title: Nanospiderwebs: artificial 3D extracellular matrix from nanofibers by novel clinical grade electrospinning for stem cell delivery.
abstract: Novel clinical grade electrospinning methods could provide three-dimensional (3D) nanostructured biomaterials comprising of synthetic or natural biopolymer nanofibers. Such advanced materials could potentially mimic the natural extracellular matrix (ECM) accurately and may provide superior niche-like spaces on the subcellular scale for optimal stem-cell attachment and individual cell homing in regenerative therapies. The goal of this study was to design several novel "nanofibrous extracellular matrices" (NF-ECMs) with a natural mesh-like 3D architecture through a unique needle-free multi-jet electrospinning method in highly controlled manner to comply with good manufacturing practices (GMP) for the production of advanced healthcare materials for regenerative medicine, and to test cellular behavior of human mesenchymal stem cells (HMSCs) on these. Biopolymers manufactured as 3D NF-ECM meshes under clinical grade GMP-like conditions show higher intrinsic cytobiocompatibility with superior cell integration and proliferation if compared to their 2D counterparts or a clinically-approved collagen membrane.
authors.affiliation: Clem Jones Research Centre For Stem Cells & Tissue Regenerative Therapies, Bond University, Gold Coast, QLD, 4229, Australia.
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: Alamein
authors.forename: Qin
authors.initials: Q
authors.lastname: Liu
authors.forename: Sebastien
authors.initials: S
authors.lastname: Stephens
authors.forename: Stuart
authors.initials: S
authors.lastname: Skabo
authors.forename: Frauke
authors.initials: F
authors.lastname: Warnke
authors.forename: Robert
authors.initials: R
authors.lastname: Bourke
authors.forename: Peter
authors.initials: P
authors.lastname: Heiner
authors.forename: Patrick H
authors.initials: PH
authors.lastname: Warnke
mesh_terms: D040761:Biomimetic Materials / Q000138:chemical synthesis; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D004563:Electrochemistry / Q000379:methods*; D005109:Extracellular Matrix / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D012399:Rotation; D054457:Tissue Scaffolds