title: Tubular structure induced by a plant virus facilitates viral spread in its vector insect.
abstract: Rice dwarf virus (RDV) replicates in and is transmitted by a leafhopper vector in a persistent-propagative manner. Previous cytopathologic and genetic data revealed that tubular structures, constructed by the nonstructural viral protein Pns10, contain viral particles and are directly involved in the intercellular spread of RDV among cultured leafhopper cells. Here, we demonstrated that RDV exploited these virus-containing tubules to move along actin-based microvilli of the epithelial cells and muscle fibers of visceral muscle tissues in the alimentary canal, facilitating the spread of virus in the body of its insect vector leafhoppers. In cultured leafhopper cells, the knockdown of Pns10 expression due to RNA interference (RNAi) induced by synthesized dsRNA from Pns10 gene strongly inhibited tubule formation and prevented the spread of virus among insect vector cells. RNAi induced after ingestion of dsRNA from Pns10 gene strongly inhibited formation of tubules, preventing intercellular spread and transmission of the virus by the leafhopper. All these results, for the first time, show that a persistent-propagative virus exploits virus-containing tubules composed of a nonstructural viral protein to traffic along actin-based cellular protrusions, facilitating the intercellular spread of the virus in the vector insect. The RNAi strategy and the insect vector cell culture provide useful tools to investigate the molecular mechanisms enabling efficient transmission of persistent-propagative plant viruses by vector insects.
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.forename: Hongyan
authors.initials: H
authors.lastname: Chen
authors.forename: Qianzhuo
authors.initials: Q
authors.lastname: Mao
authors.forename: Qifei
authors.initials: Q
authors.lastname: Liu
authors.forename: Takumi
authors.initials: T
authors.lastname: Shimizu
authors.forename: Tamaki
authors.initials: T
authors.lastname: Uehara-Ichiki
authors.forename: Zujian
authors.initials: Z
authors.lastname: Wu
authors.forename: Lianhui
authors.initials: L
authors.lastname: Xie
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Omura
authors.forename: Taiyun
authors.initials: T
authors.lastname: Wei
mesh_terms: D000818:Animals; D001179:Arthropod Vectors / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D002460:Cell Line; D007313:Insecta; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity / Q000648:ultrastructure; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D017361:Viral Nonstructural Proteins