title: Imaging Fourier transform endospectroscopy for in vivo and in situ multispectral imaging.
abstract: We report the design and implementation of a multispectral imaging Fourier transform endospectroscopy (IFTES) system. The IFTES system employs a flexible fiber bundle catheter coupled to a home-built imaging Fourier transform spectroscope. The instrument enables the performance of non- or minimally invasive subsurface imaging and multispectral imaging at the cellular level in vivo and in situ. A maximum spectral resolution of 0.2 nm at 632.8 nm and a lateral resolution of 4.4 μm were proved. Preliminary results of a standard resolution target, ex-vivo small animal tissue, single wavelength laser, fluorescence solution, in-vivo mouse skin, microspheres mixture, and in-vivo transgenic mouse brain were given to demonstrate the potential of the technique.
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, 1037 Luoyu Rd., Wuhan 430074, China.
authors.forename: Hongming
authors.initials: H
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
authors.forename: Ling
authors.initials: L
authors.lastname: Fu
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology*; D057785:Catheters; D004724:Endoscopy / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D051379:Mice; D008822:Mice, Transgenic; D012867:Skin / Q000166:cytology*; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation*