title: The destructive effects of high-intensity focused ultrasound on hydatid cysts enhanced by ultrasound contrast agent and superabsorbent polymer alone or in combination.
abstract: Cystic echinococcosis, caused by the metacestode stage of Echincoccus granulosus, remains endemic in many regions around the world. The present work evaluated whether or not a superabsorbent polymer (SAP) and ultrasound contrast agent (UCA) alone or in combination could enhance damage efficacy of high-intensity focused ultrasound (HIFU) on hydatid cysts in vitro. HIFU of 100 W acoustic power, with the aid of 0.1 ml UCA and 0.1 g SAP alone or in combination, was used to ablate hydatid cysts in vitro. The comparison of ultrasound image for each layer of hydatid cyst before and after HIFU ablation was made immediately, and the protoscolices of the cysts were stained by eosin exclusion assay, and the structures of protoscolices were observed by light microscopy. To understand the destructive effects of HIFU, the pathological changes in cyst walls of hydatid cyst ablated with HIFU were examined. The results demonstrated that HIFU had some lethal effect on hydatid cysts: echo enhancement of ultrasound image, increase of mortality rate of protoscolices, serious structural damage of protoscolices, and complete destruction or even disappearance of laminated layer and germinal layer was observed in the group of HIFU combined with UCA and SAP alone or in combination. It was found that the destructive effect of HIFU aided with a combination of UCA and SAP to hydatid cysts was more effective than that of HIFU just aided with UCA or SAP alone. These results suggested that UCA and SAP might be used as a HIFU enhancing agent to improve the efficacy of HIFU ablation to hydatid cysts, which could be a possible therapeutic option for cystic echinococcosis.
authors.affiliation: Department of Pathogenic Biology, Chongqing Medical University, 1 Yixueyuan Road, Yuzhong District, Chongqing 400016, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Cai
authors.forename: Lu-Lu
authors.initials: LL
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Ye
authors.forename: Ai-Bo
authors.initials: AB
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Yi-Feng
authors.initials: YF
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D003287:Contrast Media / Q000494:pharmacology*; D004443:Echinococcosis / Q000000981:diagnostic imaging* / Q000473:pathology; D048209:Echinococcus granulosus / Q000033:anatomy & histology / Q000187:drug effects*; D019220:High-Energy Shock Waves; D008853:Microscopy; D013010:Sonication / Q000379:methods*; D016019:Survival Analysis; D014463:Ultrasonography
chemical_list: D000871:Anthelmintics; D003287:Contrast Media