title: Centrosomal localization of RhoGDIβ and its relevance to mitotic processes             in cancer cells.
abstract: Rho GDP-dissociation inhibitors (RhoGDIs) are regulators of Rho family GTPases.             RhoGDIβ has been implicated in cancer progression, but its precise role remains             unclear. We determined the subcellular localization of RhoGDIβ and examined the             effects of its overexpression and RNAi knockdown in cancer cells. Immunofluorescence             staining showed that RhoGDIβ localized to centrosomes in human cancer cells. In             HeLa cells, exogenous GFP-tagged RhoGDIβ localized to centrosomes and its overexpression             caused prolonged mitosis and aberrant cytokinesis in which the cell shape was             distorted. RNAi knockdown of RhoGDIβ led to increased incidence of monopolar spindle             mitosis resulting in polyploid cells. These results suggest that RhoGDIβ has mitotic             functions, including regulation of cytokinesis and bipolar spindle formation.             The dysregulated expression of RhoGDIβ may contribute to cancer progression by             disrupting these processes.
authors.affiliation: Division of Tumor Biology, Department of Life Science, Medical                     Research Institute, Kanazawa Medical University, Uchinada, Ishikawa 920-0293,                     Japan.
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Jiang
authors.forename: Masayo
authors.initials: M
authors.lastname: Maeda
authors.forename: Mayumi
authors.initials: M
authors.lastname: Okamoto
authors.forename: Mikiko
authors.initials: M
authors.lastname: Fujii
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Fukutomi
authors.forename: Masato
authors.initials: M
authors.lastname: Hori
authors.forename: Masaaki
authors.initials: M
authors.lastname: Tatsuka
authors.forename: Takahide
authors.initials: T
authors.lastname: Ota
mesh_terms: D018385:Centrosome / Q000648:ultrastructure*; D048749:Cytokinesis / Q000235:genetics; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D034622:RNA Interference; D008941:Spindle Apparatus / Q000235:genetics; D062949:rho Guanine Nucleotide Dissociation Inhibitor beta / Q000737:chemistry / Q000235:genetics*
chemical_list: D062949:rho Guanine Nucleotide Dissociation Inhibitor beta