title: The effects of non-ionic contrast medium on the hemorheology in vitro and in vivo.
abstract: Contrast media are the commonly used agents in radiology. However, because of their characteristics of high osmolality, high viscosity, and chemical toxicity, the administrations of contrast media have been shown to cause adverse effects especially on hemorheology in short time course. The present study is to find the effects of a non-ionic contrast medium, iopromide, on the hemorheology in long time course both in vitro and in vivo. For in vitro treatment, human peripheral blood samples were incubated with contrast medium at 37°C for 0.5, 1 and 2 h. For in vivo study, about 15 ml of contrast medium was injected into rabbits and blood samples were collected at 0.5, 2, 6, and 24 h after the bolus injection. Hemorheological parameters were examined. Results showed that hematocrit adjusted whole blood viscosity increased significantly at 1 h after in vitro treatment of contrast medium, while it decreased at 0.5 h and remained low till 6 h after bolus injection. Ektacytometer showed that erythrocyte deformability decreased to the lowest level at 2 h in vitro and it dropped at 0.5 h and resumed to normal after 2 h in vivo. Erythrocyte small deformation indices were reduced by contrast medium in both in vitro and in vivo studies. Erythrocyte orientation index was also reduced in in vivo study. Erythrocyte electrophoresis rates at all time points decreased but osmotic fragility did not change in both studies. These impaired hemorheological parameters may disturb the microcirculation and cause adverse effects in patients with kidney diseases.
authors.affiliation: Chengde Medical College, Chengde, Hebei Province, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Song
authors.affiliation: Emergency Department, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.
authors.forename: Xifu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Guiyang Medical College, Guiyang, Guizhou Province, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Clinical Laboraotory, First Central Hospital of Baoding, Baoding, Hebei Province, China.
authors.forename: Binbin
authors.initials: B
authors.lastname: Jia
authors.affiliation: Guiyang Medical College, Guiyang, Guizhou Province, China.
authors.forename: Qinni
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: Hemorheology Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
authors.forename: Weibo
authors.initials: W
authors.lastname: Ka
authors.affiliation: Hemorheology Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
authors.forename: Dagong
authors.initials: D
authors.lastname: Sun
authors.affiliation: Hemorheology Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
authors.forename: Zongyao
authors.initials: Z
authors.lastname: Wen
authors.affiliation: Chengde Medical College, Chengde, Hebei Province, China.
authors.forename: Lide
authors.initials: L
authors.lastname: Xie
authors.affiliation: Hemorheology Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
authors.forename: Weijuan
authors.initials: W
authors.lastname: Yao
mesh_terms: D000818:Animals; D001809:Blood Viscosity / Q000187:drug effects*; D003287:Contrast Media / Q000494:pharmacology*; D004907:Erythrocyte Deformability / Q000187:drug effects; D005260:Female; D018056:Hemorheology / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D011817:Rabbits
chemical_list: D003287:Contrast Media