title: Real-time phase-contrast imaging of photothermal treatment of head and neck squamous cell carcinoma: an in vitro study of macrophages as a vector for the delivery of gold nanoshells.
abstract: Photothermal treatment (PTT) using nanoparticles has gained attention as a promising alternative therapy for malignant tumors. One strategy for increasing the selectivity of PTT is the use of macrophages as a cellular vector for delivering nanoparticles. The aim of the present study is to examine the use of macrophages as a cellular vector for efficient PTT and determine the appropriate irradiation power and time of a near-infrared (NIR) laser using real-time phase-contrast imaging. Thermally induced injury and death of cancer cells were found to begin at 44°C to 45°C, which was achieved using the PTT effect with gold nanoshells (NS) and irradiation with a NIR laser at a power of 2 W for 5 min. The peritoneal macrophage efficiently functioned as a cellular vector for the NS, and the cancer cells surrounding the NS-loaded macrophages selectively lost their cellular viability after being irradiated with the NIR laser.
authors.affiliation: Korea University, Department of Physics, Seoul, Republic of Korea.
authors.forename: Taeseok Daniel
authors.initials: TD
authors.lastname: Yang
authors.forename: Wonshik
authors.initials: W
authors.lastname: Choi
authors.forename: Tai Hyun
authors.initials: TH
authors.lastname: Yoon
authors.forename: Kyoung Jin
authors.initials: KJ
authors.lastname: Lee
authors.forename: Jae-Seung
authors.initials: JS
authors.lastname: Lee
authors.forename: Sang Hun
authors.initials: SH
authors.lastname: Han
authors.forename: Min-Goo
authors.initials: MG
authors.lastname: Lee
authors.forename: Hong Soon
authors.initials: HS
authors.lastname: Yim
authors.forename: Kyung Min
authors.initials: KM
authors.lastname: Choi
authors.forename: Min Woo
authors.initials: MW
authors.lastname: Park
authors.forename: Kwang-Yoon
authors.initials: KY
authors.lastname: Jung
authors.forename: Seung-Kuk
authors.initials: SK
authors.lastname: Baek
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D003199:Computer Systems; D003287:Contrast Media / Q000737:chemistry / Q000627:therapeutic use; D006046:Gold / Q000627:therapeutic use*; D006258:Head and Neck Neoplasms / Q000473:pathology / Q000628:therapy*; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D008264:Macrophages / Q000737:chemistry / Q000637:transplantation*; D053768:Metal Nanoparticles / Q000627:therapeutic use*; D051379:Mice; D008858:Microscopy, Phase-Contrast / Q000379:methods; D010789:Phototherapy / Q000379:methods*; D016896:Treatment Outcome
chemical_list: D003287:Contrast Media; D006046:Gold