title: Usefulness of capillary electrophoresis-based multiplex PCR assay for species-specific identification of Candida spp.
abstract: The study evaluated the performances of a commercial multiplex PCR assay, the Seegene Seeplex STI Master Panel 3, for Candida spp. identification. Eighty clinical strains of Candida spp. were identified with this system and a homemade multiplex PCR assay. The results were also compared with those obtained with two phenotypic methods. The study provided a preliminary evaluation of a multiplex assay from Seegene that uses capillary electrophoresis as the detection of amplified products. The Seeplex assay was found to be a rapid and useful method for identifying large numbers of yeast isolates in the clinical laboratory context.
authors.affiliation: Department of Biomedical Sciences, Microbiology and Virology Unit, University of Cagliari, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Mallus
authors.forename: S
authors.initials: S
authors.lastname: Martis
authors.forename: C
authors.initials: C
authors.lastname: Serra
authors.forename: G
authors.initials: G
authors.lastname: Loi
authors.forename: T
authors.initials: T
authors.lastname: Camboni
authors.forename: A
authors.initials: A
authors.lastname: Manzin
mesh_terms: D002175:Candida / Q000145:classification* / Q000235:genetics*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D009172:Mycology / Q000379:methods*; D013997:Time Factors