title: [Establishment of a mouse model of ovarian oxidative stress].
abstract: OBJECTIVE
  To evaluate the feasibility of establishing a mouse model of ovarian oxidative stress by intraperitoneal injections of arsenic sodium.
  
  
  METHODS
  Twenty adult female Kunming mice were randomized equally into the normal control group and ovarian oxidative stress model group for intraperitoneal injections of 0.5 ml distilled water and 8 mg/kg arsenic sodium solution every other day, respectively. After 8 injections, the mice were sacrificed for histological observation of the ovarian sections and enzyme-linked immunosorbent assay (ELISA) of serum estradiol (E(2)) and pregnenedione (P) levels ande contents of reactive oxygen species (ROS) , malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the ovary homogenate.
  
  
  RESULTS
  Numerous atretic follicles were found in the ovaries of mice in the model group with obviously reduced growing follicles. Compared with those in the normal control group, the contents of ROS and MDA increased and SOD and GSH-Px levels in the ovarian homogenate decreased significantly in the model group (P<0.05).
  
  
  CONCLUSIONS
  A mouse model of ovarian oxidative stress can be established by intraperitoneal injections of arsenic sodium.
authors.affiliation: Cencer of Reproductive Medicine, Affiliated People's Hospital of Hubei Medical College, Shiyan, China. xnwang2011@163.com
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Wang
authors.forename: Changjun
authors.initials: C
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xue
authors.initials: X
authors.lastname: Ru
authors.forename: Qinqin
authors.initials: Q
authors.lastname: Gong
mesh_terms: D000818:Animals; D018053:Arsenites; D004195:Disease Models, Animal; D005260:Female; D005979:Glutathione Peroxidase / Q000032:analysis; D008315:Malondialdehyde / Q000032:analysis; D051379:Mice; D008815:Mice, Inbred Strains; D010053:Ovary / Q000378:metabolism / Q000503:physiopathology*; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000032:analysis; D013482:Superoxide Dismutase / Q000032:analysis
chemical_list: D018053:Arsenites; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase