title: Lewis y antigen is expressed in oral squamous cell carcinoma cell lines and tissues, but disappears in the invasive regions leading to the enhanced malignant properties irrespective of sialyl-Lewis x.
abstract: Expression and implication of carbohydrate antigens in squamous cell carcinomas (SCCs) in oral cavity was examined. In the cell lines, type 2H and Lewis y antigens were markedly expressed. In the tissues from SCC patients and benign disorders, type 2H was highly expressed in hyperplasia (96.4 %), displasia (92.9 %) and SCC (100 %). Lewis y was, in turn, expressed mainly in cancer tissues (91.3 %), suggesting that Lewis y is a cancer-associated antigen. Normal oral mucosa showed no expression of these blood group antigens. Surprisingly, Lewis y antigen disappeared in the invasion sites where Ki-67 was definitely stained. Over-expression of Lewis y with manipulation of a fucosyltransferase cDNA resulted in suppression of cell growth and invasion, and knockdown of Lewis y also brought about increased cell growth and invasion. In either situations, no changes in the expression of sialyl-Lewis x could be found. Lowered tumor growth and invasion into surrounding tissues were also shown in Lewis y-positive SCC grafts in nu/nu mice. All these results together with alternative staining between Lewis y and Ki-67 in cancer tissues and FUT1 transfectants suggested that loss of Lewis y is a crucial event for the late stage of SCCs.
authors.affiliation: Department of Biochemistry II, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya, 466-0065, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hotta
authors.forename: Kazunori
authors.initials: K
authors.lastname: Hamamura
authors.forename: Kyoko
authors.initials: K
authors.lastname: Yamashita
authors.forename: Hidenobu
authors.initials: H
authors.lastname: Shibuya
authors.forename: Noriyo
authors.initials: N
authors.lastname: Tokuda
authors.forename: Noboru
authors.initials: N
authors.lastname: Hashimoto
authors.forename: Keiko
authors.initials: K
authors.lastname: Furukawa
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Hisashi
authors.initials: H
authors.lastname: Hattori
authors.forename: Shinya
authors.initials: S
authors.lastname: Toyokuni
authors.forename: Minoru
authors.initials: M
authors.lastname: Ueda
authors.forename: Koichi
authors.initials: K
authors.lastname: Furukawa
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D045744:Cell Line, Tumor; D005647:Fucosyltransferases / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D019394:Ki-67 Antigen / Q000235:genetics / Q000378:metabolism; D007983:Lewis Blood Group Antigens / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009061:Mouth Mucosa / Q000378:metabolism / Q000473:pathology; D009062:Mouth Neoplasms / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D009844:Oligosaccharides / Q000235:genetics / Q000378:metabolism*; D009928:Organ Specificity; D000080506:Sialyl Lewis X Antigen; D000097631:Galactoside 2-alpha-L-fucosyltransferase
chemical_list: D005647:Fucosyltransferases; D019394:Ki-67 Antigen; D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; D000097631:Galactoside 2-alpha-L-fucosyltransferase; C094162:Lewis Y antigen