title: A 286 bp upstream regulatory region of a rice anther-specific gene, OSIPP3, confers pollen-specific expression in Arabidopsis.
abstract: OSIPP3 gene (coding for pectin methylesterase inhibitor protein) was isolated from a pre-pollinated inflorescence-specific cDNA library by differential screening of stage-specific libraries from Oryza sativa. OSIPP3 is present in the genome of rice as a single copy gene. OSIPP3 gene was expressed exclusively in the pre-pollinated spikelets of rice. Upstream regulatory region (URR) of OSIPP3 was isolated and a series of 5'-deletions were cloned upstream of GUS reporter gene and were used to transform Arabidopsis. OSIPP3_del1 and del2 transgenic plants showed GUS expression in root, anther and silique, while OSIPP3_del3 showed GUS activity only in anthers and siliques. Pollen-specific expression was observed in case of plants harboring OSIPP3_del4 construct. It can, therefore, be concluded that the OSIPP3 URR between -178 and +108 bp is necessary for conferring pollen-specific expression in Arabidopsis.
authors.affiliation: Department of Plant Molecular Biology, Interdisciplinary Centre for Plant Genomics, University of Delhi South Campus, New Delhi, 110021, India.
authors.forename: Reema
authors.initials: R
authors.lastname: Khurana
authors.forename: Hitesh
authors.initials: H
authors.lastname: Kathuria
authors.forename: Arnab
authors.initials: A
authors.lastname: Mukhopadhyay
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kapoor
authors.forename: Akhilesh K
authors.initials: AK
authors.lastname: Tyagi
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D019677:Artificial Gene Fusion; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D005966:Glucuronidase / Q000032:analysis / Q000235:genetics; D012275:Oryza / Q000235:genetics*; D011058:Pollen / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D017384:Sequence Deletion
chemical_list: D005966:Glucuronidase