title: IGF-1 alters the human parietal pleural electrochemical profile by inhibiting ion trans-cellular transportation after interaction with its receptor.
abstract: OBJECTIVE
  The effect of IGF-1 in the human pleural permeability and the underlying mechanisms involved were investigated.
  
  
  METHODS
  Specimens from thoracic surgical patients were mounted in Ussing chambers. Solutions containing IGF-1 (1 nM-100 nM) and IGF-1 Receptor Inhibitor (1 μΜ), amiloride 10 μM (Na(+) channel blocker) and ouabain 1 mM (Na(+)-K(+) pump inhibitor) were used in order to investigate receptor and ion transporter involvement respectively. Trans-mesothelial Resistance (R(TM)) across the pleural membrane was determined as a permeability indicator. Immunohistochemistry for IGF-1 receptors was performed.
  
  
  RESULTS
  IGF-1 increased R(TM) when added on the interstitial surface for all concentrations (p=.008, 1 nM-100 nM) and decreased it on the mesothelial surface for higher concentrations (p=.046, 100 nM). Amiloride and ouabain inhibited this effect. The IGF-1 Receptor Inhibitor also totally inhibited this effect. Immonuhistochemistry demonstrated the presence of IGF-1 receptors in the pleura.
  
  
  CONCLUSIONS
  It is concluded that IGF-1 changes the electrophysiology of the human parietal pleura by hindering the normal ion transportation and therefore the pleural fluid recycling process. This event is achieved after IGF-1 interaction with its receptor which is present in the human pleura.
authors.affiliation: Department of Physiology, Medical School, University of Thessaly, Larissa, Greece. kouritas@otenet.gr
authors.forename: Vasileios K
authors.initials: VK
authors.lastname: Kouritas
authors.forename: Maria
authors.initials: M
authors.lastname: Ioannou
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Desimonas
authors.forename: Konstantinos I
authors.initials: KI
authors.lastname: Gourgoulianis
authors.forename: Paschalis A
authors.initials: PA
authors.lastname: Molyvdas
authors.forename: Chrisi
authors.initials: C
authors.lastname: Hatzoglou
mesh_terms: D000368:Aged; D000584:Amiloride / Q000494:pharmacology; D002478:Cells, Cultured; D015536:Down-Regulation / Q000187:drug effects; D004563:Electrochemistry; D055724:Electrophysiological Phenomena / Q000187:drug effects; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D017136:Ion Transport / Q000187:drug effects; D007477:Ions / Q000378:metabolism*; D055442:Metabolome / Q000187:drug effects; D008875:Middle Aged; D010539:Permeability / Q000187:drug effects; D010994:Pleura / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D011485:Protein Binding / Q000187:drug effects / Q000502:physiology; D017526:Receptor, IGF Type 1 / Q000378:metabolism* / Q000502:physiology
chemical_list: D007477:Ions; D007334:Insulin-Like Growth Factor I; D000584:Amiloride; D017526:Receptor, IGF Type 1