title: Expression profiles of peroxiredoxins in liver stage of the rodent malaria parasite Plasmodium berghei.
abstract: mRNA and protein expression profiles for three peroxiredoxins (TPx-1, TPx-2 and 1-Cys Prx) of liver stage Plasmodium berghei were examined through quantitative reverse transcription-PCR (RT-PCR) and indirect immunofluorescence microscopy assay (IFA). RT-PCR experiments revealed that mRNA expression for the TPx-1 was detected shortly after the sporozoite infection and kept expressed until the schizont stage. In contrast, the mRNA expression for 1-Cys Prx had begun increasing when the parasite developed into the schizont stage. Using the IFA, TPx-1 and 1-Cys Prx were detected in the cytosol. This finding suggested the developmental stage-specific expression of the cytosolic enzymes in the liver stage parasite. On the other hand, the mRNA expression for TPx-2 had begun increasing at the trophozoite stage and peaked at the schizont stage. In the IFA, TPx-2 was found localized in the mitochondria. The increase of TPx-2 might be explained by the exponential development of the parasite during the schizont stage requiring ATP production which may induce reactive oxygen species (ROS) in the mitochondria.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, 2-13 Inada-cho, Obihiro, Hokkaido, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Usui
authors.forename: Hirono
authors.initials: H
authors.lastname: Masuda-Suganuma
authors.forename: Shinya
authors.initials: S
authors.lastname: Fukumoto
authors.forename: Jose Ma M
authors.initials: JM
authors.lastname: Angeles
authors.forename: Noboru
authors.initials: N
authors.lastname: Inoue
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Kawazu
mesh_terms: D000818:Animals; D003600:Cytosol / Q000201:enzymology; D019084:Fluorescent Antibody Technique, Indirect; D015971:Gene Expression Regulation, Enzymologic; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver / Q000469:parasitology; D008288:Malaria / Q000201:enzymology / Q000469:parasitology*; D052940:Merozoites; D008928:Mitochondria / Q000201:enzymology; D054464:Peroxiredoxins / Q000235:genetics* / Q000378:metabolism; D010962:Plasmodium berghei / Q000201:enzymology* / Q000235:genetics; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D016053:RNA, Protozoan / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D034101:Sporozoites
chemical_list: D015800:Protozoan Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan; D017382:Reactive Oxygen Species; D054464:Peroxiredoxins