title: Biocatalytic synthesis of poly(δ-valerolactone) using a thermophilic esterase from archaeoglobus fulgidus as catalyst.
abstract: The ring-opening polymerization of δ-valerolactone catalyzed by a thermophilic esterase from the archaeon Archaeoglobus fulgidus was successfully conducted in organic solvents. The effects of enzyme concentration, temperature, reaction time and reaction medium on monomer conversion and product molecular weight were systematically evaluated. Through the optimization of reaction conditions, poly(δ-valerolactone) was produced in 97% monomer conversion, with a number-average molecular weight of 2225 g/mol, in toluene at 70 °C for 72 h. This paper has produced a new biocatalyst for the synthesis of poly(δ-valerolactone), and also deeper insight has been gained into the mechanism of thermophilic esterase-catalyzed ring-opening polymerization.
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, Changchun 130012, China. yyan@jlu.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Cao
authors.forename: Haobo
authors.initials: H
authors.lastname: Han
authors.forename: Guangquan
authors.initials: G
authors.lastname: Li
authors.forename: Jiebing
authors.initials: J
authors.lastname: Yang
authors.forename: Lingfei
authors.initials: L
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Xuedong
authors.initials: X
authors.lastname: Fang
authors.forename: Quanshun
authors.initials: Q
authors.lastname: Li
mesh_terms: D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism*; D019611:Archaeoglobus fulgidus / Q000201:enzymology*; D055162:Biocatalysis; D004950:Esterases / Q000235:genetics / Q000378:metabolism*; D008970:Molecular Weight; D058105:Polymerization; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D011753:Pyrones / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D013696:Temperature; D013997:Time Factors; D014050:Toluene / Q000737:chemistry
chemical_list: D019843:Archaeal Proteins; D011108:Polymers; D011753:Pyrones; D011994:Recombinant Proteins; C052207:delta-valerolactone; D014050:Toluene; D004950:Esterases