title: Detection of hantavirus in bats from remaining rain forest in São Paulo, Brazil.
abstract: BACKGROUND
  The significant biodiversity found in Brazil is a potential for the emergence of new zoonoses. Study in some places of the world suggest of the presence to hantavirus in tissues of bats. Researches of hantavirus in wildlife, out rodents, are very scarce in Brazil. Therefore we decided to investigate in tissues of different species of wild animals captured in the same region where rodents were detected positive for this virus. The present work analyzed ninety-one animals (64 rodents, 19 opossums, and 8 bats) from a region of the Atlantic forest in Biritiba Mirin City, São Paulo State, Brazil. Lungs and kidneys were used for RNA extraction.
  
  
  RESULTS
  The samples were screened for evidence of hantavirus infection by SYBR-Green-based real-time RT-PCR. Sixteen samples positive were encountered among the wild rodents, bats, and opossums. The detection of hantavirus in the lungs and kidneys of three marsupial species (Micoureus paraguayanus, Monodelphis ihering, and Didelphis aurita) as well in two species of bats (Diphylla ecaudata and Anoura caudifer) is of significance because these new hosts could represent an important virus reservoirs.
  
  
  CONCLUSIONS
  The analysis of nucleotide sequences of the partial S segment revealed that these genes were more related to the Araraquara virus strains. This work reinforces the importance of studying hantavirus in different animal species and performing a continued surveillance before this virus spreads in new hosts and generated serious problems in public health.
authors.affiliation: BSL3+ Laboratory, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, SP CEP: 05508-900, Brazil. jansentequila@usp.br
authors.forename: Jansen
authors.initials: J
authors.lastname: de Araujo
authors.forename: Luciano Matsumiya
authors.initials: LM
authors.lastname: Thomazelli
authors.forename: Dyana Alves
authors.initials: DA
authors.lastname: Henriques
authors.forename: Daniele
authors.initials: D
authors.lastname: Lautenschalager
authors.forename: Tatiana
authors.initials: T
authors.lastname: Ometto
authors.forename: Lilia Mara
authors.initials: LM
authors.lastname: Dutra
authors.forename: Caroline Cotrin
authors.initials: CC
authors.lastname: Aires
authors.forename: Sandra
authors.initials: S
authors.lastname: Favorito
authors.forename: Edison Luiz
authors.initials: EL
authors.lastname: Durigon
mesh_terms: D000818:Animals; D001483:Base Sequence; D001938:Brazil / Q000453:epidemiology; D002685:Chiroptera / Q000821:virology*; D004197:Disease Reservoirs / Q000821:virology; D062665:Epidemiological Monitoring; D006476:Orthohantavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018778:Hantavirus Infections / Q000453:epidemiology* / Q000635:transmission / Q000662:veterinary* / Q000821:virology; D007668:Kidney / Q000821:virology; D008168:Lung / Q000821:virology; D008969:Molecular Sequence Data; D009893:Opossums / Q000821:virology*; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012377:Rodentia / Q000821:virology*; D014197:Trees; D014329:Tropical Climate