title: Separation, purification, and α-glucosidase inhibition of polysaccharides from Coriolus versicolor LH1 mycelia.
abstract: Intracellular polysaccharides (iPs) were separated and purified from Coriolus versicolor LH1 mycelia and characterized for their α-glucosidase inhibitory properties. Three iP fractions (iPL-F5-2-1, iPL-F5-4-1, and iPL-F5-5-1) were extracted, separated, and purified from LH1 mycelia using microwave extraction technology, a DEAE-Sepharose CL-6B column, a Diaion HP20 macroporous adsorption column, and a Sephadex™ G-50 gel-permeation column. The principal constituents of iPL-F5-2-1, iPL-F5-4-1, and iPL-F5-5-1 were saponins and polyphenoic compound mixtures. The enzyme inhibition activity, IC(50) values, of these three fractions were 1.7, 1.8, and 0.8 mg/mL, respectively. The α-glucosidase inhibitory properties were related to the presence of α-(1,4) glycosidic linkages in the polysaccharide structure and the total relative percentage of d-glucose and d-galactose in the structure of polysaccharides, other than triterpenoids.
authors.affiliation: Natural Products Chemistry Research Laboratory, Department of Bioindustry Technology, Dayeh University, Changhua 51591, Taiwan.
authors.forename: Wen-kuang
authors.initials: WK
authors.lastname: Hsu
authors.forename: Tai-hao
authors.initials: TH
authors.lastname: Hsu
authors.forename: Fang-yi
authors.initials: FY
authors.lastname: Lin
authors.forename: Yuan-kai
authors.initials: YK
authors.lastname: Cheng
authors.forename: John Po-wen
authors.initials: JP
authors.lastname: Yang
mesh_terms: D000327:Adsorption; D055453:Coriolaceae / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000737:chemistry; D065089:Glycoside Hydrolase Inhibitors; D025282:Mycelium / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D014315:Triterpenes / Q000737:chemistry; D000520:alpha-Glucosidases / Q000737:chemistry
chemical_list: D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D011134:Polysaccharides; D014315:Triterpenes; D000520:alpha-Glucosidases