title: The protective effect of modified intravenous immunoglobulin in LPS sepsis model is associated with an increased IRA B cells response.
abstract: Intravenous immunoglobulin preparations (IVIg) that have undergone a mild oxidizing treatment with ferrous ions have an increased polyspecificity, which is not associated with a higher propensity to form aggregates. Among other biological properties of the modified IVIg, a protective effect in LPS sepsis model stands out as the native preparation is totally devoid of it or even exacerbates sepsis. A recent finding identified an LPS induced subset of B1 lymphocytes that migrate from the peritoneal cavity to the spleen acquiring the expression of CD93, GM-CSF as well as the capacity to control sepsis. This report demonstrates that modified IVIg, but not the native preparation, causes a further increase in this population during LPS sepsis. Partial targeted suppression of the peritoneal B cell proliferation by an intracellular dye abrogates this effect and the clinical benefit of modified IVIg.
authors.affiliation: Department of Immunology, Stefan Angelov Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
authors.forename: Iglika
authors.initials: I
authors.lastname: Djoumerska-Alexieva
authors.forename: Shina
authors.initials: S
authors.lastname: Pashova
authors.forename: Tchavdar
authors.initials: T
authors.lastname: Vassilev
authors.forename: Anastas
authors.initials: A
authors.lastname: Pashov
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D002478:Cells, Cultured; D016756:Immunoglobulins, Intravenous / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D007155:Immunologic Factors / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D007501:Iron / Q000737:chemistry; D008070:Lipopolysaccharides; D051379:Mice; D008813:Mice, Inbred ICR; D010529:Peritoneal Cavity / Q000166:cytology; D018805:Sepsis / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology
chemical_list: D016756:Immunoglobulins, Intravenous; D007155:Immunologic Factors; D008070:Lipopolysaccharides; D007501:Iron