title: Crystal structure of decaprenylphosphoryl-β- D-ribose 2'-epimerase from Mycobacterium smegmatis.
abstract: Decaprenylphosphoryl-β-D-ribose 2'-epimerase (DprE1) is an essential enzyme in the biosynthesis of cell wall components and a target for development of anti-tuberculosis drugs. We determined the crystal structure of a truncated form of DprE1 from Mycobacterium smegmatis in two crystal forms to up to 2.35 Å resolution. The structure extends from residue 75 to the C-terminus and shares homology with FAD-dependent oxidoreductases of the vanillyl-alcohol oxidase family including the DprE1 homologue from M. tuberculosis. The M. smegmatis DprE1 structure reported here provides further insights into the active site geometry of this tuberculosis drug target.
authors.affiliation: Department of Molecular Biology, Cellular Biology and Biochemistry, Brown University, Providence, Rhode Island 02912, USA.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Gerwald
authors.initials: G
authors.lastname: Jogl
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004791:Enzyme Inhibitors / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry; D005798:Genes, Bacterial; D016018:Least-Squares Analysis; D020102:Mycobacterium smegmatis / Q000201:enzymology* / Q000235:genetics; D010088:Oxidoreductases / Q000737:chemistry; D010627:Phenethylamines / Q000737:chemistry; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D019998:Racemases and Epimerases / Q000037:antagonists & inhibitors / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; C092859:CT 435; D004791:Enzyme Inhibitors; D010627:Phenethylamines; D011994:Recombinant Proteins; D005182:Flavin-Adenine Dinucleotide; D010088:Oxidoreductases; D019998:Racemases and Epimerases