title: Temperature-driven self-actuated microchamber sealing system for highly integrated microfluidic devices.
abstract: We present here a novel microchamber sealing valve that is self-actuated by a pressure change during the temperature change in the thermal activation of reactions. Actuation of our valve requires only the use of the same heating device as employed for the reactions. A thermoplastic UV-curable polymer is used as a device material; the polymer allows realization of the temperature-driven valve actuation as well as the fabrication of multi-layered devices. The self-actuated valve achieves effective sealing of the microchamber for the polymerase chain reaction (PCR) even at 90 °C, which is essential for developing highly parallel PCR array devices without the need for complicated peripherals to control the valve operation.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, FIRST Chikusa-ku, Nagoya, Japan. naito.toyohiro@f.mbox.nagoya-u.ac.jp
authors.forename: Toyohiro
authors.initials: T
authors.lastname: Naito
authors.forename: Rerngchai
authors.initials: R
authors.lastname: Arayanarakool
authors.forename: Séverine
authors.initials: S
authors.lastname: Le Gac
authors.forename: Takao
authors.initials: T
authors.lastname: Yasui
authors.forename: Noritada
authors.initials: N
authors.lastname: Kaji
authors.forename: Manabu
authors.initials: M
authors.lastname: Tokeshi
authors.forename: Albert
authors.initials: A
authors.lastname: van den Berg
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba