title: Effect of extenders on sperm mitochondrial membrane, plasma membrane and sperm kinetics during liquid storage of canine semen at 5°C.
abstract: The objective of this study was to evaluate the effect of addition of egg yolk (EY) or soy lecithin (SL) based extenders on dog sperm parameters during 10days storage at 5°C. Four ejaculates of pooled semen from three Mongrel dogs were divided into three aliquots and extended to a final concentration of 200 million sperm/mL using following non-commercial extenders: egg yolk extender (EYE) group - semen+20% Tris-EYE; soy lecithin (SL) group 1 - semen+SL extender with 0.04% SL; SL group 2 - semen+SL extender 0.4% SL. The extended semen samples were stored at 5°C and were evaluated for sperm mitochondrial membrane potential (MMP), plasma membrane integrity (%PMI) and computer assisted sperm analyzer (CASA) sperm motility parameters on days 0, 1, 2, 3, 4, 7 and 10. The extender, days of storage and extender×days of storage affected the MMP, %PMI and CASA sperm motility parameters (P<0.05). The addition of 0.4% SL extender reduced the speed of deterioration of sperm parameters evaluated in this study compared to EY and 0.04% SL extenders (P<0.05). In conclusion, the addition of 0.4% SL extender enhanced sperm preservation based on the variables evaluated in the present study compared to EYE and 0.04% SL extenders and plausibly preserves sperm quality longer.
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USA. ramkasi@vetmed.wsu.edu
authors.forename: Vanmathy R
authors.initials: VR
authors.lastname: Kasimanickam
authors.forename: Ramanathan K
authors.initials: RK
authors.lastname: Kasimanickam
authors.forename: Mushtaq A
authors.initials: MA
authors.lastname: Memon
authors.forename: Harmon A
authors.initials: HA
authors.lastname: Rogers
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000187:drug effects*; D015925:Cryopreservation / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004285:Dogs; D008297:Male; D051336:Mitochondrial Membranes / Q000187:drug effects; D012662:Semen Preservation / Q000662:veterinary*; D013048:Specimen Handling; D013081:Sperm Motility / Q000187:drug effects / Q000502:physiology; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects* / Q000502:physiology
chemical_list: D003451:Cryoprotective Agents