title: Simple multiresidue extraction method for the determination of fungicides and plant growth regulator in bean sprouts using low temperature partitioning and tandem mass spectrometry.
abstract: A simple multiresidue analytical method is developed for the simultaneous determination of carbendazim (CB), thiabendazole (TB), and 6-benzyl aminopurine (6-BA) in bean sprouts. The samples were extracted with acetonitrile followed by partitioning at -80°C for 5-10 min. A YMC C(8) column was used to separate the analytes before being qualitatively and quantitatively determined by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) in positive ion mode using multiple reaction monitoring (MRM). The matrix-matched calibration curves showed good linearity in the range 0.01-1.0 mg/kg with correlation coefficients in excess of 0.998. The mean recoveries were in the range of 80.4-96.3% at 0.1 and 0.5 spiked levels, and the relative standard deviations (RSDs) were in the range of 0.5-7.6%. The limits of quantifications (LOQ) were in the range of 0.005-0.01 mg/kg. The method was successfully applied to 90 samples (among which 45 were organic) collected from a commercial bean sprout production house throughout the city. Except for 6-BA, the rest of the analytes had values lower than their LOQs. In sum, carbendazim, thiabendazole, and 6-BA were extracted in a single step, and no steps for clean-up or concentration of the extracts were needed. The current method can be used for sensitive and accurate determination and confirmation of residues in bean sprout samples.
authors.affiliation: National Agricultural Products Quality Management Service, Products Safety Inspection Laboratory, Gwangsan-Gu, Gwangju, Republic of Korea.
authors.forename: Soon-Kil
authors.initials: SK
authors.lastname: Cho
authors.forename: A M
authors.initials: AM
authors.lastname: Abd El-Aty
authors.forename: Ki Hun
authors.initials: KH
authors.lastname: Park
authors.forename: Jong-Hyouk
authors.initials: JH
authors.lastname: Park
authors.forename: M E
authors.initials: ME
authors.lastname: Assayed
authors.forename: Yang-Mo
authors.initials: YM
authors.lastname: Jeong
authors.forename: Young-Seok
authors.initials: YS
authors.lastname: Park
authors.forename: Jae-Han
authors.initials: JH
authors.lastname: Shim
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid; D007887:Fabaceae / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis; D005659:Fungicides, Industrial / Q000032:analysis* / Q000302:isolation & purification*; D010573:Pesticide Residues / Q000032:analysis* / Q000302:isolation & purification*; D010937:Plant Growth Regulators / Q000032:analysis* / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry; D014675:Vegetables / Q000737:chemistry
chemical_list: D005659:Fungicides, Industrial; D010573:Pesticide Residues; D010937:Plant Growth Regulators