title: Proteomic profiling of expression of proteasomal subunits from livers of mice treated with diethylnitrosamine.
abstract: The liver plays a central role in transforming and clearing chemicals and is susceptible to the toxicity from these agents. Diethylnitrosamine is metabolized primarily in the liver by cytochrome P-450 and can cause DNA damage. The 26S proteasome is a large proteolytic complex that degrades ubiquitinated proteins, and regulates many physiological processes. We used proteomics-based approaches to examine expressional differences of liver proteasomal subunits from diethylnitrosamine-treated mice. The expression of most proteasomal subunits was observed to be upregulated in the analysis of 2DE and MALDI-TOF MS/MS. Some of these differentially expressed proteasomal subunits were further confirmed by Western blot, RT-PCR, and immunohistochemistry. Our results provided useful information on the relationship between the proteasomal complex and related diseases.
authors.affiliation: School of Life Sciences, Xiamen University, Xiamen, Fujian, P R China.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Yuan
authors.forename: Jia
authors.initials: J
authors.lastname: Lu
authors.forename: Pan
authors.initials: P
authors.lastname: You
authors.forename: Zengming
authors.initials: Z
authors.lastname: Yang
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Yang
authors.forename: Qiling
authors.initials: Q
authors.lastname: Ma
authors.forename: Tao
authors.initials: T
authors.lastname: Tao
mesh_terms: D000818:Animals; D004052:Diethylnitrosamine; D015180:Electrophoresis, Gel, Two-Dimensional; D006651:Histocytochemistry; D006965:Hyperplasia; D008099:Liver / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology* / Q000473:pathology; D008297:Male; D051379:Mice; D016133:Polymerase Chain Reaction; D046988:Proteasome Endopeptidase Complex / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D021122:Protein Subunits; D020543:Proteome / Q000737:chemistry / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods
chemical_list: D021122:Protein Subunits; D020543:Proteome; D004052:Diethylnitrosamine; D046988:Proteasome Endopeptidase Complex