title: Infection cycle of Salmonella enterica serovar Enteritidis in latent carrier mice.
abstract: This work reports the distribution of an oral dose of Salmonella enterica serovar Enteritidis (SE) in C57Bl/6-Bcgr mice, to study its pathogenesis in a latent carrier animal. Mice orally inoculated with a high dose of SE developed a latent infection characterized by the absence of clinical symptoms in which the cecum is functioning as a "strategic site" of SE proliferation, releasing bacteria into feces intermittently over the 4-week study. A sequence of disruptions occurred in the small intestine at 1 day postinculation (PI). The microvilli exhibited different degrees of degeneration, which were reversible as the cells became vacuolated. From 2 days PI, SE was detected in the mononuclear phagocytic system, and an exponential growth of the remaining bacteria in tissues was observed until 4 days PI. The production of interferon gamma from 3 days PI is restricting the SE growth, and a plateau phase was observed from 4 to 15 days PI. A recurrence of the bacterial growth in tissue occurred from 15 to 28 days PI, especially in the cecum. Increasing our knowledge about the host-pathogen interaction of adapted pathogens with the ability to develop latency is essential for the development of an efficient strategy for Salmonella control.
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, BR 415, Rod. Ilhéus-Itabuna, Km 16 - Salobrinho, Ilhéus, Bahia 456662-900, Brazil.
authors.forename: Bianca Mendes
authors.initials: BM
authors.lastname: Maciel
authors.forename: Nammalwar
authors.initials: N
authors.lastname: Sriranganathan
authors.forename: Carla Cristina
authors.initials: CC
authors.lastname: Romano
authors.forename: Thalis Ferreira
authors.initials: TF
authors.lastname: dos Santos
authors.forename: João Carlos
authors.initials: JC
authors.lastname: Teixeira Dias
authors.forename: Eduardo
authors.initials: E
authors.lastname: Gross
authors.forename: Rachel Passos
authors.initials: RP
authors.lastname: Rezende
mesh_terms: D000818:Animals; D057138:Bacterial Shedding; D002353:Carrier State / Q000382:microbiology*; D002432:Cecum / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D054884:Host-Pathogen Interactions; D007371:Interferon-gamma / Q000276:immunology; D018664:Interleukin-12 / Q000276:immunology; D007421:Intestine, Small / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D012157:Mononuclear Phagocyte System / Q000276:immunology; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000472:pathogenicity*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D007371:Interferon-gamma