title: The protective effect of menhaden oil in the oxidative damage and renal necrosis due to dietary choline deficiency.
abstract: Weanling rats fed a choline-deficient diet develop kidney oxidative damage, tubular and cortical kidney necrosis, renal failure and animal death. The effect of dietary menhaden oil was assayed on the mentioned sequence correlating oxidative stress with renal structure and function. Rats were fed ad libitum 4 different diets: (a) a choline-deficient diet with corn oil and sunflower hydrogenated oil as a source of fatty acids; (b) the same diet supplemented with choline; (c) a choline-deficient diet with menhaden oil as a source of fatty acids; and (d) the previous diet supplemented with choline. Animals were sacrificed at days 0, 2, 4 and 7. The histopathological study of the kidneys showed that renal necrosis was only observed at day 7 in choline-deficient rats receiving the vegetable oil diet, simultaneously with increased creatinine plasma levels. Homogenate chemiluminescence (BOOH-initiated chemiluminescence) and phospholipid oxidation indicate the development of oxidative stress and damage in choline-deficient rats fed vegetable oils as well as the protective effect of menhaden oil. Rats fed with the fish oil diet showed that oxidative stress and damage develop later, as compared with vegetable oil, with no morphological damage during the experimental period.
authors.affiliation: Centre of Experimental Pathology, Faculty of Medicine, University of Buenos Aires, JE Uriburu 950, Buenos Aires, Argentina. georginaossani@gmail.com
authors.forename: Georgina P
authors.initials: GP
authors.lastname: Ossani
authors.forename: Marisa G
authors.initials: MG
authors.lastname: Repetto
authors.forename: Alberto
authors.initials: A
authors.lastname: Boveris
authors.forename: Alberto J
authors.initials: AJ
authors.lastname: Monserrat
mesh_terms: D000208:Acute Disease; D000818:Animals; D002794:Choline / Q000008:administration & dosage*; D002796:Choline Deficiency / Q000150:complications / Q000188:drug therapy* / Q000473:pathology; D003314:Corn Oil / Q000008:administration & dosage; D003404:Creatinine / Q000097:blood; D004032:Diet; D019587:Dietary Supplements; D005395:Fish Oils / Q000008:administration & dosage*; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D007674:Kidney Diseases / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D008297:Male; D009336:Necrosis; D018384:Oxidative Stress / Q000187:drug effects*; D010743:Phospholipids / Q000378:metabolism; D010938:Plant Oils / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D000074242:Sunflower Oil
chemical_list: D005395:Fish Oils; D010743:Phospholipids; D010938:Plant Oils; D000074242:Sunflower Oil; C033266:Menhaden oil; D003314:Corn Oil; D003404:Creatinine; D002794:Choline