title: Insights into scorpion venom peptides: alternative processing of β-KTx propeptide from Tityus serrulatus venom results in a new naturally occurring thimet oligopeptidase inhibitor.
abstract: Most functions attributed to Tityus serrulatus venom (TsV) are related to active molecules on ion-channels; however, here we describe a new pentapeptide that was discovered through enzymatic assay selection using EP24.15. The primary structure analysis revealed the sequence KEXXG (X means Ile or Leu), similar to the sequence present in the β-KTX propeptide described from the venom of Tityus spp. We confirmed through HPLC analysis that KEILG is the peptide present in TsV, but that KELLG also inhibits EP24.15 although through different mechanisms.
authors.affiliation: Immunochemistry Laboratory, Butantan Institute, São Paulo, Brazil.
authors.forename: Daniela Cajado
authors.initials: DC
authors.lastname: Carvalho
authors.forename: Bruno
authors.initials: B
authors.lastname: Duzzi
authors.forename: Alexandre Kazuo
authors.initials: AK
authors.lastname: Kuniyoshi
authors.forename: Mariana
authors.initials: M
authors.lastname: Fioramonte
authors.forename: Fábio Cesar
authors.initials: FC
authors.lastname: Gozzo
authors.forename: Robson L
authors.initials: RL
authors.lastname: Melo
authors.forename: Denise Vilarinho
authors.initials: DV
authors.lastname: Tambourgi
authors.forename: Vanessa
authors.initials: V
authors.lastname: Rioli
authors.forename: Fernanda Calheta
authors.initials: FC
authors.lastname: Portaro
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012604:Scorpion Venoms / Q000737:chemistry / Q000235:genetics*; D012605:Scorpions / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D010455:Peptides; D012604:Scorpion Venoms; D008666:Metalloendopeptidases; C022510:thimet oligopeptidase