title: Degradable poly(amidoamine) hydrogels as scaffolds for in vitro culturing of peripheral nervous system cells.
abstract: This paper reports on the synthesis and physico-chemical, mechanical, and biological characterization of two sets of poly(amidoamine) (PAA) hydrogels with potential as scaffolds for in vivo peripheral nerve regeneration. They are obtained by polyaddition of piperazine with N,N'-methylenebis(acrylamide) or 1,4-bis(acryloyl)piperazine with 1,2-diaminoethane as cross-linking agent and exhibit a combination of relevant properties, such as mechanical strength, biocompatibility, biodegradability, ability to induce adhesion and proliferation of Schwann cells (SCs) preserving their viability. Moreover, the most promising hydrogels, that is those deriving from 1,4-bis(acryloyl)piperazine, allow the in vitro growth of the sensitive neurons of the dorsal root ganglia, thus getting around a critical point in the design of conduits for nerve regeneration.
authors.affiliation: Dipartimento di Chimica, Università degli Studi di Milano, via Golgi 19, 20133 Milano, Italy.
authors.forename: Nicolò
authors.initials: N
authors.lastname: Mauro
authors.forename: Amedea
authors.initials: A
authors.lastname: Manfredi
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Ranucci
authors.forename: Patrizia
authors.initials: P
authors.lastname: Procacci
authors.forename: Michele
authors.initials: M
authors.lastname: Laus
authors.forename: Diego
authors.initials: D
authors.lastname: Antonioli
authors.forename: Cristina
authors.initials: C
authors.lastname: Mantovani
authors.forename: Valerio
authors.initials: V
authors.lastname: Magnaghi
authors.forename: Paolo
authors.initials: P
authors.lastname: Ferruti
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005029:Ethylenediamines / Q000737:chemistry; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects*; D020100:Hydrogels / Q000737:chemistry*; D009416:Nerve Regeneration; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D010879:Piperazines / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects*; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D000178:Acrylamides; D001672:Biocompatible Materials; D005029:Ethylenediamines; D020100:Hydrogels; D010879:Piperazines; C531249:Poly(amidoamine); D011073:Polyamines; C031234:ethylenediamine