title: HIV-1 infection-induced apoptotic microparticles inhibit human DCs via CD44.
abstract: Acute HIV-1 infection results in dysregulated immunity, which contributes to poor control of viral infection. DCs are key regulators of both adaptive and innate immune responses needed for controlling HIV-1, and we surmised that factors elicited during acute HIV-1 infection might impede DC function. We derived immature DCs from healthy donor peripheral blood monocytes and treated them with plasma from uninfected control donors and donors with acute HIV-1 infections. We found that the plasma from patients with HIV specifically inhibited DC function. This suppression was mediated by elevated apoptotic microparticles derived from dying cells during acute HIV-1 infection. Apoptotic microparticles bound to and inhibited DCs through the hyaluronate receptor CD44. These data suggest that targeting this CD44-mediated inhibition by apoptotic microparticles could be a novel strategy to potentiate DC activation of HIV-specific immunity.
authors.affiliation: New York University Langone Medical Center, New York, New York 10016, USA.
authors.forename: Davor
authors.initials: D
authors.lastname: Frleta
authors.forename: Carolyn E
authors.initials: CE
authors.lastname: Ochoa
authors.forename: Holger B
authors.initials: HB
authors.lastname: Kramer
authors.forename: Shaukat Ali
authors.initials: SA
authors.lastname: Khan
authors.forename: Andrea R
authors.initials: AR
authors.lastname: Stacey
authors.forename: Persephone
authors.initials: P
authors.lastname: Borrow
authors.forename: Benedikt M
authors.initials: BM
authors.lastname: Kessler
authors.forename: Barton F
authors.initials: BF
authors.lastname: Haynes
authors.forename: Nina
authors.initials: N
authors.lastname: Bhardwaj
mesh_terms: D017209:Apoptosis; D055252:Cell-Derived Microparticles / Q000276:immunology* / Q000821:virology; D003713:Dendritic Cells / Q000276:immunology* / Q000502:physiology / Q000821:virology; D015658:HIV Infections / Q000097:blood / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology* / Q000502:physiology; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism* / Q000502:physiology; D007113:Immunity, Innate; D051193:Toll-Like Receptors / Q000378:metabolism; D014766:Viremia / Q000821:virology
chemical_list: C497483:CD44 protein, human; D018960:Hyaluronan Receptors; D051193:Toll-Like Receptors