title: Evaluation of anxiolytic activity of compound Valeriana jatamansi Jones in mice.
abstract: BACKGROUND
  Compound Valeriana jatamansi Jones is a formula for treating anxiety-related diseases in the clinic, which is composed of Valeriana jatamansi Rhizoma et Radix, Ziziphi Spinosae Semen, Albiziae Cortex and Junci Medulla. The purpose of this study was to explore the anxiolytic properties of this compound in mice.
  
  
  METHODS
  Male ICR mice were treated with compound Valerianae Jatamansi Jones (1.2 g/kg, 2.4 g/kg, 4.8 g/kg), saline, diazepam (2 mg/kg) orally for 10 days and then exposed to elevated maze-plus (EPM) and light-dark box (LDB). The effects of the compound on spontaneous activity were evaluated by locomotor activity test. We further investigated the mechanism of action underlying the anxiolytic-like effect of compound by pre-treating animals with antagonists of benzodiazepine (flumazenil, 3mg/kg) prior to evaluation using EPM and LDB.
  
  
  RESULTS
  Compound Valerianae Jatamansi Jones (2.4, 4.8 g/kg, p.o.) significantly increased entries (P<0.05) into and time spent (P<0.05) on the open arms of the EPM, and number of transitions (P<0.05) and time spent (P<0.05) in the light compartment of the LDB. However, the anxiolytic-like effects of compound were significantly reduced by pre-treatment with flumazenil (P>0.05). In addition, compound Valerianae Jatamansi Jones treatment didn't affect the spontaneous activity in mice (P> 0.05).
  
  
  CONCLUSIONS
  The present study supports the hypothesis that compound Valeriana jatamansi Jones exert anxiolytic action but no sedative effects in mice and that this effect might be mediated by benzodiazepine receptors.
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, PR China.
authors.forename: Jie-Shu
authors.initials: JS
authors.lastname: You
authors.forename: Min
authors.initials: M
authors.lastname: Peng
authors.forename: Jin-Li
authors.initials: JL
authors.lastname: Shi
authors.forename: Hu-Zhan
authors.initials: HZ
authors.lastname: Zheng
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Bao-Sheng
authors.initials: BS
authors.lastname: Zhao
authors.forename: Jian-You
authors.initials: JY
authors.lastname: Guo
mesh_terms: D031289:Albizzia; D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001007:Anxiety / Q000188:drug therapy*; D001522:Behavior, Animal / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D005442:Flumazenil / Q000494:pharmacology; D018757:GABA Modulators / Q000494:pharmacology; D008027:Light; D019684:Magnoliopsida; D008297:Male; D018782:Maze Learning; D051379:Mice; D008813:Mice, Inbred ICR; D009043:Motor Activity / Q000187:drug effects*; D008517:Phytotherapy; D011963:Receptors, GABA-A / Q000378:metabolism*; D014632:Valerian; D031957:Ziziphus
chemical_list: D014151:Anti-Anxiety Agents; D004365:Drugs, Chinese Herbal; D018757:GABA Modulators; D011963:Receptors, GABA-A; D005442:Flumazenil