title: [Influence of silencing Notch1 on proliferation, migration and invasion of BGC-823 gastric cancer cells].
abstract: OBJECTIVE
  To investigate the role of Notch1 in the proliferation, migration and invasion of gastric cancer cells.
  
  
  METHODS
  Notch1 expression was detected by RT-PCR and Western blot in four gastric cancer cell lines(SGC-7901, BGC-823, MKN-45 and MKN-28). The RNAi segments targeting Notch1 were designed and transfected into cell lines with the highest level of Notch1. The transcription and translation level of Notch1 expression was detected by real-time PCR and Western blot. The proliferation of transfected cells were determined by CCK8 test. Meanwhile, the migration and invasive ability of transfected tumor cells were detected by Transwell test.
  
  
  RESULTS
  Gastric cell line BGC-823 was selected with the highest expression of Notch1 at both mRNA level and protein level. The expression of Notch1 was significantly reduced after the transfection. The CCK8 test showed the growth rate was significantly reduced in RNAi group after two days(F=4.644, P=0.046). In migration test, the number of cells crossing through chambers in RNAi group(30.1±3.1) was significantly lower than that in the negative control group(44.5±3.2) and in the mock group(46.1±2.6)(P<0.05). In invasion test, the number of cells crossing through chambers in RNAi group(22.9±4.5) was also significantly lower than that in the negative control group (37.3±4.2) and in the mock group (37.8±3.7)(P<0.05).
  
  
  CONCLUSIONS
  Down-regulation of Notch1 inhibits the proliferation and reduces the ability of migration and invasion in gastric cell line BGC-823, suggesting that Notch1 may play an important role in the gastric carcinogenesis and metastasis.
authors.affiliation: Department of General Surgery, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: En-hao
authors.initials: EH
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Shen
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Cao
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D006801:Humans; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D012333:RNA, Messenger; D060888:Real-Time Polymerase Chain Reaction; D051881:Receptor, Notch1 / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms; D014162:Transfection
chemical_list: C497761:NOTCH1 protein, human; D012333:RNA, Messenger; D051881:Receptor, Notch1