title: Expression of both matrix metalloproteinase-2 and its tissue inhibitor-2 in tunica media of radial artery in uremic patients.
abstract: OBJECTIVE
  To investigate the expression and clinical significance of both matrix metalloproteinase-2 (MMP-2) and its tissue inhibitor (tissue inhibitor of metalloproteinase-2 (TIMP-2)) in tunica media of radial artery in uremic patients.
  
  
  METHODS
  The modified radial arteries from 80 uremic patients during internal arteriovenous fistula surgery were selected and used as experimental specimens. The calcification of tunica media was observed by alizarin red staining, and the expression status of MMP-2, TIMP-2, osteoprotegerin (OPG), and osteopontin (OPN) in tunica media of radial arteries of these patients was detected by immunohistochemical method. The semiquantitative analysis and comparison were conducted based on the calcification grading and the expression of each test protein in tunica media of radial artery.
  
  
  RESULTS
  Of the 80 cases of radial artery specimens, 37 cases were presented with various degrees of calcification of tunica media, and the calcification rate was 46.25%; the expression of MMP-2, TIMP-2, OPG, and OPN could be detected in the calcificated tunica media of the radial artery and was positively correlated with the degree of vascular calcification (p < 0.05).
  
  
  CONCLUSIONS
  The incidence of vascular calcification in uremic patients was high. The occurrence of calcification in tunica media of the radial artery was correlated with the expression of MMP-2 and TIMP-2.
authors.affiliation: Department of Nephrology, Ningbo Yinzhou People's Hospital, Zhejiang Province, Ningbo, China.
authors.forename: Aiqin
authors.initials: A
authors.lastname: Shan
authors.forename: Canxin
authors.initials: C
authors.lastname: Zhou
authors.forename: Yongping
authors.initials: Y
authors.lastname: He
authors.forename: Weisheng
authors.initials: W
authors.lastname: Feng
authors.forename: Gun
authors.initials: G
authors.lastname: Chen
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhong
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000378:metabolism; D002114:Calcinosis / Q000201:enzymology* / Q000453:epidemiology / Q000209:etiology; D002681:China / Q000453:epidemiology; D018450:Disease Progression; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D015994:Incidence; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis*; D008875:Middle Aged; D017534:Radial Artery / Q000201:enzymology* / Q000473:pathology; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000096:biosynthesis*; D017540:Tunica Media / Q000201:enzymology* / Q000473:pathology; D014511:Uremia / Q000150:complications / Q000201:enzymology* / Q000473:pathology
chemical_list: D015415:Biomarkers; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2