title: Alpha B-crystallin is a new prognostic marker for laryngeal squamous cell carcinoma.
abstract: BACKGROUND
  Alpha B-crystallin (αB-crystallin) has been suggested to play an important role in the development of solid tumors. However, the association between αB-crystallin expression and clinicopathological characteristics of human laryngeal carcinoma is not well defined. This study aimed to examine the expression of αB-crystallin in human laryngeal squamous cell carcinoma (LSCC) and investigate the relationship between its expression and the prognosis of LSCC.
  
  
  METHODS
  Real-time polymerase chain reaction (six LSCC samples, six tumor-adjacent normal samples) and immunohistochemistry by tissue microarrays (109 LSCC samples and 28 tumor-adjacent normal samples) were performed to characterize expression of the αB-crystallin gene in LSCC. Kaplan-Meier survival and Cox regression analyses were carried out to evaluate the prognosis of LSCC.
  
  
  RESULTS
  Real-time polymerase chain reaction and immunohistochemistry analysis showed that the expression of αB-crystallin in LSCC was significantly higher than that in tumor-adjacent normal tissues. Moreover, the expression level of αB-crystallin protein in LSCC was significantly related to alcohol consumption (P = 0.022), tumor differentiation (P = 0.007), pTNM stage (P = 0.041) and 5 years' survival (P =0.030). COX multi-factor analysis showed that αB-crystallin (P = .013), as well as pTNM stage (P =0.027) and lymphatic metastasis (P = 0.015) were independent prognosis factors for LSCC.
  
  
  CONCLUSIONS
  The data suggest that αB-crystallin expression is correlated with malignant phenotypes of LSCC and it may serve as a novel prognostic factor for LSCC.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Jiangsu Province Official Hospital, No.65 Jiangsu Road, Nanjing 210029, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Mao
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Lin
authors.forename: Lin
authors.initials: L
authors.lastname: Xiong
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Qing-Dong
authors.initials: QD
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.forename: Qing
authors.initials: Q
authors.lastname: Cao
authors.forename: Ren-Jie
authors.initials: RJ
authors.lastname: Chen
authors.forename: Jin
authors.initials: J
authors.lastname: Zhu
authors.forename: Zhen-Qing
authors.initials: ZQ
authors.lastname: Feng
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D005260:Female; D015870:Gene Expression; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D038203:alpha-Crystallin B Chain / Q000235:genetics* / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D038203:alpha-Crystallin B Chain