title: Choleretic activity of Gentiana lutea ssp. symphyandra in rats.
abstract: Effects of an ethanolic extract prepared from G. lutea ssp. symphyandra roots on the bile production and liver in rats were investigated. Bile flows of rats which were treated by a single i.p. dose of CCl(4) 24 h prior to experiments were measured after the cannulation of bile duct under urethane anaesthesia. After an equilibration period of 1 h, the lyophilized extract were administered intraduodenally (500 mg/kg i.p.), while control animals received physiological saline only. To monitor the effect of multiple dose therapy, rats received the same dose of G. lutea ssp. symphyandra extract for 3 days (2 days prior to CCl(4) administration) and their bile flows were measured after the cannulation. In all groups, bile samples were collected for 3 h with 15 min intervals. After the completion of bile flow experiment, rat livers were removed and put in neutral formaldehyde solution (10%) for the histological examination. According to results obtained, multiple dose treatment of rats with the plant extract normalized the decreased bile flow due CCl(4), whereas single dose therapy was ineffective on the impaired bile flow. These data indicate that the extract prepared from Gentiana lutea ssp. symphyandra roots has a hepatoprotective activity.
authors.affiliation: Medicinal and Aromatic Plant and Drug Research Centre (TBAM), Faculty of Pharmacy, Anadolu University, Eskişehir, Turkey; Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskişehir, Turkey.
authors.forename: N
authors.initials: N
authors.lastname: Oztürk
authors.forename: T
authors.initials: T
authors.lastname: Herekman-Demir
authors.forename: Y
authors.initials: Y
authors.lastname: Oztürk
authors.forename: B
authors.initials: B
authors.lastname: Bozan
authors.forename: K H
authors.initials: KH
authors.lastname: Başer