title: Increased Nanog expression promotes tumor development and Cisplatin resistance in human esophageal cancer cells.
abstract: OBJECTIVE
  Nanog plays a key role in stem cell self-renewal and pluripotency differentiation in embryonic stem cells ( ESCs). Recently, some studies reported that abnormal expression of Nanog could be detected in several tumors, indicating that Nanog might be related to tumor development. However, studies on the correlation between Nanog expression and esophageal cancer are sparse.
  
  
  METHODS
  In this study, we established two esophageal cancer cell lines 9706-Nanog and 9706-shNanog which stably expressed Nanog and Nanog-short-hairpin RNA (shRNA) genes.
  
  
  RESULTS
  We found that Nanog expression could promote the proliferation and invasiveness of the cancer cells, and inhibit the apoptosis. We also treated 9706-Nanog, EC9706 and 9706-shNanog cell lines with cisplatin and evaluated the drug sensitivity of the three cell lines. We found that the sensitivity of cisplatin was decreased with increased expression of Nanog. The expression of MDR-1 was also increased in 9706Nanog cells.
  
  
  CONCLUSIONS
  Nanog may play an important role in human esophageal cancer development, and could be used as a therapeutic target in esophageal cancer treatment.
authors.affiliation: Departments of Reproductive Medicine Center, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Xudong
authors.initials: X
authors.lastname: Zhang
authors.forename: Mingzhi
authors.initials: M
authors.lastname: Zhang
authors.forename: Junhui
authors.initials: J
authors.lastname: Zhang
authors.forename: Yuqiao
authors.initials: Y
authors.lastname: Sheng
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Sun
authors.forename: Qingjiang
authors.initials: Q
authors.lastname: Chen
authors.forename: Le-Xin
authors.initials: LX
authors.lastname: Wang
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D004938:Esophageal Neoplasms / Q000188:drug therapy / Q000235:genetics*; D004947:Esophagus / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D000071317:Nanog Homeobox Protein; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000970:Antineoplastic Agents; D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D034741:RNA, Small Interfering; D002945:Cisplatin