title: Urinary metabolite concentrations of phthalate metabolites in Central Italy healthy volunteers determined by a validated HPLC/MS/MS analytical method.
abstract: The main objective of this study was to determine the average concentrations for the metabolites of the four more common phthalates, industrial chemicals widely used in commercial products and potential endocrine disruptors, in the urine of a control population living in Central Italy. The study population consisted of 157 healthy subjects, not occupationally exposed to phthalates (74 males and 83 females). Urinary levels of the analytes were determined by liquid chromatography tandem mass spectrometry (LC-MS/MS) with isotopic dilution. The reference values (geometric mean) for males and females were estimated for each metabolite:. Females: for MEP was 72.94μg/g creatinine (CI 95% 3.63-149.51), for MEHP was 3.37μg/g creatinine (CI 95% 1.85-4.89), for MEHHP was 12.74μg/g creatinine (CI 95% 9.45-22.19), for MnBP was 20.26μg/g creatinine (CI 95% 8.17-28.43) and for MBzP was 14.74 (CI 95% 2.94-17.68). Males: for MEP was 56.35μg/g creatinine (CI 95% 2.32-110.39), for MEHP was 2.80μg/g creatinine (CI 95% 1.08-4.52), for MEHHP was 10.77μg/g creatinine (CI 95% 6.18-16.95), for MnBP was 17.59μg/g creatinine (CI 95% 5.72-29.45) and for MBzP was 16.44 (CI 95% 7.90-29.45). To obtain reference values for these chemicals is without doubt an important topic for evaluate the exposure of population and their possible health effects. Information from different geographical areas are important to understand the real different background concentrations.
authors.affiliation: INAIL-Research, Certification, Verification Area, Department of Occupational Medicine, Research Center of Monteporzio Catone, Via di Fontana Candida 1, 00040 Rome, Italy. g.tranfo@inail.it
authors.forename: Giovanna
authors.initials: G
authors.lastname: Tranfo
authors.forename: Bruno
authors.initials: B
authors.lastname: Papaleo
authors.forename: Lidia
authors.initials: L
authors.lastname: Caporossi
authors.forename: Silvia
authors.initials: S
authors.lastname: Capanna
authors.forename: Mariangela
authors.initials: M
authors.lastname: De Rosa
authors.forename: Daniela
authors.initials: D
authors.lastname: Pigini
authors.forename: Federica
authors.initials: F
authors.lastname: Corsetti
authors.forename: Enrico
authors.initials: E
authors.lastname: Paci
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004784:Environmental Monitoring; D004785:Environmental Pollutants / Q000652:urine*; D005260:Female; D064368:Healthy Volunteers / Q000706:statistics & numerical data; D006801:Humans; D007558:Italy; D008297:Male; D008875:Middle Aged; D010795:Phthalic Acids / Q000652:urine*; D012016:Reference Values; D053719:Tandem Mass Spectrometry; D055815:Young Adult
chemical_list: D004785:Environmental Pollutants; D010795:Phthalic Acids; C032279:phthalic acid