title: Cultivar-specific kinetics of chitinase induction in soybean roots during exposure to arsenic.
abstract: The kinetics of defense responses was studied in soybean exposed to ecologically relevant concentrations of arsenic for 96 h. In the roots of two soybean cultivars with contrasting tolerance to this metalloid there were observed differences in basal levels of membrane lipid peroxidation as well as a significantly different course of peroxidation upon exposure to As. The different course of stress was reflected in the accumulation of defense components. The responses of individual chitinase isoforms were studied since these enzymes had previously been shown to be stable components of defense against metals. The kinetics and magnitude of accumulation of the three isoforms during exposure to As significantly differed within as well as between the studied cultivars. Furthermore, accumulation of these isoforms appeared to be related to oxidative status in the root tissue. The timing of induced responses is likely to be important for efficient defense against metal(oid) pollution in environment.
authors.affiliation: Department of Botany and Genetics, Faculty of Natural Sciences, The Constantine Philosopher University, Nábrežie mládeže 91, 949 74, Nitra, Slovak Republic.
authors.forename: Patrik
authors.initials: P
authors.lastname: Mészáros
authors.forename: L'ubomír
authors.initials: L
authors.lastname: Rybanský
authors.forename: Pavol
authors.initials: P
authors.lastname: Hauptvogel
authors.forename: Roman
authors.initials: R
authors.lastname: Kuna
authors.forename: Jana
authors.initials: J
authors.lastname: Libantová
authors.forename: Jana
authors.initials: J
authors.lastname: Moravčíková
authors.forename: Beáta
authors.initials: B
authors.lastname: Piršelová
authors.forename: Anna
authors.initials: A
authors.lastname: Tirpáková
authors.forename: Ildikó
authors.initials: I
authors.lastname: Matušíková
mesh_terms: D001151:Arsenic / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002688:Chitinases / Q000378:metabolism*; D004361:Drug Tolerance; D006861:Hydrogen Peroxide / Q000378:metabolism; D007527:Isoenzymes; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects; D018517:Plant Roots / Q000187:drug effects* / Q000378:metabolism*; D040901:Proteomics; D013025:Glycine max / Q000187:drug effects* / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D007527:Isoenzymes; D006861:Hydrogen Peroxide; D002688:Chitinases; D001151:Arsenic