title: A sensitive and selective quantification of catecholamine neurotransmitters in rat microdialysates by pre-column dansyl chloride derivatization using liquid chromatography-tandem mass spectrometry.
abstract: A rapid and sensitive liquid chromatography tandem mass spectrometry method for simultaneous quantification of catecholamine neurotransmitters in microdialysates was developed. The catecholamine neurotransmitters dopamine (DA) and norepinephrine (NE) were pre-column derivatized with dansyl chloride and analyzed. A gradient elution method was used to separate the analytes from the interferences on an Agilent Poroshell 120 EC-C18 outer porous micro particulate column. The method was robust and sensitive to determine with the lower limit of quantification value of 0.068pmol/mL and 0.059pmol/mL for DA and NE, respectively. It has acceptable precision and accuracy for concentrations over the standard curve range. The method was successfully applied for simultaneous quantitation of DA and NE in the prefrontal cortex (PFC) dialysates of rats obtained from a microdialysis study dosed with vehicle and atomoxetine through intra peritoneal (i.p.) route at a dose of 3mg/kg to monitor the change in extracellular concentrations. Thus, accomplishment of this method would facilitate the neurochemical monitoring for discovery of new chemical entities targeted for the treatment of attention deficit hyperactivity disorder (ADHD).
authors.affiliation: Biopharmaceutical Research, Drug Discovery, Suven Life Sciences Ltd., Serene Chambers, Road - 5, Avenue - 7, Banjara Hills, Hyderabad 500 034, India. ramakrishna_nirogi@yahoo.co.in
authors.forename: Ramakrishna
authors.initials: R
authors.lastname: Nirogi
authors.forename: Prashanth
authors.initials: P
authors.lastname: Komarneni
authors.forename: Vishwottam
authors.initials: V
authors.lastname: Kandikere
authors.forename: Rajeshkumar
authors.initials: R
authors.lastname: Boggavarapu
authors.forename: Gopinadh
authors.initials: G
authors.lastname: Bhyrapuneni
authors.forename: Vijay
authors.initials: V
authors.lastname: Benade
authors.forename: Srinivasarao
authors.initials: S
authors.lastname: Gorentla
mesh_terms: D000818:Animals; D002395:Catecholamines / Q000032:analysis* / Q000134:cerebrospinal fluid / Q000737:chemistry / Q000302:isolation & purification; D002853:Chromatography, Liquid / Q000379:methods*; D003619:Dansyl Compounds / Q000737:chemistry*; D004355:Drug Stability; D008297:Male; D017551:Microdialysis / Q000379:methods*; D018377:Neurotransmitter Agents / Q000032:analysis* / Q000134:cerebrospinal fluid / Q000737:chemistry / Q000302:isolation & purification; D017397:Prefrontal Cortex / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D002395:Catecholamines; D003619:Dansyl Compounds; D018377:Neurotransmitter Agents; C029038:dansyl chloride