title: Effects of cell salvage on erythrocyte 2,3-disphosphoglycerate and G-6-PD levels and phosphatidylserine expression.
abstract: BACKGROUND
  The aim of this study was to investigate the contribution of the perioperative cell salvage process to the changes in erythrocyte 2,3-disphosphoglycerate (2,3-DPG) and glucose-6-phosphate dehydrogenase (G-6-PD) levels and phosphatidylserine (PS) expression using a self-control study comparing washed blood with venous blood.
  
  
  METHODS
  Thirty patients undergoing elective heart and blood vessel surgery were enrolled. Blood was collected and processed using a Fresenius continuous autotransfusion system device. 2,3-DPG and G-6-PD activities, as well as PS expression, from both venous and washed red blood cells (RBC) were measured. We also compared these indicators among washed RBC at 6 h, washed RBC at 0 h, and venous RBC.
  
  
  RESULTS
  2,3-DPG and G-6-PD activities in washed blood at 0 h were significantly higher than in venous RBC (2,3-DPG, venous vs. washed blood P < 0.05; G-6-PD, venous vs. washed blood P < 0.05). Flow cytometry results showed no differences between venous blood and washed RBC at 0 h (n = 23, P > 0.05). 2,3-DPG activity in washed RBC that were allowed to stand for 6 h decreased compared with that in venous blood (venous vs. washed blood at 6 h P < 0.01), whereas no significant difference was observed in G-6-PD activity (venous. vs. washed blood at 6 h P > 0.05).
  
  
  CONCLUSIONS
  Compared with venous RBC, washed RBC from intraoperative cell salvage exhibited good oxygen-carrying and antioxidant capacities. However, the oxygen-carrying capacity of washed RBC that were allowed to stand for 6 h was not as good as that of venous RBC. Thus, we suggest that washed RBC that were left to stand for more than 6 h should not be reinfused.
authors.affiliation: Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, China.
authors.forename: J
authors.initials: J
authors.lastname: Che
authors.forename: M
authors.initials: M
authors.lastname: Tian
authors.forename: G
authors.initials: G
authors.lastname: Ding
authors.forename: Q
authors.initials: Q
authors.lastname: Huai
authors.forename: P
authors.initials: P
authors.lastname: Dong
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Li
mesh_terms: D019794:2,3-Diphosphoglycerate / Q000378:metabolism*; D000368:Aged; D001793:Blood Preservation; D002498:Centrifugation; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D057725:Operative Blood Salvage; D010100:Oxygen / Q000378:metabolism; D010718:Phosphatidylserines / Q000378:metabolism*; D013048:Specimen Handling
chemical_list: D010718:Phosphatidylserines; D019794:2,3-Diphosphoglycerate; D005954:Glucosephosphate Dehydrogenase; D010100:Oxygen