title: Effects of Zizyphus lotus L. (Desf.) polyphenols on Jurkat cell signaling and proliferation.
abstract: We assessed the effects of Zizyphus lotus L. (Desf.) polyphenols (ZLP) on T-cell signaling and proliferation. Our results showed that ZLP exerted no effect on the increases in intracellular free calcium concentrations, [Ca(2+)]i, in human Jurkat T-cells. However, ZLP modulated the thapsigargin-induced increases in [Ca(2+)]i in these cells. ZLP treatment was found to decrease the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2). In addition, ZLP induced a rapid (t1/2=33s) and dose-dependent decrease in intracellular pH (pHi) in human Jurkat T-cells. Furthermore, ZLP significantly curtailed T-cell proliferation by diminishing their progression from S to G2/M phase of cell cycle, and the expression of interleukin-2 (IL-2) mRNA. Taken together, the results of the present study demonstrate that ZLP modulate cell signaling and exert immunosuppressive effects in human T-cells.
authors.affiliation: INSERM U866, Physiologie de la Nutrition & Toxicologie (NuTox), Université de Bourgogne, Dijon, France.
authors.forename: Souleymane
authors.initials: S
authors.lastname: Abdoul-Azize
authors.forename: Malika
authors.initials: M
authors.lastname: Bendahmane
authors.forename: Aziz
authors.initials: A
authors.lastname: Hichami
authors.forename: Gado
authors.initials: G
authors.lastname: Dramane
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Simonin
authors.forename: Chahid
authors.initials: C
authors.lastname: Benammar
authors.forename: Hassimi
authors.initials: H
authors.lastname: Sadou
authors.forename: Simon
authors.initials: S
authors.lastname: Akpona
authors.forename: Es-Saddik
authors.initials: ES
authors.lastname: El Boustani
authors.forename: Naim A
authors.initials: NA
authors.lastname: Khan
mesh_terms: D020013:Calcium Signaling / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005638:Fruit / Q000737:chemistry; D005786:Gene Expression Regulation; D006801:Humans; D007165:Immunosuppression Therapy; D007249:Inflammation / Q000276:immunology*; D007376:Interleukin-2 / Q000235:genetics / Q000378:metabolism*; D019169:Jurkat Cells; D059808:Polyphenols / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D019284:Thapsigargin / Q000276:immunology; D031957:Ziziphus / Q000737:chemistry*
chemical_list: D007376:Interleukin-2; D059808:Polyphenols; D012333:RNA, Messenger; D019284:Thapsigargin; D048049:Extracellular Signal-Regulated MAP Kinases