title: Immunosuppressive properties of mesenchymal stromal cells derived from amnion, placenta, Wharton's jelly and umbilical cord.
abstract: BACKGROUND
  The role of bone marrow-derived mesenchymal stromal cells (BM-MSC) in preventing the incidence and ameliorating the severity of graft-versus-host disease (GvHD) has recently been reported. However, as the collection of BM-MSC is an invasive procedure, more accessible sources of MSC are desirable.
  
  
  OBJECTIVE
  This study aimed to explore the alternative sources of MSC from amnion, placenta, Wharton's jelly and umbilical cord, which are usually discarded.
  
  
  METHODS
  MSC from those tissues were isolated using mechanical dissociation and enzymatic digestion. Their capacity for proliferation and differentiation, and ability to suppress alloreactive T-lymphocytes were studied and compared with those of BM-MSC.
  
  
  RESULTS
  MSC derived from amnion, placenta, Wharton's jelly and umbilical cord were similar to BM-MSC regarding the cell morphology, the immunophenotype as well as the differentiation ability. These MSC also elicited a similar degree of immunosuppression, as evidenced by the inhibition of alloreactive T-lymphocytes in the mixed lymphocyte reaction, compared with that of BM-MSC. MSC from umbilical cord and Wharton's jelly had a higher proliferative capacity, whereas those from amnion and placenta had a lower proliferative capacity compared with BM-MSC.
  
  
  CONCLUSIONS
  The results obtained from this study suggest that MSC from amnion, placenta, Wharton's jelly and umbilical cord can therefore be potentially used for substituting BM-MSC in several therapeutic applications, including the treatment of GvHD.
authors.affiliation: Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathumthani, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Manochantr
authors.forename: Y
authors.initials: Y
authors.lastname: U-pratya
authors.forename: P
authors.initials: P
authors.lastname: Kheolamai
authors.forename: S
authors.initials: S
authors.lastname: Rojphisan
authors.forename: M
authors.initials: M
authors.lastname: Chayosumrit
authors.forename: C
authors.initials: C
authors.lastname: Tantrawatpan
authors.forename: A
authors.initials: A
authors.lastname: Supokawej
authors.forename: S
authors.initials: S
authors.lastname: Issaragrisil
mesh_terms: D000650:Amnion / Q000166:cytology / Q000276:immunology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007165:Immunosuppression Therapy / Q000379:methods; D007166:Immunosuppressive Agents / Q000276:immunology* / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000276:immunology*; D010920:Placenta / Q000166:cytology / Q000276:immunology*; D011247:Pregnancy; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D014470:Umbilical Cord / Q000166:cytology / Q000276:immunology*; D059631:Wharton Jelly / Q000276:immunology*
chemical_list: D007166:Immunosuppressive Agents