title: SOCS1 abrogates IFN's antiviral effect on hepatitis C virus replication.
abstract: Suppressor of cytokine signaling 1 (SOCS1) and suppressor of cytokine signaling 3 (SOCS3) have been thought to block type I interferon (IFN) signaling. We have previously reported that SOCS3 suppresses HCV replication in an mTOR-dependent manner. However, the relationship between SOCS1 and HCV replication remains unclear. Here, we found that overexpression of SOCS1 alone did not have an effect on HCV RNA replication. However, suppression of HCV replication by IFN-α was rescued by SOCS1 overexpression. The upregulation of HCV replication by SOCS1 overexpression in the presence of IFN is likely a result of the impairment of IFN signaling by SOCS1 and subsequent induction of ISGs. Knockdown of SOCS1 alone with specific shRNA enhanced the antiviral effect of IFN compared with negative control. Thus, SOCS1 acts as a suppressor of type I IFN function against HCV.
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
authors.forename: Run-Xuan
authors.initials: RX
authors.lastname: Shao
authors.forename: Leiliang
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Hong
authors.forename: Kaku
authors.initials: K
authors.lastname: Goto
authors.forename: Du
authors.initials: D
authors.lastname: Cheng
authors.forename: Wen-Chi
authors.initials: WC
authors.lastname: Chen
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Jilg
authors.forename: Kattareeya
authors.initials: K
authors.lastname: Kumthip
authors.forename: Dahlene N
authors.initials: DN
authors.lastname: Fusco
authors.forename: Lee F
authors.initials: LF
authors.lastname: Peng
authors.forename: Raymond T
authors.initials: RT
authors.lastname: Chung
mesh_terms: D002460:Cell Line; D016174:Hepacivirus / Q000276:immunology / Q000502:physiology*; D022781:Hepatocytes / Q000821:virology; D054884:Host-Pathogen Interactions; D006801:Humans; D016898:Interferon-alpha / Q000037:antagonists & inhibitors* / Q000276:immunology*; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000276:immunology*; D014779:Virus Replication
chemical_list: D016898:Interferon-alpha; C108944:SOCS1 protein, human; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins