title: The expression of non-mast histamine in tumor associated microvessels in human colorectal cancers.
abstract: Angiogenesis is essential for the growth, expansion and metastasis of human colorectal cancers (CRCs). Histamine produced by mast cells is a potent proangiogenic factor. However, the significance of non-mast cell expressing histamine in the tumor microenvironment remains unknown. In this study, we evaluated the histamine positive microvessels with the specific marker for biosynthesis of histamine L-histidine decarboxylase (HDC) in the CRC tumor microenvironment. The relationship between HDC positive microvessel density (HDC-MVD) and clinical pathological parameters was assessed. The results revealed that HDC-MVD in the tumor microenvironment of CRCs was significantly increased as compared with the controls. CRC patients with lymph node invasion had a particularly higher density of HDC-MVD than those without. The density of HDC-MVD accounted for ~79 % of CD34 positive MVD in CRCs and double IHC analysis demonstrated that these HDC positive microvessels were mostly CD34 positive microvessels and with a high proliferative activity. Our results suggest that histamine expressed in microvessels could be an additional cellular source and involved in the cancer invasion through promoting angiogenesis in human CRCs.
authors.affiliation: Department of Medicine, the Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Cui
authors.forename: Gang
authors.initials: G
authors.lastname: Xu
authors.forename: Jinzhong
authors.initials: J
authors.lastname: Liu
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Pang
authors.forename: Jon
authors.initials: J
authors.lastname: Florholmen
authors.forename: Guanglin
authors.initials: G
authors.lastname: Cui
mesh_terms: D018952:Antigens, CD34 / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000098:blood supply* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D005260:Female; D006632:Histamine / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D006640:Histidine Decarboxylase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D008207:Lymphatic Metastasis / Q000235:genetics / Q000473:pathology; D008297:Male; D008407:Mast Cells / Q000378:metabolism* / Q000473:pathology; D055806:Microvessels / Q000378:metabolism / Q000473:pathology; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D011379:Prognosis; D059016:Tumor Microenvironment / Q000235:genetics
chemical_list: D018952:Antigens, CD34; D006632:Histamine; D006640:Histidine Decarboxylase