title: Emaravirus-specific degenerate PCR primers allowed the identification of partial RNA-dependent RNA polymerase sequences of Maize red stripe virus and Pigeonpea sterility mosaic virus.
abstract: Emaravirus is a recently established viral genus that includes two approved virus species: European mountain ash ringspot-associated virus (EMARaV) and Fig mosaic virus (FMV). Other described but unclassified viruses appear to share biological characteristics similar to emaraviruses, including segmented, negative-single stranded RNA genomes with enveloped virions approximately 80-200nm in diameter. Sequence analysis of emaravirus genomes revealed the presence of conserved amino acid sequences in the RNA-dependent RNA polymerase gene (RdRp) denoted as pre-motif A, motifs A and C. Degenerate oligonucleotide primers were developed to these conserved sequences and were shown to amplify in reverse transcription-polymerase chain reaction assay (RT-PCR) DNA fragments of 276bp and 360bp in size. These primers efficiently detected emaraviruses with known sequences available in the database (FMV and EMARaV); they also detected viruses with limited sequence information such as Pigeonpea sterility mosaic virus (PPSMV) and Maize red stripe virus (MRSV). The degenerate primers designed on pre-motif A and motif A sequences successfully amplified the four species used as positive controls (276bp), whereas those of motifs A and C failed to detect only MRSV. The amino acid sequences obtained from PPSMV and MRSV shared the highest identity with those of two other tentative species of the Emaravirus genus, Rose rosette virus (RRV) (69%) and Redbud yellow ringspot virus (RYRV) (60%), respectively. The phylogenetic tree constructed with 92 amino acid-long portions of polypeptide putatively encoded by RNA1 of definitive and tentative emaravirus species clustered PPSMV and MRSV in two separate clades close to RRV and Raspberry leaf blotch virus (RLBV), respectively. The newly developed degenerate primers have proved their efficacy in amplifying new emaravirus-specific sequences; accordingly, they could be useful in identifying new emaravirus-like species in nature.
authors.affiliation: Istituto Agronomico Mediterraneo di Bari, Via Ceglie 9, 70010 Valenzano, Bari, Italy. elbeaino@iamb.it
authors.forename: Toufic
authors.initials: T
authors.lastname: Elbeaino
authors.forename: Anna
authors.initials: A
authors.lastname: Whitfield
authors.forename: Mamta
authors.initials: M
authors.lastname: Sharma
authors.forename: Michele
authors.initials: M
authors.lastname: Digiaro
mesh_terms: D000595:Amino Acid Sequence; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics*; D030681:Ficus; D010802:Phylogeny; D010942:Plant Viruses / Q000201:enzymology / Q000235:genetics*; D012328:RNA Viruses / Q000201:enzymology / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014713:Viruses, Unclassified / Q000201:enzymology / Q000235:genetics*; D003313:Zea mays
chemical_list: D017931:DNA Primers; D012324:RNA-Dependent RNA Polymerase