title: [Study on hepatitis C virus genotypes in Yantai district, Shandong province].
abstract: OBJECTIVE
  To understand the hepatitis C virus (HCV) genotype distribution in Yantai district of Shandong province, and to explore whether the HCV genotypes was relevant to the injure of liver through the index of liver function.
  
  
  METHODS
  Using specific PCR primers to amplify the HCV RNA 5' UTR/NS5B,then PCR products were sequenced by genetic analyzer. The genotypes were identified by alignment to the GenBank reference sequences and construction the phylogenetic tree of 5' UTR.
  
  
  RESULTS
  Among 9 unpaid blood donors we detected two kinds of genotypes of 1b and 3a, respectively, 8 cases (88.9%) and 1 case (11.1%). Among 33 cases of hepatitis C patients we detected the 1b, 2a and 6a the three kinds of genotypes, respectively, 22 (66.7%), 10 (30.3%) and 1 (3.03%) cases. Subtype 1b is the advantage of popular genotype in HCV carriers from Yantai district, and the distribution of 1b was no significant difference in the different population (chi2 = 0.796, P = 0.373); The difference of indicator of liver damage in the different genotypes of subjects were significant (P < 0.05), the mean of ALT, AST of 2a-subtype carriers was significantly higher than the 1b-subtype population.
  
  
  CONCLUSIONS
  The genetic diversity of HCV in Shandong Yantai district was presented. The main genotypes were 1b-subtype, and 3a and 6a-subtype was detected firstly. The genotype of HCV were relevant to the liver damage indicators, 2a-subtype hepatitis C virus infection in the liver cells may play an important role in the disease process.
authors.affiliation: Qingdao University Medical College, Qingdao 266021, China.
authors.forename: Guo-Ying
authors.initials: GY
authors.lastname: Zhang
authors.forename: Sheng-Bo
authors.initials: SB
authors.lastname: Pu
authors.forename: Xue-Ying
authors.initials: XY
authors.lastname: Yu
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Shi
authors.forename: Ai-Ling
authors.initials: AL
authors.lastname: Xu
authors.forename: Zhen-Hua
authors.initials: ZH
authors.lastname: Guo
authors.forename: Ming-Gang
authors.initials: MG
authors.lastname: Qiao
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Yong-Zheng
authors.initials: YZ
authors.lastname: Jing
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002681:China / Q000453:epidemiology; D005260:Female; D005838:Genotype; D016174:Hepacivirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006526:Hepatitis C / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D055815:Young Adult