title: [Application of TRUST, TP-ELISA and TPPA in the serologic diagnosis of syphilis].
abstract: OBJECTIVE
  To investigate the application value of toluidine red unheated serum test (TRUST), treponema pallidum enzyme-linked immunosorbent assay (TP-ELISA) and treponema pallidum particle agglutination assay (TPPA) in the serologic diagnosis of syphilis.
  
  
  METHODS
  We collected serum samples from 12 622 inpatients, 2 253 outpatients and 1 500 subjects in the medical examination center, and screened them by TRUST and TP-ELISA, followed by quantitative tests using TRUST and TPPA.
  
  
  RESULTS
  Among the 16 375 cases, clinically diagnosed syphilis was confirmed in 384, with a positive rate of 100% either by TRUST (1:2 -1 :16) or TPPA (1:80 - 1:2 560), and 92.2% by TP-ELISA.
  
  
  CONCLUSIONS
  TP-ELISA and TRUST should be simultaneously used for syphilis screening, but not TP-ELISA alone, and the results should be confirmed by TPPA. The combination of the three methods plays a valuable role in reducing the misdiagnosis and missed diagnosis, and contributes to the early and accurate diagnosis of syphilis.
authors.affiliation: Department of Clinical Laboratory, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214023, China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Wang
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.forename: Jing-Feng
authors.initials: JF
authors.lastname: Yu
authors.forename: Chen-Yue
authors.initials: CY
authors.lastname: Xiao
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008875:Middle Aged; D013587:Syphilis / Q000097:blood* / Q000175:diagnosis*; D013588:Syphilis Serodiagnosis / Q000379:methods*; D014210:Treponema pallidum; D055815:Young Adult