title: Impact of peptide micropatterning on endothelial cell actin remodeling for cell alignment under shear stress.
abstract: HSVEC behavior under physiological shear stress in vitro is investigated on PET surfaces micropatterned with both RGDS and WQPPRARI peptides. This technique allows (i) creating geometries on surface to guide cell orientation under shear stress and (ii) controlling surface chemical composition in order to modulate cell behavior. Under shear stress, endothelial cells adhere on patterned PET surfaces and present a more rapid orientation in flow direction in comparison to cells cultured on homogeneous surfaces. Micropatterned surfaces presenting a large surface area ratio of RGDS/WQPPRARI peptides induce fibrillar adhesion, while surfaces presenting an equal RGDS/WQPPRARI peptides surface area ratio preferentially induce focal adhesion.
authors.affiliation: Bioingénierie Tissulaire, Université Bordeaux, U1026, F-33000 Bordeaux, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Chollet
authors.forename: Reine
authors.initials: R
authors.lastname: Bareille
authors.forename: Murielle
authors.initials: M
authors.lastname: Rémy
authors.forename: Alain
authors.initials: A
authors.lastname: Guignandon
authors.forename: Laurence
authors.initials: L
authors.lastname: Bordenave
authors.forename: Gaetan
authors.initials: G
authors.lastname: Laroche
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Durrieu
mesh_terms: D000199:Actins / Q000502:physiology*; D000704:Analysis of Variance; D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion / Q000502:physiology*; D042783:Endothelial Cells / Q000502:physiology*; D005353:Fibronectins; D006801:Humans; D066298:In Vitro Techniques; D008856:Microscopy, Fluorescence; D009842:Oligopeptides; D011092:Polyethylene Glycols / Q000737:chemistry*; D011093:Polyethylene Terephthalates; D012501:Saphenous Vein / Q000166:cytology; D033081:Shear Strength / Q000502:physiology*; D013499:Surface Properties; D014654:Vascular Patency / Q000502:physiology*
chemical_list: D000199:Actins; D005353:Fibronectins; D009842:Oligopeptides; D011093:Polyethylene Terephthalates; C417707:fibronectin peptide V; D011092:Polyethylene Glycols; C046617:arginyl-glycyl-aspartyl-serine