title: Nandrolone decanoate induces genetic damage in multiple organs of rats.
abstract: To evaluate the impact potential of nandrolone decanoate on DNA damage in multiple organs of Wistar rats by means of single-cell gel (comet) assay and micronucleus test. A total of 15 animals were distributed into three groups of five animals each as follows: control group = animal not exposed to nandrolone decanoate; experimental group = animals exposed to nandrolone decanoate for 24 h at 5 mg/kg subcutaneously; and experimental group = animals exposed to nandrolone decanoate for 24 h at 15 mg/kg subcutaneously. Significant statistical differences (p < 0.05) were noted in peripheral blood, liver, and heart cells exposed to nandrolone decanoate at the two doses evaluated. A clear dose-response relationship was observed between groups. Kidney cells showed genetic damage at only the highest dose (15 mg/kg) used. However, micronucleus data did not show remarkable differences among groups. In conclusion, the present study indicates that nandrolone decanoate induces genetic damage in rat blood, liver, heart, and kidney cells as shown by single-cell gel (comet) assay results.
authors.affiliation: Department of Biosciences, Federal University of São Paulo, São Paulo, 11060-001, Brazil.
authors.forename: Renan
authors.initials: R
authors.lastname: Pozzi
authors.forename: Kelly Rosseti
authors.initials: KR
authors.lastname: Fernandes
authors.forename: Carolina Foot Gomes
authors.initials: CF
authors.lastname: de Moura
authors.forename: Raquel Agnelli Mesquita
authors.initials: RA
authors.lastname: Ferrari
authors.forename: Kristianne Porta Santos
authors.initials: KP
authors.lastname: Fernandes
authors.forename: Ana Claudia Muniz
authors.initials: AC
authors.lastname: Renno
authors.forename: Daniel Araki
authors.initials: DA
authors.lastname: Ribeiro
mesh_terms: D045930:Anabolic Agents / Q000633:toxicity*; D000818:Animals; D020552:Comet Assay; D004249:DNA Damage; D003627:Data Interpretation, Statistical; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects / Q000473:pathology; D006321:Heart / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced*; D015162:Micronucleus Tests; D009277:Nandrolone / Q000633:toxicity*; D009928:Organ Specificity; D051381:Rats; D017208:Rats, Wistar; D018675:Toxicity Tests
chemical_list: D045930:Anabolic Agents; D009277:Nandrolone