title: Microsomal triglyceride transfer protein gene -493G/T polymorphism and its association with serum lipid levels in Bama Zhuang long-living families in China.
abstract: BACKGROUND
  The -493G/T polymorphism in the microsomal triglyceride transfer protein (MTP) gene is associated with lower serum low-density lipoprotein cholesterol (LDL-C) and triglyceride (TG) levels and longevity in several populations, but the results are inconsistent in different racial/ethnic groups. The current study was to investigate the plausible association of MTP -493G/T polymorphism with serum lipid levels and longevity in Zhuang long-lived families residing in Bama area, a famous home of longevity in Guangxi, China.
  
  
  METHODS
  The MTP -493G/T was genotyped by PCR-restriction fragment length polymorphism in 391 Bama Zhuang long-lived families (BLF, n = 1467, age 56.60 ± 29.43 years) and four control groups recruited from Bama and out-of-Bama area with or without a familial history of exceptional longevity: Bama non-long-lived families (BNLF, n = 586, age 44.81 ± 26.83 years), Bama non-Zhuang long-lived families (BNZLF, n = 444, age 52.09 ± 31.91 years), Pingguo long-lived families (PLF, n = 658, age 50.83 ± 30.30 years), and Pingguo non-long-lived families (PNLF, n = 539, age 38.74 ± 24.69 years). Correlation analyses between genotypes and serum lipid levels and longevity were then performed.
  
  
  RESULTS
  No particularly favorable lipoprotein and clinical phenotypes were seen in BLF as compared to general families in the same area. Instead, the levels of total cholesterol (TC), TG, LDL-C, and the prevalence of dyslipidemia were significantly higher in the three Bama families as compared to the two non-Bama families (P < 0.01 for all). There were no differences in the allelic and genotypic frequencies among the tested cohorts (P > 0.05 for all), but the TT genotype tended to enrich in the three long-lived cohorts from both areas. In addition, the individuals harboring TT genotype exhibited lower LDL-C and TC levels in the overall populations and Bama populations with a region- and sex-specific pattern. Multiple linear regression analyses unraveled that LDL-C levels were correlated with genotypes in Bama combined population, BNLF, and the total population (P < 0.05 for each) but not in Pingguo populations; TC and HDL-C levels were correlated with genotypes in Bama combined population and BLF, respectively (P < 0.05 for each).
  
  
  CONCLUSIONS
  MTP -493G/T polymorphism may play an important role in fashioning the serum lipid profiles of Bama populations, despite no direct association between MTP -493G/T and longevity was detected.
authors.affiliation: Department of Cardiology, Institute of Cardiovascular Diseases, the First Affiliated Hospital, Guangxi Medical University, 22 Shuangyong Road, Nanning, Guangxi, 530021, People's Republic of China.
authors.forename: Shang-Ling
authors.initials: SL
authors.lastname: Pan
authors.forename: Xiao-Qiu
authors.initials: XQ
authors.lastname: Luo
authors.forename: Ze-Ping
authors.initials: ZP
authors.lastname: Lu
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Lu
authors.forename: Huan
authors.initials: H
authors.lastname: Luo
authors.forename: Cheng-Wu
authors.initials: CW
authors.lastname: Liu
authors.forename: Cai-You
authors.initials: CY
authors.lastname: Hu
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Du
authors.forename: Zhen
authors.initials: Z
authors.lastname: Song
authors.forename: Guo-Fang
authors.initials: GF
authors.lastname: Pang
authors.forename: Hua-Yu
authors.initials: HY
authors.lastname: Wu
authors.forename: Jin-Bo
authors.initials: JB
authors.lastname: Huang
authors.forename: Jun-Hua
authors.initials: JH
authors.lastname: Peng
authors.forename: Rui-Xing
authors.initials: RX
authors.lastname: Yin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001055:Apolipoproteins B / Q000097:blood / Q000235:genetics; D044466:Asian People / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics*; D002681:China; D008078:Cholesterol, LDL / Q000097:blood / Q000235:genetics; D050171:Dyslipidemias / Q000097:blood / Q000235:genetics; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008136:Longevity / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D014280:Triglycerides / Q000097:blood / Q000235:genetics
chemical_list: D001055:Apolipoproteins B; D002352:Carrier Proteins; D008078:Cholesterol, LDL; D014280:Triglycerides; C106092:microsomal triglyceride transfer protein