title: Gibberellins in Dormant Embryos of Pyrus malus L. cv Golden delicious.
abstract: The aim of this work was to search for free gibberellins in dormant embryos of Pyrus malus L. cv Golden delicious using several extraction procedures. Quantitative estimations were made by biotest (lettuce hypocotyle test) and by GLC. Using an ethanolic extraction, only minute quantities of free gibberellins were detected. When extraction was carried out in the Tris buffer pH 7.2, the quantities of gibberellins detected were slightly higher. When the embryos were crushed in Tris buffer and treated with Triton X 100, very large amounts of gibberellins were detected, especially GA1 and GA4. These results indicate that gibberellins are present in dormant embryos before chilling treatment. The form in which the gibberellins exist and the origin of GA4 are discussed.
authors.affiliation: Laboratoire de Physiologie végétale, Université de Nice, 28 Avenue Valrose, 06 034 Nice Cédex, France.
authors.forename: J
authors.initials: J
authors.lastname: Bianco
authors.forename: S
authors.initials: S
authors.lastname: Lasséchère
authors.forename: C
authors.initials: C
authors.lastname: Bulard