title: [The assessment of selected molecules belonging to B7 family on the mature dendritic cells in laryngeal cancer patients].
abstract: B7-H1, B7-H2 and B7-H4 molecules play various roles in the adaptive immune system and are broadly expressed on many cells, especially those localized in cancer tissue. The aim of the study was to assess the surface expression of B7-H1, B7-H2 and B7-H4 molecules on the mature dendritic cells (DC) generated from peripheral blood monocytes of laryngeal cancer patients and healthy donors.
  
  
  METHODS
  Forty-four male patients treated surgically for squamous cell carcinoma of the larynx were included in the study. Peripheral blood from twelve healthy male donors was used as a control. Mononuclear cells were separated from all individuals by density gradient centrifugation, incubated with anti-CD14 microbeads, and passed through MACS separation columns. The CD14 positive cell population was used to prepare monocyte derived DC. Laryngeal cancer tissue was obtained from patients during surgical treatment and homogenized to prepare tumor cell lysates for further stimulation. We evaluated with the use of flow cytometry method the percentage of cells with an expression and mean fluorescent intensity (MFI) of surface markers, such as: CD83, B7-H1, B7-H2, B7-H4.
  
  
  RESULTS
  Our study revealed that the percentage of mature dendritic cells, stimulated with autologous tumor cell lysates, with the expression of B7-H1 molecule in patients was lower than in healthy donors (61.81 ± 25.58% vs 93.02 ± 4.63%, p=0.007). In laryngeal cancer patients, the percentage of CD83+/B7-H2+ cells was higher than in healthy individuals (18.32 ± 10.74% vs 2.89 ± 0.43%, p=0.019).
  
  
  CONCLUSIONS
  There is a relation between the presence of laryngeal cancer and the expression of B7 family molecules.
authors.affiliation: Katedra i Klinika Otolaryngologii i Onkologii Laryngologicznej Uniwersytetu Medycznego w Lublinie, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Klatka
authors.forename: Ewelina
authors.initials: E
authors.lastname: Grywalska
authors.forename: Magdalena
authors.initials: M
authors.lastname: Wasiak
authors.forename: Paulina
authors.initials: P
authors.lastname: Wdowiak
authors.forename: Adrian
authors.initials: A
authors.lastname: Andrzejczak
authors.forename: Eliza
authors.initials: E
authors.lastname: Trzaskowska
authors.forename: Jacek
authors.initials: J
authors.lastname: Roliński
mesh_terms: D060887:B7 Antigens / Q000378:metabolism*; D018122:B7-1 Antigen / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism*; D003713:Dendritic Cells / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D060945:Inducible T-Cell Co-Stimulator Ligand / Q000378:metabolism; D007822:Laryngeal Neoplasms / Q000276:immunology* / Q000473:pathology*; D008297:Male; D060907:V-Set Domain-Containing T-Cell Activation Inhibitor 1 / Q000378:metabolism
chemical_list: D060887:B7 Antigens; D018122:B7-1 Antigen; D014408:Biomarkers, Tumor; C498514:ICOSLG protein, human; D060945:Inducible T-Cell Co-Stimulator Ligand; D060907:V-Set Domain-Containing T-Cell Activation Inhibitor 1; C475826:VTCN1 protein, human