title: Geographic distribution and genetic compatibility among six karyotypic forms of Anopheles peditaeniatus (Diptera: Culicidae) in Thailand.
abstract: Fifty-three isolines of Anopheles peditaeniatus were established from individual   wild-caught females collected from cow-baited traps in 17 provinces of Thailand. Three   types of X (X1, X2, X3) and 6 types of Y (Y1,Y2, Y3, Y4, Y5, Y6) chromosomes were determined   based on different amounts of major block(s) of heterochromatin. These sex chromosomes   comprised 6 karyotypic forms designated as Forms A (X3, Y1), B (X1, X2, X3, Y2), C (X3, Y3), D   (X1, X2, X3, Y4), E (X1, X2, X3,Y5) and F (X2, X3, Y6). Form F is a new metaphase karyotype   discovered in this study and is commonly found in all regions. Form A was found only in   Lampang province, whereas Form E is widespread throughout the country. Forms B, C and D   were obtained from the northern, northeastern, western and southern regions. Crossing   experiments among the 11 isoline colonies representing the 6 karyotypic forms of An.   peditaeniatus indicated genetic compatibility yielding viable progenies and complete synapsis   of salivary gland polytene chromosomes through to the F2-generations. The results suggested   the conspecific nature of these karyotypic forms which were further supported by very low   intraspecific variation (genetic distance = 0.000-0.003) of nucleotide sequences in ribosomal   DNA (ITS2) and mitochondrial DNA (COI and COII).
authors.affiliation: Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. atsaeung@mail.med.cmu.ac.th
authors.forename: A
authors.initials: A
authors.lastname: Saeung
authors.forename: V
authors.initials: V
authors.lastname: Baimai
authors.forename: S
authors.initials: S
authors.lastname: Thongsahuan
authors.forename: G S
authors.initials: GS
authors.lastname: Min
authors.forename: M H
authors.initials: MH
authors.lastname: Park
authors.forename: Y
authors.initials: Y
authors.lastname: Otsuka
authors.forename: W
authors.initials: W
authors.lastname: Maleewong
authors.forename: V
authors.initials: V
authors.lastname: Lulitanond
authors.forename: K
authors.initials: K
authors.lastname: Taai
authors.forename: W
authors.initials: W
authors.lastname: Choochote
mesh_terms: D000818:Animals; D000852:Anopheles / Q000145:classification / Q000235:genetics* / Q000254:growth & development*; D002417:Cattle; D003433:Crosses, Genetic; D005260:Female; D006570:Heterochromatin / Q000737:chemistry / Q000378:metabolism; D059785:Karyotype; D008297:Male; D008969:Molecular Sequence Data; D058974:Phylogeography; D017422:Sequence Analysis, DNA; D013785:Thailand; D014960:X Chromosome / Q000737:chemistry / Q000378:metabolism; D014998:Y Chromosome / Q000737:chemistry / Q000378:metabolism
chemical_list: D006570:Heterochromatin