title: Sulfation of keratan sulfate proteoglycan reduces radiation-induced apoptosis in human Burkitt's lymphoma cell lines.
abstract: This study focuses on clarifying the contribution of sulfation to radiation-induced apoptosis in human Burkitt's lymphoma cell lines, using 3'-phosphoadenosine 5'-phosphosulfate transporters (PAPSTs). Overexpression of PAPST1 or PAPST2 reduced radiation-induced apoptosis in Namalwa cells, whereas the repression of PAPST1 expression enhanced apoptosis. Inhibition of PAPST slightly decreased keratan sulfate (KS) expression, so that depletion of KS significantly increased radiation-induced apoptosis. In addition, the repression of all three N-acetylglucosamine-6-O-sulfotransferases (CHST2, CHST6, and CHST7) increased apoptosis. In contrast, PAPST1 expression promoted the phosphorylation of p38 MAPK and Akt in irradiated Namalwa cells. These findings suggest that 6-O-sulfation of GlcNAc residues in KS reduces radiation-induced apoptosis of human Burkitt's lymphoma cells.
authors.affiliation: Advanced Radiation Biology Research Program, Research Center for Charged Particle Therapy, National Institute of Radiological Sciences, Chiba, Japan. f_naka@nirs.go.jp
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Nakayama
authors.forename: Sachiko
authors.initials: S
authors.lastname: Umeda
authors.forename: Tomomi
authors.initials: T
authors.lastname: Ichimiya
authors.forename: Shin
authors.initials: S
authors.lastname: Kamiyama
authors.forename: Masaharu
authors.initials: M
authors.lastname: Hazawa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yasuda
authors.forename: Shoko
authors.initials: S
authors.lastname: Nishihara
authors.forename: Takashi
authors.initials: T
authors.lastname: Imai
mesh_terms: D027321:Anion Transport Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D017209:Apoptosis / Q000502:physiology / Q000528:radiation effects; D001483:Base Sequence; D002051:Burkitt Lymphoma / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy; D045744:Cell Line, Tumor; D006801:Humans; D007632:Keratan Sulfate / Q000737:chemistry / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D011509:Proteoglycans / Q000737:chemistry / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D011836:Radiation Tolerance / Q000502:physiology; D000076988:Sulfate Transporters; D013463:Sulfuric Acid Esters / Q000378:metabolism
chemical_list: D027321:Anion Transport Proteins; D026901:Membrane Transport Proteins; D011509:Proteoglycans; D034741:RNA, Small Interfering; C476248:SLC35B2 protein, human; C510864:SLC35B3 protein, human; D000076988:Sulfate Transporters; D013463:Sulfuric Acid Esters; D007632:Keratan Sulfate