title: Universal primers for amplification of the complete mitochondrial control region in marine fish species.
abstract: Through multiple alignment analysis of mitochondrial tRNA-Thr and tRNA-Phe sequences from 161 fishes, new universal primers specially targeting the entire mitochondrial control region were designed. This new primer set successfully amplified the expected PCR products from various kinds of marine fish species, belonging to various families, and the amplified segments were confirmed to be the control region by sequencing. These primers provide a useful tool to study the control region diversity in economically important fish species, the possible mechanism of control region evolution, and the functions of the conserved motifs in the control region.
authors.affiliation: Key Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, PR China.
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Cheng
authors.forename: T J
authors.initials: TJ
authors.lastname: Xu
authors.forename: X X
authors.initials: XX
authors.lastname: Jin
authors.forename: D
authors.initials: D
authors.lastname: Tang
authors.forename: T
authors.initials: T
authors.lastname: Wei
authors.forename: Y Y
authors.initials: YY
authors.lastname: Sun
authors.forename: F Q
authors.initials: FQ
authors.lastname: Meng
authors.forename: G
authors.initials: G
authors.lastname: Shi
authors.forename: R X
authors.initials: RX
authors.lastname: Wang
mesh_terms: D000818:Animals; D059001:Aquatic Organisms; D001483:Base Sequence; D017124:Conserved Sequence; D017931:DNA Primers / Q000737:chemistry* / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D005399:Fishes / Q000235:genetics*; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012360:RNA, Transfer, Phe / Q000235:genetics*; D012363:RNA, Transfer, Thr / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D012360:RNA, Transfer, Phe; D012363:RNA, Transfer, Thr