title: Modulation of pro-inflammatory mediators in LPS-stimulated human periodontal ligament cells by chitosan and quaternized chitosan.
abstract: The aim of this study was to evaluate the effects of chitosan and quaternized chitosan (HTCC) modulate IL-1β and TNF-α in LPS-stimulated human periodontal ligament cells (HPDLCs). MTT assay revealed that chitosan stimulated the proliferation of HPDLCs. However, HTCC inhibited the proliferation of HPDLCs at concentrations of 1000 and 100 μg/mL more than the control, especially after 5d (P<0.001). ELISA assay exhibited that chitosan inhibited the production of IL-1β and TNF-α at 24, 48 and 72 h. IL-1β and TNF-α secreted by HPDLCs with LPS and treated with 1000 μg/mL of HTCC significantly increased compared to both the control and the chitosan group (P<0.001). The bioactive role for bFGF in modulating the responses of HPDLCs cells to LPS via inhibiting IL-1β and TNF-α production was demonstrated. All results were necessary to enhance our understanding of the biomedical properties of chitosan and HTCC for modulation of pro-inflammatory mediators.
authors.affiliation: The Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong 266001, PR China. jqx_1@163.com
authors.forename: Qiuxia
authors.initials: Q
authors.lastname: Ji
authors.forename: Jing
authors.initials: J
authors.lastname: Deng
authors.forename: Xinbo
authors.initials: X
authors.lastname: Yu
authors.forename: Quanchen
authors.initials: Q
authors.lastname: Xu
authors.forename: Hong
authors.initials: H
authors.lastname: Wu
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Pan
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000037:antagonists & inhibitors / Q000378:metabolism; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D010510:Periodontal Diseases / Q000188:drug therapy / Q000378:metabolism; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D018836:Inflammation Mediators; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D005346:Fibroblast Growth Factors; D048271:Chitosan