title: Tangentially migrating neurons assemble a primary cilium that promotes their reorientation to the cortical plate.
abstract: In migrating neurons, the centrosome nucleates and anchors a polarized network of microtubules that directs organelle movements. We report here that the mother centriole of neurons migrating tangentially from the medial ganglionic eminence (MGE) assembles a short primary cilium and exposes this cilium to the cell surface by docking to the plasma membrane in the leading process. Primary cilia are built by intraflagellar transport (IFT), which is also required for Sonic hedgehog (Shh) signal transduction in vertebrates. We show that Shh pathway perturbations influenced the leading process morphology and dynamics of MGE cells. Whereas Shh favored the exit of MGE cells away from their tangential migratory paths in the developing cortex, cyclopamine or invalidation of IFT genes maintained MGE cells in the tangential paths. Our findings show that signals transmitted through the primary cilium promote the escape of future GABAergic interneurons from their tangential routes to colonize the cortical plate.
authors.affiliation: INSERM, UMRS-839, Institut du Fer à Moulin, 17 rue du Fer à Moulin, 75005 Paris, France.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Baudoin
authors.forename: Lucie
authors.initials: L
authors.lastname: Viou
authors.forename: Pierre-Serge
authors.initials: PS
authors.lastname: Launay
authors.forename: Camilla
authors.initials: C
authors.lastname: Luccardini
authors.forename: Sergio
authors.initials: S
authors.lastname: Espeso Gil
authors.forename: Vera
authors.initials: V
authors.lastname: Kiyasova
authors.forename: Théano
authors.initials: T
authors.lastname: Irinopoulou
authors.forename: Chantal
authors.initials: C
authors.lastname: Alvarez
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Rio
authors.forename: Thomas
authors.initials: T
authors.lastname: Boudier
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Lechaire
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Kessaris
authors.forename: Nathalie
authors.initials: N
authors.lastname: Spassky
authors.forename: Christine
authors.initials: C
authors.lastname: Métin
mesh_terms: D000818:Animals; D001479:Basal Ganglia / Q000166:cytology / Q000196:embryology; D002465:Cell Movement / Q000502:physiology*; D016764:Cell Polarity / Q000502:physiology; D002502:Centrioles / Q000502:physiology; D018385:Centrosome / Q000502:physiology*; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology*; D002923:Cilia / Q000502:physiology*; D053823:Hedgehog Proteins / Q000502:physiology*; D051379:Mice; D008818:Mice, Neurologic Mutants; D008822:Mice, Transgenic; D055495:Neurogenesis / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology
chemical_list: D053823:Hedgehog Proteins; C465570:Shh protein, mouse