title: Bioactivity and hemocompatibility study of amorphous hydrogenated carbon coatings produced by pulsed magnetron discharge.
abstract: Literature contains very few data about the potential biomedical application of amorphous hydrogenated carbon (a-C:H) thin films deposited by reactive pulsed magnetron discharge even so it is one of the most scalable plasma deposition technique. In this article, we show that such a C2H2 pulsed magnetron plasma produces high quality coating with good hemocompatibility and bioactive response: no effect on hemolysis and hemostasis were observed, and proliferation of various cell types such as endothelial, fibroblast, and osteoblast-like cells was not affected when the deposition conditions were varied. Cell growth on a-C:H coatings is proposed to take place by a two-step process: the initial cell contact is affected by the smooth topography of the a-C:H coatings, whereas the polymeric-like structure, together with a moderate hydrophilicity and a high hydrogen content, directs the posterior cell spreading while preserving the hemocompatible behavior.
authors.affiliation: Namur Research Institute for Life Sciences (NARILIS), Research Centre for the Physics of Matter and Radiation (PMR), University of Namur (FUNDP), Namur, Belgium. mclopezs@fundp.ac.be
authors.forename: C
authors.initials: C
authors.lastname: Lopez-Santos
authors.forename: J L
authors.initials: JL
authors.lastname: Colaux
authors.forename: J
authors.initials: J
authors.lastname: Laloy
authors.forename: M
authors.initials: M
authors.lastname: Fransolet
authors.forename: F
authors.initials: F
authors.lastname: Mullier
authors.forename: C
authors.initials: C
authors.lastname: Michiels
authors.forename: J-M
authors.initials: JM
authors.lastname: Dogné
authors.forename: S
authors.initials: S
authors.lastname: Lucas
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects / Q000648:ultrastructure; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006847:Hydrocarbons, Iodinated / Q000737:chemistry; D006865:Hydrogenation / Q000187:drug effects; D016209:Interleukin-8 / Q000378:metabolism; D060526:Magnetic Fields; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D056951:Photoelectron Spectroscopy; D013500:Surface Tension / Q000187:drug effects; D014867:Water / Q000737:chemistry; D017155:Wettability / Q000187:drug effects
chemical_list: D020099:Coated Materials, Biocompatible; D006847:Hydrocarbons, Iodinated; D016209:Interleukin-8; D014867:Water; C027946:methylene iodide