title: Novel stem-loop probe DNA arrays: detection of specific acetotrophic 16S ribosomal RNA signatures.
abstract: In a recent study, we showed how novel stem-loop DNA probes on dendron-modified aldehyde substrates could be used to detect synthetic nucleic acid targets without amplification. In this article, we demonstrate the application of stem-loop DNA probes as arrays for the detection of specific families and genera of methane-producing bacteria from sludge samples harvested from an anaerobic digester using 16S ribosomal RNA (rRNA) signatures. Specific 16S rRNA could be detected in samples that had 0.2ng/μl total sludge RNA without any target amplification.
authors.affiliation: Department of Chemical Engineering, University of Alabama in Huntsville, Huntsville, AL 35899, USA.
authors.forename: Jonas
authors.initials: J
authors.lastname: Boateng
authors.forename: Robert
authors.initials: R
authors.lastname: Zahorchak
authors.forename: Joel
authors.initials: J
authors.lastname: Peek
authors.forename: Krishnan
authors.initials: K
authors.lastname: Chittur
mesh_terms: D001483:Base Sequence; D019640:Methanosarcinales / Q000235:genetics* / Q000302:isolation & purification; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012329:RNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D012722:Sewage / Q000382:microbiology*
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012722:Sewage