title: Layer-by-layer assembled polypeptide capsules for platinum-based pro-drug delivery.
abstract: Platinum(IV), a pro-drug of platinum(II), was conjugated to poly(l-lysine) (PLL), and then assembled with poly(glutamic acid) (PGA) through a layer-by-layer (LbL) approach on colloidal silica templates. After removal of the templates, biodegradable PGA/PLL-Pt(IV) multilayer capsules (diameter = 0.5 μm) with 10 μg of platinum incorporated into each bilayer were obtained. Under acidic and/or reductive conditions, the amount and rate of platinum released from the capsules were increased, which are desirable traits for platinum-based anticancer drug delivery systems. Furthermore, in vitro evaluation showed that the PGA/PLL-Pt(IV) multilayer microcapsules displayed higher cytotoxicity (IC(50Pt) = 3.5 μg/mL) against colon cancer cells CT-26 than that of free cisplatin (IC(50Pt) = 8.6 μg/mL). This enhanced cytotoxicity was attributed to the effective internalization of the capsules by the cancer cells, which was observed by confocal laser scanning microscopy (CLSM) imaging.
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Graduate School of Chinese Academy of Sciences, Renmin Str. 5625, Changchun 130022, PR China.
authors.forename: Dongfang
authors.initials: D
authors.lastname: Zhou
authors.forename: Haihua
authors.initials: H
authors.lastname: Xiao
authors.forename: Fanbo
authors.initials: F
authors.lastname: Meng
authors.forename: Shaoyang
authors.initials: S
authors.lastname: Zhou
authors.forename: Jinshan
authors.initials: J
authors.lastname: Guo
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Li
authors.forename: Xiabin
authors.initials: X
authors.lastname: Jing
authors.forename: Yubin
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology; D003102:Colloids; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000494:pharmacology; D004337:Drug Carriers / Q000138:chemical synthesis*; D004705:Endocytosis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D018613:Microscopy, Confocal; D011099:Polyglutamic Acid / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry; D011355:Prodrugs / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D003102:Colloids; D056831:Coordination Complexes; D004337:Drug Carriers; D011355:Prodrugs; D011107:Polylysine; D011099:Polyglutamic Acid; D012822:Silicon Dioxide; D002945:Cisplatin