title: [Isolation and identification of shellfish toxins from contaminated blue mussel (Mytilus edulis) from the East China Sea].
abstract: OBJECTIVE
  To investigate the contamination of shellfish poisoning of mussels, the poisonous constituents in that were isolated and identified.
  
  
  METHODS
  The mussel tissue homogenate was extracted by acetone, and then the acetone extract was partitioned between diethyl ether and water. The ether extract was fractionated by column chromatography over silica gel and further isolated by semi-preparative RP-HPLC, monitored by ultra performance liquid chromatography coupled with triple quadrupole mass spectrometry.
  
  
  RESULTS
  Four poisonous constituents were isolated. Two of them were elucidated as pectenotoxin-2 seco acid and 7-epi-pectenotoxin-2 seco acid, respectively, on the basis of mass spectral data and compared with the production of enzymatic hydrolysis of PTX-2, and others were identified as okadaic acid and dinophysistoxin-1 by UPLC-MS/MS analysis compared with standard substances.
  
  
  CONCLUSIONS
  OA, DTX-1, 7-epi-PTX-2sa and PTX-2sa had been isolated from the mussel, respectively. The concentrations of free OA, DTX-1 and total OA in which were surpassed the maximum permitted levels in EU. OA and DTX-1 were confirmed to be the main toxins responsible for this DSP outbreak.
authors.affiliation: Wenzhou Center for Disease Control and Prevention, Wenzhou 325001, China. xyzwz123@126.com
authors.forename: Xiuyao
authors.initials: X
authors.lastname: Zhang
authors.forename: Xinxin
authors.initials: X
authors.lastname: Cai
mesh_terms: D000818:Animals; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D005663:Furans / Q000032:analysis / Q000302:isolation & purification; D018942:Macrolides; D049880:Mytilus edulis / Q000737:chemistry*; D009792:Oceans and Seas; D019319:Okadaic Acid / Q000032:analysis / Q000302:isolation & purification / Q000031:analogs & derivatives; D011714:Pyrans / Q000032:analysis / Q000302:isolation & purification; D012758:Shellfish / Q000032:analysis*; D000095702:Polyether Toxins
chemical_list: D005663:Furans; D018942:Macrolides; D019319:Okadaic Acid; D011714:Pyrans; C420916:pectenotoxin-2-seco acid; D000095702:Polyether Toxins; C051904:dinophysistoxin 1