title: Inhibitory effects of the attenuated Salmonella typhimurium containing the IL-2 gene on hepatic tumors in mice.
abstract: To observe the inhibitory effects of an attenuated S. typhimurium strain carrying IL-2 gene (TPI) on hepatoma cell line (HepG2) and transplanted tumors in mice. TPI, TPG (an attenuated S. typhimurium strain carrying green fluorescent protein gene), and TP (an attenuated S. typhimurium strain) strains were transfected into HepG2 cells. At 48 h after transfecting, the transfection rate was 82.58 ± 1.74%. The expression level of IL-2 was (99.5 ± 12.2) ng/1 × 10(6) cells. Compared with TPG, TP, and normal mouse groups, the proportion of CD4(+) T and CD8(+) T cells in the blood from the TPI group was higher, the levels of IgM and IgG(1) were significantly increased, and the proliferation activity of splenic lymphocyte was significantly stronger. The transplanted tumor weight in the TPI group was significantly smaller than that in the other two groups. The infiltration of lymphocytes increased in the tumor from TPI group mice. TPI was effectively transfected into cancer cells, which expressed the protein of interest. Oral administration of TPI prolonged survival of mice transplanted with hepatoma cell tumours.
authors.affiliation: Department of Clinical Laboratory Medicine, Lanzhou General Hospital of Lanzhou Military Region, People's Liberation Army, Key Laboratory of Stem Cell and Gene Drug in Gansu Province, Lanzhou 730050, China. haxq@yahoo.com
authors.forename: Xiao-qin
authors.initials: XQ
authors.lastname: Ha
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yin
authors.forename: Hong-bin
authors.initials: HB
authors.lastname: Zhao
authors.forename: Ling
authors.initials: L
authors.lastname: Hui
authors.forename: Mei-liang
authors.initials: ML
authors.lastname: Wang
authors.forename: Jun-hua
authors.initials: JH
authors.lastname: Peng
authors.forename: Ju-zi
authors.initials: JZ
authors.lastname: Dong
authors.forename: Zhi-yun
authors.initials: ZY
authors.lastname: Deng
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Zhang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D049109:Cell Proliferation; D003587:Cytomegalovirus / Q000235:genetics; D015870:Gene Expression; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D007136:Immunoglobulins / Q000097:blood / Q000276:immunology; D007376:Interleukin-2 / Q000235:genetics* / Q000627:therapeutic use*; D008113:Liver Neoplasms / Q000097:blood / Q000276:immunology / Q000628:therapy*; D016131:Lymphocyte Subsets / Q000276:immunology; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology / Q000473:pathology; D051379:Mice; D011401:Promoter Regions, Genetic / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics; D012486:Salmonella typhimurium / Q000502:physiology*; D013154:Spleen / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology; D014018:Tissue Distribution; D014162:Transfection; D023041:Xenograft Model Antitumor Assays
chemical_list: D007136:Immunoglobulins; D007376:Interleukin-2; D049452:Green Fluorescent Proteins