title: [The application of isoprenaline provocation test on rat model of alcoholic cardiomyopathy].
abstract: OBJECTIVE
  To study a simple and reliable method to produce the rat model of alcoholic cardiomyopathy, evaluating the model with isoprenaline provocation test (IPT) and morphological indicators.
  
  
  METHODS
  Adult male rats were intragastrically infused of wine (52% v/v) at 20 g/(kg x d) for 15 days with the general condition, the change of eating amount and weight being observed. The levels of mean left ventricle systolic pressure (mLVSP), mean left ventricle diastolic pressure (mLVDP), mean left ventricle pressure (mLVP), heart ratio (HR), maximal rise velocity of left ventricular pressure (+ dp/dtmax), maximal fall velocity of left ventricular pressure (- dp/dtmax) and the reaction of isoprenaline were examined by left ventricular cannulation, and the morphological change was observed with optical microscope and transmission electron microscopy.
  
  
  RESULTS
  Both diastolic and systolic function of the model rats was lower than that of the control group as well as the cardiac energy reserve induced by IPT. Pathological observation demonstrated myocardial hypertrophy, myocardiocyte necrosis and infiltration of inflammatory cells. The transmission electron microscopy showed that mitochondria became enlarged or crimpled with fused or disappeared cristae and myofibrils dissolved and fractured.
  
  
  CONCLUSIONS
  The adult male SD model rats exhibit diabetic cardiomyopathy by intragastric infusion of wine (52% v/v) at 20 g/(kg x d) for 15 days. IPT can induce the cardiac energy reserve and evaluate that accurately, displaying the hidden heart dysfunction.
authors.affiliation: Department of Pathology, First Clinical Medical College of Fujian Medical University, Fuzhou 350004, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zou
authors.forename: Song-Bai
authors.initials: SB
authors.lastname: Xu
authors.forename: Qiu-Juan
authors.initials: QJ
authors.lastname: Fang
authors.forename: Rui-Xing
authors.initials: RX
authors.lastname: Wang
mesh_terms: D000818:Animals; D002310:Cardiomyopathy, Alcoholic / Q000503:physiopathology*; D004195:Disease Models, Animal; D007545:Isoproterenol; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007545:Isoproterenol