title: Carbon catabolite repression in Thermoanaerobacterium saccharolyticum.
abstract: BACKGROUND
  The thermophilic anaerobe Thermoanaerobacterium saccharolyticum is capable of directly fermenting xylan and the biomass-derived sugars glucose, cellobiose, xylose, mannose, galactose and arabinose. It has been metabolically engineered and developed as a biocatalyst for the production of ethanol.
  
  
  RESULTS
  We report the initial characterization of the carbon catabolite repression system in this organism. We find that sugar metabolism in T. saccharolyticum is regulated by histidine-containing protein HPr. We describe a mutation in HPr, His15Asp, that leads to derepression of less-favored carbon source utilization.
  
  
  CONCLUSIONS
  Co-utilization of sugars can be achieved by mutation of HPr in T. saccharolyticum. Further manipulation of CCR in this organism will be instrumental in achieving complete and rapid conversion of all available sugars to ethanol.
authors.affiliation: Mascoma Corporation, 67 Etna Road, Suite 300, New Hampshire, 03766, Lebanon. cherring@mascoma.com.
authors.forename: Vasiliki
authors.initials: V
authors.lastname: Tsakraklides
authors.forename: A Joe
authors.initials: AJ
authors.lastname: Shaw
authors.forename: Bethany B
authors.initials: BB
authors.lastname: Miller
authors.forename: David A
authors.initials: DA
authors.lastname: Hogsett
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Herring