title: Fibronectin binding is required for acquisition of mesenchymal/endothelial differentiation potential in human circulating monocytes.
abstract: We previously reported monocyte-derived multipotential cells (MOMCs), which include progenitors capable of differentiating into a variety of mesenchymal cells and endothelial cells. In vitro generation of MOMCs from circulating CD14(+) monocytes requires their binding to extracellular matrix (ECM) protein and exposure to soluble factor(s) derived from circulating CD14(-) cells. Here, we investigated the molecular factors involved in MOMC generation by examining the binding of monocytes to ECM proteins. We found that MOMCs were obtained on the fibronectin, but not on type I collagen, laminin, or poly-L-lysine. MOMC generation was followed by changes in the expression profiles of transcription factors and was completely inhibited by either anti-α(5) integrin antibody or a synthetic peptide that competed with the RGD domain for the β(1)-integrin binding site. These results indicate that acquisition of the multidifferentiation potential by circulating monocytes depends on their binding to the RGD domain of fibronectin via cell-surface α(5)β(1) integrin.
authors.affiliation: Division of Rheumatology, Department of Internal Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Seta
authors.forename: Yuka
authors.initials: Y
authors.lastname: Okazaki
authors.forename: Keisuke
authors.initials: K
authors.lastname: Izumi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyazaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Kato
authors.forename: Masataka
authors.initials: M
authors.lastname: Kuwana
mesh_terms: D000328:Adult; D001665:Binding Sites / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005353:Fibronectins / Q000276:immunology*; D006801:Humans; D039081:Integrin alpha5beta1 / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D009000:Monocytes / Q000166:cytology* / Q000378:metabolism; D039902:Multipotent Stem Cells / Q000166:cytology / Q000276:immunology; D009842:Oligopeptides / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D055815:Young Adult
chemical_list: D024042:Collagen Type I; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D039081:Integrin alpha5beta1; D007797:Laminin; D018950:Lipopolysaccharide Receptors; D009842:Oligopeptides; D014157:Transcription Factors; C047981:arginyl-glycyl-aspartic acid; D008239:Lysine