title: Isolation of Prunin from the fruit shell of Bixa orellana and the effect of β-cyclodextrin on its binding with calf thymus DNA.
abstract: Naringenin-7-O-glucoside [Prunin (Pru)] was isolated from the fruit shell of Bixa orellana L. The binding of Pru with calf thymus DNA (ctDNA) and the influence of cyclomaltoheptaose (β-cyclodextrin, β-CD) on the binding were studied by absorption and fluorescence spectroscopic techniques. The comparison of the binding modes of Pru/β-CD and ctDNA-Pru/β-CD suggested that β-CD extracted Pru from DNA for forming inclusion complex. Molecular modeling gave added support to the above results. Fluorescence microscopy was used to visualize the effect of β-CD on the bindings.
authors.affiliation: Department of Chemistry, Karunya University, Coimbatore 641114, India.
authors.forename: Sameena
authors.initials: S
authors.lastname: Yousuf
authors.forename: N
authors.initials: N
authors.lastname: Sudha
authors.forename: G
authors.initials: G
authors.lastname: Murugesan
authors.forename: Israel V M V
authors.initials: IV
authors.lastname: Enoch
mesh_terms: D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D031212:Bixaceae / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry; D046911:Macromolecular Substances / Q000737:chemistry; D008751:Methylene Blue / Q000737:chemistry; D008856:Microscopy, Fluorescence / Q000379:methods; D008958:Models, Molecular; D010695:Phlorhizin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D007202:Indicators and Reagents; D046911:Macromolecular Substances; D047392:beta-Cyclodextrins; C506622:prunin; D004247:DNA; C427203:calf thymus DNA; D010695:Phlorhizin; C031215:betadex; D008751:Methylene Blue