title: Highly regioselective glycosylation of xylosyl-containing taxanes by Enterobacter cloaceae.
abstract: Three new diglycoside taxanes (4-6) containing an additional α-d-glucose unit were obtained via Enterobacter cloaceae-mediated, highly regioselective glycosylations at OH-4 of the xylosyl moieties in 7-xylosyl taxanes (1-3). Their structures were determined on the basis of extensive spectroscopic analysis and chemical reactivity. This is the first report of an enzymatic elongation of the saccharide chain in taxanes, and the glycosylation reaction is the first described example of a transformation effected by E. cloaceae.
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, 1 Xian Nong Tan Street, Beijing 100050, PR China.
authors.forename: Kang
authors.initials: K
authors.lastname: Wang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zou
authors.forename: Ridao
authors.initials: R
authors.lastname: Chen
authors.forename: Dan
authors.initials: D
authors.lastname: Xie
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Jungui
authors.initials: J
authors.lastname: Dai
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D016972:Enterobacter cloacae / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D015394:Molecular Structure; D043823:Taxoids / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D043823:Taxoids