title: Biochemical and functional characterization of a C-type lectin (BpLec) from Bothrops pauloensis snake venom.
abstract: In the present work, we report the isolation and partial biochemical characterization of BpLec, a C-type lectin purified from Bothrops pauloensis venom by one chromatographic step on an affinity agarose column immobilized with d-galactose. This protein was homogeneous by SDS-PAGE under reducing and nonreducing conditions, and was shown to be a 33.6 kDa homodimer by MALDI TOF analysis. BpLec presented an isoeletric point of 5.36. Its partial sequence of 132 amino acids for each subunit, determined by Edman degradation, revealed high identity (between 86% and 95%) when aligned with sequences of other related proteins. BpLec was capable of agglutinating native dog and cat erythrocytes and this activity was inhibited by β-galactosides and EDTA. Its hemagglutinating activity was abolished at high temperatures and stable in any pH range. BpLec was effective in inhibiting Gram-positive but not Gram-negative bacteria. In addition, BpLec agglutinated promastigote forms of Leishmania (Leishmania) amazonensis.
authors.affiliation: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia-MG, Brazil.
authors.forename: Letícia Eulálio
authors.initials: LE
authors.lastname: Castanheira
authors.forename: Débora Cristina de Oliveira
authors.initials: DC
authors.lastname: Nunes
authors.forename: Thomaz Monteiro
authors.initials: TM
authors.lastname: Cardoso
authors.forename: Paula de Souza
authors.initials: Pde S
authors.lastname: Santos
authors.forename: Luiz Ricardo
authors.initials: LR
authors.lastname: Goulart
authors.forename: Renata Santos
authors.initials: RS
authors.lastname: Rodrigues
authors.forename: Michael
authors.initials: M
authors.lastname: Richardson
authors.forename: Márcia Helena
authors.initials: MH
authors.lastname: Borges
authors.forename: Kelly Aparecida Geraldo
authors.initials: KA
authors.lastname: Yoneyama
authors.forename: Veridiana M
authors.initials: VM
authors.lastname: Rodrigues
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001419:Bacteria / Q000187:drug effects; D017837:Bothrops / Q000378:metabolism*; D002241:Carbohydrates / Q000494:pharmacology; D002415:Cats; D004285:Dogs; D004492:Edetic Acid / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects; D006384:Hemagglutination / Q000187:drug effects; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D037181:Lectins, C-Type / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D007891:Leishmania / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D012910:Snake Venoms / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D002241:Carbohydrates; D037181:Lectins, C-Type; D012910:Snake Venoms; D004492:Edetic Acid