title: Identification and assay of 3'-O-methyltaxifolin by UPLC-MS in rat plasma.
abstract: A new metabolite of taxifolin: 3'-O-methyltaxifolin (3'-O-MTAX) in Caco-2 cells and in rat plasma was identified. The chemical structure of 3'-O-MTAX was determined by MS and (1)H NMR. A rapid, sensitive and specific UPLC-MS method to determine 3'-O-MTAX in rat plasma was also developed. Following ethyl acetate extraction, 3'-O-MTAX in plasma was separated on a Sunfire™ (2.1mm×50mm, 3.5μm) column and analyzed in the selected ion recording with a negative electrospray ionization mode using puerarin as the internal standard. The lower limit of quantification (LLOQ) was 2.75ng/mL. Intra- and inter-day precisions (% RSD) were all within 7.2% and accuracy (% deviation) ranged from -5.0 to 4.7%. The overall recoveries at four concentrations were all >72.0%. This validated method was successfully applied to measure 3'-O-MTAX in rat plasma after oral administration of taxifolin.
authors.affiliation: The Affiliated Wuxi Second Hospital, Nanjing Medical University, Wuxi 214002, Jiangsu Province, PR China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.forename: Su
authors.initials: S
authors.lastname: Zeng
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D007529:Isoflavones / Q000097:blood / Q000737:chemistry; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D007529:Isoflavones; D011794:Quercetin; C003377:taxifolin; C033607:puerarin