title: Fluorescence spectroscopy of iron-deficient plants.
abstract: Iron deficient cucumber (Cucumis sativus L.) plants and such differently supplied with iron were studied by low-temperature fluorescence emission and excitation spectroscopy. Iron deficiency caused a relative decrease and a blue-shift of the 733 nm emission and a decrease or disappearance of the 700 and 707-710 nm excitation bands which are considered to belong to chlorophyll forms of PSI. The process of iron uptake was observed after readdition of iron to previously iron-stressed plants. Iron-deficient plants (with green cotyledon) were treated with the chelator EDTA which treatment resulted in a small increase of the chlorophyll content but the fluorescence spectra of these plants differed from those of plants supplied with iron: they had blue-shifted maxima. The data presented show that fluorescence spectroscopy is a very sensitive and suitable method for studying iron deficiency, observing - indirectly - iron uptake and its utilization in plants.
authors.affiliation: Department of Plant Physiology, Eötvös Loránd University Budapest, Muzeum krt 4/a, 1088 Hungary.
authors.forename: B
authors.initials: B
authors.lastname: Böddi
authors.forename: E
authors.initials: E
authors.lastname: Cseh
authors.forename: F
authors.initials: F
authors.lastname: Láng