title: Real-time PCR investigation of the dynamic expression of three "RNA processing and modification" genes of Phanerochaete chrysosporium exposed to lead.
abstract: The expression of three ribosome binding proteins namely; polyadenylate-binding protein, splicing factor RNPS1 and ATP-dependent RNA helicase of Phanerochaete chrysosporium exposed to lead were analyzed by real-time PCR. The mRNA level of splicing factor RNPS1 showed 2.7 (p < 0.05), 2.6 (p < 0.05) and 4.9-fold (p < 0.001) increase when the cells were exposed to 25, 50 and 100 μM lead, respectively. 50 and 100 μM lead exposure resulted in almost 2-fold (p < 0.01and p < 0.05, respectively) increase in the expression of ATP-dependent RNA helicase. Polyadenylate-binding protein mRNA levels did not reveal any significant increase when cells exposed to the concentrations of lead employed. However, the mRNA level of polyadenylate-binding protein and splicing factor RNPS1 within a period of 1 and 2 h temporal exposure to 100 μM lead showed 2.5 (p < 0.001) and 3.4-fold (p < 0.001) increase, respectively. Expression level of ATP-dependent RNA helicase was not affected from the period of exposure to this metal.
authors.affiliation: Department of Biological Sciences, Middle East Technical University, 06800, Ankara, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Tekin
authors.forename: Bulent
authors.initials: B
authors.lastname: Icgen
authors.forename: Gulay
authors.initials: G
authors.lastname: Ozcengiz
mesh_terms: D004781:Environmental Exposure; D004784:Environmental Monitoring; D004785:Environmental Pollutants / Q000633:toxicity*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007854:Lead / Q000633:toxicity*; D020075:Phanerochaete / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D004785:Environmental Pollutants; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D007854:Lead