title: Simultaneous determination of 3-monochloropropane-1,2-diol and acrylamide in food by gas chromatography-triple quadrupole mass spectrometry with coupled column separation.
abstract: Both 3-monochloropropane-1,2-diol (3-MCPD) and acrylamide are contaminants found in heat-processed foods and their related products. A quantitative method was developed for the simultaneous determination of both contaminants in food by gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS). The analytes were purified and extracted by the matrix solid-phase dispersion extraction (MSPDE) technique with Extrelut NT. A coupled column (a 3 m Innowax combined with a 30 m DB-5 ms) was developed to separate both compounds efficiently without derivatization. Triple quadrupole mass spectrometry in multiple reaction monitoring mode (MRM) was applied to suppress matrix interference and obtain good sensitivity in the determination of both analytes. The limit of detection (LOD) in the sample matrix was 5 μg kg(-1) for 3-MCPD or acrylamide. The average recoveries for 3-MCPD and acrylamide in different food matrices were 90.5-107% and 81.9-95.7%, respectively, with the intraday relative standard deviations (RSDs) of 5.6-13.5% and 5.3-13.4%, respectively. The interday RSDs were 6.1-12.6% for 3-MCPD and were 5.0-12.8% for acrylamide. Both contaminants were found in samples of bread, fried chips, fried instant noodles, soy sauce, and instant noodle flavoring. Neither 3-MCPD nor acrylamide was detected in the samples of dairy products (solid or liquid samples) and non-fried instant noodles.
authors.affiliation: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
authors.forename: Xiao-min
authors.initials: XM
authors.lastname: Xu
authors.forename: Hua-li
authors.initials: HL
authors.lastname: He
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
authors.forename: Liang
authors.initials: L
authors.lastname: Feng
authors.forename: Ying
authors.initials: Y
authors.lastname: Ying
authors.forename: Bai-fen
authors.initials: BF
authors.lastname: Huang
authors.forename: Hai-tao
authors.initials: HT
authors.lastname: Shen
authors.forename: Jian-long
authors.initials: JL
authors.lastname: Han
authors.forename: Yi-ping
authors.initials: YP
authors.lastname: Ren
mesh_terms: D020106:Acrylamide / Q000032:analysis* / Q000302:isolation & purification; D000818:Animals; D002417:Cattle; D003611:Dairy Products / Q000032:analysis; D057140:Fast Foods / Q000032:analysis; D005421:Flavoring Agents / Q000032:analysis; D005506:Food Contamination / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D005990:Glycerol / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D008892:Milk / Q000737:chemistry; D045730:Soy Foods / Q000032:analysis; D000517:alpha-Chlorohydrin
chemical_list: D005421:Flavoring Agents; D020106:Acrylamide; D000517:alpha-Chlorohydrin; D005990:Glycerol