title: Exploring an orbitrap analyzer for the characterization of intact antibodies by native mass spectrometry.
abstract: Antibody profiling: native mass spectrometry analysis of intact antibodies can be achieved with improved speed, sensitivity, and mass resolution by using a modified orbitrap instrument. Complex mixtures of monoclonal antibodies can be resolved and their glycan "fingerprints" can be profiled. Noncovalent interactions are maintained, thus allowing antibody-antigen binding to be measured.
authors.affiliation: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre and Utrecht Institute of Pharmaceutical Sciences, Utrecht University, The Netherlands.
authors.forename: Sara
authors.initials: S
authors.lastname: Rosati
authors.forename: Rebecca J
authors.initials: RJ
authors.lastname: Rose
authors.forename: Natalie J
authors.initials: NJ
authors.lastname: Thompson
authors.forename: Esther
authors.initials: E
authors.lastname: van Duijn
authors.forename: Eugen
authors.initials: E
authors.lastname: Damoc
authors.forename: Eduard
authors.initials: E
authors.lastname: Denisov
authors.forename: Alexander
authors.initials: A
authors.lastname: Makarov
authors.forename: Albert J R
authors.initials: AJ
authors.lastname: Heck
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D000941:Antigens / Q000276:immunology; D006031:Glycosylation; D057809:HEK293 Cells; D006801:Humans; D015850:Interleukin-6 / Q000276:immunology; D013058:Mass Spectrometry; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D015850:Interleukin-6; D011994:Recombinant Proteins