title: Evaluation of single nucleotide polymorphisms in microRNAs (hsa-miR-196a2 rs11614913 C/T) from Brazilian women with breast cancer.
abstract: BACKGROUND
  Emerging evidence has shown that miRNAs are involved in human carcinogenesis as tumor suppressors or oncogenes. Single nucleotide polymorphisms (SNPs) located in pre-miRNAs may affect the processing and therefore, influence the expression of mature miRNAs. Previous studies generated conflicting results when reporting association between the hsa-miR-196a2 rs11614913 common polymorphism and breast cancer.
  
  
  METHODS
  This study evaluated the hsa-miR-196a2 rs11614913 SNP in 388 breast cancer cases and 388 controls in Brazilian women. Polymorphism was determined by real-time PCR; control and experimental groups were compared through statistical analysis using the X2 or Fisher's exact tests.
  
  
  RESULTS
  The analysis of the SNPs frequencies showed a significant difference between the groups (BC and CT) in regards to genotype distribution (χ2: p = 0.024); the homozygous variant (CC) was more frequent in the CT than in the BC group (p = 0.009). The presence of the hsa-miR-196a2 rs11614913 C/T polymorphism was not associated with histological grades (p = 0.522), axillary lymph node positive status (p = 0.805), or clinical stage (p = 0.670) among the breast cancer patients.
  
  
  CONCLUSIONS
  The results of this study indicated that the CC polymorphic genotype is associated with a decreased risk of BC and the presence of the T allele was significantly associated with an increased risk of BC.
authors.affiliation: Department of Gynecology and Obstetrics, Federal University of Ceará (Campus Sobral), Av, Humberto Lopes, 200, Junco, Sobral, Ceará, CEP: 62022-304, Brazil. juvenalinhares@gmail.com
authors.forename: José Juvenal
authors.initials: JJ
authors.lastname: Linhares
authors.forename: Marcos
authors.initials: M
authors.lastname: Azevedo
authors.forename: Adalberto Abraão
authors.initials: AA
authors.lastname: Siufi
authors.forename: Cristina Valleta
authors.initials: CV
authors.lastname: de Carvalho
authors.forename: Maria Del Carmen Garcia Molina
authors.initials: Mdel C
authors.lastname: Wolgien
authors.forename: Emmanuelle Coelho
authors.initials: EC
authors.lastname: Noronha
authors.forename: Tatiana Carvalho de Souza
authors.initials: TC
authors.lastname: Bonetti
authors.forename: Ismael Dale Cotrim Guerreiro
authors.initials: ID
authors.lastname: da Silva
mesh_terms: D000368:Aged; D000483:Alleles; D001938:Brazil; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: C542543:MIRN196 microRNA, human; D035683:MicroRNAs