title: The coronatine toxin of Pseudomonas syringae is a multifunctional suppressor of Arabidopsis defense.
abstract: The phytotoxin coronatine (COR) promotes various aspects of Pseudomonas syringae virulence, including invasion through stomata, growth in the apoplast, and induction of disease symptoms. COR is a structural mimic of active jasmonic acid (JA) conjugates. Known activities of COR are mediated through its binding to the F-box-containing JA coreceptor CORONATINE INSENSITIVE1. By analyzing the interaction of P. syringae mutants with Arabidopsis thaliana mutants, we demonstrate that, in the apoplastic space of Arabidopsis, COR is a multifunctional defense suppressor. COR and the critical P. syringae type III effector HopM1 target distinct signaling steps to suppress callose deposition. In addition to its well-documented ability to suppress salicylic acid (SA) signaling, COR suppresses an SA-independent pathway contributing to callose deposition by reducing accumulation of an indole glucosinolate upstream of the activity of the PEN2 myrosinase. COR also suppresses callose deposition and promotes bacterial growth in coi1 mutant plants, indicating that COR may have multiple targets inside plant cells.
authors.affiliation: Department of Horticulture and Crop Science, Ohio State University, Columbus, Ohio 43210, USA.
authors.forename: Xueqing
authors.initials: X
authors.lastname: Geng
authors.forename: Jiye
authors.initials: J
authors.lastname: Cheng
authors.forename: Anju
authors.initials: A
authors.lastname: Gangadharan
authors.forename: David
authors.initials: D
authors.lastname: Mackey
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D017360:Arabidopsis / Q000276:immunology* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005936:Glucans / Q000378:metabolism; D005961:Glucosinolates / Q000378:metabolism; D054884:Host-Pathogen Interactions; D007192:Indenes / Q000378:metabolism*; D007211:Indoles / Q000378:metabolism; D009154:Mutation; D009699:N-Glycosyl Hydrolases / Q000235:genetics / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D010937:Plant Growth Regulators / Q000378:metabolism*; D057865:Plant Immunity; D044224:Pseudomonas syringae / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*; D020156:Salicylic Acid / Q000378:metabolism; D015398:Signal Transduction; D014774:Virulence
chemical_list: D000596:Amino Acids; D029681:Arabidopsis Proteins; C112279:COI1 protein, Arabidopsis; D003517:Cyclopentanes; D005936:Glucans; D005961:Glucosinolates; D007192:Indenes; D007211:Indoles; D054883:Oxylipins; D010937:Plant Growth Regulators; C058487:coronatine; C011006:jasmonic acid; C030374:indole; C048306:callose; D009699:N-Glycosyl Hydrolases; C505663:PEN2 protein, Arabidopsis; D020156:Salicylic Acid