title: In vitro assessment of thyroid hormone disrupting activities in drinking water sources along the Yangtze River.
abstract: The thyroid hormone disrupting activities of drinking water sources from the lower reaches of Yangtze River were examined using a reporter gene assay based on African green monkey kidney fibroblast (CV-1) cells. None of the eleven tested samples showed thyroid receptor (TR) agonist activity. Nine water samples exhibited TR antagonist activities with the equivalents referring to Di-n-butyl phthalate (DNBP) (TR antagonist activity equivalents, ATR-EQ(50)s) ranging from 6.92 × 10(1) to 2.85 × 10(2) μg DNBP/L. The ATR-EQ(50)s and TR antagonist equivalent ranges (ATR-EQ(30-80) ranges) for TR antagonist activities indicated that the water sample from site WX-8 posed the greatest health risks. The ATR-EQ(80)s of the water samples ranging from 1.56 × 10(3) to 6.14 × 10(3) μg DNBP/L were higher than the NOEC of DNBP. The results from instrumental analysis showed that DNBP might be responsible for the TR antagonist activities in these water samples. Water sources along Yangtze River had thyroid hormone disrupting potential.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, People's Republic of China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Hu
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.forename: Fengxian
authors.initials: F
authors.lastname: Zhang
authors.forename: Fu
authors.initials: F
authors.lastname: Cao
authors.forename: Guanjiu
authors.initials: G
authors.lastname: Hu
authors.forename: Yingqun
authors.initials: Y
authors.lastname: Hao
authors.forename: Si
authors.initials: S
authors.lastname: Wei
authors.forename: Xinru
authors.initials: X
authors.lastname: Wang
authors.forename: Hongxia
authors.initials: H
authors.lastname: Yu
mesh_terms: D000818:Animals; D001681:Biological Assay; D002460:Cell Line; D002681:China; D060766:Drinking Water / Q000737:chemistry*; D052244:Endocrine Disruptors / Q000032:analysis / Q000633:toxicity*; D004784:Environmental Monitoring; D011988:Receptors, Thyroid Hormone / Q000037:antagonists & inhibitors* / Q000378:metabolism; D018570:Risk Assessment; D045483:Rivers / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D060766:Drinking Water; D052244:Endocrine Disruptors; D011988:Receptors, Thyroid Hormone; D014874:Water Pollutants, Chemical