title: Biological characteristics and propagation of human rhinovirus-C in differentiated sinus epithelial cells.
abstract: Information about the basic biological properties of human rhinovirus-C (HRV-C) viruses is lacking due to difficulties with culturing these viruses. Our objective was to develop a cell culture system to grow HRV-C. Epithelial cells from human sinuses (HSEC) were differentiated at air-liquid interface (ALI). Differentiated cultures supported 1-2 logs growth of HRV-C15 as detected by quantitative RT-PCR. Two distinguishing features of HRVs are acid lability and optimal growth at 33-34 °C. We used this system to show that HRV-C15 is neutralized by low pH (4.5). In contrast to most HRV types, replication of HRV-C15 and HRV-C41 was similar at 34 and 37°C. The HSEC ALI provides a useful tool for quantitative studies of HRV-C replication. The ability of HRV-C to grow equally well at 34°C and 37°C may contribute to the propensity for HRV-C to cause lower airway illnesses in infants and children with asthma.
authors.affiliation: Department of Pediatrics, School of Medicine and Public Health, University of Wisconsin, Madison, WI, United States.
authors.forename: Shamaila
authors.initials: S
authors.lastname: Ashraf
authors.forename: Rebecca
authors.initials: R
authors.lastname: Brockman-Schneider
authors.forename: Yury A
authors.initials: YA
authors.lastname: Bochkov
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Pasic
authors.forename: James E
authors.initials: JE
authors.lastname: Gern
mesh_terms: D002460:Cell Line; D004847:Epithelial Cells / Q000821:virology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010256:Paranasal Sinuses / Q000166:cytology / Q000821:virology*; D012229:Rhinovirus / Q000145:classification / Q000254:growth & development* / Q000378:metabolism; D013696:Temperature; D014776:Virus Cultivation; D014779:Virus Replication