title: Endogenous signal peptides efficiently mediate the secretion of recombinant proteins in Pichia pastoris.
abstract: By predicting the potential signal peptides from proteins that are naturally secreted by Pichia pastoris, we identified three possible endogenous signal peptides: Scw, Dse and Exg. We compared their capability to mediate the secretion of enhanced green fluorescent protein (EGFP) and Candida antarctica lipase B (CALB) with that of the Saccharomyces cerevisiae α-factor prepro-signal. EGFP entered the secretory pathway of P. pastoris and was efficiently secreted into the culture medium by all three endogenous peptides. Further, these three putative endogenous signal peptides were also effective in secreting CALB. These endogenous signal peptides thus have the potential to mediate the efficient secretion of heterologous proteins in P. pastoris.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, Guangdong, China. liang.shuli@mail.scut.edu.cn
authors.forename: Shuli
authors.initials: S
authors.lastname: Liang
authors.forename: Cheng
authors.initials: C
authors.lastname: Li
authors.forename: Yanrui
authors.initials: Y
authors.lastname: Ye
authors.forename: Ying
authors.initials: Y
authors.lastname: Lin
mesh_terms: D000595:Amino Acid Sequence; D001709:Biotechnology / Q000379:methods; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism*; D018628:Gene Dosage; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008049:Lipase / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010843:Pichia / Q000235:genetics* / Q000378:metabolism*; D021382:Protein Sorting Signals / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D008049:Lipase; C431349:lipase B, Candida antarctica