title: Porcine radial artery decellularization by high hydrostatic pressure.
abstract: Many types of decellularized tissues have been studied and some have been commercially used in clinics. In this study, small-diameter vascular grafts were made using HHP to decellularize porcine radial arteries. One decellularization method, high hydrostatic pressure (HHP), has been used to prepare the decellularized porcine tissues. Low-temperature treatment was effective in preserving collagen and collagen structures in decellularized porcine carotid arteries. The collagen and elastin structures and mechanical properties of HHP-decellularized radial arteries were similar to those of untreated radial arteries. Xenogeneic transplantation (into rats) was performed using HHP-decellularized radial arteries and an untreated porcine radial artery. Two weeks after transplantation into rat carotid arteries, the HHP-decellularized radial arteries were patent and without thrombosis. In addition, the luminal surface of each decellularized artery was covered by recipient endothelial cells and the arterial medium was fully infiltrated with recipient cells.
authors.affiliation: Division of Biofunctional Molecules, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Negishi
authors.affiliation: Division of Biofunctional Molecules, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Japan.|Japan Science and Technology Agency (CREST), Saitama, Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Funamoto
authors.affiliation: Division of Biofunctional Molecules, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Japan.|Japan Science and Technology Agency (CREST), Saitama, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kimura
authors.affiliation: Division of Biofunctional Molecules, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Japan.|Japan Science and Technology Agency (CREST), Saitama, Japan.
authors.forename: Kwangoo
authors.initials: K
authors.lastname: Nam
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Sapporo Medical University School of Medicine, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Higami
authors.affiliation: Division of Biofunctional Molecules, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Japan.|Japan Science and Technology Agency (CREST), Saitama, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Kishida
mesh_terms: D000818:Animals; D001158:Arteries / Q000166:cytology*; D001807:Blood Vessel Prosthesis; D002339:Carotid Arteries / Q000473:pathology; D003094:Collagen / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D042783:Endothelial Cells / Q000166:cytology; D006874:Hydrostatic Pressure; D008297:Male; D011312:Pressure; D017534:Radial Artery / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013537:Sutures; D013552:Swine; D013696:Temperature; D013927:Thrombosis / Q000473:pathology; D023822:Tissue Engineering / Q000379:methods*; D014183:Transplantation, Heterologous
chemical_list: D003094:Collagen; D004247:DNA