title: Detection of ricin intoxication in mice using serum peptide profiling by MALDI-TOF/MS.
abstract: Ricin toxin has been regarded as one of the most potent poisons in the plant kingdom, and there is no effective therapeutic countermeasure or licensed vaccine against it. Consequently, early detection of ricin intoxication is necessary. In this study, we took mice as test subjects, and used the technique of Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/MS) and ClinProt™ microparticle beads to set up an effective detection model with an accuracy of almost 100%. Eighty-two peaks in the mass range 1000-10,000 m/z were detected by ClinProTools software, and five different peaks with m/z of 4982.49, 1333.25, 1537.86, 4285.05 and 2738.88 had the greatest contribution to the accuracy and sensitivity of this model. They may therefore provide biomarkers for ricin intoxication.
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, 666 West Liuying Road, Changchun, Jilin 130122, China. qianjunqj_11@126.com.
authors.forename: Siyan
authors.initials: S
authors.lastname: Zhao
authors.forename: Wen-Sen
authors.initials: WS
authors.lastname: Liu
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.forename: Jiping
authors.initials: J
authors.lastname: Li
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Sun
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Hou
authors.forename: Jia-Yu
authors.initials: JY
authors.lastname: Wan
authors.forename: Zhongyi
authors.initials: Z
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
authors.forename: Linna
authors.initials: L
authors.lastname: Liu
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D005260:Female; D007274:Injections, Intraperitoneal; D008280:Magnetics; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000097:blood*; D012276:Ricin / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
chemical_list: D015415:Biomarkers; D010455:Peptides; D012276:Ricin