title: Antimutagenic evaluation of vitamins B1, B6 and B12 in vitro and in vivo, with the Ames test.
abstract: The aim of this work is to evaluate vitamins B antimutagenic effect against alkylatings methyl-N-nitro-N-nitrosoguanidine (MNNG), ethyl-N-nitro-N'- nitrosoguanidine (ENNG), frameshift mutagens 2-aminoanthracene (2AA) and 2-acetyl-amino-fluorene (2AF) and ROS-generating antibiotics norfloxacin (NOR) and nalidixic acid (NLX), using the in vitro Ames test. In vivo antimutagenesis studies were performed against urinary mutagens induced by NOR (70 mg/kg) or NLX (100 mg/kg) in CD1 mice. Vitamin B1 was antimutagenic against alkylatings MNNG (P<0.05) or ENNG (P<0.001). In fact as per the results observed during the current study, none of the vitamins reduced mutagenesis caused by frameshift mutagens. All of them reduced mutagenesis of NOR or NLX (P<0.001). In vivo studies showed that vitamins B1 and B6 (10 or 100 mg/kg) reduced urinary mutagens from NOR (P<0.001) or NLX (P<0.02) either free or β-glucoronidase-conjugates. None of the studied samples were toxic for the employed antimutagenic system. Vitamin B12 (4 mg/kg) reduced urinary mutagens of NOR or NLX (P<0.02). Vitamins B inhibited DNA mutations induced by ROS generated by NLX or NOR, both in vitro and in vivo. Vitamin B1is antimutagenic against mutations induced by the alkylating MNNG or ENNG. Based on the observations, employment of vitamins B in vivo can be a promising alternative to reduce genotoxic risk exposure to ROS.
authors.affiliation: Microbiology Research Laboratory, Hospital Juárez de Mexico, Avenida Instituto Politécnico Nacional No. 5160, Colonia Magdalena de las Salinas, Mexico, DF 07760, Mexico. arriaga_alba@yahoo.com
authors.forename: Myriam
authors.initials: M
authors.lastname: Arriaga-Alba
authors.forename: Nancy Janett
authors.initials: NJ
authors.lastname: Ruiz-Pérez
authors.forename: Jaime
authors.initials: J
authors.lastname: Sánchez-Navarrete
authors.forename: Beatriz López
authors.initials: BL
authors.lastname: de Angel
authors.forename: Jorge
authors.initials: J
authors.lastname: Flores-Lozada
authors.forename: José Luis
authors.initials: JL
authors.lastname: Blasco
mesh_terms: D015073:2-Acetylaminofluorene / Q000032:analysis / Q000633:toxicity; D000818:Animals; D000873:Anthracenes / Q000032:analysis / Q000633:toxicity; D016587:Antimutagenic Agents / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects; D008769:Methylnitronitrosoguanidine / Q000031:analogs & derivatives / Q000032:analysis / Q000633:toxicity; D051379:Mice; D008815:Mice, Inbred Strains; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens; D009154:Mutation / Q000187:drug effects; D009643:Norfloxacin / Q000633:toxicity / Q000652:urine; D012486:Salmonella typhimurium / Q000187:drug effects; D013831:Thiamine / Q000494:pharmacology*; D014805:Vitamin B 12 / Q000494:pharmacology*; D025101:Vitamin B 6 / Q000494:pharmacology*
chemical_list: D000873:Anthracenes; D016587:Antimutagenic Agents; D009153:Mutagens; D008769:Methylnitronitrosoguanidine; C013525:ENNG; D025101:Vitamin B 6; C006593:2-anthramine; D015073:2-Acetylaminofluorene; D009643:Norfloxacin; D014805:Vitamin B 12; D013831:Thiamine