title: Spray- and laser-assisted biomaterial processing for fast and efficient autologous cell-plus-matrix tissue engineering.
abstract: At present, intensive investigation aims at the creation of optimal valvular prostheses. We introduced and tested the applicability and functionality of two advanced cell-plus-matrix seeding technologies, spray-assisted bioprocessing (SaBP) and laser-assisted bioprocessing (LaBP), for autologous tissue engineering (TE) of bioresorbable artificial grafts. For SaBP, human mesenchymal stem cells (HMSCs), umbilical cord vein endothelial cells (HUVECs) and fibrin were simultaneously spray-administered on poly(ε-caprolactone) (PCL) substrates. For LaBP, HUVECs and HMSCs were separately laser-printed in stripes, followed by fibrin sealing. Three-leaflet valves were manufactured following TE of electrospun PCL tissue equivalents. Grafts were monitored in vitro under static and dynamic conditions in bioreactors. SaBP and LaBP resulted in TE of grafts with homogeneous cell distribution and accurate cell pattern, respectively. The engineered valves demonstrated immediate sufficient performance, complete cell coating, proliferation, engraftment, HUVEC-mediated invasion, HMSC differentiation and extracellular matrix deposition. SaBP revealed higher efficiency, with at least 12-fold shorter processing time than the applied LaBP set-up. LaBP realized coating with higher cell density and minimal cell-scaffold distance. Fibrin and PCL stability remain issues for improvement. The introduced TE technologies resulted in complete valvular cell-plus-matrix coating, excellent engraftment and HMSCs differentiation. SaBP might have potential for intraoperative table-side TE considering the procedural duration and ease of implementation. LaBP might accelerate engraftment with precise patterns.
authors.affiliation: Reference and Translation Centre for Cardiac Stem Cell Therapy, University of Rostock, Germany.|Department of Cardiac Surgery, Medical Faculty, University of Rostock, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Klopsch
authors.affiliation: Reference and Translation Centre for Cardiac Stem Cell Therapy, University of Rostock, Germany.|Department of Cardiac Surgery, Medical Faculty, University of Rostock, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Gäbel
authors.affiliation: Reference and Translation Centre for Cardiac Stem Cell Therapy, University of Rostock, Germany.|Department of Cardiac Surgery, Medical Faculty, University of Rostock, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Kaminski
authors.affiliation: Reference and Translation Centre for Cardiac Stem Cell Therapy, University of Rostock, Germany.|Department of Cardiac Surgery, Medical Faculty, University of Rostock, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Mark
authors.affiliation: Reference and Translation Centre for Cardiac Stem Cell Therapy, University of Rostock, Germany.|Department of Cardiac Surgery, Medical Faculty, University of Rostock, Germany.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Reference and Translation Centre for Cardiac Stem Cell Therapy, University of Rostock, Germany.|Department of Cardiac Surgery, Medical Faculty, University of Rostock, Germany.
authors.forename: Anita
authors.initials: A
authors.lastname: Toelk
authors.affiliation: Reference and Translation Centre for Cardiac Stem Cell Therapy, University of Rostock, Germany.|Department of Cardiac Surgery, Medical Faculty, University of Rostock, Germany.
authors.forename: Evgenya
authors.initials: E
authors.lastname: Delyagina
authors.affiliation: Reference and Translation Centre for Cardiac Stem Cell Therapy, University of Rostock, Germany.|Department of Cardiac Surgery, Medical Faculty, University of Rostock, Germany.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Kleiner
authors.affiliation: Laser Zentrum Hannover, Germany.
authors.forename: Lothar
authors.initials: L
authors.lastname: Koch
authors.affiliation: Laser Zentrum Hannover, Germany.
authors.forename: Boris
authors.initials: B
authors.lastname: Chichkov
authors.affiliation: Department of Tissue Engineering and Textile Implants, AME-Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Mela
authors.affiliation: Department of Tissue Engineering and Textile Implants, AME-Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Jockenhoevel
authors.affiliation: Reference and Translation Centre for Cardiac Stem Cell Therapy, University of Rostock, Germany.|Department of Cardiac Surgery, Medical Faculty, University of Rostock, Germany.
authors.forename: Nan
authors.initials: N
authors.lastname: Ma
authors.affiliation: Reference and Translation Centre for Cardiac Stem Cell Therapy, University of Rostock, Germany.|Department of Cardiac Surgery, Medical Faculty, University of Rostock, Germany.
authors.forename: Gustav
authors.initials: G
authors.lastname: Steinhoff
mesh_terms: D005109:Extracellular Matrix / Q000737:chemistry*; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D011091:Polyesters / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D011091:Polyesters; C016240:polycaprolactone