title: A highly sensitive gold-nanoparticle-based assay for acetylcholinesterase in cerebrospinal fluid of transgenic mice with Alzheimer's disease.
abstract: A highly sensitive, selective, and dual-readout (colorimetric and fluorometric) assay for acetylcholinesterase (AChE) based on Rhodamine B-modified gold nanoparticle is reported. Due to its good sensitivity and selectivity, the assay can be used for monitoring AChE levels in the cerebrospinal fluid of transgenic mice with Alzheimer's disease.
authors.affiliation: CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, 11 Beiyitiao, Zhongguancun, Beijing, 100190, PR China.
authors.forename: Dingbin
authors.initials: D
authors.lastname: Liu
authors.forename: Wenwen
authors.initials: W
authors.lastname: Chen
authors.forename: Yue
authors.initials: Y
authors.lastname: Tian
authors.forename: Sha
authors.initials: S
authors.lastname: He
authors.forename: Wenfu
authors.initials: W
authors.lastname: Zheng
authors.forename: Jiashu
authors.initials: J
authors.lastname: Sun
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Wang
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000110:Acetylcholinesterase / Q000134:cerebrospinal fluid*; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000201:enzymology*; D000818:Animals; D015415:Biomarkers / Q000134:cerebrospinal fluid; D003124:Colorimetry / Q000379:methods*; D006046:Gold; D051379:Mice; D008822:Mice, Transgenic; D053758:Nanoparticles; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; D006046:Gold; D000110:Acetylcholinesterase