title: Three sulphated polysaccharides isolated from the mucilage of mud snail, Bullacta exarata philippi: characterization and antitumour activity.
abstract: Three sulphated polysaccharides, coded as BEMPA, BEMPB(1), BEMPB(2), were extracted from the mucilage of mud snail of Bullacta exarata and purified by DEAE-cellulose ion-exchange and size-exclusion chromatography. Structural analysis of purified polysaccharides by chemical and biochemical methods revealed BEMPA was a high (1→3,4)-linked mannose-containing polysaccharide with molecular weight of 22,977 Da. BEMPB(1), with molecular weight of 64,117 Da, was a high (1→3)-linked arabinose-containing polysaccharide. BEMPB(2) was mainly composed of (1→3,4)-linked mannose with molecular weight of 47,507Da. The comparison between sulphated polysaccharides and their desulphated products showed that sulphate substitutions of BEMPB(1) were deduced to be at the C-3 of (1→4)-linked mannose, while sulphate substitutions of BEMPA and BEMPB(2) were at C-4 of (1→3)-linked mannose. Furthermore, BEMPA exhibited highest inhibitory effects on growth of B-16 melanoma cells, and IC(50) were 31.1 μg/mL.
authors.affiliation: Department of Food Science and Technology, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Daihui
authors.initials: D
authors.lastname: Zhang
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.forename: Hongxi
authors.initials: H
authors.lastname: Wu
authors.forename: Jingli
authors.initials: J
authors.lastname: Xie
authors.forename: Lei
authors.initials: L
authors.lastname: Du
authors.forename: Zhaoming
authors.initials: Z
authors.lastname: Xia
authors.forename: Jingbo
authors.initials: J
authors.lastname: Cai
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Huang
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Wei
mesh_terms: D000818:Animals; D015394:Molecular Structure; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D012908:Snails / Q000737:chemistry*; D013431:Sulfates
chemical_list: D011134:Polysaccharides; D013431:Sulfates