title: Cep164 mediates vesicular docking to the mother centriole during early steps of ciliogenesis.
abstract: Cilia formation is a multi-step process that starts with the docking of a vesicle at the distal part of the mother centriole. This step marks the conversion of the mother centriole into the basal body, from which axonemal microtubules extend to form the ciliary compartment. How vesicles are stably attached to the mother centriole to initiate ciliary membrane biogenesis is unknown. Here, we investigate the molecular role of the mother centriolar component Cep164 in ciliogenesis. We show that Cep164 was indispensable for the docking of vesicles at the mother centriole. Using biochemical and functional assays, we identified the components of the vesicular transport machinery, the GEF Rabin8 and the GTPase Rab8, as interacting partners of Cep164. We propose that Cep164 is targeted to the apical domain of the mother centriole to provide the molecular link between the mother centriole and the membrane biogenesis machinery that initiates cilia formation.
authors.affiliation: DKFZ-ZMBH Alliance, German Cancer Research Center, 69120 Heidelberg, Germany.
authors.forename: Kerstin N
authors.initials: KN
authors.lastname: Schmidt
authors.forename: Stefanie
authors.initials: S
authors.lastname: Kuhns
authors.forename: Annett
authors.initials: A
authors.lastname: Neuner
authors.forename: Birgit
authors.initials: B
authors.lastname: Hub
authors.forename: Hanswalter
authors.initials: H
authors.lastname: Zentgraf
authors.forename: Gislene
authors.initials: G
authors.lastname: Pereira
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000818:Animals; D001324:Autoantigens / Q000378:metabolism; D001665:Binding Sites; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism; D002460:Cell Line; D002502:Centrioles / Q000378:metabolism*; D002923:Cilia / Q000378:metabolism / Q000502:physiology*; D003598:Cytoskeletal Proteins; D015870:Gene Expression; D000081410:Germinal Center Kinases; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008868:Microtubule Proteins / Q000502:physiology*; D008870:Microtubules / Q000378:metabolism; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D022161:Transport Vesicles / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D001324:Autoantigens; C524694:CEP164 protein, human; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D000081410:Germinal Center Kinases; C098623:IFT88 protein, human; D008565:Membrane Proteins; D008868:Microtubule Proteins; C108725:NPHP1 protein, human; C086074:PCM1 protein, human; D025521:Tumor Suppressor Proteins; D017346:Protein Serine-Threonine Kinases