title: Power generation from a hybrid biological fuel cell in seawater.
abstract: A hybrid biological fuel cell (HBFC) comprised of a microbial anode for lactate oxidation and an enzymatic cathode for oxygen reduction was constructed and then tested in a marine environment. Shewanella oneidensis DSP-10 was cultivated in laboratory medium and then fixed on a carbon felt electrode via a silica sol-gel process in order to catalyze anodic fuel cell processes. The cathode electrocatalyst was composed of bilirubin oxidase, fixed to a carbon nanotube electrode using a heterobifunctional cross linker, and then stabilized with a silica sol-gel coating. The anode and cathode half-cells provided operating potentials of -0.44 and 0.48 V, respectively (vs. Ag/AgCl). The HBFC maintained a reproducible open circuit voltage >0.7 V for 9 d in laboratory settings and sustained electrocatalytic activity for >24h in open environment tests.
authors.affiliation: Microbiology and Applied Biochemistry Laboratory, Airbase Technologies Division, US Air Force Research Laboratory, Tyndall Air Force Base, FL 32403, United States.
authors.forename: Guinevere
authors.initials: G
authors.lastname: Strack
authors.forename: Heather R
authors.initials: HR
authors.lastname: Luckarift
authors.forename: Susan R
authors.initials: SR
authors.lastname: Sizemore
authors.forename: Robert K
authors.initials: RK
authors.lastname: Nichols
authors.forename: Karen E
authors.initials: KE
authors.lastname: Farrington
authors.forename: Peter K
authors.initials: PK
authors.lastname: Wu
authors.forename: Plamen
authors.initials: P
authors.lastname: Atanassov
authors.forename: Justin C
authors.initials: JC
authors.lastname: Biffinger
authors.forename: Glenn R
authors.initials: GR
authors.lastname: Johnson
mesh_terms: D001674:Bioelectric Energy Sources / Q000382:microbiology*; D004566:Electrodes; D004735:Energy Transfer; D012623:Seawater / Q000382:microbiology*; D020592:Shewanella / Q000145:classification / Q000502:physiology*; D013045:Species Specificity