title: Transcription regulation of a yeast gene from a downstream location.
abstract: Mechanisms for coregulation of transcription of tandem genes in yeast remain largely speculative. This study focused on inositol-mediated regulation of the tandem gene pair SNA3-INO1. While the pattern of regulation of these two genes was similar, results showed that intermediate levels of inositol repressed INO1 and induced SNA3. Results also showed that inositol-mediated regulation of the SNA3 gene was not a function of its promoter but occurred from factors within the SNA3-INO1 intergenic region. The basic helix-loop-helix proteins, Ino2p and Ino4p, mediated this regulation through the upstream activation sequence (UAS)(INO) (E-box) sequences in the intergenic region. These results provide a model for studying coregulation of yeast tandem genes. This is especially significant given that many tandem gene pairs in yeast are coregulated even though context-specific UAS sequences are known only for one gene in the pair.
authors.affiliation: Department of Microbiology, and Molecular Cellular Biology Graduate Program, University of Massachusetts, Amherst, MA 01003, USA.
authors.forename: Ameet
authors.initials: A
authors.lastname: Shetty
authors.forename: Aishwarya
authors.initials: A
authors.lastname: Swaminathan
authors.forename: John M
authors.initials: JM
authors.lastname: Lopes
mesh_terms: D015966:Gene Expression Regulation, Fungal; D007294:Inositol / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D007296:Myo-Inositol-1-Phosphate Synthase / Q000096:biosynthesis* / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; C437323:Sna3 protein, S cerevisiae; D007294:Inositol; C519641:INO1 protein, S cerevisiae; D007296:Myo-Inositol-1-Phosphate Synthase