title: A Novel Herbal Medicine KIOM-MA Exerts an Anti-Inflammatory Effect in LPS-Stimulated RAW 264.7 Macrophage Cells.
abstract: KIOM-MA was recently reported as a novel herbal medicine effective for atopic dermatitis and asthma. In this study, we have demonstrated the inhibitory effect of KIOM-MA on proinflammatory mediator produced in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. KIOM-MA significantly inhibited the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) as well as nitric oxide (NO) and prostaglandin E(2) (PGE(2)). Consistent with the inhibitory effect on PGE(2), KIOM-MA suppresses the LPS-induced migration of macrophages and gelatinase activity and the expression of matrix metalloprotease-9 (MMP-9) in a dose-dependent manner. Additionally, KIOM-MA showed a strong suppressive effect on the inflammatory cytokines production such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). We also found that KIOM-MA inhibits the activation of nuclear factor-κB (NF-κB) and represses the activity of extracellular signal-regulated kinase (ERK), p38, and c-Jun NH(2)-terminal kinase (JNK) mitogen-activated protein kinases (MAPKs). Taken together, we elucidated the mechanism of anti-inflammatory effect of KIOM-MA using RAW 264.7 cells stimulated by LPS.
authors.affiliation: Korean Medicine (KM)-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine, 461-24 Jeonmin-Dong, Yuseong, Daejeon 305-811, Republic of Korea.
authors.forename: You-Chang
authors.initials: YC
authors.lastname: Oh
authors.forename: Won-Kyung
authors.initials: WK
authors.lastname: Cho
authors.forename: Yun Hee
authors.initials: YH
authors.lastname: Jeong
authors.forename: Ga Young
authors.initials: GY
authors.lastname: Im
authors.forename: Aeyung
authors.initials: A
authors.lastname: Kim
authors.forename: Youn-Hwan
authors.initials: YH
authors.lastname: Hwang
authors.forename: Taesoo
authors.initials: T
authors.lastname: Kim
authors.forename: Kwang Hoon
authors.initials: KH
authors.lastname: Song
authors.forename: Jin Yeul
authors.initials: JY
authors.lastname: Ma