title: Septin-containing barriers control the differential inheritance of cytoplasmic elements.
abstract: Fusion of haploid cells of Saccharomyces cerevisiae generates zygotes. We observe that the zygote midzone includes a septin annulus and differentially affects redistribution of supramolecular complexes and organelles. Redistribution across the midzone of supramolecular complexes (polysomes and Sup35p-GFP [PSI+]) is unexpectedly delayed relative to soluble proteins; however, in [psi-] × [PSI+] crosses, all buds eventually receive Sup35p-GFP [PSI+]. Encounter between parental mitochondria is further delayed until septins relocate to the bud site, where they are required for repolarization of the actin cytoskeleton. This delay allows rationalization of the longstanding observation that terminal zygotic buds preferentially inherit a single mitochondrial genotype. The rate of redistribution of complexes and organelles determines whether their inheritance will be uniform.
authors.affiliation: Pathology Department and Cell Biology Program, Case Western Reserve University, 2103 Cornell Road, Cleveland, OH 44106, USA. amt10@case.edu
authors.forename: Alan Michael
authors.initials: AM
authors.lastname: Tartakoff
authors.forename: Ilya
authors.initials: I
authors.lastname: Aylyarov
authors.forename: Purnima
authors.initials: P
authors.lastname: Jaiswal
mesh_terms: D000199:Actins / Q000378:metabolism; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D016764:Cell Polarity / Q000187:drug effects; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism*; D004058:Diffusion; D049452:Green Fluorescent Proteins / Q000378:metabolism; D040582:Inheritance Patterns; D008164:Luminescent Proteins / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D063154:Mitochondrial Dynamics / Q000187:drug effects; D009154:Mutation / Q000235:genetics; D058105:Polymerization / Q000187:drug effects; D011132:Polyribosomes / Q000187:drug effects / Q000378:metabolism; D011328:Prions / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D058112:Septins / Q000378:metabolism*; D012995:Solubility; D053778:Thiazolidines / Q000494:pharmacology; D013997:Time Factors; D015053:Zygote / Q000187:drug effects / Q000378:metabolism
chemical_list: D000199:Actins; D019086:Bridged Bicyclo Compounds, Heterocyclic; D008164:Luminescent Proteins; D011328:Prions; D053778:Thiazolidines; C413663:fluorescent protein 583; D049452:Green Fluorescent Proteins; D058112:Septins; C037067:latrunculin A