title: Improved analysis for determining diffusion coefficients from short, single-molecule trajectories with photoblinking.
abstract: Two maximum likelihood estimation (MLE) methods were developed for optimizing the analysis of single-molecule trajectories that include phenomena such as experimental noise, photoblinking, photobleaching, and translation or rotation out of the collection plane. In particular, short, single-molecule trajectories with photoblinking were studied, and our method was compared to existing analytical techniques applied to simulated data. The optimal method for various experimental cases was established, and the optimized MLE method was applied to a real experimental system: single-molecule diffusion of fluorescent molecular machines known as nanocars.
authors.affiliation: Department of Chemistry and Rice Quantum Institute, Rice University, Houston, Texas 77251-1892, United States.
authors.forename: Bo
authors.initials: B
authors.lastname: Shuang
authors.forename: Chad P
authors.initials: CP
authors.lastname: Byers
authors.forename: Lydia
authors.initials: L
authors.lastname: Kisley
authors.forename: Lin-Yung
authors.initials: LY
authors.lastname: Wang
authors.forename: Julia
authors.initials: J
authors.lastname: Zhao
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Morimura
authors.forename: Stephan
authors.initials: S
authors.lastname: Link
authors.forename: Christy F
authors.initials: CF
authors.lastname: Landes
mesh_terms: D004058:Diffusion; D005453:Fluorescence; D049329:Nanostructures / Q000737:chemistry*; D038761:Photobleaching