title: Lymphocyte recruitment via high endothelial venules in lymphoid stroma of Warthin's tumour.
abstract: Warthin's tumour is composed of bilayered oncocytic epithelium and organised lymphoid stroma, which resembles mucosa-associated lymphoid tissue (MALT); however, the histogenesis of the lymphoid stroma is not fully understood. We hypothesised that lymphocytes consisting of the stroma are recruited via high endothelial venules (HEVs) by the mechanism operating in normal lymphocyte homing in secondary lymphoid organs. The aim of this study was to determine immunohistochemically the molecules expressed on these HEVs.
  
  
  
  Tissue sections of Warthin's tumour (n = 10) were immunostained for vascular addressin-related antigens including peripheral lymph node addressin (PNAd) and mucosal addressin cell adhesion molecule 1 (MAdCAM-1). An L-selectin·IgM chimera in situ binding assay was also carried out. Triple immunostaining for PNAd, CD3, and CD20/CD79α was performed to determine which lymphocyte subsets are closely associated with these HEVs.
  
  
  
  HEVs in the lymphoid stroma of Warthin's tumour express PNAd, which is detected by MECA-79 as well as recently developed monoclonal antibodies S1 and S2. These HEVs were bound by L-selectin·IgM chimeras in a calcium-dependent manner, and numbers of lymphocytes, particularly T cells, attached to these HEVs.
  
  
  
  The lymphoid stroma of Warthin's tumour is most likely developed by lymphocytes recruited via HEVs.
authors.affiliation: Department of Molecular Pathology, Shinshu University Graduate School of Medicine, Matsumoto, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Ohya
authors.forename: Motohiro
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Sakai
authors.forename: Hiroto
authors.initials: H
authors.lastname: Kawashima
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Kageyama
authors.forename: Jun
authors.initials: J
authors.lastname: Nakayama
mesh_terms: D000235:Adenolymphoma / Q000378:metabolism / Q000473:pathology*; D015703:Antigens, CD / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism; D015815:Cell Adhesion Molecules; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D016131:Lymphocyte Subsets / Q000378:metabolism / Q000473:pathology; D008214:Lymphocytes / Q000378:metabolism / Q000473:pathology*; D008565:Membrane Proteins / Q000378:metabolism; D009088:Mucoproteins / Q000378:metabolism; D012468:Salivary Gland Neoplasms / Q000378:metabolism / Q000473:pathology*; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D014699:Venules / Q000378:metabolism / Q000473:pathology*
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; C069886:L-selectin counter-receptors; C504265:MADCAM1 protein, human; D008565:Membrane Proteins; D009088:Mucoproteins