title: Bicarbonate modulates oxidative and functional damage in ischemia-reperfusion.
abstract: The carbon dioxide/bicarbonate (CO(2)/HCO(3)(-)) pair is the main biological pH buffer. However, its influence on biological processes, and in particular redox processes, is still poorly explored. Here we study the effect of CO(2)/HCO(3)(-) on ischemic injury in three distinct models (cardiac HL-1 cells, perfused rat heart, and Caenorhabditis elegans). We found that, although various concentrations of CO(2)/HCO(3)(-) do not affect function under basal conditions, ischemia-reperfusion or similar insults in the presence of higher CO(2)/HCO(3)(-) resulted in greater functional loss associated with higher oxidative damage in all models. Because the effect of CO(2)/HCO(3)(-) was observed in all models tested, we believe this buffer is an important determinant of oxidative damage after ischemia-reperfusion.
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, 05508-900 São Paulo, SP, Brazil.
authors.forename: Bruno B
authors.initials: BB
authors.lastname: Queliconi
authors.forename: Thire B M
authors.initials: TB
authors.lastname: Marazzi
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Vaz
authors.forename: Paul S
authors.initials: PS
authors.lastname: Brookes
authors.forename: Keith
authors.initials: K
authors.lastname: Nehrke
authors.forename: Ohara
authors.initials: O
authors.lastname: Augusto
authors.forename: Alicia J
authors.initials: AJ
authors.lastname: Kowaltowski
mesh_terms: D000818:Animals; D001639:Bicarbonates / Q000378:metabolism* / Q000494:pharmacology; D002021:Buffers; D017173:Caenorhabditis elegans / Q000187:drug effects / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004195:Disease Models, Animal; D006321:Heart; D006863:Hydrogen-Ion Concentration; D008297:Male; D051379:Mice; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology
chemical_list: D001639:Bicarbonates; D002021:Buffers; D002245:Carbon Dioxide