title: Interactions of legionella effector proteins with host phosphoinositide lipids.
abstract: By means of the Icm/Dot type IV secretion system Legionella pneumophila translocates several effector proteins into host cells, where they anchor to the cytoplasmic face of the LCV membrane by binding to phosphoinositide (PI) lipids. Thus, phosphatidylinositol-4-phosphate anchors the effector proteins SidC and SidM, which promote the interaction of LCVs with the ER and the secretory vesicle trafficking -pathway. In this chapter, we describe protocols to (1) identify PI-binding proteins in Legionella lysates using PI-beads, (2) determine PI-binding specificities and affinities of recombinant Legionella effector proteins by protein-lipid overlays, and (3) use Legionella effectors to identify cellular PI lipids.
authors.affiliation: Max von Pettenkofer Institute, Ludwig-Maximilians University, Munich, Germany.
authors.forename: Stephen
authors.initials: S
authors.lastname: Weber
authors.forename: Stephanie
authors.initials: S
authors.lastname: Dolinsky
authors.forename: Hubert
authors.initials: H
authors.lastname: Hilbi
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D058947:Bacterial Secretion Systems; D004023:Dictyostelium / Q000378:metabolism* / Q000382:microbiology*; D054884:Host-Pathogen Interactions; D007875:Legionella / Q000378:metabolism*; D016952:Legionella pneumophila / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D011485:Protein Binding; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D001426:Bacterial Proteins; D058947:Bacterial Secretion Systems; D010716:Phosphatidylinositols; D011993:Recombinant Fusion Proteins