title: [Establishment of confirmatory test for suspicious hepatitis B surface antigen positive samples].
abstract: OBJECTIVE
  Establish a confirmatory test based on ELISA, and use to verify the authenticity of HBsAg weak positive samples, pick and get rid of the false result, and avoid the mistake diagnosis.
  
  
  METHODS
  The particles (reagent A) coated by streptavidin and biotinylated HBsAb (reagent B) were mixed in different proportions, then neutralized with serum whose the COI of HBsAg > 20 by ELISA in order to identify the activity of HBsAb in confirmatory reagent. 30 pieces of HBsAg weak positive serum neutralized with the confirmatory reagent, the serum were considered to be positive if rate of decline of HBsAg COI > 50%. The results were compared to Roche confirmatory Kit.
  
  
  RESULTS
  Confirmatory reagent was able to neutralized with HBsAg. 24 of 30 pieces of HBsAg weak positive samples were judged to be positive, while 6 poeces were negative. The ELISA comfirm method is fully consistent with Roche confirmatory Kit.
  
  
  CONCLUSIONS
  The ELISA confirmatory test for suspicious HBsAg positive samples is a simple, accurate and low cost initial validation method, After further clinical trials, should be widely applied.
authors.affiliation: Beijing 302 hospital, Beijing 100039, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Yang
authors.initials: Y
authors.lastname: Rong
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.forename: Jing-Xia
authors.initials: JX
authors.lastname: Guo
authors.forename: Yong-Ji
authors.initials: YJ
authors.lastname: Song
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Ai-Xia
authors.initials: AX
authors.lastname: Liu
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Yang
authors.forename: Bo-An
authors.initials: BA
authors.lastname: Li
authors.forename: Yuan-Li
authors.initials: YL
authors.lastname: Mao
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006509:Hepatitis B / Q000097:blood / Q000175:diagnosis*; D006510:Hepatitis B Antibodies / Q000097:blood; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006801:Humans
chemical_list: D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens