title: Bacterial classification of fish-pathogenic Mycobacterium species by multigene phylogenetic analyses and MALDI Biotyper identification system.
abstract: Mycobacterium marinum is difficult to distinguish from other species of Mycobacterium isolated from fish using biochemical methods. Here, we used genetic and proteomic analyses to distinguish three Mycobacterium strains: M. marinum strains MB2 and Europe were isolated from tropical and marine fish in Thailand and Europe, and Mycobacterium sp. 012931 strain was isolated from yellowtail in Japan. In phylogenetic trees based on gyrB, rpoB, and Ag85B genes, Mycobacterium sp. 012931 clustered with M. marinum strains MB2 and Europe, but in trees based on 16S rRNA, hsp65, and Ag85A genes Mycobacterium sp. 012931 did not cluster with the other strains. In proteomic analyses using a Bruker matrix-assisted laser desorption ionization Biotyper, the mass profile of Mycobacterium sp. 012931 differed from the mass profiles of the other two fish M. marinum strains. Therefore, Mycobacterium sp. 012931 is similar to M. marinum but is not the same, suggesting that it could be a subspecies of M. marinum.
authors.affiliation: Animal Health Department of Research and Development Agricultural and Veterinary Division, Meiji Seika Pharma, 2-4-16, Kyobashi, Chuo-ku, Tokyo, 104-8002, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Kurokawa
authors.forename: Jun
authors.initials: J
authors.lastname: Kabayama
authors.forename: Tsuguaki
authors.initials: T
authors.lastname: Fukuyasu
authors.forename: Seong Don
authors.initials: SD
authors.lastname: Hwang
authors.forename: Chan-Il
authors.initials: CI
authors.lastname: Park
authors.forename: Seong-Bin
authors.initials: SB
authors.lastname: Park
authors.forename: Carmelo S
authors.initials: CS
authors.lastname: del Castillo
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Hikima
authors.forename: Tae-Sung
authors.initials: TS
authors.lastname: Jung
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Kondo
authors.forename: Ikuo
authors.initials: I
authors.lastname: Hirono
authors.forename: Haruko
authors.initials: H
authors.lastname: Takeyama
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D005060:Europe; D005399:Fishes / Q000382:microbiology*; D005798:Genes, Bacterial / Q000235:genetics*; D007564:Japan; D008969:Molecular Sequence Data; D019910:Mycobacterium marinum / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013785:Thailand
chemical_list: D017931:DNA Primers