title: Mutant p53 in cell adhesion and motility.
abstract: Pro-oncogenic properties of mutant p53 were investigated with the aid of migration assays, adhesion assays, and soft agar growth assays using cells stably expressing gain-of-function p53 mutants. To determine cell migration, "wound-healing" (scratch) assays and haptotactic (chamber) assays were used. H1299 cells expressing mutant p53 were found to migrate more rapidly than cells transfected with empty vector alone. Results from both types of migration assay were broadly similar. Migratory ability differed for different p53 mutants, suggesting allele-specific effects. Cells expressing p53 mutants also showed enhanced adhesion to extracellular matrix compare to controls. Furthermore, stable transfection of mutant p53-H179L into NIH3T3 fibroblasts was sufficient to allow anchorage-independent growth in soft agar.
authors.affiliation: Philips Institute of Oral & Craniofacial Molecular Biology, School of Dentistry, Virginia Commonwealth University, Richmond, VA, USA.
authors.forename: W Andrew
authors.initials: WA
authors.lastname: Yeudall
authors.forename: Katharine H
authors.initials: KH
authors.lastname: Wrighton
authors.forename: Sumitra
authors.initials: S
authors.lastname: Deb
mesh_terms: D000483:Alleles; D000818:Animals; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D005347:Fibroblasts / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D051379:Mice; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D041681:NIH 3T3 Cells; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D058106:Wound Closure Techniques
chemical_list: D050505:Mutant Proteins; D016159:Tumor Suppressor Protein p53