title: High endothelial venules (HEVs) in human melanoma lesions: Major gateways for tumor-infiltrating lymphocytes.
abstract: The presence of tumor-infiltrating lymphocytes (TILs) is a strong prognostic parameter for local dissemination and overall survival in melanoma. Lymphocyte migration from blood into peripheral tissues is mainly regulated by vascular endothelium. However, the blood vessels and mechanisms governing the recruitment of TILs in melanoma tumors remain poorly understood. Here, we show that high endothelial venules (HEVs), specialized blood vessels for lymphocyte extravasation into lymphoid tissues, are frequently found in melanoma tumors and are associated with high levels of lymphocyte infiltration. The analysis of 225 primary melanomas revealed that lymphocytes specifically infiltrated HEV-rich areas of melanoma tumors and that the density of MECA-79+ HEVs was variable among patients and strongly correlated with CD3+, CD8+ and CD20+ TIL densities. Inflammatory (CCL5, CXCL9, CXCL10 and CXCL11) and lymphoid (CCL21, CCL19 and CXCL13) chemokines as well as TH1 and naïve T-cell genes were overexpressed in melanoma samples with high densities of tumor HEVs. Mature dendritic cells (mDCs) were frequently found around tumor HEVs and densities of HEVs and DC-LAMP+ mDCs within tumor stroma were strongly correlated. DCs which maintain HEVs in lymph nodes, may thus also contribute to the regulation of HEVs in melanomas. Finally, we found significantly higher densities of tumor HEVs in melanomas with tumor regression, low Clark level of invasion and thin Breslow thickness (all p < 0.001). The strong association between tumor HEVs, TILs, mDCs and clinical parameters of melanoma, supports a critical role for HEVs in limiting malignant melanoma development through both naïve and effector T-lymphocyte recruitment and activation.
authors.affiliation: CNRS; IPBS (Institut de Pharmacologie et de Biologie Structurale); Toulouse, France ; Université de Toulouse; Toulouse, France ; Institut Claudius Regaud; Toulouse, France.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Martinet
authors.forename: Sophie
authors.initials: S
authors.lastname: Le Guellec
authors.forename: Thomas
authors.initials: T
authors.lastname: Filleron
authors.forename: Laurence
authors.initials: L
authors.lastname: Lamant
authors.forename: Nicolas
authors.initials: N
authors.lastname: Meyer
authors.forename: Philippe
authors.initials: P
authors.lastname: Rochaix
authors.forename: Ignacio
authors.initials: I
authors.lastname: Garrido
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Girard