title: Antiosteoporotic effects of benzyl benzoate glucosides from Curculigo orchioides in ovariectomized rats.
abstract: OBJECTIVE
  To evaluate the antiosteoporotic effects of benzyl benzoate glucosides from Curculigo orchioides (COBG) in ovariectomized (OVX) rats.
  
  
  METHODS
  A total of 70 female Sprague-Dawley rats were assigned to sham-operated and OVX model groups. The OVX rats were further divided into six subgroups treated by gavage with vehicle, 1 mg/kg of nylestriol, 6, 18 and 54 mg/kg of COBG and 3.0 g/kg of ethanol extract of Curculigo orchioides respectively for 12 weeks. Bone mineral density (BMD) was measured by dual-energy X-ray absorptiometry. The sections of tibia were prepared for histomorphometric analysis. The biomarkers in serum and urine were determined using reagent kits.
  
  
  RESULTS
  Ovariectomy induced the bone loss and microarchitectural deterioration of bone tissue with the activities of increased serum alkaline phosphatase and loss of calcium through the excretion in urine, and decreased levels of antioxidant in serum (P<0.05, P<0.01). Administration of 6, 18 and 54 mg/kg of COBG significantly increased the BMD, improved the microarchitecture of bone tissue, prevented the depletion of antioxidant enzymes like superoxide dismutase and glutathione peroxidase, inhibited the increase of malondialdehyde in serum and reduced the excretion of urine calcium in OVX rats (P<0.05, P<0.01).
  
  
  CONCLUSIONS
  COBG could prevent the bone loss through improving the antioxidant status, which offers a potential new therapeutic drug for postmenopausal osteoporosis.
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Yuan-hui
authors.initials: YH
authors.lastname: Guo
authors.forename: Hai-liang
authors.initials: HL
authors.lastname: Xin
authors.forename: Yan
authors.initials: Y
authors.lastname: Nie
authors.forename: Ting
authors.initials: T
authors.lastname: Han
authors.forename: Lu-ping
authors.initials: LP
authors.lastname: Qin
authors.forename: Qiao-yan
authors.initials: QY
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001565:Benzoates / Q000494:pharmacology*; D015519:Bone Density / Q000187:drug effects; D002118:Calcium / Q000652:urine; D031408:Curculigo / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005260:Female; D005269:Femur / Q000187:drug effects / Q000378:metabolism; D005960:Glucosides / Q000494:pharmacology*; D010024:Osteoporosis / Q000378:metabolism / Q000517:prevention & control*; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D001565:Benzoates; D004365:Drugs, Chinese Herbal; D005960:Glucosides; C006723:benzyl benzoate; D002118:Calcium