title: The effect of hyperbaric oxygen and air on cartilage tissue engineering.
abstract: There is an urgent need to develop tissue-engineered cartilage for patients experiencing joint malfunction due to insufficient self-repairing capacity of articular cartilage. The aim of this research was to explore the effect of hyperbaric oxygen and air on tissue-engineered cartilage formation from human adipose-derived stem cells seeding on the gelatin/polycaprolactone biocomposites. The results of histological analyses indicate that under hyperbaric oxygen and air stimulation, the cell number of chondrocytes in cartilage matrix was not significantly increased, but the 1,9-dimethylmethylene blue assay showed that the glycosaminoglycans syntheses markedly increased compared to the control group. In quantification real-time polymerase chain reaction results, the chondrogenic-specific gene expression of SOX9, aggrecan, and COL2A1 were compared respectively. Within the limitation of this study, it was concluded that 2.5 atmosphere absolute oxygen and air may provide a stress environment to help cartilage tissue engineering development.
authors.affiliation: Department of Dentistry, National Yang-Ming University, Taipei, Taiwan, Republic of China.
authors.forename: Juin-Hong
authors.initials: JH
authors.lastname: Cherng
authors.forename: Shun-Cheng
authors.initials: SC
authors.lastname: Chang
authors.forename: Shyi-Gen
authors.initials: SG
authors.lastname: Chen
authors.forename: Ming-Lun
authors.initials: ML
authors.lastname: Hsu
authors.forename: Po-Da
authors.initials: PD
authors.lastname: Hong
authors.forename: Shou-Chen
authors.initials: SC
authors.lastname: Teng
authors.forename: Yi-Hsin
authors.initials: YH
authors.lastname: Chan
authors.forename: Chih-Hsin
authors.initials: CH
authors.lastname: Wang
authors.forename: Tim-Mo
authors.initials: TM
authors.lastname: Chen
authors.forename: Niann-Tzyy
authors.initials: NT
authors.lastname: Dai
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000388:Air; D000704:Analysis of Variance; D002356:Cartilage / Q000166:cytology*; D002454:Cell Differentiation; D002470:Cell Survival; D020219:Chondrogenesis / Q000235:genetics; D005109:Extracellular Matrix; D006801:Humans; D006931:Hyperbaric Oxygenation; D013234:Stem Cells / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*