title: Rigid 5'-6-locked phenanthroline-derived nucleosides chelated to ruthenium and europium ions.
abstract: We describe complexes of ruthenium and europium with rigid, 5'-6-locked 1,10-phenanthroline-containing nucleosides. Both nucleosides were synthesized from condensation of 5-amino-2'-deoxycytidine with the corresponding diketone. The ruthenium nucleoside displayed fluorescence characteristic of polypyridine ruthenium complexes with a maximum at 616 nm and a quantum yield of 0.011. Binding of europium to the 1,10-phenanthroline-2,9-diacid moiety of the lanthanide binding nucleoside showed formation of a 1:1 complex with emission at 570-630 nm, whose emission was enhanced by addition of two phenanthroline ligands. The lanthanide-binding nucleoside was incorporated into DNA oligonucleotides and shown to selectively bind one equivalent of europium ions.
authors.affiliation: University of Iceland, Department of Chemistry, Science Institute, Dunhaga 3, 107 Reykjavik, Iceland.
authors.forename: Kristmann
authors.initials: K
authors.lastname: Gislason
authors.forename: Snorri Th
authors.initials: ST
authors.lastname: Sigurdsson
mesh_terms: D002614:Chelating Agents / Q000737:chemistry; D056831:Coordination Complexes / Q000737:chemistry; D005063:Europium / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D009705:Nucleosides / Q000737:chemistry*; D010618:Phenanthrolines / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D002614:Chelating Agents; D056831:Coordination Complexes; D007477:Ions; D009705:Nucleosides; D010618:Phenanthrolines; D005063:Europium; D012428:Ruthenium