title: Effect of β-cyclodextrin complexation on solubility and enzymatic conversion of naringin.
abstract: In the present paper, the effect of beta-cyclodextrin (beta-CD) inclusion complexation on the solubility and enzymatic hydrolysis of naringin was investigated. The inclusion complex of naringin/beta-CD at the molar ratio of 1:1 was obtained by the dropping method and was characterized by differential scanning calorimetry. The solubility of naringin complexes in water at 37 ± 0.1 °C was 15 times greater than that of free naringin. Snailase-involved hydrolysis conditions were tested for the bioconversion of naringin into naringenin using the univariate experimental design. Naringin can be transformed into naringenin by snailase-involved hydrolysis. The optimum conditions for enzymatic hydrolysis were determined as follows: pH 5.0, temperature 37 °C, ratio of snailase/substrate 0.8, substrate concentration 20 mg·mL(-1), and reaction time 12 h. Under the optimum conditions, the transforming rate of naringenin from naringin for inclusion complexes and free naringin was 98.7% and 56.2% respectively, suggesting that beta-CD complexation can improve the aqueous solubility and consequently the enzymatic hydrolysis rate of naringin.
authors.affiliation: Key Laboratory of New Drug Delivery System of Chinese Meteria Medica,  Jiangsu Provincial Academy of Chinese Medicine, 100 Shizi Road, Nanjing 210028,  Jiangsu, China. xiaobinjia_nj@126.com.
authors.forename: Li
authors.initials: L
authors.lastname: Cui
authors.forename: Zhen-Hai
authors.initials: ZH
authors.lastname: Zhang
authors.forename: E
authors.initials: E
authors.lastname: Sun
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Jia
mesh_terms: D002152:Calorimetry, Differential Scanning; D044950:Flavanones / Q000737:chemistry*; D006868:Hydrolysis; D007700:Kinetics; D015203:Reproducibility of Results; D012995:Solubility; D013696:Temperature; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D044950:Flavanones; D047392:beta-Cyclodextrins; C005274:naringin