title: Generation of transgenic X. laevis models of retinal degeneration.
abstract: Transgenic models are invaluable tools for researching retinal degenerative disease mechanisms. However, they are time-consuming and expensive to generate and maintain. We have developed an alternative to transgenic rodent models of retinal degeneration using transgenic Xenopus laevis. We have optimized this system to allow rapid analysis of transgene effects in primary transgenic animals, thereby providing an alternative to establishing transgenic lines, and simultaneously allowing rigorous comparisons between the effects of different transgenes.
authors.affiliation: Department of Ophthalmology & Visual Sciences, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Beatrice M
authors.initials: BM
authors.lastname: Tam
authors.forename: Christine C-L
authors.initials: CC
authors.lastname: Lai
authors.forename: Zusheng
authors.initials: Z
authors.lastname: Zong
authors.forename: Orson L
authors.initials: OL
authors.lastname: Moritz
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D004195:Disease Models, Animal; D006801:Humans; D012162:Retinal Degeneration / Q000235:genetics*; D019076:Transgenes; D014982:Xenopus laevis / Q000235:genetics*