title: Quantitative surface-enhanced resonant Raman scattering multiplexing of biocompatible gold nanostars for in vitro and ex vivo detection.
abstract: Surface-enhanced Raman scattering (SERS)-active plasmonic nanomaterials have become a promising agent for molecular imaging and multiplex detection. To produce strong SERS intensity while retaining the nonaggregated state and biocompatibility needed for bioapplications, we integrated near-infrared (NIR) responsive plasmonic gold nanostars with resonant dyes for resonant SERS (SERRS). The SERRS on nanostars was several orders of magnitude greater than signals from SERRS on nanospheres and nonresonant SERS on nanostars. For the first time, we demonstrated quantitative multiplex detection using four unique nanostar SERRS probes in both in vitro solutions and ex vivo tissue samples under NIR excitation. With further optimization, in vivo tracking of multiple SERRS probes is possible.
authors.affiliation: Fitzpatrick Institute of Photonics, Departments of Biomedical Engineering and Chemistry, Duke University, Durham, North Carolina 27708, United States.
authors.forename: Hsiangkuo
authors.initials: H
authors.lastname: Yuan
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Fales
authors.forename: You Leo
authors.initials: YL
authors.lastname: Li
authors.forename: Jesse
authors.initials: J
authors.lastname: Liu
authors.forename: Tuan
authors.initials: T
authors.lastname: Vo-Dinh
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000032:analysis*; D002417:Cattle; D002645:Chickens; D005456:Fluorescent Dyes / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012867:Skin / Q000737:chemistry; D013059:Spectrum Analysis, Raman
chemical_list: D001672:Biocompatible Materials; D005456:Fluorescent Dyes; D012710:Serum Albumin, Bovine; D006046:Gold