title: [Diversity study of arbuscular mycorrhizal fungi of Coptis deltoidea].
abstract: OBJECTIVE
  To investigate the resources and distribution of Arbuscular Mycorrhizal Fungi (AMF) in rhizosphere of Coptis deltoidea.
  
  
  METHODS
  The colonization situation of AMF of Coptis deltoidea were detected by acid fuchsin staining and then calculated the colonization rate. AMF fungal spores were obtained by Wet-screening method and then classified and identified by their morphological characteristics and histochemical staining method.
  
  
  RESULTS
  Coptis deltoidea and AMF could engender the mycorrhiza. Colonization rates were different in different producing areas and ranged from 23. 3% to 34.4%. Thirty species of AMF belonging in 6 genera were found, including 17 species of the Acaulospora, 7 of the Glomus, 2 of the Gigaspora, 1 of the Entrophospora, 1 of the Paraglomus and 2 of the Archaeospora were isolated from rhizosphere of Coptis deltoidea. Acaulospora and Glomus were the dominant genera and the AMF species of genuine producing regions in Hongya county was the most diversiform among the six areas.
  
  
  CONCLUSIONS
  Coptis deltoidea is a kind of nutrition type of mycorrhiza plant. The rich diversity of AMF of Coptis deltoidea in producing areas make the AMF is a biological resource of significant application prospects in enlarging yield and non-pollution production.
authors.affiliation: College of Pharmacy, Chengdu University of TCM, Key Lab of Standardization of Chinese Herbal Medicines of Ministry of Education, Chengdu 610075, China. hengnokia5@yahoo.com.cn
authors.forename: Wen-li
authors.initials: WL
authors.lastname: Huang
authors.forename: Xin-jian
authors.initials: XJ
authors.lastname: Fan
authors.forename: Zhu-yun
authors.initials: ZY
authors.lastname: Yan
authors.forename: Yun-tong
authors.initials: YT
authors.lastname: Ma
authors.forename: Xian-li
authors.initials: XL
authors.lastname: Meng
mesh_terms: D044822:Biodiversity; D015169:Colony Count, Microbial / Q000379:methods; D003247:Conservation of Natural Resources; D039323:Coptis / Q000254:growth & development / Q000382:microbiology*; D017753:Ecosystem; D005658:Fungi / Q000145:classification / Q000302:isolation & purification / Q000502:physiology; D038821:Mycorrhizae / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D018517:Plant Roots / Q000254:growth & development / Q000382:microbiology*; D012988:Soil Microbiology; D013172:Spores, Fungal / Q000145:classification / Q000302:isolation & purification / Q000502:physiology