title: UHPLC/ESI-Q-TOF-MS method for the measurement of dopamine in rodent striatal tissue: a comparative effects of intranasal administration of ropinirole solution over nanoemulsion.
abstract: An ultra high performance liquid chromatography-electrospray ionization-tandem mass spectrometric method (UHPLC/ESI-Q-TOF-MS) for the analysis of dopamine (DA) in Wistar rat brain homogenate has been developed and validated. The chromatographic separation was achieved on a Waters ACQUITY UPLC™ BEH C18 (100.0 mm × 2.1 mm; 1.7 µm) column using isocratic mobile phase, consisting of acetonitrile and Formic acid (0.1% w/v) (10: 90; v/v), at a flow rate of 0.15 ml min(-1) . The transitions occurred at m/z 154.04 → 137.006 for DA, and m/z 184.204 → 166.08 for the internal standard. The recovery of the analytes from Wistar rat brain homogenate was optimized using liquid-liquid extraction technique (LLE) in ethyl acetate. The total run time was 3.5 min and the elution of DA occurred at 1.44 ± 0.05 min. The linear dynamic range was established over the concentration range 75-750 ng mL(-1) (r(2) ; 0.9921 ± 0.0005) for DA. The intra-assay and inter-assay accuracy in terms of % CV was in the range 0.73-2.80. The lower limit of detection (LOD) and quantitation (LOQ) for DA was 0.278 and 0.844 ng mL(-1) , respectively. Analytes were stable under various conditions (in autosampler, during freeze-thaw, at room temperature, and under deep-freeze conditions). The developed method was successfully applied for in vivo profiling in rodents.
authors.affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University, New Delhi-, 110062, India.
authors.forename: Gulam
authors.initials: G
authors.lastname: Mustafa
authors.forename: Niyaz
authors.initials: N
authors.lastname: Ahmad
authors.forename: Sanjula
authors.initials: S
authors.lastname: Baboota
authors.forename: Javed
authors.initials: J
authors.lastname: Ali
authors.forename: Alka
authors.initials: A
authors.lastname: Ahuja
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004298:Dopamine / Q000032:analysis*; D015259:Dopamine Agents / Q000032:analysis*; D018491:Dopamine Agonists / Q000008:administration & dosage*; D007211:Indoles / Q000008:administration & dosage*; D057230:Limit of Detection; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D015259:Dopamine Agents; D018491:Dopamine Agonists; D007211:Indoles; C046649:ropinirole; D004298:Dopamine