title: Functional human Th17 clones with WT1-specific helper activity.
abstract: Th17 plays important roles in the pathogenesis of various inflammatory and autoimmune diseases. Although the importance of Th17 in tumor immunity has also been suggested, precise roles of tumor-associated antigen-specific Th17 still remain poorly understood, especially in humans. We previously identified WT1332, a 16-mer helper epitope derived from tumor-associated antigen Wilms' tumor gene 1 (WT1) product, and WT1332-specific Th1 clones were established. In the present study, WT1-specific Th17 clones were established by the stimulation of peripheral blood mononuclear cells with the WT1332 helper peptide under human Th17-polarizing conditions. The WT1-specific Th17 clone exhibited the helper function for proliferation of conventional CD4(+) T cells in the antigenic stimulation-specific manner. This is the first report of establishment of functional Th17 clones with both antigen (WT1332) specificity and antigen-specific helper activity. Th17 clones established here and the method to establish antigen-specific Th17 clones should be a useful tool to further analyze the roles of human Th17 in tumor immunity.
authors.affiliation: Department of Respiratory Medicine, Allergy and Rheumatic Diseases, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka, Japan. stachino@imed3.med.osaka-u.ac.jp
authors.forename: Sho
authors.initials: S
authors.lastname: Tachino
authors.forename: Fumihiro
authors.initials: F
authors.lastname: Fujiki
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Oka
authors.forename: Akihiro
authors.initials: A
authors.lastname: Tsuboi
authors.forename: Soyoko
authors.initials: S
authors.lastname: Morimoto
authors.forename: Yu-Hung
authors.initials: YH
authors.lastname: Lin
authors.forename: Taichi
authors.initials: T
authors.lastname: Tamanaka
authors.forename: Kenta
authors.initials: K
authors.lastname: Kondo
authors.forename: Hiroko
authors.initials: H
authors.lastname: Nakajima
authors.forename: Sumiyuki
authors.initials: S
authors.lastname: Nishida
authors.forename: Naoki
authors.initials: N
authors.lastname: Hosen
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Oji
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kumanogoh
authors.forename: Haruo
authors.initials: H
authors.lastname: Sugiyama
mesh_terms: D002999:Clone Cells; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D006682:HLA-DP Antigens / Q000276:immunology; D059866:HLA-DQ beta-Chains / Q000276:immunology; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D020381:Interleukin-17 / Q000096:biosynthesis / Q000276:immunology; D058504:Th17 Cells / Q000166:cytology / Q000276:immunology*; D025721:WT1 Proteins / Q000276:immunology*
chemical_list: D018984:Epitopes, T-Lymphocyte; D006682:HLA-DP Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D020381:Interleukin-17; D025721:WT1 Proteins