title: Ionic liquid-based microwave-assisted extraction of flavonoids from Bauhinia championii (Benth.) Benth.
abstract: An ionic liquids (IL)-based microwave-assisted approach for extraction and determination of flavonoids from Bauhinia championii (Benth.) Benth. was proposed for the first time. Several ILs with different cations and anions and the microwave-assisted extraction (MAE) conditions, including sample particle size, extraction time and  liquid-solid ratio, were investigated. Two M 1-butyl-3-methylimidazolium bromide ([bmim] Br) solution with 0.80 M HCl was selected as the optimal solvent. Meanwhile the optimized conditions a ratio of liquid to material of 30:1, and the extraction for 10 min at 70 &#176;C. Compared with conventional heat-reflux extraction (CHRE) and the regular MAE, IL-MAE exhibited a higher extraction yield and shorter extraction time (from 1.5 h to  10 min). The optimized extraction samples were analysed by LC-MS/MS. IL extracts of Bauhinia championii (Benth.)Benth consisted mainly of flavonoids, among which myricetin, quercetin and kaempferol, &#946;-sitosterol, triacontane and hexacontane were identified. The study indicated that IL-MAE was an efficient and rapid method with simple sample preparation. LC-MS/MS was also used to determine the chemical composition of the ethyl acetate/MAE extract of Bauhinia championii (Benth.) Benth, and it maybe become a rapid method to determine the composition of new plant extracts.
authors.affiliation: Pharmacy College of Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Kedan
authors.initials: K
authors.lastname: Chu
authors.forename: Huang
authors.initials: H
authors.lastname: Li
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Haiyin
authors.initials: H
authors.lastname: Zheng
authors.forename: Ruilan
authors.initials: R
authors.lastname: Chen
authors.forename: Lidian
authors.initials: L
authors.lastname: Chen
mesh_terms: D036481:Bauhinia / Q000737:chemistry*; D002853:Chromatography, Liquid; D005419:Flavonoids / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification; D052578:Ionic Liquids / Q000737:chemistry*; D008872:Microwaves; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D053719:Tandem Mass Spectrometry
chemical_list: D005419:Flavonoids; D052578:Ionic Liquids; D010936:Plant Extracts; D012997:Solvents