title: [Generation of Tau/App/PS1 triple-transgenic mouse model and the study of its biological characteristics].
abstract: OBJECTIVE
  To establish the triple-transgenic mouse model and study their biological characteristics by molecular biology, behavior and pathology.
  
  
  METHODS
  Hybrid the Tau and amyloid precursor protein (APP)/presenilins (PS1) transgenic mouse, the genotype of offspring mice were identified by PCR. Transcribed target genes were detected by RT-PCR. The protein expression of exogenous genes was detected by Western-blot. The pathological change of neurofibrillary tangles and senile plaque were observed by Bielschowsky silver staining and ABC immunohistochemical method. The changes time of learning and memory were observed by Morris water maze.
  
  
  RESULTS
  APP, PS1 and Tau genes were transcript in Tau/APP/PS1 mice. In 6 to 8 months old Tau/APP/PS1 mice, the neurofibrillary tangles and senile plaque could be found in cortex and hippocampus. In 6 months old Tau/APP/PS1 mice, the learning and memory abilities were worse.
  
  
  CONCLUSIONS
  With the behavior change and pathological changes in Tau and beta-amyloid protein (AP), the Tau/APP/PS1 triple-transgenic mice can be used as a further study animal model of AD's pathogenesis and the target of drug treatment.
authors.affiliation: Department of Pharmacology, Zhongshan Medical College, Sun Yat-sen University, Guangzhou, 510080, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Na
authors.forename: Xiao-Nan
authors.initials: XN
authors.lastname: Zhu
authors.forename: Ru-Zhu
authors.initials: RZ
authors.lastname: Chen
authors.forename: Hai
authors.initials: H
authors.lastname: Wang
authors.forename: Xue-Lan
authors.initials: XL
authors.lastname: Wang
mesh_terms: D000544:Alzheimer Disease / Q000473:pathology; D016564:Amyloid beta-Protein Precursor / Q000235:genetics*; D000818:Animals; D001921:Brain / Q000473:pathology; D004195:Disease Models, Animal; D007858:Learning; D008297:Male; D008568:Memory; D051379:Mice; D008822:Mice, Transgenic; D016874:Neurofibrillary Tangles / Q000473:pathology; D058225:Plaque, Amyloid / Q000473:pathology; D053764:Presenilin-1 / Q000235:genetics*; D016875:tau Proteins / Q000235:genetics*
chemical_list: D016564:Amyloid beta-Protein Precursor; D053764:Presenilin-1; D016875:tau Proteins