title: Effects of extraction condition on structural features and anticoagulant activity of F. vesca L. conjugates.
abstract: From the air-dried Wild strawberry (Fragaria vesca L., family Rosaceae) leaves five water-soluble glycoconjugates Fv I-V by different extraction conditions have been isolated. Effects of extraction steps/agents on chemical composition and anticoagulant activity of Fv I-V were examined. Dark brown F. vesca conjugates Fv I-V were recovered in 4.5-8.4% yields, based on dry herb. Isolates were composed of carbohydrate, phenolic and protein components. Fv I-V displayed on HPLC broad molecule-mass distribution patterns with dominance of low molecule-masses 9-14 kDa. Their carbohydrate parts revealed high hexuronic acids content (35-60%) while the dominant neutral sugars - galactose, arabinose and rhamnose were found in lower amounts and indicated the presence of rhamnogalacturonans associated with arabinogalactans in all F. vesca preparations. In all Fv I-V isolates high polyphenolic contents were determined, whereas proteins were found in low amounts only. In in vitro experiments on human pooled plasma Fv I-V showed at higher concentrations complete inhibition of plasma clot formation and the most active conjugates in aPTT, PT and TT tests were shown to be Fv I and Fv III, containing the highest amounts of phenolics.
authors.affiliation: Regional Specialist Hospital in Wrocław, Research and Development Centre, ul. Kamieńskiego 73a, 51-124 Wrocław, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Pawlaczyk
authors.forename: Marta
authors.initials: M
authors.lastname: Lewik-Tsirigotis
authors.forename: Peter
authors.initials: P
authors.lastname: Capek
authors.forename: Mária
authors.initials: M
authors.lastname: Matulová
authors.forename: Vlasta
authors.initials: V
authors.lastname: Sasinková
authors.forename: Paweł
authors.initials: P
authors.lastname: Dąbrowski
authors.forename: Wojciech
authors.initials: W
authors.lastname: Witkiewicz
authors.forename: Roman
authors.initials: R
authors.lastname: Gancarz
mesh_terms: D000925:Anticoagulants / Q000008:administration & dosage / Q000737:chemistry; D001777:Blood Coagulation / Q000187:drug effects*; D031985:Fragaria / Q000737:chemistry*; D005685:Galactans / Q000737:chemistry / Q000302:isolation & purification; D006001:Glycoconjugates / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification; D006603:Hexuronic Acids / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D000925:Anticoagulants; D005685:Galactans; D006001:Glycoconjugates; D006603:Hexuronic Acids; C005653:arabinogalactan