title: Mitochondrial Pathway of α-Tocopheryl Succinate-Induced Apoptosis in Human Epidermoid Carcinoma A431 Cells.
abstract: Vitamin E derivatives are known to act as agents exhibiting cytotoxity against tumor cells. The effect of vitamin E succinate on human epidermoid carcinoma cell line A431 was investigated in this study using live imaging, immunocytochemistry, and transmission electron microscopy. α-Tocopheryl succinate-induced apoptotic cell death in A431 cells was shown to be both dose- and time-dependent. The hyperproduction of reactive oxygen species, changes in size, shape and ultrastructural characteristics of mitochondria followed by the release of cytochromecfrom mitochondria to cytosol were observed. These results suggest that α-tocopheryl succinate induces apoptosis that occurs via the mitochondrial pathway. Mitochondria are shown to be crucial targets in α-tocopheryl succinate-induced caspase-dependent cell death in human carcinoma A431 cells.
authors.affiliation: Biological Faculty, Lomonosov Moscow State University, Leninskie gory, 1/12, Moscow, Russia, 119991.
authors.forename: M A
authors.initials: MA
authors.lastname: Savitskaya
authors.forename: M S
authors.initials: MS
authors.lastname: Vildanova
authors.forename: O P
authors.initials: OP
authors.lastname: Kisurina-Evgenieva
authors.forename: E A
authors.initials: EA
authors.lastname: Smirnova
authors.forename: G E
authors.initials: GE
authors.lastname: Onischenko