title: Lysine biosynthesis in bacteria: a metallodesuccinylase as a potential antimicrobial target.
abstract: In this review, we summarize the recent literature on dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase (DapE) enzymes, with an emphasis on structure-function studies that provide insight into the catalytic mechanism. Crystallographic data have also provided insight into residues that might be involved in substrate and hence inhibitor recognition and binding. These data have led to the design and synthesis of several new DapE inhibitors, which are described along with what is known about how inhibitors interact with the active site of DapE enzymes, including the efficacy of a moderately strong DapE inhibitor.
authors.affiliation: Department of Chemistry and Biochemistry, Loyola University-Chicago, 1068 W. Sheridan Rd., Chicago, IL, 60626, USA. danuta.gilner@polsl.pl.|Department of Chemistry, Silesian University of Technology, ul. Krzywoustego 4, 44-100, Gliwice, Poland. danuta.gilner@polsl.pl.
authors.forename: Danuta M
authors.initials: DM
authors.lastname: Gillner
authors.affiliation: Department of Chemistry and Biochemistry, Loyola University-Chicago, 1068 W. Sheridan Rd., Chicago, IL, 60626, USA.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Becker
authors.affiliation: Department of Chemistry and Biochemistry, Loyola University-Chicago, 1068 W. Sheridan Rd., Chicago, IL, 60626, USA. rholz1@luc.edu.
authors.forename: Richard C
authors.initials: RC
authors.lastname: Holz
mesh_terms: D000581:Amidohydrolases / Q000037:antagonists & inhibitors* / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000201:enzymology*; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors* / Q000737:chemistry; D020134:Catalytic Domain; D024881:Drug Resistance, Bacterial; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008239:Lysine / Q000096:biosynthesis*; D008958:Models, Molecular; D011485:Protein Binding
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D000581:Amidohydrolases; D008239:Lysine