title: 7-Ketocholesteryl-9-carboxynonanoate enhances the expression of ATP-binding cassette transporter A1 via CD36.
abstract: BACKGROUND
  CD36 signal transductions have been reported by a variety of lipid moiety of oxidized low-density lipoprotein (oxLDL), however, CD36 signal induced by 7-ketocholesteryl-9-carboxynonanoate (oxLig-1), a lipid moiety of oxLDL has not been elucidated.
  
  
  RESULTS
  OxLig-1 leads to activation and recruitment of Src kinase Fyn, Lyn and caveolin-1 to CD36 in J774A.1 cells, but not in CD36 knockdown cells. The mitogen-activated protein (MAP) kinases c-Jun N-terminal kinase 2 (JNK2) and extracellular signal-regulated protein kinase 1 and 2 (ERK1/2) are specifically phosphorylated in J774A.1 cells after treatment with oxLig-1 and inhibited by pretreatment of Src inhibitor, AG1879. The expression of ABCA1 is up-regulated by treatment with oxLig-1in J774A.1 cells, and the increased expression of ABCA1 is dramatically down-regulated by pretreatment with pharmacological inhibitors of ERK (PD98059) and JNK (SP600125).
  
  
  CONCLUSIONS
  The specific CD36 signal induced by oxLig-1 initiated the activation of Fyn, Lyn, caveolin-1, JNK2 and ERK1/2, and resulted in the up-regulation of ABCA1.
authors.affiliation: College of Life Science and Technology, Key Laboratory of Carbohydrate and Lipid Metabolism Research, Dalian University, 10-Xuefu Avenue, Dalian Economical and Technological Development Zone, Liaoning 116622, China.
authors.forename: Wenzhe
authors.initials: W
authors.lastname: Li
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Chi
authors.forename: Renjun
authors.initials: R
authors.lastname: Wang
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.forename: Guang
authors.initials: G
authors.lastname: Ma
authors.forename: Zilong
authors.initials: Z
authors.lastname: Chen
authors.forename: Jingda
authors.initials: J
authors.lastname: Li
authors.forename: Zhe
authors.initials: Z
authors.lastname: Liu
authors.forename: Eiji
authors.initials: E
authors.lastname: Matsuura
authors.forename: Qingping
authors.initials: Q
authors.lastname: Liu
mesh_terms: D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis* / Q000187:drug effects*; D018955:CD36 Antigens / Q000502:physiology*; D002788:Cholesterol Esters / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology*
chemical_list: C510905:7-ketocholesteryl-9-carboxynonanoate; D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters; D018955:CD36 Antigens; D002788:Cholesterol Esters