title: The production of mammalian trematode infective stages by the snail Galba truncatula.
abstract: Several experiments on the breeding of trematode-infected Galba truncatula for obtaining and packaging Fasciola hepatica and Paramphistomum daubneyi metacercariae were carried out to determine the more convenient methods to use for commercial production of these infective stages. Compared to the breeding of infected snails in aquaterraria, the use of 14-cm Petri dishes allowed a greater prevalence of snail infection and a higher number of metacercariae. The production of these larvae was still 2.3-3.4 times greater if infected snails were dissected during the patent period. The aspiration of these metacercariae at the extremity of a Pasteur pipette significantly shortens the time necessary for their transfer from Petri dishes to Eppendorf tubes. Using 14-cm Petri dishes, snail dissection and metacercarial aspiration for their transfer strongly reduce the cost price for metacercarial production of the trematodes Fasciola hepatica and Paramphistomum daubneyi.
authors.affiliation: INSERM U 1094, Faculties of Medicine and Pharmacy, 87025 Limoges, France.
authors.forename: D
authors.initials: D
authors.lastname: Rondelaud
authors.affiliation: INSERM U 1094, Faculties of Medicine and Pharmacy, 87025 Limoges, France.
authors.forename: R
authors.initials: R
authors.lastname: Mouzet
authors.affiliation: INSERM U 1094, Faculties of Medicine and Pharmacy, 87025 Limoges, France.
authors.forename: P
authors.initials: P
authors.lastname: Vignoles
authors.affiliation: INSERM U 1094, Faculties of Medicine and Pharmacy, 87025 Limoges, France.
authors.forename: G
authors.initials: G
authors.lastname: Dreyfuss
authors.affiliation: UMR 1282 Infectiology and Public Health, INRA, 37380 Nouzilly, France.
authors.forename: J
authors.initials: J
authors.lastname: Cabaret
mesh_terms: D027901:Acanthaceae / Q000469:parasitology*; D000818:Animals; D005210:Fasciola hepatica / Q000254:growth & development* / Q000302:isolation & purification*; D010250:Paramphistomatidae / Q000254:growth & development* / Q000302:isolation & purification*; D010274:Parasitology / Q000379:methods*