title: Characterization of a novel α-conotoxin from conus textile that selectively targets α6/α3β2β3 nicotinic acetylcholine receptors.
abstract: α6β2 Nicotinic acetylcholine receptors (nAChRs) expressed by dopaminergic neurons in the CNS are potential therapeutic targets for the treatment of several neuropsychiatric diseases, including nicotine addiction and Parkinson disease. However, recent studies indicate that the α6 subunit can also associate with the β4 subunit to form α6β4 nAChRs that are difficult to pharmacologically distinguish from α6β2, α3β4, and α3β2 subtypes. The current study characterized a novel 16-amino acid α-conotoxin (α-CTx) TxIB from Conus textile whose sequence is GCCSDPPCRNKHPDLC-amide as deduced from gene cloning. The peptide and an analog with an additional C-terminal glycine were chemically synthesized and tested on rat nAChRs heterologously expressed in Xenopus laevis oocytes. α-CTx TxIB blocked α6/α3β2β3 nAChR with an IC(50) of 28 nm. In contrast, the peptide showed little or no block of other tested subtypes at concentrations up to 10 μm. The three-dimensional solution structure of α-CTx TxIB was determined using NMR spectroscopy. α-CTx TxIB represents a uniquely selective ligand for probing the structure and function of α6β2 nAChRs.
authors.affiliation: Key Laboratory of Tropical Biological Resources, Ministry of Education, Key Lab for Marine Drug of Haikou, Hainan University, Haikou Hainan, 570228 China. luosulan2003@163.com
authors.forename: Sulan
authors.initials: S
authors.lastname: Luo
authors.forename: Dongting
authors.initials: D
authors.lastname: Zhangsun
authors.forename: Yong
authors.initials: Y
authors.lastname: Wu
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Zhu
authors.forename: Yuanyan
authors.initials: Y
authors.lastname: Hu
authors.forename: Melissa
authors.initials: M
authors.lastname: McIntyre
authors.forename: Sean
authors.initials: S
authors.lastname: Christensen
authors.forename: Muharrem
authors.initials: M
authors.lastname: Akcan
authors.forename: David J
authors.initials: DJ
authors.lastname: Craik
authors.forename: J Michael
authors.initials: JM
authors.lastname: McIntosh
mesh_terms: D000818:Animals; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D020916:Conotoxins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology* / Q000633:toxicity; D052078:Conus Snail / Q000737:chemistry*; D017931:DNA Primers; D008968:Molecular Conformation; D019906:Nuclear Magnetic Resonance, Biomolecular; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D011978:Receptors, Nicotinic / Q000187:drug effects*
chemical_list: D020916:Conotoxins; C059360:Conus textile toxin; D017931:DNA Primers; D011978:Receptors, Nicotinic