title: Longitudinal analysis of protein glycosylation and β-casein phosphorylation in term and preterm human milk during the first 2 months of lactation.
abstract: Human milk proteins provide term and preterm infants with both nutrition and protection. The objective of the present study was to examine longitudinal changes in the protein composition of term and preterm milk during the first 2 months of lactation, focusing on protein phosphorylation and glycosylation. Using gel electrophoresis, the relative concentration and glycosylation status of lactoferrin, secretory Ig A, β-casein, α-lactalbumin, serum albumin, bile salt-stimulated lipase, xanthine oxidoreductase, tenascin and macrophage mannose receptor 1 were measured in milk collected on days 7, 10, 14, 18, 21, 28 and 60 postpartum from preterm mothers (28-32 weeks gestation, n 17). The phosphorylation status of β-casein was also investigated. To determine if these variables differ in term and preterm milk, samples from term mothers (38-41 weeks gestation, n 8) collected on days 7, 14 and 30 of lactation were also analysed. The concentration of the abundant milk proteins decreased during lactation in term and preterm milk (P <0·05). No difference in protein glycosylation was observed, except for the glycoproteins serum albumin and tenascin. The phosphorylation of β-casein varied significantly between term and preterm milk. Further investigation is required to determine whether these modifications affect protein function and are clinically important to preterm infants.
authors.affiliation: School of Chemistry and Biochemistry, The University of Western Australia, M310, 35 Stirling Highway, Crawley, WA 6009, Australia. 10224872@student.uwa.edu.au
authors.forename: Claire E
authors.initials: CE
authors.lastname: Molinari
authors.forename: Ylenia S
authors.initials: YS
authors.lastname: Casadio
authors.forename: Ben T
authors.initials: BT
authors.lastname: Hartmann
authors.forename: Peter G
authors.initials: PG
authors.lastname: Arthur
authors.forename: Peter E
authors.initials: PE
authors.lastname: Hartmann
mesh_terms: D000293:Adolescent; D000328:Adult; D002364:Caseins / Q000378:metabolism*; D004044:Dietary Proteins / Q000378:metabolism; D005260:Female; D005865:Gestational Age; D006031:Glycosylation; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D007774:Lactation / Q000378:metabolism*; D008137:Longitudinal Studies; D008894:Milk Proteins / Q000378:metabolism*; D008895:Milk, Human / Q000378:metabolism*; D010766:Phosphorylation; D012709:Serum Albumin / Q000378:metabolism; D019063:Tenascin / Q000378:metabolism; D055815:Young Adult
chemical_list: D002364:Caseins; D004044:Dietary Proteins; D008894:Milk Proteins; D012709:Serum Albumin; D019063:Tenascin