title: Hoeflea suaedae sp. nov., an endophytic bacterium isolated from the root of the halophyte Suaeda maritima.
abstract: A Gram-negative, aerobic, short rod-shaped bacterium, designated strain YC6898(T), was isolated from the surface-sterilized root of a halophyte (Suaeda maritima) inhabiting tidal flat of Namhae Island, Korea. Strain YC6898(T) grew optimally at 30-37 °C and pH 6.5-7.5. The strain inhibited mycelial growth of Pythium ultimum and Phytophthora capsici. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YC6898(T) belongs to the genus Hoeflea in the family Phyllobacteriaceae. Its closest relatives were Hoeflea alexandrii AM1V30(T) (96.7% 16S rRNA gene sequence similarity), Hoeflea anabaenae WH2K(T) (95.7%), Hoeflea phototrophica DFL-43(T) (95.5%) and Hoeflea marina LMG 128(T) (94.8%). Strain YC6898(T) contained Q-10 as the major ubiquinone. The major fatty acids of strain YC6898(T) were C18:1ω7c (61.1%), C16:0 (11.9%), 11-methyl C18:1ω7c (9.6%) and C19:0 cyclo ω8c (8.0%). The polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, unknown lipids and an unknown glycolipid. The total genomic DNA G+C content was 53.7 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic analysis, strain YC6898(T) represents a novel species of the genus Hoeflea, for which the name Hoeflea suaedae sp. nov. is proposed. The type strain is YC6898(T) (=KACC 14911(T)=NBRC 107700(T)).
authors.affiliation: Department of Research & Technology Development, JGreen Inc., Jinju 660-841, Republic of Korea.
authors.forename: Eu Jin
authors.initials: EJ
authors.lastname: Chung
authors.affiliation: Division of Applied Life Science (BK21), Plant Molecular Biology & Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Jeong Ae
authors.initials: JA
authors.lastname: Park
authors.affiliation: Division of Applied Life Science (BK21), Plant Molecular Biology & Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Prabhat
authors.initials: P
authors.lastname: Pramanik
authors.affiliation: Division of Applied Life Science (BK21), Plant Molecular Biology & Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Fehmida
authors.initials: F
authors.lastname: Bibi
authors.affiliation: Department of Life Sciences, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Che Ok
authors.initials: CO
authors.lastname: Jeon
authors.affiliation: Division of Applied Life Science (BK21), Plant Molecular Biology & Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Young Ryun
authors.initials: YR
authors.lastname: Chung
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D019663:Chenopodiaceae / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D059127:Phyllobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2