title: ¹H, ¹⁵N and ¹³C resonance assignments of PpdD, a type IV pilin from enterohemorrhagic Escherichia coli.
abstract: Bacterial type 4 pili (T4P) are long flexible fibers involved in adhesion, DNA uptake, phage transduction, aggregation and a flagella-independent movement called "twitching motility". T4P comprise thousands of copies of the major pilin subunit, which is initially inserted in the plasma membrane, processed and assembled into dynamic helical filaments. T4P are crucial for host colonization and virulence of many Gram-negative bacteria. In enterohemorrhagic Escherichia coli the T4P, called hemorrhagic coli pili (HCP) promote cell adhesion, motility, biofilm formation and signaling. To understand the mechanism of HCP assembly and function, we analyzed the structure of the major subunit prepilin peptidase-dependent protein D (PpdD) (also called HcpA), a 15 kDa pilin with two potential disulfide bonds. Here we present the (1)H, (15)N and (13)C backbone and side chain resonance assignments of the C-terminal globular domain of PpdD as a first step to its structural determination.
authors.affiliation: Unité de RMN des Biomolécules, Département de Biologie Structurale et Chimie, Institut Pasteur, 28, rue du Dr Roux, 75724, Paris Cedex 15, France, gamorim@cnrmn.bioqmed.ufrj.br.
authors.forename: Gisele C
authors.initials: GC
authors.lastname: Amorim
authors.forename: David A
authors.initials: DA
authors.lastname: Cisneros
authors.forename: Muriel
authors.initials: M
authors.lastname: Delepierre
authors.forename: Olivera
authors.initials: O
authors.lastname: Francetic
authors.forename: Nadia
authors.initials: N
authors.lastname: Izadi-Pruneyre
mesh_terms: D002247:Carbon Isotopes; D054324:Enterohemorrhagic Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D036781:Fimbriae Proteins / Q000737:chemistry*; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D006859:Hydrogen; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D002247:Carbon Isotopes; D029968:Escherichia coli Proteins; D009587:Nitrogen Isotopes; C000592919:PpdD protein, E coli; D036781:Fimbriae Proteins; D006859:Hydrogen