title: [Cloning and expression of luxS and pfs and in vitro biosynthesis autoinducer 2 of avian pathogenic Escherichia coli from Anhui Province].
abstract: OBJECTIVE
  The LuxS/AI-2 quorum sensing (QS) system shared by Gram-positive and Gram-negative bacteria involves the production of autoinducer-2 (AI-2). In this study, the method of biosynthesis of AI-2 was established using recombinant LuxS and Pfs of avian pathogenic Escherichia coli (APEC), which will be benefit for future study of the role of AI-2 in APEC.
  
  
  METHODS
  We investigated AI-2 production in APEC by vibrio harveyi BB170 (BB170). Furthermore, APEC LuxS and Pfs were expressed, purified and used to investigate the production of AI-2 in vitro. LuxS and Pfs were incubated with S-ribosylhomocysteine (SAH), the reaction was detected for the production of luminescence of BB170.
  
  
  RESULTS
  APEC can produce AI-2 by BB170 bioassay. Purified LuxS and Pfs enzymes incubated with SAH and produced 300 micromol/L AI-2 in the reaction products.
  
  
  CONCLUSIONS
  The results demonstrated that recombinant Pfs and LuxS synthesize AI-2 in vitro from SAH. These findings will be of benefit to future studies of the role of AI-2 in APEC.
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, China. hanxgan@163.com
authors.forename: Xiangan
authors.initials: X
authors.lastname: Han
authors.forename: Hao
authors.initials: H
authors.lastname: Bai
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Wenjing
authors.initials: W
authors.lastname: Chen
authors.forename: Chan
authors.initials: C
authors.lastname: Ding
authors.forename: Qinghai
authors.initials: Q
authors.lastname: Hu
authors.forename: Kezong
authors.initials: K
authors.lastname: Qi
authors.forename: Shengqing
authors.initials: S
authors.lastname: Yu
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000382:microbiology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001715:Bird Diseases / Q000382:microbiology*; D013437:Carbon-Sulfur Lyases / Q000235:genetics* / Q000378:metabolism; D002645:Chickens; D002681:China; D003001:Cloning, Molecular; D004372:Ducks; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D015964:Gene Expression Regulation, Bacterial; D006710:Homocysteine / Q000031:analogs & derivatives / Q000378:metabolism; D006714:Homoserine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D007783:Lactones; D009699:N-Glycosyl Hydrolases / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D007783:Lactones; C091088:N-octanoylhomoserine lactone; C487472:S-ribosyl-L-homocysteine; D006710:Homocysteine; D006714:Homoserine; D009699:N-Glycosyl Hydrolases; C019606:adenosylhomocysteine nucleosidase; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria