title: [Establishment of an isolated porcine liver machine perfusion model for high-intensity focused ultrasound studies].
abstract: OBJECTIVE
  To explore the feasibility of establishing an isolated porcine liver machine perfusion model and assess its value in high-intensity focused ultrasound studies.
  
  
  METHODS
  Twenty-one isolated porcine livers were perfused with autologous blood for 4 h through dual vessels (portal vein and hepatic artery) cannulation using an extracorporeal circulation machine under a sub-normothermic perfusion condition. The perfusion model was assessed by monitoring the liver color, texture, liver weight gain, hemodynamic parameters, color Doppler flow imaging, bile output and histopathology.
  
  
  RESULTS
  Nineteen isolated porcine livers were successfully cannulated with dual vessels, and failure of hepatic artery intubation occurred in two porcine livers. After machine perfusion for 4 h, the isolated livers maintained a soft texture with stable hemodynamic levels within relative normal physiological ranges. The bile output was more than 3 ml/h within the initial 3 h of perfusion. Histopathological examination demonstrated no morphological or structural changes of the liver tissues.
  
  
  CONCLUSIONS
  The isolated porcine liver perfusion model is stable and feasible, and can be used for high-intensity focused ultrasound studies.
authors.affiliation: Institute of Biomedical Engineering, Chongqing Medical University, Chongqing, China. burui0703@163.com
authors.forename: Rui
authors.initials: R
authors.lastname: Bu
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Zou
authors.forename: Li
authors.initials: L
authors.lastname: Yin
authors.forename: Feng
authors.initials: F
authors.lastname: Wu
authors.forename: Shihe
authors.initials: S
authors.lastname: Meng
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Jiao
authors.initials: J
authors.lastname: Jiao
authors.forename: Han
authors.initials: H
authors.lastname: Yang
mesh_terms: D000818:Animals; D004867:Equipment Design; D005112:Extracorporeal Circulation / Q000295:instrumentation* / Q000379:methods; D006439:Hemodynamics; D008099:Liver / Q000098:blood supply / Q000000981:diagnostic imaging*; D008102:Liver Circulation / Q000502:physiology; D013552:Swine; D014463:Ultrasonography