title: Ectoenzymes and cholinesterase activity and biomarkers of oxidative stress in patients with lung cancer.
abstract: We aimed to examine the nucleoside triphosphate diphosphohydrolases (NTPDase) in lymphocytes; adenosine deaminase (ADA) and butyrylcholinesterase (BChE) in serum; and acetylcholinesterase (AChE), superoxide dismutase (SOD), and catalase (CAT) activity in whole blood; since these enzymes are involved in inflammation responses as well as in oxidative stress conditions. We also checked the levels of total thiols (T-SH), non-protein thiols (NPSH), and thiobarbituric acid reactive substances (TBARS) in serum of patients with lung cancer. We collected blood samples from patients (n = 31) previously treated for lung cancer with chemotherapy. Patients were classified as stage IIIb and IV according to the Union for International Cancer Control (UICC). The results showed a significant increase in the hydrolysis of ATP, ADP, and adenosine in patients when compared with the control group. The activity of AChE, SOD, and CAT as well as the T-SH and NPSH levels were higher in patients group and TBARS levels were lower in patients compared with the control group. These findings demonstrated that the enzymes activity involved in the control of inflammatory and immune processes as well as the oxidative stress parameters are altered in patients with lung cancer.
authors.affiliation: Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Departamento de Química, Universidade Federal de Santa Maria, Campus Universitário, Camobi, Santa Maria, RS, 97105-900, Brazil. dz_daniela@yahoo.com.br
authors.forename: Daniela
authors.initials: D
authors.lastname: Zanini
authors.forename: Roberta
authors.initials: R
authors.lastname: Schmatz
authors.forename: Luana Paula
authors.initials: LP
authors.lastname: Pelinson
authors.forename: Victor Camera
authors.initials: VC
authors.lastname: Pimentel
authors.forename: Pauline
authors.initials: P
authors.lastname: da Costa
authors.forename: Andréia Machado
authors.initials: AM
authors.lastname: Cardoso
authors.forename: Caroline Curry
authors.initials: CC
authors.lastname: Martins
authors.forename: Christina Chitolina
authors.initials: CC
authors.lastname: Schetinger
authors.forename: Jucimara
authors.initials: J
authors.lastname: Baldissareli
authors.forename: Maria
authors.initials: M
authors.lastname: do Carmo Araújo
authors.forename: Liliane
authors.initials: L
authors.lastname: Oliveira
authors.forename: Juarez
authors.initials: J
authors.lastname: Chiesa
authors.forename: Vera Maria
authors.initials: VM
authors.lastname: Morsch
authors.forename: Daniela Bitencourt Rosa
authors.initials: DB
authors.lastname: Leal
authors.forename: Maria Rosa Chitolina
authors.initials: MR
authors.lastname: Schetinger
mesh_terms: D000110:Acetylcholinesterase / Q000097:blood; D000243:Adenosine Deaminase / Q000097:blood; D000368:Aged; D000970:Antineoplastic Agents / Q000627:therapeutic use; D014408:Biomarkers, Tumor / Q000097:blood*; D002091:Butyrylcholinesterase / Q000097:blood; D002374:Catalase / Q000097:blood; D002802:Cholinesterases / Q000097:blood* / Q000378:metabolism; D002945:Cisplatin / Q000627:therapeutic use; D003841:Deoxycytidine / Q000031:analogs & derivatives / Q000627:therapeutic use; D005260:Female; D006801:Humans; D007249:Inflammation / Q000201:enzymology*; D008175:Lung Neoplasms / Q000188:drug therapy / Q000201:enzymology / Q000378:metabolism*; D008214:Lymphocytes / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D043583:Nucleoside-Triphosphatase / Q000378:metabolism; D018384:Oxidative Stress; D012907:Smoking / Q000097:blood; D013438:Sulfhydryl Compounds / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D000093542:Gemcitabine
chemical_list: D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D003841:Deoxycytidine; D002374:Catalase; D013482:Superoxide Dismutase; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D002802:Cholinesterases; D000243:Adenosine Deaminase; D043583:Nucleoside-Triphosphatase; D002945:Cisplatin; D000093542:Gemcitabine