title: [Proteomics analysis of the synergy effect of Radix Hedysari polysaccharides combined with chemotherapy on S180 tumor cells].
abstract: OBJECTIVE
  To analyze the differentially expressed proteins of the synergy effect of Radix Hedysari polysaccharides (HPS)combined with chemotherapy (Cy) on S180 tumor cells.
  
  
  METHODS
  The total proteins extracted from the HPS combined with Cy treated S180 cells in tumor-bearing mice were separated by two dimentional gel electrophoresis (2-DE)and compared with those from Cy treated S180 cells using PDQuest 8.0 software. Mass spectrometry was applied to identify the differentially expressed proteins. Western blot was used to determine the differential expression of one protein.
  
  
  RESULTS
  Twenty-four differentially-expressed proteins in HPS group were discovered. The five differential expressed proteins among twenty-four proteins were later identified by mass spectrometry and Mascot software as heat-shock protein hsp84 (HSP90beta), apolipoprotein A, albumin, heat shock protein beta-1 (HSP27)and unnamed protein product, including one up regulated and four down regulated expressed proteins respectively. Results from Western blot manifested the same trend as from proteomics analysis.
  
  
  CONCLUSIONS
  Proteomics technique can be used to discover target proteins associated with the synergy effect of HPS combined with Cy on S180 tumor cells, involving some important proteins related to energy metabolism, oxidative stress and apoptosis induction signal transduction.
authors.affiliation: Institute of Integrated Chinese and Western Medicine, Lanzhou University, Lanzhou 730000, China. weidongfeng888@yahoo.cn
authors.forename: Dong-feng
authors.initials: DF
authors.lastname: Wei
authors.forename: Chun-yan
authors.initials: CY
authors.lastname: Zhao
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Cheng
authors.forename: Yan-xia
authors.initials: YX
authors.lastname: Wei
authors.forename: Li-feng
authors.initials: LF
authors.lastname: Zhang
authors.forename: Gang
authors.initials: G
authors.lastname: Su
mesh_terms: D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D045744:Cell Line, Tumor; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000494:pharmacology*; D004359:Drug Therapy, Combination; D015180:Electrophoresis, Gel, Two-Dimensional; D007887:Fabaceae / Q000737:chemistry*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D008297:Male; D051379:Mice; D011134:Polysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics; D012510:Sarcoma 180 / Q000378:metabolism* / Q000473:pathology
chemical_list: D011134:Polysaccharides; D011506:Proteins; D003520:Cyclophosphamide