title: Fast parallel detection of feline panleukopenia virus DNA by multi-channel microchip electrophoresis with programmed step electric field strength.
abstract: A multi-channel microchip electrophoresis using a programmed step electric field strength (PSEFS) method was investigated for fast parallel detection of feline panleukopenia virus (FPV) DNA. An expanded laser beam, a 10× objective lens, and a charge-coupled device camera were used to simultaneously detect the separations in three parallel channels using laser-induced fluorescence detection. The parallel separations of a 100-bp DNA ladder were demonstrated on the system using a sieving gel matrix of 0.5% poly(ethylene oxide) (M(r)  = 8 000 000) in the individual channels. In addition, the PSEFS method was also applied for faster DNA separation without loss of resolving power. A DNA size marker, FPV DNA sample, and a negative control were simultaneously analyzed with single-run and one-step detection. The FPV DNA was clearly distinguished within 30 s, which was more than 100 times faster than with conventional slab gel electrophoresis. The proposed multi-channel microchip electrophoresis with PSEFS was demonstrated to be a simple and powerful diagnostic method to analyze multiple disease-related DNA fragments in parallel with high speed, throughput, and accuracy.
authors.affiliation: Department of Applied Chemistry, College of Applied Science, Kyung Hee University, Yongin-si, Gyeonggi-do, Republic of Korea.
authors.forename: He
authors.initials: H
authors.lastname: Nan
authors.forename: Dong Jin
authors.initials: DJ
authors.lastname: Yoo
authors.forename: Seong Ho
authors.initials: SH
authors.lastname: Kang
mesh_terms: D000818:Animals; D002415:Cats; D004279:DNA, Viral / Q000737:chemistry*; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D005254:Feline Panleukopenia / Q000175:diagnosis / Q000821:virology; D028301:Feline Panleukopenia Virus / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D004279:DNA, Viral