title: Generation of integration-free neural progenitor cells from cells in human urine.
abstract: Human neural stem cells hold great promise for research and therapy in neural disease. We describe the generation of integration-free and expandable human neural progenitor cells (NPCs). We combined an episomal system to deliver reprogramming factors with a chemically defined culture medium to reprogram epithelial-like cells from human urine into NPCs (hUiNPCs). These transgene-free hUiNPCs can self-renew and can differentiate into multiple functional neuronal subtypes and glial cells in vitro. Although functional in vivo analysis is still needed, we report that the cells survive and differentiate upon transplant into newborn rat brain.
authors.affiliation: Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
authors.forename: Lihui
authors.initials: L
authors.lastname: Wang
authors.forename: Linli
authors.initials: L
authors.lastname: Wang
authors.forename: Wenhao
authors.initials: W
authors.lastname: Huang
authors.forename: Huanxing
authors.initials: H
authors.lastname: Su
authors.forename: Yanting
authors.initials: Y
authors.lastname: Xue
authors.forename: Zhenghui
authors.initials: Z
authors.lastname: Su
authors.forename: Baojian
authors.initials: B
authors.lastname: Liao
authors.forename: Haitao
authors.initials: H
authors.lastname: Wang
authors.forename: Xichen
authors.initials: X
authors.lastname: Bao
authors.forename: Dajiang
authors.initials: D
authors.lastname: Qin
authors.forename: Jufang
authors.initials: J
authors.lastname: He
authors.forename: Wutian
authors.initials: W
authors.lastname: Wu
authors.forename: Kwok Fai
authors.initials: KF
authors.lastname: So
authors.forename: Guangjin
authors.initials: G
authors.lastname: Pan
authors.forename: Duanqing
authors.initials: D
authors.lastname: Pei
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D001921:Brain / Q000166:cytology*; D002454:Cell Differentiation; D065150:Cellular Reprogramming; D004847:Epithelial Cells / Q000166:cytology*; D020869:Gene Expression Profiling; D006801:Humans; D007124:Immunoenzyme Techniques; D058953:Neural Stem Cells / Q000166:cytology* / Q000637:transplantation; D009457:Neuroglia / Q000166:cytology; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D033581:Stem Cell Transplantation; D023822:Tissue Engineering / Q000379:methods*; D014556:Urine / Q000737:chemistry / Q000166:cytology*
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger