title: Enzymatic Synthesis of t,2-di-Sinapoylglucose from 1-Sinapoylglucose by a Protein Preparation from Cotyledons of Raphanus sstivus Grown in the Dark.
abstract: Protein preparation from cotyledons of Raphanus sativus seedlings grown in the dark catalyzed the transfer of the sinapoyl moiety of 1-O-sinapoyl-ß-D-glucose to the C-2 hydroxyl group of the glucose moiety of another molecule of 1-O-sinapcyl-ß-D-glucose to form 1,2-di-O-sinapoyl-ß-D-glucopyranose. In this synthesis the 1-O-acyl glucoside was used both as acyl donor and acceptor molecule. Maximal rate of product formation was found to be at pH 7.5 in 0.1M Hepes buffer.
authors.affiliation: Botanisches Institut der Universität zu Köln, Gyrhofstraße 15, D-5000 Köln 41, Federal Republic of Germany.
authors.forename: B
authors.initials: B
authors.lastname: Dahlbender
authors.forename: D
authors.initials: D
authors.lastname: Strack