title: Coupled production of single cell oil as biodiesel feedstock, xylitol and xylanase from sugarcane bagasse in a biorefinery concept using fungi from the tropical mangrove wetlands.
abstract: This work evaluates sugarcane bagasse (SCB) conversion, in a biorefinery approach, to coproduce biodiesel and high value products using two novel mangrove fungi. On acid pre-treatment, sugarcane bagasse hydrolysate (SCBH) resulted in a xylitol yield of 0.51 g/g xylose consumed in 72 h by Williopsis saturnus. After SCB pretreatment, sugarcane bagasse residue (SCBR) was utilized using Aspergillus terreus for production of xylanase (12.74 U/ml) and cell biomass (9.8 g/L) which was extracted for single cell oil (SCO; 0.19 g/g) and transesterified to biodiesel. The FAME profile exhibited long chain SFAs and PUFAs with predicted biodiesel properties lying within the range specified by international standards. This biorefining approach of SCB utilization for co-production of xylitol, xylanase and SCO gains importance in terms of sustainability and eco-friendliness.
authors.affiliation: Department of Biotechnology, University of Pune, Pune 411 007, India.
authors.forename: Srijay
authors.initials: S
authors.lastname: Kamat
authors.forename: Mahesh
authors.initials: M
authors.lastname: Khot
authors.forename: Smita
authors.initials: S
authors.lastname: Zinjarde
authors.forename: Ameeta
authors.initials: A
authors.lastname: RaviKumar
authors.forename: Wasudeo Namdeo
authors.initials: WN
authors.lastname: Gade
mesh_terms: D001230:Aspergillus / Q000378:metabolism; D032407:Avicennia / Q000382:microbiology; D061249:Batch Cell Culture Techniques; D056804:Biofuels / Q000382:microbiology*; D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry*; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis*; D004952:Esters / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005658:Fungi / Q000201:enzymology / Q000378:metabolism*; D006868:Hydrolysis; D009821:Oils / Q000378:metabolism*; D031786:Saccharum / Q000737:chemistry; D013997:Time Factors; D014329:Tropical Climate; D053833:Wetlands; D014993:Xylitol / Q000096:biosynthesis*
chemical_list: D056804:Biofuels; D004952:Esters; D005227:Fatty Acids; D009821:Oils; D002482:Cellulose; C027433:bagasse; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases; D014993:Xylitol