title: Neutraceutical approaches to control diabetes: A natural requisite approach.
abstract: OBJECTIVE
  The aim of this study is to screen the polyherbal preparation for antidiabetic activity in rats.
  
  
  METHODS
  The blood glucose lowering activity of the polyherbal preparation-I (1:1:1 of wheat germ oil, Coraidrum sativum, and Aloe vera) was studied in normal rats after oral administration at doses of 1.0 ml/kg and 2.0 ml/kg and polyherbal preparation-I, II (wheat germ oil, fresh juice of C. sativum, and A. vera in the ratio of 2:2:1), and III (wheat germ oil, fresh juice of C. sativum and A. vera in the ratio of 1:2:2) on alloxan-induced diabetic rats, after oral administration at doses of 1.0 ml/kg and 2.0 ml/kg. Blood samples were collected from the tail vein method at 0, 0.5, 1, 2, 4, 8, 12, and 24 h in normal rats and in diabetic rats at 0, 1, 3, 7, 15, and 30 days. Blood plasma glucose was estimated by the GOD/POD (glucose oxidase and peroxidase) method. The data were compared statistically by using the one-way ANOVA method followed by the Dunnett multiple component test. Statistical significance was set at P < 0.05.
  
  
  RESULTS
  The polyherbal preparation-I produced significant (P < 0.05) reduction in the blood glucose level of normal rats and polyherbal preparation-I, II, and III produced significant (P < 0.01) reduction in the blood glucose level of diabetic rats during 30-day study and compared with that of control and glibenclamide.
  
  
  CONCLUSIONS
  The polyherbal preparation-I showed a significant glucose lowering effect in normal rats and polyherbal preparation-I, II, and III in diabetic rats. This preparation is going to be promising antidiabetic preparation for masses; however, it requires further extensive studies in human beings.
authors.affiliation: Department of Pharmaceutical Sciences, Bhagwant University, Sikar Road, Ajmer, Rajasthan, India.
authors.forename: N
authors.initials: N
authors.lastname: Srivastava
authors.forename: G
authors.initials: G
authors.lastname: Tiwari
authors.forename: R
authors.initials: R
authors.lastname: Tiwari
authors.forename: L K
authors.initials: LK
authors.lastname: Bhati
authors.forename: Awani K
authors.initials: AK
authors.lastname: Rai