title: Overlapping glycosylation sequon influences the glycosylation pattern of a chimeric protein expressed in tomato leaf and callus.
abstract: Overlapping glycosylation sequon (OGS) is composed of two overlapping N-glycosylation sequons and has been found in certain glycoproteins with pharmaceutical value. With a growing interest to produce pharmaceutical glycoproteins in plants, it is important to establish the glycosylation pattern of OGS-containing proteins expressed in varying plant tissues. Here, a chimeric OGS (NNST)-containing gene and its mutated forms (NNAT and NASNAT) were expressed in callus-derived tomato plantlets. Tissue extracts from the recombinant leaf and callus were used in immunoblotting and glycoprotein detection. We found that the glycosylation patterns of the NNST-containing chimeric protein differ from that of NNAT- and NASNAT-containing proteins.
authors.affiliation: Department of Molecular and Cellular Biology, Nagoya City University, Aichi 467-8601, Japan. marni.cueno@nihon-u.ac.jp
authors.forename: Marni E
authors.initials: ME
authors.lastname: Cueno
authors.forename: Kenichi
authors.initials: K
authors.lastname: Imai
authors.forename: Takashi
authors.initials: T
authors.lastname: Okamoto
authors.forename: Kuniyasu
authors.initials: K
authors.lastname: Ochiai
mesh_terms: D000595:Amino Acid Sequence; D002478:Cells, Cultured; D006031:Glycosylation; D018551:Solanum lycopersicum / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000378:metabolism*; D010940:Plant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D010940:Plant Proteins