title: Sperm DNA damage has a negative association with live-birth rates after IVF.
abstract: Sperm DNA damage has a negative impact on pregnancy rates following assisted reproduction treatment (ART). The aim of the present study was to examine the relationship between sperm DNA fragmentation and live-birth rates after IVF and intracytoplasmic sperm injection (ICSI). The alkaline Comet assay was employed to measure sperm DNA fragmentation in native semen and in spermatozoa following density-gradient centrifugation in semen samples from 203 couples undergoing IVF and 136 couples undergoing ICSI. Men were divided into groups according to sperm DNA damage. Following IVF, couples with <25% sperm DNA fragmentation had a live-birth rate of 33%; in contrast, couples with >50% sperm DNA fragmentation had a much lower live-birth rate of 13%. Following ICSI, no significant differences in sperm DNA damage were found between any groups of patients. Sperm DNA damage was also associated with low live-birth rates following IVF in both men and couples with idiopathic infertility: 39% of couples and 41% of men with idiopathic infertility have high sperm DNA damage. Sperm DNA damage assessed by the Comet assay has a close inverse relationship with live-birth rates after IVF. Sperm DNA damage has a negative impact on assisted reproduction treatment outcome, in particular, on pregnancy rates. The aim of the present study was to examine the relationship between sperm DNA fragmentation and live-birth rates after IVF and intracytoplasmic sperm injection (ICSI). The alkaline Comet assay was employed to measure sperm DNA fragmentation in native semen and in spermatozoa following density-gradient centrifugation in semen samples from 203 couples undergoing IVF and 136 couples undergoing ICSI. Men were divided into groups according to sperm DNA damage and treatment outcome. Following IVF, couples with <25% sperm DNA fragmentation had a live birth rate of 33%. In contrast, couples with >50% sperm DNA fragmentation had a much lower live-birth rate of 13% following IVF. Following ICSI, there were no significant differences in levels of sperm DNA damage between any groups of patients. Sperm DNA damage was also associated with the very low live-birth rates following IVF in both men and couples with idiopathic infertility: 39% of couples and 41% of men have high level of sperm DNA damage. Sperm DNA damage assessed by the Comet assay has a close inverse relationship with live-birth rates after IVF.
authors.affiliation: Centre for Public Health, Institute of Clinical Sciences, Queen's University Belfast, Grosvenor Road, Belfast BT12 6BJ, Northern Ireland, UK.
authors.forename: L
authors.initials: L
authors.lastname: Simon
authors.forename: I
authors.initials: I
authors.lastname: Proutski
authors.forename: M
authors.initials: M
authors.lastname: Stevenson
authors.forename: D
authors.initials: D
authors.lastname: Jennings
authors.forename: J
authors.initials: J
authors.lastname: McManus
authors.forename: D
authors.initials: D
authors.lastname: Lutton
authors.forename: S E M
authors.initials: SE
authors.lastname: Lewis
mesh_terms: D000328:Adult; D020552:Comet Assay; D053938:DNA Fragmentation; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D050498:Live Birth; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate; D015203:Reproducibility of Results; D055101:Semen Analysis; D013094:Spermatozoa / Q000502:physiology*