title: Effects of siRNA targeting c-Myc and VEGF on human colorectal cancer Volo cells.
abstract: c-Myc and vascular endothelial growth factor (VEGF) genes are frequently deregulated and overexpressed in this malignancy, and strategies designed to inhibit c-Myc and VEGF expression in cancer cells may have considerable therapeutic value. In the present study, we design and use short interfering RNA (siRNA) to inhibit c-Myc and VEGF expression in colorectal cancer Volo cells and validate their effects on cell proliferation, cell cycle, apoptosis, and cell metastasis. Upon transient transfection with plasmid-encoding siRNA, it was found that expression of c-Myc and VEGF was significantly downregulated in siRNA-transfected cells and the downregulation of c-Myc and VEGF inhibited cell growth and induced apoptosis and metastasis of Volo cells. c-Myc and VEGF downregulation also increased cell population in the G0-G1 phase. In conclusion, the specific siRNA efficiently silenced the expression of c-Myc and VEGF, further suppressed the cell proliferation, triggered cell apoptosis, and inhibited cell invasiveness of colorectal cancer Volo cells.
authors.affiliation: Department of Colorectal Surgery, The First Hospital, Jilin University, Changchun, 130031, People's Republic of China.
authors.forename: Jiandong
authors.initials: J
authors.lastname: Tai
authors.forename: Guangyi
authors.initials: G
authors.lastname: Wang
authors.forename: Tongjun
authors.initials: T
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Chenghe
authors.initials: C
authors.lastname: Lin
authors.forename: Feng
authors.initials: F
authors.lastname: Li
mesh_terms: D017209:Apoptosis; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000473:pathology / Q000628:therapy*; D015316:Genetic Therapy; D006801:Humans; D009361:Neoplasm Invasiveness; D016271:Proto-Oncogene Proteins c-myc / Q000037:antagonists & inhibitors*; D034741:RNA, Small Interfering / Q000235:genetics*; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors*
chemical_list: C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc; D034741:RNA, Small Interfering; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A