title: A kinetic method for expeditious detection of pyrophosphate anions at nanomolar concentrations based on a nucleic acid fluorescent sensor.
abstract: A kinetic method has been developed for highly sensitive and selective detection of pyrophosphate anions (PPi) by using a fluorophore labelled single-stranded-DNA-Al(III) complex system. The facile and spectrum-tunable fluorescent sensor enables rapid detection of PPi in urine and cell lysate solutions without the need for complex pre-cleanup procedures.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Su
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.forename: Xianjin
authors.initials: X
authors.lastname: Xiao
authors.forename: Anqin
authors.initials: A
authors.lastname: Xu
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Xu
authors.forename: Meiping
authors.initials: M
authors.lastname: Zhao
mesh_terms: D000535:Aluminum / Q000737:chemistry; D000838:Anions / Q000032:analysis* / Q000652:urine; D002460:Cell Line; D056831:Coordination Complexes / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry*; D011756:Diphosphates / Q000032:analysis* / Q000652:urine; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D000838:Anions; D056831:Coordination Complexes; D004277:DNA, Single-Stranded; D011756:Diphosphates; D005456:Fluorescent Dyes; C107241:diphosphoric acid; D000535:Aluminum