title: Thioredoxin-1 was required for CREB activity by methamphetamine in rat pheochromocytoma cells.
abstract: Methamphetamine (METH) is one of the most commonly abused agents by illicit-drug users. Thioredoxin-1 (Trx-1) plays important biological roles both in intra- and extracellular compartments, including in regulation of various intracellular molecules via thiol redox control. In this study, we found that Trx-1 was induced by METH in rat pheochromocytoma PC12 cells. Furthermore, PI3K/Akt pathway was involved in METH-induced increase of Trx-1 expression. An increase in phosphorylated cAMP response element-binding protein (CREB) was also observed after exposure of PC12 cells to METH, which was inhibited by a PI3K inhibitor, LY294002. In addition, the siRNA targeted toTrx-1 reduced the level of phosphorylated CREB by METH, suggesting Trx-1 is necessary for increased activity of CREB by METH. The results obtained in this study showed that Trx-1 might play a role in the actions of METH.
authors.affiliation: Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, 650500, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Lv
authors.forename: Sheng-Dong
authors.initials: SD
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Bai
mesh_terms: D000310:Adrenal Gland Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002867:Chromones / Q000494:pharmacology; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D008694:Methamphetamine / Q000494:pharmacology*; D009025:Morpholines / Q000494:pharmacology; D016716:PC12 Cells; D010673:Pheochromocytoma / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects; D013879:Thioredoxins / Q000378:metabolism*
chemical_list: D002867:Chromones; D017362:Cyclic AMP Response Element-Binding Protein; D009025:Morpholines; D034741:RNA, Small Interfering; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; D008694:Methamphetamine; D013879:Thioredoxins; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt