title: In vitro antimicrobial activity of AH Plus, EndoREZ and Epiphany against microorganisms.
abstract: OBJECTIVE
  The aim of the present study was to evaluate the antimicrobial activity of endodontic sealers against microorganisms.
  
  
  METHODS
  The agar diffusion method was used. A double base layer of Mueller Hinton agar was done. The microorganisms used were: Candida albicans, Enterococcus faecalis, Escherichia coli and Staphylococcus aureus. The wells were obtained by removing a standardized portion of the agar. After the distribution of the sealers, Petri plates were incubated for 24 h. Inhibition halos formed around the wells were measured.
  
  
  RESULTS
  Epiphany did not show any antimicrobial activity on the tested microorganisms (without inhibition halo). The AH Plus showed the greatest inhibition halo on C. albicans followed by EndoREZ on S. aureus. EndoREZ also showed greater inhibition halo in comparison to AH Plus on E. faecalis and E. coli.
  
  
  CONCLUSIONS
  It could be concluded that AH Plus and EndoREZ showed antimicrobial activity against all the tested microorganisms. No antimicrobial activity was observed for Epiphany.
authors.affiliation: Department of Restorative Dentistry, School of Dentistry of São José dos Campos, São Paulo State University - UNESP, São José dos Campos, São Paulo, Brazil.
authors.forename: Lilian Eiko
authors.initials: LE
authors.lastname: Maekawa
authors.forename: Maria Renata Giazzi
authors.initials: MR
authors.lastname: Nassri
authors.forename: Camila Kaori
authors.initials: CK
authors.lastname: Ishikawa
authors.forename: Carolina
authors.initials: C
authors.lastname: Martins
authors.forename: Adriana
authors.initials: A
authors.lastname: Chung
authors.forename: Cristiane Yumi
authors.initials: CY
authors.lastname: Koga-Ito
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects; D003188:Composite Resins / Q000494:pharmacology*; D013293:Enterococcus faecalis / Q000187:drug effects; D004853:Epoxy Resins / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D008422:Materials Testing; D008826:Microbial Sensitivity Tests; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D003188:Composite Resins; C481259:EndoRez; D004853:Epoxy Resins; D012387:Root Canal Filling Materials; C486893:epiphany sealer; C534916:epoxy resin-based root canal sealer