title: The genetically modified polysialylated form of neural cell adhesion molecule-positive cells for potential treatment of X-linked adrenoleukodystrophy.
abstract: OBJECTIVE
  Cell transplantation of myelin-producing exogenous cells is being extensively explored as a means of remyelinating axons in X-linked adrenoleukodystrophy. We determined whether 3,3',5-Triiodo-L-thyronine (T3) overexpresses the ABCD2 gene in the polysialylated (PSA) form of neural cell adhesion molecule (NCAM)-positive cells and promotes cell proliferation and favors oligodendrocyte lineage differentiation.
  
  
  METHODS
  PSA-NCAM+ cells from newborn Sprague-Dawley rats were grown for five days on uncoated dishes in defined medium with or without supplementation of basic fibroblast growth factor (bFGF) and/or T3. Then, PSA-NCAM+ spheres were prepared in single cells and transferred to polyornithine/fibronectin-coated glass coverslips for five days to determine the fate of the cells according to the supplementation of these molecules. T3 responsiveness of ABCD2 was analyzed using real-time quantitative polymerase chain reaction, the growth and fate of cells were determined using 5-bromo-2-deoxyuridine incorporation and immunocytochemistry, respectively.
  
  
  RESULTS
  Results demonstrated that T3 induces overexpression of the ABCD2 gene in PSA-NCAM+ cells, and can enhance PSA-NCAM+ cell growth in the presence of bFGF, favoring an oligodendrocyte fate.
  
  
  CONCLUSIONS
  These results may provide new insights into investigation of PSA-NCAM+ cells for therapeutic application to X-linked adrenoleukodystrophy.
authors.affiliation: Department of Physiology, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Jiho
authors.initials: J
authors.lastname: Jang
authors.forename: Han-Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Joon Won
authors.initials: JW
authors.lastname: Kang
authors.forename: Hoon-Chul
authors.initials: HC
authors.lastname: Kang
mesh_terms: D000074061:ATP Binding Cassette Transporter, Subfamily D; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D000326:Adrenoleukodystrophy / Q000235:genetics / Q000628:therapy*; D000818:Animals; D000831:Animals, Newborn; D001973:Bromodeoxyuridine; D002454:Cell Differentiation; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005353:Fibronectins / Q000378:metabolism; D007150:Immunohistochemistry; D019006:Neural Cell Adhesion Molecules / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D012794:Sialic Acids / Q000378:metabolism; D013234:Stem Cells; D013963:Thyroid Hormones / Q000378:metabolism*; D014284:Triiodothyronine / Q000494:pharmacology
chemical_list: D000074061:ATP Binding Cassette Transporter, Subfamily D; D018528:ATP-Binding Cassette Transporters; C525916:Abcd2 protein, rat; D005353:Fibronectins; D019006:Neural Cell Adhesion Molecules; D012794:Sialic Acids; D013963:Thyroid Hormones; C021319:polysialic acid; D014284:Triiodothyronine; D016222:Fibroblast Growth Factor 2; D001973:Bromodeoxyuridine