title: [Preparation and preliminary characterization of chitosan from Catharsius molossus discards].
abstract: OBJECTIVE
  To study preparation and characterization of chitosan from Catharsius Molossus processing discards and explore the feasibility of utilization of insects residue.
  
  
  METHODS
  Preparation technique of chitosan was studied by orthogonal design,and properties were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), etc.
  
  
  RESULTS
  Preparation techniques were as follows: demineralizing: soaked for 30 min at 80 degrees C with 1.3 mol/L HCl, then kept for 12 h under room temperature. Deproteinization and delipidation: treated for 6 h at 90 degrees C with 4 mol/L NaOH. Decolorizing: soaked at room temperature with 3% KM-nO4, then treated with 2% oxalic acid at 70 degrees C. Deacetylating:treated for 5 h at 110 degrees C with 14 mol/L NaOH.
  
  
  CONCLUSIONS
  The technique is stable and feasible. The result also preliminarily showes that chitosan from Catharsius Molossus is better than shrimp's. It will be widely applied in biomedical and other industrial areas with such exciting properties.
authors.affiliation: Experimental Center of Biological Sciences, Southwest University of Science and Technology, Mianyang 621010, China. jiahuama@163.com
authors.forename: Jia-Hua
authors.initials: JH
authors.lastname: Ma
authors.forename: Cheng-Jia
authors.initials: CJ
authors.lastname: Tan
authors.forename: Xia
authors.initials: X
authors.lastname: Deng
authors.forename: Chao
authors.initials: C
authors.lastname: Xin
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002626:Chemistry, Pharmaceutical; D002686:Chitin / Q000032:analysis / Q000737:chemistry; D048271:Chitosan / Q000032:analysis / Q000737:chemistry*; D001517:Coleoptera / Q000737:chemistry*; D008419:Materia Medica / Q000737:chemistry; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D014866:Waste Products / Q000032:analysis*; D014961:X-Ray Diffraction
chemical_list: D008419:Materia Medica; D014866:Waste Products; D002686:Chitin; D048271:Chitosan