title: Nanocolloidal gold-based immuno-dip strip assay for rapid detection of Sudan red I in food samples.
abstract: A semiquantitative dip strip assay was developed using nanocolloidal gold-labelled monoclonal antibody (Mab) 8A10 for the rapid detection of Sudan red I in food samples. A protein-Sudan red I conjugate was coated on a nitro cellulose membrane strip in a defined test line. In flow of the complex of nanocolloidal gold labelled-Mab and Sudan red I along the strip, intensive red colour that was formed in the test line reflected the Sudan red I concentration. The test required 10 min and had a visual limit of detection of 10 ng/g Sudan red I in tomato sauce and chilli powder samples. The results of the strip assay agreed well with those of a high performance liquid chromatography method for both spiked and real commercial samples. The strip was stable for at least 2 months at 4°C. The strip assay offers the potential as a useful rapid and simple method for screening of Sudan red I in food samples.
authors.affiliation: College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.forename: Zhanhui
authors.initials: Z
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Qing X
authors.initials: QX
authors.lastname: Li
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.forename: Ting
authors.initials: T
authors.lastname: Xu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology; D001391:Azo Compounds / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D005260:Female; D005506:Food Contamination / Q000032:analysis*; D017979:Gold Colloid / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D017365:Spices / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D001391:Azo Compounds; D017979:Gold Colloid; C033003:sudan red