title: Improved acid tolerance of Lactobacillus pentosus by error-prone whole genome amplification.
abstract: Acid tolerance of Lactobacillus pentosus ATCC 8041 was improved by error-prone amplification of its genomic DNA using random primers and Taq DNA polymerase. The resulting amplification products were transferred into wild-type L. pentosus by electroporation and the transformants were screened for growth on low-pH agar plates. After only one round of mutation, one mutant (MT3) was identified that was able to completely consume 20 g/L of glucose to produce lactic acid at a yield of 95% in 1L MRS medium at pH 3.8 within 36 h, whereas no growth or lactic acid production was observed for the wild-type strain under the same conditions. The acid tolerance of mutant MT3 remained genetically stable for at least 25 subcultures. Therefore, the error-prone whole genome amplification technique is a very powerful tool for improving phenotypes of this lactic acid bacterium and may also be applicable for other microorganisms.
authors.affiliation: Institute of Chemical and Engineering Sciences, Agency for Sciences, Technology and Research (A*STAR), 1 Pesek Road, Jurong Island, Singapore 627833, Singapore.
authors.forename: Lidan
authors.initials: L
authors.lastname: Ye
authors.forename: Hua
authors.initials: H
authors.lastname: Zhao
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.forename: Jin Chuan
authors.initials: JC
authors.lastname: Wu
mesh_terms: D000143:Acids / Q000494:pharmacology*; D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics*; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D016680:Genome, Bacterial / Q000235:genetics*; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D019344:Lactic Acid / Q000096:biosynthesis; D007778:Lactobacillus / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D009154:Mutation / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D000143:Acids; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase; D005947:Glucose