title: Evaluation of P1 adhesin epitopes for the serodiagnosis of Mycoplasma pneumoniae infections.
abstract: Most glycolipid antigens used for serological tests of Mycoplasma pneumoniae are not M. pneumonia-specific, and can cross-react with other microorganism antigens and body tissues, resulting in false positives. It is important to identify M. pneumonia-specific antigen(s) for serological testing and correct diagnosis. Two epitopes, rP1-534 and rP1-513, of P1 adhesin predicted by bioinformatics were successfully expressed and purified, and could be recognized by serum samples from M. pneumoniae-infected patients and His tag antibodies by Western blot. There was no cross-reactivity between the anti-recombinant proteins serum and other respiratory antigens. A total of 400 patients were investigated, their respiratory specimens tested by PCR, and sera tested by a commercial test kit; 56 with positive sera and positive respiratory specimens were designated as standard positive serum and 63 patients were designated as standard negative serum. The purified recombinant proteins were used as a combination of antigens or separately to test the serum. Serological test demonstrated that rP1-513 of the C terminal of P1 adhesin is a new candidate antigen with greater sensitivity and specificity for IgG and IgM serodiagnosis of M. pneumoniae-infected patients. The results confirmed that rP1-513 could be a useful new antigen for the immunodiagnosis of M. pneumoniae infection.
authors.affiliation: Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China.
authors.forename: Guanhua
authors.initials: G
authors.lastname: Xue
authors.forename: Ling
authors.initials: L
authors.lastname: Cao
authors.forename: Luoping
authors.initials: L
authors.lastname: Wang
authors.forename: Hanqing
authors.initials: H
authors.lastname: Zhao
authors.forename: Yanling
authors.initials: Y
authors.lastname: Feng
authors.forename: Lijuan
authors.initials: L
authors.lastname: Ma
authors.forename: Hongmei
authors.initials: H
authors.lastname: Sun
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000276:immunology*; D000293:Adolescent; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003429:Cross Reactions; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009177:Mycoplasma pneumoniae / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D011019:Pneumonia, Mycoplasma / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D012698:Serologic Tests
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000939:Epitopes