title: IL-1RN +2018T>C polymorphism is correlated with colorectal cancer.
abstract: Epidemiological and experimental evidence indicates chronic inflammation as a risk factor for colorectal cancer. We investigated whether IL-1B -511C>T (rs16944), IL-1B +3954C>T (rs1143634) and IL1-RN +2018T>C (rs419598) cytokine polymorphisms are correlated with colorectal cancer. Blood samples were obtained from 377 Romanian subjects: 144 patients with sporadic colorectal cancer and 233 healthy controls. Polymorphisms were analyzed by allelic discrimination TaqMan PCR assays with specific probes. The results of our study showed that IL-1RN +2018T>C polymorphism is associated with colorectal cancer. We found that there was a significant difference in the frequency of CC genotype between patients with colorectal cancer and the control group (OR 2.42, 95 % CI: 1.06-5.53, p = 0,034) when TT genotype was used as reference. Furthermore, in a stratified analysis, a positive association was found only for IL-1RN +2018CC genotype, that was limited to early I and II stages (OR 2.72, 95 % CI: 1.05-7.03, p = 0,033). We did not find any association between any of the IL-1B polymorphisms and colorectal cancer. In conclusion this study found that IL-1RN +2018T>C polymorphism is associated with colorectal cancer, mainly for localized disease.
authors.affiliation: Research Center of Gastroenterology and Hepatology and Human Genomic Laboratory, University of Medicine and Pharmacy of Craiova, 1 May Street No 66, Craiova, Romania. buradaflorin@gmail.com
authors.forename: Florin
authors.initials: F
authors.lastname: Burada
authors.forename: Theodor
authors.initials: T
authors.lastname: Dumitrescu
authors.forename: Raluca
authors.initials: R
authors.lastname: Nicoli
authors.forename: Marius Eugen
authors.initials: ME
authors.lastname: Ciurea
authors.forename: Cristina
authors.initials: C
authors.lastname: Angelescu
authors.forename: Francisc
authors.initials: F
authors.lastname: Mixich
authors.forename: Mihai
authors.initials: M
authors.lastname: Ioana
mesh_terms: D000368:Aged; D000483:Alleles; D015179:Colorectal Neoplasms / Q000097:blood / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D007249:Inflammation / Q000235:genetics*; D053590:Interleukin 1 Receptor Antagonist Protein / Q000097:blood / Q000235:genetics*; D053583:Interleukin-1beta / Q000097:blood / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D012307:Risk Factors
chemical_list: C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D053583:Interleukin-1beta