title: Diffusion tensor imaging metrics in neonates-a comparison of manual region-of-interest analysis vs. tract-based spatial statistics.
abstract: BACKGROUND
  Diffusion tensor data can be analyzed using region-of-interest (ROI) analysis and tract-based spatial statistics (TBSS). There is essentially no literature validating or comparing these techniques in the neonate.
  
  
  OBJECTIVE
  The purpose of this study was to perform a direct comparison of fractional anisotropy (FA), axial diffusivity (AD) and radial diffusivity (RD) derived using manual ROI analysis and TBSS modified for use in neonates.
  
  
  METHODS
  This study was IRB-approved. Thirty-nine infants, 32-49 weeks post-conception age, underwent MRI at 3 T. FA, AD and RD of the callosal genu (CG) and splenium (CS) and posterior limbs of both internal capsules (PLIC) were determined using both techniques. Pearson correlation (r) was used to estimate the concordance of tensor metrics derived from these techniques.
  
  
  RESULTS
  The r value for FA in the CG, CS and left and right PLIC was 0.88, 0.75, 0.78 and 0.35, respectively. The r value for axial/radial diffusivity in the CG, CS and left and right PLIC was 0.62/0.72, 0.76/0.64, 0.68/0.9 and 0.3/0.72, respectively. The variable concordance results from problems with spatial correspondence of ROI masks between the native space and the FA skeleton.
  
  
  CONCLUSIONS
  Direct comparison between these methodologies shows tensor metrics varied with location and by degree, suggesting the two techniques do not provide consistently comparable results.
authors.affiliation: Department of Radiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX, USA. Youngseob.Seo@UTSouthwestern.edu
authors.forename: Youngseob
authors.initials: Y
authors.lastname: Seo
authors.forename: Zhiyue J
authors.initials: ZJ
authors.lastname: Wang
authors.forename: Gareth
authors.initials: G
authors.lastname: Ball
authors.forename: Nancy K
authors.initials: NK
authors.lastname: Rollins
mesh_terms: D016880:Anisotropy; D001921:Brain / Q000033:anatomy & histology*; D001931:Brain Mapping; D056324:Diffusion Tensor Imaging / Q000379:methods*; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007231:Infant, Newborn; D016014:Linear Models; D008297:Male; D015203:Reproducibility of Results