title: FATP1 silence inhibits the differentiation and induces the apoptosis in chicken preadipocytes.
abstract: FATP1 plays an important role in the trafficking of free fatty acids in adipocytes, however, its precise function and relationship with other fatty acid transporters all remain poorly understood. In this study, FATP1 gene silencing was induced by transfecting siRNA of target sequence into chicken preadipocytes, then the expression of FABP was found down-regulated while the expression of FAT was raised. In addition, differential inhibition of the cells was observed and the expressions of PPARγ and C/EBPα were found down-regulated. Moreover, the silencing also induced the down-regulation of FAS and inhibited the adipogenesis in adipocytes. Of specific interest here was that FATP1 silencing significantly improved the expressions and activities of cell apoptotic factors Caspases 3 and BCL2 associated X protein (Bax). Consequently, FATP1 deficiency prevented the differentiation while induced apoptosis in chicken preadipocytes.
authors.affiliation: College of Animal Science & Technology, Northwest A&F University, Yangling 712100, China.
authors.forename: Renli
authors.initials: R
authors.lastname: Qi
authors.forename: Min
authors.initials: M
authors.lastname: Feng
authors.forename: Xiao
authors.initials: X
authors.lastname: Tan
authors.forename: Lu
authors.initials: L
authors.lastname: Gan
authors.forename: Guoyong
authors.initials: G
authors.lastname: Yan
authors.forename: Chao
authors.initials: C
authors.lastname: Sun
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000235:genetics; D053148:Caspase 3 / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics*; D002645:Chickens / Q000235:genetics / Q000254:growth & development; D050612:Fatty Acid Transport Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D020868:Gene Silencing; D047495:PPAR gamma / Q000235:genetics; D019014:fas Receptor / Q000235:genetics
chemical_list: D022782:CCAAT-Enhancer-Binding Protein-beta; D050612:Fatty Acid Transport Proteins; D047495:PPAR gamma; D019014:fas Receptor; D053148:Caspase 3