title: Monoterpenoid indole alkaloids from Alstonia yunnanensis and their cytotoxic and anti-inflammatory activities.
abstract: The 80% ethanol extract of Alstonia yunnanensis afforded five new monoterpenoid indole alkaloids: 11-hydroxy-6,7-epoxy-8-oxo-vincadifformine (1), 14-chloro-15-hydroxy- vincadifformine (2), perakine N(4)-oxide (3), raucaffrinoline N(4)-oxide (4), and vinorine N(1),N(4)-dioxide (5), together with three known compounds: 11-methoxy-6,7-epoxy-8-oxo- vincadifformine (6), vinorine N(4)-oxide (7) and vinorine (8). The structures of the isolated compounds were established based on 1D and 2D (1H-1H-COSY, HMQC, HMBC, and ROESY) NMR spectroscopy, in addition to high resolution mass spectrometry. The isolated compounds were tested in vitro for cytotoxic potential against seven tumor cell lines and anti-inflammatory activities. Compounds 3, 4 and 7 exhibited weak cytotoxicity against the tested cell lines and selective inhibition of Cox-2 (> 85%).
authors.affiliation: Department of Neurosurgery, Institue of Neurology, General Hospital of Shenyang Military Area Command, Shenyang Northern Hospital, #83 Wenhua Road, Shenhe District, Shenyang 110018, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Cao
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
authors.forename: Xu
authors.initials: X
authors.lastname: Gao
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Li
authors.forename: Zhen-Quan
authors.initials: ZQ
authors.lastname: Song
authors.forename: Guobiao
authors.initials: G
authors.lastname: Liang
mesh_terms: D031051:Alstonia / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016861:Cyclooxygenase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D026121:Indole Alkaloids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008968:Molecular Conformation; D039821:Monoterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D016861:Cyclooxygenase Inhibitors; D026121:Indole Alkaloids; D039821:Monoterpenes; D010936:Plant Extracts