title: HPLC analysis of stemokerrine and oxystemokerrine in the Thai medicinal plant Stemona kerrii.
abstract: A HPLC-UV method was developed and validated for determination of stemokerrine and oxystemokerrine in Stemona kerrii roots. The chromatographic separation was performed on a Hypersil BDS Cl8-column eluted with methanol: 50 mM ammonium acetate solution using a gradient system with a flow rate of 1 mL/min and detection at 300 nm. Stemokerrine and oxystemokerrine showed a linear relationship within the range of 0.5-100 microg/mL. The method was shown to be precise with a RSD <2%. The average percent recovery of stemokerrine was 101.6% and for oxystemokerrine 99.5%. Two samples of S. kerrii were analyzed and the average contents of stemokerrine and oxystemokerrine were 0.2 and 0.05%, w/w, respectively. The present work will provide a useful standardization method for S. kerrii raw materials for further pharmaceutical development.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Sumet
authors.initials: S
authors.lastname: Kongkiatpaiboon
authors.forename: Vichien
authors.initials: V
authors.lastname: Keeratinijakal
authors.forename: Wandee
authors.initials: W
authors.lastname: Gritsanapan
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006575:Heterocyclic Compounds, 3-Ring / Q000032:analysis*; D007202:Indicators and Reagents; D007783:Lactones / Q000032:analysis*; D057230:Limit of Detection; D018517:Plant Roots / Q000737:chemistry; D012044:Regression Analysis; D015203:Reproducibility of Results; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D041901:Stemonaceae / Q000737:chemistry*; D013785:Thailand
chemical_list: D006575:Heterocyclic Compounds, 3-Ring; D007202:Indicators and Reagents; D007783:Lactones; D012997:Solvents; C576773:oxystemokerrine; C576772:stemokerrine