title: Biliary PAH metabolites, EROD activity and DNA damage in dab (Limanda limanda) from Seine Estuary (France).
abstract: The Seine Estuary is well known to be widely contaminated by organic pollutants and especially by polycyclic aromatic hydrocarbons (PAHs). Fish are known to metabolize PAHs, leading to different toxic effects at both cellular and sub-cellular levels. In this work, we studied the relationships between the 7-ethoxyresorufin-O-deethylase (EROD) activity in the liver, the level of DNA strand breaks in blood cells and the concentration of PAH metabolites in the bile of the sentinel flatfish species Limanda limanda. Muscle and liver samples were analysed for parent PAH levels. Female and male dabs of two size classes (juveniles and adults) were collected by trawling in two sites with different degrees of pollution during March and September 2005 and 2006. Significant effects of sex, age, site and season were demonstrated on EROD activity and the level of strand breaks. Parent PAH concentrations in dabs did not allow discriminating of the two sampling sites. However, for PAH metabolites, significant differences were observed with sites and seasons. Dabs collected at the mouth of the estuary appeared to be the most impacted when looking at the results obtained with the three selected markers. The significant correlations observed between the level of PAH metabolites and the level of DNA lesions showed the importance of a combined analysis of chemical and biochemical markers to correctly assess the contribution of chemical contamination to the toxic effects measured in situ in fish.
authors.affiliation: Oceanic and Continental Environments and Paleoenvironments, UMR 5805 CNRS, Laboratory of Physico- and Toxico-Chemistry of the Environment, Université de Bordeaux, 351 cours de la Libération, 33405, Talence Cedex, France.
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Dévier
authors.forename: Marie
authors.initials: M
authors.lastname: Le Dû-Lacoste
authors.forename: Farida
authors.initials: F
authors.lastname: Akcha
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Morin
authors.forename: Laurent
authors.initials: L
authors.lastname: Peluhet
authors.forename: Karyn
authors.initials: K
authors.lastname: Le Menach
authors.forename: Thierry
authors.initials: T
authors.lastname: Burgeot
authors.forename: Hélène
authors.initials: H
authors.lastname: Budzinski
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D004249:DNA Damage; D004784:Environmental Monitoring / Q000379:methods; D063366:Estuaries; D005260:Female; D005412:Flatfishes / Q000235:genetics* / Q000378:metabolism*; D005602:France; D008401:Gas Chromatography-Mass Spectrometry; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000187:drug effects / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity*; D012621:Seasons; D014877:Water Pollution, Chemical / Q000032:analysis*
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D019363:Cytochrome P-450 CYP1A1