title: Embryo Formation in Cell Cultures of the Legume Cyamopsis tetragonoloba (Guar).
abstract: Seedling tissues of Cyamopsis tetragonoloba (Guar) were cultured on various media to produce calli. These calli sometimes produced organs spontaneously; organogenesis was not controllable on any medium tried. Several calli were transferred to liquid shake cultures to form suspensions. One of these, along with one of the callus cultures, spontaneously turned embryogenic, producing large numbers of somatic embryoids. The embryoids could be removed and germinated on any of several media to plantlet stage, but degeneration of the root primordium precluded further development.
authors.affiliation: Crop Development Centre, University of Saskatchewan, Saskatoon, Sask., S7N 0W0, Canada.
authors.forename: A
authors.initials: A
authors.lastname: McHughen
authors.forename: M
authors.initials: M
authors.lastname: Swartz