title: Kallikrein-related peptidase 10 expression in salivary gland tissues and tumours.
abstract: OBJECTIVE
  Kallikrein-related peptidase 10 (KLK10) has been implicated in the development of several types of cancer. The purpose of this study was to analyze the expression of KLK10 in 3 types of salivary gland tumour and normal salivary glands.
  
  
  METHODS
  A standard immunoperoxidase staining technique was used to assess the immunoexpression profile of KLK10 in normal salivary glands and 3 types of salivary gland tumour: pleomorphic adenoma, adenoid cystic carcinoma and mucoepidermoid carcinoma.
  
  
  RESULTS
  Pleomorphic adenomas showed significantly lower KLK10 levels than control tissues. Neither of the malignant tumours (adenoid cystic carcinoma and mucoepidermoid carcinoma) showed a significant alteration in the immunoreactive scores of KLK10 in comparison with the normal salivary gland tissues. KLK10 immunoreactive scores were comparable in adenoid cystic carcinoma and mucoepidermoid carcinoma. Pleomorphic adenoma had significantly lower levels of KLK10 than mucoepidermoid carcinoma.
  
  
  CONCLUSIONS
  The finding of lower KLK10 levels in pleomorphic adenoma suggests aberrant expression in a tumour that develops primarily from myoepithelial cells. A kallikrein cascade may play a role in the development and/or outcome of some salivary gland tumours.
authors.affiliation: Department of Pathology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada. mark.darling@schulich.uwo.ca
authors.forename: Mark R
authors.initials: MR
authors.lastname: Darling
authors.forename: Nelly N
authors.initials: NN
authors.lastname: Hashem
authors.forename: Irene
authors.initials: I
authors.lastname: Zhang
authors.forename: A B D
authors.initials: AB
authors.lastname: Mohamed
authors.forename: Kevin
authors.initials: K
authors.lastname: Fung
authors.forename: Keith
authors.initials: K
authors.lastname: Kwan
authors.forename: Tom W
authors.initials: TW
authors.lastname: Mara
authors.forename: Tom D
authors.initials: TD
authors.lastname: Daley
authors.forename: Eleftherios P
authors.initials: EP
authors.lastname: Diamandis
mesh_terms: D008949:Adenoma, Pleomorphic / Q000201:enzymology / Q000473:pathology; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D003528:Carcinoma, Adenoid Cystic / Q000201:enzymology / Q000473:pathology; D018277:Carcinoma, Mucoepidermoid / Q000201:enzymology / Q000473:pathology; D005557:Formaldehyde; D006801:Humans; D007150:Immunohistochemistry; D007610:Kallikreins / Q000096:biosynthesis*; D016612:Paraffin Embedding; D012189:Retrospective Studies; D012468:Salivary Gland Neoplasms / Q000201:enzymology* / Q000473:pathology; D012469:Salivary Glands / Q000201:enzymology* / Q000473:pathology; D016707:Tissue Fixation
chemical_list: D014408:Biomarkers, Tumor; D005557:Formaldehyde; C100936:KLK10 protein, human; D007610:Kallikreins