title: Expression and cellular distribution of INHA and INHB before and after in vitro cultivation of porcine oocytes isolated from follicles of different size.
abstract: Cumulus-oocyte-complexes (COCs) were collected from small (<3 mm), medium (3-5 mm), and large (>5 mm) porcine follicles, and the INHA and INHB expression and cellular localization were studied. Developmentally competent (BCB+) COCs were cultured for 44 h. Samples of mRNA were isolated before and after in vitro maturation (IVM) from oocytes collected from follicles of different size for RQ-PCR assay. The INHA and INHB protein distribution within the oocytes was observed by confocal microscopy. INHA mRNA expression was increased in oocytes from large compared to medium and small follicles before IVM (P < 0.001), and to oocytes of small follicles after IVM (P < 0.001). The INHB expression was not different before IVM, but the IHNB mRNA level was gradually higher in oocytes from large follicles after IVM (P < 0.01). INHA was not differently expressed before IVM; however, in large follicle oocytes the protein was distributed in the peripheral area of the cytoplasm; in oocytes from small follicles it was in the entire cytoplasm. After IVM, INHA was strongly expressed in oocytes from small follicles and distributed particularly in the zona pellucida (ZP). Similarly and both before and after IVM, INHB protein was highly expressed in small follicle oocytes and within the cytoplasm. In summary, INHs can be recognized as a marker of porcine oocyte quality.
authors.affiliation: Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland. etok@op.pl
authors.forename: Bartosz
authors.initials: B
authors.lastname: Kempisty
authors.forename: Marta
authors.initials: M
authors.lastname: Jackowska
authors.forename: Magdalena
authors.initials: M
authors.lastname: Woźna
authors.forename: Paweł
authors.initials: P
authors.lastname: Antosik
authors.forename: Hanna
authors.initials: H
authors.lastname: Piotrowska
authors.forename: Piotr
authors.initials: P
authors.lastname: Zawierucha
authors.forename: Dorota
authors.initials: D
authors.lastname: Bukowska
authors.forename: Jędrzej M
authors.initials: JM
authors.lastname: Jaśkowski
authors.forename: Michał
authors.initials: M
authors.lastname: Nowicki
authors.forename: Klaus P
authors.initials: KP
authors.lastname: Brüssow
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation; D005260:Female; D005786:Gene Expression Regulation; D028322:Inhibin-beta Subunits / Q000235:genetics / Q000378:metabolism*; D007265:Inhibins / Q000235:genetics / Q000378:metabolism*; D018613:Microscopy, Confocal; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism*; D009929:Organ Size; D006080:Ovarian Follicle / Q000033:anatomy & histology* / Q000166:cytology*; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034421:Sus scrofa
chemical_list: D012333:RNA, Messenger; C094377:inhibin-alpha subunit; D007265:Inhibins; D028322:Inhibin-beta Subunits