title: A natural antioxidant pine bark extract, Oligopin®, regulates the stress chaperone HSPB1 in human skeletal muscle cells: a proteomics approach.
abstract: The gradual loss of muscle mass affecting all the elderly (sarcopenia) is most likely due to a decreased number and/or function of satellite cells. Accumulation of reactive oxygen species (ROS) has been clearly correlated to sarcopenia and could contribute to the impairment of satellite cell function. In this study, we analyzed the protective mechanism of action of a natural pine bark extract (Oligopin®) in human muscle satellite cells exposed to oxidative stress (H2O2). This polyphenol belongs to the flavonoid family and was able to abolish the H2 O2-induced apoptotic cell death. A large-scale proteomic strategy allowed us to identify several proteins that may function as early regulators of ROS-mediated events in muscle cells. Interestingly, we identified the stress chaperone heat shock protein beta-1, a main protector of muscle necrosis, as a target of Oligopin® and showed that this polyphenol was able to modulate its stress induced phosphorylation.
authors.affiliation: Univ. Bordeaux, CNRS, CBMN, UMR 5248, F-33600, Pessac, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Poussard
authors.forename: Amélie
authors.initials: A
authors.lastname: Pires-Alves
authors.forename: Ramata
authors.initials: R
authors.lastname: Diallo
authors.forename: Jean-William
authors.initials: JW
authors.lastname: Dupuy
authors.forename: Elise
authors.initials: E
authors.lastname: Dargelos
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism*; D006360:Heat-Shock Proteins; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D018832:Molecular Chaperones; D018384:Oxidative Stress / Q000187:drug effects; D010766:Phosphorylation; D028223:Pinus / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology; D040901:Proteomics; D055948:Sarcopenia; D032496:Satellite Cells, Skeletal Muscle / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; C587735:Oligopin; D010936:Plant Extracts; D059808:Polyphenols; D006861:Hydrogen Peroxide