title: ¹H, ¹³C, and ¹⁵N backbone and side chain resonance assignments of the C-terminal DNA binding and dimerization domain of v-Myc.
abstract: The oncogenic transcription factor Myc is one of the most interesting members of the basic-helix-loop-helix-zipper (bHLHZip) protein family. Deregulation of Myc via gene amplification, chromosomal translocation or other mechanisms lead to tumorigenesis including Burkitt lymphoma, multiple myeloma, and many other malignancies. The oncogene myc is a highly potent transforming gene and capable to transform various cell types in vivo and in vitro. Its oncogenic activity initialized by deregulated expression leads to a shift of the equilibrium in the Myc/Max/Mad network towards Myc/Max complexes. The Myc/Max heterodimerization is a prerequisite for transcriptional functionality of Myc. Primarily, we are focusing on the apo-state of the C-terminal domain of v-Myc, the retroviral homolog of human c-Myc. Based on multi-dimensional NMR measurements v-Myc appears to be neither a fully structured nor a completely unstructured protein. The bHLHZip domain of v-Myc does not exist as a random coil but exhibits partially pre-formed α-helical regions in its apo-state. In order to elucidate the structural propensities of Myc in more detail, the backbone and side-chain assignments obtained here for apo-Myc are a crucial prerequisite for further NMR measurements.
authors.affiliation: Max F. Perutz Laboratories, Department of Structural and Computational Biology, University of Vienna, Vienna Biocenter Campus 5, 1030 Vienna, Austria.
authors.forename: Gönül
authors.initials: G
authors.lastname: Kızılsavaş
authors.forename: Saurabh
authors.initials: S
authors.lastname: Saxena
authors.forename: Szymon
authors.initials: S
authors.lastname: Żerko
authors.forename: Wiktor
authors.initials: W
authors.lastname: Koźmiński
authors.forename: Klaus
authors.initials: K
authors.lastname: Bister
authors.forename: Robert
authors.initials: R
authors.lastname: Konrat
mesh_terms: D000595:Amino Acid Sequence; D002247:Carbon Isotopes; D004247:DNA / Q000378:metabolism*; D006801:Humans; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D016271:Proto-Oncogene Proteins c-myc / Q000737:chemistry* / Q000378:metabolism*; D011522:Protons
chemical_list: D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D016271:Proto-Oncogene Proteins c-myc; D011522:Protons; D004247:DNA