title: Oceanicola litoreus sp. nov., an alphaproteobacterium isolated from the seashore sediment.
abstract: A Gram-negative, non-motile, facultatively aerobic and rod-shaped bacterial strain, designated M-M22(T), was isolated from seashore sediment on the southern coast in Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain M-M22(T) was observed to grow optimally at pH 7.0-7.5, at 30 °C and in the presence of 2 % (w/v) NaCl. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain M-M22(T) fell within the clade comprising the type strains of Oceanicola species, except Oceanicola granulosus. Strain M-M22(T) exhibited the highest 16S rRNA gene sequence similarity values (each 95.4 %) to the type strains of Oceanicola batsensis and Oceanicola nitratireducens and 93.9-94.8 % sequence similarity to the type strains of the other Oceanicola species Strain M-M22(T) was found to contain Q-10 as the predominant ubiquinone and C18:1 ω7c as the predominant fatty acid. Major polar lipids were determined to be phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid. The DNA G+C content was determined to be 67.6 mol%. Differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain M-M22(T) is distinguished from other Oceanicola species. On the basis of the data presented, strain M-M22(T) is considered to represent a novel species of the genus Oceanicola, for which the name Oceanicola litoreus sp. nov. is proposed. The type strain is M-M22(T) (= KCTC 32083(T) = CCUG 62794(T)).
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.forename: Mi-Hwa
authors.initials: MH
authors.lastname: Lee
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D000332:Aerobiosis; D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000693:Anaerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D007723:Korea; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D014451:Ubiquinone / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D012965:Sodium Chloride