title: Revising lysenin expression of earthworm coelomocytes.
abstract: Lysenin is a species-specific bioactive molecule of Eisenia andrei earthworms. This protein is a potent antimicrobial factor; however its cellular expression and induction against pathogens are still not fully understood. We developed a novel monoclonal antibody against lysenin and applied this molecular tool to characterize its production and antimicrobial function. We demonstrated by flow cytometry and immunocytochemistry that one subgroup of earthworm immune cells (so called coelomocytes), the chloragocytes expressed the highest amount of lysenin. Then, we compared lysenin expression with earlier established coelomocyte (EFCC) markers. In addition, we determined by immunohistology of earthworm tissues that lysenin production is only restricted to free-floating chloragocytes. Moreover, we observed that upon in vitro Staphylococcus aureus but not Escherichia coli challenged coelomocytes over-expressed and then secreted lysenin. These results indicate that among subpopulations of coelomocytes, lysenin is mainly produced by chloragocytes and its expression can be modulated by Gram-positive bacterial exposure.
authors.affiliation: Department of Immunology and Biotechnology, Clinical Center, University of Pécs, Szigeti u. 12, Pécs, Hungary.
authors.forename: Balázs
authors.initials: B
authors.lastname: Opper
authors.forename: András
authors.initials: A
authors.lastname: Bognár
authors.forename: Diána
authors.initials: D
authors.lastname: Heidt
authors.forename: Péter
authors.initials: P
authors.lastname: Németh
authors.forename: Péter
authors.initials: P
authors.lastname: Engelmann
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism*; D002469:Cell Separation; D002478:Cells, Cultured; D004926:Escherichia coli / Q000276:immunology*; D004927:Escherichia coli Infections / Q000276:immunology*; D005434:Flow Cytometry; D007110:Immunity, Active; D007150:Immunohistochemistry; D009835:Oligochaeta / Q000276:immunology* / Q000382:microbiology; D013203:Staphylococcal Infections / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology*; D014118:Toxins, Biological / Q000276:immunology / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D014118:Toxins, Biological; C107040:lysenin