title: Vitrification of early avian blastodermal cells with a new type of cryocontainer.
abstract: Although cryopreservation of avian semen is only applicable for singlegene traits, cryopreservation of avian blastodermal cells could facilitate preservation of the entire genome of endangered or rare-breed poultry. Slow freezing methods result in acceptable survival rates; however, there are apparently no reports regarding the use of vitrification. The aim of the study was to establish methods for chicken embryonic cell vitrification, including development of a container which supported cryopreservation of large numbers of cells (to increase the probability of chimera production). Based on a preliminary study, vitrification seemed to be practical for avian blastodermal cell preservation. Pieces of mosquito net as carrier increased live cell rates compared to pellet form in media containing two macromolecules. Furthermore, we concluded that fetal calf serum in the vitrification medium could be replaced by polyvinylpyrrolidone, a chemically defined substance free of unwanted growth factors and potential pathogens.
authors.affiliation: Institute for Small Animal Research and Co-ordination Centre for Gene Conservation Isaszegi út 200 H-2100 Gödöllő Hungary.
authors.forename: Eszter
authors.initials: E
authors.lastname: Patakiné Várkonyi
authors.forename: Gabriella
authors.initials: G
authors.lastname: Horváth
authors.forename: Nikoletta
authors.initials: N
authors.lastname: Sztán
authors.forename: Eva
authors.initials: E
authors.lastname: Váradi
authors.forename: Judit
authors.initials: J
authors.lastname: Barna
mesh_terms: D000818:Animals; D015925:Cryopreservation; D005615:Freezing; D011200:Poultry; D058989:Vitrification