title: Genotyping of human papillomavirus in paraffin embedded cervical tissue samples from women in Ethiopia and the Sudan.
abstract: Cervical cancer is the most frequent female malignancy in most developing countries. Previous studies have demonstrated a strong association of human papillomavirus (HPV) infection with dysplasia and carcinoma of the uterine cervix. The objective of this study was to identify the prevailing HPV genotypes responsible for the development of cervical cancer among women in Ethiopia and the Sudan. A molecular characterization of HPV was done on 245 paraffin embedded cervical biopsy samples collected from the two countries. Amplification of HPV and subsequent genotyping was done using SPF10 primers and Line probe assay. Of samples collected from Ethiopian patients, 93% (149/160) and 13% (21/160) had high risk and low risk HPV genotypes, respectively. Among samples collected from the Sudan, 94% (80/85) harbored high risk and 11.7% (10/85) low risk HPV genotypes. Human papillomavirus 16 was the most frequent genotype identified in samples from Ethiopia (91%, 136/149) and the Sudan (82.5%, 66/80). HPV 52, 58, and 18 were the second, third and fourth common genotypes identified in Ethiopia, whereas HPV 18, 45, and 52 were the second, third, and fourth genotypes identified in samples collected from the Sudan. Thus, individuals living in different geographical localities should receive vaccines based on the specific genotypes circulating in the area and a vaccine targeting HPV 16, 18, 45, 52, and 58 may be optimal for the control of cervical cancer in the two countries.
authors.affiliation: Department of Immunology and Molecular Biology, University of Gondar, Gondar, Ethiopia. ebbaabate@yahoo.com
authors.forename: Ebba
authors.initials: E
authors.lastname: Abate
authors.forename: Abraham
authors.initials: A
authors.lastname: Aseffa
authors.forename: Muntasir
authors.initials: M
authors.lastname: El-Tayeb
authors.forename: Ibrahim
authors.initials: I
authors.lastname: El-Hassan
authors.forename: Lawrence
authors.initials: L
authors.lastname: Yamuah
authors.forename: Wude
authors.initials: W
authors.lastname: Mihret
authors.forename: Liku
authors.initials: L
authors.lastname: Bekele
authors.forename: Senait
authors.initials: S
authors.lastname: Ashenafi
authors.forename: Nadia
authors.initials: N
authors.lastname: El-Dawi
authors.forename: Meseret
authors.initials: M
authors.lastname: Belayneh
authors.forename: Ahmed
authors.initials: A
authors.lastname: El-Hassan
authors.forename: Howard
authors.initials: H
authors.lastname: Engers
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D005002:Ethiopia; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000821:virology*; D010232:Paraffin; D016133:Polymerase Chain Reaction; D015995:Prevalence; D013397:Sudan; D016610:Tissue Embedding; D002583:Uterine Cervical Neoplasms / Q000473:pathology* / Q000821:virology*; D055815:Young Adult
chemical_list: D010232:Paraffin