title: Purification, physicochemical characterisation and anticancer activity of a polysaccharide from Cyclocarya paliurus leaves.
abstract: A Cyclocarya paliurus (Batal.) Iljinskaja polysaccharide (CPP) was isolated and purified by hot water extraction, ethanol precipitation, deproteinisation and anion-exchange chromatography. Its physicochemical properties were characterised by gel permeation chromatography (GPC), gas chromatography-mass spectrometry (GC-MS), thermal gravimetric analysis (TGA), Fourier transform infrared spectrometry (FTIR), UV-visible spectrophotometry, dynamic light scattering (DLS) and viscometry analysis. The anticancer effect of CPP in human gastric cancer HeLa cells was also evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results showed that the molecular weight of CPP was 900 kDa, and it contained 64.8% total sugar, 23.5% uronic acid, 9.26% protein, and six kinds of monosaccharides, including glucose, rhamnose, arabinose, xylose, mannose and galactose, with molar percentages of 32.7%, 9.33%, 30.6%, 3.48%, 10.4%, and 13.5%, respectively. Furthermore, the results showed that CPP exhibited a strong inhibition effect on the growth of human gastric cancer HeLa cells.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Xie
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Ming-Yue
authors.initials: MY
authors.lastname: Shen
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Nie
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Chang
authors.initials: C
authors.lastname: Li
authors.forename: De-Ming
authors.initials: DM
authors.lastname: Gong
authors.forename: Ming-Yong
authors.initials: MY
authors.lastname: Xie
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D029525:Juglandaceae / Q000737:chemistry*; D008970:Molecular Weight; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D014783:Viscosity
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D011134:Polysaccharides