title: Zwitterionic polymer-modified silicon microring resonators for label-free biosensing in undiluted human plasma.
abstract: A widely acknowledged goal in personalized medicine is to radically reduce the costs of highly parallelized, small fluid volume, point-of-care and home-based diagnostics. Recently, there has been a surge of interest in using complementary metal-oxide-semiconductor (CMOS)-compatible silicon photonic circuits for biosensing, with the promise of producing chip-scale integrated devices containing thousands of orthogonal sensors, at minimal cost on a per-chip basis. A central challenge in biosensor translation is to engineer devices that are both sensitive and specific to a target analyte within unprocessed biological fluids. Despite advances in the sensitivity of silicon photonic biosensors, poor biological specificity at the sensor surface remains a significant factor limiting assay performance in complex media (i.e. whole blood, plasma, serum) due to the non-specific adsorption of proteins and other biomolecules. Here, we chemically modify the surface of silicon microring resonator biosensors for the label-free detection of an analyte in undiluted human plasma. This work highlights the first application of a non-fouling zwitterionic surface coating to enable silicon photonic-based label-free detection of a protein analyte at clinically relevant sensitivities in undiluted human plasma.
authors.affiliation: Department of Bioengineering, University of Washington, Box 355061, Seattle, WA 98195, USA. jtk8@uw.edu
authors.forename: James T
authors.initials: JT
authors.lastname: Kirk
authors.forename: Norman D
authors.initials: ND
authors.lastname: Brault
authors.forename: Tom
authors.initials: T
authors.lastname: Baehr-Jones
authors.forename: Michael
authors.initials: M
authors.lastname: Hochberg
authors.forename: Shaoyi
authors.initials: S
authors.lastname: Jiang
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Ratner
mesh_terms: D026921:Amino Acid Transport Systems, Neutral / Q000737:chemistry*; D015374:Biosensing Techniques; D006801:Humans; D011108:Polymers / Q000737:chemistry; D012666:Semiconductors; D044967:Serum / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry*
chemical_list: D026921:Amino Acid Transport Systems, Neutral; D011108:Polymers; D012825:Silicon