title: Role of low voltage activated calcium channels in neuritogenesis and active migration of embryonic neural progenitor cells.
abstract: The central role of calcium influx and electrical activity in embryonic development raises important questions about the role and regulation of voltage-dependent calcium influx. Using cultured neural progenitor cell (NPC) preparations, we recorded barium currents through voltage-activated channels using the whole-cell configuration of the patch-clamp technique and monitored intracellular free calcium concentrations with Fura-2 digital imaging. We found that NPCs as well as expressing high-voltage-activated (HVA) calcium channels express functional low-threshold voltage-dependent calcium channels in the very early stages of differentiation (5 h to 1 day). The size of the currents recorded at -50 versus -20 mV after 1 day in differentiation was dependent on the nature of the charge carrier. Peak currents measured at -20 mV in the presence 10 mM Ca2+ instead of 10 mM Ba2+ had a tendency to be smaller, whereas the nature of the divalent species did not influence the amplitude measured at -50 mV. The T-type channel blockers mibefradil and NNC 55-0396 significantly reduced the calcium responses elicited by depolarizing with extracellular potassium, while the overall effect of the HVA calcium channel blockers was small at differentiation day 1. At differentiation day 20, the calcium responses were effectively blocked by nifedipine. Time-lapse imaging of differentiating neurospheres cultured in the presence of low-voltage-activated (LVA) blockers showed a significant decrease in the number of active migrating neuron-like cells and neurite extensions. Together, these data provide evidence that LVA calcium channels are involved in the physiology of differentiating and migrating NPCs.
authors.affiliation: Biomedicum Helsinki, Institute of Biomedicine/Physiology, University of Helsinki, Helsinki, Finland. lauri.louhivuori@helsinki.fi
authors.forename: Lauri M
authors.initials: LM
authors.lastname: Louhivuori
authors.forename: Verna
authors.initials: V
authors.lastname: Louhivuori
authors.forename: Henna-Kaisa
authors.initials: HK
authors.lastname: Wigren
authors.forename: Elina
authors.initials: E
authors.lastname: Hakala
authors.forename: Linda C
authors.initials: LC
authors.lastname: Jansson
authors.forename: Tommy
authors.initials: T
authors.lastname: Nordström
authors.forename: Maija L
authors.initials: ML
authors.lastname: Castrén
authors.forename: Karl E
authors.initials: KE
authors.lastname: Akerman
mesh_terms: D000818:Animals; D001464:Barium / Q000378:metabolism / Q000502:physiology; D001562:Benzimidazoles / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020746:Calcium Channels, L-Type / Q000378:metabolism / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D003521:Cyclopropanes / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000378:metabolism / Q000502:physiology*; D016257:Fura-2 / Q000737:chemistry; D008564:Membrane Potentials / Q000187:drug effects; D020748:Mibefradil / Q000494:pharmacology; D051379:Mice; D018613:Microscopy, Confocal; D009281:Naphthalenes / Q000494:pharmacology; D058953:Neural Stem Cells / Q000378:metabolism / Q000502:physiology*; D016501:Neurites / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D009543:Nifedipine / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D059008:Time-Lapse Imaging
chemical_list: D001562:Benzimidazoles; D002121:Calcium Channel Blockers; D020746:Calcium Channels, L-Type; D003521:Cyclopropanes; D009281:Naphthalenes; C484287:NNC 55-0396; D001464:Barium; D020748:Mibefradil; D009543:Nifedipine; D002118:Calcium; D016257:Fura-2