title: Proteomic analysis of developing rye grain with contrasting resistance to preharvest sprouting.
abstract: Significant differences in the two-dimensional electrophoresis patterns of proteins from developing rye grain were found to be associated with resistance and susceptibility to preharvest sprouting (PHS). Mass spectrometry of individual spots showing different abundance in PHS-resistant and PHS-susceptible lines identified proteins involved in: reaction to biotic and abiotic stresses, including oxidative stress, energy metabolism and regulation of gene expression. Highly differentiated abundance of proteins found in developing grain suggest that the diversification of processes leading to developing PHS resistance or PHS susceptibility starts from an early stage of grain development. A part of the identified proteins in rye grain were also reported to be associated with PHS in wheat and rice, which suggests that some mechanisms affecting precocious germination might be common for different cereal species.
authors.affiliation: West Pomeranian University of Technology, Słowackiego 17, 71-434, Szczecin, Poland. pmasojc@zut.edu.pl
authors.forename: Piotr
authors.initials: P
authors.lastname: Masojć
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Kosmala
authors.forename: Dawid
authors.initials: D
authors.lastname: Perlikowski
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D004734:Energy Metabolism; D018506:Gene Expression Regulation, Plant; D018525:Germination / Q000235:genetics*; D012275:Oryza / Q000235:genetics; D018384:Oxidative Stress; D010940:Plant Proteins / Q000032:analysis*; D018520:Plant Shoots / Q000235:genetics; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D012434:Secale / Q000235:genetics* / Q000254:growth & development*; D014908:Triticum / Q000235:genetics / Q000254:growth & development
chemical_list: D010940:Plant Proteins; D020543:Proteome