title: Fish oil reverses the altered glucose transporter, phosphorylation, insulin receptor substrate-1 protein level and lipid contents in the skeletal muscle of sucrose-rich diet fed rats.
abstract: The role and underlying mechanisms by which n-3 polyunsaturated fatty acids (PUFA) prevent/reverse SRD-induced insulin resistance (IR) in the muscle are not completely understood. Therefore, we examined: triglyceride, diacylglycerol, PKCθ, Glut-4, enzymatic hexokinase activity, IRS-1 protein mass level, and fatty acid composition of muscle phospholipids. Rats were fed a SRD during 6 months. Thereafter, half the animals continued with SRD up to 8 months; the other half was fed a SRD in which CO (8% wt/wt) was replaced by FO (7%+1% CO) for 2 months. Results were compared with those obtained in rats fed a control diet (CD). In SRD-fed rats, FO oil normalized/improved lipid storage and PKCθ protein mass level. Effects of insulin were comparable with those of CD-fed rats. FO reversed impaired glucose phosphorylation, IRS-1, and, under insulin stimulation, Glut-4 protein mass level. FO normalized insulin resistance and increased n-3 PUFAs in muscle phospholipids.
authors.affiliation: Department of Biochemistry, School of Biochemistry, University of Litoral, Santa Fe 3000 Argentina.
authors.forename: María E
authors.initials: ME
authors.lastname: D'Alessandro
authors.forename: Adriana
authors.initials: A
authors.lastname: Chicco
authors.forename: Yolanda B
authors.initials: YB
authors.lastname: Lombardo
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects; D019422:Dietary Sucrose / Q000009:adverse effects*; D004075:Diglycerides / Q000378:metabolism; D050171:Dyslipidemias / Q000188:drug therapy / Q000378:metabolism; D002149:Energy Intake / Q000187:drug effects; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005395:Fish Oils / Q000627:therapeutic use*; D051246:Glucose Transport Proteins, Facilitative / Q000378:metabolism*; D055504:Insulin Receptor Substrate Proteins / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D014280:Triglycerides / Q000378:metabolism
chemical_list: D019422:Dietary Sucrose; D004075:Diglycerides; D005231:Fatty Acids, Unsaturated; D005395:Fish Oils; D051246:Glucose Transport Proteins, Facilitative; D055504:Insulin Receptor Substrate Proteins; D014280:Triglycerides