title: Expression of transferrin receptor 1, proliferating cell nuclear antigen, p27(Kip1) and calbindin in the fetal and neonatal feline cerebellar cortex.
abstract: Cerebellar cortices from feline fetuses with estimated gestational ages of 40-66days and from kittens aged 2days to 2months, all negative for feline panleukopenia virus (FPV) infection, were analysed for expression of the transferrin receptor 1 (TrFR1), proliferating cell nuclear antigen (PCNA), p27(Kip1) and calbindin. TrFR1, the receptor used by FPV to enter target cells, was expressed in capillary endothelial cells in the cerebellum at all fetal stages investigated and in Purkinje cells of a 3-week-old kitten, but not in the neuroblasts in the external granule layer (EGL). PCNA was expressed in cells of the superficial layer of the EGL. The cyclin-dependent kinase inhibitor p27(Kip1) was expressed in cells of the deep layer of the EGL. Purkinje cells expressed calbindin from the earliest fetal stage investigated. Co-expression of PCNA and calbindin could not be demonstrated, indicating that feline Purkinje cells are post-mitotic from at least 40days gestation.
authors.affiliation: Laboratory of Anatomy, Biomechanics and Organogenesis, Faculty of Medicine, Université Libre de Bruxelles, 808 route de Lennik, B-1070 Brussels, Belgium. lcponce@ulb.ac.be
authors.forename: Luc
authors.initials: L
authors.lastname: Poncelet
authors.forename: Marie
authors.initials: M
authors.lastname: Springinsfeld
authors.forename: Kunie
authors.initials: K
authors.lastname: Ando
authors.forename: Céline
authors.initials: C
authors.lastname: Héraud
authors.forename: Anna
authors.initials: A
authors.lastname: Kabova
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Brion
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D064026:Calbindins / Q000235:genetics / Q000378:metabolism*; D002415:Cats / Q000196:embryology*; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000235:genetics / Q000378:metabolism*; D005333:Fetus / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D011689:Purkinje Cells / Q000378:metabolism; D011990:Receptors, Transferrin / Q000145:classification / Q000235:genetics / Q000378:metabolism*
chemical_list: D064026:Calbindins; D011990:Receptors, Transferrin; D050760:Cyclin-Dependent Kinase Inhibitor p27