title: Parkin protein deficiency exacerbates cardiac injury and reduces survival following myocardial infarction.
abstract: It is known that loss-of-function mutations in the gene encoding Parkin lead to development of Parkinson disease. Recently, Parkin was found to play an important role in the removal of dysfunctional mitochondria via autophagy in neurons. Although Parkin is expressed in the heart, its functional role in this tissue is largely unexplored. In this study, we have investigated the role of Parkin in the myocardium under normal physiological conditions and in response to myocardial infarction. We found that Parkin-deficient (Parkin(-/-)) mice had normal cardiac function for up to 12 months of age as determined by echocardiographic analysis. Although ultrastructural analysis revealed that Parkin-deficient hearts had disorganized mitochondrial networks and significantly smaller mitochondria, mitochondrial function was unaffected. However, Parkin(-/-) mice were much more sensitive to myocardial infarction when compared with wild type mice. Parkin(-/-) mice had reduced survival and developed larger infarcts when compared with wild type mice after the infarction. Interestingly, Parkin protein levels and mitochondrial autophagy (mitophagy) were rapidly increased in the border zone of the infarct in wild type mice. In contrast, Parkin(-/-) myocytes had reduced mitophagy and accumulated swollen, dysfunctional mitochondria after the infarction. Overexpression of Parkin in isolated cardiac myocytes also protected against hypoxia-mediated cell death, whereas nonfunctional Parkinson disease-associated mutants ParkinR42P and ParkinG430D had no effect. Our results suggest that Parkin plays a critical role in adapting to stress in the myocardium by promoting removal of damaged mitochondria.
authors.affiliation: Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, California 92093-0758, USA.
authors.forename: Dieter A
authors.initials: DA
authors.lastname: Kubli
authors.forename: Xiaoxue
authors.initials: X
authors.lastname: Zhang
authors.forename: Youngil
authors.initials: Y
authors.lastname: Lee
authors.forename: Rita A
authors.initials: RA
authors.lastname: Hanna
authors.forename: Melissa N
authors.initials: MN
authors.lastname: Quinsay
authors.forename: Christine K
authors.initials: CK
authors.lastname: Nguyen
authors.forename: Rebecca
authors.initials: R
authors.lastname: Jimenez
authors.forename: Susanna
authors.initials: S
authors.lastname: Petrosyan
authors.forename: Anne N
authors.initials: AN
authors.lastname: Murphy
authors.forename: Asa B
authors.initials: AB
authors.lastname: Gustafsson
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004562:Electrocardiography; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D008929:Mitochondria, Heart / Q000502:physiology; D009203:Myocardial Infarction / Q000503:physiopathology*; D017207:Rats, Sprague-Dawley; D013534:Survival; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000502:physiology*
chemical_list: D044767:Ubiquitin-Protein Ligases; C111567:parkin protein