title: A novel thermotolerant Pediococcus acidilactici B-25 strain for color, COD, and BOD reduction of distillery effluent for end use applications.
abstract: The present study was aimed to characterize physico-chemical and microbial population of distillery effluent and isolate a novel thermotolerant bacterium for color, COD, and BOD reduction of spentwash. The level of alkalinity, TSS, DO, COD, BOD, TN, ammonical nitrogen, nitrate nitrogen, phosphorous, potassium, chloride, and calcium of spentwash (SW), bioreactor effluent (BE), and secondary treated effluent (STE) were well above the permissible limits. The level of color, TS, and TDS were under the permissible limits for STE but not for SW and BE. The microbial population was higher in BE. The results revealed that effluent was highly polluted and require suitable treatment before discharge. A novel thermotolerant bacterium, identified as Pediococcus acidilactici, was isolated which exhibited maximum 79 % decolorization, 85 % COD, and 94 % BOD reduction at 45 °C using 0.1 %, glucose; 0.1 %, peptone; 0.05 %, MgSO4; 0.05 %, K2HPO4; pH 6.0 within 24 h under static condition. The ability of this strain to decolorize melanoidin at minimum carbon and nitrogen supplementation warrants its possible application for effluent treatment at industrial level. In addition, it is first instance when melanoidin decolorization was reported by P. acidilactici. This study could be an approach towards control of environmental pollution and health hazards of people in and around the effluent distillery unit.
authors.affiliation: Department of Microbiology (Centre of Excellence), Dr. Ram Manohar Lohia Avadh University, Faizabad, 224001, Uttar Pradesh, India.
authors.forename: Soni
authors.initials: S
authors.lastname: Tiwari
authors.forename: Priyanka
authors.initials: P
authors.lastname: Rai
authors.forename: Santosh Kumar
authors.initials: SK
authors.lastname: Yadav
authors.forename: Rajeeva
authors.initials: R
authors.lastname: Gaur
mesh_terms: D001673:Biodegradation, Environmental; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors; D055598:Chemical Phenomena; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000737:chemistry; D010376:Pediococcus / Q000145:classification* / Q000302:isolation & purification*; D011108:Polymers / Q000378:metabolism; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000032:analysis / Q000382:microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis*; D018508:Water Purification / Q000379:methods
chemical_list: D009584:Nitrogen; D011108:Polymers; D062065:Wastewater; D014874:Water Pollutants, Chemical; C011908:melanoidin polymers