title: Visfatin affects redox adaptative responses and proliferation in Me45 human malignant melanoma cells: an in vitro study.
abstract: Visfatin has recently been established as a novel adipokine that is predominantly expressed in subcutaneous and visceral fat. Only few studies have investigated the effect of visfatin on prostate, breast, ovarian cancer as well as on astrocytoma cell biology. There have been no previous studies on antioxidative enzyme activities, proliferation processes or levels of DNA damage in malignant melanoma cells in response to visfatin stimulation. Here, we report that visfatin increases activity of selected antioxidative enzymes (SOD, CAT, GSH-Px) in culture supernatants of Me45 human malignant melanoma cells. Our findings suggest that visfatin triggers a redox adaptation response, leading to an upregulation of antioxidant capacity along with decreased levels of the lipid peroxidation process in Me45 melanoma cells. Moreover, visfatin led to a significantly increased proliferation rate in the study using the [(3)H]thymidine incorporation method. Unlike insulin, visfatin-induced melanoma cell proliferation is not mediated by an insulin receptor. Better understanding of the role of visfatin in melanoma redox states may provide sound insight into the association between obesity-related fat adipokines and the antioxidant defense system in vitro in melanoma progression.
authors.affiliation: Department of Physiology, Medical University of Silesia, Zabrze, Poland. rbuldak@sum.edu.pl
authors.forename: Rafał Jakub
authors.initials: RJ
authors.lastname: Bułdak
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Bułdak
authors.forename: Renata
authors.initials: R
authors.lastname: Polaniak
authors.forename: Michał
authors.initials: M
authors.lastname: Kukla
authors.forename: Ewa
authors.initials: E
authors.lastname: Birkner
authors.forename: Robert
authors.initials: R
authors.lastname: Kubina
authors.forename: Agata
authors.initials: A
authors.lastname: Kabała-Dzik
authors.forename: Anna
authors.initials: A
authors.lastname: Duława-Bułdak
authors.forename: Krystyna
authors.initials: K
authors.lastname: Żwirska-Korczala
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects*; D000704:Analysis of Variance; D000975:Antioxidants; D002374:Catalase / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008545:Melanoma / Q000201:enzymology* / Q000235:genetics; D054409:Nicotinamide Phosphoribosyltransferase / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects*; D018709:Statistics, Nonparametric; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D054409:Nicotinamide Phosphoribosyltransferase