title: Wood pulp as an immobilization matrix for the continuous production of isopropanol and butanol.
abstract: The study was focused on developing a continuous method to produce an alcohol mixture suitable to be used as a gasoline supplement. The immobilized column reactor with wood pulp fibers was successfully used for the continuous production of butanol and isopropanol using Clostridium beijerinckii DSM 6423. A sugar mixture (glucose, mannose, galactose, arabinose and xylose) representing lignocellulose hydrolysate was used as a substrate for the production of solvents. The effect of dilution rate on solvent production was studied during continuous operation. The maximum total solvent concentration of 11.99 g/l was obtained at a dilution rate of 0.16 h(-1). The maximum solvent productivity (5.58 g/l h) was obtained at a dilution rate of 1.5 h(-1). The maximum solvent yield of 0.45 g/g from sugar mixture was observed at 0.25 h(-1). The system will be further used for the solvent production using wood hydrolysate as a substrate.
authors.affiliation: Department of Biotechnology and Chemical Technology, Aalto University School of Chemical Technology, POB 16100, 00076 Aalto, Finland. shrikantraje1@rediffmail.com
authors.forename: Shrikant A
authors.initials: SA
authors.lastname: Survase
authors.forename: Adriaan
authors.initials: A
authors.lastname: van Heiningen
authors.forename: Tom
authors.initials: T
authors.lastname: Granström
mesh_terms: D020001:1-Butanol / Q000378:metabolism* / Q000600:supply & distribution; D019840:2-Propanol / Q000378:metabolism* / Q000600:supply & distribution; D019149:Bioreactors; D050260:Carbohydrate Metabolism; D046970:Clostridium beijerinckii / Q000378:metabolism*; D005285:Fermentation; D012997:Solvents / Q000378:metabolism; D014934:Wood / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D012997:Solvents; D020001:1-Butanol; D019840:2-Propanol