title: RNA-based assessment of diversity and composition of active archaeal communities in the German Bight.
abstract: Archaea play an important role in various biogeochemical cycles. They are known extremophiles inhabiting environments such as thermal springs or hydrothermal vents. Recent studies have revealed a significant abundance of Archaea in moderate environments, for example, temperate sea water. Nevertheless, the composition and ecosystem function of these marine archaeal communities is largely unknown. To assess diversity and composition of active archaeal communities in the German Bight, seven marine water samples were taken and studied by RNA-based analysis of ribosomal 16S rRNA. For this purpose, total RNA was extracted from the samples and converted to cDNA. Archaeal community structures were investigated by pyrosequencing-based analysis of 16S rRNA amplicons generated from cDNA. To our knowledge, this is the first study combining next-generation sequencing and metatranscriptomics to study archaeal communities in marine habitats. The pyrosequencing-derived dataset comprised 62,045 archaeal 16S rRNA sequences. We identified Halobacteria as the predominant archaeal group across all samples with increased abundance in algal blooms. Thermoplasmatales (Euryarchaeota) and the Marine Group I (Thaumarchaeota) were identified in minor abundances. It is indicated that archaeal community patterns were influenced by environmental conditions.
authors.affiliation: Department of Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August University of Göttingen, Grisebachstraße 8, 37077 Göttingen, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Wemheuer
authors.forename: Franziska
authors.initials: F
authors.lastname: Wemheuer
authors.forename: Rolf
authors.initials: R
authors.lastname: Daniel
mesh_terms: D001105:Archaea / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D044822:Biodiversity; D005858:Germany; D054892:Metagenome; D008969:Molecular Sequence Data; D019642:RNA, Archaeal / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D019642:RNA, Archaeal; D012336:RNA, Ribosomal, 16S