title: Oral administration of attenuated Salmonella typhimurium containing a DNA vaccine against rabbit haemorrhagic disease.
abstract: The use of attenuated Salmonella typhimurium as a bactofection vehicle for the oral delivery of a DNA vaccine against rabbit haemorrhagic disease virus (RHDV) was investigated. The DNA vaccine plasmid pcDNA3.1-VP60, which encodes the viral capsid protein VP60, was transformed into the attenuated S. typhimurium strain SL7207. The resulting recombinant bacteria, named as SL/pcDNA3.1-VP60, were orally used to immunise rabbits. The successful delivery of the DNA plasmid was confirmed by the detected VP60 transcription in the rabbit intestines through the reverse transcription polymerase chain reaction. In addition, the RHDV-specific humoral and cell-mediated immune response that was induced by SL/pcDNA3.1-VP60 was detected by the enzyme-linked immunosorbent assay as well as the assays for T lymphocyte proliferation and cytokines secretion. The significant protection of immunised rabbits against the RHDV strain XA/China/2010 at 42 d post-immunisation was demonstrated. This study is the first report about the efficient usage of attenuated Salmonella as a live vector for the oral delivery of a DNA vaccine against RHDV.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
authors.forename: Li
authors.initials: L
authors.lastname: Qiu
authors.forename: Xinglong
authors.initials: X
authors.lastname: Wang
authors.forename: Huafang
authors.initials: H
authors.lastname: Hao
authors.forename: Guohui
authors.initials: G
authors.lastname: Mu
authors.forename: Ruyi
authors.initials: R
authors.lastname: Dang
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.forename: Shuxia
authors.initials: S
authors.lastname: Zhang
authors.forename: Enqi
authors.initials: E
authors.lastname: Du
authors.forename: Zengqi
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D017250:Caliciviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D049109:Cell Proliferation; D004337:Drug Carriers / Q000008:administration & dosage*; D017929:Hemorrhagic Disease Virus, Rabbit / Q000235:genetics / Q000276:immunology*; D011817:Rabbits; D012376:Rodent Diseases / Q000276:immunology / Q000517:prevention & control*; D012486:Salmonella typhimurium / Q000235:genetics* / Q000472:pathogenicity; D013601:T-Lymphocytes / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D004337:Drug Carriers; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; D014765:Viral Vaccines