title: Acid and alkali etching of grit blasted zirconia: Impact on adhesion and osteogenic differentiation of MG63 cells in vitro.
abstract: There is a need for evaluating zirconia surface modifications and their potential impact on the biological response of osteogenic cells. Grit blasted zirconia discs were either left untreated or underwent acid or alkaline etching. Adhesion and osteogenic differentiation of MG63 cells was determined after one week of culture. The macro-scaled roughness of the grit blasted zirconia discs, independent of the surface treatment, was within a narrow range and only slightly smoother than titanium discs. However, the alkaline- and acid-etching led to an increase of the micro-roughness of the surface. The surface modifications had no effect on cell spreading and did not cause significant change in the expression of differentiation markers. Thus, in this respective setting, morphologic changes observed upon treatment of grit blasted zirconia discs with acid or alkaline do not translate into changes in MG63 cell adhesion or differentiation and are comparable to findings with anodized titanium discs.
authors.affiliation: Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Bern, Bern, Switzerland. reinhard.gruber@zmk.unibe.ch
authors.forename: Reinhard
authors.initials: R
authors.lastname: Gruber
authors.forename: Erik
authors.initials: E
authors.lastname: Hedbom
authors.forename: Dieter D
authors.initials: DD
authors.lastname: Bosshardt
authors.forename: Roman
authors.initials: R
authors.lastname: Heuberger
authors.forename: Daniel
authors.initials: D
authors.lastname: Buser
mesh_terms: D000143:Acids; D000468:Alkalies; D000704:Analysis of Variance; D002448:Cell Adhesion; D002454:Cell Differentiation; D045662:Dental Etching / Q000379:methods; D006801:Humans; D008422:Materials Testing; D010006:Osteoblasts; D010012:Osteogenesis; D013499:Surface Properties; D015040:Zirconium
chemical_list: D000143:Acids; D000468:Alkalies; D015040:Zirconium; C028541:zirconium oxide