title: Construction of an integrated high density simple sequence repeat linkage map in cultivated strawberry (Fragaria × ananassa) and its applicability.
abstract: The cultivated strawberry (Fragaria × ananassa) is an octoploid (2n = 8x = 56) of the Rosaceae family whose genomic architecture is still controversial. Several recent studies support the AAA'A'BBB'B' model, but its complexity has hindered genetic and genomic analysis of this important crop. To overcome this difficulty and to assist genome-wide analysis of F. × ananassa, we constructed an integrated linkage map by organizing a total of 4474 of simple sequence repeat (SSR) markers collected from published Fragaria sequences, including 3746 SSR markers [Fragaria vesca expressed sequence tag (EST)-derived SSR markers] derived from F. vesca ESTs, 603 markers (F. × ananassa EST-derived SSR markers) from F. × ananassa ESTs, and 125 markers (F. × ananassa transcriptome-derived SSR markers) from F. × ananassa transcripts. Along with the previously published SSR markers, these markers were mapped onto five parent-specific linkage maps derived from three mapping populations, which were then assembled into an integrated linkage map. The constructed map consists of 1856 loci in 28 linkage groups (LGs) that total 2364.1 cM in length. Macrosynteny at the chromosome level was observed between the LGs of F. × ananassa and the genome of F. vesca. Variety distinction on 129 F. × ananassa lines was demonstrated using 45 selected SSR markers.
authors.affiliation: Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan. sisobe@kazusa.or.jp
authors.forename: Sachiko N
authors.initials: SN
authors.lastname: Isobe
authors.forename: Hideki
authors.initials: H
authors.lastname: Hirakawa
authors.forename: Shusei
authors.initials: S
authors.lastname: Sato
authors.forename: Fumi
authors.initials: F
authors.lastname: Maeda
authors.forename: Masami
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Toshiki
authors.initials: T
authors.lastname: Mori
authors.forename: Yuko
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Kenta
authors.initials: K
authors.lastname: Shirasawa
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Kimura
authors.forename: Masanobu
authors.initials: M
authors.lastname: Fukami
authors.forename: Fujio
authors.initials: F
authors.lastname: Hashizume
authors.forename: Tomoko
authors.initials: T
authors.lastname: Tsuji
authors.forename: Shigemi
authors.initials: S
authors.lastname: Sasamoto
authors.forename: Midori
authors.initials: M
authors.lastname: Kato
authors.forename: Keiko
authors.initials: K
authors.lastname: Nanri
authors.forename: Hisano
authors.initials: H
authors.lastname: Tsuruoka
authors.forename: Chiharu
authors.initials: C
authors.lastname: Minami
authors.forename: Chika
authors.initials: C
authors.lastname: Takahashi
authors.forename: Tsuyuko
authors.initials: T
authors.lastname: Wada
authors.forename: Akiko
authors.initials: A
authors.lastname: Ono
authors.forename: Kumiko
authors.initials: K
authors.lastname: Kawashima
authors.forename: Naomi
authors.initials: N
authors.lastname: Nakazaki
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Kishida
authors.forename: Mitsuyo
authors.initials: M
authors.lastname: Kohara
authors.forename: Shinobu
authors.initials: S
authors.lastname: Nakayama
authors.forename: Manabu
authors.initials: M
authors.lastname: Yamada
authors.forename: Tsunakazu
authors.initials: T
authors.lastname: Fujishiro
authors.forename: Akiko
authors.initials: A
authors.lastname: Watanabe
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tabata
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D020224:Expressed Sequence Tags; D031985:Fragaria / Q000235:genetics*; D008040:Genetic Linkage; D056426:Genetic Loci; D005819:Genetic Markers; D018745:Genome, Plant; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D059467:Transcriptome
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers