title: [Simultaneous determination of four quinone compounds in carbonized Rubiae Radix et Rhizoma by UPLC].
abstract: OBJECTIVE
  To establish an UPLC method for simultaneous determination of purpuroxanthine, purpurin, 1,3,6-trihydroxy-2-methylanthraquinone, rubimaillin in carbonized Rubiae Radix et Rhizoma.
  
  
  METHODS
  The components were separated on acquity BEHC18 (2.1 mm x 50 mm, 1.7 microm) using methanol and 0.3% formic acid solution as the mobile phase; The flow rate was 0.2 mL x min(-1) and the volume of injection was 2 microL; the column temperature was maintained at 30 degrees C and the detective wavelength was set at 276 nm.
  
  
  RESULTS
  There were good liner relationships between the peak area and concentration at ranges of 0.68-34.44 mg x L(-1) (r = 0.9999), 0.66-33.2 mg x L(-1) (r = 0.9997), 0.68-34.08 mg x L(-1) (r = 0.9999), 1.07-53.52 mg x L(-1) (r = 0.9999) for purpuroxanthine, purpurin, 1,3,6-trihydroxy-2-methylanthraquinone, rubimaillin, respectively; the average recovery rates of purpuroxanthine, purpurin, 1,3,6-trihydroxy-2-methylanthraquinone, rubimaillin were 96.95%, 95.75%, 102.5%, 96.15%, respectively, with RSD less than 3%.
  
  
  CONCLUSIONS
  The established method was rapid and simple with good accuracy and reproducibility for the determination of carbonized Rubiae Radix et Rhizoma, the method was suitable for the quality control of carbonized Rubiae Radix et Rhizoma.
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China. nanjingchenxing@126.com
authors.forename: Xing
authors.initials: X
authors.lastname: Chen
authors.forename: Kan
authors.initials: K
authors.lastname: Wang
authors.forename: Ming-qiu
authors.initials: MQ
authors.lastname: Shan
authors.forename: An-wei
authors.initials: AW
authors.lastname: Ding
mesh_terms: D002851:Chromatography, High Pressure Liquid; D011809:Quinones / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry*; D045470:Rubia / Q000737:chemistry*
chemical_list: D011809:Quinones