title: [Effects of chemokine-like factor 1 on the proliferation and apoptosis of human vascular smooth muscle cell].
abstract: OBJECTIVE
  To explore the effects of chemokine-like factor 1 (CKLF1) on the growth and apoptosis of human vascular smooth muscle cell and understand its mechanism for the induction of cell apoptosis.
  
  
  METHODS
  After the transfection of human vascular smooth muscle cell with CKLF1, CCK8 assay was used to observe the growth of cell. The cell cycle and apoptosis was assessed by flow cytometry (FCM). And the mRNA and protein expressions of Bcl-2, Bax and Caspase-3 were detected by real-time polymerase chain reaction (PCR) and Western blot respectively.
  
  
  RESULTS
  At 24, 48 and 72 h, the growth proliferation (15.8% ± 3.4%, 25.5% ± 9.8% and 37.2% ± 3.3%, P < 0.05) and inhibited apoptosis (5.5% ± 1.3%, 7.6% ± 0.7% and 11.4% ± 5.5%, P < 0.05) of vascular smooth muscle cell were detected. CKLF1 also down-regulated the level of mRNA expression of Bax (0.96 ± 0.14, 0.61 ± 0.15 and 0.37 ± 0.05), protein expression of Caspase-3 (1.94 ± 0.30, 1.09 ± 0.17 and 0.73 ± 0.09) and elevated the mRNA expression of Bcl-2 (1.59 ± 0.18, 2.05 ± 0.51 and 2.96 ± 0.27).
  
  
  CONCLUSIONS
  CKLF1 can induce proliferation and inhibit apoptosis of human vascular smooth muscle cell through the down-regulation of Bax and Caspase-3 and the up-regulation of Bcl-2 expression.
authors.affiliation: Department of Vascular Surgery, General Hospital of People's Liberation Army, Beijing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Jiang
authors.initials: J
authors.lastname: Xiong
authors.forename: Chen-yang
authors.initials: CY
authors.lastname: Shen
authors.forename: Sen-hao
authors.initials: SH
authors.lastname: Jia
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
mesh_terms: D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D018925:Chemokines / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D062792:MARVEL Domain-Containing Proteins / Q000235:genetics / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D014162:Transfection; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494826:BAX protein, human; C432676:CKLF protein, human; D018925:Chemokines; D062792:MARVEL Domain-Containing Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051028:bcl-2-Associated X Protein; C505407:CASP3 protein, human; D053148:Caspase 3