title: Quantitative PCR confirms culture as the gold standard for detection of lower airway infection by nontypeable Haemophilus influenzae in Australian Indigenous children with bronchiectasis.
abstract: Correlation was observed between quantitative PCR and semi-quantitative culture for definition of Haemophilus influenzae infection in bronchoalveolar lavage specimens from 81 children with bronchiectasis. However, qPCR data correlated less well with airway neutrophilia, and supports continued use of culture as the gold standard for defining H. influenzae lower airway infection.
authors.affiliation: Child Health Division, Menzies School of Health Research, Charles Darwin University, PO Box 41096, Casuarina, Darwin, Northern Territory 0811, Australia. kim.hare@menzies.edu.au
authors.forename: Kim M
authors.initials: KM
authors.lastname: Hare
authors.forename: Robyn L
authors.initials: RL
authors.lastname: Marsh
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Binks
authors.forename: Keith
authors.initials: K
authors.lastname: Grimwood
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Pizzutto
authors.forename: Amanda J
authors.initials: AJ
authors.lastname: Leach
authors.forename: Anne B
authors.initials: AB
authors.lastname: Chang
authors.forename: Heidi C
authors.initials: HC
authors.lastname: Smith-Vaughan
mesh_terms: D001315:Australia; D001431:Bacteriological Techniques / Q000379:methods*; D001987:Bronchiectasis / Q000150:complications*; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D001996:Bronchopneumonia / Q000175:diagnosis* / Q000382:microbiology; D002648:Child; D002675:Child, Preschool; D005260:Female; D006192:Haemophilus Infections / Q000175:diagnosis* / Q000382:microbiology; D006193:Haemophilus influenzae / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D044382:Population Groups; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*