title: Cell instructive microporous scaffolds through interface engineering.
abstract: The design of novel biomaterials for regenerative medicine requires incorporation of well-defined physical and chemical properties that mimic the native extracellular matrix (ECM). Here, we report the synthesis and characterization of porous foams prepared by high internal phase emulsion (HIPE) templating using amphiphilic copolymers that act as surfactants during the HIPE process. We combine different copolymers exploiting oil-water interface confined phase separation to engineer the surface topology of foam pores with nanoscopic domains of cell inert and active chemistries mimicking native matrix. We further demonstrate how proteins and hMSCs adhere in a domain specific manner.
authors.affiliation: The Krebs Institute, The University of Sheffield, Sheffield S10 2TN, United Kingdom.
authors.forename: Priyalakshmi
authors.initials: P
authors.lastname: Viswanathan
authors.forename: Somyot
authors.initials: S
authors.lastname: Chirasatitsin
authors.forename: Kamolchanok
authors.initials: K
authors.lastname: Ngamkham
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Engler
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Battaglia
mesh_terms: D000327:Adsorption; D057005:Bioengineering; D002448:Cell Adhesion; D002470:Cell Survival; D053595:Embryonic Stem Cells / Q000737:chemistry* / Q000166:cytology; D004655:Emulsions / Q000737:chemistry; D006801:Humans; D008648:Mesoderm / Q000737:chemistry* / Q000166:cytology; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D016062:Porosity; D011506:Proteins / Q000737:chemistry; D013499:Surface Properties; D013501:Surface-Active Agents / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D004655:Emulsions; D011108:Polymers; D011506:Proteins; D013501:Surface-Active Agents