title: Indirect enantioseparation of proteinogenic amino acids using naproxen-based chiral derivatizing reagent and HPLC.
abstract: Diastereomers of 18 proteinogenic amino acids were synthesized under microwave irradiation and by vortexing using (S)-naproxen-benzotriazole as chiral derivatizing reagent. The diastereomers synthesized by two approaches were found to be identical in terms of their characterization and chromatographic data. A linear gradient of triethylammonium phosphate (pH 3.5)-acetonitrile (30-65%, within 35 min) was found to be successful using reversed-phase high-performance liquid chromatography for their separation. Detection was carried out at 231 nm and sharp peaks were obtained. The method was validated for accuracy, precision and limit of detection.
authors.affiliation: Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, 247667, India. rbushfcy@iitr.ernet.in
authors.forename: Ravi
authors.initials: R
authors.lastname: Bhushan
authors.forename: Hariom
authors.initials: H
authors.lastname: Nagar
mesh_terms: D000596:Amino Acids / Q000737:chemistry* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase; D057230:Limit of Detection; D016014:Linear Models; D009288:Naproxen / Q000031:analogs & derivatives / Q000737:chemistry*; D015203:Reproducibility of Results; D013237:Stereoisomerism; D014230:Triazoles / Q000737:chemistry
chemical_list: D000596:Amino Acids; D014230:Triazoles; D009288:Naproxen; C012771:benzotriazole