title: Synthesis and characterization of the 47-residue heterodimeric antimicrobial peptide distinctin, featuring directed disulfide bridge formation.
abstract: Distinctin, a 47-residue heterodimeric peptide with potent antimicrobial activity, comprises two monomeric units linked covalently by a disulfide bond between Cys19 from the 22-residue A chain and Cys23 from the 25-residue B chain. Previous synthetic strategies involved assemblies of the two individual chains, followed by their co-oxidation to form the connecting disulfide bridge, and resulted in a mixture of three species: two homodimers and one heterodimer. Here, we report synthesis of exclusively heterodimeric distinctin, using recently developed tactics for directed disulfide bridge formation. Material prepared this way was characterized and found to be suitable for more detailed structural studies.
authors.affiliation: Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA.
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Mullen
authors.forename: Raffaello
authors.initials: R
authors.lastname: Verardi
authors.forename: Fernando
authors.initials: F
authors.lastname: Porcelli
authors.forename: Andrea
authors.initials: A
authors.lastname: Scaloni
authors.forename: George
authors.initials: G
authors.lastname: Barany
authors.forename: Gianluigi
authors.initials: G
authors.lastname: Veglia
mesh_terms: D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000138:chemical synthesis* / Q000737:chemistry*; D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis* / Q000737:chemistry*; D060326:Chemistry Techniques, Synthetic; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000737:chemistry; D004220:Disulfides / Q000138:chemical synthesis / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D055503:Protein Multimerization
chemical_list: D029845:Amphibian Proteins; D023181:Antimicrobial Cationic Peptides; D004220:Disulfides; C531945:distinctin; D003545:Cysteine