title: Gene-for-gene tolerance to bacterial wilt in Arabidopsis.
abstract: Bacterial wilt caused by Ralstonia solanacearum is a disease of widespread economic importance that affects numerous plant species, including Arabidopsis thaliana. We describe a pathosystem between A. thaliana and biovar 3 phylotype I strain BCCF402 of R. solanacearum isolated from Eucalyptus trees. A. thaliana accession Be-0 was susceptible and accession Kil-0 was tolerant. Kil-0 exhibited no wilting symptoms and no significant reduction in fitness (biomass, seed yield, and germination efficiency) after inoculation with R. solanacearum BCCF402, despite high bacterial numbers in planta. This was in contrast to the well-characterized resistance response in the accession Nd-1, which limits bacterial multiplication at early stages of infection and does not wilt. R. solanacearum BCCF402 was highly virulent because the susceptible accession Be-0 was completely wilted after inoculation. Genetic analyses, allelism studies with Nd-1, and RRS1 cleaved amplified polymorphic sequence marker analysis showed that the tolerance phenotype in Kil-0 was dependent upon the resistance gene RRS1. Knockout and complementation studies of the R. solanacearum BCCF402 effector PopP2 confirmed that the tolerance response in Kil-0 was dependent upon the RRS1-PopP2 interaction. Our data indicate that the gene-for-gene interaction between RRS1 and PopP2 can contribute to tolerance, as well as resistance, which makes it a useful model system for evolutionary studies of the arms race between plants and bacterial pathogens. In addition, the results alert biotechnologists to the risk that deployment of RRS1 in transgenic crops may result in persistence of the pathogen in the field.
authors.affiliation: Department of Plant Science, Forestry and Agricultural Research Institute, South Africa.
authors.forename: Liesl
authors.initials: L
authors.lastname: Van der Linden
authors.forename: Jane
authors.initials: J
authors.lastname: Bredenkamp
authors.forename: Sanushka
authors.initials: S
authors.lastname: Naidoo
authors.forename: Joanne
authors.initials: J
authors.lastname: Fouché-Weich
authors.forename: Katherine J
authors.initials: KJ
authors.lastname: Denby
authors.forename: Stephane
authors.initials: S
authors.lastname: Genin
authors.forename: Yves
authors.initials: Y
authors.lastname: Marco
authors.forename: Dave K
authors.initials: DK
authors.lastname: Berger
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology; D011485:Protein Binding / Q000235:genetics / Q000502:physiology; D043368:Ralstonia solanacearum / Q000378:metabolism* / Q000472:pathogenicity*
chemical_list: D029681:Arabidopsis Proteins; D001426:Bacterial Proteins