title: [Identification and preliminary analysis of a novel full-length cDNA encoding retinoid X receptor 2 from Schistosoma japonicum].
abstract: OBJECTIVE
  To clone and preliminarily analyze the full-length cDNA encoding retinoid X receptor 2 (RXR2) from Schistosoma japonicum.
  
  
  METHODS
  The rapid amplification cDNA ends (RACE)was applied to get a full-length cDNA encoding retinoid X receptor 2 from S. japonicum (SjRXR2). The transcription of SjRXR2 was detected by real-time PCR. By bioinformatical technology, the gene structure was analyzed and the antibody epitope was predicted. The polyclonal antibodies were raised in mice immunized with the synthesis peptide. Western blot was applied to detect its expression in the worm.
  
  
  RESULTS
  The full-length cDNA of SjRXR2 was 5 960 bp and contained an open reading frame encoding a 1 435 amino acid which had a predicted molecular weight 159 kDa. Bioinformatical analysis indicated that SjRXR2 had a highly conserved DNA binding domain (DBD) and a moderate conserved ligand binding domain (LBD). The relative mRNA (s) of SjRXR2 with higher expressions at Day 21 and 42 were evaluated in five different S. japonicum developmental stages. The Western blot analysis showed that polyclonal antibodies were able to specifically recognize the protein with molecular around 150 kDa from the extract of S. japonicum.
  
  
  CONCLUSIONS
  A full-length cDNA encoding retinoid X receptor 2 (RXR2) from S. japonicum is obtained which provides preliminary information for further investigation of SjRXR2 functions in S. japonicum.
authors.affiliation: School of Life Science, Xiamen University, Xiamen 361005, China.
authors.forename: Chun-Hui
authors.initials: CH
authors.lastname: Qiu
authors.forename: Sheng-Fa
authors.initials: SF
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Hong
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Fu
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Mei-Mei
authors.initials: MM
authors.lastname: Wei
authors.forename: De-Zhou
authors.initials: DZ
authors.lastname: Ai
authors.forename: Jiao-Jiao
authors.initials: JJ
authors.lastname: Lin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011817:Rabbits; D047488:Retinoid X Receptors / Q000235:genetics* / Q000502:physiology; D012549:Schistosoma japonicum / Q000502:physiology*
chemical_list: D018076:DNA, Complementary; D047488:Retinoid X Receptors