title: Aptamer-conjugated multifunctional nanoflowers as a platform for targeting, capture, and detection in laser desorption ionization mass spectrometry.
abstract: Although many different nanomaterials have been tested as substrates for laser desorption and ionization mass spectrometry (LDI-MS), this emerging field still requires more efficient multifuncional nanomaterials for targeting, enrichment, and detection. Here, we report the use of gold manganese oxide (Au@MnO) hybrid nanoflowers as an efficient matrix for LDI-MS. The nanoflowers were also functionalized with two different aptamers to target cancer cells and capture adenosine triphosphate (ATP). These nanoflowers were successfully used for metabolite extraction from cancer cell lysates. Thus, in one system, our multifunctional nanoflowers can (1) act as an ionization substrate for mass spectrometry, (2) target cancer cells, and (3) detect and analyze metabolites from cancer cells.
authors.affiliation: Center for Research at the Bio/Nano Interface, Department of Chemistry and Shands Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, Florida 32611, USA.
authors.forename: Ismail
authors.initials: I
authors.lastname: Ocsoy
authors.forename: Basri
authors.initials: B
authors.lastname: Gulbakan
authors.forename: Mohammed Ibrahim
authors.initials: MI
authors.lastname: Shukoor
authors.forename: Xiangling
authors.initials: X
authors.lastname: Xiong
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.forename: David H
authors.initials: DH
authors.lastname: Powell
authors.forename: Weihong
authors.initials: W
authors.lastname: Tan
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D052157:Aptamers, Nucleotide / Q000235:genetics / Q000493:pharmacokinetics; D045744:Cell Line, Tumor; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*; D053769:Nanocapsules / Q000737:chemistry*; D009374:Neoplasms, Experimental / Q000235:genetics / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide; D053769:Nanocapsules; D000255:Adenosine Triphosphate