title: Overexpression of LASP1 is associated with proliferation, migration             and invasion in esophageal squamous cell carcinoma.
abstract: LIM and SH3 protein 1 (LASP1) is an actin-binding protein which is overexpressed             in many types of cancers and plays important roles in cancer progression. however,             the role of LASP1 in esophageal squamous cell carcinoma (ESCC) is still unknown.             We sought to analyze the expression level of LASP1 in ESCC, and the role of LASP1             in the development of ESCC was further investigated. We evaluated the expression             levels of LASP1 in 89 ESCC tissues and two ESCC cell lines using quantitative             real-time polymerase chain reaction, western blotting and immunohistochemistry.             The effects of LASP1 depletion on tumor cell behavior were investigated using             gene transfection and small interfering RNA (siRNA) in ESCC cell lines in vitro.             The expression levels of LASP1 at the mRNA and protein levels were significantly             higher in ESCC tissues and ESCC cell lines compared to adjacent tissues. Immunohistochemistry             showed that LASP1 was localized in the cytoplasm and nuclei of tumor epithelia.             Silencing of LASP1 in ECA109 and KYSE510 cell lines signiﬁcantly inhibited cell             proliferation, migration and invasion when compared with the negative control             cells in vitro. LASP1 may play an important role in the pathogenesis of ESCC and             shows promise as a treatment target in ESCC.
authors.affiliation: Department of Cardiothoracic Surgery, The Second Xiangya Hospital of Central South University, Changsha, Hunan, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: He
authors.forename: Bangliang
authors.initials: B
authors.lastname: Yin
authors.forename: Baoxiang
authors.initials: B
authors.lastname: Wang
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Zhenkun
authors.initials: Z
authors.lastname: Xia
authors.forename: Jingqun
authors.initials: J
authors.lastname: Tang
authors.forename: Yunchang
authors.initials: Y
authors.lastname: Yuan
authors.forename: Xiang
authors.initials: X
authors.lastname: Feng
authors.forename: Ni
authors.initials: N
authors.lastname: Yin
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006801:Humans; D060588:LIM Domain Proteins / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; C096353:LASP1 protein, human; D060588:LIM Domain Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering