title: Phaseolus vulgaris RbohB functions in lateral root development.
abstract: Respiratory burst oxidase homologs (RBOHs) catalyze the reduction of oxygen to generate superoxide anion, a kind of reactive oxygen species (ROS). The ROS produced by RBOHs play essential roles in diverse processes, such as root hair development, stomata closure and signaling mechanisms in response to abiotic stimuli and during plant-pathogen interactions. Recently, we found that PvRbohB silencing in transgenic Phaseolus vulgaris roots had a negative impact on lateral root density. In this work, we show that the downregulation of PvRbohB affects both the growth and ROS levels in recently emerged lateral roots. In addition, we found that the PvRbohB promoter was activated during lateral root primordium initiation in the pericycle, and remained active throughout lateral root development. This study identifies RBOHs as potentially important players in lateral root development in P. vulgaris.
authors.affiliation: Departamento de Biología Molecular de Plantas; Instituto de Biotecnología; Universidad Nacional Autónoma de México; Cuernavaca, México.
authors.forename: Jesús
authors.initials: J
authors.lastname: Montiel
authors.affiliation: Departamento de Biología Molecular de Plantas; Instituto de Biotecnología; Universidad Nacional Autónoma de México; Cuernavaca, México.
authors.forename: Manoj-Kumar
authors.initials: MK
authors.lastname: Arthikala
authors.affiliation: Departamento de Biología Molecular de Plantas; Instituto de Biotecnología; Universidad Nacional Autónoma de México; Cuernavaca, México.
authors.forename: Carmen
authors.initials: C
authors.lastname: Quinto
mesh_terms: D015536:Down-Regulation; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism*; D027805:Phaseolus / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D017382:Reactive Oxygen Species / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D017382:Reactive Oxygen Species; D013481:Superoxides; D019255:NADPH Oxidases; C022709:superoxide-forming enzyme