title: Functional relevance of J-protein family of rice (Oryza sativa).
abstract: Protein folding and disaggregation are crucial processes for survival of cells under unfavorable conditions. A network of molecular chaperones supports these processes. Collaborative action of Hsp70 and Hsp100 proteins is an important component of this network. J-proteins/DnaJ members as co-chaperones assist Hsp70. As against 22 DnaJ sequences noted in yeast, rice genome contains 104 J-genes. Rice J-genes were systematically classified into type A (12 sequences), type B (9 sequences), and type C (83 sequences) classes and a scheme of nomenclature of these proteins is proposed. Transcript expression profiles revealed that J-proteins are possibly involved in basal cellular activities, developmental programs, and in stress. Ydj1 is the most abundant J-protein in yeast. Ydj1 deleted yeast cells are nonviable at 37 °C. Two rice ortholog proteins of yeast Ydj1 protein namely OsDjA4 and OsDjA5 successfully rescued the growth defect in mutant yeast. As Hsp70 and J-proteins work in conjunction, it emerges that rice J-proteins can partner with yeast Hsp70 proteins in functioning. It is thus shown that J-protein machine is highly conserved.
authors.affiliation: Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, 110021, India.
authors.forename: Neelam K
authors.initials: NK
authors.lastname: Sarkar
authors.forename: Upasna
authors.initials: U
authors.lastname: Thapar
authors.forename: Preeti
authors.initials: P
authors.lastname: Kundnani
authors.forename: Priyankar
authors.initials: P
authors.lastname: Panwar
authors.forename: Anil
authors.initials: A
authors.lastname: Grover
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D003198:Computer Simulation; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D005816:Genetic Complementation Test; D018869:Heat-Shock Response / Q000235:genetics; D005810:Multigene Family; D009154:Mutation / Q000235:genetics; D012275:Oryza / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics; D009626:Terminology as Topic
chemical_list: D010940:Plant Proteins