title: Structural characterization of supramolecular assemblies by ¹³C spin dilution and 3D solid-state NMR.
abstract: (13)C spin diluted protein samples can be produced using [1-(13)C] and [2-(13)C]-glucose (Glc) carbon sources in the bacterial growth medium. The (13)C spin dilution results in favorable (13)C spectral resolution and polarization transfer behavior. We recently reported the combined use of [1-(13)C]- and [2-(13)C]-Glc labeling to facilitate the structural analysis of insoluble and non-crystalline biological systems by solid-state NMR (ssNMR), including sequential assignment, detection of long-range contacts and structure determination of macromolecular assemblies. In solution NMR the beneficial properties of sparsely labeled samples using [2-(13)C]-glycerol ((13)C labeled Cα sites on a (12)C diluted background) have recently been exploited to provide a bi-directional assignment method (Takeuchi et al. in J Biomol NMR 49(1):17-26, 2011 ). Inspired by this approach and our own recent results using [2-(13)C]-Glc as carbon sources for the simplification of ssNMR spectra, we present a strategy for a bi-directional sequential assignment of solid-state NMR resonances and additionally the detection of long-range contacts using the combination of (13)C spin dilution and 3D NMR spectroscopy. We illustrate our results with the sequential assignment and the collection of distance restraints on an insoluble and non-crystalline supramolecular assembly, the Salmonella typhimurium type III secretion system needle.
authors.affiliation: Department of NMR-based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Habenstein
authors.forename: Antoine
authors.initials: A
authors.lastname: Loquet
authors.forename: Karin
authors.initials: K
authors.lastname: Giller
authors.forename: Stefan
authors.initials: S
authors.lastname: Becker
authors.forename: Adam
authors.initials: A
authors.lastname: Lange
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D058947:Bacterial Secretion Systems; D002247:Carbon Isotopes / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D012486:Salmonella typhimurium / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D058947:Bacterial Secretion Systems; D002247:Carbon Isotopes