title: Chemical composition and efficacy of dichloromethane extract of Croton sphaerogynus Baill. (Euphorbiaceae) against the cattle tick Rhipicephalus microplus (Acari: Ixodidae).
abstract: The cattle tick Rhipicephalus microplus is widely distributed in tropical and subtropical regions, causing high economic impact on cattle production. The control of tick infestations is regarded worldwide as critical and has been based on the use of organophosphates, synthetic pyretroids, amitraz and recently ivermectin and fipronil. The present study reports the analysis by gas chromatography/mass spectrometry of the constituents of leaf extracts of Croton sphaerogynus and results of acaricidal activity against the cattle tick R. microplus. The larval package test using the serial dilutions 0.625%, 1.25%, 2.5%, 5.0%, 10.0% and 20.0% (v/v) gave mortality rates 2.25%, 8.26%, 8.81%, 24.80%, 83.66% and 99.32%, respectively. Relevant constituents identified were abietanes, podocarpenes and clerodane type furano diterpenes. The present work may represent a possibility of attainment of natural substances useful for the control of R. microplus.
authors.affiliation: Botany Department, Institute of Biosciences, University of São Paulo, Brazil. adnerighi@gmail.com
authors.forename: Adne A
authors.initials: AA
authors.lastname: Righi
authors.forename: Lucimar B
authors.initials: LB
authors.lastname: Motta
authors.forename: Guilherme M
authors.initials: GM
authors.lastname: Klafke
authors.forename: Paula C
authors.initials: PC
authors.lastname: Pohl
authors.forename: Cláudia M
authors.initials: CM
authors.lastname: Furlan
authors.forename: Deborah Y A C
authors.initials: DY
authors.lastname: Santos
authors.forename: Maria L F
authors.initials: ML
authors.lastname: Salatino
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Negri
authors.forename: Marcelo B
authors.initials: MB
authors.lastname: Labruna
authors.forename: Antonio
authors.initials: A
authors.lastname: Salatino
mesh_terms: D056810:Acaricides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D001681:Biological Assay / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy* / Q000469:parasitology; D029802:Croton / Q000737:chemistry*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry / Q000662:veterinary; D007814:Larva; D008752:Methylene Chloride; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal; D048494:Rhipicephalus / Q000187:drug effects* / Q000502:physiology; D013984:Tick Infestations / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary*
chemical_list: D056810:Acaricides; D004224:Diterpenes; D010936:Plant Extracts; D008752:Methylene Chloride