title: Optical coherence tomography for live phenotypic analysis of embryonic ocular structures in mouse models.
abstract: Mouse models of ocular diseases provide a powerful resource for exploration of molecular regulation of eye development and pre-clinical studies. Availability of a live high-resolution imaging method for mouse embryonic eyes would significantly enhance longitudinal analyses and high-throughput morphological screening. We demonstrate that optical coherence tomography (OCT) can be used for live embryonic ocular imaging throughout gestation. At all studied stages, the whole eye is within the imaging distance of the system and there is a good optical contrast between the structures. We also performed OCT eye imaging in the embryonic retinoblastoma mouse model Pax6-SV40 T-antigen, which spontaneously forms lens and retinal lesions, and demonstrate that OCT allows us to clearly differentiate between the mutant and wild type phenotypes. These results demonstrate that OCTin utero imaging is a potentially useful tool to study embryonic ocular diseases in mouse models.
authors.affiliation: Baylor College of Medicine, Department of Molecular Physiology and Biophysics, One Baylor Plaza, Houston, TX 77030, USA. larina@bcm.tmc.edu
authors.forename: Irina V
authors.initials: IV
authors.lastname: Larina
authors.forename: Saba H
authors.initials: SH
authors.lastname: Syed
authors.forename: Narendran
authors.initials: N
authors.lastname: Sudheendran
authors.forename: Paul A
authors.initials: PA
authors.lastname: Overbeek
authors.forename: Mary E
authors.initials: ME
authors.lastname: Dickinson
authors.forename: Kirill V
authors.initials: KV
authors.lastname: Larin
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D010641:Phenotype; D011296:Prenatal Diagnosis / Q000295:instrumentation; D015203:Reproducibility of Results; D019572:Retinal Neoplasms / Q000196:embryology* / Q000473:pathology*; D012175:Retinoblastoma / Q000196:embryology* / Q000473:pathology*; D042261:Retinoscopes; D012680:Sensitivity and Specificity; D041623:Tomography, Optical Coherence / Q000295:instrumentation*