title: Aquaporin OsPIP1;1 promotes rice salt resistance and seed germination.
abstract: OsPIP1;1 is one of the most abundant aquaporins in rice leaves and roots and is highly responsible to environmental stresses. However, its biochemical and physiological functions are still largely unknown. The oocyte assay data showed OsPIP1;1 had lower water channel activity in contrast to OsPIP2;1. EGFP and immunoelectron microscopy studies revealed OsPIP1;1 was predominantly localized in not only plasma membrane but also in some ER-like intracellular compartments in the cells. OsPIP1;1 exhibited low water channel activity in Xenopus oocytes but coexpression of OsPIP2;1 significantly enhanced its water permeability. Stop-flow assay indicated that 10His-OsPIP1;1-reconstituted proteoliposomes had significantly higher water permeability than the control liposomes. Overexpression of OsPIP1;1 greatly altered many physiological features of transgenic plants in a dosage-dependent manner. Moderate expression of OsPIP1;1 increased rice seed yield, salt resistance, root hydraulic conductivity, and seed germination rate. This work suggests OsPIP1;1 functions as an active water channel and plays important physiological roles.
authors.affiliation: Graduate School of Bioresource Sciences, Akita Prefectural University, Shimoshinjo, Akita 010-0195, Japan.
authors.forename: Chengwei
authors.initials: C
authors.lastname: Liu
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Fukumoto
authors.forename: Tadashi
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Patrizia
authors.initials: P
authors.lastname: Gena
authors.forename: Daniele
authors.initials: D
authors.lastname: Frascaria
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Kaneko
authors.forename: Maki
authors.initials: M
authors.lastname: Katsuhara
authors.forename: Shihua
authors.initials: S
authors.lastname: Zhong
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Sun
authors.forename: Yanming
authors.initials: Y
authors.lastname: Zhu
authors.forename: Ikuko
authors.initials: I
authors.lastname: Iwasaki
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Ding
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Calamita
authors.forename: Yoshichika
authors.initials: Y
authors.lastname: Kitagawa
mesh_terms: D020346:Aquaporins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D018525:Germination / Q000187:drug effects*; D012275:Oryza / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*; D014867:Water / Q000378:metabolism
chemical_list: D020346:Aquaporins; D010940:Plant Proteins; D014867:Water; D012965:Sodium Chloride