title: Early life stress induces renal dysfunction in adult male rats but not female rats.
abstract: Maternal separation (MatSep) is a model of behavioral stress during early life. We reported that MatSep exacerbates ANG II-induced hypertension in adult male rats. The aims of this study were to determine whether exposure to MatSep in female rats sensitizes blood pressure to ANG II infusion similar to male MatSep rats and to elucidate renal mechanisms involved in the response in MatSep rats. Wistar Kyoto (WKY) pups were exposed to MatSep 3 h/day from days 2 to 14, while control rats remained with their mothers. ANG II-induced mean arterial pressure (MAP; telemetry) was enhanced in female MatSep rats compared with control female rats but delayed compared with male MatSep rats. Creatinine clearance (Ccr) was reduced in male MatSep rats compared with control rats at baseline and after ANG II infusion. ANG II infusion significantly increased T cells in the renal cortex and greater histological damage in the interstitial arteries of male MatSep rats compared with control male rats. Plasma testosterone was greater and estradiol was lower in male MatSep rats compared with control rats with ANG II infusion. ANG II infusion failed to increase blood pressure in orchidectomized male MatSep and control rats. Female MatSep and control rats had similar Ccr, histological renal analysis, and sex hormones at baseline and after ANG II infusion. These data indicate that during ANG II-induced hypertension, MatSep sensitizes the renal phenotype in male but not female rats.
authors.affiliation: Section of Experimental Medicine, Georgia Health Sciences Univ., Augusta, GA 30912, USA.
authors.forename: Analia S
authors.initials: AS
authors.lastname: Loria
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Yamamoto
authors.forename: David M
authors.initials: DM
authors.lastname: Pollock
authors.forename: Jennifer S
authors.initials: JS
authors.lastname: Pollock
mesh_terms: D000367:Age Factors; D000375:Aging; D000804:Angiotensin II; D000818:Animals; D001010:Anxiety, Separation / Q000150:complications* / Q000523:psychology; D062186:Arterial Pressure; D018660:Blood Pressure Monitoring, Ambulatory / Q000379:methods; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D004958:Estradiol / Q000097:blood; D005260:Female; D006973:Hypertension / Q000097:blood / Q000139:chemically induced / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology / Q000523:psychology; D007668:Kidney / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D007674:Kidney Diseases / Q000097:blood / Q000139:chemically induced / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology / Q000523:psychology; D008297:Male; D008426:Maternal Deprivation; D009919:Orchiectomy; D011507:Proteinuria / Q000209:etiology / Q000503:physiopathology / Q000523:psychology; D051381:Rats; D011921:Rats, Inbred WKY; D012737:Sex Factors; D013315:Stress, Psychological / Q000150:complications* / Q000523:psychology; D013686:Telemetry; D013739:Testosterone / Q000097:blood
chemical_list: D000804:Angiotensin II; D013739:Testosterone; D004958:Estradiol; D003404:Creatinine