title: The role of immunohistochemistry in the diagnosis of neoplastic pleural effusions.
abstract: Identification of the origin of neoplastic pleural effusion is a major concern in lung pathology. This study followed the diagnostic role of a panel of antibodies that included calretinin, HBME1, D2-40, Ber-EP4, CK5/6, CEA and TTF1, in a total of 37 cases of pleural and lung cancer with tumor-type cytology, later confirmed by histopathology. For mesothelioma, positive staining for calretinin, D2-40 and CK5/6 and negative for CEA and TTF1 were characteristic. For lung adenocarcinomas, we found Ber-EP4, CEA and TTF1 positivity, and calretinin, D2-40 and CK5/6 negativity. Squamous lung carcinomas were positive for Ber-EP4, CK5/6 and CEA and negative for HBME1, D2-40 and TTF1. The panel of antibodies used in this study provides a differential diagnosis between mesotheliomas and lung carcinomas as well as between lung adenocarcinomas and squamous carcinomas.
authors.affiliation: Department of Pathology, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 2-4 Petru Rareş Street, Craiova, Romania.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Dinu
authors.forename: Raluca Niculina
authors.initials: RN
authors.lastname: Ciurea
authors.forename: Mirela
authors.initials: M
authors.lastname: Stefan
authors.forename: Ana Claudia
authors.initials: AC
authors.lastname: Georgescu
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis; D003581:Cytodiagnosis / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D016066:Pleural Effusion, Malignant / Q000175:diagnosis* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor