title: FRET-capture: a sensitive method for the detection of dynamic protein interactions.
abstract: Caught in the act: The FRET-Capture approach exploits a bound solvatochromic fluorophore, 4-N,N-dimethylamino-1,8-naphthalimide, as a FRET donor in both inter- and intramolecular energy transfer. A unique feature of this method is the additional level of signal selectivity as the FRET signal is only turned on when the donor is specifically bound to the protein of interest, eliminating high background and false positive signals.
authors.affiliation: Department of Biology and Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
authors.forename: Elke
authors.initials: E
authors.lastname: Socher
authors.forename: Barbara
authors.initials: B
authors.lastname: Imperiali
mesh_terms: D002147:Calmodulin / Q000737:chemistry / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism; D008958:Models, Molecular; D053644:Naphthalimides / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D011487:Protein Conformation; D025941:Protein Interaction Mapping / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: C533757:4-N,N-dimethylamino-1,8-naphthalimide; D002147:Calmodulin; D005456:Fluorescent Dyes; D053644:Naphthalimides; D010446:Peptide Fragments