title: Antiproliferative withanolides from Datura wrightii.
abstract: A new withanolide, named withawrightolide (1), and four known withanolides (2-5) were isolated from the aerial parts of Datura wrightii. The structure of compound 1 was elucidated through 2D NMR and other spectroscopic techniques. In addition, the structure of withametelin L (2) was confirmed by X-ray crystallographic analysis. Using MTS viability assays, withanolides 1-5 showed antiproliferative activities against human glioblastoma (U251 and U87), head and neck squamous cell carcinoma (MDA-1986), and normal fetal lung fibroblast (MRC-5) cells with IC50 values in the range between 0.56 and 5.6 μM.
authors.affiliation: Department of Medicinal Chemistry, School of Pharmacy, University of Kansas, Lawrence, Kansas 66045, USA.
authors.forename: Huaping
authors.initials: H
authors.lastname: Zhang
authors.forename: Joseph
authors.initials: J
authors.lastname: Bazzill
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Gallagher
authors.forename: Chitra
authors.initials: C
authors.lastname: Subramanian
authors.forename: Patrick T
authors.initials: PT
authors.lastname: Grogan
authors.forename: Victor W
authors.initials: VW
authors.lastname: Day
authors.forename: Kelly
authors.initials: K
authors.lastname: Kindscher
authors.forename: Mark S
authors.initials: MS
authors.lastname: Cohen
authors.forename: Barbara N
authors.initials: BN
authors.lastname: Timmermann
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy; D018360:Crystallography, X-Ray; D032302:Datura / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D005347:Fibroblasts / Q000187:drug effects; D005909:Glioblastoma / Q000188:drug therapy; D006801:Humans; D020128:Inhibitory Concentration 50; D007615:Kansas; D008168:Lung / Q000166:cytology / Q000187:drug effects; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D054358:Withanolides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D054358:Withanolides