title: Serotype analysis of Streptococcus pneumoniae in lung and nasopharyngeal aspirates from children in the Gambia by MassTag PCR.
abstract: Streptococcus pneumoniae strains comprise >90 serotypes. Here we describe establishment of a MassTag PCR assay designed to serotype S. pneumoniae and demonstrate its utility in tests using 31 paired lung aspirate and nasopharyngeal aspirate samples from children with pneumonia in the Gambia. Serotypes 1, 5, and 14 in were implicated in 90% of lung infections. With 5 exceptions, serotypes found in lung aspirates were also found in nasopharyngeal aspirates.
authors.affiliation: Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA. rt2249@columbia.edu
authors.forename: Rafal
authors.initials: R
authors.lastname: Tokarz
authors.forename: Thomas
authors.initials: T
authors.lastname: Briese
authors.forename: Gerard
authors.initials: G
authors.lastname: Morris
authors.forename: Readon
authors.initials: R
authors.lastname: Ideh
authors.forename: Osaretin
authors.initials: O
authors.lastname: Chimah
authors.forename: Bernard
authors.initials: B
authors.lastname: Ebruke
authors.forename: Aaloki
authors.initials: A
authors.lastname: Desai
authors.forename: Saddef
authors.initials: S
authors.lastname: Haq
authors.forename: Stephen
authors.initials: S
authors.lastname: Sameroff
authors.forename: Stephen R C
authors.initials: SR
authors.lastname: Howie
authors.forename: W Ian
authors.initials: WI
authors.lastname: Lipkin
mesh_terms: D002675:Child, Preschool; D005714:Gambia / Q000453:epidemiology; D006801:Humans; D007223:Infant; D008168:Lung / Q000382:microbiology*; D058889:Molecular Typing; D009305:Nasopharynx / Q000382:microbiology*; D011018:Pneumonia, Pneumococcal / Q000453:epidemiology* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000302:isolation & purification