title: Bone-specific overexpression of NPY modulates osteogenesis.
abstract: OBJECTIVE
  Neuropeptide Y (NPY) is a peptide involved in the regulation of appetite and energy homeostasis. Genetic data indicates that NPY decreases bone formation via central and peripheral activities. NPY is produced by various cell types including osteocytes and osteoblasts and there is evidence suggesting that peripheral NPY is important for regulation of bone formation. We sought to investigate the role of bone-derived NPY in bone metabolism.
  
  
  METHODS
  We generated a mouse where NPY was over-expressed specifically in mature osteoblasts and osteocytes (Col2.3NPY) and characterized the bone phenotype of these mice in vivo and in vitro.
  
  
  RESULTS
  Trabecular and cortical bone volume was reduced in 3-month-old animals, however bone formation rate and osteoclast activity were not significantly changed. Calvarial osteoblast cultures from Col2.3NPY mice also showed reduced mineralization and expression of osteogenic marker genes.
  
  
  CONCLUSIONS
  Our data suggest that osteoblast/osteocyte-derived NPY is capable of altering osteogenesis in vivo and in vitro and may represent an important source of NPY for regulation of bone formation. However, it is possible that other peripheral sources of NPY such as the sympathetic nervous system and vasculature also contribute to peripheral regulation of bone turnover.
authors.affiliation: Department of Reconstructive Sciences, University of Connecticut Health Center, Farmington, CT 06032, USA.
authors.forename: I
authors.initials: I
authors.lastname: Matic
authors.forename: B G
authors.initials: BG
authors.lastname: Matthews
authors.forename: T
authors.initials: T
authors.lastname: Kizivat
authors.forename: J C
authors.initials: JC
authors.lastname: Igwe
authors.forename: I
authors.initials: I
authors.lastname: Marijanovic
authors.forename: S T
authors.initials: ST
authors.lastname: Ruohonen
authors.forename: E
authors.initials: E
authors.lastname: Savontaus
authors.forename: D J
authors.initials: DJ
authors.lastname: Adams
authors.forename: I
authors.initials: I
authors.lastname: Kalajzic
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D009478:Neuropeptide Y / Q000235:genetics* / Q000378:metabolism; D010006:Osteoblasts / Q000378:metabolism*; D010011:Osteocytes / Q000378:metabolism*; D010012:Osteogenesis / Q000502:physiology*
chemical_list: D009478:Neuropeptide Y