title: Extracellular ATP inhibits Schwann cell dedifferentiation and proliferation in an ex vivo model of Wallerian degeneration.
abstract: After nerve injury, Schwann cells proliferate and revert to a phenotype that supports nerve regeneration. This phenotype-changing process can be viewed as Schwann cell dedifferentiation. Here, we investigated the role of extracellular ATP in Schwann cell dedifferentiation and proliferation during Wallerian degeneration. Using several markers of Schwann cell dedifferentiation and proliferation in sciatic explants, we found that extracellular ATP inhibits Schwann cell dedifferentiation and proliferation during Wallerian degeneration. Furthermore, the blockage of lysosomal exocytosis in ATP-treated sciatic explants is sufficient to induce Schwann cell dedifferentiation. Together, these findings suggest that ATP-induced lysosomal exocytosis may be involved in Schwann cell dedifferentiation.
authors.affiliation: Department of Anatomy, College of Medicine, Kyung Hee University, Heogi-Dong 1, Dongdaemun-Gu, Seoul 130-701, Republic of Korea.
authors.forename: Youn Ho
authors.initials: YH
authors.lastname: Shin
authors.forename: Seo Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Junyang
authors.initials: J
authors.lastname: Jung
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D054337:Cell Dedifferentiation; D049109:Cell Proliferation; D005089:Exocytosis; D051907:Lysosomal Membrane Proteins / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D008687:Metformin / Q000494:pharmacology; D008954:Models, Biological; D009419:Nerve Tissue Proteins; D051381:Rats; D017207:Rats, Sprague-Dawley; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor / Q000235:genetics / Q000378:metabolism; D012583:Schwann Cells / Q000378:metabolism / Q000473:pathology*; D020426:Sciatic Neuropathy / Q000378:metabolism / Q000473:pathology; D014855:Wallerian Degeneration / Q000378:metabolism / Q000473:pathology*; D052118:Lysosomal-Associated Membrane Protein 1
chemical_list: D000255:Adenosine Triphosphate; D051907:Lysosomal Membrane Proteins; D008687:Metformin; D009419:Nerve Tissue Proteins; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor; C489117:Lamp1 protein, rat; D052118:Lysosomal-Associated Membrane Protein 1; C068313:Ngfr protein, rat