title: Novosphingobium sediminis sp. nov., isolated from the sediment of a eutrophic lake.
abstract: A Gram-negative, rod-shaped, aerobic, non-motile, bacterium (YG-17(T)) was isolated from eutrophic Taihu lake sediment. Colonies grown on YG agar plates were circular, convex, and yellow-colored. On the basis of 16S rRNA gene sequence similarity, strain YG-17(T) was shown to be closely related to Novosphingobium aromaticivorum (97.9%), followed by Novosphingobium stygium (97.5%), Novosphingobium subterranea (96.9%) and Novosphingobium taihuense (96.7%). The DNA-DNA relatedness values of strain YG-17(T) to the most phylogenetically related species N. aromaticivorum and N. stygium were 14% and 21%, respectively. The G+C content of the genomic DNA was 63.2 mol%. The major respiratory quinone was ubiquinone-10 and major fatty acids were C(18:1 ω7c), C(17:1 ω6c) and C(14:0)2-OH. Sphingoglycolipids were present, and spermidine was detected as the major polyamine. According to comparative physiological and chemotaxonomic analysis, strain YG-17(T) is proposed to be a novel species of the genus Novosphingobium, with the name of Novosphingobium sediminis sp. nov. The type strain is YG-17(T) (NBRC 106119(T)= CGMCC 1.9114(T)).
authors.affiliation: College of Biological Sciences, Key Laboratory of Agro-Microbial Resource and Utilization, Ministry of Agriculture, China Agricultural University, Beijing, People’s Republic of China.
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Li
authors.forename: Zhuo-Tao
authors.initials: ZT
authors.lastname: Zou
authors.forename: Bao-Zhen
authors.initials: BZ
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Jin-Shui
authors.initials: JS
authors.lastname: Yang
authors.forename: Hong-Li
authors.initials: HL
authors.lastname: Yuan
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005798:Genes, Bacterial; D019015:Geologic Sediments / Q000382:microbiology*; D060106:Lakes / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013095:Spermidine / Q000032:analysis; D013107:Sphingolipids / Q000032:analysis; D014451:Ubiquinone / Q000032:analysis; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D013107:Sphingolipids; D014451:Ubiquinone; C025204:Ubiquinone Q2; D013095:Spermidine