title: Effect of human parathyroid hormone on hematopoietic progenitor cells in NOD/SCID mice co-transplanted with human cord blood mononuclear cells and mesenchymal stem cells.
abstract: OBJECTIVE
  We evaluated the effect of human parathyroid hormone (hPTH) on the engraftment and/or in vivo expansion of hematopoietic stem cells in an umbilical cord blood (UCB)-xenotransplantation model. In addition, we assessed its effect on the expression of cell adhesion molecules.
  
  
  METHODS
  Female NOD/SCID mice received sublethal total body irradiation with a single dose of 250 cGy. Eighteen to 24 hours after irradiation, 1 × 10(7) human UCB-derived mononuclear cells (MNCs) and 5 × 10(6) human UCB-derived mesenchymal stem cells (MSCs) were infused via the tail vein. Mice were randomly divided into three groups: Group 1 mice received MNCs only, Group 2 received MNCs only and were then treated with hPTH, Group 3 mice received MNCs and MSCs, and were treated with hPTH.
  
  
  RESULTS
  Engraftment was achieved in all the mice. Bone marrow cellularity was approximately 20% in Group 1, but 70-80% in the hPTH treated groups. Transplantation of MNCs together with MSCs had no additional effect on bone marrow cellularity. However, the proportion of human CD13 and CD33 myeloid progenitor cells was higher in Group 3, while the proportion of human CD34 did not differ significantly between the three groups. The proportion of CXCR4 cells in Group 3 was larger than in Groups 1 and 2 but without statistical significance.
  
  
  CONCLUSIONS
  We have demonstrated a positive effect of hPTH on stem cell proliferation and a possible synergistic effect of MSCs and hPTH on the proportion of human hematopoietic progenitor cells, in a xenotransplantation model. Clinical trials of the use of hPTH after stem cell transplantation should be considered.
authors.affiliation: Department of Pediatrics, Chungnam National University Hospital, Daejeon, Korea.
authors.forename: Yeon-Jung
authors.initials: YJ
authors.lastname: Lim
authors.forename: Kyoujung
authors.initials: K
authors.lastname: Hwang
authors.forename: Miyeon
authors.initials: M
authors.lastname: Kim
authors.forename: Youl-Hee
authors.initials: YH
authors.lastname: Cho
authors.forename: Jong-Hwa
authors.initials: JH
authors.lastname: Lee
authors.forename: Young-Ho
authors.initials: YH
authors.lastname: Leelee
authors.forename: Jong-Jin
authors.initials: JJ
authors.lastname: Seo
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D049109:Cell Proliferation; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D010281:Parathyroid Hormone / Q000627:therapeutic use*; D013234:Stem Cells / Q000166:cytology; D014183:Transplantation, Heterologous
chemical_list: D010281:Parathyroid Hormone