title: Participation of autophagy in lung ischemia-reperfusion injury in vivo.
abstract: BACKGROUND
  In response to stress, autophagy is activated and may present as a cell survival mechanism or lead to cell death. Although there has been some progress in studying the function of autophagy in the ischemia-reperfusion (I/R) injury of other organs, little is known about the role autophagy plays in lung I/R injury.
  
  
  METHODS
  A lung I/R injury model in rats was developed to explore the level of autophagy in lung I/R injury. An inhibitor of autophagy (3-methyladenine [3-MA]) was injected before ischemia to study the role autophagy plays in lung I/R injury.
  
  
  RESULTS
  The data indicated that the autophagic flux was elevated during the ischemia period, and was enhanced significantly during reperfusion. Inhibition of autophagy by 3-MA ameliorated lung I/R injury, as indicated by a reduced lung wet/dry ratio, myeloperoxidase activity, and malondialdehyde concentrations. 3-MA pretreatment also reduced cleaved caspase-3 and apoptosis in the lung, as indicated by the terminal deoxynucleotidyl transferase dUTP nick end labeling assay.
  
  
  CONCLUSIONS
  The results demonstrated that autophagy was involved in the lung I/R pathophysiological process, and it may be a scathing factor in lung I/R injury.
authors.affiliation: Department of Thoracic surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Jia-Shun
authors.initials: JS
authors.lastname: Wang
authors.forename: Zhi-Kun
authors.initials: ZK
authors.lastname: Zheng
authors.forename: Jian
authors.initials: J
authors.lastname: Tang
authors.forename: Kai
authors.initials: K
authors.lastname: Fan
authors.forename: Hui
authors.initials: H
authors.lastname: Guo
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Wang
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001343:Autophagy / Q000187:drug effects / Q000502:physiology*; D053148:Caspase 3 / Q000378:metabolism; D005260:Female; D008168:Lung / Q000098:blood supply*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000209:etiology*
chemical_list: C025946:3-methyladenine; D053148:Caspase 3; D000225:Adenine