title: Phase diagrams map the properties of antiviral agents directed against hepatitis B virus core assembly.
abstract: Assembly effectors are small molecules that induce inappropriate virus capsid assembly to antiviral effect. To identify attributes of hepatitis B virus (HBV) assembly effectors, assembly reaction products (normal capsid, noncapsid polymer, intermediates, and free dimeric core protein) were quantified in the presence of three experimental effectors: HAP12, HAP13, and AT-130. Effectors bound stoichiometrically to capsid protein polymers, but not free protein. Thermodynamic and kinetic effects, not aberrant assembly, correlate with maximal antiviral activity.
authors.affiliation: Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN, USA.
authors.forename: Lichun
authors.initials: L
authors.lastname: Li
authors.forename: Srinivas Reddy
authors.initials: SR
authors.lastname: Chirapu
authors.forename: M G
authors.initials: MG
authors.lastname: Finn
authors.forename: Adam
authors.initials: A
authors.lastname: Zlotnick
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry*; D001549:Benzamides / Q000737:chemistry*; D001665:Binding Sites; D002213:Capsid / Q000737:chemistry*; D036022:Capsid Proteins / Q000737:chemistry*; D006515:Hepatitis B virus / Q000737:chemistry*; D007700:Kinetics; D008958:Models, Molecular; D011485:Protein Binding; D055503:Protein Multimerization; D011743:Pyrimidines / Q000737:chemistry*; D013816:Thermodynamics; D014758:Viral Core Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry*; D019065:Virus Assembly
chemical_list: C471028:AT 130; D000998:Antiviral Agents; D001549:Benzamides; D036022:Capsid Proteins; D011743:Pyrimidines; D014758:Viral Core Proteins