title: A chemically functionalized magnetic nanoplatform for rapid and specific biomolecular recognition and separation.
abstract: We have developed a target-molecule-functionalized magnetic nanoparticle (MNP)-based method to facilitate the study of biomolecular recognition and separation. The superparamagnetic property of MNPs allows the corresponding biomolecules to be rapidly separated from crude biofluids with a significant improvement in recovery yield and specificity. Various MNPs functionalized with tag molecules (chitin, heparin, and amylose) were synthesized for recombinant protein purification, and several probe-functionalized MNPs, such as nitrilotriacetic acid (NTA)@MNP and P(k)@MNP, exhibited excellent extraction efficiency for proteins. In a cell recognition study, mannose-functionalized MNPs allowed specific purification of  Escherichia coli  with FimH adhesin on the surface. In an immunoprecipitation assay, the antibody-conjugated MNPs reduced the incubation time from 12 to 1 h while maintaining a comparable efficiency. The functionalized MNPs were also used in a membrane proteomic study that utilized the interaction between streptavidin-functionalized MNPs and biotinylated cell membrane proteins. Overall, the functionalized MNPs were demonstrated to be promising probes for the specific separation of targets from proteins to cells and proteomics.
authors.affiliation: Department of Chemistry, National Sun Yat-sen University, 70, Lienhai Road, Kaohsiung 80424, Taiwan. pclin@mail.nsysu.edu.tw
authors.forename: Po-Chiao
authors.initials: PC
authors.lastname: Lin
authors.forename: Ching-Ching
authors.initials: CC
authors.lastname: Yu
authors.forename: Huan-Ting
authors.initials: HT
authors.lastname: Wu
authors.forename: Ying-Wei
authors.initials: YW
authors.lastname: Lu
authors.forename: Chia-Li
authors.initials: CL
authors.lastname: Han
authors.forename: An-Kai
authors.initials: AK
authors.lastname: Su
authors.forename: Yu-Ju
authors.initials: YJ
authors.lastname: Chen
authors.forename: Chun-Cheng
authors.initials: CC
authors.lastname: Lin
mesh_terms: D001665:Binding Sites / Q000502:physiology; D002686:Chitin / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D060328:Magnetic Phenomena; D053758:Nanoparticles / Q000032:analysis / Q000737:chemistry*; D011485:Protein Binding / Q000502:physiology; D013997:Time Factors
chemical_list: D002686:Chitin