title: Spontaneous reperfusion after in situ thromboembolic stroke in mice.
abstract: Injection of thrombin into the middle cerebral artery (MCA) of mice has been proposed as a new model of thromboembolic stroke. The present study used sequential multiparametric Magnetic Resonance Imaging (MRI), including Magnetic Resonance Angiography (MRA), Diffusion-Weighted Imaging (DWI) and Perfusion-Weighted Imaging (PWI), to document MCA occlusion, PWI-DWI mismatch, and lesion development. In the first experiment, complete MCA occlusion and reproducible hypoperfusion were obtained in 85% of animals during the first hour after stroke onset. In the second experiment, 80% of animals showed partial to complete reperfusion during a three-hour follow-up. Spontaneous reperfusion thus contributed to the variability in ischemic volume in this model. The study confirmed the value of the model for evaluating new thrombolytic treatments, but calls for extended MRI follow-up at the acute stage in therapeutic studies.
authors.affiliation: Université de Lyon, CREATIS, CNRS UMR5220, INSERM U1044, INSA-Lyon, Université Lyon 1, Hospices Civils de Lyon, Lyon, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Durand
authors.forename: Fabien
authors.initials: F
authors.lastname: Chauveau
authors.forename: Tae-Hee
authors.initials: TH
authors.lastname: Cho
authors.forename: Radu
authors.initials: R
authors.lastname: Bolbos
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Langlois
authors.forename: Laure
authors.initials: L
authors.lastname: Hermitte
authors.forename: Marlène
authors.initials: M
authors.lastname: Wiart
authors.forename: Yves
authors.initials: Y
authors.lastname: Berthezène
authors.forename: Norbert
authors.initials: N
authors.lastname: Nighoghossian
mesh_terms: D000818:Animals; D038524:Diffusion Magnetic Resonance Imaging / Q000379:methods; D004195:Disease Models, Animal; D007091:Image Processing, Computer-Assisted; D020244:Infarction, Middle Cerebral Artery / Q000175:diagnosis* / Q000503:physiopathology*; D018810:Magnetic Resonance Angiography / Q000379:methods; D051379:Mice; D015424:Reperfusion; D018709:Statistics, Nonparametric; D013917:Thrombin / Q000008:administration & dosage
chemical_list: D013917:Thrombin