title: Ephrin-A1 mRNA is associated with poor prognosis of colorectal cancer.
abstract: We previously studied hypoxic tumor cells from hepatic metastases of colorectal             cancer (CRC) and determined several potential prognostic factors, including expression             of ephrin-A1 (EFNA1), which was highly induced by hypoxia. Here, we further evaluated             the prognostic impact of EFNA1 expression. Samples from a total of 366 CRC patients             from 11 institutes were analyzed by either microarray (n=220) or quantitative             reverse-transcriptase polymerase chain reaction (n=146). EFNA1 was an independent             prognostic factor for CRC (p<0.05). In vitro assays revealed that loss of EFNA1             following siRNA treatment was associated with reduced proliferative activity and             decreased invasion and migration of CRC cell lines. EFNA1 expression is a useful             marker for predicting high risk of relapse and cancer-related death in patients             who have undergone curative resection for CRC.
authors.affiliation: Department of Surgery, Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Osaka, Japan. hyamamoto@gesurg.med.osaka-u.ac.jp
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Tei
authors.forename: Mamoru
authors.initials: M
authors.lastname: Uemura
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takemasa
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Uemura
authors.forename: Kohei
authors.initials: K
authors.lastname: Murata
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Fukunaga
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ohue
authors.forename: Tadashi
authors.initials: T
authors.lastname: Ohnishi
authors.forename: Kimimasa
authors.initials: K
authors.lastname: Ikeda
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kato
authors.forename: Shu
authors.initials: S
authors.lastname: Okamura
authors.forename: Masakazu
authors.initials: M
authors.lastname: Ikenaga
authors.forename: Naotsugu
authors.initials: N
authors.lastname: Haraguchi
authors.forename: Junichi
authors.initials: J
authors.lastname: Nishimura
authors.forename: Tsunekazu
authors.initials: T
authors.lastname: Mizushima
authors.forename: Koshi
authors.initials: K
authors.lastname: Mimori
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Doki
authors.forename: Masaki
authors.initials: M
authors.lastname: Mori
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D036382:Ephrin-A1 / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D045325:HCT116 Cells; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D012008:Recurrence
chemical_list: D014408:Biomarkers, Tumor; D036382:Ephrin-A1; D012333:RNA, Messenger