title: Inhibition of enzyme activity of Rhipicephalus (Boophilus) microplus triosephosphate isomerase and BME26 cell growth by monoclonal antibodies.
abstract: In the present work, we produced two monoclonal antibodies (BrBm37  and BrBm38) and tested their action against the triosephosphate isomerase of  Rhipicephalus (Boophilus) microplus (RmTIM). These antibodies recognize epitopes on both the native and recombinant forms of the protein. rRmTIM inhibition&nbsp; by BrBm37 was up to 85% whereas that of BrBrm38 was 98%, depending on the antibody-enzyme ratio. RmTIM activity was lower in ovarian, gut, and fat body tissue extracts treated with BrBm37 or BrBm38 mAbs. The proliferation of the embryonic tick cell line (BME26) was inhibited by BrBm37 and BrBm38 mAbs. In summary, the results reveal that it is possible to interfere with the RmTIM function using antibodies, even in intact cells.
authors.affiliation: Laboratory of Biochemistry Hatisaburo Masuda, Institute of Medical Biochemistry,  Federal University of Rio de Janeiro, NUPEM - UFRJ/Macaé, Av. São José do Barreto 764,  São José do Barreto, Macaé, RJ, CEP 27971-550, Brazil. jorgemoraes@bioqmed.ufrj.br.
authors.forename: Luiz
authors.initials: L
authors.lastname: Saramago
authors.forename: Mariana
authors.initials: M
authors.lastname: Franceschi
authors.forename: Carlos
authors.initials: C
authors.lastname: Logullo
authors.forename: Aoi
authors.initials: A
authors.lastname: Masuda
authors.forename: Itabajara da Silva
authors.initials: Ida S
authors.lastname: Vaz
authors.forename: Sandra Estrazulas
authors.initials: SE
authors.lastname: Farias
authors.forename: Jorge
authors.initials: J
authors.lastname: Moraes
mesh_terms: D000273:Adipose Tissue / Q000201:enzymology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002460:Cell Line; D049109:Cell Proliferation; D005260:Female; D007422:Intestines / Q000201:enzymology; D010053:Ovary / Q000201:enzymology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D048494:Rhipicephalus / Q000201:enzymology*; D014305:Triose-Phosphate Isomerase / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins; D014305:Triose-Phosphate Isomerase