title: Imaging mass spectrometry and genome mining reveal highly antifungal virulence factor of mushroom soft rot pathogen.
abstract: Caught in the act: imaging mass spectrometry of a button mushroom infected with the soft rot pathogen Janthinobacterium agaricidamnosum in conjunction with genome mining revealed jagaricin as a highly antifungal virulence factor that is not produced under standard cultivation conditions. The structure of jagaricin was rigorously elucidated by a combination of physicochemical analyses, chemical derivatization, and bioinformatics.
authors.affiliation: Department of Biomolecular Chemistry, Leibniz Institute for Natural Product Research and Infection Biology, HKI, Jena, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Graupner
authors.forename: Kirstin
authors.initials: K
authors.lastname: Scherlach
authors.forename: Tom
authors.initials: T
authors.lastname: Bretschneider
authors.forename: Gerald
authors.initials: G
authors.lastname: Lackner
authors.forename: Martin
authors.initials: M
authors.lastname: Roth
authors.forename: Harald
authors.initials: H
authors.lastname: Gross
authors.forename: Christian
authors.initials: C
authors.lastname: Hertweck
mesh_terms: D000363:Agaricales / Q000254:growth & development; D000935:Antifungal Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D019295:Computational Biology; D005658:Fungi / Q000187:drug effects; D016680:Genome, Bacterial; D006090:Gram-Negative Bacteria / Q000235:genetics* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D010453:Peptide Synthases / Q000235:genetics / Q000378:metabolism; D010456:Peptides, Cyclic / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000382:microbiology; D048630:Polyketide Synthases / Q000235:genetics / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000935:Antifungal Agents; D010456:Peptides, Cyclic; C581530:jagaricin; D048630:Polyketide Synthases; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase