title: [Establishment of drug screening assay and pharmacodynamic evaluation method targeting influenza RNA polymerase].
abstract: Influenza virus RNA-dependent RNA polymerase (RdRP) is essential for replication and expression of influenza virus genome. Viral genomic sequences encoding RdRP are highly conservative, thus making it a potential anti-influenza drug target. A cell-based influenza RdRP inhibitor screening assay was established by a luciferase reporter system to analyze the activity of RdRP. Specificity study and statistic analysis showed that the screening assay is sensitive and reproducible.
authors.affiliation: Department of lmmunology, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Zhen
authors.initials: Z
authors.lastname: He
authors.forename: Zhen-Long
authors.initials: ZL
authors.lastname: Liu
authors.forename: Xiao-Lu
authors.initials: XL
authors.lastname: Wei
authors.forename: Xiao
authors.initials: X
authors.lastname: Yin
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Zhou
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Li
authors.forename: Zhi-Zhen
authors.initials: ZZ
authors.lastname: Zhang
authors.forename: Shan
authors.initials: S
authors.lastname: Cen
mesh_terms: D000547:Amantadine / Q000494:pharmacology; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D017930:Genes, Reporter; D057809:HEK293 Cells; D006801:Humans; D029521:Alphainfluenzavirus / Q000201:enzymology; D008156:Luciferases / Q000235:genetics / Q000378:metabolism*; D053139:Oseltamivir / Q000494:pharmacology; D010957:Plasmids; D012324:RNA-Dependent RNA Polymerase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D015203:Reproducibility of Results; D012254:Ribavirin / Q000494:pharmacology; D012680:Sensitivity and Specificity; D014162:Transfection; D053243:Zanamivir / Q000494:pharmacology
chemical_list: D000998:Antiviral Agents; D053139:Oseltamivir; D012254:Ribavirin; D000547:Amantadine; D008156:Luciferases; D012324:RNA-Dependent RNA Polymerase; D053243:Zanamivir