title: Selection of DNA aptamers with affinity for pro-gastrin-releasing peptide (proGRP), a tumor marker for small cell lung cancer.
abstract: Aptamers are single-strand oligonucleotides that are generated by the systemic evolution of ligands by exponential enrichment (SELEX) technique and that can bind to target molecules specifically. However, only a few aptamers have been developed to date against tumor markers. To utilize aptamers for tumor diagnosis, a variety of aptamers are required. Here, a single-stranded DNA aptamer specific for pro-gastrin-releasing peptide (proGRP), a marker for small cell lung cancer, was selected using SELEX. After selection, identical sequences were found in the DNA library. This sequence was selected and its binding affinity to proGRP was evaluated using surface plasmon resonance.
authors.affiliation: Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, Kanagawa, Japan.
authors.forename: Masayasu
authors.initials: M
authors.lastname: Mie
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Kai
authors.forename: Thao
authors.initials: T
authors.lastname: Le
authors.forename: Anthony E G
authors.initials: AE
authors.lastname: Cass
authors.forename: Eiry
authors.initials: E
authors.lastname: Kobatake
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D014408:Biomarkers, Tumor / Q000737:chemistry* / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000737:chemistry; D019886:Gastrin-Releasing Peptide / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D052156:SELEX Aptamer Technique; D055752:Small Cell Lung Carcinoma / Q000175:diagnosis* / Q000378:metabolism; D020349:Surface Plasmon Resonance
chemical_list: D052157:Aptamers, Nucleotide; D014408:Biomarkers, Tumor; D004277:DNA, Single-Stranded; D019886:Gastrin-Releasing Peptide