title: Cardioprotective effects of cysteine alone or in combination with taurine in diabetes.
abstract: This study was undertaken to examine the effects of dietary supplementation of cysteine and taurine in rats with diabetes induced with streptozotocin (STZ, 65 mg/kg body weight). Experimental animals were treated orally (by gavage) with cysteine (200 mg/kg) and taurine (400 mg/kg), alone or in combination, daily for 8 weeks. In one group, rats were also pretreated 3 weeks before the induction of diabetes (prevention arm) whereas in the other, the treatment was started 3 days after the induction of diabetes (reversal arm). Diabetes increased heart weight/body weight (HW/BW) ratio, plasma glucose, triglyceride and cholesterol levels as well as depressed heart rate (HR), blood pressure, left ventricular systolic pressure (LVSP), rate of contraction (+dP/dt), rate of relaxation (-dP/dt), fractional shortening (FS) and cardiac output (CO). The left ventricular internal diameter in systole (LViDs) was increased whereas that in diastole (LViDd) was decreased. In the prevention arm, treatment of the diabetic animals with cysteine or taurine decreased HW/BW ratio and improved HR, FS, +dP/dt and -dP/dt, as well as normalized LViDs, without altering the increase in glucose level. Cysteine decreased plasma triglyceride and cholesterol levels and improved LVSP whereas CO was improved by taurine. In the reversal arm, cysteine alone or with taurine did not correct the changes in hemodynamic parameters, FS and plasma triglycerides. Diabetes-induced cardiac dysfunction and increases in plasma triglycerides can be prevented, but not reversed, by dietary cysteine alone or in combination with taurine.
authors.affiliation: Asper Clinical Research Institute, Winnipeg, Manitoba, Canada. ptappia@sbrc.ca
authors.forename: P S
authors.initials: PS
authors.lastname: Tappia
authors.forename: Y-J
authors.initials: YJ
authors.lastname: Xu
authors.forename: D
authors.initials: D
authors.lastname: Rodriguez-Leyva
authors.forename: N
authors.initials: N
authors.lastname: Aroutiounova
authors.forename: N S
authors.initials: NS
authors.lastname: Dhalla
mesh_terms: D000818:Animals; D002302:Cardiac Output / Q000187:drug effects; D002316:Cardiotonic Agents; D002784:Cholesterol / Q000097:blood; D003545:Cysteine / Q000627:therapeutic use*; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D058065:Diabetic Cardiomyopathies / Q000000981:diagnostic imaging / Q000517:prevention & control*; D004359:Drug Therapy, Combination; D006439:Hemodynamics / Q000187:drug effects / Q000502:physiology; D007328:Insulin / Q000097:blood; D008297:Male; D009929:Organ Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D013654:Taurine / Q000627:therapeutic use*; D014280:Triglycerides / Q000097:blood; D014463:Ultrasonography; D016277:Ventricular Function, Left / Q000187:drug effects; D016278:Ventricular Function, Right / Q000187:drug effects
chemical_list: D002316:Cardiotonic Agents; D007328:Insulin; D014280:Triglycerides; D013654:Taurine; D002784:Cholesterol; D003545:Cysteine