title: PCR-RFLP based method for molecular differentiation of sand fly species Phlebotomus argentipes, Phlebotomus papatasi, and Sergentomyia babu found in India.
abstract: PCR-Restriction fragment length polymorphism is a time saving and accurate technique to differentiate closely related organisms. In the regions endemic for visceral leishmaniasis in India, various species of morphological similar sand fly exist but only female Phlebotomus argentipes is the vector for visceral leishmaniasis. In the current study primers were designed targeting the 18S rRNA encoding gene that showed amplification in all the major sand fly species found in India. The amplified fragments were further digested using the HinfI or HpaII restriction enzymes. Each of the restriction enzyme produced a species specific restriction patterns, which can easily be used to identify specific sand fly species. This technique can be used in the identification of sand fly species.
authors.affiliation: Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, U.P., India.
authors.forename: Puja
authors.initials: P
authors.lastname: Tiwary
authors.forename: Dinesh
authors.initials: D
authors.lastname: Kumar
authors.forename: Madhukar
authors.initials: M
authors.lastname: Rai
authors.forename: Shyam
authors.initials: S
authors.lastname: Sundar
mesh_terms: D000818:Animals; D001483:Base Sequence; D005260:Female; D007194:India; D008969:Molecular Sequence Data; D010691:Phlebotomus / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D013045:Species Specificity
chemical_list: D012337:RNA, Ribosomal, 18S