title: [The effect of biyuanshu oral liquid on the formation of Pseudomonas aeruginosa biofilms in vitro].
abstract: OBJECTIVE
  To observe the effect of biyuanshu oral liquid on the formation of pseudomonas aeruginosa biofilms in vitro.
  
  
  METHODS
  Pseudomonas aeruginosa biofilm was established by plate culture and detected by Scanning electron microscopy and AgNO3 staining. After treated with different dosages of biyuanshu oral liquid and erythromycin, the pseudomonas aeruginosa biofilms were observed by AgNO3 staining and the number of viable bacteria were measured by serial dilution.
  
  
  RESULTS
  The pseudomonas aeruginosa biofilms could be detected by SEM at the seventh culture day and it was consistent with the detection of AgNO3 staining. The biyuanshu oral liquid and erythromycin have the effect on inhibiting the formation of pseudomonas aeruginosa biofilms. But with the already formed pseudomonas aeruginosa biofilms the inhibition was not significant. The serial dilution method showed that the viable counts of bacteria of biyuanshu oral liquid and erythromycin treated groups were significantly lower than those untreated groups (P < 0.05).
  
  
  CONCLUSIONS
  The biyuanshu oral liquid and erythromycin can inhibit the formation of pseudomonas aeruginosa biofilms in vitro.
authors.affiliation: Department of Otolaryngology, the First Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, 310000, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Liu
authors.forename: Haihong
authors.initials: H
authors.lastname: Chen
authors.forename: Shengqing
authors.initials: S
authors.lastname: Wang
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004917:Erythromycin / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D004365:Drugs, Chinese Herbal; D004917:Erythromycin