title: Tmprss3 loss of function impairs cochlear inner hair cell Kcnma1 channel membrane expression.
abstract: Before acquiring their mature state, cochlear hair cells undergo a series of changes in expression of ion channels. How this complex mechanism is achieved is not fully understood. Tmprss3, a type II serine protease expressed in hair cells, is required for their proper functioning at the onset of hearing. To unravel the role of Tmprss3 in the acquisition of mature K(+) currents, we compared their function by patch-clamp technique in wild-type Tmprss3(WT) and Tmprss3(Y260X)-mutant mice. Interestingly, only outward K(+) currents were altered in Tmprss3(Y260X)-mutant mice. To determine by which mechanism this occurred, we compared the protein network of Tmprss3(WT) and Tmprss3(Y260X)-mutant mice using proteomic analysis. This led to the identification of a pathway related to potassium Kcnma1 channels. This pathway was validated by immunohistochemistry, focusing on the most downregulated protein that was identified as a cochlear Kcnma1-associated protein, APOA1. Finally, we show that, in contrast to Tmprss3(WT), Kcnma1 channels were absent at the neck of inner hair cells (IHCs) in Tmprss3(Y260X)-mutant mice. In conclusion, our data suggest that lack of Tmprss3 leads to a decrease in Kcnma1 potassium channels expression in (IHCs).
authors.affiliation: SysDiag UMR 3145 CNRS/Bio-Rad, Cap Delta/Parc Euromédecine, 1682 rue de la Valsière, Cedex 4,Montpellier, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Molina
authors.forename: Lydie
authors.initials: L
authors.lastname: Fasquelle
authors.forename: Régis
authors.initials: R
authors.lastname: Nouvian
authors.forename: Nicolas
authors.initials: N
authors.lastname: Salvetat
authors.forename: Hamish S
authors.initials: HS
authors.lastname: Scott
authors.forename: Michel
authors.initials: M
authors.lastname: Guipponi
authors.forename: Franck
authors.initials: F
authors.lastname: Molina
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Puel
authors.forename: Benjamin
authors.initials: B
authors.lastname: Delprat
mesh_terms: D000818:Animals; D016632:Apolipoprotein A-I / Q000235:genetics / Q000378:metabolism; D003051:Cochlea / Q000166:cytology; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D015870:Gene Expression; D006199:Hair Cells, Auditory, Inner / Q000378:metabolism*; D006801:Humans; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits / Q000235:genetics / Q000378:metabolism*; D008564:Membrane Potentials; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D051379:Mice; D008822:Mice, Transgenic; D020125:Mutation, Missense; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000378:metabolism; D021381:Protein Transport; D020543:Proteome / Q000378:metabolism; D057057:Serine Proteases / Q000235:genetics* / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D016632:Apolipoprotein A-I; C465449:Kcnma1 protein, mouse; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; D008565:Membrane Proteins; D020543:Proteome; D057057:Serine Proteases; C559262:Tmprss3 protein, mouse; D011188:Potassium