title: Probing enzymatic activity inside living cells using a nanowire-cell "sandwich" assay.
abstract: Developing a detailed understanding of enzyme function in the context of an intracellular signal transduction pathway requires minimally invasive methods for probing enzyme activity in situ. Here, we describe a new method for monitoring enzyme activity in living cells by sandwiching live cells between two vertical silicon nanowire (NW) arrays. Specifically, we use the first NW array to immobilize the cells and then present enzymatic substrates intracellularly via the second NW array by utilizing the NWs' ability to penetrate cellular membranes without affecting cells' viability or function. This strategy, when coupled with fluorescence microscopy and mass spectrometry, enables intracellular examination of protease, phosphatase, and protein kinase activities, demonstrating the assay's potential in uncovering the physiological roles of various enzymes.
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, South Korea.
authors.forename: Yu-Ran
authors.initials: YR
authors.lastname: Na
authors.forename: So Yeon
authors.initials: SY
authors.lastname: Kim
authors.forename: Jellert T
authors.initials: JT
authors.lastname: Gaublomme
authors.forename: Alex K
authors.initials: AK
authors.lastname: Shalek
authors.forename: Marsela
authors.initials: M
authors.lastname: Jorgolli
authors.forename: Hongkun
authors.initials: H
authors.lastname: Park
authors.forename: Eun Gyeong
authors.initials: EG
authors.lastname: Yang
mesh_terms: D004798:Enzymes / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D013058:Mass Spectrometry; D008856:Microscopy, Fluorescence; D053770:Nanowires
chemical_list: D004798:Enzymes