title: Antimicrobial and biosensing ultrasound-responsive lysozyme-shelled microbubbles.
abstract: Air-filled lysozyme microbubbles (LSMBs) were engineered as a support for the immobilization of gold nanoparticles and an enzyme, alkaline phosphatase, in order to develop micro-antimicrobial and biosensing devices. Gold nanoparticles immobilized on LSMBs significantly improved the antimicrobial efficacy of the microbubbles against M. lysodeikticus. The surface functionalization of the microbubbles with gold nanoparticles did not affect their echogenicity when exposed to an ultrasound imaging probe. Alkaline phosphatase was conjugated on the surface of microbubbles without compromising its enzymatic activity. The functionalized microbubbles were used for the detection of paraoxon in aqueous solutions.
authors.affiliation: Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata, 00173 Roma, Italy. francesca.cavalieri@uniroma2.it
authors.forename: Francesca
authors.initials: F
authors.lastname: Cavalieri
authors.forename: Laura
authors.initials: L
authors.lastname: Micheli
authors.forename: Subramanian
authors.initials: S
authors.lastname: Kaliappan
authors.forename: Boon M
authors.initials: BM
authors.lastname: Teo
authors.forename: Meifang
authors.initials: M
authors.lastname: Zhou
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Palleschi
authors.forename: Muthupandian
authors.initials: M
authors.lastname: Ashokkumar
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D015374:Biosensing Techniques / Q000295:instrumentation*; D016503:Drug Delivery Systems / Q000295:instrumentation*; D045423:Microbubbles; D008837:Micrococcus / Q000187:drug effects; D009113:Muramidase / Q000737:chemistry* / Q000494:pharmacology; D053758:Nanoparticles / Q000737:chemistry*; D010261:Paraoxon / Q000032:analysis; D010575:Pesticides / Q000032:analysis; D014465:Ultrasonics; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; D010575:Pesticides; D014874:Water Pollutants, Chemical; D009113:Muramidase; D010261:Paraoxon