title: Genotoxic Potential of Reactive Oxygen Species (Ros), Lipid Peroxidation and DNA Repair Enzymes (Fpg and Endo III) in Alloxan Injected Diabetic Rats.
abstract: Diabetes is a chronic metabolic syndrome due to insulin deficiency and is associated with increased oxidative stress in vivo.  Oxidative stress including, increased production of reactive oxygen species (ROS) in vivo, can lead to cellular biomolecule damage. Such damage has been suggested to contribute to the pathogenesis of diabetes mellitus. Genotoxicity induced by ROS in diabetic rats, was estimated by measuring DNA single strand breaks and double strand breaks by standard comet assay/ single cell gel electrophoresis (SCGE). To find out whether DNA lesions were caused due to oxidative stress, combination of bacterial DNA repair enzymes which convert base damage to breaks are used.  Formamidoaminopyrimidine glycosylase (Fpg) and Endonuclese (Endo III)] recognize oxidized purines and oxidized pyrimidines, respectively, were used in modified comet assay. Significant increase in DNA strand breaks in terms of DNA damage index were observed in diabetic rat lymphocytes in modified comet assay. The involvement of oxidative stress was also examined by estimation of thiobarbituric acid reactive substances (TBARS) in diabetic rats. The levels of TBARS, reactive oxygen species (ROS) namely, hydrogen peroxide, superoxide and nitrate/nitrite anion were also increased in diabetic rats that further shows the involvement of oxidative stress in ROS induced DNA damage. The results of the present study show genotoxic potential of ROS in diabetic rats.
authors.affiliation: Clinical Research Center, NIDDK, National Institutes of Health, Bethesda, Maryland, USA. yadavhariom@gmail.com.
authors.forename: Santosh Kumar
authors.initials: SK
authors.lastname: Yaduvanshi
authors.forename: Nalini
authors.initials: N
authors.lastname: Srivastava
authors.forename: G B K S
authors.initials: GB
authors.lastname: Prasad
authors.forename: Mukesh
authors.initials: M
authors.lastname: Yadav
authors.forename: Shalini
authors.initials: S
authors.lastname: Jain
authors.forename: Hariom
authors.initials: H
authors.lastname: Yadav