title: RNA aptamers targeting cancer stem cell marker CD133.
abstract: The monoclonal antibody against the AC133 epitope of CD133 has been widely used as a cell surface marker of cancer stem cells in several different cancer types. Here, we describe the isolation and characterisation of two RNA aptamers, including the smallest described 15 nucleotide RNA aptamer, which specifically recognise the AC133 epitope and the CD133 protein with high sensitivity. As well, both these aptamers show superior tumour penetration and retention when compared to the AC133 antibody in a 3-D tumour sphere model. These novel CD133 aptamers will aid future development of cancer stem cell targeted therapeutics and molecular imaging.
authors.affiliation: School of Medicine, Deakin University, Pigdons Road, Waurn Ponds, Victoria 3217, Australia.
authors.forename: Sarah
authors.initials: S
authors.lastname: Shigdar
authors.forename: Liang
authors.initials: L
authors.lastname: Qiao
authors.forename: Shu-Feng
authors.initials: SF
authors.lastname: Zhou
authors.forename: Dongxi
authors.initials: D
authors.lastname: Xiang
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Lee Yong
authors.initials: LY
authors.lastname: Lim
authors.forename: Lingxue
authors.initials: L
authors.lastname: Kong
authors.forename: Lianhong
authors.initials: L
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Duan
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000235:genetics* / Q000276:immunology; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D045744:Cell Line, Tumor; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000235:genetics* / Q000276:immunology; D019073:HT29 Cells; D006801:Humans; D008969:Molecular Sequence Data; D014411:Neoplastic Stem Cells / Q000276:immunology* / Q000378:metabolism; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000235:genetics* / Q000276:immunology; D052156:SELEX Aptamer Technique / Q000379:methods
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D052157:Aptamers, Nucleotide; D000939:Epitopes; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides