title: Inactivation of Chikungunya virus by 1,5 iodonapthyl azide.
abstract: BACKGROUND
  Chikungunya virus (CHIKV) is an arthropod borne alphavirus of the family Togaviridae. CHIKV is a reemerging virus for which there is no safe prophylactic vaccine. A live attenuated strain of CHIKV, CHIK181/25, was previously demonstrated to be highly immunogenic in humans, however, it showed residual virulence causing transient arthralgia.
  
  
  RESULTS
  In this study, we demonstrate the complete inactivation of CHIKV181/25 by 1,5 iodonapthyl azide (INA). No cytopathic effect and virus replication was observed in cells infected with the INA-inactivated CHIKV. However, a reduction in the INA-inactivated CHIK virus-antibody binding capacity was observed by western blot analysis.
  
  
  CONCLUSIONS
  INA completely inactivated CHIKV and can further be explored for developing an inactivated-CHIKV vaccine.
authors.affiliation: Department of Pathology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA.
authors.forename: Anuj
authors.initials: A
authors.lastname: Sharma
authors.forename: Paridhi
authors.initials: P
authors.lastname: Gupta
authors.forename: Radha K
authors.initials: RK
authors.lastname: Maheshwari
mesh_terms: D000818:Animals; D001386:Azides / Q000494:pharmacology*; D002460:Cell Line; D002646:Chikungunya virus / Q000187:drug effects* / Q000235:genetics; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D012367:RNA, Viral; D038301:Virus Inactivation / Q000187:drug effects*
chemical_list: D001386:Azides; D012367:RNA, Viral; C015298:iodonaphthylazide