title: Sequence-specific backbone ¹H, ¹³C and ¹⁵N assignments of the catalytic domain of the Escherichia coli protein tyrosine kinase, Wzc.
abstract: Protein tyrosine kinases in bacteria are structurally and functionally distinct from their eukaryotic counterparts. The largest family of bacterial tyrosine kinases, the BY-kinase family, is highly conserved in Gram-negative and Gram-positive species, and plays a central role in biofilm and capsule formation. In Escherichia coli the BY-kinase, Wzc, is a critical component of the machinery responsible for the synthesis and export of the exo-polysaccharide colanic acid, a key constituent of biofilms. Here we present the main-chain (1)H(N), (15)N, (13)C' and (13)Cα, side-chain (13)Cβ resonance assignments for a construct that encodes the entire 274-residue cytosolic catalytic domain of Wzc.
authors.affiliation: Department of Chemistry, The City College of New York, 160 Convent Avenue, New York, NY, 10031, USA.
authors.forename: Deniz B
authors.initials: DB
authors.lastname: Temel
authors.forename: Kaushik
authors.initials: K
authors.lastname: Dutta
authors.forename: Ranajeet
authors.initials: R
authors.lastname: Ghose
mesh_terms: D000595:Amino Acid Sequence; D002247:Carbon Isotopes; D020134:Catalytic Domain; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D006859:Hydrogen; D008565:Membrane Proteins / Q000737:chemistry*; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D011505:Protein-Tyrosine Kinases / Q000737:chemistry*
chemical_list: D002247:Carbon Isotopes; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D009587:Nitrogen Isotopes; D006859:Hydrogen; D011505:Protein-Tyrosine Kinases; C499615:wzc protein, E coli