title: Proliferation and osteoblastic differentiation of hMSCs on cellulose-based hydrogels.
abstract: OBJECTIVE
  The aim of this project was to study the proliferation and differentiation of human Mesenchymal Stem Cells (hMSCs) onto a cellulose-based hydrogel for bone tissue engineering.
  
  
  METHODS
  Modified-cellulose hydrogel was prepared via double esterification crosslinking using citric acid. The response of human Mesenchymal Stem Cells (hMSCs) in terms of cell proliferation and differentiation into osteoblastic phenotype was evaluated by using Alamar blue assay and Alkaline phosphatase activity.
  
  
  RESULTS
  The results showed that CMCNa and CMCNa_CA have no negative effect on hMSC, adhesion and proliferation. Moreover, the increase of the ALP expression for CMCNa_CA confirms the ability of the hydrogels to support the osteoblastic differentiation.
  
  
  CONCLUSIONS
  The cellulose-based hydrogels have a potential application as filler in bone tissue regeneration.
authors.affiliation: Institute of Composite and Biomedical Materials, National Research Council of Italy, Naples, Italy. mariagrazia.raucci@cnr.it
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Raucci
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: Alvarez-Perez
authors.forename: Christian
authors.initials: C
authors.lastname: Demitri
authors.forename: Alessandro
authors.initials: A
authors.lastname: Sannino
authors.forename: Luigi
authors.initials: L
authors.lastname: Ambrosio
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001861:Bone Regeneration; D001842:Bone and Bones / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002482:Cellulose / Q000737:chemistry*; D019343:Citric Acid / Q000737:chemistry; D004951:Esterification; D006801:Humans; D020100:Hydrogels / Q000737:chemistry* / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis; D010641:Phenotype; D023822:Tissue Engineering
chemical_list: D020100:Hydrogels; D019343:Citric Acid; D002482:Cellulose; D000469:Alkaline Phosphatase