title: Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR.
abstract: We validated the use of stored samples for Chlamydia trachomatis research. C. trachomatis DNA was detected by real-time PCR in clinical (urine and self-taken vaginal swabs) and spiked samples using six different media, five different time points (up to 2 years), and four different temperature conditions. C. trachomatis was detected in all 423 samples, and no clinically relevant degradation impact was detected.
authors.affiliation: Department of Medical Microbiology, Maastricht University Medical Centre, Maastricht, The Netherlands.
authors.forename: Laura
authors.initials: L
authors.lastname: van Dommelen
authors.forename: Petra F G
authors.initials: PF
authors.lastname: Wolffs
authors.forename: Frank H
authors.initials: FH
authors.lastname: van Tiel
authors.forename: Nicole
authors.initials: N
authors.lastname: Dukers
authors.forename: Selma B
authors.initials: SB
authors.lastname: Herngreen
authors.forename: Cathrien A
authors.initials: CA
authors.lastname: Bruggeman
authors.forename: Christian J P A
authors.initials: CJ
authors.lastname: Hoebe
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D002690:Chlamydia Infections / Q000175:diagnosis; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification*; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D013048:Specimen Handling / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D014556:Urine / Q000382:microbiology; D014621:Vagina / Q000821:virology
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial