title: Integrated bioinformatic and targeted deletion analyses of the SRS gene superfamily identify SRS29C as a negative regulator of Toxoplasma virulence.
abstract: The Toxoplasma gondii SRS gene superfamily is structurally related to SRS29B (formerly SAG1), a surface adhesin that binds host cells and stimulates host immunity. Comparative genomic analyses of three Toxoplasma strains identified 182 SRS genes distributed across 14 chromosomes at 57 genomic loci. Eight distinct SRS subfamilies were resolved. A core 69 functional gene orthologs were identified, and strain-specific expansions and pseudogenization were common. Gene expression profiling demonstrated differential expression of SRS genes in a developmental-stage- and strain-specific fashion and identified nine SRS genes as priority targets for gene deletion among the tissue-encysting coccidia. A Δsag1 sag2A mutant was significantly attenuated in murine acute virulence and showed upregulated SRS29C (formerly SRS2) expression. Transgenic overexpression of SRS29C in the virulent RH parent was similarly attenuated. Together, these findings reveal SRS29C to be an important regulator of acute virulence in mice and demonstrate the power of integrated genomic analysis to guide experimental investigations.
  
  
  OBJECTIVE
  Parasitic species employ large gene families to subvert host immunity to enable pathogen colonization and cause disease. Toxoplasma gondii contains a large surface coat gene superfamily that encodes adhesins and virulence factors that facilitate infection in susceptible hosts. We generated an integrated bioinformatic resource to predict which genes from within this 182-gene superfamily of adhesin-encoding genes play an essential role in the host-pathogen interaction. Targeted gene deletion experiments with predicted candidate surface antigens identified SRS29C as an important negative regulator of acute virulence in murine models of Toxoplasma infection. Our integrated computational and experimental approach provides a comprehensive framework, or road map, for the assembly and discovery of additional key pathogenesis genes contained within other large surface coat gene superfamilies from a broad array of eukaryotic pathogens.
authors.affiliation: Program in Molecular Structure and Function, Hospital for Sick Children, and Department of Biochemistry & Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.
authors.forename: James D
authors.initials: JD
authors.lastname: Wasmuth
authors.forename: Viviana
authors.initials: V
authors.lastname: Pszenny
authors.forename: Simon
authors.initials: S
authors.lastname: Haile
authors.forename: Emily M
authors.initials: EM
authors.lastname: Jansen
authors.forename: Alexandra T
authors.initials: AT
authors.lastname: Gast
authors.forename: Alan
authors.initials: A
authors.lastname: Sher
authors.forename: Jon P
authors.initials: JP
authors.lastname: Boyle
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Boulanger
authors.forename: John
authors.initials: J
authors.lastname: Parkinson
authors.forename: Michael E
authors.initials: ME
authors.lastname: Grigg
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods*; D004195:Disease Models, Animal; D005260:Female; D017353:Gene Deletion; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D051379:Mice; D015800:Protozoan Proteins / Q000235:genetics*; D017384:Sequence Deletion; D014122:Toxoplasma / Q000235:genetics* / Q000472:pathogenicity*; D014124:Toxoplasmosis, Animal / Q000469:parasitology; D014157:Transcription Factors / Q000235:genetics*; D014774:Virulence; D037521:Virulence Factors / Q000096:biosynthesis*
chemical_list: D015800:Protozoan Proteins; D014157:Transcription Factors; D037521:Virulence Factors