title: The anti-obesity effect of natural vanadium-containing Jeju ground water.
abstract: This study investigated the anti-obesity effects of Jeju ground water containing the vanadium components S1 (8.0 ± 0.9 μg/l) and S3 (26.0 ± 2.09 μg/l) on the differentiation of 3 T3-L1 preadipocytes and obesity in mice that were fed a high-fat diet (HFD). The 3 T3-L1 preadipocyte cells were cultured and differentiated in media consisting of Jeju ground water (S1, S3) or deionized water (DW) containing dexamethasone, isobutylmethylxanthine, and insulin. Oil Red O staining showed that lipid accumulation was attenuated in adipocyte cells treated with Jeju ground water. S3 significantly decreased peroxisome-activated receptor γ and CCAAT-enhancer-binding protein α mRNA expression levels, which play major roles in the transcriptional control of adipogenesis, compared to DW. Furthermore, mRNA expression levels of targeted genes, such as adipocyte fatty acid, lipoprotein lipase, and leptin, were decreased by S3 treatment compared with the control group. In mice with HFD-induced obesity, Jeju ground water decreased HFD-induced body weight gain and reduced total cholesterol, triglyceride, and glucose levels in the plasma compared to control mice. Taken together, Jeju ground water inhibits preadipocyte differentiation and adipogenesis in obesity animal models.
authors.affiliation: DNA Damage Response Network Center, Bio Engineering BD, Chosun University School of Medicine, 375 Seosuk-dong, Gwangju, South Korea.
authors.forename: Seon-Joo
authors.initials: SJ
authors.lastname: Park
authors.forename: Cha-Kyung
authors.initials: CK
authors.lastname: Youn
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun
authors.forename: Ho Jin
authors.initials: HJ
authors.lastname: You
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D001391:Azo Compounds / Q000737:chemistry; D015153:Blotting, Western; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000737:chemistry / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D003907:Dexamethasone / Q000737:chemistry; D059305:Diet, High-Fat / Q000009:adverse effects; D005347:Fibroblasts / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D060587:Groundwater / Q000737:chemistry*; D007328:Insulin / Q000737:chemistry; D050356:Lipid Metabolism; D008071:Lipoprotein Lipase / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000628:therapy*; D047495:PPAR gamma / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D056910:Republic of Korea; D014158:Transcription, Genetic; D014639:Vanadium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D019440:Anti-Obesity Agents; D001391:Azo Compounds; D022763:CCAAT-Enhancer-Binding Protein-alpha; D003470:Culture Media; D007328:Insulin; D047495:PPAR gamma; D012333:RNA, Messenger; D014639:Vanadium; D003907:Dexamethasone; D008071:Lipoprotein Lipase; C011049:oil red O