title: Application of a yeast estrogen reporter system for screening zearalenone degrading microbes.
abstract: The aim of this study was to screen microbes for their zearalenone degrading potential and to select microbes whose activities do not create toxic or endocrine disrupting metabolites. Bioluminescent bioreporters (Saccharomyces cerevisiae BLYES and BLYR) were successfully used to monitor toxin degradation; the results of zearalenone biodegradation experiments were confirmed by parallel chemical analysis (HPLC-FLD) and immunoanalytical (ELISA) tests. Using the BLYES/BLYR bioreporters, the most appropriate microbes (ones that produced minimal toxic products and products with lower estrogenic potential) could be selected. The most promising strains belong to Streptomyces and Rhodococcus genera. Our findings demonstrate the benefit of using biological tests beside the analytical method, since bioreporters were able to monitor the samples for toxicity and estrogenic potential even after substantial degradation. We conclude that the BLYES/BLYR bioreporter system is a cost effective, fast and reliable tool for screening zearalenone-degrading microbes.
authors.affiliation: Department of Environmental Protection & Safety, Szent István University, 1 Páter K St, Gödöllő 2100, Hungary.
authors.forename: Csilla
authors.initials: C
authors.lastname: Krifaton
authors.forename: Balázs
authors.initials: B
authors.lastname: Kriszt
authors.forename: Anita
authors.initials: A
authors.lastname: Risa
authors.forename: Sándor
authors.initials: S
authors.lastname: Szoboszlay
authors.forename: Mátyás
authors.initials: M
authors.lastname: Cserháti
authors.forename: Péter
authors.initials: P
authors.lastname: Harkai
authors.forename: Melanie
authors.initials: M
authors.lastname: Eldridge
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: József
authors.initials: J
authors.lastname: Kukolya
mesh_terms: D001681:Biological Assay; D004785:Environmental Pollutants / Q000737:chemistry / Q000378:metabolism*; D004967:Estrogens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017930:Genes, Reporter; D012240:Rhodococcus / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013302:Streptomyces / Q000737:chemistry / Q000378:metabolism; D015025:Zearalenone / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004785:Environmental Pollutants; D004967:Estrogens; D015025:Zearalenone