title: Daintain/AIF-1 (allograft inflammatory factor-1) promotes erythrocyte lysis and heme release probably via binding to hemoglobin.
abstract: Free heme is potentially cytotoxic, particularly in the presence of oxidants or activated phagocytes. Daintain/AIF-1 (allograft inflammatory factor-1) is a macrophage factor that has been implicated in the regulation of inflammation. In the present study, daintain/AIF-1 was found to induce cytolysis of erythrocytes, resulting in heme release in vitro. Furthermore, the interacting protein of daintain/AIF-1 was purified by daintain/AIF-1-6 histidine antigen fusion protein nickel affinity chromatography. MALDI-TOF-MS analysis identified hemoglobin subunit beta-1 as an interacting protein of daintain/AIF-1.These data suggest that daintain/AIF-1 may be involved in heme-associated diseases.
authors.affiliation: College of Life Science and Technology, Southwest University for Nationalities, 610041, Chengdu, China.
authors.forename: Yan-Ying
authors.initials: YY
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Dong-Jing
authors.initials: DJ
authors.lastname: Yan
authors.forename: Zheng-Wang
authors.initials: ZW
authors.lastname: Chen
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000502:physiology*; D002846:Chromatography, Affinity; D004912:Erythrocytes / Q000378:metabolism*; D006418:Heme / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism*; D051379:Mice; D008840:Microfilament Proteins / Q000502:physiology*; D011485:Protein Binding; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: C491049:Aif1 protein, mouse; D002135:Calcium-Binding Proteins; D006454:Hemoglobins; D008840:Microfilament Proteins; D006418:Heme