title: Developmental expression of P2X5 receptors in the mouse prenatal central and peripheral nervous systems.
abstract: The functions of P2X purinoceptors (P2X1-7) in the nervous system of adults have been widely studied. However, little is known about their roles during embryonic development. Our previous work has reported an extensive expression of P2X5 receptors in the adult mouse central nervous system. In the present study, we have examined the expression pattern of P2X5 receptor mRNA and protein during prenatal development of the mouse nervous system (from embryonic day E8 to E17). P2X5 receptors appeared in the neural tube as early as E8 and were gradually confined to new-born neurons in the cortical plate and ventral horn of the spinal cord. Heavy signals for P2X5 receptors were also found in dorsal root ganglia (DRG), retina, olfactory epithelium, and nerve fibers in skeletal muscles. In conclusion, P2X5 receptors were strongly represented in the developing mouse nervous system. The transient high expression pattern of P2X5 receptors in epithelium-like structures suggests a role during early neurogenesis.
authors.affiliation: Key Laboratory of Medical Neurobiology, Ministry of Health, Department of Neurobiology, School of Medicine, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.forename: Zhaohuan
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Liu
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Burnstock
authors.forename: Zhenghua
authors.initials: Z
authors.lastname: Xiang
authors.forename: Cheng
authors.initials: C
authors.lastname: He
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002490:Central Nervous System / Q000196:embryology*; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000502:physiology; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D055495:Neurogenesis / Q000502:physiology*; D017933:Peripheral Nervous System / Q000196:embryology*; D058485:Receptors, Purinergic P2X5 / Q000032:analysis / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D058485:Receptors, Purinergic P2X5