title: Comparative antitumor effect of preventive versus therapeutic vaccines employing B16 melanoma cells genetically modified to express GM-CSF and B7.2 in a murine model.
abstract: Cancer vaccines have always been a subject of gene therapy research. One of the most successful approaches has been working with genetically modified tumor cells. In this study, we describe our approach to achieving an immune response against a murine melanoma model, employing B16 tumor cells expressing GM-CSF and B7.2. Wild B16 cells were injected in C57BL6 mice to cause the tumor. Irradiated B16 cells transfected with GM-CSF, B7.2, or both, were processed as a preventive and therapeutic vaccination. Tumor volumes were measured and survival curves were obtained. Blood samples were taken from mice, and IgGs of each treatment group were also measured. The regulatory T&#160;cells (Treg) of selected groups were quantified using counts of images taken by confocal microscopy.
  
  
  RESULTS
  one hundred percent survival was achieved by preventive vaccination with the group of cells transfected with p2F_GM-CSF. Therapeutic vaccination achieved initial inhibition of tumor growth but did not secure overall survival of the animals. Classical Treg cells did not vary among the different groups in this therapeutic vaccination model.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, University of Valencia, Avda. Blasco Ibáñez, 15, Valencia 46010, Spain. matasantonio@hotmail.com
authors.forename: Antonio
authors.initials: A
authors.lastname: Miguel
authors.forename: María José
authors.initials: MJ
authors.lastname: Herrero
authors.forename: Luis
authors.initials: L
authors.lastname: Sendra
authors.forename: Rafael
authors.initials: R
authors.lastname: Botella
authors.forename: Rosa
authors.initials: R
authors.lastname: Algás
authors.forename: Maria
authors.initials: M
authors.lastname: Sánchez
authors.forename: Salvador F
authors.initials: SF
authors.lastname: Aliño
mesh_terms: D000818:Animals; D051940:B7-2 Antigen / Q000235:genetics* / Q000276:immunology; D019496:Cancer Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D003602:Cytotoxicity, Immunologic; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics* / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology / Q000517:prevention & control*; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D016019:Survival Analysis; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D014162:Transfection
chemical_list: D051940:B7-2 Antigen; D019496:Cancer Vaccines; C497965:Cd86 protein, mouse; D007074:Immunoglobulin G; D016178:Granulocyte-Macrophage Colony-Stimulating Factor