title: Comparison of 2 blood culture media shows significant differences in bacterial recovery for patients on antimicrobial therapy.
abstract: BACKGROUND
  Antimicrobial removal devices in blood culture media are designed to remove antibiotics from the blood culture solution, thereby facilitating bacterial growth. How well these devices function clinically has not been established.
  
  
  METHODS
  All blood drawn for culture from adult inpatients and emergency department visitors in a level I trauma center was placed in paired BACTEC Plus and BacT/Alert FAN culture media and studied simultaneously, consecutively, and prospectively between 1 February and 30 September 2011. All cultures were processed per standard laboratory protocols.
  
  
  RESULTS
  Of 9395 total cultures collected, 1219 (13%) were positive, 831 were included, and 524 (33%) contained pathogens. BACTEC had a 4.5-hour faster detection time (P < .0001), and isolated exclusively 182 of 524 (35%; P < .001) pathogens, 136 of 345 (39%) of the gram-positive cocci (P < .001), 48 of 175 (27%; P = .02) of the gram-negative rods, 101 of 195 (52%) of Staphylococcus aureus (P < .001), and 59 of 120 (49%; P = .004) septic events. If active antibiotics had been dosed 0-4 or 4-48 hours prior to culture collection, the odds of that culture growing in BACTEC were 4.8- and 5.2-fold greater, respectively, than of growing in BacT/Alert (P < .0001). Both were equivalent in the recovery of yeast and when no antimicrobials were dosed.
  
  
  CONCLUSIONS
  BACTEC media has faster time to detection and increased bacterial recovery over the BacT/Alert media in the following categories: overall growth, pathogens, septic events, gram-positive cocci, gram-negative rods, Staphylococcus aureus, and cultures where antimicrobials were dosed up to 48 hours before culture collection.
authors.affiliation: Veterans Affairs Medical Center, University of Minnesota, Minneapolis, USA.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Zadroga
authors.forename: David N
authors.initials: DN
authors.lastname: Williams
authors.forename: Richard
authors.initials: R
authors.lastname: Gottschall
authors.forename: Kevan
authors.initials: K
authors.lastname: Hanson
authors.forename: Vickie
authors.initials: V
authors.lastname: Nordberg
authors.forename: Marcia
authors.initials: M
authors.lastname: Deike
authors.forename: Mike
authors.initials: M
authors.lastname: Kuskowski
authors.forename: Lisa
authors.initials: L
authors.lastname: Carlson
authors.forename: David P
authors.initials: DP
authors.lastname: Nicolau
authors.forename: Christina
authors.initials: C
authors.lastname: Sutherland
authors.forename: Glen T
authors.initials: GT
authors.lastname: Hansen
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000037:antagonists & inhibitors* / Q000627:therapeutic use*; D016470:Bacteremia / Q000175:diagnosis*; D001419:Bacteria / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media