title: [Characterization of S gene of a strain of hantavirus isolated from Apodemus peninsulae in Heilongjiang Province].
abstract: In order to study the molecular characterization of the hantavirus isolated from Apodemus peninsulae in Heilongjiang Province, the S gene of a new strain NA33 was amplified, sequenced and analyzed. The results showed that the complete nucleotide sequence of the S gene of NA33 strain was composed of 1 693 nucleotides with TA-rich. The S gene contained one ORF, starting at position 37 and ending at position 1 326, encoding the N protein of 429 amino acid residues, and in line with HTN-based coding. Sequence comparison of the S genes between NA33 and reference hantavirus strains showed that NA33 was more homologous to Amur-like viruses than to the Hantaan (HTN) viruses or the other hantaviruses. Phylogenetic analysis of the amino acid sequence of N proteins showed that NA33 was clustered into the group of Amur-like viruses and was more similar to Far East Russia and Jilin strains isolated from Apodemus peninsulae. The phylogenetic tree indicated a certain degree of host-dependent characteristics and geographical aggregation characteristics of hantanviruses. Furthermore, the amino acid sequence of N protein of NA33 had the conserved amino acid sites of Amur-like viruses. In conclusion, Apodemus peninsulae carried Amur-like viruses in Heilongjiang province and was an important infectious source of hemorrhagic fever with renal syndrome (HFRS).
authors.affiliation: Institute for Viral Disease Control and Prevention, Heilongjiang Provincial Center for Disease Prevention and Control, Harbin 150030, China. hljlfc@163.com
authors.forename: Lu-Fei
authors.initials: LF
authors.lastname: Chen
authors.forename: Shu-Hong
authors.initials: SH
authors.lastname: Chen
authors.forename: Kai-Li
authors.initials: KL
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Li
mesh_terms: D000818:Animals; D002681:China; D006476:Orthohantavirus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D018778:Hantavirus Infections / Q000662:veterinary* / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D051189:Murinae / Q000821:virology*; D010802:Phylogeny; D012376:Rodent Diseases / Q000821:virology*; D017386:Sequence Homology, Amino Acid; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D014759:Viral Envelope Proteins