title: The comparison of multilineage differentiation of bone marrow and adipose-derived mesenchymal stem cells.
abstract: BACKGROUND
  To compare the roles of adipose and bone marrow derived mesenchymal stem cells (AMSCs and BMSCs) in multiple differentiation capacity to provide a theoretical basis for stem cell transplantation.
  
  
  METHODS
  We isolated bone marrow and adipose derived mesenchymal stem cells and compared their phenotype, cell doubling time, the secretion of factors, and the ability of multi-differentiation.
  
  
  RESULTS
  BMSCs and AMSCs were similar in cell phenotype and the differences existed only between the expression of CD106. The proliferation rate of AMSCs was faster than of BMSCs (doubling time 28h vs. 39h) and the capacity to suppress T cell proliferation and activation was weakened in AMSCs. In addition, both sources of cells were able to differentiate into bone, fat, and cartilage which proved their stem cell properties.
  
  
  CONCLUSIONS
  Cell origin and abundance were decisive factors in stem cell applications and with the same premise as for AMSCs and BMSCs, adipose tissue is a more promising source of stem cells.
authors.affiliation: Clinical Research Center, Affiliated Hospital of Guangdong Medical College, China.
authors.forename: Xishan
authors.initials: X
authors.lastname: Zhu
authors.forename: Jing
authors.initials: J
authors.lastname: Du
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
mesh_terms: D050156:Adipogenesis / Q000502:physiology; D000273:Adipose Tissue / Q000166:cytology* / Q000502:physiology; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology* / Q000502:physiology; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D038081:Organogenesis / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology; D010641:Phenotype; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism; D055815:Young Adult
chemical_list: D015415:Biomarkers; D019010:Vascular Cell Adhesion Molecule-1