title: Anti-adhesive property of P-selectin glycoprotein ligand-1 (PSGL-1) due to steric hindrance effect.
abstract: P-selectin glycoprotein ligand-1 (PSGL-1) is an adhesive molecule that is known to be a ligand for P-selectin. An anti-adhesive property of PSGL-1 has not been previously reported. In this study, we show that PSGL-1 expression is anti-adhesive for adherent cells and we have elucidated the underlying mechanism. Overexpression of PSGL-1 induced cell rounding and floating in HEK293T cells. Similar phenomena were demonstrated in other adherent cell lines with overexpression of PSGL-1. PSGL-1 overexpression inhibits access of antibodies to cell surface molecules such as integrins, HLA and CD25. Cells transfected with PSGL-1 deletion mutants that lack a large part of the extracellular domain and chimeric construct expressing extracellular CD86 and intracellular PSGL-1 only showed rounded morphology, but there are no floating cells. These results indicated that PSGL-1 causes steric hindrance due to the extended structure of its extracellular domain that is highly O-glycosylated, but intracellular domain also has some effect on cell rounding. This study implies that PSGL-1 has Janus-faced functions, being both adhesive and anti-adhesive.
authors.affiliation: The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
authors.forename: Saori
authors.initials: S
authors.lastname: Umeki
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Suzuki
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ema
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shimojima
authors.forename: Yorihiro
authors.initials: Y
authors.lastname: Nishimura
authors.forename: Masaru
authors.initials: M
authors.lastname: Okuda
authors.forename: Takuya
authors.initials: T
authors.lastname: Mizuno
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D048430:Cell Shape; D005109:Extracellular Matrix / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D016023:Integrins / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000502:physiology*; D051379:Mice; D017434:Protein Structure, Tertiary
chemical_list: D016023:Integrins; D008562:Membrane Glycoproteins; C084882:P-selectin ligand protein