title: Protective effects of Sonchus asper against KBrO3 induced lipid peroxidation in rats.
abstract: BACKGROUND
  Sonchus asper is traditionally used in Pakistan for the treatment of reproductive dysfunction and oxidative stress. The present investigation was aimed to evaluate chloroform extract of Sonchus asper (SACE) against potassium bromate-induced reproductive stress in male rats.
  
  
  METHODS
  20 mg/kg body weight (b.w.) potassium bromate (KBrO3) was induced in 36 rats for four weeks and checked the protective efficacy of SACE at various hormonal imbalances, alteration of antioxidant enzymes, and DNA fragmentation levels. High performance chromatography (HPLC) was used for determination of bioactive constituents responsible.
  
  
  RESULTS
  The level of hormonal secretion was significantly altered by potassium bromate. DNA fragmentation%, activity of antioxidant enzymes; catalase (CAT), peroxidase (POD), superoxide dismutase (SOD) and phase II metabolizing enzymes viz; glutathione reductase (GSR), glutathione peroxidase (GSHpx), glutathione-S-tansase (GST) and reduced glutathione (GSH) was decreased while hydrogen per oxide contents and thiobarbituric acid reactive substances (TBARS) were increased with KBrO3 treatment. Treatment with SACE effectively ameliorated the alterations in the biochemical markers; hormonal and molecular levels while HPLC characterization revealed the presence of catechin, kaempferol, rutin and quercetin.
  
  
  CONCLUSIONS
  Protective effects of Sonchus asper vs. KBrO3 induced lipid peroxidation might be due to bioactive compound present in SACE.
authors.affiliation: Department of Biotechnology, Faculty of Sciences, University of Science and Technology Bannu, Bannu, Pakistan. Rahmatgul_81@yahoo.com
authors.forename: Rahmat Ali
authors.initials: RA
authors.lastname: Khan
authors.forename: Muhammad Rashid
authors.initials: MR
authors.lastname: Khan
authors.forename: Sumaira
authors.initials: S
authors.lastname: Sahreen
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001959:Bromates / Q000633:toxicity; D053938:DNA Fragmentation / Q000187:drug effects*; D004798:Enzymes / Q000097:blood / Q000378:metabolism; D006728:Hormones / Q000097:blood / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D031184:Sonchus / Q000737:chemistry; D013737:Testis / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
chemical_list: D000975:Antioxidants; D001959:Bromates; D004798:Enzymes; D006728:Hormones; D010936:Plant Extracts; D020011:Protective Agents; C019536:potassium bromate