title: Difluoro-C4'-oxidized abasic site for efficient amine modification in biological systems.
abstract: An oligodeoxynucleotide (ODN) containing a 2',2'-difluorinated analogue of a C4'-oxidized abasic site (C4'-OAS) was designed for the amine modification of biomolecules that interact with nucleic acids. In contrast to the parent C4'-OAS, which yielded amine-modified products accompanied by DNA strand scission, the ODN containing the difluoro C4'-OAS efficiently yielded products carrying ODNs. The amine modification proceeded without additional reagents being required and might be applicable to reactions in biological systems.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.forename: Akiko
authors.initials: A
authors.lastname: Jinnouchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Suemune
authors.forename: Mariko
authors.initials: M
authors.lastname: Aso
mesh_terms: D000588:Amines / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D004249:DNA Damage; D006845:Hydrocarbons, Fluorinated / Q000138:chemical synthesis* / Q000737:chemistry / Q000528:radiation effects; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000528:radiation effects; D010084:Oxidation-Reduction
chemical_list: D000588:Amines; D006845:Hydrocarbons, Fluorinated; D009838:Oligodeoxyribonucleotides; D004247:DNA