title: Investigation of stable and transient protein-protein interactions: Past, present, and future.
abstract: This article presents an overview of the literature and a review of recent advances in the analysis of stable and transient protein-protein interactions (PPIs) with a focus on their function within cells, organs, and organisms. The significance of PTMs within the PPIs is also discussed. We focus on methods to study PPIs and methods of detecting PPIs, with particular emphasis on electrophoresis-based and MS-based investigation of PPIs, including specific examples. The validation of PPIs is emphasized and the limitations of the current methods for studying stable and transient PPIs are discussed. Perspectives regarding PPIs, with focus on bioinformatics and transient PPIs are also provided.
authors.affiliation: Biochemistry & Proteomics Group, Department of Chemistry & Biomolecular Science, Clarkson University, Potsdam, NY 13699, USA.
authors.forename: Armand G
authors.initials: AG
authors.lastname: Ngounou Wetie
authors.forename: Izabela
authors.initials: I
authors.lastname: Sokolowska
authors.forename: Alisa G
authors.initials: AG
authors.lastname: Woods
authors.forename: Urmi
authors.initials: U
authors.lastname: Roy
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Loo
authors.forename: Costel C
authors.initials: CC
authors.lastname: Darie
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D006801:Humans; D046912:Multiprotein Complexes / Q000302:isolation & purification / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000302:isolation & purification / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D046912:Multiprotein Complexes; D020543:Proteome