title: Evernia prunastri and Pseudoevernia furfuraceae lichens and their major metabolites as antioxidant, antimicrobial and anticancer agents.
abstract: The aim of this study is to investigate chemical composition of acetone extracts of the lichens Evernia prunastri and Pseudoevernia furfuraceae and in vitro antioxidant, antimicrobial, and anticancer activities of these extracts and some their major metabolites. HPLC-UV method was used for identification of secondary metabolites. Antioxidant activity was evaluated by free radical scavenging, superoxide anion radical scavenging, reducing power and determination of total phenolic compounds. As a result of the study physodic acid had largest antioxidant activities. Total content of phenol in extracts was determined as pyrocatechol equivalent. The antimicrobial activity was estimated by determination of the minimal inhibitory concentration by the broth microdilution method. The most active was also physodic acid. Anticancer activity was tested against FemX (human melanoma) and LS174 (human colon carcinoma) cell lines using MTT method.
authors.affiliation: Department of Biology, Faculty of Science, University of Kragujevac, 34000 Kragujevac, Serbia.
authors.forename: Marijana
authors.initials: M
authors.lastname: Kosanić
authors.forename: Nedeljko
authors.initials: N
authors.lastname: Manojlović
authors.forename: Slobodan
authors.initials: S
authors.lastname: Janković
authors.forename: Tatjana
authors.initials: T
authors.lastname: Stanojković
authors.forename: Branislav
authors.initials: B
authors.lastname: Ranković
mesh_terms: D000096:Acetone / Q000378:metabolism; D000890:Anti-Infective Agents / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001203:Ascomycota / Q000378:metabolism*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D003110:Colonic Neoplasms / Q000473:pathology; D006801:Humans; D008011:Lichens / Q000737:chemistry / Q000378:metabolism*; D008545:Melanoma / Q000473:pathology; D008826:Microbial Sensitivity Tests; D010636:Phenols / Q000494:pharmacology; D013481:Superoxides
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D000975:Antioxidants; D010636:Phenols; D013481:Superoxides; D000096:Acetone