title: A coxsackievirus B5-associated aseptic meningitis outbreak in Shandong Province, China in 2009.
abstract: In 2009, a major outbreak of aseptic meningitis was noted in Linyi city, Shandong province, China. From June to September 2009, a total of 2,104 cases were involved in this outbreak, and 98.6% of patients were <16 years of age. To determine the pathogen of the outbreak, 42 cerebrospinal fluid specimens collected from aseptic meningitis cases were tested for cell culture, and 17 (40.5%) enteroviruses were isolated and identified as Coxsackievirus B5 (CVB5). Homologous comparison indicated that these isolates had 0-7.7% nucleotide divergence with each other. Phylogenetic reconstruction showed global CVB5 could be separated into four genogroups, and all Linyi CVB5 isolates belonged to the genogroup C which had been circulating for recent 27 years in Asia and Europe. Interestingly, two distinct lineages were observed for the 17 isolates in the phylogenetic tree, indicating that at least two different transmission chains of CVB5 were responsible for this outbreak. This study showed that CVB5-associated aseptic meningitis is an emerging concern in China.
authors.affiliation: Department of Epidemiology, School of Public Health, Shandong University, Jinan, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.forename: Zexin
authors.initials: Z
authors.lastname: Tao
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Song
authors.forename: Guifang
authors.initials: G
authors.lastname: Liu
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.forename: Yao
authors.initials: Y
authors.lastname: Liu
authors.forename: Lizhi
authors.initials: L
authors.lastname: Song
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Lin
authors.forename: Ning
authors.initials: N
authors.lastname: Cui
authors.forename: Aiqiang
authors.initials: A
authors.lastname: Xu
mesh_terms: D000293:Adolescent; D000328:Adult; D002555:Cerebrospinal Fluid / Q000821:virology; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D003384:Coxsackievirus Infections / Q000453:epidemiology* / Q000821:virology*; D004196:Disease Outbreaks; D029822:Enterovirus B, Human / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008582:Meningitis, Aseptic / Q000453:epidemiology* / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D014776:Virus Cultivation; D055815:Young Adult
chemical_list: D012367:RNA, Viral