title: Green ultra-fast high-performance liquid chromatographic method using a short narrow-bore column packed with fully porous sub-2 μm particles for the simultaneous determination of selected pharmaceuticals as surface water and wastewater pollutants.
abstract: Fast separations are very desirable in laboratories that analyze large numbers of samples per day or those needing short turn-around times. Traditional HPLC methods using conventional stationary phases and standard column dimensions require significant amounts of organic solvents and generate large volumes of waste. With growing awareness about the environment, the development of green technologies has been receiving increasing attention. In this work, a very fast green analytical method based on LC-UV using a short narrow bore column packed with fully porous sub-2 μm particles has been developed for simultaneous determination of nine pharmaceuticals in wastewater and surface water. The chromatographic separation was optimized in order to achieve short analysis time and good resolution for all analytes in a single run. All analytes could be separated in 1 min with good resolution. Sample preparation was executed by solid phase extraction using Oasis HLB cartridges. The method developed was validated based on parameters such as linearity, precision, accuracy, detection, and quantification limits. The recovery ranged from 70.9 to 92.5% with SDs not higher than 5.4%, except for acetaminophen and sulphanilamide. LODs ranged from 0.6-2.5 μg/L, while the LOQs were in the range 2-8 μg/L.
authors.affiliation: Department of Chemistry, University of Waterloo, Waterloo, Ontario, Canada.
authors.forename: Heba
authors.initials: H
authors.lastname: Shaaban
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Górecki
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D055772:Green Chemistry Technology; D004364:Pharmaceutical Preparations / Q000737:chemistry* / Q000302:isolation & purification; D016062:Porosity; D012117:Resins, Synthetic / Q000737:chemistry; D052616:Solid Phase Extraction; D062065:Wastewater / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D004364:Pharmaceutical Preparations; D012117:Resins, Synthetic; D062065:Wastewater; D014874:Water Pollutants, Chemical