title: Cloning and expression analysis of ten genes associated with picrosides biosynthesis in Picrorhiza kurrooa.
abstract: Picrorhiza kurrooa Royle ex Benth. is an economically important medicinal plant known to yield picrosides which have high medicinal value. Picroside I and picroside II are major picrosides associated with various bioactivities. The present work analyzed the expression of various genes of the picrosides biosynthesis pathway in different tissues of the plant in relation to the picrosides content. Eight full-length cDNA sequences namely, 1-deoxy-d-xylulose-5-phosphate synthase (2.317 kb), 1-deoxy-d-xylulose-5-phosphate reductoisomerase (1.767 kb), 4-diphosphocytidyl-2-C-methyl-d-erythritol kinase (1.674 kb), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (1.701 kb), acetyl-CoA acetyltransferase (1.545 kb), 3-hydroxy-3-methylglutaryl coenzyme A reductase (2.241 kb), isopentenyl pyrophosphate isomerase (987 bp) and geranyl diphosphate synthase (1.434 kb), were cloned to full-length followed by expression analysis of ten genes vis-à-vis picrosides content analysis. There is maximum accumulation of picrosides in leaf tissue followed by the rhizome and root, and a similar pattern of expression was found in all the ten genes. The genes responded to the modulators of the picrosides biosynthesis. Picrosides accumulation was enhanced by application of hydrogen peroxide and abscisic acid, whereas methyl jasmonate and salicylic acid treatment decreased the content.
authors.affiliation: Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology (Council of Scientific and Industrial Research), P.O. Box No. 6, Palampur 176 061, Himachal Pradesh, India. harsharan78@gmail.com
authors.forename: Harsharan
authors.initials: H
authors.lastname: Singh
authors.forename: Parul
authors.initials: P
authors.lastname: Gahlan
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology / Q000502:physiology; D000085:Acetates / Q000494:pharmacology; D053898:Biosynthetic Pathways / Q000235:genetics; D002934:Cinnamates / Q000378:metabolism*; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000494:pharmacology; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006861:Hydrogen Peroxide / Q000494:pharmacology; D057888:Iridoid Glucosides / Q000378:metabolism*; D054883:Oxylipins / Q000494:pharmacology; D032261:Picrorhiza / Q000235:genetics* / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology / Q000502:physiology; D018515:Plant Leaves / Q000235:genetics* / Q000378:metabolism; D010946:Plants, Medicinal / Q000235:genetics / Q000378:metabolism; D027343:Rhizome / Q000235:genetics / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology
chemical_list: D000085:Acetates; D002934:Cinnamates; D003517:Cyclopentanes; D057888:Iridoid Glucosides; D054883:Oxylipins; D010937:Plant Growth Regulators; C109270:picroside I; C416837:picroside II; D000040:Abscisic Acid; C072239:methyl jasmonate; D006861:Hydrogen Peroxide; D020156:Salicylic Acid