title: Preparation of carboxymethyl chitosan sulfate for improved cell proliferation of skin fibroblasts.
abstract: Carboxymethyl chitosan (CMC) was chemically modified by sulfating agent N(SO(3)Na)(3). The product carboxymethyl chitosan sulfate (CMCS) was characterized by Fourier transform infrared spectroscopy (FT-IR) and carbon-13 nuclear magnetic resonance spectroscopy ((13)C NMR). And the optimal reaction conditions were also studied by means of single factor experiment. MTT method was applied to evaluate the effects of CMCS on proliferation of skin fibroblasts. The results revealed the CMCS with sulfate content of 26.26% at the concentration of 100 μg/ml best promoted the proliferation of skin fibroblasts.
authors.affiliation: Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan 430070, China.
authors.forename: Shengxiang
authors.initials: S
authors.lastname: Tao
authors.forename: Song
authors.initials: S
authors.lastname: Gao
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhou
authors.forename: Mi
authors.initials: M
authors.lastname: Cao
authors.forename: Weiguo
authors.initials: W
authors.lastname: Xie
authors.forename: Hua
authors.initials: H
authors.lastname: Zheng
authors.forename: Lihong
authors.initials: L
authors.lastname: Fan
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D051381:Rats; D012867:Skin / Q000166:cytology*; D017550:Spectroscopy, Fourier Transform Infrared; D013431:Sulfates / Q000032:analysis; D013696:Temperature; D013997:Time Factors
chemical_list: D013431:Sulfates; C514968:carboxymethyl-chitosan; C480355:chitosan sulfate; D048271:Chitosan