title: Simultaneous optical coherence tomography and autofluorescence microscopy with a single light source.
abstract: We have accomplished simultaneous spectral domain optical coherence tomography (SD-OCT) and autofluorescence (AF) microscopy with a broadband light source centered at 415 nm. The light source was provided by frequency-doubling of an ultra-fast broadband Ti:Sapphire laser. With a bandwidth of 8 nm, the visible SD-OCT achieved a depth resolution of ~12  μm. Since the two imaging modalities are provided by the same group of photons, their images are intrinsically registered. The dual-modal system is capable of providing OCT imaging and molecular contrasts simultaneously. The imaging system was tested on imaging biological samples ex vivo and in vivo.
authors.affiliation: Shanghai Institute of Technology, College of Science, 100 Haiquan Road, Shanghai 201418, China.
authors.forename: Cuixia
authors.initials: C
authors.lastname: Dai
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Liu
authors.forename: Shuliang
authors.initials: S
authors.lastname: Jiao
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D008029:Lighting / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D057054:Molecular Imaging / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013382:Subtraction Technique / Q000295:instrumentation*; D041623:Tomography, Optical Coherence / Q000295:instrumentation*