title: Dynamic changes in heat transducing channel TRPV1 expression regulate mechanically insensitive, heat sensitive C-fiber recruitment after axotomy and regeneration.
abstract: Peripheral injury leads to a significant increase in the prevalence of mechanically insensitive, heat-sensitive C-fibers (CH) that contain the heat transducing TRPV1 (transient receptor potential vanilloid type I) channel in mice. We have recently shown that this recruitment of CH fibers is associated with increased expression of the receptor for GDNF (glial cell line-derived neurotrophic factor) family neurotrophic factor artemin (GFRα3), and that in vivo inhibition of GFRα3 prevented the increase in TRPV1 expression normally observed following axotomy. Here we have directly tested the hypothesis that the recruitment of functional CH fibers following nerve regeneration requires enhanced TRPV1 levels. We used in vivo siRNA-mediated knockdown to inhibit the injury-induced expression of TRPV1 coupled with ex vivo recording to examine response characteristics and neurochemical phenotypes of different functionally defined cutaneous sensory neurons after regeneration. We confirmed that inhibition of TRPV1 did not affect the axotomy-induced decrease in polymodal C-fiber (CPM) heat threshold, but transiently prevented the recruitment of CH neurons. Moreover, a recovery of TRPV1 protein was observed following resolution of siRNA-mediated inhibition that was correlated with a concomitant rebound in CH neuron recruitment. Thus dynamic changes in TRPV1 expression, not absolute levels, may underlie the functional alterations observed in CH neurons and may contribute to the development of heat hyperalgesia after nerve injury.
authors.affiliation: Department of Neurobiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Jankowski
authors.forename: Deepak J
authors.initials: DJ
authors.lastname: Soneji
authors.forename: Katrina M
authors.initials: KM
authors.lastname: Ekmann
authors.forename: Collene E
authors.initials: CE
authors.lastname: Anderson
authors.forename: H Richard
authors.initials: HR
authors.lastname: Koerber
mesh_terms: D000818:Animals; D019771:Axotomy / Q000379:methods; D055785:Gene Knockdown Techniques / Q000379:methods; D006358:Hot Temperature; D008297:Male; D051379:Mice; D036421:Nerve Fibers, Unmyelinated / Q000378:metabolism / Q000502:physiology*; D009416:Nerve Regeneration / Q000502:physiology*; D034741:RNA, Small Interfering / Q000494:pharmacology; D011999:Recruitment, Neurophysiological / Q000502:physiology*; D011984:Sensory Receptor Cells / Q000502:physiology*; D012684:Sensory Thresholds / Q000502:physiology; D050916:TRPV Cation Channels / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000502:physiology*
chemical_list: D034741:RNA, Small Interfering; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse