title: [Study on content of two sesquiterpenes in Alpinia oxyphylla].
abstract: OBJECTIVE
  To develop an HPLC method for determining oxyphyllenodiol A (1) and teuhetenone A (2) contained in Alpinia oxyphylla and to compare the contents of the two components contained in medicinal materials and prepared herbal medicines in pieces sold in the market and different fractions.
  
  
  METHODS
  HPLC and Waters sunfire C18 column (4. 6 mm x 250 mm, 5 microm) were adopted for gradient elute with the mobile phase of acetonitrile and water. The flow rate was 1.0 mL x min(-1) and the detection wavelength was 250 nm.
  
  
  RESULTS
  1 and 2 showed a good linear relationship within the range of 0.1296 - 0.8640 microg and 0.1635 - 1.0900 microg respectively, with the average recoveries of 99.08% and 97.80%. Their content ranges were 0.0059% - 0.0149% and 0.0080% - 0.0164% in different samples. The mean value of 1 and 2 were 0.0085% and 0.0104% in the whole fruits, and 0.0137% and 0.0157% in the seeds. They were undetected in the nutshells.
  
  
  CONCLUSIONS
  The method is so precise, accurate and highly reproducible that it can be used to determine the contents of oxyphyllenodiol A and teuhetenone A in A. oxyphylla. The contents of the two components are mainly extracted from the seeds, with certain difference among different samples. There are a higher contents and no significant difference in the salted and raw seeds.
authors.affiliation: Institute of Medical Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China.
authors.forename: Xiu-xiang
authors.initials: XX
authors.lastname: Lv
authors.forename: Lei
authors.initials: L
authors.lastname: Hou
authors.forename: Wen-hua
authors.initials: WH
authors.lastname: Huang
authors.forename: Bao-lin
authors.initials: BL
authors.lastname: Guo
authors.forename: Lian-xue
authors.initials: LX
authors.lastname: Zhang
authors.forename: Jing-guang
authors.initials: JG
authors.lastname: Yu
mesh_terms: D032429:Alpinia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000302:isolation & purification; D012717:Sesquiterpenes / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D004365:Drugs, Chinese Herbal; D012717:Sesquiterpenes