title: Behavior of lignin-binding cellulase in the presence of fresh cellulosic substrate.
abstract: A model lignin-binding cellulase was prepared from Trichoderma reesei cellulase and lignocresol, which was synthesized from softwood or hardwood lignin. Filter paper was incubated with the lignocresol-cellulase complex, and it was observed that only a limited amount of cellulase migrated to the filter paper. The cellulase adsorption isotherms for the lignocresols and filter paper were fitted to a Langmuir absorption model, and the determined Langmuir constants were as follows: softwood lignocresol>hardwood lignocresol>>filter paper. The calculations demonstrated that lignin-binding cellulase can potentially be recovered by the addition of a sufficient quantity of cellulosic substrate. As a result, the lignocresol-binding cellulase is highly stable and lignocresol can potentially be used for immobilizing cellulase in the active state.
authors.affiliation: Graduate School of Bioresources, Mie University, Tsu, Mie 514-8507, Japan. nonaka@bio.mie-u.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nonaka
authors.forename: Ai
authors.initials: A
authors.lastname: Kobayashi
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Funaoka
mesh_terms: D000327:Adsorption / Q000187:drug effects; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000494:pharmacology*; D029780:Chamaecyparis / Q000737:chemistry; D005052:Eucalyptus / Q000737:chemistry; D007700:Kinetics; D008031:Lignin / Q000378:metabolism*; D013379:Substrate Specificity / Q000187:drug effects; D013696:Temperature; D014242:Trichoderma / Q000187:drug effects / Q000201:enzymology*
chemical_list: D002482:Cellulose; D008031:Lignin; D002480:Cellulase