title: Presphenoidal synchondrosis fusion in DBA/2J mice.
abstract: Cranial base growth plates are important centers of longitudinal growth in the skull and are responsible for the proper anterior placement of the face and the stimulation of normal cranial vault development. We report that the presphenoidal synchondrosis (PSS), a midline growth plate of the cranial base, closes in the DBA/2J mouse strain but not in other common inbred strains. We investigated the genetics of PSS closure in DBA/2J mice by evaluating F1, F1 backcross, and/or F1 intercross offspring from matings with C57BL/6J and DBA/1J mice, whose PSS remain open. We observed that PSS closure is genetically determined, but not inherited as a simple Mendelian trait. Employing a genome-wide SNP array, we identified a region on chromosome 11 in the C57BL/6J strain that affected the frequency of PSS closure in F1 backcross and F1 intercross offspring. The equivalent region in the DBA/1J strain did not affect PSS closure in F1 intercross offspring. We conclude that PSS closure in the DBA/2J strain is complex and modified by different loci when outcrossed with C57BL/6J and DBA/1J mice.
authors.affiliation: Orthopaedic Research Laboratories, Boston Children's Hospital, Boston, MA, USA.
authors.forename: Allysa
authors.initials: A
authors.lastname: Adams
authors.forename: Brandeis
authors.initials: B
authors.lastname: McBratney-Owen
authors.forename: Brittany
authors.initials: B
authors.lastname: Newby
authors.forename: Margot E
authors.initials: ME
authors.lastname: Bowen
authors.forename: Bjorn R
authors.initials: BR
authors.lastname: Olsen
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Warman
mesh_terms: D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics / Q000378:metabolism; D003433:Crosses, Genetic; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D050939:Gene Fusion; D008040:Genetic Linkage; D023281:Genomics / Q000379:methods; D005838:Genotype; D040582:Inheritance Patterns; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D018895:Microsatellite Repeats; D023421:Models, Animal; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D012886:Skull / Q000196:embryology*
chemical_list: D004247:DNA