title: Karyotype reconstruction modulates the sensitivity of barley genome to radiation-induced DNA and chromosomal damage.
abstract: The potential of cytologically reconstructed barley line D-2946 to cope with the major lesions that hamper genome integrity, namely DNA single- and double-strand breaks was investigated. Strand breaks induced by γ-rays and Li ions were assessed by neutral and alkaline comet assay. Repair capacity after bleomycin treatment was evaluated by agarose gel electrophoresis under neutral and alkaline conditions. Frequencies of radiation-induced chromosome aberrations were also determined. Results indicate that radiation-mediated constitutive rearrangement of the chromosome complement has led to a substantial modulation of the sensitivity of barley genome towards DNA strand breaks, produced by ionising radiation, Li ion implantation and bleomycin in an agent-specific manner, as well as of the clastogenic response to γ-rays. Based on these findings, reconstructed barley karyotype D-2946 can be considered a candidate radio-sensitive line with reduced ability to maintain genome integrity with respect to both DNA and chromosomal damage.
authors.affiliation: Institute of Plant Physiology and Genetics, Department of Molecular Genetics, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria. molgen@bas.bg
authors.forename: Lubomir
authors.initials: L
authors.lastname: Stoilov
authors.forename: Mariyana
authors.initials: M
authors.lastname: Georgieva
authors.forename: Vasilissa
authors.initials: V
authors.lastname: Manova
authors.forename: Luxiang
authors.initials: L
authors.lastname: Liu
authors.forename: Kostadin
authors.initials: K
authors.lastname: Gecheff
mesh_terms: D001761:Bleomycin / Q000633:toxicity; D015139:Blotting, Southern; D002869:Chromosome Aberrations / Q000528:radiation effects*; D032461:Chromosomes, Plant / Q000235:genetics; D020552:Comet Assay; D004249:DNA Damage / Q000528:radiation effects*; D018744:DNA, Plant / Q000528:radiation effects; D004587:Electrophoresis, Agar Gel; D005720:Gamma Rays; D018745:Genome, Plant; D018525:Germination / Q000528:radiation effects; D001467:Hordeum / Q000235:genetics*; D059785:Karyotype; D007700:Kinetics; D009153:Mutagens / Q000633:toxicity; D018517:Plant Roots / Q000528:radiation effects; D011836:Radiation Tolerance
chemical_list: D018744:DNA, Plant; D009153:Mutagens; D001761:Bleomycin