title: MiR-125b inhibits tumor growth and promotes apoptosis of cervical cancer cells by targeting phosphoinositide 3-kinase catalytic subunit delta.
abstract: BACKGROUND
  [corrected] microRNAs (miRNAs) are involved in cancer-related processes. The miRNA-125b (miR-125b) has been identified as miRNA over-expressed in a wide variety of cancers. However, the role of miR-125b in the context of cervical carcinoma remains unknown.
  
  
  METHODS
  In this study, the effect of miR-125b on the proliferation and apoptosis of human cervical cells was analyzed by MTT assay and Flow cytometry analysis. we identified phosphoinositide 3-kinase catalytic subunit delta (PIK3CD) as a novel miR-125b target.
  
  
  RESULTS
  overexpression of miR- 125b in HeLa cervical cancer cells decreased cell proliferation, induced apoptosis and down-regulated expression of PIK3CD. To identify the mechanisms responsible, we investigated the PI3K/Akt pathway and found that PI3K, phospho-Akt, and phospho-mTOR were all down-regulated, while Bid was up-regulated in miR-125b-overexpressing subclones. In vivo, over expression of miR-125b in HeLa cells markedly reduced their ability to form tumors.
  
  
  CONCLUSIONS
  these results suggest that miR-125b suppresses tumor growth activity by targeting the PI3K/ Akt/mTOR signaling pathway, and may provide a target for effective therapies.
authors.affiliation: Department of Gynaecology and Obstetric Medicine, Shanghai Jiangwan Hospital, Shanghai, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Cui
authors.forename: Xiuli
authors.initials: X
authors.lastname: Li
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Zhu
authors.forename: Lili
authors.initials: L
authors.lastname: Huang
authors.forename: Yongfang
authors.initials: Y
authors.lastname: Huang
authors.forename: Caiying
authors.initials: C
authors.lastname: Mao
authors.forename: Qi
authors.initials: Q
authors.lastname: Yan
authors.forename: Jianhong
authors.initials: J
authors.lastname: Zhu
authors.forename: Wenxia
authors.initials: W
authors.lastname: Zhao
authors.forename: Hong
authors.initials: H
authors.lastname: Shi
mesh_terms: D000970:Antineoplastic Agents; D017209:Apoptosis; D049109:Cell Proliferation; D058534:Class I Phosphatidylinositol 3-Kinases; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D019869:Phosphatidylinositol 3-Kinases / Q000096:biosynthesis / Q000235:genetics*; D013329:Structure-Activity Relationship; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology*
chemical_list: D000970:Antineoplastic Agents; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D019869:Phosphatidylinositol 3-Kinases; D058534:Class I Phosphatidylinositol 3-Kinases; C486018:PIK3CD protein, human