title: Isolation, characterization, and expression analysis of CmMLO2 in muskmelon.
abstract: The full-length cDNA sequence of the MLO gene was cloned via SMART-RACE-PCR from muskmelon (Cucumis melo L.), and was designated as CmMLO2 (GenBank Accession No. FJ713542). The gene is 1,710 bp long and encodes a 570-amino acid peptide with a seven-transmembrane domain topology, and is a typical transmembrane protein. Localization analysis in onion epidermal cells showed that CmMLO2-GFP is localized in the plasma membrane. The expression of CmMLO2 gene was analyzed in melon leaf infected with powdery mildew using a quantitative RT-PCR and it was found that CmMLO2 was mainly expressed in melon leaves in a no-tissue-specific pattern. Moreover, CmMLO2 may play a crucial role in the pathogenesis of powdery mildew.
authors.affiliation: Institute of Vegetable, Gansu Academy of Agricultural Science, Lanzhou, 730070, China. chengjn@yeah.net
authors.forename: Hong
authors.initials: H
authors.lastname: Cheng
authors.forename: Weiping
authors.initials: W
authors.lastname: Kong
authors.forename: Dong
authors.initials: D
authors.lastname: Hou
authors.forename: Junfeng
authors.initials: J
authors.lastname: Lv
authors.forename: Xinglin
authors.initials: X
authors.lastname: Tao
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D028463:Cucumis melo / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D042541:Intracellular Space / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D021381:Protein Transport; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D008565:Membrane Proteins