title: [Analysis on anti-vascular inflammatory mechanism in vitro of total flavones from Artemisia anomala].
abstract: OBJECTIVE
  To study the impact of total flavones from Artemisia anomala (TFAS) on activation of macrophages, cell oxidative stress, auto-nitration of CuZn-SOD, platelet aggregation and isolated vascular tension.
  
  
  METHODS
  LPS and IFN-gamma induced activation of macrophages and oxidative stress in rats; H2O2 and nitrite induced auto-nitration of CuZn-SOD; ADP, AA and collagen induced platelet aggregation in vitro in mice; PE stimulates isolated vascular tension; nitrite content of macrophages was measured by Griess assay; MTT assay and FRAP assay was applied for cell viability and total cell antioxidant capacity; auto-nitration of CuZn-SOD was measured by Western blot and colorimetric methods; platelet aggregation was detected by turbidimetry; and aorta ring relaxation was recorded by isolated vascular function experience devices for rats.
  
  
  RESULTS
  TFAS demonstrated dose dependence (25, 50, 100, 200 mg x L(-1)) on inhibiting induced macrophages NO production from generating, while increasing cell viability and total anti-oxidant capacity. Auto-nitration of CuZn-SOD was suppressed by TFAS in dose dependence (0.5, 5, 50 mg x L(-1)). TFAS showed an inhibitory effect on collagen-induced platelet aggregation at 50 mg x L(-1) and an endothelium-dependent relaxation effect on PE-induced vasoconstriction at 1 g x L(-1).
  
  
  CONCLUSIONS
  TFAS shows effect on anti-inflammation, anti-oxidation, anti-nitration, anti-platelet aggregation and vasodilatation in experiment in vitro, which may inhibit vascular inflammatory by regulating multiple target points. It is among material bases for promoting blood circulation and removing blood stasis.
authors.affiliation: Shanghai SUNDISE Chinese Medicine Co. Ltd., Shanghai 200051, China.
authors.forename: Yi-feng
authors.initials: YF
authors.lastname: Pan
authors.forename: Dan-dan
authors.initials: DD
authors.lastname: Zhang
authors.forename: Shuang
authors.initials: S
authors.lastname: Ling
authors.forename: Hong-ping
authors.initials: HP
authors.lastname: Zhang
authors.forename: Hua-Shi
authors.initials: HS
authors.lastname: Bian
authors.forename: Ka
authors.initials: K
authors.lastname: Bian
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001011:Aorta / Q000187:drug effects / Q000276:immunology* / Q000502:physiology; D019306:Artemisia / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D047309:Flavones / Q000008:administration & dosage*; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D014664:Vasodilation / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D047309:Flavones