title: A preliminary X-ray study of transketolase from Burkholderia pseudomallei.
abstract: TktA is the most critical enzyme in the nonoxidative pentose phosphate pathway. It catalyzes the conversion of xylulose 5-phosphate and ribose 5-phosphate into sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate, and its products are used in the biosynthesis of acetyl-CoA, aromatic amino acids, nucleic acids and ADP-L-glycero-β-D-manno-heptose. TktA also has an unexpected role in chromosome structure that is independent of its metabolic responsibilities. Therefore, it is a new potent antibiotic target. In this study, TktA from Burkholderia pseudomallei has been cloned, expressed, purified and crystallized. Synchrotron X-ray data were also collected to 2.0 Å resolution. The crystal belonged to the monoclinic space group C2, with unit-cell parameters a=146.2, b=74.6, c=61.6 Å, β=113.0°. A full structural determination is under way in order to provide insight into the structure-function relationship of this protein.
authors.affiliation: The Center for Cell Signaling and Drug Discovery Research, College of Pharmacy, Division of Life and Pharmaceutical Sciences, Ewha Womans University, Seoul 120-750, Republic of Korea.
authors.forename: Mi Sun
authors.initials: MS
authors.lastname: Kim
authors.forename: Areum
authors.initials: A
authors.lastname: Lim
authors.forename: Seung Won
authors.initials: SW
authors.lastname: Yang
authors.forename: Daeun
authors.initials: D
authors.lastname: Lee
authors.forename: Jimin
authors.initials: J
authors.lastname: Park
authors.forename: Dong Hae
authors.initials: DH
authors.lastname: Shin
mesh_terms: D016957:Burkholderia pseudomallei / Q000201:enzymology* / Q000378:metabolism; D018360:Crystallography, X-Ray; D013329:Structure-Activity Relationship; D013403:Sugar Phosphates / Q000737:chemistry / Q000378:metabolism; D014174:Transketolase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D013403:Sugar Phosphates; C020495:sedoheptulose 7-phosphate; D014174:Transketolase