title: Pattern of HPV infection in basal cell carcinoma and in perilesional skin biopsies from immunocompetent patients.
abstract: BACKGROUND
  The association between human papillomavirus (HPV) infection and non-melanoma skin cancers (NMSCs) such as squamous cell carcinoma (SCC) and basal cell carcinoma (BCC) is not yet fully understood. We analysed the prevalence and spectrum of cutaneous beta-HPV types and mucosal/genital HPV types in paired biopsies (tumour and corresponding perilesional skin) obtained from 50 BCC immunocompetent patients. A small group of SCC patients (n=9) was also included. We also evaluated some previously postulated risk factors for HPV infection in NMSC patients.
  
  
  RESULTS
  All biopsies were negative for mucosal/genital HPV types. Overall, beta-HPV DNA was detected more often in SCC compared to BCC patients (78% vs 55% of total samples). The frequency of infection increased with the patient's age [OR=4.88 (95% CI 1.29-18.39)]. There was no significant correlation between beta-HPV positivity and sex, skin type and UV exposure. The prevalence of beta-HPV species 1 types was significantly higher than those belonging to other beta-HPV species in biopsies from BCC (p=0.022) but not from SCC subjects (p=0.091). There was no significant difference in the overall prevalence of beta-HPV infection and the number of viral types between tumour lesions and perilesional skin. BCC samples were significantly more likely to be infected with beta-HPV species 1 types compared to perilesional skin (p=0.036) and showed a higher frequency of mixed infections (p=0.028).
  
  
  CONCLUSIONS
  These findings demonstrate that beta-HPV types belonging to species 1 are the most common HPV types detected in the skin of BCC patients. Moreover beta-1-HPV types and mixed infections are significantly more frequent in tumour samples than in healthy perilesional skin. Our results suggest that beta-1-HPVs as well as co-infection with more than one viral type could be important in NMSC and in particular in BCC.Further studies aimed to compare the biological activity of viral types in tumours and in healthy skin (viral replication and expression, interference of infection with cellular functions) are necessary to understand the role of HPV infection in skin cancer.
authors.affiliation: Department of Public Health, University of Florence, Viale Morgagni 48, Florence, 50134, Italy. krystyna.zakrzewska@unifi.it
authors.forename: Krystyna
authors.initials: K
authors.lastname: Zakrzewska
authors.forename: Elisa
authors.initials: E
authors.lastname: Regalbuto
authors.forename: Federica
authors.initials: F
authors.lastname: Pierucci
authors.forename: Rosaria
authors.initials: R
authors.lastname: Arvia
authors.forename: Sandra
authors.initials: S
authors.lastname: Mazzoli
authors.forename: Alessia
authors.initials: A
authors.lastname: Gori
authors.forename: Vincenzo
authors.initials: V
authors.lastname: de Giorgi
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D052686:Betapapillomavirus / Q000145:classification / Q000235:genetics; D001706:Biopsy; D002280:Basal Cell Carcinoma / Q000453:epidemiology / Q000473:pathology* / Q000821:virology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000473:pathology* / Q000821:virology; D015995:Prevalence; D012307:Risk Factors; D012867:Skin / Q000473:pathology; D012878:Skin Neoplasms / Q000453:epidemiology / Q000473:pathology* / Q000821:virology*; D014412:Tumor Virus Infections / Q000453:epidemiology / Q000473:pathology / Q000821:virology; D019562:Viral Load