title: Detection of Rickettsia massiliae in Rhipicephalus sanguineus from the eastern United States.
abstract: We report the first evidence of Rickettsia massiliae in the brown dog tick, Rhipicephalus sanguineus, from the East Coast of the United States. As part of routine pathogen surveillance, DNA samples from ixodid ticks were tested for spotted fever group rickettsiae by nested PCR. A R. massiliae-positive tick was collected off a beagle mix recently rescued from North Carolina. Infection was confirmed by partial sequence analysis of the htrA, gltA, ompB, ompA, and sca4 genes, which had 100% identity to a R. massiliae isolate from Arizona.
authors.affiliation: The W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205-2179, USA. cfornadel@gmail.com
authors.forename: Christen M
authors.initials: CM
authors.lastname: Fornadel
authors.forename: Joshua D
authors.initials: JD
authors.lastname: Smith
authors.forename: Sonya E
authors.initials: SE
authors.lastname: Zawada
authors.forename: Jorge R
authors.initials: JR
authors.lastname: Arias
authors.forename: Douglas E
authors.initials: DE
authors.lastname: Norris
mesh_terms: D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D004283:Dog Diseases / Q000382:microbiology / Q000469:parasitology; D004285:Dogs; D062665:Epidemiological Monitoring; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D008297:Male; D008396:Maryland / Q000453:epidemiology; D009657:North Carolina / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D048508:Rhipicephalus sanguineus / Q000382:microbiology*; D012281:Rickettsia / Q000235:genetics / Q000302:isolation & purification*; D012282:Rickettsia Infections / Q000453:epidemiology / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013984:Tick Infestations / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary; D014768:Virginia / Q000453:epidemiology
chemical_list: D004269:DNA, Bacterial