title: A recombinant protein based on Trypanosoma cruzi P21 enhances phagocytosis.
abstract: BACKGROUND
  P21 is a secreted protein expressed in all developmental stages of Trypanosoma cruzi. The aim of this study was to determine the effect of the recombinant protein based on P21 (P21-His(6)) on inflammatory macrophages during phagocytosis.
  
  
  RESULTS
  Our results showed that P21-His(6) acts as a phagocytosis inducer by binding to CXCR4 chemokine receptor and activating actin polymerization in a way dependent onthe PI3-kinase signaling pathway.
  
  
  CONCLUSIONS
  Thus, our results shed light on the notion that native P21 is a component related to T. cruzi evasion from the immune response and that CXCR4 may be involved in phagocytosis. P21-His(6) represents an important experimental control tool to study phagocytosis signaling pathways of different intracellular parasites and particles.
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Brazil.
authors.forename: Adele A
authors.initials: AA
authors.lastname: Rodrigues
authors.forename: Tatiana M
authors.initials: TM
authors.lastname: Clemente
authors.forename: Marlus A
authors.initials: MA
authors.lastname: Dos Santos
authors.forename: Fabrício C
authors.initials: FC
authors.lastname: Machado
authors.forename: Rafael G B
authors.initials: RG
authors.lastname: Gomes
authors.forename: Heline Hellen T
authors.initials: HH
authors.lastname: Moreira
authors.forename: Mário C
authors.initials: MC
authors.lastname: Cruz
authors.forename: Paula C
authors.initials: PC
authors.lastname: Brígido
authors.forename: Paulo C F
authors.initials: PC
authors.lastname: Dos Santos
authors.forename: Flávia A
authors.initials: FA
authors.lastname: Martins
authors.forename: Diana
authors.initials: D
authors.lastname: Bahia
authors.forename: Juliana T
authors.initials: JT
authors.lastname: Maricato
authors.forename: Luiz M R
authors.initials: LM
authors.lastname: Janini
authors.forename: Eduardo H
authors.initials: EH
authors.lastname: Reboredo
authors.forename: Renato A
authors.initials: RA
authors.lastname: Mortara
authors.forename: Claudio V
authors.initials: CV
authors.lastname: da Silva
mesh_terms: D000818:Animals; D051379:Mice; D008810:Mice, Inbred C57BL; D010587:Phagocytosis; D011485:Protein Binding; D015800:Protozoan Proteins / Q000378:metabolism*; D019718:Receptors, CXCR4 / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000378:metabolism*
chemical_list: D015800:Protozoan Proteins; D019718:Receptors, CXCR4; D011994:Recombinant Proteins