title: Ght2⁺ is required for UDP-galactose synthesis from extracellular galactose by Schizosaccharomyces pombe.
abstract: Schizosaccharomyces pombe has eight hexose transporter genes, ght1 (+) to ght8 (+). Here we report that ght2 (+), which is highly expressed in the presence of glucose, is essential for UDP-galactose synthesis from extracellular galactose when cells grow on glucose. The galactosylation defect of a uge1Δ mutant defective in synthesis of UDP-galactose from glucose was suppressed in galactose-containing medium, but disruption of ght2 (+) in the uge1Δ mutant reversed suppression of the galactosylation defect. Expression of Saccharomyces cerevisiae GAL2 in uge1Δght2Δ cells suppressed the defective galactosylation phenotype in galactose-containing medium. These results indicate that galactose is transported from the medium to the cytosol in a Ght2-dependent manner, and is then converted into UDP-galactose.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Matsuzawa
authors.forename: Futoshi
authors.initials: F
authors.lastname: Hara
authors.forename: Naotaka
authors.initials: N
authors.lastname: Tanaka
authors.forename: Hideki
authors.initials: H
authors.lastname: Tohda
authors.forename: Kaoru
authors.initials: K
authors.lastname: Takegawa
mesh_terms: D003470:Culture Media / Q000737:chemistry; D005690:Galactose / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014531:Uridine Diphosphate Galactose / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D009004:Monosaccharide Transport Proteins; D014531:Uridine Diphosphate Galactose; D005947:Glucose; D005690:Galactose