title: Inflammatory serum proteome pattern in mice fed a high-fat diet.
abstract: To investigate the influence of diet on serum protein pattern, mice were fed for 8 weeks either control chow or a high-fat diet (containing 21 % w/w milk fat and 0.2 % w/w cholesterol); sera were collected and analyzed by 2-DE. The main positive acute-phase reactant proteins, haptoglobin and hemopexin, were significantly up-regulated in animals receiving the high-fat diet. Data on all other proteins also pointed to an inflammatory condition in these animals. The largest change in concentration was observed for carboxylesterase N, a circulating enzyme seldom connected with lipid metabolism in earlier reports. These observations agree with the notion of a link between diet-induced hyperlipidemia and the inflammatory component of its cardiovascular sequels in humans, but the effects in the experimental animals are massive and obviously affect most of the major serum proteins.
authors.affiliation: Gruppo di Studio per la Proteomica e la Struttura delle Proteine, Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, via G. Balzaretti 9, 20133 Milan, Italy. elisabetta.gianazza@unimi.it
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Gianazza
authors.forename: Cristina
authors.initials: C
authors.lastname: Sensi
authors.forename: Ivano
authors.initials: I
authors.lastname: Eberini
authors.forename: Federica
authors.initials: F
authors.lastname: Gilardi
authors.forename: Marco
authors.initials: M
authors.lastname: Giudici
authors.forename: Maurizio
authors.initials: M
authors.lastname: Crestani
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007249:Inflammation / Q000209:etiology / Q000235:genetics / Q000378:metabolism*; D050356:Lipid Metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020543:Proteome / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D044967:Serum / Q000737:chemistry / Q000378:metabolism
chemical_list: D001798:Blood Proteins; D020543:Proteome