title: Spectroscopic determination of lysozyme conformational changes in the presence of trehalose and guanidine.
abstract: The bioprotective action of the disaccharide trehalose has been studied against the well-known denaturating agent, guanidine hydrochloride. The results indicated a direct influence of trehalose on both enzymatic activity and conformational changes of lysozyme, as shown by the decrease of the inactivation rate constant of about 1.48-fold and the loss of α-helix structure of lysozyme. In addition, ESI-MS hydrogen-deuterium (H/D) exchange experiments allowed us to correlate the structural and dynamic features of the protein in the presence of the two additives, highlighting as trehalose remarkably influenced this exchange by decreasing local protein environment changes and solvent accessibility to the amide peptide backbone, as further evidenced by circular dichroism and (1)H NMR measurements.
authors.affiliation: Department of Organic and Biological Chemistry, University of Messina, Viale F. Stagno d'Alcontres 31, 98166, Messina, Italy. dbarreca@unime.it
authors.forename: Davide
authors.initials: D
authors.lastname: Barreca
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Laganà
authors.forename: Silvana
authors.initials: S
authors.lastname: Ficarra
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Gattuso
authors.forename: Salvatore
authors.initials: S
authors.lastname: Magazù
authors.forename: Roberto
authors.initials: R
authors.lastname: La Torre
authors.forename: Ester
authors.initials: E
authors.lastname: Tellone
authors.forename: Ersilia
authors.initials: E
authors.lastname: Bellocco
mesh_terms: D000818:Animals; D002645:Chickens; D002942:Circular Dichroism; D041961:Deuterium Exchange Measurement; D019791:Guanidine / Q000737:chemistry*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D009113:Muramidase / Q000737:chemistry*; D010063:Ovum / Q000201:enzymology; D058849:Protein Refolding; D017434:Protein Structure, Tertiary; D012997:Solvents / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013816:Thermodynamics; D014199:Trehalose / Q000737:chemistry*
chemical_list: D012997:Solvents; D014199:Trehalose; D009113:Muramidase; D019791:Guanidine