title: Human mesenchymal stem-cell behaviour on direct laser micropatterned electrospun scaffolds with hierarchical structures.
abstract: Direct laser machining and electrospinning are utilized to obtain a bi-layered hybrid scaffold with hierarchical topographical features to mimic extracellular matrix-like microenvironment of cells. Adult bone marrow derived human mesenchymal stem cells (hMSCs) are cultured in vitro in these hybrid scaffolds, and cell orientation, proliferation, viability, and differentiation are evaluated. The results show that this novel hybrid scaffold not only supports cell growth like traditional scaffolds, but also elicits positive responses from the cells, like lineage commitment and alignment, which are essential features of future scaffolds.
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore.
authors.forename: Huaqiong
authors.initials: H
authors.lastname: Li
authors.forename: Yee Shan
authors.initials: YS
authors.lastname: Wong
authors.forename: Feng
authors.initials: F
authors.lastname: Wen
authors.forename: Kee Woei
authors.initials: KW
authors.lastname: Ng
authors.forename: Gary Ka Lai
authors.initials: GK
authors.lastname: Ng
authors.forename: Subbu S
authors.initials: SS
authors.lastname: Venkatraman
authors.forename: Freddy Yin Chiang
authors.initials: FY
authors.lastname: Boey
authors.forename: Lay Poh
authors.initials: LP
authors.lastname: Tan
mesh_terms: D000293:Adolescent; D015415:Biomarkers / Q000378:metabolism; D040761:Biomimetic Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D055664:Electrochemical Techniques; D005109:Extracellular Matrix / Q000737:chemistry; D006801:Humans; D007834:Lasers; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology / Q000254:growth & development; D011091:Polyesters / Q000138:chemical synthesis* / Q000494:pharmacology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D015415:Biomarkers; D011091:Polyesters; C062968:lactide-caprolactone copolymer