title: [Effects of oxymatrine on the expressions of pro-collagen and fibronectin of fibroblasts derived from human hyperplastic scars].
abstract: OBJECTIVE
  To study the effects of oxymatrine (OM) on the expressions of pro-collagen I (PC I), pro-collagen II (PC III), fibronectin (FN), matrix metalloproteinase-1 (MMP-1) mRNA of fibroblasts from keloid (KFb), hyperplastic scar (HFb), and normal skin (NFb), and to compare with hydrocortisone (HC).
  
  
  METHODS
  The primary KFb, HFb and NFb were derived from patients and cultured in vitro using tissue block culture method. The fibroblasts were treated with 500 microg/mL OM, 2 microg/mL HC, or without any medicine (as the control). The mRNA expressions of PC I, PC III, FN, MMP-1 of the fibroblasts were detected using RT-PCR.
  
  
  RESULTS
  Under the normal condition, when compared with NFb, the mRNA expressions of PC I of KFb and HFb increased by 31.7% and 34.2% (both P < 0.05). Besides, the mRNA expression of PC III of KFb increased by 44.9% (P < 0.01). OM down-regulated the mRNA expressions of FN and PC I of HFb by 18.8% and 23.6% respectively (both P < 0.05). HC decreased the mRNA expressions of FN and PC I of HFb by 26.8% and 43.6% respectively (P < 0.05, P < 0.01). Meantime, OM up-regulated the mRNA expression of MMP-1 of KFb by 21.8% (P < 0.05).
  
  
  CONCLUSIONS
  OM suppressed the synthesis of extracellular matrix (ECM) possibly through down-regulating the mRNA expressions of PC I and FN. Compared with HC, OM could promote the degradation of ECM through inducing the MMP-1 mRNA expressions of KFb. Therefore, OM could be potentially used in treatment of hypertrophic scar and keloid.
authors.affiliation: Department of Plastic Surgery, Peking University Third Hospital, Beijing.
authors.forename: Xi
authors.initials: X
authors.lastname: Bian
authors.forename: Jiang-qun
authors.initials: JQ
authors.lastname: Wu
authors.forename: Xing-ju
authors.initials: XJ
authors.lastname: Nie
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D002478:Cells, Cultured; D002921:Cicatrix / Q000378:metabolism / Q000473:pathology; D005109:Extracellular Matrix / Q000378:metabolism / Q000473:pathology; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D007627:Keloid / Q000378:metabolism / Q000473:pathology; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D011347:Procollagen / Q000378:metabolism*; D011807:Quinolizines / Q000494:pharmacology*; D000093842:Matrines
chemical_list: D000470:Alkaloids; D005353:Fibronectins; D020781:Matrix Metalloproteinase 1; D011347:Procollagen; D011807:Quinolizines; C037573:oxymatrine; D000093842:Matrines; C522360:MMP1 protein, human