title: Integration of first and second generation biofuels: fermentative hydrogen production from wheat grain and straw.
abstract: Integrating of lignocellulose-based and starch-rich biomass-based hydrogen production was investigated by mixing wheat straw hydrolysate with a wheat grain hydrolysate for improved fermentation. Enzymatic pretreatment and hydrolysis of wheat grains led to a hydrolysate with a sugar concentration of 93.4 g/L, while dilute-acid pretreatment and enzymatic hydrolysis of wheat straw led to a hydrolysate with sugar concentration 23.0 g/L. Wheat grain hydrolysate was not suitable for hydrogen production by the extreme thermophilic bacterium Caldicellulosiruptor saccharolyticus at glucose concentrations of 10 g/L or higher, and wheat straw hydrolysate showed good fermentability at total sugar concentrations of up to 10 g/L. The mixed hydrolysates showed good fermentability at the highest tested sugar concentration of 20 g/L, with a hydrogen production of 82-97% of that of the control with pure sugars. Mixing wheat grain hydrolysate with wheat straw hydrolysate would be beneficial for fermentative hydrogen production in a biorefinery.
authors.affiliation: Wageningen UR Food & Biobased Research, PO Box 17, 6700 AA Wageningen, The Netherlands. ioannis.panagiotopoulos@ubc.ca
authors.forename: I A
authors.initials: IA
authors.lastname: Panagiotopoulos
authors.forename: R R
authors.initials: RR
authors.lastname: Bakker
authors.forename: T
authors.initials: T
authors.lastname: de Vrije
authors.forename: P A M
authors.initials: PA
authors.lastname: Claassen
authors.forename: E G
authors.initials: EG
authors.lastname: Koukios
mesh_terms: D056804:Biofuels / Q000382:microbiology*; D005285:Fermentation; D006859:Hydrogen / Q000302:isolation & purification* / Q000378:metabolism*; D035261:Plant Components, Aerial / Q000382:microbiology*; D018511:Systems Integration; D046968:Thermoanaerobacter / Q000378:metabolism*; D014908:Triticum / Q000382:microbiology*
chemical_list: D056804:Biofuels; D006859:Hydrogen