title: Cyclin-dependent kinase E1 (CDKE1) provides a cellular switch in plants between growth and stress responses.
abstract: Plants must deal effectively with unfavorable growth conditions that necessitate a coordinated response to integrate cellular signals with mitochondrial retrograde signals. A genetic screen was carried out to identify regulators of alternative oxidase (rao mutants), using AOX1a expression as a model system to study retrograde signaling in plants. Two independent rao1 mutant alleles identified CDKE1 as a central nuclear component integrating mitochondrial retrograde signals with energy signals under stress. CDKE1 is also necessary for responses to general cellular stresses, such as H(2)O(2) and cold that act, at least in part, via anterograde pathways, and integrates signals from central energy/stress sensing kinase signal transduction pathways within the nucleus. Together, these results place CDKE1 as a central kinase integrating diverse cellular signals and shed light on a mechanism by which plants can effectively switch between growth and stress responses.
authors.affiliation: Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, 35 Stirling Highway, Crawley, Western Australia 6009, Australia.
authors.forename: Sophia
authors.initials: S
authors.lastname: Ng
authors.forename: Estelle
authors.initials: E
authors.lastname: Giraud
authors.forename: Owen
authors.initials: O
authors.lastname: Duncan
authors.forename: Simon R
authors.initials: SR
authors.lastname: Law
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.forename: Reena
authors.initials: R
authors.lastname: Narsai
authors.forename: Chris
authors.initials: C
authors.lastname: Carrie
authors.forename: Hayden
authors.initials: H
authors.lastname: Walker
authors.forename: David A
authors.initials: DA
authors.lastname: Day
authors.forename: Nicolás E
authors.initials: NE
authors.lastname: Blanco
authors.forename: Åsa
authors.initials: Å
authors.lastname: Strand
authors.forename: James
authors.initials: J
authors.lastname: Whelan
authors.forename: Aneta
authors.initials: A
authors.lastname: Ivanova
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018844:Cyclin-Dependent Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005453:Fluorescence; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant / Q000235:genetics; D024101:Mitochondrial Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010088:Oxidoreductases / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013312:Stress, Physiological; D014157:Transcription Factors / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics; D000099188:Alternative Oxidase; D017346:Protein Serine-Threonine Kinases; D056850:Cyclin-Dependent Kinase 8
chemical_list: D029681:Arabidopsis Proteins; D018844:Cyclin-Dependent Kinases; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010940:Plant Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D000099188:Alternative Oxidase; C531469:At3g01090 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases; C522174:AT5G63610 protein, Arabidopsis; D056850:Cyclin-Dependent Kinase 8