title: Rapid hepatitis C virus divergence among chronically infected individuals.
abstract: Here, we analyze the viral divergence among hepatitis C virus (HCV) chronic cases infected with genotype 1. The intrahost viral evolution was assessed by deep sequencing using the 454 Genome Sequencer platform. The results showed a rapid nucleotide sequence divergence. This notorious short-term viral evolution is of the utmost importance for the study of HCV transmission, because direct links between related samples were virtually lost. Thus, rapid divergence of HCV significantly affects genetic relatedness studies and outbreak investigations.
authors.affiliation: Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico.
authors.forename: Mayra
authors.initials: M
authors.lastname: Cruz-Rivera
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Carpio-Pedroza
authors.forename: Alejandro
authors.initials: A
authors.lastname: Escobar-Gutiérrez
authors.forename: Daniela
authors.initials: D
authors.lastname: Lozano
authors.forename: Arely
authors.initials: A
authors.lastname: Vergara-Castaneda
authors.forename: Pilar
authors.initials: P
authors.lastname: Rivera-Osorio
authors.forename: Armando
authors.initials: A
authors.lastname: Martinez-Guarneros
authors.forename: Carlos A Vazquez
authors.initials: CA
authors.lastname: Chacon
authors.forename: Salvador
authors.initials: S
authors.lastname: Fonseca-Coronado
authors.forename: Gilberto
authors.initials: G
authors.lastname: Vaughan
mesh_terms: D000328:Adult; D000368:Aged; D019143:Evolution, Molecular; D005260:Female; D014644:Genetic Variation; D016679:Genome, Viral; D005838:Genotype; D016174:Hepacivirus / Q000145:classification / Q000235:genetics*; D019698:Hepatitis C, Chronic / Q000235:genetics / Q000821:virology*; D006801:Humans; D007372:Interferons; D007378:Interleukins / Q000235:genetics; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D012307:Risk Factors; D017422:Sequence Analysis, DNA; D000095022:Interferon Lambda
chemical_list: D007372:Interferons; D007378:Interleukins; C000624353:interferon-lambda, human; D000095022:Interferon Lambda