title: Spread of different rhinovirus B genotypes in hospitalized children in Spain.
abstract: Human Rhinovirus (HRV) classification is an evolving process. New genotypes have been described within HRV-A and HRV-C species, but only one has been accepted related to HRV-B. From 2003 to 2010, a total of 3987 nasopharyngeal aspirate samples were taken from pediatric patients admitted to the Severo Ochoa Hospital in Madrid (Spain). After viral analysis, 949 (23.8%) tested positive to HRV. A random selection of 397 (42%) positive samples showed that 39 (9.8%) were HRV-B. The sequencing of partial VP4/VP2 coding region revealed the spread of 13 of 25 defined HRV-B serotypes and three putative new genotypes. Such results remark the high diversity of HRV-B.
authors.affiliation: Influenza and Respiratory Virus Laboratory, National Center for Microbiology, Instituto de Salud Carlos III (ISCIII), 28220 Majadahonda, Madrid, Spain. tcuevas@isciii.es
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Cuevas
authors.forename: Mar
authors.initials: M
authors.lastname: Molinero
authors.forename: Francisco
authors.initials: F
authors.lastname: Pozo
authors.forename: Cristina
authors.initials: C
authors.lastname: Calvo
authors.forename: Maria Luz
authors.initials: ML
authors.lastname: García-García
authors.forename: Noelia
authors.initials: N
authors.lastname: Reyes
authors.forename: Juan
authors.initials: J
authors.lastname: Ledesma
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Casas
mesh_terms: D000293:Adolescent; D002648:Child; D002673:Child, Hospitalized; D002675:Child, Preschool; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000453:epidemiology / Q000821:virology*; D012141:Respiratory Tract Infections / Q000453:epidemiology / Q000821:virology*; D012229:Rhinovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D013030:Spain