title: [HBV genotypes distribution and YMDD spontaneous mutation in Qingdao population].
abstract: OBJECTIVE
  To investigate the distribution of hepatitis B virus (HBV) genotypes in Qingdao, and the relationship of HBV genotypes with the serum HBV-DNA levels and HBV YMDD spontaneous mutation of patients, then to discuss the clinical significance.
  
  
  METHODS
  Hepatitis B virus genotypes and YMDD spontaneous mutation of 144 patients were detected by real time PCR (Taqman probe), then the results were analyzed by statistical method.
  
  
  RESULTS
  Of the 144 patients, 130 (90.3%) were genotype C, 12 (8.3%) were genotype B, and 2 (1.4%) were neither genotype B nor genotype C; 33 (22.9%) were detected to have YMDD mutation, and 25 (75.5%) were YVDD positive, 3 (9.1%) were YIDD positive, 5 (15.2%) were YVDD and YIDD positive. There were no significant differences between clinical diagnosis, serum HBV-DNA levels, YMDD spontaneous mutation and HBV genotypes (P > 0.05).
  
  
  CONCLUSIONS
  Genotype C is the dominant position for HBV genotype in Qingdao. Untreated patients with chronic hepatitis B have YMDD spontaneous mutation. HBV genotypes have no association with YMDD spontaneous mutation and the development of diseases.
authors.affiliation: Qingdao Municipal Hospital, Qingdao 266071, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Wang
authors.forename: Xin-Juan
authors.initials: XJ
authors.lastname: Yu
authors.forename: Quan-Jiang
authors.initials: QJ
authors.lastname: Dong
authors.forename: Hua-Bo
authors.initials: HB
authors.lastname: Chen
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Zhou
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002681:China; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005838:Genotype; D006515:Hepatitis B virus / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D055815:Young Adult
chemical_list: D014764:Viral Proteins; D004259:DNA-Directed DNA Polymerase