title: In vitro evaluation of gene expression changes for gonadotropin-releasing hormone 1, brain-derived neurotrophic factor and neurotrophic tyrosine kinase, receptor, type 2, in response to bisphenol A treatment.
abstract: We evaluated the effects of bisphenol A (BPA) on embryonic mouse hypothalamic cells. Real-time reverse transcription polymerase chain reaction (RT-PCR) indicated that gonadotropin-releasing hormone 1 (Gnrh1) expression in 0.02-20 μM BPA-treated cells did not differ from that in control cells but decreased significantly in 200 μMBPAtreated cells. The mRNA level for brain-derived neurotrophic factor (Bdnf), which participates in GNRH1 secretory system development, decreased significantly in 200 μM BPA-treated cells, but that for neurotrophic tyrosine kinase, receptor, type 2 (Ntrk2), did not change. This indicates that Gnrh1 gene expression in mice fetuses is not affected by exposure to <20 μM BPA and that the adverse effects of BPA on the BDNF-NTRK2 neurotrophin system are induced by decrease in the mRNA level of the ligand, not of its receptor.
authors.affiliation: Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Kita-gun, Kagawa, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Warita
authors.forename: Tomoko
authors.initials: T
authors.lastname: Mitsuhashi
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Ohta
authors.forename: Shingo
authors.initials: S
authors.lastname: Suzuki
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Hoshi
authors.forename: Takanori
authors.initials: T
authors.lastname: Miki
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Takeuchi
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000494:pharmacology*; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics*; D005260:Female; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D007987:Gonadotropin-Releasing Hormone / Q000235:genetics*; D066298:In Vitro Techniques; D051379:Mice; D010636:Phenols / Q000494:pharmacology*; D011247:Pregnancy; D020813:Receptor, trkB / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051176:beta Catenin / Q000502:physiology; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D019208:Brain-Derived Neurotrophic Factor; D007987:Gonadotropin-Releasing Hormone; D010636:Phenols; D020813:Receptor, trkB; D051176:beta Catenin; C006780:bisphenol A; D000098826:Bisphenol A Compounds