title: The role of pectic composition of cell walls in the determination of the new shape-functional design in galls of Baccharis reticularia (Asteraceae).
abstract: The pectic composition of cell wall is altered during the processes of cell differentiation, plant growth, and development. These alterations may be time-dependent, and fluctuate in distinct regions of the same cell or tissue layer, due to the biotic stress caused by the activity of the gall inducer. Among the roles of the pectins in cell wall, elasticity, rigidity, porosity, and control of cell death may be crucial during gall development. Galls on Baccharis reticularia present species-specific patterns of development leading to related morphotypes where pectins were widely detected by Ruthenium red, and the pectic epitopes were labeled with specific monoclonal antibodies (LM1, LM2, LM5, LM6, JIM5, and JIM7) in distinct sites of the non-galled and the galled tissues. In the studied system B. reticularia, the epitopes for extensins were not labeled in the non-galled tissues, as well as in those of the rolling and kidney-shaped galls. The high methyl-esterified homogalacturonans (HGA) were labeled all over the tissues either of non-galled leaves or of the three gall morphotypes, while the intense labeling for arabinogalactans was obtained just in the rolling galls. The pectic composition of non-galled leaves denotes their maturity. The kidney-shaped gall was the most similar to the non-galled leaves. The pectic dynamics in the gall tissues was particularly altered in relation to low methyl-esterified HGA, which confers elasticity and expansion, as well as porosity and adhesion to cell walls, and are related to the homogenization and hypertrophy of gall cortex, and to translocation of solutes to the larval chamber. Herein, the importance of the pectic dynamics of cell walls to the new functional design established during gall development is discussed for the first time. The repetitive developmental patterns in galls are elegant models for studies on cell differentiation.
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais - UFMG-ICB, Belo Horizonte, MG, CEP: 31270-901, Brasil.
authors.forename: Anete Teixeira
authors.initials: AT
authors.lastname: Formiga
authors.forename: Denis Coelho
authors.initials: DC
authors.lastname: de Oliveira
authors.forename: Bruno Garcia
authors.initials: BG
authors.lastname: Ferreira
authors.forename: Thiago Alves
authors.initials: TA
authors.lastname: Magalhães
authors.forename: Ariane Chagas
authors.initials: AC
authors.lastname: de Castro
authors.forename: G Wilson
authors.initials: GW
authors.lastname: Fernandes
authors.forename: Rosy Mary Dos Santos
authors.initials: RM
authors.lastname: Isaias
mesh_terms: D019659:Asteraceae / Q000378:metabolism*; D031175:Baccharis / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism; D007150:Immunohistochemistry; D010368:Pectins / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism
chemical_list: D010368:Pectins