title: Molecular cloning, tissue expression, and analysis with genome DNA methylation of porcine LSD1 gene.
abstract: Lysine-specific demethylase 1 (LSD1) functioned as a demethyl methylase gene, underlying a wide range of biological processes, including cancer, cell apoptosis, differentiation, and development. To further understand the functions of the porcine LSD1 gene, we first obtained cDNA sequence of porcine LSD1 gene, using in silico cloning method. We further found that the porcine LSD1 gene has two transcripts, in which cDNA sequences are 2,716 and 2,656 bp, ORF are 2,622 and 2,562 bp, respectively. Then, RT-PCR analysis showed that the LSD1 gene is expressed in various tissues and relatively higher in the tissues of ovary, kidney, and spleen. Besides, the LSD1 gene was expressed higher in the growth nonage and peaked at 3 days in muscle tissue. Meanwhile, the expression of two transcript variants of LSD1 gene presented the same change trend. Besides, the level of DNA methylation was approximately fourfold higher in a 3-day muscle than in an old pig (180 days), significantly positive related to the gene expression of LSD1 (R = 0.9362, P < 0.05), and declined with growing age. Cloning, expression pattern, and analysis with genome DNA methylation of porcine LSD1 gene laid a foundation to clarify the molecular mechanisms of porcine growth and development and also for further work on animal breeding.
authors.affiliation: Agricultural Ministry Key Laboratory of Swine Breeding and Genetics & Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, 430070 Wuhan, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Chai
authors.forename: Lina
authors.initials: L
authors.lastname: Liu
authors.forename: Qi
authors.initials: Q
authors.lastname: Xiong
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Yongdong
authors.initials: Y
authors.lastname: Peng
authors.forename: Wei
authors.initials: W
authors.lastname: Jin
authors.forename: Rong
authors.initials: R
authors.lastname: Zheng
authors.forename: Jian
authors.initials: J
authors.lastname: Peng
authors.forename: Siwen
authors.initials: S
authors.lastname: Jiang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D019175:DNA Methylation; D016678:Genome; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009928:Organ Specificity; D010089:Oxidoreductases, N-Demethylating / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D013552:Swine / Q000235:genetics* / Q000378:metabolism; D014714:Vertebrates / Q000145:classification / Q000235:genetics / Q000378:metabolism
chemical_list: D010089:Oxidoreductases, N-Demethylating