title: Effect of the amino acid substitution in the DNA-binding domain of the Fur regulator on production of pyoverdine.
abstract: The ferric uptake regulator gene (fur), its promoter region and Fur box of pvdS gene involved in siderophore-mediated iron uptake system were sequenced in the parent strain Pseudomonas aeruginosa PAO1 and in the fur mutant FPA121 derived from the strain PAO1. We identified the gene fur 179 bearing a novel, single-point mutation that changed the amino acid residue Gln60Pro in the DNA-binding domain of the Fur protein. The synthesis of pyoverdine was studied in cultures of the strains PAO1 and FPA121 grown in iron-deplete and iron-replete (60 μmol/L FeIII) medium. The amino acid replacement in the regulatory Fur protein is responsible for the overproduction of pyoverdine in iron-deplete and iron-replete medium. No mutation was identified in the Fur box of the gene pvdS.
authors.affiliation: Laboratory of Enzyme Technology, Institute of Microbiology v.v.i, Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague, Czech Republic.
authors.forename: Renáta
authors.initials: R
authors.lastname: Valešová
authors.forename: Andrea
authors.initials: A
authors.lastname: Palyzová
authors.forename: Helena
authors.initials: H
authors.lastname: Marešová
authors.forename: Václav
authors.initials: V
authors.lastname: Stěpánek
authors.forename: Peter
authors.initials: P
authors.lastname: Babiak
authors.forename: Pavel
authors.initials: P
authors.lastname: Kyslík
mesh_terms: D019943:Amino Acid Substitution; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001665:Binding Sites; D003470:Culture Media / Q000737:chemistry; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005290:Ferric Compounds / Q000378:metabolism; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D009842:Oligopeptides / Q000096:biosynthesis*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D004268:DNA-Binding Proteins; D005290:Ferric Compounds; D050505:Mutant Proteins; D009842:Oligopeptides; D012097:Repressor Proteins; C068477:ferric uptake regulating proteins, bacterial; C042453:pyoverdin