title: Assessment of cytokine release after in vitro stimulation of whole blood with legionella pneumophila in immunocompromised patients.
abstract: This study we examined ex vivo potential of the immune response after stimulation of whole blood with L. pneumophila SG 1, SG 2-14 and L. pneumophila standard strain ATCC 33152 in immunocompromised patients, such as: hemodialysis patients and patients after renal transplantation. The levels of TNF-α and IFN-γ in supernatants were measured with the use of commercial ELISA kits. The synthesis of TNF-α and IFN-γ after stimulation with L. pneumophila were analyzed in two aspects: differentiated stimulatory activity in relation to SG 1, SG 2-14 and ATCC 33152 L. pneumophila and differentiated response of the hemodialysis patients and patients after renal transplantation in relation to the control group. The positive and negative results of anti-L. pneumophila antibodies of two groups of our patients were found for the analysis of the stimulatory activity of L.pneumophila as a primary or secondary response. In patients with immunosuppression the response in the secretion of cytokines (TNF-α and IFN-γ) was reduced after stimulation of L. pneumophila SG 1 but in varying degrees after stimulation of L. pneumophila SG 2-14, which indicates that the risk of the infection is varied.
authors.affiliation: Department of Medical Microbiology, Medical University of Lublin, Poland. agnieszka.sikora24@wp.pl
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Sikora
authors.forename: Maria
authors.initials: M
authors.lastname: Kozioł-Montewka
authors.forename: Andrzej
authors.initials: A
authors.lastname: Książek
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Wójtowicz
authors.forename: Jolanta
authors.initials: J
authors.lastname: Paluch-Oleś
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Magryś
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Grzebalska
authors.forename: Anna
authors.initials: A
authors.lastname: Bednarek-Skublewska
authors.forename: Anna
authors.initials: A
authors.lastname: Steć
authors.forename: Sławomir
authors.initials: S
authors.lastname: Rudzki
authors.forename: Jacek
authors.initials: J
authors.lastname: Furmaga
authors.forename: Renata
authors.initials: R
authors.lastname: Matuszewska
authors.forename: Bożena
authors.initials: B
authors.lastname: Krogulska
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001773:Blood Cells / Q000276:immunology*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007114:Immunization / Q000379:methods*; D016867:Immunocompromised Host / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism*; D016030:Kidney Transplantation; D016952:Legionella pneumophila / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D055815:Young Adult
chemical_list: D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma