title: Thiol reactive probes and chemosensors.
abstract: Thiols are important molecules in the environment and in biological processes. Cysteine (Cys), homocysteine (Hcy), glutathione (GSH) and hydrogen sulfide (H(2)S) play critical roles in a variety of physiological and pathological processes. The selective detection of thiols using reaction-based probes and sensors is very important in basic research and in disease diagnosis. This review focuses on the design of fluorescent and colorimetric probes and sensors for thiol detection. Thiol detection methods include probes and labeling agents based on nucleophilic addition and substitution, Michael addition, disulfide bond or Se-N bond cleavage, metal-sulfur interactions and more. Probes for H(2)S are based on nucleophilic cyclization, reduction and metal sulfide formation. Thiol probe and chemosensor design strategies and mechanism of action are discussed in this review.
authors.affiliation: Department of Chemistry, Center for Biotechnology and Drug Design, Georgia State University, Atlanta, GA 30302, USA. hpeng2@gsu.edu
authors.forename: Hanjing
authors.initials: H
authors.lastname: Peng
authors.forename: Weixuan
authors.initials: W
authors.lastname: Chen
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Cheng
authors.forename: Lovemore
authors.initials: L
authors.lastname: Hakuna
authors.forename: Robert
authors.initials: R
authors.lastname: Strongin
authors.forename: Binghe
authors.initials: B
authors.lastname: Wang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D057230:Limit of Detection; D051379:Mice; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds