title: The 14-3-3 protein GENERAL REGULATORY FACTOR11 (GRF11) acts downstream of nitric oxide to regulate iron acquisition in Arabidopsis thaliana.
abstract: Here we report the function of a general regulatory factor, GENERAL REGULATORY FACTOR11 (GRF11), in terms of the iron (Fe) deficiency response. Physiological and molecular responses of the loss-of-function Arabidopsis thaliana grf11 mutant to Fe supply were investigated. Genes involved in posttranscriptional regulation of FER-LIKE FE DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (FIT) were also analyzed. In addition, the molecular link between the signaling molecule nitric oxide (NO) and Fe deficiency responses was further dissected. Our results suggest that GRF11 is necessary for induction of Fe-deficiency-tolerance mechanisms. The FIT protein can bind to the promoter of GRF11, which contains an E-box motif. GRF11 also positively affects FIT transcription but has no influence on the genes involved in posttranscriptional regulation of FIT. Furthermore, NO positively regulates GRF11 induction upon the onset of Fe deficiency. We propose that, upon the onset of Fe deficiency, induction of FIT expression is dependent on GRF11, which acts downstream of NO to mediate Fe deficiency responses.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Jian Li
authors.initials: JL
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.|College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.
authors.forename: Wei Wei
authors.initials: WW
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Li Qian
authors.initials: LQ
authors.lastname: Chen
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Qin
authors.affiliation: College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Chong Wei
authors.initials: CW
authors.lastname: Jin
authors.affiliation: Tea Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Tea Chemical Engineering, Ministry of Agriculture, Hangzhou, 310008, China.
authors.forename: Yuan Zhi
authors.initials: YZ
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Shao Jian
authors.initials: SJ
authors.lastname: Zheng
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D027682:Cation Transport Proteins / Q000378:metabolism; D038181:FMN Reductase / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D055786:Gene Knockout Techniques; D007501:Iron / Q000378:metabolism*; D008954:Models, Biological; D016254:Mutagenesis, Insertional; D050901:Nitrate Reductase / Q000235:genetics; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000235:genetics; D010641:Phenotype; D030821:Plants, Genetically Modified / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000378:metabolism
chemical_list: D048948:14-3-3 Proteins; D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; D027682:Cation Transport Proteins; D038181:FMN Reductase; D007501:Iron; D050901:Nitrate Reductase; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D006180:Proton-Translocating ATPases; C545099:AHA7 protein, Arabidopsis; C501411:FIT1 protein, Arabidopsis; C581789:GENERAL REGULATORY FACTOR11, Arabidopsis; C099651:IRT1 protein, Arabidopsis; C521947:NOA1 protein, Arabidopsis; C023903:ferric citrate iron reductase; C544444:AHA1 protein, Arabidopsis