title: [Cloning of Eleutherococcus senticosus calmodulin gene and effect of endophytic fungus on expression amount of gene].
abstract: OBJECTIVE
  To clone calmodulin (CaM) gene in Eleutherococcus senticosus, and study the effect of endophytic fungi on expression amount of CaM gene.
  
  
  METHODS
  The CaM full length cDNA sequence was cloned by rapid amplification of cDNA ends (RACE). The gene was analyzed and corresponding structure and functions were predicted by the bioinformatics methods. The expression amount of CaM gene affected of endophytic fungus P116-1a, P116-1b, P1094 and P312-1 was detected by RT-PCR.
  
  
  RESULTS
  The full length of CaM cDNA was 856 bp containing an ORF of 450 bp that encoded a protein of 149 amino acids. The homologous of predicted protein was almost 100% with plants like Panax ginseng and Daucus carota. RT-PCR results showed that endophytic fungus improved CaM expression amount significantly (P<0.05). The highest expression amount of CaM occurred 90 d after reinoculated with endophytic fungi P1094, up to 2.96 times of the control.
  
  
  CONCLUSIONS
  The CaM gene of E. senticosus was successfully cloned for the first time. The results demonstrated that endophytic fungus of E. senticosus improved CaM expression amount significantly.
authors.affiliation: College of Life Science, Hebei United University, Tangshan 063000, China. xingzhaobin@yahoo.com.cn
authors.forename: Zhaobin
authors.initials: Z
authors.lastname: Xing
authors.forename: Yuehong
authors.initials: Y
authors.lastname: Long
authors.forename: Baocai
authors.initials: B
authors.lastname: Li
authors.forename: Jinli
authors.initials: J
authors.lastname: Zhu
authors.forename: Shan
authors.initials: S
authors.lastname: He
mesh_terms: D002147:Calmodulin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D028001:Eleutherococcus / Q000145:classification / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*; D060026:Endophytes / Q000502:physiology*; D005658:Fungi / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D002147:Calmodulin; D010940:Plant Proteins