title: Administration of nicotinamide does not increase platelet levels in mice.
abstract: Elucidating ways to enhance megakaryopoiesis in vivo would have therapeutic applications for thrombocytopenia and transfusion medicine. Nicotinamide has been shown to enhance endomitosis in megakaryocytes cultured in vitro, suggesting that it may be beneficial for the production of platelets in culture. We hypothesized that regular injections of nicotinamide in mice would also increase platelets in vivo. However, we found that platelet counts were reduced by about 25% with daily injections of nicotinamide. Altering the schedule, duration, or nicotinamide dose did not improve platelet production. Consistent with lower platelet levels, nicotinamide also tended to decrease megakaryocyte frequency in sternum and spleen sections, as well as colony formation in vitro by bone marrow progenitor cells. However, there was no effect on the fraction or ploidy of CD41(+) cells harvested from bone marrow. Together, our results suggest that, although nicotinamide increases polyploidization of megakaryocytes in culture, it does not have translatable effects in vivo.
authors.affiliation: Chemical and Biological Engineering Department, Northwestern University, Evanston, IL 60208, USA.
authors.forename: Iwona M
authors.initials: IM
authors.lastname: Konieczna
authors.forename: Swapna
authors.initials: S
authors.lastname: Panuganti
authors.forename: Teresa A
authors.initials: TA
authors.lastname: DeLuca
authors.forename: E Terry
authors.initials: ET
authors.lastname: Papoutsakis
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Eklund
authors.forename: William M
authors.initials: WM
authors.lastname: Miller
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000187:drug effects*; D001854:Bone Marrow Cells / Q000166:cytology; D005260:Female; D008297:Male; D008533:Megakaryocytes / Q000166:cytology; D051379:Mice; D023461:Myeloid Progenitor Cells / Q000187:drug effects; D009536:Niacinamide / Q000008:administration & dosage*; D010976:Platelet Count; D013154:Spleen / Q000166:cytology; D034061:Thrombopoiesis / Q000187:drug effects
chemical_list: D009536:Niacinamide