title: Genetic variability of PRNP in Chinese indigenous goats.
abstract: Polymorphism of the prion protein gene (PRNP) is usually associated with scrapie susceptibility or resistance. To determine the variability of PRNP in Chinese indigenous goat breeds, we isolated genomic DNA from goat blood and amplified and sequenced the coding region of the gene. We identified 10 polymorphic sites that gave rise to 28 haplotypes. Clear frequency differences were found between northern and southern breeds and confirmed by genetic distance analysis, except for the Tangshan dairy goat. Phylogeographic analysis supported the idea that northern and southern breeds might be considered separate clusters, except for the Tangshan dairy goat. The finding of significant differences in allele distribution in northern and southern goats, especially if involved in modulating resistance/susceptibility, needs to be carefully considered for the feasibility of selection plans for resistance to scrapie.
authors.affiliation: College of Animal Science and Technology, Agricultural University of Hebei, 289 Lingyusi Street, Baoding, 071001, China. rongyanzhou@yahoo.com.cn
authors.forename: Rongyan
authors.initials: R
authors.lastname: Zhou
authors.forename: Xianglong
authors.initials: X
authors.lastname: Li
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Xi
authors.forename: Lanhui
authors.initials: L
authors.lastname: Li
authors.forename: Zhenhong
authors.initials: Z
authors.lastname: Zhang
authors.forename: Zhujun
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D000483:Alleles; D000818:Animals; D002681:China; D020022:Genetic Predisposition to Disease / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006041:Goats; D011110:Polymorphism, Genetic; D011328:Prions / Q000235:genetics*; D012608:Scrapie / Q000235:genetics
chemical_list: D011328:Prions