title: Mammalian prions: tolerance to sequence changes-how far?
abstract: Upon prion infection, abnormal prion protein (PrP (Sc) ) self-perpetuate by conformational conversion of α-helix-rich PrP (C) into β sheet enriched form, leading to formation and deposition of PrP (Sc) aggregates in affected brains. However the process remains poorly understood at the molecular level and the regions of PrP critical for conversion are still debated. Minimal amino acid substitutions can impair prion replication at many places in PrP. Conversely, we recently showed that bona fide prions could be generated after introduction of eight and up to 16 additional amino acids in the H2-H3 inter-helix loop of PrP. Prion replication also accommodated the insertions of an octapeptide at different places in the last turns of H2. This reverse genetic approach reveals an unexpected tolerance of prions to substantial sequence changes in the protease-resistant part which is associated with infectivity. It also demonstrates that conversion does not require the presence of a specific sequence in the middle of the H2-H3 area. We discuss the implications of our findings according to different structural models proposed for PrP (Sc) and questioned the postulated existence of an N- or C-terminal prion domain in the protease-resistant region.
authors.affiliation: INRA, UR892 Virologie Immunologie Moléculaires, Jouy-en-Josas, France.
authors.forename: Muhammad Khalid
authors.initials: MK
authors.lastname: Salamat
authors.forename: Carola
authors.initials: C
authors.lastname: Munoz-Montesino
authors.forename: Mohammed
authors.initials: M
authors.lastname: Moudjou
authors.forename: Human
authors.initials: H
authors.lastname: Rezaei
authors.forename: Hubert
authors.initials: H
authors.lastname: Laude
authors.forename: Vincent
authors.initials: V
authors.lastname: Béringue
authors.forename: Michel
authors.initials: M
authors.lastname: Dron
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D006801:Humans; D008322:Mammals; D008969:Molecular Sequence Data; D009154:Mutation; D017096:Prion Diseases / Q000378:metabolism; D011328:Prions / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D011487:Protein Conformation; D015202:Protein Engineering; D013329:Structure-Activity Relationship
chemical_list: D011328:Prions