title: Salinity-induced changes in S-nitrosylation of pea mitochondrial proteins.
abstract: Together with reactive oxygen species, nitric oxide is an essential part of the signal transduction induced by stress conditions. In this work we study the pattern of S-nitrosylated proteins from mitochondria of pea plants subjected to 150mM NaCl for 5 and 14days. A differential pattern of target proteins was found during plant development and salt stress, with a minor number of S-nitrosylated proteins at 14 days specifically some key enzymes related to respiration and photorespiration. At this time of stress, only ATP synthase β subunit, peroxiredoxin and Hsp90 were S-nitrosylated and no changes in protein levels were observed, although the activity of PrxII F may be reduced by S-nitrosylation. The NADH/NAD(+) ratio was also high at 14days but not the NADPH/NADP(+). An enhancement in NO measured by fluorimetry and confocal microscopy was observed in leaves, being part of the NO localized in mitochondria. An increase in mitochondrial GSNOR activity was produced in response to short and long-term NaCl treatment, where a higher number of nitrated proteins were also observed. The results indicated that posttranslational modifications seem to modulate respiratory and photorespiratory pathways, as well as some antioxidant enzymes, through differential S-nitrosylation/denitrosylation in control conditions and under salt stress.
authors.affiliation: Dpt. Stress Biology and Plant Pathology, CEBAS-CSIC, Murcia, Spain.
authors.forename: Daymi
authors.initials: D
authors.lastname: Camejo
authors.forename: María Del Carmen
authors.initials: Mdel C
authors.lastname: Romero-Puertas
authors.forename: María
authors.initials: M
authors.lastname: Rodríguez-Serrano
authors.forename: Luisa María
authors.initials: LM
authors.lastname: Sandalio
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Lázaro
authors.forename: Ana
authors.initials: A
authors.lastname: Jiménez
authors.forename: Francisca
authors.initials: F
authors.lastname: Sevilla
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D018532:Pisum sativum / Q000254:growth & development / Q000378:metabolism; D054464:Peroxiredoxins / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D054712:Salinity
chemical_list: D024101:Mitochondrial Proteins; D010940:Plant Proteins; D009569:Nitric Oxide; D054464:Peroxiredoxins; D000445:Aldehyde Oxidoreductases; C087006:formaldehyde dehydrogenase, glutathione-independent