title: Mycobacterium parakoreense sp. nov., a slowly growing non-chromogenic species related to Mycobacterium koreense, isolated from a human clinical specimen.
abstract: A previously undescribed, slowly growing, non-chromogenic Mycobacterium strain (299(T)) was isolated from the sputum sample of a patient with a symptomatic pulmonary infection. Phenotypically, strain 299(T) was generally similar to Mycobacterium koreense DSM 45576(T) and Mycobacterium triviale ATCC 23292(T). The 16S rRNA gene sequence of strain 299(T) was similar to that of M. koreense DSM 45576(T) (GenBank accession no. AY734996, 99.5% similarity); however, it differed substantially from that of M. triviale ATCC 23292(T) (X88924, 98.2%). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 299(T) clustered together with M. koreense DSM 45576(T) and M. triviale ATCC 23292(T), supported by high bootstrapping values (99%). Unique mycolic acid profiles and phylogenetic analysis based on two different chronometer molecules, the hsp65 and rpoB genes, strongly supported the taxonomic status of this strain as representing a distinct species. These data support the conclusion that strain 299(T) represents a novel mycobacterial species, for which the name Mycobacterium parakoreense sp. nov. is proposed. The type strain is 299(T) (=DSM 45575(T)=KCTC 19818(T)).
authors.affiliation: Department of Microbiology and Immunology, Biomedical Sciences, Liver Research Institute, Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
authors.forename: Byoung-Jun
authors.initials: BJ
authors.lastname: Kim
authors.affiliation: Department of Microbiology and Immunology, Biomedical Sciences, Liver Research Institute, Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
authors.forename: Seok-Hyun
authors.initials: SH
authors.lastname: Hong
authors.affiliation: The Korean Institute of Tuberculosis, The Korean National Tuberculosis Association, Seoul 137-140, Republic of Korea.
authors.forename: Hee-Kyung
authors.initials: HK
authors.lastname: Yu
authors.affiliation: The Korean Institute of Tuberculosis, The Korean National Tuberculosis Association, Seoul 137-140, Republic of Korea.
authors.forename: Young-Gil
authors.initials: YG
authors.lastname: Park
authors.affiliation: Department of Laboratory Medicine, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan 682-714, Republic of Korea.
authors.forename: Joseph
authors.initials: J
authors.lastname: Jeong
authors.affiliation: Department of Laboratory Medicine, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan 682-714, Republic of Korea.
authors.forename: Seon Ho
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan 682-714, Republic of Korea.
authors.forename: Sung-Ryul
authors.initials: SR
authors.lastname: Kim
authors.affiliation: Department of Microbiology, College of Medicine, Chung-Ang University, Seoul, Republic of Korea.
authors.forename: Kijeong
authors.initials: K
authors.lastname: Kim
authors.affiliation: Department of Microbiology and Immunology, Biomedical Sciences, Liver Research Institute, Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
authors.forename: Yoon-Hoh
authors.initials: YH
authors.lastname: Kook
authors.affiliation: Department of Microbiology and Immunology, Biomedical Sciences, Liver Research Institute, Institute of Endemic Diseases, Seoul National University Medical Research Center (SNUMRC), Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.
authors.forename: Bum-Joon
authors.initials: BJ
authors.lastname: Kim
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009164:Mycobacterium Infections / Q000382:microbiology; D009171:Mycolic Acids / Q000032:analysis; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013183:Sputum / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D009171:Mycolic Acids; D012336:RNA, Ribosomal, 16S