title: Safety and protective efficacy of live attenuated Salmonella Gallinarum mutants in Rhode Island Red chickens.
abstract: Salmonella enterica serovar Gallinarum is the causative agent of fowl typhoid, an important systemic disease of poultry with economic consequences in developing nations. A live attenuated orally applied S. Gallinarum vaccine could provide a low cost method for controlling this disease. We constructed S. Gallinarum strains in which the expression of the crp, rfc and rfaH genes, important for virulence of Salmonella Typhimurium in mice, were under the control of an arabinose-regulated promoter. We evaluated the virulence of these strains compared to wild-type S. Gallinarum and to mutants carrying deletions in these genes. We found that rfc mutants were fully virulent, indicating that, unlike the S. Typhimurium mouse model, the rfc gene is dispensable in S. Gallinarum for virulence in birds. In the case of rfaH, the deletion mutant was attenuated and protective, while the strain with arabinose-regulated rfaH expression retained full virulence. The strain exhibiting arabinose-regulated crp expression was attenuated. Its virulence was not affected by the inclusion of 0.2% arabinose in the drinking water. Birds immunized with this strain were protected against a lethal S. Gallinarum challenge and against colonization with the human pathogen Salmonella Enteritidis. This work shows that an arabinose-regulated crp strain provides a basis for further development of a fowl typhoid vaccine.
authors.affiliation: The Biodesign Institute, School of Life Sciences, Arizona State University, Tempe, AZ 85287, United States.
authors.forename: Arindam
authors.initials: A
authors.lastname: Mitra
authors.forename: Amanda
authors.initials: A
authors.lastname: Loh
authors.forename: Amanda
authors.initials: A
authors.lastname: Gonzales
authors.forename: Paweł
authors.initials: P
authors.lastname: Laniewski
authors.forename: Crystal
authors.initials: C
authors.lastname: Willingham
authors.forename: Roy
authors.initials: R
authors.lastname: Curtiss Iii
authors.forename: Kenneth L
authors.initials: KL
authors.lastname: Roland
mesh_terms: D000818:Animals; D002645:Chickens; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D011201:Poultry Diseases / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control*; D012475:Salmonella / Q000276:immunology* / Q000472:pathogenicity; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control*; D022562:Salmonella Vaccines / Q000008:administration & dosage / Q000009:adverse effects* / Q000276:immunology*; D016019:Survival Analysis; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology; D014774:Virulence; D037521:Virulence Factors / Q000096:biosynthesis / Q000235:genetics
chemical_list: D022562:Salmonella Vaccines; D014613:Vaccines, Attenuated; D037521:Virulence Factors