title: The average IFN-γ secreting capacity of specific CD8(+) T cells is compromised while increasing copies of a single T cell epitope encoded by DNA vaccine.
abstract: Previous studies suggested that both the frequency and the mean fluorescence intensity (MFI) of cytokine secreting T cells could be of great value for immunogenicity evaluation of a vaccine. In this study, by constructing epitope-based DNA vaccines encoding a previously identified CD8(+) T cell epitope, we investigated the influence of multiplying epitope copies on both the frequency and the MFI of specific IFN-γ secreting CD8(+) T cells. We found that frequencies of specific CD8(+) T cell could be improved by multiplying epitope copies, while the MFI of IFN-γ secreted by epitope-specific CD8(+) T cells decreased synchronously. And further analysis showed that the decrease of MFI was not caused by the functional avidity variation of CD8(+) T cell receptor.
authors.affiliation: Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Wan
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Haizhu
authors.initials: H
authors.lastname: Zhou
authors.forename: Zhidong
authors.initials: Z
authors.lastname: Hu
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Ren
authors.forename: Jianqing
authors.initials: J
authors.lastname: Xu
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011897:Random Allocation; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D018984:Epitopes, T-Lymphocyte; D011948:Receptors, Antigen, T-Cell; D019444:Vaccines, DNA; D007371:Interferon-gamma