title: Chromatin-dependent and -independent regulation of DNA replication origin activation in budding yeast.
abstract: To elucidate the role of the chromatin environment in the regulation of replication origin activation, autonomously replicating sequences were inserted into identical locations in the budding yeast genome and their activation times in S phase determined. Chromatin-dependent origins adopt to the firing time of the surrounding locus. In contrast, the origins containing two binding sites for Forkhead transcription factors are activated early in the S phase regardless of their location in the genome. Our results also show that genuinely late-replicating parts of the genome can be converted into early-replicating loci by insertion of a chromatin-independent early replication origin, ARS607, whereas insertion of two Forkhead-binding sites is not sufficient for conversion.
authors.affiliation: Institute of Molecular and Cell Biology, University of Tartu, Riia 23, Tartu 51010, Estonia.
authors.forename: Marko
authors.initials: M
authors.lastname: Lõoke
authors.forename: Kersti
authors.initials: K
authors.lastname: Kristjuhan
authors.forename: Signe
authors.initials: S
authors.lastname: Värv
authors.forename: Arnold
authors.initials: A
authors.lastname: Kristjuhan
mesh_terms: D001483:Base Sequence; D002843:Chromatin / Q000502:physiology*; D004261:DNA Replication; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding; D018741:Replication Origin; D016196:S Phase; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
chemical_list: C102209:CDC45 protein, S cerevisiae; D002843:Chromatin; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins