title: Proteome coverage of the model plant Arabidopsis thaliana: implications for shotgun proteomic studies.
abstract: The recent aggregation of matched proteomics data for the model plant Arabidopsis has enabled the assessment of a diverse array of large scale shotgun proteomics data. A collection of over nine million matched peptides was used to assess proteome coverage and experimental parameters when compared to the theoretical tryptic peptide population. The analysis indicated that the experimentally identified median peptide mass was significantly higher than the theoretical median tryptic peptide in Arabidopsis. This finding led to a critical examination of precursor scan ranges currently being employed by shotgun proteomic studies. The analysis revealed diminishing returns at the high end scan range and opportunities for greater coverage and identifications at the low mass range. Based on these findings, a recommended basic scan range of 300 to 1200m/z would suitably capture the peptide population in shotgun proteomic analyses in Arabidopsis.
authors.affiliation: Joint BioEnergy Institute and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
authors.forename: Gregory W
authors.initials: GW
authors.lastname: Mann
authors.forename: Hiren J
authors.initials: HJ
authors.lastname: Joshi
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Petzold
authors.forename: Joshua L
authors.initials: JL
authors.lastname: Heazlewood
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000235:genetics; D040901:Proteomics / Q000379:methods* / Q000592:standards; D053719:Tandem Mass Spectrometry; D014357:Trypsin / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D020543:Proteome; D014357:Trypsin