title: Transduced Tat-glyoxalase protein attenuates streptozotocin-induced diabetes in a mouse model.
abstract: Diabetes mellitus (DM) is characterized by hyperglycemia. Glyoxalase 1 (GLO) has considerable potential as a possible therapeutic agent for DM. However, the precise action of GLO remains unclear in DM. In this study, we examined the protective effects of GLO protein in a streptozotocin (STZ)-induced diabetes animal model using cell-permeable Tat-GLO protein. Purified Tat-GLO protein was efficiently transduced into RINm5F cells in a time- and dose-dependent manner and protected cells against sodium nitroprusside (SNP)-induced cell death and DNA fragmentation. Furthermore, Tat-GLO protein significantly inhibited blood glucose levels and altered the serum biochemical parameters in STZ-induced diabetic mice. These results demonstrate that transduced Tat-GLO protein protects pancreatic cells by the inhibition of STZ-mediated toxicity. Therefore, Tat-GLO protein could be useful as a therapeutic agent against DM.
authors.affiliation: Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 200-702, Republic of Korea.
authors.forename: Mi Jin
authors.initials: MJ
authors.lastname: Kim
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Kim
authors.forename: Byung Ryong
authors.initials: BR
authors.lastname: Lee
authors.forename: Min Jea
authors.initials: MJ
authors.lastname: Shin
authors.forename: Young Nam
authors.initials: YN
authors.lastname: Kim
authors.forename: Seon Ae
authors.initials: SA
authors.lastname: Eom
authors.forename: Byung-Jae
authors.initials: BJ
authors.lastname: Park
authors.forename: Yoon Shin
authors.initials: YS
authors.lastname: Cho
authors.forename: Kyu Hyung
authors.initials: KH
authors.lastname: Han
authors.forename: Jinseu
authors.initials: J
authors.lastname: Park
authors.forename: Hyun Sook
authors.initials: HS
authors.lastname: Hwang
authors.forename: Won Sik
authors.initials: WS
authors.lastname: Eum
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choi
mesh_terms: D000818:Animals; D002460:Cell Line; D002463:Cell Membrane Permeability; D003921:Diabetes Mellitus, Experimental / Q000517:prevention & control / Q000628:therapy*; D006801:Humans; D007791:Lactoylglutathione Lyase / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010446:Peptide Fragments / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000008:administration & dosage* / Q000378:metabolism; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism
chemical_list: D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D054322:tat Gene Products, Human Immunodeficiency Virus; D007791:Lactoylglutathione Lyase