title: Extracellular histones inhibit hair shaft elongation in cultured human hair follicles and promote regression of hair follicles in mice.
abstract: Release of histone H4 in rat vibrissa dermal papilla (DP) cells exposed to sub-toxic dose of colchicines has been recently reported. In addition, exposure to histone H4 has been reported to result in inhibited proliferation and reduced alkaline phosphatase (ALP) activity of cultured vibrissa DP cells. These findings prompted us to investigate the role of extracellular histones in hair growth using cultured human hair follicles and hair cycling using back skin of mice. We report here that exposure of cultured hair follicles to histone H4 and H2A resulted in significant inhibition of elongation of hair shafts, decreased expression of IGF-1 and decreased expression and activity of ALP. Injection of histones into hypodermis of mice during anagen resulted in premature onset of catagen. Findings of the current study provide strong evidence suggesting the inhibitory role of extracellular histones in hair growth.
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Shin
authors.forename: Hyun Woo
authors.initials: HW
authors.lastname: Joo
authors.forename: Moon Kyu
authors.initials: MK
authors.lastname: Kim
authors.forename: Jung Chul
authors.initials: JC
authors.lastname: Kim
authors.forename: Young Kwan
authors.initials: YK
authors.lastname: Sung
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D006197:Hair / Q000187:drug effects* / Q000254:growth & development; D018859:Hair Follicle / Q000187:drug effects* / Q000254:growth & development / Q000473:pathology; D006657:Histones / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D006657:Histones; D011994:Recombinant Proteins