title: Neuroprotective effects of methyl-3-O-methyl gallate against sodium fluoride-induced oxidative stress in the brain of rats.
abstract: Methyl-3-O-methyl gallate (M3OMG) is a rare natural product that showed promising in vitro antioxidant activities. In this study, the protective role of synthetic M3OMG against sodium fluoride (NaF)-induced oxidative stress in rat brain was evaluated. Animals were treated with either M3OMG (10 and 20 mg/kg i.p.), vitamin C (10 mg/kg i.p.) as the standard antioxidant or the vehicle (5 % dimethyl sulfoxide; 1 ml/kg) for 1 week. Oxidative stress was induced in the brain by adding 600 ppm NaF in the drinking water for 7 days. At the end of the treatment period, the levels of thiobarbituric acid reactive substances (TBARS), reduced glutathione and the activities of antioxidant enzymes (superoxide dismutase and catalase) were evaluated in brain homogenates. M3OMG treatment mitigated the NaF-induced oxidative stress through normalization of the level of TBARS, reduced levels of glutathione and by the restoration of the diminished antioxidant enzyme activities. In conclusion, M3OMG could have a potential for treating neurotoxicity induced by fluoride or related environmental pollutants.
authors.affiliation: Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Seyed Fazel
authors.initials: SF
authors.lastname: Nabavi
authors.forename: Seyed Mohammad
authors.initials: SM
authors.lastname: Nabavi
authors.forename: Solomon
authors.initials: S
authors.lastname: Habtemariam
authors.forename: Akbar Hajizadeh
authors.initials: AH
authors.lastname: Moghaddam
authors.forename: Antoni
authors.initials: A
authors.lastname: Sureda
authors.forename: Morteza
authors.initials: M
authors.lastname: Mirzaei
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology*; D002374:Catalase / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005707:Gallic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D008297:Male; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012969:Sodium Fluoride / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D018696:Neuroprotective Agents; D017392:Thiobarbituric Acid Reactive Substances; C022188:M3OMG; D005707:Gallic Acid; D012969:Sodium Fluoride; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione