title: Computational interrogation of cis-regulatory elements of genes that are common targets of luteotropin and luteolysin in the primate corpus luteum.
abstract: The rapid recent increase in microarray-based gene expression studies in the corpus luteum (CL) utilizing macaque models gathered increasing volume of data in publically accessible microarray expression databases. Examining gene pathways in different functional states of CL may help to understand the factors that control luteal function and hence human fertility. Co-regulation of genes in microarray experiments may imply common transcriptional regulation by sequence-specific DNA-binding transcriptional factors. We have computationally analyzed the transcription factor binding sites (TFBS) in a previously reported macaque luteal microarray gene set (n=15) that are common targets of luteotropin (luteinizing hormone (LH) and human chorionic gonadotropin (hCG)) and luteolysin (prostaglandin (PG) F(2)α). This in silico approach can reveal transcriptional networks that control these important genes which are representative of the interplay between luteotropic and luteolytic factors in the control of luteal function. Our computational analyses revealed 6 matrix families whose binding sites are significantly over-represented in promoters of these genes. The roles of these factors are discussed, which might help to understand the transcriptional regulatory network in the control of luteal function. These factors might be promising experimental targets for investigation of human luteal insufficiency.
authors.affiliation: Centre for Biomedical Research, Vellore Institute of Technology University, Vellore, India. surepadman@rediffmail.com
authors.forename: Padmanaban S
authors.initials: PS
authors.lastname: Suresh
authors.forename: Thejaswini
authors.initials: T
authors.lastname: Venkatesh
mesh_terms: D000818:Animals; D001665:Binding Sites; D006063:Chorionic Gonadotropin / Q000502:physiology*; D003338:Corpus Luteum / Q000378:metabolism*; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D015237:Dinoprost / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation; D005796:Genes; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D007986:Luteinizing Hormone / Q000502:physiology*; D008255:Macaca radiata / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D050783:Proto-Oncogene Proteins c-ets / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D017422:Sequence Analysis, DNA; D016329:Sp1 Transcription Factor / Q000235:genetics / Q000378:metabolism / Q000502:physiology
chemical_list: D006063:Chorionic Gonadotropin; D017362:Cyclic AMP Response Element-Binding Protein; D051741:Kruppel-Like Transcription Factors; D050783:Proto-Oncogene Proteins c-ets; D016329:Sp1 Transcription Factor; D007986:Luteinizing Hormone; D015237:Dinoprost