title: [DNA sequence analysis of Jk(a-b-) phenotype of blood donors from Chengdu].
abstract: OBJECTIVE
  To study the molecular genetics characteristics of Jk(a-b-) phenotype of blood donors from Chengdu.
  
  
  METHODS
  Exons 4-11 of the JK genes and their flanking intronic regions for 8 Jk(a-b-) samples were analyzed with PCR-sequence specific primers (PCR-SSP) and DNA sequencing.
  
  
  RESULTS
  All samples had AA genotype at position 838 of exon 9 predicting a null Jk(b)-like alleles. Sequence analysis has revealed 4 mutant alleles, which included: (1) IVS5-1G>A, A to G at position 588 (Pro196Pro) of exon 7; (2) G to A at position 896 (Gly299Glu) of exon 9, A to G at position 588 (Pro196Pro) of exon 7; (3) IVS5-1G>A, C>A at position 222 (Asn74Lys) of exon 5, A to G at position 499 (Met167Val) of exon 7, A to G at position 588 (Pro196Pro) of exon 7; and (4) IVS5-1G>A, G to A at position 896 (Gly299Glu) of exon 9, A to G at position 588 (Pro196Pro) of exon 7.
  
  
  CONCLUSIONS
  IVS5-1G>A, C to A at position 222 (Asn74Lys) of exon 5 and G to A at position 896 (Gly299Glu) of exon 9 might have been the molecular genetic mechanisms underlying Jk(a-b-) phenotype of the selected blood donors.
authors.affiliation: Blood Research Laboratory, Chengdu Blood Center, Chengdu, Sichuan, 610041 P R China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Hong
authors.forename: Tian-xiang
authors.initials: TX
authors.lastname: Gong
authors.forename: Chang-hua
authors.initials: CH
authors.lastname: Zhou
mesh_terms: D000483:Alleles; D001483:Base Sequence; D001782:Blood Donors; D002681:China; D005091:Exons; D005838:Genotype; D006801:Humans; D007438:Introns; D007667:Kidd Blood-Group System / Q000235:genetics*; D009154:Mutation; D010641:Phenotype
chemical_list: D007667:Kidd Blood-Group System