title: Cell therapy with bone marrow mononuclear cells in elastase-induced pulmonary emphysema.
abstract: Emphysema is characterized by destruction of alveolar walls with loss of gas exchange surface and consequent progressive dyspnea. This study aimed to evaluate the efficiency of cell therapy with bone marrow mononuclear cells (BMMC) in an animal model of elastase-induced pulmonary emphysema. Emphysema was induced in C57Bl/J6 female mice by intranasal instillation of elastase. After 21 days, the mice received bone marrow mononuclear cells from EGFP male mice with C57Bl/J6 background. The groups were assessed by comparison and statistically significant differences (p < 0.05) were observed among the groups treated with BMMC and evaluated after 7, 14 and 21 days. Analysis of the mean linear intercept (Lm) values for the different groups allowed to observe that the group treated with BMMC and evaluated after 21 days showed the most significant result. The group that received no treatment showed a statistically significant difference when compared to other groups, except the group treated and evaluated after 21 days, evidencing the efficacy of cell therapy with BMMC in pulmonary emphysema.
authors.affiliation: Inter-units Program of Post-Graduation in Biotechnology, University of São Paulo, Butantan Inst.-IPT, São Paulo, SP, Brazil.
authors.forename: Nathalia
authors.initials: N
authors.lastname: Longhini-Dos-Santos
authors.forename: Valter Abraão
authors.initials: VA
authors.lastname: Barbosa-de-Oliveira
authors.forename: Rodrigo Heras
authors.initials: RH
authors.lastname: Kozma
authors.forename: Carolina Arruda de
authors.initials: CA
authors.lastname: Faria
authors.forename: Talita
authors.initials: T
authors.lastname: Stessuk
authors.forename: Fernando
authors.initials: F
authors.lastname: Frei
authors.forename: João Tadeu
authors.initials: JT
authors.lastname: Ribeiro-Paes
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology*; D016026:Bone Marrow Transplantation; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology / Q000276:immunology; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology / Q000637:transplantation*; D008168:Lung / Q000276:immunology / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010196:Pancreatic Elastase; D011656:Pulmonary Emphysema / Q000139:chemically induced* / Q000276:immunology / Q000473:pathology / Q000628:therapy*
chemical_list: D015415:Biomarkers; D010196:Pancreatic Elastase