title: Engineering cell surfaces for orthogonal selectability.
abstract: Vectors have been constructed that express the chitin-binding domain (ChBD) on eukaryotic cell surfaces. The ChBD is linked to enhanced green fluorescent protein (EGFP) through a protein that spans the plasma membrane. This binding functionality does not have a counterpart in eukaryotes, thereby endowing the modified cell surface with a property that is orthogonal to animal cells.
authors.affiliation: Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Peng
authors.forename: Dae Hee
authors.initials: DH
authors.lastname: Kim
authors.forename: Teresa M
authors.initials: TM
authors.lastname: Jones
authors.forename: Diana I
authors.initials: DI
authors.lastname: Ruiz
authors.forename: Richard A
authors.initials: RA
authors.lastname: Lerner
mesh_terms: D060846:Cell Engineering; D002688:Chitinases / Q000737:chemistry* / Q000378:metabolism; D015870:Gene Expression; D057809:HEK293 Cells; D006801:Humans; D011485:Protein Binding; D015202:Protein Engineering / Q000379:methods*; D014162:Transfection
chemical_list: D002688:Chitinases