title: Decreased expression of microRNA-375 in nonsmall cell lung cancer and its clinical significance.
abstract: OBJECTIVE
  Emerging evidence has shown the association of aberrant microRNA-375 (miR-375) expression with tumourigenesis in many types of human malignancy. This prospective study characterized the contribution of miR-375 to the initiation and progression of nonsmall cell lung cancer  (NSCLC).
  
  
  METHODS
  The real-time reverse transcription-polymerase chain reaction was used to examine miR-375 levels prospectively in 96 pairs of samples of NSCLC tissue and adjacent noncancerous tissue (> 2 cm from cancer tissue). The relationship between miR-375 levels and clinico  pathological features was also explored.
  
  
  RESULTS
  MiR-375 was downregulated in 89% (85/96) of NSCLC samples compared with matched noncancerous tissue samples. Decreased miR-375 correlated significantly with advanced disease stage and lymphatic metastasis. Univariate and multivariate Cox proportional  hazard regression analyses revealed that underexpression of miR-375 was an unfavourable prognostic factor for overall survival in NSCLC.
  
  
  CONCLUSIONS
  This study suggested that miR-375 is a novel prognostic indicator in NSCLC and might be a potential target for diagnosis and gene therapy.
authors.affiliation: Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: Q
authors.initials: Q
authors.lastname: Jiang
authors.forename: N
authors.initials: N
authors.lastname: Xia
authors.forename: H
authors.initials: H
authors.lastname: Yang
authors.forename: C
authors.initials: C
authors.lastname: Hu
mesh_terms: D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000401:mortality / Q000556:secondary; D015536:Down-Regulation; D005260:Female; D015870:Gene Expression; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D015999:Multivariate Analysis; D011379:Prognosis; D016016:Proportional Hazards Models
chemical_list: C540933:MIRN375 microRNA, human; D035683:MicroRNAs