title: Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration.
abstract: The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen for 12 h. Cryopreserved beads were thawed at 30 degrees C for 1 min, rehydrated using the same liquid mediums [0.75 M sucrose (24 h), 0.5 M sucrose (24 h), 0.25 M sucrose (48 h) and 0.15 M sucrose (24 h)] and cultivated in half strength Murashige & Skoog medium (1962) with the addition of 2 g/L activated charcoal. Sixty four percent of seeds survived and developed into acclimatized plants after being cryopreserved. In this work, the encapsulation-dehydration technique was employed for first time in Cyrtopodium hatschbachii.
authors.affiliation: Instituto de Botánica del Nordeste (IBONE), CONICET--UNNE, Facultad de Ciencias Agrarias (UNNE), C.C. 209, (3400) Corrientes, Argentina. surenciski@yahoo.com
authors.forename: Mauro Rodrigo
authors.initials: MR
authors.lastname: Surenciski
authors.forename: Eduardo Alberto
authors.initials: EA
authors.lastname: Flachsland
authors.forename: Graciela
authors.initials: G
authors.lastname: Terada
authors.forename: Luis Amado
authors.initials: LA
authors.lastname: Mroginski
authors.forename: Hebe Yolanda
authors.initials: HY
authors.lastname: Rey
mesh_terms: D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D003681:Dehydration; D029595:Orchidaceae / Q000187:drug effects / Q000254:growth & development*; D012038:Regeneration; D012639:Seeds / Q000187:drug effects / Q000254:growth & development*
chemical_list: D003451:Cryoprotective Agents