title: A comparative uptake study of multiplexed PET tracers in mice with turpentine-induced inflammation.
abstract: The potential value of multiplexed positron emission tomography (PET) tracers in mice with turpentine-induced inflammation was evaluated and compared with 2-[¹⁸F]fluoro-2-deoxy-D-glucose ([¹⁸F]FDG) for glucose metabolism imaging. These PET tracers included [¹⁸F]fluoromethylcholine ([¹⁸F]FCH) for choline metabolism imaging, (S-[¹¹C]methyl)-D-cysteine ([¹¹C]DMCYS) for amino acid metabolism imaging, [¹¹C]bis(zinc(II)-dipicolylamine) ([¹¹C]DPA-Zn²⁺) for apoptosis imaging, 2-(4-N-[¹¹C]-methylaminophenyl)-6-hydroxybenzothiazole ([¹¹C]PIB) for β amyloid binding imaging, and [¹⁸F]fluoride (¹⁸F⁻) for bone metabolism imaging. In mice with turpentine-induced inflammation mice, the biodistribution of all the tracers mentioned above at 5, 15, 30, 45, and 60 min postinjection was determined. Also, the time-course curves of the tracer uptake ratios for inflammatory thigh muscle (IM) to normal uninflammatory thigh muscle (NM), IM to blood (BL), IM to brain (BR), and IM to liver (LI) were acquired, respectively. Moreover, PET imaging with the tracers within 60 min postinjection on a clinical PET/CT scanner was also conducted. [¹⁸F]FDG and ¹⁸F⁻ showed relatively higher uptake ratios for IM to NM, IM to BL, IM to BR, and IM to LI than [¹⁸F]FCH, [¹¹C]DPA-Zn²⁺, [¹¹C]DMCYS and [¹¹C]PIB, which were highly consistent with the results delineated in PET images. The results demonstrate that ¹⁸F⁻ seems to be a potential PET tracer for inflammation imaging. [¹⁸F]FCH and [¹¹C]DMCYS, with lower accumulation in inflammatory tissue than [¹⁸F]FDG, are not good PET tracers for inflammation imaging. As a promising inflammatory tracer, the chemical structure of [¹¹C]DPA-Zn²⁺ needs to be further optimized.
authors.affiliation: Department of Nuclear Medicine, The First Afﬁliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China. mandyhtt@163.com
authors.forename: Tingting
authors.initials: T
authors.lastname: Huang
authors.forename: Hongliang
authors.initials: H
authors.lastname: Wang
authors.forename: Ganghua
authors.initials: G
authors.lastname: Tang
authors.forename: Xiang
authors.initials: X
authors.lastname: Liang
authors.forename: Dahong
authors.initials: D
authors.lastname: Nie
authors.forename: Chang
authors.initials: C
authors.lastname: Yi
authors.forename: Kening
authors.initials: K
authors.lastname: Wu
mesh_terms: D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D003952:Diagnostic Imaging; D005462:Fluorine Radioisotopes; D019788:Fluorodeoxyglucose F18; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000175:diagnosis; D051379:Mice; D049268:Positron-Emission Tomography / Q000379:methods*; D011849:Radioactive Tracers; D013844:Thiazoles; D014018:Tissue Distribution; D014425:Turpentine / Q000633:toxicity
chemical_list: D005462:Fluorine Radioisotopes; D011849:Radioactive Tracers; D013844:Thiazoles; D019788:Fluorodeoxyglucose F18; C077548:6-hydroxybenzothiazole; D014425:Turpentine