title: Oxidative metabolites of curcumin poison human type II topoisomerases.
abstract: The polyphenol curcumin is the principal flavor and color component of the spice turmeric. Beyond its culinary uses, curcumin is believed to positively impact human health and displays antioxidant, anti-inflammatory, antibacterial, and chemopreventive properties. It also is in clinical trials as an anticancer agent. In aqueous solution at physiological pH, curcumin undergoes spontaneous autoxidation that is enhanced by oxidizing agents. The reaction proceeds through a series of quinone methide and other reactive intermediates to form a final dioxygenated bicyclopentadione product. Several naturally occurring polyphenols that can form quinones have been shown to act as topoisomerase II poisons (i.e., they increase levels of topoisomerase II-mediated DNA cleavage). Because several of these compounds have chemopreventive properties, we determined the effects of curcumin, its oxidative metabolites, and structurally related degradation products (vanillin, ferulic acid, and feruloylmethane) on the DNA cleavage activities of human topoisomerase IIα and IIβ. Intermediates in the curcumin oxidation pathway increased the level of DNA scission mediated by both enzymes ~4-5-fold. In contrast, curcumin and the bicyclopentadione, as well as vanillin, ferulic acid, and feruloylmethane, had no effect on DNA cleavage. As found for other quinone-based compounds, curcumin oxidation intermediates acted as redox-dependent (as opposed to interfacial) topoisomerase II poisons. Finally, under conditions that promote oxidation, the dietary spice turmeric enhanced topoisomerase II-mediated DNA cleavage. Thus, even within the more complex spice formulation, oxidized curcumin intermediates appear to function as topoisomerase II poisons.
authors.affiliation: Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
authors.forename: Adam C
authors.initials: AC
authors.lastname: Ketron
authors.forename: Odaine N
authors.initials: ON
authors.lastname: Gordon
authors.forename: Claus
authors.initials: C
authors.lastname: Schneider
authors.forename: Neil
authors.initials: N
authors.lastname: Osheroff
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D030024:Curcuma / Q000737:chemistry / Q000378:metabolism; D003474:Curcumin / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D053837:DNA Cleavage / Q000187:drug effects*; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D006801:Humans; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D004396:Coloring Agents; D010936:Plant Extracts; C012596:turmeric extract; D004250:DNA Topoisomerases, Type II; D003474:Curcumin