title: Establishment of a conditional transgenic mouse model expressing human uncoupling protein 2 in vascular smooth muscle cells.
abstract: Increased oxidative stress is involved in the development of vascular dysfunction and remodeling. Uncoupling protein 2 (UCP2) regulates the production of reactive oxygen species in vascular smooth muscle cells (SMCs). To promote the study of the role of UCP2 in vascular diseases, a transgenic mouse model expressing human UCP2 (hUCP2) in vascular SMCs was established. We constructed a plasmid carrying the 2.3 kb rabbit smooth muscle myosin heavy chain promoter and the hUCP2 gene. We used this plasmid to produce transgenic mice by pro-nuclear microinjection. Six offspring were identified as founder mice that were used to establish a transgenic mouse lineage. The transgenic mice showed a significant increase in hUCP mRNA expression in the aorta. Moreover, hUCP2 overexpression inhibited the production of superoxide and increased the bioavailability of nitric oxide (NO). In this study, we established a hUCP2 transgenic mouse model, which will enable further studies on the role of UCP2 in vascular dysfunction and remodeling.
authors.affiliation: Department of Cardiology, General Hospital of PLA Chengdu Military Area Command, Chengdu, Sichuan 610083,  P.R. China.
authors.forename: Shuangtao
authors.initials: S
authors.lastname: Ma
authors.forename: DE
authors.initials: D
authors.lastname: Li
authors.forename: Dachun
authors.initials: D
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Tan
authors.forename: Bing
authors.initials: B
authors.lastname: Tang
authors.forename: Feipeng
authors.initials: F
authors.lastname: Jin
authors.forename: Sihua
authors.initials: S
authors.lastname: Jiang
authors.forename: Xiuchuan
authors.initials: X
authors.lastname: Li
authors.forename: Yongjian
authors.initials: Y
authors.lastname: Yang