title: Fabrication, characterization and in vitro profile based interaction with eukaryotic and prokaryotic cells of alginate-chitosan-silica biocomposite.
abstract: This work is focused on the fabrication of a new drug delivery system based on polyanionic matrix (e.g. sodium alginate), polycationic matrix (e.g. chitosan) and silica network. The FT-IR, SEM, DTA-TG, eukaryotic cell cycle and viability, and in vitro assay of the influence of the biocomposite on the efficacy of antibiotic drugs were investigated. The obtained results demonstrated the biocompatibility and the ability of the fabricated biocomposite to maintain or improve the efficacy of the following antibiotics: piperacillin-tazobactam, cefepime, piperacillin, imipenem, gentamicin, ceftazidime against Pseudomonas aeruginosa ATCC 27853 and cefazolin, cefaclor, cefuroxime, ceftriaxone, cefoxitin, trimethoprim/sulfamethoxazole against Escherichia coli ATCC 25922 reference strains.
authors.affiliation: University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Organic Chemistry Department, Polizu Street No. 1-7, 011061 Bucharest, Romania.
authors.forename: Paul Catalin
authors.initials: PC
authors.lastname: Balaure
authors.forename: Ecaterina
authors.initials: E
authors.lastname: Andronescu
authors.forename: Alexandru Mihai
authors.initials: AM
authors.lastname: Grumezescu
authors.forename: Anton
authors.initials: A
authors.lastname: Ficai
authors.forename: Keng-Shiang
authors.initials: KS
authors.lastname: Huang
authors.forename: Chih-Hui
authors.initials: CH
authors.lastname: Yang
authors.forename: Carmen Mariana
authors.initials: CM
authors.lastname: Chifiriuc
authors.forename: Yung-Sheng
authors.initials: YS
authors.lastname: Lin
mesh_terms: D000464:Alginates / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D004926:Escherichia coli / Q000187:drug effects; D005057:Eukaryotic Cells / Q000187:drug effects / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D011387:Prokaryotic Cells / Q000187:drug effects / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012822:Silicon Dioxide / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry
chemical_list: D000464:Alginates; D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D006603:Hexuronic Acids; D012822:Silicon Dioxide; D020723:Glucuronic Acid; D048271:Chitosan