title: Antimicrobial effect of sophoraflavanone G isolated from Sophora flavescens against mutans streptococci.
abstract: In this study, the antibacterial properties of sophoraflavanone G isolated from the methanol extract of Sophora flavescens were tested against 16 strains of mutans streptococci to screen and determine the optimal concentration of anti-caries natural extract. The antimicrobial activity was evaluated by measuring minimum bactericidal concentration (MBC). The cell viability of normal human gingival fibroblast (NHGF) cells was tested using the methyl thiazolyl tetrazolium assay after exposure to sophoraflavanone G. The data showed that sophoraflavanone G had a remarkable antimicrobial effect on the bacteria tested with an MBC ranging from 0.5 μg/ml to 4 μg/ml. Sophoraflavanone G had no cytotoxic effect on NHGF cells at concentrations where it produced an antimicrobial effect. These findings demonstrate that sophoraflavanone G has strong antimicrobial activity against mutans streptococci and could be useful in the development of novel oral hygiene products, such as a gargle solution or dentifrice.
authors.affiliation: Department of Oral Biochemistry, School of Dentistry, Chosun University, 375 Seo-Suk Dong, Dong-Gu, Gwangju 501-759, Republic of Korea.
authors.forename: Chun Sung
authors.initials: CS
authors.lastname: Kim
authors.forename: Soon-Nang
authors.initials: SN
authors.lastname: Park
authors.forename: Sug-Joon
authors.initials: SJ
authors.lastname: Ahn
authors.forename: Young-Woo
authors.initials: YW
authors.lastname: Seo
authors.forename: Young-Ju
authors.initials: YJ
authors.lastname: Lee
authors.forename: Yun Kyong
authors.initials: YK
authors.lastname: Lim
authors.forename: Marcelo Oliveira
authors.initials: MO
authors.lastname: Freire
authors.forename: Eugene
authors.initials: E
authors.lastname: Cho
authors.forename: Joong-Ki
authors.initials: JK
authors.lastname: Kook
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects; D044950:Flavanones / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D006801:Humans; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D029909:Sophora / Q000737:chemistry*; D013291:Streptococcus / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D044950:Flavanones; C064849:vexibinol