title: Application of DNA sequence analysis based on five different conserved genes (16S rDNA, rpoB, gdh, est and pgm) for intra-species discrimination of Bacteroides fragilis.
abstract: In the past few years, many studies revealed a remarkable genetic variability in Bacteroides fragilis species, and the existence of two divisions was proposed according to presence or absence of the cfiA (metallo-β-lactamase/carbapenemase) gene. The aim of this study was to evaluate the use of DNA sequence analysis for glutamate dehydrogenase (gdh), phosphoglucomutase (pgm) and esterase (est) metabolic genes, in comparison to RNA polymerase β subunit (rpoB) and 16S ribosomal RNA (rrs) gene sequencing, to identify the presence of these two groups in seventeen B. fragilis strains. Based on phylogenetic trees, only the est gene sequences generated a classification similar to rrs- and rpoB-genes. On the other hand, the genes pgm and gdh did not allow the discrimination of these divisions. The est gene sequence can be suggested as an additional tool for differentiation of the two groups in B. fragilis, providing highly reproducible and reliable data in B. fragilis taxonomy.
authors.affiliation: Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Brazil. karlarodr@yahoo.com.br
authors.forename: Karla Rodrigues
authors.initials: KR
authors.lastname: Miranda
authors.forename: Felipe Piedade Gonçalves
authors.initials: FP
authors.lastname: Neves
authors.forename: Joaquim dos
authors.initials: Jd
authors.lastname: Santos-Filho
authors.forename: Geraldo Renato
authors.initials: GR
authors.lastname: de Paula
authors.forename: Leandro Araújo
authors.initials: LA
authors.lastname: Lobo
authors.forename: Walter Martin Roland
authors.initials: WM
authors.lastname: Oelemann
authors.forename: Regina Maria Cavalcanti Pilotto
authors.initials: RM
authors.lastname: Domingues
mesh_terms: D001441:Bacteroides fragilis / Q000145:classification* / Q000235:genetics*; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D058889:Molecular Typing / Q000379:methods*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods*