title: The effect of triamcinolone acetonide, sodium hyaluronate, and chondroitin sulfate on human endothelial cells: an in vitro study.
abstract: OBJECTIVE
  To report the in vitro effect of triamcinolone acetonide (TA), sodium hyaluronate (SH), chondroitin sulfate (CS), and their combination on human endothelial cells.
  
  
  METHODS
  The antiangiogenic proprieties of 2 formulations of TA (TA-1 and TA-2), 2 formulations of glycosaminoglycan-containing viscoelastic agents (V-1 and V-2), and the association of TA-1 and V-1 were investigated. Their effect on angiogenesis was tested using human endothelial cells cocultured with human fibroblasts and myoblasts. After fixation and staining for CD31, a colorimetric output was obtained. A BCIP/NBT-buffered substrate allowed image analysis of tubule formation.
  
  
  RESULTS
  Both formulations of TA significantly reduced tubule formation as compared with controls (p<0.01). Moreover, the antiangiogenic effect of TA-1 was maintained when combined with V-1 (p<0.01).
  
  
  CONCLUSIONS
  Triamcinolone acetonide alone or in combination with hyaluronate and chondroitin sulfate is able to significantly reduce human endothelial cell proliferation in an in vitro model.
authors.affiliation: Department of Ophthalmology, University of Udine, Udine, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Veritti
authors.forename: Laura
authors.initials: L
authors.lastname: Perissin
authors.forename: Sonia
authors.initials: S
authors.lastname: Zorzet
authors.forename: Paolo
authors.initials: P
authors.lastname: Lanzetta
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000494:pharmacology*; D004359:Drug Therapy, Combination; D042783:Endothelial Cells / Q000187:drug effects*; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D014222:Triamcinolone Acetonide / Q000494:pharmacology*
chemical_list: D020533:Angiogenesis Inhibitors; D000893:Anti-Inflammatory Agents; D005938:Glucocorticoids; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; D014222:Triamcinolone Acetonide