title: Inhibition of fatty acid synthase suppresses osteosarcoma cell invasion             and migration via downregulation of the PI3K/Akt signaling pathway in vitro.
abstract: In the present study, the effect of fatty acid synthase (FASN) inhibition on cell invasion and migration in vitro was investigated. A recombinant plasmid containing a microRNA targeting the FASN gene was used to inhibit FASN expression in U2‑OS cells. Cell migration and invasion were investigated using wound healing and Transwell invasion assays. We found that cell invasion and migration were suppressed by inhibiting FASN. In addition, the effect of inhibition of FASN on phosphorylation of Akt was investigated by detecting the expression levels of pAkt using western blot analysis. Furthermore, protein expression levels of nuclear factor‑κB (NF‑κB; p65) and matrix metalloproteinase (MMP)‑2 and ‑9 were also measured by western blot analysis. Results demonstrated that expression levels of pAkt, NF‑κB (p65) and MMP‑2 and ‑9 proteins were reduced significantly by inhibiting FASN. Therefore, we confirmed that inhibition of FASN by RNA interference suppresses osteosarcoma cell metastasis via downregulation of the phosphoinositide 3‑kinase/Akt/NF‑κB signaling pathway in vitro.
authors.affiliation: Department of Orthopedics, First Affiliated Hospital of Nanchang                     University, Nanchang 330006, P.R. China.
authors.forename: Zhi Li
authors.initials: ZL
authors.lastname: Liu
authors.forename: Jian Hua
authors.initials: JH
authors.lastname: Mao
authors.forename: Ai Fen
authors.initials: AF
authors.lastname: Peng
authors.forename: Qing Shui
authors.initials: QS
authors.lastname: Yin
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xin Hua
authors.initials: XH
authors.lastname: Long
authors.forename: Shan Hu
authors.initials: SH
authors.lastname: Huang
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D015536:Down-Regulation; D064429:Fatty Acid Synthases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D035683:MicroRNAs / Q000378:metabolism; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D034622:RNA Interference; D015398:Signal Transduction; D051996:Transcription Factor RelA / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D051996:Transcription Factor RelA; D064429:Fatty Acid Synthases; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9