title: A comparative study of antibodies against proteins extracted from staphylococcal biofilm for the diagnosis of orthopedic prosthesis-related infections in an animal model and in humans.
abstract: Staphylococcus aureus and Staphylococcus epidermidis are the microorganisms most frequently seen in periprosthetic infections (PPI) with the capacity of forming biofilm. To find potential antigens for the diagnosis of PPI, the immunogenicity of protein components in biofilm from a model biofilm-positive strain (S. epidermidis RP62A) was investigated. A guinea pig animal model of PPI was developed and sera were obtained. Sera of patients with PPI and those of controls were also collected. Data generated with an enzyme-linked immunosorbent assay showed that there were significantly higher levels of anti-extracellular protein IgG in sera of infected animals than in controls. We also found significantly higher anti-extracellular protein IgG levels in infected patients, compared to the controls; however, receiver operating characteristic curves did not aid in diagnosing PPI.
authors.affiliation: Shanghai Key Laboratory of Orthopaedic Implant, Department of Orthopaedics, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, P.R. China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Wang
authors.forename: Irina
authors.initials: I
authors.lastname: Sadovskaya
authors.forename: Damien
authors.initials: D
authors.lastname: Leterme
authors.forename: Denis
authors.initials: D
authors.lastname: Watier
authors.forename: Ali
authors.initials: A
authors.lastname: Chokr
authors.forename: Zhen'an
authors.initials: Z
authors.lastname: Zhu
authors.forename: Said
authors.initials: S
authors.lastname: Jabbouri
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001426:Bacterial Proteins / Q000276:immunology; D018441:Biofilms; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D019736:Prostheses and Implants / Q000382:microbiology; D016459:Prosthesis-Related Infections / Q000276:immunology / Q000382:microbiology*; D012372:ROC Curve; D013203:Staphylococcal Infections / Q000276:immunology / Q000382:microbiology*; D013212:Staphylococcus epidermidis / Q000276:immunology / Q000502:physiology*
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G