title: Feline mammary carcinoma stem cells are tumorigenic, radioresistant, chemoresistant and defective in activation of the ATM/p53 DNA damage pathway.
abstract: Cancer stem cells were identified in a feline mammary carcinoma cell line by demonstrating expression of CD133 and utilising the tumour sphere assay. A population of cells was identified that had an invasive, mesenchymal phenotype, expressed markers of pluripotency and enhanced tumour formation in the NOD-SCID mouse and chick embryo models. This population of feline mammary carcinoma stem cells was resistant to chemotherapy and radiation, possibly due to aberrant activation of the ATM/p53 DNA damage pathway. Epithelial-mesenchymal transition was a feature of the invasive phenotype. These data demonstrate that cancer stem cells are a feature of mammary cancer in cats.
authors.affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Edinburgh EH25 9RG, UK. lisa.pang@ed.ac.uk
authors.forename: L Y
authors.initials: LY
authors.lastname: Pang
authors.forename: T M
authors.initials: TM
authors.lastname: Blacking
authors.forename: R W
authors.initials: RW
authors.lastname: Else
authors.forename: A
authors.initials: A
authors.lastname: Sherman
authors.forename: H M
authors.initials: HM
authors.lastname: Sang
authors.forename: B A
authors.initials: BA
authors.lastname: Whitelaw
authors.forename: T R
authors.initials: TR
authors.lastname: Hupp
authors.forename: D J
authors.initials: DJ
authors.lastname: Argyle
mesh_terms: D000071916:AC133 Antigen; D000243:Adenosine Deaminase / Q000172:deficiency; D000361:Agammaglobulinemia; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology; D064007:Ataxia Telangiectasia Mutated Proteins / Q000235:genetics / Q000378:metabolism*; D002277:Carcinoma / Q000662:veterinary; D002371:Cat Diseases / Q000473:pathology*; D002415:Cats; D004249:DNA Damage; D019008:Drug Resistance, Neoplasm; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D015674:Mammary Neoplasms, Animal / Q000473:pathology*; D051379:Mice; D009374:Neoplasms, Experimental; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000473:pathology / Q000502:physiology* / Q000528:radiation effects; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D016511:Severe Combined Immunodeficiency; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D000970:Antineoplastic Agents; D006023:Glycoproteins; D010455:Peptides; C000606024:Prom1 protein, mouse; D016159:Tumor Suppressor Protein p53; D064007:Ataxia Telangiectasia Mutated Proteins; D000243:Adenosine Deaminase