title: Wogonin inhibits H2O2-induced vascular permeability through suppressing the phosphorylation of caveolin-1.
abstract: Wogonin, a naturally occurring monoflavonoid extracted from the root of Scutellaria baicalensis Georgi, has been reported for its anti-oxidant activity. However, it is still unclear whether wogonin can inhibit oxidant-induced vascular permeability. In this study, we evaluated the effects of wogonin on H2O2-induced vascular permeability in human umbilical vein endothelial cells (HUVECs). We found that wogonin can suppress the H2O2-stimulated actin remodeling and albumin uptake of HUVECs, as well as transendothelial cell migration of the human breast carcinoma cell MDA-MB-231. The mechanism revealed that wogonin inhibited H2O2-induced phosphorylation of caveolin-1 (cav-1) associating with the suppression of stabilization of VE-cadherin and β-catenin. Moreover, wogonin repressed anisomycin-induced phosphorylation of p38, cav-1 and vascular permeability. These results suggested that wogonin could inhibit H2O2-induced vascular permeability by downregulating the phosphorylation of cav-1, and that it might have a therapeutic potential for the diseases associated with the development of both oxidant and vascular permeability.
authors.affiliation: State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.forename: Xiuming
authors.initials: X
authors.lastname: Song
authors.forename: Mi
authors.initials: M
authors.lastname: Zhou
authors.forename: Libin
authors.initials: L
authors.lastname: Wei
authors.forename: Qinsheng
authors.initials: Q
authors.lastname: Dai
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Li
authors.forename: Na
authors.initials: N
authors.lastname: Lu
authors.forename: Qinglong
authors.initials: Q
authors.lastname: Guo
mesh_terms: D000199:Actins / Q000378:metabolism; D000418:Albumins / Q000378:metabolism; D015153:Blotting, Western; D002199:Capillary Permeability / Q000187:drug effects*; D051242:Caveolin 1 / Q000378:metabolism*; D002465:Cell Movement; D002478:Cells, Cultured; D004705:Endocytosis / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D044950:Flavanones / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D016166:Free Radical Scavengers / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D047468:Immunoprecipitation; D018613:Microscopy, Confocal; D010766:Phosphorylation; D057705:Transendothelial and Transepithelial Migration / Q000187:drug effects; D051176:beta Catenin / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D000199:Actins; D000418:Albumins; D051242:Caveolin 1; D044950:Flavanones; D016166:Free Radical Scavengers; D051176:beta Catenin; D006861:Hydrogen Peroxide; D048051:p38 Mitogen-Activated Protein Kinases; C085514:wogonin