title: Expression of miR-150 and miR-3940-5p is reduced in non-small cell lung             carcinoma and correlates with clinicopathological features.
abstract: The present study investigated the expression of miR-150 and miR-3940-5p             in non-small cell lung carcinoma (NSCLC) and its relationship with clinicopathologic             features. Samples included tumor, tumor-adjacent and normal lung parenchyma tissues             from 90 NSCLC patients and 17 cases of embryonic lung cDNA. The expression levels             of miR-150, miR-18b-5p, miR-643 and miR-3940-5p were detected by real‑time PCR;             p53, EGFR, Kras and Ki-67 expression in tumor tissues was determined by immunohistochemistry.             p53 mRNA expression levels in NSCLC were examined by SYBR-Green real-time PCR.             The relationship between the four miRNAs and clinicopathologic features of 90             cases was analyzed. The expression of miR-150 and miR-3940-5p was significantly             downregulated in tumor tissues and embryonic lung tissues compared to normal lung             tissues. The expression of miR-150 and miR-3940-5p in tumor tissues was also lower             than that in the matched tumor-adjacent tissues. miR-150 was downregulated preferentially             in subgroups of patients with a tumor diameter more than or equal to 3 cm, in             smokers and in stage III and IV tumors. Specifically, miR-150 and miR-3940-5p             expression was decreased in nuclear cell proliferation antigen Ki-67-positive             NSCLC cases. miR-150 and miR-3940-5p were found to be significantly downregulated             in p53 IHC-positive NSCLC cases and were negatively correlated with p53 mRNA.             Reduced miR-150 and miR-3940-5p expression in tumor tissues and embryonic lung             tissues suggests that these miRs may be involved in the tumorigenesis or de-differentiation             of NSCLC. Due to this associaton with the Ki-67 proliferation index in NSCLC,             downregulation of miR-150 and miR-3940-5p may contribute to tumor growth and proliferation.             miR-150 and miR-3940-5p may affect p53 expression through a direct or indirect             pathway.
authors.affiliation: Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai 200433, PR China.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Sun
authors.forename: Bo
authors.initials: B
authors.lastname: Su
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Huikang
authors.initials: H
authors.lastname: Xie
authors.forename: Hui
authors.initials: H
authors.lastname: Zheng
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Xu
authors.forename: Qiaoling
authors.initials: Q
authors.lastname: Du
authors.forename: Huan
authors.initials: H
authors.lastname: Zeng
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhou
authors.forename: Chang
authors.initials: C
authors.lastname: Chen
authors.forename: Wen
authors.initials: W
authors.lastname: Gao
mesh_terms: D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000556:secondary*; D016009:Chi-Square Distribution; D015536:Down-Regulation; D066246:ErbB Receptors / Q000378:metabolism; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D008168:Lung / Q000196:embryology / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D016283:Proto-Oncogene Proteins p21(ras); D012333:RNA, Messenger / Q000378:metabolism; D018709:Statistics, Nonparametric; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D018631:ras Proteins / Q000378:metabolism
chemical_list: D066246:ErbB Receptors; D019394:Ki-67 Antigen; D035683:MicroRNAs; D011518:Proto-Oncogene Proteins; D016283:Proto-Oncogene Proteins p21(ras); D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D018631:ras Proteins; C117307:KRAS protein, human; C533097:MIR150, human; C581632:MIRN18 microRNA, human; C581631:MIRN3940 microRNA, human; C581633:MIRN643 microRNA, human