title: Infectious bursal disease virus rescued efficiently with 3' authentic RNA sequence induces humoral immunity without bursal atrophy.
abstract: A reverse genetics infectious bursal disease virus (RG-IBDV) that contained authentic 3' RNA sequence was characterized both in vitro and in vivo. LP1-IBDV, a cell line-adapted IBDV strain variant E (VE) was used as the parent virus for constructing viral cDNA clones. Authentic 3' RNA sequence was generated by using cis-acting hepatitis delta virus ribozyme (HDR). The absence of HDR in the clones did not affect viral protein expression, but the obtained viral titers were reduced by 200-folds when compared to the clones with HDR sequence. RG-IBDV generated from clones with HDR sequence was similar to LP1-IBDV by plaque size, growth kinetics and electron microscopic morphology. RG-IBDV did not cause bursal atrophy in 3-week-old chickens at 3, 7 and 17 days post infection (DPI) and induced high ELISA and neutralizing antibody titers to IBDV at 7 and 17 DPI. The results indicated that RG-IBDV can be generated efficiently with an authentic 3' RNA terminus and the obtained RG-IBDV is non-pathogenic and immunogenic with the potential as a vaccine strain that can be further genetically modified to broaden its application.
authors.affiliation: Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Yung-Yi C
authors.initials: YY
authors.lastname: Mosley
authors.forename: Ching Ching
authors.initials: CC
authors.lastname: Wu
authors.forename: Tsang Long
authors.initials: TL
authors.lastname: Lin
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics* / Q000276:immunology; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood*; D018175:Birnaviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary / Q000821:virology; D002060:Bursa of Fabricius / Q000276:immunology / Q000473:pathology* / Q000821:virology; D002460:Cell Line; D002645:Chickens; D002522:Chlorocebus aethiops; D056724:Immunity, Humoral; D007243:Infectious bursal disease virus / Q000235:genetics* / Q000276:immunology / Q000502:physiology / Q000648:ultrastructure; D008854:Microscopy, Electron; D011201:Poultry Diseases / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D012367:RNA, Viral / Q000235:genetics* / Q000276:immunology; D014709:Vero Cells; D014765:Viral Vaccines
chemical_list: D020413:3' Untranslated Regions; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D012367:RNA, Viral; D014765:Viral Vaccines