title: Evaluation of biological activities of a groundnut (Apios americana Medik) extract containing a novel isoflavone.
abstract: Groundnut (Apios americana Medik) contains a novel isoflavone, genistein-7-O-gentiobioside. In the present study, we examined the biological activities of an alcohol extract of groundnut containing genistein-7-O-gentiobioside as the main component. Although the groundnut extract by itself did not show antioxidative activity, it drove the antioxidative system in cells. Pretreatment of human breast carcinoma MCF-7 cells for 24 h with the groundnut extract and soybean isoflavone increased gene expression of heme oxygenase-1 (HO-1), a major antioxidative stress enzyme. These groundnut extract-treated cells showed antioxidative activity against free radicals derived from a radical initiator. Pretreatment of cells with 100 μg/mL groundnut extract prevented the depletion of glutathione by the radical initiator; however, treatment with 100 μg/mL of soybean isoflavone injured the cell membrane, indicating that glutathione might be released to the extracellular environment. These results suggest that the groundnut extract had isoflavone-like activity. Like soybean, groundnuts are a good source of isoflavones.
authors.affiliation: Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan.
authors.forename: Mizuki
authors.initials: M
authors.lastname: Takashima
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nara
authors.forename: Etsuo
authors.initials: E
authors.lastname: Niki
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Hagihara
authors.forename: Mayumi
authors.initials: M
authors.lastname: Stowe
authors.forename: Masanori
authors.initials: M
authors.lastname: Horie
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D007887:Fabaceae / Q000737:chemistry*; D005260:Female; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D007529:Isoflavones; D010936:Plant Extracts