title: Development of microsatellite markers in species of Erigeron (Asteraceae) endemic to the Juan Fernández Archipelago, Chile.
abstract: OBJECTIVE
  Microsatellite markers were developed in Erigeron rupicola and tested by amplification in six Erigeron species endemic to the Juan Fernández Archipelago, Chile, to investigate genetic diversity and population structure. •
  
  
  RESULTS
  Using 454 pyrosequencing, 24 primer pairs were developed in E. rupicola, 12 of which amplified and presented polymorphism among endemic species of Erigeron in the Archipelago. Two populations from E. rupicola and E. fernandezianus were genotyped, and one to eight alleles per locus per population were detected. The expected heterozygosity ranged from 0.000 to 0.812. •
  
  
  CONCLUSIONS
  These results indicate the utility of primers for cross-species populational studies in all endemic species of Erigeron in the Archipelago.
authors.affiliation: Department of Systematic and Evolutionary Botany, Biodiversity Center, University of Vienna, Rennweg 14, A-1030 Vienna, Austria. gen33takayama@gmail.com
authors.forename: Koji
authors.initials: K
authors.lastname: Takayama
authors.forename: Patricio
authors.initials: P
authors.lastname: López-Sepúlveda
authors.forename: Gudrun
authors.initials: G
authors.lastname: Kohl
authors.forename: Johannes
authors.initials: J
authors.lastname: Novak
authors.forename: Tod F
authors.initials: TF
authors.lastname: Stuessy
mesh_terms: D002677:Chile; D017931:DNA Primers / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D049919:Erigeron / Q000235:genetics*; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018744:DNA, Plant