title: Leptin and queen ovary: new insights about ovulation.
abstract: Leptin has been proven to be crucial in the ovulatory process. Aims of this study are to assess the expression of leptin receptor (Ob-R) in the ovaries of queens at estrus and to evaluate the capability of leptin in modulating ovarian contractility in vitro. Right ovaries underwent immunoblot analysis. Left ovaries were mounted in an organ bath under physiological condition and exposed to murine leptin (10(-6) M). Immunoblot analysis showed that the queen ovary expresses leptin receptor at estrus. Leptin at the dose of 10(-6) M significantly reduced the contractile activity of the ovary. The presence of ovarian Ob-R and leptin inhibitory effects on ovarian contractility suggest leptin implication in the modulation of ovarian activity, as well as in ovulatory disorders.
authors.affiliation: Department of Emergency and Organs Transplantations, University of Bari Aldo Moro, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Trisolini
authors.forename: M
authors.initials: M
authors.lastname: Albrizio
authors.forename: M T
authors.initials: MT
authors.lastname: Roscino
authors.forename: M
authors.initials: M
authors.lastname: Pantaleo
authors.forename: A
authors.initials: A
authors.lastname: Rizzo
authors.forename: R L
authors.initials: RL
authors.lastname: Sciorsci
mesh_terms: D000818:Animals; D002415:Cats; D004305:Dose-Response Relationship, Drug; D004971:Estrus / Q000378:metabolism / Q000502:physiology; D005260:Female; D015151:Immunoblotting / Q000662:veterinary; D020738:Leptin / Q000494:pharmacology / Q000502:physiology*; D051379:Mice; D010053:Ovary / Q000187:drug effects / Q000502:physiology*; D010060:Ovulation / Q000187:drug effects / Q000502:physiology*; D054411:Receptors, Leptin / Q000502:physiology
chemical_list: D020738:Leptin; D054411:Receptors, Leptin