title: The radical SAM enzyme spore photoproduct lyase employs a tyrosyl radical for DNA repair.
abstract: The spore photoproduct lyase is a radical SAM enzyme, which repairs 5-(α-thyminyl)-5,6-dihydrothymidine. Here we show that the enzyme establishes a complex radical transfer cascade and creates a cysteine and a tyrosyl radical dyade to establish repair. This allows the enzyme to solve topological and energetic problems associated with the radical based repair reaction.
authors.affiliation: Center for Integrative Protein Science at the Department for Chemistry, Ludwig-Maximilians-Universität, Butenandtstr. 5-13, 81377 Munich, Germany.
authors.forename: Andrea Christa
authors.initials: AC
authors.lastname: Kneuttinger
authors.forename: Korbinian
authors.initials: K
authors.lastname: Heil
authors.forename: Gengo
authors.initials: G
authors.lastname: Kashiwazaki
authors.forename: Thomas
authors.initials: T
authors.lastname: Carell
mesh_terms: D003545:Cysteine / Q000737:chemistry; D004260:DNA Repair; D056506:Geobacillus / Q000201:enzymology; D008956:Models, Chemical; D011506:Proteins / Q000378:metabolism*; D013171:Spores, Bacterial / Q000528:radiation effects; D013936:Thymidine / Q000031:analogs & derivatives* / Q000737:chemistry; D014443:Tyrosine / Q000737:chemistry
chemical_list: C581343:5-(alpha-thyminyl)-5,6-dihydrothymidine; D011506:Proteins; D014443:Tyrosine; C088014:spore photoproduct lyase; D003545:Cysteine; D013936:Thymidine