title: Fibroblast growth on micro- and nanopatterned surfaces prepared by a novel sol-gel phase separation method.
abstract: Physical characteristics of the growth substrate including nano- and microstructure play crucial role in determining the behaviour of the cells in a given biological context. To test the effect of varying the supporting surface structure on cell growth we applied a novel sol-gel phase separation-based method to prepare micro- and nanopatterned surfaces with round surface structure features. Variation in the size of structural elements was achieved by solvent variation and adjustment of sol concentration. Growth characteristics and morphology of primary human dermal fibroblasts were found to be significantly modulated by the microstructure of the substrate. The increase in the size of the structural elements, lead to increased inhibition of cell growth, altered morphology (increased cytoplasmic volume), enlarged cell shape, decrease in the number of filopodia) and enhancement of cell senescence. These effects are likely mediated by the decreased contact between the cell membrane and the growth substrate. However, in the case of large surface structural elements other factors like changes in the 3D topology of the cell's cytoplasm might also play a role.
authors.affiliation: Department of Physiology, University of Tartu, Tartu, Estonia. paula.reemann@ut.ee
authors.forename: Paula
authors.initials: P
authors.lastname: Reemann
authors.forename: Triin
authors.initials: T
authors.lastname: Kangur
authors.forename: Martin
authors.initials: M
authors.lastname: Pook
authors.forename: Madis
authors.initials: M
authors.lastname: Paalo
authors.forename: Liis
authors.initials: L
authors.lastname: Nurmis
authors.forename: Ilmar
authors.initials: I
authors.lastname: Kink
authors.forename: Orm
authors.initials: O
authors.lastname: Porosaar
authors.forename: Külli
authors.initials: K
authors.lastname: Kingo
authors.forename: Eero
authors.initials: E
authors.lastname: Vasar
authors.forename: Sulev
authors.initials: S
authors.lastname: Kõks
authors.forename: Viljar
authors.initials: V
authors.lastname: Jaks
authors.forename: Martin
authors.initials: M
authors.lastname: Järvekülg
mesh_terms: D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology*; D005782:Gels; D006801:Humans; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D013499:Surface Properties
chemical_list: D005782:Gels