title: Recent advances in laser tweezers Raman spectroscopy (LTRS) for label-free analysis of single cells.
abstract: Laser tweezers Raman spectroscopy (LTRS), a technique that integrates optical tweezers with confocal Raman spectroscopy, is a variation of micro-Raman spectroscopy that enables the manipulation and biochemical analysis of single biological particles in suspension. This article provides an overview of the LTRS method, with an emphasis on highlighting recent advances over the past several years in the development of the technology and several new biological and biomedical applications that have been demonstrated. A perspective on the future developments of this powerful cytometric technology will also be presented.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of California, Davis, Sacramento, CA 95817, USA. jwjchan@ucdavis.edu
authors.forename: James W
authors.initials: JW
authors.lastname: Chan
mesh_terms: D001671:Biochemistry / Q000379:methods; D003584:Cytological Techniques / Q000379:methods; D004867:Equipment Design; D004907:Erythrocyte Deformability; D004912:Erythrocytes / Q000166:cytology; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D044085:Microfluidics; D008856:Microscopy, Fluorescence / Q000379:methods; D009369:Neoplasms / Q000378:metabolism; D052898:Optical Tweezers; D055095:Optics and Photonics; D010100:Oxygen / Q000378:metabolism; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013171:Spores, Bacterial / Q000378:metabolism; D013997:Time Factors
chemical_list: D010100:Oxygen