title: Sharka: the past, the present and the future.
abstract: Members the Potyviridae family belong to a group of plant viruses that are causing devastating plant diseases with a significant impact on agronomy and economics. Plum pox virus (PPV), as a causative agent of sharka disease, is widely discussed. The understanding of the molecular biology of potyviruses including PPV and the function of individual proteins as products of genome expression are quite necessary for the proposal the new antiviral strategies. This review brings to view the members of Potyviridae family with respect to plum pox virus. The genome of potyviruses is discussed with respect to protein products of its expression and their function. Plum pox virus distribution, genome organization, transmission and biochemical changes in infected plants are introduced. In addition, techniques used in PPV detection are accentuated and discussed, especially with respect to new modern techniques of nucleic acids isolation, based on the nanotechnological approach. Finally, perspectives on the future of possibilities for nanotechnology application in PPV determination/identification are outlined.
authors.affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. sochor.jirik@seznam.cz
authors.forename: Jiri
authors.initials: J
authors.lastname: Sochor
authors.forename: Petr
authors.initials: P
authors.lastname: Babula
authors.forename: Vojtech
authors.initials: V
authors.lastname: Adam
authors.forename: Boris
authors.initials: B
authors.lastname: Krska
authors.forename: Rene
authors.initials: R
authors.lastname: Kizek
mesh_terms: D016679:Genome, Viral; D010935:Plant Diseases / Q000821:virology*; D017801:Plum Pox Virus / Q000502:physiology*