title: Highly potent bactericidal activity of porous metal-organic frameworks.
abstract: Recent outbreaks of bacterial infection leading to human fatalities have been a motivational force for us to develop antibacterial agents with high potency and long-term stability. A novel cobalt (Co) based metal-organic framework (MOF) was tested and shown to be highly effective at inactivating model microorganisms. Gram-negative bacteria, Escherichia coli (strains DH5alpha and XL1-Blue) were selected to determine the antibacterial activities of the Co MOF. In this MOF, the Co serves as a central element and an octa-topic carboxylate ligand, tetrakis [(3,5-dicarboxyphenyl)-oxamethyl] methane (TDM(8-) ) serves as a bridging linker. X-ray crystallographic studies indicate that Co-TDM crystallizes in tetragonal space group P$\overline 4$2(1) m with a porous 3D framework. The potency of the Co-TDM disinfectant was evaluated using a minimal bactericidal concentration (MBC) benchmark and was determined to be 10-15 ppm within a short incubation time period (<60 min). Compared with previous work using silver nanoparticles and silver-modified TiO(2)  nano- composites over the same time period, the MBC and effectiveness of Co-TDM are superior. Electron microscopy images indicate that the Co-TDM displayed distinctive grain boundaries and well-developed reticulates. The Co active sites rapidly catalyzed the lipid peroxidation, causing rupture of the bacterial membrane followed by inactivation, with 100% recycling and high persistence (>4 weeks). This MOF-based approach may lead to a new paradigm for MOF applications in diverse biological fields due to their inherent porous structure, tunable surface functional groups, and adjustable metal coordination environments.
authors.affiliation: Chemistry Department, Texas A&M University, College Station, TX 77843, USA.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Zhuang
authors.forename: Daqiang
authors.initials: D
authors.lastname: Yuan
authors.forename: Jian-Rong
authors.initials: JR
authors.lastname: Li
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Luo
authors.forename: Hong-Cai
authors.initials: HC
authors.lastname: Zhou
authors.forename: Sajid
authors.initials: S
authors.lastname: Bashir
authors.forename: Jingbo
authors.initials: J
authors.lastname: Liu
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D003035:Cobalt / Q000737:chemistry* / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology*; D009930:Organic Chemicals / Q000737:chemistry* / Q000494:pharmacology*; D016062:Porosity
chemical_list: D000900:Anti-Bacterial Agents; D009930:Organic Chemicals; D003035:Cobalt