title: [Alisol B inhibited complement 3a-induced human renal tubular epithelial to mesenchymal transition].
abstract: OBJECTIVE
  To study whether alisol B could inhibit complement 3a (C3a) induced renal tubular epithelial-mesenchymal transition (EMT).
  
  
  METHODS
  The in vitro cultured human renal tubular epithelial HK-2 cells were intervened with 5 ng/mL transforming growth factor-beta (TGF-beta), 0.1 micromol C3a, and 0.1 micromol C3a + 10 micromol alisol B, respectively. The mRNA and protein expressions of alpha-SMA, E-cadherin, and C3 were detected using RT-PCR, Western blot, and immunofluorescence, respectively.
  
  
  RESULTS
  The mRNA and protein expressions of C3 in HK-2 cells were up-regulated after intervention of C3a (P < 0.01), the mRNA and protein expressions of alpha-SMA in HK-2 cells were obviously enhanced (P < 0.01), the mRNA and protein expressions of E-cadherin obviously decreased (P < 0.01). When compared with the group intervened by exogenous C3a, after intervention of alisol B, the mRNA and protein expressions of alpha-SMA in HK-2 cells were obviously reduced (P < 0.01), the mRNA and protein expressions of E-cadherin obviously increased (P < 0.05).
  
  
  CONCLUSIONS
  Exogenous C3a could induce renal tubular EMT. Alisol B was capable of suppressing C3a induced EMT.
authors.affiliation: Department of Nephrology, First Affiliated Hospital of Fujian Medical University, Fuzhou.
authors.forename: Rui-fang
authors.initials: RF
authors.lastname: Zhang
authors.forename: Jian-xin
authors.initials: JX
authors.lastname: Wan
authors.forename: Yan-fang
authors.initials: YF
authors.lastname: Xu
mesh_terms: D000199:Actins / Q000378:metabolism; D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002783:Cholestenones / Q000494:pharmacology*; D015926:Complement C3a / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D006801:Humans; D007684:Kidney Tubules / Q000166:cytology* / Q000378:metabolism
chemical_list: D000199:Actins; D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; D002783:Cholestenones; C457232:alisol B; D015926:Complement C3a