title: Ultrasensitive detection of microRNAs using nanoengineered micro gold shells and laser desorption/ionization time-of-flight MS.
abstract: We report on a simple, fast, and highly sensitive method that allows femtomolar detection of microRNAs (miRNAs) without the need of polymerase chain reaction using a nanoengineered micro gold shell (μAuS) and laser desorption/ionization time-of-flight (LDI-TOF) mass spectrometry (MS). Target miRNAs are selectively captured by a chemically decorated gold chip and a μAuS, which carries with it a large number of small molecules, termed amplification tags (Am-tags). Subsequent LDI-TOF MS analysis allows a highly amplified mass signal of Am-tags for the presence of target miRNAs in a sample, resulting in ultrasensitive detection.
authors.affiliation: Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Hyunjung
authors.initials: H
authors.lastname: Seo
authors.forename: Sohyun
authors.initials: S
authors.lastname: Kim
authors.forename: Jae Il
authors.initials: JI
authors.lastname: Kim
authors.forename: Hyunook
authors.initials: H
authors.lastname: Kang
authors.forename: Woong
authors.initials: W
authors.lastname: Jung
authors.forename: Woon-Seok
authors.initials: WS
authors.lastname: Yeo
mesh_terms: D004247:DNA / Q000737:chemistry; D005033:Ethylmaleimide / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D035683:MicroRNAs / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D035683:MicroRNAs; D006046:Gold; D004247:DNA; D005033:Ethylmaleimide