title: Adsorption of milk proteins on to calcium phosphate particles.
abstract: The adsorption of caseins from sodium caseinate (SC) and whey proteins from whey protein isolate (WPI) on to particles of hydroxyapatite (HA) was studied. Confocal microscopy and zeta-potential measurements showed that both caseins and whey proteins bound to HA, resulting in an increase in the absolute value of the zeta-potential of the particles. This adsorption improved the suspension stability of the HA particles in water. For both protein sources, there was a preference in the protein adsorption: in the order β-casein > α(s)-casein > κ-casein for sodium-caseinate-coated particles; in the order β-lactoglobulin > α-lactalbumin for WPI-coated particles. The adsorption of caseins and whey proteins on to HA could be fitted using a simple Langmuir model, suggesting a single layer adsorption of caseins and whey proteins on to the HA surface. Possible mechanisms involved in the interaction between milk proteins and HA are discussed, in relation to the structure and the surface properties of both milk proteins and HA particles.
authors.affiliation: Riddet Institute, Massey University, Private Bag 11 222, Palmerston North 4442, New Zealand.
authors.forename: Lucile
authors.initials: L
authors.lastname: Tercinier
authors.forename: Aiqian
authors.initials: A
authors.lastname: Ye
authors.forename: Skelte
authors.initials: S
authors.lastname: Anema
authors.forename: Anne
authors.initials: A
authors.lastname: Singh
authors.forename: Harjinder
authors.initials: H
authors.lastname: Singh
mesh_terms: D000327:Adsorption; D000818:Animals; D002364:Caseins / Q000302:isolation & purification; D017886:Durapatite / Q000737:chemistry*; D008894:Milk Proteins / Q000302:isolation & purification*; D008956:Models, Chemical; D000067816:Whey Proteins
chemical_list: D002364:Caseins; D008894:Milk Proteins; D000067816:Whey Proteins; D017886:Durapatite