title: Simultaneous imaging of an enantiomer pair by electron paramagnetic resonance using isotopic nitrogen labeling.
abstract: This Article describes the simultaneous imaging of chiral nitroxyl radicals using electron paramagnetic resonance (EPR). Chiral nitroxyl radicals could be simultaneously visualized with the labeling of isotopic nitrogen. Chiral nitroxyl radicals, hydroxylmethyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl, were visualized using the method of simultaneous EPR imaging, which refers to the visualization of two kinds of molecules with unpaired electrons in a single image scan. EPR spectra of a racemic mixture of chiral nitroxyl radicals and those of the respective R and S configurations confirmed labeling by isotopic nitrogen. (1)H nuclear magnetic resonance (NMR) imaging and simultaneous imaging of solutions of chiral nitroxyl radicals were performed. The advantages and limitations of simultaneous imaging using EPR are also discussed. Simultaneous imaging with chiral-labeled nitroxyl radicals is a new application of EPR imaging and may be useful for biological studies involving biologically active chiral molecules.
authors.affiliation: Division of Bioengineering and Bioinformatics, Graduate School of Information Science and Technology, Hokkaido University, North 14, West 9, Sapporo, 060-0814, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Miyake
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Wang
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Amasaka
authors.forename: Kaori
authors.initials: K
authors.lastname: Itto
authors.forename: Shu
authors.initials: S
authors.lastname: Xu
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Arimoto
authors.forename: Hirotada
authors.initials: H
authors.lastname: Fujii
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hirata
mesh_terms: D003497:Cyclic N-Oxides / Q000138:chemical synthesis / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D007553:Isotope Labeling; D008968:Molecular Conformation; D009587:Nitrogen Isotopes; D009589:Nitrogen Oxides / Q000138:chemical synthesis / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D003497:Cyclic N-Oxides; D009587:Nitrogen Isotopes; D009589:Nitrogen Oxides; C584553:hydroxylmethyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl; C039900:nitroxyl