title: [Effect of Leonurus stachydrine on myocardial cell hypertrophy].
abstract: OBJECTIVE
  To investigate the effects of Leonurus stachydrine on myocardial cell hypertrophy induced by angiotensin II in rats.
  
  
  METHODS
  Myocardial cells of neonatal rats were incubated with angiotensin II for 48 hours to establish myocardial cell hypertrophy models. The cell surface area and protein/DNA ratio were detected as index for hypertrophy of myocardial cells. ROS positive myocardial cell were counted by means of flow cytometry, the expression of p-IkappaB (ser32) protein in the cytosol and NF-kappaB (p65) protein in the nucleus were also detected by Western blot.
  
  
  RESULTS
  Various concentrations of stachydrine intervention (10(-6) - 10(-4) mol/L) showed better anti-hypertrophic effect and decreased the number of ROS positive myocardial cell (P<0.05). Meanwhile, intervention of stachydrine significantly suppressed the level of p-IkappaB (ser32) protein in the cytosol and NF-kappaB (p65) protein in the nucleus (P<0.05).
  
  
  CONCLUSIONS
  Stachydrine can inhibit the NF-kappaB signal pathway, and this may be ralated to the mechanism of anti-hypertrophic.
authors.affiliation: Shanghai University of TCM, Shanghai 201203, China. guowei311@yahoo.com.cn
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.forename: Yue-Ling
authors.initials: YL
authors.lastname: Liao
authors.forename: Rong
authors.initials: R
authors.lastname: Lv
authors.forename: Hong-Chang
authors.initials: HC
authors.lastname: Wei
mesh_terms: D000804:Angiotensin II / Q000009:adverse effects; D000818:Animals; D002478:Cells, Cultured; D006984:Hypertrophy / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D051550:I-kappa B Kinase / Q000378:metabolism; D031332:Leonurus / Q000737:chemistry*; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D016328:NF-kappa B / Q000378:metabolism; D011392:Proline / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D016328:NF-kappa B; D017382:Reactive Oxygen Species; D000804:Angiotensin II; D011392:Proline; D051550:I-kappa B Kinase; C003342:stachydrine