title: Electrochemical immunosensor with N-doped graphene-modified electrode for label-free detection of the breast cancer biomarker CA 15-3.
abstract: Highly sensitive and label-free detection of the biomarker carbohydrate antigen 15-3 (CA 15-3) remains a challenge in the diagnosis of breast cancer. Here, a novel electrochemical immunosensor capable of sensitive and label-free detection of CA 15-3 is reported. This unique immunosensor, equipped with a highly conductive graphene (i.e., N-doped graphene sheets)-modified electrode, exhibited significantly increased electron transfer and high sensitivity toward CA 15-3. This novel immunosensor, with a low detection limit of 0.012 U/mL, worked well over a broad linear range of 0.1-20 U/mL. Unlike conventional immunosensors, which usually involve complicated label processing and time-consuming separations, the use of highly conductive graphene avoids the need for labels and is simple in nature. The strategy developed for this immunosensor provides a promising approach for clinical research and diagnostic applications.
authors.affiliation: School of Materials Science and Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: He
authors.initials: H
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: He
authors.forename: Songjun
authors.initials: S
authors.lastname: Li
authors.forename: Anthony P F
authors.initials: AP
authors.lastname: Turner
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D003217:Conductometry / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005260:Female; D006108:Graphite / Q000737:chemistry; D006801:Humans; D018396:Mucin-1 / Q000032:analysis*; D013194:Staining and Labeling
chemical_list: D014408:Biomarkers, Tumor; D018396:Mucin-1; D006108:Graphite