title: Effect of various stress-regulatory factors on biomass and lipid production in microalga Haematococcus pluvialis.
abstract: To maximize the biomass and lipid production for applications in food or biofuel feedstock, nine stress conditions were tested considering N and/or P limitations, light intensity & quality, for Haematococcus pluvialis SCCAP K-0084 cultivation. Photosynthetically active radiation (PAR), warm white light emitting diode (WWLED), and white light emitting diode (WLED) at illumination of 240 μmol photons m(-2) sec(-1) were the best stress-regulatory factors. PAR without P & low N conditions yielded high biomass with 33% lipids containing increased C16:0 and C18:0 saturated fatty acids, and reduced unsaturated fatty acids (UFAs) (oleic, linoleic, and α/γ-linolenic). WWLED and WLED without P conditions also yielded high biomass, but 25% lipids with increased amounts of UFAs. Red light emitting diode (RLED) without P & low N conditions yielded 46% lipids with lowest biomass. PAR and WWLED & WLED illuminated conditions were found suitable respectively for biodiesel feedstock lipids and UFA-rich lipids for multiple applications.
authors.affiliation: Shannon Applied Biotechnology Centre, Limerick Institute of Technology, Moylish Park, Limerick, Ireland. Sushanta.saha@lit.ie
authors.forename: Sushanta Kumar
authors.initials: SK
authors.lastname: Saha
authors.forename: Edward
authors.initials: E
authors.lastname: McHugh
authors.forename: Jeremiah
authors.initials: J
authors.lastname: Hayes
authors.forename: Siobhan
authors.initials: S
authors.lastname: Moane
authors.forename: Daniel
authors.initials: D
authors.lastname: Walsh
authors.forename: Patrick
authors.initials: P
authors.lastname: Murray
mesh_terms: D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D049109:Cell Proliferation; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000145:classification / Q000502:physiology*; D013045:Species Specificity; D013312:Stress, Physiological / Q000502:physiology*
chemical_list: D008055:Lipids