title: Genomic analysis of a Canadian equine rhinitis A virus reveals low diversity among field isolates.
abstract: Equine rhinitis A virus (ERAV) is an ubiquitous virus, routinely identified in equine respiratory infections; however, its role in disease and genetic features are not well defined due to a lack of genomic characterization of the recovered isolates. Therefore, we sequenced the full-length genome of a Canadian ERAV (ERAV/ON/05) and compared it with other ERAV sequences currently available in GenBank. The ERAV/ON/05 genome is 7,839 nucleotides (nts) in length with a variable 5'UTR and a more conserved 3'UTR. When ERAV/ON/05 was compared to other reported ERAV isolates, an insertion of 13 nt in the 5'UTR was identified. Further phylogenetic analysis demonstrated that ERAV/ON/05 is closely related to the ERAV/PERV isolate, which was isolated in 1962 in the United Kingdom. The polyprotein of ERAV/ON/05 had a 96 % nucleotide and amino acid sequence identity to reported ERAVs, and it appears that, despite the high error rate of RNA-dependent RNA polymerase, this isolate has retained high sequence identity to the strain first described by Plummer in 1962.
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, N1G 2W1, Canada. adiaz@uoguelph.ca
authors.forename: Andrés
authors.initials: A
authors.lastname: Diaz-Méndez
authors.forename: Laurent
authors.initials: L
authors.lastname: Viel
authors.forename: Patricia
authors.initials: P
authors.lastname: Shewen
authors.forename: Eva
authors.initials: E
authors.lastname: Nagy
mesh_terms: D000818:Animals; D005537:Aphthovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D014644:Genetic Variation; D023281:Genomics; D006734:Horse Diseases / Q000821:virology*; D006736:Horses; D008969:Molecular Sequence Data; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000662:veterinary* / Q000821:virology