title: Rosamine-based fluorescent sensor with femtomolar affinity for the reversible detection of a mercury ion.
abstract: A Hg(2+)-selective fluorescent sensor, RosHg, has been developed based on a rosamine platform. RosHg exhibited a ∼20-fold increase in fluorescence emission upon binding with Hg(2+), and the enhanced fluorescence was immediately decreased when glutathione was added to a solution of the Hg-RosHg complex. The dissociation constant for the Hg(2+) complex was determined to be 0.10 fM by using a set of Hg(2+)/Mg(2+)/ethylenediaminetetraacetic acid buffer solutions. Confocal microscopy experiments demonstrated that this sensor can monitor changes of the Hg(2+) level in the mitochondria of living cells.
authors.affiliation: Graduate School of Human & Environmental Studies, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan. taki.masayasu.4c@kyoto-u.ac.jp
authors.forename: Masayasu
authors.initials: M
authors.lastname: Taki
authors.forename: Kazushi
authors.initials: K
authors.lastname: Akaoka
authors.forename: Shohei
authors.initials: S
authors.lastname: Iyoshi
authors.forename: Yukio
authors.initials: Y
authors.lastname: Yamamoto
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007477:Ions; D008628:Mercury / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000737:chemistry; D015394:Molecular Structure; D009930:Organic Chemicals / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D007477:Ions; D009930:Organic Chemicals; C107472:red dye CMXRos; D008628:Mercury