title: [Study on HPLC fingerprints of Chrysanthemi Flos before and after sulfur-fumigation].
abstract: OBJECTIVE
  To evaluate the quality of Chrysanthemi Flos before and after sulfur-fumigation by analysis of HPLC fingerprints.
  
  
  METHODS
  RP-HPLC method was applied to establish the chromatographic fingerprint. The separation was performed on a Waters C, (250 mm x 4.6 mm, 5 microm) column with a gradient elution composed of acetonitrile-aqueous solution containing 0.5% phosphoric acid. The column temperature was 35 degrees C, the detective wavelength was set at 268 nm.
  
  
  RESULTS
  Eleven batches of Chrysanthemi Flos samples composed of both sun-dried and sulfur-fumigated were analyzed and evaluated respectively. The method of clustering analysis was used, and the chromatographic fingerprint common pattern was finally established.
  
  
  CONCLUSIONS
  The HPLC fingerprint method is reliable and suitable for quality evaluation of Chrysanthemi Flos before and after sulfur-fumigation.
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China. liuxiao04_0@163.com
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.forename: Hao
authors.initials: H
authors.lastname: Cai
authors.forename: Xiao-qing
authors.initials: XQ
authors.lastname: Ma
authors.forename: Ke
authors.initials: K
authors.lastname: Pei
authors.forename: Bao-chang
authors.initials: BC
authors.lastname: Cai
mesh_terms: D019659:Asteraceae / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016000:Cluster Analysis; D003890:Desiccation / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry; D005651:Fumigation / Q000379:methods*; D011786:Quality Control; D015203:Reproducibility of Results; D013455:Sulfur / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D013455:Sulfur