title: Phosphorylation of VE-cadherin is modulated by haemodynamic forces and contributes to the regulation of vascular permeability in vivo.
abstract: Endothelial adherens junctions maintain vascular integrity. Arteries and veins differ in their permeability but whether organization and strength of their adherens junctions vary has not been demonstrated in vivo. Here we report that vascular endothelial cadherin, an endothelial specific adhesion protein located at adherens junctions, is phosphorylated in Y658 and Y685 in vivo in veins but not in arteries under resting conditions. This difference is due to shear stress-induced junctional Src activation in veins. Phosphorylated vascular endothelial-cadherin is internalized and ubiquitinated in response to permeability-increasing agents such as bradykinin and histamine. Inhibition of Src blocks vascular endothelial cadherin phosphorylation and bradykinin-induced permeability. Point mutation of Y658F and Y685F prevents vascular endothelial cadherin internalization, ubiquitination and an increase in permeability by bradykinin in vitro. Thus, phosphorylation of vascular endothelial cadherin contributes to a dynamic state of adherens junctions, but is not sufficient to increase vascular permeability in the absence of inflammatory agents.
authors.affiliation: FIRC Institute of Molecular Oncology, Via Adamello 16, 20139 Milan, Italy.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Orsenigo
authors.forename: Costanza
authors.initials: C
authors.lastname: Giampietro
authors.forename: Aldo
authors.initials: A
authors.lastname: Ferrari
authors.forename: Monica
authors.initials: M
authors.lastname: Corada
authors.forename: Ariane
authors.initials: A
authors.lastname: Galaup
authors.forename: Sara
authors.initials: S
authors.lastname: Sigismund
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Ristagno
authors.forename: Luigi
authors.initials: L
authors.lastname: Maddaluno
authors.forename: Gou Young
authors.initials: GY
authors.lastname: Koh
authors.forename: Davide
authors.initials: D
authors.lastname: Franco
authors.forename: Vartan
authors.initials: V
authors.lastname: Kurtcuoglu
authors.forename: Dimos
authors.initials: D
authors.lastname: Poulikakos
authors.forename: Peter
authors.initials: P
authors.lastname: Baluk
authors.forename: Donald
authors.initials: D
authors.lastname: McDonald
authors.forename: Maria
authors.initials: M
authors.lastname: Grazia Lampugnani
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Dejana
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity / Q000187:drug effects; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism*; D001158:Arteries / Q000187:drug effects / Q000502:physiology; D001920:Bradykinin / Q000494:pharmacology; D015820:Cadherins / Q000276:immunology / Q000378:metabolism*; D002199:Capillary Permeability / Q000187:drug effects; D004705:Endocytosis / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D006439:Hemodynamics / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010766:Phosphorylation / Q000187:drug effects; D013314:Stress, Mechanical; D054875:Ubiquitination / Q000187:drug effects; D014680:Veins / Q000187:drug effects / Q000502:physiology; D019061:src-Family Kinases / Q000378:metabolism; D000099152:Cadherin 5
chemical_list: D015703:Antigens, CD; D001920:Bradykinin; D015820:Cadherins; D018836:Inflammation Mediators; D019061:src-Family Kinases; D000099152:Cadherin 5