title: [Effects of transplantation of human umbilical cord blood mononuclear cells, expressing VEGF and FGF2 genes, into the area of spinal cord traumatic lesion].
abstract: Effects of immediate single transplantation of human umbilical cord blood mononuclear cells (UCB-MC) transfected with recombinant vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF2) genes into the area of injury were studied on the model of rat spinal cord dosed contusion at TVIII level. UCB-MC transfected with EGFP-N2 plasmid were transplanted into the rats of the control group under similar conditions. The presence of EGFP- labeled cells were traced in white matter during 21 days after transplantation at a distance no less than 10 mm in rostral and caudal directions from the nearest point of the injection. By 30 days after the transplantation of UCB-MC transfected with pBud-VEGF-FGF2 plasmid, the cross-sectional area of sparing grey matter increased by more than 60% at a distance of 3 mm from the epicenter of injury. By that time, in the animals of this group, the number of perivascular cells expressing beta receptor of platelet-derived growth factor (PDGFbetaR) was increased by an average of 30% in the outer zones of white matter 1.5 cm from the injury epicenter. Delivery of the therapeutic genes VEGF and FGF2 to the damaged region and their expression in cell carriers stimulates vascularization and post-traumatic spinal cord regeneration.
authors.forename: G F
authors.initials: GF
authors.lastname: Shaĭmardanova
authors.forename: Ia O
authors.initials: IaO
authors.lastname: Mukhamedshina
authors.forename: A A
authors.initials: AA
authors.lastname: rizvanov
authors.forename: I I
authors.initials: II
authors.lastname: Salafutdinov
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Chelyshev
mesh_terms: D000818:Animals; D036101:Cord Blood Stem Cell Transplantation; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D008297:Male; D018919:Neovascularization, Physiologic; D051381:Rats; D013119:Spinal Cord Injuries / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D058630:Spinal Cord Regeneration; D014183:Transplantation, Heterologous; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C485184:enhanced green fluorescent protein; D016222:Fibroblast Growth Factor 2; D049452:Green Fluorescent Proteins