title: [DNA identification of Zijingpi's adulterant species Schisandra sphenanthera based on NCBI nucleotide database analysis].
abstract: OBJECTIVE
  To provide basis for quality control of Zijingpi, DNA identification was used based on NCBI nucleotide database analysis.
  
  
  METHODS
  Firstly, total DNA of Zijingpi was extracted. Secondly, the ITS sequence was amplified by PCR with universal primer of ITS and PCR products was directly sequenced after purification. Finally, ITS sequence similarity and phylogenetic tree were used for identification.
  
  
  RESULTS
  The ITS sequence information of the mainstream commercial drugs of Zijingpi was obtained.
  
  
  CONCLUSIONS
  It is firstly reported that the mainstream commercial drugs of Zijingpi was the bark of Schisandra sphenanthera.
authors.affiliation: School of Chinese Materia Medica, Beijing University of Traditional Chinese Medicine, Beiing 100102, China. 7676508@qq.com
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Cheng
authors.forename: Cai-li
authors.initials: CL
authors.lastname: Liao
authors.forename: Chun-sheng
authors.initials: CS
authors.lastname: Liu
authors.forename: Zhen-fang
authors.initials: ZF
authors.lastname: Bai
authors.forename: Yao-jun
authors.initials: YJ
authors.lastname: Yang
authors.forename: Jian
authors.initials: J
authors.lastname: Zheng
authors.forename: Ji
authors.initials: J
authors.lastname: Zhang
mesh_terms: D018744:DNA, Plant / Q000235:genetics; D030561:Databases, Nucleic Acid; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D008969:Molecular Sequence Data; D010802:Phylogeny; D011786:Quality Control; D032141:Schisandra / Q000145:classification* / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D018744:DNA, Plant; D004365:Drugs, Chinese Herbal