title: Rapid and sensitive detection of Acinetobacter baumannii using loop-mediated isothermal amplification.
abstract: Here we report the design and evaluation of a loop-mediated isothermal amplification (LAMP) assay for detecting Acinetobacter baumannii DNA based on the 16S-23S rRNA intergenic spacer (ITS) sequence. The results showed that target DNA was amplified and visualized within 30min and with a detection limit 100-fold greater than PCR.
authors.affiliation: Department of Laboratory Medicine and Biotechnology, Tzu Chi University, Hualien, Taiwan.
authors.forename: Po-Chi
authors.initials: PC
authors.lastname: Soo
authors.forename: Chun-Chieh
authors.initials: CC
authors.lastname: Tseng
authors.forename: Siao-Ru
authors.initials: SR
authors.lastname: Ling
authors.forename: Ming-Li
authors.initials: ML
authors.lastname: Liou
authors.forename: Chih-Chin
authors.initials: CC
authors.lastname: Liu
authors.forename: Huei-Jen
authors.initials: HJ
authors.lastname: Chao
authors.forename: Teng-Yi
authors.initials: TY
authors.lastname: Lin
authors.forename: Kai-Chih
authors.initials: KC
authors.lastname: Chang
mesh_terms: D000151:Acinetobacter Infections / Q000175:diagnosis; D040981:Acinetobacter baumannii / Q000235:genetics / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer