title: Antifouling surfaces for proteins labeled with dye-doped silica nanoparticles.
abstract: We report that proteins labeled with fluorescein-doped silica nanoparticles (FSNPs) showed drastically different fouling behavior than those labeled with the fluorescein dye. Arrays of polymer films were covalently immobilized on silicon wafers and were treated with protein conjugated on FSNPs. Fluorescence imaging showed that the protein-FSNP conjugate adsorbed strongly on hydrophilic polymers such as poly(ethylene oxide) (PEO) and weakly on hydrophobic polymers such as polystyrene (PS), and the extent of adsorption decreased with increasing hydrophobicity of the polymer film. Thus, carbohydrate microarrays probed with FSNP-labeled lectin showed significantly enhanced signals when PS was used as the antifouling coating than when PEO was used, or when using bovine serum albumin as the blocking agent.
authors.affiliation: Department of Chemistry, University of Massachusetts Lowell, 01854, United States.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Qi
authors.initials: Q
authors.lastname: Tong
authors.forename: Mingdi
authors.initials: M
authors.lastname: Yan
mesh_terms: D000327:Adsorption; D002241:Carbohydrates / Q000737:chemistry; D003208:Concanavalin A / Q000737:chemistry / Q000378:metabolism; D019793:Fluorescein / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism; D046228:Microarray Analysis; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D020349:Surface Plasmon Resonance; D013499:Surface Properties
chemical_list: D002241:Carbohydrates; D005456:Fluorescent Dyes; D037102:Lectins; D011137:Polystyrenes; D003208:Concanavalin A; D011092:Polyethylene Glycols; D012822:Silicon Dioxide; D019793:Fluorescein