title: Endometrial epithelial cells are potent producers of tracheal antimicrobial peptide and serum amyloid A3 gene expression in response to E. coli stimulation.
abstract: Endometrial epithelial cells play a critical role in mediating inflammatory mechanisms key to bacterial clearance and tissue re-modelling postpartum. This study characterised innate immune gene expression by bovine endometrial epithelial cells from three animals in response to Escherichia coli, a common cause of bovine uterine disease. Expression of key innate immune genes, encoding Toll-like receptor 4 (TLR4), the transcription factor NFkB1, the chemokine interleukin 8 (IL8), inflammatory cytokines (interleukins IL1β, IL6; tumour necrosis factor, TNF), β-defensins (lingual antimicrobial peptides LAP, tracheal antimicrobial peptide TAP) and acute phase proteins (haptoglobin, HP; serum amyloid A, SAA3) was examined in endometrial epithelial cells stimulated with E. coli for 6 and 24h using qRT-PCR. Expression of all genes was increased significantly (P<0.05) 6h post-stimulation. Expression of IL1b, TNF and SAA3 genes was increased by 121-, 357- and 721-fold, respectively (P<0.05). Twenty four hours post-stimulation, IL1b, IL6, IL8, TNF and LAP gene expression was decreased compared to 6h, whereas TAP and SAA3 expression was further increased to 209- and 3452-fold (P<0.05). E. coli driven expression of immune effector genes demonstrates potent immune, antimicrobial and regulatory capacity of endometrial epithelial cells to respond to this pathogen.
authors.affiliation: Comparative Immunology Group, School of Biochemistry and Immunology, Trinity College, Dublin 2, Ireland.
authors.forename: Aspinas
authors.initials: A
authors.lastname: Chapwanya
authors.forename: Kieran G
authors.initials: KG
authors.lastname: Meade
authors.forename: Michael L
authors.initials: ML
authors.lastname: Doherty
authors.forename: John J
authors.initials: JJ
authors.lastname: Callanan
authors.forename: Cliona
authors.initials: C
authors.lastname: O'Farrelly
mesh_terms: D000209:Acute-Phase Proteins / Q000235:genetics; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics*; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics / Q000276:immunology; D016207:Cytokines / Q000235:genetics; D004716:Endometritis / Q000235:genetics / Q000276:immunology / Q000662:veterinary; D004717:Endometrium / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D004847:Epithelial Cells / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D004926:Escherichia coli / Q000276:immunology* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary; D005260:Female; D015870:Gene Expression; D007113:Immunity, Innate / Q000235:genetics; D000685:Serum Amyloid A Protein / Q000235:genetics*
chemical_list: D000209:Acute-Phase Proteins; D023181:Antimicrobial Cationic Peptides; D016207:Cytokines; D000685:Serum Amyloid A Protein; C068629:tracheal antimicrobial peptide, bovine