title: Diagnostic usefulness of p16/CDKN2A FISH in distinguishing between sarcomatoid mesothelioma and fibrous pleuritis.
abstract: The distinction between sarcomatoid mesothelioma and fibrous pleuritis is difficult based on histology, especially when the amount of tumor tissue examined via biopsy is small and immunohistochemical examination is inconclusive. We studied the usefulness of deletion of p16 with fluorescence in situ hybridization (FISH) and p16 hypermethylation with polymerase chain reaction for the diagnosis and prognosis of malignant pleural mesothelioma (MPM). We analyzed 50 MPMs, including 22 sarcomatoid mesothelioma cases and 10 fibrous pleuritis cases. We set the cutoff value of homozygous deletion pattern as 14.4% based on FISH signaling patterns using samples of fibrous pleuritis. The percentage of homozygous deletion pattern was higher than 14.4% in 55.6% of the epithelioid mesotheliomas (10/18) and in all of the sarcomatoid mesotheliomas (22/22). Methylation of p16 was observed in 7 (20.6%) of 34 informative cases. p16 FISH analysis can be a reliable test for distinguishing between sarcomatoid mesothelioma and fibrous pleuritis and a prognostic factor for MPM.
authors.affiliation: Dept of Pathology, Tokyo Women's Medical University Yachiyo Medical Center, Owada-Shinden, Yachiyo, Chiba, Japan.
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.forename: Kenzo
authors.initials: K
authors.lastname: Hiroshima
authors.forename: Shinji
authors.initials: S
authors.lastname: Matsumoto
authors.forename: Kazuki
authors.initials: K
authors.lastname: Nabeshima
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Yusa
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ozaki
authors.forename: Michio
authors.initials: M
authors.lastname: Fujino
authors.forename: Hisami
authors.initials: H
authors.lastname: Yamakawa
authors.forename: Yukio
authors.initials: Y
authors.lastname: Nakatani
authors.forename: Yuji
authors.initials: Y
authors.lastname: Tada
authors.forename: Hideaki
authors.initials: H
authors.lastname: Shimada
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Tagawa
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics* / Q000378:metabolism; D019175:DNA Methylation; D003937:Diagnosis, Differential; D005260:Female; D005355:Fibrosis / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D008297:Male; D008654:Mesothelioma / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D000086002:Mesothelioma, Malignant; D008875:Middle Aged; D010997:Pleural Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D010998:Pleurisy / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D011379:Prognosis; D012509:Sarcoma / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D019941:Cyclin-Dependent Kinase Inhibitor p16