title: Usefulness of immunoglobulin light-chain restriction on immunocytochemical double staining for the cytological diagnosis of B cell non-Hodgkin's lymphoma.
abstract: OBJECTIVE
  We examined the usefulness of light-chain restriction (LCR) on immunocytochemical double staining (IDS) for cytological diagnosis.
  
  
  METHODS
  We investigated LCR on IDS in 40 patients with proliferative lymphatic disorders (23 with B cell lymphoma, 13 with reactive lymphoid lesions, 2 with T cell lymphoma and 2 with Hodgkin's lymphoma). In addition, the results of flow cytometry (FCM) were compared in 34 of these patients.
  
  
  RESULTS
  On IDS, LCR was detected in 21 of 23 patients (91.3%) with B cell lymphoma. On FCM, it was detected in 15 of 21 patients (71.4%) with B cell lymphoma. Neither IDS nor FCM showed LCR in any patients with reactive lesions, T cell lymphoma or Hodgkin's lymphoma.
  
  
  CONCLUSIONS
  IDS facilitated the detection of LCR with a single specimen under morphological observation. The application of this procedure may improve the accuracy of cytological diagnosis.
authors.affiliation: Department of Pathology, Kagawa Prefectural Central Hospital, Takamatsu, Japan.
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Shimoura
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Sato
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Takata
authors.forename: Yorihisa
authors.initials: Y
authors.lastname: Orita
authors.forename: Satoko
authors.initials: S
authors.lastname: Nakamura
authors.forename: Shyouhei
authors.initials: S
authors.lastname: Mano
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yoshino
mesh_terms: D003581:Cytodiagnosis / Q000379:methods*; D005434:Flow Cytometry; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000032:analysis* / Q000276:immunology; D007150:Immunohistochemistry / Q000379:methods*; D016130:Immunophenotyping; D016393:Lymphoma, B-Cell / Q000175:diagnosis* / Q000276:immunology; D012680:Sensitivity and Specificity
chemical_list: D007147:Immunoglobulin Light Chains