title: Colocalization of quantum dots by reactive molecules carried by motor proteins on polarized microtubule arrays.
abstract: The field of microfluidics has drastically contributed to downscale the size of benchtop experiments to the dimensions of a chip without compromising results. However, further miniaturization and the ability to directly manipulate individual molecules require a platform that permits organized molecular transport. The motor proteins and microtubules that carry out orderly intracellular transport are ideal for driving in vitro nanotransport. Here, we demonstrate that a reconstruction of the cellular kinesin/dynein-microtubule system in nanotracks provides a molecular total analysis system (MTAS) to control massively parallel chemical reactions. The mobility of kinesin and a microtubule dissociation method enable orientation of a microtubule in an array for directed transport of reactive molecules carried by kinesin or dynein. The binding of glutathione S-transferase (GST) to glutathione (GSH) and the binding of streptavidin to biotin are visualized as colocalizations of quantum dots (Q-dots) when motor motilities bring them into contact. The organized nanotransport demonstrated here suggests the feasibility of using our platform to perform parallel biochemical reactions focused at the molecular level.
authors.affiliation: Department of Micro Engineering, Kyoto University, Yoshida-Honmachi, Sakyo, Kyoto 606-8501, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Fujimoto
authors.forename: Masuto
authors.initials: M
authors.lastname: Kitamura
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Yokokawa
authors.forename: Isaku
authors.initials: I
authors.lastname: Kanno
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Kotera
authors.forename: Ryuji
authors.initials: R
authors.lastname: Yokokawa
mesh_terms: D008422:Materials Testing; D008870:Microtubules / Q000737:chemistry* / Q000648:ultrastructure*; D020409:Molecular Motor Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D020409:Molecular Motor Proteins