title: Identification of anaerobic bacteria by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry with on-plate formic acid preparation.
abstract: Identification of anaerobic bacteria using phenotypic methods is often time-consuming; methods such as 16S rRNA gene sequencing are costly and may not be readily available. We evaluated 253 clinical isolates of anaerobic bacteria using the Bruker MALDI Biotyper (Bruker Daltonics, Billerica, MA) matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) system with a user-supplemented database and an on-plate formic acid-based preparation method and compared results to those of conventional identification using biochemical testing or 16S rRNA gene sequencing. A total of 179 (70.8%) and 232 (91.7%) isolates were correctly identified to the species and genus levels, respectively, using manufacturer-recommended score cutoffs. MALDI-TOF MS offers a rapid, inexpensive method for identification of anaerobic bacteria.
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: Bryan H
authors.initials: BH
authors.lastname: Schmitt
authors.forename: Scott A
authors.initials: SA
authors.lastname: Cunningham
authors.forename: Aaron L
authors.initials: AL
authors.lastname: Dailey
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Gustafson
authors.forename: Robin
authors.initials: R
authors.lastname: Patel
mesh_terms: D001421:Bacteria, Anaerobic / Q000737:chemistry* / Q000145:classification*; D001431:Bacteriological Techniques / Q000191:economics / Q000379:methods*; D005561:Formates / Q000378:metabolism; D013048:Specimen Handling / Q000191:economics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000191:economics / Q000379:methods*
chemical_list: D005561:Formates; C030544:formic acid