title: LGP2 expression is enhanced by interferon regulatory factor 3 in olive flounder, Paralichthys olivaceus.
abstract: In innate immunity, LGP2 (laboratory of genetics and physiology 2) plays a very important role in the production of type I interferon (IFN) through recognition of cytosolic viral RNA. Although viral infection or stimulation with double-strand RNA dramatically induces expression of the LGP2 gene, the underlying transcriptional mechanism has never been studied. Here, we cloned and characterized the 5'-upstream region (-1,337 bp) of the LGP2 gene in olive flounder (Paralichthys olivaceus). Numerous canonical motifs for IFN-regulatory factors (IRFs) were found in this region, and reporter assays identified a poly I:C-responsive promoter region (-506 to -398) that regulated LGP2 transcription. Transcriptional activity of the LGP2 promoter was strongly enhanced by IRF3, which bound to IRF3 motif #3 (-480). The LGP2 promoter was also responsive to viral infection in vitro. These results suggest that LGP2 transcriptional control is crucially involved to regulated by IRF3 function after viral infection or stimulation with poly I:C.
authors.affiliation: Aquatic Biotechnology Center, College of Veterinary Medicine, Gyeongsang National University, Jinju, Gyeongnam, South Korea.
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Hikima
authors.forename: Mi-Kyong
authors.initials: MK
authors.lastname: Yi
authors.forename: Maki
authors.initials: M
authors.lastname: Ohtani
authors.forename: Chan Yong
authors.initials: CY
authors.lastname: Jung
authors.forename: Young Kyu
authors.initials: YK
authors.lastname: Kim
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Mun
authors.forename: Young Rim
authors.initials: YR
authors.lastname: Kim
authors.forename: Haruko
authors.initials: H
authors.lastname: Takeyama
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoki
authors.forename: Tae Sung
authors.initials: TS
authors.lastname: Jung
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D005393:Fish Diseases / Q000235:genetics / Q000821:virology; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D005432:Flounder / Q000235:genetics* / Q000821:virology; D005786:Gene Expression Regulation / Q000187:drug effects; D050838:Interferon Regulatory Factor-3 / Q000378:metabolism*; D008969:Molecular Sequence Data; D029141:Novirhabdovirus / Q000187:drug effects / Q000502:physiology; D059372:Nucleotide Motifs / Q000235:genetics; D011070:Poly I-C / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding / Q000187:drug effects / Q000235:genetics; D020365:RNA Helicases / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D029941:Fish Proteins; D050838:Interferon Regulatory Factor-3; D020365:RNA Helicases; D011070:Poly I-C