title: Proteome atlas of human chromosome 8 and its multiple 8p deficiencies in tumorigenesis of the stomach, colon, and liver.
abstract: Chromosome 8, a medium-length euchromatic unit in humans that has an extraordinarily high mutation rate, can be detected not only in evolution but also in multiple mutant diseases, such as tumorigenesis, and further invasion/metastasis. The Chromosome-Centric Human Proteome Project of China systematically profiles the proteomes of three digestive organs (i.e., stomach, colon, and liver) and their corresponding carcinoma tissues/cell lines according to a chromosome organizational roadmap. By rigorous standards, we have identified 271 (38.7%), 330 (47.1%), and 325 (46.4%) of 701 chromosome 8-coded proteins from stomach, colon, and liver samples, respectively, in Swiss-Prot and observed a total coverage rate of up to 58.9% by 413 identified proteins. Using large-scale label-free proteome quantitation, we also found some 8p deficiencies, such as the presence of 8p21-p23 in tumorigenesis of the above-described digestive organs, which is in good agreement with previous reports. To our best knowledge, this is the first study to have verified these 8p deficiencies at the proteome level, complementing genome and transcriptome data.
authors.affiliation: Institutes of Biomedical Sciences and Department of Chemistry, Fudan University, Shanghai 200032, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Guoquan
authors.initials: G
authors.lastname: Yan
authors.forename: Linhui
authors.initials: L
authors.lastname: Zhai
authors.forename: Shaohang
authors.initials: S
authors.lastname: Xu
authors.forename: Huali
authors.initials: H
authors.lastname: Shen
authors.forename: Jun
authors.initials: J
authors.lastname: Yao
authors.forename: Feifei
authors.initials: F
authors.lastname: Wu
authors.forename: Liqi
authors.initials: L
authors.lastname: Xie
authors.forename: Hailin
authors.initials: H
authors.lastname: Tang
authors.forename: Hongxiu
authors.initials: H
authors.lastname: Yu
authors.forename: Mingqi
authors.initials: M
authors.lastname: Liu
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Yang
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
authors.forename: Chengpu
authors.initials: C
authors.lastname: Zhang
authors.forename: Liwei
authors.initials: L
authors.lastname: Li
authors.forename: Cheng
authors.initials: C
authors.lastname: Chang
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Songfeng
authors.initials: S
authors.lastname: Wu
authors.forename: Yunping
authors.initials: Y
authors.lastname: Zhu
authors.forename: Quanhui
authors.initials: Q
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Wen
authors.forename: Liang
authors.initials: L
authors.lastname: Lin
authors.forename: Yinzhu
authors.initials: Y
authors.lastname: Wang
authors.forename: Guiyan
authors.initials: G
authors.lastname: Zheng
authors.forename: Lanping
authors.initials: L
authors.lastname: Zhou
authors.forename: Haojie
authors.initials: H
authors.lastname: Lu
authors.forename: Siqi
authors.initials: S
authors.lastname: Liu
authors.forename: Fuchu
authors.initials: F
authors.lastname: He
authors.forename: Fan
authors.initials: F
authors.lastname: Zhong
mesh_terms: D002471:Cell Transformation, Neoplastic; D002872:Chromosome Deletion; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics / Q000378:metabolism; D003106:Colon / Q000378:metabolism / Q000473:pathology; D030562:Databases, Protein; D005753:Gastric Mucosa / Q000378:metabolism; D015894:Genome, Human; D016045:Human Genome Project; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology; D011506:Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism; D020543:Proteome; D013270:Stomach / Q000473:pathology
chemical_list: D011506:Proteins; D020543:Proteome