title: Structural, expression and interaction analysis of rice SKP1-like genes.
abstract: The degradation of proteins by the 26S proteasome is initiated by protein polyubiquitination mediated by a three-step cascade. The specific ubiquitination of different target proteins is mediated by different classes of E3 ubiquitin ligases, among which the best known are Skp1-Cullin-F-box complexes. Whereas protists, fungi and some vertebrates have a single SKP1 gene, many animal and plant species possess multiple SKP1 homologues. In this paper, we report on the structure, phylogeny and expression of the complete set of rice SKP1 genes (OSKs, Oryza sativa SKP1-like genes). Our analyses indicated that OSK1 and OSK20 belong to a class of SKP1 genes that contain one intron at a conserved position and are highly expressed. In addition, our yeast two-hybrid results revealed that OSK proteins display a differing ability to interact with F-box proteins. However, OSK1 and OSK20 seemed to interact with most of the nine F-box proteins tested. We suggest that rice OSK1 and OSK20 are likely to have functions similar to the Arabidopsis ASK1 and ASK2 genes.
authors.affiliation: Clermont Université, Université Blaise Pascal, UMR INRA-UBP 1095 GDEC, BP 10448, Clermont-Ferrand F-63000, France.
authors.forename: Senda
authors.initials: S
authors.lastname: Kahloul
authors.forename: Imen
authors.initials: I
authors.lastname: HajSalah El Beji
authors.forename: Aurélia
authors.initials: A
authors.lastname: Boulaflous
authors.forename: Ali
authors.initials: A
authors.lastname: Ferchichi
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Kong
authors.forename: Said
authors.initials: S
authors.lastname: Mouzeyar
authors.forename: Mohamed Fouad
authors.initials: MF
authors.lastname: Bouzidi
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D030541:Databases, Genetic; D044783:F-Box Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005810:Multigene Family; D012275:Oryza / Q000737:chemistry* / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification; D044786:S-Phase Kinase-Associated Proteins / Q000235:genetics* / Q000378:metabolism; D044843:SKP Cullin F-Box Protein Ligases / Q000235:genetics / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism
chemical_list: C120363:ASK1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D044783:F-Box Proteins; D010940:Plant Proteins; D018749:RNA, Plant; D044786:S-Phase Kinase-Associated Proteins; C484058:ASK2 protein, Arabidopsis; D044843:SKP Cullin F-Box Protein Ligases; D044767:Ubiquitin-Protein Ligases