title: Interfering growth of malignant melanoma with Ang2-siRNA.
abstract: To investigate the intervention therapy effect on the growth of malignant melanoma, we have made an observation of expression levels of Ang2 in malignant melanoma cells, which was transduced by small interfering RNA (Ang2-siRNA) of Ang2 in vitro and in vivo. We successfully constructed Ang2-siRNA lent virus, and constructed nude mice model by transplanting malignant melanoma. Ang2-siRNA lent virus inhibited Ang2 mRNA of malignant melanoma in vitro and in vivo, and inhibited malignant melanoma tumor growth at the same time. Ang2-siRNA lent virus can interfere expression levels of Ang2 in malignant melanoma cells, inhibit tumor growth, and silent Ang2 gene expression, which may pave a new way for clinical gene therapy of malignant melanoma.
authors.affiliation: Department of Plastic Surgery, First Affiliated Hospital of Fujian Medical University, Road Chazhong 20, Taijiang, Fuzhou, 350005, China. wangbiaocn@126.com
authors.forename: Biao
authors.initials: B
authors.lastname: Wang
authors.forename: Zhaoliang
authors.initials: Z
authors.lastname: Liu
authors.forename: Mingfeng
authors.initials: M
authors.lastname: Zhang
authors.forename: Xiuying
authors.initials: X
authors.lastname: San
authors.forename: Yanding
authors.initials: Y
authors.lastname: Zhang
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Zhang
authors.forename: Meishui
authors.initials: M
authors.lastname: Wang
mesh_terms: D042702:Angiopoietin-2 / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D055785:Gene Knockdown Techniques; D015316:Genetic Therapy; D049452:Green Fluorescent Proteins / Q000096:biosynthesis; D057809:HEK293 Cells; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008545:Melanoma / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009368:Neoplasm Transplantation; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*; D017422:Sequence Analysis, DNA; D047368:Tumor Burden
chemical_list: D042702:Angiopoietin-2; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins