title: Analysis of the clonality of Candida tropicalis strains from a general hospital in Beijing using multilocus sequence typing.
abstract: Multilocus sequence typing (MLST) based on six loci was used to analyze the relationship of 58 Candida tropicalis isolates from individual patients in a general hospital in Beijing, China. A total of 52 diploid sequence types (DSTs) were generated by the MLST, all of which were new to the central database. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) dendrograms were constructed, which showed that the 58 isolates were distributed robustly and 6 main groups were clustered regardless of the specimen source and medical department. The minimum spanning tree (MST) of the 58 isolates (52 DSTs) and all 401 isolates (268 DSTs) in the C. tropicalis central database (http://pubmlst.org/ctropicalis/) indicated that the isolates in this study clustered in three relative pure clonal complexes, and 2 clustered with isolates from Taiwan, Belgium, Brazil, and the US. This study presents the first MLST analysis of C. tropicalis isolates from Mainland China, which may be useful for further studies on the similarity, genetic relationship, and molecular epidemiology of C. tropicalis strains worldwide.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wu
authors.forename: Haijian
authors.initials: H
authors.lastname: Zhou
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Lianqing
authors.initials: L
authors.lastname: Li
authors.forename: Wenge
authors.initials: W
authors.lastname: Li
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Cui
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
authors.forename: Ruiqi
authors.initials: R
authors.lastname: Cen
authors.forename: Jinxing
authors.initials: J
authors.lastname: Lu
authors.forename: Ying
authors.initials: Y
authors.lastname: Cheng
mesh_terms: D041022:Candida tropicalis / Q000235:genetics* / Q000302:isolation & purification; D002681:China; D030541:Databases, Genetic; D005800:Genes, Fungal; D006769:Hospitals, General; D006801:Humans; D058885:Multilocus Sequence Typing; D010802:Phylogeny