title: Assessment of human papilloma virus infection in adult laryngeal papilloma using a screening test.
abstract: OBJECTIVE
  Human papilloma virus (HPV) infection is involved in both juvenile and adult laryngeal papilloma. We wished to determine which types of adult laryngeal papilloma were clinically related to HPV infection. We hypothesized that multiple-site and recurrent papillomas would have a strong relationship to HPV and conducted the present study to test this hypothesis.
  
  
  METHODS
  Thirteen male patients with adult laryngeal papilloma who underwent resection of papilloma between August 2006 and September 2009 were studied. We examined the relationships between whether the tumor was solitary or multiple, presence or absence of recurrence after surgery, and HPV infection. High-risk HPV types (HPV-DNA types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 68) and low-risk HPV types (6, 11, 42, 43, and 44) were tested by a liquid-phase hybridization method. In addition, HPV typing was performed for patients positive for low-risk HPV types. Twenty patients with laryngeal carcinoma or laryngeal leukoplakia were enrolled as the control group.
  
  
  RESULTS
  In the laryngeal papilloma group, all patients tested were negative for high-risk HPV and 69.2% were positive for low-risk HPV. Typing performed for seven of the patients who tested positive for low-risk HPV showed that one patient was positive for HPV-11, whereas the remaining six patients were positive for HPV-6. All patients with recurrent laryngeal papillomatosis (RLP) were positive for low-risk HPV. All patients who were positive for low-risk HPV had RLP. Tumor samples from repeat operations were positive for low-risk HPV in all patients tested. HPV was not detected in the control group.
  
  
  CONCLUSIONS
  The relationship between RLP and low-risk HPV was strong, with all cases that were positive for low-risk HPV showing recurrence. Tumor tissue resected at the time of repeat surgery was positive for low-risk HPV in all cases tested.
authors.affiliation: Department of Otorhinolaryngology, Nihon University Surugadai Hospital, Chiyoda, Tokyo 101-8309, Japan. mak@med.email.ne.jp
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Makiyama
authors.forename: Ryoji
authors.initials: R
authors.lastname: Hirai
authors.forename: Hiroumi
authors.initials: H
authors.lastname: Matsuzaki
authors.forename: Minoru
authors.initials: M
authors.lastname: Ikeda
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000302:isolation & purification*; D061809:Human Papillomavirus DNA Tests; D006801:Humans; D007822:Laryngeal Neoplasms / Q000175:diagnosis / Q000601:surgery / Q000821:virology*; D053685:Laser Therapy; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D009378:Neoplasms, Multiple Primary; D010212:Papilloma / Q000175:diagnosis / Q000601:surgery / Q000821:virology*; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000150:complications / Q000175:diagnosis* / Q000821:virology; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D016896:Treatment Outcome
chemical_list: D015344:DNA Probes, HPV; D004279:DNA, Viral