title: Prevalence of IL-1β +3954 and IL-1α-889 polymorphisms in the Lebanese population and its association with the severity of adult chronic periodontitis.
abstract: Pro-inflammatory cytokines, i.e., IL-1 mediate the inflammatory response and are genetically regulated in periodontal diseases. Strong association was found between the composite genotype allele 2 of IL-1β+3954 and IL-1α-889 and severe chronic periodontitis. The aim of this study is to determine the prevalence of IL-1β+3954 and IL-1α-889 polymorphism in a group of Lebanese individuals of homogeneous ethnicity and the possible association between genotype positive individuals and the severity of periodontal disease. One hundred and fifty-seven patients aged 53.29±13.13 years participated in the study. Subjects were classified as follows: 1) healthy subjects with no attachment loss >1mm and no clinical signs of gingival or periodontal inflammation; 2) diseased subjects with mild periodontitis (less than 15‰ of global periodontal bone loss); 3) subjects with moderate periodontitis (less than 4 interproximal sites with bone loss = or >50 percent and mean bone loss between 15 and 30%); 4) subjects with severe periodontitis (more than 7 interproximal sites with >50% bone loss and mean bone loss >35‰). Blood samples were taken and analyzed for polymorphism in the IL-1α gene at position +4845 and in the IL-1beta gene at position +3953. Statistical analysis was performed using chi-square test, Fisher Exact test, and ANOVA followed by Bonferroni multiple comparisons. The prevalence of genotype-positive subjects was 52.3‰ in the healthy control group and 42 ‰ in the diseased group. Positive genotype heterozygous of allele 1 and 2 for IL-1β+3954 and IL-1α-889 did not represent in this study a major risk for chronic periodontitis (p=0.590). Only subjects homozygous for allele2 of the IL-1β+3954 and IL-1α-889 were significantly more at risk for severe periodontitis with OR of 51.42.
authors.affiliation: Department of Periodontology, Saint-Joseph University, Beirut, Lebanon.
authors.forename: G
authors.initials: G
authors.lastname: Tawil
authors.forename: F A
authors.initials: FA
authors.lastname: Akl
authors.forename: M F
authors.initials: MF
authors.lastname: Dagher
authors.forename: W
authors.initials: W
authors.lastname: Karam
authors.forename: I
authors.initials: I
authors.lastname: Abdallah Hajj Hussein
authors.forename: A
authors.initials: A
authors.lastname: Leone
authors.forename: A R
authors.initials: AR
authors.lastname: Jurjus
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D055113:Chronic Periodontitis / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D053582:Interleukin-1alpha / Q000235:genetics*; D053583:Interleukin-1beta / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic
chemical_list: D053582:Interleukin-1alpha; D053583:Interleukin-1beta