title: A unique feature of iron loss via close adhesion of Helicobacter pylori to host erythrocytes.
abstract: Iron deficiency anemia is an extra-stomach disease experienced in H. pylori carriers. Individuals with type A blood are more prone to suffering from H. pylori infection than other individuals. To clarify the molecular mechanisms underlying H. pylori-associated anemia, we collected erythrocytes from A, B, O, and AB blood donors and analyzed morphology, the number of erythrocytes with H. pylori colonies attached to them, and iron contents in erythrocytes and H. pylori (NCTC11637 and SS1 strains) by means of optical microscopy, scanning electron microscopy, and synchrotron radiation soft X-ray imaging. The number of type A erythrocytes with H. pylori attached to them was significantly higher than that of other erythrocytes (P<0.05). Far more iron distribution was observed in H. pylori bacteria using dual energy analysis near the iron L2, 3 edges by soft X-ray imaging. Iron content was significantly reduced in host erythrocytes after 4 hours of exposure to H. pylori. H. pylori are able to adhere more strongly to type A erythrocytes, and this is related to iron shift from the host to the bacteria. This may explain the reasons for refractory iron deficiency anemia and elevated susceptibility to H. pylori infection in individuals with type A blood.
authors.affiliation: Shanghai Institute of Digestive Surgery and Department of Surgery, Shanghai Ruijin Hospital, Shanghai Key Laboratory for Gastric Neoplasia, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Wang
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Guo
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Ji
authors.forename: Jianian
authors.initials: J
authors.lastname: Zhang
authors.forename: Xuehua
authors.initials: X
authors.lastname: Chen
authors.forename: Bingya
authors.initials: B
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Qiulan
authors.initials: Q
authors.lastname: Ding
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Zhenggang
authors.initials: Z
authors.lastname: Zhu
authors.forename: Yingyan
authors.initials: Y
authors.lastname: Yu
mesh_terms: D000017:ABO Blood-Group System / Q000378:metabolism*; D018798:Anemia, Iron-Deficiency / Q000378:metabolism / Q000382:microbiology; D001422:Bacterial Adhesion; D002478:Cells, Cultured; D015169:Colony Count, Microbial; D004912:Erythrocytes / Q000378:metabolism* / Q000382:microbiology; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D007501:Iron / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D009928:Organ Specificity; D017356:Synchrotrons; D056928:X-Ray Absorption Spectroscopy
chemical_list: D000017:ABO Blood-Group System; D007501:Iron