title: A bead-based suspension array for the serological detection of Trichinella in pigs.
abstract: The feasibility of using bead-based suspension arrays to detect serological evidence of Trichinella in pigs was assessed. Trichinella spiralis excretory-secretory antigen was covalently coupled to paramagnetic beads and used to bind serum antibodies, which were subsequently detected using anti-swine antibody. The assay was evaluated by testing pig sera from farms where trichinellosis was endemic and comparing the results with those obtained using two commercially available ELISAs. With cut-offs established by receiver operating characteristic (ROC) analysis, digestion-negative sera from a Trichinella-free population of pigs were deemed seronegative. When anti-swine antibody was replaced with protein A/G, higher test sensitivity (94% vs. 88%) at similar test specificity (95%), was achieved. The potential use of this assay in species other than swine was also demonstrated by testing human sera.
authors.affiliation: Central Veterinary Institute, Wageningen UR, Lelystad, The Netherlands. fimme.vanderwal@wur.nl
authors.forename: F J
authors.initials: FJ
authors.lastname: van der Wal
authors.forename: R P
authors.initials: RP
authors.lastname: Achterberg
authors.forename: A
authors.initials: A
authors.lastname: Kant
authors.forename: C B M
authors.initials: CB
authors.lastname: Maassen
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000175:diagnosis* / Q000469:parasitology; D014234:Trichinella / Q000302:isolation & purification*; D014235:Trichinellosis / Q000097:blood / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*