title: Preparation of coaxial-electrospun poly[bis(p-methylphenoxy)]phosphazene nanofiber membrane for enzyme immobilization.
abstract: A core/sheath nanofiber membrane with poly[bis(p-methylphenoxy)]phospha-zene (PMPPh) as the sheath and easily spinnable polyacrylonitrile (PAN) as the core was prepared via a coaxial electrospinning process. Field-emission scanning electron microscopy and transmission electron microscopy were used to characterize the morphology of the nanofiber membrane. It was found that the concentration of the PAN spinning solution and the ratio of the core/sheath solution flow rates played a decisive role in the coaxial electrospinning process. In addition, the stabilized core/sheath PMPPh nanofiber membrane was investigated as a support for enzyme immobilization because of its excellent biocompatibility, high surface/volume ratio, and large porosity. Lipase from Candida rugosa was immobilized on the nanofiber membrane by adsorption. The properties of the immobilized lipase on the polyphosphazene nanofiber membrane were studied and compared with those of a PAN nanofiber membrane. The results showed that the adsorption capacity (20.4 ± 2.7 mg/g) and activity retention (63.7%) of the immobilized lipase on the polyphosphazene nanofiber membrane were higher than those on the PAN membrane.
authors.affiliation: Key Laboratory of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi 214122, China. wshugen@yahoo.com.cn
authors.forename: Shu-Gen
authors.initials: SG
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Jiang
authors.forename: Peng-Cheng
authors.initials: PC
authors.lastname: Chen
authors.forename: An-Guo
authors.initials: AG
authors.lastname: Yu
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Huang
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D002175:Candida / Q000201:enzymology; D004789:Enzyme Activation; D004800:Enzymes, Immobilized; D008049:Lipase / Q000737:chemistry / Q000378:metabolism; D008567:Membranes, Artificial; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D009943:Organophosphorus Compounds / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D013841:Thiazepines
chemical_list: D000180:Acrylic Resins; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D009943:Organophosphorus Compounds; D011108:Polymers; D013841:Thiazepines; C108974:poly(phosphazene); C050504:tianeptine; C010504:polyacrylonitrile; D008049:Lipase