title: Beneficial role of celery oil in lowering the di(2-ethylhexyl) phthalate-induced testicular damage.
abstract: Di(2-ethylhexyl) phthalate (DEHP), the most abundant phthalate in the environment, is known to be a reproductive toxicant. Considering the therapeutic significance of medicinal plants, this study was conducted to evaluate the effects of administration of celery oil on DEHP-induced testicular toxicity. The experiment was carried out for 8 weeks on 36 male rats that were divided equally into six groups. Group 1 was kept as normal control (given vehicle), while rats of group 2 were administered orally 200 mg/kg/day of celery oil. Groups 3 and 5 were orally given 500 and 1000 mg DEHP/kg/day, respectively. Groups 4 and 6 were treated with similar doses of DEHP as in groups 3 and 5 plus celery oil (200 mg/kg/day). Body and testicular weights, sperm parameters, serum hormones (testosterone, follicle-stimulating hormone, luteinizing hormone and estradiol), antioxidant enzymes (superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT)) and expression of cytochrome P450 aromatase (CYP19) messenger RNA (mRNA) were investigated at the end of 8th week. Treatment with DEHP alone resulted in a significant decrease in body and testicular weights, sperm parameters and serum hormone levels when compared with control. On the other hand, testicular antioxidant enzymes showed a significant dose-dependent increase. The expression of CYP19 mRNA was significantly reduced by increasing the doses of DEHP. Administration of celery oil along with DEHP partially prevented the decrease in body and testicular weights and enhanced epididymal sperm count, serum hormone levels and the expression of CYP19 mRNA along with diminution in the activities of SOD, GPx and CAT enzymes. The obtained results showed that the celery improved the testicular alterations induced by DEHP in albino rats.
authors.affiliation: Department of Zoology, Women College for Arts, Education and Science, Ain Shams University, Cairo, Egypt naglaamadkour@hotmail.com.
authors.forename: Naglaa K
authors.initials: NK
authors.lastname: Madkour
mesh_terms: D000818:Animals; D000077102:Apium / Q000737:chemistry*; D001141:Aromatase / Q000235:genetics / Q000378:metabolism; D001835:Body Weight; D002374:Catalase / Q000378:metabolism; D004051:Diethylhexyl Phthalate / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004822:Epididymis / Q000187:drug effects / Q000378:metabolism; D004958:Estradiol / Q000097:blood; D005640:Follicle Stimulating Hormone / Q000097:blood; D005979:Glutathione Peroxidase / Q000378:metabolism; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012098:Reproduction / Q000187:drug effects; D013076:Sperm Count; D013094:Spermatozoa / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000187:drug effects* / Q000473:pathology; D013739:Testosterone / Q000097:blood
chemical_list: D010938:Plant Oils; D012333:RNA, Messenger; D013739:Testosterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D004051:Diethylhexyl Phthalate; D002374:Catalase; D005979:Glutathione Peroxidase; D001141:Aromatase; D013482:Superoxide Dismutase