title: Cytotoxic and genotoxic potential of tannery waste contaminated soils.
abstract: Soil samples from agricultural fields in the vicinity of industrial area of Jajmau, Kanpur (India) were collected and found to be heavily contaminated with various toxic heavy metals. GC-MS analysis revealed the presence of organic compounds mainly phthalates in contaminated soils. Samples were extracted using dichloromethane (DCM) and hexane solvents, and the extracts were assayed for genotoxic potential using three different bioassays namely Ames Salmonella/mammalian microsome test, DNA repair defective Escherichia coli K-12 mutants and Allium cepa chromosomal aberration assay. TA98 was found to be the most sensitive strain to all the soil extracts tested. The highest mutagenic potential was observed in DCM extracts of soil as compared with hexane extracts for each strain of Salmonella typhimurium. DCM extracts of the soil exhibited maximum damage to the cells at a dose of 40 μl of soil extracts/ml of culture after a 6-h treatment. The survival was 23% in polA, 40% in lexA and 53% in recA mutants when treated with DCM extract of site I. In A. cepa assay, all the test concentrations of soil extracts (5-100%) affected mitotic index in a dose-dependent manner and several types of abnormalities were observed at different mitotic stages with the treatments: C-mitosis, anaphase bridges, laggards, binucleated cells, stickiness, broken and unequal distributions of chromosomes at anaphase stage of cell division. The soil is accumulating a large number of pollutants as a result of wastewater irrigation and this practice of accumulation has an adverse impact on soil health.
authors.affiliation: Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh-202002, India.
authors.forename: Farhana
authors.initials: F
authors.lastname: Masood
authors.forename: Abdul
authors.initials: A
authors.lastname: Malik
mesh_terms: D002869:Chromosome Aberrations; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D048168:Escherichia coli K12 / Q000187:drug effects / Q000235:genetics; D006586:Hexanes / Q000737:chemistry; D007194:India; D007220:Industrial Waste; D019216:Metals, Heavy / Q000032:analysis / Q000633:toxicity*; D008752:Methylene Chloride / Q000737:chemistry; D008940:Mitotic Index; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000633:toxicity; D019697:Onions / Q000187:drug effects / Q000235:genetics; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D012989:Soil Pollutants / Q000008:administration & dosage / Q000032:analysis / Q000633:toxicity*; D013633:Tanning
chemical_list: D006586:Hexanes; D007220:Industrial Waste; D019216:Metals, Heavy; D009153:Mutagens; D012989:Soil Pollutants; D008752:Methylene Chloride