title: Plasmodium falciparum Na+/H+ exchanger (pfnhe-1) genetic polymorphism in Indian Ocean malaria-endemic areas.
abstract: To date, 11 studies conducted in different countries to test the association between Plasmodium falciparum Na(+)/H(+) exchanger gene (pfnhe-1; PF13_0019) polymorphisms and in vitro susceptibility to quinine have generated conflicting data. In this context and to extend our knowledge of the genetic polymorphism of Pfnhe gene, we have sequenced the ms4760 locus from 595 isolates collected in the Comoros (N = 250; an area with a high prevalence of chloroquine and sulfadoxine-pyrimethamine resistance) and Madagascar (N = 345; a low drug-resistance area). Among them, 29 different alleles were observed, including 8 (27%) alleles not previously described. Isolates from the Comoros showed more repeats in block II (DNNND), which some studies have found to be positively associated with in vitro resistance to quinine, compared with isolates from Madagascar. Additional studies are required to better define the mechanisms underlying quinine resistance, which involve multiple gene interactions.
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Hôpital Avicenne, AP-HP, Bobigny, France. landyvalerie@gmail.com
authors.forename: Valérie
authors.initials: V
authors.lastname: Andriantsoanirina
authors.forename: Nimol
authors.initials: N
authors.lastname: Khim
authors.forename: Arsene
authors.initials: A
authors.lastname: Ratsimbasoa
authors.forename: Benoit
authors.initials: B
authors.lastname: Witkowski
authors.forename: Christophe
authors.initials: C
authors.lastname: Benedet
authors.forename: Lydie
authors.initials: L
authors.lastname: Canier
authors.forename: Christiane
authors.initials: C
authors.lastname: Bouchier
authors.forename: Magali
authors.initials: M
authors.lastname: Tichit
authors.forename: Rémy
authors.initials: R
authors.lastname: Durand
authors.forename: Didier
authors.initials: D
authors.lastname: Ménard
mesh_terms: D000818:Animals; D019353:Endemic Diseases; D006801:Humans; D019156:Indian Ocean; D010963:Plasmodium falciparum / Q000235:genetics*; D011110:Polymorphism, Genetic; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics*
chemical_list: D017923:Sodium-Hydrogen Exchangers