title: Cryo-electron tomography of bacterial viruses.
abstract: Bacteriophage particles contain both simple and complex macromolecular assemblages and machines that enable them to regulate the infection process under diverse environmental conditions with a broad range of bacterial hosts. Recent developments in cryo-electron tomography (cryo-ET) make it possible to observe the interactions of bacteriophages with their host cells under native-state conditions at unprecedented resolution and in three-dimensions. This review describes the application of cryo-ET to studies of bacteriophage attachment, genome ejection, assembly and egress. Current topics of investigation and future directions in the field are also discussed.
authors.affiliation: Division of Pediatric Infectious Diseases, Emory University School of Medicine, Children's Healthcare of Atlanta, Atlanta, GA 30322, USA.
authors.forename: Ricardo C
authors.initials: RC
authors.lastname: Guerrero-Ferreira
authors.forename: Elizabeth R
authors.initials: ER
authors.lastname: Wright
mesh_terms: D001419:Bacteria / Q000821:virology; D001435:Bacteriophages / Q000235:genetics* / Q000648:ultrastructure*; D020285:Cryoelectron Microscopy; D004279:DNA, Viral; D055032:Electron Microscope Tomography / Q000379:methods; D016679:Genome, Viral; D021621:Imaging, Three-Dimensional; D014771:Virion / Q000235:genetics / Q000648:ultrastructure*
chemical_list: D004279:DNA, Viral