title: Influence of environmental conditions on the expression and the maturation process of the Clostridium difficile surface associated protease Cwp84.
abstract: Expression of the Clostridium difficile protease gene, cwp84, was moderately up-regulated by decreasing pH due to glucose metabolism. Purification under different pH conditions influenced the proteolytic process of Cwp84. Given this, acidic pH could favor the appearance of different forms of Cwp84 that may have different roles during the infection.
authors.affiliation: EA 4043, Université Paris-Sud 11, Faculté de Pharmacie, 5 rue Jean Baptiste Clément, 92296 Châtenay-Malabry, France.
authors.forename: Diana
authors.initials: D
authors.lastname: Chapetón Montes
authors.forename: Anne
authors.initials: A
authors.lastname: Collignon
authors.forename: Claire
authors.initials: C
authors.lastname: Janoir
mesh_terms: D016360:Clostridioides difficile / Q000201:enzymology* / Q000235:genetics*; D003470:Culture Media / Q000737:chemistry; D003546:Cysteine Endopeptidases / Q000096:biosynthesis*; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D006863:Hydrogen-Ion Concentration
chemical_list: D003470:Culture Media; C524258:Cwp84 protein, Clostridium difficile; D003546:Cysteine Endopeptidases