title: [Formula-syndrome correlation study of three classical anti-jaundice formulas in inhibition of liver fibrosis induced by dimethylnitrosamine in rats].
abstract: OBJECTIVE
  To investigate the effects of three classical anti-jaundice formulas Yinchenhao Decoction (YCHD). Yinchen Wuling San (YCWLS) and Zhizi Baipi Decoction (ZZBPD) on liver fibrosis induced by dimethylnitrosamine (DMN) in rats and explore the formula-syndrome relationship.
  
  
  METHODS
  Forty-eight rats were weighted, and divided into five groups: normal control, DMN, YCHD, YCWLS and ZZBPD groups using stratified random method. Liver fibrosis in rats was induced by intraperitoneal injection of DMN for 4 weeks at the dosage 10 mg/kg body weight, once per day for 3 consecutive days in each week. Each groups was gavaged with distilled water (control or model group), YCHD, YCWLS and ZZBPD respectively in the last 2-week modeling period. At the end of the 4th week, rats were sacrificed and their blood sample and liver tissue were taken for detecting pathology and analyzing of hydroxyproline (Hyp) and liver function.
  
  
  RESULTS
  Compared with the normal control group, DMN model group showed liver damage and liver fibrosis with remarkably increased Hyp content (P<0.01) and abnormal liver function (P<0.01). Liver fibrosis induced by DMN in rats was improved by administration of YCHD. Compared with DMN model group, content of Hyp was decreased remarkably (P<0.01) and liver function and hepatic histology were improved significantly after 2 weeks of YCHD treatment (P<0.05 or P<0.01)., and content of hepatic Hyp was decreased in the ZZBPD groups. Comparison of various parameters between groups showed that content of serum total bilirubin was decreased significantly in the YCWLS group (P<0.01), and content of hepatic Hyp was decreased in the ZZBPD group.
  
  
  CONCLUSIONS
  Efficacy of YCHD in inhibition of liver fibrosis induced by DMN in rats is far better than YCWLS and ZZBPD. The pathogenesis of the liver fibrosis induced by DMN is mainly damp-heat. In addition, both damp and heat pathogenic factors play very important role in this model. The syndrome is highly correlated with the prescription YCHD.
authors.affiliation: Institute of Liver Disease, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Yan-qin
authors.initials: YQ
authors.lastname: Bian
authors.forename: Bing-bing
authors.initials: BB
authors.lastname: Ning
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Cao
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.forename: Cheng
authors.initials: C
authors.lastname: Liu
authors.forename: Gao-feng
authors.initials: GF
authors.lastname: Chen
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Ming-yu
authors.initials: MY
authors.lastname: Sun
mesh_terms: D000818:Animals; D004128:Dimethylnitrosamine / Q000009:adverse effects*; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D006909:Hydroxyproline / Q000032:analysis; D008099:Liver / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced* / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D008517:Phytotherapy; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004365:Drugs, Chinese Herbal; D004128:Dimethylnitrosamine; D006909:Hydroxyproline