title: Quantification of Candida albicans by flow cytometry using TO-PRO(®)-3 iodide as a single-stain viability dye.
abstract: This study investigated a flow cytometric, single-stain approach to quantify Candida albicans as an alternative for the standard viable plate count. TO-PRO(®)-3 iodide allows an excellent distinction between viable and dead cells. Both quantification methods show a high degree of correlation.
authors.affiliation: Laboratory of Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, B-2610 Wilrijk, Belgium.
authors.forename: Monique
authors.initials: M
authors.lastname: Kerstens
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Boulet
authors.forename: Isabel
authors.initials: I
authors.lastname: Pintelon
authors.forename: Mario
authors.initials: M
authors.lastname: Hellings
authors.forename: Liesbeth
authors.initials: L
authors.lastname: Voeten
authors.forename: Peter
authors.initials: P
authors.lastname: Delputte
authors.forename: Louis
authors.initials: L
authors.lastname: Maes
authors.forename: Paul
authors.initials: P
authors.lastname: Cos
mesh_terms: D002176:Candida albicans / Q000302:isolation & purification*; D002232:Carbocyanines / Q000378:metabolism*; D015169:Colony Count, Microbial / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D050296:Microbial Viability; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D002232:Carbocyanines; C098830:TO-PRO-3