title: New insights into replisome fluidity during chromosome replication.
abstract: Several paradigm shifting advances have recently been made on the composition and function of the chromosomal DNA replication machinery. Replisomes appear to be more fluid and dynamic than ever imagined, enabling rapid and efficient bypass of roadblocks and template lesions while faithfully replicating chromosomal DNA. This fluidity is determined by many layers of regulation, which reach beyond the role of replisome components themselves. In fact, recent studies show that additional polymerases, post-transcriptional modifications, and chromatin structure are required for complete chromosome duplication. Many of these factors are involved with the more complex events that take place during lagging-strand synthesis. These, and other recent discoveries, are the focus of this review.
authors.affiliation: Rockefeller University and Howard Hughes Medical Institute, 1230 York Avenue, New York, NY 10065, USA.
authors.forename: Isabel
authors.initials: I
authors.lastname: Kurth
authors.forename: Mike
authors.initials: M
authors.lastname: O'Donnell
mesh_terms: D042002:Chromatin Assembly and Disassembly; D002876:Chromosomes, Bacterial / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000235:genetics; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005057:Eukaryotic Cells / Q000166:cytology / Q000378:metabolism; D009707:Nucleosomes / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D018741:Replication Origin
chemical_list: D004269:DNA, Bacterial; D009707:Nucleosomes; C022262:Okazaki fragments; D004247:DNA; D004259:DNA-Directed DNA Polymerase