title: Determination of volatile N-nitrosamines in meat products by microwave-assisted extraction coupled with dispersive micro solid-phase extraction and gas chromatography--chemical ionisation mass spectrometry.
abstract: A sensitive procedure, microwave-assisted extraction (MAE) coupled dispersive micro solid-phase extraction (D-μ-SPE), was developed to extract N-nitrosodimethylamine (NDMA) and other six volatile N-nitrosamines (NAms) from meat products. Parameters affecting the efficiency of MAE and D-μ-SPE were systematically investigated. For MAE, 5-g of a homogenised meat sample was extracted with 30 mL of a sodium hydroxide (0.025 M) solution at 100 °C for 10 min. The optimum D-μ-SPE conditions were immersing 100mg of Carboxen™ 1000 adsorbent in the MAE extract. After vigorously shaking for 30 min, the NAms were then desorbed by treatment with 200 μL of dichloromethane. A 10 μL aliquot was determined by gas chromatography with chemical ionisation mass spectrometry (GC-CI-MS) using the selected-ion-storage (SIS) mode. The limits of quantitation (LOQs) were 0.03-0.36 ng/g. Preliminary results revealed that NDMA was present in the highest concentration, ranging from 0.8 to 3.2 ng/g.
authors.affiliation: Department of Chemistry, National Central University, Chung-Li 320, Taiwan.
authors.forename: Man-Chun
authors.initials: MC
authors.lastname: Huang
authors.forename: Hsin-Chang
authors.initials: HC
authors.lastname: Chen
authors.forename: Ssu-Chieh
authors.initials: SC
authors.lastname: Fu
authors.forename: Wang-Hsien
authors.initials: WH
authors.lastname: Ding
mesh_terms: D000818:Animals; D004128:Dimethylnitrosamine / Q000032:analysis / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008461:Meat Products / Q000032:analysis*; D008872:Microwaves; D009602:Nitrosamines / Q000032:analysis* / Q000302:isolation & purification; D052617:Solid Phase Microextraction / Q000295:instrumentation / Q000379:methods*; D013552:Swine
chemical_list: D009602:Nitrosamines; D004128:Dimethylnitrosamine