title: Quantification of IncP-1 plasmid prevalence in environmental samples.
abstract: To study the role of broad-host-range IncP-1 plasmids in bacterial adaptability to irregular environmental challenges, a quantitative real-time PCR assay was developed that specifically detects the korB gene, which is conserved in all IncP-1 plasmids, in environmental samples. IncP-1 plasmid dynamics in a biopurification system for pesticide wastes were analyzed.
authors.affiliation: Julius Kühn-Institut-Federal Research Center for Cultivated Plants, Department of Epidemiology and Pathogen Diagnostics, Braunschweig, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Jechalke
authors.forename: Simone
authors.initials: S
authors.lastname: Dealtry
authors.forename: Kornelia
authors.initials: K
authors.lastname: Smalla
authors.forename: Holger
authors.initials: H
authors.lastname: Heuer
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D041963:Bacteroidetes / Q000235:genetics*; D001711:Biotransformation; D004783:Environmental Microbiology; D010575:Pesticides / Q000378:metabolism; D010957:Plasmids / Q000032:analysis* / Q000145:classification; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D001426:Bacterial Proteins; C053863:KorB protein, Plasmid RK2; D010575:Pesticides