title: Anti-yeast activity of mentha oil and vapours through in vitro and in vivo (real fruit juices) assays.
abstract: The anti-yeast activity of mentha oil and vapours was evaluated against 8 food spoiling yeasts through disc diffusion, disc volatilisation and micro broth dilution method. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) varied from 0.28 to 2.25 and 1.13 to 4.5 mg/ml, respectively. Furthermore, the anti-yeast efficacy of mentha oil alone and in combination with thermal treatment was evaluated in a real food system i.e. mixed fruit juices. The samples treated with a combination of mentha oil at the MIC, ½ MIC and ¼ MIC levels and thermal treatment enhanced the reduction viability. Chemical characterisation of mentha oil by gas chromatography-mass spectrometry (GC-MS) revealed that the dominant compounds were cis-menthone (27.43%), menthol (24.3%), trans-menthone (9.23%), limonene (5.84%), menthofuran (4.44%) and isomenthol (3.21%). Present results established the superior performance of integrated treatment over individual exposure for fruit juice preservation.
authors.affiliation: Applied Microbiology Laboratory, Centre for Rural Development & Technology, Indian Institute of Technology Delhi, New Delhi 110016, India. amittyagiiitd@gmail.com
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Tyagi
authors.forename: Davide
authors.initials: D
authors.lastname: Gottardi
authors.forename: Anushree
authors.initials: A
authors.lastname: Malik
authors.forename: Maria Elisabetta
authors.initials: ME
authors.lastname: Guerzoni
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D001628:Beverages / Q000032:analysis / Q000382:microbiology*; D005519:Food Preservation; D005520:Food Preservatives / Q000737:chemistry / Q000494:pharmacology*; D027541:Mentha / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*; D015003:Yeasts / Q000187:drug effects / Q000254:growth & development
chemical_list: D000935:Antifungal Agents; D005520:Food Preservatives; D009822:Oils, Volatile; D010938:Plant Oils