title: In vivo bioluminescence imaging of magnetically targeted bone marrow-derived mesenchymal stem cells in skeletal muscle injury model.
abstract: The purpose of this study is to clarify the kinetics of transplanted mesenchymal stem cells (MSCs) in rat skeletal muscle injury model and the contribution of the magnetic cell delivery system to muscle injury repair. A magnetic field generator was used to apply an external magnetic force to the injury site of the tibia anterior muscle, and 1 × 10(6) MSCs labeled with ferucarbotran-protamine complexes, which were isolated from luciferase transgenic rats, were injected into the injury site. MSCs were injected with and without an external magnetic force (MSC M+ and MSC M- groups, respectively), and phosphate-buffered saline was injected into injury sites as a control. In vivo bioluminescence imaging was performed immediately after the transplantation and, at 12, 24, and 72 h, and 1 and 4 weeks post-transplantation. Also, muscle regeneration and function were histologically and electromechanically evaluated. In vivo bioluminescence imaging showed that the photon of the MSC M+ group was significantly higher than that of the MSC M- group throughout the observation period. In addition, muscle regeneration and function in the MSC M+ group was histologically and functionally better than that of the MSC M- group. The results of our study indicated that magnetic cell delivery system may be of use in directing the transplanted MSCs to the injury site to promote skeletal muscle regeneration.
authors.affiliation: Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minaki-ku, Hiroshima 734-8551, Japan. baya1021@gmail.com
authors.forename: Akihiro
authors.initials: A
authors.lastname: Nakabayashi
authors.forename: Naosuke
authors.initials: N
authors.lastname: Kamei
authors.forename: Toru
authors.initials: T
authors.lastname: Sunagawa
authors.forename: Osami
authors.initials: O
authors.lastname: Suzuki
authors.forename: Shingo
authors.initials: S
authors.lastname: Ohkawa
authors.forename: Akira
authors.initials: A
authors.lastname: Kodama
authors.forename: Goki
authors.initials: G
authors.lastname: Kamei
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Ochi
mesh_terms: D000818:Animals; D001265:Athletic Injuries / Q000503:physiopathology / Q000628:therapy*; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D008156:Luciferases / Q000235:genetics; D008163:Luminescent Measurements / Q000379:methods*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D018482:Muscle, Skeletal / Q000166:cytology / Q000293:injuries* / Q000502:physiology; D051381:Rats; D011917:Rats, Inbred Lew; D055647:Rats, Transgenic; D012038:Regeneration / Q000502:physiology
chemical_list: D008156:Luciferases