title: Mutational biosynthesis of a FK506 analogue containing a non-natural starter unit.
abstract: A FK506 analogue containing a non-natural starter unit was obtained through mutasynthesis by feeding cultures of Streptomyces sp. KCTC 11604BP fkbO deletion mutant with 3-cyclohexene-1-carboxylic acid. The structure of the new compound, 32-dehydroxy-FK506, and its biological activities were determined.
authors.affiliation: Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Republic of Korea.
authors.forename: Yeon Hee
authors.initials: YH
authors.lastname: Ban
authors.forename: Jong Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Gyo Rim
authors.initials: GR
authors.lastname: Gu
authors.forename: Boram
authors.initials: B
authors.lastname: Lee
authors.forename: SangJoon
authors.initials: S
authors.lastname: Mo
authors.forename: Ho Jeong
authors.initials: HJ
authors.lastname: Kwon
authors.forename: Yeo Joon
authors.initials: YJ
authors.lastname: Yoon
mesh_terms: D053898:Biosynthetic Pathways; D002827:Chorismic Acid / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003509:Cyclohexanecarboxylic Acids / Q000737:chemistry / Q000378:metabolism; D053138:Cyclohexenes / Q000737:chemistry / Q000378:metabolism; D008956:Models, Chemical; D015394:Molecular Structure; D009154:Mutation; D007652:Oxo-Acid-Lyases / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*; D016559:Tacrolimus / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D003509:Cyclohexanecarboxylic Acids; D053138:Cyclohexenes; D010940:Plant Proteins; C004706:3-cyclohexene-1-carboxylic acid; D007652:Oxo-Acid-Lyases; C075960:chorismate pyruvate lyase; D002827:Chorismic Acid; D016559:Tacrolimus