title: Technical considerations for reduced representation bisulfite sequencing with multiplexed libraries.
abstract: Reduced representation bisulfite sequencing (RRBS), which couples bisulfite conversion and next generation sequencing, is an innovative method that specifically enriches genomic regions with a high density of potential methylation sites and enables investigation of DNA methylation at single-nucleotide resolution. Recent advances in the Illumina DNA sample preparation protocol and sequencing technology have vastly improved sequencing throughput capacity. Although the new Illumina technology is now widely used, the unique challenges associated with multiplexed RRBS libraries on this platform have not been previously described. We have made modifications to the RRBS library preparation protocol to sequence multiplexed libraries on a single flow cell lane of the Illumina HiSeq 2000. Furthermore, our analysis incorporates a bioinformatics pipeline specifically designed to process bisulfite-converted sequencing reads and evaluate the output and quality of the sequencing data generated from the multiplexed libraries. We obtained an average of 42 million paired-end reads per sample for each flow-cell lane, with a high unique mapping efficiency to the reference human genome. Here we provide a roadmap of modifications, strategies, and trouble shooting approaches we implemented to optimize sequencing of multiplexed libraries on an a RRBS background.
authors.affiliation: Department of Pathology, Dunedin School of Medicine, University of Otago, 270 Great King Street, Dunedin 9054, New Zealand.
authors.forename: Aniruddha
authors.initials: A
authors.lastname: Chatterjee
authors.forename: Euan J
authors.initials: EJ
authors.lastname: Rodger
authors.forename: Peter A
authors.initials: PA
authors.lastname: Stockwell
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Weeks
authors.forename: Ian M
authors.initials: IM
authors.lastname: Morison
mesh_terms: D020029:Base Pairing / Q000235:genetics; D030541:Databases, Genetic; D015723:Gene Library; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods* / Q000592:standards; D013447:Sulfites / Q000737:chemistry*
chemical_list: D013447:Sulfites; C042345:hydrogen sulfite