title: Genetic variation of North American Triatomines (Insecta: Hemiptera: Reduviidae): initial divergence between species and populations of Chagas disease vector.
abstract: The triatomines vectors of Trypanosoma cruzi are principal factors in acquiring Chagas disease. For this reason, increased knowledge of domestic transmission of T. cruzi and control of its insect vectors is necessary. To contribute to genetic knowledge of North America Triatominae species, we studied genetic variations and conducted phylogenetic analysis of different triatomines species of epidemiologic importance. Our analysis showed high genetic variations between different geographic populations of Triatoma mexicana, Meccus longipennis, M. mazzottii, M. picturatus, and T. dimidiata species, suggested initial divergence, hybridation, or classifications problems. In contrast, T. gerstaeckeri, T. bolivari, and M. pallidipennis populations showed few genetics variations. Analysis using cytochrome B and internal transcribed spacer 2 gene sequences indicated that T. bolivari is closely related to the Rubrofasciata complex and not to T. dimidiata. Triatoma brailovskyi and T. gerstaeckeri showed a close relationship with Dimidiata and Phyllosoma complexes.
authors.affiliation: Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Distrito Federal, México. besgu@biomedicas.unam.mx
authors.forename: Bertha
authors.initials: B
authors.lastname: Espinoza
authors.forename: Jose Alejandro
authors.initials: JA
authors.lastname: Martínez-Ibarra
authors.forename: Guiehdani
authors.initials: G
authors.lastname: Villalobos
authors.forename: Patricia
authors.initials: P
authors.lastname: De La Torre
authors.forename: Juan Pedro
authors.initials: JP
authors.lastname: Laclette
authors.forename: Fernando
authors.initials: F
authors.lastname: Martínez-Hernández
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000635:transmission; D003001:Cloning, Molecular; D045303:Cytochromes b / Q000235:genetics; D014644:Genetic Variation; D007303:Insect Vectors / Q000145:classification* / Q000235:genetics / Q000469:parasitology; D018895:Microsatellite Repeats; D009656:North America; D010802:Phylogeny; D058974:Phylogeography; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014224:Triatoma / Q000145:classification* / Q000235:genetics*
chemical_list: D045303:Cytochromes b