title: Decursin inhibits UVB-induced MMP expression in human dermal fibroblasts             via regulation of nuclear factor-κB.
abstract: Decursin, a coumarin compound, was originally isolated from the roots of Angelica gigas almost four decades ago, and it was found to exhibit cytotoxicity against various types of human cancer cells and anti-amnesic activity in vivo through the inhibition of AChE activity. However, the anti-skin photoaging effects of decursin have not been reported to date. In the present study, we investigated the inhibitory effects of decursin on the expression of matrix metalloproteinase (MMP)-1 and MMP-3 in human dermal fibroblast (HDF) cells. Western blot analysis and real-time PCR revealed that decursin inhibited the ultraviolet (UV)B-induced expression of MMP-1 and MMP-3 in a dose-dependent manner. Decursin significantly blocked the UVB-induced activation of nuclear factor-κB (NF-κB). However, decursin showed no effect on MAPK or AP-1 activity. In this study, decursin prevented the UVB-induced expression of MMPs via the inhibition of NF-κB activation. In conclusion, decursin may be a potential agent for the prevention and treatment of skin photoaging.
authors.affiliation: Department of Biochemistry, Institute of Medical Science, Chonbuk                     National University Medical School, Jeonju 560-182, Republic of Korea.
authors.forename: Bo-Mi
authors.initials: BM
authors.lastname: Hwang
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Noh
authors.forename: Jong-Suk
authors.initials: JS
authors.lastname: Kim
authors.forename: Jeong-Mi
authors.initials: JM
authors.lastname: Kim
authors.forename: Jin-Ki
authors.initials: JK
authors.lastname: Hwang
authors.forename: Hye-Kyung
authors.initials: HK
authors.lastname: Kim
authors.forename: Jae-Seon
authors.initials: JS
authors.lastname: Kang
authors.forename: Do-Sung
authors.initials: DS
authors.lastname: Kim
authors.forename: Han-Jung
authors.initials: HJ
authors.lastname: Chae
authors.forename: Yong-Ouk
authors.initials: YO
authors.lastname: You
authors.forename: Kang-Beom
authors.initials: KB
authors.lastname: Kwon
authors.forename: Young-Rae
authors.initials: YR
authors.lastname: Lee
mesh_terms: D029969:Angelica / Q000737:chemistry; D001578:Benzopyrans / Q000302:isolation & purification / Q000494:pharmacology*; D002087:Butyrates / Q000302:isolation & purification / Q000494:pharmacology*; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D020405:Dermis / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000235:genetics* / Q000378:metabolism; D019278:Matrix Metalloproteinase 3 / Q000235:genetics* / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D001578:Benzopyrans; D002087:Butyrates; D016328:NF-kappa B; D018808:Transcription Factor AP-1; D004247:DNA; C101278:decursin; D019278:Matrix Metalloproteinase 3; D020781:Matrix Metalloproteinase 1