title: Induction of osteogenic differentiation of adipose derived stem cells by microstructured nitinol actuator-mediated mechanical stress.
abstract: The development of large tissue engineered bone remains a challenge in vitro, therefore the use of hybrid-implants might offer a bridge between tissue engineering and dense metal or ceramic implants. Especially the combination of the pseudoelastic implant material Nitinol (NiTi) with adipose derived stem cells (ASCs) opens new opportunities, as ASCs are able to differentiate osteogenically and therefore enhance osseointegration of implants. Due to limited knowledge about the effects of NiTi-structures manufactured by selective laser melting (SLM) on ASCs the study started with an evaluation of cytocompatibility followed by the investigation of the use of SLM-generated 3-dimensional NiTi-structures preseeded with ASCs as osteoimplant model. In this study we could demonstrate for the first time that osteogenic differentiation of ASCs can be induced by implant-mediated mechanical stimulation without support of osteogenic cell culture media. By use of an innovative implant design and synthesis via SLM-technique we achieved high rates of vital cells, proper osteogenic differentiation and mechanically loadable NiTi-scaffolds could be achieved.
authors.affiliation: Department of Plastic-, Hand- and Reconstructive Surgery, Hannover Medical School, Hannover, Germany. strauss.sarah@mh-hannover.de
authors.forename: Sarah
authors.initials: S
authors.lastname: Strauß
authors.forename: Sonja
authors.initials: S
authors.lastname: Dudziak
authors.forename: Ronny
authors.initials: R
authors.lastname: Hagemann
authors.forename: Stephan
authors.initials: S
authors.lastname: Barcikowski
authors.forename: Malte
authors.initials: M
authors.lastname: Fliess
authors.forename: Meir
authors.initials: M
authors.lastname: Israelowitz
authors.forename: Dietmar
authors.initials: D
authors.lastname: Kracht
authors.forename: Jörn W
authors.initials: JW
authors.lastname: Kuhbier
authors.forename: Christine
authors.initials: C
authors.lastname: Radtke
authors.forename: Kerstin
authors.initials: K
authors.lastname: Reimers
authors.forename: Peter M
authors.initials: PM
authors.lastname: Vogt
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000497:Alloys / Q000737:chemistry*; D000880:Anthraquinones; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000502:physiology*; D019736:Prostheses and Implants; D013234:Stem Cells / Q000502:physiology*; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D000497:Alloys; D000880:Anthraquinones; C013616:nitinol; C010078:alizarin