title: MiRGator v3.0: a microRNA portal for deep sequencing, expression profiling and mRNA targeting.
abstract: Biogenesis and molecular function are two key subjects in the field of microRNA (miRNA) research. Deep sequencing has become the principal technique in cataloging of miRNA repertoire and generating expression profiles in an unbiased manner. Here, we describe the miRGator v3.0 update (http://mirgator.kobic.re.kr) that compiled the deep sequencing miRNA data available in public and implemented several novel tools to facilitate exploration of massive data. The miR-seq browser supports users to examine short read alignment with the secondary structure and read count information available in concurrent windows. Features such as sequence editing, sorting, ordering, import and export of user data would be of great utility for studying iso-miRs, miRNA editing and modifications. miRNA-target relation is essential for understanding miRNA function. Coexpression analysis of miRNA and target mRNAs, based on miRNA-seq and RNA-seq data from the same sample, is visualized in the heat-map and network views where users can investigate the inverse correlation of gene expression and target relations, compiled from various databases of predicted and validated targets. By keeping datasets and analytic tools up-to-date, miRGator should continue to serve as an integrated resource for biogenesis and functional investigation of miRNAs.
authors.affiliation: Ewha Research Center for Systems Biology (ERCSB), Ewha Womans University, Seoul 120-750, Korea.
authors.forename: Sooyoung
authors.initials: S
authors.lastname: Cho
authors.forename: Insu
authors.initials: I
authors.lastname: Jang
authors.forename: Yukyung
authors.initials: Y
authors.lastname: Jun
authors.forename: Suhyeon
authors.initials: S
authors.lastname: Yoon
authors.forename: Minjeong
authors.initials: M
authors.lastname: Ko
authors.forename: Yeajee
authors.initials: Y
authors.lastname: Kwon
authors.forename: Ikjung
authors.initials: I
authors.lastname: Choi
authors.forename: Hyeshik
authors.initials: H
authors.lastname: Chang
authors.forename: Daeun
authors.initials: D
authors.lastname: Ryu
authors.forename: Byungwook
authors.initials: B
authors.lastname: Lee
authors.forename: V Narry
authors.initials: VN
authors.lastname: Kim
authors.forename: Wankyu
authors.initials: W
authors.lastname: Kim
authors.forename: Sanghyuk
authors.initials: S
authors.lastname: Lee
mesh_terms: D030561:Databases, Nucleic Acid; D059014:High-Throughput Nucleotide Sequencing; D020407:Internet; D035683:MicroRNAs / Q000737:chemistry* / Q000378:metabolism*; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger