title: [Over-expressed MiR-103 promotes porcine adipocyte differentiation].
abstract: To clarify the function of miR-103 in the differentiation of porcine preadipocyte, we carried out real-time PCR to detect the expression pattern of miR-103 during adipogenesis, and clarified its expression tendency through cell differentiation. Then we used adenovirus that overexpressed miR-103 to infect porcine preadipocyte. Subsequently, mRNA and protein expression of adipogenesis marker--PPARgamma and aP2 was analyzed by real-time PCR and Western blotting. At last, Oil-Red O staining was used to detect lipids accumulation in the 8th day after adipogenic inducement. The expression of miR-103 increased during adipocyte differentiation; compared with the control, the preadipocyte infected by pAd-miR-103 had an elevated expression level of adipocyte marker gene PPARgamma, aP2, and obvious lipid droplet was seen in the 8th day after adipogenic inducement. These results showed that miR-103 can enhance adipogenesis in primary cultured porcine adipocytes.
authors.affiliation: Laboratory of Animal Fat Deposition and Muscle Development, Northwest A & F University, Yangling 712100, Shaanxi, China.
authors.forename: Meihang
authors.initials: M
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Qiu
authors.forename: Shuai
authors.initials: S
authors.lastname: Liu
authors.forename: Peiyue
authors.initials: P
authors.lastname: Dong
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Ning
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Li
authors.forename: Gongshe
authors.initials: G
authors.lastname: Yang
authors.forename: Shiduo
authors.initials: S
authors.lastname: Sun
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D061251:Primary Cell Culture; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013552:Swine; D014162:Transfection
chemical_list: D035683:MicroRNAs; D047495:PPAR gamma; D012333:RNA, Messenger