title: Synthesis and characterization of novel polar-embedded silica stationary phases for use in reversed-phase high-performance liquid chromatography.
abstract: We have developed a series of new C(10) dipeptide stationary phases via a simple and effective synthetic method. The preparation of the new phases involves the synthesis of silanes and the surface modification of silica. Chromatographic evaluations of these columns were performed using the Engelhardt, Tanaka, and Neue test mixtures. The applicability of these new stationary phases was also evaluated using a series of diagnostic probes including acids, bases or neutral compounds and several generic applications. These new C(10) dipeptide stationary phases showed excellent hydrolytic stability over a wide pH range. Like other existing amide-embedded columns, these new stationary phases exhibit higher retention for polar and hydrophilic compounds and different selectivity as compared to conventional C(18) columns. These new phases are compatible with 100% aqueous mobile phases, and also provide high column efficiency and good peak shapes for both acidic and basic compounds.
authors.affiliation: Department of Chemistry, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Xiuling
authors.initials: X
authors.lastname: Tang
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Jia
authors.forename: Guangqing
authors.initials: G
authors.lastname: Li
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Sun
authors.forename: Aidong
authors.initials: A
authors.lastname: Wen
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation* / Q000379:methods; D004151:Dipeptides / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D017646:Organosilicon Compounds / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry
chemical_list: D004151:Dipeptides; D017646:Organosilicon Compounds; D004364:Pharmaceutical Preparations