title: A model for the design and construction of a resource for the validation of prognostic prostate cancer biomarkers: the Canary Prostate Cancer Tissue Microarray.
abstract: Tissue microarrays (TMAs) provide unique resources for rapid evaluation and validation of tissue biomarkers. The Canary Foundation Retrospective Prostate Tissue Microarray Resource used a rigorous statistical design, quota sampling, a variation of the case-cohort study, to select patients for inclusion in a multicenter, retrospective prostate cancer TMA cohort. The study is designed to definitively validate tissue biomarkers of prostate cancer recurrence after radical prostatectomy. Tissue samples from over 1000 participants treated for prostate cancer with radical prostatectomy between 1995 and 2004 were selected at 6 participating institutions in the United States and Canada. This design captured the heterogeneity of screening and clinical practices in the contemporary North American population. Standardized clinical data were collected in a centralized database. The project has been informative in several respects. The scale and complexity of assembling TMAs with over 200 cases at each of 6 sites involved unanticipated levels of effort and time. Our statistical design promises to provide a model for outcome-based studies where tissue localization methods are applied to high-density TMAs.
authors.affiliation: Canary Foundation, Palo Alto, CA 94304, USA. sarah@canaryfoundation.org
authors.forename: Sarah
authors.initials: S
authors.lastname: Hawley
authors.forename: Ladan
authors.initials: L
authors.lastname: Fazli
authors.forename: Jesse K
authors.initials: JK
authors.lastname: McKenney
authors.forename: Jeff
authors.initials: J
authors.lastname: Simko
authors.forename: Dean
authors.initials: D
authors.lastname: Troyer
authors.forename: Marlo
authors.initials: M
authors.lastname: Nicolas
authors.forename: Lisa F
authors.initials: LF
authors.lastname: Newcomb
authors.forename: Janet E
authors.initials: JE
authors.lastname: Cowan
authors.forename: Luis
authors.initials: L
authors.lastname: Crouch
authors.forename: Michelle
authors.initials: M
authors.lastname: Ferrari
authors.forename: Javier
authors.initials: J
authors.lastname: Hernandez
authors.forename: Antonio
authors.initials: A
authors.lastname: Hurtado-Coll
authors.forename: Kyle
authors.initials: K
authors.lastname: Kuchinsky
authors.forename: Janet
authors.initials: J
authors.lastname: Liew
authors.forename: Rosario
authors.initials: R
authors.lastname: Mendez-Meza
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Smith
authors.forename: Imelda
authors.initials: I
authors.lastname: Tenggara
authors.forename: Xiaotun
authors.initials: X
authors.lastname: Zhang
authors.forename: Peter R
authors.initials: PR
authors.lastname: Carroll
authors.forename: June M
authors.initials: JM
authors.lastname: Chan
authors.forename: Martin
authors.initials: M
authors.lastname: Gleave
authors.forename: Raymond
authors.initials: R
authors.lastname: Lance
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Lin
authors.forename: Peter S
authors.initials: PS
authors.lastname: Nelson
authors.forename: Ian M
authors.initials: IM
authors.lastname: Thompson
authors.forename: Ziding
authors.initials: Z
authors.lastname: Feng
authors.forename: Lawrence D
authors.initials: LD
authors.lastname: True
authors.forename: James D
authors.initials: JD
authors.lastname: Brooks
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D016208:Databases, Factual / Q000592:standards; D006801:Humans; D008297:Male; D010338:Pathology, Clinical / Q000379:methods / Q000592:standards; D011379:Prognosis; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D015203:Reproducibility of Results; D046888:Tissue Array Analysis / Q000379:methods* / Q000592:standards*
chemical_list: D014408:Biomarkers, Tumor