title: Antibody-functionalized porous silicon nanoparticles for vectorization of hydrophobic drugs.
abstract: We describe the preparation of biodegradable porous silicon nanoparticles (pSiNP) functionalized with cancer cell targeting antibodies and loaded with the hydrophobic anti-cancer drug camptothecin. Orientated immobilization of the antibody on the pSiNP is achieved using novel semicarbazide based bioconjugate chemistry. To demonstrate the generality of this targeting approach, the three antibodies MLR2, mAb528 and Rituximab are used, which target neuroblastoma, glioblastoma and B lymphoma cells, respectively. Successful targeting is demonstrated by means of flow cytometry and immunocytochemistry both with cell lines and primary cells. Cell viability assays after incubation with pSiNPs show selective killing of cells expressing the receptor corresponding to the antibody attached on the pSiNP.
authors.affiliation: Institut Charles Gerhardt Montpellier, UMR 5253 CNRS-ENSCM-UM2-UM1, Ecole Nationale Supérieure de Chimie de Montpellier, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Secret
authors.forename: Kevin
authors.initials: K
authors.lastname: Smith
authors.forename: Valentina
authors.initials: V
authors.lastname: Dubljevic
authors.forename: Eli
authors.initials: E
authors.lastname: Moore
authors.forename: Peter
authors.initials: P
authors.lastname: Macardle
authors.forename: Bahman
authors.initials: B
authors.lastname: Delalat
authors.forename: Mary-Louise
authors.initials: ML
authors.lastname: Rogers
authors.forename: Terrance G
authors.initials: TG
authors.lastname: Johns
authors.forename: Jean-Olivier
authors.initials: JO
authors.lastname: Durand
authors.forename: Frédérique
authors.initials: F
authors.lastname: Cunin
authors.forename: Nicolas H
authors.initials: NH
authors.lastname: Voelcker
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000737:chemistry; D002166:Camptothecin / Q000008:administration & dosage* / Q000737:chemistry; D045744:Cell Line, Tumor; D004058:Diffusion; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008422:Materials Testing; D053769:Nanocapsules / Q000008:administration & dosage* / Q000737:chemistry*; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D016062:Porosity; D012825:Silicon / Q000737:chemistry*; D016896:Treatment Outcome
chemical_list: D000911:Antibodies, Monoclonal; D000972:Antineoplastic Agents, Phytogenic; D053769:Nanocapsules; D002166:Camptothecin; D012825:Silicon