title: Identification of Blastocystis subtype 1 variants in the Home for Girls, Bangkok, Thailand.
abstract: A cross-sectional study of Blastocystis infection was conducted to evaluate the prevalence, risk factors, and subtypes of Blastocystis at the Home for Girls, Bangkok, Thailand in November 2008. Of 370 stool samples, 118 (31.9%) were infected with Blastocystis. Genotypic characterization of Blastocystis was performed by polymerase chain reaction and sequence analysis of the partial small subunit ribosomal RNA (SSU rRNA) gene. Subtype 1 was the most predominant (94.8%), followed by subtype 6 (3.5%) and subtype 2 (1.7%). Sequence analyses revealed nucleotide polymorphisms for Blastocystis subtype 1, which were described as subtype 1/variant 1, subtype 1/variant 2. Blastocystis subtype 1/variant 1 was the most predominant infection occurring in almost every house. The results showed that subtype analysis of Blastocystis was useful for molecular epidemiological study.
authors.affiliation: Department of Microbiology, Faculty of Science, Burapha University, Chonburi, Thailand. kungking65@hotmail.com
authors.forename: Umaporn
authors.initials: U
authors.lastname: Thathaisong
authors.forename: Suradej
authors.initials: S
authors.lastname: Siripattanapipong
authors.forename: Mathirut
authors.initials: M
authors.lastname: Mungthin
authors.forename: Duangnate
authors.initials: D
authors.lastname: Pipatsatitpong
authors.forename: Peerapan
authors.initials: P
authors.lastname: Tan-ariya
authors.forename: Tawee
authors.initials: T
authors.lastname: Naaglor
authors.forename: Saovanee
authors.initials: S
authors.lastname: Leelayoova
mesh_terms: D000293:Adolescent; D016844:Blastocystis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016776:Blastocystis Infections / Q000453:epidemiology* / Q000469:parasitology; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D060766:Drinking Water / Q000469:parasitology; D005191:Family Characteristics; D005243:Feces / Q000469:parasitology; D005260:Female; D005838:Genotype; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016053:RNA, Protozoan / Q000235:genetics / Q000302:isolation & purification*; D012335:RNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification*; D012307:Risk Factors; D017422:Sequence Analysis, DNA; D011795:Surveys and Questionnaires; D013785:Thailand; D055815:Young Adult
chemical_list: D060766:Drinking Water; D016053:RNA, Protozoan; D012335:RNA, Ribosomal