title: Time-gated optical projection tomography allows visualization of adult zebrafish internal structures.
abstract: Optical imaging through biological samples is compromised by tissue scattering and currently various approaches aim to overcome this limitation. In this paper we demonstrate that an all optical technique, based on non-linear upconversion of infrared ultrashort laser pulses and on multiple view acquisition, allows the reduction of scattering effects in tomographic imaging. This technique, namely Time-Gated Optical Projection Tomography (TGOPT), is used to reconstruct three dimensionally the internal structure of adult zebrafish without staining or clearing agents. This method extends the use of Optical Projection Tomography to optically diffusive samples yielding reconstructions with reduced artifacts, increased contrast and improved resolution with respect to those obtained with non-gated techniques. The paper shows that TGOPT is particularly suited for imaging the skeletal system and nervous structures of adult zebrafish.
authors.affiliation: Department of Physics, Politecnico di Milano, Milano, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Fieramonti
authors.forename: Andrea
authors.initials: A
authors.lastname: Bassi
authors.forename: Efrem Alessandro
authors.initials: EA
authors.lastname: Foglia
authors.forename: Anna
authors.initials: A
authors.lastname: Pistocchi
authors.forename: Cosimo
authors.initials: C
authors.lastname: D'Andrea
authors.forename: Gianluca
authors.initials: G
authors.lastname: Valentini
authors.forename: Rinaldo
authors.initials: R
authors.lastname: Cubeddu
authors.forename: Sandro
authors.initials: S
authors.lastname: De Silvestri
authors.forename: Giulio
authors.initials: G
authors.lastname: Cerullo
authors.forename: Franco
authors.initials: F
authors.lastname: Cotelli
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000033:anatomy & histology*; D016477:Artifacts; D001842:Bone and Bones / Q000033:anatomy & histology*; D021621:Imaging, Three-Dimensional; D007834:Lasers; D008027:Light; D009420:Nervous System / Q000033:anatomy & histology*; D013997:Time Factors; D041622:Tomography, Optical / Q000295:instrumentation / Q000379:methods*; D015027:Zebrafish / Q000033:anatomy & histology* / Q000235:genetics