title: Microfluidic cell sorter (μFCS) for on-chip capture and analysis of single cells.
abstract: A low-cost and rapid microfluidic cell sorter (μFCS) for circulating tumor cell (CTC) detection, culture and analyses is developed. Based on size separation and molecular characterization, μFCS efficiently enriches CTCs from unprocessed whole blood, allows on-chip culture and molecular profiling, and provides cell retrieval for subsequent analyses. The potential clinical application of the technology is demonstrated by capturing and genetically analyzing CTCs in tumor-bearing mice.
authors.affiliation: Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114, USA.
authors.forename: Jaehoon
authors.initials: J
authors.lastname: Chung
authors.forename: Huilin
authors.initials: H
authors.lastname: Shao
authors.forename: Thomas
authors.initials: T
authors.lastname: Reiner
authors.forename: David
authors.initials: D
authors.lastname: Issadore
authors.forename: Ralph
authors.initials: R
authors.lastname: Weissleder
authors.forename: Hakho
authors.initials: H
authors.lastname: Lee
mesh_terms: D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D009360:Neoplastic Cells, Circulating / Q000473:pathology*