title: Identification of genes involved in indole-3-butyric acid-induced adventitious root formation in nodal cuttings of Camellia sinensis (L.) by suppression subtractive hybridization.
abstract: The plant hormone auxin plays a key role in adventitious rooting. To increase our understanding of genes involved in adventitious root formation, we identified transcripts differentially expressed in single nodal cuttings of Camellia sinensis treated with or without indole-3-butyric acid (IBA) by suppressive subtractive hybridization (SSH). A total of 77 differentially expressed transcripts, including 70 up-regulated and 7 down-regulated sequences, were identified in tea cuttings under IBA treatment. Seven candidate transcripts were selected and analyzed for their response to IBA, and IAA by real time RT-PCR. All these transcripts were up regulated by at least two folds one day after IBA treatment. Meanwhile, IAA showed less positive effects on the expression of candidate transcripts. The full-length cDNA of a F-box/kelch gene was also isolated and found to be similar to a group of At1g23390 like genes. These unigenes provided a new source for mining genes related to adventitious root formation, which facilitate our understanding of relative fundamental metabolism.
authors.affiliation: National Center for Tea Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences (TRICAAS), 9 Meiling South Road, Hangzhou, Zhejiang 310008, PR China.
authors.forename: Kang
authors.initials: K
authors.lastname: Wei
authors.forename: Liyuan
authors.initials: L
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Cheng
authors.forename: Chengcai
authors.initials: C
authors.lastname: Zhang
authors.forename: Chunlei
authors.initials: C
authors.lastname: Ma
authors.forename: Liqun
authors.initials: L
authors.lastname: Zhang
authors.forename: Wuyun
authors.initials: W
authors.lastname: Gong
authors.forename: Liyun
authors.initials: L
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D028241:Camellia sinensis / Q000235:genetics* / Q000254:growth & development; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant / Q000235:genetics; D007210:Indoleacetic Acids / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics; D018517:Plant Roots / Q000235:genetics* / Q000254:growth & development; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D007210:Indoleacetic Acids; D007211:Indoles; D010937:Plant Growth Regulators; D010940:Plant Proteins; C014612:indolebutyric acid