title: Genetic diversity and investigation of polledness in divergent goat populations using 52 088 SNPs.
abstract: The recent availability of a genome-wide SNP array for the goat genome dramatically increases the power to investigate aspects of genetic diversity and to conduct genome-wide association studies in this important domestic species. We collected and analysed genotypes from 52 088 SNPs in Boer, Cashmere and Rangeland goats that had both polled and horned individuals. Principal components analysis revealed a clear genetic division between animals for each population, and model-based clustering successfully detected evidence of admixture that matched aspects of their recorded history. For example, shared co-ancestry was detected, suggesting Boer goats have been introgressed into the Rangeland population. Further, allele frequency data successfully tracked the altered genetic profile that has taken place after 40 years of breeding Australian Cashmere goats using the Rangeland animals as the founding population. Genome-wide association mapping of the POLL locus revealed a strong signal on goat chromosome 1. The 769-kb critical interval contained the polled intersex syndrome locus, confirming the genetic basis in non-European animals is the same as identified previously in Saanen goats. Interestingly, analysis of the haplotypes carried by a small set of sex-reversed animals, known to be associated with polledness, revealed some animals carried the wild-type chromosome associated with the presence of horns. This suggests a more complex basis for the relationship between polledness and the intersex condition than initially thought while validating the application of the goat SNP50 BeadChip for fine-mapping traits in goat.
authors.affiliation: CSIRO, Brisbane, Qld, Australia. James.Kijas@csiro.au
authors.forename: James W
authors.initials: JW
authors.lastname: Kijas
authors.forename: Judit S
authors.initials: JS
authors.lastname: Ortiz
authors.forename: Russell
authors.initials: R
authors.lastname: McCulloch
authors.forename: Andrew
authors.initials: A
authors.lastname: James
authors.forename: Blair
authors.initials: B
authors.lastname: Brice
authors.forename: Ben
authors.initials: B
authors.lastname: Swain
authors.forename: Gwenola
authors.initials: G
authors.lastname: Tosser-Klopp
mesh_terms: D000483:Alleles; D000818:Animals; D001315:Australia; D001947:Breeding; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies / Q000379:methods; D056426:Genetic Loci; D005828:Genetics, Population; D006041:Goats / Q000235:genetics*; D006239:Haplotypes; D006733:Horns; D008297:Male; D020641:Polymorphism, Single Nucleotide