title: Replication study of 15 recently published Loci for body fat distribution in the Japanese population.
abstract: OBJECTIVE
  Visceral fat accumulation plays an integral role in morbidity and mortality rates by increasing the risk of developing metabolic disorders such as type 2 diabetes, dyslipidemia, and hypertension. New genetic loci associated with fat distribution, measured by waist-hip ratios and computed tomography (CT), have recently been identified by genome-wide association studies in European-descent populations. This study used CT to investigate whether single nucleotide polymorphisms (SNPs) that confer susceptibility to fat distribution are associated with visceral fat area (VFA) and subcutaneous fat area (SFA) in the Japanese population.
  
  
  METHODS
  We measured the VFAs and SFAs of 1424 obese Japanese subjects (BMI≥25 kg/m(2), 635 men and 789 women) that were genotyped at 15 SNPs, namely, TBX15 rs984222, DNM3 rs1011731, LYPLAL1 rs4846567, GRB14 rs10195252, NISCH rs6784615, ADAMTS9 rs6795735, CPEB4 rs6861681, LY86 rs1294421, VEGFA rs6905288, RSPO3 rs9491696, NFE2L3 rs1055144, ITPR2 rs718314, HOXC13 rs1443512, ZNRF3 rs4823006 and THNSL2 rs1659258.
  
  
  RESULTS
  The G-allele of LYPLAL1 rs4846567 was borderline associated with an increased ratio of VFA to SFA (V/S ratio; p= 0.0020). LYPLAL1 rs4846567 had a stronger effect on the V/S ratio in women (p= 0.0078) than in men (p= 0.12); however, neither result was significant after Bonferroni correction for multiple comparisons. NISCH rs6784615 was nominally associated with increased VFA (p=0.040) and V/S ratio (p= 0.020). The other SNPs analyzed were not significantly associated with body mass index (BMI), VFA, or SFA.
  
  
  CONCLUSIONS
  Our results suggest that LYPLAL1 rs4846567 and NISCH rs6784615 may influence fat distribution in the Japanese population.
authors.affiliation: EBM Research Center, Kyoto University Graduate School of Medicine, Kyoto, Japan. kikukoh@kuhp.kyoto-u.ac.jp
authors.forename: Kikuko
authors.initials: K
authors.lastname: Hotta
authors.forename: Aya
authors.initials: A
authors.lastname: Kitamoto
authors.forename: Takuya
authors.initials: T
authors.lastname: Kitamoto
authors.forename: Seiho
authors.initials: S
authors.lastname: Mizusawa
authors.forename: Hajime
authors.initials: H
authors.lastname: Teranishi
authors.forename: Rina
authors.initials: R
authors.lastname: So
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Matsuo
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Nakata
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Hyogo
authors.forename: Hidenori
authors.initials: H
authors.lastname: Ochi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nakamura
authors.forename: Seika
authors.initials: S
authors.lastname: Kamohara
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Miyatake
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Kotani
authors.forename: Naoto
authors.initials: N
authors.lastname: Itoh
authors.forename: Ikuo
authors.initials: I
authors.lastname: Mineo
authors.forename: Jun
authors.initials: J
authors.lastname: Wada
authors.forename: Masato
authors.initials: M
authors.lastname: Yoneda
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nakajima
authors.forename: Tohru
authors.initials: T
authors.lastname: Funahashi
authors.forename: Shigeru
authors.initials: S
authors.lastname: Miyazaki
authors.forename: Katsuto
authors.initials: K
authors.lastname: Tokunaga
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Masuzaki
authors.forename: Takato
authors.initials: T
authors.lastname: Ueno
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Chayama
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Hamaguchi
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yamada
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Hanafusa
authors.forename: Shinichi
authors.initials: S
authors.lastname: Oikawa
authors.forename: Toshiie
authors.initials: T
authors.lastname: Sakata
authors.forename: Kiyoji
authors.initials: K
authors.lastname: Tanaka
authors.forename: Yuji
authors.initials: Y
authors.lastname: Matsuzawa
authors.forename: Kazuwa
authors.initials: K
authors.lastname: Nakao
authors.forename: Akihiro
authors.initials: A
authors.lastname: Sekine
mesh_terms: D044466:Asian People / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics; D050171:Dyslipidemias / Q000235:genetics; D005260:Female; D056426:Genetic Loci; D006801:Humans; D006973:Hypertension / Q000235:genetics; D050152:Intra-Abdominal Fat; D007564:Japan; D008297:Male; D008659:Metabolic Diseases / Q000235:genetics; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D014057:Tomography, X-Ray Computed; D049629:Waist-Hip Ratio