title: Distinct interactions of Na+ and Ca2+ ions with the selectivity filter of the bacterial sodium channel Na(V)Ab.
abstract: Rapid and selective ion transport is essential for the generation and regulation of electrical signaling pathways in living organisms. In this study, we use molecular dynamics simulations and free energy calculations to investigate how the bacterial sodium channel Na(V)Ab (Arcobacter butzleri) differentiates between Na(+) and Ca(2+) ions. Multiple nanosecond molecular dynamics simulations revealed distinct binding patterns for these two cations in the selectivity filter and suggested a high affinity calcium binding site formed by backbone atoms of residues Leu-176 and Thr-175 (S(CEN)) in the sodium channel selectivity filter.
authors.affiliation: Department of Pharmacology and Toxicology, University of Vienna, Althanstrasse 14, UZA 2, A-1090 Vienna, Austria. song.ke@univie.ac.at
authors.forename: Song
authors.initials: S
authors.lastname: Ke
authors.forename: Eva-Maria
authors.initials: EM
authors.lastname: Zangerl
authors.forename: Anna
authors.initials: A
authors.lastname: Stary-Weinzinger
mesh_terms: D020640:Arcobacter / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002413:Cations, Divalent / Q000378:metabolism; D002414:Cations, Monovalent / Q000378:metabolism; D056004:Molecular Dynamics Simulation; D012964:Sodium / Q000378:metabolism*; D015222:Sodium Channels / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002413:Cations, Divalent; D002414:Cations, Monovalent; D015222:Sodium Channels; D012964:Sodium; D002118:Calcium