title: Oligonucleotide-peptide conjugates: solid-phase synthesis under acidic conditions and use in ELISA assays.
abstract: Here we used solid-phase methods to prepare oligonucleotides carrying fibrin/ filaggrin citrullinated peptides. Post-synthetic conjugation protocols were successfully applied for the synthesis of oligonucleotides carrying small peptides. A stepwise protocol using acid treatment for the final deprotection allowed the preparation of polypyrimidine oligonucleotides carrying longer and arginine-rich peptides. An ELISA-based test using  the oligonucleotide-citrullinated peptide conjugates was developed for the detection of  anti-citrullinated protein/peptide antibodies in human serum from rheumatoid  arthritis patients.
authors.affiliation: Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), CIBER-BBN Networking Centre on Bioengineering, Biomaterials and Nanomedicine, Jordi Girona 18, E-08034 Barcelona, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Aviñó
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Gómara
authors.forename: Morteza
authors.initials: M
authors.lastname: Malakoutikhah
authors.forename: Isabel
authors.initials: I
authors.lastname: Haro
authors.forename: Ramon
authors.initials: R
authors.lastname: Eritja
mesh_terms: D000906:Antibodies / Q000097:blood; D001120:Arginine / Q000737:chemistry; D001172:Arthritis, Rheumatoid / Q000175:diagnosis / Q000276:immunology; D002956:Citrulline / Q000138:chemical synthesis / Q000737:chemistry; D000939:Epitopes / Q000097:blood / Q000276:immunology; D005337:Fibrin / Q000138:chemical synthesis / Q000737:chemistry; D000091344:Filaggrin Proteins; D006801:Humans; D007381:Intermediate Filament Proteins / Q000138:chemical synthesis / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D060327:Solid-Phase Synthesis Techniques
chemical_list: D000906:Antibodies; D000939:Epitopes; C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; D009841:Oligonucleotides; D010455:Peptides; D002956:Citrulline; D005337:Fibrin; D001120:Arginine