title: Microsatellite markers developed for Corallodiscus lanuginosus (Gesneriaceae) and their cross-species transferability.
abstract: OBJECTIVE
  Corallodiscus is widespread across China and in adjacent countries to the west and south, with C. lanuginosus covering the entire range of the genus. Microsatellite markers will be useful to address within-complex taxonomic and biogeographic structures of this species. •
  
  
  RESULTS
  Fourteen markers were developed using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol. Polymorphism was assessed in 12 individuals each from two populations from West Yunnan, China. Nine primers displayed polymorphisms. The number of alleles per locus ranged from one to six (mean: 2.7). The observed and expected heterozygosities ranged from 0 to 1 and from 0 to 0.772 (average: 0.487 and 0.448). Only one of the polymorphic loci deviated from Hardy-Weinberg equilibrium. Among the four congeneric species, cross-amplification success ranged from two to eight markers. •
  
  
  CONCLUSIONS
  The markers will be useful for population genetic and evolutionary history studies for C. lanuginosus and the allied species C. bhutanicus, C. cooperi, and C. kingianus.
authors.affiliation: Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, Yunnan, People's Republic of China.
authors.forename: Lian-Ming
authors.initials: LM
authors.lastname: Gao
authors.forename: Zhi-Rong
authors.initials: ZR
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.forename: Michael
authors.initials: M
authors.lastname: Möller
authors.forename: De-Zhu
authors.initials: DZ
authors.lastname: Li
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D002681:China; D017931:DNA Primers / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D019684:Magnoliopsida / Q000235:genetics*; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018744:DNA, Plant