title: [Inhibitory effect of arsenic trioxide on invasion in human hepatocellular carcinoma SMMC-7721 cells and its mechanism].
abstract: OBJECTIVE
  To investigate the effects of arsenic trioxide (As(2);O(3);) on human hepatocellular carcinoma SMMC-7721 cells' migration and invasion and the underlying molecular mechanism.
  
  
  METHODS
  The effects of As(2);O(3); on cell migration and invasion were examined by wound-healing and Transwell assays;  The expressions of CD147 and MMP-2 at both mRNA and protein levels in SMMC-7721 cells treated with As(2);O(3); were determined by real-time PCR and Western blotting,  respectively.
  
  
  RESULTS
  The wound-healing assay showed that the scuffing distance of SMMC-7721 cells exposed to 2.0 μmol/L As(2);O(3); for 12 and 24 h was significantly wider as compared with the ones unexposed to As(2);O(3);,  indicating As(2);O(3); could inhibit the migration in SMMC-7721 cells (P<0.05). The Transwel1 assay showed that the number of SMMC-7721 cells migrating through the Matrigel treated by 2.0 μmol/L As(2);O(3); for 12 and 24 h obviously decreased as compared with non-treated ones,  indicating that As(2);O(3); could inhibit the invasion of SMMC-7721 cells (P<0.05). Real-time PCR showed that the expressions of CD147 and MMP-2 mRNA were down-regulated in SMMC-7721 cells treated by 2.0 μmol/L As(2);O(3); for 6,  12 and 24 h,  respectively (P<0.05). Western blotting demonstrated that the expressions of CD147 and MMP-2 proteins decreased in SMMC-7721 cells exposed to 2.0 μmol/L As(2);O(3); for 12,  24 and 48 h,  respectively (P<0.05).
  
  
  CONCLUSIONS
  As(2);O(3); could inhibit the migration and invasion in human hepatocellular carcinoma SMMC-7721 cells,  which may be related to the down-regulation of CD147 and MMP-2.
authors.affiliation: Department of Medical Technology, Xi'an Medical College, Xi'an 710021, China. lihaiyah319@126.com
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Li
authors.forename: Li-min
authors.initials: LM
authors.lastname: Cao
mesh_terms: D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D051926:Basigin / Q000235:genetics / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000187:drug effects*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D009361:Neoplasm Invasiveness; D010087:Oxides / Q000494:pharmacology*
chemical_list: D001152:Arsenicals; D010087:Oxides; D051926:Basigin; D020778:Matrix Metalloproteinase 2; D000077237:Arsenic Trioxide