title: Feeding and breeding aspects of Stomoxys calcitrans (Diptera: Muscidae) under laboratory conditions.
abstract: Bionomic aspects of Stomoxys calcitrans (Linnaeus, 1758) (Diptera: Muscidae) were studied under laboratory conditions. For this reason, laboratory-rearing techniques were optimized at the National Veterinary School of Toulouse. The colony was maintained at 25±2°C, 50±10% RH under a 12-hour light cycle and observed daily. The size of each adult cage is 30x30x30 cm and designed to house about 500-1,000 flies. The average cycle from egg to adult was 19.2±1.7 days. The mean longevity of imagos was 9.3±5.8 days and not significantly different between sexes. Stable flies were split into two groups; the first was fed with blood, honey and water, and the second was fed only with honey and water. The mean weight of a blood meal was 11.1±3.8 mg with no significant differences between males and females. The mean longevity of non-blood fed flies was found to be significantly higher (10.4±3.9 days) than those fed with blood. The maximum lifespan was shorter for non-blood fed males (17 days) and females (18 days) than for those fed with blood (females: 24 days, males: 23 days). Under these laboratory conditions, S. calcitrans rearing was successfully established. In the end, the number of expected generations of S. calcitrans and the net reproduction rate were estimated to be 11.8 generations/year and 16.2 living females per female respectively.
authors.affiliation: Laboratoire de Parasitologie, Université de Toulouse, INP, ENVT, Toulouse, France.
authors.forename: A
authors.initials: A
authors.lastname: Salem
authors.forename: M
authors.initials: M
authors.lastname: Franc
authors.forename: P
authors.initials: P
authors.lastname: Jacquiet
authors.forename: E
authors.initials: E
authors.lastname: Bouhsira
authors.forename: E
authors.initials: E
authors.lastname: Liénard
mesh_terms: D000821:Animal Feed; D000818:Animals; D001769:Blood; D005260:Female; D005298:Fertility; D006722:Honey; D008297:Male; D009117:Muscidae / Q000254:growth & development / Q000502:physiology*; D012098:Reproduction; D013997:Time Factors; D014867:Water
chemical_list: D014867:Water