title: Quantization of Dextromethorphan and Levocetirizine in Combined Dosage form Using a Novel Validated RP-HPLC Method.
abstract: The present study reveals a simple isocratic RP-HPLC method for the simultaneous determination of dextromethorphan hydrobromide and levocetirizine dihydrochloride in a cough syrup. The separation of these compounds was achieved within 10 min on a Phenomenex (USA) C(18) analytical column, 250×4.0 mm i.d., using an isocratic mobile phase consisting of potassium dihydrogen phosphate buffer (pH 2.5) - acetonitrile- tetrahydrofuran (70:25:5, v/v/v). The analysis was performed at a flow rate of 1.2 ml/min and at a detection wavelength of 232 nm. Percentage recovery and RSD were 100.36% and 0.05% for levocetirizine dihydrochloride, 100.35% and 0.27% for dextromethorphan hydrobromide respectively. Quantification of the components in syrup formulation was calculated against the peak areas of freshly prepared standard solutions. The method was validated as per ICH guidelines.
authors.affiliation: Department of Environmental Studies, School of Environmental Studies and Natural Resources, Doon University, Dehradun-248 001, India.
authors.forename: Shalini
authors.initials: S
authors.lastname: Joshi
authors.forename: C
authors.initials: C
authors.lastname: Bhatia
authors.forename: C S
authors.initials: CS
authors.lastname: Bal
authors.forename: M S M
authors.initials: MS
authors.lastname: Rawat