title: Ampicillin alone and in combination with riboflavin modulates Staphylococcus aureus infection induced septic arthritis in mice.
abstract: Effects of ampicillin (Amp) in combination with riboflavin on septic arthritis in mice infected with Staphylococcus aureus have been reported. Ampicillin was given at 100 mg/kg after 24 h of infection, followed by riboflavin (Ribo) at 20 mg/kg body wt, after 2 h of Amp treatment. Mice were sacrificed at 3, 9, 15 days post infection (dpi). Combined treatment of infected mice with ampicillin and riboflavin eradicated the bacteria from blood, spleen and synovial tissue and showed a significant gross reduction in arthritis, reduced serum levels of TNF-alpha and IFN-gamma. S. aureus infected mice exhibited higher synovial TNF-alpha and IL-6, which was also reduced by ampicillin and riboflavin treatment. S. aureus infected mice showed a disturbed antioxidant status measured in terms of cellular anti-oxidants like reduced glutathione and anti-oxidant enzymes such as superoxide dismutase and catalase and were ameliorated when the animals were co-treated with ampicillin along with riboflavin. Results of the study showed that combined treatment with anti-oxidant and antibiotic may protect from staphylococcal arthritis and may ameliorate oxidative stress caused by S. aureus infection.
authors.affiliation: Department of Physiology, Immunology Laboratory, University Colleges of Science and Technology, University of Calcutta, 92 APC Road, Kolkata 700 009, India.
authors.forename: Pinky
authors.initials: P
authors.lastname: Mal
authors.forename: Deboshree
authors.initials: D
authors.lastname: Ghosh
authors.forename: Debasish
authors.initials: D
authors.lastname: Bandyopadhyay
authors.forename: Kallol
authors.initials: K
authors.lastname: Dutta
authors.forename: Biswadev
authors.initials: B
authors.lastname: Bishayi
mesh_terms: D000667:Ampicillin / Q000627:therapeutic use*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D000975:Antioxidants / Q000378:metabolism; D001170:Arthritis, Infectious / Q000188:drug therapy* / Q000378:metabolism / Q000382:microbiology*; D015153:Blotting, Western; D002374:Catalase / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004359:Drug Therapy, Combination; D005978:Glutathione / Q000378:metabolism; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D017319:Photosensitizing Agents / Q000627:therapeutic use; D012256:Riboflavin / Q000627:therapeutic use*; D013154:Spleen / Q000378:metabolism / Q000382:microbiology; D013203:Staphylococcal Infections / Q000150:complications / Q000175:diagnosis* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000235:genetics / Q000472:pathogenicity*; D013482:Superoxide Dismutase / Q000378:metabolism; D013582:Synovial Fluid / Q000378:metabolism / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D016207:Cytokines; D004269:DNA, Bacterial; D017319:Photosensitizing Agents; D000667:Ampicillin; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D012256:Riboflavin