title: Fluorescence imaging of synapse formation and remodeling.
abstract: Brain function is based on proper connectivity between neuronal cells. In the developing brain, neurons extend axons and form synaptic connections with appropriate postsynaptic neurons. Molecular mechanisms underlying establishment of proper synaptic connections are one of the most important topics in the field of developmental neurobiology. Dynamics of synaptic structure and local recruitment of synaptic molecules can be studied by live-cell imaging of neurons expressing fluorescent probes of synaptic molecules. In this review, examples of live-cell fluorescence imaging are presented and their contributions to our understanding about the molecular mechanisms of synapse formation and remodeling are discussed. Imaging of synaptic proteins in living neurons revealed rapid formation of individual synapses within hours and extensive remodeling of synaptic connections. Different types of neurons express unique protrusions from dendrites and axons, which play important roles in synapse formation and maturation. Rapid formation of synaptic structure is associated with continual assembly and disassembly of synaptic scaffolding proteins, which are essential building blocks of the presynaptic active zone and the postsynaptic density (PSD). Quantitative analyses of PSD scaffolding proteins further confirmed their essential roles in maintenance of the synaptic structure. These examples clearly indicate that fluorescence-based live-cell imaging is an indispensable technique in the research on synapse development and its impact will further increase in combination with development of new light microscopic techniques in the future.
authors.affiliation: Department of Cellular Neurobiology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, Japan. okabe@m.u-tokyo.ac.jp
authors.forename: Shigeo
authors.initials: S
authors.lastname: Okabe
mesh_terms: D000818:Animals; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D061848:Optical Imaging / Q000379:methods*; D057907:Post-Synaptic Density / Q000378:metabolism / Q000648:ultrastructure; D013569:Synapses / Q000378:metabolism* / Q000648:ultrastructure*