title: Hemocompatible control of sulfobetaine-grafted polypropylene fibrous membranes in human whole blood via plasma-induced surface zwitterionization.
abstract: In this work, the hemocompatibility of zwitterionic polypropylene (PP) fibrous membranes with varying grafting coverage of poly(sulfobetaine methacrylate) (PSBMA) via plasma-induced surface polymerization was studied. Charge neutrality of PSBMA-grafted layers on PP membrane surfaces was controlled by the low-pressure and atmospheric plasma treatment in this study. The effects of grafting composition, surface hydrophilicity, and hydration capability on blood compatibility of the membranes were determined. Protein adsorption onto the different PSBMA-grafted PP membranes from human fibrinogen solutions was measured by enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies. Blood platelet adhesion and plasma clotting time measurements from a recalcified platelet-rich plasma solution were used to determine if platelet activation depends on the charge bias of the grafted PSBMA layer. The charge bias of PSBMA layer deviated from the electrical balance of positively and negatively charged moieties can be well-controlled via atmospheric plasma-induced interfacial zwitterionization and was further tested with human whole blood. The optimized PSBMA surface graft layer in overall charge neutrality has a high hydration capability and keeps its original blood-inert property of antifouling, anticoagulant, and antithrmbogenic activities when it comes into contact with human blood. This work suggests that the hemocompatible nature of grafted PSBMA polymers by controlling grafting quality via atmospheric plasma treatment gives a great potential in the surface zwitterionization of hydrophobic membranes for use in human whole blood.
authors.affiliation: R&D Center for Membrane Technology and Department of Chemical Engineering, Chung Yuan University, Chung-Li, Taoyuan 320, Taiwan.
authors.forename: Sheng-Han
authors.initials: SH
authors.lastname: Chen
authors.forename: Yung
authors.initials: Y
authors.lastname: Chang
authors.forename: Kueir-Rarn
authors.initials: KR
authors.lastname: Lee
authors.forename: Ta-Chin
authors.initials: TC
authors.lastname: Wei
authors.forename: Akon
authors.initials: A
authors.lastname: Higuchi
authors.forename: Feng-Ming
authors.initials: FM
authors.lastname: Ho
authors.forename: Chia-Chun
authors.initials: CC
authors.lastname: Tsou
authors.forename: Hsin-Tsung
authors.initials: HT
authors.lastname: Ho
authors.forename: Juin-Yih
authors.initials: JY
authors.lastname: Lai
mesh_terms: D000327:Adsorption; D000925:Anticoagulants / Q000737:chemistry / Q000494:pharmacology; D001622:Betaine / Q000031:analogs & derivatives* / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D001798:Blood Proteins / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008567:Membranes, Artificial; D008689:Methacrylates / Q000737:chemistry; D010949:Plasma / Q000737:chemistry*; D010973:Platelet Adhesiveness / Q000187:drug effects; D011126:Polypropylenes / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000925:Anticoagulants; D001672:Biocompatible Materials; D001798:Blood Proteins; D008567:Membranes, Artificial; D008689:Methacrylates; D011126:Polypropylenes; C521589:sulfobetaine methacrylate polymer; D001622:Betaine; C483727:sulfobetaine