title: [Evaluation of the dynamics of drug delivery systems (DDS) using intravital real-time confocal laser scanning microscopy].
abstract: The concept of drug delivery systems (DDS) promises the treatments for the refractory diseases such as cancer. Many kinds of multifunctional DDS have been developed, demonstrating enhanced drug accumulation and therapeutic efficiency. However, it is quite difficult to evaluate that the DDS really perform as expected under in vivo conditions. We have recently developed the intravital real-time confocal laser scanning microscopy (IVRTCLSM) characterized by rapid scanning and simultaneous multicolor detection, to visualize the behavior of DDS in living mice. IVRTCLSM revealed the dynamic states of DDS, which could not be observed by conventional in vitro/ex vivo methods, in the bloodstream, tumors, and other organs. IVRTCLSM will provide new facets in the DDS study, and will facilitate the development of DDS.
authors.affiliation: Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nomoto
authors.forename: Yu
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Kazuko
authors.initials: K
authors.lastname: Toh
authors.forename: R James
authors.initials: RJ
authors.lastname: Christie
authors.forename: Kanjiro
authors.initials: K
authors.lastname: Miyata
authors.forename: Makoto
authors.initials: M
authors.lastname: Oba
authors.forename: Horacio
authors.initials: H
authors.lastname: Cabral
authors.forename: Mami
authors.initials: M
authors.lastname: Murakami
authors.forename: Shigeto
authors.initials: S
authors.lastname: Fukushima
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Nishiyama
authors.forename: Kazunori
authors.initials: K
authors.lastname: Kataoka
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000378:metabolism*; D004337:Drug Carriers; D016503:Drug Delivery Systems; D055808:Drug Discovery; D004431:Ear, External; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D008823:Micelles; D018613:Microscopy, Confocal; D057054:Molecular Imaging / Q000379:methods*; D009369:Neoplasms / Q000378:metabolism*; D010316:Particle Size; D011092:Polyethylene Glycols; D011108:Polymers; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000378:metabolism; D012867:Skin / Q000098:blood supply; D014680:Veins
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D008823:Micelles; D011108:Polymers; D034741:RNA, Small Interfering; D011092:Polyethylene Glycols