title: A novel fluorescent sensor for detection of highly reactive oxygen species, and for imaging such endogenous hROS in the mitochondria of living cells.
abstract: A new dye, PTZ-Cy2, based on a hybrid cyanine–phenothiazine platform, is described. Oxidation by highly reactive oxygen species (hROS) involves attack at the thiazine sulfur atom and destruction of the p conjugation of the cyaninemoiety. Dual fluorescence emissions are enhanced dramatically at λ(em) 470 nm and 595 nm, and PTZ-Cy2 thus serves both as an absorbance ratiometric and a fluorescent "off-on" sensor for detecting hROS. Moreover PTZ-Cy2 shows selectivity for hROS over other oxidants, and gives a highly sensitive response to such endogenous species within the mitochondria of living cells.
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.forename: Tong
authors.initials: T
authors.lastname: Wu
authors.forename: Jianfang
authors.initials: J
authors.lastname: Cao
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Mingming
authors.initials: M
authors.lastname: Hu
authors.forename: Shiguo
authors.initials: S
authors.lastname: Sun
authors.forename: Fengling
authors.initials: F
authors.lastname: Song
authors.forename: Jiangli
authors.initials: J
authors.lastname: Fan
authors.forename: Jingyun
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Peng
mesh_terms: D002232:Carbocyanines / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000378:metabolism*; D010640:Phenothiazines / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; D010640:Phenothiazines; D017382:Reactive Oxygen Species; C011203:cyanine dye 2; C031637:phenothiazine