title: The MTT assay is a rapid and reliable quantitative method to assess Staphylococcus aureus induced endothelial cell damage.
abstract: We established the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to assess damage induced by Staphylococcus aureus in human umbilical vein endothelial cells (HUVECs). The MTT assay was comparable to the previously published (51)chromium release assay. MTT reduction by intracellular S. aureus was negligible and therefore permits assessment of S. aureus induced EC damage.
authors.affiliation: Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Zurich, Switzerland. katiseidl@gmail.com
authors.forename: Kati
authors.initials: K
authors.lastname: Seidl
authors.forename: Annelies S
authors.initials: AS
authors.lastname: Zinkernagel
mesh_terms: D002470:Cell Survival; D002478:Cells, Cultured; D002860:Chromium Radioisotopes / Q000378:metabolism; D003584:Cytological Techniques / Q000379:methods*; D042783:Endothelial Cells / Q000382:microbiology* / Q000502:physiology*; D006801:Humans; D013194:Staining and Labeling / Q000379:methods*; D013211:Staphylococcus aureus / Q000472:pathogenicity*; D013778:Tetrazolium Salts / Q000378:metabolism*; D013844:Thiazoles / Q000378:metabolism*
chemical_list: D002860:Chromium Radioisotopes; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue