title: Phellinus baumii extract influences pathogenesis of Brucella abortus in phagocyte by disrupting the phagocytic and intracellular trafficking pathway.
abstract: OBJECTIVE
  To clarify the effects of Phellinus baumii ethanol extract (PBE) on Brucella abortus pathogenesis in phagocytes focusing on the phagocytic and intracellular trafficking pathway.
  
  
  RESULTS
  The effects of PBE on Br. abortus infection in macrophages were evaluated through an adherence and infection assays and an analysis of LAMP-1 staining. The phosphorylation of ERK1/2 and the F-actin polymerization associated with PBE during Br. abortus uptake were detected by immunoblotting and FACS, respectively. The survival of Br. abortus in pure culture was remarkably reduced by PBE in a dose-dependent manner. PBE-treated cells showed significantly decreased uptake, intracellular replication and adherence of Br. abortus. The declines of ERK1/2 phosphorylation and F-actin polymerization following Br. abortus entry were apparent in PBE-treated cells compared with the control. Moreover, the co-localization of Br. abortus-containing phagosomes with LAMP-1 was elevated in PBE-treated cells compared with the control during intracellular trafficking.
  
  
  CONCLUSIONS
  Phellinus baumii ethanol extract may possess the modulatory effect on pathogenesis of Br. abortus through disrupting the phagocytic and intracellular trafficking pathway in phagocyte.
  
  
  CONCLUSIONS
  The potential modulation of PBE to Br. abortus pathogenesis could provide an alternative approach to control of brucellosis, contributing to attenuate Br. abortus manifestation in hosts.
authors.affiliation: Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Korea.
authors.forename: J J
authors.initials: JJ
authors.lastname: Lee
authors.forename: D H
authors.initials: DH
authors.lastname: Kim
authors.forename: D G
authors.initials: DG
authors.lastname: Kim
authors.forename: H J
authors.initials: HJ
authors.lastname: Lee
authors.forename: W
authors.initials: W
authors.lastname: Min
authors.forename: M H
authors.initials: MH
authors.lastname: Rhee
authors.forename: B S
authors.initials: BS
authors.lastname: Yun
authors.forename: S
authors.initials: S
authors.lastname: Kim
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001487:Basidiomycota; D001692:Biological Transport / Q000187:drug effects; D002003:Brucella abortus / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival; D000431:Ethanol; D008264:Macrophages / Q000166:cytology / Q000382:microbiology*; D051379:Mice; D050296:Microbial Viability; D010587:Phagocytosis / Q000187:drug effects
chemical_list: D000199:Actins; D000900:Anti-Bacterial Agents; D000431:Ethanol