title: Distinct susceptibility to inoculated melanoma and sensitivity to cancer pain in mouse lines with high and low sensitivity to stress.
abstract: Approximately 30 years ago, we developed 2 mouse lines with enhanced and decreased opioid system activity using bidirectional selection for high (high analgesia [HA] line) and low (low analgesia [LA] line) swim stress-induced analgesia. These mouse lines differ substantially in pain sensitivity, measured as hind paw withdrawal latency in a hot plate test. Moreover, compared with the LA mice, the HA mice exhibited reduced energy expenditure under stress and different depression-like behavior as well as higher sensitivity to mutagens and the high frequency of spontaneous and carcinogen-induced tumors. In the current study, we observed distinct differences in the growth rate of orthotopically implanted melanoma and the onset of cancer pain. Whereas the HA line was prone to tumors and carcinogenesis was rapid in all specimens, the LA mice either did not develop tumors (70%) or developed tumors that often regressed spontaneously (30%). Animals from both lines developed robust thermal hypersensitivity in the tumor-bearing paw compared with animals that were injected with saline. However, we found that hyperalgesia in tumor-bearing mice persists for a much shorter time in the HA than in LA mice. Naltrexone, given subcutaneously, restored hyperalgesia in the HA mice, whereas it was ineffective in the LA mice. The results suggest that activity of the opioid system may influence carcinogenesis and the intensity of cancer pain and indicates that HA and LA mice are good models for such studies.
authors.affiliation: Department of Molecular Cytogenetics, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, Magdalenka, Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Sacharczuk
authors.forename: Agnieszka R
authors.initials: AR
authors.lastname: Ragan
authors.forename: Hanna
authors.initials: H
authors.lastname: Szymanska
authors.forename: Anna
authors.initials: A
authors.lastname: Lesniak
authors.forename: Bogdan
authors.initials: B
authors.lastname: Sadowski
authors.forename: Andrzej W
authors.initials: AW
authors.lastname: Lipkowski
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006930:Hyperalgesia / Q000139:chemically induced / Q000235:genetics* / Q000503:physiopathology; D008297:Male; D008545:Melanoma / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D051379:Mice; D023421:Models, Animal; D009271:Naltrexone / Q000009:adverse effects; D009292:Narcotic Antagonists / Q000009:adverse effects; D018847:Opioid Peptides / Q000235:genetics / Q000378:metabolism; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D013312:Stress, Physiological / Q000502:physiology*; D013550:Swimming / Q000502:physiology
chemical_list: D009292:Narcotic Antagonists; D018847:Opioid Peptides; D009271:Naltrexone