title: [Study the rudimentary immunoregulatory mechanisms of Ganoderma Spore oil on immunocompromized mice].
abstract: OBJECTIVE
  To study the rudimentary immunoregulatory mechanisms of Ganoderma spore oil on immunocompromized mice model.
  
  
  METHODS
  Thrity KM mice were randomly selected and assigned into three groups (ten animals per group): the model control group, Ganoderma Lucidum spores oil group and the normal control group. The model control group and Ganoderma Lucidum spores oil group were injected intraperitoneally with cyclophosphamide at 40 mg x kg(-1) d to generate a immunocompromized mice model. The normal control group were administered with 0.9% NaCl solution 0.1 ml/10 g BW as placebo. All agents were given orally once a day, given for consecutive 30 days, Ganoderma Lucidum spores oil group 150 mg/kg, the others given maize 0.1 ml/10 g BW. The serum TNF-alpha , IFN-gamma content of the mice through ELISA kit and the expression levels of IL-2, IL-10, IL-12, IL-4, IFN-gamma, TNF-alpha mRNA in mouse spleen and thymus were examined by RT-PCR to rudimentary study its immunoregulatory mechanisms.
  
  
  RESULTS
  Ganoderma spore oil can significantly increased the content of TNF-alpha and IFN-gamma in the serum and the expression levels of IL-2, IL-10, IL-12, IL-4, IFN-gamma, TNF-alpha mRNA in spleen and thymus, with obvious difference from the model control (P < or = 0.05).
  
  
  CONCLUSIONS
  Ganoderma spore oil can be able to improve the above cytokine ion expression to immunoregulate the immunocompromized mice.
authors.affiliation: Food Science and Technology Institute of Hunan Agricultural University/Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop/Hunan Food Science and Biotechnology Key-Lab, Changsha 410128, China. yiyoujin@163.com
authors.forename: Youjin
authors.initials: Y
authors.lastname: Yi
authors.forename: Shun
authors.initials: S
authors.lastname: Hu
authors.forename: Xingyao
authors.initials: X
authors.lastname: Xiong
authors.forename: Dongbo
authors.initials: D
authors.lastname: Liu
authors.forename: Yingli
authors.initials: Y
authors.lastname: Zhong
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D003520:Cyclophosphamide; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D032902:Ganoderma / Q000737:chemistry*; D016867:Immunocompromised Host / Q000276:immunology*; D008297:Male; D051379:Mice; D009821:Oils / Q000302:isolation & purification / Q000494:pharmacology*; D020940:Reishi; D013172:Spores, Fungal / Q000737:chemistry
chemical_list: D000276:Adjuvants, Immunologic; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D009821:Oils; D003520:Cyclophosphamide