title: Study of SOD mimic and nucleic acid interaction activity exerted by enrofloxacin-based copper(II) complexes.
abstract: Five new copper(II) complexes of type [Cu(erx)(L)Cl] (erx, enrofloxacin; thiophene-2-carbaldehyde (L(1) ); pyridine-2-carbaldehyde (L(2) ); 2,2'-dipyridylamine (L(3) ); 4,5-diazafluoren-9-one (L(4) ); bis(3,5-dimethyl-1-pyrazolyl)methane (L(5) )) have been synthesized and characterized by elemental analysis, reflectance, IR, and FAB-MS. Complexes have been investigated for their interaction with calf thymus (CT) DNA utilizing the absorption-titration method, viscometric and DNA thermal denaturation studies. The cleavage reaction on pUC19 DNA has been monitored by agarose gel electrophoresis. The results indicated that the Cu(II) complexes can more effectively promote the cleavage of plasmid DNA at physiological pH and superoxide dismutase. The (SOD) activity of the complexes has been evaluated by the nitroblue tetrazolium assay, and the complexes catalyzed the dismutation of superoxide at pH 7.8 with IC(50) values of 0.35-1.25 μM. The complexes have also been screened for their antibacterial activity against five pathogenic bacteria.
authors.affiliation: Department of Chemistry, Sardar Patel University, Vallabh Vidyanagar - 388 120, Gujarat, India. jeenen@gmail.com
authors.forename: Mohan N
authors.initials: MN
authors.lastname: Patel
authors.forename: Bhupesh S
authors.initials: BS
authors.lastname: Bhatt
authors.forename: Promise A
authors.initials: PA
authors.lastname: Dosi
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D040761:Biomimetic Materials / Q000737:chemistry / Q000378:metabolism*; D020134:Catalytic Domain; D002417:Cattle; D056831:Coordination Complexes / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000378:metabolism*; D053837:DNA Cleavage / Q000187:drug effects; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000737:chemistry / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D056831:Coordination Complexes; D024841:Fluoroquinolones; D013481:Superoxides; D000077422:Enrofloxacin; D003300:Copper; D004247:DNA; C427203:calf thymus DNA; D013482:Superoxide Dismutase