title: Chemoenzymatic synthesis of two new halogenated coumarin glycosides as potential antifungal agents.
abstract: Two new potential antifungal coumarin glycosides, 6-chlorocoumarin 7-O-beta-D-glucopyranoside (1) and 7-hydroxy-4-trifluoromethyl-coumarin 5-O-beta-D-glucopyranoside (2), were synthesized via enzyme-mediated glycosylation of the respective aglycone, 6-chloro-7-hydroxycoumarin and 5,7-dihydroxy-4-trifluoromethylcoumarin, using transgenic hairy roots of Polygonum multiflorum. Instead of application of the isolated enzyme and exogenous sugar donors, hairy roots of P. multiflorum were successfully adapted as a whole-cell biocatalyst.
authors.affiliation: College of Pharmacy, Jinan University, 601 Huangpu Avenue West, Guangzhou 510632, China.
authors.forename: Liangbin
authors.initials: L
authors.lastname: Zhou
authors.forename: Ling
authors.initials: L
authors.lastname: Liu
authors.forename: Tian
authors.initials: T
authors.lastname: Tian
authors.forename: Bailin
authors.initials: B
authors.lastname: Xue
authors.forename: Rongmin
authors.initials: R
authors.lastname: Yu
mesh_terms: D000935:Antifungal Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D002384:Catalysis; D003374:Coumarins / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D006027:Glycosides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D018517:Plant Roots / Q000737:chemistry / Q000201:enzymology; D028502:Polygonum / Q000737:chemistry*; D012995:Solubility; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000935:Antifungal Agents; D003374:Coumarins; D006027:Glycosides; D007202:Indicators and Reagents