title: CEACAM1 inhibits cell-matrix adhesion and promotes cell migration through regulating the expression of N-cadherin.
abstract: Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is a member of the immunoglobulin super family and has been observed to have two paradoxical functions: tumor suppression and the promotion of tumor invasion. In the present study, we discovered that CEACAM1 functions as an adhesion inhibitor and a migration promoter. The CEACAM1 transfected cells, either 293-CEACAM1 or LOVO/trans-CEACAM1, was proved to have lower adhesion rate. Furthermore, HT29/siRNA-CEACAM1 cells had a higher adhesion rate than HT29 cells. These results indicated that CEACAM1 was an inhibitor of cell-matrix adhesion. Additionally, 293-CEACAM1 LOVO/trans-CEACAM1 cells exhibited better motility in a trans-well migration assay. N-cadherin expression levels were positively correlated with CEACAM1 in 293-CEACAM1, LOVO/trans-CEACAM1 and HT29/siRNA-CEACAM1 cells. When blocked by a GC-4 antibody, the adhesive capacities of 293-CEACAM1 and LOVO/trans-CEACAM1 were recovered and the motilities of them were suppressed, which suggested that CEACAM1 functioned through N-cadherin.
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.forename: Guohu
authors.initials: G
authors.lastname: Di
authors.forename: Chu-Tse
authors.initials: CT
authors.lastname: Wu
authors.forename: Xianwen
authors.initials: X
authors.lastname: Hu
authors.forename: Haifeng
authors.initials: H
authors.lastname: Duan
mesh_terms: D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D015820:Cadherins / Q000096:biosynthesis*; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement; D057809:HEK293 Cells; D019073:HT29 Cells; D006801:Humans; D008954:Models, Biological; D009362:Neoplasm Metastasis / Q000473:pathology*
chemical_list: D015703:Antigens, CD; C069847:CD66 antigens; D015820:Cadherins; D015815:Cell Adhesion Molecules