title: Microscale separation of heparosan, heparan sulfate, and heparin.
abstract: The separation and quantification of glycosaminoglycan (GAG) chains with different levels of sulfation from cells and media, and prepared through chemoenzymatic synthesis or metabolic engineering, pose a major challenge in glycomics analysis. A method for microscale separation and quantification of heparin, heparan sulfate, and heparosan from cells is reported. This separation relies on a mini strong anion exchange spin column eluted stepwise with various concentrations of sodium chloride. Disaccharide analysis by LC-MS was used to monitor the chemical structure of the various GAG chains that were recovered.
authors.affiliation: College of Food Science and Technology, Ocean University of China, Qingdao 266003, People's Republic of China.
authors.forename: Xue
authors.initials: X
authors.lastname: Zhao
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.forename: Kathryn
authors.initials: K
authors.lastname: Linkens
authors.forename: Payel
authors.initials: P
authors.lastname: Datta
authors.forename: Akihiro
authors.initials: A
authors.lastname: Onishi
authors.forename: Fuming
authors.initials: F
authors.lastname: Zhang
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Linhardt
mesh_terms: D000818:Animals; D016466:CHO Cells; D002623:Chemistry Techniques, Analytical; D006224:Cricetinae; D004187:Disaccharides / Q000032:analysis* / Q000737:chemistry; D006493:Heparin / Q000032:analysis* / Q000737:chemistry; D006497:Heparan Sulfate / Q000032:analysis* / Q000737:chemistry
chemical_list: D004187:Disaccharides; D006493:Heparin; D006497:Heparan Sulfate; C560363:heparosan