title: PKCι counteracts oxidative stress by regulating Hsc70 in an esophageal cancer cell line.
abstract: Using a glutathione S-transferase pull-down liquid chromatography-coupled tandem mass spectrometry approach and immunoprecipitation/immunoblot analysis, we found that heat shock cognate protein 70 (Hsc70) was involved in the complex formed by atypical protein kinase Cι (PKCι) and LC3 in the esophageal cancer cell line KYSE30. Further study indicated that Hsc70 was targeted by autophagic degradation, and knockdown of PKCι down-regulated Hsc70 by promoting autophagy. PKCι knockdown sensitized cells to oxidative stress-induced apoptosis, whereas forced PKCι expression counteracted the oxidative stress-induced apoptosis via Hsc70.
authors.affiliation: State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, 17 Panjiayuan Nanli, Chaoyang District, Beijing, 100021, China.
authors.forename: Bo-Shi
authors.initials: BS
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Hai
authors.initials: H
authors.lastname: Yang
authors.forename: Yi-Zhen
authors.initials: YZ
authors.lastname: Liu
authors.forename: Jia-Jie
authors.initials: JJ
authors.lastname: Hao
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhi-Zhou
authors.initials: ZZ
authors.lastname: Shi
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Jia
authors.forename: Qi-Min
authors.initials: QM
authors.lastname: Zhan
authors.forename: Ming-Rong
authors.initials: MR
authors.lastname: Wang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D017209:Apoptosis; D001343:Autophagy; D045744:Cell Line, Tumor; D019610:Cytoprotection; D004938:Esophageal Neoplasms / Q000201:enzymology* / Q000473:pathology; D050883:HSC70 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D007527:Isoenzymes / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D018384:Oxidative Stress; D011485:Protein Binding; D011493:Protein Kinase C / Q000378:metabolism*; D000071456:Sequestosome-1 Protein; D000099293:Protein Kinase C-lambda
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D050883:HSC70 Heat-Shock Proteins; D007527:Isoenzymes; D008869:Microtubule-Associated Proteins; D011493:Protein Kinase C; D000071456:Sequestosome-1 Protein; D000099293:Protein Kinase C-lambda; C494371:HSPA8 protein, human; C477012:MAP1LC3A protein, human; C099718:SQSTM1 protein, human