title: Culture on a 3,4-dihydroxy-l-phenylalanine-coated surface promotes the osteogenic differentiation of human mesenchymal stem cells.
abstract: The culture surface can affect the in vitro differentiation of stem cells. In this study, we investigated whether modifying the culture surface with 3,4-dihydroxy-l-phenylalanine (DOPA), an element of mussel adhesion protein, could enhance the in vitro osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMMSCs). hBMMSCs cultured on DOPA-coated plates exhibited better cell adhesion and spreading compared with noncoated conventional tissue culture plates. The DOPA coated did not affect the apoptosis or viability of the cultured hBMMSCs. Also, hBMMSCs cultured on DOPA-coated plates exhibited a higher degree of osteogenic differentiation than did hBMMSCs cultured on noncoated plates, as evaluated with alkaline phosphate (ALP) activity, calcium content, and the mRNA expression of runt-related transcription factor 2, ALP, and osteocalcin. Further, hBMMSCs cultured on DOPA-coated plates demonstrated a higher capability of ectopic bone formation in vivo following implantation in the subcutaneous space of athymic mice compared with hBMMSCs cultured on noncoated plates, as evaluated with microcomputer topography analysis and histomorphometry. These results indicate that modifying the culture surface with DOPA can enhance the in vitro osteogenic differentiation of hBMMSCs.
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Republic of Korea.
authors.forename: Wan-Geun
authors.initials: WG
authors.lastname: La
authors.forename: Jung-Youn
authors.initials: JY
authors.lastname: Shin
authors.forename: Suk Ho
authors.initials: SH
authors.lastname: Bhang
authors.forename: Min
authors.initials: M
authors.lastname: Jin
authors.forename: Hee Hun
authors.initials: HH
authors.lastname: Yoon
authors.forename: Seong-Seo
authors.initials: SS
authors.lastname: Noh
authors.forename: Gun-Il
authors.initials: GI
authors.lastname: Im
authors.forename: Chang-Sung
authors.initials: CS
authors.lastname: Kim
authors.forename: Byung-Soo
authors.initials: BS
authors.lastname: Kim
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology; D004295:Dihydroxyphenylalanine / Q000737:chemistry*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*
chemical_list: D020099:Coated Materials, Biocompatible; D015675:Osteocalcin; D004295:Dihydroxyphenylalanine; D000469:Alkaline Phosphatase