title: Synthetic glycolipids for glioma growth inhibition developed from neurostatin and NF115 compound.
abstract: Neurostatin, a natural glycosphingolipid, and NF115, a synthetic glycolipid, are inhibitors of glioma growth. While neurostatin shows high inhibitory activity on gliomas its abundance is low in mammalian brain. On the contrary NF115 exhibits less inhibitory activity on gliomas, but could be prepared by chemical synthesis. In this study we describe synthetic compounds, structurally related to NF115, capable of inhibiting glioma growth at low micromolar range. We used DNA microarray technology to compare the genes inhibited in U373-MG human glioma cells after treatment with the natural or synthetic inhibitor. New synthetic compounds were developed to interact with the product of Rho GDP dissociation inhibitor alpha gene, which was repressed in both treatments. Compounds that were inhibitors of glioma cell growth in assays for [3H]-thymidine incorporation were then injected in C6 tumor bearing rats and the tumor size in each animal group were measured. The GC-17, GC-4 and IG-5 are new compounds derived from NF115 and showed high antiproliferative activity on tumor cell lines. The GC-17 compound inhibited U373-MG glioblastoma cells (3.2 μM), the effects was fifty times more potent than NF115, and caused a significant reduction of tumor volume (P<0.05) when tested in Wistar rats allotransplanted with C6 glioma cells.
authors.affiliation: Grupo de Química Neuro-regenerativa, Unidad Neurología Experimental, Hospital Nacional de Parapléjicos, Servicio de Salud de Castilla La Mancha (SESCAM), Finca La Peraleda s/n, 45071 Toledo, Spain. ernestod@sescam.jccm.es
authors.forename: Ernesto
authors.initials: E
authors.lastname: Doncel-Pérez
authors.forename: Isabel
authors.initials: I
authors.lastname: García-Álvarez
authors.forename: Alfonso
authors.initials: A
authors.lastname: Fernández-Mayoralas
authors.forename: Manuel
authors.initials: M
authors.lastname: Nieto-Sampedro
mesh_terms: D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005786:Gene Expression Regulation; D005910:Glioma / Q000188:drug therapy*; D006017:Glycolipids / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D006028:Glycosphingolipids / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D046228:Microarray Analysis; D011409:Propylene Glycols / Q000737:chemistry* / Q000494:pharmacology; D062948:rho Guanine Nucleotide Dissociation Inhibitor alpha / Q000235:genetics / Q000378:metabolism
chemical_list: C473670:2-acetamido-2-deoxy-6-O-(2,2-bis(hydroxymethyl)-3-hydroxypropyl)glucopyranoside; D006017:Glycolipids; D006028:Glycosphingolipids; D011409:Propylene Glycols; C116059:neurostatin; D062948:rho Guanine Nucleotide Dissociation Inhibitor alpha; D000117:Acetylglucosamine