title: Hydroxylamine amplified gold nanoparticle-based aptameric system for the highly selective and sensitive detection of platelet-derived growth factor.
abstract: This study developed a sensitive and selective aptamer-based chemiluminescent (CL) method for the determination of platelet-derived growth factor (PDGF)-BB using hydroxylamine enlarged gold nanoparticles (Au NPs). Rabbit anti-human PDGF-BB polyclonal antibody was covalently coupled on the 96-well plate that offers reactive N-oxysuccinimide ester (referred to as NOS group) surface. In the presence of target protein, the biotinylated aptamer was captured on the 96-well plate forming an antibody/PDGF-BB/biotinylated aptamer sandwiched complex, which was followed by the assembly of streptavidin coated Au NPs (streptavidin-gold). Au NPs assembled on the surface of 96-well plate reacted with HAuCl(4) and NH(2)OH, which enabled the catalytic deposition of gold metal onto the Au NPs surfaces. A huge number of Au(3+) ions were released from the hydroxylamine enlarged Au NPs after oxidative gold metal dissolution, which was determined by a simple and sensitive luminol CL reaction. The results showed that the detection limit of the assay is 60 pM of PDGF-BB (corresponding to 6 fmol in a 100 μL volume), which compares favorably with those of other PDGF-BB detection techniques. In addition, this aptameric CL biosensor demonstrated extraordinary specificity. And PDGF-BB has been determined in diluted serum indicating the applicability of this assay.
authors.affiliation: Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Yinhuan
authors.initials: Y
authors.lastname: Song
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Aiping
authors.initials: A
authors.lastname: Fan
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D000077214:Becaplermin; D015374:Biosensing Techniques; D006046:Gold / Q000737:chemistry*; D006801:Humans; D019811:Hydroxylamine / Q000737:chemistry*; D057230:Limit of Detection; D008163:Luminescent Measurements; D053768:Metal Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000097:blood / Q000175:diagnosis; D020574:Proto-Oncogene Proteins c-sis / Q000032:analysis* / Q000276:immunology; D011817:Rabbits; D012680:Sensitivity and Specificity
chemical_list: D052157:Aptamers, Nucleotide; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D019811:Hydroxylamine; D006046:Gold