title: In vitro propagation of ash (Fraxinus excelsior L.) by somatic embryogenesis.
abstract: Induction of somatic embryogenesis is described in common ash (Fraxinus excelsior L.). Embryogenic tissues are obtained from immature zygotic embryos and cultured on a modified Murashige and Skoog (MS) medium containing 8.8 μM 2,4-dichlorophenoxyacetic acid and 4.4 μM benzyl-adenine. Embryogenic tissue is subcultured and multiplied on medium supplemented with reduced concentration of plant growth hormones. Somatic embryos develop and mature by transfer to hormone-free medium and subsequent culture on medium containing low amount of benzyladenine. Somatic embryo germination and conversion are enhanced by cold storage at 4°C and successive transfer onto Woody Plant Medium (WPM). Fully developed plantlets are then transferred to pots and acclimatized in the greenhouse equipped with a mist system.
authors.affiliation: IGV-Istituto di Genetica Vegetale, National Research Council (CNR), Sesto Fiorentino, Florence, Italy. maurizio.capuana@igv.cnr.it
authors.forename: Maurizio
authors.initials: M
authors.lastname: Capuana
mesh_terms: D000064:Acclimatization; D003470:Culture Media / Q000737:chemistry; D031661:Fraxinus / Q000166:cytology / Q000254:growth & development* / Q000502:physiology; D018525:Germination; D058439:Plant Somatic Embryogenesis Techniques / Q000379:methods*; D012639:Seeds / Q000254:growth & development / Q000502:physiology; D013242:Sterilization
chemical_list: D003470:Culture Media