title: [Isolation and identification of brine shrimp lethal activities from Derris trifoliata].
abstract: OBJECTIVE
  To study the brine shrimp lethal activities of the roots and stems from Derris trifoliata.
  
  
  METHODS
  The biological activity of the athyl acetate extract was tested by the 2nd-instar of Artemia franciscana by the method of Silica gel column chromatography, Sephadex LH-20 column chromatography, Semipreparative HPLC, and their structures were elucidated by spectral analysis.
  
  
  RESULTS
  Eleven compounds were isolated from the athyl acetate extract, and LD50 s of 12a-hydroxyrotenone, tephrosin, rotenone and deguelin were 0.365, 0.236, 0.060 and 0.734 microg/mL, respectively.
  
  
  CONCLUSIONS
  The results of bioassay show that 12a-hydroxyrotenone, tephrosin,rotenone and deguelin have strong brine shrimp lethal activity.
authors.affiliation: Department of Pharmacy, Medical College, Hunan Normal University, Changsha 410006, China. dangdangli@tom.com
authors.forename: Cheng
authors.initials: C
authors.lastname: Jiang
authors.forename: Ben-qiang
authors.initials: BQ
authors.lastname: Xiong
authors.forename: Xi-min
authors.initials: XM
authors.lastname: Qiu
authors.forename: Sheng-zi
authors.initials: SZ
authors.lastname: Liu
authors.forename: Si
authors.initials: S
authors.lastname: Zhang
authors.forename: Hao
authors.initials: H
authors.lastname: Yin
mesh_terms: D000818:Animals; D001156:Artemia / Q000187:drug effects; D029866:Derris / Q000737:chemistry*; D007306:Insecticides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007814:Larva / Q000187:drug effects; D007928:Lethal Dose 50; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D012402:Rotenone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D007306:Insecticides; D012402:Rotenone; C028404:rotenolone