title: Genomic characterization of echovirus 6 causing aseptic meningitis in Hokkaido, Japan: a novel cluster in the nonstructural protein coding region of human enterovirus B.
abstract: We determined four complete nucleotide sequences of echovirus 6 (E6) isolated from an epidemic of aseptic meningitis (AM) in Hokkaido, Japan, in 2011. Phylogenetic analysis of the genes encoding viral capsid protein 1 revealed that the strains were closely related to E6 strains isolated in China in recent years, but they were distantly related to E6 strains isolated from patients with AM in Osaka Prefecture, Japan, in 2011. The genes encoding the viral protease and RNA-dependent RNA polymerase (3CD) were closely related to those of several non-E6 strains of the species Human enterovirus B isolated in China, South Korea, and Australia from 1999 to 2010, resulting in a novel cluster in the phylogenetic tree. These results suggest that the incidence of AM in Japan in 2011 was caused by at least two lineages of E6 strains, and a lineage of the 3CD gene was interspersed among different serotypic strains isolated in Western Pacific countries.
authors.affiliation: Center for Infectious Diseases Control, Hokkaido Institute of Public Health, North 19 West 12, Kita-ku, Sapporo, 060-0819, Japan. miyo@iph.pref.hokkaido.jp
authors.forename: Masahiro
authors.initials: M
authors.lastname: Miyoshi
authors.forename: Rika
authors.initials: R
authors.lastname: Komagome
authors.forename: Setsuko
authors.initials: S
authors.lastname: Ishida
authors.forename: Hideki
authors.initials: H
authors.lastname: Nagano
authors.forename: Kenichi
authors.initials: K
authors.lastname: Takahashi
authors.forename: Motohiko
authors.initials: M
authors.lastname: Okano
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002675:Child, Preschool; D017124:Conserved Sequence; D004458:Echovirus 6, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004457:Echovirus Infections / Q000453:epidemiology / Q000821:virology*; D016679:Genome, Viral; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008582:Meningitis, Aseptic / Q000453:epidemiology / Q000821:virology*; D008587:Meningitis, Viral / Q000453:epidemiology / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D017361:Viral Nonstructural Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D017361:Viral Nonstructural Proteins