title: Single mechanosensitive and Ca²⁺-sensitive channel currents recorded from mouse and human embryonic stem cells.
abstract: Cell-attached and inside-out patch clamp recording was used to compare the functional expression of membrane ion channels in mouse and human embryonic stem cells (ESCs). Both ESCs express mechanosensitive Ca²⁺ permeant cation channels (MscCa) and large conductance (200 pS) Ca²⁺-sensitive K⁺ (BK(Ca²⁺)) channels but with markedly different patch densities. MscCa is expressed at higher density in mESCs compared with hESCs (70 % vs. 3 % of patches), whereas the BK(Ca²⁺) channel is more highly expressed in hESCs compared with mESCs (~50 % vs. 1 % of patches). ESCs of both species express a smaller conductance (25 pS) nonselective cation channel that is activated upon inside-out patch formation but is neither mechanosensitive nor strictly Ca²⁺-dependent. The finding that mouse and human ESCs express different channels that sense membrane tension and intracellular [Ca²⁺] may contribute to their different patterns of growth and differentiation in response to mechanical and chemical cues.
authors.affiliation: Department of Stem Cells, Andalusian Center for Molecular Biology and Regenerative Medicine, Seville, Spain.
authors.forename: Bernat
authors.initials: B
authors.lastname: Soria
authors.forename: Sergio
authors.initials: S
authors.lastname: Navas
authors.forename: Abdelkrim
authors.initials: A
authors.lastname: Hmadcha
authors.forename: Owen P
authors.initials: OP
authors.lastname: Hamill
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000378:metabolism*; D053595:Embryonic Stem Cells / Q000502:physiology*; D006801:Humans; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D018408:Patch-Clamp Techniques
chemical_list: D015220:Calcium Channels