title: Preparation of phenylboronic acid-silica hybrid monolithic column with one-pot approach for capillary liquid chromatography of biomolecules.
abstract: A phenylboronic acid-silica hybrid monolithic column for capillary liquid chromatography (cLC) was prepared through one-pot process by using 4-vinylphenylboronic acid (VPBA) and alkoxysilanes simultaneously. The effects of the molar ratio of tetramethyloxysilane/γ-methacryloxypropyltrimethoxysilane (TMOS/γ-MAPS), amount of VPBA, and the volume of diethylene glycol (DEG) on the morphologies, permeabilities and pore properties of the prepared VPBA-silica hybrid monolithic columns were studied in detail. A relatively uniform monolithic structure with high porosity was obtained with optimized ingredients. A series of cis-diol-containing compounds, alkylbenzenes, amides, and anilines were utilized to evaluate the retention behaviors of the VPBA-silica hybrid monolithic column. The result demonstrated that the prepared VPBA-silica hybrid monolithic column exhibited multiple interactions including hydrophobicity, hydrophilicity, as well as cation exchange apart from the expected affinity interaction. The run-to-run, column-to-column and batch-to-batch reproducibility of the VPBA-silica hybrid monolith were satisfactory with the relative standard deviations (RSDs) less than 1.63% (n=5), 2.02% (n=3) and 2.90% (n=5), respectively, indicating the effectiveness and practicability of the proposed method. In addition, the VPBA-silica hybrid monolithic column was further applied to the separation of proteins and tryptic digest of bovine serum albumin (BSA), respectively. The successful applications suggested the potential of the VPBA-silica hybrid monolith in proteome analysis.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China. zianlin@fzu.edu.cn
authors.forename: Zian
authors.initials: Z
authors.lastname: Lin
authors.forename: Hui
authors.initials: H
authors.lastname: Huang
authors.forename: Shihua
authors.initials: S
authors.lastname: Li
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Tan
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D001555:Benzene Derivatives / Q000737:chemistry; D001897:Boronic Acids / Q000737:chemistry*; D002417:Cattle; D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D014753:Vinyl Compounds / Q000737:chemistry*
chemical_list: C427523:4-vinylphenyl boronic acid; D000097:Acetonitriles; D001555:Benzene Derivatives; D001897:Boronic Acids; D010446:Peptide Fragments; D014753:Vinyl Compounds; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; C032159:acetonitrile