title: Optogenetic control of transcription in zebrafish.
abstract: Light inducible protein-protein interactions are powerful tools to manipulate biological processes. Genetically encoded light-gated proteins for controlling precise cellular behavior are a new and promising technology, called optogenetics. Here we exploited the blue light-induced transcription system in yeast and zebrafish, based on the blue light dependent interaction between two plant proteins, blue light photoreceptor Cryptochrome 2 (CRY2) and the bHLH transcription factor CIB1 (CRY-interacting bHLH 1). We demonstrate the utility of this system by inducing rapid transcription suppression and activation in zebrafish.
authors.affiliation: National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research-Shanghai, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China. htliu@sibs.ac.cn
authors.forename: Hongtao
authors.initials: H
authors.lastname: Liu
authors.forename: Gustavo
authors.initials: G
authors.lastname: Gomez
authors.forename: Sophia
authors.initials: S
authors.lastname: Lin
authors.forename: Shuo
authors.initials: S
authors.lastname: Lin
authors.forename: Chentao
authors.initials: C
authors.lastname: Lin
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D056931:Cryptochromes / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000235:genetics / Q000528:radiation effects; D008027:Light; D062308:Optogenetics / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D014158:Transcription, Genetic / Q000235:genetics* / Q000528:radiation effects*; D015027:Zebrafish / Q000235:genetics* / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D056931:Cryptochromes