title: New TFA-free cleavage and final deprotection in fmoc solid-phase peptide synthesis: dilute HCl in fluoro alcohol.
abstract: A novel method for cleaving from resin and removing acid-labile protecting groups for the Fmoc solid-phase peptide synthesis is described. 0.1 N HCl in hexafluoroisopropanol or trifluoroethanol cleanly and rapidly removes the tert-butyl ester and ether, Boc, trityl, and Pbf groups and cleaves the common resin linkers: Wang, HMPA, Rink amide, and PAL. Addition of just 5-10% of a hydrogen-bonding solvent considerably retards or even fully inhibits the reaction. However, a non-hydrogen-bonding solvent is tolerated.
authors.affiliation: Department of Chemistry, University of Reading, P.O. Box 224, Whiteknights, Reading RG6 6AD, UK.
authors.forename: Pasquale
authors.initials: P
authors.lastname: Palladino
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Stetsenko
mesh_terms: D004952:Esters; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D012117:Resins, Synthetic / Q000737:chemistry*; D060327:Solid-Phase Synthesis Techniques; D014269:Trifluoroacetic Acid / Q000737:chemistry*
chemical_list: D004952:Esters; D010455:Peptides; D012117:Resins, Synthetic; D014269:Trifluoroacetic Acid