title: Theileria, Babesia, and Anaplasma detected by PCR in ruminant herds at Bié Province, Angola.
abstract: Distribution of Anaplasma spp., Babesia spp., Theileria spp., and Ehrlichia ruminantium, was for the first time studied in Bié Province, central Angola. We examined 76 blood samples of cattle originated from seven farms, and 13 blood samples of goats from two farms employing molecular genetic tools (PCR). Most prevalent was A. ovis-infection in goats (100%) and A. marginale-infection in cattle (38% of examined animals, and six out of seven farms). B. bigemina-infection was detected in only one specimen at Andulo, whereas B. bovis was not detected in Bié. We did not detected T. parva, the causative agent of serious diseases in cattle; nevertheless, infection by T. velifera was quite frequent (14% of examined animals, and five out of seven farms). Causative agent of heartwater disease - E. ruminantium, was not detected. Taking into account short-term perspective of PCR methods in monitoring of epidemiological status in herds, the number of infected animals and distribution of detected pathogens should not be ignored.
authors.affiliation: Department of Biology and Wildlife Diseases, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Kubelová
authors.forename: J
authors.initials: J
authors.lastname: Mazancová
authors.forename: P
authors.initials: P
authors.lastname: Siroký
mesh_terms: D000709:Anaplasma / Q000235:genetics / Q000302:isolation & purification; D000712:Anaplasmosis / Q000453:epidemiology* / Q000382:microbiology; D000810:Angola / Q000453:epidemiology; D000818:Animals; D001403:Babesia / Q000235:genetics / Q000302:isolation & purification; D001404:Babesiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000382:microbiology / Q000469:parasitology; D004269:DNA, Bacterial / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification; D015511:Goat Diseases / Q000453:epidemiology* / Q000382:microbiology / Q000469:parasitology; D006041:Goats; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016794:Theileria / Q000235:genetics / Q000302:isolation & purification; D013801:Theileriasis / Q000453:epidemiology* / Q000469:parasitology; D017282:Tick-Borne Diseases / Q000453:epidemiology / Q000382:microbiology / Q000469:parasitology / Q000662:veterinary
chemical_list: D004269:DNA, Bacterial; D016054:DNA, Protozoan