title: Missense mutations linked to friedreich ataxia have different but synergistic effects on mitochondrial frataxin isoforms.
abstract: Friedreich ataxia is an early-onset multisystemic disease linked to a variety of molecular defects in the nuclear gene FRDA. This gene normally encodes the iron-binding protein frataxin (FXN), which is critical for mitochondrial iron metabolism, global cellular iron homeostasis, and antioxidant protection. In most Friedreich ataxia patients, a large GAA-repeat expansion is present within the first intron of both FRDA alleles, that results in transcriptional silencing ultimately leading to insufficient levels of FXN protein in the mitochondrial matrix and probably other cellular compartments. The lack of FXN in turn impairs incorporation of iron into iron-sulfur cluster and heme cofactors, causing widespread enzymatic deficits and oxidative damage catalyzed by excess labile iron. In a minority of patients, a typical GAA expansion is present in only one FRDA allele, whereas a missense mutation is found in the other allele. Although it is known that the disease course for these patients can be as severe as for patients with two expanded FRDA alleles, the underlying pathophysiological mechanisms are not understood. Human cells normally contain two major mitochondrial isoforms of FXN (FXN(42-210) and FXN(81-210)) that have different biochemical properties and functional roles. Using cell-free systems and different cellular models, we show that two of the most clinically severe FXN point mutations, I154F and W155R, have unique direct and indirect effects on the stability, biogenesis, or catalytic activity of FXN(42-210) and FXN(81-210) under physiological conditions. Our data indicate that frataxin point mutations have complex biochemical effects that synergistically contribute to the pathophysiology of Friedreich ataxia.
authors.affiliation: Department of Pediatric and Adolescent Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA.
authors.forename: Hongqiao
authors.initials: H
authors.lastname: Li
authors.forename: Oleksandr
authors.initials: O
authors.lastname: Gakh
authors.forename: Douglas Y
authors.initials: DY
authors.lastname: Smith
authors.forename: Wasantha K
authors.initials: WK
authors.lastname: Ranatunga
authors.forename: Grazia
authors.initials: G
authors.lastname: Isaya
mesh_terms: D000483:Alleles; D019943:Amino Acid Substitution; D000818:Animals; D019556:COS Cells; D045744:Cell Line, Tumor; D002474:Cell-Free System; D002522:Chlorocebus aethiops; D005621:Friedreich Ataxia / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D033862:Iron-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D020125:Mutation, Missense; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D019680:Trinucleotide Repeat Expansion; D000096923:Frataxin
chemical_list: D033862:Iron-Binding Proteins; D024101:Mitochondrial Proteins; D020033:Protein Isoforms; D000096923:Frataxin