title: Importance of membrane selection in the development of immunochromatographic assays for low-molecular weight compounds.
abstract: This study was performed to demonstrate the importance of selecting an appropriate membrane when developing immunochromatographic assays (ICAs) for the sensitive detection of low-molecular weight compounds. Based on our findings, we propose a theoretical basis for selecting such a membrane. When eluting the sample solution for the competitive ICA using colloidal gold label for low-molecular analytes, the degree of binding inhibition is proportional to the collision frequency between the antibody-colloidal gold (Ab-CG) and analyte before Ab-CG binding to the capture antigen and a higher concentration of pesticides around the Ab-CG leads to a greater degree of inhibition. Therefore, we propose that the relative migration speed of the analyte and Ab-CG on the test strip is critically important for selecting a membrane in the development of sensitive competitive ICAs. We developed a novel method to estimate such a relative migration speed. We demonstrated the applicability of this proposal by using it to select an appropriate membrane for the development of an ICA of the pesticide diazinon.
authors.affiliation: Department of Food Science and Nutrition, Kyungpook National University, Daegu 702-701, South Korea.
authors.forename: Ji-Ye
authors.initials: JY
authors.lastname: Lee
authors.forename: Young Ah
authors.initials: YA
authors.lastname: Kim
authors.forename: Mi Yeon
authors.initials: MY
authors.lastname: Kim
authors.forename: Yong Tae
authors.initials: YT
authors.lastname: Lee
authors.forename: Bruce D
authors.initials: BD
authors.lastname: Hammock
authors.forename: Hye-Sung
authors.initials: HS
authors.lastname: Lee
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D002846:Chromatography, Affinity; D017979:Gold Colloid / Q000737:chemistry; D008567:Membranes, Artificial; D008970:Molecular Weight; D010575:Pesticides / Q000032:analysis* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D017979:Gold Colloid; D008567:Membranes, Artificial; D010575:Pesticides