title: The promoter of an A9 homolog from the conifer Cryptomeria japonica imparts male strobilus-dominant expression in transgenic trees.
abstract: KEY MESSAGE                 :                                     GUS analysis in                   Cryptomeria japonica                   revealed that the                   CjMALE1                   promoter is activated in the male strobilus of                   C. japonica.                                                 Toward the development of male sterile technology for Cryptomeria japonica, a male strobilus-dominant promoter of C. japonica was isolated. The CjMALE1 gene was isolated from a male strobilus-specific suppression subtractive hybridization (SSH) library, and the promoter was isolated by the TAIL-PCR method. To characterize the CjMALE1 promoter, β-glucuronidase (GUS)-fused genes were constructed and introduced into C. japonica using Agrobacterium tumefaciens. GUS expression from CjMALE1-2.5 K (2,718 bp fragment)::GUS                   C. japonica and CjMALE1-1 K (1,029 bp fragment)::GUS                   C. japonica was detected in the tapetum and microspore mother cells. These promoter fragments were comparably active in the pre-meiotic stage of the male strobilus of C. japonica. Our analysis showed that the 1,029 bp promoter had all the cis-elements necessary for male strobilus-dominant expression of CjMALE1. When CjMALE1-1 K::GUS was introduced into Arabidopsis, GUS expression was detected in the same spatiotemporal pattern as in C. japonica. These results suggest that the CjMALE1 promoter is subject to transcriptional regulatory systems consisting of cis- and trans-elements that have been highly conserved during evolution.
authors.affiliation: Forestry and Forest Products Research Institute, Forest Tree Breeding Center, 3809-1 Ishi, Juo, Hitachi, Ibaraki 319-1301, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Kurita
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Konagaya
authors.forename: Atsushi
authors.initials: A
authors.lastname: Watanabe
authors.forename: Teiji
authors.initials: T
authors.lastname: Kondo
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Ishii
authors.forename: Toru
authors.initials: T
authors.lastname: Taniguchi
mesh_terms: D016960:Agrobacterium tumefaciens; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics; D001483:Base Sequence; D032485:Cryptomeria / Q000166:cytology / Q000235:genetics*; D030561:Databases, Nucleic Acid; D035264:Flowers / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D005966:Glucuronidase; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D014197:Trees
chemical_list: D029681:Arabidopsis Proteins; D010940:Plant Proteins; C075574:A9 protein, Arabidopsis; D005966:Glucuronidase