title: Functional mapping of the lectin activity site on the β-prism domain of vibrio cholerae cytolysin: implications for the membrane pore-formation mechanism of the toxin.
abstract: Vibrio cholerae cytolysin (VCC) is a prominent member in the family of β-barrel pore-forming toxins. It induces lysis of target eukaryotic cells by forming transmembrane oligomeric β-barrel channels. VCC also exhibits prominent lectin-like activity in interacting with β1-galactosyl-terminated glycoconjugates. Apart from the cytolysin domain, VCC harbors two lectin-like domains: the β-Trefoil and the β-Prism domains; however, precise contribution of these domains in the lectin property of VCC is not known. Also, role(s) of these lectin-like domains in the mode of action of VCC remain obscure. In the present study, we show that the β-Prism domain of VCC acts as the structural scaffold to determine the lectin activity of the protein toward β1-galactosyl-terminated glycoconjugates. Toward exploring the physiological implication of the β-Prism domain, we demonstrate that the presence of the β-Prism domain-mediated lectin activity is crucial for an efficient interaction of the toxin toward the target cells. Our results also suggest that such lectin activity may act to regulate the oligomerization ability of the membrane-bound VCC toxin. Based on the data presented here, and also consistent with the existing structural information, we propose a novel mechanism of regulation imposed by the β-Prism domain's lectin activity, implicated in the process of membrane pore formation by VCC.
authors.affiliation: Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Mohali, Sector 81, SAS Nagar, Manauli, 140306, Punjab, India.
authors.forename: Anand Kumar
authors.initials: AK
authors.lastname: Rai
authors.forename: Karan
authors.initials: K
authors.lastname: Paul
authors.forename: Kausik
authors.initials: K
authors.lastname: Chattopadhyay
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002151:Calorimetry; D002942:Circular Dichroism; D003603:Cytotoxins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004912:Erythrocytes / Q000737:chemistry / Q000382:microbiology; D004926:Escherichia coli; D006001:Glycoconjugates / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D037102:Lectins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D009154:Mutation; D054353:Perforin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013329:Structure-Activity Relationship; D014734:Vibrio cholerae / Q000737:chemistry* / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D003603:Cytotoxins; D006001:Glycoconjugates; D037102:Lectins; D011994:Recombinant Proteins; D054353:Perforin