title: [Determination of donepezil enantiomer in human plasma by normal-HPLC-MS/MS].
abstract: OBJECTIVE
  To develop and validate a normal phase HPLC-MS/MS method for the determination of donepezil enantiomer in human plasma.
  
  
  METHODS
  Donepezil was extracted from plasma by n-hexane:isopropanol (98:2, V/V) with lidocaine serving as an internal standard. The analytes went through the column of CHIRALCEL OJ-H (250 mm x 4.6 mm, 5 microm) with mobile phase n-hexane:n-propanol:diethylamine (60:40: 0.1, V/V/V). Donepezil enantiomer was determined by API 3000 in MRM mode.
  
  
  RESULTS
  The retention time of S-DN and R-DN were 15.56 min and 18.41 min, respectively. The calibration curves were linear in a range from 0.051 to 7.596 ng/mL for S-DN, and from 0.049 to 7.404 ng/mL for R-DN, respectively, both with more than 0.99 correlation coefficients. The relative recovery were 95.10%-103.70% for S-DN and 93.58%-98.00% for R-DN, respectively; the pretreatment recovery were 58.42%-61.08% for S-DN and 53.24%-61.87% for R-DN, respectively; the within-day RSD ranged from 8.35% to 11.28% for S-DN and from 6.78% to 11.58% for R-DN, respectively; the between-day RSD ranged from 5.82% to 9.02% for S-DN and from 6.87% to 9.19% for R-DN, respectively.
  
  
  CONCLUSIONS
  This normal phase HPLC-MS/MS method is simple, rapid, sensitive and accurate for the determination of donepezil enantiomer in human plasma and is suitable for pharmacokinetic studies of donepezil enantiomers.
authors.affiliation: Department of Clinical Pharmacology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yu
authors.forename: Jin
authors.initials: J
authors.lastname: Xiang
authors.forename: Ling-Zi
authors.initials: LZ
authors.lastname: Shi
authors.forename: Mao-Zhi
authors.initials: MZ
authors.lastname: Liang
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Qin
authors.forename: Feng
authors.initials: F
authors.lastname: Nan
mesh_terms: D002800:Cholinesterase Inhibitors / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000077265:Donepezil; D006801:Humans; D007189:Indans / Q000097:blood*; D010880:Piperidines / Q000097:blood*; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D002800:Cholinesterase Inhibitors; D007189:Indans; D010880:Piperidines; D000077265:Donepezil