title: Effects of dietary supplementation of Lactobacillus pentosus PL11 on the growth performance, immune and antioxidant systems of Japanese eel Anguilla japonica challenged with Edwardsiella tarda.
abstract: The aim of this study was to determine the efficacy of dietary administration of Lactobacillus pentosus PL11 on growth performance and the immune and antioxidant systems in Japanese eel Anguilla japonica challenged with Edwardsiella tarda. A total of 75 Japanese eels (24.63±0.83 g) were grouped into 5 treatment diets which were a control diet (C) without E. tarda and 4 treatment diets with E. tarda challenge, including C for E. tarda challenge (NC), C plus L. pentosus PL11 supplemented diet (10⁸ cfu g⁻¹) (T-PL11), C plus L. pentosus KCCM 40997 supplemented diet (10⁸ cfu g⁻¹) (T-Lp) and C plus Weissella hellenica DS-12 supplemented diet (10⁸ cfu g⁻¹) (T-Wh) for 5 weeks (4 week before and 1 week after challenge). The results showed enhanced growth performance in fish fed the diet containing L. pentosus PL11 compared to others. The growth performance parameters including specific growth rate (SGR) and weight gain (WG), feed intake (FI), feed conversion ratio (FCR) and survival were significantly (P<0.05) higher in fish maintained on L. pentosus PL11 supplemented diet compared to C and NC. T-PL11 group also shows a significant increase in the levels of plasma immunoglobulin M, CAT and SOD activities compared to NC. Hematological parameters and mieloperoxidase were significantly better in fish fed the L. pentosus PL11 supplemented diet than in the control. L. pentosus PL11 supplementation recover the reduced expression of SOD, CAT and heat shock protein 70 genes in liver and intestine in pathogen challenged fishes. In conclusion the result of the current study demonstrated L. pentosus PL11 potential as an alternative to antibiotic supplementation to improve the growth and health performance of Japanese eel (A. japonica).
authors.affiliation: Laboratory of Clinical Pharmacokinetics and Pharmacodynamics, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Joong-Su
authors.initials: JS
authors.lastname: Lee
authors.forename: Henrique
authors.initials: H
authors.lastname: Cheng
authors.forename: Dereje
authors.initials: D
authors.lastname: Damte
authors.forename: Seung-Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Jong-Choon
authors.initials: JC
authors.lastname: Kim
authors.forename: Man-Hee
authors.initials: MH
authors.lastname: Rhee
authors.forename: Joo-Woon
authors.initials: JW
authors.lastname: Suh
authors.forename: Seung-Chun
authors.initials: SC
authors.lastname: Park
mesh_terms: D000811:Anguilla / Q000254:growth & development* / Q000276:immunology* / Q000382:microbiology; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004032:Diet / Q000662:veterinary; D020609:Edwardsiella tarda / Q000502:physiology; D004756:Enterobacteriaceae Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology; D007113:Immunity, Innate; D007778:Lactobacillus / Q000302:isolation & purification / Q000378:metabolism*; D019936:Probiotics / Q000008:administration & dosage
chemical_list: D000975:Antioxidants