title: Manganese-enhanced MRI reflects seizure outcome in a model for mesial temporal lobe epilepsy.
abstract: The neurobiological processes resulting in epilepsy, known as epileptogenesis, are incompletely understood. Manganese-enhanced MRI (MEMRI) can potentially aide in this quest as it provides superior tissue contrast, particularly of the hippocampal subregions. This longitudinal study aims to characterise the changes in the hippocampus of the post kainic acid-induced status epilepticus (KASE) rat model of mesial temporal lobe epilepsy using MEMRI in vivo. Serial acquisition of T(1)-weighted MEMRI images were taken before, 2 days and 6 weeks after KASE (10-30 mg/kg, i.p.) in 14 rats and in 11 control rats, while a second cohort of control (N=6) and epileptic animals (N=10) was imaged at 2 months post KASE only. MnCl(2) (50 mM, 10 μl) was administered in the right lateral ventricle 1 day before scanning. Regions of interest were drawn around the hippocampus and several subregions of the hippocampus (CA1, CA3 and dentate gyrus). Markers of epilepsy such as spontaneous recurrent seizures, hippocampal neuronal loss and mossy fiber sprouting were quantified. A persistent increase in MEMRI signal intensity was found in the hippocampus, CA1 and dentate gyrus in the KASE group compared to the control group (ANOVA P<0.05). The intensity signal in the hippocampus and subregions correlated inversely with the frequency of spontaneous recurrent seizures in the chronic epileptic phase, however there was no relationship observed between histopathological changes such as cell loss and mossy fiber sprouting with seizures. This study demonstrates that MEMRI is able to detect imaging changes in the hippocampus during the course of epileptogenesis relevant for seizure expression. These data strongly indicate a relationship between manganese enhancement and spontaneous seizure outcome, suggesting that MEMRI could provide a preclinical biomarker for the severity of epileptogenesis in vivo in animal models.
authors.affiliation: Department of Translational Neuroscience, University of Antwerp, Wilrijk, Belgium. stefanie.dedeurwaerdere@ua.ac.be
authors.forename: S
authors.initials: S
authors.lastname: Dedeurwaerdere
authors.forename: K
authors.initials: K
authors.lastname: Fang
authors.forename: M
authors.initials: M
authors.lastname: Chow
authors.forename: Y-T
authors.initials: YT
authors.lastname: Shen
authors.forename: I
authors.initials: I
authors.lastname: Noordman
authors.forename: L
authors.initials: L
authors.lastname: van Raay
authors.forename: N
authors.initials: N
authors.lastname: Faggian
authors.forename: M
authors.initials: M
authors.lastname: Porritt
authors.forename: G F
authors.initials: GF
authors.lastname: Egan
authors.forename: T J
authors.initials: TJ
authors.lastname: O'Brien
mesh_terms: D000818:Animals; D003287:Contrast Media; D003292:Convulsants / Q000633:toxicity; D004195:Disease Models, Animal; D004833:Epilepsy, Temporal Lobe / Q000139:chemically induced / Q000473:pathology*; D006624:Hippocampus / Q000473:pathology*; D007090:Image Interpretation, Computer-Assisted; D007608:Kainic Acid / Q000633:toxicity; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D008345:Manganese; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000473:pathology*
chemical_list: D003287:Contrast Media; D003292:Convulsants; D008345:Manganese; D007608:Kainic Acid