title: Evolution of microbial community diversity and enzymatic activity during composting.
abstract: The composting of organic material is dependent on microbial activity. However, the dynamics of the microbial community during the composting process remain obscure. Here, denaturing gradient gel electrophoresis of 16S rDNA amplicons in a chicken manure-based compost was applied to characterize the components of the microbial community during the composting process. In addition, the activity of key microbial enzymes was monitored. Arcobacter spp. and Marinospirillum spp. were the dominant species prior to composting, whereas Thermotogae spp. became more strongly represented as the composting process proceeded. Bacillus and Cohnella spp. were featured at various phases. Correlation analysis showed that the diversity of the microbial community was positively correlated with the compost pH, its total nitrogen level, its carbon-to-nitrogen ratio and the activity of protease, and negatively correlated with its organic carbon content and seed germination indices.
authors.affiliation: South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: He
authors.forename: Kaizhi
authors.initials: K
authors.lastname: Xie
authors.forename: Peizhi
authors.initials: P
authors.lastname: Xu
authors.forename: Xu
authors.initials: X
authors.lastname: Huang
authors.forename: Wenjie
authors.initials: W
authors.lastname: Gu
authors.forename: Fabao
authors.initials: F
authors.lastname: Zhang
authors.forename: Shuanhu
authors.initials: S
authors.lastname: Tang
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D058448:Biota; D002244:Carbon / Q000032:analysis; D002645:Chickens; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D006863:Hydrogen-Ion Concentration; D008372:Manure / Q000382:microbiology; D054892:Metagenome; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000032:analysis; D010447:Peptide Hydrolases / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D008372:Manure; D012336:RNA, Ribosomal, 16S; D012987:Soil; D002244:Carbon; D010447:Peptide Hydrolases; D009584:Nitrogen