title: [Expression of Fbxw7 and its correlation with cell proliferation in human hepatocellular carcinoma].
abstract: OBJECTIVE
  To observe the expression of Fbxw7 and explore its correlation with cell proliferation of hepatocellular carcinoma (HCC).
  
  
  METHODS
  The expression of Fbxw7 in paired tumor and tumor-adjacent normal tissues from 40 patients with HCC were assessed using RT-PCR and immunohistochemistry. The expression level of Fbxw7 mRNA in normal liver cell line (LO2) and HCC cell lines (Hep3B and SMMC-7721) were detected by real-time RT-PCR. Colony formation assay and tumor xenograft assay were performed in different cell lines with different Fbxw7 expression respectively to investigate the ability of cell proliferation in vitro and in vivo.
  
  
  RESULTS
  The expression of Fbxw7 at both mRNA and protein levels in HCC tissues were significantly down-regulated compared to tumor-adjacent normal tissues (P<0.05). Down-regulation of Fbxw7 was found apparently associated with high Edmonson-Steiner classification and advanced TNM stage (P<0.05). The expression of Fbxw7 mRNA in LO2 was significantly higher than that in Hep3B or SMMC-7721 (P<0.05). Cell lines with less Fbxw7 expression had not only more colonies in colony formation assay (P<0.05),  but also bigger xenografts in vitro (P<0.05).
  
  
  CONCLUSIONS
  The low expression of Fbxw7 correlates with the poor clinicopathological characteristics and cell proliferation of HCC.
authors.affiliation: Department of Hepatobiliary Surgery,  First Affiliated Hospital,  Medical College,  Xi'an Jiaotong University,  Xi'an  710061,  China.
authors.forename: Zhen-yu
authors.initials: ZY
authors.lastname: Zhou
authors.forename: Kang-sheng
authors.initials: KS
authors.lastname: Tu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Zheng
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.forename: Ying-min
authors.initials: YM
authors.lastname: Yao
authors.forename: Qing-guang
authors.initials: QG
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology*; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D044783:F-Box Proteins / Q000235:genetics* / Q000378:metabolism; D000073979:F-Box-WD Repeat-Containing Protein 7; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D044767:Ubiquitin-Protein Ligases / Q000235:genetics* / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D044783:F-Box Proteins; D000073979:F-Box-WD Repeat-Containing Protein 7; C513273:FBXW7 protein, human; D044767:Ubiquitin-Protein Ligases