title: Poly(triallyl isocyanurate-co-ethylene dimethacrylate-co-alkyl methacrylate) stationary phases in the chromatographic separation of hydrophilic solutes.
abstract: This study describes the ability of triallyl isocyanurate (TAIC)-co-methacrylate ester polymer monoliths as stationary phases for the separation of hydrophilic compounds (phenolic acids, amino acids and catecholamines) in capillary electrochromatography (CEC) and ultra high pressure liquid chromatography (UHPLC). Several TAIC-co-methacrylate ester polymer monoliths prepared by single-step in situ copolymerization of TAIC, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methylpropane sulfonic acid (AMPS), with or without alkyl methacrylates were characterized by examining the SEM image, surface area, contact angle, and the thermal decomposition temperature. Compared to the conventional methacrylate ester-based monoliths, these proposed monoliths possessed hydrophilic character thus increased wettability which improved chromatographic separation selectivity of polar phenolic acids. Among the proposed TAIC-co-methacrylate monoliths, poly(TAIC-co-EDMA-AMPS-co-stearyl methacrylate (SMA)) showed separation selectivity with an increased analyte resolution from 0.0 to 0.92 for 4-hydroxybenzoic acid and vanillic acid, which were consistently difficult to resolve in the reversed-phase chromatographic mechanism of these monoliths in aqueous mobile phases. Moreover, stable ionization efficiencies were observed when this monolith was combined with ESI-MS detector possibly because an organic solvent-rich sheath liquid was used in the CEC-MS. This study demonstrates the potentiality of novel TAIC-co-methacrylate polymer monoliths in hydrophilic solute separation either in CEC or UHPLC mode.
authors.affiliation: Department of Chemistry and Center for Nanotechnology at CYCU, Chung Yuan Christian University, Chung-Li 320, Taiwan.
authors.forename: Cheng-Lan
authors.initials: CL
authors.lastname: Lin
authors.forename: Brenda
authors.initials: B
authors.lastname: Singco
authors.forename: Ching-Yi
authors.initials: CY
authors.lastname: Wu
authors.forename: Pei-Zhu
authors.initials: PZ
authors.lastname: Liang
authors.forename: Yi-Jie
authors.initials: YJ
authors.lastname: Cheng
authors.forename: Hsi-Ya
authors.initials: HY
authors.lastname: Huang
mesh_terms: D000596:Amino Acids / Q000032:analysis; D053801:Capillary Electrochromatography / Q000379:methods*; D002395:Catecholamines / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D062385:Hydroxybenzoates / Q000302:isolation & purification; D008689:Methacrylates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D012997:Solvents; D013696:Temperature; D014227:Triazines / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D002395:Catecholamines; D062385:Hydroxybenzoates; D008689:Methacrylates; D012997:Solvents; D014227:Triazines; C480124:1,3,5-triallyl isocyanurate; C004919:ethylene dimethacrylate; C017616:phenolic acid