title: Cloning, heterologous expression, and enzymatic characterization of cathepsin L from starfish (Asterina pectinifera).
abstract: To determine the role of cathepsin L in Echinoderms, starfish (Asterina pectinifera) cathepsin L (ApCtL) was cloned. The results of RT-PCR analysis indicated that the expression of ApCtL in all of the tissues. The pro-mature enzyme of ApCtL, proApCtL, was expressed in Escherichia coli, and cathepsin activity was detected by cleaving of synthetic fluorogenic peptide substrates and gelatin zymography.
authors.affiliation: Department of Biotechnology, Pukyong National University, Busan, Korea.
authors.forename: A Ram
authors.initials: AR
authors.lastname: Lee
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Bak
authors.forename: Na Young
authors.initials: NY
authors.lastname: Kim
authors.forename: Moo-Sang
authors.initials: MS
authors.lastname: Kim
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Go
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Han
authors.forename: Sang Jung
authors.initials: SJ
authors.lastname: Ahn
authors.forename: Nam Gyu
authors.initials: NG
authors.lastname: Park
authors.forename: Joon Ki
authors.initials: JK
authors.lastname: Chung
authors.forename: Hyung Ho
authors.initials: HH
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D047288:Asterina / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D056668:Cathepsin L / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data
chemical_list: D056668:Cathepsin L