title: Enzymatic amine acyl exchange in peptides on gold surfaces.
abstract: Reversible as well as stereo- and chemoselective: various proteases such as thermolysin and chymotrypsin catalyze amine acyl exchange in peptides. This acyl exchange can be used to modify amino-functionalized surfaces under physiological reaction conditions and provides an alternative mechanism for posttranslational transpeptidation reactions such as peptide-splicing reactions in the proteasome.
authors.affiliation: The School of Chemistry and MIB, The University of Manchester, UK.
authors.forename: Roberto
authors.initials: R
authors.lastname: Castangia
authors.forename: Martina
authors.initials: M
authors.lastname: Austeri
authors.forename: Sabine L
authors.initials: SL
authors.lastname: Flitsch
mesh_terms: D000215:Acylation; D055162:Biocatalysis; D002918:Chymotrypsin / Q000378:metabolism*; D006046:Gold / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013820:Thermolysin / Q000378:metabolism*
chemical_list: D010455:Peptides; D006046:Gold; D002918:Chymotrypsin; D013820:Thermolysin