title: Colorimetric detection of UV light-induced single-strand DNA breaks using gold nanoparticles.
abstract: We developed a colorimetric method to specifically detect single-strand DNA breaks using gold nanoparticles. In our assay, broken DNA cannot stabilize gold nanoparticles to prevent salt-induced aggregation as good as intact DNA can, and this effect can be easily observed with the naked eye as a red-to-purple color change.
authors.affiliation: BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 125, Gwahak-ro, Yuseong-gu, Daejeon 305-806, South Korea.
authors.forename: Joong Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Chan Ho
authors.initials: CH
authors.lastname: Chung
authors.forename: Bong Hyun
authors.initials: BH
authors.lastname: Chung
mesh_terms: D001128:Argon / Q000737:chemistry; D003124:Colorimetry; D004247:DNA / Q000032:analysis*; D053904:DNA Breaks, Single-Stranded / Q000528:radiation effects*; D006046:Gold / Q000737:chemistry*; D007660:Ketoprofen / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D012492:Salts / Q000737:chemistry; D014466:Ultraviolet Rays
chemical_list: D012492:Salts; D001128:Argon; D006046:Gold; D004247:DNA; D007660:Ketoprofen