title: Model creation of moving redox reaction boundary in agarose gel electrophoresis by traditional potassium permanganate method.
abstract: A novel moving redox reaction boundary (MRRB) model was developed for studying electrophoretic behaviors of analytes involving redox reaction on the principle of moving reaction boundary (MRB). Traditional potassium permanganate method was used to create the boundary model in agarose gel electrophoresis because of the rapid reaction rate associated with MnO(4)(-) ions and Fe(2+) ions. MRB velocity equation was proposed to describe the general functional relationship between velocity of moving redox reaction boundary (V(MRRB)) and concentration of reactant, and can be extrapolated to similar MRB techniques. Parameters affecting the redox reaction boundary were investigated in detail. Under the selected conditions, good linear relationship between boundary movement distance and time were obtained. The potential application of MRRB in electromigration redox reaction titration was performed in two different concentration levels. The precision of the V(MRRB) was studied and the relative standard deviations were below 8.1%, illustrating the good repeatability achieved in this experiment. The proposed MRRB model enriches the MRB theory and also provides a feasible realization of manual control of redox reaction process in electrophoretic analysis.
authors.affiliation: Laboratory of Analytical Biochemistry and Bio-separation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
authors.forename: Hai-Yang
authors.initials: HY
authors.lastname: Xie
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Jia-Hao
authors.initials: JH
authors.lastname: Li
authors.forename: Liu-Yin
authors.initials: LY
authors.lastname: Fan
authors.forename: Cheng-Xi
authors.initials: CX
authors.lastname: Cao
mesh_terms: D004587:Electrophoresis, Agar Gel / Q000379:methods*; D008956:Models, Chemical; D010084:Oxidation-Reduction; D011196:Potassium Permanganate / Q000737:chemistry*
chemical_list: D011196:Potassium Permanganate