title: A convenient photosynthesis of uniformly [¹⁴C]-labelled D-glucose, D-fructose and sucrose, and chemical synthesis of methyl-α-D-glucopyranoside ([U-¹⁴C]-glucose).
abstract: This paper describes a convenient procedure for the radiochemical preparation of d-[U-(14)C]-glucose, d-[U-(14)C]-fructose and [U-(14)C]-sucrose with high specific activity by photosynthesis using 'canna indica' leaf, [(14)C]-carbon dioxide and water in presence of light in a closed system. The [(14)C]-sugars formed were extracted, separated and then purified by paper chromatography. Further, the pure d-[U-(14)C]-glucose obtained was converted to methyl-α-d-glucopyranoside ([U-(14)C]-glucose) by glycosidation with methanol using (i) HCl, the conventional Fischer method (ii) heterogeneous organic cation exchange resin (Amberlite IR-120 (H(+))) and (iii) heterogeneous inorganic cation exchanged montmorillonites called metal M(+n)-monts. The results indicated that the latter in the form of Fe(+3)-montmorillonite gave a better yield ( 65%) as compared to others (40-56%). The radiochemical purity of the no-carrier added product was more than 98%. The product retained its specific activity as that of the starting material which is in the range of 250-300 mCi/mmole (9.25-11.1 GBq/mmole), suitable for use as a radiotracer in biochemical investigations.
authors.affiliation: Radio labelling laboratory, Ogene Systems (I) Pvt. Ltd, #11-6-56, GSR, Estates, Balanagar, Hyderabad, Andhra Pradesh 500037, India. unnyvkp@gmail.com
authors.forename: G
authors.initials: G
authors.lastname: Srinivas
authors.forename: V K P
authors.initials: VK
authors.lastname: Unny
authors.forename: K
authors.initials: K
authors.lastname: Mukkanti
authors.forename: B M
authors.initials: BM
authors.lastname: Choudary