title: Prevalence of Providencia strains among children with diarrhea in Japan.
abstract: In the present study, we examined the prevalence of Providencia spp. strains among children with diarrhea in Japan. We developed a Providencia genus-specific polymerase chain reaction (PCR) method, and the specificity and sensitivity was evaluated to be 100% with various bacterial strains including 7 genera and 13 species. Five of 345 samples (1.4%) were positive by PCR using a Providencia genus-specific primer targeting the 16S rRNA gene. The single species Providencia rettgeri was isolated from 4 stool samples of children with diarrhea. The prevalence of Providencia spp. in children with diarrhea in Japan is lower than that previously reported for Japanese travelers abroad with diarrhea.
authors.affiliation: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Shima
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hinenoya
authors.forename: Masahiro
authors.initials: M
authors.lastname: Asakura
authors.forename: Akira
authors.initials: A
authors.lastname: Nagita
authors.forename: Shinji
authors.initials: S
authors.lastname: Yamasaki
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods; D002648:Child; D002675:Child, Preschool; D017931:DNA Primers / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D003967:Diarrhea / Q000453:epidemiology* / Q000382:microbiology*; D004756:Enterobacteriaceae Infections / Q000453:epidemiology* / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007564:Japan / Q000453:epidemiology; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D011532:Providencia / Q000145:classification / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S