title: [Callus cultivation and determination of gentiopicroside from Gentiana macrophylla].
abstract: OBJECTIVE
  To explore and improve the utilization efficiency of Gentiana macrophylla resources.
  
  
  METHODS
  The germination of Gentiona macrophylla seeds were used as explants to induce calluses; Cultured on various culture media and condition for callus induction and subculture by test; The content of gentiopicroside in callus was determined by HPLC.
  
  
  RESULTS
  The optimum medium for callus was MS +2,4-D (1.0 mg/L) + 6-BA (0.5 mg/L), and MS +2,4-D (1.0 mg/L) +6-BA (0.5 mg/L) + NAA (0.5 mg/L) was the optimum for subculture,the optimum time of subculture was 30 d; The accumulated gentiopicroside reached the highest level with 0.78 mg/g DW when entered 28-30 d.
  
  
  CONCLUSIONS
  The optimized culture method of callus of Gentiana. macrophylla is obtained; The growth curve of callus of Gentiona macrophylla turns on "S" appearance and the accumulated gentiopicroside increases along with the callus appreciation.
authors.affiliation: Department of Biology, Gansu College of TCM, Lanzhou 730000, China. swjys@gszy.edu.cn
authors.forename: Qian
authors.initials: Q
authors.lastname: Hou
authors.forename: Mei
authors.initials: M
authors.lastname: Guo
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.forename: Jin-xia
authors.initials: JX
authors.lastname: Zhou
authors.forename: Li-sha
authors.initials: LS
authors.lastname: Liu
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003247:Conservation of Natural Resources; D003470:Culture Media; D030012:Gentiana / Q000254:growth & development* / Q000378:metabolism; D018525:Germination; D057888:Iridoid Glucosides / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000494:pharmacology; D010946:Plants, Medicinal / Q000254:growth & development* / Q000378:metabolism; D036226:Seedlings / Q000254:growth & development; D012639:Seeds / Q000254:growth & development / Q000378:metabolism; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D003470:Culture Media; D057888:Iridoid Glucosides; D010937:Plant Growth Regulators; C012997:gentiopicroside; D015084:2,4-Dichlorophenoxyacetic Acid