title: Putative circulating markers of the early and advanced stages of breast cancer identified by high-resolution label-free proteomics.
abstract: This study evaluated the plasmatic proteomic profile of breast cancer patients in the early (ED) and advanced (AD) stages, employing high-throughput proteomics. We identified 92 differentially expressed proteins in ED and 73 proteins in AD patients. Gelsolin, lumican, clusterin, SALL4 and PMS2, as well hTERT, TNF-α and GRHL3 were chosen for further investigation. ED presented augmented expression of GRHL3 and reduced circulating TNF-α with high expression of GRHL3 in tumors. AD displayed high TNF-α and a significant expression of PMS2 in tumors. These findings suggest processes enrolling stem cell division in ED, with TNF-α signaling and DNA mismatch repair in the advanced stage.
authors.affiliation: Laboratório de Células Tronco, Instituto Nacional do Câncer, INCA, Rio de Janeiro, Brazil. carolpanis@sercomtel.com.br
authors.forename: Carolina
authors.initials: C
authors.lastname: Panis
authors.forename: Luciana
authors.initials: L
authors.lastname: Pizzatti
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Herrera
authors.forename: Rubens
authors.initials: R
authors.lastname: Cecchini
authors.forename: Eliana
authors.initials: E
authors.lastname: Abdelhay
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D001943:Breast Neoplasms / Q000097:blood* / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000097:blood*; D009367:Neoplasm Staging; D011446:Prospective Studies; D040901:Proteomics / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins