title: DNA methylation of RUNX3 in papillary thyroid cancer.
abstract: OBJECTIVE
  The relationship between Runt-related transcription factor 3 (RUNX3) gene inactivation and various solid tumors has been reported; however, little information is available about RUNX3 in thyroid cancers.
  
  
  METHODS
  We evaluated the DNA methylation of RUNX3 in 13 papillary thyroid cancer tissues and four thyroid cancer cell lines. Additionally, using reverse transcriptase-polymerase chain reaction, we analyzed RUNX3 gene expression in several thyroid cancer cell lines after treating with the demethylating agent 5-aza-2'-deoxycytidine (DAC).
  
  
  RESULTS
  RUNX3 was hypermethylated in many thyroid cancer cell lines and in 10 of the 12 papillary thyroid cancer tissues. Treatment with DAC increased the expression of RUNX3 in some thyroid cancer cell lines.
  
  
  CONCLUSIONS
  We suggest that RUNX3 is associated with thyroid carcinogenesis, and RUNX3 methylation is a potentially useful diagnostic marker for papillary thyroid cancer.
authors.affiliation: Department of Internal Medicine, Soonchunhyang University College of Medicine, Cheonan, Korea.
authors.forename: Hee Ja
authors.initials: HJ
authors.lastname: Ko
authors.forename: Bo Yeon
authors.initials: BY
authors.lastname: Kim
authors.forename: Chan Hee
authors.initials: CH
authors.lastname: Jung
authors.forename: Sung Wan
authors.initials: SW
authors.lastname: Chun
authors.forename: Ji Oh
authors.initials: JO
authors.lastname: Mok
authors.forename: Yeo Joo
authors.initials: YJ
authors.lastname: Kim
authors.forename: Hyeong Kyu
authors.initials: HK
authors.lastname: Park
authors.forename: Chul Hee
authors.initials: CH
authors.lastname: Kim
authors.forename: Sang Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Dong Won
authors.initials: DW
authors.lastname: Byun
authors.forename: Kyo Il
authors.initials: KI
authors.lastname: Suh
authors.forename: Myung Hi
authors.initials: MH
authors.lastname: Yoo
authors.forename: Sung Gu
authors.initials: SG
authors.lastname: Kang
mesh_terms: D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D014408:Biomarkers, Tumor / Q000235:genetics; D002277:Carcinoma / Q000235:genetics*; D002291:Carcinoma, Papillary; D045744:Cell Line, Tumor; D050677:Core Binding Factor Alpha 3 Subunit / Q000235:genetics*; D019175:DNA Methylation / Q000187:drug effects; D000077209:Decitabine; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D050677:Core Binding Factor Alpha 3 Subunit; C493729:Runx3 protein, human; D000077209:Decitabine; D001374:Azacitidine