title: Different anti-CD21 antibodies can be used to discriminate developmentally and functionally different subsets of B lymphocytes in circulation of pigs.
abstract: Monoclonal antibodies IAH-CC51, BB6-11C9.6 and B-Ly4 are routinely used to detect CD21 orthologue on the surface of porcine B lymphocytes. Cross-reactive studies show that IAH-CC51 and B-Ly4 recognize only a portion of B cells that are positive for pan-specific BB6-11C9.6. This indicates that CD21 is always present on all mature B cells but can be expressed in at least two differential forms, and these were assigned as CD21(a) and CD21(b). We used IAH-CC51 together with anti-CD2 to define four subpopulations of B cells. Ontogenetic and in vitro culture studies, analysis of cell size, expression of CD11b and class-switched phenotype together with measurement of proliferation and cell death, revealed that these subsets represent distinct populations. Phenotypic and functional features collectively suggest that CD21(b+) B cells are less mature than CD21(b-). The present work is the first to show that distinct subsets of mature B cells can express differential forms of CD21.
authors.affiliation: Department of Immunology and Gnotobiology, Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic, Doly 183, 54922 Novy Hradek, Czech Republic. Marek.Sinkora@worldonline.cz
authors.forename: Marek
authors.initials: M
authors.lastname: Sinkora
authors.forename: Katerina
authors.initials: K
authors.lastname: Stepanova
authors.forename: Jana
authors.initials: J
authors.lastname: Sinkorova
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D017209:Apoptosis; D016175:B-Lymphocyte Subsets / Q000145:classification / Q000276:immunology*; D002478:Cells, Cultured; D000939:Epitopes / Q000276:immunology; D017464:Receptors, Complement 3d / Q000276:immunology*; D013552:Swine / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D017464:Receptors, Complement 3d