title: Cerebral blood volume combined with amplitude-integrated EEG can be a suitable guide to control hypoxic/ischemic insult in a piglet model.
abstract: BACKGROUND
  The purposes of this study are to compare two hypoxic/ischemic (H/I) insults using amplitude-integrated EEG (aEEG), alone or combined with cerebral blood volume (CBV), as a guide to control hypoxia and to determine which protocol most effectively produces a consistent degree of survivable neuropathological damage in a newborn piglet model of perinatal asphyxia.
  
  
  METHODS
  Eighteen piglets were subjected to H/I insult of 20-min low aEEG (LAEEG). After the 20-min, the aEEG group was maintained with low mean arterial blood pressure for 10min. The procedure for the aEEG plus CBV group was stopped if CBV became the rated value after 20min of LAEEG. We measured changes in CBV using a near-infrared time-resolved spectroscopy (TRS) and cerebral electrocortical activity using aEEG until 6h post-insult. At 5days post insult, the piglets' brains were perfusion-fixed and stained with hematoxylin/eosin. Piglets were grouped as undamaged or damaged; piglets that did not survive to 5days were grouped separately as dead.
  
  
  RESULTS
  Among surviving piglets, CBV combined with aEEG resulted in significantly greater percentage of damaged piglets than aEEG alone.
  
  
  CONCLUSIONS
  We conclude that combining CBV with aEEG may be a more effective guide to control H/I insult in a newborn piglet model than aEEG alone.
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Nakamura
authors.forename: Takashi
authors.initials: T
authors.lastname: Kusaka
authors.forename: Saneyuki
authors.initials: S
authors.lastname: Yasuda
authors.forename: Masaki
authors.initials: M
authors.lastname: Ueno
authors.forename: Takanori
authors.initials: T
authors.lastname: Miki
authors.forename: Kosuke
authors.initials: K
authors.lastname: Koyano
authors.forename: Makoto
authors.initials: M
authors.lastname: Nakamura
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Okada
authors.forename: Kaoru
authors.initials: K
authors.lastname: Okazaki
authors.forename: Kenichi
authors.initials: K
authors.lastname: Isobe
authors.forename: Susumu
authors.initials: S
authors.lastname: Itoh
mesh_terms: D000818:Animals; D001238:Asphyxia Neonatorum / Q000503:physiopathology; D001921:Brain / Q000098:blood supply*; D002560:Cerebrovascular Circulation; D004195:Disease Models, Animal; D004569:Electroencephalography / Q000379:methods*; D020925:Hypoxia-Ischemia, Brain; D019265:Spectroscopy, Near-Infrared; D013552:Swine