title: Electroporation: past, present and future.
abstract: Gene transfer by electroporation has become an indispensable method for the study of developmental biology. The technique is applied not only in chick embryos but also in mice and other organisms. Here, a short history and perspectives of electroporation for gene transfer in vertebrates are described.
authors.affiliation: Department of Molecular Neurobiology, Graduate School of Life Sciences and Institute of Development, Aging and Cancer, Tohoku University, Seiryo-machi 4-1, Aoba-ku, Sendai, Japan. nakamura@idac.tohoku.ac.jp
authors.forename: Harukazu
authors.initials: H
authors.lastname: Nakamura
authors.forename: Junichi
authors.initials: J
authors.lastname: Funahashi
mesh_terms: D000818:Animals; D002642:Chick Embryo; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015509:Developmental Biology / Q000379:methods; D018274:Electroporation / Q000379:methods*; D018014:Gene Transfer Techniques; D005796:Genes; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D051379:Mice; D012330:RNA, Double-Stranded / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D015203:Reproducibility of Results; D019170:Somites / Q000378:metabolism; D014162:Transfection
chemical_list: D012330:RNA, Double-Stranded; D034741:RNA, Small Interfering; D004247:DNA