title: Efficient synthesis of (+)-cis-12-oxo-phytodienoic acid by an in vitro enzymatic reaction.
abstract: 12-Oxo-phytodienoic acid (OPDA) is an important metabolite on the octadecanoid pathway of plants. This study shows an efficient in vitro synthesis of (+)-cis-OPDA by using a flaxseed extract and an allene oxide cyclase. The OPDA yield of the reaction in this study was almost 7-fold higher than that in the conventional reaction with the flaxseed extract.
authors.affiliation: Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
authors.forename: Akiyuki
authors.initials: A
authors.lastname: Kajiwara
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Abe
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hashimoto
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Matsuura
authors.forename: Kosaku
authors.initials: K
authors.lastname: Takahashi
mesh_terms: D060326:Chemistry Techniques, Synthetic; D005231:Fatty Acids, Unsaturated / Q000138:chemical synthesis* / Q000737:chemistry*; D019597:Flax / Q000737:chemistry; D019746:Intramolecular Oxidoreductases / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry
chemical_list: D005231:Fatty Acids, Unsaturated; D010936:Plant Extracts; C025999:12-oxophytodienoic acid; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase