title: [Determination of five microcystins in drinking water by HPLC/MS/MS].
abstract: OBJECTIVE
  A high performance liquid chromatography-tandem mass spectrometric method was established for determination of five microcystins( MC-LR,MC-LW,MC-RR, MC-LF, MC-YR)in drinking water and source water.
  
  
  METHODS
  The five microcystins in water was cleaned by 0.22 microm millipore filter, then detected by high performance liquid chromatography-tandem mass spectrometry. Identification was achieved by electrospray ionization (ESI) in positive mode using multiple reaction monitoring.
  
  
  RESULTS
  The calibration curves of five microcystins showed good linearity in the range of 0.5-50 microg/L with correlation coefficient in the range of 0.9994 -1.0000. The detection limit of the method was from 0.06 microg/L to 0.08 microg/L, the recoveries of two spiking levels ranged from 91.2% to 102%, and RSDs of range from 2.11% to 3.26% were obtained.
  
  
  CONCLUSIONS
  The method for determination of five microcystins in drinking water and source water by HPLC-MS/MS was of operation convenience, less interference from impurities and good accuracy, which could meet the requirements of national health standard method for the determination of microcystins in drinking water.
authors.affiliation: Shenzhen Center for Disease Control & Prevention, Shenzhen 518055, China. liuhonghe@sina.com
authors.forename: Honghe
authors.initials: H
authors.lastname: Liu
authors.forename: Lisha
authors.initials: L
authors.lastname: Mao
authors.forename: Zhou
authors.initials: Z
authors.lastname: Zhu
authors.forename: Guihua
authors.initials: G
authors.lastname: Liu
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Chen
mesh_terms: D002273:Carcinogens / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D060766:Drinking Water / Q000032:analysis*; D052998:Microcystins / Q000032:analysis*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods; D014873:Water Pollutants / Q000032:analysis*
chemical_list: D002273:Carcinogens; D060766:Drinking Water; D052998:Microcystins; D014873:Water Pollutants