title: Transient filament occlusion of the middle cerebral artery in rats: does the reperfusion method matter 24 hours after perfusion?
abstract: BACKGROUND
  There are two widely used transient middle cerebral artery occlusion (MCAO) methods, which differ in the use of unilateral or bilateral carotid artery reperfusion (UNICAR and BICAR). Of the two methods, UNICAR is easier to perform. This study was designed to comprehensively compare the two reperfusion methods to determine if there are any differences in outcomes.
  
  
  RESULTS
  The UNICAR and BICAR groups each included 9 rats. At baseline, the average pO(2) was 20.54 ± 9.35 and 26.43 ± 7.39, for the UNICAR and BICAR groups, respectively (P = 0.519). Changes in pO(2), as well as other physiological parameters measured within the ischemic lesion, were similar between the UNICAR and BICAR groups during 90 min of MCAO and the first 30 min of reperfusion (all P > 0.05). Furthermore, both the Bederson score and Garcia score, which are used for neurological assessment, were also similar (both P > 0.05). There were also no significant differences in T2WI lesion volume, DWI lesion volume, PWI lesion volume, or TTC staining infarct volume between the two groups (all P > 0.05).
  
  
  CONCLUSIONS
  UNICAR and BICAR have similar capability for inducing acute brain ischemic injury and can be considered interchangeable up to 24 hours after reperfusion.
authors.affiliation: Department of Neurology, University of Schleswig-Holstein, Campus Kiel, Christian-Albrechts-University Kiel, Kiel, Germany. liujianren0571@hotmail.com
authors.forename: Jian-Ren
authors.initials: JR
authors.lastname: Liu
authors.forename: Ulf R
authors.initials: UR
authors.lastname: Jensen-Kondering
authors.forename: Jia-Jun
authors.initials: JJ
authors.lastname: Zhou
authors.forename: Fen
authors.initials: F
authors.lastname: Sun
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Feng
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Shen
authors.forename: Günther
authors.initials: G
authors.lastname: Deuschl
authors.forename: Olav
authors.initials: O
authors.lastname: Jansen
authors.forename: Thomas
authors.initials: T
authors.lastname: Herdegen
authors.forename: Johannes
authors.initials: J
authors.lastname: Meyne
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhao
authors.forename: Christoph
authors.initials: C
authors.lastname: Eschenfelder
mesh_terms: D000818:Animals; D002560:Cerebrovascular Circulation / Q000502:physiology*; D038524:Diffusion Magnetic Resonance Imaging / Q000379:methods; D004195:Disease Models, Animal; D020244:Infarction, Middle Cerebral Artery / Q000401:mortality / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D059906:Neuroimaging / Q000379:methods; D009460:Neurologic Examination; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion / Q000379:methods*; D015427:Reperfusion Injury / Q000401:mortality / Q000473:pathology* / Q000503:physiopathology; D012720:Severity of Illness Index; D013997:Time Factors