title: Delivery of recombinant vaccines against bovine herpesvirus type 1 gD and Babesia bovis MSA-2c to mice using liposomes derived from egg yolk lipids.
abstract: Liposomes prepared from total egg yolk lipid extracts were used to deliver experimental DNA vaccines to mice consisting of pCI-neo plasmids encoding bovine herpesvirus type 1 (BoHV-1) gD or Babesia bovis MSA-2c. A significantly higher proportion of mice in the B. bovis MSA-2c group, but not those in the BoHV-1 gD group, developed detectable immunoglobulin G responses when vaccinated with liposome encapsulated DNA in comparison with mice vaccinated with naked DNA. In both groups, antibody titres were similar between mice vaccinated with liposome encapsulated DNA and naked DNA.
authors.affiliation: Institute of Pathobiology, Center of Veterinary and Agronomic Research (CICVyA), INTA, Los Reseros y Nicolas Repetto, s/n, 1686 Hurlingham, Argentina.
authors.forename: Anabel E
authors.initials: AE
authors.lastname: Rodriguez
authors.forename: Patricia
authors.initials: P
authors.lastname: Zamorano
authors.forename: Silvina
authors.initials: S
authors.lastname: Wilkowsky
authors.forename: Florencia
authors.initials: F
authors.lastname: Torrá
authors.forename: Lucas
authors.initials: L
authors.lastname: Ferreri
authors.forename: Mariana
authors.initials: M
authors.lastname: Dominguez
authors.forename: Mónica
authors.initials: M
authors.lastname: Florin-Christensen
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan; D000914:Antibodies, Viral; D000953:Antigens, Protozoan / Q000276:immunology; D016793:Babesia bovis / Q000276:immunology*; D001404:Babesiosis / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary; D004530:Egg Yolk / Q000737:chemistry*; D005260:Female; D006566:Herpesviridae Infections / Q000517:prevention & control; D007242:Herpesvirus 1, Bovine / Q000145:classification* / Q000276:immunology*; D008081:Liposomes / Q000737:chemistry*; D008297:Male; D008565:Membrane Proteins / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D015800:Protozoan Proteins / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000914:Antibodies, Viral; D000953:Antigens, Protozoan; D008081:Liposomes; C476548:MSA-2 protein, Babesia bovis; D008565:Membrane Proteins; D015800:Protozoan Proteins; D014764:Viral Proteins; D014765:Viral Vaccines