title: Interactions of the antitumor macrolide aplyronine A with actin and actin-related proteins established by its versatile photoaffinity derivatives.
abstract: The antitumor and apoptogenic macrolide aplyronine A (ApA) is a potent actin-depolymerizing agent. We developed an ApA acetylene analog that bears the aryldiazirine group at the C34 terminus, which formed a covalent bond with actin. With the use of the photoaffinity biotin derivatives of aplyronines A and C, Arp2 and Arp3 (actin-related proteins) were specifically purified as binding proteins along with actin from tumor cell lysate. However, Arp2 and Arp3 did not covalently bind to aplyronine photoaffinity derivatives. Thus, actin-related proteins might indirectly bind to ApA as the ternary adducts of the actin/ApA complex or through the oligomeric actin.
authors.affiliation: Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, 305-8571, Japan. ita@chem.tsukuba.ac.jp
authors.forename: Masaki
authors.initials: M
authors.lastname: Kita
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Hirayama
authors.forename: Kota
authors.initials: K
authors.lastname: Yamagishi
authors.forename: Kozo
authors.initials: K
authors.lastname: Yoneda
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Fujisawa
authors.forename: Hideo
authors.initials: H
authors.lastname: Kigoshi
mesh_terms: D000199:Actins / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D018942:Macrolides / Q000737:chemistry*; D019920:Photoaffinity Labels; D011506:Proteins / Q000737:chemistry*
chemical_list: D000199:Actins; D000970:Antineoplastic Agents; D018942:Macrolides; D019920:Photoaffinity Labels; D011506:Proteins; C104057:aplyronine A