title: [Application of melting curve to analyze genotype of Duffy blood group antigen Fy-a/b].
abstract: This study was aimed to establish the real-time multiple-PCR with melting curve analysis for Duffy blood group Fy-a/b genotyping. According to the sequence of mRNA coding for β-actin and Fy-a/b, the primers of β-actin and Fy-a/b were synthesized. The real-time multiple-PCR with melting curve analysis for Fy-a/b genotyping was established. The Fy-a/b genotyping of 198 blood donors in Chinese Chengdu area has been investigated by melting curve analysis and PCR-SSP. The results showed that the results of Fy-a/b genotype by melting curve analysis were consistent with PCR-SSP. In all of 198 donors in Chinese Chengdu, 178 were Fy(a) (+) (89.9%), 19 were Fy(a) (+) Fy(b) (+) (9.6%), and 1 was Fy(b) (+) (0.5%). The gene frequency of Fy(a) was 0.947, while that of Fy(b) was 0.053. It is concluded that the genotyping method of Duffy blood group with melting curve analysis is established, which can be used as a high-throughput screening tool for Duffy blood group genotyping; and the Fy(a) genotype is the major of Duffy blood group of donors in Chinese Chengdu area.
authors.affiliation: Blood Research Laboratory, Chengdu Blood Center, Chengdu 610041, Sichuan Province, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Chen
authors.forename: Chang-Hua
authors.initials: CH
authors.lastname: Zhou
authors.forename: Ying
authors.initials: Y
authors.lastname: Hong
authors.forename: Tian-Xiang
authors.initials: TX
authors.lastname: Gong
mesh_terms: D000483:Alleles; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D004375:Duffy Blood-Group System / Q000235:genetics*; D005615:Freezing; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D004375:Duffy Blood-Group System