title: An application of high performance liquid chromatographic assay for the kinetic analysis of degradation of faropenem.
abstract: An isocratic RP-HPLC-DAD procedure was developed and validated for kinetic analysis of degradation of faropenem in bulk drug substance and in tablets. It involved the use of a C-18 analytical column (5 microm particle size, 250 mm x 4.6 mm), flow rate 1.3 ml/min and 50 microl injection volume. The mobile phase consisted of acetate buffer (pH 3.5) - acetonitrile (70:30 v/v). The determination was carried out at the wavelength of 323 nm. Kinetic studies of faropenem degradation in aqueous solutions included hydrolysis, oxidation, photolysis and thermal degradation. A derivative spectrophotometry was used as an alternative method to compare the observed rate constants.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Poznań, Poland. jpiontek@ump.edu.pl
authors.forename: J
authors.initials: J
authors.lastname: Cielecka-Piontek
authors.forename: A
authors.initials: A
authors.lastname: Krause
authors.forename: M
authors.initials: M
authors.lastname: Paczkowska
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004339:Drug Compounding; D004355:Drug Stability; D006851:Hydrochloric Acid; D007202:Indicators and Reagents; D007700:Kinetics; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet; D047090:beta-Lactams / Q000032:analysis* / Q000737:chemistry
chemical_list: D007202:Indicators and Reagents; D047090:beta-Lactams; C107057:fropenem; D006851:Hydrochloric Acid