title: Assessment of DFG-S19 method for the determination of common endocrine disruptor pesticides in wine samples with an estimation of the uncertainty of the analytical results.
abstract: A gas chromatographic method for the determination of endocrine disruptor pesticides (Chlorpyrifos, Penconazole, Procymidone, Iprodione, Bromopropylate and Lambda-Cyhalothrin) in wine samples is described. A general DFG-S19 method for residual pesticide determination in all kind of food stuff was investigated to simplify and adopt for wine samples in this work. Alternative sample preparation routes were elucidated and compared according to their recovery values. Four different separation techniques were tested and the method employing florosil column after a LLE procedure was applied for wine samples with satisfactory recovery ratios (72-97%). The pesticides were extracted from the sample by cyclohexane-ethyl acetate mixture (1:1 v/v) and cleaned up by florosil column. The regression coefficients were at least 0.99 and relative standard deviations were no higher than 16%. Detection limits were in the range of 0.6-2.9 ng/mL and the relative expanded measurement uncertainties were calculated in the 7-22% range.
authors.affiliation: Ege University, Science Faculty, Department of Chemistry, 35100 Bornova, İzmir, Turkey. fusun.okcu@ege.edu.tr
authors.forename: Füsun Okçu
authors.initials: FO
authors.lastname: Pelit
authors.forename: Hasan
authors.initials: H
authors.lastname: Ertaş
authors.forename: Işıl
authors.initials: I
authors.lastname: Seyrani
authors.forename: F Nil
authors.initials: FN
authors.lastname: Ertaş
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D052244:Endocrine Disruptors / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D057230:Limit of Detection; D010573:Pesticide Residues / Q000032:analysis*; D014920:Wine / Q000032:analysis*
chemical_list: D052244:Endocrine Disruptors; D010573:Pesticide Residues