title: Imaging the cell surface and its organization down to the level of single molecules.
abstract: Determining the organization of key molecules on the surface of live cells in two dimensions and how this changes during biological processes, such as signalling, is a major challenge in cell biology and requires methods with nanoscale spatial resolution and high temporal resolution. Here, we review biophysical tools, based on scanning ion conductance microscopy and single-molecule fluorescence and the combination of both of these methods, which have recently been developed to address these issues. We then give examples of how these methods have been be applied to provide new insights into cell membrane organization and function, and discuss some of the issues that will need to be addressed to further exploit these methods in the future.
authors.affiliation: Department of Chemistry, Cambridge University, Cambridge CB2 1EW, UK. dk10012@cam.ac.uk
authors.forename: David
authors.initials: D
authors.lastname: Klenerman
authors.forename: Andrew
authors.initials: A
authors.lastname: Shevchuk
authors.forename: Pavel
authors.initials: P
authors.lastname: Novak
authors.forename: Yuri E
authors.initials: YE
authors.lastname: Korchev
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Davis
mesh_terms: D003585:Cell Biology; D002462:Cell Membrane / Q000737:chemistry*; D058948:Cell Tracking / Q000379:methods*; D002966:Clathrin / Q000737:chemistry; D051152:Clusterin / Q000737:chemistry; D004705:Endocytosis; D005453:Fluorescence; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry; D008856:Microscopy, Fluorescence / Q000379:methods; D019636:Neurodegenerative Diseases / Q000473:pathology; D025941:Protein Interaction Mapping; D021381:Protein Transport; D011948:Receptors, Antigen, T-Cell / Q000737:chemistry*
chemical_list: C495242:CLU protein, human; D002966:Clathrin; D051152:Clusterin; D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell