title: A novel injectable temperature-sensitive zinc doped chitosan/β-glycerophosphate hydrogel for bone tissue engineering.
abstract: Hydrogels are hydrophilic polymers that have a wide range of biomedical applications including bone tissue engineering. In this study we report preparation and characterization of a thermosensitive hydrogel (Zn-CS/β-GP) containing zinc (Zn), chitosan (CS) and beta-glycerophosphate (β-GP) for bone tissue engineering. The prepared hydrogel exhibited a liquid state at room temperature and turned into a gel at body temperature. The hydrogel was characterized by SEM, EDX, XRD, FT-IR and swelling studies. The hydrogel enhanced antibacterial activity and promoted osteoblast differentiation. Thus, we suggest that the Zn-CS/β-GP hydrogel could have potential impact as an injectable in situ forming scaffold for bone tissue engineering applications.
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603 203, TamilNadu, India.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Niranjan
authors.forename: Chandru
authors.initials: C
authors.lastname: Koushik
authors.forename: Sekaran
authors.initials: S
authors.lastname: Saravanan
authors.forename: Ambigapathi
authors.initials: A
authors.lastname: Moorthi
authors.forename: Mariappanadar
authors.initials: M
authors.lastname: Vairamani
authors.forename: Nagarajan
authors.initials: N
authors.lastname: Selvamurugan
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001842:Bone and Bones / Q000187:drug effects*; D016923:Cell Death / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D048271:Chitosan / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D005994:Glycerophosphates / Q000494:pharmacology*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007267:Injections; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects; D013696:Temperature; D013997:Time Factors; D023822:Tissue Engineering; D014961:X-Ray Diffraction; D015032:Zinc / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D005994:Glycerophosphates; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D048271:Chitosan; D015032:Zinc; C031463:beta-glycerophosphoric acid