title: Polyhydroxyalkanoate production from fermented volatile fatty acids: effect of pH and feeding regimes.
abstract: The combined fermentation of sludge from the secondary sedimentation tank of municipal wastewater treatment plants and food waste enables not only waste reduction, but also the acquisition of volatile fatty acids (VFAs) for the biosynthesis of polyhydroxyalkanoates (PHAs). To better understand variables influencing the production of VFAs and PHAs, this study considered anaerobic fermentation of VFAs under different pH conditions. The production of VFA was the highest at an initial pH of 9.0, reaching 25,934 ± 1,485 mg COD/L and a VFAs/S COD ratio of 0.61 ± 0.04. When the fermentation liquid was used as a carbon source for PHA biosynthesis, continuous pulsed feeding resulted in the highest PHA synthesis rate of 64.5 ± 1.8%, while the culture receiving a one-time feeding had the lowest rate of only 51.5 ± 2.0%.
authors.affiliation: Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China. chen_hong@zju.edu.cn
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Huijuan
authors.initials: H
authors.lastname: Meng
authors.forename: Zuchao
authors.initials: Z
authors.lastname: Nie
authors.forename: Mingmei
authors.initials: M
authors.lastname: Zhang
mesh_terms: D001421:Bacteria, Anaerobic / Q000737:chemistry* / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000378:metabolism*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis* / Q000302:isolation & purification; D012722:Sewage / Q000382:microbiology*
chemical_list: D003470:Culture Media; D005232:Fatty Acids, Volatile; D054813:Polyhydroxyalkanoates; D012722:Sewage