title: The D3cpv Cameleon reports Ca²⁺ dynamics in plant mitochondria with similar kinetics of the YC3.6 Cameleon, but with a lower sensitivity.
abstract: Mitochondria are key organelles involved in many aspects of plant physiology and, their ability to generate specific Ca²⁺ signatures in response to abiotic and biotic stimuli has been reported as one of their roles. The recent identification of the mammalian mitochondrial Ca²⁺ uniporter opens a new research area in plant biology. To study the mitochondrial Ca²⁺ handling, it is essential to have a reliable probe. Here we have reported the generation of an Arabidopsis transgenic line expressing the genetically encoded probe Cameleon D3cpv targeted to mitochondria, and compared its properties with the already known Cameleon YC3.6.
authors.affiliation: Dipartimento di Biologia, Università degli Studi di Padova, Via U. Bassi, Padova, Italia.
authors.forename: G
authors.initials: G
authors.lastname: Loro
authors.forename: C
authors.initials: C
authors.lastname: Ruberti
authors.forename: M
authors.initials: M
authors.lastname: Zottini
authors.forename: A
authors.initials: A
authors.lastname: Costa
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D012680:Sensitivity and Specificity
chemical_list: D029681:Arabidopsis Proteins; D002135:Calcium-Binding Proteins; D005456:Fluorescent Dyes; D024101:Mitochondrial Proteins; D002118:Calcium