title: Evaluation of the VITEK 2 gram positive (GP) microbial identification test card: collaborative study.
abstract: A collaborative study was conducted to evaluate the performance of the VITEK 2 Gram Positive (GP) identification card for use with the VITEK 2 automated microbial identification system. The GP test card is used in the identification of selected Gram positive organisms, including Listeria and Staphylococcus species. The VITEK 2 GP card is based on 43 biochemical tests measuring carbon source utilization, inhibition and resistance, and enzymatic activities. A total of 20 laboratories representing government, industry, and private testing laboratories throughout the United States participated. In this study, 720 Gram-positive inclusivity isolates were analyzed by the GP Identification method. Of the 720 well-characterized isolates, 714 were identified correctly, zero were misidentified, zero were unidentified, and six were not characterized as a Gram-positive organism by the VITEK 2 GP method. Additionally, 120 strains exclusive of Gram-positive organisms were screened by Gram stain. A total of 106 isolates were correctly excluded. Fourteen organisms were incorrectly characterized by Gram stain procedures, thus resulting in improper analysis and misidentification by VITEK GP. The VITEK 2 GP identification method is an acceptable automated method for the rapid identification of selected Gram-positive bacteria.
authors.affiliation: Q Laboratories, Inc., 1400 Harrison Ave, Cincinnati, OH 45214, USA.
authors.forename: Erin
authors.initials: E
authors.lastname: Crowley
authors.forename: Patrick
authors.initials: P
authors.lastname: Bird
authors.forename: Kiel
authors.initials: K
authors.lastname: Fisher
authors.forename: Katherine
authors.initials: K
authors.lastname: Goetz
authors.forename: Megan
authors.initials: M
authors.lastname: Boyle
authors.forename: M Joseph
authors.initials: MJ
authors.lastname: Benzinger
authors.forename: Marc
authors.initials: M
authors.lastname: Juenger
authors.forename: James
authors.initials: J
authors.lastname: Agin
authors.forename: David
authors.initials: D
authors.lastname: Goins
authors.forename: Ronald L
authors.initials: RL
authors.lastname: Johnson
mesh_terms: D015373:Bacterial Typing Techniques / Q000295:instrumentation* / Q000379:methods*; D006094:Gram-Positive Bacteria / Q000302:isolation & purification*; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D007753:Laboratories; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D011933:Reagent Kits, Diagnostic