title: [Effects of secondary structure of the leader peptide on modification and processing of bovicin HJ50].
abstract: OBJECTIVE
  Elucidating the correlation between the secondary structure of the leader peptide of lantibiotic bovicin HJ50 and its modification and processing.
  
  
  METHODS
  The variants with mutated leader peptide were synthesized by semi-in vitro biosynthesis, and their modification pattern were then analyzed by MALDI-TOF MS. At the same time, the effect of leader peptide mutants on processing the modified propeptide was examined by HPLC and antimicrobial activity.
  
  
  RESULTS
  We constructed 6 mutants (F-16A, V-15E, E-14L, E-8P, L-7D, L-4K) involved in forming secondary structure of the bovicin HJ50 leader peptide. F-16A, V-15E, L-4K showed very little effect on modification and processing whereas E-14L and E-8P caused changes in modification. In addition, we found that L-7D strongly affected the processing.
  
  
  CONCLUSIONS
  The conserved helix structure in the leader peptide of bovicin HJ50 was closely related to the activity of BovM and BovT150, and the presence of secondary structure was very important to modification and processing of bovicin HJ50.
authors.affiliation: School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. zwust05@163.com
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Lingling
authors.initials: L
authors.lastname: Tu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yuheng
authors.initials: Y
authors.lastname: Lin
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Jin
authors.initials: J
authors.lastname: Zhong
mesh_terms: D000595:Amino Acid Sequence; D001430:Bacteriocins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D016985:Streptococcus bovis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D001430:Bacteriocins; D021382:Protein Sorting Signals