title: Hydrogel limits stem cell dispersal in the deaf cochlea: implications for cochlear implants.
abstract: Auditory neurons provide the critical link between a cochlear implant and the brain in deaf individuals, therefore their preservation and/or regeneration is important for optimal performance of this neural prosthesis. In cases where auditory neurons are significantly depleted, stem cells (SCs) may be used to replace the lost population of neurons, thereby re-establishing the critical link between the periphery (implant) and the brain. For such a therapy to be therapeutically viable, SCs must be differentiated into neurons, retained at their delivery site and damage caused to the residual auditory neurons minimized. Here we describe the transplantation of SC-derived neurons into the deaf cochlea, using a peptide hydrogel to limit their dispersal. The described approach illustrates that SCs can be delivered to and are retained within the basal turn of the cochlea, without a significant loss of endogenous auditory neurons. In addition, the tissue response elicited from this surgical approach was restricted to the surgical site and did not extend beyond the cochlear basal turn. Overall, this approach illustrates the feasibility of targeted cell delivery into the mammalian cochlea using hydrogel, which may be useful for future cell-based transplantation strategies, for combined treatment with a cochlear implant to restore function.
authors.affiliation: Department of Otolaryngology, University of Melbourne, Australia. b.nayagam@unimelb.edu.au
authors.forename: Bryony A
authors.initials: BA
authors.lastname: Nayagam
authors.forename: Steven S
authors.initials: SS
authors.lastname: Backhouse
authors.forename: Cengiz
authors.initials: C
authors.lastname: Cimenkaya
authors.forename: Robert K
authors.initials: RK
authors.lastname: Shepherd
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D003051:Cochlea / Q000166:cytology / Q000502:physiology*; D003054:Cochlear Implants; D003638:Deafness / Q000628:therapy*; D005260:Female; D006168:Guinea Pigs; D020100:Hydrogels; D007150:Immunohistochemistry; D007249:Inflammation / Q000473:pathology; D008297:Male; D010455:Peptides / Q000008:administration & dosage / Q000494:pharmacology; D033581:Stem Cell Transplantation / Q000379:methods*
chemical_list: D020100:Hydrogels; D010455:Peptides