title: Sugar-induced endocytosis of plant 7TM-RGS proteins.
abstract: Plant cells use sugars mainly as a source or store of energy and carbon skeletons for anabolic reactions and for osmotic regulation. The perception of sugars and their responses are rather complex including the heterotrimeric G protein pathway and a seven-transmembrane RGS molecule. Previously, we found that endocytosis of the 7TM-RGS leads to sustained activation of the G protein pathway in the genetic model Arabidopsis. Here we show that other plants possess similar endocytosis systems of the 7TM-RGS proteins. A phosphorylation site essential for the endocytosis is well conserved in land plant 7TM-RGS proteins. In addition, conifer and tobacco 7TM-RGS proteins are internalized in response to sugar. These results indicate a universal mechanism to activate G signaling by endocytosis in plant cells that have 7TM-RGS proteins.
authors.affiliation: Department of Biology; University of North Carolina at Chapel Hill; Chapel Hill, NC USA.
authors.forename: Nguyen
authors.initials: N
authors.lastname: Phan
authors.affiliation: Department of Biology; University of North Carolina at Chapel Hill; Chapel Hill, NC USA.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Urano
authors.affiliation: Department of Experimental Plant Biology; Faculty of Science; Charles University in Prague; Prague, Czech Republic.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Srba
authors.affiliation: Department of Experimental Plant Biology; Faculty of Science; Charles University in Prague; Prague, Czech Republic.
authors.forename: Lukas
authors.initials: L
authors.lastname: Fischer
authors.affiliation: Department of Biology; University of North Carolina at Chapel Hill; Chapel Hill, NC USA; Department of Pharmacology; University of North Carolina at Chapel Hill; Chapel Hill, NC USA.
authors.forename: Alan M
authors.initials: AM
authors.lastname: Jones
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D004705:Endocytosis / Q000187:drug effects* / Q000235:genetics; D005947:Glucose / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D020710:RGS Proteins / Q000378:metabolism*; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D064028:Tracheophyta / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D010940:Plant Proteins; D020710:RGS Proteins; D005947:Glucose