title: HER2 overexpression-mediated inflammatory signaling enhances mammosphere formation through up-regulation of aryl hydrocarbon receptor transcription.
abstract: The interaction between HER2 and aryl hydrocarbon receptor (AhR) signaling cascades during mammosphere formation of MCF-7 cells was studied. A newly established clonal MCF-7 cell line (HER2-5), stably overexpressing HER2, showed significantly enhanced levels of AhR mRNA and protein compared with MCF-7 cells. AhR was required for the HER2-mediated induction of interleukin-6 mRNA and for mammosphere formation in HER2-5 and MCF-7 cells. Mammosphere forming efficiency was suppressed by an AhR antagonist in a dose-dependent manner, as well as by knockdown of AhR. Taken together, these results indicate that AhR enhances mammosphere formation by human HER2-overexpressing breast cancer cells.
authors.affiliation: Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zhao
authors.forename: Shiori
authors.initials: S
authors.lastname: Ohara
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Kanno
authors.forename: Yudai
authors.initials: Y
authors.lastname: Midorikawa
authors.forename: Momoka
authors.initials: M
authors.lastname: Nakayama
authors.forename: Minami
authors.initials: M
authors.lastname: Makimura
authors.forename: Youngjin
authors.initials: Y
authors.lastname: Park
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Inouye
mesh_terms: D001943:Breast Neoplasms / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D018929:Cell Culture Techniques; D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D018336:Receptors, Aryl Hydrocarbon / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D018874:Spheroids, Cellular; D014158:Transcription, Genetic; D014162:Transfection; D015854:Up-Regulation
chemical_list: D018719:Erb-b2 Receptor Tyrosine Kinases; D018336:Receptors, Aryl Hydrocarbon