title: [Development of SYBR Green I real-time RT-PCR for the detection of Ebola virus].
abstract: In order to establish a rapid and accurate method for the detection of Ebola virus (EBOV), the primers used in SYBR Green I real-time RT-PCR were designed based on the EBOV NP gene sequences published in GenBank. The SYBR Green I real-time RT-PCR was established and optimized for the detection of EBOV. The EBOV RNA that was transcribed in vitro was used as a template. The sensitivity of this method was found to reach 1.0 x 10(2) copies/microL and the detection range was 10(2) - 10(10). No cross reaction with RNA samples from Marburg virus, Dengue virus, Xinjiang hemorrhagic fever virus, Japanese encephalitis virus, Influenza virus (H1N1 and H3N2) and Porcine reproductive and respiratory syndrome virus E genomic RNA was found. The method would be useful for the detection and monitoring of EBOV in China.
authors.affiliation: Department of Veterinary Public Health, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Zi-Xue
authors.initials: ZX
authors.lastname: Shi
authors.forename: Yu-Kun
authors.initials: YK
authors.lastname: Ma
authors.forename: Hao-Ting
authors.initials: HT
authors.lastname: Wang
authors.forename: Zong-Yao
authors.initials: ZY
authors.lastname: Wang
authors.forename: Dong-Hua
authors.initials: DH
authors.lastname: Shao
authors.forename: Jian-Chao
authors.initials: JC
authors.lastname: Wei
authors.forename: Shao-Hui
authors.initials: SH
authors.lastname: Wang
authors.forename: Bei-Bei
authors.initials: BB
authors.lastname: Li
authors.forename: Shui-Ming
authors.initials: SM
authors.lastname: Wang
authors.forename: Xue-Hui
authors.initials: XH
authors.lastname: Liu
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Ma
mesh_terms: D052160:Benzothiazoles; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D003959:Diamines; D029043:Ebolavirus / Q000235:genetics / Q000302:isolation & purification*; D019142:Hemorrhagic Fever, Ebola / Q000821:virology*; D006801:Humans; D009930:Organic Chemicals / Q000737:chemistry; D011804:Quinolines; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D052160:Benzothiazoles; D017931:DNA Primers; D003959:Diamines; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I