title: Equine sarcoids: Bovine Papillomavirus type 1 transformed fibroblasts are sensitive to cisplatin and UVB induced apoptosis and show aberrant expression of p53.
abstract: Bovine papillomavirus type 1 infects not only cattle but also equids and is a causative factor in the pathogenesis of commonly occurring equine sarcoid tumours. Whilst treatment of sarcoids is notoriously difficult, cisplatin has been shown to be one of the most effective treatment strategies for sarcoids. In this study we show that in equine fibroblasts, BPV-1 sensitises cells to cisplatin-induced and UVB-induced apoptosis, a known cofactor for papillomavirus associated disease, however BPV-1 transformed fibroblasts show increased clonogenic survival, which may potentially limit the therapeutic effects of repeated cisplatin treatment. Furthermore we show that BPV-1 increases p53 expression in sarcoid cell lines and p53 expression can be either nuclear or cytoplasmic. The mechanism and clinical significance of increase/abnormal p53 expression remains to be established.
authors.affiliation: MRC-University of Glasgow Centre for Virus Research, Institute of Infection, Inflammation and Immunity, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden Road, Glasgow, Scotland, G61 1QH, United Kingdom. Lubna.Nasir@gla.ac.uk.
authors.forename: Margaret
authors.initials: M
authors.lastname: Finlay
authors.forename: Zhengqiang
authors.initials: Z
authors.lastname: Yuan
authors.forename: Iain M
authors.initials: IM
authors.lastname: Morgan
authors.forename: M Saveria
authors.initials: MS
authors.lastname: Campo
authors.forename: Lubna
authors.initials: L
authors.lastname: Nasir
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western / Q000662:veterinary; D052688:Bovine papillomavirus 1 / Q000502:physiology*; D002945:Cisplatin / Q000008:administration & dosage / Q000494:pharmacology*; D005347:Fibroblasts / Q000821:virology; D005786:Gene Expression Regulation; D006734:Horse Diseases / Q000235:genetics / Q000821:virology*; D006736:Horses; D030361:Papillomavirus Infections / Q000235:genetics / Q000662:veterinary* / Q000821:virology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D000970:Antineoplastic Agents; D016159:Tumor Suppressor Protein p53; D002945:Cisplatin