title: FT-IR and NMR study of seed coat dissected from different colored progenies of Brassica napus-Sinapis alba hybrids.
abstract: BACKGROUND
  Yellow-seeded Brassica napus is preferred by breeders for economic reasons, and seed coat is the most obvious comparable character between yellow and black rapeseed.
  
  
  RESULTS
  Owing to an incomplete understanding of chemical components in yellow rapeseed coat, here we optimized a technique based on attenuated total reflectance-Fourier transform infrared (FT-IR) microspectroscopy to screen differences in chemical compositions of Brassica napus, Sinapis alba and four progeny lines with different yellow seed color derived from somatic hybrids between B. napus and S. alba. Nuclear magnetic resonance (NMR) analysis of seed coat from B. napus and S. alba represented two specific peaks representing components of lignin in B. napus, which were absent in the progenies. Also, the intensity of peaks related to fiber were stronger in B. napus than S. alba and yellow seed lines, indicating the differences of fiber and lignin contents in yellow-seeded lines and parent lines. Differences in the lipid, protein and carbohydrate features were identified between B. napus and S. alba, some of which were specifically inherited in progenies of the somatic hybrids.
  
  
  CONCLUSIONS
  FT-IR spectrometry and NMR provide a rapid, direct bioanalytical technique for exploring the changes in molecular chemistry within the canola seed coat and for selecting rapeseed materials with high quality, which can be further used for breeding or nutrition of human and animals.
authors.affiliation: Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Jinjin
authors.initials: J
authors.lastname: Jiang
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Shao
authors.forename: Aimin
authors.initials: A
authors.lastname: Li
authors.forename: Yongtai
authors.initials: Y
authors.lastname: Zhang
authors.forename: Cunxu
authors.initials: C
authors.lastname: Wei
authors.forename: Youping
authors.initials: Y
authors.lastname: Wang
mesh_terms: D029688:Brassica napus / Q000235:genetics*; D003433:Crosses, Genetic; D008055:Lipids; D009682:Magnetic Resonance Spectroscopy; D010860:Pigments, Biological; D012639:Seeds / Q000737:chemistry* / Q000378:metabolism; D031226:Sinapis / Q000235:genetics*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D008055:Lipids; D010860:Pigments, Biological