title: Simultaneously sensitive detection of multiple miRNAs based on a strand displacement amplification.
abstract: We reported an efficient strategy based on a strand displacement amplification for serum miRNA detection. In such a system, a multiplexed, sensitive and quick detection of miRNAs could be achieved through a combination of fluorescence labeled probes, a common primer and a polymerase. This could be potentially used in the clinical field to achieve early disease diagnosis and prognosis.
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei, 430072, P. R. China.
authors.forename: Tian
authors.initials: T
authors.lastname: Tian
authors.forename: Heng
authors.initials: H
authors.lastname: Xiao
authors.forename: Xiaolian
authors.initials: X
authors.lastname: Zhang
authors.forename: Shuang
authors.initials: S
authors.lastname: Peng
authors.forename: Xiaoe
authors.initials: X
authors.lastname: Zhang
authors.forename: Shan
authors.initials: S
authors.lastname: Guo
authors.forename: Shaoru
authors.initials: S
authors.lastname: Wang
authors.forename: Songmei
authors.initials: S
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.forename: Craig
authors.initials: C
authors.lastname: Meyers
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D035683:MicroRNAs / Q000097:blood*; D021141:Nucleic Acid Amplification Techniques; D012313:RNA / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D035683:MicroRNAs; C034477:RNA primers; D012313:RNA; D012321:DNA-Directed RNA Polymerases