title: Enzyme-catalyzed crosslinking in a partly frozen state: a new way to produce supermacroporous protein structures.
abstract: In this study a new way to produce supermacroporous protein structures was investigated. Enzyme-mediated crosslinking of gelatin or casein was performed in a partly frozen state, which yielded stable, protein-based cryogels. The reaction kinetics for the formation of cryogels were found to be fairly slow, most likely due to the low temperature (-12 °C) used or due to an increased viscosity owing to the cryo-concentration taking place. The produced cryogels were characterized with regards to their physical properties and in vitro degradation. Furthermore, cryogels produced from gelatin and casein were evaluated as potential scaffolds by fibroblast cultivation to confirm their in vitro biocompatibility. Gelatin- and casein-based scaffolds both supported cell proliferation and migration through the scaffold.
authors.affiliation: Department of Biotechnology, Lund University, P.O. Box 124, SE-22100, Lund, Sweden. harald.kirsebom@biotek.lu.se
authors.forename: Harald
authors.initials: H
authors.lastname: Kirsebom
authors.forename: Linda
authors.initials: L
authors.lastname: Elowsson
authors.forename: Dmitriy
authors.initials: D
authors.lastname: Berillo
authors.forename: Séverine
authors.initials: S
authors.lastname: Cozzi
authors.forename: Ilyas
authors.initials: I
authors.lastname: Inci
authors.forename: Erhan
authors.initials: E
authors.lastname: Piskin
authors.forename: Igor Yu
authors.initials: IY
authors.lastname: Galaev
authors.forename: Bo
authors.initials: B
authors.lastname: Mattiasson
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D002364:Caseins / Q000737:chemistry*; D002448:Cell Adhesion; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D003432:Cross-Linking Reagents / Q000737:chemistry; D059985:Cryogels / Q000138:chemical synthesis* / Q000737:chemistry; D005615:Freezing; D005780:Gelatin / Q000737:chemistry*; D007739:L Cells; D051379:Mice; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D014783:Viscosity
chemical_list: D001672:Biocompatible Materials; D002364:Caseins; D003432:Cross-Linking Reagents; D059985:Cryogels; D005780:Gelatin