title: Preparation and in vitro evaluation of folate-receptor-targeted SPION-polymer micelle hybrids for MRI contrast enhancement in cancer imaging.
abstract: Polymer-SPION hybrids were investigated for receptor-mediated localization in tumour tissue. Superparamagnetic iron oxide nanoparticles (SPIONs) prepared by high-temperature decomposition of iron acetylacetonate were monodisperse (9.27 ± 3.37 nm), with high saturation magnetization of 76.8 emu g(-1). Amphiphilic copolymers prepared from methyl methacrylate and PEG methacrylate by atom transfer radical polymerization were conjugated with folic acid (for folate-receptor specificity). The folate-conjugated polymer had a low critical micellar concentration (0.4 mg l(-1)), indicating stability of the micellar formulation. SPION-polymeric micelle clusters were prepared by desolvation of the SPION dispersion/polymer solution in water. Magnetic resonance imaging of the formulation revealed very good contrast enhancement, with transverse (T(2)) relaxivity of 260.4 mM(-1) s(-1). The biological evaluation of the SPION micelles included cellular viability assay (MTT) and uptake in HeLa cells. These studies demonstrated the potential use of these nanoplatforms for imaging and targeting.
authors.affiliation: Centre for Polymer Science and Engineering, Indian Institute of Technology Delhi, New Delhi-110016, India.
authors.forename: Shveta
authors.initials: S
authors.lastname: Mahajan
authors.forename: Veena
authors.initials: V
authors.lastname: Koul
authors.forename: Veena
authors.initials: V
authors.lastname: Choudhary
authors.forename: Gauri
authors.initials: G
authors.lastname: Shishodia
authors.forename: Alok C
authors.initials: AC
authors.lastname: Bharti
mesh_terms: D016923:Cell Death / Q000187:drug effects; D003287:Contrast Media; D003911:Dextrans / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity / Q000648:ultrastructure; D003952:Diagnostic Imaging / Q000379:methods*; D004705:Endocytosis / Q000187:drug effects; D058975:Folate Receptor 1 / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry / Q000633:toxicity / Q000648:ultrastructure; D008823:Micelles; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D009369:Neoplasms / Q000175:diagnosis* / Q000473:pathology; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D014961:X-Ray Diffraction
chemical_list: D003287:Contrast Media; D003911:Dextrans; D058975:Folate Receptor 1; D058185:Magnetite Nanoparticles; D008823:Micelles; D011108:Polymers; D011721:Pyrenes; C030984:pyrene; C065507:ferumoxides