title: Development of new polymorphic microsatellite loci for the barley stem gall midge, Mayetiola hordei (Diptera: Cecidomyiidae) from an enriched library.
abstract: Using an enriched library method, seven polymorphic microsatellite loci were isolated from the barley stem gall midge, Mayetiola hordei. Polymorphism at loci was surveyed on 57 individual midges collected on barley in Tunisia. Across loci, polymorphism ranged from two to six alleles per locus. The observed heterozygosity varied between 0.070 and 0.877. Based on the number of alleles detected and the associated levels of heterozygosity, we believe that these loci will prove useful for population genetic studies on M. hordei.
authors.affiliation: Genomics of Crop Insect Pests, Faculté des Sciences de Tunis, University of Tunis El-Manar, El Manar 2092, Tunisia. mahakm@planet.tn.
authors.forename: Maha
authors.initials: M
authors.lastname: Mezghani-Khemakhem
authors.forename: Dhia
authors.initials: D
authors.lastname: Bouktila
authors.forename: Nathalie
authors.initials: N
authors.lastname: Casse
authors.forename: Houcine
authors.initials: H
authors.lastname: Maaroufi
authors.forename: Mohamed
authors.initials: M
authors.lastname: Makni
authors.forename: Hanem
authors.initials: H
authors.lastname: Makni
mesh_terms: D000483:Alleles; D056426:Genetic Loci; D005838:Genotype; D001467:Hordeum / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D059372:Nucleotide Motifs; D018547:Plant Stems; D011110:Polymorphism, Genetic