title: DOCA sensitive pendrin expression in kidney, heart, lung and thyroid tissues.
abstract: OBJECTIVE
  Pendrin (SLC26A4), a transporter accomplishing anion exchange, is expressed in inner ear, thyroid gland, kidneys, lung, liver and heart. Loss or reduction of function mutations of SLC26A4 underlie Pendred syndrome, a disorder invariably leading to hearing loss with enlarged vestibular aqueducts and in some patients to hypothyroidism and goiter. Renal pendrin expression is up-regulated by mineralocorticoids such as aldosterone or deoxycorticosterone (DOCA). Little is known about the impact of mineralocorticoids on pendrin expression in extrarenal tissues.
  
  
  METHODS
  The present study utilized RT-qPCR and Western blotting to quantify the transcript levels and protein abundance of Slc26a4 in murine kidney, thyroid, heart and lung prior to and following subcutaneous administration of 100 mg/kg DOCA.
  
  
  RESULTS
  Slc26a4 transcript levels as compared to Gapdh transcript levels were significantly increased by DOCA treatment in kidney, heart, lung and thyroid. Accordingly pendrin protein expression was again significantly increased by DOCA treatment in kidney, heart, lung and thyroid.
  
  
  CONCLUSIONS
  The observations reveal mineralocorticoid sensitivity of pendrin expression in kidney, heart, thyroid and lung.
authors.affiliation: Department of Physiology, University of Tuebingen, Tuebingen, Germany.
authors.forename: Lisann
authors.initials: L
authors.lastname: Pelzl
authors.forename: Tatsiana
authors.initials: T
authors.lastname: Pakladok
authors.forename: Ganesh
authors.initials: G
authors.lastname: Pathare
authors.forename: Hajar
authors.initials: H
authors.lastname: Fakhri
authors.forename: Diana
authors.initials: D
authors.lastname: Michael
authors.forename: Carsten A
authors.initials: CA
authors.lastname: Wagner
authors.forename: Markus
authors.initials: M
authors.lastname: Paulmichl
authors.forename: Florian
authors.initials: F
authors.lastname: Lang
mesh_terms: D000818:Animals; D027321:Anion Transport Proteins / Q000235:genetics / Q000378:metabolism*; D003900:Desoxycorticosterone / Q000502:physiology*; D005260:Female; D007668:Kidney / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D008297:Male; D051379:Mice; D008901:Mineralocorticoids / Q000502:physiology; D009206:Myocardium / Q000378:metabolism*; D009928:Organ Specificity; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000076988:Sulfate Transporters; D013961:Thyroid Gland / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D027321:Anion Transport Proteins; D008901:Mineralocorticoids; C494849:Slc26a4 protein, mouse; D000076988:Sulfate Transporters; D003900:Desoxycorticosterone