title: Functional analysis of potato genes involved in quantitative resistance to Phytophthora infestans.
abstract: The most significant threat to potato production worldwide is the late blight disease, which is caused by the oomycete pathogen Phytophthora infestans. Based on previous cDNA microarrays and cDNA-amplified fragment length polymorphism analysis, 63 candidate genes that are expected to contribute to developing a durable resistance to late blight were selected for further functional analysis. We performed virus-induced gene silencing (VIGS) to these candidate genes on both Nicotiana benthamiana and potato, subsequently inoculated detached leaves and assessed the resistance level. Ten genes decreased the resistance to P. infestans after VIGS treatment. Among those, a lipoxygenase (LOX; EC 1.13.11.12) and a suberization-associated anionic peroxidase affected the resistance in both N. benthamiana and potato. Our results identify genes that may play a role in quantitative resistance mechanisms to late blight.
authors.affiliation: Key Laboratory of Horticultural Plant Biology (Huazhong Agricultural University), Ministry of Education, National Center for Vegetable Improvement (Central China), Potato Engineering and Technology Research Center of Hubei Province, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Tian
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Vivianne G A A
authors.initials: VG
authors.lastname: Vleeshouwers
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Shi
authors.forename: Conghua
authors.initials: C
authors.lastname: Xie
mesh_terms: D016960:Agrobacterium tumefaciens; D060467:Disease Resistance / Q000235:genetics*; D020868:Gene Silencing; D017343:Genes, Plant; D056726:Genetic Association Studies; D054884:Host-Pathogen Interactions; D008084:Lipoxygenase / Q000235:genetics / Q000502:physiology; D058977:Molecular Sequence Annotation; D010544:Peroxidases / Q000235:genetics / Q000502:physiology; D055750:Phytophthora infestans / Q000502:physiology*; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology; D010942:Plant Viruses / Q000235:genetics; D011198:Solanum tuberosum / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D014026:Nicotiana / Q000235:genetics / Q000382:microbiology
chemical_list: D010940:Plant Proteins; D010544:Peroxidases; C120494:anionic peroxidase; D008084:Lipoxygenase