title: The metabolomic profile of umbilical cord blood in neonatal hypoxic ischaemic encephalopathy.
abstract: BACKGROUND
  Hypoxic ischaemic encephalopathy (HIE) in newborns can cause significant long-term neurological disability. The insult is a complex injury characterised by energy failure and disruption of cellular homeostasis, leading to mitochondrial damage. The importance of individual metabolic pathways, and their interaction in the disease process is not fully understood. The aim of this study was to describe and quantify the metabolomic profile of umbilical cord blood samples in a carefully defined population of full-term infants with HIE.
  
  
  RESULTS
  The injury severity was defined using both the modified Sarnat score and continuous multichannel electroencephalogram. Using these classification systems, our population was divided into those with confirmed HIE (n = 31), asphyxiated infants without encephalopathy (n = 40) and matched controls (n = 71). All had umbilical cord blood drawn and biobanked at -80 °C within 3 hours of delivery. A combined direct injection and LC-MS/MS assay (AbsolutIDQ p180 kit, Biocrates Life Sciences AG, Innsbruck, Austria) was used for the metabolomic analyses of the samples. Targeted metabolomic analysis showed a significant alteration between study groups in 29 metabolites from 3 distinct classes (Amino Acids, Acylcarnitines, and Glycerophospholipids). 9 of these metabolites were only significantly altered between neonates with Hypoxic ischaemic encephalopathy and matched controls, while 14 were significantly altered in both study groups. Multivariate Discriminant Analysis models developed showed clear multifactorial metabolite associations with both asphyxia and HIE. A logistic regression model using 5 metabolites clearly delineates severity of asphyxia and classifies HIE infants with AUC = 0.92. These data describe wide-spread disruption to not only energy pathways, but also nitrogen and lipid metabolism in both asphyxia and HIE.
  
  
  CONCLUSIONS
  This study shows that a multi-platform targeted approach to metabolomic analyses using accurately phenotyped and meticulously biobanked samples provides insight into the pathogenesis of perinatal asphyxia. It highlights the potential for metabolomic technology to develop a diagnostic test for HIE.
authors.affiliation: Neonatal Brain Research Group, Department of Paediatrics and Child Health, Cork University Maternity Hospital, Wilton, Cork, Ireland. Bh.walsh@ucc.ie
authors.forename: Brian H
authors.initials: BH
authors.lastname: Walsh
authors.forename: David I
authors.initials: DI
authors.lastname: Broadhurst
authors.forename: Rupasri
authors.initials: R
authors.lastname: Mandal
authors.forename: David S
authors.initials: DS
authors.lastname: Wishart
authors.forename: Geraldine B
authors.initials: GB
authors.lastname: Boylan
authors.forename: Louise C
authors.initials: LC
authors.lastname: Kenny
authors.forename: Deirdre M
authors.initials: DM
authors.lastname: Murray
mesh_terms: D016022:Case-Control Studies; D002853:Chromatography, Liquid; D004569:Electroencephalography; D005260:Female; D005312:Fetal Blood / Q000378:metabolism*; D006801:Humans; D020925:Hypoxia-Ischemia, Brain / Q000097:blood* / Q000503:physiopathology; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D055432:Metabolomics; D053719:Tandem Mass Spectrometry