title: A fluorescent probe for rapid detection of thiols and imaging of thiols reducing repair and H2O2 oxidative stress cycles in living cells.
abstract: A diselenide containing fluorescent probe based on a fluorescein scaffold for thiols was developed. The fluorescent probe exhibited rapid response, high selectivity and reversibility. Confocal fluorescence microscopy was used to visualize the redox changes mediated by thiols and reactive oxygen species in living HeLa cells.
authors.affiliation: State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics (DICP), Chinese Academy of Sciences (CAS), 457 Zhongshan Road, Dalian 116023, PR China.
authors.forename: Zhangrong
authors.initials: Z
authors.lastname: Lou
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Sun
authors.forename: Songqiu
authors.initials: S
authors.lastname: Yang
authors.forename: Bingshuai
authors.initials: B
authors.lastname: Wang
authors.forename: Keli
authors.initials: K
authors.lastname: Han
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry*; D018613:Microscopy, Confocal; D015394:Molecular Structure; D018384:Oxidative Stress; D012643:Selenium / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000032:analysis*; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds; D006861:Hydrogen Peroxide; D012643:Selenium