title: Evaluation of the cytotoxicity of different root canal sealers on L929 cell line by MTT assay.
abstract: The purpose of this study was to evaluate the cytotoxic effects of commercially available root canal sealers [Sealite Ultra (SU), Tubli-Seal (TS), Tubli-Seal EWT (TS-EWT), Pulp Canal Sealer (PCS), Pulp Canal Sealer EWT (PCS-EWT), Endomethasone N (En N), and Apexit Plus (AP)] on L929 cells by using MTT assay. After incubation with each sealer's extract at 37°C in a humidified air atmosphere containing 5% CO(2 )for 24 h, MTT (5 mg/mL) in saline was added into each well and further incubated at 37°C for 4 h. Formazan precipitate was dissolved in a buffer containing 23% sodium dodecyl sulfate and 50% N, N-dimethylformamide (pH 4.7). Optical densities of dissolved formazan were read using a microplate spectrophotometer. AP, TS, and TS-EWT showed no cytotoxicity at any dilution tested. Other sealers exhibited some degree of cytotoxicity at the 1/4 and 1/2 dilutions. PCS-EWT and SU exerted more potent cytotoxicity at 1/2 dilution than the other sealers.
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Hacettepe University, Ankara, Turkey. zeliha@hacettepe.edu.tr
authors.forename: Zeliha
authors.initials: Z
authors.lastname: Yilmaz
authors.forename: A Lale
authors.initials: AL
authors.lastname: Dogan
authors.forename: Ozgur
authors.initials: O
authors.lastname: Ozdemir
authors.forename: Ahmet
authors.initials: A
authors.lastname: Serper
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects*; D007739:L Cells; D051379:Mice; D012387:Root Canal Filling Materials / Q000633:toxicity*; D013778:Tetrazolium Salts; D013844:Thiazoles; D015035:Zinc Oxide-Eugenol Cement / Q000633:toxicity
chemical_list: D012387:Root Canal Filling Materials; D013778:Tetrazolium Salts; D013844:Thiazoles; D015035:Zinc Oxide-Eugenol Cement; C022616:thiazolyl blue