title: Possible ATP release through lysosomal exocytosis from primary sensory neurons.
abstract: The adenosine triphosphate (ATP) plays important roles under physiological and pathological conditions such as traumatic brain injury, neuroinflammation and neuropathic pain. In the present study, we set out to study the role of lysosomal vesicles on ATP release from the dorsal root ganglion neurons. We found that the lysosomal vesicles, which contain the quinacrine-positive fluorescence and express the vesicular nucleotide transporter (VNUT), were localized within the soma and growth cone of the cultured dorsal root ganglion neurons. In addition, the number of the quinacrine staining was decreased by application of lysosomal exocytosis activators, and this decrease was suppressed by the metformin and vacuolin-1, which suppressed lysosomal exocytosis. Thus, these findings suggest that ATP release via the lysosomal exocytosis may be one of the pathways for ATP release in response to stimulation.
authors.affiliation: Department of Anatomy, College of Medicine, Kyung Hee University, Heoki-Dong 1, Dongdaemun-Gu, Seoul 130-701, Republic of Korea.
authors.forename: Junyang
authors.initials: J
authors.lastname: Jung
authors.forename: Youn Ho
authors.initials: YH
authors.lastname: Shin
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Konishi
authors.forename: Seo Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kiyama
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D002478:Cells, Cultured; D005089:Exocytosis; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism*; D008297:Male; D009475:Neurons, Afferent / Q000378:metabolism* / Q000648:ultrastructure; D033704:Nucleotide Transport Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D033704:Nucleotide Transport Proteins; D000255:Adenosine Triphosphate