title: Resveratrol pre-treatment reduces early inflammatory responses induced by status epilepticus via mTOR signaling.
abstract: Resveratrol is indicated to be involved in neuroprotection and anti-inflammation in epileptic rats. The molecular mechanism is still not fully understood. In this study, we investigated the role of resveratrol in nuclear factor-kappa B associated inflammatory responses induced by status epilepticus. Our data showed that seizures activated mammalian target of rapamycin (mTOR), increased the activity of nuclear factor-kappa B and promoted the expressions of inflammatory molecules including inducible nitric oxide synthase, cyclooxygenase-2 and interleukin-1β. Futhermore, resveratrol significantly inhibited the activation of nuclear factor-kappa B and the production of proinflammatory molecules via mTOR pathway. Additionally, we also proved that the inhibition of mTOR signal by resveratrol was mostly attributed to AMP-activated kinase (AMPK) activation. Altogether, our results suggest that resveratrol suppresses inflammatory responses induced by seizures partially via AMPK/mTOR pathway.
authors.affiliation: Department of Neurology, Qilu Hospital, Shandong University, 107#, Wenhua Xi Road, Ji'nan 250012, PR China.
authors.forename: Sheng-Jun
authors.initials: SJ
authors.lastname: Wang
authors.forename: Qi-Yu
authors.initials: QY
authors.lastname: Bo
authors.forename: Xiu-He
authors.initials: XH
authors.lastname: Zhao
authors.forename: Xue
authors.initials: X
authors.lastname: Yang
authors.forename: Zhao-Fu
authors.initials: ZF
authors.lastname: Chi
authors.forename: Xue-Wu
authors.initials: XW
authors.lastname: Liu
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D015153:Blotting, Western; D024202:Electrophoretic Mobility Shift Assay; D004797:Enzyme-Linked Immunosorbent Assay; D006624:Hippocampus / Q000378:metabolism; D007249:Inflammation / Q000209:etiology* / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D000077185:Resveratrol; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013226:Status Epilepticus / Q000150:complications / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology*; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D013267:Stilbenes; C546845:mTOR protein, rat; D058570:TOR Serine-Threonine Kinases; D000077185:Resveratrol