title: A novel CD105 determination system based on an ultrasensitive bioelectrochemical strategy with Pt nanoparticles.
abstract: CD105 is a well-known tumor metastasis marker and useful for early monitoring of metastasis and cancer relapse. It is important to generate rapid, reliable and precise analytical information regarding CD105 levels. To establish a simple, selective and sensitive detection method, we prepared an immunosensor with novel bioconjugates based on Pt nanoparticles, thionin acetate and antibodies. The proposed immunosensor displayed a broader linear response to CD105, with a working range of 1.3 to 200.0 ng/mL and a detection limit of 0.9 ng/mL under optimal conditions. Moreover, the studied immunosensor exhibited high sensitivity, fast analysis and adequate stability. The proposed methodology could readily be extended to other clinical- or environment-related biospecies.
authors.affiliation: Department of Obstetrics & Gynecology, Southwest Hospital, Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, China. zengsuyun2012@126.com
authors.forename: Suyun
authors.initials: S
authors.lastname: Zeng
authors.forename: Sujun
authors.initials: S
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Lihua
authors.initials: L
authors.lastname: Yang
authors.forename: Zhenbo
authors.initials: Z
authors.lastname: Chen
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Liang
mesh_terms: D015703:Antigens, CD / Q000032:analysis* / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D055088:Early Detection of Cancer / Q000295:instrumentation / Q000379:methods; D055664:Electrochemical Techniques; D000071063:Endoglin; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry; D009362:Neoplasm Metastasis / Q000175:diagnosis*; D009364:Neoplasm Recurrence, Local / Q000175:diagnosis*; D010984:Platinum / Q000737:chemistry; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000276:immunology
chemical_list: D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D011956:Receptors, Cell Surface; D010984:Platinum