title: Novel oncogene-induced metastatic prostate cancer cell lines define human prostate cancer progression signatures.
abstract: Herein, murine prostate cancer cell lines, generated via selective transduction with a single oncogene (c-Myc, Ha-Ras, and v-Src), showed oncogene-specific prostate cancer molecular signatures that were recapitulated in human prostate cancer and developed lung metastasis in immune-competent mice. Interrogation of two independent retrospective cohorts of patient samples using the oncogene signature showed an ability to distinguish tumor from normal prostate with a predictive value for prostate cancer of 98% to 99%. In a blinded study, the signature algorithm showed independent substratification of reduced recurrence-free survival by Kaplan-Meier analysis. The generation of new oncogene-specific prostate cancer cell lines that recapitulate human prostate cancer gene expression, which metastasize in immune-competent mice, are a valuable new resource for testing targeted therapy, whereas the molecular signatures identified herein provides further value over current gene signature markers of prediction and outcome.
authors.affiliation: Department of Cancer Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Ju
authors.forename: Adam
authors.initials: A
authors.lastname: Ertel
authors.forename: Mathew C
authors.initials: MC
authors.lastname: Casimiro
authors.forename: Zuoren
authors.initials: Z
authors.lastname: Yu
authors.forename: Hui
authors.initials: H
authors.lastname: Meng
authors.forename: Peter A
authors.initials: PA
authors.lastname: McCue
authors.forename: Rhonda
authors.initials: R
authors.lastname: Walters
authors.forename: Paolo
authors.initials: P
authors.lastname: Fortina
authors.forename: Michael P
authors.initials: MP
authors.lastname: Lisanti
authors.forename: Richard G
authors.initials: RG
authors.lastname: Pestell
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D018450:Disease Progression; D018572:Disease-Free Survival; D016259:Genes, myc; D011905:Genes, ras; D016391:Genes, src; D008175:Lung Neoplasms / Q000556:secondary; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009362:Neoplasm Metastasis; D009857:Oncogenes; D011237:Predictive Value of Tests; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D059467:Transcriptome