title: Targeted adenovirus mediated inhibition of NF-κB-dependent inflammatory gene expression in endothelial cells in vitro and in vivo.
abstract: In chronic inflammatory diseases the endothelium expresses mediators responsible for harmful leukocyte infiltration. We investigated whether targeted delivery of a therapeutic transgene that inhibits nuclear factor κB signal transduction could silence the proinflammatory activation status of endothelial cells. For this, an adenovirus encoding dominant-negative IκB (dnIκB) as a therapeutic transgene was employed. Selectivity for the endothelial cells was achieved by introduction of antibodies specific for inflammatory endothelial adhesion molecules E-selectin or VCAM-1 chemically linked to the virus via polyethylene glycol. In vitro, the retargeted adenoviruses selectively infected cytokine-activated endothelial cells to express functional transgene. The comparison of transductional capacity of both retargeted viruses revealed that E-selectin based transgene delivery exerted superior pharmacological effects. Targeted delivery mediated dnIκB transgene expression in endothelial cells inhibited the induced expression of several inflammatory genes, including adhesion molecules, cytokines, and chemokines. In vivo, in mice suffering from glomerulonephritis, E-selectin-retargeted adenovirus selectively homed in the kidney to microvascular glomerular endothelium. Subsequent downregulation of endothelial adhesion molecule expression 2 days after induction of inflammation demonstrated the pharmacological potential of this gene therapy approach. The data justify further studies towards therapeutic virus design and optimization of treatment schedules to investigate their capacity to interfere with inflammatory disease progression.
authors.affiliation: Department of Pathology and Medical Biology, section Medical Biology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
authors.forename: J M
authors.initials: JM
authors.lastname: Kułdo
authors.forename: S A
authors.initials: SA
authors.lastname: Ásgeirsdóttir
authors.forename: P J
authors.initials: PJ
authors.lastname: Zwiers
authors.forename: A R
authors.initials: AR
authors.lastname: Bellu
authors.forename: M G
authors.initials: MG
authors.lastname: Rots
authors.forename: J A C
authors.initials: JA
authors.lastname: Schalk
authors.forename: K I
authors.initials: KI
authors.lastname: Ogawara
authors.forename: C
authors.initials: C
authors.lastname: Trautwein
authors.forename: B
authors.initials: B
authors.lastname: Banas
authors.forename: H J
authors.initials: HJ
authors.lastname: Haisma
authors.forename: G
authors.initials: G
authors.lastname: Molema
authors.forename: J A A M
authors.initials: JA
authors.lastname: Kamps
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D001667:Binding, Competitive; D018929:Cell Culture Techniques; D004195:Disease Models, Animal; D019040:E-Selectin / Q000235:genetics; D004730:Endothelium, Vascular / Q000187:drug effects / Q000276:immunology; D005260:Female; D015870:Gene Expression; D005921:Glomerulonephritis / Q000235:genetics / Q000628:therapy; D057809:HEK293 Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D020672:I-kappa B Proteins / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D011485:Protein Binding; D015398:Signal Transduction / Q000235:genetics; D019076:Transgenes; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics
chemical_list: D019040:E-Selectin; D020672:I-kappa B Proteins; D016328:NF-kappa B; D019010:Vascular Cell Adhesion Molecule-1