title: Effects of voluntary wheel running on the kidney at baseline and after ischaemia-reperfusion-induced acute kidney injury: a strain difference comparison.
abstract: Exercise-induced vascular endothelial adaptations in the kidney are not well understood. Therefore, we investigated the impact of voluntary wheel running (VWR) on the abundance of endothelial nitric oxide synthase (eNOS) and extracellular superoxide dismutase (EC SOD), in kidney and lung, and other SOD isoforms and total antioxidant capacity (TAC), in kidney. We also determined whether VWR influences susceptibility to acute kidney injury (AKI). Male Sprague-Dawley and Fisher 344 rats, VWR or sedentary for 12 weeks, were subjected to AKI (uninephrectomy (UNX) and 35 min of left kidney ischaemia-24 h reperfusion, IR). We measured glomerular filtration rate (GFR) and renal plasma flow (RPF), and analysed renal structural injury. Running was comparable between strains and VWR reduced body weight. In Sprague-Dawley rats, VWR reduced eNOS and EC SOD, but increased Mn SOD in kidney. Similar changes were seen after 6 weeks of VWR in Sprague-Dawley rats. In Fisher 344 rats, VWR increased eNOS, all SOD isoforms and TAC in kidney. Both strains increased eNOS and EC SOD in lung with VWR. Compared to UNX alone, UNX-IR injury markedly reduced renal function for both strains; however, in the Sprague-Dawley rats, VWR exacerbated falls in GFR and RPF due to UNX-IR, whereas in the Fisher 344 rats, GFR was unaffected by VWR. Some indices of renal structural injury due to UNX-IR tended to be worse in SD vs. F344. Our study demonstrates that genetic background influences the effect of exercise on kidney eNOS and EC SOD, which in turn influence the susceptibility to AKI.
authors.affiliation: Department of Cellular & Molecular Physiology, Yale University, P.O. Box 208026, New Haven, CT 208026, USA. natasha.moningka@yale.edu
authors.forename: Natasha C
authors.initials: NC
authors.lastname: Moningka
authors.forename: Mark W
authors.initials: MW
authors.lastname: Cunningham
authors.forename: Myrline
authors.initials: M
authors.lastname: Sterling
authors.forename: Crystal A
authors.initials: CA
authors.lastname: West
authors.forename: Jill W
authors.initials: JW
authors.lastname: Verlander
authors.forename: Byron P
authors.initials: BP
authors.lastname: Croker
authors.forename: Joslyn
authors.initials: J
authors.lastname: Ahlgren
authors.forename: Linda
authors.initials: L
authors.lastname: Hayward
authors.forename: Chris
authors.initials: C
authors.lastname: Baylis
mesh_terms: D058186:Acute Kidney Injury / Q000209:etiology* / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004195:Disease Models, Animal; D005838:Genotype; D005919:Glomerular Filtration Rate; D007668:Kidney / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D008168:Lung / Q000378:metabolism; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D010641:Phenotype; D005082:Physical Exertion; D051381:Rats; D011916:Rats, Inbred F344; D017207:Rats, Sprague-Dawley; D017595:Renal Plasma Flow; D015427:Reperfusion Injury / Q000209:etiology* / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D012420:Running; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors; D014836:Volition
chemical_list: D000975:Antioxidants; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat; D013482:Superoxide Dismutase