title: Dual nano-electrospray for probing solution interactions and fast reactions of complex biomolecules.
abstract: A novel nano-electrospray emitter has been developed containing two separated channels running throughout the length of the emitter. The emitters have been fabricated from  "theta-shaped" borosilicate capillaries. Loading of different solutions into the two different channels opens up the possibility to study short timescale interactions within a Taylor cone common to both channels. The common Taylor cone constitutes an extremely small "mixing volume" of the order of femtolitres. The products of electrospray from the dual-channel emitters have been analysed by Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry. Results are presented for interactions of vancomycin with diacetyl-L-lysyl-D-alanyl- D-alanine and interactions of vancomycin with deuterated vancomycin. On the basis of these results, it is concluded that, during electrospray, specific non-covalent adducts have been formed and that there have been  exchange reactions involving making and breaking of covalent bonds.
authors.affiliation: Department of Chemistry, University of Warwick, Coventry, UK.
authors.forename: Lewis P
authors.initials: LP
authors.lastname: Mark
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Gill
authors.forename: Marek
authors.initials: M
authors.lastname: Mahut
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Derrick
mesh_terms: D003903:Deuterium; D004867:Equipment Design; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods*; D009842:Oligopeptides / Q000737:chemistry*; D012996:Solutions; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D014640:Vancomycin / Q000737:chemistry*
chemical_list: D009842:Oligopeptides; D012996:Solutions; C033530:N(alpha), N-(epsilon)-diacetyl-lysyl-alanyl-alanine; D014640:Vancomycin; D003903:Deuterium