title: Genotoxicity evaluation of dental restoration nanocomposite using comet assay and chromosome aberration test.
abstract: Nanocomposite is used as a dental filling to restore the affected tooth, especially in dental caries. The dental nanocomposite (KelFil) for tooth restoration used in this study was produced by the School of Dental Sciences, Universiti Sains Malaysia, Malaysia and is incorporated with monodispersed, spherical nanosilica fillers. The aim of the study was to determine the genotoxic effect of KelFil using in vitro genotoxicity tests. The cytotoxicity and genotoxicity of KelFil was evaluated using MTT assay, comet assay and chromosome aberration tests with or without the addition of a metabolic activation system (S9 mix), using the human lung fibroblast cell line (MRC-5). Concurrent negative and positive controls were included. In the comet assay, no comet formation was found in the KelFil groups. There was a significant difference in tail moment between KelFil groups and positive control (p < 0.05). Similarly, no significant aberrations in chromosomes were noticed in KelFil groups. The mitotic indices of treatment groups and negative control were significantly different from positive controls. Hence, it can be concluded that the locally produced dental restoration nanocomposite (KelFil) is non-genotoxic under the present test conditions.
authors.affiliation: School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
authors.forename: Marahaini
authors.initials: M
authors.lastname: Musa
authors.forename: Kannan Thirumulu
authors.initials: KT
authors.lastname: Ponnuraj
authors.forename: Dasmawati
authors.initials: D
authors.lastname: Mohamad
authors.forename: Ismail Ab
authors.initials: IA
authors.lastname: Rahman
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002869:Chromosome Aberrations; D020552:Comet Assay / Q000379:methods*; D017266:Dental Prosthesis; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008940:Mitotic Index; D009152:Mutagenicity Tests / Q000379:methods*; D053761:Nanocomposites / Q000633:toxicity*