title: Myoblast differentiation on graphene oxide.
abstract: Graphene-based nanomaterials have received much attention in biomedical applications for drug/gene delivery, cancer therapy, imaging, and tissue engineering. Despite the capacity of 2D carbon materials as a nontoxic and implantable platform, their effect on myogenic differentiation has been rarely studied. We investigated the myotube formation on graphene-based nanomaterials, particularly graphene oxide (GO) and reduced graphene oxide (rGO). GO sheets were immobilized on amine-modified glass to prepare GO-modified glass, which was further reduced by hydrazine treatment for the synthesis of rGO-modified substrate. We studied the behavior, including adhesion, proliferation, and differentiation, of mouse myoblast C2C12 on unmodified, GO-, and rGO-modified glass substrates. According to our analyses of myogenic protein expression, multinucleate myotube formation, and expression of differentiation-specific genes (MyoD, myogenin, Troponin T, and MHC), myogenic differentiation was remarkably enhanced on GO, which resulted from serum protein adsorption and nanotopographical cues. Our results demonstrate the ability of GO to stimulate myogenic differentiation, showing a potential for skeletal tissue engineering applications.
authors.affiliation: KAIST Institute for the BioCentury, Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors.forename: Sook Hee
authors.initials: SH
authors.lastname: Ku
authors.forename: Chan Beum
authors.initials: CB
authors.lastname: Park
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006108:Graphite / Q000494:pharmacology*; D051379:Mice; D018625:Microscopy, Atomic Force; D024510:Muscle Development / Q000187:drug effects / Q000235:genetics; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D032446:Myoblasts / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D018008:Myogenin / Q000235:genetics / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D056951:Photoelectron Spectroscopy
chemical_list: D018008:Myogenin; D006108:Graphite; D009584:Nitrogen