title: Increased procoagulant function of microparticles in pediatric inflammatory bowel disease: role in increased thrombin generation.
abstract: OBJECTIVE
  Patients with inflammatory bowel disease (IBD) have a higher risk for venous thromboembolism compared with non-IBD subjects. The pathogenic mechanisms of the thrombotic events are not fully understood. We investigated levels of circulating microparticles and their influence on thrombin generation in pediatric patients with IBD during active and quiescent disease compared with healthy controls.
  
  
  METHODS
  Plasma samples were collected from 33 pediatric patients with Crohn disease (CD), 20 pediatric patients with ulcerative colitis (UC), and 60 healthy controls. Microparticles' procoagulant activity was measured by enzyme-linked immunosorbent assay, and the dependency of thrombin generation on microparticles-derived tissue factor was determined by means of calibrated automated thrombography.
  
  
  RESULTS
  The procoagulant function of microparticles was significantly increased in patients with active and inactive CD, and active UC compared with controls. Endogenous thrombin potential was significantly higher in patients with CD and UC compared with controls. A minor influence of microparticles on thrombin generation was only observed for patients with active UC.
  
  
  CONCLUSIONS
  Our study shows increased procoagulant function of microparticles in pediatric patients with active and quiescent CD and active UC compared with controls, but demonstrates that they are not a major cause for the higher thrombin generation in pediatric patients with IBD.
authors.affiliation: Department of Pediatrics and Adolescent Medicine, Medical University of Graz, Auenbruggerplatz 34/2, Graz, Austria.
authors.forename: Andrea
authors.initials: A
authors.lastname: Deutschmann
authors.forename: Axel
authors.initials: A
authors.lastname: Schlagenhauf
authors.forename: Bettina
authors.initials: B
authors.lastname: Leschnik
authors.forename: Karl Martin
authors.initials: KM
authors.lastname: Hoffmann
authors.forename: Almuthe
authors.initials: A
authors.lastname: Hauer
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Muntean
mesh_terms: D000293:Adolescent; D001317:Austria / Q000453:epidemiology; D001777:Blood Coagulation; D001780:Blood Coagulation Tests; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D002648:Child; D015331:Cohort Studies; D003093:Colitis, Ulcerative / Q000097:blood / Q000378:metabolism / Q000503:physiopathology*; D003424:Crohn Disease / Q000097:blood / Q000378:metabolism / Q000503:physiopathology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D011516:Prothrombin / Q000378:metabolism; D012306:Risk; D012720:Severity of Illness Index; D013917:Thrombin / Q000378:metabolism*; D013925:Thromboplastin / Q000378:metabolism*; D054556:Venous Thromboembolism / Q000453:epidemiology / Q000209:etiology*
chemical_list: D011516:Prothrombin; D013925:Thromboplastin; D013917:Thrombin