title: Modulation of PAR expression and tryptic enzyme induced IL-4 production in mast cells by IL-29.
abstract: Interleukin (IL)-29 is a relatively newly discovered cytokine, which has been shown to be actively involved in the pathogenesis of allergic inflammation. However, little is known of the effects of IL-29 on protease activated receptor (PAR) expression and potential mechanisms of cytokine production in mast cells. In the present study, we examined potential influence of IL-29 on PAR expression and cytokine production in P815 and bone marrow derived mast cells (BMMCs) by using flow cytometry analysis, quantitative real time PCR, and ELISA techniques. The results showed that IL-29 downregulated the expression of PAR-1 by up to 56.2%, but had little influence on the expression of PAR-2, PAR-3 and PAR-4. IL-29 also induced downregulation of expression of PAR-1 mRNA. However, when mast cells were pre-incubated with IL-29, thrombin-, trypsin- and tryptase-induced expression of PAR-2, PAR-3 and PAR-4 was upregulated, respectively. IL-29 provoked approximately up to 1.9-fold increase in IL-4 release when mast cells was challenged with IL-29. Administration of IL-29 blocking antibody, AG490 or LY294002 abolished IL-29-induced IL-4 release from P815 cells. It was found that IL-29 diminished trypsin- and tryptase-induced IL-4 release from P815 cells following 16 h incubation. In conclusion, IL-29 can regulate expression of PARs and tryptase- and trypsin-induced IL-4 production in mast cells, through which participates in the mast cell related inflammation.
authors.affiliation: Department of Pathophysiology, Hainan Medical College, Haikou, Hainan 571101, China.
authors.forename: Huiyun
authors.initials: H
authors.lastname: Zhang
authors.forename: Haiwei
authors.initials: H
authors.lastname: Yang
authors.forename: Wenjing
authors.initials: W
authors.lastname: Ma
authors.forename: Zhongfang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Shaoheng
authors.initials: S
authors.lastname: He
mesh_terms: D000818:Animals; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007372:Interferons; D015847:Interleukin-4 / Q000096:biosynthesis* / Q000378:metabolism; D007378:Interleukins / Q000494:pharmacology*; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D044462:Receptors, Proteinase-Activated / Q000235:genetics / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism*; D053802:Tryptases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000095022:Interferon Lambda
chemical_list: D007372:Interferons; D015847:Interleukin-4; D007378:Interleukins; D012333:RNA, Messenger; D044462:Receptors, Proteinase-Activated; D014357:Trypsin; D053802:Tryptases; C000624353:interferon-lambda, human; D000095022:Interferon Lambda