title: Delivery of chemotherapeutic drugs in tumour cell-derived microparticles.
abstract: Cellular microparticles are vesicular plasma membrane fragments with a diameter of 100-1,000 nanometres that are shed by cells in response to various physiological and artificial stimuli. Here we demonstrate that tumour cell-derived microparticles can be used as vectors to deliver chemotherapeutic drugs. We show that tumour cells incubated with chemotherapeutic drugs package these drugs into microparticles, which can be collected and used to effectively kill tumour cells in murine tumour models without typical side effects. We describe several mechanisms involved in this process, including uptake of drug-containing microparticles by tumour cells, synthesis of additional drug-packaging microparticles by these cells that contribute to the cytotoxic effect and the inhibition of drug efflux from tumour cells. This study highlights a novel drug delivery strategy with potential clinical application.
authors.affiliation: Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Tang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Huafeng
authors.initials: H
authors.lastname: Zhang
authors.forename: Pingwei
authors.initials: P
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Jingwei
authors.initials: J
authors.lastname: Ma
authors.forename: Meng
authors.initials: M
authors.lastname: Lv
authors.forename: Dapeng
authors.initials: D
authors.lastname: Li
authors.forename: Foad
authors.initials: F
authors.lastname: Katirai
authors.forename: Guan-Xin
authors.initials: GX
authors.lastname: Shen
authors.forename: Guimei
authors.initials: G
authors.lastname: Zhang
authors.forename: Zuo-Hua
authors.initials: ZH
authors.lastname: Feng
authors.forename: Duyun
authors.initials: D
authors.lastname: Ye
authors.forename: Bo
authors.initials: B
authors.lastname: Huang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D002945:Cisplatin / Q000008:administration & dosage / Q000627:therapeutic use; D004337:Drug Carriers / Q000378:metabolism*; D005260:Female; D008113:Liver Neoplasms / Q000188:drug therapy; D008727:Methotrexate / Q000008:administration & dosage / Q000627:therapeutic use; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D009369:Neoplasms / Q000188:drug therapy*; D009374:Neoplasms, Experimental; D010051:Ovarian Neoplasms / Q000188:drug therapy
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D002945:Cisplatin; D008727:Methotrexate