title: Comparing cell viability and ethanol fermentation of the thermotolerant yeast Kluyveromyces marxianus and Saccharomyces cerevisiae on steam-exploded biomass treated with laccase.
abstract: In this study, the thermotolerant yeast Kluyveromyces marxianus CECT 10875 was compared to the industrial strain Saccharomyces cerevisiae Ethanol Red for lignocellulosic ethanol production. For it, whole slurry from steam-exploded wheat straw was used as raw material, and two process configurations, simultaneous saccharification and fermentation (SSF) and presaccharification and simultaneous saccharification and fermentation (PSSF), were evaluated. Compared to S. cerevisiae, which was able to produce ethanol in both process configurations, K. marxianus was inhibited, and neither growth nor ethanol production occurred during the processes. However, laccase treatment of the whole slurry removed specifically lignin phenols from the overall inhibitory compounds present in the slurry and triggered the fermentation by K. marxianus, attaining final ethanol concentrations and yields comparable to those obtained by S. cerevisiae.
authors.affiliation: IMDEA Energía, Biotechnological Processes for Energy Production Unit, Móstoles, Madrid 28935, Spain.
authors.forename: Antonio D
authors.initials: AD
authors.lastname: Moreno
authors.forename: David
authors.initials: D
authors.lastname: Ibarra
authors.forename: Ignacio
authors.initials: I
authors.lastname: Ballesteros
authors.forename: Alberto
authors.initials: A
authors.lastname: González
authors.forename: Mercedes
authors.initials: M
authors.lastname: Ballesteros
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects; D018533:Biomass; D050260:Carbohydrate Metabolism / Q000187:drug effects; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects*; D007716:Kluyveromyces / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D042845:Laccase / Q000494:pharmacology*; D050296:Microbial Viability / Q000187:drug effects*; D055494:Pycnoporus / Q000201:enzymology; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013227:Steam; D013696:Temperature; D013997:Time Factors; D014908:Triticum / Q000737:chemistry
chemical_list: D013227:Steam; D000431:Ethanol; D042845:Laccase