title: Mouse cytomegalovirus egress protein pM50 interacts with cellular endophilin-A2.
abstract: The herpesvirus replication cycle comprises maturation processes in the nucleus and cytoplasm of the infected cells. After their nuclear assembly viral capsids translocate via primary envelopment towards the cytoplasm. This event is mediated by the nuclear envelopment complex, which is composed by two conserved viral proteins belonging to the UL34 and UL31 protein families. Here, we generated recombinant viruses, which express affinity-tagged pM50 and/or pM53, the pUL34 and pUL31 homologues of the murine cytomegalovirus. We extracted pM50- and pM53-associated protein complexes from infected cells and analysed their composition after affinity purification by mass spectrometry. We observed reported interaction partners and identified new putative protein-protein interactions for both proteins. Endophilin-A2 was observed as the most prominent cellular partner of pM50. We found that endophilin-A2 binds to pM50 directly, and this interaction seems to be conserved in the pUL34 family.
authors.affiliation: Max von Pettenkofer-Institut, Ludwig-Maximilians-Universität München, Pettenkoferstr. 9a, 80336 Munich, Germany.
authors.forename: Frederic
authors.initials: F
authors.lastname: Lemnitzer
authors.forename: Verena
authors.initials: V
authors.lastname: Raschbichler
authors.forename: Dominika
authors.initials: D
authors.lastname: Kolodziejczak
authors.forename: Lars
authors.initials: L
authors.lastname: Israel
authors.forename: Axel
authors.initials: A
authors.lastname: Imhof
authors.forename: Susanne M
authors.initials: SM
authors.lastname: Bailer
authors.forename: Ulrich
authors.initials: U
authors.lastname: Koszinowski
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Ruzsics
mesh_terms: D000217:Acyltransferases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D003600:Cytosol / Q000378:metabolism / Q000821:virology; D015870:Gene Expression; D054884:Host-Pathogen Interactions; D013058:Mass Spectrometry; D051379:Mice; D018146:Muromegalovirus / Q000502:physiology*; D050506:Mutant Chimeric Proteins / Q000235:genetics / Q000378:metabolism*; D009685:Nuclear Envelope / Q000378:metabolism / Q000821:virology; D011485:Protein Binding; D025941:Protein Interaction Mapping; D034741:RNA, Small Interfering / Q000235:genetics; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D057074:Virus Release
chemical_list: D050506:Mutant Chimeric Proteins; D034741:RNA, Small Interfering; D014764:Viral Proteins; D000217:Acyltransferases; C018764:2-acylglycerophosphate acyltransferase