title: [Nestin and Musashi1 as the markers of neural stem cells in rat telencephalon following transitory focal ischemia].
abstract: Nestin and Musashil (Msi-1) proteins are most often used for labeling of neural stem cells and progenitor cells in vivo, however it remains unclear if these markers really label the same cells. As a result of the study of structural characteristics and localization of nestin- and Msil-expressing cells it was found that these proteins were detected in non-identical cell populations in the brain of intact 15 rats. We failed to find cell groups demonstrating a coexpression of nestin and Msi-1. However, after ischemic lesion of the brain, which was caused in 38 rats by an endovascular occlusion of the left medial cerebral artery for 30 min with the following reperfusion for 48 hours, both markers were detected in cells of subventricular zone and in ependymocytes. These results indicate the changes in cytochemical patterns of the candidate stem cells.
authors.forename: O V
authors.initials: OV
authors.lastname: Kirik
authors.forename: T D
authors.initials: TD
authors.lastname: Vlasov
authors.forename: D É
authors.initials: DÉ
authors.lastname: Korzhevskiĭ
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis*; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation; D007381:Intermediate Filament Proteins / Q000096:biosynthesis*; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D064231:Nestin; D058953:Neural Stem Cells / Q000378:metabolism* / Q000473:pathology; D016601:RNA-Binding Proteins / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D013687:Telencephalon / Q000378:metabolism* / Q000473:pathology
chemical_list: D000943:Antigens, Differentiation; D007381:Intermediate Filament Proteins; C491296:Msi1 protein, rat; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D016601:RNA-Binding Proteins