title: Genetic analysis of heterokaryons in search of active micronuclear genes in stomatogenesis of Paramecium tetraurelia.
abstract: An experimental protocol to identify active micronuclear loci is described. The strategy is to construct heterokaryons, with micronucleus and macronucleus of different genotypes (mutant and wild-type) by micronuclear transplantation, and to assess whether the phenotype of the heterokaryons conforms to the genotype of the micronucleus, or the macronucleus. An appropriate gene marker is employed as a control against false interpretations resulting from transplantation and regeneration of a portion of the donor's macronucleus, and also from abnormality of the transplanted micronucleus. This analysis shows that the sh1 gene which determines the length of the oral apparatus is active in the macronucleus.
authors.affiliation: Zoology Department, University of Hong Kong, Hong Kong.
authors.forename: L W
authors.initials: LW
authors.lastname: Tam
authors.forename: S
authors.initials: S
authors.lastname: F Ng