title: The Cytotoxicity Mechanism of 6-Shogaol-Treated HeLa Human Cervical Cancer Cells Revealed by Label-Free Shotgun Proteomics and Bioinformatics Analysis.
abstract: Cervical cancer is one of the most common cancers among women in the world. 6-Shogaol is a natural compound isolated from the rhizome of ginger (Zingiber officinale). In this paper, we demonstrated that 6-shogaol induced apoptosis and G2/M phase arrest in human cervical cancer HeLa cells. Endoplasmic reticulum stress and mitochondrial pathway were involved in 6-shogaol-mediated apoptosis. Proteomic analysis based on label-free strategy by liquid chromatography chip quadrupole time-of-flight mass spectrometry was subsequently proposed to identify, in a non-target-biased manner, the molecular changes in cellular proteins in response to 6-shogaol treatment. A total of 287 proteins were differentially expressed in response to 24 h treatment with 15 μM 6-shogaol in HeLa cells. Significantly changed proteins were subjected to functional pathway analysis by multiple analyzing software. Ingenuity pathway analysis (IPA) suggested that 14-3-3 signaling is a predominant canonical pathway involved in networks which may be significantly associated with the process of apoptosis and G2/M cell cycle arrest induced by 6-shogaol. In conclusion, this work developed an unbiased protein analysis strategy by shotgun proteomics and bioinformatics analysis. Data observed provide a comprehensive analysis of the 6-shogaol-treated HeLa cell proteome and reveal protein alterations that are associated with its anticancer mechanism.
authors.affiliation: State Key Laboratory of Natural Medicines, Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Liu
authors.forename: Yong-Bo
authors.initials: YB
authors.lastname: Peng
authors.forename: Lian-Wen
authors.initials: LW
authors.lastname: Qi
authors.forename: Xiao-Lan
authors.initials: XL
authors.lastname: Cheng
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Xu
authors.forename: Le-Le
authors.initials: LL
authors.lastname: Liu
authors.forename: E-Hu
authors.initials: EH
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Li