title: Current trends in separation of plasmid DNA vaccines: a review.
abstract: Plasmid DNA (pDNA)-based vaccines offer more rapid avenues for development and production if compared to those of conventional virus-based vaccines. They do not rely on time- or labour-intensive cell culture processes and allow greater flexibility in shipping and storage. Stimulating antibodies and cell-mediated components of the immune system are considered as some of the major advantages associated with the use of pDNA vaccines. This review summarizes the current trends in the purification of pDNA vaccines for practical and analytical applications. Special attention is paid to chromatographic techniques aimed at reducing the steps of final purification, post primary isolation and intermediate recovery, in order to reduce the number of steps necessary to reach a purified end product from the crude plasmid.
authors.affiliation: Faculty of Applied Science, University of Canberra, Australian Capital Territory (ACT) 2617, Australia. ashraf.ghanem@canberra.edu.au
authors.forename: Ashraf
authors.initials: A
authors.lastname: Ghanem
authors.forename: Robert
authors.initials: R
authors.lastname: Healey
authors.forename: Frady G
authors.initials: FG
authors.lastname: Adly
mesh_terms: D002846:Chromatography, Affinity / Q000639:trends; D002850:Chromatography, Gel / Q000639:trends; D002851:Chromatography, High Pressure Liquid / Q000639:trends; D002852:Chromatography, Ion Exchange / Q000639:trends; D056148:Chromatography, Reverse-Phase / Q000639:trends; D004278:DNA, Superhelical / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D012492:Salts / Q000737:chemistry; D019444:Vaccines, DNA / Q000276:immunology / Q000302:isolation & purification*
chemical_list: D004278:DNA, Superhelical; D012492:Salts; D019444:Vaccines, DNA