title: Evaluation of an automated system for root canal irrigation: a scanning electron microscopy study.
abstract: This study evaluated a new automated system using alternative irrigants for root canal cleaning treatments. This method relies on a system inserting an enzymatic solution based on Trypsin flowing inside the pulp chambers and root canals, completely avoiding traditional endodontic instrumentation. Sixty freshly extracted human molar teeth were randomly divided into 4 groups to assess 3 regimens (R1-3) differing in 0.25% Trypsin/EDTA and 5% Sodium Hypochlorite (NaOCl) solutions administration. Scanning electron microscopy observations and scores taking into account changes in dentin tubules were used to assess treatment effects in pulp chambers and roots. Significant changes in root cleaning ability relative to administration timing were observed, with the best results found in R3, with scheduled alternated cycles of Trypsin/EDTA and NaOCl inside the tooth. The non-invasive root canal method demonstrates good teeth cleaning ability independent of root morphology. This equipment may provide lower discomfort levels for patients undergoing endodontic treatment.
authors.affiliation: Department of Clinical and Molecular Sciences, Università Politecnica delle Marche, Via Tronto 10/A, 60020 Ancona, Italy. m.mattioli@univpm.it
authors.forename: Monica
authors.initials: M
authors.lastname: Mattioli-Belmonte
authors.forename: Giovanna
authors.initials: G
authors.lastname: Orsini
authors.forename: Francesca
authors.initials: F
authors.lastname: Giuliodori
authors.forename: Adriano
authors.initials: A
authors.lastname: DI Cristoforo
authors.forename: Maurizio
authors.initials: M
authors.lastname: Procaccini
authors.forename: Paolo
authors.initials: P
authors.lastname: Mengucci
authors.forename: Angelo
authors.initials: A
authors.lastname: Putignano
mesh_terms: D001331:Automation; D003748:Dental Equipment; D004492:Edetic Acid; D006801:Humans; D008855:Microscopy, Electron, Scanning; D012388:Root Canal Irrigants / Q000008:administration & dosage*; D018915:Root Canal Preparation / Q000379:methods*; D012973:Sodium Hypochlorite; D014357:Trypsin
chemical_list: D012388:Root Canal Irrigants; D004492:Edetic Acid; D012973:Sodium Hypochlorite; D014357:Trypsin