title: Comparison of CCD-equipped laser speckle flowgraphy with hydrogen gas clearance method in the measurement of optic nerve head microcirculation in rabbits.
abstract: The aim of this study was to verify the correlation between mean blur rate (MBR) obtained with CCD-equipped laser speckle flowgraphy (LSFG) and capillary blood flow (CBF) obtained by the hydrogen gas clearance method in rabbit optic nerve head (ONH). Using Japanese white rabbits under systemic anesthesia, a hydrogen electrode was inserted an area of the ONH free from superficial capillaries. MBR was measured with LSFG near the hydrogen electrode. CBF and MBR were measured in the range of 32.4-83.5 mL/min/100 g and 3.5-6.0, respectively. MBR and CBF were significantly correlated (r = 0.73, P < 0.01, n = 14). After inhalation of carbon dioxide (CO(2)) or intravenous administration of endothelin-1 (ET-1), MBR and CBF were changed in the relative range of 0.74-1.27 and 0.76-1.35, respectively. The relative changes in MBR and CBF induced by CO(2) and ET-1 were also significantly correlated (r = 0.67, P < 0.01). The current results suggest that MBR may correlate with CBF and also change with CBF, as an index of blood flow in the ONH, linearly.
authors.affiliation: Research Laboratory for Drug Development, Senju Pharmaceutical Co., Ltd., 1-5-4 Murotani, Nishi-ku, Kobe, Hyogo 651-2241, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Sugiyama
authors.forename: Hideki
authors.initials: H
authors.lastname: Tokushige
authors.forename: Takatoshi
authors.initials: T
authors.lastname: Maeno
authors.forename: Toru
authors.initials: T
authors.lastname: Nakazawa
authors.forename: Tsunehiko
authors.initials: T
authors.lastname: Ikeda
authors.forename: Makoto
authors.initials: M
authors.lastname: Araie
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D001783:Blood Flow Velocity; D002196:Capillaries / Q000187:drug effects / Q000502:physiology*; D002245:Carbon Dioxide / Q000008:administration & dosage; D019332:Endothelin-1 / Q000008:administration & dosage; D005740:Gases; D006207:Half-Life; D006859:Hydrogen / Q000008:administration & dosage / Q000493:pharmacokinetics; D007275:Injections, Intravenous; D017736:Ion-Selective Electrodes; D017078:Laser-Doppler Flowmetry / Q000295:instrumentation; D016014:Linear Models; D008297:Male; D008833:Microcirculation / Q000187:drug effects; D009900:Optic Nerve / Q000098:blood supply*; D011817:Rabbits; D012039:Regional Blood Flow; D013997:Time Factors
chemical_list: D019332:Endothelin-1; D005740:Gases; D002245:Carbon Dioxide; D006859:Hydrogen