title: Bringing single-molecule spectroscopy to macromolecular protein complexes.
abstract: Single-molecule fluorescence spectroscopy offers real-time, nanometer-resolution information. Over the past two decades, this emerging single-molecule technique has been rapidly adopted to investigate the structural dynamics and biological functions of proteins. Despite this remarkable achievement, single-molecule fluorescence techniques must be extended to macromolecular protein complexes that are physiologically more relevant for functional studies. In this review, we present recent major breakthroughs for investigating protein complexes within cell extracts using single-molecule fluorescence. We outline the challenges, future prospects and potential applications of these new single-molecule fluorescence techniques in biological and clinical research.
authors.affiliation: Kavli Institute of NanoScience, Department of BioNanoScience, Delft University of Technology, 2628CJ, Delft, The Netherlands. c.joo@tudelft.nl
authors.forename: Chirlmin
authors.initials: C
authors.lastname: Joo
authors.forename: Mohamed
authors.initials: M
authors.lastname: Fareh
authors.forename: V Narry
authors.initials: VN
authors.lastname: Kim
mesh_terms: D000818:Animals; D006801:Humans; D008958:Models, Molecular; D046912:Multiprotein Complexes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D046912:Multiprotein Complexes