title: Molecular diagnosis of Leishmania amazonensis in a captive spider monkey in Bauru, São Paulo, Brazil.
abstract: The municipality of Bauru, São Paulo, Brazil, is an area endemic for leishmaniasis. At the zoo, a spider monkey (Ateles paniscus) showed nonpathognomonic symptoms, such as weight loss and pale mucous membranes. Blood was collected from the jugular vein and investigated for the presence of Leishmania spp. DNA by polymerase chain reaction and restriction fragment length polymorphism. Parasite DNA was detected, and the pattern observed was identical to Leishmania amazonensis. This study presents molecular evidence of L. amazonensis infection in a captive spider monkey.
authors.affiliation: Departamento de Clinica, Cirurgia e Reprodução Animal, Faculdade de Odontologia -- Medicina Veterinária, Universidade Estadual Paulista, Aragatuba, São Paulo, Brazil. vmflima@fmva.unesp.br
authors.forename: Valéria Marçal Felix de
authors.initials: VM
authors.lastname: Lima
authors.forename: Maria Emília Bodini
authors.initials: ME
authors.lastname: Santiago
authors.forename: Leticia da Cruz
authors.initials: Lda C
authors.lastname: Sanches
authors.forename: Beatriz Dolabela de
authors.initials: BD
authors.lastname: Lima
mesh_terms: D000818:Animals; D000836:Animals, Zoo; D053819:Atelinae; D001938:Brazil / Q000453:epidemiology; D016054:DNA, Protozoan / Q000235:genetics; D007891:Leishmania / Q000145:classification* / Q000302:isolation & purification*; D007896:Leishmaniasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D008297:Male; D008992:Monkey Diseases / Q000453:epidemiology / Q000469:parasitology*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D016054:DNA, Protozoan