title: Influence of antioxidant complex on the adhesion of leukocytes in chronic venous insufficiency of lower limbs in rats.
abstract: OBJECTIVE
  In this study, we have evaluated the influence of antioxidant complex (AOC) (dihydroquercetin and lipoic acid) on pool of nonadhesive leukocytes and leukocytes with low and high adhesion in chronic venous insufficiency (CVI) of lower limbs in rats.
  
  
  METHODS
  Model of CVI of lower limbs was created by blood flow restriction in caudal vena cava. AOC was administered at 100 mg/kg b.w./day for 14 days p.o. The venous pressure in vena cava and adhesion of leukocytes were estimated on the 14th day of the experiment.
  
  
  RESULTS
  On the 14th day, the venous pressure in groups of animals (experimental and control) was in 2.7-2.9-fold higher. In the control group, the significant increase of leukocytes pool with high adhesion was detected. Intragastric administration of AOC reduced the number of leukocytes with high adhesion and increased the number of nonadhesive leukocytes and leukocytes with a low adhesion.
  
  
  CONCLUSIONS
  Model of CVI of lower limb is accompanied by increased venous pressure and raised adhesion activity of leukocytes. Administration of AOC for 14 days reduces the adhesive activity of leukocytes.
authors.affiliation: Institute of Pharmacology, Siberian Branch, Russian Academy of Medical Sciences, Lenin Avenue 3, 634028, Tomsk, Russian Federation, Russia.
authors.forename: Mark
authors.initials: M
authors.lastname: Plotnikov
authors.forename: Ivan
authors.initials: I
authors.lastname: Ivanov
authors.forename: Anastasia
authors.initials: A
authors.lastname: Sidehmenova
authors.forename: Oleg
authors.initials: O
authors.lastname: Aliev
authors.forename: Nonna
authors.initials: N
authors.lastname: Tykavkina
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D002448:Cell Adhesion; D007962:Leukocytes / Q000187:drug effects* / Q000502:physiology; D035002:Lower Extremity / Q000503:physiopathology; D008297:Male; D011794:Quercetin / Q000008:administration & dosage / Q000031:analogs & derivatives*; D051381:Rats; D017208:Rats, Wistar; D008063:Thioctic Acid / Q000008:administration & dosage*; D014689:Venous Insufficiency / Q000188:drug therapy* / Q000503:physiopathology; D014690:Venous Pressure
chemical_list: D000975:Antioxidants; D008063:Thioctic Acid; D011794:Quercetin; C003377:taxifolin