title: MicroRNA-205 directly regulates the tumor suppressor, interleukin-24, in human KB oral cancer cells.
abstract: MicroRNA (miRNA) is a form of small noncoding RNA that regulates the expression of genes either by inhibiting mRNA translation or by inducing its degradation. Small microRNA play important roles in regulating a large number of cellular processes, including development, proliferation and apoptosis. This study examined the biological functions of miR-205 as a tumor suppressor in KB oral cancer cells. The results showed that miR-205 expression was significantly lower in KB oral cancer cells than in human normal oral keratinocytes. Furthermore, the miR-205 over-expressed in KB oral cancer cells increased the cell cytotoxicity and induced apoptosis through the activation of caspase-3/-7. The transfection of miR-205 into KB oral cancer cells strongly induced IL-24, a well known cytokine that acts as a tumor suppressor in a range of tumor tissues. In addition, miR-205 targeted the IL-24 promoter directly to induce gene expression. Overall, miR-205 has significant therapeutic potential to turn on silenced tumor suppressor genes by targeting them with miRNA.
authors.affiliation: Oral Biology Research Institute, Chosun University School of Dentistry, Gwangju, Korea.
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Kim
authors.forename: Sun-Kyoung
authors.initials: SK
authors.lastname: Yu
authors.forename: Myoung-Hwa
authors.initials: MH
authors.lastname: Lee
authors.forename: Min-Gyeong
authors.initials: MG
authors.lastname: Park
authors.forename: Euteum
authors.initials: E
authors.lastname: Park
authors.forename: Su-Gwan
authors.initials: SG
authors.lastname: Kim
authors.forename: Sook-Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Chun Sung
authors.initials: CS
authors.lastname: Kim
authors.forename: Heung-Joong
authors.initials: HJ
authors.lastname: Kim
authors.forename: Hong Sung
authors.initials: HS
authors.lastname: Chun
authors.forename: Sang-Woo
authors.initials: SW
authors.lastname: Chun
authors.forename: Do Kyung
authors.initials: DK
authors.lastname: Kim
mesh_terms: D017209:Apoptosis; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D020169:Caspases / Q000378:metabolism; D049109:Cell Proliferation; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D007378:Interleukins / Q000235:genetics* / Q000378:metabolism; D007624:KB Cells; D008156:Luciferases / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D000099180:Interleukin-24
chemical_list: D014408:Biomarkers, Tumor; D020169:Caspases; D007378:Interleukins; D008156:Luciferases; D035683:MicroRNAs; D012333:RNA, Messenger; D000099180:Interleukin-24; C534425:MIR205, human