title: [Response of soil microbial community to the bioremediation of soil contaminated with PAHs].
abstract: The diversity of bacterial community in soil contaminated with polycyclic aromatic hydrocarbons (PAHs) was investigated during the plant-microbe remediation enhanced by biosurfactant rhamnolips (RH), using the polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) method. The results showed that Shannon-Weaver diversity index was only 3.17 before bioremediation, and increased to 3.24-3.45 after bioremediation, in particular, highest value was found in the treatment of alfalfa (AL) inoculated with arbuscular mycorrhizal fungi (AM) and PAHs-degrading bacteria (DB) among all the treatments. The clustering analysis showed that the similarities of soil bacterial community of AL, AL + RH, AL + AM and AL + AM + RH were above 90%. At the same time, the similarity of AL + DB was much closer to those of the four treatments mentioned above. Additionally, when the bacterial communities of AL + DB + RH, AL + DB + AM and AL + DB + AM + RH were grouped together, the similarities of these three treatments were also higher than 80%. By sequence alignment, it was found that the predominant and characteristic bands in DGGE patterns were closely related with PAHs-degrading bacteria, such as Bacillus, Pseudomonas, Acidobacteria, Sphingmonas, Rhodopseudomonas, Firmicutes, and Methylocytaceae. Application of rhamnolipids in plant-microbe bioremediation not only improved the bioavailability of PAHs, but also had a simultaneous influence on the diversity of soil bacterial community, resulting in the efficient promotion of PAHs removal from soils.
authors.affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China. zhangj79@issas.ac.cn
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Xian-gui
authors.initials: XG
authors.lastname: Lin
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Liu
authors.forename: Rui
authors.initials: R
authors.lastname: Yin
mesh_terms: D001673:Biodegradation, Environmental; D000455:Medicago sativa / Q000382:microbiology*; D038821:Mycorrhizae / Q000502:physiology*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000378:metabolism; D053204:Root Nodules, Plant / Q000502:physiology; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis* / Q000378:metabolism
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants