title: Linezolid dependence in Staphylococcus epidermidis bloodstream isolates.
abstract: We document linezolid dependence among 5 highly linezolid-resistant (LRSE) Staphylococcus epidermidis bloodstream isolates that grew substantially faster at 32 µg/mL linezolid presence. These isolates carried the mutations T2504A and C2534T in multiple 23S rRNA copies and 2 mutations leading to relevant amino acid substitutions in L3 protein. Linezolid dependence could account for increasing LRSE emergence.
authors.affiliation: Department of Microbiology, University of Thessaly Medical School, Larissa, Greece. pournaras@med.uth.gr
authors.forename: Spyros
authors.initials: S
authors.lastname: Pournaras
authors.forename: Eleni
authors.initials: E
authors.lastname: Ntokou
authors.forename: Olympia
authors.initials: O
authors.lastname: Zarkotou
authors.forename: Kyriaki
authors.initials: K
authors.lastname: Ranellou
authors.forename: Katerina
authors.initials: K
authors.lastname: Themeli-Digalaki
authors.forename: Constantinos
authors.initials: C
authors.lastname: Stathopoulos
authors.forename: Athanassios
authors.initials: A
authors.lastname: Tsakris
mesh_terms: D000081:Acetamides / Q000378:metabolism / Q000494:pharmacology*; D000328:Adult; D000368:Aged; D019943:Amino Acid Substitution; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D024881:Drug Resistance, Bacterial; D005260:Female; D006115:Greece / Q000453:epidemiology; D006801:Humans; D000069349:Linezolid; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D023303:Oxazolidinones / Q000378:metabolism / Q000494:pharmacology*; D017354:Point Mutation; D012338:RNA, Ribosomal, 23S / Q000235:genetics* / Q000302:isolation & purification; D000090822:Ribosomal Protein L3; D012269:Ribosomal Proteins / Q000235:genetics; D012703:Serotyping; D013203:Staphylococcal Infections / Q000097:blood / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology; D013212:Staphylococcus epidermidis / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000081:Acetamides; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D023303:Oxazolidinones; D012338:RNA, Ribosomal, 23S; D000090822:Ribosomal Protein L3; D012269:Ribosomal Proteins; D000069349:Linezolid