title: [Determination of oleanolic acid and ursolic acid in Damnacanthus indicus from different places by RP-hPLC].
abstract: OBJECTIVE
  To establish A RP-HPLC method for determination of oleanolic acid and ursolic acid in Damnacanthus indicus from different places.
  
  
  METHODS
  After ultrasonic extraction with 95% ethanol,the separation was achieved on a Kromasil C18 column at 25 degrees C using methanol-2.0% phosphoric acid solution (90:10, v/v) as the mobile phase at a flow rate of 0.8 mL/min. The detection wavelength was 210 nm.
  
  
  RESULTS
  Oleanolic acid showed good linear at the range of 0.124-1.24 microg (r = 0.9997). The recovery was 97.6% and RSD was 2.2%. Ursolic acid showed good linear at the range of 0.192-1.92 microg (r = 0.9999). The recovery was 102.4% and RSD was 1.9%.
  
  
  CONCLUSIONS
  The proposed method is simple and accurate. It can provide reliable evidence for quality control, development and utilization of Damnacanthus indicus.
authors.affiliation: Department of Pharmacy, Guangzhou Medical University Cancer Institute Hospital, Guangzhou 510095, China. 553852698@qq.com
authors.forename: Ye-fen
authors.initials: YF
authors.lastname: Cai
authors.forename: Qing-song
authors.initials: QS
authors.lastname: Huang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D009828:Oleanolic Acid / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development; D011786:Quality Control; D015203:Reproducibility of Results; D019666:Rubiaceae / Q000737:chemistry* / Q000254:growth & development; D014315:Triterpenes / Q000032:analysis*; D000097245:Ursolic Acid
chemical_list: D004365:Drugs, Chinese Herbal; D009828:Oleanolic Acid; D014315:Triterpenes; D000097245:Ursolic Acid