title: Microbulbifer mangrovi sp. nov., a polysaccharide-degrading bacterium isolated from an Indian mangrove.
abstract: A rod-shaped, Gram-negative, non-motile, aerobic and non-endospore forming bacterium, designated strain DD-13(T), was isolated from the mangrove ecosystem of Goa, India. Strain DD-13(T) degraded polysaccharides such as agar, alginate, chitin, cellulose, laminarin, pectin, pullulan, starch, carrageenan, xylan and β-glucan. The optimum pH and temperature for growth was 7 and 36 °C, respectively. The strain grew optimally in the presence of 3 % NaCl (w/v). The DNA G+C content was 61.4 mol%. The predominant fatty acid of strain DD-13(T) was iso-C15 : 0. Ubiquinone-8 was detected as the major respiratory lipoquinone. Phylogenetic studies based on 16S rRNA gene sequence analysis demonstrated that strain DD-13(T) formed a coherent cluster with species of the genus Microbulbifer. Strain DD-13(T) exhibited 16S rRNA gene sequence similarity levels of 98.9-97.1 % with Microbulbifer hydrolyticus IRE-31(T), Microbulbifer salipaludis JCM 11542(T), Microbulbifer agarilyticus JAMB A3(T), Microbulbifer celer KCTC 12973(T) and Microbulbifer elongatus DSM 6810(T). However, the level of DNA-DNA relatedness between strain DD-13(T) and the five type strains of these species of the genus Microbulbifer were in the range of 26-33 %. Additionally, strain DD-13(T) demonstrates several phenotypic differences from these type strains of species of the genus Microbulbifer. Thus strain DD-13(T) represents a novel species of the genus Microbulbifer, for which the name Microbulbifer mangrovi sp. nov. is proposed with the type strain DD-13(T) ( = KCTC 23483(T) = JCM 17729(T)).
authors.affiliation: Department of Biotechnology, Goa University, Taleigao Plateau, Goa, India.
authors.forename: Poonam
authors.initials: P
authors.lastname: Vashist
authors.affiliation: Extremobiosphere Research program, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15, Natsushima-cho, Yokosuka 237-0061, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Nogi
authors.affiliation: Department of Biotechnology, Goa University, Taleigao Plateau, Goa, India.
authors.forename: Sanjeev C
authors.initials: SC
authors.lastname: Ghadi
authors.affiliation: Molecular Biology Unit, National Centre for Cell Science, Pune University, Pune, India.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Verma
authors.affiliation: Molecular Biology Unit, National Centre for Cell Science, Pune University, Pune, India.
authors.forename: Yogesh S
authors.initials: YS
authors.lastname: Shouche
mesh_terms: D034241:Alteromonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8