title: Acclimation of leaves to low light produces large grana: the origin of the predominant attractive force at work.
abstract: Photosynthetic membrane sacs (thylakoids) of plants form granal stacks interconnected by non-stacked thylakoids, thereby being able to fine-tune (i) photosynthesis, (ii) photoprotection and (iii) acclimation to the environment. Growth in low light leads to the formation of large grana, which sometimes contain as many as 160 thylakoids. The net surface charge of thylakoid membranes is negative, even in low-light-grown plants; so an attractive force is required to overcome the electrostatic repulsion. The theoretical van der Waals attraction is, however, at least 20-fold too small to play the role. We determined the enthalpy change, in the spontaneous stacking of previously unstacked thylakoids in the dark on addition of Mg(2+), to be zero or marginally positive (endothermic). The Gibbs free-energy change for the spontaneous process is necessarily negative, a requirement that can be met only by an increase in entropy for an endothermic process. We conclude that the dominant attractive force in thylakoid stacking is entropy-driven. Several mechanisms for increasing entropy upon stacking of thylakoid membranes in the dark, particularly in low-light plants, are discussed. In the light, which drives the chloroplast far away from equilibrium, granal stacking accelerates non-cyclic photophosphorylation, possibly enhancing the rate at which entropy is produced.
authors.affiliation: Division of Plant Science, Research School of Biology, The Australian National University, Canberra, Australian Capital Territory 0200, Australia.
authors.forename: Husen
authors.initials: H
authors.lastname: Jia
authors.forename: John R
authors.initials: JR
authors.lastname: Liggins
authors.forename: Wah Soon
authors.initials: WS
authors.lastname: Chow
mesh_terms: D000064:Acclimatization; D000255:Adenosine Triphosphate / Q000378:metabolism; D031055:Alocasia / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D025222:Chloroplast Proton-Translocating ATPases / Q000378:metabolism; D003624:Darkness; D004735:Energy Transfer; D019277:Entropy; D008027:Light; D045342:Light-Harvesting Protein Complexes / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D015636:Magnesium Chloride / Q000494:pharmacology; D053141:Organelle Size / Q000528:radiation effects*; D010785:Photophosphorylation; D010788:Photosynthesis; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D055672:Static Electricity; D020524:Thylakoids / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*
chemical_list: D045342:Light-Harvesting Protein Complexes; D015636:Magnesium Chloride; D000255:Adenosine Triphosphate; D025222:Chloroplast Proton-Translocating ATPases; D008274:Magnesium