title: Anti-obesity effect of n-3 polyunsaturated fatty acids in mice fed high-fat diet is independent of cold-induced thermogenesis.
abstract: Long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFA) exert beneficial effects on health and they could help to prevent development of obesity and associated metabolic disorders. In our previous studies in mice fed high-fat (cHF; ~60 % calories as fat) diet and maintained at 20 °C, dietary LC n-3 PUFA could counteract accretion of body fat, without inducing mitochondrial uncoupling protein 1 (UCP1) in adipose tissue, suggesting that the anti-obesity effect was not linked to adaptive (UCP1-mediated) thermogenesis. To exclude a possible dependence of the anti-obesity effect on any mechanism inducible by cold, experiments were repeated in mice maintained at thermoneutrality (30 °C). Male C57BL/6J mice were fed either cHF diet, or cHF diet supplemented with LC n-3 PUFA, or standard diet for 7 months. Similarly as at 20 °C, the LC n-3 PUFA supplementation reduced accumulation of body fat, preserved lipid and glucose homeostasis, and induced fatty acid re-esterification in epididymal white adipose tissue. Food consumption was not affected by LC n-3 PUFA intake. Our results demonstrated anti-obesity metabolic effect of LC n-3 PUFA, independent of cold-induced thermogenesis and they suggested that induction of fatty acid re-esterification creating a substrate cycle in white fat, which results in energy expenditure, could contribute to the anti-obesity effect.
authors.affiliation: Department of Adipose Tissue Biology, Institute of Physiology of the Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: P
authors.initials: P
authors.lastname: Janovská
authors.forename: P
authors.initials: P
authors.lastname: Flachs
authors.forename: L
authors.initials: L
authors.lastname: Kazdová
authors.forename: J
authors.initials: J
authors.lastname: Kopecký
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D019440:Anti-Obesity Agents; D001835:Body Weight / Q000187:drug effects; D002153:Calorimetry, Indirect; D003080:Cold Temperature; D059305:Diet, High-Fat / Q000009:adverse effects*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D015525:Fatty Acids, Omega-3 / Q000627:therapeutic use*; D005951:Glucose Tolerance Test; D006706:Homeostasis / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000188:drug therapy*; D022722:Thermogenesis / Q000187:drug effects / Q000502:physiology*; D014280:Triglycerides / Q000097:blood
chemical_list: D019440:Anti-Obesity Agents; D005230:Fatty Acids, Nonesterified; D015525:Fatty Acids, Omega-3; D014280:Triglycerides