title: Optimization of selenylation conditions for Chinese angelica polysaccharide based on immune-enhancing activity.
abstract: Chinese angelica polysaccharide (CAP) was extracted by water decoction and ethanol precipitation, purified through eliminating protein by Sevage method and column chromatography of Sephadex G-200, then selenizingly modified by nitric acid-sodium selenite method according to L(9)(3(4)) orthogonal design of three-factors, the usage amount of sodium selenite, reaction temperature and reaction time, at three level to obtain nine selenizing CAPs, sCAP(1)-sCAP(9). Their effects on chicken peripheral lymphocytes proliferation in vitro were compared by MTT assay taking the non-modified CAP as control. The results showed that selenylation modification could significantly enhance the immune-enhancing activity of CAP, sCAP(2) presented best effect and the optimal modification conditions were 200mg of sodium selenite for 500 mg of CAP, the reaction temperature of 70 °C and the reaction time of 6 h.
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Qin
authors.forename: Jin
authors.initials: J
authors.lastname: Chen
authors.forename: Deyun
authors.initials: D
authors.lastname: Wang
authors.forename: Yuanliang
authors.initials: Y
authors.lastname: Hu
authors.forename: Mi
authors.initials: M
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: The Luong
authors.initials: TL
authors.lastname: Nguyen
authors.forename: Cui
authors.initials: C
authors.lastname: Liu
authors.forename: Xu
authors.initials: X
authors.lastname: Liu
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology; D029971:Angelica sinensis; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002645:Chickens / Q000276:immunology; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D017942:Nitric Acid / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018038:Sodium Selenite / Q000737:chemistry*
chemical_list: D000276:Adjuvants, Immunologic; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D017942:Nitric Acid; C044613:angelicae sinensis extract; D018038:Sodium Selenite