title: Aberration compensation for objective phase curvature in phase holographic microscopy.
abstract: In this study, we proposed a simple and fast numerical approach to compensate for aberrations induced by objective phase curvature. This method is based on the extraction of virtual background phase from reconstructed phase values using a line profile, followed by subtraction of the virtual background phase from the reconstructed phase image. The performance and feasibility of the method were demonstrated by applying it to the phase imaging of polystyrene microspheres and red blood cells.
authors.affiliation: Center for Biofluid and Biomimic Research, Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang, South Korea.
authors.forename: Kyung Won
authors.initials: KW
authors.lastname: Seo
authors.forename: Yong Seok
authors.initials: YS
authors.lastname: Choi
authors.forename: Eun Seok
authors.initials: ES
authors.lastname: Seo
authors.forename: Sang Joon
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D016477:Artifacts; D004912:Erythrocytes / Q000166:cytology; D006696:Holography / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007909:Lenses; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D008863:Microspheres; D055620:Optical Phenomena; D011137:Polystyrenes / Q000737:chemistry; D013997:Time Factors
chemical_list: D011137:Polystyrenes