title: A numerical model of EMT6/Ro spheroid dynamics under irradiation: calibration and estimation of the underlying irradiation-induced cell survival probability.
abstract: We present extensions to our quasi-2D cellular automata spheroid model that add a cellular kinetics module together with an irradiation and repair module. Significantly, our approach is not based on the Linear Quadratic (LQ) model, instead, we propose a simple two-parameter, algorithmic model which captures the essential biological features of irradiation-induced cell death, repair and associated cell cycle delays. This approach allows us to estimate directly the underlying irradiation-induced cell survival probability. We present the calibration of this extended model both with and without the application of single irradiation doses to the commonly studied (in vitro) EMT6/Ro (mammary carcinoma) cell line. A comparison of the estimated underlying cell survival probability with the in vitro survival probability data confirms the expected differences in the measures.
authors.affiliation: Department of Economics, Monash University, Clayton, 3800 VIC, Australia. simon.angus@monash.edu
authors.forename: Simon D
authors.initials: SD
authors.lastname: Angus
authors.forename: Monika Joanna
authors.initials: MJ
authors.lastname: Piotrowska
mesh_terms: D000465:Algorithms; D000818:Animals; D002453:Cell Cycle / Q000528:radiation effects*; D016923:Cell Death / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D008954:Models, Biological; D011839:Radiation, Ionizing; D018874:Spheroids, Cellular / Q000166:cytology / Q000378:metabolism*