title: The effect of unpredictable chronic mild stress on depressive-like behavior and on hippocampal A1 and striatal A2A adenosine receptors.
abstract: This study examined the effects of two chronic stress regimens upon depressive-like behavior, A(1) and A(2A) adenosine receptor binding and immunocontent. Male rats were subjected to unpredictable chronic mild stress (UCMS) or to chronic restraint stress (CRS) for 40 days. Subsequently, depressive-like behaviors (forced swimming and consumption of sucrose) were evaluated, and A(1) adenosine or A(2A) adenosine receptors were examined in the hippocampus or striatum, respectively. UCMS animals demonstrated depressive-related behaviors (decrease in sucrose consumption and increased immobility in the forced swimming test). This group also presented increased A(1) adenosine receptor binding and immunoreactivity in hippocampus, as well as increased striatal A(2A) adenosine receptor binding in the striatum, without alteration in immunoreactivity. Conversely, the chronic restraint stress group displayed only an increase in A(1) adenosine receptor binding and no alteration in the other parameters evaluated. We suggest that the alteration in adenosine receptors, particularly the upregulation of striatal A(2A) adenosine receptors following UCMS, could be associated with depressive-related behavior.
authors.affiliation: Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul, Brazil.
authors.forename: Leonardo M
authors.initials: LM
authors.lastname: Crema
authors.forename: Letícia F
authors.initials: LF
authors.lastname: Pettenuzzo
authors.forename: Michele
authors.initials: M
authors.lastname: Schlabitz
authors.forename: Luisa
authors.initials: L
authors.lastname: Diehl
authors.forename: Juliana
authors.initials: J
authors.lastname: Hoppe
authors.forename: Régis
authors.initials: R
authors.lastname: Mestriner
authors.forename: Daniela
authors.initials: D
authors.lastname: Laureano
authors.forename: Christianne
authors.initials: C
authors.lastname: Salbego
authors.forename: Carla
authors.initials: C
authors.lastname: Dalmaz
authors.forename: Deusa
authors.initials: D
authors.lastname: Vendite
mesh_terms: D000243:Adenosine Deaminase / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D003863:Depression / Q000209:etiology* / Q000473:pathology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D011485:Protein Binding / Q000187:drug effects; D058905:Purinergic Agents / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D043682:Receptor, Adenosine A1 / Q000378:metabolism*; D043705:Receptor, Adenosine A2A / Q000378:metabolism*; D013315:Stress, Psychological / Q000150:complications*; D013395:Sucrose / Q000008:administration & dosage; D013549:Sweetening Agents / Q000008:administration & dosage; D013550:Swimming / Q000523:psychology; D013997:Time Factors; D014316:Tritium / Q000493:pharmacokinetics; D014970:Xanthines / Q000493:pharmacokinetics
chemical_list: D058905:Purinergic Agents; D043682:Receptor, Adenosine A1; D043705:Receptor, Adenosine A2A; D013549:Sweetening Agents; D014970:Xanthines; D014316:Tritium; D013395:Sucrose; C051360:1,3-dipropyl-8-cyclopentylxanthine; D000243:Adenosine Deaminase