title: Lactate dehydrogenase, catalase, and superoxide dismutase in tumor tissue of breast cancer patients in respect to mammographic findings.
abstract: Lactate dehydrogenase (LDH), marker of anaerobic metabolism, is associated with highly invasive and metastatic breast cancer. Novel studies show that increased anaerobic metabolism (LDH), as well as activity of antioxidative enzymes (superoxide dismutase (SOD) and catalase (CAT)), is correlated with higher mammographic density, as known predictor of breast cancer risk. In this study, we measured LDH, MDH, and SOD activity in tumor and adjacent tissues of breast cancer patients by spectrophotometric assay. Mammograms were evaluated according to the American College of Radiology Breast Imaging Reporting and Data system. Mammographically dense breast tissue is associated with higher activity of LDH in tumor tissue of breast cancer patients. Moreover, patients with masses have significantly higher activity of LDH compared to patients with focal asymmetries or architectural distortion. Patients with spiculated mass margin had higher activity of LDH compared to patients with focal asymmetries or architectural distortion. Activity of LDH in patients significantly increases, while activity of CAT significantly decreases with the increase of BIRADS category. These results suggest that the association of activity of LDH and CAT in tumor tissue with mammographic characteristics could help in defining aggressive breast cancers.
authors.affiliation: Department of Radiation Oncology and Diagnostics, Institute of Oncology and Radiology of Serbia, Belgrade, Serbia. stankovics@dls.ncrc.ac.rs
authors.forename: Sandra
authors.initials: S
authors.lastname: Radenkovic
authors.forename: Zorica
authors.initials: Z
authors.lastname: Milosevic
authors.forename: Gordana
authors.initials: G
authors.lastname: Konjevic
authors.forename: Katarina
authors.initials: K
authors.lastname: Karadzic
authors.forename: Branislav
authors.initials: B
authors.lastname: Rovcanin
authors.forename: Marko
authors.initials: M
authors.lastname: Buta
authors.forename: Kristina
authors.initials: K
authors.lastname: Gopcevic
authors.forename: Vladimir
authors.initials: V
authors.lastname: Jurisic
mesh_terms: D000368:Aged; D001943:Breast Neoplasms / Q000000981:diagnostic imaging / Q000201:enzymology* / Q000473:pathology; D002374:Catalase / Q000378:metabolism*; D005260:Female; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008207:Lymphatic Metastasis; D008327:Mammography; D008875:Middle Aged; D009367:Neoplasm Staging; D020033:Protein Isoforms / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D020033:Protein Isoforms; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D013482:Superoxide Dismutase