title: Trypanosomatid pin1-type peptidyl-prolyl isomerase is cytosolic and not essential for cell proliferation.
abstract: Pin1-type peptidyl-prolyl cis/trans isomerases (PPIases) isomerise the peptide bond of specific phosphorylated (Ser/Thr)-Pro residues, regulating various cellular events. Previously, we reported a Pin1-type PPIase in Trypanosoma cruzi, but little is known about its function and subcellular localization. Immunofluorescence analysis revealed that in contrast with Pin1-like proteins from diverse organisms, TcPin1 mainly localized in the cytoplasm and was excluded from the nuclei. In addition, RNAi-mediated downregulation of TbPin1 in Trypanosoma brucei did not abolish cell proliferation. Using yeast two-hybrid assay, we identified a MORN domain-containing protein as putative Pin1-binding partners. These data suggest that Pin1-mediated signaling mechanism plays a different role in protozoan parasites.
authors.affiliation: Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Hector N. Torres" (INGEBI-CONICET), Vuelta de Obligado 2490, C1428ADN, Buenos Aires, R. Argentina. e.erben@zmbh.uni-heidelberg.de
authors.forename: Esteban D
authors.initials: ED
authors.lastname: Erben
authors.forename: Sheila C
authors.initials: SC
authors.lastname: Nardelli
authors.forename: Teresa C L
authors.initials: TC
authors.lastname: de Jesus
authors.forename: Sergio
authors.initials: S
authors.lastname: Schenkman
authors.forename: Maria T
authors.initials: MT
authors.lastname: Tellez-Iñon
mesh_terms: D002453:Cell Cycle; D005786:Gene Expression Regulation; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D019696:Peptidylprolyl Isomerase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014162:Transfection; D014346:Trypanosoma brucei brucei / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D014349:Trypanosoma cruzi; D020798:Two-Hybrid System Techniques
chemical_list: D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D019696:Peptidylprolyl Isomerase