title: Fluorescent probe for highly selective and sensitive detection of hydrogen sulfide in living cells and cardiac tissues.
abstract: A coumarin-based fluorescence chemoprobe was developed and evaluated for the selective and sensitive detection of hydrogen sulfide in degassed PBS buffers and fetal bovine serum. Fluorescence detection of hydrogen sulfide in living cells was also successfully achieved using two-photon confocal fluorescence imaging. Further in situ visualization of endogenous H(2)S was realized in cardiac tissues of normal rats and atherosclerosis (AS) rats.
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Rd, Beijing 100191, China.
authors.forename: Bifeng
authors.initials: B
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Cong
authors.initials: C
authors.lastname: Lv
authors.forename: Manman
authors.initials: M
authors.lastname: Zhao
authors.forename: Hongwei
authors.initials: H
authors.lastname: Jin
authors.forename: Hongfang
authors.initials: H
authors.lastname: Jin
authors.forename: Junbao
authors.initials: J
authors.lastname: Du
authors.forename: Liangren
authors.initials: L
authors.lastname: Zhang
authors.forename: Xinjing
authors.initials: X
authors.lastname: Tang
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000378:metabolism / Q000473:pathology; D002417:Cattle; D003374:Coumarins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D018613:Microscopy, Confocal; D009206:Myocardium / Q000737:chemistry*; D051381:Rats
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes; D006862:Hydrogen Sulfide