title: Tanshinone II--a inhibits angiogenesis through down regulation of COX-2 in human colorectal cancer.
abstract: Angiogenesis plays a significant role in colorectal cancer (CRC) and cyclooxygenase-2 (COX-2) appears to be involved with multiple aspects of CRC angiogenesis. Our aim was to investigate the inhibitory effects of Tan II-A (Tanshinone II-A, Tan II-A) on tumor growth in mice, as well as alteration of expression of COX-2 and VEGF in CRC. We established the mice xenograft model of C26 CRC cell line, and injected 0.5, 1, 2mg/kg of Tan II-A and 1mg/kg of 5-FU in respectively in vivo. Then, we assayed tumor weight and volume, and evaluated microvascular density and expression of VEGF. COX-2 promoter and COX-2 plasmids were transfected into HCT-116 cells, followed by detection of COX-2 promoter activity by chemiluminescence, and detection of COX-2 mRNA expression by fluorescence quantitative PCR. Taken together, the results showed Tan II-A could inhibit tumor growth and suppress the VEGF level in vivo. HCT-116 cell experiments showed marked inhibitory effects of Tan II-A on COX-2 and VEGF in a dose-dependent manner. The results indicate that Tan II-A can effectively inhibit tumor growth and angiogenesis of human colorectal cancer via inhibiting the expression level of COX-2 and VEGF.
authors.affiliation: Clinic Oncology, Putuo Hospital and Cancer Institute, Shanghai University of Traditional Chinese Medicine, China. Lzwf@hotmail.com
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Zhou
authors.forename: Qiang
authors.initials: Q
authors.lastname: Hu
authors.forename: Hua
authors.initials: H
authors.lastname: Sui
authors.forename: Shu-Jun
authors.initials: SJ
authors.lastname: Ci
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Xuan
authors.initials: X
authors.lastname: Liu
authors.forename: Ning-Ning
authors.initials: NN
authors.lastname: Liu
authors.forename: Pei-Hao
authors.initials: PH
authors.lastname: Yin
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Qin
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
mesh_terms: D045784:Abietanes / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology* / Q000627:therapeutic use; D015179:Colorectal Neoplasms / Q000098:blood supply* / Q000188:drug therapy / Q000378:metabolism*; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism*; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D045325:HCT116 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000098:blood supply* / Q000188:drug therapy / Q000378:metabolism*; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D018709:Statistics, Nonparametric; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors / Q000235:genetics
chemical_list: D045784:Abietanes; D000972:Antineoplastic Agents, Phytogenic; D052246:Cyclooxygenase 2 Inhibitors; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; C021751:tanshinone; D051546:Cyclooxygenase 2