title: Metabolic engineering of Escherichia coli for the production of 5-aminovalerate and glutarate as C5 platform chemicals.
abstract: 5-Aminovalerate (5AVA) is the precursor of valerolactam, a potential building block for producing nylon 5, and is a C5 platform chemical for synthesizing 5-hydroxyvalerate, glutarate, and 1,5-pentanediol. Escherichia coli was metabolically engineered for the production of 5-aminovalerate (5AVA) and glutarate. When the recombinant E. coli WL3110 strain expressing the Pseudomonas putidadavAB genes encoding delta-aminovaleramidase and lysine 2-monooxygenase, respectively, were cultured in a medium containing 20g/L of glucose and 10g/L of L-lysine, 3.6g/L of 5AVA was produced by converting 7g/L of L-lysine. When the davAB genes were introduced into recombinant E. coli strainXQ56allowing enhanced L-lysine synthesis, 0.27 and 0.5g/L of 5AVA were produced directly from glucose by batch and fed-batch cultures, respectively. Further conversion of 5AVA into glutarate could be demonstrated by expression of the P. putida gabTD genes encoding 5AVA aminotransferase and glutarate semialdehyde dehydrogenase. When recombinant E. coli WL3110 strain expressing the davAB and gabTD genes was cultured in a medium containing 20g/L glucose, 10g/L L-lysine and 10g/L α-ketoglutarate, 1.7g/L of glutarate was produced.
authors.affiliation: Department of Environmental Engineering and Energy, Myongji University, San 38-2, Nam-dong, Cheoin-gu, Yongin-si, Gyeonggido 449-728, Republic of Korea.
authors.forename: Si Jae
authors.initials: SJ
authors.lastname: Park
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Kim
authors.forename: Won
authors.initials: W
authors.lastname: Noh
authors.forename: Hye Min
authors.initials: HM
authors.lastname: Park
authors.forename: Young Hoon
authors.initials: YH
authors.lastname: Oh
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Lee
authors.forename: Bong Keun
authors.initials: BK
authors.lastname: Song
authors.forename: Jonggeon
authors.initials: J
authors.lastname: Jegal
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
mesh_terms: D000581:Amidohydrolases / Q000096:biosynthesis / Q000235:genetics; D021542:Amino Acids, Neutral / Q000096:biosynthesis* / Q000235:genetics; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005977:Glutarates / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*; D006899:Mixed Function Oxygenases / Q000096:biosynthesis / Q000235:genetics; D016958:Pseudomonas putida / Q000201:enzymology / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D021542:Amino Acids, Neutral; D001426:Bacterial Proteins; D005977:Glutarates; D011994:Recombinant Proteins; C013809:5-aminovaleric acid; D006899:Mixed Function Oxygenases; C100031:lysine monooxygenase; D000581:Amidohydrolases; C019694:5-aminovaleramidase; C035736:glutaric acid