title: Nanoroughened surfaces for efficient capture of circulating tumor cells without using capture antibodies.
abstract: Circulating tumor cells (CTCs) detached from both primary and metastatic lesions represent a potential alternative to invasive biopsies as a source of tumor tissue for the detection, characterization and monitoring of cancers. Here we report a simple yet effective strategy for capturing CTCs without using capture antibodies. Our method uniquely utilized the differential adhesion preference of cancer cells to nanorough surfaces when compared to normal blood cells and thus did not depend on their physical size or surface protein expression, a significant advantage as compared to other existing CTC capture techniques.
authors.affiliation: Integrated Biosystems and Biomechanics Laboratory, University of Michigan, Ann Arbor, Michigan 48109, USA.
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Chen
authors.forename: Shinuo
authors.initials: S
authors.lastname: Weng
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Steven
authors.initials: S
authors.lastname: Allen
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Liwei
authors.initials: L
authors.lastname: Bao
authors.forename: Raymond H W
authors.initials: RH
authors.lastname: Lam
authors.forename: Jill A
authors.initials: JA
authors.lastname: Macoska
authors.forename: Sofia D
authors.initials: SD
authors.lastname: Merajver
authors.forename: Jianping
authors.initials: J
authors.lastname: Fu
mesh_terms: D000906:Antibodies / Q000276:immunology*; D002469:Cell Separation / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D061986:MCF-7 Cells; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D009360:Neoplastic Cells, Circulating / Q000276:immunology* / Q000473:pathology*; D010316:Particle Size; D013499:Surface Properties
chemical_list: D000906:Antibodies