title: A new triterpenoid from Alisma orientale and their antibacterial effect.
abstract: A new triterpenoid, named alisol Q 23-acetate, as well as fourteen known terpenes, alisol B 23-acetate (2), alisol B (3), alismol (4), 10-O-methyl-alismoxide (5), alismoxide (6), 11-deoxyalisol C (7), 13β,17β-epoxyalisol B 23-acetate (8), 4β,12-dihydroxyguaian-6,10-diene (9), alisol C 23-acetate (10), alisolide (11), 16β-methoxyalisol B monoacetate (12), alisol A (13), 16β-hydroxyalisol B 23-acetate (14), alisol A 24-acetate (15) were isolated from the rhizomes of Alisma orientale. The structures of compounds (1-15) were identified based on 1D and 2D NMR, including (1)H-(1)H COSY, HSQC, HMBC and NOESY spectroscopic analyses. Among these isolates, antibacterial effect of compounds 2, 3, 10, and 15, major constituents of A. orientale was examined. The MIC values of compounds 2, 10, and 15 were 5-10 βg/mL against eight antibiotic resistant strains, which were lower than those from the positive controls (MICs of chloramphenicol and ampicillin were 5-80 μg/mL). Therefore, compounds 2, 10 and 15 exhibited the potent antibacterial activity.
authors.affiliation: College of Pharmacy, Chosun University, Gwangju 501-759, Korea.
authors.forename: Hong-Guang
authors.initials: HG
authors.lastname: Jin
authors.forename: Qinglong
authors.initials: Q
authors.lastname: Jin
authors.forename: A
authors.initials: A
authors.lastname: Ryun Kim
authors.forename: Hyemin
authors.initials: H
authors.lastname: Choi
authors.forename: Je Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Yeong Shik
authors.initials: YS
authors.lastname: Kim
authors.forename: Dong Gun
authors.initials: DG
authors.lastname: Lee
authors.forename: Eun-Rhan
authors.initials: ER
authors.lastname: Woo
mesh_terms: D034902:Alisma / Q000737:chemistry*; D000667:Ampicillin / Q000008:administration & dosage / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D002701:Chloramphenicol / Q000008:administration & dosage / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D027343:Rhizome; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D014315:Triterpenes; D002701:Chloramphenicol; D000667:Ampicillin