title: In-house UV radiation-damage-induced phasing of selenomethionine-labeled protein structures.
abstract: Selenomethionine labeling is the most common technique used in protein crystallography to derivatize recombinant proteins for experimental phasing using anomalous scattering at tunable synchrotron beamlines. Recently, it has been shown that UV radiation depletes electron density of selenium atoms of selenomethionine residues and that UV radiation-damage-induced phasing (equivalent to single isomorphous replacement) protocol can be applied to calculate experimental phases. Here we present the straightforward integration of a UV source with an in-house diffractometer. We show how this setup can extend the capabilities of a sealed tube X-ray generator and be used for experimental phasing of selenium-labeled proteins.
authors.affiliation: IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal.
authors.forename: Pedro José Barbosa
authors.initials: PJ
authors.lastname: Pereira
authors.forename: Antoine
authors.initials: A
authors.lastname: Royant
authors.forename: Santosh
authors.initials: S
authors.lastname: Panjikar
authors.forename: Daniele
authors.initials: D
authors.lastname: de Sanctis
mesh_terms: D003461:Crystallography / Q000379:methods*; D007834:Lasers; D008958:Models, Molecular; D011994:Recombinant Proteins / Q000032:analysis* / Q000737:chemistry; D012645:Selenomethionine / Q000737:chemistry*; D014466:Ultraviolet Rays; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D011994:Recombinant Proteins; D012645:Selenomethionine