title: Effects of β-hydroxybutyrate and isoproterenol on lipolysis in isolated adipocytes from periparturient dairy cows and cows with clinical ketosis.
abstract: An in vitro model was used to investigate effects of β-hydroxybutyrate and isoproterenol (β-adrenergic receptor agonist) on lipolysis in isolated adipocytes from late pregnant and recently calved dairy cows (n=5) and cows with clinical ketosis (n=3). Incubation with 3.0 mmol/L β-hydroxybutyrate reduced lipolysis in isolated adipocytes. This inhibitory effect was lower in the first lactation week (47%±16%) compared with late pregnancy (71%±6.5%). Incubation with 0.3 μmol/L isoproterenol stimulated lipolysis in isolated adipocytes from periparturient dairy cows. Basal lipolysis resulted in non-esterified fatty acid to glycerol ratios in the incubation media of 2.0±0.23 in prepartum samples, 2.1±0.23 in the first lactation week and 2.2±0.09 in cows with clinical ketosis. β-Hydroxybutyrate reduced lipolysis by 45%±9.6% in isolated adipocytes from cows with clinical ketosis, indicating that impaired feedback of β-hydroxybutyrate may not play a role in the disease etiology.
authors.affiliation: Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80151, 3508 TD Utrecht, The Netherlands.
authors.forename: S G A
authors.initials: SG
authors.lastname: van der Drift
authors.forename: R R
authors.initials: RR
authors.lastname: Everts
authors.forename: M
authors.initials: M
authors.lastname: Houweling
authors.forename: L A M G
authors.initials: LA
authors.lastname: van Leengoed
authors.forename: J A
authors.initials: JA
authors.lastname: Stegeman
authors.forename: A G M
authors.initials: AG
authors.lastname: Tielens
authors.forename: R
authors.initials: R
authors.lastname: Jorritsma
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000494:pharmacology*; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005260:Female; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology*; D007662:Ketosis / Q000378:metabolism / Q000662:veterinary*; D008066:Lipolysis / Q000187:drug effects*; D058725:Peripartum Period / Q000378:metabolism; D011247:Pregnancy
chemical_list: D007545:Isoproterenol; D020155:3-Hydroxybutyric Acid