title: Isolating lipid droplets from multiple species.
abstract: The lipid droplet (LD) is a cell organelle that has been linked to human metabolic syndromes and that can be exploited for the development of biofuels. The isolation of LDs is crucial for carrying out morphological and biochemical studies of this organelle. In the past two decades, LDs have been isolated from several organisms and investigated by microscopy, proteomics and lipidomics. However, these studies need to be extended to more model organisms, as well as to more animal tissues. Thus, a standard method that can be easily applied to these new samples with the need for minimal optimization is essential. Here we provide an LD isolation protocol that is relatively simple and suitable for a wide range of tissues and organisms. On the basis of previous studies, this 7-h protocol can yield 15-100 μg of protein-equivalent high-quality LDs that satisfy the requirements for current LD research in most organisms.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Ding
authors.forename: Shuyan
authors.initials: S
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Huimin
authors.initials: H
authors.lastname: Na
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Huina
authors.initials: H
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: Jinhai
authors.initials: J
authors.lastname: Yu
authors.forename: Chaoxing
authors.initials: C
authors.lastname: Huo
authors.forename: Shimeng
authors.initials: S
authors.lastname: Xu
authors.forename: Martina
authors.initials: M
authors.lastname: Garaiova
authors.forename: Yusheng
authors.initials: Y
authors.lastname: Cong
authors.forename: Pingsheng
authors.initials: P
authors.lastname: Liu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D016466:CHO Cells; D017173:Caenorhabditis elegans / Q000378:metabolism; D006224:Cricetinae; D008055:Lipids / Q000737:chemistry*; D008099:Liver / Q000378:metabolism; D051379:Mice; D015388:Organelles / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D008055:Lipids