title: Ganglioside GM1 influences the proliferation rate of mouse induced pluripotent stem cells.
abstract: Gangliosides play important roles in the control of several biological processes, including proliferation and transmembrane signaling. In this study, we demonstrate the effect of ganglioside GM1 on the proliferation of mouse induced pluripotent stem cells (miPSCs). The proliferation rate of miPSCs was lower than in mouse embryonic stem cells (mESCs). Fluorescence activated cell sorting analysis showed that the percentage of cells in the G2/M phase in miPSCs was lower than that in mESCs. GM1 was expressed in mESCs, but not miPSCs. To confirm the role of GM1 in miPSC proliferation, miPSCs were treated with GM1. GM1-treated miPSCs exhibited increased cell proliferation and a larger number of cells in the G2/M phase. Furthermore, phosphorylation of mitogen-activated protein kinases was increased in GM1- treated miPSCs.
authors.affiliation: Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan 570-749, Korea.
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Ryu
authors.forename: Kyu-Tae
authors.initials: KT
authors.lastname: Chang
authors.forename: Ju-Taek
authors.initials: JT
authors.lastname: Lee
authors.forename: Malg-Um
authors.initials: MU
authors.lastname: Lim
authors.forename: Hyun-Ki
authors.initials: HK
authors.lastname: Min
authors.forename: Yoon-Ju
authors.initials: YJ
authors.lastname: Na
authors.forename: Su-Bin
authors.initials: SB
authors.lastname: Lee
authors.forename: Gislain
authors.initials: G
authors.lastname: Moussavou
authors.forename: Sun-Uk
authors.initials: SU
authors.lastname: Kim
authors.forename: Ji-Su
authors.initials: JS
authors.lastname: Kim
authors.forename: Kinarm
authors.initials: K
authors.lastname: Ko
authors.forename: Kisung
authors.initials: K
authors.lastname: Ko
authors.forename: Kyung-A
authors.initials: KA
authors.lastname: Hwang
authors.forename: Eun-Jeong
authors.initials: EJ
authors.lastname: Jeong
authors.forename: Jeong-Woong
authors.initials: JW
authors.lastname: Lee
authors.forename: Young-Kug
authors.initials: YK
authors.lastname: Choo
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D005434:Flow Cytometry; D005677:G(M1) Ganglioside / Q000494:pharmacology*; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D059566:M Phase Cell Cycle Checkpoints / Q000187:drug effects; D020935:MAP Kinase Signaling System; D051379:Mice
chemical_list: D005677:G(M1) Ganglioside