title: Apoferritin protein nanoparticles dually labeled with aptamer and horseradish peroxidase as a sensing probe for thrombin detection.
abstract: A novel and ultrasensitive sandwich-type electrochemical aptasensor has been developed for the detection of thrombin, based on dual signal-amplification using HRP and apoferritin. Core/shell Fe(3)O(4)/Au magnetic nanoparticles (AuMNPs) loading aptamer1 (Apt1) was used as recognition elements, and apoferritin dually labeled with Aptamer2 (Apt2) and HRP was used as a detection probe. Sandwich-type complex, Apt1/thrombin/Apt2-apoferritin NPs-HRP was formed by the affinity reactions between AuMNPs-Apt1, thrombin, and Apt2-apoferritin-HRP. The complex was anchored on a screen-printed carbon electrode (SPCE). Differential pulse voltammetry (DPV) was used to monitor the electrode response. The proposed aptasensor yielded a linear current response to thrombin concentrations over a broad range of 0.5-100 pM with a detection limit of 0.07 pM (S/N=3). The detection signal was amplified by using apoferritin and HRP. This nanoparticle-based aptasensor offers a new method for rapid, sensitive, selective, and inexpensive quantification of thrombin, and offers a promising potential in protein detection and disease diagnosis.
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.forename: Meiling
authors.initials: M
authors.lastname: Liu
authors.forename: Youyu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Haitao
authors.initials: H
authors.lastname: Li
authors.forename: Yuehe
authors.initials: Y
authors.lastname: Lin
authors.forename: Shouzhuo
authors.initials: S
authors.lastname: Yao
mesh_terms: D000818:Animals; D001052:Apoferritins / Q000737:chemistry / Q000378:metabolism*; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000378:metabolism*; D015374:Biosensing Techniques / Q000295:instrumentation*; D055664:Electrochemical Techniques / Q000295:instrumentation; D004867:Equipment Design; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D013917:Thrombin / Q000032:analysis* / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; D055767:Immobilized Proteins; D058185:Magnetite Nanoparticles; D001052:Apoferritins; D006735:Horseradish Peroxidase; D013917:Thrombin