title: [Effect of TGRJ on blood press, NO and Ang II in renal hypertensive rats].
abstract: OBJECTIVE
  To study the effect of total glycosides of Ranunculus japoniucus (TGRJ) on blood pressure, nitric oxide (NO) calcium and angiotensin II (Ang II) in renal hypertensive rats (RHR).
  
  
  METHODS
  1) RHR were established by two kidney one clip (2K1C) and drugs were given by intragastric administration for 5 week, the blood pressure were measured at the end of 5 week,detected the concentration of NO in the serum and Ang II in the blood plasma,heart and kidney tissue. 2) Used a new generations of Ca2+ fluorescent probe (Fluo-3/AM) to mark calcium in vascular smooth muscle cells. Observed the fluorescence imaging by inverted fluorescence microscope and measured the fluorescence intensity of calcium by fluorescence spectrophotometer in vascular smooth muscle cells.
  
  
  RESULTS
  1) The high, medium doses of TGRJ could decrease blood pressure of RHR (P<0.05), TGRJ could significantly increase the concentration of NO in the serum (P<0.01), but it showed no significant effect on the concentration of Ang II (P>0.05); 2) The Ang II could significantly promote the calcium ions in extracellular inner flow. The different doses of TGRJ could reduce the calcium ions in cells which were mediated by Ang II.
  
  
  CONCLUSIONS
  TGRJ could decrease blood pressure in RHR. the mechanism might be related to increasing the rats' NO level and reducing the calcium ions level in cells which are increased by Ang II.
authors.affiliation: Department of Pharmacology, Guangdong Pharmacy University, Guangzhou 510006, China. liushoujia@126.com
authors.forename: Shou-Jia
authors.initials: SJ
authors.lastname: Liu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhi-Cheng
authors.initials: ZC
authors.lastname: Yang
authors.forename: Wei-Zhen
authors.initials: WZ
authors.lastname: Liu
authors.forename: Yu-Zhi
authors.initials: YZ
authors.lastname: Tan
mesh_terms: D000804:Angiotensin II / Q000097:blood* / Q000378:metabolism; D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005260:Female; D006027:Glycosides / Q000494:pharmacology*; D006977:Hypertension, Renal / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000378:metabolism; D009569:Nitric Oxide / Q000097:blood*; D031947:Ranunculus / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000959:Antihypertensive Agents; D004365:Drugs, Chinese Herbal; D006027:Glycosides; D000804:Angiotensin II; D009569:Nitric Oxide; D002118:Calcium