title: Pectobacterium aroidearum sp. nov., a soft rot pathogen with preference for monocotyledonous plants.
abstract: Several pectolytic bacterial strains, mainly isolated from monocotyledonous plants and previously identified as Pectobacterium carotovorum, were thought to belong to a novel species after several taxonomic analyses including DNA-DNA hybridization. In 16S rRNA gene sequence analyses, these strains had a similarity of >97.9 % to the 16S rRNA gene sequence of strains representing six other pectobacterial species and subspecies. These strains, represented by strain SCRI 109(T), also showed some unique chemotaxonomic features and quantitative differences in polar lipids, lipoquinones and fatty acids. A specific feature of strain SCRI 109(T) was the presence of DMK-8 lipoquinone, while the dominant fatty acids were the summed feature 3 (iso-C15 : 0 2-OH/C16 : 1ω7c), the unsaturated fatty acid C18 : 1ω7c and straight chain fatty acids, mainly C16 : 0. The DNA G+C content of strain SCRI 109(T) was 50.2 mol%. The taxonomic status of strain SCRI 109(T) and related strains in 16S rRNA gene sequence, chemotaxonomic, and physiological analyses was corroborated by the distinct clustering of these strains in multi-locus sequence analyses. It is proposed that these strains represent a novel species for which the name Pectobacterium aroidearum sp. nov. is proposed; the type strain is SCRI 109(T) ( = NCPPB 929(T) = LMG 2417(T) = ICMP 1522(T)).
authors.affiliation: Institute for Plant Disease and Plant Protection, Hannover University, D-30419 Hannover, Germany.
authors.forename: Shaza
authors.initials: S
authors.lastname: Nabhan
authors.affiliation: Charlottetown Laboratory, Canadian Food Inspection Agency, 93 Mount Edward Road, Charlottetown, PE, C1A 5T1, Canada.
authors.forename: Solke H
authors.initials: SH
authors.lastname: De Boer
authors.affiliation: Institute for Plant Disease and Plant Protection, Hannover University, D-30419 Hannover, Germany.
authors.forename: Edgar
authors.initials: E
authors.lastname: Maiss
authors.affiliation: Centre for Tropical and Subtropical Agriculture and Forestry, Georg-August-University, D-37077 Gottingen, Germany.|Institute for Plant Disease and Plant Protection, Hannover University, D-30419 Hannover, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Wydra
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019684:Magnoliopsida / Q000382:microbiology*; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D009693:Nucleic Acid Hybridization; D044043:Pectobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S