title: Factor VIIa regulates the expression of caspase-3, MMP-9, and CD44 in SW620 colon cancer cells involving PAR2/MAPKs/NF-κB signaling pathways.
abstract: Our previous study has demonstrated that TF/FVIIa and PAR2 are closely related to the proliferation and migration of colon cancer cell line SW620. However, the detailed molecular mechanisms in the process remain unclear. This study further investigated whether some important molecules (caspase-3, MMP-9 and CD44) are involved in the events. The results showed that PAR2-AP or FVIIa elicited time-dependent downregulation of caspase-3, and up-regulation of MMP-9 and CD44 in SW620 cells. The effects of FVIIa were TF-dependent and involving PAR2/MAPKs/NF-κB signal transduction pathways. Our study suggests that the links among PAR2/MAPKs/NF-κB may be blocked for effective treatments of colorectal cancers.
authors.affiliation: Department of Internal Medicine, Affiliated Hospital of Jiangsu University, Jiangsu, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wu
authors.forename: Xianmei
authors.initials: X
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.forename: Dongdong
authors.initials: D
authors.lastname: Chen
authors.forename: Hongxiang
authors.initials: H
authors.lastname: Xie
authors.forename: Yuan
authors.initials: Y
authors.lastname: Mu
authors.forename: Biao
authors.initials: B
authors.lastname: Wu
authors.forename: Jinchuan
authors.initials: J
authors.lastname: Yan
mesh_terms: D053148:Caspase 3 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D003110:Colonic Neoplasms / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015536:Down-Regulation; D015942:Factor VIIa / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D020935:MAP Kinase Signaling System; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D044464:Receptor, PAR-2 / Q000235:genetics* / Q000378:metabolism*; D015398:Signal Transduction; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*
chemical_list: C497483:CD44 protein, human; D018960:Hyaluronan Receptors; D016328:NF-kappa B; D044464:Receptor, PAR-2; D048051:p38 Mitogen-Activated Protein Kinases; D015942:Factor VIIa; D053148:Caspase 3; D020780:Matrix Metalloproteinase 9