title: [Extraction and analysis of the essential oil in Pogostemon cablin by enzymatic hydrolysis and inhibitory activity against Hela cell proliferation].
abstract: OBJECTIVE
  To optimize the extraction method of essential oil in Pogostemon cablin and analyze its inhibitory activity against Hela cell proliferation.
  
  
  METHODS
  The Pogostemon cablin was treated by hemicellulase before steam distillation. The enzyme dosage, treatment time, treatment temperature, pH were optimized through orthogonal experimental design. The components of essential oil were identified by gas chromatography-mass spectrometry (GC-MS). Inhibitory activity of patchouli oil against Hela cell proliferation was determined by MTP method.
  
  
  RESULTS
  The optimum extraction process was as follows: pH 4.5, temperature 45 degrees C, the ratio of hemicellulase to Pogostemon cablin was 1% and enzymatic hydrolysis for 1.0 hour. Extraction ratio of the patchouli oil in steam distillation and hemicellulase extraction method was 2.2220 mg/g, 3.1360 mg/g respectively. Patchouli oil could inhibit Hela cell proliferation. IC50 of the patchouli oil in steam distillation and hemicellulase extraction method was 12.2 +/- 0.46 microg/mL and 0.36 +/- 0.03 microg/mL respectively.
  
  
  CONCLUSIONS
  In comparison with steam distillation method, extraction ratios of essential oil and the inhibitory activity against Hela cell proliferation can be increased by the hemicellulase extraction method.
authors.affiliation: Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, China. bingmeigui1986@sina.com
authors.forename: Jing
authors.initials: J
authors.lastname: Yu
authors.forename: Yue
authors.initials: Y
authors.lastname: Qi
authors.forename: Gang
authors.initials: G
authors.lastname: Luo
authors.forename: Hong-quan
authors.initials: HQ
authors.lastname: Duan
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D057168:Distillation / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry; D006026:Glycoside Hydrolases / Q000008:administration & dosage / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D006868:Hydrolysis; D020128:Inhibitory Concentration 50; D019686:Lamiaceae / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013227:Steam; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D009822:Oils, Volatile; D013227:Steam; D006026:Glycoside Hydrolases; C023305:hemicellulase