title: Application of OECD Guideline 423 in assessing the acute oral toxicity of moniliformin.
abstract: Moniliformin is a Fusarium mycotoxin highly prevalent in grains and grain-based products worldwide. In this study, the acute oral toxicity of moniliformin was assessed in Sprague-Dawley male rats according to OECD Guideline 423 with a single-dose exposure. Clinical observations and histopathological changes were recorded together with the excretion of moniliformin via urine and feces, utilizing a novel liquid chromatography-mass spectrometry method. According to our study, moniliformin is acutely toxic to rats with a rather narrow range of toxicity. Moniliformin can be classified into category 2 (LD(50) cut-off value 25 mg/kg b.w.), according to the Globally Harmonized System for the classification of chemicals. The clinical observations included muscular weakness, respiratory distress and heart muscle damage. Pathological findings confirmed that heart is the main target tissue of acute moniliformin toxicity. A significant proportion (about 38%) of the administered moniliformin was rapidly excreted in urine in less than 6 h. However, the toxicokinetics of the majority of the administered dose still requires clarification, as the total excretion was only close to 42%. Considering the worldwide occurrence of moniliformin together with its high acute toxicity, research into the subchronic toxicity is of vital importance to identify the possible risk in human/animal health.
authors.affiliation: Finnish Food Safety Authority, Chemistry and Toxicology Research Unit, Finland. martina.jonsson@evira.fi
authors.forename: Martina
authors.initials: M
authors.lastname: Jonsson
authors.forename: Marika
authors.initials: M
authors.lastname: Jestoi
authors.forename: Alexis V
authors.initials: AV
authors.lastname: Nathanail
authors.forename: Ulla-Maija
authors.initials: UM
authors.lastname: Kokkonen
authors.forename: Marjukka
authors.initials: M
authors.lastname: Anttila
authors.forename: Pertti
authors.initials: P
authors.lastname: Koivisto
authors.forename: Pirkko
authors.initials: P
authors.lastname: Karhunen
authors.forename: Kimmo
authors.initials: K
authors.lastname: Peltonen
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002853:Chromatography, Liquid; D003503:Cyclobutanes / Q000633:toxicity* / Q000652:urine; D004305:Dose-Response Relationship, Drug; D002523:Edible Grain / Q000737:chemistry / Q000382:microbiology; D005243:Feces / Q000737:chemistry / Q000382:microbiology; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D005670:Fusarium / Q000378:metabolism; D017408:Guidelines as Topic; D007928:Lethal Dose 50; D008297:Male; D013058:Mass Spectrometry; D009183:Mycotoxins / Q000032:analysis / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D023382:Toxicity Tests, Acute / Q000379:methods*
chemical_list: D003503:Cyclobutanes; D009183:Mycotoxins; C008539:moniliformin