title: Transduction of pressure signal to electrical signal upon sudden increase in turgor pressure in Chara corallina.
abstract: By taking advantage of large cell size of Chara corallina, we analyzed the membrane depolarization induced by decreased turgor pressure (Shimmen in J Plant Res 124:639-644, 2011). In the present study, the response to increased turgor pressure was analyzed. When internodes were incubated in media containing 200 mM dimethyl sulfoxide, their intracellular osmolality gradually increased and reached a steady level after about 3 h. Upon removal of dimethyl sulfoxide, turgor pressure quickly increased. In response to the increase in turgor pressure, the internodes generated a transient membrane depolarization at its nodal end. The refractory period was very long and it took about 2 h for full recovery after the depolarizing response. Involvement of protein synthesis in recovery from refractoriness was suggested, based on experiments using inhibitors.
authors.affiliation: Department of Life Science, University of Hyogo, Hyogo, Japan. shimmen@sci.u-hyogo.ac.jp
authors.forename: Teruo
authors.initials: T
authors.lastname: Shimmen
authors.forename: Koreaki
authors.initials: K
authors.lastname: Ogata
mesh_terms: D002450:Cell Communication; D048429:Cell Size; D044784:Chara / Q000737:chemistry / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D003593:Cytoplasm / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D009994:Osmolar Concentration; D011312:Pressure
chemical_list: D004121:Dimethyl Sulfoxide