title: Green tea extract (Camellia sinensis) fermented by Lactobacillus fermentum attenuates alcohol-induced liver damage.
abstract: Here, the impact of an extract derived from green tea (Camellia sinensis) and fermentation with Lactobacilli fermentum strain OCS19 was explored with acute alcohol-induced liver damage. The study employed the HepG2 hepatic cell line and an in vivo murine model of liver damage. L. fermentum-fermented green tea extract (FGTE) was found to possess pronounced alcohol metabolizing enzyme activity. It significantly enhanced the cell viability of HepG2 cells following of them exposure, to ethanol (p<0.05) as compared with an extract derived from Hovenia dulcis, a positive control that is known for its action as an alcohol antagonist. Our in vivo studies indicated that prior administration of FGTE to alcohol-exposed mice significantly prevented subsequent increases in blood alcohol concentration (p<0.05), in addition to the induction of serum alanine aminotransferase (ALT) and triglycerides (p<0.05). Furthermore, the activity of hepatic alcohol dehydrogenase (ADH) and its mRNA expression level both increased in the livers of mice treated with FGTE, similarly to the H. dulcis-treated group. Taken together, these results may suggest that green tea extract coupled with L. fermentum fermentation attenuates the risk of ethanol-induced liver damage.
authors.affiliation: Division of Food Bioscience and Technology, Korea University, Seoul, Korea.
authors.forename: Jong Ho
authors.initials: JH
authors.lastname: Park
authors.forename: Younghoon
authors.initials: Y
authors.lastname: Kim
authors.forename: Sae Hun
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000438:Alcohols / Q000633:toxicity*; D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D000818:Animals; D028241:Camellia sinensis / Q000737:chemistry*; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D019610:Cytoprotection / Q000187:drug effects; D005285:Fermentation; D056945:Hep G2 Cells; D006801:Humans; D052197:Limosilactobacillus fermentum / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000378:metabolism* / Q000494:pharmacology*; D019936:Probiotics / Q000494:pharmacology
chemical_list: D000438:Alcohols; D010936:Plant Extracts; D000426:Alcohol Dehydrogenase; D000444:Aldehyde Dehydrogenase