title: Rabies virus nucleoprotein expressed in silkworm pupae at high-levels and evaluation of immune responses in mice.
abstract: Rabies is one of the most fatal zoonotic diseases in developing countries, where a safe, cheap and effective vaccine against the disease remains unaffordable. In this paper, we describe a new silkworm-baculovirus expression system to express the nucleoprotein (N) gene of rabies virus and evaluation of the immune response in BALB/c mice. A recombinant baculovirus -rBmNPV(RV-N) carrying the N gene of rabies virus Evelyn Rokitniki Abelseth (ERA) strain was constructed and the N protein expression was evaluated in Bombyx mori (BmN) cells and silkworm pupae by immunofluorescence staining, Western blots and enzyme-linked immunosorbent assay (ELISA). The immune response to vaccines was evaluated based on serum IgG antibody titers and challenge experiments. The study revealed that N protein of rabies virus can be highly expressed in silkworm baculovirus expression system and the vaccine of N antigen presents a promising approach for the prevention of rabies virus.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Lanzhou, Gansu, China.
authors.forename: Xiangping
authors.initials: X
authors.lastname: Yin
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Li
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Li
authors.forename: Yongzhu
authors.initials: Y
authors.lastname: Yi
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xuerui
authors.initials: X
authors.lastname: Li
authors.forename: Baoyu
authors.initials: B
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.forename: Xi
authors.initials: X
authors.lastname: Lan
authors.forename: Yinv
authors.initials: Y
authors.lastname: Li
authors.forename: Wenqiang
authors.initials: W
authors.lastname: Jiao
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Jixing
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics / Q000276:immunology; D012831:Bombyx / Q000235:genetics* / Q000276:immunology; D002460:Cell Line; D005260:Female; D005455:Fluorescent Antibody Technique / Q000379:methods; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009698:Nucleoproteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology*; D011679:Pupa / Q000276:immunology; D011818:Rabies / Q000276:immunology / Q000517:prevention & control; D011820:Rabies virus / Q000235:genetics* / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D009698:Nucleoproteins; D011993:Recombinant Fusion Proteins