title: [Recent advances in quantitative proteomics as a sensitive tool to quantify             drug transporters and drug metabolizing enzymes at the human blood-brain             barrier].
abstract: Since its discovery at the beginning of the 20th century, the blood-brain barrier (BBB)           has been considered for a long time as a "physical barrier" able to limit brain           distribution of highly molecular weight and/or polar compounds. This early concept of an           anatomical barrier between the blood and the brain was supported by the finding of unique           tight junctions between the brain endothelial cells so that they formed a continuous wall           preventing the paracellular diffusion of solutes. In the middle of the 50's, BBB has been           proposed as a "biochemical barrier" able to control the supply of brain to essential           nutriments. More recently, BBB was evidenced as a key element in controlling effects of           central nervous system drugs, since it plays a critical role in the uptake and efflux of           drugs from the blood to the brain, or vice versa, hence affecting their           concentrations and effects in the central nervous system (CNS). The BBB has therefore been           more recently defined as a "pharmacological barrier" since the endothelial cells were           found to contain a range of metabolizing enzymes and transporters that control the rate           and extent of drugs reaching the brain parenchyma via transcellular           pathway. The emergence of new quantitative proteomic approaches allows quantifying these           transporters and enzymes at the BBB, opening the way to identify new drugs that may be           targeted to the brain.
authors.affiliation: Universite Paris Descartes, Paris, France.
authors.forename: Aude
authors.initials: A
authors.lastname: Jacob
authors.forename: Xavier
authors.initials: X
authors.lastname: Declèves
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Scherrmann
mesh_terms: D001812:Blood-Brain Barrier / Q000737:chemistry* / Q000201:enzymology / Q000502:physiology; D001921:Brain / Q000378:metabolism; D002491:Central Nervous System Agents / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D042783:Endothelial Cells / Q000737:chemistry* / Q000201:enzymology; D006801:Humans; D026901:Membrane Transport Proteins / Q000032:analysis*; D040901:Proteomics / Q000639:trends*
chemical_list: D002491:Central Nervous System Agents; D026901:Membrane Transport Proteins; D003577:Cytochrome P-450 Enzyme System