title: An in vitro-in silico-in vivo approach to predicting the oral pharmacokinetic profile of salts of weak acids: case example dantrolene.
abstract: The aim of this research was to characterize both the dissolution and precipitation kinetics of a model poorly water soluble drug, dantrolene, from its dissolution data, and to develop a predictive model for its oral pharmacokinetics. Physiologically based pharmacokinetic (PBPK) models were coupled with biorelevant dissolution and precipitation data to predict PK profiles of dantrolene in fasted humans. The paddle method was used to obtain the dissolution profiles of free acid form of dantrolene, dantrolene sodium, and the corresponding commercial product in biorelevant media. The Noyes-Whitney model was used to describe the dissolution kinetics, and a first-order equation was employed for estimating the precipitation kinetics from these dissolution profiles. The PBPK model was set up using STELLA software. After an initial phase of dissolution, the concentration of dantrolene in FaSSGF and FaSSIF-V2 started to decrease again. From the overall dissolution profiles, the dissolution rate constant based on the Noyes-Whitney theory for dissolution and the first-order precipitation rate constant were calculated. The PK profiles predicted under consideration of the precipitation were close to the observed PK profile. By contrast, when only the dissolution was considered, the absorption rate was overestimated. These results indicate that PBPK modeling with dissolution and precipitation kinetics is useful for the predicting the PK profile after oral administration of dantrolene from the sodium salt and suggests a way forward for predicting PK profiles of other salts of weak acids that are poorly soluble.
authors.affiliation: Institute of Pharmaceutical Technology, Goethe University, Frankfurt am Main, Germany.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kambayashi
authors.forename: Jennifer B
authors.initials: JB
authors.lastname: Dressman
mesh_terms: D000284:Administration, Oral; D003198:Computer Simulation; D003620:Dantrolene / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D005544:Forecasting; D005746:Gastric Emptying / Q000187:drug effects / Q000502:physiology; D006801:Humans; D008297:Male; D008954:Models, Biological; D012492:Salts; D012995:Solubility / Q000187:drug effects
chemical_list: D012492:Salts; D003620:Dantrolene