title: Potential probiotic lactic acid bacteria of human origin induce antiproliferation of colon cancer cells via synergic actions in adhesion to cancer cells and short-chain fatty acid bioproduction.
abstract: The activities and modes of probiotic action of lactic acid bacteria isolated from infant feces were investigated for alternative application in the prevention and biotherapy of colon cancer. From a total of 81 isolates of Gram-positive rod and cocci bacteria obtained from healthy infants, only 15 isolates had the probiotic criteria which included growth inhibition against eight food-borne pathogens, no blood hemolysis, and tolerance to gastrointestinal tract properties such as pH 2.5 and 0.3 % bile salt. Four probiotic bacteria showed antiproliferation of colon cancer cells with the use of MTT and Trypan blue exclusion assay at the rates of 17-35 %. Through comparison of probiotic 16S rRNA sequences, they were identified as Pediococcus pentosaceus FP3, Lactobacillus salivarius FP25, L. salivarius FP35, and Enterococcus faecium FP51. Finding the mechanism of proliferative inhibition of colon cancer cells in this study indicated synergic induction by probiotic bacteria directly adhered to these cancer cells and triggered the bioproduction of short-chain fatty acids, mainly butyric and propionic acids. This study suggested that the use of these probiotics may be suitable as an alternative bioprophylactic and biotherapeutic strategy for colon cancer.
authors.affiliation: Division of Biotechnology, Faculty of Science, Maejo University, Chiang Mai 50290, Thailand. mthirabun@yahoo.co.th
authors.forename: Mongkol
authors.initials: M
authors.lastname: Thirabunyanon
authors.forename: Penrat
authors.initials: P
authors.lastname: Hongwittayakorn
mesh_terms: D001422:Bacterial Adhesion; D018938:Caco-2 Cells; D049109:Cell Proliferation / Q000187:drug effects; D005232:Fatty Acids, Volatile / Q000096:biosynthesis*; D005240:Feasibility Studies; D006801:Humans; D007231:Infant, Newborn; D007422:Intestines / Q000382:microbiology*; D007778:Lactobacillus / Q000502:physiology*; D009374:Neoplasms, Experimental / Q000382:microbiology* / Q000628:therapy*; D019936:Probiotics / Q000627:therapeutic use*
chemical_list: D005232:Fatty Acids, Volatile