title: Efficient saccharification for non-treated cassava pulp by supplementation of Clostridium thermocellum cellulosome and Thermoanaerobacter brockii β-glucosidase.
abstract: Cassava pulp containing 60% starch and 20% cellulose is a promising renewable source for bioethanol. The starch granule was observed to tightly bind cellulose fiber. To achieve an efficient degradation for cassava pulp, saccharification tests without pre-gelatinization treatment were carried out using combination of commercial α-amylase with cellulosome from Clostridium thermocellum S14 and β-glucosidase (rCglT) from Thermoanaerobacter brockii. The saccharification rate for cassava pulp was shown 59% of dry matter. To obtain maximum saccharification rate, glucoamylase (GA) from C. thermocellum S14 was supplemented to the combination. The result showed gradual increase in the saccharification rate to 74% (dry matter). Supplementation of GA to the combination of commercial α-amylase, cellulosome and rCglT is powerful method for efficient saccharification of cassava pulp without pretreatment.
authors.affiliation: Nanotechnology and Biotechnology Division, Kasetsart Agricultural and Agro-Industrial Product Improvement Institute, Kasetsart University, 50, Ngamwongwan Rd., Bangkok 10900, Thailand. p_vaithanomsat@yahoo.com
authors.forename: Pilanee
authors.initials: P
authors.lastname: Vaithanomsat
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kosugi
authors.forename: Waraporn
authors.initials: W
authors.lastname: Apiwatanapiwat
authors.forename: Warunee
authors.initials: W
authors.lastname: Thanapase
authors.forename: Rattiya
authors.initials: R
authors.lastname: Waeonukul
authors.forename: Chakrit
authors.initials: C
authors.lastname: Tachaapaikoon
authors.forename: Patthra
authors.initials: P
authors.lastname: Pason
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Mori
mesh_terms: D056804:Biofuels; D001709:Biotechnology / Q000379:methods; D002241:Carbohydrates / Q000096:biosynthesis*; D044603:Cellulosomes / Q000201:enzymology* / Q000235:genetics; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D048013:Acetivibrio thermocellus / Q000201:enzymology*; D017931:DNA Primers / Q000235:genetics; D005087:Glucan 1,4-alpha-Glucosidase / Q000235:genetics / Q000378:metabolism; D002365:Manihot / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics; D046968:Thermoanaerobacter / Q000201:enzymology*; D000516:alpha-Amylases / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D056804:Biofuels; D002241:Carbohydrates; D017931:DNA Primers; D011994:Recombinant Proteins; D000516:alpha-Amylases; D001617:beta-Glucosidase; D005087:Glucan 1,4-alpha-Glucosidase