title: [Research on TLC identification and anti-coagulant activity quantitatively methods of Scolopendra subspinipes mutilans].
abstract: OBJECTIVE
  To improve the quality standard of Scolopendra subspinipes mutilans by researching the methods of the TLC identification and anti-coagulant activity quantitatively.
  
  
  METHODS
  Identified the free arginine (Arg) and serine (Ser) in scolopendra by TLC, screened the samples preparation process and developed solvent systems; Determined the anti-coagulant activity by method of titration with thrombin and screened the pretreatment methods.
  
  
  RESULTS
  When medicinal materials was extracted by formic acid and 95% ethanol (1:1) with ultrasonic method and developed by n-butanol-acetic acid-water (12:5:4), the spots of Arg and Ser were well separated. Ultrasonic method was suitable for preparation of the anti-coagulant components in Scolopendra subspinipes mutilans and their anti-coagulant activity was determined by method of titration with thrombin could get a well reproducibility, the anti-thrombin activity of testing sample was (14.00 +/- 1.53) U/g and those of three different batch were (13.00 +/- 0.58) U/g, (17.00 +/- 1.15) U/g, (15.67 +/- 1.53) U/g respectively.
  
  
  CONCLUSIONS
  The methods of TLC identification and anti-coagulant activity quantitatively could be used as a basis for improving the quality standard of Scolopendra subspinipes mutilans.
authors.affiliation: Key Laboratory of Chinese Materia Medica Preparation of Guangdong Province, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China. litaocatherine@163.com
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Xiao-mei
authors.initials: XM
authors.lastname: Tan
authors.forename: Qun
authors.initials: Q
authors.lastname: Long
authors.forename: Fei-long
authors.initials: FL
authors.lastname: Chen
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001120:Arginine / Q000032:analysis; D001181:Arthropods / Q000737:chemistry; D002855:Chromatography, Thin Layer / Q000379:methods; D008419:Materia Medica / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D011786:Quality Control; D015203:Reproducibility of Results; D012694:Serine / Q000032:analysis; D013678:Technology, Pharmaceutical / Q000379:methods; D013917:Thrombin / Q000032:analysis* / Q000037:antagonists & inhibitors; D017186:Titrimetry / Q000379:methods
chemical_list: D000925:Anticoagulants; D008419:Materia Medica; D012694:Serine; D001120:Arginine; D013917:Thrombin