title: [Analysis of fingerprint and bioactive components of Bidens biternata by HPLC].
abstract: OBJECTIVE
  To establish HPLC fingerprint of Bidens biternata from different habitats and determine the contents of hyperoside, isoquercetin, astragalin and bipinnatapolyacetylpside.
  
  
  METHODS
  Analysis was carried on Hypersil ODS C18 column (4.6 mm x 250 mm, 5.0 microm) with acetonitrile and 3% acetic acid as the mobile phase in a gradient elution. The contents of 4 components were determined simultaneously.
  
  
  RESULTS
  The fingerprint of 10 populations were established and the data were analyzed by the similarity evaluation software. There were almost no differences between the similarities of 10 population, but the contents of 4 main compoerls were different among them.
  
  
  CONCLUSIONS
  This method is stable and reliable which could be applied in quality assessment.
authors.affiliation: School of Pharmacy, Key Laboratory of State Administration of Traditional Chinese Medicine, Anhui Medical University, Hefei 230032, China. cynthia.tt@163.com
authors.forename: Tao-Tao
authors.initials: TT
authors.lastname: Ma
authors.forename: Jin
authors.initials: J
authors.lastname: Xie
authors.forename: Qun-Lin
authors.initials: QL
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Fei-Hu
authors.initials: FH
authors.lastname: Chen
mesh_terms: D031176:Bidens / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D017753:Ecosystem; D047309:Flavones / Q000032:analysis*; D044949:Kaempferols / Q000032:analysis; D035261:Plant Components, Aerial / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D047309:Flavones; D044949:Kaempferols; D012997:Solvents; C016527:isoquercitrin; C021304:hyperoside; D011794:Quercetin; C001579:astragalin