title: Specific DNA detection using antibody mediated fluorescence quenching.
abstract: First homogenous immunoassay for sequence-specific nucleic acid detection is developed. The assay bases on our finding that a fluorophore inserted into a DNA probe instead of one of the internal nucleotides may get protected from fluorescence quenching caused by an anti-fluorophore antibody, if the probe is hybridized with the target sequence. This ensures a positive signal in the antibody presence. The assay enables quantitative detection and may have potential for development of homogenous high-throughput platforms.
authors.affiliation: UP Transfer GmbH, Am Neuen Palais 10, 14469 Potsdam, Germany. frank.sellrie@up-transfer.de
authors.forename: Frank
authors.initials: F
authors.lastname: Sellrie
authors.forename: Elmara
authors.initials: E
authors.lastname: Graser
authors.forename: Christine
authors.initials: C
authors.lastname: Lenz
authors.forename: Timo
authors.initials: T
authors.lastname: Hillebrand
authors.forename: Jörg A
authors.initials: JA
authors.lastname: Schenk
mesh_terms: D000974:Antibodies, Antinuclear / Q000235:genetics; D000911:Antibodies, Monoclonal; D015374:Biosensing Techniques / Q000379:methods; D004247:DNA / Q000276:immunology / Q000302:isolation & purification*; D005456:Fluorescent Dyes / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D009693:Nucleic Acid Hybridization; D019779:Salmonella enterica / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D004247:DNA