title: [Change of Th22 cells in peripheral blood of patients with primary immune thrombocytopenia and clinical implication].
abstract: OBJECTIVE
  To investigate the change of Th22 cells in the peripheral blood of the patients with primary immune thrombocytopenia (ITP) and evaluate the significance of Th22 cells in ITP.
  
  
  METHODS
  The proportion of Th22 cells in the peripheral blood of ITP patients before therapy (group 1),  ITP patients in complete response after therapy (ITP-CR,  group 2) and healthy donors (group 3) was evaluated by flow cytometry. The cytokines IL-22,  TGF-β,  TNF-α and IL-6 of each group were measured by ELISA. The level of IL-22 mRNA of each group was examined by RT-PCR.
  
  
  RESULTS
  The proportion of Th22 cells and the levels of IL-22,    TNF-α,  IL-6 and IL-22 mRNA in group l and group 2 were significantly higher than those in group 3 (P<0.01). The proportion of Th22 cells and the levels of IL-22,  TNF-α,  IL-6 and IL-22 mRNA in group 2 were lower than those in group 1(P<0.05). But the level of TGF-β in group l [(3.27±1.02) ng/L] and group 2 [(5.41±1.69) ng/L]  was significantly lower than that in group 3 [(9.65±2.78) ng/L] (P<0.01),  and the level of TGF-β in group 1 was lower than that in group 2 (P<0.05).
  
  
  CONCLUSIONS
  In ITP patients,  the number of Th22 cells and the levels of TNF-α and IL-6 increase,  and the level of TGF-β decrease.
authors.affiliation: Department of Hematology, Huaian Second People's Hospital, Xuzhou Medical College, Huaian 223002, China.
authors.forename: Li-min
authors.initials: LM
authors.lastname: Liu
authors.forename: Xing-xia
authors.initials: XX
authors.lastname: Zhang
authors.forename: Guang-sheng
authors.initials: GS
authors.lastname: Zhao
authors.forename: Ye-jun
authors.initials: YJ
authors.lastname: Si
authors.forename: Guo-qiang
authors.initials: GQ
authors.lastname: Lin
authors.forename: Yan-ming
authors.initials: YM
authors.lastname: Zhang
authors.forename: Guang-sheng
authors.initials: GS
authors.lastname: He
authors.forename: De-pei
authors.initials: DP
authors.lastname: Wu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood / Q000235:genetics; D007378:Interleukins / Q000097:blood / Q000235:genetics; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology; D013921:Thrombocytopenia / Q000097:blood* / Q000235:genetics / Q000276:immunology; D016212:Transforming Growth Factor beta / Q000097:blood / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000235:genetics; D055815:Young Adult; D000097043:Interleukin-22
chemical_list: D016753:Interleukin-10; D015850:Interleukin-6; D007378:Interleukins; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D000097043:Interleukin-22