title: Quantification of cytomegalovirus glycoprotein Bn DNA in hematopoietic stem cell transplant recipients by real-time PCR.
abstract: Based on sequence variation in the N-terminus of the UL55 gene, which encodes glycoprotein B (gB), human cytomegalovirus (CMV) can be classified into four gBn genotypes. We assessed the distribution of CMV gBn genotypes and the correlation between CMV gBn DNA (detected by real-time PCR) and CMV-positive pp65 cells (identified by immunohistochemical staining) in a cohort of hematopoietic stem cell transplant patients. The distribution of gB genotypes was as follows: gBn1, 60% of patients; gBn2, 13.3%; mixed gBn1 and gBn3 infection, 26.7%; and gBn4 and other mixed infections, 0%. CMV gBn1 was the most common genotype. The detected level of CMV gB DNA correlated well with the number of CMV-positive pp65 cells detected by immunostaining (r = 0.585).
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhang
authors.forename: Ya Ping
authors.initials: YP
authors.lastname: Huang
authors.forename: Hai Nv
authors.initials: HN
authors.lastname: Gao
authors.forename: Mei Fang
authors.initials: MF
authors.lastname: Yang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhao
authors.forename: Jian Hua
authors.initials: JH
authors.lastname: Hu
authors.forename: Xiao Ming
authors.initials: XM
authors.lastname: Chen
authors.forename: Wei Hang
authors.initials: WH
authors.lastname: Ma
authors.forename: Jun
authors.initials: J
authors.lastname: Fan
mesh_terms: D000293:Adolescent; D000328:Adult; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D006023:Glycoproteins / Q000235:genetics*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008297:Male; D060888:Real-Time Polymerase Chain Reaction; D014764:Viral Proteins / Q000235:genetics*; D055815:Young Adult
chemical_list: D006023:Glycoproteins; D014764:Viral Proteins