title: Development of a semi-defined medium supporting production of platensimycin and platencin by Streptomyces platensis.
abstract: Platensimycin and platencin are compounds that were discovered at Merck Research Laboratories and have shown promising antibacterial activity. They are both produced in fermentation by the actinomycete Streptomyces platensis. Merck reported a crude, insoluble production medium to produce the antibiotics. To test the possible effects of different primary metabolites and inorganic compounds on the production of these antibiotics, a chemically-defined medium is needed. The effects that these compounds have on production could provide information about the precursors and biosynthetic pathway of the antibiotics. We have tested and developed a number of media with varying degrees of chemical definition and solubility using the Merck medium as our starting point. Our latest production medium, PM5, is soluble and semi-defined. It yields suitable production of the compounds, as shown by agar diffusion assays, bioautography and HPLC. The antibiotics were located in the extracellular broths and not in the mycelia.
authors.affiliation: Charles A Dana Research Institute for Scientists Emerti, Drew University, Madison, NJ, USA.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Aluotto
authors.forename: Heather
authors.initials: H
authors.lastname: Tynan
authors.forename: Christian
authors.initials: C
authors.lastname: Maggio
authors.forename: Maria
authors.initials: M
authors.lastname: Falzone
authors.forename: Anisha
authors.initials: A
authors.lastname: Mukherjee
authors.forename: Vincent
authors.initials: V
authors.lastname: Gullo
authors.forename: Arnold L
authors.initials: AL
authors.lastname: Demain
mesh_terms: D000218:Adamantane / Q000138:chemical synthesis* / Q000494:pharmacology; D062365:Aminobenzoates / Q000138:chemical synthesis* / Q000494:pharmacology; D000627:Aminophenols / Q000138:chemical synthesis* / Q000494:pharmacology; D000813:Anilides / Q000138:chemical synthesis* / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000494:pharmacology; D003470:Culture Media; D005285:Fermentation; D011083:Polycyclic Compounds / Q000138:chemical synthesis* / Q000494:pharmacology; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D062365:Aminobenzoates; D000627:Aminophenols; D000813:Anilides; D000900:Anti-Bacterial Agents; D003470:Culture Media; D011083:Polycyclic Compounds; D000218:Adamantane; C510909:platensimycin; C520340:platencin