title: Reduced expression of LIMD1 in ulcerative oral epithelium associated with tobacco and areca nut.
abstract: OBJECTIVE
  The aim of this study was to cast light on initiating molecular events associated with the development of premalignant oral lesions induced by tobacco and/or areca nut.
  
  
  METHODS
  Immunohistochemical analyses of cell cycle regulatory proteins (LIMD1, RBSP3, p16, RB, phosphorylated RB, p53), EGFR and SH3GL2 (EGFR associated protein) were performed with inflammatory/ ulcerative epithelium and adjacent hyperplastic/mild dysplastic lesions.
  
  
  RESULTS
  No change in expression of the proteins was seen in inflammatory epithelium. Reduced nuclear expression of LIMD1 was evident in ulcerative epithelium. In hyperplastic lesions, reduced expression of RBSP3, p16, SH3GL2 and overexpression of p-RB and EGFR were apparent. Reduced nuclear expression of p53 was observed in mild dysplastic lesions.
  
  
  CONCLUSIONS
  Our data suggest that inactivation of LIMD1 in ulcerative epithelium might predispose the tissues to alterations of other cell cycle regulatory and EGFR signaling proteins needed for the development of premalignant oral lesions.
authors.affiliation: Department of Oncogene Regulation, Chittaranjan National Cancer Institute, India.
authors.forename: Guru Prasad
authors.initials: GP
authors.lastname: Maiti
authors.forename: Amlan
authors.initials: A
authors.lastname: Ghosh
authors.forename: Ramdas
authors.initials: R
authors.lastname: Chatterjee
authors.forename: Anup
authors.initials: A
authors.lastname: Roy
authors.forename: Tyson V
authors.initials: TV
authors.lastname: Sharp
authors.forename: Susanta
authors.initials: S
authors.lastname: Roychoudhury
authors.forename: Chinmay Kumar
authors.initials: CK
authors.lastname: Panda
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000328:Adult; D001114:Areca / Q000009:adverse effects; D002467:Cell Nucleus / Q000378:metabolism; D019941:Cyclin-Dependent Kinase Inhibitor p16; D066246:ErbB Receptors / Q000378:metabolism; D005260:Female; D006801:Humans; D006965:Hyperplasia / Q000378:metabolism; D007150:Immunohistochemistry; D007249:Inflammation / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D060588:LIM Domain Proteins / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000378:metabolism / Q000473:pathology*; D009363:Neoplasm Proteins / Q000378:metabolism; D019226:Oral Ulcer / Q000209:etiology / Q000378:metabolism*; D011230:Precancerous Conditions / Q000209:etiology / Q000378:metabolism*; D016160:Retinoblastoma Protein / Q000378:metabolism; D014030:Tobacco, Smokeless / Q000009:adverse effects; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000378:metabolism; D055815:Young Adult
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C000614131:CDKN2A protein, human; C486483:CTDSPL protein, human; D019941:Cyclin-Dependent Kinase Inhibitor p16; D047908:Intracellular Signaling Peptides and Proteins; D060588:LIM Domain Proteins; C403807:LIMD1 protein, human; D009363:Neoplasm Proteins; D016160:Retinoblastoma Protein; C107640:SH3GL2 protein, human; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins; D066246:ErbB Receptors