title: Complete sequence of the first chimera genome constructed by cloning the whole genome of Synechocystis strain PCC6803 into the Bacillus subtilis 168 genome.
abstract: Genome synthesis of existing or designed genomes is made feasible by the first successful cloning of a cyanobacterium, Synechocystis PCC6803, in Gram-positive, endospore-forming Bacillus subtilis. Whole-genome sequence analysis of the isolate and parental B. subtilis strains provides clues for identifying single nucleotide polymorphisms (SNPs) in the 2 complete bacterial genomes in one cell.
authors.affiliation: Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Watanabe
authors.forename: Yuh
authors.initials: Y
authors.lastname: Shiwa
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Itaya
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yoshikawa
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D046939:Synechocystis / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial