title: The origin of stroma surrounding epithelial ovarian cancer cells.
abstract: Cancer stroma is thought to play an important role in tumor behavior, including invasion or metastasis and response to therapy. Cancer stroma is generally thought either to be non-neoplastic cells, including tissue-marrow or bone-marrow-derived fibroblasts, or to originate in epithelial mesenchymal transition of cancer cells. In this study, we evaluated the status of the p53 gene in both the cancer cells and the cancer stroma in epithelial ovarian cancer (EOC) to elucidate the origin of the stroma. Samples from 16 EOC patients were included in this study. Tumor cells and adjacent nontumor stromal cells were microdissected and DNA was extracted separately. We analyzed p53 sequences (exons 5-8) of both cancer and stromal tissues in all cases. Furthermore, we examined p53 protein expression in all cases. Mutations in p53 were detected in 9 of the 16 EOCs: in 8 of these cases, the mutations were detected only in cancer cells. In 1 case, the same mutation (R248Q) was detected in both cancer and stromal tissues, and p53 protein expression was detected in both the cancer cells and the cancer stroma. Most cancer stroma in EOC is thought to originate from non-neoplastic cells, but some parts of the cancer stroma might originate from cancer cells.
authors.affiliation: Department of Obstetrics and Gynecology, Keio University, Osaka, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Akahane
authors.forename: Akira
authors.initials: A
authors.lastname: Hirasawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsuda
authors.forename: Fumio
authors.initials: F
authors.lastname: Kataoka
authors.forename: Sadako
authors.initials: S
authors.lastname: Nishimura
authors.forename: Hideo
authors.initials: H
authors.lastname: Tanaka
authors.forename: Eiichiro
authors.initials: E
authors.lastname: Tominaga
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nomura
authors.forename: Tatsuyuki
authors.initials: T
authors.lastname: Chiyoda
authors.forename: Yoko
authors.initials: Y
authors.lastname: Iguchi
authors.forename: Wataru
authors.initials: W
authors.lastname: Yamagami
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Susumu
authors.forename: Daisuke
authors.initials: D
authors.lastname: Aoki
mesh_terms: D000328:Adult; D000368:Aged; D000077216:Carcinoma, Ovarian Epithelial; D003238:Connective Tissue / Q000378:metabolism* / Q000473:pathology; D005260:Female; D016158:Genes, p53; D006801:Humans; D007150:Immunohistochemistry; D061346:Laser Capture Microdissection; D008875:Middle Aged; D009154:Mutation; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D059016:Tumor Microenvironment; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D016159:Tumor Suppressor Protein p53