title: Rapid capillary electrochromatographic profiling of phytohormones on a hydrophilic interaction/strong anion-exchange mixed-mode monolith.
abstract: An improved hydrophilic interaction/strong anion-exchange (HI-SAX) monolith for rapid capillary electrochromatographic profiling has been developed to detect carboxylic phytohormones with high resolution. The HI-SAX monolith was prepared by copolymerization of 2-(methacryloyloxy)ethyltrimethylammonium methyl sulfate, pentaerythritol triacrylate and porogenic solvents. Detailed polymerization compositions were investigated and improved, and the characteristics in terms of morphology, retention mechanism and column reproducibility were studied. A typical hydrophilic chromatography mechanism was observed when ACN content exceeded 30%, and the HI-SAX mixed-mode was verified with nucleic acid bases and nucleosides. Based on the improved HI-SAX monolith, capillary electrochromatographic profiling of typical phytohormones was evaluated by using indole-3-acetic acid (IAA), abscisic acid (ABA) and gibberellic acid (GA(3)) as the mode analytes. Effects of the test parameters on carboxyl phytohormones were investigated, and a fast analysis with high resolution for the representative phytohormones was achieved within 4.0 min with intraday RSDs (n = 3) less than 2.6% and 6.3% for the retention time and peak area, respectively. When applied to spiked corn samples, mean recoveries between 82.3% and 95.4% were obtained.
authors.affiliation: Institute of Food Safety and Environmental Monitoring, Fuzhou University, 350108, China. xulin@fzu.edu.cn
authors.forename: Xucong
authors.initials: X
authors.lastname: Lin
authors.forename: Yanping
authors.initials: Y
authors.lastname: Li
authors.forename: Dongjie
authors.initials: D
authors.lastname: Xu
authors.forename: Chunmei
authors.initials: C
authors.lastname: Yang
authors.forename: Zenghong
authors.initials: Z
authors.lastname: Xie
mesh_terms: D000040:Abscisic Acid / Q000737:chemistry; D000837:Anion Exchange Resins / Q000138:chemical synthesis; D053801:Capillary Electrochromatography / Q000379:methods*; D005875:Gibberellins / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D007210:Indoleacetic Acids / Q000737:chemistry; D010937:Plant Growth Regulators / Q000032:analysis* / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D000837:Anion Exchange Resins; D005875:Gibberellins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; C030737:indoleacetic acid; D000040:Abscisic Acid; C007842:gibberellic acid