title: Recognition and enrichment specificity of Fe₃O₄ magnetic nanoparticles surface modified by chitosan and Staphylococcus aureus enterotoxins A antiserum.
abstract: Fe(3)O(4) modified by chitosan (CTS) and Staphylococcus aureus enterotoxin A (SEA) antiserum immunomagnetic beads (Fe(3)O(4)-CTS-SEA-IB) that targeted enrich SEA were prepared in aqueous solution and using CTS and SEA antiserum as surface modification stabilizer. Recognition experiments of the prepared Fe(3)O(4)-CTS-SEA-IB on SEA were conducted to determine adsorption capacity and recognition specificity. The results showed that the SEA antiserum were prepared and successfully conjugated onto Fe(3)O(4)-modificated CTS magnetic beads. The Fe(3)O(4)-CTS-SEA immunomagnetic beads displayed a high adsorption capacity and recognition specificity for SEA, and the adsorption quantity could reach 6.48 × 10(-3) μmol/g. The specificity evaluation results showed that the Fe(3)O(4)-CTS-SEA immunomagnetic beads had high enrichment and affinity property for SEA compared to SEB (Staphylococcus aureus enterotoxin B) and SPA (Staphylococcus aureus protein A).
authors.affiliation: College of Tea & Food Science and Technology, Anhui Agricultural University, Key Laboratory of Analysis and Detection Technology for Food Safety, Hefei, Anhui 230036, China.
authors.forename: Xiuheng
authors.initials: X
authors.lastname: Xue
authors.forename: Juhua
authors.initials: J
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Mei
authors.forename: Zhigeng
authors.initials: Z
authors.lastname: Wang
authors.forename: Kezong
authors.initials: K
authors.lastname: Qi
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
mesh_terms: D000327:Adsorption; D000818:Animals; D000941:Antigens / Q000276:immunology; D048271:Chitosan / Q000737:chemistry*; D004768:Enterotoxins / Q000737:chemistry / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007106:Immune Sera / Q000276:immunology*; D060328:Magnetic Phenomena; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008863:Microspheres; D011817:Rabbits; D017550:Spectroscopy, Fourier Transform Infrared; D013205:Staphylococcal Protein A / Q000276:immunology; D013499:Surface Properties; D013696:Temperature
chemical_list: D000941:Antigens; D004768:Enterotoxins; D007106:Immune Sera; D058185:Magnetite Nanoparticles; D013205:Staphylococcal Protein A; C026066:enterotoxin A, Staphylococcal; C031818:enterotoxin B, staphylococcal; D048271:Chitosan