title: The Arg719 residue at the C-terminal end of the stem region in hepatitis C virus JFH-1 E2 glycoprotein promotes viral infection.
abstract: Hepatitis C virus (HCV) envelope glycoprotein E2 is involved in virus assembly and initial entry into host cells. The tertiary organization of the E2 ectodomain is mainly composed of domains I-III, followed by the stem (ST) region and transmembrane (TM) domain. The ST region is critical for reorganizing the envelope glycoproteins during the membrane fusion process. While this region is relatively flexible, the physicochemical properties of its amino acid residues are conserved. Whether and how this physicochemical conservation is required for HCV infection is still unclear. The last residue of the E2 ST region evolved to be either an arginine or lysine among different HCV strains, suggesting that the residues confer different functions during HCV infection. To address this possibility, we constructed an R719K point mutant in the JFH-1 strain (genotype 2a) in the context of the cell-culture derived HCV (HCVcc) system. Compared with wild-type (wt) HCV, the R719K mutant exhibited decreased growth, and its extracellular and intracellular infectivity were also significantly decreased at 48 and 72 h post-electroporation. Correspondingly, less RNA and HCV core protein was observed in the supernatant for the R719K mutant, as well as less efficient RNA replication and protein expression. These findings indicate that the 719th residue of arginine on E2 is critical to promote HCV replication and infection. The data provide new clues for the biochemical function of E2, which is required for efficient HCV assembly and infection.
authors.affiliation: Department of Microbiology, Shanghai Key Laboratory of Medical Biodefense, Second Military Medical University, Shanghai 200433, China.
authors.forename: Zhao-ling
authors.initials: ZL
authors.lastname: Qin
authors.forename: He-peng
authors.initials: HP
authors.lastname: Ju
authors.forename: Wen-bo
authors.initials: WB
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Ren
authors.forename: Mo
authors.initials: M
authors.lastname: Guan
authors.forename: Ping
authors.initials: P
authors.lastname: Zhao
authors.forename: Zhong-tian
authors.initials: ZT
authors.lastname: Qi
mesh_terms: D019943:Amino Acid Substitution; D001120:Arginine / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D016174:Hepacivirus / Q000472:pathogenicity / Q000502:physiology*; D022781:Hepatocytes / Q000821:virology; D006801:Humans; D016297:Mutagenesis, Site-Directed; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D050505:Mutant Proteins; D012367:RNA, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D037521:Virulence Factors; C091015:glycoprotein E2, Hepatitis C virus; D001120:Arginine