title: [Effect of drinking boron on microtructure of adrenal gland in rats].
abstract: OBJECTIVE
  The effects of drinking boron exposure on the mass, organ indexes and structure of adrenal gland were studied in the paper. Methods 192 Sprague-Dawley rats (28 +/- 2 days) with no bacteria infecting were divided into six groups (n = 32, male = female) randomly. Treated rats drunk the distilled water which supplemented with boron of 0, 40, 80, 160, 320 and 640 mg/L, respectively, for 60 days. At the 30th and the 60th day of experiment, 16 rats (n = 8, male = female) of each group were selected and made into narcosis with 10% Chloral Hydrate. The adrenal glands were obtained, weighted and fixed after dissection, then the samples were made into paraffin sections, stained with HE stain and chromaffin, observed and photographed by Olympus CH-30 microphotograph system.
  
  
  RESULTS
  Compared with control group, the average mass of adrenal gland of male rats in each experiment group decreased significantly or most significantly at the 30th day of experiment (P < 0.05 or P < 0.01), but the index of adrenal gland of male rats in the group of 640 mg/L boron at 60th day of experiment increased significantly (P < 0.05). Under the microscope, the microstructure of adrenal gland of rats in the group of 40 mg/L boron were better obviously than control group, and the numbers of chromaffin granules in chromaffin cell increased obviously. The histopathological changes of different degree could be observed in the group of 80 to 640 mg/L boron, and they became remarkable with the boron supplementation. By comparative observation, the damage of cells in adrenal medulla appeared ahead of them in adrenal cortex, and the pathological change of adrenal gland in male rats were obvious than female rats.
  
  
  CONCLUSIONS
  Drinking supplemented with 40 mg/L boron could prompt the structure of adrenal gland in rats, but could cause different degree damage, or even obvious toxic effect when the concentration of boron supplementation in drinking from 80 to 640 mg/L.
authors.affiliation: Anhui Science and Technology University, Fengyang 233100, China. lishhe2005@163.com
authors.forename: Shenghe
authors.initials: S
authors.lastname: Li
authors.forename: Jue
authors.initials: J
authors.lastname: Wang
authors.forename: Jinxing
authors.initials: J
authors.lastname: Zhou
authors.forename: Guangming
authors.initials: G
authors.lastname: Jin
authors.forename: Youfang
authors.initials: Y
authors.lastname: Gu
authors.forename: Wanxiang
authors.initials: W
authors.lastname: Xu
mesh_terms: D000311:Adrenal Glands / Q000648:ultrastructure*; D000818:Animals; D001895:Boron / Q000032:analysis / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D060766:Drinking Water / Q000032:analysis*; D052244:Endocrine Disruptors / Q000032:analysis / Q000633:toxicity; D005260:Female; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014873:Water Pollutants / Q000032:analysis / Q000633:toxicity*
chemical_list: D060766:Drinking Water; D052244:Endocrine Disruptors; D014873:Water Pollutants; D001895:Boron