title: [Determination of carboxyatractyloside and atractyloside in Xanthii Fructus by HPLC].
abstract: OBJECTIVE
  To determine carboxyatractyloside and atractyloside in Xanthii Fructus by HPLC.
  
  
  METHODS
  By HPLC, Agilent ZORBAX SB-phenyl (4.6 mm x 250 mm, 5 microm) column was adopted, with acetonitrile-0.01 mol x L(-1) NaH2PO4 (pH 6) as the mobile phase for gradient elution at the flow rate of 1.0 mL x min(-1). The detection wavelength was 203 nm, and the temperature was set at 35 degrees C.
  
  
  RESULTS
  Carboxyatractyloside showed a good linearity within the range of 0.0972-1.944 microg and atractyloside showed a good linearity within the range of 0.1030-2.060 microg. The recovery rate of carboxyatractyloside was 100. 3% and that of atractyloside was 102.5%. The RSD were 0.67% and 1.4% (n=6).
  
  
  CONCLUSIONS
  This method is so simple, practical and highly repeatable that is can be used for quality control of Xanthii Fructus.
authors.affiliation: Sichuan Academy of Chinese Medicine Sciences, Chengdu 610041, China. 165185553@qq.com
authors.forename: Rui
authors.initials: R
authors.lastname: Duo
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Huang
authors.forename: Yunhua
authors.initials: Y
authors.lastname: Liu
authors.forename: Jinhai
authors.initials: J
authors.lastname: Yi
mesh_terms: D019659:Asteraceae / Q000737:chemistry*; D001278:Atractyloside / Q000031:analogs & derivatives* / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000302:isolation & purification; D005638:Fruit / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D001278:Atractyloside; C003853:carboxyatractyloside