title: The overexpression of nuclear envelope protein Lap2β induces endoplasmic reticulum reorganisation via membrane stacking.
abstract: Some nuclear envelope proteins are localised to both the nuclear envelope and the endoplasmic reticulum; therefore, it seems plausible that even small amounts of these proteins can influence the organisation of the endoplasmic reticulum. A simple method to study the possible effects of nuclear envelope proteins on endoplasmic reticulum organisation is to analyze nuclear envelope protein overexpression. Here, we demonstrate that Lap2β overexpression can induce the formation of cytoplasmic vesicular structures derived from endoplasmic reticulum membranes. Correlative light and electron microscopy demonstrated that these vesicular structures were composed of a series of closely apposed membranes that were frequently arranged in a circular fashion. Although stacked endoplasmic reticulum cisternae were highly ordered, Lap2β could readily diffuse into and out of these structures into the surrounding reticulum. It appears that low-affinity interactions between cytoplasmic domains of Lap2β can reorganise reticular endoplasmic reticulum into stacked cisternae. Although the effect of one protein may be insignificant at low concentrations, the cumulative effect of many non-specialised proteins may be significant.
authors.affiliation: Lomonosov Moscow State University ,  119991 Moscow ,  Russia.
authors.forename: Ekaterina G
authors.initials: EG
authors.lastname: Volkova
authors.forename: Sergey S
authors.initials: SS
authors.lastname: Abramchuk
authors.forename: Eugene V
authors.initials: EV
authors.lastname: Sheval