title: Merkel cell polyomavirus infection in both components of a combined Merkel cell carcinoma and basal cell carcinoma with ductal differentiation; each component had a similar but different novel Merkel cell polyomavirus large T antigen truncating mutation.
abstract: Merkel cell polyomavirus infects up to 80% of patients with Merkel cell carcinoma. Combined Merkel cell carcinoma and cutaneous tumors occur occasionally. Previous reports have suggested that Merkel cell polyomavirus is absent from combined Merkel cell carcinoma and squamous cell carcinomas. This is the first report that Merkel cell polyomavirus infected in both lesions of a combined Merkel cell carcinoma and basal cell carcinoma. A 92-year-old Japanese man presented with a right thigh small subcutaneous mass. Histologic examination revealed a combined tumor with Merkel cell carcinoma and basal cell carcinoma with ductal differentiation. Both tumors and intermingled Merkel cells in basal cell carcinoma expressed Merkel cell polyomavirus large T antigen, and 17 and 240 copies of Merkel cell polyomavirus/cell were detected in the microdissected Merkel cell carcinoma and basal cell carcinoma specimens, respectively. Mutation analysis of Merkel cell polyomavirus large T antigen revealed a novel truncating mutation in Merkel cell carcinoma and a similar but different mutation in the basal cell carcinoma. These results suggest that each was infected by a different Merkel cell polyomavirus subclone derived from a single Merkel cell polyomavirus.
authors.affiliation: Division of Molecular Pathology, Department of Pathology, Tottori University Faculty of Medicine, Nishi-cho 86, Yonago, Tottori, 683-8503, Japan. iwasakit-path@umin.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Iwasaki
authors.forename: Hajime
authors.initials: H
authors.lastname: Kodama
authors.forename: Michiko
authors.initials: M
authors.lastname: Matsushita
authors.forename: Naoto
authors.initials: N
authors.lastname: Kuroda
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Yamasaki
authors.forename: Ichiro
authors.initials: I
authors.lastname: Murakami
authors.forename: Osamu
authors.initials: O
authors.lastname: Yamamoto
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Hayashi
mesh_terms: D000369:Aged, 80 and over; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics* / Q000378:metabolism; D002280:Basal Cell Carcinoma / Q000235:genetics / Q000473:pathology / Q000821:virology*; D015266:Carcinoma, Merkel Cell / Q000235:genetics / Q000473:pathology / Q000821:virology*; D004252:DNA Mutational Analysis; D004279:DNA, Viral / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D059965:Merkel cell polyomavirus / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D009154:Mutation; D009378:Neoplasms, Multiple Primary / Q000235:genetics / Q000473:pathology / Q000821:virology*; D016133:Polymerase Chain Reaction; D027601:Polyomavirus Infections / Q000235:genetics / Q000473:pathology / Q000821:virology; D012878:Skin Neoplasms / Q000235:genetics / Q000473:pathology / Q000821:virology*; D014412:Tumor Virus Infections / Q000235:genetics / Q000473:pathology / Q000821:virology; D019562:Viral Load
chemical_list: D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral