title: Methionine sulfoxide reduction in ciliates: characterization of the ready-to-use methionine sulfoxide-R-reductase genes in Euplotes.
abstract: Genes encoding the enzyme methionine sulfoxide reductase type B, specific to the reduction of the oxidized methionine-R form, were characterized from the expressed (macronuclear) genome of two ecologically separate marine species of Euplotes, i.e. temperate water E. raikovi and polar water E. nobilii. Both species were found to contain a single msrB gene with a very simple structural organization encoding a protein of 127 (E. raikovi) or 126 (E. nobilii) amino acid residues that belongs to the group of zinc-containing enzymes. Both msrB genes are constitutively expressed, suggesting that the MsrB enzyme plays an essential role in repairing oxidative damages that appear to be primarily caused by physiological cell aging in E. raikovi and by interactions with an O(2) saturated environment in E. nobilii.
authors.affiliation: Dipartimento di Scienze Ambientali e Naturali, University of Camerino, 62032 Camerino (MC), Italy.
authors.forename: Nicoleta
authors.initials: N
authors.lastname: Dobri
authors.forename: Eugenie Emilie Ngueng
authors.initials: EE
authors.lastname: Oumarou
authors.forename: Claudio
authors.initials: C
authors.lastname: Alimenti
authors.forename: Claudio
authors.initials: C
authors.lastname: Ortenzi
authors.forename: Pierangelo
authors.initials: P
authors.lastname: Luporini
authors.forename: Adriana
authors.initials: A
authors.lastname: Vallesi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D016812:Euplotes / Q000201:enzymology* / Q000235:genetics*; D015870:Gene Expression; D008715:Methionine / Q000031:analogs & derivatives* / Q000378:metabolism; D051150:Methionine Sulfoxide Reductases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D011487:Protein Conformation; D016415:Sequence Alignment
chemical_list: D008715:Methionine; D051150:Methionine Sulfoxide Reductases; C013111:methionine sulfoxide