title: Influence of red pepper spice and turmeric on inflammation and oxidative stress biomarkers in overweight females: a metabolomics approach.
abstract: Red pepper spice (RP) and turmeric (TM) are used as flavorings in foods and for medicinal purposes. Utilizing a randomized, doubled-blinded, placebo-controlled, crossover design (2-week washout), 4-week supplementation with RP (1 g/d) or TM (2.8 g/d) was tested for influences on inflammation and oxidative stress in 62 overweight/obese (body mass index ≥ 27 kg/m²) females (40-75 years) with systemic inflammation (C-reactive protein, CRP ≥ 2 mg/l). Overnight, fasted blood samples were collected pre- and post-supplementation, and analyzed for oxidative stress (F₂-isoprostanes, oxidized low density lipoprotein), inflammation (CRP and seven inflammatory cytokines), and metabolic profiles using gas chromatography-mass spectrometry with multivariate partial least square discriminant analysis (PLS-DA). Pre- to post-supplementation measures of inflammation and oxidative stress for both RP and TM did not differ when compared to placebo (all interaction effects, P > 0.05), and global metabolic difference scores calculated through PLS-DA were non-significant (both spices, Q²Y < 0.40). These data indicate that 4-week supplementation with RP or TM at culinary levels does not alter oxidative stress or inflammation in overweight/obese females with systemic inflammation, or cause a significant shift in the global metabolic profile.
authors.affiliation: Human Performance Lab, North Carolina Research Campus, Appalachian State University, Kannapolis, NC 28081, USA. niemandc@appstate.edu
authors.forename: David C
authors.initials: DC
authors.lastname: Nieman
authors.forename: Lynn
authors.initials: L
authors.lastname: Cialdella-Kam
authors.forename: Amy M
authors.initials: AM
authors.lastname: Knab
authors.forename: R Andrew
authors.initials: RA
authors.lastname: Shanely
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D001794:Blood Pressure; D001823:Body Composition / Q000187:drug effects; D015992:Body Mass Index; D002097:C-Reactive Protein / Q000378:metabolism; D002212:Capsicum / Q000737:chemistry*; D018592:Cross-Over Studies; D030024:Curcuma / Q000737:chemistry*; D016207:Cytokines / Q000097:blood; D019587:Dietary Supplements; D004311:Double-Blind Method; D028441:F2-Isoprostanes / Q000097:blood; D005260:Female; D006801:Humans; D007249:Inflammation / Q000517:prevention & control; D008077:Lipoproteins, LDL / Q000097:blood; D055432:Metabolomics / Q000379:methods*; D008875:Middle Aged; D050177:Overweight / Q000097:blood / Q000188:drug therapy; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D017365:Spices
chemical_list: D015415:Biomarkers; D016207:Cytokines; D028441:F2-Isoprostanes; D008077:Lipoproteins, LDL; D010936:Plant Extracts; C099252:oxidized low density lipoprotein; C012596:turmeric extract; D002097:C-Reactive Protein