title: Expression of neurexin and neuroligin in the enteric nervous system and their down-regulated expression levels in Hirschsprung disease.
abstract: To investigate the expression levels of neurexins and neuroligins in the enteric nervous system (ENS) in Hirschsprung Disease (HSCR). Longitudinal muscles with adherent mesenteric plexus were obtained by dissection of the fresh gut wall of mice, guinea pigs, and humans. Double labeling of neurexin I and Hu (a neuron marker), neuroligin 1 and Hu, neurexin I and synaptophysin (a presynaptic marker), and neuroligin 1 and PSD95 (a postsynaptic marker) was performed by immunofluorescence staining. Images were merged to determine the relative localizations of the proteins. Expression levels of neurexin and neuroligin in different segments of the ENS in HSCR were investigated by immunohistochemistry. Neurexin and neuroligin were detected in the mesenteric plexus of mice, guinea pigs, and humans with HSCR. Neurexin was located in the presynapse, whereas neuroligin was located in the postsynapse. Expression levels of neurexin and neuroligin were significant in the ganglionic colonic segment of HSCR, moderate in the transitional segment, and negative in the aganglionic colonic segment. The expressions of neurexin and neuroligin in the transitional segments were significantly down-regulated compared with the levels in the normal segments (P < 0.05). Expression levels of neurexin and neuroligin in ENS are significantly down-regulated in HSCR, which may be involved in the pathogenesis of HSCR.
authors.affiliation: Department of Pediatric Surgery, Qilu Hospital, Shandong University, 107 Wenhuaxi Road, Jinan 250012, Shandong, China.
authors.forename: Qiangye
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Aiwu
authors.initials: A
authors.lastname: Li
authors.forename: Hongzhen
authors.initials: H
authors.lastname: Liu
authors.forename: Wentong
authors.initials: W
authors.lastname: Zhang
authors.forename: Xinhai
authors.initials: X
authors.lastname: Cui
authors.forename: Kelai
authors.initials: K
authors.lastname: Wang
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism*; D015536:Down-Regulation; D051959:ELAV Proteins / Q000378:metabolism; D017615:Enteric Nervous System / Q000378:metabolism* / Q000473:pathology; D006168:Guinea Pigs; D006627:Hirschsprung Disease / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008531:Megacolon / Q000378:metabolism / Q000473:pathology; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D019006:Neural Cell Adhesion Molecules; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D013569:Synapses / Q000378:metabolism; D016708:Synaptophysin
chemical_list: D002135:Calcium-Binding Proteins; D015816:Cell Adhesion Molecules, Neuronal; D051959:ELAV Proteins; C539628:NRXN1 protein, human; D009419:Nerve Tissue Proteins; D019006:Neural Cell Adhesion Molecules; D016708:Synaptophysin; C093438:neuroligin 1