title: Wet-priming extracorporeal membrane oxygenation device maintains sterility for up to 35 days of follow-up.
abstract: In emergency cases, rapid extracorporeal membrane oxygenation (ECMO) device initialization is able to drastically reduce the incidence of patient morbidity and/or mortality. Pre-assembled and ready-to-use ECMO circuits might save up to 30-60 critical minutes in patient management. Six ECMO circuits (Oxygenator D905 EOS with REVOLUTION™ pump and Sorin PTS) were assembled in the operating room in standard conditions and then placed at 37°C for 35 days in order to evaluate possible contamination and ingrowth of micro-organisms. Every 7 days after ECMO circuit assembly and wet-priming, samples of priming fluid were analyzed to verify the presence/absence of possible common contaminants (Enterobacteriaceae, Staphylococcus aureus and fungi). Moreover, two supplementary circuits, used as positive controls, were deliberately inoculated with a known concentration of a Escherichia coli strain and prime samplings carried out at different time-points to determine bacterial growth rate. Sterility was maintained in the ECMO circuits for up to 35 days.
authors.affiliation: Department of Cardiac, Thoracic and Vascular Science, University of Padova, Italy. fnaso@bio.unipd.it
authors.forename: F
authors.initials: F
authors.lastname: Naso
authors.forename: A
authors.initials: A
authors.lastname: Gandaglia
authors.forename: P
authors.initials: P
authors.lastname: Balboni
authors.forename: F
authors.initials: F
authors.lastname: Zanella
authors.forename: R
authors.initials: R
authors.lastname: Danesin
authors.forename: M
authors.initials: M
authors.lastname: Spina
authors.forename: G
authors.initials: G
authors.lastname: Gerosa
mesh_terms: D015199:Extracorporeal Membrane Oxygenation / Q000295:instrumentation / Q000379:methods; D008567:Membranes, Artificial; D013997:Time Factors
chemical_list: D008567:Membranes, Artificial