title: Tumor cell differentiation by label-free fluorescence microscopy.
abstract: Autofluorescence spectra, images, and decay kinetics of U251-MG glioblastoma cells prior and subsequent to activation of tumor suppressor genes are compared. While phase contrast images and fluorescence intensity patterns of tumor (control) cells and less malignant cells are similar, differences can be deduced from autofluorescence spectra and decay kinetics. In particular, upon near UV excitation, the fluorescence ratio of the free and protein-bound coenzyme nicotinamid adenine dinucleotide depends on the state of malignancy and reflects different cytoplasmic (including lysosomal) and mitochondrial contributions. While larger numbers of fluorescence spectra are evaluated by principal component analysis, a multivariate data analysis method, additional information on cell metabolism is obtained from spectral imaging and fluorescence lifetime imaging microscopy.
authors.affiliation: Institut für Angewandte Forschung, Hochschule Aalen, Beethovenstr. 1, 73430 Aalen, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Weber
authors.forename: Michael
authors.initials: M
authors.lastname: Wagner
authors.forename: Petra
authors.initials: P
authors.lastname: Kioschis
authors.forename: Waltraud
authors.initials: W
authors.lastname: Kessler
authors.forename: Herbert
authors.initials: H
authors.lastname: Schneckenburger
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D003999:Dichloroacetic Acid / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000378:metabolism; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D025341:Principal Component Analysis; D012402:Rotenone / Q000494:pharmacology; D013050:Spectrometry, Fluorescence / Q000379:methods*; D015533:Transcriptional Activation / Q000187:drug effects; D025521:Tumor Suppressor Proteins / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D025521:Tumor Suppressor Proteins; D012402:Rotenone; D003999:Dichloroacetic Acid