title: Plant Regeneration from Pea Leaflets Cultured in vitro and Genetic Stability of Regenerants.
abstract: Bud and shoot formation and regeneration of complete plants have been achieved with immature and mature leaflets, at various frequency, under the influence of a range of cytokinin and auxin combinations. Immature leaflets, 0.9 to 1.8 mm in length, produced shoots in 26 to 38% explants on a medium containing 10.0 μM BA and NAA, IBA or IAA. A reduction in the frequency of bud and shoot regeneration to 7% in the presence of 10.0 μM each of BA + NAA was observed with mature leaflets. In the differentiation of buds and shoots, no stimulating effect of GA(3) has been observed. Combinations of cytokinins with picloram and 2,4-D suppressed organogenesis. Plant regeneration was influenced by genotype and temperatures. Seed-derived progeny of regenerants contained the normal diploid number of chromosomes (2n = 14). Analysis of four isozymes, esterase, GDH, 6-PGD, and LAP, as well as nine genetically defined morphological characters indicated retention of genetic stability in the progeny of tisssue culture propagules.
authors.affiliation: Plant Biotechnology Institure, National Research Council, Saskatoon, Saskatchewan, Canada S7N 0W9.
authors.forename: A
authors.initials: A
authors.lastname: Rubluo
authors.forename: K K
authors.initials: KK
authors.lastname: Kartha
authors.forename: L A
authors.initials: LA
authors.lastname: Mroginski
authors.forename: J
authors.initials: J
authors.lastname: Dyck