title: Tudor domains of the PRC2 components PHF1 and PHF19 selectively bind to histone H3K36me3.
abstract: PRC2 is the major H3K27 methyltransferase and is responsible for maintaining repressed gene expression patterns throughout development. It contains four core components: EZH2, EED, SUZ12 and RbAp46/48 and some cell-type specific components. In this study, we focused on characterizing the histone binding domains of PHF1 and PHF19, and found that the Tudor domains of PHF1 and PHF19 selectively bind to histone H3K36me3. Structural analysis of these Tudor domains also shed light on how these Tudor domains selectively bind to histone H3K36me3. The histone H3K36me3 binding by the Tudor domains of PHF1, PHF19 and likely MTF2 provide another recruitment and regulatory mechanism for the PRC2 complex. In addition, we found that the first PHD domains of PHF1 and PHF19 do not exhibit histone H3K4 binding ability, nor do they affect the Tudor domain binding to histones.
authors.affiliation: Structural Genomics Consortium, University of Toronto, 101 College St., Toronto, Ontario M5G 1L7, Canada.
authors.forename: Su
authors.initials: S
authors.lastname: Qin
authors.forename: Yahong
authors.initials: Y
authors.lastname: Guo
authors.forename: Chao
authors.initials: C
authors.lastname: Xu
authors.forename: Chuanbing
authors.initials: C
authors.lastname: Bian
authors.forename: Minfei
authors.initials: M
authors.lastname: Fu
authors.forename: Sarah
authors.initials: S
authors.lastname: Gong
authors.forename: Jinrong
authors.initials: J
authors.lastname: Min
mesh_terms: D000595:Amino Acid Sequence; D004268:DNA-Binding Proteins / Q000737:chemistry*; D006657:Histones / Q000737:chemistry*; D006801:Humans; D008715:Methionine / Q000737:chemistry; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry*; D063151:Polycomb Repressive Complex 2 / Q000737:chemistry*; D063146:Polycomb-Group Proteins; D017434:Protein Structure, Tertiary; D014157:Transcription Factors / Q000737:chemistry*
chemical_list: D004268:DNA-Binding Proteins; D006657:Histones; C570567:MTF2 protein, human; D009687:Nuclear Proteins; C112309:PHF1 protein, human; C497703:PHF19 protein, human; D063146:Polycomb-Group Proteins; D014157:Transcription Factors; D008715:Methionine; D063151:Polycomb Repressive Complex 2