title: Antioxidant activity of an exopolysaccharide isolated from Lactobacillus plantarum C88.
abstract: A neutral exopolysaccharide (EPS), designated LPC-1, was isolated from the culture of Lactobacillus plantarum C88 and purified by ion-exchange and gel-permeation chromatography. LPC-1 had an average molecular weight of 1.15 × 10(6) Da, and it was composed of galactose and glucose with a molar ratio of 1:2. The antioxidant activity of LPC-1 was evaluated with the in vitro scavenging abilities on hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. The results indicated that LPC-1 had good scavenging ability on hydroxyl radicals. Furthermore, the protective effect of LPC-1 on H(2)O(2)-induced Caco-2 cells oxidative injury was investigated. As results, LPC-1 inhibited the formation of malondialdehyde (MDA) and raised the activities of superoxide dismutase (SOD) and total antioxidant capacities (T-AOC) in a dose-dependent manner. These results demonstrate that the EPS from L. plantarum C88 has antioxidant effects that may involve scavenging of reactive oxygen species (ROS), up-regulation of enzymatic and non-enzymatic antioxidant activities, and reduction of lipid peroxidation.
authors.affiliation: Institute of Agro-food Technology, Jilin Academy of Agricultural Sciences, Changchun 130033, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Chunhong
authors.initials: C
authors.lastname: Liu
authors.forename: Da
authors.initials: D
authors.lastname: Li
authors.forename: Yujuan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xue
authors.initials: X
authors.lastname: Zhang
authors.forename: Xianpeng
authors.initials: X
authors.lastname: Zeng
authors.forename: Zhennai
authors.initials: Z
authors.lastname: Yang
authors.forename: Shengyu
authors.initials: S
authors.lastname: Li
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D018938:Caco-2 Cells; D002852:Chromatography, Ion Exchange; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007700:Kinetics; D048191:Lactiplantibacillus plantarum / Q000737:chemistry*; D008315:Malondialdehyde / Q000378:metabolism; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D012015:Reference Standards; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D011134:Polysaccharides; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D013482:Superoxide Dismutase; D001205:Ascorbic Acid