title: Selenium pretreatment attenuates formaldehyde-induced genotoxicity in A549 cell lines.
abstract: Formaldehyde is a major industrial chemical and has been extensively used in the manufacture of synthetic resins and chemicals. Numerous studies indicate that formaldehyde can induce various genotoxic effects in vitro and in vivo. A recent study indicated that formaldehyde impaired antioxidant cellular defences and enhanced lipid peroxidation. Selenium is an important antioxidant. We hypothesized that reactive oxygen species (ROS) and lipid peroxidation are involved in formaldehyde-induced genotoxicity in human lung cancer cell line, A549 cell line. To test the hypothesis, we investigated the effects of selenium on formaldehyde-induced genotoxicity in A549 cell lines. The results indicated that exposure to formaldehyde showed the induction of DNA-protein cross-links (DPCs). Formaldehyde significantly increased the malondialdehyde levels and decreased the activities of superoxide dismutase and glutathione peroxidase. In addition, the activations of necrosis factor-κB (NF-κB) and activator protein 1 (AP-1) were induced by the formaldehyde treatment. The pretreatment with selenium counteracted the formaldehyde-induced oxidative stress, ameliorated DPCs and attenuated the activation of NF-κB and AP-1 in A549 cell lines. All the results suggested that the pretreatment with selenium attenuated the formaldehyde-induced genotoxicity through its ROS scavenging and anti-DPCs effects in A549 cell lines.
authors.affiliation: School of Public Health, Medical College, Wuhan University of Science and Technology, Wuhan, Hubei, People's Republic of China.
authors.forename: Yu-Qin
authors.initials: YQ
authors.lastname: Shi
authors.affiliation: School of Public Health, Medical College, Wuhan University of Science and Technology, Wuhan, Hubei, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Wuhan Center for Disease Prevention and Control, Wuhan, Hubei, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Dai
authors.affiliation: Hubei Center for Disease Prevention and Control, Wuhan, Hubei, People's Republic of China.
authors.forename: Zhong-Fa
authors.initials: ZF
authors.lastname: Jiang
authors.affiliation: Hubei Center for Disease Prevention and Control, Wuhan, Hubei, People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: School of Public Health, Medical College, Wuhan University of Science and Technology, Wuhan, Hubei, People's Republic of China zbyan1000@163.com.
authors.forename: Ben-Yan
authors.initials: BY
authors.lastname: Zhang
authors.affiliation: School of Public Health, Medical College, Wuhan University of Science and Technology, Wuhan, Hubei, People's Republic of China.
authors.forename: Zhi-Bing
authors.initials: ZB
authors.lastname: Zhang
mesh_terms: D045744:Cell Line, Tumor; D004249:DNA Damage; D005557:Formaldehyde / Q000633:toxicity*; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D009152:Mutagenicity Tests; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D012643:Selenium / Q000737:chemistry / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D018808:Transcription Factor AP-1 / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D018808:Transcription Factor AP-1; D005557:Formaldehyde; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D012643:Selenium