title: Altered expression levels of IDH2 are involved in the development of colon cancer.
abstract: IDH2 encodes a mitochondrial metabolic enzyme that converts isocitrate to α-ketoglutarate (α-KG) by reducing nicotinamide adenine dinucleotide phosphate (NADP(+)) to NADPH and participates in the citric acid cycle for energy production. Notably, this gene has been shown to be critical for cell proliferation. The abnormal expression of IDH2 has been reported in several types of cancer, and mutations in IDH2 have been identified in gliomas and acute myelogenous leukemia. The overexpression of IDH2 has been reported in endometrial, prostate and testicular cancer as well as in Kashin-Beck disease. In this study, we observed that IDH2 expression was significantly downregulated in early phase but was upregulated in advanced phase colon carcinoma compared to peritumoral tissues. In addition, we demonstrated that the growth of a colon carcinoma cell line was inhibited by IDH2-siRNA and increased following transfection with an IDH2-overexpressing plasmid. These results indicate that IDH2 may play a unique role in the development of colon carcinoma.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062;
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lv
authors.forename: Shenyang
authors.initials: S
authors.lastname: Xing
authors.forename: Zhenxiao
authors.initials: Z
authors.lastname: Li
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
authors.forename: Pengtao
authors.initials: P
authors.lastname: Gong
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Xu
authors.forename: LE
authors.initials: L
authors.lastname: Chang
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Jin
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Guocai
authors.initials: G
authors.lastname: Zhang
authors.forename: Ju
authors.initials: J
authors.lastname: Yang
authors.forename: Xichen
authors.initials: X
authors.lastname: Zhang