title: [Application of STR genetic marker system in the detection of hemophilia A carriers in Guangxi, China].
abstract: OBJECTIVE
  To establish a fast and simple genetic diagnosis technique based on a reliable, short tandem repeat (STR) genetic marker system for the detection of hemophilia A carriers in Guangxi, China.
  
  
  METHODS
  Fluorescent PCR and capillary electrophoresis were used for allele genotyping at three intragenic/extragenic STR loci (F8Int13, DXS1073, and DXS9901) of FVIII gene in the members of 10 hemophilia A families in Guangxi, so as to evaluate the diagnostic efficiency of the STR genetic marker system for detection of hemophilia A carriers. Then the STR genetic marker system was used to detect hemophilia A carriers among examinees.
  
  
  RESULTS
  In the 10 hemophilia A families, 11 confirmed female carriers had the same allele fragment lengths at the three STR loci (F8Int13, DXS1073, and DXS9901) as the probands. Of the 8 females examined, 5 had allele fragments at the three STR loci (F8Int13, DXS1073, and DXS9901) which were identical to those of the probands, and thus they were diagnosed as hemophilia A carriers.
  
  
  CONCLUSIONS
  Genetic analysis at the three STR loci (F8Int13, DXS1073, and DXS9901) can be used to detect hemophilia A carriers rapidly and provide reliable basis for prenatal diagnosis of hemophilia A.
authors.affiliation: Department of Pediatrics, First Affiliated Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Jun-Li
authors.initials: JL
authors.lastname: Zhou
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Wei
authors.forename: Hua
authors.initials: H
authors.lastname: Wu
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Hu
authors.forename: Wei-Ling
authors.initials: WL
authors.lastname: Liang
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D002681:China; D005260:Female; D006580:Genetic Carrier Screening; D005838:Genotype; D006467:Hemophilia A / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged