title: Synapse-binding subpopulations of Aβ oligomers sensitive to peptide assembly blockers and scFv antibodies.
abstract: Amyloid β42 self-assembly is complex, with multiple pathways leading to large insoluble fibrils or soluble oligomers. Oligomers are now regarded as most germane to Alzheimer's pathogenesis. We have investigated the hypothesis that oligomer formation itself occurs through alternative pathways, with some leading to synapse-binding toxins. Immediately after adding synthetic peptide to buffer, solutions of Aβ42 were separated by a 50 kDa filter and fractions assessed by SDS-PAGE silver stain, Western blot, immunoprecipitation, and capacity for synaptic binding. Aβ42 rapidly assembled into aqueous-stable oligomers, with similar protein abundance in small (<50 kDa) and large (>50 kDa) oligomer fractions. Initially, both fractions were SDS-labile and resolved into tetramers, trimers, and monomers by SDS-PAGE. Upon continued incubation, the larger oligomers developed a small population of SDS-stable 10-16mers, and the smaller oligomers generated gel-impermeant complexes. The two fractions associated differently with neurons, with prominent synaptic binding limited to larger oligomers. Even within the family of larger oligomers, synaptic binding was associated with only a subset of these species, as a new scFv antibody (NUsc1) immunoprecipitated only a small portion of the oligomers while eliminating synaptic binding. Interestingly, low doses of the peptide KLVFFA blocked assembly of the 10-16mers, and this result was associated with loss of the smaller clusters of oligomers observed at synaptic sites. What distinguishes these smaller clusters from the unaffected larger clusters is not yet known. Results indicate that distinct species of Aβ oligomers are generated by alternative assembly pathways and that synapse-binding subpopulations of Aβ oligomers could be specifically targeted for Alzheimer's therapeutics.
authors.affiliation: Department of Neurobiology, Department of Chemistry, Recombinant Protein Production Core, and Departments of Molecular Biosciences and Radiology, Northwestern University, Evanston, Illinois 60208, United States.
authors.forename: Pauline T
authors.initials: PT
authors.lastname: Velasco
authors.forename: Marie C
authors.initials: MC
authors.lastname: Heffern
authors.forename: Adriano
authors.initials: A
authors.lastname: Sebollela
authors.forename: Izolda A
authors.initials: IA
authors.lastname: Popova
authors.forename: Pascale N
authors.initials: PN
authors.lastname: Lacor
authors.forename: Kevin B
authors.initials: KB
authors.lastname: Lee
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Sun
authors.forename: Benjamin N
authors.initials: BN
authors.lastname: Tiano
authors.forename: Kirsten L
authors.initials: KL
authors.lastname: Viola
authors.forename: Amanda L
authors.initials: AL
authors.lastname: Eckermann
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Meade
authors.forename: William L
authors.initials: WL
authors.lastname: Klein
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D047468:Immunoprecipitation; D010446:Peptide Fragments / Q000494:pharmacology*; D057127:Single-Chain Antibodies / Q000494:pharmacology*; D013569:Synapses / Q000737:chemistry*
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D057127:Single-Chain Antibodies