title: Induction of apoptosis by chitosan/HPV16 E7 siRNA complexes in cervical             cancer cells.
abstract: Consecutive expression of the high‑risk human papillomavirus (HPV) oncoproteins,             E6 and E7, is pivotal for malignant transformation and maintenance of the malignant             phenotype. These oncogenes may be potential targets of gene silencing‑based molecular             therapies for human cervical cancer. The aim of the present study was to evaluate             the efficacy of chitosan‑based HPV16 E7 siRNA delivery and the chitosan/HPV16 E7             siRNA complex in the induction of apoptosis in CaSki cells constitutively expressing             HPV16 E6 and E7. Chitosan/siRNA nanoparticles were prepared by adding a chitosan             solution drop‑wise to an equal volume of siRNA solution. Formation of the chitosan/siRNA             complex was verified by gel retardation assays and the entry of siRNA into the             cells was confirmed by fluorescence microscopy. Expression of HPV16 E7 was examined             by western blot analysis and apoptotic cells were detected by TUNEL staining.             Chitosan formed complexes with HPV16 E7 siRNA. The chitosan/siRNA nanoparticles             were efficiently delivered into CaSki cells and were observed to induce apoptosis.             In conclusion, chitosan is suitable for use as a carrier for delivery of siRNA             into cancer cells. The delivery of chitosan/HPV16 E7 siRNA nanoparticles in vivo             may serve as a promising therapy for cervical cancer.
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xinxiang Medical College, Henan 453100, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Shaoping
authors.initials: S
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Guo
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yonglian
authors.initials: Y
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Pan
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D005260:Female; D052162:Human papillomavirus 16 / Q000378:metabolism; D006801:Humans; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism; D050725:Papillomavirus E7 Proteins / Q000235:genetics / Q000378:metabolism*; D010316:Particle Size; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry / Q000494:pharmacology*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000473:pathology
chemical_list: D004337:Drug Carriers; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D034741:RNA, Small Interfering; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16; D048271:Chitosan