title: [Establishment of the human papillomavirus type 31 positive cervical cancer cell line].
abstract: The establishment of in vitro model will provide optimal conditions for the study of human papillomavirus (HPV)-associated cervical cancer. In this study, E6 and E7 gens of HPV31 were cloned and expressed in E. coli. The recombinant proteins were purified and used as antigens to immunize mice for the production of polyclonal antibody. Mammalian expression plasmid pBudCE4. 1-HPV31-E6/E7 was also constructed and transfected into C33A cells. The transfected cells were then selected by Zeocin. The expressions of the E6 and E7 mRNAs and proteins were detected by RT-PCR and Western blot respectively. A stable cervical cancer cell line was established as an in vitro model for the study of human papillomavirus type 31(HPV31) associated cervical cancer.
authors.affiliation: Shenzhen University, Biotechnology Research Center, Shenzhen 518057, China.
authors.forename: Jun-Bo
authors.initials: JB
authors.lastname: Yi
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Mai
authors.forename: Hai-Rong
authors.initials: HR
authors.lastname: Lu
authors.forename: Gang
authors.initials: G
authors.lastname: Zhang
authors.forename: Zhao-Ping
authors.initials: ZP
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002460:Cell Line / Q000821:virology*; D005260:Female; D058952:Human papillomavirus 31 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism; D050725:Papillomavirus E7 Proteins / Q000235:genetics / Q000378:metabolism; D030361:Papillomavirus Infections / Q000821:virology*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: C572886:E6 protein, Human papillomavirus type 31; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D011994:Recombinant Proteins