title: Coupled TLC and MALDI-TOF/MS analyses of the lipid extract of the hyperthermophilic archaeon Pyrococcus furiosus.
abstract: The lipidome of the marine hyperthermophilic archaeon Pyrococcus furiosus was studied by means of combined thin-layer chromatography and MALDI-TOF/MS analyses of the total lipid extract. 80-90% of the major polar lipids were represented by archaeol lipids (diethers) and the remaining part by caldarchaeol lipids (tetraethers). The direct analysis of lipids on chromatography plate showed the presence of the diphytanylglycerol analogues of phosphatidylinositol and phosphatidylglycerol, the N-acetylglucosamine-diphytanylglycerol phosphate plus some caldarchaeol lipids different from those previously described. In addition, evidence for the presence of the dimeric ether lipid cardiolipin is reported, suggesting that cardiolipins are ubiquitous in archaea.
authors.affiliation: Department of Basic Medical Sciences, University of Bari Aldo Moro, Piazza G. Cesare, Bari, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Lobasso
authors.forename: Patrizia
authors.initials: P
authors.lastname: Lopalco
authors.forename: Roberto
authors.initials: R
authors.lastname: Angelini
authors.forename: Rita
authors.initials: R
authors.lastname: Vitale
authors.forename: Harald
authors.initials: H
authors.lastname: Huber
authors.forename: Volker
authors.initials: V
authors.lastname: Müller
authors.forename: Angela
authors.initials: A
authors.lastname: Corcelli
mesh_terms: D002855:Chromatography, Thin Layer / Q000379:methods; D008055:Lipids / Q000032:analysis* / Q000302:isolation & purification*; D020027:Pyrococcus furiosus / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D008055:Lipids