title: [Effect of Fasudil on miroglia and astrocytes in experimental autoimmune encephalomyelitis mice].
abstract: OBJECTIVE
  To explore the therapeutic effect of Fasudil and its possible mechanisms in experimental autoimmune encephalomyelitis (EAE) mice,  mainly focusing on the roles of microglia and astrocytes in the treatment.
  
  
  METHODS
  Female adult C57BL/6 mice were immunized with MOG35-55 to induce chronic EAE. Fasudil was injected on day 3 p.i. (early Fasudil treatment),  or at the onset of EAE (late Fasudil treatment). Normal saline was injected in other mice as EAE controls in a similar manner. Clinical score and body mass  were recorded every other day. The expressions of iNOS on microglia and p-NF-κB/p65 on astrocytes were measured by immunohistochemistry and Western blotting. The levels of IL-1β and TNF-α in spinal cord homogenate were determined by ELISA.
  
  
  RESULTS
  Fasudil delayed onset and ameliorated the severity of EAE. Fasudil inhibited the expression of iNOS on microglia and p-NF-κB/p65 on astrocytes in spinal cords,  accompanied by the inhibition of inflammatory factors IL-1β  and TNF-α.
  
  
  CONCLUSIONS
  Fasudil exhibits therapeutic effect on EAE,  possibly through inhibiting inflammatory molecules on microglia and astrocyte.
authors.affiliation: Institute of Brain Science, Shanxi Datong University, Datong 037009, China.
authors.forename: Yan-hua
authors.initials: YH
authors.lastname: Li
authors.forename: Chun-yun
authors.initials: CY
authors.lastname: Liu
authors.forename: Pei-jun
authors.initials: PJ
authors.lastname: Zhang
authors.forename: Jie-zhong
authors.initials: JZ
authors.lastname: Yu
authors.forename: Ning
authors.initials: N
authors.lastname: Ji
authors.forename: Yan-yan
authors.initials: YY
authors.lastname: Yan
authors.forename: Ling
authors.initials: L
authors.lastname: Feng
authors.forename: Hai-fei
authors.initials: HF
authors.lastname: Zhang
authors.forename: Bao-guo
authors.initials: BG
authors.lastname: Xiao
authors.forename: Cun-gen
authors.initials: CG
authors.lastname: Ma
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000008:administration & dosage / Q000031:analogs & derivatives*; D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000276:immunology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology; D005260:Female; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology; D009457:Neuroglia / Q000187:drug effects* / Q000276:immunology; D013116:Spinal Cord / Q000187:drug effects / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology
chemical_list: D053583:Interleukin-1beta; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C049347:fasudil