title: Exploration of Vanilla pompona from the Peruvian Amazon as a potential source of vanilla essence: quantification of phenolics by HPLC-DAD.
abstract: This study provides the first chemical investigation of wild-harvested fruits of Vanilla pompona ssp. grandiflora (Lindl.) Soto-Arenas developed in their natural habitat in the Peruvian Amazon. Flowers were hand-pollinated and the resulting fruits were analysed at different developmental stages using an HPLC-DAD method validated for the quantification of glucovanillin and seven other compounds. The method showed satisfactory linearity (r(2)>0.9969), precision (coefficient of variation <2%), recoveries (70-100%), limit of detection (0.008-0.212 μg/ml), and limit of quantification (0.027-0.707 μg/ml). The evaluation of crude and enzyme-hydrolyzed Soxhlet-extracted samples confirmed the leading role of glucosides in fruit development. LC-ESI-MS studies corroborated the identities of four glucosides and seven aglycones, among them vanillin (5.7/100 g), 4-hydroxybenzyl alcohol (3.6/100 g), and anisyl alcohol (7.1/100 g) were found in high concentrations. The attractive flavor/aroma profile exhibited by wild V. pompona fruits supports studies focused on the development of this species as a specialty crop.
authors.affiliation: Pontificia Universidad Catolica del Peru, Departamento de Ciencias - Quimica, Av. Universitaria 1801, Lima 32, Peru. hmaruen@pucp.edu.pe
authors.forename: Helena
authors.initials: H
authors.lastname: Maruenda
authors.forename: Maria del Lujan
authors.initials: Mdel L
authors.lastname: Vico
authors.forename: J Ethan
authors.initials: JE
authors.lastname: Householder
authors.forename: John P
authors.initials: JP
authors.lastname: Janovec
authors.forename: Cristhian
authors.initials: C
authors.lastname: Cañari
authors.forename: Angelica
authors.initials: A
authors.lastname: Naka
authors.forename: Ana E
authors.initials: AE
authors.lastname: Gonzalez
mesh_terms: D001547:Benzaldehydes / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D013058:Mass Spectrometry; D010568:Peru; D010636:Phenols / Q000032:analysis* / Q000302:isolation & purification; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification; D031669:Vanilla / Q000737:chemistry*
chemical_list: D001547:Benzaldehydes; D010636:Phenols; D010936:Plant Extracts; C100058:vanillin