title: Variability of the fimA gene in Porphyromonas gingivalis  isolated from periodontitis and non-periodontitis patients.
abstract: OBJECTIVE
  The goal of this study was to determine the genetic variability of the fimA gene in Porphyromonas gingivalis isolates from Spanish patients.
  
  
  METHODS
  Pooled subgingival samples were taken, processed and cultured in non-selective blood agar medium. Pure cultures of one to six isolates per patient were obtained and PCR and PCR-RFLP were used for fimbrillin gene (fimA) type determination of the extracted genomic (DNA).
  
  
  RESULTS
  Two hundred and twenty four Porphyromonas gingivalis isolates from 65 patients were analyzed consisting of 15 non-periodontitis patients (66 isolates) and 50 with periodontitis (158 isolates). Genotype II was the most prevalent (50.9%), while the other types of fimbriae did not exceed fifteen percent of prevalence. Isolates with types II and IV of fimbriae were significantly more prevalent in periodontitis patients than isolates with genotype I. Co-infection was observed in 17.65% of the patients analyzed.
  
  
  CONCLUSIONS
  The results suggest that in this population Porphyromonas gingivalis with type II of fimbriae are significantly more predominant in periodontitis patients than genotype I.
authors.affiliation: ETEP Research Group, Complutense University, Madrid, Spain. simonefabrizi@gmail.com
authors.forename: Simone
authors.initials: S
authors.lastname: Fabrizi
authors.forename: Rubén
authors.initials: R
authors.lastname: León
authors.forename: Vanessa
authors.initials: V
authors.lastname: Blanc
authors.forename: David
authors.initials: D
authors.lastname: Herrera
authors.forename: Mariano
authors.initials: M
authors.lastname: Sanz
mesh_terms: D016866:Bacteroidaceae Infections; D036781:Fimbriae Proteins / Q000235:genetics*; D006801:Humans; D010518:Periodontitis / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000235:genetics* / Q000302:isolation & purification
chemical_list: C063055:fimbrillin; D036781:Fimbriae Proteins