title: The mRNA level of the galectin-10 of Angiostrongylus cantonensis induced by reactive oxygen stress.
abstract: Galectin plays an important role in host-parasite interactions. In this study, we identified a novel gene encoding galectin-10 (AcGal-10) from the cDNA library of Angiostrongylus cantonensis and characterized its biological role in the parasite. Sequence and phylogeny analysis showed that AcGal-10 is related to other galectin family members with the conserved loci (H(84)-D(86)-R(88)-V(96)-N(98)-W(105)-E(108)-R(110)). The mRNA level of AcGal-10 was expressed in reactive oxygen stress radicals. We have identified two proteins of A. cantonensis galectin-10 gene, one of which was reported (AcGAL10-W) and the others is AcGAL-10-M. In addition, recombinant AcGal-10 (rAcGal-10) was constructed into the pGEX-4T-1 plasmid, purified, and finally confirmed by SDS-PAGE and LC-MS. Hemagglutination assay showed that the minimum concentration of rAcGAL10-W and rAcGAL10-M required for the hemagglutination of BALB/c mice erythrocyte was 25 μg/mL, and the carbohydrate-binding ability showed no difference between rAcGAL10-W and rAcGAL10-M. The mRNA levels of AcGal-10 were indeed expressed higher after stimulation with H(2)O(2) and recombinant A. cantonensis galectin-10. A mutation of AcGal-10 was also found, but there was no significant difference compared with the wild type. Furthermore, we also confirmed that recombinant AcGal-10 plays a role in the activation of the microglia. In conclusion, the report here showed that AcGal-10 may be an important molecule related to infection of A. cantonensis.
authors.affiliation: Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, Guangdong, China.
authors.forename: Ling-Hui
authors.initials: LH
authors.lastname: Liu
authors.forename: Han-Jiang
authors.initials: HJ
authors.lastname: He
authors.forename: Zhi-Yue
authors.initials: ZY
authors.lastname: Lv
authors.forename: Jie
authors.initials: J
authors.lastname: Wei
authors.forename: Xin
authors.initials: X
authors.lastname: Zeng
authors.forename: Jin-Yi
authors.initials: JY
authors.lastname: Liang
authors.forename: Huan-Qin
authors.initials: HQ
authors.lastname: Zheng
authors.forename: Xin-Bing
authors.initials: XB
authors.lastname: Yu
authors.forename: Xi
authors.initials: X
authors.lastname: Sun
authors.forename: Zhong-Dao
authors.initials: ZD
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D017185:Angiostrongylus cantonensis / Q000187:drug effects* / Q000502:physiology*; D000818:Animals; D004912:Erythrocytes / Q000187:drug effects; D037161:Galectins / Q000096:biosynthesis* / Q000235:genetics; D020869:Gene Expression Profiling; D006384:Hemagglutination; D051379:Mice; D008807:Mice, Inbred BALB C; D008958:Models, Molecular; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D010802:Phylogeny; D011485:Protein Binding; D011487:Protein Conformation; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000633:toxicity*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D037161:Galectins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species