title: Effects of sulfated polysaccharide from Masson pine (Pinus massoniana) pollen on the proliferation and cell cycle of HepG2 cells.
abstract: OBJECTIVE
  To explore the inhibitory effect of sulfated polysaccharide from Masson pine (Pinus massoniana) pollen (SPPM60) on G2/M phase of human liver cancer HepG2 cells and its mechanism.
  
  
  METHODS
  The proliferation rate of HepG2 cells was evaluated by methyl thiazolyl tetrazolium (MTT) colorimetric assay. The cycles of HepG2 cells were measured by flow cytometry when 200μg/ml concentration of SPPM60 was adopted, the expression of the genes related to cell cycle was detected by real-time PCR.
  
  
  RESULTS
  SPPM60 inhibited the proliferation of HepG2 cells and the inhibition rate was elevated with increase of SPPM60 concentration. After treatment with 200μg/ml of SPPM60, the percentage of S phase cells was decreased, but that of G2/M phase was significantly increased (72h vs control: 32.96±0.33% vs 18.59±0.04%, 3.44±0.05% vs 18.30±0.08%, P<0.01). The results of real-time PCR showed that SPPM60 could down-regulate the mRNA levels of CDK1 and CyclinB (P<0.01), and up-regulate the expression of p53 and p21 (P<0.05).
  
  
  CONCLUSIONS
  SPPM60 causes arrest of HepG2 cells at G2/M phase, and the mechanism is related to the down-regulation of CDK1 and CyclinB and up-regulation of p53 and p21 expression.
authors.affiliation: Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life Science, Shandong Normal University, Jinan 250014, China.
authors.forename: Hui-Li
authors.initials: HL
authors.lastname: Chu
authors.forename: Hua
authors.initials: H
authors.lastname: Mao
authors.forename: Wei
authors.initials: W
authors.lastname: Feng
authors.forename: Jing-wen
authors.initials: JW
authors.lastname: Liu
authors.forename: Yue
authors.initials: Y
authors.lastname: Geng
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects* / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D028223:Pinus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D011058:Pollen / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D013463:Sulfuric Acid Esters
chemical_list: D018797:Cell Cycle Proteins; D010936:Plant Extracts; D011134:Polysaccharides; D013463:Sulfuric Acid Esters