title: A Weibull model to describe antimicrobial kinetics of oregano and lemongrass essential oils against Salmonella Enteritidis in ground beef during refrigerated storage.
abstract: The antimicrobial effect of oregano (Origanum vulgare L.) and lemongrass (Cymbopogon citratus (DC.) Stapf.) essential oils (EOs) against Salmonella enterica serotype Enteritidis in in vitro experiments, and inoculated in ground bovine meat during refrigerated storage (4±2 °C) for 6 days was evaluated. The Weibull model was tested to fit survival/inactivation bacterial curves (estimating of p and δ parameters). The minimum inhibitory concentration (MIC) value for both EOs on S. Enteritidis was 3.90 μl/ml. The EO concentrations applied in the ground beef were 3.90, 7.80 and 15.60 μl/g, based on MIC levels and possible activity reduction by food constituents. Both evaluated EOs in all tested levels, showed antimicrobial effects, with microbial populations reducing (p≤0.05) along time storage. Evaluating fit-quality parameters (RSS and RSE) Weibull models are able to describe the inactivation curves of EOs against S. Enteritidis. The application of EOs in processed meats can be used to control pathogens during refrigerated shelf-life.
authors.affiliation: State University of Campinas - UNICAMP, Department of Food Technology, Food Engineering Faculty, FEA/UNICAMP, Campinas, SP, CEP 13083-862, Brazil. thales@fea.unicamp.br
authors.forename: Thales Leandro Coutinho
authors.initials: TL
authors.lastname: de Oliveira
authors.forename: Rodrigo de Araújo
authors.initials: Rde A
authors.lastname: Soares
authors.forename: Roberta Hilsdorf
authors.initials: RH
authors.lastname: Piccoli
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002417:Cattle; D031743:Cymbopogon / Q000737:chemistry*; D005516:Food Microbiology; D061353:Food Storage; D008460:Meat / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D008954:Models, Biological; D009822:Oils, Volatile / Q000494:pharmacology*; D031341:Origanum / Q000737:chemistry*; D012034:Refrigeration; D012477:Salmonella enteritidis / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D009822:Oils, Volatile