title: Antigen-specific regulation of IgE antibodies by non-antigen-specific γδ T cells.
abstract: We re-examined the observation that γδ T cells, when transferred from mice tolerized to an inhaled conventional Ag, suppress the allergic IgE response to this Ag specifically. Using OVA and hen egg lysozyme in crisscross fashion, we confirmed the Ag-specific IgE-regulatory effect of the γδ T cells. Although only Vγ4(+) γδ T cells are regulators, the Ag specificity does not stem from specificity of their γδ TCRs. Instead, the Vγ4(+) γδ T cells failed to respond to either Ag, but rapidly acquired Ag-specific regulatory function in vivo following i.v. injection of non-T cells derived from the spleen of Ag-tolerized mice. This correlated with their in vivo Ag acquisition from i.v. injected Ag-loaded splenic non-T cells, and in vivo transfer of membrane label provided evidence for direct contact between the injected splenic non-T cells and the Vγ4(+) γδ T cells. Together, our data suggest that Ag itself, when acquired by γδ T cells, directs the specificity of their IgE suppression.
authors.affiliation: Integrated Department of Immunology, National Jewish Health, Denver, CO 80206, USA.
authors.forename: Yafei
authors.initials: Y
authors.lastname: Huang
authors.forename: M Kemal
authors.initials: MK
authors.lastname: Aydintug
authors.forename: Joshua
authors.initials: J
authors.lastname: Loomis
authors.forename: Megan K
authors.initials: MK
authors.lastname: Macleod
authors.forename: Amy S
authors.initials: AS
authors.lastname: McKee
authors.forename: Greg
authors.initials: G
authors.lastname: Kirchenbaum
authors.forename: Claudia V
authors.initials: CV
authors.lastname: Jakubzick
authors.forename: Ross M
authors.initials: RM
authors.lastname: Kedl
authors.forename: Deming
authors.initials: D
authors.lastname: Sun
authors.forename: Jordan
authors.initials: J
authors.lastname: Jacobelli
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: O'Brien
authors.forename: Willi K
authors.initials: WK
authors.lastname: Born
mesh_terms: D000280:Administration, Inhalation; D019264:Adoptive Transfer; D000336:Aerosols; D000818:Animals; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology* / Q000633:toxicity; D001249:Asthma / Q000209:etiology / Q000276:immunology*; D002469:Cell Separation; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D007073:Immunoglobulin E / Q000276:immunology*; D054992:Immunological Synapses; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D009113:Muramidase / Q000008:administration & dosage / Q000276:immunology* / Q000633:toxicity; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology* / Q000633:toxicity; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D013154:Spleen / Q000276:immunology; D037182:T-Cell Antigen Receptor Specificity; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000637:transplantation
chemical_list: D000336:Aerosols; D000941:Antigens; D016692:Receptors, Antigen, T-Cell, gamma-delta; D007073:Immunoglobulin E; D010047:Ovalbumin; C421864:hen egg lysozyme; D009113:Muramidase