title: Microbacterium lemovicicum sp. nov., a bacterium isolated from a natural uranium-rich soil.
abstract: An actinobacterial strain, designated ViU22(T), was isolated from a natural uranium-rich soil and was studied using a polyphasic approach. Cells formed orange-pigmented colonies, were rod-shaped, Gram-positive (non-staining method), non-motile and non-spore-forming. This organism grew in 0-4.5 % (w/v) NaCl and at 15-37 °C, with optimal growth occurring in 0.5 % (w/v) NaCl and at 30 °C. Comparative 16S rRNA gene sequence analysis revealed that the strain ViU22(T) belonged to the genus Microbacterium. It exhibited highest 16S rRNA gene sequence similarity with the type strains of Microbacterium testaceum (98.14 %) and Microbacterium binotii (98.02 %). The DNA-DNA relatedness of strains ViU22(T) with the most closely related type strains Microbacterium testaceum and Microbacterium binotii DSM 19164(T) was 20.10 % (± 0.70) and 28.05 % (± 0.35), respectively. Strain ViU22(T) possessed a type B2β peptidoglycan with partial substitution of glutamic acid by 3-hydroxy glutamic acid. The major menaquinones were MK-11 and MK-12. Major polar lipids detected in the strain ViU22(T) were diphosphatidylglycerol, phosphatidylglycerol, an unknown phospholipid and unknown glycolipids. The predominant fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0, a pattern reported for other Microbacterium species. The major cell-wall sugars were galactose, xylose and mannose and the DNA G+C content was 71 mol%. Together, the DNA-DNA hybridization results and the differentiating phenotypic characteristics, showed that strain ViU22(T) should be classified as the type strain of a novel species within the genus Microbacterium, for which the name Microbacterium lemovicicum sp. nov. is proposed. The type strain is ViU22(T) ( = ATCC BAA-2396(T) = CCUG 62198(T) = DSM 25044(T)).
authors.affiliation: Université d'Aix-Marseille, 13108 Saint-Paul-lez-Durance, France.|CNRS, UMR 7265, 13108 Saint-Paul-lez-Durance, France.|CEA, DSV, IBEB, SBVME, LIPM, 13108 Saint-Paul-lez-Durance, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Mondani
authors.affiliation: Université d'Aix-Marseille, 13108 Saint-Paul-lez-Durance, France.|CNRS, UMR 7265, 13108 Saint-Paul-lez-Durance, France.|CEA, DSV, IBEB, SBVME, LIPM, 13108 Saint-Paul-lez-Durance, France.
authors.forename: Laurie
authors.initials: L
authors.lastname: Piette
authors.affiliation: CNRS, UMR 7138, Systématique, Adaptation, Evolution, Parc Valrose, BP71, 06108 Nice cedex 02, France.|Université de Nice-Sophia Antipolis, UMR 7138 Systématique, Adaptation, Evolution, Parc Valrose, BP71, 06108 Nice cedex 02, France.
authors.forename: Richard
authors.initials: R
authors.lastname: Christen
authors.affiliation: CNRS, UMR 7138, Systématique, Adaptation, Evolution, Parc Valrose, BP71, 06108 Nice cedex 02, France.|Université de Nice-Sophia Antipolis, UMR 7138 Systématique, Adaptation, Evolution, Parc Valrose, BP71, 06108 Nice cedex 02, France.
authors.forename: Dipankar
authors.initials: D
authors.lastname: Bachar
authors.affiliation: Université d'Aix-Marseille, 13108 Saint-Paul-lez-Durance, France.|CNRS, UMR 7265, 13108 Saint-Paul-lez-Durance, France.|CEA, DSV, IBEB, SBVME, LIPM, 13108 Saint-Paul-lez-Durance, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Berthomieu
authors.affiliation: Université d'Aix-Marseille, 13108 Saint-Paul-lez-Durance, France.|CNRS, UMR 7265, 13108 Saint-Paul-lez-Durance, France.|CEA, DSV, IBEB, SBVME, LIPM, 13108 Saint-Paul-lez-Durance, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Chapon
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005602:France; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D014501:Uranium; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012987:Soil; D024482:Vitamin K 2; D014501:Uranium