title: Patterned polymer nanowire arrays as an effective protein immobilizer for biosensing and HIV detection.
abstract: We report an array of polymeric nanowires for effectively immobilizing biomolecules on biochips owing to the large surface area. The nanowires were fabricated in predesigned patterns using an inductively coupled plasma (ICP) etching process. Microfluidic biochips integrated using the substrates with arrays of nanowires and polydimethylsiloxane channels have been demonstrated to be effective for detecting antigens, and a detection limit of antigens at 0.2 μg mL(-1) has been achieved, which is improved by a factor of 50 compared to that based on flat substrates without the nanowires. In addition, the high sensitivity for clinical detection of human immunodeficiency virus (HIV) antibody has also been demonstrated, showing a 20 times enhancement in fluorescent signal intensity between the samples with positive and negative HIV.
authors.affiliation: School of Material Science and Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.
authors.forename: Yue
authors.initials: Y
authors.lastname: Shen
authors.forename: Yingyi
authors.initials: Y
authors.lastname: Liu
authors.forename: Guang
authors.initials: G
authors.lastname: Zhu
authors.forename: Hao
authors.initials: H
authors.lastname: Fang
authors.forename: Yunhui
authors.initials: Y
authors.lastname: Huang
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
authors.forename: Zhong L
authors.initials: ZL
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D000941:Antigens / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D015658:HIV Infections / Q000175:diagnosis*; D006801:Humans; D055767:Immobilized Proteins; D007118:Immunoassay / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D036103:Nanotechnology / Q000379:methods; D053770:Nanowires / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000941:Antigens; D004129:Dimethylpolysiloxanes; D055767:Immobilized Proteins; D011108:Polymers; C013830:baysilon