title: Near-infrared optical guided surgery of highly infiltrative fibrosarcomas in cats using an anti-αvß3 integrin molecular probe.
abstract: We investigated how near-infrared imaging could improve highly infiltrative spontaneous fibrosarcoma surgery in 12 cats in a clinical veterinary phase. We used an RGD-based nanoprobe at different doses and times before surgery and a portable clinical grade imaging system. All tumours were labelled by the tracer and had an overall tumour-to-healthy tissue ratio of 14±1 during surgery. No false negatives were found, and the percentage of tumour cells was linearly correlated with the fluorescence intensity. All cats recovered well and were submitted to long-term follow-up that is currently on-going 1year after the beginning of the study.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 823, Institut Albert Bonniot, Grenoble, France; Université Joseph Fourier (UJF), Grenoble, France.
authors.forename: Christiane H F
authors.initials: CH
authors.lastname: Wenk
authors.forename: Frédérique
authors.initials: F
authors.lastname: Ponce
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Guillermet
authors.forename: Corinne
authors.initials: C
authors.lastname: Tenaud
authors.forename: Didier
authors.initials: D
authors.lastname: Boturyn
authors.forename: Pascal
authors.initials: P
authors.lastname: Dumy
authors.forename: Dorothée
authors.initials: D
authors.lastname: Watrelot-Virieux
authors.forename: Claude
authors.initials: C
authors.lastname: Carozzo
authors.forename: Véronique
authors.initials: V
authors.lastname: Josserand
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Coll
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000378:metabolism / Q000601:surgery*; D002415:Cats; D005260:Female; D005354:Fibrosarcoma / Q000378:metabolism / Q000532:radiotherapy / Q000601:surgery / Q000662:veterinary*; D005455:Fluorescent Antibody Technique / Q000379:methods / Q000662:veterinary; D007089:Image Enhancement / Q000379:methods; D039302:Integrin alphaVbeta3 / Q000032:analysis* / Q000378:metabolism; D008297:Male; D015335:Molecular Probes; D019265:Spectroscopy, Near-Infrared / Q000379:methods / Q000662:veterinary; D014018:Tissue Distribution
chemical_list: D039302:Integrin alphaVbeta3; D015335:Molecular Probes