title: [Chemical constituents from Hypericum perforatum].
abstract: OBJECTIVE
  To study the chemical constituents from Hypericum perforatum.
  
  
  METHODS
  Compouds were isolated by chromatographic techniques. Their structures were identified by spectral methods. The inhibitory activity of recombinant human PTP1B was evaluated.
  
  
  RESULTS
  Nine compounds were elucidated as D-Mannitol (1), 1,2-benzenedicarboxylic acid bis(1-methylpropyl) ester (2), (7E, 6R,9S)-9-hydroxy-4,7-megastigmadien-3-one (3), (6S,9R)-roseoside (4) , 2,6-dimethoxy-4-hydroquinone-l-O-beta-D-glucopyranoside (5), 2,6-di-methoxy-4-hydroxybenzyl alcohol 1 -O-beta-D-glucopyranoside (6), syringate 4-O-beta-glucopyranoside (7), hypericin (8), skyrin-6-0-beta-D-glucopyranoside (9) , (R)-2,3-dihydroxypropyl 3,4-dihydroxy-benzoate (10). At a concentration of 2 micromol x L(-1), compound 8 inhibited recombinant human PTP1B with inhibitory rate of 96.4% and IC50 of 2.5 micromol x L(-1).
  
  
  CONCLUSIONS
  compound 10 was new, compounds 2-4, 7 were obtained from Hypericum for the first time and compounds 5-6 was isolated from this plant for the first time. Compound 8 showed remarkable PTP1B inhibitory activity.
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.forename: Jianbo
authors.initials: J
authors.lastname: Yang
authors.forename: Tengfei
authors.initials: T
authors.lastname: Ji
authors.forename: Aiguo
authors.initials: A
authors.lastname: Wang
authors.forename: Yalun
authors.initials: Y
authors.lastname: Su
mesh_terms: D000880:Anthraquinones / Q000737:chemistry / Q000302:isolation & purification; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification; D020902:Hypericum / Q000737:chemistry*; D015394:Molecular Structure; D045792:Norisoprenoids / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000880:Anthraquinones; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D045792:Norisoprenoids; C438027:roseoside; C009274:skyrin