title: [Detection of Rickettsia in ectoparasites of wild and domestic mammals from the Cerro Chucanti private reserve and from neighboring towns, Panamá, 2007-2010].
abstract: BACKGROUND
  Ectoparasites are the main vectors of rickettsiosis. In Panama, however, limited data are available concerning the arthropod species that serve as vectors or reservoirs.
  
  
  OBJECTIVE
  Data are presented concerning the presence of Rickettsia in ectoparasites of wildlife and domestic animals in the Cerro Chucantí private nature reserve and in neighboring villages.
  
  
  METHODS
  Nine humans, 95 domestic mammals and 48 wild mammals were examined. Twenty-one species of ectoparasites were obtained, including fleas, lice, ticks and mites. These were preserved in 95% ethanol. Later, the DNA was extracted from the ticks and fleas and analyzed by molecular techniques to detect presence of Rickettsia.
  
  
  RESULTS
  Of a total of 425 PCR reactions, 270 were positive for Rickettsia and 155 negative. Among the positive samples, 86 PCR amplified for the gltA gene (55% of positives) and 41 of these also amplified the ompA gene. DNA of Rickettsia amblyommii was found in horses ticks (Amblyomma cajennense, Dermacentor nitens), dogs ticks (Rhipicephalus sanguineus) and free living nymphs in the forest. Additionally, DNA of R. felis was found in fleas from dogs Ctenocephalides felis.
  
  
  CONCLUSIONS
  The presence of R. amblyommii and R. felis was detected in ticks and fleas of domestic animals in villages near Cerro Chucanti; however no Rickettsia DNA was found in ectoparasites of non-domestic wildlife.
authors.affiliation: Departamento de Entomología Médica, Instituto Conmemorativo Gorgas de Estudios de la Salud, Panamá, Panamá. bermudezsec@gmail.com
authors.forename: Sergio
authors.initials: S
authors.lastname: Bermúdez
authors.forename: Roberto
authors.initials: R
authors.lastname: Miranda
authors.forename: Yamitze
authors.initials: Y
authors.lastname: Zaldívar
authors.forename: Publio
authors.initials: P
authors.lastname: González
authors.forename: Guido
authors.initials: G
authors.lastname: Berguido
authors.forename: Diomedes
authors.initials: D
authors.lastname: Trejos
authors.forename: Juan M
authors.initials: JM
authors.lastname: Pascale
authors.forename: Marcelo
authors.initials: M
authors.lastname: Labruna
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology*; D000835:Animals, Wild / Q000382:microbiology*; D001179:Arthropod Vectors / Q000382:microbiology*; D002371:Cat Diseases / Q000469:parasitology; D002415:Cats; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D004269:DNA, Bacterial / Q000032:analysis; D004197:Disease Reservoirs / Q000382:microbiology*; D004283:Dog Diseases / Q000469:parasitology; D004285:Dogs; D004478:Ectoparasitic Infestations / Q000382:microbiology / Q000469:parasitology* / Q000662:veterinary*; D005260:Female; D005798:Genes, Bacterial; D006734:Horse Diseases / Q000469:parasitology; D006736:Horses; D006801:Humans; D007814:Larva; D008297:Male; D008322:Mammals / Q000382:microbiology*; D008925:Mites / Q000382:microbiology; D010176:Panama; D008003:Phthiraptera / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012281:Rickettsia / Q000302:isolation & purification*; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology; D005423:Siphonaptera / Q000382:microbiology; D013045:Species Specificity; D013987:Ticks / Q000254:growth & development / Q000382:microbiology; D014197:Trees
chemical_list: D004269:DNA, Bacterial