title: Expression, crystallization and preliminary X-ray crystallographic analysis of cystathionine γ-synthase (XometB) from Xanthomonas oryzae pv. oryzae.
abstract: Cystathionine γ-synthase (CGS) catalyzes the first step in the transsulfuration pathway leading to the formation of cystathionine from O-succinylhomoserine and L-cysteine through a γ-replacement reaction. As an antibacterial drug target against Xanthomonas oryzae pv. oryzae (Xoo), CGS from Xoo (XometB) was cloned, expressed, purified and crystallized. The XometB crystal diffracted to 2.4 Å resolution and belonged to the tetragonal space group I4(1), with unit-cell parameters a=b=165.4, c=241.7 Å. There were four protomers in the asymmetric unit, with a corresponding solvent content of 73.9%.
authors.affiliation: Department of Advanced Technology Fusion, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Ho-Phuong-Thuy
authors.initials: HP
authors.lastname: Ngo
authors.forename: Jin-Kwang
authors.initials: JK
authors.lastname: Kim
authors.forename: Seung-Hwan
authors.initials: SH
authors.lastname: Kim
authors.forename: Tan-Viet
authors.initials: TV
authors.lastname: Pham
authors.forename: Thi-Huyen
authors.initials: TH
authors.lastname: Tran
authors.forename: Dinh-Duc
authors.initials: DD
authors.lastname: Nguyen
authors.forename: Jeong-Gu
authors.initials: JG
authors.lastname: Kim
authors.forename: Sumi
authors.initials: S
authors.lastname: Chung
authors.forename: Yeh-Jin
authors.initials: YJ
authors.lastname: Ahn
authors.forename: Lin-Woo
authors.initials: LW
authors.lastname: Kang
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D019757:Carbon-Oxygen Lyases / Q000737:chemistry* / Q000378:metabolism; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D014974:Xanthomonas / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C022696:O-succinylhomoserine (thiol)-lyase; D019757:Carbon-Oxygen Lyases