title: Proteomic approaches identify members of cofilin pathway involved in oral tumorigenesis.
abstract: The prediction of tumor behavior for patients with oral carcinomas remains a challenge for clinicians. The presence of lymph node metastasis is the most important prognostic factor but it is limited in predicting local relapse or survival. This highlights the need for identifying biomarkers that may effectively contribute to prediction of recurrence and tumor spread. In this study, we used one- and two-dimensional gel electrophoresis, mass spectrometry and immunodetection methods to analyze protein expression in oral squamous cell carcinomas. Using a refinement for classifying oral carcinomas in regard to prognosis, we analyzed small but lymph node metastasis-positive versus large, lymph node metastasis-negative tumors in order to contribute to the molecular characterization of subgroups with risk of dissemination. Specific protein patterns favoring metastasis were observed in the "more-aggressive" group defined by the present study. This group displayed upregulation of proteins involved in migration, adhesion, angiogenesis, cell cycle regulation, anti-apoptosis and epithelial to mesenchymal transition, whereas the "less-aggressive" group was engaged in keratinocyte differentiation, epidermis development, inflammation and immune response. Besides the identification of several proteins not yet described as deregulated in oral carcinomas, the present study demonstrated for the first time the role of cofilin-1 in modulating cell invasion in oral carcinomas.
authors.affiliation: Departamento de Biologia Molecular, Faculdade de Medicina, FAMERP, São José do Rio Preto, SP, Brazil.
authors.forename: Giovana M
authors.initials: GM
authors.lastname: Polachini
authors.forename: Lays M
authors.initials: LM
authors.lastname: Sobral
authors.forename: Ana M C
authors.initials: AM
authors.lastname: Mercante
authors.forename: Adriana F
authors.initials: AF
authors.lastname: Paes-Leme
authors.forename: Flávia C A
authors.initials: FC
authors.lastname: Xavier
authors.forename: Tiago
authors.initials: T
authors.lastname: Henrique
authors.forename: Douglas M
authors.initials: DM
authors.lastname: Guimarães
authors.forename: Alessandra
authors.initials: A
authors.lastname: Vidotto
authors.forename: Erica E
authors.initials: EE
authors.lastname: Fukuyama
authors.forename: José F
authors.initials: JF
authors.lastname: Góis-Filho
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Cury
authors.forename: Otávio A
authors.initials: OA
authors.lastname: Curioni
authors.forename: Pedro
authors.initials: P
authors.lastname: Michaluart
authors.forename: Adriana M A
authors.initials: AM
authors.lastname: Silva
authors.forename: Victor
authors.initials: V
authors.lastname: Wünsch-Filho
authors.forename: Fabio D
authors.initials: FD
authors.lastname: Nunes
authors.forename: Andréia M
authors.initials: AM
authors.lastname: Leopoldino
authors.forename: Eloiza H
authors.initials: EH
authors.lastname: Tajara
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D051338:Cofilin 1 / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology; D009361:Neoplasm Invasiveness; D040901:Proteomics
chemical_list: D051338:Cofilin 1