title: Kaempferol glycosides from the twigs of Cinnamomum osmophloeum and their nitric oxide production inhibitory activities.
abstract: In the present study, ethanolic extract of twigs from Cinnamomum osmophloeum led to isolate nine kaempferol glycosides including two new kaempferol triglycosides that were characterized as kaempferol 3-O-β-D-xylopyranosyl-(1→2)-α-L-arabinofuranosyl-7-O-α-L-rhamnopyranoside (1) and kaempferol 3-O-β-D-xylopyranosyl-(1→2)-α-L-rhamnopyranosyl-7-O-α-L-rhamnopyranoside (2). The structures of these compounds were assigned by the application of 1D and 2D NMR spectroscopy and other techniques. Among these nine compounds, kaempferol 7-O-α-L-rhamnopyranoside (9) revealed inhibitory effect against LPS-induced production of nitric oxide in RAW 264.7 macrophages with an IC(50) value of 41.2 μM. It also slightly reduced PGE(2) accumulation by 26% at the concentration of 50 μM.
authors.affiliation: School of Forestry and Resource Conservation, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Huan-You
authors.initials: HY
authors.lastname: Lin
authors.forename: Shang-Tzen
authors.initials: ST
authors.lastname: Chang
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D027424:Cinnamomum / Q000737:chemistry*; D015232:Dinoprostone / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D044949:Kaempferols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000737:chemistry / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D006027:Glycosides; D044949:Kaempferols; D008070:Lipopolysaccharides; C516876:kaempferol-7-O-alpha-L-rhamnopyranoside; D009569:Nitric Oxide; D000431:Ethanol; D015232:Dinoprostone