title: Pterostilbene induces cell cycle arrest and apoptosis in MOLT4 human leukemia cells.
abstract: Pterostilbene, a polyphenolic compound present in grapes and other fruits, has been demonstrated to inhibit growth and induce apoptosis and autophagy in some cancer cell types. We found that pterostilbene at the IC(90) concentration of 44 µM inhibited proliferation and induced apoptosis in MOLT4 human leukemia cells. Treatment with pterostilbene resulted in a transient accumulation of cells in the G(0)/G(1)-cell cycle phase followed by the S-phase arrest. Pterostilbene-induced apoptotic death of MOLT4 cells was mediated by caspase-3 activation and was accompanied by the disruption of mitochondrial membrane potential, phosphatidylserine externalization and internucleosomal DNA fragmentation. Our results suggest that pterostilbene could serve as a potential additional chemotherapeutic agent for the treatment of leukemia.
authors.affiliation: Department of Histology, Medical University of Gdansk, Gdansk, Poland. ksiedlecka@gumed.edu.pl
authors.forename: Kamila
authors.initials: K
authors.lastname: Siedlecka-Kroplewska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Jozwik
authors.forename: Lucyna
authors.initials: L
authors.lastname: Kaszubowska
authors.forename: Anna
authors.initials: A
authors.lastname: Kowalczyk
authors.forename: Wojciech
authors.initials: W
authors.lastname: Boguslawski
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D007938:Leukemia / Q000188:drug therapy* / Q000473:pathology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013267:Stilbenes / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*
chemical_list: D017382:Reactive Oxygen Species; D013267:Stilbenes; C107773:pterostilbene