title: The Arabidopsis LECTIN RECEPTOR KINASE-VI.2 is a functional protein kinase and is dispensable for basal resistance to Botrytis cinerea.
abstract: Sensing of microbial pathogens by pathogen-associated molecular patterns (PAMPs) through pattern recognition receptors (PRRs) elicits a defense program known as PAMP-triggered immunity (PTI). Recently, we have shown that the Arabidopsis thaliana L-TYPE LECTIN RECEPTOR KINASE-VI.2 (LecRK-VI.2) positively regulates bacterial PTI. In this report, we suggest by in silico analysis that the kinase domain of LecRK-VI.2 is functional. LecRK-VI.2 also demonstrated auto-phosphorylation activity in vitro in the presence of divalent metal cations indicating that LecRK-VI.2 has the ability to auto-phosphorylate. We further investigate the role of LecRK-VI.2 in Arabidopsis resistance to the necrotrophic fungal pathogen Botrytis cinerea. Disruption of LecRK-VI.2 did not affect Arabidopsis resistance to B. cinerea. Accordingly, wild-type upregulation levels of PTI-responsive WRKY53, FRK1, NHL10, CYP81F2 and CBP60 g after treatment with the fungal PAMP chitin were observed in lecrk-VI.2-1. These data provide evidences that the kinase domain of LecRK-VI.2 is active and show that LecRK-VI.2 is not critical for resistance to the fungal pathogen B. cinerea.
authors.affiliation: Department of Life Science and Institute of Plant Biology; National Taiwan University; Taipei, Taiwan.
authors.forename: Prashant
authors.initials: P
authors.lastname: Singh
authors.affiliation: Department of Life Science and Institute of Plant Biology; National Taiwan University; Taipei, Taiwan.
authors.forename: Chih-Cheng
authors.initials: CC
authors.lastname: Chien
authors.affiliation: Department of Life Science and Institute of Plant Biology; National Taiwan University; Taipei, Taiwan.
authors.forename: Swati
authors.initials: S
authors.lastname: Mishra
authors.affiliation: Department of Life Science and Institute of Plant Biology; National Taiwan University; Taipei, Taiwan.
authors.forename: Chia-Hong
authors.initials: CH
authors.lastname: Tsai
authors.affiliation: Department of Life Science and Institute of Plant Biology; National Taiwan University; Taipei, Taiwan.
authors.forename: Laurent
authors.initials: L
authors.lastname: Zimmerli
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D020171:Botrytis; D002686:Chitin; D060467:Disease Resistance; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D010766:Phosphorylation; D010935:Plant Diseases / Q000382:microbiology*; D011494:Protein Kinases / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D051192:Receptors, Pattern Recognition / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D029681:Arabidopsis Proteins; D051192:Receptors, Pattern Recognition; D002686:Chitin; D011494:Protein Kinases; C099543:ATHLECRK protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases