2 different groups of bioactive proteins with distinct effects on cancer cells.
authors.affiliation: Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Querétaro, México. tggasca@gmail.com
authors.forename: Teresa
authors.initials: T
authors.lastname: García-Gasca
authors.forename: Marlen
authors.initials: M
authors.lastname: García-Cruz
authors.forename: Elisa
authors.initials: E
authors.lastname: Hernandez-Rivera
authors.forename: Josue
authors.initials: J
authors.lastname: López-Matínez
authors.forename: Ana L
authors.initials: AL
authors.lastname: Castañeda-Cuevas
authors.forename: Lorena
authors.initials: L
authors.lastname: Yllescas-Gasca
authors.forename: Adriana J
authors.initials: AJ
authors.lastname: Rodríguez-Méndez
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Mendiola-Olaya
authors.forename: José L
authors.initials: JL
authors.lastname: Castro-Guillén
authors.forename: Alejandro
authors.initials: A
authors.lastname: Blanco-Labra
mesh_terms: D016475:3T3 Cells; D000818:Animals; D018938:Caco-2 Cells; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D061986:MCF-7 Cells; D051379:Mice; D009361:Neoplasm Invasiveness / Q000517:prevention & control; D027805:Phaseolus / Q000737:chemistry*; D011480:Protease Inhibitors / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry
chemical_list: D037102:Lectins; D011480:Protease Inhibitors