title: Determination method for p-Phenylazoaniline and 2-methyl-4-(2-tolylazo)aniline in workplace air by high-performance liquid chromatography.
abstract: OBJECTIVE
  The purpose of this research was to develop a method for the simultaneous determination of p-Phenylazoaniline (also called 4-aminoazobenzene, AAB) and 2-methyl-4-(2-tolylazo)aniline (also called o-aminoazotoluene, AAT) in workplace air for risk assessment.
  
  
  METHODS
  The characteristics of the proposed method, such as recovery, limit of quantitation, reproducibility and storage stability of the samples were examined.
  
  
  RESULTS
  An air sampling cassette containing two sulfuric acid-treated glass fiber filters was chosen as the sampler. The AAB and AAT were extracted from the sampler filters by methanol and then analyzed by a high-performance liquid chromatograph equipped with a photo-diode array detector. The overall recoveries from spiked samplers were 77-98 and 85-98% for AAB and AAT, respectively. The recovery after 5 days of storage in a refrigerator exceeded 96%. The overall limits of quantitation were 5.00 and 2.50 μg/sample for AAB and AAT, respectively. The relative standard deviations, which represent the overall reproducibility defined as precision, were 0.6-1.8 and 0.5-2.2% for AAB and AAT, respectively.
  
  
  CONCLUSIONS
  The proposed method enables 4-h personal exposure monitoring of AAB and AAT at concentrations of 21 to 2,000 μg/m3 for AAB and 10 to 2,000 μg/m3 for AAT, respectively. The proposed method is useful for estimating worker exposure to AAB and AAT.
authors.affiliation: Osaka Occupational Health Service Center, Japan Industrial Safety and Health Association, Japan. a-takeuchi@jisha.or.jp
authors.forename: Akito
authors.initials: A
authors.lastname: Takeuchi
authors.forename: Akira
authors.initials: A
authors.lastname: Jukurogi
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Kaifuku
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Natsumeda
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Ota
authors.forename: Shu
authors.initials: S
authors.lastname: Yamada
authors.forename: Kimiaki
authors.initials: K
authors.lastname: Sumino
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Kanno
mesh_terms: D000395:Air Pollutants, Occupational / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring / Q000379:methods*; D006801:Humans; D016273:Occupational Exposure / Q000032:analysis*; D016272:Occupational Health; D009762:o-Aminoazotoluene / Q000737:chemistry*; D010128:p-Aminoazobenzene / Q000737:chemistry*
chemical_list: D000395:Air Pollutants, Occupational; D010128:p-Aminoazobenzene; D009762:o-Aminoazotoluene