title: Interactions between E6AP and E6 proteins from alpha and beta HPV types.
abstract: High-risk mucosotropic Human papillomaviruses (HPVs), especially HPV-16, are the aetiological agents of cervical cancer and the cellular targets of their E6 oncoproteins have been much studied. However, much less is known about the cellular targets of the cutaneous HPV E6 proteins. In this study, a proteomic analysis of cells transfected with the E6 proteins from cutaneous HPV types specifically identified E6-interacting proteins involved in the ubiquitination pathways. These include the E3 ubiquitin-protein ligases E6AP and UBR4/p600. We also show that E6AP can contribute towards the steady-state levels of E6 and, conversely, that certain E6 proteins, in addition to those derived from the high-risk mucosal HPV types, can enhance levels of E6AP turnover. These results define important differences and commonalities in how HPV E6 proteins of mucosal and cutaneous origin interact with cellular ubiquitin-protein ligases.
authors.affiliation: International Centre for Genetic Engineering and Biotechnology, Padriciano 99, Trieste 34149, Italy. miranda@icgeb.org
authors.forename: Miranda
authors.initials: M
authors.lastname: Thomas
authors.forename: Vjekoslav
authors.initials: V
authors.lastname: Tomaić
authors.forename: David
authors.initials: D
authors.lastname: Pim
authors.forename: Michael P
authors.initials: MP
authors.lastname: Myers
authors.forename: Massimo
authors.initials: M
authors.lastname: Tommasino
authors.forename: Lawrence
authors.initials: L
authors.lastname: Banks
mesh_terms: D052685:Alphapapillomavirus / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D052686:Betapapillomavirus / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D040901:Proteomics; D014162:Transfection; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*
chemical_list: D009856:Oncogene Proteins, Viral; D044767:Ubiquitin-Protein Ligases