title: In vitro propagation of peanut (Arachis hypogaea L.) by shoot tip culture.
abstract: Peanut (Arachis hypogaea L.), also known as groundnut, is the most important species of Arachis genus, originating from Brazil and Peru. Peanut seeds contain high seed oil, proteins, amino acids, and vitamin E, and are consumed worldwide as edible nut, peanut butter, or candy, and peanut oil extracted from the seeds. The meal remaining after oil extraction is also used for animal feed. However, its narrow germplasm base, together with susceptibility to diseases, pathogens, and weeds, decreases yield and seed quality and causes great economic losses annually. Hence, the optimization of efficient in vitro propagation procedures would be highly effective for peanut propagation, as it would raise yield and improve seed quality and flavor. Earlier reports on traditional micropropagation methods, based on axillary bud proliferation which guarantees the multiplication of true-to-type plants, are still limited. This chapter describes a micropropagation protocol to improve multiple shoot formation from shoot-tip explants by using AgNO(3) in combination with plant growth regulators.
authors.affiliation: IVALSA-Istituto per la Valorizzazione del Legno e delle Specie Arboree, National Research Council (CNR), Sesto Fiorentino, Florence, Italy. elifaylinozudogru@yahoo.it
authors.forename: Elif Aylin
authors.initials: EA
authors.lastname: Ozudogru
authors.forename: Ergun
authors.initials: E
authors.lastname: Kaya
authors.forename: Maurizio
authors.initials: M
authors.lastname: Lambardi
mesh_terms: D000064:Acclimatization; D010367:Arachis / Q000254:growth & development* / Q000502:physiology; D003470:Culture Media / Q000737:chemistry; D046508:Culture Techniques / Q000379:methods*; D018525:Germination; D018517:Plant Roots / Q000254:growth & development / Q000502:physiology; D018520:Plant Shoots / Q000254:growth & development* / Q000502:physiology; D012639:Seeds / Q000254:growth & development / Q000502:physiology; D013242:Sterilization
chemical_list: D003470:Culture Media