title: Liposomes as vehicles for transferring low molecular weight compounds into periwinkle protoplasts.
abstract: The uptake of liposomal contents into Catharanthus roseus protoplasts has been quantitated. Hi.phest uptake was obtained from positively charged vesicles (1.09% of vesicle contents per 10(7) protoplasts) in the presence of 10% w/v polyethylene glycol 4000. Significant uptake was also observed from negatively charged vesicles (0.1% of vesicle contents per 10(7) protoplasts) in the presence of 25% polyethylene glycol 1540. The uptake of vesicle contents from negatively charged liposomes was confirmed by light microscopy after treatment of the protoplasts with liposomes loaded with the fluorescent dye 6-carboxyfluorescein. About 10% of the treated protoplasts exhibited intracellular fluorescence. No uptake from neutral liposomes was detected.
authors.affiliation: Plant Biotechnology Institute, National Research Council of Canada, Saskatoon, Canada S7N 0W9.
authors.forename: A J
authors.initials: AJ
authors.lastname: Cutler
authors.forename: P D
authors.initials: PD
authors.lastname: Shargool
authors.forename: W G
authors.initials: WG
authors.lastname: Kurz
authors.forename: F
authors.initials: F
authors.lastname: Constabel