title: The membrane-cytoskeletal protein 4.1N is involved in the process of cell adhesion, migration and invasion of breast cancer cells.
abstract: Protein 4.1N belongs to the protein 4.1 superfamily that links transmembrane proteins to the actin cytoskeleton. Recent evidence has shown that protein 4.1 is important in tumor suppression. However, the functions of 4.1N in the metastasis of breast cancer are largely unknown. In the present study, MCF-7, T-47D and MDA-MB-231 breast cancer cell lines with various metastatic abilities were employed. Protein 4.1N was found to be expressed in poorly metastatic MCF-7 and middle metastatic T-47D cell lines, and was predominantly associated with cell-cell junctions. However, no 4.1N expression was detected in the highly metastatic MDA-MB-231 cells. Moreover, re-expression of 4.1N in MDA-MB-231 cells inhibited cell adhesion, migration and invasion. The results suggest that protein 4.1N is a negative regulator of cell metastasis in breast cancer.
authors.affiliation: Department of Bioengineering, Zhengzhou University, Zhengzhou 450001; ; Henan Academy of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450052;
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Ji
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Shi
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Shi
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Zhou
authors.forename: Xilong
authors.initials: X
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Xiang
authors.initials: X
authors.lastname: Ji
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Gao
authors.forename: Yuanming
authors.initials: Y
authors.lastname: Qi
authors.forename: Qiaozhen
authors.initials: Q
authors.lastname: Kang