title: Clonal diversity and high prevalence of OXA-58 among Acinetobacter baumannii isolates from blood cultures in a tertiary care centre in Turkey.
abstract: Genotypic diversity, antimicrobial susceptibilty, and presence of OXA-genes were assessed in 100 nosocomial Acinetobacter strains from a tertiary-care hospital, Turkey. Ninety-eight isolates were identified by AFLP library identification to Acinetobacter baumannii. Furthermore, the isolates were divided into 30 AFLP clusters and single strains at a similarity cut-off level of 90%, the defined strain level. Most of these clusters grouped together in larger clusters at a lower similarity level, indicating diversification beyond the strain level. At a similarity level of 80%, the A. baumannii isolates were allocated to eight clusters of multiple isolates (A, C, D, E, G, H, J, L) and 3 single isolates (B, F, I). Comparison of the isolates to those of the Leiden AFLP database revealed that the large cluster H (41 isolates) corresponded to a tentative novel international clone previously identified both by AFLP and MLST (CC15). Clusters D and E grouped with European (EU) clone II isolates, and cluster J with those EU clone I. Clusters A, C, G, and L could not be identified to any international clone. MLST of selected isolates of the major clusters corroborated the clone allocation by AFLP, except for the tested cluster A isolate which was identified to CC2 (EU clone II). Carbapenem resistance of 75 A. baumannii isolates was associated with the blaOXA-58-like gene or blaOXA-51-like with ISAba1 upstream. Altogether, 99% of the Acinetobacter isolates were multidrug resistant (MDR) and 77% extensively drug resistant (XDR). The findings show that multiple strains and clones MDR and XDR A. baumannii were endemic in the hospital.
authors.affiliation: Department of Infectious Diseases and Clinical Microbiology, Erciyes University Faculty of Medicine, Kayseri, Turkey. gokhanmetan@gmail.com
authors.forename: Gokhan
authors.initials: G
authors.lastname: Metan
authors.forename: Fatma
authors.initials: F
authors.lastname: Sariguzel
authors.forename: Bulent
authors.initials: B
authors.lastname: Sumerkan
authors.forename: Tanny van der
authors.initials: Tv
authors.lastname: Reijden
authors.forename: Lenie
authors.initials: L
authors.lastname: Dijkshoorn
mesh_terms: D000151:Acinetobacter Infections / Q000453:epidemiology*; D040981:Acinetobacter baumannii / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D054458:Amplified Fragment Length Polymorphism Analysis; D016000:Cluster Analysis; D003428:Cross Infection; D006801:Humans; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D015995:Prevalence; D062606:Tertiary Care Centers; D014421:Turkey; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001618:beta-Lactamases; C534889:beta-lactamase OXA-58, Acinetobacter baumannii