title: Expression of NRF2 and NRF2-modulated genes in peripheral blood leukocytes of bladder cancer males.
abstract: Nuclear factor (erythroid-derived 2)-like 2 (NRF2) is an oxidant-responsive transcription factor involved in induction of antioxidant genes. We assessed NRF2 and selected NRF2-modulated gene expression: glutathione S-transferase A1 and P1 (GSTA1 and GSTP1), mitochondrial superoxide dismutase (SOD2) in blood leukocytes of 51 bladder cancer patients and 90 control males. A significant up-regulation of SOD2 expression (P=0.002) was observed in leukocytes of patients. NRF2 expression was positively correlated with GSTP1 and with SOD2 mRNA level, both in patients and controls. These data suggest disturbances in SOD2 transcription in circulating blood leukocytes of males with bladder cancer. Moreover, concomitant constitutive expression of NRF2 and its target genes may suggest important role of NRF2 transcription factor in positive regulation of antioxidant genes, resulted in enhanced cytoprotection in human peripheral blood leukocytes.
authors.affiliation: Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Lodz, Poland. edyta@imp.lodz.pl
authors.forename: E
authors.initials: E
authors.lastname: Reszka
authors.forename: Z
authors.initials: Z
authors.lastname: Jablonowski
authors.forename: E
authors.initials: E
authors.lastname: Wieczorek
authors.forename: J
authors.initials: J
authors.lastname: Gromadzinska
authors.forename: E
authors.initials: E
authors.lastname: Jablonska
authors.forename: M
authors.initials: M
authors.lastname: Sosnowski
authors.forename: W
authors.initials: W
authors.lastname: Wasowicz
mesh_terms: D000368:Aged; D015972:Gene Expression Regulation, Neoplastic; D051549:Glutathione S-Transferase pi / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D051267:NF-E2-Related Factor 2 / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D013482:Superoxide Dismutase / Q000235:genetics; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000378:metabolism; D000099156:Superoxide Dismutase 2
chemical_list: D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D051267:NF-E2-Related Factor 2; D012333:RNA, Messenger; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2; C495635:NFE2L2 protein, human; C484555:GSTA1 protein, human; C496556:GSTP1 protein, human