title: Efficacy of cocktail phage therapy in treating Vibrio cholerae infection in rabbit model.
abstract: Ability of a cocktail of five lytic vibriophages to combatting Vibrio cholerae O1 infection in rabbit model was examined. In one group, rabbits were administered 1 × 10(8) plaque forming unit of phage cocktail 6 and 12 h prior to the administration of V. cholerae O1, while in the other group, same procedure was applied 6 and 12 h post infection. It was observed that oral administration of the phage cocktail after oral bacterial challenge lowered the shedding of bacteria significantly (p < 0.01). In contrast phage treatment prior to bacterial challenge had no such effect (p > 0.05). Results suggest that oral administration of phage subsequent to V. cholerae challenge could provide a possible means of combatting V. cholerae infection.
authors.affiliation: Division of Bacteriology, National Institute of Cholera and Enteric Diseases, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Jaiswal
authors.forename: Hemanta
authors.initials: H
authors.lastname: Koley
authors.forename: Amit
authors.initials: A
authors.lastname: Ghosh
authors.forename: Anup
authors.initials: A
authors.lastname: Palit
authors.forename: Banwarilal
authors.initials: B
authors.lastname: Sarkar
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001435:Bacteriophages / Q000502:physiology*; D002771:Cholera / Q000382:microbiology / Q000628:therapy*; D015169:Colony Count, Microbial; D005243:Feces / Q000382:microbiology / Q000821:virology; D005260:Female; D008297:Male; D023421:Models, Animal; D011817:Rabbits; D013997:Time Factors; D014734:Vibrio cholerae / Q000821:virology*; D010948:Viral Plaque Assay