title: Optical phantoms with variable properties and geometries for diffuse and fluorescence optical spectroscopy.
abstract: Growing interest in optical instruments for biomedical applications has increased the use of optically calibrated phantoms. Often associated with tissue modeling, phantoms allow the characterization of optical devices for clinical purposes. Fluorescent gel phantoms have been developed, mimicking optical properties of healthy and tumorous brain tissues. Specific geometries of dedicated molds offer multiple-layer phantoms with variable thicknesses and monolayer phantoms with cylindrical inclusions at various depths and diameters. Organic chromophores are added to allow fluorescence spectroscopy. These phantoms are designed to be used with 405 nm as the excitation wavelength. This wavelength is then adapted to excite large endogenous molecules. The benefits of these phantoms in understanding fluorescence tissue analysis are then demonstrated. In particular, detectability aspects as a function of geometrical and optical parameters are presented and discussed.
authors.affiliation: Laboratoire IMNC, UMR 8165, F-91405 Orsay Cedex, France.
authors.forename: Barbara
authors.initials: B
authors.lastname: Leh
authors.forename: Rainer
authors.initials: R
authors.lastname: Siebert
authors.forename: Hussein
authors.initials: H
authors.lastname: Hamzeh
authors.forename: Laurent
authors.initials: L
authors.lastname: Menard
authors.forename: Marie-Alix
authors.initials: MA
authors.lastname: Duval
authors.forename: Yves
authors.initials: Y
authors.lastname: Charon
authors.forename: Darine
authors.initials: D
authors.lastname: Abi Haidar
mesh_terms: D000042:Absorption; D001923:Brain Chemistry; D001932:Brain Neoplasms / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008954:Models, Biological; D061848:Optical Imaging / Q000295:instrumentation* / Q000379:methods*; D019047:Phantoms, Imaging; D012031:Refractometry; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes