title: Isolation and characterization of homologous TRBP cDNA for RNA interference in Penaeus monodon.
abstract: The transactivation response RNA-binding protein (TRBP) interacts with Dicer and binds to double-stranded RNA as a critical component of the RNA-induced silencing complex, which is a key complex in the RNA interference pathway. The full-length cDNA of TRBP from the tiger prawn, Penaeus monodon, (PmTRBP; 1548 bp long with a 1029 bp coding region) was isolated. The encoded polypeptide of 343 amino acids had a predicted molecular mass of 36.8 kDa. Sequence homology and phylogenetic analysis indicated that PmTRBP was evolutionarily closest to TRBP1 from Litopenaeus vannamei, with the three double-stranded RNA-binding motifs that were typical of the TRBP family. Tissue expression profile analysis by quantitative real-time reverse transcription polymerase chain reaction showed that PmTRBP1 was constitutively expressed in all the examined tissues, with a predominant expression in the lymphatic organs and with the weakest expression in the ovaries. Significantly upregulated PmTRBP1 expression was elicited by systemic injections of Staphylococcus aureus, Vibrio vulnificus, and white spot syndrome virus, thereby revealing its pathogen inducibility. Furthermore, exogenous viral nucleoside analogs (high-molecular-weight poly(I:C) dsRNAs as well as R484 single-stranded RNA) were remarkably induced PmTRBP1 transcription at 48 h and 9 h post-injection, respectively, which suggested that PmTRBP1 might function in tiger prawn antibacterial and antiviral response.
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, the South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, PR China.
authors.forename: Lishi
authors.initials: L
authors.lastname: Yang
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Li
authors.forename: Jianhua
authors.initials: J
authors.lastname: Huang
authors.forename: Falin
authors.initials: F
authors.lastname: Zhou
authors.forename: Tianfeng
authors.initials: T
authors.lastname: Su
authors.forename: Shigui
authors.initials: S
authors.lastname: Jiang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics*; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000276:immunology*; D007113:Immunity, Innate / Q000235:genetics*; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000235:genetics* / Q000276:immunology; D010802:Phylogeny; D034622:RNA Interference; D016601:RNA-Binding Proteins / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013211:Staphylococcus aureus / Q000276:immunology; D041261:Vibrio vulnificus / Q000276:immunology; D046848:White spot syndrome virus 1 / Q000276:immunology; D000099253:Trans-Activation Responsive RNA-Binding Protein
chemical_list: D060829:Arthropod Proteins; D018076:DNA, Complementary; D016601:RNA-Binding Proteins; D000099253:Trans-Activation Responsive RNA-Binding Protein