title: MLVA-16 loci panel on Brucella spp. using multiplex PCR and multicolor capillary electrophoresis.
abstract: The Multi Locus Variable Number Tandem Repeat Analysis 16 loci panel (MLVA-16), involving singleplex PCRs and agarose gel electrophoresis, is the standard genotyping method for Brucella spp., used also for the Brucella international online database. We describe an alternative, reliable, high-throughput MLVA-16 protocol using multiplex PCRs and multicolor capillary electrophoresis.
authors.affiliation: Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale - National and OIE Reference Laboratory for Brucellosis, Italy. g.garofolo@izs.it
authors.forename: Giuliano
authors.initials: G
authors.lastname: Garofolo
authors.forename: Massimo
authors.initials: M
authors.lastname: Ancora
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Di Giannatale
mesh_terms: D002002:Brucella / Q000145:classification* / Q000235:genetics*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D018598:Minisatellite Repeats; D058889:Molecular Typing / Q000379:methods; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*