title: Experimental approaches for gene regulatory network construction: the chick as a model system.
abstract: Setting up the body plan during embryonic development requires the coordinated action of many signals and transcriptional regulators in a precise temporal sequence and spatial pattern. The last decades have seen an explosion of information describing the molecular control of many developmental processes. The next challenge is to integrate this information into logic "wiring diagrams" that visualize gene actions and outputs, have predictive power and point to key control nodes. Here, we provide an experimental workflow on how to construct gene regulatory networks using the chick as model system.
authors.affiliation: Department of Craniofacial Development and Stem Cell Biology, King's College London, London, United Kingdom. andrea.streit@kcl.ac.uk
authors.forename: Andrea
authors.initials: A
authors.lastname: Streit
authors.forename: Monica
authors.initials: M
authors.lastname: Tambalo
authors.forename: Jingchen
authors.initials: J
authors.lastname: Chen
authors.forename: Timothy
authors.initials: T
authors.lastname: Grocott
authors.forename: Maryam
authors.initials: M
authors.lastname: Anwar
authors.forename: Alona
authors.initials: A
authors.lastname: Sosinsky
authors.forename: Claudio D
authors.initials: CD
authors.lastname: Stern
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks