title: ZrO₂ surface chemically coated with hyaluronic acid hydrogel loading GDF-5 for osteogenesis in dentistry.
abstract: The objective of this study was to modify zirconium dioxide (ZrO(2)) with photo-cured hyaluronic acid hydrogel (pcHAgel), and to subsequently evaluate the bone regeneration potential of the modified ZrO(2). In the present study, HA grafted onto a ZrO(2) substrate was investigated for its biocompatibility and other properties. We describe the positive influences of ZrO(2) surface-modified with pcHAgel (Zr-3) containing two different loads of growth and differentiation factor-5 (GDF-5) to aid new bone formation as compared to the same amount of BMP-2 (Zr-4-7). We characterized the Zr-3 for their surface morphology and chemical properties. Atomic force microscopy (AFM), scanning electron microscope (SEM), and X-ray photoelectron spectroscopy (XPS) showed that the pcHAgel was successfully grafted onto the ZrO(2) surface. The sustained release of GDF-5 and BMP-2 were observed to occur in the Zr-4-7. In vitro cell tests showed a higher level of MG63 cell proliferation and differentiation on Zr-4-7 than on Zr-3. The Zr-3 is a good biomaterial to deliver osteogenic differentiation factors such as BMP-2 and GDF-5, and GDF-5 can be useful as an effective alternative to aid new bone formation as compared to BMP-2.
authors.affiliation: Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
authors.forename: Min Soo
authors.initials: MS
authors.lastname: Bae
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Kim
authors.forename: Jung Bok
authors.initials: JB
authors.lastname: Lee
authors.forename: Dong Nyoung
authors.initials: DN
authors.lastname: Heo
authors.forename: Dae Hyeok
authors.initials: DH
authors.lastname: Yang
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Kung-Rock
authors.initials: KR
authors.lastname: Kwon
authors.forename: Jae Beum
authors.initials: JB
authors.lastname: Bang
authors.forename: Hojae
authors.initials: H
authors.lastname: Bae
authors.forename: Il Keun
authors.initials: IK
authors.lastname: Kwon
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D001861:Bone Regeneration / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003813:Dentistry; D055428:Growth Differentiation Factor 5 / Q000378:metabolism; D006820:Hyaluronic Acid / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000138:chemical synthesis / Q000737:chemistry; D010012:Osteogenesis; D056951:Photoelectron Spectroscopy; D013499:Surface Properties; D015040:Zirconium / Q000737:chemistry / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D055428:Growth Differentiation Factor 5; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D006820:Hyaluronic Acid; D015040:Zirconium; C028541:zirconium oxide