title: Effects of body fat reduction on plasma adipocyte fatty acid-binding protein concentration in obese patients with type 1 diabetes mellitus.
abstract: OBJECTIVE
  The influence of body fat reduction on adipocyte fatty acid-binding protein (A-FABP) in obese patients with type 1 diabetes mellitus (T1DM) was investigated to examine whether it relates to the etiopathogenesis of insulin resistance (IR) and obesity.
  
  
  METHODS
  We studied 14 obese patients with T1DM and IR (42.6±9.4 years, BMI 32.4±2.1 kg/m2) and 13 non-obese control patients with T1DM (36.9±13.9 years, BMI 22.6±2.1 kg/m2). Plasma FABP was measured by ELISA and plasma free fatty acids (FFA) were measured spectrophotometrically before weight reduction, immediately after 7 days of fasting and after 21 days on a low-calorie diet. The control group was studied only after overnight fasting. Body composition was examined using bioimpedance spectroscopy. The means ± SD, T-test, one-way ANOVA and Spearman's correlation were used for statistical evaluation.
  
  
  RESULTS
  All patients tolerated the period of fasting. Obese T1DM patients lost 6.1±1.1 kg. There was a significant decrease in body mass index and body fat measured 21 days after weight reduction (p<0.05). Plasma FABP and FFA concentrations in obese T1DM patients before weight reduction were significantly higher than in controls, further increased significantly after fasting (p<0.05) and were restored thereafter. Significant positive correlations between FABP and FFA and between FABP and BMI (p<0.05) were found.
  
  
  CONCLUSIONS
  Increased plasma FABP indicates insulin resistance in obese patients with T1DM. Weight reduction in T1DM patients is associated with a desirable decrease of body fat and transiently increased FABP. This increase might be a temporary adaptation of metabolism to non-stress fasting.
authors.affiliation: 3rd Department of Medicine, Metabolic Care and Gerontology, Charles University in Hradec Králové, Czech Republic. blaha@lfhk.cuni.cz
authors.forename: Vladimir
authors.initials: V
authors.lastname: Blaha
authors.forename: Frantisek
authors.initials: F
authors.lastname: Musil
authors.forename: Alena
authors.initials: A
authors.lastname: Smahelova
authors.forename: Alena
authors.initials: A
authors.lastname: Ticha
authors.forename: Radomir
authors.initials: R
authors.lastname: Hyspler
authors.forename: Martin
authors.initials: M
authors.lastname: Haluzik
authors.forename: Jana
authors.initials: J
authors.lastname: Lesna
authors.forename: Lubos
authors.initials: L
authors.lastname: Sobotka
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D001823:Body Composition / Q000502:physiology; D003922:Diabetes Mellitus, Type 1 / Q000188:drug therapy / Q000378:metabolism*; D004038:Diet, Reducing; D005215:Fasting / Q000502:physiology; D050556:Fatty Acid-Binding Proteins / Q000097:blood*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D015309:Glucose Clamp Technique; D006801:Humans; D007004:Hypoglycemic Agents / Q000008:administration & dosage; D007328:Insulin / Q000008:administration & dosage; D007333:Insulin Resistance / Q000502:physiology; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000178:diet therapy / Q000378:metabolism*; D015431:Weight Loss / Q000502:physiology
chemical_list: C493410:FABP4 protein, human; D050556:Fatty Acid-Binding Proteins; D005230:Fatty Acids, Nonesterified; D007004:Hypoglycemic Agents; D007328:Insulin