title: [Co-delivery of exogenous protein and DNA into mammalian cells with Lactococcus lactis].
abstract: OBJECTIVE
  To develop a co-delivery system of exogenous protein and DNA into mammalian cells using Lactococcus lactis (L. lactis).
  
  
  METHODS
  We constructed E.coli-L.lactis shuttle plasmid,  pMG36e-RFP/eGFP,  containing a L.lactis expression cassette with the cDNA of red fluorescent protein (RFP) gene and a eukaryotic expression cassette with the cDNA of enhanced green fluorescent protein (eGFP),  then electrotransformated it into L.lactis cells,  and co-cultured with 293T cells to evaluate the role of the system in the improvement of gene delivering efficiency after L.lactis cells were pretreated with glycine. RFP and GFP  expressed in 293T cells was monitored by fluorescence microscopy.
  
  
  RESULTS
  Fluorescence microscopy revealed that the RFP and GFP  was expressed in 293T cells.
  
  
  CONCLUSIONS
  The co-delivery system of protein and DNA into mammalian cells using L.lactis was constructed successfully.
authors.affiliation: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,  College of Life Science and Technology,  Xinjiang University,  Urumqi 830046,  China.
authors.forename: Yi-jie
authors.initials: YJ
authors.lastname: Li
authors.forename: Huan-huan
authors.initials: HH
authors.lastname: Liu
authors.forename: Fu-chun
authors.initials: FC
authors.lastname: Zhang
mesh_terms: D015870:Gene Expression; D018014:Gene Transfer Techniques / Q000295:instrumentation*; D005822:Genetic Vectors / Q000235:genetics* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics
chemical_list: D049452:Green Fluorescent Proteins