title: Isolation, identification and antimicrobial activities of two secondary metabolites of Talaromyces verruculosus.
abstract: From the ethyl acetate extract of the culture broth of Talaromyces verruculosus, a rhizosphere fungus of Stellera chamaejasme L., (-)-8-hydroxy-3-(4-hydroxypentyl)-3,4-dihydroisocoumarin (1) and (E)-3-(2,5-dioxo-3-(propan-2-ylidene)pyrrolidin-1-yl)acrylic acid (2) were isolated and evaluated for their antimicrobial activities. Their structures were elucidated by UV, IR, MS, 1H-NMR, 13C-NMR and 2D NMR spectra. Compound 1 exhibited the significant activities in vitro against two strains of bacteria and four strains of fungi. Compound 2 gave slight activities on the fungi at 100 µg mL(-1), but no activities on the bacteria. Compound 1 should be considered as a new lead or model compound to develop new isocoumarin antimicrobial agents.
authors.affiliation: College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Miao
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.forename: Dong-Dong
authors.initials: DD
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Bao-Fu
authors.initials: BF
authors.lastname: Qin
authors.forename: Xin-Juan
authors.initials: XJ
authors.lastname: Yang
authors.forename: Le
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000179:Acrylates / Q000737:chemistry*; D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D001419:Bacteria / Q000187:drug effects; D003374:Coumarins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D005658:Fungi / Q000187:drug effects; D049934:Isocoumarins; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D011759:Pyrrolidines / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D032901:Talaromyces / Q000737:chemistry / Q000378:metabolism
chemical_list: C581276:(-)-8-hydroxy-3-(4-hydroxypentyl)-3,4-dihydroisocoumarin; C581277:(E)-3-(2,5-dioxo-3-(propan-2-ylidene)pyrrolidin-1-yl)acrylic acid; D000179:Acrylates; D000890:Anti-Infective Agents; D003374:Coumarins; D049934:Isocoumarins; D011759:Pyrrolidines; C030123:coumarin; C032519:pyrrolidine