title: Changes of the serum cytokine contents in broilers fed on diets supplemented with nickel chloride.
abstract: Cytokines are immunoregulatory proteins which play an important role in the immune system. The purpose of this study was to examine the serum cytokine contents including interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-6 (IL-6), interleukin-10 (IL-10), interferon gamma (IFN-γ), and tumor necrosis factor-alpha (TNF-α) induced by dietary nickel chloride in broilers by enzyme-linked immunospecific assay. A total of 240 one-day-old avian broilers were divided into four groups and fed on a corn-soybean basal diet as control diet or the same basal diet supplemented with 300, 600, and 900 mg/kg of nickel chloride. During the experimental period of 42 days, the results showed that the serum IL-2, IL-4, IL-6, IL-10, IFN-γ and TNF-α contents were lower (p < 0.05 or p < 0.01) in the 300, 600, 900 mg/kg groups than those in the control group. It was concluded that dietary nickel chloride in the range of 300 to 900 mg/kg could reduce the serum cytokine contents, which could finally impact the immune function in broilers.
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, China.
authors.forename: Bangyuan
authors.initials: B
authors.lastname: Wu
authors.forename: Hengmin
authors.initials: H
authors.lastname: Cui
authors.forename: Xi
authors.initials: X
authors.lastname: Peng
authors.forename: Jing
authors.initials: J
authors.lastname: Fang
authors.forename: Zhicai
authors.initials: Z
authors.lastname: Zuo
authors.forename: Jianying
authors.initials: J
authors.lastname: Huang
authors.forename: Qin
authors.initials: Q
authors.lastname: Luo
authors.forename: Yubing
authors.initials: Y
authors.lastname: Deng
authors.forename: Hesong
authors.initials: H
authors.lastname: Wang
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
mesh_terms: D000821:Animal Feed; D000818:Animals; D002645:Chickens / Q000097:blood / Q000276:immunology* / Q000378:metabolism; D016207:Cytokines / Q000097:blood*; D019587:Dietary Supplements; D007371:Interferon-gamma / Q000097:blood; D016753:Interleukin-10 / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D009532:Nickel / Q000008:administration & dosage* / Q000494:pharmacology; D013025:Glycine max / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D003313:Zea mays / Q000378:metabolism
chemical_list: D016207:Cytokines; D007376:Interleukin-2; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D015847:Interleukin-4; C022838:nickel chloride; D009532:Nickel; D007371:Interferon-gamma