title: Hypotonic treatment prior to freezing improves cryoresistance of common carp (Cyprinus carpio L.) spermatozoa.
abstract: Post-thaw motility rate, curvilinear velocity (VCL), and fertilizing ability of carp spermatozoa can be improved by short-term treatment with moderately hypotonic media prior to freezing. Before cryopreservation, carp sperm samples were treated with NaCl solutions of differing osmolalities, ranging from 100 to 300mOsmkg(-1) for 10s, after which final osmolality was adjusted to 300mOsmkg(-1). The resulting sperm suspension was diluted 1:1 with cryoprotective medium and frozen using conventional techniques. Control samples were treated in the same way, without the pre-dilution step. Post-thaw motility rate in samples pretreated with 200mOsmkg(1) NaCl was significantly higher (44±10%) than in controls (21±15%) and samples pretreated with 100mOsmkg(-1) (25±15%) and 300mOsmkg(-1) (25±12%) NaCl. Significantly higher mean VCL were observed in samples pretreated with 100, 150, and 200mOsmkg(-1) (119±24, 118±22, and 115±32μms(-1), respectively) compared to controls (92±27μms(-1)). Fertilization rate of frozen-thawed sperm treated with 200mOsmkg(-1) solution of 2M NaCl was significantly higher (25±18%) than that of sperm treated with 300mOsmkg(-1) NaCl solutions (12±7%) and the control (9±6%).
authors.affiliation: University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic. bdzyuba@frov.jcu.cz
authors.forename: Borys
authors.initials: B
authors.lastname: Dzyuba
authors.forename: Jacky
authors.initials: J
authors.lastname: Cosson
authors.forename: Gunes
authors.initials: G
authors.lastname: Yamaner
authors.forename: Olga
authors.initials: O
authors.lastname: Bondarenko
authors.forename: Marek
authors.initials: M
authors.lastname: Rodina
authors.forename: David
authors.initials: D
authors.lastname: Gela
authors.forename: Volodymir
authors.initials: V
authors.lastname: Bondarenko
authors.forename: Anna
authors.initials: A
authors.lastname: Shaliutina
authors.forename: Otomar
authors.initials: O
authors.lastname: Linhart
mesh_terms: D000818:Animals; D002347:Carps / Q000502:physiology*; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000378:metabolism; D005260:Female; D005306:Fertilization; D007038:Hypotonic Solutions; D008297:Male; D009994:Osmolar Concentration; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D012965:Sodium Chloride / Q000378:metabolism*; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism
chemical_list: D003451:Cryoprotective Agents; D007038:Hypotonic Solutions; D012965:Sodium Chloride