title: Tail-extension following the termination codon is critical for release of the nascent chain from membrane-bound ribosomes in a reticulocyte lysate cell-free system.
abstract: Nascent chain release from membrane-bound ribosomes by the termination codon was investigated using a cell-free translation system from rabbit supplemented with rough microsomal membrane vesicles. Chain release was extremely slow when mRNA ended with only the termination codon. Tail extension after the termination codon enhanced the release of the nascent chain. Release reached plateau levels with tail extension of 10 bases. This requirement was observed with all termination codons: TAA, TGA and TAG. Rapid release was also achieved by puromycin even in the absence of the extension. Efficient translation termination cannot be achieved in the presence of only a termination codon on the mRNA. Tail extension might be required for correct positioning of the termination codon in the ribosome and/or efficient recognition by release factors.
authors.affiliation: Graduate School of Life Science, University of Hyogo, Kouto, Ako-gun, Hyogo 678-1297, Japan.
authors.forename: Michiyo
authors.initials: M
authors.lastname: Takahara
authors.forename: Haruka
authors.initials: H
authors.lastname: Sakaue
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Onishi
authors.forename: Marifu
authors.initials: M
authors.lastname: Yamagishi
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Kida
authors.forename: Masao
authors.initials: M
authors.lastname: Sakaguchi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002462:Cell Membrane / Q000378:metabolism*; D002474:Cell-Free System / Q000378:metabolism; D018388:Codon, Terminator / Q000378:metabolism*; D004285:Dogs; D008969:Molecular Sequence Data; D010443:Peptide Chain Termination, Translational; D021381:Protein Transport; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D018388:Codon, Terminator