title: Alba shapes the archaeal genome using a delicate balance of bridging and stiffening the DNA.
abstract: Architectural proteins have an important role in shaping the genome and act as global regulators of gene expression. How these proteins jointly modulate genome plasticity is largely unknown. In archaea, one of the most abundant proteins, Alba, is considered to have a key role in organizing the genome. Here we characterize the multimodal architectural properties and interplay of the Alba1 and Alba2 proteins using single-molecule imaging and manipulation techniques. We demonstrate that the two paralogues can bridge and rigidify DNA and that the interplay between the two proteins influences the balance between these effects. Our data yield a structural model that explains the multimodal behaviour of Alba proteins and its impact on genome folding.
authors.affiliation: Department of Physics and Astronomy, VU University, 1081 HV, Amsterdam, The Netherlands.
authors.forename: Niels
authors.initials: N
authors.lastname: Laurens
authors.forename: Rosalie P C
authors.initials: RP
authors.lastname: Driessen
authors.forename: Iddo
authors.initials: I
authors.lastname: Heller
authors.forename: Daan
authors.initials: D
authors.lastname: Vorselen
authors.forename: Maarten C
authors.initials: MC
authors.lastname: Noom
authors.forename: Felix J H
authors.initials: FJ
authors.lastname: Hol
authors.forename: Malcolm F
authors.initials: MF
authors.lastname: White
authors.forename: Remus T
authors.initials: RT
authors.lastname: Dame
authors.forename: Gijs J L
authors.initials: GJ
authors.lastname: Wuite
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019641:DNA, Archaeal / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020745:Genome, Archaeal; D009690:Nucleic Acid Conformation; D048229:Sulfolobus solfataricus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D019843:Archaeal Proteins; D019641:DNA, Archaeal; D004268:DNA-Binding Proteins