title: Isolation of the retinal isomers from the isomerization of all-trans-retinal by flash countercurrent chromatography.
abstract: The isolation of the retinal isomers from all-trans-retinal was performed by flash countercurrent chromatography. In each separation, isomerization reaction solution of 200mg all-trans-retinal could be loaded on a 1200 mL of high-speed countercurrent chromatographic column with 5mm bore, eluted by a mobile phase flow rate of 25 mL/min, resulting in 63 mg of 11-cis-retinal, 24 mg of 13-cis-retinal and 26 mg of 9-cis-retinal with purities more than 95%. n-Hexane-acetonitrile (3:1) was used as the solvent system which possesses the advantages of simplicity, re-use of the solvent and multiple injections. This method could be used to prepare 13-cis-retinal, 11-cis-retinal and 9-cis-retinal for the photoisomerization investigation, such as the effect of 11-cis-retinal in the visual system.
authors.affiliation: Institute of Food Chemistry, Zhejiang Gongshang University, Hangzhou 3100125, China.
authors.forename: Minfei
authors.initials: M
authors.lastname: He
authors.forename: Wenkai
authors.initials: W
authors.lastname: Du
authors.forename: Qingbao
authors.initials: Q
authors.lastname: Du
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.forename: Changqian
authors.initials: C
authors.lastname: Ke
authors.forename: Yang
authors.initials: Y
authors.lastname: Ye
authors.forename: Qizhen
authors.initials: Q
authors.lastname: Du
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D007536:Isomerism; D012172:Retinaldehyde / Q000737:chemistry / Q000302:isolation & purification*; D014801:Vitamin A / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D014801:Vitamin A; D012172:Retinaldehyde