title: Ubiquitin C-terminal Hydrolase 37, a novel predictor for hepatocellular carcinoma recurrence, promotes cell migration and invasion via interacting and deubiquitinating PRP19.
abstract: Ubiquitin C-terminal hydrolase 37 (UCH37) plays a crucial role in numerous biological processes and is also involved in oncogenesis. In this study, clinicopathologic data showed that UCH37 was over-expressed in hepatocellular carcinoma (HCC) cancerous tissues and was a significant predictor for time to recurrence (TTR). In vitro, we discovered that UCH37 could promote cell migration and invasion. Subsequently, we utilized Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) to identify differentially expressed proteins in UCH37 over-expressing cells compared with the control cells, and found that PRP19, an essential RNA splicing factor, was up-regulated. The relationship between UCH37, PRP19 and the capability of cell migration and invasion was further confirmed. Collectively, this study demonstrated that UCH37 could promote cell migration and invasion in HCC cell lines through interacting and deubiquitinating PRP19, and suggested that UCH37 could be a novel predictor for HCC recurrence after curative resection.
authors.affiliation: The Department of Gastroenterology of Zhongshan Hospital, Fudan University, Shanghai, 200032, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Fang
authors.forename: Da
authors.initials: D
authors.lastname: Fu
authors.forename: Wenqing
authors.initials: W
authors.lastname: Tang
authors.forename: Yu
authors.initials: Y
authors.lastname: Cai
authors.forename: Duan
authors.initials: D
authors.lastname: Ma
authors.forename: Huijun
authors.initials: H
authors.lastname: Wang
authors.forename: Ruyi
authors.initials: R
authors.lastname: Xue
authors.forename: Taotao
authors.initials: T
authors.lastname: Liu
authors.forename: Xiaowu
authors.initials: X
authors.lastname: Huang
authors.forename: Ling
authors.initials: L
authors.lastname: Dong
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Xizhong
authors.initials: X
authors.lastname: Shen
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D002268:Carboxypeptidases / Q000235:genetics / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000401:mortality / Q000556:secondary; D002448:Cell Adhesion; D002453:Cell Cycle; D002465:Cell Movement; D049109:Cell Proliferation; D045643:DNA Repair Enzymes / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D047468:Immunoprecipitation; D008113:Liver Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009364:Neoplasm Recurrence, Local / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D011499:Protein Processing, Post-Translational; D000072260:RNA Splicing Factors; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015996:Survival Rate; D025801:Ubiquitin / Q000378:metabolism*; D043222:Ubiquitin Thiolesterase; D014945:Wound Healing
chemical_list: D009687:Nuclear Proteins; D000072260:RNA Splicing Factors; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D025801:Ubiquitin; D002268:Carboxypeptidases; C421245:UCHL5 protein, human; D043222:Ubiquitin Thiolesterase; D045643:DNA Repair Enzymes; C417041:PRPF19 protein, human