title: Four-year experience with simultaneous culture and Shiga toxin testing for detection of Shiga toxin-producing Escherichia coli in stool samples.
abstract: We report our experience with universal Shiga toxin-producing Escherichia coli (STEC) screening using culture and Shiga toxin antigen testing over 4 years. Twelve cases were detected-8 detected by both culture and Shiga toxin immunochromatographic assay (IA), 3 by culture, and 1 by IA only. The addition of Shiga toxin testing is of questionable benefit over culture alone for detection of STEC in areas of low prevalence.
authors.affiliation: Department of Pathology, Wake Forest School of Medicine, Winston-Salem, North Carolina, USA.
authors.forename: Jennifer S
authors.initials: JS
authors.lastname: Woo
authors.forename: Elizabeth L
authors.initials: EL
authors.lastname: Palavecino
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001431:Bacteriological Techniques / Q000379:methods*; D018929:Cell Culture Techniques / Q000379:methods; D002648:Child; D002675:Child, Preschool; D002846:Chromatography, Affinity / Q000379:methods*; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008403:Mass Screening / Q000379:methods; D008875:Middle Aged; D022621:Shiga Toxin / Q000032:analysis*; D054323:Shiga-Toxigenic Escherichia coli / Q000302:isolation & purification*; D055815:Young Adult
chemical_list: D022621:Shiga Toxin