title: DNA-binding, photocleavage studies of ruthenium(II) complexes with 2-(2-quinolinyl) imidazo[4,5-f][1,10]phenanthroline.
abstract: Two new ruthenium complexes with [Ru(L)(2)(qip)](2+) (L=bpy (2,2'- bipyridine), phen (1,10-phenanthroline); qip=2-(2-quinolinyl)imidazo[4,5-f][1,10]phenanthroline), have been synthesized and characterized by elemental analysis, ES-MS, (1)H NMR. The binding properties of two complexes towards CT-DNA were investigated by various optical methods and viscosity measurements. The experiment results suggested that both Ru(II) complexes can intercalate into DNA base pairs. Strong quenching in emission intensity of two Ru(II) complexes were observed upon addition of Ag(+) in the absence and presence of CT-DNA. Furthermore, the two complexes can promote cleavage of pBR322 DNA under irradiation at 365 nm, and complex 2 exhibits a stronger DNA-photocleavage efficiency than complex 1. The mechanism of DNA cleavage suggests that singlet oxygen ((1)O(2)) is likely to be the cleaving agent.
authors.affiliation: College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, PR China. liuxuewen050@sina.com
authors.forename: Xue-Wen
authors.initials: XW
authors.lastname: Liu
authors.forename: Zhen-Gan
authors.initials: ZG
authors.lastname: Chen
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Yuan-Dao
authors.initials: YD
authors.lastname: Chen
authors.forename: Ji-Lin
authors.initials: JL
authors.lastname: Lu
authors.forename: Da-Shun
authors.initials: DS
authors.lastname: Zhang
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D053837:DNA Cleavage / Q000187:drug effects / Q000528:radiation effects; D007093:Imidazoles / Q000737:chemistry / Q000494:pharmacology; D007364:Intercalating Agents / Q000737:chemistry* / Q000494:pharmacology*; D008163:Luminescent Measurements; D009942:Organometallic Compounds / Q000737:chemistry* / Q000494:pharmacology*; D010618:Phenanthrolines / Q000737:chemistry* / Q000494:pharmacology*; D055668:Photochemical Processes; D012834:Silver / Q000378:metabolism
chemical_list: D007093:Imidazoles; D007364:Intercalating Agents; D009942:Organometallic Compounds; D010618:Phenanthrolines; C103599:bis(bipyridyl)ruthenium(II); D012834:Silver; D015082:2,2'-Dipyridyl; D004247:DNA; C427203:calf thymus DNA; C025205:1,10-phenanthroline