title: Multi-parametric imaging of murine brain using spectral and time domain optical coherence tomography.
abstract: Examination of brain functions in small animal models may help improve the diagnosis and treatment of neurological conditions. Transcranial imaging of small rodents' brains poses a major challenge for optical microscopy. Another challenge is to reduce the measurement time. We describe methods and algorithms for three-dimensional assessment of blood flow in the brains of small animals, through the intact skull, using spectral and time domain optical coherence tomography. By introducing a resonant scanner to the optical setup of the optical coherence tomography (OCT) system, we have developed and applied a high-speed spectral OCT technique that allows us to vary the imaging range of flow and to shorten measurement time. Multi-parameter signal analysis enables us to obtain both qualitative and quantitative information about flow velocity from the same set of data.
authors.affiliation: Institute of Physics, Nicolaus Copernicus University, Grudziadzka 5, PL-87-100 Torun, Poland.
authors.forename: Danuta
authors.initials: D
authors.lastname: Bukowska
authors.forename: Daniel
authors.initials: D
authors.lastname: Ruminski
authors.forename: Daniel
authors.initials: D
authors.lastname: Szlag
authors.forename: Ireneusz
authors.initials: I
authors.lastname: Grulkowski
authors.forename: Jakub
authors.initials: J
authors.lastname: Wlodarczyk
authors.forename: Maciej
authors.initials: M
authors.lastname: Szkulmowski
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Wilczynski
authors.forename: Iwona
authors.initials: I
authors.lastname: Gorczynska
authors.forename: Maciej
authors.initials: M
authors.lastname: Wojtkowski
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply*; D002560:Cerebrovascular Circulation / Q000502:physiology; D004301:Doppler Effect; D005260:Female; D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D041623:Tomography, Optical Coherence / Q000379:methods*