title: Serodiagnostic potential of Mycobacterium avium MAV2054 and MAV5183 proteins.
abstract: The Mycobacterium avium-M. intracellulare complex (MAC) causes a pulmonary disease (PD) similar to tuberculosis (TB). Diagnosis of MAC-PD is complicated and time-consuming. In this study, the serodiagnostic potential of the newly identified MAV2054 and MAV5183 proteins was evaluated in subjects with MAC-PD, pulmonary TB, or latent TB and in noninfected healthy controls (HC), together with HspX and the 38-kDa antigen, well-known serodiagnostic M. tuberculosis antigens. All four antigens evoked significantly higher IgG responses in MAC-PD and active TB than in latent TB and HC subjects. Among the antigens, MAV2054 elicited the highest antibody responses in pulmonary TB and MAC-PD patients. IgG titers against MAV2054 and MAV5183 were significantly higher in MAC-PD than in pulmonary TB subjects. In addition, the levels of IgG against all antigens in the M. intracellulare and fibrocavitary forms were higher than those in the M. avium and nodular bronchiectatic forms, respectively. Based on sensitivity and receiver operator characteristic curve analysis, the best candidates for detection of MAC-PD and pulmonary TB were MAV2054 and the 38-kDa antigen, respectively. In total, 76.0% of MAC-PD and 65.0% of active TB patients were reactive to at least two antigens. In contrast, only 2.8% of HC subjects were reactive with two or more antigens. Our findings suggest that an enzyme-linked immunosorbent assay (ELISA) using the four antigens would be valuable for screening for mycobacterial lung disease, including MAC-PD and pulmonary TB, although it does not provide good discrimination of the disease-causing pathogens.
authors.affiliation: Department of Microbiology and Research Institute for Medical Sciences, Infection Signaling Network Research Center, College of Medicine, Chungnam National University, Daejeon, South Korea.
authors.forename: A-Rum
authors.initials: AR
authors.lastname: Shin
authors.forename: Kil-Soo
authors.initials: KS
authors.lastname: Lee
authors.forename: Kang In
authors.initials: KI
authors.lastname: Lee
authors.forename: Tae Sun
authors.initials: TS
authors.lastname: Shim
authors.forename: Won-Jung
authors.initials: WJ
authors.lastname: Koh
authors.forename: Haet Sal
authors.initials: HS
authors.lastname: Jeon
authors.forename: Yeo-Jin
authors.initials: YJ
authors.lastname: Son
authors.forename: Sung-Jae
authors.initials: SJ
authors.lastname: Shin
authors.forename: Hwa-Jung
authors.initials: HJ
authors.lastname: Kim
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008074:Lipoproteins / Q000276:immunology; D008297:Male; D008875:Middle Aged; D015269:Mycobacterium avium Complex / Q000276:immunology*; D015270:Mycobacterium avium-intracellulare Infection / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D012372:ROC Curve; D011994:Recombinant Proteins / Q000276:immunology; D014376:Tuberculosis / Q000097:blood / Q000175:diagnosis
chemical_list: C075966:Ag38 antigen, Mycobacterium tuberculosis; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C118127:HspX protein, Mycobacterium tuberculosis; D007074:Immunoglobulin G; D008074:Lipoproteins; D011994:Recombinant Proteins