title: Molecular character of a phosphatase 2C (PP2C) gene relation to stress tolerance in Arabidopsis thaliana.
abstract: Protein phosphatases type 2C (PP2Cs) from group A, which includes the ABI1/HAB1 and PP2CA branches, are key negative regulators of ABA signaling. HAI-1 gene had been shown to affect both seed and vegetative responses to ABA, which is one of PP2Cs clade A in Arabidopsis thaliana. Transgenic plants containing pHAI-1::GUS (β-glucuronidase) displayed GUS activity existing in the vascular system of leave veins, stems and petioles. Green fluorescent protein fused HAI-1 (HAI-1-GFP) was found in the nucleus through transient transformation assays with onion epidermal cells. The water-loss assays indicated the loss-of-function mutants did not show symptoms of wilting and they had still turgid green rosette leaves. The assays of seed germination by exogenous ABA and NaCl manifested that the loss-of-function mutants displayed higher insensitivity than wild-type plants. Taken together, the final results suggest that the HAI-1 (AT5G59220) encoded a nuclear protein and it can be highly induced by ABA and wound in Arabidposis, the stress-tolerance phenotype showed a slightly improvement when HAI-1 gene was disrupted.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha, 410082, China.
authors.forename: Jihong
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiushan
authors.initials: X
authors.lastname: Li
authors.forename: Zhimin
authors.initials: Z
authors.lastname: He
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhao
authors.forename: Qiming
authors.initials: Q
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.forename: Dashi
authors.initials: D
authors.lastname: Yu
authors.forename: Xinqun
authors.initials: X
authors.lastname: Huang
authors.forename: Dongying
authors.initials: D
authors.lastname: Tang
authors.forename: Xinhong
authors.initials: X
authors.lastname: Guo
authors.forename: Xuanming
authors.initials: X
authors.lastname: Liu
mesh_terms: D000220:Adaptation, Biological / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D023061:Gene Order; D005822:Genetic Vectors; D009154:Mutation; D010641:Phenotype; D010749:Phosphoprotein Phosphatases / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified; D021381:Protein Transport; D055049:Salt Tolerance / Q000235:genetics; D013312:Stress, Physiological
chemical_list: D010749:Phosphoprotein Phosphatases