title: Kinetics of protein physicochemical changes induced by heating in meat using mimetic models: (1) relative effects of heat and oxidants.
abstract: Optimizing the nutritional quality of cooked meat needs a better understanding of the mechanisms responsible for protein changes induced by heating. The relative contributions of chemical and thermal effects on protein physicochemical changes were studied using meat models. Two models were tested: a basic model made of an aqueous suspension of myofibrillar proteins, and a complex model, in which oxidants were added in physiological concentrations. Various heating time-temperature combinations were applied to both models in the ranges 45-90 °C and 5-120 min. Protein oxidation was evaluated by carbonyl and free thiol contents. Conformational changes of proteins were assessed by measurements of surface hydrophobicity and aggregation. Carbonyl formation was weakly affected by the thermal process alone but exacerbated by oxidants. A synergistic effect of oxidants and heat treatments on protein oxidation was noted. Changes in protein hydrophobicity and aggregation were dominated by the thermal process.
authors.affiliation: INRA, UR370 Qualité des Produits Animaux, F-63122 Saint-Genès-Champanelle, France.
authors.forename: A
authors.initials: A
authors.lastname: Promeyrat
authors.forename: J D
authors.initials: JD
authors.lastname: Daudin
authors.forename: P
authors.initials: P
authors.lastname: Gatellier
mesh_terms: D000818:Animals; D003296:Cooking; D005260:Female; D006358:Hot Temperature; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D008460:Meat / Q000032:analysis*; D008956:Models, Chemical; D009124:Muscle Proteins / Q000737:chemistry*; D016877:Oxidants / Q000737:chemistry*; D010084:Oxidation-Reduction; D011817:Rabbits
chemical_list: D009124:Muscle Proteins; D016877:Oxidants