title: A graphene and multienzyme functionalized carbon nanosphere-based electrochemical immunosensor for microcystin-LR detection.
abstract: A graphene-based immunosensor was fabricated for the detection of microcystin-LR using a horseradish peroxidase-carbon nanosphere-antibody system for signal amplification. Graphene and chitosan were used as immobilization materials to improve the electrochemical performance of the electrode. Signal amplification was achieved using multienzyme functionalized carbon nanosphere. This approach provided a linear range from 0.05 to 15 μgL(-1) microcystin-LR with a detection limit of 0.016 μgL(-1). Analytical detection of microcystin-LR in environmental water samples were demonstrated by comparing the results obtained using immunosensor and high-performance liquid chromatography. This electrochemical immunosensor showed good performance with high sensitivity, acceptable stability, repeatability and the potential for use in routine water quality monitoring for various toxins.
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China. zhaohuim@dlut.edu.cn
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhao
authors.forename: Junping
authors.initials: J
authors.lastname: Tian
authors.forename: Xie
authors.initials: X
authors.lastname: Quan
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D005898:Glass / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008387:Marine Toxins; D052998:Microcystins / Q000032:analysis* / Q000737:chemistry; D054118:Nanospheres / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D000906:Antibodies; D008387:Marine Toxins; D052998:Microcystins; D014867:Water; D002244:Carbon; D006108:Graphite; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; C057862:cyanoginosin LR