title: [Comparison and analysis of two methods for serum free prostate-specific antigen (PSA) detection].
abstract: OBJECTIVE
  To analyze the clinical performance of free prostate-specific antigen (fPSA) detection by ECLIA method, and evaluate whether ECLIA is suitable for clinical use.
  
  
  METHODS
  341 samples were collected and tested prostate-specific antibodies with CMIA and ECLIA methods. These samples contain: 97 samples with abnormal high PSA value tested by CMIA method, and 244 normal PSA samples. Use CMIA as the reference method, and detect fPSA, tPSA levels, and the ratio of fPSA/tPSA. Analyze the testing results with statistical methods.
  
  
  RESULTS
  Compared with CMIA, correlation coefficent of ECLIA fPSA detection is 0.99; correlation coefficent of f/tPSA ratio detection is 0.96; the sensitivity, specificity of ECLIA f/tPSA ratio detection are 85.71%, 92.6% respectively, the agreement rate with ECLIA is 87.4%. No cross reaction with bilirubin, lipohemia, hemolysis, RF, CEA, AFP, CA125, CA153, CA199 were found in the tests.
  
  
  CONCLUSIONS
  The ECLIA method for free prostate-specific antigen detection showed good clinical performance; and is suitable for clinical use.
authors.affiliation: Department of Laboratory Medicine, Beijing Hospital, Beijing 100730, China.
authors.forename: Yu-Qing
authors.initials: YQ
authors.lastname: Jiang
authors.forename: Zhi-Xian
authors.initials: ZX
authors.lastname: Xie
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Tian-Yi
authors.initials: TY
authors.lastname: Liu
authors.forename: Zheng-Ran
authors.initials: ZR
authors.lastname: Ma
authors.forename: Hao-Jia
authors.initials: HJ
authors.lastname: Zhi
mesh_terms: D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D008297:Male; D017430:Prostate-Specific Antigen / Q000097:blood*; D011471:Prostatic Neoplasms / Q000097:blood / Q000175:diagnosis*; D012680:Sensitivity and Specificity
chemical_list: D017430:Prostate-Specific Antigen