title: Preparation, characterization, in vivo and biochemical evaluation of brain targeted Piperine solid lipid nanoparticles in an experimentally induced Alzheimer's disease model.
abstract: BACKGROUND
  Alzheimer is a fast growing disease with imprecise chemical treatments. Increased oxidative stress, decrease in acetylcholine concentration, and appearance of amyloidal proteins are reported in pathology of Alzheimer. Chemical drugs are effective but on the cost of detrimental side effects.
  
  
  OBJECTIVE
  Present research is based on Preparation, characterization, behavioral and biochemical evaluation of brain targeted Piperine solid lipid nanoparticles in an experimentally induced Alzheimer's model at a low dose of 2 mg/kg.
  
  
  METHODS
  Piperine solid lipid nanoparticles were prepared by Emulsification-Solvent Diffusion technique with polysorbate-80 coating to impart Brain specific targeting. Experimental Ibotenic acid induced Alzheimer's, Force swimming test, superoxide dismutase, acetylcholenesterase enzymatic assays and also Histopathology of brain cortex was conducted to evaluate the Piperine therapeutic effects in Alzheimer's Disease.
  
  
  RESULTS
  Piperine in solid lipid nanoformulation (2 mg/kg equivalent) reduced the SOD values by 504 ± 44.24 m units, p < 0.05, increased the acetylcholenesterase values by 29.24 ± 4.29 µg/mg, p < 0.01 and reduced immobility to 41.36 ± 3.53 s, p < 0.001 and has shown superior results than Donepezil (5 mg/kg). Histopathology studies revealed the reduced plaques and tangles.
  
  
  CONCLUSIONS
  P-80-PIP-SLN has shown therapeutic effects in Alzheimer via reducing the oxidative stress and reducing the cholinergic degradation at 2 mg/kg dose equivalent.
authors.affiliation: a Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Hamdard University , New Delhi, India.|b Department of Medicinal Chemistry, College of Pharmacy, Qassim University , Qassim, KSA.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Yusuf
authors.affiliation: c Department of Pharmacognosy & Phytochemistry, Faculty of Pharmacy, Hamdard University , New Delhi, India.
authors.forename: Maria
authors.initials: M
authors.lastname: Khan
authors.affiliation: b Department of Medicinal Chemistry, College of Pharmacy, Qassim University , Qassim, KSA.
authors.forename: Riaz A
authors.initials: RA
authors.lastname: Khan
authors.affiliation: a Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Hamdard University , New Delhi, India.
authors.forename: Bahar
authors.initials: B
authors.lastname: Ahmed