title: [Comparison of Kinyoun, auramine O, and Ziehl-Neelsen staining for diagnosing tuberculosis at the National Tuberculosis Center in Burkina Faso].
abstract: BACKGROUND
  This study was conducted at the National Tuberculosis Center in Burkina Faso from October 2007 through May 2008.
  
  
  OBJECTIVE
  Our objective was to compare the diagnostic performance of three staining methods: Kinyoun, auramine O, and Ziehl-Neelsen.
  
  
  METHODS
  Ziehl-Neelsen staining served as the reference method to assess the diagnostic performance of Kinyoun and auramine O staining. In all, 616 sputum smears from 233 patients were read with each method to detect acid-fast bacilli. SPSS was used for data analysis.
  
  
  RESULTS
  The results of auramine O staining showed positive diagnoses in 15.9% of the samples; sensitivity was 100%, specificity 95.6%, and the positive and negative predictive values 75.7% and 100% respectively. Kinyoun staining produced a positive diagnosis rate of 12%, sensitivity of 96.4%, specificity of 99.5%, and positive and negative predictive values of 96.4% and 99.5%.
  
  
  CONCLUSIONS
  Our study indicates that auramine O staining had a better sensitivity for detecting acid-fast bacilli than Kinyoun staining. Accordingly, the use of auramine O staining should increase the detection rate for pulmonary tuberculosis in Burkina Faso.
authors.affiliation: Centre national de lutte antituberculeuse. sawtileon@yahoo.fr
authors.forename: T L
authors.initials: TL
authors.lastname: Sawadogo
authors.forename: L G B
authors.initials: LG
authors.lastname: Savadogo
authors.forename: S
authors.initials: S
authors.lastname: Diande
authors.forename: F
authors.initials: F
authors.lastname: Ouedraogo
authors.forename: A
authors.initials: A
authors.lastname: Mourfou
authors.forename: A
authors.initials: A
authors.lastname: Gueye
authors.forename: I
authors.initials: I
authors.lastname: Sawadogo
authors.forename: B
authors.initials: B
authors.lastname: Nebié
authors.forename: L
authors.initials: L
authors.lastname: Sangare
authors.forename: A S
authors.initials: AS
authors.lastname: Ouattara
mesh_terms: D001576:Benzophenoneidum; D002050:Burkina Faso; D004396:Coloring Agents; D006801:Humans; D013183:Sputum / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
chemical_list: D004396:Coloring Agents; D001576:Benzophenoneidum