title: Using hydrogen/deuterium exchange mass spectrometry to define the specific interactions of the phospholipase A2 superfamily with lipid substrates, inhibitors, and membranes.
abstract: The phospholipase A(2) (PLA(2)) superfamily consists of 16 groups and many subgroups and constitutes a diverse set of enzymes that have a common catalytic activity due to convergent evolution. However, different PLA(2) types have unique three-dimensional structures and catalytic residues as well as specific tissue localization and distinct biological functions. Understanding how the different PLA(2) enzymes associate with phospholipid membranes, specific phospholipid substrate molecules, and inhibitors on a molecular basis has advanced in recent years due to the introduction of hydrogen/deuterium exchange mass spectrometry. Its theory, practical considerations, and application to understanding PLA(2)/membrane interactions are addressed.
authors.affiliation: Department of Chemistry and Biochemistry and Department of Pharmacology, University of California, San Diego, La Jolla, California 92093-0601, USA.
authors.forename: Jian
authors.initials: J
authors.lastname: Cao
authors.forename: John E
authors.initials: JE
authors.lastname: Burke
authors.forename: Edward A
authors.initials: EA
authors.lastname: Dennis
mesh_terms: D000495:Allosteric Site; D001665:Binding Sites; D020134:Catalytic Domain; D002462:Cell Membrane / Q000378:metabolism*; D003903:Deuterium; D041961:Deuterium Exchange Measurement; D006801:Humans; D006859:Hydrogen; D013058:Mass Spectrometry / Q000379:methods*; D008958:Models, Molecular; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D064801:Phospholipase A2 Inhibitors; D054467:Phospholipases A2 / Q000737:chemistry / Q000378:metabolism*; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D010726:Phosphodiesterase Inhibitors; D064801:Phospholipase A2 Inhibitors; D010743:Phospholipids; D006859:Hydrogen; D003903:Deuterium; D054467:Phospholipases A2