title: [Effects of KIAA0101 expression on proliferation and invasion of gastric carcinoma MKN-45 cells].
abstract: OBJECTIVE
  To investigate the expression of KIAA0101 protein in gastric carcinoma cells, and to explore the effects of its down-regulation on the cell proliferation, cell cycle and invasion.
  
  
  METHODS
  Western blot was used to detect KIAA0101 protein expression in three gastric carcinoma cell lines including MKN-28, SGC-7901 and MKN-45. KIAA0101 siRNA and control siRNA were utilized to transfect MKN-45 cells, respectively. CCK-8 was used to analyze the changes of cell proliferation, and flow cytometry to examine the changes of cell cycle distribution. Finally, Boyden chamber was used to detect the ability of cell invasion.
  
  
  RESULTS
  Relative level of KIAA0101 protein in MKN-45 cells was significantly higher than those in MKN-28 and SGC-7901 cells, and there was significant difference among the three cell lines (P < 0.05). The result of CCK-8 study demonstrated that, compared with untreated group and control siRNA group, the proliferation of MKN-45 cells in KIAA0101 siRNA group was significantly inhibited (P < 0.05). Additionally, the result of cell cycle analysis revealed that the percentage of cell number in G(0)/G(1) phase in KIAA0101 siRNA group [(61.47 ± 0.89)%] was significantly higher than those in untreated group [(47.43 ± 0.85)%] and control siRNA group [(48.43 ± 0.73)%; F = 271.653, P = 0.000]. Further, Boyden chamber assay showed that the cell numbers migrated to Matrigel in KIAA0101 siRNA group (61.51 ± 4.76) were significantly lower than those in untreated group (138.74 ± 10.16) and control siRNA group (132.93 ± 11.25; F = 65.949, P = 0.000).
  
  
  CONCLUSIONS
  Down-regulation of KIAA0101 expression leads to an inhibition of cell proliferation, cell cycle and cell invasion. It may provide a novel target for the treatment of patients with gastric carcinoma.
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453100, China. zhangcaifeng666@163.com
authors.forename: Cai-feng
authors.initials: CF
authors.lastname: Zhang
authors.forename: Yong-hua
authors.initials: YH
authors.lastname: Xia
authors.forename: Qing-fen
authors.initials: QF
authors.lastname: Zheng
authors.forename: Zhen-juan
authors.initials: ZJ
authors.lastname: Li
authors.forename: Xiao-he
authors.initials: XH
authors.lastname: Guo
authors.forename: Hui-cong
authors.initials: HC
authors.lastname: Zhou
authors.forename: Li-li
authors.initials: LL
authors.lastname: Zhang
authors.forename: Liang-peng
authors.initials: LP
authors.lastname: Dong
authors.forename: Yu
authors.initials: Y
authors.lastname: Han
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D004268:DNA-Binding Proteins; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D014162:Transfection
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C425208:PCLAF protein, human; D034741:RNA, Small Interfering