title: Inhibition of porcine reproductive and respiratory syndrome virus replication by flavaspidic acid AB.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) represents a significant challenge to the swine industry worldwide. Current control strategies against PRRSV are still inadequate and there is an urgent need for new antiviral therapies. Flavaspidic acid AB (FA-AB) is a compound derived from Dryopteris crassirhizoma, a traditional antiviral Chinese medicine. Here, we first identified its anti-PRRSV activity through targeting multiple stages in PRRSV infection in vitro. Our studies demonstrated that FA-AB could inhibit the internalization and cell-to-cell spreading of PRRSV, but not block PRRSV binding to cells. By monitoring the kinetics of PRRSV replication, we showed that FA-AB significantly suppressed PRRSV replication when treatment was initiated 24h after virus infection. Furthermore, we confirmed that FA-AB was able to significantly induce IFN-α, IFN-β, and IL1-β expression in porcine alveolar macrophages, suggesting that induction of antiviral cytokines by FA-AB could contribute to FA-AB induced inhibition of PRRSV replication. In conclusion, we provide a foundation for the possibility to develop a new therapeutic agent to control PRRSV infection.
authors.affiliation: State Key Laboratories of Agrobiotechnology, China Agricultural University, Beijing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.forename: Jianyong
authors.initials: J
authors.lastname: Si
authors.forename: Yipeng
authors.initials: Y
authors.lastname: Sun
authors.forename: Jinhua
authors.initials: J
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Cao
authors.forename: Wen-Hai
authors.initials: WH
authors.lastname: Feng
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002090:Butyrophenones / Q000302:isolation & purification / Q000494:pharmacology*; D002478:Cells, Cultured; D032492:Dryopteris / Q000737:chemistry; D015870:Gene Expression / Q000187:drug effects; D007155:Immunologic Factors / Q000302:isolation & purification / Q000494:pharmacology; D016899:Interferon-beta / Q000096:biosynthesis; D007371:Interferon-gamma / Q000096:biosynthesis; D053583:Interleukin-1beta / Q000096:biosynthesis; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D019316:Porcine respiratory and reproductive syndrome virus / Q000187:drug effects* / Q000502:physiology; D013552:Swine; D053586:Virus Internalization / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D002090:Butyrophenones; D007155:Immunologic Factors; D053583:Interleukin-1beta; D016899:Interferon-beta; C005000:flavaspidic acid; D007371:Interferon-gamma