title: Structural characterization of anti-UV components from Sasa senanensis Rehder extract.
abstract: BACKGROUND
  Previous studies have shown antiviral, antibacterial and anti-inflammatory activity of alkaline extract of the leaves of Sasa senanensis Rehder (SE). However, active components have not been identified. We isolated the substances that exhibit anti-UV activity and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity from SE and estimated their putative structures.
  
  
  METHODS
  The anti-UV substances (SEE-1 and SEE-2) were isolated from SE by ethanolic extraction, Wakosil chromatography and recycled high-performance liquid chromatography (HPLC) at a yield of 0.22 and 0.18%, respectively. The structural analysis was carried out with (1)H-nuclear magnetic resonance (NMR), (13)C-NMR and UV absorption.
  
  
  RESULTS
  SEE-1 exhibited approximately four-fold higher anti-UV activity and slightly lower DPPH radical-scavenging activity, compared to SE. SEE-1 was identified as p-coumaric acid derivative(s), a lignin precursor.
  
  
  CONCLUSIONS
  The present study demonstrated for the first time the presence of lignin precursors in SE, which may explain why SE exhibits many of the properties of lignin-carbohydrate complexes.
authors.affiliation: Meikai Pharmaco-Medical Laboratory, Meikai University School of Dentistry, Sakado, Saitama 350-0283, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Matsuta
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sakagami
authors.forename: Tomoko
authors.initials: T
authors.lastname: Sugiura
authors.forename: Madoka
authors.initials: M
authors.lastname: Kitajima
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Oizumi
authors.forename: Takaaki
authors.initials: T
authors.lastname: Oizumi
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008031:Lignin / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010084:Oxidation-Reduction / Q000187:drug effects; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D011422:Propionates; D011837:Radiation-Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D031787:Sasa / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D003373:Coumaric Acids; D016166:Free Radical Scavengers; D010851:Picrates; D010936:Plant Extracts; D011422:Propionates; D011837:Radiation-Protective Agents; D000431:Ethanol; D008031:Lignin; C004931:1,1-diphenyl-2-picrylhydrazyl; C495469:p-coumaric acid