title: Ca2+ microfluorimetry in retinal Müller glial cells.
abstract: Calcium acts as a prominent second messenger in virtually every cell type and modulates a plethora of cell functions. Thus, Ca(2+) microfluorimetry became a valuable tool to assess information about mechanisms involved in the regulation of the intracellular calcium level in research on living tissues. Here we offer insight into distinct approaches to detect changes in calcium levels specifically in Müller cells, the principal macroglial cells of the retina.
authors.affiliation: Paul Flechsig Institute for Brain Research, Department of Pathophysiology of Neuroglia, University of Leipzig, Leipzig, Germany.
authors.forename: Antje
authors.initials: A
authors.lastname: Wurm
authors.forename: Thomas
authors.initials: T
authors.lastname: Pannicke
authors.forename: Andreas
authors.initials: A
authors.lastname: Reichenbach
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D002469:Cell Separation / Q000379:methods; D004396:Coloring Agents / Q000032:analysis / Q000378:metabolism; D005470:Fluorometry / Q000379:methods*; D006168:Guinea Pigs; D009457:Neuroglia / Q000166:cytology* / Q000378:metabolism*; D012160:Retina / Q000166:cytology* / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods
chemical_list: D004396:Coloring Agents; D002118:Calcium