title: Visible-to-near IR quantum dot-based hypermulticolor high-content screening of herbal medicines for the efficacy monitoring of hair growth promotion and hair loss inhibition.
abstract: There is a growing interest in alopecia prevention strategies, as the number of alopecia patients is increasing. We examine the efficacy of herbal medicine for hair growth promotion/hair loss inhibition in two cell lines via Western blot and high-content screening (HCS). Nine herbal extracts were obtained from three different herbal medicine mixtures using 3 different extraction methods. Five target proteins-IGF-1 (insulin-like growth factor-1), TGF-β2 (transforming growth factor-β2), VEGF (vascular endothelial growth factor), DKK-1 (Dickkopf-1), and Wnt5α-were observed for the assessment of hair growth promotion/hair loss inhibition efficacy. The efficacies of nine extracts were compared with minoxidil as control. Efficacy was defined as a rise in the expression levels of IGF-1, VEGF, and Wnt5α but a decrease in DKK-1 and TGF-β2. Intracellular concurrent imaging of these proteins was successfully achieved using HCS, employing visible-to-near infrared probing based on quantum-antibody conjugates and hypermulticolor imaging.
authors.affiliation: College of Pharmacy, Seoul National University, Seoul, South Korea.
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Kim
authors.forename: Chaeyun
authors.initials: C
authors.lastname: Lim
authors.forename: Jun Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Kyung Ran
authors.initials: KR
authors.lastname: Im
authors.forename: Kyung-Sup
authors.initials: KS
authors.lastname: Yoon
authors.forename: Joon Myong
authors.initials: JM
authors.lastname: Song
mesh_terms: D000505:Alopecia / Q000188:drug therapy* / Q000517:prevention & control*; D015153:Blotting, Western; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D005434:Flow Cytometry; D006197:Hair / Q000187:drug effects / Q000254:growth & development*; D018859:Hair Follicle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008914:Minoxidil / Q000494:pharmacology; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D010946:Plants, Medicinal / Q000737:chemistry*; D011506:Proteins / Q000378:metabolism; D045663:Quantum Dots; D019265:Spectroscopy, Near-Infrared; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
chemical_list: D010936:Plant Extracts; D011506:Proteins; D008914:Minoxidil