title: [Effect of total flavones of Rhododendra simsii on the activation of ERK induced by cerebral ischemia-reperfusion in rats].
abstract: OBJECTIVE
  To study the effect of total flavones of Rhododendron simsii (TFR) on the activation of ERK induced by cerebral ischemia-reperfusion in rats and its possible mechanism.
  
  
  METHODS
  Cerebral ischemia-reperfusion were induced by 4-vessel occlusion in rats. Changes in electroencephalogram were recorded before and 10 min after ischemia and 5,10,15,30,45 and 60 min after reperfusion. The activation of ERK1/2 and p-ERK1/2 of cerebral cortex and hippocampus was investigated by western blot.
  
  
  RESULTS
  Compared with sham-operate group, EEG amplitude decreased significantly after ischemia and recovered after reperfusion in NS control and at 45 min and 60 min, EEG amplitude of TFR (15, 30, 60 mg/kg) groups was significantly higher than that of NS control (P < 0.05 or P < 0.01). TFR (30, 60 mg/kg) could increase the activation of ERK1/2 after cerebral ischmia-reperfusion.
  
  
  CONCLUSIONS
  TFR (15, 30, 60 mg/kg) could significantly promote the recovery of EEG and the mechanism might be associated with the increase of the activation of ERK1/2 after cerebral ischemia-reperfusion.
authors.affiliation: Department of Pharmacology, Anhui Medical University, Hefei 230032, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.forename: Zhi-Wu
authors.initials: ZW
authors.lastname: Chen
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002545:Brain Ischemia / Q000378:metabolism / Q000517:prevention & control*; D004195:Disease Models, Animal; D004569:Electroencephalography / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D047309:Flavones / Q000494:pharmacology*; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D008517:Phytotherapy; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D029793:Rhododendron / Q000737:chemistry*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D047309:Flavones; D018696:Neuroprotective Agents; D048049:Extracellular Signal-Regulated MAP Kinases