title: Impact of bone marrow-derived mesenchymal stromal cells on experimental xenogeneic graft-versus-host disease.
abstract: OBJECTIVE
  Graft-versus-host disease (GVHD) is a life-threatening complication of allogeneic hematopoietic cell transplantation caused by donor T cells reacting against host tissues. Previous studies have suggested that mesenchymal stromal cells (MSCs) could exert potent immunosuppressive effects.
  
  
  METHODS
  The ability of human bone marrow derived MSCs to prevent xenogeneic GVHD in non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice and in NOD/SCID/interleukin-2Rγ(null) (NSG) mice transplanted with human peripheral blood mononuclear cells (PBMCs) was assessed.
  
  
  RESULTS
  Injection of 200 × 10(6) human PBMCs intraperitoneally (IP) into sub-lethally (3.0 Gy) irradiated NOD/SCID mice also given anti-asialo GM1 antibodies IP 1 day prior and 8 days after transplantation induced lethal xenogeneic GVHD in all tested mice. Co-injection of 2 × 10(6) MSCs IP on day 0 did not prevent lethal xenogeneic GVHD induced by injection of human PBMCs. Similarly, injection of 30 × 10(6) human PBMCs IP into sub-lethally (2.5 Gy) irradiated NSG mice induced a lethal xenogeneic GVHD in all tested mice. Injection of 3 × 10(6) MSCs IP on days 0, 7, 14 and 21 did not prevent lethal xenogeneic GVHD induced by injection of human PBMCs.
  
  
  CONCLUSIONS
  Injection of MSCs did not prevent xenogeneic GVHD in these two humanized mice models.
authors.affiliation: Groupe Interdisciplinaire de Génoprotéomique Appliquée (GIGA)-I(3), University of Liège, Liège, Belgium.
authors.forename: France
authors.initials: F
authors.lastname: Bruck
authors.forename: Ludovic
authors.initials: L
authors.lastname: Belle
authors.forename: Chantal
authors.initials: C
authors.lastname: Lechanteur
authors.forename: Laurence
authors.initials: L
authors.lastname: de Leval
authors.forename: Muriel
authors.initials: M
authors.lastname: Hannon
authors.forename: Sophie
authors.initials: S
authors.lastname: Dubois
authors.forename: Emilie
authors.initials: E
authors.lastname: Castermans
authors.forename: Stephanie
authors.initials: S
authors.lastname: Humblet-Baron
authors.forename: Souad
authors.initials: S
authors.lastname: Rahmouni
authors.forename: Yves
authors.initials: Y
authors.lastname: Beguin
authors.forename: Alexandra
authors.initials: A
authors.lastname: Briquet
authors.forename: Frédéric
authors.initials: F
authors.lastname: Baron
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D006086:Graft vs Host Disease / Q000209:etiology / Q000235:genetics / Q000503:physiopathology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000637:transplantation; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D016513:Mice, SCID; D013601:T-Lymphocytes / Q000276:immunology / Q000637:transplantation; D014184:Transplantation, Homologous / Q000009:adverse effects