title: [Multiplex real-time PCR method for rapid detection of Marburg virus and Ebola virus].
abstract: OBJECTIVE
  Marburg virus and Ebola virus are acute infections with high case fatality rates. A rapid, sensitive detection method was established to detect Marburg virus and Ebola virus by multiplex real-time fluorescence quantitative PCR.
  
  
  METHODS
  Designing primers and Taqman probes from highly conserved sequences of Marburg virus and Ebola virus through whole genome sequences alignment, Taqman probes labeled by FAM and Texas Red, the sensitivity of the multiplex real-time quantitative PCR assay was optimized by evaluating the different concentrations of primers and Probes.
  
  
  RESULTS
  We have developed a real-time PCR method with the sensitivity of 30.5 copies/microl for Marburg virus positive plasmid and 28.6 copies/microl for Ebola virus positive plasmids, Japanese encephalitis virus, Yellow fever virus, Dengue virus were using to examine the specificity.
  
  
  CONCLUSIONS
  The Multiplex real-time PCR assays provide a sensitive, reliable and efficient method to detect Marburg virus and Ebola virus simultaneously.
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing 100123, China.+
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.forename: Lin
authors.initials: L
authors.lastname: Bai
authors.forename: Kong-Xin
authors.initials: KX
authors.lastname: Hu
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Yang
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Hu
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
mesh_terms: D029043:Ebolavirus / Q000235:genetics* / Q000302:isolation & purification; D018702:Filoviridae Infections / Q000175:diagnosis / Q000821:virology*; D019142:Hemorrhagic Fever, Ebola / Q000175:diagnosis / Q000821:virology*; D006801:Humans; D029024:Marburgvirus / Q000235:genetics* / Q000302:isolation & purification; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*