title: Increased inflammation in atherosclerotic lesions of diabetic Akita-LDLr⁻/⁻ mice compared to nondiabetic LDLr⁻/⁻ mice.
abstract: BACKGROUND
  Diabetes is associated with increased cardiovascular disease, but the underlying cellular and molecular mechanisms are poorly understood. One proposed mechanism is that diabetes aggravates atherosclerosis by enhancing plaque inflammation. The Akita mouse has recently been adopted as a relevant model for microvascular complications of diabetes. Here we investigate the development of atherosclerosis and inflammation in vessels of Akita mice on LDLr⁻/⁻ background.
  
  
  RESULTS
  Akita-LDLr⁻/⁻ and LDLr⁻/⁻ mice were fed high-fat diet from 6 to 24 weeks of age. Blood glucose levels were higher in both male and female Akita-LDLr⁻/⁻ mice (137% and 70%, resp.). Male Akita-LDLr⁻/⁻ mice had markedly increased plasma cholesterol and triglyceride levels, a three-fold increase in atherosclerosis, and enhanced accumulation of macrophages and T-cells in plaques. In contrast, female Akita-LDLr⁻/⁻ mice demonstrated a modest 29% increase in plasma cholesterol and no significant increase in triglycerides, atherosclerosis, or inflammatory cells in lesions. Male Akita-LDLr⁻/⁻ mice had increased levels of plasma IL-1β compared to nondiabetic mice, whereas no such difference was seen between female diabetic and nondiabetic mice.
  
  
  CONCLUSIONS
  Akita-LDLr⁻/⁻ mice display considerable gender differences in the development of diabetic atherosclerosis. In addition, the increased atherosclerosis in male Akita-LDLr⁻/⁻ mice is associated with an increase in inflammatory cells in lesions.
authors.affiliation: Department of Clinical Sciences Malmö, Skåne University Hospital, Lund University, CRC Entrance 72, 20502 Malmö, Sweden. daniel.engelbertsen@med.lu.se
authors.forename: Daniel
authors.initials: D
authors.lastname: Engelbertsen
authors.forename: Fong
authors.initials: F
authors.lastname: To
authors.forename: Pontus
authors.initials: P
authors.lastname: Dunér
authors.forename: Olga
authors.initials: O
authors.lastname: Kotova
authors.forename: Ingrid
authors.initials: I
authors.lastname: Söderberg
authors.forename: Ragnar
authors.initials: R
authors.lastname: Alm
authors.forename: Maria F
authors.initials: MF
authors.lastname: Gomez
authors.forename: Jan
authors.initials: J
authors.lastname: Nilsson
authors.forename: Eva
authors.initials: E
authors.lastname: Bengtsson
mesh_terms: D000818:Animals; D001011:Aorta / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D001025:Aortitis / Q000097:blood / Q000209:etiology* / Q000235:genetics / Q000276:immunology / Q000473:pathology; D050197:Atherosclerosis / Q000097:blood / Q000209:etiology* / Q000235:genetics / Q000276:immunology / Q000473:pathology; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D002478:Cells, Cultured; D002784:Cholesterol / Q000097:blood; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000150:complications* / Q000235:genetics / Q000276:immunology / Q000473:pathology; D003925:Diabetic Angiopathies / Q000097:blood / Q000209:etiology* / Q000235:genetics / Q000276:immunology / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D018836:Inflammation Mediators / Q000097:blood; D053583:Interleukin-1beta / Q000097:blood; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D058226:Plaque, Atherosclerotic; D011973:Receptors, LDL / Q000172:deficiency* / Q000235:genetics; D012737:Sex Factors; D013601:T-Lymphocytes / Q000276:immunology; D014280:Triglycerides / Q000097:blood
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D011973:Receptors, LDL; D014280:Triglycerides; D002784:Cholesterol