title: Network motifs in the transcriptional regulation network of cervical carcinoma cells respond to EGF.
abstract: OBJECTIVE
  Cervical carcinoma is the second most prevalent and the fifth most deadly malignancy seen in women worldwide. Dysregulated activation of EGF ErbB system has been implicated in diverse types of human cancer; however, it is elusive how it is regulated in human cervical cancer cells. We herein aimed to explore the mechanisms of cervical carcinoma response to epidermal growth factor (EGF), with a view of the pathways activated by EGF.
  
  
  METHODS
  Using the GSE6783 affymetrix microarray data accessible from gene expression omnibus database, we first identified the differentially expressed genes between EGF-stimulated and -unstimulated samples. Then we constructed a regulation network and identified the network motifs. We also performed biological process and pathway enrichment analyses to functionally classify the genes in the regulation network.
  
  
  RESULTS
  A total of 11 network motifs were identified in the regulation network. EGF treatment could increase the risk of cancer via dysregulation of cancer-related pathways and immune response pathways.
  
  
  CONCLUSIONS
  Network motif analysis is useful in mining the useful information underlying the network. We hope our work could serve as a basis for further experimentation.
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai First People Hospital, Shanghai Jiaotong University, No.100 Haining Road, Hongkou District 200080, Shanghai, China. wusufangsf@hotmail.com
authors.forename: Su Fang
authors.initials: SF
authors.lastname: Wu
authors.forename: Wen Yan
authors.initials: WY
authors.lastname: Qian
authors.forename: Jia Wen
authors.initials: JW
authors.lastname: Zhang
authors.forename: Yong Bin
authors.initials: YB
authors.lastname: Yang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Dong
authors.forename: Zhen Bo
authors.initials: ZB
authors.lastname: Zhang
authors.forename: Ya Ping
authors.initials: YP
authors.lastname: Zhu
authors.forename: You Ji
authors.initials: YJ
authors.lastname: Feng
mesh_terms: D002277:Carcinoma / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D006367:HeLa Cells; D006801:Humans; D002583:Uterine Cervical Neoplasms / Q000378:metabolism*
chemical_list: D004815:Epidermal Growth Factor