title: Characterization of herpes simplex virus type 1 L-particle assembly and egress in hippocampal neurones by electron cryo-tomography.
abstract: Visualizing virus-host interactions in situ inside infected cells by electron cryo-tomography provides unperturbed snapshots of the infection process. Here we focus on the assembly and egress pathway of herpesviruses. Cells infected with herpes simplex virus 1 produce and release not only infective virions but also non-infectious light particles (L-particles). L-particles are devoid of viral capsids and genomes. In this study, we analysed L-particle assembly and egress pathways in cultured dissociated hippocampus neurones by electron cryo-tomography. Virion and L-particle formation occurred in close proximity, suggesting shared assembly and exit pathways. Clathrin-like coats were occasionally associated with L-particle and virion assembly sites. Further, we compared the three-dimensional ultrastructure of intracellular and extracellular L-particles and quantified their diameters and the abundance of inclusion bodies contained.
authors.affiliation: Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Martinsried, D-82152, Germany.
authors.forename: Iosune
authors.initials: I
authors.lastname: Ibiricu
authors.forename: Ulrike E
authors.initials: UE
authors.lastname: Maurer
authors.forename: Kay
authors.initials: K
authors.lastname: Grünewald
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D002522:Chlorocebus aethiops; D020285:Cryoelectron Microscopy; D055032:Electron Microscope Tomography; D018259:Herpesvirus 1, Human / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D006624:Hippocampus / Q000166:cytology / Q000821:virology*; D054884:Host-Pathogen Interactions; D006801:Humans; D002479:Inclusion Bodies / Q000378:metabolism / Q000648:ultrastructure; D018716:Molecular Mimicry; D009474:Neurons / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure / Q000821:virology*; D010316:Particle Size; D051381:Rats; D014709:Vero Cells; D014764:Viral Proteins / Q000378:metabolism* / Q000648:ultrastructure; D014771:Virion / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D019065:Virus Assembly; D057074:Virus Release; D014779:Virus Replication
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D014764:Viral Proteins