title: Microscopic structural study of collagen aging in isolated fibrils using polarized second harmonic generation.
abstract: Polarization resolved second harmonic generation (PSHG) is developed to study, at the microscopic scale, the impact of aging on the structure of type I collagen fibrils in two-dimensional coatings. A ribose-glycated collagen is also used to mimic tissue glycation usually described as an indicator of aging. PSHG images are analyzed using a generic approach of the molecular disorder information in collagen fibrils, revealing significant changes upon aging, with a direct correlation between molecular disorder and fibril diameters.
authors.affiliation: Aix-Marseille Université, Institut Fresnel, CNRS UMR 7249, Ecole Centrale, Marseille, Domaine Universitaire St. Jérôme, 13013 Marseille, France.
authors.forename: Dora
authors.initials: D
authors.lastname: Aït-Belkacem
authors.forename: Marie
authors.initials: M
authors.lastname: Guilbert
authors.forename: Muriel
authors.initials: M
authors.lastname: Roche
authors.forename: Julien
authors.initials: J
authors.lastname: Duboisset
authors.forename: Patrick
authors.initials: P
authors.lastname: Ferrand
authors.forename: Ganesh
authors.initials: G
authors.lastname: Sockalingum
authors.forename: Pierre
authors.initials: P
authors.lastname: Jeannesson
authors.forename: Sophie
authors.initials: S
authors.lastname: Brasselet
mesh_terms: D000375:Aging / Q000473:pathology*; D024022:Fibrillar Collagens / Q000648:ultrastructure*; D008859:Microscopy, Polarization / Q000379:methods*; D011487:Protein Conformation
chemical_list: D024022:Fibrillar Collagens