title: Modelling of biological Cr(VI) removal in draw-fill reactors using microorganisms in suspended and attached growth systems.
abstract: The kinetics of hexavalent chromium bio-reduction in draw-fill suspended and attached growth reactors was examined using sugar as substrate and indigenous microorganisms from the industrial sludge of the Hellenic Aerospace Industry. Initially, experiments in suspended growth batch reactors for Cr (VI) concentrations of 1.4-110 mg/l were carried out, to extensively study the behaviour of a mixed culture. The maximum Cr(VI) reduction rate of 2 mg/l h was achieved for initial concentration 12.85 mg/l with biomass production rate 4.1 mg biomass/l h. Analysis of the microbial structure in the batch reactor culture indicated that the dominant bacterial communities were constituted by bacterial members of Raoultella sp., Citrobacter sp., Klebsiella sp., Salmonella sp., Achromobacter sp. and Kerstersia sp. while the dominant fungal strain was that of Pichia jadinii. Experiments using the same mixed culture were also carried out in packed-bed reactors with plastic support media. High removal rates were achieved (2.0 mg/l h) even in high initial concentrations (109 mg/l). A combination of the model of Tsao and Hanson for growth enhancement and that of Aiba and Shoda for growth inhibition was used in order to describe and predict the process of Cr(VI) bio-reduction in suspended growth and packed-bed reactors. Kinetic constants of the equation obtained from both batch (or draw-fill) culture experiments. In the draw-fill experiments at the packed-bed reactor, hexavalent chromium inhibitory effects were minimized increasing the inhibitory constant value K(i)' at 148.5 mg/l, compared to suspended growth experiments which was K(i) = 8.219 mg/l. The model adequately predicts hexavalent chromium reduction in both batch reactors for all initial concentrations tested.
authors.affiliation: Department of Environmental and Natural Resources Management, University of Western Greece, 2 Seferi Str., 30100 Agrinio, Greece. atekerle@uwg.gr
authors.forename: Athanasia G
authors.initials: AG
authors.lastname: Tekerlekopoulou
authors.forename: Maria
authors.initials: M
authors.lastname: Tsiflikiotou
authors.forename: Lydia
authors.initials: L
authors.lastname: Akritidou
authors.forename: Anastasios
authors.initials: A
authors.lastname: Viennas
authors.forename: George
authors.initials: G
authors.lastname: Tsiamis
authors.forename: Stavros
authors.initials: S
authors.lastname: Pavlou
authors.forename: Kostas
authors.initials: K
authors.lastname: Bourtzis
authors.forename: Dimitris V
authors.initials: DV
authors.lastname: Vayenas
mesh_terms: D042402:Achromobacter / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D042401:Alcaligenaceae / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D018441:Biofilms / Q000254:growth & development; D019149:Bioreactors / Q000191:economics / Q000382:microbiology*; D018914:Cells, Immobilized / Q000378:metabolism; D002857:Chromium / Q000032:analysis / Q000378:metabolism*; D004755:Enterobacteriaceae / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D005240:Feasibility Studies; D006115:Greece; D007220:Industrial Waste / Q000032:analysis / Q000191:economics; D007700:Kinetics; D008954:Models, Biological; D009994:Osmolar Concentration; D010843:Pichia / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D010865:Pilot Projects; D011192:Potassium Dichromate / Q000737:chemistry; D013026:Space Flight / Q000191:economics; D013395:Sucrose / Q000378:metabolism; D062065:Wastewater / Q000737:chemistry; D018508:Water Purification / Q000191:economics / Q000379:methods
chemical_list: D002857:Chromium; D007220:Industrial Waste; D011192:Potassium Dichromate; D013395:Sucrose; D062065:Wastewater