title: Structural studies of human insulin cocrystallized with phenol or resorcinol via powder diffraction.
abstract: The effects of the ligands phenol and resorcinol on the crystallization of human insulin have been investigated as a function of pH. Powder diffraction data were used to characterize several distinct polymorphic forms. A previously unknown polymorph with monoclinic symmetry (P2(1)) was identified for both types of ligand with similar characteristics [the unit-cell parameters for the insulin-resorcinol complex were a = 114.0228 (8), b = 335.43 (3), c = 49.211 (6) Å, β = 101.531 (8)°].
authors.affiliation: Department of Biology, Section of Genetics, Cell Biology and Development, University of Patras, Patras, Greece.
authors.forename: Fotini
authors.initials: F
authors.lastname: Karavassili
authors.forename: Anastasia E
authors.initials: AE
authors.lastname: Giannopoulou
authors.forename: Eleni
authors.initials: E
authors.lastname: Kotsiliti
authors.forename: Lisa
authors.initials: L
authors.lastname: Knight
authors.forename: Mathias
authors.initials: M
authors.lastname: Norrman
authors.forename: Gerd
authors.initials: G
authors.lastname: Schluckebier
authors.forename: Lene
authors.initials: L
authors.lastname: Drube
authors.forename: Andrew N
authors.initials: AN
authors.lastname: Fitch
authors.forename: Jonathan P
authors.initials: JP
authors.lastname: Wright
authors.forename: Irene
authors.initials: I
authors.lastname: Margiolaki
mesh_terms: D003460:Crystallization; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000737:chemistry*; D019800:Phenol / Q000737:chemistry*; D033362:Powder Diffraction; D011487:Protein Conformation; D012118:Resorcinols / Q000737:chemistry*
chemical_list: D007328:Insulin; D012118:Resorcinols; D019800:Phenol; C031389:resorcinol