title: Sensitive and convenient detection of microRNAs based on cascade amplification by catalytic DNAzymes.
abstract: On target: We have developed two cascade amplification strategies that combine duplex specific nuclease (DSN) amplicon with either G-quadruplex-based DNA peroxidase or 8-17 DNAzyme amplicon for miRNA detection. In this way, sensitive and convenient detection of miRNAs was achieved. In the DNA peroxidase-based system, a visual color change could be observed in the presence of target miRNAs (see scheme).
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei, 430072, PR China.
authors.forename: Tian
authors.initials: T
authors.lastname: Tian
authors.forename: Heng
authors.initials: H
authors.lastname: Xiao
authors.forename: Zhengan
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yuelin
authors.initials: Y
authors.lastname: Long
authors.forename: Shuang
authors.initials: S
authors.lastname: Peng
authors.forename: Shaoru
authors.initials: S
authors.lastname: Wang
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
authors.forename: Songmei
authors.initials: S
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
mesh_terms: D002384:Catalysis; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D021881:DNA, Catalytic / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000008:administration & dosage* / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009696:Nucleic Acids; D010545:Peroxides / Q000737:chemistry* / Q000378:metabolism
chemical_list: C057755:DNA hydroperoxide; D021881:DNA, Catalytic; C533895:MIRN141 microRNA, human; D035683:MicroRNAs; D009696:Nucleic Acids; D010545:Peroxides; D004247:DNA