title: Novel wound dressing based on nanofibrous PHBV-keratin mats.
abstract: Keratin is an important protein used for wound healing and tissue recovery. In this study, keratin was first extracted from raw materials and chemically modified to obtain stable keratin (m-keratin). The raw and m-keratin were examined by Raman spectroscopy. The molecular weight of the m-keratin was analysed by SDS-PAGE. The m-keratin was then blended with poly(hydroxybutylate-co-hydroxyvalerate) (PHBV) and electrospun to afford nanofibrous mats. These mats were characterized by field emission scanning electron microscopy (FE-SEM), electron spectroscopy for chemical analysis (ESCA) and atomic force microscopy (AFM). From the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) data, it was found that introduction of keratin enhanced cell proliferation. From wound-healing test and histological examination results, it was shown that the composite mats accelerated wound recovery remarkably as compared to the PHBV control. It was concluded that PHBV-keratin may be a good candidate as a wound dressing.
authors.affiliation: Department of Polymer Science, Kyungpook National University, Daegu, Korea.|Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, People's Republic of China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Department of Polymer Science, Kyungpook National University, Daegu, Korea.
authors.forename: Jia
authors.initials: J
authors.lastname: Geng
authors.affiliation: Department of Polymer Science, Kyungpook National University, Daegu, Korea.
authors.forename: Zhicai
authors.initials: Z
authors.lastname: Xing
authors.affiliation: Department of Immunology, School of Medicine, Kyungpook National University, Daegu, Korea.
authors.forename: Kyoung-Jin
authors.initials: KJ
authors.lastname: Shim
authors.affiliation: Department of Immunology, School of Medicine, Kyungpook National University, Daegu, Korea.
authors.forename: Insook
authors.initials: I
authors.lastname: Han
authors.affiliation: Department of Immunology, School of Medicine, Kyungpook National University, Daegu, Korea.
authors.forename: Jung-Chul
authors.initials: JC
authors.lastname: Kim
authors.affiliation: Department of Polymer Science, Kyungpook National University, Daegu, Korea.
authors.forename: Inn-Kyu
authors.initials: IK
authors.lastname: Kang
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Shen
mesh_terms: D000818:Animals; D001458:Bandages; D004591:Electrophoresis, Polyacrylamide Gel; D007633:Keratins / Q000737:chemistry; D051379:Mice; D008819:Mice, Nude; D018625:Microscopy, Atomic Force; D041681:NIH 3T3 Cells; D057139:Nanofibers; D011091:Polyesters; D013059:Spectrum Analysis, Raman; D013778:Tetrazolium Salts; D013844:Thiazoles; D014945:Wound Healing; D000094122:Polyhydroxybutyrates
chemical_list: D007633:Keratins; D011091:Polyesters; D013778:Tetrazolium Salts; D013844:Thiazoles; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C022616:thiazolyl blue