title: Effect of condensed tannins on bovine rumen protist diversity based on 18S rRNA gene sequences.
abstract: Molecular diversity of protists from bovine rumen fluid incubated with condensed tannins of Leucaena leucocephala hybrid-Rendang at 20 mg/500 mg dry matter (treatment) or without condensed tannins (control) was investigated using 18S rRNA gene library. Clones from the control library were distributed within nine genera, but clones from the condensed tannin treatment clone library were related to only six genera. Diversity estimators such as abundance-based coverage estimation and Chao1 showed significant differences between the two libraries, although no differences were found based on Shannon-Weaver index and Libshuff.
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, 43400, UPM Serdang, Malaysia.
authors.forename: Hui Yin
authors.initials: HY
authors.lastname: Tan
authors.forename: Chin Chin
authors.initials: CC
authors.lastname: Sieo
authors.forename: Norhani
authors.initials: N
authors.lastname: Abdullah
authors.forename: Juan Boo
authors.initials: JB
authors.lastname: Liang
authors.forename: Xiao Dan
authors.initials: XD
authors.lastname: Huang
authors.forename: Yin Wan
authors.initials: YW
authors.lastname: Ho
mesh_terms: D000818:Animals; D001105:Archaea / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D044822:Biodiversity; D002417:Cattle; D019641:DNA, Archaeal / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D007887:Fabaceae / Q000737:chemistry; D015723:Gene Library; D014644:Genetic Variation; D008697:Methane / Q000378:metabolism*; D008969:Molecular Sequence Data; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012417:Rumen / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013634:Tannins / Q000494:pharmacology*
chemical_list: D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D013634:Tannins; D008697:Methane