title: Comparison of laccase production levels in Pichia pastoris and Cryptococcus sp. S-2.
abstract: The heterologous expression of the laccase gene from Trametes versicolor and Gaeumannomyces graminis was evaluated in the yeasts Pichia pastoris and Cryptococcus sp. S-2. The expression levels of both laccase genes in Cryptococcus sp. S-2 were considerably higher than those in P. pastoris. The codon usage of Cryptococcus sp. S-2 as well as the GC content were similar to those of T. versicolor and G. graminis. These results suggest that using a host with a similar codon usage for the expressed gene may improve protein expression. The use of Cryptococcus sp. S-2 as a host may be advantageous for the heterologous expression of genes with high GC content. Moreover, this yeast provides the same advantages as P. pastoris for the production of recombinant proteins, such as growth on minimal medium, capacity for high-density growth during fermentation, and capability for post-translational modifications. Therefore, we propose that Cryptococcus sp. S-2 be used as an expression host to improve enzyme production levels when other hosts have not yielded good results.
authors.affiliation: National Research Institute of Brewing, Kagamiyama, Higashi-Hiroshima, Japan.
authors.forename: Nahoko
authors.initials: N
authors.lastname: Nishibori
authors.forename: Kazuo
authors.initials: K
authors.lastname: Masaki
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Tsuchioka
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Fujii
authors.forename: Haruyuki
authors.initials: H
authors.lastname: Iefuji
mesh_terms: D001203:Ascomycota / Q000235:genetics; D003062:Codon; D003454:Cryptococcus / Q000235:genetics* / Q000378:metabolism; D005285:Fermentation; D042845:Laccase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D010843:Pichia / Q000235:genetics* / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals; D011994:Recombinant Proteins / Q000096:biosynthesis; D055454:Trametes / Q000235:genetics
chemical_list: D003062:Codon; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D042845:Laccase