title: Immunohistochemical expression of p63, epidermal growth factor receptor (EGFR) and notch-1 in radicular cysts, dentigerous cysts and keratocystic odontogenic tumors.
abstract: The aim of this study was to assess the immunohistochemical expression of p63 protein, epidermal growth factor receptor (EGFR) and Notch-1 in the epithelial lining of radicular cysts (RC), dentigerous cysts (DC) and keratocystic odontogenic tumors (KOT). For this study, 35 RC, 22 DC and 17 KOT were used. The clinical and epidemiological data were collected from the patient charts filed in the Oral Pathology Laboratory, University of Ribeirão Preto, Brazil. Immunohistochemical reactions against the p63, EGFR and Notch-1 were performed in 3-µm-thick histological sections. The slides were evaluated according to the following criteria: negative: <5% of positive cells, low expression: 5-50% of positive cells, and high expression: >50% of positive cells. Moreover, the intensity of EGFR and Notch-1 expressions was also evaluated. Fisher's exact test and Spearman's correlation coefficients were used for statistical analysis, considering a significance level of 5%. Almost all cases demonstrated p63, EGFR and Notch-1 expressions. The p63 expression was significantly higher in KOT (p<0.001). Positive correlation between these immunomarkers was observed. These findings suggest the participation of p63, EGFR and Notch-1 in the development, maintenance and integrity of cystic odontogenic epithelial lining, favoring lesion persistence. The high expression of p63 in KOT suggests that it may be related to their more aggressive biological behavior and marked tendency to recurrence.
authors.affiliation: Department of Clinical and Preventive Dentistry, Oral Pathology Section, Dental School, Federal University of Pernambuco, Recife, PE, Brazil.
authors.forename: Cláudia Kallás
authors.initials: CK
authors.lastname: Gonçalves
authors.forename: Eduardo Rodrigues
authors.initials: ER
authors.lastname: Fregnani
authors.forename: Jorge Esquiche
authors.initials: JE
authors.lastname: Leon
authors.forename: Yara Teresinha Corrêa
authors.initials: YT
authors.lastname: Silva-Sousa
authors.forename: Danyel Elias da Cruz
authors.initials: DE
authors.lastname: Perez
mesh_terms: D015415:Biomarkers / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis; D002462:Cell Membrane / Q000473:pathology; D002467:Cell Nucleus / Q000473:pathology; D003593:Cytoplasm / Q000473:pathology; D003803:Dentigerous Cyst / Q000473:pathology*; D004847:Epithelial Cells / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D066246:ErbB Receptors / Q000032:analysis*; D005456:Fluorescent Dyes; D006801:Humans; D006965:Hyperplasia; D007150:Immunohistochemistry; D009364:Neoplasm Recurrence, Local / Q000473:pathology; D009808:Odontogenic Tumors / Q000473:pathology*; D011842:Radicular Cyst / Q000473:pathology*; D051881:Receptor, Notch1 / Q000032:analysis*; D012189:Retrospective Studies; D014157:Transcription Factors / Q000032:analysis*; D025521:Tumor Suppressor Proteins / Q000032:analysis*
chemical_list: D015415:Biomarkers; D014408:Biomarkers, Tumor; D005456:Fluorescent Dyes; C497761:NOTCH1 protein, human; D051881:Receptor, Notch1; C115051:TP63 protein, human; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D066246:ErbB Receptors