title: Apoptotic regulators promote cytokinetic midbody degradation in C. elegans.
abstract: Cell death genes are essential for apoptosis and other cellular events, but their nonapoptotic functions are not well understood. The midbody is an important cytokinetic structure required for daughter cell abscission, but its fate after cell division remains elusive in metazoans. In this paper, we show through live-imaging analysis that midbodies generated by Q cell divisions in Caenorhabditis elegans were released to the extracellular space after abscission and subsequently internalized and degraded by the phagocyte that digests apoptotic Q cell corpses. We further show that midbody degradation is defective in apoptotic cell engulfment mutants. Externalized phosphatidylserine (PS), an engulfment signal for corpse phagocytosis, exists on the outer surface of the midbody, and inhibiting PS signaling delayed midbody clearance. Thus, our findings uncover a novel function of cell death genes in midbody internalization and degradation after cell division.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Chai
authors.forename: Dong
authors.initials: D
authors.lastname: Tian
authors.forename: Yihong
authors.initials: Y
authors.lastname: Yang
authors.forename: Guoxin
authors.initials: G
authors.lastname: Feng
authors.forename: Ze
authors.initials: Z
authors.lastname: Cheng
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Guangshuo
authors.initials: G
authors.lastname: Ou
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D019943:Amino Acid Substitution; D026905:Amino Acid Transport Systems / Q000378:metabolism; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D017173:Caenorhabditis elegans / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D048749:Cytokinesis; D004847:Epithelial Cells / Q000378:metabolism / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000096:biosynthesis; D007700:Kinetics; D007814:Larva / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D016297:Mutagenesis, Site-Directed; D010587:Phagocytosis; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015398:Signal Transduction; D059008:Time-Lapse Imaging; D020691:rab GTP-Binding Proteins / Q000378:metabolism
chemical_list: D018528:ATP-Binding Cassette Transporters; D026905:Amino Acid Transport Systems; D051017:Apoptosis Regulatory Proteins; C112888:CED-6 protein, C elegans; C112887:CED-7 protein, C elegans; D029742:Caenorhabditis elegans Proteins; D008565:Membrane Proteins; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; C422043:ced-1 protein, C elegans; C573923:ctns-1 protein, C elegans; D049452:Green Fluorescent Proteins; D020691:rab GTP-Binding Proteins