title: Antifilarial diarylheptanoids from Alnus nepalensis leaves growing in high altitude areas of Uttarakhand, India.
abstract: Lymphatic filariasis continues to be a major health problem in tropical and subtropical countries. A macrofilaricidal agent capable of eliminating adult filarial parasites is urgently needed. Platyphyllenone (A), alusenone (B), hirustenone (C) and hirsutanonol (D) are important biologically active diarylheptanoids present in Alnus nepalensis. In the present study, we report the antifilarial activity in diarylheptanoids isolated from the leaves of A. nepalensis. Out of four compounds (A-D) tested in vitro one has shown promising anti-filarial activity both in vitro and in vivo studies. This is the first ever report on antifilarial efficacy of a compound of the plant and warrants further studies around this scaffold. In addition, a sensitive, selective and robust densitometric high-performance thin-layer chromatographic method was developed and validated for the above four biomarker compounds. The separation was performed on silica gel 60F(254) high-performance thin layer chromatography plates using chloroform:methanol (9:1, v/v) as mobile phase. The quantitation of marker compounds was carried out using densitometric reflection/absorption mode at 600 nm after post-chromatographic derivatization using vanillin-sulfuric acid reagent. The method was validated for peak purity, precision, robustness, limit of detection (LOD) and quantitation (LOQ) etc., as per the International Conference on Harmonization (ICH) guidelines.
authors.affiliation: Analytical Chemistry Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India.
authors.forename: Deepti
authors.initials: D
authors.lastname: Yadav
authors.forename: Subhash Chandra
authors.initials: SC
authors.lastname: Singh
authors.forename: Ram Kishore
authors.initials: RK
authors.lastname: Verma
authors.forename: Kirti
authors.initials: K
authors.lastname: Saxena
authors.forename: Richa
authors.initials: R
authors.lastname: Verma
authors.forename: Puvvada Kalpana
authors.initials: PK
authors.lastname: Murthy
authors.forename: Madan Mohan
authors.initials: MM
authors.lastname: Gupta
mesh_terms: D029661:Alnus / Q000737:chemistry*; D000531:Altitude; D000818:Animals; D017178:Brugia malayi; D036381:Diarylheptanoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D005368:Filariasis / Q000188:drug therapy* / Q000469:parasitology; D005369:Filaricides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005849:Gerbillinae; D007194:India; D008297:Male; D015394:Molecular Structure; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D036381:Diarylheptanoids; D005369:Filaricides; D010936:Plant Extracts; C581537:hirustenone