title: Multiscale vision model highlights spontaneous glial calcium waves recorded by 2-photon imaging in brain tissue.
abstract: Intercellular glial calcium waves (GCW) constitute a signaling pathway which can be visualized by fluorescence imaging of cytosolic Ca(2+) changes. Reliable detection of calcium waves in multiphoton imaging data is challenging because of low signal-to-noise ratio. We modified the multiscale vision model (MVM), originally employed to detect faint objects in astronomy data to process stacks of fluorescent images. We demonstrate that the MVM identified and characterized GCWs with much higher sensitivity and detail than pixel thresholding. Origins of GCWs were often associated with prolonged secondary Ca(2+) elevations. The GCWs had variable shapes, and secondary GCWs were observed to bud from the primary, larger GCW. GCWs evaded areas shortly before occupied by a preceding GCW instead circulating around the refractory area. Blood vessels uniquely reshaped GCWs and were associated with secondary GCW events. We conclude that the MVM provides unique possibilities to study spatiotemporally correlated Ca(2+) signaling in brain tissue.
authors.affiliation: Department of Biophysics, Faculty of Biology, Moscow State University, 119234 Moscow, Russia. brazhe@biophys.msu.ru
authors.forename: Alexey
authors.initials: A
authors.lastname: Brazhe
authors.forename: Claus
authors.initials: C
authors.lastname: Mathiesen
authors.forename: Martin
authors.initials: M
authors.lastname: Lauritzen
mesh_terms: D000465:Algorithms; D001921:Brain / Q000378:metabolism*; D001931:Brain Mapping / Q000379:methods*; D002118:Calcium / Q000378:metabolism*; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D009457:Neuroglia / Q000378:metabolism*; D017785:Photons
chemical_list: D002118:Calcium