title: Cadmium and zinc induced similar changes in protein and glycoprotein patterns in tobacco (Nicotiana tabacum l.) seedlings and plants.
abstract: The effects of 10 μmol L-1 and 15 μmol L-1 cadmium (Cd), a nonessential toxic element and 25 μmol L-1 and 50 μmol L-1 zinc (Zn), an essential micronutrient, on proteins and glycoproteins of Nicotiana tabacum L. seedlings and plants were investigated after exposure to each metal alone or to their combinations. Changes in only few polypeptides related to heavy metal treatments were observed in tobacco seedlings and leaves of adult plants, while the greatest change in total soluble protein pattern was observed in plant roots. Differences between control and treated tobacco tissues were more pronounced in the glycoprotein pattern, which was analysed by application of different lectins. The majority of the detected glycoproteins in leaves and roots of adult plants can be considered as a result of enhanced glycosylation due to heavy metal stress. The difference in glycoproteins between Cd and Zn application on tobacco seedlings and adult plants could not be determined since enhanced glycosylation was noticed after treatment with either metal alone or in combination. Therefore, it can be concluded that both metals induced N- and Oglycosylation as a result of changed environmental conditions.
authors.affiliation: Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia.
authors.forename: Petra
authors.initials: P
authors.lastname: Peharec Štefanić
authors.forename: Sandra
authors.initials: S
authors.lastname: Sikić
authors.forename: Petra
authors.initials: P
authors.lastname: Cvjetko
authors.forename: Biljana
authors.initials: B
authors.lastname: Balen
mesh_terms: D002104:Cadmium / Q000493:pharmacokinetics* / Q000633:toxicity*; D045424:Complex Mixtures; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006031:Glycosylation; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000187:drug effects / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism*; D014026:Nicotiana / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D015032:Zinc / Q000493:pharmacokinetics* / Q000494:pharmacology*
chemical_list: D045424:Complex Mixtures; D010940:Plant Proteins; D002104:Cadmium; D015032:Zinc