title: MyD88 and TLR9 are required for early control of Brucella ovis infection in mice.
abstract: Brucella ovis is an important cause of epididymitis in rams, which results in impaired fertility and economic losses. This study demonstrated the role of TLR during the acute phase of infection in the mouse model. C57BL/6 wild type and TLR2(-/-), TLR4(-/-), TLR9(-/-), and MyD88(-/-) mice were infected with B. ovis and bacteriology, histopathology, and pro-inflammatory gene expression were evaluated at 7days post-infection. MyD88(-/-) mice had higher bacterial loads in the spleen when compared to wild type mice. This enhanced susceptibility was associated with decreased inflammatory response in the liver. TLR9(-/-) mice also had higher bacterial loads when compared to wild type mice, but, surprisingly, they developed stronger inflammatory response. TLR2(-/-) and TLR4(-/-) mice were as susceptible as wild type mice to B. ovis infection. Therefore, MyD88 and TLR9 are required for controlling B. ovis multiplication during the early stages of infection.
authors.affiliation: Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, MG, Brazil.
authors.forename: Ana Luiza S
authors.initials: AL
authors.lastname: Vieira
authors.forename: Teane M A
authors.initials: TM
authors.lastname: Silva
authors.forename: Juliana P S
authors.initials: JP
authors.lastname: Mol
authors.forename: Sergio C
authors.initials: SC
authors.lastname: Oliveira
authors.forename: Renato L
authors.initials: RL
authors.lastname: Santos
authors.forename: Tatiane A
authors.initials: TA
authors.lastname: Paixão
mesh_terms: D000818:Animals; D058491:Bacterial Load / Q000662:veterinary; D042061:Brucella ovis; D002006:Brucellosis / Q000235:genetics* / Q000276:immunology; D004198:Disease Susceptibility / Q000662:veterinary; D005260:Female; D007113:Immunity, Innate / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout / Q000235:genetics; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics / Q000502:physiology*; D013154:Spleen / Q000382:microbiology; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000502:physiology; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000502:physiology; D051217:Toll-Like Receptor 9 / Q000235:genetics / Q000502:physiology*
chemical_list: D053594:Myeloid Differentiation Factor 88; C493486:Tlr2 protein, mouse; C493487:Tlr4 protein, mouse; C495370:Tlr9 protein, mouse; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051217:Toll-Like Receptor 9