title: [High-mobility group box protein 1 in synergy with interleukin-1β promotes interleukin-8 expression in human airway epithelial cells in vitro].
abstract: OBJECTIVE
  To test the effect of high-mobility group box protein 1 (HMGB1) alone or in synergy with interleukin-1β (IL-1β) on the expression of IL-8 in human airway epithelial cells in vitro.
  
  
  METHODS
  Human airway epithelial 16HBE and A549 cell lines were incubated with HMGB1 (100 ng/ml) in the absence or presence of IL-1β (10 ng/ml) for 24 h, and the changes of IL-8 mRNA and protein expressions were assessed using quantitative PCR and enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  In the two human airway epithelial cell lines, HMGB1 alone did not produce obvious effect on the expression of IL-8, but in the presence of IL-1β, HMGB1 caused a significant increase of IL-8 expressions at both the mRNA and protein levels.
  
  
  CONCLUSIONS
  HMGB1 in synergy with IL-1β increases the expression of IL-8 in human airway epithelial cells, which provides new evidence that HMGB1 contributes to neutrophilic airway inflammation by regulating IL-8 expression.
authors.affiliation: Department of Respiratory and Critical Care Medicine, Chronic Airway Disease Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. medidan@163.com
authors.forename: Dandan
authors.initials: D
authors.lastname: Zhang
authors.forename: Haijin
authors.initials: H
authors.lastname: Zhao
authors.forename: Liqin
authors.initials: L
authors.lastname: Zhou
authors.forename: Jiafu
authors.initials: J
authors.lastname: Song
authors.forename: Hangming
authors.initials: H
authors.lastname: Dong
authors.forename: Fei
authors.initials: F
authors.lastname: Zou
authors.forename: Shaoxi
authors.initials: S
authors.lastname: Cai
mesh_terms: D001980:Bronchi / Q000166:cytology / Q000187:drug effects*; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D024243:HMGB1 Protein / Q000494:pharmacology*; D006801:Humans; D007249:Inflammation; D053583:Interleukin-1beta / Q000494:pharmacology*; D016209:Interleukin-8 / Q000378:metabolism*; D012333:RNA, Messenger
chemical_list: C508606:CXCL8 protein, human; D024243:HMGB1 Protein; D053583:Interleukin-1beta; D016209:Interleukin-8; D012333:RNA, Messenger