title: Within and between seasonal changes of detoxifying capabilities of Cameraria ohridella (Lepidoptera : Gracillariidae) larvae.
abstract: Cameraria ohridella is an invasive leaf miner, a severe pest of horse chestnut trees. Chemical control needs recognition of intrinsic metabolic capability to cope with external stressing factors. Our tasks were to check annual effects of generation, and host tree age on detoxifying abilities of the last larval stage. Activities of CAT, SOD, GSTPX, GST CarE and AChE were assayed in the midgut of larvae from two localities during 3 years and three generations, annually. Activities of GSTPX and GST were high, but CAT and SOD were low in relation to other lepidopteran larvae. In general, the second generation larvae had the highest activity, indicating an effective defence against allelochemicals produced by the host. Effects of host tree age were significant for SOD, GSTPX and CarE activities. Significant annual differences on enzyme profiles of CAT, SOD, CarE and AChE reflected differences of temperature and precipitation between consecutive years.
authors.affiliation: Department of Animal Physiology and Ecotoxicology, University of Silesia, Bankowa 9, Katowice, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Zaak
authors.forename: Paweł
authors.initials: P
authors.lastname: Migula
authors.forename: Dominika
authors.initials: D
authors.lastname: Stygar
authors.forename: Bogdan
authors.initials: B
authors.lastname: Doleżych
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Michalczyk
mesh_terms: D031319:Aesculus; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D008658:Inactivation, Metabolic / Q000502:physiology*; D007413:Intestinal Mucosa / Q000378:metabolism; D007814:Larva / Q000201:enzymology / Q000502:physiology*; D007915:Lepidoptera / Q000502:physiology*; D010675:Pheromones / Q000378:metabolism*; D018515:Plant Leaves / Q000469:parasitology; D011044:Poland; D012621:Seasons
chemical_list: D000975:Antioxidants; D010675:Pheromones