title: Identification of a gene responsible for amido black decolorization isolated from Amycolatopsis orientalis.
abstract: Investigation into the biodegradative capability of several actinomycetes led to the discovery of many strains possessing an ability to decolorize a variety of azo and triphenylmethane dyes. Of particular interest is an Amycolatopsis sp. isolate which displayed the ability to decolorize the azo dye amido black. Through the screening of a DNA library a 4.5 kbp fragment coding for the potential decolorization gene was identified. The sequencing of this gene fragment led to the prediction of seven open reading frames encoding a polyprenyl synthetase, cupin-2 conserved barrel domain, transcriptional regulator, membrane protein, DNA-damage inducible gene and two hypothetical proteins. A gene coding for a protein consisting of 312 amino acids with 77 % similarity to a conserved hypothetical protein in Amycolatopsis mediterranei was tentatively identified as the enzyme of interest. This is the first report of an amido black biodegrading gene identified in this species.
authors.affiliation: Department of Genetics, University of Witwatersrand, Braamfontein, P.O. Box 1038, Johannesburg, South Africa. mchengalroyen@gmail.com
authors.forename: M D
authors.initials: MD
authors.lastname: Chengalroyen
authors.forename: E R
authors.initials: ER
authors.lastname: Dabbs
mesh_terms: D000192:Actinomycetales / Q000201:enzymology* / Q000235:genetics*; D000580:Amido Black / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001711:Biotransformation; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D015723:Gene Library; D005798:Genes, Bacterial; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D000580:Amido Black