title: Resolution of a paradox by native mass spectrometry: facile occupation of all four metal binding sites in the dimeric zinc sensor SmtB.
abstract: The predominant species of the cyanobacterial metalloregulatory protein SmtB as observed by ESI-MS is a dimer with all four zinc binding sites occupied.
authors.affiliation: School of Life Sciences, The University of Warwick, Coventry, UK. f.d.l.kondrat@warwick.ac.uk
authors.forename: Frances D L
authors.initials: FD
authors.lastname: Kondrat
authors.forename: Gregory R
authors.initials: GR
authors.lastname: Kowald
authors.forename: Charlotte A
authors.initials: CA
authors.lastname: Scarff
authors.forename: James H
authors.initials: JH
authors.lastname: Scrivens
authors.forename: Claudia A
authors.initials: CA
authors.lastname: Blindauer
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D008958:Models, Molecular; D055503:Protein Multimerization; D021241:Spectrometry, Mass, Electrospray Ionization; D046940:Synechococcus / Q000737:chemistry* / Q000378:metabolism; D015032:Zinc / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D015032:Zinc