title: Cytosolic protection against ultraviolet induced DNA damage by blueberry anthocyanins and anthocyanidins in hepatocarcinoma HepG2 cells.
abstract: UV-induced DNA damage plays a key role in the etiology of certain diseases. The ability of blueberry anthocyanins and anthocyanidins (BA) to protect cellular DNA from UV-induced damage was investigated. BA were extracted by water (BAW), ethanol (BAE) or methanol (BAM). These extracts partially restored proliferation of UV-irradiated HepG2 cells as shown by MTT assay. Treatment with BA extracts at 75 μg/ml decreased reactive oxygen species and decreased DNA damage by tail moment of comet assay and expression of γH2AX in situ. BAM significantly decreased gene and protein expression of p53, phospho-p53 (Ser15), and p21 in UV-irradiated HepG2 cells. BA thus efficiently protects cells from DNA damage in vitro. Blueberry may potentially be used as a good source of naturally radioprotective agents.
authors.affiliation: Key Laboratory of Food Nutrition and Safety, School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, No. 29, The 13th Avenue, TEDA, Tianjin 300457, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Xiangyi
authors.initials: X
authors.lastname: Lu
authors.forename: Guangyang
authors.initials: G
authors.lastname: He
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.forename: Mengxian
authors.initials: M
authors.lastname: Li
authors.forename: Junhua
authors.initials: J
authors.lastname: Wu
authors.forename: Zhenjing
authors.initials: Z
authors.lastname: Li
authors.forename: Jihui
authors.initials: J
authors.lastname: Wu
authors.forename: Juncheng
authors.initials: J
authors.lastname: Wang
authors.forename: Cheng
authors.initials: C
authors.lastname: Luo
mesh_terms: D000872:Anthocyanins / Q000302:isolation & purification / Q000494:pharmacology*; D029798:Blueberry Plants / Q000737:chemistry*; D002470:Cell Survival; D020552:Comet Assay; D004249:DNA Damage / Q000528:radiation effects*; D020869:Gene Expression Profiling; D056945:Hep G2 Cells; D006801:Humans; D011837:Radiation-Protective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000032:analysis; D013194:Staining and Labeling / Q000379:methods; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D000872:Anthocyanins; D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue