title: Immunohistochemical analysis of medullary breast carcinoma autoantigens in different histological types of breast carcinomas.
abstract: BACKGROUND
  On the past decade a plethora of investigations were directed on identification of molecules involved in breast tumorogenesis, which could represent a powerful tool for monitoring, diagnostics and treatment of this disease. In current study we analyzed six previously identified medullary breast carcinoma autoantigens including LGALS3BP, RAD50, FAM50A, RBPJ, PABPC4, LRRFIP1 with cancer restricted serological profile in different histological types of breast cancer.
  
  
  METHODS
  Semi-quantitative immunohistochemical analysis of 20 tissue samples including medullary breast carcinoma, invasive ductal carcinoma, invasive lobular carcinoma and non-cancerous tissues obtained from patients with fibrocystic disease (each of five) was performed using specifically generated polyclonal antibodies. Differences in expression patterns were evaluated considering percent of positively stained cells, insensitivity of staining and subcellular localization in cells of all tissue samples.
  
  
  RESULTS
  All 6 antigens predominantly expressed in the most cells of all histological types of breast tumors and non-cancerous tissues with slight differences in intensity of staining and subcellular localization. The most significant differences in expression pattern were revealed for RAD50 and LGALS3BP in different histological types of breast cancer and for PABPC4 and FAM50A antigens in immune cells infiltrating breast tumors.
  
  
  CONCLUSIONS
  This pilot study made possible to select 4 antigens LGALS3BP, RAD50, PABPC4, and FAM50A as promising candidates for more comprehensive research as potential molecular markers for breast cancer diagnostics and therapy.
  The virtual slides' for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1860649350796892.
authors.affiliation: Department of Cell Signaling, Institute of Molecular Biology and Genetics, NAS of Ukraine, 150, Zabolotnogo str., Kyiv, Ukraine. kostjanez@ukr.net
authors.forename: Olga
authors.initials: O
authors.lastname: Kostianets
authors.forename: Stepan
authors.initials: S
authors.lastname: Antoniuk
authors.forename: Valeriy
authors.initials: V
authors.lastname: Filonenko
authors.forename: Ramziya
authors.initials: R
authors.lastname: Kiyamova
mesh_terms: D017766:Acid Anhydride Hydrolases; D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis; D001324:Autoantigens / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D001798:Blood Proteins / Q000032:analysis; D001943:Breast Neoplasms / Q000145:classification / Q000276:immunology* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000145:classification / Q000276:immunology* / Q000473:pathology; D018275:Carcinoma, Lobular / Q000145:classification / Q000276:immunology* / Q000473:pathology; D018276:Carcinoma, Medullary / Q000145:classification / Q000276:immunology* / Q000473:pathology; D002352:Carrier Proteins / Q000032:analysis; D045643:DNA Repair Enzymes / Q000032:analysis; D004268:DNA-Binding Proteins / Q000032:analysis; D005260:Female; D005348:Fibrocystic Breast Disease / Q000276:immunology / Q000473:pathology; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009687:Nuclear Proteins / Q000032:analysis; D010865:Pilot Projects; D039102:Poly(A)-Binding Proteins / Q000032:analysis; D016601:RNA-Binding Proteins; D016326:Extracellular Matrix Proteins
chemical_list: D017766:Acid Anhydride Hydrolases; D000951:Antigens, Neoplasm; D001324:Autoantigens; D014408:Biomarkers, Tumor; D001798:Blood Proteins; D002352:Carrier Proteins; D045643:DNA Repair Enzymes; D004268:DNA-Binding Proteins; D006023:Glycoproteins; D009687:Nuclear Proteins; D039102:Poly(A)-Binding Proteins; D016601:RNA-Binding Proteins; C108827:FAM50A protein, human; C081856:LGALS3BP protein, human; D016326:Extracellular Matrix Proteins; C104581:PABPC4 protein, human; C112158:RAD50 protein, human