title: Determining immunoassay cutoff value using Western blot results to predict hepatitis C infection in blood donors with low-titer anti-HCV reactivity.
abstract: Since the 1990s, blood donors have been scanned for anti-hepatitis C virus (anti-HCV) antibodies, which can be defined by enzyme immunoassay as a screening test. In this population, false-reactive ratios have been high. Recently, some authors have aimed to find a cutoff value for anti-HCV different from those established by test manufacturers to predict HCV infection. In this study, 321 patients, after two repeating tests, had reactive results in s/co <10 titers on anti-HCV test. The patients were 29.6 % (n = 95) in women and 70.4 % (n = 226) in men. The patients were classified into three groups by Western blot (WB) results (PS, positive; NG, negative; and ID, indeterminate). The average anti-HCV titer of the whole group was 2.61 ± 1.96. Anti-HCV titers of subgroups were 2.43 ± 1.95 in NG, 4.93 ± 2.53 in PS, and 2.50 ± 1.65 in ID (p < 0.001). There was a significant difference between NG and PS and between PS and ID subgroups (p < 0.001). There was a positive correlation between WB and anti-HCV titers in all patients (r = 0.298, p < 0.001), in women (r = 0.282, p < 0.001), and in men (r = 0.337, p = 0.002). According to receiver operator characteristic curve analysis, the cutoff value of anti-HCV titer to predict hepatitis C infection was >2.61 s/co, with 74.1 % sensitivity and 71.6 % specificity (area under the curve, 0.820; 95 % confidence interval, 0.753 to 0.887). We suggest that an effective cutoff value for anti-HCV other than that established by the manufacturer cannot be assigned to predict hepatitis C infection for blood donors in low-prevalence areas.
authors.affiliation: Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Abant Izzet Baysal University, Golkoy, Bolu, Turkey. abdulbayrak@yahoo.com
authors.forename: Abdulkadir
authors.initials: A
authors.lastname: Kucukbayrak
authors.forename: Saadet
authors.initials: S
authors.lastname: Cakmak
authors.forename: Ismail Necati
authors.initials: IN
authors.lastname: Hakyemez
authors.forename: Tekin
authors.initials: T
authors.lastname: Tas
authors.forename: Hayrettin
authors.initials: H
authors.lastname: Akdeniz
mesh_terms: D015153:Blotting, Western / Q000379:methods*; D019411:Clinical Laboratory Techniques / Q000379:methods* / Q000592:standards*; D016174:Hepacivirus / Q000276:immunology*; D018937:Hepatitis C Antibodies / Q000097:blood*; D019698:Hepatitis C, Chronic / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D018937:Hepatitis C Antibodies