title: Inhibition of xenograft human glioma tumor growth by lentivirus-mediated             gene transfer of alphastatin.
abstract: Angiogenesis is crucial for the development and metastasis of human brain             glioma. Based on our previous successful construction of a lentivirus-mediated             alphastatin (an endogenous angiogenesis inhibitor) gene transfer system and our             findings that alphastatin exhibited potent inhibitory effects on the migration             and differentiation of human umbilical vein endothelial cell lines (HUVECs) induced             by vascular endothelial growth factor (VEGF) or basic fibroblast growth factor             (bFGF) in vitro, here, we investigated the effect of using lentiviral vectors             to overexpress alphastatin in human glioma cells to show whether sustained long-term             expression of alphastatin diminishes tumor growth in a xenograft glioma model.             We found that the transduced glioma cells sustainedly secreted alphastatin, which             did not affect the proliferative ability of the glioma cells. Furthermore, tumor             xenografts treated with the recombinant lentivirus were significantly smaller             compared to the control xenografts and vascularity within the treated tumors was             evidently decreased. Our data suggest that stable expression of alphastatin inhibits             human glioma growth by inhibiting angiogenesis, with a probable mechanism of suppressing             the turnover of VE-cadherin membrane molecules.
authors.affiliation: Department of Neurosurgery, The Second Affiliated Hospital, the Fourth Military Medical University, Xi'an 710038, PR China.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Che
authors.forename: Jianrong
authors.initials: J
authors.lastname: Song
authors.forename: Shiwen
authors.initials: S
authors.lastname: Guo
authors.forename: Weiwen
authors.initials: W
authors.lastname: Wang
authors.forename: Guodong
authors.initials: G
authors.lastname: Gao
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005340:Fibrinogen / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D015316:Genetic Therapy; D005822:Genetic Vectors; D005910:Glioma / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D061307:Human Umbilical Vein Endothelial Cells / Q000821:virology; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D014161:Transduction, Genetic; D047368:Tumor Burden; D000099152:Cadherin 5
chemical_list: D015703:Antigens, CD; D015820:Cadherins; D005340:Fibrinogen; D011994:Recombinant Proteins; D000099152:Cadherin 5; C514422:alphastatin