title: Proteomic study reveals downregulation of apolipoprotein A1 in plasma of poorly controlled diabetes: a pilot study.
abstract: Proteomic approaches aid in gaining a better understanding of the pathophysiology of diabetic complications. In view of this, differential protein expression in diabetic plasma samples was studied by a combination of proteomic and western blot analyses. Diabetic plasma samples were categorized based on glycated haemoglobin levels as controlled diabetes (CD; 7-8%), poorly controlled diabetes (PCD; >8%) and non-diabetic control (ND;<6.4%). Two-dimensional electrophoresis and liquid chromatography‑mass spectrometry revealed differential expression of proteins including upregulation of fibrinogen and haptoglobin and downregulation of vitamin D binding protein, α-1-antitrypsin, transthyretin and apolipoprotein A1 (Apo A1) in diabetic compared with non-diabetic plasma samples. Amongst these proteins, Apo A1 downregulation was prominent in PCD. Downregulation of Apo A1 may serve as an early predictive marker of diabetic complications.
authors.affiliation: Proteomics Facility, Division of Biochemical Sciences, Council                     of Scientific and Industrial Research, National Chemical Laboratory, Pune                     411008, India.
authors.forename: Hemangi S
authors.initials: HS
authors.lastname: Bhonsle
authors.forename: Arvind M
authors.initials: AM
authors.lastname: Korwar
authors.forename: Ashok D
authors.initials: AD
authors.lastname: Chougale
authors.forename: Sachin S
authors.initials: SS
authors.lastname: Kote
authors.forename: Nitin L
authors.initials: NL
authors.lastname: Dhande
authors.forename: Kishore M
authors.initials: KM
authors.lastname: Shelgikar
authors.forename: Mahesh J
authors.initials: MJ
authors.lastname: Kulkarni
mesh_terms: D016632:Apolipoprotein A-I / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D003920:Diabetes Mellitus / Q000378:metabolism* / Q000473:pathology; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D013058:Mass Spectrometry; D010865:Pilot Projects; D020543:Proteome / Q000032:analysis*
chemical_list: D016632:Apolipoprotein A-I; D006442:Glycated Hemoglobin; D020543:Proteome