title: Purification, crystallization and X-ray diffraction analysis of Trypanosoma congolense insect-stage surface antigen (TcCISSA).
abstract: Trypanosoma congolense is a major contributor to the vast socioeconomic devastation in sub-Saharan Africa caused by animal African trypanosomiasis. These protozoan parasites are transmitted between mammalian hosts by tsetse-fly vectors. A lack of understanding of the molecular basis of tsetse-trypanosome interactions stands as a barrier to the development of improved control strategies. Recently, a stage-specific T. congolense protein, T. congolense insect-stage surface antigen (TcCISSA), was identified that shows considerable sequence identity (>60%) to a previously identified T. brucei insect-stage surface molecule that plays a role in the maturation of infections. TcCISSA has multiple di-amino-acid and tri-amino-acid repeats in its extracellular domain, making it an especially interesting structure-function target. The predicted mature extracellular domain of TcCISSA was produced by recombinant DNA techniques, purified from Escherichia coli, crystallized and subjected to X-ray diffraction analysis; the data were processed to 2.7 Å resolution.
authors.affiliation: Department of Biochemistry and Microbiology, University of Victoria, Petch Building, 3800 Finnerty Road, Victoria, BC V8P 5C2, Canada.
authors.forename: Michelle L
authors.initials: ML
authors.lastname: Tonkin
authors.forename: Sean D
authors.initials: SD
authors.lastname: Workman
authors.forename: Brett A
authors.initials: BA
authors.lastname: Eyford
authors.forename: Bianca C
authors.initials: BC
authors.lastname: Loveless
authors.forename: Jessica L
authors.initials: JL
authors.lastname: Fudge
authors.forename: Terry W
authors.initials: TW
authors.lastname: Pearson
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Boulanger
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000737:chemistry* / Q000302:isolation & purification*; D003460:Crystallization; D018360:Crystallography, X-Ray; D007303:Insect Vectors / Q000378:metabolism; D015800:Protozoan Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D014348:Trypanosoma congolense / Q000276:immunology*; D014353:Trypanosomiasis, African / Q000276:immunology; D014961:X-Ray Diffraction
chemical_list: D000954:Antigens, Surface; D015800:Protozoan Proteins