title: [Effect of gastric bypass surgery on mRNA expression level of hepatic phosphoenolpyruvate carboxykinase in type 2 diabetic Goto-Kakizaki rats].
abstract: OBJECTIVE
  To investigate the effects of gastric bypass surgery(GBP) on hepatic phosphoenolpyruvate carboxykinase(PEPCK) mRNA expression in type 2 diabetic Goto-Kakizaki rats.
  
  
  METHODS
  Male GK rats were randomized into three groups: gastric bypass surgery(n=10), sham operation with diet restriction(n=10), and sham operation alone(n=10). Liver specimens of GK rats were collected during the intraoperative period for self-control study and 8 weeks after surgery. Fasting blood glucose, food intake, and body weight were recorded before surgery and 1, 2, 4, 8 weeks after surgery. The expression of PEPCK mRNA was measured by real-time PCR.
  
  
  RESULTS
  The fasting plasma glucose level decreased from(17.6±2.1) mmol/L before surgery to(7.5±0.9) mmol/L 8 weeks after surgery in GBP group. The level of PEPCK mRNA decreased from 1.08±0.38 before surgery to 0.41±0.10 8 weeks after surgery, significantly lower than that in sham operation alone group(1.04±0.12)(P<0.01). The level of PEPCK mRNA in diet restriction group increased from 1.15±0.16 before surgery to 2.54±0.82 8 weeks after surgery(P<0.01). The expression of PEPCK mRNA in diet restriction was significantly higher than that in CBP group(P<0.01).
  
  
  CONCLUSIONS
  GBP can significantly improve hyperglycemia in type 2 diabetic GK rat models, which may be associated with the decrease of hepatic PEPCK mRNA level.
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital, Wenzhou Medical College, Wenzhou 325027, China.
authors.forename: Jie-bin
authors.initials: JB
authors.lastname: Xie
authors.forename: Jing-li
authors.initials: JL
authors.lastname: Cai
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Li
authors.forename: Lin-jun
authors.initials: LJ
authors.lastname: Zhang
authors.forename: Ming-zhe
authors.initials: MZ
authors.lastname: Jiang
authors.forename: Deng-gang
authors.initials: DG
authors.lastname: Chen
authors.forename: Rong
authors.initials: R
authors.lastname: Chen
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology* / Q000601:surgery; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology* / Q000601:surgery; D015390:Gastric Bypass; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008297:Male; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats
chemical_list: D001786:Blood Glucose; D047908:Intracellular Signaling Peptides and Proteins; D012333:RNA, Messenger; C582554:Pck1 protein, rat; D010729:Phosphoenolpyruvate Carboxykinase (GTP)