title: Magnetic resonance imaging and fluorescence labeling of clinical-grade mesenchymal stem cells without impacting their phenotype: study in a rat model of stroke.
abstract: Human mesenchymal stem cells (hMSCs) have strong potential for cell therapy after stroke. Tracking stem cells in vivo following a graft can provide insight into many issues regarding optimal route and/or dosing. hMSCs were labeled for magnetic resonance imaging (MRI) and histology with micrometer-sized superparamagnetic iron oxides (M-SPIOs) that contained a fluorophore. We assessed whether M-SPIO labeling obtained without the use of a transfection agent induced any cell damage in clinical-grade hMSCs and whether it may be useful for in vivo MRI studies after stroke. M-SPIOs provided efficient intracellular hMSC labeling and did not modify cell viability, phenotype, or in vitro differentiation capacity. Following grafting in a rat model of stroke, labeled hMSCs could be detected using both in vivo MRI and fluorescent microscopy until 4 weeks following transplantation. However, whereas good label stability and unaffected hMSC viability were observed in vitro, grafted hMSCs may die and release iron particles in vivo.
authors.affiliation: Institut National de Santé et de Recherche Médicale, Grenoble, France. odetante@chu-grenoble.fr
authors.forename: Olivier
authors.initials: O
authors.lastname: Detante
authors.forename: Samuel
authors.initials: S
authors.lastname: Valable
authors.forename: Florence
authors.initials: F
authors.lastname: de Fraipont
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Grillon
authors.forename: Emmanuel Luc
authors.initials: EL
authors.lastname: Barbier
authors.forename: Anaïck
authors.initials: A
authors.lastname: Moisan
authors.forename: Josiane
authors.initials: J
authors.lastname: Arnaud
authors.forename: Christine
authors.initials: C
authors.lastname: Moriscot
authors.forename: Christoph
authors.initials: C
authors.lastname: Segebarth
authors.forename: Marc
authors.initials: M
authors.lastname: Hommel
authors.forename: Chantal
authors.initials: C
authors.lastname: Remy
authors.forename: Marie-Jeanne
authors.initials: MJ
authors.lastname: Richard
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005290:Ferric Compounds / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000473:pathology*; D008856:Microscopy, Fluorescence / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D020521:Stroke / Q000473:pathology* / Q000628:therapy*; D014183:Transplantation, Heterologous
chemical_list: D005290:Ferric Compounds; D005456:Fluorescent Dyes; C000499:ferric oxide