title: Source-sink dynamics and proteomic reprogramming under elevated night temperature and their impact on rice yield and grain quality.
abstract: High night temperatures (HNTs) can reduce significantly the global rice (Oryza sativa) yield and quality. A systematic analysis of HNT response at the physiological and molecular levels was performed under field conditions. Contrasting rice accessions, N22 (highly tolerant) and Gharib (susceptible), were evaluated at 22°C (control) and 28°C (HNT). Nitrogen (N) and nonstructural carbohydrate (NSC) translocation from different plant tissues into grains at key developmental stages, and their contribution to yield, grain-filling dynamics and quality aspects, were evaluated. Proteomic profiling of flag leaf and spikelets at 100% flowering and 12 d after flowering was conducted, and their reprogramming patterns were explored. Grain yield reduction in susceptible Gharib was traced back to the significant reduction in N and NSC translocation after flowering, resulting in reduced maximum and mean grain-filling rate, grain weight and grain quality. A combined increase in heat shock proteins (HSPs), Ca signaling proteins and efficient protein modification and repair mechanisms (particularly at the early grain-filling stage) enhanced N22 tolerance for HNT. The increased rate of grain filling and efficient proteomic protection, fueled by better assimilate translocation, overcome HNT tolerance in rice. Temporal and spatial proteome programming alters dynamically between key developmental stages and guides future transgenic and molecular analysis targeted towards crop improvement.
authors.affiliation: College of Agronomy, Hunan Agricultural University, Changsha, Hunan, 410128, China.|Crop and Environmental Sciences Division, International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines.
authors.forename: Wanju
authors.initials: W
authors.lastname: Shi
authors.affiliation: Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
authors.forename: Raveendran
authors.initials: R
authors.lastname: Muthurajan
authors.affiliation: Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
authors.forename: Hifzur
authors.initials: H
authors.lastname: Rahman
authors.affiliation: Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
authors.forename: Jagadeesh
authors.initials: J
authors.lastname: Selvam
authors.affiliation: MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
authors.forename: Shaobing
authors.initials: S
authors.lastname: Peng
authors.affiliation: College of Agronomy, Hunan Agricultural University, Changsha, Hunan, 410128, China.
authors.forename: Yinbin
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Crop and Environmental Sciences Division, International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines.
authors.forename: Krishna S V
authors.initials: KSV
authors.lastname: Jagadish
mesh_terms: D018533:Biomass; D020013:Calcium Signaling; D018506:Gene Expression Regulation, Plant; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D012275:Oryza / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome; D013312:Stress, Physiological; D013696:Temperature; D013997:Time Factors
chemical_list: D006360:Heat-Shock Proteins; D010940:Plant Proteins; D020543:Proteome