title: Stability and in vitro antiproliferative activity of bioactive "Vitamin E" fortified parenteral lipid emulsions.
abstract: The objectives of this work were to engineer physically stable "Vitamin E" rich intravenous lipid emulsions and to evaluate their in vitro antiproliferative activity against MCF-7 (human mammary adenocarcinoma) and SW-620 (human colon adenocarcinoma) cell lines. Emulsions loaded with 70% vitamin E by total weight of the oil phase were stabilized with secondary emulsifiers and tested for their hemolytic effect and their plasma and electrolyte stability. Emulsions stabilized with sodium oleate and sodium deoxycholate were sensitive to electrolytes and exhibited significant hemolytic effect. On the other hand, addition of 2.5% poloxamer was found to stabilize the emulsions against electrolytes and physical stress, which was attributed to the steric effect of their polyoxyethylene (POE) head group. When tested for their antiproliferative effects, poloxamer-stabilized tocotrienol lipid emulsions were found to exhibit significantly higher anticancer activity than lipid emulsions enriched with tocopherol alone. The half maximal inhibitory concentrations (IC(50)) of tocotrienols lipid emulsions against MCF-7 and SW-620 were 14 and 12 μM, respectively, whereas the IC(50s) of tocopherol lipid emulsions were approximately 69 and 78 μM against MCF-7 and SW-620 cells, respectively.
authors.affiliation: Department of Basic Pharmaceutical Sciences, College of Pharmacy, University of Louisiana at Monroe, Monroe, LA, United States.
authors.forename: Alaadin
authors.initials: A
authors.lastname: Alayoubi
authors.forename: Shanthi
authors.initials: S
authors.lastname: Kanthala
authors.forename: Seetharama D
authors.initials: SD
authors.lastname: Satyanarayanajois
authors.forename: John F
authors.initials: JF
authors.lastname: Anderson
authors.forename: Paul W
authors.initials: PW
authors.lastname: Sylvester
authors.forename: Sami
authors.initials: S
authors.lastname: Nazzal
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004355:Drug Stability; D004573:Electrolytes / Q000494:pharmacology; D045703:Emulsifying Agents / Q000737:chemistry*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D010316:Particle Size; D020442:Poloxamer / Q000737:chemistry; D011817:Rabbits; D024505:Tocopherols / Q000737:chemistry; D024508:Tocotrienols / Q000737:chemistry; D014810:Vitamin E / Q000097:blood / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D004573:Electrolytes; D045703:Emulsifying Agents; D008055:Lipids; D024508:Tocotrienols; D020442:Poloxamer; D014810:Vitamin E; D024505:Tocopherols