title: Healing, antioxidant and cytoprotective properties of Indigofera truxillensis in different models of gastric ulcer in rats.
abstract: The present study evaluated the antiulcerogenic activity and mechanisms of  the aqueous (AqF 100 mg/kg) and ethyl acetate (AcF 50 mg/kg) fractions from  Indigofera truxillensis leaves. This dose was selected to assess its activity on ulcer healing and its action on gastric acid and mucus secretion, prostaglandin production and antioxidant enzyme activity (superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and glutathione reductase (GSH-Rd)). Gastric ulcer was induced by absolute ethanol. Antisecretory action, mucus and prostaglandin production, healing and antioxidant enzyme activities were evaluated for both fractions. AqF and AcF significantly inhibited the gastric mucosal damage caused by ethanol. This effect was statistically significant at 100 and  50 mg/kg compared with the vehicle. Neither fraction interfered with gastric secretion. AcF increased the PGE(2) production, and both fractions increased mucus production. l-NAME did not alter the gastroprotection exerted by the fractions, but N-ethylmaleimide attenuated only AcF. In the ischemia/reperfusion model both fractions inhibited the mucosal damage. AcF increased SOD, GSH-Px and GSH-Rd activity, but AqF increased only SOD and GSH-Px. In the acetic acid-induced ulcer model AcF only accelerated ulcer healing. These results showed that Indigofera truxillensis acted as a gastroprotective agent, stimulating protective factors and antioxidants enzymes.
authors.affiliation: Department of Biological Sciences, Federal University of Goiás, 75704-020, Catalão, Goiás, Brazil. luiz_ferreiraa@yahoo.com.br.
authors.forename: Anderson
authors.initials: A
authors.lastname: Luiz-Ferreira
authors.forename: Maira
authors.initials: M
authors.lastname: Cola
authors.forename: Victor
authors.initials: V
authors.lastname: Barbastefano
authors.forename: Felipe Meira
authors.initials: FM
authors.lastname: de-Faria
authors.forename: Ana Beatriz A de
authors.initials: AB
authors.lastname: Almeida
authors.forename: Elisângela
authors.initials: E
authors.lastname: Farias-Silva
authors.forename: Tamara Regina
authors.initials: TR
authors.lastname: Calvo
authors.forename: Clélia A
authors.initials: CA
authors.lastname: Hiruma-Lima
authors.forename: Wagner
authors.initials: W
authors.lastname: Vilegas
authors.forename: Alba Regina M
authors.initials: AR
authors.lastname: Souza-Brito
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D004195:Disease Models, Animal; D000431:Ethanol / Q000009:adverse effects; D005750:Gastric Juice / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D029883:Indigofera / Q000737:chemistry*; D008297:Male; D055442:Metabolome; D055432:Metabolomics; D009569:Nitric Oxide / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D011453:Prostaglandins / Q000096:biosynthesis; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology; D064210:Secondary Metabolism; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D013438:Sulfhydryl Compounds / Q000378:metabolism / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000897:Anti-Ulcer Agents; D000975:Antioxidants; D010936:Plant Extracts; D011453:Prostaglandins; D020011:Protective Agents; D013438:Sulfhydryl Compounds; D009569:Nitric Oxide; D000431:Ethanol; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase