title: An RNA-Seq transcriptome analysis of orthophosphate-deficient white lupin reveals novel insights into phosphorus acclimation in plants.
abstract: Phosphorus, in its orthophosphate form (P(i)), is one of the most limiting macronutrients in soils for plant growth and development. However, the whole-genome molecular mechanisms contributing to plant acclimation to P(i) deficiency remain largely unknown. White lupin (Lupinus albus) has evolved unique adaptations for growth in P(i)-deficient soils, including the development of cluster roots to increase root surface area. In this study, we utilized RNA-Seq technology to assess global gene expression in white lupin cluster roots, normal roots, and leaves in response to P(i) supply. We de novo assembled 277,224,180 Illumina reads from 12 complementary DNA libraries to build what is to our knowledge the first white lupin gene index (LAGI 1.0). This index contains 125,821 unique sequences with an average length of 1,155 bp. Of these sequences, 50,734 were transcriptionally active (reads per kilobase per million reads ≥ 3), representing approximately 7.8% of the white lupin genome, using the predicted genome size of Lupinus angustifolius as a reference. We identified a total of 2,128 sequences differentially expressed in response to P(i) deficiency with a 2-fold or greater change and P ≤ 0.05. Twelve sequences were consistently differentially expressed due to P(i) deficiency stress in three species, Arabidopsis (Arabidopsis thaliana), potato (Solanum tuberosum), and white lupin, making them ideal candidates to monitor the P(i) status of plants. Additionally, classic physiological experiments were coupled with RNA-Seq data to examine the role of cytokinin and gibberellic acid in P(i) deficiency-induced cluster root development. This global gene expression analysis provides new insights into the biochemical and molecular mechanisms involved in the acclimation to P(i) deficiency.
authors.affiliation: United States Department of Agriculture-Agricultural Research Service, Plant Science Research Unit, St. Paul, Minnesota 55108, USA.
authors.forename: Jamie A
authors.initials: JA
authors.lastname: O'Rourke
authors.forename: S Samuel
authors.initials: SS
authors.lastname: Yang
authors.forename: Susan S
authors.initials: SS
authors.lastname: Miller
authors.forename: Bruna
authors.initials: B
authors.lastname: Bucciarelli
authors.forename: Junqi
authors.initials: J
authors.lastname: Liu
authors.forename: Ariel
authors.initials: A
authors.lastname: Rydeen
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Bozsoki
authors.forename: Claudia
authors.initials: C
authors.lastname: Uhde-Stone
authors.forename: Zheng Jin
authors.initials: ZJ
authors.lastname: Tu
authors.forename: Deborah
authors.initials: D
authors.lastname: Allan
authors.forename: John W
authors.initials: JW
authors.lastname: Gronwald
authors.forename: Carroll P
authors.initials: CP
authors.lastname: Vance
mesh_terms: D000064:Acclimatization / Q000235:genetics*; D016000:Cluster Analysis; D017753:Ecosystem; D020869:Gene Expression Profiling / Q000379:methods; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D031297:Lupinus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010088:Oxidoreductases / Q000235:genetics; D010710:Phosphates / Q000378:metabolism / Q000494:pharmacology*; D010758:Phosphorus / Q000378:metabolism / Q000494:pharmacology*; D010940:Plant Proteins / Q000145:classification / Q000235:genetics; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D012987:Soil / Q000737:chemistry; D059467:Transcriptome / Q000187:drug effects*
chemical_list: D035683:MicroRNAs; D010710:Phosphates; D010940:Plant Proteins; D012987:Soil; D010758:Phosphorus; D010088:Oxidoreductases; C019984:cytokinin oxidase