title: Simulation of two dimensional electrophoresis and tandem mass spectrometry for teaching proteomics.
abstract: In proteomics, complex mixtures of proteins are separated (usually by chromatography or electrophoresis) and identified by mass spectrometry. We have created 2DE Tandem MS, a computer program designed for use in the biochemistry, proteomics, or bioinformatics classroom. It contains two simulations-2D electrophoresis and tandem mass spectrometry. The two simulations are integrated together and are designed to teach the concept of proteome analysis of prokaryotic and eukaryotic organisms. 2DE-Tandem MS can be used as a freestanding simulation, or in conjunction with a wet lab, to introduce proteomics in the undergraduate classroom. 2DE Tandem MS is a free program available on Sourceforge at https://sourceforge.net/projects/jbf/. It was developed using Java Swing and functions in Mac OSX, Windows, and Linux, ensuring that every student sees a consistent and informative graphical user interface no matter the computer platform they choose. Java must be installed on the host computer to run 2DE Tandem MS. Example classroom exercises are provided in the Supporting Information.
authors.affiliation: School of Chemistry & Materials Science, Rochester Institute of Technology, Rochester, NY, USA.
authors.forename: Amanda
authors.initials: A
authors.lastname: Fisher
authors.forename: Emily
authors.initials: E
authors.lastname: Sekera
authors.forename: Jill
authors.initials: J
authors.lastname: Payne
authors.forename: Paul
authors.initials: P
authors.lastname: Craig
mesh_terms: D000818:Animals; D001671:Biochemistry / Q000193:education* / Q000379:methods*; D019295:Computational Biology / Q000193:education*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D020407:Internet; D040901:Proteomics / Q000193:education* / Q000379:methods*; D012984:Software; D053719:Tandem Mass Spectrometry; D013663:Teaching / Q000379:methods