title: UV damage endonuclease employs a novel dual-dinucleotide flipping mechanism to recognize different DNA lesions.
abstract: Repairing damaged DNA is essential for an organism's survival. UV damage endonuclease (UVDE) is a DNA-repair enzyme that can recognize and incise different types of damaged DNA. We present the structure of Sulfolobus acidocaldarius UVDE on its own and in a pre-catalytic complex with UV-damaged DNA containing a 6-4 photoproduct showing a novel 'dual dinucleotide flip' mechanism for recognition of damaged dipyrimidines: the two purines opposite to the damaged pyrimidine bases are flipped into a dipurine-specific pocket, while the damaged bases are also flipped into another cleft.
authors.affiliation: Department of Biophysical Structural Chemistry, Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands.
authors.forename: Elisabeth M
authors.initials: EM
authors.lastname: Meulenbroek
authors.forename: Caroline
authors.initials: C
authors.lastname: Peron Cane
authors.forename: Isabelle
authors.initials: I
authors.lastname: Jala
authors.forename: Shigenori
authors.initials: S
authors.lastname: Iwai
authors.forename: Geri F
authors.initials: GF
authors.lastname: Moolenaar
authors.forename: Nora
authors.initials: N
authors.lastname: Goosen
authors.forename: Navraj S
authors.initials: NS
authors.lastname: Pannu
mesh_terms: D000595:Amino Acid Sequence; D004247:DNA / Q000737:chemistry; D004249:DNA Damage; D045643:DNA Repair Enzymes / Q000737:chemistry* / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000737:chemistry* / Q000378:metabolism; D008670:Metals / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011740:Pyrimidine Dimers / Q000378:metabolism; D016415:Sequence Alignment; D016945:Sulfolobus acidocaldarius / Q000201:enzymology
chemical_list: D008670:Metals; D011740:Pyrimidine Dimers; C058406:pyrimidine-pyrimidone dimer; D004247:DNA; D004706:Endodeoxyribonucleases; D045643:DNA Repair Enzymes