title: Chemical optimization of protein extraction from sweet potato (Ipomoea batatas) peel.
abstract: Proteins isolated from sweet potatoes (Ipomoea batatas) have been shown to possess antidiabetic, antioxidant, and antiproliferative properties. The objective of this study was to chemically optimize a process for extracting proteins from sweet potato peel. The extraction procedure involved mixing peel with saline solvent to dissolve proteins and then precipitating with CaCl(2). Quadratic and segmented models were used to determine the optimum NaCl concentration and peel to solvent ratio to maximize protein solubility while minimizing solvent usage. A segmented model was also used to optimize the concentration of CaCl(2) used for precipitation. The highest yield was obtained by mixing blanched peelings with 59.7 mL of 0.025 mM NaCl per g peel and then precipitating with 6.8 mM CaCl(2). The results of this study show that potentially valuable proteins can be extracted from peel generated during processing of sweet potatoes and industrial costs can be minimized by using these optimum conditions.
  
  
  CONCLUSIONS
  Potentially valuable proteins can be extracted from sweet potato peel, a waste product of sweet potato processing.
authors.affiliation: Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, Box 7624, Raleigh, NC 27695, USA.
authors.forename: Katherine P
authors.initials: KP
authors.lastname: Maloney
authors.forename: Van-Den
authors.initials: VD
authors.lastname: Truong
authors.forename: Jonathan C
authors.initials: JC
authors.lastname: Allen
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D005511:Food Handling / Q000379:methods*; D027723:Ipomoea batatas / Q000737:chemistry*; D016014:Linear Models; D010940:Plant Proteins / Q000302:isolation & purification*
chemical_list: D010940:Plant Proteins