title: Direct comparison of the potency of human mesenchymal stem cells derived             from amnion tissue, bone marrow and adipose tissue at inducing dermal fibroblast             responses to cutaneous wounds.
abstract: Although transplantation of human mesenchymal stem cells (MSCs) derived from amnion (hAMSCs), bone marrow (hBMSCs) and adipose tissues (hADSCs) has been shown to aid in the repair of cutaneous wounds in mouse models, little information is available regarding the relative efficacy of MSCs from different sources. In this study, we compared their therapeutic potentials by transplanting equal numbers of hAMSCs, hBMSCs or hADSCs in a mouse model of cutaneous wounds. The results suggested that an hADSC injection has the most pronounced effect on wound closure. Histological evaluation showed enhanced re-epithelialization in the hADSC group compared with the hBMSC and hAMSC groups. Although there was a slight improvement in wound healing in the hAMSC and hBMSC groups, the differences between the groups were statistically insignificant. In a trans-well coculture model, wound healing migration and transwell migration assays showed that hADSCs were superior to hAMSCs and hBMSCs at promoting human dermal fibroblast (hDF) migration. However, there was no significant difference in fibroblast proliferation between the hAMSC, hBMSC and hADSC groups, as measured by WST assay. Our results also indicated that hDFs cocultured with hADSCs for 48 h significantly upregulated their mRNA expression of the cytokine vascular endothelial growth factor, basic fibroblast growth factor, keratinocyte growth factor and transforming growth factor-β and increased the mRNA and protein levels of type I collagen. Collectively, these data suggest that hADSCs are a potential source of MSCs for therapeutic healing in cutaneous wounds in terms of efficacy, accessibility and availability.
authors.affiliation: Key Laboratory of Cell Biology, Ministry of Public Health of                     China, Department of Stem Cells and Regenerative Medicine, The First Affiliated                     Hospital, China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Liu
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.forename: Ping
authors.initials: P
authors.lastname: Shi
authors.forename: Yide
authors.initials: Y
authors.lastname: Jiang
authors.forename: Xining
authors.initials: X
authors.lastname: Pang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000650:Amnion / Q000166:cytology*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D012867:Skin / Q000166:cytology / Q000293:injuries* / Q000473:pathology; D014945:Wound Healing
chemical_list: D016207:Cytokines