title: Genetic deficiency in complement component 4b does not alter radiation-induced lung disease in mice.
abstract: Previous investigations have shown altered levels of complement components to be associated with radiation-induced lung disease. In this study we aimed to determine whether a deficiency in complement component 4b alters the lung response to irradiation of C57BL/6 mice. The pulmonary phenotype of C57BL/6 C4b(-/-) mice and their wild-type littermates was assessed following an 18 Gy single dose to the thoracic cavity. The assessed end points included, survival time postirradiation, bronchoalveolar lavage cell differential, hydroxyproline measures and histological evidence of alveolitis and fibrosis. The lung phenotype of C4b-deficient mice did not differ from that of wild-type mice in terms of survival time postirradiation, tissue hydroxyproline levels or by histological evidence of alveolitis or fibrosis. No differences in bronchoalveolar cell differential counts were evident among the irradiated mice grouped by C4b genotype. We concluded that a deficiency in C4b does not alter radiation-induced lung disease in the C57BL/6 mouse model.
authors.affiliation: Department of Medicine and the Meakins-Christie Laboratories, McGill University, 3626 St. Unbain Montreal, Quebec, Canada.
authors.forename: Jessica
authors.initials: J
authors.lastname: Fox
authors.forename: Marie-Eve
authors.initials: ME
authors.lastname: Bergeron
authors.forename: Christina K
authors.initials: CK
authors.lastname: Haston
mesh_terms: D000818:Animals; D001835:Body Weight / Q000235:genetics / Q000528:radiation effects; D002452:Cell Count; D015935:Complement C4b / Q000172:deficiency* / Q000235:genetics*; D005260:Female; D055786:Gene Knockout Techniques; D008171:Lung Diseases / Q000209:etiology* / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D010641:Phenotype; D011833:Radiation Injuries, Experimental / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015996:Survival Rate; D013909:Thorax / Q000528:radiation effects
chemical_list: D015935:Complement C4b