title: Wound dressings based on silver sulfadiazine solid lipid nanoparticles for tissue repairing.
abstract: The management of difficult to heal wounds can considerably reduce the time required for tissue repairing and promote the healing process, minimizing the risk of infection. Silver compounds, especially silver sulfadiazine (AgSD), are often used to prevent or to treat wound colonization, also in presence of antibiotic-resistant bacteria. However, AgSD has been shown to be cytotoxic in vitro toward fibroblasts and keratinocytes and consequently to retard wound healing in vivo. Recently, platelet lysate (PL) has been proposed in clinical practice for the healing of persistent lesions. The aim of the present work was the development of wound dressings based on AgSD loaded in solid lipid nanoparticles (SLNs), to be used in association with PL for the treatment for skin lesions. SLN were based on chondroitin sulfate and sodium hyaluronate, bioactive polymers characterized by well-known tissue repairing properties. The encapsulation of AgSD in SLN aimed at preventing the cytotoxic effect of the drug on normal human dermal fibroblasts (NHDFs) and at enabling the association of the drug with PL. SLN were loaded in wound dressings based on hydroxypropylmethyl cellulose (HPMC) or chitosan glutamate (CS glu). These polymers were chosen to obtain a sponge matrix with suitable elasticity and softness and, moreover, with good bioadhesive behavior on skin lesions. Dressings based on chitosan glutamate showed antimicrobial activity with and without PL. Even though further in vivo evaluation could be envisaged, chitosan based dressings demonstrated to be a suitable prototype for the treatment for skin lesions.
authors.affiliation: Department of Drug Sciences, University of Pavia, Pavia, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Sandri
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Bonferoni
authors.forename: Francesca
authors.initials: F
authors.lastname: D'Autilia
authors.forename: Silvia
authors.initials: S
authors.lastname: Rossi
authors.forename: Franca
authors.initials: F
authors.lastname: Ferrari
authors.forename: Pietro
authors.initials: P
authors.lastname: Grisoli
authors.forename: Milena
authors.initials: M
authors.lastname: Sorrenti
authors.forename: Laura
authors.initials: L
authors.lastname: Catenacci
authors.forename: Claudia
authors.initials: C
authors.lastname: Del Fante
authors.forename: Cesare
authors.initials: C
authors.lastname: Perotti
authors.forename: Carla
authors.initials: C
authors.lastname: Caramella
mesh_terms: D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D006801:Humans; D008055:Lipids; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D009779:Occlusive Dressings; D010316:Particle Size; D012837:Silver Sulfadiazine / Q000008:administration & dosage* / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D000891:Anti-Infective Agents, Local; D008055:Lipids; D012837:Silver Sulfadiazine