title: Fluorescence-based surface magnifying chromoendoscopy and optical coherence tomography endoscope.
abstract: A side-viewing, 2.3-mm diameter, surface magnifying chromoendoscopy-optical coherence tomography (SMC-OCT) endoscope has been designed for simultaneous, nondestructive surface fluorescence visualization and cross-sectional imaging. We apply this endoscope to in vivo examination of the mouse colon. A 30,000 element fiber bundle is combined with single mode fibers, for SMC and OCT imaging, respectively. The distal optics consist of a gradient-index lens and spacer to provide a 1× magnification at a working distance of 1.58 mm in air, necessary to image the sample through a 0.23-mm thick outer glass envelope, and an aluminized right-angle prism fixed to the distal end of the gradient-index lens assembly. The resulting 1∶1 imaging system is capable of 3.9-µm lateral and 2.3-µm axial resolution in the OCT channel, and 125-lp/mm resolution across a 0.70-mm field of view in the SMC channel. The endoscope can perform high contrast crypt visualization, molecular imaging, and cross-sectional imaging of colon microstructure.
authors.affiliation: The University of Arizona, College of Optical Sciences, 1630 E. University Boulevard, Tucson, AZ 85721, USA.
authors.forename: R Andrew
authors.initials: RA
authors.lastname: Wall
authors.forename: Jennifer K
authors.initials: JK
authors.lastname: Barton
mesh_terms: D000818:Animals; D003106:Colon / Q000033:anatomy & histology*; D020685:Colonoscopes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013382:Subtraction Technique / Q000295:instrumentation*; D041623:Tomography, Optical Coherence / Q000295:instrumentation*