title: Performance comparison of Affymetrix SNP6.0 and cytogenetic 2.7M whole-genome microarrays in complex cancer samples.
abstract: The Affymetrix cytogenetic 2.7M whole-genome microarray (Cyto2.7M) detects genomic aberrations. The Cyto2.7M array has increased coverage in regions with cancer-related genes, ~4-fold reduced processing time, and 5-fold reduced input requirements (100 ng) compared to the commonly used Affymetrix SNP6.0 genome-wide microarray (SNP6.0). We set out to compare the performance of these microarrays on cancer samples containing complex genomic changes. We analyzed genomic DNA from 8 lymphoma samples and 1 blood sample using both SNP6.0 and Cyto2.7M microarrays. We compared the arrays with respect to 4 parameters, including detection of copy number variations (CNV), CNV boundaries, the actual copy number (CN) assigned to the aberrations, and loss of heterozygosity. The CN state of selected regions was validated by quantitative PCR. Very high consistency between arrays on all parameters tested was observed, hence only 30 of 224 aberrations disagreed on the CN state, corresponding to a total of ~12 Mb or 0.06% of the analyzed base pairs. Thus, the SNP6.0 and Cyto2.7M arrays are equally well suited to detect genomic aberrations in complex samples such as cancer samples. With reduced processing time and lower input requirements, the Cyto2.7M array enables genomic analysis of samples where only limited DNA is available.
authors.affiliation: Department of Haematology, Aalborg Hospital, Aarhus University Hospital, Aalborg, Denmark. j.boedker@rn.dk
authors.forename: J S
authors.initials: JS
authors.lastname: Bødker
authors.forename: C
authors.initials: C
authors.lastname: Gyrup
authors.forename: P
authors.initials: P
authors.lastname: Johansen
authors.forename: A
authors.initials: A
authors.lastname: Schmitz
authors.forename: J
authors.initials: J
authors.lastname: Madsen
authors.forename: H E
authors.initials: HE
authors.lastname: Johnsen
authors.forename: M
authors.initials: M
authors.lastname: Bøgsted
authors.forename: K
authors.initials: K
authors.lastname: Dybkær
authors.forename: M
authors.initials: M
authors.lastname: Nyegaard
mesh_terms: D002869:Chromosome Aberrations; D020732:Cytogenetic Analysis / Q000379:methods*; D056915:DNA Copy Number Variations; D004273:DNA, Neoplasm / Q000235:genetics; D015894:Genome, Human / Q000235:genetics*; D006801:Humans; D019656:Loss of Heterozygosity; D009369:Neoplasms / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results
chemical_list: D004273:DNA, Neoplasm