title: Adventitious Bud Formation from Bulb-scale Explants of Lilium speciosum Thunb. in vitro Production of ethane and ethylene.
abstract: We describe the influence of NAA, temperature, wounding, and AVG, an inhibitor of ethylene biosynthesis, on the production of ethylene and ethane by bud-regenerating bulb-scale explants of Lilium speciosum Thunb., cv. Rubrum nr. 10, in vitro. The production rates of the two hydrocarbon gases depended on culture stage and conditions, and their changes were opposite. An initial ethane production decreased at increasing ethylene evolution. The exponential increase of this evolution during the first 1-2 weeks was followed by a decline during subsequent bud differentiation. Bud formation was proportional to the maximum ethylene production. We conclude that the number of regenerated plantlets per explant was closely related to the speed of recovery and the measure of ethylene production during the initial period of membrane repair.
authors.affiliation: Bulb Research Centre, 2160 AB Lisse, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: van Aartrijk
authors.forename: G J
authors.initials: GJ
authors.lastname: Blom-Barnhoorn
authors.forename: J
authors.initials: J
authors.lastname: Bruinsma