title: Cloning of a conserved receptor-like protein kinase gene and its use as a functional marker for homoeologous group-2 chromosomes of the triticeae species.
abstract: Receptor-like kinases (RLKs) play broad biological roles in plants. We report on a conserved receptor-like protein kinase (RPK) gene from wheat and other Triticeae species. The TaRPK1 was isolated from the Triticum aestivum cv. Prins - Triticum timopheevii introgression line IGVI-465 carrying the powdery mildew resistance gene Pm6. The TaRPK1 was mapped to homoeologous chromosomes 2A (TaRPK1-2A), 2D (TaRPK1-2D) and the Pm6-carrier chromosome 2G (TaRPK1-2G) of IGVI-465. Under the tested conditions, only the TaRPK1-2G allele was actively transcribed, producing two distinct transcripts via alternative splicing. The predicted 424-amino acid protein of TaRPK1-2G contained a signal peptide, a transmembrane domain and an intracellular serine/threonine kinase domain, but lacked a typical extracellular domain. The expression of TaRPK1-2G gene was up-regulated upon the infection by Blumeria graminis f.sp. tritici (Bgt) and treatment with methyl jasmonate (MeJA), but down-regulated in response to treatments of SA and ABA. Over-expression of TaRPK1-2G in the powdery mildew susceptible wheat variety Prins by a transient expression assay showed that it slightly reduced the haustorium index of the infected Bgt. These data indicated that TaRPK1-2G participated in the defense response to Bgt infection and in the JA signaling pathway. Phylogenetic analysis indicated that TaRPK1-2G was highly conserved among plant species, and the amino acid sequence similarity of TaRPK1-2G among grass species was more than 86%. Based on its conservation, the RPK gene-based STS primers were designed, and used to amplify the RPK orthologs from the homoeologous group-2 chromosomes of all the tested Triticeae species, such as chromosome 2G of T. timopheevii, 2R of Secale cereale, 2H of Hordeum vulgare, 2S of Aegilops speltoides, 2S(l) of Ae. longissima, 2M(g) of Ae. geniculata, 2S(p) and 2U(p) of Ae. peregrina. The developed STS markers serve as conserved functional markers for the identification of homoeologous group-2 chromosomes of the Triticeae species.
authors.affiliation: The State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Bi
authors.initials: B
authors.lastname: Qin
authors.forename: Tingting
authors.initials: T
authors.lastname: Chen
authors.forename: Aizhong
authors.initials: A
authors.lastname: Cao
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.forename: Liping
authors.initials: L
authors.lastname: Xing
authors.forename: Hongqing
authors.initials: H
authors.lastname: Ling
authors.forename: Daowen
authors.initials: D
authors.lastname: Wang
authors.forename: Chunmei
authors.initials: C
authors.lastname: Yu
authors.forename: Jin
authors.initials: J
authors.lastname: Xiao
authors.forename: Jianhui
authors.initials: J
authors.lastname: Ji
authors.forename: Xueluan
authors.initials: X
authors.lastname: Chen
authors.forename: Peidu
authors.initials: P
authors.lastname: Chen
authors.forename: Dajun
authors.initials: D
authors.lastname: Liu
authors.forename: Xiue
authors.initials: X
authors.lastname: Wang
mesh_terms: D002874:Chromosome Mapping; D002875:Chromosomes / Q000648:ultrastructure; D022202:Chromosomes, Artificial, Bacterial / Q000648:ultrastructure; D032461:Chromosomes, Plant; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D020869:Gene Expression Profiling; D017343:Genes, Plant; D005819:Genetic Markers; D015233:Models, Statistical; D010455:Peptides / Q000737:chemistry; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics; D011110:Polymorphism, Genetic; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D012326:RNA Splicing; D014908:Triticum / Q000235:genetics* / Q000378:metabolism
chemical_list: D005819:Genetic Markers; D010455:Peptides; D010940:Plant Proteins; D011494:Protein Kinases