title: Human cord blood γδ T cells expressing public Vγ2 chains dominate the response to bisphosphonate plus interleukin-15.
abstract: Compared with adults, the circulating Vγ2Vδ2 T-cell population in cord blood is present at low levels and does not show the strong bias for Vγ2-Jγ1.2 rearrangements. These features may be a result of limited exposure to stimulatory phosphoantigens, lack of T-cell-derived interleukin-2 (IL-2) or both. In cord blood mononuclear cell cultures, a single round of stimulation, using aminobisphosphonates to elevate phosphoantigen levels, resulted in expansion of adult-like Vγ2 chains and accumulation of memory cells with cytotoxic potential. Selection was similar using IL-2 or myeloid-derived IL-15. The Vγ2Vδ2 T cells present in neonates are capable of generating potent immune responses even when relying on IL-15.
authors.affiliation: Institute of Human Virology, University of Maryland Baltimore, Baltimore, MD, USA.
authors.forename: Cristiana
authors.initials: C
authors.lastname: Cairo
authors.forename: Bertrand
authors.initials: B
authors.lastname: Sagnia
authors.forename: Giulia
authors.initials: G
authors.lastname: Cappelli
authors.forename: Vittorio
authors.initials: V
authors.lastname: Colizzi
authors.forename: Rose G F
authors.initials: RG
authors.lastname: Leke
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Leke
authors.forename: C David
authors.initials: CD
authors.lastname: Pauza
mesh_terms: D000328:Adult; D019386:Alendronate / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D019409:Interleukin-15 / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics* / Q000276:immunology; D011994:Recombinant Proteins / Q000494:pharmacology; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000187:drug effects* / Q000276:immunology
chemical_list: D019409:Interleukin-15; D007376:Interleukin-2; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011994:Recombinant Proteins; D019386:Alendronate