title: High-throughput screening of substrate chemistry for embryonic stem cell attachment, expansion, and maintaining pluripotency.
abstract: Appropriate surface attachment is essential for growing embryonic stem (ES) cells in an undifferentiated state. It is challenging to identify the optimal surface chemistry of the substrata for ES cell attachment and maintenance. Using a rapid, high-throughput polymerization and screening platform with a comprehensive library of 66 monomer-grafted membrane surfaces, the optimal substrate, N-[3-(dimethylamino)propyl] methacrylamide (DMAPMA) has been identified to support strong attachment, high expansion capacity, and long-term self-renewal of ES cells (up to 7 passages). This monomer-based, chemically defined, scalable, sustainable, relatively inexpensive, covalently grafted, and controllable polymeric substrate provides a new opportunity to manipulate surface chemistry for pluripotent stem culture.
authors.affiliation: College of Nanoscale Science and Engineering, University at Albany, State University of New York, Albany, New York 12203, USA.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Zonca
authors.forename: Philip S
authors.initials: PS
authors.lastname: Yune
authors.forename: Caryn L
authors.initials: CL
authors.lastname: Heldt
authors.forename: Georges
authors.initials: G
authors.lastname: Belfort
authors.forename: Yubing
authors.initials: Y
authors.lastname: Xie
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques; D053595:Embryonic Stem Cells / Q000166:cytology*; D057166:High-Throughput Screening Assays / Q000379:methods*; D019012:Integrin beta1 / Q000378:metabolism; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D039904:Pluripotent Stem Cells / Q000166:cytology; D054852:Small Molecule Libraries; D013499:Surface Properties
chemical_list: D000178:Acrylamides; D019012:Integrin beta1; D054852:Small Molecule Libraries; C583028:N-(3-(dimethylamino)propyl)methacrylamide