title: [Effects of cucurmosin combined with common chemotherapeutics on proliferation and apoptosis of NB4 cells].
abstract: This study was aimed to investigate the proliferation inhibition and apoptosis induction of cucurmosin (CUS) combined with all trans-retinoic acid (ATRA) or arsenic trioxide (ATO) on human acute promyelocytic leukemia cell line NB4. MTT method was used to determine the proliferative inhibition of CUS combined with ATRA or ATO on NB4 cells, and flow cytometry was used to determine the apoptosis induction effect of CUS combined with ATRA or ATO on NB4 cells. Jin's formula was used to assess the synergistic effect of this combinations. The results showed that, compared with single drug, the proliferation inhibitory ratio and apoptotic ratio of CUS combined with ATRA or ATO on NB4 cells was higher than CUS, ATRA and ATO alone. The synergistic index (q) were all larger than 0.85, and the combined effects were significant at low concentrations. It is concluded that the CUS combined with ATRA or ATO synergistically increases the effects of proliferative inhibition and apoptosis induction on NB4 cells.
authors.affiliation: School of Pharmacy, Fujian Medical University, Fuzhou 350004, Fujian province, China. jm_xie@hot mail.com
authors.forename: Jie-Ming
authors.initials: JM
authors.lastname: Xle
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.forename: Ting-Bo
authors.initials: TB
authors.lastname: Liu
authors.forename: Ming-Huang
authors.initials: MH
authors.lastname: Chen
authors.forename: Ai-Qin
authors.initials: AQ
authors.lastname: Yang
authors.forename: Pei
authors.initials: P
authors.lastname: Yang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004357:Drug Synergism; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology*; D010087:Oxides / Q000494:pharmacology; D010940:Plant Proteins / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D001152:Arsenicals; D010087:Oxides; D010940:Plant Proteins; C412450:cucurmosin protein, Cucurbita moschata; D014212:Tretinoin; D000077237:Arsenic Trioxide