title: Design, synthesis, and photochemistry of modular caging groups for photoreleasable nucleotides.
abstract: A modular approach to preparing caged nucleotides having additional properties has been achieved. The modular caging agent includes three components: an amine reactive NHS ester moiety, a photoactive Bhc group, and tosylhydrazone as a precursor of the diazomethyl group. Various amines including biotin and an Arg-Gly-Asp (RGD) peptide were introduced into the key intermediate via amide linkage. The Bio-Bhc-diazo thus synthesized enables the preparation of a photoreleasable siRNA with additional properties.
authors.affiliation: Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, 274-8510, Japan. furuta@biomol.sci.toho-u.ac.jp
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Furuta
authors.forename: Kaori
authors.initials: K
authors.lastname: Manabe
authors.forename: Aoi
authors.initials: A
authors.lastname: Teraoka
authors.forename: Kanako
authors.initials: K
authors.lastname: Murakoshi
authors.forename: Ai
authors.initials: A
authors.lastname: Ohtsubo
authors.forename: Akinobu
authors.initials: A
authors.lastname: Suzuki
mesh_terms: D006842:Hydrocarbons, Brominated / Q000737:chemistry; D008958:Models, Molecular; D009711:Nucleotides / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D055668:Photochemical Processes; D034741:RNA, Small Interfering / Q000737:chemistry; D012118:Resorcinols / Q000737:chemistry
chemical_list: D006842:Hydrocarbons, Brominated; D009711:Nucleotides; D009842:Oligopeptides; D034741:RNA, Small Interfering; D012118:Resorcinols; C047981:arginyl-glycyl-aspartic acid