title: Purification, crystallization and preliminary X-ray analysis of two hydrogen sulfide-producing enzymes from Fusobacterium nucleatum.
abstract: Hydrogen sulfide produced by oral bacteria is responsible for oral malodour. Two homologous hydrogen sulfide-producing enzymes, Fn1220 and Cdl, from Fusobacterium nucleatum (which actively produces hydrogen sulfide) were overproduced, purified and crystallized. X-ray diffraction data were collected from the crystals using a synchrotron-radiation source. The Fn1220 crystal belonged to tetragonal space group P4(1)2(1)2 or P4(3)2(1)2 (unit-cell parameters a=b=116.8, c=99.2 Å) and the Cdl crystal belonged to monoclinic space group P2(1) (unit-cell parameters a=84.9, b=70.9, c=87.6 Å, β=90.3°).
authors.affiliation: Department of Structural Biology, School of Pharmacy, Iwate Medical University, Yahaba, Iwate 028-3694, Japan. ykezuka@iwate-med.ac.jp
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Kezuka
authors.forename: Naoto
authors.initials: N
authors.lastname: Abe
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Takamasa
authors.initials: T
authors.lastname: Nonaka
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D016967:Fusobacterium nucleatum / Q000201:enzymology* / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D006862:Hydrogen Sulfide