title: Proteomic analysis identifies a novel function for galectin-3 in the cell entry of parvovirus.
abstract: Cellular factors associated with the parvovirus minute virus of mice (MVM) during infection are thought to play important roles in the MVM life cycle but only a few of these have been identified. Here we used a proteomic-based approach in order to identify host-binding partners of MVM. Using purified MVM as bait for immunoprecipitation assays, a total of 150 proteins were identified in MVM immunoprecipitates by quantitative liquid chromatography-tandem mass spectrometry. Galectin-3 was one of six proteins showing a statistically significant enrichment across replicates. Small interfering RNA depletion studies revealed an important role for galectin-3 in MVM endocytosis and infectivity in LA9 mouse fibroblast cells. Galectin-3-depleted cells were less susceptible to MVM infection than control cells and showed a significant reduction of MVM cellular uptake, but not of MVM binding to the cell surface. Our results indicate an important role for galectin-3 in the cellular uptake of MVM. We propose that galectin-3 facilitates the access of MVM to its receptor(s) at the plasma membrane and in this way promotes MVM endocytosis.
authors.affiliation: Department of Zoology, University of British Columbia, 6270 University Boulevard, Vancouver, BC, Canada V6T 1Z4.
authors.forename: Pierre
authors.initials: P
authors.lastname: Garcin
authors.forename: Sarah
authors.initials: S
authors.lastname: Cohen
authors.forename: Sanne
authors.initials: S
authors.lastname: Terpstra
authors.forename: Isabelle
authors.initials: I
authors.lastname: Kelly
authors.forename: Leonard J
authors.initials: LJ
authors.lastname: Foster
authors.forename: Nelly
authors.initials: N
authors.lastname: Panté
mesh_terms: D000818:Animals; D004705:Endocytosis / Q000502:physiology; D037502:Galectin 3 / Q000502:physiology*; D051379:Mice; D008915:Minute Virus of Mice / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D040901:Proteomics; D011991:Receptors, Virus / Q000502:physiology*; D053586:Virus Internalization; D014779:Virus Replication / Q000235:genetics
chemical_list: D037502:Galectin 3; D011991:Receptors, Virus