title: Effect of long-term starvation in salty microcosm on biofilm formation and motility in Pseudomonas aeruginosa.
abstract: The development of antibiotic resistance in the opportunistic pathogen Pseudomonas aeruginosa is a major cause of the pathogen's morbidity and is strongly correlated with the biofilm formation. Motility and adherence capacity in long-term stressed cells have not been extensively analyzed even though P. aeruginosa considered a model organism for the study of biofilm formation. In this investigation, P. aeruginosa ATCC 27853 strain has been stored for 12 months in LB broth with 0.5 M NaCl. Several experiments demonstrated that the strain recovery from the salty microcosm had the ability to increase the biofilm formation and to reduce motility comparing with that of the original strain. To identify genes involved in the regulation of biofilm and/or in stress response by the recovered P. aeruginosa, differential display "DDRT-PCR" technique was used. The genes speD and ccoN2, coding, respectively, for an S-adenosylmethionine decarboxylase and Cbb3-type cytochrome oxidase, were identified in recovered strain of P. aeruginosa ATCC 27853 as two differentially expressed gene fragments. A comparison of the biofilm produced by the wild-type strain PA14 and the transposon insertion mutant for speD gene suggested that spermidine has a potential role in the adaptive response in P. aeruginosa incubated in long-term stress conditions.
authors.affiliation: Laboratory of Analysis, Treatment and Valorization of Environment Polluants and Products, Faculty of Pharmacy, University of Monastir, 5000 Monastir, Tunisia. elabedhamouda@yahoo.fr
authors.forename: Hamouda
authors.initials: H
authors.lastname: Elabed
authors.forename: Makaoui
authors.initials: M
authors.lastname: Maatallah
authors.forename: Rim
authors.initials: R
authors.lastname: Hamza
authors.forename: Ibtissem
authors.initials: I
authors.lastname: Chakroun
authors.forename: Amina
authors.initials: A
authors.lastname: Bakhrouf
authors.forename: Kamel
authors.initials: K
authors.lastname: Gaddour
mesh_terms: D012437:Adenosylmethionine Decarboxylase / Q000096:biosynthesis; D001422:Bacterial Adhesion; D018441:Biofilms / Q000254:growth & development*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000096:biosynthesis; D020869:Gene Expression Profiling; D008124:Locomotion; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D012965:Sodium Chloride; D003576:Electron Transport Complex IV; D012437:Adenosylmethionine Decarboxylase