title: Overexpression of OsRecQl4 and/or OsExo1 enhances DSB-induced homologous recombination in rice.
abstract: During homologous recombination (HR)-mediated DNA double-strand break (DSB) repair in eukaryotes, an initial step is the creation of a 3'-single-stranded DNA (ssDNA) overhang via resection of a 5' end. Rad51 polymerizes on this ssDNA to search for a homologous sequence, and the gapped sequence is then repaired using an undamaged homologous DNA strand as template. Recent studies in eukaryotes indicate that resection of the DSB site is promoted by the cooperative action of RecQ helicase family proteins: Bloom helicase (BLM) in mammals or Sgs1 in yeast, and exonuclease 1 (Exo1). However, the role of RecQ helicase and exonuclease during the 5'-resection process of HR in plant cells has not yet been defined. Here, we demonstrate that overexpression of rice proteins OsRecQl4 (BLM counterpart) and/or OsExo1 (Exo1 homolog) can enhance DSB processing, as evaluated by recombination substrate reporter lines in rice. These results could be applied to construct an efficient gene targeting system in rice.
authors.affiliation: Plant Genome Engineering Research Unit, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602 Japan.
authors.forename: Yong-Ik
authors.initials: YI
authors.lastname: Kwon
authors.forename: Kiyomi
authors.initials: K
authors.lastname: Abe
authors.forename: Keishi
authors.initials: K
authors.lastname: Osakabe
authors.forename: Masaki
authors.initials: M
authors.lastname: Endo
authors.forename: Ayako
authors.initials: A
authors.lastname: Nishizawa-Yokoi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Saika
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Shimada
authors.forename: Seiichi
authors.initials: S
authors.lastname: Toki
mesh_terms: D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000235:genetics / Q000378:metabolism; D005090:Exodeoxyribonucleases / Q000235:genetics* / Q000378:metabolism; D017930:Genes, Reporter; D005819:Genetic Markers; D005966:Glucuronidase; D059765:Homologous Recombination / Q000235:genetics*; D008958:Models, Molecular; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D053484:RecQ Helicases / Q000235:genetics* / Q000378:metabolism; D036226:Seedlings / Q000201:enzymology / Q000235:genetics
chemical_list: D018744:DNA, Plant; D004277:DNA, Single-Stranded; D005819:Genetic Markers; D010940:Plant Proteins; D005090:Exodeoxyribonucleases; C028450:exodeoxyribonuclease I; D005966:Glucuronidase; D053484:RecQ Helicases