title: Extracellular enzyme production and phylogenetic distribution of yeasts in wastewater treatment systems.
abstract: The abilities of yeasts to produce different extracellular enzymes and their distribution characteristics were studied in municipal, inosine fermentation, papermaking, antibiotic fermentation, and printing and dyeing wastewater treatment systems. The results indicated that of the 257 yeasts, 16, 14, 55, and 11 produced lipase, protease, manganese dependant peroxidase (MnP), and lignin peroxidase (LiP), respectively. They were distributed in 12 identified and four unidentified genera, in which Candida rugosa (AA-M17) and an unidentified Saccharomycetales (AA-Y5), Pseudozyma sp. (PH-M15), Candida sp. (MO-Y11), and Trichosporon montevideense (MO-M16) were shown to have the highest activity of lipase, protease, Mnp, and LiP, respectively. No yeast had amylase, cellulose, phytase, or laccase activity. Although only 60 isolates produced ligninolytic enzymes, 249 of the 257 yeasts could decolorize different dyes through the mechanism of biodegradation (222 isolates) or bio-sorption. The types of extracellular enzymes that the yeasts produced were significantly shaped by the types of wastewater treated.
authors.affiliation: College of Life Sciences, Henan Normal University, Xinxiang 453007, China. yangqx66@163.com
authors.forename: Qingxiang
authors.initials: Q
authors.lastname: Yang
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Xueling
authors.initials: X
authors.lastname: Li
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.forename: Siwei
authors.initials: S
authors.lastname: Ren
authors.forename: Xuanyu
authors.initials: X
authors.lastname: Chen
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Xu
authors.forename: Hongxin
authors.initials: H
authors.lastname: Hao
authors.forename: Hailei
authors.initials: H
authors.lastname: Wang
mesh_terms: D004798:Enzymes / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D059013:Microbial Consortia / Q000502:physiology*; D010802:Phylogeny; D062065:Wastewater / Q000382:microbiology*; D018508:Water Purification / Q000379:methods*; D015003:Yeasts / Q000302:isolation & purification* / Q000502:physiology*
chemical_list: D004798:Enzymes; D005656:Fungal Proteins; D062065:Wastewater