title: [Simultaneous determination of eight iridoid glycosides in Gardeniae fructus by HPLC].
abstract: OBJECTIVE
  To establish an HPLC method for simultaneous determination of eight iridiods in Gardeniae Fructus.
  
  
  METHODS
  Kromasil C18 column (4. 6 mm x 250 mm, 5 microm) was adopted, with acetonitrile-water-trifluoroacetic acid (6:94: 0.05) as the mobile phase at the flow rate of 1.0 mL x min(-1). The detection wavelength was set at 238 nm, and the column temperature was 40 degrees C.
  
  
  RESULTS
  The linear ranges of geniposide, gardoside, shanzhiside, geniposidic acid, deacetyl asperulosidic acid methyl ester, gardenoside, scandoside methyl ester, and genipin gentiobioside were 1.5036 - 15.036, 0.04256 - 0.4256, 0.1038 - 1.038, 0.00992 - 0.0992, 0.02332 - 0.2332, 0.4128 - 4.128, 0.02040 - 0.2040 and 0.4656 - 4.656 microg, respectively. Their average recoveries were 99.6% , 100.6% , 101.2%, 99.5%, 100.3% , 98.7%, 99.8% and 100.1%, respectively.
  
  
  CONCLUSIONS
  The method shows good separation and it is so simple, accurate and highly repeatable that it can be used for providing basis for quality control of Gardeniae Fructus.
authors.affiliation: Lunan Pharmaceutical Group Co., Ltd., Linyi 276006, China. liuwuzhan@163.com
authors.forename: Wuzhan
authors.initials: W
authors.lastname: Liu
authors.forename: Jianwei
authors.initials: J
authors.lastname: Fan
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Gao
authors.forename: Xinjian
authors.initials: X
authors.lastname: Qiu
authors.forename: Huifang
authors.initials: H
authors.lastname: Zhuang
authors.forename: Zhiquan
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000302:isolation & purification; D032062:Gardenia / Q000737:chemistry*; D057889:Iridoid Glycosides / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D004365:Drugs, Chinese Herbal; D057889:Iridoid Glycosides