title: [Researches on the relative expression of HQT gene in different organs of Lonicera japonica].
abstract: OBJECTIVE
  To reveal the correlation between HQT gene and the biosynthesis of chlorogenic acid in Lonicera japonica.
  
  
  METHODS
  RT-PCR was used to measure the relative expression of HQT gene and reference gene Actin, and agarose gel electrophoresis was used to analyse the PCR results.
  
  
  RESULTS
  The brightness of Actin gene strips of different organs was properly similar to each other,but the brightness of HQT gene strips was significantly different. HQT gene strips of alabastrum were the brightest,the brightness of HQT gene strip of leaves took the second place, and the brightness of HQT gene strips of stems was very faint. This result was in accordance with the content of chlorogenic acid in different organs of Lonicera japonica.
  
  
  CONCLUSIONS
  The expression of HQT gene with the biosynthesis of chlorogenic acid has necessary relation with Lonicera japonica.
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. liuyliwd@vip.sina.com
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiao-Xiao
authors.initials: XX
authors.lastname: Peng
authors.forename: Sha-Sha
authors.initials: SS
authors.lastname: Zhu
authors.forename: Gen-Ben
authors.initials: GB
authors.lastname: Bai
mesh_terms: D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D002726:Chlorogenic Acid / Q000378:metabolism*; D017931:DNA Primers; D018076:DNA, Complementary / Q000032:analysis; D035264:Flowers / Q000737:chemistry / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D031239:Lonicera / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D018547:Plant Stems / Q000737:chemistry / Q000378:metabolism; D010946:Plants, Medicinal / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D002726:Chlorogenic Acid; D000217:Acyltransferases; C021394:hydroxycinnamoyl-CoA-quinate transferase