title: Tumor budding, myofibroblast proliferation, and fibrosis in obstructing colon carcinoma: the roles of Hsp47 and basic fibroblast growth factor.
abstract: This study was designed to assess the mechanism of obstruction in obstructing colorectal carcinomas. Thirty-five cases of obstructing colorectal carcinoma and 34 cases of non-obstructing carcinoma were studied. The lesions were immunohistochemically analyzed using antibodies for pan-cytokeratin, α-smooth muscle actin, matrix metalloproteinase-7, 47-kDa heat shock protein (Hsp47), basic fibroblast growth factor (bFGF), myeloperoxidase, and CD68. Compared with non-obstructing cases, obstructing carcinoma cases included lesions of poorer differentiation. A higher value of tumor budding was observed in obstructing than in non-obstructing carcinoma. A higher number of α-smooth muscle actin-positive myofibroblasts, a higher expression of Hsp47 in stromal spindle cells, and a higher expression of bFGF in inflammatory cells were also significant in obstructing carcinoma. Therefore, obstructing colon carcinomas were characterized by poorer differentiation of cancer cells, a high level of tumor budding, and stromal myofibroblast proliferation resulting in fibrosis. Correlative Hsp47 expression in fibroblasts with bFGF in inflammatory cells may contribute to stromal fibrosis.
authors.affiliation: Department of Pathology, Kitasato University School of Medicine, Sagamihara, Japan.
authors.forename: Chuan-Jie
authors.initials: CJ
authors.lastname: Xu
authors.forename: Tetuo
authors.initials: T
authors.lastname: Mikami
authors.forename: Takatoshi
authors.initials: T
authors.lastname: Nakamura
authors.forename: Tomoko
authors.initials: T
authors.lastname: Tsuruta
authors.forename: Norihiro
authors.initials: N
authors.lastname: Nakada
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Yanagisawa
authors.forename: Shi-Xu
authors.initials: SX
authors.lastname: Jiang
authors.forename: Isao
authors.initials: I
authors.lastname: Okayasu
mesh_terms: D000368:Aged; D002277:Carcinoma / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D003110:Colonic Neoplasms / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000032:analysis / Q000378:metabolism*; D005355:Fibrosis / Q000473:pathology; D050958:HSP47 Heat-Shock Proteins / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007415:Intestinal Obstruction / Q000209:etiology / Q000378:metabolism; D008297:Male; D058628:Myofibroblasts / Q000473:pathology*; D060787:Neoplasm Grading; D009367:Neoplasm Staging
chemical_list: D050958:HSP47 Heat-Shock Proteins; D016222:Fibroblast Growth Factor 2