title: Molecular characterization and expression analysis of a novel cystatin-like gene in a hypoxia-tolerant Indian catfish, Clarias batrachus [Linnaeus, 1758].
abstract: A novel member of Cystatin superfamily was identified from Indian catfish, Clarias batrachus, in response to oxidation stress induced by environmental hypoxia. Integrated genomic approaches, expression profiling and computational techniques showed that CbCystatin had putative cystatin/monelin like domain and might be a transmembrane and/or intermediate protein in signaling pathways. CbCystatin was found to be clustered into family 2 Cystatins. At transcriptional level, its expression was significantly up-regulated in response to short as well as long periods (more than 20 fold) of hypoxia, suggesting its positive association with oxygen concentrations lower than physiological concentrations.
authors.affiliation: National Bureau of Fish Genetic Resources (ICAR), Lucknow, UP, India. vindhyamohindra@gmail.com
authors.forename: Vindhya
authors.initials: V
authors.lastname: Mohindra
authors.forename: Ratnesh K
authors.initials: RK
authors.lastname: Tripathi
authors.forename: Akanksha
authors.initials: A
authors.lastname: Singh
authors.forename: Balvinder
authors.initials: B
authors.lastname: Singh
mesh_terms: D000220:Adaptation, Biological / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002397:Catfishes / Q000235:genetics* / Q000502:physiology; D016000:Cluster Analysis; D019295:Computational Biology; D015891:Cystatins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000235:genetics; D023281:Genomics / Q000379:methods; D000860:Hypoxia / Q000235:genetics*; D007194:India; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D015398:Signal Transduction / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D015891:Cystatins; D017931:DNA Primers