title: Interpretation of protein quantitation using the Bradford assay: comparison with two calculation models.
abstract: The Bradford assay is a simple method for protein quantitation, but variation in the results between proteins is a matter of concern. In this study, we compared and normalized quantitative values from two models for protein quantitation, where the residues in the protein that bind to anionic Coomassie Brilliant Blue G-250 comprise either Arg and Lys (Method 1, M1) or Arg, Lys, and His (Method 2, M2). Use of the M2 model yielded much more consistent quantitation values compared with use of the M1 model, which exhibited marked overestimations against protein standards.
authors.affiliation: Center for Bioanalysis, Division of Metrology for Quality of Life, Korea Research Institute of Standards and Science, Department of Bioanalytical Science, University of Science and Technology, Daejeon 305-340, Republic of Korea.
authors.forename: Hyung-Keun
authors.initials: HK
authors.lastname: Ku
authors.forename: Hyuk-Min
authors.initials: HM
authors.lastname: Lim
authors.forename: Kyong-Hwa
authors.initials: KH
authors.lastname: Oh
authors.forename: Hyo-Jin
authors.initials: HJ
authors.lastname: Yang
authors.forename: Ji-Seon
authors.initials: JS
authors.lastname: Jeong
authors.forename: Sook-Kyung
authors.initials: SK
authors.lastname: Kim
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001120:Arginine / Q000737:chemistry; D002417:Cattle; D006639:Histidine / Q000737:chemistry; D006801:Humans; D061385:Insulins / Q000737:chemistry / Q000378:metabolism; D008239:Lysine / Q000737:chemistry; D008962:Models, Theoretical; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D012394:Rosaniline Dyes / Q000737:chemistry* / Q000378:metabolism; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism; D013053:Spectrophotometry
chemical_list: D016229:Amyloid beta-Peptides; D061385:Insulins; D010446:Peptide Fragments; D011506:Proteins; D012394:Rosaniline Dyes; D012709:Serum Albumin; C075222:amyloid beta-protein (1-42); D006639:Histidine; D001120:Arginine; D008239:Lysine; C004692:coomassie Brilliant Blue