title: Pressure-overload hypertrophy of the developing heart reveals activation of divergent gene and protein pathways in the left and right ventricular myocardium.
abstract: Right ventricular (RV) and left ventricular (LV) myocardium differ in their pathophysiological response to pressure-overload hypertrophy. In this report we use microarray and proteomic analyses to identify pathways modulated by LV-aortic banding (AOB) and RV-pulmonary artery banding (PAB) in the immature heart. Newborn New Zealand White rabbits underwent banding of the descending thoracic aorta [LV-AOB; n = 6]. RV-PAB was achieved by banding the pulmonary artery (n = 6). Controls (n = 6 each) were sham-manipulated. After 4 (LV-AOB) and 6 (RV-PAB) wk recovery, the hearts were removed and matched RNA and proteins samples were isolated for microarray and proteomic analysis. Microarray and proteomic data demonstrate that in LV-AOB there is increased transcript expression levels for oxidative phosphorylation, mitochondria energy pathways, actin, ILK, hypoxia, calcium, and protein kinase-A signaling and increased protein expression levels of proteins for cellular macromolecular complex assembly and oxidative phosphorylation. In RV-PAB there is also an increased transcript expression levels for cardiac oxidative phosphorylation but increased protein expression levels for structural constituents of muscle, cardiac muscle tissue development, and calcium handling. These results identify divergent transcript and protein expression profiles in LV-AOB and RV-PAB and provide new insight into the biological basis of ventricular specific hypertrophy. The identification of these pathways should allow for the development of specific therapeutic interventions for targeted treatment and amelioration of LV-AOB and RV-PAB to ameliorate morbidity and mortality.
authors.affiliation: Division of Cardiothoracic Surgery, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.
authors.forename: Ingeborg
authors.initials: I
authors.lastname: Friehs
authors.forename: Douglas B
authors.initials: DB
authors.lastname: Cowan
authors.forename: Yeong-Hoon
authors.initials: YH
authors.lastname: Choi
authors.forename: Kendra M
authors.initials: KM
authors.lastname: Black
authors.forename: Reanne
authors.initials: R
authors.lastname: Barnett
authors.forename: Manoj K
authors.initials: MK
authors.lastname: Bhasin
authors.forename: Christian
authors.initials: C
authors.lastname: Daly
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Dillon
authors.forename: Towia A
authors.initials: TA
authors.lastname: Libermann
authors.forename: Francis X
authors.initials: FX
authors.lastname: McGowan
authors.forename: Pedro J
authors.initials: PJ
authors.lastname: del Nido
authors.forename: Sidney
authors.initials: S
authors.lastname: Levitsky
authors.forename: James D
authors.initials: JD
authors.lastname: McCully
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001013:Aorta, Thoracic / Q000503:physiopathology; D004195:Disease Models, Animal; D006352:Heart Ventricles / Q000378:metabolism; D017379:Hypertrophy, Left Ventricular / Q000235:genetics* / Q000378:metabolism* / Q000503:physiopathology; D017380:Hypertrophy, Right Ventricular / Q000235:genetics* / Q000378:metabolism* / Q000503:physiopathology; D008026:Ligation; D009206:Myocardium / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D040901:Proteomics; D011817:Rabbits; D059467:Transcriptome; D017725:Ventricular Pressure / Q000502:physiology