title: Multiplexed volumetric bar-chart chip for point-of-care diagnostics.
abstract: Microfluidics have become an enabling technology for point-of-care and personalized diagnostics. Desirable capabilities of microfluidics-based diagnostic devices include simplicity, portability, low cost and the performance of multiplexed and quantitative measurements, ideally in a high-throughput format. Here we present the multiplexed volumetric bar-chart chip (V-Chip), which integrates all these capabilities in one device. A key feature of the V-Chip is that quantitative results are displayed as bar charts directly on the device-without the need for optical instruments or any data processing or plotting steps. This is achieved by directly linking oxygen production by catalase, which is proportional to the concentration of the analyte, with the displacement of ink along channels on the device. We demonstrate the rapid quantification of protein biomarkers in diverse clinical samples with the V-Chip. The development of the V-Chip thus opens up the possibility of greatly simplified point-of-care and personalized diagnostics.
authors.affiliation: Department of Nanomedicine, The Methodist Hospital Research Institute, Houston, Texas 77030, USA.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Song
authors.forename: Yuanqing
authors.initials: Y
authors.lastname: Zhang
authors.forename: Paul E
authors.initials: PE
authors.lastname: Bernard
authors.forename: James M
authors.initials: JM
authors.lastname: Reuben
authors.forename: Naoto T
authors.initials: NT
authors.lastname: Ueno
authors.forename: Ralph B
authors.initials: RB
authors.lastname: Arlinghaus
authors.forename: Youli
authors.initials: Y
authors.lastname: Zu
authors.forename: Lidong
authors.initials: L
authors.lastname: Qin
mesh_terms: D015415:Biomarkers / Q000097:blood; D002374:Catalase / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods; D019095:Point-of-Care Systems; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers; D006861:Hydrogen Peroxide; D002374:Catalase