title: Specific IgA and metalloproteinase activity in bronchial secretions from stable chronic obstructive pulmonary disease patients colonized by Haemophilus influenzae.
abstract: BACKGROUND
  Haemophilus influenzae is the most common colonizing bacteria of the bronchial tree in chronic obstructive pulmonary disease (COPD), and positive cultures for this potentially pathogenic microorganism (PPM) has been associated with local inflammation changes that may influence the relationships between H. influenzae and the bronchial mucosa.
  
  
  METHODS
  A cross-sectional analysis of stable COPD patients enrolled in the Phenotype and Course of Chronic Obstructive Pulmonary Disease (PAC-COPD) Study, focusing on bronchial colonization by H. influenzae, was performed. Specific IgA against the PPM was measured by optical density, and metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) using ELISA in sputum samples. Levels in patients colonized by H. influenzae and non-colonized patients were compared.
  
  
  RESULTS
  Sputum supernatant for the measurement of specific IgA against H. influenzae was available from 54 stable COPD patients, who showed levels of specific IgA significantly lower in colonized (n=21) than in non-colonized patients (n=33) (15 [4-37] versus 31 [10-75], p=0.033, Mann-Whitney U test). Proenzyme MMP-9 was measured in 44 patients, and it was higher in colonized (n=12, 1903 [1488-6699] ng/ml) than in non-colonized patients (n=32, 639 [373-972] ng/ml) (p<0.001, Mann-Whitney U test). Active form of MMP-9 was also higher in colonized (126 [25-277] ng/ml) than in non-colonized patients (39 [14-68] ng/ml) (p=0.021, Mann-Whitney U test), and the molar ratio between proenzyme MMP-9 and TIMP-1 was above 1 (2.1 [0.1-12.5]) in colonized patients, significantly higher than the ratio found in non-colonized patients (0.2 [0.08-0.5]) (p=0.030, Mann-Whitney U test).
  
  
  CONCLUSIONS
  Clinically stable COPD patients colonized by H. influenzae had lower levels of specific IgA against the microorganism and higher values of the active form of MMP-9 in their sputum supernatant than non-colonized patients. Bronchial colonization by H. influenzae may cause structural changes in the extracellular matrix through a defective defense and the production of active metalloproteinases.
authors.affiliation: Fundació Parc Taulí, Sabadell/Badalona, Spain. lmillares@tauli.cat
authors.forename: Laura
authors.initials: L
authors.lastname: Millares
authors.forename: Alicia
authors.initials: A
authors.lastname: Marin
authors.forename: Judith
authors.initials: J
authors.lastname: Garcia-Aymerich
authors.forename: Jaume
authors.initials: J
authors.lastname: Sauleda
authors.forename: José
authors.initials: J
authors.lastname: Belda
authors.forename: Eduard
authors.initials: E
authors.lastname: Monsó
mesh_terms: D000368:Aged; D001980:Bronchi / Q000378:metabolism* / Q000382:microbiology*; D005260:Female; D006193:Haemophilus influenzae / Q000302:isolation & purification*; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism*; D008297:Male; D045726:Metalloproteases / Q000378:metabolism*; D029424:Pulmonary Disease, Chronic Obstructive / Q000378:metabolism* / Q000382:microbiology*; D020545:Respiratory Mucosa / Q000378:metabolism / Q000382:microbiology; D013183:Sputum / Q000378:metabolism / Q000382:microbiology
chemical_list: D007070:Immunoglobulin A; D045726:Metalloproteases