title: [Molecular cloning, sequence analysis and stress-related changes of the heat shock protein 60 gene in Neobenedenia melleni].
abstract: Heat shock protein 60 is an essential chaperone that can maintain the natural structure and function of mitochondrial proteins. Here, we successfully cloned the full length cDNA of HSP60 from Neobenedenia melleni, designated as NmHSP60. Real-time quantitative PCR and Western blot were used to analyze the expression change of NmHSP60 under different temperature and salinity. Compared with the typical 25 Degrees Celsius, expressions decreased dramatically in eggs and adults at 18 Degrees Celsius. Conversely, at 32 Degrees Celsius, expression increase dramatically in adults. Compared with salinity 24, expressions were significantly down-regulated in adults at salinity 18, and up-regulated in eggs at salinity 30. Experimental results suggest that NmHSP60 may play an significant role in N. melleni's adaptation to adverse environmental conditions.
authors.affiliation: Ningbo University, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Jiong
authors.initials: J
authors.lastname: Chen
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Shi
authors.forename: Xin-Jiang
authors.initials: XJ
authors.lastname: Lu
authors.forename: Ming-Yun
authors.initials: MY
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018834:Chaperonin 60 / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D005260:Female; D015801:Helminth Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010063:Ovum / Q000254:growth & development / Q000378:metabolism; D010802:Phylogeny; D010986:Platyhelminths / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D017421:Sequence Analysis
chemical_list: D018834:Chaperonin 60; D015801:Helminth Proteins