title: Effects of microbial elicitor on production of hypocrellin by Shiraia bambusicola.
abstract: Some endophyte isolates were isolated in a bamboo pole sample parasitized the fungus Shiraia bambusicola from Zhejiang Province. After screening through hypocrellin bacteriostatic effect and fermentation test, we got the isolate TX4 of bacterial elicitor and GZUIFR-TT1 of fungal elicitor which had certain effect to promote S. bambusicola to produce hypocrellin. The Plackett-Burman design was introduced to evaluate the effects of nine factors based on single-factor test. Yeast extract, glucose, and isolate GZUIFR-TT1 elicitor were found to be the critical activity factors for increasing the total hypocrellin production. So we identified the isolate GZUIFR-TT1 as Trametes sp. Through response surface methodology, we obtained the optimum production conditions as follows: yeast extract, 2.99 g/L; glucose, 32.45 g/L; and Trametes sp. elicitor, 81.40 μg/mL. Under the above conditions, the experimental value of hypocrellin production was 102.60 mg/L, compared with the control it increased about 7.90 times.
authors.affiliation: Institute of Fungus Resources, Guizhou University, Guiyang, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Du
authors.forename: Zongqi
authors.initials: Z
authors.lastname: Liang
authors.forename: Xiao
authors.initials: X
authors.lastname: Zou
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Han
authors.forename: Jiandong
authors.initials: J
authors.lastname: Liang
authors.forename: Jianping
authors.initials: J
authors.lastname: Yu
authors.forename: Wanhao
authors.initials: W
authors.lastname: Chen
authors.forename: Yurong
authors.initials: Y
authors.lastname: Wang
authors.forename: Chunlong
authors.initials: C
authors.lastname: Sun
mesh_terms: D000890:Anti-Infective Agents / Q000378:metabolism*; D001203:Ascomycota / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods; D003470:Culture Media / Q000737:chemistry; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005285:Fermentation; D008969:Molecular Sequence Data; D010569:Perylene / Q000031:analogs & derivatives* / Q000378:metabolism; D019800:Phenol; D011809:Quinones / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D055454:Trametes / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification
chemical_list: D000890:Anti-Infective Agents; D003470:Culture Media; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D011809:Quinones; D019800:Phenol; D010569:Perylene; C048388:hypocrellin A