title: Metabolic response to Klebsiella pneumoniae infection in an experimental rat model.
abstract: Bacteremia, the presence of viable bacteria in the blood stream, is often associated with several clinical conditions. Bacteremia can lead to multiple organ failure if managed incorrectly, which makes providing suitable nutritional support vital for reducing bacteremia-associated mortality. In order to provide such information, we investigated the metabolic consequences of a Klebsiella pneumoniae (K. pneumoniae) infection in vivo by employing a combination of (1)H nuclear magnetic resonance spectroscopy and multivariate data analysis. K. pneumoniae was intravenously infused in rats; urine and plasma samples were collected at different time intervals. We found that K. pneumoniae-induced bacteremia stimulated glycolysis and the tricarboxylic acid cycle and also promoted oxidation of fatty acids and creatine phosphate to facilitate the energy-demanding host response. In addition, K. pneumoniae bacteremia also induced anti-endotoxin, anti-inflammatory and anti-oxidization responses in the host. Furthermore, bacteremia could cause a disturbance in the gut microbiotal functions as suggested by alterations in a range of amines and bacteria-host co-metabolites. Our results suggest that supplementation with glucose and a high-fat and choline-rich diet could ameliorate the burdens associated with bacteremia. Our research provides underlying pathological processes of bacteremia and a better understanding of the clinical and biochemical manifestations of bacteremia.
authors.affiliation: Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Centre for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, PR China.
authors.forename: Fangcong
authors.initials: F
authors.lastname: Dong
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Lulu
authors.initials: L
authors.lastname: Zhang
authors.forename: Huiru
authors.initials: H
authors.lastname: Tang
authors.forename: Jieshou
authors.initials: J
authors.lastname: Li
authors.forename: Yulan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D016470:Bacteremia / Q000097:blood / Q000378:metabolism / Q000382:microbiology / Q000652:urine; D002097:C-Reactive Protein / Q000378:metabolism; D002116:Calcitonin / Q000378:metabolism; D015169:Colony Count, Microbial; D016002:Discriminant Analysis; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D007710:Klebsiella Infections / Q000097:blood / Q000378:metabolism* / Q000382:microbiology* / Q000652:urine; D007711:Klebsiella pneumoniae / Q000254:growth & development / Q000502:physiology*; D016018:Least-Squares Analysis; D007958:Leukocyte Count; D009682:Magnetic Resonance Spectroscopy; D053858:Metabolic Networks and Pathways; D055442:Metabolome; D011498:Protein Precursors / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D011498:Protein Precursors; D002116:Calcitonin; D002097:C-Reactive Protein