title: Comparative evaluation of the Nanosphere Verigene RV+ assay and the Simplexa Flu A/B & RSV kit for detection of influenza and respiratory syncytial viruses.
abstract: Using retrospective (n = 200) and prospective (n = 150) nasopharyngeal specimens, we evaluated the Nanosphere Verigene RV+ and the Focus Diagnostics Simplexa Flu A/B & RSV tests. Overall, RV+ demonstrated sensitivities and specificities of 96.6% and 100% for influenza A virus, 100% and 99.7% for influenza B virus, and 100% and 100% for respiratory syncytial virus (RSV), while the Simplexa test sensitivities and specificities were 82.8 and 99.7%, 76.2 and 100%, and 94.6 and 100%, respectively.
authors.affiliation: Clinical Microbiology/Immunology Laboratories, UNC Health Care, Chapel Hill, North Carolina, USA.
authors.forename: Kevin
authors.initials: K
authors.lastname: Alby
authors.forename: Elena B
authors.initials: EB
authors.lastname: Popowitch
authors.forename: Melissa B
authors.initials: MB
authors.lastname: Miller
mesh_terms: D019411:Clinical Laboratory Techniques / Q000379:methods*; D006801:Humans; D009980:Influenza A virus / Q000302:isolation & purification*; D009981:Influenza B virus / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology; D009305:Nasopharynx / Q000821:virology; D011446:Prospective Studies; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis* / Q000821:virology; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification*; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*