title: Endovascular treatment of unruptured intracranial aneurysms and circulating endothelial cells.
abstract: OBJECTIVE
  To evaluate the potential implication of circulating endothelial cells (CECs) in complications following endovascular treatment (EVT) of unruptured intracranial aneurysms. CECs characterized as CD146(+)/CD105(+)/CD45(-)/DAPI(+) were considered to originate from an altered endothelial cell layer of the vessel wall.
  
  
  METHODS
  In 15 patients, CECs were characterized and enumerated by the CellTracks(®) System in blood samples from: (1) femoral artery (FA), (2) internal carotid artery (ICA) before (ICA1) and after procedure (ICA2), and (3) a peripheral vein before (PV1) and after EVT (PV2). Ischemic brain events were assessed using diffusion weighted imaging (DWI-MRI) before and 24h after EVT.
  
  
  RESULTS
  In ICA1, the median number of single CECs and clusters of 2-5 CECs were higher than in FA, ICA2, PV1 and PV2 samples (P<0.001). Clusters >5 cells, sometimes >50μm, were mainly observed in ICA1 and never in PV1, PV2 or PV samples from ten healthy subjects. This distribution of CECs suggested femoral and ICA injury by the devices used, leading to endothelium shearing and desquamation of CECs. All patients discharged on day two (NIHSS score=0), however silent ischemic brain lesions were observed in 9/15 (60%).
  
  
  CONCLUSIONS
  EVT detaches single and clusters of CECs from wall arteries that may be implicated in silent ischemic brain lesions genesis. Enumeration of CECs associated with DWI-MRI might represent an interesting strategy for monitoring and optimizing endovascular devices, and further limit EVT-related complications.
authors.affiliation: Department of Neuroradiology, University Hospital Center, Guy de Chauliac Hospital, 80 Avenue Augustin Fliche, 34295 Montpellier Cedex, France. jfvendrell@yahoo.fr
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Vendrell
authors.forename: Renaud
authors.initials: R
authors.lastname: Cezar
authors.forename: Nils
authors.initials: N
authors.lastname: Kuster
authors.forename: Kyriakos
authors.initials: K
authors.lastname: Lobotesis
authors.forename: Vincent
authors.initials: V
authors.lastname: Costalat
authors.forename: Paolo
authors.initials: P
authors.lastname: Machi
authors.forename: Alain
authors.initials: A
authors.lastname: Bonafe
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Vendrell
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D038524:Diffusion Magnetic Resonance Imaging / Q000379:methods*; D042783:Endothelial Cells / Q000276:immunology*; D004730:Endothelium, Vascular / Q000276:immunology*; D057510:Endovascular Procedures / Q000009:adverse effects*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D002532:Intracranial Aneurysm / Q000276:immunology* / Q000628:therapy*; D008297:Male; D008875:Middle Aged; D012307:Risk Factors; D018709:Statistics, Nonparametric