title: Shotgun proteomic analysis of the Mexican lime tree infected with "CandidatusPhytoplasma aurantifolia".
abstract: Infection of Mexican lime trees (Citrus aurantifolia L.) with the specialized bacterium "CandidatusPhytoplasma aurantifolia" causes witches' broom disease. Witches' broom disease has the potential to cause significant economic losses throughout western Asia and North Africa. We used label-free quantitative shotgun proteomics to study changes in the proteome of Mexican lime trees in response to infection by "Ca. Phytoplasma aurantifolia". Of 990 proteins present in five replicates of healthy and infected plants, the abundances of 448 proteins changed significantly in response to phytoplasma infection. Of these, 274 proteins were less abundant in infected plants than in healthy plants, and 174 proteins were more abundant in infected plants than in healthy plants. These 448 proteins were involved in stress response, metabolism, growth and development, signal transduction, photosynthesis, cell cycle, and cell wall organization. Our results suggest that proteomic changes in response to infection by phytoplasmas might support phytoplasma nutrition by promoting alterations in the host's sugar metabolism, cell wall biosynthesis, and expression of defense-related proteins. Regulation of defense-related pathways suggests that defense compounds are induced in interactions with susceptible as well as resistant hosts, with the main differences between the two interactions being the speed and intensity of the response.
authors.affiliation: Department of Genomics, Agricultural Biotechnology Research Institute of Iran, Karaj, Tehran, Iran.
authors.forename: Aboozar
authors.initials: A
authors.lastname: Monavarfeshani
authors.forename: Mehdi
authors.initials: M
authors.lastname: Mirzaei
authors.forename: Elham
authors.initials: E
authors.lastname: Sarhadi
authors.forename: Ardeshir
authors.initials: A
authors.lastname: Amirkhani
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Khayam Nekouei
authors.forename: Paul A
authors.initials: PA
authors.lastname: Haynes
authors.forename: Mohsen
authors.initials: M
authors.lastname: Mardi
authors.forename: Ghasem Hosseini
authors.initials: GH
authors.lastname: Salekdeh
mesh_terms: D002453:Cell Cycle / Q000235:genetics; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D032082:Citrus aurantiifolia / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D018506:Gene Expression Regulation, Plant; D054884:Host-Pathogen Interactions; D010788:Photosynthesis / Q000235:genetics; D045582:Phytoplasma / Q000378:metabolism / Q000472:pathogenicity; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D010940:Plant Proteins; D020543:Proteome