title: Expression profiles of phosphatidylinositol phosphate kinase genes  during normal human in vitro erythropoiesis.
abstract: Phosphatidylinositol phosphate kinases (PIPKs) are  enzymes that participate in diverse intracellular signaling pathways. They are  classified into 3 functionally distinct subfamilies - PIPKI (α, β, γ), PIPKII  (α, β, γ), and PIPKIII - located in various subcellular compartments. Recently,  the PIPKIIα and β-globin genes were found to be overexpressed in reticulocytes  from 2 siblings with hemoglobin H disease, suggesting a possible relationship  between PIPKIIa and the production of globins. The main aim of this study was  to determine the expression profiles of PIPK genes in healthy individuals  during in vitro erythropoiesis using quantitative real-time polymerase  chain reaction and to compare these profiles with profiles of globin genes. Our  results showed that expression of all PIPKs increases as the cells  differentiate, coinciding with the expression profiles of globins. Analysis of  the effects of globins on PIPK genes revealed that they varied significantly between  the globins, the most noticeable being the effect of α-globin on PIPKIIα (P  < 0.0001) and γ-globin on PIPKIIγ (P < 0.0001). The relationship between  the expression of PIPKs and globin genes was statistically significant,  particularly between PIPKIIα and α-globin (P = 0.0002) and PIPKIIγ and β-globin  (P < 0.0001). Linear correlation analysis revealed a strong relationship between  PIPKIIα and α-globin genes. This study is the first to establish the expression  profiles of PIPK genes during in vitro erythropoiesis in healthy  individuals and suggests a parallel between the expression of PIPK and globin  genes, reinforcing the hypothesis that they may be related.
authors.affiliation: Departamento de Patologia Clínica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Campinas, SP, Brasil.
authors.forename: T R
authors.initials: TR
authors.lastname: Zaccariotto
authors.forename: C
authors.initials: C
authors.lastname: Lanaro
authors.forename: D M
authors.initials: DM
authors.lastname: Albuquerque
authors.forename: M N N
authors.initials: MN
authors.lastname: Santos
authors.forename: M A C
authors.initials: MA
authors.lastname: Bezerra
authors.forename: F G P
authors.initials: FG
authors.lastname: Cunha
authors.forename: I
authors.initials: I
authors.lastname: Lorand-Metze
authors.forename: A S
authors.initials: AS
authors.lastname: Araujo
authors.forename: F F
authors.initials: FF
authors.lastname: Costa
authors.forename: M F
authors.initials: MF
authors.lastname: Sonati
mesh_terms: D000328:Adult; D019540:Area Under Curve; D004920:Erythropoiesis / Q000235:genetics*; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D005914:Globins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics* / Q000378:metabolism
chemical_list: D005914:Globins; D017853:Phosphotransferases (Alcohol Group Acceptor)