title: Sulfated modification of the polysaccharide from Cordyceps_gunnii mycelia and its biological activities.
abstract: A chemically new sulfated polysaccharide (SPS50) was prepared from the water soluble polysaccharide (PS50), isolated from Cordyceps_gunnii mycelia, by concentrated sulfuric acid method. The yield of crude SPS50 was 62.34% and its specific rotation was [α](D)(20)=-36.75°. The structural characteristics of this chemically sulfated polysaccharide were determined based on the infrared analysis (IR), high performance liquid chromatography (HPLC) and sodium dodecyl sulfate polyacrylamide gel electropheresis (SDS-PAGE). Its biological properties including anti-oxidant and anti-tumor activities were also investigated. The results showed that the anti-oxidant capacity of SPS50 was not as good as SP50 and the anti-tumor activity of SPS50 was much better than PS50. SPS50 showed evident growth inhibition on K562 cells. The tumor inhibition ratio of SPS50 against K562 cells was 69.92%.
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China. zhyuanzhu@tust.edu.cn
authors.forename: Zhen-Yuan
authors.initials: ZY
authors.lastname: Zhu
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Chuan-Ling
authors.initials: CL
authors.lastname: Si
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lv
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Li
authors.forename: Guo-Ling
authors.initials: GL
authors.lastname: Dong
authors.forename: An-Jun
authors.initials: AJ
authors.lastname: Liu
authors.forename: Yong-Min
authors.initials: YM
authors.lastname: Zhang
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D032661:Cordyceps / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D020014:K562 Cells / Q000187:drug effects; D025282:Mycelium / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013431:Sulfates / Q000737:chemistry; D013481:Superoxides / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D011134:Polysaccharides; D013431:Sulfates; D013481:Superoxides