title: Chlorhexidine does not increase immediate bond strength of etch-and-rinse adhesive to caries-affected dentin of primary and permanent teeth.
abstract: The aim of this study was to evaluate the effect of 2% chlorhexidine digluconate (CHX) on immediate bond strength of etch-and-rinse adhesive to sound (SD) and caries-affected (CAD) primary dentin compared with permanent dentin. Flat dentin surfaces from 20 primary molars (Pri) and 20 permanent molars (Perm) were assigned to 8 experimental groups (n=5) according to tooth type (Pri or Perm), dentin condition (SD or CAD - pH-cycling for 14 days) and treatment (control - C or 60 s application of 2% CHX solution after acid etching - CHX). The bonding system (Adper Single Bond 2) was applied according to manufacturer's instructions followed by resin composite application (Filtek Z250). After 24 h water storage, specimens with cross-section area of 0.8 mm² were prepared for being tested under microtensile test (1 mm/min). Data were submitted to ANOVA and Tukey's post hoc test (α=0.05). Failure mode was evaluated using a stereomicroscope at ×400. Treatment with CHX did not result in higher bond strength values than no pre-treatment (C groups), independently of tooth type. Primary teeth and caries-affected dentin showed significantly lower (p<0.05) bond strength means compared with permanent teeth and sound dentin, respectively. Predominance of adhesive/mixed failure was observed for all groups. CHX did not influence the immediate bond strength to sound or caries-affected dentin of primary and permanent teeth.
authors.affiliation: Department of Orthodontics and Pediatric Dentistry, University of São Paulo, São Paulo, SP, Brazil. tathilenzi@usp.br
authors.forename: Tathiane Larissa
authors.initials: TL
authors.lastname: Lenzi
authors.forename: Tamara Kerber
authors.initials: TK
authors.lastname: Tedesco
authors.forename: Fabio Zovico Maxnuck
authors.initials: FZ
authors.lastname: Soares
authors.forename: Alessandro Dourado
authors.initials: AD
authors.lastname: Loguercio
authors.forename: Rachel de Oliveira
authors.initials: Rde O
authors.lastname: Rocha
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D000268:Adhesiveness; D000891:Anti-Infective Agents, Local / Q000737:chemistry*; D002710:Chlorhexidine / Q000031:analogs & derivatives* / Q000737:chemistry; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding; D003731:Dental Caries / Q000473:pathology*; D003738:Dental Cements / Q000737:chemistry; D003799:Dental Stress Analysis / Q000295:instrumentation; D003804:Dentin / Q000648:ultrastructure*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D008963:Molar / Q000648:ultrastructure; D008964:Molar, Third / Q000648:ultrastructure; D010756:Phosphoric Acids / Q000737:chemistry; D013314:Stress, Mechanical; D013499:Surface Properties; D013718:Tensile Strength; D013997:Time Factors; D014094:Tooth, Deciduous / Q000648:ultrastructure*; D014867:Water / Q000737:chemistry
chemical_list: C554641:Adper single bond 2; D000891:Anti-Infective Agents, Local; D003188:Composite Resins; D003738:Dental Cements; D017220:Dentin-Bonding Agents; C444701:Filtek Z250; D010756:Phosphoric Acids; D014867:Water; C030242:phosphoric acid; C010882:chlorhexidine gluconate; D002710:Chlorhexidine