title: [Subtype analysis of gp41 gene of HIV-1 among men who have sex with men in Zhengzhou].
abstract: OBJECTIVE
  This study aimed to investigate the subtype distribution of gp41 gene of human immunodeficiency virus-1 (HIV-1) among men who have sex with men (MSM) in Zhengzhou.
  
  
  METHODS
  Thirty blood samples were collected from men who have sex with men infected by HIV. The complete gp41 gene was amplified by RT-PCR and nested-PCR and sequenced. All sequences were edited by Bioedit and subtyped with HIV sequence library US Los Alamos National Laboratory and online genotyping software provided by American National Center of Biotechnology Information. Phylogenetic analysis of gp41 gene was performed using the MEGA 3.1 software, and the genic dispersion rates among subtype of gp41 gene were analyzed.
  
  
  RESULTS
  A total of eighteen gene sequences of HIV-1 gp41 gene were obtained from thirty men who have sex with men infected by HIV, which belonged to subtype CRF15-01B (50% (9/18)), CRF01-AE (22% (4/18)), CRF07-B (22% (4/18)) and B (6% (1/18)), respectively. The intersubtype HIV-1 strains aggregate with according reference strains. The genetic distance inter-subtype of subtype CRF15-01B, CRF01-AE and CRF07-B were 0.050 ± 0.007, 0.052 ± 0.009 and 0.082 ± 0.012, respectively.
  
  
  CONCLUSIONS
  The prevalent subtypes of HIV-1 among among MSM in Zhengzhou was complicated and recombinant HIV-1 strains were the most prevalent strains.
authors.affiliation: Zhengzhou University People's Hospital, College of Basic Medical Sciences of Zhengzhou University, Zhengzhou 450003, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Song
authors.forename: Guo-qing
authors.initials: GQ
authors.lastname: Sun
authors.forename: Yan-min
authors.initials: YM
authors.lastname: Zhang
authors.forename: Wei-guo
authors.initials: WG
authors.lastname: Cui
authors.forename: Hui-juan
authors.initials: HJ
authors.lastname: An
authors.forename: Yu-hui
authors.initials: YH
authors.lastname: An
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.forename: Yu-zhou
authors.initials: YZ
authors.lastname: Bao
mesh_terms: D005838:Genotype; D015700:HIV Envelope Protein gp41 / Q000235:genetics*; D015658:HIV Infections / Q000453:epidemiology* / Q000821:virology*; D015497:HIV-1 / Q000235:genetics*; D018451:Homosexuality, Male; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D015700:HIV Envelope Protein gp41; C514787:gp41 protein, Human immunodeficiency virus 1