title: Chiral determination of a novel acaricide cyflumetofen enantiomers in cucumber, tomato, and apple by HPLC.
abstract: A simple chiral analytical method was developed for the enantiomeric determination of cyflumetofen in cucumber, tomato, and apple by normal-phase HPLC. The effects of mobile phase composition and column temperature on the enantioseparation were evaluated. Excellent separation was achieved at 25°C on a Chiralpak AD-H column, with a mixture of n-hexane and 2-propanol (95:5, v/v) as mobile phase at a flow rate of 1.0 mL/min detecting at 234 nm. The resolution of cyflumetofen enantiomers was up to 5.5. The elution order of the enantiomers was determined by an online OR-2090 detector, which was performed under the same chromatographic conditions. The first eluted enantiomer was (-)-cyflumetofen and the second eluted one was (+)-cyflumetofen. The method was validated for linearity, repeatability, accuracy, LOD, and LOQ. LOD ranged from 0.1 to 0.15 mg/kg, with the LOD varying from 0.33 to 0.5 mg/kg for each enantiomer, respectively. The average recoveries of the pesticide ranged from 71.4 to 102.0% at all fortification levels. The precision values associated with the analytical method, expressed as RSD values, were below 14.8% in all matrices. The method was then successfully applied to detect cyflumetofen enantiomers in real samples.
authors.affiliation: Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Key Laboratory of Pesticide Chemistry and Application Ministry of Agriculture, Beijing, P R China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Chen
authors.forename: Fengshou
authors.initials: F
authors.lastname: Dong
authors.forename: Xingang
authors.initials: X
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Yuanbo
authors.initials: Y
authors.lastname: Li
authors.forename: Minmin
authors.initials: M
authors.lastname: Li
authors.forename: Yunhao
authors.initials: Y
authors.lastname: Wang
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D056810:Acaricides / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D018553:Cucumis sativus / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis; D018551:Solanum lycopersicum / Q000737:chemistry*; D027845:Malus / Q000737:chemistry*; D011422:Propionates / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D056810:Acaricides; D011422:Propionates; C572153:cyflumetofen