title: Impact of ionizing radiation on physicochemical and biological properties of an amphiphilic macromolecule.
abstract: An amphiphilic macromolecule (AM) was exposed to ionizing radiation (both electron beam and gamma) at doses of 25 kGy and 50 kGy to study the impact of these sterilization methods on the physicochemical properties and bioactivity of the AM. Proton nuclear magnetic resonance and gel permeation chromatography were used to determine the chemical structure and molecular weight, respectively. Size and zeta potential of the micelles formed from AMs in aqueous media were evaluated by dynamic light scattering. Bioactivity of irradiated AMs was evaluated by measuring inhibition of oxidized low-density lipoprotein uptake in macrophages. From these studies, no significant changes in the physicochemical properties or bioactivity were observed after the irradiation, demonstrating that the AMs can withstand typical radiation doses used to sterilize materials.
authors.affiliation: Rutgers, The State University of New Jersey, Department of Chemistry and Chemical Biology, 610 Taylor Road, Piscataway, New Jersey 08854, United States.
authors.forename: Li
authors.initials: L
authors.lastname: Gu
authors.forename: Kyle
authors.initials: K
authors.lastname: Zablocki
authors.forename: Linda
authors.initials: L
authors.lastname: Lavelle
authors.forename: Stanko
authors.initials: S
authors.lastname: Bodnar
authors.forename: Frederick
authors.initials: F
authors.lastname: Halperin
authors.forename: Ike
authors.initials: I
authors.lastname: Harper
authors.forename: Prabhas V
authors.initials: PV
authors.lastname: Moghe
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Uhrich