title: Novel configuration of a myosin II transient intermediate analogue revealed by quick-freeze deep-etch replica electron microscopy.
abstract: In the present paper, we described our attempt to characterize the rough three-dimensional features of the structural analogue of the key intermediate of myosin's cross-bridge cycle. Using quick-freeze deep-etch replica electron microscopy, we observed that actin-attached myosin during in vitro sliding was bent superficially as postulated by the conventional hypothesis, but in the opposite direction of the putative pre-power-stroke configuration, as for ADP·Vi (inorganic vanadate)-bound myosin. We searched for the conformational species with a similar appearance and found that SH1-SH2 (thiols 1 and 2)-cross-linked myosin is a good candidate. To characterize such small asymmetric structures, we employed a new pattern-recognition procedure that accommodates the metal-replicated samples. In this method, the best-matched views of the target microscopic images were selected from a comprehensive set of images simulated from known atomic co-ordinates of relevant proteins. Together with effective morphological filtering, we could define the conformational species and the view angles of the catalytic domain and the lever arm cropped from averaged images of disulfide-cross-linked myosin. Whereas the catalytic domain of the new conformer closely resembled the pPDM (N,N'-p-phenylenedimaleimide)-treated, but SH2 Lys705-cross-linked, structure (PDB code 1L2O), a minor product of the same cross-linking reaction, the lever arm projected differently. Using separately determined view angles of the catalytic domain and the lever arm, we built a model of disulfide-cross-linked myosin. Further combination with the 'displacement-mapping' procedure enabled us to reconstruct the global three-dimensional envelope of the unusual structure whose lever arm orientation is compatible with our reports on the actin-sliding cross-bridge structure. Assuming this conformer as the structural analogue of the transient intermediate during actin sliding, the power stroke of the lever arm might accompany the reversal of the disorganized SH1 helix.
authors.affiliation: Division of Biomolecular Imaging, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Kimori
authors.forename: Norio
authors.initials: N
authors.lastname: Baba
authors.forename: Eisaku
authors.initials: E
authors.lastname: Katayama
mesh_terms: D000818:Animals; D002645:Chickens; D003432:Cross-Linking Reagents / Q000737:chemistry; D005613:Freeze Etching; D008301:Maleimides / Q000737:chemistry; D008854:Microscopy, Electron; D015879:Myosin Subfragments / Q000737:chemistry / Q000648:ultrastructure; D024462:Myosin Type II / Q000737:chemistry* / Q000648:ultrastructure; D011487:Protein Conformation; D013438:Sulfhydryl Compounds / Q000737:chemistry; D014638:Vanadates / Q000737:chemistry
chemical_list: D003432:Cross-Linking Reagents; D008301:Maleimides; D015879:Myosin Subfragments; D013438:Sulfhydryl Compounds; D014638:Vanadates; C011328:N,N'-4-phenylenedimaleimide; D024462:Myosin Type II