title: Gs-coupled adenosine receptors differentially limit antigen-induced mast cell activation.
abstract: Mast cell activation results in the immediate release of proinflammatory mediators prestored in cytoplasmic granules, as well as initiation of lipid mediator production and cytokine synthesis by these resident tissue leukocytes. Allergen-induced mast cell activation is central to the pathogenesis of asthma and other allergic diseases. Presently, most pharmacological agents for the treatment of allergic disease target receptors for inflammatory mediators. Many of these mediators, such as histamine, are released by mast cells. Targeting pathways that limit antigen-induced mast cell activation may have greater therapeutic efficacy by inhibiting the synthesis and release of many proinflammatory mediators produced in the mast cell. In vitro studies using cultured human and mouse mast cells, and studies of mice lacking A(2B) receptors, suggest that adenosine receptors, specifically the G(s)-coupled A(2A) and A(2B) receptors, might provide such a target. Here, using a panel of mice lacking various combinations of adenosine receptors, and mast cells derived from these animals, we show that adenosine receptor agonists provide an effective means of inhibition of mast cell degranulation and induction of cytokine production both in vitro and in vivo. We identify A(2B) as the primary receptor limiting mast cell degranulation, whereas the combined activity of A(2A) and A(2B) is required for the inhibition of cytokine synthesis.
authors.affiliation: Department of Medicine, Division of Pulmonary and Critical Care Medicine, University of North Carolina at Chapel Hill, 8033 Burnett-Womack, Chapel Hill, NC 27599-7219, USA.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Hua
authors.forename: Kelly D
authors.initials: KD
authors.lastname: Chason
authors.forename: Corey
authors.initials: C
authors.lastname: Jania
authors.forename: Tatiana
authors.initials: T
authors.lastname: Acosta
authors.forename: Catherine
authors.initials: C
authors.lastname: Ledent
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Tilley
mesh_terms: D058908:Adenosine A2 Receptor Agonists / Q000494:pharmacology*; D000818:Animals; D000941:Antigens / Q000494:pharmacology; D015550:Cell Degranulation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D004140:Dinitrophenols / Q000494:pharmacology; D005260:Female; D019205:GTP-Binding Protein alpha Subunits, Gs / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010323:Passive Cutaneous Anaphylaxis / Q000276:immunology; D043705:Receptor, Adenosine A2A / Q000235:genetics / Q000378:metabolism*; D043704:Receptor, Adenosine A2B / Q000235:genetics / Q000378:metabolism*; D012709:Serum Albumin / Q000494:pharmacology
chemical_list: D058908:Adenosine A2 Receptor Agonists; D000941:Antigens; D016207:Cytokines; D004140:Dinitrophenols; D043705:Receptor, Adenosine A2A; D043704:Receptor, Adenosine A2B; D012709:Serum Albumin; C058064:dinitrophenyl-human serum albumin conjugate; D019205:GTP-Binding Protein alpha Subunits, Gs