title: Acidic nucleoplasmic DNA-binding protein (And-1) controls chromosome congression by regulating the assembly of centromere protein A (CENP-A) at centromeres.
abstract: The centromere is an epigenetically designated chromatin domain that is essential for the accurate segregation of chromosomes during mitosis. The incorporation of centromere protein A (CENP-A) into chromatin is fundamental in defining the centromeric loci. Newly synthesized CENP-A is loaded at centromeres in early G(1) phase by the CENP-A-specific histone chaperone Holliday junction recognition protein (HJURP) coupled with other chromatin assembly factors. However, it is unknown whether there are additional HJURP-interacting factor(s) involving in this process. Here we identify acidic nucleoplasmic DNA-binding protein 1 (And-1) as a new factor that is required for the assembly of CENP-A nucleosomes. And-1 interacts with both CENP-A and HJURP in a prenucleosomal complex, and the association of And-1 with CENP-A is increased during the cell cycle transition from mitosis to G(1) phase. And-1 down-regulation significantly compromises chromosome congression and the deposition of HJURP-CENP-A complexes at centromeres. Consistently, overexpression of And-1 enhances the assembly of CENP-A at centromeres. We conclude that And-1 is an important factor that functions together with HJURP to facilitate the cell cycle-specific recruitment of CENP-A to centromeres.
authors.affiliation: Department of Biochemistry and Molecular Biology, The George Washington University Medical School, Washington, D. C. 20037, USA.
authors.forename: Aimee
authors.initials: A
authors.lastname: Jaramillo-Lambert
authors.forename: Jing
authors.initials: J
authors.lastname: Hao
authors.forename: Haijie
authors.initials: H
authors.lastname: Xiao
authors.forename: Yongming
authors.initials: Y
authors.lastname: Li
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Han
authors.forename: Wenge
authors.initials: W
authors.lastname: Zhu
mesh_terms: D001324:Autoantigens / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D002503:Centromere / Q000737:chemistry / Q000378:metabolism*; D000076247:Centromere Protein A; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics* / Q000378:metabolism; D020090:Chromosome Segregation; D002877:Chromosomes, Human / Q000737:chemistry / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases; D008938:Mitosis; D011485:Protein Binding; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D001324:Autoantigens; C000619700:CENPA protein, human; D000076247:Centromere Protein A; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; C524371:HJURP protein, human; D034741:RNA, Small Interfering; C544202:WDHD1 protein, human; D008156:Luciferases