title: Biodegradation of di-n-butyl phthalate by a stable bacterial consortium, HD-1, enriched from activated sludge.
abstract: HD-1, a stable microbial consortium capable of mineralizing di-n-butyl phthalate (DBP), was developed from activated sludge. The dominant microorganisms in the consortium, Gordonia sp., Burkholderia sp. and Achromobacter sp., were identified by denaturing gradient gel electrophoresis (DGGE). The consortium could mineralize approximately 90% of 1200 mg/L DBP after 48 h of cultivation. The optimal DBP degradation conditions were 25-30 °C and pH 8.0-9.0. The addition of yeast (0.5 g/L), sodium acetate (0.5 g/L, 1.0 g/L), Brij 35 (0.2%, 0.5%, 1.0%), or Triton X-100 (0.2%) enhanced DBP degradation. The DBP degradation rate was influenced by the presence of dimethyl phthalate (DMP) and diethyl phthalate (DEP). Only one main intermediate, phthalic acid, could be monitored by gas chromatography-mass spectrometry (GC-MS) during the degradation process. The HD-1 consortium also utilized phenol, o-dihydroxybenzene as the sole carbon and energy source. The results indicate the consortium may represent a promising application for DBP bioremediation.
authors.affiliation: College of Life Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
authors.forename: Zhixing
authors.initials: Z
authors.lastname: He
authors.forename: Hailong
authors.initials: H
authors.lastname: Xiao
authors.forename: Lu
authors.initials: L
authors.lastname: Tang
authors.forename: Hang
authors.initials: H
authors.lastname: Min
authors.forename: Zhenmei
authors.initials: Z
authors.lastname: Lu
mesh_terms: D001420:Bacteria, Aerobic / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003993:Dibutyl Phthalate / Q000302:isolation & purification / Q000378:metabolism*; D059013:Microbial Consortia / Q000502:physiology*; D012722:Sewage / Q000382:microbiology*; D062065:Wastewater / Q000382:microbiology*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000378:metabolism*; D018508:Water Purification / Q000379:methods*
chemical_list: D003993:Dibutyl Phthalate; D012722:Sewage; D062065:Wastewater; D014874:Water Pollutants, Chemical