title: Complete fiber structures of complex trimeric autotransporter adhesins conserved in enterobacteria.
abstract: Trimeric autotransporter adhesins (TAAs) are modular, highly repetitive surface proteins that mediate adhesion to host cells in a broad range of Gram-negative pathogens. Although their sizes may differ by more than one order of magnitude, they all follow the same basic head-stalk-anchor architecture, where the head mediates adhesion and autoagglutination, the stalk projects the head from the bacterial surface, and the anchor provides the export function and attaches the adhesin to the bacterial outer membrane after export is complete. In complex adhesins, head and stalk domains may alternate several times before the anchor is reached. Despite extensive sequence divergence, the structures of TAA domains are highly constrained, due to the tight interleaving of their constituent polypeptide chains. We have therefore taken a "domain dictionary" approach to characterize representatives for each domain type by X-ray crystallography and use these structures to reconstruct complete TAA fibers. With SadA from Salmonella enterica, EhaG from enteropathogenic Escherichia coli (EHEC), and UpaG from uropathogenic E. coli (UPEC), we present three representative structures of a complex adhesin that occur in a conserved genomic context in Enterobacteria and is essential in the infection process of uropathogenic E. coli. Our work proves the applicability of the dictionary approach to understanding the structure of a class of proteins that are otherwise poorly tractable by high-resolution methods and provides a basis for the rapid and detailed annotation of newly identified TAAs.
authors.affiliation: Department of Protein Evolution,  Max Planck Institute for Developmental Biology, 72076 Tübingen,  Germany.
authors.forename: Marcus D
authors.initials: MD
authors.lastname: Hartmann
authors.forename: Iwan
authors.initials: I
authors.lastname: Grin
authors.forename: Stanislaw
authors.initials: S
authors.lastname: Dunin-Horkawicz
authors.forename: Silvia
authors.initials: S
authors.lastname: Deiss
authors.forename: Dirk
authors.initials: D
authors.lastname: Linke
authors.forename: Andrei N
authors.initials: AN
authors.lastname: Lupas
authors.forename: Birte
authors.initials: B
authors.lastname: Hernandez Alvarez
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry*; D000595:Amino Acid Sequence; D001692:Biological Transport; D018360:Crystallography, X-Ray / Q000379:methods; D054308:Enteropathogenic Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D019779:Salmonella enterica / Q000378:metabolism*
chemical_list: D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; D010455:Peptides