title: The relation of apple texture with cell wall nanostructure studied using an atomic force microscope.
abstract: In this study, the relation of the nanostructure of cell walls with their texture was investigated for six different apple cultivars. Cell wall material (CWM) and cellulose microfibrils were imaged by atomic force microscope (AFM). The mean diameter of cellulose microfibrils for each cultivar was estimated based on the AFM height topographs obtained using the tapping mode of dried specimens. Additionally, crystallinity of cellulose microfibrils and pectin content was determined. Texture of apple cultivars was evaluated by sensory and instrumental analysis. Differences in cellulose diameter as determined from the AFM height topographs of the nanostructure of cell walls of the apple cultivars are found to relate to the degree of crystallinity and pectin content. Cultivars with thicker cellulose microfibrils also revealed crisper, harder and juicier texture, and greater acoustic emission. The data suggest that microfibril thickness affects the mechanical strength of cell walls which has consequences for sensory and instrumental texture.
authors.affiliation: Institute of Agrophysics, Polish Academy of Sciences, Doswiadczalna 4, 20-290 Lublin, Poland. j.cybulska@ipan.lublin.pl
authors.forename: Justyna
authors.initials: J
authors.lastname: Cybulska
authors.forename: Artur
authors.initials: A
authors.lastname: Zdunek
authors.forename: Katarzyna M
authors.initials: KM
authors.lastname: Psonka-Antonczyk
authors.forename: Bjørn T
authors.initials: BT
authors.lastname: Stokke
mesh_terms: D002473:Cell Wall / Q000737:chemistry / Q000648:ultrastructure; D002482:Cellulose / Q000737:chemistry / Q000648:ultrastructure; D027845:Malus / Q000737:chemistry / Q000648:ultrastructure; D020894:Microfibrils / Q000737:chemistry / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D010368:Pectins / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D010368:Pectins; D002482:Cellulose