title: Batch and fed-batch growth of Pichia pastoris under increased air pressure.
abstract: Pichia pastoris CBS 2612 behavior under air pressures of 1, 3 and 5 bar in culture media of glycerol (pure and crude) and methanol was studied. Generally, the increase in oxygen transfer rate due to the increase of total pressure improved cellular growth for all carbon sources and for batch and fed-batch processes with different feeding rate strategies. In batch cultures, 1.4-, 1.2-, and 1.5-fold improvement in biomass production was obtained with the increase of air pressure up to 5 bar, using methanol, pure glycerol, and crude glycerol, respectively. The increase of air pressure to 5 bar using exponential feeding rate led to 1.4-fold improvement in biomass yield per glycerol mass consumed, for crude and pure glycerol. The current low cost of crude glycerol from the biodiesel production together with the present results shows the possibility of improving cell mass production of P. pastoris using increased air pressure.
authors.affiliation: Centre of Biological Engineering, Institute for Biotechnology and Bioengineering IBB, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: Marlene
authors.initials: M
authors.lastname: Lopes
authors.forename: Isabel
authors.initials: I
authors.lastname: Belo
authors.forename: Manuel
authors.initials: M
authors.lastname: Mota
mesh_terms: D000399:Air Pressure; D018533:Biomass; D003451:Cryoprotective Agents / Q000378:metabolism / Q000494:pharmacology; D005990:Glycerol / Q000378:metabolism / Q000494:pharmacology; D010843:Pichia / Q000254:growth & development*
chemical_list: D003451:Cryoprotective Agents; D005990:Glycerol