title: Cloning and expression of feline colony stimulating factor receptor (CSF-1R) and analysis of the species specificity of stimulation by colony stimulating factor-1 (CSF-1) and interleukin-34 (IL-34).
abstract: Colony stimulating factor (CSF-1) and its receptor, CSF-1R, have been previously well studied in humans and rodents to dissect the role they play in development of cells of the mononuclear phagocyte system. A second ligand for the CSF-1R, IL-34 has been described in several species. In this study, we have cloned and expressed the feline CSF-1R and examined the responsiveness to CSF-1 and IL-34 from a range of species. The results indicate that pig and human CSF-1 and human IL-34 are equally effective in cats, where both mouse CSF-1 and IL-34 are significantly less active. Recombinant human CSF-1 can be used to generate populations of feline bone marrow and monocyte derived macrophages that can be used to further dissect macrophage-specific gene expression in this species, and to compare it to data derived from mouse, human and pig. These results set the scene for therapeutic use of CSF-1 and IL-34 in cats.
authors.affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian EH25 9RG Scotland, UK.
authors.forename: Deborah J
authors.initials: DJ
authors.lastname: Gow
authors.forename: Valerie
authors.initials: V
authors.lastname: Garceau
authors.forename: Clare
authors.initials: C
authors.lastname: Pridans
authors.forename: Adam G
authors.initials: AG
authors.lastname: Gow
authors.forename: Kerry E
authors.initials: KE
authors.lastname: Simpson
authors.forename: Danielle
authors.initials: D
authors.lastname: Gunn-Moore
authors.forename: David A
authors.initials: DA
authors.lastname: Hume
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002415:Cats; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D007378:Interleukins / Q000494:pharmacology*; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010587:Phagocytosis / Q000187:drug effects; D016184:Receptors, Colony-Stimulating Factor / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D013045:Species Specificity; D034421:Sus scrofa
chemical_list: D018076:DNA, Complementary; C529076:IL34 protein, human; D007378:Interleukins; D016184:Receptors, Colony-Stimulating Factor; D011994:Recombinant Proteins; D016173:Macrophage Colony-Stimulating Factor