title: The influence of rhein 8-O-β-D-glucopyranoside on the purgative action of sennoside A from rhubarb in mice.
abstract: Rhubarb is one of the most well-known herbal medicines that constitute daiokanzoto (DKT), which is clinically effective for constipation. Sennoside A is transformed into an active metabolite, rheinanthrone, by intestinal bacteria. Sennoside A in rhubarb showed significantly accelerated metabolic activity in intestinal bacteria in comparison with sennoside A alone. In this study, we investigated the influence of rhubarb constituents on the metabolism and purgative activity of sennoside A. The 20% MeOH-eluted fraction separated by MCI-gel CHP-20P column chromatography from the water extract of rhubarb showed sennoside A metabolic activity similar to that of rhubarb extract. The 20% MeOH elute was further purified and rhein 8-O-β-D-glucopyranoside (RG) was isolated. The metabolic activity of sennoside A was significantly accelerated by increasing the level of RG. Moreover, rhein, emodin and aloe-emodin also accelerated sennoside A metabolism. The purgative activity of sennoside A was significantly accelerated when RG or rhein was concomitantly given with sennoside A in a dose-dependent manner. These results suggest that anthraquinones contribute to the purgative action of sennoside A in rhubarb. Therefore, it is assumed that the influence of anthraquinones on the fate of rheinanthrone transformed from sennoside A may promote the purgative action of sennoside A.
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, 1 Sanzo, Gakuen-cho, Fukuyama, Hiroshima 729–0292, Japan. takayama@fupharm.fukuyama-u.ac.jp
authors.forename: Kento
authors.initials: K
authors.lastname: Takayama
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsutsumi
authors.forename: Takashi
authors.initials: T
authors.lastname: Ishizu
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Okamura
mesh_terms: D000818:Animals; D000873:Anthracenes / Q000378:metabolism / Q000494:pharmacology*; D000880:Anthraquinones / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D001419:Bacteria / Q000378:metabolism; D002400:Cathartics / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D003248:Constipation / Q000188:drug therapy; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D004642:Emodin / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000382:microbiology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009005:Monosaccharides / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D008517:Phytotherapy; D012250:Rheum / Q000737:chemistry*; D012676:Senna Extract / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D000081226:Sennosides; D000095322:Anthrones
chemical_list: D000873:Anthracenes; D000880:Anthraquinones; D002400:Cathartics; D004365:Drugs, Chinese Herbal; D004642:Emodin; D009005:Monosaccharides; D012676:Senna Extract; D000081226:Sennosides; C581104:rhein 8-O-beta-D-glucopyranoside; C055876:rhein-9-anthrone; D000095322:Anthrones; C518327:aloe emodin; C020491:rhein