title: Sugar beet M14 glyoxalase I gene can enhance plant tolerance to abiotic stresses.
abstract: Glyoxalase I is the first enzyme of the glyoxalase system that can detoxify methylglyoxal, a cytotoxic compound increased rapidly under stress conditions. Here we report cloning and characterization of a glyoxalase I from sugar beet M14 line (an interspecific hybrid between a wild species Beta corolliflora Zoss and a cultivated species B. vulgaris L). The full-length gene BvM14-glyoxalase I has 1,449 bp in length with an open reading frame of 1,065 bp encoding 354 amino acids. Sequence analysis shows the conserved glyoxalase I domains, metal and glutathione binding sites and secondary structure (α-helixes and β-sheets). The BvM14-glyoxalase I gene was ubiquitously expressed in different tissues of sugar beet M14 line and up-regulated in response to salt, mannitol and oxidative stresses. Heterologous expression of BvM14-glyoxalase I could increase E. coli tolerance to methylglyoxal. Transgenic tobacco plants constitutively expressing BvM14-glyoxalase I were generated. Both leaf discs and seedlings showed significant tolerance to methylglyoxal, salt, mannitol and H2O2. These results suggest an important role of BvM14-glyoxalase I in cellular detoxification and tolerance to abiotic stresses.
authors.affiliation: College of Life Sciences, Heilongjiang University, Harbin, China. wuchuan8859@126.com
authors.forename: Chuan
authors.initials: C
authors.lastname: Wu
authors.forename: Chunquan
authors.initials: C
authors.lastname: Ma
authors.forename: Yu
authors.initials: Y
authors.lastname: Pan
authors.forename: Shilong
authors.initials: S
authors.lastname: Gong
authors.forename: Chenxi
authors.initials: C
authors.lastname: Zhao
authors.forename: Sixue
authors.initials: S
authors.lastname: Chen
authors.forename: Haiying
authors.initials: H
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D027461:Beta vulgaris / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism; D007791:Lactoylglutathione Lyase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008353:Mannitol / Q000378:metabolism; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011765:Pyruvaldehyde / Q000378:metabolism*; D016415:Sequence Alignment; D012965:Sodium Chloride / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D008353:Mannitol; D012965:Sodium Chloride; D011765:Pyruvaldehyde; D006861:Hydrogen Peroxide; D007791:Lactoylglutathione Lyase