title: Tomato extract and the carotenoids lycopene and lutein improve endothelial function and attenuate inflammatory NF-κB signaling in endothelial cells.
abstract: OBJECTIVE
  In our previous research the antihypertensive properties of lycopene-containing tomato oleoresin have been revealed. The present study was aimed to assess if oleoresin interferes in the inflammatory signalling in endothelial cells, imitating reduction of inflammatory processes in the vessel wall and in this way to propose the mechanism for the reduction of blood pressure by oleoresin.
  
  
  RESULTS
  A wide number of functional and inflammatory markers were investigated in two cultured endothelial cell models [EA.hy926 and human umbilical vein endothelial cell (HUVEC)], exposed to oleoresin and carotenoids lycopene and lutein. All the carotenoids significantly improved basic endothelial function as measured by increased nitric oxide and decreased endothelin (ET-1) release. They were effective in attenuation of inflammatory nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signalling: decrease of tumour necrosis factor-alpha (TNF-α)-induced leukocytes adhesion, expression of adhesion molecules inter-cellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1), and nuclear translocation of NF-κB components as well as some revert of inhibitor of kappa B (IκB) ubiquitination. In addition, the carotenoids were able to inhibit NF-κB activation in transfected endothelial cells. When combined with lutein, oleoresin exerted synergistic effect on preclusion of leukocytes adhesion.
  
  
  CONCLUSIONS
  Prevention of over-expression of adhesion molecules through inhibition of NF-κB signalling may be one of the main mechanisms driving carotenoids to attenuate inflammatory leukocyte adhesion to endothelium. This is the first profound study on the mechanisms involved in the positive action of natural tomato products in endothelial cells.
authors.affiliation: Soroka University Hospital, Faculty of Health Sciences, Ben-Gurion University, Beer Sheva, Israel.
authors.forename: Anna
authors.initials: A
authors.lastname: Armoza
authors.forename: Yulia
authors.initials: Y
authors.lastname: Haim
authors.forename: Asher
authors.initials: A
authors.lastname: Bashiri
authors.forename: Asher
authors.initials: A
authors.lastname: Basiri
authors.forename: Talya
authors.initials: T
authors.lastname: Wolak
authors.forename: Esther
authors.initials: E
authors.lastname: Paran
mesh_terms: D002338:Carotenoids / Q000494:pharmacology*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D014975:Lutein / Q000494:pharmacology*; D000077276:Lycopene; D018551:Solanum lycopersicum / Q000737:chemistry*; D016328:NF-kappa B / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D016328:NF-kappa B; D010936:Plant Extracts; D002338:Carotenoids; D000077276:Lycopene; D014975:Lutein