title: Hyperspectral multimodal CARS microscopy in the fingerprint region.
abstract: A simple scheme for multimodal coherent anti-Stokes Raman scattering (CARS) microscopy is based on the spectral focusing of ultrafast-oscillator-derived pump/probe light and synchronous photonic crystal fiber (PCF) fiber-generated broadband Stokes light. To date, such schemes allowed rapid hyperspectral imaging throughout the CH/OH high frequency region (2700-4000 cm(-1) ). Here we extend this approach to the middle (1640-3300 cm(-1) ) and fingerprint regions (850-1800 cm(-1) ) of the Raman spectrum. Our simple integrated approach to rapid hyperspectral CARS microscopy in the fingerprint region is demonstrated by applications to label-free multimodal imaging of cellulose and bulk bone, including use of the phosphate resonance at 960 cm(-1) .
authors.affiliation: Department of Physics, Queen's University, Kingston, Ontario, K7L 3N6 Canada; National Research Council of Canada, Ottawa, Ontario, K1A 0A6 Canada.
authors.forename: Adrian F
authors.initials: AF
authors.lastname: Pegoraro
authors.forename: Aaron D
authors.initials: AD
authors.lastname: Slepkov
authors.forename: Andrew
authors.initials: A
authors.lastname: Ridsdale
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Moffatt
authors.forename: Albert
authors.initials: A
authors.lastname: Stolow
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D015519:Bone Density; D002417:Cattle; D002482:Cellulose / Q000737:chemistry; D003094:Collagen / Q000378:metabolism; D008853:Microscopy / Q000379:methods*; D009578:Nitrobenzenes / Q000737:chemistry; D012272:Ribs / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D013059:Spectrum Analysis, Raman; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D009578:Nitrobenzenes; D014867:Water; D002482:Cellulose; D003094:Collagen