title: Assessment of radiation damage in single-shot coherent diffraction of DNA molecules by an extreme-ultraviolet free-electron laser.
abstract: We have investigated the progress of structural distortions in DNA molecules by single-shot coherent diffraction using extreme-ultraviolet radiation from a free-electron laser. A speckle pattern of DNA molecules was successfully acquired using photons in a single pulse with a 100 fs pulse width. The radiation damage was assessed by a cross correlation, revealing that the first exposure has significantly deformed most of the original structures. Molecules were not completely destroyed by the first single-shot exposure and underwent subsequent distortions through continued exposure, until eventually deforming into a radiation-hard structure.
authors.affiliation: RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
authors.forename: Jaehyun
authors.initials: J
authors.lastname: Park
authors.forename: Daewoong
authors.initials: D
authors.lastname: Nam
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Kohmura
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Nagasono
authors.forename: Yongmoon
authors.initials: Y
authors.lastname: Jeon
authors.forename: Jong-Bong
authors.initials: JB
authors.lastname: Lee
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Changyong
authors.initials: C
authors.lastname: Song
mesh_terms: D018360:Crystallography, X-Ray / Q000379:methods; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects*; D004583:Electrons; D007834:Lasers; D008853:Microscopy / Q000379:methods; D016011:Normal Distribution; D055095:Optics and Photonics; D017785:Photons; D011835:Radiation Protection / Q000379:methods; D014466:Ultraviolet Rays
chemical_list: D004247:DNA