title: A DSC investigation on the influence of gemini surfactant stereochemistry on the organization of lipoplexes and on their interaction with model membranes.
abstract: Previous investigations showed that the extent of DNA condensation and the efficiency in the transfection of liposomes formulated with 1,2-dimyristoyl-sn-glycero-phosphocholine and cationic stereomeric gemini surfactants depend heavily on the stereochemistry of the gemini. The influence of the stereochemistry on the interaction of lipoplexes with zwitterionic and anionic cell membrane models was investigated by differential scanning calorimetry to rationalize their different biological behavior. Further, the thermotropic behavior of the corresponding liposomes and of the spontaneous self-assemblies of gemini surfactants in the presence and in the absence of DNA was evaluated to correlate the physicochemical properties of lipoplexes and the stereochemistry of the cationic component. The obtained results show that the stereochemistry of the gemini surfactant controls lipoplexes organization and their mode and kinetic of interaction with different cell membrane models.
authors.affiliation: Dipartimento di Chimica Università degli Studi di Roma Sapienza, P.le A. Moro 5, 00185 Roma, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Aleandri
authors.forename: M G
authors.initials: MG
authors.lastname: Bonicelli
authors.forename: L
authors.initials: L
authors.lastname: Giansanti
authors.forename: C
authors.initials: C
authors.lastname: Giuliani
authors.forename: M
authors.initials: M
authors.lastname: Ierino
authors.forename: G
authors.initials: G
authors.lastname: Mancini
authors.forename: A
authors.initials: A
authors.lastname: Martino
authors.forename: A
authors.initials: A
authors.lastname: Scipioni
mesh_terms: D002117:Calcitriol / Q000031:analogs & derivatives* / Q000737:chemistry; D002152:Calorimetry, Differential Scanning; D002412:Cations / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D008956:Models, Chemical; D013237:Stereoisomerism; D013501:Surface-Active Agents / Q000737:chemistry*; D044366:Transition Temperature
chemical_list: C411462:1,25-dihydroxy-21-(3-hydroxy-3-methylbutyl)vitamin D(3); D002412:Cations; D008081:Liposomes; D013501:Surface-Active Agents; D004247:DNA; D002117:Calcitriol; D004134:Dimyristoylphosphatidylcholine