title: Determination of psilocin, bufotenine, LSD and its metabolites in serum, plasma and urine by SPE-LC-MS/MS.
abstract: A validated method for the simultaneous determination of psilocin, bufotenine, lysergic acid diethylamide and its metabolites in serum, plasma and urine using liquid chromatography-electrospray ionization/tandem mass spectrometry was developed. During the solid-phase extraction procedure with polymeric mixed-mode cation exchange columns, the unstable analytes were protected by ascorbic acid, drying with nitrogen and exclusion of light. The limits of detection and quantitation for all analytes were low. Recovery was ≥86 % for all analytes and no significant matrix effects were observed. Interday and intraday imprecisions at different concentrations ranged from 1.1 to 8.2 % relative standard deviation, bias was within ±5.3 %. Processed samples were stable in the autosampler for at least 2 days. Furthermore, freeze/thaw and long-term stability were investigated. The method was successfully applied to authentic serum and urine samples.
authors.affiliation: Institute of Legal Medicine, University Hospital Münster, Röntgenstr. 23, 48149 Münster, Germany. rafaela.martin@ukmuenster.de
authors.forename: Rafaela
authors.initials: R
authors.lastname: Martin
authors.forename: Jennifer
authors.initials: J
authors.lastname: Schürenkamp
authors.forename: Angela
authors.initials: A
authors.lastname: Gasse
authors.forename: Heidi
authors.initials: H
authors.lastname: Pfeiffer
authors.forename: Helga
authors.initials: H
authors.lastname: Köhler
mesh_terms: D000328:Adult; D002027:Bufotenin / Q000032:analysis* / Q000097:blood / Q000652:urine; D016022:Case-Control Studies; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D053593:Forensic Toxicology; D006213:Hallucinogens / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D008238:Lysergic Acid Diethylamide / Q000032:analysis* / Q000097:blood / Q000652:urine; D008297:Male; D008875:Middle Aged; D011562:Psilocybin / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000652:urine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016037:Single-Blind Method; D052616:Solid Phase Extraction / Q000379:methods*; D013048:Specimen Handling; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D006213:Hallucinogens; D002027:Bufotenin; D011562:Psilocybin; D008238:Lysergic Acid Diethylamide; C009105:psilocin