title: CDC48 function during TMV infection: regulation of virus movement and replication by degradation?
abstract: Cell-division-cycle protein 48 (CDC48) is an essential, conserved ATP-driven chaperone in eukaryotic cells, which functions in diverse cellular processes including the targeting of misfolded and aggregated proteins for degradation via proteasomal and aggresomal-autophagic pathways. We recently demonstrated that plant CDC48 localizes to and interacts with Tobacco mosaic virus (TMV) movement protein (MP) in ER-associated viral protein inclusions. Our data suggest that CDC48 participates in the clearance of these viral protein inclusions in an ER-assisted protein degradation (ERAD)-like mechanism. As TMV MP-inclusions formed at late infection stages resemble aggresomes, we here propose that TMV MP enters both, ERAD-like and aggresomal pathways in its host cells and that CDC48 coordinates these processes. Moreover, as viruses often exploit host pathways for replication and spread, we propose a model in which CDC48 functions in the degradation pathway of overaccumulating viral protein and also actively participates in the regulation of TMV replication and cell-to-cell movement.
authors.affiliation: Botany; Department of Environmental Sciences; University of Basel; Basel, Switzerland.
authors.forename: Annette
authors.initials: A
authors.lastname: Niehl
authors.affiliation: Botany; Department of Environmental Sciences; University of Basel; Basel, Switzerland.
authors.forename: Khalid
authors.initials: K
authors.lastname: Amari
authors.affiliation: Botany; Department of Environmental Sciences; University of Basel; Basel, Switzerland; Institut de Biologie Moléculaire des Plantes; UPR 2357 CNRS; Université de Strasbourg; Strasbourg, France.
authors.forename: Manfred
authors.initials: M
authors.lastname: Heinlein
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D014027:Tobacco Mosaic Virus / Q000502:physiology*; D000074405:Valosin Containing Protein; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication / Q000502:physiology*
chemical_list: D018797:Cell Cycle Proteins; D010940:Plant Proteins; D014764:Viral Proteins; D000251:Adenosine Triphosphatases; D000074405:Valosin Containing Protein