title: Ileal digestibility of amino acids in pea protein isolates, wheat-corn distillers dried grains with solubles, and short-season corn fed to broiler chicks.
abstract: The apparent ileal digestibility (AID) of amino acids (AA) in 4 pea protein isolates [PPI; experiment (Exp.) 1], 5 wheat- and corn-derived distillers grains with solubles (wcDDGS; Exp. 2), and 5 short-season corn (Exp. 3) samples for broiler chickens were determined. In addition, a reference wheat sample was included in each experiment to measure the repeatability of the ileal digestibility assay used. Birds (n = 180 for Exp. 1 and n = 216 for Exp. 2 or 3) were fed test diets from d 15 to 21 of age. Reference wheat and PPI were included in test diets at 91.7 and 25%, respectively, as the sole source of AA, whereas wcDDGS and corn were combined with wheat in test diets at 47.7% test ingredient (wcDDGS or corn) and 51% wheat as the sources of AA. Chromic oxide at 0.3% was added in all diets as the indigestible marker. Each test diet was randomly assigned to 6 cages of 6 male birds each. On d 21, birds were killed and ileal digesta were collected to determine the AID of AA. The mean AID values for indispensable AA ranged from 86.0% (Thr) to 94.7% (Arg) for PPI, 50.7% (Lys) to 79.8% (Leu) for wcDDGS, and 73.9% (Thr) to 95.3% (Arg) for corn. Overall, the CV range for AID of AA was widest for wcDDGS (8.1% for Met to 29.4% for Lys) followed by corn (3.0% for Met to 10.7% for Thr) and then PPI (2.7% for Lys to 6.1% for Thr). For wcDDGS and corn, the within-sample variation (assay condition) was more than between-sample variation (feed differences), whereas the reverse was true for PPI. No differences were detected in the AID of CP and AA for reference wheat among experiments.
authors.affiliation: Department of Animal Science, University of Manitoba, Winnipeg, Manitoba, Canada, R3T 2N2.
authors.forename: N K
authors.initials: NK
authors.lastname: Nandha
authors.forename: T A
authors.initials: TA
authors.lastname: Woyengo
authors.forename: R L
authors.initials: RL
authors.lastname: Payne
authors.forename: C M
authors.initials: CM
authors.lastname: Nyachoti
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism*; D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002645:Chickens / Q000378:metabolism*; D004032:Diet / Q000662:veterinary; D004044:Dietary Proteins / Q000032:analysis / Q000378:metabolism; D004063:Digestion / Q000502:physiology; D007082:Ileum / Q000378:metabolism; D008297:Male; D018532:Pisum sativum / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D014908:Triticum / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D004044:Dietary Proteins; D010940:Plant Proteins