title: In vitro and in vivo efficacy of a hydrated sodium calcium aluminosilicate to bind and reduce aflatoxin residues in tissues of broiler chicks fed aflatoxin B1.
abstract: The aim of this study was to determine the binding capacity of a hydrated sodium calcium aluminosilicate (HSCAS) for aflatoxin B(1) (AFB(1)), and the efficacy of the HSCAS to reduce the concentrations of residual AFB(1) and its metabolites in the liver and kidney of broilers fed AFB(1). One hundred 1-d-old male broilers (Ross 708) were maintained in chick batteries and allowed ad libitum access to feed and water. A completely randomized design was used with 5 replicate pens of 5 chicks assigned to each of 4 dietary treatments from hatch to 21 d. Dietary treatments included the following: A) basal diet (BD), with no HSCAS or AFB(1), B) BD supplemented with 0.5% HSCAS only, C) BD supplemented with 2.5 mg of AFB(1)/kg of feed, and D) BD supplemented with 2.5 mg of AFB(1)/kg of feed and 0.5% HSCAS. On d 21, 5 chicks from each treatment were anesthetized with carbon dioxide, killed by cervical dislocation, and samples of liver and kidney were collected for analysis of AFB(1) residues. The percentage of AFB(1) bound for each concentration of adsorbent (100, 10, 1, 0.5, 0.25, and 0.05 mg/10 mL) was 100, 91.1, 81.8, 75.4, 40.1, and 8.8%, respectively. Concentrations of aflatoxin residues (AFB(1), aflatoxicol, aflatoxins B(2) and G(1)) were lower (P < 0.05) in livers and kidneys of birds fed AFB(1) plus HSCAS (diet D), when compared with birds fed AFB(1) alone (diet C). However, histopathology data from the in vivo study indicated that HSCAS did not prevent lesions associated with aflatoxicosis. The decrease in the bioavailability of AFB(1) caused by the HSCAS reduced aflatoxin residues in liver and kidney, but not enough to completely prevent the toxic effects of AFB(1) in broilers.
authors.affiliation: Department of Food Engineering, School of Animal Science and Food Engineering, University of São Paulo, Pirassununga, SP, 13630-000, Brazil.
authors.forename: D V
authors.initials: DV
authors.lastname: Neeff
authors.forename: D R
authors.initials: DR
authors.lastname: Ledoux
authors.forename: G E
authors.initials: GE
authors.lastname: Rottinghaus
authors.forename: A J
authors.initials: AJ
authors.lastname: Bermudez
authors.forename: A
authors.initials: A
authors.lastname: Dakovic
authors.forename: R A
authors.initials: RA
authors.lastname: Murarolli
authors.forename: C A F
authors.initials: CA
authors.lastname: Oliveira
mesh_terms: D016604:Aflatoxin B1 / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity*; D000538:Aluminum Silicates / Q000737:chemistry*; D000821:Animal Feed; D000818:Animals; D002645:Chickens / Q000254:growth & development*; D004032:Diet / Q000662:veterinary; D004350:Drug Residues; D005506:Food Contamination / Q000517:prevention & control*; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000473:pathology; D009929:Organ Size / Q000187:drug effects; D011201:Poultry Diseases / Q000139:chemically induced / Q000517:prevention & control
chemical_list: D000538:Aluminum Silicates; C087965:sodium calcium aluminosilicate, hydrated; D016604:Aflatoxin B1