title: Whole-genome detection of disease-associated deletions or excess homozygosity in a case-control study of rheumatoid arthritis.
abstract: Unlike genome-wide association studies, few comprehensive studies of copy number variation's contribution to complex human disease susceptibility have been performed. Copy number variations are abundant in humans and represent one of the least well-studied classes of genetic variants; in addition, known rheumatoid arthritis susceptibility loci explain only a portion of familial clustering. Therefore, we performed a genome-wide study of association between deletion or excess homozygosity and rheumatoid arthritis using high-density 550 K SNP genotype data from a genome-wide association study. We used a genome-wide statistical method that we recently developed to test each contiguous SNP locus between 868 cases and 1194 controls to detect excess homozygosity or deletion variants that influence susceptibility. Our method is designed to detect statistically significant evidence of deletions or homozygosity at individual SNPs for SNP-by-SNP analyses and to combine the information among neighboring SNPs for cluster analyses. In addition to successfully detecting the known deletion variants on major histocompatibility complex, we identified 4.3 and 28 kb clusters on chromosomes 10p and 13q, respectively, which were significant at a Bonferroni-type-corrected 0.05 nominal significant level. Independently, we performed analyses using PennCNV, an algorithm for identifying and cataloging copy numbers for individuals based on a hidden Markov model, and identified cases and controls that had chromosomal segments with copy number <2. Using Fisher's exact test for comparing the numbers of cases and controls with copy number <2 per SNP, we identified 26 significant SNPs (protective; more controls than cases) aggregating on chromosome 14 with P-values <10(-8).
authors.affiliation: Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. ccwu@mdanderson.org
authors.forename: Chih-Chieh
authors.initials: CC
authors.lastname: Wu
authors.forename: Sanjay
authors.initials: S
authors.lastname: Shete
authors.forename: Eun-Ji
authors.initials: EJ
authors.lastname: Jo
authors.forename: Yaji
authors.initials: Y
authors.lastname: Xu
authors.forename: Emily Y
authors.initials: EY
authors.lastname: Lu
authors.forename: Wei V
authors.initials: WV
authors.lastname: Chen
authors.forename: Christopher I
authors.initials: CI
authors.lastname: Amos
mesh_terms: D001172:Arthritis, Rheumatoid / Q000235:genetics*; D016022:Case-Control Studies; D056915:DNA Copy Number Variations; D005260:Female; D055106:Genome-Wide Association Study; D006720:Homozygote; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide; D017384:Sequence Deletion