title: A putative transport protein is involved in citrulline excretion and re-uptake during arginine deiminase pathway activity by Lactobacillus sakei.
abstract: Arginine conversion through the arginine deiminase (ADI) pathway is a common metabolic trait of Lactobacillus sakei which is ascribed to an arc operon and which inquisitively involves citrulline excretion and re-uptake. The aim of this study was to verify whether a putative transport protein (encoded by the PTP gene) plays a role in citrulline-into-ornithine conversion by L. sakei strains. This was achieved through a combination of fermentation experiments, gene expression analysis via quantitative real-time reverse transcription PCR (RT-qPCR) and construction of a PTP knock-out mutant. Expression of the PTP gene was modulated by environmental pH and was highest in the end-exponential or mid-exponential growth phase for L. sakei strains CTC 494 and 23K, respectively. In contrast to known genes of the arc operon, the PTP gene showed low expression at pH 7.0, in agreement with the finding that citrulline-into-ornithine conversion is inhibited at this pH. The presence of additional energy sources also influenced ADI pathway activity, in particular by decreasing citrulline-into-ornithine conversion. Further insight into the functionality of the PTP gene was obtained with a knock-out mutant of L. sakei CTC 494 impaired in the PTP gene, which displayed inhibition in its ability to convert extracellular citrulline into ornithine. In conclusion, results indicated that the PTP gene may putatively encode a citrulline/ornithine antiporter.
authors.affiliation: Research Group of Industrial Microbiology and Food Biotechnology (IMDO), Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium. trimaux@vub.ac.be
authors.forename: Tom
authors.initials: T
authors.lastname: Rimaux
authors.forename: Audrey
authors.initials: A
authors.lastname: Rivière
authors.forename: Elvira María
authors.initials: EM
authors.lastname: Hebert
authors.forename: Fernanda
authors.initials: F
authors.lastname: Mozzi
authors.forename: Stefan
authors.initials: S
authors.lastname: Weckx
authors.forename: Luc
authors.initials: L
authors.lastname: De Vuyst
authors.forename: Frédéric
authors.initials: F
authors.lastname: Leroy
mesh_terms: D001120:Arginine / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002956:Citrulline / Q000378:metabolism*; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D055786:Gene Knockout Techniques; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000378:metabolism*; D007778:Lactobacillus / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D009952:Ornithine / Q000096:biosynthesis
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D002956:Citrulline; D001120:Arginine; D009952:Ornithine; D006867:Hydrolases; C019723:arginine deiminase