title: Genetic low nephron number hypertension is associated with altered expression of osteopontin and CD44 during nephrogenesis.
abstract: OBJECTIVE
  The study set out to investigate whether the osteopontin (OPN)-CD44-integrin-receptor-system is differently regulated during nephrogenesis in inborn nephron deficit, a major determinant of human primary hypertension and cardiovascular disease in adult life.
  
  
  METHODS
  We compared a genetic rat model with an inherited nephron deficit, the Munich-Wistar-Froemter rat (MWF), to normotensive Wistar rats during nephrogenesis at day 19 of fetal development (E19) and at postpartal day 7 (D7).
  
  
  RESULTS
  Renal OPN mRNA (-75%, P<0.05) and protein expression (-38%, P<0.05) were strongly decreased at E19 in MWF compared to Wistar. Renal mRNA-expression of CD44 was increased at E19 in MWF (+271%, P<0.05). At D7, renal OPN protein expression was increased (+115%, P<0.05) and renal mRNA-expression of CD44 remained elevated compared to Wistar control (+127%, P<0.05).
  
  
  CONCLUSIONS
  Altered fetal expression of the OPN-CD44-integrin-receptor-system in the MWF model points to a possible role in low nephron number hypertension and cardiovascular disease.
authors.forename: Anne
authors.initials: A
authors.lastname: Freese
authors.forename: Markus
authors.initials: M
authors.lastname: Wehland
authors.forename: Florian
authors.initials: F
authors.lastname: Freese
authors.forename: Christian
authors.initials: C
authors.lastname: Bamberg
authors.forename: Reinhold
authors.initials: R
authors.lastname: Kreutz
authors.forename: Lars
authors.initials: L
authors.lastname: Rothermund
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D047109:Fetal Development; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics*; D006977:Hypertension, Renal / Q000235:genetics* / Q000503:physiopathology*; D039564:Integrin alphaV / Q000235:genetics; D009399:Nephrons / Q000002:abnormalities* / Q000254:growth & development / Q000503:physiopathology; D053495:Osteopontin / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011922:Rats, Mutant Strains; D017208:Rats, Wistar
chemical_list: D018960:Hyaluronan Receptors; D039564:Integrin alphaV; D012333:RNA, Messenger; C506897:Spp1 protein, rat; D053495:Osteopontin