title: New method for isolating antibiotic-producing fungi.
abstract: A convenient and efficient method was established for isolating antifungal antibiotic-producing fungi from soil samples. In this method, soil samples were diluted and directly plated in agar medium by the standard fungi-isolating method, and the plates were cultured at 27 °C for 2-3 days to permit the growth of fungal colonies. Then, the suspension of pathogenic Candida albicans in saline (40 μl, 5-10 × 10⁵ CFU ml⁻¹) was overlaid by spraying on the plates under controlled conditions in the safety cabinet. After 1-day incubation, fungal colonies showing an antagonistic effect with the inhibition zone against sprayed C. albicans were selected. Among 151 isolates, 26 strains were found to reproduce anti-C. albicans activity in liquid medium, yielding a higher selection rate (17.2%) than that (3.1%) by the traditional method. This new method can be applied for isolation of microorganisms (fungi and actinomycetes) that produce antibiotics active against pathogenic microorganisms.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Mio
authors.initials: M
authors.lastname: Kawaguchi
authors.forename: Kenichi
authors.initials: K
authors.lastname: Nonaka
authors.forename: Rokuro
authors.initials: R
authors.lastname: Masuma
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tomoda
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D002176:Candida albicans / Q000302:isolation & purification* / Q000378:metabolism; D015169:Colony Count, Microbial; D003470:Culture Media; D005285:Fermentation; D012988:Soil Microbiology
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media