title: Antifungal properties of durancins isolated from Enterococcus durans A5-11 and of its synthetic fragments.
abstract: The aim of this work was to study the antifungal properties of durancins isolated from Enterococcus durans A5-11 and of their chemically synthesized fragments. Enterococcus durans A5-11 is a lactic acid bacteria strain isolated from traditional Mongolian airag cheese. This strain inhibits the growth of several fungi including Fusarium culmorum, Penicillium roqueforti and Debaryomyces hansenii. It produces two bacteriocins: durancin A5-11a and durancin A5-11b, which have similar antimicrobial properties. The whole durancins A5-11a and A5-11b, as well as their N- and C-terminal fragments were synthesized, and their antifungal properties were studied. C-terminal fragments of both durancins showed stronger antifungal activities than other tested peptides. Treatment of D. hansenii LMSA2.11.003 strain with 2 mmol l(-1) of the synthetic peptides led to the loss of the membrane integrity and to several changes in the ultra-structure of the yeast cells. Chemically synthesized durancins and their synthetic fragments showed different antimicrobial properties from each other. N-terminal peptides show activities against both bacterial and fungal strains tested. C-terminal peptides have specific activities against tested fungal strain and do not show antibacterial activity. However, the C-terminal fragment enhances the activity of the N-terminal fragment in the whole bacteriocins against bacteria.
authors.affiliation: UR 1268 Biopolymères Interactions Assemblages, Institut National de Recherche Agronomique, équipe Fonctions et Interactions des Protéines, Nantes Cedex 3, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Belguesmia
authors.forename: Y
authors.initials: Y
authors.lastname: Choiset
authors.forename: H
authors.initials: H
authors.lastname: Rabesona
authors.forename: M
authors.initials: M
authors.lastname: Baudy-Floc'h
authors.forename: G
authors.initials: G
authors.lastname: Le Blay
authors.forename: T
authors.initials: T
authors.lastname: Haertlé
authors.forename: J-M
authors.initials: JM
authors.lastname: Chobert
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000935:Antifungal Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001430:Bacteriocins / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002611:Cheese / Q000382:microbiology; D055165:Debaryomyces / Q000187:drug effects* / Q000648:ultrastructure; D016983:Enterococcus / Q000302:isolation & purification / Q000378:metabolism; D005658:Fungi / Q000187:drug effects*; D008087:Listeria / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D001430:Bacteriocins; D010446:Peptide Fragments