title: Application of horseradish peroxidase modified nanostructured Au thin films for the amperometric detection of 4-chlorophenol.
abstract: A facile electrodeposition method was used to fabricate nanostructured Au thin films. Construction of the amperometric biosensors for 4-chlorophenol (4-CP) is based on the immobilization of horseradish peroxidase (HRP) on the Au thin films via a simple method. The as-prepared nano-Au films provide a favorable microenvironment for the immobilization of HRP and the retention of its activity. With H(2)O(2) as oxidizing co-substrate, the HRP modified electrode displays high catalytic activity toward 4-CP. Besides, it has been demonstrated that the immobilization of HRP has a significant positive effect on the anti-fouling performance of the electrode material. Furthermore, the enzyme modified electrode was used as a bioelectrochemical sensor of 4-CP, exhibiting linear relationship in two different concentration ranges: from 2.5 to 40 μM and from 62.5 to 117.5 μM, with a detection limit of 0.39 μM (S/N=3) at an applied potential of -0.55 V. The fast current response and good stability were obtained on the HRP modified electrode.
authors.affiliation: Key Laboratory for Colloid and Interface Chemistry of State Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China.
authors.forename: Cuicui
authors.initials: C
authors.lastname: Qiu
authors.forename: Ting
authors.initials: T
authors.lastname: Chen
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Houyi
authors.initials: H
authors.lastname: Ma
mesh_terms: D057219:Biofouling / Q000517:prevention & control; D002244:Carbon / Q000737:chemistry; D002384:Catalysis / Q000187:drug effects; D002733:Chlorophenols / Q000032:analysis*; D004560:Electricity; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism; D005898:Glass / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction / Q000187:drug effects; D015203:Reproducibility of Results; D014867:Water / Q000737:chemistry
chemical_list: D002733:Chlorophenols; D004800:Enzymes, Immobilized; D014867:Water; C029107:4-chlorophenol; D002244:Carbon; D006046:Gold; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase