title: Detection and differential diagnosis of colon cancer by a cumulative analysis of promoter methylation.
abstract: Alterations in the methylation of promoters of cancer-related genes are promising biomarkers for the early detection of disease. Compared with single methylation alteration, assessing combined methylation alterations can provide higher association with specific cancer. Here we use cationic conjugated polymer-based fluorescence resonance energy transfer to quantitatively analyse DNA methylation levels of seven colon cancer-related genes in a Chinese population. Through a stepwise discriminant analysis and cumulative detection of methylation alterations, we acquire high accuracy and sensitivity for colon cancer detection (86.3 and 86.7%) and for differential diagnosis (97.5 and 94%). Moreover, we identify a correlation between the CpG island methylator phenotype and clinically important parameters in patients with colon cancer. The cumulative analysis of promoter methylation alterations by the cationic conjugated polymer-based fluorescence resonance energy transfer may be useful for the screening and differential diagnosis of patients with colon cancer, and for performing clinical correlation analyses.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Yang
authors.forename: Ying
authors.initials: Y
authors.lastname: Dong
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Chunlei
authors.initials: C
authors.lastname: Zhu
authors.forename: Hui
authors.initials: H
authors.lastname: Chong
authors.forename: Jiangyang
authors.initials: J
authors.lastname: Lu
authors.forename: Dehai
authors.initials: D
authors.lastname: Yu
authors.forename: Libing
authors.initials: L
authors.lastname: Liu
authors.forename: Fengting
authors.initials: F
authors.lastname: Lv
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
mesh_terms: D000236:Adenoma / Q000175:diagnosis / Q000235:genetics; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics; D002412:Cations; D003110:Colonic Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D018899:CpG Islands / Q000235:genetics; D004247:DNA / Q000378:metabolism; D019175:DNA Methylation / Q000235:genetics*; D003937:Diagnosis, Differential; D005260:Female; D031541:Fluorescence Resonance Energy Transfer; D052138:Genes, Neoplasm / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D011108:Polymers / Q000737:chemistry; D011401:Promoter Regions, Genetic
chemical_list: D014408:Biomarkers, Tumor; D002412:Cations; D011108:Polymers; D004247:DNA