title: [Pharmacokinetics and bioavailabilities of geniposide in Beagle dogs after oral administration Xingnaojing].
abstract: OBJECTIVE
  To establish a method for determination of geniposide in Beagle dogs plasma by high performance liquid chromatography (HPLC), and study the pharmacokinetics and bioavailability of geniposide in Beagle dogs after oral administration Xingnaojing.
  
  
  METHODS
  To determine the geniposide in Beagle dogs plasma by HPLC after oral administration or intravenous injection Xingnaojing, and the pharmacokinetic parameters were calculated by the software of Kinetica.
  
  
  RESULTS
  The good linearity range of geniposide was 1.24 - 158.88 mg x L(-1). The main pharmacokinetic parameters after oral administration was as follows: Cmax (11.8 +/- 0.6) mg x L(-1), Tmax (52.0 +/- 4.5) min, AUC(1280.8 +/- 172.0) mg x min x L(-1), MRT(118.7 +/- 25.4) min, and these parameters after intravenous injection was follows: Cmax 107.4 +/- 6.3) mg x L(-1), AUC(7930.1 +/- 670.0) mg x min x L(-1), MRT(92.4 +/- 5.1) min. The bioavailability of geniposide in Beagle dogs after oral administration Xingnaojing was (6.46 +/- 0.87)%.
  
  
  CONCLUSIONS
  The HPLC method had good applicability. The extract recovery, method recovery, intra-day precision and inter-day precision of the method were all met the requirements. The stability in conditions of room temperature and freeze-thaw cycle was good. The results indicated that the oral administration bioavailability of geniposide was in low degree.
authors.affiliation: Beijing University of Chinese Medicine, Beiing 100102, China. 04xft@sina.com
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Tian
authors.forename: Pengyue
authors.initials: P
authors.lastname: Li
authors.forename: Hongjie
authors.initials: H
authors.lastname: Wang
authors.forename: Baolin
authors.initials: B
authors.lastname: Bian
authors.forename: Shouying
authors.initials: S
authors.lastname: Du
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D039823:Iridoids / Q000008:administration & dosage / Q000493:pharmacokinetics*
chemical_list: D004365:Drugs, Chinese Herbal; D039823:Iridoids; C007835:geniposide