title: Biochemical characterisation of Alaska pollock, Pacific whiting, and threadfin bream surimi as affected by comminution conditions.
abstract: Salt-soluble protein, surface reactive sulfhydryl content, and surface hydrophobicity of Alaska pollock, Pacific whiting, and threadfin bream surimi were characterised, as affected by various comminution conditions. Chopping time/temperatures were explored in consideration of their habitat temperatures. Salt-soluble protein (SSP) significantly decreased when chopping time was extended. Corresponding to our follow-up study, no relationship between SSP and gel texture was found. Surface hydrophobicity was inversely proportional to SSP concentration, indicating the unfolding of protein upon comminution. Alaska pollock surimi demonstrated aggregation during chopping at 10 and 20 °C, based on the surface hydrophobicity. Surface reactive sulfhydryl (SRSH) contents of the three fish species behaved differently. The SH groups were oxidized to disulphide bonds when higher chopping temperature was applied. As a result, increased SRSH content was not observed in Alaska pollock (10 and 20 °C chopping) and threadfin bream paste (25 and 30 °C chopping).
authors.affiliation: OSU Seafood Research and Education Center, 2001 Marine Dr. #253, Astoria, OR 97103, USA.
authors.forename: Samanan
authors.initials: S
authors.lastname: Poowakanjana
authors.forename: Jae W
authors.initials: JW
authors.lastname: Park
mesh_terms: D000413:Alaska; D000818:Animals; D029941:Fish Proteins / Q000737:chemistry; D005511:Food Handling / Q000379:methods*; D048388:Gadiformes; D010473:Perciformes; D017747:Seafood / Q000032:analysis*; D013696:Temperature
chemical_list: D029941:Fish Proteins