title: Prokaryotic expression of the truncated duck enteritis virus UL27 gene and characteristics of UL27 gene and its truncated product.
abstract: Duck enteritis virus (DEV) is an acute and contagious herpesvirus infection in waterfowls, which leads to heavy economic losses in the commercial duck industry due to its high mortality rate and decreasing duck egg production. In order to understand this virus better, truncated UL27 gene (tUL27) was used to construct pET-28a-tUL27 recombinant plasmid. The tUL27 gene product, truncated gB protein (tgB), was used to prepare rabbit anti-tgB protein polyclonal antibody. The polyclonal antibody was used for characteristics of DEV glycoprotein B (gB) intracellular localization. Protein gB was firstly detected in nuclear membrane of the infected DEF, and later during the infection it appeared as the punctiform fluorescence in cytoplasm. We also proved that DEV gB is a late protein. Localization of DEV gB in nuclear membrane, suggested a possible role in virus assembly, while appearance in cytoplasm suggests its role in viral egress. All these data provide a basal and useful knowledge about the functions of DEV UL27 gene and its product gB.
authors.affiliation: Sichuan Agricultural University, Wenjiang, Sichuan, People's Republic of China.
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.forename: D
authors.initials: D
authors.lastname: Lin
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: D
authors.initials: D
authors.lastname: Zhu
authors.forename: R
authors.initials: R
authors.lastname: Jia
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.forename: A
authors.initials: A
authors.lastname: Cheng
mesh_terms: D000818:Animals; D004372:Ducks; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D025922:Mardivirus / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D011201:Poultry Diseases / Q000821:virology*; D021381:Protein Transport; D011817:Rabbits; D017384:Sequence Deletion; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D014764:Viral Proteins