title: Aqueous extract of Mosla chinensis inhibits mast cell-mediated allergic inflammation.
abstract: Allergic inflammatory diseases such as food allergy, asthma, sinusitis, and atopic dermatitis are increasing worldwide. In this study, we investigated the effects of aqueous extract of Mosla chinensis Max. (AMC) on mast cell-mediated allergic inflammation and studied the possible mechanism of this action. AMC inhibited compound 48/80-induced systemic and immunoglobulin E (IgE)-mediated local anaphylaxis. AMC reduced intracellular calcium levels and downstream histamine release from rat peritoneal mast cells activated by compound 48/80 or IgE. In addition, AMC decreased gene expression and secretion of proinflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-8 in human mast cells. The inhibitory effect of AMC on cytokine expression was nuclear factor (NF)-κB dependent. Our results indicate that AMC inhibits mast cell-mediated allergic inflammatory reaction by suppressing histamine release and expression of proinflammatory cytokines and the involvement of calcium and NF-κB in these effects. AMC might be a possible therapeutic candidate for allergic inflammatory disorders.
authors.affiliation: CMRI, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 700-422,  Republic of Korea.
authors.forename: Hui-Hun
authors.initials: HH
authors.lastname: Kim
authors.forename: Jin-Su
authors.initials: JS
authors.lastname: Yoo
authors.forename: Tae-Yong
authors.initials: TY
authors.lastname: Shin
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000818:Animals; D015153:Blotting, Western; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006636:Histamine Release / Q000187:drug effects; D006967:Hypersensitivity / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control*; D007249:Inflammation / Q000276:immunology / Q000517:prevention & control*; D018836:Inflammation Mediators / Q000378:metabolism; D019686:Lamiaceae / Q000737:chemistry*; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D010936:Plant Extracts