title: Colorimetric viral detection based on sialic acid stabilized gold nanoparticles.
abstract: Sialic acid reduced and stabilized gold nanoparticles (d=20.1±1.8 nm) were synthesized by a simple one-pot, green method without chemically modifying sialic acid for colorimetric detection of influenza virus. The gold nanoparticles showed target-specific aggregation with viral particles via hemagglutinin-sialic acid binding. A linear correlation was observed between the change in optical density and dilution of chemically inactivated influenza B/Victoria and influenza B/Yamagata. Virus dilution (hemagglutination assay titer, 512) of 0.156 vol% was readily detected. The upper limit of the linearity can be extended with the use of more sialic acid-gold nanoparticles.
authors.affiliation: Department of Chemistry, Biochemistry & Microbiology, University of Cincinnati, Cincinnati, OH 45221, USA.
authors.forename: Changwon
authors.initials: C
authors.lastname: Lee
authors.forename: Marsha A
authors.initials: MA
authors.lastname: Gaston
authors.forename: Alison A
authors.initials: AA
authors.lastname: Weiss
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
mesh_terms: D003124:Colorimetry; D006046:Gold / Q000737:chemistry*; D006388:Hemagglutinins / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D019158:N-Acetylneuraminic Acid / Q000138:chemical synthesis / Q000737:chemistry; D009975:Orthomyxoviridae / Q000302:isolation & purification*; D014771:Virion / Q000302:isolation & purification*
chemical_list: D006388:Hemagglutinins; D006046:Gold; D019158:N-Acetylneuraminic Acid