title: Anisotropic collagen fibrillogenesis within microfabricated scaffolds: implications for biomimetic tissue engineering.
abstract: Anisotropic collagen fibrillogenesis is demonstrated within the pores of an accordion-like honeycomb poly(glycerol sebacate) tissue engineering scaffold. Confocal reflectance microscopy and image analysis demonstrate increased fibril distribution order, fibril density, and alignment in accordion-like honeycomb pores compared with collagen gelled unconstrained. Finite element modeling predicts how collagen gel and scaffold mechanics couple in matching native heart muscle stiffness and anisotropy.
authors.affiliation: Department of Bioengineering, The Pennsylvania State University, 223 Hallowell Building, University Park, PA 16802, USA.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Jean
authors.forename: George C
authors.initials: GC
authors.lastname: Engelmayr
mesh_terms: D016880:Anisotropy; D040761:Biomimetic Materials / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry* / Q000648:ultrastructure*; D005109:Extracellular Matrix / Q000737:chemistry* / Q000648:ultrastructure*; D008904:Miniaturization; D046912:Multiprotein Complexes / Q000737:chemistry / Q000648:ultrastructure; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D046912:Multiprotein Complexes; D003094:Collagen