title: [Study of the lung surfactant maturity by biochemical and biophysical analyses of gasral aspirates from newborn infants].
abstract: The aim of the present study was to estimate the lung surfactant maturity by analyses of gastral aspirates (GA) from prematurely born and full-term infants. A biochemical analysis of the protein and lipid content in GA from the children groups tested was made. By thin-layer chromatography the individual phospholipids components in GA samples from prematurely born and full-term children were detected. In addition, by using the pending drop method the surface characteristics (equilibrium, maximal and minimal surface tension values) were determined. Our results showed an increase in the phospholipid and the protein concentrations in GA during pregnancy progress as well as significant differences in the individual phospholipids profile of the aspirates from prematurely born and full-term children. In case of surface characteristics a highest difference was observed for the minimal surface tension values (gammamin, m/Nm); while in the full-term children a lower surface tension was determined, in the prematurely born children significant higher values were reached, which define gammamin as the most informative from the parameters studied. Our results could find application into the clinical practice for fast surfactant maturity diagnostics in prematurely born children regarding lifesaving therapy with exogenous surfactants administration.
authors.forename: M
authors.initials: M
authors.lastname: Bangyozova
authors.forename: A
authors.initials: A
authors.lastname: Jordanova
authors.forename: A
authors.initials: A
authors.lastname: Tsanova
authors.forename: J
authors.initials: J
authors.lastname: Doumanov
authors.forename: E
authors.initials: E
authors.lastname: Christova
authors.forename: Z
authors.initials: Z
authors.lastname: Lalchev
mesh_terms: D002855:Chromatography, Thin Layer; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature / Q000378:metabolism*; D008168:Lung / Q000737:chemistry / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis* / Q000378:metabolism; D011247:Pregnancy; D037661:Pulmonary Surfactant-Associated Proteins / Q000032:analysis* / Q000378:metabolism; D011663:Pulmonary Surfactants / Q000032:analysis* / Q000378:metabolism; D012127:Respiratory Distress Syndrome, Newborn / Q000175:diagnosis / Q000378:metabolism; D013500:Surface Tension
chemical_list: D010743:Phospholipids; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants