title: Smart surface for elution of protein-protein bound particles: nanonewton dielectrophoretic forces using atomic layer deposited oxides.
abstract: By increasing the strength of the negative dielectrophoresis force, we demonstrated a significantly improved electrokinetic actuation and switching microsystem that can be used to elute specifically bound beads from the surface. In this work using atomic layer deposition we deposited a pinhole free nanometer-scale thin film oxide as a protective layer to prevent electrodes from corrosion, when applying high voltages (>20 V(pp)) at the electrodes. Then, by exciting the electrodes at high frequency, we capacitively coupled the electrodes to the buffer in order to avoid electric field degradation and, hence, reduction in dielectrophoresis force due to the presence of the insulating oxide layer. To illustrate the functionality of our system, we demonstrated 100% detachment of anti-IgG and IgG bound beads (which is on the same order of magnitude in strength as typical antibody-antigen interactions) from the surface, upon applying the improved negative dielectrophoresis force. The significantly enhanced switching performance presented in this work shows orders of magnitude of improvement in on-to-off ratio and switching response time, without any need for chemical eluting agents, as compared to the previous work. The promising results from this work vindicates that the functionality of this singleplexed platform can be extended to perform a multiplexed bead-based assay where in a single channel an array of proteins are patterned each targeting a different antigen or protein.
authors.affiliation: Stanford Genome Technology Center, Stanford, California 94304, USA. same@stanford.edu
authors.forename: Sam
authors.initials: S
authors.lastname: Emaminejad
authors.forename: Mehdi
authors.initials: M
authors.lastname: Javanmard
authors.forename: Robert W
authors.initials: RW
authors.lastname: Dutton
authors.forename: Ronald W
authors.initials: RW
authors.lastname: Davis
mesh_terms: D058266:Dielectric Spectroscopy / Q000379:methods*; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism; D010087:Oxides / Q000737:chemistry* / Q000378:metabolism; D054730:Protein Interaction Domains and Motifs / Q000502:physiology
chemical_list: D007074:Immunoglobulin G; D010087:Oxides