title: Anti-herpes virus activities of Achyranthes aspera: an indian ethnomedicine, and its triterpene acid.
abstract: The aim of this study was to evaluate the antiviral potential of methanolic extract (ME) of Achyranthes aspera, an Indian folk medicine and one of its pure compound oleanolic acid (OA) against herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). The ME possessed weak anti-herpes virus activity (EC50 64.4μg/ml for HSV-1 and 72.8μg/ml for HSV-2). While OA exhibited potent antiherpesvirus activity against both HSV-1 (EC50 6.8μg/ml) and HSV-2 (EC50 7.8μg/ml). The time response study revealed that the antiviral activity of ME and OA is highest at 2-6h post infection. The infected and drug-treated peritoneal macrophage at specific time showed increased level of pro-inflammatory cytokines (IL6 and IL12). Further, the PCR of DNA from infected cultures treated with ME and OA, at various time intervals, failed to show amplification at 48-72h, similar to that of HSV infected cells treated with acyclovir, indicating that the ME and OA probably inhibit the early stage of multiplication (post infection of 2-6h). Thus, our study demonstrated that ME and OA have good anti-HSV activity, with SI values of 12, suggesting the potential use of this plant.
authors.affiliation: ICMR Virus Unit, ID & BG Hospital, GB-4, First Floor, 57 Dr Suresh C Banerjee Road, Beliaghata, Kolkata 700 010, India.
authors.forename: Hemanta
authors.initials: H
authors.lastname: Mukherjee
authors.forename: Durbadal
authors.initials: D
authors.lastname: Ojha
authors.forename: Paromita
authors.initials: P
authors.lastname: Bag
authors.forename: Himanshu Singh
authors.initials: HS
authors.lastname: Chandel
authors.forename: Souvik
authors.initials: S
authors.lastname: Bhattacharyya
authors.forename: Tapan K
authors.initials: TK
authors.lastname: Chatterjee
authors.forename: Pulok K
authors.initials: PK
authors.lastname: Mukherjee
authors.forename: Sekhar
authors.initials: S
authors.lastname: Chakraborti
authors.forename: Debprasad
authors.initials: D
authors.lastname: Chattopadhyay
mesh_terms: D029044:Achyranthes / Q000737:chemistry*; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D002522:Chlorocebus aethiops; D006561:Herpes Simplex / Q000188:drug therapy / Q000276:immunology / Q000821:virology; D018259:Herpesvirus 1, Human / Q000187:drug effects; D018258:Herpesvirus 2, Human / Q000187:drug effects; D006801:Humans; D007194:India; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000821:virology; D008519:Medicine, Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000494:pharmacology*; D014315:Triterpenes / Q000494:pharmacology*; D014709:Vero Cells
chemical_list: D000998:Antiviral Agents; D010936:Plant Extracts; D014315:Triterpenes