title: Clinical significance and expression of PAF and TNF-alpha in seminal plasma of leukocytospermic patients.
abstract: OBJECTIVE
  Discuss the changes and roles of PAF in the reproductive tract infection by observing the expression of platelet activating factor (PAF) and tumor necrosis factor α (TNF-α) in seminal plasma of patients with leukocytospermia.
  
  
  METHODS
  The seminal plasma was obtained from 22 cases of leukocytospermia and 15 cases of normal males; the peroxidase dyeing method was adopted for seminal plasma white blood count; the ELISA was adopted to test PAF and TNF-α concentration in seminal plasma.
  
  
  RESULTS
  PAF concentration (2.14 ± 0.43 ng/mL) of leukocytospermia group was significantly lower than the normal group (6.21 ± 1.38 ng/mL, P < 0.01) while TNF-α (5.51 ± 1.46 ng/mL) was significantly higher than that of normal group (3.48 ± 1.08 ng/mL). There was negative correlation between PAF and TNF-α , (r = -0.68, P < 0.01); the same situation existed in PAF and WBC (r = -0.62, P < 0.01); but TNF-α was positively correlated to WBC (r = 0.77, P < 0.01).
  
  
  CONCLUSIONS
  (1) Low expression of PAF and high expression of TNF-α in leukocytospermia affect the sperm motility, which is one of the reasons that leads to infertility. (2) Lower expression of PAF has its particularity during the reproductive tract infection.
authors.affiliation: Department of Urology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China. chfliu315@163.com
authors.forename: Chaodong
authors.initials: C
authors.lastname: Liu
authors.forename: Hongjian
authors.initials: H
authors.lastname: Liu
authors.forename: Xianzhong
authors.initials: X
authors.lastname: Wang
authors.forename: Sun
authors.initials: S
authors.lastname: Xinbo
mesh_terms: D000328:Adult; D006801:Humans; D007248:Infertility, Male / Q000209:etiology*; D007958:Leukocyte Count; D007964:Leukocytosis / Q000276:immunology*; D008297:Male; D010972:Platelet Activating Factor / Q000032:analysis*; D012661:Semen / Q000737:chemistry* / Q000166:cytology; D013081:Sperm Motility; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
chemical_list: D010972:Platelet Activating Factor; D014409:Tumor Necrosis Factor-alpha