title: Development of a manual method for the determination of mineral oil in foods and paperboard.
abstract: So far the majority of the measurements of mineral oil saturated hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons (MOAH) were obtained from on-line high performance liquid chromatography-gas chromatography-flame ionization detection (on-line HPLC-GC-FID). Since this technique is not available in many laboratories, an alternative method with more easily available tools has been developed. Preseparation on a small conventional liquid chromatographic column was optimized to achieve robust separation between the MOSH and the MOAH, but also to keep out the wax esters from the MOAH fraction. This was achieved by mixing a small portion of silica gel with silver nitrate into highly activated silica gel and by adding toluene into the eluent for the MOAH. Toluene was also added to the MOSH fraction to facilitate reconcentration and to serve as a keeper preventing loss of volatiles during solvent evaporation. A 50 μl volume was injected on-column into GC-FID to achieve a detection limit for MOSH and MOAH below 1 mg/kg in most foods.
authors.affiliation: Official Food Control Authority of the Canton of Zürich, P.O. Box, CH-8032 Zürich, Switzerland.
authors.forename: Katell
authors.initials: K
authors.lastname: Fiselier
authors.forename: Florian
authors.initials: F
authors.lastname: Grundböck
authors.forename: Karsten
authors.initials: K
authors.lastname: Schön
authors.forename: Oliver
authors.initials: O
authors.lastname: Kappenstein
authors.forename: Karla
authors.initials: K
authors.lastname: Pfaff
authors.forename: Christoph
authors.initials: C
authors.lastname: Hutzler
authors.forename: Andreas
authors.initials: A
authors.lastname: Luch
authors.forename: Koni
authors.initials: K
authors.lastname: Grob
mesh_terms: D002849:Chromatography, Gas / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005504:Food Analysis / Q000379:methods*; D006586:Hexanes / Q000032:analysis / Q000737:chemistry; D006841:Hydrocarbons, Aromatic / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D008899:Mineral Oil / Q000032:analysis* / Q000737:chemistry; D010209:Paper; D015203:Reproducibility of Results; D058428:Silica Gel / Q000737:chemistry; D012835:Silver Nitrate / Q000737:chemistry; D014050:Toluene / Q000032:analysis / Q000737:chemistry; D014885:Waxes / Q000032:analysis / Q000737:chemistry
chemical_list: D006586:Hexanes; D006841:Hydrocarbons, Aromatic; D014885:Waxes; D014050:Toluene; D058428:Silica Gel; D008899:Mineral Oil; D012835:Silver Nitrate