title: Comparative quantitative analysis of gene expression profiles of glycoside hydrolase family 10 xylanases in the sheep rumen during a feeding cycle.
abstract: Xylanase is a crucial hydrolytic enzyme that degrades plant polysaccharides in the rumen. To date, there is no information on the genetic composition and expression characteristics of ruminal xylanase during feeding cycles of ruminants. Here, the major xylanase of the glycoside hydrolase family 10 (GH 10) from the rumen of small-tail Han sheep was investigated during a feeding cycle. We identified 44 distinct GH 10 xylanase gene fragments at both the genomic and transcriptional levels. Comparison of their relative abundance showed that results from the evaluation of functional genes at the transcriptional level are more reliable indicators for understanding fluctuations in xylanase levels. The expression patterns of six xylanase genes, detected at all time points of the feeding cycle, were investigated; we observed a complex trend of gene expression over 24 h, revealing the dynamic expression of xylanases in the rumen. Further correlation analysis indicated that the rumen is a dynamic ecosystem where the transcript profiles of xylanase genes are closely related to ruminal conditions, especially rumen pH and bacterial population. Given the huge diversity and changing composition of enzymes over the entire rumen, this research provides valuable information for understanding the role of functional genes in the digestion of plant material.
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Li
authors.forename: Heng
authors.initials: H
authors.lastname: Zhao
authors.forename: Peilong
authors.initials: P
authors.lastname: Yang
authors.forename: Junqi
authors.initials: J
authors.lastname: Zhao
authors.forename: Huoqing
authors.initials: H
authors.lastname: Huang
authors.forename: Xianli
authors.initials: X
authors.lastname: Xue
authors.forename: Xinshang
authors.initials: X
authors.lastname: Zhang
authors.forename: Qiyu
authors.initials: Q
authors.lastname: Diao
authors.forename: Bin
authors.initials: B
authors.lastname: Yao
mesh_terms: D000818:Animals; D004032:Diet / Q000379:methods*; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000235:genetics; D054892:Metagenome; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000378:metabolism*; D012417:Rumen / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012756:Sheep; D059467:Transcriptome
chemical_list: D011134:Polysaccharides; D006026:Glycoside Hydrolases