title: Luman recruiting factor regulates endoplasmic reticulum stress in mouse ovarian granulosa cell apoptosis.
abstract: Follicular atresia is primarily induced by granulosa cell apoptosis; however, the molecular mechanisms that control apoptotic cell death in granulosa cells remain poorly understood. The present studies were undertaken to investigate the role of a novel endoplasmic reticulum stress-regulated gene Luman recruiting factor (LRF) in granulosa cell apoptosis during mouse follicular atresia. Based on immunohistochemistry and confocal laser scanning microscope analysis, LRF protein was localized in the cytoplasm of apoptotic granulosa cells, similar to localization of the LRF, Luman, CCAAT/enhancer-binding protein homologous protein and caspase-12 proteins were localized in apoptotic granulosa cells. However, glucose-regulated protein 78 protein was only present in healthy cells of the mural granulosa cell layers. A spontaneous onset of apoptotic cell death of granulosa cells was induced by thapsigargin or tunicamycin treatment in vitro, which was closely related to the increase of LRF, Luman, CCAAT/enhancer-binding protein homologous protein, and caspase-12 mRNA. Taken together, LRF might be involved in inducing apoptosis of granulosa cells through the endoplasmic reticulum stress pathway and might have a key role in mouse follicular selection.
authors.affiliation: Key Laboratory of Animal Biotechnology, Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, PR China.
authors.forename: Yanzhou
authors.initials: Y
authors.lastname: Yang
authors.forename: Pengfei
authors.initials: P
authors.lastname: Lin
authors.forename: Fenglei
authors.initials: F
authors.lastname: Chen
authors.forename: Aihua
authors.initials: A
authors.lastname: Wang
authors.forename: Xiangli
authors.initials: X
authors.lastname: Lan
authors.forename: Yujie
authors.initials: Y
authors.lastname: Song
authors.forename: Yaping
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053454:Caspase 12 / Q000235:genetics; D017362:Cyclic AMP Response Element-Binding Protein / Q000032:analysis / Q000502:physiology*; D003593:Cytoplasm / Q000737:chemistry; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress / Q000502:physiology*; D005260:Female; D005496:Follicular Atresia / Q000502:physiology; D006107:Granulosa Cells / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D006360:Heat-Shock Proteins / Q000235:genetics; D007150:Immunohistochemistry; D051379:Mice; D018613:Microscopy, Confocal; D012333:RNA, Messenger / Q000032:analysis; D019284:Thapsigargin / Q000494:pharmacology; D014415:Tunicamycin / Q000494:pharmacology
chemical_list: C116472:Creb3 protein, mouse; D017362:Cyclic AMP Response Element-Binding Protein; D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D014415:Tunicamycin; D019284:Thapsigargin; D053454:Caspase 12