title: Combination of anti-biofouling and ion-interaction by click chemistry for endotoxin selective removal from protein solution.
abstract: A successful combination of anti-biofouling and ion-interaction for endotoxin (ET) selective removal from protein solutions is realized via the immobilization of two ordinary polymers, PEG and PLL, which have protein resistance and endotoxin adsorption properties, respectively, together onto azide-functional polystyrene (PS-N3 ) microspheres (MSs) using a surface click reaction. Toxic copper ions residu is successfully minimized by the addition of 2,2'-bipyridine (BiPy).
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.
authors.forename: Jinshan
authors.initials: J
authors.lastname: Guo
authors.forename: Fanbo
authors.initials: F
authors.lastname: Meng
authors.forename: Xiabin
authors.initials: X
authors.lastname: Jing
authors.forename: Yubin
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000042:Absorption; D057930:Click Chemistry / Q000295:instrumentation / Q000379:methods; D045424:Complex Mixtures / Q000302:isolation & purification; D004731:Endotoxins / Q000737:chemistry / Q000302:isolation & purification*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007477:Ions; D019344:Lactic Acid / Q000737:chemistry*; D011091:Polyesters; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D012996:Solutions; D014462:Ultrafiltration / Q000295:instrumentation* / Q000379:methods*
chemical_list: D045424:Complex Mixtures; D004731:Endotoxins; D007477:Ions; D011091:Polyesters; D011108:Polymers; D011506:Proteins; D012996:Solutions; D019344:Lactic Acid; D011092:Polyethylene Glycols; C033616:poly(lactide)