title: Frequency and genotype of human papillomavirus among Sudanese patients with head and neck tumours.
abstract: OBJECTIVE
  The aim of this study was to determine the frequency and genotype of human papillomavirus (HPV) infections in head and neck squamous cell carcinomas (HNSCCs) and benign head and neck tumours.
  
  
  METHODS
  A retrospective study was performed on 150 samples of patients diagnosed with HNSCCs and 50 samples obtained from patients diagnosed with benign head and neck tumours. Tumour DNA was amplified using polymerase chain reaction (PCR) with HPV consensus and multiplex primers.
  
  
  RESULTS
  Six of the 150 (4%) HNSCCs were HPV positive. HPV16 was the most prevalent type, with single infections present in 3/6 (50%) cases, whereas HPV18 and HPV33 were detected in 2/6 (33%) and 1/6 (17%), respectively. HPV infections were detected in 3 (50%) cases of oral cavity and 3 (50%) cases of pharynx.
  
  
  CONCLUSIONS
  There was a significant association between HPV infection and HNSCCs (P < 0.05). The present data support the importance of HPV infection in oral and larynx tumours.
authors.affiliation: Department of Pathology, College of Medicine, University of Hail, Hail, Kingdom of Saudi Arabia.
authors.forename: Hussain Gadelkarim
authors.initials: HG
authors.lastname: Ahmed
authors.forename: Saadalnour Abusail
authors.initials: SA
authors.lastname: Mustafa
authors.forename: Faris Margani
authors.initials: FM
authors.lastname: Eltom
authors.forename: Ali Yousef Yahia
authors.initials: AY
authors.lastname: Babiker