title: Genetic characterization of a reptilian calicivirus (Cro1).
abstract: BACKGROUND
  Vesiviruses in the family Caliciviridae infect a broad range of animal hosts including mammals, birds, fish, amphibians and reptiles. The vesivirus Cro1 strains were isolated from diseased snakes in the San Diego zoo in 1978 and reported as the first caliciviruses found in reptiles. The goal of this study was to characterize the Cro1 strain 780032I that was isolated in cell culture from a rock rattlesnake (Crotalus lepidus) in the original outbreak.
  
  
  RESULTS
  We re-amplified the original virus stock in Vero cells, and determined its full-length genome sequence. The Cro1 genome is 8296 nucleotides (nt) in length and has a typical vesivirus organization, with three open reading frames (ORF), ORF1 (5643 nt), ORF2 (2121 nt), and ORF3 (348 nt) encoding a nonstructural polyprotein, the major capsid protein precursor, and a minor structural protein, respectively. Phylogenetic analysis of the full-length genome sequence revealed that the Cro1 virus clustered most closely with the VESV species of the genus Vesivirus, but was genetically distinct (82-83% identities with closest strains).
  
  
  CONCLUSIONS
  This is the first description of a full-length genome sequence from a reptile calicivirus (Cro1). The availability of the Cro1 genome sequence should facilitate investigation of the molecular mechanisms involved in Cro1 virus evolution and host range.
authors.affiliation: Caliciviruses Section/LID/NIAID/NIH, Bethesda, MD 20892, USA.
authors.forename: Carlos
authors.initials: C
authors.lastname: Sandoval-Jaime
authors.forename: Gabriel I
authors.initials: GI
authors.lastname: Parra
authors.forename: Alvin W
authors.initials: AW
authors.lastname: Smith
authors.forename: Kim Y
authors.initials: KY
authors.lastname: Green
authors.forename: Stanislav V
authors.initials: SV
authors.lastname: Sosnovtsev
mesh_terms: D000818:Animals; D000836:Animals, Zoo; D017250:Caliciviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D002140:California; D002522:Chlorocebus aethiops; D016000:Cluster Analysis; D017839:Crotalus / Q000821:virology*; D004196:Disease Outbreaks; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014709:Vero Cells; D029342:Vesivirus / Q000235:genetics* / Q000302:isolation & purification; D014776:Virus Cultivation
chemical_list: D012367:RNA, Viral