title: Serotypes and virulence profiles of atypical enteropathogenic Escherichia coli (EPEC) isolated from bovine farms and abattoirs.
abstract: OBJECTIVE
  The objective of this study was to examine the prevalence of enteropathogenic Escherichia coli (EPEC) on beef and dairy farms and in beef abattoirs and to characterize the isolates in terms of serogroup and virulence markers.
  
  
  RESULTS
  Bovine faecal samples (n = 1200), farm soil samples (n = 600), hide samples (n = 450) and carcass samples (n = 450) were collected from 20 farms and three abattoirs throughout Ireland over a 12-month period. After selective enrichment, samples testing positive for the intimin gene (eae) using PCR screening were cultured, and colonies were examined for the presence of the eae, vt(1) and vt(2) genes. Colonies that were positive for the intimin gene and negative for the verotoxin genes were further screened using PCR for a range of virulence factors including tir, espA, espB katP, espP, etpD, saa, sab, toxB, iha, lpfA(O157/OI-141) , lpfA(O113) and lpfA(O157/OI-154) . PCR screening was also used to screen for variations in the intimin gene (eae). Of the 2700 source samples analysed, 3.9% (47 of 1200) of faecal, 2% (12 of 600) of soil, 6.4% (29 of 450) of hide and 0.7% (3 of 450) of carcass samples were PCR positive (for the presence of the eae gene). All 140 isolates obtained were atypical EPEC (aEPEC), while θ and β intimin types were common. The virulence factors hlyA, tir, lpfA (O113) , lpfA (O157/OI-154) , and iha were frequently detected, while lpfA(O157/OI-141) , saa, espA, espB and toxB were also present but to a lesser extent.
  
  
  CONCLUSIONS
  It was concluded that cattle are a source of aEPEC, many of which have the virulence machinery necessary to be pathogenic to humans.
  
  
  CONCLUSIONS
  These findings suggest the need for increased research on aEPEC with particular emphasis on food safety and public health risk.
authors.affiliation: Department of Food Safety, Ashtown Food Research Centre, Teagasc, Ashtown, Dublin, Ireland.
authors.forename: Á
authors.initials: Á
authors.lastname: Monaghan
authors.forename: B
authors.initials: B
authors.lastname: Byrne
authors.forename: S
authors.initials: S
authors.lastname: Fanning
authors.forename: T
authors.initials: T
authors.lastname: Sweeney
authors.forename: D
authors.initials: D
authors.lastname: McDowell
authors.forename: D J
authors.initials: DJ
authors.lastname: Bolton
mesh_terms: D000003:Abattoirs; D000818:Animals; D002417:Cattle / Q000382:microbiology*; D054308:Enteropathogenic Escherichia coli / Q000145:classification* / Q000302:isolation & purification / Q000472:pathogenicity*; D005243:Feces / Q000382:microbiology; D007494:Ireland; D008460:Meat; D016133:Polymerase Chain Reaction; D012703:Serotyping; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics
chemical_list: D037521:Virulence Factors