title: Comparative study on the inhibitory effect of caffeic and chlorogenic acids on key enzymes linked to Alzheimer's disease and some pro-oxidant induced oxidative stress in rats' brain-in vitro.
abstract: This study sought to investigate and compare the interaction of caffeic acid and chlorogenic acid on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), and some pro-oxidants (FeSO(4), sodium nitroprusside and quinolinic acid) induced oxidative stress in rat brain in vitro. The result revealed that caffeic acid and chlorogenic acid inhibited AChE and BChE activities in dose-dependent manner; however, caffeic acid had a higher inhibitory effect on AChE and BChE activities than chlorogenic acid. Combination of the phenolic acids inhibited AChE and BChE activities antagonistically. Furthermore, pro-oxidants such as, FeSO(4), sodium nitroprusside and quinolinic acid caused increase in the malondialdehyde (MDA) contents of the brain which was significantly decreased dose-dependently by the phenolic acids. Inhibition of AChE and BChE activities slows down acetylcholine and butyrylcholine breakdown in the brain. Therefore, one possible mechanism through which the phenolic acids exert their neuroprotective properties is by inhibiting AChE and BChE activities as well as preventing oxidative stress-induced neurodegeneration. However, esterification of caffeic acid with quinic acid producing chlorogenic acid affects these neuroprotective properties.
authors.affiliation: Functional Foods, Nutraceuticals and Phytomedicine Unit, Department of Biochemistry, Federal University of Technology, Akure, P.M.B. 704, 340001 Akure, Nigeria. goboh2001@yahoo.com
authors.forename: Ganiyu
authors.initials: G
authors.lastname: Oboh
authors.forename: Odunayo M
authors.initials: OM
authors.lastname: Agunloye
authors.forename: Ayodele J
authors.initials: AJ
authors.lastname: Akinyemi
authors.forename: Adedayo O
authors.initials: AO
authors.lastname: Ademiluyi
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Adefegha
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000544:Alzheimer Disease / Q000188:drug therapy / Q000201:enzymology*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D002091:Butyrylcholinesterase / Q000378:metabolism; D002109:Caffeic Acids / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D002726:Chlorogenic Acid / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D002109:Caffeic Acids; D002800:Cholinesterase Inhibitors; D017382:Reactive Oxygen Species; D002726:Chlorogenic Acid; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; C040048:caffeic acid