title: Protein domain histochemistry (PDH): binding of the carbohydrate recognition domain (CRD) of recombinant human glycoreceptor CLEC10A (CD301) to formalin-fixed, paraffin-embedded breast cancer tissues.
abstract: Specialized protein domains bind to posttranslational modifications (PTMs) of proteins, such as phosphorylation or glycosylation. When such PTM-binding protein domains are used as analytical tools, the functional states of cells and tissues can be determined with high precision. Here, we describe the use of recombinant CLEC10A (CD301), a human glycoreceptor of the C-type lectin family, for the detection of ligands in sections from formalin-fixed, paraffin-embedded normal and cancerous mammary tissues. A construct, in which part of the carbohydrate recognition domain (CRD) was deleted, was used as a negative control. In comparison to normal mammary glands, a pronounced staining of tumor tissues was observed. Because the construct with the truncated CRD did not show any tissue staining, the binding of the wild-type glycoreceptor can be attributed to its carbohydrate recognition domain. To distinguish our novel approach from immunohistochemistry, we propose the designation "protein domain histochemistry" (PDH).
authors.affiliation: Department of Clinical Chemistry, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Nollau
authors.forename: Gerrit
authors.initials: G
authors.lastname: Wolters-Eisfeld
authors.forename: Naghmeh
authors.initials: N
authors.lastname: Mortezai
authors.forename: Anna-Katharina
authors.initials: AK
authors.lastname: Kurze
authors.forename: Birgit
authors.initials: B
authors.lastname: Klampe
authors.forename: Annegret
authors.initials: A
authors.lastname: Debus
authors.forename: Maximilian
authors.initials: M
authors.lastname: Bockhorn
authors.forename: Axel
authors.initials: A
authors.lastname: Niendorf
authors.forename: Christoph
authors.initials: C
authors.lastname: Wagener
mesh_terms: D001940:Breast / Q000473:pathology*; D001943:Breast Neoplasms / Q000175:diagnosis / Q000473:pathology*; D003001:Cloning, Molecular; D005260:Female; D057809:HEK293 Cells; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D037181:Lectins, C-Type / Q000032:analysis* / Q000378:metabolism*; D016612:Paraffin Embedding / Q000379:methods; D011134:Polysaccharides / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000032:analysis / Q000378:metabolism; D016707:Tissue Fixation / Q000379:methods
chemical_list: C545222:CLEC10A protein, human; D037181:Lectins, C-Type; D011134:Polysaccharides; D011994:Recombinant Proteins