title: miR-24-3p and miR-27a-3p promote cell proliferation in glioma cells             via cooperative regulation of MXI1.
abstract: MicroRNAs (miRNAs) are small, non‑coding RNAs which regulate gene expression             at the post-transcriptional level. Abnormal expression of miRNAs occurs frequently             in tumors. Although the two miRNAs miR‑24‑3p and miR‑27a‑3p come from two duplicated             gene clusters of miR‑23a~27a~24‑2 and miR‑23b~27b~24‑1 which are found to be deregulated             in a variety of cancers, the role of cooperation of the two clusters and the function             of the two miRNAs in tumors have not been completely characterized. Here, we show             that overexpression of miR‑24‑3p and miR‑27a‑3p could promote cell proliferation             using the MTT assay. By integrated bioinformatic analysis and experimental confirmation,             we identified MXI1, which has been found to act as a tumor suppressor gene by             affecting c‑Myc, as a direct target of miR‑24‑3p and miR‑27a‑3p. While targeting             the MXI1 3' untranslated region by miR‑24‑3p or miR‑27a‑3p, luciferase activity             was attenuated. The two miRNAs promote glioma cell proliferation via targeting             MXI1 and the experiment was confirmed by the rescue experiments. Furthermore,             our results show that two clusters of miR-23a~27a~24-2 and miR‑23b~27b~24‑1 regulate             MXI1 synergistically. These findings reveal, for the first time, the novel functions             of cooperation of miR‑24‑3p and miR‑27a‑3p from two clusters in promoting cell             proliferation through MXI1. Additionally, we observed that miR‑27a‑3p is upregulated             in glioma tissues.
authors.affiliation: Key Laboratory of Gene Engineering of the Ministry of Education,                     State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen                     University, Guangzhou, P.R. China.
authors.forename: Weiyi
authors.initials: W
authors.lastname: Xu
authors.forename: Mingfa
authors.initials: M
authors.lastname: Liu
authors.forename: Xueling
authors.initials: X
authors.lastname: Peng
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.forename: Jianwen
authors.initials: J
authors.lastname: Zhou
authors.forename: Ke
authors.initials: K
authors.lastname: Xu
authors.forename: Haixiong
authors.initials: H
authors.lastname: Xu
authors.forename: Songshan
authors.initials: S
authors.lastname: Jiang
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D035683:MicroRNAs; D025521:Tumor Suppressor Proteins; C543845:MIRN24 microRNA, human; C531388:MIRN27 microRNA, human; C079440:MXI1 protein, human