title: Reevaluation of a lectin antibody ELISA kit for measuring fucosylated haptoglobin in various conditions.
abstract: BACKGROUND
  Fucosylated haptoglobin (Fuc-Hpt) is a novel cancer biomarker in a variety of pathological conditions. We previously found that the level of Fuc-Hpt is increased in the sera of patients with pancreatic cancer, and established a lectin antibody ELISA using Aleuria aurantia lectin, which specifically binds to fucosylated residues on oligosaccharides.
  
  
  METHODS
  To apply this assay system to the clinical detection of several diseases, several assay conditions such as serum dilutions and inhibitory factors were investigated. The Fuc-Hpt kit was available for 25-625 fold serum dilution.
  
  
  RESULTS
  While the values of Fuc-Hpt assay using sera and plasma were different, they showed positive correlation. The addition of bilirubin and formagine did not influence on Fuc-Hpt assay, but hemoglobin inhibited this assay in a dose-dependent manner.
  
  
  CONCLUSIONS
  We reevaluated this lectin antibody ELISA kit for measuring fucosylated haptoglobin in various conditions in this study.
authors.affiliation: Department of Molecular Biochemistry and Clinical Investigation, Osaka University Graduate School of Medicine 1-7, Yamada-oka, Suita 565-0871, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kamada
authors.forename: Noriaki
authors.initials: N
authors.lastname: Kinoshita
authors.forename: Yoko
authors.initials: Y
authors.lastname: Tsuchiya
authors.forename: Kyoko
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Hironobu
authors.initials: H
authors.lastname: Fujii
authors.forename: Naoko
authors.initials: N
authors.lastname: Terao
authors.forename: Kyoko
authors.initials: K
authors.lastname: Kamihagi
authors.forename: Nobuto
authors.initials: N
authors.lastname: Koyama
authors.forename: Shin
authors.initials: S
authors.lastname: Yamada
authors.forename: Yataro
authors.initials: Y
authors.lastname: Daigo
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Taniguchi
authors.forename: Eiji
authors.initials: E
authors.lastname: Miyoshi
mesh_terms: D000906:Antibodies / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005643:Fucose / Q000378:metabolism*; D006242:Haptoglobins / Q000378:metabolism*; D006801:Humans; D037102:Lectins / Q000276:immunology* / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000097:blood / Q000175:diagnosis; D015203:Reproducibility of Results
chemical_list: D000906:Antibodies; D006242:Haptoglobins; D037102:Lectins; C064549:lectin, Aleuria aurantia; D005643:Fucose