title: Effect of myeloperoxidase and anoxia/reoxygenation on mitochondrial respiratory function of cultured primary equine skeletal myoblasts.
abstract: Horses are particularly sensitive to excessive inflammatory reaction where myeloperoxidase, a marker of inflammation, may contribute to mitochondrial dysfunctions. This study investigated the interaction between myeloperoxidase and cultured primary equine skeletal myoblasts, particularly its effect on mitochondrial respiration combined or not with anoxia followed by reoxygenation (AR). We showed that active myeloperoxidase entered into the cells, interacted with mitochondria and decreased routine and maximal respirations. When combined with AR, myeloperoxidase caused a further decrease of these respiratory parameters while the leak increased. Our results indicate that myeloperoxidase amplifies the mitochondrial damages initiated by AR phenomenon and alters the mitochondrial function.
authors.affiliation: Center for Oxygen Research and Development, Institute of Chemistry B6a, University of Liège, Sart Tilman, 4000 Liège, Belgium. j.ceusters@ulg.ac.be
authors.forename: Justine D
authors.initials: JD
authors.lastname: Ceusters
authors.forename: Ange A
authors.initials: AA
authors.lastname: Mouithys-Mickalad
authors.forename: Thierry J
authors.initials: TJ
authors.lastname: Franck
authors.forename: Sandrine
authors.initials: S
authors.lastname: Derochette
authors.forename: Alain
authors.initials: A
authors.lastname: Vanderplasschen
authors.forename: Ginette P
authors.initials: GP
authors.lastname: Deby-Dupont
authors.forename: Didier A
authors.initials: DA
authors.lastname: Serteyn
mesh_terms: D000818:Animals; D019069:Cell Respiration; D002478:Cells, Cultured; D006736:Horses; D000860:Hypoxia; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism*; D032448:Myoblasts, Skeletal / Q000502:physiology*; D010100:Oxygen / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism*
chemical_list: D009195:Peroxidase; D010100:Oxygen