title: Identification of proteins differentially expressed in adriamycin-resistant (pumc-91/ADM) and parental (pumc-91) human bladder cancer cell lines by proteome analysis.
abstract: OBJECTIVE
  Resistance to chemotherapy drugs remains a difficult problem in bladder cancer treatment. Protein expression is an important factor underlying multidrug resistance (MDR) in bladder cancer. The aim of the study was to explore differentially expressed proteins responsible for MDR between an adriamycin-resistant human bladder cancer cell line (pumc-91/ADM) and its parental cell line (pumc-91).
  
  
  METHODS
  Two-dimensional gel electrophoresis (2-DE) combining image analysis was used to screen the differentially expressed protein spots between the pumc-91/ADM and pumc-91 cell lines. Then, the protein spots were identified using MALDI-TOF/TOF mass spectrometry. Among the identified proteins, annexin A2 (ANXA2) and nucleophosmin (NPM1) were then further verified using RT-PCR and Western blot analysis.
  
  
  RESULTS
  A total of 30 proteins, including 19 up-regulated and 11 down-regulated proteins, were successfully identified in pumc-91/ADM. According to their different functions, these 30 proteins were classified into 12 categories. Annexin A2 (ANXA2) and nucleophosmin (NPM1) were up-regulated in pumc-91/ADM compared with pumc-91.
  
  
  CONCLUSIONS
  The proteins identified may have an important clinical significance in MDR, and ANXA2 and NPM1 may take part in mechanism of MDR in bladder cancer.
authors.affiliation: Department of Clinical Laboratory Center, Shijitan Hospital Affiliated with Capital Medical University, Beijing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Meng
authors.forename: Ting
authors.initials: T
authors.lastname: Lei
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Jin
authors.initials: J
authors.lastname: Zhao
authors.forename: Xu-Hong
authors.initials: XH
authors.lastname: Zhao
authors.forename: Man
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000903:Antibiotics, Antineoplastic / Q000627:therapeutic use; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000627:therapeutic use*; D019008:Drug Resistance, Neoplasm; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000032:analysis* / Q000378:metabolism; D000090243:Nucleophosmin; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000473:pathology; D054928:Validation Studies as Topic
chemical_list: D000903:Antibiotics, Antineoplastic; C000716967:NPM1 protein, human; D009363:Neoplasm Proteins; D020543:Proteome; D000090243:Nucleophosmin; D004317:Doxorubicin