title: Rehmannia glutinosa polysaccharide induces maturation of murine bone marrow derived Dendritic cells (BMDCs).
abstract: Purified Rehmannia glutinosa polysaccharide (RGP) is used as functional foods for the prevention and treatment of various diseases. In this study, we examined the effects of RGP on phenotypic and functional maturation of murine bone marrow derived Dendritic cells (BMDCs). Phenotypic maturation of BMDCs was confirmed by conventional scanning electron microscopy (SEM), flow cytometry (FCM) and functional maturation by transmission electron microscopy (TEM), cytochemistry assay, Acid phosphatase (ACP) activity, FITC-dextran, bio-assay and enzyme linked immunosorbent assay (ELISA).We found that RGP up-regulated the expression of CD40, CD80, CD83, CD86 and MHC II molecules of BMDCs, down-regulated pinocytosis and phagocytosis activity, induced IL-12 and TNF-α production of BMDCs. It is therefore concluded that RGP can effectively promote the maturation of DCs. Our study provides evidence and rationale on using RGP in various clinical conditions to enhance host immunity and suggests RGP as a potent adjuvant for the design of DC-based vaccines.
authors.affiliation: Department of Immunology, School of Basic Medical Science, China Medical University, No. 92, North Second Road, Heping District, Shenyang 110001, PR China.
authors.forename: Zhenjie
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yiming
authors.initials: Y
authors.lastname: Meng
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Guo
authors.forename: Xin
authors.initials: X
authors.lastname: He
authors.forename: Qingrui
authors.initials: Q
authors.lastname: Liu
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Wang
authors.forename: Fengping
authors.initials: F
authors.lastname: Shan
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D039521:CD11c Antigen / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D015536:Down-Regulation / Q000235:genetics; D005434:Flow Cytometry; D018664:Interleukin-12 / Q000096:biosynthesis; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D010873:Pinocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000494:pharmacology*; D032267:Rehmannia / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D039521:CD11c Antigen; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D000135:Acid Phosphatase