title: Novel polymeric inhibitors of HCoV-NL63.
abstract: The human coronavirus NL63 is generally classified as a common cold pathogen, though the infection may also result in severe lower respiratory tract diseases, especially in children, patients with underlying disease, and elderly. It has been previously shown that HCoV-NL63 is also one of the most important causes of croup in children. In the current manuscript we developed a set of polymer-based compounds showing prominent anticoronaviral activity. Polymers have been recently considered as promising alternatives to small molecule inhibitors, due to their intrinsic antimicrobial properties and ability to serve as matrices for antimicrobial compounds. Most of the antimicrobial polymers show antibacterial properties, while those with antiviral activity are much less frequent. A cationically modified chitosan derivative, N-(2-hydroxypropyl)-3-trimethylammonium chitosan chloride (HTCC), and hydrophobically-modified HTCC were shown to be potent inhibitors of HCoV-NL63 replication. Furthermore, both compounds showed prominent activity against murine hepatitis virus, suggesting broader anticoronaviral activity.
authors.affiliation: Microbiology Department, Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Milewska
authors.forename: Justyna
authors.initials: J
authors.lastname: Ciejka
authors.forename: Kamil
authors.initials: K
authors.lastname: Kaminski
authors.forename: Anna
authors.initials: A
authors.lastname: Karewicz
authors.forename: Dorota
authors.initials: D
authors.lastname: Bielska
authors.forename: Slawomir
authors.initials: S
authors.lastname: Zeglen
authors.forename: Wojciech
authors.initials: W
authors.lastname: Karolak
authors.forename: Maria
authors.initials: M
authors.lastname: Nowakowska
authors.forename: Jan
authors.initials: J
authors.lastname: Potempa
authors.forename: Berend Jan
authors.initials: BJ
authors.lastname: Bosch
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Pyrc
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Szczubialka
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D002412:Cations / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D058957:Coronavirus NL63, Human / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D008253:Macaca mulatta; D006517:Murine hepatitis virus / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D002412:Cations; D048271:Chitosan