title: Cutting edge: B cells are essential for protective immunity against Salmonella independent of antibody secretion.
abstract: Typhoid fever and nontyphoidal bacteremia caused by Salmonella remain critical human health problems. B cells are required for protective immunity to Salmonella, but the mechanism of protection remains unclear. In this study, we immunized wild-type, B cell-deficient, Ab-deficient, and class-switched Ab-deficient mice with attenuated Salmonella and examined protection against secondary infection. As expected, wild-type mice were protected and B cell-deficient mice succumbed to secondary infection. Interestingly, mice with B cells but lacking secreted Ab or class-switched Ab had little deficiency in resistance to Salmonella infection. The susceptibility of B cell-deficient mice correlated with marked reductions in CD4 T cell IFN-γ production after secondary infection. Taken together, these data suggest that the primary role of B cells in acquired immunity to Salmonella is via the development of protective T cell immunity.
authors.affiliation: Department of Pediatric Infectious Disease, Center for Infectious Diseases and Microbiology Translational Research, University of Minnesota Medical School-Twin Cities, Minneapolis, MN 55455, USA. nanto001@umn.edu
authors.forename: Minelva R
authors.initials: MR
authors.lastname: Nanton
authors.forename: Sing Sing
authors.initials: SS
authors.lastname: Way
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Shlomchik
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: McSorley
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D016175:B-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D007111:Immunity, Cellular; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D008822:Mice, Transgenic; D012480:Salmonella Infections / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D012486:Salmonella typhimurium / Q000276:immunology* / Q000472:pathogenicity; D018417:Th1 Cells / Q000276:immunology / Q000382:microbiology; D014774:Virulence / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial