title: Evaluation of an Experimental Gel Containing Euclea natalensis: An In Vitro Study.
abstract: Objective. To evaluate the effect of an experimental gel containing Euclea natalensis extract on dentin permeability. Methods. Thirty-six dentin discs, 1-mm-thick. The discs were prepared from the coronal dentin of extracted human third molars that were divided into 3 groups (n = 10). The dentin discs in each group were treated with the groups following experimental materials: (FG): 1.23% fluoride gel, pH 4.1; (EG): Euclea natalensis extract gel, pH 4.1; (CG): control gel, pH 4.1. The gels were applied to the occlusal slide of the dentin under the following conditions: after 37% phosphoric acid and before 6% citric acid. The hydraulic conductance (HC) of each condition was determined four times using a fluid flow apparatus (Flodec). The data were analyzed using Two-way ANOVA and Tukey's test (P < 0.05). Results. The greatest mean reduction in HC was produced in group EG dentin discs (61.2%; P < 0.05). Even after acid challenge with 6% citric acid the great reduction occurred in group EG (66.0%; P < 0.05) than other groups (CG-77.1%, FG-90.8%). Conclusion. E. natalensis gel not only reduced dentin permeability, but also resisted posttreatment citric acid challenge without changing its permeability. Further research has to confirm this promising result in the clinical situation.
authors.affiliation: Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisolla, 17012-901 Bauru, SP, Brazil.
authors.forename: Sílvia Helena de Carvalho
authors.initials: SH
authors.lastname: Sales-Peres
authors.forename: Letícia Ferreira de Freitas
authors.initials: LF
authors.lastname: Brianezzi
authors.forename: Juliane Avansini
authors.initials: JA
authors.lastname: Marsicano
authors.forename: Moacir Rossi
authors.initials: MR
authors.lastname: Forim
authors.forename: Maria Fatima das Graças Fernandes
authors.initials: MF
authors.lastname: da Silva
authors.forename: Arsenio
authors.initials: A
authors.lastname: Sales-Peres