title: Safety assessment of ovarian cryopreservation and transplantation in nude mice bearing human epithelial ovarian cancer.
abstract: OBJECTIVE
  Nude mice with orthotopic transplantation of human ovarian epithelial cancer were used to investigate screening criteria for paraneoplastic normal ovarian tissue and the security of the freezing and thawing for ovarian tissue transplantation.
  
  
  METHODS
  Expression of CK-7, CA125, P53, survivin, MMP-2/TIMP- 2 in paraneoplastic normal ovarian tissues were detected by RT-PCR as well as immunohistochemistry. The tissues of the groups with all negative indicators of RT-PCR, all negative indicators of immunohistochemistry, negative expression of CK-7, CA125 and survivin, positive expression of CK-7, CA125 and survivin, cancer tissues and normal ovarian tissues of nude mice were used for freezing and thawing transplantation, to analyze overt and occult carcinogenesis rates after transplantation.
  
  
  RESULTS
  When all indicators or the main indicators, CK-7, CA125 and survivin, were negative, tumorigenesis did not occur after transplantation. In addition the occult carcinogenesis rate was lower than in the group with positive expression of CK-7, CA125 and survivin (P<0.01). After subcutaneous and orthotopic transplantation of ovarian tissues, rates did not change (P>0.05). There was no statistical significance among rates after transplantation of ovarian tissues which were obtained under different severity conditions (P>0.05).
  
  
  CONCLUSIONS
  Negative expression of CK-7, CA125 and survivin can be treated as screening criteria for security of ovarian tissues for transplantation. Immunohistochemical methods can be used as the primary detection approach. Both subcutaneous and orthotopic transplantation are safe. The initial severity does not affect the carcinogenesis rate after tissue transplantation. Freezing and thawing ovarian tissue transplantation in nude mice with human epithelial ovarian carcinoma is feasible and safe.
authors.affiliation: Department of Gynecology, Hainan Provincial People's Hospital, Hainan, China. genhaizhu@163.com
authors.forename: Gen-Hai
authors.initials: GH
authors.lastname: Zhu
authors.forename: Sheng-Tan
authors.initials: ST
authors.lastname: Wang
authors.forename: Zhao-Xin
authors.initials: ZX
authors.lastname: Yang
authors.forename: Jun-Hong
authors.initials: JH
authors.lastname: Cai
authors.forename: Chun-Ying
authors.initials: CY
authors.lastname: Chen
authors.forename: Mao-Zhong
authors.initials: MZ
authors.lastname: Yao
authors.forename: Lan
authors.initials: L
authors.lastname: Hong
authors.forename: Guo-Li
authors.initials: GL
authors.lastname: He
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Yang
mesh_terms: D000818:Animals; D018394:CA-125 Antigen / Q000235:genetics / Q000378:metabolism; D000077216:Carcinoma, Ovarian Epithelial; D015925:Cryopreservation; D005260:Female; D059247:Fertility Preservation; D006801:Humans; D007150:Immunohistochemistry; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism; D053552:Keratin-7 / Q000235:genetics / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009375:Neoplasms, Glandular and Epithelial / Q000175:diagnosis* / Q000209:etiology / Q000601:surgery; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000209:etiology / Q000601:surgery; D010053:Ovary / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D012092:Replantation / Q000009:adverse effects*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077022:Survivin; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: C107489:BIRC5 protein, human; D018394:CA-125 Antigen; D051034:Inhibitor of Apoptosis Proteins; D053552:Keratin-7; D000077022:Survivin; D016159:Tumor Suppressor Protein p53; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2