title: Usutu virus growth in human cell lines: induction of and sensitivity to type I and III interferons.
abstract: The mechanisms of Usutu virus (USUV) pathogenesis are largely unknown. The aim of this study was to evaluate the sensitivity of USUV to interferon (IFN) and the capacity of USUV to stimulate IFN production. Initial experiments were conducted to characterize the susceptibility of human cell lines to USUV infection and to evaluate the single-growth cycle replication curve of USUV. Results indicate that USUV is able to infect a variety of human cell lines, completing the replication cycle in Hep-2 and Vero cells within 48 h. Pre-treatment of cells with types I and III IFNs significantly inhibited the replication of USUV. However, the inhibitory effects of IFNs were considerably less if IFN was added after viral infection had been initiated. Also, USUV weakly induced types I and III IFNs.
authors.affiliation: Department of Molecular Medicine, Laboratory of Virology, 'Sapienza' University of Rome, Viale di Porta Tiburtina 28, 00185, Rome, Italy.
authors.forename: Carolina
authors.initials: C
authors.lastname: Scagnolari
authors.affiliation: Parasitology Unit, Department of Public Health and Infectious Diseases, 'Sapienza' University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Beniamino
authors.initials: B
authors.lastname: Caputo
authors.affiliation: Department of Molecular Medicine, Laboratory of Virology, 'Sapienza' University of Rome, Viale di Porta Tiburtina 28, 00185, Rome, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Trombetti
authors.affiliation: Department of Molecular Medicine, Laboratory of Virology, 'Sapienza' University of Rome, Viale di Porta Tiburtina 28, 00185, Rome, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Cacciotti
authors.affiliation: Department of Molecular Medicine, Laboratory of Virology, 'Sapienza' University of Rome, Viale di Porta Tiburtina 28, 00185, Rome, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Soldà
authors.affiliation: Department of Molecular Medicine, Laboratory of Virology, 'Sapienza' University of Rome, Viale di Porta Tiburtina 28, 00185, Rome, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Spano
authors.affiliation: Department of Public Health and Infectious Diseases, 'Sapienza' University of Rome, Viale Regina Elena 324, 00185 Rome, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Villari
authors.affiliation: Parasitology Unit, Department of Public Health and Infectious Diseases, 'Sapienza' University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Della Torre
authors.affiliation: Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University, PO Box 35, Al-Khod, Muscat 123, Oman.|Viral Zoonoses, Emerging and Vector-Borne Infections Group, Institute of Virology, University of Veterinary Medicine Vienna, Veterinärplatz 1, A-1210 Vienna, Austria.
authors.forename: Norbert
authors.initials: N
authors.lastname: Nowotny
authors.affiliation: Department of Molecular Medicine, Laboratory of Virology, 'Sapienza' University of Rome, Viale di Porta Tiburtina 28, 00185, Rome, Italy.
authors.forename: Guido
authors.initials: G
authors.lastname: Antonelli
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D018349:Encephalitis Viruses, Japanese / Q000254:growth & development* / Q000276:immunology*; D006801:Humans; D007372:Interferons / Q000276:immunology* / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D007372:Interferons