title: Biocompatibility of membranes with unrestricted somatic stem cells.
abstract: OBJECTIVE
  The biocompatibility of human osteoblasts (HOB) and human unrestricted somatic stem cells (USSCs) with membranes (BioGide®, GORE-TEX®, GENTA-FOIL resorb®, RESODONT®, BioMend®, BioMend® Extend™) was evaluated.
  
  
  METHODS
  After osteogenic differentiation (dexamethasone, ascorbic acid and β-glycerolphosphate) cells were seeded on membranes. On days 1, 3 and 7, attachment, proliferation, cell vitality, cytotoxicty and cell morphology were analyzed.
  
  
  RESULTS
  Cells on BioGide® and RESODONT® exhibited significantly higher attachment (p<0.005) and proliferation (p<0.005). On BioMend® cells showed a significantly higher attachment compared to BioMend® Extend™ (p<0.005), whereas on BioMend® Extend™ cells had significantly higher proliferation (p<0.005). The vitality of cells was significantly better on BioGide® and RESODONT® (p<0.005). There were no significant differences between USSCs and HOBs. Scanning electron microscopy confirmed these results.
  
  
  CONCLUSIONS
  BioGide® and RESODONT® had the best biocompatibility and are appropriate membranes for use in stem cell-derived regeneration of bone.
authors.affiliation: Department of Cranio- and Maxillofacial Surgery, Heinrich Heine University of Düsseldorf, Moorenstr. 5, 40225 Düsseldorf, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Naujoks
authors.forename: Felix Paulssen
authors.initials: FP
authors.lastname: Von Beck
authors.forename: Fabian
authors.initials: F
authors.lastname: Langenbach
authors.forename: Michael
authors.initials: M
authors.lastname: Hentschel
authors.forename: Karin
authors.initials: K
authors.lastname: Berr
authors.forename: Matthias
authors.initials: M
authors.lastname: Hofer
authors.forename: Rita
authors.initials: R
authors.lastname: Depprich
authors.forename: Norbert
authors.initials: N
authors.lastname: Kübler
authors.forename: Jörg
authors.initials: J
authors.lastname: Handschel
mesh_terms: D001205:Ascorbic Acid / Q000494:pharmacology; D001672:Biocompatible Materials / Q000592:standards*; D002448:Cell Adhesion; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D003094:Collagen / Q000592:standards; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D005994:Glycerophosphates / Q000494:pharmacology; D006801:Humans; D008297:Male; D008422:Materials Testing / Q000379:methods; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000648:ultrastructure; D010012:Osteogenesis / Q000187:drug effects; D011138:Polytetrafluoroethylene / Q000592:standards; D015203:Reproducibility of Results; D013234:Stem Cells / Q000166:cytology* / Q000648:ultrastructure
chemical_list: C106416:Bio-Gide; D001672:Biocompatible Materials; D005994:Glycerophosphates; D008567:Membranes, Artificial; D003907:Dexamethasone; D011138:Polytetrafluoroethylene; D003094:Collagen; D001205:Ascorbic Acid