title: Enhanced ethanol production from wheat straw by integrated storage and pre-treatment (ISP).
abstract: Integrated storage and pre-treatment (ISP) combines biopreservation of moist material under airtight conditions and pre-treatment. Moist wheat straw was inoculated with the biocontrol yeast Wickerhamomyces anomalus, the xylan degrading yeast Scheffersomyces stipitis or a co-culture of both. The samples and non-inoculated controls were stored at 4 or 15 °C. The non-inoculated controls were heavily contaminated with moulds, in contrast to the samples inoculated with W. anomalus or S. stipitis. These two yeasts were able to grow on wheat straw as sole source of nutrients. When ethanol was produced from moist wheat straw stored for four weeks at 4 °C with S. stipitis, an up to 40% enhanced yield (final yield 0.15 g ethanol per g straw dry weight) was obtained compared to a dry sample (0.107 g/g). In all other moist samples, stored for four weeks at 4 °C or 15 °C, 6-35% higher yields were obtained. Thus, energy efficient bio-preservation can improve the pre-treatment efficiency for lignocellulose biomass, which is a critical bottleneck in its conversion to biofuels.
authors.affiliation: Swedish University of Agricultural Sciences, Department of Microbiology, Box 7025, SE-75007 Uppsala, Sweden. Volkmar.Passoth@slu.se
authors.forename: Volkmar
authors.initials: V
authors.lastname: Passoth
authors.forename: Muhammad Rizwan
authors.initials: MR
authors.lastname: Tabassum
authors.forename: Harikrishnan A S
authors.initials: HA
authors.lastname: Nair
authors.forename: Matilda
authors.initials: M
authors.lastname: Olstorpe
authors.forename: Ievgeniia
authors.initials: I
authors.lastname: Tiukova
authors.forename: Jerry
authors.initials: J
authors.lastname: Ståhlberg
mesh_terms: D056804:Biofuels; D018533:Biomass; D018920:Coculture Techniques; D055165:Debaryomyces / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005656:Fungal Proteins / Q000378:metabolism; D006358:Hot Temperature; D006813:Humidity; D008031:Lignin / Q000378:metabolism; D010843:Pichia / Q000378:metabolism*; D018547:Plant Stems / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D011309:Preservation, Biological / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013464:Sulfuric Acids / Q000494:pharmacology; D013696:Temperature; D014908:Triticum / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology
chemical_list: D056804:Biofuels; D005656:Fungal Proteins; D013464:Sulfuric Acids; C036909:lignocellulose; D000431:Ethanol; D008031:Lignin; C033158:sulfuric acid