title: Increased miRNA-22 expression sensitizes esophageal squamous cell carcinoma to irradiation.
abstract: miRNA-22 was previously reported to be a tumor suppressor. The aim of this study was to explore the expression and function of miRNA-22 in esophageal squamous cell carcinoma (ESCC). Expression of miRNA-22 in 100 ESCC tissues was examined by q-PCR. The correlation between miRNA-22 level and clinicopathological features was analyzed using SPSS16.0 statistical software. Moreover, the effect of miRNA-22 expression on radiosensitivity of ESCC cells was examined. miRNA-22 expression decreased in ESCC tissues, and statistical analyses showed that the expression of miRNA-22 was associated with the stage of clinical classification. No correlation was found between miRNA-22 expression and the overall survival of ESCC patients. However, significant positive correlation was found between miRNA-22 expression and the survival of patients who received radiotherapy (P < 0.05). Increased expression of miRNA-22 sensitized ESCC cells to γ-ray radiation and promoted the apoptosis of ESCC cells induced by γ-ray radiation. Increased expression level of miRNA-22 had effects on Rad51 expression after irradiation. These results demonstrate for the first time that decreased miRNA-22 expression correlates with increased radiotherapy resistance of ESCC, and that this effect is mediated, at least in part, by the Rad51 pathway.
authors.affiliation: Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science, Tianjin, 300192, China. wxc3188@126.com
authors.forename: Xiao-chun
authors.initials: XC
authors.lastname: Wang
authors.forename: Zhu-Bo
authors.initials: ZB
authors.lastname: Zhang
authors.forename: Yue-Ying
authors.initials: YY
authors.lastname: Wang
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Wu
authors.forename: De-Guan
authors.initials: DG
authors.lastname: Li
authors.forename: Ai-Min
authors.initials: AM
authors.lastname: Meng
authors.forename: Fei-Yue
authors.initials: FY
authors.lastname: Fan
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000532:radiotherapy*; D045744:Cell Line, Tumor; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000532:radiotherapy*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000627:therapeutic use*; D011836:Radiation Tolerance / Q000235:genetics*; D014162:Transfection; D016896:Treatment Outcome; D015854:Up-Regulation / Q000235:genetics
chemical_list: C548375:MIRN22 microRNA, human; D035683:MicroRNAs