title: Graph based study of allergen cross-reactivity of plant lipid transfer proteins (LTPs) using microarray in a multicenter study.
abstract: The study of cross-reactivity in allergy is key to both understanding. the allergic response of many patients and providing them with a rational treatment In the present study, protein microarrays and a co-sensitization graph approach were used in conjunction with an allergen microarray immunoassay. This enabled us to include a wide number of proteins and a large number of patients, and to study sensitization profiles among members of the LTP family. Fourteen LTPs from the most frequent plant food-induced allergies in the geographical area studied were printed into a microarray specifically designed for this research. 212 patients with fruit allergy and 117 food-tolerant pollen allergic subjects were recruited from seven regions of Spain with different pollen profiles, and their sera were tested with allergen microarray. This approach has proven itself to be a good tool to study cross-reactivity between members of LTP family, and could become a useful strategy to analyze other families of allergens.
authors.affiliation: Centro de Biotecnología y Genómica de Plantas UPM-INIA, Campus de Montegancedo, Pozuelo de Alarcón, Madrid, Spain.
authors.forename: Arantxa
authors.initials: A
authors.lastname: Palacín
authors.forename: Cristina
authors.initials: C
authors.lastname: Gómez-Casado
authors.forename: Luis A
authors.initials: LA
authors.lastname: Rivas
authors.forename: Jacobo
authors.initials: J
authors.lastname: Aguirre
authors.forename: Leticia
authors.initials: L
authors.lastname: Tordesillas
authors.forename: Joan
authors.initials: J
authors.lastname: Bartra
authors.forename: Carlos
authors.initials: C
authors.lastname: Blanco
authors.forename: Teresa
authors.initials: T
authors.lastname: Carrillo
authors.forename: Javier
authors.initials: J
authors.lastname: Cuesta-Herranz
authors.forename: Consolación
authors.initials: C
authors.lastname: de Frutos
authors.forename: Genoveva García
authors.initials: GG
authors.lastname: Alvarez-Eire
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Fernández
authors.forename: Pedro
authors.initials: P
authors.lastname: Gamboa
authors.forename: Rosa
authors.initials: R
authors.lastname: Muñoz
authors.forename: Rosa
authors.initials: R
authors.lastname: Sánchez-Monge
authors.forename: Sofía
authors.initials: S
authors.lastname: Sirvent
authors.forename: María J
authors.initials: MJ
authors.lastname: Torres
authors.forename: Susana
authors.initials: S
authors.lastname: Varela-Losada
authors.forename: Rosalía
authors.initials: R
authors.lastname: Rodríguez
authors.forename: Victor
authors.initials: V
authors.lastname: Parro
authors.forename: Miguel
authors.initials: M
authors.lastname: Blanca
authors.forename: Gabriel
authors.initials: G
authors.lastname: Salcedo
authors.forename: Araceli
authors.initials: A
authors.lastname: Díaz-Perales
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000276:immunology*; D052179:Antigens, Plant / Q000276:immunology*; D002352:Carrier Proteins / Q000276:immunology*; D003429:Cross Reactions / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000737:chemistry; D005502:Food; D005512:Food Hypersensitivity / Q000276:immunology; D005843:Geography; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008055:Lipids / Q000737:chemistry; D015233:Models, Statistical; D010940:Plant Proteins / Q000276:immunology*; D011058:Pollen; D040081:Protein Array Analysis / Q000379:methods*; D011994:Recombinant Proteins / Q000737:chemistry; D013030:Spain; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D002352:Carrier Proteins; D000939:Epitopes; D008055:Lipids; D010940:Plant Proteins; D011994:Recombinant Proteins; C511920:lipid transfer proteins, plant