title: Association of abnormal ovarian reserve parameters with a higher incidence of aneuploid blastocysts.
abstract: OBJECTIVE
  To estimate the relationship between hormonal parameters of diminished ovarian reserve and the incidence of aneuploid blastocysts.
  
  
  METHODS
  This prospective cohort trial was performed in a private in vitro fertilization clinic. Three hundred seventy-two patients underwent in vitro fertilization with blastocyst biopsy and aneuploidy screening of all 23 chromosome pairs. Patients were divided into groups based on baseline hormonal ovarian reserve. Group 1 included normal ovarian reserve (n=279) and group 2 included diminished ovarian reserve with day 2 or 3 follicle-stimulating hormone (FSH) more than 10 milli-international units/mL, antimüllerian hormone 1 ng/mL or less (n=93), or both. Patients with diminished ovarian reserves were further subdivided into three groups. Group A included FSH more than 10 milli-international units and antimüllerian hormone 1 ng/mL or less (n=25); group B included FSH more than 10 milli-international units/mL and antimüllerian hormone more than 1 ng/mL (n=34); and group C included antimüllerian hormone 1 ng/mL or less and day 3 FSH less than 10 milli-international units/L (n=34).
  
  
  RESULTS
  Group 2 (diminished ovarian reserve) had a higher percentage of aneuploid blastocysts (66% compared with 51.7%; P<.05) and all aneuploid blastocyst cycles (35.1% compared with 14.3%; P<.001) than group 1 (normal ovarian reserve). However, implantation rates after transfer of euploid blastocysts were similar (69% compared with 61.7%; not significant). The highest percentage of aneuploid blastocysts among diminished ovarian reserve patients was in group A (abnormal FSH and antimüllerian hormone) compared with groups B and C (77.2% compared with 58.5% compared with 58.8%; P<.05). Implantation rates also were no different among the diminished ovarian reserve subgroups (68% compared with 71% compared with 66.7%; not significant).
  
  
  CONCLUSIONS
  Infertility patients with hormonal evidence of diminished ovarian reserve have a significantly higher percentage of aneuploid blastocysts. The combination of abnormal serum FSH and antimüllerian hormone correlated with the greatest rate of embryonic aneuploidy. Regardless of ovarian reserve parameters, transfer of euploid blastocysts resulted in equivalent implantation potential.
  
  
  METHODS
  II.
authors.affiliation: Colorado Center for Reproductive Medicine, Lone Tree, Colorado 80124, USA. mkatz-jaffe@colocrm.com
authors.forename: Mandy G
authors.initials: MG
authors.lastname: Katz-Jaffe
authors.forename: Eric S
authors.initials: ES
authors.lastname: Surrey
authors.forename: Debra A
authors.initials: DA
authors.lastname: Minjarez
authors.forename: Robert L
authors.initials: RL
authors.lastname: Gustofson
authors.forename: John M
authors.initials: JM
authors.lastname: Stevens
authors.forename: William B
authors.initials: WB
authors.lastname: Schoolcraft
mesh_terms: D000328:Adult; D000782:Aneuploidy; D054304:Anti-Mullerian Hormone / Q000097:blood; D001755:Blastocyst; D010064:Embryo Implantation; D004624:Embryo Transfer / Q000379:methods; D004958:Estradiol / Q000097:blood; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D015907:Hysteroscopy; D015994:Incidence; D007247:Infertility, Female / Q000628:therapy; D006080:Ovarian Follicle / Q000000981:diagnostic imaging; D010053:Ovary / Q000503:physiopathology*; D011446:Prospective Studies; D016896:Treatment Outcome; D014463:Ultrasonography
chemical_list: D004958:Estradiol; D054304:Anti-Mullerian Hormone; D005640:Follicle Stimulating Hormone