title: A mesocosm study of fate and effects of CuO nanoparticles on endobenthic species (Scrobicularia plana, Hediste diversicolor).
abstract: The fate and effects of CuO nanoparticles (CuO NPs) were examined in endobenthic species (Scrobicularia plana , Hediste diversicolor), under environmentally realistic conditions in outdoor mesocosms (exposure to Cu at 10 μg L(-1) in particulate (CuO NPs) or soluble salt (CuNO(3)) forms) for 21 days. Labile Cu was determined in water and sediment by using diffusive gradient in thin films. No labile Cu being detected from CuO NPs; the observed effects in invertebrates exposed to CuO NPs were mainly attributed to the toxicity of nanoparticulate rather than dissolved Cu toxicity. Bioaccumulation of CuO NPs was observed in both species. Biomarkers were examined at different levels of biological organization: biochemical markers of defense and damage, biomarkers of genotoxicity (comet assay), and behavioral biomarkers (feeding and burrowing). Behavioral biomarkers, antioxidant defenses (catalase, glutathion S-transferase, metallothionein), and genotoxicity are the most sensitive tools to highlight the effect of soluble or nanoparticulate metal forms. Concerning other biomarkers of defense (superoxide dismutase, lactate dehydrogenase, laccase) and damage (thiobarbituric acid reactive substances, acetylcholinesterase, acid phosphatase), no significant effects were detected. This experiment shows the suitability of mesocosms for studying the environmental effects of nanoparticles.
authors.affiliation: Groupe Mer, Molécules, Santé (MMS), Université de Nantes et Université Catholique de l'Ouest, Angers, France.
authors.forename: Pierre-Emmanuel
authors.initials: PE
authors.lastname: Buffet
authors.forename: Marion
authors.initials: M
authors.lastname: Richard
authors.forename: Fanny
authors.initials: F
authors.lastname: Caupos
authors.forename: Aurore
authors.initials: A
authors.lastname: Vergnoux
authors.forename: Hanane
authors.initials: H
authors.lastname: Perrein-Ettajani
authors.forename: Andrea
authors.initials: A
authors.lastname: Luna-Acosta
authors.forename: Farida
authors.initials: F
authors.lastname: Akcha
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Amiard
authors.forename: Claude
authors.initials: C
authors.lastname: Amiard-Triquet
authors.forename: Marielle
authors.initials: M
authors.lastname: Guibbolini
authors.forename: Christine
authors.initials: C
authors.lastname: Risso-De Faverney
authors.forename: Helene
authors.initials: H
authors.lastname: Thomas-Guyon
authors.forename: Paul
authors.initials: P
authors.lastname: Reip
authors.forename: Agnieska
authors.initials: A
authors.lastname: Dybowska
authors.forename: Deborah
authors.initials: D
authors.lastname: Berhanu
authors.forename: Eugenia
authors.initials: E
authors.lastname: Valsami-Jones
authors.forename: Catherine
authors.initials: C
authors.lastname: Mouneyrac
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D015415:Biomarkers / Q000378:metabolism; D049872:Bivalvia / Q000187:drug effects*; D020552:Comet Assay; D003300:Copper / Q000633:toxicity*; D004249:DNA Damage; D017753:Ecosystem; D004784:Environmental Monitoring; D005602:France; D053768:Metal Nanoparticles / Q000633:toxicity* / Q000648:ultrastructure; D011077:Polychaeta / Q000187:drug effects*
chemical_list: D015415:Biomarkers; D003300:Copper; C030973:cupric oxide