title: Engineered rRNA enhances the efficiency of selenocysteine incorporation during translation.
abstract: We developed a new genetic selection approach to screen for mutations that can alter the efficiency of selenocysteine incorporation. We identified mutations in 16S rRNA that increase or decrease the efficiency of selenocysteine incorporation in  Escherichia coli  without influencing the efficiency or fidelity of canonical translation. Engineered ribosomes with improved selenocysteine incorporation provide valuable tools for synthetic biology and biotechnology.
authors.affiliation: Western Australian Institute for Medical Research and Centre for Medical Research, The University of Western Australia, Australia.
authors.forename: Ross
authors.initials: R
authors.lastname: Thyer
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Filipovska
authors.forename: Oliver
authors.initials: O
authors.lastname: Rackham
mesh_terms: D004926:Escherichia coli / Q000235:genetics; D005818:Genetic Engineering; D009154:Mutation; D014176:Protein Biosynthesis / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics*; D017279:Selenocysteine / Q000737:chemistry / Q000235:genetics*
chemical_list: D012336:RNA, Ribosomal, 16S; D017279:Selenocysteine