title: [The variation of PPAR pathway molecules in the lung tissue of rats under hyperbaric oxygen exposure].
abstract: OBJECTIVE
  To study the expression pattern of peroxisome proliferator-activated receptor (PPAR) pathway molecules in rat lung tissue under hyperbaric oxygen exposure.
  
  
  METHODS
  Twenty seven male SD rats were randomly divided into hyperbaric normoxia group (0.23 MPa air), hyperbaric oxygen treatment time series group (0.23 MPa oxygen, were exposed for 2 h, 4 h, 6 h or 8 h), continuous small flow of ventilation to maintain cabin O2 concentration > 99%. HE staining of lung tissue morphological changes and application oligo microarray to each time point lung were observed. Part of the PPAR pathway genes were validated by RT-PCR.
  
  
  RESULTS
  Compared with hyperbaric normoxia group, the lung injury caused by hyperbaric oxygen treatment gradually deteriorated during the time series. Expression microarray analysis of gene ontology (Go) enrichment analysis results in a class of PPAR pathway class included multiple PPAR pathway molecule. RT-PCR results suggested that PPAR-8 and PPAR-Y were up-regulated in the lung tissue after a long time exposure to hyperbaric oxygen.
  
  
  CONCLUSIONS
  Pro-longed hyperbaric oxygen exposure causing pulmonary oxygen toxicity can induce the activation of the PPAR pathway.
authors.affiliation: Naval Medical Research Institute, Shanghai 200433, China.
authors.forename: Pu
authors.initials: P
authors.lastname: You
authors.forename: Jian
authors.initials: J
authors.lastname: Yao
authors.forename: Xiao-Chen
authors.initials: XC
authors.lastname: Bao
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.forename: Shi
authors.initials: S
authors.lastname: Zhang
authors.forename: Yi-Qun
authors.initials: YQ
authors.lastname: Fang
mesh_terms: D000818:Animals; D006931:Hyperbaric Oxygenation / Q000009:adverse effects*; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008297:Male; D047492:Peroxisome Proliferator-Activated Receptors / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction
chemical_list: D047492:Peroxisome Proliferator-Activated Receptors