title: Immunological complex for enhancement of innate immune response in passive vaccination.
abstract: Passive vaccination is used to treat a wide range of infections and cancer. However, this approach has some limitations. An immune complex termed Y-complex was developed to intensify the effect of the passive vaccine. The complex is composed of a microbead that carries specific antibodies and an inducer. It enables targeting of pathogen or abnormal cells, and stimulation of a desired response by innate immune cells, depending on the inducer. The production and efficacy of Y-complex as a passive immune prophylaxis is demonstrated in this study by its use in treating cow mastitis. In an in vitro assay, Y-complex inhibited propagation and induced phagocytosis of bacteria. In challenge experiments, cows were inoculated through the udder with Escherichia coli or Streptococcus dysgalactiae. Following treatment with Y-complex, no bacteria were isolated in the milk and N-acetyl-ß-D-glucosaminidase activity had returned to normal levels. Thus the Y-complex approach can be used as an effective treatment for mastitis. Due to its modularity, this approach may serve as a treatment for a variety of disease agents.
authors.affiliation: MIGAL-Galilee Technology Center, Kiryat Shmona 11016, Israel.
authors.forename: Elina
authors.initials: E
authors.lastname: Aizenshtein
authors.forename: Yosef
authors.initials: Y
authors.lastname: Pinchasov
authors.forename: Ely
authors.initials: E
authors.lastname: Morag
authors.forename: Gabriel
authors.initials: G
authors.lastname: Leitner
authors.forename: Yossi
authors.initials: Y
authors.lastname: Shpanir
authors.forename: Dorit
authors.initials: D
authors.lastname: Reimond
authors.forename: Jacob
authors.initials: J
authors.lastname: Pitcovski
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex / Q000008:administration & dosage* / Q000276:immunology*; D002417:Cattle; D004926:Escherichia coli / Q000276:immunology; D004927:Escherichia coli Infections / Q000276:immunology / Q000628:therapy / Q000662:veterinary*; D005260:Female; D007113:Immunity, Innate; D007116:Immunization, Passive; D007136:Immunoglobulins / Q000008:administration & dosage / Q000276:immunology; D008321:Mammary Glands, Animal / Q000382:microbiology; D008414:Mastitis, Bovine / Q000276:immunology / Q000628:therapy*; D008863:Microspheres; D008892:Milk / Q000382:microbiology; D013290:Streptococcal Infections / Q000276:immunology / Q000628:therapy / Q000662:veterinary*; D013291:Streptococcus / Q000276:immunology; D016896:Treatment Outcome; D014611:Vaccination
chemical_list: D000936:Antigen-Antibody Complex; D007136:Immunoglobulins