title: Crystallization and preliminary X-ray diffraction analysis of the invertase from Saccharomyces cerevisiae.
abstract: Saccharomyces cerevisiae invertase (ScInv) is an enzyme encoded by the SUC2 gene that releases β-fructose from the nonreducing termini of various β-D-fructofuranoside substrates. Its ability to produce 6-kestose by transglycosylation makes this enzyme an interesting research target for applications in industrial biotechnology. The native enzyme, which presents a high degree of oligomerization, was crystallized by vapour-diffusion methods. The crystals belonged to space group P3(1)21, with unit-cell parameters a=268.6, b=268.6, c=224.4 Å. The crystals diffracted to 3.3 Å resolution and gave complete data sets using a synchrotron X-ray source.
authors.affiliation: Departamento de Cristalografía y Biología Estructural, Instituto de Química-Física Rocasolano, CSIC, Serrano 119, 28006 Madrid, Spain.
authors.forename: M Angela
authors.initials: MA
authors.lastname: Sainz-Polo
authors.forename: Alvaro
authors.initials: A
authors.lastname: Lafraya
authors.forename: Aitana
authors.initials: A
authors.lastname: Polo
authors.forename: Julia
authors.initials: J
authors.lastname: Marín-Navarro
authors.forename: Julio
authors.initials: J
authors.lastname: Polaina
authors.forename: Julia
authors.initials: J
authors.lastname: Sanz-Aparicio
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D014312:Trisaccharides / Q000737:chemistry / Q000378:metabolism; D014961:X-Ray Diffraction; D043324:beta-Fructofuranosidase / Q000737:chemistry*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D014312:Trisaccharides; C073432:6-kestose; C468437:SUC2 protein, S cerevisiae; D043324:beta-Fructofuranosidase