title: Thermostable Bacteriocin BL8 from Bacillus licheniformis isolated from marine sediment.
abstract: OBJECTIVE
  To isolate and characterize bacteriocin, BL8, from the bacteria identified as Bacillus licheniformis from marine environment.
  
  
  RESULTS
  One-hundred and twelve bacterial isolates from sediment and water samples collected off the coast of Cochin, India, were screened for antibacterial activity. Strain BTHT8, identified as Bacillus licheniformis, inhibited the growth of Gram-positive test organisms. The active component labelled as bacteriocin BL8 was partially purified by ammonium sulphate fractionation and was subjected to glycine SDS-PAGE. The band exhibiting antimicrobial activity was electroeluted and analysed using MALDI-TOF mass spectrometry, and the molecular mass was determined as 1.4 kDa. N-terminal amino acid sequencing of BL8 gave a 13 amino acid sequence stretch. Bacteriocin BL8 was stable even after boiling at 100 °C for 30 min and over a wide pH range of 1-12.
  
  
  CONCLUSIONS
  A novel, pH-tolerant and thermostable bacteriocin BL8, active against the tested Gram-positive bacteria, was isolated from Bacillus licheniformis.
  
  
  CONCLUSIONS
  This study reports a stable, low molecular weight bacteriocin from Bacillus licheniformis. This bacteriocin can be used to address two important applications: as a therapeutic agent and as a biopreservative in food processing industry.
authors.affiliation: Department of Biotechnology, Cochin University of Science and Technology, Cochin, India.
authors.forename: S
authors.initials: S
authors.lastname: Smitha
authors.forename: S G
authors.initials: SG
authors.lastname: Bhat
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001407:Bacillus / Q000737:chemistry* / Q000302:isolation & purification; D001430:Bacteriocins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D019015:Geologic Sediments / Q000382:microbiology*; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006358:Hot Temperature; D007194:India; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D055550:Protein Stability; D020539:Sequence Analysis, Protein
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins