title: Development of a liquid-phase blocking ELISA based on foot-and-mouth disease virus empty capsid antigen for seromonitoring vaccinated animals.
abstract: In foot-and-mouth disease (FMD) control programme, liquid-phase blocking ELISA (LPBE) is widely used to assay vaccine-induced seroconversion. Currently, the assay utilizes inactivated FMD virus antigen for the detection of antibodies in serum samples. To develop a non-infectious substitute for the antigen in LPBE, we expressed the structural polypeptide of FMDV (serotype A) using a baculovirus expression system, and show that inclusion of viral 3C with reduced protease activity resulted in a higher yield of structural proteins. Structural proteins expressed in insect cells assembled into empty virus-like particles (VLPs) and showed antigenicity comparable to chemically inactivated FMDV. Screening of serum samples from FMD-vaccinated cattle showed that the test performance of VLP-LPBE had a correlation of 0.89 with conventional inactivated virus antigen LPBE. The VLP-LPBE developed here demonstrates the diagnostic application of recombinant FMDV VLPs in monitoring seroconversion following FMD vaccination.
authors.affiliation: Indian Veterinary Research Institute, Hebbal, Bangalore 560024, India.
authors.forename: S H
authors.initials: SH
authors.lastname: Basagoudanavar
authors.forename: M
authors.initials: M
authors.lastname: Hosamani
authors.forename: R P
authors.initials: RP
authors.lastname: Tamil Selvan
authors.forename: B P
authors.initials: BP
authors.lastname: Sreenivasa
authors.forename: P
authors.initials: P
authors.lastname: Saravanan
authors.forename: B K
authors.initials: BK
authors.lastname: Chandrasekhar Sagar
authors.forename: R
authors.initials: R
authors.lastname: Venkataramanan
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology; D036022:Capsid Proteins / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000517:prevention & control; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D012698:Serologic Tests / Q000379:methods; D014730:Veterinary Medicine / Q000379:methods*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D011994:Recombinant Proteins; D014765:Viral Vaccines