title: Stromal cell-derived factor-1 enhances wound healing through recruiting bone marrow-derived mesenchymal stem cells to the wound area and promoting neovascularization.
abstract: Stromal cell-derived factor-1 (SDF-1) is a potent chemokine for bone marrow-derived stromal stem cells (BMSCs) that express CXCR4, the receptor for SDF-1. SDF-1 is considered to play an important role in the trafficking of BMSCs. We investigated the contribution of SDF-1 to the recruitment of BMSCs to the wound area and its promotion of wound repair and neovascularization. BMSCs were pretreated with or without anti-CXCR4 blocking antibody and combined with CM-DiI label, and injected via the tail vein into mice with full-thickness skin wounds on the dorsum. Simultaneously, anti-SDF-1 antibody was injected into local wounds in another group of mice. The results show that blockade of CXCR4 on either infused BMSCs or SDF-1 in the host wounds (1) dramatically impaired the number of infused BMSCs being recruited to the injured tissue, (2) reduced the expression of growth factors involved in the repair of injured tissue such as vascular endothelial growth factor, basic fibroblast growth factor and transforming growth factor beta 1, (3) decreased the resultant neovascularization, and (4) retarded wound healing. Taken together, the findings indicate that the SDF-1/CXCR4 signal pathway facilitates wound healing through augmenting BMSC recruitment to wound tissues, responsive secretion of growth factors by BMSCs and neovascularization in the wound area.
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Xu
authors.forename: Fangqiang
authors.initials: F
authors.lastname: Zhu
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.forename: Dengfen
authors.initials: D
authors.lastname: Zeng
authors.forename: Donglin
authors.initials: D
authors.lastname: Luo
authors.forename: Guodong
authors.initials: G
authors.lastname: Liu
authors.forename: Wenhui
authors.initials: W
authors.lastname: Cui
authors.forename: Shali
authors.initials: S
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.forename: Wei
authors.initials: W
authors.lastname: Xing
authors.forename: Huaping
authors.initials: H
authors.lastname: Liang
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Fu
authors.forename: Jianxin
authors.initials: J
authors.lastname: Jiang
authors.forename: Hong
authors.initials: H
authors.lastname: Huang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D054377:Chemokine CXCL12 / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D018919:Neovascularization, Physiologic / Q000502:physiology; D011897:Random Allocation; D033581:Stem Cell Transplantation; D014945:Wound Healing / Q000502:physiology*
chemical_list: D054377:Chemokine CXCL12