title: Histopathological diagnosis of early HCC through biopsy: efficacy of Victoria blue and cytokeratin 7 staining.
abstract: OBJECTIVE
  Findings of histological analyses of 2 cases of liver biopsy revealing hypovascular nodules are described.
  
  
  RESULTS
  Ultrasound examination revealed hypovascular and hypoechoic nodules (8 mm in diameter) in segment 1 (case 1) and (8 mm) in segment 8 (case 2). The nodules were detected by only Gd-EOB-DTPA-enhanced MRI. Hematoxylin and eosin staining of ultrasound-guided biopsy of the nodules revealed slight hypercellularity without the features of early hepatocellular carcinoma (HCC) such as cell atypia, fatty change and pseudoglandular formation. Early HCC was suspected; however, Victoria blue staining disclosed terminal portal tract invasion, the most important finding of early HCC. Also, cytokeratin 7 staining revealed decreased ductular reaction compatible with early HCC. Taken together, these histological analyses confirmed the two nodules to be early HCC.
  
  
  CONCLUSIONS
  Based on the criteria of the International Consensus Group, the two nodules were diagnosed as early HCC through biopsy.
authors.affiliation: Department of Hepatology, Osaka City University Graduate School of Medicine, Osaka, Japan.
authors.forename: Sawako
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Soo Ryang
authors.initials: SR
authors.lastname: Kim
authors.forename: Susumu
authors.initials: S
authors.lastname: Imoto
authors.forename: Kenji
authors.initials: K
authors.lastname: Ando
authors.forename: Makoto
authors.initials: M
authors.lastname: Hirakawa
authors.forename: Jun
authors.initials: J
authors.lastname: Saito
authors.forename: Katsumi
authors.initials: K
authors.lastname: Fukuda
authors.forename: Yumi
authors.initials: Y
authors.lastname: Otono
authors.forename: Madoka
authors.initials: M
authors.lastname: Sakaki
authors.forename: Shinobu
authors.initials: S
authors.lastname: Tsuchida
authors.forename: Soo Ki
authors.initials: SK
authors.lastname: Kim
authors.forename: Yoshitake
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Nakano
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Kudo
mesh_terms: D000369:Aged, 80 and over; D001706:Biopsy; D006528:Carcinoma, Hepatocellular / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D004396:Coloring Agents; D055088:Early Detection of Cancer; D006801:Humans; D007150:Immunohistochemistry; D053552:Keratin-7 / Q000378:metabolism*; D008099:Liver / Q000473:pathology*; D008113:Liver Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009930:Organic Chemicals; D014463:Ultrasonography
chemical_list: D004396:Coloring Agents; D053552:Keratin-7; D009930:Organic Chemicals; C041370:victoria blue