title: Diagnosis of meningococcal infection by QPCR: detection and quantification of DNA / Neisseria meningitidis.
abstract: We present the case of a patient with clinical signs of meningococcemia and negative bacterial culture. Microbiological diagnosis was possible only by testing with real time PCR technique the cerebrospinal fluid and blood samples. Both pathologic samples were positive for Neisseria meningitidis by this rapid and sensitive diagnostic method.
authors.affiliation: Discipline of Infectious Diseases, University of Medicine and Pharmacy, Grigore T. Popa-Iaşi School of Medicine.
authors.forename: Roxana Elena
authors.initials: RE
authors.lastname: Nemescu
authors.forename: Ramona Gabriela
authors.initials: RG
authors.lastname: Ursu
authors.forename: Carmen Mihaela
authors.initials: CM
authors.lastname: Dorobăţ
authors.forename: Luminiţa Smaranda
authors.initials: LS
authors.lastname: Iancu
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D002443:Ceftriaxone / Q000627:therapeutic use; D004269:DNA, Bacterial / Q000302:isolation & purification; D003937:Diagnosis, Differential; D005076:Exanthema / Q000382:microbiology; D006801:Humans; D007223:Infant; D008297:Male; D008580:Meningism / Q000382:microbiology; D008589:Meningococcal Infections / Q000097:blood / Q000134:cerebrospinal fluid / Q000175:diagnosis* / Q000188:drug therapy; D009345:Neisseria meningitidis / Q000235:genetics / Q000302:isolation & purification*; D011237:Predictive Value of Tests; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D002443:Ceftriaxone