title: Eri1 degrades the stem-loop of oligouridylated histone mRNAs to induce replication-dependent decay.
abstract: The exoRNase Eri1 inhibits RNA interference and trims the 5.8S rRNA 3' end. It also binds to the stem-loop of histone mRNAs, but the functional importance of this interaction remains elusive. Histone mRNAs are normally degraded at the end of S phase or after pharmacological inhibition of replication. Both processes are impaired in Eri1-deficient mouse cells, which instead accumulate oligouridylated histone mRNAs. Eri1 trims the mature histone mRNAs by two unpaired nucleotides at the 3' end but stalls close to the double-stranded stem. Upon oligouridylation of the histone mRNA, the Lsm1-7 heteroheptamer recognizes the oligo(U) tail and interacts with Eri1, whose catalytic activity is then able to degrade the stem-loop in a stepwise manner. These data demonstrate how degradation of histone mRNAs is initiated when 3' oligouridylation creates a cis element that enables Eri1 to process the double-stranded stem-loop structure.
authors.affiliation: Institute of Molecular Immunology, Helmholtz Zentrum München, München, Germany.
authors.forename: Kai P
authors.initials: KP
authors.lastname: Hoefig
authors.forename: Nicola
authors.initials: N
authors.lastname: Rath
authors.forename: Gitta A
authors.initials: GA
authors.lastname: Heinz
authors.forename: Christine
authors.initials: C
authors.lastname: Wolf
authors.forename: Jasmin
authors.initials: J
authors.lastname: Dameris
authors.forename: Aloys
authors.initials: A
authors.lastname: Schepers
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Kremmer
authors.forename: K Mark
authors.initials: KM
authors.lastname: Ansel
authors.forename: Vigo
authors.initials: V
authors.lastname: Heissmeyer
mesh_terms: D000818:Animals; D055162:Biocatalysis; D002453:Cell Cycle; D002478:Cells, Cultured; D005092:Exonucleases / Q000235:genetics / Q000378:metabolism*; D005095:Exoribonucleases; D005260:Female; D006657:Histones / Q000235:genetics* / Q000378:metabolism; D055029:Inverted Repeat Sequences; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000235:genetics / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology / Q000378:metabolism; D014500:Uracil Nucleotides / Q000378:metabolism
chemical_list: D006657:Histones; D009843:Oligoribonucleotides; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D014500:Uracil Nucleotides; C027916:oligo(U); C507007:Eri1 protein, mouse; D005092:Exonucleases; D005095:Exoribonucleases