title: Systemic immune modulation induced by alcoholic beverage intake in obese-diabetes (db/db) mice.
abstract: Alcohol over-consumption is generally immunosuppressive. In this study, the effects of single or repetitive alcohol administration on the systemic immunity of db/db mice were observed to clarify the possible mechanisms for the increased susceptibility of obese individuals to alcohol-related immunological health problems. Alcohol (as a form of commercially available 20% distilled-alcoholic beverage) was orally administered one-time or seven times over 2 weeks to db/db mice and normal C57BL/6J mice. Immunologic alterations were analyzed by observation of body weight and animal activity, along with proportional changes of splenocytes for natural killer cells, macrophages, and T and B lymphocytes. Modulation of plasma cytokine level and immune-related genes were also ascertained by micro-bead assay and a microarray method, respectively. The immune micro-environment of db/db mice was an inflammatory state and adaptive cellular immunity was significantly suppressed. Low-dose alcohol administration reversed the immune response, decreasing inflammatory responses and the increment of adaptive immunity mainly related to CD4(+) T cells, but not CD8(+) T cells, to normal background levels. Systemic immune modulation due to alcohol administration in the obese-diabetic mouse model may be useful in the understanding of the induction mechanism, which will aid the development of therapeutics for related secondary diseases.
authors.affiliation: Office of Biomedical Professors, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Il-Won Dong, Kang-Nam Gu, Seoul 135-710, Republic of Korea. andyjosh@skku.edu
authors.forename: Hyunah
authors.initials: H
authors.lastname: Lee
authors.forename: Ik-Soon
authors.initials: IS
authors.lastname: Jang
authors.forename: Junsoo
authors.initials: J
authors.lastname: Park
authors.forename: Seol-Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: So-Young
authors.initials: SY
authors.lastname: Baek
authors.forename: Sung-Ho
authors.initials: SH
authors.lastname: Go
authors.forename: Seung-Hoon
authors.initials: SH
authors.lastname: Lee
mesh_terms: D056704:Adaptive Immunity; D000434:Alcoholic Beverages / Q000009:adverse effects*; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002460:Cell Line; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism; D007111:Immunity, Cellular / Q000276:immunology*; D007113:Immunity, Innate; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D010641:Phenotype
chemical_list: D016207:Cytokines