title: Efficacy of mesoporous silica nanoparticles in delivering BMP-2 plasmid DNA for in vitro osteogenic stimulation of mesenchymal stem cells.
abstract: We report the ability of aminated mesoporous silica nanoparticles (MSN-NH2) with large mesopore space and positive-charged surface to deliver genes within rat mesenchymal stem cells (MSCs). The amine functionalized inorganic nanoparticles were complexed with bone morphogenetic protein-2 (BMP2) plasmid DNA (pDNA) to study their transfection efficiency in MSCs. Intracellular uptake of the complex BMP2 pDNA/MSN-NH2 occurred significantly, with a transfection efficiency of approximately 68%. Furthermore, over 66% of the transfected cells produced BMP2 protein. The osteogenic differentiation of the transfected MSCs was demonstrated by the expression of bone-related genes and proteins including bone sialoprotein, osteopontin, and osteocalcin. The MSN-NH2 delivery vehicle for BMP2 pDNA developed in this study may be a potential gene delivery system for bone tissue regeneration.
authors.affiliation: Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, South Korea.
authors.forename: Tae-Hyun
authors.initials: TH
authors.lastname: Kim
authors.forename: Meeju
authors.initials: M
authors.lastname: Kim
authors.forename: Mohamed
authors.initials: M
authors.lastname: Eltohamy
authors.forename: Ye-Rang
authors.initials: YR
authors.lastname: Yun
authors.forename: Jun-Hyeog
authors.initials: JH
authors.lastname: Jang
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Kim
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism; D002452:Cell Count; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D058574:Integrin-Binding Sialoprotein / Q000235:genetics / Q000378:metabolism; D042541:Intracellular Space / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism*; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012822:Silicon Dioxide / Q000494:pharmacology*; D055672:Static Electricity; D014162:Transfection
chemical_list: D055396:Bone Morphogenetic Protein 2; D058574:Integrin-Binding Sialoprotein; D015675:Osteocalcin; D053495:Osteopontin; D012822:Silicon Dioxide; D004247:DNA