title: [Detection and clinical significance of FLT3-ITD gene mutation in patients with acute myeloid leukemia].
abstract: This study was aimed to evaluate the prevalence of FT3-ITD mutation in patients with acute myeloid leukemia (AML) and its significance for prognosis. The bone marrow of 216 patients with AML were detected by using PCR and R-banding methods. On basis of patients' clinical data the clinical feature and prognosis were analyzed. The results showed that in 216 AML patients incidence of FLT3-ITD mutation was 20.83%. FLT3-ITD mutation was present in 24% patients with normal karyotype while in 12.5% patients with abnormal karyotype. The FLT3-ITD positive patients had more white blood cells in peripheral blood and leukemia cells in bone marrow (P < 0.01). The free-disease survival and overall survival time were shorter in FLT3-ITD positive cases than that in FLT3-ITD negative cases (P < 0.05). It is concluded that FLT3-ITD mutation positive patients have more white blood cells in peripheral blood and leukemia cells in bone marrow. The FLT3-ITD mutation may be a danger factor in the AML patients with normal karyotype. It may be helpful for evaluating prognosis and guiding individual treatment of AML.
authors.affiliation: Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Xu
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.forename: Yi
authors.initials: Y
authors.lastname: Ding
authors.forename: Jun-Zhong
authors.initials: JZ
authors.lastname: Sun
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Yu
mesh_terms: D000328:Adult; D004252:DNA Mutational Analysis; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D059785:Karyotype; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D009154:Mutation; D011379:Prognosis; D051941:fms-Like Tyrosine Kinase 3 / Q000235:genetics*
chemical_list: C497970:FLT3 protein, human; D051941:fms-Like Tyrosine Kinase 3