title: Establishment of a method to determine the magnetic particles in mouse tissues.
abstract: This work is aimed to evaluate a method to detect the residual magnetic nanoparticles (MNPs) in animal tissues. Ferric ions released from MNPs through acidification with hydrochloric acid can be measured by complexation with potassium thiocyanate. MNPs in saline could be well detected by this chemical colorimetric method, whereas the detected sensitivity decreased significantly when MNPs were mixed with mouse tissue homogenates. In order to check the MNPs in animal tissues accurately, three improvements have been made. Firstly, proteinase K was used to digest the proteins that might bind with iron, and secondly, ferrosoferric oxide (Fe3O4) was collected by a magnetic field which could capture MNPs and leave the bio-iron in the supernatant. Finally, the collected MNPs were carbonized in the muffle furnace at 420°C before acidification to ruin the groups that might bind with ferric ions such as porphyrin. Using this method, MNPs in animal tissues could be well measured while avoiding the disturbance of endogenous iron and iron-binding groups.
authors.affiliation: Beijing Institute of Pharmacoloy and Toxicology, Beijing, 100850, People's Republic of China. wangyuxia1962@hotmail.com.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Wu
authors.forename: Wuxu
authors.initials: W
authors.lastname: Zhang
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Wang
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.forename: Guo
authors.initials: G
authors.lastname: Gao
authors.forename: Na
authors.initials: N
authors.lastname: Dong
authors.forename: Hengyao
authors.initials: H
authors.lastname: Hu
authors.forename: Kan
authors.initials: K
authors.lastname: Wang
authors.forename: Junhua
authors.initials: J
authors.lastname: Wu
authors.forename: Zhongcai
authors.initials: Z
authors.lastname: Gao
authors.forename: Daxiang
authors.initials: D
authors.lastname: Cui