title: Effect of tricainemethanesulfonate (MS222), clove oil and electro-anaesthesia on respiratory burst activity in whole blood and serum alternative complement response in rainbow trout (Oncorhynchus mykiss), during the narcosis stage.
abstract: There is a little available information on the suppressive effect of anaesthesia on immune response in fish, especially electro-anaesthesia. In the present study, two anaesthetics, MS222 (50 ppm), clove oil (25 ppm), and electro-anaesthesia were tested in rainbow trout (Oncorhynchus mykiss) during the narcosis stage in order to observe their effects on the innate immune system. The results showed that electro-anaesthesia reduces light emission in chemiluminescence assay both 1 and 24 h post anaesthesia. Clove oil and MS222 decreased light emission 24 h post anaesthesia. In addition, clove oil, MS222 and electro-anaesthesia had no effect on alternative complement (ACH50) response. From the perspective of aquaculture practice, these data show that the type of anaesthesia should be taken into account to avoid possible immunosuppression in rainbow trout.
authors.affiliation: Department of Natural Resources, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Golestan, Iran.
authors.forename: H Gholipour
authors.initials: HG
authors.lastname: Kanani
authors.forename: M
authors.initials: M
authors.lastname: Soltani
authors.forename: S S
authors.initials: SS
authors.lastname: Mirzargar
mesh_terms: D062365:Aminobenzoates / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D017756:Aquaculture / Q000379:methods; D045783:Clove Oil / Q000494:pharmacology*; D003170:Complement Pathway, Alternative / Q000187:drug effects; D004580:Electronarcosis / Q000379:methods*; D007113:Immunity, Innate / Q000187:drug effects*; D008163:Luminescent Measurements / Q000662:veterinary; D017686:Oncorhynchus mykiss / Q000276:immunology / Q000378:metabolism*; D016897:Respiratory Burst / Q000187:drug effects; D053608:Stupor / Q000097:blood / Q000139:chemically induced / Q000378:metabolism*
chemical_list: D062365:Aminobenzoates; D045783:Clove Oil; C003636:tricaine