title: [The effect of compressive stress on the factors for liver regeneration in hepatocytes Chang cell line].
abstract: OBJECTIVE
  To investigate the effect of compressive stress on the factors for liver regeneration including NF-kappaB, signal transducers and activators of transcription 3 (STATS), c-fos and c-jun in hepatocytes Chang cell line.
  
  
  METHODS
  Human hepatocytes Chang cell line were subjected to compressive stress at 1000 microstain or 2000 microstain, expression of NF-kappaB P65, p-STAT3, c-fos and c-jun were detected by Western blot or RT-PCR at 30 min, 1 h, 2 h, 3 h, 4 h, 6 h after application of compressive stress to indicate the priming of hepatocytes proliferation.
  
  
  RESULTS
  Enhanced expressions of NF-kappaB P65 and p-STAT3 were observed in hepatocytes under compressive stress indicated by Western blot, the magnitude of compressive stress loaded significantly affected the level of expression of NF-kappaB P65 at 2 h (P = 0.046) and p-STAT3 at 1 h (P = 0.039), the peak of expression of p-STAT3 was at 30 minutes after stress-loading while NF-kappaB P65 was at 1 hour; RT-PCR showed that expression of c-fos was not significantly different between 1000 microstain and 2000 microstain at each time point, and expression of c-jun was significantly different at 30 minutes (P = 0.026), 1 h (P = 0.031), 2 h (P = 0.033) after compressive stress loading.
  
  
  CONCLUSIONS
  These results indicate that compressive stress may play an important role in initiating the process of liver regeneration.
authors.affiliation: Department of Pathology, Medical and Nursing College of Chengdu University, Chengdu 610106, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Wan
authors.forename: Guang-Ya
authors.initials: GY
authors.lastname: Xu
authors.forename: Chang-Quan
authors.initials: CQ
authors.lastname: Zhang
authors.forename: Gao-Feng
authors.initials: GF
authors.lastname: Bian
authors.forename: Zhen-Zhen
authors.initials: ZZ
authors.lastname: Liu
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Wu
mesh_terms: D002460:Cell Line; D019245:Compressive Strength / Q000502:physiology*; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008115:Liver Regeneration / Q000502:physiology*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D013314:Stress, Mechanical; D051996:Transcription Factor RelA / Q000378:metabolism*
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D051996:Transcription Factor RelA