title: Shiga toxigenic and atypical enteropathogenic Escherichia coli in the feces and carcasses of slaughtered pigs.
abstract: Escherichia coli is a pathogen of major importance in swine and public health. To determine the prevalence of Shiga toxigenic E. coli (STEC) and enteropathogenic E. coli (EPEC), samples were collected from the feces and carcasses of swines. In total, 441 samples were collected in four samplings, of which 141 samples tested positive for either the stx1, stx2, and/or eae genes. From the positive samples, one STEC and 15 atypical EPEC (aEPEC) isolates were obtained, and all originated from the same sampling. In addition to eae, lpfA(O157/OI-141), ehxA, toxB, and lpfA(O113) were present in the aEPEC isolates. The only stx2-containing isolate carried stx2e and belonged to serotype O103:HNT. Resistance to four or more antimicrobials was found in almost half of the isolates, and some isolates shared the same fingerprint patterns by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). The presence of certain virulence genes and the high level of resistance to antimicrobials, as well as the possible fecal contamination of carcasses showed that some of the isolates are of public health concern.
authors.affiliation: Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, Universidade Estadual Paulista, São Paulo, Brasil. lissa_bio@yahoo.com
authors.forename: Clarissa Araújo
authors.initials: CA
authors.lastname: Borges
authors.forename: Lívia Gerbasi
authors.initials: LG
authors.lastname: Beraldo
authors.forename: Renato Pariz
authors.initials: RP
authors.lastname: Maluta
authors.forename: Marita Vedovelli
authors.initials: MV
authors.lastname: Cardozo
authors.forename: Beatriz Ernestina Cabilio
authors.initials: BE
authors.lastname: Guth
authors.forename: Everlon Cid
authors.initials: EC
authors.lastname: Rigobelo
authors.forename: Fernando Antônio
authors.initials: FA
authors.lastname: de Ávila
mesh_terms: D000003:Abattoirs; D000818:Animals; D024901:Drug Resistance, Multiple, Bacterial; D054308:Enteropathogenic Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005243:Feces / Q000382:microbiology; D005516:Food Microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012703:Serotyping; D022603:Shiga Toxins / Q000235:genetics; D054323:Shiga-Toxigenic Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology*; D037521:Virulence Factors / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; D022603:Shiga Toxins; D037521:Virulence Factors