title: Centrifugal partition extraction, a new method for direct metabolites recovery from culture broth: case study of torularhodin recovery from Rhodotorula rubra.
abstract: Centrifugal partition extraction (CPE), close to centrifugal partition chromatography, put in contact in a continuous way two immiscible liquid phases. This work presents early experiments on CPE use for solid-liquid-liquid extraction. It was applied to the direct treatment of culture broth for metabolites recovery. Torularhodin is one of the carotenoid pigments produced by the yeast Rhodotorula sp., with a terminal carboxylic group considered nowadays as a powerful antioxidant to be included in food and drugs formulations. Torularhodin was extracted from Rhodotorula rubra ICCF 209 cells by CPE. The recovery of torularhodin reaches 74 μg/g of biomass i.e. 294 μg/L of culture medium. The efficiency of the extraction step increased with the operating flow rate. The extraction yield could reach 91% with a contact time lower than 2 min. A 300 mL apparatus allowed a feed at 90 mL/min. The technique is proposed for extraction or sample preparation before analysis.
authors.affiliation: University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science 1-7, Polizu Str., 011061 Bucharest, Romania.
authors.forename: Camelia
authors.initials: C
authors.lastname: Ungureanu
authors.forename: Luc
authors.initials: L
authors.lastname: Marchal
authors.forename: Ana Aurelia
authors.initials: AA
authors.lastname: Chirvase
authors.forename: Alain
authors.initials: A
authors.lastname: Foucault
mesh_terms: D018533:Biomass; D002338:Carotenoids / Q000096:biosynthesis* / Q000302:isolation & purification*; D002498:Centrifugation / Q000379:methods; D005591:Chemical Fractionation / Q000379:methods*; D002845:Chromatography / Q000379:methods; D003470:Culture Media / Q000737:chemistry*; D005240:Feasibility Studies; D012248:Rhodotorula / Q000737:chemistry*
chemical_list: D003470:Culture Media; D002338:Carotenoids; C078923:torularhodin