title: Pre-treatment with curcumin modulates acetylcholinesterase activity and proinflammatory cytokines in rats infected with Trypanosoma evansi.
abstract: The potent activity against Trypanosomes and health beneficial effects of curcumin (Cur) has been demonstrated in various experimental models. In this study, we evaluated the in vivo effect of Cur as trypanocide and as potential anti-inflammatory agent, through the evaluation of immunomodulatory mechanisms in rats infected with Trypanosoma evansi. Daily oral Cur was administered at doses of 0, 20 or 60mg/kg as preventive treatment (30 and 15days pre infection) and as treatment (post infection). The treatment of the groups continued until the day of euthanasia. Fifteen days after inoculation, parasitemia, plasma proinflammatory cytokines (IFN-γ, TNF-α, IL-1, IL-6), anti-inflammatory cytokines (IL-10) and blood acetylcholinesterase activity (AChE) were analyzed. Pretreatment with Cur reduced parasitemia and lethality. Cur inhibited AChE activity and improved immunological response by cytokines proinflammatory, fundamental during T. evansi infection. We found that Cur is not so important as an antitrypanosomal activity but as immunomodulator agent. These findings reveal that the preventive use of Cur stimulates anti-inflammatory mechanisms, reducing an excessive inflammatory response.
authors.affiliation: Department of Chemistry, Universidade Federal de Santa Maria, Brazil. patiwol@hotmail.com
authors.forename: Patrícia
authors.initials: P
authors.lastname: Wolkmer
authors.forename: Cássia B da
authors.initials: CB
authors.lastname: Silva
authors.forename: Francine C
authors.initials: FC
authors.lastname: Paim
authors.forename: Marta M M F
authors.initials: MM
authors.lastname: Duarte
authors.forename: Verônica
authors.initials: V
authors.lastname: Castro
authors.forename: Heloisa E
authors.initials: HE
authors.lastname: Palma
authors.forename: Raqueli T
authors.initials: RT
authors.lastname: França
authors.forename: Diandra V
authors.initials: DV
authors.lastname: Felin
authors.forename: Lucas C
authors.initials: LC
authors.lastname: Siqueira
authors.forename: Sonia T A
authors.initials: ST
authors.lastname: Lopes
authors.forename: Maria Rosa C
authors.initials: MR
authors.lastname: Schetinger
authors.forename: Silvia G
authors.initials: SG
authors.lastname: Monteiro
authors.forename: Cinthia M
authors.initials: CM
authors.lastname: Mazzanti
mesh_terms: D000110:Acetylcholinesterase / Q000097:blood* / Q000378:metabolism; D000284:Administration, Oral; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology* / Q000627:therapeutic use; D002800:Cholinesterase Inhibitors / Q000494:pharmacology / Q000627:therapeutic use; D003474:Curcumin / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D016207:Cytokines / Q000097:blood* / Q000378:metabolism; D004195:Disease Models, Animal; D007155:Immunologic Factors / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D018512:Parasitemia; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014345:Trypanosoma / Q000187:drug effects / Q000276:immunology; D014352:Trypanosomiasis / Q000188:drug therapy / Q000201:enzymology / Q000276:immunology* / Q000517:prevention & control
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D002800:Cholinesterase Inhibitors; D016207:Cytokines; D007155:Immunologic Factors; D000110:Acetylcholinesterase; D003474:Curcumin