title: Betula pendula Roth leaves: gastroprotective effects of an HPLC-fingerprinted methanolic extract.
abstract: In this study, a methanolic extract of Betula pendula leaves (BLE) was investigated for its gastroprotective effects against 90% ethanol-induced ulcer in rats. Oral pretreatment of rats with BLE (100, 200 and 400 mg kg(- 1)) significantly reduced the incidence of gastric lesions induced by ethanol administration as compared with misoprostol (0.50 mg kg(- 1)). Furthermore, BLE inhibited the increase in malondialdehyde (MDA) and prevented depletion of total sulhydryl and non-protein sulhydryl groups in rat stomach homogenate when compared with ethanol group. With regard to the effect of lipid peroxidation in vitro, BLE showed the ability to reduce methyl linoleate autoxidation. Chemical characterisation of the main biologically active constituents of BLE was also achieved by means of high-performance liquid chromatography with photodiode array and mass spectrometry detection, showing the presence of myricetin-3-O-galactoside, quercetin glycosides, kaempferol glycosides.
authors.affiliation: Dipartimento Farmaco-biologico , University of Messina, Viale Annunziata, 98168, Messina, Italy. germanom@unime.it
authors.forename: Maria Paola
authors.initials: MP
authors.lastname: Germanò
authors.forename: Francesco
authors.initials: F
authors.lastname: Cacciola
authors.forename: Paola
authors.initials: P
authors.lastname: Donato
authors.forename: Paola
authors.initials: P
authors.lastname: Dugo
authors.forename: Giovanna
authors.initials: G
authors.lastname: Certo
authors.forename: Valeria
authors.initials: V
authors.lastname: D'Angelo
authors.forename: Luigi
authors.initials: L
authors.lastname: Mondello
authors.forename: Antonio
authors.initials: A
authors.lastname: Rapisarda
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000737:chemistry / Q000627:therapeutic use; D029662:Betula / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005697:Galactosides / Q000737:chemistry / Q000627:therapeutic use; D005753:Gastric Mucosa / Q000187:drug effects; D044949:Kaempferols / Q000737:chemistry / Q000627:therapeutic use; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D000432:Methanol / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D013276:Stomach Ulcer / Q000139:chemically induced / Q000517:prevention & control
chemical_list: D000897:Anti-Ulcer Agents; D005697:Galactosides; D044949:Kaempferols; D010936:Plant Extracts; C529904:myricetin-3-O-galactoside; D008315:Malondialdehyde; C006552:kaempferol; D000432:Methanol