title: [Real-time PCR detection and quantification of emerging waterborne pathogens (EWPs) and antibiotic resistance genes (ARGs) in the downstream area of Jiulong River].
abstract: The emerging waterborne pathogens (EWPs) and antibiotic resistance genes (ARGs) are important for drinking water safety. The detection and quantification of 7 EWPs and 4 ARGs were carried out in Jiulong River, which is the main water source of southwestern Fujian Province. The water samples were collected from four sites of the Jiulong River downstream area and a drinking water treatment plant nearby. DNA was extracted and quantified by real-time (SYBR Green) PCR methods after the samples were filtered through 0.22 pim membranes. The results showed that the amount of Salmonella enterica (Salmonella spp.), Legionella pneumophila (L. pneumophila) and Pseudomonas aeruginosa (P. aeruginosa) could reach up to 10(3), 10(4) and 10(5) copies x mL(-1), respectively. The concentration of organic matter in water may affect the copy numbers significantly. The water plant could effectively remove most EWPs and ARGs except Salmonella spp.. Therefore, more efforts should be made on water pollution source control, water treatment technology and point-of-use system to make sure the safety of drinking water.
authors.affiliation: Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China. qwang@iue.ac.cn
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.forename: Hui-rong
authors.initials: HR
authors.lastname: Lin
authors.forename: Shu-ting
authors.initials: ST
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Yu
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis; D060766:Drinking Water; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016952:Legionella pneumophila / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction; D045483:Rivers; D019779:Salmonella enterica / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D060766:Drinking Water