title: Population structure and circulating genotypes of drug-sensitive and drug-resistant Mycobacterium tuberculosis clinical isolates in São Paulo state, Brazil.
abstract: São Paulo is the most populous Brazilian state and reports the largest number of tuberculosis cases in the country annually (over 18,500). This study included 193 isolates obtained during the 2nd Nationwide Survey on Mycobacterium tuberculosis Drug Resistance that was conducted in São Paulo state and 547 isolates from a laboratory based study of drug resistance that were analyzed by the Mycobacteria Reference Laboratory at the Institute Adolfo Lutz. Both studies were conducted from 2006 to 2008 and sought to determine the genetic diversity and pattern of drug resistance of M. tuberculosis isolates (MTC) circulating in São Paulo. The patterns obtained from the spoligotyping analysis demonstrated that 51/740 (6.9%) of the isolates corresponded to orphan patterns and that 689 (93.1%) of the isolates distributed into 144 shared types, including 119 that matched a preexisting shared type in the SITVIT2 database and 25 that were new isolates. A total of 77/144 patterns corresponded to unique isolates, while the remaining 67 corresponded to clustered patterns (n=612 isolates clustered into groups of 2-84 isolates each). The evolutionarily ancient PGG1 lineages (Beijing, CAS1-DEL, EAI3-IND, and PINI2) were rarely detected in São Paulo and comprised only 13/740, or 1.76%, of the total isolates; all of the remaining 727/740, or 98.24%, of the MTC isolates from São Paulo state were from the recent PGG2/3 evolutionary isolates belonging to the LAM, T, S, X, and Haarlem lineages, i.e., the Euro-American group. This study provides the first overview of circulating genotypes of M. tuberculosis in São Paulo state and demonstrates that the clustered shared types containing seven or more M. tuberculosis isolates that are spread in São Paulo state included both resistant and susceptible isolates.
authors.affiliation: Núcleo de Tuberculose e Micobacterioses, Centro de Bacteriologia, Instituto Adolfo Lutz, São Paulo, Brazil. martinsesouza@uol.com.br
authors.forename: Maria Conceição
authors.initials: MC
authors.lastname: Martins
authors.forename: Carmen M Saraiva
authors.initials: CM
authors.lastname: Giampaglia
authors.forename: Rosângela S
authors.initials: RS
authors.lastname: Oliveira
authors.forename: Vera
authors.initials: V
authors.lastname: Simonsen
authors.forename: Fábio Oliveira
authors.initials: FO
authors.lastname: Latrilha
authors.forename: Letícia Lisboa
authors.initials: LL
authors.lastname: Moniz
authors.forename: David
authors.initials: D
authors.lastname: Couvin
authors.forename: Nalin
authors.initials: N
authors.lastname: Rastogi
authors.forename: Lucilaine
authors.initials: L
authors.lastname: Ferrazoli
mesh_terms: D001938:Brazil; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification; D014376:Tuberculosis / Q000453:epidemiology*; D018088:Tuberculosis, Multidrug-Resistant / Q000235:genetics*