title: Toxicity evaluation of chromium picolinate nanoparticles in vivo and in vitro in rat.
abstract: The toxicity of nanoCrpic is still not understood and needs further investigation. Thus, this study investigated the effect of chromium picolinate nanoparticles (nanoCrpic) on the toxicity in vivo and in vitro in rat. In the in vivo study, 36 rats (Wistar, 8-week-old) were randomly divided into the control group (fed basal diet), the low-dose (300 ppb, μg/kg), and high-dose (1,000 ppb) nanoCrpic groups. The trial was conducted for 2 months; at the final stage of the trial, the rats were sacrificed, liver and kidney were examined, and samples of tissues were taken for histological examination. Hepatocytes isolated from 10-week-old Wistar male rats were used for in vitro study to examine the degree of DNA damage following exposure to 0 and 0.294 mM of H(2)O(2) for 30 min. Incubation medium was supplemented with 0 (control), 100, and 300 ppb nanoCrpic. In vivo study indicated that no lesions of liver or kidney were detected in 300 and 1,000 ppb nanoCrpic fed rats. The in vitro study evaluated DNA damage according to the percentage and distance of the fragments migration and revealed that there was insignificant difference between the nanoCrpic and control groups (p > 0.05). This study indicated that nanoCrpic at 300-1,000 ppb in vivo and at 100-300 ppb in vitro showed no signs of toxicity to rats.
authors.affiliation: Department of Animal Science, National Chiayi University, Luh Liau Li, Chiayi, Taiwan, Republic of China.
authors.forename: Shih-Yi
authors.initials: SY
authors.lastname: Chen
authors.forename: Tu-Fa
authors.initials: TF
authors.lastname: Lien
mesh_terms: D000818:Animals; D003470:Culture Media / Q000737:chemistry; D004249:DNA Damage; D022781:Hepatocytes / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007668:Kidney / Q000473:pathology; D008099:Liver / Q000473:pathology; D008297:Male; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000008:administration & dosage / Q000633:toxicity*; D010316:Particle Size; D010848:Picolinic Acids / Q000008:administration & dosage / Q000633:toxicity*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D018675:Toxicity Tests / Q000379:methods*
chemical_list: D003470:Culture Media; D010848:Picolinic Acids; D006861:Hydrogen Peroxide; C030614:picolinic acid