title: Isolation and characterization of microsatellite loci in a polyploid alpine herb, Callianthemum miyabeanum (Ranunculaceae).
abstract: OBJECTIVE
  Nuclear microsatellite primers were developed to analyze the clonal diversity and population genetic structure of the endemic polyploid herb Callianthemum miyabeanum. •
  
  
  RESULTS
  Using a protocol for constructing microsatellite-enriched libraries, 15 primer sets were developed for use in C. miyabeanum. The number of alleles found ranged from five to 22. The estimated range of expected heterozygosities was 0.574 to 0.907, and the Shannon-Weiner diversity index ranged from 1.061 to 2.733. Cross-amplification of all loci was also successful in the closely related endemic species C. kirigishiense and C. hondoense. •
  
  
  CONCLUSIONS
  The development of these microsatellite loci will facilitate a deeper understanding of the genetic diversity, mode of reproduction, and population structure of not only C. miyabeanum, but also the other Callianthemum species endemic to Japan.
authors.affiliation: Field Center for Sustainable Agriculture and Forestry, Faculty of Agriculture, Niigata University, 94-2 Koda, Sado, Niigata 952-2206, Japan. habe@agr.niigata-u.ac.jp
authors.forename: Harue
authors.initials: H
authors.lastname: Abe
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nishikawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Shimamura
authors.forename: Ken
authors.initials: K
authors.lastname: Sato
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Suyama
mesh_terms: D002467:Cell Nucleus / Q000235:genetics; D017931:DNA Primers / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D056727:Endangered Species; D007564:Japan; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D029626:Ranunculaceae / Q000166:cytology / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018744:DNA, Plant