title: Alpha-tocopherol succinate-mobilized progenitors improve intestinal integrity after whole body irradiation.
abstract: OBJECTIVE
  The objective of this study was to elucidate the action of α-tocopherol succinate (TS)- and AMD3100-mobilized progenitors in mitigating radiation-induced injuries.
  
  
  METHODS
  CD2F1 mice were exposed to a high dose of radiation and then transfused intravenously with 5 million peripheral blood mononuclear cells (PBMC) from TS- and AMD3100-injected mice after irradiation. Intestinal and splenic tissues were harvested after irradiation and cells of those tissues were analyzed for markers of apoptosis and mitosis. Bacterial translocation from gut to heart, spleen, and liver in TS-treated and irradiated mice was evaluated by bacterial culture.
  
  
  RESULTS
  We observed that the infusion of PBMC from TS- and AMD3100-injected mice significantly inhibited apoptosis, increased cell proliferation in the analyzed tissues of recipient mice, and inhibited bacterial translocation to various organs compared to mice receiving cells from vehicle-mobilized cells. This study further supports our contention that the infusion of TS-mobilized progenitor-containing PBMC acts as a bridging therapy by inhibiting radiation-induced apoptosis, enhancing cell proliferation, and inhibiting bacterial translocation in irradiated mice.
  
  
  CONCLUSIONS
  We suggest that this novel bridging therapeutic approach that involves the infusion of TS-mobilized hematopoietic progenitors following acute radiation injury might be applicable to humans as well.
authors.affiliation: Armed Forces Radiobiology Research Institute, Bethesda, MD 20889-5603, USA. vijay.singh@usuhs.edu
authors.forename: Vijay K
authors.initials: VK
authors.lastname: Singh
authors.forename: Stephen Y
authors.initials: SY
authors.lastname: Wise
authors.forename: Pankaj K
authors.initials: PK
authors.lastname: Singh
authors.forename: Ana
authors.initials: A
authors.lastname: Posarac
authors.forename: Oluseyi O
authors.initials: OO
authors.lastname: Fatanmi
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Ducey
authors.forename: David L
authors.initials: DL
authors.lastname: Bolduc
authors.forename: Thomas B
authors.initials: TB
authors.lastname: Elliott
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Seed
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D001419:Bacteria / Q000187:drug effects / Q000528:radiation effects; D002465:Cell Movement / Q000187:drug effects* / Q000528:radiation effects; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D053960:DNA Breaks / Q000187:drug effects / Q000528:radiation effects; D004731:Endotoxins / Q000097:blood; D005720:Gamma Rays / Q000009:adverse effects; D007422:Intestines / Q000166:cytology / Q000187:drug effects* / Q000382:microbiology / Q000528:radiation effects*; D007583:Jejunum / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D051379:Mice; D011832:Radiation Injuries / Q000517:prevention & control; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D014916:Whole-Body Irradiation / Q000009:adverse effects*; D024502:alpha-Tocopherol / Q000494:pharmacology*
chemical_list: D004731:Endotoxins; D011837:Radiation-Protective Agents; D024502:alpha-Tocopherol