title: An Arabidopsis cell growth defect factor-related protein, CRS, promotes plant senescence by increasing the production of hydrogen peroxide.
abstract: Arabidopsis thaliana Cell Growth Defect factor 1 (Cdf1) has been implicated in promotion of proapoptotic Bax-like cell death via the induction of reactive oxygen species (ROS). Here we report a conserved function of a chloroplast-targeting Cdf-related gene Responsive to Senescence (CRS) using CRS overexpression and loss of function in plants as well as CRS heterologous expression in yeast. CRS expression was strongly induced in senescent leaves, suggesting its main functions during plant senescence. CRS expression in yeast mitochondria increased the ROS level and led to cell death in a manner similar to Cdf1. In whole plants, overexpression of CRS caused the loss of chlorophylls (Chls) and the rapid onset of leaf senescence, while the lack of CRS led to the delay of leaf senescence in a loss-of-function mutant, crs. The higher and lower accumulation of H(2)O(2) was correlated with early and late senescence in CRS-overexpressing and crs mutant plants, respectively. Furthermore, expression of senescence-related marker genes and metacaspase genes was induced in CRS-overexpressing plants in response to dark. Our findings suggest that CRS plays a key role in the leaf senescence process that accompanies H(2)O(2) accumulation resulting in cell death promotion.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
authors.forename: Mei Hua
authors.initials: MH
authors.lastname: Cui
authors.forename: Sung Han
authors.initials: SH
authors.lastname: Ok
authors.forename: Kyoung Shin
authors.initials: KS
authors.lastname: Yoo
authors.forename: Kwang Wook
authors.initials: KW
authors.lastname: Jung
authors.forename: Sang Dong
authors.initials: SD
authors.lastname: Yoo
authors.forename: Jeong Sheop
authors.initials: JS
authors.lastname: Shin
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D016923:Cell Death / Q000235:genetics; D002736:Chloroplasts / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D003624:Darkness; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000166:cytology / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D015003:Yeasts / Q000166:cytology / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; C582238:CRS protein, Arabidopsis; D004268:DNA-Binding Proteins; C098796:Sen1 protein, Arabidopsis; C496136:WRKY53 protein, Arabidopsis; D006861:Hydrogen Peroxide