title: NF-κB activation by equine arteritis virus is MyD88 dependent and promotes viral replication.
abstract: NF-κB, a family of transcription factors involved in different cell functions and immune responses is targeted by viruses. The mechanism of NF-κB signalling and its role in replication of EAV have not been investigated. We demonstrate that EAV infection in BHK-21 cells activates NF-κB, and this activation was found to be mediated through the MyD88 pathway. Infection of IKKβ(-/-) murine embryo fibroblasts (MEFs), which are deficient in NF-κB signalling, resulted in lower virus titre, less cytopathic effect, and reduced expression of viral proteins. These findings implicate the MyD88 pathway in EAV-induced NF-κB activation and suggest that NF-κB activation is essential for efficient replication of EAV.
authors.affiliation: Department of Virology, Immunobiology and Parasitology, National Veterinary Institute, Ulls Väg 2B, SE-751 89 Uppsala, Sweden.
authors.forename: Amin
authors.initials: A
authors.lastname: Mottahedin
authors.forename: Maruthibabu
authors.initials: M
authors.lastname: Paidikondala
authors.forename: Harindranath
authors.initials: H
authors.lastname: Cholleti
authors.forename: Claudia
authors.initials: C
authors.lastname: Baule
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D007240:Equartevirus / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D005347:Fibroblasts / Q000821:virology; D015967:Gene Expression Regulation, Viral; D051550:I-kappa B Kinase / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D051379:Mice; D053594:Myeloid Differentiation Factor 88 / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D015398:Signal Transduction; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics; D014779:Virus Replication
chemical_list: D053594:Myeloid Differentiation Factor 88; D016328:NF-kappa B; D014764:Viral Proteins; D051550:I-kappa B Kinase