title: Animal model for training in sentinel lymph node biopsy of the stomach through combined methods.
abstract: OBJECTIVE
  Create and validate a proposed animal model for training in sentinel lymph node biopsy of the stomach.
  
  
  METHODS
  In thirty-two rabbits, through a laparotomy, they received a subserosal injection of 0.1 ml of phytate labeled with technetium-99m (0.2 mCi) in the anterior wall of the gastric corpus, followed by 0.2 ml of Blue Patent V(®) 2.5%, through the same puncture site. Suspicious lymph nodes were searched in vivo at five, ten and 20 minutes, both visually (Blue Patent stained lymph nodes) and with a manual gamma radiation detector (to detect suspected radioactive lymph nodes). After 20 minutes, was performed resection of these for further evaluation of radioactivity (ex vivo) and histological study.
  
  
  RESULTS
  Lymph nodes were identified in 30 rabbits (Average of 2.2 lymph nodes per animal). Of the 90 suspected lymph nodes that occurred in the study, 70 cases (77.8%) were histologically confirmed for lymphoid tissue. Of these, the majority were located in the periesophageal region of the gastric fundus. The sample presented a mortality rate of 6.25% and nine complications related to the method, which interfered in the identification of the lymph nodes.
  
  
  CONCLUSIONS
  The animal model for sentinel node biopsy in rabbit stomachs proved to be feasible, with low complexity and reproduced the difficulties encountered for gastric lymph node biopsy in humans, being adequate for surgical training.
authors.affiliation: UNICAMP, Campinas, SP, Brazil. joserobertoalves1980@gmail.com
authors.forename: José Roberto
authors.initials: JR
authors.lastname: Alves
authors.forename: Luiz Roberto
authors.initials: LR
authors.lastname: Lopes
authors.forename: Luciana Rodrigues de
authors.initials: LR
authors.lastname: Meirelles
authors.forename: Celso Darío
authors.initials: CD
authors.lastname: Ramos
authors.forename: Tânia
authors.initials: T
authors.lastname: Sasasaki
authors.forename: Nelson Adami
authors.initials: NA
authors.lastname: Andreollo
mesh_terms: D000818:Animals; D001707:Biopsy, Needle; D004195:Disease Models, Animal; D008198:Lymph Nodes / Q000000981:diagnostic imaging / Q000473:pathology*; D008297:Male; D011817:Rabbits; D011877:Radionuclide Imaging; D021701:Sentinel Lymph Node Biopsy / Q000193:education / Q000379:methods*; D013270:Stomach / Q000473:pathology; D013667:Technetium
chemical_list: D013667:Technetium