title: Genetic diversity of avian paramyxovirus type 4 isolates from wild ducks in Korea from 2006 to 2011.
abstract: Thirteen isolates of avian paramyxovirus type 4 (APMV-4) isolated from wild ducks in Korea from 2006 to 2011 were genetically characterized by sequence analysis of the N-terminal region of the APMV-4 fusion (F) protein gene. The results revealed that the amino acid sequence homology within Korean isolates was 97.5 % or greater. The homologies of the Korean isolates with the APMV-4/duck/HK/D3/75 and APMV-4/duck/BE/15129/07 strains were 86.9-88.0 and 95.5-96.1 %, respectively. All Korean isolates had sequence motifs of (116)DIQPR↓F(121) at the F0 cleavage site. Phylogenetic analysis based on the N-terminal region of the F protein gene of APMV-4 isolates revealed that all 2006-2011 Korean isolates formed a single genotypic cluster that was phylogenetically different from APMV-4/duck/HK/D3/75 or APMV-4/duck/BE/15129/07 strains. Korean APMV-4 isolates were more closely related to APMV-4/goose/ZA/N1468/10 (isolated in South Africa) than to the Belgium APMV-4 virus. Korean APMV-4 isolates were further divided into at least two subgroups (A and B) based on phylogenetic analysis. Subgroup A viruses were isolated throughout Korea, whereas subgroup B viruses were detected only in isolates from Cheju island in 2011, suggesting that Korean APMV-4 exhibits marked genetic diversity and differs from viruses currently circulating in Europe and other locations.
authors.affiliation: Avian Disease Division, Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang, Gyeonggi 430-757, Republic of Korea.
authors.forename: Kang-Seuk
authors.initials: KS
authors.lastname: Choi
authors.forename: Ji-Ye
authors.initials: JY
authors.lastname: Kim
authors.forename: Soo-Jeong
authors.initials: SJ
authors.lastname: Kye
authors.forename: Choi-Kyu
authors.initials: CK
authors.lastname: Park
authors.forename: Haan-Woo
authors.initials: HW
authors.lastname: Sung
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000835:Animals, Wild / Q000821:virology*; D018109:Avulavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D045463:Avulavirus Infections / Q000662:veterinary* / Q000821:virology; D001715:Bird Diseases / Q000821:virology*; D004372:Ducks / Q000821:virology*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D056910:Republic of Korea; D017386:Sequence Homology, Amino Acid; D014760:Viral Fusion Proteins / Q000235:genetics
chemical_list: D014760:Viral Fusion Proteins