title: Amperometric glucose biosensor based on electroconductive hydrogels.
abstract: Fabrication of an enzyme amperometric biosensor for glucose via electropolymerization of pyrrole in the presence of glucose oxidase onto a hydrogel coated platinum electrode is hereby established as a viable biotransducer fabrication method. Platinum micro- (φ=25 μm) and macro- (φ=100 μm) electrodes were electrochemically activated and chemically modified with 3-aminopropyl-trimethoxysilane (APTMS), functionalized with acryloyl(polyethyleneglycol)-N-hydroxysuccinamide (ACRL-PEG-NHS), dipped into a polyHEMA based hydrogel cocktail and UV cross-linked. Electropolymerization of Py in the presence of GOx produced glucose responsive biotransducers that showed; (i) a 4-fold reduction in sensitivity compared with directly electropolymerized PPy films, (ii) an electropolymerization charge density dependence of biotransducer sensitivity and enzyme activity that was maximal at 1.0 mC/cm(2) with an apparent K(M) of 33 mM, (iii) interference screening of ascorbic acid and (iv) a temporal increase in sensitivity with storage over a 17 days period. This method has the ability to precisely and quantitatively add enzyme catalytic bioactivity to metal or semiconductor biointerfaces for applications in biosensors, bioelectronics and bionics.
authors.affiliation: Center for Bioelectronics, Biosensors and Biochips (C3B), Clemson University Advanced Materials Center, 100 Technology Drive, Anderson, South Carolina, SC 29625, USA.
authors.forename: Christian N
authors.initials: CN
authors.lastname: Kotanen
authors.forename: Chaker
authors.initials: C
authors.lastname: Tlili
authors.forename: Anthony
authors.initials: A
authors.lastname: Guiseppi-Elie
mesh_terms: D001205:Ascorbic Acid / Q000737:chemistry; D015374:Biosensing Techniques; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism; D020100:Hydrogels / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D010984:Platinum / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*
chemical_list: D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D020100:Hydrogels; D011108:Polymers; D010984:Platinum; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose; D001205:Ascorbic Acid