title: Catalasic activity in fish liver: improvement of the UV to visible analytic method.
abstract: Antioxidative defenses and more especially catalasic activity (CAT) are studied in a large range of scientific research thematics. In environmental sciences, the problematic of oxidative stress is of great interest as pollutants can induce perturbations of redox homeostasis. Consequently, changes in antioxidative defenses levels in fish tissues and particularly in liver are used as potential biomarkers of pollution. In most studies, the CAT was assayed by following during 5 min the consumption of H2O2 in cytosolic buffered extracts at 240 nm (UV-method). This study proposed a development of this method in the visible, using permanganate and a 525-nm detection, which was more accurate, sensitive, and rapid. Moreover, the hepatic CAT of six different fish species [a cyclidae (Nimbochromis linni), 3 cyprinidae (Brachydanio rerio, Rutilus rutilus, Cyprinus carpio), an anguillidae (Anguilla anguilla), and a percidae (Perca fluviatilus)] was evaluated with the two protocols (UV- and KMnO4-method). The results but also the thermal optimum of the reaction and the interest of CAT as biomarker in ecotoxicology were discussed.
authors.affiliation: Laboratoire d'Ecologie-Ecotoxicologie, Faculté des Sciences, EA4689 Interaction Animal-Environnement, Université de Reims Champagne-Ardenne, Moulin de Housse, BP 1039-51687, Reims Cedex 2, France. severine.paris@univ-reims.fr
authors.forename: Séverine
authors.initials: S
authors.lastname: Paris-Palacios
authors.forename: Laurence
authors.initials: L
authors.lastname: Delahaut
authors.forename: Alexis
authors.initials: A
authors.lastname: Carreras
authors.forename: Marielle
authors.initials: M
authors.lastname: Thomas
authors.forename: Sylvie
authors.initials: S
authors.lastname: Biagianti-Risbourg
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism*; D004784:Environmental Monitoring; D005399:Fishes / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D011196:Potassium Permanganate; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
chemical_list: D011196:Potassium Permanganate; D002374:Catalase