title: Structured illumination microscopy for in-vivo human retinal imaging: a theoretical assessment.
abstract: Structured illumination microscopy applied to in-vivo retinal imaging has the potential to provide a low-cost and powerful diagnostic tool for retinal disease. In this paper the key parameters that affect performance in structured illumination ophthalmoscopy are studied theoretically. These include the number of images that need to be acquired in order to generate a sectioned image, which is affected by the non-stationary nature of the retina during acquisition, the choice of spatial frequency of the illuminating sinusoid, the effect of typical ocular aberrations on axial resolution and the nature of the sinusoidal pattern produced by the illumination system. The results indicate that structured illumination ophthalmoscopy can be a robust technique for achieving axial sectioning in retinal imaging without the need for complex optical systems.
authors.affiliation: Optometry and Visual Science, School of Health Sciences, City University London, EC1V 0HB, UK.
authors.forename: Sabah
authors.initials: S
authors.lastname: Chetty
authors.forename: Steve
authors.initials: S
authors.lastname: Gruppetta
mesh_terms: D000465:Algorithms; D003952:Diagnostic Imaging / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008027:Light; D008029:Lighting; D008853:Microscopy / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D015233:Models, Statistical; D019721:Ophthalmoscopes; D009887:Ophthalmoscopy / Q000379:methods; D055095:Optics and Photonics; D012160:Retina / Q000378:metabolism / Q000473:pathology / Q000502:physiology*