title: Quantitative proteomic analysis of ibuprofen-degrading Patulibacter sp. strain I11.
abstract: Ibuprofen is the third most consumed pharmaceutical drug in the world. Several isolates have been shown to degrade ibuprofen, but very little is known about the biochemistry of this process. This study investigates the degradation of ibuprofen by Patulibacter sp. strain I11 by quantitative proteomics using a metabolic labelling strategy. The whole-genome of Patulibacter sp. strain I11 was sequenced to provide a species-specific protein platform for optimal protein identification. The bacterial proteomes of actively ibuprofen-degrading cells and cells grown in the absence of ibuprofen was identified and quantified by gel based shotgun-proteomics. In total 251 unique proteins were quantitated using this approach. Biological process and pathway analysis indicated a number of proteins that were up-regulated in response to active degradation of ibuprofen, some of them are known to be involved in the degradation of aromatic compounds. Data analysis revealed that several of these proteins are likely involved in ibuprofen degradation by Patulibacter sp. strain I11.
authors.affiliation: IBET, Apartado 12, 2781-901, Oeiras, Portugal.
authors.forename: B
authors.initials: B
authors.lastname: Almeida
authors.forename: H
authors.initials: H
authors.lastname: Kjeldal
authors.forename: I
authors.initials: I
authors.lastname: Lolas
authors.forename: A D
authors.initials: AD
authors.lastname: Knudsen
authors.forename: G
authors.initials: G
authors.lastname: Carvalho
authors.forename: K L
authors.initials: KL
authors.lastname: Nielsen
authors.forename: M T
authors.initials: MT
authors.lastname: Barreto Crespo
authors.forename: A
authors.initials: A
authors.lastname: Stensballe
authors.forename: J L
authors.initials: JL
authors.lastname: Nielsen
mesh_terms: D039903:Actinobacteria / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D001673:Biodegradation, Environmental / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D023281:Genomics; D007052:Ibuprofen / Q000378:metabolism* / Q000494:pharmacology; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D012333:RNA, Messenger; D007052:Ibuprofen