title: QTL involved in the modification of cyanidin compounds in black and red raspberry fruit.
abstract: Fruit from Rubus species are highly valued for their flavor and nutritive qualities. Anthocyanin content contributes to these qualities, and although many studies have been conducted to identify and quantify the major anthocyanin compounds from various Rubus species, the genetic control of the accumulation of these complex traits in Rubus is not yet well understood. The identification of the regions of the genome involved in the production of anthocyanins is an important first step in identifying the genes underlying their expression. In this study, ultra and high-performance liquid chromatography (UHPLC and HPLC) and two newly developed Rubus linkage maps were used to conduct QTL analyses to explore the presence of associations between concentrations of five anthocyanins in fruit and genotype. In total, 27 QTL were identified on the Rubus linkage maps, four of which are associated with molecular markers designed from transcription factors and three of which are associated with molecular markers designed from anthocyanin biosynthetic pathway candidate genes. The results of this study suggest that, while QTL for anthocyanin accumulation have been identified on six of seven Rubus linkage groups (RLG), the QTL on RLG2 and RLG7 may be very important for genetic control of cyanidin modification in Rubus.
authors.affiliation: The New Zealand Institute for Plant & Food Research Limited (PFR), Batchelar Road, Private Bag 11600, Palmerston North, 4442, New Zealand. Jill.Bushakra@ars.usda.gov
authors.forename: J M
authors.initials: JM
authors.lastname: Bushakra
authors.forename: C
authors.initials: C
authors.lastname: Krieger
authors.forename: D
authors.initials: D
authors.lastname: Deng
authors.forename: M J
authors.initials: MJ
authors.lastname: Stephens
authors.forename: A C
authors.initials: AC
authors.lastname: Allan
authors.forename: R
authors.initials: R
authors.lastname: Storey
authors.forename: V V
authors.initials: VV
authors.lastname: Symonds
authors.forename: D
authors.initials: D
authors.lastname: Stevenson
authors.forename: T
authors.initials: T
authors.lastname: McGhie
authors.forename: D
authors.initials: D
authors.lastname: Chagné
authors.forename: E J
authors.initials: EJ
authors.lastname: Buck
authors.forename: S E
authors.initials: SE
authors.lastname: Gardiner
mesh_terms: D000872:Anthocyanins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002874:Chromosome Mapping; D004843:Epistasis, Genetic; D005638:Fruit / Q000737:chemistry / Q000235:genetics*; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D010641:Phenotype; D040641:Quantitative Trait Loci; D027824:Rosaceae / Q000737:chemistry / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D005819:Genetic Markers; D014157:Transcription Factors; C017154:cyanidin