title: SPIB, a novel immunohistochemical marker for human blastic plasmacytoid dendritic cell neoplasms: characterization of its expression in major hematolymphoid neoplasms.
abstract: SPIB is an Ets transcription factor that is expressed exclusively in mature B cells, T-cell progenitors, and plasmacytoid dendritic cells. In the present study, we developed a novel mAb against the SPIB protein and characterized its expression in major hematolymphoid neoplasms, including a series of 45 cases of blastic plasmacytoid dendritic cell (BPDC) neoplasms and their potential cutaneous mimics. We found that SPIB is expressed heterogeneously among B- and T-cell lymphoma types. Interestingly, SPIB is expressed in a large proportion of nongerminal center type DLBCLs. In cutaneous neoplasms, SPIB is overexpressed in all BPDC neoplasms, but none of its cutaneous mimics. SPIB remains overexpressed in all cases that lack 1 or 2 of the markers used for BPDC neoplasms (ie, CD4, CD56, TCL1, and CD123). We conclude that SPIB expression can be used as a tool for diagnosing BPDC neoplasms, but it needs to be tested in conjunction with the growing arsenal of markers for human plasmacytoid dendritic cells.
authors.affiliation: Pathology Department, Hospital Universitario Marqués de Valdecilla, Instituto de Formación e Investigación Marqués de Valdecilla, Santander, Spain. smontes@humv.es
authors.forename: Santiago
authors.initials: S
authors.lastname: Montes-Moreno
authors.forename: Rocio
authors.initials: R
authors.lastname: Ramos-Medina
authors.forename: Azahara
authors.initials: A
authors.lastname: Martínez-López
authors.forename: Carlos
authors.initials: C
authors.lastname: Barrionuevo Cornejo
authors.forename: Alejandro
authors.initials: A
authors.lastname: Parra Cubillos
authors.forename: Shirley
authors.initials: S
authors.lastname: Quintana-Truyenque
authors.forename: Socorro María
authors.initials: SM
authors.lastname: Rodriguez Pinilla
authors.forename: Raquel
authors.initials: R
authors.lastname: Pajares
authors.forename: Lydia
authors.initials: L
authors.lastname: Sanchez-Verde
authors.forename: Jorge
authors.initials: J
authors.lastname: Martinez-Torrecuadrada
authors.forename: Giovanna
authors.initials: G
authors.lastname: Roncador
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Piris
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D003713:Dendritic Cells / Q000378:metabolism* / Q000473:pathology; D019337:Hematologic Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008221:Lymphoid Tissue / Q000378:metabolism / Q000473:pathology; D008223:Lymphoma / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D014157:Transcription Factors; C076877:SPIB protein, human