title: Modulation of Epstein-Barr virus nuclear antigen 2-dependent transcription by protein arginine methyltransferase 5.
abstract: Epstein-Barr Virus Nuclear Antigen (EBNA) 2 features an Arginine-Glycine repeat (RG) domain at amino acid positions 335-360, which is a known target for protein arginine methyltransferaser 5 (PRMT5). In this study, we performed protein affinity pull-down assays to demonstrate that endogenous PRMT5 derived from lymphoblastoid cells specifically associated with the protein bait GST-E2 RG. Transfection of a plasmid expressing PRMT5 induced a 2.5- to 3-fold increase in EBNA2-dependent transcription of both the LMP1 promoter in AKATA cells, which contain the EBV genome endogenously, and a Cp-Luc reporter plasmid in BJAB cells, which are EBV negative. Furthermore, we showed that there was a 2-fold enrichment of EBNA2 occupancy in target promoters in the presence of exogenous PRMT5. Taken together, we show that PRMT5 triggers the symmetric dimethylation of EBNA2 RG domain to coordinate with EBNA2-mediated transcription. This modulation suggests that PRMT5 may play a role in latent EBV infection.
authors.affiliation: Department of Life Sciences, Tzu-Chi University, 701 Chung-Yang Rd. Sec 3, Hualien 97004, Taiwan.
authors.forename: Cheng-Der
authors.initials: CD
authors.lastname: Liu
authors.forename: Chi-Ping
authors.initials: CP
authors.lastname: Cheng
authors.forename: Jia-Shih
authors.initials: JS
authors.lastname: Fang
authors.forename: Ling-Chih
authors.initials: LC
authors.lastname: Chen
authors.forename: Bo
authors.initials: B
authors.lastname: Zhao
authors.forename: Elliott
authors.initials: E
authors.lastname: Kieff
authors.forename: Chih-Wen
authors.initials: CW
authors.lastname: Peng
mesh_terms: D000595:Amino Acid Sequence; D001120:Arginine / Q000737:chemistry; D045744:Cell Line, Tumor; D020031:Epstein-Barr Virus Infections / Q000235:genetics*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000737:chemistry / Q000378:metabolism*; D005786:Gene Expression Regulation; D005998:Glycine / Q000737:chemistry; D004854:Herpesvirus 4, Human / Q000378:metabolism*; D006801:Humans; D008745:Methylation; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D017434:Protein Structure, Tertiary; D011484:Protein-Arginine N-Methyltransferases / Q000378:metabolism*; D020449:Repetitive Sequences, Amino Acid; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: C402641:EBNA-2 protein, Human herpesvirus 4; D019309:Epstein-Barr Virus Nuclear Antigens; D014764:Viral Proteins; D001120:Arginine; C400529:PRMT5 protein, human; D011484:Protein-Arginine N-Methyltransferases; D005998:Glycine