title: Metagenomics-based analysis of viral communities in dairy lagoon wastewater.
abstract: Microbial populations, especially those of viruses, are poorly studied in dairy wastewater treatment operations. Here we report signature nucleic acid metagenomic sequences obtained by pyrosequencing viromes of virus-like particles that were extracted from two dairy waste treatment lagoons. The lagoons are operated in series, with Lagoon I being used as the primary stage and Lagoon II as the secondary stage of wastewater treatment. An average of 2000 sequences was obtained from each lagoon. More than 300 signatures from each lagoon matched sequences in the virus database of the National Center for Biotechnology Information (NCBI). We utilized a bioinformatics approach and transmission electron microscopy (TEM) to characterize the viral diversity and presence of potential viral pathogens within the lagoons. Our results showed differences in viral community compositions between Lagoon I and Lagoon II, suggesting that the viral community changes significantly in the transition of water between the two lagoons. Furthermore, the diverse viral community in the lagoon samples contained signature sequences of a variety of bacterial, plant, and animal viruses. Bacteriophage sequences dominated the viral community metagenomes in both lagoons. Ultimately these results can be used to identify viral bioindicators to rapidly assess wastewater treatment quality and the potential impacts of dairy operations on watersheds. Our viral metagenomic sequences have been submitted to GenBank (GPID 65805) and can provide insight into the composition and structure of viral communities within wastewaters of dairy lagoon systems.
authors.affiliation: Department of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Life Science 142 PO Box 443052. Moscow, ID 83844-3052, USA.
authors.forename: F S
authors.initials: FS
authors.lastname: Alhamlan
authors.forename: M M
authors.initials: MM
authors.lastname: Ederer
authors.forename: C J
authors.initials: CJ
authors.lastname: Brown
authors.forename: E R
authors.initials: ER
authors.lastname: Coats
authors.forename: R L
authors.initials: RL
authors.lastname: Crawford
mesh_terms: D000818:Animals; D044822:Biodiversity; D019295:Computational Biology; D019649:Food Industry; D056186:Metagenomics / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D014780:Viruses / Q000145:classification* / Q000235:genetics* / Q000648:ultrastructure; D062065:Wastewater / Q000821:virology*
chemical_list: D062065:Wastewater