title: Characteristics of MIC-1 antlerogenic stem cells and their effect on hair growth in rabbits.
abstract: OBJECTIVE
  We characterized growth factors produced by MIC-1 antlerogenic stem cells and attempted to apply those cells to stimulate hair growth in rabbits.
  
  
  METHODS
  We evaluated the gene and protein expression of growth factors by immunocytochemical and molecular biology techniques in MIC-1 cells. An animal model was used to assess the effects of xenogenous stem cells on hair growth. In the experimental group, rabbits were intradermally injected with MIC-1 stem cells, whereas the control group rabbits were given vehicle-only. After 1, 2 and 4 weeks, skin specimen were collected for histological and immunohistochemical tests.
  
  
  RESULTS
  MIC-1 antlerogenic stem cells express growth factors, as confirmed at the mRNA and protein levels. Histological and immunohistochemical analysis demonstrated an increase in the number of hair follicles, as well as the amount of secondary hair in the follicles, without an immune response in animals injected intradermally with MIC-1 cells, compared to animals receiving vehicle-alone.
  
  
  CONCLUSIONS
  MIC-1 cells accelerated hair growth in rabbits due to the activation of cells responsible for the regulation of the hair growth cycle through growth factors. Additionally, the xenogenous cell implant did not induce immune response.
authors.affiliation: Department of Histology and Embryology, Medical University of Wroclaw, Wroclaw, Poland. marek.cegielski@am.wroc.pl
authors.forename: Marek
authors.initials: M
authors.lastname: Cegielski
authors.forename: Ilona
authors.initials: I
authors.lastname: Izykowska
authors.forename: Magdalena
authors.initials: M
authors.lastname: Chmielewska
authors.forename: Wojciech
authors.initials: W
authors.lastname: Dziewiszek
authors.forename: Marek
authors.initials: M
authors.lastname: Bochnia
authors.forename: Ireneusz
authors.initials: I
authors.lastname: Calkosinski
authors.forename: Piotr
authors.initials: P
authors.lastname: Dziegiel
mesh_terms: D000818:Animals; D000999:Antlers / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D041702:BALB 3T3 Cells; D015153:Blotting, Western; D002460:Cell Line; D003670:Deer; D005260:Female; D015870:Gene Expression; D006197:Hair / Q000254:growth & development*; D018859:Hair Follicle / Q000254:growth & development; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014183:Transplantation, Heterologous
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D016753:Interleukin-10