title: Genetic characterization of Toxoplasma gondii in wildlife from Alabama, USA.
abstract: The genetic diversity of Toxoplasma gondii circulating in wildlife is of interest to understand the transmission of this parasite in the environment. In the present study, we genetically characterized five T. gondii isolates from different wild animals including two isolates from a bobcat (Lynx rufus), one from a red-shouldered hawk (Buteo lineatus), one from a white-tailed deer (Odocoileus virginianus), and one from a bald eagle (Haliaeetus leucocephalus). Genotyping of these samples using 11 PCR-restriction fragment length polymorphism markers (SAG1, 5'- and 3'-SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico) revealed two types, including type I (ToxoDB#10) and type 12 (ToxoDB#5). This is the first report of genetic characterization of T. gondii strains in wildlife from Alabama and from a red-shouldered hawk.
authors.affiliation: Department of Microbiology and Parasitology, Anhui Provincial Laboratory of Microbiology and Parasitology; Anhui Key Laboratory of Zoonoses, Anhui Medical University, Hefei, 230022, People's Republic of China. lilyyu33@gmail.com
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.forename: Jilong
authors.initials: J
authors.lastname: Shen
authors.forename: Chunlei
authors.initials: C
authors.lastname: Su
authors.forename: Christine A
authors.initials: CA
authors.lastname: Sundermann
mesh_terms: D000407:Alabama; D000818:Animals; D000835:Animals, Wild; D016054:DNA, Protozoan / Q000235:genetics; D020307:Eagles / Q000469:parasitology; D046093:Falconiformes / Q000469:parasitology; D014644:Genetic Variation; D005838:Genotype; D046008:Lynx / Q000469:parasitology; D012150:Polymorphism, Restriction Fragment Length; D014122:Toxoplasma / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D014124:Toxoplasmosis, Animal / Q000469:parasitology*
chemical_list: D016054:DNA, Protozoan