title: Vogesella alkaliphila sp. nov., isolated from an alkaline soil, and emended description of the genus Vogesella.
abstract: Strain JC141(T) was isolated from an alkaline soil (pH 8.8) at Mau, Uttar Pradesh, India. Colonies were blue with a metallic sheen; cells stained Gram-negative, and were oxidase- and catalase-positive, but chitinase-negative. Major fatty acids were C16:1ω7c/C16:1ω6c, C16:0 and C18:0 with minor amounts of C10:0, C10:0 3-OH, C12:0, C14:0 and C18:1ω7c. Strain JC141(T) contained phosphatidylethanolamine and phosphatidylglycerol as major polar lipids, with minor amounts of diphosphatidylglycerol, unidentified aminolipids (AL1-3) and unidentified lipids (L1-4). A bacterial hopane derivative (BHD1) was the major hopanoid. Genomic DNA G+C content was 62.5 mol%. 16S rRNA gene sequence comparisons indicated that strain JC141(T) represents a member of the genus Vogesella within the family Neisseriaceae of the class Betaproteobacteria. Strain JC141(T) had a 16S rRNA gene sequence similarity of 98% with Vogesella indigofera ATCC 19706(T) and <96.5% with other members of the genus Vogesella. However, strain JC141(T) showed 45.8% relatedness (based on DNA-DNA hybridization) with V. indigofera LMG 6867(T) (=ATCC 19706(T)). Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of strain JC141(T) as a representative of a novel species in the genus Vogesella, for which the name Vogesella alkaliphila sp. nov. is proposed. The type strain is JC141(T) (=KCTC 32041(T)=LMG 27066(T)). An emended description of the genus Vogesella is also proposed.
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500 046, India.
authors.forename: Y
authors.initials: Y
authors.lastname: Subhash
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500 046, India.
authors.forename: L
authors.initials: L
authors.lastname: Tushar
authors.affiliation: Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J. N. T. University, Kukatpally, Hyderabad 500 085, India.
authors.forename: Ch
authors.initials: C
authors.lastname: Sasikala
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500 046, India.
authors.forename: Ch V
authors.initials: CV
authors.lastname: Ramana
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D007194:India; D008969:Molecular Sequence Data; D009346:Neisseriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014315:Triterpenes / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014315:Triterpenes; C025206:hopane