title: Immunomodulatory effects of Liriope platyphylla water extract on lipopolysaccharide-activated mouse macrophage.
abstract: The tuber of Liriope platyphylla Wang et Tang (Liliaceae), also known as Liriopis tuber, is famous in Oriental medicine owing to its tonic, antitussive, expectorant and anti-asthmatic properties. In the present study, the effects of Liriopis tuber water extract (LP) on proinflammatory mediators secreted from lipopolysaccharide  (LPS)-induced cultured RAW 264.7 mouse macrophages were investigated. Nitric oxide (NO), prostaglandin E2 (PGE2) and intracellular calcium release were measured after 24 h incubation. Various cytokines and nuclear transcription factors (NF-&#954;B and CREB) of LPS-induced RAW 264.7 were measured by a multiplex bead array assay based on xMAP technology. LP (up to 200 &#956;g/mL) significantly decreased levels of nitric oxide (NO), interleukin (IL)-6, IL-10, IL-12p40, interferon-inducible protein-10, keratinocyte-derived chemokine, monocyte chemotactic protein-1, vascular endothelial growth factor, granulocyte macrophage-colony stimulating factor, platelet derived growth factor, PGE2, intracellular calcium, NF-&#954;B and CREB in LPS-induced RAW 264.7 cells (p &lt; 0.05). The results suggest that LP has immunomodulatory activity to reduce excessive immune reactions during the activation of macrophages by LPS. Further studies are needed to verify the precise mechanism regulating immunomodulatory activities of LP.
authors.affiliation: College of Korean Medicine, Gachon University, Seongnam, Korea. doin303@hanmail.net
authors.forename: Hye Kyung
authors.initials: HK
authors.lastname: Kim
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Hyo-Sang
authors.initials: HS
authors.lastname: Han
authors.forename: Young-Jin
authors.initials: YJ
authors.lastname: Kim
authors.forename: Hyun Joo
authors.initials: HJ
authors.lastname: Kim
authors.forename: Yoon-Sang
authors.initials: YS
authors.lastname: Kim
authors.forename: Hyung Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Seong-Gyu
authors.initials: SG
authors.lastname: Ko
authors.forename: Hyo-Jin
authors.initials: HJ
authors.lastname: An
authors.forename: Young-Jong
authors.initials: YJ
authors.lastname: Lee
authors.forename: Wansu
authors.initials: W
authors.lastname: Park
mesh_terms: D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007155:Immunologic Factors / Q000494:pharmacology*; D018836:Inflammation Mediators / Q000378:metabolism*; D019668:Liliaceae; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D035281:Plant Tubers
chemical_list: D016207:Cytokines; D004365:Drugs, Chinese Herbal; D007155:Immunologic Factors; D018836:Inflammation Mediators; D008070:Lipopolysaccharides