title: Branched DNA nanostructures efficiently stabilised and monitored by novel pyrene-perylene 2'-α-L-amino-LNA FRET pairs.
abstract: Novel pyrene-perylene α-L-LNA FRET pairs described herein effectively detect assembly of 2- and 3-way branched DNA nanostructures prepared by postsynthetic microwave-assisted CuAAC click chemistry. The fluorescent signalling of assembly by internally positioned FRET pairs is achieved with low to no fluorescence background signal, remarkably low limit of target detection values and stabilization of the resulting nanostructures.
authors.affiliation: Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, DK-5230 Odense M, Denmark. ias@sdu.dk
authors.forename: I Kira
authors.initials: IK
authors.lastname: Astakhova
authors.forename: T Santhosh
authors.initials: TS
authors.lastname: Kumar
authors.forename: Meghan A
authors.initials: MA
authors.lastname: Campbell
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Ustinov
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Korshun
authors.forename: Jesper
authors.initials: J
authors.lastname: Wengel
mesh_terms: D057930:Click Chemistry; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D008872:Microwaves; D049329:Nanostructures / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; D011721:Pyrenes; C477371:locked nucleic acid; D004247:DNA; C030984:pyrene