title: [Enhanced cellulase production of Penicillium decumbens by knocking out CreB encoding a deubiquitination enzyme].
abstract: Penicillium decumbens T. is an important filamentous fungus for the production of cellulases to effectively degrade lignocellulose for second generation biofuel production. In order to enhance the capability of Penicillium decumbens to produce cellulases, we constructed a creB (a deubiquitinating enzyme encoding gene) deletion cassette, and generated a creB knockout strain with homologous double crossover recombination. This mutation resulted in a detectable decrease of carbon catabolite repression (CCR) effect. The filter paper activity, endoglucanase activity, xylanase activity and exoglucanase activity of the deltacreB strain increased by 1.8, 1.71, 2.06 and 2.04 fold, respectively, when comparing with the parent strain Ku-39. A 2.68 fold increase of extracellular protein concentration was also observed. These results suggest that the deletion of creB results in CCR derepression. These data also suggest that CREB influences cellulase production of Penicillium decumbens. In generation, this study provides information that can be helpful for constructing cellulase hyper-producing strain.
authors.affiliation: School of Bioengineering, Dalian Polytechnic University, Dalian 116034, Liaoning, China.
authors.forename: Guangqi
authors.initials: G
authors.lastname: Zhou
authors.forename: Jing
authors.initials: J
authors.lastname: Lü
authors.forename: Zhonghai
authors.initials: Z
authors.lastname: Li
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.forename: Mingyu
authors.initials: M
authors.lastname: Wang
authors.forename: Yinbo
authors.initials: Y
authors.lastname: Qu
authors.forename: Lin
authors.initials: L
authors.lastname: Xiao
authors.forename: Shulin
authors.initials: S
authors.lastname: Qin
authors.forename: Haitao
authors.initials: H
authors.lastname: Zhao
authors.forename: Ruirui
authors.initials: R
authors.lastname: Xia
authors.forename: Xu
authors.initials: X
authors.lastname: Fang
mesh_terms: D002480:Cellulase / Q000096:biosynthesis*; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D055786:Gene Knockout Techniques; D008031:Lignin / Q000378:metabolism; D050505:Mutant Proteins / Q000378:metabolism*; D010407:Penicillium / Q000201:enzymology / Q000235:genetics*; D011995:Recombination, Genetic; D057149:Ubiquitinated Proteins / Q000235:genetics*; D054875:Ubiquitination
chemical_list: D050505:Mutant Proteins; D057149:Ubiquitinated Proteins; C036909:lignocellulose; D008031:Lignin; D002480:Cellulase; D010450:Endopeptidases