title: Direct bioanalytical sample injection with 2D LC-MS.
abstract: New analytical platforms have been developed in response to the need for attaining increased peak capacity for multicomponent complex analysis with higher sensitivity and characterization of the analytes, and high-throughput capabilities. This review outlines the fundamental principles of target and comprehensive 2D LC method development and encompasses applications of LC-LC and LC × LC coupled to MS in bioanalysis using a variety of online analytical procedures. It also provides a rationale for the usage of the most employed mass analyzers and ionization sources on these platforms.
authors.affiliation: Departamento de Química, Universidade Federal de São Carlos, Caixa Postal 676, 13565-905 São Carlos - SP, Brazil.
authors.forename: Nm
authors.initials: N
authors.lastname: Cassiano
authors.forename: Jc
authors.initials: J
authors.lastname: Barreiro
authors.forename: Rv
authors.initials: R
authors.lastname: Oliveira
authors.forename: Qb
authors.initials: Q
authors.lastname: Cass
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*
chemical_list: D020543:Proteome