title: Effects of a cross-linked hyaluronic acid based gel on the healing of open wounds in dogs.
abstract: OBJECTIVE
  To compare effects of a cross-linked hyaluronic acid (HA) based gel (CMHA-S) to a standard wound management protocol on the healing of acute, full-thickness wounds in dogs.
  
  
  METHODS
  A prospective, controlled, experimental study.
  
  
  METHODS
  Purpose-bred, adult, female beagles (n = 10).
  
  
  METHODS
  Two 2 × 2 cm wounds were surgically created bilaterally on the trunk of each dog and each side randomized to treatment (CMHA-S) or control (CON) groups. Total and open wound areas were measured with digital image planimetry at 15 time points. From these data, percent contraction and percent epithelialization were calculated. Tissue biopsies were obtained at 6 time points and histologic features were scored.
  
  
  RESULTS
  Total wound area was significantly larger and percent contraction was significantly less in CMHA-S compared to CON wounds at all data points between days 9 and 18. At day 25, and for the remainder of the study, CMHA-S wounds were smaller and contracted more than CON wounds, reaching significance at day 32. Percent epithelialization was significantly less in CMHA-S compared to CON wounds at all data points after day 11. Histologically, fibroblastic cellular infiltration was significantly higher in CMHA-S wounds at day 21.
  
  
  CONCLUSIONS
  CMHA-S wounds healed more slowly than CON wounds. This HA-based gel is not indicated in acute, full-thickness skin wounds in dogs as administered in this study. However, treatment may be beneficial in the mid-to-late repair stage of healing, or if scar minimization is desired. Further studies to evaluate the effects of the CMHA-S gel on canine wounds are indicated.
authors.affiliation: Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Heather S
authors.initials: HS
authors.lastname: Hadley
authors.forename: Bryden J
authors.initials: BJ
authors.lastname: Stanley
authors.forename: Michele C
authors.initials: MC
authors.lastname: Fritz
authors.forename: Joe G
authors.initials: JG
authors.lastname: Hauptman
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Steficek
mesh_terms: D000818:Animals; D001458:Bandages / Q000662:veterinary; D004285:Dogs / Q000293:injuries; D005260:Female; D005782:Gels; D006820:Hyaluronic Acid / Q000008:administration & dosage / Q000627:therapeutic use*; D012867:Skin / Q000293:injuries / Q000473:pathology / Q000503:physiopathology; D013997:Time Factors; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D005782:Gels; D006820:Hyaluronic Acid