title: Human TNF cytokine neutralization with a vNAR from Heterodontus francisci shark: a potential therapeutic use.
abstract: The therapeutic use of single domain antibodies (sdAbs) is a promising new approach because these small antibodies maintain antigen recognition and neutralization capacity, have thermal and chemical stability and have good solubility. In this study, using phage display technology, we isolated a variable domain of a IgNAR (vNAR) from a Heterodontus francisci shark immunized against the recombinant human cytokine TNFα (rhTNFα). One clone T43, which expresses the vNAR protein in the periplasmic space, was isolated from the fourth round of panning. T43 had the capacity to recognize rhTNF and neutralize it in vitro, indicating that T43 has potential as a therapeutic that can be used for diseases in which this pro-inflammatory cytokine needs to be controlled.
authors.affiliation: Marine Science Faculty, Universidad Autonoma de Baja California, Ensenada Baja California, Mexico.
authors.forename: Tanya
authors.initials: T
authors.lastname: Camacho-Villegas
authors.forename: Teresa
authors.initials: T
authors.lastname: Mata-Gonzalez
authors.forename: Jorge
authors.initials: J
authors.lastname: Paniagua-Solis
authors.forename: Edna
authors.initials: E
authors.lastname: Sanchez
authors.forename: Alexei
authors.initials: A
authors.lastname: Licea
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D057134:Antibodies, Neutralizing / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology* / Q000627:therapeutic use; D000918:Antibody Specificity; D006801:Humans; D007114:Immunization; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology; D007136:Immunoglobulins / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D019151:Peptide Library; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012754:Sharks / Q000276:immunology*; D012772:Shock, Septic / Q000276:immunology; D061905:Single-Domain Antibodies / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology* / Q000627:therapeutic use; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D007135:Immunoglobulin Variable Region; D007136:Immunoglobulins; D019151:Peptide Library; D011994:Recombinant Proteins; D061905:Single-Domain Antibodies; D014409:Tumor Necrosis Factor-alpha