title: Analysis of losartan and carvedilol in urine and plasma samples using a dispersive liquid-liquid microextraction isocratic HPLC-UV method.
abstract: BACKGROUND
  A simple, precise and sensitive HPLC method has been developed for simultaneous determination of carvedilol and losartan in human plasma and urine samples. The analytes were extracted by a dispersive liquid-liquid microextraction method. A mobile phase of 15 mM sodium dihydrogen phosphate buffer (pH 4.0)/acetonitrile/2-propanol (70/27.5/2.5, v/v/v) was used to separate the drugs using a Waters® ODS column (250 × 4.6 mm) and detected by a UV detector at 222 nm.
  
  
  RESULTS
  The developed method is selective for studied drugs possessing a linearity range of 0.1-1.0 and 0.05-0.75 µg/ml, respectively, for losartan and carvedilol with precision <15%. The accuracy is better than 15% and the mean recovery of carvedilol and losartan was 98.9 and 100.2% for plasma and 100.7 and 100.5% for urine samples, respectively.
  
  
  CONCLUSIONS
  The developed method is applicable for therapeutic drug monitoring and PK analyses.
authors.affiliation: Cardiovascular Research Center, Tabriz University of Medical Sciences, Tabriz 51664, Iran.
authors.forename: Somaieh
authors.initials: S
authors.lastname: Soltani
authors.forename: Amir M
authors.initials: AM
authors.lastname: Ramezani
authors.forename: Naser
authors.initials: N
authors.lastname: Soltani
authors.forename: Abolghasem
authors.initials: A
authors.lastname: Jouyban
mesh_terms: D000959:Antihypertensive Agents / Q000032:analysis* / Q000302:isolation & purification / Q000493:pharmacokinetics; D002227:Carbazoles / Q000032:analysis* / Q000097:blood / Q000652:urine; D000077261:Carvedilol; D002851:Chromatography, High Pressure Liquid; D006207:Half-Life; D006801:Humans; D006863:Hydrogen-Ion Concentration; D059627:Liquid Phase Microextraction; D019808:Losartan / Q000032:analysis* / Q000097:blood / Q000652:urine; D011412:Propanolamines / Q000032:analysis* / Q000097:blood / Q000652:urine; D012492:Salts / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D016482:Urinalysis / Q000295:instrumentation / Q000379:methods*
chemical_list: D000959:Antihypertensive Agents; D002227:Carbazoles; D011412:Propanolamines; D012492:Salts; D012997:Solvents; D000077261:Carvedilol; D019808:Losartan