title: [Determination of nine kinds of polycyclic aromatic hydrocarbons in serum by high performance liquid chromatography].
abstract: OBJECTIVE
  To develop a high performance liquid chromatography (LC) method for simultaneously determination of polycyclic aromatic hydrocarbons (PAHs) in serum.
  
  
  METHODS
  PAHs could be separated completely by using C18 column and detected by fluorescence detector at wavelengths (Ex = 340 nm, Em = 425 nm). The mobile phase A was acetonitrile and mobile phase B was water with a flow rate at 1.0 ml/min.
  
  
  RESULTS
  The concentration of PAHs achieved a good linear relation with the peak area. The correlation coefficient was in the range of 0.9941-0.9999. The recoveries were in the range of 91.4%-99.8% and the RSD were in the range of 2.56%-12.7%. The detection limits were in the range of 0.05-0.10 ng/ml.
  
  
  CONCLUSIONS
  The method was simple, rapid, sensitive and accurate, and suitable for the detection of PAHs in serum.
authors.affiliation: Institute for Environment Hygiene and Health Related Product Safety, Chinese CDC, Beijing 100021, China.
authors.forename: Changming
authors.initials: C
authors.lastname: Ding
authors.forename: Lei
authors.initials: L
authors.lastname: Zheng
authors.forename: Shaobin
authors.initials: S
authors.lastname: Lin
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004781:Environmental Exposure; D004785:Environmental Pollutants / Q000097:blood*; D006801:Humans; D011084:Polycyclic Aromatic Hydrocarbons / Q000097:blood*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D004785:Environmental Pollutants; D011084:Polycyclic Aromatic Hydrocarbons