title: Prostratin exhibits both replication enhancing and inhibiting effects on FIV infection of feline CD4+ T-cells.
abstract: The phorbol ester Prostratin may either stimulate or inhibit human immunodeficiency virus-1 (HIV-1) replication. Here we report that Prostratin also exhibits a similar dual action upon feline immunodeficiency virus (FIV) replication in an IL-2-dependent feline CD4(+) T-cell line (MYA-1). While withdrawal of IL-2 halted FIV spread, Prostratin rescued virus production and cell viability, mimicking the functions of the cytokine. Conversely, FIV grew rapidly in the presence of IL-2 and this was inhibited by Prostratin. In contrast to HIV-1, Prostratin mediated inhibition of FIV through means other than blocking virus entry. Co-application of the protein kinase C (PKC) inhibitor Gö6850 with Prostratin reversed both the inhibitory and stimulatory effects, suggesting that PKC is crucial for FIV replication.
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Glasgow, United Kingdom. c.chan.1@research.gla.ac.uk
authors.forename: Chi Ngai
authors.initials: CN
authors.lastname: Chan
authors.forename: Elizabeth L
authors.initials: EL
authors.lastname: McMonagle
authors.forename: Margaret J
authors.initials: MJ
authors.lastname: Hosie
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Willett
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000821:virology*; D002415:Cats; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015497:HIV-1 / Q000187:drug effects / Q000502:physiology; D006801:Humans; D016089:Immunodeficiency Virus, Feline / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology*; D007376:Interleukin-2 / Q000494:pharmacology; D008212:Lymphocyte Depletion; D010703:Phorbol Esters / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism; D015398:Signal Transduction; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D007376:Interleukin-2; D010703:Phorbol Esters; C070999:prostratin; D011493:Protein Kinase C