title: [Inhibition of virus infection in coxsackievirus-induced myocarditis model by RNA interference].
abstract: To evaluate the effects of lentivirus-delivered short hairpin RNA (shRNA) on CVB3 infection in an animal model by RNA interference technique, we constructed a recombinant lentivirus expressing shRNA-3753 against the viral genome region 3753-3771, then transduced Lenti-sh3753 into mice infected with CVB3. We evaluated the antiviral ability of lenti-sh3753 by cytopathic effect (CPE), viral plaque assay and histological analysis of mice hearts. The results showed that Lenti-sh3753 exhibited a significant protective effect on cell viability and reduction of viral titers in supernatant of cell culture by specific inhibition on viral replication. Lenti-sh3753 also prolonged the mice survival and limited the viral production in mice hearts. These data proposed that Lenti-sh3753 can effectively inhibit CVB3 infection in a coxsackievirus-induced myocarditis model, suggesting its potential role in prevention and therapy of viral diseases.
authors.affiliation: Capital Institute of Paediatrics, Beijing 100020, China. helen919@163.com
authors.forename: Hai-Lan
authors.initials: HL
authors.lastname: Yao
authors.forename: Feng
authors.initials: F
authors.lastname: He
authors.forename: Zong-Hui
authors.initials: ZH
authors.lastname: Xiao
authors.forename: Zhe-Wei
authors.initials: ZW
authors.lastname: Liu
mesh_terms: D000818:Animals; D003384:Coxsackievirus Infections / Q000188:drug therapy / Q000821:virology*; D015536:Down-Regulation; D029822:Enterovirus B, Human / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000188:drug therapy / Q000821:virology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics* / Q000627:therapeutic use; D012367:RNA, Viral / Q000235:genetics; D014779:Virus Replication
chemical_list: D034741:RNA, Small Interfering; D012367:RNA, Viral