title: New models of hepatitis E virus replication in human and porcine hepatocyte cell lines.
abstract: Hepatitis E virus (HEV) causes acute, enterically transmitted hepatitis in human. It is associated with large epidemics in tropical and subtropical regions where it is endemic or with sporadic cases in non-endemic regions. Unlike other hepatitis viruses, HEV has several animal reservoirs. Phylogenetic studies on HEV human and animal sequences, and the identification of cases of direct transmission from animal to human strongly suggest that HEV is a zoonotic agent. The lack of efficient cell culture models limits studies on molecular and cellular aspects of HEV infection and species barrier crossing. The present study reports on the development of two new in vitro models of HEV replication using a human hepatoma-derived cell line, HepaRG, and a porcine embryonic stem cell-derived cell line, PICM-19. These two cell lines have morphological and functional properties similar to primary hepatocytes. These in vitro culture systems support HEV replication and release of encapsidated RNA. These new models represent a powerful tool for studying the viral replication cycle, species barrier crossing and virulence factors.
authors.affiliation: UMR 1161 Virology, Ecole Nationale Vétérinaire d'Alfort, 94706 Maisons-Alfort, France.|UMR 1161 Virology, INRA, 94706 Maisons-Alfort, France.|UMR 1161 Virology, ANSES, Laboratoire de Santé Animale, 94706 Maisons-Alfort, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Rogée
authors.affiliation: USDA, ARS, Animal and Natural Resources Institute, Beltsville Agricultural Research Center, Beltsville, MD 20705, USA.
authors.forename: Neil
authors.initials: N
authors.lastname: Talbot
authors.affiliation: USDA, ARS, Animal and Natural Resources Institute, Beltsville Agricultural Research Center, Beltsville, MD 20705, USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Caperna
authors.affiliation: UMR 1161 Virology, Ecole Nationale Vétérinaire d'Alfort, 94706 Maisons-Alfort, France.|UMR 1161 Virology, INRA, 94706 Maisons-Alfort, France.|UMR 1161 Virology, ANSES, Laboratoire de Santé Animale, 94706 Maisons-Alfort, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Bouquet
authors.affiliation: UMR 1161 Virology, Ecole Nationale Vétérinaire d'Alfort, 94706 Maisons-Alfort, France.|UMR 1161 Virology, INRA, 94706 Maisons-Alfort, France.|UMR 1161 Virology, ANSES, Laboratoire de Santé Animale, 94706 Maisons-Alfort, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Barnaud
authors.affiliation: UMR 1161 Virology, Ecole Nationale Vétérinaire d'Alfort, 94706 Maisons-Alfort, France.|UMR 1161 Virology, INRA, 94706 Maisons-Alfort, France.|UMR 1161 Virology, ANSES, Laboratoire de Santé Animale, 94706 Maisons-Alfort, France.
authors.forename: Nicole
authors.initials: N
authors.lastname: Pavio
mesh_terms: D000818:Animals; D000914:Antibodies, Viral; D002454:Cell Differentiation; D002460:Cell Line; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D016752:Hepatitis E virus / Q000502:physiology*; D022781:Hepatocytes / Q000821:virology*; D006801:Humans; D012367:RNA, Viral; D013552:Swine; D014779:Virus Replication / Q000502:physiology*
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral