title: Association of HvLDI with limit dextrinase activity and malt quality in barley.
abstract: Limit dextrinase (LD) is a unique de-branching enzyme involved in starch mobilization of barley grains during malting, and closely related to malt quality. Genotypic variation of LD activity is controlled by genetic factors and also affected by environmental conditions. Correlation analysis between LD activity and four malt quality parameters showed that LD activity was positively correlated with diastatic power, Kolbach index and the quality of malt extract, while negatively correlated with viscosity. The structure-based association analysis demonstrated that HvLDI, a gene encoding limit dextrinase inhibitor, was a major determinant of LD activity and malt quality. The single nucleotide polymorphisms associated with LD activity could be used in early generation selection for barley breeding.
authors.affiliation: Agronomy Department, Key Laboratory of Crop Germplasm Resource of Zhejiang Province, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Jin
authors.forename: Shengguan
authors.initials: S
authors.lastname: Cai
authors.forename: Lingzhen
authors.initials: L
authors.lastname: Ye
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Chen
authors.forename: Meixue
authors.initials: M
authors.lastname: Zhou
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhang
mesh_terms: D004791:Enzyme Inhibitors / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D001467:Hordeum / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics; D020641:Polymorphism, Single Nucleotide
chemical_list: D004791:Enzyme Inhibitors; D010940:Plant Proteins; D006026:Glycoside Hydrolases; C100181:pullulanase