title: Determination of orbifloxacin in sheep plasma by high performance liquid chromatography with ultraviolet detection after intravenous and intramuscular administration.
abstract: BACKGROUND
  Single-dose pharmacokinetics of orbifloxacin (2.5mg/kg body weight) were determined in clinically normal female Patanwadi sheep (n=6) following intravenous and intramuscular administration.
  
  
  METHODS
  Orbifloxacin concentrations were determined by high performance liquid chromatography with ultraviolet detection. The concentration-time data were analyzed by non-compartmental kinetic method.
  
  
  CONCLUSIONS
  Following a single intravenous injection, an elimination half-life (t(1/2β)) of 8.31±0.102h. Steady-state volume of distribution (Vd(ss)) and total body clearance (Cl(B)) were 3.09±0.282L/kg and 0.158±0.006L/kg/h, respectively. Following intramuscular administration, an elimination rate constant (β), the area under the curve from zero to infinity (AUC(0-∞)) and the mean absorption time (MAT) were 0.015±0.001h(-1), 23.49±1.722μg·h/mL and 7.50±0.58h, respectively. The peak plasma concentration (C(max)) of 1.81±0.005µg/mL was achieved at 1.00±0.00h. The mean residence time (MRT) was 26.25±1.083h and the absolute bioavailability was 150.8±12.35%, respectively. Orbifloxacin could be useful for the treatment of bacterial infections in sheep that are sensitive to this drug.
authors.affiliation: Department of Pharmacology & Toxicology, College of Veterinary Science & Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar-385506, Gujarat, India. drgvets@gmail.com
authors.forename: G B
authors.initials: GB
authors.lastname: Dudhatra
authors.forename: Avinash
authors.initials: A
authors.lastname: Kumar
authors.forename: M M
authors.initials: MM
authors.lastname: Awale
authors.forename: D R
authors.initials: DR
authors.lastname: Kamani
authors.forename: B N
authors.initials: BN
authors.lastname: Chauhan
authors.forename: H B
authors.initials: HB
authors.lastname: Patel
authors.forename: S K
authors.initials: SK
authors.lastname: Mody
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002939:Ciprofloxacin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood; D005260:Female; D007273:Injections, Intramuscular; D012756:Sheep / Q000097:blood*; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D002939:Ciprofloxacin; C095163:orbifloxacin