title: Synthesis, biological evaluation, and live cell imaging of novel fluorescent duocarmycin analogs.
abstract: For a better understanding of the mode of action of duocarmycin and its analogs, the novel fluorescent duocarmycin derivatives 13-15 and 17b-19b were synthesized, and their bioactivity as well as their cellular uptake investigated using confocal laser scanning microscopy (CLSM) in live-cell imaging experiments.
authors.affiliation: Georg-August-Universität Göttingen, Institut für Organische und Biomolekulare Chemie, Tammannstrasse 2, D-37077 Göttingen, Germany. ltietze@gwdg.de
authors.forename: Lutz F
authors.initials: LF
authors.lastname: Tietze
authors.forename: Frank
authors.initials: F
authors.lastname: Behrendt
authors.forename: Galina F
authors.initials: GF
authors.lastname: Pestel
authors.forename: Ingrid
authors.initials: I
authors.lastname: Schuberth
authors.forename: Mišo
authors.initials: M
authors.lastname: Mitkovski
mesh_terms: D018906:Antineoplastic Agents, Alkylating / Q000032:analysis* / Q000138:chemical synthesis / Q000493:pharmacokinetics / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D060326:Chemistry Techniques, Synthetic; D000080890:Duocarmycins; D005456:Fluorescent Dyes / Q000032:analysis* / Q000138:chemical synthesis / Q000493:pharmacokinetics / Q000494:pharmacology; D006801:Humans; D007211:Indoles / Q000032:analysis* / Q000138:chemical synthesis / Q000493:pharmacokinetics / Q000494:pharmacology; D018613:Microscopy, Confocal / Q000379:methods*; D009369:Neoplasms / Q000188:drug therapy; D011760:Pyrrolidinones / Q000032:analysis / Q000138:chemical synthesis / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D018906:Antineoplastic Agents, Alkylating; D000080890:Duocarmycins; D005456:Fluorescent Dyes; D007211:Indoles; D011760:Pyrrolidinones; C058289:duocarmycin A