title: Molecular identification of Paragonimus species by DNA pyrosequencing technology.
abstract: DNA pyrosequencing for PCR amplicons is an attractive strategy for the identification of microorganisms because of its short time performance for large number of samples. In this study, the primers targeting the fragment of ITS2 region of nuclear ribosomal RNA gene were newly developed for pyrosequencing-based identification of 6 Paragonimus species, Paragonimus bangkokensis, Paragonimus harinasutai, Paragonimus heterotremus, Paragonimus macrorchis, Paragonimus siamensis and Paragonimus westermani. Pyrosequencing determination of 39 nucleotides of partial ITS2 region could discriminate 6 Paragonimus species, and could also detect intra-species genetic variation of P. macrorchis. This DNA pyrosequencing-based identification can be a valuable tool to improve species-level identification of Paragonimus in the endemic areas.
authors.affiliation: Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Chairat
authors.initials: C
authors.lastname: Tantrawatpan
authors.forename: Pewpan M
authors.initials: PM
authors.lastname: Intapan
authors.forename: Penchom
authors.initials: P
authors.lastname: Janwan
authors.forename: Oranuch
authors.initials: O
authors.lastname: Sanpool
authors.forename: Viraphong
authors.initials: V
authors.lastname: Lulitanond
authors.forename: Chutatip
authors.initials: C
authors.lastname: Srichantaratsamee
authors.forename: Witthaya
authors.initials: W
authors.lastname: Anamnart
authors.forename: Wanchai
authors.initials: W
authors.lastname: Maleewong
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D010237:Paragonimiasis / Q000469:parasitology*; D010238:Paragonimus / Q000235:genetics / Q000302:isolation & purification*; D048492:Paragonimus westermani / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013997:Time Factors
chemical_list: D017931:DNA Primers; D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer