title: High-resolution monolithic columns--a new tool for effective and quick separation.
abstract: This work describes a comparison of three types of commercial high-performance liquid chromatography silica monolithic columns with different inner diameters and generations of monolithic sorbent: a "classic" monolithic column, the first generation (Onyx™ monolithic C(18), 100 mm × 4.6 mm, Phenomenex); a "narrow" monolithic column for fast separation at lower flow rates (Chromolith® Performance RP-18e, 100 mm × 3 mm, Merck); and a recently introduced "high-resolution" monolithic column, the next generation (Chromolith® HighResolution RP-18e, 100 mm × 4.6 mm, Merck). Separation efficiency (number of theoretical plates, height equivalent to a theoretical plate and van Deemter curves), working pressure, the symmetry factor and resolution were critical aspects of the comparison in the case of the separation of ascorbic acid, paracetamol and caffeine. The separations were performed under isocratic conditions with a mobile phase consisting of 10:90 (v/v) acetonitrile-phosphoric acid (pH 2.80). Detailed comparison of the newest-generation monolithic column (Chromolith® HighResolution) with the previously introduced monolithic sorbents was performed and proved the advantages of the Chromolith® HighResolution column.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Sklenářová
authors.forename: Petr
authors.initials: P
authors.lastname: Chocholouš
authors.forename: Petra
authors.initials: P
authors.lastname: Koblová
authors.forename: Lukáš
authors.initials: L
authors.lastname: Zahálka
authors.forename: Dalibor
authors.initials: D
authors.lastname: Šatínský
authors.forename: Ludmila
authors.initials: L
authors.lastname: Matysová
authors.forename: Petr
authors.initials: P
authors.lastname: Solich
mesh_terms: D000082:Acetaminophen / Q000737:chemistry / Q000302:isolation & purification*; D000327:Adsorption; D001205:Ascorbic Acid / Q000737:chemistry / Q000302:isolation & purification*; D002110:Caffeine / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*
chemical_list: D000082:Acetaminophen; D002110:Caffeine; D001205:Ascorbic Acid