title: Simultaneous measurement of neural spike recordings and multi-photon calcium imaging in neuroblastoma cells.
abstract: This paper proposes the design and implementation of a micro-electrode array (MEA) for neuroblastoma cell culturing. It also explains the implementation of a  multi-photon microscope (MPM) customized for neuroblastoma cell excitation and imaging under ambient light. Electrical signal and fluorescence images were simultaneously acquired from the neuroblastoma cells on the MEA. MPM calcium images of the cultured neuroblastoma cell on the MEA are presented and also the neural activity was acquired through the MEA recording. A calcium green-1 (CG-1) dextran conjugate of 10,000 D molecular weight was used in this experiment for calcium imaging. This study also evaluated the calcium oscillations and neural spike recording of neuroblastoma cells in an epileptic condition. Based on our observation of neural spikes in neuroblastoma cells with our proposed imaging modality, we report that neuroblastoma cells can be an important model for epileptic activity studies.
authors.affiliation: School of Electrical Engineering and Computer Science, Kyungpook National University, Buk-gu, Daegu, Korea. shkim@ee.knu.ac.kr
authors.forename: Suhwan
authors.initials: S
authors.lastname: Kim
authors.forename: Unsang
authors.initials: U
authors.lastname: Jung
authors.forename: Juyeong
authors.initials: J
authors.lastname: Baek
authors.forename: Shinwon
authors.initials: S
authors.lastname: Kang
authors.forename: Jeehyun
authors.initials: J
authors.lastname: Kim
mesh_terms: D000200:Action Potentials; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D045744:Cell Line, Tumor; D006801:Humans; D008856:Microscopy, Fluorescence; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D009474:Neurons / Q000502:physiology*
chemical_list: D002118:Calcium