title: Synthesis, photophysical properties, and application of o- and p-amino green fluorescence protein synthetic chromophores.
abstract: The o- and p-amino green-fluorescence-protein synthetic chromophores (GFPSCs) were synthesized from the corresponding o- and p-nitro protecting group. Among the four protecting groups of the o-amino group, the o-nitro protecting group is the only choice to synthesize the o-amino GFPSCs. The first singlet excited states of o- and p-amino GFPSCs carry significant charge-transfer character through the mechanism of photoinduced charge transfer (PCT). The o-amino GFPSCs can serve as wavelength-ratiometric fluorescence sensors that selectively recognize Cr(3+) in aqueous medium through a PCT mechanism.
authors.affiliation: Department of Chemistry, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Yi-Hui
authors.initials: YH
authors.lastname: Chen
authors.forename: Wei-Jen
authors.initials: WJ
authors.lastname: Lo
authors.forename: Kuangsen
authors.initials: K
authors.lastname: Sung
mesh_terms: D002857:Chromium / Q000737:chemistry*; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000138:chemical synthesis* / Q000737:chemistry; D010777:Photochemistry; D012996:Solutions / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D012996:Solutions; D002857:Chromium; D049452:Green Fluorescent Proteins