title: Expression, purification, crystallization and preliminary X-ray analysis of NAD(P)H-dependent carbonyl reductase specifically expressed in thyroidectomized chicken fatty liver.
abstract: An NAD(P)H-dependent carbonyl reductase specifically expressed in thyroidectomized chicken fatty liver was crystallized using the sitting-drop vapour-diffusion method with polyethylene glycol 300 as the precipitant. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a=104.26, b=81.32, c=77.27 Å, β=119.43°, and diffracted to 1.86 Å resolution on beamline NE3A at the Photon Factory. The overall Rmerge was 5.4% and the data completeness was 99.4%.
authors.affiliation: Department of Bioscience, School of Agriculture, Tokai University, Aso, Kumamoto 869-1404, Japan. kyoneda@agri.u-tokai.ac.jp
authors.forename: Kazunari
authors.initials: K
authors.lastname: Yoneda
authors.forename: Yudai
authors.initials: Y
authors.lastname: Fukuda
authors.forename: Takeshi
authors.initials: T
authors.lastname: Shibata
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Araki
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nikki
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Sakuraba
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Ohshima
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D002645:Chickens / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D005234:Fatty Liver / Q000201:enzymology* / Q000378:metabolism; D009249:NADP / Q000737:chemistry / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism; D013965:Thyroidectomy
chemical_list: D011092:Polyethylene Glycols; D009249:NADP; D000429:Alcohol Oxidoreductases