title: MicroRNAome and expression profile of developing tooth germ in miniature pigs.
abstract: MicroRNAs (miRNAs) play important roles in the regulation of rodent tooth development, but little is known about their role in tooth development in large mammals. We identified 637 unique miRNA sequences in a large-scale screen for miRNA expression profiles in the developing lower deciduous molars of miniature pigs (Sus scrofa) using Illumina Solexa deep sequencing. These candidate miRNAs and another 105 known Sus scrofa miRNAs were included in the custom-designed microarray and used to analyze the miRNA expression profile in the bud, cap, early bell, and late bell stages of tooth development. Microarray analysis revealed 166 transcripts that were differentially expressed in the four stages. Bioinformatic analysis identified 18 key miRNAs, including let-7f, miR-128, miR-200b, and miR-200c, that might play key roles in tooth development. Taken together, our results not only identified the specific microRNAome and expression profile in developing lower deciduous molars of the miniature pig, but they also provided useful information for investigating the molecular mechanism of tooth development in the miniature pig.
authors.affiliation: Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China.
authors.forename: Ang
authors.initials: A
authors.lastname: Li
authors.forename: Tieli
authors.initials: T
authors.lastname: Song
authors.forename: Fu
authors.initials: F
authors.lastname: Wang
authors.forename: Dayong
authors.initials: D
authors.lastname: Liu
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Fan
authors.forename: Chunmei
authors.initials: C
authors.lastname: Zhang
authors.forename: Junqi
authors.initials: J
authors.lastname: He
authors.forename: Songlin
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D019295:Computational Biology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D015203:Reproducibility of Results; D034421:Sus scrofa; D014083:Tooth Germ / Q000378:metabolism*
chemical_list: D035683:MicroRNAs