title: [Research on ginsenosides composition in wild ginseng leaves in different growing years].
abstract: OBJECTIVE
  To analyze ginsenosides composition in wild ginseng leaves with different growth years.
  
  
  METHODS
  The analysis was performed on Acquity UPLC BEH Shield RP18 (2.1 mm x 100 mm, 1.7 microm) column, the mobile phase was acetonitrile-0.05% formic acid solution in gradient elution mode. The detection wavelength was at 203 nm. The flow rate was 0.4 mL x min(-1) and column temperature was set at 30 degrees C.
  
  
  RESULTS
  Thirteen ginsenosides were determined by the established UPLC method. In 5-17th growth year ginseng leaf samples cultivated simulating wild conditions, the contents of ginsenosides in the 14th year have the highest content.
  
  
  CONCLUSIONS
  The established method is simple, accurate and reliable, can be used in ginsenosides determination and fingerprint research of Panax crude drug. The result provides reliable data for the accumulation of ginsenosides and sustainable utilization of ginseng resources.
authors.affiliation: College of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, China. zhangll2@tasly.com
authors.forename: Lan-lan
authors.initials: LL
authors.lastname: Zhang
authors.forename: Wen-yuan
authors.initials: WY
authors.lastname: Gao
authors.forename: Zhao-hui
authors.initials: ZH
authors.lastname: Song
authors.forename: Nan
authors.initials: N
authors.lastname: Cai
authors.forename: Shui-ping
authors.initials: SP
authors.lastname: Zhou
mesh_terms: D004365:Drugs, Chinese Herbal / Q000032:analysis*; D036145:Ginsenosides / Q000032:analysis*; D005894:Panax / Q000737:chemistry* / Q000254:growth & development; D018515:Plant Leaves / Q000737:chemistry* / Q000254:growth & development; D013997:Time Factors
chemical_list: D004365:Drugs, Chinese Herbal; D036145:Ginsenosides