title: Polymorphisms in thymic stromal lymphopoietin gene demonstrate a gender and nasal polyposis-dependent association with chronic rhinosinusitis.
abstract: BACKGROUND
  Recent studies have suggested that thymic stromal lymphopoietin (TSLP), a key cytokine involved in the dendritic cell-mediated activation of Th2-mediated inflammatory responses, is significantly increased in nasal polyps from atopic individuals. Our objective was therefore to explore firstly any associations between single nucleotide polymorphisms (SNPs) in and around the TSLP gene and development of chronic rhinosinusitis (CRS; with (CRSwNP) or without (CRSsNP) nasal polyps and, and secondly the influence of nasal polyposis and gender.
  
  
  METHODS
  A population-based case-control association analysis was performed in a Han Chinese cohort. DNA extracted from peripheral blood leukocytes from 638 subjects with CRS (306 CRSwNP and 332 CRSsNP) and 325 healthy controls was assessed for 11 SNPs in and around TSLP gene, selected from the Chinese HapMap genotyping dataset. Genetic association tests were performed using the Haploview and STATA software package.
  
  
  RESULTS
  Single-locus analysis of CRS risk, showed no significant differences in allele or genotype frequencies between CRS subjects and controls. Stratified analyses of association between the selected SNPs and CRS adjusted for gender demonstrated that rs13156068 (CC genotype: P=0.010, OR=0.289) and rs764917 (CC genotype: P=0.040, OR=0.509) were significantly protective against CRS, whereas rs6886755 (GT genotype: P=0.040, OR=0.509) presented a risk among females. In contrast, rs764917 (CA genotype: P=0.033, OR=1.553) presented risk for CRS in males. Furthermore, SNPs rs252706 (AA genotype: P=0.012, OR=0.552) and rs764917 (CA genotype: P=0.001, OR=0.182) displayed protective roles among CRSwNP, but not CRSsNP, subjects.
  
  
  CONCLUSIONS
  This study suggests that SNPs in TSLP gene may exert a gender and/or nasal polyposis-dependent risk for development of CRS in Chinese subjects.
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Demin
authors.initials: D
authors.lastname: Han
authors.forename: Luo
authors.initials: L
authors.lastname: Zhang
authors.forename: Claus
authors.initials: C
authors.lastname: Bachert
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D044466:Asian People; D002681:China; D002908:Chronic Disease; D016207:Cytokines / Q000235:genetics*; D004171:Diploidy; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000150:complications* / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012220:Rhinitis / Q000150:complications* / Q000235:genetics*; D012737:Sex Factors; D012852:Sinusitis / Q000150:complications* / Q000235:genetics*; D055815:Young Adult; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D016207:Cytokines; D000094632:Thymic Stromal Lymphopoietin