title: Expression analysis of mir-17-5p, mir-20a and let-7a microRNAs and their target proteins in CD34+ bone marrow cells of patients with myelodysplastic syndromes.
abstract: Mir-17-5p and mir-20a, members of the mir-17-92 family, down-regulate E2F1, which is over-expressed in myelodysplastic syndromes (MDS). Moreover, let-7a down-regulates KRAS, which is aberrantly expressed in MDS. We evaluated the expression of the aforementioned microRNAs in CD34+ cells of 43 MDS patients using real-time PCR and their target proteins (E2F1, MYC, BCL2, CCND1, and KRAS) by Western blot. Mir-17-5p and mir-20a were under expressed in high risk MDS patients, compared to low risk MDS patients. Similarly, let-7a was under expressed in patients with intermediate or high-risk karyotype. Interestingly, there was an inverse correlation between microRNA and the expression levels of their targets. Importantly, mir-17-5p and mir-20a constitute favorable prognostic factors in MDS, since their expression was associated with increased overall survival of MDS patients.
authors.affiliation: Second Department of Internal Medicine and Research Institute, Athens University Medical School, "Attikon" University General Hospital, Athens, Greece.
authors.forename: Diamantina
authors.initials: D
authors.lastname: Vasilatou
authors.forename: Sotirios G
authors.initials: SG
authors.lastname: Papageorgiou
authors.forename: Frieda
authors.initials: F
authors.lastname: Kontsioti
authors.forename: Christos K
authors.initials: CK
authors.lastname: Kontos
authors.forename: Panayiota
authors.initials: P
authors.lastname: Tsiotra
authors.forename: Vassiliki
authors.initials: V
authors.lastname: Mpakou
authors.forename: Maria-Angeliki S
authors.initials: MA
authors.lastname: Pavlou
authors.forename: Christina
authors.initials: C
authors.lastname: Economopoulou
authors.forename: George
authors.initials: G
authors.lastname: Dimitriadis
authors.forename: John
authors.initials: J
authors.lastname: Dervenoulas
authors.forename: Vasiliki
authors.initials: V
authors.lastname: Pappa
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D018952:Antigens, CD34 / Q000378:metabolism; D001854:Bone Marrow Cells / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism / Q000401:mortality; D011379:Prognosis
chemical_list: D018952:Antigens, CD34; C524941:MIRN17 microRNA, human; C534451:MIRN20a microRNA, human; D035683:MicroRNAs; C487931:mirnlet7 microRNA, human