title: Phlorotannin extracts from fucales characterized by HPLC-DAD-ESI-MSn: approaches to hyaluronidase inhibitory capacity and antioxidant properties.
abstract: Purified phlorotannin extracts from four brown seaweeds (Cystoseira nodicaulis (Withering) M. Roberts, Cystoseira tamariscifolia (Hudson) Papenfuss, Cystoseira usneoides (Linnaeus) M. Roberts and Fucus spiralis Linnaeus), were characterized by HPLC-DAD-ESI-MSn. Fucophloroethol, fucodiphloroethol, fucotriphloroethol, 7-phloroeckol, phlorofucofuroeckol and bieckol/dieckol were identified. The antioxidant activity and the hyaluronidase (HAase) inhibitory capacity exhibited by the extracts were also assessed. A correlation between the extracts activity and their chemical composition was established. F. spiralis, the species presenting higher molecular weight phlorotannins, generally displayed the strongest lipid peroxidation inhibitory activity (IC₅₀ = 2.32 mg/mL dry weight) and the strongest HAase inhibitory capacity (IC₅₀ = 0.73 mg/mL dry weight). As for superoxide radical scavenging, C. nodicaulis was the most efficient species (IC₅₀ = 0.93 mg/mL dry weight), followed by F. spiralis (IC₅₀ = 1.30 mg/mL dry weight). These results show that purified phlorotannin extracts have potent capabilities for preventing and slowing down the skin aging process, which is mainly associated with free radical damage and with the reduction of hyaluronic acid concentration, characteristic of the process.
authors.affiliation: Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS-CSIC, P.O. Box 164, 30100 Campus University Espinardo, Murcia, Spain. federico@cebas.csic.es
authors.forename: Federico
authors.initials: F
authors.lastname: Ferreres
authors.forename: Graciliana
authors.initials: G
authors.lastname: Lopes
authors.forename: Angel
authors.initials: A
authors.lastname: Gil-Izquierdo
authors.forename: Paula B
authors.initials: PB
authors.lastname: Andrade
authors.forename: Carla
authors.initials: C
authors.lastname: Sousa
authors.forename: Teresa
authors.initials: T
authors.lastname: Mouga
authors.forename: Patrícia
authors.initials: P
authors.lastname: Valentão
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D044645:Fucus / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors; D020128:Inhibitory Concentration 50; D015227:Lipid Peroxidation / Q000187:drug effects; D008970:Molecular Weight; D000459:Phaeophyceae / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013481:Superoxides / Q000378:metabolism; D013634:Tannins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D013634:Tannins; D013481:Superoxides; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase