title: Cloning and characterization of a fish specific gelsolin family gene, ScinL, in olive flounder (Paralichthys olivaceus).
abstract: Scinderin like (ScinL) gene is a unique gelsolin family gene found only in fish. In this study ScinL gene was cloned in olive flounder for the first time and characterized its expression and function. Flounder ScinL cDNA consists of 2911 nucleotides encoding a putative protein of 720 amino acids (79.4 kDa). In phylogenetic analysis, flounder ScinL is closely related to ScinL of zebra fish, anableps, and fugu with the similarity of 51-72%. Fish ScinLs are positioned between gelsolin and scinderin of other species. Flounder ScinL protein has the highly conserved actin and PIP2 binding sites, Ca(2+) coordination site, and a C-terminal latch helix preventing the activation of ScinL protein in the absence of Ca(2+). Putative binding sites for NFAT and AP-1 were found in 5' flanking region. Constitutive ScinL expression was found in most organs and the expression level was higher in gill, head kidney, trunk kidney, spleen and skin than muscle, stomach, intestine and brain. In Q-PCR analysis ScinL and CYP1A1 gene expression were significantly upregulated by BaP in head kidney in vivo and in vitro, and in macrophage cells. Upregulated ScinL expression by BaP was blocked by EGTA, indicating a calcium dependent regulation of ScinL expression.
authors.affiliation: Department of Marine Biotechnology, Gangneung Wonju National University, Gangneung 210-702, South Korea.
authors.forename: Deokhwe
authors.initials: D
authors.lastname: Hur
authors.forename: Suhee
authors.initials: S
authors.lastname: Hong
mesh_terms: D024506:5' Flanking Region / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001564:Benzo(a)pyrene / Q000494:pharmacology; D001665:Binding Sites / Q000235:genetics; D002118:Calcium / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004533:Egtazic Acid / Q000494:pharmacology; D029941:Fish Proteins / Q000235:genetics*; D005432:Flounder / Q000235:genetics*; D018260:Gelsolin / Q000145:classification / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D005880:Gills / Q000378:metabolism; D060226:Head Kidney / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000378:metabolism
chemical_list: D002614:Chelating Agents; D018076:DNA, Complementary; D029941:Fish Proteins; D018260:Gelsolin; D001564:Benzo(a)pyrene; D004533:Egtazic Acid; D002118:Calcium