title: Laser-printing of toner-based 96-microzone plates for immunoassays.
abstract: This work describes the quick and simple fabrication of toner-based 96-microzone plates by a direct-printing technology. The printer deposits a toner layer (ca. 5 μm thick) on the polyester surface which acts as a hydrophobic barrier to confine small volumes of sample on test zones (wells). A 96-microzone toner plate was explored to demonstrate its capability of performing enzyme-linked immunosorbent assay (ELISA). The detection of anti-immunoglobulin G (anti-IgG) and immunoglobulin M (IgM) antibodies has been successfully achieved in cell culture and serum samples, respectively. The use of a conventional microplate reader has allowed obtaining a limit of detection of 13 fmol of mouse IgG per zone on printed microplates. The IgM antibody has been detected in a serum sample collected from a patient infected with dengue virus. The detection of a primary infection has been provided by a microplate reader and also by a cell phone camera. Besides the bioanalytical feasibility, toner-based zones have shown good repeatability for inter-zone and intra-plate comparisons. The relative standard deviation (RSD) values for inter-zone (n = 12) and intra-plate (n = 3) comparisons were lower than 6% and 11%, respectively. Furthermore, it was found that the lifetime of each printed microplate depends on the storage temperature. The shelf life for devices stored at 10 °C has been estimated to be ca. four weeks.
authors.affiliation: Instituto de Química, Universidade Federal de Goiás, Campus Samambaia, 74001-970, Goiânia, GO, Brazil.
authors.forename: Karoliny Almeida
authors.initials: KA
authors.lastname: Oliveira
authors.forename: Cristina
authors.initials: C
authors.lastname: Rodrigues de Oliveira
authors.forename: Lucimeire
authors.initials: L
authors.lastname: Antonelli da Silveira
authors.forename: Wendell Karlos Tomazelli
authors.initials: WK
authors.lastname: Coltro
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007834:Lasers / Q000706:statistics & numerical data; D051379:Mice; D011327:Printing / Q000379:methods*