title: Structural elucidation of a novel mannogalactan isolated from the fruiting bodies of Pleurotus geesteranus.
abstract: A novel water-soluble polysaccharide named PGPB-1, with a molecular weight of 1.3 × 10(4)Da as determined by high-performance liquid chromatography (HPLC), was isolated from the fruiting bodies of Pleurotus geesteranus by boiling water as well as DEAE-Sepharose Fast Flow and High-Resolution Sephacryl S-300. On the bases of compositional analysis, methylation analysis and NMR spectroscopy ((1)H NMR, (13)C NMR, 2D-COSY, TOCSY, HMQC, HMBC and NOESY spectra) analysis, the structural elucidation of PGPB-1 consisted of a α-D-(1→6)-galactopyranan and a α-D-3-O-Me-D-galactosyl unit backbone with a α-D-Mannosyl unit on O-2 of the 2,6-di-O-substituted-D-galactosyl units. The repeating unit of the polysaccharide was established as: [formula in text] PGPB-1 also contains a minor proportion of 6-linked-D-Glcp.
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, People's Republic of China.
authors.forename: An-qiang
authors.initials: AQ
authors.lastname: Zhang
authors.forename: Mei
authors.initials: M
authors.lastname: Xu
authors.forename: Li
authors.initials: L
authors.lastname: Fu
authors.forename: Pei-long
authors.initials: PL
authors.lastname: Sun
mesh_terms: D005638:Fruit / Q000737:chemistry*; D005685:Galactans / Q000737:chemistry / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000737:chemistry*; D015394:Molecular Structure; D020076:Pleurotus / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D005685:Galactans; D011134:Polysaccharides; D008358:Mannose