title: Elastic three-dimensional poly (ε-caprolactone) nanofibre scaffold enhances migration, proliferation and osteogenic differentiation of mesenchymal stem cells.
abstract: OBJECTIVE
  We prepared 3D poly (ε-caprolactone) (PCL) nanofibre scaffolds and tested their use for seeding, proliferation, differentiation and migration of mesenchymal stem cell (MSCs).
  
  
  METHODS
  3D nanofibres were prepared using a special collector for common electrospinning; simultaneously, a 2D PCL nanofibre layer was prepared using a classic plain collector. Both scaffolds were seeded with MSCs and biologically tested. MSC adhesion, migration, proliferation and osteogenic differentiation were investigated.
  
  
  RESULTS
  The 3D PCL scaffold was characterized by having better biomechanical properties, namely greater elasticity and resistance against stress and strain, thus this scaffold will be able to find broad applications in tissue engineering. Clearly, while nanofibre layers of the 2D scaffold prevented MSCs from migrating through the conformation, cells infiltrated freely through the 3D scaffold. MSC adhesion to the 3D nanofibre PCL layer was also statistically more common than to the 2D scaffold (P < 0.05), and proliferation and viability of MSCs 2 or 3 weeks post-seeding, were also greater on the 3D scaffold. In addition, the 3D PCL scaffold was also characterized by displaying enhanced MSC osteogenic differentiation.
  
  
  CONCLUSIONS
  We draw the conclusion that all positive effects observed using the 3D PCL nanofibre scaffold are related to the larger fibre surface area available to the cells. Thus, the proposed 3D structure of the nanofibre layer will find a wide array of applications in tissue engineering and regenerative medicine.
authors.affiliation: Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Science of the Czech Republic, Videnska 1083, 142 40, Prague, Czech Republic. m.rampichova@biomed.cas.cz
authors.forename: M
authors.initials: M
authors.lastname: Rampichová
authors.forename: J
authors.initials: J
authors.lastname: Chvojka
authors.forename: M
authors.initials: M
authors.lastname: Buzgo
authors.forename: E
authors.initials: E
authors.lastname: Prosecká
authors.forename: P
authors.initials: P
authors.lastname: Mikeš
authors.forename: L
authors.initials: L
authors.lastname: Vysloužilová
authors.forename: D
authors.initials: D
authors.lastname: Tvrdík
authors.forename: P
authors.initials: P
authors.lastname: Kochová
authors.forename: T
authors.initials: T
authors.lastname: Gregor
authors.forename: D
authors.initials: D
authors.lastname: Lukáš
authors.forename: E
authors.initials: E
authors.lastname: Amler
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D004548:Elasticity; D006801:Humans; D058574:Integrin-Binding Sialoprotein / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis; D011091:Polyesters / Q000737:chemistry*; D044968:Regenerative Medicine; D013499:Surface Properties; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D058574:Integrin-Binding Sialoprotein; D011091:Polyesters; D015675:Osteocalcin; C016240:polycaprolactone