title: Fluorescent peptide indicator displacement assay for monitoring interactions between RNA and RNA binding proteins.
abstract: This paper describes a sensitive, non-destructive displacement assay, using a fluorescent peptide indicator, for real-time monitoring of the interactions between RNA and RNA binding proteins (RBPs). The developed fluorescent peptide indicators, each containing a mid-sequence fluorophore unit, allowed sensing of target RNA and RNA-RBP interactions through changes in fluorescence intensity. We anticipate that this assay will open up new possibilities for meaningful studies of RNA-RBP interactions.
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang, 790-784, Republic of Korea.
authors.forename: Hyun Seok
authors.initials: HS
authors.lastname: Jeong
authors.forename: Sun Mi
authors.initials: SM
authors.lastname: Choi
authors.forename: Hyun Woo
authors.initials: HW
authors.lastname: Kim
authors.forename: Jung Woo
authors.initials: JW
authors.lastname: Park
authors.forename: Ha Na
authors.initials: HN
authors.lastname: Park
authors.forename: Sung Mi
authors.initials: SM
authors.lastname: Park
authors.forename: Sung Key
authors.initials: SK
authors.lastname: Jang
authors.forename: Young Min
authors.initials: YM
authors.lastname: Rhee
authors.forename: Byeang Hyean
authors.initials: BH
authors.lastname: Kim
mesh_terms: D001483:Base Sequence; D001667:Binding, Competitive; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D007202:Indicators and Reagents / Q000737:chemistry / Q000378:metabolism; D008956:Models, Chemical; D008958:Models, Molecular; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D059372:Nucleotide Motifs; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D010455:Peptides; D016601:RNA-Binding Proteins; D012313:RNA