title: Suitability of autologous serum for expanding rabbit adipose-derived stem cell populations.
abstract: Adipose-derived stem cells (ASCs) are believed to have potential use for treating many illnesses. Most cells, including ASCs, are generally cultured in medium containing fetal bovine serum (FBS). However, FBS, which could induce an immune response or infection, is not recommended for clinical applications. In the present study, we evaluated the morphology, proliferation rate, and characterization of rabbit ASCs grown in medium containing autologous serum (AS) and compared these cells to ones cultured with FBS. Morphological changes were monitored by microscopy and flow cytometry. Proliferation rates were assessed with cell counting and ASC phenotypes were characterized by flow cytometry using representative surface markers (CD44 and CD45). Expression of epidermal growth factor, brain-derived neurotrophic factor, and vascular endothelial growth factor was measured by reverse transcription-polymerase chain reaction. Results of our study showed that ASCs had a greater expansion rate in AS without developing morphological heterogeneity than cells grown in FBS. AS-cultured ASCs expressed representative growth factors, CD44 but not CD45, similar to cells cultured in FBS. Expression levels of some growth factors were different between AS and FBS. In conclusion, our findings indicated that AS could potentially be used as a culture medium supplement for the expansion of autologous ASCs.
authors.affiliation: Department of Neurology, Biomedical Research Institute, Seoul National University Hospital, Seoul 110-744, Korea.
authors.forename: Jae-Jun
authors.initials: JJ
authors.lastname: Bahn
authors.forename: Jin-Young
authors.initials: JY
authors.lastname: Chung
authors.forename: Wooseok
authors.initials: W
authors.lastname: Im
authors.forename: Manho
authors.initials: M
authors.lastname: Kim
authors.forename: Sae Hoon
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods / Q000662:veterinary*; D049109:Cell Proliferation; D003470:Culture Media / Q000737:chemistry*; D004815:Epidermal Growth Factor / Q000378:metabolism; D005434:Flow Cytometry / Q000662:veterinary; D018960:Hyaluronan Receptors / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D044967:Serum / Q000737:chemistry*; D013234:Stem Cells / Q000378:metabolism / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D003470:Culture Media; D018960:Hyaluronan Receptors; D042461:Vascular Endothelial Growth Factor A; D004815:Epidermal Growth Factor; D017493:Leukocyte Common Antigens