title: Inhibition of binding of Vibrio vulnificus hemolysin (VVH) by MβCD.
abstract: Vibrio vulnificus secrets a pore-forming toxin called Vibrio vulnificus hemolysin (VVH). In this study, we showed that methyl-beta-cyclodextrin (MβCD), an oligosaccharide, decreased binding of VVH to Chinese hamster ovary (CHO) cells, resulting in inhibition of its cytotoxicity. When the VVH was incubated with MβCD, cytotoxicity of the toxin was inhibited from 100.3 ± 7.2% to 19.6 ± 5.3%. Binding analysis showed that the amount of VVH on the cells was decreased from 101.4 ± 9.2% to 18.1 ± 8.0% only when MβCD was present in the culture media. Our results indicate that the inhibition of cytotoxicity of VVH by MβCD was due to a decrease in the amount of toxin binding to CHO cells.
authors.affiliation: Laboratory of Veterinary Public Health, School of Veterinary Medicine, Kitasato University, 23-35-1 Higashi, Towada, Aomori 034-8628, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sugiyama
authors.forename: Takashige
authors.initials: T
authors.lastname: Kashimoto
authors.forename: Shunji
authors.initials: S
authors.lastname: Ueno
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Susa
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism* / Q000633:toxicity*; D016466:CHO Cells; D018938:Caco-2 Cells; D006224:Cricetinae; D003412:Cricetulus; D006460:Hemolysin Proteins / Q000378:metabolism* / Q000633:toxicity*; D006801:Humans; D011485:Protein Binding; D041261:Vibrio vulnificus / Q000378:metabolism*; D047392:beta-Cyclodextrins / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D006460:Hemolysin Proteins; D047392:beta-Cyclodextrins; C073845:hemolysin, Vibrio; C108732:methyl-beta-cyclodextrin