title: A novel fed-batch process based on the biology of Aurantiochytrium sp. KRS101 for the production of biodiesel and docosahexaenoic acid.
abstract: The biology of Aurantiochytrium sp. KRS101 was thoroughly investigated to enhance its production of biodiesel and docosahexaenoic acid (DHA). Nutrients and salinity were optimized to prevent biomass loss due to cell rupture. Calculation of yield coefficients showed that nitrogen was mostly responsible for the early stage of cell growth or division, whereas carbon was necessary for the entire process of cell development, particularly cell enlargement during late stages. Using these distinctive yield coefficients, a modified fed-batch cultivation method was designed, resulting in increases in palmitic acid (PA) and DHA production of up to 137% and 29%, respectively. This modified fed-batch cultivation method, using appropriate supplies of nitrogen and carbon, may improve the yields of PA and DHA, thus expanding the biotechnological applications of Aurantiochytrium sp. KRS101.
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, 335 Gwahak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Kyochan
authors.initials: K
authors.lastname: Kim
authors.forename: Eun
authors.initials: E
authors.lastname: Jung Kim
authors.forename: Byung-Gon
authors.initials: BG
authors.lastname: Ryu
authors.forename: Soojung
authors.initials: S
authors.lastname: Park
authors.forename: Yoon-E
authors.initials: YE
authors.lastname: Choi
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Yang
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods*; D056804:Biofuels; D018533:Biomass; D002244:Carbon / Q000494:pharmacology; D004281:Docosahexaenoic Acids / Q000096:biosynthesis*; D005947:Glucose / Q000494:pharmacology; D009584:Nitrogen / Q000494:pharmacology; D019308:Palmitic Acid / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D058009:Stramenopiles / Q000166:cytology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
chemical_list: D056804:Biofuels; D004281:Docosahexaenoic Acids; D019308:Palmitic Acid; D012965:Sodium Chloride; D002244:Carbon; D005947:Glucose; D009584:Nitrogen