title: Gold nanoflower@gelatin core-shell nanoparticles loaded with conjugated polymer applied for cellular imaging.
abstract: In the present work, a facile one-pot method is designed to fabricate a core-shell fluorescent nanoparticle (NP) for cellular imaging based on a new cationic conjugated polymer, poly[9,9'-bis(6,6'-(N,N,N-trimethylaminium)fluorene-2,7-ylenevinylene-co-alt-2,5-dicyano-1,4-phenylene] (PFVCN). Gold nanoflowers (AuNFs) are prepared by a seedless method, in which a gelatin layer formed through a sol-gel phase transition is deposited on the surface of each AuNF. The cationic PFVCN self-assembles onto the negative surface of the resultant (AuNF@Gelatin NPs) driven by electrostatic attraction. An obvious enhancement of fluorescence intensity is observed. The AuNF@Gelatin/PFVCN NPs exhibit excellent cytocompatibility, and their cellular imaging ability is demonstrated when cocultured with HeLa cells. AuNF@Gelatin/PFVCN hybrid NPs are expected to be a desirable material in the field of cellular imaging and biosensing.
authors.affiliation: State Key Laboratory for Advanced Metals and Materials, School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, China.
authors.forename: Qianling
authors.initials: Q
authors.lastname: Cui
authors.forename: Fang
authors.initials: F
authors.lastname: He
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
authors.forename: Bihua
authors.initials: B
authors.lastname: Xia
authors.forename: Lidong
authors.initials: L
authors.lastname: Li
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D005449:Fluorenes / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry* / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D008856:Microscopy, Fluorescence; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D005449:Fluorenes; D011108:Polymers; C041509:fluorene; D006046:Gold; D005780:Gelatin