title: SPION-enhanced magnetic resonance imaging of Alzheimer's disease plaques in AβPP/PS-1 transgenic mouse brain.
abstract: In our program to develop non-invasive magnetic resonance imaging (MRI) methods for the diagnosis of Alzheimer's disease (AD), we have synthesized antibody-conjugated, superparamagnetic iron oxide nanoparticles (SPIONs) for use as an in vivo agent for MRI detection of amyloid-β plaques in AD. Here we report studies in AβPP/PS1 transgenic mice, which demonstrate the ability of novel anti-AβPP conjugated SPIONs to penetrate the blood-brain barrier to act as a contrast agent for MR imaging of plaques. The conspicuity of the plaques increased from an average Z-score of 5.1 ± 0.5 to 8.3 ± 0.2 when the plaque contrast to noise ratio was compared in control AD mice with AD mice treated with SPIONs. The number of MRI-visible plaques per brain increased from 347 ± 45 in the control AD mice, to 668 ± 86 in the SPION treated mice. These results indicated that our SPION enhanced amyloid-β detection method delivers an efficacious, non-invasive MRI detection method in transgenic mice.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA. laurel@unm.edu
authors.forename: Laurel O
authors.initials: LO
authors.lastname: Sillerud
authors.forename: Nathan O
authors.initials: NO
authors.lastname: Solberg
authors.forename: Ryan
authors.initials: R
authors.lastname: Chamberlain
authors.forename: Robert A
authors.initials: RA
authors.lastname: Orlando
authors.forename: John E
authors.initials: JE
authors.lastname: Heidrich
authors.forename: David C
authors.initials: DC
authors.lastname: Brown
authors.forename: Christina I
authors.initials: CI
authors.lastname: Brady
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Vander Jagt
authors.forename: Michael
authors.initials: M
authors.lastname: Garwood
authors.forename: David L
authors.initials: DL
authors.lastname: Vander Jagt
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics / Q000473:pathology*; D016564:Amyloid beta-Protein Precursor / Q000235:genetics; D000818:Animals; D005290:Ferric Compounds; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D053768:Metal Nanoparticles; D051379:Mice; D008822:Mice, Transgenic; D058225:Plaque, Amyloid / Q000235:genetics / Q000473:pathology*; D053764:Presenilin-1 / Q000235:genetics
chemical_list: D016564:Amyloid beta-Protein Precursor; D005290:Ferric Compounds; D053764:Presenilin-1; C000499:ferric oxide