title: [Identification of the wild germplasm of Prunus mume based on AFLP markers].
abstract: Mei (Prunus mume Sieb.et Zucc.) is traditionally not only a famous special ornamental plant but also a fruit tree origined in China. In order to conserve and ultilize scientifically the germplasm resources of wild mei, we identified and analysed the germplasm of mei flower in 65 samples collected from the habitat, using AFLP makers in combination with morphological anaylsis. This study amplified clearly 1 728 polymorphic bands, using the 8- pair-primer of Mse I -EcoR I screened totally from 64 -primer combination. According to the Nei' 72 distance coefficient clustering, all of the formas and varieties used in this study, including Prunus mume var. mume, P. mume, P. mume var. goethartiana, P. mume var. pallescens, P. mume var. microcarpa, P. mume var. cenrnus-sempervirens (newly recoeding variety), P. mume var. cernua, P. mume var. pallidus, P. mume var. taomei, were identified at the point of Nei' 72=0.26. Due to the genetic difference obviously among the formas and varieties, we suggested that wild germplasmtypes of all formas and varieties in P. mume should be conserved in their habitats in the furture.
authors.affiliation: China National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China. lqwylxy@sina.com
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Li
authors.forename: Qixiang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Junyu
authors.initials: J
authors.lastname: Chen
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D014644:Genetic Variation; D055993:Germ Cells, Plant / Q000145:classification*; D010802:Phylogeny; D027861:Prunus / Q000145:classification* / Q000235:genetics*; D013045:Species Specificity