title: Substrate utilization of stress tolerant methylotrophs isolated from revegetated heavy metal polluted coalmine spoil.
abstract: We analyzed methylotrophs in Bina natural vegetation (BNV), and revegetated overburden dump of four (ROBD4) and 12 years (ROBD12), at Bina coal mine in Sonbhadra district. The cultured strains identified as Pseudomonas, Acinetobacter, Stenotrophomonas and Cellvibrio (γ-Proteobacteria), Methylophilus, Ralstonia, Burkholderia (α-Proteobacteria) Methylobacterium and Inquilinus (β-Proteobacteria), Bacillus (Firmicutes) and Flexibacter (Sphingobacteria) in their 16s rRNA gene sequence similarity. The strains differed in citrate, lactose, formate, urea and xylose utilization. Methanol utilization by Stenotrophomonas, Inquilinus, Cellvibrio and Flexibacter is for first time. The preferred N- sources were proline, glutamate and nitrate for most of the strains. All strains tolerated (2.5 % NaCl) and SDS (0.2 %); 16 strains survived in crystal violet (0.01 %) and nine strains in sodium azide (0.02 %. Methylotrophic population trend was BNV > ROBD12 > ROBD4. The presence of majority of strain of BNV at ROBD12 and ROBD4 indicated restoration of soil methylotrophic functional diversity in revegetated dumps.
authors.affiliation: Department of Chemical Engineering and Technology, Institute of Technology, Banaras Hindu University, Varanasi 221005, India.
authors.forename: D D
authors.initials: DD
authors.lastname: Giri
authors.forename: P N
authors.initials: PN
authors.lastname: Shukla
authors.forename: Singh
authors.initials: S
authors.lastname: Ritu
authors.forename: Ajay
authors.initials: A
authors.lastname: Kumar
authors.forename: K D
authors.initials: KD
authors.lastname: Pandey
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000302:isolation & purification* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D044822:Biodiversity; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D009930:Organic Chemicals / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009930:Organic Chemicals; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants