title: Development and evaluation of nucleic acid-based techniques for an auxiliary diagnosis of invasive fungal infections in formalin-fixed and paraffin-embedded (FFPE) tissues.
abstract: Notwithstanding the advantages of providing definitive diagnoses, the identification of fungi based on histopathological determination can be difficult and may lead to a pit of diagnoses. Therefore, the establishment of an auxiliary diagnostic method for use in routine pathological laboratories is desirable and should improve the above situation. Our previous studies have shown the superiority of in situ hybridization (ISH) for the detection of pathogenic fungi in histological specimens. This review focuses on the usefulness of ISH in the detection and identification of pathogenic fungi from formalin-fixed and paraffin-embedded (FFPE) tissue sections, and provides an overview of ISH for the diagnosis of fungal infection and retrospective autopsy analysis using molecular procedures. Based on the above, peptide nucleic acid (PNA) probes were shown to be superior in terms of the detection of target fungi and useful since histopathological diagnosis has the potential danger of being incorrect in the identification of fungi. In conclusion, we wish to emphasize that histopathological diagnosis in combination with molecular methods such as ISH and polymerase chain reaction (PCR) of FFPE samples should enhance the accuracy of identification in relation to fungi.
authors.affiliation: Department of Surgical Pathology, Toho University School of Medicine, Tokyo, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Shinozaki
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Okubo
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sasai
authors.forename: Haruo
authors.initials: H
authors.lastname: Nakayama
authors.forename: Takao
authors.initials: T
authors.lastname: Ishiwatari
authors.forename: Somay
authors.initials: S
authors.lastname: Murayama
authors.forename: Naobumi
authors.initials: N
authors.lastname: Tochigi
authors.forename: Megumi
authors.initials: M
authors.lastname: Wakayama
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nemoto
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Shibuya
mesh_terms: D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D017403:In Situ Hybridization; D009181:Mycoses / Q000382:microbiology*; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D016707:Tissue Fixation
chemical_list: D005404:Fixatives; D005557:Formaldehyde