title: Sphingolipid content in the human uterus and pair-matched uterine leiomyomas remains constant.
abstract: In the present work we sought to investigate the content of sphingolipids (sphingosine, sphinganine, sphingosine-1-phosphate and ceramide) in human fibroids and pair-matched healthy uterus tissue. We demonstrated that, in uterine leiomyomas, the contents of  sphingosine, sphinganine, sphingosine-1-phosphate and ceramide remains quite constant. However, a trend towards elevation of ceramide and simultaneous reduction of sphingosine-1 phosphate levels was also noticed. Additionally, in uterine leiomyomas we found relevant activation of both PTEN and MAPK(ERK1/2) signaling pathways with only a minor change in AKT activity and relatively absent HIF-1α/AMPK activation. In conclusion, rather modest changes in sphingolipids are correlated with the activation of PTEN and MAPK(ERK1/2) signaling proteins in human uterine leiomyomas.
authors.affiliation: Department of Gynecology and Gynecological Oncology, Medical University of Bialystok, 24a Skłodowskiej Str., 15-269 Białystok, Poland.
authors.forename: Knapp
authors.initials: K
authors.lastname: Paweł
authors.forename: Adrian
authors.initials: A
authors.lastname: Chabowski
authors.forename: Jan
authors.initials: J
authors.lastname: Górski
mesh_terms: D000328:Adult; D002518:Ceramides / Q000032:analysis / Q000378:metabolism; D004789:Enzyme Activation; D005260:Female; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D007889:Leiomyoma / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D020935:MAP Kinase Signaling System; D008875:Middle Aged; D051059:PTEN Phosphohydrolase / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D013107:Sphingolipids / Q000032:analysis* / Q000378:metabolism*; D014594:Uterine Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D014599:Uterus / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology*
chemical_list: D002518:Ceramides; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D013107:Sphingolipids; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase