title: Selective recognition of G-G mismatch using the double functional probe with electrochemical active ferrocenyl.
abstract: A new electrochemical assaying approach with label-free to identify GG mismatch in DNA duplexes was developed by applying a new double functional probe (FecNC), ferrocenyl modified naphthyridine derivative, which contain recognition domain (naphthyridine derivative) and electroactive center (ferrocenyl group). The double functional probe exhibited not only the excellent electrochemical response devoted from ferrocenyl but also high selective electrochemical signal "off" for G-G mismatch duplex. The interaction was also verified by melting temperature and circular dichroismic spectra (CD). The electrochemical investigation showed that the redox of FecNC was a diffusion-controlled process and the diffusion coefficient of bound-FecNC was much smaller than free FecNC. The correlation between the current of FecNC and concentration of GG mismatch duplex indicated that the bind saturation point was about at a 1:1 mole ratio. The novel double functional electrochemical probe might provide a versatile and low-cost way to detect single nucleotide polymorphisms, which could be found extensive applications in the diagnosis of the genetic diseases.
authors.affiliation: Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Youyi Road 368, Wuchang, Wuhan, Hubei 430062, PR China. hehanping@gmail.com
authors.forename: Hanping
authors.initials: H
authors.lastname: He
authors.forename: Jingping
authors.initials: J
authors.lastname: Xia
authors.forename: Gang
authors.initials: G
authors.lastname: Chang
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Peng
authors.forename: Zhaowen
authors.initials: Z
authors.lastname: Lou
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakatani
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
authors.forename: Shengfu
authors.initials: S
authors.lastname: Wang
mesh_terms: D020137:Base Pair Mismatch / Q000235:genetics*; D015374:Biosensing Techniques; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D055664:Electrochemical Techniques; D005296:Ferrous Compounds / Q000737:chemistry*; D006801:Humans; D009287:Naphthyridines / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics
chemical_list: D005296:Ferrous Compounds; D009287:Naphthyridines; D004247:DNA