title: CYP17 and CYP19 genetic variants are not associated with age at natural menopause in Polish women.
abstract: The aim of the study was to investigate associations between two common polymorphisms of CYP17 and CYP19, encoding key enzymes of estrogen biosynthesis, and age at menopause in Polish women. One hundred fifty women after menopause (49.5±3.8 years), with no previous history of hormone replacement therapy took part in the study. The genetic control group consisted of 150 newborns from the same population. We investigated an association between the age at menopause and the single nucleotide polymorphism T→C in the 5' untranslated region (promoter) of the CYP17 gene (c.-34T>C; rs743572 - MspA1) or the number of tetranucleotide repeats [TTTA](n) (rs60271534) including deletion/insertion (D/I) of a 3 bp sequence in intron 4 of the CYP19 gene. CYP17 polymorphism was analyzed by PCR-RFLP and CYP19 by PCR and capillary electrophoresis. In the case of CYP17 polymorphism, 28.7% and 36.7% wild homozygous (TT), 50.7% and 46.0% heterozygous (TC), as well as 20.6% and 17.3% mutated homozygous (CC) types were identified in the subjects and controls, respectively. The frequency of mutated alleles (C) was 46.0% vs. 40.3% (p=0.19). In the case of CYP19 polymorphism, 34.0% and 32.0% of homozygotes (1_1), 50.7% and 51.3% of heterozygotes (1_2), 15.3% and 16.7% of homozygotes (2_2) were identified in the subjects and controls, respectively. No association between the studied CYP17 or CYP19 polymorphisms and age at menopause was found in Polish women.
authors.affiliation: Department of Histology and Developmental Biology, Pomeranian Medical University, Szczecin, Poland. brumian@pum.edu.pl
authors.forename: Bogdan
authors.initials: B
authors.lastname: Rumianowski
authors.forename: Grażyna
authors.initials: G
authors.lastname: Adler
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Safranow
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Brodowska
authors.forename: Beata
authors.initials: B
authors.lastname: Karakiewicz
authors.forename: Sylwia
authors.initials: S
authors.lastname: Słuczanowska-Głąbowska
authors.forename: Beata
authors.initials: B
authors.lastname: Łoniewska
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Piasecka
authors.forename: Andrzej
authors.initials: A
authors.lastname: Ciechanowicz
authors.forename: Maria
authors.initials: M
authors.lastname: Laszczyńska
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging / Q000502:physiology*; D001141:Aromatase / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006801:Humans; D008593:Menopause / Q000235:genetics / Q000502:physiology*; D008875:Middle Aged; D011044:Poland; D011110:Polymorphism, Genetic / Q000502:physiology*; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics / Q000378:metabolism*
chemical_list: D001141:Aromatase; D013254:Steroid 17-alpha-Hydroxylase