title: Neuroprotective compounds from Salix pseudo-lasiogyne twigs and their anti-amnesic effects on scopolamine-induced memory deficit in mice.
abstract: Bioassay-guided fractionation of an 80% methanolic extract of Salix pseudo-lasiogyne twigs has resulted in the isolation of two new compounds (1-2) along with ten known ones (3-12). The new compounds were determined to be 3'-O-acetylsalicin (1) and 2',6'-O-acetylsalicortin (2) by using spectroscopic analyses. Compounds (3-12) were identified as salicin (3), 2'-O-acetylsalicin (4), salicortin (5), 2'-O-acetylsalicortin (6), 3'-O-acetylsalicortin (7), 6'-O-acetylsalicortin (8), 2'-O-(E)-ρ-coumaroylsalicortin (9), grandidentatin (10), isograndidentatin (11), and saligenin (12). Among the isolated compounds, compounds 2, 5, 6, 7, and 8 bearing 1-hydroxy-6-oxo-2-cyclohexenecarboxylate moiety significantly inhibited lipopolysaccharide-induced nitric oxide production in BV2 microglial cells in vitro. Further, we studied anti-amnesic activities of the 80% methanolic extract, the EtOAc fraction, and compound 6 from S. pseudo-lasiogyne. They exerted a significant cognitive-enhancing effect on scopolamine-induced memory deficit in mice. In addition, they also significantly increased the reduced activities of glutathione reductase and superoxide dismutase and the glutathione content in the hippocampus and cortex of scopolamine-induced amnesic mice.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Daehak-Dong, Gwanak-Gu, Seoul, Republic of Korea.
authors.forename: Heejung
authors.initials: H
authors.lastname: Yang
authors.forename: Sang Hoon
authors.initials: SH
authors.lastname: Lee
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Sung
authors.forename: Jinwoong
authors.initials: J
authors.lastname: Kim
authors.forename: Young Choong
authors.initials: YC
authors.lastname: Kim
mesh_terms: D000647:Amnesia / Q000139:chemically induced / Q000188:drug therapy; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001362:Avoidance Learning / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000201:enzymology*; D005978:Glutathione / Q000378:metabolism; D006624:Hippocampus / Q000201:enzymology*; D066298:In Vitro Techniques; D020128:Inhibitory Concentration 50; D008297:Male; D051379:Mice; D017628:Microglia / Q000187:drug effects; D015394:Molecular Structure; D018696:Neuroprotective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018697:Nootropic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry; D017207:Rats, Sprague-Dawley; D032108:Salix / Q000737:chemistry*; D012601:Scopolamine; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D018696:Neuroprotective Agents; D018697:Nootropic Agents; D010936:Plant Extracts; D012601:Scopolamine; D013482:Superoxide Dismutase; D005978:Glutathione