title: Synthetic nanoparticles functionalized with biomimetic leukocyte membranes possess cell-like functions.
abstract: The therapeutic efficacy of systemic drug-delivery vehicles depends on their ability to evade the immune system, cross the biological barriers of the body and localize at target tissues. White blood cells of the immune system--known as leukocytes--possess all of these properties and exert their targeting ability through cellular membrane interactions. Here, we show that nanoporous silicon particles can successfully perform all these actions when they are coated with cellular membranes purified from leukocytes. These hybrid particles, called leukolike vectors, can avoid being cleared by the immune system. Furthermore, they can communicate with endothelial cells through receptor-ligand interactions, and transport and release a payload across an inflamed reconstructed endothelium. Moreover, leukolike vectors retained their functions when injected in vivo, showing enhanced circulation time and improved accumulation in a tumour.
authors.affiliation: Department of Nanomedicine, The Methodist Hospital System Research Institute, Houston, Texas 77030, USA.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Parodi
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Quattrocchi
authors.forename: Anne L
authors.initials: AL
authors.lastname: van de Ven
authors.forename: Ciro
authors.initials: C
authors.lastname: Chiappini
authors.forename: Michael
authors.initials: M
authors.lastname: Evangelopoulos
authors.forename: Jonathan O
authors.initials: JO
authors.lastname: Martinez
authors.forename: Brandon S
authors.initials: BS
authors.lastname: Brown
authors.forename: Sm Z
authors.initials: SZ
authors.lastname: Khaled
authors.forename: Iman K
authors.initials: IK
authors.lastname: Yazdi
authors.forename: Maria Vittoria
authors.initials: MV
authors.lastname: Enzo
authors.forename: Lucas
authors.initials: L
authors.lastname: Isenhart
authors.forename: Mauro
authors.initials: M
authors.lastname: Ferrari
authors.forename: Ennio
authors.initials: E
authors.lastname: Tasciotti
mesh_terms: D000818:Animals; D001692:Biological Transport; D032701:Biomimetics / Q000379:methods*; D002448:Cell Adhesion; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007962:Leukocytes / Q000737:chemistry* / Q000378:metabolism; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000737:chemistry / Q000378:metabolism; D008567:Membranes, Artificial; D051379:Mice; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D053758:Nanoparticles / Q000737:chemistry*; D010587:Phagocytosis
chemical_list: D008567:Membranes, Artificial