title: Influence of HIV-associated degree of immune suppression on molecular heterogeneity of hepatitis B virus among HIV co-infected patients.
abstract: We have investigated the molecular diversity of Hepatitis B virus (HBV) among the HIV co-infected patients from eastern-India. HBsAg/HBV-DNA positive subjects (n=73) from 874 HIV-infected patients were analyzed by sequencing followed by genetic diversity quantification. HBV/genotype-D and HBV/sugenotype-D2 were predominant. HBV/D2 isolates from patients with low CD4 count manifested significantly lower non-synonymous substitutions (p<0.0001) and Shannon entropy (p=0.0006) in their surface and polymerase gene in comparison to those from moderately increased CD4 count. ART-induced immune-reconstitution therefore might raise non-synonymous immune/therapy escape substitutions among these HBV/D2 isolates. Decreased genetic diversity and increased viral load in the HBV/D2 isolates might facilitate the maintenance of their wild type characteristics in the low CD4 count, leading to its increased prevalence in this group. Interestingly, genetic diversity in HBV/A1, the next common subgenotype, was modified in the opposite manner. Together our results underscore the need for proper HBV molecular monitoring in HIV co-infection.
authors.affiliation: ICMR Virus Unit, Kolkata, ID & BG Hospital Campus, GB 4, Kolkata 700010, India.
authors.forename: Ananya
authors.initials: A
authors.lastname: Pal
authors.forename: Rajesh
authors.initials: R
authors.lastname: Panigrahi
authors.forename: Avik
authors.initials: A
authors.lastname: Biswas
authors.forename: Sibnarayan
authors.initials: S
authors.lastname: Datta
authors.forename: Neelakshi
authors.initials: N
authors.lastname: Sarkar
authors.forename: Subhashish Kamal
authors.initials: SK
authors.lastname: Guha
authors.forename: Bibhuti
authors.initials: B
authors.lastname: Saha
authors.forename: Arup
authors.initials: A
authors.lastname: Banerjee
authors.forename: Sekhar
authors.initials: S
authors.lastname: Chakrabarti
authors.forename: Runu
authors.initials: R
authors.lastname: Chakravarty
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D019380:Anti-HIV Agents / Q000627:therapeutic use; D018791:CD4 Lymphocyte Count; D060085:Coinfection; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006678:HIV / Q000235:genetics*; D015658:HIV Infections / Q000150:complications / Q000276:immunology*; D006509:Hepatitis B / Q000150:complications / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000276:immunology*; D006515:Hepatitis B virus / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007165:Immunosuppression Therapy; D019259:Lamivudine / Q000627:therapeutic use; D008297:Male; D017422:Sequence Analysis, DNA; D019562:Viral Load
chemical_list: D019380:Anti-HIV Agents; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D019259:Lamivudine