title: The SNP in the promoter region of the bovine ELOVL5 gene influences economic traits including subcutaneous fat thickness.
abstract: Genetic analyses have contributed to improvements of economically important traits derived from adipose tissue such as fatty acid composition in beef. Elongation of very long chain fatty acids (ELOVL) genes encode for the enzymes that play an important role in elongation of long-chain fatty acids. In this study, we aimed to discover genetic polymorphisms of ELOVL gene family in cattle populations to develop genetic markers. As a result, five synonymous mutations were detected in the coding regions of the ELOVL1, ELOVL2, ELOVL3 and ELOVL5 genes. In addition, six mutations were identified in promoter region of the ELOVL5. Two of five mutations in the promoter region of ELOVL5 were expected to alter the ELOVL5 expression and influence the economic traits, because of the high synteny of the region which was essential for activation of Elovl5 in mouse. Therefore, we performed association analysis between the genotypes and traits and our result revealed that T allele of g.-110T>C in ELOVL5 gene promoter indicated significantly thinner subcutaneous fat thickness (TT, 2.39 cm; CT, 2.35 cm) than that of C allele (CC, 2.68 cm) in a Japanese Black population. Our results suggest that the g.-110T>C is a useful genetic marker for the breeding in beef cattle.
authors.affiliation: Laboratory of Animal Breeding and Genetics, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Matsumoto
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Shimizu
authors.forename: Atsuko
authors.initials: A
authors.lastname: Tanaka
authors.forename: Takuya
authors.initials: T
authors.lastname: Nogi
authors.forename: Ichiro
authors.initials: I
authors.lastname: Tabuchi
authors.forename: Kenji
authors.initials: K
authors.lastname: Oyama
authors.forename: Masaaki
authors.initials: M
authors.lastname: Taniguchi
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Mannen
authors.forename: Shinji
authors.initials: S
authors.lastname: Sasazaki
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D000818:Animals; D002417:Cattle; D000081031:Fatty Acid Elongases; D056726:Genetic Association Studies; D009154:Mutation; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics; D050151:Subcutaneous Fat / Q000254:growth & development*; D026801:Synteny
chemical_list: D000123:Acetyltransferases; C581235:Elovl5 protein, mouse; D000081031:Fatty Acid Elongases