title: Biomass-to-bio-products application of feruloyl esterase from Aspergillus clavatus.
abstract: The structural polysaccharides contained in plant cell walls have been pointed to as a promising renewable alternative to petroleum and natural gas. Ferulic acid is a ubiquitous component of plant polysaccharides, which is found in either monomeric or dimeric forms and is covalently linked to arabinosyl residues. Ferulic acid has several commercial applications in food and pharmaceutical industries. The study herein introduces a novel feruloyl esterase from Aspergillus clavatus (AcFAE). Along with a comprehensive functional and biophysical characterization, the low-resolution structure of this enzyme was also determined by small-angle X-ray scattering. In addition, we described the production of phenolic compounds with antioxidant capacity from wheat arabinoxylan and sugarcane bagasse using AcFAE. The ability to specifically cleave ester linkages in hemicellulose is useful in several biotechnological applications, including improved accessibility to lignocellulosic enzymes for biofuel production.
authors.affiliation: Laboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Rua Giuseppe Máximo Scolfaro, no. 10.000, Caixa, Postal 6170 13083-970, Campinas, São Paulo, Brazil.
authors.forename: André R L
authors.initials: AR
authors.lastname: Damásio
authors.forename: Cleiton Márcio Pinto
authors.initials: CM
authors.lastname: Braga
authors.forename: Lívia B
authors.initials: LB
authors.lastname: Brenelli
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Citadini
authors.forename: Fernanda
authors.initials: F
authors.lastname: Mandelli
authors.forename: Junio
authors.initials: J
authors.lastname: Cota
authors.forename: Rodrigo Ferreira
authors.initials: RF
authors.lastname: de Almeida
authors.forename: Victor Hugo
authors.initials: VH
authors.lastname: Salvador
authors.forename: Douglas Antonio Alvaredo
authors.initials: DA
authors.lastname: Paixao
authors.forename: Fernando
authors.initials: F
authors.lastname: Segato
authors.forename: Adriana Zerlotti
authors.initials: AZ
authors.lastname: Mercadante
authors.forename: Mario
authors.initials: M
authors.lastname: de Oliveira Neto
authors.forename: Wanderley Dantas
authors.initials: WD
authors.lastname: do Santos
authors.forename: Fabio M
authors.initials: FM
authors.lastname: Squina
mesh_terms: D001230:Aspergillus / Q000201:enzymology*; D001483:Base Sequence; D018533:Biomass; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D017931:DNA Primers
chemical_list: D017931:DNA Primers; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase