title: ¹H, ¹³C and ¹⁵N assignments of the four N-terminal domains of human fibrillin-1.
abstract: Fibrillins are extracellular, disulphide-rich glycoproteins that form 10-12 nm diameter microfibrils in connective tissues. They are found in the majority of higher animals, from jellyfish to humans. Fibrillin microfibrils confer properties of elasticity and strength on connective tissue and regulate growth factor availability in the extracellular matrix (ECM). Mutations in FBN1, the human gene encoding the fibrillin-1 isoform, are linked to several inherited connective tissue disorders. The fibrillin-1 N-terminus forms many functionally-important interactions, both with other fibrillin molecules and various ECM components. In particular, the first four domains, the fibrillin unique N-terminal (FUN) and three epidermal growth factor (EGF)-like domains (FUN-EGF3), are implicated in microfibril assembly and growth factor sequestration. The structure of these domains, which comprise 134 residues, is unknown. We have produced a recombinant fragment corresponding to this region of human fibrillin-1. Here, we report (1)H, (13)C and (15)N resonance assignments of the FUN-EGF3 fragment. Assignments will facilitate structure determination, analysis of interdomain dynamics and the mapping of interaction surfaces.
authors.affiliation: Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.
authors.forename: David A
authors.initials: DA
authors.lastname: Yadin
authors.forename: Ian B
authors.initials: IB
authors.lastname: Robertson
authors.forename: Sacha A
authors.initials: SA
authors.lastname: Jensen
authors.forename: Penny A
authors.initials: PA
authors.lastname: Handford
authors.forename: Christina
authors.initials: C
authors.lastname: Redfield
mesh_terms: D002247:Carbon Isotopes; D000071838:Fibrillin-1; D000071837:Fibrillins; D006801:Humans; D006859:Hydrogen; D008840:Microfilament Proteins / Q000737:chemistry*; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D017434:Protein Structure, Tertiary; D054392:Adipokines
chemical_list: D002247:Carbon Isotopes; D000071838:Fibrillin-1; D000071837:Fibrillins; D006859:Hydrogen; D008840:Microfilament Proteins; D009587:Nitrogen Isotopes; C000605722:FBN1 protein, human; D054392:Adipokines