title: Complementary methods for contact hypersensitivity (CHS) evaluation in mice.
abstract: Contact hypersensitivity (CHS) is an experimental model of allergic contact dermatitis (ACD) that can be studied in mice. For CHS responses, mice are immunized by painting with a reactive hapten, such as 1-fluoro-4,6-dinitrobenzene (DNFB), on the shaved abdominal and chest skin. Subsequently, the ears are challenged with diluted hapten, eliciting 'hypersensitive' ear-swelling reactions, which can be measured with a micrometer. In this manuscript we present complementary methods that can be used to evaluate CHS in mice that include: ear weight, vascular permeability, myeloperoxidase (MPO) activity, IFN-γ concentration in ear extracts and also IFN-γ production by auricular lymph node cells (ELNC). The biochemical evaluation of CHS can be also supported by proliferation assay, measurement of IFN-γ production by skin-draining lymph node cells employing ELISA test and by evaluation of IFN-γ(+) TCRαβ CD8 cells with the use of flow cytometry.
authors.affiliation: Department of Medical Biology, Jagiellonian University College of Medicine, Kraków, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Zemelka-Wiącek
authors.forename: Monika
authors.initials: M
authors.lastname: Majewska-Szczepanik
authors.forename: Wiesław
authors.initials: W
authors.lastname: Pyrczak
authors.forename: Marian
authors.initials: M
authors.lastname: Szczepanik
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002199:Capillary Permeability / Q000276:immunology; D049109:Cell Proliferation; D003877:Dermatitis, Contact / Q000175:diagnosis* / Q000276:immunology*; D004139:Dinitrofluorobenzene / Q000276:immunology; D004431:Ear, External / Q000098:blood supply / Q000276:immunology* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006241:Haptens / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009929:Organ Size / Q000276:immunology; D009195:Peroxidase / Q000276:immunology / Q000378:metabolism; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology / Q000378:metabolism; D012680:Sensitivity and Specificity; D012867:Skin / Q000098:blood supply / Q000276:immunology* / Q000473:pathology; D013047:Specific Pathogen-Free Organisms
chemical_list: D006241:Haptens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D007371:Interferon-gamma; D004139:Dinitrofluorobenzene; D009195:Peroxidase