title: Identification and characterization of antigenic epitope of Staphylococcus aureus ClfA adhesin.
abstract: Staphylococcus aureus ClfA adhesin is a protective antigen that induces partial immunity against S. aureus infection in mice. To identify the antigenic epitope of ClfA, a monoclonal antibody (mAb) D01 against the recombinant protein was produced by the hybridoma technique. The mAb was used to immunoscreen a random phage-displayed peptide library as the immunogen. After three rounds of biopanning, 41 positive clones were identified. Sixteen phage clones were sequenced and their amino acids were deduced. One mimotope (SKVGIDKRRGTA) showed good match with ClfA adhesin at 383-394 aa and the serum of mice induced by the phage clone clearly recognized ClfA adhesin.
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Ya-hui
authors.initials: YH
authors.lastname: Liu
authors.forename: Zhi-jie
authors.initials: ZJ
authors.lastname: Li
authors.forename: Ya-gang
authors.initials: YG
authors.lastname: Li
authors.forename: Ming-yuan
authors.initials: MY
authors.lastname: Liu
authors.forename: Li-bo
authors.initials: LB
authors.lastname: Liu
authors.forename: Xiao-guang
authors.initials: XG
authors.lastname: Wang
authors.forename: Xue-lin
authors.initials: XL
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Suo
authors.forename: Wen-yu
authors.initials: WY
authors.lastname: Han
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D003030:Coagulase / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D005260:Female; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D019151:Peptide Library; D013203:Staphylococcal Infections / Q000276:immunology; D013211:Staphylococcus aureus / Q000276:immunology*
chemical_list: D018829:Adhesins, Bacterial; D000911:Antibodies, Monoclonal; C515944:ClfA protein, Staphylococcus aureus; D003030:Coagulase; D018985:Epitopes, B-Lymphocyte; D019151:Peptide Library