title: mMaple: a photoconvertible fluorescent protein for use in multiple imaging modalities.
abstract: Recent advances in fluorescence microscopy have extended the spatial resolution to the nanometer scale. Here, we report an engineered photoconvertible fluorescent protein (pcFP) variant, designated as mMaple, that is suited for use in multiple conventional and super-resolution imaging modalities, specifically, widefield and confocal microscopy, structured illumination microscopy (SIM), and single-molecule localization microscopy. We demonstrate the versatility of mMaple by obtaining super-resolution images of protein organization in Escherichia coli and conventional fluorescence images of mammalian cells. Beneficial features of mMaple include high photostability of the green state when expressed in mammalian cells and high steady state intracellular protein concentration of functional protein when expressed in E. coli. mMaple thus enables both fast live-cell ensemble imaging and high precision single molecule localization for a single pcFP-containing construct.
authors.affiliation: Biophysics Graduate Group, University of California, Berkeley, California, United States of America. almcevoy@berkeley.edu
authors.forename: Ann L
authors.initials: AL
authors.lastname: McEvoy
authors.forename: Hiofan
authors.initials: H
authors.lastname: Hoi
authors.forename: Mark
authors.initials: M
authors.lastname: Bates
authors.forename: Evgenia
authors.initials: E
authors.lastname: Platonova
authors.forename: Paula J
authors.initials: PJ
authors.lastname: Cranfill
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Baird
authors.forename: Michael W
authors.initials: MW
authors.lastname: Davidson
authors.forename: Helge
authors.initials: H
authors.lastname: Ewers
authors.forename: Jan
authors.initials: J
authors.lastname: Liphardt
authors.forename: Robert E
authors.initials: RE
authors.lastname: Campbell
mesh_terms: D000818:Animals; D002477:Cells / Q000648:ultrastructure; D004926:Escherichia coli / Q000648:ultrastructure; D005456:Fluorescent Dyes / Q000737:chemistry*; D008027:Light; D008164:Luminescent Proteins / Q000737:chemistry*; D008322:Mammals; D008856:Microscopy, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D008164:Luminescent Proteins