title: P53-induced microRNA-107 inhibits proliferation of glioma cells and down-regulates the expression of CDK6 and Notch-2.
abstract: MicroRNAs (miRNAs) are small noncoding RNAs that function as tumor suppressors or oncogenes. MicroRNA-107 (miR-107), a transcriptional target of p53, is deregulated in many cancer cell lines. Here, we showed that miR-107 is down-regulated in glioma tissues and cell lines, in particular, p53-mutated U251 and A172. Transfection of wild-type p53 into these cells stimulated miR-107 expression. To investigate the role of miR-107 in tumorigenesis, we constructed a lentiviral vector overexpressing miR-107. Notably, miR-107 inhibited proliferation and arrested the cell cycle at the G0-G1 phase in glioma cells. Transduction of Lenti-GFP-miR-107 into glioma cells inhibited CDK6 and Notch-2 protein expression. Our findings collectively demonstrate that p53-induced miR-107 suppresses brain tumor cell growth and down-regulates CDK6 and Notch-2 expression, supporting its tumor suppressor role and utility as a target for glioma therapy.
authors.affiliation: Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Run
authors.initials: R
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Kun
authors.initials: K
authors.lastname: Qin
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Fa
authors.forename: Yi-Jing
authors.initials: YJ
authors.lastname: Liu
authors.forename: Yi-Quan
authors.initials: YQ
authors.lastname: Ke
authors.forename: Xiao-Dan
authors.initials: XD
authors.lastname: Jiang
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D051361:Cyclin-Dependent Kinase 6 / Q000096:biosynthesis*; D015536:Down-Regulation; D005910:Glioma; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D051883:Receptor, Notch2 / Q000096:biosynthesis*; D014161:Transduction, Genetic; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*
chemical_list: C549045:MIRN107 microRNA, human; D035683:MicroRNAs; C497764:NOTCH2 protein, human; D051883:Receptor, Notch2; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C495911:CDK6 protein, human; D051361:Cyclin-Dependent Kinase 6