title: New star-shaped carriers composed of β-cyclodextrin cores and disulfide-linked poly(glycidyl methacrylate) derivative arms with plentiful flanking secondary amine and hydroxyl groups for highly efficient gene delivery.
abstract: The biocleavable star-shaped vectors (CD-SS-PGEAs) consisting of nonionic β-cyclodextrin (β-CD) cores and disulfide-linked low-molecular-weight poly(glycidyl methacrylate) (PGMA) derivative arms with plentiful flanking secondary amine and hydroxyl groups were successfully proposed for highly efficient gene delivery. A simple two-step method was first adopted to introduce reduction-sensitive disulfide-linked initiation sites of atom transfer radical polymerization (ATRP) onto β-CD cores. The disulfide-linked PGMA arms prepared subsequently via ATRP were functionalized via the ring-opening reaction with ethanolamine (EA) to produce the cationic EA-functionalized PGMA (PGEA) arms with plentiful secondary amine and nonionic hydroxyl units. The cationic PGEA arms can be readily cleavable from the β-CD cores under reducible conditions. Such biocleavable star-shaped CD-SS-PGEA vectors possessed the good pDNA condensation ability, low cytotoxicity, and efficient gene delivery ability.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, College of Materials Science & Engineering, Beijing University of Chemical Technology, Beijing 100029 China.
authors.forename: Y
authors.initials: Y
authors.lastname: Hu
authors.forename: Y
authors.initials: Y
authors.lastname: Zhu
authors.forename: W T
authors.initials: WT
authors.lastname: Yang
authors.forename: F J
authors.initials: FJ
authors.lastname: Xu
mesh_terms: D000588:Amines / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry*; D018014:Gene Transfer Techniques / Q000295:instrumentation*; D056945:Hep G2 Cells; D006801:Humans; D015394:Molecular Structure; D010957:Plasmids / Q000235:genetics; D011109:Polymethacrylic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D014162:Transfection; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000588:Amines; D004220:Disulfides; D011109:Polymethacrylic Acids; D047392:beta-Cyclodextrins; C042535:polyglycidyl methacrylate