title: The first determination of Trichuris sp. from roe deer by amplification and sequenation of the ITS1-5.8S-ITS2 segment of ribosomal DNA.
abstract: Trichuris nematodes were isolated from roe deer (Capreolus capreolus). At first, nematodes were determined using morphological and biometrical methods. Subsequently genomic DNA was isolated and the ITS1-5.8S-ITS2 segment from ribosomal DNA (RNA) was amplified and sequenced using PCR techniques. With u sing morphological and biometrical methods, female nematodes were identified as Trichuris globulosa, and the only male was identified as Trichuris ovis. The females were classified into four morphotypes. However, analysis of the internal transcribed spacers (ITS1-5.8S-ITS2) of specimens did not confirm this classification. Moreover, the female individuals morphologically determined as T. globulosa were molecularly identified as Trichuris discolor. In the case of the only male molecular analysis match the result of the molecular identification. Furthermore, a comparative phylogenetic study was carried out with the ITS1 and ITS2 sequences of the Trichuris species from various hosts. A comparison of biometric information from T. discolor individuals from this study was also conducted.
authors.affiliation: Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Kamycka 129, 165 21, Prague 6-Suchdol, Czech Republic.
authors.forename: O
authors.initials: O
authors.lastname: Salaba
authors.forename: K
authors.initials: K
authors.lastname: Rylková
authors.forename: J
authors.initials: J
authors.lastname: Vadlejch
authors.forename: M
authors.initials: M
authors.lastname: Petrtýl
authors.forename: S
authors.initials: S
authors.lastname: Scháňková
authors.forename: A
authors.initials: A
authors.lastname: Brožová
authors.forename: I
authors.initials: I
authors.lastname: Jankovská
authors.forename: L
authors.initials: L
authors.lastname: Jebavý
authors.forename: I
authors.initials: I
authors.lastname: Langrová
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D003670:Deer / Q000469:parasitology*; D005260:Female; D008297:Male; D008853:Microscopy; D008969:Molecular Sequence Data; D010802:Phylogeny; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014257:Trichuriasis / Q000469:parasitology / Q000662:veterinary*; D014258:Trichuris / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D018509:DNA, Helminth; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012340:RNA, Ribosomal, 5.8S