title: Oligomerization of the carboxyl terminal domain of the human coronavirus 229E nucleocapsid protein.
abstract: The coronavirus (CoV) N protein oligomerizes via its carboxyl terminus. However, the oligomerization mechanism of the C-terminal domains (CTD) of CoV N proteins remains unclear. Based on the protein disorder prediction system, a comprehensive series of HCoV-229E N protein mutants with truncated CTD was generated and systematically investigated by biophysical and biochemical analyses to clarify the role of the C-terminal tail of the HCoV-229E N protein in oligomerization. These results indicate that the last C-terminal tail plays an important role in dimer-dimer association. The C-terminal tail peptide is able to interfere with the oligomerization of the CTD of HCoV-229E N protein and performs the inhibitory effect on viral titre of HCoV-229E. This study may assist the development of anti-viral drugs against HCoV.
authors.affiliation: Institute of Genomics and Bioinformatics, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Yu-Sheng
authors.initials: YS
authors.lastname: Lo
authors.forename: Shing-Yen
authors.initials: SY
authors.lastname: Lin
authors.forename: Shiu-Mei
authors.initials: SM
authors.lastname: Wang
authors.forename: Chin-Tien
authors.initials: CT
authors.lastname: Wang
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Chiu
authors.forename: Tai-Huang
authors.initials: TH
authors.lastname: Huang
authors.forename: Ming-Hon
authors.initials: MH
authors.lastname: Hou
mesh_terms: D002460:Cell Line; D002942:Circular Dichroism; D028941:Coronavirus 229E, Human / Q000737:chemistry* / Q000235:genetics; D000086462:Coronavirus Nucleocapsid Proteins; D006801:Humans; D019590:Nucleocapsid Proteins / Q000737:chemistry* / Q000235:genetics; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D011487:Protein Conformation; D055503:Protein Multimerization; D055550:Protein Stability; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D000086462:Coronavirus Nucleocapsid Proteins; D019590:Nucleocapsid Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins