title: MicroRNA-30c targets cytoskeleton genes involved in breast cancer cell invasion.
abstract: Metastasis remains a significant challenge in treating cancer. A better understanding of the molecular mechanisms underlying metastasis is needed to develop more effective treatments. Here, we show that human breast tumor biomarker miR-30c regulates invasion by targeting the cytoskeleton network genes encoding twinfilin 1 (TWF1) and vimentin (VIM). Both VIM and TWF1 have been shown to regulate epithelial-to-mesenchymal transition. Similar to TWF1, VIM also regulates F-actin formation, a key component of cellular transition to a more invasive mesenchymal phenotype. To further characterize the role of the TWF1 pathway in breast cancer, we found that IL-11 is an important target of TWF1 that regulates breast cancer cell invasion and STAT3 phosphorylation. The miR-30c-VIM/TWF1 signaling cascade is also associated with clinical outcome in breast cancer patients.
authors.affiliation: The Ben May Department for Cancer Research, The University of Chicago, Chicago, IL 60637, USA.
authors.forename: Jessica
authors.initials: J
authors.lastname: Bockhorn
authors.forename: Kathy
authors.initials: K
authors.lastname: Yee
authors.forename: Ya-Fang
authors.initials: YF
authors.lastname: Chang
authors.forename: Aleix
authors.initials: A
authors.lastname: Prat
authors.forename: Dezheng
authors.initials: D
authors.lastname: Huo
authors.forename: Chika
authors.initials: C
authors.lastname: Nwachukwu
authors.forename: Rachel
authors.initials: R
authors.lastname: Dalton
authors.forename: Simo
authors.initials: S
authors.lastname: Huang
authors.forename: Kaitlin E
authors.initials: KE
authors.lastname: Swanson
authors.forename: Charles M
authors.initials: CM
authors.lastname: Perou
authors.forename: Olufunmilayo I
authors.initials: OI
authors.lastname: Olopade
authors.forename: Michael F
authors.initials: MF
authors.lastname: Clarke
authors.forename: Geoffrey L
authors.initials: GL
authors.lastname: Greene
authors.forename: Huiping
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D003599:Cytoskeleton / Q000235:genetics* / Q000473:pathology; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017370:Interleukin-11 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011505:Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism; D014746:Vimentin / Q000235:genetics* / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
chemical_list: C508611:IL11 protein, human; D017370:Interleukin-11; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; D008840:Microfilament Proteins; C485188:TWF1 protein, human; D014746:Vimentin; D011505:Protein-Tyrosine Kinases