title: Two-dimensional fluorescence difference gel electrophoresis analysis of Listeria monocytogenes submitted to a redox shock.
abstract: The influence of redox alteration on the growth and proteomic pattern of Listeria monocytogenes was investigated. A redox shock was induced in cultures by addition of 3mM ferricyanide (FeCN) and 6mM dithiothreitol (DTT) to increase or to decrease respectively the redox potential naturally occurring at the beginning of growth. In both conditions, the reducing and oxidizing redox shock had a strong influence, decreasing the maximum growth rate by half compared to a control culture. The proteomic analysis of L. monocytogenes performed by two-dimensional difference gel electrophoresis (2D-DIGE) exhibited twenty-three proteins differentially expressed (P<0.05), among these, many were oxidoreductases, and proteins involved in cellular metabolism (glycolysis, protein synthesis), detoxification (kat) or adhesion (Lmo1634).
authors.affiliation: INRA, Nantes, F-44307, France; LUNAM Université, ONIRIS, UMR1014 SECALIM, Nantes, F-44307, France.
authors.forename: Maria
authors.initials: M
authors.lastname: Ignatova
authors.forename: Blandine
authors.initials: B
authors.lastname: Guével
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Com
authors.forename: Nabila
authors.initials: N
authors.lastname: Haddad
authors.forename: Albert
authors.initials: A
authors.lastname: Rossero
authors.forename: Philippe
authors.initials: P
authors.lastname: Bogard
authors.forename: Hervé
authors.initials: H
authors.lastname: Prévost
authors.forename: Sandrine
authors.initials: S
authors.lastname: Guillou
mesh_terms: D002232:Carbocyanines; D004229:Dithiothreitol / Q000494:pharmacology*; D005292:Ferricyanides / Q000494:pharmacology*; D008089:Listeria monocytogenes / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D057825:Two-Dimensional Difference Gel Electrophoresis / Q000379:methods*
chemical_list: D002232:Carbocyanines; D005292:Ferricyanides; D004229:Dithiothreitol