title: A potential practical approach to reduce Ara h 6 allergenicity by gamma irradiation.
abstract: Peanut allergen Ara h 6 was isolated and irradiated at 1, 3, 5, or 10 kGy, and a whole peanut protein extract (WPPE) was also treated by irradiation. Alteration in structure of Ara h 6 was characterised by circular dichroism (CD) spectroscopy, ultraviolet (UV) absorption spectroscopy, fluorescence spectroscopy and SDS-PAGE, and antigenicity was evaluated by immunoblotting and indirect ELISA with anti-Ara h 6 polyclonal antibody. Irradiation induced significant changes in the secondary and tertiary structures of Ara h 6, and the antigenicity of both purified Ara h 6 and WPPE were reduced upon increasing the irradiation doses. Moreover, a good correlation between the loss in α-helix and IgG binding to Ara h 6 was observed. This indicated that irradiation might be an efficient approach to reduce or eliminate peanut allergenicity.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Luo
authors.forename: Chunqiu
authors.initials: C
authors.lastname: Hu
authors.forename: Jinyan
authors.initials: J
authors.lastname: Gao
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Wu
authors.forename: Anshu
authors.initials: A
authors.lastname: Yang
authors.forename: Hongbing
authors.initials: H
authors.lastname: Chen
mesh_terms: D055516:2S Albumins, Plant / Q000737:chemistry / Q000276:immunology*; D052179:Antigens, Plant / Q000737:chemistry / Q000276:immunology*; D010367:Arachis / Q000737:chemistry / Q000276:immunology* / Q000528:radiation effects*; D002942:Circular Dichroism; D005514:Food Irradiation / Q000379:methods*; D005720:Gamma Rays; D007073:Immunoglobulin E / Q000276:immunology
chemical_list: D055516:2S Albumins, Plant; D052179:Antigens, Plant; C492737:Ara h 6 allergen, Arachis hypogaea; D007073:Immunoglobulin E