title: Up-regulated miR-145 expression inhibits porcine preadipocytes differentiation by targeting IRS1.
abstract: Generally, most miRNAs that were up-regulated during differentiation promoted adipogenesis, but our research indicated that up-regulation of miR-145 in porcine preadipocytes did not promote but inhibit adipogenesis. In this study, miR-145 was significantly up-regulated during porcine dedifferentiated fat (DFAT) cells differentiation. In miR-145 overexpressed DFAT cells, adipogenesis was inhibited and triglycerides accumulation was decreased after hormone stimulation (P<0.05). Furthermore, up-regulation of miR-145 expression repressed induction of mRNA levels of adipogenic markers, such as CCAAT/enhancer-binding protein α (C/EBPα), and peroxisome proliferator-activated receptor γ2 (PPARγ2). These effects caused by miR-145 overexpression were mediated by Insulin receptor substrate 1 (IRS1) as a mechanism. These data suggested that induced miR-145 expression during differentiation could inhibit adipogenesis by targeting IRS1, and miR-145 may be novel agent for adipose tissue engineering.
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong, P. R. China.
authors.forename: Yunxue
authors.initials: Y
authors.lastname: Guo
authors.forename: Yaosheng
authors.initials: Y
authors.lastname: Chen
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Luxi
authors.initials: L
authors.lastname: Chen
authors.forename: Delin
authors.initials: D
authors.lastname: Mo
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D055504:Insulin Receptor Substrate Proteins / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism / Q000502:physiology*; D013552:Swine; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; D055504:Insulin Receptor Substrate Proteins; D035683:MicroRNAs