title: Hindlimb suspension does not influence mechanical sensitivity, epidermal thickness, and peripheral nerve density in the glabrous skin of the rat hind paw.
abstract: Our aim was to investigate the influence of microgravity on the sensitivity of the skin to mechanical stimulation, epidermal thickness, peripheral nerve density in the upper dermis, and serum levels of a stress marker in a rat hindlimb suspension (HS) model. Thirty 8-week-old male Wistar rats were randomly divided into 3 groups: HS, n=10; sham HS, n=10; control, n=10. The suspension system was attached to rat tails in both the HS and sham-HS groups, but the hindlimbs were suspended only in the HS group. The HS and sham-HS groups were treated for 4 weeks. In behavioral tests using von-Frey filaments (n=5 in each group), mechanical hypersensitivity developed in the HS and sham HS groups. Serum corticosterone levels increased significantly in the HS and sham HS groups compared to the control group, and no changes in epidermal thickness or peripheral nerve density were observed immediately after the removal of HS (n=5 in each group). These data indicated that the mechanical hypersensitivity observed in the HS group was not caused by microgravity or inactivity, but rather by restraint stress. We suggest that microgravity does not affect skin sensitivity and histology in these animals. Unit of Physical Therapy and Occupational Therapy Sciences, Nagasaki University Graduate School of Biochemical Sciences, Nagasaki-shi, Japan.
authors.affiliation: Department of Rehabilitation, Nagasaki Memorial Hospital, Nagasaki-shi, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: J
authors.initials: J
authors.lastname: Nakano
authors.forename: Y
authors.initials: Y
authors.lastname: Hamaue
authors.forename: Y
authors.initials: Y
authors.lastname: Sekino
authors.forename: J
authors.initials: J
authors.lastname: Sakamoto
authors.forename: H
authors.initials: H
authors.lastname: Kataoka
authors.forename: M
authors.initials: M
authors.lastname: Okita
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D015415:Biomarkers / Q000097:blood; D003345:Corticosterone / Q000097:blood; D004195:Disease Models, Animal; D004817:Epidermis / Q000473:pathology*; D006614:Hindlimb; D019417:Hindlimb Suspension; D008297:Male; D040542:Mechanotransduction, Cellular; D010146:Pain / Q000097:blood / Q000209:etiology / Q000503:physiopathology; D058748:Pain Perception; D017288:Pain Threshold; D010525:Peripheral Nerves / Q000378:metabolism / Q000503:physiopathology*; D011312:Pressure; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time; D012867:Skin / Q000294:innervation* / Q000473:pathology; D013997:Time Factors; D043222:Ubiquitin Thiolesterase / Q000378:metabolism
chemical_list: D015415:Biomarkers; C489034:UCHL1 protein, rat; D043222:Ubiquitin Thiolesterase; D003345:Corticosterone