title: Development of a fast and sensitive glucose biosensor using iridium complex-doped electrospun optical fibrous membrane.
abstract: Polystyrene electrospun optical fibrous membrane (EOF) was fabricated using a one-step electrospinning technique, functionalized with glucose oxidases (GOD/EOF), and used as a quick and highly sensitive optical biosensor. Because of the doped iridium complex, the fibrous membrane emitted yellow luminescence (562 nm) when excited at 405 nm. Its luminescence was significantly enhanced with the presence of extremely low concentration glucose. The detection limit was of 1.0 × 10(-10) M (S/N = 3), superior to that of reported glucose biosensor with 1.2 × 10(-10) M. A linear range between the relative intensity increase and the logarithm of glucose concentration was exhibited from 3.0 × 10(-10) M to 1.3 × 10(-4) M, which was much wider than reported results. Notably, the response time was less than 1 s. These high sensitivity and fast response were attributed to the high surface-area-to-volume of the porous fibrous membrane, the efficient GOD biocatalyst reaction on the fibers surface, as well as the fast electron or energy transfer between dissolved oxygen and the optical fibrous membrane.
authors.affiliation: Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu, 610064, PR China. zcs@scu.edu.cn
authors.forename: Cuisong
authors.initials: C
authors.lastname: Zhou
authors.forename: Yalin
authors.initials: Y
authors.lastname: Shi
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Ding
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Luo
authors.forename: Zhiyun
authors.initials: Z
authors.lastname: Lu
authors.forename: Dan
authors.initials: D
authors.lastname: Xiao
mesh_terms: D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D005336:Fiber Optic Technology; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007495:Iridium / Q000737:chemistry*; D008163:Luminescent Measurements; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry*; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry; D013499:Surface Properties
chemical_list: D001786:Blood Glucose; D009942:Organometallic Compounds; D011137:Polystyrenes; D007495:Iridium; D005949:Glucose Oxidase