title: Systemic treatment with vanadium absorbed by Coprinus comatus promotes femoral fracture healing in streptozotocin-diabetic rats.
abstract: The purpose of this study was to analyze the impact of vanadium absorbed by Coprinus comatus (VACC) on fracture healing in streptozotocin-diabetic rats. Forty-five male Wistar rats used were divided into three groups: normal rats (control), diabetic rats, and diabetic rats treated with VACC. A standardized fracture-healing model with a stable plate fixation was established for the rat femoral fracture. After a 4-week stable fixation, callus quality was assessed by microcomputerized tomography and histological and biomechanical examinations. In addition, bone samples were obtained to evaluate the content of mineral substances in bones. Compared with the diabetic group, vanadium treatment significantly increased bone mineral content and biomechanical strength and improved microstructural properties of the callus. The ultimate load was increased by 29.1 % (P<0.05), and the total bone volume of callus enhanced by 11.2 % (P<0.05) at 4 weeks post fracture. Vanadium also promoted callus bone formation, which caused a 35.5 % increase in the total area of callus. However, VACC did not accelerate the fracture repair process in histological analysis. In conclusion, the current study suggests that systemic treatment with vanadium could promote fracture healing in streptozotocin-diabetic rats.
authors.affiliation: Department of Orthopaedics, Shengjing Hospital, China Medical University, Shenyang, Liaoning Province, 110004, People's Republic of China.
authors.forename: Guangbin
authors.initials: G
authors.lastname: Wang
authors.forename: Jiashi
authors.initials: J
authors.lastname: Wang
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Fu
authors.forename: Lunhao
authors.initials: L
authors.lastname: Bai
authors.forename: Ming
authors.initials: M
authors.lastname: He
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Qin
authors.initials: Q
authors.lastname: Fu
mesh_terms: D000818:Animals; D003302:Coprinus / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D005264:Femoral Fractures / Q000150:complications* / Q000473:pathology / Q000628:therapy*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin; D014639:Vanadium / Q000378:metabolism* / Q000627:therapeutic use*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D014639:Vanadium; D013311:Streptozocin