title: Lateral shearing digital holographic imaging of small biological specimens.
abstract: A lateral shearing interferometer is used for direct holographic imaging of microorganisms. This is achieved by increasing the shear to be larger than the object size and results in a very simple and inexpensive common-path imaging device that can be easily coupled to the output of an inverted microscope. The shear is created by reflections from the front and back surface of a glass plate. Stability measurements show a standard deviation of the phase measurements of less than 1nm over 8 min. without any vibration compensation. The setup is applied to imaging both microorganisms in a microfluidic channel and red blood cells and reconstructions are presented.
authors.affiliation: Center of Medical Physics and Biomedical Engineering, Medical University of Vienna, Waehringer Guertel 18-20, 4L, 1090 Vienna, Austria.
authors.forename: Amardeep S G
authors.initials: AS
authors.lastname: Singh
authors.forename: Arun
authors.initials: A
authors.lastname: Anand
authors.forename: Rainer A
authors.initials: RA
authors.lastname: Leitgeb
authors.forename: Bahram
authors.initials: B
authors.lastname: Javidi
mesh_terms: D001783:Blood Flow Velocity / Q000502:physiology; D058948:Cell Tracking / Q000295:instrumentation*; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004912:Erythrocytes / Q000166:cytology* / Q000502:physiology*; D006696:Holography / Q000295:instrumentation*; D006801:Humans; D007368:Interferometry / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008853:Microscopy / Q000295:instrumentation*; D033081:Shear Strength / Q000502:physiology