title: Inhibitory effect on TNF-α-induced IL-8 secretion in HT-29 cell line by glyceroglycolipids from the leaves of Ficus microcarpa.
abstract: Bioassay-guided fractionation based on the anti-inflammatory activity of a methanol extract of Ficus microcarpa leaves led to the isolation of seven galactolipids: 2(S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl-O-β-D-galactopyranoside (1), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-O-β-D-galactopyranoside (2), (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-O-β-D-galactopyranoside (3), (2S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl-6'-O-(α-D-galactopyranosyl)-β-D-galactopyranoside (4), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-6'-O-(α-D-galactopyranosyl)-β-D-galactopyranoside (5), gingerglycolipid B (6), and (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-6'-O-(α-D-galactopyranosyl)-β-D-galactopyranoside (7). Their chemical structures were elucidated by mass, 1D-, and 2D-NMR spectroscopic methods as well as chemical methods. The antiinflammatory effect of these compounds on TNF-α induced IL-8 secretion in the HT-29 cell line was evaluated. All above galactolipids showed significant inhibition ranging 40% at a concentration of 50 μM. The results suggest that galactolipids from the leaves of F. microcarpa may be used as potent anti-inflammatory agents.
authors.affiliation: Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam.
authors.forename: Phan Van
authors.initials: PV
authors.lastname: Kiem
authors.forename: Chau Van
authors.initials: CV
authors.lastname: Minh
authors.forename: Nguyen Xuan
authors.initials: NX
authors.lastname: Nhiem
authors.forename: Nguyen Xuan
authors.initials: NX
authors.lastname: Cuong
authors.forename: Bui Huu
authors.initials: BH
authors.lastname: Tai
authors.forename: Tran Hong
authors.initials: TH
authors.lastname: Quang
authors.forename: Hoang Le Tuan
authors.initials: Hle T
authors.lastname: Anh
authors.forename: Pham Hai
authors.initials: PH
authors.lastname: Yen
authors.forename: Ninh Khac
authors.initials: NK
authors.lastname: Ban
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Mingjie
authors.initials: M
authors.lastname: Xin
authors.forename: Ji-Yun
authors.initials: JY
authors.lastname: Cha
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D030681:Ficus; D006017:Glycolipids / Q000302:isolation & purification / Q000502:physiology*; D019073:HT29 Cells; D006801:Humans; D016209:Interleukin-8 / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000502:physiology*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D006017:Glycolipids; D016209:Interleukin-8; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; C015903:glycerolglycolipids