title: Micro-structured polymer scaffolds fabricated by direct laser writing for tissue engineering.
abstract: This work presents the latest results on direct laser writing of polymeric materials for tissue engineering applications. A femtosecond Yb:KGW laser (300 fs, 200 kHz, 515 nm) was used as a light source for non-linear lithography. Fabrication was implemented in various photosensitive polymeric materials, such as: hybrid organic-inorganic sol-gel based on silicon-zirconium oxides, commercial ORMOCER® class photoresins. These materials were structured via multi-photon polymerization technique with submicron resolution. Porous three-dimensional scaffolds for artificial tissue engineering were fabricated with constructed system and were up to several millimeters in overall size with 10 to 100 μm internal pores. Biocompatibility of the used materials was tested in primary rabbit muscle-derived stem cell culture in vitro and using laboratory rats in vivo. This interdisciplinary study suggests that proposed technique and materials are suitable for tissue engineering applications.
authors.affiliation: Vilnius University, Faculty of Physics, Department of Quantum Electronics, Laser Research Center, Sauletekio Avenue 10, LT-10223 Vilnius, Lithuania.
authors.forename: Paulius
authors.initials: P
authors.lastname: Danilevicius
authors.forename: Sima
authors.initials: S
authors.lastname: Rekstyte
authors.forename: Evaldas
authors.initials: E
authors.lastname: Balciunas
authors.forename: Antanas
authors.initials: A
authors.lastname: Kraniauskas
authors.forename: Rasa
authors.initials: R
authors.lastname: Jarasiene
authors.forename: Raimondas
authors.initials: R
authors.lastname: Sirmenis
authors.forename: Daiva
authors.initials: D
authors.lastname: Baltriukiene
authors.forename: Virginija
authors.initials: V
authors.lastname: Bukelskiene
authors.forename: Roaldas
authors.initials: R
authors.lastname: Gadonas
authors.forename: Mangirdas
authors.initials: M
authors.lastname: Malinauskas
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000528:radiation effects*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007834:Lasers; D013499:Surface Properties / Q000528:radiation effects; D023822:Tissue Engineering / Q000295:instrumentation*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials