title: The association of interleukin-16 gene polymorphisms with susceptibility of coronary artery disease.
abstract: OBJECTIVE
  Recent studies have shown that interleukin (IL)-16 is an immunomodulatory cytokine, which plays an important role in some inflammatory and autoimmune diseases. We aimed to investigate the association between the IL-16 gene polymorphisms and presence of coronary artery disease (CAD) where inflammatory processes are involved.
  
  
  METHODS
  This case-control study enrolled 651 CAD patients confirmed by coronary angiography and 428 controls. Four tag single nucleotide polymorphisms (rs8034928-rs3848180-rs4577037-rs1131445) within the IL-16 gene and the related haplotypes were genotyped by using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). Plasma IL-16 concentration was measured by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  In patients with CAD, the plasma concentration of IL-16 was significantly higher than in controls (97.6±10.7, 66.5±9.6, respectively P<0.001). By using multivariate logistic regression analysis, the allele and genotype frequencies of rs8034928 were different between CAD and control groups (P<0.001). However, the associations of the polymorphisms rs3848180, rs4577037, and rs1131445 with CAD were not observed. The haplotypes TTTT and TGGT significantly increased risk to CAD (OR, 95% CI: 1.43, 1.26-1.63; 1.47, 1.16-1.85; respectively), whereas the haplotypes CTTT and TTGT referred to protection of CAD (OR, 95% CI: 0.45, 0.33-0.62; 0.50, 0.33-0.76; respectively).
  
  
  CONCLUSIONS
  The study indicated that the IL-16 rs8034928 T/C polymorphism and haplotypes were associated with the presence of CAD in Chinese Han population. The IL-16 gene polymorphisms may be a useful predictor to the susceptibility of CAD.
authors.affiliation: Department of Cardiology, West China Hospital, Sichuan University, 610041 Chengdu, Sichuan, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Huang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zeng
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.forename: Ou
authors.initials: O
authors.lastname: Qiang
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000368:Aged; D000483:Alleles; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D017023:Coronary Angiography; D003324:Coronary Artery Disease / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies / Q000379:methods; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease / Q000208:ethnology; D006239:Haplotypes; D006801:Humans; D019410:Interleukin-16 / Q000097:blood / Q000235:genetics*; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: D005819:Genetic Markers; D019410:Interleukin-16