title: B-cell translocation gene 2: expression in the rat ovary and potential association with adenine nucleotide translocase 2 in mitochondria.
abstract: The B-cell translocation gene 2 (Btg2) is an anti-proliferative tumor suppressor gene that behaves as a transcriptional regulator. The present study investigated gonadotropin induction of Btg2 in the rat ovary and the mechanism of Btg2 action as a partner of mitochondrial protein adenine nucleotide translocase 2 (Ant2). Transient induction of Btg2 as well as Btg1 mRNA levels by LH/hCG was observed in ovarian granulosa cells. Btg2 protein levels were also stimulated by LH/hCG. LH-induced gene expression of Btg2 required ERK signal pathway. Studies of deletion mutants in HeLa cells showed that deletion of Btg2 C-terminus (Btg2/ΔC) abolished the interaction with Ant2. In fact, the expression levels of Btg2/ΔC construct were decreased in mitochondrial fraction. Btg2 was also expressed in mitochondria and interacted with Ant2 in preovulatory granulosa cells. Interestingly, a Btg2/ΔC construct inhibited an action of Btg2 wild-type on ATP and H(2)O(2) production. These findings demonstrate the gonadotropin stimulation of Btg2 in the ovary and, the physical interaction of Btg2 with Ant2 in mitochondria.
authors.affiliation: Korea Basic Science Institute, Gwangju Center at Chonnam National University, Gwangju, Republic of Korea.
authors.forename: Jae-Il
authors.initials: JI
authors.lastname: Park
authors.forename: Sun-Gyun
authors.initials: SG
authors.lastname: Kim
authors.forename: Min Woo
authors.initials: MW
authors.lastname: Baek
authors.forename: Tae Jun
authors.initials: TJ
authors.lastname: Park
authors.forename: In Kyoung
authors.initials: IK
authors.lastname: Lim
authors.forename: Young-Woo
authors.initials: YW
authors.lastname: Seo
authors.forename: Sang-Young
authors.initials: SY
authors.lastname: Chun
mesh_terms: D033742:Adenine Nucleotide Translocator 2 / Q000235:genetics / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D017874:Immediate-Early Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012756:Sheep; D025521:Tumor Suppressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D033742:Adenine Nucleotide Translocator 2; C521959:BTG2 protein, rat; C489553:Btg1 protein, rat; D006063:Chorionic Gonadotropin; D017874:Immediate-Early Proteins; D009363:Neoplasm Proteins; D025521:Tumor Suppressor Proteins; D000255:Adenosine Triphosphate; D007986:Luteinizing Hormone; D006861:Hydrogen Peroxide