title: Accelerated determination of selenomethionine in selenized yeast: validation of analytical method.
abstract: The purpose of this study was to reduce the extraction time, to hours instead of days, for quantification of the selenomethionine (SeMet) content of selenized yeast. An accelerated method using microwave-assisted enzymatic extraction and ultrasonication was optimized and applied to certified reference material (selenized yeast reference material (SELM)-1). Quantitation of SeMet in the extracts was performed by liquid chromatography with inductively coupled plasma mass spectrometry. The limits of detection and quantitation were 5 ppb SeMet and 15 ppb SeMet respectively and the signal response was linear up to 1,500 ppb SeMet. The average recovery of spiked SeMet from the selenized yeast matrix was 97.7 %. Analysis of an SELM-1 using this method resulted in 100.9 % recovery of the certified value (3448 ± 146 ppm SeMet). This method is suitable for fast reliable determination of SeMet in selenized yeast.
authors.affiliation: Alltech Biotechnology Centre, Sarney, Summerhill Road, Dunboyne, County Meath, Ireland. pward@alltech.com
authors.forename: Patrick
authors.initials: P
authors.lastname: Ward
authors.forename: Cathal
authors.initials: C
authors.lastname: Connolly
authors.forename: Richard
authors.initials: R
authors.lastname: Murphy
mesh_terms: D002853:Chromatography, Liquid; D013058:Mass Spectrometry; D008872:Microwaves; D010447:Peptide Hydrolases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000378:metabolism*; D012645:Selenomethionine / Q000032:analysis* / Q000378:metabolism; D013010:Sonication
chemical_list: D012645:Selenomethionine; D010447:Peptide Hydrolases