title: Recruitment and rearrangement of three different genetic determinants into a conjugative plasmid increase copper resistance in Pseudomonas syringae.
abstract: We describe the genetic organization of a copper-resistant plasmid containing copG and cusCBA genes in the plant pathogen Pseudomonas syringae. Chromosomal variants of czcCBA and a plasmid variant of cusCBA were present in different P. syringae pathovar strains. Transformation of the copper-sensitive Pseudomonas syringae pv. syringae FF5 strain with copG or cusCBA conferred copper resistance, and quantitative real-time PCR (qRT-PCR) experiments confirmed their induction by copper.
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora (IHSM-UMA-CSIC), Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Málaga, Spain.
authors.forename: José A
authors.initials: JA
authors.lastname: Gutiérrez-Barranquero
authors.forename: Antonio
authors.initials: A
authors.lastname: de Vicente
authors.forename: Víctor J
authors.initials: VJ
authors.lastname: Carrión
authors.forename: George W
authors.initials: GW
authors.lastname: Sundin
authors.forename: Francisco M
authors.initials: FM
authors.lastname: Cazorla
mesh_terms: D003227:Conjugation, Genetic; D003300:Copper / Q000378:metabolism / Q000633:toxicity*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D023061:Gene Order; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D044224:Pseudomonas syringae / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014169:Transformation, Bacterial
chemical_list: D004269:DNA, Bacterial; D003300:Copper