title: Proteomic profile of edible bird's nest proteins.
abstract: Edible bird's nest (EBN) is made of the swiftlets' saliva, which has attracted rather more attention owing to its nutritious and medical properties. Although protein constitutes the main composition and plays an important role in EBN, few studies have focused on the proteomic profile of EBN. The purpose of this study was to produce a proteomic map and clarify common EBN proteins. Liquid-phase isoelectric focusing (LIEF) was combined with two-dimensional electrophoresis (2-DE) for comprehensive analysis of EBN proteins. From 20 to 100 protein spots were detected on 2-DE maps of EBN samples from 15 different sources. The proteins were mainly distributed in four taxa (A, B, C, and D) according to their molecular mass. Taxa A and D both contained common proteins and proteins that may be considered another characteristic of EBN. Taxon A was identified using MALDI-TOF-TOF/MS and found to be homologous to acidic mammalian chitinase-like ( Meleagris gallopavo ), which is in glycosyl hydrolase family 18.
authors.affiliation: Shenzhen Academy of Metrology and Quality Inspection , Shenzhen 518102, People's Republic of China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Liu
authors.forename: Xintian
authors.initials: X
authors.lastname: Lai
authors.forename: Shiwei
authors.initials: S
authors.lastname: Zhang
authors.forename: Xiuli
authors.initials: X
authors.lastname: Huang
authors.forename: Quanxue
authors.initials: Q
authors.lastname: Lan
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.forename: Bifang
authors.initials: B
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Qinlei
authors.initials: Q
authors.lastname: Zhang
authors.forename: Dezhi
authors.initials: D
authors.lastname: Hong
authors.forename: Guowu
authors.initials: G
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001717:Birds; D002688:Chitinases / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D007214:Indonesia; D008296:Malaysia; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics; D012463:Saliva / Q000737:chemistry*; D012471:Salivary Proteins and Peptides / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013785:Thailand; D014744:Vietnam
chemical_list: D011506:Proteins; D012471:Salivary Proteins and Peptides; D002688:Chitinases