title: Establishment of an in vitro system representing the chicken gut-associated lymphoid tissue.
abstract: The bursa of Fabricius is critical for B cell development and differentiation in chick embryos. This study describes the production in vitro, from dissociated cell suspensions, of cellular agglomerates with functional similarities to the chicken bursa. Co-cultivation of epithelial and lymphoid cells obtained from embryos at the appropriate developmental stage regularly led to agglomerate formation within 48 hours. These agglomerates resembled bursal tissue in having lymphoid clusters overlaid by well organized epithelium. Whereas lymphocytes within agglomerates were predominantly Bu-1a(+), a majority of those emigrating onto the supporting membrane were Bu-1a(-) and IgM(+). Both agglomerates and emigrant cells expressed activation-induced deaminase with levels increasing after 24 hours. Emigrating cells were actively proliferating at a rate in excess of both the starting cell population and the population of cells remaining in agglomerates. The potential usefulness of this system for investigating the response of bursal tissue to avian Newcastle disease virus (strain AF2240) was examined.
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Noorjahan Banu
authors.initials: NB
authors.lastname: Alitheen
authors.forename: Susan Jane
authors.initials: SJ
authors.lastname: McClure
authors.forename: Swee Keong
authors.initials: SK
authors.lastname: Yeap
authors.forename: Ye Wen
authors.initials: YW
authors.lastname: Kristeen-Teo
authors.forename: Sheau Wei
authors.initials: SW
authors.lastname: Tan
authors.forename: Peter
authors.initials: P
authors.lastname: McCullagh
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology; D002060:Bursa of Fabricius / Q000166:cytology* / Q000648:ultrastructure; D002449:Cell Aggregation; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens / Q000033:anatomy & histology*; D003564:Cytidine Deaminase / Q000235:genetics / Q000378:metabolism; D004847:Epithelial Cells / Q000473:pathology / Q000648:ultrastructure / Q000821:virology; D004848:Epithelium / Q000033:anatomy & histology; D005452:Fluoresceins / Q000378:metabolism; D005453:Fluorescence; D041981:Gastrointestinal Tract / Q000166:cytology*; D019394:Ki-67 Antigen / Q000378:metabolism; D009521:Newcastle Disease / Q000473:pathology / Q000821:virology; D009522:Newcastle disease virus / Q000648:ultrastructure; D009195:Peroxidase / Q000378:metabolism; D010641:Phenotype; D060888:Real-Time Polymerase Chain Reaction; D013154:Spleen / Q000166:cytology; D013194:Staining and Labeling; D013388:Succinimides / Q000378:metabolism; D000098830:AICDA (Activation-Induced Cytidine Deaminase)
chemical_list: C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D005452:Fluoresceins; D019394:Ki-67 Antigen; D013388:Succinimides; D009195:Peroxidase; D000098830:AICDA (Activation-Induced Cytidine Deaminase); D003564:Cytidine Deaminase