title: Fast multicolor 3D imaging using aberration-corrected multifocus microscopy.
abstract: Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image.
authors.affiliation: Joint Graduate Group in Bioengineering, University of California, San Francisco/University of California, Berkeley, San Francisco, California, USA. sara.abrahamsson@gmail.com
authors.forename: Sara
authors.initials: S
authors.lastname: Abrahamsson
authors.forename: Jiji
authors.initials: J
authors.lastname: Chen
authors.forename: Bassam
authors.initials: B
authors.lastname: Hajj
authors.forename: Sjoerd
authors.initials: S
authors.lastname: Stallinga
authors.forename: Alexander Y
authors.initials: AY
authors.lastname: Katsov
authors.forename: Jan
authors.initials: J
authors.lastname: Wisniewski
authors.forename: Gaku
authors.initials: G
authors.lastname: Mizuguchi
authors.forename: Pierre
authors.initials: P
authors.lastname: Soule
authors.forename: Florian
authors.initials: F
authors.lastname: Mueller
authors.forename: Claire
authors.initials: C
authors.lastname: Dugast Darzacq
authors.forename: Xavier
authors.initials: X
authors.lastname: Darzacq
authors.forename: Carl
authors.initials: C
authors.lastname: Wu
authors.forename: Cornelia I
authors.initials: CI
authors.lastname: Bargmann
authors.forename: David A
authors.initials: DA
authors.lastname: Agard
authors.forename: Maxime
authors.initials: M
authors.lastname: Dahan
authors.forename: Mats G L
authors.initials: MG
authors.lastname: Gustafsson
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000201:enzymology; D017173:Caenorhabditis elegans / Q000166:cytology*; D058948:Cell Tracking; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000166:cytology*; D012516:Osteosarcoma / Q000201:enzymology; D012319:RNA Polymerase II / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
chemical_list: C092746:CSE4 protein, S cerevisiae; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D012319:RNA Polymerase II