title: Quantitative trait loci mapping in Brassica rapa revealed the structural and functional conservation of genetic loci governing morphological and yield component traits in the A, B, and C subgenomes of Brassica species.
abstract: Brassica rapa is an important crop species that produces vegetables, oilseed, and fodder. Although many studies reported quantitative trait loci (QTL) mapping, the genes governing most of its economically important traits are still unknown. In this study, we report QTL mapping for morphological and yield component traits in B. rapa and comparative map alignment between B. rapa, B. napus, B. juncea, and Arabidopsis thaliana to identify candidate genes and conserved QTL blocks between them. A total of 95 QTL were identified in different crucifer blocks of the B. rapa genome. Through synteny analysis with A. thaliana, B. rapa candidate genes and intronic and exonic single nucleotide polymorphisms in the parental lines were detected from whole genome resequenced data, a few of which were validated by mapping them to the QTL regions. Semi-quantitative reverse transcriptase PCR analysis showed differences in the expression levels of a few genes in parental lines. Comparative mapping identified five key major evolutionarily conserved crucifer blocks (R, J, F, E, and W) harbouring QTL for morphological and yield components traits between the A, B, and C subgenomes of B. rapa, B. juncea, and B. napus. The information of the identified candidate genes could be used for breeding B. rapa and other related Brassica species.
authors.affiliation: Molecular Genetics and Genomics Laboratory, Department of Horticulture, Chungnam National University, Gung-Dong, Daejeon, Republic of Korea.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Li
authors.forename: Nirala
authors.initials: N
authors.lastname: Ramchiary
authors.forename: Vignesh
authors.initials: V
authors.lastname: Dhandapani
authors.forename: Su Ryun
authors.initials: SR
authors.lastname: Choi
authors.forename: Yoonkang
authors.initials: Y
authors.lastname: Hur
authors.forename: Ill-Sup
authors.initials: IS
authors.lastname: Nou
authors.forename: Moo Kyoung
authors.initials: MK
authors.lastname: Yoon
authors.forename: Yong Pyo
authors.initials: YP
authors.lastname: Lim
mesh_terms: D017360:Arabidopsis / Q000145:classification / Q000235:genetics; D001937:Brassica / Q000145:classification* / Q000235:genetics*; D001947:Breeding; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D017343:Genes, Plant; D005819:Genetic Markers; D018895:Microsatellite Repeats; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D016415:Sequence Alignment
chemical_list: D005819:Genetic Markers