title: Structural studies of an immunostimulating gluco-arabinan from seeds of Caesalpinia bonduc.
abstract: A water-soluble gluco-arabinan (PS-II, M(W)∼62 kDa) isolated from the alkaline extract of the endosperm of Caesalpinia bonduc showed the presence of T-Glcp, (1→4)-Glcp, (1→2,3)-Glcp, T-Araf, (1→5)-Araf, (1→2,5)-Araf, and (1→2,3,5)-Araf in a relative proportion of approximately 2:2:2:3:2:1:1. The proposed repeating unit of the polysaccharide possessed a branched backbone of two (1→3)-α-D-glucopyranose followed by four (1→5)-α-L-arabinofuranose residues. In case of two (1→3)-α-D-glucopyranose, branching occurs at O-2 by a same residue terminated by another one at O-4 position. Out of four (1→5)-α-l-arabinofuranose residues, one residue is terminated at O-2 and O-3 by two arabinofuranose residues and another one situated at the adjacent position is terminated at O-2 with same residue, and two (1→5)-α-L-arabinofuranose residues are free from branching and located before and after the two branched arabinofuranose residues. This gluco-arabinan molecule and previously reported arabinan showed similar extent of splenocytes and thymocytes stimulation, but arabinan showed appreciable macrophage activations.
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Eshita Kar
authors.initials: EK
authors.lastname: Mandal
authors.forename: Soumitra
authors.initials: S
authors.lastname: Mandal
authors.forename: Saikat
authors.initials: S
authors.lastname: Maity
authors.forename: Birendra
authors.initials: B
authors.lastname: Behera
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Maiti
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
mesh_terms: D000818:Animals; D001089:Arabinose / Q000031:analogs & derivatives / Q000737:chemistry; D029911:Caesalpinia / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D016882:ISCOMs / Q000008:administration & dosage / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D009005:Monosaccharides / Q000737:chemistry / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry; D011134:Polysaccharides / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification; D012639:Seeds / Q000737:chemistry*; D059010:Single-Cell Analysis; D060168:Thymocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology
chemical_list: D016882:ISCOMs; D009005:Monosaccharides; D010936:Plant Extracts; D011134:Polysaccharides; C431714:arabinofuranose; C030080:araban; D001089:Arabinose