title: Label-free electrochemical immunosensor for prostate-specific antigen based on silver hybridized mesoporous silica nanoparticles.
abstract: Due to high sensitivity and good selectivity, electrochemical immunosensor is often used to detect tumor markers. Prostate-specific antigen (PSA) is the most validated tumor marker for prostate cancer. In this work, mesoporous silica nanoparticles (MSNs) were used to increase the fixation capacity of primary antibody, and silver nanoparticles (Ag NPs) were used to enhance the electron transfer rates. Silver hybridized mesoporous silica nanoparticles (Ag@MSNs) were synthesized and used as electrode material. Hydroquinone (HQ) generated a stable electrochemical signal and was used as a mediator. Based on the specific antibody-antigen interaction, a label-free immunosensor was developed for the sensing of PSA. The current method allows us to detect PSA over a wide concentration range from 0.05 to 50.0 ng ml(-1) with a detection limit of 15 pg ml(-1). The proposed immunosensor was used to determine PSA in human serum with satisfactory results.
authors.affiliation: Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Haiqin
authors.initials: H
authors.lastname: Yu
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.forename: Hongmin
authors.initials: H
authors.lastname: Ma
authors.forename: He
authors.initials: H
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Du
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
mesh_terms: D000906:Antibodies / Q000276:immunology; D055664:Electrochemical Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006873:Hydroquinones / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D016062:Porosity; D017430:Prostate-Specific Antigen / Q000097:blood*; D012822:Silicon Dioxide / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*
chemical_list: D000906:Antibodies; D006873:Hydroquinones; D012834:Silver; D012822:Silicon Dioxide; D017430:Prostate-Specific Antigen; C031927:hydroquinone