title: [Emergence of linezolid-resistant Enterococcus faecalis strains from two inpatients in a pediatric ward].
abstract: We report herein on the isolation of three linezolid-resistant Enterococcus faecalis strains in 2011 from two pediatric inpatients at Kitasato University Hospital, Japan. Three linezolid resistant strains were isolated from two patients who shared the same room of a pediatric inpatient ward. Two linezolid resistant strains were isolated from patient A who had been treated with a total of 17,600mg of linezolid during 60 days of hospitalization (strains 1 and 2). The linezolid resistant E. faecalis persisted through the time that the patient had been discharged from the hospital. Another linezolid resistant strain was isolated from patient B who had no history of linezolid administration. The resistant strain in patient B phased out spontaneously. The minimum inhibitory concentration of linezolid in these strains ranged from 8.0 to 16.0 microg/mL. PCR amplification of the chromosomal gene encoding domain V of the 23S rRNA and subsequent nucleotide sequencing revealed that all the strains had at least one G2576T mutation. The pulse-field-gel electrophoretograms of the DNA treated with the SmaI restriction enzyme showed an identical profile suggesting that they were derived from a single resistant strain. These results suggested that the resistant strain occurred in patient A and was transmitted to patient B within the inpatient ward.
authors.affiliation: Department of Medical Laboratory, Kitasato University Hospital.
authors.forename: Shin
authors.initials: S
authors.lastname: Nihonyanagi
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Adachi
authors.forename: Tomoyo
authors.initials: T
authors.lastname: Onuki
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Nakazaki
authors.forename: Yasuyosi
authors.initials: Y
authors.lastname: Hirata
authors.forename: Kuniko
authors.initials: K
authors.lastname: Fujiki
authors.forename: Yoko
authors.initials: Y
authors.lastname: Takayama
authors.forename: Yuhsaku
authors.initials: Y
authors.lastname: Kanoh
authors.forename: Yuki
authors.initials: Y
authors.lastname: Bandoh
authors.forename: Yurika
authors.initials: Y
authors.lastname: Dantsuji
authors.forename: Hideaki
authors.initials: H
authors.lastname: Hanaki
authors.forename: Keisuke
authors.initials: K
authors.lastname: Sunakawa
mesh_terms: D000081:Acetamides / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002648:Child; D002675:Child, Preschool; D024881:Drug Resistance, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D013293:Enterococcus faecalis / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D000069349:Linezolid; D008297:Male; D023303:Oxazolidinones / Q000494:pharmacology*; D016133:Polymerase Chain Reaction
chemical_list: D000081:Acetamides; D000890:Anti-Infective Agents; D023303:Oxazolidinones; D000069349:Linezolid