title: Biological markers in cerebrospinal fluid for axonal impairment in multiple sclerosis: acetylcholinesterase activity cannot be considered a useful biomarker.
abstract: An impairment of the cholinergic system activity has been demonstrated in multiple sclerosis (MS). The correlation between the cholinergic system and the cognitive dysfunction in MS has led to studies on the use of acetylcholinesterase inhibitors (AChEI). The acetylcholinesterase (AChE), essential enzyme for the regulation of turnover of acetylcholine, can be considered the most important biochemical indicator of cholinergic signaling in the nervous system. Besides its catalytic properties, AChE has a crucial role in the regulation of the immune function. Based on the role of the AChe in the regulation of cholinergic signaling in the nervous system, the aim of the present study is to evaluate the activity of AChE in different pathological conditions: MS, other inflammatory neurological disorders (OIND) and non-inflammatory neurological disorders (NIND). We measured AChE activity in CSF samples obtained from 34 relapsing-remitting MS patients and, as controls, 40 patients with other inflammatory neurological disorders (OIND) and 40 subjects with other non-inflammatory neurological disorders (NIND). Fluorimetric detection of the AChE in MS patients and in the controls showed no statistically significant differences: 1.507 ± 0.403 nmol/ml/min in MS patients, 1.484 ± 0.496 nmol/ml/min in OIND and 1.305 ± 0.504 nmol/ml/min in NIND. Similar results were obtained in another recent study, using a different method. Further studies must be conducted on a larger number of patients, with different degrees of cognitive impairment. However, AChE measured in CSF can probably not be considered a useful biomarker for the assessment of the functional alterations of cholinergic system in pathological conditions.
authors.affiliation: Section of Pharmacology, Department of Clinical and Experimental Medicine, Ferrara University, Via Fossato di Mortara 17, 44100 Ferrara, Italy.
authors.forename: T
authors.initials: T
authors.lastname: Antonelli
authors.forename: M C
authors.initials: MC
authors.lastname: Tomasini
authors.forename: M
authors.initials: M
authors.lastname: Castellazzi
authors.forename: P
authors.initials: P
authors.lastname: Sola
authors.forename: C
authors.initials: C
authors.lastname: Tamborino
authors.forename: D
authors.initials: D
authors.lastname: Ferraro
authors.forename: L
authors.initials: L
authors.lastname: Ferraro
authors.forename: E
authors.initials: E
authors.lastname: Granieri
mesh_terms: D000110:Acetylcholinesterase / Q000134:cerebrospinal fluid*; D000328:Adult; D001369:Axons / Q000473:pathology*; D015415:Biomarkers / Q000134:cerebrospinal fluid*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid* / Q000473:pathology*; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid
chemical_list: D015415:Biomarkers; D000110:Acetylcholinesterase