title: Biophysical characterization of membrane proteins in nanodiscs.
abstract: Nanodiscs are self-assembled discoidal phospholipid bilayers surrounded and stabilized by membrane scaffold proteins (MSPs), that have become a powerful and promising tool for the study of membrane proteins. Even though their reconstitution is highly regulated by the type of MSP and phospholipid input, a biophysical characterization leading to the determination of the stoichiometry of MSP, lipid and membrane protein is essential. This is important for biological studies, as the oligomeric state of membrane proteins often correlates with their functional activity. Typically combinations of several methods are applied using, for example, modified samples that incorporate fluorescent labels, along with procedures that result in nanodisc disassembly and lipid dissolution. To obtain a comprehensive understanding of the native properties of nanodiscs, modification-free analysis methods are required. In this work we provide a strategy, using a combination of dynamic light scattering and analytical ultracentrifugation, for the biophysical characterization of unmodified nanodiscs. In this manner we characterize the nanodisc preparation in terms of its overall polydispersity and characterize the hydrodynamically resolved nanodisc of interest in terms of its sedimentation coefficient, Stokes' radius and overall protein and lipid stoichiometry. Functional and biological applications are also discussed for the study of the membrane protein embedded in nanodiscs under defined experimental conditions.
authors.affiliation: Membrane Protein Structure Function Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 5625 Fishers Lane, Room 4S12, Rockville, MD 20852, USA. inagakisn@ninds.nih.gov
authors.forename: Sayaka
authors.initials: S
authors.lastname: Inagaki
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Ghirlando
authors.forename: Reinhard
authors.initials: R
authors.lastname: Grisshammer
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000235:genetics; D057446:Hydrodynamics; D008051:Lipid Bilayers / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry*; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D018028:Receptors, Neurotensin / Q000737:chemistry*; D014461:Ultracentrifugation / Q000379:methods
chemical_list: D008051:Lipid Bilayers; D008565:Membrane Proteins; D010743:Phospholipids; D018028:Receptors, Neurotensin; C114036:neurotensin type 1 receptor