title: Rhizobium tarimense sp. nov., isolated from soil in the ancient Khiyik River.
abstract: A Gram-negative, non-motile, pale-yellow, rod-shaped bacterial strain, PL-41(T), was isolated from Populus euphratica forest soil at the ancient Khiyik River valley in Xinjiang Uyghur Autonomous Region, People's Republic of China. Strain PL-41(T) grew optimally at 30 °C and pH 7.0-8.0. The major quinone was Q-10. The predominant cellular fatty acids of strain PL-41(T) were summed feature 8 (comprising C18 : 1ω7c and C18 : 1ω6c), C16 : 0 and C19 : 0 cyclo ω8c. Polar lipids of strain PL-41(T) include two unidentified aminophospholipids (APL1, 2), two unidentified phospholipids (PL1, 2), phosphatidylcholine and three unidentified lipids (L1-3). Strain PL-41(T) showed 16S rRNA gene sequence similarity of 97.0-97.5 % to the type strains of recognized species of the genus Rhizobium. Phylogenetic analysis of strain PL-41(T) based on the sequences of housekeeping genes recA and atpD confirmed (similarities are less than 90 %) its position as a distinct species of the genus Rhizobium. The DNA G+C content was 57.8 mol%. DNA-DNA relatedness between strain PL-41(T) and the type strains of Rhizobium huautlense S02(T), Rhizobium alkalisoli CCBAU 01393(T), Rhizobium vignae CCBAU 05176(T) and Rhizobium loessense CCBAU 7190B(T) were 33.4, 22.6, 25.5 and 45.1 %, respectively, indicating that strain PL-41(T) was distinct from them genetically. Strain PL-41(T) also can be differentiated from these four phylogenetically related species of the genus Rhizobium by various phenotypic properties. On the basis of phenotypic properties, phylogenetic distinctiveness and genetic data, strain PL-41(T) is considered to represent a novel species of the genus Rhizobium, for which the name Rhizobium tarimense sp. nov. is proposed. The type strain is PL-41(T) ( = CCTCC AB 2011011(T) = NRRL B-59556(T)).
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors.forename: Maripat
authors.initials: M
authors.lastname: Turdahon
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors.forename: Ghenijan
authors.initials: G
authors.lastname: Osman
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors.forename: Maryam
authors.initials: M
authors.lastname: Hamdun
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors.forename: Khayir
authors.initials: K
authors.lastname: Yusuf
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors.forename: Zumret
authors.initials: Z
authors.lastname: Abdurehim
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors.forename: Gulsumay
authors.initials: G
authors.lastname: Abaydulla
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors.forename: Muhtar
authors.initials: M
authors.lastname: Abdukerim
authors.affiliation: College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Chengxiang
authors.initials: C
authors.lastname: Fang
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumchi, Xinjiang 830046, PR China.
authors.forename: Erkin
authors.initials: E
authors.lastname: Rahman
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D045483:Rivers; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C024989:coenzyme Q10