title: A peptide vaccine based on a B-cell epitope on the VP1 protein of enterovirus 70 induces a strong antibody response.
abstract: Enterovirus 70 (EV70) is the causative agent of acute hemorrhagic conjunctivitis (AHC), for which no effective vaccine is available. This study revealed a high reactivity of the N-terminal region of EV70 VP1 (VP1-1) with an anti-EV70 mouse serum. The analysis of overlapping synthetic peptides of VP1-1 identified a B-cell epitope in this region. The E-peptide (14-ANTVESEIKAELGVI-28) showing the highest reactivity with the anti-EV70 serum induced neutralizing antibodies in mice and reduced the virus titer in the eyes, suggesting that it is a candidate vaccine against AHC caused by EV70.
authors.affiliation: The Catholic University of Korea, Gyeonggi-do, Korea.
authors.forename: Kee-Bum
authors.initials: KB
authors.lastname: Park
authors.forename: Byung-Kwan
authors.initials: BK
authors.lastname: Lim
authors.forename: Michael B
authors.initials: MB
authors.lastname: Ye
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Chung
authors.forename: Jae-Hwan
authors.initials: JH
authors.lastname: Nam
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000917:Antibody Formation; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D003232:Conjunctivitis, Acute Hemorrhagic / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D030016:Enterovirus D, Human / Q000235:genetics / Q000276:immunology*; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000235:genetics / Q000276:immunology*; D022223:Vaccines, Subunit / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D018985:Epitopes, B-Lymphocyte; D010455:Peptides; D022223:Vaccines, Subunit