title: Procyanidins modulate microRNA expression in pancreatic islets.
abstract: Procyanidins modulate glucose metabolism, partly due to its effects on pancreas. Given the role of microRNAs (miRNAs) in the regulation of diabetes and the fact that flavonoids modulate miRNAs in other tissues, we hypothesized that procyanidins might target miRNAs in the pancreas. We investigated the miRNA expression profile in pancreatic islets isolated from rats treated with a daily dose of grape seed procyanidin extract (GSPE) (25 mg/kg of body weight) for 45 days. The miRWalk database identified putative target genes of these miRNAs. We found that GSPE altered significantly the expression of miR-1249, miR-483, miR-30c-1*, and miR-3544. In silico prediction studies suggested that ion transport and response to glucose are among the regulated pathways. As a conclusion, this is the first study showing that procyanidins can also exert their bioactivity on pancreatic islets by modifying the miRNA expression pattern.
authors.affiliation: Nutrigenomics Research Group, Departament de Bioquímica i Biotecnologia, Universitat Rovira i Virgili, Marcel·lí Domingo s/n, 43007 Tarragona, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Castell-Auví
authors.forename: Lídia
authors.initials: L
authors.lastname: Cedó
authors.forename: Jamileh
authors.initials: J
authors.lastname: Movassat
authors.forename: Bernard
authors.initials: B
authors.lastname: Portha
authors.forename: Fátima
authors.initials: F
authors.lastname: Sánchez-Cabo
authors.forename: Victor
authors.initials: V
authors.lastname: Pallarès
authors.forename: Mayte
authors.initials: M
authors.lastname: Blay
authors.forename: Montserrat
authors.initials: M
authors.lastname: Pinent
authors.forename: Anna
authors.initials: A
authors.lastname: Ardévol
mesh_terms: D000818:Animals; D017209:Apoptosis; D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation; D019295:Computational Biology; D019587:Dietary Supplements; D015536:Down-Regulation; D005103:Expert Systems; D005260:Female; D020869:Gene Expression Profiling; D056604:Grape Seed Extract / Q000378:metabolism*; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism*; D035683:MicroRNAs / Q000378:metabolism*; D044945:Proanthocyanidins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; D056604:Grape Seed Extract; C511402:Grape Seed Proanthocyanidins; D035683:MicroRNAs; D044945:Proanthocyanidins