title: Genotoxic and cytotoxic evaluation of pyrethroid insecticides λ-cyhalothrin and α-cypermethrin on human blood lymphocyte culture.
abstract: The present study aimed to investigate the genotoxic, cytotoxic and aneugenic effects of 1, 2, 3.75, 7.5, 15, 30 μM concentrations of the insecticides λ-cyhalothrin (LCT) and α-cypermethrin (CYP) on human peripheral blood lymphocyte culture using micronucleus (MN) and fluorescence in situ hybridisation (FISH) methods. All the concentrations were tested to assess the MN and apoptosis effects, and 1 and 2 μM LCT and 7.5 and 15 μM CYP concentrations were tested for FISH analysis. The cytotoxic effect was also observed using trypan blue and the acridine orange/ethidium bromide fluorescence staining method to measure the apoptotic effect. It was observed that both of the insecticides had a cytotoxic effect at all the concentrations (p ≤ 0.001) and apoptotic effect for LCT at 15-30 μM (p ≤ 0.05; p ≤ 0.01) for CYP between 2 and 30 μM concentrations (p ≤ 0.05; p ≤ 0.01). The micronuclei that developed after exposure were induced because of an aneugenic effect (p ≤ 0.001). LCT and CYP might be spindle poisons or caused damaged to centromere/kinetochore function.
authors.affiliation: Department of Biology, Faculty of Sciences, Trakya University, 22030, Edirne, Turkey. fulyadilek@trakya.edu.tr
authors.forename: Fulya Dilek Gokalp
authors.initials: FD
authors.lastname: Muranli
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007306:Insecticides / Q000633:toxicity*; D008214:Lymphocytes / Q000187:drug effects* / Q000473:pathology; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced*; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D009570:Nitriles / Q000633:toxicity*; D011722:Pyrethrins / Q000633:toxicity*
chemical_list: D007306:Insecticides; D009153:Mutagens; D009570:Nitriles; D011722:Pyrethrins; C017160:cypermethrin; C037304:cyhalothrin