title: [Meiotic analysis of spermatogenic cells in severe oligoasthenoteratozoospermia with chromosome 13 rearrangement].
abstract: OBJECTIVE
  To explore the possible mechanisms of spermatogenic arrest in severe oligoasthenoteratozoospermia induced by supernumerary, ring-neocentric 13q12.3 --> 13q22 chromosome and reciprocal deletion.
  
  
  METHODS
  We performed a genomic-wide high-density oaCGH analysis for a case of oligoasthenoteratozoospermia with abnormal chromosome 13 to characterize the breakpoints of the chromosome involved or the gene deletion caused by the rearrangement. We also conducted a fluorescence in situ hybridization analysis on the germ cells using probes of 13q14/13qter to observe the pairing condition of homologous chromosome 13.
  
  
  RESULTS
  We identified by oaCGH analysis a microdeletion of 4 consecutive probes (A_16_P19757882, A_16_P02744617, A_14_ P108858 and A_16_P02744687 at chr13q12.3: 27979261 --> 28039191) with 59.93 kb between the FLT1 and POMP genes, with no annotated genes in the deleted region. The signals of 13q14 and 13qter were separated from each other in 90% of all the primary spermatocytes examined, indicating the unpairing of homologous chromosome 13 or synapse failure.
  
  
  CONCLUSIONS
  Chromosomal rearrangement-induced spermatogenesis failure is caused by the unpairing of the homologous chromosomes involved in the first meiotic division of germ cells.
authors.affiliation: Department of Central Laboratory, PLA Research Institute of Clinical Laboratory Medicine, Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China. cuiyix55@yahoo.com.cn
authors.forename: Ying-Xia
authors.initials: YX
authors.lastname: Cui
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Yang
authors.forename: Xin-Yi
authors.initials: XY
authors.lastname: Xia
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Ying-Hao
authors.initials: YH
authors.lastname: Chen
authors.forename: Yu-An
authors.initials: YA
authors.lastname: Hu
mesh_terms: D000328:Adult; D053627:Asthenozoospermia / Q000235:genetics*; D053713:Azoospermia / Q000151:congenital; D002869:Chromosome Aberrations; D056905:Chromosome Breakpoints; D002882:Chromosomes, Human, Pair 13; D055028:Comparative Genomic Hybridization; D006801:Humans; D008297:Male; D008540:Meiosis; D009845:Oligospermia / Q000235:genetics*; D013091:Spermatogenesis / Q000235:genetics