title: In-depth insight into in vivo apoplastic secretome of rice-Magnaporthe oryzae interaction.
abstract: The in vivo apoplastic fluid secretome of rice-blast fungus interaction remains largely uncharacterized. Here, we report a proteomics investigation of in vivo secreted proteins of rice leaves infected with incompatible (KJ401) and compatible (KJ301) races of Magnaporthe oryzae (M. oryzae) using 2-DGE and MudPIT coupled with MALDI-TOF-MS and/or nESI-LC-MS/MS analyses. Prepared fractions of secretory proteins were essentially free from cytoplasmic contamination. Two-DGE and MudPIT identified 732 secretory proteins, where 291 (40%) and 441 (60%) proteins were derived from rice and M. oryzae, respectively. Of these, 39.2% (rice) and 38.9% (M. oryzae) of proteins were predicted by SignalP as retaining signal peptides. Among these, rice secreted more proteins related to stress response, ROS and energy metabolism, whereas, M. oryzae secreted more proteins involved in metabolism and cell wall hydrolyses. Semi-quantitative RT-PCR revealed their differential expression under compatible/incompatible interactions. In vivo expression of M. oryzae glycosyl hydrolase (GH) protein family members using particle bombardment driven transient expression system showed that four GH genes could act as effectors within host apoplast possibly via interaction with host membrane bound receptor. The established in vivo secretome serves as a valuable resource toward secretome analysis of rice-M. oryzae interaction.
authors.affiliation: Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, 660-701, South Korea.
authors.forename: Sang Gon
authors.initials: SG
authors.lastname: Kim
authors.forename: Yiming
authors.initials: Y
authors.lastname: Wang
authors.forename: Kyung Hee
authors.initials: KH
authors.lastname: Lee
authors.forename: Zee-Yong
authors.initials: ZY
authors.lastname: Park
authors.forename: Jongsun
authors.initials: J
authors.lastname: Park
authors.forename: Jingni
authors.initials: J
authors.lastname: Wu
authors.forename: Soon Jae
authors.initials: SJ
authors.lastname: Kwon
authors.forename: Yong-Hwan
authors.initials: YH
authors.lastname: Lee
authors.forename: Ganesh Kumar
authors.initials: GK
authors.lastname: Agrawal
authors.forename: Randeep
authors.initials: R
authors.lastname: Rakwal
authors.forename: Sun Tae
authors.initials: ST
authors.lastname: Kim
authors.forename: Kyu Young
authors.initials: KY
authors.lastname: Kang
mesh_terms: D005656:Fungal Proteins / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D018506:Gene Expression Regulation, Plant; D054884:Host-Pathogen Interactions / Q000502:physiology*; D020082:Magnaporthe / Q000502:physiology*; D012275:Oryza / Q000378:metabolism / Q000382:microbiology; D018384:Oxidative Stress; D010935:Plant Diseases; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D010940:Plant Proteins; D020543:Proteome