title: Serum neutrophil gelatinase-associated lipocalin as a marker of acute kidney injury in asphyxiated neonates.
abstract: OBJECTIVE
  To determine the clinical utility of serum neutrophil gelatinase-associated lipocalin (NGAL) as an early marker of acute kidney injury in asphyxiated neonates with hypoxic ischemic encephalopathy (HIE).
  
  
  METHODS
  Cohort study.
  
  
  METHODS
  National Intensive Care Unit of Maternity Hospital, Ain Shams University, Cairo, Egypt.
  
  
  METHODS
  The study included 30 term asphyxiated neonates (8 with mild, 13 with moderate and 9 with severe HIE) and 20 control neonates.
  
  
  METHODS
  Serum NGAL level was measured within 6 hours after birth using an enzyme linked immunosorbent assay.
  
  
  METHODS
  Patients were subsequently discriminated into AKI (n=12) and no-AKI (n=18) groups.
  
  
  RESULTS
  The median (Interquartile range) serum NGAL concentration was 95.0 (70.75-180.00) ng/mL in asphyxiated neonates, and 39.75 (6.0-48.0) ng/mL in control neonates; (P<0.001). Serum NGAL correlated with HIE severity: mean (SD) was 65.50 (3.77) ng/mL in infants with mild HIE, 115.07 (45.83) ng/mL in infants with moderate HIE and 229.66 (79.50) ng/mL in infants with severe HIE; (P<0.01). The median (Interquartiles) serum NGAL level was 182.50 (166.25-301.75) ng/mL in patients with AKI, 74.00 (66.00-78.75) ng/mL in those without AKI; (P<0.001). A cutoff value 157 ng/mL for serum NGAL could detect AKI in asphyxiated neonates with a sensitivity of 83.3% and a specificity of 94.4%.
  
  
  CONCLUSIONS
  Elevated serum NGAL measured within 6 hours after birth reliably indicates acute kidney injury in asphyxiated neonates.
authors.affiliation: Departments of Pediatrics, and *Clinical Pathology, Faculty of Medicine - Ain Shams University, Egypt. soha_khafagy@yahoo.com
authors.forename: Nm El
authors.initials: NE
authors.lastname: Raggal
authors.forename: S M
authors.initials: SM
authors.lastname: Khafagy
authors.forename: N H
authors.initials: NH
authors.lastname: Mahmoud
authors.forename: Sa El
authors.initials: SE
authors.lastname: Beltagy
mesh_terms: D058186:Acute Kidney Injury / Q000097:blood*; D000209:Acute-Phase Proteins; D001238:Asphyxia Neonatorum / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D015331:Cohort Studies; D005260:Female; D006801:Humans; D020925:Hypoxia-Ischemia, Brain / Q000097:blood; D007231:Infant, Newborn; D000071068:Lipocalin-2; D054834:Lipocalins / Q000097:blood*; D008297:Male; D011518:Proto-Oncogene Proteins / Q000097:blood*; D016896:Treatment Outcome
chemical_list: D000209:Acute-Phase Proteins; D015415:Biomarkers; C489506:LCN2 protein, human; D000071068:Lipocalin-2; D054834:Lipocalins; D011518:Proto-Oncogene Proteins