title: Establishment of five canine lymphoma cell lines and tumor formation in a xenotransplantation model.
abstract: Five novel, canine lymphoma cell lines (Ema, CLC, CLK, Nody-1 and UL-1) were established from dogs suffering from lymphoma and characterized in vitro and in vivo. All cell lines, except CLC, were characterized with T-cell phenotypes, by flow cytometric analysis and polymerase chain reaction for antigen receptor rearrangement. Cell proliferation rates and transcriptional levels of MYC, PTEN, KIT and FLT3 varied between each cell line. Intraperitoneal xenotransplantation of Ema, CLC, Nody-1 and UL-1 lymphoma cell lines into NOD/SCID mice induced ascites, intraperitoneal tumors and severe infiltration of lymphoma cells into the pancreas and mesentery. Establishment of novel canine lymphoma cell lines with different characteristics is critical for elucidating the pathophysiology of canine lymphoma and improving current therapies.
authors.affiliation: Laboratory of Veterinary Internal Medicine, The United Graduate School of Veterinary Science, Yamaguchi University, Yoshida, Yamaguchi, Japan.
authors.forename: Saori
authors.initials: S
authors.lastname: Umeki
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ema
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Suzuki
authors.forename: Masahito
authors.initials: M
authors.lastname: Kubo
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Hayashi
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Okamura
authors.forename: Jumpei
authors.initials: J
authors.lastname: Yamazaki
authors.forename: Hajime
authors.initials: H
authors.lastname: Tsujimoto
authors.forename: Kenji
authors.initials: K
authors.lastname: Tani
authors.forename: Hiroko
authors.initials: H
authors.lastname: Hiraoka
authors.forename: Masaru
authors.initials: M
authors.lastname: Okuda
authors.forename: Takuya
authors.initials: T
authors.lastname: Mizuno
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004283:Dog Diseases / Q000235:genetics / Q000473:pathology*; D004285:Dogs; D005260:Female; D016130:Immunophenotyping / Q000662:veterinary; D008223:Lymphoma / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D012334:RNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D013047:Specific Pathogen-Free Organisms; D014183:Transplantation, Heterologous / Q000662:veterinary
chemical_list: D012334:RNA, Neoplasm; D011948:Receptors, Antigen, T-Cell