title: Detection of single-nucleotide polymorphisms based on the formation of an electron-transfer impeding layer on an electrode surface.
abstract: An electrochemical biosensor utilizing the electron-transfer impeding (insulating) power of a conducting polymer for label-free genotyping single-nucleotide polymorphisms is proposed. Excellent selectivity and sensitivity are accomplished through the engagement of a nuclease clean-up step and a cumulative signal generation/amplification process, respectively.
authors.affiliation: Department of Chemistry, National University of Singapore, 117543, Singapore. chmgaoz@nus.edu.sg
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.forename: Huimin
authors.initials: H
authors.lastname: Deng
authors.forename: Yuqian
authors.initials: Y
authors.lastname: Ren
mesh_terms: D015374:Biosensing Techniques; D017097:Electric Impedance; D004566:Electrodes; D004579:Electron Transport; D005821:Genetic Techniques / Q000592:standards*; D020641:Polymorphism, Single Nucleotide; D013499:Surface Properties