title: An enhanced sensing platform for ultrasensitive impedimetric detection of target genes based on ordered FePt nanoparticles decorated carbon nanotubes.
abstract: We demonstrate a novel high-performance DNA hybridization biosensor with a carbon nanotubes (CNTs)-based nanocomposite membrane as the enhanced sensing platform. The platform was constructed by homogenously distributing ordered FePt nanoparticles (NPs) onto the CNTs matrix. The surface structure and electrochemical performance of the FePt/CNTs nanocomposite membrane were systematically investigated. Such a nanostructured composite membrane platform could combine with the advantages of FePt NPs and CNTs, greatly facilitate the electron-transfer process and the sensing behavior for DNA detection, leading to excellent sensitivity and selectivity. The complementary target genes from acute promyelocytic leukemia could be quantified in a wide range of 1.0×10⁻¹² mol/L to 1.0×10⁻⁶ mol/L using electrochemical impedance spectroscopy, and the detection limit was 2.1×10⁻¹³ mol/L under the optimal conditions. In addition, the DNA electrochemical biosensor was highly selective to discriminate single-base or double-base mismatched sequences.
authors.affiliation: Key Laboratory of Resources and Enviromental Analytical Chemistry in Universities of Shandong, School of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China. zhangweiqust@126.com
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Peisong
authors.initials: P
authors.lastname: Zong
authors.forename: Xiuwen
authors.initials: X
authors.lastname: Zheng
authors.forename: Libin
authors.initials: L
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry; D017097:Electric Impedance; D006046:Gold / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D015514:Oncogene Proteins, Fusion / Q000737:chemistry / Q000302:isolation & purification; D010984:Platinum / Q000737:chemistry
chemical_list: D037742:Nanotubes, Carbon; D015514:Oncogene Proteins, Fusion; C093476:promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein; D010984:Platinum; D006046:Gold; D004247:DNA; D007501:Iron