title: MAGE-C1/CT7 and MAGE-C2/CT10 are frequently expressed in multiple myeloma and can be explored in combined immunotherapy for this malignancy.
abstract: The exact function of MAGE-C1/CT7 and MAGE-C2/CT10 is not yet understood in multiple myeloma (MM). However, the homologs MAGE-C1/CT7 and MAGE-C2/CT10 genes encode highly immunogeneic cancer/testis antigens (CTAs) and can be potential targets for T cell-based immunotherapy. MAGE-C1/CT7 and MAGE-C2/CT10 mRNA expression were investigated in MM patients, solitary plasmacytomas, monoclonal gammopathies of undetermined significance (MGUS) and bone marrow (BM) aspirates from healthy donors by RT-PCR. MAGE-C1/CT7 and MAGE-C1/CT10 were expressed in 67 and 59 % of the 46 MM analyzed patients. At least one of the genes was expressed in 76 % of MM cases. Solitary plasmacytoma also showed MAGE-C1/CT7 and MAGE-C2/CT10 expression. MAGE-C1/CT7 and MAGE-C2/CT10 were not expressed in normal BM samples, showing restricted expression of these CTA genes in MM, solitary plasmacytoma and MGUS. In the present study, we found high expression of the homologs MAGE-C1/CT7 and MAGE-C2/CT10 in monoclonal gammopathies and speculate whether these genes might represent a valuable therapeutic option for myeloma, in particular for combined immunotherapy.
authors.affiliation: Disciplina de Hematologia e Hemoterapia, Universidade Federal de São Paulo (UNIFESP/EPM), Rua Botucatu, 740, 3o andar, Hematologia, Vila Clementino, CEP 04023-900, São Paulo, Brazil. fabrocarvalho@gmail.com
authors.forename: Fabricio
authors.initials: F
authors.lastname: de Carvalho
authors.forename: Veruska L F
authors.initials: VL
authors.lastname: Alves
authors.forename: Walter M T
authors.initials: WM
authors.lastname: Braga
authors.forename: Celso V
authors.initials: CV
authors.lastname: Xavier
authors.forename: Gisele W B
authors.initials: GW
authors.lastname: Colleoni
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000032:analysis / Q000096:biosynthesis*; D015415:Biomarkers / Q000032:analysis; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007167:Immunotherapy; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000276:immunology / Q000378:metabolism* / Q000628:therapy*; D009363:Neoplasm Proteins / Q000032:analysis / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D059467:Transcriptome
chemical_list: D000951:Antigens, Neoplasm; D015415:Biomarkers; C110905:MAGEC1 protein, human; C405429:MAGEC2 protein, human; D009363:Neoplasm Proteins