title: Identification of fitness determinants in Enterococcus faecalis by differential proteomics.
abstract: Enterococcus (E.) faecalis is found as commensal in healthy humans, in a variety of fermented foods. It can serve as probiotic but also as pathogen causing endocarditis, bacteremia and urinary tract infections. We have employed a proteomic study with E. faecalis strain OG1RF under different growth conditions and in contact to mouse intestinal cells to identify novel latent and adaptive fitness determinants. These relate to changes in catabolic pathways (BudA), protein biosynthesis (AsnS), cellular surface biosynthesis (RmlA) and regulatory mechanisms (OmpR). This knowledge can be used to derive novel evidence-based targets, which can be used to further elucidate gene expression changes enhancing pathogenicity or fitness in a commensal strain and possibly delineate this species into groups of higher and lower risk for applications in a food or a medical context versus improved treatment strategies of the so far hard to cure diseases.
authors.affiliation: Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Weihenstephaner Steig 16, 85350 Freising, Germany.
authors.forename: Angela G
authors.initials: AG
authors.lastname: Lindenstrauss
authors.forename: Jürgen
authors.initials: J
authors.lastname: Behr
authors.forename: Matthias A
authors.initials: MA
authors.lastname: Ehrmann
authors.forename: Dirk
authors.initials: D
authors.lastname: Haller
authors.forename: Rudi F
authors.initials: RF
authors.lastname: Vogel
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D002460:Cell Line; D003106:Colon / Q000166:cytology; D013293:Enterococcus faecalis / Q000235:genetics* / Q000254:growth & development*; D004847:Epithelial Cells / Q000382:microbiology*; D056084:Genetic Fitness; D051379:Mice; D010100:Oxygen / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D040901:Proteomics
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D010100:Oxygen