title: Multiple kisspeptin receptors in early osteichthyans provide new insights into the evolution of this receptor family.
abstract: Deorphanization of GPR54 receptor a decade ago led to the characterization of the kisspeptin receptor (Kissr) in mammals and the discovery of its major role in the brain control of reproduction. While a single gene encodes for Kissr in eutherian mammals including human, other vertebrates present a variable number of Kissr genes, from none in birds, one or two in teleosts, to three in an amphibian, xenopus. In order to get more insight into the evolution of Kissr gene family, we investigated the presence of Kissr in osteichthyans of key-phylogenetical positions: the coelacanth, a representative of early sarcopterygians, the spotted gar, a non-teleost actinopterygian, and the European eel, a member of an early group of teleosts (elopomorphs). We report the occurrence of three Kissr for the first time in a teleost, the eel. As measured by quantitative RT-PCR, the three eel Kissr were differentially expressed in the brain-pituitary-gonadal axis, and differentially regulated in experimentally matured eels, as compared to prepubertal controls. Subfunctionalisation, as shown by these differences in tissue distribution and regulation, may have represented significant evolutionary constraints for the conservation of multiple Kissr paralogs in this species. Furthermore, we identified four Kissr in both coelacanth and spotted gar genomes, providing the first evidence for the presence of four Kissr in vertebrates. Phylogenetic and syntenic analyses supported the existence of four Kissr paralogs in osteichthyans and allowed to propose a clarified nomenclature of Kissr (Kissr-1 to -4) based on these paralogs. Syntenic analysis suggested that the four Kissr paralogs arose through the two rounds of whole genome duplication (1R and 2R) in early vertebrates, followed by multiple gene loss events in the actinopterygian and sarcopterygian lineages. Due to gene loss there was no impact of the teleost-specific whole genome duplication (3R) on the number of Kissr paralogs in current teleosts.
authors.affiliation: Muséum National d'Histoire Naturelle, Research Unit BOREA, Biology of Aquatic Organisms and Ecosystems, CNRS 7208- IRD207- UPMC, Paris, France.
authors.forename: Jérémy
authors.initials: J
authors.lastname: Pasquier
authors.forename: Anne-Gaëlle
authors.initials: AG
authors.lastname: Lafont
authors.forename: Shan-Ru
authors.initials: SR
authors.lastname: Jeng
authors.forename: Marina
authors.initials: M
authors.lastname: Morini
authors.forename: Ron
authors.initials: R
authors.lastname: Dirks
authors.forename: Guido
authors.initials: G
authors.lastname: van den Thillart
authors.forename: Jonna
authors.initials: J
authors.lastname: Tomkiewicz
authors.forename: Hervé
authors.initials: H
authors.lastname: Tostivint
authors.forename: Ching-Fong
authors.initials: CF
authors.lastname: Chang
authors.forename: Karine
authors.initials: K
authors.lastname: Rousseau
authors.forename: Sylvie
authors.initials: S
authors.lastname: Dufour
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D019143:Evolution, Molecular; D005399:Fishes / Q000235:genetics* / Q000254:growth & development; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D016678:Genome / Q000235:genetics; D006801:Humans; D059648:Kisspeptins / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics*; D026801:Synteny
chemical_list: D018076:DNA, Complementary; D059648:Kisspeptins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface