title: Simultaneous determination of spinetoram residues in tomato by high performance liquid chromatography combined with QuEChERS method.
abstract: A sensitive and simple method for simultaneous analysis of spinetoram residues and its dissipation in tomato were studied. Spinetoram residues were extracted from tomato samples with acetonitrile. The extract was cleaned-up with QuEChERS method by dispersive solid-phase extraction with primary secondary amine sorbent to remove co-extractives, prior to analysis by high performance liquid chromatography coupled with diode array detector (HPLC-DAD). This method is characterized by recovery >97 %, relative standard division (RSD) <12.3 %, and limit of quantification (LOQ) of 0.04 mg kg(-1), in agreement with directive for method validation in residue analysis. Also, the results showed that spinetoram dissipation pattern followed the first order kinetics with the half-life of 2.6 days, in tomato. The spinetoram residues in tomato were below the codex maximum residue level (0.06 mg kg(-1)) after 10 days of application. This study suggests that spinetoram is acceptable to apply for tomato under the recommended dosage.
authors.affiliation: Pesticide Residues and Environmental Pollution Department, Central Agricultural Pesticide Laboratory, Agriculture Research Center, Dokki, Giza, 12618, Egypt. farag_malhat@yahoo.com
authors.forename: Farag Mahmoud
authors.initials: FM
authors.lastname: Malhat
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006207:Half-Life; D057230:Limit of Detection; D018551:Solanum lycopersicum / Q000737:chemistry*; D018942:Macrolides / Q000032:analysis* / Q000493:pharmacokinetics; D010573:Pesticide Residues / Q000032:analysis*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction
chemical_list: D018942:Macrolides; D010573:Pesticide Residues; C548262:spinetoram