title: [Effects of rosiglitazone on cholesterol contents and scavenger receptor class B type I expression in RAW264.7 foam cells].
abstract: OBJECTIVE
  To observe the effect of rosiglitazone on the content of cholesterol and expressions of Acy-coenzyme A: cholesterol acyltransferase 1 (ACAT-1) and scavenger receptor class B type I (SR-BI) in RAW264.7 macrophage-derived foam cells and explore the anti-atherosclerotic mechanism of rosiglitazone.
  
  
  METHODS
  RAW264.7 macrophages were incubated with oxidized low-density lipoproteins (ox-LDL) or with both ox-LDL and rosiglitazone (5, 10, or 20 µmol/L). Oil red O staining was used to observe the formation of foam cells, and cholesterol oxidase was used to determine the content of cellular cholesterol contents. Western blotting was used observe the expressions of ACAT-1 and SR-BI in RAW264.7 foam cells.
  
  
  RESULTS
  Compared with the control cells, RAW264.7 macrophage-derived foam cells showed significantly increased contents of total cholesterol and free cholesterol (P<0.01) and ACAT-1 expressions (P<0.05) with mildly increased SR-BI expression (P>0.05). Rosiglitazone treatments significantly lowered the contents of total cholesterol and free cholesterol (P<0.05), decreased the expression of ACAT-1 (P<0.05), and increased SR-BI expression (P<0.05) in the foam cells in a dose-dependent manner.
  
  
  CONCLUSIONS
  Rosiglitazone can decrease the contents of total and free cholesterol, down-regulate ACAT-1 expression and up-regulate SR-BI expression in the foam cells produce the anti-atherosclerotic effect.
authors.affiliation: First Clinical Medical College, Lanzhou University, Lanzhou 730000, China. xufang0625@163.com
authors.forename: Fang
authors.initials: F
authors.lastname: Xu
authors.forename: Ying
authors.initials: Y
authors.lastname: Meng
authors.forename: Zhilu
authors.initials: Z
authors.lastname: Wang
authors.forename: Wanling
authors.initials: W
authors.lastname: Li
authors.forename: Junzheng
authors.initials: J
authors.lastname: Jia
authors.forename: Wenfen
authors.initials: W
authors.lastname: Guo
authors.forename: Wanxia
authors.initials: W
authors.lastname: Xie
authors.forename: Haiying
authors.initials: H
authors.lastname: Hu
authors.forename: Xutang
authors.initials: X
authors.lastname: Hu
mesh_terms: D000101:Acetyl-CoA C-Acetyltransferase / Q000378:metabolism; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism*; D005487:Foam Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D000077154:Rosiglitazone; D051122:Scavenger Receptors, Class B / Q000378:metabolism*; D045162:Thiazolidinediones / Q000494:pharmacology*
chemical_list: D051122:Scavenger Receptors, Class B; D045162:Thiazolidinediones; D000077154:Rosiglitazone; D002784:Cholesterol; C506639:ACAT1 protein, human; D000101:Acetyl-CoA C-Acetyltransferase