title: 2D difference gel electrophoresis reference map of a Fusarium graminearum nivalenol producing strain.
abstract: Fusarium graminearum is widely studied as a model for toxin production among plant pathogenic fungi. A 2D DIGE reference map for the nivalenol-producing strain 453 was established. Based on a whole protein extract, all reproducible spots were systematically picked and analyzed by MALDI-TOF/TOF, leading to the identification of 1102 protein species. The obtained map contributes to the annotation of the genome by identifying previously nondescribed hypothetical proteins and will serve as a reference for future studies aiming at deciphering F. graminearum biology and chemotype diversity.
authors.affiliation: Centre de Recherche Public-Gabriel Lippmann, Belvaux, Luxembourg.
authors.forename: Matias
authors.initials: M
authors.lastname: Pasquali
authors.forename: Tommaso
authors.initials: T
authors.lastname: Serchi
authors.forename: Jenny
authors.initials: J
authors.lastname: Renaut
authors.forename: Lucien
authors.initials: L
authors.lastname: Hoffmann
authors.forename: Torsten
authors.initials: T
authors.lastname: Bohn
mesh_terms: D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005656:Fungal Proteins / Q000032:analysis* / Q000737:chemistry*; D005670:Fusarium / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000032:analysis* / Q000737:chemistry*; D015203:Reproducibility of Results; D014255:Trichothecenes / Q000096:biosynthesis*
chemical_list: D005656:Fungal Proteins; D020543:Proteome; D014255:Trichothecenes; C038405:nivalenol