title: Expression analysis of iPS cell - inductive genes in esophageal squamous cell carcinoma by tissue microarray.
abstract: OBJECTIVE
  To understand the role of iPS inductive genes in esophageal cancer, we examined the expression of Sex determining region Y-box 2 (SOX2), Octamer-binding transcription factor 3/4 (OCT3/4), Krueppel-like factor 4 (KLF4), c-Myelocytomatosis viral oncogene (c-MYC) and Tir Na Nog (NANOG) using an esophageal squamous cell carcinoma tissue micrroarray.
  
  
  METHODS
  The immunohistochemical expression levels of the five genes were compared to the clinicopathological data of the 81 patients with esophageal cancer.
  
  
  RESULTS
  There was no relationship between the expression of the five genes and TNM factors of the patients. High expression of NANOG was an independent favorable prognostic factor (p=0.041). Among the patients who received postoperative cisplatin-based chemotherapy, patients with NANOG-positive tumor had significantly better prognosis than those whose tumors were NANOG negative (p=0.024). On the other hand, those with c-MYC-positive expression tended to have a worse prognosis and were resistant to cisplatin-based chemotherapy.
  
  
  CONCLUSIONS
  NANOG expression was found to be an independent prognostic factor for patient with esophageal cancer. Patients with NANOG-positive expression tumor may be good candidates for cisplatin-based treatment.
authors.affiliation: Department of Surgery & Science, Graduate School of Medicine & Pharmaceutical Sciences for Research, University of Toyama, Sugitani 2630, Toyama, 930-0194, Japan. yshimada@med.u-toyama.ac.jp
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Shimada
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Okumura
authors.forename: Shinichi
authors.initials: S
authors.lastname: Sekine
authors.forename: Makoto
authors.initials: M
authors.lastname: Moriyama
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Sawada
authors.forename: Koshi
authors.initials: K
authors.lastname: Matsui
authors.forename: Isaku
authors.initials: I
authors.lastname: Yoshioka
authors.forename: Shozo
authors.initials: S
authors.lastname: Hojo
authors.forename: Toru
authors.initials: T
authors.lastname: Yoshida
authors.forename: Takuya
authors.initials: T
authors.lastname: Nagata
authors.forename: Junya
authors.initials: J
authors.lastname: Fukuoka
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Tsukada
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D057026:Induced Pluripotent Stem Cells / Q000502:physiology*; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D008875:Middle Aged; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis / Q000235:genetics; D011379:Prognosis; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis / Q000235:genetics; D055748:SOXB1 Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D046888:Tissue Array Analysis
chemical_list: D018398:Homeodomain Proteins; C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; C489427:MYC protein, human; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D016271:Proto-Oncogene Proteins c-myc; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors