title: Simultaneous detection of biomolecular interactions and surface topography using photonic force microscopy.
abstract: We developed a photonic force microscope that can map multiple parameters simultaneously, including the surface topography and biomolecular interactions. To track the position of the probe bead and to determine contact position with the sample surface, we adopted a video analysis method using the diffraction pattern of monochromatic light passing through the probe bead. To demonstrate the capability of the microscope, we report the simultaneous measurement of the molecule distribution of DNA oligonucleotides on the surface, the binding strength of DNA hybridization between the bead and surface, and the topography of the smooth molded surface.
authors.affiliation: Graduate School of Nanoscience & Technology-WCU, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: Seungjin
authors.initials: S
authors.lastname: Heo
authors.forename: Kipom
authors.initials: K
authors.lastname: Kim
authors.forename: Rodriguez
authors.initials: R
authors.lastname: Christophe
authors.forename: Tae-Young
authors.initials: TY
authors.lastname: Yoon
authors.forename: Yong-Hoon
authors.initials: YH
authors.lastname: Cho
mesh_terms: D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D018625:Microscopy, Atomic Force / Q000379:methods*; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry / Q000302:isolation & purification*; D017785:Photons; D013499:Surface Properties
chemical_list: D009841:Oligonucleotides; D004247:DNA