title: Quantitative determination of erlotinib and O-desmethyl erlotinib in human EDTA plasma and lung tumor tissue.
abstract: BACKGROUND
  To increase knowledge about lung tumor tissue levels of erlotinib and its primary active metabolite, and about erlotinib plasma levels in intercalated dosing schedules, a sensitive and accurate method for determination of erlotinib and O-desmethyl erlotinib (OSI-420) in human plasma and lung tumor tissue has been developed.
  
  
  RESULTS
  A method with HPLC-MS/MS was validated over a linear range from 5 to 2500 ng/ml in plasma and from 5.0 to 500 ng/ml for lung tumor tissue homogenate (50-5000 ng/g for lung tumor). Calibration curves in plasma were used to quantify analytes in lung tumor tissue homogenate. Lung tumor tissue of 15 patients has been collected and analyzed with the presented method.
  
  
  CONCLUSIONS
  This method has been successfully validated and applied to determine plasma and lung tumor tissue concentrations of erlotinib and O-desmethyl erlotinib in patients with non-small-cell lung cancer.
authors.affiliation: Department of Pharmacy & Pharmacology, Slotervaart Hospital/The Netherlands Cancer Institute, Louwesweg 6, 1066 EC Amsterdam, The Netherlands. nienke.lankheet@slz.nl
authors.forename: N A G
authors.initials: NA
authors.lastname: Lankheet
authors.forename: E E
authors.initials: EE
authors.lastname: Schaake
authors.forename: H
authors.initials: H
authors.lastname: Rosing
authors.forename: J A
authors.initials: JA
authors.lastname: Burgers
authors.forename: J H M
authors.initials: JH
authors.lastname: Schellens
authors.forename: J H
authors.initials: JH
authors.lastname: Beijnen
authors.forename: A D R
authors.initials: AD
authors.lastname: Huitema
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004492:Edetic Acid / Q000097:blood* / Q000737:chemistry; D000069347:Erlotinib Hydrochloride; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000737:chemistry* / Q000473:pathology; D015394:Molecular Structure; D011799:Quinazolines / Q000097:blood* / Q000737:chemistry; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry
chemical_list: D011799:Quinazolines; D004492:Edetic Acid; D000069347:Erlotinib Hydrochloride