title: The chronic effects of low lead level on the expressions of Nrf2 and Mrp1 of the testes in the rats.
abstract: Lead is linked to many reproductive problems. This study was to explore the chronic effects of low lead level on expressions of Nrf2 and Mrp1 in rats' testes. Maternal SD rats were administered lead acetate from 10 days before gestation to weaning at three doses respectively after randomization. From each group, 15 male offsprings were then chosen and administrated lead acetate from weaning to six months old at the doses of 0, 0.3 and 0.9g/L respectively. The dose administrations were through drinking water freely. The methods of RT-PCR, Western blotting and immunohistochemistry were used for Mrp1 and Nrf2 of the testes. Compared with control group, significant increases were observed in the expressions of Mrp1 and Nrf2 in two lead groups (P<0.05); nucleus translocation of Nrf2 was observed; both GST and GSH was decreased with increasing the lead dose. In conclusion, Mrp1 might play important roles in lead detoxification by Nrf2.
authors.affiliation: Department of Toxicology, School of Public Health, Wuhan University, DongHu Road 115, Wuhan 430071, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Junyi
authors.initials: J
authors.lastname: Fang
authors.forename: Shaoxin
authors.initials: S
authors.lastname: Huang
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Guangqin
authors.initials: G
authors.lastname: Fan
authors.forename: Chunhong
authors.initials: C
authors.lastname: Wang
mesh_terms: D000818:Animals; D004785:Environmental Pollutants / Q000097:blood / Q000493:pharmacokinetics / Q000633:toxicity*; D005260:Female; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007854:Lead / Q000097:blood / Q000493:pharmacokinetics / Q000633:toxicity*; D008297:Male; D008431:Maternal-Fetal Exchange; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000096:biosynthesis* / Q000235:genetics; D051267:NF-E2-Related Factor 2 / Q000096:biosynthesis* / Q000235:genetics; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013737:Testis / Q000187:drug effects* / Q000378:metabolism
chemical_list: D004785:Environmental Pollutants; D005978:Glutathione; D019803:Glutathione Disulfide; D005982:Glutathione Transferase; D007854:Lead; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D051267:NF-E2-Related Factor 2; D012333:RNA, Messenger; C495637:Nfe2l2 protein, rat; C421109:multidrug resistance-associated protein 1