title: Proteomic analysis of "Moncada" mandarin leaves with contrasting fruit load.
abstract: A proteomic approach was used to know more about the molecular mechanism related to Citrus alternate bearing. To this end, we researched protein expression differences between on-crop and off-crop "Moncada" [Clementine 'Oroval' (Citrus clementina Hort ex Tanaka) x 'Kara' mandarin (Citrus unshiu Marc. x Citrus nobilis Lou.)] mandarin leaves. This variety usually shows a remarkable behaviour in alternate production. Samples were collected in the period during which the fruit affect flowering induction. From 2D DIGE gel, 110 spots were isolated: 43 showed increased expression in the off-crop samples compared to on-crop samples, while 67 showed increased expression in the on-crop samples against off-crop samples. These spots were identified by MALDI-MS or LC-MS-MS. According to the up-expressed proteins in off-crop leaves such as proteins related to nutrient reservoir activity or to the pentose phosphate pathway, the primary metabolism was more active in off-crop trees than in on-crop trees. In contrast, the proteins up-expressed in on-crop samples such as catalase were related to the oxidoreductase activity and, therefore, the redox state seemed different for off-crop and for on-crop leaves. Other proteins with unknown functions were isolated, which could be also related to the alternate bearing and to the flowering induction.
authors.affiliation: Instituto Agroforestal Mediterráneo, Universidad Politécnica de Valencia, C/Ingeniero Fausto Elio, E-46022 Valencia, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Muñoz-Fambuena
authors.forename: Carlos
authors.initials: C
authors.lastname: Mesejo
authors.forename: Manuel
authors.initials: M
authors.lastname: Agustí
authors.forename: Susana
authors.initials: S
authors.lastname: Tárraga
authors.forename: Domingo J
authors.initials: DJ
authors.lastname: Iglesias
authors.forename: Eduardo
authors.initials: E
authors.lastname: Primo-Millo
authors.forename: M Carmen
authors.initials: MC
authors.lastname: González-Mas
mesh_terms: D002957:Citrus / Q000378:metabolism*; D005638:Fruit / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics
chemical_list: D010940:Plant Proteins; D020543:Proteome