title: Genetic characterisation of a porcine bocavirus detected in domestic pigs in Uganda.
abstract: Porcine bocaviruses (PoBoVs) are small linear ssDNA viruses belonging to the genus bocavirus in the family Parvoviridae. The genome encodes four proteins-the non-structural protein 1 (NS1), the NP1 protein (unknown function) and the two structural proteins VP1 and VP2. In recent years, a number of different highly divergent PoBoV species have been discovered. PoBoVs have been shown to be present in pig populations in Europe, Asia and in the United States of America. In this study, we present the first data of the presence of PoBoV in Africa, specifically in Uganda. A PCR targeting a PoBoV species that have previously been detected in both Sweden and China was used to screen 95 serum samples from domestic pigs in Uganda. Two pigs were found to be positive for this specific PoBoV and the complete coding region was amplified from one of these samples. The amino acid sequence comparison of all these proteins showed a high identity (98-99 %) to the published Chinese sequences (strains: H18 and SX) belonging to the same PoBoV species. The same was true for the Swedish sequences from the same species. To the other PoBoV species the divergence was higher and only a 28-43 % protein sequence identity was seen comparing the different proteins.
authors.affiliation: Section of Virology, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Box 7028, 750 07, Uppsala, Sweden, anne-lie.blomstrom@slu.se.
authors.forename: Anne-Lie
authors.initials: AL
authors.lastname: Blomström
authors.forename: Karl
authors.initials: K
authors.lastname: Ståhl
authors.forename: Ademun Rose
authors.initials: AR
authors.lastname: Okurut
authors.forename: Charles
authors.initials: C
authors.lastname: Masembe
authors.forename: Mikael
authors.initials: M
authors.lastname: Berg
mesh_terms: D000818:Animals; D052660:Bocavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D044967:Serum / Q000821:virology; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D014454:Uganda
chemical_list: D004279:DNA, Viral