title: Quantitative microfluidic analysis of S- and R-type endotoxin components with chip capillary electrophoresis.
abstract: A novel, fast, and sensitive ME method was developed to analyze and differentiate the smooth (S) and rough (R) type bacterial endotoxin components labeled covalently with a fluorescent dye. The quantitative analysis of purified lipopolysaccharides, or partially purified samples from whole-cell lysates becomes possible with this method. Two groups with three sub-groups in the first group of S-type lipopolysaccharides can be classified based on the electrophoretic profiles. The LOD of the endotoxins from S- and R-type Gram-negative bacteria was found to be 2.6 ng and 6.9 ng, respectively. This method is capable to replace the commonly used SDS-PAGE combined with silver staining.
authors.affiliation: Institute of Bioanalysis, Faculty of Medicine, University of Pécs, Pécs, Hungary.
authors.forename: Lilla
authors.initials: L
authors.lastname: Makszin
authors.forename: Anikó
authors.initials: A
authors.lastname: Kilár
authors.forename: Péter
authors.initials: P
authors.lastname: Felső
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Péterfi
authors.forename: Béla
authors.initials: B
authors.lastname: Kocsis
authors.forename: Ferenc
authors.initials: F
authors.lastname: Kilár
mesh_terms: D046209:Electrophoresis, Microchip / Q000379:methods*; D004755:Enterobacteriaceae / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D057230:Limit of Detection; D008070:Lipopolysaccharides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D008970:Molecular Weight; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013237:Stereoisomerism
chemical_list: D005456:Fluorescent Dyes; D008070:Lipopolysaccharides