title: Probing the origins of glutathione biosynthesis through biochemical analysis of glutamate-cysteine ligase and glutathione synthetase from a model photosynthetic prokaryote.
abstract: Glutathione biosynthesis catalysed by GCL (glutamate-cysteine ligase) and GS (glutathione synthetase) is essential for maintaining redox homoeostasis and protection against oxidative damage in diverse eukaroytes and bacteria. This biosynthetic pathway probably evolved in cyanobacteria with the advent of oxygenic photosynthesis, but the biochemical characteristics of progenitor GCLs and GSs in these organisms are largely unexplored. In the present study we examined SynGCL and SynGS from Synechocystis sp. PCC 6803 using steady-state kinetics. Although SynGCL shares ~15% sequence identity with the enzyme from plants and α-proteobacteria, sequence comparison suggests that these enzymes share similar active site residues. Biochemically, SynGCL lacks the redox regulation associated with the plant enzymes and functions as a monomeric protein, indicating that evolution of redox regulation occurred later in the green lineage. Site-directed mutagenesis of SynGCL establishes this enzyme as part of the plant-like GCL family and identifies a catalytically essential arginine residue, which is structurally conserved across all forms of GCLs, including those from non-plant eukaryotes and γ-proteobacteria. A reaction mechanism for the synthesis of γ-glutamylcysteine by GCLs is proposed. Biochemical and kinetic analysis of SynGS reveals that this enzyme shares properties with other prokaryotic GSs. Initial velocity and product inhibition studies used to examine the kinetic mechanism of SynGS suggest that it and other prokaryotic GSs uses a random ter-reactant mechanism for the synthesis of glutathione. The present study provides new insight on the molecular mechanisms and evolution of glutathione biosynthesis; a key process required for enhancing bioenergy production in photosynthetic organisms.
authors.affiliation: Department of Biology, Washington University, One Brookings Drive, Campus Box 1137, St. Louis, MO 63130, USA.
authors.forename: William B
authors.initials: WB
authors.lastname: Musgrave
authors.forename: Hankuil
authors.initials: H
authors.lastname: Yi
authors.forename: Dustin
authors.initials: D
authors.lastname: Kline
authors.forename: Jeffrey C
authors.initials: JC
authors.lastname: Cameron
authors.forename: Jonathan
authors.initials: J
authors.lastname: Wignes
authors.forename: Sanghamitra
authors.initials: S
authors.lastname: Dey
authors.forename: Himadri B
authors.initials: HB
authors.lastname: Pakrasi
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Jez
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D005721:Glutamate-Cysteine Ligase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005978:Glutathione / Q000096:biosynthesis / Q000737:chemistry*; D005981:Glutathione Synthase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D009149:Mustard Plant / Q000201:enzymology; D016297:Mutagenesis, Site-Directed; D010788:Photosynthesis; D010940:Plant Proteins / Q000737:chemistry; D016415:Sequence Alignment; D046939:Synechocystis / Q000201:enzymology* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D010940:Plant Proteins; D005721:Glutamate-Cysteine Ligase; D005981:Glutathione Synthase; D005978:Glutathione