title: Atypical feline sporotrichosis resembling vaccine-induced sarcoma: clinical and histopathological aspects.
abstract: A 7-year-old Siamese cat presenting with three ulcerated cutaneous nodules in the lumbosacral region was seen at the Laboratory for Clinical Research on Dermatozoonoses in Domestic Animals in Rio de Janeiro, Brazil. Histopathological analysis showed that the lesions consisted of polyhedral and spindle-shaped voluminous mononuclear cells with loose chromatin and clearly visible nucleoli, few giant cells, and foci of coagulative and caseous necrosis -- findings suggestive of a vaccine-induced sarcoma. No significant mitotic rate, cytological atypias or asteroid bodies were observed. Special histopathological staining with periodic acid-Schiff and Grocott's silver stain demonstrated the presence of small yeast cells characterized by simple and narrow-base budding compatible with Sporothrix schenckii. Mycological culture grew S schenckii. Cytopathology was negative for yeast cells. These atypical clinical and histopathological signs support the importance of histopathological analysis with special staining techniques, in addition to mycological culture in the diagnosis of feline sporotrichosis.
authors.affiliation: Laboratory for Clinical Research on Dermatozoonoses in Domestic Animals, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. belebarbieri@yahoo.com.br
authors.forename: Isabele Barbieri
authors.initials: IB
authors.lastname: dos Santos
authors.forename: Leonardo Pereira
authors.initials: LP
authors.lastname: Quintella
authors.forename: Luisa Helena Monteiro
authors.initials: LH
authors.lastname: de Miranda
authors.forename: Marcele Nogueira
authors.initials: MN
authors.lastname: de Sousa Trotte
authors.forename: Tânia Maria Pacheco
authors.initials: TM
authors.lastname: Schubach
authors.forename: Rogerio
authors.initials: R
authors.lastname: Tortelly
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000627:therapeutic use; D002371:Cat Diseases / Q000175:diagnosis / Q000188:drug therapy / Q000473:pathology*; D002415:Cats; D005260:Female; D007654:Ketoconazole / Q000627:therapeutic use; D012509:Sarcoma / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D012983:Soft Tissue Neoplasms / Q000473:pathology / Q000662:veterinary*; D013175:Sporothrix / Q000302:isolation & purification; D013174:Sporotrichosis / Q000175:diagnosis / Q000188:drug therapy / Q000473:pathology / Q000662:veterinary*
chemical_list: D000935:Antifungal Agents; D007654:Ketoconazole