title: Functional analysis of Hsp70 superfamily proteins of rice (Oryza sativa).
abstract: Heat stress results in misfolding and aggregation of cellular proteins. Heat shock proteins (Hsp) enable the cells to maintain proper folding of proteins, both in unstressed as well as stressed conditions. Hsp70 genes encode for a group of highly conserved chaperone proteins across the living systems encompassing bacteria, plants, and animals. In the cellular chaperone network, Hsp70 family proteins interconnect other chaperones and play a dominant role in various cell processes. To assess the functionality of rice Hsp70 genes, rice genome database was analyzed. Rice genome contains 32 Hsp70 genes. Rice Hsp70 superfamily genes are represented by 24 Hsp70 family and 8 Hsp110 family members. Promoter and transcript expression analysis divulges that Hsp70 superfamily genes plays important role in heat stress. Ssc1 (mitochondrial Hsp70 protein in yeast) deleted yeast show compromised growth at 37 °C. Three mitochondrial rice Hsp70 sequences (i.e., mtHsp70-1, mtHsp70-2, and mtHsp70-3) complemented the Ssc1 mutation of yeast to differential extents. The information presented in this study provides detailed understanding of the Hsp70 protein family of rice, the crop species that is the major food for the world population.
authors.affiliation: Department of Plant Molecular Biology, University of Delhi South Campus, N Delhi, India.
authors.forename: Neelam K
authors.initials: NK
authors.lastname: Sarkar
authors.forename: Preeti
authors.initials: P
authors.lastname: Kundnani
authors.forename: Anil
authors.initials: A
authors.lastname: Grover
mesh_terms: D000595:Amino Acid Sequence; D030541:Databases, Genetic; D018745:Genome, Plant; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D050436:Regulatory Elements, Transcriptional; D013696:Temperature; D059467:Transcriptome
chemical_list: D018840:HSP70 Heat-Shock Proteins