title: Cytotoxicity and structure activity relationships of phytosterol from Phyllanthus emblica.
abstract: Fourteen sterols (1-14), including two new sterols, trihydroxysitosterol (2) and 5α,6β,7α-7α-acetoxysitosterol (3), were isolated from the branches and leaves of Phyllanthus emblica L. The isolated compounds and a structurally related sterol 15 from Aphanamixis grandifolia were screened for cytotoxicity in two tumor cell lines (HL-60 and SMMC-7721) and a non-tumor cell line (HL-7702) using RSB assay. Within the series of phytosterol derivatives tested, compound 15 was the most active, displaying potent cytotoxicity against HL-60 with IC(50) of 5.10μmol/L, and most of the active compounds showed selective cytotoxicity against tumor and non-tumor cell lines, especially compound 10 with a safety index of 4.42.
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, People's Republic of China.
authors.forename: Wei-Yan
authors.initials: WY
authors.lastname: Qi
authors.forename: Ya
authors.initials: Y
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Hua
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.forename: Kun
authors.initials: K
authors.lastname: Gao
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy; D002460:Cell Line; D006801:Humans; D007938:Leukemia / Q000188:drug therapy; D008113:Liver Neoplasms / Q000188:drug therapy; D015394:Molecular Structure; D029842:Phyllanthus emblica / Q000737:chemistry*; D010840:Phytosterols / Q000737:chemistry* / Q000494:pharmacology*; D035261:Plant Components, Aerial / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010840:Phytosterols