title: Cloning and characterization of the dopamine like receptor in the oyster Crassostrea angulata: expression during the ovarian cycle.
abstract: We cloned and characterized a complete cDNA encoding a dopamine receptor (DAR) named Ca-DA1R from Fujian oyster, Crassostrea angulata. The 2843 bp long cDNA sequence includes a 916-bp 5'-UTR, the 1197 bp ORF which encodes a putative protein of 399 amino acids, and a 729 bp 3'-UTR. The Ca-DA1R sequence possesses typical characteristics of a D1 receptor: two main features being a short third intracellular loop and a long inner COOH-terminal tail domain. Using a real-time PCR approach, expression profiles of Ca-DA1R were analyzed in adult tissues and during the four stages of ovarian development. Ca-DA1R was expressed ubiquitously, although transcript levels varied between tissues, with higher mRNA levels detected in the ovary, labial palps and mantle. During the four stages of ovarian development, Ca-DA1R mRNA expression level was higher in the proliferation stage than in the other three stages during the ovary cycle. In situ hybridization results reveal that the Ca-DA1R mRNA is mainly expressed in the epithelium of the gonoducts. These observations suggest that Ca-DA1R binding of DA probably plays an important role in early ovarian development and via regulating oocyte locomotion cooperates with the 5-HT receptor system during the ovarian cycle in C. angulata.
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, PR China.
authors.forename: Bingye
authors.initials: B
authors.lastname: Yang
authors.forename: Jianbin
authors.initials: J
authors.lastname: Ni
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zeng
authors.forename: Bo
authors.initials: B
authors.lastname: Shi
authors.forename: Weiwei
authors.initials: W
authors.lastname: You
authors.forename: Caihuan
authors.initials: C
authors.lastname: Ke
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D049896:Crassostrea / Q000166:cytology / Q000235:genetics* / Q000502:physiology; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D010053:Ovary / Q000166:cytology / Q000502:physiology*; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011954:Receptors, Dopamine / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013997:Time Factors; D014018:Tissue Distribution / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D011954:Receptors, Dopamine