title: ADAPT-NMR Enhancer: complete package for reduced dimensionality in protein NMR spectroscopy.
abstract: CONCLUSIONS
  ADAPT-nuclear magnetic resonance (ADAPT-NMR) offers an automated approach to the concurrent acquisition and processing of protein NMR data with the goal of complete backbone and side chain assignments. What the approach lacks is a useful graphical interface for reviewing results and for searching for missing peaks that may have prevented assignments or led to incorrect assignments. Because most of the data ADAPT-NMR collects are 2D tilted planes used to find peaks in 3D spectra, it would be helpful to have a tool that reconstructs the 3D spectra. The software package reported here, ADAPT-NMR Enhancer, supports the visualization of both 2D tilted planes and reconstructed 3D peaks on each tilted plane. ADAPT-NMR Enhancer can be used interactively with ADAPT-NMR to automatically assign selected peaks, or it can be used to produce PINE-SPARKY-like graphical dialogs that support atom-by-atom and peak-by-peak assignment strategies. Results can be exported in various formats, including XEASY proton file (.prot), PINE pre-assignment file (.str), PINE probabilistic output file, SPARKY peak list file (.list) and TALOS+ input file (.tab). As an example, we show how ADAPT-NMR Enhancer was used to extend the automated data collection and assignment results for the protein Aedes aegypti sterol carrier protein 2.
  
  
  BACKGROUND
  The program, in the form of binary code along with tutorials and reference manuals, is available at http://pine.nmrfam.wisc.edu/adapt-nmr-enhancer.
authors.affiliation: National Magnetics Resonance Facility at Madison, University of Wisconsin-Madison, Madison, WI 53706, USA. whlee@nmrfam.wisc.edu
authors.forename: Woonghee
authors.initials: W
authors.lastname: Lee
authors.forename: Arash
authors.initials: A
authors.lastname: Bahrami
authors.forename: John L
authors.initials: JL
authors.lastname: Markley
mesh_terms: D000465:Algorithms; D002352:Carrier Proteins / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D012984:Software
chemical_list: D002352:Carrier Proteins; D011506:Proteins; C072087:sterol carrier proteins