title: Mouse dendritic cell (DC) influenza virus infectivity is much lower than that for human DCs and is hemagglutinin subtype dependent.
abstract: We show that influenza A H1N1 virus infection leads to very low infectivity in mouse dendritic cells (DCs) in vitro compared with that in human DCs. This holds when H3 or H5 replaces H1 in recombinant viruses. Viruslike particles confirm the difference between mouse and human, suggesting that reduced virus entry contributes to lower mouse DC infectivity. Low infectivity of mouse DCs should be considered when they are used to study responses of DCs that are actually infected.
authors.affiliation: Department of Neurology, Mount Sinai School of Medicine, New York, New York, USA.
authors.forename: Boris M
authors.initials: BM
authors.lastname: Hartmann
authors.forename: Wenjing
authors.initials: W
authors.lastname: Li
authors.forename: Jingjing
authors.initials: J
authors.lastname: Jia
authors.forename: Sonali
authors.initials: S
authors.lastname: Patil
authors.forename: Nada
authors.initials: N
authors.lastname: Marjanovic
authors.forename: Carles
authors.initials: C
authors.lastname: Martínez-Romero
authors.forename: Randy A
authors.initials: RA
authors.lastname: Albrecht
authors.forename: Fernand
authors.initials: F
authors.lastname: Hayot
authors.forename: Adolfo
authors.initials: A
authors.lastname: García-Sastre
authors.forename: James G
authors.initials: JG
authors.lastname: Wetmur
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Moran
authors.forename: Stuart C
authors.initials: SC
authors.lastname: Sealfon
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000821:virology*; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000472:pathogenicity*; D051379:Mice; D053586:Virus Internalization
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; C109963:hemagglutinin, avian influenza A virus