title: Purification and structural characterisation of lipid transfer protein from red wine and grapes.
abstract: Lipid transfer proteins (LTP) play a major role in plant defence and are of particular interest due to their known ability to cause allergic reactions. These proteins are expressed in grapes and also remain detectable after vinification, especially in red wine. However, it remains unknown whether the protein undergoes any changes during the vinification process. Here, we present a purification method for LTPs from Dornfelder grapes and wine. By liquid-chromatography-mass spectroscopy (LC-MS/MS) we identified LTPs from two different species (Vitis vinifera and Vitis aestivalis). Additionally, the purified LTPs were characterised using spectrometric methods, confirming their high purity and structural stability during vinification. We conclude that LTPs are resistant to the alcohol content (13.5 vol%), acidic milieu of wine and other ingredients present during the vinification process, indicating that the allergenic potential of grape LTP is not diminished by the vinification process.
authors.affiliation: Institute for Molecular Biophysics, Johannes Gutenberg-University Mainz, Jakob Welder-Weg 26, 55128 Mainz, Germany.
authors.forename: Nadine
authors.initials: N
authors.lastname: Jaeckels
authors.forename: Stefan
authors.initials: S
authors.lastname: Tenzer
authors.forename: Susanne
authors.initials: S
authors.lastname: Rosfa
authors.forename: Hansjoerg
authors.initials: H
authors.lastname: Schild
authors.forename: Heinz
authors.initials: H
authors.lastname: Decker
authors.forename: Petra
authors.initials: P
authors.lastname: Wigand
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D011487:Protein Conformation; D053719:Tandem Mass Spectrometry; D027843:Vitis / Q000737:chemistry*; D014920:Wine / Q000032:analysis*
chemical_list: D002352:Carrier Proteins; D010940:Plant Proteins; C030363:lipid transfer protein