title: Expression of the chimeric receptor between the chitin elicitor receptor CEBiP and the receptor-like protein kinase Pi-d2 leads to enhanced responses to the chitin elicitor and disease resistance against Magnaporthe oryzae in rice.
abstract: We previously reported that rice plants expressing the chimeric receptor consisting of rice chitin oligosaccharides binding protein (CEBiP) and the intracellular protein kinase region of Xa21, which confers resistance to rice bacterial blight, showed enhanced cellular responses to a chitin elicitor N-acetylchitoheptaose and increased resistance to the rice blast fungus Magnaporthe oryzae. Here, we investigated whether CEBiP fused with another type of receptor-like protein kinase (RLK) also functions as a chimeric receptor. Fusion proteins CRPis consist of CEBiP and the intracellular protein kinase region of a true resistance gene Pi-d2. Transgenic rice expressing a CRPi showed enhanced cellular responses specifically to N-acetylchitoheptaose in cultured cells and increased levels of disease resistance against M. oryzae in plants. These responses depended on the amino acid sequences predicted to be essential for the protein kinase activity of CRPi. The structure of the transmembrane domain in CRPi affected the protein accumulation, cellular responses, and disease resistance in transgenic rice. These results suggest that the chimeric receptor consisting of CEBiP and Pi-d2 functions as a receptor for chitin oligosaccharides and CEBiP-based chimeric receptors fused with other RLKs may also act as functional receptors.
authors.affiliation: Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kouzai
authors.forename: Hanae
authors.initials: H
authors.lastname: Kaku
authors.forename: Naoto
authors.initials: N
authors.lastname: Shibuya
authors.forename: Eiichi
authors.initials: E
authors.lastname: Minami
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nishizawa
mesh_terms: D000595:Amino Acid Sequence; D002478:Cells, Cultured; D002686:Chitin / Q000378:metabolism; D060467:Disease Resistance / Q000502:physiology*; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D054884:Host-Pathogen Interactions; D006861:Hydrogen Peroxide / Q000378:metabolism; D020082:Magnaporthe / Q000502:physiology*; D008969:Molecular Sequence Data; D009154:Mutation; D009844:Oligosaccharides / Q000378:metabolism; D012275:Oryza / Q000235:genetics / Q000276:immunology* / Q000382:microbiology / Q000502:physiology; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins; D015398:Signal Transduction / Q000502:physiology*; D019076:Transgenes
chemical_list: C514377:N-acetylchitoheptaose; D009844:Oligosaccharides; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D002686:Chitin; D006861:Hydrogen Peroxide