title: The protective role of yeast cathepsin D in acetic acid-induced apoptosis depends on ANT (Aac2p) but not on the voltage-dependent channel (Por1p).
abstract: We have previously shown that the yeast Cathepsin D (CatD) Pep4p translocates from the vacuole to the cytosol during acetic acid-induced apoptosis and is required for efficient mitochondrial degradation, though its specific role in this process is still elusive. Here, we show that the protective role of Pep4p in acetic acid-induced apoptosis depends on its catalytic activity and is independent of the yeast voltage-dependent anion channel Por1p (which has no role on mitochondrial degradation) but dependent on AAC proteins, the yeast adenine nucleotide translocator. Our results demonstrate a differential interplay between yeast vacuolar CatD and mitochondrial proteins involved in apoptosis regulation.
authors.affiliation: Centro de Biologia Molecular e Ambiental, Departamento de Biologia, Universidade do Minho, Braga, Portugal.
authors.forename: Helena
authors.initials: H
authors.lastname: Pereira
authors.forename: Flávio
authors.initials: F
authors.lastname: Azevedo
authors.forename: António
authors.initials: A
authors.lastname: Rego
authors.forename: Maria João
authors.initials: MJ
authors.lastname: Sousa
authors.forename: Susana R
authors.initials: SR
authors.lastname: Chaves
authors.forename: Manuela
authors.initials: M
authors.lastname: Côrte-Real
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology; D016282:Aspartic Acid Endopeptidases / Q000235:genetics / Q000378:metabolism; D001693:Biological Transport, Active; D002402:Cathepsin D / Q000378:metabolism*; D005800:Genes, Fungal; D008928:Mitochondria / Q000378:metabolism; D000226:Mitochondrial ADP, ATP Translocases / Q000378:metabolism*; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism; D050994:Voltage-Dependent Anion Channels / Q000378:metabolism*
chemical_list: C112225:PET9 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D050994:Voltage-Dependent Anion Channels; D000226:Mitochondrial ADP, ATP Translocases; C499267:PEP4 protein, S cerevisiae; D016282:Aspartic Acid Endopeptidases; D002402:Cathepsin D; D019342:Acetic Acid