title: A novel method using an acedan-based Zn(DPA) probe to monitor ATP localization in an in vivo system.
abstract: In in vitro and in vivo systems, understanding localization and the functional role of ATP is essential, but effective methods to monitor ATP in cells and tissues are limited. Although quinacrine dihydrochloride is a well-known fluorescent dye used to detect ATP, it is limited in its use because it shows non-specific nuclear staining both in vitro and in vivo. A commercial luciferin-luciferase bioluminescence assay has also been used to detect ATP, but it can not be easily used in vivo. Thus, to effectively monitor ATP in vivo, we employed a novel two-photon ATP fluorescent probe, acedan-based Zn(DPA). Using the acedan-based Zn(DPA) probe, we show that this probe produces high quality images of ATP in lung, spleen, liver and spinal cord tissues.
authors.affiliation: Department of Anatomy, College of Medicine, Kyung Hee University, Heogi-Dong 1, Dongdaemun-Gu, Seoul 130-701, Republic of Korea.
authors.forename: Seo Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Alla Sreenivasa
authors.initials: AS
authors.lastname: Rao
authors.forename: Youn Ho
authors.initials: YH
authors.lastname: Shin
authors.forename: Hyung-Joo
authors.initials: HJ
authors.lastname: Chung
authors.forename: Youngbuhm
authors.initials: Y
authors.lastname: Huh
authors.forename: Kyo Han
authors.initials: KH
authors.lastname: Ahn
authors.forename: Junyang
authors.initials: J
authors.lastname: Jung
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D022781:Hepatocytes / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D011796:Quinacrine / Q000737:chemistry; D013116:Spinal Cord / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D015032:Zinc / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D000255:Adenosine Triphosphate; D011796:Quinacrine; D015032:Zinc