title: TLR9 is dispensable for intestinal ischemia/reperfusion-induced tissue damage.
abstract: The mortality rate due to intestinal ischemia/reperfusion (IR) remains at 60-80%. As toll-like receptor (TLR) 4 has been shown to be critical for IR injury in several organs, including the intestine, and TLR9 is necessary for IR-induced damage of the liver, we investigated the hypothesis that TLR9 is involved in intestinal IR-induced damage. Wildtype (C57Bl/6) and TLR9(-/-) mice were subjected to intestinal IR or Sham treatment. Several markers of damage and inflammation were assessed, including mucosal injury, eicosanoid production, cytokine secretion and complement deposition. Although IR-induced injury was not altered, PGE(2) production was decreased in TLR9(-/-) mice. Attenuated PGE(2) production was not due to differences in percentage of lipids or COX-2 transcription. The data indicate that TLR9 is not required for IR-induced injury or inflammation of the intestine.
authors.affiliation: Division of Biology, 18 Ackert Hall, Kansas State University, Manhattan, KS 66506, USA.
authors.forename: Emily Archer
authors.initials: EA
authors.lastname: Slone
authors.forename: Michael R
authors.initials: MR
authors.lastname: Pope
authors.forename: Mary
authors.initials: M
authors.lastname: Roth
authors.forename: Ruth
authors.initials: R
authors.lastname: Welti
authors.forename: Sherry D
authors.initials: SD
authors.lastname: Fleming