title: [Analysis of serum neutralizing antibody response in patients with primary dengue virus type 1 infection].
abstract: OBJECTIVE
  To investigate the characteristics and dynamic changes of serum neutralizing antibody response in patients with primary infection of dengue virus type 1 (DENV-1).
  
  
  METHODS
  Serum samples were obtained from the same patients with primary infection of DENV-1 within 2 weeks after symptom onset in 2006 and in 2010. A group-specific DENV NS1 capture ELISA-based micro-neutralizing test (ELISA-MNT) capable of detecting neutralizing antibodies against all the 4 serotypes of DENV was used to test the neutralizing antibody titers against DENV in the serum samples. The neutralizing antibody titers against a standard strain and 2 clinically isolated strains of DENV-1 were detected in serum samples collected in 2010.
  
  
  RESULTS
  Cross-reactive neutralizing antibody response against all the 4 serotypes of DENV was found in both of the serum samples collected in 2006 and 2010, but the samples collected in 2006 showed stronger cross-reactive neutralizing antibody responses. The neutralizing antibody against DENV-2, rather than the anticipated DENV-1 antibody, had the highest titer in the samples collected in 2006, whereas the antibody against homologous DENV-1 had the highest titer in the samples obtained in 2010. The neutralizing antibody titers against the homologous DENV-1 was significantly higher in samples collected in 2010 (U=86.500, P=0.000), which also demonstrated significantly different neutralizing antibody titers against the 3 different strains of DENV-1 (Χ(2)=12.123, P=0.002).
  
  
  CONCLUSIONS
  The production of cross-reactive neutralizing antibodies between the 4 serotypes of DENV is a characteristic of DENV infection, particularly during early infection, but only the homologous neutralizing antibody increases obviously over time. The titers of the neutralizing antibodies against different strains, even of the same serotype, may differ distinctly.
authors.affiliation: Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. zjyyhdm@163.com
authors.forename: Dongmei
authors.initials: D
authors.lastname: Hu
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Dahu
authors.initials: D
authors.lastname: Wang
authors.forename: Biao
authors.initials: B
authors.lastname: DI
authors.forename: Liwen
authors.initials: L
authors.lastname: Qiu
authors.forename: Yadi
authors.initials: Y
authors.lastname: Wang
authors.forename: Xixia
authors.initials: X
authors.lastname: Ding
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Che
mesh_terms: D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D003429:Cross Reactions; D003715:Dengue / Q000097:blood* / Q000276:immunology*; D003716:Dengue Virus / Q000145:classification / Q000276:immunology*; D006801:Humans; D009500:Neutralization Tests
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral