title: Neisseria gonorrhoeae strain with high-level resistance to spectinomycin due to a novel resistance mechanism (mutated ribosomal protein S5) verified in Norway.
abstract: Gonorrhea may become untreatable, and new treatment options are essential. Verified resistance to spectinomycin is exceedingly rare. However, we describe a high-level spectinomycin-resistant (MIC, >1,024 μg/ml) Neisseria gonorrhoeae strain from Norway with a novel resistance mechanism. The resistance determinant was a deletion of codon 27 (valine) and a K28E alteration in the ribosomal protein 5S. The traditional spectinomycin resistance gene (16S rRNA) was wild type. Despite this exceedingly rare finding, spectinomycin available for treatment of ceftriaxone-resistant urogenital gonorrhea would be very valuable.
authors.affiliation: WHO Collaborating Centre for Gonorrhoea and other STIs, Swedish Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Microbiology, Örebro University Hospital, Örebro, Sweden. magnus.unemo@orebroll.se
authors.forename: Magnus
authors.initials: M
authors.lastname: Unemo
authors.forename: Daniel
authors.initials: D
authors.lastname: Golparian
authors.forename: Vegard
authors.initials: V
authors.lastname: Skogen
authors.forename: Anne Olaug
authors.initials: AO
authors.lastname: Olsen
authors.forename: Harald
authors.initials: H
authors.lastname: Moi
authors.forename: Gaute
authors.initials: G
authors.lastname: Syversen
authors.forename: Stig Ove
authors.initials: SO
authors.lastname: Hjelmevoll
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D024881:Drug Resistance, Bacterial / Q000235:genetics; D006069:Gonorrhea / Q000188:drug therapy; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009344:Neisseria gonorrhoeae / Q000145:classification / Q000187:drug effects* / Q000235:genetics*; D009664:Norway; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012269:Ribosomal Proteins / Q000235:genetics*; D016415:Sequence Alignment; D000198:Spectinomycin / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D012336:RNA, Ribosomal, 16S; D012269:Ribosomal Proteins; C030831:ribosomal protein S5; D000198:Spectinomycin