title: C/EBP homologous protein induces mesangial cell apoptosis under hyperglycemia.
abstract: Diabetes mellitus is known to cause kidney impairment; however, the mechanism remains elusive. The aim of this study was to investigate the role of C/EBP homologous protein (CHOP), an important protein in endoplasmic reticulum stress‑mediated mesangial cell apoptosis in hyperglycemia. Mesangial cells were cultured in normal (control group) and high glucose medium (high glucose group). TUNEL staining was performed to assess apoptotic cells in the groups. The expression of CHOP and caspase‑3 was also assayed by immunohistochemistry and western blot analysis. Following 24 h culture in high glucose medium, TUNEL‑positive cells were observed to be significantly increased (P<0.01). The expression of CHOP and caspase‑3 in mesangial cells was also found to be significantly enhanced under high glucose conditions compared with the normal group (P<0.01). The results indicate that CHOP mediates apoptosis in mesangial cells under hyperglycemia and may play a role in the development of diabetic nephropathy.
authors.affiliation: Department of Anatomy and Cell Biology, School of Medicine, Zhejiang                     University, Zhejiang 310058, P.R. China.
authors.forename: Daqiang
authors.initials: D
authors.lastname: He
authors.forename: Jianqiong
authors.initials: J
authors.lastname: Li
authors.forename: Jiayao
authors.initials: J
authors.lastname: Zhao
authors.forename: Jing
authors.initials: J
authors.lastname: Fei
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D005947:Glucose / Q000494:pharmacology; D006943:Hyperglycemia / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D050527:Mesangial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D051742:Transcription Factor CHOP / Q000378:metabolism*
chemical_list: D051742:Transcription Factor CHOP; D053148:Caspase 3; D005947:Glucose