title: Multiplexed surface micropatterning of proteins with a pressure-modulated microfluidic button-membrane.
abstract: We report the flow-based in situ patterning of multiple proteins in microfluidic channels by simply tuning the actuation pressure of a microfluidic button-membrane. We show that a single button-membrane can pattern several concentric protein annuli, and we apply this patterning approach to a multiplexed immunoassay.
authors.affiliation: Laboratory of Biological Network Characterization, Institute of Bioengineering, Ecole Polytechnique Federale de Lausanne, Station 17, CH-1015, Lausanne, Switzerland.
authors.forename: Jose L
authors.initials: JL
authors.lastname: Garcia-Cordero
authors.forename: Sebastian J
authors.initials: SJ
authors.lastname: Maerkl
mesh_terms: D000818:Animals; D002417:Cattle; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D055767:Immobilized Proteins / Q000737:chemistry*; D007118:Immunoassay; D008567:Membranes, Artificial; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D011312:Pressure; D013499:Surface Properties
chemical_list: D004129:Dimethylpolysiloxanes; D055767:Immobilized Proteins; D008567:Membranes, Artificial; C013830:baysilon