title: Production of pathogen-free horticultural crops by cryotherapy of in vitro-grown shoot tips.
abstract: Horticultural crops are economically valuable for sustainable agricultural production. Plant diseases caused by Pathogens including virus, phytoplasma and bacterium have been a great threat to production of horticultural crops. The efficient use of pathogen-free plant materials has overcome the menace of plant diseases and has sustained crop production. Cryotherapy of shoot tips, a novel application of cryopreservation technique, has become a new plant biotechnology tool for plant pathogen eradication. When compared with the traditional methods, cryotherapy of shoot tips produces high frequency of pathogen-free plants, which is independent of shoot tip size and cryogenic methods. Cryotherapy of shoot tips has six major steps to produce pathogen-free plants: (1) introduction of infected plant materials into in vitro cultures; (2) excision of shoot tips; (3) cryotherapy; (4) post-culture for plant regeneration; (5) indexing of pathogens in regenerated plants after cryotherapy; and (6) establishment of pathogen-free nuclear stock plants. The key steps 2, 3, and 4 are similar to cryopreservation, and play a major role in obtaining high pathogen eradication frequency.
authors.affiliation: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Horticulture, Northwest Agriculture and Forestry University, Shaanxi, China.
authors.forename: Chaohong
authors.initials: C
authors.lastname: Feng
authors.forename: Renrui
authors.initials: R
authors.lastname: Wang
authors.forename: Jingwei
authors.initials: J
authors.lastname: Li
authors.forename: Biao
authors.initials: B
authors.lastname: Wang
authors.forename: Zhenfang
authors.initials: Z
authors.lastname: Yin
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Cui
authors.forename: Baiquan
authors.initials: B
authors.lastname: Li
authors.forename: Wenlu
authors.initials: W
authors.lastname: Bi
authors.forename: Zhibo
authors.initials: Z
authors.lastname: Zhang
authors.forename: Mingfu
authors.initials: M
authors.lastname: Li
authors.forename: Qiaochun
authors.initials: Q
authors.lastname: Wang
mesh_terms: D018556:Crops, Agricultural / Q000254:growth & development* / Q000382:microbiology / Q000502:physiology / Q000821:virology; D003470:Culture Media / Q000737:chemistry; D046508:Culture Techniques / Q000379:methods*; D018520:Plant Shoots / Q000254:growth & development* / Q000382:microbiology / Q000502:physiology / Q000821:virology; D010942:Plant Viruses / Q000502:physiology; D012038:Regeneration; D013696:Temperature
chemical_list: D003470:Culture Media