title: Immunohistochemical evaluation of CD25+ cell expression in the progression of periodontal disease.
abstract: It was assessed the immunohistochemical profile of CD25+ cells in cases of chronic gingivitis (CG) and chronic periodontitis (CP). Immunohistochemistry was carried out using streptoavidin-biotin complex and anti-CD25 antibody in 17 cases of CG and 25 cases of CP. Sixteen cases (94.1%) of CG were immunopositive. CD25 was focally expressed in 50% of the sample and diffusely expressed in 25%. The stained cells were localized not only beneath the epithelium, but also far from it. In relation to the cellular density quantification of CD25+ cells, score ++ was the most common. Concerning CP, all cases were immunopositive. CD25+ cells were expressed in focal or diffuse pattern either close or far from the epithelium. Diffuse distribution of positive cells throughout the connective tissue was seen in 60% of the cases and 32% showed focal or diffuse cellular pattern. Sixteen cases (64%) received score +++. It was identified that CD25+ cells are present in either a focal or a diffuse pattern in connective tissue. Significant differences in the density of cellular immunostaining between CG and CP were found. The greatest density was observed in CP cases, which suggests that the infiltrate of lymphocytes show a higher degree of cellular activation in periodontitis compared with gingivitis.
authors.affiliation: Dental School, State University of Paraíba, Campina Grande, PB, Brazil.
authors.forename: Ruthinéia Diogénes Alves Uchoa
authors.initials: RD
authors.lastname: Lins
authors.forename: Pollianna Muniz
authors.initials: PM
authors.lastname: Alves
authors.forename: Gustavo Pina
authors.initials: GP
authors.lastname: Godoy
authors.forename: Ericka Janine Dantas
authors.initials: EJ
authors.lastname: Silveira
authors.forename: Lélia Maria Guedes
authors.initials: LM
authors.lastname: Queiroz
authors.forename: Roseana de Almeida
authors.initials: Rde A
authors.lastname: Freitas
mesh_terms: D002452:Cell Count; D002908:Chronic Disease; D055113:Chronic Periodontitis / Q000276:immunology*; D003239:Connective Tissue Cells / Q000276:immunology; D018450:Disease Progression; D004847:Epithelial Cells / Q000276:immunology; D005347:Fibroblasts / Q000276:immunology; D005891:Gingivitis / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D053645:Interleukin-2 Receptor alpha Subunit / Q000032:analysis*; D008214:Lymphocytes / Q000276:immunology; D010950:Plasma Cells / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
chemical_list: C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit