title: In vitro transfection mediated by dendrigraft poly(L-lysines): the effect of structure and molecule size.
abstract: Dendritic poly(L-lysines) (DGL) constitute promising nanomaterials applicable as a nonviral gene-delivery vector. In this study, we evaluate the transfection abilities of four DGL generations with special emphasis on the systematic description of the relationship of how generation (i.e., molecule size) affects the transfection efficacy. Using Hep2 cells, we demonstrated that the capability of unmodified DGL to deliver plasmid is of a magnitude lower than that of jetPEI. On the other hand, employing the Hep2 cell line stably transduced with eGFP, we observed that DGL G5 delivers the siRNA oligonucleotide with the same efficiency as Lipofectamine 2000. In further experiments, it was shown that DGL affords excellent ability to bind DNA, protect it against DNase I attack, and internalize it into cells.
authors.affiliation: Generi Biotech Ltd., Machkova 587, 500 11 Hradec Kralove, Czech Republic.
authors.forename: Jakub
authors.initials: J
authors.lastname: Hofman
authors.forename: Martin
authors.initials: M
authors.lastname: Buncek
authors.forename: Radovan
authors.initials: R
authors.lastname: Haluza
authors.forename: Ludvik
authors.initials: L
authors.lastname: Streinz
authors.forename: Miroslav
authors.initials: M
authors.lastname: Ledvina
authors.forename: Petr
authors.initials: P
authors.lastname: Cigler
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003850:Deoxyribonuclease I / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D008055:Lipids; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000378:metabolism / Q000493:pharmacokinetics; D010957:Plasmids / Q000493:pharmacokinetics; D011107:Polylysine / Q000737:chemistry* / Q000493:pharmacokinetics*; D034741:RNA, Small Interfering; D013329:Structure-Activity Relationship; D014162:Transfection / Q000379:methods*
chemical_list: D008055:Lipids; C086724:Lipofectamine; D009841:Oligonucleotides; D034741:RNA, Small Interfering; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D011107:Polylysine; D003850:Deoxyribonuclease I