title: Low expression of nucleus accumbens-associated protein 1 predicts poor prognosis for patients with pancreatic ductal adenocarcinoma.
abstract: Nucleus accumbens-associated protein 1 (NAC1) is overexpressed in various carcinomas including ovarian, cervical, breast, and pancreatic carcinomas. High expression of NAC1 is considered to have adverse effects on prognosis through negative regulation of growth arrest and DNA-damage-inducible 45-γ interacting protein 1 (GADD45GIP1) in ovarian and cervical carcinomas. In the present study, the expression of NAC1 in pancreatic ductal adenocarcinoma (PDA) was measured using immunohistochemistry and computer-assisted image analysis in order to investigate its correlation with various clinicopathological parameters and prognosis. Patients with low-NAC1 PDA had worse overall survival (P = 0.0010) and a shorter disease-free survival (P = 0.0036) than patients with high-NAC1 PDA. This was a clinical effect opposite to that reported in ovarian and cervical carcinomas. Furthermore, knockdown of NAC1 in pancreatic carcinoma cell lines did not increase expression of the GADD45GIP1 protein. These results indicate that the gene(s) regulated by NAC1 vary depending on the types of carcinoma or originating tissue, and that low expression of NAC1 predicts poor prognosis for patients with PDA.
authors.affiliation: Department of Digestive and General Surgery, Shimane University Faculty of Medicine, Izumo, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nishi
authors.forename: Riruke
authors.initials: R
authors.lastname: Maruyama
authors.forename: Takeshi
authors.initials: T
authors.lastname: Urano
authors.forename: Naomi
authors.initials: N
authors.lastname: Nakayama
authors.forename: Yasunari
authors.initials: Y
authors.lastname: Kawabata
authors.forename: Seiji
authors.initials: S
authors.lastname: Yano
authors.forename: Manabu
authors.initials: M
authors.lastname: Yoshida
authors.forename: Kentaro
authors.initials: K
authors.lastname: Nakayama
authors.forename: Kohji
authors.initials: K
authors.lastname: Miyazaki
authors.forename: Keizo
authors.initials: K
authors.lastname: Takenaga
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Tanaka
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Tajima
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D021441:Carcinoma, Pancreatic Ductal / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D018797:Cell Cycle Proteins / Q000096:biosynthesis; D045744:Cell Line, Tumor; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007090:Image Interpretation, Computer-Assisted; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis / Q000096:biosynthesis*; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000096:biosynthesis; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D011379:Prognosis; D034741:RNA, Small Interfering; D012097:Repressor Proteins / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; C476586:GADD45GIP1 protein, human; C516477:NACC1 protein, human; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D012097:Repressor Proteins