title: Chronic resveratrol treatment protects pancreatic islets against oxidative stress in db/db mice.
abstract: Resveratrol (RSV) has anti-inflammatory and anti-oxidant actions which may contribute to its cardiovascular protective effects. We examined whether RSV has any beneficial effects on pancreatic islets in db/db mice, an animal model of type 2 diabetes. The db/db and db/dm mice (non-diabetic control) were treated with (db-RSV) or without RSV (db-control) (20 mg/kg daily) for 12 weeks. After performing an intraperitoneal glucose tolerance test and insulin tolerance test, mice were sacrificed, the pancreas was weighed, pancreatic β-cell mass was quantified by point count method, and the amount of islet fibrosis was determined. 8-Hydroxydeoxyguanosine (8-OHdG), an oxidative stress marker, was determined in 24 h urine and pancreatic islets. RSV treatment significantly improved glucose tolerance at 2 hrs in db/db mice (P = 0.036), but not in db/dm mice (P = 0.623). This was associated with a significant increase in both pancreas weight (P = 0.011) and β-cell mass (P = 0.016). Islet fibrosis was much less in RSV-treated mice (P = 0.048). RSV treatment also decreased urinary 8-OHdG levels (P = 0.03) and the percentage of islet nuclei that were positive for 8-OHdG immunostaining (P = 0.019). We conclude that RSV treatment improves glucose tolerance, attenuates β-cell loss, and reduces oxidative stress in type 2 diabetes. These findings suggest that RSV may have a therapeutic implication in the prevention and management of diabetes.
authors.affiliation: Division of Endocrinology and Metabolism, Department of Internal Medicine, The Catholic University of Korea, Seoul, Korea.
authors.forename: Young-Eun
authors.initials: YE
authors.lastname: Lee
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Kim
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Lee
authors.forename: Yu-Bae
authors.initials: YB
authors.lastname: Ahn
authors.forename: Ki-Ho
authors.initials: KH
authors.lastname: Song
authors.forename: Kun-Ho
authors.initials: KH
authors.lastname: Yoon
authors.forename: Hyung-Wook
authors.initials: HW
authors.lastname: Kim
authors.forename: Cheol-Whee
authors.initials: CW
authors.lastname: Park
authors.forename: Guolian
authors.initials: G
authors.lastname: Li
authors.forename: Zhenqi
authors.initials: Z
authors.lastname: Liu
authors.forename: Seung-Hyun
authors.initials: SH
authors.lastname: Ko
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001786:Blood Glucose / Q000032:analysis; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology*; D004195:Disease Models, Animal; D005355:Fibrosis / Q000517:prevention & control; D005951:Glucose Tolerance Test; D007150:Immunohistochemistry; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D007328:Insulin; D013267:Stilbenes; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D000077185:Resveratrol