title: Micropropagation of Cordyline terminalis.
abstract: This protocol describes an efficient and rapid method for large-scale multiplication of Cordyline terminalis in a cost-effective manner. Actively growing shoot tips were selected as explants. Murashige and Skoog (MS) basal medium was supplemented with different plant growth regulators at various developmental stages of C. terminalis. The highest percentage of regeneration (95 ± 2.8) and average number of shoot buds (60.2 ± 4.4) per explant were obtained in medium containing 80 mg /L adenine sulfate (AdSO(4)), 2 mg/L 6-benzyladenine (BA), and 0.1 mg/L indole-3-acetic acid (IAA). Thousands of micropropagated plants were produced within 4-5 months using this protocol.
authors.affiliation: Department of Botany, Centre of Advanced Study, University of Calcutta, Kolkata, India.
authors.forename: Tui
authors.initials: T
authors.lastname: Ray
authors.forename: Prasenjit
authors.initials: P
authors.lastname: Saha
authors.forename: Satyesh C
authors.initials: SC
authors.lastname: Roy
mesh_terms: D000064:Acclimatization; D031406:Cordyline / Q000166:cytology / Q000254:growth & development* / Q000502:physiology; D003470:Culture Media / Q000737:chemistry; D046508:Culture Techniques / Q000379:methods* / Q000592:standards; D018517:Plant Roots / Q000254:growth & development / Q000502:physiology; D018520:Plant Shoots / Q000254:growth & development / Q000502:physiology; D012015:Reference Standards; D012038:Regeneration; D013242:Sterilization; D013997:Time Factors
chemical_list: D003470:Culture Media