title: Aggregatibacter actinomycetemcomitans GroEL protein promotes conversion of human CD4+ T cells into IFNγ IL10 producing Tbet+ Th1 cells.
abstract: One of the heat shock family protein (Hsp) expressing bacteria is the gram negative, periodontal pathogen Aggregatibacter actinomycetemcomitans (Aa). A. actinomycetemcomitans' Hsp is a 64-kDa GroEL-protein, which has been shown to influence the host cells. In this study we used recombinant A. actinomycetemcomitans GroEL (rAaGroEL) protein as a model antigen to study GroEL-mediated T cell immune response. Human peripheral mononuclear cells (PBMCs), when stimulated with recombinant rAaGroEL, expressed early activation marker CD69 and IL-2R (CD25). CD25 and CD69 expressions were higher in CD4+ T cells compared to CD8+ T cells. rAaGroEL-responding CD4+ T cells expressed IL-10, IFNγ and TNFα cytokines. Interestingly, there were also IL-10 and IFNγ double cytokine producing CD4+ T cells. Additionally, IFNγ expressing CD4+ T cells were also T-bet positive. Altogether the results suggest that rAaGroEL protein affects CD4+ T cells to differentiate into IFNγ IL10-secreting T-bet+ Th1 cells.
authors.affiliation: Molecular Immunology and Gene Regulation Laboratory, Department of Molecular Biology and Genetics, Izmir Institute of Technology, Urla Izmir, Turkey.
authors.forename: Tahsin
authors.initials: T
authors.lastname: Saygılı
authors.forename: Semih Can
authors.initials: SC
authors.lastname: Akıncılar
authors.forename: Bünyamin
authors.initials: B
authors.lastname: Akgül
authors.forename: Ayten
authors.initials: A
authors.lastname: Nalbant
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000378:metabolism*; D018834:Chaperonin 60 / Q000494:pharmacology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D016753:Interleukin-10 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D011994:Recombinant Proteins / Q000494:pharmacology; D020825:T-Box Domain Proteins / Q000378:metabolism*; D018417:Th1 Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D000098815:T-bet Transcription Factor
chemical_list: D018834:Chaperonin 60; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma