title: Ethanolic extract of propolis, chrysin, CAPE inhibit human astroglia cells.
abstract: OBJECTIVE
  The aim of the present study was to examine the effect of freeze dried ethanolic extract of propolis (EEP), chrysin and caffeic acid phenethyl ester (CAPE) dependently on their concentrations on the viability and morphology of human astroglia cells line (SVGp12).
  
  
  METHODS
  Using gas chromatography - mass spectroscopy (GC-MS) we have established the composition of lyophilisate of EEP collected in Podlasie region (Poland). After 24 h, 48 h and 72 h of exposition to EEP or its ingredients we evaluated the survivability of human astroglia cells (SVGp12) using MTT test. Morphological analysis of human astroglia cells was defined by transmission electron microscope.
  
  
  RESULTS
  About 70 ingredients of EEP were evaluated by GC-MS. We obtained the strong decline of viability of astroglia cells SVGp12 approximately to 16% after EEP; 33% after chrysin and 25% after CAPE application. Condensed form of mitochondria observed in transmission electron microscope may indicate activation of intrinsic pathway of apoptosis induced by EEP, chrysin and CAPE in SVGp12 cell line.
  
  
  CONCLUSIONS
  This study showed that EEP, chrysin and CAPE reduced viability of human astroglia cells probably due to apoptosis process.
authors.affiliation: Department of Bromatology, Medical University of Bialystok, Bialystok, Poland.
authors.forename: R
authors.initials: R
authors.lastname: Markiewicz-Żukowska
authors.forename: H
authors.initials: H
authors.lastname: Car
authors.forename: S K
authors.initials: SK
authors.lastname: Naliwajko
authors.forename: D
authors.initials: D
authors.lastname: Sawicka
authors.forename: B
authors.initials: B
authors.lastname: Szynaka
authors.forename: L
authors.initials: L
authors.lastname: Chyczewski
authors.forename: V
authors.initials: V
authors.lastname: Isidorov
authors.forename: M H
authors.initials: MH
authors.lastname: Borawska
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001253:Astrocytes / Q000187:drug effects* / Q000473:pathology; D002109:Caffeic Acids / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003603:Cytotoxins / Q000633:toxicity; D000431:Ethanol; D005419:Flavonoids / Q000633:toxicity*; D006801:Humans; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000633:toxicity; D011429:Propolis / Q000302:isolation & purification / Q000633:toxicity*
chemical_list: D002109:Caffeic Acids; D003603:Cytotoxins; D005419:Flavonoids; C043561:chrysin; D000431:Ethanol; D011429:Propolis; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol