title: Incidence and antibiotic susceptibility of Mycoplasma hominis and Ureaplasma urealyticum isolated in Brescia, Italy, over 7 years.
abstract: The prevalence and antimicrobial susceptibility of Ureaplasma urealyticum and Mycoplasma hominis collected during 2004-2011 were determined. A total of 9956 individuals was analyzed. Identification was performed by use of the mycoplasma IST-2 kit. Antimicrobial susceptibility against doxycycline, josamycin, ofloxacin, erythromycin, tetracycline, ciprofloxacin, azithromycin, clarithromycin, and pristinamycin was also tested by use of this commercial kit. Our results show a prevalence of 1856 positive patients for genital mycoplasmas (18.6 %). Among positive cultures, 89 and 1.1 % of isolates were Ureaplasma urealyticum and Mycoplasma hominis, respectively. For 9.8 % of isolates both urogenital mycoplasmas were grown. Doxycycline was the most active tetracycline for mycoplasma infections, and this is still the drug of first choice. Among macrolides, josamycin and clarithromycin are the most active agents against ureaplasmas; josamycin is also active against mycoplasmas and is an alternative to tetracyclines and erythromycin for mixed infections, especially for pregnant women and neonates. Fluoroquinolones had low efficacy against urogenital mycoplasmas. For Ureaplasma urealyticum, cross-resistance was found between erythromycin and macrolides (except josamycin) (40-80 %) and between erythromycin and ciprofloxacin (79 %). Antibiotic resistance over the test period did not vary significantly. Because of geographical differences among antibiotic resistance, local in-vitro susceptibility testing is recommended to avoid failure of therapy.
authors.affiliation: Department of Experimental and Applied Medicine, Institute of Microbiology, University of Brescia, P. le Spedali Civili, 1, 25123, Brescia, Italy. defrance@med.unibs.it
authors.forename: Maria Antonia
authors.initials: MA
authors.lastname: De Francesco
authors.forename: Sonia
authors.initials: S
authors.lastname: Caracciolo
authors.forename: Carlo
authors.initials: C
authors.lastname: Bonfanti
authors.forename: Nino
authors.initials: N
authors.lastname: Manca
mesh_terms: D000293:Adolescent; D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016009:Chi-Square Distribution; D024881:Drug Resistance, Bacterial; D005260:Female; D006801:Humans; D015994:Incidence; D007558:Italy / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D009175:Mycoplasma Infections / Q000453:epidemiology / Q000382:microbiology*; D019535:Mycoplasma hominis / Q000187:drug effects / Q000302:isolation & purification*; D012189:Retrospective Studies; D016869:Ureaplasma Infections / Q000453:epidemiology / Q000382:microbiology*; D016990:Ureaplasma urealyticum / Q000187:drug effects / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents