title: The expression pattern of classical MHC class I molecules in the development of mouse central nervous system.
abstract: Classical major histocompatibility complex (MHC) class I, first identified in the immune system, is also expressed in the developing and adult central nervous system (CNS). Although the MHC class I molecules have been found to be expressed in the CNS of different species, a necessary step to elucidate the temporal and spatial expression patterns of MHC class I molecules in the brain development has never been taken. Frozen sections were made from the brains of embryonic and postnatal C57BL/6 J mice, and the expression of H-2D(b) mRNA was examined by in situ hybridization. Immunofluorescence was also performed to define the cell types that express H2-D(b) in P15 mice. At E10.5, the earliest stage we examined, H2-D(b) was expressed in neuroepithelium of the brain vesicles. From E12.5 to P0, H2-D(b) expression was mainly located at cerebral cortex, neuroepithelium of the lateral ventricle, neuroepithelium of aquaeductus and developing cerebellum. From P4 to adult, H2-D(b) mRNA was detected at olfactory bulb, hippocampus, cerebellum and some nerve nuclei. The major cell types expressing H-2D(b) in P15 hippocampus, cerebral cortex and olfactory bulb were neuron. H2-K(b) signal paralleled that of H2-D(b) and the expression levels of the two molecules were comparable throughout the brain. The investigation of the expression pattern of H-2D(b) at both embryonic and postnatal stages is important for further understanding the physiological and pathological roles of H2-D(b) in the developing CNS.
authors.affiliation: Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Department of Microbiology and Immunology, Medical School, Southeast University, 87 Dingjiaqiao Road, 210009 Nanjing, China.
authors.forename: Jiane
authors.initials: J
authors.lastname: Liu
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Shen
authors.forename: Mingli
authors.initials: M
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Shi
authors.forename: Aifeng
authors.initials: A
authors.lastname: Zhang
authors.forename: Fengqin
authors.initials: F
authors.lastname: Miao
authors.forename: Junhua
authors.initials: J
authors.lastname: Liu
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Wu
authors.forename: Youji
authors.initials: Y
authors.lastname: He
authors.forename: Jianqiong
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000375:Aging / Q000235:genetics; D000818:Animals; D001921:Brain / Q000196:embryology / Q000254:growth & development* / Q000276:immunology*; D002490:Central Nervous System / Q000196:embryology / Q000254:growth & development / Q000276:immunology; D018507:Gene Expression Regulation, Developmental / Q000276:immunology*; D006183:H-2 Antigens / Q000096:biosynthesis / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000276:immunology / Q000378:metabolism
chemical_list: D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D015395:Histocompatibility Antigens Class I