title: [Study on quality evaluation of Sparganii rhizoma by biopotency determination method].
abstract: OBJECTIVE
  To establish a method for determining anticoagulation potency of Sparganii Rhizoma, and evaluate the effect of Sparganii Rhizoma herbs from different producing areas on promoting blood circulation and removing blood stasis; and study the material basis of Sparganii Rhizoma through the correlation analysis on its anticoagulation potency, ferulic acid and total flavonoid content.
  
  
  METHODS
  The anticoagulation time of Sparganii Rhizoma from different producing areas with activeated partial thromboplastin time for their active extracts. Their biopotency was calculated by using the method of "parallel lines of dose effect" (3, 3). The degree of correlation between their anticoagulation potency and chemical constituents were calculated by using Pearson correlational analysis method.
  
  
  RESULTS
  Sparganii Rhizoma and is control drugs had a good linear relationship between dose and effect (Y = 172.76X - 193.39, R2 = 0.9955). The method had better accuracy (RSD 4.7%), repeatability (RSD 2.3%) and intermediate precision (RSD 5.4%), finding that the biopotency of Sparganii Rhizoma from different producing areas ranged between 52.33-238.58 U x g(-1), and all of them passed the test on reliability. The results of correlation analysis showed no remarkable relationship between the anticoagulation potency of Sparganii Rhizoma and the contents of the two chemical constituents.
  
  
  CONCLUSIONS
  This biopotency determination method established in the experiment can be used as one of approaches for qulaity evaluation on Sparganii Rhizoma.
authors.affiliation: Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China. xrays1987@163.com
authors.forename: Guang-yun
authors.initials: GY
authors.lastname: Chen
authors.forename: Qi-nan
authors.initials: QN
authors.lastname: Wu
authors.forename: Xin-sheng
authors.initials: XS
authors.lastname: Wang
authors.forename: Qiao-li
authors.initials: QL
authors.lastname: Liang
authors.forename: Xiao-xiao
authors.initials: XX
authors.lastname: He
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000737:chemistry* / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology*; D011817:Rabbits; D027343:Rhizome / Q000737:chemistry*; D032401:Typhaceae / Q000737:chemistry*
chemical_list: D000925:Anticoagulants; D004365:Drugs, Chinese Herbal