title: Inhibition of tumor growth in vitro and in vivo by fucoxanthin against melanoma B16F10 cells.
abstract: The present study was designed to evaluate the molecular mechanisms of fucoxanthin against melanoma cell lines (B16F10 cells). Fucoxanthin reduced the proliferation of B16F10 cells in a dose-dependent manner accompanied by the induction of cell cycle arrest during the G(0)/G(1) phase and apoptosis. Fucoxanthin-induced G(0)/G(1) arrest was associated with a marked decrease in the protein expressions of phosphorylated-Rb (retinoblastoma protein), cyclin D (1 and 2) and cyclin-dependent kinase (CDK) 4 and up-regulation of the protein levels of p15(INK4B) and p27(Kip1). Fucoxanthin-induced apoptosis was accompanied with the down-regulation of the protein levels of Bcl-xL, an inhibitor of apoptosis proteins (IAPs), resulting in a sequential activation of caspase-9, caspase-3, and PARP. Furthermore, the anti-tumor effect of fucoxanthin was assessed in vivo in Balb/c mice. Intraperitoneal administration of fucoxanthin significantly inhibited the growth of tumor mass in B16F10 cells implanted mice.
authors.affiliation: Marine Bio Research Team, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea.
authors.forename: Kil-Nam
authors.initials: KN
authors.lastname: Kim
authors.forename: Ginnae
authors.initials: G
authors.lastname: Ahn
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Heo
authors.forename: Sung-Myung
authors.initials: SM
authors.lastname: Kang
authors.forename: Min-Cheol
authors.initials: MC
authors.lastname: Kang
authors.forename: Hye-Mi
authors.initials: HM
authors.lastname: Yang
authors.forename: Daekyung
authors.initials: D
authors.lastname: Kim
authors.forename: Seong Woon
authors.initials: SW
authors.lastname: Roh
authors.forename: Se-Kwon
authors.initials: SK
authors.lastname: Kim
authors.forename: Byong-Tae
authors.initials: BT
authors.lastname: Jeon
authors.forename: Pyo-Jam
authors.initials: PJ
authors.lastname: Park
authors.forename: Won-Kyo
authors.initials: WK
authors.lastname: Jung
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Jeon
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D018797:Cell Cycle Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D047368:Tumor Burden / Q000187:drug effects; D024341:Xanthophylls / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000970:Antineoplastic Agents; D018797:Cell Cycle Proteins; D024341:Xanthophylls; C025164:fucoxanthin