title: Dynamic alterations in the expression and localization of ACTL7a during capacitation in mouse spermatozoa.
abstract: OBJECTIVE
  To demonstrate that capacitation in mouse spermatozoa involves alterations in the expression and localization of ACTL7a.
  
  
  METHODS
  Determine the alteration in the expression level and localization of ACTL7a in the induction of capacitation in mouse spermatozoa.
  
  
  METHODS
  The Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, People's Republic of China.
  
  
  METHODS
  ICR (Institute of Cancer Research) mice.
  
  
  METHODS
  None.
  
  
  METHODS
  Western blot, indirect immunostaining.
  
  
  RESULTS
  The expression of ACTL7a is upregulated via the PKA pathway and undergoes remodeling during the early period of capacitation in mouse spermatozoa.
  
  
  CONCLUSIONS
  ACTL7a is an essential component of capacitation in mouse spermatozoa. The alteration in the expression and localization of ACTL7a may be the primary biochemical event in the induction of capacitation in mouse spermatozoa.
authors.affiliation: State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Tsinghua University, Beijing, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Fu
authors.forename: Wei
authors.initials: W
authors.lastname: Song
authors.forename: Shudong
authors.initials: S
authors.lastname: Zong
authors.forename: Samuel S
authors.initials: SS
authors.lastname: Koide
authors.forename: Shiying
authors.initials: S
authors.lastname: Miao
authors.forename: Linfang
authors.initials: L
authors.lastname: Wang
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology; D003994:Bucladesine / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D007546:Isoquinolines / Q000494:pharmacology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011493:Protein Kinase C / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D013075:Sperm Capacitation / Q000502:physiology*; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism*; D013449:Sulfonamides / Q000494:pharmacology
chemical_list: D000199:Actins; C483118:Actl7a protein, mouse; D001639:Bicarbonates; D007546:Isoquinolines; D047428:Protein Kinase Inhibitors; D013449:Sulfonamides; D003994:Bucladesine; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide; D002118:Calcium