title: Effect of Kupffer cells on immune tolerance in liver transplantation.
abstract: OBJECTIVE
  To observe the effect of Kupffer cells on immune tolerance in liver transplantation.
  
  
  METHODS
  The rats were randomly divided into A, B and C groups. A group was sham operation group. The donor rats of group B had intraperitoneal injection of 1 nmol Kuppffer cells every other day for three days before liver transplantation. Rats of group C were injected with equal saline. The rat liver transplantation models were established by modified Kamada's two-cuff technique. The rats were sacrificed after 24 hours. The concentrations of ALT and AST in serum were measured with the biochemical analyzer. The level of IL-2 and TNF-α in serum were measured by ELISA method. The apoptotic indexes were detected by immunohistochemical assay.
  
  
  RESULTS
  The concentration of ALT, AST, IL-1 and TNF-α in A, B and C groups were increased successively. The levels of group C were significantly higher than that of group B and A (P<0.05), and the levels of group B were significantly higher than that of group A (P<0.05). The apoptotic indexes of three groups were 3.40±0.37, 14.70±2.54 and 26.33±3.65, respectively, with significant difference among three groups (P<0.05).
  
  
  CONCLUSIONS
  Pretreatment with Kupffer cells can reduce liver injury and raise liver transplantation immune tolerance.
authors.affiliation: Second Xiangya Hospital of Central South University, Changsha 410011, China.
authors.forename: Guang-Shun
authors.initials: GS
authors.lastname: Chen
authors.forename: Hai-Zhi
authors.initials: HZ
authors.lastname: Qi
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001219:Aspartate Aminotransferases / Q000097:blood; D016009:Chi-Square Distribution; D007108:Immune Tolerance; D007375:Interleukin-1 / Q000097:blood; D007728:Kupffer Cells / Q000276:immunology* / Q000637:transplantation*; D008099:Liver / Q000737:chemistry / Q000276:immunology / Q000473:pathology; D016031:Liver Transplantation / Q000276:immunology* / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase