title: Isolation and characterization of eleven polymorphic microsatellite loci for the valuable medicinal plant Dendrobium huoshanense and cross-species amplification.
abstract: Dendrobium huoshanense (Orchidaceae) is a perennial herb and a widely used medicinal plant in Traditional Chinese medicine (TCM) endemic to Huoshan County town in Anhui province in Southeast China. A microsatellite-enriched genomic DNA library of D. huoshanense was developed and screened to identify marker loci. Eleven polymorphic loci were isolated and analyzed by screening 25 individuals collected from a natural population. The number of alleles per locus ranged from 2 to 5. The observed  and expected heterozygosities ranged from 0.227 to 0.818 and from 0.317 to 0.757, respectively. Two loci showed significant deviations from Hardy-Weinberg equilibrium and four of the pairwise comparisons of loci revealed linkage disequilibrium (p < 0.05). These microsatellite loci were cross-amplified for five congeneric species and seven loci can be amplified in all species. These simple sequence repeats (SSR) markers are useful  in genetic studies of D. huoshanense and other related species and in conservation  decision-making.
authors.affiliation: Key Laboratory of Molecular Evolution and Biodiversity and Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, College of Life Science, Anhui Normal University, Wuhu 241000, China. gpz1996@yahoo.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Nai-Fu
authors.initials: NF
authors.lastname: Chen
authors.forename: Ji-Yang
authors.initials: JY
authors.lastname: Zheng
authors.forename: Wen-Cai
authors.initials: WC
authors.lastname: Wang
authors.forename: Yun-Yun
authors.initials: YY
authors.lastname: Pei
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Zhu
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D031667:Dendrobium / Q000235:genetics*; D056426:Genetic Loci; D018895:Microsatellite Repeats / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D010946:Plants, Medicinal / Q000235:genetics; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013045:Species Specificity