title: Imparting regenerative capacity to limbs by progenitor cell transplantation.
abstract: The frog Xenopus can normally regenerate its limbs at early developmental stages but loses the ability during metamorphosis. This behavior provides a potential gain-of-function model for measures that can enhance limb regeneration. Here, we show that frog limbs can be caused to form multidigit regenerates after receiving transplants of larval limb progenitor cells. It is necessary to activate Wnt/β-catenin signaling in the cells and to add Sonic hedgehog, FGF10, and thymosin β4. These factors promote survival and growth of the grafted cells and also provide pattern information. The eventual regenerates are not composed solely of donor tissue; the host cells also make a substantial contribution despite their lack of regeneration competence. Cells from adult frog legs or from regenerating tadpole tails do not promote limb regeneration, demonstrating the necessity for limb progenitor cells. These findings have obvious implications for the development of a technology to promote limb regeneration in mammals.
authors.affiliation: Stem Cell Institute, University of Minnesota, 2001 6(th) Street S.E., Minneapolis, MN 55455, USA. linxx519@umn.edu
authors.forename: Gufa
authors.initials: G
authors.lastname: Lin
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Jonathan M W
authors.initials: JM
authors.lastname: Slack
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D017209:Apoptosis; D002454:Cell Differentiation; D049109:Cell Proliferation; D017690:Cell Transplantation; D005121:Extremities / Q000502:physiology* / Q000637:transplantation; D005260:Female; D051526:Fibroblast Growth Factor 10 / Q000378:metabolism; D053823:Hedgehog Proteins / Q000378:metabolism; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D007814:Larva / Q000254:growth & development* / Q000378:metabolism; D012038:Regeneration / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D013934:Thymectomy; D013947:Thymosin / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism / Q000601:surgery; D051153:Wnt Proteins / Q000378:metabolism; D029867:Xenopus Proteins / Q000378:metabolism; D014982:Xenopus laevis / Q000254:growth & development* / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism; D000099201:Thymosin beta(4)
chemical_list: D051526:Fibroblast Growth Factor 10; D053823:Hedgehog Proteins; D013947:Thymosin; D051153:Wnt Proteins; D029867:Xenopus Proteins; D051176:beta Catenin; D000099201:Thymosin beta(4); C496466:fgf10 protein, Xenopus