title: Inhibition of Penicillium expansum by an oxidative treatment.
abstract: Several oxidizing compounds such as sodium hypochlorite (NaClO) and hydrogen peroxide (H(2)O(2)) are used to control postharvest decay in fresh fruit due to their antimicrobial effects. Here, we applied these compounds in vitro, in the presence of CuSO(4), against Penicillium expansum, causal agent of apple blue mold. MICs were 50 mg L(-1) and 400 mmol L(-1) for NaClO and H(2)O(2), respectively, when these compounds were individually applied to conidia suspensions during 2 min. A combined oxidative treatment (OT) consisting on an incubation with 1 mg L(-1) NaClO and 200 mmol L(-1) H(2)O(2), in the presence of 6 mmol L(-1) CuSO(4), inhibited growth, conidial germination and fungal infectivity on apple. The fractional inhibitory concentration index for the interaction between NaClO and H(2)O(2) in the OT was 0.52 indicating a synergistic effect of the oxidizing compounds. These results suggest that the OT could be an interesting alternative for apple diseases postharvest control.
authors.affiliation: Instituto de Química Biológica, Química y Farmacia and Departamento Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas, UNT, Chacabuco 461, CP T4000ILI, Tucumán, Argentina.
authors.forename: Luciana
authors.initials: L
authors.lastname: Cerioni
authors.forename: María de Los Ángeles
authors.initials: Mde L
authors.lastname: Lazarte
authors.forename: Josefina María
authors.initials: JM
authors.lastname: Villegas
authors.forename: Luisa
authors.initials: L
authors.lastname: Rodríguez-Montelongo
authors.forename: Sabrina Inés
authors.initials: SI
authors.lastname: Volentini
mesh_terms: D015536:Down-Regulation / Q000187:drug effects; D005519:Food Preservation / Q000295:instrumentation / Q000379:methods*; D005520:Food Preservatives / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010407:Penicillium / Q000187:drug effects* / Q000254:growth & development*; D012973:Sodium Hypochlorite / Q000494:pharmacology
chemical_list: D005520:Food Preservatives; D016877:Oxidants; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite