title: microRNA-214-mediated UBC9 expression in glioma.
abstract: It has been reported that ubiquitin-conjugating enzyme 9 (Ubc9), the unique enzyme2 in the sumoylation pathway, is up-regulated in many cancers. However, the expression and regulation of UBC9 in glioma remains unknown. In this study, we found that Ubc9 was up-regulated in glioma tissues and cell lines compared to a normal control. UBC9 knockdown by small interfering RNA (siRNA) affected cell proliferation and apoptosis in T98G cells. Further experiments revealed that microRNA (miR)-214 directly targeted the 3' untranslated region (UTR) of UBC9 and that there was an inverse relationship between the expression levels of miR-214 and UBC9 protein in glioma tissues and cells. miR-214 overexpression suppressed the endogenous UBC9 protein and affected T98G cell proliferation. These findings suggest that miR-214 reduction facilitates UBC9 expression and is involved in the regulation of glioma cell proliferation.
authors.affiliation: The National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, PR China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Zhao
authors.forename: Xiaochao
authors.initials: X
authors.lastname: Tan
authors.forename: Ani
authors.initials: A
authors.lastname: Zhao
authors.forename: Liyuan
authors.initials: L
authors.lastname: Zhu
authors.forename: Bin
authors.initials: B
authors.lastname: Yin
authors.forename: Jiangang
authors.initials: J
authors.lastname: Yuan
authors.forename: Boqin
authors.initials: B
authors.lastname: Qiang
authors.forename: Xiaozhong
authors.initials: X
authors.lastname: Peng
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D017209:Apoptosis; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D016022:Case-Control Studies; D049109:Cell Proliferation; D017931:DNA Primers / Q000737:chemistry; D005910:Glioma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D060787:Neoplasm Grading; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D044763:Ubiquitin-Conjugating Enzymes / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000098843:Ubiquitin-Conjugating Enzyme UBC9
chemical_list: D020413:3' Untranslated Regions; D017931:DNA Primers; C547397:MIRN214 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D044763:Ubiquitin-Conjugating Enzymes; D000098843:Ubiquitin-Conjugating Enzyme UBC9