title: Borate enhances the production of lactulose from lactose by cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus.
abstract: Cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus was used in the presence of borate to increase the production of lactulose from lactose. Maximum production of lactulose occurred using a 1:1M ratio of borate-lactose. Under this condition, the enzyme produced 614 g l(-1) lactulose from 700 g l(-1) lactose after incubation at pH 7.5 and 80 °C for 3h, with a conversion yield of 88% and a productivity of 205 g l(-1) h(-1). The yield and productivity of lactulose production obtained in the present study are among the highest achieved through chemical or biological synthesis.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Yeong-Su
authors.initials: YS
authors.lastname: Kim
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Kim
authors.forename: Deok-Kun
authors.initials: DK
authors.lastname: Oh
mesh_terms: D001881:Borates / Q000737:chemistry*; D002238:Carbohydrate Epimerases / Q000737:chemistry*; D007785:Lactose / Q000138:chemical synthesis*; D007792:Lactulose / Q000737:chemistry*; D046968:Thermoanaerobacter / Q000201:enzymology*
chemical_list: D001881:Borates; D007792:Lactulose; D002238:Carbohydrate Epimerases; D007785:Lactose