title: Using QIIME to analyze 16S rRNA gene sequences from microbial communities.
abstract: QIIME (canonically pronounced "chime") is a software application that performs microbial community analysis. It is an acronym for Quantitative Insights Into Microbial Ecology, and has been used to analyze and interpret nucleic acid sequence data from fungal, viral, bacterial, and archaeal communities. The following protocols describe how to install QIIME on a single computer and use it to analyze microbial 16S sequence data from nine distinct microbial communities.
authors.affiliation: Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado, USA.
authors.forename: Justin
authors.initials: J
authors.lastname: Kuczynski
authors.forename: Jesse
authors.initials: J
authors.lastname: Stombaugh
authors.forename: William Anton
authors.initials: WA
authors.lastname: Walters
authors.forename: Antonio
authors.initials: A
authors.lastname: González
authors.forename: J Gregory
authors.initials: JG
authors.lastname: Caporaso
authors.forename: Rob
authors.initials: R
authors.lastname: Knight
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D044822:Biodiversity; D019295:Computational Biology / Q000295:instrumentation / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*; D012984:Software
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S