title: A novel and sensitive method for the detection of enrofloxacin in food using time-resolved fluoroimmunoassay.
abstract: A time-resolved fluoroimmunoassay (TRFIA) technique is developed to detect enrofloxacin (ENR) contamination in food. By using ENR-ovalbumin, anti-ENR antibodies and europium-labeled goat anti-rabbit antibodies, we establish an indirect and competitive method for ENR-TRFIA. The sensitivity of the method is high, with a detection limit of 0.01 ng/mL. The tests show that the technique's sensitivity is 1 μg/kg in eel, pork and chicken, and 1 μg/L in honey. The detection range attained is 0.01-100 ng/mL and within the detection range the intra- and inter-batch coefficients of variation of the ENR-TRFIA method are 2.4% and 9.2%, respectively. The data obtained from eel samples by TRFIA and enzyme-linked immunoassay are in good agreement. The assay did not cross-react with other quinolones, which commonly exist in food. The study suggests that ENR-TRFIA is a simple, sensitive and economic method of screening large quantities of samples, and has good prospects for application.
authors.affiliation: Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine , Wuxi, Jiangsu , PR China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Bin
authors.forename: Zhang
authors.initials: Z
authors.lastname: Kai
authors.forename: Zhang
authors.initials: Z
authors.lastname: Jue
authors.forename: Wang
authors.initials: W
authors.lastname: Ke
authors.forename: Zhao
authors.initials: Z
authors.lastname: Lili
authors.forename: Jin
authors.initials: J
authors.lastname: Jian
authors.forename: Huang
authors.initials: H
authors.lastname: Biao
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D000077422:Enrofloxacin; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique / Q000379:methods*; D024841:Fluoroquinolones / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D057230:Limit of Detection
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D000077422:Enrofloxacin