title: Quantitative proteomics by amino acid labeling in foot-and-mouth disease virus (FMDV)-infected cells.
abstract: Foot-and-mouth disease virus (FMDV) is an important disease agent that can be difficult to effectively eradicate from herds. Because it is an obligate intracellular parasite, the virus has multiple effects on the host cell during infection. Here, a high-throughput quantitative proteomic approach was used to develop an unbiased holistic overview of the protein changes in IBRS-2 cells infected with FMDV. Stable isotope labeling with amino acids in cell culture (SILAC) combined with LC-MS/MS was performed to identify and quantify 1260 cellular and 2 viral proteins after 6 h of infection of IBRS-2 cells with FMDV. Of these identified and measured cellular protein pairs, 77 were significantly up-regulated, and 50 were significantly down-regulated based on significance B ≤ 0.05. The differentially altered proteins included a number of proteins involved in endolysosomal proteases system, cell cycle, cellular growth and proliferation, and immune cell trafficking. Selected data were validated by Western blot. Ingenuity Pathway Analysis revealed that proteins that changed in response to infection could be assigned to defined canonical pathways and functional groupings, such as integrin signaling. The obtained data might not only improve the understanding of the dynamics of FMDV and host interaction but may also help elucidate the pathogenic mechanism of FMDV infection.
authors.affiliation: Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou 510642, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Ye
authors.forename: Guangrong
authors.initials: G
authors.lastname: Yan
authors.forename: Yongwen
authors.initials: Y
authors.lastname: Luo
authors.forename: Tiezhu
authors.initials: T
authors.lastname: Tong
authors.forename: Xiangtao
authors.initials: X
authors.lastname: Liu
authors.forename: Chaoan
authors.initials: C
authors.lastname: Xin
authors.forename: Ming
authors.initials: M
authors.lastname: Liao
authors.forename: Huiying
authors.initials: H
authors.lastname: Fan
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002460:Cell Line / Q000821:virology; D002853:Chromatography, Liquid; D015536:Down-Regulation; D005069:Evaluation Studies as Topic; D005536:Foot-and-Mouth Disease / Q000235:genetics / Q000378:metabolism / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000302:isolation & purification / Q000472:pathogenicity; D054884:Host-Pathogen Interactions / Q000235:genetics*; D007553:Isotope Labeling; D040901:Proteomics; D013552:Swine / Q000821:virology; D053719:Tandem Mass Spectrometry; D015854:Up-Regulation
chemical_list: D000596:Amino Acids