title: Study of peripheral BRAF(V600E) mutation as a possible novel marker for papillary thyroid carcinomas.
abstract: BACKGROUND
  The BRAF(V600E) mutation can be detected peripherally in the serum of patients with thyroid cancer. The purpose of this study was to establish the value of detecting the peripheral BRAF(V600E) mutation as a serum tumor marker in this population.
  
  
  METHODS
  In this study, we obtained 94 serum samples from patients with papillary thyroid cancer positive for the BRAF(V600E) mutation in the tumor itself. The serum samples were analyzed for BRAF(V600E) mutation using real-time polymerase chain reaction (PCR).
  
  
  RESULTS
  Sixty-seven patients (71.3%) had papillary thyroid microcarcinoma and 26 patients (27.7%) had underlying lymphocytic thyroiditis. Forty-three patients (45.7%) were found to have stage III or stage IV thyroid cancer. None of the patients had a detectable serum BRAF(V600E) mutation.
  
  
  CONCLUSIONS
  We were unable to identify peripheral BRAF(V600E) mutations in patients with papillary thyroid cancer using real-time PCR. Further studies will be needed to validate our results using various diagnostic methods.
authors.affiliation: Department of Radiology, Research Institute of Radiological Science, Yonsei, University College of Medicine, Seoul, Korea.
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Kwak
authors.forename: Jong Ju
authors.initials: JJ
authors.lastname: Jeong
authors.forename: Sang-Wook
authors.initials: SW
authors.lastname: Kang
authors.forename: Seulkee
authors.initials: S
authors.lastname: Park
authors.forename: Jong Rak
authors.initials: JR
authors.lastname: Choi
authors.forename: Seo-Jin
authors.initials: SJ
authors.lastname: Park
authors.forename: Eun Kyung
authors.initials: EK
authors.lastname: Kim
authors.forename: Woong Youn
authors.initials: WY
authors.lastname: Chung
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics*; D002277:Carcinoma / Q000097:blood / Q000235:genetics* / Q000601:surgery; D002291:Carcinoma, Papillary; D015331:Cohort Studies; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D011379:Prognosis; D011446:Prospective Studies; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000097:blood / Q000235:genetics* / Q000601:surgery; D013965:Thyroidectomy / Q000379:methods; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D048493:Proto-Oncogene Proteins B-raf