title: Quantitative analysis of oleic acid and three types of polyethers according to the number of hydroxy end groups in Polysorbate 80 by hydrophilic interaction chromatography at critical conditions.
abstract: A quantitative characterization of Polysorbate 80 is crucial for its many applications. In this paper we report a quick RP-HPLC method for the quantitative determination of Polysorbate 80. The hydrolysis of Polysorbate 80 to release oleic acid and three types of polyethers was first carried out. A chromatographic method based on liquid chromatography at critical conditions (LCCC) was then developed for an endgroup-based separation of low-molecular-mass polyether. With this method the polyether, irrespective of its molecular-mass, is separated according to endgroups (functionality) due to interactions of the polar endgroups with the hydrophilic stationary phase. The different types of polyethers and oleic acid were identified using on-line electrospray ionization mass spectrometry and quantified by evaporative light scattering detection.
authors.affiliation: Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, No. 188 Xingwan Rd, Nanchang 330004, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Ji
authors.forename: Bei-jia
authors.initials: BJ
authors.lastname: Shi
authors.forename: Zai-ping
authors.initials: ZP
authors.lastname: Zhang
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.forename: Yong-mei
authors.initials: YM
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004987:Ethers / Q000032:analysis*; D057927:Hydrophobic and Hydrophilic Interactions; D017665:Hydroxyl Radical; D019301:Oleic Acid / Q000032:analysis*; D011092:Polyethylene Glycols / Q000737:chemistry; D011136:Polysorbates / Q000138:chemical synthesis / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D004987:Ethers; D011136:Polysorbates; D019301:Oleic Acid; D017665:Hydroxyl Radical; D011092:Polyethylene Glycols