title: Simultaneous production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol using resting cells of the lactate dehydrogenase-deficient recombinant Klebsiella pneumoniae overexpressing an aldehyde dehydrogenase.
abstract: In the present study, the lactate dehydrogenase-deficient (ldhA(-)) recombinant Klebsiella pneumoniae overexpressing an ALDH (KGSADH) was developed and the co-production of 3-HP and PDO from glycerol by this recombinant under resting cell conditions was examined. The new recombinant did not produce any appreciable lactate, which seriously inhibits the production of 3-HP and PDO. The final titers of 3-HP and PDO by the ldhA(-) recombinant strain at 60 h were 252.2 mM and 308.7 mM, respectively, which were improved by approximately 30% and 50%, respectively, compared to those by the counterpart recombinant strain, which was the wild type for ldhA. In addition, after deleting ldhA, the cumulative yield on glycerol and specific production rate of these two metabolites (3-HP and PDO) were enhanced by 41.4% and 52%, respectively.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Pusan National University, San 30, Jangjeon-dong, Geumjeong-gu, Busan 609-735, South Korea.
authors.forename: Vinod
authors.initials: V
authors.lastname: Kumar
authors.forename: Mugesh
authors.initials: M
authors.lastname: Sankaranarayanan
authors.forename: Meetu
authors.initials: M
authors.lastname: Durgapal
authors.forename: Shengfang
authors.initials: S
authors.lastname: Zhou
authors.forename: Yeounjoo
authors.initials: Y
authors.lastname: Ko
authors.forename: Somasundar
authors.initials: S
authors.lastname: Ashok
authors.forename: Ritam
authors.initials: R
authors.lastname: Sarkar
authors.forename: Sunghoon
authors.initials: S
authors.lastname: Park
mesh_terms: D000332:Aerobiosis; D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D019149:Bioreactors / Q000382:microbiology; D005990:Glycerol / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development; D007770:L-Lactate Dehydrogenase / Q000172:deficiency* / Q000378:metabolism; D019344:Lactic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis; D011409:Propylene Glycols / Q000378:metabolism*; D011995:Recombination, Genetic / Q000235:genetics*; D013997:Time Factors
chemical_list: D011409:Propylene Glycols; D019344:Lactic Acid; C041787:1,3-propanediol; C031601:hydracrylic acid; D007770:L-Lactate Dehydrogenase; D000444:Aldehyde Dehydrogenase; D005990:Glycerol