title: Physiological and enzymatic comparison between Pichia stipitis and recombinant Saccharomyces cerevisiae on xylose fermentation.
abstract: In order to better understand the differences in xylose metabolism between natural xylose-utilizing Pichia stipitis and metabolically engineered Saccharomyces cerevisiae, we constructed a series of recombinant S. cerevisiae strains with different xylose reductase/xylitol dehydrogenase/xylulokinase activity ratios by integrating xylitol dehydrogenase gene (XYL2) into the chromosome with variable copies and heterogeneously expressing xylose reductase gene (XYL1) and endogenous xylulokinase gene (XKS1). The strain with the highest specific xylose uptake rate and ethanol productivity on pure xylose fermentation was selected to compare to P. stipitis under oxygen-limited condition. Physiological and enzymatic comparison showed that they have different patterns of xylose metabolism and NADPH generation.
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China. guocy74@gmail.com
authors.forename: Changying
authors.initials: C
authors.lastname: Guo
authors.forename: Ning
authors.initials: N
authors.lastname: Jiang
mesh_terms: D000449:Aldehyde Reductase / Q000235:genetics / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D050542:D-Xylulose Reductase / Q000235:genetics / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D009249:NADP / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism; D010843:Pichia / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D014993:Xylitol / Q000378:metabolism; D014994:Xylose / Q000378:metabolism*
chemical_list: D003470:Culture Media; D000431:Ethanol; D009249:NADP; D014994:Xylose; D000449:Aldehyde Reductase; D050542:D-Xylulose Reductase; D017853:Phosphotransferases (Alcohol Group Acceptor); C032700:xylulokinase; D010100:Oxygen; D014993:Xylitol