title: Early signaling events in grapevine cells elicited with cyclodextrins and methyl jasmonate.
abstract: The use of cyclic oligosaccharides like cyclodextrins (CDs), alone or combined with methyl jasmonate (MJ), as elicitors has proved very effective in stimulating the production of trans-resveratrol (trans-R) in Vitis vinifera suspension-cultured cells (SCC). Since elicitors can be used to increase trans-R production, understanding the molecular mechanisms involved would improve the management of grapevine cells as factories of this compound. The results obtained in this study provide evidence for a role of Ca(2+) in mediating elicitor-induced trans-R production in grapevine SCC. The Ca(2+) elevation was promoted by an uptake of Ca(2+) from the extracellular medium, and by Ca(2+) mobilization from intracellular organelles. Moreover, protein phosphorylation/dephosphorylation events seem to be involved in the signal transduction pathways triggered by CDs separately or in combination with MJ since trans-R production is dependent on both, the phosphorylation status of several proteins through mitogen-activated kinase pathway and the activity of tyrosine phosphatases. Our results also suggest that H(2)O(2) and NO participated in the production of trans-R triggered by both elicitors in grapevine SCC. Finally, a fast alkalinization of the extracellular medium is induced in the presence of CDs and/or MJ.
authors.affiliation: Department of Plant Biology, Faculty of Biology, University of Murcia, Campus de Espinardo, E-30100 Murcia, Spain.
authors.forename: Sarai
authors.initials: S
authors.lastname: Belchí-Navarro
authors.forename: Lorena
authors.initials: L
authors.lastname: Almagro
authors.forename: Ana Belén
authors.initials: AB
authors.lastname: Sabater-Jara
authors.forename: Francisco
authors.initials: F
authors.lastname: Fernández-Pérez
authors.forename: Roque
authors.initials: R
authors.lastname: Bru
authors.forename: Maria A
authors.initials: MA
authors.lastname: Pedreño
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D020013:Calcium Signaling / Q000187:drug effects* / Q000502:physiology; D003505:Cyclodextrins / Q000494:pharmacology*; D003517:Cyclopentanes / Q000494:pharmacology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects* / Q000502:physiology; D009569:Nitric Oxide / Q000378:metabolism; D054883:Oxylipins / Q000494:pharmacology*; D059828:Plant Cells / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000378:metabolism; D027843:Vitis / Q000166:cytology / Q000378:metabolism*
chemical_list: D000085:Acetates; D003505:Cyclodextrins; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D009569:Nitric Oxide; C072239:methyl jasmonate; D006861:Hydrogen Peroxide; D048049:Extracellular Signal-Regulated MAP Kinases