title: Effects of purified glycerol from biodiesel on miRNAs compared to the expression profile of selected mRNAs in Balb/c mice.
abstract: BACKGROUND
  We investigated the effect of corn-derived biodiesel glycerol on microRNAs (miRNAs) and mRNAs, which play a central role in regulating cell survival, apoptosis and carcinogenesis.
  
  
  METHODS
  Inbred Balb/c mice were treated with purified glycerol from biodiesel for 24 hours. After administration, we determined the expressions of miR-21, miR-27a, miR-34a, miR-93, miR-143, miR-146a, miR-148a, miR-155, miR-196a, miR-203, miR-205, miR-221 and nuclear factor kappa-light-chain enhancer of activated B-cells-1 (Nfκb1), mitogen-activated protein kinase-8 (Mapk8) and v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (K-ras) genes in the liver of mice.
  
  
  RESULTS
  We found a parallel altered expression of miRNAs and mRNAs in animals consuming biodiesel glycerol that compared to control mice; these alterations reached significant levels only in few cases.
  
  
  CONCLUSIONS
  Biodiesel glycerol presents no higher risk for carcinogenicity or toxicity.
authors.affiliation: Institute of Public Health, Faculty of Medicine, University of Pécs, Pécs, 12 Szigeti str., 7624 Hungary. eszter.szele@gmail.com
authors.forename: Eszter
authors.initials: E
authors.lastname: Szele
authors.forename: Katalin
authors.initials: K
authors.lastname: Gombos
authors.forename: Krisztina
authors.initials: K
authors.lastname: Juhász
authors.forename: Veronika
authors.initials: V
authors.lastname: Wohler
authors.forename: András
authors.initials: A
authors.lastname: Kovács
authors.forename: István
authors.initials: I
authors.lastname: Ember
mesh_terms: D000818:Animals; D056804:Biofuels; D005260:Female; D020869:Gene Expression Profiling; D005990:Glycerol / Q000302:isolation & purification / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics*; D048055:Mitogen-Activated Protein Kinase 8 / Q000235:genetics; D016328:NF-kappa B / Q000235:genetics; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012737:Sex Factors
chemical_list: D056804:Biofuels; D035683:MicroRNAs; D016328:NF-kappa B; D012333:RNA, Messenger; D048055:Mitogen-Activated Protein Kinase 8; C495971:Hras protein, mouse; D016283:Proto-Oncogene Proteins p21(ras); D005990:Glycerol