title: Rapid quantitative CBF and CMRO(2) measurements from a single PET scan with sequential administration of dual (15)O-labeled tracers.
abstract: Positron emission tomography (PET) with (15)O tracers provides essential information in patients with cerebral vascular disorders, such as cerebral blood flow (CBF), oxygen extraction fraction (OEF), and metabolic rate of oxygen (CMRO(2)). However, most of techniques require an additional C(15)O scan for compensating cerebral blood volume (CBV). We aimed to establish a technique to calculate all functional images only from a single dynamic PET scan, without losing accuracy or statistical certainties. The technique was an extension of previous dual-tracer autoradiography (DARG) approach, but based on the basis function method (DBFM), thus estimating all functional parametric images from a single session of dynamic scan acquired during the sequential administration of H(2)(15)O and (15)O(2). Validity was tested on six monkeys by comparing global OEF by PET with those by arteriovenous blood sampling, and tested feasibility on young healthy subjects. The mean DBFM-derived global OEF was 0.57±0.06 in monkeys, in an agreement with that by the arteriovenous method (0.54±0.06). Image quality was similar and no significant differences were seen from DARG; 3.57%±6.44% and 3.84%±3.42% for CBF, and -2.79%±11.2% and -6.68%±10.5% for CMRO(2). A simulation study demonstrated similar error propagation between DBFM and DARG. The DBFM method enables accurate assessment of CBF and CMRO(2) without additional CBV scan within significantly shortened examination period, in clinical settings.
authors.affiliation: Department of Investigative Radiology, National Cerebral and Cardiovascular Center, Research Institute, Suita, Osaka, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kudomi
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Hirano
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Koshino
authors.forename: Takuya
authors.initials: T
authors.lastname: Hayashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Watabe
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Fukushima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Moriwaki
authors.forename: Noboru
authors.initials: N
authors.lastname: Teramoto
authors.forename: Koji
authors.initials: K
authors.lastname: Iihara
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Iida
mesh_terms: D000818:Animals; D005260:Female; D008252:Macaca fascicularis; D018810:Magnetic Resonance Angiography / Q000379:methods*; D008297:Male; D008955:Models, Cardiovascular; D010100:Oxygen / Q000378:metabolism*; D010103:Oxygen Isotopes / Q000494:pharmacology; D049268:Positron-Emission Tomography / Q000379:methods*
chemical_list: D010103:Oxygen Isotopes; D010100:Oxygen