title: Immunomodulatory/anti-inflammatory effects of Baccharis dracunculifolia leaves.
abstract: A possible immunomodulatory/anti-inflammatory effect of Baccharis dracunculifolia (Bd) and its major compound--caffeic acid (Ca)--on cytokines production (IL-1β, IL-6 and IL-10) by murine macrophages was investigated. Cells were incubated with Bd and Ca, and the inhibitory concentrations were tested before or after macrophages challenge with LPS. Bd and Ca stimulated IL-1β and inhibited IL-6 and IL-10 production. In LPS-challenge protocols, Bd prevented LPS action either before or after LPS challenge, whereas Ca prevented LPS effects only after LPS addition. Bd modulatory action on cytokines production may be at least in part mediated by Ca, since it has been shown to inhibit the transcription factor NF-κB. Further studies are still needed to evaluate Bd efficacy in inflammatory diseases, in order to explore its antiinflammatory activity in vivo.
authors.affiliation: a Department of Microbiology and Immunology , Biosciences Institute, UNESP , Botucatu , SP , 18618-970 , Brazil.
authors.forename: T F
authors.initials: TF
authors.lastname: Bachiega
authors.forename: J P B
authors.initials: JP
authors.lastname: de Sousa
authors.forename: J K
authors.initials: JK
authors.lastname: Bastos
authors.forename: J M
authors.initials: JM
authors.lastname: Sforcin
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000494:pharmacology*; D031175:Baccharis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D007155:Immunologic Factors / Q000737:chemistry* / Q000494:pharmacology*; D016753:Interleukin-10 / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D007155:Immunologic Factors; D053583:Interleukin-1beta; D015850:Interleukin-6; D016328:NF-kappa B; D016753:Interleukin-10