title: [The incompatibility mechanism based on the interaction of multiple-components for Flos Genkwa and Radix et Rhizoma Glycyrrhizae].
abstract: By investigating the interaction between components from Flos Genkwa (FG) and Radix et Rhizoma Glycyrrhizae (RRG) and the dissolution profile of toxic components in co-decoction, the characteristics and possible mechanism of incompatibility were revealed. Ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) and ultra-high performance liquid chromatography triple-quadrupole mass spectrometry (UPLC-TQ/MS) were used to analyze multi-components in different herb extractions prepared by different ratios of FG/FG processed by vinegar (FGV) and RRG, which reflect the interaction and characteristics of multiple components in incompatibility combinations. The results showed that the components dissolution was influenced by compatibility ratio with certain regularity. Whether FG processed by vinegar or not, with the increase of RRG in co-decoction, the dissolution of diterpenes, especially for yuanhuacine, yuanhuadine and yuanhuajine, the toxic ingredients of FG, increased significantly. From these results, the material basis and one possible mechanism of incompatibility between FG and RRG is the increasing dissolution of diterpenes, toxic components of FG in co-decoction process, which caused by interaction between multi-components in these two herbs.
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China.
authors.forename: Yan-yan
authors.initials: YY
authors.lastname: Chen
authors.forename: Da-wei
authors.initials: DW
authors.lastname: Qian
authors.forename: Er-xin
authors.initials: EX
authors.lastname: Shang
authors.forename: Ye-fei
authors.initials: YF
authors.lastname: Qian
authors.forename: Pei
authors.initials: P
authors.lastname: Liu
authors.forename: Shu-lan
authors.initials: SL
authors.lastname: Su
authors.forename: Jian-ming
authors.initials: JM
authors.lastname: Guo
authors.forename: Yu-ping
authors.initials: YP
authors.lastname: Tang
authors.forename: Jin-ao
authors.initials: JA
authors.lastname: Duan
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D032364:Daphne / Q000737:chemistry*; D004224:Diterpenes / Q000032:analysis*; D004344:Drug Incompatibility; D035264:Flowers / Q000737:chemistry; D029882:Glycyrrhiza uralensis / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D027343:Rhizome / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D013729:Terpenes / Q000032:analysis
chemical_list: D004224:Diterpenes; D013729:Terpenes; C512329:Yuanhuajine; C029912:Yuanhuacin; C039652:yuanhuadine; D019342:Acetic Acid