title: The microRNA-27a: ZBTB10-specificity protein pathway is involved in             follicle stimulating hormone-induced VEGF, Cox2 and survivin expression in ovarian             epithelial cancer cells.
abstract: Previously, we demonstrated that follicle stimulating hormone (FSH) enhanced             VEGF expression and facilitated ovarian cancer angiogenesis via the PI3K/AKT signaling             pathway. In this study, we further investigated the involvement of microRNA-27a:             ZBTB10‑specificity protein pathway in the mechanism of FSH-induced VEGF, Cox2             and survivin expression. Treatment with FSH resulted in significant increase in             the expression of VEGF, Cox2, survivin, Sp1 proteins and microRNA-27a in a dose-dependent             manner, whereas reverse protein expression pattern was observed in ZBTB10. Downregulation             of microRNA-27a using antisense microRNA-27a blocked FSH-induced VEGF, Cox2 and             survivin expression. Overexpression of ZBTB10 also attenuated the FSH-induced             expression of these molecules. The enhanced expression of VEGF, Cox2 and survivin             was also abolished by knocking down Sp1 using small interfering RNA. Collectively,             these results indicated that stimulation of ovarian cancer cell VEGF, Cox2 and             survivin expression by FSH involves the microRNA‑27a: ZBTB10-specificity protein             pathway.
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Jiao Tong University                     Affiliated First People's Hospital, Shanghai, P.R. China.
authors.forename: Yunli
authors.initials: Y
authors.lastname: Lai
authors.forename: Xuerong
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhenbo
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yuyan
authors.initials: Y
authors.lastname: Shu
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Luo
authors.forename: Yongbin
authors.initials: Y
authors.lastname: Yang
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Wang
authors.forename: Guoping
authors.initials: G
authors.lastname: Yang
authors.forename: Linxia
authors.initials: L
authors.lastname: Li
authors.forename: Youji
authors.initials: Y
authors.lastname: Feng
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins; D000077216:Carcinoma, Ovarian Epithelial; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000096:biosynthesis*; D015536:Down-Regulation; D005260:Female; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000096:biosynthesis*; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics* / Q000473:pathology; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000378:metabolism; D015398:Signal Transduction; D000077022:Survivin; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis*
chemical_list: D051017:Apoptosis Regulatory Proteins; C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; C531388:MIRN27 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D012097:Repressor Proteins; D000077022:Survivin; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C559929:ZBTB10 protein, human; D005640:Follicle Stimulating Hormone; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D019869:Phosphatidylinositol 3-Kinases