title: A host-specific biological control of grape crown gall by Agrobacterium vitis strain F2/5: its regulation and population dynamics.
abstract: Nontumorigenic Agrobacterium vitis strain F2/5 is able to prevent crown gall caused by tumorigenic A. vitis on grape but not on other plant species such as tobacco. Mutations in a quorum-sensing transcription factor, aviR, and in caseinolytic protease (clp) component genes clpA and clpP1 resulted in reduced or loss of biological control. All mutants were complemented; however, restoration of biological control by complemented clpA and clpP1 mutants was dependent on the copy number of vector that was used as well as timing of application of the complemented mutants to grape wounds in relation to inoculation with pathogen. Mutations in other quorum-sensing and clp genes and in a gene associated with polyketide synthesis did not affect biological control. It was determined that, although F2/5 inhibits transformation by tumorigenic A. vitis strains on grape, it does not affect growth of the pathogen in wounded grape tissue over time.
authors.affiliation: Department of Plant Pathology and Plant Microbe Biology, Cornell University, NY, USA.
authors.forename: Supaporn
authors.initials: S
authors.lastname: Kaewnum
authors.forename: Desen
authors.initials: D
authors.lastname: Zheng
authors.forename: Cheryl L
authors.initials: CL
authors.lastname: Reid
authors.forename: Kameka L
authors.initials: KL
authors.lastname: Johnson
authors.forename: Jodi C
authors.initials: JC
authors.lastname: Gee
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Burr
mesh_terms: D060054:Agrobacterium / Q000254:growth & development / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics*; D061046:Biological Control Agents; D015169:Colony Count, Microbial; D003710:Demography; D049071:Endopeptidase Clp / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D005816:Genetic Complementation Test; D058507:Host Specificity; D054884:Host-Pathogen Interactions; D056265:Microbial Interactions; D050296:Microbial Viability; D010941:Plant Tumors / Q000382:microbiology*; D053038:Quorum Sensing; D017384:Sequence Deletion; D013045:Species Specificity; D013997:Time Factors; D014026:Nicotiana / Q000382:microbiology; D014157:Transcription Factors / Q000235:genetics; D027843:Vitis / Q000382:microbiology*; D014947:Wounds and Injuries
chemical_list: D001426:Bacterial Proteins; D061046:Biological Control Agents; D014157:Transcription Factors; D049071:Endopeptidase Clp