title: The role of biodegradable engineered nanofiber scaffolds seeded with hair follicle stem cells for tissue engineering.
abstract: The aim of this study was to fabricate the poly caprolactone (PCL) aligned nanofiber scaffold and to evaluate the survival, adhesion, proliferation, and differentiation of rat hair follicle stem cells (HFSC) in the graft material using electrospun PCL nanofiber scaffold for tissue engineering applications.
  
  
  
  The bulge region of rat whisker was isolated and cultured in DMEM: nutrient mixture F-12 supplemented with epidermal growth factor. The morphological and biological features of cultured bulge cells were observed by light microscopy using immunocytochemistry methods. Electrospinning was used for production of PCL nanofiber scaffolds. Scanning electron microscopy (SEM), 3-(4, 5-di-methylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, and histology analysis were used to investigate the cell morphology, viability, attachment and infiltration of the HFSC on the PCL nanofiber scaffolds.
  
  
  
  The results of the MTT assay showed cell viability and cell proliferation of the HFSC on PCL nanofiber scaffolds. SEM microscopy images indicated that HFSC are attached, proliferated and spread on PCL nanofiber scaffolds. Also, immunocytochemical analysis showed cell infiltration and cell differentiation on the scaffolds.
  
  
  
  The results of this study reveal that PCL nanofiber scaffolds are suitable for cell culture, proliferation, differentiation and attachment. Furthermore, HFSC are attached and proliferated on PCL nanofiber scaffolds.
authors.affiliation: Dept. of Histology and Neuroscience, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.|Dept. of Anatomy, School of Medicine, Babol University of Medical Science, Babol, Iran.
authors.forename: Leila Beigom
authors.initials: LB
authors.lastname: Hejazian
authors.affiliation: Dept. of Anatomy, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran.
authors.forename: Banafshe
authors.initials: B
authors.lastname: Esmaeilzade
authors.affiliation: Dept. of Histology and Neuroscience, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Fatima
authors.initials: F
authors.lastname: Moghanni Ghoroghi
authors.affiliation: Dept. of Histology and Neuroscience, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Moradi
authors.affiliation: Physical Education and Sports Sciences, Dept. of Physical Education, Payame Noor University of Tehran, Tehran, Iran.
authors.forename: Marzieh Beigom
authors.initials: MB
authors.lastname: Hejazian
authors.affiliation: Microscopic Surgen, Minimally Invasive Surgery Research Center, Rassoul Akram Hospital, Tehran University of Medical Science, Tehran, Iran.
authors.forename: Anahita
authors.initials: A
authors.lastname: Aslani
authors.affiliation: Dept. of Histology and Neuroscience, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Bakhtiari
authors.affiliation: 6Dept. of Hematology, School of Medicine, Tarbiat Modares University, Tehran, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.affiliation: Anti-microbial Resistance Research Center, Tehran University of Medical Science, Tehran, Iran.
authors.forename: Maliheh
authors.initials: M
authors.lastname: Nobakht
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D018859:Hair Follicle / Q000166:cytology*; D008297:Male; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000648:ultrastructure; D011091:Polyesters; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D011091:Polyesters; C016240:polycaprolactone