title: Effects of dietary history on energy metabolism and physiological parameters in C57BL/6J mice.
abstract: Understanding body weight regulation is essential to fight obesity. Mouse studies, using different types of diets, showed conflicting results in terms of body weight persistence after changing from an ad libitum high-fat diet to an ad libitum low-fat diet. In this study, we questioned specifically whether the energy content of the diet has a lasting effect on energy balance and body weight, using multiple switches and two purified diets with a different fat-to-sugar ratio, but otherwise identical ingredients. Young-adult obesity-prone male C57BL/6J mice were fed single or double switches of semi-purified diets with either 10 energy % (en%) fat (LF) or 40en% fat (HF), with starch replaced by fat, while protein content remained equal. After none, one or two dietary changes, energy metabolism was assessed at 5, 14 and 19 weeks. We observed no systematic continuous compensation in diet and energy intake when returning to LF after HF consumption. Body weight, white adipose tissue mass and histology, serum metabolic parameters, energy expenditure and substrate usage all significantly reflected the current diet intake, independent of dietary changes. This contrasts with studies that used diets with different ingredients and showed persistent effects of dietary history on body weight, suggesting diet-dependent metabolic set points. We conclude that body weight and metabolic parameters 'settle', based on current energetic input and output. This study also highlights the importance of considering the choice of diet in physiological and metabolic intervention studies.
authors.affiliation: Human and Animal Physiology, Wageningen University, De Elst 1, 6708 WD Wageningen, PO Box 338, 6700AH Wageningen, The Netherlands.
authors.forename: Femke P M
authors.initials: FP
authors.lastname: Hoevenaars
authors.forename: Jaap
authors.initials: J
authors.lastname: Keijer
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Swarts
authors.forename: Sophie
authors.initials: S
authors.lastname: Snaas-Alders
authors.forename: Melissa
authors.initials: M
authors.lastname: Bekkenkamp-Grovenstein
authors.forename: Evert M
authors.initials: EM
authors.lastname: van Schothorst
mesh_terms: D052436:Adipose Tissue, White / Q000473:pathology; D000818:Animals; D001835:Body Weight / Q000187:drug effects*; D002151:Calorimetry; D018752:Diet, Fat-Restricted; D059305:Diet, High-Fat; D004041:Dietary Fats / Q000494:pharmacology*; D002149:Energy Intake / Q000502:physiology*; D004734:Energy Metabolism / Q000502:physiology*; D020738:Leptin / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000378:metabolism / Q000473:pathology
chemical_list: D004041:Dietary Fats; D020738:Leptin