title: Identification of a phosphorylation site in cyclobutane pyrimidine dimer photolyase of rice.
abstract: Cyclobutane pyrimidine dimer (CPD) photolyase monomerises ultraviolet (UV) radiation-induced CPDs present in DNA, using energy from UVA and visible light. In plants, CPD photolyase activity is a crucial factor for determining UVB sensitivity. We previously demonstrated that native rice CPD photolyase is phosphorylated. To determine the phosphorylation site(s), the phosphorylation status of CPD photolyase was analyzed in rice varieties that have amino acid alterations at the potential phosphorylation sites. In wild-rice species, CPD photolyase was phosphorylated. In Poaceae species, CPD photolyase was phosphorylated in wheat but not in maize. Mutant CPD photolyase proteins, in which these putative phosphorylated residues were replaced with alanine residues, were synthesized using an insect cell-free translation system. A slow-migrating band disappeared when the serine residue at position 7 was mutated. A phospho-specific antibody was generated to determine whether this residue is phosphorylated in CPD photolyase. Only the slow-migrating band of native rice CPD photolyase was detected using this antibody, indicating that the serine residue at position 7 is a phosphorylation site in native rice CPD photolyase.
authors.affiliation: Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, Japan. tera@ige.tohoku.ac.jp
authors.forename: Mika
authors.initials: M
authors.lastname: Teranishi
authors.forename: Kentaro
authors.initials: K
authors.lastname: Nakamura
authors.forename: Haruya
authors.initials: H
authors.lastname: Furukawa
authors.forename: Jun
authors.initials: J
authors.lastname: Hidema
mesh_terms: D000595:Amino Acid Sequence; D020134:Catalytic Domain; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology*; D010766:Phosphorylation; D011740:Pyrimidine Dimers / Q000378:metabolism
chemical_list: D011740:Pyrimidine Dimers; D004255:Deoxyribodipyrimidine Photo-Lyase