title: Steroid 5α-reductase in adult rat brain after neonatal dihydrotestosterone administration.
abstract: Testosterone (T) is known to play an important masculinizing role in the developing brain of rat, including the regulation of 5α-reductase (5α-R) isozymes. However, the effects of dihydrotesterone (DHT), a more potent androgen than T, have not been elucidated. In this study, DHT was administered from day 5 through day 20 of postnatal life (period of postnatal sexual differentiation of the central nervous system) at doses of: 12 mg/kg/d on days 5, 6, 7, 8, 19, and 20; 15 mg/kg/d on days 9, 10, 11, 12, 16, 17, and 18; and 18 mg/kg/d on days 13, 14, and 15. In adulthood, quantitative RT-PCR was used to measure mRNA levels of 5α-R1 and 5α-R2 isozymes in the prefrontal cortex (PFC) of male and female rats with varied androgenic status. Under our study conditions, neonatal DHT administration influenced on adult PFC 5α-R isozymes levels and their regulation pattern by androgens, and this pattern was the inverse of that reported in adult neonatally T-treated rats.
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Medicine, University of Granada, Avda. de Madrid s/n, 18012, Granada, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Sánchez
authors.forename: J M
authors.initials: JM
authors.lastname: Torres
authors.forename: B
authors.initials: B
authors.lastname: Castro
authors.forename: R G
authors.initials: RG
authors.lastname: Del Moral
authors.forename: J
authors.initials: J
authors.lastname: de Dios Luna
authors.forename: E
authors.initials: E
authors.lastname: Ortega
mesh_terms: D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000201:enzymology*; D013196:Dihydrotestosterone / Q000494:pharmacology*; D005260:Female; D007527:Isoenzymes / Q000096:biosynthesis; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012733:Sex Differentiation / Q000187:drug effects
chemical_list: D007527:Isoenzymes; D013196:Dihydrotestosterone; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase