title: Dual instrument for in vivo and ex vivo OCT imaging in an ENT department.
abstract: A dual instrument is assembled to investigate the usefulness of optical coherence tomography (OCT) imaging in an ear, nose and throat (ENT) department. Instrument 1 is dedicated to in vivo laryngeal investigation, based on an endoscope probe head assembled by compounding a miniature transversal flying spot scanning probe with a commercial fiber bundle endoscope. This dual probe head is used to implement a dual channel nasolaryngeal endoscopy-OCT system. The two probe heads are used to provide simultaneously OCT cross section images and en face fiber bundle endoscopic images. Instrument 2 is dedicated to either in vivo imaging of accessible surface skin and mucosal lesions of the scalp, face, neck and oral cavity or ex vivo imaging of the same excised tissues, based on a single OCT channel. This uses a better interface optics in a hand held probe. The two instruments share sequentially, the swept source at 1300 nm, the photo-detector unit and the imaging PC. An aiming red laser is permanently connected to the two instruments. This projects visible light collinearly with the 1300 nm beam and allows pixel correspondence between the en face endoscopy image and the cross section OCT image in Instrument 1, as well as surface guidance in Instrument 2 for the operator. The dual channel instrument was initially tested on phantom models and then on patients with suspect laryngeal lesions in a busy ENT practice. This feasibility study demonstrates the OCT potential of the dual imaging instrument as a useful tool in the testing and translation of OCT technology from the lab to the clinic. Instrument 1 is under investigation as a possible endoscopic screening tool for early laryngeal cancer. Larger size and better quality cross-section OCT images produced by Instrument 2 provide a reference base for comparison and continuing research on imaging freshly excised tissue, as well as in vivo interrogation of more superficial skin and mucosal lesions in the head and neck patient.
authors.affiliation: Applied Optics Group, School of Physical Sciences, University of Kent, CT2 7NH Canterbury,  UK .
authors.forename: Ramona
authors.initials: R
authors.lastname: Cernat
authors.forename: Taran S
authors.initials: TS
authors.lastname: Tatla
authors.forename: Jingyin
authors.initials: J
authors.lastname: Pang
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Tadrous
authors.forename: Adrian
authors.initials: A
authors.lastname: Bradu
authors.forename: George
authors.initials: G
authors.lastname: Dobre
authors.forename: Grigory
authors.initials: G
authors.lastname: Gelikonov
authors.forename: Valentin
authors.initials: V
authors.lastname: Gelikonov
authors.forename: Adrian Gh
authors.initials: AG
authors.lastname: Podoleanu