title: In vitro cytocompatibility evaluation of alginate dialdehyde for biological tissue fixation.
abstract: Biological tissues must be chemically fixed before they can be implanted in humans, due to the immediate degradation and presence of antigenicity of naturally derived tissues. To provide a crosslinking reagent which is cytocompatible and may prepare biocompatible fixed tissues, a novel crosslinking agent, alginate dialdehyde (ADA), was employed to fix biological tissues by our group. The study was to evaluate the cytocompatibility of ADA for biological tissue fixation. Glutaraldehyde and genipin counterparts were used as controls. The result suggested that the cytotoxicity of ADA was significantly lower than that of glutaraldehyde and genipin. Additionally, in the evaluation of cytotoxicity of fixed tissue itself and the residues, as well as the cell adhesion property, ADA-fixed tissue was significantly superior to its glutaraldehyde counterpart and comparable to its genipin counterpart. The results obtained in this study demonstrate that ADA is a cytocompatible crosslinking reagent for biological tissue fixation.
authors.affiliation: College of Polymer Science and Engineering, Sichuan University, Chengdu, Sichuan Province 610065, PR China.
authors.forename: Yuanting
authors.initials: Y
authors.lastname: Xu
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Xixun
authors.initials: X
authors.lastname: Yu
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Gu
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Huang
authors.forename: Hong
authors.initials: H
authors.lastname: Peng
mesh_terms: D000464:Alginates / Q000737:chemistry / Q000494:pharmacology; D001672:Biocompatible Materials; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000494:pharmacology; D020723:Glucuronic Acid / Q000737:chemistry / Q000494:pharmacology; D005976:Glutaral / Q000494:pharmacology; D006603:Hexuronic Acids / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D039823:Iridoids / Q000494:pharmacology; D016707:Tissue Fixation
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D006603:Hexuronic Acids; D039823:Iridoids; D020723:Glucuronic Acid; D002482:Cellulose; C058684:2,3-dialdehydocellulose; C007834:genipin; D005976:Glutaral