title: [Protective effect and mechanism of Ecliptae Herba on cigarette smoke extract-induced cytotoxicity of NHBE cells].
abstract: OBJECTIVE
  To investigate the protective effect and mechanism of Ecliptae Herba extract on cigarette smoke extract-induced cytotoxicity.
  
  
  METHODS
  The effect of Ecliptae Herba extract on CSE-induced NHBE cell proliferation was detected by MTT assay. GSH content was determined by DTNB colorimetry. GST activity was measured by CDNB colorimetric assay. NQO1 activity was detected by NADPH and DCIP. The protein expression was determined by Western blot assay.
  
  
  RESULTS
  Ecliptae Herba extract reduced CSE's inhibitory effect on NHBE cells, recover the decrease in intracellular GSH caused by CSE and reduce the CSE-induced activity of GST and NQO1 and NQO1 protein expression.
  
  
  CONCLUSIONS
  Ecliptae Herba extract can reduce CSE-induced injury on NHBE cells, which may be related to phase II detoxification enzymes.
authors.affiliation: Department of Life Sciences, Anhui University, Hefei 230039, China. lwb870526@yahoo.cn
authors.forename: Wenbo
authors.initials: W
authors.lastname: Liu
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Tan
authors.forename: Hanyan
authors.initials: H
authors.lastname: Sun
authors.forename: Houcai
authors.initials: H
authors.lastname: Huang
authors.forename: Ping
authors.initials: P
authors.lastname: Jin
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Jia
authors.forename: Siming
authors.initials: S
authors.lastname: Yu
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D036601:Eclipta / Q000737:chemistry*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D012906:Smoke / Q000032:analysis; D012907:Smoking / Q000009:adverse effects*; D014026:Nicotiana / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D020011:Protective Agents; D012906:Smoke; D016660:NAD(P)H Dehydrogenase (Quinone); C447479:NQO1 protein, human