title: HAVcR-1 expression in human colorectal cancer and its effects on colorectal cancer cells in vitro.
abstract: BACKGROUND
  Hepatitis A virus cellular receptor 1 (HAVcR-1) may function as part of the regulatory apparatus for tight junction in human endothelial cells, and is overexpressed in renal cell and ovarian clear cell carcinomas.
  
  
  METHODS
  HAVcR-1 expression in colorectal cancer and matched background tissues was determined using quantitative-PCR. HAVcR-1 was overexpressed in three colorectal cancer cell lines and the effect of this overexpression was assessed by changes in cell behaviour.
  
  
  RESULTS
  HAVcR-1 expression was increased in colorectal cancer (p<0.001) and the disease-free time of patients with high levels of HAVcR-1 expression was significantly longer (p=0.045). HAVcR-1 overexpression reduced colorectal cancer cell adhesion and invasion (p<0.016).
  
  
  CONCLUSIONS
  HAVcR-1 overexpression may be a diagnostic biomarker for colorectal cancer and a prognostic marker for a longer disease-free interval after surgery for colorectal cancer. HAVcR-1 overexpression can reduce cell adhesion and invasion and could thus be involved in the metastatic cascade.
authors.affiliation: Metastasis and Angiogenesis Research Group, Institute of Cancer and Genetics, Cardiff University School of Medicine, Heath Park, Cardiff CF14 4XN, UK.
authors.forename: Youxin
authors.initials: Y
authors.lastname: Wang
authors.forename: Tracey A
authors.initials: TA
authors.lastname: Martin
authors.forename: Wen G
authors.initials: WG
authors.lastname: Jiang
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D018938:Caco-2 Cells; D002448:Cell Adhesion / Q000235:genetics; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D015972:Gene Expression Regulation, Neoplastic; D000072596:Hepatitis A Virus Cellular Receptor 1; D006801:Humans; D053208:Kaplan-Meier Estimate; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000235:genetics; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011379:Prognosis; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; C491143:HAVCR1 protein, human; D000072596:Hepatitis A Virus Cellular Receptor 1; D008562:Membrane Glycoproteins; D011991:Receptors, Virus