title: Synaptic vesicle protein 2 (SV2) isoforms.
abstract: New molecular markers of cancer had emerged with novel applications in cancer prevention and therapeutics, including for breast cancer of unknown causes, which has a high impact on the health of women worldwide. The purpose of this research was to determine protein and mRNA expression of synaptic vesicle 2 (SV2) isoforms A, B and C in breast cancer cell lines. Cultured cell lines MDA-MB-231, SKBR3, T47D were lysed and their protein and mRNA expression analyzed by real-time PCR and western blot technique, respectively. SV2A, B proteins were identified in non-tumor (MCF-10A) and tumor cell lines (MDA-MB-231 and T47D) while SV2C only was found in the T47D cell line. Furthermore, the genomic expression was consistent with protein expression for a such cell line, but in MDA-MB-231 there was no SV2B genomic expression, and the SV2C mRNA and protein were not found in the non tumoral cell line. These findings suggest a possible cellular transdifferentiation to neural character in breast cancer, of possible relevance to cancer development, and point to possible use of SV2 as molecular marker and a vehicle for cancer treatment with botulinum toxin.
authors.affiliation: Research Support Group, National Institute of Rehabilitation, Mexico.
authors.forename: Cindy
authors.initials: C
authors.lastname: Bandala
authors.forename: A
authors.initials: A
authors.lastname: Miliar-García
authors.forename: C M
authors.initials: CM
authors.lastname: Mejía-Barradas
authors.forename: M
authors.initials: M
authors.lastname: Anaya-Ruiz
authors.forename: J P
authors.initials: JP
authors.lastname: Luna-Arias
authors.forename: C I
authors.initials: CI
authors.lastname: Bazán-Méndez
authors.forename: M
authors.initials: M
authors.lastname: Gómez-López
authors.forename: S
authors.initials: S
authors.lastname: Juárez-Méndez
authors.forename: E
authors.initials: E
authors.lastname: Lara-Padilla
mesh_terms: D015153:Blotting, Western; D001940:Breast / Q000378:metabolism*; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; C076414:SV2A protein, human