title: Sequential triplex real-time PCR assay for detecting 21 pneumococcal capsular serotypes that account for a high global disease burden.
abstract: We developed and validated a real-time PCR assay consisting of 7 triplexed reactions to identify 11 individual serotypes plus 10 small serogroups representing the majority of disease-causing isolates of Streptococcus pneumoniae. This assay targets the 13 serotypes included within the 13-valent conjugate vaccine and 8 additional key serotypes or serogroups. Advantages over other serotyping assays are described. The assay will be expanded to 40 serotypes/serogroups. We will provide periodic updates at our protocol website.
authors.affiliation: Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Fabiana C
authors.initials: FC
authors.lastname: Pimenta
authors.forename: Alexis
authors.initials: A
authors.lastname: Roundtree
authors.forename: Ahmet
authors.initials: A
authors.lastname: Soysal
authors.forename: Mustafa
authors.initials: M
authors.lastname: Bakir
authors.forename: Mignon
authors.initials: M
authors.lastname: du Plessis
authors.forename: Nicole
authors.initials: N
authors.lastname: Wolter
authors.forename: Anne
authors.initials: A
authors.lastname: von Gottberg
authors.forename: Lesley
authors.initials: L
authors.lastname: McGee
authors.forename: Maria da Gloria
authors.initials: Mda G
authors.lastname: Carvalho
authors.forename: Bernard
authors.initials: B
authors.lastname: Beall
mesh_terms: D005798:Genes, Bacterial; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction; D011008:Pneumococcal Infections / Q000453:epidemiology / Q000517:prevention & control; D022242:Pneumococcal Vaccines; D060888:Real-Time Polymerase Chain Reaction; D012703:Serotyping; D013291:Streptococcus / Q000145:classification* / Q000235:genetics*; D018074:Vaccines, Conjugate
chemical_list: D022242:Pneumococcal Vaccines; D018074:Vaccines, Conjugate