title: Bioaugmented remediation of high concentration BTEX-contaminated groundwater by permeable reactive barrier with immobilized bead.
abstract: Ineffective biostimulation requires immediate development of new technologies for remediation of high concentration BTEX-contaminated (benzene, toluene, ethylbenzene and xylene) groundwater. In this study, bioaugmentation with Mycobacterium sp. CHXY119 and Pseudomonas sp. YATO411 immobilized bead was used to remediate BTEX-contaminated groundwater with about 100 mg l(-1) in total concentration. The batch test results showed that the CHXY119 and YATO411 immobilized bead completely biodegraded each BTEX compound, and the maximum biodegradation rates were 0.790 mg l(-1) h(-1) for benzene, 1.113 mg l(-1) h(-1) for toluene, 0.992 mg l(-1) h(-1) for ethylbenzene and 0.231 mg l(-1) h(-1) for p-xylene. The actual mineralization rates were 10.8% for benzene, 10.5% for toluene, 5.8% for ethylbenzene and 11.4% for p-xylene, which indicated that the bioremediation of BTEX by the immobilized bead requires a rather small oxygen supply. Degradation rates achieved by the bioaugmented permeable reactive barrier (Bio-PRB) system of the immobilized bead were 97.8% for benzene, 94.2% for toluene, 84.7% for ethylbenzene and 87.4% for p-xylene; and the toxicity of the groundwater fell by 91.2% after bioremediation by the bioaugmented PRB, which confirmed its great potential for remediating groundwater with high concentrations of contaminants.
authors.affiliation: Department of Environmental Engineering and Energy, School of Chemical Engineering and Environment, Beijing Institute of Technology, Beijing 100081, PR China.
authors.forename: Bao-Ping
authors.initials: BP
authors.lastname: Xin
authors.forename: Chih-Hung
authors.initials: CH
authors.lastname: Wu
authors.forename: Cheng-Han
authors.initials: CH
authors.lastname: Wu
authors.forename: Chi-Wen
authors.initials: CW
authors.lastname: Lin
mesh_terms: D000464:Alginates / Q000737:chemistry; D001555:Benzene Derivatives / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000235:genetics; D020723:Glucuronic Acid / Q000737:chemistry; D060587:Groundwater; D006603:Hexuronic Acids / Q000737:chemistry; D009161:Mycobacterium / Q000235:genetics / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D011142:Polyvinyl Alcohol / Q000737:chemistry; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D000464:Alginates; D001555:Benzene Derivatives; D004269:DNA, Bacterial; D006603:Hexuronic Acids; D014874:Water Pollutants, Chemical; D020723:Glucuronic Acid; D011142:Polyvinyl Alcohol; D010100:Oxygen