title: Metabolic flux analysis of genetically engineered Saccharomyces cerevisiae that produces lactate under micro-aerobic conditions.
abstract: In the present study, to elucidate mechanisms of growth suppression in YIBO-pdc1/5Δ, we performed carbon metabolic flux analysis under micro-aerobic conditions. Our results indicate that growth suppression of YIBO-pdc1/5Δ is caused by decreased flux to the pentose phosphate pathway, which supplies ribose-5-phosphate, a precursor for histidine synthesis in Sacchar omyces cerevisiae. In addition, significant accumulation of pyruvate was observed in the continuous culture.
authors.affiliation: Toyota Central R and D Laboratories Inc., 41-1 Yokomichi, Nagakute, Aichi, 480-1192, Japan. nagamori@bio.eng.osaka-u.ac.jp
authors.forename: Eiji
authors.initials: E
authors.lastname: Nagamori
authors.forename: Kazunori
authors.initials: K
authors.lastname: Shimizu
authors.forename: Hideaki
authors.initials: H
authors.lastname: Fujita
authors.forename: Kenro
authors.initials: K
authors.lastname: Tokuhiro
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Ishida
authors.forename: Haruo
authors.initials: H
authors.lastname: Takahashi
mesh_terms: D000332:Aerobiosis / Q000235:genetics; D005818:Genetic Engineering; D019344:Lactic Acid / Q000096:biosynthesis*; D010427:Pentose Phosphate Pathway; D019289:Pyruvic Acid / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
chemical_list: D019344:Lactic Acid; D019289:Pyruvic Acid