title: Apoptosis-inducing and antitumor activity of neolignans isolated from Magnolia officinalis in HeLa cancer cells.
abstract: Two neolignans, 4'-methoxymagndialdehyde (1) and magnaldehyde B (2), were isolated from the stem bark of Magnolia officinalis (Magnoliaceae), evaluated for apoptosis-inducing effects in human cervical epitheloid carcinoma HeLa cells. The apoptosis-inducing activity of compounds 1 and 2 were assessed by DNA content using flow cytometric analysis. In the immunoblotting analysis, the treatment with 1 and 2 resulted in the cleavage of procaspase-8 and -3 and poly(ADP-ribose)polymerase into active forms. In addition, in vivo, the administration of 2 to Lewis lung carcinoma-inoculated mice evidenced a significant inhibition of tumor growth (volume) with reduction of 28.7% at concentration of 20 mg/kg, as compared with the control mice. These findings suggest that 2 can inhibit the proliferation of tumor cells, and might be an anti-tumoric agent.
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, University of Hawaii at Hilo, 34 Rainbow Drive, Hilo, HI 96720, USA.
authors.forename: Ui Joung
authors.initials: UJ
authors.lastname: Youn
authors.forename: Nighat
authors.initials: N
authors.lastname: Fatima
authors.forename: Quan Cheng
authors.initials: QC
authors.lastname: Chen
authors.forename: Sungwook
authors.initials: S
authors.lastname: Chae
authors.forename: Tran Manh
authors.initials: TM
authors.lastname: Hung
authors.forename: Byung-Sun
authors.initials: BS
authors.lastname: Min
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D018827:Carcinoma, Lewis Lung; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D017705:Lignans / Q000494:pharmacology*; D031566:Magnolia / Q000737:chemistry*; D008297:Male; D051379:Mice; D024301:Plant Bark / Q000737:chemistry; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism
chemical_list: C524951:4'-methoxymagndialdehyde; D000972:Antineoplastic Agents, Phytogenic; D017705:Lignans; C587664:magnaldehyde B; D011065:Poly(ADP-ribose) Polymerases; C505407:CASP3 protein, human; C505530:CASP8 protein, human; D053148:Caspase 3; D053181:Caspase 8