title: Allelic gene expression imbalance of bovine IGF2, LEP and CCL2 genes in liver, kidney and pituitary.
abstract: Allelic expression imbalance (AEI) is an important genetic factor being the cause of differences in phenotypic traits that can be heritable. Studying AEI can be useful in searching for factors that modulate gene expression and help to understand molecular mechanisms underlying phenotypic changes. Although it was commonly recognized in many species and we know many genes show allelic expression imbalance, this phenomena was not studied on a larger scale in cattle. Using the pyrosequencing method we analyzed a set of 29 bovine genes in order to find those that have preferential allelic expression. The study was conducted in three tissues: liver, pituitary and kindey. Out of the studied group of genes 3 of them-LEP (leptin), IGF2 (insulin-like growth factor 2), CCL2 (chemokine C-C motif ligand 2) showed allelic expression imbalance.
authors.affiliation: Department of Molecular Biology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences (IGAB PAS), Jastrzębiec, 05-552, Magdalenka, Poland. olbromski.rafal@gmail.com
authors.forename: R
authors.initials: R
authors.lastname: Olbromski
authors.forename: E
authors.initials: E
authors.lastname: Siadkowska
authors.forename: B
authors.initials: B
authors.lastname: Zelazowska
authors.forename: L
authors.initials: L
authors.lastname: Zwierzchowski
mesh_terms: D000483:Alleles; D000818:Animals; D002417:Cattle / Q000235:genetics* / Q000378:metabolism; D018932:Chemokine CCL2 / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005786:Gene Expression Regulation; D007335:Insulin-Like Growth Factor II / Q000235:genetics* / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D020738:Leptin / Q000235:genetics* / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D010902:Pituitary Gland / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
chemical_list: D018932:Chemokine CCL2; D020738:Leptin; D007335:Insulin-Like Growth Factor II