title: Hair as a biomarker of environmental manganese exposure.
abstract: The absence of well-validated biomarkers of manganese (Mn) exposure in children remains a major obstacle for studies of Mn toxicity. We developed a hair cleaning methodology to establish the utility of hair as an exposure biomarker for Mn and other metals (Pb, Cr, Cu), using ICPMS, scanning electron microscopy, and laser ablation ICPMS to evaluate cleaning efficacy. Exogenous metal contamination on hair that was untreated or intentionally contaminated with dust or Mn-contaminated water was effectively removed using a cleaning method of 0.5%Triton X-100 sonication plus 1 N nitric acid sonication. This cleaning method was then used on hair samples from children (n = 121) in an ongoing study of environmental Mn exposure and related health effects. Mean hair Mn levels were 0.121 μg/g (median = 0.073 μg/g, range = 0.011-0.736 μg/g), which are ∼4 to 70-fold lower than levels reported in other pediatric Mn studies. Hair Mn levels were also significantly higher in children living in the vicinity of active, but not historic, ferroalloy plant emissions compared to controls (P < 0.001). These data show that hair can be effectively cleaned of exogenous metal contamination, and they substantiate the use of hair Mn levels as a biomarker of environmental Mn exposure in children.
authors.affiliation: Microbiology and Environmental Toxicology Department, University of California, Santa Cruz, California 95064, United States.
authors.forename: Rachel R
authors.initials: RR
authors.lastname: Eastman
authors.forename: Tom P
authors.initials: TP
authors.lastname: Jursa
authors.forename: Chiara
authors.initials: C
authors.lastname: Benedetti
authors.forename: Roberto G
authors.initials: RG
authors.lastname: Lucchini
authors.forename: Donald R
authors.initials: DR
authors.lastname: Smith
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D002648:Child; D004784:Environmental Monitoring; D004785:Environmental Pollutants / Q000032:analysis* / Q000302:isolation & purification; D005260:Female; D006197:Hair / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D007558:Italy; D008297:Male; D008345:Manganese / Q000032:analysis* / Q000302:isolation & purification; D008875:Middle Aged; D017942:Nitric Acid / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D013010:Sonication; D014874:Water Pollutants, Chemical / Q000032:analysis; D055815:Young Adult
chemical_list: D015415:Biomarkers; D004785:Environmental Pollutants; D014874:Water Pollutants, Chemical; D011092:Polyethylene Glycols; D017942:Nitric Acid; D008345:Manganese