title: Chemical characterization, antioxidant and cytotoxic activities of Brazilian red propolis.
abstract: Propolis is known for a long time for its health benefits and biological activities. Here, the red variety from the northeast of Brazil was chemically analyzed and extracts were investigated regarding their antioxidant and antitumor activity. Hydroalcoholic extracts, obtained from the red propolis, revealed polyphenol content, 2,2-diphenyl-1-picrylhydrazyl scavenging potential and enzymatic activities for catalase-like and superoxide dismutase-like. Cytotoxic activity was evaluated for human laryngeal epidermoid carcinoma cell (Hep-2), human cervical adenocarcinoma (HeLa) and human normal epithelial embryonic kidney (Hek-293). Survival analysis for non-tumor cell line showed greater IC50 compared to tumor cell lines, suggesting an increased sensitivity that may correlate with the higher proliferative index of the tumor vs. normal cells. Our results indicate that the Brazilian red propolis is capable of inhibiting cancer cell growth and constitutes an excellent source of antioxidant and antitumor natural agent.
authors.affiliation: Laboratory of Genomics, Proteomics and DNA Repair, University of Caxias do Sul, RS, Brazil.
authors.forename: Caroline Olivieri da Silva
authors.initials: CO
authors.lastname: Frozza
authors.forename: Charlene Silvestrin Celi
authors.initials: CS
authors.lastname: Garcia
authors.forename: Gabriela
authors.initials: G
authors.lastname: Gambato
authors.forename: Marcia Denize Oliveira
authors.initials: MD
authors.lastname: de Souza
authors.forename: Mirian
authors.initials: M
authors.lastname: Salvador
authors.forename: Sidnei
authors.initials: S
authors.lastname: Moura
authors.forename: Francine Ferreira
authors.initials: FF
authors.lastname: Padilha
authors.forename: Fabiana Kömmling
authors.initials: FK
authors.lastname: Seixas
authors.forename: Tiago
authors.initials: T
authors.lastname: Collares
authors.forename: Sibele
authors.initials: S
authors.lastname: Borsuk
authors.forename: Odir Antonio
authors.initials: OA
authors.lastname: Dellagostin
authors.forename: João Antonio Pêgas
authors.initials: JA
authors.lastname: Henriques
authors.forename: Mariana
authors.initials: M
authors.lastname: Roesch-Ely
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D001938:Brazil; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D057809:HEK293 Cells; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D010851:Picrates / Q000378:metabolism; D059808:Polyphenols / Q000032:analysis; D011429:Propolis / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D059808:Polyphenols; D011429:Propolis; C004931:1,1-diphenyl-2-picrylhydrazyl