title: Butyric acid production from sugarcane bagasse hydrolysate by Clostridium tyrobutyricum immobilized in a fibrous-bed bioreactor.
abstract: A fermentation process using Clostridium tyrobutyricum immobilized in a fibrous-bed bioreactor (FBB) was developed for butyric acid production from sugarcane bagasse (SCB) hydrolysate. SCB was first treated with dilute acid and then hydrolyzed with cellulases. The hydrolysate containing glucose and xylose was used as carbon source for the fermentation without detoxification. The bacterium was able to grow at a specific growth rate of ∼0.06 h(-1) in media containing 15-20% (w/v) SCB in serum bottles. In batch cultures in the FBB, both glucose and xylose in the SCB hydrolysate were simultaneously converted to butyrate with a high yield (0.45-0.54 g/gsugar) and productivity (0.48-0.60 g/Lh). A final butyrate concentration of 20.9 g/L was obtained in a fed-batch culture, with an overall productivity of 0.51 g/Lh and butyrate yield of 0.48 g/g sugar consumed. This work demonstrated the feasibility of using SCB as a low-cost feedstock to produce butyric acid.
authors.affiliation: State Key Laboratory of Pulp and Paper Engineering, School of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, PR China; William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, USA. fewd304@scut.edu.cn
authors.forename: Dong
authors.initials: D
authors.lastname: Wei
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Liu
authors.forename: Shang-Tian
authors.initials: ST
authors.lastname: Yang
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods*; D019149:Bioreactors / Q000382:microbiology*; D020148:Butyric Acid / Q000302:isolation & purification / Q000378:metabolism*; D018914:Cells, Immobilized; D002482:Cellulose / Q000378:metabolism*; D048014:Clostridium tyrobutyricum / Q000378:metabolism*; D006868:Hydrolysis; D031786:Saccharum / Q000382:microbiology*
chemical_list: D020148:Butyric Acid; D002482:Cellulose; C027433:bagasse