title: Effects of waterborne chronic copper exposure on hepatic lipid metabolism and metal-element composition in Synechogobius hasta.
abstract: The present study was conducted to determine hepatic lipid metabolism and metal-element composition in Synechogobius hasta exposed to waterborne chronic copper (Cu) concentrations of control, 57, and 118 μg Cu/l, respectively, for 30 days. Growth decreased, but hepatosomatic index, viscerosomatic index, and hepatic lipid content increased with increasing waterborne Cu levels. Staining with oil red O showed extensive steatosis in liver of Cu-exposed fish. Cu exposure increased hepatic 6-phosphogluconate dehydrogenase, glucose-6-phosphate dehydrogenase, and malic enzyme activities, whereas fatty acid synthetase, isocitrate dehydrogenase, and carnitine palmitoyltransferases I activities remained unaffected. Cu, zinc, iron, and manganese contents were also changed in several tissues (gill, liver, spleen, gastrointestinal tract, and muscle) in a tissue-, dose-, and time-dependent manner. This was the first study to examine the effects of waterborne Cu exposure on several enzymatic activities mediating hepatic lipogenesis and lipolysis in fish as well as to show that waterborne Cu exposure could enhance the metabolism of lipid synthesis and consequently induce the increase of hepatic lipid deposition in S. hasta.
authors.affiliation: Fishery College, Huazhong Agricultural University, Wuhan, China.
authors.forename: Qi-Liang
authors.initials: QL
authors.lastname: Chen
authors.forename: Zhi
authors.initials: Z
authors.lastname: Luo
authors.forename: Xu
authors.initials: X
authors.lastname: Liu
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Song
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Liu
authors.forename: Jia-Lang
authors.initials: JL
authors.lastname: Zheng
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Zhao
mesh_terms: D000818:Animals; D003300:Copper / Q000633:toxicity*; D005942:Gluconates / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D010473:Perciformes / Q000502:physiology*; D036401:Toxicity Tests, Chronic; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D005942:Gluconates; D014874:Water Pollutants, Chemical; D003300:Copper; D005954:Glucosephosphate Dehydrogenase; C008884:6-phosphogluconic acid