title: Painting proteins blue: β-(1-azulenyl)-L-alanine as a probe for studying protein-protein interactions.
abstract: We demonstrated that β-(1-azulenyl)-L-alanine, a fluorescent pseudoisosteric analog of tryptophan, exhibits weak environmental dependence and thus allows for using weak intrinsic quenchers, such as methionines, to monitor protein-protein interactions while not perturbing them.
authors.affiliation: Department of Chemistry, Syracuse University, 111 College Place, Syracuse, NY 13244, USA.
authors.forename: Yurii S
authors.initials: YS
authors.lastname: Moroz
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Binder
authors.forename: Patrik
authors.initials: P
authors.lastname: Nygren
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Caputo
authors.forename: Ivan V
authors.initials: IV
authors.lastname: Korendovych
mesh_terms: D002147:Calmodulin / Q000737:chemistry / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D008715:Methionine / Q000737:chemistry; D009219:Myosin-Light-Chain Kinase / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D014364:Tryptophan / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002147:Calmodulin; D005456:Fluorescent Dyes; D011506:Proteins; D014364:Tryptophan; D008715:Methionine; D009219:Myosin-Light-Chain Kinase