title: The ultrastructural difference between CD133-positive U251 glioma stem cells and normal U251 glioma cells.
abstract: Glioma stem cells (GSC) have higher tumorigenic potential and stronger chemoresistance and radioresistance than normal glioma cells. The mechanisms behind these phenomena have remained elusive. The authors have isolated CD133-positive U251 GSCs from U251 glioma cells and detected the expression of stem cell markers (CD133 and nestin) of U251 GSCs by immunofluorescence staining. Then the ultrastructures of U251 GSCs and normal U251 glioma cells were observed by transmission electron microscopy and the ultrastructural differences between them were compared. Increased cell nucleus atypia, rougher endoplasmic reticulum, and more microvilli were observed in CD133-positive U251 GSCs than in normal U251 glioma cells. In summary, these ultrastructural differences support the hypothesis that GSCs have stronger tumorigenic ability and resistance to chemotherapy and radiotherapy.
authors.affiliation: Department of Radiation and Medical Oncology, Zhongnan Hospital, Wuhan University, Wuchang District, Wuhan, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.forename: You
authors.initials: Y
authors.lastname: Wang
authors.forename: Chunxu
authors.initials: C
authors.lastname: Yang
authors.forename: Wen
authors.initials: W
authors.lastname: Ouyang
authors.forename: Fuxiang
authors.initials: F
authors.lastname: Zhou
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Zhou
authors.forename: Conghua
authors.initials: C
authors.lastname: Xie
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000378:metabolism*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D005910:Glioma / Q000276:immunology / Q000648:ultrastructure*; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D008871:Microvilli; D014411:Neoplastic Stem Cells / Q000276:immunology / Q000648:ultrastructure*; D010455:Peptides / Q000378:metabolism*; D010641:Phenotype
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides