title: Myogenic potential of mesenchymal stem cells - the case of adhesive fraction of human umbilical cord blood cells.
abstract: Different sources of stem cells are considered as a potential source of precursor cells that could improve skeletal muscle regeneration. Under physiological conditions muscle regeneration is based on the satellite cells, i.e. adult muscle precursor cells that are localized between muscle fiber and surrounding basal lamina. These cells remain quiescent but after skeletal muscle injury activate, proliferate, differentiate, and fuse either to form new muscle fibers or reconstruct the damaged ones. As it was shown in many studies few populations of stem cells other than satellite cells are able to support skeletal muscle regeneration. Among them are mesenchymal stem cells (MSCs) that are present in many niches within adult organism and also in fetal tissues, such as human umbilical cord blood (HUCB) or umbilical cord connective tissue, i.e. Wharton's jelly. Thus, MSCs are intensively tested to prove that they are able to differentiate into various cell types, including skeletal myoblasts, and therefore could be useful in regenerative medicine. In our previous study we showed that MSCs isolated from Wharton's jelly expressed pluripotency as well as myogenic markers and were able to undergo myogenic differentiation both in vitro and in vivo. We also analyzed the potential of HUCB cells population which contains not only MSCs but also hematopoietic precursors. Our analyses of whole population of HUCB cells showed that these cells express myogenic regulatory factors, i.e. MyoD, and are able to contribute to skeletal muscle regeneration. In the present study we document that adherent fraction of HUCB cells, i.e. the cells that constitute the subpopulation enriched in MSCs, expresses pluripotency and myogenic markers, and have a positive impact at the regeneration of injured mouse skeletal muscle.
authors.affiliation: Department of Cytology, Institute of Zoology, Faculty of Biology, University of Warsaw, Ilji Miecznikowa 1, 02-096 Warsaw, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Grabowska
authors.forename: Wladyslawa
authors.initials: W
authors.lastname: Streminska
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Janczyk-Ilach
authors.forename: Eugeniusz K
authors.initials: EK
authors.lastname: Machaj
authors.forename: Zygmunt
authors.initials: Z
authors.lastname: Pojda
authors.forename: Grazyna
authors.initials: G
authors.lastname: Hoser
authors.forename: Jerzy
authors.initials: J
authors.lastname: Kawiak
authors.forename: Jerzy
authors.initials: J
authors.lastname: Moraczewski
authors.forename: Maria A
authors.initials: MA
authors.lastname: Ciemerych
authors.forename: Edyta
authors.initials: E
authors.lastname: Brzoska
mesh_terms: D000328:Adult; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D005312:Fetal Blood / Q000166:cytology* / Q000502:physiology; D006801:Humans; D007124:Immunoenzyme Techniques; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D024510:Muscle Development / Q000502:physiology*; D018482:Muscle, Skeletal / Q000166:cytology / Q000502:physiology*; D018006:Myogenic Regulatory Factors / Q000235:genetics / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D044968:Regenerative Medicine; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018006:Myogenic Regulatory Factors; D012333:RNA, Messenger