title: Caulobacter daechungensis sp. nov., a stalked bacterium isolated from a eutrophic reservoir.
abstract: A Gram-stain-negative, aerobic, non-spore-forming, curved, rod-shaped bacterium, H-E3-2(T), was isolated from a water sample taken from Daechung Reservoir, Republic of Korea, during the late-blooming period of cyanobacteria. Strain H-E3-2(T) was motile with a single polar flagellum or non-motile (stalked cell). Comparative 16S rRNA gene sequence studies showed the isolate had a clear affiliation with the class Alphaproteobacteria and was most closely related to Caulobacter fusiformis ATCC 15257(T) and Caulobacter mirabilis LMG 24261(T), showing 97.6 and 97.3 % 16S rRNA gene sequence similarity, respectively, and 95.3-96.3 % similarity to all other species of the genus Caulobacter. The predominant ubiquinone was Q-10. The major fatty acids were summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c) and C16 : 0. The G+C content of the genomic DNA of strain H-E3-2(T) was 64.7 mol%. DNA-DNA hybridization values of strain H-E3-2(T) with C. fusiformis ATCC 15257(T) and C. mirabilis LMG 24261(T) were 21.2 and 19.7 %, respectively. Thus, based on the results of polyphasic analysis, it is proposed that strain H-E3-2(T) represents a novel species of the genus Caulobacter, for which the name Caulobacter daechungensis sp. nov. is proposed. The type strain is H-E3-2(T) ( = KCTC 32211(T) = JCM 18689(T)).
authors.affiliation: Environmental Biotechnology Research Center, Korea Research Institute of Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Long
authors.initials: L
authors.lastname: Jin
authors.affiliation: Environmental Biotechnology Research Center, Korea Research Institute of Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Hyung-Gwan
authors.initials: HG
authors.lastname: Lee
authors.affiliation: Environmental Biotechnology Research Center, Korea Research Institute of Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Hee-Sik
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Environmental Biotechnology Research Center, Korea Research Institute of Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Chi-Yong
authors.initials: CY
authors.lastname: Ahn
authors.affiliation: Environmental Biotechnology Research Center, Korea Research Institute of Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: Hee-Mock
authors.initials: HM
authors.lastname: Oh
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D016934:Caulobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000032:analysis; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C024989:coenzyme Q10