title: Endothelial barrier dysfunction in diabetic conduit arteries: a novel method to quantify filtration.
abstract: The endothelial barrier plays an important role in atherosclerosis, hyperglycemia, and hypercholesterolemia. In the present study, an accurate, reproducible, and user-friendly method was used to further understand endothelial barrier function of conduit arteries. An isovolumic method was used to measure the hydraulic conductivity (L(p)) of the intact vessel wall and medial-adventitial layer. Normal arterial segments with diameters from 0.2 to 5.5 mm were used to validate the method, and femoral arteries of diabetic rats were studied as an example of pathological specimens. Various arterial segments confirmed that the volume flux of water per unit surface area was linearly related to intraluminal pressure, as confirmed in microvessels. L(p) of the intact wall varied from 3.5 to 22.1 × 10(-7) cm·s(-1)·cmH(2)O(-1) over the pressure range of 7-180 mmHg. Over the same pressure range, L(p) of the endothelial barrier changed from 4.4 to 25.1 × 10(-7) cm·s(-1)·cmH(2)O(-1). During perfusion with albumin-free solution, L(p) of rat femoral arteries increased from 6.1 to 13.2 × 10(-7) cm·s(-1)·cmH(2)O(-1) over the pressure range of 10-180 mmHg. Hyperglycemia increased L(p) of the femoral artery in diabetic rats from 2.9 to 5.5 × 10(-7) cm·s(-1)·cmH(2)O(-1) over the pressure range of 20-135 mmHg. In conclusion, the L(p) of a conduit artery can be accurately and reproducibly measured using a novel isovolumic method, which in diabetic rats is hyperpermeable. This is likely due to disruption of the endothelial glycocalyx.
authors.affiliation: Biomedical Engineering, Indiana University-Purdue University, Indianapolis, Indiana 46202, USA.
authors.forename: Xiao
authors.initials: X
authors.lastname: Lu
authors.forename: Virginia H
authors.initials: VH
authors.lastname: Huxley
authors.forename: Ghassan S
authors.initials: GS
authors.lastname: Kassab
mesh_terms: D000465:Algorithms; D000818:Animals; D001158:Arteries / Q000503:physiopathology*; D050197:Atherosclerosis / Q000503:physiopathology; D001834:Body Water / Q000378:metabolism; D002196:Capillaries / Q000502:physiology; D003924:Diabetes Mellitus, Type 2 / Q000503:physiopathology*; D004730:Endothelium, Vascular / Q000502:physiology*; D005260:Female; D005263:Femoral Artery / Q000502:physiology; D005374:Filtration; D008297:Male; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D011924:Rats, Zucker; D013552:Swine