title: A combination approach for rapid and high yielding purification of bacterial minicells.
abstract: A method for bacterial minicell purification was developed by combining antibiotic (ceftriaxone) lysis and filtration. This method is fast, cost effective and facilitates high yield of purified minicells, with no parent strain contamination as confirmed by fluorescent microscopy, average particle size and polydispersity index.
authors.affiliation: Department of Pharmacology and Toxicology, B. V. Patel Pharmaceutical Education and Research Development (PERD) Centre, Sarkhej-Gandhinagar Highway, Thaltej, Ahmedabad, 380 054 Gujarat, India.
authors.forename: Mehul
authors.initials: M
authors.lastname: Jivrajani
authors.forename: Neeta
authors.initials: N
authors.lastname: Shrivastava
authors.forename: Manish
authors.initials: M
authors.lastname: Nivsarkar
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism; D001419:Bacteria / Q000187:drug effects / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D001433:Bacteriolysis; D002443:Ceftriaxone / Q000378:metabolism; D005374:Filtration / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D002443:Ceftriaxone