title: Antioxidant and hepatoprotective effects of Schisandra chinensis pollen extract on CCl4-induced acute liver damage in mice.
abstract: The aim of the present study was to investigate the antioxidant and hepatotective effects of Schisandra chinensis pollen extract (SCPE) on CCl4-induced acute liver damage in mice. Total phenolic content, total flavonoid content, individual phenolic compounds and antioxidant activities (1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, chelating activity, and reducing power assay) were determined. In vivo study, SCPE (10, 20 and 40g/kg) administered daily orally for 42days prior to CCl4-intoxicated. Our results showed that SCPE had high total phenolic content (53.74±1.21mg GAE/g), total flavonoid content (38.29±0.91mg Rutin/g), quercetin and hesperetin may be the major contributor to strong antioxidant activities. Moreover, SCPE significantly prevented the increase in serum ALT and AST level in acute liver damage induced by CCl4, decreased the extent of malondialdehyde (MDA) formation in liver and elevated the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in liver. The results indicated that SCPE has strong antioxidant activities and significant protective effect against acute hepatotoxicity induced by CCl4, and have been supported by the evaluation of liver histopathology in mice. The hepatoprotective effect may be related to its free radical scavenging effect, increasing antioxidant activity and inhibiting lipid peroxidation.
authors.affiliation: Institute of Analytical Science, Shaanxi Provincial Key Lab of Electroanalytical Chemistry, Northwest University, 229 North TaiBai Road, Xi'an City 710069, China.
authors.forename: Ni
authors.initials: N
authors.lastname: Cheng
authors.forename: Naiyan
authors.initials: N
authors.lastname: Ren
authors.forename: Hui
authors.initials: H
authors.lastname: Gao
authors.forename: Xingsheng
authors.initials: X
authors.lastname: Lei
authors.forename: Jianbin
authors.initials: J
authors.lastname: Zheng
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D002252:Carbon Tetrachloride Poisoning / Q000517:prevention & control*; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis; D007502:Iron Chelating Agents / Q000494:pharmacology; D008297:Male; D051379:Mice; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000627:therapeutic use*; D011058:Pollen / Q000737:chemistry*; D032141:Schisandra / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D007502:Iron Chelating Agents; D010636:Phenols; D010936:Plant Extracts