title: Modification of human cancellous bone using Thai silk fibroin and gelatin for enhanced osteoconductive potential.
abstract: The modification of human cancellous bone (hBONE) with silk fibroin/gelatin (SF/G) using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC)/N-hydroxysuccini-mide (NHS) crosslinking was established. The SF/G solutions at a weight ratio of 50/50 and the solution concentrations of 1, 2, and 4 wt % were studied. SF/G sub-matrix was formed on the surface and inside pore structure of hBONE. All hBONE scaffolds modified with SF/G showed smaller pore sizes, less porosity, and slightly lower compressive modulus than unmodified hBONE. SF/G sub-matrix was gradually biodegraded in collagenase solution along 4 days. The hBONE scaffolds modified with SF/G, particularly at 2 and 4 wt % solution concentrations, promoted attachment, proliferation, and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (MSC), comparing to the original hBONE. The highest cell number, ALP activity and calcium production were observed for MSC cultured on the hBONE scaffolds modified with 4 wt % SF/G. The mineralization was also remarkably induced in the cases of modified hBONE scaffolds as observed from the deposited calcium phosphate by EDS. The modification of hBONE with SF/G was, therefore, the promising method to enhance the osteoconductive potential of human bone graft for bone tissue engineering.
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Phatumwan, Bangkok, Thailand.
authors.forename: Rungnapa
authors.initials: R
authors.lastname: Vorrapakdee
authors.forename: Sorada
authors.initials: S
authors.lastname: Kanokpanont
authors.forename: Juthamas
authors.initials: J
authors.lastname: Ratanavaraporn
authors.forename: Saranatra
authors.initials: S
authors.lastname: Waikakul
authors.forename: Chris
authors.initials: C
authors.lastname: Charoenlap
authors.forename: Siriporn
authors.initials: S
authors.lastname: Damrongsakkul
mesh_terms: D018786:Bone Substitutes; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D005349:Fibroins; D005780:Gelatin; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology; D047011:Silk / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission
chemical_list: D018786:Bone Substitutes; D047011:Silk; D005780:Gelatin; D005349:Fibroins