title: Pseudomonas aeruginosa infections in patients, hospital means, and personnel's specimens.
abstract: BACKGROUND
  Pseudomonas aeruginosa is a common cause of nosocomial infections. It exhibits innate resistance to a wide range of antibiotics. This study was performed to determine clonal characteristic of P. aeruginosa isolated from clinical specimens, hospital means, and hospital personnel by PCR- ribotyping patterns.
  
  
  METHODS
  A total of 104 P. aeruginosa were isolated from clinical and environmental samples (59 clinical, 45 hospital means and hospital personnel). P. aeruginosa was identified by standard bacteriological methods, mucoid colony morphotypes, and antibiotic resistance rate. The genomes of isolates were extracted and all considered species were confirmed by 16S rDNA- based PCR assay. Then all isolates were genotyped by the 16S - 23SrDNA and Hinf1 restriction enzyme technique.
  
  
  RESULTS
  Antibacterial sensitivity pattern of isolates showed clinical and environmental specimens were approximately identical (high antibiotic resistance to Ceftazidime and low antibiotic resistance to Amikacin). Colony morphotypes of specimens revealed that mucoid type of clinical isolates were more than that of environmental isolates. Among clinical and environmental strains P1; (570 bp) was the most prevalence pattern.
  
  
  CONCLUSIONS
  Antibiotic resistance, phenotypic characterization, and PCR- ribotyping pattern showed there is clonal relatedness between clinical and environmental isolates and environment could be a main reservoir for P. aeruginosa infections in hospital.
authors.affiliation: Department of Bacteriology and Virology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Fazeli
authors.forename: Reza
authors.initials: R
authors.lastname: Akbari
authors.forename: Sharareh
authors.initials: S
authors.lastname: Moghim
authors.forename: Tahmineh
authors.initials: T
authors.lastname: Narimani
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Arabestani
authors.forename: Ali Reza
authors.initials: AR
authors.lastname: Ghoddousi