title: A comparison of tissue-engineered bone from adipose-derived stem cell with autogenous bone repair in maxillary alveolar cleft model in dogs.
abstract: This study was designed to compare bone regeneration of tissue-engineered bone from adipose-derived stem cell and autogenous bone graft in a canine maxillary alveolar cleft model. In this prospective clinical trial, mesenchymal stem cells (MSCs) were isolated from subcutaneous canine adipose tissue. Undifferentiated cells were incubated with a 3mm×3mm×3mm hydroxyapatite/beta-tricalcium phosphate scaffold, in specific osteogenic medium for 21 days. Four mongrel dogs were prepared by removal of two of the three incisors bilaterally and a 15mm defect in bone was created from crest to nasal floor. After healing, repair was followed by a tissue engineered bone graft from adipose-derived stem cells on one side and corticocancellous tibial auto graft on the other side. Bone regeneration was evaluated by histomorphometry on days 15 and 60 after implantation. The data were analysed with descriptive and t test methods (α=0.05). Bone formation on the autograft sides was higher than on the stem cell sides at 15 and 60 days, 45% and 96% versus 5% and 70%, respectively. Differences between the two groups at 15 and 60 days were significant (p=0.004 and 0.001, respectively). Although autograft is still the gold standard for bone regeneration, tissue engineered bone may provide an acceptable alternative.
authors.affiliation: Department of Maxillofacial Surgery, Azahra Hospital, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: N
authors.initials: N
authors.lastname: Pourebrahim
authors.forename: B
authors.initials: B
authors.lastname: Hashemibeni
authors.forename: S
authors.initials: S
authors.lastname: Shahnaseri
authors.forename: N
authors.initials: N
authors.lastname: Torabinia
authors.forename: B
authors.initials: B
authors.lastname: Mousavi
authors.forename: S
authors.initials: S
authors.lastname: Adibi
authors.forename: F
authors.initials: F
authors.lastname: Heidari
authors.forename: M Jafary
authors.initials: MJ
authors.lastname: Alavi
mesh_terms: D000541:Alveolectomy / Q000379:methods; D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D001861:Bone Regeneration / Q000502:physiology; D016025:Bone Transplantation / Q000379:methods / Q000473:pathology*; D002113:Calcification, Physiologic / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D002469:Cell Separation; D002972:Cleft Palate / Q000601:surgery*; D003094:Collagen / Q000032:analysis; D003470:Culture Media; D004195:Disease Models, Animal; D004285:Dogs; D017886:Durapatite / Q000737:chemistry; D005434:Flow Cytometry; D008437:Maxilla / Q000601:surgery*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000473:pathology; D010012:Osteogenesis / Q000502:physiology; D011446:Prospective Studies; D050151:Subcutaneous Fat / Q000166:cytology*; D013977:Tibia / Q000601:surgery; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry; D060053:Transplant Donor Site / Q000601:surgery; D014182:Transplantation, Autologous
chemical_list: D003470:Culture Media; D003094:Collagen; D017886:Durapatite