title: A linker peptide with high affinity towards silica-containing materials.
abstract: A peptide sequence with affinity to silica-containing materials was fused to a truncated form of Streptococcus strain G148 Protein G. The resulting recombinant Linker-Protein G (LPG) was produced in Escherichia coli and purified to apparent homogeneity. It displayed high affinity towards two natural clinoptilolite zeolites. The LPG also displayed high binding affinity towards commercial-grade synthetic zeolite, silica and silica-containing materials. A commercial sample of the truncated Protein G and a basic protein, both without the linker, did not bind to natural or synthetic zeolites or silica. We conclude that the zeolite-binding affinity is mediated by the linker peptide sequence. As a consequence, these data may imply that the binding affinity is directed to the SiO2 component rather than to the atomic orientation on the zeolite crystal surface as previously assumed.
authors.affiliation: Department of Chemistry and Biomolecular Sciences, and Environmental Biotechnology CRC, Macquarie University, Sydney, Australia. anwar.sunna@mq.edu.au
authors.forename: Anwar
authors.initials: A
authors.lastname: Sunna
authors.forename: Fei
authors.initials: F
authors.lastname: Chi
authors.forename: Peter L
authors.initials: PL
authors.lastname: Bergquist
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D055767:Immobilized Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D010455:Peptides / Q000737:chemistry* / Q000235:genetics; D011485:Protein Binding / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D012822:Silicon Dioxide / Q000737:chemistry*; D013291:Streptococcus / Q000737:chemistry* / Q000235:genetics; D017641:Zeolites / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; C034284:IgG Fc-binding protein, Streptococcus; D055767:Immobilized Proteins; D010455:Peptides; D011994:Recombinant Proteins; D017641:Zeolites; D012822:Silicon Dioxide