title: KIF14 negatively regulates Rap1a-Radil signaling during breast cancer progression.
abstract: The small GTPase Rap1 regulates inside-out integrin activation and thereby influences cell adhesion, migration, and polarity. Several Rap1 effectors have been described to mediate the cellular effects of Rap1 in a context-dependent manner. Radil is emerging as an important Rap effector implicated in cell spreading and migration, but the molecular mechanisms underlying its functions are unclear. We report here that the kinesin KIF14 associates with the PDZ domain of Radil and negatively regulates Rap1-mediated inside-out integrin activation by tethering Radil on microtubules. The depletion of KIF14 led to increased cell spreading, altered focal adhesion dynamics, and inhibition of cell migration and invasion. We also show that Radil is important for breast cancer cell proliferation and for metastasis in mice. Our findings provide evidence that the concurrent up-regulation of Rap1 activity and increased KIF14 levels in several cancers is needed to reach optimal levels of Rap1-Radil signaling, integrin activation, and cell-matrix adhesiveness required for tumor progression.
authors.affiliation: Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, Faculty of Medicine, University of Toronto, Toronto, Ontario M5S 1A1, Canada.
authors.forename: Syed M
authors.initials: SM
authors.lastname: Ahmed
authors.forename: Brigitte L
authors.initials: BL
authors.lastname: Thériault
authors.forename: Maruti
authors.initials: M
authors.lastname: Uppalapati
authors.forename: Catherine W N
authors.initials: CW
authors.lastname: Chiu
authors.forename: Brenda L
authors.initials: BL
authors.lastname: Gallie
authors.forename: Sachdev S
authors.initials: SS
authors.lastname: Sidhu
authors.forename: Stéphane
authors.initials: S
authors.lastname: Angers
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000556:secondary; D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D016547:Kinesins / Q000378:metabolism*; D008175:Lung Neoplasms / Q000378:metabolism / Q000556:secondary; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D015513:Oncogene Proteins / Q000378:metabolism*; D015398:Signal Transduction; D014183:Transplantation, Heterologous; D020827:rap1 GTP-Binding Proteins / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D015513:Oncogene Proteins; C548041:RADIL protein, human; C430803:RAP1A protein, human; C502598:KIF14 protein, human; D016547:Kinesins; D020827:rap1 GTP-Binding Proteins