title: Hydrogen inhalation decreases lung graft injury in brain-dead donor rats.
abstract: BACKGROUND
  The process of brain death induces acute lung injury in donors and aggravates ischemia-reperfusion injury (IRI) in grafts. Hydrogen, a new anti-oxidant, attenuates IRI in several organ transplant models. We examined whether 2% inhaled hydrogen would show favorable effects on lung grafts from brain-dead donor rats.
  
  
  METHODS
  Brain-dead donor rats inhaled mixed gases with either 50% oxygen and 50% nitrogen or mixed gases with 2% hydrogen, 50% oxygen and 48% nitrogen for 2 hours. The recipients inhaled the same gas as the donors and were euthanized 2 hours after lung transplantation.
  
  
  RESULTS
  Hydrogen improved PaO(2)/FIO(2) and PVO(2)/FIO(2) from the arterial and pulmonary venous blood in recipients and decreased the lung injury score in grafts from brain-dead donors. Hydrogen decreased the amount of IL-8 and TNF-α in serum, inhibited the activity of malondialdehyde and myeloperoxidase, and increased the activity of superoxide dismutase in the lung grafts from brain-dead donors. Furthermore, hydrogen decreased the apoptotic index of the cells and inhibited the protein expression of intercellular adhesion molecule-1 and caspase-3 in lung grafts from brain-dead donors.
  
  
  CONCLUSIONS
  Hydrogen can exert protective effects on lung grafts from brain-dead donors through anti-inflammatory, anti-oxidant and anti-apoptotic mechanisms.
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Huacheng
authors.initials: H
authors.lastname: Zhou
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Fu
authors.forename: Yuting
authors.initials: Y
authors.lastname: Wei
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Liu
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Cui
authors.forename: Wanchao
authors.initials: W
authors.lastname: Yang
authors.forename: Wengang
authors.initials: W
authors.lastname: Ding
authors.forename: Peng
authors.initials: P
authors.lastname: Pan
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Li
mesh_terms: D055371:Acute Lung Injury / Q000517:prevention & control*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000502:physiology; D001926:Brain Death; D020170:Caspase 1 / Q000097:blood; D006859:Hydrogen / Q000494:pharmacology*; D020287:In Situ Nick-End Labeling; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D016209:Interleukin-8 / Q000097:blood; D016040:Lung Transplantation; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D014019:Tissue Donors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000975:Antioxidants; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D006859:Hydrogen; D020170:Caspase 1