title: Synthesis of maleimide-functionalyzed HPMA-copolymers and in vitro characterization of the aRAGE- and human immunoglobulin (huIgG)-polymer conjugates.
abstract: Herein the synthesis of antibody-polymer conjugates, with a quite narrow dispersity based on the polymer HPMA, are reported. These conjugates are synthesized by coupling antibodies to maleimide-functionalized poly(N-(2-hydroxypropyl)-methacrylamide) (poly-HPMA) copolymers derived through reversible addition-fragmentation chain transfer (RAFT) polymerization of pentafluorophenyl methacrylate via the intermediate step of an activated ester polymer. We develop a protocol that allows the attachment of two different model antibodies, monoclonal anti-RAGE (receptor for advanced glycation end-products) antibody, and polyclonal human immunoglobulin (huIgG). Modification of the antibody and conjugation is monitored by SDS-PAGE electrophoresis. Preserved affinity is demonstrated by Western Blott and cell-uptake analysis, for example, to cells of the immune system.
authors.affiliation: Johannes Gutenberg-Universität Mainz, Institute of Organic Chemistry, Mainz, Germany.
authors.forename: Kristof
authors.initials: K
authors.lastname: Tappertzhofen
authors.forename: Verena V
authors.initials: VV
authors.lastname: Metz
authors.forename: Mario
authors.initials: M
authors.lastname: Hubo
authors.forename: Matthias
authors.initials: M
authors.lastname: Barz
authors.forename: Rolf
authors.initials: R
authors.lastname: Postina
authors.forename: Helmut
authors.initials: H
authors.lastname: Jonuleit
authors.forename: Rudolf
authors.initials: R
authors.lastname: Zentel
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry*; D001665:Binding Sites; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007136:Immunoglobulins / Q000737:chemistry* / Q000378:metabolism; D008301:Maleimides / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000276:immunology* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D007136:Immunoglobulins; D008301:Maleimides; D011109:Polymethacrylic Acids; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; C043592:maleimide; C011533:Duxon