title: Oral vaccination of BacFish-vp6 against grass carp reovirus evoking antibody response in grass carp.
abstract: To obtain a vaccine and evaluate its immune efficacy as an oral vaccine against Grass Carp Reovirus (GCRV), a donor vector pFastBac-FA-VP6-ph-VP6 containing two GCRV vp6 genes, one driven by the Megalobrama amblycephala β-actin promoter and another one controlled by baculovirus polyhedrin promoter was constructed to generate the recombinant baculovirus BacFish-vp6. From the hemolymph of 5th instar silkworm inoculated with BacFish-vp6, a 53 kDa recombinant VP6 protein could be detected. And the infected pupae collected at 120 h post-inoculation with BacFish-vp6 were used to make freeze-dried powder as an oral vaccine. When the grass carps were orally administrated with feed containing 1%, 5% and 10% of the freeze-dried powder, their specific antibody against VP6 could be detected. Further studies showed that vp6 has been transcripted in the grass carp kidney (CIK) cells infected with BacFish-vp6 and the different tissues of orally vaccinated fish. All the results suggested the powder of the silkworm pupae infected with BacFish-vp6 could possibly be used as an orally administered vaccine.
authors.affiliation: School of Biology & Basic Medical Sciences, Soochow University, Suzhou 215123, China.
authors.forename: Renyu
authors.initials: R
authors.lastname: Xue
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Guangli
authors.initials: G
authors.lastname: Cao
authors.forename: Shiying
authors.initials: S
authors.lastname: Xu
authors.forename: Jinghui
authors.initials: J
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Zou
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Chengliang
authors.initials: C
authors.lastname: Gong
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D012831:Bombyx; D002347:Carps; D005393:Fish Diseases / Q000276:immunology*; D007814:Larva; D012087:Reoviridae / Q000502:physiology; D012088:Reoviridae Infections / Q000276:immunology / Q000662:veterinary*; D014611:Vaccination / Q000662:veterinary
chemical_list: D000914:Antibodies, Viral