title: Phospholipase C gamma-1 is required for granulocyte maturation in zebrafish.
abstract: The regulation of hematopoiesis is generally evolutionarily conserved from zebrafish to mammals, including hematopoietic stem cell formation and blood cell lineage differentiation. In zebrafish, primitive granulocytes originate at two distinct regions, the anterior lateral plate mesoderm (A-LPM) and the intermediate cell mass (ICM). Few studies in the zebrafish have examined genes specifically required for the granulocytic lineage. In this study, we identified the responsible gene for a zebrafish mutant that has relatively normal hematopoiesis, except decreased expression of the granulocyte-specific gene mpx. Positional cloning revealed that phospholipase C gamma-1 (plcg1) was mutated. Deficiency of plcg1 function specifically affected development of granulocytes, especially the maturation process. These results suggested that plcg1 functioned specifically in zebrafish ICM granulopoiesis for the first time. Our studies suggest that specific pathways regulate the differentiation of the hematopoietic lineages.
authors.affiliation: Key Laboratory of Stem Cell Biology, Laboratory of Development and Diseases, Institute of Health Sciences, Shanghai Jiao Tong University School of Medicine & Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, People's Republic of China.
authors.forename: Chang-Bin
authors.initials: CB
authors.lastname: Jing
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.forename: Mei
authors.initials: M
authors.lastname: Dong
authors.forename: Xiao-Lan
authors.initials: XL
authors.lastname: Peng
authors.forename: Xiao-E
authors.initials: XE
authors.lastname: Jia
authors.forename: Lei
authors.initials: L
authors.lastname: Gao
authors.forename: Ke
authors.initials: K
authors.lastname: Ma
authors.forename: Min
authors.initials: M
authors.lastname: Deng
authors.forename: Ting-Xi
authors.initials: TX
authors.lastname: Liu
authors.forename: Leonard I
authors.initials: LI
authors.lastname: Zon
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D018507:Gene Expression Regulation, Developmental; D006098:Granulocytes / Q000166:cytology*; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006657:Histones / Q000378:metabolism; D018613:Microscopy, Confocal / Q000379:methods; D008957:Models, Genetic; D016296:Mutagenesis; D009154:Mutation; D010641:Phenotype; D051966:Phospholipase C gamma / Q000235:genetics / Q000502:physiology*; D010766:Phosphorylation; D010957:Plasmids / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D015027:Zebrafish
chemical_list: D006657:Histones; D012333:RNA, Messenger; D051966:Phospholipase C gamma