title: The mammalian proteins MMS19, MIP18, and ANT2 are involved in cytoplasmic iron-sulfur cluster protein assembly.
abstract: Iron-sulfur (Fe-S) clusters are essential cofactors of proteins with a wide range of biological functions. A dedicated cytosolic Fe-S cluster assembly (CIA) system is required to assemble Fe-S clusters into cytosolic and nuclear proteins. Here, we show that the mammalian nucleotide excision repair protein homolog MMS19 can simultaneously bind probable cytosolic iron-sulfur protein assembly protein CIAO1 and Fe-S proteins, confirming that MMS19 is a central protein of the CIA machinery that brings Fe-S cluster donor proteins and the receiving apoproteins into proximity. In addition, we show that mitotic spindle-associated MMXD complex subunit MIP18 also interacts with both CIAO1 and Fe-S proteins. Specifically, it binds the Fe-S cluster coordinating regions in Fe-S proteins. Furthermore, we show that ADP/ATP translocase 2 (ANT2) interacts with Fe-S apoproteins and MMS19 in the CIA complex but not with the individual proteins. Together, these results elucidate the composition and interactions within the late CIA complex.
authors.affiliation: European Research Institute for the Biology of Ageing, University Medical Center Groningen, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
authors.forename: Niek
authors.initials: N
authors.lastname: van Wietmarschen
authors.forename: Annie
authors.initials: A
authors.lastname: Moradian
authors.forename: Gregg B
authors.initials: GB
authors.lastname: Morin
authors.forename: Peter M
authors.initials: PM
authors.lastname: Lansdorp
authors.forename: Evert-Jan
authors.initials: EJ
authors.lastname: Uringa
mesh_terms: D033742:Adenine Nucleotide Translocator 2 / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D056511:Metallochaperones / Q000235:genetics / Q000378:metabolism; D008667:Metalloproteins; D051379:Mice; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D033742:Adenine Nucleotide Translocator 2; C112111:CIAO1 protein, human; C569498:CIAO2B protein, human; D002352:Carrier Proteins; C416322:MMS19 protein, human; C467337:MMS19 protein, mouse; D056511:Metallochaperones; D008667:Metalloproteins; D046912:Multiprotein Complexes; D009687:Nuclear Proteins; D014157:Transcription Factors