title: Simultaneous measurement of SUMOylation using SNAP/CLIP-tag-mediated translation at the single-molecule level.
abstract: Two opposing SUMO wrestlers: simultaneous measurement of multiple SUMOylations was achieved at the single-molecule level by integrating SNAP/CLIP-tag-mediated translation with single-molecule detection (SUMO=small ubiquitin-like modifier). This method gives exceptional sensitivity, and is capable of measuring the SUMOylation of different proteins under various physiological conditions.
authors.affiliation: Single-molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Guangdong, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.forename: Chun-yang
authors.initials: CY
authors.lastname: Zhang
mesh_terms: D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D058207:Sumoylation
chemical_list: D011506:Proteins