title: Differential interactions of virulent and non-virulent H. parasuis strains with naïve or swine influenza virus pre-infected dendritic cells.
abstract: Pigs possess a microbiota in the upper respiratory tract that includes Haemophilus parasuis. Pigs are also considered the reservoir of influenza viruses and infection with this virus commonly results in increased impact of bacterial infections, including those by H. parasuis. However, the mechanisms involved in host innate responses towards H. parasuis and their implications in a co-infection with influenza virus are unknown. Therefore, the ability of a non-virulent H. parasuis serovar 3 (SW114) and a virulent serovar 5 (Nagasaki) strains to interact with porcine bone marrow dendritic cells (poBMDC) and their modulation in a co-infection with swine influenza virus (SwIV) H3N2 was examined. At 1 hour post infection (hpi), SW114 interaction with poBMDC was higher than that of Nagasaki, while at 8 hpi both strains showed similar levels of interaction. The co-infection with H3N2 SwIV and either SW114 or Nagasaki induced higher levels of IL-1β, TNF-α, IL-6, IL-12 and IL-10 compared to mock or H3N2 SwIV infection alone. Moreover, IL-12 and IFN-α secretion differentially increased in cells co-infected with H3N2 SwIV and Nagasaki. These results pave the way for understanding the differences in the interaction of non-virulent and virulent strains of H. parasuis with the swine immune system and their modulation in a viral co-infection.
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona (UAB), 08193 Bellaterra, Barcelona, Spain. maria.montoya@cresa.uab.es.
authors.forename: Tufária
authors.initials: T
authors.lastname: Mussá
authors.forename: Carolina
authors.initials: C
authors.lastname: Rodríguez-Cariño
authors.forename: Alejandro
authors.initials: A
authors.lastname: Sánchez-Chardi
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Baratelli
authors.forename: Mar
authors.initials: M
authors.lastname: Costa-Hurtado
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Fraile
authors.forename: Javier
authors.initials: J
authors.lastname: Domínguez
authors.forename: Virginia
authors.initials: V
authors.lastname: Aragon
authors.forename: María
authors.initials: M
authors.lastname: Montoya
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000382:microbiology / Q000821:virology; D060085:Coinfection / Q000276:immunology / Q000382:microbiology / Q000662:veterinary* / Q000821:virology; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D003713:Dendritic Cells / Q000378:metabolism / Q000382:microbiology* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006192:Haemophilus Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D044137:Haemophilus parasuis / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D053122:Influenza A Virus, H3N2 Subtype / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D011070:Poly I-C / Q000276:immunology; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D014774:Virulence
chemical_list: D016207:Cytokines; D011070:Poly I-C