title: Expression of RON in colorectal cancer and its relationships with tumor cell behavior and prognosis.
abstract: OBJECTIVE
  The aims of the current study were to evaluate whether recepteur d'origine nantais (RON) affects tumor cell behavior and oncogenic signaling pathways in colorectal cancer, and to examine the relationship of its expression with various clinicopathological parameters and patient survival.
  
  
  METHODS
  Immunohistochemistry, Western blot and RT-PCR were used to detect the expression of the RON gene in human colorectal cancer tissue. To study the biological role of RON in tumor cell behavior and cellular signaling pathways, we used small interfering RNA (siRNA) to knock down RON gene expression in human colorectal cancer cell lines.
  
  
  RESULTS
  Knockdown of RON inhibited the induction of the invasive growth phenotype and the activation of oncogenic signaling pathways including Akt, MAPK and β-catenin. RON overexpression was associated with tumor size, lymphovascular invasion, depth of invasion, lymph node metastasis, distant metastasis, tumor stage and poor survival.
  
  
  CONCLUSIONS
  These results suggest that RON overexpression may help in predicting poor clinical outcomes in colorectal cancer.
authors.forename: Young-Lan
authors.initials: YL
authors.lastname: Park
authors.forename: Gi-Hoon
authors.initials: GH
authors.lastname: Lee
authors.forename: Kyu-Yeol
authors.initials: KY
authors.lastname: Kim
authors.forename: Eun
authors.initials: E
authors.lastname: Myung
authors.forename: Jong-Sun
authors.initials: JS
authors.lastname: Kim
authors.forename: Dae-Seong
authors.initials: DS
authors.lastname: Myung
authors.forename: Kang-Jin
authors.initials: KJ
authors.lastname: Park
authors.forename: Sung-Bum
authors.initials: SB
authors.lastname: Cho
authors.forename: Wan-Sik
authors.initials: WS
authors.lastname: Lee
authors.forename: Young-Do
authors.initials: YD
authors.lastname: Jung
authors.forename: Hyun-Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Young-Eun
authors.initials: YE
authors.lastname: Joo
mesh_terms: D000368:Aged; D003106:Colon / Q000737:chemistry / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008198:Lymph Nodes / Q000378:metabolism* / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009361:Neoplasm Invasiveness; D011237:Predictive Value of Tests; D011379:Prognosis; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism*; D015398:Signal Transduction; D015854:Up-Regulation; D051176:beta Catenin / Q000378:metabolism
chemical_list: D051176:beta Catenin; C080835:RON protein; D020794:Receptor Protein-Tyrosine Kinases; D051057:Proto-Oncogene Proteins c-akt; D020928:Mitogen-Activated Protein Kinases