title: Effects of urinary and recombinant gonadotropins on in vitro maturation outcomes of mouse preantral follicles.
abstract: Gonadotropins including follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play a crucial role in human-assisted reproduction techniques. Despite wide use of recombinant gonadotropins in clinical practice, the efficacy of urinary gonadotropins and the dosage of LH component have not yet been elucidated. This study was designed to investigate the difference of follicle culture outcomes according to various compositions of gonadotropins during in vitro culture of mouse preantral follicles. Ovaries were obtained from the 14-day-old C57BL/6 mice, and preantral follicles were isolated and cultured in culture media supplemented with human menopausal gonadotropin (hMG) 200 mIU/mL (group 1), recombinant FSH and LH (rFSH + rLH) 200 mIU/mL each (group 2), rFSH 200 mIU/mL + rLH 100 mIU/mL (group 3), or rFSH 200 mIU/mL + rLH 20 mIU/mL (group 4). Follicle survival rate was significantly lower in group 4. Antral follicles in lower doses of LH (groups 3, 4) showed a statistically significant larger diameter and tended to have a higher antral formation rate. However, follicles in group 1 tended to have a higher oocyte maturation rate. Estradiol concentration from conditioned media from 2:1 FSH/LH (group 3) was significantly higher than those from 1:1 FSH/LH (group 2) or 10:1 FSH/LH (group 4). Half dose of rLH to rFSH facilitated upregulation of growth differentiation factor 9 (Gdf9) expression in granulosa cells when compared to 1:1 FSH/LH or 10:1 FSH/LH. Conclusively, recombinant gonadotropins provided a comparable condition to hMG, and half dose of rLH to rFSH seems to be more suitable for follicular development during in vitro culture.
authors.affiliation: Department of Obstetrics and Gynecology, College of Medicine, Seoul, South Korea.
authors.forename: Kyung Eui
authors.initials: KE
authors.lastname: Park
authors.forename: Seung-Yup
authors.initials: SY
authors.lastname: Ku
authors.forename: Kyung Cheon
authors.initials: KC
authors.lastname: Jung
authors.forename: Hung Ching
authors.initials: HC
authors.lastname: Liu
authors.forename: Yoon Young
authors.initials: YY
authors.lastname: Kim
authors.forename: Yong Jin
authors.initials: YJ
authors.lastname: Kim
authors.forename: Seok Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Young Min
authors.initials: YM
authors.lastname: Choi
authors.forename: Jung Gu
authors.initials: JG
authors.lastname: Kim
authors.forename: Shin Yong
authors.initials: SY
authors.lastname: Moon
mesh_terms: D000818:Animals; D017077:Culture Media, Conditioned / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D005786:Gene Expression Regulation; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D059471:In Vitro Oocyte Maturation Techniques / Q000379:methods*; D007986:Luteinizing Hormone / Q000494:pharmacology*; D008596:Menotropins / Q000494:pharmacology* / Q000652:urine; D051379:Mice; D008810:Mice, Inbred C57BL; D006080:Ovarian Follicle / Q000187:drug effects* / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D046509:Tissue Culture Techniques
chemical_list: D017077:Culture Media, Conditioned; D011994:Recombinant Proteins; D004958:Estradiol; D008596:Menotropins; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone