title: A set of plastid microsatellite loci for the genus Dyckia (Bromeliaceae) derived from 454 pyrosequencing.
abstract: OBJECTIVE
  Phylogeographical analyses of Dyckia (Bromeliaceae) suffer from low levels of sequence variation. Plastid microsatellite markers were developed to achieve a better-resolved genus-wide plastid genealogy of Dyckia. •
  
  
  RESULTS
  Approximately 84% of the D. marnier-lapostollei plastome was sequenced using 454 technology. Flanking primer pairs were designed for 34 out of 36 chloroplast simple sequence repeats (cpSSRs) detected, and 12 loci were further characterized by genotyping Dyckia samples at the level of populations and species. Three, five, and six cpSSRs were polymorphic among four individuals of D. limae, 12 individuals of D. dissitiflora, and 12 of D. pernambucana, respectively, with two to three alleles per locus and species. All loci were polymorphic among 19 different Dyckia species, with three to 10 alleles per locus. Ten primer pairs cross-amplified with bromeliad genera from five subfamilies. •
  
  
  CONCLUSIONS
  The set of 12 cpSSR markers provides a toolbox to analyze phylogeographical patterns of Dyckia species.
authors.affiliation: Plant Molecular Systematics, Department of Sciences, University of Kassel, D-34132 Kassel, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Krapp
authors.forename: Tina
authors.initials: T
authors.lastname: Wöhrmann
authors.forename: Diego Sotero de Barros
authors.initials: DS
authors.lastname: Pinangé
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Benko-Iseppon
authors.forename: Bruno
authors.initials: B
authors.lastname: Huettel
authors.forename: Kurt
authors.initials: K
authors.lastname: Weising
mesh_terms: D001938:Brazil; D031229:Bromeliaceae / Q000235:genetics*; D002736:Chloroplasts / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D010802:Phylogeny; D058974:Phylogeography; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018744:DNA, Plant