title: Vibrational spectroscopies for the analysis of cutaneous permeation: experimental limiting factors identified in the case of caffeine penetration.
abstract: Caffeine is utilised as a reference for permeation studies in dermatology and cosmetology. The present work aimed to monitor the permeation of a caffeine solution through the skin. For this purpose, Raman and infrared studies were performed. Raman microspectroscopy permitted a dynamic follow-up of the caffeine diffusion. In complementary, infrared microimaging provided information of the caffeine localization in the skin by applying multivariate statistical processing on skin tissue sections. Herein, we prove the possibility of tracking low concentrations of caffeine through the skin and we highlight some experimental limitations of vibrational spectroscopies.
authors.affiliation: MéDIAN Unit, CNRS FR3481, Faculty of Pharmacy, University of Reims Champagne-Ardenne (URCA), Reims, France. sanatfayli@hotmail.com
authors.forename: Sana
authors.initials: S
authors.lastname: Tfaili
authors.forename: Cyril
authors.initials: C
authors.lastname: Gobinet
authors.forename: Gwendal
authors.initials: G
authors.lastname: Josse
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Angiboust
authors.forename: Arlette
authors.initials: A
authors.lastname: Baillet
authors.forename: Michel
authors.initials: M
authors.lastname: Manfait
authors.forename: Olivier
authors.initials: O
authors.lastname: Piot
mesh_terms: D000279:Administration, Cutaneous; D002110:Caffeine / Q000008:administration & dosage / Q000032:analysis* / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D010539:Permeability; D012867:Skin / Q000737:chemistry / Q000378:metabolism*; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D002110:Caffeine