title: Optimized mixture of hops rho iso-alpha acids-rich extract and acacia proanthocyanidins-rich extract reduces insulin resistance in 3T3-L1 adipocytes and improves glucose and insulin control in db/db mice.
abstract: Rho iso-alpha acids-rich extract (RIAA) from Humulus lupulus (hops) and proanthocyanidins-rich extracts (PAC) from Acacia nilotica exert anti-inflammatory and anti-diabetic activity in vitro and in vivo. We hypothesized that a combination of these two extracts would exert enhanced effects in vitro on inflammatory markers and insulin signaling, and on nonfasting glucose and insulin in db/db mice. Over 49 tested combinations, RIAA:PAC at 5:1 (6.25 µg/mL) exhibited the greatest reductions in TNFα-stimulated lipolysis and IL-6 release in 3T3-L1 adipocytes, comparable to 5 µg/mL troglitazone. Pretreatment of 3T3-L1 adipocytes with this combination (5 µg/mL) also led to a 3-fold increase in insulin-stimulated glucose uptake that was comparable to 5 µg/mL pioglitazone or 901 µg/mL aspirin. Finally, db/db mice fed with RIAA:PAC at 5:1 (100 mg/kg) for 7 days resulted in 22% decrease in nonfasting glucose and 19% decrease in insulin that was comparable to 0.5 mg/kg rosiglitazone and better than 100 mg/kg metformin. RIAA:PAC mixture may have the potential to be an alternative when conventional therapy is undesirable or ineffective, and future research exploring its long-term clinical application is warranted.
authors.affiliation: MetaProteomics LLC, 9770 44  Ave. NW, Ste 100, Gig Harbor, WA 98332, USA.
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Tripp
authors.forename: Gary
authors.initials: G
authors.lastname: Darland
authors.forename: Veera Reddy
authors.initials: VR
authors.lastname: Konda
authors.forename: Linda M
authors.initials: LM
authors.lastname: Pacioretty
authors.forename: Jyh-Lurn
authors.initials: JL
authors.lastname: Chang
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Bland
authors.forename: John G
authors.initials: JG
authors.lastname: Babish