title: Excretions/secretions from bacteria-pretreated maggot are more effective against Pseudomonas aeruginosa biofilms.
abstract: BACKGROUND
  Sterile larvae--maggots of the green bottle blowfly Lucilia sericata are employed as a treatment tool for various types of chronic wounds. Previous studies reported that excretions/secretions (ES) of the sterile larvae could prevent and remove the biofilms of various species of bacteria. In the present study we assessed the effect of ES from the larvae pretreated with Pseudomonas aeruginosa on the bacteria biofilms.
  
  
  RESULTS
  We investigated the effects of ES from the maggot pretreated with P. aeruginosa on the biofilms using microtitre plate assays and on bactericidal effect using the colony-forming unit (CFU) assay. The results showed that only 30 µg of the ES from the pretreated maggots could prevent and degrade the biofilm of P. aeruginosa. However, the CFU count of P. aeruginosa was not decrease when compared to the ES from non pretreated maggots in this study condition. It is suggested that the ES from the pretreated maggot was more effective against biofilm of P. aeruginosa than sterile maggot ES.
  
  
  CONCLUSIONS
  Our results showed that the maggot ES, especially the bacteria-pretreated larva ES may provide a new insight into the treatment tool of the bacterial biofilms.
authors.affiliation: Department of Endocrinology, 454 Hospital of PLA, Nanjing, Jiangsu Province, China.
authors.forename: Ke-chun
authors.initials: KC
authors.lastname: Jiang
authors.forename: Xin-juan
authors.initials: XJ
authors.lastname: Sun
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Lan
authors.initials: L
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Cai
authors.forename: Yin-chen
authors.initials: YC
authors.lastname: Chen
authors.forename: Ning
authors.initials: N
authors.lastname: Luo
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Yu
authors.forename: Da-yong
authors.initials: DY
authors.lastname: Cai
authors.forename: Ai-ping
authors.initials: AP
authors.lastname: Wang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D012634:Bodily Secretions / Q000737:chemistry*; D004175:Diptera / Q000737:chemistry* / Q000276:immunology / Q000382:microbiology; D007814:Larva / Q000737:chemistry* / Q000276:immunology / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents