title: [PB1-F2 protein of influenza A virus interacts with human MOAP-1 protein].
abstract: OBJECTIVE
  To identify the interaction between influenza A virus PB1-F2 and human modulator of apoptosis 1 (MOAP-1).
  
  
  METHODS
  The recombinant plasmid pACT2-MOAP-1 was constructed and co-transformed into yeast AH109 with pGBKT7-PB1-F2. The growth of the co-transformants on quadruple dropout medium and beta-galactosidase activity of the reporter gene were tested. We further confirmed the interaction of cellular protein MOAP-1 and PB1-F2 by glutathione S-transferase (GST) pull-down and co-immunoprecipitation (Co-IP) assays. In addition, we investigated the effect of PB1-F2 on MOAP-1 protein level by Western blot.
  
  
  RESULTS
  The results of yeast two-hybrid assay showed that MOAP-1 specifically interacted with PB1-F2 in yeast cells. Furthermore, the binding of MOAP-1 with PB1-F2 was demonstrated by glutathione S-transferase pull-down and Co-IP assays. PB1-F2 could upregulate exogenous MOAP-1 protein level.
  
  
  CONCLUSIONS
  These results suggested that influenza virus PB1-F2 interacted with MOAP-1 and it might be involved in the regulation of cell growth and apoptosis via association with MOAP-1.
authors.affiliation: Key Laboratory of Zoonosis Ministry of Education, institute of zoonosis, Jilin University, Changchun 130062, China. cuiyuming25@163.com
authors.forename: Yuming
authors.initials: Y
authors.lastname: Cui
authors.forename: Peili
authors.initials: P
authors.lastname: Hou
authors.forename: Maolin
authors.initials: M
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Duan
authors.forename: Zhenhong
authors.initials: Z
authors.lastname: Guan
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000378:metabolism*; D007251:Influenza, Human / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; C428449:MOAP1 protein, human; C442816:PB1-F2 protein, Influenza A virus; D014764:Viral Proteins