title: Effect of ASCs on the graft survival rates of fat particles in rabbits.
abstract: This study was conducted to explore the effect of adipose-derived stem cells (ASCs) on the graft survival rates of fat particles in rabbits. Six domestic rabbits were used for a 3-month study; 1.4 grams of fat tissues were harvested from the bilateral inguinal regions of each animal. They were cut into granules and divided into three parts (A = 0.4 g, B = 0.4 g, and C = 0.6 g). Part A was centrifuged after 0.075% collagenase digestion for isolation of the stromal vascular fraction (SVF). About 0.2 grams of SVF containing ASCs was obtained, and then incorporated with part B to create a treated group, whereas part C was treated as a control group. The tissues in both groups were randomly transplanted into a subcutaneous space that had been created on each side of the dorsal midline of the rabbit. The grafts were taken out after 3 months for calculation of the survival rates. The graft survival rate in the treated group was 23.56 ± 2.49%, while that in the control group was 11.06 ± 2.10%. The graft survival rate in the treated group increased significantly, compared with in the control group (p < 0.01). Improved transplantation effects may be obtained by implanting the fat particles mixed with ASCs. It is suggested that this approach has the potential for becoming a new method of fat graft in clinical practice.
authors.affiliation: The Affiliated Hospital of Hangzhou Nomal University, Hangzhou, Zhejiang, PR China.
authors.forename: Xiaosheng
authors.initials: X
authors.lastname: He
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Zhong
authors.forename: Youdi
authors.initials: Y
authors.lastname: Ni
authors.forename: Maolin
authors.initials: M
authors.lastname: Liu
authors.forename: Suhang
authors.initials: S
authors.lastname: Liu
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Lan
mesh_terms: D017667:Adipocytes / Q000637:transplantation*; D050156:Adipogenesis / Q000502:physiology*; D000273:Adipose Tissue / Q000637:transplantation*; D000818:Animals; D001707:Biopsy, Needle; D004195:Disease Models, Animal; D005434:Flow Cytometry; D006084:Graft Rejection; D006085:Graft Survival; D007150:Immunohistochemistry; D011817:Rabbits; D011897:Random Allocation; D012016:Reference Values; D018570:Risk Assessment; D033581:Stem Cell Transplantation / Q000009:adverse effects / Q000379:methods*; D014182:Transplantation, Autologous; D016896:Treatment Outcome