title: Sequential engagement of FcεRI on Mast Cells and Basophil Histamine H(4) Receptor and FcεRI in Allergic Rhinitis.
abstract: Histamine H(4) receptor (H(4)R)-deficient mice (H(4)R(-/-)), H(4)R antagonist-treated wild-type (WT) mice, and WT mice depleted of basophils failed to develop early (EPR) or late phase (LPR) nasal responses following allergen sensitization and challenge. Basophil transfer from WT but not H(4)R(-/-) mice restored the EPR and LPR in H(4)R(-/-) mice. Following passive sensitization with OVA-specific IgE, FcεRI(-/-) recipients of WT basophils plus OVA and histamine developed an EPR and LPR. OVA-IgE passively sensitized FcεRI(-/-) recipients of H(4)R(-/-) basophils and OVA and histamine challenge failed to develop an EPR or LPR, and basophils were not detected in nasal tissue. In contrast, recipients of basophils from IL-13(-/-) and IL-4(-/-)/IL-13(-/-) mice developed an EPR but not an LPR. These results demonstrate the development of allergic rhinitis proceeded in two distinct stages: histamine release from FcεRI-activated mast cells, followed by histamine-mediated recruitment of H(4)R-expressing basophils to the nasal cavity and activation through FcεRI.
authors.affiliation: Division of Cell Biology, Department of Pediatrics, National Jewish Health, Denver, CO 80206, USA.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Shiraishi
authors.forename: Yi
authors.initials: Y
authors.lastname: Jia
authors.forename: Joanne
authors.initials: J
authors.lastname: Domenico
authors.forename: Anthony
authors.initials: A
authors.lastname: Joetham
authors.forename: Hajime
authors.initials: H
authors.lastname: Karasuyama
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Takeda
authors.forename: Erwin W
authors.initials: EW
authors.lastname: Gelfand
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D001491:Basophils / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006633:Histamine Antagonists / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D018448:Models, Immunological; D009297:Nasal Mucosa / Q000187:drug effects / Q000276:immunology; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000276:immunology*; D011968:Receptors, Histamine / Q000235:genetics / Q000276:immunology*; D000074040:Receptors, Histamine H4; D017455:Receptors, IgE / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D065631:Rhinitis, Allergic; D012221:Rhinitis, Allergic, Perennial / Q000235:genetics / Q000276:immunology* / Q000628:therapy
chemical_list: D006633:Histamine Antagonists; C498829:Hrh4 protein, mouse; D043562:Receptors, G-Protein-Coupled; D011968:Receptors, Histamine; D000074040:Receptors, Histamine H4; D017455:Receptors, IgE