title: Prooxidant actions of bisphenol A (BPA) phenoxyl radicals: implications to BPA-related oxidative stress and toxicity.
abstract: We investigated the prooxidant effects of bisphenol A (BPA) phenoxyl radicals in comparison with the phenoxyl radicals of 3-tert-butyl-4-hydroxyanisole (BHA), 2,6-di-tert-butyl-methylphenol (BHT) and 4-tert-butylphenol (TBP). The phenoxyl radicals, generated in situ by 1-electron oxidation of the corresponding phenol, were allowed to react with reduced nicotinamide adenine dinucleotide phosphate (NADPH) and rifampicin. The antioxidant activity of various phenols was examined based on the reduction of 2,2'-diphenyl-1-picrylhydrazyl radical (DPPH). It was found that the prooxidant activity of BPA phenoxyl radicals far exceeded those of BHA and BHT of phenoxyl radicals. Unlike Trolox, BPA showed minimal DPPH scavenging activity. The strong prooxidant properties of BPA phenoxyl radicals propelled us to study the markers of cellular oxidative stress in GT1-7 hypothalamic neurons exposed to BPA. It was observed that neuronal cells exposed to BPA had increased generation of intracellular peroxides and mitochondrial superoxide ([Formula: see text]). The formation of peroxides and [Formula: see text] were time- and dose-dependent and that co-incubation with N-acetyl-l-cysteine or Trolox greatly lowered their levels. The results of the present study are consistent with emerging evidence that human populations (non-institutionalized) having higher levels of urinary BPA also have increased levels of oxidative stress markers and are prone to higher risk of cardiovascular diseases, diabetes and abnormalities in hepatic enzymes.
authors.affiliation: Department of Environmental Toxicology and the Health Research Center, Southern University and A&M College, Baton Rouge, LA 70813, USA.
authors.forename: Sainath
authors.initials: S
authors.lastname: Babu
authors.forename: Satvika
authors.initials: S
authors.lastname: Uppu
authors.forename: Michelle O
authors.initials: MO
authors.lastname: Claville
authors.forename: Rao M
authors.initials: RM
authors.lastname: Uppu
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000737:chemistry / Q000633:toxicity*; D001713:Biphenyl Compounds / Q000737:chemistry; D002083:Butylated Hydroxyanisole / Q000737:chemistry / Q000633:toxicity; D002084:Butylated Hydroxytoluene; D018929:Cell Culture Techniques; D002460:Cell Line; D005609:Free Radicals / Q000737:chemistry / Q000633:toxicity*; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D015394:Molecular Structure; D009249:NADP / Q000737:chemistry; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D016877:Oxidants / Q000737:chemistry / Q000633:toxicity*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010545:Peroxides / Q000378:metabolism; D010636:Phenols / Q000737:chemistry / Q000633:toxicity*; D010851:Picrates / Q000737:chemistry; D012293:Rifampin / Q000737:chemistry; D013481:Superoxides / Q000378:metabolism; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D001713:Biphenyl Compounds; D002083:Butylated Hydroxyanisole; D002084:Butylated Hydroxytoluene; D005609:Free Radicals; D009249:NADP; D016877:Oxidants; D010545:Peroxides; D010636:Phenols; D010851:Picrates; D012293:Rifampin; D013481:Superoxides; C042329:phenoxy radical; C051362:3-tert-butyl-4-hydroxyanisole; C004931:1,1-diphenyl-2-picrylhydrazyl; C006780:bisphenol A; D000098826:Bisphenol A Compounds; C006862:butylphen