title: Combination of phage display and molecular grafting generates highly specific tumor-targeting miniproteins.
abstract: Frankenstein's peptide: the grafting of the binding domain from miniprotein Min-23 into the sunflower trypsin inhibitor (SFTI-I) peptide scaffold preserved its in vitro and in vivo binding specificity and proteolytic stability. The combination of these peptides was shown to be tumor-specific with a good binding affinity for delta-like ligand 4 (Dll4) protein. The use of SFTI-I as a peptide scaffold is ideal for hit-to-lead development.
authors.affiliation: DKFZ, Klinische Kooperationseinheit Nuklearmedizin, Heidelberg, Germany.
authors.forename: Frederic
authors.initials: F
authors.lastname: Zoller
authors.forename: Annette
authors.initials: A
authors.lastname: Markert
authors.forename: Philippe
authors.initials: P
authors.lastname: Barthe
authors.forename: Wenye
authors.initials: W
authors.lastname: Zhao
authors.forename: Wilko
authors.initials: W
authors.lastname: Weichert
authors.forename: Vasileios
authors.initials: V
authors.lastname: Askoxylakis
authors.forename: Annette
authors.initials: A
authors.lastname: Altmann
authors.forename: Walter
authors.initials: W
authors.lastname: Mier
authors.forename: Uwe
authors.initials: U
authors.lastname: Haberkorn
mesh_terms: D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000473:pathology; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D020349:Surface Plasmon Resonance; D014361:Trypsin Inhibitors / Q000737:chemistry / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D019151:Peptide Library; D010455:Peptides; D014361:Trypsin Inhibitors; C064045:delta protein