title: Polymorphisms in intron 1 of the EGFR gene in non-small cell lung cancer patients.
abstract: The epidermal growth factor receptor (EGFR) gene is highly polymorphic and its expression and activity may be affected by various polymorphisms. There have been several studies examining associations between EGFR polymorphisms and clinical outcome of lung cancer therapy; however, the underlying mechanism is largely unknown. The present study investigated EGFR polymorphism status and its correlation with clinicopathological features in Japanese non-small cell lung cancer (NSCLC) patients. We investigated 5 polymorphisms in the EGFR gene (-216G/T, -191C/A, 8227G/A, D994D and R497K) in 274 surgically-treated NSCLC patients. TaqMan single nucleotide polymorphism (SNP) genotyping assays and a PCR-based assay were used to analyze these polymorphisms. In our cohort of patients we did not find any evidence of the -191C/A polymorphism. Our results showed that the patients with the 8227GA or AA type in intron 1 had a significantly better prognosis with the anti-EGFR therapy than the patients with the GG type (p=0.0448) in terms of recurrence of lung cancer. No significant association was observed between 3 other SNPs (-216G/T, D994D and R497K) and clinicopathological features. The EGFR 8227G/A polymorphism in intron 1 may be associated with clinical outcome in NSCLC patients treated with EGFR tyrosine kinase inhibitors.
authors.affiliation: Department of Oncology, Immunology and Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya 467-8601;
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shitara
authors.forename: Hidefumi
authors.initials: H
authors.lastname: Sasaki
authors.forename: Keisuke
authors.initials: K
authors.lastname: Yokota
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Okuda
authors.forename: Yu
authors.initials: Y
authors.lastname: Hikosaka
authors.forename: Satoru
authors.initials: S
authors.lastname: Moriyama
authors.forename: Motoki
authors.initials: M
authors.lastname: Yano
authors.forename: Tomoya
authors.initials: T
authors.lastname: Kawaguchi
authors.forename: Akihito
authors.initials: A
authors.lastname: Kubo
authors.forename: Minoru
authors.initials: M
authors.lastname: Takada
authors.forename: Naoto
authors.initials: N
authors.lastname: Kitahara
authors.forename: Meinoshin
authors.initials: M
authors.lastname: Okumura
authors.forename: Akihide
authors.initials: A
authors.lastname: Matsumura
authors.forename: Keiji
authors.initials: K
authors.lastname: Iuchi
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Fujii