title: Selective precipitation of large RNAs.
abstract: For reasons that are somewhat mysterious, "large" RNAs ≥100 nucleotides long can be selectively separated from small RNAs <100 nucleotides long by precipitation with either LiCl or PEG/NaCl. These methods are very straightforward, and are valuable for enriching for very small RNAs (i.e., miRNAs) in unprecipitated material while retaining all other RNAs in the precipitate.
authors.forename: Timothy W
authors.initials: TW
authors.lastname: Nilsen
mesh_terms: D005590:Fractional Precipitation / Q000379:methods*; D018021:Lithium Chloride / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification*; D012965:Sodium Chloride / Q000737:chemistry
chemical_list: D011092:Polyethylene Glycols; D012965:Sodium Chloride; D012313:RNA; D018021:Lithium Chloride