title: [Study on the HPLC-DAD fingerprint chromatograms of Radix Rubiae from different areas in China].
abstract: OBJECTIVE
  To establish the HPLC-DAD fingerprint of Radix Rubiae and provide a quality control method for it.
  
  
  METHODS
  The method HPLC-DAD was performed on a YMC C,18 (250 mm x 4.6 mm, 5 microm) column with gradient elution of acetonitrile-0.2% phosphoric acid solution as mobile phase, flow rate at 1.0 mL/min, detection wavelength at 280 mm, and the column temperature at 25 degrees C, and the analysis time was 60 min.
  
  
  RESULTS
  The average similarity of the fnigerprint chromatograms was over 0.94 in 6 batches samples of different areas in China.
  
  
  CONCLUSIONS
  This method can be applied for the determination of Radix Rubiae from different areas.
authors.affiliation: Wuhan Botanic Garden, Chinese Academy of Sciences, Wuhan 430074, China. yuanxiaomail@yahoo.com.cn
authors.forename: Xiao
authors.initials: X
authors.lastname: Yuan
authors.forename: Jun-fei
authors.initials: JF
authors.lastname: Gao
authors.forename: Ping
authors.initials: P
authors.lastname: Yuan
mesh_terms: D000880:Anthraquinones / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry / Q000254:growth & development; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development; D011786:Quality Control; D015203:Reproducibility of Results; D045470:Rubia / Q000737:chemistry* / Q000254:growth & development
chemical_list: D000880:Anthraquinones; D004365:Drugs, Chinese Herbal