title: Two-dimensional crystalline array formation of glucuronide transporter from Escherichia coli by the use of polystyrene beads for detergent removal.
abstract: n-Dodecyl-β-D-maltoside solubilized glucuronide transporter (GusB), the product of gusB gene from Escherichia coli, was treated with Bio-Beads as an agent for removing the detergent from a micellar solution under suitable combination with dimyristoylphosphatidylcholine. Optimizing conditions led to a two-dimensional crystalline array formation of GusB. The crystalline arrays appear to have a hexagonal lattice with layer group P6, the unit cell dimensions of a = b = 13.8 nm and γ = 120°. Each stain-protruding periodic unit showed approximately 11.8 ± 0.3 nm in a diameter in the inverse Fourier-filtered image to have formed with pentameric GusB (5 × 49.7 kDa).
authors.affiliation: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba Central-6 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. ishii@ni.aist.go.jp
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Ishii
mesh_terms: D003460:Crystallization; D003902:Detergents / Q000737:chemistry*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000737:chemistry* / Q000378:metabolism; D008823:Micelles; D011137:Polystyrenes / Q000737:chemistry*; D012995:Solubility
chemical_list: D003902:Detergents; D029968:Escherichia coli Proteins; C499989:GusB protein, E coli; D026901:Membrane Transport Proteins; D008823:Micelles; D011137:Polystyrenes; D004134:Dimyristoylphosphatidylcholine