title: [Simultaneous determination of 14 restricted substances in flavor and fragrant by ultra high performance liquid chromatography].
abstract: An ultra high performance liquid chromatographic method was established for the separation and determination of 14 restricted substances in flavor and fragrant samples. The sample was extracted by 10% (v/v) methanol aqueous solution containing 1% (v/v) ammonia. The 14 analytes were separated in 12 min on a Waters BEH C18 column (50 mm x 2.1 mm, 1.7 microm) using acetonitrile and 10 mmol/L ammonium acetate aqueous solution (containing 0.1% (v/v) acetic acid) as mobile phases with gradient elution at a flow rate of 0.2 mL/min and 35 degrees C, and detected by a diode array detector scanned from 200 nm to 500 nm. The regression equations revealed acceptable linearity (correlation coefficients ranged from 0.995 0 to 0.999 9) in the range of 0.10 - 50 mg/L for the 14 analytes. The limits of detection (LODs) were from 0.32 mg/kg to 2.51 mg/kg. The recoveries of the 14 analytes spiked in real samples at 5, 10 and 20 mg/L were 93.0% - 121% with the relative standard deviations (RSDs) of 0.51% - 4.50%. With the advantages of accessibility, high sensitivity and good reproducibility, this simple method can be used in the simultaneous determination of the restricted substances in flavor and fragrant samples.
authors.affiliation: Yunnan Academy of Tobacco Science, Kunming 650106, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Jicang
authors.initials: J
authors.lastname: Xu
authors.forename: Xuemei
authors.initials: X
authors.lastname: Li
authors.forename: Jianguang
authors.initials: J
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
authors.forename: Mingming
authors.initials: M
authors.lastname: Miao
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009812:Odorants / Q000032:analysis*; D013649:Taste