title: In vivo broiler experiments to assess anti-Campylobacter jejuni activity of a live Enterococcus faecalis strain.
abstract: Bacterial gastroenteritis caused by thermotolerant Campylobacter species, mainly Campylobacter jejuni, has been the most reported zoonotic disease in many developed countries in recent years. Reducing Campylobacter shedding on the farm could result in a reduction of the number of campylobacteriosis cases. In 2 independent broiler seeder experiments, in which broiler chickens were orally inoculated with 2 amounts of Enterococcus faecalis MB 5259, we established whether a live E. faecalis strain was capable of reducing cecal Campylobacter colonization in broiler chickens. In previous in vitro experiments it has been demonstrated that this E. faecalis MB 5259 displays anti-Campylobacter activity. The effect of pH and bile salts on E. faecalis MB 5259 showed that growth and survival of E. faecalis MB 5259 can be impaired during passage through the gastrointestinal tract of broiler chickens. Despite these results E. faecalis MB 5259 was capable of colonizing the broiler ceca. Contrary to the in vitro experiments, in which E. faecalis MB 5259 inhibited C. jejuni MB 4185 growth, no inhibition was observed in the in vivo experiments independent of the inoculum size.
authors.affiliation: Institute for Agricultural and Fisheries Research, Technology and Food Science Unit, Brusselsesteenweg 370, Melle, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Robyn
authors.forename: G
authors.initials: G
authors.lastname: Rasschaert
authors.forename: D
authors.initials: D
authors.lastname: Hermans
authors.forename: F
authors.initials: F
authors.lastname: Pasmans
authors.forename: M
authors.initials: M
authors.lastname: Heyndrickx
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000494:pharmacology; D016123:Campylobacter jejuni / Q000502:physiology*; D002645:Chickens / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000187:drug effects / Q000502:physiology*; D005260:Female; D006863:Hydrogen-Ion Concentration; D008297:Male
chemical_list: D001647:Bile Acids and Salts