title: Inductive effect of phytoglycoprotein (38 kDa) on G₀/G₁ arrest and apoptosis in diethylnitrosamine-treated ICR mice.
abstract: Promotion in the carcinogenic stage is closely related to the growth and proliferation of liver cancer cells. The purpose of this study was whether Styrax japonicum Siebold & Zuccarini (SJSZ) glycoprotein has preventive effect on the growth of hepatocarcinoma cells in diethylnitrosamine (DEN)-induced ICR mice. The study evaluated cell cycle and the activities of cell cycle-related factors [cyclin D1/cyclin dependent kinase (CDK) 4], cell cycle inhibitors (CKIs; p53, p21, and p27), proliferating cell nuclear antigen (PCNA), cytochrome c, Bid, caspase-3, and caspase-9 in DEN-induced ICR mice by flow cytometry, immunoblot analysis and qRT-PCR. The results showed that SJSZ glycoprotein (10 mg/kg, BW) arrested G(0)/G(1) phase and the activity of cyclin D1/CDK4, PCNA, and Bid. However, it induced activities of CKIs, cytochrome c, caspase-3, and caspase-9. Taken together, this present study suggested that SJSZ glycoprotein might be a potent inhibition of hepatic tumor promotion.
authors.affiliation: Molecular Biochemistry Laboratory, Biotechnology Research Institute & Center for the Control of Animal Hazards Using Biotechnology (BK21), Chonnam National University, 300 Yongbong-Dong, Gwang-ju 500-757, South Korea.
authors.forename: Jin
authors.initials: J
authors.lastname: Lee
authors.forename: Kye-Taek
authors.initials: KT
authors.lastname: Lim
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D051023:BH3 Interacting Domain Death Agonist Protein / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D051358:Cyclin-Dependent Kinase 4 / Q000235:genetics / Q000378:metabolism; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism; D045304:Cytochromes c / Q000378:metabolism; D004052:Diethylnitrosamine; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects; D006023:Glycoproteins / Q000494:pharmacology*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010940:Plant Proteins / Q000494:pharmacology*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D032362:Styrax / Q000737:chemistry; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D016588:Anticarcinogenic Agents; D051023:BH3 Interacting Domain Death Agonist Protein; C494815:Bid protein, mouse; C530105:Ccnd1 protein, mouse; D050759:Cyclin-Dependent Kinase Inhibitor p21; D006023:Glycoproteins; D010940:Plant Proteins; D018809:Proliferating Cell Nuclear Antigen; D016159:Tumor Suppressor Protein p53; D019938:Cyclin D1; D050760:Cyclin-Dependent Kinase Inhibitor p27; D004052:Diethylnitrosamine; D045304:Cytochromes c; C495902:Cdk4 protein, mouse; D051358:Cyclin-Dependent Kinase 4; C505409:Casp3 protein, mouse; C506434:Casp9 protein, mouse; D053148:Caspase 3; D053453:Caspase 9