title: Time-resolved singlet oxygen luminescence detection under photodynamic therapy relevant conditions: comparison of ex vivo application of two photosensitizer formulations.
abstract: Singlet oxygen plays a crucial role in photo-dermatology and photodynamic therapy (PDT) of cancer. Its direct observation by measuring the phosphorescence at 1270 nm, however, is still challenging due to the very low emission probability. It is especially challenging for the time-resolved detection of singlet oxygen kinetics in vivo which is of special interest for biomedical applications. Photosensitized generation of singlet oxygen, in pig ear skin as model for human skin, is investigated here. Two photosensitizers (PS) were topically applied to the pig ear skin and examined in a comparative study, which include the amphiphilic pheophorbide-a and the highly hydrophobic perfluoroalkylated zinc phthalocyanine (F64PcZn). Fluorescence microscopy indicates the exclusive accumulation of pheophorbide-a in the stratum corneum, while F64PcZn can also accumulate in deeper layers of the epidermis of the pig ear skin. The kinetics obtained with phosphorescence measurements show the singlet oxygen interaction with the PS microenvironment. Different generation sites of singlet oxygen correlate with the luminescence kinetics. The results show that singlet oxygen luminescence detection can be used as a diagnostic tool, not only for research, but also during treatment. The detection methodology is suitable for the monitoring of chemical quenchers’ oxidation as well as saturation at singlet oxygen concentration levels relevant to PDT treatment protocols.
authors.affiliation: Humboldt-Universitätzu Berlin, Institute of Physics, Newtonstr. 15, 12489 Berlin, Germany.
authors.forename: Jan C
authors.initials: JC
authors.lastname: Schlothauer
authors.forename: Steffen
authors.initials: S
authors.lastname: Hackbarth
authors.forename: Lutz
authors.initials: L
authors.lastname: Jäger
authors.forename: Kai
authors.initials: K
authors.lastname: Drobniewski
authors.forename: Hemantbhai
authors.initials: H
authors.lastname: Patel
authors.forename: Sergiu M
authors.initials: SM
authors.lastname: Gorun
authors.forename: Beate
authors.initials: B
authors.lastname: Röder
mesh_terms: D000818:Animals; D002734:Chlorophyll / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D054833:Isoindoles; D008163:Luminescent Measurements; D036641:Microscopy, Fluorescence, Multiphoton; D010778:Photochemotherapy; D010782:Photolysis; D017319:Photosensitizing Agents / Q000494:pharmacology*; D026082:Singlet Oxygen / Q000032:analysis*; D012867:Skin / Q000737:chemistry / Q000187:drug effects; D013552:Swine
chemical_list: D007211:Indoles; D054833:Isoindoles; D017319:Photosensitizing Agents; D002734:Chlorophyll; D026082:Singlet Oxygen; C032623:pheophorbide a; C013647:phthalocyanine