title: GTSE1 is a microtubule plus-end tracking protein that regulates EB1-dependent cell migration.
abstract: The regulation of cell migration is a highly complex process that is often compromised when cancer cells become metastatic. The microtubule cytoskeleton is necessary for cell migration, but how microtubules and microtubule-associated proteins regulate multiple pathways promoting cell migration remains unclear. Microtubule plus-end binding proteins (+TIPs) are emerging as important players in many cellular functions, including cell migration. Here we identify a +TIP, GTSE1, that promotes cell migration. GTSE1 accumulates at growing microtubule plus ends through interaction with the EB1+TIP. The EB1-dependent +TIP activity of GTSE1 is required for cell migration, as well as for microtubule-dependent disassembly of focal adhesions. GTSE1 protein levels determine the migratory capacity of both nontransformed and breast cancer cell lines. In breast cancers, increased GTSE1 expression correlates with invasive potential, tumor stage, and time to distant metastasis, suggesting that misregulation of GTSE1 expression could be associated with increased invasive potential.
authors.affiliation: Laboratorio Nazionale The Interuniversity Consortium for Biotechnology, Area Science Park, Trieste, Italy.
authors.forename: Massimilano
authors.initials: M
authors.lastname: Scolz
authors.forename: Per O
authors.initials: PO
authors.lastname: Widlund
authors.forename: Silvano
authors.initials: S
authors.lastname: Piazza
authors.forename: Debora Rosa
authors.initials: DR
authors.lastname: Bublik
authors.forename: Simone
authors.initials: S
authors.lastname: Reber
authors.forename: Leticia Y
authors.initials: LY
authors.lastname: Peche
authors.forename: Yari
authors.initials: Y
authors.lastname: Ciani
authors.forename: Nina
authors.initials: N
authors.lastname: Hubner
authors.forename: Mayumi
authors.initials: M
authors.lastname: Isokane
authors.forename: Martin
authors.initials: M
authors.lastname: Monte
authors.forename: Jan
authors.initials: J
authors.lastname: Ellenberg
authors.forename: Anthony A
authors.initials: AA
authors.lastname: Hyman
authors.forename: Claudio
authors.initials: C
authors.lastname: Schneider
authors.forename: Alexander W
authors.initials: AW
authors.lastname: Bird
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005455:Fluorescent Antibody Technique; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D047468:Immunoprecipitation; D053208:Kaplan-Meier Estimate; D013058:Mass Spectrometry; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism / Q000502:physiology*; D009361:Neoplasm Invasiveness / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; C115080:GTSE1 protein, human; C500985:MAPRE1 protein, human; D008869:Microtubule-Associated Proteins; D034741:RNA, Small Interfering