title: Hepatoprotective efficacy of Sharbat-e-Deenar against carbon tetrachloride-induced liver damage.
abstract: This study was undertaken to evaluate the antioxidant and hepatoprotective efficacy of Sharbat-e-Deenar (SD) against carbon tetrachloride (CCl(4))-induced hepatic damage in a rat model. The antioxidant activity of SD was estimated by DPPH assay, H(2)O(2) assay, and total phenolic contents. SD therapy at doses of 1, 2, and 4 mL/kg, orally, was administered after CCl(4) intoxication (1.5 mL/kg, intraperitoneally for 48 hours) in experimental animals. Hexobarbitone sleep time and bromosulfophthalein retention time also were determined against CCl(4)-induced liver damage. Exposure to CCl(4) in experimental animals showed biochemical and histopathological deterioration in the liver. Treatment with SD showed a marked protection on biochemical parameters, that is, alanine transaminase, aspartate transaminase, albumin, and urea. SD therapy exhibited a protective effect by restoring the level of lipid peroxidation-reduced glutathione, adenosine triphosphate, and glucose-6-phosphatase (G-6-Pase). Treatment with SD significantly recovered the level of hexobarbitone sleep time and bromosulfophthalein retention time. DPPH and H(2)O(2) assays showed antioxidant properties in a dose-dependent manner. Histopathological observations of liver sections showed recovery in liver architecture after SD treatment. These results indicate that SD exerts a protective effect on CCl(4)-induced hepatotoxicity, which may be due to its antioxidant properties.
authors.affiliation: School of Sciences, Biochemistry, Indira Gandhi National Open University, New Delhi, India. arvind.kumar@ignou.ac.in
authors.forename: Arvind Kumar
authors.initials: AK
authors.lastname: Shakya
authors.forename: Monika
authors.initials: M
authors.lastname: Saxena
authors.forename: Neetu
authors.initials: N
authors.lastname: Sharma
authors.forename: Sadhana
authors.initials: S
authors.lastname: Shrivastava
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Shukla
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002251:Carbon Tetrachloride / Q000009:adverse effects* / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000517:prevention & control*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D008099:Liver / Q000201:enzymology / Q000473:pathology; D019367:Medicine, Unani; D051379:Mice; D010946:Plants, Medicinal; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D002251:Carbon Tetrachloride