title: Variation and their relationship of NAM-G1 gene and grain protein content in Triticum timopheevii Zhuk.
abstract: NAM is an important domestication gene and valuable to enhance grain protein contents (GPCs) of modern wheat cultivars. In the present study, 12 NAM-G1 genes in Triticum timopheevii Zhuk. (AAGG, 2n=4x=28) were cloned. These genes had the same length of 1546 bp including two introns and three exons, and encoded a polypeptide of 407 amino acid residues which contained a N-terminal NAC domain with five sub-domains, and a C-terminal transcriptional activation region (TAR). They were highly similar to the previously published functional NAM-B1 gene DQ871219 from T. turgidum ssp. dicoccoides Körn. (AABB, 2n=4x=28) in both the nucleotide and protein sequences, with a very high identity of 99.5%. The differences among the 12 NAM-G1 genes resulted from 17 SNPs including 14 transitions and 3 transversions. They had outstandingly different expression levels in qRT-PCR. And, their relative expression quantities were significantly positively correlated with GPC of the accessions. In addition, the difference in amino acid sequences of the NAM-G1 genes may also affect the GPC variation.
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, PR China.
authors.forename: Xi-Gui
authors.initials: XG
authors.lastname: Hu
authors.forename: Bi-Hua
authors.initials: BH
authors.lastname: Wu
authors.forename: Deng-Cai
authors.initials: DC
authors.lastname: Liu
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Wei
authors.forename: Shi-Bin
authors.initials: SB
authors.lastname: Gao
authors.forename: You-Liang
authors.initials: YL
authors.lastname: Zheng
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000302:isolation & purification; D017343:Genes, Plant; D010940:Plant Proteins / Q000032:analysis*; D012313:RNA / Q000302:isolation & purification; D012639:Seeds / Q000737:chemistry*; D013045:Species Specificity; D014157:Transcription Factors / Q000032:analysis*; D014908:Triticum / Q000737:chemistry* / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D014157:Transcription Factors; D012313:RNA