title: Processing of cellulose synthase (AcsAB) from Gluconacetobacter hansenii 23769.
abstract: The cellulose synthase protein (AcsAB) is encoded by a single gene in Gluconacetobacter hansenii ATCC 23769. We have examined the processing pattern of this enzyme and the localization of the cleavage products by heterologously expressing the truncated portions of the AcsAB protein and using specific antibodies generated against these regions. We found that the AcsAB protein is processed into three polypeptide subunits of molecular masses 46kDa, 34kDa and 95kDa. The 46kDa polypeptide (AcsA(cat)) harbors the conserved glycosyltransferase domain and hence contains the catalytic subunit of the enzyme. This polypeptide is localized in the cytoplasmic membrane. The 34kDa polypeptide (AcsA(reg)) is the regulatory subunit with the cyclic diGMP-binding PilZ domain. This polypeptide is largely cytoplasmic. The 95kDa subunit (AcsB) is of unknown function and contains a predicted signal peptide at its N-terminus. This subunit is localized in the outer membrane. In addition to this, we have also localized the AcsC protein in the outer membrane, confirming its predicted localization based on the OM-signal sequence at its N-terminus.
authors.affiliation: Department of Biochemistry and Molecular Biology, 305 South Frear, University Park, The Pennsylvania State University, PA 16802, USA.
authors.forename: Prashanti R
authors.initials: PR
authors.lastname: Iyer
authors.forename: Yu-An
authors.initials: YA
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Deng
authors.forename: John B
authors.initials: JB
authors.lastname: McManus
authors.forename: Teh-Hui
authors.initials: TH
authors.lastname: Kao
authors.forename: Ming
authors.initials: M
authors.lastname: Tien
mesh_terms: D041821:Gluconacetobacter / Q000145:classification / Q000201:enzymology* / Q000648:ultrastructure*; D005964:Glucosyltransferases / Q000096:biosynthesis* / Q000737:chemistry*; D013045:Species Specificity; D013347:Subcellular Fractions / Q000737:chemistry* / Q000201:enzymology*
chemical_list: D005964:Glucosyltransferases; C478648:cellulose synthase