title: Polyvinyl alcohol and polyethylene glycol form polymer bodies in the periplasm of Sphingomonads that are able to assimilate them.
abstract: Scanning electron microscopy (SEM) shows remarkable morphological surface changes in Sphingopyxis sp. 113P3 cells grown in polyvinyl alcohol (PVA) but not in Luria-Bertani medium (LB) (Hu et al. in Arch Microbiol 188: 235-241, 2007). However, transmission electron microscopy showed no surface changes in PVA-grown cells and revealed the presence of polymer bodies in the periplasm of PVA-grown cells, which were not observed in LB-grown cells. The presence of polymer bodies was supported by low-vacuum SEM observation of PVA- and LB-grown cells of strain 113P3, and the presence of similar polymer bodies was also found when Sphingopyxis macrogoltabida 103 and S. terrae were grown in polyethylene glycol (PEG). The extraction of PVA and PEG from the periplasmic fraction of cells using a modified Anraku and Heppel method and their analysis by MALDI-TOF mass spectrometry strongly suggested that the polymer bodies are composed of PVA and PEG, respectively, in Sphingopyxis sp. 113P3 (PVA degrader) and Sphingopyxis macrogoltabida 103 or S. terrae (PEG degraders). PEG-grown S. macrogoltabida 103 and S. terrae showed higher transport of (14)C-PEG 4000 than LB-grown cells. Recombinant PegB (TonB-dependent receptor-like protein consisting of a barrel structure) interacted with PEG 200, 4000 and 20000, suggesting that the barrel protein in the outer membrane contributes to the transport of PEG into the periplasm.
authors.affiliation: Center for Nanomaterials and Devices, Kyoto Institute of Technology, Matsugasaki, Kyoto 606-8585, Japan. fkawai@kit.ac.jp
authors.forename: Fusako
authors.initials: F
authors.lastname: Kawai
authors.forename: Sakihito
authors.initials: S
authors.lastname: Kitajima
authors.forename: Kenji
authors.initials: K
authors.lastname: Oda
authors.forename: Takahiko
authors.initials: T
authors.lastname: Higasa
authors.forename: Jittima
authors.initials: J
authors.lastname: Charoenpanich
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Hu
authors.forename: Rie
authors.initials: R
authors.lastname: Mamoto
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D019897:Periplasm / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D042301:Sphingomonadaceae / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D011108:Polymers; C057616:tonB protein, Bacteria; D011092:Polyethylene Glycols; D011142:Polyvinyl Alcohol