title: High-level production of thermotolerant β-xylosidase of Aspergillus sp. BCC125 in Pichia pastoris: characterization and its application in ethanol production.
abstract: A gene coding for thermotolerant β-xylosidase from Aspergillus sp. BCC125 was characterized. The recombinant enzyme was expressed in methylotrophic yeast Pichia pastoris KM71 and especially high yield of secreted enzyme was obtained. β-xylosidase possessed high enzyme efficiency (Kcat/Km=198.8mM(-1)s(-1)) toward pNP-β-D-xylopyranoside (pNPβX) with optimal temperature and pH for activity of 60°C and pH 4.0-5.0, respectively. The identified β-xylosidase showed clear synergism with previously identified xylanase for hydrolysis of xylan in vitro as well as simultaneous saccharification and fermentation process (SSF) in vivo with Pichia stipitis.
authors.affiliation: Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand. sriwan@biotec.or.th
authors.forename: Sriwan
authors.initials: S
authors.lastname: Wongwisansri
authors.forename: Peerada
authors.initials: P
authors.lastname: Promdonkoy
authors.forename: Panida
authors.initials: P
authors.lastname: Matetaviparee
authors.forename: Niran
authors.initials: N
authors.lastname: Roongsawang
authors.forename: Lily
authors.initials: L
authors.lastname: Eurwilaichitr
authors.forename: Sutipa
authors.initials: S
authors.lastname: Tanapongpipat
mesh_terms: D001230:Aspergillus / Q000201:enzymology* / Q000235:genetics; D056804:Biofuels; D001709:Biotechnology / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D000431:Ethanol; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D010843:Pichia / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature; D014990:Xylans / Q000378:metabolism*; D014995:Xylosidases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D056804:Biofuels; D017931:DNA Primers; D014990:Xylans; D000431:Ethanol; D014995:Xylosidases; C026579:exo-1,4-beta-D-xylosidase