title: Development of acolorimetric, superparamagnetic biosensor for the capture and detection of biomolecules.
abstract: Polydiacetylenes are ideal sensing materials due to their ability to change color in response to external stimuli. Here we report a new colorimetric, magnetically separable biosensor using a diacetylene monomer modified with a hydrophilic lysine derivative and superparamagnetic iron oxide nanoparticles. The particles demonstrated a distinct blue to red color transition when added to ethanol and could be quickly separated from solution with a common magnet. Surface functionalization of the particles with bovine serum albumin (BSA) showed the ability to capture and detect anti-BSA antibody from antiserum with a colorimetric response, demonstrating our biosensors can serve as a platform for diagnostic applications including point-of-care testing.
authors.affiliation: Department of Chemical Engineering, University of Waterloo, Waterloo, Ontario N2L3G1, Canada.
authors.forename: Terence
authors.initials: T
authors.lastname: Chan
authors.forename: Frank
authors.initials: F
authors.lastname: Gu
mesh_terms: D000906:Antibodies / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D003124:Colorimetry; D003911:Dextrans / Q000737:chemistry; D007106:Immune Sera / Q000032:analysis / Q000276:immunology; D060328:Magnetic Phenomena; D058185:Magnetite Nanoparticles / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry* / Q000276:immunology; D012710:Serum Albumin, Bovine
chemical_list: D000906:Antibodies; D003911:Dextrans; D007106:Immune Sera; D058185:Magnetite Nanoparticles; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; D012710:Serum Albumin, Bovine; C082361:polydiacetylene; C065507:ferumoxides