title: Simple paper architecture modifications lead to enhanced sensitivity in nanoparticle based lateral flow immunoassays.
abstract: Lateral flow immunoassays (LFIA) are ideal biosensors to detect proteins, but their lack of sensitivity hinders their extensive use. We report a strategy that yields up to an 8-fold improvement in the sensitivity of a gold nanoparticles-based LFIA by changing the sizes of the pads. Theoretical flow simulations of the developed LFIA architectures are in accordance with the experimental results.
authors.affiliation: Nanobioelectronics & Biosensors Group, Institut Català de Nanotecnologia, CIN2 (ICN-CSIC), Campus UAB, Barcelona, Spain.
authors.forename: Claudio
authors.initials: C
authors.lastname: Parolo
authors.forename: Mariana
authors.initials: M
authors.lastname: Medina-Sánchez
authors.forename: Alfredo
authors.initials: A
authors.lastname: de la Escosura-Muñiz
authors.forename: Arben
authors.initials: A
authors.lastname: Merkoçi
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D003198:Computer Simulation; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007074:Immunoglobulin G / Q000032:analysis; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008962:Models, Theoretical
chemical_list: D007074:Immunoglobulin G; D006046:Gold