title: In vitro degradation and drug release of a biodegradable tissue adhesive based on functionalized 1,2-ethylene glycol bis(dilactic acid) and chitosan.
abstract: Biodegradability and adhesive-associated local drug release are important aspects of research in tissue adhesive development. Therefore, this study focuses on investigating the in vitro degradation and drug release of a tissue adhesive consisting of hexamethylene diisocyanate functionalized 1,2-ethylene glycol bis(dilactic acid) and chitosan chloride. To prevent infections, ciprofloxacin hydrochloride (CPX·HCl) was incorporated into the adhesive. The influence of CPX·HCl on the adhesive reaction and adhesive strength was analyzed by FTIR-ATR-spectroscopy and tensile tests. The CPX·HCl release was investigated by HPLC. The degradation-induced changes at 37 °C were evaluated by gravimetric/morphological analyzes and micro-computer tomography. The antibiotic potential of the CPX·HCl loaded adhesive was determined by agar diffusion tests. The degradation tests revealed a mass loss of about 78 % after 52 weeks. The adhesive reaction velocity and tensile strength were not influenced by CPX·HCl. Using a 2 mg/g CPX·HCl loaded adhesive an inhibition of all tested bacteria was observed.
authors.affiliation: Institute for Biomedical Engineering, University of Rostock, Rostock, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Lurtz
authors.forename: Karsten
authors.initials: K
authors.lastname: Voss
authors.forename: Veronika
authors.initials: V
authors.lastname: Hahn
authors.forename: Frieder
authors.initials: F
authors.lastname: Schauer
authors.forename: Juergen
authors.initials: J
authors.lastname: Wegmann
authors.forename: Erich K
authors.initials: EK
authors.lastname: Odermatt
authors.forename: Klaus-Peter
authors.initials: KP
authors.lastname: Schmitz
authors.forename: Katrin
authors.initials: K
authors.lastname: Sternberg
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D019855:Ethylene Glycol / Q000737:chemistry*; D066298:In Vitro Techniques; D019344:Lactic Acid / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D017550:Spectroscopy, Fourier Transform Infrared; D013718:Tensile Strength; D014014:Tissue Adhesives
chemical_list: D014014:Tissue Adhesives; D019344:Lactic Acid; D048271:Chitosan; D019855:Ethylene Glycol