title: [Simultaneous determination of four kinds of furocoumarins in cosmetics by high performance liquid chromatography].
abstract: OBJECTIVE
  A high performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of 4 furocoumarins, including 8-methoxycoumarin, 5-methoxycoumarin, trioxsalen and imperatorin in cosmetics.
  
  
  METHODS
  The separation was carried on Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) by gradient elution with methanol-water as mobile phase at a flow rate of 1.0 ml/min and column temperature of 30 degrees C. The analysis of 4 furocoumarins was performed by HPLC with diodearray detector at the wavelength of 248 nm.
  
  
  RESULTS
  Identification of 4 furocoumarins was achieved by retention time, and quantification analysis was performed by the external standard method. The limits of quantitation (LOQ) for 4 furocoumarins were 0.54, 0.52, 0.58 and 0.50 microg/g, respectively. The method showed a good linearity in the range of 0.05-10 microg/ml with correlation coefficients more than 0.999. The mean recoveries at spiked levels were in the range of 89.9%-105.2% with RSDs of 0.20%-1.08%.
  
  
  CONCLUSIONS
  The method was accurate and simple, and was suitable for the determination of 4 furocoumarins in cosmetics.
authors.affiliation: State Food and Drug Administration Review of Health Food Center, Beijing 100070, China.
authors.forename: Baojun
authors.initials: B
authors.lastname: Liu
authors.forename: Lili
authors.initials: L
authors.lastname: Yan
authors.forename: Qiuyue
authors.initials: Q
authors.lastname: Li
authors.forename: Jinfang
authors.initials: J
authors.lastname: Wang
authors.forename: Yue
authors.initials: Y
authors.lastname: Shi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003358:Cosmetics / Q000737:chemistry*; D003375:Coumestrol / Q000031:analogs & derivatives / Q000032:analysis; D011564:Furocoumarins / Q000032:analysis*; D017319:Photosensitizing Agents / Q000032:analysis*; D014307:Trioxsalen / Q000032:analysis
chemical_list: C545723:8-methoxycoumestrol; D003358:Cosmetics; D011564:Furocoumarins; D017319:Photosensitizing Agents; C031534:imperatorin; D003375:Coumestrol; D014307:Trioxsalen