title: GFT projection NMR for efficient (1)H/          (13)C sugar spin system identification in nucleic acids.
abstract: A newly implemented G-matrix Fourier transform (GFT) (4,3)D HC(C)CH experiment is presented in conjunction with (4,3)D HCCH to efficiently identify (1)H/(13)C sugar spin systems in (13)C labeled nucleic acids. This experiment enables rapid collection of highly resolved relay 4D HC(C)CH spectral information, that is, shift correlations of (13)C-(1)H groups separated by two carbon bonds. For RNA, (4,3)D HC(C)CH takes advantage of the comparably favorable 1'- and 3'-CH signal dispersion for complete spin system identification including 5'-CH. The (4,3)D HC(C)CH/HCCH based strategy is exemplified for the 30-nucleotide 3'-untranslated region of the pre-mRNA of human U1A protein.
authors.affiliation: NMR Research Centre, Indian Institute of Science, Bangalore, India.
authors.forename: Hanudatta S
authors.initials: HS
authors.lastname: Atreya
authors.forename: Bharathwaj
authors.initials: B
authors.lastname: Sathyamoorthy
authors.forename: Garima
authors.initials: G
authors.lastname: Jaipuria
authors.forename: Victor
authors.initials: V
authors.lastname: Beaumont
authors.forename: Gabriele
authors.initials: G
authors.lastname: Varani
authors.forename: Thomas
authors.initials: T
authors.lastname: Szyperski
mesh_terms: D002247:Carbon Isotopes; D003903:Deuterium; D005583:Fourier Analysis; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009696:Nucleic Acids / Q000737:chemistry*; D012322:RNA Precursors / Q000737:chemistry*; D017412:Ribonucleoprotein, U1 Small Nuclear / Q000737:chemistry
chemical_list: D002247:Carbon Isotopes; D009696:Nucleic Acids; D012322:RNA Precursors; D017412:Ribonucleoprotein, U1 Small Nuclear; C075339:U1A protein; D003903:Deuterium