title: Two retroviruses packaged in one cell line can combined inhibit the replication of HIV-1 in TZM-bl cells.
abstract: The cellular protein tetherin tethers the HIV-1 viral particles on the cellular membrane to inhibit the replication of HIV-1. However, the HIV-1 accessory protein Vpu counteracts the antiviral function of tetherin. In this study, two retroviral vector plasmids were constructed. One inhibited the vpu gene expression; the other one over-expressed the tetherin. Both retroviral vector plasmids could be packaged in the packaging cell line PT67 to obtain the corresponding retroviruses. The retroviral vector plasmids' functions of tetherin over-expression or vpu-RNAi were detected at the cell level. Retroviral vector plasmids were transfected to PT67 cells at different ratios from 0T3V to 3T0V, and then mixed retroviruses were harvested. The antiviral functions of mixed retroviruses were detected in HIV-1 infected TZM-bl cells. The results showed that packaged mixed retroviruses could repress the replication of HIV-1 in TZM-bl cells.
authors.affiliation: School of Medicine, Nankai University, Tianjin 300071, China.
authors.forename: Zhipin
authors.initials: Z
authors.lastname: Liang
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Guo
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Kong
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
mesh_terms: D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics; D002460:Cell Line; D004847:Epithelial Cells / Q000821:virology; D058851:GPI-Linked Proteins / Q000096:biosynthesis / Q000235:genetics; D015870:Gene Expression; D015497:HIV-1 / Q000502:physiology*; D054298:Human Immunodeficiency Virus Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D006801:Humans; D010957:Plasmids; D054334:Viral Regulatory and Accessory Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D019065:Virus Assembly; D014779:Virus Replication; D000074010:Bone Marrow Stromal Antigen 2; D000086082:Viroporin Proteins
chemical_list: D015703:Antigens, CD; D058851:GPI-Linked Proteins; D054298:Human Immunodeficiency Virus Proteins; D054334:Viral Regulatory and Accessory Proteins; C094572:BST2 protein, human; D000074010:Bone Marrow Stromal Antigen 2; C514859:vpu protein, Human immunodeficiency virus 1; D000086082:Viroporin Proteins