title: [Determination of ten basic dyes in meat products by ultra fast liquid chromatography-ion trap time of flight mass spectrometry].
abstract: A method was developed for the simultaneous determination of 10 basic dyes in meat products using ultra fast liquid chromatography-ion trap time of flight mass spectrometry (LC-IT-TOF-MS). The target analytes were separated at a flow rate of 0.2 mL/min on a Waters Acquity UPLC BEH C18 (100 mm x 2.1 mm, 1.7 microm) column with a gradient elution. The mobile phase was 5 mmol/L ammonium acetate-acetonitrile (containing 0.1% (v/v) formic acid). The identification and quantification were achieved in positive ion mode with electro spray ionization source. The samples were extracted with a simple procedure using acetonitrile and cleaned up by weak cation exchange (Oasis WCX) solid phase extraction column. Ten basic dyes were determined by LC-IT-TOF-MS, and quantified by external standard method. The developed method showed a good linearity over the wide range of 1.0 - 100.0 microg/L, and the relative standard deviations (n = 7) were less than 8.54%. The average recoveries of the ten basic dyes at three levels (2, 10 and 25 microg/kg) were ranged from 65.39% to 119.18%. Therefore, this method, owing to its simplicity, rapidity and high sensitivity, has a good applicability to the simultaneous determination of dye residues in meat products.
authors.affiliation: Zhejiang Institute of Quality Inspection Science, Hangzhou 310013, China. fyzdl@163.com
authors.forename: Donglei
authors.initials: D
authors.lastname: Zhang
authors.forename: Lina
authors.initials: L
authors.lastname: Wang
authors.forename: Xiaozhen
authors.initials: X
authors.lastname: Chen
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Cao
authors.forename: Liying
authors.initials: L
authors.lastname: Huang
mesh_terms: D000818:Animals; D001576:Benzophenoneidum / Q000032:analysis; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D004396:Coloring Agents / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D012235:Rhodamines / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013552:Swine
chemical_list: D004396:Coloring Agents; D012235:Rhodamines; D001576:Benzophenoneidum; C029773:rhodamine B