title: Evaluation of control mechanisms for Saccharomyces cerevisiae central metabolic reactions using metabolome data of eight single-gene deletion mutants.
abstract: We performed metabolome and metabolite-metabolite correlation analyses for eight single-gene deletion mutants of Saccharomyces cerevisiae to evaluate the physiology of glucose metabolism. The irreversible enzyme reactions can become bottlenecks when intracellular metabolism is perturbed by direct interference from the central metabolic pathway by gene deletions or by a deletion of transcriptional regulator. Metabolome data reveal that transcriptional factor, gcr2, regulates the reaction that converts 3-phosphoglycerate into phosphoenolpyruvate. Metabolome data also suggest that the reaction catalyzed by pyruvate kinase makes one of the rate-limiting reactions throughout the glycolytic pathway.
authors.affiliation: Biomass Engineering Program, RIKEN, 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Shirai
authors.forename: Fumio
authors.initials: F
authors.lastname: Matsuda
authors.forename: Mami
authors.initials: M
authors.lastname: Okamoto
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism; D005988:Glyceric Acids / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D055442:Metabolome; D010728:Phosphoenolpyruvate / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: C076228:GCR2 protein, S cerevisiae; D005988:Glyceric Acids; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D010728:Phosphoenolpyruvate; C005156:3-phosphoglycerate; D005947:Glucose