title: Genetic organization and conjugal plasmid DNA transfer of pHP69, a plasmid from a Korean isolate of Helicobacter pylori.
abstract: We isolated pHP69, a 9,153 bp plasmid from Helicobacter                 pylori with a 33.98% (G+C) content. We identified 11 open reading frames (ORFs), including replication initiation protein A (repA), fic (cAMP-induced filamentation protein), mccC, mccB, mobA, mobD, mobB, and mobC, as well as four 22 bp tandem repeat sequences. The nucleic acid and predicted amino acid sequences of these ORFs exhibited significant homology to those of other H. pylori plasmids. pHP69 repA encodes a replication initiation protein and its amino acid sequence is similar to those of replicase proteins from theta-type plasmids. pHP69 contains two types of repeat sequences (R1 and R2), a MOBHEN family mobilization region comprising mobC, mobA, mobB, and mobD, and genes encoding microcin B and C. Among the 36 H. pylori strains containing plasmids, mobA or mccBC are present in 12 or 6, respectively and 3 contain both genes. To examine intrinsic capability of H. pylori for conjugative plasmid transfer, a shuttle vector pBHP69KH containing pHP69 and replication origin of pBR322 was constructed. It was shown that this vector could stably replicate and be mobilized among clinical H. pylori strains and demonstrated to gene transfer by natural plasmid.
authors.affiliation: Laboratory of Biochemistry and Genetics, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Jung-Soo
authors.initials: JS
authors.lastname: Joo
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Song
authors.forename: Seung-Chul
authors.initials: SC
authors.lastname: Baik
authors.forename: Woo-Kon
authors.initials: WK
authors.lastname: Lee
authors.forename: Myung-Je
authors.initials: MJ
authors.lastname: Cho
authors.forename: Kon-Ho
authors.initials: KH
authors.lastname: Lee
authors.forename: YoungAh
authors.initials: Y
authors.lastname: Cho
authors.forename: Hee-Shang
authors.initials: HS
authors.lastname: Youn
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Seo
authors.forename: Kwang-Ho
authors.initials: KH
authors.lastname: Rhee
authors.forename: Hyung-Lyun
authors.initials: HL
authors.lastname: Kang
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D017124:Conserved Sequence; D000242:Cyclic AMP / Q000378:metabolism; D023061:Gene Order; D005822:Genetic Vectors / Q000235:genetics; D016480:Helicobacter pylori / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D000242:Cyclic AMP