title: Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism.
abstract: Production of fuels and chemicals by industrial biotechnology requires efficient, safe and flexible cell factory platforms that can be used for production of a wide range of compounds. Here we developed a platform yeast cell factory for efficient provision of acetyl-CoA that serves as precursor metabolite for a wide range of industrially interesting products. We demonstrate that the platform cell factory can be used to improve the production of α-santalene, a plant sesquiterpene that can be used as a perfume by four-fold. This strain would be a useful tool to produce a wide range of acetyl-CoA-derived products.
authors.affiliation: Department of Chemical and Biological Engineering, Chalmers University of Technology, SE-41296 Gothenburg, Sweden.
authors.forename: Yun
authors.initials: Y
authors.lastname: Chen
authors.forename: Laurent
authors.initials: L
authors.lastname: Daviet
authors.forename: Michel
authors.initials: M
authors.lastname: Schalk
authors.forename: Verena
authors.initials: V
authors.lastname: Siewers
authors.forename: Jens
authors.initials: J
authors.lastname: Nielsen
mesh_terms: D000105:Acetyl Coenzyme A / Q000235:genetics* / Q000378:metabolism*; D061249:Batch Cell Culture Techniques / Q000379:methods*; D024861:Genetic Enhancement / Q000379:methods*; D015202:Protein Engineering / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology*; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D012717:Sesquiterpenes; D000105:Acetyl Coenzyme A