title: Enhancement of the selective enzymatic biotransformation of rutin to isoquercitrin using an ionic liquid as a co-solvent.
abstract: An ionic liquid (IL)-containing buffer system was first applied in the conversion of rutin to isoquercitrin. High substrate solubility was achieved to enhance the selectivity and efficiency of hesperidinase-catalyzed reaction. Ten ILs were selected as co-solvents to assist catalytic reactions in this biotransformation process. The transformed products of rutin were identified by LC-MS. The [Bmim][BF(4)]-glycine-sodium hydroxide buffer (pH 9) (10:90, v/v) was found to be the best medium for the biotransformation of isoquercitrin from rutin with higher selectivity and efficiency. The reaction time was reduced by 0.33-fold while the conversion of rutin and the yield of isoquercitrin were increased by 1.67-fold and 2.33-fold. The results suggest that IL co-solvents have great potential to enhance the selectively enzymatic hydrolysis of rutin for isoquercitrin production.
authors.affiliation: School of Biology and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Guo-Xia
authors.initials: GX
authors.lastname: Sun
authors.forename: Liang
authors.initials: L
authors.lastname: Yu
authors.forename: Fu-An
authors.initials: FA
authors.lastname: Wu
authors.forename: Xi-Jie
authors.initials: XJ
authors.lastname: Guo
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D001711:Biotransformation; D006026:Glycoside Hydrolases / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000302:isolation & purification; D012431:Rutin / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*
chemical_list: D052578:Ionic Liquids; D012997:Solvents; C016527:isoquercitrin; D012431:Rutin; D011794:Quercetin; D006026:Glycoside Hydrolases; C023307:hesperidinase