title: In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies.
abstract: The dopamine receptor D2 (DRD2), a G-protein coupled receptor is expressed into PBd(22)-PEO(13) and PMOXA(20)-PDMS(54)-PMOXA(20) block copolymer vesicles. The conformational integrity of the receptor is confirmed by antibody- and ligand-binding assays. Replacement of bound dopamine is demonstrated on surface-immobilized polymersomes, thus making this a promising platform for drug screening.
authors.affiliation: Patterning and Fabrication, Institute of Materials Research and Engineering, Agency for Science, Technology and Research, Singapore, Singapore.
authors.forename: Sylvia
authors.initials: S
authors.lastname: May
authors.forename: Mirjam
authors.initials: M
authors.lastname: Andreasson-Ochsner
authors.forename: Zhikang
authors.initials: Z
authors.lastname: Fu
authors.forename: Ying Xiu
authors.initials: YX
authors.lastname: Low
authors.forename: Darren
authors.initials: D
authors.lastname: Tan
authors.forename: Hans-Peter M
authors.initials: HP
authors.lastname: de Hoog
authors.forename: Sandra
authors.initials: S
authors.lastname: Ritz
authors.forename: Madhavan
authors.initials: M
authors.lastname: Nallani
authors.forename: Eva-Kathrin
authors.initials: EK
authors.lastname: Sinner
mesh_terms: D055808:Drug Discovery; D006801:Humans; D008024:Ligands; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D008024:Ligands; D011108:Polymers; D043562:Receptors, G-Protein-Coupled