title: [A study on the relationship between single nucleotide polymorphisms of interleukin-22 and susceptibility to pulmonary tuberculosis].
abstract: OBJECTIVE
  To study the association between single nucleotide polymorphisms (SNPs) of interleukin-22 (IL-22) gene and pulmonary tuberculosis.
  
  
  METHODS
  From January 2009 to December 2010, clinical data of 479 patients with pulmonary tuberculosis in Shenzhen Third People's Hospital were collected. There were 212 males, and 267 females, aging from 18 to 69 (mean 36 ± 17) years, as well as 358 healthy controls (162 males and 196 females), aging from 18 to 60 (mean 34 ± 13) years. The genotype of SNPs (rs1182844, rs2227473, rs2227476, rs2227480, rs2227485, rs2227508) in IL-22 gene were determined with MassARRAY assay, after Hardy-Weinberg equilibrium test, and the allelic frequency and odds ratio were calculated. Peripheral blood mononuclear cells (PBMCs) from patients with different rs2227473 genotypes were stimulated with anti-CD3 and CD28, and then the IL-22 concentration in supernatant was determined with ELSIA. The SNP allelic frequencies between 2 groups were analyzed by chi-square and IL-22 concentration by t-test.
  
  
  RESULTS
  The frequency of allele G of rs2227473 SNP was significantly higher in tuberculosis group than that in the control group (χ(2) = 7.448, P < 0.01, OR = 1.509, 95%CI= 1.121 - 2.030). The other 5 SNPs allele frequency were not statistically significant between the 2 groups (χ(2) = 0.528 - 3.571, all P > 0.05). The secretion of IL-22 was significantly lower in PBMCs with genotype GG of rs2227473 SNP as compared to that in the others (GA/AA) (t = 2.686, P < 0.01).
  
  
  CONCLUSIONS
  The results suggested that the rs2227473 SNP in IL-22 was associated with the risk of pulmonary tuberculosis. The allele G was the risk factor of pulmonary tuberculosis. The SNP (rs2227473) may play an important role in the protective immune process against tuberculosis by affecting the IL-22 expression of PBMCs.
authors.affiliation: Department of Thoracic Surgery, the First People's Hospital of Shenzhen, Second Affiliated hospital of Jinan University, Shenzhen 518020, China.
authors.forename: Guang-gui
authors.initials: GG
authors.lastname: Ding
authors.forename: Guo-liang
authors.initials: GL
authors.lastname: Zhang
authors.forename: Xin-chun
authors.initials: XC
authors.lastname: Chen
authors.forename: Ming-xia
authors.initials: MX
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007378:Interleukins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D014397:Tuberculosis, Pulmonary / Q000235:genetics*; D055815:Young Adult; D000097043:Interleukin-22
chemical_list: D007378:Interleukins; D000097043:Interleukin-22