title: A preliminary search for alternatives to albumin as a medium supplement for the culture of human sperm.
abstract: Non-animal macromolecules (Select Phytone™ UF, wheat peptone, dextran 40, hydroxyethyl starch and methyl cellulose) as an alternative medium supplement for human spermatozoa were compared to bovine serum albumin. Select Phytone™ UF and wheat peptone discolored the medium and smelled like broth, making them unlikely to be acceptable for clinical use, whilst the others were colorless and odorless. All supplements were effective in the yield of spermatozoa isolated by swim-up technique, and maintenance of sperm motility. In summary, there are non-animal macromolecules that will support short-term sperm culture.
authors.affiliation: Fertility North, Joondalup, Western Australia, Australia.
authors.forename: Phillip
authors.initials: P
authors.lastname: Matson
authors.forename: Steve
authors.initials: S
authors.lastname: Tardif
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D008297:Male; D012662:Semen Preservation / Q000379:methods; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D013075:Sperm Capacitation; D013081:Sperm Motility; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology*
chemical_list: D003470:Culture Media; D012710:Serum Albumin, Bovine