title: Effects of the herbal medicine composition "Saiko-ka-ryukotsu-borei-To" on the function of endothelial progenitor cells in hypertensive rats.
abstract: Endothelial progenitor cells (EPCs) are known to repair vascular injuries. Recent studies suggest that Saiko-ka-ryukotsu-borei-To (SKRBT), a traditional herbal medicine that has been used to treat stress-related neuropsychiatric disorders, has protective effects on cardiovascular diseases such as hypertension and arteriosclerosis. Spontaneously hypertensive rats (SHRs) were fed diets containing lyophilized SKRBT extract for 6 weeks. Peripheral blood mononuclear cells (MNCs) were isolated and cultured to assay EPC colony formation. Oxidative stress in MNCs was evaluated by thiobarbituric acid reactive substance (TBARS) assay and flowcytometric analyses. Treatment with SKRBT increased EPC colony numbers significantly (p<0.05) with decrease in oxidative stress and without affecting blood pressure in SHRs. Treatment with SKRBT did not reduce the expression of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase subunits in cardiovascular organs. Serum IL-6 level was significantly reduced. SKRBT is a feasible herbal medicine that protects against cardiovascular diseases through an increase in EPC function along with anti-oxidative effects, and may affect the link between chronic inflammation and cardiovascular disease.
authors.affiliation: School of Pharmacy, Nihon University, Chiba, Japan. iijima.hiroshi@nihon-u.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Iijima
authors.forename: Akihiro
authors.initials: A
authors.lastname: Daikonya
authors.forename: Satoshi
authors.initials: S
authors.lastname: Takamatsu
authors.forename: Ai
authors.initials: A
authors.lastname: Kanno
authors.forename: Kayoko
authors.initials: K
authors.lastname: Magariyama
authors.forename: Kazuki
authors.initials: K
authors.lastname: Yoshikawa
authors.forename: Tomoko
authors.initials: T
authors.lastname: Takamiya
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Ueda
authors.forename: Shuji
authors.initials: S
authors.lastname: Yakubo
authors.forename: Taro
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ueno
authors.forename: Yukio
authors.initials: Y
authors.lastname: Yamori
authors.forename: Noboru
authors.initials: N
authors.lastname: Fukuda
authors.forename: Susumu
authors.initials: S
authors.lastname: Kitanaka
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects*; D016207:Cytokines / Q000097:blood; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D042783:Endothelial Cells / Q000187:drug effects*; D006973:Hypertension / Q000517:prevention & control*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D019255:NADPH Oxidases / Q000378:metabolism; D008517:Phytotherapy; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D017382:Reactive Oxygen Species / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D016207:Cytokines; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; C095444:saiko-ka-ryukotsu-borei-to; D019255:NADPH Oxidases