title: Culture of common carp (Cyprinus carpio)  with defined flesh quality for prevention of cardiovascular diseases using finishing feeding strategy.
abstract: OBJECTIVE
  Fish is the major source of n-3 polyunsaturated fatty acids (n-3 PUFA) which are well known to have positive effects in prevention of cardiovascular diseases. This study investigated the possibility to produce common carp with defined flesh quality using finishing feeding strategy and predict changes of fillet FA by a dilution model.
  
  
  METHODS
  During the 110-day experiment, fish were fed diets with two different vegetable oils (rapeseed/linseed blend, VO; olive oil, OO) only, or with a subsequent fish oil (FO) finishing treatment for 30 or 60 days. Fillet FA composition was measured and data were compared to the ones predicted by the dilution model.
  
  
  RESULTS
  The FO finishing treatment resulted in the higher percentage of SFA (from 19.1% to 23.6%; p<0.001), MUFA (from 46.8% to 51.9%; p<0.001), n-3 PUFA (from 3.6% to 7.4%; p<0.001) and lower n-6 PUFA (from 30.5% to 16.9%; p<0.001) and n-6/n-3 ratio (from 8.7 to 2.3; p<0.001) in groups previously fed the VO diet and in lower MUFA percentage (from 67% to 63%; p<0.001) and n-6/n-3 ratio (from 8.2 to 2.8; p<0.001) and higher n-3 PUFA percentage (from 1.5% to 4.5%; p<0.001) in group previously fed the OO diet. The dilution model gave a good prediction for fillet FA changes (slope of the regression line 0.97-1.00; R2 value of 0.992-0.996).
  
  
  CONCLUSIONS
  The finishing feeding strategy is suggested for production of common carp with a required flesh FA composition for purposes of special nutritional needs, especially for primary and secondary prevention of cardiovascular disease.
authors.affiliation: South Bohemian Research Centre of Aquaculture and Biodiversity, University of South Bohemia, Ceske Budejovice, Czech Republic. jmraz@frov.jcu.cz
authors.forename: Jan
authors.initials: J
authors.lastname: Mraz
authors.forename: Tomas
authors.initials: T
authors.lastname: Zajic
authors.forename: Jana
authors.initials: J
authors.lastname: Pickova
mesh_terms: D000821:Animal Feed; D000818:Animals; D017756:Aquaculture / Q000379:methods*; D002318:Cardiovascular Diseases / Q000178:diet therapy* / Q000517:prevention & control*; D002347:Carps / Q000254:growth & development / Q000378:metabolism*; D004281:Docosahexaenoic Acids / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000378:metabolism; D043371:Fatty Acids, Omega-6 / Q000378:metabolism; D005260:Female; D005395:Fish Oils / Q000378:metabolism; D006801:Humans; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism
chemical_list: D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D005395:Fish Oils; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid