title: Bone marrow mononuclear cell therapy in experimental allergic asthma: intratracheal versus intravenous administration.
abstract: We hypothesized that the route of administration would impact the beneficial effects of bone marrow-derived mononuclear cell (BMDMC) therapy on the remodelling process of asthma. C57BL/6 mice were randomly assigned to two main groups. In the OVA group, mice were sensitized and challenged with ovalbumin, while the control group received saline using the same protocol. Twenty-four hours before the first challenge, control and OVA animals were further randomized into three subgroups to receive saline (SAL), BMDMCs intravenously (2×10(6)), or BMDMCs intratracheally (2×10(6)). The following changes were induced by BMDMC therapy in OVA mice regardless of administration route: reduction in resistive and viscoelastic pressures, static elastance, eosinophil infiltration, collagen fibre content in airways and lung parenchyma; and reduction in the levels of interleukin (IL)-4, IL-13, transforming growth factor-β and vascular endothelial growth factor. In conclusion, BMDMC modulated inflammatory and remodelling processes regardless of administration route in this experimental model of allergic asthma.
authors.affiliation: Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Soraia C
authors.initials: SC
authors.lastname: Abreu
authors.forename: Mariana A
authors.initials: MA
authors.lastname: Antunes
authors.forename: Tatiana
authors.initials: T
authors.lastname: Maron-Gutierrez
authors.forename: Fernanda F
authors.initials: FF
authors.lastname: Cruz
authors.forename: Debora S
authors.initials: DS
authors.lastname: Ornellas
authors.forename: Adriana L
authors.initials: AL
authors.lastname: Silva
authors.forename: Bruno L
authors.initials: BL
authors.lastname: Diaz
authors.forename: Alexandre M
authors.initials: AM
authors.lastname: Ab'Saber
authors.forename: Vera L
authors.initials: VL
authors.lastname: Capelozzi
authors.forename: Debora G
authors.initials: DG
authors.lastname: Xisto
authors.forename: Marcelo M
authors.initials: MM
authors.lastname: Morales
authors.forename: Patricia R M
authors.initials: PR
authors.lastname: Rocco
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D001249:Asthma / Q000473:pathology* / Q000628:therapy*; D016026:Bone Marrow Transplantation / Q000379:methods*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007963:Leukocytes, Mononuclear / Q000637:transplantation*; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission