title: Involvement of intestinal intraepithelial lymphocytes in turnover of intestinal epithelial cells: morphological and functional alterations due to daily administration of FK506.
abstract: To elucidate the interaction of intestinal intraepithelial lymphocytes (IELs) with intestinal epithelial cells (IECs), we investigated alterations of IECs by activating or inactivating IELs. The stimulation of IELs with anti-mouse CD3 monoclonal antibody induced massive apoptosis of IECs. Changes in IECs and IELs from mice that received daily administration of FK506 for 14days were investigated. IELs, particularly TCR-γδ⁺ IELs, were reduced in cell number, and a decrease of cytotoxic activity was observed. Under this condition, loss of apoptotic cells at the tips of villi and delayed turnover of IECs were detected. The expressions of alkaline phosphatase and CD98 amino acid transporters on IECs were decreased. Furthermore, abnormal skeletal organization of villi and weakened binding of IECs to the basement membrane were shown. These results suggest that inactivated IECs, which should be led to apoptosis, remained. It was strongly suggested that IELs participated in IEC turnover.
authors.affiliation: Cell Biology Laboratory, Department of Pharmaceutical Sciences, Faculty of Pharmacy, Kinki University, 3-4-1 Kowakae, Higashiosaka-shi, Osaka 577-8502, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Hidaka
authors.forename: Masanori
authors.initials: M
authors.lastname: Nakamura
authors.forename: Yukari
authors.initials: Y
authors.lastname: Ohmichi
authors.forename: Jun
authors.initials: J
authors.lastname: Itoh
authors.forename: Kenji
authors.initials: K
authors.lastname: Fukuzawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Masuko
authors.forename: Hideki
authors.initials: H
authors.lastname: Yagi
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D017209:Apoptosis; D017252:CD3 Complex / Q000276:immunology; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000187:drug effects / Q000276:immunology; D027261:Fusion Regulatory Protein-1 / Q000096:biosynthesis; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D007422:Intestines / Q000166:cytology* / Q000276:immunology; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology* / Q000378:metabolism; D016559:Tacrolimus / Q000494:pharmacology*
chemical_list: D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D027261:Fusion Regulatory Protein-1; D007166:Immunosuppressive Agents; D016692:Receptors, Antigen, T-Cell, gamma-delta; D000469:Alkaline Phosphatase; D016559:Tacrolimus