title: In vitro antigen-specific induction of IL-22 in human subjects that resolved HCV infection.
abstract: AIMS: To determine if in vitro production of IL-22 and IL-17 correlated with resolution of HCV infection.  MATERIALS #ENTITYSTARTX00026; METHODS: Human peripheral blood cells isolated from a well-defined cohort of resolved and chronic HCV-infected subjects were used to measure HCV-, influenza- and mitogen-activated T-cell proliferation. In addition, IL-22 and IL-17 production was measured via ELISAs and flow cytometry.  RESULTS: Resolved HCV subjects had a significantly higher T-cell proliferative response to recombinant NS3 protein compared with chronic HCV subjects. Resolved subjects had a dose-dependent IL-22 response to recombinant NS3 compared with chronic HCV subjects.  CONCLUSION: IL-22 production is associated with antigen-specific induction of CD4 (+) T cells in individuals that resolved HCV infection, suggesting a potential role for IL-22 in HCV clearance.
authors.affiliation: Department of Pathology, University of Utah School of Medicine, 30 North 1900 East, 3R330 SOM, Salt Lake City, UT 84132, USA.
authors.forename: Matthew F
authors.initials: MF
authors.lastname: Cusick
authors.forename: Jane E
authors.initials: JE
authors.lastname: Libbey
authors.forename: Robert S
authors.initials: RS
authors.lastname: Fujinami
authors.forename: David D
authors.initials: DD
authors.lastname: Eckels