title: Expression of antigen tf and galectin-3 in fibroadenoma.
abstract: BACKGROUND
  Fibroadenomas are benign human breast tumors, characterized by proliferation of epithelial and stromal components of the terminal ductal unit. They may grow, regress or remain unchanged, as the hormonal environment of the patient changes. Expression of antigen TF in mucin or mucin-type glycoproteins and of galectin-3 seems to contribute to proliferation and transformations events; their expression has been reported in ductal breast cancer and in aggressive tumors.
  
  
  RESULTS
  Lectin histochemistry, immunohistochemistry, and immunofluorescence were used to examine the expression and distribution of antigen TF and galectin-3. We used lectins from Arachis hypogaea, Artocarpus integrifolia, and Amaranthus lecuocarpus to evaluate TF expression and a monoclonal antibody to evaluate galectin-3 expression. We used paraffin-embedded blocks from 10 breast tissues diagnosed with fibroadenoma and as control 10 healthy tissue samples. Histochemical and immunofluorescence analysis showed positive expression of galectin-3 in fibroadenoma tissue, mainly in stroma, weak interaction in ducts was observed; whereas, in healthy tissue samples the staining was also weak in ducts. Lectins from A. leucocarpus and A. integrifolia specificaly recognized ducts in healthy breast samples, whereas the lectin from A. hypogaea recognized ducts and stroma. In fibroadenoma tissue, the lectins from A. integrifolia, A. Hypogaea, and A. leucocarpus recognized mainly ducts.
  
  
  CONCLUSIONS
  Our results suggest that expression of antigen TF and galectin-3 seems to participate in fibroadenoma development.
authors.affiliation: Centro de Investigaciones en Ciencias Medicas y Biológicas Facultad de Medicina, Universidad Autónoma Benito Juárez de Oaxaca, 68020, Oaxaca, Mexico.
authors.forename: Itandehui Belem
authors.initials: IB
authors.lastname: Gallegos
authors.forename: Eduardo
authors.initials: E
authors.lastname: Pérez-Campos
authors.forename: Margarito
authors.initials: M
authors.lastname: Martinez
authors.forename: Miguel Ángel
authors.initials: MÁ
authors.lastname: Mayoral
authors.forename: Laura
authors.initials: L
authors.lastname: Pérez
authors.forename: Sergio
authors.initials: S
authors.lastname: Aguilar
authors.forename: Edgar
authors.initials: E
authors.lastname: Zenteno
authors.forename: Maria del Socorro
authors.initials: Mdel S
authors.lastname: Pina
authors.forename: Pedro
authors.initials: P
authors.lastname: Hernández
mesh_terms: D000951:Antigens, Neoplasm / Q000378:metabolism*; D001943:Breast Neoplasms / Q000276:immunology* / Q000378:metabolism; D005260:Female; D018226:Fibroadenoma / Q000276:immunology* / Q000378:metabolism; D037502:Galectin 3 / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry
chemical_list: D000951:Antigens, Neoplasm; D037502:Galectin 3