title: Induction of double ovulation in mares using deslorelin acetate.
abstract: This study aimed to determine whether deslorelin acetate could induce double ovulation in mares. In Experiment 1, eight mares were treated with prostaglandin on Day 8 (D8) after ovulation, then treated with saline or with 100 μg of a controlled-release formulation of deslorelin acetate vehicle intramuscularly (IM) every 12h from D8 after ovulation until at least two follicles reached 33 mm. At this time, ovulation was induced with 2500 IU of hCG. Artificial insemination was performed 24h after induction, and embryos were collected on the eighth day after ovulation was first detected. In Experiment 2, 112 estrous cycles in 56 mares were studied. In this experiment, the deslorelin acetate protocol was initiated only in mares that achieved a follicle with a diameter of at least 25 mm and at least one second follicle with a diameter≥20mm was detected, at which time 100 μg deslorelin acetate or saline was administered IM every 12h. The other procedures were similar to those described in Experiment 1. The variables studied were analyzed using Student's t-test and Fisher's exact test. In Experiment 1, only two mares in deslorelin group having second follicles of 20-25 mm on responded with double ovulation. In the second experiment, 82% of treated mares responded with double ovulation, and the embryo recovery per estrous cycle was 1.12 and 0.57 in the group treated with deslorelin acetate and the control group, respectively (P<0.05). Deslorelin acetate is effective in inducing double ovulation in mares using the protocol proposed. On average, it allows for the recovery of one embryo by uterine flushing.
authors.affiliation: College of Veterinary Medicine e Animal Science, Department of Animal Reproduction and Veterinary Radiology, UNESP University of Sao Paulo State, Botucatu, SP. Brazil.
authors.forename: J F
authors.initials: JF
authors.lastname: Nagao
authors.forename: J R
authors.initials: JR
authors.lastname: Neves Neto
authors.forename: F O
authors.initials: FO
authors.lastname: Papa
authors.forename: M A
authors.initials: MA
authors.lastname: Alvarenga
authors.forename: C P
authors.initials: CP
authors.lastname: Freitas-Dell'Aqua
authors.forename: J A Junior
authors.initials: JA
authors.lastname: Dell'Aqua
mesh_terms: D000818:Animals; D004624:Embryo Transfer / Q000662:veterinary; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D006736:Horses / Q000502:physiology*; D006080:Ovarian Follicle / Q000187:drug effects / Q000502:physiology; D010060:Ovulation / Q000187:drug effects*; D010062:Ovulation Induction / Q000379:methods / Q000662:veterinary*; D017329:Triptorelin Pamoate / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D017329:Triptorelin Pamoate; C066438:deslorelin