title: Phyllocladane diterpenes from Anisomeles heyneana.
abstract: New phyllocladane diterpene, phyllocladan-16α,17-dihydroxy-19-oic acid (1), together with known phyllocladane diterpene, phyllocladan-16α,19-diol (2), cembrane diterpene ovatodiolide (3), sitosteryl-3-O-β-D-glucoside (4), and verbascoside (5), were isolated from aerial parts of Anisomeles heyneana. The structure of compound 1 was elucidated by 1D and 2D NMR analyses which included HSQC, HMBC, and nuclear overhauser effect spectroscopy (NOESY) experiments as well as X-ray crystallography. This is the first report of phyllocladane diterpenes from genus Anisomeles. Compounds 1, 3, 4, and 5 were evaluated for inhibition of Mycobacterium tuberculosis and 3 was found to exhibit anti-mycobacterial activity with IC₉₀ 6.53 μg/ml. Compounds 1, 3, and 5, at 100 μg/ml, were also evaluated for inhibition of Thp-1 cell lines, and compounds 1 and 3 showed 59.02% and 96.4% inhibitions, respectively.
authors.affiliation: Divison of Organic Chemistry, National Chemical Laboratory, Pune 411008, India.
authors.forename: Roshan R
authors.initials: RR
authors.lastname: Kulkarni
authors.forename: Ketaki
authors.initials: K
authors.lastname: Shurpali
authors.forename: Rupesh L
authors.initials: RL
authors.lastname: Gawde
authors.forename: Dhiman
authors.initials: D
authors.lastname: Sarkar
authors.forename: Vedavati G
authors.initials: VG
authors.lastname: Puranik
authors.forename: Swati P
authors.initials: SP
authors.lastname: Joshi
mesh_terms: D000995:Antitubercular Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007194:India; D019686:Lamiaceae / Q000737:chemistry*; D015394:Molecular Structure; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D019906:Nuclear Magnetic Resonance, Biomolecular; D013237:Stereoisomerism
chemical_list: D000995:Antitubercular Agents; D004224:Diterpenes; D005960:Glucosides; C578217:phyllocladan-16alpha,17-dihydroxy-19-oic acid