title: Effect of topography of an electrospun nanofiber on modulation of activity of primary rat astrocytes.
abstract: Several biomaterials for neural tissue engineering have recently been proposed for regeneration of damaged tissue and promotion of axonal guidance following CNS injury. When implanted into damaged nerve tissue, biomaterials should favorably induce cell infiltration and axonal guiding while suppressing inflammation. Nanofiber scaffolds are regarded as adequate materials to meet the above requirements; however, most studies of these materials conducted to date have targeted neuronal cells, not glial cells, despite their important function in the injured CNS. In this study, an electrospun nanofibrous scaffold of polycaprolactone (PCL) was investigated with respect to its topographic effects on astrocyte behavior and expression of GFAP. The results revealed that the PCL nanofiber topograghy promoted adhesion, but GFAP expression was down-regulated, leading to reduced astrocytes activity. Taken together, these results indicate that the topographic structure of electrospun nanofibers provides a scaffold that is favorable to neural regeneration via alleviation of astrogliosis.
authors.affiliation: Department of Biological Engineering, Inha University, Incheon, 402-751, Republic of Korea.
authors.forename: Seul Ki
authors.initials: SK
authors.lastname: Min
authors.forename: Sung Hoon
authors.initials: SH
authors.lastname: Kim
authors.forename: Cho Rong
authors.initials: CR
authors.lastname: Kim
authors.forename: Sang-Min
authors.initials: SM
authors.lastname: Paik
authors.forename: Sang-Myung
authors.initials: SM
authors.lastname: Jung
authors.forename: Hwa Sung
authors.initials: HS
authors.lastname: Shin
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D002448:Cell Adhesion; D002470:Cell Survival; D002478:Cells, Cultured; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure*; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005904:Glial Fibrillary Acidic Protein; D011091:Polyesters; C016240:polycaprolactone