title: Correlation of cell membrane lipid profiles with daptomycin resistance in methicillin-resistant Staphylococcus aureus.
abstract: We compared the cell membrane (CM) lipid composition among nine well-characterized daptomycin-susceptible (Dap(s))/Dap-resistant (Dap(r)) methicillin-resistant Staphylococcus aureus (MRSA) strain pairs. Compared to the 9 Dap(s) parental strains, Dap(r) strains (with or without mprF-yycFG mutations) exhibited significantly reduced phosphatidylglycerol (PG) content (P < 0.01), significantly increased total synthesis of lysyl-PG (LPG) (P < 0.01), and reduced carotenoid content (P < 0.05 for 5/9 strains). There were no significant changes in LPG flipping, cardiolipin content, or fatty acid composition among strain pairs.
authors.affiliation: Division of Infectious Diseases, Los Angeles Biomedical Research Institute at Harbor, University of California at Los Angeles, Medical Center, Torrance, California, USA. nmishra@labiomed.org
authors.forename: Nagendra N
authors.initials: NN
authors.lastname: Mishra
authors.forename: Arnold S
authors.initials: AS
authors.lastname: Bayer
mesh_terms: D019881:Aminoacyltransferases / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D002308:Cardiolipins / Q000378:metabolism; D002338:Carotenoids / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D017576:Daptomycin / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D010715:Phosphatidylglycerols / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002308:Cardiolipins; D005227:Fatty Acids; D008563:Membrane Lipids; D010715:Phosphatidylglycerols; C119939:YycF protein, Bacteria; D002338:Carotenoids; C002285:lysylphosphatidylglycerol; D019881:Aminoacyltransferases; C429146:mprF protein, Staphylococcus aureus; D008239:Lysine; D017576:Daptomycin