title: [Effects of temperature and pH on digestive enzymes activities in Whitmania pigra].
abstract: OBJECTIVE
  Current study was conducted to investigate and compare the impact of temperature and pH on the activities of amylase, protease and lipase in alimentary tract of Whitmania pigra.
  
  
  METHODS
  The responses of amylase, protease, and lipase activities were determined over a wide range of temperatures (7-52 degrees C) and pH gradient (2.2-11.2).
  
  
  RESULTS
  The highest lipase activity was found under 37 degrees C, pH 8.2, and the highest amylase activity was detected under 37 degrees C, pH 5.2, while protease activity peaked at 42 degrees C, pH 3.2 or pH 9.2.
  
  
  CONCLUSIONS
  The optimal temperature in alimentary tract of Wh. pigra for lipase and amylase was 37 degrees C, and the responding temperature for protease was 42 degrees C. The optimal pH value in alimentary tract of Wh. pigra for lipase and amylase was pH 8.2 and pH 5.2, respectively. While pH 3.2 or 9.2 seems to be both favorable for high protease activity.
authors.affiliation: Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Hong-zhuan
authors.initials: HZ
authors.lastname: Shi
authors.forename: Qiao-sheng
authors.initials: QS
authors.lastname: Guo
authors.forename: Zai-biao
authors.initials: ZB
authors.lastname: Zhu
authors.forename: Ting-ting
authors.initials: TT
authors.lastname: Hu
authors.forename: Shuai-wen
authors.initials: SW
authors.lastname: Zhang
mesh_terms: D000681:Amylases / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D004064:Digestive System / Q000737:chemistry / Q000201:enzymology*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007865:Leeches / Q000737:chemistry / Q000201:enzymology*; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism; D010447:Peptide Hydrolases / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature
chemical_list: D008049:Lipase; D000681:Amylases; D010447:Peptide Hydrolases