title: A (1)H NMR metabolic profiling to the assessment of protein tyrosine phosphatase 1B role in liver regeneration after partial hepatectomy.
abstract: Protein tyrosine phosphatase 1B (PTP1B) is a negative regulator of the tyrosine kinase growth factor signaling pathway, which is involved in major physiological mechanisms such as liver regeneration. We investigate early hepatic metabolic events produced by partial hepatectomy (PHx) for PTP1B deficient (PTP1B KO) and wild type (WT) mice using proton nuclear magnetic resonance spectroscopy. Metabolic response of the two genotypes produced 24 h upon PHx is compared using magic angle spinning high-resolution nuclear magnetic resonance ((1)H-HR-MAS-NMR) on intact liver tissues. In addition, genotype-associated metabolic profile changes were monitored during the first 48 h after PHx using high-resolution nuclear magnetic resonance ((1)H-HR-NMR) on liver extracts. A marked increase of lipid-related signals in regenerating livers was observed after 24 h PHx in either intact tissues or liver extracts studies. In spite of this common initial metabolic response, results obtained 48 h after PHx on liver extracts indicate a genotype-differential metabolic pattern. This metabolic pattern resulted in line with well known regenerative features such as more sustained cell proliferation, a better management of lipids as energy fuel and lessened liver injury for PTP1B KO mice as compared to WT. Taken together, these findings suggest the metabolic basis to the pivotal role of PTP1B in liver regeneration.
authors.affiliation: Metabolomics Platform, IISPV, Universitat Rovira i Virgili, Avda. Països Catalans, 26, 43007 Tarragona, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Samino
authors.forename: Jesús
authors.initials: J
authors.lastname: Revuelta-Cervantes
authors.forename: Maria
authors.initials: M
authors.lastname: Vinaixa
authors.forename: Miguel Ángel
authors.initials: MÁ
authors.lastname: Rodríguez
authors.forename: Angela M
authors.initials: AM
authors.lastname: Valverde
authors.forename: Xavier
authors.initials: X
authors.lastname: Correig
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D002725:Chloroform / Q000737:chemistry; D055786:Gene Knockout Techniques; D006498:Hepatectomy; D008099:Liver / Q000166:cytology / Q000378:metabolism / Q000601:surgery; D008115:Liver Regeneration; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055442:Metabolome; D000432:Methanol / Q000737:chemistry; D051379:Mice; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D000097:Acetonitriles; D014867:Water; D002725:Chloroform; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D000432:Methanol; C032159:acetonitrile