title: Human papillomavirus type 38 E6 and E7 act as tumour promoters during chemically induced skin carcinogenesis.
abstract: Many findings support a possible involvement of a subgroup of human papillomaviruses (HPVs), called cutaneous beta HPV types, in the development of non-melanoma skin cancer. The skin of transgenic (Tg) mice expressing viral oncoproteins E6 and E7 from different cutaneous beta HPV types, including HPV38, showed an increased susceptibility to UV-induced and/or chemically induced skin carcinogenesis compared with wild-type animals. In this study, we show that beta HPV38 E6 and E7 oncoproteins act as promoter and progression factors in multi-stage skin carcinogenesis, strongly cooperating with the initiator and DNA damage agent 7,12-dimethylbenz[a]anthracene. In contrast, exposure of HPV38 E6/E7 Tg mice to the promoter 12-O-tetradecanoylphorbol-13-acetate did not significantly result in the development of skin lesions. These findings further support the role of beta HPV types in skin carcinogenesis, providing additional insight into their precise contribution to the multi-step process.
authors.affiliation: German Cancer Research Center (DKFZ), Heidelberg, Germany.
authors.forename: Daniele
authors.initials: D
authors.lastname: Viarisio
authors.affiliation: German Cancer Research Center (DKFZ), Heidelberg, Germany.
authors.forename: Karin Müller
authors.initials: KM
authors.lastname: Decker
authors.affiliation: German Cancer Research Center (DKFZ), Heidelberg, Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Aengeneyndt
authors.affiliation: Department of Pathology, University Hospital of Heidelberg, Heidelberg, Germany.
authors.forename: Christa
authors.initials: C
authors.lastname: Flechtenmacher
authors.affiliation: Department of Botany and Microbiology (honorary member), King Saud University, Riyadh, Saudi Arabia.|German Cancer Research Center (DKFZ), Heidelberg, Germany.
authors.forename: Lutz
authors.initials: L
authors.lastname: Gissmann
authors.affiliation: International Agency for Research on Cancer, World Health Organization, Lyon, France.
authors.forename: Massimo
authors.initials: M
authors.lastname: Tommasino
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000378:metabolism*; D000818:Animals; D002273:Carcinogens / Q000378:metabolism*; D005260:Female; D051379:Mice; D008822:Mice, Transgenic; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D027383:Papillomaviridae / Q000472:pathogenicity*; D012867:Skin / Q000187:drug effects* / Q000528:radiation effects; D012878:Skin Neoplasms / Q000139:chemically induced / Q000821:virology
chemical_list: D002273:Carcinogens; D009856:Oncogene Proteins, Viral; D015127:9,10-Dimethyl-1,2-benzanthracene