title: Ischemic postconditioning fails to protect against neonatal cerebral stroke.
abstract: The lack of efficient neuroprotective strategies for neonatal stroke could be ascribed to pathogenic ischemic processes differentiating adults and neonates. We explored this hypothesis using a rat model of neonatal ischemia induced by permanent occlusion of the left distal middle cerebral artery combined with 50 min of occlusion of both common carotid arteries (CCA). Postconditioning was performed by repetitive brief release and occlusion (30 s, 1 and/or 5 min) of CCA after 50 min of CCA occlusion. Alternative reperfusion was generated by controlled release of the bilateral CCA occlusion. Blood-flow velocities in the left internal carotid artery were measured using color-coded pulsed Doppler ultrasound imaging. Cortical perfusion was measured using laser Doppler. Cerebrovascular vasoreactivity was evaluated after inhalation with the hypercapnic gas or inhaled nitric oxide (NO). Whatever the type of serial mechanical interruptions of blood flow at reperfusion, postconditioning did not reduce infarct volume after 72 hours. A gradual perfusion was found during early re-flow both in the left internal carotid artery and in the cortical penumbra. The absence of acute hyperemia during early CCA re-flow, and the lack of NO-dependent vasoreactivity in P7 rat brain could in part explain the inefficiency of ischemic postconditioning after ischemia-reperfusion.
authors.affiliation: Univ Paris Diderot, Sorbonne Paris Cité, INSERM, U676, Paris, France.
authors.forename: Pierre-Louis
authors.initials: PL
authors.lastname: Leger
authors.forename: Philippe
authors.initials: P
authors.lastname: Bonnin
authors.forename: Thao
authors.initials: T
authors.lastname: Nguyen
authors.forename: Sylvain
authors.initials: S
authors.lastname: Renolleau
authors.forename: Olivier
authors.initials: O
authors.lastname: Baud
authors.forename: Christiane
authors.initials: C
authors.lastname: Charriaut-Marlangue
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001783:Blood Flow Velocity / Q000502:physiology; D001921:Brain / Q000473:pathology* / Q000503:physiopathology; D002545:Brain Ischemia / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D002343:Carotid Artery, Internal / Q000473:pathology / Q000503:physiopathology; D002560:Cerebrovascular Circulation / Q000502:physiology*; D057775:Ischemic Postconditioning; D051381:Rats; D017208:Rats, Wistar; D020521:Stroke / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*