title: Synthesis of stable carboxy-terminated NaYF4: Yb3+, Er3+@SiO2 nanoparticles with ultrathin shell for biolabeling applications.
abstract: Here, a two-step method has been developed for synthesizing carboxy-terminated NaYF(4): Yb(3+), Er(3+)@SiO(2) core@shell nanoparticles (UCNP@SiO(2)) with ultrathin shell (1.5 nm). First, the NaYF(4): Yb(3+), Er(3+) upconverting nanoparticles (UCNPs) were prepared using solvothermal technology; then, silica shells (SiO(2)) were deposited on the nanocrystals to form core-shell structures by the hydrolysis of tetraethylorthosilicate (TEOS). The ultrathin SiO(2) shell was obtained by increasing surfactant amount and decreasing TEOS amount in the reaction mixture. Carboxyethylsilanetriol (CTES) was used to generate the carboxy group on the particle surface. The carboxy-terminated UCNP@SiO(2) are ideally suited for biolabeling and bioimaging applications because the as-prepared nanoparticles have extreme colloidal and optical stabilities, and the carboxy groups on the particle surface easily react with amino residues of biomolecules. As an example, we reported on the interactions of Ricinus Communis Agglutinin (RCA 120) conjugated UCNP@SiO(2) with HeLa cells. The excellent performance of the RCA 120 conjugated UCNP@SiO(2) in cellular fluorescence imaging was demonstrated.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China.
authors.forename: Fuyao
authors.initials: F
authors.lastname: Liu
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhao
authors.forename: Hongpeng
authors.initials: H
authors.lastname: You
authors.forename: Zhenxin
authors.initials: Z
authors.lastname: Wang
mesh_terms: D005459:Fluorides / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D037121:Plant Lectins / Q000493:pharmacokinetics*; D012822:Silicon Dioxide / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods*; D015019:Yttrium / Q000737:chemistry*
chemical_list: D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; C525362:sodium yttriumtetrafluoride; D015019:Yttrium; D012822:Silicon Dioxide; D005459:Fluorides