title: Genetic characterization by composite sequence analysis of a new pathogenic field strain of equine infectious anemia virus from the 2006 outbreak in Ireland.
abstract: Equine infectious anemia virus (EIAV), the causative agent of equine infectious anaemia (EIA), possesses the least-complex genomic organization of any known extant lentivirus. Despite this relative genetic simplicity, all of the complete genomic sequences published to date are derived from just two viruses, namely the North American EIAV(WYOMING) (EIAV(WY)) and Chinese EIAV(LIAONING) (EIAV(LIA)) strains. In 2006, an outbreak of EIA occurred in Ireland, apparently as a result of the importation of contaminated horse plasma from Italy and subsequent iatrogenic transmission to foals. This EIA outbreak was characterized by cases of severe, sometimes fatal, disease. To begin to understand the molecular mechanisms underlying this pathogenic phenotype, complete proviral genomic sequences in the form of 12 overlapping PCR-generated fragments were obtained from four of the EIAV-infected animals, including two of the index cases. Sequence analysis of multiple molecular clones produced from each fragment demonstrated the extent of diversity within individual viral genes and permitted construction of consensus whole-genome sequences for each of the four viral isolates. In addition, complete env gene sequences were obtained from 11 animals with differing clinical profiles, despite exposure to a common EIAV source. Although the overall genomic organization of the Irish EIAV isolates was typical of that seen in all other strains, the European viruses possessed ≤80 % nucleotide sequence identity with either EIAV(WY) or EIAV(LIA). Furthermore, phylogenetic analysis suggested that the Irish EIAV isolates developed independently of the North American and Chinese viruses and that they constitute a separate monophyletic group.
authors.affiliation: Virology Unit, Irish Equine Centre, Johnstown, Naas, Co. Kildare, Ireland.
authors.forename: Michelle
authors.initials: M
authors.lastname: Quinlivan
authors.affiliation: Gluck Equine Research Centre, Department of Veterinary Science, University of Kentucky, Lexington, KY 40545, USA.
authors.forename: Frank
authors.initials: F
authors.lastname: Cook
authors.affiliation: Virology Unit, Irish Equine Centre, Johnstown, Naas, Co. Kildare, Ireland.
authors.forename: Rachel
authors.initials: R
authors.lastname: Kenna
authors.affiliation: Veterinary Science Centre, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: John J
authors.initials: JJ
authors.lastname: Callinan
authors.affiliation: Virology Unit, Irish Equine Centre, Johnstown, Naas, Co. Kildare, Ireland.
authors.forename: Ann
authors.initials: A
authors.lastname: Cullinane
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004196:Disease Outbreaks / Q000662:veterinary*; D004859:Equine Infectious Anemia / Q000453:epidemiology / Q000821:virology*; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D016679:Genome, Viral; D006736:Horses; D004860:Infectious Anemia Virus, Equine / Q000235:genetics*; D007494:Ireland / Q000453:epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins