title: Hyaluronan production and chondrogenic properties of primary human chondrocyte on gelatin based hematostatic spongostan scaffold.
abstract: BACKGROUND
  Autologous chondrocyte transplantation is a promising technique for treatment of cartilage defects. Three dimensional chondrocyte cultures on a scaffold are widely used to retain the chondrogenic phenotype. Using a biodegradable gelatin scaffold is one option for the cell delivery system, but molecular and histological studies of the method have not yet been done.
  
  
  METHODS
  We evaluated the chondrogenic property of the primary human chondrocyte on a gelatin scaffold as compared to a collagen scaffold over a period of 21 days. We examined the production of glycosaminoglycan by quantitative and histological analysis. Gene expression of cartilage-associated molecules was assessed by quantitative RT-PCR.
  
  
  RESULTS
  The gelatin scaffold showed the ability to promote chondrocyte expansion, chondrogenic phenotype retention at molecular and mRNA levels.
  
  
  CONCLUSIONS
  This scaffold is thus suitable for use as an in vitro model for chondrocyte 3D culture.
authors.affiliation: Thailand Excellence Center for Tissue Engineering and Stem Cells, Faculty of Medicine, Chiang Mai University, Intravarorot Road, Sripoom, Chiang Mai, 50200, Thailand.
authors.forename: Jeerawan
authors.initials: J
authors.lastname: Klangjorhor
authors.forename: Puwapong
authors.initials: P
authors.lastname: Nimkingratana
authors.forename: Jongkolnee
authors.initials: J
authors.lastname: Settakorn
authors.forename: Dumnoensun
authors.initials: D
authors.lastname: Pruksakorn
authors.forename: Taninnit
authors.initials: T
authors.lastname: Leerapun
authors.forename: Olarn
authors.initials: O
authors.lastname: Arpornchayanon
authors.forename: Sattaya
authors.initials: S
authors.lastname: Rojanasthien
authors.forename: Prachya
authors.initials: P
authors.lastname: Kongtawelert
authors.forename: Peraphan
authors.initials: P
authors.lastname: Pothacharoen
mesh_terms: D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D020219:Chondrogenesis / Q000187:drug effects*; D003094:Collagen / Q000494:pharmacology; D005780:Gelatin / Q000494:pharmacology*; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D054457:Tissue Scaffolds; D055815:Young Adult
chemical_list: D006025:Glycosaminoglycans; D012333:RNA, Messenger; D005780:Gelatin; D006820:Hyaluronic Acid; D003094:Collagen