title: Polychlorinated biphenyl (118) activates osteoclasts and induces bone resorption in goldfish.
abstract: To analyze the effect of polychlorinated biphenyl (PCB) 118 on fish bone metabolism, we examined osteoclastic and osteoblastic activities, as well as plasma calcium levels, in the scales of PCB (118)-injected goldfish. In addition, effect of PCB (118) on osteoclasts and osteoblasts was investigated in vitro. Immature goldfish, in which the endogenous effects of sex steroids are negligible, were used. PCB (118) was solubilized in dimethyl sulfoxide at a concentration of 10 ppm. At 1 and 2 days after PCB (118) injection (100 ng/g body weight), both osteoclastic and osteoblastic activities, and plasma calcium levels were measured. In an in vitro study, then, both osteoclastic and osteoblastic activities as well as each marker mRNA expression were examined. At 2 days, scale osteoclastic activity in PCB (118)-injected goldfish increased significantly, while osteoblastic activity did not change significantly. Corresponding to osteoclastic activity, plasma calcium levels increased significantly at 2 days after PCB (118) administration. Osteoclastic activation also occurred in the marker enzyme activities and mRNA expressions in vitro. Thus, we conclude that PCB (118) disrupts bone metabolism in goldfish both in vivo and in vitro experiments.
authors.affiliation: Noto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Ogi, Noto-cho, Housu-gun, Ishikawa, 927-0553, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Yachiguchi
authors.forename: Noriko
authors.initials: N
authors.lastname: Matsumoto
authors.forename: Yuki
authors.initials: Y
authors.lastname: Haga
authors.forename: Motoharu
authors.initials: M
authors.lastname: Suzuki
authors.forename: Chisato
authors.initials: C
authors.lastname: Matsumura
authors.forename: Masahiro
authors.initials: M
authors.lastname: Tsurukawa
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Okuno
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nakano
authors.forename: Kimi
authors.initials: K
authors.lastname: Kawabe
authors.forename: Kei-ichiro
authors.initials: K
authors.lastname: Kitamura
authors.forename: Akira
authors.initials: A
authors.lastname: Toriba
authors.forename: Kazuichi
authors.initials: K
authors.lastname: Hayakawa
authors.forename: Vishwajit S
authors.initials: VS
authors.lastname: Chowdhury
authors.forename: Masato
authors.initials: M
authors.lastname: Endo
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Chiba
authors.forename: Toshio
authors.initials: T
authors.lastname: Sekiguchi
authors.forename: Masaki
authors.initials: M
authors.lastname: Nakano
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Tabuchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
authors.forename: Shigehito
authors.initials: S
authors.lastname: Wada
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mishima
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Hattori
authors.forename: Nobuo
authors.initials: N
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D001862:Bone Resorption; D006054:Goldfish / Q000378:metabolism / Q000502:physiology; D010006:Osteoblasts; D010010:Osteoclasts / Q000187:drug effects / Q000502:physiology*; D011078:Polychlorinated Biphenyls / Q000378:metabolism / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls