title: Amyloidogenic peptides of yeast cell wall glucantransferase Bgl2p as a model for the investigation of its pH-dependent fibril formation.
abstract: The pH-dependence of the ability of Bgl2p to form fibrils was studied using synthetic peptides with potential amyloidogenic determinants (PADs) predicted in the Bgl2p sequence. Three PADs, FTIFVGV, SWNVLVA and NAFS, were selected on the basis of combination of computational algorithms. Peptides AEGFTIFVGV, VDSWNVLVAG and VMANAFSYWQ, containing these PADs, were synthesized. It was demonstrated that these peptides had an ability to fibrillate at pH values from 3.2 to 5.0. The PAD-containing peptides, except for VDSWNVLVAG, could fibrillate also at pH values from pH 5.0 to 7.6. We supposed that the ability of Bgl2p to form fibrils most likely depended on the coordination of fibrillation activity of the PAD-containing areas and Bgl2p could fibrillate at mild acid and neutral pH values and lose the ability to fibrillate with the increasing of pH values. It was demonstrated that Bgl2p was able to fibrillate at pH value 5.0, to form fibrils of various morphology at neutral pH values and lost the fibrillation ability at pH value 7.6. The results obtained allowed us to suggest a new simple approach for the isolation of Bgl2p from Saccharomyces cerevisiae cell wall.
authors.affiliation: Department of Molecular Biology, Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: Evgeny E
authors.initials: EE
authors.lastname: Bezsonov
authors.forename: Minna
authors.initials: M
authors.lastname: Groenning
authors.forename: Oxana V
authors.initials: OV
authors.lastname: Galzitskaya
authors.forename: Anton A
authors.initials: AA
authors.lastname: Gorkovskii
authors.forename: Gennady V
authors.initials: GV
authors.lastname: Semisotnov
authors.forename: Irina O
authors.initials: IO
authors.lastname: Selyakh
authors.forename: Rustam H
authors.initials: RH
authors.lastname: Ziganshin
authors.forename: Valentina V
authors.initials: VV
authors.lastname: Rekstina
authors.forename: Irina B
authors.initials: IB
authors.lastname: Kudryashova
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Kuznetsov
authors.forename: Igor S
authors.initials: IS
authors.lastname: Kulaev
authors.forename: Tatyana S
authors.initials: TS
authors.lastname: Kalebina
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D002473:Cell Wall / Q000737:chemistry / Q000201:enzymology; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000737:chemistry / Q000378:metabolism*; D016695:Glycosyltransferases / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000682:Amyloid; D010455:Peptides; D029701:Saccharomyces cerevisiae Proteins; D016695:Glycosyltransferases; C080389:BGL2 protein, S cerevisiae; D004693:Glucan Endo-1,3-beta-D-Glucosidase