title: Isolation and molecular characterization of Brucella melitensis from seropositive goats in Peninsula Malaysia.
abstract: A study was carried out to isolate Brucella melitensis using established   bacteriological and PCR techniques in Brucella seropositive goats in farms in Selangor, Negeri   Sembilan, Melaka and Pulau Pinang. Brucella melitensis was isolated from 7 of 134 reactors   with the highest isolation from the vaginal swabs (57.14%) followed by the spleen (28.57%),   uterine fluid (14.29%). No Brucella was isolated from the lymph nodes. PCR confirmed all the   seven isolates as B. melitensis and isolates were phylogenetically related to other isolates   from India, Iran, and Israel but most closely related to isolates from Singapore.
authors.affiliation: Dept of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: P H
authors.initials: PH
authors.lastname: Bamaiyi
authors.forename: L
authors.initials: L
authors.lastname: Hassan
authors.forename: S
authors.initials: S
authors.lastname: Khairani-Bejo
authors.forename: M
authors.initials: M
authors.lastname: Zainal Abidin
authors.forename: M
authors.initials: M
authors.lastname: Ramlan
authors.forename: N
authors.initials: N
authors.lastname: Krishnan
authors.forename: A
authors.initials: A
authors.lastname: Adzhar
authors.forename: N
authors.initials: N
authors.lastname: Abdullah
authors.forename: H M
authors.initials: HM
authors.lastname: Hamidah N
authors.forename: M M
authors.initials: MM
authors.lastname: Norsuhanna
authors.forename: S N
authors.initials: SN
authors.lastname: Hashim
mesh_terms: D000818:Animals; D017347:Brucella melitensis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D002006:Brucellosis / Q000382:microbiology / Q000662:veterinary*; D016000:Cluster Analysis; D005260:Female; D015511:Goat Diseases / Q000382:microbiology*; D006041:Goats; D008296:Malaysia; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013154:Spleen / Q000382:microbiology; D014599:Uterus / Q000382:microbiology; D014621:Vagina / Q000382:microbiology