title: Development of an SPE-HPLC-MS method for simultaneous determination and pharmacokinetic study of bioactive constituents of Yu Ping Feng San in rat plasma after oral administration.
abstract: BACKGROUND
  Yu Ping Feng San (YPFS, in Chinese: Jade Windscreen Powder), a well-known traditional Chinese medicine, is commonly used to cure the diseases of respiratory systems and immune systems.
  
  
  OBJECTIVE
  A selective and sensitive high-performance liquid chromatography coupled with mass spectrometry method (HPLC-MS) was developed and validated for simultaneous quantification of cycloastragenol, formononetin, calycosin, 4'-O-β-glucopyranosyl-5-O-methylvisamminol (GMV) and cimifugin in rat plasma after oral administration of Yu Ping Feng San decoction.
  
  
  METHODS
  Plasma samples were extracted via solid-phase extraction (SPE), separated on a Zorbax SB-C18 column, detected by single quadruple mass spectrometry with an electrospray ionization interface, and quantified using selected ion monitoring mode. The current SPE-HPLC-MS assay was validated for linearity, intra-day and inter-day precisions, accuracy, extraction recovery and stability. The method was applied to a comparative pharmacokinetic study after administration of Yu Ping Feng San to rats at different doses (10, 20 and 40g/kg).
  
  
  RESULTS
  The calibration curves were linear over the ranges 0.50-50ng/mL and 17.36-1736ng/mL. Intra- and inter-day precisions (relative standard deviations) were from 0.45% to 10.95%, and accuracy (relative recovery) from 95% to 115%. The extraction recoveries were greater than 88.42% for all analytes. Dose-dependence was shown for some constituents in the drug concentration-time profiles. Among all the active ingredients detected, cimifugin had the highest blood concentration (881-1510ng/mL), and cycloastragenol had the longest retention time in the rat body (15.06-20.44h).
  
  
  CONCLUSIONS
  This analytical method is a selective, sensitive, precise, accurate, and reliable assay for simultaneous determination of cycloastragenol, calycosin, formononetin, GMV, and cimifugin in rat plasma.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No. 16, Nanxiaojie, Dongzhimennei, Beijing 100700, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Wang
authors.forename: Yanli
authors.initials: Y
authors.lastname: Wang
authors.forename: Rixin
authors.initials: R
authors.lastname: Liang
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.forename: Huihui
authors.initials: H
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Weipeng
authors.initials: W
authors.lastname: Yang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002867:Chromones / Q000097:blood*; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D007529:Isoflavones / Q000097:blood*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012502:Sapogenins / Q000097:blood*; D052616:Solid Phase Extraction / Q000379:methods
chemical_list: D002867:Chromones; D004365:Drugs, Chinese Herbal; D007529:Isoflavones; D012502:Sapogenins; C533198:cimifugin; C506362:yu ping feng san; C121707:7,3'-dihydroxy-4'-methoxyisoflavone; C007768:formononetin; C061014:cycloastragenol