title: Identification of regulatory elements in the glucoamylase-encoding gene (glaB) promoter from Aspergillus oryzae.
abstract: The Aspergillus oryzae glucoamylase-encoding gene glaB is expressed specifically and strongly only during solid-state cultivation (SSC). To elucidate the basis for the specificity, the glaB promoter was analyzed by electrophoretic gel mobility shift assay (EMSA) which indicated two protein-binding elements from -382 to -353 and from -332 to -313. To confirm that these regions contained cis-elements, deletion analysis of the promoter was undertaken using β-glucuronidase as a reporter. The results of the deletion analysis were consistent with the EMSA results. The promoter missing the -332 to -313 element was not induced by low water activity stress during SSC.
authors.affiliation: Research Institute, Gekkeikan Sake Co. Ltd., 101 Shimotoba-koyanagi-cho, Fushimi-ku, Kyoto, Kyoto 612-8385, Japan. hisada@gekkeikan.co.jp
authors.forename: Hiromoto
authors.initials: H
authors.lastname: Hisada
authors.forename: Motoaki
authors.initials: M
authors.lastname: Sano
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ishida
authors.forename: Yoji
authors.initials: Y
authors.lastname: Hata
authors.forename: Masayuki
authors.initials: M
authors.lastname: Machida
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology* / Q000235:genetics*; D001665:Binding Sites; D004252:DNA Mutational Analysis; D024202:Electrophoretic Mobility Shift Assay; D015966:Gene Expression Regulation, Fungal; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D017384:Sequence Deletion
chemical_list: D005087:Glucan 1,4-alpha-Glucosidase