title: Replacement of Arg-386 with Gly in dynamin 1 middle domain reduced GTPase activity and oligomer stability in the absence of lipids.
abstract: Dynamin plays an important role in membrane fission during endocytosis, and its middle domain is involved in the formation of functional oligomers. In this study, we found that replacement of Arg-386 with Gly in the middle domain region of dynamin 1 did not affect the intermolecular interactions of dynamin 1 in the presence of phosphatidylserine-liposomes. But, unexpectedly, this variant showed lower guanosine 5'-triphosphatase activity in the absence of phosphatidylserine-liposomes and enhanced monomer formation from oligomers. Our results indicate that GTPase activity in the absence of lipids is important in the dissociation of oligomer complexes, i.e., reduced basal dynamin 1 GTPase activity is associated with instability of dynamin oligomers.
authors.affiliation: Department of Neuropsychiatry, St. Marianna University School of Medicine, Kawasaki, Japan.
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Takahashi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Otomo
authors.forename: Noboru
authors.initials: N
authors.lastname: Yamaguchi
authors.forename: Hideki
authors.initials: H
authors.lastname: Nakashima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyoshi
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001120:Arginine; D034284:Dynamin I / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004705:Endocytosis / Q000235:genetics; D004795:Enzyme Stability / Q000235:genetics; D005998:Glycine / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010718:Phosphatidylserines / Q000378:metabolism; D055503:Protein Multimerization / Q000235:genetics*; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D014168:Transferrin / Q000378:metabolism
chemical_list: D008081:Liposomes; D010718:Phosphatidylserines; D014168:Transferrin; D001120:Arginine; D034284:Dynamin I; D005998:Glycine