title: Adiponectin in mice with altered GH action: links to insulin sensitivity and longevity?
abstract: Adiponectin is positively correlated with longevity and negatively correlated with many obesity-related diseases. While there are several circulating forms of adiponectin, the high-molecular-weight (HMW) version has been suggested to have the predominant bioactivity. Adiponectin gene expression and cognate serum protein levels are of particular interest in mice with altered GH signaling as these mice exhibit extremes in obesity that are positively associated with insulin sensitivity and lifespan as opposed to the typical negative association of these factors. While a few studies have reported total adiponectin levels in young adult mice with altered GH signaling, much remains unresolved, including changes in adiponectin levels with advancing age, proportion of total adiponectin in the HMW form, adipose depot of origin, and differential effects of GH vs IGF1. Therefore, the purpose of this study was to address these issues using assorted mouse lines with altered GH signaling. Our results show that adiponectin is generally negatively associated with GH activity, regardless of age. Further, the amount of HMW adiponectin is consistently linked with the level of total adiponectin and not necessarily with previously reported lifespan or insulin sensitivity of these mice. Interestingly, circulating adiponectin levels correlated strongly with inguinal fat mass, implying that the effects of GH on adiponectin are depot specific. Interestingly, rbGH, but not IGF1, decreased circulating total and HMW adiponectin levels. Taken together, these results fill important gaps in the literature related to GH and adiponectin and question the frequently reported associations of total and HMW adiponectin with insulin sensitivity and longevity.
authors.affiliation: Edison Biotechnology Institute, College of Health Sciences and Professions, E338 Grover Center, Ohio University, Athens, Ohio 45701, USA.
authors.forename: Ellen R
authors.initials: ER
authors.lastname: Lubbers
authors.forename: Edward O
authors.initials: EO
authors.lastname: List
authors.forename: Adam
authors.initials: A
authors.lastname: Jara
authors.forename: Lucila
authors.initials: L
authors.lastname: Sackman-Sala
authors.forename: Jose
authors.initials: J
authors.lastname: Cordoba-Chacon
authors.forename: Manuel D
authors.initials: MD
authors.lastname: Gahete
authors.forename: Rhonda D
authors.initials: RD
authors.lastname: Kineman
authors.forename: Ravneet
authors.initials: R
authors.lastname: Boparai
authors.forename: Andrzej
authors.initials: A
authors.lastname: Bartke
authors.forename: John J
authors.initials: JJ
authors.lastname: Kopchick
authors.forename: Darlene E
authors.initials: DE
authors.lastname: Berryman
mesh_terms: D052242:Adiponectin / Q000097:blood*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001823:Body Composition / Q000502:physiology*; D013006:Growth Hormone / Q000494:pharmacology; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000502:physiology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D020738:Leptin / Q000097:blood; D008136:Longevity / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D011986:Receptors, Somatotropin / Q000235:genetics* / Q000378:metabolism
chemical_list: D052242:Adiponectin; D001786:Blood Glucose; D007328:Insulin; D020738:Leptin; D011986:Receptors, Somatotropin; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone