title: [Collagen fibril formation in vitro at temperatures close to physiological values].
abstract: Fibril formation of collagen from porcine skin was studied at temperatures of 28-39 degrees C. Collagen fibrils obtained in this temperature range have the different degree of order. An optimal temperature for the formation of collagen fibrils is found to be 36.5 degrees C; the structure of fibrils formed at this temperature is more homogeneous than that formed at 34.5 degrees C and 38.5 degrees C. As indicated by electron microscopy data, fibrils with a minimal diameter are formed at physiological values of pH, ionic strength, and temperature. The greater diameter of fibrils formed at 34.5 degrees C is due to a high level of hydration of collagen molecules. Fibril diameter is larger at 38.5 degrees C owing to the cooperative unfolding of the triple helix and a weakening of the binding of collagen molecules.
authors.forename: T I
authors.initials: TI
authors.lastname: Nikolaeva
authors.forename: S M
authors.initials: SM
authors.lastname: Kuznetsova
authors.forename: V V
authors.initials: VV
authors.lastname: Rogachevskiĭ
mesh_terms: D000818:Animals; D024022:Fibrillar Collagens / Q000737:chemistry* / Q000648:ultrastructure*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D008854:Microscopy, Electron; D015394:Molecular Structure; D013552:Swine; D013696:Temperature
chemical_list: D024022:Fibrillar Collagens