title: Laboratory capability for molecular detection and confirmation of novel coronavirus in Europe, November 2012.
abstract: A rapid survey by the European Centre for Disease Prevention and Control (ECDC) and the World Health Organization (WHO) Regional Office for Europe ascertained the availability of national reference laboratory testing for a recently detected novel coronavirus as of 28 November 2012. Screening by internal quality controlled upE-RT-PCR assay was available in 23/46 of responding countries in the WHO European Region, of which 19/30 in European Union (EU) and European Economic Area (EEA) countries. Confirmation of positive screened samples by either ORF1b - RT-PCR, or other target RT-PCR assays with sequence analysis or whole-genome sequence analysis was available in 22/46 responding countries of which 18/30 in EU/EEA countries.
authors.affiliation: European Centre for Disease Prevention and Control (ECDC), Stockholm, Sweden.
authors.forename: D
authors.initials: D
authors.lastname: Palm
authors.forename: D
authors.initials: D
authors.lastname: Pereyaslov
authors.forename: J
authors.initials: J
authors.lastname: Vaz
authors.forename: E
authors.initials: E
authors.lastname: Broberg
authors.forename: H
authors.initials: H
authors.lastname: Zeller
authors.forename: D
authors.initials: D
authors.lastname: Gross
authors.forename: C S
authors.initials: CS
authors.lastname: Brown
authors.forename: M J
authors.initials: MJ
authors.lastname: Struelens
mesh_terms: D017934:Coronavirus / Q000235:genetics / Q000302:isolation & purification*; D018352:Coronavirus Infections / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D005062:European Union; D006801:Humans; D007753:Laboratories / Q000592:standards*; D012367:RNA, Viral / Q000235:genetics; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D017421:Sequence Analysis; D014944:World Health Organization
chemical_list: D012367:RNA, Viral