title: [Immune efficacy of rabies virus glycoprotein expressed by baculovirus vector].
abstract: To construct a recombinant baculovirus expressing glycoprotein (GP) of RV SRV9 strain and test the immunological efficacy in mice, open reading frame of rabies virus GP gene of SRV9 strain was cloned into the shuttle vector Bacmid to construct the recombinant shuttle plasmid Bacmid-G and transfection was performed into S f9 cells with the recombinant shuttle plasmid. CPE appeared in cell cultures was identified by electronmicroscopy. Western-blot, IFA and immunity tests in mice were performed to identify the immunoreactivity and immunogenicity of the expression products. Our results showed a recombinant baculovirus expressing GP protein of rabies virus SRV9 was obtained. The expression products possessed a favorable immunogenicity and fall immunized mice could develop 100% protective level of anti-rabies neutralizing antibody. In conclusion, The SRV9 glycoprotein expressed by the recombinant baculovirus in this study had good immunogenicity and could induce anti-rabies neutralizing antibody, which laid the foundation of further development of rabies subunit vaccine.
authors.affiliation: Military Veterinary Research Institute, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Control and Prevention, Changchun 130122, China. chenqi19880804@163.com
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.forename: Shou-Feng
authors.initials: SF
authors.lastname: Zhang
authors.forename: Ye
authors.initials: Y
authors.lastname: Liu
authors.forename: Yun-Hong
authors.initials: YH
authors.lastname: Fu
authors.forename: Cheng-Long
authors.initials: CL
authors.lastname: Sun
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Ting
authors.initials: T
authors.lastname: Gong
authors.forename: Fei-Fei
authors.initials: FF
authors.lastname: Song
authors.forename: Rong-Liang
authors.initials: RL
authors.lastname: Hu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006023:Glycoproteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D006801:Humans; D051379:Mice; D011818:Rabies / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D011819:Rabies Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D011820:Rabies virus / Q000235:genetics / Q000276:immunology*; D014764:Viral Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D006023:Glycoproteins; D011819:Rabies Vaccines; D014764:Viral Proteins