title: Bone regeneration with rabbit bone marrow-derived mesenchymal stem cells and bone graft materials.
abstract: OBJECTIVE
  This study compared the bone regeneration response of different bone graft materials inside canals within anodized titanium implants in cortical and cancellous bone.
  
  
  METHODS
  Upper and lower transverse canals were created in anodic oxidized-surface titanium implants to serve as sites for cortical and cancellous bone regeneration, respectively. The canals were filled with bone graft materials--rabbit bone marrow--derived mesenchymal stem cells and platelet-rich plasma, xenograft, or alloplast (micromacroporous biphasic calcium phosphate)-or left empty (as a control). Eighty implants were surgically placed into the tibiae of 20 New Zealand white rabbits. After 4 and 12 weeks of healing, histomorphometric analysis was performed to measure the newly formed bone areas (NBs) inside the canals.
  
  
  RESULTS
  Inside the upper canals, the bone graft groups provided significantly higher NBs than the control (no graft). However, there was no significant difference in NBs between the bone graft groups. Inside the lower canals, no significant difference in NBs was shown among the all groups. The NBs inside the upper canals were significantly greater than those inside the lower canals in all groups after 4 and 12 weeks, respectively.
  
  
  CONCLUSIONS
  In the cortical bone, there was significant difference in bone regeneration between the control and the bone graft groups. However, there was no significant difference among the bone graft groups in cortical and cancellous bone regeneration. There was significant difference in bone regeneration between the cortical and cancellous bone regions in the all groups using the titanium canal model.
authors.affiliation: Department of Prosthodontics and Dental Research Institute, School of Dentistry, Seoul National University, and Kangnam Severance Dental Hospital, Seoul, South Korea.
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Lee
authors.forename: Seong-Joo
authors.initials: SJ
authors.lastname: Heo
authors.forename: Jai-Young
authors.initials: JY
authors.lastname: Koak
authors.forename: Seong-Kyun
authors.initials: SK
authors.lastname: Kim
authors.forename: Chong-Hyun
authors.initials: CH
authors.lastname: Han
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D001861:Bone Regeneration; D016025:Bone Transplantation; D002516:Ceramics / Q000627:therapeutic use*; D003772:Dental Pins; D006882:Hydroxyapatites / Q000008:administration & dosage*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D053657:Platelet-Rich Plasma; D011817:Rabbits; D013977:Tibia; D013997:Time Factors; D014025:Titanium; D014183:Transplantation, Heterologous; D014945:Wound Healing
chemical_list: D006882:Hydroxyapatites; C074950:hydroxyapatite-beta tricalcium phosphate; D014025:Titanium