title: Single-step rapid assembly of DNA origami nanostructures for addressable nanoscale bioreactors.
abstract: Self-assembled DNA origami nanostructures have shown great promise for bottom-up construction of complex objects with nanoscale addressability. Here we show that DNA origami-based 1D nanoribbons and nanotubes are one-pot assembled with controllable sizes and nanoscale addressability with high speed (within only 10-20 min), exhibiting extraordinarily high cooperativity that is often observed in assembly of natural molecular machines in cells (e.g. ribosome). By exploiting the high specificity of DNA-based self-assembly, we can precisely anchor proteins on these DNA origami nanostructures with sub-10 nm resolution and at the single-molecule level. We attach a pair of enzymes (horseradish peroxidase and glucose oxidase) at the inner side of DNA nanotubes and observe high coupling efficiency of enzyme cascade within this confined nanospace. Hence, DNA nanostructures with such unprecedented properties shed new light on the design of nanoscale bioreactors and nanomedicine and provide an artificial system for studying enzyme activities and cascade in highly organized and crowded cell-mimicking environments.
authors.affiliation: Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Fu
authors.forename: Dongdong
authors.initials: D
authors.lastname: Zeng
authors.forename: Jie
authors.initials: J
authors.lastname: Chao
authors.forename: Yanqiu
authors.initials: Y
authors.lastname: Jin
authors.forename: Zhao
authors.initials: Z
authors.lastname: Zhang
authors.forename: Huajie
authors.initials: H
authors.lastname: Liu
authors.forename: Di
authors.initials: D
authors.lastname: Li
authors.forename: Hongwei
authors.initials: H
authors.lastname: Ma
authors.forename: Qing
authors.initials: Q
authors.lastname: Huang
authors.forename: Kurt V
authors.initials: KV
authors.lastname: Gothelf
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
mesh_terms: D019149:Bioreactors; D004247:DNA / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology
chemical_list: D004247:DNA