title: Polymerase chain reaction detection of human host preference and Plasmodium parasite infections in field collected potential malaria vectors.
abstract: This study was carried out to determine the human host preference and presence of Plasmodium parasite in field collected Anopheles mosquitoes among four villages around a military cantonment located in malaria endemic Sonitpur district of Assam, India. Encountered malaria  vector mosquitoes were identified and tested for host preference and Plasmodium presence using PCR method. Human host preference was detected using simple PCR, whereas vectorial status for Plasmodium parasite was confirmed using first round PCR with genus specific primers and  thereafter nested PCR with three Plasmodium species specific primers. Out of 1874 blood fed vector mosquitoes collected, 187 (10%) were processed for PCR, which revealed that 40·6% had fed on human blood; 9·2% of human blood fed mosquito were harbouring Plasmodium  parasites, 71·4% of which were confirmed to Plasmodium falciparum. In addition to An. minimus, An. annularis and An. culicifacies were also found positive for malaria parasites. The present study exhibits the human feeding tendency of Anopheles vectors  highlighting their malaria parasite transmission potential. The present study may serve as a model for understanding the human host preference of malaria vectors and detection of malaria parasite inside the anopheline vector mosquitoes in order to update their vectorial status for estimating  the possible role of these mosquitoes in malaria transmission. The study has used PCR method and suggests that PCR-based method should be used in this entire malarious region to correctly report the vectorial position of different malaria vectors.
authors.affiliation: Defence Research Laboratory, Tezpur, India.
authors.forename: Sunil
authors.initials: S
authors.lastname: Dhiman
authors.forename: Rakesh Kumar
authors.initials: RK
authors.lastname: Bhola
authors.forename: Diganta
authors.initials: D
authors.lastname: Goswami
authors.forename: Bipul
authors.initials: B
authors.lastname: Rabha
authors.forename: Dinesh
authors.initials: D
authors.lastname: Kumar
authors.forename: Indra
authors.initials: I
authors.lastname: Baruah
authors.forename: Lokendra
authors.initials: L
authors.lastname: Singh
mesh_terms: D000818:Animals; D000852:Anopheles / Q000145:classification / Q000235:genetics / Q000469:parasitology* / Q000502:physiology*; D004199:Disease Vectors; D004772:Entomology / Q000379:methods; D005247:Feeding Behavior; D006801:Humans; D007194:India; D010274:Parasitology / Q000379:methods; D010961:Plasmodium / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D012424:Rural Population