title: Comparative analysis of presence of Cytomegalovirus (CMV) and Epsteinbarr virus -1 (EBV-1) in cases of chronic periodontitis and aggressive periodontitis with controls.
abstract: OBJECTIVE
  The aim of the present study was to evaluate the presence of Cytomegalovirus (CMV) and Epsteinbarr virus -1 (EBV-1)viruses in sub gingival plaque of chronic periodontitis (groupA), aggressive periodontitis patients (group B), periodontally healthy controls (group C) and to compare the clinical parameters between virus negative and positive sites in each of these groups.
  
  
  METHODS
  Sixty subjects were included in the study and equally divided into the 3 groups (group A - 20, group B - 20, group C - 20). Sub gingival plaque samples were obtained from the 3 deepest periodontal pocket sites in case of subjects suffering from periodontitis, and from one random bleeding site per quadrant in healthy groups. Clinical parameters like plaque index (PI), gingival index (GI), pocket depth (PD) and clinical loss of attachment (CAL) were recorded. Viral Deoxyribonucleic acid (DNA) was extracted using Proteinase-K DNA Extraction method, and the presence of CMV and EBV-1 was detected by polymerase chain reaction and 2% agarose gel.
  
  
  RESULTS
  Results of our study showed a 45% prevalence of CMV and EBV-1 in Aggressive periodontitis cases. Prevalence of CMV in chronic periodontitis and healthy subjects was 20% and 10%, respectively; while for EBV-1 it was 25% and 0%, respectively. In terms of comparison of the clinical parameters with virus presence, both CMV and EBV-1 positive sites showed a significantly higher mean pocket depth compared to virus negative sites.
  
  
  CONCLUSIONS
  Our studyshowed that the prevalence of EBV1 was higher in chronic and aggressive periodontitis subjects compared to controls and the prevalence of CMV was higher in aggressive periodontitis patients. The virus positive sites showed higher pocket depth compared to virus negative sites.
authors.affiliation: Department of Periodontics, Saveetha Dental College and Hospitals, Chennai, India.
authors.forename: Rupali
authors.initials: R
authors.lastname: Sharma
authors.forename: Ogoti
authors.initials: O
authors.lastname: Padmalatha
authors.forename: Gurumoorthy
authors.initials: G
authors.lastname: Kaarthikeyan
authors.forename: N D
authors.initials: ND
authors.lastname: Jayakumar
authors.forename: Sheeja
authors.initials: S
authors.lastname: Varghese
authors.forename: Khalefathullah
authors.initials: K
authors.lastname: Sherif
mesh_terms: D000328:Adult; D010520:Aggressive Periodontitis / Q000821:virology*; D055113:Chronic Periodontitis / Q000821:virology*; D003587:Cytomegalovirus / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis; D004279:DNA, Viral / Q000032:analysis; D003773:Dental Plaque / Q000821:virology; D003774:Dental Plaque Index; D004587:Electrophoresis, Agar Gel; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis; D005884:Gingival Hemorrhage / Q000145:classification / Q000821:virology; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000145:classification / Q000821:virology; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000145:classification / Q000821:virology; D010519:Periodontium / Q000821:virology; D016133:Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D004279:DNA, Viral