title: Grape seed extract as photochemopreventive agent against UVB-induced skin cancer.
abstract: BACKGROUND
  In the recent years, the use of natural antioxidants as photochemoprotective agents against skin damages produced by ultraviolet radiation gained considerable attention. Our goal was to show that the hydroethanolic extract obtained from red grape seeds, Burgund Mare (BM) variety could have a protective effect on keratinocytes exposed to UVB radiation.
  
  
  METHODS
  HaCaT keratinocytes were treated with BM extract 30 min. before UVB exposure. The effect was evaluated by assessing cell viability with MTT; the generation of lipid peroxides with malondialdehide (MDA) assay; DNA damage using comet assay; the quantification of DNA photolesions by ELISA and apoptosis by immunocytochemistry with AnnexinV.
  
  
  RESULTS
  After irradiation with UVB, HaCaT cells pretreated with BM showed: increased cell viability compared to those exposed to UVB only; significantly lower lipid peroxides level; the lesion scores and DNA photolesions were significantly lower and a significant reduction of the cells undergoing apoptosis.
  
  
  CONCLUSIONS
  These results recommend the use of the BM extract as photochemoprotective agent as such or in combination with sunscreens and/or other natural products with similar or complementary properties.
authors.affiliation: Department of Radiobiology and Tumor Biology, Ion Chiricuta Oncologic Institute, Cluj-Napoca, Romania. pmariaida@yahoo.com
authors.forename: Maria
authors.initials: M
authors.lastname: Perde-Schrepler
authors.forename: Gabriela
authors.initials: G
authors.lastname: Chereches
authors.forename: Ioana
authors.initials: I
authors.lastname: Brie
authors.forename: Corina
authors.initials: C
authors.lastname: Tatomir
authors.forename: Ion Dan
authors.initials: ID
authors.lastname: Postescu
authors.forename: Loredana
authors.initials: L
authors.lastname: Soran
authors.forename: Adriana
authors.initials: A
authors.lastname: Filip
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D056604:Grape Seed Extract / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000528:radiation effects*; D009381:Neoplasms, Radiation-Induced / Q000517:prevention & control*; D012878:Skin Neoplasms / Q000517:prevention & control*; D013473:Sunscreening Agents / Q000494:pharmacology*; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D056604:Grape Seed Extract; D013473:Sunscreening Agents