title: A single-blood-sample method using inulin for estimating feline glomerular filtration rate.
abstract: BACKGROUND
  Application of a multisample method using inulin to estimate glomerular filtration rate (GFR) in cats is cumbersome.
  
  
  OBJECTIVE
  To establish a simplified procedure to estimate GFR in cats, a single-blood-sample method using inulin was compared with a conventional 3-sample method.
  
  
  METHODS
  Nine cats including 6 clinically healthy cats and 3 cats with spontaneous chronic kidney disease.
  
  
  METHODS
  Retrospective study. Inulin was administered as an intravenous bolus at 50 mg/kg to cats, and blood was collected at 60, 90, and 120 minutes later for the 3-sample method. Serum inulin concentrations were colorimetrically determined by an autoanalyzer method. The GFR in the single-blood-sample method was calculated from the dose injected, serum concentration, sampling time, and estimated volume of distribution on the basis of the data of the 3-sample method.
  
  
  RESULTS
  An excellent correlation was observed (r = 0.99, P = .0001) between GFR values estimated by the single-blood-sample and 3-sample methods.
  
  
  CONCLUSIONS
  The single-blood-sample method using inulin provides a practicable and ethical alternative for estimating glomerular filtration rate in cats.
authors.affiliation: Department of Veterinary Clinical Medicine, Iwate University, Morioka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Katayama
authors.forename: J
authors.initials: J
authors.lastname: Saito
authors.forename: R
authors.initials: R
authors.lastname: Katayama
authors.forename: N
authors.initials: N
authors.lastname: Yamagishi
authors.forename: I
authors.initials: I
authors.lastname: Murayama
authors.forename: A
authors.initials: A
authors.lastname: Miyano
authors.forename: K
authors.initials: K
authors.lastname: Furuhama
mesh_terms: D000818:Animals; D001800:Blood Specimen Collection / Q000662:veterinary; D002415:Cats / Q000502:physiology*; D003124:Colorimetry / Q000662:veterinary; D005919:Glomerular Filtration Rate / Q000502:physiology / Q000662:veterinary*; D007444:Inulin / Q000097:blood / Q000493:pharmacokinetics; D007668:Kidney / Q000502:physiology*; D051436:Renal Insufficiency, Chronic / Q000097:blood / Q000503:physiopathology / Q000662:veterinary*; D012189:Retrospective Studies
chemical_list: D007444:Inulin