title: Development of a SYBR green real-time PCR method for rapid detection of sheep pox virus.
abstract: BACKGROUND
  In this study, we developed a SYBR Green-based real-time PCR assay for the detection of sheep pox virus using a plasmid construct carrying one of the highly conserved genes encoding the virion envelope protein (P32) as a template.
  
  
  RESULTS
  The method was demonstrated to be highly sensitive, allowing a precise SPV DNA quantitation over a range of nine orders of magnitude (from 101 to 109 copies of standard DNA). Then, specimens from SPV suspected sheep were analyzed by conventional gel-based PCR, real-time PCR and sequence analysis.
  
  
  CONCLUSIONS
  Comparison between these different techniques revealed that real-time PCR is more sensitive than conventional gel-based PCR, allowing detection low viral titers of SPV in infected sheep.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou 730046, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Tian
authors.forename: Jingyan
authors.initials: J
authors.lastname: Wu
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Keshan
authors.initials: K
authors.lastname: Zhang
authors.forename: Youjun
authors.initials: Y
authors.lastname: Shang
authors.forename: Xiangtao
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D018151:Capripoxvirus / Q000235:genetics / Q000302:isolation & purification*; D011213:Poxviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D060888:Real-Time Polymerase Chain Reaction; D012016:Reference Values; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis*; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins