title: Overexpression of a Chinese cabbage BrERF11 transcription factor enhances disease resistance to Ralstonia solanacearum in tobacco.
abstract: Ethylene-responsive factors (ERFs) play diverse roles in plant growth, developmental processes and stress responses. However, the roles and underlying mechanism of ERFs remain poorly understood, especially in non-model plants. In this study, a full length cDNA of ERF gene was isolated from the cDNA library of Chinese cabbage. According to sequence alignment, we found a highly conservative AP2/ERF domain, two nuclear localization signals, and an ERF-associated Amphiphilic Repression (EAR) motif in its C-terminal region. It belonged to VIIIa group ERFs sharing the highest sequence identity with AtERF11 in all of the ERFs in Arabidopsis and designated BrERF11. BrERF11-green fluorescence protein (GFP) transient expressed in onion epidermis cells localized to the nucleus. The transcript levels of BrERF11 were induced by exogenous salicylic acid (SA), methyl jasmonate (MeJA), ethephon (ETH), and hydrogen peroxide (H(2)O(2)). Constitutive expression of BrERF11 enhanced tolerance to Ralstonia solanacearum infection in transgenic tobacco plants, which was coupled with hypersensitive response (HR), burst of H(2)O(2) and upregulation of defense-related genes including HR marker genes, SA-, JA-dependent pathogen-related genes and ET biosynthesis associated genes and downregulation of CAT1, suggesting BrERF11 may participate in pathogen-associated molecular pattern (PAMP)- and effector-triggered immunity (PTI and ETI) mediated by SA-, JA- and ET-dependent signaling mechanisms.
authors.affiliation: College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lai
authors.forename: Fengfeng
authors.initials: F
authors.lastname: Dang
authors.forename: Jing
authors.initials: J
authors.lastname: Lin
authors.forename: Lu
authors.initials: L
authors.lastname: Yu
authors.forename: Youliang
authors.initials: Y
authors.lastname: Shi
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Xiao
authors.forename: Mukun
authors.initials: M
authors.lastname: Huang
authors.forename: Jinhui
authors.initials: J
authors.lastname: Lin
authors.forename: Chengcong
authors.initials: C
authors.lastname: Chen
authors.forename: Aihua
authors.initials: A
authors.lastname: Qi
authors.forename: Zhiqin
authors.initials: Z
authors.lastname: Liu
authors.forename: Deyi
authors.initials: D
authors.lastname: Guan
authors.forename: Shaoliang
authors.initials: S
authors.lastname: Mou
authors.forename: Ailian
authors.initials: A
authors.lastname: Qiu
authors.forename: Shuilin
authors.initials: S
authors.lastname: He
mesh_terms: D001937:Brassica / Q000235:genetics*; D060467:Disease Resistance; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D010935:Plant Diseases; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D043368:Ralstonia solanacearum; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors