title: [Genetic characterization of haemagglutinin of novel H1N1 influenza viruses in Suzhou City].
abstract: OBJECTIVE
  To investigate the genetic variation and molecular characteristics of HA gene of influenza A (H1N1) virus isolated in Suzhou during from2009 to 2011.
  
  
  METHODS
  Viral RNA of 5 Suzhou isolates was isolated and their HA gene were amplified and sequenced by the primers and protocol recommended by WHO, and the sequences together with other sequences downloaded from GenBank were analyzed by several bioinformatics software.
  
  
  RESULTS
  Compared with vaccine strain, the average homogeneity of nucleotide and amino acids of 5 Suzhou isolates were between 98.8-99.4% and 98.8-99.4% respectively. All of the 5 strains have 1 amino acids replacement in Sb region and 2 strains have 2 amino acids replacement in Ca region. Strains from and outside Suzhou both showed a trend of clustering by collection year.
  
  
  CONCLUSIONS
  The Suzhou strains are still in stable condition although 1-2 amino acids replacement had happened in antigenic sites.
authors.affiliation: Suzhou Center for Disease Control and Prevention, Suzhou, Jiangsu 215004, China.
authors.forename: Wei-Ning
authors.initials: WN
authors.lastname: Han
authors.forename: Yu
authors.initials: Y
authors.lastname: Xia
authors.forename: Xue-Rong
authors.initials: XR
authors.lastname: Ya
authors.forename: Qiang
authors.initials: Q
authors.lastname: Shen
authors.forename: Hao
authors.initials: H
authors.lastname: Pan
mesh_terms: D000595:Amino Acid Sequence; D002681:China; D019143:Evolution, Molecular; D014644:Genetic Variation; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D007251:Influenza, Human / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus