title: Expression profile analyses of human HCT-116 colon cancer cell line before and after serum induction.
abstract: Multi-exon genes may generate distinct isoforms in different conditions and exhibit versatile properties. Here we investigated the isoform-specific gene expression and the gene expression changes of without and with serum-induced human HCT-116 colon cancer cell lines. For these analyses, 4 transcriptome sequencing datasets were used and 2 replicates for each condition. We observed that ~73% of those expressed genes in four samples generated only one isoform, while ~27% encoded at least two isoforms. Our results show that human gene expression can exhibit great flexibility in alternative splicing. Those expressed genes generated ~1.4 isoforms for each gene across four samples on average. In addition, most of these expressed genes were expressed at moderate or low levels. We found that these four samples have similar patterns of their gene expression distributions. Furthermore, we also conducted the differential expression analysis between without and with serum-induced two conditions of these four samples. We detected that 123 genes were differentially expressed and 110 of them were up-regulated. Among those differentially expressed genes, we found intriguing phenomena that a portion of them could be clustered into different functional groups and some oncogenes and proto-oncogenes are differentially expressed.
authors.affiliation: Department of General Surgery, Sheng Jing Hospital of China Medical University, Shenyang City, Liaoning Province 110004, China.
authors.forename: Hongzhuan
authors.initials: H
authors.lastname: Yin
authors.forename: Zhaopeng
authors.initials: Z
authors.lastname: Yan
authors.forename: Yichao
authors.initials: Y
authors.lastname: Liang
authors.forename: Baolin
authors.initials: B
authors.lastname: Liu
authors.forename: Qi
authors.initials: Q
authors.lastname: Su
mesh_terms: D017398:Alternative Splicing; D003110:Colonic Neoplasms / Q000235:genetics; D003470:Culture Media; D045325:HCT116 Cells; D006801:Humans; D020033:Protein Isoforms / Q000235:genetics; D044967:Serum / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D003470:Culture Media; D020033:Protein Isoforms