title: Mechanisms of linezolid resistance among Staphylococci in a tertiary hospital.
abstract: The mechanisms of linezolid resistance among 86 staphylococcal isolates from two intensive care units were investigated. The most frequent was the G2576T mutation in the 23S rRNA (82%). The cfr gene was found in 17% of the isolates, seven S. aureus and eight S. epidermidis isolates. Four of the S. epidermidis isolates had the G2576T mutation and the cfr gene. In four S. haemolyticus isolates, the mechanism could not be identified.
authors.affiliation: Servicio de Microbiología, Hospital Universitario La Paz, IdiPAZ, Madrid, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Quiles-Melero
authors.forename: Rosa
authors.initials: R
authors.lastname: Gómez-Gil
authors.forename: María Pilar
authors.initials: MP
authors.lastname: Romero-Gómez
authors.forename: Ana María
authors.initials: AM
authors.lastname: Sánchez-Díaz
authors.forename: Manuela
authors.initials: M
authors.lastname: de Pablos
authors.forename: Julio
authors.initials: J
authors.lastname: García-Rodriguez
authors.forename: Avelino
authors.initials: A
authors.lastname: Gutiérrez
authors.forename: Jesús
authors.initials: J
authors.lastname: Mingorance
mesh_terms: D000081:Acetamides / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D024881:Drug Resistance, Bacterial; D006801:Humans; D007362:Intensive Care Units; D000069349:Linezolid; D023303:Oxazolidinones / Q000494:pharmacology*; D017354:Point Mutation; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D041162:Staphylococcus haemolyticus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D062606:Tertiary Care Centers
chemical_list: D000081:Acetamides; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C539943:CFR protein, Staphylococcus aureus; D023303:Oxazolidinones; D012338:RNA, Ribosomal, 23S; D000069349:Linezolid