title: Marinicauda pacifica gen. nov., sp. nov., a prosthecate alphaproteobacterium of the family Hyphomonadaceae isolated from deep seawater.
abstract: A marine prosthecate bacterium, designated strain P-1 km-3(T), was isolated from deep seawater from the Pacific. Cells of strain P-1 km-3(T) were Gram-stain-negative, aerobic, catalase- and oxidase-positive, dimorphic rods with a single polar prostheca or flagellum. The strain hydrolysed gelatin and grew at 6-40 °C (optimum, 30 °C) and with 0.5-12% (w/v) NaCl (optimum, 2%). Phylogenetic analysis of the 16S rRNA gene sequences revealed that strain P-1 km-3(T) belonged to the family Hyphomonadaceae in the class Alphaproteobacteria and represented a separate lineage, located between the genera Oceanicaulis and Woodsholea. Sequence similarities of strain P-1 km-3(T) with type strains of species of the genera Oceanicaulis and Woodsholea were 93.2-93.9%. The predominant cellular fatty acids in strain P-1 km-3(T) were C18:1ω7c, C18:0, 11-methyl C18:1ω7c, C17:0 and C19:0 cyclo ω8c. The major respiratory quinone of strain P-1 km-3(T) was Q-10. The polar lipids of strain P-1 km-3(T) comprised glucuronopyranosyldiglyceride (GUDG), monoglycosyldiglyceride (MGDG), sulfo-quinovosyl diacylglycerol (SQDG), phosphatidylglycerol (PG), an unidentified phospholipid (PL) and an unidentified lipid (L). The genomic DNA G+C content of strain P-1 km-3(T) was 66.0 mol%. On the basis of the polyphasic data presented in this study, strain P-1 km-3(T) is proposed to represent a novel species in a new genus, Marinicauda pacifica gen. nov., sp. nov., within the family Hyphomonadaceae. The type strain of the type species is P-1 km-3(T) (=KACC 16526(T)=CGMCC 1.11031(T)).
authors.affiliation: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors.forename: Xi-Ying
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors.forename: Guo-Wei
authors.initials: GW
authors.lastname: Li
authors.affiliation: Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.|Laboratory of Marine Ecosystem and Biogeochemistry, State Oceanic Administration, Hangzhou 310012, PR China.
authors.forename: Chun-Sheng
authors.initials: CS
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors.forename: Yan-Jiao
authors.initials: YJ
authors.lastname: Zhang
authors.affiliation: Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.|Laboratory of Marine Ecosystem and Biogeochemistry, State Oceanic Administration, Hangzhou 310012, PR China.
authors.forename: Xue-Wei
authors.initials: XW
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors.forename: Hai
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors.forename: Ang
authors.initials: A
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors.forename: Bin-Bin
authors.initials: BB
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors.forename: Qi-Long
authors.initials: QL
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors.forename: Xiu-Lan
authors.initials: XL
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors.forename: Bai-Cheng
authors.initials: BC
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors.forename: Yu-Zhong
authors.initials: YZ
authors.lastname: Zhang
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010140:Pacific Ocean; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S