title: An HPLC method for the determination of nifekalant hydrochloride in canine plasma and its application to a pharmacokinetic study.
abstract: In this study, a simple and selective high-performance liquid chromatography method was developed and validated for the determination of nifekalant hydrochloride in canine plasma. Liquid-liquid extraction was used to separate nifekalant hydrochloride from canine plasma and the mean extraction recoveries of nifekalant hydrochloride and the internal standard were 82.31 and 94.81%, respectively. The chromatographic separation was performed on a Dikma Diamonsil column with a mobile phase consisting of acetonitrile-20mM phosphate buffer (pH 6.2; 30:70, v/v) with flow rate of 1.0 mL/min. The standard curve was linear over the concentration range of 20-10,000 ng/mL (r(2) > 0.99). The intra-batch and inter-batch accuracy for nifekalant hydrochloride at four concentrations were 93.14-100.47% and 96.12-103.77%, respectively. The relative standard deviations were less than 15%. The method was successfully applied to a pharmacokinetic study after the intravenous administration of nifekalant hydrochloride to beagle dogs.
authors.affiliation: Key Laboratory of Drug Targeting and Drug Delivery System, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan 610041, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xiu-Mei
authors.initials: XM
authors.lastname: Liu
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Xue-Hua
authors.initials: XH
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D005260:Female; D016018:Least-Squares Analysis; D059625:Liquid-Liquid Extraction; D008297:Male; D011744:Pyrimidinones / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D011744:Pyrimidinones; C076259:nifekalant