title: Protective effect of 70% ethanolic extract of Lindera obtusiloba Blume on tert-butyl hydroperoxide-induced oxidative hepatotoxicity in rats.
abstract: Lindera obtusiloba Blume, a native plant of East Asia, has traditionally been used as a folk medicine for liver disease. We studied the in vitro antioxidant and in vivo hepatoprotective activities of a 70% ethanolic extract of L. obtusiloba (LOE) containing 62.9% quercitrin and 22.0% afzelin. LOE prevented tert-butyl hydroperoxide (t-BHP)-induced oxidative damage in HepG2 cells. Along with its high antioxidant potency in vitro, our animal study confirmed that pretreatment with LOE (500 or 2000 mg/kg) for 7 days prior to a single dose of t-BHP (i.p.: 0.5 mmol/kg) significantly lowered the serum levels of alanine and aspartate aminotransferases. In addition, glutathione levels were increased in the liver, and lipid peroxidation levels were decreased in a dose-dependent manner. The histopathological examinations of rat livers showed that LOE significantly reduced the incidence of liver lesions induced by t-BHP. Therefore, we concluded that LOE has merit as a potent candidate to protect the liver against oxidative damage.
authors.affiliation: Division of Food Bioscience & Technology, College of Life Sciences and Biotechnology, Korea University, 1, 5-ga, Anam-dong, Sungbuk-ku, Seoul 136-701, South Korea.
authors.forename: Chung-Oui
authors.initials: CO
authors.lastname: Hong
authors.forename: Chae Hong
authors.initials: CH
authors.lastname: Rhee
authors.forename: Nam-Hee
authors.initials: NH
authors.lastname: Won
authors.forename: Hee-Don
authors.initials: HD
authors.lastname: Choi
authors.forename: Kwang-Won
authors.initials: KW
authors.lastname: Lee
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001219:Aspartate Aminotransferases / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D000431:Ethanol; D005978:Glutathione / Q000032:analysis / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D027443:Lindera / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols / Q000032:analysis / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D020122:tert-Butylhydroperoxide / Q000009:adverse effects
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D059808:Polyphenols; D000431:Ethanol; D020122:tert-Butylhydroperoxide; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione