title: Glycyrrhiza uralensis flavonoids present in anti-asthma formula, ASHMI™, inhibit memory Th2 responses in vitro and in vivo.
abstract: Allergic asthma is associated with Th2-mediated inflammation. Several flavonoids were isolated from Glycyrrhiza uralensis, one of the herbs in the anti-asthma herbal medicine intervention. The aim of this investigation was to determine whether Glycyrrhiza uralensis flavonoids have inhibitory effects on memory Th2 responses in vitro and antigen-induced Th2 inflammation in vivo. The effects of three Glycyrrhiza uralensis flavonoids on effector memory Th2 cells, D10.G4.1 (D10 cells), were determined by measuring Th2 cytokine production. Isoliquiritigenin, 7, 4'-dihydroxyflavone (7, 4'-DHF) and liquiritigenin significantly suppressed IL-4 and IL-5 production in a dose-dependent manner, 7, 4'-DHF being most potent. It was also evaluated for effects on D10 cell proliferation, GATA-3 expression and IL-4 mRNA expression, which were suppressed, with no loss of cell viability. Chronic treatment with 7, 4'-DHF in a murine model of allergic asthma not only significantly reduced eosinophilic pulmonary inflammation, serum IgE levels, IL-4 and IL-13 levels, but also increased IFN-γ production in lung cell cultures in response to antigen stimulation.
authors.affiliation: Department of Pediatrics, Mount Sinai School of Medicine, New York, NY, USA.
authors.forename: Nan
authors.initials: N
authors.lastname: Yang
authors.forename: Sangita
authors.initials: S
authors.lastname: Patil
authors.forename: Jian
authors.initials: J
authors.lastname: Zhuge
authors.forename: Ming-Chun
authors.initials: MC
authors.lastname: Wen
authors.forename: Jayaprakasam
authors.initials: J
authors.lastname: Bolleddula
authors.forename: Srinivasulu
authors.initials: S
authors.lastname: Doddaga
authors.forename: Joseph
authors.initials: J
authors.lastname: Goldfarb
authors.forename: Hugh A
authors.initials: HA
authors.lastname: Sampson
authors.forename: Xiu-Min
authors.initials: XM
authors.lastname: Li
mesh_terms: D000818:Animals; D001249:Asthma / Q000188:drug therapy* / Q000276:immunology; D002460:Cell Line; D047188:Chalcones / Q000494:pharmacology; D004195:Disease Models, Animal; D005260:Female; D044950:Flavanones / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology*; D050990:GATA3 Transcription Factor / Q000378:metabolism; D029882:Glycyrrhiza uralensis / Q000737:chemistry*; D006801:Humans; D007156:Immunologic Memory / Q000187:drug effects; D007371:Interferon-gamma / Q000276:immunology; D015847:Interleukin-4; D015848:Interleukin-5 / Q000276:immunology; D008168:Lung / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D008517:Phytotherapy; D010946:Plants, Medicinal / Q000737:chemistry; D018418:Th2 Cells / Q000187:drug effects* / Q000276:immunology
chemical_list: D047188:Chalcones; D044950:Flavanones; D005419:Flavonoids; D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; D015848:Interleukin-5; D015847:Interleukin-4; C085172:4',7-dihydroxyflavone; D007371:Interferon-gamma; C040920:isoliquiritigenin; C083152:liquiritigenin