title: Cytocompatibility and osteogenic activity of a novel calcium phosphate silicate bioceramic: Silicocarnotite.
abstract: In the present study, the effect of a novel bioceramic, silicon-containing calcium phosphate ceramic (silicocarnotite, Ca5 (PO4 )2 SiO4 , CPS) on attachment, proliferation, and osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (rBMSC) has been investigated in comparison to hydroxyapatite (HA). The CPS showed a similar cell attachment behavior to HA, while the proliferation of rBMSC on CPS was significantly higher than that on HA, which indicated that CPS had a good cytocompatibility. Moreover, the expression of alkaline phosphatase activity and osteogenic-related genes, including Runx-2, osteopontin (OPN), bone sialoprotein (BSP) and osteocalcin (OC), demonstrated that CPS enhanced the osteogenic differentiation of rBMSC and accelerated the differentiation process. The results suggest that CPS ceramic exhibits a good cytocompatibility and osteogenic activity, which might be used as a potential candidate material for bone tissue engineering.
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Duan
authors.forename: Congqin
authors.initials: C
authors.lastname: Ning
authors.forename: Tingting
authors.initials: T
authors.lastname: Tang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D002448:Cell Adhesion; D002454:Cell Differentiation / Q000187:drug effects; D002516:Ceramics / Q000737:chemistry*; D017886:Durapatite; D005456:Fluorescent Dyes; D007211:Indoles; D008422:Materials Testing; D059630:Mesenchymal Stem Cells; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D017640:Silicates / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D005456:Fluorescent Dyes; D007211:Indoles; D017640:Silicates; C584000:silicocarnotite; C007293:DAPI; D017886:Durapatite