title: Evaluation of the effect of hydroxypropyl-β-cyclodextrin on topical administration of milk thistle extract.
abstract: Two water in oil emulsions composed by eudermic ingredients as glycerin, cocoa butter, almond oil and a variety of lipids, were enriched respectively with milk thistle dry extract (MT) or with a binary complex composed by MT and hydroxypropyl-β-cyclodextrin (HP) (1:4 w/w) correspondent to 1% (w/w) in sylimarine in order to obtain two different emulsions designed for the skin delivery and determine influence of hydroxypropyl-β-cyclodextrin on the extract delivery and permeation. Uv-vis spectrophotometric analyses demonstrated that phytocomplex formation influences the finding of MT after the complexation process and the in vitro antioxidant activity. Further in vitro and ex vivo experiments demonstrated that the penetration capability of MT from formulations is strictly influenced by the phytocomplex able to control MT permeation; moreover phytocomplex increases flavonoids stability during the in vitro tests. Additionally, in vivo studies showed that the penetration into the stratum corneum of the active ingredients is effectively achieved by the phytocomplex formation, in fact about 80% of MT is absorbed by the skin along 1h despite the 30% of MT not complexed absorbed during the same period.
authors.affiliation: Department of Chemistry and Pharmacy, University of Sassari, via Muroni 23/a, 07100 Sassari, Italy.
authors.forename: Gianpiera
authors.initials: G
authors.lastname: Spada
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Gavini
authors.forename: Massimo
authors.initials: M
authors.lastname: Cossu
authors.forename: Giovanna
authors.initials: G
authors.lastname: Rassu
authors.forename: Antonio
authors.initials: A
authors.lastname: Carta
authors.forename: Paolo
authors.initials: P
authors.lastname: Giunchedi
mesh_terms: D000073738:2-Hydroxypropyl-beta-cyclodextrin; D000287:Administration, Topical; D000328:Adult; D000818:Animals; D004041:Dietary Fats / Q000494:pharmacology; D016503:Drug Delivery Systems; D004655:Emulsions / Q000737:chemistry; D005260:Female; D005990:Glycerol / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008875:Middle Aged; D020944:Silybum marianum / Q000737:chemistry*; D010539:Permeability / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology; D012867:Skin / Q000187:drug effects; D012869:Skin Absorption / Q000187:drug effects*; D013552:Swine; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D004041:Dietary Fats; D004655:Emulsions; D010936:Plant Extracts; D010938:Plant Oils; D047392:beta-Cyclodextrins; D000073738:2-Hydroxypropyl-beta-cyclodextrin; C052387:cocoa butter; C068582:almond oil; D005990:Glycerol