title: [Determination method of muscone in rat intestinal perfusate by GC-MS/MS and its intestinal absorption kinetic characteristics in rats].
abstract: OBJECTIVE
  To establish the method for determining muscone in rat intestinal perfusate by GC-MS/MS and study its intestinal absorption kinetic characteristics in rats.
  
  
  METHODS
  The GC-MS/MS method was used to determine the content of muscone in rat intestinal circulation fluid. In situ intestinal circulation perfusion was adopted to study absorption kinetics of muscone in rats.
  
  
  RESULTS
  Muscone was proved to be well absorbed in each section of small intestine. Its absorption rate constants (Ka) and the absorption rate (A) in the rat intestine showed duodenum > jejunum (P < 0.05) , duodenum > ileum (P < 0.01). Its Ka, A and t1/2 in rat small intestine was 0.990 h(-1) , 43.58% and 0.705h, respectively.
  
  
  CONCLUSIONS
  Muscone was well absorbed in each intestinal section, with duodenum better than jejunum (Ka, T1/2, P < 0.05) significantly better than ileum (Ka, T1/2, P < 0.01; A, P < 0.05). There is no obvious statistical difference between jejunum and ileum.
authors.affiliation: College of Biological Industry, Chengdu University, Chengdu 610106, China. zouliang@cdu.edu.cn
authors.forename: Liang
authors.initials: L
authors.lastname: Zou
authors.forename: Junzhi
authors.initials: J
authors.lastname: Lin
authors.forename: Zhanguo
authors.initials: Z
authors.lastname: Wang
authors.forename: Lijia
authors.initials: L
authors.lastname: Xu
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
authors.forename: Jieying
authors.initials: J
authors.lastname: Luo
mesh_terms: D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D003516:Cycloparaffins / Q000032:analysis* / Q000493:pharmacokinetics*; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D003516:Cycloparaffins; C031021:muscone