title: Time-regulated drug delivery system based on coaxially incorporated platelet α-granules for biomedical use.
abstract: OBJECTIVE
  Platelet derivatives serve as an efficient source of natural growth factors. In the current study, α-granules were incorporated into coaxial nanofibers.
  
  
  METHODS
  A nanofiber scaffold containing α-granules was prepared by coaxial electrospinning. The biological potential of the nanofiber scaffold was evaluated in chondrocyte and mesenchymal stem cell cultivation studies. Additionally, the concentration of TGF-β1 was determined.
  
  
  RESULTS
  Microscopy studies showed that intact α-granules were incorporated into the coaxial nanofibers. The cultivation tests showed that the novel scaffold stimulated viability and extracellular matrix production of chondrocytes and mesenchymal stem cells. In addition, the concentration of growth factors necessary for the induction of cell proliferation significantly decreased.
  
  
  CONCLUSIONS
  The system preserved α-granule bioactivity and stimulated cell viability and chondrogenic differentiation of mesenchymal stem cells. Core/shell nanofibers incorporating α-granules are a promising system for tissue engineering, particularly cartilage engineering.
authors.affiliation: Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, V Úvalu 84, Prague 5, Czech Republic.
authors.forename: Matej
authors.initials: M
authors.lastname: Buzgo
authors.forename: Radka
authors.initials: R
authors.lastname: Jakubova
authors.forename: Andrea
authors.initials: A
authors.lastname: Mickova
authors.forename: Michala
authors.initials: M
authors.lastname: Rampichova
authors.forename: Eva
authors.initials: E
authors.lastname: Prosecka
authors.forename: Petra
authors.initials: P
authors.lastname: Kochova
authors.forename: David
authors.initials: D
authors.lastname: Lukas
authors.forename: Evžen
authors.initials: E
authors.lastname: Amler
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects; D003594:Cytoplasmic Granules / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D018613:Microscopy, Confocal; D057139:Nanofibers / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry; D053773:Transforming Growth Factor beta1
chemical_list: D053773:Transforming Growth Factor beta1