title: Prevalence of low-risk HPV types and genital warts in women born 1988/89 or 1983/84 -results of WOLVES, a population-based epidemiological study in Wolfsburg, Germany.
abstract: BACKGROUND
  Wolfsburg HPV Epidemiological Study (WOLVES) is a population-based cohort study on HPV infections and associated diseases in the pre-vaccination era in young women in Wolfsburg, Germany.
  
  
  METHODS
  Women born 1983/84 or 1988/89 were invited to participate. Participants were recruited in gynecology practices, and completed a questionnaire with socioeconomic, sexual and medical data including vaccination status. Pelvic examination with Pap smear and HPV testing (HC2 = Hybrid Capture 2) was obligatory. HC2-positive and 10% of HC2-negative samples were tested for specific HPV types with SPF-10-PCR, and in inconclusive cases with DNA sequencing. Women with genital warts (GW) and those with atypical Pap smears were transferred for colposcopy. GWs were classified as typical condylomata acuminata (TCA), flat condyloma (FC) and seborrheic wart-like (SWL).
  
  
  RESULTS
  In total, 1258 subjects were recruited from the target population of 2850 (44.1%). Overall the prevalence of HC2 low-risk (LR) types was 8.5%. HPV6 was the most frequent LR type (2.1%), followed by HPV42 (1.1%), HPV11 and HPV44 (each 0.4%). LiPA showed a low sensitivity for HPV types 42, 90 and 91, which were detected only by HC2 and HPV sequencing. Nine women (0.7%) were transferred with incident GW: five TCA, two FC and two SWL. All TCA were associated with HPV6 in corresponding cervical swabs and warts. Tissues of SWL contained HPV6 (n = 1) and HPV16 (n = 1). The cumulative life-risk for GW was 1.4% in the 1988/89 and 4.8% in the 1983/84 cohort. Eight of 107 HC2-LR + and five of nine cases of GW had concomitant abnormal Pap smears. All CIN lesions could be linked to high-risk HPV types but borderline and low-grade abnormal smears were explained by vaginal and cervical TCA in four cases.
  
  
  CONCLUSIONS
  HC2 was a specific test for the detection of established and potential LR types. In this first WOLVES analysis, HPV6 was the most frequent HPV type and the single LR type linked to disease. The observed GW incidence of 715 per 100,000 fits well with estimates of healthcare providers. Although life risks for GW were lower than in Scandinavian analyses, the societal burden within the WOLVES populations was considerable.
authors.affiliation: Klinikum Wolfsburg, Frauenklinik, Schwerpunkt gynäkologische Onkologie, Sauerbruchstr, 7, 38440, Wolfsburg, Germany. k.u.petry@klinikum.wolfsburg.de
authors.forename: Karl Ulrich
authors.initials: KU
authors.lastname: Petry
authors.forename: Alexander
authors.initials: A
authors.lastname: Luyten
authors.forename: Annika
authors.initials: A
authors.lastname: Justus
authors.forename: Angelika
authors.initials: A
authors.lastname: Iftner
authors.forename: Sarah
authors.initials: S
authors.lastname: Strehlke
authors.forename: Renate
authors.initials: R
authors.lastname: Schulze-Rath
authors.forename: Thomas
authors.initials: T
authors.lastname: Iftner
mesh_terms: D015331:Cohort Studies; D003127:Colposcopy; D003218:Condylomata Acuminata / Q000453:epidemiology* / Q000821:virology*; D005260:Female; D005858:Germany / Q000453:epidemiology; D006801:Humans; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D011795:Surveys and Questionnaires; D014626:Vaginal Smears; D055815:Young Adult