title: Detection of interleukin -1β from isolated human lymphocyte in response to lipopolysaccharide and lipoteichoic acid.
abstract: OBJECTIVE
  To detect the interleukin -1β levels from single and pooled isolated human lymphocytes in response to lipolysaccharide and lipoteichoic acid.
  
  
  METHODS
  Blood collected from healthy individuals (O +ve, A +ve, B +ve, and AB +ve) were subjected to gradient centrifugation to isolate lymphocytes. Different lymphocyte concentrations were used for in vitro pyrogen assay. Lymphocytes isolated were challenged with 5 EU of Gram negative (LPS) and 1 μg/μl of Gram positive (LTA) pyrogens in vitro and the inflammatory cytokine, Interleukin 1β (IL-1β) release was measured by Sandwich ELISA method.
  
  
  RESULTS
  The results indicated that the release of IL-1β increases immediately after the initiation of incubation and reaches a maximum at 4 to 6th hour and then stabilizes for both the pyrogens. Furthermore, IL-1β release by 5 EU of LPS and 1 μg/μl of LTA is dependent on lymphocytes concentration. It was also observed that the difference in blood group did not interfere with the IL-1β release.
  
  
  CONCLUSIONS
  The isolated lymphocyte system can be used as an alternative to the in vivo rabbit pyrogen assay.
authors.affiliation: Toxicology Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India.
authors.forename: Niveditha
authors.initials: N
authors.lastname: Lekshmi
authors.forename: Chandrika S
authors.initials: CS
authors.lastname: Geetha
authors.forename: Parayanthala V
authors.initials: PV
authors.lastname: Mohanan
mesh_terms: D023401:Animal Use Alternatives; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D053583:Interleukin-1beta / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D011749:Pyrogens / Q000494:pharmacology*; D013682:Teichoic Acids / Q000494:pharmacology*
chemical_list: D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D011749:Pyrogens; D013682:Teichoic Acids; C009900:lipoteichoic acid