title: Comparative dynamic distribution of avian infectious bronchitis virus M41, H120, and SAIBK strains by quantitative real-time RT-PCR in SPF chickens.
abstract: Avian infectious bronchitis is an acute, highly contagious disease of chickens. To study the differences of dynamic distribution between nephropathogenic infectious bronchitis virus (IBV) strains such as SAIBK and other strains (the M41 and H120 strains), relative quantitative real-time reverse transcription-polymerase chain reaction was developed by housekeeping gene selection. Glyceraldehyde-3-phosphate dehydrogenase and Ubiquitin were chosen for normalization in this experimental set. Then nine tissues, the trachea, thymus, liver, spleen, lungs, kidney, pancreas, proventriculus, and bursa of Fabricius, were analyzed and compared to determine the tropism of IBV infection. In this research, the kidney and the lung were established of the most sensitive organs in IBV infection. The pancreas and the liver are candidates for antigen detection. The trachea and the spleen can be used as references for histological diagnosis, but they are not suitable for antigen detection; proventriculus might be an important target in IBV infection; the thymus and the bursa of Fabricius were not sensitive organs in IBV infection.
authors.affiliation: School of Life Sciences, Sichuan University, Animal Disease Prevention and Food Safety, Key Laboratory of Sichuan Province, Ministry of Education, 985 Project Science Innovative Platform for Resource and Environment Protection of Southwestern China, Chengdu, China.
authors.forename: Wen-qiao
authors.initials: WQ
authors.lastname: Fan
authors.forename: Hong-ning
authors.initials: HN
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhong-bin
authors.initials: ZB
authors.lastname: Guan
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.forename: Chang-wen
authors.initials: CW
authors.lastname: Xu
authors.forename: An-yun
authors.initials: AY
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
mesh_terms: D000818:Animals; D002645:Chickens / Q000821:virology*; D020043:Genes, Essential / Q000235:genetics; D001351:Infectious bronchitis virus / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity; D013047:Specific Pathogen-Free Organisms