title: A novel function for SNAP29 (synaptosomal-associated protein of 29 kDa) in mast cell phagocytosis.
abstract: Mast cells play a critical role in the innate immune response to bacterial infection. They internalize and kill a variety of bacteria and process antigen for presentation to T cells via MHC molecules. Although mast cell phagocytosis appears to play a significant role during bacterial infection, little is known about the proteins involved in its regulation. In this study, we demonstrate that the SNARE protein SNAP29 is involved in mast cell phagocytosis. SNAP29 is localized in the endocytic pathway and is transiently recruited to Escherichia coli (E. coli)-containing phagosomes. Interestingly, overexpression of SNAP29 significantly increases the internalization and killing of E. coli, while it does not affect mast cell exocytosis of inflammatory mediators. To our knowledge, these data are the first to demonstrate a novel function of SNAP29 in mast cell phagocytosis and have implications in protection against bacterial infection.
authors.affiliation: Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America.
authors.forename: Jordan
authors.initials: J
authors.lastname: Wesolowski
authors.forename: Vernon
authors.initials: V
authors.lastname: Caldwell
authors.forename: Fabienne
authors.initials: F
authors.lastname: Paumet
mesh_terms: D018830:Adhesins, Escherichia coli / Q000276:immunology; D000818:Animals; D001424:Bacterial Infections / Q000276:immunology / Q000378:metabolism; D004926:Escherichia coli; D006801:Humans; D007113:Immunity, Innate; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism; D010587:Phagocytosis / Q000276:immunology / Q000502:physiology; D010588:Phagosomes / Q000235:genetics / Q000378:metabolism; D050766:Qb-SNARE Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D050767:Qc-SNARE Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism
chemical_list: D018830:Adhesins, Escherichia coli; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; C117418:Snap29 protein, rat