title: Requirement for both receptor-operated and store-operated calcium entry in N-formyl-methionine-leucine-phenylalanine-induced neutrophil polarization.
abstract: Tissue penetration of neutrophils is a key process in many inflammatory diseases. In response to inflammatory stimuli such as N-formyl-methionine-leucine-phenylalanine (fMLP), neutrophils polarize and migrate towards the chemotactic gradient of the stimulus. Elevated intracellular Ca(2+) concentration is known to play a critical role in neutrophil polarization and migration; however, the exact mechanism remains elusive. Here, we demonstrated that fMLP stimulation caused not only store-operated calcium entry (SOCE), but also receptor-operated calcium entry (ROCE) in neutrophils by using both pharmacological and neutralizing monoclonal antibody approaches. We also investigated neither Rac2 nor Cdc42 activation could take place if either SOCE or ROCE was inhibited. This study thus provides the first evidence for coordination of Ca(2+) influx by SOCE and ROCE to regulate neutrophil polarization.
authors.affiliation: Department of Occupational Health and Occupational Medicine, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China.
authors.forename: Chunqing
authors.initials: C
authors.lastname: Cai
authors.forename: Shihao
authors.initials: S
authors.lastname: Tang
authors.forename: Xubu
authors.initials: X
authors.lastname: Wang
authors.forename: Shaoxi
authors.initials: S
authors.lastname: Cai
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Meng
authors.forename: Wenying
authors.initials: W
authors.lastname: Zou
authors.forename: Fei
authors.initials: F
authors.lastname: Zou
mesh_terms: D002118:Calcium / Q000378:metabolism*; D016764:Cell Polarity; D002478:Cells, Cultured; D002633:Chemotaxis; D006801:Humans; D053858:Metabolic Networks and Pathways; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism; D020744:rac GTP-Binding Proteins / Q000378:metabolism; D000097766:RAC2 GTP-Binding Protein
chemical_list: D002118:Calcium; D009240:N-Formylmethionine Leucyl-Phenylalanine; D020764:cdc42 GTP-Binding Protein; D020744:rac GTP-Binding Proteins; D000097766:RAC2 GTP-Binding Protein