title: Dansyl-labeled cholic acid as a tool to build speciation diagrams for the aggregation of bile acids.
abstract: Bile acids (BAs) are a family of natural steroids biosynthesized from cholesterol in the liver that tend to form aggregates in solution. A fluorescent derivative of cholic acid, namely 3α-Dns-ChA, was employed as a reporter to establish the speciation diagrams of the most abundant BAs that can be found mainly in three microenvironments, solution, and primary and secondary aggregates. The developed methodology is based on the analysis of the combined steady-state and time-resolved experiments performed on 3α-Dns-ChA whose emission behavior was found to be strongly medium dependent. In particular, speciation diagrams of sodium glycocholate (NaGCh), sodium taurocholate (NaTCh), sodium chenodeoxycholate (NaCDCh), sodium glycochenodeoxycholate (NaGCDCh), sodium deoxycholate (NaDCh), and sodium ursodeoxycholate (NaUDCh) were successfully built up.
authors.affiliation: Instituto Universitario Mixto de Tecnología Química (UPV-CSIC), Universitat Politècnica de València, 46022 Valencia, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Gomez-Mendoza
authors.forename: M Luisa
authors.initials: ML
authors.lastname: Marin
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Miranda
mesh_terms: D001647:Bile Acids and Salts / Q000737:chemistry*; D019826:Cholic Acid / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry*; D011789:Quantum Theory; D013050:Spectrometry, Fluorescence
chemical_list: C496906:1-myristoyl-2-(12-((5-dimethylamino-1-naphthalenesulfonyl)amino)dodecanoyl)-sn-glycero-3-phosphocholine; D001647:Bile Acids and Salts; D010713:Phosphatidylcholines; D019826:Cholic Acid