title: Methylation of the WNT5A gene is frequently detected in early gastric carcinoma.
abstract: OBJECTIVE
  Recently, it has been reported that WNT5A methylation was frequently detected in colorectal cancers. However, the relationship between the WNT5A methylation and the characteristics of gastric cancer remains unknown.
  
  
  METHODS
  Methylation status of the WNT5A gene was examined in primary carcinomas and the corresponding normal tissues derived from 38 patients with gastric cancer using quantitative methylation-specific PCR (qMSP) and the correlation between the methylation status and the clinicopathological findings was evaluated.
  
  
  RESULTS
  Aberrant methylation of the WNT5A gene was detected in 7 out of the 38 (18%) primary gastric carcinomas, suggesting that the methylation of WNT5A is observed in gastric carcinomas as well as colorectal ones. The clinicopathological data were correlated with the methylation results. A significant difference was observed in the extent of tumor (p=0.0226). Moreover, a trend was shown towards early TNM stages in methylated tumors (p=0.209).
  
  
  CONCLUSIONS
  WNT5A was more frequently methylated in early gastric carcinomas.
authors.affiliation: Showa University, Yokohama, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Hibi
authors.forename: Makiko
authors.initials: M
authors.lastname: Sakata
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Yokomizi
authors.forename: Yo-hei
authors.initials: YH
authors.lastname: Kitamura
authors.forename: Kazuma
authors.initials: K
authors.lastname: Sakuraba
authors.forename: Rnatsushi
authors.initials: R
authors.lastname: Shirahata
authors.forename: Tetsuhiro
authors.initials: T
authors.lastname: Goto
authors.forename: Hiroki
authors.initials: H
authors.lastname: Mizukami
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Saito
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Ishibashi
authors.forename: Gaku
authors.initials: G
authors.lastname: Kigawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nemoto
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Sanada
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D016009:Chi-Square Distribution; D019175:DNA Methylation; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D051153:Wnt Proteins / Q000235:genetics*; D000071818:Wnt-5a Protein
chemical_list: D011518:Proto-Oncogene Proteins; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein