title: [Exploring the neural diseases using stem cell technology].
abstract: The rapid and efficient induction of neural stem cells (NSCs) from pluripotent stem cells is required for the research of patient-specific iPS cells and regenerative medicine to induce their own neural cells. Here, we induced NSCs from human pluripotent stem cells within 2 weeks and these clonal NSCs were expanded efficiently by their self-renewal ability. Further, we directly induced NSCs from both mouse and human fibroblasts using four reprogramming factors (Oct4, Sox2, Klf4, and cMyc) without the clonal isolation of induced pluripotent stem cells (iPSCs). Since these NSCs rapidly developed into mature gliogenic neural stem cells, we were able to purify these rapidly differentiating NSCs without contamination of differentiation-resistant pluripotent cells. These methods will facilitate high throughput screening of phonotypes appeared in neural cells induced from the somatic cells derived from sporadic neurodegenerative diseases.
authors.affiliation: Keio University, School of Medicine.
authors.forename: Wado
authors.initials: W
authors.lastname: Akamatsu
mesh_terms: D000818:Animals; D060846:Cell Engineering / Q000379:methods*; D006801:Humans; D057026:Induced Pluripotent Stem Cells; D000090062:Kruppel-Like Factor 4; D051379:Mice; D009422:Nervous System Diseases / Q000628:therapy*