title: [Expression and role of urotensin II on the lung of patients with pulmonary hypertension with congenital heart disease].
abstract: OBJECTIVE
  To observe the expression of urotensin II (UII) on the lung of patients with pulmonary hypertension (PH) with congenital heart disease and investigate the meaning of this phenomenon.
  
  
  METHODS
  Thirty eight patients with CHD were divided into three groups according to pulmonary arterial systolic pressure (PASP) measured in cardiac catheterization and surgery: normal pulmonary pressure group (N group, PASP < 30 mm Hg, n = 10), mild PH group (M group, PASP ≥ 30 mm Hg, n = 15), severe or moderate PH group (S group, PASP ≥ 50 mm Hg, n = 13). The expression of UII protein and UII mRNA in pulmonary arterioles were measured separately by immunohistochemical (IHC) analysis and in situ hybridization (ISH) analysis.
  
  
  RESULTS
  (1) The results of UIIIHC staining: The UII protein expression of group M was higher than that of group N (20.22 ± 3.58 vs. 14.34 ± 2.18, P < 0.01), but less than group S (20.22 ± 3.58 vs. 28.92 ± 3.22, P < 0.05). (2) The results of UIIISH mRNA staining were similar to IHC staining, the A value of group M was higher than group N (12.51 ± 2.02 vs. 8.85 ± 1.41, P < 0.05), less than that of group S(12.51 ± 2.02 vs. 25.35 ± 4.33, P < 0.01). (3) Correlation study: there was a positive correlation between the A values of UIIIHC and pulmonary hypertension (r = 0.64, P < 0.01, n = 38), a positive correlation between the A values of UIIISH and pulmonary hypertension (r = 0.58, P < 0.01, n = 38).
  
  
  CONCLUSIONS
  There was the expression of Urotensin II protein and mRNA in the lung of pulmonary hypertension patients with congenital heart disease, and these expression may involve the formation of pulmonary hypertension of congenital heart disease.
authors.affiliation: Department of Pediatrics, Wenzhou Medical College, Wenzhou, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Rong
authors.forename: Hui-ping
authors.initials: HP
authors.lastname: Wu
authors.forename: Hui-xian
authors.initials: HX
authors.lastname: Qiu
authors.forename: Yue
authors.initials: Y
authors.lastname: Ren
authors.forename: Yuan-hai
authors.initials: YH
authors.lastname: Zhang
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.forename: Rong-zhou
authors.initials: RZ
authors.lastname: Wu
authors.forename: Xing-ti
authors.initials: XT
authors.lastname: Hu
mesh_terms: D000293:Adolescent; D001794:Blood Pressure; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D006330:Heart Defects, Congenital / Q000150:complications / Q000378:metabolism* / Q000503:physiopathology; D006801:Humans; D006976:Hypertension, Pulmonary / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007223:Infant; D008168:Lung / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D011651:Pulmonary Artery / Q000378:metabolism / Q000503:physiopathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012720:Severity of Illness Index; D014579:Urotensins / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D014579:Urotensins; C029677:urotensin II