title: Implantation study of small-caliber "biotube" vascular grafts in a rat model.
abstract: We developed autologous vascular grafts, called "biotubes," by simple and safe in-body tissue architecture technology, which is a practical concept of regenerative medicine, without using special sterile conditions or complicated in vitro cell treatment processes. In this study, biotubes of extremely small caliber were first auto-implanted to rat abdominal aortas. Biotubes were prepared by placing silicone rods (outer diameter 1.5 mm, length 30 mm) used as a mold into dorsal subcutaneous pouches in rats for 4 weeks. After argatroban coating, the obtained biotubes were auto-implanted to abdominal aortas (n = 6) by end-to-end anastomosis using a custom-designed sutureless vascular connecting system under microscopic guidance. Graft status was evaluated by contrast-free time-of-flight magnetic resonance angiography (TOF-MRA). All grafts were harvested at 12 weeks after implantation. The patency rate was 66.7 % (4/6). MRA showed little stenosis and no aneurysmal dilation in all biotubes. The original biotube had wall thickness of about 56.2 ± 26.5 μm at the middle portion and mainly random and sparse collagen fibers and fibroblasts. After implantation, the wall thickness was 235.8 ± 24.8 μm. In addition, native-like vascular structure was regenerated, which included (1) a completely endothelialized luminal surface, (2) a mesh-like elastin fiber network, and (3) regular circumferential orientation of collagen fibers and α-SMA positive cells. Biotubes could be used as small-caliber vascular prostheses that greatly facilitate the healing process and exhibit excellent biocompatibility in vascular regenerative medicine.
authors.affiliation: Division of Medical Engineering and Materials, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka, 565-8565, Japan. yamanami@koto.kpu-m.ac.jp
authors.forename: Masashi
authors.initials: M
authors.lastname: Yamanami
authors.forename: Hatsue
authors.initials: H
authors.lastname: Ishibashi-Ueda
authors.forename: Akihide
authors.initials: A
authors.lastname: Yamamoto
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Iida
authors.forename: Taiji
authors.initials: T
authors.lastname: Watanabe
authors.forename: Keiichi
authors.initials: K
authors.lastname: Kanda
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Yaku
authors.forename: Yasuhide
authors.initials: Y
authors.lastname: Nakayama
mesh_terms: D000818:Animals; D001807:Blood Vessel Prosthesis; D005260:Female; D008422:Materials Testing; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering; D058017:Vascular Grafting / Q000379:methods*