title: Upregulation of TRIM5α gene expression after live-attenuated simian immunodeficiency virus vaccination in Mauritian cynomolgus macaques, but TRIM5α genotype has no impact on virus acquisition or vaccination outcome.
abstract: Polymorphism in the TRIM5α/TRIMcyp gene, which interacts with the lentiviral capsid, has been shown to impact on simian immunodeficiency virus (SIV) replication in certain macaque species. Here, in the context of a live-attenuated SIV vaccine study conducted in Mauritian-origin cynomolgus macaques (MCM), we demonstrate upregulation of TRIM5α expression in multiple lymphoid tissues immediately following vaccination. Despite this, the restricted range of TRIM5α genotypes and lack of TRIMcyp variants had no or only limited impact on the replication kinetics in vivo of either the SIVmac viral vaccine or wild-type SIVsmE660 challenge. Additionally, there appeared to be no impact of TRIM5α genotype on the outcome of homologous or heterologous vaccination/challenge studies. The limited spectrum of TRIM5α polymorphism in MCM appears to minimize host bias to provide consistency of replication for SIVmac/SIVsm viruses in vivo, and therefore on vaccination and pathogenesis studies conducted in this species.
authors.affiliation: Divison of Retrovirology, National Institute for Biological Standards and Control-Health Protection Agency, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK.
authors.forename: Giada
authors.initials: G
authors.lastname: Mattiuzzo
authors.affiliation: Divison of Retrovirology, National Institute for Biological Standards and Control-Health Protection Agency, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK.
authors.forename: Nicola J
authors.initials: NJ
authors.lastname: Rose
authors.affiliation: Divison of Retrovirology, National Institute for Biological Standards and Control-Health Protection Agency, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK.
authors.forename: Neil
authors.initials: N
authors.lastname: Almond
authors.affiliation: Division of Infection and Immunity, University College London, Gower Street, London WC1E 6BT, UK.
authors.forename: Greg J
authors.initials: GJ
authors.lastname: Towers
authors.affiliation: Divison of Retrovirology, National Institute for Biological Standards and Control-Health Protection Agency, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK.
authors.forename: Neil
authors.initials: N
authors.lastname: Berry
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D005838:Genotype; D008252:Macaca fascicularis; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics / Q000378:metabolism; D016097:Simian Acquired Immunodeficiency Syndrome / Q000517:prevention & control* / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D013045:Species Specificity; D013997:Time Factors; D014765:Viral Vaccines; D014779:Virus Replication
chemical_list: D002352:Carrier Proteins; D014765:Viral Vaccines; D012313:RNA