title: Differential tissular distribution of Litomosoides sigmodontis microfilariae between microfilaremic and amicrofilaremic mice following experimental infection.
abstract: Filariases are caused by onchocercid nematodes that are transmitted by arthropod vectors. More than 180 million people are infected worldwide. Mass drug administration has been set up in many endemic areas to control the parasite burden. Although very successful in limiting microfilarial load, transmission has not been completely interrupted in such areas. A proportion of infected patients with lymphatic filariasis or loiasis are known to be amicrofilaremic, as they do not present microfilariae in their bloodstream despite the presence of adult worms. A mirror status also exists in CBA/Ca mice infected with Litomosoides sigmodontis, the well-established model of filariasis. Using this model, the goal of this study was to determine if the kinetics of blood clearance of microfilariae differed between amicrofilaremic CBA/Ca mice and microfilaremic BALB/c mice. For this purpose, a qPCR approach was devised to detect microfilariae in different tissues, after a controlled inoculation of microfilariae. We showed that the rapid clearance of microfilariae from the pleural cavity or from the bloodstream of CBA/Ca mice was associated with a massive accumulation of first stage larvae in the lungs, liver and spleen.
authors.affiliation: UMR 7245 MCAM MNHN CNRS & UMR 7205 OSEB MNHN CNRS, Muséum National d'Histoire Naturelle, Paris Cedex, France.
authors.forename: T
authors.initials: T
authors.lastname: Bouchery
authors.forename: K
authors.initials: K
authors.lastname: Ehrhardt
authors.forename: E
authors.initials: E
authors.lastname: Lefoulon
authors.forename: W
authors.initials: W
authors.lastname: Hoffmann
authors.forename: O
authors.initials: O
authors.lastname: Bain
authors.forename: C
authors.initials: C
authors.lastname: Martin
mesh_terms: D000818:Animals; D001096:Arachnid Vectors / Q000469:parasitology; D005260:Female; D005368:Filariasis / Q000469:parasitology*; D005370:Filarioidea / Q000235:genetics / Q000502:physiology*; D008099:Liver / Q000469:parasitology; D008168:Lung / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008842:Microfilariae / Q000235:genetics / Q000502:physiology; D008925:Mites / Q000469:parasitology; D018512:Parasitemia / Q000469:parasitology; D035422:Pleural Cavity / Q000469:parasitology; D016133:Polymerase Chain Reaction / Q000592:standards; D015203:Reproducibility of Results; D013154:Spleen / Q000469:parasitology