title: miR-206 regulates the growth of the teleost tilapia (Oreochromis niloticus) through the modulation of IGF-1 gene expression.
abstract: MicroRNAs (miRNAs) are ~22-nucleotide noncoding RNAs that play a crucial role in regulating muscle development. Our previous study shows that miR-206 is specifically expressed in tilapia skeletal muscle, and exhibits a dynamic expression pattern at different developmental stages. Here, we reveal that miR-206 emerges as a crucial regulator of tilapia growth. miR-206 loss of function leads to the acceleration of tilapia growth. IGF-1 is identified as the target gene of miR-206. miR-206 directly changes IGF-1 expression by targeting its 3' UTR, and inhibition of miR-206 substantially increases the IGF-1 mRNA level in vivo. Thus, miR-206 could be developed as a molecular marker to assist fish breeding.
authors.affiliation: Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Yan
authors.forename: Chang-Dong
authors.initials: CD
authors.lastname: Zhu
authors.forename: Jin-Tao
authors.initials: JT
authors.lastname: Guo
authors.forename: Li-Hui
authors.initials: LH
authors.lastname: Zhao
authors.forename: Jin-Liang
authors.initials: JL
authors.lastname: Zhao
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D023681:Cichlids / Q000254:growth & development* / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D020868:Gene Silencing; D057809:HEK293 Cells; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D008156:Luciferases; D035683:MicroRNAs / Q000378:metabolism* / Q000494:pharmacology; D018482:Muscle, Skeletal / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D017931:DNA Primers; D035683:MicroRNAs; D007334:Insulin-Like Growth Factor I; D008156:Luciferases