title: [Effect of gas-turbine green discoloring and drying processing methods on herbal quality of tetraploid Lonicerae Japonicae Flos].
abstract: OBJECTIVE
  To study the effect of gas-turbine green discoloring and drying processing method on the quality of various Lonicerae Japonicae Flos herbs.
  
  
  METHODS
  DIKMA DiamonsilTM-C18 column (4.6 mm x 250 mm, 5 microm) was adopted using HPLC Waters 1525 and eluted with acetonitrile and 0.1% phosphate acid as the mobile phase. The flow rate was 1.0 mL x min(-1) , the column temperature was 25 degrees C the detection wavelength was 355 nm.
  
  
  RESULTS
  After being processed by the gas-turbine green discoloring and drying method, tetraploid Lonicerae Japonicae Flos showed a green color. The contents of chlorogenic acid and galuteolin were 5.31% and 0.105% , both significantly higher by 18.0% and 32.1% than those of diploid Lonicerae Japonicae Flos processed by the same method. The content of chlorogenic acid in tetraploid Lonicerae Japonicae Flos processed the gas-turbine green discoloring and drying method were also remarkably higher than that of tetraploid and diploid Lonicerae Japonicae Flos processed by traditional processing method of natural drying.
  
  
  CONCLUSIONS
  The gas-turbine green discoloring and drying processing method is a new-type drying method suitable for tetraploid Lonicerae Japonicae Flos. Under the condition of gas-turbine green discoloring and drying processing, tetraploid Lonicerae Japonicae Flos shows much higher quality than Lonicerae Japonicae Flos, suggesting that it is a good variety worth popularizing and applying.
authors.affiliation: School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Hu
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Li
authors.forename: Ou
authors.initials: O
authors.lastname: Li
authors.forename: Jiang-bo
authors.initials: JB
authors.lastname: Hao
authors.forename: Jia-kun
authors.initials: JK
authors.lastname: Liu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D035264:Flowers / Q000737:chemistry* / Q000235:genetics; D006358:Hot Temperature; D031239:Lonicera / Q000737:chemistry* / Q000235:genetics; D011786:Quality Control; D013678:Technology, Pharmaceutical / Q000379:methods*; D057891:Tetraploidy
chemical_list: D004365:Drugs, Chinese Herbal