title: Sphingobacterium caeni sp. nov., isolated from activated sludge.
abstract: The taxonomic status of a bacterium, strain DC-8(T), isolated from activated sludge, was determined using a polyphasic taxonomic approach. The cells of strain DC-8(T) were Gram-negative, non-motile, non-spore-forming and rod-shaped. The isolate grew at temperature range of 10-40 °C (optimum 30-35 °C), pH range of 5.0-10.0 (optimum 6.5-8.0) and NaCl concentrations of 0-5% (optimum 0-1%). The predominant menaquinone of strain DC-8(T) was MK-7 and major fatty acids were summed feature 3 (C16:1ω6c and/or C16:1ω7c; 39.7%), iso-C15:0 (33.7%) and C16:0 (5.2%). The DNA G+C content was 39.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain DC-8(T) was a member of the genus Sphingobacterium. Strain DC-8(T) shared the highest similarity with Sphingobacterium siyangense SY1(T) (98.4%), Sphingobacterium multivorum IAM 14316(T) (98.3%), Sphingobacterium canadense CR11(T) (98.0%) and Sphingobacterium detergens 6.2S(T) (97.9%) and shared less than 97% similarity with other members of the genus Sphingobacterium. DNA-DNA hybridization experiments showed that the DNA-DNA relatedness values between strain DC-8(T) and its closest phylogenetic neighbours were below 70%. Based on the phylogenetic analysis, DNA-DNA hybridization, whole-cell fatty acid composition as well as biochemical characteristics, strain DC-8(T) was clearly distinguished from all recognized species of the genus Sphingobacterium and should be classified as a representative of a novel species of the genus Sphingobacterium, for which the name Sphingobacterium caeni sp. nov. is proposed. The type strain is DC-8(T) (=CCTCC AB 2012020(T)=KACC 16850(T)).
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Sun
authors.affiliation: College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: He
authors.affiliation: Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Shun-Peng
authors.initials: SP
authors.lastname: Li
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D045249:Sphingobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D024482:Vitamin K 2; C062629:menaquinone 7