title: Co-culture with endothelial progenitor cells promotes survival, migration, and differentiation of osteoclast precursors.
abstract: In this study, we report the effect of endothelial progenitor cells (EPCs) on the biological behavior of osteoclast precursors in vitro by establishing an indirect co-culture system of mice EPCs and RAW 264.7 monocyte cells. Results show that the survival, migration, and differentiation of osteoclast precursors were greatly enhanced when co-cultured with EPCs. These phenotypic changes coincide with the upregulation of multiple genes affected cell behavior, including phospho-VEGFR-2, CXCR4, phospho-Smad2/3, phospho-Akt, phospho-ERK1, and phospho-p38 MAPK. The results collectively suggest that EPCs could modulate the survival, migration, and differentiation potential of osteoclast precursors, thus providing new insights in understanding of correlation between angiogenesis and bone homeostasis.
authors.affiliation: Department of Orthopaedics, Southwest Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Pang
authors.forename: Xue-Hui
authors.initials: XH
authors.lastname: Wu
authors.forename: Sheng-Long
authors.initials: SL
authors.lastname: Fu
authors.forename: Fei
authors.initials: F
authors.lastname: Luo
authors.forename: Ze-Hua
authors.initials: ZH
authors.lastname: Zhang
authors.forename: Tian-Yong
authors.initials: TY
authors.lastname: Hou
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Li
authors.forename: Zheng-Qi
authors.initials: ZQ
authors.lastname: Chang
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Xu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D002469:Cell Separation; D002470:Cell Survival / Q000235:genetics / Q000502:physiology; D042783:Endothelial Cells / Q000166:cytology*; D005786:Gene Expression Regulation; D051379:Mice; D008810:Mice, Inbred C57BL; D010010:Osteoclasts / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*