title: Urine proteome of autosomal dominant polycystic kidney disease patients.
abstract: BACKGROUND
  Autosomal dominant polycystic kidney disease (ADPKD) is responsible for 10% of cases of the end stage renal disease. Early diagnosis, especially of potential fast progressors would be of benefit for efficient planning of therapy. Urine excreted proteome has become a promising field of the search for marker patterns of renal diseases including ADPKD. Up to now however, only the low molecular weight fraction of ADPKD proteomic fingerprint was studied. The aim of our study was to characterize the higher molecular weight fraction of urinary proteome of ADPKD population in comparison to healthy controls as a part of a general effort aiming at exhaustive characterization of human urine proteome in health and disease, preceding establishment of clinically useful disease marker panel.
  
  
  RESULTS
  We have analyzed the protein composition of urine retentate (>10 kDa cutoff) from 30 ADPKD patients and an appropriate healthy control group by means of a gel-free relative quantitation of a set of more than 1400 proteins. We have identified an ADPKD-characteristic footprint of 155 proteins significantly up- or downrepresented in the urine of ADPKD patients. We have found changes in proteins of complement system, apolipoproteins, serpins, several growth factors in addition to known collagens and extracellular matrix components. For a subset of these proteins we have confirmed the results using an alternative analytical technique.
  
  
  CONCLUSIONS
  Obtained results provide basis for further characterization of pathomechanism underlying the observed differences and establishing the proteomic prognostic marker panel.
authors.affiliation: Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland. michald@ibb.waw.pl.
authors.forename: Magda
authors.initials: M
authors.lastname: Bakun
authors.forename: Mariusz
authors.initials: M
authors.lastname: Niemczyk
authors.forename: Dominik
authors.initials: D
authors.lastname: Domanski
authors.forename: Radek
authors.initials: R
authors.lastname: Jazwiec
authors.forename: Anna
authors.initials: A
authors.lastname: Perzanowska
authors.forename: Stanislaw
authors.initials: S
authors.lastname: Niemczyk
authors.forename: Michal
authors.initials: M
authors.lastname: Kistowski
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Fabijanska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Borowiec
authors.forename: Leszek
authors.initials: L
authors.lastname: Paczek
authors.forename: Michal
authors.initials: M
authors.lastname: Dadlez