title: Development of methods to distinguish between durum/common wheat and common wheat in blended flour using PCR.
abstract: A PCR-based method was developed to distinguish between durum/common wheat and common wheat by leveraging slight differences of DNA sequence in Starch Synthase II (SS II) coded on wheat A, B and D genomes. A primer pair, SS II ex7-U/L, was designed to hybridize with a conserved DNA sequence region found in SS II-A, B and D genes. Another primer pair, SS II-D 1769U/1889L, was constructed to recognize a unique sequence in the SS II-D gene. The target region of SS II ex7-U/L with the size of 114 bp was amplified from durum and common wheat DNA, while no amplification was observed from any cereals other than those in the wheat genus. A DNA fragment with the size of 121 bp was specifically amplified from common wheat with SS II-D 1769U/1889L. In blended flour prepared from wheat and other cereals, the developed PCR system composed of two primer pairs effectively detected durum/common wheat and common wheat. These results suggested that PCR using two primer pairs is useful for detecting common and/or durum wheat in blended flour and could be utilized to ensure accurate food labeling.
authors.affiliation: Central Laboratory, Nippon Flour Mills Co., Ltd, Kanagawa, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Matsuoka
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Arami
authors.forename: Megumi
authors.initials: M
authors.lastname: Sato
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Haraguchi
authors.forename: Youichi
authors.initials: Y
authors.lastname: Kurimoto
authors.forename: Shinjiro
authors.initials: S
authors.lastname: Imai
authors.forename: Keiko
authors.initials: K
authors.lastname: Tanaka
authors.forename: Junichi
authors.initials: J
authors.lastname: Mano
authors.forename: Satoshi
authors.initials: S
authors.lastname: Furui
authors.forename: Kazumi
authors.initials: K
authors.lastname: Kitta
mesh_terms: D001483:Base Sequence; D005433:Flour; D016133:Polymerase Chain Reaction / Q000379:methods*; D014908:Triticum / Q000235:genetics