title: NOX2 deficiency attenuates markers of adiposopathy and brain injury induced by high-fat diet.
abstract: The consumption of high-fat/calorie diets in modern societies is likely a major contributor to the obesity epidemic, which can increase the prevalence of cancer, cardiovascular disease, and neurological impairment. Obesity may precipitate decline via inflammatory and oxidative signaling, and one factor linking inflammation to oxidative stress is the proinflammatory, pro-oxidant enzyme NADPH oxidase. To reveal the role of NADPH oxidase in the metabolic and neurological consequences of obesity, the effects of high-fat diet were compared in wild-type C57Bl/6 (WT) mice and in mice deficient in the NAPDH oxidase subunit NOX2 (NOX2KO). While diet-induced weight gains in WT and NOX2KO mice were similar, NOX2KO mice had smaller visceral adipose deposits, attenuated visceral adipocyte hypertrophy, and diminished visceral adipose macrophage infiltration. Moreover, the detrimental effects of HFD on markers of adipocyte function and injury were attenuated in NOX2KO mice; NOX2KO mice had improved glucose regulation, and evaluation of NOX2 expression identified macrophages as the primary population of NOX2-positive cells in visceral adipose. Finally, brain injury was assessed using markers of cerebrovascular integrity, synaptic density, and reactive gliosis, and data show that high-fat diet disrupted marker expression in WT but not NOX2KO mice. Collectively, these data indicate that NOX2 is a significant contributor to the pathogenic effects of high-fat diet and reinforce a key role for visceral adipose inflammation in metabolic and neurological decline. Development of NOX-based therapies could accordingly preserve metabolic and neurological function in the context of metabolic syndrome.
authors.affiliation: Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA 70808, USA.
authors.forename: Jennifer K
authors.initials: JK
authors.lastname: Pepping
authors.forename: Linnea R
authors.initials: LR
authors.lastname: Freeman
authors.forename: Sunita
authors.initials: S
authors.lastname: Gupta
authors.forename: Jeffrey N
authors.initials: JN
authors.lastname: Keller
authors.forename: Annadora J
authors.initials: AJ
authors.lastname: Bruce-Keller
mesh_terms: D050154:Adiposity; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D059305:Diet, High-Fat / Q000009:adverse effects*; D006984:Hypertrophy; D050152:Intra-Abdominal Fat / Q000276:immunology / Q000473:pathology; D008264:Macrophages / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D024821:Metabolic Syndrome / Q000209:etiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D009765:Obesity / Q000276:immunology / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D018384:Oxidative Stress; D021122:Protein Subunits / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D015430:Weight Gain
chemical_list: D015415:Biomarkers; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D021122:Protein Subunits; C488842:Cybb protein, mouse; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases