title: Effect of Colla corii asini (E'jiao) on D-galactose induced aging mice.
abstract: Colla corii asini (E'jiao), donkey-hide gelatin prepared by stewing and concentrating from Equus asinus L. donkey hide, is a traditional Chinese medicine preparation widely used in clinical hematic antanemic therapy in China. The aim of the present study was to investigate potential anti-aging effect of Colla corii asini and explore related mechanisms in D-galactose (gal) induced aging model mice. The mice were artificially induced aging by subcutaneously injection with D-gal at the dose of 100 mg/kg·d for 8 weeks. Colla corii asini was simultaneously treated to them once daily by intragastric gavage. Appetite, mental condition, body weight, and organ index were observed. Activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), as well as levels of malondialdehyde (MDA) in serum, brain, and liver were determined by according assay kits. Western blotting analysis was used to detect p16 and p21 expression. Results indicated that Colla corii asini could improve appetite, mental condition, body weight, and organ condition of model mice, improve SOD, CAT, and GSH-Px activities, reduce MDA levels, and modulate age-related genes expression in D-gal induced mice. Therefore, Colla corii asini may have effect to suppress the aging process through enhancing antioxidant activity, scavenging free radicals, and modulating aging-related gene expression.
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China.
authors.forename: Dongliang
authors.initials: D
authors.lastname: Wang
authors.forename: Maoxuan
authors.initials: M
authors.lastname: Liu
authors.forename: Jichao
authors.initials: J
authors.lastname: Cao
authors.forename: Yanna
authors.initials: Y
authors.lastname: Cheng
authors.forename: Chen
authors.initials: C
authors.lastname: Zhuo
authors.forename: Hongyan
authors.initials: H
authors.lastname: Xu
authors.forename: Shousheng
authors.initials: S
authors.lastname: Tian
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Fengshan
authors.initials: F
authors.lastname: Wang
mesh_terms: D000375:Aging / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D019588:Aging, Premature / Q000139:chemically induced; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D001066:Appetite / Q000187:drug effects*; D001835:Body Weight / Q000187:drug effects*; D001921:Brain / Q000187:drug effects*; D002374:Catalase / Q000378:metabolism; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D018397:Equidae; D005690:Galactose; D015870:Gene Expression / Q000187:drug effects*; D019942:Genes, p16; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D023421:Models, Animal; D009945:Organotherapy; D012867:Skin; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D050759:Cyclin-Dependent Kinase Inhibitor p21; D004365:Drugs, Chinese Herbal; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005690:Galactose