title: Distribution and compartmentalization of human circulating and tissue-resident memory T cell subsets.
abstract: Knowledge of human T cells derives chiefly from studies of peripheral blood, whereas their distribution and function in tissues remains largely unknown. Here, we present a unique analysis of human T cells in lymphoid and mucosal tissues obtained from individual organ donors, revealing tissue-intrinsic compartmentalization of naive, effector, and memory subsets conserved between diverse individuals. Effector memory CD4(+) T cells producing IL-2 predominated in mucosal tissues and accumulated as central memory subsets in lymphoid tissue, whereas CD8(+) T cells were maintained as naive subsets in lymphoid tissues and IFN-γ-producing effector memory CD8(+) T cells in mucosal sites. The T cell activation marker CD69 was constitutively expressed by memory T cells in all tissues, distinguishing them from circulating subsets, with mucosal memory T cells exhibiting additional distinct phenotypic and functional properties. Our results provide an assessment of human T cell compartmentalization as a new baseline for understanding human adaptive immunity.
authors.affiliation: Columbia Center for Translational Immunology, Columbia University Medical Center, New York, NY 10032, USA.
authors.forename: Taheri
authors.initials: T
authors.lastname: Sathaliyawala
authors.forename: Masaru
authors.initials: M
authors.lastname: Kubota
authors.forename: Naomi
authors.initials: N
authors.lastname: Yudanin
authors.forename: Damian
authors.initials: D
authors.lastname: Turner
authors.forename: Philip
authors.initials: P
authors.lastname: Camp
authors.forename: Joseph J C
authors.initials: JJ
authors.lastname: Thome
authors.forename: Kara L
authors.initials: KL
authors.lastname: Bickham
authors.forename: Harvey
authors.initials: H
authors.lastname: Lerner
authors.forename: Michael
authors.initials: M
authors.lastname: Goldstein
authors.forename: Megan
authors.initials: M
authors.lastname: Sykes
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Kato
authors.forename: Donna L
authors.initials: DL
authors.lastname: Farber
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D015703:Antigens, CD / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D005260:Female; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology*; D016130:Immunophenotyping; D039001:Integrin alpha Chains / Q000378:metabolism; D037181:Lectins, C-Type / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009092:Mucous Membrane / Q000276:immunology; D009928:Organ Specificity / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D014019:Tissue Donors; D055815:Young Adult; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D039001:Integrin alpha Chains; D037181:Lectins, C-Type; D000099192:CD69 Antigens; C091367:alpha E integrins