title: Antioxidant and immunomodulatory activity of selenium exopolysaccharide produced by Lactococcus lactis subsp. lactis.
abstract: Exopolysaccharide (EPS) was isolated and purified from Lactococcus lactis subsp. Lactis culture broth. Selenium chloride oxide (SeCl(2)O) was added to the EPS to synthesize selenium-exopolysaccharide (Se-EPS). The in vitro and in vivo antioxidant and in vivo immunomodulatory activity of EPS and Se-EPS were compared. EPS and Se-EPS scavenged superoxide anions and hydroxyl radicals. They also increased catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, while decreasing malondialdehyde (MDA) levels in serum and in the livers of mice. Se-EPS showed stronger in vitro and in vivo antioxidant activity than were shown by EPS. The in vivo immunoenhancement activity of EPS and Se-EPS induced by cyclophosphamide (CY) treatment in immunosuppressed mice was researched. EPS and Se-EPS treatments increased macrophage phagocytosis, spleen and thymus indices and haemolytic complement activity (HC(50)). Se-EPS showed stronger immunomodulatory activity than did EPS.
authors.affiliation: Department of Food Science and Technology, Jinling College, Nanjing Normal University, Nanjing 210097, China.
authors.forename: Yuxing
authors.initials: Y
authors.lastname: Guo
authors.forename: Daodong
authors.initials: D
authors.lastname: Pan
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Yangying
authors.initials: Y
authors.lastname: Sun
authors.forename: Xiaoqun
authors.initials: X
authors.lastname: Zeng
authors.forename: Bingxiang
authors.initials: B
authors.lastname: Yan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D007155:Immunologic Factors / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D013294:Lactococcus lactis / Q000737:chemistry* / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010587:Phagocytosis / Q000187:drug effects; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D012643:Selenium / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D007155:Immunologic Factors; D011135:Polysaccharides, Bacterial; D012643:Selenium