title: Molecular taxonomy of Anopheles (Nyssorhynchus) benarrochi (Diptera: Culicidae) and malaria epidemiology in southern Amazonian Peru.
abstract: Anopheline specimens were collected in 2011 by human landing catch, Shannon and CDC traps from the malaria endemic localities of Santa Rosa and San Pedro in Madre de Dios Department, Peru. Most specimens were either Anopheles (Nyssorhynchus) benarrochi B or An. (Nys.) rangeli, confirmed by polymerase chain reaction-restriction fragment length polymorphism-internal transcribed spacer 2 (PCR-RFLP-ITS2) and, for selected individuals, ITS2 sequences. A few specimens from Lupuna, Loreto Department, northern Amazonian Peru, were also identified as An. benarrochi B. A statistical parsimony network using ITS2 sequences confirmed that all Peruvian An. benarrochi B analyzed were identical to those in GenBank from Putumayo, southern Colombia. Sequences of the mtDNA COI BOLD region of specimens from all three Peruvian localities were connected using a statistical parsimony network, although there were multiple mutation steps between northern and southern Peruvian sequences. A Bayesian inference of concatenated Peruvian sequences of ITS2 + COI detected a single clade with very high support for all An. benarrochi B except one individual from Lupuna that was excluded. No samples were positive for Plasmodium by CytB-PCR.
authors.affiliation: Griffin Laboratory, The Wadsworth Center, New York State Department of Health, Slingerlands, NY 12159, USA. jconn@wadsworth.org
authors.forename: Jan E
authors.initials: JE
authors.lastname: Conn
authors.forename: Marta
authors.initials: M
authors.lastname: Moreno
authors.forename: Marlon
authors.initials: M
authors.lastname: Saavedra
authors.forename: Sara A
authors.initials: SA
authors.lastname: Bickersmith
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Knoll
authors.forename: Roberto
authors.initials: R
authors.lastname: Fernandez
authors.forename: Hubert
authors.initials: H
authors.lastname: Vera
authors.forename: Roxanne G
authors.initials: RG
authors.lastname: Burrus
authors.forename: Andres G
authors.initials: AG
authors.lastname: Lescano
authors.forename: Juan Francisco
authors.initials: JF
authors.lastname: Sanchez
authors.forename: Esteban
authors.initials: E
authors.lastname: Rivera
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Vinetz
mesh_terms: D000818:Animals; D000852:Anopheles / Q000145:classification* / Q000235:genetics*; D001499:Bayes Theorem; D004272:DNA, Mitochondrial / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D006801:Humans; D007303:Insect Vectors / Q000145:classification* / Q000235:genetics*; D008288:Malaria / Q000453:epidemiology*; D010568:Peru / Q000453:epidemiology; D010961:Plasmodium / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D021903:DNA, Ribosomal Spacer