title: Scl gene construction, expression and effect on hemangioma.
abstract: Hemangioma is a tumor that causes vascular endothelial cell hyperplasia, which commonly occur in newborns. Angiogenesis inhibitor targets the processes of angiogenesis, including the proliferation of vascular endothelial cells. A DNA sequence named Scl was designed, recombined into Pichia Pastoris, expressed by fermenting the engineered strain in a bioreactor, and purified the recombinant Scl by SP-sepharose fast flow. Scl can inhibit CAM angiogenesis. Only 1 μg of Scl significantly suppressed the growth of CAM blood vessel, similar to that of 25 μg of angiostatin. Scl showed a strong cytotoxicity on hemangioma cell (ATCC CRL No. 2587). After the drug acted for 24 h, the OD 570 measured value of the PBS control group averaged 1.873, whereas that of the Sc1 drug group was 0.692 (P < 0.01). Using the DeadEndTM Fluorometric TUNEL System, the detection results showed that 92 % of hemangioma cell apoptosis was observed in the Scl protein group, but only 1.3 % in the PBS control group (P < 0.01). After 2 weeks of treatment with the hemangioma model (cock's wattle) of the PBS group, 151 blood vessels with 100 views (40×) were obtained, whereas 250 in the PBS group (P < 0.01). During the two-week medication, the hemangioma model of the PBS group increased by 1.18 cm, whereas only 0.58 cm in the Scl drug group (P < 0.01).
authors.affiliation: Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Biology and Genetic Resources of Torpical Crops, Ministry of Agriculture, Haikou 571101, Hainan, China.
authors.forename: H X
authors.initials: HX
authors.lastname: Yin
authors.forename: Z H
authors.initials: ZH
authors.lastname: Zhang
authors.forename: J C
authors.initials: JC
authors.lastname: Shen
authors.forename: A L
authors.initials: AL
authors.lastname: Zhang
authors.forename: T Y
authors.initials: TY
authors.lastname: Zhang
authors.forename: J X
authors.initials: JX
authors.lastname: Luo
authors.forename: C Y
authors.initials: CY
authors.lastname: Fu
authors.forename: X P
authors.initials: XP
authors.lastname: Yi
authors.forename: Z W
authors.initials: ZW
authors.lastname: Liu
authors.forename: S S
authors.initials: SS
authors.lastname: Yang
authors.forename: B
authors.initials: B
authors.lastname: Zhou
authors.forename: X
authors.initials: X
authors.lastname: Fu
mesh_terms: D020533:Angiogenesis Inhibitors / Q000008:administration & dosage; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001483:Base Sequence; D045744:Cell Line, Tumor; D002645:Chickens / Q000235:genetics / Q000254:growth & development; D042783:Endothelial Cells / Q000187:drug effects* / Q000473:pathology; D006391:Hemangioma / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D006801:Humans; D006965:Hyperplasia / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D008969:Molecular Sequence Data; D009389:Neovascularization, Pathologic / Q000188:drug therapy* / Q000235:genetics; D010455:Peptides / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000235:genetics; D010843:Pichia
chemical_list: D020533:Angiogenesis Inhibitors; D010455:Peptides