title: Crystallization and preliminary X-ray diffraction analysis of mevalonate kinase from Methanosarcina mazei.
abstract: Mevalonate kinase (MVK), which plays an important role in catalysing the biosynthesis of isoprenoid compounds derived from the mevalonate pathway, transforms mevalonate to 5-phosphomevalonate using ATP as a cofactor. Mevalonate kinase from Methanosarcina mazei (MmMVK) was expressed in Escherichia coli, purified and crystallized for structural analysis. Diffraction-quality crystals of MmMVK were obtained by the vapour-diffusion method using 0.32 M MgCl2, 0.08 M bis-tris pH 5.5, 16%(w/v) PEG 3350. The crystals belonged to space group P2(1)2(1)2, with unit-cell parameters a=97.11, b=135.92, c=46.03 Å. Diffraction data were collected to 2.08 Å resolution.
authors.affiliation: Division of Applied Life Science (BK21 Program), Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Ningning
authors.initials: N
authors.lastname: Zhuang
authors.forename: Kyung Hye
authors.initials: KH
authors.lastname: Seo
authors.forename: Cong
authors.initials: C
authors.lastname: Chen
authors.forename: Jia
authors.initials: J
authors.lastname: Zhou
authors.forename: Seon Won
authors.initials: SW
authors.lastname: Kim
authors.forename: Kon Ho
authors.initials: KH
authors.lastname: Lee
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D002384:Catalysis; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D017020:Methanosarcina / Q000201:enzymology*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry* / Q000378:metabolism; D014325:Tromethamine / Q000031:analogs & derivatives; D014961:X-Ray Diffraction
chemical_list: D014325:Tromethamine; D000255:Adenosine Triphosphate; D017853:Phosphotransferases (Alcohol Group Acceptor); C021796:mevalonate kinase; C026272:Bistris