title: Gastrointestinal absorption and metabolism of apple polyphenols ex vivo by the pig intestinal mucosa in the Ussing chamber.
abstract: Polyphenols contained in food have various positive effects on human health. The absorption and metabolism of polyphenols in the intestinal tract needs to be studied to estimate these effects. The Ussing chamber technique was used to investigate the transport behavior of apple polyphenols through pig small intestinal mucosa, which served as a model for human gastrointestinal mucosa. The identities and concentrations of polyphenols and their metabolites in the half-chambers (luminal and basolateral) within an incubation period of 4 h were determined by HPLC-MS/MS and HPLC-DAD (DAD = diode-array detection). Flux values were also measured. It was found that 5-caffeoylquinic acid and caffeic acid were absorbed and translocated to the basolateral side (1.9 and 3.7%, respectively), but other compounds, including glycosides of phloretin and quercetin, were observed without translocation. A Ussing chamber utilizing pig small intestinal mucosa is a suitable model for assessing the effect of apple polyphenols on mucosal integrity and nutrition absorption across porcine mucosa.
authors.affiliation: Department of Chemistry, Division of Food Chemistry and Toxicology, Molecular Nutrition, University of Kaiserslautern, Kaiserslautern, Germany.
authors.forename: Hannah
authors.initials: H
authors.lastname: Deusser
authors.forename: Dorothee
authors.initials: D
authors.lastname: Rogoll
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Scheppach
authors.forename: Antje
authors.initials: A
authors.lastname: Volk
authors.forename: Ralph
authors.initials: R
authors.lastname: Melcher
authors.forename: Elke
authors.initials: E
authors.lastname: Richling
mesh_terms: D000818:Animals; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism*; D027845:Malus / Q000737:chemistry*; D059808:Polyphenols / Q000378:metabolism*; D013552:Swine
chemical_list: D059808:Polyphenols