title: Metabolism studies of benzbromarone in rats by high performance liquid chromatography-quadrupole time of flight mass spectrometry.
abstract: A high performance liquid chromatography-quadrupole time of flight mass spectrometry (HPLC-QTOF-MS) method was employed in investigation of benzbromarone metabolites in rat plasma, urine, feces and bile samples. Meanwhile, the metabolic pathways of benzbromarone in rats were discussed. The identification was achieved on a reversed-phase C(18) column with mobile phase gradient method. The QTOF-MS was operated under full scan of MS or MS/MS in negative mode. The fragments were acquired by raising collision induced dissociation (CID) energy for speculating the structures of parent ions. According to the information from the chromatograms and mass spectra, 17 metabolites were obtained. Among them, the deoxidized phase I metabolites and an array of phase II metabolites-sulfate conjugates detected in the biological samples made the work more significant.
authors.affiliation: Department of Pharmacy, Shenyang Pharmaceutical University, 103 Wen-Hua Road, Shenyang 110016, China.
authors.forename: Haiqing
authors.initials: H
authors.lastname: Wu
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.forename: Shaojie
authors.initials: S
authors.lastname: Wang
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.forename: Xunchen
authors.initials: X
authors.lastname: Zhao
authors.forename: Fan
authors.initials: F
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001553:Benzbromarone / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005243:Feces / Q000737:chemistry; D020719:Glucuronides / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013431:Sulfates / Q000032:analysis / Q000737:chemistry / Q000378:metabolism
chemical_list: D001647:Bile Acids and Salts; D020719:Glucuronides; D013431:Sulfates; D001553:Benzbromarone