title: Carbohydrate microarrays.
abstract: In the last decade, carbohydrate microarrays have been core technologies for analyzing carbohydrate-mediated recognition events in a high-throughput fashion. A number of methods have been exploited for immobilizing glycans on the solid surface in a microarray format. This microarray-based technology has been widely employed for rapid analysis of the glycan binding properties of lectins and antibodies, the quantitative measurements of glycan-protein interactions, detection of cells and pathogens, identification of disease-related anti-glycan antibodies for diagnosis, and fast assessment of substrate specificities of glycosyltransferases. This review covers the construction of carbohydrate microarrays, detection methods of carbohydrate microarrays and their applications in biological and biomedical research.
authors.affiliation: National Creative Research Initiative Center for Biofunctional Molecules, Department of Chemistry, Yonsei University, Seoul 120-749, Korea.
authors.forename: Sungjin
authors.initials: S
authors.lastname: Park
authors.forename: Jeffrey C
authors.initials: JC
authors.lastname: Gildersleeve
authors.forename: Ola
authors.initials: O
authors.lastname: Blixt
authors.forename: Injae
authors.initials: I
authors.lastname: Shin
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis; D002241:Carbohydrates / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000378:metabolism; D046228:Microarray Analysis; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D015415:Biomarkers; D002241:Carbohydrates; D037102:Lectins; D011134:Polysaccharides; D014764:Viral Proteins