title: Impact of diet on development of bronchial-associated immunity in the neonatal piglet.
abstract: Bronchial-associated immune development is critically important to protect neonates from respiratory infections. Herein, bronchial-associated immune development in formula-fed and sow-reared pigs is described. Colostrum-fed newborn piglets were fed medicated sow milk replacer formula beginning at 48 h of life or remained with the sow. Blood and tissues were sampled at one-week (d7) and three-weeks (d21) of age. Lymphocyte subpopulations, including T helper 2, cytotoxic T, memory T, and NK cells, in peripheral blood, mediastinal lymph nodes, and thoracic lymph nodes were identified using flow cytometry. Additionally, IL-1β, IL-6, IL-12, TNFα, TGF-β1, TGF-β2, IFNα, IFNβ, and dectin gene expression were analyzed by quantitative RT-PCR. Total IgG, IgM, and IgA concentrations in serum were analyzed. Dietary and developmental effects were observed. This set of baseline measurements provides a framework for future respiratory challenge studies where the effects of diet on the neonate's ability to resist and/or recover from infection can be tested.
authors.affiliation: Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USA.
authors.forename: Shannon C
authors.initials: SC
authors.lastname: Thorum
authors.forename: Sarah S
authors.initials: SS
authors.lastname: Comstock
authors.forename: Shelly N
authors.initials: SN
authors.lastname: Hester
authors.forename: Jill M
authors.initials: JM
authors.lastname: Shunk
authors.forename: Marcia H
authors.initials: MH
authors.lastname: Monaco
authors.forename: Sharon M
authors.initials: SM
authors.lastname: Donovan
mesh_terms: D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D001980:Bronchi / Q000276:immunology*; D016207:Cytokines / Q000235:genetics; D004032:Diet; D005260:Female; D015870:Gene Expression; D007136:Immunoglobulins / Q000097:blood; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D016131:Lymphocyte Subsets / Q000276:immunology; D008892:Milk; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D034421:Sus scrofa / Q000097:blood / Q000235:genetics / Q000276:immunology*
chemical_list: D016207:Cytokines; D007136:Immunoglobulins; D012333:RNA, Messenger