title: Defatted Jatropha curcas flour and protein isolate as materials for protein hydrolysates with biological activity.
abstract: Jatropha curcas L. protein hydrolysates were produced by treatment of a non-toxic genotype with Alcalase as well as the digestive enzymes pepsin and pancreatin. The J. curcas protein hydrolysate produced with the pepsin-pancreatin system from protein isolate had the highest TEAC value and was shown to undergo single-electron transfer reactions in the ABTS(+) reduction assay, demonstrating its antioxidant capacity. Testing of antimicrobial activity in the J. curcas protein hydrolysates against seven bacterial pathogens showed no growth inhibitory effect in Gram-negative and Gram-positive bacteria. More ACE-I inhibitory active peptides were produced in the Alcalase hydrolysates obtained from J. curcas protein isolate. The protein hydrolysate obtained with Alcalase from defatted J. curcas flour as well as from the protein isolate showed the highest inhibitory effect of ADP-induced aggregation of human platelets in platelet-rich plasma. It is expected that the information collated will facilitate new applications of proteins present in Jatropha plant.
authors.affiliation: Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Periférico Nte. Km. 33.5, Tablaje Catastral 13615, Col. Chuburná de Hidalgo Inn, 97203 Mérida, Yucatán, Mexico.
authors.forename: Duly M
authors.initials: DM
authors.lastname: Marrufo-Estrada
authors.forename: Maira R
authors.initials: MR
authors.lastname: Segura-Campos
authors.forename: Luis A
authors.initials: LA
authors.lastname: Chel-Guerrero
authors.forename: David A
authors.initials: DA
authors.lastname: Betancur-Ancona
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology; D005223:Fats / Q000737:chemistry; D005433:Flour / Q000032:analysis*; D006801:Humans; D031286:Jatropha / Q000737:chemistry*; D007703:Peptidyl-Dipeptidase A / Q000032:analysis; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011492:Protein Hydrolysates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000900:Anti-Bacterial Agents; D005223:Fats; D010940:Plant Proteins; D010975:Platelet Aggregation Inhibitors; D011492:Protein Hydrolysates; D007703:Peptidyl-Dipeptidase A