title: Silencing of the ARP2/3 complex disturbs pancreatic cancer cell migration.
abstract: BACKGROUND
  Actin-related protein 2/3 (ARP2/3) complex is an actin nucleator responsible for actin cytoskeleton branching which is essential for efficient cell migration.
  
  
  METHODS
  The expression of the seven ARP2/3 complex subunits was assessed in pancreatic cancer cell lines and in normal pancreatic samples by quantitative RT-PCR. siRNA-mediated silencing was used to study the contribution of each ARP2/3 complex member to pancreatic cancer cell migration.
  
  
  RESULTS
  ARPC3 and ARPC4 were the most highly expressed complex members, while ARPC1B and ARPC2 were expressed at low levels. Silencing of the ARP2/3 complex subunits typically resulted in reduced cell migration capacity. In particular, silencing of ARPC4 significantly reduced cell migration in all studied cell lines, with a major impact on Hs700T and HPAFII migration (50% and 68% decrease, p<0.001).
  
  
  CONCLUSIONS
  We offer comprehensive expression data on the ARP2/3 complex members for pancreatic cancer and normal pancreas. In addition, we show cell line-specific differences in ARP2/3 complex subunit dependency on cell migratory potential, and suggest ARPC4 to be one of the key members of the ARP2/3 complex in pancreatic cancer.
authors.affiliation: Institute of Biomedical Technology, FIN_33014 University of Tampere, Finland.
authors.forename: Hanna E
authors.initials: HE
authors.lastname: Rauhala
authors.forename: Susanna
authors.initials: S
authors.lastname: Teppo
authors.forename: Sanna
authors.initials: S
authors.lastname: Niemelä
authors.forename: Anne
authors.initials: A
authors.lastname: Kallioniemi
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D051376:Actin-Related Protein 2-3 Complex / Q000235:genetics / Q000378:metabolism; D000199:Actins / Q000235:genetics* / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D034741:RNA, Small Interfering
chemical_list: C581326:ARPC4 protein, human; D051376:Actin-Related Protein 2-3 Complex; D000199:Actins; D003598:Cytoskeletal Proteins; D034741:RNA, Small Interfering