title: Nuclear microsatellite primers for the endangered relict fir, Abies pinsapo (Pinaceae) and cross-amplification in related Mediterranean Species.
abstract: Twelve nuclear microsatellite primers (nSSR) were developed for the endangered species Abies pinsapo Boiss. to enable the study of gene flow and genetic structure in the remaining distribution areas. Microsatellite primers were developed using next-generation sequencing (454) data from a single Abies pinsapo individual. Primers were applied to thirty individuals from the three extant localities. The number of alleles per locus ranged from one to four. Cross-amplification was tested for other Abies species from the Mediterranean Basin, and most of the loci showed higher polymorphisms in the Mediterranean species than in A. pinsapo. These microsatellite markers provide tools for conservation genetic studies in Abies pinsapo as well other Abies species from the Mediterranean Basin.
authors.affiliation: Departamento de Biología Vegetal y Ecología, Universidad de Sevilla, Apdo. 1095, Sevilla E-41080, Spain. jsanchez15@us.es.
authors.forename: Jose M
authors.initials: JM
authors.lastname: Sánchez-Robles
authors.forename: Francisco
authors.initials: F
authors.lastname: Balao
authors.forename: Juan L
authors.initials: JL
authors.lastname: García-Castaño
authors.forename: Anass
authors.initials: A
authors.lastname: Terrab
authors.forename: Laura
authors.initials: L
authors.lastname: Navarro-Sampedro
authors.forename: Salvador
authors.initials: S
authors.lastname: Talavera
mesh_terms: D028202:Abies / Q000235:genetics*; D018744:DNA, Plant; D056727:Endangered Species; D051456:Gene Flow; D056426:Genetic Loci; D019083:Mediterranean Region; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D018744:DNA, Plant