title: Effects of acetylcholine on an animal mode of inflammation.
abstract: Acetylcholinesterase (AChE) is found both on the membranes of neuronal and non-neuronal cells. In this study we performed intravenous administrations of velnacrine (VLN) and acetylcholine (ACh), respectively, AChE inhibitor and substrate, in an animal model of lipopolysaccharide (LPS)-induced inflammation in Wistar rats. Using intravital microscopy the number of rolling and adherent leukocytes in post-capillary venules was monitorized and blood samples were collected for TNF-α plasma concentrations determination. Our results showed that in presence of LPS, ACh has an anti-inflammatory effect, seen by a decrease in TNF-α plasma levels and maintains the number of rolling and adherent leukocytes. The presence of VLN before LPS almost blocked the LPS-induced rolling and TNF-α releasing. Thereby VLN seems to have, like ACh, an anti-inflammatory effect by diminishing TNF-α concentrations.
authors.affiliation: Institute of Biochemistry, Institute of Molecular Medicine, University of Lisbon Medical School, Lisbon, Portugal. anarmsilva@fm.ul.pt
authors.forename: A S
authors.initials: AS
authors.lastname: Silva-Herdade
authors.forename: C
authors.initials: C
authors.lastname: Saldanha
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000187:drug effects; D007249:Inflammation / Q000188:drug therapy*; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology*; D008070:Lipopolysaccharides; D008297:Male; D008853:Microscopy; D051381:Rats; D017208:Rats, Wistar; D013619:Tacrine / Q000031:analogs & derivatives / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D002800:Cholinesterase Inhibitors; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D013619:Tacrine; D000109:Acetylcholine; C056424:velnacrine