title: Nonmedially assembled F-actin cables incorporate into the actomyosin ring in fission yeast.
abstract: In many eukaryotes, cytokinesis requires the assembly and constriction of an actomyosin-based contractile ring. Despite the central role of this ring in cytokinesis, the mechanism of F-actin assembly and accumulation in the ring is not fully understood. In this paper, we investigate the mechanism of F-actin assembly during cytokinesis in Schizosaccharomyces pombe using lifeact as a probe to monitor actin dynamics. Previous work has shown that F-actin in the actomyosin ring is assembled de novo at the division site. Surprisingly, we find that a significant fraction of F-actin in the ring was recruited from formin-Cdc12p nucleated long actin cables that were generated at multiple nonmedial locations and incorporated into the ring by a combination of myosin II and myosin V activities. Our results, together with findings in animal cells, suggest that de novo F-actin assembly at the division site and directed transport of F-actin cables assembled elsewhere can contribute to ring assembly.
authors.affiliation: Cell Division Laboratory, Temasek Life Sciences Laboratory, Singapore 117604.
authors.forename: Junqi
authors.initials: J
authors.lastname: Huang
authors.forename: Yinyi
authors.initials: Y
authors.lastname: Huang
authors.forename: Haochen
authors.initials: H
authors.lastname: Yu
authors.forename: Dhivya
authors.initials: D
authors.lastname: Subramanian
authors.forename: Anup
authors.initials: A
authors.lastname: Padmanabhan
authors.forename: Rahul
authors.initials: R
authors.lastname: Thadani
authors.forename: Yaqiong
authors.initials: Y
authors.lastname: Tao
authors.forename: Xie
authors.initials: X
authors.lastname: Tang
authors.forename: Roland
authors.initials: R
authors.lastname: Wedlich-Soldner
authors.forename: Mohan K
authors.initials: MK
authors.lastname: Balasubramanian
mesh_terms: D000199:Actins / Q000378:metabolism*; D000205:Actomyosin / Q000378:metabolism*; D048749:Cytokinesis; D012568:Schizosaccharomyces / Q000166:cytology* / Q000378:metabolism*
chemical_list: D000199:Actins; D000205:Actomyosin