title: Relation of oxidant-antioxidant imbalance with disease progression in patients with asthma.
abstract: BACKGROUND
  Asthma is a chronic airway disorder which is associated to the inflammatory cells. Inflammatory and immune cells generate more reactive oxygen species in patients suffering from asthma which leads to tissue injury.
  
  
  OBJECTIVE
  To investigate the role of oxidant-antioxidant imbalance in disease progression of asthmatic patients.
  
  
  METHODS
  In this study, 130 asthmatic patients and 70 healthy controls were documented.
  
  
  METHODS
  For this malondialdehyde level, total protein carbonyls, sulfhydryls, activity of superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), total blood glutathione, and total antioxidant capacity (FRAP) were measured.
  
  
  METHODS
  Analysis of the data was done using unpaired student t test and one-way ANOVA analysis. P < 0.05 was considered significant.
  
  
  RESULTS
  The present work showed that the systemic levels of MDA (4.19 ± 0.10 nmol/ml, P < 0.001) and protein carbonyls (1.13 ± 0.02 nmol/mg, P < 0.001) were found to be remarkably higher in asthmatic patients while protein sulfhydryls (0.55 ± 0.01 mmol/l, P < 0.05) decreased as compared to controls (2.84 ± 0.12 nmol/ml, 0.79 ± 0.02 nmol/mg and 0.60 ± 0.02 mmol/l, respectively). We also observed decrease in activities of SOD (2047 ± 50.34 U/g Hb, P < 0.05), catalase (4374 ± 67.98 U/g Hb, P < 0.01), and GPx (40.97 ± 1.05 U/g Hb, P < 0.01) in erythrocytes compared to control (2217 ± 60.11 U/g Hb, 4746 ± 89.94 U/g Hb, and 48.37 ± 2.47 U/g Hb, respectively). FRAP level (750.90 ± 21.22 μmol/l, P < 0.05) in plasma was decreased, whereas total blood glutathione increased (0.94 ± 0.02 mmol/l, P < 0.05) as seen in control (840.40 ± 28.39 μmol/l and 0.84 ± 0.04 mmol/l).
  
  
  CONCLUSIONS
  This work supports and describes the hypothesis that an imbalance between oxidant-antioxidant is associated to the oxidative stress which plays a significant role in severity of the disease.
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, India ; Department of T.B. and Respiratory Diseases, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India.
authors.forename: Asrar
authors.initials: A
authors.lastname: Ahmad
authors.forename: Mohammad
authors.initials: M
authors.lastname: Shameem
authors.forename: Qayyum
authors.initials: Q
authors.lastname: Husain