title: A novel mouse model that closely mimics human uterine leiomyomas.
abstract: OBJECTIVE
  To develop a predictive mouse model for uterine fibroids.
  
  
  METHODS
  Human fibroid cells xenografted to immunodeficient mice.
  
  
  METHODS
  University and industrial research center.
  
  
  METHODS
  Immunodeficient scid/beige mice.
  
  
  METHODS
  Subcutaneous and intrauterine injection of fibroid-derived cells, SV40 transformation of primary cells by lentiviral transduction, proliferation determined by immunohistochemistry, FISH.
  
  
  METHODS
  Characterization of primary and immortalized cells by Western blot and soft agar assay, determination of in vivo tumorigenicity, comparative histology and immunohistochemistry, fluorescence in situ hybridization.
  
  
  RESULTS
  Tumorigenicity of primary myoma cells disappears upon in vitro culture. Transformation and immortalization does not restore or conserve the in vivo growth potential of cultured cells. Injection of primary cells into myometrium of mice leads to xenografts with a leiomyoma-like histology.
  
  
  CONCLUSIONS
  Primary myoma cells are suited to generate fibroid-like xenografts for studying pathogenesis without genetic modifications. In contrast, in vitro culture abolishes transplantability, and neither transformation nor immortalization is sufficient to restore tumorigenic capacity.
authors.affiliation: Center of Human Genetics, University of Bremen, Bremen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Drosch
authors.forename: Jörn
authors.initials: J
authors.lastname: Bullerdiek
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Zollner
authors.forename: Florian
authors.initials: F
authors.lastname: Prinz
authors.forename: Markus
authors.initials: M
authors.lastname: Koch
authors.forename: Nicole
authors.initials: N
authors.lastname: Schmidt
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology; D000818:Animals; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D007889:Leiomyoma / Q000473:pathology* / Q000503:physiopathology; D008175:Lung Neoplasms / Q000473:pathology; D051379:Mice; D016513:Mice, SCID; D061251:Primary Cell Culture; D014594:Uterine Neoplasms / Q000473:pathology* / Q000503:physiopathology; D023041:Xenograft Model Antitumor Assays