title: [Induction of human dendritic cells from peripheral blood mononuclear cells by using a HydroCellTM].
abstract: Cancer immunotherapy using dendritic cells (DCs) is widely known to produce an anticancer effects. However, there is no approved DC vaccine in Japan, possibly because the culture and harvesting of DC is complicated. To further enable DC vaccine therapy, an easier, safer, and inexpensive culture system is needed, and we therefore aimed to build an automated DC culture system. In a previous study using a HydroCellTM, we successfully cultured and harvested DCs from peripheral blood monocytes without using enzymes, and showed that HydroCellTM is a useful tool for automated DC culture. In this study, we performed a similar process of DC culture from peripheral blood mononuclear cells and demonstrated the safe and easy culturing and harvesting of DCs. Furthermore, CD83-positive mature DCs were increased compared to the usual plate method. These results demonstrate the utility of HydroCellTM as an automated DC culture system.
authors.affiliation: Dept. of Gastroenterological Surgery, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Mayuko
authors.initials: M
authors.lastname: Sakai
authors.forename: Atsushi
authors.initials: A
authors.lastname: Aruga
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Kotera
authors.forename: Masakazu
authors.initials: M
authors.lastname: Yamamoto
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*