title: HbzF catalyzes direct hydrolysis of maleylpyruvate in the gentisate pathway of Pseudomonas alcaligenes NCIMB 9867.
abstract: HbzF from Pseudomonas alcaligenes NCIMB 9867 was purified to homogeneity as a His-tagged protein and likely a dimer by SDS-PAGE and gel filtration. This protein was demonstrated to be a novel maleylpyruvate hydrolase, catalyzing direct hydrolysis of maleylpyruvate to maleate and pyruvate, and belongs to the fumarylacetoacetate hydrolase superfamily. This study reveals the genetic determinate for the direct maleylpyruvate hydrolysis in the gentisate pathway, complementary to the well-studied maleylpyruvate isomerization route.
authors.affiliation: Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Liu
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Liu
authors.forename: Ning-Yi
authors.initials: NY
authors.lastname: Zhou
mesh_terms: D002850:Chromatography, Gel; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D006867:Hydrolases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D006868:Hydrolysis; D008298:Maleates / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010867:Pimelic Acids / Q000378:metabolism*; D055503:Protein Multimerization; D044202:Pseudomonas alcaligenes / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D019289:Pyruvic Acid / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D008298:Maleates; D010867:Pimelic Acids; C113957:maleylpyruvic acid; D019289:Pyruvic Acid; C030272:maleic acid; D006867:Hydrolases