title: [Migration of PKH26-labeled mesenchymal stem cells in rats with Alzheimer's disease].
abstract: To investigate the migration of fluorescent dye PKH26-labeled BM-MSC in the Alzheimer's model rats.
  
  
  
  Normal human bone marrow extracted for isolation of BM-MSC was cultured in vitro. The 5th passaged BM-MSC was labeled with PKH26, and observed under a fluorescence microscope for PKH26 labeling efficiency, and using flow cytometry BM-MSC surface markers was checked. The PKH26 labeled BM-MSC injected into the tail vein of the normal control group and AD animal model group, 14 days after finding the PKH26-labeled BM-MSC cells in the rat hippocampus using fluorescence microscopy. Using the Morris water maze experiment comparison of AD model and BM-MSC transplantation group of spatial learning and memory ability.
  
  
  
  TFlow cytometry showed BM-MSC surface markers CD73 and CD105 were positive. In vitro, PKH26-labeled rate of BM-MSC was 100 %. The Morris water maze experiment comparison of BM-MSC transplantation group and AD group of animals, BM-MSC transplantation group at 13, 14 days of spatial learning and memory ability than AD animal group had significantly improved. 14 days after BM-MSCs in rat hippocampus could be found which were PKH26-positive, consistent with DAPI staining. PKH26-positive cells in animal models of AD were significantly more than those in the normal control group.
  
  
  
  BM-MSC in AD rats not only migrates through the blood-brain barrier, but also mainly survives in the hippocampus of AD rats, and it can improve AD rat model of learning disabilities.
authors.affiliation: Psychology Department, Zhejiang University, Hangzhou, China.
authors.forename: Wen-yu
authors.initials: WY
authors.lastname: Li
authors.forename: Ri-long
authors.initials: RL
authors.lastname: Jin
authors.forename: Xing-yue
authors.initials: XY
authors.lastname: Hu
mesh_terms: D000544:Alzheimer Disease / Q000473:pathology*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002465:Cell Movement; D002478:Cells, Cultured; D004195:Disease Models, Animal; D006801:Humans; D007275:Injections, Intravenous; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009930:Organic Chemicals; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D009930:Organic Chemicals; C070080:PKH 26