title: A noncanonical postsynaptic transport route for a GPCR belonging to the serotonin receptor family.
abstract: Postsynaptic receptor trafficking plays an essential role in tuning neurotransmission and signal plasticity and has emerged as a potential therapeutic target in neuropsychiatric disease. Using a novel application of fluorescence recovery after photobleaching in rat hippocampal neurons, we examined transport from the soma to dendrites of seven G-protein-coupled receptors (GPCRs) implicated in mood disorders. Most GPCRs were delivered to dendrites via lateral diffusion, but one GPCR, the serotonin 1B receptor (5-HT(1B)), was delivered to the dendrites in secretory vesicles. Within the dendrites, 5-HT(1B) were stored in a reservoir of accessible vesicles that were recruited to preferential sites in plasma membrane, as observed with superecliptic pHluorin labeling. After membrane recruitment, 5-HT(1B) transport via lateral diffusion and temporal confinement to inhibitory and excitatory synapses was monitored by single particle tracking. These results suggest an alternative mechanism for control of neuronal activity via a GPCR that has been implicated in mood regulation.
authors.affiliation: Department of Women's and Children's Health, Karolinska Institutet, 17177 Stockholm, Sweden.
authors.forename: Thomas
authors.initials: T
authors.lastname: Liebmann
authors.forename: Markus
authors.initials: M
authors.lastname: Kruusmägi
authors.forename: Nermin
authors.initials: N
authors.lastname: Sourial-Bassillious
authors.forename: Alexander
authors.initials: A
authors.lastname: Bondar
authors.forename: Per
authors.initials: P
authors.lastname: Svenningsson
authors.forename: Marc
authors.initials: M
authors.lastname: Flajolet
authors.forename: Paul
authors.initials: P
authors.lastname: Greengard
authors.forename: Lena
authors.initials: L
authors.lastname: Scott
authors.forename: Hjalmar
authors.initials: H
authors.lastname: Brismar
authors.forename: Anita
authors.initials: A
authors.lastname: Aperia
mesh_terms: D000818:Animals; D006624:Hippocampus / Q000378:metabolism*; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D018613:Microscopy, Confocal; D009474:Neurons / Q000378:metabolism*; D021381:Protein Transport / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D011985:Receptors, Serotonin / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013570:Synaptic Membranes / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology*
chemical_list: D043562:Receptors, G-Protein-Coupled; D011985:Receptors, Serotonin