title: Generating embryonic stem cells from the inbred mouse strain DBA/2J, a model of glaucoma and other complex diseases.
abstract: Mouse embryonic stem (ES) cells are derived from the inner cell mass of blastocyst stage embryos and are used primarily for the creation of genetically engineered strains through gene targeting. While some inbred strains of mice are permissive to the derivation of embryonic stem cell lines and are therefore easily engineered, others are nonpermissive or recalcitrant. Genetic engineering of recalcitrant strain backgrounds requires gene targeting in a permissive background followed by extensive backcrossing of the engineered allele into the desired strain background. The inbred mouse strain DBA/2J is a recalcitrant strain that is used as a model of many human diseases, including glaucoma, deafness and schizophrenia. Here, we describe the generation of germ-line competent ES cell lines derived from DBA/2J mice. We also demonstrate the utility of DBA/2J ES cells with the creation of conditional knockout allele for Endothelin-2 (Edn2) directly on the DBA/2J strain background.
authors.affiliation: The Jackson Laboratory, Bar Harbor, Maine, United States of America.
authors.forename: Laura G
authors.initials: LG
authors.lastname: Reinholdt
authors.forename: Gareth R
authors.initials: GR
authors.lastname: Howell
authors.forename: Anne M
authors.initials: AM
authors.lastname: Czechanski
authors.forename: Danilo G
authors.initials: DG
authors.lastname: Macalinao
authors.forename: Katharine H
authors.initials: KH
authors.lastname: Macnicoll
authors.forename: Chyuan-Sheng
authors.initials: CS
authors.lastname: Lin
authors.forename: Leah Rae
authors.initials: LR
authors.lastname: Donahue
authors.forename: Simon W M
authors.initials: SW
authors.lastname: John
mesh_terms: D000483:Alleles; D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D003638:Deafness / Q000235:genetics; D004195:Disease Models, Animal; D053595:Embryonic Stem Cells / Q000166:cytology*; D019333:Endothelin-2 / Q000235:genetics; D005260:Female; D005818:Genetic Engineering / Q000379:methods; D005821:Genetic Techniques; D016678:Genome; D005901:Glaucoma / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008856:Microscopy, Fluorescence / Q000379:methods; D008957:Models, Genetic; D012559:Schizophrenia / Q000235:genetics
chemical_list: D003470:Culture Media; D019333:Endothelin-2