title: Virulent avian infectious bronchitis virus, People's Republic of China.
abstract: A virulent avian infectious bronchitis virus (IBV) was isolated from 30-day-old broiler chickens that exhibited respiratory symptoms, nephropathologic lesions, and a high proportion of deaths in the People's Republic of China during 2005. The strain, designated YN, was genetically and pathologically characterized. Phylogenetic analysis showed that YN and most of the previously characterized IBV isolates found in China were phylogenetically classified into 2 main genetic clusters. The YN isolate caused severe lesions and resulted in deaths of 65% in experimental infections of 30-day-old specific-pathogen-free chickens. Tracheal and severe kidney lesions developed in all infected birds, confirming the ability of YN strain to induce both respiratory and renal disease. IBV antigens were detected by immunohistochemical analysis in the trachea, lung, kidney, and bursa, consistent with histopathologic observations, virus isolation, and reverse transcription PCR detection. We showed that YN IBV exhibits severe pathogenicity in chickens, and that similar viruses are prevalent in China.
authors.affiliation: The Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, China Agricultural University, Beijing, People’s Republic of China.
authors.forename: Jinling
authors.initials: J
authors.lastname: Feng
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Hu
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Ma
authors.forename: Qi
authors.initials: Q
authors.lastname: Yu
authors.forename: Jixun
authors.initials: J
authors.lastname: Zhao
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Liu
authors.forename: Guozhong
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens / Q000821:virology; D002681:China; D018352:Coronavirus Infections / Q000662:veterinary; D016679:Genome, Viral; D001351:Infectious bronchitis virus / Q000145:classification / Q000235:genetics / Q000472:pathogenicity*; D007668:Kidney / Q000473:pathology / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011201:Poultry Diseases / Q000401:mortality / Q000473:pathology / Q000821:virology; D016415:Sequence Alignment; D014764:Viral Proteins / Q000235:genetics; D014774:Virulence
chemical_list: D014764:Viral Proteins