title: Simultaneous determination for oxicam non-steroidal anti-inflammatory drugs in human serum by liquid chromatography-tandem mass spectrometry.
abstract: A high-performance liquid chromatography-tandem mass spectrometry (LC/MS/MS) technique was developed for the simultaneous determination of five non-steroidal anti-inflammatory oxicam drugs (ampiroxicam, tenoxicam, piroxicam, meloxicam and lornoxicam) in human plasma. These five oxicam drugs and isoxicam (internal standard) were extracted from human plasma with an Oasis(®) MAX cartridge column and analysed on a Unison UK-C18 column (2.0 mm × 100 mm, 3 μm) with an acetonitrile:10mM formic ammonium buffer (pH 3.0) (50:50) mobile phase at 0.20 ml/min at 37°C. The analytes were detected using a tandem mass spectrometer, equipped with an electrospray ion source (ESI). The instrument was used in multiple-reaction-monitoring (MRM) mode. The extraction yields from a 200 μl human plasma sample (containing 10 ng of each drugs) with the Oasis(®) MAX cartridge column were 93.3-102.5%. The detection limits were 0.01-6.5 ng/ml (S/N=3). Our developed method is very useful for the simultaneous determination of five oxicam (non-steroidal anti-inflammatory) drugs in human plasma by LC/MS/MS.
authors.affiliation: Department of Pathophysiology, Yokohama College of Pharmacy, Yokohama, Kanagawa 245-0066, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Shirako
authors.forename: Marina
authors.initials: M
authors.lastname: Kawasaki
authors.forename: Kotoe
authors.initials: K
authors.lastname: Komine
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kunisue
authors.forename: Masaru
authors.initials: M
authors.lastname: Terada
authors.forename: Chizuko
authors.initials: C
authors.lastname: Sasaki
authors.forename: Wataru
authors.initials: W
authors.lastname: Irie
authors.forename: Chikako
authors.initials: C
authors.lastname: Murakami
authors.forename: Keiko
authors.initials: K
authors.lastname: Tonooka
authors.forename: Koji
authors.initials: K
authors.lastname: Tomobe
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Shinozuka
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods; D053593:Forensic Toxicology / Q000379:methods; D006801:Humans; D000077239:Meloxicam; D015394:Molecular Structure; D010894:Piroxicam / Q000031:analogs & derivatives / Q000097:blood / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods; D013843:Thiazines / Q000097:blood / Q000737:chemistry; D013844:Thiazoles / Q000097:blood / Q000737:chemistry
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D013843:Thiazines; D013844:Thiazoles; C065946:ampiroxicam; D010894:Piroxicam; C059451:lornoxicam; D000077239:Meloxicam; C032801:tenoxicam