title: Isolation and characterization of a human intestinal bacterium Eggerthella sp. CAT-1 capable of cleaving the C-ring of (+)-catechin and (-)-epicatechin, followed by p-dehydroxylation of the B-ring.
abstract: We isolated a human intestinal bacterium, capable of cleaving the C-ring and dehydroxylating the B-ring of both (+)-catechin (2R,3S) and (-)-epicatechin (2R,3R). Although the strain was classified as Eggerthella (Eg.) lenta [named Eg. sp. CAT-1 (JF798636)] by 16S ribosomal RNA (rRNA) gene similarity, it was quite different in substrate specificity from a previously isolated strain, Eg. sp. SDG-2, which takes part in cleavage of the C-ring and dehydroxylation of the 3,4-dihydroxyphenyl moiety (B-ring) of (3R)-flavan-3-ol derivatives. On the other hand, both Eg. sp. CAT-1 and Eg. sp. SDG-2 showed the same substrate specificity against dehydroxylation of enantiomeric lignans, (+)- and (-)-dihydroxyenterodiol, and (+)- and (-)-dihydroxyenterolactone.
authors.affiliation: Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930–0194, Japan.
authors.forename: Jong-Sik
authors.initials: JS
authors.lastname: Jin
authors.forename: Masao
authors.initials: M
authors.lastname: Hattori
mesh_terms: D039903:Actinobacteria / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D002392:Catechin / Q000378:metabolism*; D005419:Flavonoids / Q000378:metabolism; D005798:Genes, Bacterial; D006801:Humans; D006900:Hydroxylation; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000382:microbiology*; D017705:Lignans / Q000378:metabolism; D012336:RNA, Ribosomal, 16S; D013045:Species Specificity
chemical_list: D005419:Flavonoids; D017705:Lignans; D012336:RNA, Ribosomal, 16S; C404987:flavan-3-ol; D002392:Catechin