title: The TRIMCyp genotype in four species of macaques in China.
abstract: The tripartite motif protein (TRIM)5α/CypA fusion protein TRIMCyp in Old World monkeys is generally considered unable to restrict HIV-1 replication. Monkeys with TRIMCyp can serve as a unique animal model for studies of HIV-1 infection. The present study investigated the distribution and expression status of TRIMCyp in four species of macaques originating from China and its borderlands: pigtail macaques (Macaca nemestrina), rhesus macaques (Macaca mulatta), long-tailed macaques (Macaca fascicularis), and Tibetan macaques (Macaca thibetana). The results revealed that the frequencies of the TRIMCyp genotype were significantly different among different species and even within different populations of the same species. Interestingly, the TRIMCyp genotype was more prevalent among macaques originating from Yunnan and surrounding regions than those from other regions of China. Importantly, TRIMCyp individuals were first identified in Chinese M. mulatta originating from Yunnan, although multiple earlier studies failed to find CypA retrotransposition in this subspecies. Furthermore, TRIMe7-CypA, one of the splicing isoforms of the TRIMCyp transcript was expressed in M. nemestrina and M. mulatta but not M. fascicularis. The intra- and interspecies polymorphisms in the deduced TRIMCyp amino acid sequences of these macaques were also analyzed. Taken together, the data in this study provide important information about the genomic background of TRIMCyp among major species of Chinese macaques.
authors.affiliation: Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Yu
authors.forename: Lei
authors.initials: L
authors.lastname: Na
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Lv
authors.forename: Jian-Dong
authors.initials: JD
authors.lastname: Liu
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Ji
authors.forename: Yong-Hui
authors.initials: YH
authors.lastname: Zheng
authors.forename: Hong-Li
authors.initials: HL
authors.lastname: Du
authors.forename: Xian-Gang
authors.initials: XG
authors.lastname: Kong
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Zhou
mesh_terms: D063147:Animal Distribution; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002681:China; D060467:Disease Resistance / Q000235:genetics; D016368:Frameshift Mutation; D005838:Genotype; D015658:HIV Infections / Q000235:genetics; D015497:HIV-1; D008251:Macaca / Q000235:genetics*; D008252:Macaca fascicularis / Q000235:genetics; D008253:Macaca mulatta / Q000235:genetics; D008254:Macaca nemestrina / Q000235:genetics; D008969:Molecular Sequence Data; D050506:Mutant Chimeric Proteins / Q000235:genetics*; D011110:Polymorphism, Genetic; D020033:Protein Isoforms / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D011544:Pseudogenes; D018626:Retroelements / Q000235:genetics*; D013045:Species Specificity; D044767:Ubiquitin-Protein Ligases
chemical_list: D002352:Carrier Proteins; D050506:Mutant Chimeric Proteins; D020033:Protein Isoforms; D011506:Proteins; D018626:Retroelements; C526560:TRIM5-cyclophilin A protein, Macaca nemestrina; C482587:TRIM5(alpha) protein, rhesus monkey; D044767:Ubiquitin-Protein Ligases