title: Proteome-based profiling of hypercellulase-producing strains developed through interspecific protoplast fusion between Aspergillus nidulans and Aspergillus tubingensis.
abstract: Thirty heterokaryons, formed by protoplast fusion of Aspergillus nidulans and Aspergillus tubingensis, were selected on the basis of their ability to grow on 2-deoxyglucose (0.2 %, w/v) and intermediate spore color. These heterokaryons were studied for cellulase production using shake flask and solid substrate cultures at 40 °C. Fusants 51 and 28 exhibited appreciably higher levels of endoglucanase, cellobiohydrolase, β-glucosidase, and FPase activities when compared with parental strains. Employing proteomic-based approaches, the differential expression of proteins in secretome of fusants and parental strains were analyzed using two-dimensional electrophoresis. The expression of some of the proteins in the fusants was found to be up/downregulated. The upregulated proteins in the fusant 51 were identified by liquid chromatography-mass spectroscopy as endoxylanase, endochitinase, β-glucosidase, as well as hypothetical proteins. The cellulases produced by fusants 28 and 51 showed improved saccharification of alkali treated rice straw when compared with the parental strains.
authors.affiliation: Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab 143 005, India. baljit_sohal@rediffmail.com
authors.forename: Baljit
authors.initials: B
authors.lastname: Kaur
authors.forename: Manju
authors.initials: M
authors.lastname: Sharma
authors.forename: Rohit
authors.initials: R
authors.lastname: Soni
authors.forename: H S
authors.initials: HS
authors.lastname: Oberoi
authors.forename: B S
authors.initials: BS
authors.lastname: Chadha
mesh_terms: D001230:Aspergillus / Q000145:classification* / Q000254:growth & development*; D002459:Cell Fusion / Q000379:methods*; D002480:Cellulase / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D020543:Proteome / Q000235:genetics*; D011523:Protoplasts / Q000502:physiology*; D013045:Species Specificity
chemical_list: D005656:Fungal Proteins; D020543:Proteome; D002480:Cellulase