title: [Anti-Mur screening and Mur antigen genotyping of voluntary blood donors in Guangzhou].
abstract: OBJECTIVE
  To investigate the frequency of anti-Mur and Mur antigen among blood donors in Guangzhou to provide evidence for guiding clinical transfusion and prenatal screening.
  
  
  METHODS
  DG Gel Coombs cards were used to screen active anti-Mur at 37 degrees celsius; from 2725 blood donors. Multiplex ligation-dependent probe amplification (MLPA) was used to genotype Mur antigen from 91 blood donors, and human anti-Mur serum was used to verify the phenotypes deduced from the genotypes.
  
  
  RESULTS
  The frequency of anti-Mur and genotyped Mur antigen was 0.04% (1/2725) and 6.59% (6/91), respectively, and the phenotyping results were consistent with the genotyping results.
  
  
  CONCLUSIONS
  The blood donors in Guangzhou show a low frequency of anti-Mur and a relatively high frequency of Mur antigen. Genotyping using MLPA allows Mur antigen genotyping when commercial anti-Mur is not available.
authors.affiliation: Institute of Clinical Blood Transfusion, Guangzhou Blood Center, Guangzhou 510095, China. wlmingyue@163.com
authors.forename: Ling
authors.initials: L
authors.lastname: Wei
authors.forename: Yanli
authors.initials: Y
authors.lastname: Ji
authors.forename: Chunyan
authors.initials: C
authors.lastname: Mo
authors.forename: Runqing
authors.initials: R
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Hong
authors.initials: H
authors.lastname: Luo
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Guangping
authors.initials: G
authors.lastname: Luo
mesh_terms: D001782:Blood Donors; D001789:Blood Group Antigens / Q000235:genetics* / Q000276:immunology*; D002681:China; D005838:Genotype; D060005:Genotyping Techniques; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction; D010641:Phenotype
chemical_list: D001789:Blood Group Antigens