title: Current methods for detecting ethylene in plants.
abstract: BACKGROUND
  In view of ethylene's critical developmental and physiological roles the gaseous hormone remains an active research topic for plant biologists. Progress has been made to understand the ethylene biosynthesis pathway and the mechanisms of perception and action. Still numerous questions need to be answered and findings to be validated. Monitoring gas production will very often complete the picture of any ethylene research topic. Therefore the search for suitable ethylene measuring methods for various plant samples either in the field, greenhouses, laboratories or storage facilities is strongly motivated.
  
  
  METHODS
  This review presents an update of the current methods for ethylene monitoring in plants. It focuses on the three most-used methods - gas chromatography detection, electrochemical sensing and optical detection - and compares them in terms of sensitivity, selectivity, time response and price. Guidelines are provided for proper selection and application of the described sensor methodologies and some specific applications are illustrated of laser-based detector for monitoring ethylene given off by Arabidopsis thaliana upon various nutritional treatments.
  
  
  CONCLUSIONS
  Each method has its advantages and limitations. The choice for the suitable ethylene sensor needs careful consideration and is driven by the requirements for a specific application.
authors.affiliation: Department of Molecular and Laser Physics, Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, NL 6525 AJ Nijmegen, The Netherlands. s.cristescu@science.ru.nl
authors.forename: Simona M
authors.initials: SM
authors.lastname: Cristescu
authors.forename: Julien
authors.initials: J
authors.lastname: Mandon
authors.forename: Denis
authors.initials: D
authors.lastname: Arslanov
authors.forename: Jérôme
authors.initials: J
authors.lastname: De Pessemier
authors.forename: Christian
authors.initials: C
authors.lastname: Hermans
authors.forename: Frans J M
authors.initials: FJ
authors.lastname: Harren
mesh_terms: D017360:Arabidopsis / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002849:Chromatography, Gas; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D005030:Ethylenes / Q000032:analysis* / Q000096:biosynthesis; D007834:Lasers; D010937:Plant Growth Regulators / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors; D055549:Volatile Organic Compounds / Q000032:analysis
chemical_list: D005030:Ethylenes; D010937:Plant Growth Regulators; D055549:Volatile Organic Compounds; C036216:ethylene