title: Kinetic analysis of Arabidopsis glucosyltransferase UGT74B1 illustrates a general mechanism by which enzymes can escape product inhibition.
abstract: Plant genomes encode numerous small molecule glycosyltransferases which modulate the solubility, activity, immunogenicity and/or reactivity of hormones, xenobiotics and natural products. The products of these enzymes can accumulate to very high concentrations, yet somehow avoid inhibiting their own biosynthesis. Glucosyltransferase UGT74B1 (UDP-glycosyltransferase 74B1) catalyses the penultimate step in the core biosynthetic pathway of glucosinolates, a group of natural products with important functions in plant defence against pests and pathogens. We found that mutation of the highly conserved Ser284 to leucine [wei9-1 (weak ethylene insensitive)] caused only very mild morphological and metabolic phenotypes, in dramatic contrast with knockout mutants, indicating that steady state glucosinolate levels are actively regulated even in unchallenged plants. Analysis of the effects of the mutation via a structural modelling approach indicated that the affected serine interacts directly with UDP-glucose, but also predicted alterations in acceptor substrate affinity and the kcat value, sparking an interest in the kinetic behaviour of the wild-type enzyme. Initial velocity and inhibition studies revealed that UGT74B1 is not inhibited by its glycoside product. Together with the effects of the missense mutation, these findings are most consistent with a partial rapid equilibrium ordered mechanism. This model explains the lack of product inhibition observed both in vitro and in vivo, illustrating a general mechanism whereby enzymes can continue to function even at very high product/precursor ratios.
authors.affiliation: Department of Molecular Signal Processing, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle (Saale), Germany.
authors.forename: Jakub
authors.initials: J
authors.lastname: Kopycki
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Wieduwild
authors.forename: Janine
authors.initials: J
authors.lastname: Kohlschmidt
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Brandt
authors.forename: Anna N
authors.initials: AN
authors.lastname: Stepanova
authors.forename: Jose M
authors.initials: JM
authors.lastname: Alonso
authors.forename: M Soledade C
authors.initials: MS
authors.lastname: Pedras
authors.forename: Steffen
authors.initials: S
authors.lastname: Abel
authors.forename: C Douglas
authors.initials: CD
authors.lastname: Grubb
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000378:metabolism; D018745:Genome, Plant; D005964:Glucosyltransferases / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D007930:Leucine / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D009154:Mutation; D010641:Phenotype; D030821:Plants, Genetically Modified; D012694:Serine / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D012694:Serine; D005964:Glucosyltransferases; C499436:UDP-glucose thiohydroximate S-glucosyltransferase UGT74B1, Arabidopsis; D007930:Leucine; D014532:Uridine Diphosphate Glucose