title: Expression of myelin and lymphocyte protein (MAL) in oral carcinogenesis.
abstract: In the pathological diagnosis of oral squamous cell carcinoma, we often confront the difficulty of determining whether it is invasive carcinoma or epithelial dysplasia. Recently, myelin and lymphocyte protein (MAL; T-cell differentiation-related gene) has been reported to be a candidate gene suppressed in esophageal carcinoma. When we performed cDNA microarray analysis, we found that gene expression of MAL was significantly downregulated in oral squamous cell carcinoma (OSCC). We evaluated the expression of the MAL gene by laser microdissection and real-time PCR methods and protein localization by immunohistochemistry. The gene expression of MAL was significantly decreased in OSCC compared with normal epithelium (P < 0.05). Furthermore, protein expression of MAL disappeared gradually in proportion to malignancy. The results suggest that MAL plays an important role during oral carcinogenesis and that the gene may have potential as a biomarker target for OSCC.
authors.affiliation: Department of Oral Pathology and Diagnosis, School of Dentistry, Showa University, 1-5-8, Hatanodai, Tokyo 142-8555, Japan.
authors.forename: Samir Kumar
authors.initials: SK
authors.lastname: Pal
authors.forename: Sunaki
authors.initials: S
authors.lastname: Noguchi
authors.forename: Gou
authors.initials: G
authors.lastname: Yamamoto
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamada
authors.forename: Tomohide
authors.initials: T
authors.lastname: Isobe
authors.forename: Shigeo
authors.initials: S
authors.lastname: Hayashi
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Tanaka
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Ryutaro
authors.initials: R
authors.lastname: Kamijo
authors.forename: Gen-Yuki
authors.initials: GY
authors.lastname: Yamane
authors.forename: Tetsuhiko
authors.initials: T
authors.lastname: Tachikawa
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D004847:Epithelial Cells / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D062806:Myelin and Lymphocyte-Associated Proteolipid Proteins / Q000235:genetics* / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D013447:Sulfites / Q000737:chemistry
chemical_list: D014408:Biomarkers, Tumor; C569026:MAL protein, human; D062806:Myelin and Lymphocyte-Associated Proteolipid Proteins; D013447:Sulfites; C042345:hydrogen sulfite