title: Development and evaluation of reverse-transcription loop-mediated isothermal amplification for rapid detection of human immunodeficiency virus type 1.
abstract: OBJECTIVE
  The objective of this study was to establish a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid detection of human immunodeficiency virus type 1 (HIV-1).
  
  
  METHODS
  The HIV-1 integrase gene region was selected because it was a conserved part of the HIV-1 genome. Six primers specific to eight regions of the HIV-1 integrase gene were designed. A total of 171 samples (18 HIV-1 confirmed positive samples and 153 serum specimens were collected in this study) were tested by RT-LAMP and reverse-transcription polymerase chain reaction (RT-PCR). After amplification in an isothermal water bath for 45 min, samples containing HIV-1 generated the expected ladder-like products while other viruses generated no product.
  
  
  RESULTS
  The sensitivity and specificity of the RT-LAMP assay were evaluated by comparison with RT-PCR. The assay was significantly more sensitive than normal gel-based RT-PCR.
  
  
  CONCLUSIONS
  Because it is specific and simple, the RT-LAMP assay can be widely applied in clinical laboratories for rapid detection of HIV-1.
authors.affiliation: Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430073;  College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Xihong
authors.initials: X
authors.lastname: Zhao
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Chen
authors.forename: Youhong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaowei
authors.initials: X
authors.lastname: He
authors.forename: Wenmei
authors.initials: W
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.forename: Xingzhou
authors.initials: X
authors.lastname: Chen
authors.forename: Zhenbo
authors.initials: Z
authors.lastname: Xu
authors.forename: Nanjing
authors.initials: N
authors.lastname: Zhong
authors.forename: Guiyuan
authors.initials: G
authors.lastname: Ji
authors.forename: Liansheng
authors.initials: L
authors.lastname: Yang
authors.forename: Jihua
authors.initials: J
authors.lastname: Wang
mesh_terms: D017931:DNA Primers / Q000235:genetics; D015658:HIV Infections / Q000175:diagnosis* / Q000821:virology; D019427:HIV Integrase / Q000235:genetics; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013696:Temperature; D013997:Time Factors; D014773:Virology / Q000379:methods*
chemical_list: D017931:DNA Primers; D019427:HIV Integrase