title: Effect of serum from sub-healthy subjects with fatigue on the gene expression             profile of skeletal muscle cells.
abstract: To investigate the effect of serum from sub-healthy subjects with fatigue on the gene expression profile of skeletal muscle cells (HSkMCs), HSkMCs were cultured in vitro and treated with serum from healthy and sub-healthy subjects independently. Total RNA was extracted, purified and used for reverse transcription into cDNA. This was labeled with one of two fluorescence dyes, Cy3 or Cy5, and hybridized with a Human Genome Array carrying 8064 target genes. The differentially expressed genes were selected with the aid of software. There were 86 differentially expressed genes identified between the sub-healthy subjects and the healthy individuals, of which 60 were upregulated and 26 were downregulated compared with those of the healthy individuals. These genes were mainly associated with the cell cycle, membrane channels, protein transport, energy metabolism and apoptosis. Our results suggest that serum from sub-healthy subjects with fatigue may change the gene expression profile of HSkMCs.
authors.affiliation: Department of Digestive Diseases, Changzhou Hospital of TCM,                     Changzhou 213003, P.R. China.
authors.forename: Bao-Liang
authors.initials: BL
authors.lastname: Li
authors.forename: Xiao-Shan
authors.initials: XS
authors.lastname: Zhao
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Sun
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Ren
authors.initials: R
authors.lastname: Luo
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000328:Adult; D005221:Fatigue / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D018482:Muscle, Skeletal / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism; D059467:Transcriptome; D015854:Up-Regulation
chemical_list: D012313:RNA