title: Diversity and adaptation of human respiratory syncytial virus genotypes circulating in two distinct communities: public hospital and day care center.
abstract: HRSV is one of the most important pathogens causing acute respiratory tract diseases as bronchiolitis and pneumonia among infants. HRSV was isolated from two distinct communities, a public day care center and a public hospital in São José do Rio Preto - SP, Brazil. We obtained partial sequences from G gene that were used on phylogenetic and selection pressure analysis. HRSV accounted for 29% of respiratory infections in hospitalized children and 7.7% in day care center children. On phylogenetic analysis of 60 HRSV strains, 48 (80%) clustered within or adjacent to the GA1 genotype; GA5, NA1, NA2, BA-IV and SAB1 were also observed. SJRP GA1 strains presented variations among deduced amino acids composition and lost the potential O-glycosilation site at amino acid position 295, nevertheless this resulted in an insertion of two potential O-glycosilation sites at positions 296 and 297. Furthermore, a potential O-glycosilation site insertion, at position 293, was only observed for hospital strains. Using SLAC and MEME methods, only amino acid 274 was identified to be under positive selection. This is the first report on HRSV circulation and genotypes classification derived from a day care center community in Brazil.
authors.affiliation: Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, SP. Departamento de Bioquímica e Imunologia–Av. dos Bandeirantes, 3900 Monte Alegre–Cep: 14049-900 Brazil. gugard@gmail.com
authors.forename: Luiz Gustavo Araujo
authors.initials: LG
authors.lastname: Gardinassi
authors.forename: Paulo Vitor Marques
authors.initials: PV
authors.lastname: Simas
authors.forename: Deriane Elias
authors.initials: DE
authors.lastname: Gomes
authors.forename: Caroline Measso
authors.initials: CM
authors.lastname: do Bonfim
authors.forename: Felipe Cavassan
authors.initials: FC
authors.lastname: Nogueira
authors.forename: Gustavo Rocha
authors.initials: GR
authors.lastname: Garcia
authors.forename: Claudia Márcia Aparecida
authors.initials: CM
authors.lastname: Carareto
authors.forename: Paula
authors.initials: P
authors.lastname: Rahal
authors.forename: Fátima Pereira
authors.initials: FP
authors.lastname: de Souza
mesh_terms: D000220:Adaptation, Biological; D000595:Amino Acid Sequence; D002648:Child; D002656:Child Day Care Centers; D002675:Child, Preschool; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006779:Hospitals, Public; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology; D018113:Respiratory Syncytial Virus, Human / Q000145:classification* / Q000235:genetics*; D012641:Selection, Genetic; D016415:Sequence Alignment; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins