title: [Synovialitis of the arthrofibrotic type: criteria of a new synovialitis type for the diagnosis of arthrofibrosis].
abstract: After rheumatologic conservative medical therapy has been exhausted in degenerative and inflammatory joint diseases, arthroplastic operations are an important option to restore quality of life. Endoprosthesis-associated arthrofibrosis is a severe fibrosing disease of the synovial membrane after endoprosthetic operations. Neither the morphological substrate nor histopathological criteria have been described. The aim was to describe the histopathological substrate of arthrofibrosis and to define histological and immunohistochemical criteria of arthrofibrosis on the basis of tissue samples derived from revision. In histopathological analyses arthrofibrosis revealed a synovialitis with varying fibrosis, without detectable ossification and without minimal wear particle reaction (so-called synovialitis of arthrofibrotic type, SAT). A 3-stage grading was determined based on the cellular density of the fibrous tissue (fibroblast cellularity). In 191 cases with SAT, grade 1 was found in 24.1 % (n = 46), grade 2 was found in 51.8 % (n = 99) and grade 3 was found in 24.1 % (n = 46). The control group consisted of 29 cases with synovialitis of indifferent type (type IV membrane). If SAT grades 2 and 3 are summed together, i.e. the distance between the fibroblasts was less than two cell lengths, the difference of the fibroblast cellularity compared with the type IV membrane was significant (p < 0.001). Above SAT grade 2 the diagnosis of arthrofibrosis could be made with a sensitivity 0.7592 and specificity 0.8276. The SM-alpha-actin cytoplasmic positivity of fibroblasts indicates a myofibroblast phenotype and the β-catenin positivity suggests a resemblance to fibromatosis or a keloid-like process. In the quantitative evaluation of the β-catenin positive fibroblasts, there was a significant difference (p < 0.001) between type IV membrane and SAT. A threshold value of 20 beta-catenin positive cells per microscopic high power field (HPF) was determined, which represents in conjunction with the clinical information a new histopathological diagnosis component (sensitivity 0.720, specificity 0.867).
authors.affiliation: Zentrum für Histologie, Zytologie und Molekulare Diagnostik, Max-Planck-Str. 18+20, 54296 Trier, Deutschland. krenn@patho-trier.de
authors.forename: V
authors.initials: V
authors.lastname: Krenn
authors.forename: M
authors.initials: M
authors.lastname: Ruppert
authors.forename: P
authors.initials: P
authors.lastname: Knöß
authors.forename: D
authors.initials: D
authors.lastname: Kendoff
authors.forename: C
authors.initials: C
authors.lastname: Poremba
authors.forename: M
authors.initials: M
authors.lastname: Thomsen
authors.forename: M
authors.initials: M
authors.lastname: Skutek
authors.forename: J
authors.initials: J
authors.lastname: Hassenpflug
authors.forename: R
authors.initials: R
authors.lastname: Ascherl
authors.forename: M G
authors.initials: MG
authors.lastname: Krukemeyer
authors.forename: G
authors.initials: G
authors.lastname: Matziolis
authors.forename: P
authors.initials: P
authors.lastname: Thomas
authors.forename: T
authors.initials: T
authors.lastname: Gehrke
mesh_terms: D000368:Aged; D001172:Arthritis, Rheumatoid / Q000150:complications* / Q000601:surgery*; D003937:Diagnosis, Differential; D005260:Female; D005355:Fibrosis / Q000209:etiology / Q000473:pathology; D006801:Humans; D007595:Joint Prosthesis / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D013585:Synovitis / Q000209:etiology* / Q000473:pathology*; D009626:Terminology as Topic