title: MRT letter: localization of endogenous hydrogen peroxide by modified processes of sample preparation for transmission electron microscope in Escherichia coli.
abstract: The bacterial endogenous hydrogen peroxide (H(2)O(2)) was detected cytochemically by its reaction with cerium chloride (CeCl(3)) to produce electron-dense deposits of cerium perhydroxides. The sequence of fixation and CeCl(3) staining of H(2)O(2) in the processing of transmission electron microscope (TEM) sample preparation is crucial to the localization of endogenous H(2)O(2) in Escherichia coli. In this study, results confirmed that the process that fixation simultaneously with CeCl(3) staining provided optimum effects for H(2)O(2) localization in E. coli. The modified process of TEM provides very efficient protection for H(2)O(2) localization and more accurate quantization for the H(2)O(2) accumulation in bacterial cells.
authors.affiliation: College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Rongliu
authors.initials: R
authors.lastname: Hu
authors.forename: Wenxue
authors.initials: W
authors.lastname: Zhu
authors.forename: Jinling
authors.initials: J
authors.lastname: Fan
authors.forename: Xinyue
authors.initials: X
authors.lastname: Pang
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.forename: Liping
authors.initials: L
authors.lastname: Wang
authors.forename: Lipeng
authors.initials: L
authors.lastname: Yang
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhao
authors.forename: Chenyang
authors.initials: C
authors.lastname: He
mesh_terms: D002563:Cerium / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry* / Q000648:ultrastructure*; D006861:Hydrogen Peroxide / Q000032:analysis*; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D011309:Preservation, Biological / Q000379:methods*; D013048:Specimen Handling / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D002563:Cerium; D006861:Hydrogen Peroxide; C026690:cerous chloride