title: [Preparation and applications of a supported liquid-liquid extraction column with a composite diatomite material].
abstract: A rapid and special supported liquid-liquid extraction (SLE) column was developed with a composite diatomite material. The SLE column was evaluated by high performance liquid chromatography (HPLC) with acidic, neutral and alkaline compounds dissolved in water. Furthermore, some real complex samples were also analyzed by HPLC with the SLE method. The recoveries of benzoic acid (acidic), p-nitroaniline (alkaline) and 4-hydroxy-benzoic methyl ester (neutral) treated by the SLE column were 90.6%, 98.1% and 97.7%. However, the recoveries of the three compounds treated by traditional liquid-liquid extraction (LLE) method were 71.9%, 81.9% and 83.9%. The results showed that the SLE technique had higher recoveries than the traditional LLE method. The spiked recoveries of the complex samples, such as benzoic acid in Sprite and dexamethasone acetate, chlorphenamine maleate, indomethacin in bovine serum, were between 80% and 110% and the relative standard deviations (RSDs) were less than 15%. For biological specimen, the results could be accepted. Meantime, many disadvantages associated with traditional LLE method, such as emulsion formation, didn't occur using SLE column. The SLE column technique is a good sample preparation method with many advantages, such as rapid, simple, robust, easily automated, high recovery and high-throughput, which would be widely used in the future.
authors.affiliation: Tianjin Key Laboratory for Modern Drug Delivery &High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Bao
authors.forename: Zhishuang
authors.initials: Z
authors.lastname: Ma
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.forename: Yongzun
authors.initials: Y
authors.lastname: Wang
authors.forename: Youxin
authors.initials: Y
authors.lastname: Li
mesh_terms: D000814:Aniline Compounds / Q000032:analysis / Q000302:isolation & purification; D019817:Benzoic Acid / Q000032:analysis / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003907:Dexamethasone / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification; D007692:Diatomaceous Earth / Q000737:chemistry*; D007213:Indomethacin / Q000032:analysis / Q000302:isolation & purification; D059625:Liquid-Liquid Extraction / Q000379:methods*
chemical_list: D000814:Aniline Compounds; C008625:nitroaniline; D007692:Diatomaceous Earth; C033787:diatomite; D003907:Dexamethasone; D019817:Benzoic Acid; C018038:dexamethasone acetate; D007213:Indomethacin