title: Identification of a fatty acid ∆(6)-desaturase gene from the eicosapentaenoic acid-producing fungus Pythium splendens RBB-5.
abstract: A new full-length cDNA (PsD6) putatively encoding a ∆(6)-desaturase was cloned from the eicosapentaenoic acid-producing fungus Pythium splendens RBB-5. PsD6 contained an open reading frame of 1380 bp encoding a protein of 459 amino acids. The deduced amino acid sequence showed high similarity to those of other ∆(6)-desaturases. A recombinant vector expressing PsD6 (pPIC3.5K-D6) was constructed and transformed into Pichia pastoris GS115. The heterologous expressed PsD6 in P. pastoris desaturated linoleic acid to γ-linolenic acid but not desaturated α-linolenic acid to stearidonic acid. The results indicated that PsD6 was a fatty acid ∆(6)-desaturase and it had a substrate specificity for linoleic acid.
authors.affiliation: Department of Biotechnology, Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Ruijiao
authors.initials: R
authors.lastname: Zhang
authors.forename: Yuanmin
authors.initials: Y
authors.lastname: Zhu
authors.forename: Liang
authors.initials: L
authors.lastname: Ren
authors.forename: Pengpeng
authors.initials: P
authors.lastname: Zhou
authors.forename: Jingrong
authors.initials: J
authors.lastname: Hu
authors.forename: Longjiang
authors.initials: L
authors.lastname: Yu
mesh_terms: D001711:Biotransformation; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D015118:Eicosapentaenoic Acid / Q000378:metabolism*; D015870:Gene Expression; D005822:Genetic Vectors; D008041:Linoleic Acids / Q000378:metabolism; D050602:Linoleoyl-CoA Desaturase / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010843:Pichia / Q000235:genetics; D011775:Pythium / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D004271:DNA, Fungal; D008041:Linoleic Acids; D015118:Eicosapentaenoic Acid; D050602:Linoleoyl-CoA Desaturase