title: A forward genetic screen identifies eukaryotic translation initiation factor 3, subunit H (eIF3h), as an enhancer of variegation in the mouse.
abstract: We have used a forward genetic screen to identify genes required for transgene silencing in the mouse. Previously these genes were found using candidate-based sequencing, a slow and labor-intensive process. Recently, whole-exome deep sequencing has accelerated our ability to find the causative point mutations, resulting in the discovery of novel and sometimes unexpected genes. Here we report the identification of translation initiation factor 3, subunit H (eIF3h) in two modifier of murine metastable epialleles (Mommes) lines. Mice carrying mutations in this gene have not been reported previously, and a possible involvement of eIF3h in transcription or epigenetic regulation has not been considered.
authors.affiliation: Epigenetics Laboratory, Queensland Institute of Medical Research, Herston, Brisbane, QLD 4006, Australia.
authors.forename: Lucia
authors.initials: L
authors.lastname: Daxinger
authors.forename: Harald
authors.initials: H
authors.lastname: Oey
authors.forename: Anwyn
authors.initials: A
authors.lastname: Apedaile
authors.forename: Joanne
authors.initials: J
authors.lastname: Sutton
authors.forename: Alyson
authors.initials: A
authors.lastname: Ashe
authors.forename: Emma
authors.initials: E
authors.lastname: Whitelaw
mesh_terms: D000818:Animals; D055012:Chromosomal Position Effects; D039621:Eukaryotic Initiation Factor-3 / Q000235:genetics*; D059472:Exome / Q000235:genetics; D020868:Gene Silencing; D005820:Genetic Testing; D059014:High-Throughput Nucleotide Sequencing; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D021122:Protein Subunits / Q000235:genetics*; D017422:Sequence Analysis, DNA; D019076:Transgenes
chemical_list: D039621:Eukaryotic Initiation Factor-3; D021122:Protein Subunits