title: Presence of a novel exon 2E encoding a putative transmembrane protein in human IL-33 gene.
abstract: Interleukin-33 (IL-33) is a dual-function molecule that regulates gene expression in nuclei and, as a cytokine, conveys proinflammatory signals from outside of cells via its specific receptor ST2L. There are still a lot of questions about localization and processing of IL-33 gene products. In the course of re-evaluating human IL-33 gene, we found distinct promoter usage depending on the cell type, similar to the case in the ST2 gene. Furthermore, we found a novel exon 2E in the conventional intron 2 whose open reading frame corresponded to a transmembrane protein of 131 amino acids. Dependence of exon 2E expression on differentiation of HUVEC cells is of great interest in relation to human IL-33 function.
authors.affiliation: Department of Biochemistry, School of Medicine, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan. shintomi@jichi.ac.jp
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Tominaga
authors.forename: Morisada
authors.initials: M
authors.lastname: Hayakawa
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Tsuda
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ohta
authors.forename: Ken
authors.initials: K
authors.lastname: Yanagisawa
mesh_terms: D000595:Amino Acid Sequence; D005091:Exons / Q000235:genetics*; D057809:HEK293 Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D000067596:Interleukin-33; D007378:Interleukins / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D011401:Promoter Regions, Genetic
chemical_list: C506536:IL33 protein, human; D000067596:Interleukin-33; D007378:Interleukins; D008565:Membrane Proteins