title: Comprehensive cytogenomic profile of the in vitro neuronal model SH-SY5Y.
abstract: The widely studied SH-SY5Y human neuroblastoma cell line provides a classic example of how a cancer cell line can be instrumental for discoveries of broad biological and clinical significance. An important feature of the SH-SY5Y cells is their ability to differentiate into a functionally mature neuronal phenotype. This property has conferred them the potential to be used as an in vitro model for studies of neurodegenerative and neurodevelopmental disorders. Here, we present a comprehensive assessment of the SH-SY5Y cytogenomic profile. Our results advocate for molecular cytogenetic data to inform the use of cancer cell lines in research.
authors.affiliation: Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, OX3 7BN, Oxford, UK.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Yusuf
authors.forename: Kay
authors.initials: K
authors.lastname: Leung
authors.forename: Keith J
authors.initials: KJ
authors.lastname: Morris
authors.forename: Emanuela V
authors.initials: EV
authors.lastname: Volpi
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D055028:Comparative Genomic Hybridization; D020732:Cytogenetic Analysis; D018628:Gene Dosage; D023281:Genomics / Q000379:methods; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008962:Models, Theoretical; D009447:Neuroblastoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism* / Q000473:pathology