title: Simultaneous determination of 4-substituted cathinones (4-MMC, 4-MEC and 4-FMC) in human urine by HPLC-DAD.
abstract: This study developed a selective and rapid high-performance liquid chromatography-diode array detection method for the confirmation of different cathinone derivates in human urine. Samples were prepared by solid-phase extraction (SPE) using procaine hydrochloride as the internal standard. The chromatographic separation was performed on a Kinetex PFP column using isocratic elution. The mobile phase was composed of a mixture of acetonitrile (33%, v/v) and 0.005M ammonium trifluoroacetate buffer (67%, v/v; pH 4.93 ± 0.03) with a flow rate of 0.350 mL/min. The diode array detection was performed at 262 nm. The method was linear over the concentration range of 25-2,400 ng/mL. Intra-day and inter-day precision values for cathinones were less than 1.26% (relative standard deviation). The limit of detection for any compounds extracted from human urine by the optimized SPE method was 40 ng/mL and the limit of quantification was 100 ng/mL in the urine. The recovery rate of SPE was between 71 and 82% with a lower relative standard deviation than 2.35%.
authors.affiliation: 1Institute of Forensic Medicine, University of Pécs, H-7624 Pécs, Szigeti str. 12., Hungary.
authors.forename: Mátyás
authors.initials: M
authors.lastname: Mayer
authors.forename: András
authors.initials: A
authors.lastname: Benko
authors.forename: András
authors.initials: A
authors.lastname: Huszár
authors.forename: Katalin
authors.initials: K
authors.lastname: Sipos
authors.forename: Anikó
authors.initials: A
authors.lastname: Lajtai
authors.forename: Agnes
authors.initials: A
authors.lastname: Lakatos
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Porpáczy
mesh_terms: D000293:Adolescent; D000328:Adult; D000662:Amphetamines / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D016018:Least-Squares Analysis; D057230:Limit of Detection; D008297:Male; D008694:Methamphetamine / Q000031:analogs & derivatives* / Q000652:urine*; D011427:Propiophenones / Q000652:urine*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D055815:Young Adult
chemical_list: D000662:Amphetamines; D011427:Propiophenones; D008694:Methamphetamine; C585202:4-methylethcathinone; C578181:4-fluoromethcathinone