title: Modulation of hsa-miR-26b levels following adipokine stimulation.
abstract: MicroRNAs (miRNAs) are short non-coding RNAs that are involved in post-transcriptional regulation of gene expression. Hsa-miR-26b is an intronic miRNA located in the intron of CTDSP1 (carboxy-terminal domain, RNA polymerase II, polypeptide A, small phosphatase 1). In the present study, the expression of hsa-miR-26b was examined during human preadipocyte differentiation. 15 days after induction of differentiation, mature human adipocytes were treated with adipokines. Hsa-miR-26b was differentially expressed during human preadipocyte differentiation. Tumor necrosis factor-α (TNF-α), leptin and resistin, but not interleukin-6, caused downregulation of hsa-miR-26b expression in adipocytes. These results suggest that the expression of hsa-miR-26b is affected by TNF-α, leptin and resistin and that hsa-miR-26b may be an important mediator in regulating the obesity-related insulin sensitivity and inflammatory responses.
authors.affiliation: Department of Laboratory Medicine, The 82nd Hospital of the People's Liberation Army, No.100 Jiankang East Road, Huaian 223001, China.
authors.forename: Guangfeng
authors.initials: G
authors.lastname: Xu
authors.forename: Chenbo
authors.initials: C
authors.lastname: Ji
authors.forename: Chunmei
authors.initials: C
authors.lastname: Shi
authors.forename: Hailong
authors.initials: H
authors.lastname: Fu
authors.forename: Lingling
authors.initials: L
authors.lastname: Zhu
authors.forename: Lu
authors.initials: L
authors.lastname: Zhu
authors.forename: Lulian
authors.initials: L
authors.lastname: Xu
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Yueying
authors.initials: Y
authors.lastname: Feng
authors.forename: Yaping
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xirong
authors.initials: X
authors.lastname: Guo
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D054392:Adipokines / Q000494:pharmacology*; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology; D020738:Leptin / Q000494:pharmacology; D035683:MicroRNAs / Q000235:genetics*; D009765:Obesity / Q000235:genetics / Q000378:metabolism; D052243:Resistin / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D054392:Adipokines; D015850:Interleukin-6; D020738:Leptin; D035683:MicroRNAs; D052243:Resistin; D014409:Tumor Necrosis Factor-alpha; C528163:MIRN26 microRNA, human