title: Effect of Auxin on Rooting and Endogenous Cytokinin Levels in Leaf Cuttings of Phaseolus vulgaris L.
abstract: Treatment of the petioles of leaf explants of Phaseolus vulgaris L. with indole-butyric acid stimulated root initiation and root development on the petioles. Best results were obtained with a concentration of 20 mg 1(-1) of the auxin. Immediately after the auxin treatment the levels of endogenous cytokinins in the treated explants was higher than in the untreated material. Most of this detected cytokinin was found in the leaf lamina. Auxin treatment apparently resulted not only in increased levels of non-polar cytokinins in the explants but apparently also brought about a redistribution of these hormones. This resulted in low levels of these hormones in the treated petioles, a condition which is well documented to be favourable for the rooting process.
authors.affiliation: Plant Development Research Unit, Department of Botany, University of Natal, Pietermaritzburg 3200, South Africa.
authors.forename: S S
authors.initials: SS
authors.lastname: Bridglall
authors.forename: J
authors.initials: J
authors.lastname: Van Staden