title: Effect of quercetin-5'-sulfonic acid sodium salt on SOD activity and ADMA/DDAH pathway in extracorporeal liver perfusion in rats.
abstract: BACKGROUND
  Quercetin-5'-sulfonic acid sodium salt (NaQSA) exerts good aqueous solubility, strong antioxidant activity and low toxicity.
  
  
  OBJECTIVE
  The aims of this study were to investigate the effect of NaQSA on superoxide dismutase (SOD) activity and ADMA/DDAH pathway during extracorporeal liver perfusion (ELP).
  
  
  METHODS
  The study was carried out on male Wistar rats. Isolated livers were perfused with Krebs-Henseleit bicarbonate buffer (KHB) + 1 microM ADMA (group C), or with KHB + 1 microM ADMA and either 10 microM NaQSA (Q10) or 50 microM NaQSA (Q50). In group 0 (sham) livers were perfused with KHB alone. Levels of ADMA, alanine (ALT) and aspartate (AST) aminotransferases activities were measured during perfusion. After 90 min. of perfusion superoxide dismutase (SOD) and dimethylarginine dimethylaminohydrolase (DDAH) activities were estimated in liver homogenates.
  
  
  RESULTS
  DDAH activity in Q10 group was significantly higher as compared to control and Q50 groups. No significant differences were observed between Q50 and control group. The decrease in ADMA concentration during perfusion was observed in all groups, but the most pronounced in the group Q10 and the least in group Q50. During perfusion AST activities were the lowest in Q50 group. No significant difference in SOD activity in groups perfused with NaQSA as compared to control group was noted.
  
  
  CONCLUSIONS
  The impact of NaQSA on ADMA/DDAH system depends on its concentration. In lower concentration NaQSA exerted some beneficial properties which vanished in higher concentration. No increase in SOD activity during perfusion with NaQSA was observed.
authors.affiliation: Department of Pharmacology, Wroclaw Medical University, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Trocha
authors.forename: Anna
authors.initials: A
authors.lastname: Merwid-Lad
authors.forename: Andrzej
authors.initials: A
authors.lastname: Szuba
authors.forename: Tomasz
authors.initials: T
authors.lastname: Sozański
authors.forename: Jan
authors.initials: J
authors.lastname: Magdalan
authors.forename: Adam
authors.initials: A
authors.lastname: Szelag
authors.forename: Maria
authors.initials: M
authors.lastname: Kopacz
authors.forename: Anna
authors.initials: A
authors.lastname: Kuźniar
authors.forename: Dorota
authors.initials: D
authors.lastname: Nowak
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000581:Amidohydrolases / Q000378:metabolism*; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D010477:Perfusion; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: C502510:quercetin-5'-sulfonic acid; C018524:N,N-dimethylarginine; D001120:Arginine; D011794:Quercetin; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000581:Amidohydrolases; C059999:dimethylargininase