title: Characterization of a gene coding for a putative adenosine deaminase-related growth factor by RNA interference in the basidiomycete Flammulina velutipes.
abstract: A full-length cDNA coding for a putative adenosine deaminase (Fv-ada) was isolated from the basidiomycete Flammulina velutipes. Fv-ada encodes a polypeptide consisting of 537 amino acid residues, which has a consensus sequence conserved among adenosine deaminase-related growth factors (ADGF) found in several metazoa, including chordates and insects. Fv-ada transcript was detected at all stages of growth in dikaryotic F. velutipes cells, with a peak at the primordial stage. Heterologous expression of Fv-ada in the yeast Pichia pastoris produced recombinant Fv-ADA that catalyzed the conversion of adenosine to inosine. Dikaryotic mycelia from F. velutipes were transformed with the binary plasmid pFungiway-Fv-ada, which was designed to suppress the expression of Fv-ada through RNA interference. The growth rates of the resulting transformants were retarded in response to the degree of suppression, indicating that Fv-ada plays an important role in the mycelial growth of F. velutipes. These results suggested that ADGF could function as growth factors in fungi, as is seen in other eukaryotes.
authors.affiliation: Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Sekiya
authors.forename: Masato
authors.initials: M
authors.lastname: Yamada
authors.forename: Kou
authors.initials: K
authors.lastname: Shibata
authors.forename: Toru
authors.initials: T
authors.lastname: Okuhara
authors.forename: Masumi
authors.initials: M
authors.lastname: Yoshida
authors.forename: Satoshi
authors.initials: S
authors.lastname: Inatomi
authors.forename: Goro
authors.initials: G
authors.lastname: Taguchi
authors.forename: Makoto
authors.initials: M
authors.lastname: Shimosaka
mesh_terms: D000243:Adenosine Deaminase / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D000595:Amino Acid Sequence; D055393:Flammulina / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D008969:Molecular Sequence Data; D025282:Mycelium / Q000254:growth & development; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D017386:Sequence Homology, Amino Acid
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D000243:Adenosine Deaminase