title: Wide-field fast-scanning photoacoustic microscopy based on a water-immersible MEMS scanning mirror.
abstract: By offering images with high spatial resolution and unique optical absorption contrast, optical-resolution photoacoustic microscopy (OR-PAM) has gained increasing attention in biomedical research. Recent developments in OR-PAM have improved its imaging speed, but have to sacrifice either the detection sensitivity or field of view or both. We have developed a wide-field fast-scanning OR-PAM by using a water-immersible microelectromechanical systems (MEMS) scanning mirror (MEMS-OR-PAM). In MEMS-OR-PAM, the optical and acoustic beams are confocally configured and simultaneously steered, which ensures the uniform detection sensitivity. A B-scan imaging speed as high as 400 Hz can be achieved over a 3 mm scanning range. Using the system, we imaged the flow dynamics of both red blood cells and carbon particles in a mouse ear in vivo. Presented results show that MEMS-OR-PAM could be a powerful tool for studying highly dynamic and time-sensitive biological phenomena.
authors.affiliation: Washington University in St. Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, St. Louis, MO 63130, USA.
authors.forename: Junjie
authors.initials: J
authors.lastname: Yao
authors.forename: Chih-Hsien
authors.initials: CH
authors.lastname: Huang
authors.forename: Lidai
authors.initials: L
authors.lastname: Wang
authors.forename: Joon-Mo
authors.initials: JM
authors.lastname: Yang
authors.forename: Liang
authors.initials: L
authors.lastname: Gao
authors.forename: Konstantin I
authors.initials: KI
authors.lastname: Maslov
authors.forename: Jun
authors.initials: J
authors.lastname: Zou
authors.forename: Lihong V
authors.initials: LV
authors.lastname: Wang
mesh_terms: D000818:Animals; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D007909:Lenses; D051379:Mice; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D018613:Microscopy, Confocal / Q000295:instrumentation*; D061088:Photoacoustic Techniques / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014867:Water
chemical_list: D014867:Water