title: Geodermatophilus saharensis sp. nov., isolated from sand of the Saharan desert in Chad.
abstract: A novel Gram-positive, aerobic, actinobacterial strain, CF5/5, was isolated from soil in the Sahara desert, Chad. It grew best at 20-35 °C and at pH 6.0-8.0 and with 0-4 % (w/v) NaCl, forming black-colored colonies. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The DNA G + C content was 75.9 mol%. The peptidoglycan contained meso-diaminopimelic acid; galactose and xylose were detected as diagnostic sugars. The main phospholipids were diphosphatidylglycerol, phosphatidylcholine, and phosphatidylinositol; MK-9(H(4)) was the dominant menaquinone. The major cellular fatty acids were: iso-C(16:0) and iso-C(15:0). The 16S rRNA gene showed 95.6-98.3 % sequence similarity with the other named members of the genus Geodermatophilus. Based on the polyphasic taxonomy data, the isolate is proposed to represent a novel species, Geodermatophilus saharensis with the type strain CF5/5(T) = DSM 45423 = CCUG 62813 = MTCC 11416.
authors.affiliation: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
authors.forename: M C
authors.initials: MC
authors.lastname: Montero-Calasanz
authors.forename: M
authors.initials: M
authors.lastname: Göker
authors.forename: G
authors.initials: G
authors.lastname: Pötter
authors.forename: M
authors.initials: M
authors.lastname: Rohde
authors.forename: C
authors.initials: C
authors.lastname: Spröer
authors.forename: P
authors.initials: P
authors.lastname: Schumann
authors.forename: A A
authors.initials: AA
authors.lastname: Gorbushina
authors.forename: H-P
authors.initials: HP
authors.lastname: Klenk
mesh_terms: D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D002473:Cell Wall / Q000737:chemistry; D002596:Chad; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012822:Silicon Dioxide; D012988:Soil Microbiology; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012822:Silicon Dioxide