title: Plasminogen binding proteins in secreted membrane vesicles of Leishmania mexicana.
abstract: Membrane vesicles secreted by Leishmania mexicana were collected and analyzed. These vesicles can bind plasminogen and were shown to contain enolase, previously identified as a plasminogen-binding protein. In addition, another plasminogen-binding protein was identified, the small myristoylated protein, SMP-1. Recombinant SMP-1 was able to bind plasminogen in a lysine-dependent manner with a K(d) value of 0.24 μM. The C-terminal lysine seems to be responsible for this binding, since this recognition decreases upon carboxypeptidase B treatment. This protein was present within the secreted membrane vesicles as demonstrated by its protection from trypsin digestion in the absence of Triton X-100. Plasminogen-binding proteins in the secreted vesicles may be involved in parasite invasion in the mammalian host.
authors.affiliation: Laboratorio de Fisiología Animal, Departamento de Biología, Universidad de Los Andes, Mérida 5101, Venezuela.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Figuera
authors.forename: Héctor
authors.initials: H
authors.lastname: Acosta
authors.forename: Amaranta
authors.initials: A
authors.lastname: Gómez-Arreaza
authors.forename: Delsy
authors.initials: D
authors.lastname: Dávila-Vera
authors.forename: Alirio
authors.initials: A
authors.lastname: Balza-Quintero
authors.forename: Wilfredo
authors.initials: W
authors.lastname: Quiñones
authors.forename: Rosa Virginia
authors.initials: RV
authors.lastname: Mendoza-Briceño
authors.forename: Juan Luis
authors.initials: JL
authors.lastname: Concepción
authors.forename: Luisana
authors.initials: L
authors.lastname: Avilán
mesh_terms: D054884:Host-Pathogen Interactions; D007700:Kinetics; D007894:Leishmania mexicana / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism; D010958:Plasminogen / Q000378:metabolism*; D011485:Protein Binding; D015800:Protozoan Proteins / Q000378:metabolism*; D022142:Secretory Vesicles / Q000378:metabolism*
chemical_list: D015800:Protozoan Proteins; D010958:Plasminogen; D008239:Lysine