title: Relative oral bioavailability of 3-MCPD from 3-MCPD fatty acid esters in rats.
abstract: In order to quantify the relative oral bioavailability of 3-chloropropane-1,2-diol (3-MCPD) from 3-MCPD fatty acid diesters in vivo, 1,2-dipalmitoyl-3-chloropropane-1,2-diol (3-MCPD diester) and 3-MCPD were orally applied to rats in equimolar doses. In both cases, the time courses of 3-MCPD concentrations were measured in blood, various organs, tissues and intestinal luminal contents. The results show that 3-MCPD is released by enzymatic hydrolysis from the 3-MCPD diester in the gastrointestinal tract and distributed to blood, organs and tissues. Based on the measurements in blood, the areas under the curve (AUC) for 3-MCPD were calculated. By comparing both AUC, the relative amount of 3-MCPD bioavailable from the 3-MCPD diester was calculated to be 86 % on average of the amount bioavailable following administration of 3-MCPD. In view of limited experimental data, it is justified for the purpose of risk assessment to assume complete hydrolysis of the diesters in the gastro-intestinal tract. Therefore, assessment of the extent of exposure to 3-MCPD released from its fatty acid esters should be performed in the same way as exposure to the same molar quantity of 3-MCPD.
authors.affiliation: Department of Food Safety, Federal Institute for Risk Assessment, Max-Dohrn-Strasse 8-10, 10589 Berlin, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Abraham
authors.forename: Klaus E
authors.initials: KE
authors.lastname: Appel
authors.forename: Edith
authors.initials: E
authors.lastname: Berger-Preiss
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Apel
authors.forename: Susanne
authors.initials: S
authors.lastname: Gerling
authors.forename: Hans
authors.initials: H
authors.lastname: Mielke
authors.forename: Otto
authors.initials: O
authors.lastname: Creutzenberg
authors.forename: Alfonso
authors.initials: A
authors.lastname: Lampen
mesh_terms: D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D001682:Biological Availability; D002273:Carcinogens / Q000737:chemistry / Q000493:pharmacokinetics*; D004952:Esters / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D005506:Food Contamination / Q000032:analysis; D005766:Gastrointestinal Contents / Q000737:chemistry; D005990:Glycerol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics; D006868:Hydrolysis; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D018570:Risk Assessment; D000517:alpha-Chlorohydrin
chemical_list: D002273:Carcinogens; D004952:Esters; D005227:Fatty Acids; D000517:alpha-Chlorohydrin; D005990:Glycerol