title: [A new method of identifying the peripheral blood and the menstrual blood].
abstract: OBJECTIVE
  To explore the tissue-specific gene expressions of the peripheral blood and the menstrual blood, and to search some specific factors to establish an effective method for identifying the peripheral blood and the menstrual blood.
  
  
  METHODS
  The specific products of the peripheral blood and the menstrual blood were detected by RT-PCR and separated by electrophoretic technology.
  
  
  RESULTS
  Beta-spectrin (SPTB) as one specific marker of peripheral blood and 18S rRNA as a kind of the housekeeping gene were expressed in both the peripheral blood and the menstrual blood. However, matrix metalloproteinase 7 (MMP7) as one specific marker of menstrual blood and human beta defensin 1 (HBD1) as one specific marker of vaginal discharge were only found in the menstrual blood.
  
  
  CONCLUSIONS
  There are differences of specific gene expressions between the peripheral blood and the menstrual blood. They could be accurately distinguished from each other by using the combination of fluorescence technology and RT-PCR to detect the specific identification of mRNA.
authors.affiliation: School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China. wangyingxi4722@163.com
authors.forename: Ying-xi
authors.initials: YX
authors.lastname: Wang
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Zhu
authors.forename: Zhang-ping
authors.initials: ZP
authors.lastname: Jiao
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Lu
authors.forename: Wei-ni
authors.initials: WN
authors.lastname: Wang
authors.forename: Ya-cheng
authors.initials: YC
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Tang
mesh_terms: D015415:Biomarkers; D001769:Blood / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D020783:Matrix Metalloproteinase 7 / Q000235:genetics; D008598:Menstruation / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023083:beta-Defensins
chemical_list: D015415:Biomarkers; C406407:DEFB1 protein, human; D012333:RNA, Messenger; D023083:beta-Defensins; D020783:Matrix Metalloproteinase 7