title: Cryopreserved chondrocytes in porous biomaterials with surface elastin and poly-L-lysine for cartilage regeneration.
abstract: The ability of cryopreserved chondrocytes to revitalize and propagate is a key biotechnology in cartilage regeneration. This study shows the formation of neocartilage from cryopreserved chondrocytes in scaffolds grafted with elastin and poly-L-lysine. Cryopreserved chondrocytes in elastin- and poly-L-lysine-grafted constructs were cultured in a dynamic bioreactor and assessed by biochemical assay and staining. Elastin demonstrated a better efficacy for recruiting cryopreserved chondrocytes onto the pore surface of constructs than poly-L-lysine. However, surface elastin and poly-L-lysine did not significantly enhance the biocompatibility to cryopreserved chondrocytes. Chondrocytes multiplied from cryopreserved chondrocytes in elastin-grafted constructs is faster than that in poly-L-lysine-grafted constructs. In addition, elastin could stimulate cryopreserved chondrocytes to synthesize more glycosaminoglycans and collagen than poly-L-lysine. Porous biomaterials with surface elastin and poly-L-lysine can maintain active chondrocytic proliferation and extracellular matrix secretion from chondrocytes with appropriate cryopreservation.
authors.affiliation: Buddhist Tzu-Chi Dalin General Hospital, Chia-Yi 62247, Taiwan, ROC.
authors.forename: Shaw-Ruey
authors.initials: SR
authors.lastname: Lyu
authors.forename: Yung-Chih
authors.initials: YC
authors.lastname: Kuo
authors.forename: Hao-Fu
authors.initials: HF
authors.lastname: Ku
authors.forename: Wen-Hsin
authors.initials: WH
authors.lastname: Hsieh
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002356:Cartilage / Q000187:drug effects / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D003094:Collagen / Q000096:biosynthesis; D015925:Cryopreservation; D004549:Elastin / Q000494:pharmacology*; D006025:Glycosaminoglycans / Q000378:metabolism; D011107:Polylysine / Q000494:pharmacology*; D016062:Porosity; D012038:Regeneration / Q000187:drug effects*; D013194:Staining and Labeling; D013499:Surface Properties; D034421:Sus scrofa; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D006025:Glycosaminoglycans; D011107:Polylysine; D003094:Collagen; D004549:Elastin