title: Myceligenerans salitolerans sp. nov., a halotolerant actinomycete isolated from a salt lake in Xinjiang, China.
abstract: A halotolerant actinomycete strain, designated XHU 5031(T), was isolated from a salt lake in Xinjiang Province, northwest China. Its taxonomic status was determined using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the organism was most closely related to Myceligenerans xiligouense DSM 15700(T) (98.4 %), Myceligenerans halotolerans XJEEM 11063(T) (98.0 %) and Myceligenerans crystallogenes DSM 17134(T) (97.5 %). However, it had relatively low values for DNA-DNA relatedness with the above strains (46.2, 39.4 and 36.5 %, respectively). The peptidoglycan type was A4α. This organism contained glucose, mannose and galactose as the major whole cell sugars. The predominant menaquinone was MK-9(H(4)). The major fatty acids were iso-C(15:0,) anteiso-C(15:0) and iso-C(16:0). The polar lipids detected were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI), one unknown phospholipid (PL) and two unknown glycolipids (GL). The G+C content of genomic DNA was 71.2 mol %. Phenotypic data clearly distinguished the isolate from its closest relatives. The combined genotypic and phenotypic data indicated that the isolate XHU 5031(T) represented a novel species of the genus Myceligenerans. The proposed name for this organism is Myceligenerans                 salitolerans sp. nov., with type strain XHU 5031(T) (=KCTC 29128(T) = CCTCC AB 2012908(T)).
authors.affiliation: School of Bioengineering, Xihua University, Chengdu, 610039, People's Republic of China. uantongweily@163.com
authors.forename: Tong-Wei
authors.initials: TW
authors.lastname: Guan
authors.forename: Nan
authors.initials: N
authors.lastname: Wu
authors.forename: Shu-Kun
authors.initials: SK
authors.lastname: Tang
authors.forename: Zhen-Ming
authors.initials: ZM
authors.lastname: Che
authors.forename: Xiang-Gui
authors.initials: XG
authors.lastname: Chen
mesh_terms: D039903:Actinobacteria / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism*; D001483:Base Sequence; D002681:China; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014871:Water Microbiology
chemical_list: D008563:Membrane Lipids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S