title: Lysine-directed staining of proteins for MS-based analyses.
abstract: Visualization of proteins and MS-based analyses are elemental tasks in modern biochemistry. Nevertheless, reports about covalent protein dyes and their suitability for subsequent MS experiments remain scarce. In a recent work, we demonstrated that covalent prestaining of proteins with Uniblue A drastically speeds up proteomic workflows. The present study introduces dabsyl chloride as another truly MS-compatible protein stain. Remarkably, although Uniblue A and dabsyl chloride employ different nucleophilic reaction mechanisms, both are highly specific for lysine residues. The predictable peptide modifications allow easy integration into state-of-the-art bioinformatic workflows. Further, lysine-directed derivatizations with hydrophobic reagents such as dabsyl chloride complement the cysteine-directed ALiPHAT strategy for increasing the sensitivity of peptide identifications.
authors.affiliation: College of Agricultural Sciences, Oregon State University, Corvallis, OR, USA.
authors.forename: Matthew T
authors.initials: MT
authors.lastname: Yasui
authors.forename: Marco A
authors.initials: MA
authors.lastname: Mata-Gómez
authors.forename: Robert
authors.initials: R
authors.lastname: Winkler
mesh_terms: D000880:Anthraquinones / Q000737:chemistry; D004396:Coloring Agents / Q000737:chemistry*; D008239:Lysine / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D013451:Sulfonic Acids / Q000737:chemistry; D013696:Temperature; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D000880:Anthraquinones; D004396:Coloring Agents; D011506:Proteins; D013451:Sulfonic Acids; C096133:uniblue A; C010572:4-N,N-dimethylaminoazobenzene-4'-sulfonyl chloride; D004124:p-Dimethylaminoazobenzene; D008239:Lysine