title: Determination of the electrophoretic pattern of somatic and excretory-secretory proteins of Ligula intestinalis parasite in spirlin (Alburnoides bipunctatus).
abstract: Ligula intestinalis parasite is a cestodes that causes remarkable damages to fish.   It is also of prime importance in economic and hygienic aspects. SDS-PAGE and western   blotting are the methods that can be used to determine the electerophoretic pattern of   somatic and excretory-secretory proteins of parasites. In this study, after obtaining the   plerocercoidal stage of this parasite from the spirlin (Alburnoides bipunctatus), its somatic   proteins were prepared using ultrasonicae, and excretory-secretory proteins were prepared   using the PBS solution. After protein assay, which included using the Bradford method and   then SDS-PAGE on these two antigenic solutions, 5 protein bands of 26, 33, 38, 58, 70kDa in   somatic antigens, and 7 bands of 25, 28, 33, 43, 49, 60, 70kDa in excretory-secretory antigens   were observed. After western blotting on both antigens and adding the primary antibody (the   sera of infected fish) and then the secondary antibody (Rabbit Anti-fish Polyclonal Antibody   Conjugated from Abnova Corporation) no band was seen in excretory-secretory antigen. And   only in the 55kDa band of somatic antigen, a positive response, in comparison of fish positive   serum was observed.
authors.affiliation: Department of Veterinary Parasitology, Islamic Azad University, Babol-Branch, Iran. m_halimi82@yahoo.com
authors.forename: M R
authors.initials: MR
authors.lastname: Youssefi
authors.forename: S M
authors.initials: SM
authors.lastname: Hosseinifard
authors.forename: M
authors.initials: M
authors.lastname: Halimi
authors.forename: S
authors.initials: S
authors.lastname: Kordafshari
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002589:Cestoda / Q000737:chemistry* / Q000302:isolation & purification*; D003530:Cyprinidae / Q000469:parasitology*; D004591:Electrophoresis, Polyacrylamide Gel; D015801:Helminth Proteins / Q000032:analysis* / Q000737:chemistry; D008970:Molecular Weight; D020543:Proteome / Q000032:analysis*
chemical_list: D015801:Helminth Proteins; D020543:Proteome