title: [Determination of nine steroidal saponins in Paris polyphylla from different areas of Guizhou province by HPLC].
abstract: OBJECTIVE
  To compare steroidal saponins-main active ingredients of Paris polyphylla from different areas in Guizhou province, in order to provided basis for further proving Guizhou province to be the planting base of P. polyphylla.
  
  
  METHODS
  The contents of nine steroidal saponins, namely paris-VII, (25R)-5-en-spirost-3beta,17alpha-diol-3-O-alpha-L-rhamnopyranosyl-(1-->2) [alpha-L-rhamnopyranosyl(1-->4)]-beta-D-glycopyanoside (PGRR), paris-H, paris-VI, paris-II , paris-III, gracillin, paris-I and paris-V of P. polyphylla from eight areas of Guizhou province were determined by HPLC-UV.
  
  
  RESULTS
  Five steroidal saponins-paris-VII, PGRR, paris-H, paris-VI and gracillin were detected in all the drugs, which provided basis for distinguishing P. polyphylla. P. polyphylla from Dushan showed the highest content of steroidal saponins (9.62%), followed by Tongren (6.39%), and the lowest was Zunyi (0.99%).
  
  
  CONCLUSIONS
  Saponins from different areas of Guizhou province show significant difference in variety and content, therefore Dushan is suitable for planting P. polyphylla.
authors.affiliation: Tongren Polytechnic, Tongren 554300, China.
authors.forename: Yuyong
authors.initials: Y
authors.lastname: Liang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Gao
authors.forename: Shuli
authors.initials: S
authors.lastname: Man
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019668:Liliaceae / Q000737:chemistry*; D010840:Phytosterols / Q000032:analysis* / Q000302:isolation & purification; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification; D027343:Rhizome / Q000737:chemistry*; D012503:Saponins / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D010840:Phytosterols; D010936:Plant Extracts; D012503:Saponins