title: Superparamagnetic iron oxide nanoparticle targeting of MSCs in vascular injury.
abstract: Vascular occlusion can result in fatal myocardial infarction, stroke or loss of limb in peripheral arterial disease. Interventional balloon angioplasty is a common first line procedure for vascular disease treatment, but long term success is limited by restenosis and neointimal hyperplasia. Cellular therapies have been proposed to mitigate these issues; however efficacy is low, in part due to poor cell retention. We show that magnetic targeting of mesenchymal stem cells gives rise to a 6-fold increase in cell retention following balloon angioplasty in a rabbit model using a clinically applicable permanent magnet. Cells labelled with superparamagnetic iron oxide nanoparticles exhibit no negative effects on cell viability, differentiation or secretion patterns. The increase in stem cell retention leads to a reduction in restenosis three weeks after cell delivery.
authors.affiliation: Centre for Advanced Biomedical Imaging, Department of Medicine and Institute of Child Health, University College London, London WC1E 6DD, UK. j.riegler@stanford.edu
authors.forename: Johannes
authors.initials: J
authors.lastname: Riegler
authors.forename: Aaron
authors.initials: A
authors.lastname: Liew
authors.forename: Sean O
authors.initials: SO
authors.lastname: Hynes
authors.forename: Daniel
authors.initials: D
authors.lastname: Ortega
authors.forename: Timothy
authors.initials: T
authors.lastname: O'Brien
authors.forename: Richard M
authors.initials: RM
authors.lastname: Day
authors.forename: Toby
authors.initials: T
authors.lastname: Richards
authors.forename: Faisal
authors.initials: F
authors.lastname: Sharif
authors.forename: Quentin A
authors.initials: QA
authors.lastname: Pankhurst
authors.forename: Mark F
authors.initials: MF
authors.lastname: Lythgoe
mesh_terms: D050156:Adipogenesis; D000818:Animals; D002448:Cell Adhesion; D020219:Chondrogenesis; D003911:Dextrans / Q000627:therapeutic use*; D004730:Endothelium, Vascular / Q000473:pathology; D005263:Femoral Artery / Q000473:pathology; D006083:Graft Occlusion, Vascular / Q000473:pathology; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D058185:Magnetite Nanoparticles / Q000627:therapeutic use*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000627:therapeutic use* / Q000648:ultrastructure; D010012:Osteogenesis; D011817:Rabbits; D013194:Staining and Labeling; D057772:Vascular System Injuries / Q000473:pathology / Q000601:surgery / Q000628:therapy*
chemical_list: D003911:Dextrans; D036341:Intercellular Signaling Peptides and Proteins; D058185:Magnetite Nanoparticles; C065507:ferumoxides