title: Biochemical membrane lipidomics during Drosophila development.
abstract: Lipids play critical roles in energy homeostasis, membrane structure, and signaling. Using liquid chromatography and mass spectrometry, we provide a comprehensive semiquantification of lipids during the life cycle of Drosophila melanogaster (230 glycerophospholipids, 210 sphingolipids, 6 sterols and sterol esters, and 60 glycerolipids) and obtain biological insights through this biochemical resource. First, we find a high and constant triacylglycerol-to-membrane lipid ratio during pupal stage, which is nonobvious in the absence of nutrient uptake and tissue remodeling. Second, sphingolipids undergo specific changes in headgroup (glycosylation) and tail configurations (unsaturation and hydroxylation on sphingoid base and fatty acyls, respectively), which correlate with gene expression of known (GlcT/CG6437; FA2H/ CG30502) and putative (Cyt-b5-r/CG13279) enzymes. Third, we identify a gender bias in phosphoethanolamine-ceramides as a lead for future investigation into sexual maturation. Finally, we partially characterize ghiberti, required for male meiotic cytokinesis, as a homolog of mammalian serine palmitoyltransferase.
authors.affiliation: Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, 4002 Basel, Switzerland. xueli.guan@unibas.ch
authors.forename: Xue Li
authors.initials: XL
authors.lastname: Guan
authors.forename: Gianluca
authors.initials: G
authors.lastname: Cestra
authors.forename: Guanghou
authors.initials: G
authors.lastname: Shui
authors.forename: Antje
authors.initials: A
authors.lastname: Kuhrs
authors.forename: Ralf B
authors.initials: RB
authors.lastname: Schittenhelm
authors.forename: Ernst
authors.initials: E
authors.lastname: Hafen
authors.forename: F Gisou
authors.initials: FG
authors.lastname: van der Goot
authors.forename: Carmen C
authors.initials: CC
authors.lastname: Robinett
authors.forename: Maurizio
authors.initials: M
authors.lastname: Gatti
authors.forename: Marcos
authors.initials: M
authors.lastname: Gonzalez-Gaitan
authors.forename: Markus R
authors.initials: MR
authors.lastname: Wenk
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D048749:Cytokinesis / Q000502:physiology; D004331:Drosophila melanogaster / Q000254:growth & development* / Q000378:metabolism; D005260:Female; D008297:Male; D013058:Mass Spectrometry; D008540:Meiosis / Q000502:physiology; D008563:Membrane Lipids / Q000032:analysis*; D051102:Serine C-Palmitoyltransferase / Q000378:metabolism*; D012737:Sex Factors; D012741:Sexual Maturation; D013109:Sphingomyelins / Q000378:metabolism
chemical_list: D008563:Membrane Lipids; D013109:Sphingomyelins; C060281:ceramide phosphoethanolamine; D051102:Serine C-Palmitoyltransferase