title: Induction of vitellogenin synthesis in immature male yellowfin seabream (Acanthopagrus latus) exposed to 4-nonylphenol and 17β-estradiol.
abstract: In the present study, the effects of xenoestrogen 4-nonylphenol (4-NP) and 17β-estradiol (E2) on the synthesis of vitellogenin (VTG) in immature male yellowfin seabream (Acanthoparus latus) were assessed to determine the potential use of this species as a biological model for studying endocrine disrupters in the Musa estuary in southern seawaters of Iran. A total number of 104 fish were injected by 10, 50, 100, 150 and 200 µg g(-) (1) week(-) (1) of 4-NP and 2 µg g(-) (1) week(-) (1) of E2, and their blood plasma was obtained for biochemical analysis on days 0, 7 and 14. The hepatosomatic index (HSI) of each fish was also recorded at each sampling time. The results showed that 4-NP significantly induced VTG synthesis in A. latus, which was detected by gel electrophoresis as two major protein bands (∼210 and 191 KDa). The induction of VTG was also determined indirectly by measuring the alkali-labile phosphate, total plasma calcium and protein. All of these VTG indicators significantly increased in the plasma of 4-NP- and E2-treated fish. Meanwhile, a significant dose-dependent increase was observed in HSI, indicating the activation of hepatic VTG production in 4-NP- and E2-injected fish. Furthermore, the plasma alanine aminotransferase and aspartate aminotransferase concentrations significantly increased in 4-NP-treated fish, presumably reflecting a situation of hepatic tissue damage due to 4-NP toxicity. Based on the results, it is suggested that A. latus could be used successfully as a model species for future studies of endocrine disrupting contaminants in the Musa estuary.
authors.affiliation: Department of Marine Biology, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, Khorramshahr, Islamic Republic of Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Naderi
authors.affiliation: Department of Marine Biology, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, Khorramshahr, Islamic Republic of Iran a.safahieh@kmsu.ac.ir.
authors.forename: Alireza
authors.initials: A
authors.lastname: Safahieh
authors.affiliation: Department of Marine Ecology, South of Iran Aquaculture Research Center, Ahvaz, Islamic Republic of Iran.
authors.forename: Simin Dehghan
authors.initials: SD
authors.lastname: Madiseh
authors.affiliation: Department of Marine Biology, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, Khorramshahr, Islamic Republic of Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Zolgharnein
authors.affiliation: Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Islamic Republic of Iran.
authors.forename: Ebrahim Rajabzade
authors.initials: ER
authors.lastname: Ghatrami
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000633:toxicity*; D004958:Estradiol / Q000097:blood / Q000633:toxicity*; D007492:Iran; D008099:Liver / Q000378:metabolism*; D008297:Male; D023421:Models, Animal; D021541:Sea Bream / Q000378:metabolism*; D014819:Vitellogenins / Q000096:biosynthesis*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D052244:Endocrine Disruptors; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D004958:Estradiol