title: Evaluation of a reliable and cost-effective method of DNA isolation for mouse genotyping.
abstract: Genotyping is commonly used to define specific gene alterations or the presence of transgenes in mice. This procedure is typically done using DNA isolated from mouse tail tissue. Although there are commercially available kits for tail DNA isolation, they can be time consuming and costly for routine genotyping. In this study, we describe a rapid, "crude" DNA isolation method using mouse tail tissue and compare it to a frequently used, commercially available kit in the genotyping of over 1,000 total mice from 8 genetic lines. Our genotyping results were obtained faster and less expensively but with the same success rate (Crude method: 97.7 %, Kit method: 98.4 %). To our knowledge, this is the first systematic study to compare the reliability of this crude DNA isolation method for mouse genotyping compared to a commercially available kit.
authors.affiliation: Institute for Personalized Respiratory Medicine, Section of Pulmonary, Critical Care, Sleep and Allergy, Department of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA. jsysol2@uic.edu
authors.forename: Justin R
authors.initials: JR
authors.lastname: Sysol
authors.forename: Carrie
authors.initials: C
authors.lastname: Kempf
authors.forename: Michelle N
authors.initials: MN
authors.lastname: Helton
authors.forename: Yangbasai
authors.initials: Y
authors.lastname: Dong
authors.forename: Daocheng
authors.initials: D
authors.lastname: Zhu
authors.forename: Haiying
authors.initials: H
authors.lastname: Sun
authors.forename: Joe G N
authors.initials: JG
authors.lastname: Garcia
authors.forename: Roberto F
authors.initials: RF
authors.lastname: Machado
authors.forename: Jiwang
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D003365:Costs and Cost Analysis; D004247:DNA / Q000302:isolation & purification*; D005838:Genotype; D051379:Mice; D008967:Molecular Biology / Q000191:economics / Q000379:methods*; D013048:Specimen Handling / Q000191:economics / Q000379:methods*
chemical_list: D004247:DNA