title: Standardization of microfluidic cell cultures using integrated organic photodiodes and electrode arrays.
abstract: Nanotechnology provides the tools to develop novel biosensors with improved performance, including sensitivity and response time that can be readily integrated into diagnostic devices. We have developed a miniaturized cell analysis platform to advance microfluidic cell cultures by combining two complementary, label-free and non-invasive cell analysis methods for the long-term monitoring of dynamic cell behavior. The novel dual-parameter cell-on-a-chip detects light scattering from adherent cells to provide information on cell numbers and intracellular granularity, while simultaneously performing impedance spectroscopy to monitor cell adhesion and cell-cell interaction. In the present work we have integrated spray-coated organic photodiode arrays with a lab-on-a-chip containing embedded interdigitated electrode structures to improve assay reproducibility, reliability and accuracy. We successfully demonstrate that the complementary cell chip technology can accurately detect cell numbers, clarify misleading results during cell-substance interaction assays, as well as the cytotoxicity screening of drug substances. The ability to precisely determine cell numbers within minutes constitutes a major step towards standardization.
authors.affiliation: AIT Austrian Institute of Technology GmbH, Vienna, Austria.
authors.forename: Verena
authors.initials: V
authors.lastname: Charwat
authors.forename: Michaela
authors.initials: M
authors.lastname: Purtscher
authors.forename: Sandro F
authors.initials: SF
authors.lastname: Tedde
authors.forename: Oliver
authors.initials: O
authors.lastname: Hayden
authors.forename: Peter
authors.initials: P
authors.lastname: Ertl
mesh_terms: D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000592:standards*; D002460:Cell Line; D002470:Cell Survival; D058266:Dielectric Spectroscopy; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004566:Electrodes; D006367:HeLa Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D019169:Jurkat Cells; D008027:Light; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods / Q000592:standards*; D036103:Nanotechnology; D012542:Scattering, Radiation; D017971:Tin Compounds / Q000737:chemistry
chemical_list: D004129:Dimethylpolysiloxanes; D017971:Tin Compounds; C013830:baysilon; C109984:indium tin oxide