title: Poly (glycerol sebacate): a novel scaffold material for temporomandibular joint disc engineering.
abstract: The preponderance of temporomandibular joint (TMJ) disorders involving TMJ disc injury inspires the need to further explore tissue engineering strategies. The objective of this study was to examine the potential of poly (glycerol sebacate) (PGS), a biocompatible, biodegradable elastomer, as a porous scaffold material for the TMJ disc. Goat fibrochondrocytes were seeded on PGS at three seeding densities (25, 50, 100 million cells/mL scaffold), respectively, and cultured for 24 h, 2 weeks, and 4 weeks. The resulting histological, biochemical, and biomechanical properties were determined. Histological staining revealed an abundance of both collagen and glycosaminoglycans (GAG) throughout the high seeding density scaffolds at 4 weeks. There was also a significant increase in the cellular content in all groups over the four-week period, showing that the scaffolds promoted cell attachment and proliferation. The PGS scaffolds supported the deposition of large quantities of extracellular matrix, with differences noted between seeding density groups. At 4 weeks, the medium and high seeding density groups had significantly more collagen per scaffold (181±46 μg and 218±24 μg, respectively) than the low seeding density group (105±28 μg) (p<0.001). At 4 weeks, the medium and high seeding density groups also had a significantly higher GAG content per scaffold (702±253 μg and 773±187 μg, respectively), than the low seeding density group (324±73 μg) (p<0.001). The compression tangent modulus was significantly greater at 4 weeks than 24 h (123.6±86 kPa and 26.2±5 kPa, respectively) (seeding density groups combined) (p<0.001), with no differences between seeding groups at each time point. After 4 weeks, the tangent modulus of the low seeding density group was in a similar range of the goat TMJ disc (180±127 kPa compared to 304±141 kPa, respectively). The results show that cell seeding density and culture time do have an effect on both the biochemical and biomechanical properties of PGS scaffolds. These findings demonstrate that PGS has great potential as a scaffold material for TMJ disc engineering.
authors.affiliation: Department of Bioengineering, Center for Craniofacial Regeneration, McGowan Institute of Regenerative Medicine, School of Dental Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors.forename: Catherine K
authors.initials: CK
authors.lastname: Hagandora
authors.forename: Jin
authors.initials: J
authors.lastname: Gao
authors.forename: Yadong
authors.initials: Y
authors.lastname: Wang
authors.forename: Alejandro J
authors.initials: AJ
authors.lastname: Almarza
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena / Q000187:drug effects; D024043:Collagen Type II / Q000378:metabolism; D019245:Compressive Strength / Q000187:drug effects; D003651:Decanoates / Q000494:pharmacology*; D005260:Female; D005990:Glycerol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006025:Glycosaminoglycans / Q000378:metabolism; D006041:Goats; D011108:Polymers / Q000494:pharmacology*; D013194:Staining and Labeling; D019224:Temporomandibular Joint Disc / Q000187:drug effects*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D024043:Collagen Type II; D003651:Decanoates; D006025:Glycosaminoglycans; D011108:Polymers; C469892:poly(glycerol-sebacate); D005990:Glycerol