title: The bifunctional aldehyde-alcohol dehydrogenase controls ethanol and acetate production in Entamoeba histolytica under aerobic conditions.
abstract: By applying metabolic control analysis and inhibitor titration we determined the degree of control (flux control coefficient) of pyruvate:ferredoxin oxidoreductase (PFOR) and bifunctional aldehyde-alcohol dehydrogenase (ADHE) over the fluxes of fermentative glycolysis of Entamoeba histolytica subjected to aerobic conditions. The flux-control coefficients towards ethanol and acetate formation determined for PFOR titrated with diphenyleneiodonium were 0.07 and 0.09, whereas for ADHE titrated with disulfiram were 0.33 and -0.19, respectively. ADHE inhibition induced significant accumulation of glycolytic intermediates and lower ATP content. These results indicate that ADHE exerts significant flux-control on the carbon end-product formation of amoebas subjected to aerobic conditions.
authors.affiliation: Departamento de Bioquímica, Instituto Nacional de Cardiología Ignacio Chávez, México D.F. 14080, Mexico.
authors.forename: Erika
authors.initials: E
authors.lastname: Pineda
authors.forename: Rusely
authors.initials: R
authors.lastname: Encalada
authors.forename: Alfonso
authors.initials: A
authors.lastname: Olivos-García
authors.forename: Mario
authors.initials: M
authors.lastname: Néquiz
authors.forename: Rafael
authors.initials: R
authors.lastname: Moreno-Sánchez
authors.forename: Emma
authors.initials: E
authors.lastname: Saavedra
mesh_terms: D019342:Acetic Acid / Q000378:metabolism*; D000332:Aerobiosis; D000426:Alcohol Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000445:Aldehyde Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004221:Disulfiram / Q000494:pharmacology; D004748:Entamoeba histolytica / Q000187:drug effects / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D000431:Ethanol / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects; D007700:Kinetics; D053858:Metabolic Networks and Pathways; D009861:Onium Compounds / Q000494:pharmacology; D015800:Protozoan Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D050743:Pyruvate Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism; D053058:Trophozoites / Q000187:drug effects / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D009861:Onium Compounds; D015800:Protozoan Proteins; D000431:Ethanol; C007517:diphenyleneiodonium; D000426:Alcohol Dehydrogenase; D000445:Aldehyde Oxidoreductases; D050743:Pyruvate Synthase; D019342:Acetic Acid; D004221:Disulfiram