title: In vitro cytotoxicity and genotoxicity studies of titanium dioxide (TiO2) nanoparticles in Chinese hamster lung fibroblast cells.
abstract: There are increasing safety concerns about the development and abundant use of nanoparticles. The unique physical and chemical characteristics of titanium dioxide (TiO2) nanoparticles result in different chemical and biological activities compared to their larger micron-sized counterparts, and can subsequently play an important role in influencing toxicity. Therefore, our objective was to investigate the cytotoxicity and genotoxicity of commercially available TiO2 nanoparticles with respect to their selected physicochemical properties, as well as the role of surface coating of these nanoparticles. While all types of tested TiO2 samples decrease cell viability in a mass-based concentration- and size-dependent manner, the polyacrylate-coated nano-TiO2 product was only cytotoxic at higher concentrations. A similar pattern of response was observed for induction of apoptosis/necrosis, and no DNA damage was detected in the polyacrylate-coated nano-TiO2 model. Given the increasing production of TiO2 nanoparticles, toxicological studies should take into account the physiochemical properties of these nanoparticles that may help researchers to develop new nanoparticles with minimum toxicity.
authors.affiliation: National Research Council Canada, 6100 Royalmount Ave., Montréal, QC H4P 2R2, Canada. mahsa.hamzeh@cnrc-nrc.gc.ca
authors.forename: Mahsa
authors.initials: M
authors.lastname: Hamzeh
authors.forename: Geoffrey I
authors.initials: GI
authors.lastname: Sunahara
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival; D020552:Comet Assay; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D008168:Lung / Q000166:cytology; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D009336:Necrosis / Q000139:chemically induced; D017382:Reactive Oxygen Species / Q000378:metabolism; D014025:Titanium / Q000737:chemistry* / Q000633:toxicity*
chemical_list: D017382:Reactive Oxygen Species; C009495:titanium dioxide; D014025:Titanium