title: In vitro activities of 11 antimicrobial agents against macrolide-resistant Mycoplasma pneumoniae isolates from pediatric patients: results from a multicenter surveillance study.
abstract: Macrolide-resistant Mycoplasma pneumoniae is emerging in several countries, and it is mainly observed in children. To our knowledge, we conducted the first multicenter prospective epidemiological study of macrolide-resistant M. pneumoniae in order to investigate regional differences in the susceptibility of macrolide-resistant M. pneumoniae to antibacterial agents. The in vitro activities of 11 antimicrobial agents against macrolide-resistant M. pneumoniae isolates from 5 different areas of Japan were investigated. Among 190 M. pneumoniae isolates from pediatric patients, 124 (65.2%) isolates showed macrolide resistance and possessed an A2063G transition in domain V of the 23S rRNA. These isolates showed high resistance to erythromycin, clarithromycin, and azithromycin with minimum inhibitory concentrations (MICs) ≥ 16 μg/ml. Conversely, quinolones such as garenoxacin, moxifloxacin, tosufloxacin, and levofloxacin exhibited potent antimycoplasmal activity. No regional differences were observed with respect to the MICs among the 5 areas in Japan.
authors.affiliation: Department of Pediatrics, Kawasaki Medical School, Okayama, Japan.
authors.forename: Hiroto
authors.initials: H
authors.lastname: Akaike
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Miyashita
authors.forename: Mika
authors.initials: M
authors.lastname: Kubo
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kawai
authors.forename: Takaaki
authors.initials: T
authors.lastname: Tanaka
authors.forename: Satoko
authors.initials: S
authors.lastname: Ogita
authors.forename: Kozo
authors.initials: K
authors.lastname: Kawasaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakano
authors.forename: Kihei
authors.initials: K
authors.lastname: Terada
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Ouchi
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D002648:Child; D002675:Child, Preschool; D024881:Drug Resistance, Bacterial; D005260:Female; D006801:Humans; D007564:Japan; D018942:Macrolides / Q000494:pharmacology*; D008297:Male; D008826:Microbial Sensitivity Tests; D009175:Mycoplasma Infections / Q000382:microbiology; D009177:Mycoplasma pneumoniae / Q000187:drug effects* / Q000302:isolation & purification; D017354:Point Mutation; D012329:RNA, Bacterial / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics
chemical_list: D000890:Anti-Infective Agents; D018942:Macrolides; D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S