title: Typing methods for legionella.
abstract: In this chapter we describe the methods currently used for subgrouping Legionella pneumophila and other non-pneumophila species. In the first part we describe monoclonal antibody (mAb) subgrouping, either by indirect immunofluorescence or indirect ELISA methods. These monoclonal antibodies are not commercially available but can be obtained for noncommercial purposes from one of the authors. Further, we describe pulsed-field gel electrophoresis (PFGE), amplified fragment length polymorphism (AFLP) and sequence-based typing (SBT) as well standardized and reproducible methods for genotyping. The SBT schema is currently available for L. pneumophila whereas PFGE and AFLP can be used for all Legionella species. For certain applications it might be useful to use spoligotyping to distinguish strains belonging to the same sequence type (ST).
authors.affiliation: Institute for Medical Microbiology and Hygiene, University of Technology, Dresden, Germany. Christian.Lueck@tu-dresden.de
authors.forename: Christian
authors.initials: C
authors.lastname: Lück
authors.forename: Norman K
authors.initials: NK
authors.lastname: Fry
authors.forename: Jürgen H
authors.initials: JH
authors.lastname: Helbig
authors.forename: Sophie
authors.initials: S
authors.lastname: Jarraud
authors.forename: Timothy G
authors.initials: TG
authors.lastname: Harrison
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D015373:Bacterial Typing Techniques / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D007875:Legionella / Q000145:classification*; D016952:Legionella pneumophila / Q000145:classification / Q000276:immunology; D012703:Serotyping