title: cGMP-PDE3-cAMP signal pathway involved in the inhibitory effect of CNP on gastric motility in rat.
abstract: In the present study, we investigated the mechanism of C-type natriuretic peptide (CNP)-induced inhibitory effect on spontaneous contraction of gastric antral smooth muscle to clarify CNP-NPR-B/pGC-cGMP downstream signal transduction pathway using organ bath and ELISA methods in rat. CNP significantly reduced the amplitude of the spontaneous contraction and increased the contents of cGMP and cAMP in the gastric antral smooth muscle tissue. In the presence of IBMX, a non-selective phosphodiesterase (PDE) inhibitor, the inhibitory effect of CNP on spontaneous contraction was significantly suppressed; however, the production of cGMP but not cAMP was still increased by CNP. EHNA, a PDE2 inhibitor, did not affect both CNP-induced inhibition of the contraction and CNP-induced increase of cGMP and cAMP generations in gastric smooth muscle tissue, while milrinone, a PDE3 inhibitor, similar to IBMX, attenuated the CNP-induced inhibitory effect on spontaneous contraction and increased the content of cGMP but not cAMP. The results suggest that cGMP-PDE3-cAMP signal pathway is also involved in the CNP-induced inhibition of gastric motility in rat.
authors.affiliation: Department of Physiology, Yanbian University School of Medicine, Jilin, Yanji 133002, China.
authors.forename: Ying-Lan
authors.initials: YL
authors.lastname: Cai
authors.forename: Qian
authors.initials: Q
authors.lastname: Sun
authors.forename: Xu
authors.initials: X
authors.lastname: Huang
authors.forename: Jing-Zhi
authors.initials: JZ
authors.lastname: Jiang
authors.forename: Mo-Han
authors.initials: MH
authors.lastname: Zhang
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Piao
authors.forename: Zheng
authors.initials: Z
authors.lastname: Jin
authors.forename: Wen-Xie
authors.initials: WX
authors.lastname: Xu
mesh_terms: D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3 / Q000378:metabolism*; D005260:Female; D005769:Gastrointestinal Motility / Q000502:physiology*; D008297:Male; D020098:Natriuretic Peptide, C-Type / Q000502:physiology*; D051381:Rats; D015398:Signal Transduction
chemical_list: D020098:Natriuretic Peptide, C-Type; D000242:Cyclic AMP; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3; D006152:Cyclic GMP