title: Possible regulation of Oct60 transcription by a positive feedback loop in Xenopus oocytes.
abstract: The POU family subclass V (POU-V) proteins have important roles in maintaining cells in an undifferentiated state. In Xenopus, expression of the POU-V protein Oct60 was detected in oocytes and was found to decrease in blastula- to gastrula-stage embryos. In addition, Oct60 overexpression inhibits some signals in early embryogenesis, including Activin/Nodal, BMP, and Wnt signalling. In this report, we analysed mechanisms of Oct60 promoter activation and discovered that Oct60 transcription was activated ectopically in somatic nuclei by oocyte extract treatment. Promoter assays demonstrated that Oct60 transcription was activated in oocytes specifically and that this activation was dependent on an Octamer-Sox binding motif. ChIP assays showed that the Oct60 protein binds the motif. These results suggest that Oct60 transcription is regulated by a positive-feedback loop in Xenopus oocytes.
authors.affiliation: Department of Life Science, School of Science, Rikkyo University, 3-34-1 Nishi-ikebukuro, Toshima, Tokyo 178-8501, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Morichika
authors.affiliation: Department of Life Science, School of Science, Rikkyo University, 3-34-1 Nishi-ikebukuro, Toshima, Tokyo 178-8501, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Sugimoto
authors.affiliation: Department of Bioscience, School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, Sanda, Hyogo, 669-1337, Japan.
authors.forename: Katsuki
authors.initials: K
authors.lastname: Yasuda
authors.affiliation: Department of Life Science, School of Science, Rikkyo University, 3-34-1 Nishi-ikebukuro, Toshima, Tokyo 178-8501, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Kinoshita
mesh_terms: D000818:Animals; D001483:Base Sequence; D036703:Blastula / Q000166:cytology / Q000502:physiology; D047369:Chromatin Immunoprecipitation; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000502:physiology*; D025461:Feedback, Physiological; D005260:Female; D005775:Gastrula / Q000166:cytology / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D008156:Luciferases; D008969:Molecular Sequence Data; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D050809:POU Domain Factors; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D015398:Signal Transduction; D014158:Transcription, Genetic / Q000235:genetics*; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics / Q000378:metabolism*
chemical_list: D050809:POU Domain Factors; D012333:RNA, Messenger; D029867:Xenopus Proteins; D008156:Luciferases