title: Association of p21 SNPs and risk of cervical cancer among Chinese women.
abstract: BACKGROUND
  The p21 codon 31 single nucleotide polymorphism (SNP), rs1801270, has been linked to cervical cancer but with controversial results. The aims of this study were to investigate the role of p21 SNP-rs1801270 and other untested p21 SNPs in the risk of cervical cancer in a Chinese population.
  
  
  METHODS
  We genotyped five p21 SNPs (rs762623, rs2395655, rs1801270, rs3176352, and rs1059234) using peripheral blood DNA from 393 cervical cancer patients and 434 controls.
  
  
  RESULTS
  The frequency of the rs1801270 A allele in patients (0.421) was significantly lower than that in controls (0.494, p = 0.003). The frequency of the rs3176352 C allele in cases (0.319) was significantly lower than that in controls (0.417, p < 0.001).The allele frequency of other three p21 SNPs showed not statistically significantly different between patients and controls. The rs1801270 AA genotype was associated with a decreased risk for the development of cervical cancer (OR = 0.583, 95%CI: 0.399 - 0.853, P = 0.005). We observed that the three p21 SNPs (rs1801270, rs3176352, and rs1059234) was in linkage disequilibrium (LD) and thus haplotype analysis was performed. The AGT haplotype (which includes the rs1801270A allele) was the most frequent haplotype among all subjects, and both homozygosity and heterozygosity for the AGT haplotype provided a protective effect from development of cervical cancer.
  
  
  CONCLUSIONS
  We show an association between the p21 SNP rs1801270A allele and a decreased risk for cervical cancer in a population of Chinese women. The AGT haplotype formed by three p21 SNPs in LD (rs1801270, rs3176352 and rs1059234) also provided a protective effect in development of cervical cancer in this population.
authors.affiliation: Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Shizhuo
authors.initials: S
authors.lastname: Wang
authors.forename: Qiao
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yanming
authors.initials: Y
authors.lastname: Lu
authors.forename: Heng
authors.initials: H
authors.lastname: Wei
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Shulan
authors.initials: S
authors.lastname: Zhang
authors.forename: Duo
authors.initials: D
authors.lastname: Yin
authors.forename: Yangling
authors.initials: Y
authors.lastname: Ou
mesh_terms: D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D003062:Codon / Q000235:genetics; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D015810:Linkage Disequilibrium / Q000235:genetics; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors; D002583:Uterine Cervical Neoplasms / Q000209:etiology* / Q000235:genetics*
chemical_list: D003062:Codon; D050759:Cyclin-Dependent Kinase Inhibitor p21