title: Comparison of Celsior and Perfadex lung preservation solutions in rat lungs subjected to 6 and 12 hours of ischemia using an ex-vivo lung perfusion system.
abstract: OBJECTIVE
  This study evaluated the performance of lungs that were preserved with different solutions (Celsior, Perfadex or saline) in an ex vivo rat lung perfusion system.
  
  
  METHODS
  Sixty Wistar rats were anesthetized, anticoagulated and randomized into three groups (n = 20). The rats were subjected to antegrade perfusion via the pulmonary artery with Perfadex, Celsior, or saline, followed by 6 or 12 hours of ischemia (4ºC, n = 10 in each group). Respiratory mechanics, gas exchange and hemodynamics were measured at 10-minute intervals during the reperfusion of heart-lung blocks in an ex vivo system (IL2-Isolated Perfused Rat or Guinea Pig Lung System, Harvard Apparatus, Holliston, Massachusetts, USA; Hugo Sachs Elektronik, Germany) for 60 minutes. The lungs were prepared for histopathology and evaluated for edema following reperfusion. Group comparisons were performed using ANOVA and the Kruskal-Wallis test with a 5% level of significance.
  
  
  RESULTS
  Gas exchange was not significantly different between lungs perfused with either Perfadex or Celsior at the same ischemic times, but it was very low in lungs that were preserved with saline. Airway resistance was greater in the lungs that were preserved for 12 hours. Celsior lungs that were preserved for 6 and 12 hours exhibited lower airway resistance (p = 0.01) compared to Perfadex lungs. Pulmonary artery pressure was not different between the groups, and no significant differences in histopathology and apoptosis were observed between the groups.
  
  
  CONCLUSIONS
  Lungs that were preserved with Celsior or Perfadex exhibited similar gas exchange and histopathological findings. Airway resistance was slightly lower in the Celsior-preserved lungs compared with the Perfadex-preserved lungs.
authors.affiliation: Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Heart Institute (InCor), São Paulo/SP, Brazil.
authors.forename: Arteiro Queiroz
authors.initials: AQ
authors.lastname: Menezes
authors.forename: Paulo Manuel
authors.initials: PM
authors.lastname: Pêgo-Fernandes
authors.forename: Paulo Francisco Guerreiro
authors.initials: PF
authors.lastname: Cardoso
authors.forename: Karina Andrighetti de Oliveira
authors.initials: KA
authors.lastname: Braga
authors.forename: Natalia Aparecida
authors.initials: NA
authors.lastname: Nepomuceno
authors.forename: Rogerio
authors.initials: R
authors.lastname: Pazetti
authors.forename: Aristides Tadeu
authors.initials: AT
authors.lastname: Correia
authors.forename: Mauro
authors.initials: M
authors.lastname: Canzian
authors.forename: Jacqueline Klarosk
authors.initials: JK
authors.lastname: Santim
authors.forename: Fabio Biscegli
authors.initials: FB
authors.lastname: Jatene
mesh_terms: D000818:Animals; D002951:Citrates; D004187:Disaccharides; D004573:Electrolytes; D005971:Glutamates; D005978:Glutathione; D006639:Histidine; D007511:Ischemia; D008168:Lung / Q000098:blood supply / Q000473:pathology; D016040:Lung Transplantation; D008297:Male; D008353:Mannitol; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D010477:Perfusion / Q000379:methods; D011659:Pulmonary Gas Exchange / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012965:Sodium Chloride; D013997:Time Factors
chemical_list: C088453:Celsior; D002951:Citrates; D004187:Disaccharides; D004573:Electrolytes; D005971:Glutamates; D019188:Organ Preservation Solutions; C070005:Perfadex; D008353:Mannitol; D012965:Sodium Chloride; D006639:Histidine; D005978:Glutathione