title: Isolation and characterization of microsatellites in a medicinal plant, Phyllanthus emblica (Euphorbiaceae).
abstract: OBJECTIVE
  Microsatellite markers were isolated and characterized in a medicinal plant, Phyllanthus emblica, to study population genetics for designing an effective in situ and ex situ conservation of genetic resources of the species. •
  
  
  RESULTS
  Six microsatellite markers were developed using an enrichment and magnetic separation protocol. They were characterized in two natural populations of P. emblica. Out of the six microsatellites, five showed polymorphism, with the number of alleles ranging from four to seven. Observed and expected heterozygosities ranged from 0.360 to 0.760 and 0.499 to 0.806, respectively. •
  
  
  CONCLUSIONS
  The five polymorphic microsatellite markers will be useful for studying the genetic structure, reproductive biology, and for identification of clones and provenances of this important medicinal plant.
authors.affiliation: Forest Genetics and Biotechnology Division, Forest and Plant Conservation Research Office, National Park, Wildlife and Plant Conservation Department, 61 Phaholyothin, Chatuchak, Bangkok 10900 Thailand. madhav.mpandey@gmail.com
authors.forename: Madhav
authors.initials: M
authors.lastname: Pandey
authors.forename: Suchitra
authors.initials: S
authors.lastname: Changtragoon
mesh_terms: D002467:Cell Nucleus / Q000235:genetics; D017931:DNA Primers / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D029842:Phyllanthus emblica / Q000166:cytology / Q000235:genetics*; D010946:Plants, Medicinal / Q000166:cytology / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012098:Reproduction; D017422:Sequence Analysis, DNA; D013785:Thailand
chemical_list: D017931:DNA Primers; D018744:DNA, Plant