title: Analysis by RP-HPLC of mangiferin component correlation between medicinal loranthus and their mango host trees.
abstract: This study proposes a reversed-phase high-performance liquid chromatography (RP-HPLC) method for the determination of mangiferin in medicinal loranthus shrubs and their mango or non-mango host trees. Mangiferin in samples was extracted with a solution of 40% methanol. Analytical determination was conducted by RP-HPLC with ultraviolet detection at 258 nm. Chromatographic separation was achieved on an Inertsil ODS-SP column (250 × 4.6 mm, 5 µm) by isocratic elution with methanol-0.1% aqueous phosphoric acid (31:69, v/v). Mangiferin contents were 5.04 to 18.95 mg/g in mango trees and 0.44 to 3.72 mg/g in medicinal loranthus parasitized on mango host trees. Mangiferin could not be detected in non-mango trees and their loranthus shrubs. This study indicated that host trees could affect the quality of medicinal loranthus by transporting host-inherent components into loranthus.
authors.affiliation: 1Guangxi Traditional Chinese Medicine University, Nanning, 530001, PR China.
authors.forename: Xiejun
authors.initials: X
authors.lastname: Zhang
authors.forename: Benwei
authors.initials: B
authors.lastname: Su
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Li
authors.forename: Dong
authors.initials: D
authors.lastname: Lu
authors.forename: Kaixin
authors.initials: K
authors.lastname: Zhu
authors.forename: Hehuan
authors.initials: H
authors.lastname: Pei
authors.forename: Minghui
authors.initials: M
authors.lastname: Zhao
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D006790:Host-Parasite Interactions; D057230:Limit of Detection; D016014:Linear Models; D028183:Loranthaceae / Q000737:chemistry*; D031022:Mangifera / Q000469:parasitology*; D010936:Plant Extracts / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D044004:Xanthones / Q000032:analysis* / Q000737:chemistry
chemical_list: D010936:Plant Extracts; D044004:Xanthones; C013592:mangiferin