title: Proteomic analysis showed down-regulation of nucleophosmin in progressive tumor cells compared to regressive tumor cells.
abstract: Important strategies against cancer are based on the understanding of the mechanisms of tumor progression. To elucidate alterations regarding tumor progression, we have performed proteomic differential display analysis for the expression of intracellular proteins in the regressive murine fibrosarcoma cell clone QR-32 and the progressive malignant tumor cell clone QRsP-11, derived from QR-32, by means of combination of two-dimensional gel electrophoresis (2-DE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS), and we have previously reported on relevant results. However, besides the protein spots which we already reported, we identified three more particular spots of interest. In the present study, two-dimensional western blot analysis demonstrated a significantly lower expression of three isoforms of nucleophosmin in progressive, compared to regressive cell clones. These results suggest that the down-regulation of the identified nucleophosmin proteins in QRsP-11 cells compared to QR-32 cells is possibly related to tumor malignant progression.
authors.affiliation: Department of Biochemistry and Functional Proteomics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-kogushi, Ube, Yamaguchi 755-8505, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Takenawa
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kuramitsu
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Wang
authors.forename: Futoshi
authors.initials: F
authors.lastname: Okada
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Tokuda
authors.forename: Takao
authors.initials: T
authors.lastname: Kitagawa
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Ueyama
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005354:Fibrosarcoma / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D000090243:Nucleophosmin; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D053719:Tandem Mass Spectrometry
chemical_list: C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D020033:Protein Isoforms; D000090243:Nucleophosmin