title: +276 G/T single nucleotide polymorphism of the adiponectin gene is associated with the susceptibility to biliary atresia.
abstract: BACKGROUND
  Biliary atresia (BA) is an intractable neonatal inflammatory and obliterative cholangiopathy, leading to progressive fibrosis and cirrhosis. Adiponectin, an anti-inflammatory adipokine, is known to play a possible role in liver diseases. The objective of our study was to determine the relationship between adiponectin gene polymorphisms and BA susceptibility.
  
  
  METHODS
  A total of 106 BA patients and 107 healthy controls were included in this study. Two single nucleotide polymorphisms (SNPs) of the adiponectin gene, +45T/G (rs2241766) and +276G/T (rs1501299), were genotyped by polymerase chain reaction-restriction fragment length polymorphism analysis.
  
  
  RESULTS
  Genotype distributions of +45 T/G and +276 G/T SNPs were seen in the Hardy-Weinberg equilibrium for both BA patients and controls. The frequency of the G/G genotype at +276G/T was significantly higher in BA patients than in the controls (P=0.009). Regarding +45T/ G in BA patients, the frequency of the T/T genotype tended to be lower than in the controls, but the difference was not significant. Moreover, the G allele at +276G/T in BA patients was more common than in the controls (P=0.0043). In contrast, the frequency of the T allele at +45T/G was not significantly different between BA patients and the controls. None of the haplotypes studied was found to significantly influence the risk of BA.
  
  
  CONCLUSIONS
  +276G/T SNP is strongly associated with BA, particularly with the G allele. We postulate that the +276G/T adiponectin gene polymorphism confers increased susceptibility to BA.
authors.affiliation: Program in Medical Sciences, Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand.
authors.forename: Wanvisa
authors.initials: W
authors.lastname: Udomsinprasert
authors.forename: Tewin
authors.initials: T
authors.lastname: Tencomnao
authors.forename: Sittisak
authors.initials: S
authors.lastname: Honsawek
authors.forename: Wilai
authors.initials: W
authors.lastname: Anomasiri
authors.forename: Paisarn
authors.initials: P
authors.lastname: Vejchapipat
authors.forename: Voranush
authors.initials: V
authors.lastname: Chongsrisawat
authors.forename: Yong
authors.initials: Y
authors.lastname: Poovorawan
mesh_terms: D052242:Adiponectin / Q000235:genetics*; D000293:Adolescent; D044466:Asian People / Q000235:genetics; D001656:Biliary Atresia / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide; D013785:Thailand
chemical_list: D052242:Adiponectin