title: Optimization of pressurized liquid extraction of three major acetophenones from Cynanchum bungei using a box-behnken design.
abstract: In this work, pressurized liquid extraction (PLE) of three acetophenones  (4-hydroxyacetophenone, baishouwubenzophenone, and 2,4-dihydroxyacetophenone) from Cynanchum bungei (ACB) were investigated. The optimal conditions for extraction of ACB were obtained using a Box-Behnken design, consisting of 17 experimental points, as follows: Ethanol (100%) as the extraction solvent at a temperature of 120 °C and an extraction pressure of 1500 psi, using one extraction cycle with a static extraction time of 17 min. The extracted samples were analyzed by high-performance liquid chromatography using an UV detector. Under this optimal condition, the experimental values agreed  with the predicted values by analysis of variance. The ACB extraction yield with optimal PLE was higher than that obtained by soxhlet extraction and heat-reflux extraction methods. The results suggest that the PLE method provides a good alternative for  acetophenone extraction.
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China. xbgruikang@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Li-Chun
authors.initials: LC
authors.lastname: Zhao
authors.forename: Yin-Shi
authors.initials: YS
authors.lastname: Sun
authors.forename: Feng-Jie
authors.initials: FJ
authors.lastname: Lei
authors.forename: Zi
authors.initials: Z
authors.lastname: Wang
authors.forename: Xiong-Bin
authors.initials: XB
authors.lastname: Gui
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
mesh_terms: D000098:Acetophenones / Q000737:chemistry* / Q000302:isolation & purification*; D000704:Analysis of Variance; D031105:Cynanchum / Q000737:chemistry*; D059625:Liquid-Liquid Extraction / Q000379:methods; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D000098:Acetophenones; D010936:Plant Extracts; C038699:acetophenone