title: Prenatal maternal restraint stress exposure alters the reproductive hormone profile and testis development of the rat male offspring.
abstract: Several studies have demonstrated that the presence of stressors during pregnancy induces adverse effects on the neuroendocrine system of the offspring later in life. In the present work, we investigated the effects of early programming on the male reproductive system, employing a prenatal stress (PS) paradigm. This study found that when pregnant dams were placed in a plastic restrainer three times a day during the last week of pregnancy, the offspring showed reduced anogenital distance and delayed testicular descent. Serum luteinising hormone (LH) and follicle-stimulating hormone (FSH) levels were decreased at postnatal day (PND) 28 and testosterone was decreased at PND 75. Increased testosterone plus dihydrotestosterone (T + DHT) concentrations correlated with increased testicular 5α Reductase-1 (5αR-1) mRNA expression at PND 28. Moreover, PS accelerated spermatogenesis at PND 35 and 60, and increased mean seminiferous tubule diameter in pubertal offspring and reduced Leydig cell number was observed at PND 35 and 60. PS offspring had increased androgen receptor (AR) mRNA level at PND 28, and at PND 35 had increased the numbers of Sertoli cells immunopositive for AR. Overall, the results confirm that stress during gestation can induce long-term effects on the male offspring reproductive system. Of particular interest is the pre-pubertal imbalance of circulating hormones that probably trigger accelerated testicular development, followed by an increase in total androgens and a decrease in testosterone concentration during adulthood. Exposure to an unfavourable intrauterine environment might prepare for harsh external conditions by triggering early puberty, increasing reproductive potential.
authors.affiliation: IQUIFIB, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina .
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: Pallarés
authors.forename: Ezequiela
authors.initials: E
authors.lastname: Adrover
authors.forename: Carlos Javier
authors.initials: CJ
authors.lastname: Baier
authors.forename: Nadia S
authors.initials: NS
authors.lastname: Bourguignon
authors.forename: Melisa C
authors.initials: MC
authors.lastname: Monteleone
authors.forename: Marcela A
authors.initials: MA
authors.lastname: Brocco
authors.forename: Silvia I
authors.initials: SI
authors.lastname: González-Calvar
authors.forename: Marta C
authors.initials: MC
authors.lastname: Antonelli
mesh_terms: D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000096:biosynthesis; D000818:Animals; D013196:Dihydrotestosterone / Q000097:blood; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D007985:Leydig Cells / Q000378:metabolism; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D018811:Maternal Exposure; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011944:Receptors, Androgen / Q000378:metabolism; D012149:Restraint, Physical; D013091:Spermatogenesis; D013315:Stress, Psychological; D013737:Testis / Q000254:growth & development*; D013739:Testosterone / Q000097:blood
chemical_list: D012333:RNA, Messenger; D011944:Receptors, Androgen; D013196:Dihydrotestosterone; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase