title: In vivo detection and quantification of chemicals that enhance protein stability.
abstract: We have devised protein-folding sensors that link protein stability to TEM-1 β-lactamase activity. The addition of osmolytes and other compounds with chemical chaperone activity to the growth medium of bacteria containing these sensors increases β-lactamase activity up to 207-fold in a dose-dependent manner. This enables the rapid detection and sensitive quantification of compounds that enhance in vivo protein stability.
authors.affiliation: Howard Hughes Medical Institute, Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: Tsinatkeab T
authors.initials: TT
authors.lastname: Hailu
authors.forename: Linda
authors.initials: L
authors.lastname: Foit
authors.forename: James C A
authors.initials: JC
authors.lastname: Bardwell
mesh_terms: D002241:Carbohydrates / Q000032:analysis*; D002364:Caseins / Q000032:analysis*; D003124:Colorimetry; D004926:Escherichia coli / Q000378:metabolism; D008055:Lipids / Q000032:analysis*; D018832:Molecular Chaperones / Q000378:metabolism; D014674:Plant Proteins, Dietary / Q000032:analysis*; D017510:Protein Folding; D055550:Protein Stability; D001618:beta-Lactamases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002241:Carbohydrates; D002364:Caseins; D008055:Lipids; D018832:Molecular Chaperones; D014674:Plant Proteins, Dietary; C080652:Osmolite; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1