title: First report of Cowpea mild mottle Carlavirus on yardlong bean (Vigna unguiculata subsp. sesquipedalis) in Venezuela.
abstract: Yardlong bean (Vigna unguiculata subsp. sesquipedalis) plants with virus-like systemic mottling and leaf distortion were observed in both experimental and commercial fields in Aragua State, Venezuela. Symptomatic leaves were shown to contain  carlavirus-like particles. RT-PCR analysis with carlavirus-specific primers was positive in all tested samples. Nucleotide sequences of the obtained amplicons showed 84%-74% similarity to corresponding sequences of Cowpea mild mottle virus (CPMMV) isolates deposited in the GenBank database. This is the first report of CPMMV in Venezuela and is thought to be the first report of CPMMV infecting yardlong bean.
authors.affiliation: Central University of Venezuela, Agronomy Faculty, Institute of Agricultural Botany, Vegetal Virology and Phytopathogenic Bacteria Laboratory, ZIP 4579, Maracay 2101-A, Venezuela. britom@agr.ucv.ve
authors.forename: Miriam
authors.initials: M
authors.lastname: Brito
authors.forename: Thaly
authors.initials: T
authors.lastname: Fernández-Rodríguez
authors.forename: Mario José
authors.initials: MJ
authors.lastname: Garrido
authors.forename: Alexander
authors.initials: A
authors.lastname: Mejías
authors.forename: Mirtha
authors.initials: M
authors.lastname: Romano
authors.forename: Edgloris
authors.initials: E
authors.lastname: Marys
mesh_terms: D017788:Carlavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016000:Cluster Analysis; D007887:Fabaceae / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014687:Venezuela
chemical_list: D012367:RNA, Viral