title: A monoclonal antibody against bovine adiponectin.
abstract: Adiponectin (AdipoQ) is an adipokine mainly secreted by white fatty tissue, playing a major role in energy homeostasis and insulin sensitivity. For cattle, AdipoQ data are largely limited to mRNA expression; to our knowledge, valid information about the AdipoQ protein in bovine tissues and body fluids is not available. Therefore, we have developed a monoclonal antibody against bovine AdipoQ. This study describes the preparation, application, and characterization of a monoclonal antibody for use in ELISA, Western blot, and histology. The antibody was developed by PEG fusion of the SP2/0 cell line with splenic B cells from AdipoQ immunized C57Bl/6 mice. Antibody-producing cells were identified by ELISA and specified by immunoblotting and immunostaining of bovine retroperitoneal adipose tissue. The novel antibody detects AdipoQ in histological samples, ELISA, and Western blots.
authors.affiliation: University of Applied Sciences Bonn-Rhein-Sieg, Immunology and Cell Biology, Rheinbach, Germany .
authors.forename: Michael
authors.initials: M
authors.lastname: Raffelsieper
authors.forename: Birgit
authors.initials: B
authors.lastname: Mielenz
authors.forename: Susanne
authors.initials: S
authors.lastname: Häußler
authors.forename: Helga
authors.initials: H
authors.lastname: Sauerwein
authors.forename: Manfred
authors.initials: M
authors.lastname: Mielenz
authors.forename: Harald
authors.initials: H
authors.lastname: Illges
mesh_terms: D052242:Adiponectin / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000096:biosynthesis / Q000737:chemistry* / Q000276:immunology; D000918:Antibody Specificity; D015153:Blotting, Western; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas; D007150:Immunohistochemistry; D050152:Intra-Abdominal Fat / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D052242:Adiponectin; D058846:Antibodies, Monoclonal, Murine-Derived