title: Genome sequence of Corynebacterium pseudotuberculosis biovar equi strain 258 and prediction of antigenic targets to improve biotechnological vaccine production.
abstract: Corynebacterium pseudotuberculosis is the causative agent of several veterinary diseases in a broad range of economically important hosts, which can vary from caseous lymphadenitis in sheep and goats (biovar ovis) to ulcerative lymphangitis in cattle and horses (biovar equi). Existing vaccines against C. pseudotuberculosis are mainly intended for small ruminants and, even in these hosts, they still present remarkable limitations. In this study, we present the complete genome sequence of C. pseudotuberculosis biovar equi strain 258, isolated from a horse with ulcerative lymphangitis. The genome has a total size of 2,314,404 bp and contains 2088 predicted protein-coding regions. Using in silico analysis, eleven pathogenicity islands were detected in the genome sequence of C. pseudotuberculosis 258. The application of a reverse vaccinology strategy identified 49 putative antigenic proteins, which can be used as candidate vaccine targets in future works.
authors.affiliation: CLIB Graduate Cluster Industrial Biotechnology, Centrum für Biotechnologie, Universität Bielefeld, 33615 Bielefeld, Germany. siomars@gmail.com
authors.forename: Siomar C
authors.initials: SC
authors.lastname: Soares
authors.forename: Eva
authors.initials: E
authors.lastname: Trost
authors.forename: Rommel T J
authors.initials: RT
authors.lastname: Ramos
authors.forename: Adriana R
authors.initials: AR
authors.lastname: Carneiro
authors.forename: Anderson R
authors.initials: AR
authors.lastname: Santos
authors.forename: Anne C
authors.initials: AC
authors.lastname: Pinto
authors.forename: Eudes
authors.initials: E
authors.lastname: Barbosa
authors.forename: Flávia
authors.initials: F
authors.lastname: Aburjaile
authors.forename: Amjad
authors.initials: A
authors.lastname: Ali
authors.forename: Carlos A A
authors.initials: CA
authors.lastname: Diniz
authors.forename: Syed S
authors.initials: SS
authors.lastname: Hassan
authors.forename: Karina
authors.initials: K
authors.lastname: Fiaux
authors.forename: Luis C
authors.initials: LC
authors.lastname: Guimarães
authors.forename: Syeda M
authors.initials: SM
authors.lastname: Bakhtiar
authors.forename: Ulisses
authors.initials: U
authors.lastname: Pereira
authors.forename: Sintia S
authors.initials: SS
authors.lastname: Almeida
authors.forename: Vinícius A C
authors.initials: VA
authors.lastname: Abreu
authors.forename: Flávia S
authors.initials: FS
authors.lastname: Rocha
authors.forename: Fernanda A
authors.initials: FA
authors.lastname: Dorella
authors.forename: Anderson
authors.initials: A
authors.lastname: Miyoshi
authors.forename: Artur
authors.initials: A
authors.lastname: Silva
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo
authors.forename: Andreas
authors.initials: A
authors.lastname: Tauch
mesh_terms: D000820:Animal Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D001428:Bacterial Vaccines / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D001709:Biotechnology / Q000379:methods*; D003354:Corynebacterium Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary; D016925:Corynebacterium pseudotuberculosis / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D016680:Genome, Bacterial; D044404:Genomic Islands; D006736:Horses
chemical_list: D000942:Antigens, Bacterial; D001428:Bacterial Vaccines