title: [Effect of polysaccharides from Radix Glycyrrhizae on migration and polyamines contents of IEC-6 cell].
abstract: OBJECTIVE
  To study the effect of polysaccharides from Radix Glycyrrhizae on migration and polyamines (putrescine, spermidine and spermine) contents of IEC-6 cell.
  
  
  METHODS
  Cell migration model was induced by scratch method in each well,and the polyamines in IEC-6 cell was determined by pre-column derivation high performance liquid chromatography. The polysaccharides inhibited effect on migration and polyamines contents of IEC-6 cells, and on IEC-6 cell migration by DFMO (a polyamines synthesis inhibitor) and the polyamines contents in the cells were observed.
  
  
  RESULTS
  The polysaccharides (50 mg/L or 100 mg/L) was able to promote the cell migration, reverse the cell migration inhibition by DFMO, enhance the IEC-6 cell polyamines (putrescine, spermidine and spermine) contents in the process of cell migration and reverse the reduction of polyamines (putrescine, spermidine and spermine) induced by DFMO.
  
  
  CONCLUSIONS
  The effect of Radix Glycyrrhizae on the gastrointestinal mucosal damage repairing may be related to increasing polyamine content in cells and promoting cell migration.
authors.affiliation: Piwei Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, China. wenpengsweet@163.com
authors.forename: Peng
authors.initials: P
authors.lastname: Wen
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Sui
authors.forename: Ru-Liu
authors.initials: RL
authors.lastname: Li
authors.forename: Shi-Qing
authors.initials: SQ
authors.lastname: Zhao
authors.forename: Wen-Biao
authors.initials: WB
authors.lastname: Lu
authors.forename: Wei-Wen
authors.initials: WW
authors.lastname: Chen
mesh_terms: D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D000518:Eflornithine / Q000037:antagonists & inhibitors; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006035:Glycyrrhiza / Q000737:chemistry*; D007413:Intestinal Mucosa / Q000166:cytology* / Q000378:metabolism / Q000473:pathology; D018517:Plant Roots / Q000737:chemistry; D011073:Polyamines / Q000378:metabolism*; D011134:Polysaccharides / Q000494:pharmacology*; D051381:Rats; D027343:Rhizome / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D011073:Polyamines; D011134:Polysaccharides; D000518:Eflornithine