title: Specific properties of enteropathogenic Escherichia coli isolates from diarrheal patients and comparison to strains from foods and fecal specimens from cattle, swine, and healthy carriers in Osaka City, Japan.
abstract: For exhaustive detection of diarrheagenic Escherichia coli, we previously developed a colony-hybridization method using hydrophobic grid-membrane filters in combination with multiplex real-time PCR. To assess the role of domestic animals as the source of atypical enteropathogenic E. coli (aEPEC), a total of 679 samples (333 from foods, fecal samples from 227 domestic animals, and 119 from healthy people) were examined. Combining 48 strains previously isolated from patients and carriers, 159 aEPEC strains were classified by phylogroup, virulence profile, and intimin typing. Phylogroup B1 was significantly more prevalent among aEPEC from patients (50%) and bovine samples (79%) than from healthy carriers (16%) and swine strains (23%), respectively. Intimin type β1 was predominant in phylogroup B1; B1-β1 strains comprised 26% of bovine strains and 25% of patient strains. The virulence profile groups Ia and Ib were also observed more frequently among bovine strains than among porcine strains. Similarly, virulence group Ia was detected more frequently among patient strains than strains of healthy carriers. A total of 85 strains belonged to virulence group I, and 63 of these strains (74%) belonged to phylogroup B1. The present study suggests that the etiologically important aEPEC in diarrheal patients could be distinguished from aEPEC strains indigenous to humans based on type, such as B1, Ia, and β1/γ1, which are shared with bovine strains, while the aEPEC strains in healthy humans are different, and some of these were also present in porcine samples.
authors.affiliation: Osaka City University Graduate School of Human Life Science, Osaka, Japan.
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.forename: Mitsuko
authors.initials: M
authors.lastname: Wakushima
authors.forename: Tetsu
authors.initials: T
authors.lastname: Aota
authors.forename: Yuka
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Toshimasa
authors.initials: T
authors.lastname: Kita
authors.forename: Tomofumi
authors.initials: T
authors.lastname: Maehara
authors.forename: Jun
authors.initials: J
authors.lastname: Ogasawara
authors.forename: Changsun
authors.initials: C
authors.lastname: Choi
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Kamata
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Hara-Kudo
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Nishikawa
mesh_terms: D000818:Animals; D002353:Carrier State / Q000382:microbiology; D002417:Cattle / Q000382:microbiology*; D016000:Cluster Analysis; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology; D054308:Enteropathogenic Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005516:Food Microbiology; D005838:Genotype; D006801:Humans; D007564:Japan / Q000453:epidemiology; D017720:Molecular Epidemiology; D058889:Molecular Typing; D010802:Phylogeny; D013552:Swine / Q000382:microbiology*; D037521:Virulence Factors / Q000235:genetics
chemical_list: D037521:Virulence Factors