title: Addressing the role of microRNAs in reprogramming leaf growth during drought stress in Brachypodium distachyon.
abstract: Plant responses to drought are regulated by complex genetic and epigenetic networks leading to rapid reprogramming of plant growth. miRNAs have been widely indicated as key players in the regulation of growth and development. The role of miRNAs in drought response was investigated in young leaves of Brachypodium distachyon, a drought-tolerant monocot model species. Adopting an in vivo drought assay, shown to cause a dramatic reduction in leaf size, mostly due to reduced cell expansion, small RNA libraries were produced from proliferating and expanding leaf cells. Next-generation sequencing data were analyzed using an in-house bioinformatics pipeline allowing the identification of 66 annotated miRNA genes and 122 new high confidence predictions greatly expanding the number of known Brachypodium miRNAs. In addition, we identified four TAS3 loci and a large number of siRNA-producing loci that show characteristics suggesting that they may represent young miRNA genes. Most miRNAs showed a high expression level, consistent with their involvement in early leaf development and cell identity. Proliferating and expanding leaf cells respond differently to drought treatment and differential expression analyses suggest novel evidence for an miRNA regulatory network controlling cell division in both normal and stressed conditions and demonstrate that drought triggers a genetic reprogramming of leaf growth in which miRNAs are deeply involved.
authors.affiliation: Institute of Life Sciences, Scuola Superiore Sant'Anna, Piazza Martiri della Libertà 33, 56127 Pisa, Italy.
authors.forename: Edoardo
authors.initials: E
authors.lastname: Bertolini
authors.forename: Wim
authors.initials: W
authors.lastname: Verelst
authors.forename: David Stephen
authors.initials: DS
authors.lastname: Horner
authors.forename: Luca
authors.initials: L
authors.lastname: Gianfranceschi
authors.forename: Viviana
authors.initials: V
authors.lastname: Piccolo
authors.forename: Dirk
authors.initials: D
authors.lastname: Inzé
authors.forename: Mario Enrico
authors.initials: ME
authors.lastname: Pè
authors.forename: Erica
authors.initials: E
authors.lastname: Mica
mesh_terms: D001483:Base Sequence; D058431:Brachypodium / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D002455:Cell Division / Q000235:genetics; D017124:Conserved Sequence; D055864:Droughts; D056426:Genetic Loci / Q000235:genetics; D023281:Genomics; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000235:genetics*; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development*; D018749:RNA, Plant / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant