title: Production, preliminary characterization, and bioactivities of exopolysaccharides from Pleurotus geesteranus 5(#).
abstract: The optimal culture conditions of exopolysaccharides (EPS) production in submerged culture medium by Pleurotus geesteranus 5(#) were determined using an orthogonal matrix method. The optimal defined medium (per liter) was 60.0 g maltose, 5.0 g tryptone, 1 mM NaCl, 5 mM KH(2)PO(4), and initial pH 6.0 at 28 °C. In the optimal culture medium, the maximum EPS production was 16.97 g/L in a shake flask. Two groups of EPSs (designated as Fr-I and Fr-II) were obtained from the culture filtrates by size exclusion chromatography (SEC), and their molecular characteristics were examined by a multiangle laser-light scattering (MALLS) and refractive index (RI) detector system. The approximate weight-average molar masses of the Fr-I and Fr-II of EPS were determined to be 3.263 × 10(4) and 5.738 × 10(3) g/mol, respectively. The low values of polydispersity ratio (1.176 and 1.124 for Fr-I and Fr-II, respectively) of EPSs mean that these EPS molecules exist much less dispersed in aqueous solution without forming large aggregates. Furthermore, the experiments in vitro indicated that P. geesteranus 5(#) EPS exhibit high antitumor and antioxidative effects.
authors.affiliation: College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, PR China.
authors.forename: Peixin
authors.initials: P
authors.lastname: He
authors.forename: Lujing
authors.initials: L
authors.lastname: Geng
authors.forename: Duobin
authors.initials: D
authors.lastname: Mao
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.forename: Chunping
authors.initials: C
authors.lastname: Xu
mesh_terms: D000465:Algorithms; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D000975:Antioxidants / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D019149:Bioreactors; D002470:Cell Survival / Q000187:drug effects; D002850:Chromatography, Gel; D003470:Culture Media; D005285:Fermentation; D062610:Fungal Polysaccharides / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D025282:Mycelium / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D020076:Pleurotus / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D025341:Principal Component Analysis; D013696:Temperature
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D003470:Culture Media; D062610:Fungal Polysaccharides