title: Genome-wide characterization of the CBF/DREB1 gene family in Brassica rapa.
abstract: The C-repeat/dehydration-responsive element binding transcription factors (CBF/DREBs) are important proteins in involved in responses to abiotic stress in plants. We identified ten BrDREB1 genes belonging to the CBF/DREB1 gene family in the Brassica rapa whole genome sequence, whereas six genes are found in the Arabidopsis thaliana genome. The deduced amino acid sequences of the B. rapa genes showed conserved motifs shared with other known plant CBF/DREB1s. Comparative analysis revealed that nine of the BrDREB1 genes were derived from the recent genome triplication in the tribe Brassiceae and the other one was translocated. The nine genes were located in seven of the 12 macrosyntenic blocks that are triplicated counterparts of four Arabidopsis macrosyntenic blocks harboring six CBF/DREB1 genes: one gene on each of three blocks and three tandemly arrayed genes on another block. We inspected the expression patterns of eight BrDREB1 genes by RT-PCR and microarray database searches. All eight genes were highly up-regulated during cold (4 °C) treatment, and some of them were also responsive to salt (250 mM NaCl), drought (air drying), and ABA (100 μM) treatment. Microarray data for plant developmental stages revealed that BrDREB1C2 was highly expressed during a period of cold treatment for vernalization, similar to abiotic stress-inducible genes homologous to Bn28a, Bn47, Bn115, and BoRS1, but almost opposite of BrFLC genes. Taken together, the number of BrDREB1 genes increased to 10 by genome triplication and reorganization, providing additional functions in B. rapa abiotic stress responses and development, as distinct from their Arabidopsis homologs.
authors.affiliation: Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Science, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Sang-Choon
authors.initials: SC
authors.lastname: Lee
authors.forename: Myung-Ho
authors.initials: MH
authors.lastname: Lim
authors.forename: Jae-Gyeong
authors.initials: JG
authors.lastname: Yu
authors.forename: Beom-Seok
authors.initials: BS
authors.lastname: Park
authors.forename: Tae-Jin
authors.initials: TJ
authors.lastname: Yang
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000222:Adaptation, Physiological / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics; D001937:Brassica / Q000235:genetics*; D003080:Cold Temperature; D004261:DNA Replication; D055864:Droughts; D019143:Evolution, Molecular; D015870:Gene Expression; D017343:Genes, Plant; D018745:Genome, Plant; D006360:Heat-Shock Proteins / Q000235:genetics; D046228:Microarray Analysis; D063245:Plant Development / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D055049:Salt Tolerance / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride; D013312:Stress, Physiological / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014178:Translocation, Genetic; D015854:Up-Regulation
chemical_list: D029681:Arabidopsis Proteins; C104898:BN28 protein, Brassica napus; C114578:DREB1A protein, Arabidopsis; D006360:Heat-Shock Proteins; D010940:Plant Proteins; D014157:Transcription Factors; D012965:Sodium Chloride; D000040:Abscisic Acid