title: Magnesium incorporation into β-TCP reduced its in vivo resorption by decreasing parathormone production.
abstract: Beta-tricalcium phosphate (β-TCP), one of the most widely used bioresorbable materials for bone therapy, can be doped with magnesium ions, generating β-TCMP. The objectives of this work were to evaluate, on a murine dental alveolus grafting model, the biocompatibility of β-TCP and β-TMCP granules by histomorphometric analysis, as well as the impact on plasmatic levels of receptor activator of nuclear factor κB ligand (RANK-L), osteoprotegerin (OPG), osteocalcin, osteopontin, and parathormone (PTH) during bone repair, using Luminex multiplexing technology. After grafting for 42 days, β-TCP grafted group presented higher bioresorption and induced more newly formed bone than β-TCMP (p < 0.05). β-TCP grafting also induced higher plasmatic levels of RANK-L, compared to β-TCMP and control (blood clot) groups at 21st day (p < 0.05). PTH, which remained at low levels in control group, presented a time-dependent increase in grafted groups, attaining significantly higher levels with β-TCP by the 42nd day (p < 0.05). RANK-L/OPG ratio increased on β-TCP group and attained a peak on the 21st day. In conclusion, β-TCP granules were more bioresorbable and osteogenic than β-TCMP granules, and the resorption of both materials might have been affected by osteoclastogenesis modulated by changes in the plasmatic levels of PTH and RANK-L.
authors.affiliation: Department of Metallurgy and Materials, Federal University of Rio de Janeiro/COPPE, Rio de Janeiro, RJ, Brazil.
authors.forename: Debora H
authors.initials: DH
authors.lastname: Yassuda
authors.forename: Neusa F M
authors.initials: NF
authors.lastname: Costa
authors.forename: Gustavo O
authors.initials: GO
authors.lastname: Fernandes
authors.forename: Gutemberg G
authors.initials: GG
authors.lastname: Alves
authors.forename: José M
authors.initials: JM
authors.lastname: Granjeiro
authors.forename: Glória de A
authors.initials: Gde A
authors.lastname: Soares
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry* / Q000494:pharmacology*; D003460:Crystallization; D007118:Immunoassay; D008274:Magnesium / Q000737:chemistry* / Q000494:pharmacology*; D008422:Materials Testing; D015675:Osteocalcin / Q000097:blood; D010012:Osteogenesis; D053495:Osteopontin / Q000097:blood; D053244:Osteoprotegerin / Q000097:blood; D010281:Parathyroid Hormone / Q000096:biosynthesis*; D011208:Powders; D053245:RANK Ligand / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D020390:Tooth Socket; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D053244:Osteoprotegerin; D010281:Parathyroid Hormone; D011208:Powders; D053245:RANK Ligand; C485817:beta-tricalcium phosphate; D015675:Osteocalcin; D053495:Osteopontin; D008274:Magnesium