title: Culture conditions and medium components for the production of mycelial biomass and exo-polysaccharides with Paecilomyces japonica in liquid culture.
abstract: In this study, the liquid culture conditions were optimized for maximal production of mycelial biomass and exo-polysaccharide by Paecilomyces japonica. The effects of medium composition, C/N ratio and physical parameters were investigated. From these experiments, 30 g glucose, 20 g yeast extract, 0.5 g KH2PO4, and 0.1 g CuCl2 2H2O in 1-l distilled water were found to be the most suitable carbon, nitrogen, and mineral sources, respectively. The optimal temperature, initial pH, agitation, and aeration were determined to be 27°C, uncontrolled pH, 400 rpm, and 1.0 vvm, respectively. Under these optimal conditions, the maximum mycelial growth and polysaccharides production were 23.1 g/l and 2.5 g/l, respectively.
authors.affiliation: Department of Bioengineering and Technology, Kangwon National University, Kangwon-do, Republic of Korea.
authors.forename: Jong Seok
authors.initials: JS
authors.lastname: Lee
authors.forename: Woo Chul
authors.initials: WC
authors.lastname: Jung
authors.forename: Seok Jae
authors.initials: SJ
authors.lastname: Park
authors.forename: Keun Eok
authors.initials: KE
authors.lastname: Lee
authors.forename: Won Cheol
authors.initials: WC
authors.lastname: Shin
authors.forename: Eock Kee
authors.initials: EK
authors.lastname: Hong
mesh_terms: D018533:Biomass; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D025282:Mycelium / Q000254:growth & development*; D009584:Nitrogen / Q000378:metabolism; D010143:Paecilomyces / Q000254:growth & development / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry; D013696:Temperature; D014131:Trace Elements / Q000378:metabolism
chemical_list: D003470:Culture Media; D011135:Polysaccharides, Bacterial; D014131:Trace Elements; D002244:Carbon; D009584:Nitrogen