title: Involvement of transcription initiation factor IIB in the light-induced death of rat retinal ganglion cells in vivo.
abstract: Transcription initiation factor IIB (TFIIB) is a general transcription initiation factor that plays a pivotal role in the response to transcriptional activator proteins. Previous reports have shown that TFIIB have been implicated in the pathogenesis of various experimental central nervous system diseases. However, its distribution and function in the retina remain unclear. In the present study, we investigated the spatiotemporal expression of TFIIB in a light-induced retinal damage model. Western blotting analysis showed TFIIB level significantly improved 3 days after injury, and then declined during the following days. The association of TFIIB and retinal ganglion cells (RGCs) was detected by immunofluorescence double staining. The injury-induced expression of TFIIB was physically co-existed with active caspase-3 and TUNEL (apoptotic markers). Spatiotemporal changes of TFIIB expression suggest that this protein may play a role in the degenerative process of RGCs by light-induced damage in the retina.
authors.affiliation: Department of Ophthalmology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, People's Republic of China.
authors.forename: Aimin
authors.initials: A
authors.lastname: Sang
authors.forename: Yue
authors.initials: Y
authors.lastname: Xu
authors.forename: Nan
authors.initials: N
authors.lastname: Jin
authors.forename: Tianqiu
authors.initials: T
authors.lastname: Zhou
authors.forename: Junjun
authors.initials: J
authors.lastname: Wang
authors.forename: Juming
authors.initials: J
authors.lastname: Zhu
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Shi
authors.forename: Jie
authors.initials: J
authors.lastname: Shuai
authors.forename: Guofeng
authors.initials: G
authors.lastname: Xu
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Gu
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D016923:Cell Death; D005260:Female; D008027:Light; D008297:Male; D021381:Protein Transport; D051381:Rats; D012165:Retinal Ganglion Cells / Q000378:metabolism*; D035581:Transcription Factor TFIIB / Q000378:metabolism*
chemical_list: D035581:Transcription Factor TFIIB; D053148:Caspase 3