title: All-trans-retinoic acid ameliorates experimental allergic encephalomyelitis by affecting dendritic cell and monocyte development.
abstract: Experimental allergic encephalomyelitis (EAE) can be induced in animal models by injecting the MOG35-55 peptide subcutaneously. Dendritic cells (DCs) that are located at the immunization site phagocytose the MOG35-55 peptide. These DCs mature and migrate into the nearest draining lymph nodes (dLNs), then present antigen, resulting in the activation of naive T cells. T helper type 1 (Th1) and Th17 cells are the primary cells involved in EAE progression. All-trans-retinoic acid (AT-RA) has been shown to have beneficial effects on EAE progression; however, whether AT-RA influences DC maturation or mediates other functions is unclear. In the present study, we showed that AT-RA led to the down-regulation of MHC class II, CD80 (B7-1) and CD86 (B7-2) expressed on the surface of DCs that were isolated from dLNs or spleen 3 days post-immunization in an EAE model. Changes to DC function influenced Th1/Th17 subset polarization. Furthermore, the number of CD44(+) monocytes (which might trigger EAE progression) was also significantly decreased in dLNs, spleen, subarachnoid space and the spinal cord parenchyma after AT-RA treatment. These findings are the first to demonstrate that AT-RA impairs the antigen-presenting capacity of DCs, leading to down-regulation of pathogenic Th1 and Th17 inflammatory cell responses and reducing EAE severity.
authors.affiliation: Department of Neurobiology, Harbin Medical University Provincial Key Laboratory of Neurobiology, Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Zhan
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Jin-Feng
authors.initials: JF
authors.lastname: Yang
authors.forename: Guang-You
authors.initials: GY
authors.lastname: Wang
authors.forename: Lili
authors.initials: L
authors.lastname: Mu
authors.forename: Tong-Shuai
authors.initials: TS
authors.lastname: Zhang
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Xie
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Wang
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Liu
authors.forename: Qing-Fei
authors.initials: QF
authors.lastname: Kong
authors.forename: Hu-Lun
authors.initials: HL
authors.lastname: Li
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000187:drug effects; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D002454:Cell Differentiation / Q000187:drug effects; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology; D007114:Immunization; D008198:Lymph Nodes / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; D015398:Signal Transduction / Q000187:drug effects; D013116:Spinal Cord / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D058504:Th17 Cells / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D014212:Tretinoin / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D015703:Antigens, CD; D000975:Antioxidants; D000949:Histocompatibility Antigens Class II; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; C445081:myelin oligodendrocyte glycoprotein (35-55); D014212:Tretinoin