title: Divalent cations enhance fluoride binding to Streptococcus mutans and Streptococcus sanguinis cells and subsequently inhibit bacterial acid production.
abstract: One preventive effect of topical fluoride application is derived from the fact that fluoride can inhibit bacterial acid production. Furthermore, divalent cations such as Ca(2+) and Mg(2+) increase the binding of fluoride to bacterial cells. These findings suggest that exposure of oral bacteria to fluoride in the presence of divalent cations increases fluoride binding to bacterial cells and subsequently enhances fluoride-induced inhibition of bacterial acid production. This study investigated the effects of fluoride exposure (0-20,000 ppm F) in the presence of Ca(2+) or Mg(2+) prior to glucose challenge on pH fall ability by bacterial sugar fermentation, as well as fluoride binding to bacterial cells by exposure to fluoride, and fluoride release from bacterial cells during bacterial sugar fermentation, using caries-related bacteria, Streptococcus mutans and Streptococcus sanguinis. The pH fall by both streptococci was inhibited by exposure to over 250 ppm F in the presence of Ca(2+) (p < 0.01), whereas in the presence of Mg(2+), the pH fall by S. mutans and S. sanguinis was inhibited after exposure to over 250 and 950 ppm F, respectively (p < 0.05). The amounts of fluoride binding to and released from streptococcal cells increased with the concentration of fluoride the cells were exposed to in the presence of Mg(2+), but were high enough even after 250 ppm F exposure in the presence of Ca(2+). The enhanced inhibition of acid production in the presence of divalent cations is probably due to the improved efficiency of fluoride binding to bacterial cells being improved via these divalent cations.
authors.affiliation: Division of Pediatric Dentistry, Department of Lifelong Oral Health Science, Tohoku University Graduate School of Dentistry, Sendai, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Domon-Tawaraya
authors.forename: K
authors.initials: K
authors.lastname: Nakajo
authors.forename: J
authors.initials: J
authors.lastname: Washio
authors.forename: T
authors.initials: T
authors.lastname: Ashizawa
authors.forename: T
authors.initials: T
authors.lastname: Ichino
authors.forename: H
authors.initials: H
authors.lastname: Sugawara
authors.forename: S
authors.initials: S
authors.lastname: Fukumoto
authors.forename: N
authors.initials: N
authors.lastname: Takahashi
mesh_terms: D000143:Acids / Q000037:antagonists & inhibitors; D001431:Bacteriological Techniques; D002118:Calcium / Q000494:pharmacology*; D002122:Calcium Chloride / Q000494:pharmacology; D002327:Cariostatic Agents / Q000493:pharmacokinetics* / Q000494:pharmacology; D002413:Cations, Divalent / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005285:Fermentation / Q000187:drug effects; D005459:Fluorides / Q000493:pharmacokinetics* / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017736:Ion-Selective Electrodes; D008274:Magnesium / Q000494:pharmacology*; D015636:Magnesium Chloride / Q000494:pharmacology; D050296:Microbial Viability / Q000187:drug effects; D013295:Streptococcus mutans / Q000187:drug effects / Q000378:metabolism*; D013298:Streptococcus sanguis / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000143:Acids; D002327:Cariostatic Agents; D002413:Cations, Divalent; D015636:Magnesium Chloride; D008274:Magnesium; D005947:Glucose; D002122:Calcium Chloride; D005459:Fluorides; D002118:Calcium