title: Prevalence and characterization of foodborne pathogens in dairy cattle in the eastern part of Japan.
abstract: To investigate the prevalence and characterization of foodborne pathogens [Campylobacter spp., Shiga toxin-producing Escherichia coli (STEC), Listeria monocytogenes and Salmonella spp.] in dairy cows, rectal content grab samples were collected from 250 dairy cows reared on 25 dairy farms in eastern Japan from December 2010 through February 2011. Campylobacter jejuni was isolated from 106 (42%) cows on 23 (92%) farms, STEC O157 from three cows on one farm, L. monocytogenes from three cows on another three farms and Salmonella enterica subsp. enterica serovar Typhimurium from eight cows on another farm. STEC O26 was not isolated from any of the dairy farms investigated. The results suggest that C. jejuni is widespread in dairy farms in eastern Japan.
authors.affiliation: Food Safety and Consumer Affairs Bureau, Ministry of Agriculture, Forestry and Fisheries, 1-2-1 Kasumigaseki, Chiyoda-ku, Tokyo, Japan. yoshimasa_sasaki@nm.maff.go.jp
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Sasaki
authors.forename: Mariko
authors.initials: M
authors.lastname: Murakami
authors.forename: Mika
authors.initials: M
authors.lastname: Haruna
authors.forename: Noriko
authors.initials: N
authors.lastname: Maruyama
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mori
authors.forename: Kazuo
authors.initials: K
authors.lastname: Ito
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Yamada
mesh_terms: D000818:Animals; D016123:Campylobacter jejuni / Q000235:genetics / Q000302:isolation & purification*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005260:Female; D007564:Japan / Q000453:epidemiology; D008089:Listeria monocytogenes / Q000235:genetics / Q000302:isolation & purification*; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D012475:Salmonella / Q000235:genetics / Q000302:isolation & purification*; D054323:Shiga-Toxigenic Escherichia coli / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial