title: Biofuel components change the ecology of bacterial volatile petroleum hydrocarbon degradation in aerobic sandy soil.
abstract: We tested the hypothesis that the biodegradation of volatile petroleum hydrocarbons (VPHs) in aerobic sandy soil is affected by the blending with 10 percent ethanol (E10) or 20 percent biodiesel (B20). When inorganic nutrients were scarce, competition between biofuel and VPH degraders temporarily slowed monoaromatic hydrocarbon degradation. Ethanol had a bigger impact than biodiesel, reflecting the relative ease of ethanol compared to methyl ester biodegradation. Denaturing gradient gel electrophoresis (DGGE) of bacterial 16S rRNA genes revealed that each fuel mixture selected for a distinct bacterial community, each dominated by Pseudomonas spp. Despite lasting impacts on soil bacterial ecology, the overall effects on VHP biodegradation were minor, and average biomass yields were comparable between fuel types, ranging from 0.40 ± 0.16 to 0.51 ± 0.22 g of biomass carbon per gram of fuel carbon degraded. Inorganic nutrient availability had a greater impact on petroleum hydrocarbon biodegradation than fuel composition.
authors.affiliation: School of Civil Engineering and Geosciences, Newcastle University, Newcastle upon Tyne NE1 7RU, England, United Kingdom.
authors.forename: Abdulmagid
authors.initials: A
authors.lastname: Elazhari-Ali
authors.forename: Arvind K
authors.initials: AK
authors.lastname: Singh
authors.forename: Russell J
authors.initials: RJ
authors.lastname: Davenport
authors.forename: Ian M
authors.initials: IM
authors.lastname: Head
authors.forename: David
authors.initials: D
authors.lastname: Werner
mesh_terms: D000332:Aerobiosis; D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D056804:Biofuels / Q000032:analysis; D006838:Hydrocarbons / Q000032:analysis / Q000378:metabolism*; D010578:Petroleum / Q000032:analysis / Q000378:metabolism*; D012822:Silicon Dioxide / Q000737:chemistry; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D012989:Soil Pollutants
chemical_list: D056804:Biofuels; D006838:Hydrocarbons; D010578:Petroleum; D012987:Soil; D012989:Soil Pollutants; D012822:Silicon Dioxide