title: Functionalized hydrogen-bonding self-assembled monolayers grafted onto SiO2 substrates.
abstract: A novel urea coupling agent possessing a vinyl-terminal group and trimethoxysilyl anchoring group was synthesized and grafted onto SiO(2)/Au substrates. This ureido coupling agent exhibits a good capacity to directly yield homogeneous SAMs with a surface smoothing. Polarization modulation infrared reflection-absorption spectroscopy (PM-IRRAS) was used to monitor these SAMs. Indeed, the different functional groups (alkyl chain, urea, and vinyl) of this coupling agent were clearly observed in the PM-IRRAS spectra. Chemical modifications of the terminal function for the covalent immobilization of biomolecules were monitored by PM-IRRAS for the first time. We have demonstrated the successful reactions of the conversion of the vinyl-terminated SAMs successively into SAM-COOH and SAM-NHS without any degradation of the monolayer. The reactivity of activated esters was successfully investigated in order to immobilize the protein A.
authors.affiliation: Université de Bordeaux 1, ISM, UMR 5255, F-33400 Talence, France.
authors.forename: Michaël A
authors.initials: MA
authors.lastname: Ramin
authors.forename: Gwénaëlle
authors.initials: G
authors.lastname: Le Bourdon
authors.forename: Karine
authors.initials: K
authors.lastname: Heuzé
authors.forename: Marie
authors.initials: M
authors.lastname: Degueil
authors.forename: Colette
authors.initials: C
authors.lastname: Belin
authors.forename: Thierry
authors.initials: T
authors.lastname: Buffeteau
authors.forename: Bernard
authors.initials: B
authors.lastname: Bennetau
authors.forename: Luc
authors.initials: L
authors.lastname: Vellutini
mesh_terms: D000588:Amines / Q000737:chemistry; D002264:Carboxylic Acids / Q000737:chemistry; D005030:Ethylenes / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006860:Hydrogen Bonding; D055767:Immobilized Proteins / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D013205:Staphylococcal Protein A / Q000737:chemistry; D013499:Surface Properties; D014508:Urea / Q000737:chemistry
chemical_list: D000588:Amines; D002264:Carboxylic Acids; D005030:Ethylenes; D055767:Immobilized Proteins; D013205:Staphylococcal Protein A; D006046:Gold; D012822:Silicon Dioxide; D014508:Urea; C036216:ethylene; C004744:decylamine