title: Human amniotic fluid stem cell-derived muscle progenitor cell therapy for stress urinary incontinence.
abstract: The most promising treatment for stress urinary incontinence can be a cell therapy. We suggest human amniotic fluid stem cells (hAFSCs) as an alternative cell source. We established the optimum in vitro protocol for the differentiation from hAFSCs into muscle progenitors. These progenitors were transplanted into the injured urethral sphincter and their therapeutic effect was analyzed. For the development of an efficient differentiation system in vitro, we examined a commercial medium, co-culture and conditioned medium (CM) systems. After being treated with CM, hAFSCs were effectively developed into a muscle lineage. The progenitors were integrated into the host urethral sphincter and the host cell differentiation was stimulated in vivo. Urodynamic analysis showed significant increase of leak point pressure and closing pressure. Immunohistochemistry revealed the regeneration of circular muscle mass with normal appearance. Molecular analysis observed the expression of a larger number of target markers. In the immunogenicity analysis, the progenitor group had a scant CD8 lymphocyte. In tumorigenicity, the progenitors showed no teratoma formation. These results suggest that hAFSCs can effectively be differentiated into muscle progenitors in CM and that the hAFSC-derived muscle progenitors are an accessible cell source for the regeneration of injured urethral sphincter.
authors.affiliation: Joint Institute for Regenerative Medicine, Kyungpook National University Hospital, Daegu, Korea.
authors.forename: So Young
authors.initials: SY
authors.lastname: Chun
authors.forename: Deok Hyun
authors.initials: DH
authors.lastname: Cho
authors.forename: Seon Yeong
authors.initials: SY
authors.lastname: Chae
authors.forename: Kyung Hee
authors.initials: KH
authors.lastname: Choi
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Lim
authors.forename: Ghil Suk
authors.initials: GS
authors.lastname: Yoon
authors.forename: Bum Soo
authors.initials: BS
authors.lastname: Kim
authors.forename: Bup Wan
authors.initials: BW
authors.lastname: Kim
authors.forename: James J
authors.initials: JJ
authors.lastname: Yoo
authors.forename: Tae Gyun
authors.initials: TG
authors.lastname: Kwon
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D018920:Coculture Techniques; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008813:Mice, Inbred ICR; D012038:Regeneration; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014521:Urethra / Q000502:physiology; D014550:Urinary Incontinence, Stress / Q000473:pathology / Q000628:therapy*; D014563:Urodynamics
chemical_list: D015415:Biomarkers