title: Label-free DNA detection using the narrow side of funnel-type etched nanopores.
abstract: A sensitive; simple and label-free method for DNA detection is presented. Irradiated polyethylene terephthalate foils were etched to present conical tracks at a density of approximately 5×10⁷ tracks per cm². Probe DNA was immobilized at the narrow opening of the tracks (2-4 nm diameter). The presence of target DNA resulted in changes in both conductance and rectification ratios in a concentration-dependent manner. DNA concentrations as low as 2 nM could be detected using this simple two-step detection scheme.
authors.affiliation: Avram and Stella Goldstein-Goren Department of Biotechnology Engineering and the Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, PO Box 653, Beer-Sheva 84105, Israel.
authors.forename: Yohai
authors.initials: Y
authors.lastname: Mandabi
authors.forename: Dietmar
authors.initials: D
authors.lastname: Fink
authors.forename: Lital
authors.initials: L
authors.lastname: Alfonta
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D015342:DNA Probes / Q000737:chemistry; D058608:Nanopores; D011093:Polyethylene Terephthalates / Q000737:chemistry
chemical_list: D015342:DNA Probes; D011093:Polyethylene Terephthalates; D004247:DNA