title: Nanostructure-mediated transport of biologics across epithelial tissue: enhancing permeability via nanotopography.
abstract: Herein, we demonstrate that nanotopographical cues can be utilized to enable biologics >66 kDa to be transported across epithelial monolayers. When placed in contact with epithelial monolayers, nanostructured thin films loosen the epithelial barrier and allow for significantly increased transport of FITC-albumin, FITC-IgG, and a model therapeutic, etanercept. Our work highlights the potential to use drug delivery systems which incorporate nanotopography to increase the transport of biologics across epithelial tissue.
authors.affiliation: UC Berkeley & UCSF Graduate Program in Bioengineering, 1700 4th Street, Building QB3 Room 204, UCSF Mission Bay Campus, San Francisco, California 94158, USA.
authors.forename: Kimberly R
authors.initials: KR
authors.lastname: Kam
authors.forename: Laura A
authors.initials: LA
authors.lastname: Walsh
authors.forename: Suzanne M
authors.initials: SM
authors.lastname: Bock
authors.forename: Michael
authors.initials: M
authors.lastname: Koval
authors.forename: Kathleen E
authors.initials: KE
authors.lastname: Fischer
authors.forename: Russell F
authors.initials: RF
authors.lastname: Ross
authors.forename: Tejal A
authors.initials: TA
authors.lastname: Desai
mesh_terms: D000418:Albumins / Q000493:pharmacokinetics; D001688:Biological Products / Q000493:pharmacokinetics*; D004848:Epithelium / Q000378:metabolism*; D007074:Immunoglobulin G / Q000378:metabolism; D049329:Nanostructures; D010539:Permeability
chemical_list: D000418:Albumins; D001688:Biological Products; D007074:Immunoglobulin G