title: Vancomycin-resistant gram-positive cocci isolated from the saliva of wild songbirds.
abstract: We analyzed highly vancomycin-resistant Gram-positive bacteria isolated from the saliva of migratory songbirds captured, sampled, and released from a bird-banding station in western Kansas. Individual bacterial isolates were identified by partial 16S rRNA sequencing. Most of the bacteria in this study were shown to be Staphylococcus succinus with the majority being isolated from the American Robin. Some of these bacteria were shown to carry vanA, vanB, and vanC vancomycin-resistance genes and have the ability to form biofilms. One of the van gene-carrying isolates is also coagulase positive, which is normally considered a virulence factor. Other organisms isolated included Staphylococcus saprophyticus as well as Enterococcus gallinarum. Given the wide range of the American Robin and ease of horizontal gene transfer between Gram-positive cocci, we postulate that these organisms could serve as a reservoir of vancomycin-resistance genes capable of transferring to human pathogens.
authors.affiliation: Department of Biological Sciences, Fort Hays State University, 600 Park Street, Hays, KS 67601, USA.
authors.forename: Shingo
authors.initials: S
authors.lastname: Ishihara
authors.forename: Jessica J
authors.initials: JJ
authors.lastname: Bitner
authors.forename: Greg H
authors.initials: GH
authors.lastname: Farley
authors.forename: Eric T
authors.initials: ET
authors.lastname: Gillock
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016983:Enterococcus / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D007615:Kansas; D012463:Saliva / Q000382:microbiology*; D020308:Songbirds / Q000382:microbiology*; D013210:Staphylococcus / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D014640:Vancomycin / Q000494:pharmacology; D020713:Vancomycin Resistance
chemical_list: D000900:Anti-Bacterial Agents; D014640:Vancomycin