title: [A new monoterpenoid glucoside from the twigs of Chamaecyparis obtusa var. breviramea f. crippsii].
abstract: To investigate the chemical constituents of Chamaecyparis obtusa var. breviramea f. crippsii, various column chromatography and spectroscopic methods were used for the isolation and elucidation of compounds. One new monoterpenoid glucoside, (4S)-4-isopropylcyclohex-l-enecarboxylic acid 4-O-beta-D-glucopyranoside (1), together with five known compounds, (4R)-p-menth-l-ene-7, 8-diol 7-O-beta-D-glucopyranoside (2), skimmin (3), 7-[[6-O-(6-deoxy-alpha-L-mannopyranosyl)-beta-D-glucopyranosyl]oxy]-2H-1-benzopyran-2-one (4), stigmast-4-en-3-one (5) and 1, 4-benzenedicarboxylic acid 1-butyl-4-(2-methylpropyl) ester (6) were isolated and identified from the twigs of this plant. All compounds were isolated from this plant for the first time. The methanol extract of this plant showed cytotoxicity on cancer cell lines A549, BGC-823, Du145 and MDA-MB-231 with IC50 values of 0.94, 1.07, 0.95 and 0.96 microg x mL(-1), respectively. Yet, compounds 1, 2 and 3 showed no cytotoxicity on cancer cell lines HeLa, BGC-823 and A549.
authors.affiliation: Key Laboratory of Ministry of Education for Traditional Chinese Medicine Resource and Compound Prescription, Hubei University of Chinese Medicine, Wuhan 430065, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Zhang
authors.forename: Ke-Li
authors.initials: KL
authors.lastname: Chen
authors.forename: Ning-Hua
authors.initials: NH
authors.lastname: Tan
authors.forename: Yi-Mei
authors.initials: YM
authors.lastname: Liu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D029780:Chamaecyparis / Q000737:chemistry*; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D015394:Molecular Structure; D039821:Monoterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D013265:Stigmasterol / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: C583809:(4S)-4-isopropylcyclohex-l-enecarboxylic acid 4-O-beta-D-glucopyranoside; D000972:Antineoplastic Agents, Phytogenic; D003374:Coumarins; D005960:Glucosides; D039821:Monoterpenes; C488470:stigmast-4-en-3-one; D013265:Stigmasterol; C581268:skimmin