title: Evaluation of low background liquid scintillation counter for non-clinical ADME studies.
abstract: 1. The performance of low background (BG) liquid scintillation counter (LSC) was evaluated for practical purposes of non-clinical drug absorption, distribution, metabolism and excretion (ADME) studies. The measurement conditions for the radioactivity for low BG LSC were investigated and metabolite profiling in rat plasma after single oral administration of (14)C-labeled compounds was performed. Metabolite profiling was also conducted using conventional LSC and accelerator mass spectrometer (AMS), and the performances of these measuring instruments were compared. 2. The established measurement conditions showed good linearity of the calibration curve over the concentration range from 3 to 300 dpm/vial. Metabolite profiling using low BG LSC for the plasma samples diluted to 5-55 times was comparable to that using conventional LSC for the undiluted plasma samples. Meanwhile, metabolite profiling using AMS for the plasma samples diluted to 250-2000 times was comparable to that using conventional LSC. 3. These results suggest that the application of low BG LSC is one of the useful tools for non-clinical ADME studies and that it is possible to select conventional LSC, low BG LSC or AMS for radioactivity measurement by considering the specific radioactivity of the compounds and the predicted concentrations of radioactivity in biological samples in ADME studies.
authors.affiliation: Drug Metabolism and Pharmacokinetics Research Laboratories, Pharmaceutical Research Division, Takeda Pharmaceutical Company Limited, Kanagawa, Japan. akemi.onishi@takeda.com
authors.forename: Akemi
authors.initials: A
authors.lastname: Onishi
authors.forename: Akifumi
authors.initials: A
authors.lastname: Kogame
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Tagawa
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kondo
authors.forename: Satoru
authors.initials: S
authors.lastname: Asahi
mesh_terms: D000042:Absorption; D000818:Animals; D002138:Calibration; D002250:Carbon Radioisotopes / Q000008:administration & dosage / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D057230:Limit of Detection; D008297:Male; D055432:Metabolomics; D004364:Pharmaceutical Preparations / Q000097:blood / Q000378:metabolism*; D011851:Radioactivity; D051381:Rats; D012015:Reference Standards; D012588:Scintillation Counting / Q000379:methods*; D014018:Tissue Distribution
chemical_list: D002250:Carbon Radioisotopes; D004364:Pharmaceutical Preparations