title: Mutual relationships between transcription and pre-mRNA processing in the synthesis of mRNA.
abstract: The generation of messenger RNA (mRNA) in eukaryotes is achieved by transcription from the DNA template and pre-mRNA processing reactions of capping, splicing, and polyadenylation. Although RNA polymerase II (RNAPII) catalyzes the synthesis of pre-mRNA, it also serves as a principal coordinator of the processing reactions in the course of transcription. In this review, we focus on the interplay between transcription and cotranscriptional pre-mRNA maturation events, mediated by the recruitment of RNA processing factors to differentially phosphorylated C-terminal domain of Rbp1, the largest subunit of RNAPII. Furthermore, we highlight the bidirectional nature of the interplay by discussing the impact of RNAPII kinetics on pre-mRNA processing as well as how the processing events reach back to different phases of gene transcription.
authors.affiliation: Institute of Biomedicine, Biochemistry and Developmental Biology, University of Helsinki, Helsinki, Finland. tlenasi@yahoo.com
authors.forename: Tina
authors.initials: T
authors.lastname: Lenasi
authors.forename: Matjaz
authors.initials: M
authors.lastname: Barboric
mesh_terms: D000818:Animals; D006801:Humans; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D012322:RNA Precursors; D012333:RNA, Messenger