title: Elicitation of galanthamine production by Leucojum aestivum shoots grown in temporary immersion system.
abstract: The influence of different elicitors (copper sulfate, silver nitrate, salicylic acid and methyl jasmonate), on both the growth and alkaloid production of Leucojum aestivum shoots grown in a temporary immersion system was studied. Seven Amaryllidaceae alkaloids and three protoalkaloids were quantitatively determined by GC-MS analysis in leaves and bulblets, separately. Methyl jasmonate was found to significantly improve the production of galanthamine (GAL) in both leaves and bulblets. The content of GAL released to the liquid nutrient medium was also measured. The release of GAL into the liquid medium took place mainly in the first 2 weeks determined by harvesting the liquid nutrient medium after 2 weeks and measuring the GAL content (1st subculturing step).
authors.affiliation: Vita 34 AG, Deutscher Platz 5a, 04103, Leipzig, Germany. anika.schumann@vita34.de
authors.forename: Anika
authors.initials: A
authors.lastname: Schumann
authors.forename: Laura
authors.initials: L
authors.lastname: Torras-Claveria
authors.forename: Strahil
authors.initials: S
authors.lastname: Berkov
authors.forename: Diana
authors.initials: D
authors.lastname: Claus
authors.forename: André
authors.initials: A
authors.lastname: Gerth
authors.forename: Jaume
authors.initials: J
authors.lastname: Bastida
authors.forename: Carles
authors.initials: C
authors.lastname: Codina
mesh_terms: D003470:Culture Media / Q000032:analysis; D046508:Culture Techniques / Q000379:methods*; D005702:Galantamine / Q000096:biosynthesis*; D019668:Liliaceae / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D018520:Plant Shoots / Q000737:chemistry / Q000378:metabolism*
chemical_list: D003470:Culture Media; D005702:Galantamine