title: The effects of magnetic separation on cryopreserved bovine spermatozoa motility, viability and cryo-capacitation status.
abstract: Cryopreservation is a technique used to preserve cells for long-time storage. It is widely used in agriculture to store male gametes in liquid nitrogen. The aim of this study was to determine the optimum thawing temperature and time for samples subjected to annexin V magnetic-activated cell sorting (AnMACS) as the sperm preparation technique. Pooled semen samples from three ejaculates were divided into two groups. The treatment group was subjected both to AnMACS and to being cryopreserved, whilst the control group was cryopreserved directly without MACS. Post-thaw analysis was carried out for samples thawed at either 20°C for 13 s, 37°C for 30 s, 40°C for 7 s, 60°C for 6 s or 80°C for 5 s. Sperm kinematics, viability and capacitation status were determined for samples subjected to all thawing temperatures described. Results showed that thawing at 37°C for 13 s for MACS-processed samples was a superior option compared with other thawing procedures; there was a significant difference in P < 0.05 values for curvilinear velocity (VCL μm/s) and sperm straightness (STR %) when samples were thawed at 40°C for 7 s, with fewer capacitated spermatozoa (P < 0.05) when samples were thawed at 37°C for 30 s, 40°C for 7 s or 60°C for 6 s. Hence, we can speculate that the use of AnMACS as the sperm preparation technique can somehow enhance sperm cryosurvival rate after cryopreservation, however the fertilization potential of these cells has yet to be determined.
authors.affiliation: Department of Biology,Faculty of Applied Sciences,Universiti Teknologi MARA (UiTM),40450 Shah Alam,Selangor D.E.,Malaysia.
authors.forename: S S M
authors.initials: SS
authors.lastname: Faezah
authors.affiliation: Department of Biology,Faculty of Applied Sciences,Universiti Teknologi MARA (UiTM),40450 Shah Alam,Selangor D.E.,Malaysia.
authors.forename: F M Y
authors.initials: FM
authors.lastname: Zuraina
authors.affiliation: Department of Physiology,Medical Faculty,Universiti Kebangsaan Malaysia,Jalan Raja Muda Abdul Aziz,50300 Kuala Lumpur,Malaysia.
authors.forename: J H F
authors.initials: JH
authors.lastname: Farah
authors.affiliation: Forensic Science Programme,Faculty of Allied Health Sciences,Universiti Kebangsaan Malaysia,Jalan Raja Muda Abdul Aziz,50300 Kuala Lumpur,Malaysia.
authors.forename: O
authors.initials: O
authors.lastname: Khairul
authors.affiliation: Department of Biology,Faculty of Applied Sciences,Universiti Teknologi MARA (UiTM),40450 Shah Alam,Selangor D.E.,Malaysia.
authors.forename: N I
authors.initials: NI
authors.lastname: Hilwani
authors.affiliation: Department of Physiology,Medical Faculty,Universiti Kebangsaan Malaysia,Jalan Raja Muda Abdul Aziz,50300 Kuala Lumpur,Malaysia.
authors.forename: M I
authors.initials: MI
authors.lastname: Iswadi
authors.affiliation: Department of Biomedical Sciences,Faculty of Allied Health Sciences,Universiti Kebangsaan Malaysia,Jalan Raja Muda Abdul Aziz,50300 Kuala Lumpur,Malaysia.
authors.forename: C N
authors.initials: CN
authors.lastname: Fang
authors.affiliation: Institut Bioteknologi Veterinar Kebangsaan,Bukit Dinding,27000 Jerantut,Pahang,Malaysia.
authors.forename: I
authors.initials: I
authors.lastname: Zawawi
authors.affiliation: Agro-Biotechnology Institute,Malaysia,d/o MARDI Headquarters,43400 Serdang,Selangor D.E.,Malaysia.
authors.forename: O M
authors.initials: OM
authors.lastname: Abas
authors.affiliation: Department of Physiology,Medical Faculty,Universiti Kebangsaan Malaysia,Jalan Raja Muda Abdul Aziz,50300 Kuala Lumpur,Malaysia.
authors.forename: S I
authors.initials: SI
authors.lastname: Fatimah
mesh_terms: D000818:Animals; D002417:Cattle; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods*; D060328:Magnetic Phenomena; D008297:Male; D012662:Semen Preservation / Q000379:methods; D013075:Sperm Capacitation; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*; D013696:Temperature