title: Eps8 promotes cellular growth of human malignant gliomas.
abstract: Eps8 was initially identified as a substrate of the epidermal growth factor             receptor. Overexpression of Eps8 leads to increased mitogenic signaling and malignant             transformation. However, little is known concerning the importance of Eps8 in             human gliomas. In this study, we found that Eps8 was overexpressed in 56.6% of             human gliomas (WHO grades III and IV) compared with adjacent normal brain tissues             by immunohistochemical analysis. The U251 human glioma cell line stably expressing             Eps8 was established by G418 screening, and the ectopic expression of Eps8 enhanced             U251 glioma cell growth and survival by cell survival, MTT and liquid colony formation             assays. By contrast, the lentiviral expression of Eps8 siRNA in SHG-44 cells resulted             in a significant reduction in cellular growth and proliferation. Furthermore,             Eps8 modulated the levels of phosphorylated extracellular signal-regulated protein             kinase (ERK), phosphorylated serine-threonine protein kinase Akt and β-catenin             expression in glioma cell lines and tissues. These results suggest that Eps8 is             overexpressed in human gliomas, and affects glioma cell growth possibly by regulating             ERK and Akt/β-catenin signaling. Therefore, Eps8 may represent a novel potential             target in human glioma therapy.
authors.affiliation: Key Laboratory of Protein Chemistry and Developmental Biology of the State Education Ministry of China, College of Life Science, Hunan Normal University, Changsha, PR China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ding
authors.forename: Fangliang
authors.initials: F
authors.lastname: Zhou
authors.forename: Fangmei
authors.initials: F
authors.lastname: Wang
authors.forename: Zijian
authors.initials: Z
authors.lastname: Yang
authors.forename: Chang
authors.initials: C
authors.lastname: Zhou
authors.forename: Jianlin
authors.initials: J
authors.lastname: Zhou
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Junmei
authors.initials: J
authors.lastname: Yang
authors.forename: Guangwei
authors.initials: G
authors.lastname: Wang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wei
authors.forename: Xiang
authors.initials: X
authors.lastname: Hu
authors.forename: Shuanglin
authors.initials: S
authors.lastname: Xiang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D000368:Aged; D001921:Brain / Q000378:metabolism; D001932:Brain Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D005822:Genetic Vectors; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D016086:Lentivirus; D008297:Male; D008875:Middle Aged; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering; D014162:Transfection; D051176:beta Catenin / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C489989:EPS8 protein, human; D034741:RNA, Small Interfering; D051176:beta Catenin; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases