title: MicroRNA signatures in ischemia-reperfusion injury.
abstract: BACKGROUND
  MicroRNAs (miRs) are involved in gene expression and function, but little is known of their function in ischemia-reperfusion injury (IRI), which plays a critical role in flap compromise during microvascular flap surgeries. This article aimed to determine the expression profiles of miRs in rat flap surgeries after IRI.
  
  
  METHODS
  Vessel specimens in rat epigastric flap were initially determined for miRs survey after 2 hours of ischemia and 2 hours of reperfusion. Their miR expressions were further quantified after 2 hours of ischemia and subsequent reperfusion for 2 and 24 hours.
  
  
  RESULTS
  Only 3 miRs (miR-21, miR-193-3p, and miR-210) in rat vessels, but not in flap tissues, were significantly up-regulated at 24 hours of reperfusion after 2 hours of ischemia.
  
  
  CONCLUSIONS
  Our findings provide insights into deregulated expressions of miRs in flap surgeries after IRI. They might provide a further therapeutic consideration to prevent vascular compromise due to IRI in flap surgeries.
authors.affiliation: Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. kapich@kmu.edu.tw
authors.forename: Kao-Ping
authors.initials: KP
authors.lastname: Chang
authors.forename: Hsiao-Chen
authors.initials: HC
authors.lastname: Lee
authors.forename: Shu-Hung
authors.initials: SH
authors.lastname: Huang
authors.forename: Su-Shin
authors.initials: SS
authors.lastname: Lee
authors.forename: Chung-Sheng
authors.initials: CS
authors.lastname: Lai
authors.forename: Sin-Daw
authors.initials: SD
authors.lastname: Lin
mesh_terms: D000818:Animals; D019074:Epigastric Arteries; D005260:Female; D015870:Gene Expression; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008866:Microsurgery / Q000009:adverse effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000209:etiology / Q000235:genetics* / Q000601:surgery*; D013270:Stomach / Q000098:blood supply / Q000601:surgery; D013524:Surgical Flaps / Q000098:blood supply*; D015854:Up-Regulation; D014656:Vascular Surgical Procedures / Q000379:methods
chemical_list: C546388:MIRN210 microRNA, rat; D035683:MicroRNAs; C580762:Mirn193 microRNA, rat; C546082:mirn21 microRNA, rat