title: Inhibitory activity of surfactin, produced by different Bacillus subtilis subsp. subtilis strains, against Listeria monocytogenes sensitive and bacteriocin-resistant strains.
abstract: Three surfactin-producing Bacillus subtilis strains, C4, M1 and G2III, previously isolated from honey and intestines from the Apis mellifera L. bee, were phylogenetically characterized at sub-species level as B. subtilis subsp. subtilis using gyrA gene sequencing. The antagonistic effect of surfactin was studied against seven different Listeria monocytogenes strains, 6 of which were resistant to bacteriocins. Surfactin showed anti-Listeria activity against all 7 strains and a dose of 0.125 mg/mL of surfactin was enough to inhibit this pathogen. Surfactin sintetized by B. subtilis subsp. subtilis C4 inhibited the pathogen in lower concentrations, 0.125 mg/mL, followed by G2III and M1 with 0.25 and 1mg/mL, respectively. In particular, a dose of 0.125 mg/mL reduced the viability of L. monocytogenes 99/287 RB6, a bacteriocin-resistant strain, to 5 log orders. Surfactin assayed maintained anti-Listeria activity within a pH range of between 2 and 10, after heat treatment (boiling for 10 min and autoclaving at 121 °C for 15 min) and after treatment with proteolytic enzymes. These results suggest that surfactin can be used as a new tool for prevention and the control of L. monocytogenes in different environments, for example, in the food industry.
authors.affiliation: Instituto de Investigaciones para la Industria Química (INIQUI-CONICET), Universidad Nacional de Salta, Argentina.
authors.forename: Daniela C
authors.initials: DC
authors.lastname: Sabaté
authors.forename: M Carina
authors.initials: MC
authors.lastname: Audisio
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D001412:Bacillus subtilis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001516:Bees / Q000382:microbiology; D016000:Cluster Analysis; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D041981:Gastrointestinal Tract / Q000382:microbiology; D006722:Honey / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D055666:Lipopeptides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D008089:Listeria monocytogenes / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000378:metabolism; D010456:Peptides, Cyclic / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D010802:Phylogeny; D055550:Protein Stability; D017422:Sequence Analysis, DNA; D013501:Surface-Active Agents / Q000378:metabolism* / Q000494:pharmacology; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D055666:Lipopeptides; D010456:Peptides, Cyclic; D013501:Surface-Active Agents; C009365:surfactin peptide; D010447:Peptide Hydrolases; D027081:DNA Gyrase