title: Genotoxic and antigenotoxic activity of acerola (Malpighia glabra L.) extract in relation to the geographic origin.
abstract: Malpighia glabra L, popularly known as acerola, is considered a functional fruit and therefore is taken to prevent disease or as adjuvant to treatment strategies, since the fruit is an undeniable source of vitamin C, carotenoids, and flavonoids. Acerola is a natural source of vitamin C, flavonoids, and carotenoids. Its chemical composition is affected by genetic uniformity of the orchards and environmental factors. Considering the extensive growth of the culture of acerola in Brazil as well as its widespread use, this study evaluates the genotoxic and antigenotoxic activity of acerola in relation to geographical origin using the comet assay in mice blood cells in vitro. No acerola samples showed potential to induce DNA damage, independently of origin. Also, for antigenotoxicity activity, only the acerola sample from São Paulo reduced DNA damage induced by hydrogen peroxide (by about 56%). The sample from Ceará showed good antioxidant activity by the 2,2-diphenyl-1-picrylhydrazyl assay, in agreement with its higher rutin, quercetin, and vitamin C levels. Additional studies with other treatment regimens are necessary to better understand the impact of the complex mixture of acerola on genomic stability.
authors.affiliation: Laboratory of Toxicologic Genetics, Post-graduation in Cellular and Molecular Biology Applied to Health (PPGBioSaúde), Lutheran Univ. Brazil, ULBRA, 92.425-900, Canoas, RS, Brazil; Health Science Center, Region of Campanha University, URCAMP, 96.400-110, Bagé, RS, Brazil.
authors.forename: Roberta Da Silva
authors.initials: Rda S
authors.lastname: Nunes
authors.forename: Vivian Francília Silva
authors.initials: VF
authors.lastname: Kahl
authors.forename: Merielen Da Silva
authors.initials: Mda S
authors.lastname: Sarmento
authors.forename: Marc François
authors.initials: MF
authors.lastname: Richter
authors.forename: Juan Andres
authors.initials: JA
authors.lastname: Abin-Carriquiry
authors.forename: Marcela María
authors.initials: MM
authors.lastname: Martinez
authors.forename: Alexandre De Barros Falcão
authors.initials: Ade B
authors.lastname: Ferraz
authors.forename: Juliana
authors.initials: J
authors.lastname: Da Silva
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis; D001713:Biphenyl Compounds; D001938:Brazil; D002851:Chromatography, High Pressure Liquid; D020552:Comet Assay; D004249:DNA Damage; D016166:Free Radical Scavengers / Q000737:chemistry; D005609:Free Radicals; D005638:Fruit / Q000737:chemistry; D005843:Geography; D020128:Inhibitory Concentration 50; D008297:Male; D029581:Malpighiaceae / Q000737:chemistry*; D051379:Mice; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry*; D011794:Quercetin / Q000032:analysis; D012431:Rutin / Q000032:analysis
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D010851:Picrates; D010936:Plant Extracts; D012431:Rutin; D011794:Quercetin; C004931:1,1-diphenyl-2-picrylhydrazyl; D001205:Ascorbic Acid