title: Genotyping of Trichomonas vaginalis isolates in Iran by using single stranded conformational polymorphism-PCR technique and internal transcribed spacer regions.
abstract: Infection with Trichomonas vaginalis, the causative agent of human urogenital   infection, is the most prevalent nonviral sexually transmitted disease worldwide. In spite of   the high prevalence and medical importance of trichomoniasis, there is little knowledge   about genetic epidemiology and genetic characterisation of this parasite. For this purpose, a   Single Stranded Conformation Polymorphism-PCR (SSCP-PCR) typing method was conducted   for Iranian T. vaginalis isolates using 5.8s ribosomal gene (rRNA gene) and the flanking   internal transcribed spacer (ITS) regions. Nine hundred and fifty vaginal swab samples were   examined in which 50 (5.3%) samples were parasitologically positive and used for molecular   identification based on SSCP-PCR and nucleotide sequence analyses. Results of the SSCP   analysis showed two distinct reproducible banding patterns (I, II) which were confirmed by   nucleotide sequence analysis in the ITS1 regions. Frequencies of the SSCP banding patterns   I and II were 84% (42/50) and 16% (8/50), respectively. In conclusion, SSCP-PCR analysis   provided a reliable and sensitive method for strain genotyping of T. vaginalis based on the   ITS1/5.8s/ITS2 region. This finding may help us gain more information about correlation   between genetic properties and biological features of this parasite.
authors.affiliation: Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Matini
authors.forename: M
authors.initials: M
authors.lastname: Rezaeian
authors.forename: M
authors.initials: M
authors.lastname: Mohebali
authors.forename: A H
authors.initials: AH
authors.lastname: Maghsood
authors.forename: S
authors.initials: S
authors.lastname: Rabiee
authors.forename: A
authors.initials: A
authors.lastname: Rahimi-Foroushani
authors.forename: M
authors.initials: M
authors.lastname: Fallah
authors.forename: A
authors.initials: A
authors.lastname: Miahipour
authors.forename: S
authors.initials: S
authors.lastname: Rezaie
mesh_terms: D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D007492:Iran; D017720:Molecular Epidemiology / Q000379:methods; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014246:Trichomonas vaginalis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D017931:DNA Primers; D016054:DNA, Protozoan; D021903:DNA, Ribosomal Spacer; D012340:RNA, Ribosomal, 5.8S