title: (-)-Epicatechin gallate prevents alkali-salt mediated fibrillogenesis of hen egg white lysozyme.
abstract: Green tea polyphenols (GTPs) are found to be potent inhibitors of amyloid fibril formation. We report the effective inhibitory property of (-)-epicatechin gallate (ECG) during the alkali-salt induced fibrillogenesis of hen egg white lysozyme (HEWL) at 37 °C. Spectroscopic techniques such as fluorescence, circular dichroism and microscopic images show that (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECG), and (-)-epigallocatechin gallate (EGCG) show moderate inhibition of fibrillation with ECG as the most potent polyphenol. Aromatic interactions, hydrophobic interactions, the radical scavenging activity and autoxidation of polyphenols are likely to be the major reasons for ECG being the most effective inhibitor.
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
authors.forename: Sudeshna
authors.initials: S
authors.lastname: Ghosh
authors.forename: Nitin K
authors.initials: NK
authors.lastname: Pandey
authors.forename: Swagata
authors.initials: S
authors.lastname: Dasgupta
mesh_terms: D000468:Alkalies / Q000494:pharmacology*; D000818:Animals; D052160:Benzothiazoles; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D002645:Chickens; D002942:Circular Dichroism; D006160:Guanosine Triphosphate / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008856:Microscopy, Fluorescence; D009113:Muramidase / Q000737:chemistry* / Q000648:ultrastructure; D009391:Nephelometry and Turbidimetry; D017433:Protein Structure, Secondary; D012492:Salts / Q000494:pharmacology*; D013844:Thiazoles / Q000378:metabolism; D013997:Time Factors; D014364:Tryptophan / Q000378:metabolism
chemical_list: D000468:Alkalies; D052160:Benzothiazoles; D012492:Salts; D013844:Thiazoles; C009462:thioflavin T; D006160:Guanosine Triphosphate; D014364:Tryptophan; D002392:Catechin; C062669:epicatechin gallate; C045651:epigallocatechin gallate; C421864:hen egg lysozyme; D009113:Muramidase; C057580:gallocatechol