title: Succinic acid production from cellobiose by Actinobacillus succinogenes.
abstract: In this study, cellobiose, a reducing disaccharide was used to produce succinic acid by Actinobacillus succinogenes NJ113. A final succinic acid concentration of 30.3g/l with a yield of 67.8% was achieved from an initial cellobiose concentration of 50 g/l via batch fermentation in anaerobic bottles. The cellobiose uptake mechanism was investigated and the results of enzyme assays revealed that the phosphoenolpyruvate phosphotransferase system (PEP-PTS) played an important role in the cellobiose uptake process. In batch fermentation with 18 g/l of cellobiose and 17 g/l of other sugars from sugarcane bagasse cellulose hydrolysates, a succinic acid concentration of 20.0 g/l was obtained, with a corresponding yield of 64.7%. This study found that cellobiose from incomplete hydrolysis of cellulose could be a potential carbon source for economical and efficient succinic acid production by A. succinogenes.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Puzhu South Road 30#, Nanjing 211816, PR China. bioengine@njut.edu.cn
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Rong
authors.initials: R
authors.lastname: Xu
authors.forename: Yong-Lan
authors.initials: YL
authors.lastname: Xi
authors.forename: Jiu-Hua
authors.initials: JH
authors.lastname: Zhang
authors.forename: Wen-Yu
authors.initials: WY
authors.lastname: Dai
authors.forename: Yue-Jia
authors.initials: YJ
authors.lastname: Wan
authors.forename: Ke-Quan
authors.initials: KQ
authors.lastname: Chen
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
mesh_terms: D000188:Actinobacillus / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D019149:Bioreactors / Q000382:microbiology; D002241:Carbohydrates / Q000494:pharmacology; D002244:Carbon / Q000494:pharmacology; D002475:Cellobiose / Q000378:metabolism* / Q000494:pharmacology; D002482:Cellulose / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism; D019802:Succinic Acid / Q000378:metabolism*; D013997:Time Factors
chemical_list: D002241:Carbohydrates; D002475:Cellobiose; D002244:Carbon; D002482:Cellulose; C027433:bagasse; D019802:Succinic Acid; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System