title: [The mechanical wound transcriptome of three-year-old Aquilaria sinensis].
abstract: Chinese agarwood is formed in the aromatic resinous wood formed in Aquilaria sinensis (Lour.) Gilg (botanical family: Thymelaeaceae). Only when suffering stress of wound, etc, can A. sinensis produce sesquiterpenes etc. compounds of agarwood around wounds. However, little is known about how wound induced the biosynthesis pathway of sesquiterpenes. To reveal the molecular mechanism of wound-induced agarwood formation, RNA sequencing (RNA-seq) technology was used to investigate the profile of gene expression in A. sinensis treated by mechanical wounding and elucidate its functional gene. A total of 40,295 ESTs with an average read length of 305 bp were generated and 22 095 unigenes were formed by initial gene splicing. 61.6% of these unigenes (13 611) were annotated using BLAST searches against the SwissProt, KEGG, Nr and Nt databases. Twenty-six unigenes (encoding 7 enzymes) were found to be involved in sesquiterpene of agarwood biosynthesis by bioinformatic tools of Gene Ontology and KEGG. Novel genes that are potentially involved in sesquiterpenes biosynthesis were identified in A. sinensis, providing data for further sesquiterpenes biosynthesis pathway by molecular methods and the EST data establish a foundation for future studies in the molecular mechanisms of wound-induce agarwood formation in A. sinensis.
authors.affiliation: National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100093, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Zhi-hui
authors.initials: ZH
authors.lastname: Gao
authors.forename: Jian-he
authors.initials: JH
authors.lastname: Wei
authors.forename: Yan-hong
authors.initials: YH
authors.lastname: Xu
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Yun
authors.initials: Y
authors.lastname: Yang
authors.forename: Hui
authors.initials: H
authors.lastname: Meng
authors.forename: Chun
authors.initials: C
authors.lastname: Sui
authors.forename: Meng-xi
authors.initials: MX
authors.lastname: Wang
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000378:metabolism; D020224:Expressed Sequence Tags; D017343:Genes, Plant / Q000235:genetics; D010946:Plants, Medicinal / Q000737:chemistry / Q000235:genetics; D017423:Sequence Analysis, RNA; D012717:Sesquiterpenes / Q000737:chemistry / Q000378:metabolism*; D013312:Stress, Physiological / Q000235:genetics*; D029645:Thymelaeaceae / Q000737:chemistry / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics*; D014934:Wood / Q000235:genetics / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D012717:Sesquiterpenes