title: Analysis of corky off-flavour compounds at ultra trace level with multidimensional gas chromatography-electron capture detection.
abstract: A robust method for routine quality control of corky off-flavour compounds in wine and cork soak matrices has been established. Based on an automated headspace solid phase microextraction (HS-SPME), the method needs only marginal sample preparation and achieves low (sub-ng L(-1)) trace level detection limits (LODs) for the most relevant off-flavour compounds, such as 2,4,6-trichloroanisole (TCA), 2,3,4,6-tetrachloroanisole (TeCA) and 2,4,6-tribromoanisole (TBA). Particularly for wine matrix, reliable trace level quantification had only been achieved after applying heart-cutting multidimensional gas chromatography (MDGC). Using a halogen-sensitive electron capture detector (ECD) and quantification with a stable isotope dilution assay (SIDA), LODs of 0.1ng L(-1) for TCA, TeCA and TBA could be obtained. Since a SIDA based quantification method is used with a non-mass spectrometric detector, the necessary chromatographic resolution of internal standard and target analyte peaks resulted from the use of highly deuterated [(2)H(5)]-isotopologues.
authors.affiliation: Dienstleistungszentrum Ländlicher Raum (DLR) Rheinpfalz, Competence Centre for Wine Research, Breitenweg 71, D-67435 Neustadt an der Weinstrasse, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Slabizki
authors.forename: Hans-Georg
authors.initials: HG
authors.lastname: Schmarr
mesh_terms: D000840:Anisoles / Q000032:analysis*; D002138:Calibration; D002849:Chromatography, Gas / Q000379:methods*; D057230:Limit of Detection; D009812:Odorants / Q000032:analysis*; D052617:Solid Phase Microextraction; D014920:Wine / Q000032:analysis*
chemical_list: D000840:Anisoles