title: Analysis of chemokine receptor CCR7 expression on porcine blood T lymphocytes using a CCL19-Fc fusion protein.
abstract: The chemokine receptor CCR7 has been a useful marker for the characterization of human and mouse T cell subsets. We have produced the porcine CCR7 ligand CCL19 fused to the human IgG1 Fc fragment, and used it to analyse CCR7 expression in swine. CCL19-Fc bound to and induced the migration of cells expressing porcine CCR7 but not of untransfected cells, corroborating its specificity. On blood lymphocytes, CCL19-Fc labelled the majority of CD4(+) T cells expressing the 2E3 marker, associated with a naïve phenotype, whereas the 2E3(-) cells were mostly negative. Among CD8(+) T cells CCL19-Fc labelled two subsets: one, CD8β(hi) CD11a(lo) CD45RA(+), perforin(-/lo) , which produced low amounts of IFN-γ after stimulation, which might correspond to naïve cells; and a second small population of CD8β(lo) cells which expressed high levels of CD11a, and were mostly CD45RA(-), a phenotype which resembles that of human central memory T cells.
authors.affiliation: Dpto. de Biotecnología, INIA, Ctra de la Coruña Km 7.5, 28040 Madrid, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Moreno
authors.forename: B
authors.initials: B
authors.lastname: Alvarez
authors.forename: P
authors.initials: P
authors.lastname: Martínez
authors.forename: H
authors.initials: H
authors.lastname: Uenishi
authors.forename: C
authors.initials: C
authors.lastname: Revilla
authors.forename: A
authors.initials: A
authors.lastname: Ezquerra
authors.forename: F
authors.initials: F
authors.lastname: Alonso
authors.forename: J
authors.initials: J
authors.lastname: Domínguez
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001773:Blood Cells / Q000276:immunology*; D002478:Cells, Cultured; D054415:Chemokine CCL19 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000235:genetics / Q000378:metabolism*; D007156:Immunologic Memory; D016130:Immunophenotyping; D015202:Protein Engineering; D054400:Receptors, CCR7 / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D013552:Swine / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D054415:Chemokine CCL19; D007141:Immunoglobulin Fc Fragments; D054400:Receptors, CCR7; D011993:Recombinant Fusion Proteins