title: Optical detection of downy mildew in grapevine leaves: daily kinetics of autofluorescence upon infection.
abstract: A 15-day survey of autofluorescence has been conducted upon infection by downy mildew [Plasmopara viticola (Berk. & M.A. Curtis) Berl. & de Toni] of leaves of a susceptible grapevine genotype. Different autofluorescence signals were followed from the cellular to the whole-leaf level by using four types of devices for fluorosensing: a macroscope, a spectrofluorimeter, a portable field optical sensor (the Multiplex 3), and a field fluorescence sensor prototype with 335 nm excitation. It was shown for the first time, by the three different techniques and at three different scales, that the stilbene-dependent violet-blue autofluorescence (VBF) had a transitory behaviour, increasing to a maximum 6 days post-inoculation (DPI) and then decreasing to a constant lower level, nevertheless significantly higher than in the control leaf. This behaviour could be sensed from both sides of the leaf. On the abaxial side, VBF could discriminate the presence of infection from 1 DPI, and on the adaxial side from 3 DPI. There was a constant increase in blue-excited green fluorescence starting from 8 DPI, concomitant with a decrease in leaf chlorophyll content sensed by one reflectance and two fluorescence indices available on the Multiplex 3 sensor. These results show that a pre-symptomatic and symptomatic sensing of downy mildew is possible by autofluorescence-based sensors, and this is potentially applicable in the field.
authors.affiliation: Université Paris-Sud, Laboratoire Écologie Systématique et Évolution, UMR 8079, Bât. 362, 91405 Orsay; CNRS, 91405 Orsay; AgroParisTech, 75231 Paris,  France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Bellow
authors.forename: Gwendal
authors.initials: G
authors.lastname: Latouche
authors.forename: Spencer C
authors.initials: SC
authors.lastname: Brown
authors.forename: Anne
authors.initials: A
authors.lastname: Poutaraud
authors.forename: Zoran G
authors.initials: ZG
authors.lastname: Cerovic
mesh_terms: D007700:Kinetics; D061848:Optical Imaging / Q000379:methods*; D044742:Peronospora / Q000502:physiology* / Q000528:radiation effects; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology* / Q000528:radiation effects; D013050:Spectrometry, Fluorescence; D014466:Ultraviolet Rays; D027843:Vitis / Q000382:microbiology* / Q000528:radiation effects