title: [Comparison of collagen fiber staining between Van-Gieson staining and Masson trichrome staining of hepatic specimens in mice with Schistosoma japonicum infection].
abstract: OBJECTIVE
  To compare the effects of collagen fiber staining between Van-Gieson staining and Masson trichrome staining of hepatic specimens in mice with Schistosoma japonicum infection.
  
  
  METHODS
  A model of hepatic granuloma and fibrosis was established by infecting mice with S. japonicum cercariae, then the hepatic specimens were taken and Van-Gieson staining and Masson trichrome staining were performed. Eventually, the area of granuloma and fibrosis were measured by imaging analysis software.
  
  
  RESULTS
  When the time of staining was 3-7 min, there was no significant difference of the fibrosis areas between the two methods (P > 0.05); when the time of staining was more than 10 min, the staining area showed by Masson's staining was significantly larger than that showed by Van-Gieson staining, and the difference was statistically significant (P < 0.05).
  
  
  CONCLUSIONS
  The operation procedures of Van-Gieson staining are simpler and easier to master than those of Masson trichrome staining, therefore Van-Gieson staining is a better method to display collagen.
authors.affiliation: Integrated Laboratory of Anhui Medical University, Hefei 230032, China.
authors.forename: Da-Ke
authors.initials: DK
authors.lastname: Huang
authors.forename: Yu-Xia
authors.initials: YX
authors.lastname: Zhang
authors.forename: Su-Qin
authors.initials: SQ
authors.lastname: Man
authors.forename: Fa-Zhi
authors.initials: FZ
authors.lastname: Yu
authors.forename: Ji-Jia
authors.initials: JJ
authors.lastname: Shen
mesh_terms: D000818:Animals; D003094:Collagen / Q000032:analysis*; D005260:Female; D008099:Liver / Q000473:pathology*; D008106:Liver Cirrhosis, Experimental / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D012554:Schistosomiasis japonica / Q000473:pathology*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D003094:Collagen