title: Short-term effect of cryotherapy on human scleral tissue by atomic force microscopy.
abstract: This study investigated the inflammatory effect of cryotherapy application on collagen matrix network in human infant sclera. Donor scleral tissues taken from three infant patients divided into five groups: control group, sham-treated group, and three cryotreated groups. In the cryotherapy groups, the sclera was treated for 5 s, 10 s, and 20 s with -80°C freezing by a cryosurgical system. The cryotreated reactions were examined using double histological analysis with hematoxylin-eosin and Masson's trichrome, and atomic force microscopy analysis to quantify the diameter and D-banding of collagen fibrils. The infant scleral tissues treated with cryotherapy showed a significantly increased collagen density associated with inflammatory response (p < 0.05), increased fibril diameter (p < 0.005) compared to the scleral tissues in the control group. The results directly suggest that the cryotherapy affects the morphology of scleral collagen.
authors.affiliation: Department of Ophthalmology, Kyung Hee University, Seoul, Korea.
authors.forename: Seung Jun
authors.initials: SJ
authors.lastname: Lee
authors.forename: Samjin
authors.initials: S
authors.lastname: Choi
authors.forename: Moo Sang
authors.initials: MS
authors.lastname: Kim
authors.forename: Youjin
authors.initials: Y
authors.lastname: Cheong
authors.forename: Hyung-Woo
authors.initials: HW
authors.lastname: Kwak
authors.forename: Hun-Kuk
authors.initials: HK
authors.lastname: Park
authors.forename: Kyung-Hyun
authors.initials: KH
authors.lastname: Jin
mesh_terms: D003094:Collagen / Q000032:analysis; D017679:Cryotherapy / Q000009:adverse effects*; D006651:Histocytochemistry; D006801:Humans; D007223:Infant; D007249:Inflammation; D018625:Microscopy, Atomic Force; D012590:Sclera / Q000648:ultrastructure*; D015422:Scleral Diseases / Q000628:therapy*
chemical_list: D003094:Collagen