title: Hematopoietic recovery following chemotherapy is improved by BADGE-induced inhibition of adipogenesis.
abstract: This study was designed to investigate the role of increased adipocytes in the bone marrow (BM) niche induced by high-dose chemotherapy in hematopoietic recovery. Arabinosylcytosine (Ara-C) was administered to adult C57BL/6J mice to induce adipogenesis in the BM. We investigated the effects of adipogenesis on hematopoietic recovery following chemotherapy, using the peroxisome proliferator-activated receptor gamma inhibitor, bisphenol A diglycidyl ether (BADGE). Adipocyte hyperplasia could be induced by Ara-C treatment in BM and inhibited by BADGE. The accelerated recovery of leukocyte counts, increased colony forming units, and a higher proportion of Ki67(+)CD45(+) BM cells and Ki67(+)Lin(-)Sca1(+)c-kit(+) hematopoietic stem cells were observed in the long bone marrow of adipocyte-inhibited mice, as well as an increase in the number of CD45(+) BM cells in the tail fatty marrow compared to controls. Adipocytes participated in creating a distinctive niche for hematopoietic cells. In addition, lower expression of stromal cell-derived factor-1α and hypoxia-inducible factor-1 alpha were detected in the BADGE-treated group. These results indicate that hematopoietic recovery is improved following chemotherapy in adipogenesis-inhibited mice. In addition, adipocytes may create an individual niche that affects the proliferation and migration of hematopoietic cells in vitro and in vivo.
authors.affiliation: Peking University People's Hospital and Peking University Institute of Hematology, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation for the Treatment of Hematological Diseases, 11 Xizhimen South Street, Xicheng District, Beijing, China.
authors.forename: Rong-Jia
authors.initials: RJ
authors.lastname: Zhu
authors.forename: Meng-Qing
authors.initials: MQ
authors.lastname: Wu
authors.forename: Zi-Jian
authors.initials: ZJ
authors.lastname: Li
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhang
authors.forename: Kai-Yan
authors.initials: KY
authors.lastname: Liu
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D000970:Antineoplastic Agents / Q000009:adverse effects; D001559:Benzhydryl Compounds / Q000494:pharmacology*; D001853:Bone Marrow / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003561:Cytarabine / Q000009:adverse effects; D004852:Epoxy Compounds / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression; D006410:Hematopoiesis / Q000187:drug effects* / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006965:Hyperplasia; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism; D007958:Leukocyte Count; D051379:Mice; D047495:PPAR gamma / Q000037:antagonists & inhibitors*; D000098826:Bisphenol A Compounds
chemical_list: D000970:Antineoplastic Agents; D001559:Benzhydryl Compounds; D003561:Cytarabine; D004852:Epoxy Compounds; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D047495:PPAR gamma; C019273:bisphenol A diglycidyl ether; D000098826:Bisphenol A Compounds