title: Light harvesting and blue-green light induced non-photochemical quenching in two different C-phycocyanin mutants of Synechocystis PCC 6803.
abstract: Cyanobacteria are oxygen-evolving photosynthetic organisms that harvest sunlight and convert excitation energy into chemical energy. Most of the light is absorbed by large light harvesting complexes called phycobilisomes (PBs). In high-light conditions, cyanobacteria switch on a photoprotective mechanism called non-photochemical quenching (NPQ): During this process, absorption of blue-green light transforms the inactive orange form of the orange carotenoid protein OCP (OCP(o)) into the red active form OCP(r) that subsequently binds to the PB, resulting in a substantial loss of excitation energy and corresponding decrease of the fluorescence. In wild-type cells, the quenching site is a bilin chomophore that fluoresces at 660 nm and which is called APC(Q)(660). In the present work, we studied NPQ in two different types of mutant cells (CB and CK) that possess significantly truncated PBs, using spectrally resolved picosecond fluorescence spectroscopy. The results are in very good agreement with earlier in vitro experiments on quenched and unquenched PBs, although the fraction of quenched PBs is far lower in vivo. It is also lower than the fraction of PBs that is quenched in wild-type cells, but the site, rate, and location of quenching appear to be very similar.
authors.affiliation: Laboratory of Biophysics, Wageningen University , P.O. Box 8128, 6700 ET, Wageningen, The Netherlands.
authors.forename: Lijin
authors.initials: L
authors.lastname: Tian
authors.forename: Ivo H M
authors.initials: IH
authors.lastname: van Stokkum
authors.forename: Rob B M
authors.initials: RB
authors.lastname: Koehorst
authors.forename: Herbert
authors.initials: H
authors.lastname: van Amerongen
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008027:Light; D009154:Mutation; D010798:Phycocyanin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D046939:Synechocystis / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D010798:Phycocyanin