title: Bee venom at different concentrations modulates the aeroallergen-induced activation of nasal polyp epithelial cells.
abstract: Bee venom (BV) has long been used as an oriental traditional medicine for the control of pain and inflammation. However, BV's anti-inflammatory mechanisms remain unclear. This study aimed to clarify the potential clinical efficacy of BV concerning the anti-inflammatory effect on nasal epithelial cell inflammation. Nasal polyp epithelial cells were obtained from patients. Cells were exposed to Alternaria alternata, Aspergillus nigra, Dermatophagoides pteronyssinus, Dermatophagoides farina and lipopolysaccharide with or without various concentrations of BV. Interleukin (IL)-6, IL-8, and granulocyte macrophage colony-stimulating factor were measured to determine the activation of epithelial cells. Nuclear factor-ĸB (NF-ĸB) and activator protein 1 expression and activity were determined with Western blot analysis and ELISA. Cytotoxicity of BV was measured using a CellTiter-96® aqueous cell proliferation assay kit. Cell survival was significantly decreased at BV concentrations exceeding 5 µg/ml. Fungi-induced cytokine production was more effectively inhibited by BV than house dust mite. Alternaria enhanced NF-ĸB expression, which was strongly inhibited by BV. BV appears to be relatively safe, and is of potential value for the treatment of airway inflammation and/or immunologic diseases.
authors.affiliation: Department of Otorhinolaryngology, School of Medicine, Catholic University of Daegu, Daegu, South Korea. hsseung@cu.ac.kr
authors.forename: Seung-Heon
authors.initials: SH
authors.lastname: Shin
authors.forename: Mi-Kyung
authors.initials: MK
authors.lastname: Ye
authors.forename: Jung-Kyu
authors.initials: JK
authors.lastname: Kim
authors.forename: Kwan-Kyu
authors.initials: KK
authors.lastname: Park
mesh_terms: D000393:Air Pollutants; D000485:Allergens; D000528:Alternaria; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001234:Aspergillus niger; D001514:Bee Venoms / Q000494:pharmacology*; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D040001:Dermatophagoides farinae; D040002:Dermatophagoides pteronyssinus; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism; D009298:Nasal Polyps; D018808:Transcription Factor AP-1 / Q000378:metabolism
chemical_list: D000393:Air Pollutants; D000485:Allergens; D000893:Anti-Inflammatory Agents; D001514:Bee Venoms; D016207:Cytokines; D016328:NF-kappa B; D018808:Transcription Factor AP-1