title: [Effects of Baicalin on an orthotopic transplantation mouse model of mismatch repair gene deficient colorectal cancer].
abstract: OBJECTIVE
  To study the anticancer effects of Baicalin on an orthotopic transplantation mouse model of mismatch repair gene deficient colorectal cancer.
  
  
  METHODS
  Sixty orthotopic transplantation mice model of human colon cancer cell line HCT-116 expressing eGFP were established, which were divided randomly into negative controlled group (5% NaHCO3) and 50, 100, 200 mg/kg Baicalin groups. The nude mice were treated with intragastric infusion twice a day. Nude mice growth state, average weigh, inhibition rate of transplanted tumor, tumor metastasis and survival state were observed.
  
  
  RESULTS
  At 14, 21 and 28 days after treatment with different dose of Baicalin, tumor growth velocity was significantly slower in the treatment groups, and tumor volume was significantly smaller than the controlled group (there were (832 ± 637), (2012 ± 1566) and (2494 ± 1557) mm(3) respectively in 14, 21 and 28 days) (F = 4.433, P < 0.05). At the end point of study, survival state of 100 mg/kg group (13/15) was superior to controlled group (8/15) and 200 mg/kg group (8/15) (χ(2) = 4.665 and 3.980, P < 0.05).However, there were no significant differences in tumor metastasis and tumor surface vessel density.
  
  
  CONCLUSIONS
  Baicalin has statistically significant effects in inhibiting tumor growth in an orthotopic transplantation mouse model of mismatch repair gene deficient colorectal cancer, and 100 mg/kg may be an ideal treatment dose.
authors.affiliation: Department of Colorectal Surgery, Nanjing University of Chinese Medicine, Nanjing, China. blyang1971@163.com
authors.forename: Bai-lin
authors.initials: BL
authors.lastname: Yang
authors.forename: Hong-jin
authors.initials: HJ
authors.lastname: Chen
authors.forename: Yu-gen
authors.initials: YG
authors.lastname: Chen
authors.forename: Yun-fei
authors.initials: YF
authors.lastname: Gu
authors.forename: Shu-peng
authors.initials: SP
authors.lastname: Zhang
authors.forename: Qiu
authors.initials: Q
authors.lastname: Lin
authors.forename: Ping
authors.initials: P
authors.lastname: Zhu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D000818:Animals; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D004195:Disease Models, Animal; D005419:Flavonoids / Q000008:administration & dosage / Q000627:therapeutic use*; D017353:Gene Deletion; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D000070957:MutL Protein Homolog 1; D009687:Nuclear Proteins / Q000235:genetics*; D023041:Xenograft Model Antitumor Assays
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D005419:Flavonoids; C501486:MLH1 protein, human; D009687:Nuclear Proteins; C038044:baicalin; D000070957:MutL Protein Homolog 1