title: Development of monoclonal antibodies against the abnormal prion protein isoform (PrP(res)) associated with chronic wasting disease (CWD).
abstract: Monoclonal antibodies (mAbs) specific for the abnormal prion protein isoform (PrP(res)) are indispensable for diagnosing chronic wasting disease (CWD). In this study, eight mAbs were developed by immunizing PrP knockout mice with recombinant elk PrP and an immunogenic PrP peptide. The reactivity of the mAbs to recombinant PrP and the PrP peptide was measured, and their isotypes were subsequently determined. Among them, four mAbs (B85-05, B85-08, B85-12, and B77-75) were shown by Western blotting to recognize proteinase K-treated brain homogenate derived from an elk suffering from CWD.
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Korea.
authors.forename: Hyun-Jeong
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Nak-Hyung
authors.initials: NH
authors.lastname: Lee
authors.forename: Joong-Bok
authors.initials: JB
authors.lastname: Lee
authors.forename: Seung-Yong
authors.initials: SY
authors.lastname: Park
authors.forename: Chang-Seon
authors.initials: CS
authors.lastname: Song
authors.forename: Kun-Ho
authors.initials: KH
authors.lastname: Seo
authors.forename: Dong-Woon
authors.initials: DW
authors.lastname: Kim
authors.forename: Yong-Sun
authors.initials: YS
authors.lastname: Kim
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Choi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics; D015153:Blotting, Western / Q000662:veterinary; D017931:DNA Primers / Q000235:genetics; D003670:Deer / Q000276:immunology* / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D019286:Endopeptidase K; D051379:Mice; D018345:Mice, Knockout; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011328:Prions / Q000235:genetics / Q000276:immunology*; D020033:Protein Isoforms / Q000235:genetics / Q000276:immunology*; D034081:Wasting Disease, Chronic / Q000175:diagnosis* / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D017931:DNA Primers; D011328:Prions; D020033:Protein Isoforms; D019286:Endopeptidase K