title: RNA SHAPE analysis in living cells.
abstract: RNA structure has important roles in practically every facet of gene regulation, but the paucity of in vivo structural probes limits current understanding. Here we design, synthesize and demonstrate two new chemical probes that enable selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) in living cells. RNA structures in human, mouse, fly, yeast and bacterial cells are read out at single-nucleotide resolution, revealing tertiary contacts and RNA-protein interactions.
authors.affiliation: Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Spitale
authors.forename: Pete
authors.initials: P
authors.lastname: Crisalli
authors.forename: Ryan A
authors.initials: RA
authors.lastname: Flynn
authors.forename: Eduardo A
authors.initials: EA
authors.lastname: Torre
authors.forename: Eric T
authors.initials: ET
authors.lastname: Kool
authors.forename: Howard Y
authors.initials: HY
authors.lastname: Chang
mesh_terms: D000215:Acylation; D000818:Animals; D006801:Humans; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*
chemical_list: D012313:RNA