title: A versatile genome-scale PCR-based pipeline for high-definition DNA FISH.
abstract: We developed a cost-effective genome-scale PCR-based method for high-definition DNA FISH (HD-FISH). We visualized gene loci with diffraction-limited resolution, chromosomes as spot clusters and single genes together with transcripts by combining HD-FISH with single-molecule RNA FISH. We provide a database of over 4.3 million primer pairs targeting the human and mouse genomes that is readily usable for rapid and flexible generation of probes.
authors.affiliation: Departments of Physics and Biology, and the Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Magda
authors.initials: M
authors.lastname: Bienko
authors.affiliation: Departments of Physics and Biology, and the Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Nicola
authors.initials: N
authors.lastname: Crosetto
authors.affiliation: Departments of Physics and Biology, and the Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Leonid
authors.initials: L
authors.lastname: Teytelman
authors.affiliation: Departments of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Sandy
authors.initials: S
authors.lastname: Klemm
authors.affiliation: Departments of Physics and Biology, and the Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Shalev
authors.initials: S
authors.lastname: Itzkovitz
authors.affiliation: Departments of Physics and Biology, and the Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.|Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences) and University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, Netherlandss.
authors.forename: Alexander
authors.initials: A
authors.lastname: van Oudenaarden
mesh_terms: D000818:Animals; D017931:DNA Primers; D015698:Genomic Library; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D051379:Mice; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D017931:DNA Primers