title: Upgrading fungal gene expression on demand: improved systems for doxycycline-dependent silencing in Aspergillus fumigatus.
abstract: Conditional gene expression is key for functional studies in any given microorganism. To allow tight regulation in the pathogenic mold Aspergillus fumigatus, improved versions of the doxycycline-dependent Tet-On system were generated by replacing functional elements of the precursor module, thereby circumventing the former problem of leakiness due to intramolecular recombination.
authors.affiliation: Max von Pettenkofer-Institut, Ludwig-Maximilians-Universität, Munich, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Helmschrott
authors.forename: Anna
authors.initials: A
authors.lastname: Sasse
authors.forename: Sweta
authors.initials: S
authors.lastname: Samantaray
authors.forename: Sven
authors.initials: S
authors.lastname: Krappmann
authors.forename: Johannes
authors.initials: J
authors.lastname: Wagener
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000235:genetics*; D004318:Doxycycline / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D005827:Genetics, Microbial / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D004318:Doxycycline