title: ¹H, ¹⁵N, ¹³C assignment and secondary structure determination of two domains of La protein from D. discoideum.
abstract: Biosynthesis of RNA polymerase III transcripts requires binding of the La protein at their 3' end. La is an abundant nuclear RNA-binding protein which protects the nascent transcripts from 3' exonuclease degradation. Here, we report the high yield expression and preliminary structural analysis through NMR spectroscopy of two recombinant RNA binding domains (La motif and NRRM) from the La protein of Dictyostelium discoideum. Both recombinant protein constructs were well-folded and allowed for an almost complete sequence-specific assignment of the (15)N and (13)C labeled domains and their secondary structure prediction using PECAN online tool.
authors.affiliation: Department of Biochemistry, School of Medicine, University of Patras, 26504, Patras, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Apostolidi
authors.forename: Dionysios J
authors.initials: DJ
authors.lastname: Vourtsis
authors.forename: Christos T
authors.initials: CT
authors.lastname: Chasapis
authors.forename: Constantinos
authors.initials: C
authors.lastname: Stathopoulos
authors.forename: Detlef
authors.initials: D
authors.lastname: Bentrop
authors.forename: Georgios A
authors.initials: GA
authors.lastname: Spyroulias
mesh_terms: D020816:Amino Acid Motifs; D002247:Carbon Isotopes; D004023:Dictyostelium / Q000378:metabolism*; D006859:Hydrogen; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000737:chemistry*
chemical_list: D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D015800:Protozoan Proteins; D006859:Hydrogen