title: Cyclooxygenase-2 expression in skeletal muscle of knockout mice suffering Duchenne muscular dystrophy.
abstract: The purpose of the present study was to investigate the role of cyclooxygenase-2 (COX-2) expression in fibrotic lesion in mdx mice. A total of six male C57BL/10 mice and six C57BL/10-DMD/mdx were distributed into two groups: control and animals with Duchenne muscular dystrophy (DMD). The medial part of gastrocnemius muscle was evaluated being the specimens stained with hematoxylin and eosin (H&E) and Sirius Red under normal and polarized light to differentiate type I (red and yellow) and III (green) collagen. COX-2 expression was assessed by immunohistochemistry. The results revealed histopathological changes in C57BL/10-DMD/mdx as depicted by regenerating fibers. Sirius Red stain showed a substantial increase in the amount of type I collagen of mdx mice. DMD induced a strong COX-2 immunoexpression in intercellular space. Taken together, our results are consistent with the notion that necrotic and fibrotic lesions are able to increase COX-2 expression in DMD.
authors.affiliation: Department of Biosciences, Federal University of São Paulo, UNIFESP, Av Ana Costa 95, Vila Mathias, Santos, SP 11060-001, Brazil.
authors.forename: Flavia
authors.initials: F
authors.lastname: de Oliveira
authors.forename: De Oliveira
authors.initials: de O
authors.lastname: Flavia
authors.forename: Hananiah Tardivo
authors.initials: HT
authors.lastname: Quintana
authors.forename: Jeferson André
authors.initials: JA
authors.lastname: Bortolin
authors.forename: Odair Alfredo
authors.initials: OA
authors.lastname: Gomes
authors.forename: Edson Aparecido
authors.initials: EA
authors.lastname: Liberti
authors.forename: Daniel Araki
authors.initials: DA
authors.lastname: Ribeiro
mesh_terms: D000818:Animals; D051546:Cyclooxygenase 2 / Q000096:biosynthesis* / Q000378:metabolism; D016189:Dystrophin / Q000172:deficiency* / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D020388:Muscular Dystrophy, Duchenne / Q000201:enzymology* / Q000473:pathology
chemical_list: D016189:Dystrophin; D051546:Cyclooxygenase 2