title: A comprehensive study of lysozyme adsorption using dual polarization interferometry and quartz crystal microbalance with dissipation.
abstract: Protein adsorption plays a crucial role in biomaterial surface science as it is directly linked to the biocompatibility of artificial biomaterial devices. Here, elucidation of protein adsorption mechanism is effected using dual polarization interferometry and a quartz crystal microbalance to characterize lysozyme layer properties on a silica surface at different coverage values. Lysozyme is observed to adsorb from sparse monolayer to multilayer coverage. At low coverage an irreversibly adsorbed layer is formed with slight deformation consistent with side-on orientation. At higher coverage values dynamic re-orientation effects are observed which lead to monolayer surface coverages of 2-3 ng/mm² corresponding to edge-on or/and end-on orientations. These monolayer thickness values ranged between 3 and 4.5 nm with a protein density value of 0.60 g/mL and with 50 wt% solvent mass. Further increase of coverage results formation of a multilayer structure. Using the hydration content and other physical layer properties a tentative model lysozyme adsorption is proposed.
authors.affiliation: Nanoscience Centre, Department of Engineering, University of Cambridge, Cambridge CB3 0FF, UK.
authors.forename: Kairuo
authors.initials: K
authors.lastname: Xu
authors.forename: Myriam M
authors.initials: MM
authors.lastname: Ouberai
authors.forename: Mark E
authors.initials: ME
authors.lastname: Welland
mesh_terms: D000327:Adsorption; D004800:Enzymes, Immobilized / Q000032:analysis / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007368:Interferometry / Q000295:instrumentation*; D008422:Materials Testing / Q000295:instrumentation*; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D009113:Muramidase / Q000032:analysis / Q000737:chemistry*; D011485:Protein Binding; D012031:Refractometry / Q000295:instrumentation*
chemical_list: D004800:Enzymes, Immobilized; D009113:Muramidase