title: Dietary fibers reduce food intake by satiation without conditioned taste aversion in mice.
abstract: It is well known that intake of dietary fiber (DF) potently decreases food intake and feelings of hunger and/or promotes satiety ratings. However, the mechanisms explaining these effects are not well characterized. This work was performed to determine which of satiation and/or satiety mechanisms provoke the decrease of food intake induced by DF in mice. We tested in an intra-group protocol a low-viscosity (LV, fructo-oligosaccharide), a viscous (VP, guar gum) and a high-viscosity (HV, mixture of guar gum and fructo-oligosaccharide) preload. These were given to mice by intra-gastric gavage. It appeared that viscous preloads such as VP and HV reduced the daily energy intake by 14% and 21% respectively. The strong effect of HV was mainly due to a large decrease of meal size (by 57%) and meal duration (by 65%) with no effect on ingestion rate during the first 30 min after administration. Therefore, the DF-induced decrease of energy intake was due to a satiation mechanism. This is further supported by a 3-fold increased sensitization of neurons in the nucleus of the solitary tract as observed by c-Fos protein immunolabelling. No compensation of food intake was observed during the rest of the day, a phenomenon that may be explained by the fact that metabolic rate remained high despite the lower food intake. We have also shown that the DF-induced inhibition of food intake was not paired with a conditioned taste aversion. To conclude, this work demonstrates that DF inhibits food intake by increasing satiation during ~1h after administration.
authors.affiliation: AgroParisTech, CRNH-IdF, UMR 914 Nutrition Physiology and Ingestive Behavior, F-75005 Paris, France.
authors.forename: Rojo
authors.initials: R
authors.lastname: Rasoamanana
authors.forename: Patrick C
authors.initials: PC
authors.lastname: Even
authors.forename: Nicolas
authors.initials: N
authors.lastname: Darcel
authors.forename: Daniel
authors.initials: D
authors.lastname: Tomé
authors.forename: Gilles
authors.initials: G
authors.lastname: Fromentin
mesh_terms: D000818:Animals; D001362:Avoidance Learning; D002245:Carbon Dioxide / Q000378:metabolism; D003216:Conditioning, Operant; D003627:Data Interpretation, Statistical; D004032:Diet; D004043:Dietary Fiber / Q000008:administration & dosage / Q000494:pharmacology*; D004435:Eating / Q000187:drug effects*; D004734:Energy Metabolism / Q000187:drug effects; D007150:Immunohistochemistry; D007441:Intubation, Gastrointestinal; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity / Q000502:physiology; D010101:Oxygen Consumption / Q000187:drug effects; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D015656:Respiratory Mechanics / Q000187:drug effects; D012527:Satiation / Q000187:drug effects*; D017552:Solitary Nucleus / Q000187:drug effects / Q000378:metabolism; D013649:Taste / Q000187:drug effects*
chemical_list: D004043:Dietary Fiber; D016760:Proto-Oncogene Proteins c-fos; D002245:Carbon Dioxide