title: Plant Regeneration with Callus and Protoplasts of Solanum uporo Dun.
abstract: Vigorous plants exceding 1 m in height and producing fruits with seeds have been regenerated from callus derived from hypocotyl segments in less than one year, using B5-media containing 0.5 mg · l(-1) 2,4-D, 0.25 mg · l(-1) kinetin, and 0.5 g · l(-1) casein hydrolyzate for callus formation, MS-media with 0.005 mg · l(-1) NAA and 0.5 mg · l(-1) zeatin for shoot formation, and MS-media with 0.005 mg · l(-1) NAA for rooting and plantlet formation. Of cytokinins tested zeatin was the most effective. Protoplasts from leaves of regenerated shoots cultured in vitro divided and formed callus which again and reliably produced shoots and plantlets when placed on MS-media as specified before.
authors.affiliation: Plant Biotechnology Institute, National Research Council, Saskatoon, Saskatchewan, S7N 0W9 Canada.
authors.forename: G G
authors.initials: GG
authors.lastname: Li
authors.forename: F
authors.initials: F
authors.lastname: Constabel