title: Preparation of functional water-soluble low-cytotoxic poly(methacrylate)s with pendant cationic L-lysines for efficient gene delivery.
abstract: In this work, we present the preparation of water-soluble poly(methacrylate)s with pendant cationic L-lysines PHMLs(6-30 K). Plasmid DNA binding affinity as well as particle sizes and zeta potentials of the polyplexes were examined for these PHML vectors, and their cytotoxicities were assayed with HeLa cells by CCK-8 and lactate dehydrogenase kits. Gene transfection efficacy and intracellular uptake of the polyplexes by the PHML vectors were also studied with HeLa cells. As a result, it was revealed that the low cytotoxic PHMLs tended to exhibit gene transfection efficiencies significantly higher than those of the linear structural PLL (15-30 K) control, in particular the molecular weight of a PHML vector remarkably influenced its pDNA binding affinity, transfection efficacy and intracellular uptake of the polyplexes.
authors.affiliation: Laboratory for Polymer Materials, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Sun
authors.forename: Ting
authors.initials: T
authors.lastname: Luo
authors.forename: Ruilong
authors.initials: R
authors.lastname: Sheng
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Shengdian
authors.initials: S
authors.lastname: Chen
authors.forename: Fangzhen
authors.initials: F
authors.lastname: Hu
authors.forename: Amin
authors.initials: A
authors.lastname: Cao
mesh_terms: D001692:Biological Transport; D045744:Cell Line, Tumor; D002470:Cell Survival; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000235:genetics; D008239:Lysine / Q000737:chemistry*; D010957:Plasmids / Q000235:genetics*; D011109:Polymethacrylic Acids / Q000737:chemistry*
chemical_list: D011109:Polymethacrylic Acids; C030613:polymethacrylic acid; D007770:L-Lactate Dehydrogenase; D008239:Lysine