title: The nonfermentable dietary fiber hydroxypropyl methylcellulose modulates intestinal microbiota.
abstract: Diet influences host metabolism and intestinal microbiota; however, detailed understanding of this tripartite interaction is limited. To determine whether the nonfermentable fiber hydroxypropyl methylcellulose (HPMC) could alter the intestinal microbiota and whether such changes correlated with metabolic improvements, C57B/L6 mice were normalized to a high-fat diet (HFD), then either maintained on HFD (control), or switched to HFD supplemented with 10% HPMC, or a low-fat diet (LFD). Compared to control treatment, both LFD and HPMC reduced weight gain (11.8 and 5.7 g, respectively), plasma cholesterol (23.1 and 19.6%), and liver triglycerides (73.1 and 44.6%), and, as revealed by 454-pyrosequencing of the microbial 16S rRNA gene, decreased microbial α-diversity and differentially altered intestinal microbiota. Both LFD and HPMC increased intestinal Erysipelotrichaceae (7.3- and 12.4-fold) and decreased Lachnospiraceae (2.0- and 2.7-fold), while only HPMC increased Peptostreptococcaceae (3.4-fold) and decreased Ruminococcaceae (2.7-fold). Specific microorganisms were directly linked with weight change and metabolic parameters in HPMC and HFD mice, but not in LFD mice, indicating that the intestinal microbiota may play differing roles during the two dietary modulations. This work indicates that HPMC is a potential prebiotic fiber that influences intestinal microbiota and improves host metabolism.
authors.affiliation: Department of Medicine, New York University Langone Medical Center, New York, New York, USA.
authors.forename: Laura M
authors.initials: LM
authors.lastname: Cox
authors.forename: Ilseung
authors.initials: I
authors.lastname: Cho
authors.forename: Scott A
authors.initials: SA
authors.lastname: Young
authors.forename: W H Kerr
authors.initials: WH
authors.lastname: Anderson
authors.forename: Bartholomew J
authors.initials: BJ
authors.lastname: Waters
authors.forename: Shao-Ching
authors.initials: SC
authors.lastname: Hung
authors.forename: Zhan
authors.initials: Z
authors.lastname: Gao
authors.forename: Douglas
authors.initials: D
authors.lastname: Mahana
authors.forename: Monika
authors.initials: M
authors.lastname: Bihan
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Alekseyenko
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Methé
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Blaser
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D044822:Biodiversity; D001835:Body Weight; D018752:Diet, Fat-Restricted; D059305:Diet, High-Fat; D004043:Dietary Fiber / Q000008:administration & dosage*; D065347:Hypromellose Derivatives; D007422:Intestines / Q000382:microbiology*; D055442:Metabolome; D054892:Metagenome / Q000235:genetics; D008747:Methylcellulose / Q000008:administration & dosage / Q000031:analogs & derivatives*; D051379:Mice; D008810:Mice, Inbred C57BL; D010802:Phylogeny; D056692:Prebiotics; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004043:Dietary Fiber; D056692:Prebiotics; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D065347:Hypromellose Derivatives; D008747:Methylcellulose