title: During embryogenesis, esrp1 expression is restricted to a subset of epithelial cells and is  associated with splicing of a number of developmentally important genes.
abstract: BACKGROUND
  Development of a mature organism from a single cell requires a series of important morphological changes, which is in part regulated by alternative splicing. In this article, we report the expression of Esrp1 during early mouse embryogenesis, a splicing factor implicated in epithelial to mesenchymal transitions.
  
  
  RESULTS
  By qRT-PCR, we find higher expression of Esrp1 and Esrp2 in placenta compared to the embryos. We also find a correlation between the expression of Esrp1 and alternative splicing of several known target exons. Using in situ RNA hybridization we show that while Esrp1 expression is ubiquitous in embryonic day (E)6.5 mouse embryos, expression becomes restricted to the chorion and definitive endoderm starting at E7.5. Esrp1 expression was consistently restricted to a subset of epithelial cell types in developing embryos from E9.5 to E13.5.
  
  
  CONCLUSIONS
  Our results suggest that Esrp1 could play an important role in the morphological changes underlying embryogenesis of the placenta and embryo.
authors.affiliation: Department of Human Genetics, McGill University, Montreal, Quebec, Canada.
authors.forename: Timothée
authors.initials: T
authors.lastname: Revil
authors.forename: Loydie A
authors.initials: LA
authors.lastname: Jerome-Majewska
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D047108:Embryonic Development / Q000502:physiology*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006801:Humans; D051379:Mice; D009928:Organ Specificity / Q000502:physiology; D010920:Placenta / Q000166:cytology / Q000378:metabolism; D011247:Pregnancy; D012326:RNA Splicing / Q000502:physiology*; D016601:RNA-Binding Proteins / Q000096:biosynthesis*
chemical_list: D016601:RNA-Binding Proteins