title: Production of cellulolytic enzymes by Aspergillus fumigatus ABK9 in wheat bran-rice straw mixed substrate and use of cocktail enzymes for deinking of waste office paper pulp.
abstract: Response surface methodology was employed to optimize mixed substrate solid state fermentation for the production of cellulases and xylanase by Aspergillus fumigatus ABK9. Among 11 different parameters, fermentation time (86-88 h), medium pH (6.1-6.2), substrate amount (10.0-10.5 g) and substrate ratio (wheat bran:rice straw) (1.1) had significantly influences on enzyme production. Under these conditions endoglucanase, β-glucosidase, FPase (filter paper degrading activity) and xylanase activities of 826.2, 255.16, 102.5 and 1130.4 U/g, respectively were obtained. The enzyme cocktail extracted (solid to water ratio of 1:10) from the ferments increased brightness of waste office paper pulp by 82.8% ISO, Ink(D) value by 82.1%, removed chromophores (2.53 OD; A(237)nm) and hydrophobic compounds (1.15 OD; A(465)nm) and also decreased the kappa number to 13.5 from 16.8.
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Arpan
authors.initials: A
authors.lastname: Das
authors.forename: Tanmay
authors.initials: T
authors.lastname: Paul
authors.forename: Suman K
authors.initials: SK
authors.lastname: Halder
authors.forename: Arijit
authors.initials: A
authors.lastname: Jana
authors.forename: Chiranjit
authors.initials: C
authors.lastname: Maity
authors.forename: Pradeep K
authors.initials: PK
authors.lastname: Das Mohapatra
authors.forename: Bikas R
authors.initials: BR
authors.lastname: Pati
authors.forename: Keshab C
authors.initials: KC
authors.lastname: Mondal
mesh_terms: D001232:Aspergillus fumigatus / Q000201:enzymology*; D002480:Cellulase / Q000096:biosynthesis* / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry; D007220:Industrial Waste / Q000517:prevention & control; D007281:Ink; D012275:Oryza / Q000382:microbiology*; D010209:Paper; D014908:Triticum / Q000382:microbiology*
chemical_list: D007220:Industrial Waste; D002482:Cellulose; D002480:Cellulase