title: NGF-induced neurite outgrowth in PC12 cells is independent of calcium entry through L-type calcium channels.
abstract: Neuronal growth factor (NGF) induces neurodifferentiation of PC12 cells into cholinergic neurons-like cells. It was shown that intracellular Ca2+ ions participate in regulation of the differentiation of PC12 cells. We tested whether L-type calcium channels contribute to Ca2+ entry which supports neurite outgrowth accompanying NGF-activated differentiation process. Development of morphological changes did correlate with increase of functional expression of L-type calcium channels. However, inhibition of L-type calcium channels by 1 μM of isradipine did not affect significantly an NGF-activated neurite outgrowth.
authors.affiliation: Institute of Molecular Physiology and Genetics, VVCE Biomembranes, Slovak Academy of Sciences, Bratislava, Slovak Republic. lucia.lichvarova@savba.sk
authors.forename: Lucia
authors.initials: L
authors.lastname: Lichvárová
authors.forename: Katarína
authors.initials: K
authors.lastname: Jašková
authors.forename: L'ubica
authors.initials: L
authors.lastname: Lacinová
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020746:Calcium Channels, L-Type / Q000187:drug effects / Q000502:physiology*; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology*; D020932:Nerve Growth Factor / Q000008:administration & dosage*; D016501:Neurites / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure*; D009474:Neurons; D016716:PC12 Cells; D051381:Rats
chemical_list: D020746:Calcium Channels, L-Type; D020932:Nerve Growth Factor; D002118:Calcium