title: The rubisco small subunit is involved in tobamovirus movement and Tm-2²-mediated extreme resistance.
abstract: The multifunctional movement protein (MP) of Tomato mosaic tobamovirus (ToMV) is involved in viral cell-to-cell movement, symptom development, and resistance gene recognition. However, it remains to be elucidated how ToMV MP plays such diverse roles in plants. Here, we show that ToMV MP interacts with the Rubisco small subunit (RbCS) of Nicotiana benthamiana in vitro and in vivo. In susceptible N. benthamiana plants, silencing of NbRbCS enabled ToMV to induce necrosis in inoculated leaves, thus enhancing virus local infectivity. However, the development of systemic viral symptoms was delayed. In transgenic N. benthamiana plants harboring Tobacco mosaic virus resistance-2² (Tm-2²), which mediates extreme resistance to ToMV, silencing of NbRbCS compromised Tm-2²-dependent resistance. ToMV was able to establish efficient local infection but was not able to move systemically. These findings suggest that NbRbCS plays a vital role in tobamovirus movement and plant antiviral defenses.
authors.affiliation: Ministry of Education Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Zhao
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.forename: Haili
authors.initials: H
authors.lastname: Zhang
authors.forename: Qi
authors.initials: Q
authors.lastname: Jia
authors.forename: Yiguo
authors.initials: Y
authors.lastname: Hong
authors.forename: Yule
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D015153:Blotting, Western; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism; D060467:Disease Resistance; D020868:Gene Silencing; D054884:Host-Pathogen Interactions; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology; D019441:Plant Epidermis / Q000235:genetics / Q000378:metabolism / Q000821:virology; D018515:Plant Leaves / Q000276:immunology / Q000821:virology; D053830:Plant Viral Movement Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000276:immunology / Q000821:virology; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics / Q000276:immunology / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000276:immunology / Q000378:metabolism* / Q000472:pathogenicity
chemical_list: D053830:Plant Viral Movement Proteins; D012273:Ribulose-Bisphosphate Carboxylase