title: Knockdown of UCHL5IP causes abnormalities in γ-tubulin localisation, spindle organisation and chromosome alignment in mouse oocyte meiotic maturation.
abstract: UCHL5IP is one of the subunits of the haus complex, which is important for microtubule generation, spindle bipolarity and accurate chromosome segregation in Drosophila and human mitotic cells. In this study, the expression and localisation of UCHL5IP were explored, as well as its functions in mouse oocyte meiotic maturation. The results showed that the UCHL5IP protein level was consistent during oocyte maturation and it was localised to the meiotic spindle in MI and MII stages. Knockdown of UCHL5IP led to spindle defects, chromosome misalignment and disruption of γ-tubulin localisation in the spindle poles. These results suggest that UCHL5IP plays critical roles in spindle formation during mouse oocyte meiotic maturation.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Ya-Peng
authors.initials: YP
authors.lastname: Wang
authors.forename: Shu-Tao
authors.initials: ST
authors.lastname: Qi
authors.forename: Yanchang
authors.initials: Y
authors.lastname: Wei
authors.forename: Zhao-Jia
authors.initials: ZJ
authors.lastname: Ge
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Hou
authors.forename: Ying-Chun
authors.initials: YC
authors.lastname: Ouyang
authors.forename: Heide
authors.initials: H
authors.lastname: Schatten
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Zhao
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Sun
mesh_terms: D000818:Animals; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D059471:In Vitro Oocyte Maturation Techniques; D008540:Meiosis; D008677:Metaphase; D051379:Mice; D008813:Mice, Inbred ICR; D008845:Microinjections; D018613:Microscopy, Confocal; D008869:Microtubule-Associated Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D060172:Morpholinos; D020320:Oligoribonucleotides, Antisense; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D009866:Oogenesis; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D021381:Protein Transport; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D034622:RNA Interference; D008941:Spindle Apparatus / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism*; D000097869:Polo-Like Kinase 1
chemical_list: D018797:Cell Cycle Proteins; D008869:Microtubule-Associated Proteins; D060172:Morpholinos; D020320:Oligoribonucleotides, Antisense; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D014404:Tubulin; D000097869:Polo-Like Kinase 1; C583407:TUBG1 protein, mouse; C583406:UCHL5IP protein, mouse