title: Perchlorate reduction by an isolated Serratia marcescens strain under high salt and extreme pH.
abstract: An isolated Serratia marcescens strain exhibited growth-coupled perchlorate (ClO4 -) reduction under anoxic conditions. Perchlorate was reduced completely with stoichiometric chloride buildup and equimolar acetate consumption. Polymerase chain reaction confirmed the presence of pcrA and cld genes coding for key enzymes involved in the ClO4 - degradation pathway. The isolate degraded ClO4 - under high salt (up to 15% NaCl) and a wide range of pH (4.0-9.0), as well as simultaneously reduced nitrate and ClO4 -.
authors.affiliation: Environmental Technology, CSIR-National Institute for Interdisciplinary Science & Technology, Thiruvananthapuram, India.
authors.forename: Anupama
authors.initials: A
authors.lastname: Vijaya Nadaraja
authors.forename: Prajeesh
authors.initials: P
authors.lastname: Gangadharan Puthiya Veetil
authors.forename: Krishnakumar
authors.initials: K
authors.lastname: Bhaskaran
mesh_terms: D000085:Acetates / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics; D004265:DNA Helicases / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010472:Perchlorates / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012492:Salts / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012706:Serratia marcescens / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000085:Acetates; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010472:Perchlorates; D012492:Salts; C084217:pcrA protein, Bacteria; C075969:rol protein, Bacteria; D004265:DNA Helicases; C494474:perchlorate