title: Using genetic diversity information to establish core collections of Stylosanthes capitata and Stylosanthes macrocephala.
abstract: Stylosanthes species are important forage legumes in tropical and subtropical areas. S. macrocephala and S. capitata germplasm collections that consist of 134 and 192 accessions, respectively, are maintained at the Brazilian Agricultural Research Corporation Cerrados (Embrapa-Cerrados). Polymorphic microsatellite markers were used to assess genetic diversity and population structure with the aim to assemble a core collection. The mean values of H(O) and H(E) for S. macrocephala were 0.08 and 0.36, respectively, whereas the means for S. capitata were 0.48 and 0.50, respectively. Roger's genetic distance varied from 0 to 0.83 for S. macrocephala and from 0 to 0.85 for S. capitata. Analysis with STRUCTURE software distinguished five groups among the S. macrocephala accessions and four groups among those of S. capitata. Nei's genetic diversity was 27% in S. macrocephala and 11% in S. capitata. Core collections were assembled for both species. For S. macrocephala, all of the allelic diversity was represented by 23 accessions, whereas only 13 accessions were necessary to represent all allelic diversity for S. capitata. The data presented herein evidence the population structure present in the Embrapa-Cerrados germplasm collections of S. macrocephala and S. capitata, which may be useful for breeding programs and germplasm conservation.
authors.affiliation: Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, SP, Brazil.
authors.forename: Melissa Oliveira
authors.initials: MO
authors.lastname: Santos-Garcia
authors.forename: Guilherme
authors.initials: G
authors.lastname: de Toledo-Silva
authors.forename: Rodrigo Possidonio
authors.initials: RP
authors.lastname: Sassaki
authors.forename: Thais Helena
authors.initials: TH
authors.lastname: Ferreira
authors.forename: Rosângela Maria Simeão
authors.initials: RM
authors.lastname: Resende
authors.forename: Lucimara
authors.initials: L
authors.lastname: Chiari
authors.forename: Cláudio Takao
authors.initials: CT
authors.lastname: Karia
authors.forename: Marcelo Ayres
authors.initials: MA
authors.lastname: Carvalho
authors.forename: Fábio Gelape
authors.initials: FG
authors.lastname: Faleiro
authors.forename: Maria Imaculada
authors.initials: MI
authors.lastname: Zucchi
authors.forename: Anete Pereira
authors.initials: AP
authors.lastname: de Souza