title: Multiplex protein detection with DNA readout via mass spectrometry.
abstract: Multiplex protein quantification has been constrained by issues of assay specificity, sensitivity and throughput. This research presents a novel approach that overcomes these limitations using antibody-oligonucleotide conjugates for immuno-polymerase chain reaction (immuno-PCR) or proximity ligation, coupled with competitive PCR and MALDI-TOF mass spectrometry. Employing these combinations of technologies, we demonstrate multiplex detection and quantification of up to eight proteins, spanning wide dynamic ranges from femtomolar concentrations, using only microliter sample volumes.
authors.affiliation: Center for Advanced Biotechnology, Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA. jflanigon@gmail.com
authors.forename: James
authors.initials: J
authors.lastname: Flanigon
authors.forename: Masood
authors.initials: M
authors.lastname: Kamali-Moghaddam
authors.forename: Ian
authors.initials: I
authors.lastname: Burbulis
authors.forename: Carla
authors.initials: C
authors.lastname: Annink
authors.forename: Martin
authors.initials: M
authors.lastname: Steffen
authors.forename: Paul
authors.initials: P
authors.lastname: Oeth
authors.forename: Roger
authors.initials: R
authors.lastname: Brent
authors.forename: Dirk
authors.initials: D
authors.lastname: van den Boom
authors.forename: Ulf
authors.initials: U
authors.lastname: Landegren
authors.forename: Charles
authors.initials: C
authors.lastname: Cantor
mesh_terms: D000818:Animals; D002645:Chickens; D004247:DNA / Q000032:analysis*; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D011506:Proteins; D004247:DNA