title: Characterization of yolk sac  proteins of Bos indicus cattle embryos.
abstract: The  yolk sac is an embryonic membrane that is essential for the embryo's initial  survival in many mammals. It also plays an important role in the production of  proteins necessary for development. We studied proteins of the yolk sac in  bovine embryos at up to 40 days of gestation. We examined the yolk sac of 17  bovine embryos at different gestational periods, measuring α-fetoprotein, α-1-antitrypsin,  and transferrin. This experiment was carried out by Western blot technique,  associated with electrophoresis on a 6% sodium dodecyl sulfate polyacrylamide  gel. Mouse monoclonal antibody anti-human-α-fetoprotein, mouse antibody anti-human-transferrin  and rabbit polyclonal anti-human-α-1-antitrypsin were used as primary antibodies, and  conjugated peroxidase as a secondary antibody. We detected the three proteins  in some of the yolk sac samples; however, the bands in some specimens (samples)  were weak, maybe a result of poor antigen-antibody reaction, since the antibodies  used in this study were not specific to bovine proteins. The fact that weak  bands appeared might be due to a weak cross-reaction.
authors.affiliation: Laboratório de Anatomia de Animais Domésticos e Silvestres, Departamento de Cirurgia, Faculdade de Medicina Veterinária e Saúde Animal, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: F S
authors.initials: FS
authors.lastname: Matsumoto
authors.forename: V C
authors.initials: VC
authors.lastname: Oliveira
authors.forename: C A F
authors.initials: CA
authors.lastname: Mançanares
authors.forename: C E
authors.initials: CE
authors.lastname: Ambrosio
authors.forename: M A
authors.initials: MA
authors.lastname: Miglino
mesh_terms: D000818:Animals; D002417:Cattle; D004527:Egg Proteins / Q000378:metabolism*; D055664:Electrochemical Techniques; D005260:Female; D006801:Humans; D008163:Luminescent Measurements; D008970:Molecular Weight; D015017:Yolk Sac / Q000196:embryology* / Q000378:metabolism*
chemical_list: D004527:Egg Proteins