title: Optimizing the enzyme loading and incubation time in enzymatic hydrolysis of lignocellulosic substrates.
abstract: A mathematical model for costing enzymatic hydrolysis of lignocellulosics is presented. This model is based on three variable parameters describing substrate characteristics and three unit costs for substrate, enzymes and incubation. The model is used to minimize the cost of fermentable sugars, as intermediate products on the route to ethanol or other biorefinery products, by calculating optimized values of enzyme loading and incubation time. This approach allows comparisons between substrates, with processing conditions optimized independently for each substrate. Steam-exploded pine wood was hydrolyzed in order to test the theoretical relationship between sugar yield and processing conditions.
authors.affiliation: Scion, Private Bag 3020, Rotorua Mail Centre, Rotorua 3046, New Zealand. Roger.Newman@scionresearch.com
authors.forename: Roger H
authors.initials: RH
authors.lastname: Newman
authors.forename: Alankar A
authors.initials: AA
authors.lastname: Vaidya
authors.forename: M Imroz
authors.initials: MI
authors.lastname: Sohel
authors.forename: Michael W
authors.initials: MW
authors.lastname: Jack
mesh_terms: D002480:Cellulase / Q000737:chemistry* / Q000191:economics*; D003198:Computer Simulation; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry* / Q000191:economics*; D018803:Models, Economic; D009520:New Zealand; D014934:Wood / Q000737:chemistry* / Q000191:economics*
chemical_list: C036909:lignocellulose; D008031:Lignin; D002480:Cellulase