title: The molecular profiles of neural stem cell niche in the adult subventricular zone.
abstract: Neural stem cells (NSCs) reside in a unique microenvironment called the neurogenic niche and generate functional new neurons. The neurogenic niche contains several distinct types of cells and interacts with the NSCs in the subventricular zone (SVZ) of the lateral ventricle. While several molecules produced by the niche cells have been identified to regulate adult neurogenesis, a systematic profiling of autocrine/paracrine signaling molecules in the neurogenic regions involved in maintenance, self-renewal, proliferation, and differentiation of NSCs has not been done. We took advantage of the genetic inducible fate mapping system (GIFM) and transgenic mice to isolate the SVZ niche cells including NSCs, transit-amplifying progenitors (TAPs), astrocytes, ependymal cells, and vascular endothelial cells. From the isolated cells and microdissected choroid plexus, we obtained the secretory molecule expression profiling (SMEP) of each cell type using the Signal Sequence Trap method. We identified a total of 151 genes encoding secretory or membrane proteins. In addition, we obtained the potential SMEP of NSCs using cDNA microarray technology. Through the combination of multiple screening approaches, we identified a number of candidate genes with a potential relevance for regulating the NSC behaviors, which provide new insight into the nature of neurogenic niche signals.
authors.affiliation: Program in Genomics of Differentiation, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: Cheol
authors.initials: C
authors.lastname: Lee
authors.forename: Jingqiong
authors.initials: J
authors.lastname: Hu
authors.forename: Sherry
authors.initials: S
authors.lastname: Ralls
authors.forename: Toshio
authors.initials: T
authors.lastname: Kitamura
authors.forename: Y Peng
authors.initials: YP
authors.lastname: Loh
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Yang
authors.forename: Yoh-suke
authors.initials: YS
authors.lastname: Mukouyama
authors.forename: Sohyun
authors.initials: S
authors.lastname: Ahn
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D007150:Immunohistochemistry; D020547:Lateral Ventricles / Q000378:metabolism*; D008297:Male; D051379:Mice; D058953:Neural Stem Cells / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D055153:Stem Cell Niche / Q000235:genetics / Q000502:physiology*