title: Mating type gene (MAT1-1) in Japanese isolates of Trichophyton rubrum.
abstract: Trichophyton rubrum is an anthropophilic species that is the most frequent etiologic agent of human dermatophytosis throughout the world. No teleomorph has been identified for T. rubrum strains. This study used PCR analysis to confirm the presence of a mating type locus in the genome of Japanese isolates of T. rubrum. To clarify the epidemiological and ecological characteristics of this fungus, mating type sequences were tested for correlation of MAT genotype to mating type. This study examined clinical isolates of T. rubrum that had been obtained from 206 human cases of tinea pedis and tinea unguium in Japan, including those from Fukuoka (29 strains), Gifu (23 strains), Kanazawa (63 strains), and Tokyo (91 strains), along with 10 isolates derived from 10 cases of canine dermatophytosis. PCR detected the presence of MAT1-1 in all of the human and animal isolates. Therefore, all isolates examined were expected to react as (-) type on the mating test and not as (+) type.
authors.affiliation: Department of Pathobiology, Nihon University School of Veterinary Medicine, 1866, Fujisawa, Kanagawa, 252-0880, Japan. kano@brs.nihon-u.ac.jp
authors.forename: Rui
authors.initials: R
authors.lastname: Kano
authors.forename: Maiko
authors.initials: M
authors.lastname: Isizuka
authors.forename: Masataro
authors.initials: M
authors.lastname: Hiruma
authors.forename: Takashi
authors.initials: T
authors.lastname: Mochizuki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kamata
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Hasegawa
mesh_terms: D000818:Animals; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004285:Dogs; D049770:Genes, Mating Type, Fungal; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008969:Molecular Sequence Data; D058889:Molecular Typing; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014005:Tinea / Q000453:epidemiology / Q000382:microbiology* / Q000662:veterinary; D014249:Trichophyton / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004271:DNA, Fungal