title: Ionic liquids as solvents for in situ dispersive liquid-liquid microextraction of DNA.
abstract: Six ionic liquids (ILs) were applied for the first time as solvents in the extraction and preconcentration of deoxyribonucleic acid (DNA) using an in situ dispersive liquid-liquid microextraction (DLLME) approach. The effect of different IL substituents and functional group on the extraction efficiency of DNA was investigated. The highest extraction efficiencies of DNA were obtained using 1-(1,2-dihydroxypropyl)-3-hexadecylimidazolium bromide (C(16)POHIM-Br) and N,N-didecyl-N-methyl-d-glucaminium bromide [(C(10))(2)NMDG-Br]. Extraction efficiencies higher than 97% were obtained using small amounts of IL (0.50mg) for each extraction. The extraction of DNA from a sample matrix containing metal ions and protein revealed that the metal ions did not interfere with the extraction of DNA and that the co-extraction of protein can be mitigated by performing the extraction under moderately acidic conditions. Data from (31)P NMR spectroscopy suggest that a combination of electrostatic and π-π interactions dominate IL-DNA complexation and that the extraction is concentration dependant.
authors.affiliation: Department of Chemistry, The University of Toledo, 2801 W. Bancroft Street, MS 602, Toledo, OH 43606, USA.
authors.forename: Tianhao
authors.initials: T
authors.lastname: Li
authors.forename: Manishkumar D
authors.initials: MD
authors.lastname: Joshi
authors.forename: Donald R
authors.initials: DR
authors.lastname: Ronning
authors.forename: Jared L
authors.initials: JL
authors.lastname: Anderson
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D052578:Ionic Liquids / Q000737:chemistry*; D059627:Liquid Phase Microextraction / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D012997:Solvents / Q000737:chemistry*; D055672:Static Electricity; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D052578:Ionic Liquids; D012997:Solvents; D014867:Water; D004247:DNA