title: [The role of JNK in apoptosis of renal tubular epithelial cells in diabetic rats with fluctuant high blood glucose].
abstract: OBJECTIVE
  To explore the signal transduction mechanisms of apoptosis in renal tubular epithelial cells in diabetic rats with fluctuant high blood glucose.
  
  
  METHODS
  Healthy SD rats were randomly divided into 3 groups: normal control group (A), stable high blood glucose group (B) and fluctuant high blood glucose group (C). Diabetic rats were induced by intraperitoneal injection of streptozotocin (STZ, 65 mg/kg), and the fluctuant high blood glucose animal model was induced by intraperitoneal injection of ordinary insulin and glucose at different time point every day. The superoxide dismutase (SOD) activity and the content of malonaldehyde (MDA) in renal tissue homogenate were detected with colorimetry. The protein expression of Nox4 and JNK were examined by immunohistochemistry and Western blot. Apoptosis was assessed by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL).
  
  
  RESULTS
  After 12 experimental weeks, significantly increased cell apoptosis, up-regulation of Nox4 and P-JNK expression in renal tubular epithelial cells were observed in B and C groups compared with those in A group. The MDA content increased and SOD activity decreased in renal tissue in B and C groups. Above effects were more obviously shown in C group.
  
  
  CONCLUSIONS
  Fluctuant high blood glucose induced more apoptosis of renal tubular epithelial cell than stable high blood glucose in diabetic kidney, which might be related to the activation of JNK signal transduction pathway.
authors.affiliation: Department of Pathophysiology, the First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325035, China.
authors.forename: Mao-Lin
authors.initials: ML
authors.lastname: Hao
authors.forename: Yong-Yue
authors.initials: YY
authors.lastname: Dai
authors.forename: Shi-Rong
authors.initials: SR
authors.lastname: Ni
authors.forename: Da-Wang
authors.initials: DW
authors.lastname: Wang
authors.forename: Su-Juan
authors.initials: SJ
authors.lastname: Li
authors.forename: Ke-Ke
authors.initials: KK
authors.lastname: Jin
mesh_terms: D000818:Animals; D017209:Apoptosis; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology*; D004847:Epithelial Cells / Q000378:metabolism*; D007684:Kidney Tubules / Q000166:cytology; D048670:MAP Kinase Kinase 4 / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D000074663:NADPH Oxidase 4; D019255:NADPH Oxidases / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D000074663:NADPH Oxidase 4; D019255:NADPH Oxidases; C498911:Nox4 protein, rat; D048670:MAP Kinase Kinase 4