title: The synthesis, transportation and degradation of BmLP3 and BmLP7, two highly homologous Bombyx mori 30K proteins.
abstract: The 30K proteins comprise about 35% of the total embryo yolk proteins and function as storage proteins during embryonic development of the domesticated silkworm Bombyx mori. The most abundant components of hemolymph are 30K proteins in the early and middle pupal stages. In the present study, the 30K protein BmLP7 was purified from larval hemolymph by chromatography. We prepared the antibody of this protein and found that it could bind to both BmLP3 and BmLP7. We used western blotting to analyze the dynamic change of BmLP3 and BmLP7 proteins in the hemolymph during development and found their concentration decreased dramatically from day 4 pupae, which appears to be linked to their accumulation in the oocyte for forming yolk granule since then. We found BmLP3 and BmLP7 proteins reduced significantly in day 10 eggs (the day before hatching). The crude extract of the newly hatched larvae showed proteolytic activity against BmLP3 and BmLP7 and immunohistochemistry showed BmLP3 and BmLP7 were degraded in the embryonic gut lumen in day 10 eggs. These systematic studies of BmLP3 and BmLP7 reveal their synthesis, transportation and degradation, which could represent the experience of all 30K proteins.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Zhao
authors.forename: Hongli
authors.initials: H
authors.lastname: Liu
authors.forename: Zhaoming
authors.initials: Z
authors.lastname: Dong
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yang
authors.forename: Dandan
authors.initials: D
authors.lastname: Wang
authors.forename: Qingyou
authors.initials: Q
authors.lastname: Xia
mesh_terms: D000818:Animals; D012831:Bombyx / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D047108:Embryonic Development; D005216:Fat Body / Q000378:metabolism*; D005260:Female; D006458:Hemolymph / Q000378:metabolism; D019476:Insect Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D008297:Male; D010053:Ovary / Q000378:metabolism; D010063:Ovum / Q000378:metabolism; D011817:Rabbits
chemical_list: D019476:Insect Proteins