title: Synthesis of DNA oligonucleotides containing C5-ethynylbenzenesulfonamide-modified nucleotides (EBNA) by polymerases towards the construction of base functionalized nucleic acids.
abstract: C5-Ethynylbenzenesulfonamide-modified nucleotide (EBNA) was investigated as substrate of various DNA polymerases. The experiments revealed that KOD, Phusion and Klenow DNA polymerases successfully accepted EBNA-T nucleotide as a substrate and yielded the fully extended DNA. KOD DNA polymerase was found to be the most efficient enzyme to furnish EBNA-T containing DNA in good yields. Phusion DNA polymerase efficiently amplified the template containing EBNA-T nucleotides by PCR.
authors.affiliation: School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Brisbane 4072, Australia.
authors.forename: Astrid
authors.initials: A
authors.lastname: Goubet
authors.forename: Antoine
authors.initials: A
authors.lastname: Chardon
authors.forename: Pawan
authors.initials: P
authors.lastname: Kumar
authors.forename: Pawan K
authors.initials: PK
authors.lastname: Sharma
authors.forename: Rakesh N
authors.initials: RN
authors.lastname: Veedu
mesh_terms: D000114:Acetylene / Q000737:chemistry*; D001483:Base Sequence; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009696:Nucleic Acids / Q000378:metabolism; D009711:Nucleotides / Q000378:metabolism; D009841:Oligonucleotides / Q000138:chemical synthesis*; D016133:Polymerase Chain Reaction; D013379:Substrate Specificity; D013449:Sulfonamides / Q000737:chemistry*; D000096926:Benzenesulfonamides
chemical_list: D000114:Acetylene; D004259:DNA-Directed DNA Polymerase; D009696:Nucleic Acids; D009711:Nucleotides; D009841:Oligonucleotides; D013449:Sulfonamides; D000096926:Benzenesulfonamides