title: Two novel SNPs of the 3-hydroxy-3-methylglutaryl coenzyme A reductase gene associated  with growth and meat quality traits in  the chicken.
abstract: The enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) is rate-limiting for metabolism of cholesterol; it plays an important role in endogenous cholesterol biosynthesis. We used DNA sequencing technology and created restriction site PCR-RFLP to detect HMGCR SNPs in an F(2) resource population of Gushi chicken and Anka broilers. We found a G/T mutation (Gln/His) in exon 17 and a T/C mutation (Pro/Pro) in exon 18. Based on association analysis of these HMGCR polymorphisms in 864 Gushi/Anka F(2) hybrids, these two mutations have significant effects on growth, carcass, meat quality, and lipid concentration.
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China. weiyang614@163.com
authors.forename: Y
authors.initials: Y
authors.lastname: Wei
authors.forename: S K
authors.initials: SK
authors.lastname: Zhu
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: R L
authors.initials: RL
authors.lastname: Han
authors.forename: Y D
authors.initials: YD
authors.lastname: Tian
authors.forename: G R
authors.initials: GR
authors.lastname: Sun
authors.forename: X T
authors.initials: XT
authors.lastname: Kang
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics*; D001835:Body Weight / Q000235:genetics; D002645:Chickens / Q000235:genetics* / Q000254:growth & development; D063427:Food Quality; D005787:Gene Frequency; D056726:Genetic Association Studies / Q000662:veterinary; D005828:Genetics, Population; D006579:Heterozygote; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics*; D050356:Lipid Metabolism; D008460:Meat / Q000592:standards*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
chemical_list: D030161:Avian Proteins; D006903:Hydroxymethylglutaryl CoA Reductases