title: Recombinant OmpL1 protein as a diagnostic antigen for the detection of canine leptospirosis.
abstract: Microscopic agglutination test (MAT) is the standard method for the diagnosis of leptospirosis, which is laborious and the interpretation of the results is subjective. The present work describes the use of recombinant-based IgG ELISA for the serodiagnosis of leptospirosis. We used recombinant outer membrane protein OmpL1 as an antigen for conducting IgG enzyme-linked immunosorbent assay (ELISA). A total of 475 canine serum samples were subjected to IgG ELISA; 294 sera were positive to ELISA, while 283 were positive to MAT. All samples that were positive to MAT were positive to ELISA also, however, few samples which were negative to MAT were positive to ELISA, which suggested that recombinant-based IgG ELISA showed 100 % sensitivity when compared to MAT. Thus, this present study showed that recombinant OmpL1-based IgG ELISA appears to be a better alternative to MAT for the diagnosis of leptospirosis and rOmpL1 protein could be used as a potential diagnostic antigen in different assay formats for leptospirosis.
authors.affiliation: Department of Animal Biotechnology, Madras Veterinary College, TANUVAS, Chennai 600 007, Tamil Nadu, India.
authors.forename: M
authors.initials: M
authors.lastname: Subathra
authors.forename: T M A
authors.initials: TM
authors.lastname: Senthilkumar
authors.forename: P
authors.initials: P
authors.lastname: Ramadass
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D004283:Dog Diseases / Q000175:diagnosis* / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007922:Leptospirosis / Q000175:diagnosis* / Q000276:immunology / Q000662:veterinary*; D011994:Recombinant Proteins / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D001425:Bacterial Outer Membrane Proteins; D011994:Recombinant Proteins; C081904:ompL1 protein, Leptospira