title: Expression of Paracoccidioides brasiliensis AMY1 in a Histoplasma capsulatum amy1 mutant, relates an α-(1,4)-amylase to cell wall α-(1,3)-glucan synthesis.
abstract: In the cell walls of the pathogenic yeast phases of Paracoccidioides brasiliensis, Blastomyces dermatitidis and Histoplasma capsulatum, the outer α-(1,3)-glucan layer behaves as a virulence factor. In H. capsulatum, an α-(1,4)-amylase gene (AMY1) is essential for the synthesis of this polysaccharide, hence related to virulence. An orthologous gene to H. capsulatum AMY1 was identified in P. brasiliensis and also labeled AMY1. P. brasiliensis AMY1 transcriptional levels were increased during the yeast phase, which correlates with the presence of α-(1,3)-glucan as the major yeast cell wall polysaccharide. Complementation of a H. capsulatum amy1 mutant strain with P. brasiliensis AMY1, suggests that P. brasiliensis Amy1p may play a role in the synthesis of cell wall α-(1,3)-glucan. To study some biochemical properties of P. brasiliensis Amy1p, the enzyme was overexpressed, purified and studied its activity profile with starch and amylopeptin. It showed a relatively higher hydrolyzing activity on amylopeptin than starch, producing oligosaccharides from 4 to 5 glucose residues. Our findings show that P. brasiliensis Amy1p produces maltooligosaccharides which may act as a primer molecule for the fungal cell wall α-(1,3)-glucan biosynthesis by Ags1p.
authors.affiliation: Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela.
authors.forename: Emma
authors.initials: E
authors.lastname: Camacho
authors.forename: Victoria E
authors.initials: VE
authors.lastname: Sepulveda
authors.forename: William E
authors.initials: WE
authors.lastname: Goldman
authors.forename: Gioconda
authors.initials: G
authors.lastname: San-Blas
authors.forename: Gustavo A
authors.initials: GA
authors.lastname: Niño-Vega
mesh_terms: D000595:Amino Acid Sequence; D000687:Amylopectin / Q000378:metabolism; D002473:Cell Wall / Q000201:enzymology / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005816:Genetic Complementation Test; D005936:Glucans / Q000096:biosynthesis*; D006658:Histoplasma / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D009154:Mutation; D010228:Paracoccidioides / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D010802:Phylogeny; D016415:Sequence Alignment; D013213:Starch / Q000378:metabolism; D013379:Substrate Specificity; D014774:Virulence; D000516:alpha-Amylases / Q000235:genetics* / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D005936:Glucans; D013213:Starch; D000687:Amylopectin; D000516:alpha-Amylases