title: Downregulation of CDH13 expression promotes invasiveness of bladder transitional cell carcinoma.
abstract: OBJECTIVE
  To investigate the association of downregulated CDH13 expression with invasiveness of bladder transitional cell carcinoma (TCC).
  
  
  METHODS
  CDH13 and matrix metalloproteinase-2 (MMP2) expression was detected in 23 normal bladder epithelial tissues and 71 bladder TCC tissues. RNA interference was used to inhibit CDH13 expression in bladder TCC 5637 cells and then analyzed its effects on migration, invasion, adhesion, and proliferation of 5637 cells, as well as MMP2 expression in 5637 cells.
  
  
  RESULTS
  The CDH13 expression in bladder TCC tissues was significantly lower than that in normal bladder epithelial tissues. Moreover, the expression of CDH13 from the muscle-invasive group was significantly lower than that from the non-muscle-invasive group. In addition, the MMP2 expression was increased in bladder TCC, especially in muscle-invasive tumors. After the transfection of CDH13 siRNA into 5637 cells, CDH13 expression was significantly decreased, and the migration, invasion, adhesion of 5637 cells, as well as MMP2 expression in 5637 cells was significantly promoted compared with blank and negative controls.
  
  
  CONCLUSIONS
  Downregulated expression of CDH13 is associated with increased invasion of bladder TCC, and may be due to the enhancement of cell-extracellular matrix adhesion and increased MMP2 expression.
authors.affiliation: Department of Urology, Xuzhou Tumor Hospital (Xuzhou Third People's Hospital), Xuzhou, China.
authors.forename: Ying-Li
authors.initials: YL
authors.lastname: Lin
authors.forename: Zheng-Kai
authors.initials: ZK
authors.lastname: He
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Li
authors.forename: Tong-Yu
authors.initials: TY
authors.lastname: Guan
mesh_terms: D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002295:Carcinoma, Transitional Cell / Q000378:metabolism* / Q000473:pathology*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D014162:Transfection; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000473:pathology*
chemical_list: D015820:Cadherins; C099972:H-cadherin; D034741:RNA, Small Interfering; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2