title: Effects of warm ischemia time on biliary injury in rat liver transplantation.
abstract: OBJECTIVE
  To investigate the effect of different secondary warm ischemia time (SWIT) on bile duct injury in liver-transplanted rats.
  
  
  METHODS
  Forty-eight male inbred Sprague-Dawley rats were randomly assigned into four groups: a sham-operation group and three groups with secondary biliary warm ischemia time of 0 min, 10 min and 20 min. A rat model of autologous liver transplantation under ether anesthesia was established, and six rats were killed in each group and blood samples and the median lobe of the liver were collected for assay at 6 h and 24 h after hepatic arterial reperfusion.
  
  
  RESULTS
  With prolongation of biliary warm ischemia time, the level of vascular endothelial growth factor-A was significantly decreased, and the value at 24 h was higher than that at 6 h after hepatic arterial reperfusion, but with no significant difference. The extended biliary SWIT led to a significant increase in bile duct epithelial cell apoptosis, and a decrease in the number of blood vessels, the bile duct surrounding the blood vessels and bile duct epithelial cell proliferation in the early postoperative portal area. Pathologic examinations showed that inflammation of the rat portal area was aggravated, and biliary epithelial cell injury was significantly worsened.
  
  
  CONCLUSIONS
  A prolonged biliary warm ischemia time results in aggravated injury of the bile duct and the surrounding vascular plexus in rat autologous orthotopic liver transplantation.
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Drum Tower Hospital, Medical School of Nanjing University, Nanjing 210008, Jiangsu Province, China.
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Zhu
authors.forename: Jun-Ping
authors.initials: JP
authors.lastname: Pan
authors.forename: Ya-Fu
authors.initials: YF
authors.lastname: Wu
authors.forename: Yi-Tao
authors.initials: YT
authors.lastname: Ding
mesh_terms: D000818:Animals; D017209:Apoptosis; D001653:Bile Ducts, Intrahepatic / Q000473:pathology / Q000601:surgery*; D001660:Biliary Tract Diseases / Q000097:blood / Q000209:etiology* / Q000473:pathology; D001808:Blood Vessels / Q000473:pathology; D049109:Cell Proliferation; D016031:Liver Transplantation / Q000009:adverse effects*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000097:blood; D052096:Warm Ischemia / Q000009:adverse effects*
chemical_list: D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat