title: [Determination of probucol in human plasma by HPLC-MS/MS].
abstract: OBJECTIVE
  To develop a specific and sensitive method for the determination of probucol in human plasma using HPLC-MS/MS technique.
  
  
  METHODS
  Probucol were extracted from plasma by ethyl ether: dichloromethane (1:1, V/V), with physcion as an internal standard. The analytes went through the column of ultimate CN (50 mm x 4.6 mm, 5 microm) with mobile phase acetonitrile: water: ammonia water = 97:3:0.05 (adjusted pH = 7.2 with formic acid). Probucol was analyzed with a negative mode. The ion pairs being detected were 515.5-->236.1 (probucol) and 283.0-->239.9 physcion, respectively.
  
  
  RESULTS
  The established method was able to determine probucol in human plasma over the range of 2.5-6000 ng/mL, with a method recovery ranging from 93.02% to 104.12%. The intra and inter day variances were below 4.67% and 5.72%.
  
  
  CONCLUSIONS
  The HPLC-MS/MS method for analyzing probucol was validated. It is sensitive and suitable for pharmacokinetic studies of probucol.
authors.affiliation: Institute of Clinical Pharmacology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Xie
authors.forename: Jin
authors.initials: J
authors.lastname: Xiang
authors.forename: Qin
authors.initials: Q
authors.lastname: Yu
authors.forename: Mao-Zhi
authors.initials: MZ
authors.lastname: Liang
authors.forename: Feng
authors.initials: F
authors.lastname: Nan
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Qin
mesh_terms: D000924:Anticholesteremic Agents / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D011341:Probucol / Q000097:blood*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000924:Anticholesteremic Agents; D011341:Probucol