title: [Establishment of a nude mouse model of orthotopic engineered gastric tumor and its in vivo fluorescence imaging].
abstract: OBJECTIVE
  To establish a nude mouse model of orthotopic engineered gastric tumor for in vivo fluorescence imaging studies.
  
  
  METHODS
  An engineered gastric tumor was constructed in vitro using collagen as the scaffold and the human gastric cancer cell line BGC823-EGFP cells expressing enhanced green fluorescence protein (EGFP) as the seed cells. The engineered tumor was then implanted into the stomach of nude mice, and the tumor growth was observed with in vivo fluorescence imaging. The nude mice were sacrificed 6 weeks after the transplantation to assess the tumor growth and metastasis, and the tumor histology was evaluated.
  
  
  RESULTS
  The tumor cells in the engineered tumor model grew well in three-dimensional culture. The success rate of orthotopic gastric tumor implantation was 100% (10/10) in nude mice with metastasis in the abdominal organs. The isolated tumor mass, weighing 1.719∓0.349 g, showed a histological characteristic of poorly differentiated adenocarcinoma. In vivo fluorescence imaging detected EGFP-expressing tumors in the abdominal cavity of the nude mice, but not accurately.
  
  
  CONCLUSIONS
  The nude mouse model bearing orthotopic engineered gastric tumor provides a simple animal model for the study of gastric cancer, but a stronger fluorescence than green fluorescence is more desirable for more effective observation in in vivo fluorescence imaging.
authors.affiliation: Institute of General Surgery, General Hospital of PLA, Beijing 100853, China. E-mail: spm301@163.com.
authors.forename: Peiming
authors.initials: P
authors.lastname: Sun
authors.forename: Runsen
authors.initials: R
authors.lastname: Jin
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Du
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Xu
authors.forename: Huiwei
authors.initials: H
authors.lastname: Sun
authors.forename: Rong
authors.initials: R
authors.lastname: Li
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000032:analysis; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D061848:Optical Imaging; D013274:Stomach Neoplasms; D023822:Tissue Engineering
chemical_list: D049452:Green Fluorescent Proteins