title: A gDNA microarray for genotyping salvia species.
abstract: Salvia is an important genus from the Lamiaceae with approximately 1,000 species. This genus is distributed globally and cultivated for ornamental, culinary, and medicinal uses. We report the construction of the first fingerprinting array for Salvia species enriched with polymorphic and divergent DNA sequences and demonstrate the potential of this array for fingerprinting several economically important members of this genus. In order to generate the Salvia subtracted diversity array (SDA) a suppression subtractive hybridization (SSH) was performed between a pool of Salvia species and a pool of angiosperms and non-angiosperms to selectively isolate Salvia-specific sequences. A total of 285-subtracted genomic DNA (gDNA) fragments were amplified and arrayed. DNA fingerprints were obtained for fifteen Salvia genotypes including three that were not part of the original subtraction pool. Hierarchical cluster analysis indicated that the Salvia-specific SDA was capable of differentiating S. officinalis and S. miltiorrhiza from their closely related species and was also able to reveal genetic relationships consistent with geographical origins. In addition, this approach was capable of isolating highly polymorphic sequences from chloroplast and nuclear DNA without preliminary sequence information. Therefore, SDA is a powerful technique for fingerprinting non-model plants and for identifying new polymorphic loci that may be developed as potential molecular markers.
authors.affiliation: Health Innovations Research Institute, School of Applied Sciences, RMIT University, Melbourne, VIC 3000, Australia. alolarte27@yahoo.com
authors.forename: Alexandra
authors.initials: A
authors.lastname: Olarte
authors.forename: Nitin
authors.initials: N
authors.lastname: Mantri
authors.forename: Gregory
authors.initials: G
authors.lastname: Nugent
authors.forename: Hans
authors.initials: H
authors.lastname: Wohlmuth
authors.forename: Chun Guang
authors.initials: CG
authors.lastname: Li
authors.forename: Charlie
authors.initials: C
authors.lastname: Xue
authors.forename: Edwin
authors.initials: E
authors.lastname: Pang
mesh_terms: D018744:DNA, Plant / Q000032:analysis / Q000235:genetics*; D015723:Gene Library; D018745:Genome, Plant; D060005:Genotyping Techniques / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010802:Phylogeny; D010944:Plants; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D027544:Salvia / Q000145:classification / Q000235:genetics*
chemical_list: D018744:DNA, Plant