title: Gibberellins regulate the transcription of the continuous flowering regulator, RoKSN, a rose TFL1 homologue.
abstract: The role of gibberellins (GAs) during floral induction has been widely studied in the annual plant Arabidopsis thaliana. Less is known about this control in perennials. It is thought that GA is a major regulator of flowering in rose. In spring, low GA content may be necessary for floral initiation. GA inhibited flowering in once-flowering roses, whereas GA did not block blooming in continuous-flowering roses. Recently, RoKSN, a homologue of TFL1, was shown to control continuous flowering. The loss of RoKSN function led to continuous flowering behaviour. The objective of this study was to understand the molecular control of flowering by GA and the involvement of RoKSN in this inhibition. In once-flowering rose, the exogenous application of GA(3) in spring inhibited floral initiation. Application of GA(3) during a short period of 1 month, corresponding to the floral transition, was sufficient to inhibit flowering. At the molecular level, RoKSN transcripts were accumulated after GA(3) treatment. In spring, this accumulation is correlated with floral inhibition. Other floral genes such as RoFT, RoSOC1, and RoAP1 were repressed in a RoKSN-dependent pathway, whereas RoLFY and RoFD repression was RoKSN independent. The RoKSN promoter contained GA-responsive cis-elements, whose deletion suppressed the response to GA in a heterologous system. In summer, once-flowering roses did not flower even after exogenous application of a GA synthesis inhibitor that failed to repress RoKSN. A model is presented for the GA inhibition of flowering in spring mediated by the induction of RoKSN. In summer, factors other than GA may control RoKSN.
authors.affiliation: INRA, Institut de Recherche en Horticulture et Semences (INRA, Agrocacmpus-Ouest, Université d'Angers), SFR 4207 QUASAV, BP 60057, 49071 Beaucouzé Cedex,  France.
authors.forename: Marie
authors.initials: M
authors.lastname: Randoux
authors.forename: Julien
authors.initials: J
authors.lastname: Jeauffre
authors.forename: Tatiana
authors.initials: T
authors.lastname: Thouroude
authors.forename: François
authors.initials: F
authors.lastname: Vasseur
authors.forename: Latifa
authors.initials: L
authors.lastname: Hamama
authors.forename: Marjorie
authors.initials: M
authors.lastname: Juchaux
authors.forename: Soulaiman
authors.initials: S
authors.lastname: Sakr
authors.forename: Fabrice
authors.initials: F
authors.lastname: Foucher
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D060405:Florigen / Q000378:metabolism* / Q000494:pharmacology; D035264:Flowers / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant / Q000187:drug effects; D005875:Gibberellins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018613:Microscopy, Confocal; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000187:drug effects; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D027826:Rosa / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D012621:Seasons; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000235:genetics; D015854:Up-Regulation
chemical_list: D060405:Florigen; D005875:Gibberellins; D018749:RNA, Plant; D049452:Green Fluorescent Proteins