title: Quantification of the effects of antibodies on the extra- and intracellular dynamics of Salmonella enterica.
abstract: Antibodies are known to be essential in controlling Salmonella infection, but their exact role remains elusive. We recently developed an in vitro model to investigate the relative efficiency of four different human immunoglobulin G (IgG) subclasses in modulating the interaction of the bacteria with human phagocytes. Our results indicated that different IgG subclasses affect the efficacy of Salmonella uptake by human phagocytes. In this study, we aim to quantify the effects of IgG on intracellular dynamics of infection by combining distributions of bacterial numbers per phagocyte observed by fluorescence microscopy with a mathematical model that simulates the in vitro dynamics. We then use maximum likelihood to estimate the model parameters and compare them across IgG subclasses. The analysis reveals heterogeneity in the division rates of the bacteria, strongly suggesting that a subpopulation of intracellular Salmonella, while visible under the microscope, is not dividing. Clear differences in the observed distributions among the four IgG subclasses are best explained by variations in phagocytosis and intracellular dynamics. We propose and compare potential factors affecting the replication and death of bacteria within phagocytes, and we discuss these results in the light of recent findings on dormancy of Salmonella.
authors.affiliation: Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, UK. or226@cam.ac.uk
authors.forename: Olivier
authors.initials: O
authors.lastname: Restif
authors.forename: Yun S
authors.initials: YS
authors.lastname: Goh
authors.forename: Matthieu
authors.initials: M
authors.lastname: Palayret
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Grant
authors.forename: Trevelyan J
authors.initials: TJ
authors.lastname: McKinley
authors.forename: Michael R
authors.initials: MR
authors.lastname: Clark
authors.forename: Pietro
authors.initials: P
authors.lastname: Mastroeni
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D066298:In Vitro Techniques; D016013:Likelihood Functions; D008856:Microscopy, Fluorescence; D018448:Models, Immunological; D010586:Phagocytes / Q000276:immunology*; D012486:Salmonella typhimurium / Q000254:growth & development / Q000276:immunology* / Q000378:metabolism
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D049452:Green Fluorescent Proteins