title: [Detection and correlation of lipopolysaccharide, vitamin D receptor and matrix metalloproteinase-9 in the middle ear cholesteatoma].
abstract: OBJECTIVE
  To investigate the expression and concentration of lipopolysaccharide (LPS) and matrix metalloproteinase-9 (MMP-9) in middle ear cholesteatoma and discuss their relations.
  
  
  METHODS
  Twenty-nine cases of middle ear cholesteatoma tissue, 18 cases of external auditory canal tissue were detected by limulus amebocyte lysate assay (LAL-assay), and expression of MMP-9 protein in formalin-fixed, paraffin-embedded tissues was detected by immunohistochemical method.
  
  
  RESULTS
  The concentrations of LPS in cholesteatoma were higher than that in external auditory canal tissues. In group of cholesteatoma: M = 0.739 0, IQR = 0.6203, and in group of external auditory canal tissues: M = -0.2538, IQR = 1.1692 (P < 0.01). In cholesteatoma groups, in extensive type: M = 0.8403, IQR = 0.5254; in localized type: M = 0.4048, IQR = 0.6139, the concentrations of LPS were higher in extensive cholesteatoma in comparison with localized cholesteatoma (P < 00.05). In cholesteatoma epithelium samples, MMP-9 were 79.3%. Compared with external auditory canal epithelium, the expression of MMP-9 was higher in middle ear cholesteatoma epithelium (P < 0.05). There was no significant difference in the expression of MMP-9 between two types of cholesteatoma epithelium (P > 0.05). LPS, MMP-9 weren't significantly correlated by Spearman test.
  
  
  CONCLUSIONS
  LPS was responsible for middle ear cholesteatoma and its related bone erosion. MMP-9 was related to the development of middle ear cholesteatoma. There's no correlation between LPS and MMP-9.
authors.affiliation: Department of Otolaryngology Head and Neck Surgery,No 303 Hospital of PLA, Nanning, 530021, China.
authors.forename: Yongling
authors.initials: Y
authors.lastname: Li
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Xu
authors.forename: Longcheng
authors.initials: L
authors.lastname: Zhang
authors.forename: Chaokun
authors.initials: C
authors.lastname: Quan
authors.forename: Xinran
authors.initials: X
authors.lastname: Lin
mesh_terms: D000328:Adult; D018424:Cholesteatoma, Middle Ear / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000378:metabolism*; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D018167:Receptors, Calcitriol / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D018167:Receptors, Calcitriol; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9