title: A dominant gene for male sterility in Salvia miltiorrhiza Bunge.
abstract: A natural male sterile mutant of Salvia miltiorrhiza (Labiatae, Sh-B) was found during field survey in 2002. Our objective was to analyze its genetic mechanism for producing F1 hybrid seeds and to develop a molecular marker linked to male sterile gene for selection of a hybrid parent line. The segregation ratios of male sterile plants to fertile plants in the progenies of both testcross and backcross were 1:1 in continuous experiments conducted in 2006-2009. The male sterile Sh-B was heterozygous (Msms). The male sterile plants could capture most pollen (2 granule/cm(2)·24 h) with row ratio (female:male 2:1) within 45-cm distance and harvest the largest amount of 6495 g hybrid seeds per hectare. We also developed DNA markers linked to the male sterile gene in a segregating population using bulked segregant analysis (BSA) and amplified fragment length polymorphism (AFLP) techniques. The segregating population was subjected to BSA-AFLP with 128 primer combinations. One out of fourteen AFLP markers (E11/M4208) was identified as tightly linked to the dominant male sterile gene with a recombination frequency of 6.85% and at a distance of 6.89 cM. This marker could be converted to PCR-based assay for large-scale selection of fertile plants in MAS (marker-assisted selection) at the seedling stage. Blastn analysis indicated that the male sterile gene sequence showed higher identity with nucleotides in Arabidopsis chromosome 1-5, and was more likely to encode S-adenosylmethionine-dependent methyltransferase, in which DNA methylation regulated the development of plant gametogenesis.
authors.affiliation: Shaanxi Research Center of TCM Fingerprinting and NP Library, College of Life Sciences, Northwest A&F University, Yangling, PR China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Shu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Mu
authors.forename: Zongsuo
authors.initials: Z
authors.lastname: Liang
authors.forename: Hongbo
authors.initials: H
authors.lastname: Guo
mesh_terms: D017343:Genes, Plant / Q000235:genetics / Q000502:physiology*; D051479:Plant Infertility / Q000235:genetics / Q000502:physiology*; D027561:Salvia miltiorrhiza / Q000235:genetics* / Q000502:physiology*