title: A disposable immunosensor for Enterobacter sakazakii based on an electrochemically reduced graphene oxide-modified electrode.
abstract: A disposable immunosensor based on electrochemically reduced graphene oxide (ERGO) was developed for the detection of Enterobacter sakazakii. First, the graphene was deposited on a screen-printed carbon electrode (SPCE) by an electrochemical method. Second, the horseradish peroxidase-labeled bacteria-specific antibody was assembled onto the modified electrode to enhance the sensitivity of the immunosensor. The immunosensor constructed in this study can sensitively and rapidly detect E. sakazakii, and this method may contribute to further foodborne pathogen detection. In addition, this ERGO-modified SPCE could also provide new insights into the development of immunosensors for other bacteria to facilitate rapid detection.
authors.affiliation: Food Safety Key Lab of Zhejiang Province, Department of Food Quality and Safety, Zhejiang Gongshang University, Hangzhou 310035, People's Republic of China.
authors.forename: Xue
authors.initials: X
authors.lastname: Hu
authors.forename: Wenchao
authors.initials: W
authors.lastname: Dou
authors.forename: Linglin
authors.initials: L
authors.lastname: Fu
authors.forename: Guangying
authors.initials: G
authors.lastname: Zhao
mesh_terms: D044083:Cronobacter sakazakii; D004209:Disposable Equipment; D055664:Electrochemical Techniques; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D007118:Immunoassay / Q000295:instrumentation*; D018625:Microscopy, Atomic Force; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry*
chemical_list: D010087:Oxides; D006108:Graphite