title: Aspergillus fumigatus activates thrombocytes by secretion of soluble compounds.
abstract: During invasive aspergillosis, platelets might be involved in immune defense, but they also might contribute to the pathology of the disease. We tested the hypothesis that Aspergillus secretes factors that influence the activity and functionality of thrombocytes. Platelets were incubated with medium wherein Aspergillus fumigatus was grown. This fungal culture supernatant potently stimulated thrombocytes in a time- and dose-dependent fashion, inducing release of alpha and dense granules, membrane alterations, aggregation, and formation of microparticles. Fungus-induced platelet activation could be confirmed in vivo: thrombocytes from mice infected with A. fumigatus showed a higher activation level than platelets from noninfected animals. Two stimulating components in the fungal culture supernatant were identified: a fungal serine protease and the mycotoxin gliotoxin. Activation of platelets by fungal factors stimulates antifungal functions: platelets gain the capacity to interact with foreign particles, and they become able to inhibit fungal growth, thus supporting the host immune network. However, some consequences of platelet activation might also be harmful, including excessive inflammation and induction of thrombosis. These findings imply that measuring platelet activation in patients might be an interesting diagnostic parameter.
authors.affiliation: Division of Hygiene and Medical Microbiology, Innsbruck Medical University, Innsbruck, Austria. cornelia.speth@i-med.ac.at
authors.forename: Cornelia
authors.initials: C
authors.lastname: Speth
authors.forename: Magdalena
authors.initials: M
authors.lastname: Hagleitner
authors.forename: Helmut W
authors.initials: HW
authors.lastname: Ott
authors.forename: Reinhard
authors.initials: R
authors.lastname: Würzner
authors.forename: Cornelia
authors.initials: C
authors.lastname: Lass-Flörl
authors.forename: Günter
authors.initials: G
authors.lastname: Rambach
mesh_terms: D000818:Animals; D001228:Aspergillosis / Q000473:pathology; D001232:Aspergillus fumigatus / Q000254:growth & development / Q000276:immunology / Q000378:metabolism* / Q000472:pathogenicity*; D001792:Blood Platelets / Q000187:drug effects* / Q000276:immunology; D017077:Culture Media, Conditioned; D004195:Disease Models, Animal; D005260:Female; D005912:Gliotoxin / Q000378:metabolism; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010974:Platelet Aggregation / Q000187:drug effects*; D057057:Serine Proteases / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D005912:Gliotoxin; D057057:Serine Proteases