title: Mutagenic/recombinogenic effects of four lipid peroxidation products in Drosophila.
abstract: The human diet is an important factor in the development of different diseases. Lipid peroxidation during frying in edible vegetable liquid oils of food components is a mechanism leading to the formation of free radicals. Such radicals induce tissue damage and are implicated in diverse pathological conditions, including aging, atherosclerosis, brain disorders, cancer, lung disorders and various liver disorders. In the present study, we decided to investigate the genotoxic effects of four lipid peroxidation products in the in vivo Drosophila wing somatic mutation and recombination test. In this test, point mutation, chromosome breakage and mitotic recombination produce single spots; while twin spots are produced only by mitotic recombination. Drosophila is a suitable eukaryotic organism for mutagenicity studies and also its metabolism is quite similar to that of mammalians. Since conflicting data exist on the possible risk of several lipid peroxidation products for humans, we have selected four of them, namely acrolein, crotonaldehyde, 4-hydroxy-hexenal (4-HHE) and 4-oxo-2-nonenal (4-ONE). Especially at the highest concentrations tested all exert both mutagenic and recombinogenic effects in the Drosophila SMART assay, showing a direct dose-effect relationship. This is the first study reporting genotoxicity data in Drosophila for these compounds.
authors.affiliation: Akdeniz University, Faculty of Sciences, Department of Biology, 07058 Campus, Antalya, Turkey. esrefdemir@akdeniz.edu.tr
authors.forename: Eşref
authors.initials: E
authors.lastname: Demir
authors.forename: Fatma
authors.initials: F
authors.lastname: Turna
authors.forename: Bülent
authors.initials: B
authors.lastname: Kaya
authors.forename: Amadeu
authors.initials: A
authors.lastname: Creus
authors.forename: Ricard
authors.initials: R
authors.lastname: Marcos
mesh_terms: D000171:Acrolein / Q000009:adverse effects / Q000032:analysis; D000447:Aldehydes / Q000009:adverse effects / Q000032:analysis; D000818:Animals; D019457:Chromosome Breakage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics*; D015227:Lipid Peroxidation / Q000502:physiology*; D008938:Mitosis; D009152:Mutagenicity Tests; D009153:Mutagens / Q000009:adverse effects*; D009154:Mutation; D011995:Recombination, Genetic / Q000235:genetics*; D014921:Wings, Animal / Q000187:drug effects
chemical_list: C403894:4-oxo-2-nonenal; D000447:Aldehydes; D009153:Mutagens; C063409:4-hydroxyhexenal; D000171:Acrolein; C012796:2-butenal