title: Proteomic identification of camel seminal plasma: purification of β-nerve growth factor.
abstract: The camel seminal plasma contains a diverse array of components including lipids, carbohydrates, peptides, ions and proteins. These are essential for maintaining normal physiology of spermatozoa and are secreted mainly from the prostrate, epidydimis and bulbo-urethral glands of reproductive system. The protein profiles of camel seminal plasma were resolved by two-dimensional gel electrophoresis (2D-PAGE). The majority of the protein was found in acidic regions below pI 7.0 and the 19 brightly stained proteins were identified by MALDI-TOF/MS analysis. On the basis of proteomic profiles, β-nerve growth factor (β-NGF) was purified by ion-exchange and gel filtration chromatography and identified by SDS-PAGE and MALDI-TOF/MS analysis. It was further confirmed by western blotting experiments using rabbit anti-β-NGF primary antibody.
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kumar
authors.forename: Vinay Kumar
authors.initials: VK
authors.lastname: Sharma
authors.forename: Sudhuman
authors.initials: S
authors.lastname: Singh
authors.forename: Guru Rao
authors.initials: GR
authors.lastname: Hariprasad
authors.forename: Gorakh
authors.initials: G
authors.lastname: Mal
authors.forename: Alagiri
authors.initials: A
authors.lastname: Srinivasan
authors.forename: Savita
authors.initials: S
authors.lastname: Yadav
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods / Q000662:veterinary; D002162:Camelus / Q000235:genetics / Q000502:physiology*; D002850:Chromatography, Gel / Q000379:methods / Q000662:veterinary; D002852:Chromatography, Ion Exchange / Q000379:methods / Q000662:veterinary; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods / Q000662:veterinary; D008297:Male; D020932:Nerve Growth Factor / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D040901:Proteomics / Q000379:methods*; D012661:Semen / Q000502:physiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods / Q000662:veterinary
chemical_list: D020932:Nerve Growth Factor