title: Chromosome-centric approach to overcoming bottlenecks in the Human Proteome Project.
abstract: The international Human Proteome Project (HPP), a logical continuation of the Human Genome Project, was launched on 23 September 2010 in Sydney, Australia. In accordance with the gene-centric approach, the goals of the HPP are to prepare an inventory of all human proteins and decipher the network of cellular protein interactions. The greater complexity of the proteome in comparison to the genome gives rise to three bottlenecks in the implementation of the HPP. The main bottleneck is the insufficient sensitivity of proteomic technologies, hampering the detection of proteins with low- and ultra-low copy numbers. The second bottleneck is related to poor reproducibility of proteomic methods and the lack of a so-called 'gold' standard. The last bottleneck is the dynamic nature of the proteome: its instability over time. The authors here discuss approaches to overcome these bottlenecks in order to improve the success of the HPP.
authors.affiliation: Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, 119121, Pogodinskaya Street 10, Moscow, Russia. inst@ibmc.msk.ru
authors.forename: Alexander
authors.initials: A
authors.lastname: Archakov
authors.forename: Victor
authors.initials: V
authors.lastname: Zgoda
authors.forename: Arthur
authors.initials: A
authors.lastname: Kopylov
authors.forename: Stanislav
authors.initials: S
authors.lastname: Naryzhny
authors.forename: Alexey
authors.initials: A
authors.lastname: Chernobrovkin
authors.forename: Elena
authors.initials: E
authors.lastname: Ponomarenko
authors.forename: Andrey
authors.initials: A
authors.lastname: Lisitsa
mesh_terms: D002877:Chromosomes, Human; D006801:Humans; D057230:Limit of Detection; D020543:Proteome; D013816:Thermodynamics
chemical_list: D020543:Proteome