title: Identification of the promoter region and genetic mutations of the porcine GALP gene.
abstract: Galanin-like peptide (GALP) gene, encoding a member of the galanin family of neuropeptides involved in reproduction, was differentially expressed in PMSG-hCG stimulated pre-ovulatory ovarian follicles of Chinese Taihu and Large White sows in our previous study. In the present study, promoter region and genetic mutations of the porcine GALP gene were determined. A 1,322 bp contig in 5'-flanking region was predicted to contain 5 potential transcription promoters by Neural Network Promoter Prediction version 2.2. 5'-deletion expression in both CHO and hela cells showed that there were a negative regulatory element at -852 to -803 bp and a positive regulatory element at -1,318 to -1,269 bp. Comparative sequence analyses of Chinese Taihu and Large White GALP gene sequence revealed the c.*27C>G mutation in the 3'-UTR and the c.88-1225C>G mutation in intron 1, which can be detected by HhaI and AluI PCR-RFLP, respectively. The association analysis with litter size traits showed that at both loci CC and GG genotypes were different for NBA for all parities in DIV pigs (P < 0.05). However, two SNPs were not in significant linkage disequilibrium analyzed using SHEsis online software, and could be used in pig breeding individually.
authors.affiliation: Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Lina
authors.initials: L
authors.lastname: Su
authors.forename: Shuqi
authors.initials: S
authors.lastname: Mei
authors.forename: Hu
authors.initials: H
authors.lastname: Tao
authors.forename: Xianwen
authors.initials: X
authors.lastname: Peng
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Sun
authors.forename: Huayu
authors.initials: H
authors.lastname: Wu
authors.forename: Xuying
authors.initials: X
authors.lastname: Zhang
authors.forename: Mu
authors.initials: M
authors.lastname: Qiao
authors.forename: Fenge
authors.initials: F
authors.lastname: Li
mesh_terms: D000818:Animals; D044089:Galanin-Like Peptide / Q000235:genetics*; D056726:Genetic Association Studies; D008098:Litter Size / Q000235:genetics*; D009154:Mutation; D011401:Promoter Regions, Genetic; D040641:Quantitative Trait Loci; D013552:Swine / Q000235:genetics*
chemical_list: D044089:Galanin-Like Peptide