title: HyPer-3: a genetically encoded H(2)O(2) probe with improved performance for ratiometric and fluorescence lifetime imaging.
abstract: High-performance sensors for reactive oxygen species are instrumental to monitor dynamic events in cells and organisms. Here, we present HyPer-3, a genetically encoded fluorescent indicator for intracellular H2O2 exhibiting improved performance with respect to response time and speed. HyPer-3 has an expanded dynamic range compared to HyPer and significantly faster oxidation/reduction dynamics compared to HyPer-2. We demonstrate this performance by in vivo imaging of tissue-scale H2O2 gradients in zebrafish larvae. Moreover, HyPer-3 was successfully employed for single-wavelength fluorescent lifetime imaging of H2O2 levels both in vitro and in vivo.
authors.affiliation: Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
authors.forename: Dmitry S
authors.initials: DS
authors.lastname: Bilan
authors.forename: Luke
authors.initials: L
authors.lastname: Pase
authors.forename: Linda
authors.initials: L
authors.lastname: Joosen
authors.forename: Andrey Yu
authors.initials: AY
authors.lastname: Gorokhovatsky
authors.forename: Yulia G
authors.initials: YG
authors.lastname: Ermakova
authors.forename: Theodorus W J
authors.initials: TW
authors.lastname: Gadella
authors.forename: Clemens
authors.initials: C
authors.lastname: Grabher
authors.forename: Carsten
authors.initials: C
authors.lastname: Schultz
authors.forename: Sergey
authors.initials: S
authors.lastname: Lukyanov
authors.forename: Vsevolod V
authors.initials: VV
authors.lastname: Belousov
mesh_terms: D000818:Animals; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis*; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000378:metabolism; D007814:Larva / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods*; D010084:Oxidation-Reduction; D015027:Zebrafish / Q000254:growth & development / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D006861:Hydrogen Peroxide