title: The role of bacterial membrane proteins in the internalization of microcin MccJ25 and MccB17.
abstract: Microcins are gene-encoded antibacterial peptides of low molecular mass (<10 kDa), produced by Enterobactericeae. They are produced and secreted under conditions of limited essential nutrients and are active against related species. Bacterial strains under starvation conditions can produce and release microcins that can kill microcin-sensitive cells and therefore have more nutrients for survival. The outer-membrane protein OmpF and FhuA TonB-dependent pathways facilitate the internalization of the MccB17 and MccJ25 microcins into the target cell respectively. The inner-membrane protein SbmA transports the microcins through the inner membrane to the cytoplasmic face. Inside the cell, MccB17 targets DNA gyrase, whereas MccJ25 inhibits the bacterial RNA polymerase.
authors.affiliation: Division of Molecular Biosciences, Imperial College London, Exhibition Road, South Kensington, London SW7 2AZ, UK.
authors.forename: Indran
authors.initials: I
authors.lastname: Mathavan
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Beis
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000502:physiology*; D001430:Bacteriocins / Q000737:chemistry / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000502:physiology*; D026901:Membrane Transport Proteins / Q000502:physiology; D008958:Models, Molecular; D018272:Porins / Q000737:chemistry / Q000502:physiology*; D011485:Protein Binding; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D021381:Protein Transport
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001430:Bacteriocins; D029968:Escherichia coli Proteins; C050900:FhuA protein, E coli; D026901:Membrane Transport Proteins; C109237:OmpF protein; D018272:Porins; C529573:SbmA protein, E coli; C020411:microcin