title: Enhanced transgene expression from chromatinized plasmid DNA in mouse liver.
abstract: Plasmid DNA was chromatinized with core histones (H2A, H2B, H3, and H4) in vitro and was delivered into mouse liver by hydrodynamics-based administration. Transgene expression from the chromatinized plasmid DNA was more efficient than that from plasmid DNA delivered in the naked form. The use of acetylation-enriched histones isolated from cells treated with a histone deacetylase inhibitor (trichostatin A) seemed to be more effective. These results indicated that chromatinized plasmid DNA is useful for efficient transgene expression in vivo.
authors.affiliation: Graduate School of Science and Engineering, Ehime University, Matsuyama 790-8577, Japan. hirokam@ehime-u.ac.jp
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kamiya
authors.forename: Shiho
authors.initials: S
authors.lastname: Miyamoto
authors.forename: Hitomi
authors.initials: H
authors.lastname: Goto
authors.forename: Genki N
authors.initials: GN
authors.lastname: Kanda
authors.forename: Miwako
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Ichiro
authors.initials: I
authors.lastname: Matsuoka
authors.forename: Hideyoshi
authors.initials: H
authors.lastname: Harashima
mesh_terms: D000818:Animals; D002843:Chromatin / Q000737:chemistry; D004247:DNA / Q000008:administration & dosage*; D005260:Female; D005786:Gene Expression Regulation; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006657:Histones / Q000378:metabolism*; D006877:Hydroxamic Acids / Q000494:pharmacology; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D019076:Transgenes
chemical_list: D002843:Chromatin; D056572:Histone Deacetylase Inhibitors; D006657:Histones; D006877:Hydroxamic Acids; C012589:trichostatin A; D004247:DNA