title: Second double-stranded RNA binding domain of dicer-like ribonuclease 1: structural and biochemical characterization.
abstract: Dicer-like ribonuclease III enzymes are involved in different paths related to RNA silencing in plants. Little is known about the structural aspects of these processes. Here we present a structural characterization of the second double-stranded RNA binding domain (dsRBD) of DCL1, which is presumed to participate in pri-micro-RNA recognition and subcellular localization of this protein. We determined the solution structure and found that it has a canonical fold but bears some variation with respect to other homologous domains. We also found that this domain binds both double-stranded RNA and double-stranded DNA, in contrast to most dsRBDs. Our characterization shows that this domain likely has functions other than substrate recognition and binding.
authors.affiliation: Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Santa Fe, Argentina.
authors.forename: Paula
authors.initials: P
authors.lastname: Burdisso
authors.forename: Irina P
authors.initials: IP
authors.lastname: Suarez
authors.forename: Nicolás G
authors.initials: NG
authors.lastname: Bologna
authors.forename: Javier F
authors.initials: JF
authors.lastname: Palatnik
authors.forename: Beate
authors.initials: B
authors.lastname: Bersch
authors.forename: Rodolfo M
authors.initials: RM
authors.lastname: Rasia
mesh_terms: D017360:Arabidopsis / Q000201:enzymology; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000378:metabolism; D053487:DEAD-box RNA Helicases / Q000737:chemistry; D004247:DNA / Q000378:metabolism; D035683:MicroRNAs / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D017434:Protein Structure, Tertiary; D012330:RNA, Double-Stranded / Q000378:metabolism*; D043244:Ribonuclease III / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; D035683:MicroRNAs; D012330:RNA, Double-Stranded; D004247:DNA; C402383:DCL1 protein, Arabidopsis; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases