title: Small ubiquitin-like modifier conjugating enzyme with active site mutation acts as dominant negative inhibitor of SUMO conjugation in arabidopsis(F).
abstract: Small ubiquitin-like modifier (SUMO) conjugation affects a broad range of processes in plants, including growth, flower initiation, pathogen defense, and responses to abiotic stress. Here, we investigate in vivo and in vitro a SUMO conjugating enzyme with a Cys to Ser change in the active site, and show that it has a dominant negative effect. In planta expression significantly perturbs normal development, leading to growth retardation, early flowering and gene expression changes. We suggest that the mutant protein can serve as a probe to investigate sumoylation, also in plants for which poor genetic infrastructure precludes analysis via loss-of-function mutants.
authors.affiliation: Department of Biochemistry and Cell Biology, Max F. Perutz Laboratories, Center for Molecular Biology, University of Vienna, A-1030 Vienna, Austria.
authors.forename: Konstantin
authors.initials: K
authors.lastname: Tomanov
authors.forename: Christian
authors.initials: C
authors.lastname: Hardtke
authors.forename: Ruchika
authors.initials: R
authors.lastname: Budhiraja
authors.forename: Rebecca
authors.initials: R
authors.lastname: Hermkes
authors.forename: George
authors.initials: G
authors.lastname: Coupland
authors.forename: Andreas
authors.initials: A
authors.lastname: Bachmair
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000378:metabolism*; D015153:Blotting, Western; D009154:Mutation; D025841:Small Ubiquitin-Related Modifier Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D058207:Sumoylation; D014158:Transcription, Genetic; D025801:Ubiquitin / Q000378:metabolism*
chemical_list: D025841:Small Ubiquitin-Related Modifier Proteins; D025801:Ubiquitin