title: Human gut flora-fermented nondigestible fraction from cooked bean ( Phaseolus vulgaris L.) modifies protein expression associated with apoptosis, cell cycle arrest, and proliferation in human adenocarcinoma colon cancer cells.
abstract: Metabolism of the nondigested fraction (NDF) from common bean ( Phaseolus vulgaris  L.) by the human gut flora (hgf) produces short-chain fatty acids (SCFAs) that may benefit cancer by reducing colorectal tumor risks. This paper reports the effect of fermentation products (FP) by hgf (FP-hgf) from NDF of cooked beans on survival and protein expression associated with apoptosis, cell cycle arrest, and proliferation in human adenocarcinoma colon cancer cells. FP-hgf was the only inoculum eliciting butyrate production after 24 h of NDF fermentation using different bacterial sources. FP-hgf inhibited HT-29 cell growth and modulated protein expression associated with apoptosis, cell cycle arrest, and proliferation, as well as morphological changes linked to apoptosis evaluated by TUNEL and hematoxylin and eosin stains, confirming previous results on gene expression. The current results suggest that fermentation of NDF from common beans can elicit beneficial chemoprotective effects in colon cancer by modulating protein expression in HT-29 cells.
authors.affiliation: Programa de Posgrado en Alimentos del Centro de la República (PROPAC), Research and Graduate Studies in Food Science, School of Chemistry, Querétaro State University , Qro 76010 Mexico.
authors.forename: Rocio
authors.initials: R
authors.lastname: Campos-Vega
authors.forename: Teresa
authors.initials: T
authors.lastname: García-Gasca
authors.forename: Ramón
authors.initials: R
authors.lastname: Guevara-Gonzalez
authors.forename: Minerva
authors.initials: M
authors.lastname: Ramos-Gomez
authors.forename: B Dave
authors.initials: BD
authors.lastname: Oomah
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Loarca-Piña
mesh_terms: D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000235:genetics; D059447:Cell Cycle Checkpoints / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D003106:Colon / Q000382:microbiology*; D003110:Colonic Neoplasms / Q000517:prevention & control; D005232:Fatty Acids, Volatile / Q000378:metabolism / Q000494:pharmacology*; D005285:Fermentation; D015870:Gene Expression / Q000187:drug effects; D019073:HT29 Cells; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D020287:In Situ Nick-End Labeling; D027805:Phaseolus / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*
chemical_list: D016588:Anticarcinogenic Agents; D005232:Fatty Acids, Volatile