title: [A family-based transmission disequilibrium test of polymorphisms of endothelial nitric oxide synthase gene in children with orthostatic intolerance].
abstract: OBJECTIVE
  Recent studies have found that the variation of G894T on the region of T786C and 7th exon promoted by endothelial nitric oxide synthase (eNOS) gene is associated with cardiovascular disease. This research explored possible correlations between eNOS gene polymorphisms and orthostatic intolerance (OI) in children through linkage disequilibrium analysis between eNOS genes T786C and G894T and OI.
  
  
  METHODS
  PCR, Macrorestriction Map and other molecular biotechnology were used to determine the genotypes of eNOS/T786C and G894T in 60 OI probands and their parents. Correlation analysis and transmission disequilibrium test (TDT) between T786C, G894T and OI were performed.
  
  
  RESULTS
  There was linkage disequilibrium of eNOS/T786C and G894T gene polymorphisms in the occurrence of childhood OI (P<0.05).
  
  
  CONCLUSIONS
  eNOS genes T786C and G894T may be associated with the pathogenesis of OI.
authors.affiliation: Department of Cardiology, Kaifeng Children's Hospital, Kaifeng, Henan, China.
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Xiong
authors.forename: Zi-Yan
authors.initials: ZY
authors.lastname: Han
authors.forename: Zhen-Hui
authors.initials: ZH
authors.lastname: Han
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.forename: Xi-Qing
authors.initials: XQ
authors.lastname: Gao
authors.forename: Yong
authors.initials: Y
authors.lastname: Gai
authors.forename: Jie
authors.initials: J
authors.lastname: Lian
mesh_terms: D000328:Adult; D002648:Child; D005260:Female; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D054971:Orthostatic Intolerance / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III