title: 5'-Terminal chemical capping of spliced leader RNAs.
abstract: Spliced leader (SL) RNA trans-splicing adds a 2,2,7-trimethylguanosine cap (TMG) and a 22-nucleotide sequence, the SL, to the 5' end of mRNAs. Both non-trans-spliced with a monomethylguanosine cap (MMG) and trans-spliced mRNAs co-exist in trans-splicing metazoan cells. Efficient translation of TMG-capped mRNAs in nematodes requires a defined core of nucleotides within the SL sequence. Here we present a chemical procedure for the preparation and purification of 5'-terminal capped MMG and TMG wild-type, and mutant 22 nt spliced leader RNAs (GGU/ACUUAAUUACCCAAGUUUGAG) with or without a 3' biotin tag.
authors.affiliation: Faculty of Chemistry, University of Warsaw, 02-093, Warsaw, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Piecyk
authors.forename: Richard E
authors.initials: RE
authors.lastname: Davis
authors.forename: Marzena
authors.initials: M
authors.lastname: Jankowska-Anyszka