title: Effect of the addition of IGF-I and vitamin E to stored boar semen.
abstract: The objective of this study was to evaluate the addition of IGF-I to pig insemination doses stored at 15°C, in conjunction with the addition of different amounts of vitamin E (α-tocopherol). Semen samples (n = 12) from four boars were treated by the addition of different concentrations of vitamin E, ranging up to 400 μg/ml. Immediately after processing and after the doses had been stored at 15°C for 24 or 72 h, samples were warmed at 37°C and 30 ng/ml of IGF-I was added. The assessments were made after 10 and 120 min of IGF-I addition. There was a minor effect of the vitamin E added before cooling and IGF-I added after storage on sperm quality. The addition of 400 μg/ml of vitamin E to diluted semen reduced (P < 0.01) the malondialdehyde (MDA) production in boar semen stored at 15°C for 72 h, regardless of the addition of IGF-I as additive during a 120 min incubation period at 37°C. In these conditions, IGF-I also reduced (P < 0.05) the MDA production in semen samples without addition of vitamin E. IGF-I in the presence of vitamin E reduced (P = 0.03) the glucose intake in freshly diluted boar semen samples before cooling. It was concluded that the addition of 400 μg/ml of vitamin E reduces the MDA production in boar semen stored at 15°C for 72 h, regardless of the presence of IGF-I additive. The addition of IGF-I in doses stored for 72 h with vitamin E ensures higher sperm motility after 120 min of incubation at 37°C.
authors.affiliation: Department of Veterinary Science, Federal University of Lavras, Lavras, Minas Gerais, Brazil.
authors.forename: M F B
authors.initials: MF
authors.lastname: Mendez
authors.forename: M G
authors.initials: MG
authors.lastname: Zangeronimo
authors.forename: L G P
authors.initials: LG
authors.lastname: Rocha
authors.forename: B G
authors.initials: BG
authors.lastname: Faria
authors.forename: B A
authors.initials: BA
authors.lastname: Pereira
authors.forename: C D
authors.initials: CD
authors.lastname: Fernandes
authors.forename: B R
authors.initials: BR
authors.lastname: Chaves
authors.forename: L D S
authors.initials: LD
authors.lastname: Murgas
authors.forename: R V
authors.initials: RV
authors.lastname: Sousa
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D008297:Male; D012661:Semen / Q000187:drug effects*; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology; D013552:Swine / Q000502:physiology*; D024502:alpha-Tocopherol / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D007334:Insulin-Like Growth Factor I; D024502:alpha-Tocopherol