title: Detection of single nucleotide polymorphisms by PCR conformation-difference gel electrophoresis.
abstract: The most common genetic variations in the human genome, single nucleotide polymorphisms (SNPs), are ideal biomarkers and are used extensively in disease research. Here we introduce a novel method of PCR-conformation-difference gel electrophoresis (PCR-CDGE) used for detecting SNPs. The principle of PCR-CDGE relies PCR products from different homozygous DNA samples showing dissimilar migration patterns upon PAGE due to their conformational differences. PCR products from heterozygous DNA samples may exhibit two or more bands in PAGE because of the existence of DNA homoduplexes and heteroduplexes. In this study, analysis of two SNPs showed that PCR-CDGE is an accurate, simple, rapid, low-cost, and high-throughput genotyping method that could be used in most laboratories.
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Nanchang University, Nanchang 330006, China. zwf100@163.com
authors.forename: Weifeng
authors.initials: W
authors.lastname: Zhu
authors.forename: Yan
authors.initials: Y
authors.lastname: Deng
authors.forename: Kemin
authors.initials: K
authors.lastname: Jie
authors.forename: Daya
authors.initials: D
authors.lastname: Luo
authors.forename: Zhuoqi
authors.initials: Z
authors.lastname: Liu
authors.forename: Lehan
authors.initials: L
authors.lastname: Yu
authors.forename: Erming
authors.initials: E
authors.lastname: Zeng
authors.forename: Fusheng
authors.initials: F
authors.lastname: Wan
mesh_terms: D003365:Costs and Cost Analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005838:Genotype; D020180:Heteroduplex Analysis; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D013997:Time Factors