title: [Method establishment for the determination of 8 organophosphorus pesticides in serum using LC/MS/MS].
abstract: OBJECTIVE
  To establish the method to determine the presence and concentration of 8 organophosphors pesticides in serum by LC/MS/MS.
  
  
  METHODS
  The serum samples were extracted by ethyl acetateether and then separated on C18 5 microm (2.1 mm x 150 mm) column with pH = 3.5 acid-ammonium acetate buffer solution, methanol--water as mobile phase to mixed gradient elution. Electro-spray ionization source (ESI) was applied and operated in positive ion mode, and multiple reaction monitoring(MRM) mode was adopted.
  
  
  RESULTS
  Within concentration ranging from 0-1000 ng/ml the correlation coefficient for each compound was greater than 0.990, recoveries were between 95%-129%, RSD < 7.9%. The RSD of intra-day and inter-day were less 12% (n = 6).
  
  
  CONCLUSIONS
  The procedure of preparing serum samples is sample and the result of this method is efficient, accurate and specific.
authors.affiliation: Institute for Environmental Health and Related Product Safety, Chinese CDC, Beijing 100021, China.
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Zhou
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Shaobin
authors.initials: S
authors.lastname: Lin
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D009943:Organophosphorus Compounds / Q000097:blood*; D010573:Pesticide Residues / Q000097:blood*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009943:Organophosphorus Compounds; D010573:Pesticide Residues