title: Mitochondrial respiration in human viable platelets--methodology and influence of gender, age and storage.
abstract: Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ex-vivo analysis with the need for minute sample amounts and excellent reproducibility and stability.
authors.affiliation: Mitochondrial Pathophysiology Unit, Lund University, 221 84 Lund, Sweden. fredrik.sjovall@med.lu.se
authors.forename: Fredrik
authors.initials: F
authors.lastname: Sjövall
authors.forename: Johannes K H
authors.initials: JK
authors.lastname: Ehinger
authors.forename: Sigurður E
authors.initials: SE
authors.lastname: Marelsson
authors.forename: Saori
authors.initials: S
authors.lastname: Morota
authors.forename: Eleonor Asander
authors.initials: EA
authors.lastname: Frostner
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Uchino
authors.forename: Johan
authors.initials: J
authors.lastname: Lundgren
authors.forename: Einar
authors.initials: E
authors.lastname: Arnbjörnsson
authors.forename: Magnus J
authors.initials: MJ
authors.lastname: Hansson
authors.forename: Vineta
authors.initials: V
authors.lastname: Fellman
authors.forename: Eskil
authors.initials: E
authors.lastname: Elmér
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D001792:Blood Platelets / Q000378:metabolism*; D019069:Cell Respiration; D002648:Child; D002675:Child, Preschool; D003584:Cytological Techniques / Q000379:methods; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism*; D012737:Sex Factors; D013048:Specimen Handling / Q000379:methods; D055815:Young Adult