title: Anti-inflammatory and antinociceptive effects of 6-(4-chlorophenoxy)-tetrazolo[5,1-a]phthalazine in mice.
abstract: BACKGROUND
  QUAN-0808 (6-(4-chlorophenoxy)-tetrazolo[5,1-a]phthalazine), a new phthalazine tetrazole derivative, was evaluated for the anti-inflammatory and analgesic effects.
  
  
  METHODS
  Xylene-induced ear edema, carrageenan (Carr)-induced paw edema, and acetic acid-induced capillary permeability hyperactivity in mice were used to assess the anti-inflammatory effect; acetic acid-induced writhing and hot plate responses for the analgesic activity.
  
  
  RESULTS
  In the present study, QUAN-0808 (100, 200, 400 mg/kg) and indomethacin (Indo) significantly decreased xylene-induced ear edema by 33.3, 37.5, 46.6, and 45.1%, respectively, decreased Carr-induced paw edema at 1, 2, 4 h after Carr injection, and decreased the prostaglandin E(2) (PGE(2)) and nitric oxide (NO) levels on the edema paw at 4 h after Carr injection; QUAN-0808 (100, 200, 400 mg/kg), and aspirin (Asp, 200 mg/kg) significantly decreased Evans blue exudation in acetic acid-induced capillary permeability hyperactivity model by 26.7, 28.7, 32.3 and 29.1%, respectively, and decreased the numbers of acetic acid-induced writhing response in 15 min by 40.4, 53.6, 66.4, and 64.5%, respectively. Morphine (10 mg/kg) significantly increased the latency of the hot plate response by 136.5, 117.4, 67.5, and 22.7%, respectively, at 30, 60, 90, 120 min after intraperitoneal injection of morphine; however, QUAN-0808 (100, 200 and 400 mg/kg) did not produce significantly antinociceptive effects in the hot plate test, suggesting that its antinociceptive action occurs via peripheral rather than a central-acting mechanism.
  
  
  CONCLUSIONS
  These results show that QUAN-0808 produced potential anti-inflammatory and peripheral antinociceptive effects, and indicated that the antinociceptive effects of QUAN-0808 were related to its anti-inflammatory activity in a dose-dependent manner. Therefore, as inflammation is a peripheral process, it is suggested that QUAN-0808 exerted peripheral effects. The peripheral effect mechanisms of QUAN-0808 may be related to a decrease in the production of PGE(2), NO, bradykinin and other inflammatory mediators.
authors.affiliation: College of Based Medicine, Yanbian University, Yanji, Jilin, PR China.
authors.forename: Hai-Ling
authors.initials: HL
authors.lastname: Yu
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Ying-Jun
authors.initials: YJ
authors.lastname: Li
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Gong
authors.forename: Zhe-Shan
authors.initials: ZS
authors.lastname: Quan
mesh_terms: D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D015232:Dinoprostone / Q000032:analysis; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000502:physiology; D010538:Peritonitis / Q000188:drug therapy; D010793:Phthalazines / Q000494:pharmacology*; D013777:Tetrazoles / Q000494:pharmacology*; D014992:Xylenes / Q000494:pharmacology
chemical_list: C552309:6-(4-chlorophenoxy)tetrazolo(5,1-a)phthalazine; D000700:Analgesics; D000893:Anti-Inflammatory Agents; D010793:Phthalazines; D013777:Tetrazoles; D014992:Xylenes; D009569:Nitric Oxide; D015232:Dinoprostone