title: Formation of ferric oxyhydroxide nanoparticles mediated by peptides in anchovy (Engraulis japonicus) muscle protein hydrolysate.
abstract: Nanosized iron fortificants appear to be promising and can be synthesized in a greener way using peptides as biotemplates. Anchovy is a huge underdeveloped source of muscle protein that enhances human nonheme iron absorption. This paper shows that peptides in anchovy ( Engraulis japonicus ) muscle protein hydrolysate (AMPH) mediate the formation of monodispersed ferric oxyhydroxide nanoparticles (FeONPs) with diameters of 20-40 nm above pH 3.0. Peptides in AMPH nucleate iron through carboxyl groups and crystal growth then occur as a result of condensation of carboxylate-ligated hydroxide iron centers, yielding Fe-O-Fe cross-link bonds. Monomers of FeONPs are formed after steric obstruction of further crystal growth by peptide backbones with certain lengths and further stabilized by surface-adsorbed peptides. The iron-loading capacity of peptides in AMPH is up to 27.5 mg iron/g peptide. Overall, the present study provides a greener alternative route to the synthesis of FeONPs.
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province 266003, China.
authors.forename: Haohao
authors.initials: H
authors.lastname: Wu
authors.forename: Zunying
authors.initials: Z
authors.lastname: Liu
authors.forename: Shiyuan
authors.initials: S
authors.lastname: Dong
authors.forename: Yuanhui
authors.initials: Y
authors.lastname: Zhao
authors.forename: Hai
authors.initials: H
authors.lastname: Huang
authors.forename: Mingyong
authors.initials: M
authors.lastname: Zeng
mesh_terms: D000818:Animals; D005290:Ferric Compounds / Q000737:chemistry*; D005399:Fishes / Q000378:metabolism*; D006801:Humans; D053768:Metal Nanoparticles; D046529:Microscopy, Electron, Transmission; D009124:Muscle Proteins / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry*; D059748:Proteolysis
chemical_list: D005290:Ferric Compounds; D009124:Muscle Proteins; D010455:Peptides; C092844:ferric oxyhydroxide