title: Chemoenzymatic synthesis of mono- and di-fluorinated Thomsen-Friedenreich (T) antigens and their sialylated derivatives.
abstract: Fluorinated Thomsen-Friedenreich (T) antigens were synthesized efficiently from chemically produced fluorinated monosaccharides using a highly efficient one-pot two-enzyme chemoenzymatic approach containing a galactokinase and a D-galactosyl-β1-3-N-acetyl-D-hexosamine phosphorylase. These fluorinated T-antigens were further sialylated to form fluorinated ST-antigens using a one-pot two-enzyme system containing a CMP-sialic acid synthetase and an α-2-3-sialyltransferase.
authors.affiliation: National Glycoengineering Research Center, School of Pharmaceutical Science, Shandong University, Jinan 250012, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Fengshan
authors.initials: F
authors.lastname: Wang
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Cao
mesh_terms: D015295:Antigens, Tumor-Associated, Carbohydrate / Q000737:chemistry* / Q000378:metabolism*; D000957:Antigens, Viral, Tumor / Q000378:metabolism; D002240:Carbohydrate Sequence; D054879:Halogenation; D006801:Humans; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry* / Q000378:metabolism*; D000216:N-Acylneuraminate Cytidylyltransferase / Q000378:metabolism; D009345:Neisseria meningitidis / Q000201:enzymology; D016979:Pasteurella multocida / Q000201:enzymology; D012799:Sialyltransferases / Q000378:metabolism; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D000957:Antigens, Viral, Tumor; D009077:Mucins; C087769:sialosyl-T antigen; C016467:Thomsen-Friedenreich antigen; D012799:Sialyltransferases; D000216:N-Acylneuraminate Cytidylyltransferase; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase