title: Method of 2,3-butanediol production from glycerol and acid-pretreated rice straw hydrolysate by newly isolated strains: pre-evaluation as an integrated biorefinery process.
abstract: The present study validated a bioconversion technology for the production of 2,3-butanediol (2,3-BD) using sugars, glycerol and lignocellulosic material by three newly isolated strains-two Klebsiella sp. and one Serratia sp. One Klebsiella sp. afforded a high diol production yield (0.45 g/g) using the less common sugar arabinose and Serratia sp. was used for the first time to convert glycerol to 2,3-BD and afforded a yield of 0.43 g/g. Furthermore, acid-pretreated rice straw hydrolysate was used to determine the feasibility of its conversion to 2,3-BD. Both cellulose and hemicellulose hydrolysate were successfully fermented to 2,3-BD and acetoin by the isolates with yields for the diol between 0.39 and 0.44 g/g (equivalent to 78-88% of the maximum yield). These results demonstrate that 2,3-butanediol can be considered as the main product or a value-added byproduct of biofuel production and then potentially improve the economy of lignocellulosic biorefinery.
authors.affiliation: Cellulosic Ethanol Project, Institute of Nuclear Energy Research, Taoyuan County 32546, Taiwan, ROC.
authors.forename: Chiung-Fang
authors.initials: CF
authors.lastname: Huang
authors.forename: Yi-Feng
authors.initials: YF
authors.lastname: Jiang
authors.forename: Gia-Luen
authors.initials: GL
authors.lastname: Guo
authors.forename: Wen-Song
authors.initials: WS
authors.lastname: Hwang
mesh_terms: D000143:Acids / Q000494:pharmacology*; D001709:Biotechnology / Q000379:methods*; D002072:Butylene Glycols / Q000378:metabolism*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D005285:Fermentation / Q000187:drug effects; D005990:Glycerol / Q000378:metabolism* / Q000494:pharmacology; D006868:Hydrolysis / Q000187:drug effects; D007700:Kinetics; D007709:Klebsiella / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism; D012275:Oryza / Q000187:drug effects*; D011134:Polysaccharides / Q000378:metabolism; D012705:Serratia / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism; D014866:Waste Products / Q000032:analysis
chemical_list: D000143:Acids; D002072:Butylene Glycols; D011134:Polysaccharides; D014866:Waste Products; C026978:2,3-butylene glycol; C007916:hemicellulose; D005990:Glycerol