title: Thermoanaerobacter pentosaceus sp. nov., an anaerobic, extremely thermophilic, high ethanol-yielding bacterium isolated from household waste.
abstract: An extremely thermophilic, xylanolytic, spore-forming and strictly anaerobic bacterium, strain DTU01(T), was isolated from a continuously stirred tank reactor fed with xylose and household waste. Cells stained Gram-negative and were rod-shaped (0.5-2 µm in length). Spores were terminal with a diameter of approximately 0.5 µm. Optimal growth occurred at 70 °C and pH 7, with a maximum growth rate of 0.1 h(-1). DNA G+C content was 34.2 mol%. Strain DTU01(T) could ferment arabinose, cellobiose, fructose, galactose, glucose, lactose, mannitol, mannose, melibiose, pectin, starch, sucrose, xylan, yeast extract and xylose, but not cellulose, Avicel, inositol, inulin, glycerol, rhamnose, acetate, lactate, ethanol, butanol or peptone. Ethanol was the major fermentation product and a maximum yield of 1.39 mol ethanol per mol xylose was achieved when sulfite was added to the cultivation medium. Thiosulfate, but not sulfate, nitrate or nitrite, could be used as electron acceptor. On the basis of 16S rRNA gene sequence similarity, strain DTU01(T) was shown to be closely related to Thermoanaerobacter mathranii A3(T), Thermoanaerobacter italicus Ab9(T) and Thermoanaerobacter thermocopriae JT3-3(T), with 98-99 % similarity. Despite this, the physiological and phylogenetic differences (DNA G+C content, substrate utilization, electron acceptors, phylogenetic distance and isolation site) allow for the proposal of strain DTU01(T) as a representative of a novel species within the genus Thermoanaerobacter, for which the name Thermoanaerobacter pentosaceus sp. nov. is proposed, with the type strain DTU01(T) ( = DSM 25963(T) = KCTC 4529(T) = VKM B-2752(T) = CECT 8142(T)).
authors.affiliation: Department of Environmental Engineering, Technical University of Denmark, Miljoevej 113, 2800 Kongens Lyngby, Denmark.
authors.forename: Ana Faria
authors.initials: AF
authors.lastname: Tomás
authors.affiliation: Department of Environmental Engineering, Technical University of Denmark, Miljoevej 113, 2800 Kongens Lyngby, Denmark.
authors.forename: Dimitar
authors.initials: D
authors.lastname: Karakashev
authors.affiliation: Department of Environmental Engineering, Technical University of Denmark, Miljoevej 113, 2800 Kongens Lyngby, Denmark.
authors.forename: Irini
authors.initials: I
authors.lastname: Angelidaki
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005735:Garbage; D006358:Hot Temperature; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D046968:Thermoanaerobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014994:Xylose / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D000431:Ethanol; D014994:Xylose