title: Molecular cloning and functional characterization  of a mouse ccl6 analog gene in the rat.
abstract: Suppression subtractive hybridization was  used to analyze differential expression of genes in rat peritoneal macrophages  after granulocyte macrophage colony-stimulating factor treatment. We identified  and cloned the mouse C10 analog gene in the rat, and named it as ccl6.  The full-length cDNA of rat ccl6 was 467 bp, which contains a  single-open reading frame and encodes 116 amino acid residues. Compared with  other C-C chemokines, the rat ccl6 gene had an unusual four-exon genome  structure instead of the typical three exons, it had the highest homology with  murine ccl6. The rat ccl6 gene was localized on chromosome 10,  where most of the C-C chemokine superfamily members are located. The  recombinant rat C-C chemokine ligand 6 (CCL6) protein was expressed by the  pGEX4T-1 plasmid in Escherichia coli BL21. The purified recombinant  protein had bioactivity similar to that of mouse CCL6, which is a  chemoattractant for macrophages and lymphocytes, but not for neutrophils.
authors.affiliation: Department of Pharmacy, Beijing Military Region, General Hospital, Beijing, China. jiangnan7757@sohu.com
authors.forename: N
authors.initials: N
authors.lastname: Jiang
authors.forename: Y H
authors.initials: YH
authors.lastname: Zheng
authors.forename: X J
authors.initials: XJ
authors.lastname: Chen
authors.forename: C
authors.initials: C
authors.lastname: Qiu
authors.forename: X F
authors.initials: XF
authors.lastname: Zhang
authors.forename: S H
authors.initials: SH
authors.lastname: Wen
authors.forename: G X
authors.initials: GX
authors.lastname: Bian
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019742:Chemokines, CC / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D002633:Chemotaxis / Q000187:drug effects; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons / Q000235:genetics; D006824:Hybridization, Genetic / Q000187:drug effects; D007438:Introns / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects; D010802:Phylogeny; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: C579176:Ccl6 protein, rat; D019742:Chemokines, CC; D011994:Recombinant Proteins; C070959:Ccl6 protein, mouse