title: Single-walled carbon nanotubes alter cytochrome c electron transfer and modulate mitochondrial function.
abstract: Single-walled carbon nanotubes (SWCNTs) are broadly used for various biomedical applications such as drug delivery, in vivo imaging, and cancer photothermal therapy due to their unique physiochemical properties. However, once they enter the cells, the effects of SWCNTs on the intracellular organelles and macromolecules are not comprehensively understood. Cytochrome c (Cyt c), as a key component of the electron transport chain in mitochondria, plays an essential role in cellular energy consumption, growth, and differentiation. In this study, we found the mitochondrial membrane potential and mitochondrial oxygen uptake were greatly decreased in human epithelial KB cells treated with SWCNTs, which accompanies the reduction of Cyt c. SWCNTs deoxidized Cyt c in a pH-dependent manner, as evidenced by the appearance of a 550 nm characteristic absorption peak, the intensity of which increased as the pH increased. Circular dichroism measurement confirmed the pH-dependent conformational change, which facilitated closer association of SWCNTs with the heme pocket of Cyt c and thus expedited the reduction of Cyt c. The electron transfer of Cyt c is also disturbed by SWCNTs, as measured with electron spin resonance spectroscopy. In conclusion, the redox activity of Cyt c was affected by SWCNTs treatment due to attenuated electron transfer and conformational change of Cyt c, which consequently changed mitochondrial respiration of SWCNTs-treated cells. This work is significant to SWCNTs research because it provides a novel understanding of SWCNTs' disruption of mitochondria function and has important implications for biomedical applications of SWCNTs.
authors.affiliation: Laboratory of Nanomedicine and Nanosafety, Division of Nanomedicine and Nanobiology, National Center for Nanoscience and Technology, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Ma
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Zhang
authors.forename: Li-Rong
authors.initials: LR
authors.lastname: Wang
authors.forename: Xue
authors.initials: X
authors.lastname: Xue
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Sun
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.forename: Guozhang
authors.initials: G
authors.lastname: Zou
authors.forename: Xia
authors.initials: X
authors.lastname: Wu
authors.forename: Paul C
authors.initials: PC
authors.lastname: Wang
authors.forename: Wayne G
authors.initials: WG
authors.lastname: Wamer
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Yin
authors.forename: Kaiyuan
authors.initials: K
authors.lastname: Zheng
authors.forename: Xing-Jie
authors.initials: XJ
authors.lastname: Liang
mesh_terms: D001692:Biological Transport / Q000187:drug effects; D019069:Cell Respiration / Q000187:drug effects; D045304:Cytochromes c / Q000378:metabolism*; D004579:Electron Transport / Q000187:drug effects; D006801:Humans; D007624:KB Cells; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism*; D037742:Nanotubes, Carbon / Q000009:adverse effects* / Q000737:chemistry*; D010100:Oxygen / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D037742:Nanotubes, Carbon; D017382:Reactive Oxygen Species; D045304:Cytochromes c; D010100:Oxygen