title: Effects of Fufang Shenhua Tablet on the expression of toll-like receptors during acute kidney injury induced by ischemia-reperfusion in rats.
abstract: OBJECTIVE
  To investigate the impact of a traditional Chinese medicinal compound known as Fufang Shenhua Tablet (SHP) on the expression of Toll-like receptors (TLRs) during renal ischemia-reperfusion injury (IRI)-induced acute kidney injury (AKI) in rats.
  
  
  METHODS
  A total of 28 Wistar rats were randomly divided into five groups: (1) pseudo-operation control group, (2) ischemia-reperfusion model group, (3) Astragaloside group, (4) high-dose SHP group, and (5) low-dose SHP group. There were four rats in the pseudo-operation group and six rats in each of the other groups. The accepted ischemia-reperfusion model was established after a 7-day gavage intervention, and pathological changes and renal function were observed, using an enzyme-linked immunosorbent assay (ELISA) to detect interleukin 8 (IL-8) and interferon gamma (IFN-γ) levels, as well as immunohistochemical staining to detect altered levels of TLR2 and TLR4 expression in renal tissue.
  
  
  RESULTS
  After 24 h, renal pathological damage and the expression levels of serum creatinine (Scr), IL-8, IFN-γ, TLR2, and TLR4 were significantly higher in the model group as compared with the pseudo-operation group (P<0.05). In addition, at 24 h the above indicators decreased significantly in the Astragaloside group, high-dose SHP group and low-dose SHP group as compared with the ischemia-reperfusion model group (P< 0.05). TLR2 and TLR4 expression levels were significantly reduced in the SHP treatment and Astragaloside group as compared with the pseudo-operation group (P<0.05). Further, the high-dose SHP group showed significantly less renal damage score and decreased levels of TLR expression than those of low-dose SHP group and Astragaloside group (all P<0.05).
  
  
  CONCLUSIONS
  SHP can alleviate the renal structural and functional damage caused by IRI-induced AKI in rats by reducing the damage of renal pathology, which may reduce inflammatory cytokine levels by downregulating the expression of TLRs in renal tissue in a dose-dependent manner.
authors.affiliation: Nephropathy of Chinese PLA General Hospital, State Discipline and Key Laboratory of Kidney Disease-Chinese PLA General Hospital 2011DAV00088, Beijing 100853, China.
authors.forename: Xiao-yong
authors.initials: XY
authors.lastname: Zheng
authors.forename: Ri-bao
authors.initials: RB
authors.lastname: Wei
authors.forename: Suo-zhu
authors.initials: SZ
authors.lastname: Shi
authors.forename: Zhong
authors.initials: Z
authors.lastname: Yin
authors.forename: Xiang-mei
authors.initials: XM
authors.lastname: Chen
mesh_terms: D058186:Acute Kidney Injury / Q000209:etiology / Q000378:metabolism*; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D007684:Kidney Tubules / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications*; D013607:Tablets; D051195:Toll-Like Receptor 2 / Q000378:metabolism*; D051197:Toll-Like Receptor 4 / Q000378:metabolism*
chemical_list: D004365:Drugs, Chinese Herbal; D016209:Interleukin-8; D013607:Tablets; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; C581148:fufang shenhua; D007371:Interferon-gamma