title: Radio frequency induced hyperthermia mediated by dextran stabilized LSMO nanoparticles: in vitro evaluation of heat shock protein response.
abstract: Dextran stabilized La(0.7)Sr(0.3)MnO(3) (Dex-LSMO) is an alternative cancer hyperthermia agent holding considerable promise. Here, we have carried out a comparative study on radio frequency (~264 kHz) induced Dex-LSMO mediated heating and extraneous heating (mimicking generalized hyperthermia) in terms of changes in the morphology, proliferation pattern and induction of heat shock proteins in a human melanoma cell line (A375). Our results clearly show that the cellular effects seen with extraneous heating (60 min at 43 °C) could be reproduced by just six minutes of radio frequency induced Dex-LSMO mediated heating. More importantly, the observed enhanced levels of HSP 70 and 90 (molecular markers of heat shock that trigger favorable immunological reactions) seen with Dex-LSMO mediated heating were comparable to extraneous heating. These results suggest the possible utility of Dex-LSMO as a cancer hyperthermia agent.
authors.affiliation: Centre for Nanobioscience, Agharkar Research Institute, G G Agarkar Road, Pune 411004, India.
authors.forename: K R
authors.initials: KR
authors.lastname: Bhayani
authors.forename: J M
authors.initials: JM
authors.lastname: Rajwade
authors.forename: K M
authors.initials: KM
authors.lastname: Paknikar
mesh_terms: D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D003911:Dextrans / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism*; D018869:Heat-Shock Response / Q000187:drug effects*; D006801:Humans; D006979:Hyperthermia, Induced; D007811:Lanthanum / Q000633:toxicity; D017895:Manganese Compounds; D053768:Metal Nanoparticles / Q000633:toxicity*; D008858:Microscopy, Phase-Contrast; D010087:Oxides / Q000633:toxicity; D011846:Radio Waves; D013324:Strontium / Q000633:toxicity; D013997:Time Factors
chemical_list: D003911:Dextrans; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D017895:Manganese Compounds; D010087:Oxides; C027424:manganese oxide; D007811:Lanthanum; D013324:Strontium