title: Jacalin regulates IgA production by peripheral blood mononuclear cells.
abstract: OBJECTIVE
  In IgA nephropathy, circulating immune complexes containing IgA1 are deposited on the glomerular mesangium, causing mesangial cell proliferation and acceleration of extracellular matrix production. The suppressive effect of jacalin, a galactose-binding lectin, on IgA production in vitro was determined.
  
  
  METHODS
  Normal human peripheral blood mononuclear cells were stimulated with plate-bound anti-CD3 and Th2 stimulation, with or without jacalin. Regulatory and effector cell subsets were determined by flow cytometry, and immunoglobulin production by ELISA.
  
  
  RESULTS
  Jacalin increased the ratio of CD4(+)CD25(+)CD152(+) Tregs:effector T cells in peripheral blood mononuclear cell cultures 60-fold. This CD4(+)CD25(+)CD152(+) Treg increase may have inhibited Th2-stimulated IgA production by B cells.
  
  
  CONCLUSIONS
  Immune tolerance induced by jacalin can suppress IgA production.
authors.affiliation: Department of Chemistry for Materials, Graduate School of Engineering, Mie University, 1577 Kurima-machiya-cho, Tsu, Mie 514-8507, Japan. miyamoto@chem.mie-u.ac.jp
authors.forename: Keiichi
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Takeshi
authors.initials: T
authors.lastname: Chiba
authors.forename: Noriko
authors.initials: N
authors.lastname: Shinohara
authors.forename: Yuko
authors.initials: Y
authors.lastname: Nagata
authors.forename: Nagisa
authors.initials: N
authors.lastname: Asakawa
authors.forename: Shinji
authors.initials: S
authors.lastname: Kato
authors.forename: Naoki
authors.initials: N
authors.lastname: Mizutani
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Murata
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Nomura
authors.forename: Takashi
authors.initials: T
authors.lastname: Horiuchi
mesh_terms: D000917:Antibody Formation / Q000187:drug effects; D015703:Antigens, CD / Q000276:immunology; D002469:Cell Separation; D002478:Cells, Cultured; D005434:Flow Cytometry; D005922:Glomerulonephritis, IGA / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000096:biosynthesis*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D037121:Plant Lectins / Q000494:pharmacology*; D016176:T-Lymphocyte Subsets / Q000187:drug effects* / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology; D018418:Th2 Cells / Q000187:drug effects* / Q000276:immunology
chemical_list: D015703:Antigens, CD; D007070:Immunoglobulin A; D007166:Immunosuppressive Agents; D037121:Plant Lectins; C043696:jacalin