title: Studies on α-glucosidase inhibition and anti-glycation potential of Iris loczyi and Iris unguicularis.
abstract: OBJECTIVE
  One of the aims of this research work is the isolation and identification of various constituents of two medicinally important plants (Iris loczyi and Iris unguicularis). Secondly, the prime aim is the biological evaluation of these natural products to discover new potential inhibitors of α-glucosidase enzyme and protein glycation.
  
  
  METHODS
  Plants of the genus Iris contain a variety of secondary metabolites. Chromatographic techniques were applied for the isolation of different compounds from Iris loczyi and Iris unguicularis. All the isolated compounds were screened for their α-glucosidase enzyme inhibition and antiglycation potential.
  
  
  RESULTS
  It is shown in the results that two compounds (Kaempferol and 8-Methoxyeriodictyol) isolated from plant Iris unguicularis and compounds (Arborinone and 5,7-dihydroxy-2',6-dimethoxyisoflavone) isolated from plant Iris loczyi possess promising activity against α-glucosidase enzyme as compare to acarbose which is used as a standard α-glucosidase inhibitor in this study. A flavanone (2',5-dihydroxy-6,7-methylenedioxy) isolated from Iris loczyi was explored as most active anti-glycating agent.
  
  
  CONCLUSIONS
  α-Glucosidase enzyme is a therapeutic target to treat carbohydrate mediated diseases. In this study various inhibitors of α-glucosidase are identified which might be important for the management of diabetes. Similarly, antiglycation agents may have application for the management of late diabetic complications.
authors.affiliation: H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan.
authors.forename: Mohmmed
authors.initials: M
authors.lastname: Mosihuzzman
authors.forename: Suad
authors.initials: S
authors.lastname: Naheed
authors.forename: Sumaira
authors.initials: S
authors.lastname: Hareem
authors.forename: Sumaira
authors.initials: S
authors.lastname: Talib
authors.forename: Ghulam
authors.initials: G
authors.lastname: Abbas
authors.forename: Shamsun Nahar
authors.initials: SN
authors.lastname: Khan
authors.forename: Muhammad Iqbal
authors.initials: MI
authors.lastname: Choudhary
authors.forename: Bilge
authors.initials: B
authors.lastname: Sener
authors.forename: Rasool Baksh
authors.initials: RB
authors.lastname: Tareen
authors.forename: Mudassir
authors.initials: M
authors.lastname: Israr
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002417:Cattle; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification* / Q000627:therapeutic use; D065089:Glycoside Hydrolase Inhibitors; D006031:Glycosylation / Q000187:drug effects; D031323:Iris Plant / Q000737:chemistry*; D008659:Metabolic Diseases / Q000188:drug therapy / Q000201:enzymology; D000520:alpha-Glucosidases / Q000737:chemistry / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D000520:alpha-Glucosidases