title: Detection of heterogeneity of Borrelia burgdorferi in ixodes ticks by culture-dependent and culture-independent methods.
abstract: Abilities to detect heterogeneity of ospC genotypes of the Lyme disease spirochete Borrelia burgdorferi in the tick vector by in vitro culture (IVC) and direct PCR (dPCR) were compared. IVC failed to detect one-third of the ospC genotypes detected by dPCR. Among IVC results, common ospC genotypes were overrepresented while occurrence of rare genotypes was underestimated.
authors.affiliation: Laboratory of Medical Zoology, Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA.
authors.forename: Guang
authors.initials: G
authors.lastname: Xu
authors.forename: Jaime
authors.initials: J
authors.lastname: Wesker
authors.forename: Caitlin
authors.initials: C
authors.lastname: White
authors.forename: Jason
authors.initials: J
authors.lastname: Campbell
authors.forename: Nicholas G
authors.initials: NG
authors.lastname: Reich
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Rich
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D025065:Borrelia burgdorferi / Q000145:classification / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D018884:Ixodes / Q000382:microbiology*; D008297:Male
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; C079616:OspC protein