title: N- and o-glycosylation of a commercial bovine whey protein product.
abstract: Bovine whey protein products are used as a base ingredient in infant formulas to optimize the amino acid pattern to a more human-like composition. Although the protein composition of bovine milk has been studied in detail, glycosylation details of commercial whey protein products are missing. To this end, both the N- and O-glycans of such a protein concentrate were sequentially released, the N-glycans enzymatically and the O-glycans chemically (reducing and nonreducing conditions). For the structural analysis of the N- and O-glycans a combination of MALDI-TOF-MS, one-dimensional (1)H NMR spectroscopy,  Wisteria floribunda  agglutinin affinity chromatography, HPAEC-PAD profiling, and HPLC-FD profiling (2-aminobenzamide derivatives), together with exoglycosidase treatments, were used. A mixture of over 60 N-glycans and 10 O-glycans was characterized, giving a detailed insight into the glycosylation of a bovine whey protein product, Deminal 90, which is applied as an ingredient for infant formulas.
authors.affiliation: Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen , Nijenborgh 7, NL-9747 AG Groningen, The Netherlands.
authors.forename: Sander S
authors.initials: SS
authors.lastname: van Leeuwen
authors.forename: Ruud J W
authors.initials: RJ
authors.lastname: Schoemaker
authors.forename: Christel J A M
authors.initials: CJ
authors.lastname: Timmer
authors.forename: Johannis P
authors.initials: JP
authors.lastname: Kamerling
authors.forename: Lubbert
authors.initials: L
authors.lastname: Dijkhuizen
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002417:Cattle; D006026:Glycoside Hydrolases / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D007223:Infant; D041943:Infant Formula / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008894:Milk Proteins / Q000737:chemistry* / Q000378:metabolism; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D000067816:Whey Proteins
chemical_list: D008894:Milk Proteins; D011134:Polysaccharides; D000067816:Whey Proteins; D006026:Glycoside Hydrolases