title: Y-39983 downregulates RhoA/Rho-associated kinase expression during its             promotion of axonal regeneration.
abstract: Y-39983, a selective Rho-associated kinase (ROCK) inhibitor, promotes axonal             regeneration of damaged retinal ganglion cells (RGCs). The present study investigated             the effects of Y-39983 on RhoA/ROCK expression during promotion of axonal regeneration             using a rat optic nerve crush (ONC) model. Herein, we demonstrated that Y-39983             significantly enhanced the survival and axonal regeneration of RGCs after ONC.             Using a pull‑down assay and affinity precipitation to examine the activity of             RhoA, we detected the decreased expression of active-RhoA after using Y-39983.             The expression of ROCK1 and ROCK2 was significantly decreased as demonstrated             by RT-PCR, immunohistochemistry and western blot analysis. The downregulation             of active-RhoA, ROCK1 and ROCK2 expression by Y-39983 coincided with the appearance             of larger numbers of regenerating axons. In conclusion, Y-39983 downregulated             the expression of active-RhoA, ROCK1 and ROCK2 during its promotion of axonal             regeneration.
authors.affiliation: Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, Shanghai 200025, PR China.
authors.forename: Zijian
authors.initials: Z
authors.lastname: Yang
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Cheng
authors.forename: Lianfu
authors.initials: L
authors.lastname: Deng
authors.forename: Yisheng
authors.initials: Y
authors.lastname: Zhong
mesh_terms: D000818:Animals; D001369:Axons / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects; D015536:Down-Regulation; D004794:Enzyme Repression / Q000187:drug effects; D019922:GAP-43 Protein / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D056965:Injections, Intraocular; D008297:Male; D009416:Nerve Regeneration / Q000187:drug effects; D020221:Optic Nerve Injuries / Q000188:drug therapy* / Q000503:physiopathology; D011725:Pyridines / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012165:Retinal Ganglion Cells / Q000187:drug effects / Q000502:physiology*; D054460:rho-Associated Kinases / Q000235:genetics / Q000378:metabolism*; D020742:rhoA GTP-Binding Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D019922:GAP-43 Protein; D011725:Pyridines; C520365:Y-39983; C578418:ROCK1 protein, rat; C580818:ROCK2 protein, rat; D054460:rho-Associated Kinases; D020742:rhoA GTP-Binding Protein