title: Crystallization and preliminary X-ray crystallographic analysis of quinolinate phosphoribosyltransferase from porcine kidney in complex with nicotinate mononucleotide.
abstract: Quinolinate phosphoribosyltransferase (QAPRTase) is a key enzyme in NAD biosynthesis; it catalyzes the formation of nicotinate mononucleotide (NAMN) from quinolinate and 5-phosphoribosyl-1-pyrophosphate. In order to elucidate the mechanism of NAMN biosynthesis, crystals of Sus scrofa QAPRTase (Ss-QAPRTase) purified from porcine kidney in complex with NAMN were obtained and diffraction data were collected and processed to 2.1 Å resolution. The Ss-QAPRTase-NAMN cocrystals belonged to space group P321, with unit-cell parameters a=119.1, b=119.1, c=93.7 Å, γ=120.0°. The Matthews coefficient and the solvent content were estimated as 3.10 Å3 Da(-1) and 60.3%, respectively, assuming the presence of two molecules in the asymmetric unit.
authors.affiliation: School of Life Sciences, Cell Dynamics Research Center, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea.
authors.forename: Hyung-Seop
authors.initials: HS
authors.lastname: Youn
authors.forename: Mun-Kyoung
authors.initials: MK
authors.lastname: Kim
authors.forename: Gil Bu
authors.initials: GB
authors.lastname: Kang
authors.forename: Tae Gyun
authors.initials: TG
authors.lastname: Kim
authors.forename: Jun Yop
authors.initials: JY
authors.lastname: An
authors.forename: Jung-Gyu
authors.initials: JG
authors.lastname: Lee
authors.forename: Kyoung Ryoung
authors.initials: KR
authors.lastname: Park
authors.forename: Youngjin
authors.initials: Y
authors.lastname: Lee
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Fukuoka
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Eom
mesh_terms: D000818:Animals; D018360:Crystallography, X-Ray; D007668:Kidney / Q000201:enzymology*; D008958:Models, Molecular; D009537:Nicotinamide Mononucleotide / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D010430:Pentosyltransferases / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D013552:Swine / Q000378:metabolism; D000098553:Nicotinate-Nucleotide Diphosphorylase (Carboxylating)
chemical_list: D009537:Nicotinamide Mononucleotide; C002953:nicotinate mononucleotide; D010430:Pentosyltransferases; D000098553:Nicotinate-Nucleotide Diphosphorylase (Carboxylating)