title: The fidelity of transcription: RPB1 (RPO21) mutations that increase transcriptional slippage in S. cerevisiae.
abstract: The fidelity of RNA synthesis depends on both accurate template-mediated nucleotide selection and proper maintenance of register between template and RNA. Loss of register, or transcriptional slippage, is particularly likely on homopolymeric runs in the template. Transcriptional slippage can alter the coding capacity of mRNAs and is used as a regulatory mechanism. Here we describe mutations in the largest subunit of Saccharomyces cerevisiae RNA polymerase II that substantially increase the level of transcriptional slippage. Alleles of RPB1 (RPO21) with elevated slippage rates were identified among 6-azauracil-sensitive mutants and were also isolated using a slippage-dependent reporter gene. Biochemical characterization of polymerase II isolated from these mutants confirms elevated levels of transcriptional slippage.
authors.affiliation: National Cancer Institute, Frederick National Laboratory for Cancer Research, Frederick, Maryland 21702, USA. strathej@mail.nih.gov
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Strathern
authors.forename: Francisco
authors.initials: F
authors.lastname: Malagon
authors.forename: Jordan
authors.initials: J
authors.lastname: Irvin
authors.forename: Deanna
authors.initials: D
authors.lastname: Gotte
authors.forename: Brenda
authors.initials: B
authors.lastname: Shafer
authors.forename: Maria
authors.initials: M
authors.lastname: Kireeva
authors.forename: Lucyna
authors.initials: L
authors.lastname: Lubkowska
authors.forename: Ding Jun
authors.initials: DJ
authors.lastname: Jin
authors.forename: Mikhail
authors.initials: M
authors.lastname: Kashlev
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D020134:Catalytic Domain; D002875:Chromosomes / Q000648:ultrastructure; D015966:Gene Expression Regulation, Fungal; D008958:Models, Molecular; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D009154:Mutation; D009841:Oligonucleotides / Q000235:genetics; D011485:Protein Binding; D012313:RNA / Q000378:metabolism; D012319:RNA Polymerase II / Q000235:genetics* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D009841:Oligonucleotides; D029701:Saccharomyces cerevisiae Proteins; D012313:RNA; D012319:RNA Polymerase II; C511736:RPB1 protein, S cerevisiae; D001616:beta-Galactosidase