title: Highly enantioselective oxidation of α-hydroxyacids bearing a substituent with an aryl group: co-production of optically active α-hydroxyacids and α-ketoacids.
abstract: A novel and simple methodology for co-obtaining of enantiomerically pure α-hydroxyacids and α-ketoacids was developed by enantioselective oxidation of α-hydroxyacids bearing a substituent with an aryl group using α-hydroxyacid dehydrogenase (α-HADH). A high-throughput method was firstly established for screening of enantioselective α-HADHs. Sinorhizobium sp. ZJB1 101 with high activity and excellent enantioselectivity of α-HADH for oxidation of α-hydroxyacids bearing a substituent with an aryl group was isolated and identified. This strain has potential for co-production of (R)-α-hydroxyacids and α-ketoacids in near theoretical yields, while no consecutive oxidation of α-ketoacids was observed. The green conversion system appears promising for potential applications in industry.
authors.affiliation: Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China.
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Xue
authors.forename: Ya-Qin
authors.initials: YQ
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Wang
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Liu
authors.forename: Shu-Ping
authors.initials: SP
authors.lastname: Zou
authors.forename: Yu-Guo
authors.initials: YG
authors.lastname: Zheng
authors.forename: Yin-Chu
authors.initials: YC
authors.lastname: Shen
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D002384:Catalysis; D057166:High-Throughput Screening Assays / Q000379:methods*; D006880:Hydroxy Acids / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D007651:Keto Acids / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D015394:Molecular Structure; D010084:Oxidation-Reduction; D020579:Sinorhizobium / Q000201:enzymology*
chemical_list: D006880:Hydroxy Acids; D007651:Keto Acids; D000429:Alcohol Oxidoreductases; C021379:D-2-hydroxyacid dehydrogenase