title: Highly permissive subclone of the porcine kidney cell line for porcine circovirus type 2 production.
abstract: This study established a highly permissive and decontaminated cell line for growing porcine circovirus type 2 (PCV2). A porcine kidney-15 cell line (PK-15) contaminated with porcine circovirus type 1 (PCV1) was decontaminated by neutralizing with rabbit anti-PCV1 hyperimmune serum. Subsequently, by limiting dilution and cell subcloning, four PCV1-free monoclonal cells were grown to monolayers. Each cell clone and PK-15 cell were infected with PCV2. The PKKC cell clone yielded up to 10(6.8)TCID(50)/ml at 6 days post-infection. In addition, PKKC was free of extraneous viral contamination and exhibited a cytopathic effect (CPE) to PCV2 at 6 days post-infection. The advantages of the PKKC cell are that it can grow a high PCV2 titer and exhibit CPE; therefore, it can be used for PCV2 cultivation, vaccine production, and diagnostic purposes.
authors.affiliation: School of Veterinary Medicine, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: Hsu-Chung Gabriel
authors.initials: HC
authors.lastname: Chen
authors.forename: Tsun-Yung
authors.initials: TY
authors.lastname: Kuo
authors.forename: Ying-Chen
authors.initials: YC
authors.lastname: Yang
authors.forename: Chung-Chin
authors.initials: CC
authors.lastname: Wu
authors.forename: Shiow-Suey
authors.initials: SS
authors.lastname: Lai
mesh_terms: D000818:Animals; D002460:Cell Line; D017925:Circovirus / Q000254:growth & development*; D002999:Clone Cells / Q000821:virology; D007668:Kidney / Q000166:cytology; D013552:Swine; D013997:Time Factors; D019562:Viral Load; D014776:Virus Cultivation / Q000379:methods