title: Preparation method of lamprey antisera and activity assay.
abstract: Lampreys, the surviving representative of jawless vertebrates, have been a focal point in the search for the evolutionary origin of adaptive immunity. They have independently evolved the variable lymphocyte receptor (VLR)-based adaptive immune system that protects themselves from infection by a variable of microorganisms. The standard immunization schedule for Japanese lamprey (Lampetra japonica) was established to prepare antisera by injection of Escherichia coli, Bacillus proteus, Staphylococcus aureus, Mycobacterium smegmatis, RRBCs, SRBCs, NB4 cells and Hela cells. In this study, we demonstrated the activities of lamprey antisera, which might be helpful to research the collaboration between VLR-based adaptive immune system and complement system in jawless vertebrates.
authors.affiliation: College of Life Science, Liaoning Normal University, Dalian, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Su
authors.forename: Liyong
authors.initials: L
authors.lastname: Chen
authors.forename: Xu
authors.initials: X
authors.lastname: Qiao
authors.forename: Fenfang
authors.initials: F
authors.lastname: Wu
authors.forename: Bo
authors.initials: B
authors.lastname: Feng
authors.forename: Yinglun
authors.initials: Y
authors.lastname: Han
authors.forename: Ge
authors.initials: G
authors.lastname: Liu
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Li
mesh_terms: D056704:Adaptive Immunity; D000371:Agglutination; D000818:Animals; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology; D001433:Bacteriolysis; D045744:Cell Line, Tumor / Q000276:immunology; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D006367:HeLa Cells / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology / Q000302:isolation & purification*; D007115:Immunization Schedule; D007273:Injections, Intramuscular; D007274:Injections, Intraperitoneal; D007798:Lampreys / Q000276:immunology*; D015473:Leukemia, Promyelocytic, Acute / Q000276:immunology / Q000473:pathology; D008297:Male; D020102:Mycobacterium smegmatis / Q000276:immunology; D011511:Proteus / Q000276:immunology; D012756:Sheep; D013211:Staphylococcus aureus / Q000276:immunology
chemical_list: D000941:Antigens; D000939:Epitopes; D007106:Immune Sera