title: [Change of adhesion molecules in the lungs of rat with decompression sickness].
abstract: OBJECTIVE
  To investigate the change of adhesion molecules in the lungs of rats suffered with decompression sickness (DCS).
  
  
  METHODS
  Male SD rats were placed in the hyperbaric chamber, the chamber was compressed within 3 minutes to depths of 7 absolute atmosphere (ATA) and held at the designated depth for 60 min, then rapidly decompressed (3 min) to the surface. Rats were observed for signs of DCS after decompression. The brains, hepatis, and lungs were removed at 30 min, 6 h, 24 h post decompression, fixed and stained with hematoxylin eosin for routine histologic analysis. Lung paraffin sections were immunostained for the expression of intercellular adhesion molecule-1 (ICAM-1), E-selectin and major histocompatibility complex class II molecule (MHC-II). 2% evans blue dye in normal saline was injected 30 minutes prior to 6 h, 24 h before decompression. After 30 min, animals were perfused with 0.9% normal saline and lungs were harvested. Evans blue in the plasma was quantified by wavelength spectrophotometric analysis at 620 nm.
  
  
  RESULTS
  Results showed that there were hemorrhage and edema changes in the lungs, liver and brain at 30 min post decompression. Compared with control animals maintained at 1 ATA, the levels of E-selectin, ICAM-1 and MHC-II in the lungs of DCS rats were significantly increased post decompression. Compared with control animals, evans blue in the plasma was much higher at 6 h, 24 h post decompression.
  
  
  CONCLUSIONS
  The bubble-induced adhesion molecule-mediated endothelial activation may be involved in the pathogenesis of DCS.
authors.affiliation: Naval Medical Research Institute, Shanghai 200433, China.
authors.forename: Xiao-Chen
authors.initials: XC
authors.lastname: Bao
authors.forename: Yi-Qun
authors.initials: YQ
authors.lastname: Fang
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.forename: Miao
authors.initials: M
authors.lastname: Meng
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003665:Decompression Sickness / Q000378:metabolism*; D019040:E-Selectin / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism; D005802:Genes, MHC Class II; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008099:Liver / Q000473:pathology; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015815:Cell Adhesion Molecules; D019040:E-Selectin; D018799:Intercellular Adhesion Molecule-1