title: Sphingopyxis italica sp. nov., isolated from Roman catacombs.
abstract: A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, strain SC13E-S71(T), was isolated from tuff, volcanic rock, where the Roman catacombs of Saint Callixtus in Rome, Italy, was excavated. Analysis of 16S rRNA gene sequences revealed that strain SC13E-S71(T) belongs to the genus Sphingopyxis, and that it shows the greatest sequence similarity with Sphingopyxis chilensis DSM 14889(T) (98.72 %), Sphingopyxis taejonensis DSM 15583(T) (98.65 %), Sphingopyxis ginsengisoli LMG 23390(T) (98.16 %), Sphingopyxis panaciterrae KCTC 12580(T) (98.09 %), Sphingopyxis alaskensis DSM 13593(T) (98.09 %), Sphingopyxis witflariensis DSM 14551(T) (98.09 %), Sphingopyxis bauzanensis DSM 22271(T) (98.02 %), Sphingopyxis granuli KCTC 12209(T) (97.73 %), Sphingopyxis macrogoltabida KACC 10927(T) (97.49 %), Sphingopyxis ummariensis DSM 24316(T) (97.37 %) and Sphingopyxis panaciterrulae KCTC 22112(T) (97.09 %). The predominant fatty acids were C18 : 1ω7c, summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), C14 : 0 2-OH and C16 : 0. The predominant menaquinone was MK-10. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and sphingoglycolipid. These chemotaxonomic data are common to members of the genus Sphingopyxis. However, a polyphasic approach using physiological tests, DNA base ratios, DNA-DNA hybridization and 16S rRNA gene sequence comparisons showed that the isolate SC13E-S71(T) belongs to a novel species within the genus Sphingopyxis, for which the name Sphingopyxis italica sp. nov. is proposed. The type strain is SC13E-S71(T) ( = DSM 25229(T) = CECT 8016(T)).
authors.affiliation: Instituto de Recursos Naturales y Agrobiologia, IRNAS-CSIC, Apartado 1052, 41080 Sevilla, Spain.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Alias-Villegas
authors.affiliation: Instituto de Recursos Naturales y Agrobiologia, IRNAS-CSIC, Apartado 1052, 41080 Sevilla, Spain.
authors.forename: Valme
authors.initials: V
authors.lastname: Jurado
authors.affiliation: Instituto de Recursos Naturales y Agrobiologia, IRNAS-CSIC, Apartado 1052, 41080 Sevilla, Spain.
authors.forename: Leonila
authors.initials: L
authors.lastname: Laiz
authors.affiliation: Instituto de Recursos Naturales y Agrobiologia, IRNAS-CSIC, Apartado 1052, 41080 Sevilla, Spain.
authors.forename: Cesareo
authors.initials: C
authors.lastname: Saiz-Jimenez
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012384:Rome; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C024989:coenzyme Q10