title: Fluoroquinolone photodegradation influences specific basophil activation.
abstract: Fluoroquinolones (FQs) are photoreactive drugs, but it is not known whether laboratory light exposure can influence the induction of photoproducts and modify in vitro test results. The basophil activation test (BAT) has proven to be useful for evaluating immunoglobulin E (IgE)-mediated hypersensitivity to FQs, with a higher percentage of positive responders with ciprofloxacin (CIP) than with moxifloxacin (MOX). We studied the effect of laboratory light on CIP and MOX degradation, and drug-protein conjugate formation, and its influence on the BAT for evaluating IgE-mediated hypersensitivity to FQs. The results showed an important decrease in fluorescence emission intensity under light compared to dark conditions for MOX, and that BAT positivity was lower in light (17.9%) than in dark (35.7%) conditions. No changes were found for CIP in either fluorescence emission intensity or BAT results (46.4% in both conditions). We can conclude that light exposure is a critical factor in BAT results when photolabile drugs like MOX are used. Therefore, light is important when interpreting in vitro results.
authors.affiliation: Research Laboratory, Carlos Haya Hospital, Malaga, Spain.
authors.forename: Cristobalina
authors.initials: C
authors.lastname: Mayorga
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Andreu
authors.forename: Ana
authors.initials: A
authors.lastname: Aranda
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Doña
authors.forename: M Isabel
authors.initials: MI
authors.lastname: Montañez
authors.forename: Natalia
authors.initials: N
authors.lastname: Blanca-Lopez
authors.forename: Adriana
authors.initials: A
authors.lastname: Ariza
authors.forename: Edurne
authors.initials: E
authors.lastname: Nuin
authors.forename: Miguel
authors.initials: M
authors.lastname: Blanca
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Miranda
authors.forename: M Jose
authors.initials: MJ
authors.lastname: Torres
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001372:Aza Compounds / Q000737:chemistry; D001491:Basophils / Q000276:immunology*; D002939:Ciprofloxacin / Q000737:chemistry; D024841:Fluoroquinolones / Q000737:chemistry*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology; D008027:Light; D008875:Middle Aged; D000077266:Moxifloxacin; D010782:Photolysis; D011804:Quinolines / Q000737:chemistry; D055815:Young Adult
chemical_list: D001372:Aza Compounds; D024841:Fluoroquinolones; D011804:Quinolines; D007073:Immunoglobulin E; D002939:Ciprofloxacin; D000077266:Moxifloxacin