title: [Effects of exogenous carnitine on function of respiratory chain and antioxidant capacity in mitochondria of myocardium after exhaustive running in rats].
abstract: OBJECTIVE
  To investigate the effect of exogenous carnitine on function of respiratory chain and antioxidant capacity in mitochondria of myocardium in rats.
  
  
  METHODS
  Forty male Wistar rats were randomized to 4 groups (n = 10): static control (C), supplementation of carnitine (LC), exercise-training (T) and training with supplementation of carnitine (TLC). LC and TLC animals were perfused carnitine by the dose of 300 mg/kg bw x d. T and TLC animals were forced to performed 6-week treadmill training. Heart were prepared immediately after exhaustive running. Myocardium mitochondria was extracted by differential centrifugation. Spectrophotometric analysis was used to evaluate activities of respiratory chain complex (C) I -IV and superoxide dismutase (SOD), malondialdehyde (MDA) level in myocardium mitochondria.
  
  
  RESULTS
  To compare with C group, C I and C IV activity in LC, T and TLC group were increased significantly (P < 0.05, P < 0.01), CII and C III activity in T and TLC group were increased significantly (P < 0.05, P < 0.01); to compare with LC group, C I - IV activity in TLC group were increased significantly (P < 0.05, P < 0.01); to compare with T group, CI and C IV activity in TLC group increased significantly (P < 0.05). To compare with C group, SOD activity increased remarkably, MDA was remarkably lower (P < 0.05, P < 0.01) in LC, T and TLC group; To compare with LC and T group, SOD activity increased remarkably, MDA was remarkably lower (P < 0.05) in TLC group.
  
  
  CONCLUSIONS
  Carnitine and training could improve function of respiratory chain and increased antioxidant capacity in myocardium mitochondria, there was better function of cooperation between carnitine and training.
authors.affiliation: College of Physical Education, Northwest Normal University, Lanzhou 730070, China. lijie2005ty@126.com
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Yu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002331:Carnitine / Q000494:pharmacology*; D004579:Electron Transport; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D002331:Carnitine