title: Human haemato-endothelial precursors: cord blood CD34+ cells produce haemogenic endothelium.
abstract: Embryologic and genetic evidence suggest a common origin of haematopoietic and endothelial lineages. In the murine embryo, recent studies indicate the presence of haemogenic endothelium and of a common haemato-endothelial precursor, the haemangioblast. Conversely, so far, little evidence supports the presence of haemogenic endothelium and haemangioblasts in later stages of development. Our studies indicate that human cord blood haematopoietic progenitors (CD34+45+144-), triggered by murine hepatocyte conditioned medium, differentiate into adherent proliferating endothelial precursors (CD144+CD105+CD146+CD31+CD45-) capable of functioning as haemogenic endothelium. These cells, proven to give rise to functional vasculature in vivo, if further instructed by haematopoietic growth factors, first switch to transitional CD144+45+ cells and then to haematopoietic cells. These results highlight the plasticity of haemato-endhothelial precursors in human post-natal life. Furthermore, these studies may provide highly enriched populations of human post-fetal haemogenic endothelium, paving the way for innovative projects at a basic and possibly clinical level.
authors.affiliation: Department of Haematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy. elvira.pelosi@iss.it
authors.forename: Elvira
authors.initials: E
authors.lastname: Pelosi
authors.forename: Germana
authors.initials: G
authors.lastname: Castelli
authors.forename: Ines
authors.initials: I
authors.lastname: Martin-Padura
authors.forename: Veronica
authors.initials: V
authors.lastname: Bordoni
authors.forename: Simona
authors.initials: S
authors.lastname: Santoro
authors.forename: Alice
authors.initials: A
authors.lastname: Conigliaro
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Cerio
authors.forename: Marco
authors.initials: M
authors.lastname: De Santis Puzzonia
authors.forename: Paola
authors.initials: P
authors.lastname: Marighetti
authors.forename: Mauro
authors.initials: M
authors.lastname: Biffoni
authors.forename: Tonino
authors.initials: T
authors.lastname: Alonzi
authors.forename: Laura
authors.initials: L
authors.lastname: Amicone
authors.forename: Myriam
authors.initials: M
authors.lastname: Alcalay
authors.forename: Francesco
authors.initials: F
authors.lastname: Bertolini
authors.forename: Ugo
authors.initials: U
authors.lastname: Testa
authors.forename: Marco
authors.initials: M
authors.lastname: Tripodi
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism*; D015820:Cadherins / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005312:Fetal Blood / Q000166:cytology*; D055018:Hemangioblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006410:Hematopoiesis / Q000187:drug effects; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects; D006801:Humans; D016130:Immunophenotyping; D007231:Infant, Newborn; D017493:Leukocyte Common Antigens / Q000378:metabolism; D051379:Mice; D010641:Phenotype; D000099152:Cadherin 5
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D015820:Cadherins; D017077:Culture Media, Conditioned; D017493:Leukocyte Common Antigens; D000099152:Cadherin 5