title: E-ADA activity in serum of lambs experimentally infected with Haemonchus contortus.
abstract: The aim of this study was to evaluate adenosine deaminase (E-ADA) activity in sera of lambs experimentally infected with Haemonchus contortus. We used 12 lambs divided into 2 groups; Group A had 5 healthy, non-infected animals (control) and Group B had 7 healthy animals infected with H. contortus . Lambs were infected orally with 500 larvae (L3) per animal every 2 days, for a period of 20 days, and later the infection was confirmed by examination of feces (eggs per gram [EPG] via fecal egg count). Blood collection was performed at days 0, 20, 40, 60, and 80 post-infection (PI) for analysis of E-ADA activity. Animals in Group A showed negative EPG throughout the experiment unlike those from Group B that had elevated EPG counts. E-ADA activity was reduced in the serum of animals infected with H. contortus when compared to non-infected controls at days 20, 40, 60, and 80 PI. Therefore, it is concluded that infection with H. contortus influences the E-ADA activity in lambs.
authors.affiliation: Department of Animal Science, Universidade do Estado de Santa Catarina, Brazil. aleksandro_ss@yahoo.com.br
authors.forename: Aleksandro S
authors.initials: AS
authors.lastname: Da Silva
authors.forename: Guilherme C
authors.initials: GC
authors.lastname: Fausto
authors.forename: Thirssa H
authors.initials: TH
authors.lastname: Grando
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Cadore
authors.forename: Victor C
authors.initials: VC
authors.lastname: Pimentel
authors.forename: Jeandre A
authors.initials: JA
authors.lastname: Jaques
authors.forename: Maria R C
authors.initials: MR
authors.lastname: Schetinger
authors.forename: Silvia G
authors.initials: SG
authors.lastname: Monteiro
authors.forename: Marta L R
authors.initials: ML
authors.lastname: Leal
mesh_terms: D000241:Adenosine / Q000378:metabolism*; D000243:Adenosine Deaminase / Q000097:blood*; D000818:Animals; D016022:Case-Control Studies; D005243:Feces / Q000469:parasitology; D006188:Haemonchiasis / Q000097:blood / Q000201:enzymology / Q000662:veterinary*; D006189:Haemonchus / Q000201:enzymology*; D008297:Male; D010270:Parasite Egg Count / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000097:blood / Q000201:enzymology* / Q000469:parasitology
chemical_list: D000243:Adenosine Deaminase; D000241:Adenosine