title: [Changes in cardiac specific microRNA-208a level in peripheral blood in ST segment elevation acute myocardial infarction patients].
abstract: OBJECTIVE
  To observe serum cardiac specific microRNA-208a (miR-208a) levels in ST segment elevation acute myocardial infarction (STEAMI) patients, and to explore the role of serum miR-208a levels in the diagnosis of STEAMI.
  
  
  METHODS
  The serum miR-208a concentrations were assessed within 12 hours after STEAMI, while 30 healthy individuals as control. Serum miR-208a concentrations were measured with real-time quantity reverse transcription-polymerase chain reaction (qRT-PCR), and serum cardiac troponin I (cTnI) or MB isoenzyme of creatine kinase (CK-MB) concentrations were measured with enzyme linked immunosorbent assay (ELISA).
  
  
  RESULTS
  The contents of serum cTnI or CK-MB in STEAMI patients were significantly higher than those in healthy individuals (cTnI: 17.72±8.43 μg/L vs. 0.05±0.01 μg/L, CK-MB: 250.83±177.26 μg/L vs. 71.20±20.50 μg/L, both P<0.01). Serum miR-208a concentrations were detected in all individuals with STEAMI within 60 PCR cycle (0-6 hours: 44.95±4.77, 6-12 hours: 43.98±4.68), but were beyond detection for all individuals in the healthy control group. The serum miR-208a relative levels in STEAMI patients were at least more than 215 fold than that in healthy persons, compared with qRT-PCR (Ct=60) of miR-208a in healthy control persons (P<0.01).
  
  
  CONCLUSIONS
  Serum miR-208a may be a new biomarker the early diagnosis of STEAMI patients.
authors.affiliation: Department of Anesthesiology and Critical Care Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yao
authors.forename: Xian
authors.initials: X
authors.lastname: Zhou
authors.forename: Xiao-bin
authors.initials: XB
authors.lastname: Wang
authors.forename: Xiao-xia
authors.initials: XX
authors.lastname: Duan
authors.forename: Chun-xiang
authors.initials: CX
authors.lastname: Zhang
authors.forename: Xiao-ling
authors.initials: XL
authors.lastname: Shi
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Chen
authors.forename: Zhong-cai
authors.initials: ZC
authors.lastname: Fan
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D052279:Creatine Kinase, MB Form / Q000097:blood; D004562:Electrocardiography; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood* / Q000503:physiopathology*; D009206:Myocardium / Q000378:metabolism; D019210:Troponin I / Q000097:blood
chemical_list: C550906:MIRN208 microRNA, human; D035683:MicroRNAs; D019210:Troponin I; D052279:Creatine Kinase, MB Form