title: Rapid fingerprint analysis of Radix Scutellariae by UFLC-DAD.
abstract: To better control the quality of Radix Scutellariae (RS), a fast fingerprint method based on ultra-fast liquid chromatography coupled with diode array detection (UFLC-DAD) was developed. In this study, the analysis time of ∼85 min when using conventional high-performance liquid chromatography was shortened to ∼40 min on UFLC. The UFLC method was validated in terms of stability [<5.80% relative standard deviation (RSD)], precision (<3.48% RSD) and reproducibility (<3.56% RSD). Finally, eight batches of RS were analyzed by UFLC-DAD, and their similarities were systematically processed with professional analytical software, Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (Version 2004A), which was recommended by State Food and Drug Administration of China. The developed UFLC method is efficient, reproducible, stable, precise, and can be used as a more efficient approach for the quality monitoring and assessment of RS.
authors.affiliation: 1Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Huanggang Normal University, Huangzhou 438000, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Long
authors.forename: Jingjing
authors.initials: J
authors.lastname: Yu
authors.forename: Yingying
authors.initials: Y
authors.lastname: Huang
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Shi
authors.forename: Lanying
authors.initials: L
authors.lastname: Xu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D018517:Plant Roots / Q000737:chemistry*; D015203:Reproducibility of Results; D031364:Scutellaria baicalensis / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal