title: Parameters optimization of supercritical fluid-CO2 extracts of frankincense using response surface methodology and its pharmacodynamics effects.
abstract: The volatile oil parts of frankincense (Boswellia carterii Birdw.) were extracted with supercritical carbon dioxide under constant pressure (15, 20, or 25 MPa) and fixed temperature (40, 50, or 60°C), given time (60, 90, or 120 min) aiming at the acquisition of enriched fractions containing octyl acetate, compounds of pharmaceutical interest. A mathematical model was created by Box-Behnken design, a popular template for response surface methodology, for the extraction process. The response value was characterized by synthetical score, which comprised yields accounting for 20% and content of octyl acetate for 80%. The content of octyl acetate was determined by GC. The supercritical fluid extraction showed higher selectivity than conventional steam distillation. Supercritical fluid-CO(2)  for extracting frankincense under optimum condition was of great validity, which was also successfully verified by the pharmacological experiments.
authors.affiliation: Separation Engineering of Chinese Traditional Medicine Compound, Nanjing University of Chinese Medicine, Nanjing, P. R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.forename: Xing-miao
authors.initials: XM
authors.lastname: Ma
authors.forename: Bi-Han
authors.initials: BH
authors.lastname: Qiu
authors.forename: Jun-xia
authors.initials: JX
authors.lastname: Chen
authors.forename: Lin
authors.initials: L
authors.lastname: Bian
authors.forename: Lin-mei
authors.initials: LM
authors.lastname: Pan
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology; D029743:Boswellia / Q000737:chemistry*; D002245:Carbon Dioxide; D002849:Chromatography, Gas; D025924:Chromatography, Supercritical Fluid / Q000295:instrumentation / Q000379:methods*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000188:drug therapy; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009822:Oils, Volatile / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D010938:Plant Oils / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D009822:Oils, Volatile; D010938:Plant Oils; D002245:Carbon Dioxide