title: Maturation of murine bone marrow dendritic cells induced by acidic Ginseng polysaccharides.
abstract: In this study, we report that a acidic polysaccharide (AGP) isolated from a Chinese medicinal herb, named Ginseng (Panax giseng C.A. Meyer), induces maturation of bone marrow dendritic cells (BMDCs) via concrete changes both inside and outside BMDCs. The impacts of AGP on BMDCs were assessed with use of conventional scanning electronic microscopy (SEM), transmission electronic microscopy (TEM) for morphology, flow cytometry (FCM) for key surface molecules, cytochemistry assay, FITC-dextran, bio-assay for phagocytosis and enzyme linked immunosorbent assay (ELISA) for production of cytokines. Our results elucidated that PPS promoted maturation of BMDCs via changes as reflected by the down-regulation of acid phosphatase (ACP) activity inside the BMDCs, which occurs when phagocytosis of BMDCs to antigen decreased, while antigen presentation increased upon maturation, higher expression of key surface molecules of MHC II, CD80, CD86, CD83, and CD40, and releasing higher level of cytokines IL-12 and low level of TNF-α. Our study suggest that AGP play marked immunostimulating role on the maturation of murine BMDCs through precise regulation of phagocytosis and enzyme activities inside the BMDCs.
authors.affiliation: Institute of Pathology and Pathophysiology, School of Basic Medical Science, China Medical University, No. 92, North Second Road, Heping District, Shenyang 110001, PR China.
authors.forename: Zuozhou
authors.initials: Z
authors.lastname: Wang
authors.forename: Jingjuan
authors.initials: J
authors.lastname: Meng
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Xia
authors.forename: Yiming
authors.initials: Y
authors.lastname: Meng
authors.forename: Lin
authors.initials: L
authors.lastname: Du
authors.forename: Zhenjie
authors.initials: Z
authors.lastname: Zhang
authors.forename: Enhua
authors.initials: E
authors.lastname: Wang
authors.forename: Fengping
authors.initials: F
authors.lastname: Shan
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D005434:Flow Cytometry; D018664:Interleukin-12 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D005894:Panax / Q000737:chemistry*; D010587:Phagocytosis; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000943:Antigens, Differentiation; D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D000135:Acid Phosphatase