title: [Establishment of a murine model of hepatic steatosis induced by chronic viral hepatitis].
abstract: OBJECTIVE
  To establish a animal model of hepatic steatosis induced by chronic viral hepatitis in C(57)BL/6 mice.
  
  
  METHODS
  C(57)BL/6 mice were randomly assigned to control group, high-fat diet group, mouse hepatitis virus strain A59 (MHV-A59) virus infection group, and high-fat diet plus virus infection group. At 13 weeks of the experiment, serum samples were collected to detect MHV antibodies and transaminase and lipid levels. The hepatic pathologies of the mice were examined with Oil red O staining of the frozen sections the and HE staining of paraffin-embedded sections.
  
  
  RESULTS
  The mice in the two virus infection groups showed strong positivity of MHV antibodies in the serum. Compared with the control group, the mice in high-fat diet group and the two virus infection groups had significantly increased AST and ALT levels with also elevated TC and LDL-C levels. The two virus infection groups both exhibited obvious pathologies in the liver characteristic of chronic viral hepatitis with increased lipid accumulation in the hepatocytes.
  
  
  CONCLUSIONS
  We have successfully established a mouse model of hepatic steatosis induced by chronic viral hepatitis, which provides the basis for further study of the disease mechanism.
authors.affiliation: School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China. gan_lu@yahoo.com.cn
authors.forename: Lu
authors.initials: L
authors.lastname: Gan
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.forename: Jinqiang
authors.initials: J
authors.lastname: Guo
authors.forename: Qian
authors.initials: Q
authors.lastname: Xie
authors.forename: Zijun
authors.initials: Z
authors.lastname: Meng
authors.forename: Weiren
authors.initials: W
authors.lastname: Wan
authors.forename: Bingde
authors.initials: B
authors.lastname: Luo
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002908:Chronic Disease; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D005234:Fatty Liver / Q000821:virology*; D006521:Hepatitis, Chronic / Q000821:virology*; D051379:Mice; D008810:Mice, Inbred C57BL; D006517:Murine hepatitis virus
chemical_list: D000914:Antibodies, Viral