title: Chemical ligation of epoxide-containing fusicoccins and peptide fragments guided by 14-3-3 protein.
abstract: The template effect of 14-3-3 protein on the chemical ligation of fusicoccin derivatives containing an epoxide group and the pentapeptide QSYDC was investigated. HPLC analysis of the epoxide-opening reaction demonstrated that 14-3-3ζ protein improves the yield of the conjugate product compared to the protein-free control.
authors.affiliation: The Institute of Scientific and Industrial Research (ISIR), Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Maki
authors.forename: Akie
authors.initials: A
authors.lastname: Kawamura
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kato
authors.forename: Junko
authors.initials: J
authors.lastname: Ohkanda
mesh_terms: D048948:14-3-3 Proteins / Q000737:chemistry*; D060326:Chemistry Techniques, Synthetic; D002851:Chromatography, High Pressure Liquid; D004852:Epoxy Compounds / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry*; D008956:Models, Chemical; D008958:Models, Molecular; D015394:Molecular Structure; D010446:Peptide Fragments / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D048948:14-3-3 Proteins; D004852:Epoxy Compounds; D006027:Glycosides; D010446:Peptide Fragments; C007808:fusicoccin