title: [Study on anti-cataract effect of gigantol combined with syringic acid and their mechanism].
abstract: OBJECTIVE
  To study the anti-cataract effect of gigantol combined with syringic acid and their action mechanism.
  
  
  METHODS
  H202-induced lens oxidative injury in vitro rat model was establish to observe the impact of gigantol combined with syringic acid on lens transparency under a dissecting microscope. D-galactose-induced cataract rat model was established to observe the impact of gigantol combined with syringic acid on lens transparency under a slit-lamp. UV spectrophotometry was adopted to detect the inhibitory activity of gigantol combined with syringic acid against AR. Molecular docking method was used to detect binding sites, binding types and pharmacophores of gigantol combined with syringic acid in prohibiting aldose reductase.
  
  
  RESULTS
  Both in vitro and in vivo experiments showed a good anti-sugar cataract activity in the combination of gigantol and syringic acid and a better collaborative effect than single component-gigantol and syringic acid and positive control drug Catalin. Molecular docking and dynamic simulation showed their collaborative AR-inhibiting amino acid residue was Asn160 and the major acting force was Van der Waals' force, which formed common pharmacophores.
  
  
  CONCLUSIONS
  Gigantol combined with syringic acid shows good anti-cataract, their action mechanism is reflected in their good collaborative inhibitory effect on AR.
authors.affiliation: Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, China.
authors.forename: Hongxing
authors.initials: H
authors.lastname: Diao
authors.forename: Yanqun
authors.initials: Y
authors.lastname: Yi
authors.forename: Hui
authors.initials: H
authors.lastname: Qi
authors.forename: Xinxin
authors.initials: X
authors.lastname: Gao
authors.forename: Hua
authors.initials: H
authors.lastname: Fang
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Wei
authors.forename: Qiong
authors.initials: Q
authors.lastname: Gu
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Zhengtao
authors.initials: Z
authors.lastname: Wang
authors.forename: Lianquan
authors.initials: L
authors.lastname: Gu
mesh_terms: D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors; D000818:Animals; D001632:Bibenzyls; D002386:Cataract / Q000188:drug therapy* / Q000201:enzymology; D004357:Drug Synergism; D005260:Female; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D006139:Guaiacol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000187:drug effects / Q000201:enzymology; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001632:Bibenzyls; D005707:Gallic Acid; C478099:gigantol; D006139:Guaiacol; C001945:syringic acid; D000449:Aldehyde Reductase