title: The sequence variation responsible for the functional difference between the CONSTANS protein, and the CONSTANS-like (COL) 1 and COL2 proteins, resides mostly in the region encoded by their first exons.
abstract: Although the protein CONSTANS (CO) and its close relatives CONSTANS-like (COL) 1 and COL2 exhibit high amino acid sequence similarities, only the CO protein regulates floral induction in Arabidopsis. To investigate the structural basis for the functional differences between CO, COL1, and COL2 in flowering, we performed domain-swapping between CO, COL1, and COL2, and site-directed mutagenesis on the first exon of CO. The results suggest that the lack of flowering promotion activity by COL1 and COL2 is mainly attributed to the differences between CO and the COL1 and COL2 proteins in the amino acid sequence encoded by their first exons.
authors.affiliation: Plant Signaling Network Research Center, Division of Life Sciences, Korea University, Seoul, Republic of Korea.
authors.forename: Soon-Kap
authors.initials: SK
authors.lastname: Kim
authors.forename: Hyo-Young
authors.initials: HY
authors.lastname: Park
authors.forename: Yun Hee
authors.initials: YH
authors.lastname: Jang
authors.forename: Jeong Hwan
authors.initials: JH
authors.lastname: Lee
authors.forename: Jeong-Kook
authors.initials: JK
authors.lastname: Kim
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D019143:Evolution, Molecular; D005091:Exons / Q000235:genetics*; D035264:Flowers / Q000235:genetics* / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D014644:Genetic Variation; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D030821:Plants, Genetically Modified; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins; D016415:Sequence Alignment; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D029681:Arabidopsis Proteins; C092776:CONSTANS protein, Arabidopsis; D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors