title: [Molecular characterization of the multidrug-resistant Acinetobacter baumannii strains and assessment of their sensitivity to the phage AP22].
abstract: The molecular analysis of 130 multidrug-resistant nosocomial Acinetobacter baumannii strains was performed. The strains were obtained from patients admitted to different Russian hospitals (Chelyabinsk, Moscow, Nizhni Novgorod, and St. Petersburg) in 2005-2010. Species identification was performed using the amplified 16S rRNA gene restriction analysis and by determining intrinsic for A. baumannii blaQXA-51-like genes using PCR. The genetic typing of the strains was performed by RAPD-PCR. All strains fell into two clusters: A and B with dominant RAPD-groups A1 and B1, respectively, including 82% (107 of 130) of all studied strains. The susceptibility to the bacteriophage AP22 of the strains was determined. The phage was found to infect specifically and to constitute 69% of 130 strains and 82% (88 of 107) of the A. baumannii strains from the dominant RAPD groups. The ability of the bacteriophage AP22 to constitute a broad range of the clinically relevant A. baumannii strains makes it an attractive candidate for designing the phage cocktails intended to control the A. baumannii-associated nosocomial infections. Moreover, the phage can be used for the identification of A. baumannii in bacteriological analysis of clinical materials.
authors.forename: A V
authors.initials: AV
authors.lastname: Popova
authors.forename: V P
authors.initials: VP
authors.lastname: Miakinina
authors.forename: M E
authors.initials: ME
authors.lastname: Platonov
authors.forename: N V
authors.initials: NV
authors.lastname: Volozhantsev
mesh_terms: D040981:Acinetobacter baumannii / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D001435:Bacteriophages / Q000235:genetics / Q000472:pathogenicity*; D003428:Cross Infection / Q000235:genetics / Q000382:microbiology*; D018432:Drug Resistance, Multiple / Q000235:genetics; D005838:Genotype; D006801:Humans; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
chemical_list: D012336:RNA, Ribosomal, 16S