title: Determination of microcystin-LR in water by a label-free aptamer based electrochemical impedance biosensor.
abstract: In this study, an electrochemical impedance biosensor for cyanobacterial toxin microcystin-LR (MC-LR) detection has been developed. MC-LR aptamers were immobilized on the gold electrode through Au-S interaction, in the presence of target (MC-LR); the binding of MC-LR and aptamers probe led to a complex formation change on the electrode surface and resulted in the impedance decreasing. The decrease rate had a linear relationship with logarithm of the MC-LR concentration in the range of 1.0 × 10(-7)-5.0 × 10(-11)mol/L, with a detection limit of 1.8 × 10(-11)mol/L. The sensor has good selectivity and stability, it has been applied to detect MC-LR in three kinds of real water samples with satisfying results.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350002, China. zylin@fzu.edu.cn
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Lin
authors.forename: Huiming
authors.initials: H
authors.lastname: Huang
authors.forename: Yixiang
authors.initials: Y
authors.lastname: Xu
authors.forename: Xiaoyao
authors.initials: X
authors.lastname: Gao
authors.forename: Bin
authors.initials: B
authors.lastname: Qiu
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D052157:Aptamers, Nucleotide / Q000378:metabolism*; D015374:Biosensing Techniques; D058266:Dielectric Spectroscopy; D017097:Electric Impedance; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D008387:Marine Toxins; D052998:Microcystins / Q000032:analysis*; D014867:Water / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D008387:Marine Toxins; D052998:Microcystins; D014867:Water; D006046:Gold; C057862:cyanoginosin LR