title: Bacterial diversity in the saliva of patients with different oral hygiene indexes.
abstract: The objective of the present study was to evaluate the bacterial diversity in the saliva of patients with different oral hygiene indexes using of two 16S rRNA gene libraries. Each library was composed of samples from patients with different averages of the differentiated Silness-Löe biofilm index: the first library (A) with an index between 1.0 and 3.0 (considered a high index) and the second library (B) between 0 and 0.5 (considered a low index). Saliva DNA was extracted and the 16S rRNA gene was amplified and cloned. The obtained sequences were compared with those stored at NCBI and RDP GenBank. The saliva of patients with high index presented five known genera - Streptococcus, Granulicatella, Gemella, Veillonella and Peptostreptococcus - and 33.3% of nonculturable bacteria grouped into 23 operational taxonomic units (OTUs). The saliva of patients with low index differed significantly from the first library (p=0.000) and was composed of 42 OTUs distributed into 11 known genera - Streptococcus, Granulicatella, Gemella, Veillonella, Oribacterium, Haemophilus, Escherichia, Neisseria, Prevotella, Capnocytophaga, Actinomyces - including 24.87% of nonculturable bacteria. It was possible to conclude that there is greater bacterial diversity in the saliva of patients with low dental plaque in relation to patients with high dental plaque.
authors.affiliation: Multidisciplinary Support Center, Federal University of Amazonas, Manaus, AM, Brazil. juvpereira@ufam.edu.br
authors.forename: Juliana Vianna
authors.initials: JV
authors.lastname: Pereira
authors.forename: Luciana
authors.initials: L
authors.lastname: Leomil
authors.forename: Fabíola
authors.initials: F
authors.lastname: Rodrigues-Albuquerque
authors.forename: José Odair
authors.initials: JO
authors.lastname: Pereira
authors.forename: Spartaco
authors.initials: S
authors.lastname: Astolfi-Filho
mesh_terms: D000190:Actinomyces / Q000145:classification; D000328:Adult; D000368:Aged; D001419:Bacteria / Q000145:classification*; D018441:Biofilms / Q000145:classification*; D002206:Capnocytophaga / Q000145:classification; D056568:Carnobacteriaceae / Q000145:classification; D004925:Escherichia / Q000145:classification; D005260:Female; D058834:Gemella / Q000145:classification; D015723:Gene Library; D006190:Haemophilus / Q000145:classification; D006801:Humans; D008297:Male; D064307:Microbiota; D008875:Middle Aged; D009343:Neisseria / Q000145:classification; D009911:Oral Hygiene Index; D010462:Peptostreptococcus / Q000145:classification; D010512:Periodontal Index; D018720:Prevotella / Q000145:classification; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012463:Saliva / Q000382:microbiology*; D013291:Streptococcus / Q000145:classification; D014678:Veillonella / Q000145:classification; D055815:Young Adult
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S