title: Neuropathogenesis of a mouse-adapted porcine epidemic diarrhea virus infection in suckling mice.
abstract: A mouse-adapted porcine epidemic diarrhea virus, MK-p10, showed higher neurovirulence in suckling mice than a non-adapted MK strain. There was no difference in virus growth, whereas clear differences between these two virus infections existed in the type of target cells infected, the spread of virus and the cytokine levels produced in the brain. In the early phase of infection, neurons, astrocytes and neural progenitor cells were infected by MK-p10, whereas neural progenitor cells were the only target cells infected by MK. On days 4-5 post-inoculation, MK-p10 antigens were distributed in a number of neurons in a wide area of the brain; however, antigens were restricted in MK infection. In moribund mice in both infection groups, viral antigens were found in a wide area of the brain. The wide spectrum of initial target cells following MK-p10 infection, as well as its faster spread in the brain, may be evidence of enhanced virulence in suckling mice.
authors.affiliation: Department of Veterinary Pathology, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, Musashino, Tokyo, Japan.|Department of Pathology, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan.|Department of Virology and Viral Infections, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, Musashino, Tokyo, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Kotani
authors.affiliation: Departments of Virology III, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Shirato
authors.affiliation: Department of Pathology, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan.
authors.forename: Noriyo
authors.initials: N
authors.lastname: Nagata
authors.affiliation: Department of Veterinary Public Health, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, Musashino, Tokyo, Japan.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Ikeda
authors.affiliation: Department of Veterinary Pathology, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, Musashino, Tokyo, Japan.
authors.forename: Kimimasa
authors.initials: K
authors.lastname: Takahashi
authors.affiliation: Department of Virology and Viral Infections, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, Musashino, Tokyo, Japan.
authors.forename: Fumihiro
authors.initials: F
authors.lastname: Taguchi
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000821:virology; D001921:Brain / Q000473:pathology / Q000821:virology; D051379:Mice; D009422:Nervous System Diseases / Q000473:pathology / Q000821:virology*; D058953:Neural Stem Cells / Q000821:virology; D009474:Neurons / Q000821:virology; D053485:Porcine epidemic diarrhea virus / Q000472:pathogenicity*; D014774:Virulence