title: A new and simple resonance Rayleigh scattering method for human serum albumin using graphite oxide as probe.
abstract: Graphite oxide (GO) was prepared by the Hummer procedure, and can be dispersed to stable colloid solution by ultrasonic wave. The GO exhibited an absorption peak at 313 nm, and a resonance Rayleigh scattering (RRS) peak at 490 nm. In pH 4.6 HAc-NaAc buffer solution, human serum albumin (HSA) combined with GO probe to form large HSA-GO particles that caused the RRS peak increasing at 490 nm. The increased RRS intensity was linear to HSA concentration in the range 0.50-200 µg/mL. Thus, a new and simple RRS method was proposed for the determination of HSA in samples, with a recovery of 98.1-104%.
authors.affiliation: State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Science and Technology of China, Guilin, China; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education of China, Guilin, China; Guangxi Normal University, Guilin, China.
authors.forename: Shengmian
authors.initials: S
authors.lastname: Wang
authors.forename: Lili
authors.initials: L
authors.lastname: Xu
authors.forename: Lisheng
authors.initials: L
authors.lastname: Wang
authors.forename: Aihui
authors.initials: A
authors.lastname: Liang
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D006108:Graphite / Q000737:chemistry*; D006801:Humans; D010087:Oxides / Q000737:chemistry*; D012542:Scattering, Radiation; D012709:Serum Albumin / Q000032:analysis*; D013050:Spectrometry, Fluorescence
chemical_list: D010087:Oxides; D012709:Serum Albumin; D006108:Graphite