title: Successful cloning of coyotes through interspecies somatic cell nuclear transfer using domestic dog oocytes.
abstract: Interspecies somatic cell nuclear transfer (iSCNT) is an emerging assisted reproductive technology (ART) for preserving Nature's diversity. The scarcity of oocytes from some species makes utilisation of readily available oocytes inevitable. In the present study, we describe the successful cloning of coyotes (Canis latrans) through iSCNT using oocytes from domestic dogs (Canis lupus familiaris or dingo). Transfer of 320 interspecies-reconstructed embryos into 22 domestic dog recipients resulted in six pregnancies, from which eight viable offspring were delivered. Fusion rate and cloning efficiency during iSCNT cloning of coyotes were not significantly different from those observed during intraspecies cloning of domestic dogs. Using neonatal fibroblasts as donor cells significantly improved the cloning efficiency compared with cloning using adult fibroblast donor cells (P<0.05). The use of domestic dog oocytes in the cloning of coyotes in the present study holds promise for cloning other endangered species in the Canidae family using similar techniques. However, there are still limitations of the iSCNT technology, as demonstrated by births of morphologically abnormal coyotes and the clones' inheritance of maternal domestic dog mitochondrial DNA.
authors.affiliation: Sooam Biotech Research Foundation, 64 Kyungin-ro, Guro-gu, Seoul 152-895, Republic of Korea.
authors.forename: Insung
authors.initials: I
authors.lastname: Hwang
authors.forename: Yeon Woo
authors.initials: YW
authors.lastname: Jeong
authors.forename: Joung Joo
authors.initials: JJ
authors.lastname: Kim
authors.forename: Hyo Jeong
authors.initials: HJ
authors.lastname: Lee
authors.forename: Mina
authors.initials: M
authors.lastname: Kang
authors.forename: Kang Bae
authors.initials: KB
authors.lastname: Park
authors.forename: Jung Hwan
authors.initials: JH
authors.lastname: Park
authors.forename: Yeun Wook
authors.initials: YW
authors.lastname: Kim
authors.forename: Woo Tae
authors.initials: WT
authors.lastname: Kim
authors.forename: Taeyoung
authors.initials: T
authors.lastname: Shin
authors.forename: Sang Hwan
authors.initials: SH
authors.lastname: Hyun
authors.forename: Eui-Bae
authors.initials: EB
authors.lastname: Jeung
authors.forename: Woo Suk
authors.initials: WS
authors.lastname: Hwang
mesh_terms: D000818:Animals; D017849:Animals, Inbred Strains; D002478:Cells, Cultured; D019976:Cloning, Organism / Q000009:adverse effects / Q000662:veterinary*; D000013:Congenital Abnormalities / Q000209:etiology / Q000662:veterinary; D046029:Coyotes / Q000235:genetics* / Q000502:physiology; D003433:Crosses, Genetic; D004272:DNA, Mitochondrial / Q000378:metabolism; D004285:Dogs; D004624:Embryo Transfer / Q000662:veterinary; D056727:Endangered Species; D005260:Female; D005347:Fibroblasts / Q000502:physiology*; D050498:Live Birth / Q000662:veterinary; D008297:Male; D018895:Microsatellite Repeats; D053652:Nuclear Transfer Techniques / Q000009:adverse effects / Q000662:veterinary*; D054315:Oocyte Retrieval / Q000662:veterinary; D009865:Oocytes / Q000502:physiology*; D011247:Pregnancy; D056910:Republic of Korea; D050497:Stillbirth / Q000662:veterinary
chemical_list: D004272:DNA, Mitochondrial