title: In vitro propagation of hepatopancreatic parvo-like virus (HPV) of shrimp in C6/36 (Aedes albopictus) cell line.
abstract: Hepatopancreatic parvovirus (HPV) which causes infection in many species of penaeid shrimp is a serious viral pathogen in the young life stages of shrimp. An attempt was made to develop an in vitro system using C6/36 subclone of Aedes albopictus cell line for propagation of HPV. The results revealed that C6/36 cells were susceptible to this virus and the infected cells showed CPE in the form of vacuole formation. The results of PCR, immunocytochemistry and Western blot revealed the HPV-infection in C6/36 cell line. The RT-PCR analysis confirmed the replication of HPV in C6/36 cell line. The HPV load was quantified at different time intervals by ELISA and real time PCR, and the results showed the increase of viral load in C6/36 cell line in time course of infection. HPV propagated in C6/36 cell line was used to infect post-larvae of shrimp and the results showed that the twentieth passage of HPV propagated in C6/36 cell line caused 100% mortality in post-larvae after 6 weeks post infection (d.p.i.). The infected post-larvae showed clinical signs of reduced growth, reduced preening, muscle opacity and atrophy of hepatopancreas. The HPV-infection was confirmed by PCR. The results of the present study showed that C6/36 cell line can be used as an in vitro model for HPV replication instead of whole animal.
authors.affiliation: OIE Reference Laboratory for WTD, Aquaculture Biotechnology Laboratory, PG & Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, Vellore District, Tamil Nadu 632 509, India.
authors.forename: N
authors.initials: N
authors.lastname: Madan
authors.forename: K S N
authors.initials: KS
authors.lastname: Nambi
authors.forename: S
authors.initials: S
authors.lastname: Abdul Majeed
authors.forename: G
authors.initials: G
authors.lastname: Taju
authors.forename: N
authors.initials: N
authors.lastname: Sundar Raj
authors.forename: M A
authors.initials: MA
authors.lastname: Farook
authors.forename: S
authors.initials: S
authors.lastname: Vimal
authors.forename: A S
authors.initials: AS
authors.lastname: Sahul Hameed
mesh_terms: D000330:Aedes; D000818:Animals; D002460:Cell Line; D019223:Densovirinae / Q000502:physiology*; D008954:Models, Biological; D033561:Penaeidae / Q000821:virology*; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication