title: Magnetic solid-phase extraction and determination of puerarin in rat plasma using C(18)-functionalized magnetic silica nanoparticles by high performance liquid chromatography.
abstract: In the paper, we presented a magnetic solid-phase extraction (MSPE) method based on C(18)-functionalized magnetic silica nanoparticles for the analysis of puerarin in rat plasma. The approach involves two steps including synthesis of magnetic solid-phase sorbents and bioanalysis. The synthesized magnetic silica microspheres modified with chloro(dimethyl)octylsilane (namely Fe(3)O(4)@SiO(2)-C(18)) can provide an efficient way for the extraction of puerarin through C(18) hydrophobic interaction. The puerarin could be easily enriched using milligram-level Fe(3)O(4)@SiO(2)-C(18) sorbents with vibration for 10min. By means of a magnet, puerarin adsorbed with Fe(3)O(4)@SiO(2)-C(18) sorbents was easily isolated from the matrix, and desorbed with CAN. No carryover was observed, and the sorbents could be recycled in our study. The method recoveries were obtained from 85.2% to 92.3%. Limits of quantification and limits of detection of 0.1μgmL(-1) and 0.05μgmL(-1), respectively were achieved. The precision was from 8.1 to 13.7% for intra-day measurement, and from 9.4 to 15.2% for inter-day variation. The accuracy ranged from 94.7 to 106.3% for intra-day measurement, and from 93.3 to 107.8% for inter-day measurement. The MSPE method was applied for analysis of puerarin in rat plasma samples. The results indicated that it was convenient and efficient for the determination of puerarin in biosamples.
authors.affiliation: Emergency Department, Chinese Air Force General Hospital, No. 30 Fucheng Road, Beijing 100142, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Lijie
authors.initials: L
authors.lastname: Huang
authors.forename: Panfeng
authors.initials: P
authors.lastname: Yu
authors.forename: Jianchang
authors.initials: J
authors.lastname: Wang
authors.forename: Shun
authors.initials: S
authors.lastname: Shen
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007529:Isoflavones / Q000097:blood*; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry*; D051381:Rats; D012822:Silicon Dioxide / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D007529:Isoflavones; D012822:Silicon Dioxide; C033607:puerarin