title: Identification of dynamin as a septin-binding protein.
abstract: Lipid rafts (detergent-resistant low-density membrane microdomain: DRM) are signal-transducing membrane platforms. In a previous study, we showed maturation-dependent localization of septin in the DRM fraction of rat brain. Mammalian septin is composed with 13-14 isoforms and these isoforms assemble to form rod-shaped hetero-oligomeric complexes. End-to-end polymerization of these complexes results in the formation of higher order structures such as filamentous sheets or bundles of filaments that restrict the fluid-like diffusion of the membrane proteins and lipids. Considering the function of septin as the membrane scaffold, elucidation of the molecular interaction of septin in DRM could be a breakthrough to understand another role of lipid rafts. In order to identify septin-binding proteins in DRM, solubilization and fractionation of septin from DRM was attempted. Several proteins were co-fractionated with septin and LC-MS/MS analysis identified one of these proteins as dynamin and Western blotting using anti-dynamin confirmed this result. Immunoprecipitation of septin11 in a crude supernatant showed co-precipitation of dynamin and dynamin fraction prepared from brain contained several septin isoforms. Within bacterially expressed septin isoforms, septin5 and septin11 bound dynamin but septin9 did not. These results suggest that some septin isoforms participate in the dynamin-related membrane dynamics.
authors.affiliation: Department of Biology, Graduate School of Science, Kobe-University, Kobe 657-8501, Japan.
authors.forename: Maowulan
authors.initials: M
authors.lastname: Maimaitiyiming
authors.forename: Yuumi
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Haruko
authors.initials: H
authors.lastname: Kumanogoh
authors.forename: Shun
authors.initials: S
authors.lastname: Nakamura
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Morita
authors.forename: Shohei
authors.initials: S
authors.lastname: Maekawa
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D034281:Dynamins / Q000737:chemistry* / Q000378:metabolism; D021962:Membrane Microdomains / Q000737:chemistry; D011485:Protein Binding; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry; D058112:Septins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D020033:Protein Isoforms; D011994:Recombinant Proteins; D058112:Septins; D034281:Dynamins