title: Replication-coupled passive DNA demethylation for the erasure of genome imprints in mice.
abstract: Genome-wide DNA demethylation, including the erasure of genome imprints, in primordial germ cells (PGCs) is a critical first step to creating a totipotent epigenome in the germ line. We show here that, contrary to the prevailing model emphasizing active DNA demethylation, imprint erasure in mouse PGCs occurs in a manner largely consistent with replication-coupled passive DNA demethylation: PGCs erase imprints during their rapid cycling with little de novo or maintenance DNA methylation potential and no apparent major chromatin alterations. Our findings necessitate the re-evaluation of and provide novel insights into the mechanism of genome-wide DNA demethylation in PGCs.
authors.affiliation: Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.
authors.forename: Saya
authors.initials: S
authors.lastname: Kagiwada
authors.forename: Kazuki
authors.initials: K
authors.lastname: Kurimoto
authors.forename: Takayuki
authors.initials: T
authors.lastname: Hirota
authors.forename: Masashi
authors.initials: M
authors.lastname: Yamaji
authors.forename: Mitinori
authors.initials: M
authors.lastname: Saitou
mesh_terms: D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D022762:CCAAT-Enhancer-Binding Proteins; D002453:Cell Cycle / Q000502:physiology; D049109:Cell Proliferation; D002843:Chromatin / Q000502:physiology; D019175:DNA Methylation / Q000235:genetics / Q000502:physiology*; D017931:DNA Primers / Q000235:genetics; D004261:DNA Replication / Q000502:physiology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D016678:Genome / Q000235:genetics*; D018392:Genomic Imprinting / Q000235:genetics*; D005854:Germ Cells / Q000502:physiology*; D049452:Green Fluorescent Proteins; D007700:Kinetics; D051379:Mice; D046228:Microarray Analysis; D008954:Models, Biological; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D017422:Sequence Analysis, DNA; D044767:Ubiquitin-Protein Ligases
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D002843:Chromatin; D017931:DNA Primers; C485852:Dmap1 protein, mouse; D009687:Nuclear Proteins; D012097:Repressor Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D044767:Ubiquitin-Protein Ligases; C117054:Uhrf1 protein, mouse