title: Retinoic acid improves recovery after nephrectomy and decreases renal TGF-β1 expression. Gender-related effects.
abstract: End-stage renal disease is a cause for death worldwide. Renal transplant is a therapeutic alternative, restricted by the scant number of donors. Function of the donor kidney is under risk of adverse circumstances such as fibrosis, where profibrotic effect of transforming growth factor beta 1 (TGF-β1) plays a key role. Efforts to diminish risks of damage in the remnant kidney of the donor are required. Vitamin A represents one alternative. It has beneficial effects on some nephropathies, mainly those related to oxidative stress. It also participates in normal intrauterine renal development. We studied the effect of all-trans retinoic acid (ATRA), active form of vitamin A, on postnephrectomy compensatory growth, in male or female rats. Compensatory growth and renal function were evaluated on four experimental groups: Control without treatment (CTL), ATRA-treated intact rats (CTL + RA), nephrectomized rats (NFX), and ATRA-treated nephrectomized rats (NFX + RA). We evaluated glomerular function (inulin clearance), tubular function (fractional excretions of sodium and potassium), and urinary flow. Renal mass was also estimated. In ATRA-treated animals, compensatory growth was higher than in nephrectomized rats without treatment. Hyperfiltration after nephrectomy was less intense in ATRA-treated female than in male rats. In tubular functions, effect of ATRA was more evident in female than in male rats. Glomerular expression of TGF-β1 was lower in ATRA-treated animals than in controls. ATRA reduced intensity and duration of compensatory changes after nephrectomy, improving recovery.
authors.affiliation: Pharmacology Department, Center for Research and Advanced Studies, National Polytechnic Institute of Mexico (Cinvestav-IPN), C.P. 07360, Mexico City, Mexico.
authors.forename: Dealmy
authors.initials: D
authors.lastname: Delgadillo
authors.forename: Olivier
authors.initials: O
authors.lastname: Barbier
authors.forename: Gerardo
authors.initials: G
authors.lastname: Sierra
authors.forename: Jose L
authors.initials: JL
authors.lastname: Reyes
mesh_terms: D000818:Animals; D005260:Female; D005355:Fibrosis; D005455:Fluorescent Antibody Technique; D005919:Glomerular Filtration Rate / Q000187:drug effects; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009392:Nephrectomy; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012727:Sex Characteristics; D053773:Transforming Growth Factor beta1 / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D014212:Tretinoin / Q000008:administration & dosage / Q000627:therapeutic use*; D014554:Urination / Q000187:drug effects
chemical_list: D053773:Transforming Growth Factor beta1; D014212:Tretinoin