title: Role of the guanine nucleotide exchange factor Rom2 in cell wall integrity maintenance of Aspergillus fumigatus.
abstract: Aspergillus fumigatus is a mold and the causal agent of invasive aspergillosis, a systemic disease with high lethality. Recently, we identified and functionally characterized three stress sensors implicated in the cell wall integrity (CWI) signaling of this pathogen, namely, Wsc1, Wsc3, and MidA. Here, we functionally characterize Rom2, a guanine nucleotide exchange factor with essential function for the cell wall integrity of A. fumigatus. A conditional rom2 mutant has severe growth defects under repressive conditions and incorporates all phenotypes of the three cell wall integrity sensor mutants, e.g., the echinocandin sensitivity of the Δwsc1 mutant and the Congo red, calcofluor white, and heat sensitivity of the ΔmidA mutant. Rom2 interacts with Rho1 and shows a similar intracellular distribution focused at the hyphal tips. Our results place Rom2 between the cell surface stress sensors Wsc1, Wsc3, MidA, and Rho1 and their downstream effector mitogen-activated protein (MAP) kinase module Bck1-Mkk2-MpkA.
authors.affiliation: Max von Pettenkofer-Institut, Ludwig-Maximilians-Universität, Munich, Germany.
authors.forename: Sweta
authors.initials: S
authors.lastname: Samantaray
authors.forename: Michael
authors.initials: M
authors.lastname: Neubauer
authors.forename: Christoph
authors.initials: C
authors.lastname: Helmschrott
authors.forename: Johannes
authors.initials: J
authors.lastname: Wagener
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D001232:Aspergillus fumigatus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D000077336:Caspofungin; D002473:Cell Wall / Q000378:metabolism*; D025141:Drug Resistance, Fungal; D054714:Echinocandins / Q000494:pharmacology; D005656:Fungal Proteins / Q000502:physiology*; D055786:Gene Knockout Techniques; D020662:Guanine Nucleotide Exchange Factors / Q000502:physiology*; D025301:Hyphae / Q000254:growth & development / Q000378:metabolism; D055666:Lipopeptides; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010641:Phenotype; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D015398:Signal Transduction; D013172:Spores, Fungal / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D054714:Echinocandins; D005656:Fungal Proteins; D020662:Guanine Nucleotide Exchange Factors; D055666:Lipopeptides; D020928:Mitogen-Activated Protein Kinases; D020741:rho GTP-Binding Proteins; D000077336:Caspofungin