title: Endothelial nitric oxide synthase Glu298Asp (G894T) gene polymorphism in coronary artery disease patients with type 2 diabetes mellitus.
abstract: OBJECTIVE
  Endothelial dysfunction is thought to be a significant risk factor for cardiovascular disease. This study determined the role of endothelial nitric oxide synthase (eNOS) Glu298Asp polymorphism and intergenotypic variation of plasma nitric oxide (NO) levels in coronary artery disease (CAD) patients with type 2 diabetes mellitus (DM).
  
  
  METHODS
  This case-control study included 28 documented CAD patients with type 2 DM and 32 non-diabetic patients with CAD. Fifty healthy volunteers without any major cardiovascular risk factors served as controls. NO was estimated by modified Griess method. The eNOS gene polymorphism was studied by amplifying DNA by PCR and digesting with BanII restriction enzyme. Restriction fragment length polymorphism was studied by using a gel documentation system.
  
  
  RESULTS
  The genotype frequencies for Glu298Asp (GT) genotype were 10.71% in diabetic CAD patients, 28.1% in non-diabetic CAD patients and 12% in controls. The T allele frequency was higher in the non-diabetic CAD group (14%) as compared with the diabetic CAD (5.4%) and control group (6%). NO level was significantly lower in non-diabetic CAD patients (10.25 mmol/L) but not in diabetic CAD patients (13.89 mmol/L) as compared to controls (16.78 mmol/L).
  
  
  CONCLUSIONS
  Glu298Asp polymorphism is not the mediator of increased incidence of CAD in diabetic patients.
authors.affiliation: Department of Biochemistry, Lady Hardinge Medical College and Associated Hospitals, New Delhi, India. vandanasaini2@gmail.com
authors.forename: Vandana
authors.initials: V
authors.lastname: Saini
authors.forename: M K
authors.initials: MK
authors.lastname: Bhatnagar
authors.forename: Jayashree
authors.initials: J
authors.lastname: Bhattacharjee
mesh_terms: D001224:Aspartic Acid; D050197:Atherosclerosis / Q000201:enzymology* / Q000453:epidemiology / Q000235:genetics; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology* / Q000453:epidemiology / Q000235:genetics; D003925:Diabetic Angiopathies / Q000201:enzymology* / Q000453:epidemiology / Q000235:genetics; D018450:Disease Progression; D005260:Female; D005838:Genotype; D018698:Glutamic Acid; D006801:Humans; D007194:India / Q000453:epidemiology; D008055:Lipids / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood*; D052250:Nitric Oxide Synthase Type III / Q000097:blood / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: D015415:Biomarkers; D008055:Lipids; D001224:Aspartic Acid; D009569:Nitric Oxide; D018698:Glutamic Acid; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III