title: Identification of carbapenem-resistant Acinetobacter baumannii clones using infrared spectroscopy.
abstract: In this work we assessed the discriminatory ability of Fourier-transform Infrared Spectroscopy (FTIR) in 22 representative isolates from a collection of 318 carbapenem-hydrolyzing class D β -lactamases (CHDL)-producing Acinetobacter spp. (5 hospitals; 2001-2008) previously characterized by DNA-based typing methods. FTIR spectra were acquired with a Bruker spectrometer and analyzed with support of several chemometric tools. The results showed that FTIR spectroscopy was able to distinguish the main CHDL-producing Acinetobacter baumannii lineages causing infection in Portugal, the ST103 carrying blaOXA-58 , ST98 carrying blaOXA-24/40 and ST92 carrying blaOXA-23 . Moreover, this study revealed distinctive phenotypic features of A. baumannii lineages causing infections that might justify different epidemic potential. Spectroscopy may arise as a low cost and easily to perform alternative for typing A. baumannii isolates.
authors.affiliation: REQUIMTE, Laboratório de Microbiologia, Departamento de Ciências Biológicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
authors.forename: Clara
authors.initials: C
authors.lastname: Sousa
authors.forename: Filipa
authors.initials: F
authors.lastname: Grosso
authors.forename: Luís
authors.initials: L
authors.lastname: Meirinhos-Soares
authors.forename: Luísa
authors.initials: L
authors.lastname: Peixe
authors.forename: João
authors.initials: J
authors.lastname: Lopes
mesh_terms: D040981:Acinetobacter baumannii / Q000166:cytology / Q000187:drug effects* / Q000302:isolation & purification*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015780:Carbapenems / Q000494:pharmacology*; D002999:Clone Cells / Q000187:drug effects; D016002:Discriminant Analysis; D024881:Drug Resistance, Bacterial; D006801:Humans; D016018:Least-Squares Analysis; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems