title: Femtogram ultrasensitive aptasensor for the detection of Ochratoxin A.
abstract: A simple and ultrasensitive method was developed for the detection of Ochratoxin A, utilizing an aptamer as a molecular recognition probe and real-time quantitative PCR (RT-qPCR) amplification of its complementary DNA as signal generators. Under the optimized conditions, the cycle threshold (Ct) increased linearly with 10-fold serial dilutions of Ochratoxin A (OTA) from 5×10⁻⁶ to 5 ng mL⁻¹, with a limit of detection (LOD) of 1 fg mL⁻¹. The specificity of this aptasensor was considered to be excellent, as when tested against four other toxins it produced no obvious Ct value change. Furthermore, a satisfactory analyte concentration recovery was obtained from a series of concentrations of OTA spiked into red wine. Therefore, this highly sensitive approach shows a significant potential in a wide range of target analytes.
authors.affiliation: School of Food Science and Technology, State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.forename: Honghong
authors.initials: H
authors.lastname: Yin
authors.forename: Liguang
authors.initials: L
authors.lastname: Xu
authors.forename: Zhou
authors.initials: Z
authors.lastname: Xu
authors.forename: Hua
authors.initials: H
authors.lastname: Kuang
authors.forename: Libing
authors.initials: L
authors.lastname: Wang
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Xu
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry; D006801:Humans; D009793:Ochratoxins / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction; D014920:Wine / Q000032:analysis
chemical_list: D052157:Aptamers, Nucleotide; D009793:Ochratoxins; C025589:ochratoxin A; D004247:DNA