title: Subcellular localization of the epitheliopeptide, Hym-301, in Hydra.
abstract: Peptides, as signaling molecules, play a number of roles in cell activities. An epitheliopeptide, Hym-301, has been described as a peptide involved in morphogenesis in hydra. However, little is known about the intracellular location of the peptide or its specific functions. To investigate the mechanism of morphogenesis that involves peptidic molecules, we have examined the intracellular localization of Hym-301 in hydra by using immunohistochemical and immunogold electron-microscopic analyses. We have found that the pattern of distribution of mature peptide is slightly different from that of its mRNA, and that the peptide is stored in vesicles located adjacent to the cell membrane. We have also found that the peptide is released both extracellularly and internally to the cytoplasm of the cells. Based upon these observations, we have constructed a possible model mechanism of homeostatic regulation of the distribution of the Hym-301 peptide in a dynamic tissue context.
authors.affiliation: Department of Biology, Hamamatsu University School of Medicine, Hamamatsu, 431-3192, Japan. ytakaku@hama-med.ac.jp
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Takaku
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimizu
authors.forename: Toshio
authors.initials: T
authors.lastname: Takahashi
authors.forename: Toshitaka
authors.initials: T
authors.lastname: Fujisawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D005786:Gene Expression Regulation; D006829:Hydra / Q000378:metabolism* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D010455:Peptides; D012333:RNA, Messenger