title: Centrosomal MPF triggers the mitotic and morphogenetic switches of fission yeast.
abstract: Activation of mitosis-promoting factor (MPF) drives mitotic commitment. In human cells active MPF appears first on centrosomes. We show that local activation of MPF on the equivalent organelle of fission yeast, the spindle pole body (SPB), promotes Polo kinase activity at the SPBs long before global MPF activation drives mitotic commitment. Artificially promoting MPF or Polo activity at various locations revealed that this local control of Plo1 activity on G2 phase SPBs dictates the timing of mitotic commitment. Cytokinesis of the rod-shaped fission yeast cell generates a naive, new, cell end. Growth is restricted to the experienced old end until a point in G2 phase called new end take off (NETO) when bipolar growth is triggered. NETO coincided with MPF activation of Plo1 on G2 phase SPBs (ref. 4). Both MPF and Polo activities were required for NETO and both induced NETO when ectopically activated at interphase SPBs. NETO promotion by MPF required polo. Thus, local MPF activation on G2 SPBs directs polo kinase to control at least two distinct and temporally separated, cell-cycle transitions at remote locations.
authors.affiliation: CRUK Cell Division Group, Paterson Institute for Cancer Research, Wilmslow Road, Manchester, M20 4BX, UK.
authors.forename: Agnes
authors.initials: A
authors.lastname: Grallert
authors.forename: Avinash
authors.initials: A
authors.lastname: Patel
authors.forename: Victor A
authors.initials: VA
authors.lastname: Tallada
authors.forename: Kuan Yoow
authors.initials: KY
authors.lastname: Chan
authors.forename: Steven
authors.initials: S
authors.lastname: Bagley
authors.forename: Andrea
authors.initials: A
authors.lastname: Krapp
authors.forename: Viesturs
authors.initials: V
authors.lastname: Simanis
authors.forename: Iain M
authors.initials: IM
authors.lastname: Hagan
mesh_terms: D018385:Centrosome; D004789:Enzyme Activation; D004795:Enzyme Stability; D025461:Feedback, Physiological; D016195:G2 Phase; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006207:Half-Life; D016200:Maturation-Promoting Factor / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008938:Mitosis; D008954:Models, Biological; D009024:Morphogenesis; D010750:Phosphoproteins / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012568:Schizosaccharomyces / Q000254:growth & development / Q000502:physiology* / Q000648:ultrastructure; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism; D059008:Time-Lapse Imaging
chemical_list: C111708:Cut12 protein, S pombe; D008869:Microtubule-Associated Proteins; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; D029702:Schizosaccharomyces pombe Proteins; D049452:Green Fluorescent Proteins; C501407:Plo1 protein, S pombe; D017346:Protein Serine-Threonine Kinases; D016200:Maturation-Promoting Factor