title: Expression of mRNAs of urocortin and corticotropin-releasing factor receptors in malignant glioma cell lines.
abstract: BACKGROUND
  Urocortin and corticotropin-releasing factors (CRFs) and their receptors are expressed in many organs, including the central nervous system. In this study, the expression of mRNAs of urocortin 1, 2, 3, and CRF and CRF receptors 1 and 2 in malignant glioma, was examined.
  
  
  METHODS
  The RNAs of human and rat glioma cell lines were isolated. Transcripts in these cells were analyzed using cDNA. In addition, the effects of proliferative and cytotoxic stimulation by serum supplementation, ionizing radiation, and the antineoplastic agent temozolomide were investigated.
  
  
  RESULTS
  Human and rat cells transcribed urocortin. CRF receptors were detected in human glioma cells. When human KNS42 cells were exposed to stimulation, transcription was altered according to the specific condition.
  
  
  CONCLUSIONS
  Expression of mRNAs of urocortin and CRF receptors was confirmed in human glioma cell lines. Although the quantities of transcripts varied with the proliferative and cytotoxic stimulation, the overall transcription pattern was not influenced by these stimuli.
authors.affiliation: Department of Molecular Cell Biology, Jikei University School of Medicine, 3-25-8 Nishishinbashi, Minato-ku, Tokyo, Japan 105-8461.
authors.forename: Minori
authors.initials: M
authors.lastname: Kamada
authors.forename: Keiichi
authors.initials: K
authors.lastname: Ikeda
authors.forename: Kouki
authors.initials: K
authors.lastname: Fujioka
authors.forename: Noibutake
authors.initials: N
authors.lastname: Akiyama
authors.forename: Kouhei
authors.initials: K
authors.lastname: Akiyoshi
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Inoue
authors.forename: Sanshiro
authors.initials: S
authors.lastname: Hanada
authors.forename: Kenji
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Tojo
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Manome
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D005910:Glioma / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D018019:Receptors, Corticotropin-Releasing Hormone / Q000096:biosynthesis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D054832:Urocortins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D020033:Protein Isoforms; D012333:RNA, Messenger; D018019:Receptors, Corticotropin-Releasing Hormone; D054832:Urocortins