title: Effect of NF-κB inhibitor PDTC on a herpetic stromal keratitis mouse model.
abstract: To investigate the effect of NF-κB inhibitor PDTC on inflammation response in mice with herpetic stromal keratitis (HSK).
  
  
  
  A total of 120 female BALB/c mice, aged 4~6 weeks, were treated by scuffing the epithelium of the right cornea with the tip of a 30G syringe needle, and approximately 1 ×106PFU of HSV-1virus was seeded onto the corneal surface. The eye was then exposed to 170mJ/cm2 of UV light 7 weeks later, in order to induce the relapse of HSK. PDTC eye drops were adjusted to various concentrations (0.1, 1.0, and 10 mg/ml) with PBS; PBS only was used on control animals. All animals received PDTC or PBS eye drops 6 times/day, 1 drop/time, for 7 consecutive  days. Mice were sacrificed at 8h, and 1, 3, and 7 d after administration. Corneal tissues were prepared for the detection of IL-1β, IL-4, IL-6, and IL-12 by ELISA and histological study.
  
  
  
  Neutrophilic leukocytes and lymphocytes were found in the corneas of HSK mice. ELISA results showed fluctuating expressions of IL-1β, IL-4, IL-6, and IL-12. The IL-1β and IL-4 expressions were significantly lower in the high dose group than in the control group (P<0.05), while IL-6 and IL-12 expressions were significantly higher in the high dose group than in the control group (P<0.05).
  
  
  
  A high concentration of PDTC can suppress the expression of certain inflammatory factors in HSK mice while improving the expression of others.
authors.affiliation: Shantou University, The Chinese University of Hong Kong Joint Shantou International Eye Center, Shantou 505041, China
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Geng
authors.affiliation: Shantou University, The Chinese University of Hong Kong Joint Shantou International Eye Center, Shantou 505041, China
authors.forename: Yunliang
authors.initials: Y
authors.lastname: Shao
authors.affiliation: Guangdong Lab Animal Monitoring Institute, Guangzhou 510663,China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Shantou University, The Chinese University of Hong Kong Joint Shantou International Eye Center, Shantou 505041, China
authors.forename: Zeyi
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D003315:Cornea / Q000187:drug effects / Q000378:metabolism; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D018259:Herpesvirus 1, Human; D018664:Interleukin-12 / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D016849:Keratitis, Herpetic / Q000188:drug therapy* / Q000378:metabolism / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D009504:Neutrophils / Q000166:cytology; D011392:Proline / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D013859:Thiocarbamates / Q000008:administration & dosage / Q000627:therapeutic use*; D014466:Ultraviolet Rays
chemical_list: D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D015850:Interleukin-6; D016328:NF-kappa B; D013859:Thiocarbamates; C066229:prolinedithiocarbamate; D018664:Interleukin-12; D015847:Interleukin-4; D011392:Proline