title: An integrated solution for rapid biosensing with robust linker free covalent binding surfaces.
abstract: A novel integrated biosensor methodology is proposed and demonstrated. The methodology utilizes a nitrogen-containing plasma polymer to achieve linker-free binding of a layer of biorecognition molecules. The sensor surface has been shown to maintain its performance after freeze-drying providing a long shelf life under ambient conditions. The sensor is configured for single wavelength ellipsometric detection providing a low cost, versatile, and rapid sensing and diagnosis platform suitable for a wide range of applications and end-users. The merits of the methodology are demonstrated using three antigen-antibody pairs.
authors.affiliation: School of Physics, University of Sydney, NSW 2006, Australia. yyin@physics.usyd.edu.au
authors.forename: Yongbai
authors.initials: Y
authors.lastname: Yin
authors.forename: Marcela M M
authors.initials: MM
authors.lastname: Bilek
authors.forename: Keith
authors.initials: K
authors.lastname: Fisher
authors.forename: Chuan
authors.initials: C
authors.lastname: Guo
authors.forename: David R
authors.initials: DR
authors.lastname: McKenzie
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D006801:Humans; D009584:Nitrogen / Q000737:chemistry*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D013499:Surface Properties
chemical_list: D011506:Proteins; D009584:Nitrogen