title: [Study on HPLC fingerprint of Oldenlandia diffusa].
abstract: OBJECTIVE
  To establish the HPLC fingerprint chromatogram of Oldenlandia diffusa coupled with chemometrics means for the quality control of multi-batches of medicinal material.
  
  
  METHODS
  The separation was developed on C18 column(4.6 mm x 250 mm, 5 microm) by gradient elution with acetonitrile-water(both containing 0.1 per thousand (V/V) ocetic acid) as mobile phase at a flow rate of 0.8 mL/min, the detection wavelength at 238 nm and column temperature at 30 degrees C. The HPLC fingerprint chromatogram of Oldenlandia diffusa was set up and the main characteristic peaks were identified by comparing with chemical reference substance. The quality of 22 batches of medicinal material was evaluated by similarity assay as well as principal component analysis (PCA) and cluster analysis.
  
  
  RESULTS
  The established HPLC fingerprint chromatogram of Oldenlandia diffusa was specific, precise, reproducible and stable. 11 peaks were chemically identified. The similarity of 17 batches of Oldenlandia diffusa was obviously higher than 5 batches of adulterants. PCA showed that 17 batches of Oldenlandia diffusa were in a domain and 5 batches of adulterants were far apart from the domain. The cluster analysis of the 22 batches of medicinal material showed that 17 batches of Oldenlandia diffusa were in a cluster while 5 batches of adulterants were excluded. Further cluster analysis was carried out for the quality consistency of 17 batches of Oldenlandia diffusa and accordingly they were devided into 4 clusters.
  
  
  CONCLUSIONS
  With the combination of chemometrics means, the HPLC fingerprint chromatogram provides a method for evaluation of authenticity and quality control of Oldenlandia diffusa, which is favorable to improve overall quality control of Oldenlandia diffusa.
authors.affiliation: Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, China. jxzscy@163.com
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Yao
authors.forename: Yi
authors.initials: Y
authors.lastname: Dai
authors.forename: Hong
authors.initials: H
authors.lastname: Cheng
authors.forename: Li-Rong
authors.initials: LR
authors.lastname: Wen
authors.forename: Guang-Xiong
authors.initials: GX
authors.lastname: Zhou
authors.forename: Xin-Sheng
authors.initials: XS
authors.lastname: Yao
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016000:Cluster Analysis; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D039823:Iridoids / Q000032:analysis; D032067:Oldenlandia / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D025341:Principal Component Analysis; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D004365:Drugs, Chinese Herbal; D039823:Iridoids