title: Optimization of the selective preparation of 20(R)-ginsenoside Rg3 catalyzed by d, l-tartaric acid using response surface methodology.
abstract: The optimization of the selective preparation of 20(R)-ginsenoside Rg3 converting protopanaxadiol type saponins (PPD saponins) by the commercially available d, l-tartaric acid was carried out using response surface methodology (RSM) based on a three-factor and six-level central composite design. The optimal 20(R)-ginsenoside Rg3 de% was predicted to be 94.52% in the combination of the factors (d, l-tartaric acid concentration 1.19mol/L, temperature 107.9°C and time 2.79h) through the canonical analysis with maximum responses. Under the optimum reaction conditions, the actual 20(R)-ginsenoside Rg3 de% was 96.49%. 20(R)-ginsenoside Rg3 (1) and 20(S)-ginsenoside Rg3 (2) were separated and identified by (1)H-NMR and (13)C-NMR. Therefore, the RSM was effective to optimize the preparation of 20(R)-ginsenoside Rg3 by converting PPD saponins using d, l-tartaric acid.
authors.affiliation: Department of Chemistry, Northeast Normal University, Changchun, China.
authors.forename: Chengpeng
authors.initials: C
authors.lastname: Sun
authors.forename: Weiping
authors.initials: W
authors.lastname: Gao
authors.forename: Baozhong
authors.initials: B
authors.lastname: Zhao
authors.forename: Leqin
authors.initials: L
authors.lastname: Cheng
mesh_terms: D002384:Catalysis; D036145:Ginsenosides / Q000138:chemical synthesis*; D008956:Models, Chemical; D015394:Molecular Structure; D013644:Tartrates / Q000737:chemistry*
chemical_list: D036145:Ginsenosides; D013644:Tartrates; C097367:ginsenoside Rg3; C029768:tartaric acid