title: Thyroidal effects of di-(2-ethylhexyl) phthalate in rats of different selenium status.
abstract: This study was designed to investigate the effects of di-(2-ethylhexyl) phthalate (DEHP) on thyroid hormone levels and oxidant/antioxidant parameters in the rat and to evaluate the effects of selenium status. Selenium deficiency was produced by feeding 3-week-old Sprague-Dawley rats with <0.05 mg selenium/kg body weight for 5 weeks, and the supplementation group received a diet of 1 mg selenium/kg body weight. DEHP-treated groups received the compound at a dose of 1000 mg/kg by gavage during the last 10 days of the feeding period. Levels of thyroid hormone levels as well as selenoenzyme (glutathione peroxidase 1, thioredoxin reductase), catalase, and superoxide dismutase (SOD) activity and thiobarbituric acid reactive substance (TBARS) were measured. Total thyroxine (TT4) levels decreased significantly with DEHP exposure (~25%), whereas TT3 level was not altered. The TT4 lowering effect of DEHP exposure was not affected by selenium deficiency but was observed when animals exposed to DEHP received a selenium supplement. DEHP was found to alter the antioxidant status and induce oxidative stress in rat thyroid by increasing SOD activity (~30%) and TBARS levels (~35%). The effects of DEHP were much more pronounced in selenium-deficient rats, as evidenced by significant increases in SOD activity (~65%) and TBARS levels (~55%) compared with the control levels. Thus, these results show the thyroid-disrupting effect of DEHP in rats and protection by selenium.
authors.affiliation: Faculty of Pharmacy, Department of Toxicology, Hacettepe University, Ankara, Turkey.
authors.forename: Pinar
authors.initials: P
authors.lastname: Erkekoglu
authors.forename: Belma Kocer
authors.initials: BK
authors.lastname: Giray
authors.forename: Murat
authors.initials: M
authors.lastname: Kizilgun
authors.forename: Isabelle
authors.initials: I
authors.lastname: Hininger-Favier
authors.forename: Walid
authors.initials: W
authors.lastname: Rachidi
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Roussel
authors.forename: Alain
authors.initials: A
authors.lastname: Favier
authors.forename: Filiz
authors.initials: F
authors.lastname: Hincal
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D019587:Dietary Supplements; D004051:Diethylhexyl Phthalate / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D023421:Models, Animal; D018384:Oxidative Stress / Q000187:drug effects; D010968:Plasticizers / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000172:deficiency / Q000378:metabolism* / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013961:Thyroid Gland / Q000187:drug effects* / Q000378:metabolism*; D013963:Thyroid Hormones / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010968:Plasticizers; D017392:Thiobarbituric Acid Reactive Substances; D013963:Thyroid Hormones; D004051:Diethylhexyl Phthalate; D013482:Superoxide Dismutase; D012643:Selenium