title: Cladosporium cladosporioides XJ-AC03, an aconitine-producing endophytic fungus isolated from Aconitum leucostomum.
abstract: The endophytic fungus XJ-AC03, which was isolated from the healthy roots of Aconitum leucostomum, produced aconitine when grown in potato dextrose agar (PDA) medium. The presence of aconitine was confirmed by the chromatographic and spectroscopic analyses. The yield of aconitine was recorded as 236.4 μg/g by high performance liquid chromatography (HPLC). The mass spectrometry was shown to be identical to authentic aconitine. Further analysis with nuclear magnetic resonance (NMR) spectroscopy to show the chemical structure of the fungal aconitine indicated that the fungal aconitine produced an NMR spectrum identical to that of authentic aconitine. Strain XJ-AC03 was identified as Cladosporium cladosporioides by its characteristic culture morphology and ITS rDNA sequence analysis.
authors.affiliation: College of Animal Veterinary Medicine, Northwest A&F University, Yangling 712100, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Liang
authors.forename: Qinfan
authors.initials: Q
authors.lastname: Li
authors.forename: Xiangya
authors.initials: X
authors.lastname: Kong
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.forename: Yimin
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000157:Aconitine / Q000032:analysis / Q000378:metabolism*; D031841:Aconitum / Q000382:microbiology*; D002851:Chromatography, High Pressure Liquid; D002963:Cladosporium / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D060026:Endophytes / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D000157:Aconitine