title: A rapid purification procedure for the HsdM protein of EcoR124I and biophysical characterization of the purified protein.
abstract: Type I restriction-modification (R-M) systems are comprised of two multi-subunit enzymes with complementary functions: the methyltransferase (~160 kDa), responsible for methylation of DNA, and the restriction endonuclease (~400 kDa), responsible for DNA cleavage. Both enzymes share a number of subunits, including HsdM. Characterisation of either enzyme first requires the expression and purification of its constituent subunits, before reconstitution of the multisubunit complex. Previously, purification of the HsdM protein had proved problematic, due to the length of time required for the purification and its susceptibility to degradation. A new protocol was therefore developed to decrease the length of time required to purify the HsdM protein and thus prevent degradation. Finally, we show that the HsdM subunit exhibits a concentration dependent monomer-dimer equilibrium.
authors.affiliation: Institute of Biomedical & Biomolecular Science, School of Biological Sciences, University of Portsmouth, King Henry I St., Portsmouth PO1 2DY, United Kingdom.
authors.forename: James E N
authors.initials: JEN
authors.lastname: Taylor
authors.affiliation: Institute of Biomedical & Biomolecular Science, School of Biological Sciences, University of Portsmouth, King Henry I St., Portsmouth PO1 2DY, United Kingdom.
authors.forename: Anna
authors.initials: A
authors.lastname: Swiderska
authors.affiliation: Institute of Biomedical & Biomolecular Science, School of Biological Sciences, University of Portsmouth, King Henry I St., Portsmouth PO1 2DY, United Kingdom.
authors.forename: G Geoff
authors.initials: GG
authors.lastname: Kneale
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D015280:DNA Restriction-Modification Enzymes / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D015253:Deoxyribonucleases, Type I Site-Specific / Q000737:chemistry*; D004926:Escherichia coli; D008780:Methyltransferases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D011479:Protamines / Q000737:chemistry; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012965:Sodium Chloride / Q000737:chemistry; D014461:Ultracentrifugation
chemical_list: D001426:Bacterial Proteins; D015280:DNA Restriction-Modification Enzymes; D011479:Protamines; D021122:Protein Subunits; D011994:Recombinant Proteins; D012965:Sodium Chloride; D008780:Methyltransferases; C109829:HsdM protein, Bacteria; C048242:endodeoxyribonuclease EcoR124I; D015253:Deoxyribonucleases, Type I Site-Specific