title: Microbial sampling process can change results of microbiological analysis in periodontitis diagnosis. A minireview.
abstract: This minireview aims to verify the supposition that the microbial sampling process can change results of microbiological analysis in periodontitis diagnosis. The literature search via Pubmed yielded 52 appropriate articles for analysis. Of which 38% (20/52) described that the sampling sites were isolated from saliva, whereas 62% (32/52) did not. Also, 29% (15/52) declared that the microbial sampling was performed before probing pocket depth (PPD), whereas 71% (37/52) did not. Comparison of the results of microbiological analysis in these studies showed that the bacteria most frequently detected in periodontal pockets was variable. Therefore, a sampling process that includes both the microbial sample being taken before PPD and saliva isolation of the sampling sites is needed to ensure the accuracy of microbiological analysis in periodontitis diagnosis.
authors.affiliation: Aix-Marseille Université, URMITE, UM63, CNRS 7278, IRD 198, Inserm 1095, Marseille, France. nguyen.hieu.tung@gmail.com
authors.forename: Tung
authors.initials: T
authors.lastname: Nguyen-Hieu
mesh_terms: D010520:Aggressive Periodontitis / Q000175:diagnosis / Q000382:microbiology*; D001421:Bacteria, Anaerobic / Q000302:isolation & purification; D001431:Bacteriological Techniques / Q000379:methods*; D055113:Chronic Periodontitis / Q000175:diagnosis / Q000382:microbiology*; D046508:Culture Techniques; D003773:Dental Plaque / Q000382:microbiology; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010514:Periodontal Pocket / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012463:Saliva / Q000382:microbiology*; D013048:Specimen Handling / Q000379:methods