title: Efficient cell reprogramming using bioengineered surfaces.
abstract: A novel method for cell reprogramming is been developed by immobilizing nucleic acid transfer vectors containing free amino groups, like lentiviral particles, onto pentafluorophenyl methacrylate (PFM)-modified surfaces obtained by PFM grafting affter Ar plasma treatment. This technique is able to reprogram murine somatic cells into pluripotent cells at high efficiencies. We call these modified surfaces cell reprogramming surfaces, or CRS.
authors.affiliation: Grup d'Enginyeria de Materials (GEMAT), Institut Químic de Sarrià-Universitat Ramon Llull, Via Augusta 390, 08017 Barcelona, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Horna
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Ramírez
authors.forename: Anna
authors.initials: A
authors.lastname: Cifuentes
authors.forename: Antonio
authors.initials: A
authors.lastname: Bernad
authors.forename: Salvador
authors.initials: S
authors.lastname: Borrós
authors.forename: Manuel A
authors.initials: MA
authors.lastname: González
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002460:Cell Line; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D008422:Materials Testing; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology*; D013499:Surface Properties; D014162:Transfection / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D004247:DNA