title: Dehydrin2 is a stress-inducible, whereas Dehydrin1 is constitutively expressed but up-regulated gene under varied cues in tea [Camellia sinensis (L.) O. Kuntze].
abstract: Two expressed sequence tags with similarity to dehydrin were cloned to full-length (CsDHN1 and CsDHN2) through rapid amplification of cDNA ends. CsDHN1 and CsDHN2 were 1,027 and 992 base pair long and encoded for predicted polypeptides of 260 and 201 amino acids, respectively. Deduced CsDHN1 protein had a S-segment and three lysine-rich consensus motifs (K-segments). The Y-segment was, however, absent in the deduced CsDHN1. CsDHN2 had three Y motifs, one S-segment and two K-segments. Expression of CsDHN1 was visible at all the time points of study, though up-regulation was observed in response to winter dormancy (WD) as well as abiotic stresses [low temperature, sodium chloride, polyethylene glycol, and hydrogen peroxide]. Expression of CsDHN2 was strongly up-regulated within 1 h of exposure to abiotic stresses as well in the tissue harvested during WD in contrast to the respective "control" for abiotic stresses and in tissue during the period of active growth, when the expression was not detectable.
authors.affiliation: Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, P.O. Box No. 6, Palampur 176061, Himachal Pradesh, India.
authors.forename: Asosii
authors.initials: A
authors.lastname: Paul
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kumar
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D028241:Camellia sinensis / Q000235:genetics* / Q000254:growth & development; D003463:Cues; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D011092:Polyethylene Glycols / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins; C070556:dehydrin proteins, plant; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D006861:Hydrogen Peroxide