title: Up-regulation of NICE-3 as a novel EDC gene could contribute to human hepatocellular carcinoma.
abstract: The epidermal differentiation complex (EDC) contains a large number of gene products which are crucial for the maturation of the human epidermis and can contribute to skin diseases, even carcinogenesis. It is generally acepted that activation of oncogenes and/or inactivation of tumor suppressor genes play pivotal roles in the process of carcinogenesis. Here, NICE-3, a novel EDC gene, was found to be up-regulated in human hepatocellular carcinoma (HCC) by quantitative real-time RT-PCR. Furthermore, overexpression of exogenous NICE-3 by recombinant plasmids could significantly promote cell proliferation, colony formation and soft agar colony formation in Focus and WRL-68 HCC cell lines. Reversely, NICE-3 silencing by RNA interference could markedly inhibit these malignant phenotypes in YY-8103 and MHCC-97H cells. Moreover, cell cycle analysis of MHCC-97H transfected with siRNA by flow cytometry showed that NICE-3 knockdown may inhibit cell growth via arrest in G0/G1 phase and hindering entry of cells into S phase. All data of our findings indicate that NICE-3 may contribute to human hepatocellular carcinoma by promoting cell proliferation.
authors.affiliation: Department of Hepatobiliary Surgery, Wuxi Municipal People's Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Yuan-Jiang
authors.initials: YJ
authors.lastname: Wei
authors.forename: Qin-Qin
authors.initials: QQ
authors.lastname: Hu
authors.forename: Cheng-Yu
authors.initials: CY
authors.lastname: Gu
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Wang
authors.forename: Ze-Guang
authors.initials: ZG
authors.lastname: Han
authors.forename: Bing
authors.initials: B
authors.lastname: Cai
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D059585:G1 Phase Cell Cycle Checkpoints; D015972:Gene Expression Regulation, Neoplastic; D055786:Gene Knockout Techniques; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D010957:Plasmids; D012334:RNA, Neoplasm / Q000235:genetics*; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection; D014410:Tumor Stem Cell Assay; D015854:Up-Regulation
chemical_list: D012334:RNA, Neoplasm; D034741:RNA, Small Interfering