title: Circulating miR-30a, miR-195 and let-7b associated with acute myocardial infarction.
abstract: BACKGROUND
  MicroRNAs (miRNAs) play key roles in diverse biological and pathological processes, including the regulation of proliferation, apoptosis, angiogenesis and cellular differentiation. Recently, circulating miRNAs have been reported as potential biomarkers for various pathologic conditions. This study investigated miR-30a, miR-195 and let-7b as potential of biomarker for acute myocardial infarction (AMI).
  
  
  RESULTS
  Plasma samples from 18 patients with AMI and 30 healthy adults were collected. Total RNA was extracted from plasma with TRIzol LS Reagent. MiRNA levels and plasma cardiac troponin I (cTnI) concentrations were measured by quantitative real-time PCR and ELISA assay, respectively. Results showed that circulating miR-30a in AMI patients was highly expressed at 4 h, 8 h and 12 h after onset of AMI, and miR-195 was highly expressed at 8 h and 12 h. However, let-7b was lower in AMI patients than in controls throughout the whole time points. Interestingly, in these patients, circulating miR-30a, miR-195 and let-7b all reached their expression peak at 8 h. By the receiver operating characteristic (ROC) curve analyses, these plasma miRNAs were of significant diagnostic value for AMI. The combined ROC analysis revealed the an AUC value of 0.93 with 94% sensitivity and 90% specificity at 8 h after onset, and an AUC value of 0.92 with 90% sensitivity and 90% specificity at 12 h after onset, in discriminating the AMI patients from healthy controls.
  
  
  CONCLUSIONS
  Our results imply that the plasma concentration of miR-30a, miR-195 and let-7b can be potential indicators for AMI.
authors.affiliation: Department of Internal Medicine and the Institute of Hypertension, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, PR China.
authors.forename: Guangwen
authors.initials: G
authors.lastname: Long
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Quanlu
authors.initials: Q
authors.lastname: Duan
authors.forename: Shenglan
authors.initials: S
authors.lastname: Yang
authors.forename: Fuqiong
authors.initials: F
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Gong
authors.forename: Xu
authors.initials: X
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Dao Wen
authors.initials: DW
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood / Q000175:diagnosis*; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; C552722:MIRN195 microRNA, human; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; C487931:mirnlet7 microRNA, human