title: Bone regeneration by polyhedral microcrystals from silkworm virus.
abstract: Bombyx mori cypovirus is a major pathogen which causes significant losses in silkworm cocoon harvests because the virus particles are embedded in micrometer-sized protein crystals called polyhedra and can remain infectious in harsh environmental conditions for years. But the remarkable stability of polyhedra can be applied on slow-release carriers of cytokines for tissue engineering. Here we show the complete healing in critical-sized bone defects by bone morphogenetic protein-2 (BMP-2) encapsulated polyhedra. Although absorbable collagen sponge (ACS) safely and effectively delivers recombinant human BMP-2 (rhBMP-2) into healing tissue, the current therapeutic regimens release rhBMP-2 at an initially high rate after which the rate declines rapidly. ACS impregnated with BMP-2 polyhedra had enough osteogenic activity to promote complete healing in critical-sized bone defects, but ACS with a high dose of rhBMP-2 showed incomplete bone healing, indicating that polyhedral microcrystals containing BMP-2 promise to advance the state of the art of bone healing.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Kanagawa Dental College, 82 Inaoka, Yokosuka, Kanagawa 238-8580, Japan.
authors.forename: Goichi
authors.initials: G
authors.lastname: Matsumoto
authors.forename: Takayo
authors.initials: T
authors.lastname: Ueda
authors.forename: Junko
authors.initials: J
authors.lastname: Shimoyama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ijiri
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Omi
authors.forename: Hisato
authors.initials: H
authors.lastname: Yube
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Sugita
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Kubo
authors.forename: Hatsuhiko
authors.initials: H
authors.lastname: Maeda
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Kinoshita
authors.forename: Duverney Gaviria
authors.initials: DG
authors.lastname: Arias
authors.forename: Junji
authors.initials: J
authors.lastname: Shimabukuro
authors.forename: Eiji
authors.initials: E
authors.lastname: Kotani
authors.forename: Shin
authors.initials: S
authors.lastname: Kawamata
authors.forename: Hajime
authors.initials: H
authors.lastname: Mori
mesh_terms: D000818:Animals; D012831:Bombyx / Q000821:virology*; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage; D001842:Bone and Bones / Q000502:physiology*; D003460:Crystallization; D006801:Humans; D012038:Regeneration; D012087:Reoviridae / Q000502:physiology*; D023822:Tissue Engineering
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2