title: Structure of ribose 5-phosphate isomerase from the probiotic bacterium Lactobacillus salivarius UCC118.
abstract: The structure of ribose 5-phosphate isomerase from the probiotic bacterium Lactobacillus salivarius UCC188 has been determined at 1.72 Å resolution. The structure was solved by molecular replacement, which identified the functional homodimer in the asymmetric unit. Despite only showing 57% sequence identity to its closest homologue, the structure adopted the typical α and β D-ribose 5-phosphate isomerase fold. Comparison to other related structures revealed high homology in the active site, allowing a model of the substrate-bound protein to be proposed. The determination of the structure was expedited by the use of in situ crystallization-plate screening on beamline I04-1 at Diamond Light Source to identify well diffracting protein crystals prior to routine cryocrystallography.
authors.affiliation: Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0DE, England.
authors.forename: Carina M C
authors.initials: CM
authors.lastname: Lobley
authors.forename: Pierre
authors.initials: P
authors.lastname: Aller
authors.forename: Alice
authors.initials: A
authors.lastname: Douangamath
authors.forename: Yamini
authors.initials: Y
authors.lastname: Reddivari
authors.forename: Mario
authors.initials: M
authors.lastname: Bumann
authors.forename: Louise E
authors.initials: LE
authors.lastname: Bird
authors.forename: Joanne E
authors.initials: JE
authors.lastname: Nettleship
authors.forename: Jose
authors.initials: J
authors.lastname: Brandao-Neto
authors.forename: Raymond J
authors.initials: RJ
authors.lastname: Owens
authors.forename: Paul W
authors.initials: PW
authors.lastname: O'Toole
authors.forename: Martin A
authors.initials: MA
authors.lastname: Walsh
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000737:chemistry* / Q000378:metabolism; D000595:Amino Acid Sequence; D001665:Binding Sites; D018360:Crystallography, X-Ray; D019281:Dimerization; D007778:Lactobacillus / Q000201:enzymology*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017510:Protein Folding; D016415:Sequence Alignment
chemical_list: D019747:Aldose-Ketose Isomerases; C022548:ribosephosphate isomerase