title: Enhanced bone healing of rat tooth sockets after administration of epidermal growth factor (EGF) carried by liposome.
abstract: Considering the potential use of growth factors carried by liposomes for bone repair, this study aimed to assess the progress of bone healing process in injured alveoli of rats after administering EGF within liposomes. For this assessment we used 48 male Wistar rats that had their maxillary second molar extracted and separated into 5 groups: sockets filled with blood clot (BC), treated with empty liposome (L), PBS (P), EGF in PBS (EGF-P) and EGF in liposome (EGF-L). The animals were sacrificed after 3, 7, 14 and 21 days after surgery. Histological, histomorphometric and immunohistochemistry analysis were performed to evaluate new bone and blood vessels formation as well as the expression of fibronectin and collagen type III, two determinant proteins for early wound regeneration. Our analysis showed a continuous transformation of sockets during all stages of wound healing. Nevertheless, groups BC, L, P and EGF-P followed a regular time for regeneration significantly different from the EGF-L group, which showed faster recovering. A higher expression of fibronectin and type III collagen in the group EGF-L after 3 and 7 days of surgery was observed and might be explained by the ability of the liposome to deliver EGF in a controlled manner, stimulating mesenchymal cells migration and osteoblast differentiation. As liposome efficiently regulated the availability of EGF without risks for its function and protected the factor from early absorption and degradation, the present work indicates that liposomes can be successful used as carriers for controlled delivery of growth factors in bone healing.
authors.affiliation: Laboratory of Biopathology and Molecular Biology, University of Uberaba, MG, Brazil.
authors.forename: Luciana
authors.initials: L
authors.lastname: Marquez
authors.forename: Fernando Antônio Mauad
authors.initials: FA
authors.lastname: de Abreu
authors.forename: Cynthia Lopes
authors.initials: CL
authors.lastname: Ferreira
authors.forename: Guilherme Dias
authors.initials: GD
authors.lastname: Alves
authors.forename: Melissa Nunes
authors.initials: MN
authors.lastname: Miziara
authors.forename: José Bento
authors.initials: JB
authors.lastname: Alves
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D003692:Delayed-Action Preparations; D004815:Epidermal Growth Factor / Q000378:metabolism* / Q000494:pharmacology; D008081:Liposomes / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D008437:Maxilla / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020390:Tooth Socket / Q000378:metabolism*; D014945:Wound Healing
chemical_list: D003692:Delayed-Action Preparations; D008081:Liposomes; D004815:Epidermal Growth Factor