title: [Determination of five components in Pueraria labta decoction with reference extraction method].
abstract: OBJECTIVE
  To establish the reference extraction method for determining five components contained in Pueraria labta decoction and detecting the applicability and feasibility of the application of reference extracts according to the quality standard of traditional Chinese medicine.
  
  
  METHODS
  Zorbax C18 chromatographic column (4.6 mm x 250 mm, 5 microm) was used, with methanol-0.02% phosphoric acid (25:75) as the mobile phase and the detection wavelength was set at 250 nm. Self-prepared reference extracts of P. labta was used for determining the content of 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, isoflavoues aglycone-8-C-apiose-(1--> 6) glucoside and daidzin and compare with the experimental results obtained with the single reference substance determination method.
  
  
  RESULTS
  The reference extraction determination method showed high precision, stability and reproducibility, with coincident determination results with the results obtained with the single reference substance determination method.
  
  
  CONCLUSIONS
  The reference extraction determination method of P. labta can be used for quality control of P. labta herbs. The study preliminarily proves the feasibility of the application of P. labta in traditional Chinese medicine.
authors.affiliation: Institute of Chinese Materia, Medica China Academy of Chinese Medical Sciences, Beijing 100700, China. beibei4586@163.com
authors.forename: Cuijuan
authors.initials: C
authors.lastname: Xue
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Wenguang
authors.initials: W
authors.lastname: Jing
authors.forename: Yuesheng
authors.initials: Y
authors.lastname: Wang
authors.forename: Nan
authors.initials: N
authors.lastname: Si
authors.forename: An
authors.initials: A
authors.lastname: Liu
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000302:isolation & purification*; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification; D007529:Isoflavones / Q000032:analysis / Q000302:isolation & purification; D029908:Pueraria / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D005960:Glucosides; D007529:Isoflavones; C033607:puerarin