title: Enzymatic saccharification of sugarcane bagasse by N-methylmorpholine-N-oxide-tolerant cellulase from a newly isolated Galactomyces sp. CCZU11-1.
abstract: Based on the enrichment culture strategy, a novel N-methylmorpholine-N-oxide (NMMO)-tolerant cellulase-producing strain Galactomyces sp. CCZU11-1 was isolated from soil samples. After the optimization of culture condition, the highest FPA (13.4 U/mL) and CMCase (24.5 U/mL) were obtained. In both culture and reaction media containing NMMO 25% (w/v), the cellulase from Galactomyces sp. CCZU11-1 still had good activity. Furthermore, high saccharification rate was obtained in aqueous-NMMO media. Moreover, the fermentability of the hydrolyzates, obtained after enzymatic in situ saccharification of the NMMO-pretreated sugarcane bagasse, was evaluated using Saccharomyce scerevisiae. In conclusion, Galactomyces sp. CCZU11-1 is a promising candidate as high NMMO-tolerant cellulase producer and has potential application in future.
authors.affiliation: Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou 213164, PR China. yucaihe2007@yahoo.com.cn
authors.forename: Yu-Cai
authors.initials: YC
authors.lastname: He
authors.forename: Dong-Qin
authors.initials: DQ
authors.lastname: Xia
authors.forename: Cui-Luan
authors.initials: CL
authors.lastname: Ma
authors.forename: Lei
authors.initials: L
authors.lastname: Gong
authors.forename: Ting
authors.initials: T
authors.lastname: Gong
authors.forename: Miao-Xin
authors.initials: MX
authors.lastname: Wu
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ya-Jie
authors.initials: YJ
authors.lastname: Tang
authors.forename: Jian-He
authors.initials: JH
authors.lastname: Xu
authors.forename: You-Yan
authors.initials: YY
authors.lastname: Liu
mesh_terms: D001203:Ascomycota / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D003497:Cyclic N-Oxides / Q000494:pharmacology*; D005285:Fermentation / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D009025:Morpholines / Q000494:pharmacology*; D010802:Phylogeny; D031786:Saccharum / Q000378:metabolism*
chemical_list: D003497:Cyclic N-Oxides; D009025:Morpholines; D002482:Cellulose; C027433:bagasse; C067960:4-methylmorpholine N-oxide; D002480:Cellulase