title: Analysis of p53 and miRNA expression after irradiation of glioblastoma cell lines.
abstract: Glioblastoma is a malignant brain tumor that is difficult to completely cure by surgical treatment alone. However, resistance to anticancer drugs and radiation may be acquired during treatment. For instance, miRNAs involved in regulating the expression of genes inducing apoptosis and other specific genes have been proposed for use, in order to induce the apoptosis of radioresistant cancer cells. A172 glioblastoma cells, expressing wild-type p53 were irradiated to a total dose of up to 60 Gy allowing us to analyze the activities of apoptosis-related proteins. The miR-34a expression levels in cells after irradiation at 30 and 60 Gy were 0.17- and 18.7-times the BCL2 and caspase-9 expression levels, respectively. The high miR-34a expression level in the cells after irradiation at 60 Gy reduced the p53 expression level. This study suggests that apoptosis might be promoted by regulating the action of miRNAs, even in cells that have acquired radioresistance.
authors.affiliation: Department of Neurosurgery, School of Medicine, Showa University, Hatanodai 1-5-8, Shinagawa-ku, Tokyo 142-8666, Japan. sakiko@med.showa-u.ac.jp
authors.forename: Akiko
authors.initials: A
authors.lastname: Sasaki
authors.forename: Yuko
authors.initials: Y
authors.lastname: Udaka
authors.forename: Yuko
authors.initials: Y
authors.lastname: Tsunoda
authors.forename: Gou
authors.initials: G
authors.lastname: Yamamoto
authors.forename: Mayumi
authors.initials: M
authors.lastname: Tsuji
authors.forename: Hideto
authors.initials: H
authors.lastname: Oyamada
authors.forename: Katsuji
authors.initials: K
authors.lastname: Oguchi
authors.forename: Tohru
authors.initials: T
authors.lastname: Mizutani
mesh_terms: D017209:Apoptosis / Q000235:genetics / Q000528:radiation effects; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics / Q000528:radiation effects*; D005909:Glioblastoma / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D011836:Radiation Tolerance / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D035683:MicroRNAs; D016159:Tumor Suppressor Protein p53