title: The effects of α-lipoic acid against testicular ischemia-reperfusion injury in Rats.
abstract: Testicular torsion is one of the urologic emergencies occurring frequently in neonatal and adolescent period. Testis is sensitive to ischemia-reperfusion injury, and, therefore, ischemia and consecutive reperfusion cause an enhanced formation of reactive oxygen species that result in testicular cell damage and apoptosis. α-lipoic acid is a free radical scavenger and a biological antioxidant. It is widely used in the prevention of oxidative stress and cellular damage. We aimed to investigate the protective effect of α-lipoic acid on testicular damage in rats subjected to testicular ischemia-reperfusion injury. 35 rats were randomly divided into 5 groups: control, sham operated, ischemia, ischemia-reperfusion, and ischemia-reperfusion +lipoic acid groups, 2 h torsion and 2 h detorsion of the testis were performed. Testicular cell damage was examined by H-E staining. TUNEL and active caspase-3 immunostaining were used to detect germ cell apoptosis. GPx , SOD activity, and MDA levels were evaluated. Histological evaluation showed that α-lipoic acid pretreatment reduced testicular cell damage and decreased TUNEL and caspase-3-positive cells. Additionally, α-lipoic acid administration decreased the GPx and SOD activity and increased the MDA levels. The present results suggest that LA is a potentially beneficial agent in protecting testicular I/R in rats.
authors.affiliation: Department of Histology and Embryology, School of Medicine, Dokuz Eylül University Inciralti, 35340 İzmir, Turkey. seda.ozbal@deu.edu.tr
authors.forename: Seda
authors.initials: S
authors.lastname: Ozbal
authors.forename: Bekir Ugur
authors.initials: BU
authors.lastname: Ergur
authors.forename: Guven
authors.initials: G
authors.lastname: Erbil
authors.forename: Isıl
authors.initials: I
authors.lastname: Tekmen
authors.forename: Alper
authors.initials: A
authors.lastname: Bagrıyanık
authors.forename: Zahide
authors.initials: Z
authors.lastname: Cavdar
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D020287:In Situ Nick-End Labeling; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism; D013086:Spermatic Cord Torsion / Q000188:drug therapy / Q000378:metabolism; D013733:Testicular Diseases / Q000188:drug therapy / Q000378:metabolism; D008063:Thioctic Acid / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D008063:Thioctic Acid; D053148:Caspase 3