title: The experimental study of Astragalus membranaceus on meridian tropsim: the distribution study of astragaloside IV in rat tissues.
abstract: According to Traditional Chinese Medicine (TCM) theories, TCM with different meridian tropism have different therapeutic effects. In view of the meridian tropism of Astragalus membranaceus (Huangqi), astragaloside IV, one of the effective phytochemicals of Huangqi, was appointed and observed its distribution in rat tissues following a single intravenous (i.v.) dose. A simple and accurate LC-ESI-MS/MS method was developed and validated for astragaloside IV quantification in heart, liver, spleen, lung and kidney using warfarin as an internal standard (IS). Chromatographic separation was performed on a Eclipse plus C18 (4.6mm×100mm, 1.8μm) when the flow rate was set at 0.300mLmin(-1) and ammonium acetate aqueous solution - acetonitrile was used as mobile phase. The intra- and inter-day precisions of the quality control samples were within 15% and accuracies were within 90.0-110%. The recoveries were more than 90.0% at high, medium and low concentrations, respectively. This method was successfully applied for distribution of astragaloside IV after intravenous (i.v.) dose of 4mgkg(-1) astragaloside IV in rats. Astragaloside IV concentration was highest in liver and kidney and remained much higher than that in other tissues over the experiment course. Lung, heart and spleen were also detected to contain astragaloside IV. The results clearly demonstrated that astragaloside IV was one of the material bases of the meridian tropism of Huangqi.
authors.affiliation: Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China. tcmcyx@126.com
authors.forename: Yan-Xu
authors.initials: YX
authors.lastname: Chang
authors.forename: Yu-Gang
authors.initials: YG
authors.lastname: Sun
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Qiu-Hong
authors.initials: QH
authors.lastname: Zhang
authors.forename: Xin-Rong
authors.initials: XR
authors.lastname: Guo
authors.forename: Bo-Li
authors.initials: BL
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Jin
authors.forename: Xiu-Mei
authors.initials: XM
authors.lastname: Gao
mesh_terms: D000818:Animals; D027885:Astragalus propinquus / Q000737:chemistry / Q000502:physiology*; D002853:Chromatography, Liquid / Q000379:methods; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000493:pharmacokinetics*; D016014:Linear Models; D008168:Lung / Q000737:chemistry / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000032:analysis / Q000493:pharmacokinetics*; D013154:Spleen / Q000737:chemistry / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D014018:Tissue Distribution; D014315:Triterpenes / Q000032:analysis / Q000493:pharmacokinetics*; D018523:Tropism / Q000502:physiology
chemical_list: D004365:Drugs, Chinese Herbal; D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A