title: Interpretation of interlocking key issues of cancer stem cells in malignant solid tumors.
abstract: OBJECTIVE
  In this review, several interlinking issues related to cancer stem cells (CSCs) in malignant solid tumors are sequentially discussed.
  
  
  METHODS
  A literature search was performed using PubMed, Web of Science and the Cochrane library, combining the words CSCs, solid tumor, isolation, identification, origination, therapy, target and epithelial-mesenchymal transition.
  
  
  RESULTS
  Because a primary problem is the isolation of CSCs, we first analyzed the advantages and disadvantages of recently used methods, which were mostly based on the physical or immunochemical characteristics of CSCs. Once CSCs are isolated, they should be identified by their stem cell properties. Here, we suggest how to establish a standard identification strategy. We also focused on the origination hypotheses of CSCs. The supporting molecular mechanisms for each theory were thoroughly analyzed and integrated. Especially, epithelial- mesenchymal transition is an increasingly recognized mechanism to generate CSCs that are endowed with a more invasive and metastatic phenotype. Finally, we discuss putative strategies of eliminating CSCs as effective cancer therapies.
  
  
  CONCLUSIONS
  After several interlocking issues of CSCs are thoroughly clarified, these CSCs in solid malignant tumors may specifically be targeted, which raises a new hope for eliminating these tumors.
authors.affiliation: PLA Center of General Surgery, General Hospital of Chengdu Army Region, Chengdu, Sichuan Province, China.
authors.forename: Wei-Hui
authors.initials: WH
authors.lastname: Liu
authors.forename: Nan
authors.initials: N
authors.lastname: You
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Yan
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Zhu
authors.initials: Z
authors.lastname: Huang
authors.forename: Hong-Bao
authors.initials: HB
authors.lastname: Liu
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Tang
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology / Q000528:radiation effects*; D006801:Humans; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology*; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*