title: Regulating specific growth factor signaling using immobilized branched ligands.
abstract: VEGF-binding peptide ligands are incorporated into hydrogel microspheres and reduce the amount of growth factor in solution. VEGF binding affinity is enhanced by creating ligands with a dimer structure. The spheres are able to knock down VEGF-mediated HUVEC growth and reduce calcium signaling. The binding interaction is reversible, allowing the spheres to be used as a VEGF delivery vehicle.
authors.affiliation: Departments of Biomedical Engineering and Orthopedics & Rehabilitation, University of Wisconsin, 5009 Wisconsin Institutes of Medical Research, 1111 Highland Ave., Madison, WI 53705, USA.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Toepke
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Impellitteri
authors.forename: Sheeny K
authors.initials: SK
authors.lastname: Lan Levengood
authors.forename: Derek S
authors.initials: DS
authors.lastname: Boeldt
authors.forename: Ian M
authors.initials: IM
authors.lastname: Bird
authors.forename: William L
authors.initials: WL
authors.lastname: Murphy
mesh_terms: D002478:Cells, Cultured; D004337:Drug Carriers / Q000737:chemistry*; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D008024:Ligands; D010455:Peptides / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*
chemical_list: D004337:Drug Carriers; D036341:Intercellular Signaling Peptides and Proteins; D008024:Ligands; D010455:Peptides