title: Characterization of Cdc2 kinase in the red claw crayfish (Cherax quadricarinatus): evidence for its role in regulating oogenesis.
abstract: Cdc2 kinase is a catalytic subunit of the maturation-promoting factor (MPF), a central factor for inducing the meiotic maturation of oocytes. MPF has been studied in a wide variety of animal species; however, its expression in crustaceans is poorly characterized. In this study, a complete cDNA sequence of Cdc2 kinase was cloned from the red claw crayfish, Cherax quadricarinatus, and its spatiotemporal expression profiles were analyzed. The Cdc2 cDNA (1,769 bp) encodes for a 299 amino acid protein with a calculated molecular weight of 34.7 kDa. Quantitative real-time PCR demonstrated that Cdc2 mRNA was expressed mainly in the ovary tissue and the expression decreased as the ovaries developed. Immunohistochemistry analysis revealed that the Cdc2 protein relocated from the cytoplasm to the nucleus during oogenesis. These findings suggest that Cdc2 kinase may play an important role in the gametogenesis and gonad development in C. quadricarinatus.
authors.affiliation: Life Science College, East China Normal University, Shanghai 200062, China.
authors.forename: Lan-Mei
authors.initials: LM
authors.lastname: Wang
authors.forename: Di
authors.initials: D
authors.lastname: Zuo
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Lv
authors.forename: Dan-Li
authors.initials: DL
authors.lastname: Wang
authors.forename: A-Jing
authors.initials: AJ
authors.lastname: Liu
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D003400:Astacoidea / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D016203:CDC2 Protein Kinase / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D005260:Female; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D008969:Molecular Sequence Data; D009866:Oogenesis; D009928:Organ Specificity; D010053:Ovary / Q000166:cytology / Q000201:enzymology / Q000254:growth & development; D010802:Phylogeny; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D060829:Arthropod Proteins; D021122:Protein Subunits; D016203:CDC2 Protein Kinase