title: Minocycline reduces reactive gliosis in the rat model of hydrocephalus.
abstract: BACKGROUND
  Reactive gliosis had been implicated in injury and recovery patterns associated with hydrocephalus. Our aim is to determine the efficacy of minocycline, an antibiotic known for its anti-inflammatory properties, to reduce reactive gliosis and inhibit the development of hydrocephalus.
  
  
  RESULTS
  The ventricular dilatation were evaluated by MRI at 1-week post drugs treated, while GFAP and Iba-1were detected by RT-PCR, Immunohistochemistry and Western blot. The expression of GFAP and Iba-1 was significantly higher in hydrocephalic group compared with saline control group (p < 0.05). Minocycline treatment of hydrocephalic animals reduced the expression of GFAP and Iba-1 significantly (p < 0.05). Likewise, the severity of ventricular dilatation is lower in minocycline treated hydrocephalic animals compared with the no minocycline group (p < 0.05).
  
  
  CONCLUSIONS
  Minocycline treatment is effective in reducing the gliosis and delaying the development of hydrocephalus with prospective to be the auxiliary therapeutic method of hydrocephalus.
authors.affiliation: Department of Neurosurgery, First Affiliate Hospital of Xiamen University, Xiamen, Fujian Province 361003, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.forename: Guowei
authors.initials: G
authors.lastname: Tan
authors.forename: Shaolin
authors.initials: S
authors.lastname: Zhang
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhu
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Caiquan
authors.initials: C
authors.lastname: Huang
authors.forename: Feifei
authors.initials: F
authors.lastname: Zhang
authors.forename: Zhanxiang
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism; D002552:Cerebral Ventricles / Q000187:drug effects / Q000473:pathology; D004195:Disease Models, Animal; D004311:Double-Blind Method; D005786:Gene Expression Regulation / Q000187:drug effects*; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics / Q000378:metabolism; D005911:Gliosis / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D006849:Hydrocephalus / Q000150:complications / Q000188:drug therapy / Q000401:mortality / Q000473:pathology*; D008279:Magnetic Resonance Imaging; D008297:Male; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D008911:Minocycline / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C100745:Aif1 protein, rat; D002135:Calcium-Binding Proteins; D005904:Glial Fibrillary Acidic Protein; D008840:Microfilament Proteins; D008911:Minocycline