title: Effects of poly(L-lysine)-modified Fe3O4 nanoparticles on endogenous reactive oxygen species in cancer stem cells.
abstract: Intracellular reactive oxygen species (ROS) have been extensively shown to play an important role in the regulation of cell proliferation and cell cycle progression. The effects of endogenous ROS on the proliferation and differentiation of cancer stem cells (CSCs) have received increasing attention because of the unique properties of these cells that allow them to drive tumor growth and evade conventional cancer therapies. In this study, poly(L-Lysine) (PLL)-modified Fe(3)O(4) nanoparticles were synthesized to label CSCs derived from U251 glioblastoma multiform. A featured peroxidase-like activity within PLL-modified Fe(3)O(4) nanoparticles that could greatly reduce intracellular H(2)O(2) activity was identified. We also found that PLL-modified Fe(3)O(4) nanoparticles could accelerate the progression of CSC cell cycle, probably due to the impaired activity of endogenous ROS in CSCs. These results show that growth and proliferation of CSCs could be promoted by Fe(3)O(4) nanocarriers in an ROS-dependent manner, and Fe(3)O(4) nanocarriers may be suitable for certain tumor therapies as a drug delivery system.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Xueqin
authors.initials: X
authors.lastname: Wang
authors.forename: Qin
authors.initials: Q
authors.lastname: Tu
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.forename: Yanfei
authors.initials: Y
authors.lastname: An
authors.forename: Jian-Chun
authors.initials: JC
authors.lastname: Wang
authors.forename: Wenming
authors.initials: W
authors.lastname: Liu
authors.forename: Mao-Sen
authors.initials: MS
authors.lastname: Yuan
authors.forename: Saeed Mahmoud
authors.initials: SM
authors.lastname: Ahmed
authors.forename: Juan
authors.initials: J
authors.lastname: Xu
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.forename: Yanrong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jinyi
authors.initials: J
authors.lastname: Wang
mesh_terms: D045744:Cell Line, Tumor; D005909:Glioblastoma / Q000378:metabolism*; D006801:Humans; D058185:Magnetite Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D011107:Polylysine / Q000008:administration & dosage* / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D058185:Magnetite Nanoparticles; D017382:Reactive Oxygen Species; D011107:Polylysine