title: Function of cytochrome P450 enzymes RosC and RosD in the biosynthesis of rosamicin macrolide antibiotic produced by Micromonospora rosaria.
abstract: The cytochrome P450 enzyme-encoding genes rosC and rosD were cloned from the rosamicin biosynthetic gene cluster of Micromonospora rosaria IFO13697. The functions of RosC and RosD were demonstrated by gene disruption and complementation with M. rosaria and bioconversion of rosamicin biosynthetic intermediates with Escherichia coli expressing RosC and RosD. It is proposed that M. rosaria IFO13697 has two pathway branches that lead from the first desosaminyl rosamicin intermediate, 20-deoxo-20-dihydro-12,13-deepoxyrosamicin, to rosamicin.
authors.affiliation: Faculty of Pharmaceutical Sciences, Toho University, Funabashi, Chiba, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Iizaka
authors.forename: Noriko
authors.initials: N
authors.lastname: Higashi
authors.forename: Masanari
authors.initials: M
authors.lastname: Ishida
authors.forename: Reina
authors.initials: R
authors.lastname: Oiwa
authors.forename: Yumi
authors.initials: Y
authors.lastname: Ichikawa
authors.forename: Moeka
authors.initials: M
authors.lastname: Takeda
authors.forename: Yojiro
authors.initials: Y
authors.lastname: Anzai
authors.forename: Fumio
authors.initials: F
authors.lastname: Kato
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D017353:Gene Deletion; D005816:Genetic Complementation Test; D007933:Leucomycins / Q000096:biosynthesis*; D008847:Micromonospora / Q000201:enzymology* / Q000235:genetics*; D005810:Multigene Family
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D007933:Leucomycins; D003577:Cytochrome P-450 Enzyme System; C060286:rosaramicin