title: High throughput characterization of structural differences between closely related proteins in solution.
abstract: Partitioning of a protein in an aqueous two-phase system (ATPS) is governed by interactions of the protein with aqueous media in the two phases. Here we describe how partitioning of proteins in a set of ATPS of different compositions can be used to quantify differences between 3D structures of closely related proteins. We also provide perspective on practical applications of the technology when comparative analysis of the higher-order structure of proteins is desired.
authors.affiliation: Analiza, Inc., 3516 Superior Ave., Suite 4407B, Cleveland, OH 44114, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Zaslavsky
authors.forename: Pedro
authors.initials: P
authors.lastname: Madeira
authors.forename: Leonid
authors.initials: L
authors.lastname: Breydo
authors.forename: Vladimir N
authors.initials: VN
authors.lastname: Uversky
authors.forename: Arnon
authors.initials: A
authors.lastname: Chait
authors.forename: Boris
authors.initials: B
authors.lastname: Zaslavsky
mesh_terms: D000818:Animals; D002417:Cattle; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013552:Swine
chemical_list: D011506:Proteins