title: Role of pesticides in the induction of tumor angiogenesis.
abstract: Due to their estrogen-mimicking ability, pesticides are considered as prime etiological suspects of increasing tumor incidence, although a direct link is still undefined. The present study aimed to identify the effect of xenoestrogens (lindane, propoxur and endosulfan) at 20 mg/l each on tumorigenesis, by evaluating endothelial cell proliferation, H(3) thymidine incorporation, wound healing, ascites formation and secretion, shell less Chorio Allantoic Membrane (CAM) formation using in vitro, as well as in vivo, models. The genotoxic effect of xenoestrogens in terms of DNA damage was also studied. The results showed that the endothelial cell proliferation, H(3) thymidine incorporation, wound healing, CAM formation were increased following xenoestrogen exposure, but the intensity of angiogenesis was dependent on the structural homology of these xenoestrogens to endogenous estrogen. Moreover, lindane was the most potent angiogenesis stimulator followed by propoxur and Endosulfan. Further studies were undertaken to examine lindane for its possible carcinogenicity. However, no effect was observed on the integrity of DNA after exposure to these xenoestrogens.
authors.affiliation: Room no. 237, Environmental Biochemistry and Molecular Biology Laboratory, Department of Biochemistry, University College of Medical Sciences (University of Delhi) and GTB Hospital, Dilshad Garden, New Delhi-110095, India.
authors.forename: Salimath P
authors.initials: SP
authors.lastname: Bharathi
authors.forename: Harsh M
authors.initials: HM
authors.lastname: Raj
authors.forename: Smita
authors.initials: S
authors.lastname: Jain
authors.forename: Basu Dev
authors.initials: BD
authors.lastname: Banerjee
authors.forename: Tanzeel
authors.initials: T
authors.lastname: Ahmed
authors.forename: Vinod Kumar
authors.initials: VK
authors.lastname: Arora
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced* / Q000473:pathology; D049033:Chorioallantoic Membrane / Q000187:drug effects / Q000254:growth & development; D004249:DNA Damage / Q000187:drug effects; D004726:Endosulfan / Q000633:toxicity; D042783:Endothelial Cells / Q000187:drug effects; D005260:Female; D001556:Hexachlorocyclohexane / Q000633:toxicity; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D015674:Mammary Neoplasms, Animal / Q000139:chemically induced / Q000473:pathology; D051379:Mice; D009389:Neovascularization, Pathologic / Q000139:chemically induced* / Q000473:pathology; D010575:Pesticides / Q000633:toxicity*; D001074:Propoxur / Q000633:toxicity; D042461:Vascular Endothelial Growth Factor A / Q000008:administration & dosage
chemical_list: D010575:Pesticides; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse; D001556:Hexachlorocyclohexane; D001074:Propoxur; D004726:Endosulfan