title: Development of super-dense transfected cell microarrays generated by piezoelectric inkjet printing.
abstract: Super-dense transfected cell microarrays (TCMs) were created by a piezoelectric inkjet printer on a glass substrate that had been grafted with poly(ethylene glycol) (PEG). The micro-spots that contained plasmid and extra-cellular matrix (ECM) protein were separated from one another by a hydrophilic barrier generated by PEG. We successfully constructed the densest TCMs with spots of 50 μm in diameter and 150 μm in pitch.
authors.affiliation: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. s-fujita@aist.go.jp
authors.forename: Satoshi
authors.initials: S
authors.lastname: Fujita
authors.forename: Reiko
authors.initials: R
authors.lastname: Onuki-Nagasaki
authors.forename: Junji
authors.initials: J
authors.lastname: Fukuda
authors.forename: Junko
authors.initials: J
authors.lastname: Enomoto
authors.forename: Suichi
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Masato
authors.initials: M
authors.lastname: Miyake
mesh_terms: D062028:Bioprinting / Q000379:methods*; D005353:Fibronectins / Q000737:chemistry / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D011092:Polyethylene Glycols / Q000737:chemistry; D046888:Tissue Array Analysis / Q000295:instrumentation* / Q000379:methods*; D014162:Transfection / Q000295:instrumentation* / Q000379:methods*
chemical_list: D005353:Fibronectins; D011092:Polyethylene Glycols