title: Increased expression of CD24 in nonmelanoma skin cancer.
abstract: BACKGROUND
  Skin cancer detection is based on the macroscopic and microscopic appearance of the lesions and the experience of the surgeon. The final diagnosis is done by pathological analysis, based on established criteria. Currently, there is no serum marker that can be used for the diagnosis of skin cancer. CD24, a mucin-like glycoprotein, is overexpressed in a variety of cancers including skin malignancies.
  
  
  OBJECTIVE
  Evaluate the potential utility of CD24 expression in peripheral blood leukocytes (PBLs) for the detection of nonmelanoma skin cancers (NMSC).
  
  
  METHODS
  Twenty-nine consented individuals attending Tel Aviv Sourasky Medical Center for excision of suspected skin lesions, and 21 age- and gender-matched subjects were prospectively recruited. The resected lesions were examined by an expert dermatopathologist. PBLs were isolated from blood samples and protein extracts were subjected to sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The study was double blinded.
  
  
  RESULTS
  CD24 expression in PBLs distinguishes between NMSC and healthy subjects, with high sensitivity (81%) and specificity (67%) for basal cell carcinoma, and 100% and 71%, respectively, for squamous cell carcinoma.
  
  
  CONCLUSIONS
  The CD24 test can successfully distinguish NMSC from healthy subjects. CD24 may serve as a new potential and promising diagnostic biomarker for the detection and surveillance of NMSC.
authors.affiliation: Department of Plastic Surgery, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel.
authors.forename: Ehud
authors.initials: E
authors.lastname: Miller
authors.forename: Shiran
authors.initials: S
authors.lastname: Shapira
authors.forename: Eyal
authors.initials: E
authors.lastname: Gur
authors.forename: Inna
authors.initials: I
authors.lastname: Naumov
authors.forename: Dina
authors.initials: D
authors.lastname: Kazanov
authors.forename: David
authors.initials: D
authors.lastname: Leshem
authors.forename: Yoav
authors.initials: Y
authors.lastname: Barnea
authors.forename: Yaakov
authors.initials: Y
authors.lastname: Meshiach
authors.forename: Andrea
authors.initials: A
authors.lastname: Gat
authors.forename: Daniel
authors.initials: D
authors.lastname: Sion
authors.forename: Nadir
authors.initials: N
authors.lastname: Arber
authors.forename: Sarah
authors.initials: S
authors.lastname: Kraus
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D051927:CD24 Antigen / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D002280:Basal Cell Carcinoma / Q000097:blood* / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D016022:Case-Control Studies; D004311:Double-Blind Method; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011446:Prospective Studies; D012878:Skin Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000235:genetics / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D051927:CD24 Antigen