title: Bone apposition to laminin-1 coated implants: histologic and 3D evaluation.
abstract: Laminin-1 has been reported as one of the factors responsible for the nucleation of calcium phosphates and, in vitro, has been reported to selectively recruit osteoprogenitors. This article focused on its in vivo effects, and evaluated the effect of laminin-1 local application on osseointegration. Polished cylindrical hydroxyapatite implants were coated with laminin-1 (test) and the bone responses in the rabbit tibiae after 2 and 4 weeks were evaluated and compared to the non-coated implants (control). Before the samples were processed for histological sectioning, they were three-dimensionally analysed with micro computed tomography (μCT). Both evaluation methods were analysed with regards to bone area around the implant and bone to implant contact. From the histologic observation, new bone formation around the laminin-1 coated implant at 2 weeks seemed to have increased the amount of supporting bone around the implant, however, at 4 weeks, the two groups presented no notable differences. The two-dimensional and three-dimensional morphometric evaluation revealed that both histologic and three-dimensional analysis showed some tendency in favour of the test group implants, however there was no statistical significance between the test and control group results.
authors.affiliation: Department of Prosthodontics, Faculty of Odontology, Malmö University, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Bougas
authors.forename: R
authors.initials: R
authors.lastname: Jimbo
authors.forename: S
authors.initials: S
authors.lastname: Vandeweghe
authors.forename: M
authors.initials: M
authors.lastname: Hayashi
authors.forename: M
authors.initials: M
authors.lastname: Bryington
authors.forename: Y
authors.initials: Y
authors.lastname: Kozai
authors.forename: H O
authors.initials: HO
authors.lastname: Schwartz-Filho
authors.forename: N
authors.initials: N
authors.lastname: Tovar
authors.forename: E
authors.initials: E
authors.lastname: Adolfsson
authors.forename: D
authors.initials: D
authors.lastname: Ono
authors.forename: P G
authors.initials: PG
authors.lastname: Coelho
authors.forename: A
authors.initials: A
authors.lastname: Wennerberg
mesh_terms: D000327:Adsorption; D000818:Animals; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D017076:Computer-Aided Design; D045662:Dental Etching / Q000379:methods; D015921:Dental Implants; D018130:Diamond / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry; D007091:Image Processing, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods; D007368:Interferometry / Q000379:methods; D007797:Laminin / Q000494:pharmacology*; D008297:Male; D016348:Osseointegration / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects; D011817:Rabbits; D013499:Surface Properties; D013977:Tibia / Q000187:drug effects / Q000473:pathology; D013997:Time Factors; D055114:X-Ray Microtomography / Q000379:methods
chemical_list: D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D007797:Laminin; C121346:laminin 1; D018130:Diamond; D017886:Durapatite