title: Microfluidic free-flow electrophoresis chips with an integrated fluorescent sensor layer for real time pH imaging in isoelectric focusing.
abstract: Functional microfluidic free-flow electrophoresis chips with integrated fluorescent pH sensors are presented. Polyethylene glycol based structures were fabricated that allowed for integration of both functions on a single microchip. Microchips were applied in free-flow isoelectric focusing of model compounds and proteins with on-line monitoring of pH during microscale electrophoretic separation.
authors.affiliation: Institut für Analytische Chemie, Universität Leipzig, Linnéstr. 3, D-04103 Leipzig, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Jezierski
authors.forename: Detlev
authors.initials: D
authors.lastname: Belder
authors.forename: Stefan
authors.initials: S
authors.lastname: Nagl
mesh_terms: D000818:Animals; D002417:Cattle; D003207:Conalbumin / Q000302:isolation & purification; D046209:Electrophoresis, Microchip / Q000295:instrumentation*; D004867:Equipment Design; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000295:instrumentation*; D011092:Polyethylene Glycols / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000302:isolation & purification
chemical_list: D005456:Fluorescent Dyes; D003207:Conalbumin; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols