title: [Changes in the mRNA expression of adiponectin, adiponectin receptors, and leptin in adipose tissue of Wannanhua pigs at different stages of development].
abstract: OBJECTIVE
  To explore the changes in the mRNA expression of adiponectin (Adp), adiponectin receptors(AdpR), and leptin in different adipose tissues of Wannanhua pigs at different stages of development, and their sexual dimorphism.
  
  
  METHODS
  Five Wannanhua boars and five Wannanhua gilts were sampled at birth, 30, 45, 90, and 180 days of age respectively. The delta delta Ct relative quantification real-time PCR was used to detect the transcription levels of Adp, AdpR1, AdpR2, and leptin mRNAs in subcutaneous (SC) and perirenal (PR) adipose tissues, and beta-actin were used as internal standards.
  
  
  RESULTS
  The expression level of Adp, AdpR1, AdpR2, and leptin mRNA in SC and PR adipose tissue were changed with age significantly (P < 0.01). In general, Adp mRNA expression in SC adipose tissue was significantly lower than that in PR adipose tissue (P < 0.05), while AdpR1, AdpR2, and leptin mRNA expression in SC adipose tissue were significantly higher than those in PR adipose tissue (P < 0.05 or P < 0.01). Although the sexual dimorphism were found in apart genes or apart days of age, Adp, AdpR1, AdpR2, and leptin mRNA expression both in SC adipose tissue and PR adipose tissue had no significant differences between Wannanhua gilts and boars in general. Significant positive correlation was found between Adp and AdpR1, AdpR2 (P < 0.05 or P < 0.01), and significant negative correlation was found between Adp and leptin (P < 0.05) in SC adipose tissue and PR adipose tissue respectively (P < 0.05).
  
  
  CONCLUSIONS
  The expression of Adp, AdpR1, AdpR2, and leptin mRNA in adipose tissue of Wannanhua pigs followed specific developmental patterns and tissue specificity. Adp correlated with its receptors.
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Sheng
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.forename: Jia
authors.initials: J
authors.lastname: Zhang
authors.forename: Kang
authors.initials: K
authors.lastname: Shao
authors.forename: Xiao-Xue
authors.initials: XX
authors.lastname: Wu
authors.forename: Wei-Xin
authors.initials: WX
authors.lastname: Li
authors.forename: Zong-Jun
authors.initials: ZJ
authors.lastname: Yin
mesh_terms: D000199:Actins / Q000378:metabolism; D052242:Adiponectin / Q000378:metabolism*; D000273:Adipose Tissue / Q000254:growth & development / Q000378:metabolism*; D000818:Animals; D005260:Female; D020738:Leptin / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D054419:Receptors, Adiponectin / Q000378:metabolism*; D013552:Swine
chemical_list: D000199:Actins; D052242:Adiponectin; D020738:Leptin; D012333:RNA, Messenger; D054419:Receptors, Adiponectin