title: Nucleation promoting factors regulate the expression and localization of Arp2/3 complex during meiosis of mouse oocytes.
abstract: The actin nucleation factor Arp2/3 complex is a main regulator of actin assembly and is involved in multiple processes like cell migration and adhesion, endocytosis, and the establishment of cell polarity in mitosis. Our previous work showed that the Arp2/3 complex was involved in the actin-mediated mammalian oocyte asymmetric division. However, the regulatory mechanisms and signaling pathway of Arp2/3 complex in meiosis is still unclear. In the present work, we identified that the nucleation promoting factors (NPFs) JMY and WAVE2 were necessary for the expression and localization of Arp2/3 complex in mouse oocytes. RNAi of both caused the degradation of actin cap intensity, indicating the roles of NPFs in the formation of actin cap. Moreover, JMY and WAVE2 RNAi decreased the expression of ARP2, a key component of Arp2/3 complex. However, knock down of Arp2/3 complex by Arpc2 and Arpc3 siRNA microinjection did not affect the expression and localization of JMY and WAVE2. Our results indicate that the NPFs, JMY and WAVE2, are upstream regulators of Arp2/3 complex in mammalian oocyte asymmetric division.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Qiao-Chu
authors.initials: QC
authors.lastname: Wang
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.forename: Xing
authors.initials: X
authors.lastname: Duan
authors.forename: Xiao-Xin
authors.initials: XX
authors.lastname: Dai
authors.forename: Teng
authors.initials: T
authors.lastname: Wang
authors.forename: Hong-Lin
authors.initials: HL
authors.lastname: Liu
authors.forename: Xiang-Shun
authors.initials: XS
authors.lastname: Cui
authors.forename: Nam-Hyung
authors.initials: NH
authors.lastname: Kim
authors.forename: Shao-Chen
authors.initials: SC
authors.lastname: Sun
mesh_terms: D051376:Actin-Related Protein 2-3 Complex / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D018797:Cell Cycle Proteins; D005260:Female; D005786:Gene Expression Regulation; D008540:Meiosis; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism*; D021381:Protein Transport; D034622:RNA Interference; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D051300:Wiskott-Aldrich Syndrome Protein Family / Q000235:genetics / Q000378:metabolism
chemical_list: D051376:Actin-Related Protein 2-3 Complex; D018797:Cell Cycle Proteins; C485677:Jmy protein, mouse; D009687:Nuclear Proteins; D015534:Trans-Activators; D051300:Wiskott-Aldrich Syndrome Protein Family