title: Orosomucoid expression profiles in liver, adipose tissues and serum of lean and obese domestic pigs, Göttingen minipigs and Ossabaw minipigs.
abstract: The acute phase protein orosomucoid (ORM) has anti-inflammatory and immunomodulatory effects, and may play an important role in the maintenance of metabolic homeostasis in obesity-induced low-grade inflammation. Even though the pig is a widely used model for obesity related metabolic symptoms, the expression of ORM has not yet been characterized in such pig models. The objective of this study was to investigate the expression of ORM1 mRNA in liver, visceral adipose tissue, subcutaneous adipose tissue (SAT) from the abdomen or retroperitoneal abdominal adipose tissue (RPAT) and SAT from the neck, as well as the serum concentration of ORM protein in three porcine obesity models; the domestic pig, Göttingen minipigs and Ossabaw minipigs. No changes in ORM1 mRNA expression were observed in obese pigs compared to lean pigs in the four types of tissues. However, obese Ossabaw minipigs, but none of the other breeds, showed significantly elevated ORM serum concentrations compared to their lean counterparts. Studies in humans have shown that the expression of ORM was unchanged in adipose tissue depots in obese humans with an increased serum concentration of ORM. Thus in this respect, obese Ossabaw minipigs behave more similarly to obese humans than the other two pig breeds investigated.
authors.affiliation: Innate Immunology Group, National Veterinary Institute, Technical University of Denmark, Frederiksberg C, Denmark.
authors.forename: Tina
authors.initials: T
authors.lastname: Rødgaard
authors.forename: Jan
authors.initials: J
authors.lastname: Stagsted
authors.forename: Berit Ø
authors.initials: BØ
authors.lastname: Christoffersen
authors.forename: Susanna
authors.initials: S
authors.lastname: Cirera
authors.forename: Sophia G
authors.initials: SG
authors.lastname: Moesgaard
authors.forename: Michael
authors.initials: M
authors.lastname: Sturek
authors.forename: Mouhamad
authors.initials: M
authors.lastname: Alloosh
authors.forename: Peter M H
authors.initials: PM
authors.lastname: Heegaard
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D050152:Intra-Abdominal Fat / Q000378:metabolism; D008099:Liver / Q000276:immunology; D009765:Obesity / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D009961:Orosomucoid / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013045:Species Specificity; D050151:Subcutaneous Fat / Q000276:immunology; D034421:Sus scrofa / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D013552:Swine; D013556:Swine, Miniature / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D013851:Thinness / Q000097:blood / Q000235:genetics / Q000276:immunology; D014018:Tissue Distribution; D059467:Transcriptome
chemical_list: D009961:Orosomucoid; D012333:RNA, Messenger