title: Miiuy croaker (Miichthys miiuy) Peroxiredoxin2: molecular characterization, genomic structure and immune response against bacterial infection.
abstract: Peroxiredoxin2 (Prx2) protein is an important member in cellular antioxidant protein superfamily. Prx2 exists widely in prokaryotes and eukaryotes, it not only plays a part in eliminate reactive oxygen, but also takes effect in many other metabolic activities, such as stimulate epithelial cell proliferation, participate in the signal transduction in cells and so on. After molecular cloning we got that the complete cDNA sequence of Prx2 consists 882 bp, including a 5'-UTR of 46 bp, an open reading frame (ORF) of 591 bp, and a 3'-UTR of 245 bp. The complete gene of miiuy croaker Prx2 has 5 exons and 4 introns. The deduced 197 amino acid residues of miiuy croaker Prx2 consists a Val-Cys-Pro (VCP) motifs. In order to better elucidate the immune mechanisms of the Prx2 in the lower vertebrates, we conducted a research about the Prx2 gene of miiuy croaker and its expression pattern after bacterial infection. Real-time PCR (RT-PCR) results showed that expression of Prx2 was up-regulated in kidney, liver and spleen under infection with Vibrio anguillarum, and expressed level differently in ten different uninjected tissues. Our results suggested that Prx2 might be involved in immune defence in miiuy croaker.
authors.affiliation: Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan 316000, PR China.
authors.forename: Liping
authors.initials: L
authors.lastname: Ren
authors.forename: Tianjun
authors.initials: T
authors.lastname: Xu
authors.forename: Rixin
authors.initials: R
authors.lastname: Wang
authors.forename: Yuena
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D005393:Fish Diseases / Q000235:genetics* / Q000382:microbiology*; D040461:Gene Components; D016678:Genome / Q000235:genetics*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D010473:Perciformes; D054464:Peroxiredoxins / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013045:Species Specificity; D013154:Spleen / Q000378:metabolism; D014735:Vibrio Infections / Q000235:genetics / Q000662:veterinary*
chemical_list: D017931:DNA Primers; D054464:Peroxiredoxins