title: Novel application of stem cell-derived factors for periodontal regeneration.
abstract: The effect of conditioned medium from cultured mesenchymal stem cells (MSC-CM) on periodontal regeneration was evaluated. In vitro, MSC-CM stimulated migration and proliferation of dog MSCs (dMSCs) and dog periodontal ligament cells (dPDLCs). Cytokines such as insulin-like growth factor, vascular endothelial growth factor, transforming growth factor-β1, and hepatocyte growth factor were detected in MSC-CM. In vivo, one-wall critical-size, intrabony periodontal defects were surgically created in the mandible of dogs. Dogs with these defects were divided into three groups that received MSC-CM, PBS, or no implants. Absorbable atelo-collagen sponges (TERUPLUG®) were used as a scaffold material. Based on radiographic and histological observation 4 weeks after transplantation, the defect sites in the MSC-CM group displayed significantly greater alveolar bone and cementum regeneration than the other groups. These findings suggest that MSC-CM enhanced periodontal regeneration due to multiple cytokines contained in MSC-CM.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Japan. t-inukai@med.nagoya-u.ac.jp
authors.forename: Takeharu
authors.initials: T
authors.lastname: Inukai
authors.forename: Wataru
authors.initials: W
authors.lastname: Katagiri
authors.forename: Ryoko
authors.initials: R
authors.lastname: Yoshimi
authors.forename: Masashi
authors.initials: M
authors.lastname: Osugi
authors.forename: Takamasa
authors.initials: T
authors.lastname: Kawai
authors.forename: Hideharu
authors.initials: H
authors.lastname: Hibi
authors.forename: Minoru
authors.initials: M
authors.lastname: Ueda
mesh_terms: D000539:Alveolar Process / Q000502:physiology; D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000494:pharmacology*; D016207:Cytokines / Q000032:analysis / Q000494:pharmacology*; D004285:Dogs; D008334:Mandible / Q000502:physiology; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D010513:Periodontal Ligament / Q000502:physiology; D010518:Periodontitis / Q000628:therapy*; D010519:Periodontium / Q000187:drug effects* / Q000502:physiology; D012038:Regeneration / Q000187:drug effects; D054457:Tissue Scaffolds
chemical_list: D017077:Culture Media, Conditioned; D016207:Cytokines