title: Synthesis and in vitro release study of ibuprofen-loaded gelatin graft copolymer nanoparticles.
abstract: OBJECTIVE
  This work deals with the preparation, characterization and in vitro release study of IBU-loaded gel graft copolymer nanoparticles.
  
  
  METHODS
  Gelatin (Gel) graft copolymer nanoparticles were prepared using styrene (Sty) and/or 2-hydroxyethyl methacrylate (HEMA) monomers in the presence of potassium persulfate and glutaraldehyde as an initiator and cross-linker, respectively. The prepared nanoparticles as sustained release drug carriers were investigated using the nonsteriodal anti-inflammatory model drug, ibuprofen (IBU).
  
  
  RESULTS
  The prepared nanoparticles as sustained release drug carriers were investigated using the nonsteriodal anti-inflammatory model drug, IBU. The prepared Gel/HEMA and Gel/Sty nanoparticles exhibited particles size ranging from 15 to 17 nm and from 0.42 to 5 mm, respectively. The dissolution of IBU in phosphate buffer, pH 7.4, at 37°C from the prepared nanoparticles was evaluated using UV spectroscopy. In addition, the prepared nanoparticles were characterized using Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), transmitting electron microscope (TEM) and zeta potential/particle size analyzer. In vitro dissolution study showed that the dissolution rates of the crosslinked nanoparticles were retarded relative to the uncrosslinked ones. Moreover, the released amount constantly decreases with increasing gluteraldehyde content in the gel nanoparticles.
  
  
  CONCLUSIONS
  Crosslinked gel-based graft copolymers exhibited slow IBU release within six hours. Furthermore, results from different characterization techniques such as TEM, particles size and zeta potential measurements confirmed the formation of pH-responsive gel-graft copolymer nanoparticles.
authors.affiliation: Chemical Industries Research Division.
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Haroun
authors.forename: N R
authors.initials: NR
authors.lastname: El-Halawany
authors.forename: C
authors.initials: C
authors.lastname: Loira-Pastoriza
authors.forename: P
authors.initials: P
authors.lastname: Maincent
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000737:chemistry; D002152:Calorimetry, Differential Scanning; D003432:Cross-Linking Reagents / Q000737:chemistry; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding; D005780:Gelatin / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007052:Ibuprofen / Q000008:administration & dosage* / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry; D053758:Nanoparticles; D010316:Particle Size; D011108:Polymers / Q000737:chemistry; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D020058:Styrene / Q000737:chemistry; D013997:Time Factors
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D003432:Cross-Linking Reagents; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D008689:Methacrylates; D011108:Polymers; D020058:Styrene; C005044:hydroxyethyl methacrylate; D005780:Gelatin; D007052:Ibuprofen