title: Critical role of the matricellular protein SPARC in mediating erythroid progenitor cell development in zebrafish.
abstract: Sparc (osteonectin) is a multifunctional matricellular glycoprotein expressed by many differentiated cells. Members of this family mediate cell-matrix interactions rather than acting as structural components of the extracellular matrix (ECM); therefore, they can influence many remodelling events, including haematopoiesis. We have investigated the role of sparc in embryonic haematopoiesis using a morpholino antisense oligonucleotide-based knockdown approach. Knockdown of sparc function resulted in specific erythroid progenitor cell differentiation defects that were highlighted by changes in gene expression and morphology, which could be rescued by injection of sparc mRNA. Furthermore, a comparison of blood phenotypes of sparc and fgfs knockdowns with similar defects and the sparc rescue of the fgf21 blood phenotype places sparc downstream of fgf21 in the genetic network regulating haematopoiesis in zebrafish. These results establish a role for an ECM protein (Sparc) as an important regulator of embryonic haematopoiesis during early development in zebrafish.
authors.affiliation: Aquatic Molecular Pathobiology Laboratory, Instituto Investigaciones Marinas, Consejo Superior de Investigaciones Científicas, Vigo, Spain.
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Ceinos
authors.forename: Eva
authors.initials: E
authors.lastname: Torres-Nuñez
authors.forename: Ruben
authors.initials: R
authors.lastname: Chamorro
authors.forename: Beatriz
authors.initials: B
authors.lastname: Novoa
authors.forename: Antonio
authors.initials: A
authors.lastname: Figueras
authors.forename: Neil M
authors.initials: NM
authors.lastname: Ruane
authors.forename: Josep
authors.initials: J
authors.lastname: Rotllant
mesh_terms: D000818:Animals; D048708:Cell Growth Processes / Q000502:physiology; D002478:Cells, Cultured; D015672:Erythroid Precursor Cells / Q000166:cytology* / Q000378:metabolism*; D015870:Gene Expression; D006410:Hematopoiesis / Q000502:physiology*; D015676:Osteonectin / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015027:Zebrafish
chemical_list: D015676:Osteonectin; D012333:RNA, Messenger