title: Protocol for inducing flower color somaclonal variation in Torenia (Torenia fournieri Lind.).
abstract: White or light purple flower color Torenia (Torenia fournieri Lind.) varieties were successfully developed from the parental variety having violet flowers. This was accomplished by reducing Fe micronutrient in the culture media for the induction of in vitro flowering. The flower induction was highest in modified Murashige and Skoog (MS) medium containing ½ strength of macroelements, microelements, organic additives, and full Fe (M1) when compared to MS medium containing ½ strength of macronutrients, micronutrients, full Fe, and full organic additives (M2). The flower color was stable in two new Torenia varieties through three generations ex vitro. The results showed a wide range of somaclonal variation in flower colors; early flowering occurred in MS medium containing ½ strength of macroelements, microelements, Fe, and full strength of organic additives (M3). The selection of desirable somaclones and their micropropagation in subsequent generations led to the development of new and stable Torenia lines.
authors.affiliation: Tay Nguyen Institute of Biology, Hanoi, Vietnam.
authors.forename: Duong Tan
authors.initials: DT
authors.lastname: Nhut
authors.forename: Nguyen Thanh
authors.initials: NT
authors.lastname: Hai
authors.forename: Pham Thi Minh
authors.initials: PT
authors.lastname: Thu
authors.forename: Nguyen Ngoc
authors.initials: NN
authors.lastname: Thi
authors.forename: Truong Thi Dieu
authors.initials: TT
authors.lastname: Hien
authors.forename: Tran Trong
authors.initials: TT
authors.lastname: Tuan
authors.forename: Nguyen Ba
authors.initials: NB
authors.lastname: Nam
authors.forename: Nguyen Phuc
authors.initials: NP
authors.lastname: Huy
authors.forename: Hoang Xuan
authors.initials: HX
authors.lastname: Chien
authors.forename: Shri Mohan
authors.initials: SM
authors.lastname: Jain
mesh_terms: D000064:Acclimatization; D032461:Chromosomes, Plant / Q000235:genetics; D003470:Culture Media / Q000737:chemistry; D046508:Culture Techniques / Q000379:methods*; D005434:Flow Cytometry; D035264:Flowers / Q000033:anatomy & histology* / Q000254:growth & development; D010858:Pigmentation; D018520:Plant Shoots / Q000254:growth & development / Q000502:physiology; D011003:Ploidies; D028083:Scrophulariaceae / Q000033:anatomy & histology* / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D013242:Sterilization
chemical_list: D003470:Culture Media