title: Diterpenoids from the soft coral Sinularia maxima and their inhibitory effects on lipopolysaccharide-stimulated production of pro-inflammatory cytokines in bone marrow-derived dendritic cells.
abstract: Nine new diterpenoids, termed sinumaximols A-I (1-3, 5-9, 12), together with three known compounds (4, 10, 11), were isolated from the methanol extract of the soft coral Sinularia maxima. Their structures were elucidated on the basis of extensive spectroscopic methods, including one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS). The isolated compounds were evaluated for their inhibitory effects on the lipopolysaccharide (LPS)-stimulated production of pro-inflammatory cytokines in bone marrow-derived dendritic cells (BMDCs). Among them, compounds 2, 3, and 11 were potent inhibitors of LPS-stimulated interleukin-12 (IL-12) p40 with half-maximal inhibitory concentration (IC(50)) values ranging from 4.35±0.12 to 18.04±0.21 µM. Compounds 2, 3, and 11 showed moderate inhibitory activity on IL-6 production with IC(50) values ranging from 17.72±0.31 to 59.77±2.34 µM.
authors.affiliation: Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Nghiado, Caugiay, Hanoi, Vietnam.
authors.forename: Nguyen Phuong
authors.initials: NP
authors.lastname: Thao
authors.forename: Nguyen Hoai
authors.initials: NH
authors.lastname: Nam
authors.forename: Nguyen Xuan
authors.initials: NX
authors.lastname: Cuong
authors.forename: Tran Hong
authors.initials: TH
authors.lastname: Quang
authors.forename: Pham The
authors.initials: PT
authors.lastname: Tung
authors.forename: Bui Huu
authors.initials: BH
authors.lastname: Tai
authors.forename: Bui Thi Thuy
authors.initials: BT
authors.lastname: Luyen
authors.forename: Doobyeong
authors.initials: D
authors.lastname: Chae
authors.forename: Sohyun
authors.initials: S
authors.lastname: Kim
authors.forename: Young-Sang
authors.initials: YS
authors.lastname: Koh
authors.forename: Phan Van
authors.initials: PV
authors.lastname: Kiem
authors.forename: Chau Van
authors.initials: CV
authors.lastname: Minh
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis*; D003713:Dendritic Cells / Q000187:drug effects* / Q000378:metabolism; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008968:Molecular Conformation; D013329:Structure-Activity Relationship
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; D004224:Diterpenes; D008070:Lipopolysaccharides