title: Protective effect of methanolic extract of Garcinia indica fruits in 6-OHDA rat model of Parkinson's disease.
abstract: BACKGROUND
  Several studies have reported that antioxidants play an important role in Parkinson's disease (PD). Garcinia indica extract is a natural antioxidant, the present study was undertaken to evaluate the neuroprotective effect of methanolic extract of Garcinia indica (GIM) against 6-hydroxydopamine (6-OHDA) neurotoxicity for striatal dopaminergic neurons in the rat.
  
  
  METHODS
  Thirty adult Wistar rats were randomly divided into five groups namely control, 6-OHDA model, and GIM (100, 200, and 400 mg/kg body weight suspended in one ml of 0.1% carboxymethyl cellulose). The treatment was started three days before surgery and continued for next 14 days. The surgery was done on third day in all groups for administration of 6-OHDA into the right striatum and right substantia nigra, whereas control group injected with 6-OHDA vehicle. Various behavior and biochemical tests (Apomorphine-induced rotational behavior, Stepping test, Initiation time, Postural balance test, and Disengage time) were used to evaluate the neuroprotective effect of GIM. One-way analysis of variance (ANOVA) followed by Dunnett's test was used to compare inter-group differences. P<0.05 was considered as statistically significant.
  
  
  RESULTS
  GIM had significant (P<0.05, P<0.01) preventive effect in biochemical tests, i.e., dopamine and its metabolites measurement and in various behavior tests, i.e., apomorphine-induced rotational behavior, stepping test, initiation time, postural balance test, and disengage time as compared to 6-OHDA-treated rats.
  
  
  CONCLUSIONS
  Our results demonstrated that GIM acted as an effective neuroprotective agent for striatal dopaminergic neurons in 6-OHDA lesioned rat model of PD.
authors.affiliation: Department of Pharmacology and Toxicology, NIPER, Guwahati, Assam, India.
authors.forename: Bhaveshkumar V
authors.initials: BV
authors.lastname: Antala
authors.forename: Manishkumar S
authors.initials: MS
authors.lastname: Patel
authors.forename: Satish V
authors.initials: SV
authors.lastname: Bhuva
authors.forename: Shiv
authors.initials: S
authors.lastname: Gupta
authors.forename: Samir
authors.initials: S
authors.lastname: Rabadiya
authors.forename: Mangala
authors.initials: M
authors.lastname: Lahkar
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism; D005638:Fruit; D029760:Garcinia; D006719:Homovanillic Acid / Q000378:metabolism; D008297:Male; D000432:Methanol / Q000737:chemistry; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D016627:Oxidopamine; D020734:Parkinsonian Disorders / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents / Q000737:chemistry
chemical_list: D018696:Neuroprotective Agents; D010936:Plant Extracts; D012997:Solvents; D015102:3,4-Dihydroxyphenylacetic Acid; D016627:Oxidopamine; D004298:Dopamine; D006719:Homovanillic Acid; D000432:Methanol