title: Preotic neural crest cells contribute to coronary artery smooth muscle involving endothelin signalling.
abstract: Neural crest cells constitute a multipotent cell population that gives rise to diverse cell lineages. The neural crest arising from the postotic hindbrain is known as the 'cardiac' neural crest, and contributes to the great vessels and outflow tract endocardial cushions, but the neural crest contribution to structures within the heart remains largely controversial. Here we demonstrate that neural crest cells from the preotic region migrate into the heart and differentiate into coronary artery smooth muscle cells. Preotic neural crest cells preferentially distribute to the conotruncal region and interventricular septum. Ablation of the preotic neural crest causes abnormalities in coronary septal branch and orifice formation. Mice and chicks lacking endothelin signalling show similar abnormalities in the coronary artery, indicating its involvement in neural crest-dependent coronary artery formation. This is the first report that reveals the preotic neural crest contribution to heart development and smooth muscle heterogeneity within a coronary artery.
authors.affiliation: Department of Physiological Chemistry and Metabolism, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Arima
authors.forename: Sachiko
authors.initials: S
authors.lastname: Miyagawa-Tomita
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Maeda
authors.forename: Rieko
authors.initials: R
authors.lastname: Asai
authors.forename: Daiki
authors.initials: D
authors.lastname: Seya
authors.forename: Maryline
authors.initials: M
authors.lastname: Minoux
authors.forename: Filippo M
authors.initials: FM
authors.lastname: Rijli
authors.forename: Koichi
authors.initials: K
authors.lastname: Nishiyama
authors.forename: Ki-Sung
authors.initials: KS
authors.lastname: Kim
authors.forename: Yasunobu
authors.initials: Y
authors.lastname: Uchijima
authors.forename: Hisao
authors.initials: H
authors.lastname: Ogawa
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kurihara
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kurihara
mesh_terms: D000818:Animals; D002642:Chick Embryo; D003331:Coronary Vessels / Q000196:embryology* / Q000254:growth & development / Q000502:physiology; D003370:Coturnix / Q000196:embryology; D016232:Endothelins / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D018345:Mice, Knockout; D009131:Muscle, Smooth, Vascular / Q000196:embryology* / Q000254:growth & development / Q000502:physiology; D009432:Neural Crest / Q000166:cytology / Q000196:embryology* / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D016232:Endothelins