title: Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples.
abstract: A novel clinical glucose biosensor fabricated using functionalized metalloid-polymer (silver-silica coated with polyethylene glycol) hybrid nanoparticles on the surface of a graphene oxide nanosheet is reported. The cyclic voltammetric response of glucose oxidase modification on the surface of a functionalized graphene oxide electrode showed a surface-confined reaction and an effective redox potential near zero volts, with a wide linearity of 0.1-20 mM and a sensitivity of 7.66 μA mM(-1) cm(-2). The functionalized graphene oxide electrode showed a better electrocatalytic response toward oxidation of H(2)O(2) and reduction of oxygen. The practical applicability of the functionalized graphene oxide electrode was demonstrated by measuring the peak current against multiple urine and serum samples from diabetic patients. This new hybrid nanoarchitecture combining a three-dimensional metalloid-polymer hybrid and two-dimensional graphene oxide provided a thin solid laminate on the electrode surface. The easy fabrication process and retention of bioactive immobilized enzymes on the functionalized graphene oxide electrode could potentially be extended to detection of other biomolecules, and have broad applications in electrochemical biosensing.
authors.affiliation: Department of Bionanotechnology, Gachon University, Gyeonggi-Do.
authors.forename: Murugan
authors.initials: M
authors.lastname: Veerapandian
authors.forename: Yeong-Tai
authors.initials: YT
authors.lastname: Seo
authors.forename: Hyunkyung
authors.initials: H
authors.lastname: Shin
authors.forename: Kyusik
authors.initials: K
authors.lastname: Yun
authors.forename: Min-Ho
authors.initials: MH
authors.lastname: Lee
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D001786:Blood Glucose / Q000032:analysis*; D003217:Conductometry / Q000295:instrumentation*; D003920:Diabetes Mellitus / Q000097:blood / Q000175:diagnosis* / Q000652:urine; D005947:Glucose; D006029:Glycosuria / Q000175:diagnosis*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D001786:Blood Glucose; D006108:Graphite; D005947:Glucose