title: A phenotypic screening platform to identify small molecule modulators of Chlamydomonas reinhardtii growth, motility and photosynthesis.
abstract: Chemical biology, the interfacial discipline of using small molecules as probes to investigate biology, is a powerful approach of developing specific, rapidly acting tools that can be applied across organisms. The single-celled alga Chlamydomonas reinhardtii is an excellent model system because of its photosynthetic ability, cilia-related motility and simple genetics. We report the results of an automated fitness screen of 5,445 small molecules and subsequent assays on motility/phototaxis and photosynthesis. Cheminformatic analysis revealed active core structures and was used to construct a naïve Bayes model that successfully predicts algal bioactive compounds.
authors.forename: Simon E
authors.initials: SE
authors.lastname: Alfred
authors.forename: Anuradha
authors.initials: A
authors.lastname: Surendra
authors.forename: Chris
authors.initials: C
authors.lastname: Le
authors.forename: Ken
authors.initials: K
authors.lastname: Lin
authors.forename: Alexander
authors.initials: A
authors.lastname: Mok
authors.forename: Iain M
authors.initials: IM
authors.lastname: Wallace
authors.forename: Michael
authors.initials: M
authors.lastname: Proctor
authors.forename: Malene L
authors.initials: ML
authors.lastname: Urbanus
authors.forename: Guri
authors.initials: G
authors.lastname: Giaever
authors.forename: Corey
authors.initials: C
authors.lastname: Nislow
mesh_terms: D020418:Algal Proteins / Q000378:metabolism*; D014150:Antipsychotic Agents / Q000494:pharmacology; D001499:Bayes Theorem; D016825:Chlamydomonas reinhardtii / Q000187:drug effects* / Q000502:physiology; D056084:Genetic Fitness; D057166:High-Throughput Screening Assays / Q000379:methods*; D008954:Models, Biological; D010641:Phenotype; D054852:Small Molecule Libraries / Q000494:pharmacology*
chemical_list: D020418:Algal Proteins; D014150:Antipsychotic Agents; D054852:Small Molecule Libraries