title: Antibacterial, antioxidant and cytotoxic studies of total saponin, alkaloid and sterols contents of decoction of Joshanda: identification of components through thin layer chromatography.
abstract: The current study was aimed to assess antibacterial, antioxidant and cytotoxic activity of total saponin, alkaloid and sterol contents of Joshanda decoction followed by its constituent's analysis via thin layer chromatography (TLC). Saponins and alkaloids showed prominent antibacterial activity against Staphylococcus aureus, Bacillus subtilis, Bacillus cereus and Klebsiella pneumoniae whereas sterols only against S. aureus. Saponin and alkaloid contents of 97 and 108 µg/ml, respectively, showed prominent free radical scavenging activity against 1,1-diphenyl-2-picrylhidrazyl, with mild cytotoxicity in brine shrimp cytotoxic test. Under ultraviolet light at 254 nm, TLC of total saponins showed eight different compounds, total sterols comprising three while total alkaloids two compounds of various polarities. It is concluded that the various contents of Joshanda decoction possess outstanding susceptibility against bacteria implicating primarily upper respiratory tract infections augmented by strong antioxidant activity.
authors.affiliation: Gandhara College of Pharmacy, Gandhara University, Peshawar, Pakistan Department of Pharmacy, Abdul Wali Khan University, Mardan, Pakistan harronkhan3@yahoo.com.
authors.forename: Haroon
authors.initials: H
authors.lastname: Khan
authors.affiliation: Department of Chemistry, Kohat University of Science and Technology, Kohat, Pakistan.
authors.forename: Murad Ali
authors.initials: MA
authors.lastname: Khan
authors.affiliation: Department of Chemistry, Kohat University of Science and Technology, Kohat, Pakistan.
authors.lastname: Abdullah
mesh_terms: D000470:Alkaloids / Q000032:analysis / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects; D002855:Chromatography, Thin Layer; D003603:Cytotoxins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007711:Klebsiella pneumoniae / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D012503:Saponins / Q000302:isolation & purification* / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects; D013261:Sterols / Q000032:analysis / Q000494:pharmacology
chemical_list: D000470:Alkaloids; D000900:Anti-Bacterial Agents; D000975:Antioxidants; D003603:Cytotoxins; D010936:Plant Extracts; D012503:Saponins; D013261:Sterols