title: [Denaturing gradient gel electrophoresis analysis on bacterial community change in the phase II composting of Volvariella volvacea substrate].
abstract: OBJECTIVE
  To study the bacterial community change during the phase II composting of Volvariella volvacea substrate of cotton waste and to clarify the predominant bacteria at different composting stages.
  
  
  METHODS
  The 16S rDNA-DGGE (denaturing gradient gel electrophoresis) and clone strains sequencing methods were used to track th change of bacterial community during composting.
  
  
  RESULTS
  The DGGE profile shows that the diversity of bacteria community was rich and bands diversity decreased with the composting progress. The dynamic changes of predominant community and relative intensity were observed. The 23 predominant strains belong to 3 classes of Proteobacteria, Bacteroidetes and Firmicutes, 6 families of alpha, beta, gamma-Proteobacterium, Bacteroidetes, Sphingobacteria and Clostridia, 11 genera, in which 19 strains were thermophilic bacteria. The genera of Stenotrophomonas, Comamonas, Sphingobacterium and Sinorhizobium were predominant bacteria in the stages of high temperature and drop of temperature during composting.
  
  
  CONCLUSIONS
  The bacterial community structure and predominant community change dynamically during the phase II of Volvariella volvacea composting, especially in the course of entering the stage of high temperature.
authors.affiliation: College of Agronomy, Jilin Agricultural University, Changchun 130118, China. ccqjy@163.com
authors.forename: Changqing
authors.initials: C
authors.lastname: Chen
authors.forename: Jiang
authors.initials: J
authors.lastname: Yun
authors.forename: Li
authors.initials: L
authors.lastname: Meng
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D044822:Biodiversity; D058645:Denaturing Gradient Gel Electrophoresis; D008969:Molecular Sequence Data; D010802:Phylogeny; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D055367:Volvariella / Q000254:growth & development*
chemical_list: D012987:Soil