title: Temperature- and salinity-decoupled overproduction of hydroxyectoine by Chromohalobacter salexigens.
abstract: Hydroxyectoine overproduction by the natural producer Chromohalobacter salexigens is presented in this study. Genetically engineered strains were constructed that at low salinity coexpressed, in a vector derived from a native plasmid, the ectoine (ectABC) and hydroxyectoine (ectD) genes under the control of the ectA promoter, in a temperature-independent manner. Hydroxyectoine production was further improved by increasing the copies of ectD and using a C. salexigens genetic background unable to synthesize ectoines.
authors.affiliation: Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Seville, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Rodríguez-Moya
authors.forename: Montserrat
authors.initials: M
authors.lastname: Argandoña
authors.forename: Fernando
authors.initials: F
authors.lastname: Iglesias-Guerra
authors.forename: Joaquín J
authors.initials: JJ
authors.lastname: Nieto
authors.forename: Carmen
authors.initials: C
authors.lastname: Vargas
mesh_terms: D000599:Amino Acids, Diamino / Q000378:metabolism*; D055353:Chromohalobacter / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D018628:Gene Dosage; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D010957:Plasmids; D054712:Salinity; D013696:Temperature
chemical_list: D000599:Amino Acids, Diamino; C121557:hydroxyectoine