title: Subpopulation-proteomics in prokaryotic populations.
abstract: Clonal microbial cells do not behave in an identical manner and form subpopulations during cultivation. Besides varying micro-environmental conditions, cell inherent features like cell cycle dependent localization and concentration of regulatory proteins as well as epigenetic properties are well accepted mechanisms creating cell heterogeneity. Another suspected reason is molecular noise on the transcriptional and translational level. A promising tool to unravel reasons for cell heterogeneity is the combination of cell sorting and subpopulation proteomics. This review summarizes recent developments in prokaryotic single-cell analytics and provides a workflow for selection of single cells, low cell number mass spectrometry, and proteomics evaluation. This approach is useful for understanding the dependency of individual cell decisions on inherent protein profiles.
authors.affiliation: Helmholtz Centre for Environmental Research - UFZ, Department for Environmental Microbiology, Permoserstr. 15, 04318 Leipzig, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Jahn
authors.forename: Jana
authors.initials: J
authors.lastname: Seifert
authors.forename: Martin
authors.initials: M
authors.lastname: von Bergen
authors.forename: Andreas
authors.initials: A
authors.lastname: Schmid
authors.forename: Bruno
authors.initials: B
authors.lastname: Bühler
authors.forename: Susann
authors.initials: S
authors.lastname: Müller
mesh_terms: D002999:Clone Cells / Q000166:cytology / Q000378:metabolism; D013058:Mass Spectrometry; D011387:Prokaryotic Cells / Q000166:cytology* / Q000378:metabolism*; D020543:Proteome / Q000032:analysis / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D059010:Single-Cell Analysis / Q000379:methods*
chemical_list: D020543:Proteome