title: The expression and role of Lysyl oxidase (LOX) in dentinogenesis.
abstract: OBJECTIVE
  To establish whether eliminating Lysyl oxidase (LOX) gene would affect dentine formation.
  
  
  METHODS
  Newborn wild-type (wt) and homo- and heterozygous LOX knock-out (Lox(-/-) and Lox(+/-) , respectively) mice were used to study developing tooth morphology and dentine formation. Collagen aggregation in the developing dentine was examined histochemically with picrosirius red (PSR) staining followed by polarized microscopy. Because Lox(-/-) die at birth, adult wt and Lox(+/-) mouse tooth morphologies were examined with FESEM. Human odontoblasts and pulp tissue were used to study the expression of LOX and its isoenzymes with Affymetrix cDNA microarray.
  
  
  RESULTS
  No differences between Lox(-/-) , Lox(+/-) and wt mice developing tooth morphology were seen by light microscopy. Histochemically, however, teeth in wt mice demonstrated yellow-orange and orange-red polarization colours with PSR staining, indicating thick and more densely packed collagen fibres, whilst in Lox(-/-) and Lox(+/-) mice, most of the polarization colours were green to green-yellow, indicating thinner, less aggregated collagen fibres. Fully developed teeth did not show any differences between Lox(+/-) and wt mice with FESEM. Human odontoblasts expressed LOX and three of four of its isoenzymes.
  
  
  CONCLUSIONS
  The data indicate that LOX is not essential in dentinogenesis, even though LOX deletion may affect dentine matrix collagen thickness and packing. The absence of functional LOX may be compensated by LOX isoenzymes.
authors.affiliation: Institute of Dentistry, University of Oulu, Oulu, Finland; Oulu University Hospital, Oulu, Finland. leo.Tja¨derhane@oulu.fi
authors.forename: L
authors.initials: L
authors.lastname: Tjäderhane
authors.forename: M
authors.initials: M
authors.lastname: Vered
authors.forename: V
authors.initials: V
authors.lastname: Pääkkönen
authors.forename: A
authors.initials: A
authors.lastname: Peteri
authors.forename: J M
authors.initials: JM
authors.lastname: Mäki
authors.forename: J
authors.initials: J
authors.lastname: Myllyharju
authors.forename: D
authors.initials: D
authors.lastname: Dayan
authors.forename: T
authors.initials: T
authors.lastname: Salo
mesh_terms: D000566:Amelogenesis / Q000235:genetics / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D001391:Azo Compounds; D003094:Collagen / Q000648:ultrastructure; D004396:Coloring Agents; D003782:Dental Pulp / Q000201:enzymology; D003804:Dentin / Q000648:ultrastructure; D003810:Dentinogenesis / Q000235:genetics / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007527:Isoenzymes / Q000032:analysis / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D008855:Microscopy, Electron, Scanning; D008859:Microscopy, Polarization; D009804:Odontoblasts / Q000201:enzymology; D009805:Odontogenesis / Q000235:genetics / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D008249:Protein-Lysine 6-Oxidase / Q000032:analysis* / Q000235:genetics / Q000502:physiology
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D016326:Extracellular Matrix Proteins; D007527:Isoenzymes; C009798:C.I. direct red 80; C077112:Lox protein, mouse; D003094:Collagen; D008249:Protein-Lysine 6-Oxidase