title: Expression of YB-1 enhances production of murine leukemia virus vectors by stabilizing genomic viral RNA.
abstract: Murine leukemia virus (MLV)-based retroviral vectors is widely used for gene transfer and basic research, and production of high-titer retroviral vectors is very important. Here we report that expression of the Y-box binding protein 1 (YB-1) enhanced the production of infectious MLV vectors. YB-1 specifically increased the stability of viral genomic RNA in virus-producing cells, and thus increasing viral RNA levels in both producer cells and virion particles. The viral element responsive to YB-1 was mapped to the repeat sequence (R region) in MLV genomic RNA. These results identified YB-1 as a MLV mRNA stabilizer, which can be used for improving production of MLV vectors.
authors.affiliation: Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Xinlu
authors.initials: X
authors.lastname: Wang
authors.forename: Guangxia
authors.initials: G
authors.lastname: Gao
mesh_terms: D001483:Base Sequence; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000096:biosynthesis* / Q000235:genetics; D016679:Genome, Viral / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics* / Q000502:physiology; D020871:RNA Stability / Q000235:genetics*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D020506:Untranslated Regions / Q000235:genetics; D014771:Virion / Q000235:genetics / Q000502:physiology; D051840:Y-Box-Binding Protein 1 / Q000235:genetics*
chemical_list: D012367:RNA, Viral; D020506:Untranslated Regions; D051840:Y-Box-Binding Protein 1