title: Circulating MicroRNAs as potential risk biomarkers for hematoma enlargement after intracerebral hemorrhage.
abstract: OBJECTIVE
  MicroRNAs have recently been shown to regulate the downstream bioprocesses of intracerebral hemorrhage. The aim of this study was to investigate whether miRNAs can be used as biomarkers to predict secondary hematoma enlargement (HE) in patients with ICH.
  
  
  METHODS
  Consecutively, 79 ICH patients admitted within 6 h of symptom onset and 30 healthy individuals were enrolled in this study. Whole-genome miRNA expression profiles were generated in 32 patients (HE/non-HE: 14/18). Representative differentially expressed miRNAs were measured in all cases (HE/non-HE: 30/49) and normal controls (n = 30) by real-time PCR.
  
  
  RESULTS
  Thirty miRNAs showed differential expressions in the plasma samples from patients with HE as compared with the non-HE controls. Compared to the hierarchical cluster analysis with all probes on microarray, all patients were separated into two main branches with only four exceptions by 30 differentially expressed miRNAs, improving the overall accuracy from 47.62 to 77.78% in the HE and 72.73 to 100% in the non-HE group. Further support vector machine (SVM) test can discriminate the two groups with 100% accuracy with 10 differentially expressed miRNAs.
  
  
  CONCLUSIONS
  We demonstrated that multiple miRNAs are differentially expressed in the plasma of ICH patients with or without HE and may serve as circulating biomarkers for predicting HE after ICH.
authors.affiliation: Department of Neurology, Beijing Tiantan Hospital Affiliated to Capital Medical University, Beijing, China.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Zheng
authors.forename: Yi-Long
authors.initials: YL
authors.lastname: Wang
authors.forename: Jin-Xi
authors.initials: JX
authors.lastname: Lin
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Xing-Quan
authors.initials: XQ
authors.lastname: Zhao
authors.forename: Gai-Fen
authors.initials: GF
authors.lastname: Liu
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiao
authors.forename: Wei-Kuan
authors.initials: WK
authors.lastname: Gu
authors.forename: David Z
authors.initials: DZ
authors.lastname: Wang
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D002543:Cerebral Hemorrhage / Q000150:complications*; D019295:Computational Biology; D005260:Female; D006406:Hematoma / Q000097:blood* / Q000209:etiology*; D006801:Humans; D018810:Magnetic Resonance Angiography; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism; D012189:Retrospective Studies; D059467:Transcriptome
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; D012333:RNA, Messenger