title: Clinical impact of PAI 1 4G/5G gene polymorphism in colorectal carcinoma patients.
abstract: Plasminogen activator ihnibitor (PAI 1) belongs to the plasminogen activator system, which is part of the metastatic cascade and significantly contributes to invasive growth and angiogenesis of malignant tumors. Its plasma level is normally low but 4G/4G homozygotes have higher concentrations of PAI 1. This genotype may be associated with worse prognosis and proximal location of colorectal cancer than 5G/5G homozygotes. In our prospective evaluation we examined plasma level PAI 1 (using photometric microplate method ELISA) pre-surgery and, subsequently, 6-8 weeks later, from 80 patients. For the PAI 1 rs1799889 -675 4G/5G polymorphism test the PCR amplification was used.Analysis of collected data was confirmed that significantly higher plasma levels of PAI 1 were found in patients before starting therapy, which decreased (p=0.004) after initiation of treatment. Patients with higher plasma level PAI 1 before (p=0.013) and after therapy (p=0.004) had significantly shorter survival. We found no relationship between polymorphisms of PAI 1 (-675 4G/5G) in relation to stage, survival or tumor location. PAI 1 is useful as a negative marker of prognosis and could be advantageous when planning adjuvant treatment of patients with colorectal carcinoma. Although opinions on the importance of polymorphisms of PAI 1 in relation to the prognosis are not uniform, it does seem that their role in the prognosis of patients with colorectal cancer is not essential.
authors.affiliation: Department of Complex Oncology Care, Masaryk University, Brno, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Halamkova
authors.forename: I
authors.initials: I
authors.lastname: Kiss
authors.forename: Z
authors.initials: Z
authors.lastname: Pavlovsky
authors.forename: J
authors.initials: J
authors.lastname: Tomasek
authors.forename: J
authors.initials: J
authors.lastname: Jarkovsky
authors.forename: Z
authors.initials: Z
authors.lastname: Cech
authors.forename: D
authors.initials: D
authors.lastname: Bednarova
authors.forename: S
authors.initials: S
authors.lastname: Tucek
authors.forename: L
authors.initials: L
authors.lastname: Hanakova
authors.forename: M
authors.initials: M
authors.lastname: Moulis
authors.forename: J
authors.initials: J
authors.lastname: Zavrelova
authors.forename: M
authors.initials: M
authors.lastname: Man
authors.forename: P
authors.initials: P
authors.lastname: Benda
authors.forename: O
authors.initials: O
authors.lastname: Robek
authors.forename: Z
authors.initials: Z
authors.lastname: Kala
authors.forename: M
authors.initials: M
authors.lastname: Penka
mesh_terms: D015179:Colorectal Neoplasms / Q000235:genetics* / Q000401:mortality; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D017395:Plasminogen Activator Inhibitor 1