title: Evidence for an alternative genomic structure, mRNA and protein sequence of human ABCA13.
abstract: ABC transporters form one of the major families of transport proteins. In humans, the ABC family comprises seven subfamilies named A to G, of which the A subfamily contains twelve members. Among these are several well-characterized transporters, including ABCA1, which is involved in cellular cholesterol transport and HDL formation, and ABCA4, which is a transporter for vitamin A derivatives in photoreceptor cells. The function of another subfamily member termed ABCA13 is unknown. The human ABCA13 gene has been reported to span 450kb of genomic DNA at chromosomal locus 7p12.3 and to encode a 5058 amino acid protein that includes two unusually large exons close to the N-terminus. We now show that the gene as well as the corresponding mRNA and protein may be considerably shorter than previously thought. We used PCR and RACE to identify a genomic sequence spanning about 350kb and encoding a protein of 2323 amino acids. This corresponds to the C-terminal half of the previously reported ABCA13 protein but lacks the residues reportedly encoded by the two very big N-terminal exons. Using immunoprecipitation and Western blot analyses we identified a protein of about 260kDa in size likely representing the shorter protein proposed here. Computer analyses showed that our proposed sequence contains all the structural elements of an ABCA protein and agrees well with the mouse ABCA13 protein sequence. Additionally, we identified a putative promoter region containing well-conserved TATA and CAAT boxes just upstream of our transcription start site. Overall, our data provide good evidence for an alternative human ABCA13 transcript and protein.
authors.affiliation: Institute of Nutrition, Friedrich Schiller University Jena, Germany.
authors.forename: Marten B
authors.initials: MB
authors.lastname: Maeß
authors.forename: Katrin
authors.initials: K
authors.lastname: Stolle
authors.forename: Paul
authors.initials: P
authors.lastname: Cullen
authors.forename: Stefan
authors.initials: S
authors.lastname: Lorkowski
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000378:metabolism; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D002478:Cells, Cultured; D002874:Chromosome Mapping; D017124:Conserved Sequence; D005091:Exons; D015870:Gene Expression; D015894:Genome, Human; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D006801:Humans; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D024363:Transcription Initiation Site
chemical_list: C473800:ABCA13 protein, human; D018528:ATP-Binding Cassette Transporters; D012333:RNA, Messenger