title: Dynamic regulation of bone morphogenetic proteins in engineered osteochondral constructs by biomechanical stimulation.
abstract: Osteochondral tissue-engineered grafts are proposed to hold greater potential to repair/regenerate damaged cartilage through enhanced biochemical and mechanical interactions with underlying subchondral bone as compared to simple engineered cartilage. Additionally, biomechanical stimulation of articular chondrocytes (ACs) or osteoblasts (OBs) was shown to induce greater morphogenesis of the engineered tissues composed of these cells. In this report, to define the advantages of biomechanical stimulation to osteochondral grafts for tissue engineering, we examined whether (1) ACs and OBs in three-dimensional (3D) osteochondral constructs support functional development of each other at the molecular level, and (2) biomechanical stimulation of osteochondral constructs further promotes the regenerative potential of such grafts. Various configurations of cell/scaffold assemblies, including chondral, osseous, and osteochondral constructs, were engineered with mechano-responsive electrospun poly(ɛ-caprolactone) scaffolds. These constructs were subjected to either static or dynamic (10% cyclic compressive strain at 1 Hz for 3 h/day) culture conditions for 2 weeks. The expression of bone morphogenetic proteins (BMPs) was examined to assess the regenerative potential of each treatment on the cells. Biomechanical stimulation augmented a marked upregulation of Bmp2, Bmp6, and Bmp7 as well as downregulation of BMP antagonist, Bmp3, in a time-specific manner in the ACs and OBs of 3D osteochondral constructs. More importantly, the presence of biomechanically stimulated OBs was especially crucial for the induction of Bmp6 in ACs, a BMP required for chondrocytic growth and differentiation. Biomechanical stimulation led to enhanced tissue morphogenesis possibly through this BMP regulation, evident by the improved effective compressive modulus of the osteochondral constructs (710 kPa of dynamic culture vs. 280 kPa of static culture). Similar BMP regulation was observed in the femoral cartilages of the rats subjected to gentle exercise, demonstrating the physiological relevance of in vitro biomechanical stimulation of osteochondral constructs. Overall, our findings show that biomechanical stimulation may be critical for cross signaling between ACs and OBs to support chondrocytic growth in 3D osteochondral tissues.
authors.affiliation: Department of Bioengineering, University of California, Riverside, California 92521, USA. jnam@engr.ucr.edu
authors.forename: Jin
authors.initials: J
authors.lastname: Nam
authors.forename: Priyangi
authors.initials: P
authors.lastname: Perera
authors.forename: Bjoern
authors.initials: B
authors.lastname: Rath
authors.forename: Sudha
authors.initials: S
authors.lastname: Agarwal
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena / Q000187:drug effects; D019485:Bone Morphogenetic Proteins / Q000378:metabolism* / Q000494:pharmacology; D002358:Cartilage, Articular / Q000166:cytology; D019902:Chondrocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D019245:Compressive Strength / Q000187:drug effects; D005260:Female; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D019899:Paracrine Communication / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D019485:Bone Morphogenetic Proteins