title: N-acetylglucosamine biofunctionalized CdSeTe quantum dots as fluorescence probe for specific protein recognition.
abstract: Hsp70 proteins are implicated in resistance to chemotherapy in cancers, the detection of which is important for cancer treatment and prognosis. In this work, we report the study on the detection of specific intracellular target protein in fixed cells using GlcNAc-conjugated CdSeTe QDs. The QDs were coupled with Con A via a carbodiimide reaction and then were further assembled with GlcNAc by lectin-carbohydrate interaction between Con A and GlcNAc. The obtained QDs-Con A-GlcNAc conjugates have an emission wavelength at 650 nm that is close to the near-infrared (NIR) regions and a specific recognition for Hsp70. These results show that the QDs-Con A-GlcNAc probe can be a promising tool for direct localization of the Hsp70 protein.
authors.affiliation: State Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, PR China.
authors.forename: Fang-Fang
authors.initials: FF
authors.lastname: Cheng
authors.forename: Guo-Xi
authors.initials: GX
authors.lastname: Liang
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Shen
authors.forename: Rohit Kumar
authors.initials: RK
authors.lastname: Rana
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhu
mesh_terms: D000117:Acetylglucosamine / Q000737:chemistry / Q000378:metabolism*; D002104:Cadmium / Q000737:chemistry; D045744:Cell Line, Tumor; D003208:Concanavalin A / Q000737:chemistry / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis* / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D045663:Quantum Dots; D012643:Selenium / Q000737:chemistry; D013691:Tellurium / Q000737:chemistry
chemical_list: D018840:HSP70 Heat-Shock Proteins; D002104:Cadmium; D003208:Concanavalin A; D012643:Selenium; D013691:Tellurium; D000117:Acetylglucosamine