title: Determination of sulpiride in pharmaceutical preparations and biological fluids using a Cr (III) enhanced chemiluminescence method.
abstract: A highly sensitive and simple method for identifying sulpiride in pharmaceutical formulations and biological fluids is presented. The method is based on increased chemiluminescence (CL) intensity of a luminol-H2O2 system in response to the addition of Cr (III) under alkaline conditions. The CL intensity of the luminol-H2O2-Cr (III) system was greatly enhanced by the addition of sulpiride and the CL intensity was proportional to the concentration of sulpiride in a sample solution. Various parameters affecting the CL intensity were systematically investigated and optimized for determination of the sulpiride in a sample. Under the optimum conditions, the CL intensity was proportional to the concentration of sulpiride in the range of 0.068-4.0 µg/mL, with a good correlation coefficient of 0.997. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 8.50 × 10(-6) µg/mL and 2.83 × 10(-5) µg/mL, respectively. The method presented here produced good reproducibility with a relative standard deviation (RSD) of 2.70% (n = 7). The effects of common excipients and metal ions were studied for their interference effect. The method was validated statistically through recovery studies and successfully applied for the determination of sulpiride in pure form, pharmaceutical preparations and spiked human plasma samples. The percentage recoveries were found to range from 99.10 to 100.05% for pure form, 98.12 to 100.18% for pharmaceutical preparations and 97.9 to 101.4% for spiked human plasma.
authors.affiliation: Institute of Chemical Sciences, University of Peshawar, Peshawar, Pakistan; Department of Chemistry, Kyungpook National University, Daegu, 702-701, South Korea.
authors.forename: Muhammad Naeem
authors.initials: MN
authors.lastname: Khan
authors.forename: Muhammad Rasul
authors.initials: MR
authors.lastname: Jan
authors.forename: Jasmin
authors.initials: J
authors.lastname: Shah
authors.forename: Sang Hak
authors.initials: SH
authors.lastname: Lee
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D001826:Body Fluids / Q000737:chemistry*; D002857:Chromium / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D013469:Sulpiride / Q000032:analysis*
chemical_list: D004364:Pharmaceutical Preparations; D002857:Chromium; D008165:Luminol; D013469:Sulpiride; D006861:Hydrogen Peroxide