title: Vinculin-dependent Cadherin mechanosensing regulates efficient epithelial barrier formation.
abstract: Proper regulation of the formation and stabilization of epithelial cell-cell adhesion is crucial in embryonic morphogenesis and tissue repair processes. Defects in this process lead to organ malformation and defective epithelial barrier function. A combination of chemical and mechanical cues is used by cells to drive this process. We have investigated the role of the actomyosin cytoskeleton and its connection to cell-cell junction complexes in the formation of an epithelial barrier in MDCK cells. We find that the E-cadherin complex is sufficient to mediate a functional link between cell-cell contacts and the actomyosin cytoskeleton. This link involves the actin binding capacity of α-catenin and the recruitment of the mechanosensitive protein Vinculin to tensile, punctate cell-cell junctions that connect to radial F-actin bundles, which we name Focal Adherens Junctions (FAJ). When cell-cell adhesions mature, these FAJs disappear and linear junctions are formed that do not contain Vinculin. The rapid phase of barrier establishment (as measured by Trans Epithelial Electrical Resistance (TER)) correlates with the presence of FAJs. Moreover, the rate of barrier establishment is delayed when actomyosin contraction is blocked or when Vinculin recruitment to the Cadherin complex is prevented. Enhanced presence of Vinculin increases the rate of barrier formation. We conclude that E-cadherin-based FAJs connect forming cell-cell adhesions to the contractile actomyosin cytoskeleton. These specialized junctions are sites of Cadherin mechanosensing, which, through the recruitment of Vinculin, is a driving force in epithelial barrier formation.
authors.affiliation: Hubrecht Institute for Developmental Biology and Stem Cell Research and University Medical Centre Utrecht ,  PO Box 85164, 3508 AD Utrecht ,  The Netherlands.
authors.forename: Floor
authors.initials: F
authors.lastname: Twiss
authors.forename: Quint
authors.initials: Q
authors.lastname: Le Duc
authors.forename: Suzanne
authors.initials: S
authors.lastname: Van Der Horst
authors.forename: Hamid
authors.initials: H
authors.lastname: Tabdili
authors.forename: Gerard
authors.initials: G
authors.lastname: Van Der Krogt
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Holger
authors.initials: H
authors.lastname: Rehmann
authors.forename: Stephan
authors.initials: S
authors.lastname: Huveneers
authors.forename: Deborah E
authors.initials: DE
authors.lastname: Leckband
authors.forename: Johan
authors.initials: J
authors.lastname: De Rooij