title: Assembly of discrete collagen-chitosan microenvironments into multiphase tissue constructs.
abstract: Modular assembly of protein-polysaccharide microenvironments into 3D macroscale tissue constructs is reported. Rapid and simple centrifugation and vacuum molding methods are used to create cohesive multiphase constructs with prescribed geometries. Human fibroblasts are shown to survive in the microenvironments and in the macroscale constructs. Control of the spatial organization in engineered tissues is a key to recreating the complex tissue architectures needed for regenerative therapies.
authors.affiliation: Department of Biomedical Engineering, University of Michigan, 1101 Beal Ave. Ann Arbor, MI 48109, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Caldwell
authors.forename: Rameshwar R
authors.initials: RR
authors.lastname: Rao
authors.forename: Jan P
authors.initials: JP
authors.lastname: Stegemann
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis*; D040761:Biomimetic Materials / Q000737:chemistry*; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D060833:Cellular Microenvironment; D048271:Chitosan / Q000737:chemistry*; D024042:Collagen Type I / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D008422:Materials Testing; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D024042:Collagen Type I; D048271:Chitosan