title: Promotion of skeletal muscle repair in a rat skeletal muscle injury model by local injection of human adipose tissue-derived regenerative cells.
abstract: Human adipose tissue-derived regenerative cells (ADRCs) can be isolated easily and aseptically from unwanted subcutaneous fat without culturing. ADRCs have been used in clinical cosmetic therapy. In addition, they are expected to be an attractive and feasible source of cell-based therapies in regenerative medicine. Therefore, this paper investigates whether transplantation of human adult ADRCs into skeletal muscle injury models promotes the repair of muscle tissues. This was done by locally injecting human ADRCs into an injured site after laceration of the nude-rat tibialis anterior muscle. Phosphate-buffered saline (PBS) and bone marrow mononuclear cells (MNCs) were injected as negative and positive controls, respectively. After injury, recovery of muscle strength was accelerated by transplantation of ADRCs compared to administration of PBS and MNCs. Moreover, transplantation of ADRCs also enhanced angiogenesis and myogenesis, but the number of vascular and muscular cells labeled with the human cell-specific maker was limited at the injury site. Results showed that transplantation of ADRCs into a skeletal muscle injury model promoted repair of muscle tissues in a paracrine manner rather than differentiation of itself into blood vessels and myofibres. Thus, it is believed that ADRCs are a useful and feasible cell source not only for cosmetic therapy but also for regenerative therapy.
authors.affiliation: Department of Orthopedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Mori
authors.affiliation: Department of Orthopedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Japan.|Division of Regeneration and Medicine, Hiroshima University Hospital, Japan.
authors.forename: Naosuke
authors.initials: N
authors.lastname: Kamei
authors.affiliation: Department of Orthopedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Okawa
authors.affiliation: Department of Orthopedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Nakabayashi
authors.affiliation: Department of Plastic and Reconstructive Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Yokota
authors.affiliation: Division of Regeneration and Medicine, Hiroshima University Hospital, Japan.
authors.forename: Yukihito
authors.initials: Y
authors.lastname: Higashi
authors.affiliation: Department of Orthopedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Japan.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Ochi
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D000818:Animals; D002454:Cell Differentiation; D064987:Cell- and Tissue-Based Therapy; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008954:Models, Biological; D018482:Muscle, Skeletal / Q000293:injuries / Q000503:physiopathology*; D051381:Rats; D011916:Rats, Inbred F344; D012038:Regeneration; D055815:Young Adult