title: Quantitative optical trapping on single organelles in cell extract.
abstract: We have developed an optical trapping method to precisely measure the force generated by motor proteins on single organelles of unknown size in cell extract. This approach, termed VMatch, permits the functional interrogation of native motor complexes. We apply VMatch to measure the force, number and activity of kinesin-1 on motile lipid droplets isolated from the liver of normally fed and food-deprived rats.
authors.affiliation: Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, India.
authors.forename: Pradeep
authors.initials: P
authors.lastname: Barak
authors.forename: Ashim
authors.initials: A
authors.lastname: Rai
authors.forename: Priyanka
authors.initials: P
authors.lastname: Rai
authors.forename: Roop
authors.initials: R
authors.lastname: Mallik
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005215:Fasting; D016547:Kinesins / Q000378:metabolism*; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D008099:Liver / Q000166:cytology / Q000378:metabolism; D052898:Optical Tweezers; D015388:Organelles / Q000502:physiology*; D051381:Rats
chemical_list: D008055:Lipids; D016547:Kinesins