title: Role of Rev-erbα domains for transactivation of the connexin43 promoter with Sp1.
abstract: Rev-erbα, a component of the circadian clock, has also been known as a nuclear receptor that lacks activation function domain 2, functioning as a ligand-dependent transcriptional repressor. However, we recently reported that Rev-erbα activates connexin43 transcription by forming a complex with Sp1. Here we show that heme, a REV-ERB ligand, is dispensable for this novel mechanism and that Rev-erbβ, having homologies with Rev-erbα, does not activate connexin43, but competes with the Rev-erbα/Sp1. The A/B region of Rev-erbα, which is not conserved in Rev-erbβ, is a crucial activating domain, while the ligand binding domain, conserved in Rev-erbβ, functions as a competitor.
authors.affiliation: Department of Urology, Kyoto University, Graduate School of Medicine, Sakyo, Kyoto 606-8507, Japan.
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Negoro
authors.forename: Takeshi
authors.initials: T
authors.lastname: Okinami
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kanematsu
authors.forename: Masaaki
authors.initials: M
authors.lastname: Imamura
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
authors.forename: Osamu
authors.initials: O
authors.lastname: Ogawa
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D018031:Connexin 43 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D017930:Genes, Reporter; D057809:HEK293 Cells; D006418:Heme / Q000378:metabolism; D006801:Humans; D008024:Ligands; D051379:Mice; D016254:Mutagenesis, Insertional; D016297:Mutagenesis, Site-Directed; D050505:Mutant Proteins / Q000737:chemistry / Q000378:metabolism; D056953:Nuclear Receptor Subfamily 1, Group D, Member 1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D054730:Protein Interaction Domains and Motifs; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016329:Sp1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D018031:Connexin 43; C543076:GJA1 protein, mouse; D008024:Ligands; D050505:Mutant Proteins; C498262:Nr1d1 protein, mouse; C499650:Nr1d2 protein, mouse; D056953:Nuclear Receptor Subfamily 1, Group D, Member 1; D018160:Receptors, Cytoplasmic and Nuclear; D011994:Recombinant Proteins; D012097:Repressor Proteins; D016329:Sp1 Transcription Factor; D006418:Heme