title: Leukemogenesis as a new approach to investigate the correlation between up regulated gene 4/upregulator of cell proliferation (URG4/URGCP) and signal transduction genes in leukemia.
abstract: The aim of the study is to the determine the profiles of cell cycle genes and a new candidate oncogene of URG4/URGCP which play role in leukemia, establishing the association between the early prognosis of cancer and the quantitation of genetic changes, and bringing a molecular approach to definite diagnosis. In this study, 36 newly diagnosed patients' with ALL-AML in the range of 0-18 years and six control group patients' bone marrow samples were included. Total RNA was isolated from samples and then complementary DNA synthesis was performed. The obtained cDNAs have been installed 96 well plates after prepared appropriate mixtures and assessed with LightCycler(®) 480 Real-Time PCR quantitatively. CHEK1, URG4/URGCP, CCNG1, CCNC, CDC16, KRAS, CDKN2D genes in the T-ALL group; CCND2, ATM, CDK8, CHEK1, TP53, CHEK2, CCNG2, CDK4, CDKN2A, E2F4, CCNC, KRAS genes in the precursor B-ALL group and CCND2, CDK6 genes in the AML group have shown significant increase in mRNA expression level. In the featured role of acute leukemia the regulating signaling pathways of leukemogenesis partially defined, although identification of new genetic markers in acute leukemia subgroups, will allow the development of early diagnostic and new treatment protocols.
authors.affiliation: Department of Medical Biology, School of Medicine, Pamukkale University, Kınıklı Kampüsü Morfoloji Binasi Kat:3, Kınıklı/Denizli, Turkey. yavuzdodurga@gmail.com
authors.forename: Yavuz
authors.initials: Y
authors.lastname: Dodurga
authors.forename: Yeşim
authors.initials: Y
authors.lastname: Oymak
authors.forename: Cumhur
authors.initials: C
authors.lastname: Gündüz
authors.forename: N Lale
authors.initials: NL
authors.lastname: Satıroglu-Tufan
authors.forename: Canan
authors.initials: C
authors.lastname: Vergin
authors.forename: Nazan
authors.initials: N
authors.lastname: Cetingül
authors.forename: Cığır
authors.initials: C
authors.lastname: Biray Avci
authors.forename: Nejat
authors.initials: N
authors.lastname: Topçuoğlu
mesh_terms: D000293:Adolescent; D059447:Cell Cycle Checkpoints / Q000235:genetics*; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic; D002648:Child; D002675:Child, Preschool; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000473:pathology; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics*; D011379:Prognosis; D015398:Signal Transduction; D015854:Up-Regulation
chemical_list: D009363:Neoplasm Proteins; C470990:URGCP protein, human