title: IL-27 increases BST-2 expression in human monocytes and T cells independently of type I IFN.
abstract: IL-27 modulates inflammatory responses by influencing cytokine secretion and CD4 T cell differentiation. Recently, IL-27 was demonstrated to inhibit HIV replication by inducing type I interferon (IFN) expression and subsequent IFN-dependent expression of apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like (APOBEC)-3 family members, a group of antiviral cytidine deaminases. To characterize other anti-viral genes modulated by IL-27, we examined another IFN-responsive gene: tetherin/bone marrow stromal cell antigen 2 (BST-2). Our study shows that IL-27 can directly induce BST-2 expression, independently of an intermediary type I IFN response. Quantitative RT-PCR analysis demonstrated IL-27-induced BST-2 mRNA expression as early as 2h after exposure of cells to IL-27. In the presence of the type I IFN-neutralizing protein, B18R, IL-27-induced BST-2 expression was maintained, demonstrating that IFN is not an intermediary in IL-27-induced BST-2. Taken together, our findings identify a novel function of IL-27 as a direct stimulator of BST-2 expression.
authors.affiliation: Department of Biomedical and Molecular Sciences, Queen's University, Kingston ON, Canada, K7L 3N6.
authors.forename: Christina
authors.initials: C
authors.lastname: Guzzo
authors.forename: Masany
authors.initials: M
authors.lastname: Jung
authors.forename: Ashley
authors.initials: A
authors.lastname: Graveline
authors.forename: Bruce W
authors.initials: BW
authors.lastname: Banfield
authors.forename: Katrina
authors.initials: K
authors.lastname: Gee
mesh_terms: D000071478:APOBEC Deaminases; D015703:Antigens, CD / Q000235:genetics*; D002460:Cell Line; D003564:Cytidine Deaminase; D043525:Cytosine Deaminase / Q000378:metabolism*; D005434:Flow Cytometry; D058851:GPI-Linked Proteins / Q000235:genetics; D006801:Humans; D007370:Interferon Type I / Q000378:metabolism*; D020381:Interleukin-17 / Q000502:physiology*; D009000:Monocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000378:metabolism*; D000074010:Bone Marrow Stromal Antigen 2
chemical_list: D000071478:APOBEC Deaminases; D015703:Antigens, CD; D003564:Cytidine Deaminase; D043525:Cytosine Deaminase; D058851:GPI-Linked Proteins; D007370:Interferon Type I; D020381:Interleukin-17; D012333:RNA, Messenger; C094572:BST2 protein, human; D000074010:Bone Marrow Stromal Antigen 2; C502889:APOBEC3 proteins, human