title: Streptococcus gordonii collagen-binding domain protein CbdA may enhance bacterial survival in instrumented root canals ex vivo.
abstract: BACKGROUND
  The surface-associated collagen-binding protein Ace of Enterococcus faecalis has been implicated as a virulence factor that contributes to bacterial persistence in endodontic infections. The purpose of this study was to determine if proteins with amino acid sequence similarity to Ace found in more abundant oral streptococci could play a similar role in potentially enhancing endodontic infections.
  
  
  METHODS
  A Streptococcus gordonii gene similar to ace was identified by genome sequence searches in silico. An isogenic derivative of strain DL1 with a disruption in the identified gene was constructed by allelic replacement. Parent and mutant strains were characterized for their ability to bind immobilized collagen type 1 in a microtiter plate-binding assay. Survival of the strains in a human tooth ex vivo-instrumented root canal model was compared by inoculating canals with parental or mutant bacteria and determining the colony-forming units (CFUs) recovered at various time points over a 12-day period.
  
  
  RESULTS
  The S. gordonii gene, encoding a protein with a conserved collagen-binding domain similar to that of Ace, was designated cbdA. The cbdA-deficient cells were less able to bind collagen type 1 than parental cells (P < .0001). Genetic complementation of the cbdA-deficient strain restored the collagen-binding phenotype. By day 12, significantly fewer (P = .03) cbdA-deficient than parental CFUs were recovered from instrumented canals.
  
  
  CONCLUSIONS
  A gene encoding a putative collagen-binding protein was identified in S. gordonii. Fewer S. gordonii cbdA-deficient cells survived ex vivo compared with parental cells, suggesting that collagen-binding proteins may contribute to the persistence of oral streptococci in instrumented root canals.
authors.affiliation: Department of Periodontics and Endodontics, University at Buffalo, State University of New York, Buffalo, New York, USA.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Moses
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Power
authors.forename: Amy M
authors.initials: AM
authors.lastname: Jesionowski
authors.forename: Howard F
authors.initials: HF
authors.lastname: Jenkinson
authors.forename: Eugene A
authors.initials: EA
authors.lastname: Pantera
authors.forename: M Margaret
authors.initials: MM
authors.lastname: Vickerman
mesh_terms: D000328:Adult; D001422:Bacterial Adhesion / Q000235:genetics; D058491:Bacterial Load; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D002874:Chromosome Mapping; D024042:Collagen Type I / Q000378:metabolism; D017124:Conserved Sequence / Q000235:genetics; D003786:Dental Pulp Cavity / Q000382:microbiology*; D020868:Gene Silencing; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics; D050296:Microbial Viability / Q000235:genetics; D009154:Mutation / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D010957:Plasmids; D018915:Root Canal Preparation / Q000379:methods*; D054773:Streptococcus gordonii / Q000235:genetics / Q000502:physiology*; D037521:Virulence Factors / Q000235:genetics / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D024042:Collagen Type I; D008565:Membrane Proteins; D037521:Virulence Factors