title: Radix Paeoniae Rubra and Radix Paeoniae Alba Attenuate CCl4-induced acute liver injury: an ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) based metabolomic approach for the pharmacodynamic study of Traditional Chinese Medicines (TCMs).
abstract: Metabolomics has been frequently used in pharmacodynamic studies, especially those on traditional Chinese medicine (TCM). Radix Paeoniae Alba and Radix Paeoniae Rubra are popularly used in TCM, and both have hepatoprotective effects. In this study, a CCl(4)-induced acute liver injury rat model was established and confirmed by the observed serum aminotransferase activities. The metabolomics approach was applied to study the influence of Radix Paeoniae Alba and Radix Paeoniae Rubra on the metabolic changes in rats with acute liver injury. The partial least-squares-discriminant analysis (PLS-DA) of rat serum and their ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) fingerprints allowed discrimination of controlled, acute liver injury-model rats after administration of the two types of TCMs. The time-dependent PLS-DA plots showed that the changes in the metabolic patterns of the rats, which were administered with the TCMs, had stabilized within 2 h after they received the intraperitoneal CCl(4) injection. The results indicated the protective effect of TCMs against liver injury. Several potential biomarkers were detected and identified, which included creatine, deoxycholic acid, choline, 5-methylenetetrahydrofolate, folic acid, and glycocholic acid. The physiological significance of these metabolic changes was discussed.
authors.affiliation: School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. cpuyl@126.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Ai-Zhen
authors.initials: AZ
authors.lastname: Xiong
authors.forename: Zhong-Qiu
authors.initials: ZQ
authors.lastname: Teng
authors.forename: Qi-Wei
authors.initials: QW
authors.lastname: Yang
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Shi
authors.forename: Li
authors.initials: L
authors.lastname: Yang
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D002251:Carbon Tetrachloride / Q000009:adverse effects; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism*; D002853:Chromatography, Liquid; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D008111:Liver Function Tests; D008297:Male; D013058:Mass Spectrometry; D008516:Medicine, Chinese Traditional; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D029596:Paeonia / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology*; D020011:Protective Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D051381:Rats
chemical_list: D001647:Bile Acids and Salts; D015415:Biomarkers; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D020011:Protective Agents; D002251:Carbon Tetrachloride