title: Bacterial extracellular polymeric substances and their effect on settlement of zoospore of Ulva fasciata.
abstract: The extracellular polymeric substances (EPSs) secreted by Bacillus flexus (GU592213) were estimated to have the molecular weight of approximately 1528 and 33,686 kDa with the elemental composition of Na, P, Mg, C, O, Cl and S. The (1)H NMR and FT-IR analysis of EPS confirmed the presence of different aliphatic and aromatic groups. The EPS was amorphous in nature with an average particle size of 13.969 μm (d 0.5) and roughness of 193 nm. The GC-MS analysis has revealed different monosaccharides such as fucose, ribose, xylose, galactose, mannose and glucose. Oligo and polysaccharides were detected with MALDI TOF-TOF MS. The bacterial EPS for the first time tested as a natural substratum for settle of zoospores of Ulva fasciata by incubating for various durations ranging from 2h to 48 h. The zoospore settlement on EPS coated cover slips progressively increased with incubation time in axenic cultures over controls. The EPS, thus investigated in this study was found to facilitate the primary settlement of spores that play crucial role in recruitment of macroalgal communities in coastal environment including intertidal regions.
authors.affiliation: Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 364 002, India.
authors.forename: Ravindra Pal
authors.initials: RP
authors.lastname: Singh
authors.forename: Mahendra K
authors.initials: MK
authors.lastname: Shukla
authors.forename: Avinash
authors.initials: A
authors.lastname: Mishra
authors.forename: C R K
authors.initials: CR
authors.lastname: Reddy
authors.forename: Bhavanath
authors.initials: B
authors.lastname: Jha
mesh_terms: D001407:Bacillus / Q000737:chemistry* / Q000254:growth & development; D001704:Biopolymers / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D005110:Extracellular Space / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D018625:Microscopy, Atomic Force; D008970:Molecular Weight; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017550:Spectroscopy, Fourier Transform Infrared; D044445:Ulva / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D001704:Biopolymers