title: Bulked segregant  analysis for relative water content to detect quantitative trait loci in wheat  under drought stress.
abstract: This  study was carried out to evaluate the genetic effect of quantitative trait loci  (QTLs) conferring drought tolerance in wheat. A population of 120 F(2) individuals from  the cross between the drought-tolerant S-78-11 and drought-sensitive Tajan  cultivars were analyzed for their segregation under drought stress conditions.  The relative water content under drought stress conditions exhibited continuous  variation, indicating the minor gene effects on the trait. Single-marker  analysis (SMA) was carried out to detect the main QTL association with drought  tolerance. The SMA results revealed that the simple sequence repeat markers  GWM182 and GWM292 on chromosome 5D and GWM410 on chromosome 5A exhibited  significant association with drought tolerance, accounting for 30, 22, and 21%  of the total variation, respectively. The 3 genetic loci, especially GWM182,  can be used in marker-assisted selection methods in drought tolerance breeding  in wheat.
authors.affiliation: Agrotechnology Department, Faculty of Agriculture, University Putra Malaysia, Serdang, Selangor, Malaysia. narouirad@gmail.com
authors.forename: M R
authors.initials: MR
authors.lastname: Naroui Rad
authors.forename: M
authors.initials: M
authors.lastname: Abdul Kadir
authors.forename: M Y
authors.initials: MY
authors.lastname: Rafii
authors.forename: H Z E
authors.initials: HZ
authors.lastname: Jaafar
authors.forename: M R
authors.initials: MR
authors.lastname: Naghavi
mesh_terms: D000704:Analysis of Variance; D020090:Chromosome Segregation / Q000235:genetics*; D003433:Crosses, Genetic; D055864:Droughts; D005819:Genetic Markers; D018895:Microsatellite Repeats / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics*; D014908:Triticum / Q000235:genetics* / Q000502:physiology*; D014867:Water / Q000378:metabolism*
chemical_list: D005819:Genetic Markers; D014867:Water