title: Molecular characterization, tissue distribution and kinetic analysis of carnitine palmitoyltransferase I in juvenile yellow catfish Pelteobagrus fulvidraco.
abstract: Up to date, only limited information is available on genetically and functionally different isoforms of CPT I enzyme in fish. In the study, molecular characterization and their tissue expression profile of three CPT Iα isoforms (CPT Iα1a, CPT Iα1b and CPT Iα2a) and a CPT Iβ isoform from yellow catfish Pelteobagrus fulvidraco is determined. The activities and kinetic features of CPT I from several tissues have also been analyzed. The four CPT I isoforms in yellow catfish present distinct differences in amino acid sequences and structure. They are widely expressed in liver, heart, white muscle, spleen, intestine and mesenteric adipose tissue of yellow catfish at the mRNA level, but with the varying levels. CPT I activity and kinetics show tissue-specific differences stemming from co-expression of different isoforms, indicating more complex pathways of lipid utilization in fish than in mammals, allowing for precise control of lipid oxidation in individual tissue.
authors.affiliation: Fishery College, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Jia-Lang
authors.initials: JL
authors.lastname: Zheng
authors.forename: Zhi
authors.initials: Z
authors.lastname: Luo
authors.forename: Qing-Ling
authors.initials: QL
authors.lastname: Zhu
authors.forename: Qi-Liang
authors.initials: QL
authors.lastname: Chen
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gong
mesh_terms: D000595:Amino Acid Sequence; D002334:Carnitine O-Palmitoyltransferase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D015227:Lipid Peroxidation / Q000235:genetics; D008099:Liver / Q000378:metabolism; D008929:Mitochondria, Heart / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D020033:Protein Isoforms / Q000235:genetics* / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D020033:Protein Isoforms; D002334:Carnitine O-Palmitoyltransferase