title: Creation of a female rabbit model for intrauterine adhesions using mechanical and infectious injury.
abstract: BACKGROUND
  Intrauterine adhesions (IUA) are associated with secondary amenorrhea, infertility, and recurrent pregnancy loss. An IUA animal model would contribute to research on the pathogenesis and pathologic changes of IUA and the exploration of new treatments.
  
  
  METHODS
  Eighty female rabbits were randomly divided into five groups: mechanical injury (16), infectious injury (16), dual injury (16), experimental control (16), and normal (16). Three methods were applied to establish the model: uterine curettage, uterine cavity left alone, lipopolysaccharide surgical suture in place for 48 h, and suture retention for 48 h after curettage. A sterile surgical suture was left in the uterine cavity for 48 h in the experimental control group. Histologic changes were monitored at 0, 24, 48, and 72 h and 7, 14, and 28 d after operation.
  
  
  RESULTS
  The experiments revealed that endometrium injured by simple curettage or infection could be repaired. Although endometrial regeneration was severely impaired by dual injury, the ratio of the area with endometrial stromal fibrosis to total endometrial area significantly increased (P < 0.01), and the number of endometrial glands was significantly reduced (P < 0.01).
  
  
  CONCLUSIONS
  The method of dual injury can establish a stable rabbit IUA model.
authors.affiliation: Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Zhu
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Yuan-Li
authors.initials: YL
authors.lastname: He
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D008070:Lipopolysaccharides; D011817:Rabbits; D000267:Tissue Adhesions / Q000209:etiology / Q000473:pathology; D014591:Uterine Diseases / Q000209:etiology* / Q000473:pathology; D014599:Uterus / Q000293:injuries* / Q000473:pathology
chemical_list: D008070:Lipopolysaccharides