title: Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices.
abstract: The suitable optical properties of thiol-ene polymers combined with the ease of modifying their surface for the attachment of recognition molecules make them ideal candidates in many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in microfluidic thiol-ene chips. This work focuses on thiol-ene substrates featuring an excess of thiol groups at their surface. The thiol-ene stoichiometric composition can be varied to precisely control the number of surface thiol groups available for surface modification up to an average surface density of 136 ± 17 SH nm(-2). Biotin alkyne was patterned directly inside thiol-ene microchannels prior to conjugation with fluorescently labelled streptavidin. The surface bound conjugates were detected by evanescent wave-induced fluorescence (EWIF), demonstrating the success of the grafting procedure and its potential for biochip applications.
authors.affiliation: Department of Micro- and Nanotechnology, Technical University of Denmark, DK-2800 Kgs Lyngby, Denmark.
authors.forename: Josiane P
authors.initials: JP
authors.lastname: Lafleur
authors.forename: Radoslaw
authors.initials: R
authors.lastname: Kwapiszewski
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Jensen
authors.forename: Jörg P
authors.initials: JP
authors.lastname: Kutter
mesh_terms: D000480:Alkynes / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D046210:Microfluidic Analytical Techniques; D011108:Polymers / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000295:instrumentation; D019809:Streptavidin / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000480:Alkynes; D005456:Fluorescent Dyes; D011108:Polymers; D013438:Sulfhydryl Compounds; D001710:Biotin; D019809:Streptavidin