title: Lhx8 promote differentiation of hippocampal neural stem/progenitor cells into cholinergic neurons in vitro.
abstract: Lhx8, also named L3, is a recently identified member of the LIM homeobox gene family. Previously, we found acetylcholinesterase (AChE)-positive cells in fimbria-fornix (FF) transected rat hippocampal subgranular zone (SGZ). In the present study, we detected choline acetyltransferase (ChAT)-positive cholinergic cells in hippocampal SGZ after FF transaction, and these ChAT-positive cells were double labeled by Lhx8. Then we overexpressed Lhx8 during neural differentiation of hippocampal neural stem/progenitor cells on adherent conditions using lentivirus Lenti6.3-Lhx8. The result indicated that overexpression of Lhx8 did not affect the proportion of MAP2-positive neurons, but increased the proportion of ChAT-positive cells in vitro. These results suggested that FF-transected hippocampal niche promoted the ChAT/Lhx8-positive cholinergic neurons generation in rodent hippocampus, and Lhx8 was not associated with the MAP2-positive neurons differentiation on adherent conditions, but played a role in the specification of cholinergic neurons derived from hippocampal neural stem/progenitor cells in vitro.
authors.affiliation: Department of Anatomy and Cytoneurobiology, Medical College of Soochow University, Suzhou, 215123, China.
authors.forename: Jinhong
authors.initials: J
authors.lastname: Shi
authors.forename: Haoming
authors.initials: H
authors.lastname: Li
authors.forename: Guohua
authors.initials: G
authors.lastname: Jin
authors.forename: Peipei
authors.initials: P
authors.lastname: Zhu
authors.forename: Meiling
authors.initials: M
authors.lastname: Tian
authors.forename: Jianbing
authors.initials: J
authors.lastname: Qin
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Tan
authors.forename: Shuqing
authors.initials: S
authors.lastname: Zhao
authors.forename: Fuyu
authors.initials: F
authors.lastname: Wang
authors.forename: Yurong
authors.initials: Y
authors.lastname: Hua
authors.forename: Yong
authors.initials: Y
authors.lastname: Xiao
mesh_terms: D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000502:physiology*; D017931:DNA Primers; D006624:Hippocampus / Q000166:cytology*; D066298:In Vitro Techniques; D060850:LIM-Homeodomain Proteins / Q000502:physiology*; D058953:Neural Stem Cells / Q000502:physiology*; D009474:Neurons / Q000166:cytology*; D051381:Rats; D011950:Receptors, Cholinergic / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology*
chemical_list: D017931:DNA Primers; C112960:LIM homeobox protein 8; D060850:LIM-Homeodomain Proteins; D011950:Receptors, Cholinergic; D014157:Transcription Factors