title: DNA binding, antioxidant, cytotoxicity (MTT, lactate dehydrogenase, NO), and cellular uptake studies of structurally different nickel(II) thiosemicarbazone complexes: synthesis, spectroscopy, electrochemistry, and X-ray crystallography.
abstract: Three new nickel(II) thiosemicarbazone complexes have been synthesized and characterized by analytical, spectral, and single-crystal X-ray diffraction studies. In complex 1, the ligand 2-hydroxy-1-naphthaldehydethiosemicarbazone coordinated as a monobasic tridentate donor, whereas in complexes 2 and 3, the ligands salicylaldehyde-4(N)-ethylthiosemicarbazone and 2-hydroxy-1-naphthaldehyde-4(N)-ethylthiosemicarbazone coordinated as a dibasic tridentate donor. The DNA binding ability of the complexes in calf thymus DNA was explored by absorption and emission titration experiments. The antioxidant property of the new complexes was evaluated to test their free-radical scavenging ability. In vitro cytotoxicity assays were performed for the new complexes in A549 and HepG2 cell lines. The new compounds overcome cisplatin resistance in the A549 cell line and they were also active in the HepG2 cell line. The cellular uptake study showed the accumulation of the complexes in tumor cells depended on the nature of the ligand attached to the nickel ion.
authors.affiliation: Department of Chemistry, Bharathiar University, Coimbatore, 641046, India. rpnchemist@gmail.com.
authors.forename: R
authors.initials: R
authors.lastname: Prabhakaran
authors.affiliation: Department of Chemistry, Bharathiar University, Coimbatore, 641046, India.
authors.forename: P
authors.initials: P
authors.lastname: Kalaivani
authors.affiliation: Department of Chemistry, Michigan State University, East Lansing, MI, 48824, USA.
authors.forename: R
authors.initials: R
authors.lastname: Huang
authors.affiliation: Department of Biotechnology, Bharathiar University, Coimbatore, 641046, India.
authors.forename: P
authors.initials: P
authors.lastname: Poornima
authors.affiliation: Department of Biotechnology, Bharathiar University, Coimbatore, 641046, India.
authors.forename: V
authors.initials: V
authors.lastname: Vijaya Padma
authors.affiliation: Laboratoire Chimie Provence-CNRS UMR6264, Université of Aix-Marseille I, II and III-CNRS, Campus Scientifique de Saint-Jérôme, Avenue Escadrille Normandie-Niemen, 13397, Marseille Cedex 20, France.
authors.forename: F
authors.initials: F
authors.lastname: Dallemer
authors.affiliation: Department of Chemistry, Bharathiar University, Coimbatore, 641046, India. k_natraj6@yahoo.com.
authors.forename: K
authors.initials: K
authors.lastname: Natarajan
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D056831:Coordination Complexes / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D004563:Electrochemistry; D005562:Formazans / Q000737:chemistry / Q000378:metabolism; D016166:Free Radical Scavengers / Q000737:chemistry; D005609:Free Radicals / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D006860:Hydrogen Bonding; D020128:Inhibitory Concentration 50; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008958:Models, Molecular; D008968:Molecular Conformation; D009532:Nickel / Q000737:chemistry*; D009569:Nitric Oxide / Q000378:metabolism; D010084:Oxidation-Reduction; D010851:Picrates / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D013778:Tetrazolium Salts / Q000737:chemistry / Q000378:metabolism; D013882:Thiosemicarbazones / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D001713:Biphenyl Compounds; D056831:Coordination Complexes; D005562:Formazans; D016166:Free Radical Scavengers; D005609:Free Radicals; D010851:Picrates; D013778:Tetrazolium Salts; D013882:Thiosemicarbazones; C079782:MTT formazan; D009569:Nitric Oxide; C022838:nickel chloride; D009532:Nickel; D004247:DNA; C427203:calf thymus DNA; C004931:1,1-diphenyl-2-picrylhydrazyl; D007770:L-Lactate Dehydrogenase