title: [Correlation between reactive oxygen species of cryopreserved peripheral blood mononuclear cells and expression of homing adhesion molecules on peripheral blood hematopoietic stem/progenitor cells].
abstract: This study was purposed to detect the level of reactive oxygen species (ROS) in cryopreserved peripheral blood mononuclear cells (PBMNC) and the expression of homing molecules on peripheral blood hematopoietic stem/progenitor cells, and to analyze the correlation between ROS level and the expression of homing molecules. The survival percentage of PBMNC was determined by trypan blue exclusion rate. The expression of antigens on peripheral blood hematopoietic stem/progenitor cells, such as CD34, CD133 and some related homing molecules (VLA4, CXCR4 and CD44) were detected by flow cytometry. The correlation of the ROS levels with the expression of homing molecules was evaluated by the correlation coefficient. The results showed that the survival rate of PBMNC was gradually reduced along with prolongation of cryopreserved time, and there was a significant difference in the survival percentage of cryopreserved PBMNC between experimental group frozen for 3, 6, 9, 12 months and control group (P < 0.05). The expression of marked antigen CD34 and CD133, and homing adhesive molecules of CD44, VLA4 and CXCR4 declined with the extension of frozen time, and there was significant statistical difference (P < 0.05) between experimental group cryopreserved for a year and control group. The level of ROS in PBMNC gradually increased along with the prolongation of cryopreserved time, and there was significant statistical difference (P < 0.05) between experimental group freezing-stored for 6, 9 and 12 months and control group. The correlation coefficients of ROS level and expression percentage CD34(+) VLA4(+), CD34(+) CXCR4(+), CD34(+) CD44(+) PBMNC were -0.50, -0.457, -0.465, respectively. It is concluded that ROS level in PBMNC increases significantly with the prolonged cryopreservation time, especially for more than 6 months. There is a significant negative correlation between the ROS level of PBMNC and the expression rate of homing molecules. Higher level of ROS may be one of the reasons for the reduction of homing capability in peripheral blood stem/progenitor cells after cryopreservation.
authors.affiliation: Department of Transfusion, the First Hospital of China Medical University, Shenyang110001, Liaoning Province, China.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Jin
authors.forename: Huan-Ming
authors.initials: HM
authors.lastname: Xu
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Hao
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D015925:Cryopreservation; D006023:Glycoproteins / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D019718:Receptors, CXCR4 / Q000378:metabolism
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; C497483:CD44 protein, human; C527276:CXCR4 protein, human; D006023:Glycoproteins; D018960:Hyaluronan Receptors; C000606023:PROM1 protein, human; D010455:Peptides; D017382:Reactive Oxygen Species; D019718:Receptors, CXCR4