title: [The attenuation of myocardial susceptibility to ischemia/reperfusion injury by ischemic postconditioning in hypercholesteremia rats and the role of hypoxia inducible factor-1alpha].
abstract: OBJECTIVE
  To explore whether ischemic postconditioning can attenuate the myocardial injury induced by ischemia/reperfusion (I/R) in hypercholesteremic rats and whether hypoxia inducible factor-1alpha (HIF-1alpha) play a role in the protection.
  
  
  METHODS
  Adult male Wistar rats received a high fat diet for 8 weeks to prepare the hypercholesteremic models. Myocardial damage induced by ischemia/reperfusion was evaluated by infarct size, creatine kinase (CK) activity and myocardial apoptosis. HIF-1alpha mRNA level was detected by real time-RT-PCR and the protein level was detected by Western blot.
  
  
  RESULTS
  Myocardial infarct size, CK activity, and caspase-3 activity induced by I/R were markedly increased in hypercholesteremic rats compared with those in normal rats. Ischemic postconditioning attenuated the myocardial injury in both normal rats and hypercholesteremic rats, and increased HIF-1alpha protein level. There was a significant linear inverse relationship between HIF-1alpha protein level and infarct size (r = -0.802, P <0.01).
  
  
  CONCLUSIONS
  Hypercholesteremia enhanced the susceptibility of myocardia to ischemia/reperfusion injury. While ischemic postconditioning markedly attenuated the increase of myocardial susceptibility to I/R induced by hypercholesteremia. HIF-1alpha might be one of the mechanisms of protection by ischemic postconditioning.
authors.affiliation: Shanxi College of Traditional Chinese Medicine, Taiyuan 030024; 2. Shanxi Medical University, Taiyuan 030001, China. zhhx5@yahoo.com.cn
authors.forename: Huan-Xin
authors.initials: HX
authors.lastname: Zhao
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Li
authors.forename: Ye
authors.initials: Y
authors.lastname: Wu
authors.forename: Xiao-Liang
authors.initials: XL
authors.lastname: Wang
authors.forename: Rong-Rui
authors.initials: RR
authors.lastname: Zhao
authors.forename: Hui-Rong
authors.initials: HR
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D003402:Creatine Kinase / Q000378:metabolism; D004198:Disease Susceptibility; D006937:Hypercholesterolemia / Q000378:metabolism; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D057775:Ischemic Postconditioning; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar
chemical_list: C497444:Hif1a protein, rat; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D003402:Creatine Kinase; C505410:Casp3 protein, rat; D053148:Caspase 3