title: Very early effect of DMBA and MNU on microRNA expression.
abstract: BACKGROUND
  microRNA expression profile analysis provides evaluation of the early stages of carcinogenesis. This study focuses on early alteration of miRNA expression after treatment with different carcinogens.
  
  
  METHODS
  Mice were intraperitoneally injected with one dose of 7,12-dimethylbenz(α)anthracene (DMBA) and N-methyl-N-nitrosourea (MNU). The expression of miRNAs were analyzed 3 and 6 hours after the treatment, using quantitative real-time-polymerase chain reaction.
  
  
  RESULTS
  Underexpression of miR-34a and miR-155 were detected in the liver, spleen and kidneys at 3 and 6 hours after MNU treatment. In the lungs and kidneys, the expression of miR-21 was significantly elevated 6 hours after DMBA treatment, while in the liver, MNU induced higher expression levels of miR-21 at 3 and 6 hours compared to treatment with DMBA.
  
  
  CONCLUSIONS
  The different response of miRNAs to carcinogens emphasizes their possible role as potential epidemiological biomarkers in early phases of environmental tumorigenesis.
authors.affiliation: Institute of Public Health, Faculty of Medicine, University of Pécs, Pécs, 12 Szigeti str., 7624 Hungary. krisztina.juhasz01@gmail.com
authors.forename: Krisztina
authors.initials: K
authors.lastname: Juhász
authors.forename: Katalin
authors.initials: K
authors.lastname: Gombos
authors.forename: Mónika
authors.initials: M
authors.lastname: Szirmai
authors.forename: Katalin
authors.initials: K
authors.lastname: Gőcze
authors.forename: Veronika
authors.initials: V
authors.lastname: Wolher
authors.forename: Péter
authors.initials: P
authors.lastname: Révész
authors.forename: Ingrid
authors.initials: I
authors.lastname: Magda
authors.forename: Andor
authors.initials: A
authors.lastname: Sebestyén
authors.forename: Arpád
authors.initials: A
authors.lastname: Németh
authors.forename: István
authors.initials: I
authors.lastname: Ember
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity*; D000477:Alkylating Agents / Q000633:toxicity; D000818:Animals; D002273:Carcinogens / Q000633:toxicity; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D008770:Methylnitrosourea / Q000633:toxicity*; D051379:Mice; D008808:Mice, Inbred CBA; D035683:MicroRNAs / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
chemical_list: D000477:Alkylating Agents; D002273:Carcinogens; C531950:MIRN21 microRNA, mouse; C551848:MIRN34a microRNA, mouse; D035683:MicroRNAs; C532623:Mirn155 microRNA, mouse; D015127:9,10-Dimethyl-1,2-benzanthracene; D008770:Methylnitrosourea