title: Biosynthesis of 3-hydroxypropionic acid from glycerol in recombinant Escherichia coli expressing Lactobacillus brevis dhaB and dhaR gene clusters and E. coli K-12 aldH.
abstract: 3-Hydroxypropionic acid (3-HP) is a value-added chemical for polymer synthesis. For biosynthesis of 3-HP from glycerol, two dhaB and dhaR clusters encoding glycerol dehydratase and its reactivating factor, respectively, were cloned from Lactobacillus brevis KCTC33069 and expressed in Escherichia coli. Coexpression of dhaB and dhaR allowed the recombinant E. coli to convert glycerol to 3-hydroxypropionaldehyde, an intermediate of 3-HP biosynthesis. To produce 3-HP from glycerol, fed-batch fermentation with a two-step feeding strategy was designed to separate the cell growth from the 3-HP production stages. Finally, E. coli JHS00947 expressing L .brevis dhaB and dhaR, and E. coli aldH produced 14.3g/L 3-HP with 0.26 g/L-h productivity, which were 14.6 and 8.53 times higher than those of the batch culture. In conclusion, overexpression of L. brevis dhaB and dhaR clusters and E. coli aldH, and implementation of the two-step feeding strategy enabled recombinant E. coli to convert glycerol to 3-HP efficiently.
authors.affiliation: Interdisciplinary Program for Bioengineering, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Suryang
authors.initials: S
authors.lastname: Kwak
authors.forename: Yong-Cheol
authors.initials: YC
authors.lastname: Park
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Seo
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D000595:Amino Acid Sequence; D061249:Batch Cell Culture Techniques; D048168:Escherichia coli K12 / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D005798:Genes, Bacterial / Q000235:genetics*; D005990:Glycerol / Q000378:metabolism*; D006836:Hydro-Lyases / Q000378:metabolism; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D019344:Lactic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis; D052196:Levilactobacillus brevis / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics*; D013696:Temperature
chemical_list: D019344:Lactic Acid; D007544:Isopropyl Thiogalactoside; C031601:hydracrylic acid; D000444:Aldehyde Dehydrogenase; D006836:Hydro-Lyases; C020671:glycerol dehydratase; D005990:Glycerol