title: Single laboratory evaluation of a planar waveguide-based system for a simple simultaneous analysis of four mycotoxins in wheat.
abstract: The accuracy and precision of a commercially available system based on an indirect competitive immunoassay and planar waveguide technology was evaluated for the analysis of deoxynivalenol (DON), ochratoxin A (OTA), zearalenone (ZEAR), and T-2 toxin in wheat. The system generally performed well at the tested concentrations that were close to the regulatory limits of DON and OTA in wheat. The mean percent recovery of OTA from certified and in-house reference materials ranged from 90 to 111 %, with a relative standard deviation of 8-16 % (at 4.2, 4.9, and 7.0 μg/kg). Mean percent recoveries of DON ranged from 75 to 103 %, with a relative standard deviation of 14-20 % (at 610, 940, and 1300 μg/kg). As analyte concentrations approached the lower limits of the working range of 3 μg/kg OTA and 400 μg/kg DON, the mean percent recoveries and relative standard deviation increased for both DON and OTA. A lack of reference materials precluded a thorough evaluation of the method for the analysis of ZEAR and T-2. The particular strength of the technology was that multiple mycotoxins were analyzed simultaneously.
authors.affiliation: Grain Research Laboratory, Canadian Grain Commission, Winnipeg, MB R3C 3G8, Canada. sheryl.tittlemier@grainscanada.gc.ca
authors.forename: Sheryl A
authors.initials: SA
authors.lastname: Tittlemier
authors.forename: Mike
authors.initials: M
authors.lastname: Roscoe
authors.forename: Dainna
authors.initials: D
authors.lastname: Drul
authors.forename: Richard
authors.initials: R
authors.lastname: Blagden
authors.forename: Colleen
authors.initials: C
authors.lastname: Kobialka
authors.forename: Jason
authors.initials: J
authors.lastname: Chan
authors.forename: Don
authors.initials: D
authors.lastname: Gaba
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D007118:Immunoassay / Q000379:methods; D009183:Mycotoxins / Q000032:analysis*; D014908:Triticum / Q000737:chemistry*
chemical_list: D009183:Mycotoxins