title: First live birth in germany after re-transplantation of cryopreserved ovarian tissue: original device for initiation of ice formation.
abstract: BACKGROUND
  The problem of post-cancer infertility is of significant concern. The cryopreservation of ovarian tissue before cancer therapy with retransplantation after convalescence is the key to solving this problem.
  
  
  METHODS
  Cryopreservation of ovarian tissue was performed in 2005 after surgical operation, post-operative low-temperature 22 hour transportation, and freezing using a special, original design block constructed for the initiation of ice formation (ice-seeding). We present the construction and function of this block.
  
  
  RESULTS
  In 2011, it was noted that a baby was born after thawing and re-transplantation of ovarian tissue. The technical and biological aspects of initiated crystals formation in the process of cryopreservation are emphasised and discussed.
  
  
  CONCLUSIONS
  The first live birth in Germany after re-transplantation of cryopreserved ovarian tissue was noted. This cryopreservation was performed using the protocol described here. Block for auto-seeding of principally new construction recommended.
authors.affiliation: Universitotsfrauenklinik Cologne, Cologne, Germany.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Isachenko
authors.forename: Evgenia
authors.initials: E
authors.lastname: Isachenko
authors.forename: Gudrun
authors.initials: G
authors.lastname: Keck
authors.forename: Ralf
authors.initials: R
authors.lastname: Dittrich
authors.forename: Markus
authors.initials: M
authors.lastname: Montag
authors.forename: Hans
authors.initials: H
authors.lastname: van der Ven
authors.forename: Peter
authors.initials: P
authors.lastname: Mallmann
authors.forename: Andreas
authors.initials: A
authors.lastname: Müller
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Distler
authors.forename: Matthias W
authors.initials: MW
authors.lastname: Beckmann
authors.forename: Gohar
authors.initials: G
authors.lastname: Rahimi
mesh_terms: D000328:Adult; D015925:Cryopreservation / Q000379:methods*; D003460:Crystallization; D005260:Female; D059247:Fertility Preservation / Q000379:methods*; D005615:Freezing; D005858:Germany; D006801:Humans; D007053:Ice; D007247:Infertility, Female / Q000139:chemically induced / Q000628:therapy*; D050498:Live Birth; D010053:Ovary / Q000502:physiology / Q000637:transplantation*; D011247:Pregnancy; D014182:Transplantation, Autologous
chemical_list: D007053:Ice