title: A monomeric red fluorescent protein with low cytotoxicity.
abstract: Multicolour labelling with fluorescent proteins is frequently used to differentially highlight specific structures in living systems. Labelling with fusion proteins is particularly demanding and is still problematic with the currently available palette of fluorescent proteins that emit in the red range due to unsuitable subcellular localization, protein-induced toxicity and low levels of labelling efficiency. Here we report a new monomeric red fluorescent protein, called FusionRed, which demonstrates both high efficiency in fusions and low toxicity in living cells and tissues.
authors.affiliation: Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
authors.forename: I I
authors.initials: II
authors.lastname: Shemiakina
authors.forename: G V
authors.initials: GV
authors.lastname: Ermakova
authors.forename: P J
authors.initials: PJ
authors.lastname: Cranfill
authors.forename: M A
authors.initials: MA
authors.lastname: Baird
authors.forename: R A
authors.initials: RA
authors.lastname: Evans
authors.forename: E A
authors.initials: EA
authors.lastname: Souslova
authors.forename: D B
authors.initials: DB
authors.lastname: Staroverov
authors.forename: A Y
authors.initials: AY
authors.lastname: Gorokhovatsky
authors.forename: E V
authors.initials: EV
authors.lastname: Putintseva
authors.forename: T V
authors.initials: TV
authors.lastname: Gorodnicheva
authors.forename: T V
authors.initials: TV
authors.lastname: Chepurnykh
authors.forename: L
authors.initials: L
authors.lastname: Strukova
authors.forename: S
authors.initials: S
authors.lastname: Lukyanov
authors.forename: A G
authors.initials: AG
authors.lastname: Zaraisky
authors.forename: M W
authors.initials: MW
authors.lastname: Davidson
authors.forename: D M
authors.initials: DM
authors.lastname: Chudakov
authors.forename: D
authors.initials: D
authors.lastname: Shcherbo
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000633:toxicity*; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D055503:Protein Multimerization / Q000187:drug effects; D011993:Recombinant Fusion Proteins / Q000633:toxicity; D014982:Xenopus laevis; D000097573:Red Fluorescent Protein
chemical_list: D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D000097573:Red Fluorescent Protein