title: Effects of chronic caloric restriction on kidney and heart redox status and antioxidant enzyme activities in Wistar rats.
abstract: Caloric restriction (CR) has been associated with health benefits and these effects have been attributed, in part, to modulation of oxidative status by CR; however, data are still controversial. Here, we investigate the effects of seventeen weeks of chronic CR on parameters of oxidative damage/ modification of proteins and on antioxidant enzyme activities in cardiac and kidney tissues. Our results demonstrate that CR induced an increase in protein carbonylation in the heart without changing the content of sulfhydryl groups or the activities of superoxide dismutase and catalase (CAT). Moreover, CR caused an increase in CAT activity in kidney, without changing other parameters. Protein carbonylation has been associated with oxidative damage and functional impairment; however, we cannot exclude the possibility that, under our conditions, this alteration indicates a different functional meaning in the heart tissue. In addition, we reinforce the idea that CR can increase CAT activity in the kidney.
authors.affiliation: Departamento de Bioquímica, Instituto de Ciências Básicas da Sáude, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. marciodutra5@yahoo.com.br
authors.forename: Márcio Ferreira
authors.initials: MF
authors.lastname: Dutra
authors.forename: Ivi Juliana
authors.initials: IJ
authors.lastname: Bristot
authors.forename: Cristiane
authors.initials: C
authors.lastname: Batassini
authors.forename: Núbia Broetto
authors.initials: NB
authors.lastname: Cunha
authors.forename: Adriana Fernanda Kuckartz
authors.initials: AF
authors.lastname: Vizuete
authors.forename: Daniela Fraga
authors.initials: DF
authors.lastname: de Souza
authors.forename: José Cláudio Fonseca
authors.initials: JC
authors.lastname: Moreira
authors.forename: Carlos-Alberto
authors.initials: CA
authors.lastname: Gonçalves
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D031204:Caloric Restriction; D002374:Catalase / Q000378:metabolism; D006321:Heart / Q000503:physiopathology*; D007668:Kidney / Q000503:physiopathology*; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D050050:Protein Carbonylation; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002374:Catalase; D013482:Superoxide Dismutase