title: Suppression of Penaeus merguiensis densovirus following oral delivery of live bacteria expressing dsRNA in the house cricket (Acheta domesticus) model.
abstract: Penaeus merguiensis densovirus (PmergDNV) is a serious pathogen of the banana prawn, Penaeus merguiensis leading to at least 28% production loss due to reduced growth rates and mortality of juveniles. In the present study, we reduced PmergDNV titres and subsequent mortality by feeding Acheta domesticus (previously determined as an appropriate animal model for P. merguiensis) with dsRNA specific to the capsid protein by mixing it into their food. Feeding A. domesticus with PmergDNV-specific dsRNA in advance of viral challenge increased their longevity, decreased mortality by 84.4% and reduced viral loads 24-fold below the threshold level required for mortality. Mortalities and viral loads were significantly (both P < 0.001) lower in treatments challenged with PmergDNV following exposure to bacterially expressed PmergDNV-dsRNA. This is the first study to demonstrate gene silencing via RNAi against PmergDNV in vivo through oral administration of live bacteria expressing dsRNA in a model system.
authors.affiliation: School of Veterinary and Biomedical Sciences, James Cook University, Townsville, QLD 4811, Australia. Kathy.lafauce1@jcu.edu.au
authors.forename: Kathy
authors.initials: K
authors.lastname: La Fauce
authors.forename: Leigh
authors.initials: L
authors.lastname: Owens
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics*; D060085:Coinfection; D017990:Densovirus / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D020868:Gene Silencing; D006135:Gryllidae / Q000821:virology*; D007304:Insect Viruses; D007306:Insecticides; D008136:Longevity / Q000187:drug effects; D033561:Penaeidae / Q000821:virology*; D010572:Pest Control, Biological / Q000379:methods; D034622:RNA Interference; D012329:RNA, Bacterial / Q000008:administration & dosage* / Q000235:genetics; D012330:RNA, Double-Stranded / Q000008:administration & dosage* / Q000235:genetics; D019562:Viral Load
chemical_list: D007306:Insecticides; D012329:RNA, Bacterial; D012330:RNA, Double-Stranded