title: Mosaic acquisition and processing for optical-resolution photoacoustic microscopy.
abstract: In optical-resolution photo-acoustic microscopy (OR-PAM), data acquisition time is limited by both laser pulse repetition rate (PRR) and scanning speed. Optical-scanning offers high speed, but limited, field of view determined by ultrasound transducer sensitivity. In this paper, we propose a hybrid optical and mechanical-scanning OR-PAM system with mosaic data acquisition and processing. The system employs fast-scanning mirrors and a diode-pumped, nanosecond-pulsed, Ytterbium-doped, 532-nm fiber laser with PRR up to 600 kHz. Data from a sequence of image mosaic patches is acquired systematically, at predetermined mechanical scanning locations, with optical scanning. After all imaging locations are covered, a large panoramic scene is generated by stitching the mosaic patches together. Our proposed system is proven to be at least 20 times faster than previous reported OR-PAM systems.
authors.affiliation: University of Alberta, Department of Electrical & Computer Engineering, Edmonton T6G2V4, Canada.
authors.forename: Peng
authors.initials: P
authors.lastname: Shao
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.forename: Ryan K W
authors.initials: RK
authors.lastname: Chee
authors.forename: Roger J
authors.initials: RJ
authors.lastname: Zemp
mesh_terms: D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D053844:Lasers, Solid-State; D018613:Microscopy, Confocal / Q000295:instrumentation*; D061088:Photoacoustic Techniques / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013382:Subtraction Technique / Q000295:instrumentation*