title: Polymorphism analysis and evaluation of 19 STR loci in the Han population of Southern China.
abstract: BACKGROUND
  The knowledge of allele and genotype frequencies is an essential prerequisite to the use of any human polymorphism in forensic medicine.
  
  
  OBJECTIVE
  To study the genetic polymorphism and evaluate the 19 STR loci using forensic medicine.
  
  
  METHODS
  Nineteen STR loci, which include D19S433, D5S818, D21S11, D18S51, D6S1043, D3S1358, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX, Penta E, TH01, D12S391, D2S1338 and FGA, were amplified simultaneously with Goldeneye(TM) DNA ID system 20A kit for 1161 unrelated Han individuals in Southern China. The PCR products were separated with the arrayed capillary electrophoresis. The allele frequency distribution and several parameters commonly used in forensic science were statistically analysed.
  
  
  RESULTS
  The observed heterozygosity (Hobs) of these 19 STR loci ranged from 0.6072-0.9070; the expected heterozygosity (Hexp) ranged from 0.6052-0.9117; the power of discrimination (PD) ranged from 0.7836-0.9662; the power of exclusion (PE) ranged from 0.3479-0.8227 for trio paternity cases and 0.1959-0.6988 for duo paternity cases; the polymorphic information content (PIC) ranged from 0.5443-0.9050.
  
  
  CONCLUSIONS
  The results indicate that 19 STR loci are polymorphic among the Han population in Southern China. This set of polymorphic STR loci is a useful tool in forensic paternity test and anthropological study.
authors.affiliation: Department of Forensic Biology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510089, PR China.
authors.forename: Dayue
authors.initials: D
authors.lastname: Tong
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: Xueling
authors.initials: X
authors.lastname: Ou
authors.forename: Weihong
authors.initials: W
authors.lastname: Chen
authors.forename: Sujuan
authors.initials: S
authors.lastname: Liu
authors.forename: Yinming
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hongyu
authors.initials: H
authors.lastname: Sun
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics*; D002681:China; D019075:Electrophoresis, Capillary; D005260:Female; D005787:Gene Frequency; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D055815:Young Adult