title: Expression of small nucleolar RNAs in leukemic cells.
abstract: OBJECTIVE
  Small nucleolar RNAs (snoRNAs) direct sequence-specific modifications to ribosomal RNA. We hypothesized that the expression of snoRNAs may be altered in leukemic cells.
  
  
  METHODS
  The expression of snoRNAs was analyzed in various leukemic cell lines by massive parallel sequencing (SOLiD). Quantitative real-time PCR (RT-qPCR) was used to validate the expression profiles.
  
  
  RESULTS
  Our results show characteristic differences in the expression patterns of snoRNAs between cell lines representing the main subgroups of leukemia, AML, pre-B-ALL and T-ALL, respectively. In RT-qPCR analyses, several snoRNAs were found to be differentially expressed in T-ALL as compared to pre-B-ALL cell lines.
  
  
  CONCLUSIONS
  snoRNAs are differentially expressed in various leukemic cell lines and could, therefore, be potentially useful in the classification of leukemia subgroups.
authors.affiliation: Tampere Center for Child Health Research, University of Tampere School of Medicine and Tampere University Hospital, Biokatu, FIN, Tampere, Finland. kaisa.teittinen@uta.fi
authors.forename: Kaisa J
authors.initials: KJ
authors.lastname: Teittinen
authors.forename: Asta
authors.initials: A
authors.lastname: Laiho
authors.forename: Annemari
authors.initials: A
authors.lastname: Uusimäki
authors.forename: Juha-Pekka
authors.initials: JP
authors.lastname: Pursiheimo
authors.forename: Attila
authors.initials: A
authors.lastname: Gyenesei
authors.forename: Olli
authors.initials: O
authors.lastname: Lohi
mesh_terms: D045744:Cell Line, Tumor; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D018922:HL-60 Cells; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D007938:Leukemia / Q000145:classification / Q000235:genetics / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012334:RNA, Neoplasm / Q000235:genetics*; D020537:RNA, Small Nucleolar / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D012334:RNA, Neoplasm; D020537:RNA, Small Nucleolar