title: Triple-stranded DNA containing 8-oxo-7,8-dihydro-2'-deoxyguanosine: implication in the design of selective aptamer sensors for 8-oxo-7,8-dihydroguanine.
abstract: 8-Oxo-7,8-dihydroguanine (8-oxoG, or OG) as a free base has been widely considered as a biomarker for DNA oxidative damage. Currently no fluorescence sensor has been developed to directly detect 8-oxoG less than 100 nM. In this study, two triple-stranded DNAs were selected as the scaffolds to rationally design DNA aptamer sensors for 8-oxoG. The cavity was created by deleting the 8-oxodG nucleoside in a triplex containing an A·OG-C triad or a C·OG-A triad. The results showed that the fluorescence of both sensors were completely quenched by 8-oxoG. The detection ranges of the two sensors were different, while the combined range was comparable to the detection range of an antibody-based method. This result is expected to enable a fast, low-cost, and reusable method to measure 8-oxoG concentration.
authors.affiliation: Department of Chemistry and Environmental Science, New Jersey Institute of Technology, Newark, 07102, United States.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yiqi
authors.initials: Y
authors.lastname: Wang
authors.forename: Xianyang
authors.initials: X
authors.lastname: Meng
authors.forename: Rik
authors.initials: R
authors.lastname: Dhar
authors.forename: Haidong
authors.initials: H
authors.lastname: Huang
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis; D005456:Fluorescent Dyes / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D044366:Transition Temperature; D014466:Ultraviolet Rays
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine