title: Preparation and characterization of an imprinted monolith by atom transfer radical polymerization assisted by crowding agents.
abstract: A method based on reverse atom transfer radical polymerization (R-ATRP) and molecular crowding has been used for design and synthesis of monolithic molecularly imprinted polymers (MIPs) capable of recognizing ibuprofen (IBU). 4-Vinylpyridine (4-VP) was used as the functional monomer, and ethylene glycol dimethacrylate (EDMA) was the crosslinking monomer. Azobisisobutyronitrile (AIBN)-CuCl(2)-N,N,N',N",N"-pentamethyldiethylenetriamine (PMDETA) was used as the initiating system. Compared with conventional radical polymerization-based IBU-MIPs, the imprinting effects of the obtained IBU-MIPs was enhanced, suggesting the merit of combination of reverse ATRP and molecular crowding. In addition, it was found that the polymerization time of the molecularly imprinted monolithic column, the amount of template, the degree of crosslinking, and the composition of mobile phase greatly affected retention of the template and the performance of molecular recognition.
authors.affiliation: Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Ban
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.forename: Bang-Li
authors.initials: BL
authors.lastname: Deng
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Huang
authors.forename: Zhao-Sheng
authors.initials: ZS
authors.lastname: Liu
mesh_terms: D000327:Adsorption; D002853:Chromatography, Liquid / Q000379:methods*; D007052:Ibuprofen / Q000737:chemistry* / Q000302:isolation & purification; D054802:Molecular Imprinting; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D011108:Polymers; D011725:Pyridines; C029351:4-vinylpyridine; D007052:Ibuprofen