title: Combination therapy of dexamethasone with epigallocatechin enhances tibiotarsal bone articulation and modulates oxidative status correlates with cartilage cytokines expression in the early phase of experimental arthritis.
abstract: The inclusion of antioxidant for the treatment of arthritis, especially under the therapy with immunosuppressant, is motivated because antioxidant plays an essential role in disease progression and moreover, immunosuppressive treatment suffers redox homeostasis balance of the organism. The aim of the present study was to evaluate the enhancement of anti-arthritic effect of dexamethasone in combination with epigallocatechin on the progression of adjuvant-induced arthritis in rats. Adjuvant arthritic rats were treated with dexamethasone (0.2mg/kg), epigallocatechin (100mg/kg) and combination of dexamethasone (0.1mg/kg) with epigallocatechin (100mg/kg) daily for a period of 28 days. Paw swelling changes, estimation of serum albumin level, alteration of bone mineral density, histopathological, and radiographical analysis were assessed to evaluate the anti-arthritic effect. Lipid peroxidation and antioxidant enzyme activities in joint tissue homogenate were performed along with the expression of different pro-inflammatory cartilage cytokines like TNF-α and IL-6. Dexamethasone and epigallocatechin combination potentiated both the antiarthritic (decrease of hind paw volume) and the antioxidant effect (lipid peroxidation, superoxide dismutase, glutathione reductase and catalase). In combination with dexamethasone, epigallocatechin markedly potentiated the beneficial effect of dexamethasone which resulted in more significant increment of serum albumin and bone mineral density. Improvement of anti-arthritic effect of combination therapy was supported by histopathological, radiographical alterations, and attenuation of over-expression of cartilage cytokines. Epigallocatechin act as potent antioxidant and combined administration of dexamethasone with epigallocatechin increased the anti-arthritic efficacy of basal dexamethasone therapy and suppressed the development phase of arthritic progression in rats.
authors.affiliation: College of Pharmaceutical Technology, NSHM Knowledge Campus, 124 B L Saha Road, Kolkata, West Bengal 700053, India. souvikroy35@gmail.com
authors.forename: Souvik
authors.initials: S
authors.lastname: Roy
authors.forename: Santanu
authors.initials: S
authors.lastname: Sannigrahi
authors.forename: Balaram
authors.initials: B
authors.lastname: Ghosh
authors.forename: Priyanka
authors.initials: P
authors.lastname: Pusp
authors.forename: Tathagata
authors.initials: T
authors.lastname: Roy
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D015519:Bone Density / Q000187:drug effects; D050281:Bones of Lower Extremity / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D002356:Cartilage / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D016207:Cytokines / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology* / Q000627:therapeutic use; D004347:Drug Interactions; D005786:Gene Expression Regulation / Q000187:drug effects; D006614:Hindlimb / Q000187:drug effects / Q000473:pathology; D007375:Interleukin-1 / Q000378:metabolism; D007596:Joints / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016207:Cytokines; D007375:Interleukin-1; D012709:Serum Albumin; D014409:Tumor Necrosis Factor-alpha; D003907:Dexamethasone; D002392:Catechin; C057580:gallocatechol