title: Expression and clinical significance of FAK, ILK, and PTEN in salivary adenoid cystic carcinoma.
abstract: CONCLUSIONS
  Focal adhesion kinase (FAK) and integrin-linked kinase (ILK) are highly expressed in salivary adenoid cystic carcinoma (SACC), especially metastatic SACC, suggesting their potential role as prognostic markers for SACC.
  
  
  OBJECTIVE
  This study aimed to investigate the expression of FAK, ILK, and phosphatase and tensin homolog deleted on chromosome ten (PTEN) in SACC tissues and the clinical significance.
  
  
  METHODS
  PAK and ILK expression in samples from 50 cases of SACC and 12 subjects with normal salivary glands was detected by immunohistochemical analysis. PAK and ILK expression in SACC cell lines was detected by RT-PCR and Western blot analysis.
  
  
  RESULTS
  The positive rate of PAK and ILK staining was 94% (47/50) and 48% (24/50) in SACC, respectively, significantly higher than in normal salivary gland tissues (p < 0.05). However, the positive rate of PTEN staining was 30% (15/50), significantly lower than in normal salivary gland tissues (p < 0.05). Pearson analysis showed that FAK expression was positively correlated with ILK expression but negatively correlated with PTEN expression in SACC tissues. FAK and ILK expression was positively associated with advanced stage, solid histological subtype, perineural invasion, and distant metastasis of SACC (p < 0.05). In addition, FAK and ILK expression at both mRNA and protein levels was significantly higher in highly metastatic SACC-LM cells than low-metastatic SACC-83 cells.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital, Liaoning Medical University, Jinzhou, PR China. entdoctorchen@163.com
authors.forename: Dong
authors.initials: D
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Kang
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ma
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.forename: Liang
authors.initials: L
authors.lastname: Gong
mesh_terms: D000328:Adult; D000368:Aged; D015153:Blotting, Western; D003528:Carcinoma, Adenoid Cystic / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D051417:Focal Adhesion Kinase 1 / Q000096:biosynthesis / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D051059:PTEN Phosphohydrolase / Q000096:biosynthesis / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics*; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012468:Salivary Gland Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D012469:Salivary Glands / Q000378:metabolism / Q000473:pathology; D025521:Tumor Suppressor Proteins; D055815:Young Adult; D000099200:Scaffold Protein ILK
chemical_list: D051417:Focal Adhesion Kinase 1; D051059:PTEN Phosphohydrolase; D017346:Protein Serine-Threonine Kinases; D012334:RNA, Neoplasm; D025521:Tumor Suppressor Proteins; D000099200:Scaffold Protein ILK; C496050:PTK2 protein, human; C494929:PTEN protein, human