title: The point mutation induced by the low-energy N+ ion implantation in impatiens balsamine genome.
abstract: To investigate the effect of genome mutations induced by low energy ions implantation in higher plants, genome mutation of Impatiens balsamine mutant induced by low energy N+ ion implantation were analyzed by the RAPD, ISSR and genome sequence. Six out of the 121 ISSR primers and 6 out of the 135 RAPD primers showed that polymorphism ratios between mutants and wild type were 4.96% and 2.89%, respectively. Sequence analysis revealed that base deletions, insertions, and substitutions were observed in the mutant genome comparable to wild type. N+ induced point mutations were mostly base substitution (77.4%), no duplication, long fragments insertions and deletions was found. In all point mutation, adenine (A) was most sensitive to the N+ ion implantation in impatiens. The transition was mainly A --> guanine (G) (15.90%) and thymine (T) --> cytosine (C) (12.55%). Transversion happened in A <--> T (16.74%), which much higher than C <--> G(5.02%), G <--> T(6.69%), A <--> C (7.11%) bases. These findings indicate that low energy ions being a useful mutagen were mostly cause the point mutation in impatiens.
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang 453007, China. gaowujun@yahoo.cn
authors.forename: W J
authors.initials: WJ
authors.lastname: Gao
authors.forename: J X
authors.initials: JX
authors.lastname: Su
authors.forename: L
authors.initials: L
authors.lastname: Xie
authors.forename: C L
authors.initials: CL
authors.lastname: Deng
authors.forename: T
authors.initials: T
authors.lastname: Zhang
authors.forename: L D
authors.initials: LD
authors.lastname: Lu
mesh_terms: D018745:Genome, Plant / Q000528:radiation effects*; D029069:Impatiens / Q000033:anatomy & histology / Q000235:genetics / Q000528:radiation effects; D007477:Ions / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity; D009584:Nitrogen / Q000633:toxicity*; D017354:Point Mutation / Q000528:radiation effects*; D011827:Radiation; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA
chemical_list: D007477:Ions; D009153:Mutagens; D009584:Nitrogen