title: Immunological determinants in a murine model of toluene diisocyanate-induced asthma.
abstract: OBJECTIVE
  Diisocyanates (DIC) are highly reactive, low-molecular-weight chemicals which are the leading cause of occupational asthma (OA). The aim of the study was to analyze certain aspects of the pathogenesis of allergic inflammation in the airways induced by toluene diisocyanate (TDI) in an experimental model in mice.
  
  
  METHODS
  The experiment was carried out on 50 female BALB/cJ/Han/IMP mice, which were exposed by inhalation (intranasal and in the inhalation chamber) to toluene diisocyanate (2,4-TDI). After the experiment, the bronchoalveolar lavage fluid (BALF) was collected from the animals, and the composition of the induced inflammatory cells, and the concentrations of certain cytokines (IL-4, IL-5, TNF-α) were evaluated.
  
  
  RESULTS
  The total number of cells in BALF of the examined group of mice was significantly higher compared to the control mice. There was also a significant increase in neutrophils and eosinophils in the study group compared to the controls. The number of lymphocytes and macrophages did not differ significantly between the two groups. A statistically significant increase in the level of TNF-α was shown to occur in the group exposed to toluene diisocyanate in comparison to the control group. The concentration of IL-4 increased in the study group, compared to the control one, but the differences did not reach the level of significance, p > 0.05. Such difference was not observed for IL-5.
  
  
  CONCLUSIONS
  We developed a murine model of TDI-induced asthma which caused the influx of inflammatory cells like eosinophils and neutrophils in the bronchoalveolar lavage fluid (BALF) in the TDI-treated mice. The increase of the concentration of some proinflammatory cytokines (TNF-α, IL-4) in BALF from the exposed mice was also observed.
authors.affiliation: Centre of Occupational Allergy and Environmental Health, Nofer Institute of Occupational Medicine, Łódź, Poland. mika@imp.lodz.pl
authors.forename: Dominika
authors.initials: D
authors.lastname: Świerczyńska-Machura
authors.forename: Jolanta
authors.initials: J
authors.lastname: Walusiak-Skorupa
authors.forename: Ewa
authors.initials: E
authors.lastname: Nowakowska-Świrta
authors.forename: Joanna
authors.initials: J
authors.lastname: Piasecka-Zelga
authors.forename: Radosław
authors.initials: R
authors.lastname: Świercz
authors.forename: Cezary
authors.initials: C
authors.lastname: Pałczyński
mesh_terms: D000818:Animals; D001249:Asthma / Q000139:chemically induced* / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology*; D002452:Cell Count; D016207:Cytokines / Q000032:analysis; D004195:Disease Models, Animal; D005260:Female; D019570:Inhalation Exposure / Q000009:adverse effects*; D007962:Leukocytes; D008264:Macrophages; D051379:Mice; D014051:Toluene 2,4-Diisocyanate / Q000009:adverse effects*
chemical_list: D016207:Cytokines; D014051:Toluene 2,4-Diisocyanate