title: A biomimetic mechanism for antibody immobilization on lipid nanofibers for cell capture.
abstract: The immobilization of membrane-bound molecules on organic-inorganic cholesteryl-succinyl silane (CSS) nanofibers is investigated. Fluorescent microscopy and a cell capture assay confirm the stable and functional immobilization of membrane-bound antibodies and imaging agents on the electrospun CSS nanofibers. An insert-and-tighten mechanism is proposed for the observed hydration-induced reduction in lipid nanofiber diameter, the immobilization of membrane-bound molecules, and the improved efficiency of cell capture by the functionalized CSS nanofibers over their film counterparts. The ability to stably and functionally immobilize membrane-bound molecules on the CSS nanofibers presents a promising method to functionalize lipid-based nanomaterials.
authors.affiliation: Department of Aerospace and Mechanical Engineering, University of Arizona, Tucson, Arizona 85721, USA.
authors.forename: Zhengbao
authors.initials: Z
authors.lastname: Zha
authors.forename: Linan
authors.initials: L
authors.lastname: Jiang
authors.forename: Zhifei
authors.initials: Z
authors.lastname: Dai
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Wu