title: Transforming growth factor-β transiently induces vimentin expression and invasive capacity in a canine mammary gland tumor cell line.
abstract: The epithelial-mesenchymal transition (EMT) is a crucial event that occurs during cancer metastasis and can be induced by transforming growth factor-β (TGF-β) in various tumor cells in vitro. However, little is known about the effects of TGF-β in canine mammary gland tumors (CMGTs). Here, we investigated the role of TGF-β in CMGT. We observed that treatment of the CMGT cell line CHMp13a with TGF-β1 leads to transient induction of the mesenchymal marker vimentin. Real-time measurements of cellular electrical impedance also showed that CMGT invasiveness is transiently increased by TGF-β1 treatment, but is reversed after prolonged stimulation. This phenomenon is similar to the mesenchymal-epithelial transition (MET, the reverse phenomenon of EMT), and a process that is implicated in the establishment of secondary metastatic lesions.
authors.affiliation: Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yoshida
authors.forename: T
authors.initials: T
authors.lastname: Saito
authors.forename: A
authors.initials: A
authors.lastname: Kamida
authors.forename: K
authors.initials: K
authors.lastname: Matsumoto
authors.forename: K
authors.initials: K
authors.lastname: Saeki
authors.forename: M
authors.initials: M
authors.lastname: Mochizuki
authors.forename: N
authors.initials: N
authors.lastname: Sasaki
authors.forename: T
authors.initials: T
authors.lastname: Nakagawa
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor / Q000187:drug effects / Q000473:pathology; D004283:Dog Diseases / Q000378:metabolism / Q000473:pathology*; D004285:Dogs; D005260:Female; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015674:Mammary Neoplasms, Animal / Q000378:metabolism / Q000473:pathology*; D009361:Neoplasm Invasiveness; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014746:Vimentin / Q000096:biosynthesis*
chemical_list: D016212:Transforming Growth Factor beta; D014746:Vimentin