title: Correlation between cell attachment areas after 2 h of culture and osteogenic differentiation activity of rat mesenchymal stem cells on hydroxyapatite substrates with various surface properties.
abstract: The initial attachment of mesenchymal stem cells (MSCs) to substrates and osteogenic differentiation are supported by culture on a hydroxyapatite substrate. Cell attachment areas of rat MSCs after 2 h of culture on hydroxyapatite substrates with various microstructures and the osteogenic differentiation activity thereafter were measured. The perceived outcome was that, after 2 h of culture, rat MSCs with a small attachment area would have a high osteogenic differentiation activity, whereas those with a large attachment area would have a low osteogenic differentiation activity. Furthermore, rat MSCs with a small attachment area had many cytoplasmic processes, while those with a large attachment area revealed clear stress fibers and focal contacts. These results suggest that cell attachment area of rat MSCs after 2 h of culture has a strong effect on the osteogenic differentiation of rat MSCs. Thus, the measurement of cell attachment area after 2 h of culture could become valuable for estimating the osteogenic differentiation activity of rat MSCs thereafter.
authors.affiliation: Faculty of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.
authors.forename: Kan
authors.initials: K
authors.lastname: Cheng
authors.forename: Motohiro
authors.initials: M
authors.lastname: Hirose
authors.forename: Xiupeng
authors.initials: X
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Sogo
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamazaki
authors.forename: Atsuo
authors.initials: A
authors.lastname: Ito
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000648:ultrastructure; D017886:Durapatite / Q000737:chemistry*; D005453:Fluorescence; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology* / Q000648:ultrastructure; D010012:Osteogenesis; D051381:Rats; D013499:Surface Properties; D013997:Time Factors
chemical_list: D017886:Durapatite