title: iSLIM: a comprehensive approach to mapping and characterizing gene regulatory networks.
abstract: Mapping gene regulatory networks is a significant challenge in systems biology, yet only a few methods are currently capable of systems-level identification of transcription factors (TFs) that bind a specific regulatory element. We developed a microfluidic method for integrated systems-level interaction mapping of TF-DNA interactions, generating and interrogating an array of 423 full-length Drosophila TFs. With integrated systems-level interaction mapping, it is now possible to rapidly and quantitatively map gene regulatory networks of higher eukaryotes.
authors.affiliation: Laboratory of Biological Network Characterization, Institute of Bioengineering, School of Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Rockel
authors.forename: Marcel
authors.initials: M
authors.lastname: Geertz
authors.forename: Korneel
authors.initials: K
authors.lastname: Hens
authors.forename: Bart
authors.initials: B
authors.lastname: Deplancke
authors.forename: Sebastian J
authors.initials: SJ
authors.lastname: Maerkl
mesh_terms: D000818:Animals; D001483:Base Sequence; D016384:Consensus Sequence; D004247:DNA / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics; D015723:Gene Library; D053263:Gene Regulatory Networks; D046210:Microfluidic Analytical Techniques; D059372:Nucleotide Motifs; D056510:Position-Specific Scoring Matrices; D050436:Regulatory Elements, Transcriptional; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: D014157:Transcription Factors; D004247:DNA