title: Influence of carboxylic acid functionalities in ruthenium (II) polypyridyl complexes on DNA binding, cytotoxicity and antioxidant activity: synthesis, structure and in vitro anticancer activity.
abstract: Four new Ru(II) complexes [RuHCl(bpy)(PPh(3))(CO)] (1), [RuHCl(bpy)(AsPh(3))(CO)] (2) (bpy = 2,2'-bipyridine), [RuCl(HL)(PPh(3))(2)(CO)] (3) and [RuCl(HL)(AsPh(3))(2)(CO)] (4) (HL = 2,2'-bipyridine-4,4'-dicarboxylic acid) were synthesized and characterized. X-ray diffraction was used to characterize 3 in solid state. The interactions of these complexes with DNA were explored by different techniques which revealed that the complexes could bind to CT-DNA through non-intercalation. The in vitro cytotoxic and antioxidant activities of the complexes validated against a panel of cancer cell lines and free radicals showed that 3 and 4 possess quite high anticancer and antioxidant activities over 1, 2 and standard drugs. An apparent dependence of biological activities on incorporation of COOH in bipyridine moiety was noticed: Inclusion of COOH caused significant differences in DNA binding, cytotoxicity and antioxidant activity.
authors.affiliation: Department of Chemistry, Bharathiar University, Coimbatore 641046, Tamilnadu, India.
authors.forename: Thangavel
authors.initials: T
authors.lastname: Sathiya Kamatchi
authors.forename: Nataraj
authors.initials: N
authors.lastname: Chitrapriya
authors.forename: Seog K
authors.initials: SK
authors.lastname: Kim
authors.forename: Frank R
authors.initials: FR
authors.lastname: Fronczek
authors.forename: Karuppannan
authors.initials: K
authors.lastname: Natarajan
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry*; D002264:Carboxylic Acids / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry*; D006801:Humans; D020128:Inhibitory Concentration 50; D008958:Models, Molecular; D015394:Molecular Structure; D011485:Protein Binding / Q000187:drug effects; D011725:Pyridines / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D002264:Carboxylic Acids; D056831:Coordination Complexes; D011725:Pyridines; D012428:Ruthenium; D004247:DNA