title: Inhibitor of apoptosis protein-like protein-2 as a novel serological biomarker for breast cancer.
abstract: Inhibitor of apoptosis protein-like protein-2 (ILP-2) has only been detected in the testis and in lymphoblastoid cells. Although previous studies have not reported the presence of ILP-2 in breast cancer tissues, this study indicates the presence of ILP-2 in breast cancer serum samples. To validate whether ILP-2 is a novel serological biomarker for breast cancer, we conducted two-dimensional gel electrophoresis (2DE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry analysis on 400 breast cancer serum samples and 40 non-cancer serum samples (i.e., healthy controls). We then performed a Western blot analysis of 10 breast cancer serum samples and 10 non-cancer serum samples. Finally, we analyzed 35 serum samples from healthy controls or subjects with breast cancer, other types of cancer, galactophore hyperplasia or breast cancer post-surgery by using 2DE and enzyme-linked immunosorbent assay. Our results indicate that ILP-2 is a novel breast cancer biomarker in the peripheral blood.
authors.affiliation: Department of Biochemistry & Molecular Biology, College of Medical Science, Jishou University, Jishou 416000, China. xmj688@163.com.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Xiang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Dandan
authors.initials: D
authors.lastname: Gao
authors.forename: Xiansong
authors.initials: X
authors.lastname: Fang
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood*; D001943:Breast Neoplasms / Q000097:blood*; D005260:Female; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000097:blood*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000097:blood*
chemical_list: C430870:BIRC8 protein, human; D014408:Biomarkers, Tumor; D051034:Inhibitor of Apoptosis Proteins; D009363:Neoplasm Proteins