title: A New Standard-Based Polynomial Interpolation (SBPIn) method to address gel-to-gel variability for the comparison of multiple denaturing gradient gel electrophoresis profile matrices.
abstract: The Standard-Based Polynomial Interpolation (SBPIn) method is a new simple three-step protocol proposed to address common gel-to-gel variations for the comparison of sample profiles across multiple DGGE gels. The advantages of this method include no requirement for additional software or modification of the standard DGGE protocol.
authors.affiliation: Department of Soil, Water and Environmental Science, 429 Shantz Bldg. #38, 1177 E. Fourth Street, University of Arizona, Tucson, Arizona 85721-0038, USA. valentin@email.arizona.edu
authors.forename: Alexis
authors.initials: A
authors.lastname: Valentín-Vargas
authors.forename: Jon
authors.initials: J
authors.lastname: Chorover
authors.forename: Raina M
authors.initials: RM
authors.lastname: Maier
mesh_terms: D003627:Data Interpretation, Statistical; D058645:Denaturing Gradient Gel Electrophoresis / Q000379:methods* / Q000592:standards*; D015203:Reproducibility of Results