title: Factor V Leiden mutation is not a predisposing factor for acute coronary syndromes.
abstract: BACKGROUND
  The prevalence of Coronary artery disease (CAD) in India has increased considerably over the past few years and could become the number one killer disease if interventions are not done. Factor V Leiden (FVL) mutation and FII G20210A polymorphism are two recently described genetic factors with a propensity towards venous thrombosis. This warrants the investigations for thrombophilia in myocardial infarction patients in India.
  
  
  METHODS
  The study cohort consisted of 51 patients aged below 50 years presenting with acute coronary syndromes. In both patient group and normal individuals the major risk factors Protein C deficiency, Protein S deficiency, anticardiolipin antibodies, Fibrinogen and Lipoprotein [a] were studied. Factor V Leiden (FVL) G1691A mutation in both control and patient group was looked by using Polymerase chain reaction (PCR) followed by sequencing of the PCR products.
  
  
  RESULTS
  Our results indicated significantly higher levels of anticardiolipin antibodies and fibrinogen in the patients and absence of FVL (G1691A) mutation in our study cohort. One of the patients (H5) showed insertion of an extra A nucleotide in exon 10 of the Factor V gene resulting in frame shift mutation in this patient.
  
  
  CONCLUSIONS
  The results of present study showed absence of FVL mutation in our population. However, there is a need to confirm the above findings on patients from different populations from different parts of the country. The insertion of an extra A in exon 10 in the patient needs to be ascertained to confirm that it is one of its kinds or is prevalent in the population.
authors.affiliation: Department of Cardiology, Sri Venkateswara Institute of Medical Sciences & University, Andhra Pradesh, India.
authors.forename: G
authors.initials: G
authors.lastname: Himabindu
authors.forename: D
authors.initials: D
authors.lastname: Rajasekhar
authors.forename: K
authors.initials: K
authors.lastname: Latheef
authors.forename: P V G K
authors.initials: PV
authors.lastname: Sarma
authors.forename: V
authors.initials: V
authors.lastname: Vanajakshamma
authors.forename: Abhijit
authors.initials: A
authors.lastname: Chaudhury
authors.forename: Aparna R
authors.initials: AR
authors.lastname: Bitla
mesh_terms: D054058:Acute Coronary Syndrome / Q000097:blood / Q000235:genetics*; D000328:Adult; D016009:Chi-Square Distribution; D005165:Factor V / Q000235:genetics*; D005260:Female; D016368:Frameshift Mutation; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012044:Regression Analysis; D012307:Risk Factors; D018709:Statistics, Nonparametric; D019851:Thrombophilia / Q000235:genetics
chemical_list: C095381:factor V Leiden; D005165:Factor V