title: Occupationally acquired american cutaneous leishmaniasis.
abstract: WE REPORT TWO OCCUPATIONALLY ACQUIRED CASES OF AMERICAN CUTANEOUS LEISHMANIASIS (ACL): one accidental laboratory autoinoculation by contaminated needlestick while handling an ACL lesion sample, and one acquired during field studies on bird biology. Polymerase chain reaction (PCR) assays of patient lesions were positive for Leishmania, subgenus Viannia. One isolate was obtained by culture (from patient 2 biopsy samples) and characterized as Leishmania (Viannia) naiffi through an indirect immunofluorescence assay (IFA) with species-specific monoclonal antibodies (mAbs) and by multilocus enzyme electrophoresis (MLEE). Patients were successfully treated with N-methyl-glucamine. These two cases highlight the potential risks of laboratory and field work and the need to comply with strict biosafety procedures in daily routines. The swab collection method, coupled with PCR detection, has greatly improved ACL laboratory diagnosis.
authors.affiliation: Centro de Pesquisas Aggeu Magalhães, FIOCRUZ-PE, Campus da Universidade Federal de Pernambuco s/n, Cidade Universitária, 50.670-901 Recife, PE, Brazil ; Centro de Ciências Biológicas, Universidade Federal de Pernambuco s/n, Cidade Universitária, 50.670-901 Recife, PE, Brazil.
authors.forename: Maria Edileuza
authors.initials: ME
authors.lastname: Felinto de Brito
authors.forename: Maria Sandra
authors.initials: MS
authors.lastname: Andrade
authors.forename: Ericka Lima
authors.initials: EL
authors.lastname: de Almeida
authors.forename: Angela Cristina Rapela
authors.initials: AC
authors.lastname: Medeiros
authors.forename: Roberto Pereira
authors.initials: RP
authors.lastname: Werkhäuser
authors.forename: Ana Isabele Freitas
authors.initials: AI
authors.lastname: de Araújo
authors.forename: Sinval Pinto
authors.initials: SP
authors.lastname: Brandão-Filho
authors.forename: Alzira Maria
authors.initials: AM
authors.lastname: Paiva de Almeida
authors.forename: Eduardo Henrique
authors.initials: EH
authors.lastname: Gomes Rodrigues