title: Synthesis and cytotoxicity evaluation of novel acylated starch nanoparticles.
abstract: Starch nanoparticles (StNPs) were acylated under ambient conditions to obtain various nanosized derivatives formed stable suspension in water and soluble in organic solvents. The degree of substitution (DS) was determined using (1)H NMR technique. The cytotoxicity potential of the derivatised StNPs was evaluated in mouse embryonic fibroblast (3T3L1) cells and A549 tumor cell line using MTT cell viability assay. Other parameters that determine the oxidative stress viz., reactive oxygen species (ROS) generation, intracellular reduced glutathione (GSH), superoxide generation and acridine orange/ethidium bromide staining were also investigated. The present study led to the conclusion that cytotoxic activity of acylated starch nanoparticles was dependent on their dosage, DS and type of substitution. The non-toxic nature in non-cancerous cells reveals that the nanoparticles (NPs) can be used for cancer therapy and drug delivery. The nanoparticles also offered reasonable binding propensity with CT-DNA.
authors.affiliation: Department of Chemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara 390 002, Gujarat, India. chemistry2797@yahoo.com
authors.forename: Sonal
authors.initials: S
authors.lastname: Thakore
authors.forename: Mayur
authors.initials: M
authors.lastname: Valodkar
authors.forename: Jigar Y
authors.initials: JY
authors.lastname: Soni
authors.forename: Komal
authors.initials: K
authors.lastname: Vyas
authors.forename: Rajendrasinh N
authors.initials: RN
authors.lastname: Jadeja
authors.forename: Ranjitsinh V
authors.initials: RV
authors.lastname: Devkar
authors.forename: Puran Singh
authors.initials: PS
authors.lastname: Rathore
mesh_terms: D041721:3T3-L1 Cells; D000215:Acylation; D000818:Animals; D002417:Cattle; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity*; D006801:Humans; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity* / Q000648:ultrastructure; D013213:Starch / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity*
chemical_list: D004337:Drug Carriers; D013213:Starch; D004247:DNA; C427203:calf thymus DNA