title: Expression of ESE-3 isoforms in immunogenic and tolerogenic human monocyte-derived dendritic cells.
abstract: Dendritic cells (DC) are the only hematopoietic cells expressing the epithelial specific Ets transcription factor ESE-3. Here we analyzed presence and quantity of isoforms ESE-3a, ESE-3b and ESE-3j in various immunogenic and tolerogenic human monocyte-derived DC (moDC) and blood DC populations using quantitative real time PCR and immunoblot analyses. ESE-3a and ESE-3b were detectable in all moDC populations with ESE-3b being the main transcript. ESE-3b expression was upregulated in immunogenic moDC and downregulated in tolerogenic moDC compared to immature moDC. ESE-3a had similar transcript levels in immature and immunogenic moDC and had very low levels in tolerogenic moDC. In blood DC populations only splice variant ESE-3b was detectable. ESE-3j was not detectable in any of the DC populations. These findings suggest that ESE-3b is the functionally most important ESE-3 isoform in DC.
authors.affiliation: Broegelmann Research Laboratory, The Gade Institute, University of Bergen, Bergen, Norway.
authors.forename: Florian
authors.initials: F
authors.lastname: Sprater
authors.forename: Arnt-Ove
authors.initials: AO
authors.lastname: Hovden
authors.forename: Silke
authors.initials: S
authors.lastname: Appel
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology*; D005091:Exons; D005434:Flow Cytometry / Q000379:methods; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D007107:Immune System; D015151:Immunoblotting / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods; D007167:Immunotherapy / Q000379:methods; D008954:Models, Biological; D008957:Models, Genetic; D009000:Monocytes / Q000166:cytology*; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D020033:Protein Isoforms; D050783:Proto-Oncogene Proteins c-ets / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*
chemical_list: C403306:EHF protein, human; D020033:Protein Isoforms; D050783:Proto-Oncogene Proteins c-ets; D014157:Transcription Factors