title: Dipeptides as leaving group in the enzyme-catalyzed DNA synthesis.
abstract: Conjugates of 2'-deoxyadenosine monophosphate with dipeptides have been synthesized and tested as substrates for several polymerases. Although the incorporation efficiency is not very high, it demonstrates that some of these dipeptides can be accommodated in the active site of polymerases and function as leaving groups in the enzymatic synthesis of DNA.
authors.affiliation: Laboratory of Medicinal Chemistry, Rega Institute for Medical Research, KU Leuven, Minderbroedersstraat 10, B-3000 Leuven.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Song
authors.forename: Camille
authors.initials: C
authors.lastname: Bouillon
authors.forename: Eveline
authors.initials: E
authors.lastname: Lescrinier
authors.forename: Piet
authors.initials: P
authors.lastname: Herdewijn
mesh_terms: D055162:Biocatalysis; D020134:Catalytic Domain; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003838:Deoxyadenine Nucleotides / Q000737:chemistry / Q000378:metabolism*; D004151:Dipeptides / Q000737:chemistry / Q000378:metabolism*; D054303:HIV Reverse Transcriptase / Q000378:metabolism
chemical_list: D003838:Deoxyadenine Nucleotides; D004151:Dipeptides; C012873:2'-deoxy-5'-adenosine monophosphate; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase; D004259:DNA-Directed DNA Polymerase