title: Dioscin's antiviral effect in vitro.
abstract: Dioscin is chemical compound obtained from an extract from a medical plant, air potato that is a yam species. Its potential antiviral properties were analyzed in this study. In this study, dioscin's antiviral effects were tested against several viruses including adenovirus, vesicular stomatitis virus (VSV) and hepatitis B virus (HBV). By time-of-addition assay, dioscin not only blocked the initial stage of adenovirus infection, but also affected the host cell's response for viral infection. In addition, 293 cells treated with dioscin displayed decreased mRNA levels for adenovirus receptor (CAR). Over expression of CAR in 293 cells pretreated with dioscin restored the infectivity of adenovirus. The inhibitory effect of dioscin against VSV infection was observed only in 293 cells pretreated with dioscin prior to infection. Finally, dioscin's inhibitory effect on secretion of HBeAg and HBsAg in HBV positive cell line HepG2 2.215 was observed by ELISA assay.
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, China. cliu1234@umd.edu
authors.forename: Chaohong
authors.initials: C
authors.lastname: Liu
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Chunchen
authors.initials: C
authors.lastname: Wu
authors.forename: Rongjuan
authors.initials: R
authors.lastname: Pei
authors.forename: Jianhua
authors.initials: J
authors.lastname: Song
authors.forename: Shiyun
authors.initials: S
authors.lastname: Chen
authors.forename: Xinwen
authors.initials: X
authors.lastname: Chen
mesh_terms: D000256:Adenoviridae / Q000187:drug effects* / Q000502:physiology; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D027742:Dioscorea / Q000737:chemistry; D004144:Diosgenin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D006515:Hepatitis B virus / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018116:Vesiculovirus / Q000187:drug effects* / Q000502:physiology; D053586:Virus Internalization / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D010936:Plant Extracts; C019357:dioscin; D004144:Diosgenin