title: Using a fiber optic particle plasmon resonance biosensor to determine kinetic constants of antigen-antibody binding reaction.
abstract: In this paper, one simple and label-free biosensing method has been developed for determining the binding kinetic constants of antiovalbumin antibody (anti-OVA) and anti-mouse IgG antibody using the fiber optic particle plasmon resonance (FOPPR) biosensor. The FOPPR sensor is based on gold-nanoparticle-modified optical fiber, where the gold nanoparticle surface has been modified by a mixed self-assembled monolayer for conjugation of a molecular probe reporter (ovalbumin or mouse IgG) to dock with the corresponding analyte species such as anti-OVA or anti-mouse IgG. The binding process, occurring when an analyte reacts with a probe molecule immobilized on the optical fiber, can be monitored in real-time. In addition, by assuming a Langmuir-type adsorption isotherm to measure the initial binding rate, the quantitative determination of binding kinetic constants, the association and dissociation rate constants, yields k(a) of (7.21 ± 0.4) × 10(3) M(-1) s(-1) and k(d) of (2.97 ± 0.1) × 10(-3) s(-1) for OVA/anti-OVA and k(a) of (1.45 ± 0.2) × 10(6) M(-1) s(-1) and k(d) of (2.97 ± 0.6) × 10(-2) s(-1) for mouse IgG/anti-mouse IgG. We demonstrate that the FOPPR biosensor can study real-time biomolecular interactions.
authors.affiliation: Department of Chemistry and Biochemistry, National Chung Cheng University, Min-Hsiung, Chia-Yi, Taiwan.
authors.forename: Ting-Chou
authors.initials: TC
authors.lastname: Chang
authors.forename: Chao-Ching
authors.initials: CC
authors.lastname: Wu
authors.forename: Shau-Chun
authors.initials: SC
authors.lastname: Wang
authors.forename: Lai-Kwan
authors.initials: LK
authors.lastname: Chau
authors.forename: Wen-Hsin
authors.initials: WH
authors.lastname: Hsieh
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D000915:Antibody Affinity; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques; D006046:Gold / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology*; D007700:Kinetics; D053768:Metal Nanoparticles / Q000737:chemistry; D051379:Mice; D055100:Optical Fibers; D010047:Ovalbumin / Q000276:immunology*; D020349:Surface Plasmon Resonance
chemical_list: D055910:Antibodies, Immobilized; D007074:Immunoglobulin G; D006046:Gold; D010047:Ovalbumin