title: Multiple Shoot Formation from Shoot Apex Cultures of Phaseolus vulgaris L.
abstract: Shoot apexes of 33 bean cultivars (Phaseolus vulgaris) were cultivated in order to explore possible genotypic differences pertaining to in vitro plant regeneration. This expiant source was found to be very promising during preliminary tests. Shoot apex cultures of these 33 cultivars were established on B5 medium in the presence of BA (0.5 μM). Callus, roots, and/or buds were found according to the genotype. Adventitious bud development varied according to the culture conditions (growth room or shaded greenhouse) and genotypes. This methodology was further explored with the cultivar Palmital Precoce whereby 276 buds were recovered from a 2,4-D x BA diallelic design with an average of 15 buds/shoot apex. Full plants of «Palmital Precoce» were raised following tertiary culture in the presence of GA3 and NAA.
authors.affiliation: Dept. Genetics, Institute of Agronomy, P.O. Box 28, Campinas, SP, Brazil.
authors.forename: I S
authors.initials: IS
authors.lastname: Martins
authors.forename: M R
authors.initials: MR
authors.lastname: Sondahl