title: Partial sequence analysis of B2L gene of Brazilian orf viruses from sheep and goats.
abstract: We herein describe the partial nucleotide sequencing and phylogenetic analysis of the B2L gene of seventeen Brazilian orf viruses (ORFV). Seventeen viruses were recovered from outbreaks of contagious ecthyma in sheep and goats in four states in Southern and Northeast country, and three from commercial vaccines. Most analyzed viruses were associated with outbreaks of classical contagious ecthyma, with lip, nostrils and labial commissure involvement, yet udder/teat, feet, vulvar and disseminated lesions were also reported in some cases. Nucleotide sequence analysis revealed a high degree of B2L similarity among sheep sequences (>99%) regardless the geographic origin, and a remarkable high identity for the two goat isolates (>99.8%), with similarity dropping to below 99% when comparing viruses from the two species. A phylogenetic tree grouped most sheep and goat viruses on different branches. In addition, sequence alignment allowed the identification of up to six scattered nucleotide changes that were predominant and more consistent in goat isolates, including a number of sequences from other continents. Thus, in spite of the high nucleotide similarity, different degrees of similarity and discrete nucleotide changes in the B2L gene may help in grouping ORFV viruses according to host species.
authors.affiliation: Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Av. Roraima, 1000 Santa Maria, RS 97105-900, Brazil.
authors.forename: Candice
authors.initials: C
authors.lastname: Schmidt
authors.forename: Juliana F
authors.initials: JF
authors.lastname: Cargnelutti
authors.forename: Mário C S
authors.initials: MC
authors.lastname: Brum
authors.forename: Carolina K
authors.initials: CK
authors.lastname: Traesel
authors.forename: Rudi
authors.initials: R
authors.lastname: Weiblen
authors.forename: Eduardo F
authors.initials: EF
authors.lastname: Flores
mesh_terms: D000818:Animals; D001483:Base Sequence; D001938:Brazil / Q000453:epidemiology; D004196:Disease Outbreaks / Q000662:veterinary; D004474:Ecthyma, Contagious / Q000453:epidemiology / Q000821:virology*; D015511:Goat Diseases / Q000453:epidemiology / Q000821:virology*; D006041:Goats; D008969:Molecular Sequence Data; D009923:Orf virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D034561:Sheep, Domestic