title: Fas ligand plays an important role for the production of pro-inflammatory cytokines in intervertebral disc nucleus pulposus cells.
abstract: It is suggested that pro-inflammatory cytokines, which are produced by interaction of the intervertebral nucleus pulposus cells and macrophages, may be linked to the cause of pain of the intervertebral disc herniation. This study carries out the in vitro experiments to examine the mechanism, with the use of the co-culture of an immortalized cell line of nucleus pulposus of the human intervertebral disc and the macrophage cell line. As a result, it is found that the production of pro-inflammatory cytokines is significantly larger at the co-culture group than at the independent culture group. Also, at the co-culture group of macrophages and intervertebral nucleus pulposus cells with over-expression of fas ligand (FasL), the production of pro-inflammatory cytokines is found to be far larger. Furthermore, it is found that these pro-inflammatory cytokines are produced mainly by the intervertebral nucleus pulposus cells with over-expression of FasL, and that the expression of a disintegrin and metalloproteinase (ADAM) 10, which controls the expression of FasL and activates reverse signaling inside cells, also increases. From these findings, it is suggested that FasL and ADAM10 play an important role in the production of pro-inflammatory cytokines coming from interaction of the intervertebral nucleus pulposus cells and macrophages.
authors.affiliation: Department of Orthopedic Surgery, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
authors.forename: Junya
authors.initials: J
authors.lastname: Yamamoto
authors.forename: Koichiro
authors.initials: K
authors.lastname: Maeno
authors.forename: Toru
authors.initials: T
authors.lastname: Takada
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Kakutani
authors.forename: Takashi
authors.initials: T
authors.lastname: Yurube
authors.forename: Zhongying
authors.initials: Z
authors.lastname: Zhang
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Hirata
authors.forename: Takuto
authors.initials: T
authors.lastname: Kurakawa
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sakai
authors.forename: Joji
authors.initials: J
authors.lastname: Mochida
authors.forename: Minoru
authors.initials: M
authors.lastname: Doita
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kurosaka
authors.forename: Kotaro
authors.initials: K
authors.lastname: Nishida
mesh_terms: D051722:ADAM Proteins / Q000235:genetics / Q000502:physiology*; D000072197:ADAM10 Protein; D053829:Amyloid Precursor Protein Secretases / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000096:biosynthesis*; D053222:Fas Ligand Protein / Q000096:biosynthesis / Q000502:physiology*; D055785:Gene Knockdown Techniques; D006801:Humans; D053583:Interleukin-1beta / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D007403:Intervertebral Disc / Q000166:cytology* / Q000378:metabolism; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D016207:Cytokines; D053222:Fas Ligand Protein; C508600:IL6 protein, human; D053583:Interleukin-1beta; D015850:Interleukin-6; D008565:Membrane Proteins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D053829:Amyloid Precursor Protein Secretases; D051722:ADAM Proteins; D000072197:ADAM10 Protein; C491236:ADAM10 protein, human