title: Study of Astragalus mongholicus polysaccharides on endothelial cells permeability induced by HMGB1.
abstract: Astragalus mongholicus polysaccharide (APS) shows various biological activities. Here, we explored the effect of APS on high mobility group protein 1 (HMGB1) -induced endothelial cell permeability. The results indicated APS pretreatment effectively inhibited HMGB1-induced increased permeability in endothelial cells (ECs). Signal transduction studies showed APS inhibited not only the activation of small guanylate Rho and its downstream effector Rho kinase (ROCK), but also the subsequent phosphorylation of myosin light chain (MLC) in ECs. In conclusion, our investigations suggested that APS inhibited HMGB1-induced increased permeability in ECs, regulated by Rho/ROCK signal pathways.
authors.affiliation: Emergency Department, Chongming Branch, Xin Hua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 202150, China. zyjgyh@hotmail.com
authors.forename: Yun-Jiang
authors.initials: YJ
authors.lastname: Zheng
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Zhi-Fang
authors.initials: ZF
authors.lastname: Song
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.forename: Ru-Yuan
authors.initials: RY
authors.lastname: Zhang
authors.forename: Yao-Qing
authors.initials: YQ
authors.lastname: Tang
mesh_terms: D027883:Astragalus Plant / Q000737:chemistry; D002463:Cell Membrane Permeability / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology; D042783:Endothelial Cells / Q000187:drug effects*; D024243:HMGB1 Protein / Q000037:antagonists & inhibitors / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects; D006801:Humans; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D054460:rho-Associated Kinases / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D024243:HMGB1 Protein; D011134:Polysaccharides; D054460:rho-Associated Kinases