title: MiR-143 is not essential for adipose development as revealed by in vivo antisense targeting.
abstract: MiR-143 plays an important role in promoting the adipogenic differentiation of pre-adipocytes. Here, we report that systematic silencing of miR-143 in mice by using a locked-nucleic-acid-modified oligonucleotide (LNA-antimiR) did not lead to any obvious abnormalities in the adipose tissue differentiation. Furthermore, there were no significant differences in the expression level of several adipogenic marker genes, such as PPARγ and C/EBPα, in these animals compared with the controls. Therefore, we hypothesize that miR-143 may function as a fine tuning molecule rather than as a switch in the adipogenic regulatory network in mice. In addition, the proposed miR-143 target, ERK5, which was previously identified in human preadipocytes, was not effectively inhibited by miR-143 either in the murine preadipocyte cell line, 3T3-L1, or in primary mouse adipose tissue. However, we did fibroblast growth factor 7 (Fgf7) was identified as a target of miR-143 in murine adipogenesis.
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510006, People's Republic of China.
authors.forename: Zuyong
authors.initials: Z
authors.lastname: He
authors.forename: Jingwei
authors.initials: J
authors.lastname: Yu
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhou
authors.forename: Guangcai
authors.initials: G
authors.lastname: Ren
authors.forename: Peiqing
authors.initials: P
authors.lastname: Cong
authors.forename: Delin
authors.initials: D
authors.lastname: Mo
authors.forename: Yaosheng
authors.initials: Y
authors.lastname: Chen
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Liu
mesh_terms: D050156:Adipogenesis / Q000235:genetics / Q000502:physiology*; D000818:Animals; D002460:Cell Line; D051523:Fibroblast Growth Factor 7 / Q000037:antagonists & inhibitors*; D005786:Gene Expression Regulation; D020868:Gene Silencing; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*
chemical_list: C496454:Fgf7 protein, mouse; D035683:MicroRNAs; C532884:MIRN143 microRNA, mouse; D051523:Fibroblast Growth Factor 7