title: Discovery and verification of panels of T-lymphocyte proteins as biomarkers of Parkinson's disease.
abstract: The diagnosis of Parkinson's disease (PD) is currently based on the clinical evaluation of extrapyramidal signs with a considerable error rate. The identification of specific markers might allow PD diagnosis before the onset of classical motor symptoms. By two-dimensional electrophoresis we identified proteome alterations in T-lymphocytes of 17 control subjects and 15 PD patients. The observed changes were used to build predictive models that were verified by the leave-one-out cross-validation. We further built two functions able to stage the subjects. We chose to verify by Western blotting the identity of spots corresponding to β-fibrinogen and transaldolase, two recurrent proteins in six out of 20 spots. β-Fibrinogen levels are lowered in PD patients, whereas a heavy transaldolase set of isoforms was more abundant. Eventually, we identified a list of seven proteins showing different levels in early-onset with respect to late-onset PD patients.
authors.affiliation: Division of Biomedical Sciences, Department of Theoretical and Applied Sciences, University of Insubria , Busto Arsizio, Italy.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Alberio
authors.forename: Agnese C
authors.initials: AC
authors.lastname: Pippione
authors.forename: Maurizio
authors.initials: M
authors.lastname: Zibetti
authors.forename: Simone
authors.initials: S
authors.lastname: Olgiati
authors.forename: Daniela
authors.initials: D
authors.lastname: Cecconi
authors.forename: Cristoforo
authors.initials: C
authors.lastname: Comi
authors.forename: Leonardo
authors.initials: L
authors.lastname: Lopiano
authors.forename: Mauro
authors.initials: M
authors.lastname: Fasano
mesh_terms: D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D005340:Fibrinogen / Q000032:analysis; D006801:Humans; D010300:Parkinson Disease / Q000175:diagnosis*; D020033:Protein Isoforms / Q000032:analysis; D020543:Proteome / Q000032:analysis*; D013601:T-Lymphocytes / Q000737:chemistry*; D014153:Transaldolase / Q000032:analysis
chemical_list: D015415:Biomarkers; D020033:Protein Isoforms; D020543:Proteome; D005340:Fibrinogen; D014153:Transaldolase