title: Identification of one novel candidate probiotic Lactobacillus plantarum strain active against influenza virus infection in mice by a large-scale screening.
abstract: In this study, we developed a large-scale screening of bacterial strains in order to identify novel candidate probiotics with immunomodulatory properties. For this, 158 strains, including a majority of lactic acid bacteria (LAB), were screened by two different cellular models: tumor necrosis factor alpha (TNF-α)-activated HT-29 cells and peripheral blood mononuclear cells (PBMCs). Different strains responsive to both models (pro- and anti-inflammatory strains) were selected, and their protective effects were tested in vivo in a murine model of influenza virus infection. Daily intragastric administrations during 10 days before and 10 days after viral challenge (100 PFU of influenza virus H1N1 strain A Puerto Rico/8/1934 [A/PR8/34]/mouse) of Lactobacillus plantarum CNRZ1997, one potentially proinflammatory probiotic strain, led to a significant improvement in mouse health by reducing weight loss, alleviating clinical symptoms, and inhibiting significantly virus proliferation in lungs. In conclusion, in this study, we have combined two cellular models to allow the screening of a large number of LAB for their immunomodulatory properties. Moreover, we identified a novel candidate probiotic strain, L. plantarum CNRZ1997, active against influenza virus infection in mice.
authors.affiliation: INCommensal and Probiotics-Host Interactions Laboratory, Micalis, Jouy-en-Josas, France.
authors.forename: Noura
authors.initials: N
authors.lastname: Kechaou
authors.forename: Florian
authors.initials: F
authors.lastname: Chain
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Gratadoux
authors.forename: Sébastien
authors.initials: S
authors.lastname: Blugeon
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bertho
authors.forename: Christophe
authors.initials: C
authors.lastname: Chevalier
authors.forename: Ronan
authors.initials: R
authors.lastname: Le Goffic
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Courau
authors.forename: Pascal
authors.initials: P
authors.lastname: Molimard
authors.forename: Jean Marc
authors.initials: JM
authors.lastname: Chatel
authors.forename: Philippe
authors.initials: P
authors.lastname: Langella
authors.forename: Luis G
authors.initials: LG
authors.lastname: Bermúdez-Humarán
mesh_terms: D000818:Animals; D001835:Body Weight; D002478:Cells, Cultured; D004195:Disease Models, Animal; D007155:Immunologic Factors / Q000008:administration & dosage*; D048191:Lactiplantibacillus plantarum / Q000276:immunology / Q000302:isolation & purification*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000382:microbiology; D008168:Lung / Q000821:virology; D051379:Mice; D009975:Orthomyxoviridae / Q000472:pathogenicity*; D009976:Orthomyxoviridae Infections / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control / Q000628:therapy; D019936:Probiotics / Q000008:administration & dosage*
chemical_list: D007155:Immunologic Factors