title: [Protective effect of paeoniflorin on Abeta25-35 -induced PC12 cell injury].
abstract: OBJECTIVE
  To investigate the protective effect of paeoniflorin on PC12 cell injury induced by Abeta25-35.
  
  
  METHODS
  Abeta25-35-induced PC12 cell injury model was established. The effect of cell proliferation was detected by the MTT method. We also measured the apoptosis with the FITC-Annexin V/PI staining, observed the mitochondrial membrane potential and changes in intracellular calcium using JC-1 and Fluo-3/AM staining respectively, and studied the protein expression of Bax, Bcl-2, cleaved Caspase-3 by Western blotting analysis.
  
  
  RESULTS
  Paeoniflorin could inhibit Abeta25-35-induced PC12 cytotoxicity and apoptosis and show dose dependence. Its protective effect may be realized by increasing mitochondrial membrane potential Bcl-2 protein expression, decreasing Ca(2+) concentration and Bax protein expression and inhibiting activation of apoptosis-related protein Caspase-3.
  
  
  CONCLUSIONS
  Paeoniflorin shows a protective effect on Abeta25-35-induced PC12 cell injury.
authors.affiliation: Department of Pharmacology, Henan University of Science and Technology, Luoyang 471003, China. ylwsy@126.com
authors.forename: Shuying
authors.initials: S
authors.lastname: Wang
authors.forename: Ling
authors.initials: L
authors.lastname: Liu
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D001565:Benzoates / Q000494:pharmacology*; D001952:Bridged-Ring Compounds / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D005960:Glucosides / Q000494:pharmacology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D039821:Monoterpenes; D016716:PC12 Cells; D010446:Peptide Fragments / Q000633:toxicity*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D006027:Glycosides
chemical_list: D016229:Amyloid beta-Peptides; D051017:Apoptosis Regulatory Proteins; D001565:Benzoates; D001952:Bridged-Ring Compounds; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039821:Monoterpenes; D010446:Peptide Fragments; D020011:Protective Agents; C074636:amyloid beta-protein (25-35); C015423:peoniflorin; D006027:Glycosides