title: Nitrated fatty acids: synthesis and measurement.
abstract: Nitrated fatty acids are the product of nitrogen dioxide reaction with unsaturated fatty acids. The discovery of peroxynitrite and peroxidase-induced nitration of biomolecules led to the initial reports of endogenous nitrated fatty acids. These species increase during ischemia/reperfusion, but concentrations are often at or near the limits of detection. Here, we describe multiple methods for nitrated fatty acid synthesis and sample extraction from complex biological matrices and a rigorous method of qualitative and quantitative detection of nitrated fatty acids by liquid chromatography-mass spectrometry. In addition, optimized instrument conditions and caveats regarding data interpretation are discussed.
authors.affiliation: Department of Pharmacology & Chemical Biology, University of Pittsburgh, Pittsburgh, PA 15261, USA.
authors.forename: Steven R
authors.initials: SR
authors.lastname: Woodcock
authors.forename: Gustavo
authors.initials: G
authors.lastname: Bonacci
authors.forename: Stacy L
authors.initials: SL
authors.lastname: Gelhaus
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Schopfer
mesh_terms: D005227:Fatty Acids / Q000032:analysis* / Q000138:chemical synthesis*; D009566:Nitrates / Q000032:analysis* / Q000138:chemical synthesis*
chemical_list: D005227:Fatty Acids; D009566:Nitrates