title: Cell and tissue microarray technologies for protein and nucleic acid expression profiling.
abstract: Tissue microarray (TMA) and cell microarray (CMA) are two powerful techniques that allow for the immunophenotypical characterization of hundreds of samples simultaneously. In particular, the CMA approach is particularly useful for immunophenotyping new stem cell lines (e.g., cardiac, neural, mesenchymal) using conventional markers, as well as for testing the specificity and the efficacy of newly developed antibodies. We propose the use of a tissue arrayer not only to perform protein expression profiling by immunohistochemistry but also to carry out molecular genetics studies. In fact, starting with several tissues or cell lines, it is possible to obtain the complete signature of each sample, describing the protein, mRNA and microRNA expression, and DNA mutations, or eventually to analyze the epigenetic processes that control protein regulation. Here we show the results obtained using the Galileo CK4500 TMA platform.
authors.affiliation: Università Vita-Salute San Raffaele, Milano, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Cardano
authors.forename: Giuseppe R
authors.initials: GR
authors.lastname: Diaferia
authors.forename: Maurizio
authors.initials: M
authors.lastname: Falavigna
authors.forename: Chiara C
authors.initials: CC
authors.lastname: Spinelli
authors.forename: Fausto
authors.initials: F
authors.lastname: Sessa
authors.forename: Pasquale
authors.initials: P
authors.lastname: DeBlasio
authors.forename: Ida
authors.initials: I
authors.lastname: Biunno
mesh_terms: D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D016130:Immunophenotyping / Q000379:methods; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D009696:Nucleic Acids / Q000235:genetics* / Q000302:isolation & purification; D011506:Proteins / Q000032:analysis / Q000235:genetics; D046888:Tissue Array Analysis / Q000379:methods*
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D009696:Nucleic Acids; D011506:Proteins; C117384:SEL1L protein, human; C492169:Sel1h protein, mouse