title: Poly(C)-binding protein 1, a novel N(pro)-interacting protein involved in classical swine fever virus growth.
abstract: N(pro) is a multifunctional autoprotease unique to pestiviruses. The interacting partners of the N(pro) protein of classical swine fever virus (CSFV), a swine pestivirus, have been insufficiently defined. Using a yeast two-hybrid screen, we identified poly(C)-binding protein 1 (PCBP1) as a novel interacting partner of the CSFV N(pro) protein and confirmed this by coimmunoprecipitation, glutathione S-transferase (GST) pulldown, and confocal assays. Knockdown of PCBP1 by small interfering RNA suppressed CSFV growth, while overexpression of PCBP1 promoted CSFV growth. Furthermore, we showed that type I interferon was downregulated by PCBP1, as well as N(pro). Our results suggest that cellular PCBP1 positively modulates CSFV growth.
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Su
authors.initials: S
authors.lastname: Li
authors.forename: Yuan
authors.initials: Y
authors.lastname: Sun
authors.forename: Hong
authors.initials: H
authors.lastname: Dong
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Li
authors.forename: Bibo
authors.initials: B
authors.lastname: Zhao
authors.forename: Dongwei
authors.initials: D
authors.lastname: Guo
authors.forename: Changjiang
authors.initials: C
authors.lastname: Weng
authors.forename: Hua-Ji
authors.initials: HJ
authors.lastname: Qiu
mesh_terms: D000818:Animals; D002460:Cell Line; D002498:Centrifugation; D006692:Classical Swine Fever Virus / Q000254:growth & development*; D010450:Endopeptidases / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D054884:Host-Pathogen Interactions; D006801:Humans; D047468:Immunoprecipitation; D018613:Microscopy, Confocal; D011485:Protein Binding; D025941:Protein Interaction Mapping; D034741:RNA, Small Interfering / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D013552:Swine; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D014764:Viral Proteins; D010450:Endopeptidases; C505229:N(pro) protein, swine fever virus