title: Wide and scalable field-of-view Talbot-grid-based fluorescence microscopy.
abstract: Here we report a low-cost and simple wide field-of-view (FOV) on-chip fluorescence-imaging platform, termed fluorescence Talbot microscopy (FTM), which utilizes the Talbot self-imaging effect to enable efficient fluorescence imaging over a large and directly scalable FOV. The FTM prototype has a resolution of 1.2 μm and an FOV of 3.9 mm × 3.5 mm. We demonstrate the imaging capability of FTM on fluorescently labeled breast cancer cells (SK-BR-3) and human embryonic kidney 293 (HEK) cells expressing green fluorescent protein.
authors.affiliation: Department of Electrical Engineering, California Institute of Technology, Pasadena, California 91125, USA. spang@caltech.edu
authors.forename: Shuo
authors.initials: S
authors.lastname: Pang
authors.forename: Chao
authors.initials: C
authors.lastname: Han
authors.forename: Mihoko
authors.initials: M
authors.lastname: Kato
authors.forename: Paul W
authors.initials: PW
authors.lastname: Sternberg
authors.forename: Changhuei
authors.initials: C
authors.lastname: Yang
mesh_terms: D057809:HEK293 Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors