title: Co-production of two recombinant proteins in Escherichia coli using the promoter region of the divergently expressed curli genes.
abstract: Expression of multiple proteins in a single host is desirable in biotechnological processes. The curli intergenic region in Escherichia coli contains promoter elements for the expression of the divergent csgBAC and csgDEFG operons. Using this bidirectionally active promoter region, we demonstrate high level production of two different recombinant proteins. The curli intergenic region may thus be used to produce multiple enzymes involved in biosynthetic pathways and biotransformations in a cost-effective manner.
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology, Powai, Mumbai 400076, Maharashtra, India.
authors.forename: Shamlan M S
authors.initials: SM
authors.lastname: Reshamwala
authors.forename: Santosh B
authors.initials: SB
authors.lastname: Noronha
mesh_terms: D001709:Biotechnology / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005798:Genes, Bacterial; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D008164:Luminescent Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D000097573:Red Fluorescent Protein
chemical_list: D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D011994:Recombinant Proteins; D000097573:Red Fluorescent Protein