title: [Variation regularity of chemical constituents contained in Euphorbia ebracteolata before and after roasting with vinegar].
abstract: OBJECTIVE
  To study the variation regularity of chemical constituents contained in Euphorbia ebracteolata after vinegar processing.
  
  
  METHODS
  The colorimetric method was adopted for determining the variation of total lactone content in toxic constituents contained in E. ebracteolata decoction, with Kedde as the coloring reagent. The HPLC method was used for detecting the content variation of jolkinolide B and jolkinolide C, both were active constituents contained in E. ebracteolata decoction, before and after roasting with vinegar, in which Kromasil-ODS column (4.6 mm x 250 mm, 5 microm) was adopted, with the detection wavelength of 290 nm, column temperature at 25 degrees C , gradient elution with acetonitrile and water and the flow rate of 1.0 mL x min(-1).
  
  
  RESULTS
  After roasting with vinegar, the total lactone content in E. ebracteolata was reduced from 0.60 to 0.45 mg x g(-1) , with active constituents, jolkinolide B and jolkinolide C, increased to varying degrees. The established chromatographic fingerprint contained risk information and could reflect the overall variation regularity of chemical constituents after roasting with vinegar.
  
  
  CONCLUSIONS
  The chemical constituents of E. ebracteolata show significantly changes, especially a reduced toxicity, after roasting with vinegar. The increase in its efficacy may be related to the variation of these constituents.
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China.
authors.forename: Guo
authors.initials: G
authors.lastname: Zhuang
authors.forename: Lanyun
authors.initials: L
authors.lastname: Wang
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wenwen
authors.initials: W
authors.lastname: Peng
authors.forename: Junsong
authors.initials: J
authors.lastname: Li
authors.forename: Baochang
authors.initials: B
authors.lastname: Cai
mesh_terms: D019342:Acetic Acid / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D028481:Euphorbia / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D019342:Acetic Acid