title: [Preparation and identification of monoclonal antibody against human spermine oxidase].
abstract: OBJECTIVE
  To prepare mouse anti-human spermine oxidase  (anti-hSMO) monoclonal antibody (mAb) and testify its application  in the biological techniques including Western blotting  and immunohistochemistry.
  
  
  METHODS
  Plasmid pET-15b/SMO was first transferred into BL21 (DE3),  and then SMO recombinant protein with 6×His tag was induced to express by IPTG and purified by Ni-NTA resin. The purified recombinant SMO was used to immunize BALB/c mouse. The spleen cells from the immunized mouse were harvested and hybridized with Sp2/0 myeloma cells to obtain a hybridoma cell line that could efficiently synthesize and secret anti-SMO mAb. The titer and antigen specificity of this antibody were identified by ELISA,  Western blotting  and immunohistochemistry.
  
  
  RESULTS
  We successfully obtained the hybridoma cell line which could stably secret anti-SMO mAb. The mAb was of a high titer and antigen specificity and could be used in ELISA,  Western blotting,  and immunohistochemistry  for SMO.
  
  
  CONCLUSIONS
  The mouse anti-hSMO mAB with a high antigen specificity has been prepared successfully and used for a variety of bio-analytical techniques.
authors.affiliation: Institute of Molecular Biology,  Medical College,  Three Gorges University,  Yichang 443002,  China.
authors.forename: Chun-hong
authors.initials: CH
authors.lastname: Qinli
authors.forename: Jian-lin
authors.initials: JL
authors.lastname: Yang
authors.forename: Yu
authors.initials: Y
authors.lastname: Han
authors.forename: Fu-qiang
authors.initials: FQ
authors.lastname: Cai
authors.forename: Yan-lin
authors.initials: YL
authors.lastname: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000918:Antibody Specificity; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007114:Immunization; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D000097667:Polyamine Oxidase
chemical_list: D000911:Antibodies, Monoclonal; D000587:Oxidoreductases Acting on CH-NH Group Donors; D000097667:Polyamine Oxidase