title: Semi-automated method for estimating lesion volumes.
abstract: Accurately measuring the volume of tissue damage in experimental lesion models is crucial to adequately control for the extent and location of the lesion, variables that can dramatically bias the outcome of preclinical studies. Many of the current commonly used techniques for this assessment, such as measuring the lesion volume with primitive software macros and plotting the lesion location manually using atlases, are time-consuming and offer limited precision. Here we present an easy to use semi-automated computational method for determining lesion volume and location, designed to increase precision and reduce the manual labor required. We compared this novel method to currently used methods and demonstrate that this tool is comparable or superior to current techniques in terms of precision and has distinct advantages with respect to user interface, labor intensiveness and quality of data presentation.
authors.affiliation: Department of Neurosciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
authors.forename: Hyun-Joo
authors.initials: HJ
authors.lastname: Park
authors.forename: Andre G
authors.initials: AG
authors.lastname: Machado
authors.forename: Jessica
authors.initials: J
authors.lastname: Cooperrider
authors.forename: Havan
authors.initials: H
authors.lastname: Truong-Furmaga
authors.forename: Matthew
authors.initials: M
authors.lastname: Johnson
authors.forename: Vibhuti
authors.initials: V
authors.lastname: Krishna
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Chen
authors.forename: John T
authors.initials: JT
authors.lastname: Gale
mesh_terms: D000465:Algorithms; D019412:Anatomy, Cross-Sectional; D000818:Animals; D001331:Automation; D001921:Brain / Q000473:pathology*; D016232:Endothelins / Q000008:administration & dosage / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted; D051381:Rats; D012984:Software; D013238:Stereotaxic Techniques; D020521:Stroke / Q000139:chemically induced / Q000473:pathology; D016707:Tissue Fixation
chemical_list: D016232:Endothelins