title: Selective fluorescent detection of RNA in living cells by using imidazolium-based cyclophane.
abstract: A water-soluble imidazolium-based fluorescent chemosensor senses RNA selectively through fluorescence enhancement over other biologically relevant biomolecules in aqueous solution at physiological pH 7.4. Fluorescence image detection of RNA in living cells such as onion cells, HeLa cells, and animal model cells was successfully demonstrated which displays a chelation-enhanced fluorescence effect. These affinities can be attributed to the strong electrostatic (C-H)(+)···A(-) ionic H-bonding and the aromatic moiety driven π-stacking of imidazolium-based cyclophane with the size-complementary major groove of RNA.
authors.affiliation: Center for Superfunctional Materials, Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Korea.
authors.forename: Bahareh
authors.initials: B
authors.lastname: Shirinfar
authors.forename: Nisar
authors.initials: N
authors.lastname: Ahmed
authors.forename: Yu Shin
authors.initials: YS
authors.lastname: Park
authors.forename: Gun-Sik
authors.initials: GS
authors.lastname: Cho
authors.forename: Il Seung
authors.initials: IS
authors.lastname: Youn
authors.forename: Jin-Kwan
authors.initials: JK
authors.lastname: Han
authors.forename: Hong Gil
authors.initials: HG
authors.lastname: Nam
authors.forename: Kwang S
authors.initials: KS
authors.lastname: Kim
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000737:chemistry* / Q000166:cytology; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007093:Imidazoles / Q000737:chemistry*; D047028:Macrocyclic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D019697:Onions / Q000737:chemistry* / Q000166:cytology; D012313:RNA / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D007093:Imidazoles; D047028:Macrocyclic Compounds; D012313:RNA