title: Transcription factor Spi-B-dependent and -independent pathways for the development of Peyer's patch M cells.
abstract: Although many of the biological features of microfold cells (M cells) have been known for many years, the molecular mechanisms of M-cell development and antigen recognition have remained unclear. Here, we report that Umod is a novel M-cell-specific gene, the translation products of which might contribute to the uptake function of M cells. Transcription factor Spi-B was also specifically expressed in M cells among non-hematopoietic lineages. Spi-B-deficient mice showed reduced expression of most, but not all, other M-cell-specific genes and M-cell surface markers. Whereas uptake of Salmonella Typhimurium via M cells was obviously reduced in Spi-B-deficient mice, the abundance of intratissue cohabiting bacteria was comparable between wild-type and Spi-B-deficient mice. These data indicate that there is a small M-cell population with developmental regulation that is Spi-B independent; however, Spi-B is probably a candidate master regulator of M-cell functional maturation and development by another pathway.
authors.affiliation: Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Sato
authors.forename: S
authors.initials: S
authors.lastname: Kaneto
authors.forename: N
authors.initials: N
authors.lastname: Shibata
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Okura
authors.forename: Y
authors.initials: Y
authors.lastname: Yuki
authors.forename: J
authors.initials: J
authors.lastname: Kunisawa
authors.forename: H
authors.initials: H
authors.lastname: Kiyono
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation; D058851:GPI-Linked Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D051379:Mice; D018345:Mice, Knockout; D008871:Microvilli / Q000378:metabolism; D009928:Organ Specificity / Q000235:genetics; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D050783:Proto-Oncogene Proteins c-ets / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D058949:Uromodulin / Q000235:genetics / Q000378:metabolism
chemical_list: D000954:Antigens, Surface; D058851:GPI-Linked Proteins; C495998:Gp2 protein, mouse; D050783:Proto-Oncogene Proteins c-ets; C514177:Spi-B protein, mouse; C548613:Umod protein, mouse; D058949:Uromodulin