title: In vitro antibacterial, cytotoxicity and haemolytic activities and phytochemical analysis of seagrasses from the Gulf of Mannar, South India.
abstract: It is essential to study the phytochemical constituents and toxicological properties of seagrasses when considering their food applications. Aqueous methanolic extracts of six seagrasses were evaluated for their antibacterial, cytotoxic (brine shrimp leathality assay) and haemolytic activity. Thin layer chromatography (TLC) and phytochemical analysis were used to compare the phytochemical profiles of six seagrasses. Among the six seagrasses examined, Halodule pinifolia and Cymodocea rotundata showed predominant growth inhibitory activity against all the tested human pathogens. Cytotoxicity of seagrass extracts against nauplii of Artemia salina revealed that Syringodium isoetifolium exhibited lesser toxicity with LC(50) value of 699.096 μg/ml. Of all the seagrasses tested, H. pinifolia recorded the minimum haemolytic activity of 2.07±0.63% at 1000 μg/ml concentration. Phytochemical analysis showed the presence of common plant chemical constituents which varied with respect to species. The present findings suggest the possible pharmacological applications of selected seagrasses that can be used as food ingredients.
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
authors.forename: Rengasamy
authors.initials: R
authors.lastname: Ragupathi Raja Kannan
authors.forename: Radjassegarin
authors.initials: R
authors.lastname: Arumugam
authors.forename: Palanisamy
authors.initials: P
authors.lastname: Iyapparaj
authors.forename: Thirunavukarasu
authors.initials: T
authors.lastname: Thangaradjou
authors.forename: Perumal
authors.initials: P
authors.lastname: Anantharaman
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001156:Artemia / Q000187:drug effects; D001419:Bacteria / Q000187:drug effects; D001681:Biological Assay; D001773:Blood Cells / Q000187:drug effects; D002855:Chromatography, Thin Layer; D003603:Cytotoxins / Q000737:chemistry / Q000494:pharmacology*; D053878:Hemolytic Agents / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D007194:India; D007928:Lethal Dose 50; D019684:Magnoliopsida / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D003603:Cytotoxins; D053878:Hemolytic Agents; D010936:Plant Extracts