title: Molecular alterations in meningiomas: association with clinical data.
abstract: The aim of our study was to evaluate the frequency of deletions on chromosomes 1, 9, 10, 14, 18 and 22 in 75 benign and 15 atypical meningiomas and correlate them with clinical findings. Paired normal and tumor DNA samples were analyzed for loss of heterozygosity (LOH), using 24 microsatellite markers and PCR techniques. Statistical analysis showed that deletions on chromosomes 14 and 18 were significantly associated with tumor grade of meningiomas (p = 0.048 and p = 0.03, respectively). In addition, we found a marginally increased frequency of LOH on chromosome 9 in atypical meningiomas (p = 0.06). Interestingly, LOH on chromosome 14 was significantly associated with tumor size (p = 0.049), as the risk of developing a tumor of more than 4 cm in diameter was 6-times the risk of developing tumor with diameter below 4 cm. The most frequent genetic abnormality in meningiomas is 22 LOH, which seems to be confirmed by the present study in which high frequency of such abnormality was observed (67%). We found associations between chromosome 22 status and histological subtype. LOH on chromosome 22 was more frequent in fibrous meningiomas than in the meningothelial variant (p = 0.001). Besides that, we found a relationship between 22 LOH status and tumor localization: the frequency of LOH in skull base-localized tumors was significantly lower compared to parasagittal meningiomas (p = 0.0004). Our results indicated that allelic loss on chromosomes 9, 10, 14, 18 and 22 may be associated with meningioma pathogenesis and progression.
authors.affiliation: Department of Neurosurgery, Medical University of Lodz, Poland. djask@o2.pl
authors.forename: Dariusz J
authors.initials: DJ
authors.lastname: Jaskolski
authors.forename: Sylwia M
authors.initials: SM
authors.lastname: Gresner
authors.forename: Magdalena
authors.initials: M
authors.lastname: Zakrzewska
authors.forename: Izabela
authors.initials: I
authors.lastname: Zawlik
authors.forename: Sylwester
authors.initials: S
authors.lastname: Piaskowski
authors.forename: Beata
authors.initials: B
authors.lastname: Sikorska
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Szybka
authors.forename: Wielislaw
authors.initials: W
authors.lastname: Papierz
authors.forename: Piotr
authors.initials: P
authors.lastname: Rieske
authors.forename: Pawel P
authors.initials: PP
authors.lastname: Liberski
mesh_terms: D002872:Chromosome Deletion; D005260:Female; D006801:Humans; D008297:Male; D008577:Meningeal Neoplasms / Q000235:genetics* / Q000473:pathology; D008579:Meningioma / Q000235:genetics* / Q000473:pathology; D008875:Middle Aged; D060787:Neoplasm Grading