title: Chitosan-coated PLGA nanoparticles: a sustained drug release strategy for cell cultures.
abstract: A recently patented one-step methodology was used for the formulation of chitosan (CS) coated polylactic-co-glycolic acid (PLGA) nanoparticles containing dexamethasone (DXM) as a model drug. SEM investigations showed that nanoparticles (NPs) were spherical in shape with smooth surface. CS coating switched NPs ζ-potential from negative to positive, without modifying particle size distribution. Moreover, CS coating allowed a significant modulation of in vitro drug release, providing a sustained drug delivery in cultured cells. The uptake of fluorescent CS-coated PLGA NPs by hepatocytes (C3A) and fibroblasts (3T6) as well as the fate of internalized NPs were investigated by confocal microscopy. 3T6 and C3A cells were treated with DXM-loaded NPs and experiments were addressed to analyze the specific cell response to DXM, in order to evaluate its functional efficiency in comparison with conventional addition to culture medium. CS-coating of DXM loaded PLGA NPs allowed their uptake by cultured cells without inducing cytotoxicity.
authors.affiliation: Department of Chemistry, Sapienza Università of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Chronopoulou
authors.forename: Mara
authors.initials: M
authors.lastname: Massimi
authors.forename: Maria Federica
authors.initials: MF
authors.lastname: Giardi
authors.forename: Cesare
authors.initials: C
authors.lastname: Cametti
authors.forename: Laura Conti
authors.initials: LC
authors.lastname: Devirgiliis
authors.forename: Mariella
authors.initials: M
authors.lastname: Dentini
authors.forename: Cleofe
authors.initials: C
authors.lastname: Palocci
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D051544:Cytochrome P-450 CYP3A / Q000096:biosynthesis; D003692:Delayed-Action Preparations / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology; D004705:Endocytosis / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D022781:Hepatocytes / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009995:Osmosis / Q000187:drug effects; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D055672:Static Electricity; D013997:Time Factors
chemical_list: D020099:Coated Materials, Biocompatible; D003692:Delayed-Action Preparations; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003907:Dexamethasone; D048271:Chitosan; D051544:Cytochrome P-450 CYP3A