title: Single-stranded DNA binding protein Gp5 of Bacillus subtilis phage Φ29 is required for viral DNA replication in growth-temperature dependent fashion.
abstract: In the absence of viral single-stranded DNA binding protein gp5, Bacillus subtilis phage φ29 failed to grow and to replicate its genome at 45 °C, while it grew and replicated normally at 30 °C and 42 °C. This indicates that gp5 is dispensable for φ29 DNA replication at 42 °C and lower temperatures.
authors.affiliation: Laboratory of Genetics, Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Tokyo, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Tone
authors.forename: Ari
authors.initials: A
authors.lastname: Takeuchi
authors.forename: Osamu
authors.initials: O
authors.lastname: Makino
mesh_terms: D017103:Bacillus Phages / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D001412:Bacillus subtilis / Q000821:virology*; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000096:biosynthesis / Q000378:metabolism*; D004279:DNA, Viral / Q000096:biosynthesis* / Q000378:metabolism; D013696:Temperature; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D004277:DNA, Single-Stranded; D004279:DNA, Viral; D014764:Viral Proteins