title: Genome, epigenome and transcriptome analyses of a pair of monozygotic twins discordant for systemic lupus erythematosus.
abstract: Information to distinguish genetic and environmental factors in the pathogenesis of multifactorial diseases can be obtained by investigation of disease development in monozygotic twins. Recent reports have shown that there are genomic and epigenomic differences between monozygotic twins. Genomic/epigenomic and gene expression analyses were performed in monozygotic twins discordant for systemic lupus erythematosus (SLE) to find the genes playing important roles in SLE pathogenesis. Single nucleotide polymorphism (SNP) and copy number variation (CNV) typing, CpG methylation and gene expression were analyzed. The discordances in SNPs and CNVs were not confirmed. Both CpG methylation and gene expression levels were different for 10 genes. There were no genomic differences between monozygotic twins discordant for SLE, but epigenomic and gene expression differences were detected. These findings provide information for better understanding of SLE pathogenesis.
authors.affiliation: Clinical Research Center for Allergy and Rheumatology, Sagamihara Hospital, National Hospital Organization, Sagamihara, Japan. h-furukawa@sagamihara-hosp.gr.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Furukawa
authors.forename: Shomi
authors.initials: S
authors.lastname: Oka
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Matsui
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hashimoto
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Arinuma
authors.forename: Akiko
authors.initials: A
authors.lastname: Komiya
authors.forename: Naoshi
authors.initials: N
authors.lastname: Fukui
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Tsuchiya
authors.forename: Shigeto
authors.initials: S
authors.lastname: Tohma
mesh_terms: D000328:Adult; D018899:CpG Islands; D056915:DNA Copy Number Variations; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015894:Genome, Human; D054629:Genome, Mitochondrial; D005838:Genotype; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D014430:Twins, Monozygotic