title: Blueberry polyphenol-enriched soybean flour reduces hyperglycemia, body weight gain and serum cholesterol in mice.
abstract: Defatted soybean flour (DSF) can sorb and concentrate blueberry anthocyanins and other polyphenols, but not sugars. In this study blueberry polyphenol-enriched DSF (BB-DSF) or DSF were incorporated into very high fat diet (VHFD) formulations and provided ad libitum to obese and hyperglycemic C57BL/6 mice for 13 weeks to investigate anti-diabetic effects. Compared to the VHFD containing DSF, the diet supplemented with BB-DSF reduced weight gain by 5.6%, improved glucose tolerance, and lowered fasting blood glucose levels in mice within 7 weeks of intervention. Serum cholesterol of mice consuming the BB-DSF-supplemented diet was 13.2% lower than mice on the diet containing DSF. Compounds were eluted from DSF and BB-DSF for in vitro assays of glucose production and uptake. Compared to untreated control, doses of BB-DSF eluate containing 0.05-10μg/μL of blueberry anthocyanins significantly reduced glucose production by 24-74% in H4IIE rat hepatocytes, but did not increase glucose uptake in L6 myotubes. The results indicate that delivery of blueberry polyphenols stabilized in a high-protein food matrix may be useful for the dietary management of pre-diabetes and/or diabetes.
authors.affiliation: Rutgers, The State University of New Jersey, School of Environmental and Biological Sciences, Foran Hall, 59 Dudley Road, New Brunswick, NJ 08901, USA. roopchand@aesop.rutgers.edu
authors.forename: Diana E
authors.initials: DE
authors.lastname: Roopchand
authors.forename: Peter
authors.initials: P
authors.lastname: Kuhn
authors.forename: Leonel E
authors.initials: LE
authors.lastname: Rojo
authors.forename: Mary Ann
authors.initials: MA
authors.lastname: Lila
authors.forename: Ilya
authors.initials: I
authors.lastname: Raskin
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000494:pharmacology / Q000627:therapeutic use; D029798:Blueberry Plants; D002460:Cell Line; D045744:Cell Line, Tumor; D002784:Cholesterol / Q000097:blood; D005433:Flour; D005947:Glucose / Q000378:metabolism; D006943:Hyperglycemia / Q000188:drug therapy* / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D032446:Myoblasts / Q000378:metabolism; D059808:Polyphenols / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D013025:Glycine max; D015430:Weight Gain / Q000187:drug effects
chemical_list: D000872:Anthocyanins; D007004:Hypoglycemic Agents; D059808:Polyphenols; D002784:Cholesterol; D005947:Glucose