title: Alternative visualization of SDS-PAGE separated phosphoproteins by alizarin red S-aluminum (III)-appended complex.
abstract: A novel fluorescence detection system using a chemosensor for phosphoprotein in gel electrophoretic analysis has been developed. The system employed the alizarin red S-aluminum (III)-appended complex as a fluorescent staining dye to perform the convenient and selective detection of phosphorylated proteins and total proteins in SDS-PAGE, respectively. Therefore, a full and selective map of proteins can be achieved in the same process without resorting to other compatible detection methods. As low as 62.5 ng of α- (seven or eight phosphates) and β-casein (five phosphates), 125 ng of ovalbumin (two phosphates), and κ-casein (one phosphate) can be detected in approximately 135 min, with the linear responses of rigorous quantitation of changes over a 125-4000 ng range. As a result, alizarin red S-aluminum (III) stain may provide a new choice for selective, economic, and convenient visualization of phosphoproteins.
authors.affiliation: College of Pharmacy & Research Institute of Drug Development, Chonnam National University, Gwangju, South Korea.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Hwang
authors.forename: Wei-Tao
authors.initials: WT
authors.lastname: Cong
authors.forename: Li-Tai
authors.initials: LT
authors.lastname: Jin
authors.forename: Jung-Kap
authors.initials: JK
authors.lastname: Choi
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D017607:Aluminum Compounds / Q000737:chemistry*; D000818:Animals; D000880:Anthraquinones / Q000737:chemistry*; D002417:Cattle; D056831:Coordination Complexes / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D000431:Ethanol / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D010750:Phosphoproteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D011817:Rabbits
chemical_list: D017607:Aluminum Compounds; D000880:Anthraquinones; D056831:Coordination Complexes; D005456:Fluorescent Dyes; D010750:Phosphoproteins; C004468:Alizarin Red S; D000431:Ethanol; D019342:Acetic Acid