title: Gliosis-specific transcription factor OASIS coincides with proteoglycan core protein genes in the glial scar and inhibits neurite outgrowth.
abstract: OASIS gene, a member of the CREB/ATF transcription factor family, is upregulated in gliosis after CNS injury. However it remains to be determined how OASIS is implicated in gliotic reaction. In a glial scar, chondroitin sulfate proteoglycans (CSPGs) are also upregulated, which engenders the inhibition of axonal regeneration. We investigated the functional role of OASIS in gliosis in relation to CSPG core proteins that render lesions non-permissive for regenerating axons. We first examined the gene expression localization of OASIS using several markers in a cryo-injured mouse brain and compared the expression pattern of CSPG core protein genes with that of OASIS in a glial scar by double-labeling in situ hybridization. Our findings suggest that OASIS is induced in proximal reactive astrocytes that exhibit upregulated expression for CSPGs, including NG2 proteoglycan, versican, brevican, neurocan, and phosphacan core. Furthermore, the membrane fraction derived from OASIS-transfected C6 cells inhibits neurite outgrowth of NG108-15 cells, whereas its neurite outgrowth inhibitory effect is abrogated after chondroitinase ABC treatment. OASIS is likely to be involved in the regulatory mechanism of non-permissive environments for axonal outgrowth.
authors.affiliation: Department of Emergency and Critical Care Medicine, Yamagata University School of Medicine, Iida-nishi, Yamagata, Japan. ken@med.id.yamagata-u.ac.jp
authors.forename: Ken
authors.initials: K
authors.lastname: Iseki
authors.forename: Seita
authors.initials: S
authors.lastname: Hagino
authors.forename: Takuya
authors.initials: T
authors.lastname: Nikaido
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Mori
authors.forename: Sachihiko
authors.initials: S
authors.lastname: Yokoya
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Hozumi
authors.forename: Kaoru
authors.initials: K
authors.lastname: Goto
authors.forename: Akio
authors.initials: A
authors.lastname: Wanaka
authors.forename: Choichiro
authors.initials: C
authors.lastname: Tase
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics / Q000378:metabolism*; D005911:Gliosis / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D016501:Neurites / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; C119923:Creb3l1 protein, mouse; D017362:Cyclic AMP Response Element-Binding Protein; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger