title: Mycobacterium fragae sp. nov., a non-chromogenic species isolated from human respiratory specimens.
abstract: Three isolates of a slow-growing, non-chromogenic mycobacterium were grown from three sputum samples of a patient from the north-eastern Ceará state in Brazil. Identification at species level could not be obtained with PCR restriction analysis of the hsp65 gene. In order to characterize the isolates we carried out phenotypic and genotypic tests. We sequenced the nearly complete 16S rRNA gene and obtained partial sequences of the hsp65 (encoding the hypervariable region of the 65 kDa heat-shock protein) and rpoB (encoding the beta-subunit of RNA polymerase) genes. The three isolates turned out to be identical and most closely related to the species Mycobacterium celatum and Mycobacterium kyorinense. The results, however, showed significant differences between these species and the isolates studied, which led us to consider them members of a novel species for which we propose the name Mycobacterium fragae. The type strain is HF8705(T) ( = Fiocruz-INCQS/CMRVS P4051(T) = DSM 45731(T)).
authors.affiliation: National Reference Laboratory for Tuberculosis, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, Fiocruz - RJ, Brazil.
authors.forename: Jesus Pais
authors.initials: JP
authors.lastname: Ramos
authors.affiliation: National Reference Laboratory for Tuberculosis, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, Fiocruz - RJ, Brazil.
authors.forename: Carlos Eduardo Dias
authors.initials: CED
authors.lastname: Campos
authors.affiliation: National Reference Laboratory for Tuberculosis, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, Fiocruz - RJ, Brazil.
authors.forename: Paulo Cesar de Souza
authors.initials: PCS
authors.lastname: Caldas
authors.affiliation: National Reference Laboratory for Tuberculosis, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, Fiocruz - RJ, Brazil.
authors.forename: Nicole Victor
authors.initials: NV
authors.lastname: Ferreira
authors.affiliation: National Reference Laboratory for Tuberculosis, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, Fiocruz - RJ, Brazil.
authors.forename: Mariza Villas
authors.initials: MV
authors.lastname: Boas da Silva
authors.affiliation: National Reference Laboratory for Tuberculosis, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, Fiocruz - RJ, Brazil.
authors.forename: Paulo
authors.initials: P
authors.lastname: Redner
authors.affiliation: Mycobacteria Sector, Central Laboratory of Ceará State, Brazil (LACEN-CE).
authors.forename: Creusa Lima
authors.initials: CL
authors.lastname: Campelo
authors.affiliation: Mycobacteria Sector, Central Laboratory of Ceará State, Brazil (LACEN-CE).
authors.forename: Sheila Ferreira
authors.initials: SF
authors.lastname: Vale
authors.affiliation: Ambulatório de micobacterioses, Hospital Carlos Alberto Studart Gomes, Health Secretary of Ceará, Brazil.
authors.forename: Elizabeth Clara
authors.initials: EC
authors.lastname: Barroso
authors.affiliation: National Reference Laboratory for Tuberculosis, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, Fiocruz - RJ, Brazil.
authors.forename: Reginalda Ferreira de Melo
authors.initials: RFM
authors.lastname: Medeiros
authors.affiliation: National Reference Laboratory for Tuberculosis, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, Fiocruz - RJ, Brazil.
authors.forename: Fátima Cristina Onofre Fandinho
authors.initials: FCOF
authors.lastname: Montes
authors.affiliation: National Reference Laboratory for Tuberculosis, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, Fiocruz - RJ, Brazil.
authors.forename: Teca Calcagno
authors.initials: TC
authors.lastname: Galvão
authors.affiliation: Emerging Bacterial Pathogens Unit, San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Tortoli
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001938:Brazil; D018834:Chaperonin 60 / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D008171:Lung Diseases / Q000382:microbiology; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009164:Mycobacterium Infections / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013183:Sputum / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; C074119:heat-shock protein 65, Mycobacterium