title: SHBG in GDM maternal serum, placental tissues and umbilical cord serum expression changes and its significance.
abstract: OBJECTIVE
  To compare the expression of sex hormone binding globulin (SHBG) in normal placental tissues and serum with placental tissues from patients with gestational diabetes mellitus (GDM) and to deduce the mechanism affecting placental SHBG in GDM.
  
  
  METHODS
  Enzyme-linked immunosorbent assay (ELISA) method were used to detect SHBG levels changes in normal and GDM maternal serum, umbilical cord serum and placental. We measured SHBG mRNA and protein using reverse-transcription polymerase chain reaction, and Western blotting, respectively, in normal and GDM placental tissues.
  
  
  RESULTS
  In maternal serum, compared with the control group, GDM group: glucose, insulin increased, SHBG decreased. In the placenta, compared with the control group, GDM group: SHBG, SHBG mRNA and SHBG protein decreased. There is no correlation between placenta SHBG and maternal serum SHBG respectively in control and GDM group, but in GDM group maternal serum insulin and placenta SHBG are linear correlation.
  
  
  CONCLUSIONS
  GDM serum and placental SHBG levels are reduced, hyperinsulinemia may lead to a reduction of SHBG in circulating blood, but also damage the placenta cells which led to placental synthesis and secretion of SHBG decrease.
authors.affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital Affiliated to China Medical University, No. 36, Sanhao Street, Heping District, Shenyang, 110004, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.forename: Zhen
authors.initials: Z
authors.lastname: Jin
authors.forename: Weiping
authors.initials: W
authors.lastname: Teng
authors.forename: Xinshu
authors.initials: X
authors.lastname: Chi
authors.forename: Yanan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wanting
authors.initials: W
authors.lastname: Ai
authors.forename: Pinting
authors.initials: P
authors.lastname: Wang
mesh_terms: D000328:Adult; D015153:Blotting, Western; D016640:Diabetes, Gestational / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012738:Sex Hormone-Binding Globulin / Q000235:genetics / Q000378:metabolism*; D014470:Umbilical Cord / Q000378:metabolism*
chemical_list: D012738:Sex Hormone-Binding Globulin