title: Enrichment of outer membrane vesicles shed by Legionella pneumophila.
abstract: The production of outer membrane vesicles (OMVs) is a widespread phenomenon employed by bacteria to secrete cell envelope components into the environment. A contribution of Legionella pneumophila OMVs to the pathogenesis of Legionnaires' disease is likely due to the high number of virulence-related proteins in the vesicles. OMVs are isolated from the supernatant of liquid cultures of L. pneumophila. After low-speed centrifugation, residual bacteria and cell fragments are eliminated by passing the supernatant through a filter. OMVs are pelleted by ultracentrifugation and resuspended in buffer. The isolated OMVs can be analyzed for their molecular components and their interactions with host structures, bacterial cells, or surfaces.
authors.affiliation: Institut für Mikrobiologie, Technische Universität Braunschweig, Braunschweig, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Jäger
authors.forename: Michael
authors.initials: M
authors.lastname: Steinert
mesh_terms: D055252:Cell-Derived Microparticles / Q000378:metabolism*; D055354:Exosomes / Q000378:metabolism*; D016952:Legionella pneumophila / Q000254:growth & development / Q000378:metabolism*