title: Preparation of an immunoafﬁnity column for the clean-up of fermented food samples contaminated with citrinin.
abstract: An immunoafﬁnity column (IAC) for the clean-up of citrinin-contaminated food samples was prepared by using silica gel immobilised with a anti-citrinin antibody. Antibodies produced against citrinin-bovine serum albumin (BSA) were covalently immobilised onto a silica-based solid support to prepare the IAC. The selective extraction and clean-up ability of the IAC was evaluated by capillary zone electrophoresis coupled with ultraviolet detection. Clean-up conditions such as eluting solutions kind, concentrations, pH and volumes were optimised for citrinin by using the IAC. IACs were applied to citrinin-contaminated foods such as red yeast rice and monascus colour. The method's performance was acceptable in terms of recoveries, which ranged from 80.4% to 97.1% for citrinin-spiked samples at levels of 50, 100 and 200 µg kg(-1), and the relative standard deviation ranged from 5.3% to 10.5%.
authors.affiliation: College of Life Science, Shandong Normal University, Jinan, P R China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhu
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Bing
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized; D001688:Biological Products / Q000737:chemistry; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods*; D002953:Citrinin / Q000032:analysis* / Q000276:immunology; D019587:Dietary Supplements / Q000032:analysis; D005285:Fermentation; D005506:Food Contamination / Q000032:analysis*; D032741:Monascus / Q000737:chemistry; D012710:Serum Albumin, Bovine
chemical_list: D055910:Antibodies, Immobilized; D001688:Biological Products; C117343:red yeast rice; D012710:Serum Albumin, Bovine; D002953:Citrinin