title: Effects of medium supplements on proliferation, differentiation potential, and in vitro expansion of mesenchymal stem cells.
abstract: Mesenchymal stem cells (MSCs) possess great potential for use in regenerative medicine. However, their clinical application may be limited by the ability to expand their cell numbers in vitro while maintaining their differential potentials and stem cell properties. Thus the aim of this study was to test the effect of a range of medium supplements on MSC self-renewal and differentiation potential. Cells were cultured until confluent and subcultured continuously until reaching senescence. Medium supplementation with fibroblast growth factor (FGF)-2, platelet-derived growth factor (PDGF)-BB, ascorbic acid (AA), and epidermal growth factor (EGF) both increased proliferation rate and markedly increased number of cell doublings before reaching senescence, with a greater than 1,000-fold increase in total cell numbers for AA, FGF-2, and PDGF-BB compared with control cultures. Long-term culture was associated with loss of osteogenic/adipocytic differentiation potential, particularly with FGF-2 supplementation but also with AA, EGF, and PDGF-BB. In addition FGF-2 resulted in reduction in expression of CD146 and alkaline phosphatase, but this was partially reversible on removal of the supplement. Cells expressed surface markers including CD146, CD105, CD44, CD90, and CD71 by flow cytometry throughout, and expression of these putative stem cell markers persisted even after loss of differentiation potentials. Overall, medium supplementation with FGF-2, AA, EGF, and PDGF-BB greatly enhanced the total in vitro expansion capacity of MSC cultures, although differentiation potentials were lost prior to reaching senescence. Loss of differentiation potential was not reflected by changes in stem cell surface marker expression.
authors.affiliation: Department of Periodontology, King's College London, London, UK.
authors.forename: Borzo
authors.initials: B
authors.lastname: Gharibi
authors.forename: Francis J
authors.initials: FJ
authors.lastname: Hughes
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism; D001205:Ascorbic Acid / Q000494:pharmacology; D000077214:Becaplermin; D051929:CD146 Antigen / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence; D003470:Culture Media; D004260:DNA Repair; D000071063:Endoglin; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D020574:Proto-Oncogene Proteins c-sis / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000378:metabolism; D011990:Receptors, Transferrin / Q000378:metabolism; D018800:Thy-1 Antigens / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D051929:CD146 Antigen; C068126:CD71 antigen; D003470:Culture Media; C064206:ENG protein, human; D000071063:Endoglin; D018960:Hyaluronan Receptors; D020574:Proto-Oncogene Proteins c-sis; D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin; D018800:Thy-1 Antigens; D016222:Fibroblast Growth Factor 2; D000077214:Becaplermin; D004815:Epidermal Growth Factor; D000469:Alkaline Phosphatase; D001205:Ascorbic Acid