title: Microbiological profile and antimicrobial susceptibility pattern of infected root canals associated with periapical abscesses.
abstract: The aim of this investigation was to identify microorganisms from root canals with periapical abscesses and assess the susceptibility of specific anaerobic bacteria to selected antimicrobials and their β-lactamase production. Sixty root canals were microbiologically investigated. The susceptibility of Anaerococcus prevotii, Fusobacterium necrophorum, F. nucleatum, Parvimonas micra, and Prevotella intermedia/nigrescens to antimicrobials was evaluated with the Etest, whereas β-lactamase production was assessed with nitrocefin. A total of 287 different bacterial strains were recovered, including 201 strict anaerobes. The most frequently strict isolated anaerobes were A. prevotii, P. micra, and F. necrophorum. The selected bacteria were susceptible to all the tested antibiotics, except A. prevotii and Fusobacterium species to azithromycin and erythromycin, as well as A. prevotii and F. necrophorum to metronidazole. None of the microorganisms produced β-lactamase. Gram-positive anaerobic bacteria predominated in the root canals with periapical abscesses. All microorganisms tested were susceptible to benzylpenicillin, amoxicillin, amoxicillin + clavulanate, cefaclor, and clindamycin, producing no β-lactamase.
authors.affiliation: Department of Conservative Dentistry, Endodontics Division, Pelotas School of Dentistry, Federal University of Pelotas, Pelotas, RS, Brazil.
authors.forename: E L R
authors.initials: EL
authors.lastname: Sousa
authors.forename: B P F A
authors.initials: BP
authors.lastname: Gomes
authors.forename: R C
authors.initials: RC
authors.lastname: Jacinto
authors.forename: A A
authors.initials: AA
authors.lastname: Zaia
authors.forename: C C R
authors.initials: CC
authors.lastname: Ferraz
mesh_terms: D000293:Adolescent; D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001421:Bacteria, Anaerobic / Q000145:classification* / Q000187:drug effects* / Q000302:isolation & purification; D044822:Biodiversity; D002648:Child; D003786:Dental Pulp Cavity / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D010482:Periapical Abscess / Q000382:microbiology*; D055815:Young Adult; D001618:beta-Lactamases / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases