title: Enhanced mid-infrared chemical imaging (IRCI) detection of DNA microarrays.
abstract: We report on the optimization of a recently proposed mid-infrared chemical imaging (IRCI) detection method for the analysis of DNA microarrays. The improved protocol allowed for a ten-fold reduction in the time needed to generate a mosaic image of an entire microarray and the production  of IR images with high contrast that would facilitate data analysis and interpretation. Advantages of using this protocol were evaluated by applying it to the analysis of four virulence genes in the genomes of 19 strains of the food bacterial pathogen Yersinia enterocolitica.
authors.affiliation: Division of Microbiology, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, MD 20740-3835, USA.
authors.forename: Brianna
authors.initials: B
authors.lastname: Schoen
authors.forename: Magdi M
authors.initials: MM
authors.lastname: Mossoba
authors.forename: Vladimir
authors.initials: V
authors.lastname: Chizhikov
authors.forename: Anis
authors.initials: A
authors.lastname: Rashid
authors.forename: Karen
authors.initials: K
authors.lastname: Martinez-Diaz
authors.forename: Sufian F
authors.initials: SF
authors.lastname: Al-Khaldi
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry; D005798:Genes, Bacterial; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D013055:Spectrophotometry, Infrared / Q000379:methods*; D014774:Virulence; D015008:Yersinia enterocolitica / Q000737:chemistry / Q000145:classification / Q000235:genetics
chemical_list: D004269:DNA, Bacterial