title: Antiplasmodial activity of cucurbitacin glycosides from Datisca glomerata (C. Presl) Baill.
abstract: The traditionally used antimalarial plant, Datisca glomerata (C. Presl) Baill, was subjected to antiplasmodial assay guided fractionation. This led to the isolation of seven cucurbitacin glycosides, datiscosides I-O, along with two known compounds, datiscoside and datiscoside B, from the aerial parts of D. glomerata. Their structures and relative stereochemistry were determined on the basis of mass spectrometry, 1D and 2D NMR spectroscopic data. Antiplasmodial IC(50) values were determined for all isolated compounds against a chloroquine sensitive strain of Plasmodium falciparum (D10), which were also evaluated in vitro for their antileishmanial activity against Leishmania tarentolae. Cytotoxicity was evaluated against rat skeletal muscle cells (L6) and Chinese ovarian hamster cells (CHO). The antiplasmodial activity of the compounds was moderate and ranged from 7.7 to 33.3 μM. None of the compounds showed appreciable antileishmanial activity. The compounds displayed cytotoxicity against L6 but not CHO mammalian cells.
authors.affiliation: Department of Plant Biology and Pathology, SEBS, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA. Rgraz@Eden.Rutgers.edu
authors.forename: Rocky
authors.initials: R
authors.lastname: Graziose
authors.forename: Mary H
authors.initials: MH
authors.lastname: Grace
authors.forename: Thirumurugan
authors.initials: T
authors.lastname: Rathinasabapathy
authors.forename: Patricio
authors.initials: P
authors.lastname: Rojas-Silva
authors.forename: Carmen
authors.initials: C
authors.lastname: Dekock
authors.forename: Alexander
authors.initials: A
authors.lastname: Poulev
authors.forename: Mary Ann
authors.initials: MA
authors.lastname: Lila
authors.forename: Peter
authors.initials: P
authors.lastname: Smith
authors.forename: Ilya
authors.initials: I
authors.lastname: Raskin
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000737:chemistry / Q000494:pharmacology*; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology*; D019684:Magnoliopsida / Q000737:chemistry*; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D010963:Plasmodium falciparum / Q000187:drug effects; D051381:Rats; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000962:Antimalarials; D006027:Glycosides; D014315:Triterpenes