title: Determination of azathioprine in bulk and pharmaceutical dosage form by HPTLC.
abstract: BACKGROUND
  An HPTLC method for analysis of Azathioprine in bulk and pharmaceutical formulation has been established and validated.
  
  
  METHODS
  The analyte was separated on aluminium plates precoated with silica gel 60 F254. The mobile phase was Ethyl acetate: Methanol: Triethylamine (4:1:0.5v/v). Quantification was done by densitometric scanning at 300nm.
  
  
  RESULTS
  Response was a linear function of Azathioprine concentration in the range of 200-1200 ng/band. The limit of detection and quantification for Azathioprine were 18.58 and 59.14 ng/band, respectively. Average recovery was 100.1% which shows that the method was free from interference from excipients present in the formulation.
  
  
  CONCLUSIONS
  The established method enabled accurate, precise, and rapid analysis of Azathioprine in bulk as well as pharmaceutical formulation.
authors.affiliation: Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dist. Dhule, Maharashtra, India.
authors.forename: Pritam S
authors.initials: PS
authors.lastname: Jain
authors.forename: Pradip
authors.initials: P
authors.lastname: Thakre
authors.forename: Amar J
authors.initials: AJ
authors.lastname: Chaudhari
authors.forename: Manish L
authors.initials: ML
authors.lastname: Chavhan
authors.forename: Sanjay J
authors.initials: SJ
authors.lastname: Surana