title: Differentially  expressed genes implicated in embryo abortion of mango identified by  suppression subtractive hybridization.
abstract: Embryo  abortion in mango severely damages mango production worldwide. The mechanisms  by which the mango embryos abort have long been an intriguing question. We used  subtractive suppression hybridization to investigate the differentially  expressed genes involved in this process. We generated 2 cDNA libraries from  normal seed and aborted seed embryos of mango cultivar 'Jinhuang'. One thousand  five hundred and seventy-two high-quality expressed sequence tags (ESTs) were  obtained, with 1092 from the normal seed tester library and 480 from the  aborted seed tester library. These ESTs were assembled into 783 unigenes,  including 147 contigs and 636 singletons in contigs; 297 singletons in gene  ontology (GO) indicated coverage of a broad range of GO categories. Seven  candidate genes from different categories were selected for semi-quantitative  PCR analysis, and their possible functions in embryo abortion are discussed.  These data provide new insight into the genetic regulation of embryo abortion  in mango and may aid in further identification of novel genes and their functions.
authors.affiliation: College of Horticulture, Northwest A&F University, Yangling, China.
authors.forename: J H
authors.initials: JH
authors.lastname: He
authors.forename: F W
authors.initials: FW
authors.lastname: Ma
authors.forename: Y Y
authors.initials: YY
authors.lastname: Chen
authors.forename: H R
authors.initials: HR
authors.lastname: Shu
mesh_terms: D001483:Base Sequence; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D031022:Mangifera / Q000196:embryology* / Q000235:genetics*; D058977:Molecular Sequence Annotation; D009693:Nucleic Acid Hybridization / Q000379:methods*; D051479:Plant Infertility / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000235:genetics*