title: Probing nucleic acid interactions and pre-mRNA splicing by Förster Resonance Energy Transfer (FRET) microscopy.
abstract: Förster resonance energy transfer (FRET) microscopy is a powerful technique routinely used to monitor interactions between biomolecules. Here, we focus on the techniques that are used for investigating the structure and interactions of nucleic acids (NAs). We present a brief overview of the most commonly used FRET microscopy techniques, their advantages and drawbacks. We list experimental approaches recently used for either in vitro or in vivo studies. Next, we summarize how FRET contributed to the understanding of pre-mRNA splicing and spliceosome assembly.
authors.affiliation: Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083,  142 20 Prague, Czech Republic. david.stanek@img.cas.cz.
authors.forename: Eva
authors.initials: E
authors.lastname: Simková
authors.forename: David
authors.initials: D
authors.lastname: Staněk
mesh_terms: D017398:Alternative Splicing; D031541:Fluorescence Resonance Energy Transfer; D008856:Microscopy, Fluorescence; D008968:Molecular Conformation; D009696:Nucleic Acids / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D009698:Nucleoproteins / Q000378:metabolism; D011485:Protein Binding; D012322:RNA Precursors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D012326:RNA Splicing; D017381:Spliceosomes / Q000235:genetics / Q000378:metabolism
chemical_list: D009696:Nucleic Acids; D009698:Nucleoproteins; D012322:RNA Precursors