title: Preparation of cell-culturing glass surfaces that release branched polyethyleneimine triggered by thiol-disulfide exchange.
abstract: To develop a chemical stimulus-responsive substrate for culturing cells, polyethyleneimine (PEI) having a pyridyl disulfide moiety was attached via disulfide linkages to a glass coverslip modified with a silane coupling agent having a thiol group. The surface modification was confirmed by X-ray photoelectron spectroscopy and zeta potential analysis. The obtained surface exhibited sufficiently high cell adhesiveness. Zeta potential measurements revealed that the PEI derivatives were released from the surface through thiol-disulfide exchange when the modified glass coverslip was immersed in a neutral pH buffer containing cysteine. The cell viability assay demonstrated that this chemical stimulus was substantially nontoxic to 293T cells. Because PEI is a widely used transfection reagent, this functional glass coverslip would be potentially useful as an experimental platform for reverse transfection.
authors.affiliation: Bioengineering Laboratory, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. akimoto@cross.t.u-tokyo.ac.jp
authors.forename: Aya Mizutani
authors.initials: AM
authors.lastname: Akimoto
authors.forename: Tohru
authors.initials: T
authors.lastname: Takarada
authors.forename: Mizuo
authors.initials: M
authors.lastname: Maeda
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival; D002478:Cells, Cultured; D004220:Disulfides / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D006801:Humans; D011094:Polyethyleneimine / Q000737:chemistry*; D055672:Static Electricity; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D004220:Disulfides; D013438:Sulfhydryl Compounds; D011094:Polyethyleneimine