title: Immunohistochemistry of cytokeratins 7, 8, 17, 18, and 19, and GLUT-1 aids differentiation of desmoplastic malignant mesothelioma from fibrous pleuritis.
abstract: It is difficult to distinguish desmoplastic malignant mesothelioma (DMM) from fibrous pleuritis (FP). We investigated the utility of immunohistochemistry as a way of differentiating between DMM and FP. We examined 11 DMMs and 46 FPs with the aid of antibodies against 18 cytokeratin (CK) subtypes, calponin, caldesmon, desmin, and GLUT-1. The best sensitivity and specificity cut-off values in the receiver operating characteristic curves (ROC) for CKs 7, 8, 17, 18, and 19, and GLUT-1 were each above 60%. When cases with either DMM or FP were partitioned by the staining score associated with the best sensitivity and specificity cut-off values in ROC, the incidence of a positive expression for CKs 7, 8, 17, 18, and 19, and GLUT-1 was significantly higher in DMM than in FP. In conclusion, immunohistochemistry for CKs 7, 8, 17, 18, and 19, and GLUT-1 may be useful, alongside histological characteristics, for separating DMM from FP.
authors.affiliation: Department of Pathology and Laboratory Medicine, National Defense Medical College, Tokorozawa. Japan.
authors.forename: Toshikatsu
authors.initials: T
authors.lastname: Horiuchi
authors.forename: Sho
authors.initials: S
authors.lastname: Ogata
authors.forename: Susumu
authors.initials: S
authors.lastname: Tominaga
authors.forename: Sadayuki
authors.initials: S
authors.lastname: Hiroi
authors.forename: Kunimitsu
authors.initials: K
authors.lastname: Kawahara
authors.forename: Akira
authors.initials: A
authors.lastname: Hebisawa
authors.forename: Isao
authors.initials: I
authors.lastname: Irei
authors.forename: Ichiro
authors.initials: I
authors.lastname: Ito
authors.forename: Toru
authors.initials: T
authors.lastname: Kameya
authors.forename: Tohru
authors.initials: T
authors.lastname: Tsujimura
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakano
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Nakanishi
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kawai
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism*; D003937:Diagnosis, Differential; D005260:Female; D051272:Glucose Transporter Type 1 / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D053537:Keratin-17 / Q000378:metabolism; D053538:Keratin-18 / Q000378:metabolism; D053539:Keratin-19 / Q000378:metabolism; D053552:Keratin-7 / Q000378:metabolism; D053533:Keratin-8 / Q000378:metabolism; D007633:Keratins / Q000378:metabolism*; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008654:Mesothelioma / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D000086002:Mesothelioma, Malignant; D008875:Middle Aged; D010998:Pleurisy / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D012189:Retrospective Studies
chemical_list: D015415:Biomarkers; D014408:Biomarkers, Tumor; D051272:Glucose Transporter Type 1; D053537:Keratin-17; D053538:Keratin-18; D053539:Keratin-19; D053552:Keratin-7; D053533:Keratin-8; D007633:Keratins