title: miR‑142‑3p promotes osteoblast differentiation by modulating Wnt signaling.
abstract: Canonical Wnt signaling is critical for the control of osteoblast differentiation in human mesenchymal stem cells. MicroRNAs (miRs) are essential regulators of cell differentiation by post‑transcriptional regulation of target gene expression. The aim of the present study was to investigate the molecular mechanism by which miR‑142‑3p promotes osteoblastic differentiation using the human fetal osteoblastic 1.19 (hFOB1.19), real-time PCR and western blot analysis. Results showed an increased expression of miR‑142‑3p during osteoblast differentiation in the mesenchymal precursor cell line, hFOB1.19. In addition, the ectopic over-expression of miR‑142‑3p promoted hFOB1.19 differentiation, whereas the inhibition of miR‑142‑3p repressed differentiation. The expression of miR‑142‑3p was positively correlated with β‑catenin, an important protein in Wnt signaling. The adenomatous polyposis coli (APC) gene was a direct target of miR‑142‑3p, whereby miR‑142‑3p promoted Wnt signaling through inhibition of APC, leading to accumulation and nuclear translocation of β‑catenin. Therefore, miR‑142‑3p may be an essential mediator of osteoblast differentiation and a new therapeutic strategy for osteogenesis disorders.
authors.affiliation: Department of Orthopedics, Tongji Hospital, Tongji Medical College,                     Huazhong University of Science and Technology, Wuhan 430030, P.R. China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Hu
authors.forename: Yaping
authors.initials: Y
authors.lastname: Ye
authors.forename: Weikai
authors.initials: W
authors.lastname: Zhang
authors.forename: Jiang
authors.initials: J
authors.lastname: Wang
authors.forename: Anmin
authors.initials: A
authors.lastname: Chen
authors.forename: Fengjing
authors.initials: F
authors.lastname: Guo
mesh_terms: D020413:3' Untranslated Regions; D025601:Adenomatous Polyposis Coli Protein / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002454:Cell Differentiation; D002460:Cell Line; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis; D015398:Signal Transduction; D015854:Up-Regulation; D051153:Wnt Proteins / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D025601:Adenomatous Polyposis Coli Protein; C555159:MIRN142 microRNA, human; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; D051153:Wnt Proteins; D051176:beta Catenin