title: Mapping of carboxypeptidase m in normal human kidney and renal cell carcinoma: expression in tumor-associated neovasculature and macrophages.
abstract: Although the kidney generally has been regarded as an excellent source of carboxypeptidase M (CPM), little is known about its renal-specific expression level and distribution. This study provides a detailed localization of CPM in healthy and diseased human kidneys. The results indicate a broad distribution of CPM along the renal tubular structures in the healthy kidney. CPM was identified at the parietal epithelium beneath the Bowman's basement membrane and in glomerular mesangial cells. Capillaries, podocytes, and most interstitial cells were CPM negative. Tumor cells of renal cell carcinoma subtypes lose CPM expression upon dedifferentiation. Tissue microarray analysis demonstrated a correlation between low CPM expression and tumor cell type. CPM staining was intense on phagocytotic tumor-associated macrophages. Immunoreactive CPM was also detected in the tumor-associated vasculature. The absence of CPM in normal renal blood vessels points toward a role for CPM in angiogenesis. Coexistence of CPM and the epidermal growth factor receptor (EGFR) was detected in papillary renal cell carcinoma. However, the different subcellular localization of CPM and EGFR argues against an interaction between these h proteins. The description of the distribution of CPM in human kidney forms the foundation for further study of the (patho)physiological activities of CPM in the kidney.
authors.affiliation: Laboratory of Medical Biochemistry, University of Antwerp, Belgium.
authors.forename: Catherine J
authors.initials: CJ
authors.lastname: Denis
authors.forename: Nathalie
authors.initials: N
authors.lastname: Van Acker
authors.forename: Stefanie
authors.initials: S
authors.lastname: De Schepper
authors.forename: Martine
authors.initials: M
authors.lastname: De Bie
authors.forename: Luc
authors.initials: L
authors.lastname: Andries
authors.forename: Erik
authors.initials: E
authors.lastname: Fransen
authors.forename: Dirk
authors.initials: D
authors.lastname: Hendriks
authors.forename: Mark M
authors.initials: MM
authors.lastname: Kockx
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Lambeir
mesh_terms: D000328:Adult; D002292:Carcinoma, Renal Cell / Q000098:blood supply / Q000201:enzymology* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000473:pathology; D066246:ErbB Receptors / Q000032:analysis; D058851:GPI-Linked Proteins / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000098:blood supply / Q000201:enzymology* / Q000473:pathology*; D007680:Kidney Neoplasms / Q000098:blood supply / Q000201:enzymology* / Q000473:pathology; D008264:Macrophages / Q000201:enzymology* / Q000473:pathology; D008666:Metalloendopeptidases / Q000032:analysis*; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000201:enzymology / Q000473:pathology; D046888:Tissue Array Analysis
chemical_list: D058851:GPI-Linked Proteins; D066246:ErbB Receptors; C058479:carboxypeptidase M; D008666:Metalloendopeptidases