title: Cyclic stretch reveals a mechanical role for intermediate filaments in a desminopathic cell model.
abstract: Mechanics is now recognized as crucial in cell function. To date, the mechanical properties of cells have been inferred from experiments which investigate the roles of actin and microtubules ignoring the intermediate filaments (IFs) contribution. Here, we analyse myoblasts behaviour in the context of myofibrillar myopathy resulting from p.D399Y desmin mutation which disorganizes the desmin IF network in muscle cells. We compare the response of myoblasts expressing either mutated or wild-type desmin to cyclic stretch. Cells are cultivated on supports submitted to periodic uniaxial stretch of 20% elongation amplitude and 0.3 Hz frequency. We show that during stretching cycles, cells expressing mutated desmin reduce their mean amplitude both for the elongation and spreading area compared to those expressing wild-type desmin. Even more unexpected, the reorientation angles are altered in the presence of p.D399Y desmin. Yet, at rest, the whole set of those parameters are similar for the two cell populations. Thus, we demonstrate that IFs affect the mechanical properties and the dynamics of cell reorientation. Since these processes are known due to actin cytoskeleton, these results suggest the IFs implication in mechanics signal transduction. Further studies may lead to better understanding of their contribution to this process.
authors.affiliation: Université Paris Sud, Paris 11, Laboratoire de Physique des solides, Bat 510-91405 Orsay Cedex, France.
authors.forename: E
authors.initials: E
authors.lastname: Leccia
authors.forename: S
authors.initials: S
authors.lastname: Batonnet-Pichon
authors.forename: A
authors.initials: A
authors.lastname: Tarze
authors.forename: V
authors.initials: V
authors.lastname: Bailleux
authors.forename: J
authors.initials: J
authors.lastname: Doucet
authors.forename: M
authors.initials: M
authors.lastname: Pelloux
authors.forename: F
authors.initials: F
authors.lastname: Delort
authors.forename: V
authors.initials: V
authors.lastname: Pizon
authors.forename: P
authors.initials: P
authors.lastname: Vicart
authors.forename: F
authors.initials: F
authors.lastname: Briki
mesh_terms: D002448:Cell Adhesion; D002478:Cells, Cultured; D003893:Desmin / Q000737:chemistry* / Q000235:genetics*; D006801:Humans; D007382:Intermediate Filaments / Q000235:genetics / Q000378:metabolism; D009135:Muscular Diseases / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology*; D009154:Mutation; D032446:Myoblasts / Q000378:metabolism*; D013314:Stress, Mechanical
chemical_list: D003893:Desmin