title: Quantitative analysis of total amino acid in barley leaves under herbicide stress using spectroscopic technology and chemometrics.
abstract: Visible and near infrared (Vis/NIR) spectroscopy were employed for the fast and nondestructive estimation of the total amino acid (TAA) content in barley (Hordeum vulgare L.) leaves. The calibration set was composed of 50 samples; and the remaining 25 samples were used for the validation set. Seven different spectral preprocessing methods and six different calibration methods (linear and nonlinear) were applied for a comprehensive prediction performance comparison. Successive projections algorithm (SPA) and regression coefficients (RC) were applied to select effective wavelengths (EWs). The results indicated that the latent variables-least-squares-support vector machine (LV-LS-SVM) model achieved the optimal performance. The prediction results by LV-LS-SVM with raw spectra were achieved with a correlation coefficients (r) = 0.937 and root mean squares error of prediction (RMSEP) = 0.530. The overall results showed that the NIR spectroscopy could be used for determination of TAA content in barley leaves with an excellent prediction precision; and the results were also helpful for on-field monitoring of barley growing status under herbicide stress during different growth stages.
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. ydbao@zju.edu.cn
authors.forename: Yidan
authors.initials: Y
authors.lastname: Bao
authors.forename: Wenwen
authors.initials: W
authors.lastname: Kong
authors.forename: Yong
authors.initials: Y
authors.lastname: He
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.forename: Tian
authors.initials: T
authors.lastname: Tian
authors.forename: Weijun
authors.initials: W
authors.lastname: Zhou
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000187:drug effects; D002623:Chemistry Techniques, Analytical / Q000379:methods; D004353:Drug Evaluation, Preclinical; D006540:Herbicides / Q000494:pharmacology*; D001467:Hordeum / Q000737:chemistry* / Q000187:drug effects*; D018515:Plant Leaves / Q000737:chemistry / Q000187:drug effects; D010940:Plant Proteins / Q000032:analysis / Q000187:drug effects; D019265:Spectroscopy, Near-Infrared / Q000379:methods; D013312:Stress, Physiological / Q000187:drug effects
chemical_list: D000596:Amino Acids; D006540:Herbicides; D010940:Plant Proteins