title: Optimization of lipase production by Burkholderia sp. using response surface methodology.
abstract: Response surface methodology (RSM) was employed to optimize the extracellular lipase production by Burkholderia sp. HL-10. Preliminary tests showed that olive oil, tryptone and Tween-80 exhibited significant effects on the lipase production. The optimum concentrations of these three components were determined using a faced-centered central composite design (FCCCD). The analysis of variance revealed that the established model was significant (p < 0.01). The optimized medium containing 0.65% olive oil (v/v), 2.42% tryptone (w/v) and 0.15% Tween-80 (v/v) resulted in a maximum activity of 122.3 U/mL, about three fold higher than that in basal medium. Approximately 99% of validity of the predicted value was achieved.
authors.affiliation: Department of Bioengineering, Tatung University, No 40, Sec. 3, Zhongshan N. Rd., Taipei 104, Taiwan. slee@ttu.edu.tw
authors.forename: Chia-Feng
authors.initials: CF
authors.lastname: Lo
authors.forename: Chi-Yang
authors.initials: CY
authors.lastname: Yu
authors.forename: I-Ching
authors.initials: IC
authors.lastname: Kuan
authors.forename: Shiow-Ling
authors.initials: SL
authors.lastname: Lee
mesh_terms: D000704:Analysis of Variance; D019117:Burkholderia / Q000187:drug effects / Q000378:metabolism*; D003470:Culture Media; D004789:Enzyme Activation / Q000187:drug effects; D005110:Extracellular Space / Q000378:metabolism; D008049:Lipase / Q000096:biosynthesis*; D000069463:Olive Oil; D010461:Peptones / Q000494:pharmacology; D010938:Plant Oils / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology
chemical_list: D003470:Culture Media; D000069463:Olive Oil; D010461:Peptones; D010938:Plant Oils; D011136:Polysorbates; C001830:tryptones; D008049:Lipase