title: Adherence of Brachyspira hyodysenteriae to porcine intestinal epithelial cells is inhibited by antibodies against outer membrane proteins.
abstract: Attachment of Brachyspira hyodysenteriae to intestinal epithelial cell lines and its possible mediation by outer membrane proteins (OMPs) of the spirochete were examined. Different B. hyodysenteriae serotypes were shown to adhere to rat and swine intestinal epithelial cells (IEC-18 and IPEC-J2) in vitro but not to the human rectal tumor cell line (HRT-18). Adherence of strain B204 to IPEC-J2 cells was reduced by rOMP-specific antisera in amounts of 29 % (anti-rBhlp29.7), 59 % (anti-rBhlp16), 70 % (anti-rBhmp39h), and 74 % (anti-rBhmp39h), respectively. By use of pooled antisera against Bhlp16 and Bhmp39f inhibition rates of the other serotypes ranged from 53 to 91 %. In a western blot assay OMPs of all serotypes but one were detected by the respective rOMP antisera. Altogether the results indicated that OMPs of B. hyodysenteriae displayed a serotype overlapping antigenicity and mediated adherence of the spirochetes to animal cell cultures.
authors.affiliation: Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig Universität Gießen, Frankfurter Straße 85-89, 35392, Gießen, Germany.
authors.forename: Maike
authors.initials: M
authors.lastname: Gömmel
authors.forename: Stefanie
authors.initials: S
authors.lastname: Barth
authors.forename: Carsten
authors.initials: C
authors.lastname: Heydel
authors.forename: Georg
authors.initials: G
authors.lastname: Baljer
authors.forename: Werner
authors.initials: W
authors.lastname: Herbst
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology / Q000494:pharmacology*; D000918:Antibody Specificity / Q000276:immunology; D001422:Bacterial Adhesion / Q000187:drug effects* / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D017401:Brachyspira hyodysenteriae / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D003001:Cloning, Molecular; D004847:Epithelial Cells / Q000382:microbiology*; D015870:Gene Expression; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D011994:Recombinant Proteins