title: Optimization of synthesis, characterization and cytotoxic activity of Seleno-Capparis spionosa L. polysaccharide.
abstract: In this study, an experiment was designed to optimize the synthesis of  seleno-Capparis spionosa L. polysaccharide (Se-CSPS) by response surface methodology. Three independent variables (reaction time, reaction temperature and ratio of Na(2)SeO(3) to CSPS) were tested. Furthermore, the thermal stability, particle size, shape and cytotoxic activity of Se-CSPS in vitro were investigated. The optimum reaction conditions were obtained shown as follows: reaction time 7.5 h, reaction temperature 71 °C, and ratio of Na(2)SeO(3) to CSPS 0.9 g/g. Under these conditions, the Se content in Se-CSPS reached 5.547 mg/g, which was close to the predicted value (5.518 mg/g) by the model. The thermal stability, particle size and shape of Se-CSPS were significantly different from those of CSPS. Additionally, a MTT assay indicated that the Se-CSPS could inhibit the proliferation of human gastric cancer SGC-7901 cells in a dose-dependent manner.
authors.affiliation: Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China. jyb@hrbcu.edu.cn.
authors.forename: Yu-Bin
authors.initials: YB
authors.lastname: Ji
authors.forename: Fang
authors.initials: F
authors.lastname: Dong
authors.forename: Lang
authors.initials: L
authors.lastname: Lang
authors.forename: Ling-Wen
authors.initials: LW
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Miao
authors.forename: Zhen-Feng
authors.initials: ZF
authors.lastname: Liu
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Jin
authors.forename: Ying
authors.initials: Y
authors.lastname: Hao
mesh_terms: D031484:Capparis / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003603:Cytotoxins / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D011134:Polysaccharides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D012643:Selenium / Q000737:chemistry / Q000494:pharmacology
chemical_list: D003603:Cytotoxins; D011134:Polysaccharides; D012643:Selenium