title: Enhancement of 2-keto-gulonic acid yield by serial subcultivation of co-cultures of Bacillus cereus and Ketogulonicigenium vulgare.
abstract: The co-cultures of Ketogulonicigenium vulgare and Bacillus cereus were subcultured daily for a total of 150 transfers. The yield of 2-keto-gulonic acid (2-KGA) and medium pH in the co-cultures were measured. We found that the serial subcultivation increased the yield of 2-KGA from 77% (original co-culture) to 93% (the 150th transfer of transferred co-culture). The resulted strains are of industrial interests for vitamin C production.
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, PO Box 6888, Tianjin 300072, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zou
authors.forename: Menglong
authors.initials: M
authors.lastname: Hu
authors.forename: Yajin
authors.initials: Y
authors.lastname: Lv
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Song
authors.forename: Ying-Jin
authors.initials: YJ
authors.lastname: Yuan
mesh_terms: D001205:Ascorbic Acid / Q000096:biosynthesis; D001409:Bacillus cereus / Q000254:growth & development / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D018929:Cell Culture Techniques / Q000379:methods*; D005285:Fermentation; D041902:Rhodobacteraceae / Q000254:growth & development / Q000378:metabolism*; D013013:Sorbose / Q000032:analysis; D013400:Sugar Acids / Q000032:analysis / Q000378:metabolism*
chemical_list: D013400:Sugar Acids; C015252:gulonic acid; D013013:Sorbose; D001205:Ascorbic Acid