title: Cell cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells.
abstract: Transcription elongation is stimulated by positive transcription elongation factor b (P-TEFb), for which activity is repressed in the 7SK small nuclear ribonucleoprotein (7SK snRNP) complex. We show here a critical role of 7SK snRNP in growth control of primordial germ cells (PGCs). The expression of p15(INK4b), a cyclin-dependent kinase inhibitor (CDKI) gene, in PGCs is selectively activated by P-TEFb and its recruiting molecule, Brd4, when the amount of active P-TEFb is increased due to reduction of the 7SK snRNP, and PGCs consequently undergo growth arrest. These results indicate that CDKI gene-specific control of transcription by 7SK snRNP plays a pivotal role in the maintenance of PGC proliferation.
authors.affiliation: Cell Resource Center for Biomedical Research, Institute of Development, Aging, and Cancer, Tohoku University, Sendai, Miyagi, Japan.
authors.forename: Daiji
authors.initials: D
authors.lastname: Okamura
authors.forename: Ikuma
authors.initials: I
authors.lastname: Maeda
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Taniguchi
authors.forename: Yuko
authors.initials: Y
authors.lastname: Tokitake
authors.forename: Makiko
authors.initials: M
authors.lastname: Ikeda
authors.forename: Keiko
authors.initials: K
authors.lastname: Ozato
authors.forename: Nathan
authors.initials: N
authors.lastname: Mise
authors.forename: Kuniya
authors.initials: K
authors.lastname: Abe
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Noce
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Izpisua Belmonte
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Matsui
mesh_terms: D000818:Animals; D002453:Cell Cycle; D049109:Cell Proliferation; D005260:Female; D018507:Gene Expression Regulation, Developmental; D018816:Genes, cdc / Q000235:genetics*; D005854:Germ Cells / Q000166:cytology* / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D009687:Nuclear Proteins / Q000378:metabolism; D042925:Positive Transcriptional Elongation Factor B / Q000378:metabolism; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism; D017411:Ribonucleoproteins, Small Nuclear / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D000092468:Bromodomain Containing Proteins
chemical_list: D009687:Nuclear Proteins; D042925:Positive Transcriptional Elongation Factor B; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; D014157:Transcription Factors; C486694:Brd4 protein, mouse; D000092468:Bromodomain Containing Proteins