title: MiR-320a is downregulated in patients with myasthenia gravis and modulates inflammatory cytokines production by targeting mitogen-activated protein kinase 1.
abstract: Myasthenia gravis (MG) are T-cell dependent antibody-mediated autoimmune disorders, microRNAs are important regulators of human autoimmune disease pathogenesis. Here, we investigated the miRNAs expression profiles in MG for the first time and found that miR-320a was significantly downregulated in MG patients compared to normal healthy people. Meanwhile, pro-inflammatory cytokins in MG patients were overexpressed. Furthermore, we identified MAPK1 as a direct target of miR-320a. Downregulation of miR-320a induced the overexpression of pro-inflammatory cytokins through promoting COX-2 expression. This process was modulated by ERK/ NF-κB pathways. Taken together, our findings suggested that miR-320a could play a role in modulation of inflammatory cytokins production.
authors.affiliation: State Key Laboratory of Bioreactor Engineering & Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
authors.forename: Zhuoan
authors.initials: Z
authors.lastname: Cheng
authors.forename: Shaobo
authors.initials: S
authors.lastname: Qiu
authors.forename: Lin
authors.initials: L
authors.lastname: Jiang
authors.forename: Anle
authors.initials: A
authors.lastname: Zhang
authors.forename: Wenjing
authors.initials: W
authors.lastname: Bao
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Jianwen
authors.initials: J
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D018836:Inflammation Mediators; D008297:Male; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D008875:Middle Aged; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics* / Q000378:metabolism; D009157:Myasthenia Gravis / Q000235:genetics* / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D015398:Signal Transduction
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; C572916:MIRN320 microRNA, human; D035683:MicroRNAs; D016328:NF-kappa B; D019950:Mitogen-Activated Protein Kinase 1