title: Change in metabolic status of glutathione by palladium nitrate in blood components.
abstract: This piece of research work present the toxicological impact of varied concentrations of palladium nitrate [Pd (NO3)2] by changing the chemical status of glutathione and the way how glutathione plays its role in detoxification and conjugation processes of [Pd (NO(3))(2))] in whole blood components (plasma and cytosolic fraction). The impact of different concentration of [Pd (NO3)2] on reduced glutathione level in whole blood component (plasma and cytosolic fraction) were measured spectrophotometrically following Standard Ellman's method. Compared with control sample, significant decrease in the GSH content in whole blood components (plasma and cytosolic fraction) was obtained with various concentrations (100µM-1000µM) of palladium nitrate. Depleted GSH level was more pronounced with time incubation period (0-90) minutes. These finding shows that changes in the GSH status produced by palladium nitrate could either be due to palladium nitrate and glutathione( Pd-SG) complex formation or by conversion of reduce glutathione (2GSH + Pd(+2) - GSSG). This change in the GSH metabolic status provides information regarding the mechanism of palladium, in blood components.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gomal University, D I Khan, KPK.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Mukhtiar
authors.forename: Muhammad Farid
authors.initials: MF
authors.lastname: Khan
authors.forename: Syed Umer
authors.initials: SU
authors.lastname: Jan
authors.forename: Haroon
authors.initials: H
authors.lastname: Khan
authors.forename: Naseem
authors.initials: N
authors.lastname: Ullah
authors.forename: Amir
authors.initials: A
authors.lastname: Badshah
mesh_terms: D003600:Cytosol / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000097:blood*; D006801:Humans; D010165:Palladium / Q000097:blood / Q000633:toxicity*; D013053:Spectrophotometry; D013997:Time Factors
chemical_list: C090639:palladium nitrate; D010165:Palladium; D005978:Glutathione