title: Analysis of the extreme diversity of salivary alpha-amylase isoforms generated by physiological proteolysis using liquid chromatography-tandem mass spectrometry.
abstract: Saliva is a crucial biofluid for oral health and is also of increasing importance as a non-invasive source of disease biomarkers. Salivary alpha-amylase is an abundant protein in saliva, and changes in amylase expression have been previously associated with a variety of diseases and conditions. Salivary alpha-amylase is subject to a high diversity of post-translational modifications, including physiological proteolysis in the oral cavity. Here we developed methodology for rapid sample preparation and non-targeted LC-ESI-MS/MS analysis of saliva from healthy subjects and observed an extreme diversity of alpha-amylase proteolytic isoforms. Our results emphasize the importance of consideration of post-translational events such as proteolysis in proteomic studies, biomarker discovery and validation, particularly in saliva.
authors.affiliation: School of Chemistry & Molecular Biosciences, The University of Queensland, Brisbane, Queensland 4072, Australia.
authors.forename: Ulla-Maja
authors.initials: UM
authors.lastname: Bailey
authors.forename: Chamindie
authors.initials: C
authors.lastname: Punyadeera
authors.forename: Justin J
authors.initials: JJ
authors.lastname: Cooper-White
authors.forename: Benjamin L
authors.initials: BL
authors.lastname: Schulz
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D015415:Biomarkers / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D020033:Protein Isoforms; D015203:Reproducibility of Results; D012463:Saliva / Q000201:enzymology*; D055291:Salivary alpha-Amylases / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014357:Trypsin / Q000378:metabolism
chemical_list: D015415:Biomarkers; D010446:Peptide Fragments; D020033:Protein Isoforms; D055291:Salivary alpha-Amylases; D014357:Trypsin