title: Streptococcus induces circulating CLA(+) memory T-cell-dependent epidermal cell activation in psoriasis.
abstract: Streptococcal throat infection is associated with a specific variant of psoriasis and with HLA-Cw6 expression. In this study, activation of circulating psoriatic cutaneous lymphocyte-associated antigen (CLA)(+) memory T cells cultured together with epidermal cells occurred only when streptococcal throat extracts were added. This triggered the production of Th1, Th17, and Th22 cytokines, as well as epidermal cell mediators (CXCL8, CXCL9, CXCL10, and CXCL11). Streptococcal extracts (SEs) did not induce any activation with either CLA(-) cells or memory T cells cultured together with epidermal cells from healthy subjects. Intradermal injection of activated culture supernatants into mouse skin induced epidermal hyperplasia. SEs also induced activation when we used epidermal cells from nonlesional skin of psoriatic patients with CLA(+) memory T cells. Significant correlations were found between SE induced upregulation of mRNA expression for ifn-γ, il-17, il-22, ip-10, and serum level of antistreptolysin O in psoriatic patients. This study demonstrates the direct involvement of streptococcal infection in pathological mechanisms of psoriasis, such as IL-17 production and epidermal cell activation.
authors.affiliation: Department of Dermatology, Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Ferran
authors.forename: Ana B
authors.initials: AB
authors.lastname: Galván
authors.forename: Catalina
authors.initials: C
authors.lastname: Rincón
authors.forename: Ester R
authors.initials: ER
authors.lastname: Romeu
authors.forename: Marc
authors.initials: M
authors.lastname: Sacrista
authors.forename: Erika
authors.initials: E
authors.lastname: Barboza
authors.forename: Ana
authors.initials: A
authors.lastname: Giménez-Arnau
authors.forename: Antonio
authors.initials: A
authors.lastname: Celada
authors.forename: Ramon M
authors.initials: RM
authors.lastname: Pujol
authors.forename: Luis F
authors.initials: LF
authors.lastname: Santamaria-Babí
mesh_terms: D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000097:blood / Q000276:immunology*; D000989:Antistreptolysin / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D054357:Chemokine CXCL10 / Q000235:genetics / Q000276:immunology; D003470:Culture Media / Q000378:metabolism; D000078404:Epidermal Cells; D004817:Epidermis / Q000276:immunology; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology*; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology; D016209:Interleukin-8 / Q000235:genetics / Q000276:immunology; D007378:Interleukins / Q000235:genetics / Q000276:immunology; D008562:Membrane Glycoproteins / Q000097:blood / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010612:Pharyngitis / Q000276:immunology; D011565:Psoriasis / Q000276:immunology* / Q000382:microbiology; D012333:RNA, Messenger / Q000378:metabolism; D013290:Streptococcal Infections / Q000276:immunology*; D013291:Streptococcus / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology; D000097043:Interleukin-22
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D000989:Antistreptolysin; D054357:Chemokine CXCL10; D003470:Culture Media; D007371:Interferon-gamma; D020381:Interleukin-17; D016209:Interleukin-8; D007378:Interleukins; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D000097043:Interleukin-22; C063419:CTAGE1 protein, human; C114753:CXCL10 protein, human; C485433:Cxcl10 protein, mouse