title: On-line capillary electrophoresis for enhanced detection sensitivity of feline panleukopenia virus.
abstract: A rapid on-line capillary electrophoresis (CE) method for highly sensitive detection of DNA molecules with specific lengths was developed based on the combination of base stacking (BS) and programmed field strength gradients (PFSG). The BS method has been performed for on-column concentration to improve detection sensitivity without any modification of the CE system. PFSG increased the electrophoretic velocity of DNA molecules, which effectively decreased analysis time. Using the BS and PFSG combination, the amplified PCR product (340-bp DNA) of cats infected with feline panleukopenia virus was detected within 6.5min. Detection sensitivity (∼10-fold) was enhanced compared to conventional CE analysis. The combined on-line CE/BS-PFSG methodology could be an effectively rapid analysis technique for the highly sensitive detection of disease-related specific DNA molecules.
authors.affiliation: Department of Applied Chemistry, College of Applied Science, Kyung Hee University, Yongin-si, Gyunggi-do 446-701, Republic of Korea.
authors.forename: Ahram
authors.initials: A
authors.lastname: Shin
authors.forename: Mijin
authors.initials: M
authors.lastname: Lee
authors.forename: Sangduk
authors.initials: S
authors.lastname: Kim
authors.forename: Seong Ho
authors.initials: SH
authors.lastname: Kang
mesh_terms: D000818:Animals; D002415:Cats; D004279:DNA, Viral / Q000097:blood; D019075:Electrophoresis, Capillary / Q000379:methods*; D005254:Feline Panleukopenia / Q000175:diagnosis / Q000821:virology*; D028301:Feline Panleukopenia Virus / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D004279:DNA, Viral