title: [Association between mannose-binding lectin 2 gene and protein kinase C-beta 1 gene polymorphisms and type 2 diabetic macrovascular complications in northern Chinese Han population].
abstract: OBJECTIVE
  To assess the association between single nucleotide polymorphisms (SNPs) of mannose-binding lectin 2 gene (MBL2) (rs1800450, rs1800451 and rs11003125) and protein kinase C-beta 1 gene (PRKC beta 1) (rs3700106, rs2575390) with diabetic macroangiopathy in northern Chinese Han population.
  
  
  METHODS
  The samples have included 318 type 2 diabetes mellitus (T2DM) patients and 448 normoglycemic controls. The five SNPs were determined by a Multiplex SnaPshot method. Biochemical indices such as fasting plasma-glucose, triglyceride and total cholesterol were also measured. Linkage disequilibrium and haplotype analysis were carried out for all samples using Haploview 4.2. Additive model was applied to assess the effect of interaction between SNPs and environment factors on macrovascular complications.
  
  
  RESULTS
  Genotypic frequencies of rs11003125 have differed significantly between the controls and patients with coronary heart disease and peripheral vascular disease (P=0.024 and 0.004, respectively). The allele frequency of rs11003125 was also statistically significant between the two groups (P=0.014 and 0.001, respectively). Compared with patients without macrovascular complications, the allele frequency of rs11003125 was significantly different in patients with peripheral vascular disease (P=0.031). No significant differences were found between the distribution of the genotype frequency and allele frequencies of other variants. Haplotype analysis indicated that, compared with controls and patients without macrovascular complications, individuals with G allele of rs1800450 and C allele of rs11003125 had a higher risk for macrovascular complications.
  
  
  CONCLUSIONS
  The rs11003125 polymorphism located in the promoter region of MBL2 gene is associated with macrovascular complications of T2DM in northern Chinese Han population. G allele of rs1800450 and C allele of rs11003125 may be risk factors for macrovascular complications. There were additive interactive effects for rs11003125 polymorphism (GC+CC) and hypertension, diabetic nephropathy, diabetic neuropathy and diabetic retinopathy on macrovascular complications.
authors.affiliation: Clinical Epidemiology Unit, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P R China.
authors.forename: Na-na
authors.initials: NN
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Yao
authors.forename: Mao-qiang
authors.initials: MQ
authors.lastname: Zhuang
authors.forename: Guo-chang
authors.initials: GC
authors.lastname: Wang
authors.forename: Tian-tian
authors.initials: TT
authors.lastname: Chen
authors.forename: Ya-jun
authors.initials: YJ
authors.lastname: Yang
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Lv
authors.forename: Li
authors.initials: L
authors.lastname: Jin
mesh_terms: D000483:Alleles; D002681:China / Q000208:ethnology; D003924:Diabetes Mellitus, Type 2 / Q000208:ethnology / Q000235:genetics*; D003925:Diabetic Angiopathies / Q000208:ethnology / Q000235:genetics*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D037601:Mannose-Binding Lectin / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D011493:Protein Kinase C / Q000235:genetics*; D064546:Protein Kinase C beta
chemical_list: C445619:MBL2 protein, human; D037601:Mannose-Binding Lectin; D011493:Protein Kinase C; D064546:Protein Kinase C beta