title: Substrate channels revealed in the trimeric Lactobacillus reuteri bacterial microcompartment shell protein PduB.
abstract: Lactobacillus reuteri metabolizes two similar three-carbon molecules, 1,2-propanediol and glycerol, within closed polyhedral subcellular bacterial organelles called bacterial microcompartments (metabolosomes). The outer shell of the propanediol-utilization (Pdu) metabolosome is composed of hundreds of mainly hexagonal protein complexes made from six types of protein subunits that share similar domain structures. The structure of the bacterial microcompartment protein PduB has a tandem structural repeat within the subunit and assembles into a trimer with pseudo-hexagonal symmetry. This trimeric structure forms sheets in the crystal lattice and is able to fit within a polymeric sheet of the major shell component PduA to assemble a facet of the polyhedron. There are three pores within the trimer and these are formed between the tandem repeats within the subunits. The structure shows that each of these pores contains three glycerol molecules that interact with conserved residues, strongly suggesting that these subunit pores channel glycerol substrate into the metabolosome. In addition to the observation of glycerol occupying the subunit channels, the presence of glycerol on the molecular threefold symmetry axis suggests a role in locking closed the central region.
authors.affiliation: School of Biological and Chemical Sciences, Queen Mary University of London, 327 Mile End Road, London, England.
authors.forename: Allan
authors.initials: A
authors.lastname: Pang
authors.forename: Mingzhi
authors.initials: M
authors.lastname: Liang
authors.forename: Michael B
authors.initials: MB
authors.lastname: Prentice
authors.forename: Richard W
authors.initials: RW
authors.lastname: Pickersgill
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D001704:Biopolymers / Q000737:chemistry*; D003460:Crystallization; D005990:Glycerol / Q000378:metabolism; D052200:Limosilactobacillus reuteri / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D001704:Biopolymers; D005990:Glycerol