title: Disruption of thiamine uptake and growth of cells by feline leukemia virus subgroup A.
abstract: Feline leukemia virus (FeLV) is still a major cause of morbidity and mortality in domestic cats and some wild cats despite the availability of relatively effective vaccines against the virus. FeLV subgroup A (FeLV-A) is transmitted in natural infections, and FeLV subgroups B, C, and T can evolve directly from FeLV-A by mutation and/or recombination with endogenous retroviruses in domestic cats, resulting in a variety of pathogenic outcomes. The cell surface entry receptor for FeLV-A is a putative thiamine transporter (THTR1). Here, we have addressed whether FeLV-A infection might disrupt thiamine uptake into cells and, because thiamine is an essential nutrient, whether this disruption might have pathological consequences. First, we cloned the cat ortholog of the other of the two known thiamine transporters in mammals, THTR2, and we show that feline THTR1 (feTHTR1) and feTHTR2 both mediate thiamine uptake, but feTHTR2 does not function as a receptor for FeLV-A. We found that feTHTR1 is widely expressed in cat tissues and in cell lines, while expression of feTHTR2 is restricted. Thiamine uptake mediated by feTHTR1 was indeed blocked by FeLV-A infection, and in feline fibroblasts that naturally express feTHTR1 and not feTHTR2, this blockade resulted in a growth arrest at physiological concentrations of extracellular thiamine. The growth arrest was reversed at high extracellular concentrations of thiamine. Our results show that FeLV-A infection can indeed disrupt thiamine uptake with pathological consequences. A prediction of these experiments is that raising the plasma levels of thiamine in FeLV-infected cats may ameliorate the pathogenic effects of infection.
authors.affiliation: Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
authors.forename: Ramon
authors.initials: R
authors.lastname: Mendoza
authors.forename: A Dusty
authors.initials: AD
authors.lastname: Miller
authors.forename: Julie
authors.initials: J
authors.lastname: Overbaugh
mesh_terms: D000818:Animals; D001692:Biological Transport; D002415:Cats; D002460:Cell Line; D049109:Cell Proliferation; D005256:Leukemia Virus, Feline / Q000235:genetics / Q000502:physiology*; D026901:Membrane Transport Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D012192:Retroviridae Infections / Q000821:virology; D013831:Thiamine / Q000378:metabolism*; D014412:Tumor Virus Infections / Q000821:virology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism; D053585:Virus Attachment; D053586:Virus Internalization
chemical_list: D026901:Membrane Transport Proteins; D011991:Receptors, Virus; D014759:Viral Envelope Proteins; D013831:Thiamine