title: Dendritic cell activation by polysaccharide isolated from Angelica dahurica.
abstract: Angelica dahurica is used in functional foods for the prevention and treatment of various diseases, such as inflammation and cancer. In the present study, we examined the effect of A. dahurica polysaccharide (ADP) on dendritic cell (DC) maturation. ADP increased the expressions of CD86 and MHC-II molecules, the production of IL-12, IL-1β, and TNF-α, and allogeneic T cell activation ability of DCs, and reduced DC endocytosis. As a mechanism of action, the knockdown of TLR4 with small interfering RNA decreased the ADP-induced production of nitric oxide and IL-12 by DCs, suggesting the membrane receptor candidate of ADP. After binding to TLR4, ADP increased the phosphorylation of ERK, JNK, and p38 MAPKs, and the nuclear translocation of NF-κB p50/p65. These results indicate that ADP activates DCs through TLR4 and downstream signalings.
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.
authors.forename: Hyung Sook
authors.initials: HS
authors.lastname: Kim
authors.forename: Bo Ram
authors.initials: BR
authors.lastname: Shin
authors.forename: Hong Kyung
authors.initials: HK
authors.lastname: Lee
authors.forename: Yun Soo
authors.initials: YS
authors.lastname: Park
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: Sung Yeon
authors.initials: SY
authors.lastname: Kim
authors.forename: Mi Kyeong
authors.initials: MK
authors.lastname: Lee
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
authors.forename: Youngsoo
authors.initials: Y
authors.lastname: Kim
authors.forename: Sang-Bae
authors.initials: SB
authors.lastname: Han
mesh_terms: D029969:Angelica / Q000737:chemistry*; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology; D004789:Enzyme Activation; D005260:Female; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011134:Polysaccharides / Q000494:pharmacology*
chemical_list: D017931:DNA Primers; D011134:Polysaccharides; D020928:Mitogen-Activated Protein Kinases