title: Simultaneous detection of flavonoids and phenolic acids in Herba Lysimachiae and Herba Desmodii Styracifolii using liquid chromatography tandem mass spectrometry.
abstract: A sensitive liquid chromatography and tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous analysis of 15 flavonoids and 3 phenolic acids in Herba Lysimachiae and Herba Desmodii Styracifolii. Separation was performed using a Diamonsil C(18) column, which was eluted with methanol (A) and 0.1‰ acetic acid (B). The gradient condition was as follows: 0-34 min, 20-34% A; 34-38 min, 34-95% A; maintained 95% A for the next 4 min. Analytes were detected using a hybrid quadrupole linear ion trap mass spectrometer that was equipped with an electrospray ionisation source in the negative ion and multiple-reaction monitoring modes. A full validation of the method was performed, including the linearity, precision, and accuracy, as well as limits of detection and quantification. The results indicated that the developed method was simple, sensitive and reliable. Furthermore, the method was successfully applied to differentiate 16 batches of Herba Lysimachiae and 21 batches of Herba Desmodii Styracifolii.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, PR China.
authors.forename: Dongxiao
authors.initials: D
authors.lastname: Sun
authors.forename: Lihua
authors.initials: L
authors.lastname: Dong
authors.forename: Panpan
authors.initials: P
authors.lastname: Guo
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Shi
authors.forename: Jia
authors.initials: J
authors.lastname: Gao
authors.forename: Yanping
authors.initials: Y
authors.lastname: Ren
authors.forename: Xijuan
authors.initials: X
authors.lastname: Jiang
authors.forename: Wenxin
authors.initials: W
authors.lastname: Li
authors.forename: Chunying
authors.initials: C
authors.lastname: Wang
authors.forename: Qiao
authors.initials: Q
authors.lastname: Wang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007887:Fabaceae / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry*; D062385:Hydroxybenzoates / Q000737:chemistry*; D029623:Primulaceae / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D062385:Hydroxybenzoates; C017616:phenolic acid