title: Isolation and characterization of a low pathogenic duck hepatitis A virus 3 from South Korea.
abstract: The D11-JW-018 strain of duck hepatitis A virus (DHAV) was isolated from 7-day-old ducklings in Kyeonggi province, South Korea with no clinical signs of typical hepatitis. Phylogenetic analyzed of whole genome showed that D11-JW-018 strain was belonged to DHAV-3 genotype. The pathogenicity of the D11-JW-018 strain in 1-, 7-, 14-, and 21-day-old ducklings was examined. Mortality of D11-JW-018 strain was lower than DRL-62 (DHAV-1) age-dependent but incubation period was longer in 1-day-old ducklings. Unlike DRL-62 strain infection, D11-JW-018 strain induced only liver discoloration without hemorrhagic mottling and lymphocyte infiltration and bile duct hyperplasia in histological lesion. The D11-JW-018 strain was detected only in the heart, liver, spleen, gall bladder, pancreas, and kidney among 12 organs in infected 1-day-old ducklings. Serum biochemical analyses revealed a significant difference in aspartate transaminase and alanine transaminase between the D11-JW-018 strain-infected ducklings and those infected with the DRL-62 strain (P<0.05). We identified the D11-JW-018 strain in South Korean ducklings and provide the characteristics of DHAV-3.
authors.affiliation: Department of Infectious Diseases & Avian Diseases, College of Veterinary Medicine and Research, Division of Influenza Korea Zoonosis Research Institute, Chonbuk National University, Jeonju 561-756, South Korea.
authors.forename: Se-Yeoun
authors.initials: SY
authors.lastname: Cha
authors.forename: Jae-Hee
authors.initials: JH
authors.lastname: Roh
authors.forename: Min
authors.initials: M
authors.lastname: Kang
authors.forename: Bumseok
authors.initials: B
authors.lastname: Kim
authors.forename: Hyung-Kwan
authors.initials: HK
authors.lastname: Jang
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D004372:Ducks; D004371:Hepatitis Virus, Duck / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006524:Hepatitis, Viral, Animal / Q000201:enzymology / Q000473:pathology / Q000821:virology*; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000201:enzymology / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D011201:Poultry Diseases / Q000201:enzymology / Q000473:pathology / Q000821:virology*; D056910:Republic of Korea
chemical_list: D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase