title: New bacteria Bacillus nitroreducens PLC9 with hydrogen peroxide-degrading activity with high survival rate in hydrogen peroxide.
abstract: Bacteria were isolated from wastewater containing highly concentrated hydrogen peroxide that had been used to clean the pure water delivery system in a semiconductor plant. One bacterium was selected for its high hydrogen peroxide degradation activity. In the presence of 1% hydrogen peroxide, it degraded 72.5% in 5 min. It showed 100% viability after 6 h at 1% hydrogen peroxide. Even at 3% hydrogen peroxide, it survived for more than 6 h. This bacterium was named as Bacillus nitroreducens PLC9 since its 16S rRNA showed 100% similarity with the recently reported new species B. nitroreducens. Purified catalase from B. nitroreducens PLC9 was characterized as a thermo-alkali-stable hydroperoxidase type II catalase, and it is suggested as a new type of catalase based on following: (1) it is stable over a broad pH range (pH 4-11); (2) it is consisted of homodimers with a molecular weight of 66 kDa (total molecular weight, 134 kDa); (3) its activity was not inhibited by 3-amino-1,2,4-triazole; and (4) its N-terminal sequence has never been reported before. Both B. nitroreducens PLC9 and the isolated catalase can be used for efficient degradation of hydrogen peroxide at high concentrations.
authors.affiliation: Culture Collection of Antimicrobial Resistant Microbes, Department of Biology, Seoul Women's University, Wharangro 623, Nowon-gu, Seoul 139-774, South Korea.
authors.forename: Hyunjin
authors.initials: H
authors.lastname: Hong
authors.forename: Kyung Sook
authors.initials: KS
authors.lastname: Bae
authors.forename: Yeonhee
authors.initials: Y
authors.lastname: Lee
mesh_terms: D000640:Amitrole / Q000494:pharmacology; D001407:Bacillus / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism* / Q000494:pharmacology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D062065:Wastewater / Q000382:microbiology
chemical_list: D000640:Amitrole; D001426:Bacterial Proteins; D002374:Catalase; D006861:Hydrogen Peroxide; D012336:RNA, Ribosomal, 16S; D062065:Wastewater