title: miR-137 restoration sensitizes multidrug-resistant MCF-7/ADM cells to anticancer agents by targeting YB-1.
abstract: Multidrug resistance (MDR) to chemotherapeutic agents is a major obstacle to successful treatment in breast cancer patients. The aims of this study were to investigate whether miR-137 was involved in the regulation of MDR, and to explore the mechanism of miR-137 on the sensitivity of MCF-7/ADM cells. miR-137 was downregulated in MCF-7/ADM cells, and its expression was found to inversely correlate with Y-box binding protein-1 (YB-1) and P-glycoprotein (P-gp) levels in breast cancer cells. Furthermore, YB-1 was confirmed as a target of miR-137 by luciferase reporter assay and western blot analysis. Moreover, elevated expression of miR-137 reduced the protein expression levels of YB-1 and P-gp, mimicking the effect of YB-1 knockdown in the sensitivity of MCF-7/ADM cells to anticancer agents, whereas restoration of YB-1 diminished this effect. In conclusion, our results demonstrated that miR-137 was involved in MDR in cancer through modulation of P-gp by targeting YB-1, suggesting that miR-137 might be a potential target for preventing and reversing MDR in tumor cells.
authors.affiliation: The Fourth Affiliated Hospital of Jiangsu University, Zhenjiang 212001,  China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Zhu
authors.forename: Yuefeng
authors.initials: Y
authors.lastname: Li
authors.forename: Huiling
authors.initials: H
authors.lastname: Shen
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Lulu
authors.initials: L
authors.lastname: Long
authors.forename: Lulu
authors.initials: L
authors.lastname: Hui
authors.forename: Wenlin
authors.initials: W
authors.lastname: Xu
mesh_terms: D000970:Antineoplastic Agents / Q000627:therapeutic use*; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000378:metabolism*; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D016896:Treatment Outcome; D051840:Y-Box-Binding Protein 1 / Q000037:antagonists & inhibitors* / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; C545567:MIRN137 microRNA, human; D035683:MicroRNAs; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human