title: Effect of red wines with different in vitro antioxidant activity on oxidative stress of high-fat diet rats.
abstract: It is not known if there is a correlation between in vitro and in vivo antioxidant activity of wines. Thus, in the present study, rats were fed with a high-fat diet to induce inflammation and oxidative stress, and received supplementation (1.0 mL/day) containing water or three red wine samples characterised by high (41.1 mmol TE/L), medium (35.7 mmol TE/L) and low (14.4 mmol TE/L) in vitro antioxidant activity. A correlation was observed between in vitro antioxidant activity measured in the wines and in vivo antioxidant activity measured in plasma (r=+0.49, p=0.011). In liver, only the wine containing the highest in vitro antioxidant activity was able to reduce malondialdehyde concentration. Groups supplemented with wines containing lower in vitro antioxidant activity showed an increase in antioxidant enzyme activity. We concluded that the correlation between in vitro and in vivo antioxidant activity depends on the biomarker selected to evaluate the oxidative stress.
authors.affiliation: LADAF, Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of São Paulo, NAPAN-Food and Nutrition Research Center, Av. Lineu Prestes 580, 05508-900 São Paulo, Brazil.
authors.forename: Luciene Fagundes Lauer
authors.initials: LF
authors.lastname: Macedo
authors.forename: Marcelo Macedo
authors.initials: MM
authors.lastname: Rogero
authors.forename: Jessica Pereira
authors.initials: JP
authors.lastname: Guimarães
authors.forename: Daniel
authors.initials: D
authors.lastname: Granato
authors.forename: Luciana Pereira
authors.initials: LP
authors.lastname: Lobato
authors.forename: Inar Alves
authors.initials: IA
authors.lastname: Castro
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects*; D004041:Dietary Fats / Q000008:administration & dosage / Q000378:metabolism*; D019587:Dietary Supplements; D006801:Humans; D007249:Inflammation / Q000178:diet therapy / Q000209:etiology / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D014920:Wine / Q000032:analysis*
chemical_list: D000975:Antioxidants; D004041:Dietary Fats; D008315:Malondialdehyde