title: [Screening of two straw-cellulose degrading actinomycetes].
abstract: OBJECTIVE
  The aim of this study was to screen microorganisms that could degrade rice straw.
  
  
  METHODS
  We used selective medium to screen strains and determined straw fracture tension strength, weight loss, lignocellulose decomposition rate and extracellular enzyme activity as re-screening methods after 10 days shake flask culture.
  
  
  RESULTS
  We isolated two antinomycetes (A3 and A6), the highest cellulose enzyme activity of holoenzyme, beta3-Glucosidase, endonuclease and exonclease for A3 were 12.84, 6.23, 24.56 and 14.00 U/mL, and for A6 12.85, 6.53, 17.80 and 18.80 U/mL. The hemicelluloses enzyme activity was 83.05 for A3 and 52.98 U/mL for A6. Both strains belonged to Streptomyces. With 10 days' treatment, inoculated straws showed a decrease of straw fracture tension strength by 62.67% (A3) and 66.67% (A6), while weight loss of straw was 31.50% (A3) and 35.83% (A6). A3's decomposition rate of cellulose, hemicellulose and lignin was 38.73% , 33.16% and 20.68% , and 47.69% , 28.64% and 22.59% for A6.
  
  
  CONCLUSIONS
  Antinomycetes A3 and A6 could degrad cellulose, hemicellulose and lignin.
authors.affiliation: Soil and Fertilizer Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Plant Nutrition and Fertilizer in South Region/Guangdong Key Laboratory of Nutrient Cycling and Farmland Conservation, Guangzhou, 510640 China. irisgu0818@yahoo.com.cn
authors.forename: Wenjie
authors.initials: W
authors.lastname: Gu
authors.forename: Fabao
authors.initials: F
authors.lastname: Zhang
authors.forename: Peizhi
authors.initials: P
authors.lastname: Xu
authors.forename: Kaizhi
authors.initials: K
authors.lastname: Xie
authors.forename: Shuanhu
authors.initials: S
authors.lastname: Tang
mesh_terms: D039903:Actinobacteria / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D002480:Cellulase / Q000235:genetics / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D008969:Molecular Sequence Data; D012275:Oryza / Q000378:metabolism* / Q000382:microbiology; D010802:Phylogeny; D018547:Plant Stems / Q000378:metabolism* / Q000382:microbiology; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D002482:Cellulose; D002480:Cellulase