title: Use of metaphase I oocytes matured in vitro is associated with embryo multinucleation.
abstract: OBJECTIVE
  To evaluate the impact of oocyte maturational stage at retrieval on embryo multinucleation.
  
  
  METHODS
  Retrospective study.
  
  
  METHODS
  Private institution for assisted reproduction.
  
  
  METHODS
  A total of 412 patients undergoing 500 intracytoplasmic sperm injection (ICSI) cycles between August 2006 and September 2010.
  
  
  METHODS
  Routine ICSI laboratory procedures.
  
  
  METHODS
  Normal and abnormal fertilization; embryo development; arrest at pronuclear stage; failure to undergo first mitotic division; presence of embryo multinucleation; embryo quality; pregnancy, implantation, and miscarriage rates.
  
  
  RESULTS
  A significantly lower percentage of multinucleation was found in embryos originating from metaphase II (MII) oocytes when compared with MI-II- and MI-derived oocytes. Significantly fewer multinucleated cells per embryo were observed in MII-derived oocytes. Clinical pregnancy and implantation rates were significantly higher when only embryos derived from MII oocytes were transferred.
  
  
  CONCLUSIONS
  Embryo multinucleation rate increases when in vitro-matured (2-5 hours incubation) MI (MI-II) oocytes are used instead of in vivo-matured oocytes in ICSI. Furthermore, all other ICSI outcome parameters are also compromised. The use of donated gametes does not modify these results.
authors.affiliation: Centro Médico Seremas, Buenos Aires, Argentina. sdv@seremas.com
authors.forename: Sabrina
authors.initials: S
authors.lastname: De Vincentiis
authors.forename: Evelyn
authors.initials: E
authors.lastname: De Martino
authors.forename: Mariano G
authors.initials: MG
authors.lastname: Buffone
authors.forename: Santiago
authors.initials: S
authors.lastname: Brugo-Olmedo
mesh_terms: D000022:Abortion, Spontaneous / Q000453:epidemiology; D000328:Adult; D001118:Argentina / Q000453:epidemiology; D002467:Cell Nucleus / Q000473:pathology*; D015897:Comorbidity; D010064:Embryo Implantation; D004622:Embryo, Mammalian / Q000166:cytology*; D047108:Embryonic Development; D004627:Embryonic Induction; D005260:Female; D006801:Humans; D007246:Infertility / Q000453:epidemiology / Q000628:therapy*; D008677:Metaphase; D009865:Oocytes / Q000145:classification / Q000473:pathology* / Q000637:transplantation*; D010062:Ovulation Induction; D011247:Pregnancy; D011256:Pregnancy Outcome; D015995:Prevalence; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic; D016896:Treatment Outcome