title: Ex vivo study of bacterial coronal leakage in indirect pulp treatment.
abstract: OBJECTIVE
  The aim of this study was to evaluate, ex vivo, bacterial coronal leakage with different antimicrobial agents applied to the dentine for indirect pulp treatment (IPT).
  
  
  METHODS
  Sixty extracted teeth were prepared and randomly distributed into 5 groups (n=10): Group 1: no antimicrobial dentine treatment; group 2: 1% chlorhexidine (CHX)+1% thymol varnish (Cervitec); group 3: 2 % CHX solution; group 4: 40% CHX varnish (EC40) and group 5: Clearfil Protect Bond (CPB). Ten teeth served as controls. The teeth were restored using a resin-modified glass ionomer cement (GIC) and then mounted in a two-chamber device. The coronal access was exposed to Streptococcus mutans for 45 days. The appearance of turbidity in the BHI broth of the lower chamber was considered as specimen leakage.
  
  
  RESULTS
  Survival analysis, determined by non parametric Kaplan-Meier and log-rank tests, showed that the best results were for groups EC40+GIC and GIC alone; yet there were not statistically significant differences between them. All specimens of CPB+GIC and 2% CHX+GIC, leaked at 45 days.
  
  
  CONCLUSIONS
  In IPT the use of GIC without pretreatment of the dentine and pretreatment with 40% CHX varnish resulted in a significant delay of bacterial coronal leakage.
authors.affiliation: Department of Paediatric Dentistry, School of Dentistry, University of Granada, Campus de Cartuja, Colegio Máximo s/n, Granada, Spain. matr@ugr.es
authors.forename: Matilde
authors.initials: M
authors.lastname: Ruiz
authors.forename: Pilar
authors.initials: P
authors.lastname: Baca
authors.forename: Maria-del Mar
authors.initials: MD
authors.lastname: Pardo-Ridao
authors.forename: Maria-Teresa
authors.initials: MT
authors.lastname: Arias-Moliz
authors.forename: Carmen-María
authors.initials: CM
authors.lastname: Ferrer-Luque
mesh_terms: D000890:Anti-Infective Agents / Q000627:therapeutic use*; D003763:Dental Leakage / Q000382:microbiology* / Q000517:prevention & control*; D003782:Dental Pulp; D003804:Dentin; D006801:Humans; D066298:In Vitro Techniques; D013295:Streptococcus mutans
chemical_list: D000890:Anti-Infective Agents