title: Antibodies recognizing specific Mycobacterium avium subsp. paratuberculosis's MAP3738c protein in type 1 diabetes mellitus children are associated with serum Th1 (CXCL10) chemokine.
abstract: Recently Mycobacterium avium subsp. paratuberculosis (Map) was associated to type 1 diabetes mellitus (T1DM). In this study we investigated for Map presence in children affected by T1DM compared to healthy children. A pool of 212 sera from T1DM children at onset was compared to sera from 57 healthy children for humoral immune response towards the Map specific protein MAP3738c by ELISA. Serum concentrations of CXCL10 (pro-Th1) and CCL2 (pro-Th2) chemokines were also measured in both sera pool. Results showed that T1DM children had a stronger seropositivity towards MAP3738c protein compared to healthy children. Data highlighted also the correlation between serum activity of T1DM patients towards the specific protein of Map and the increase of CXCL10 concentration if compared to non-diabetic subjects. In conclusion, an immune response to Map in T1DM patients at onset was observed and this may indicate a role of the bacterium in triggering or precipitating the disease.
authors.affiliation: Department of Biomedical Sciences, Division of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Cossu
authors.forename: Ele
authors.initials: E
authors.lastname: Ferrannini
authors.forename: Poupak
authors.initials: P
authors.lastname: Fallahi
authors.forename: Alessandro
authors.initials: A
authors.lastname: Antonelli
authors.forename: Leonardo A
authors.initials: LA
authors.lastname: Sechi
mesh_terms: D000293:Adolescent; D000907:Antibodies, Bacterial / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D001426:Bacterial Proteins / Q000097:blood / Q000276:immunology*; D016022:Case-Control Studies; D054357:Chemokine CXCL10 / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000276:immunology* / Q000382:microbiology; D005260:Female; D006801:Humans; D008297:Male; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology*; D011994:Recombinant Proteins / Q000097:blood / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; D011994:Recombinant Proteins