title: [Usefulness of CTCs in tumor drainage vein blood as a biomarker for prognosis in colorectal cancer patients with Dukes' stage B and C].
abstract: We aimed to clarify the clinical significance of circulating tumor cells (CTCs) in the tumor drainage vein blood of colorectal cancer(CRC) patients with Dukes' stage B and C. This study included 111 patients with Dukes' stage B and 86 patients with Dukes' stage C. We selected multiple genetic markers, including the cancer-associated marker (CEA), epithelial markers(CK19 and CK20), and cancer stem-like cell marker(CD133), and the mRNA levels of these genes were detected by quantitative real-time reverse transcription-polymerase chain reaction assays. In Kaplan-Meier survival curve analysis, overall survival(OS) and disease-free survival(DFS) of Dukes' stage B and C patients who were positive for CEA, CK19, CK20, and/or CD133 (CEA/CK/CD133) were significantly worse than that in patients who were negative for these markers. By Cox progression analysis, it was demonstrated that CEA/CK/CD133 mRNA in tumor drainage vein blood was an independent prognostic factor for OS and DFS in these patients. These results suggest that CEA/CK/CD133 mRNA detection in tumor drainage vein blood is a useful tool for the determination of high-risk CRC patients with Dukes' stage B and C who are in need of postoperative adjuvant therapy.
authors.affiliation: Dept. of Surgery, Teikyo University School of Medicine, Japan.
authors.forename: Ryu
authors.initials: R
authors.lastname: Shimada
authors.forename: Hisae
authors.initials: H
authors.lastname: Iinuma
authors.forename: Junko
authors.initials: J
authors.lastname: Tamura
authors.forename: Atsushi
authors.initials: A
authors.lastname: Horiuchi
authors.forename: Keijiro
authors.initials: K
authors.lastname: Nozawa
authors.forename: Soichiro
authors.initials: S
authors.lastname: Ishihara
authors.forename: Keiji
authors.initials: K
authors.lastname: Matsuda
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Watanabe
authors.forename: Yojiro
authors.initials: Y
authors.lastname: Hashiguchi
mesh_terms: D000328:Adult; D000368:Aged; D015179:Colorectal Neoplasms / Q000175:diagnosis*; D004322:Drainage; D006801:Humans; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating; D011379:Prognosis