title: Thrombin immobilization to methacrylic acid grafted poly(3-hydroxybutyrate) and its in vitro application.
abstract: Poly(3-hydroxybutyrate) is nontoxic and biodegradable, with good biocompatibility and potential support for long-term implants. For this reason, it is a good support for enzyme immobilization. Enzyme immobilization could not be done directly because poly(3-hydroxybutyrate) has no functional groups. Therefore, modification should be done for enzyme immobilization. In this study, methacrylic acid was graft polymerized to poly(3-hydroxybutyrate) and thrombin was immobilized to polymethacrylic acid grafted poly(3-hydroxybutyrate). In fact, graft polymerization of methacrylic acid to poly(3-hydroxybutyrate) and thrombin immobilization was a model study. Biomolecule immobilized poly(3-hydroxybutyrate) could be used as an implant. Thrombin was selected as a biomolecule for this model study and it was immobilized to methacrylic acid grafted poly(3-hydroxybutyrate). Then the developed product was used to stop bleeding.
authors.affiliation: Biochemistry Department, Faculty of Science, Ege University, Bornova-Izmir, Turkey. alper.akkaya@ege.edu.tr
authors.forename: Alper
authors.initials: A
authors.lastname: Akkaya
authors.forename: Nurdan
authors.initials: N
authors.lastname: Pazarlioglu
mesh_terms: D001777:Blood Coagulation / Q000187:drug effects; D057075:Enzyme Assays; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D006885:Hydroxybutyrates / Q000737:chemistry*; D007700:Kinetics; D008956:Models, Chemical; D011091:Polyesters / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013917:Thrombin / Q000737:chemistry* / Q000494:pharmacology; D014914:Whole Blood Coagulation Time; D000094122:Polyhydroxybutyrates
chemical_list: D004800:Enzymes, Immobilized; D006885:Hydroxybutyrates; D011091:Polyesters; D011109:Polymethacrylic Acids; D013917:Thrombin; C030613:polymethacrylic acid; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates