title: Simple and sensitive method for measurement of metoclopramide in cattle plasma by LC-MS/MS using a multimode chromatography.
abstract: A quantitative assay method using LC/ESI-MS/MS for simultaneous determination of MCP in cattle plasma was developed and validated. Chromatographic separation was carried out using a multimode column (2 × 150 mm, 3 μm) with gradient elution (0.05% formic acid/methanol with 0.05% formic acid). MCP and levosulpiride (internal standard) were analyzed in the precursor/product ion pair of m/z 300.1/226.9 and 342.0/112.0, respectively. Linear calibration curves were obtained in the range of 2.5-500 ng/ml (R(2)>0.999) with a lower limit of quantification of 0.05 ng/ml. Mean recoveries were 96-103%, and the coefficient of variation was less than 6.5%. Plasma MCP concentrations after intravenous administration at 0.4 mg/kg to 12 cattle were determined by the LC-MS/MS method.
authors.affiliation: National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1 Tokura, Kokubunji, Tokyo, Japan. hamamok@nval.maff.go.jp
authors.forename: Kouko
authors.initials: K
authors.lastname: Hamamoto
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Mizuno
authors.forename: Masaki
authors.initials: M
authors.lastname: Kato
authors.forename: Norio
authors.initials: N
authors.lastname: Yamagishi
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Furuhama
mesh_terms: D000818:Animals; D002417:Cattle / Q000097:blood* / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods*; D018492:Dopamine Antagonists / Q000097:blood* / Q000493:pharmacokinetics; D057230:Limit of Detection; D008787:Metoclopramide / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D018492:Dopamine Antagonists; D008787:Metoclopramide