title: WHAMM is required for meiotic spindle migration and asymmetric cytokinesis in mouse oocytes.
abstract: WASP homolog associated with actin, membranes and microtubules (WHAMM) is a newly discovered nucleation-promoting factor that links actin and microtubule cytoskeleton and regulates transport from the endoplasmic reticulum to the Golgi apparatus. However, knowledge of WHAMM is limited to interphase somatic cells. In this study, we examined its localization and function in mouse oocytes during meiosis. Immunostaining showed that in the germinal vesicle (GV) stage, there was no WHAMM signal; after meiosis resumption, WHAMM was associated with the spindle at prometaphase I (Pro MI), metaphase I (MI), telophase I (TI) and metaphase II (MII) stages. Nocodazole and taxol treatments showed that WHAMM was localized around the MI spindle. Depletion of WHAMM by microinjection of specific short interfering (si)RNA into the oocyte cytoplasm resulted in failure of spindle migration, disruption of asymmetric cytokinesis and a decrease in the first polar body extrusion rate during meiotic maturation. Moreover, actin cap formation was also disrupted after WHAMM depletion, confirming the failure of spindle migration. Taken together, our data suggest that WHAMM is required for peripheral spindle migration and asymmetric cytokinesis during mouse oocyte maturation.
authors.affiliation: Organ Transplantation Institute, Xiamen University, Xiamen, Fujian, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.forename: Lu
authors.initials: L
authors.lastname: Ding
authors.forename: Rui
authors.initials: R
authors.lastname: Pan
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Ma
authors.forename: Pan-Pan
authors.initials: PP
authors.lastname: Cheng
authors.forename: Chun-Hui
authors.initials: CH
authors.lastname: Zhang
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Shen
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: He
authors.forename: Zhong-Quan
authors.initials: ZQ
authors.lastname: Qi
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Wang
mesh_terms: D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins; D002478:Cells, Cultured; D048749:Cytokinesis; D003598:Cytoskeletal Proteins; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005260:Female; D006056:Golgi Apparatus / Q000378:metabolism; D008540:Meiosis; D051379:Mice; D008813:Mice, Inbred ICR; D008870:Microtubules / Q000378:metabolism*; D015739:Nocodazole / Q000494:pharmacology; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism*; D017239:Paclitaxel / Q000494:pharmacology; D034622:RNA Interference; D034741:RNA, Small Interfering; D008941:Spindle Apparatus / Q000378:metabolism*; D050257:Tubulin Modulators / Q000494:pharmacology
chemical_list: D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D034741:RNA, Small Interfering; D050257:Tubulin Modulators; C491671:Waspip protein, mouse; D017239:Paclitaxel; D015739:Nocodazole