title: Evaluation of the effects of transient or continuous PTH administration to odontoblast-like cells.
abstract: Parathyroid hormone participates in the metabolism of mineralized tissue. Its role in the formation of dentine is, as yet, incompletely understood. In the present study we analyzed the effect of transient (1 and 24-h/cycle) or continuous hPTH (1-34) treatment in odontoblast-like cells (MDPC-23) to the following parameters: mineral deposition detected by alizarin red, mRNA expression of the type I collagen (COL1), alkaline phosphatase (ALP), biglycan (BGN), matrix metalloproteinase 2 (MMP-2) and dentine sialophosphoprotein (DSPP) quantified by qRT-PCR. MMP-2 and ALP activities were quantified by zymography and colorimetric assay, respectively. The results showed that compared to Control group: intermittent PTH administration (1 and 24-h/cycle) decreased the mineral deposition and ALP activity. DSPP gene expression was not detected in both control and PTH treated cells. The PTH administration for 24-h/cycle increased the ALP, BGN and COL1 mRNA expression and continuous PTH treatment increased BGN and COL1 mRNA expression. Zymography assays showed that compared to Control group: PTH treatment for 1-h/cycle increased the total MMP-2 secretion and the continuous treatment decreased the secreted levels of MMP-2 active-form. Taken together, the results shown that PTH may regulate the odontoblast-like cells-induced secretion, and potentially this hormone can affect in vivo odontoblasts functions.
authors.affiliation: Department of Morphology, Division of Histology, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil.
authors.forename: Gustavo Narvaes
authors.initials: GN
authors.lastname: Guimarães
authors.forename: Rafael Nóbrega
authors.initials: RN
authors.lastname: Stipp
authors.forename: Thaisângela Lopes
authors.initials: TL
authors.lastname: Rodrigues
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: de Souza
authors.forename: Sergio Roberto Peres
authors.initials: SR
authors.lastname: Line
authors.forename: Marcelo Rocha
authors.initials: MR
authors.lastname: Marques
mesh_terms: D000469:Alkaline Phosphatase / Q000187:drug effects; D000818:Animals; D000880:Anthraquinones; D058578:Biglycan / Q000187:drug effects; D002113:Calcification, Physiologic / Q000187:drug effects; D018929:Cell Culture Techniques; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D024042:Collagen Type I / Q000187:drug effects; D003124:Colorimetry; D004396:Coloring Agents; D004334:Drug Administration Schedule; D016326:Extracellular Matrix Proteins / Q000187:drug effects; D020778:Matrix Metalloproteinase 2 / Q000187:drug effects; D051379:Mice; D009804:Odontoblasts / Q000187:drug effects*; D010281:Parathyroid Hormone / Q000008:administration & dosage* / Q000494:pharmacology; D010750:Phosphoproteins / Q000187:drug effects; D012333:RNA, Messenger / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000187:drug effects; D013778:Tetrazolium Salts; D013844:Thiazoles; D000099311:Dentin Sialophosphoprotein
chemical_list: D000469:Alkaline Phosphatase; D000880:Anthraquinones; D058578:Biglycan; D024042:Collagen Type I; D004396:Coloring Agents; D016326:Extracellular Matrix Proteins; D020778:Matrix Metalloproteinase 2; D010281:Parathyroid Hormone; D010750:Phosphoproteins; D012333:RNA, Messenger; D012795:Sialoglycoproteins; D013778:Tetrazolium Salts; D013844:Thiazoles; D000099311:Dentin Sialophosphoprotein; C546885:Bgn protein, mouse; C010078:alizarin; C522362:Mmp2 protein, mouse; C022616:thiazolyl blue