title: The Drosophila Z-disc protein Z(210) is an adult muscle isoform of Zasp52, which is required for normal myofibril organization in indirect flight muscles.
abstract: The Z-disc is a critical anchoring point for thin filaments as they slide during muscle contraction. Therefore, identifying components of the Z-disc is critical for fully comprehending how myofibrils assemble and function. In the adult Drosophila musculature, the fibrillar indirect flight muscles accumulate a >200 kDa Z-disc protein termed Z(210), the identity of which has to date been unknown. Here, we use mass spectrometry and gene specific knockdown studies, to identify Z(210) as an adult isoform of the Z-disc protein Zasp52. The Zasp52 primary transcript is extensively alternatively spliced, and we describe its splicing pattern in the flight muscles, identifying a new Zasp52 isoform, which is the one recognized by the Z(210) antibody. We also demonstrate that Zasp52 is required for the association of α-actinin with the flight muscle Z-disc, and for normal sarcomere structure. These studies expand our knowledge of Zasp isoforms and their functions in muscle. Given the role of Zasp proteins in mammalian muscle development and disease, our results have relevance to mammalian muscle biology.
authors.affiliation: Department of Biology, University of New Mexico, Albuquerque, New Mexico 87131, USA.
authors.forename: Maria B
authors.initials: MB
authors.lastname: Chechenova
authors.forename: Anton L
authors.initials: AL
authors.lastname: Bryantsev
authors.forename: Richard M
authors.initials: RM
authors.lastname: Cripps
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism*; D017398:Alternative Splicing / Q000502:physiology*; D000818:Animals; D002352:Carrier Proteins; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster; D055785:Gene Knockdown Techniques; D060588:LIM Domain Proteins / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012518:Sarcomeres / Q000235:genetics / Q000378:metabolism*
chemical_list: D000199:Actins; D002352:Carrier Proteins; D029721:Drosophila Proteins; D060588:LIM Domain Proteins; D020033:Protein Isoforms; C526450:Zasp52 protein, Drosophila