title: Neuritin 1 promotes neuronal migration.
abstract: Neuritin 1 (Nrn1 or cpg15-1) is an activity-dependent protein involved in synaptic plasticity during brain development, a process that relies upon neuronal migration. By analyzing Nrn1 expression, we found that it is highly expressed in a mouse model of migrating immortalized neurons (GN11 cells), but not in a mouse model of non-migrating neurons (GT1-7 cells). We thus hypothesized that Nrn1 might control neuronal migration. By using complementary assays, as Boyden's microchemotaxis, scratch-wounding and live cell imaging, we found that GN11 cell migration is enhanced when Nrn1 is overexpressed and decreased when Nrn1 is silenced. The effects of Nrn1 in promoting neuronal migration have been then confirmed ex vivo, on rat cortical interneurons, by Boyden chamber assays and focal electroporation of acute embryonic brain slices. Furthermore, we found that Nrn1 level modulation affects GN11 cell morphology. The process is also paralleled by Nrn1-induced α-tubulin post-translational modifications, a well-recognized marker of microtubule stability. Altogether, the data demonstrate a novel function of Nrn1 in promoting migration of neuronal cells and indicate that Nrn1 levels impact on microtubule stability.
authors.affiliation: Dipartimento di Scienze Farmacologiche e Biomolecolari and Centre of Excellence on Neurodegenerative Diseases, Università degli Studi di Milano, Italia, Via Balzaretti, 9, 20133, Milan, Italy.
authors.forename: Arianna
authors.initials: A
authors.lastname: Zito
authors.forename: Daniele
authors.initials: D
authors.lastname: Cartelli
authors.forename: Graziella
authors.initials: G
authors.lastname: Cappelletti
authors.forename: Anna
authors.initials: A
authors.lastname: Cariboni
authors.forename: William
authors.initials: W
authors.lastname: Andrews
authors.forename: John
authors.initials: J
authors.lastname: Parnavelas
authors.forename: Angelo
authors.initials: A
authors.lastname: Poletti
authors.forename: Mariarita
authors.initials: M
authors.lastname: Galbiati
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D002454:Cell Differentiation; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D015536:Down-Regulation / Q000502:physiology; D004622:Embryo, Mammalian; D005260:Female; D058851:GPI-Linked Proteins / Q000235:genetics / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008473:Median Eminence / Q000166:cytology; D051379:Mice; D009474:Neurons / Q000502:physiology*; D009479:Neuropeptides / Q000235:genetics / Q000502:physiology*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D046509:Tissue Culture Techniques; D014404:Tubulin / Q000378:metabolism; D014947:Wounds and Injuries / Q000378:metabolism / Q000473:pathology
chemical_list: D058851:GPI-Linked Proteins; D009479:Neuropeptides; C105161:Nrn1 protein, rat; D014404:Tubulin; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins