title: Global analysis of yeast mRNPs.
abstract: Proteins regulate gene expression by controlling mRNA biogenesis, localization, translation and decay. Identifying the composition, diversity and function of mRNA-protein complexes (mRNPs) is essential to understanding these processes. In a global survey of Saccharomyces cerevisiae mRNA-binding proteins, we identified 120 proteins that cross-link to mRNA, including 66 new mRNA-binding proteins. These include kinases, RNA-modification enzymes, metabolic enzymes and tRNA- and rRNA-metabolism factors. These proteins show dynamic subcellular localization during stress, including assembly into stress granules and processing bodies (P bodies). Cross-linking and immunoprecipitation (CLIP) analyses of the P-body components Pat1, Lsm1, Dhh1 and Sbp1 identified sites of interaction on specific mRNAs, revealing positional binding preferences and co-assembly preferences. When taken together, this work defines the major yeast mRNP proteins, reveals widespread changes in their subcellular location during stress and begins to define assembly rules for P-body mRNPs.
authors.affiliation: Department of Chemistry and Biochemistry, University of Colorado Boulder, Boulder, Colorado, USA.
authors.forename: Sarah F
authors.initials: SF
authors.lastname: Mitchell
authors.forename: Saumya
authors.initials: S
authors.lastname: Jain
authors.forename: Meipei
authors.initials: M
authors.lastname: She
authors.forename: Roy
authors.initials: R
authors.lastname: Parker
mesh_terms: D001665:Binding Sites; D053487:DEAD-box RNA Helicases / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D039381:RNA Cap-Binding Proteins / Q000378:metabolism; D012331:RNA, Fungal / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: C114069:LSM1 protein, S cerevisiae; C103266:PAT1 protein, S cerevisiae; D039381:RNA Cap-Binding Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D029701:Saccharomyces cerevisiae Proteins; C026452:messenger ribonucleoprotein; C445083:DHH1 protein, S cerevisiae; D053487:DEAD-box RNA Helicases