title: Microsatellite loci for tucumã of Amazonas (Astrocaryum aculeatum) and amplification in other Arecaceae.
abstract: OBJECTIVE
  Microsatellite loci were developed for tucumã of Amazonas (Astrocaryum aculeatum), and cross-species amplification was performed in six other Arecaceae, to investigate genetic diversity and population structure and to provide support for natural populations management. •
  
  
  RESULTS
  Fourteen microsatellite loci were isolated from a microsatellite-enriched genomic library and used to characterize two wild populations of tucumã of Amazonas (Manaus and Manicoré cities). The investigated loci displayed high polymorphism for both A. aculeatum populations, with a mean observed heterozygosity of 0.498. Amplification rates ranging from 50% to 93% were found for four Astrocaryum species and two additional species of Arecaceae. •
  
  
  CONCLUSIONS
  The information derived from the microsatellite markers developed here provides significant gains in conserved allelic richness and supports the implementation of several molecular breeding strategies for the Amazonian tucumã.
authors.affiliation: Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, CP 83, CEP 13400-970, Piracicaba, São Paulo, Brazil.
authors.forename: Santiago L Ferreyra
authors.initials: SL
authors.lastname: Ramos
authors.forename: Jeferson L Vasconcelos
authors.initials: JL
authors.lastname: de Macêdo
authors.forename: Maria T Gomes
authors.initials: MT
authors.lastname: Lopes
authors.forename: Jacqueline S
authors.initials: JS
authors.lastname: Batista
authors.forename: Kyara M
authors.initials: KM
authors.lastname: Formiga
authors.forename: Perla Pimentel
authors.initials: PP
authors.lastname: da Silva
authors.forename: Antonio C
authors.initials: AC
authors.lastname: Saulo-Machado
authors.forename: Elizabeth Ann
authors.initials: EA
authors.lastname: Veasey
mesh_terms: D028023:Arecaceae / Q000235:genetics*; D001938:Brazil; D017931:DNA Primers / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018744:DNA, Plant