title: An electrochemical biosensor based on human serum albumin/graphene oxide/3-aminopropyltriethoxysilane modified ITO electrode for the enantioselective discrimination of D- and L-tryptophan.
abstract: A new electrochemical biosensor based on the human serum albumin/graphene oxide/3-aminopropyl-triethoxysilane modified indium tin oxide electrode (ITO/APTES/GO/HSA) has been developed for the discrimination of tryptophan (Trp) enantiomers.The electrode has been characterized by scanning electron microscopy (SEM) and electrochemical techniques. The electrochemical behaviors of the enantiomeric pairs (D- and L-Trp) at the ITO/APTES/GO/HSA electrode have been investigated by cyclic voltammetry in the concentration range of 0.10-1.0 mM. A clear separation between the oxidation peak potentials of D- and L-Trp, at 0.86 and 1.26 V, respectively, has suggested that the ITO/APTES/GO/HSA electrode can be used as an electrochemical biosensor for the discrimination of Trp enantiomers. In order to find the percentage of an enantiomeric form of tryptophan in a mixture, the ITO/APTES/GO/HSA electrode is used for the simultaneous detection of D- and L-Trp which showed that the percentage of one enantiomeric form can be easily measured in the presence of the other.
authors.affiliation: Science Institute, Selcuk University, 42003 Selcuklu/Konya, Turkey. zorerhan@gmail.com
authors.forename: Erhan
authors.initials: E
authors.lastname: Zor
authors.forename: Imren
authors.initials: I
authors.lastname: Hatay Patir
authors.forename: Haluk
authors.initials: H
authors.lastname: Bingol
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ersoz
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D011437:Propylamines; D012709:Serum Albumin / Q000737:chemistry; D012821:Silanes / Q000737:chemistry; D017971:Tin Compounds / Q000737:chemistry; D014364:Tryptophan / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D011437:Propylamines; D012709:Serum Albumin; D012821:Silanes; D017971:Tin Compounds; C109984:indium tin oxide; D006108:Graphite; D014364:Tryptophan; C477625:amino-propyl-triethoxysilane