title: Use of an electrodialytic reactor for the simultaneous β-lactoglobulin enzymatic hydrolysis and fractionation of generated bioactive peptides.
abstract: The enzymatic hydrolysis of β-lactoglobulin and the fractionation of peptides were performed in one step in an electrodialysis cell with ultrafiltration membranes stacked. After 240 min of treatment, 15 anionic and 4 cationic peptides were detected in the anionic and cationic peptide recovery compartments. Amongst these 15 anionic peptides, 2 hypocholesterolemic, 3 antihypertensive and 1 antibacterial peptides were recovered and concentrated with migration rates ranging from 5.5% and 81.7%. Amongst the 4 cationic peptides, the peptide sequence ALPMHIR, identified as lactokinin and known to exert an important antihypertensive effect, was recovered with an estimated 66% migration rate. To our knowledge, it was the first attempt to perform hydrolysis under an electric field and to simultaneously separate anionic and cationic peptides produced.
authors.affiliation: Department of Food Science and Nutrition, Institute of Nutraceuticals and Functional Foods (INAF), Dairy Research Center (STELA), Université Laval, Québec, QC, Canada G1V 0A6.
authors.forename: Alain
authors.initials: A
authors.lastname: Doyen
authors.forename: Eric
authors.initials: E
authors.lastname: Husson
authors.forename: Laurent
authors.initials: L
authors.lastname: Bazinet
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003956:Dialysis / Q000295:instrumentation / Q000379:methods*; D006868:Hydrolysis; D007782:Lactoglobulins / Q000737:chemistry*; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification; D014357:Trypsin / Q000737:chemistry*; D014462:Ultrafiltration
chemical_list: D007782:Lactoglobulins; D010455:Peptides; D014357:Trypsin