title: In vitro investigation on the biodegradability and biocompatibility of genipin cross-linked porcine acellular dermal matrix with intrinsic fluorescence.
abstract: As a biocompatible and bioactive natural tissue engineering scaffold, porcine acellular dermal matrix (PADM) has limitations for the application in tissue regeneration due to its low strength and rapid biodegradation. Here, purified PADM was modified by a nontoxic cross-linker (genipin) to enhance its mechanical properties and improve its resistance to enzymatic degradation. In vitro testing results demonstrated that the stiffness of the genipin cross-linked PADM was improved and biodegradation rate was decreased. Results of cell proliferation assays showed that the cross-linking reaction by genipin did not undermine the cytocompatibility of PADM. Furthermore, genipin cross-linking imparted an observable fluorescence allowing visualization of the scaffold's three-dimensional (3D) porous structure and cell distribution by confocal laser scanning microscopy (CLSM). Immunostaining of the cell nuclei and cytoskeleton indicated that MC3T3-E1 preosteoblasts were tightly adhered to and uniformly distributed onto the cross-linked PADM scaffold. Results of this study suggest that the 3D porous genipin cross-linked PADM with intrinsic fluorescence may have broader applications for tissue engineering scaffolds where higher mechanical stiffness is needed.
authors.affiliation: Bio-Micro-Nano Functional Materials Center, State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, China.
authors.forename: Jichuan
authors.initials: J
authors.lastname: Qiu
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
authors.forename: Guancong
authors.initials: G
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Zheng
authors.forename: Na
authors.initials: N
authors.lastname: Ren
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.forename: Huaidong
authors.initials: H
authors.lastname: Jiang
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Wang
mesh_terms: D062088:Acellular Dermis; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001696:Biomechanical Phenomena; D049109:Cell Proliferation; D003432:Cross-Linking Reagents / Q000737:chemistry; D005453:Fluorescence; D039823:Iridoids / Q000737:chemistry*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology; D016062:Porosity; D013552:Swine; D023822:Tissue Engineering / Q000295:instrumentation*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D039823:Iridoids; C007834:genipin