title: Effects of controlled release of brain-derived neurotrophic factor from collagen gel on rat neural stem cells.
abstract: This study aimed to determine the effects of the controlled release of brain-derived neurotrophic factor (BDNF) from collagen gel on rat neural stem cells (NSCs). With two groups of daily addition of BDNF and collagen gel without BDNF as controls, BDNF was tested using ELISA at different time points. In the BDNF-collagen gel group, BDNF was steadily released from gels for 10 days. The cell viability test and the bromodeoxyuridine incorporation assay showed that the BDNF-collagen gel supported the survival and proliferation of NSCs. Compared with controls, the length of processes was markedly longer and the differentiation percentage from NSCs into neurons was much higher in the BDNF-collagen gel group (P<0.05). These findings suggest that BDNF-collagen gel can significantly reduce the amount of BDNF required for the culture of NSCs and increase the differentiation percentage from NSCs into neurons.
authors.affiliation: Department of Orthopaedics, The Fourth Affiliated Hospital of Anhui Medical University, Hefei, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Huang
authors.forename: Zongsheng
authors.initials: Z
authors.lastname: Yin
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.forename: Jiqing
authors.initials: J
authors.lastname: Hao
mesh_terms: D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism / Q000494:pharmacology*; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology; D003692:Delayed-Action Preparations; D004622:Embryo, Mammalian; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D007700:Kinetics; D019579:Neocortex / Q000166:cytology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D058953:Neural Stem Cells / Q000187:drug effects*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D003692:Delayed-Action Preparations; D009419:Nerve Tissue Proteins; D003094:Collagen; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D001973:Bromodeoxyuridine