title: Studies on cytotoxic triterpene saponins from the leaves of Aralia elata.
abstract: Aralia elata has long been used as a tonic, anticancer and antidiabetic agent in China and Japan, and is widely consumed as food. Phytochemical investigation of the leaves of A. elata has led to the isolation of four new compounds, 3-O-[β-D-glucopyranosyl(1 → 3)-β-D-glucopyranosyl] echinocystic acid 28-O-β-D-glucopyranosyl ester (congmuyenoside I, 1), 3-O-[β-D-glucopyranosyl(1 → 2)-β-D-glucopyranosyl] hederagenin 28-O-β-D-glucopyranosyl ester (congmuyenoside II, 2), 3-O-{[β-D-glucopyranosyl(1 → 2)]-[β-D-glucopyranosyl(1 → 3)-β-D-glucopyranosyl(1 → 3)]-β-D-glucopyranosyl} echinocystic acid 28-O-β-D-glucopyranosyl ester (congmuyenoside III, 3) and 3-O-β-D-glucopyranosyl caulophyllogenin 28-O-β-D-glucopyranosyl ester (congmuyenoside IV, 4), and eight known triterpene saponins (5-12). The structural determination was accomplished with spectroscopic analysis, in particularly (13)C NMR, 2D NMR and HR-ESI-MS techniques. In addition, compounds 5–10 were found for the first time in the genus Aralia. Compounds 1-12 were tested for their inhibition of the growth of HL60, A549 and DU145 cancer cells. In addition, compound 8 showed significant cytotoxic activities against HL60, A549 and DU145 cancer cells with IC(50) values of 15.62, 11.25 and 7.59 μM, respectively.
authors.affiliation: School of Traditional Chinese Materia Medica, Key Laboratory of Structure-Based Drug Design & Discovery, Shenyang Pharmaceutical University, Shenyang 110016, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.forename: Lingzhi
authors.initials: L
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.forename: Chong
authors.initials: C
authors.lastname: Hu
authors.forename: Shaojiang
authors.initials: S
authors.lastname: Song
mesh_terms: D027983:Aralia / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000633:toxicity*; D018515:Plant Leaves / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000633:toxicity*; D014315:Triterpenes / Q000737:chemistry / Q000633:toxicity*
chemical_list: D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes