title: [Effects of CGRP on the E-cadherin expression in human bronchial epithelial cells].
abstract: OBJECTIVE
  To discuss the effect of calcitonin gene-related peptides (CGRP) on epithelial cadherin (E-cd) expression in human bronchial epithelial cells (HBECs) in vitro.
  
  
  METHODS
  The effect of CGRP on E-cd protein and mRNA expression in both normal and O3-challenged HBECs were determined by immunocytochemistry and RT-PCR. The signal transduction pathways of CGRP were observed by using protein kinase C(PKC) inhibitor (H-7), calmodulin(CaM) inhibitor (W-7) and PKA inhibitor (H-89).
  
  
  RESULTS
  CGRP increased E-cd mRNA and protein expressions of normal and O3-challenged HBECs in a dose-dependent manner. CGRP had no effect on cytoplasm E-cd expression. Pre-treatment with H-89, H-7 and W-7, the up-regulatory effect of CGRP on E-cd expression was partly abolished.
  
  
  CONCLUSIONS
  CGRP increased in cytomembrane E-cd expression of normal and O3-challenged HBECs in a dose-dependent manner. E-cd expression on HBECs was strengthened by CGRP via PKA, PKC and CaM pathways.
authors.affiliation: Department of Physiology, Xiangya Medical College, Central South University, Changsha 410008, China.
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Bai
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Lu
authors.forename: Jia-Xi
authors.initials: JX
authors.lastname: Duan
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhou
authors.forename: Guo-Ying
authors.initials: GY
authors.lastname: Sun
authors.forename: Cha-Xiang
authors.initials: CX
authors.lastname: Guan
mesh_terms: D015703:Antigens, CD; D001980:Bronchi / Q000166:cytology; D015820:Cadherins / Q000378:metabolism*; D015740:Calcitonin Gene-Related Peptide / Q000008:administration & dosage / Q000494:pharmacology*; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D010126:Ozone; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; D012333:RNA, Messenger; D010126:Ozone; D015740:Calcitonin Gene-Related Peptide