title: Development of a proteoliposome model to probe transmembrane electron-transfer reactions.
abstract: The mineral-respiring bacterium Shewanella oneidensis uses a protein complex, MtrCAB, composed of two decahaem cytochromes brought together inside a transmembrane porin to transport electrons across the outer membrane to a variety of mineral-based electron acceptors. A proteoliposome system has been developed that contains Methyl Viologen as an internalized electron carrier and valinomycin as a membrane-associated cation exchanger. These proteoliposomes can be used as a model system to investigate MtrCAB function.
authors.affiliation: Centre for Molecular and Structural Biochemistry, School of Biological Sciences and School of Chemistry, University of East Anglia, Norwich NR4 7TJ, UK.
authors.forename: Gaye F
authors.initials: GF
authors.lastname: White
authors.forename: Zhi
authors.initials: Z
authors.lastname: Shi
authors.forename: Liang
authors.initials: L
authors.lastname: Shi
authors.forename: Alice C
authors.initials: AC
authors.lastname: Dohnalkova
authors.forename: James K
authors.initials: JK
authors.lastname: Fredrickson
authors.forename: John M
authors.initials: JM
authors.lastname: Zachara
authors.forename: Julea N
authors.initials: JN
authors.lastname: Butt
authors.forename: David J
authors.initials: DJ
authors.lastname: Richardson
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Clarke
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism* / Q000502:physiology; D004579:Electron Transport; D008081:Liposomes / Q000737:chemistry*; D008954:Models, Biological; D010084:Oxidation-Reduction; D010269:Paraquat / Q000737:chemistry; D020592:Shewanella / Q000378:metabolism*; D014634:Valinomycin
chemical_list: D001425:Bacterial Outer Membrane Proteins; D008081:Liposomes; D014634:Valinomycin; D010269:Paraquat