title: Problem-solving test: nucleocytoplasmic shuttling of pre-mRNA binding proteins.
abstract: Terms to be familiar with before you start to solve the test: transcription, pre-mRNA, RNA processing, RNA transport, RNA polymerase II, direct and indirect immunofluorescence staining, cell fractionation by centrifugation, oligo(dT)-cellulose chromatography, washing and elution of the column, ribonuclease, SDS-polyacrylamide gel electrophoresis, Western-blotting, pre-ribosomal particles, nuclear envelope, heterokaryon, phase contrast microscopy, polyadenylated RNA.
authors.affiliation: Department of Medical Biology, University of Pécs Medical School, H-7624 Pécs, Hungary. jozsef.szeberenyi@aok.pte.hu
authors.forename: József
authors.initials: J
authors.lastname: Szeberényi
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000502:physiology; D000818:Animals; D001671:Biochemistry / Q000193:education / Q000379:methods; D006801:Humans; D011340:Problem Solving; D012319:RNA Polymerase II / Q000737:chemistry* / Q000378:metabolism; D012322:RNA Precursors / Q000737:chemistry* / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional / Q000502:physiology; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D013663:Teaching / Q000379:methods*
chemical_list: D012322:RNA Precursors; D016601:RNA-Binding Proteins; D012319:RNA Polymerase II