title: 3,5,4'-Tri-O-acetylresveratrol attenuates seawater aspiration-induced lung injury by inhibiting activation of nuclear factor-kappa B and hypoxia-inducible factor-1α.
abstract: Resveratrol is a phytoalexin synthesized by a wide variety of plants, which has been proven to be effective in suppressing oxidative stress and inflammation. The aim of the present study was to investigate the effect of Resveratrol's prodrug: 3,5,4'-tri-O-acetylresveratrol, on seawater drowning-induced acute lung injury (SWD-ALI). Histological changes were assessed to study lung injuries; cytokines in lung samples were monitored by ELISA to reflect inflammation; T-SOD and MDA activity were detected to examine oxidative stress in lung tissues. Besides, we also tested the expression of NF-κB and HIF-1α to probe the possible protecting mechanism of 3,5,4'-tri-O-acetylresveratrol on AWD-ALI. The results showed that pretreatment with different doses of 3,5,4'-tri-O-acetylresveratrol improved seawater-induced lung histopathologic changes, alleviated lung edema, reduced the production of inflammatory mediators including TNF-α and IL-1β, inhibited MDA activity, and enhanced T-SOD activity, which was possibly associated with inhibition of NF-κB and HIF-1α. In conclusion, the current study demonstrated that 3,5,4'-tri-O-acetylresveratrol exhibited a protective effect on SWD-ALI by inhibiting of the inflammatory response, which may also involve the suppression of oxidative stress in lung tissues.
authors.affiliation: Department of Respiration, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Lijie
authors.initials: L
authors.lastname: Ma
authors.forename: Yilin
authors.initials: Y
authors.lastname: Zhao
authors.forename: Beixi
authors.initials: B
authors.lastname: Li
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Wang
authors.forename: Xueying
authors.initials: X
authors.lastname: Liu
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Chen
authors.forename: Yandong
authors.initials: Y
authors.lastname: Nan
authors.forename: Li
authors.initials: L
authors.lastname: Liang
authors.forename: Ruijing
authors.initials: R
authors.lastname: Chang
authors.forename: Lifeng
authors.initials: L
authors.lastname: Liang
authors.forename: Peipei
authors.initials: P
authors.lastname: Li
authors.forename: Faguang
authors.initials: F
authors.lastname: Jin
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D015153:Blotting, Western; D004332:Drowning / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D055370:Lung Injury / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D011355:Prodrugs / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D012623:Seawater / Q000009:adverse effects; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; C497444:Hif1a protein, rat; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D016328:NF-kappa B; D011355:Prodrugs; D013267:Stilbenes; D000077185:Resveratrol