title: Anti-diabetic and anti-cataract effects of Chromolaena odorata Linn., in streptozotocin-induced diabetic rats.
abstract: BACKGROUND
  Chromolaena odorata Linn., is used in traditional Indian medicine in the treatment of diabetes and eye problems.
  
  
  OBJECTIVE
  The present study was designed to investigate the effect of the ethanol extract Chromolaena odorata leaves (ACO) in streptozotocin (STZ; 45 mg/kg, i.v) induced diabetes and cataract in rats.
  
  
  METHODS
  Different doses of ACO (200 and 400mg/kg) was administered once daily for eight weeks to STZ-induced diabetic rats. To know the mechanism of action of title plant, AUC(glucose), AUC(insulin), Homeostatic Model Assessment (HOMA), insulin tolerance test (ITT) and glucose uptake by rat hemi-diaphragms were carried out. Further, cataract score was taken once in a week upto eight weeks and opacity index was measured. HPLC fingerprinting profiling of ACO was also carried out.
  
  
  RESULTS
  Administration of ACO exhibited significant reduction in glucose, HOMA, lipid profiles and significantly improved glucose and insulin tolerance, glycogen content, glucose uptake by skeletal muscle, serum insulin and HDL-c levels. In addition, ACO also decreased oxidative stress by improving endogenous antioxidants. Further, treatment of ACO showed significantly reduced onset and extent of cataract.
  
  
  CONCLUSIONS
  The present data suggested that the treatment of ACO reversed the STZ-induced diabetes and cataract in rats. The observed beneficial effects may be mediated by interacting with multiple targets operating in diabetes mellitus and its complication. Taken together, this study provided the scientific evidence for the traditional use of Chromolaena odorata.
authors.affiliation: Sree Siddaganga College of Pharmacy, Tumkur, Karnataka 572 102, India.
authors.forename: M
authors.initials: M
authors.lastname: Onkaramurthy
authors.forename: V P
authors.initials: VP
authors.lastname: Veerapur
authors.forename: B S
authors.initials: BS
authors.lastname: Thippeswamy
authors.forename: T N Madhusudana
authors.initials: TN
authors.lastname: Reddy
authors.forename: Hunasagi
authors.initials: H
authors.lastname: Rayappa
authors.forename: S
authors.initials: S
authors.lastname: Badami
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002386:Cataract / Q000188:drug therapy*; D036604:Chromolaena / Q000737:chemistry*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D003964:Diaphragm / Q000187:drug effects / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry; D005260:Female; D005947:Glucose / Q000378:metabolism; D005951:Glucose Tolerance Test / Q000379:methods; D006003:Glycogen / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D007328:Insulin / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D007004:Hypoglycemic Agents; D007328:Insulin; D010936:Plant Extracts; D000431:Ethanol; D006003:Glycogen; D005947:Glucose