title: Development of a new rabbit monoclonal antibody and its based competitive indirect enzyme-linked immunosorbent assay for rapid detection of sulfonamides.
abstract: BACKGROUND
  Monoclonal antibodies generally obtained through the classic mouse hybridoma system were requisite for the establishment of various immunoassays. In this study, a new rabbit monoclonal antibody (RabMAb) against sulfonamides (SAs) was first produced via hybridoma technique in rabbit. The related enzyme-linked immunosorbent assay (ELISA) was then developed and applied to real sample analysis.
  
  
  RESULTS
  A sensitive competitive indirect ELISA method based on a novel RabMAb for rapid detection of sulfonamides was first established. The obtained half-maximum inhibition concentration (IC(50)) values for four SAs were all below 10 ng mL(-1) , with 0.68 ng mL(-1) sulfathiazole (STZ), 1.11 ng mL(-1) sulfadiazine (SD), 1.15 ng mL(-1) sulfapyridine (SP) and 5.27 ng mL(-1) sulfamethoxazole (SMX). Desirable recoveries when detecting different spiked swine urine and milk samples were achieved ranging from 92.6% to 104.3% and from 61.1% to 81.6%, respectively.
  
  
  CONCLUSIONS
  The proposed immunoassay with the newly developed RabMAb is capable of detection of four SAs (STZ, SD, SP and SMX) with proven satisfactory performance and is applicable for routine large-scale analysis in practical uses.
authors.affiliation: School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Han
authors.forename: Lei
authors.initials: L
authors.lastname: Lu
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Geng
authors.initials: G
authors.lastname: Ni
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Zhao
authors.forename: Aibo
authors.initials: A
authors.lastname: Wu
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zheng
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D008892:Milk / Q000737:chemistry; D011817:Rabbits; D000077589:Sulfathiazole; D013432:Sulfathiazoles / Q000276:immunology; D013449:Sulfonamides / Q000032:analysis* / Q000276:immunology* / Q000652:urine; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal; D013432:Sulfathiazoles; D013449:Sulfonamides; D000077589:Sulfathiazole