title: Screening of mutant strain Streptomyces mediolani sp. AC37 for (-)-8-O-methyltetrangomycin production enhancement.
abstract: Streptomyces mediolani sp. AC37 was isolated from the root system of higher plant Taxus baccata and produced metabolite identified as (-)-8-O-methyltetrangomycin according to LC/MS/MS analysis. In our screening program for improvements of bioactive secondary metabolites from plant associate streptomycetes, mutation was used as a tool for the induction of genetic variations for selection of higher (-)-8-O-methyltetrangomycin producers of isolates. S. mediolani sp. AC37 was treated with UV irradiation and chemical mutagenic treatment (N-nitroso-N-methyl-urea). The radical scavenging and antioxidant capacity of (-)-8-O-methyltetrangomycin and extracts isolated from mutants were tested using EPR spin trapping technique and ABTS(·+) assay. Comparison of electron microscopic images of Streptomyces sp. AC37 and mutant strains of Streptomyces sp. AC37 revealed substantial differences in morphology and ultrastructure.
authors.affiliation: Institute of Biotechnology and Food Science, Slovak University of Technology in Bratislava, Radlinského 9, SK-812 37 Bratislava, Slovak Republic. jakeline.trejos.jimenez@gmail.com
authors.forename: Jakeline Trejos
authors.initials: JT
authors.lastname: Jiménez
authors.forename: Maria
authors.initials: M
authors.lastname: Sturdíková
authors.forename: Vlasta
authors.initials: V
authors.lastname: Brezová
authors.forename: Emil
authors.initials: E
authors.lastname: Svajdlenka
authors.forename: Marta
authors.initials: M
authors.lastname: Novotová
mesh_terms: D000880:Anthraquinones / Q000737:chemistry / Q000378:metabolism; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D009154:Mutation; D013302:Streptomyces / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D000095602:Angucyclines and Angucyclinones
chemical_list: D000880:Anthraquinones; D000975:Antioxidants; C059960:MM 47755; D000095602:Angucyclines and Angucyclinones