title: Effects of low-density lipoproteins extracted from different avian yolks on boar spermatozoa quality following freezing-thawing.
abstract: Low-density lipoproteins (LDL) is known to protect boar sperm during freezing-thawing, but little information is known about the effects of LDL extracted from different avian egg yolks on post-thaw boar semen quality. The purpose of this study was to compare and analyze the effects of LDL at various concentrations and different species on boar sperm quality after freezing-thawing. LDL extracted from the yolk of hen egg, duck egg, quail egg, pigeon egg or ostrich egg was added to the extender at the concentrations of 0.06, 0.07, 0.08, 0.09 and 0.1 g/ml, respectively, and their effects on frozen-thawed boar sperm quality were assessed. According to all measured parameters, the results showed that sperm motility, acrosome integrity and plasma membrane integrity were 43.20%, 52.57% and 48.13%, respectively, after being frozen-thawed with 0.09 g/ml LDL extracted from pigeon egg yolk. All these quality parameters were higher than that of other groups (P < 0.05). In conclusion, our results confirmed that LDL extracted from pigeon egg yolk had the best cryoprotective effects on frozen-thawed boar sperm among all of the groups supplemented with LDL from five kinds of avian egg in extender. The optimum concentration of LDL extracted from pigeon egg in boar semen freezing extender was 0.09 g/ml.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
authors.forename: Yan-Feng
authors.initials: YF
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
authors.forename: Chun-Wei
authors.initials: CW
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
authors.forename: Shu-Hai
authors.initials: SH
authors.lastname: Bu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Hu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
authors.forename: Qing-Wang
authors.initials: QW
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Pang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
authors.forename: Gong-She
authors.initials: GS
authors.lastname: Yang
mesh_terms: D000177:Acrosome / Q000187:drug effects*; D000818:Animals; D002478:Cells, Cultured; D015925:Cryopreservation; D004530:Egg Yolk / Q000737:chemistry*; D005615:Freezing; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008297:Male; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000166:cytology* / Q000502:physiology*; D013552:Swine
chemical_list: D008077:Lipoproteins, LDL