title: Determination of gymnemagenin in rat plasma using high-performance liquid chromatography-tandem mass spectrometry: application to pharmacokinetics after oral administration of Gymnema sylvestre extract.
abstract: A sensitive and rapid high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method has been developed and validated for the determination of gymnemagenin (GMG), a triterpene sapogenin from Gymnema sylvestre, in rat plasma using withaferin A as the internal standard (IS). Plasma samples were simply extracted using liquid-liquid extraction with tetra-butyl methyl ether. Chromatographic separation was performed on Luna C(18) column using gradient elution of water and methanol (with 0.1% formic acid and 0.3% ammonia) at a flow rate of 0.8 mL/min. GMG and IS were eluted at 4.64 and 4.36 min, ionized in negative and positive mode, respectively, and quantitatively estimated using multiple reaction monitoring (MRM) mode. Two MRM transitions were selected at m/z 505.70 → 455.5 and m/z 471.50 → 281.3 for GMG and IS, respectively. The assay was linear over the concentration range of 5.280-300.920 ng/mL. The mean plasma extraction recoveries for GMG and IS were found to be 80.92 ± 8.70 and 55.63 ± 0.76%, respectively. The method was successfully applied for the determination of pharmacokinetic parameters of GMG after oral administration of G. sylvestre extract.
authors.affiliation: Department of Pharmacognosy, J.S.S. College of Pharmacy, Rocklands, Ootacamund 643 001, Tamilnadu, India. k.bhagyashree@gmail.com
authors.forename: Bhagyashree
authors.initials: B
authors.lastname: Kamble
authors.forename: Ankur
authors.initials: A
authors.lastname: Gupta
authors.forename: Dada
authors.initials: D
authors.lastname: Patil
authors.forename: Laxman
authors.initials: L
authors.lastname: Khatal
authors.forename: Shirish
authors.initials: S
authors.lastname: Janrao
authors.forename: Ismail
authors.initials: I
authors.lastname: Moothedath
authors.forename: Basavan
authors.initials: B
authors.lastname: Duraiswamy
mesh_terms: D000284:Administration, Oral; D000470:Alkaloids / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004347:Drug Interactions; D004355:Drug Stability; D031104:Gymnema sylvestre / Q000737:chemistry*; D016018:Least-Squares Analysis; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000470:Alkaloids; D010936:Plant Extracts; C517988:gymnemagenin