title: Development in the STORM.
abstract: The recent invention of superresolution microscopy has brought up much excitement in the biological research community. Here, we focus on stochastic optical reconstruction microscopy/photoactivated localization microscopy (STORM/PALM) to discuss the challenges in applying superresolution microscopy to the study of developmental biology, including tissue imaging, sample preparation artifacts, and image interpretation. We also summarize new opportunities that superresolution microscopy could bring to the field of developmental biology.
authors.affiliation: Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA. daichi.kamiyama@ucsf.edu
authors.forename: Daichi
authors.initials: D
authors.lastname: Kamiyama
authors.forename: Bo
authors.initials: B
authors.lastname: Huang
mesh_terms: D002450:Cell Communication; D002468:Cell Physiological Phenomena; D022082:Cellular Structures / Q000648:ultrastructure*; D015509:Developmental Biology / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D008856:Microscopy, Fluorescence / Q000379:methods*