title: The importance of the interaction of CheD with CheC and the chemoreceptors compared to its enzymatic activity during chemotaxis in Bacillus subtilis.
abstract: Bacillus subtilis use three systems for adaptation during chemotaxis. One of these systems involves two interacting proteins, CheC and CheD. CheD binds to the receptors and increases their ability to activate the CheA kinase. CheD also binds CheC, and the strength of this interaction is increased by phosphorylated CheY. CheC is believed to control the binding of CheD to the receptors in response to the levels of phosphorylated CheY. In addition to their role in adaptation, CheC and CheD also have separate enzymatic functions. CheC is a CheY phosphatase and CheD is a receptor deamidase. Previously, we demonstrated that CheC's phosphatase activity plays a minor role in chemotaxis whereas its ability to bind CheD plays a major one. In the present study, we demonstrate that CheD's deamidase activity also plays a minor role in chemotaxis whereas its ability to bind CheC plays a major one. In addition, we quantified the interaction between CheC and CheD using surface plasmon resonance. These results suggest that the most important features of CheC and CheD are not their enzymatic activities but rather their roles in adaptation.
authors.affiliation: Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America.
authors.forename: Wei
authors.initials: W
authors.lastname: Yuan
authors.forename: George D
authors.initials: GD
authors.lastname: Glekas
authors.forename: George M
authors.initials: GM
authors.lastname: Allen
authors.forename: Hanna E
authors.initials: HE
authors.lastname: Walukiewicz
authors.forename: Christopher V
authors.initials: CV
authors.lastname: Rao
authors.forename: George W
authors.initials: GW
authors.lastname: Ordal
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000166:cytology* / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002268:Carboxypeptidases / Q000378:metabolism; D002628:Chemoreceptor Cells / Q000166:cytology* / Q000201:enzymology*; D002633:Chemotaxis; D018360:Crystallography, X-Ray; D057075:Enzyme Assays; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D011485:Protein Binding; D015203:Reproducibility of Results; D016415:Sequence Alignment; D020349:Surface Plasmon Resonance; D020124:Thermotoga maritima / Q000201:enzymology
chemical_list: D001426:Bacterial Proteins; C446291:CheC protein, Bacillus subtilis; C446292:CheD protein, Bacillus subtilis; D002268:Carboxypeptidases