title: A novel strain D5 isolated from Acacia confusa.
abstract: We isolated a novel strain D5 from nodules of Acacia confusa. Under strict sterile conditions the strain could successfully nodulate Acacia confusa, A. crassicarpa and A. mangium, with nitrogenase activity ranging from 18.90 to 19.86 nmol·g(-1)·min(-1). In the phylogenetic tree based on a complete 16S rRNA gene sequence, the sequence of strain D5 shared 99% homology with that of four species of genus Pseudomonas. The 685 bp nodA fragment amplified from strain D5 shared 95% homology with the nodA sequence of 9 species of genus Bradyrhizobium, with a genetic distance of 0.01682. The 740 bp nifH gene fragment was amplified from strain D5. This strain D5 nifH gene and Bradyrhizobium spp. formed a branch, showing 98% homology and a genetic distance of 0. The homology between this branch and the Bradyrhizobium spp. DG in another branch was 99%, with a genetic distance of 0.007906. These results indicate that this strain D5 is a new type of nitrogen-fixing bacterium.
authors.affiliation: Forestry College, Guangxi University, Nanning, China. hnboln@126.com
authors.forename: Baoling
authors.initials: B
authors.lastname: Huang
authors.forename: Chengqun
authors.initials: C
authors.lastname: Lv
authors.forename: Yili
authors.initials: Y
authors.lastname: Zhao
authors.forename: Rong
authors.initials: R
authors.lastname: Huang
mesh_terms: D000045:Acacia / Q000382:microbiology*; D001483:Base Sequence; D020369:Bradyrhizobium / Q000235:genetics* / Q000302:isolation & purification; D002681:China; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D019143:Evolution, Molecular; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009591:Nitrogenase / Q000378:metabolism; D010802:Phylogeny; D055170:Plant Root Nodulation / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D012336:RNA, Ribosomal, 16S; D009591:Nitrogenase