title: Experimental mixture design as a tool to enhance glycosyl hydrolases production by a new Trichoderma harzianum P49P11 strain cultivated under controlled bioreactor submerged fermentation.
abstract: This work investigates the glycosyl hydrolase (GH) profile of a new Trichoderma harzianum strain cultivated under controlled bioreactor submerged fermentation. The influence of different medium components (delignified steam-exploded sugarcane bagasse, sucrose, and soybean flour) on GH biosynthesis was assessed using experimental mixture design (EMD). Additionally, the effect of increased component concentrations in culture media selected from the EMD was studied. It was found that that a mixed culture medium could significantly maximize GH biosynthesis rate, especially for xylanase enzymes which achieved a 2-fold increment. Overall, it was demonstrated that T. harzianumP49P11 enzymes have a great potential to be used in the deconstruction of biomass.
authors.affiliation: Brazilian Bioethanol Science and Technology Laboratory - CTBE, Rua Giuseppe Maximo Scolfaro 10000, Pólo II de Alta Tecnologia, Caixa Postal 6192, CEP 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Priscila da Silva
authors.initials: Pda S
authors.lastname: Delabona
authors.forename: Cristiane Sanchez
authors.initials: CS
authors.lastname: Farinas
authors.forename: Deise Juliana da Silva
authors.initials: DJ
authors.lastname: Lima
authors.forename: José Geraldo da Cruz
authors.initials: JG
authors.lastname: Pradella
mesh_terms: D056804:Biofuels; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D001938:Brazil; D002482:Cellulose / Q000378:metabolism; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D031786:Saccharum / Q000378:metabolism; D013025:Glycine max / Q000378:metabolism; D013045:Species Specificity; D013395:Sucrose / Q000378:metabolism; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics
chemical_list: D056804:Biofuels; D013395:Sucrose; D002482:Cellulose; C027433:bagasse; D006026:Glycoside Hydrolases