title: Histomorphometric and immunohistochemical study of the goat omasum during prenatal development.
abstract: This work studies the morphological changes taking place in the goat omasum during prenatal development, using scanning electron microscope, light microscopy and immunohistochemical analysis. A total of 140 goat embryos and fetuses were used, from the first stages of prenatal life until birth. Differentiation of the omasum as a separate compartment of the primitive gastric tube was observed at 35 days of prenatal life ([crown-rump length (CRL)] 3 cm, 23% gestation). By 38 days (CRL 4.3 cm, 25% gestation) the omasal wall comprised three layers: an internal epithelial layer, a middle layer of pluripotential blastemic tissue and an external layer or serosa. Omasal laminae appeared in the following order: primary at 38 days (CRL 4.3 cm, 25% gestation), secondary at 50 days (CRL 7.7 cm, 33% gestation), tertiary at 59 days (CRL 12 cm, 39% gestation) and quaternary at 64 days (CRL 13.5 cm, 43% gestation). Neuroendocrine cells were detected by synaptophysin (SYP) at 52 days (CRL 8 cm, 35% gestation), while glial cell markers (glial fibrillary acidic protein - GFAP, and vimentin-VIM) were observed at 64 days (CRL 13.5 cm, 43% gestation) and 38 days (CRL 4.3 cm, 25% gestation), respectively. Sympathetic and parasympathetic nerve fibers and nerve bodies were detected via neuropeptide Y (NPY) and vasoactive intestinal polypeptide (VIP) at 95 days (CRL 20 cm, 63% gestation). In conclusion, prenatal development of the omasum - like that of the rumen - appears to take place somewhat earlier in goats than in sheep or cattle, but at a similar stage to that reported in deer.
authors.affiliation: Department of Veterinary Histology, Faculty of Veterinary Medicine, University of Extremadura, Cáceres, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Garcia
authors.forename: Javier
authors.initials: J
authors.lastname: Masot
authors.forename: Antonio
authors.initials: A
authors.lastname: Franco
authors.forename: Antonio
authors.initials: A
authors.lastname: Gazquez
authors.forename: Eloy
authors.initials: E
authors.lastname: Redondo
mesh_terms: D000320:Adrenergic Fibers / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000378:metabolism; D018568:Crown-Rump Length; D004622:Embryo, Mammalian / Q000196:embryology* / Q000378:metabolism / Q000648:ultrastructure; D005865:Gestational Age; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006041:Goats / Q000196:embryology* / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D055099:Neuroendocrine Cells / Q000166:cytology / Q000378:metabolism; D009478:Neuropeptide Y / Q000378:metabolism; D009851:Omasum / Q000196:embryology* / Q000378:metabolism / Q000648:ultrastructure; D017777:Parasympathetic Fibers, Postganglionic / Q000378:metabolism; D013045:Species Specificity; D016708:Synaptophysin / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
chemical_list: D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein; D009478:Neuropeptide Y; D016708:Synaptophysin; D014746:Vimentin; D014660:Vasoactive Intestinal Peptide