title: Process quality engineering for bioreactor-driven manufacturing of tissue-engineered constructs for bone regeneration.
abstract: The incorporation of Quality-by-Design (QbD) principles in tissue-engineering bioprocess development toward clinical use will ensure that manufactured constructs possess prerequisite quality characteristics addressing emerging regulatory requirements and ensuring the functional in vivo behavior. In this work, the QbD principles were applied on a manufacturing process step for the in vitro production of osteogenic three-dimensional (3D) hybrid scaffolds that involves cell matrix deposition on a 3D titanium (Ti) alloy scaffold. An osteogenic cell source (human periosteum-derived cells) cultured in a bioinstructive medium was used to functionalize regular Ti scaffolds in a perfusion bioreactor, resulting in an osteogenic hybrid carrier. A two-level three-factor fractional factorial design of experiments was employed to explore a range of production-relevant process conditions by simultaneously changing value levels of the following parameters: flow rate (0.5-2 mL/min), cell culture duration (7-21 days), and cell-seeding density (1.5×10(3)-3×10(3) cells/cm(2)). This approach allowed to evaluate the individual impact of the aforementioned process parameters upon key quality attributes of the produced hybrids, such as collagen production, mineralization level, and cell number. The use of a fractional factorial design approach helped create a design space in which hybrid scaffolds of predefined quality attributes may be robustly manufactured while minimizing the number of required experiments.
authors.affiliation: Laboratory for Skeletal Development and Joint Disorders, Katholieke Universiteit Leuven, Leuven, Belgium.
authors.forename: Ioannis
authors.initials: I
authors.lastname: Papantoniou Ir
authors.forename: Yoke Chin
authors.initials: YC
authors.lastname: Chai
authors.forename: Frank P
authors.initials: FP
authors.lastname: Luyten
authors.forename: Jan
authors.initials: J
authors.lastname: Schrooten Ir
mesh_terms: D000497:Alloys; D019149:Bioreactors; D001861:Bone Regeneration; D002113:Calcification, Physiologic; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D005109:Extracellular Matrix / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D010012:Osteogenesis; D010521:Periosteum / Q000166:cytology / Q000378:metabolism; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D014025:Titanium / Q000737:chemistry*
chemical_list: D000497:Alloys; C031462:titanium alloy (TiAl6V4); D003094:Collagen; D014025:Titanium