title: Artificial repressors for controlling gene expression in bacteria.
abstract: Transcriptional repression is a common approach to control gene expression in synthetic biology applications. Here, an engineered DNA binding protein based upon a transcription activator-like effector (TALE) scaffold was shown to outperform LacI in blocking transcription from a promoter and to repress expression of a downstream gene in an operon.
authors.affiliation: University of Wisconsin-Madison, Department of Chemical and Biological Engineering, 3629 Engineering Hall, 1415 Engineering Dr Madison, WI, 53706, USA.
authors.forename: Mark C
authors.initials: MC
authors.lastname: Politz
authors.forename: Matthew F
authors.initials: MF
authors.lastname: Copeland
authors.forename: Brian F
authors.initials: BF
authors.lastname: Pfleger
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D056968:Lac Repressors / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014974:Xanthomonas / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D056968:Lac Repressors