title: Comparative study of the effects of recombinant human epidermal growth factor and basic fibroblast growth factor on corneal epithelial wound healing and neovascularization in vivo and in vitro.
abstract: OBJECTIVE
  This study was undertaken to investigate the effects of recombinant human epidermal growth factor (rhEGF) and basic fibroblast growth factor (bFGF) on corneal wound healing and neovascularization (CNV).
  
  
  METHODS
  The positive effects of 10 ng/ml rhEGF and bFGF on the proliferation of corneal epithelial cells (SD-HCEC1s), rabbit keratocyte cells (RKCs) and human umbilical vein endothelial cells (HUVECs) as well as the effects on the migration capacity on HUVECs were observed. An animal central corneal wound and CNV model was established in rabbits. One eye of each group was chosen randomly for topical administration of rhEGF, bFGF or normal saline, and variability in the area of corneal epithelial wound healing and CNV was observed.
  
  
  RESULTS
  The optimal concentration of rhEGF and bFGF for the proliferation of corneal epithelial cells was 10 ng/ml. The promotive effect of 10 ng/ml rhEGF on the proliferation of RKCs and HUVECs was less than that of 10 ng/ml bFGF. In the animal experiment, the healing rate of the corneal epithelium in the rhEGF group was better than in the other groups on day 1. On day 3, the healing rates of the 3 groups were nearly equal. The CNV area in the rhEGF group was less than that of the bFGF group.
  
  
  CONCLUSIONS
  rhEGF and bFGF both had promotive effects on corneal epithelial wound healing, but rhEGF had a weaker promotive effect on CNV than bFGF. With long-term application of growth factor drugs, rhEGF is suggested for lessening the growth of CNV.
authors.affiliation: Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou, PR China.
authors.forename: Limeng
authors.initials: L
authors.lastname: Yan
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Zhichong
authors.initials: Z
authors.lastname: Wang
authors.forename: Chaoyang
authors.initials: C
authors.lastname: Li
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Lu
authors.forename: Hucheng
authors.initials: H
authors.lastname: Duan
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
authors.forename: Xiaoran
authors.initials: X
authors.lastname: Wang
authors.forename: Pengxia
authors.initials: P
authors.lastname: Wan
authors.forename: Yiyue
authors.initials: Y
authors.lastname: Song
authors.forename: Jing
authors.initials: J
authors.lastname: Tang
authors.forename: Yu
authors.initials: Y
authors.lastname: Han
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects; D019573:Epithelium, Corneal / Q000187:drug effects* / Q000293:injuries; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects; D009389:Neovascularization, Pathologic / Q000188:drug therapy*; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology; D014471:Umbilical Veins / Q000166:cytology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D011994:Recombinant Proteins; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor