title: A method of chemiluminescence coupled with ultrafiltration for investigating the interaction between ibuprofen and human serum albumin.
abstract: In acidic media, ibuprofen substantially enhanced the weak chemiluminescence (CL) produced by sodium sulfite and potassium permanganate. The increased signals were linearly correlated with ibuprofen concentrations ranging from 1.2 × 10(-3) to 4.8 μM, with a detection limit of 4.8 × 10(-4) μM. Two ultrafiltration (UF) membranes were used to construct a unit for trapping 0.15 and 0.75 μM human serum albumin (HSA) and coupled online with the CL system. At low HSA concentrations, the numbers of bound molecules per binding site were calculated to be 0.9 for Sudlow site I and 6.2 for Sudlow site II. The association constants on these binding sites were 5.9 × 10(5) and 3.4 × 10(4) M(-1), respectively. Our CL-UF protocol presents a rapid and sensitive method for studies on drug-protein interaction.
authors.affiliation: College of Chemistry and Chemical Engineering, Xi'an Shiyou University, Xi'an, 710065, China.
authors.forename: Xunyu
authors.initials: X
authors.lastname: Xiong
authors.forename: Qunzheng
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yefei
authors.initials: Y
authors.lastname: Nan
authors.forename: Xuefan
authors.initials: X
authors.lastname: Gu
mesh_terms: D006801:Humans; D007052:Ibuprofen / Q000737:chemistry*; D049449:Luminescence; D008163:Luminescent Measurements; D012709:Serum Albumin / Q000737:chemistry*; D014462:Ultrafiltration
chemical_list: D012709:Serum Albumin; D007052:Ibuprofen