title: [Antigenic comparative analysis of Newcastle disease viruses with evolutional mutations in HN and F genes under antibody immune pressures].
abstract: In chicken fibroblast cell (CEF) cultures with antiserum against Newcastle disease virus (NDV) strain TZ060107, the virus was passed serially for 50 passages in 3 independent lineages. HN and F genes were amplified and sequenced every 10 passages. The derived virus A1-50 with most mutations among 3 lineages was further passed for another 50 passages in CEF with or without antiserum against A1-50, each in 3 independent lineages. Sequence comparisons for HN and F genes of 60, 70, 80, 90 and 100 passages indicated that the ratio of nonsynonymous mutations (NS) vs synonymous mutations (S) for HN genes in the lineages passed with antiserum against A1-50 was 5.25, which was obviously higher than 2. 375 of NS/ S in the lineages without the antiserum. The stable NS mutations occurred in the first 50 passages with the antiserum against the original TZ060107 were still maintained and one more new stable NS mutation appeared. For the F gene, 3 new stable NS mutations occurred during the second 50 passages in lineages with antiserum against A1-50 when the original NS mutations obtained in the first 50 passages with antiserum against TZ060107 still existed. Cross hemagglutination inhibition (HI) between original virus and its derivative viruses indicated that the more continuous passages in cell culture with antiserum passed, the bigger difference of antigenicity between the virus and the original virus had.
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China. hyting800@163.com
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: He
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Gong
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.forename: Zhi-Zhong
authors.initials: ZZ
authors.lastname: Cui
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D001483:Base Sequence; D002645:Chickens; D019143:Evolution, Molecular; D015707:HN Protein / Q000235:genetics* / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D008969:Molecular Sequence Data; D009154:Mutation; D009521:Newcastle Disease / Q000276:immunology / Q000821:virology*; D009522:Newcastle disease virus / Q000235:genetics* / Q000276:immunology; D011201:Poultry Diseases; D014760:Viral Fusion Proteins / Q000235:genetics* / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D015707:HN Protein; D014760:Viral Fusion Proteins