title: An ethanol extract derived from Bonnemaisonia hamifera scavenges ultraviolet B (UVB) radiation-induced reactive oxygen species and attenuates UVB-induced cell damage in human keratinocytes.
abstract: The present study investigated the photoprotective properties of an ethanol extract derived from the red alga Bonnemaisonia hamifera against ultraviolet B (UVB)-induced cell damage in human HaCaT keratinocytes. The Bonnemaisonia hamifera ethanol extract (BHE) scavenged the superoxide anion generated by the xanthine/xanthine oxidase system and the hydroxyl radical generated by the Fenton reaction (FeSO₄ + H₂O₂), both of which were detected by using electron spin resonance spectrometry. In addition, BHE exhibited scavenging activity against the 1,1-diphenyl-2-picrylhydrazyl radical and intracellular reactive oxygen species (ROS) that were induced by either hydrogen peroxide or UVB radiation. BHE reduced UVB-induced apoptosis, as shown by decreased apoptotic body formation and DNA fragmentation. BHE also attenuated DNA damage and the elevated levels of 8-isoprostane and protein carbonyls resulting from UVB-mediated oxidative stress. Furthermore, BHE absorbed electromagnetic radiation in the UVB range (280-320 nm). These results suggest that BHE protects human HaCaT keratinocytes against UVB-induced oxidative damage by scavenging ROS and absorbing UVB photons, thereby reducing injury to cellular components.
authors.affiliation: School of Medicine, Jeju National University, Jeju 690-756, Korea.
authors.forename: Mei Jing
authors.initials: MJ
authors.lastname: Piao
authors.forename: Yu Jae
authors.initials: YJ
authors.lastname: Hyun
authors.forename: Suk Ju
authors.initials: SJ
authors.lastname: Cho
authors.forename: Hee Kyoung
authors.initials: HK
authors.lastname: Kang
authors.forename: Eun Sook
authors.initials: ES
authors.lastname: Yoo
authors.forename: Young Sang
authors.initials: YS
authors.lastname: Koh
authors.forename: Nam Ho
authors.initials: NH
authors.lastname: Lee
authors.forename: Mi Hee
authors.initials: MH
authors.lastname: Ko
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D053938:DNA Fragmentation / Q000187:drug effects / Q000528:radiation effects; D004578:Electron Spin Resonance Spectroscopy; D000431:Ethanol / Q000737:chemistry; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D015603:Keratinocytes / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D000461:Rhodophyta / Q000737:chemistry*; D013481:Superoxides / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D013481:Superoxides; D000431:Ethanol; D006861:Hydrogen Peroxide