title: Sonoclot evaluation of whole blood coagulation in healthy adult dogs.
abstract: OBJECTIVE
  To establish a standard protocol for analysis of canine whole blood and generate reference intervals for healthy dogs using the Sonoclot analyzer, and to compare Sonoclot values to standard and viscoelastic coagulation tests.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  Veterinary University research facility and teaching hospital.
  
  
  METHODS
  Twelve healthy random source dogs and 52 healthy dogs from the general veterinary school population.
  
  
  METHODS
  Blood sampling for viscoelastic coagulation testing.
  
  
  RESULTS
  Blood was collected from 12 healthy adult dogs by jugular venipuncture. After a rest period at room temperature of 30, 60, or 120 minutes, 340 μL of citrated blood was added to 20 μL of 0.2 M CaCl(2)  in 1 of 2 cuvette types warmed to 37° C. Cuvettes contained a magnetic stir-bar with glass beads (gbACT+) or only a magnetic stir-bar (nonACT). Reference interval samples were collected from 52 healthy adult dogs and analyzed in duplicate. The ACT, CR, and PF were not affected by duration of rest period for either cuvette type. ACT variability was decreased when using gbACT+ cuvettes (P < 0.05). In normal dogs reference intervals (mean ± 2 SD) using gbACT+ cuvettes were: ACT 56.0-154.0 seconds, CR 14.85-46.0, and PF 2.1-4.05. ACT correlated to TEG R-time, K-time, and angle, while CR correlated with all TEG parameters. Fibrinogen correlated with ACT, CR, and PF. Sonoclot did not correlate with other common coagulation tests.
  
  
  CONCLUSIONS
  Sonoclot provides viscoelastic evaluation of canine whole blood coagulation and correlated to several TEG parameters and fibrinogen. A standard protocol and reference intervals were established.
authors.affiliation: Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
authors.forename: Danielle M
authors.initials: DM
authors.lastname: Babski
authors.forename: Benjamin M
authors.initials: BM
authors.lastname: Brainard
authors.forename: Paula M
authors.initials: PM
authors.lastname: Krimer
authors.forename: Alan G
authors.initials: AG
authors.lastname: Ralph
authors.forename: Jennifer R
authors.initials: JR
authors.lastname: Pittman
authors.forename: Amie
authors.initials: A
authors.lastname: Koenig
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000502:physiology*; D001780:Blood Coagulation Tests / Q000379:methods / Q000662:veterinary*; D004285:Dogs / Q000097:blood*; D012016:Reference Values; D014914:Whole Blood Coagulation Time / Q000379:methods / Q000662:veterinary*