title: Changes on the Caco-2 secretome through differentiation analyzed by 2-D differential in-gel electrophoresis (DIGE).
abstract: Colorectal cancer is still a major health burden worldwide, and its diagnosis has not improved in recent years due to a lack of appropriate diagnostic serum markers. Aiming to find new diagnostic proteins, we applied the proteomic DIGE technology to analyze changes in the secretome before/after differentiation of the colon adenocarcinoma Caco-2 cell line, an accepted in vitro model to study colorectal tumorigenesis. When the secretomes from undifferentiated (tumor-like) and differentiated cells (resembling healthy enterocytes) were compared, we found 96 spots differentially expressed. After MS/MS analysis, 22 spots corresponding to 15 different proteins were identified. Principal component analysis demonstrated these 22 spots could serve as a discriminatory panel between the tumor-like and normal-like cells. Among the identified proteins, the translationally-controlled tumor protein (TCTP), the transforming growth factor-beta-induced protein ig-h3 (TGFβIp), and the Niemann-Pick disease type C2 protein (NPC2) are interesting candidates for future studies focused on their utility as serum biomarkers of colorectal cancer.
authors.affiliation: Department of Biochemistry, Genetics and Immunology, Faculty of Biology, University of Vigo, 36310 Vigo, Spain. vzorzano@uvigo.es.
authors.forename: Andrés
authors.initials: A
authors.lastname: García-Lorenzo
authors.forename: Ana M
authors.initials: AM
authors.lastname: Rodríguez-Piñeiro
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Rodríguez-Berrocal
authors.forename: María Páez de la
authors.initials: MP
authors.lastname: Cadena
authors.forename: Vicenta S
authors.initials: VS
authors.lastname: Martínez-Zorzano
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D018938:Caco-2 Cells; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology; D006801:Humans; D060787:Neoplasm Grading; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D000090324:Tumor Protein, Translationally-Controlled 1; D057825:Two-Dimensional Difference Gel Electrophoresis
chemical_list: D015415:Biomarkers; D020543:Proteome; C000715647:TPT1 protein, human; D000090324:Tumor Protein, Translationally-Controlled 1