title: Evaluation of the quality of blood components obtained after automated separation of whole blood by a new multiunit processor.
abstract: BACKGROUND
  The Reveos system (Terumo BCT) is a fully automated device able to process four whole blood (WB) units simultaneously into a plasma unit, a red blood cell (RBC) unit, and an interim platelet (PLT) unit (IPU). Multiple IPUs can be pooled to form a transfusable PLT product. The aim of our study was to evaluate the quality of components made with the Reveos system from either fresh (2-8 hr) or overnight-held WB.
  
  
  METHODS
  A prototype of the Reveos system was used to process WB. RBCs were resuspended in SAGM, leukoreduced, and assayed for in vitro quality variables during a 42-day storage period at 2 to 6 °C. Twenty-four-hour in vivo recovery was determined on Day 42. Plasma was assayed for cellular contamination and activation variables. IPUs were pooled with SSP+ additive solution for in vitro quality assessments during a 7-day storage period at room temperature.
  
  
  RESULTS
  Reveos-produced RBCs and plasma units met the predefined requirements. RBC recovery was superior to control units. On Day 42, hemolysis was below 0.8% and in vivo recovery was above 75% for all RBCs. Cellular contamination was lower for Reveos-produced plasma. PLT yield was higher with overnight-stored WB. PLT quality was well maintained during storage with no significant differences between the two groups.
  
  
  CONCLUSIONS
  Blood components prepared with the Reveos from fresh or overnight-held WB meet quality criteria without any relevant difference between the two groups. The Reveos system has the potential to increase efficacy and standardization of blood component preparation.
authors.affiliation: Department of Product and Process Development, Sanquin Blood Bank, Amsterdam, The Netherlands. j.lagerberg@sanquin.nl
authors.forename: Johan W
authors.initials: JW
authors.lastname: Lagerberg
authors.forename: Jose A
authors.initials: JA
authors.lastname: Salado-Jimena
authors.forename: Helena
authors.initials: H
authors.lastname: Löf
authors.forename: Ido J
authors.initials: IJ
authors.lastname: Bontekoe
authors.forename: Connie
authors.initials: C
authors.lastname: Nielsen
authors.forename: Caroline
authors.initials: C
authors.lastname: Verheggen
authors.forename: Geert
authors.initials: G
authors.lastname: van Waeg
authors.forename: Pieter F
authors.initials: PF
authors.lastname: van der Meer
authors.forename: Dirk
authors.initials: D
authors.lastname: de Korte
authors.forename: Morten B
authors.initials: MB
authors.lastname: Hansen
authors.forename: Folke
authors.initials: F
authors.lastname: Knutson
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D001772:Blood Cell Count; D001792:Blood Platelets / Q000378:metabolism / Q000502:physiology; D001793:Blood Preservation / Q000379:methods; D001800:Blood Specimen Collection / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D015925:Cryopreservation; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D006801:Humans; D047589:Leukocyte Reduction Procedures; D008297:Male; D008875:Middle Aged; D010949:Plasma / Q000378:metabolism; D015539:Platelet Activation
chemical_list: D015415:Biomarkers