title: DNA damage, p53, Ki-67 and COX-2 expression in rat tongue cells exposed to nandrolone decanoate.
abstract: The objective of this article was to evaluate the impact potential of nandrolone decanoate on DNA damage, cellular regulatory proteins and cyclooxygenase (COX)-2 in oral mucosa cells of Wistar rats. A total of 40 rats were distributed into four groups. Two experimental groups were treated with nandrolone decanoate, at 5 mg/kg doses, subcutaneously, three times a week in two periods: 15 and 30 days. The remaining groups received only 0.9% saline subcutaneously, three times a week. To evaluate genetic damage, nandrolone decanoate at 15 mg/kg dose was exposed to 24 h. In the histopathological analysis, no remarkable morphological changes were observed in tongue tissue in all groups. Significant increase in immunoexpression of Ki-67, p53, COX-2 proteins was detected in the groups treated with nandrolone decanoate during 15 and 30 days, when compared to their respective controls. A positive correlation between immunoexpression of p53 and COX-2 protein was detected following nandrolone decanoate exposure. DNA damage was induced by nandrolone decanoate in oral mucosa cells at 15 mg/kg dose. Our results suggest that nandrolone decanoate was able to alter the expression of cell cycle-related proteins, as well as to induce genetic damage and COX-2 immunoexpression in tongue cells of Wistar rats.
authors.affiliation: Department of Biosciences, Federal University of São Paulo, São Paulo SP 11060-001, Brazil.
authors.forename: Renan
authors.initials: R
authors.lastname: Pozzi
authors.forename: Kelly Rosseti
authors.initials: KR
authors.lastname: Fernandes
authors.forename: Carolina
authors.initials: C
authors.lastname: Foot Gomes de Moura
authors.forename: Raquel Agnelli Mesquita
authors.initials: RA
authors.lastname: Ferrari
authors.forename: Kristianne Porta Santos
authors.initials: KP
authors.lastname: Fernandes
authors.forename: Marcelo Donizeti
authors.initials: MD
authors.lastname: Chaves
authors.forename: Ana Claudia Muniz
authors.initials: AC
authors.lastname: Renno
authors.forename: Daniel Araki
authors.initials: DA
authors.lastname: Ribeiro
mesh_terms: D045930:Anabolic Agents / Q000633:toxicity*; D000818:Animals; D020552:Comet Assay; D051546:Cyclooxygenase 2 / Q000096:biosynthesis*; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000096:biosynthesis*; D008297:Male; D009061:Mouth Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D009277:Nandrolone / Q000031:analogs & derivatives* / Q000633:toxicity; D000077603:Nandrolone Decanoate; D051381:Rats; D017208:Rats, Wistar; D014059:Tongue / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*
chemical_list: D045930:Anabolic Agents; D019394:Ki-67 Antigen; D016159:Tumor Suppressor Protein p53; D009277:Nandrolone; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat; D000077603:Nandrolone Decanoate