title: Multiple steps of HLA-G in ovarian carcinoma metastasis: alter NK cytotoxicity and induce matrix metalloproteinase-15 (MMP-15) expression.
abstract: Previous study showed that aberrant HLA-G expression in cancer cells plays important roles in disease progression and it was associated with tumor metastasis and with poor survival in an animal model with ovarian cancer; however, the mechanisms remain to be explored. In this study, we found that HLA-G expression could dramatically decreased the NK cytotoxicity against the ovarian carcinoma cell line (HO-8910) engineered to express HLA-G (HO-8910-G), and matrix metalloproteinase-15 (MMP-15) expression was up-regulated in HO-8910-G cells. Consistent with this, a strong correlation between HLA-G and MMP-15 expression were observed in a cohort of ovarian cancer samples. Knockdown the HLA-G induced MMP-15 expression by small interfere RNA (siRNA) significantly decreased the HO-8910-G cell migration potential and tumor metastasis. Collectively, our study indicated that HLA-G involved in tumor invasiveness or metastasis may rely on the NK cytotoxicity inhibition and induction of MMP-15 expression in ovarian cancer.
authors.affiliation: Medical Research Center, Taizhou Hospital of Zhejiang Province, Linhai, Zhejiang 317000, PR China.
authors.forename: Aifen
authors.initials: A
authors.lastname: Lin
authors.forename: Hui-Hui
authors.initials: HH
authors.lastname: Xu
authors.forename: Dan-Ping
authors.initials: DP
authors.lastname: Xu
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Yan
mesh_terms: D000818:Animals; D002277:Carcinoma / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D003602:Cytotoxicity, Immunologic; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000276:immunology*; D059951:HLA-G Antigens / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology* / Q000473:pathology; D053517:Matrix Metalloproteinase 15 / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D034741:RNA, Small Interfering
chemical_list: D059951:HLA-G Antigens; D034741:RNA, Small Interfering; D053517:Matrix Metalloproteinase 15