title: Effect of oxygen on the biosynthesis of flavor compound 3-methylbutanal from leucine catabolism during batch culture in Carnobacterium maltaromaticum LMA 28.
abstract: In this study, we demonstrated the effect of different dissolved oxygen concentrations (DOC) on cell growth and intracellular biosynthesis of 3-methylbutanal from leucine catabolism in Carnobacterium maltaromaticum LMA 28 during batch culture. The maximum specific growth rate was obtained in culture when DOC was controlled at 50% of air saturation. The specific consumption rates of glucose and specific production rates of lactate were higher at a DOC at 50 or 90% of air saturation. Carnobacterium maltaromaticum LMA 28 produced high quantities of 3-methylbutanal and 3-methylbutanol during culture with DOC maintained at 90%, suggesting that oxygen had a significant effect of the formation of these flavor compounds. This high formation of flavor compounds in an oxygen-rich environment was attributed to the simultaneous activation and stimulation of both α-ketoacid decarboxylase (KADC) and α-ketoacid dehydrogenase (KADH) pathways. Thus, intracellular biosynthesis of 3-methylbutanal can be controlled by modifying the DOC of the culture or food product during fermentation.
authors.affiliation: Laboratoire d'ingénierie des biomolécules, Université de Lorraine, 2 avenue de la Forêt de Haye B.P. 172, Vandoeuvre-lès-Nancy F-54505, France.
authors.forename: M I
authors.initials: MI
authors.lastname: Afzal
authors.forename: K-A
authors.initials: KA
authors.lastname: Boulahya
authors.forename: C
authors.initials: C
authors.lastname: Paris
authors.forename: S
authors.initials: S
authors.lastname: Delaunay
authors.forename: C
authors.initials: C
authors.lastname: Cailliez-Grimal
mesh_terms: D000447:Aldehydes / Q000378:metabolism*; D058491:Bacterial Load; D056573:Carnobacterium / Q000254:growth & development / Q000378:metabolism*; D002849:Chromatography, Gas; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology
chemical_list: D000447:Aldehydes; D003470:Culture Media; D019344:Lactic Acid; C032251:isovalerylaldehyde; D007930:Leucine; D005947:Glucose; D010100:Oxygen