title: Encapsulation of ligninolytic enzymes and its application in clarification of juice.
abstract: The thermal stability, physico-chemical properties and effect on juice clarification of hydrogel formulations of ligninolytic enzymes from Phanerochaete chrysosporium were evaluated. The results showed that enzyme entrapment increase significantly (P<0.05) the thermal stability of enzymes at 4 and 75 °C. At 75 °C, maximum activity decreased to non detectable values of 7.9% for free laccase, manganese peroxidase (MnP), lignin peroxidase (LiP), respectively; to 94%, 97%, 93% for laccase, MnP and LiP entrapped into Polyacrylamide/pectin, 94%, 98%, 88% for laccase, MnP and LiP encapsulated respectively into polyacrylamide/ gelatine and to 87%, 91%, 87% for laccase, MnP and LiP entrapped, respectively into polyacrylamide/carboxymethylcellulose (CMC). When particle size and viscosity of the formulation increased, enzyme stability increased. The polyphenolic reduction and clarity amelioration in mixed juice of berry and pomegranate was more significant (p>0.05) using encapsulated enzymes treatment than free enzymes. This suggested that enzymatic treatment was efficient for the juice clarification.
authors.affiliation: INRS-ETE, Université du Québec, 490, rue de la Couronne, Québec G1K 9A9, Canada.
authors.forename: Fatma
authors.initials: F
authors.lastname: Gassara-Chatti
authors.forename: Satinder K
authors.initials: SK
authors.lastname: Brar
authors.forename: C M
authors.initials: CM
authors.lastname: Ajila
authors.forename: M
authors.initials: M
authors.lastname: Verma
authors.forename: R D
authors.initials: RD
authors.lastname: Tyagi
authors.forename: J R
authors.initials: JR
authors.lastname: Valero
mesh_terms: D001628:Beverages / Q000032:analysis*; D004795:Enzyme Stability; D005524:Food Technology / Q000379:methods*; D005656:Fungal Proteins / Q000737:chemistry*; D010316:Particle Size; D010544:Peroxidases / Q000737:chemistry*; D020075:Phanerochaete / Q000737:chemistry / Q000201:enzymology*
chemical_list: D005656:Fungal Proteins; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase