title: Immunosuppressive effect of regulatory T lymphocytes in lung cancer, with special reference to their effects on the induction of autologous tumor-specific cytotoxic T lymphocytes.
abstract: It is not easy to induce cytotoxic T lymphocytes (CTLs) against cancer in in vitro culture. Regulatory T cells (Tregs) are considered to play a pivotal role in tumor immune escape. In this study, we analyzed the distribution of Tregs among tumor-infiltrating lymphocytes (TILs), regional lymph node lymphocytes (RLNLs) and peripheral blood lymphocytes (PBLs) in patients with lung cancer, and analyzed the effect of Tregs on the induction of CTLs in vitro. A total of 84 patients with non-small cell lung cancer underwent surgery between January 2003 and December 2004. The TILs, RLNLs and PBLs from these patients were subjected to a comparison analysis. The proportion of CD4(+)CD25(+)Foxp3(+) cells in these lymphocytes was determined by flow cytometry. The effects of Tregs on the induction of CTLs was analyzed by the depletion of Tregs in mixed lymphocyte-tumor cell culture (MLTC). The average proportions of Tregs in the TILs, RLNLs and PBLs were 10.4±9.5, 4.4±2.4 and 2.8±2.1%, respectively. The proportion of Tregs in the RLNLs was significantly higher than that in the PBLs (P<0.001); furthermore, TILs contained a larger number of Tregs than RLNLs (P=0.034). These Tregs substantially suppressed the induction of CTLs against autologous tumor cells. The depletion of Tregs in the MLTC resulted in the successful induction of CTLs. Tregs were found at a higher frequency in the TILs and RLNLs than in the PBLs in lung cancer patients. Since Tregs inhibited the induction of CTLs, the depletion of Tregs may represent a new therapeutic strategy for lung cancer patients.
authors.affiliation: Second Department of Surgery, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Yahatanishi 807-8555,  Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Shigematsu
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hanagiri
authors.forename: Hironobu
authors.initials: H
authors.lastname: Shiota
authors.forename: Koji
authors.initials: K
authors.lastname: Kuroda
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Baba
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Ichiki
authors.forename: Manabu
authors.initials: M
authors.lastname: Yasuda
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Uramoto
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Takenoyama
authors.forename: Kosei
authors.initials: K
authors.lastname: Yasumoto
authors.forename: Fumihiro
authors.initials: F
authors.lastname: Tanaka