title: Immunopontentiating and antitumor activities of a polysaccharide from Pulsatilla chinensis (Bunge) Regel.
abstract: One water-soluble polysaccharide (PCPw) was isolated and purified from the roots of Pulsatilla chinensis by DEAE cellulose-52 and Sephadex G-100 column chromatography, and its antitumor activity was evaluated on 4T1 tumor-bearing mice through transplantable animal tumor. After 10 days of PCPw (50, 100 and 200 mg/kg) treatment once daily in tumor-bearing mice, PCPw oral administration could not only significantly inhibit the growth of transplantable 4T1 tumor in mice but also promote concanavalin A (Con A), lipopolysaccharide (LPS)-stimulated splenocytes proliferation, the serum lysozyme level and 2,4-dinitrofluorobenzene (DNFB)-induced delayed-type hypersensitivity (DTH) reactions, especially at the dose of 100 mg/kg. Meanwhile, significant improvements in peripheral blood abnormality and anemia were observed in PCPw-treated group. These results suggested that PCPw could improve both cellular and humoral immune response and might be explored as a potential natural antitumor drug.
authors.affiliation: Department of Breast Surgery, The Third Affiliated (Tumour) Hospital of Harbin Medical University, Haping Road, No. 150, Harbin 150040, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Liu
authors.forename: Leiguang
authors.initials: L
authors.lastname: Ye
authors.forename: Xueqing
authors.initials: X
authors.lastname: Guan
authors.forename: Xiaoshuan
authors.initials: X
authors.lastname: Liang
authors.forename: Cong
authors.initials: C
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Sun
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Shiru
authors.initials: S
authors.lastname: Chen
authors.forename: Fa
authors.initials: F
authors.lastname: Bang
authors.forename: Baogang
authors.initials: B
authors.lastname: Liu
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003208:Concanavalin A / Q000494:pharmacology; D004139:Dinitrofluorobenzene; D005260:Female; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology / Q000473:pathology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009113:Muramidase / Q000097:blood; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D031946:Pulsatilla / Q000737:chemistry*; D013154:Spleen / Q000187:drug effects / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
chemical_list: D000276:Adjuvants, Immunologic; D000970:Antineoplastic Agents; D008070:Lipopolysaccharides; D011134:Polysaccharides; D003208:Concanavalin A; D004139:Dinitrofluorobenzene; D009113:Muramidase