title: Vaccination of mice with an antigenic serine protease-like protein elicits a protective immune response against Trichinella spiralis infection.
abstract: Trichinellosis has major economic impacts on animal husbandry and food safety, and the control and elimination of trichinellosis is a major objective of veterinary medicine. A gene encoding serine protease of Trichinella spiralis (Ts-Adsp) was identified by immunoscreening an adult T. spiralis cDNA library. In this study, the recombinant Ts-Adsp protein (rTs-Adsp) was cloned and expressed in a prokaryotic expression system and purified by Ni-affinity chromatography. To determine whether the purified rTs-Adsp is a potential vaccine candidate for the control of T. spiralis infection, we immunized BALB/c mice with this protein in combination with an alum adjuvant and subsequently challenged with T. spiralis larvae. The results showed that mice vaccinated with rTs-Adsp exhibited an average reduction in the muscle larvae burden of 46.5% relative to the control group. Immunization with the rTs-Adsp antigen induced both humoral and cellular immune responses, which manifested as elevated specific anti-rTs-Adsp IgG and IgE antibodies and a mixed Th1-Th2 response, as determined by Th1 (IFN-γ and IL-2) and Th2 (IL-4, IL-10, and IL-13) cytokine profiling, with the Th2 predominant. Thus, purified rTs-Adsp is able to limit the invasion of T. spiralis , and this protein could be an effective vaccine candidate for trichinellosis.
authors.affiliation: Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Feng
authors.forename: Xiuping
authors.initials: X
authors.lastname: Wu
authors.forename: Xuelin
authors.initials: X
authors.lastname: Wang
authors.forename: Xue
authors.initials: X
authors.lastname: Bai
authors.forename: Haining
authors.initials: H
authors.lastname: Shi
authors.forename: Bin
authors.initials: B
authors.lastname: Tang
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Liu
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Song
authors.forename: Pascal
authors.initials: P
authors.lastname: Boireau
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhao
authors.forename: Mingyuan
authors.initials: M
authors.lastname: Liu
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D016516:CD4-CD8 Ratio; D016207:Cytokines / Q000378:metabolism; D018076:DNA, Complementary / Q000008:administration & dosage / Q000276:immunology; D018509:DNA, Helminth / Q000008:administration & dosage / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology; D057057:Serine Proteases / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D017160:Trichinella spiralis / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology / Q000517:prevention & control*; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D016207:Cytokines; D018076:DNA, Complementary; D018509:DNA, Helminth; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D019444:Vaccines, DNA; D007073:Immunoglobulin E; D057057:Serine Proteases