title: Development of an analytical approach for identification and quantification of 5-α-androst-16-en-3-one in human milk.
abstract: A method was developed for the quantification of 5-α-androst-16-en-3-one in human breast milk based on application of a stable isotope dilution assay using 5α-androst-16-en-3-one-6, 6-d(2). The procedure includes extraction of the human milk by hexane with subsequent clean-up of the obtained extract by gel permeation and silica gel column chromatography. The extracted samples were analyzed by gas chromatography-mass spectrometry. Using this method 5-α-androst-16-en-3-one could be identified and for the first time quantified in a concentration range of 26-155 ng/kg in human milk.
authors.affiliation: Department of Chemistry and Pharmacy, Food Chemistry, Emil Fischer Center, University of Erlangen-Nuremberg, 91052 Erlangen, Germany.
authors.forename: Constanze
authors.initials: C
authors.lastname: Hartmann
authors.forename: Fabienne
authors.initials: F
authors.lastname: Mayenzet
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Larcinese
authors.forename: Olivier P
authors.initials: OP
authors.lastname: Haefliger
authors.forename: Andrea
authors.initials: A
authors.lastname: Buettner
authors.forename: Christian
authors.initials: C
authors.lastname: Starkenmann
mesh_terms: D000736:Androstenes / Q000032:analysis* / Q000737:chemistry; D002850:Chromatography, Gel; D003903:Deuterium; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007553:Isotope Labeling; D008895:Milk, Human / Q000737:chemistry*; D013194:Staining and Labeling
chemical_list: D000736:Androstenes; C000214:androst-16-en-3-one; D003903:Deuterium