title: Characterization and antibacterial activity of amoxicillin-loaded electrospun nano-hydroxyapatite/poly(lactic-co-glycolic acid) composite nanofibers.
abstract: We report a facile approach to fabricating electrospun drug-loaded organic/inorganic hybrid nanofibrous system for antibacterial applications. In this study, nano-hydroxyapatite (n-HA) particles loaded with a model drug, amoxicillin (AMX) were dispersed into poly(lactic-co-glycolic acid) (PLGA) solution to form electrospun hybrid nanofibers. The loading of AMX onto n-HA surfaces (AMX/n-HA) and the formation of AMX/n-HA/PLGA composite nanofibers were characterized using different techniques. We show that AMX can be successfully adsorbed onto the n-HA surface and the formed AMX/n-HA/PLGA composite nanofibers have a uniform and smooth morphology with improved mechanical durability. Cell viability assay and cell morphology observation reveal that the formed AMX/n-HA/PLGA composite nanofibers are cytocompatible. Importantly, the loaded AMX within the n-HA/PLGA hybrid nanofibers shows a sustained release profile and a non-compromised activity to inhibit the growth of a model bacterium, Staphylococcus aureus. With the significantly reduced burst-release profile, good cytocompatibility, improved mechanical durability, as well as the remained antibacterial activity, the developed AMX/n-HA/PLGA composite nanofibers should find various potential applications in the fields of tissue engineering and pharmaceutical science.
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, People's Republic of China.
authors.forename: Fuyin
authors.initials: F
authors.lastname: Zheng
authors.forename: Shige
authors.initials: S
authors.lastname: Wang
authors.forename: Shihui
authors.initials: S
authors.lastname: Wen
authors.forename: Mingwu
authors.initials: M
authors.lastname: Shen
authors.forename: Meifang
authors.initials: M
authors.lastname: Zhu
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Shi
mesh_terms: D000658:Amoxicillin / Q000008:administration & dosage*; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D017886:Durapatite / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods; D019344:Lactic Acid / Q000737:chemistry*; D053769:Nanocapsules / Q000008:administration & dosage* / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012399:Rotation; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000502:physiology*
chemical_list: D000900:Anti-Bacterial Agents; D053769:Nanocapsules; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D000658:Amoxicillin; D017886:Durapatite