title: Selection of Ceratitis capitata (Diptera: Tephritidae) specific recombinant monoclonal phage display antibodies for prey detection analysis.
abstract: Several recombinant antibodies against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), one of the most important pests in agriculture worldwide, were selected for the first time from a commercial phage display library of human scFv antibodies. The specificity and sensitivity of the selected recombinant antibodies were compared with that of a rabbit polyclonal serum raised in parallel using a wide range of arthropod species as controls. The selected recombinant monoclonal antibodies had a similar or greater specificity when compared with classical monoclonal antibodies. The selected recombinant antibodies were successfully used to detect the target antigen in the gut of predators and the scFv antibodies were sequenced and compared. These results demonstrate the potential for recombinant scFv antibodies to be used as an alternative to the classical monoclonal antibodies or even molecular probes in the post-mortem analysis studies of generalist predators.
authors.affiliation: Department of Environmental Biology, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain.
authors.forename: César
authors.initials: C
authors.lastname: Monzó
authors.forename: Alberto
authors.initials: A
authors.lastname: Urbaneja
authors.forename: Miguel
authors.initials: M
authors.lastname: Ximénez-Embún
authors.forename: Julia
authors.initials: J
authors.lastname: García-Fernández
authors.forename: José Luis
authors.initials: JL
authors.lastname: García
authors.forename: Pedro
authors.initials: P
authors.lastname: Castañera
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000941:Antigens / Q000378:metabolism; D033622:Ceratitis capitata / Q000276:immunology* / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D019151:Peptide Library; D010935:Plant Diseases; D011235:Predatory Behavior; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry; D012680:Sensitivity and Specificity; D017386:Sequence Homology, Amino Acid; D057127:Single-Chain Antibodies / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D019151:Peptide Library; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies