title: Putative and unique gene sequence utilization for the design of species specific probes as modeled by Lactobacillus plantarum.
abstract: The concept of utilizing putative and unique gene sequences for the design of species specific probes was tested. The abundance profile of assigned functions within the Lactobacillus plantarum genome was used for the identification of the putative and unique gene sequence, csh. The targeted gene (csh) was used as the template for PCR amplification and construction of a non-radioactive DIG labeled probe. The csh derived probe aided in the preliminary and rapid identification of L. plantarum from mixed cultures by colony hybridization. The method described here for the rapid identification of L. plantarum can also be applied for the rapid detection of other bacteria if a unique gene sequence can be identified from its complete genome sequence.
authors.affiliation: USDA-ARS, SAA Food Science Research Unit, North Carolina State University, 322 Schaub Hall, Box 7624, Raleigh, NC 27695-7624, USA. Ilenys.Perez-Diaz@ars.usda.gov
authors.forename: Ilenys M
authors.initials: IM
authors.lastname: Pérez-Díaz
mesh_terms: D005798:Genes, Bacterial; D048191:Lactiplantibacillus plantarum / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D015341:Nucleic Acid Probes; D016133:Polymerase Chain Reaction; D013045:Species Specificity
chemical_list: D015341:Nucleic Acid Probes