title: TRICOT encodes an AMP1-related carboxypeptidase that regulates root nodule development and shoot apical meristem maintenance in Lotus japonicus.
abstract: During the course of evolution, mainly leguminous plants have acquired the ability to form de novo structures called root nodules. Recent studies on the autoregulation and hormonal controls of nodulation have identified key mechanisms and also indicated a possible link to other developmental processes, such as the formation of the shoot apical meristem (SAM). However, our understanding of nodulation is still limited by the low number of nodulation-related genes that have been identified. Here, we show that the induced mutation tricot (tco) can suppress the activity of spontaneous nodule formation 2, a gain-of-function mutation of the cytokinin receptor in Lotus japonicus. Our analyses of tco mutant plants demonstrate that TCO positively regulates rhizobial infection and nodule organogenesis. Defects in auxin regulation are also observed during nodule development in tco mutants. In addition to its role in nodulation, TCO is involved in the maintenance of the SAM. The TCO gene was isolated by a map-based cloning approach and found to encode a putative glutamate carboxypeptidase with greatest similarity to Arabidopsis ALTERED MERISTEM PROGRAM 1, which is involved in cell proliferation in the SAM. Taken together, our analyses have not only identified a novel gene for regulation of nodule organogenesis but also provide significant additional evidence for a common genetic regulatory mechanism in nodulation and SAM formation. These new data will contribute further to our understanding of the evolution and genetic basis of nodulation.
authors.affiliation: Division of Symbiotic Systems, National Institute for Basic Biology, Okazaki, Aichi 444-8585, Japan. tsuzaki@nibb.ac.jp
authors.forename: Takuya
authors.initials: T
authors.lastname: Suzaki
authors.forename: Chong Sung
authors.initials: CS
authors.lastname: Kim
authors.forename: Naoya
authors.initials: N
authors.lastname: Takeda
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Szczyglowski
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Kawaguchi
mesh_terms: D017360:Arabidopsis / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002268:Carboxypeptidases / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D003583:Cytokinins / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005838:Genotype; D017403:In Situ Hybridization; D000070116:Lotus / Q000201:enzymology* / Q000502:physiology*; D018519:Meristem / Q000502:physiology*; D008957:Models, Genetic; D009154:Mutation; D010641:Phenotype; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D003583:Cytokinins; D010940:Plant Proteins; D002268:Carboxypeptidases; C578394:TRICOT protein, Lotus japonicus; C506681:AMP1 protein, Arabidopsis