title: Functional characterization of three mouse formyl peptide receptors.
abstract: The evolutionary relationship and functional correlation between human formyl peptide receptors (FPRs) and their mouse counterparts remain incompletely understood. We examined three members of the mouse formyl peptide receptor subfamily (mFprs) and found that they differ in agonist preference and cellular distributions. When stably expressed in transfected rat basophilic leukemia (RBL-2H3) cells, mFpr1 was readily activated by N-formylated peptides derived from Listeria monocytogenes (fMIVTLF), Staphylococcus aureus (fMIFL), and mitochondria (fMMYALF). In contrast, the Escherichia coli-derived fMLF was 1000-fold less potent. The aforementioned peptides were much less efficacious at mFpr2, which responded better to the synthetic hexapeptide WKYMVm, the synthetic agonists Quin-C1 (a substituted quinazolinone), and compound 43 (a nitrosylated pyrazolone derivative). Saturation binding assays showed that mFpr1 and mFpr2 were expressed at similar levels on the cell surface, although their affinity for N-formyl-Met-Leu-Phe-Ile-Ile-Lys-fluorescein isothiocyanate varied by more than 1000-fold [dissociation constant (K(d)) values of 2.8 nM for mFpr1 and 4.8 μM for mFpr2]). Contrary to these receptors, mFpr-rs1 responded poorly to all the previously mentioned peptides that were tested. Fluorescent microscopy revealed an intracellular distribution pattern of mFpr-rs1. On the basis of these results, we conclude that mFpr1 is an ortholog of human FPR1 with certain pharmacologic properties of human FPR2/ALX, whereas mFpr2 has much lower affinity for formyl peptides. The intracellular distribution of mFpr-rs1 suggests an evolutionary correlation with human FPR3.
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, PR China.
authors.forename: Hui-Qiong
authors.initials: HQ
authors.lastname: He
authors.forename: Dan
authors.initials: D
authors.lastname: Liao
authors.forename: Zhen-Guo
authors.initials: ZG
authors.lastname: Wang
authors.forename: Zhong-Li
authors.initials: ZL
authors.lastname: Wang
authors.forename: Hu-Chen
authors.initials: HC
authors.lastname: Zhou
authors.forename: Ming-Wei
authors.initials: MW
authors.lastname: Wang
authors.forename: Richard D
authors.initials: RD
authors.lastname: Ye
mesh_terms: D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D045744:Cell Line, Tumor; D004926:Escherichia coli / Q000378:metabolism; D015471:Leukemia, Basophilic, Acute / Q000378:metabolism; D008089:Listeria monocytogenes / Q000378:metabolism; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000378:metabolism*; D009842:Oligopeptides / Q000494:pharmacology; D011485:Protein Binding; D011799:Quinazolines / Q000494:pharmacology; D051381:Rats; D044042:Receptors, Formyl Peptide / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000378:metabolism; D014162:Transfection / Q000379:methods
chemical_list: C493592:4-butoxy-N-(2-(4-methoxy-phenyl)-4-oxo-1,4-dihydro-2H-quinazolin-3-yl)-benzamide; D001549:Benzamides; C469989:Fpr-rs1 protein, mouse; C469988:Fpr1 protein, mouse; D009842:Oligopeptides; D011799:Quinazolines; D044042:Receptors, Formyl Peptide; C113617:Trp-Lys-Tyr-Met-Val-Met; C481578:formyl peptide receptor 2, mouse; D009240:N-Formylmethionine Leucyl-Phenylalanine; D002118:Calcium