title: Mosquito transmission of the rodent malaria parasite Plasmodium chabaudi.
abstract: BACKGROUND
  Serial blood passage of Plasmodium increases virulence, whilst mosquito transmission inherently regulates parasite virulence within the mammalian host. It is, therefore, imperative that all aspects of experimental malaria research are studied in the context of the complete Plasmodium life cycle.
  
  
  METHODS
  Plasmodium chabaudi chabaudi displays many characteristics associated with human Plasmodium infection of natural mosquito vectors and the mammalian host, and thus provides a unique opportunity to study the pathogenesis of malaria in a single infection setting. An optimized protocol that permits efficient and reproducible vector transmission of P. c. chabaudi via Anopheles stephensi was developed.
  
  
  CONCLUSIONS
  This protocol was utilized for mosquito transmission of genetically distinct P. c. chabaudi isolates, highlighting differential parasite virulence within the mosquito vector and the spectrum of host susceptibility to infection initiated via the natural route, mosquito bite. An apposite experimental system in which to delineate the pathogenesis of malaria is described in detail.
authors.affiliation: Division of Parasitology, MRC National Institute for Medical Research, Mill Hill, London NW7 1AA, UK. pspence@nimr.mrc.ac.uk
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Spence
authors.forename: William
authors.initials: W
authors.lastname: Jarra
authors.forename: Prisca
authors.initials: P
authors.lastname: Lévy
authors.forename: Wiebke
authors.initials: W
authors.lastname: Nahrendorf
authors.forename: Jean
authors.initials: J
authors.lastname: Langhorne
mesh_terms: D000818:Animals; D000852:Anopheles / Q000469:parasitology*; D004195:Disease Models, Animal; D004199:Disease Vectors; D004772:Entomology / Q000379:methods; D005260:Female; D008288:Malaria / Q000635:transmission*; D016788:Plasmodium chabaudi / Q000302:isolation & purification*; D012377:Rodentia; D014730:Veterinary Medicine / Q000379:methods