title: Comparison of photo- and Cu(I)-catalyzed "click" chemistries for the formation of carbohydrate SPR interfaces.
abstract: Understanding interactions of glycans with proteins in key biological events has seen the development of various analytical methods such as microarray techniques. Label-free approaches, such as surface plasmon resonance (SPR) techniques are particularly attractive and we explore here the potential of a novel interface composed of lamellar Ti/Au/silicon dioxide derivatized with sugars to probe lectin-sugar interactions by SPR. Two parallel surface functionalization strategies have been developed: one in which azide-functionalized surfaces are linked via a Cu(I) "click" method to alkynyl-derivatized glycan partners and another wherein perfluorophenyl azide-functionalized surfaces are reacted through a C-H insertion photocoupling reaction with underivatized glycans. The effectiveness of the two interfaces is assessed for their lectin-recognition abilities in an SPR format.
authors.affiliation: Institut de Recherche Interdisciplinaire (IRI, USR CNRS 3078), Université Lille 1, Parc de la Haute Borne, 50 Avenue de Halley, BP 70478, 59658 Villeneuve d'Ascq, France.
authors.forename: Nazek
authors.initials: N
authors.lastname: Maalouli
authors.forename: Alexandre
authors.initials: A
authors.lastname: Barras
authors.forename: Aloysius
authors.initials: A
authors.lastname: Siriwardena
authors.forename: Mohamed
authors.initials: M
authors.lastname: Bouazaoui
authors.forename: Rabah
authors.initials: R
authors.lastname: Boukherroub
authors.forename: Sabine
authors.initials: S
authors.lastname: Szunerits
mesh_terms: D001565:Benzoates / Q000737:chemistry; D002384:Catalysis; D057930:Click Chemistry; D003300:Copper / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D037102:Lectins / Q000737:chemistry / Q000378:metabolism; D027806:Lens Plant / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D012822:Silicon Dioxide / Q000737:chemistry; D020349:Surface Plasmon Resonance; D014025:Titanium / Q000737:chemistry; D014466:Ultraviolet Rays
chemical_list: D001565:Benzoates; D037102:Lectins; D011134:Polysaccharides; C098120:methyl 4-azido-2,3,5,6-tetrafluorobenzoate; D006046:Gold; D012822:Silicon Dioxide; D003300:Copper; D014025:Titanium