title: Use of organic waste from the brewery industry for high-density cultivation of the docosahexaenoic acid-rich microalga, Aurantiochytrium sp. KRS101.
abstract: In the present study, spent yeast from a brewery was used as the growth substrate for the docosahexaenoic acid (DHA)-rich microalga, Aurantiochytrium sp. KRS101. A significant biomass yield (6.69 g/l/d) was obtained using only spent yeast as the growth substrate, with simple stirring as pretreatment. Maximization of nutrient utilization through the use of stepwise cultivation increased the yield to 31.8 g/l of biomass. DHA constituted 38.2% (w/w) of the total fatty acids, and the highest DHA productivity was observed when the C/N ratio was 20:1 (w/w). Spent yeast thus served as a good growth substrate for the production of DHA. Economic assessment revealed that stepwise cultivation using spent yeast as either the sole growth substrate or as a nutrient source could substantially reduce the production cost of microalgal DHA.
authors.affiliation: Environmental and Energy Program, Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehakno, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Byung-Gon
authors.initials: BG
authors.lastname: Ryu
authors.forename: Kyochan
authors.initials: K
authors.lastname: Kim
authors.forename: Jungmin
authors.initials: J
authors.lastname: Kim
authors.forename: Jong-In
authors.initials: JI
authors.lastname: Han
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Yang
mesh_terms: D061249:Batch Cell Culture Techniques / Q000191:economics* / Q000379:methods; D019149:Bioreactors / Q000191:economics* / Q000382:microbiology*; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D007220:Industrial Waste / Q000517:prevention & control*; D058086:Microalgae / Q000378:metabolism*; D009930:Organic Chemicals / Q000378:metabolism*; D056910:Republic of Korea
chemical_list: D007220:Industrial Waste; D009930:Organic Chemicals; D004281:Docosahexaenoic Acids