title: [Effects of dexamethasone on IL-13-induced mCLCA3 and Muc5ac expressions in nasal mucosa of rats].
abstract: OBJECTIVE
  To study the effects of dexamethasone on the IL-13-induced mCLCA3 and Muc5ac expressions in nasal mucosa of rats,  and the role in mucus secretion of allergic rhinitis.
  
  
  METHODS
  Thirty SD rats were randomly divided into control group,  IL-13 group and dexamethasone group. Expressions of mCLCA3 mRNA and Muc5ac protein in nasal mucosa were detected by RT-PCR and immunohistochemistry,  respectively.
  
  
  RESULTS
  No mCLCA3 mRNA expression in the nasal mucosa was detected in the control group,  while it was 0.319±0.121 in the IL-13 group (P<0.05 vs control group) and 0.144±0.105 in the dexamethasone group (P<0.05 vs IL-13 group). The expression of Muc5ac protein increased in the IL-13 group (1.389±0.499) as compared with the control group (0.300±0.145,  P<0.05). After treatment with dexamethasone,  the expression of Muc5ac protein was notably lower (0.901±0.390) than that in IL-13 group (P<0.05).
  
  
  CONCLUSIONS
  IL-13 up-regulates the expressions of mCLCA3 mRNA and Muc5ac protein in nasal mucosa,  which may play a pivotal role in the mucus overproduction of nasal mucosa. Dexamethasone substantially down-regulates the expressions of mCLCA3 mRNA and Muc5ac protein,  which may have an inhibiting effect on mucus overproduction of nasal mucosa.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery,  Tangdu Hospital,  Fourth Military Medical University,  Xi'an 710038,  China.
authors.forename: Wen-li
authors.initials: WL
authors.lastname: Wu
authors.forename: Zhi
authors.initials: Z
authors.lastname: Liu
authors.forename: Li-mei
authors.initials: LM
authors.lastname: Zhong
authors.forename: Peng-cheng
authors.initials: PC
authors.lastname: Cui
authors.forename: Peng-fei
authors.initials: PF
authors.lastname: Gao
mesh_terms: D000818:Animals; D018118:Chloride Channels / Q000235:genetics* / Q000378:metabolism; D003907:Dexamethasone / Q000008:administration & dosage*; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D018793:Interleukin-13 / Q000235:genetics* / Q000378:metabolism; D055271:Mucin 5AC / Q000235:genetics* / Q000378:metabolism; D009297:Nasal Mucosa / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018118:Chloride Channels; D018793:Interleukin-13; C524680:Muc5ac protein, rat; D055271:Mucin 5AC; D003907:Dexamethasone