title: Ultra-fast analysis of anatoxin-A using laser diode thermal desorption-atmospheric pressure chemical ionization-tandem mass spectrometry: validation and resolution from phenylalanine.
abstract: A novel approach for the analysis of the cyanobacterial toxin, anatoxin-a (ANA-a), in an environmentally relevant matrix, using laser diode thermal desorption-atmospheric pressure chemical ionization-tandem mass spectrometry (LDTD-APCI-MS/MS) is presented. The ultra-fast analysis time (15 s/sample) provided by the LDTD-APCI interface is strengthened by its ability to remove interference from phenylalanine (PHE), an isobaric interference in ANA-a analysis by MS/MS. Thus the LDTD-APCI interface avoids the time consuming steps of derivatization, chromatographic separation or solid-phase extraction prior to analysis. Method development and instrumental parameter optimizations were focused toward signal enhancement of ANA-a, and signal removal of a PHE interference as high as 500 μg/L. External calibration in a complex matrix gave detection and quantification limit values of 1 and 3 μg/L respectively, as well as good linearity (R(2) > 0.999) over nearly two orders of magnitude. Internal calibration with clomiphene (CLO) is possible and method performance was similar to that obtained by external calibration. This work demonstrated the utility of the LDTD-APCI source for ultra-fast detection and quantification of ANA-a in environmental aqueous matrices, and confirmed its ability to suppress the interference of PHE without sample preparation or chromatographic separation.
authors.affiliation: Department of Chemistry, Université de Montréal, Montreal, QC, Canada.
authors.forename: Pascal
authors.initials: P
authors.lastname: Lemoine
authors.forename: Audrey
authors.initials: A
authors.lastname: Roy-Lachapelle
authors.forename: Michèle
authors.initials: M
authors.lastname: Prévost
authors.forename: Patrice
authors.initials: P
authors.lastname: Tremblay
authors.forename: Morgan
authors.initials: M
authors.lastname: Solliec
authors.forename: Sébastien
authors.initials: S
authors.lastname: Sauvé
mesh_terms: D017033:Anabaena / Q000737:chemistry; D002138:Calibration; D002996:Clomiphene / Q000737:chemistry; D000087522:Cyanobacteria Toxins; D005504:Food Analysis; D059022:Food Safety; D010649:Phenylalanine / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014326:Tropanes / Q000032:analysis*
chemical_list: D000087522:Cyanobacteria Toxins; D014326:Tropanes; D002996:Clomiphene; D010649:Phenylalanine; C509783:anatoxin a