title: Diagnostic implications of urinary liver-type fatty acid-binding protein and 8-hydroxy-2'-deoxyguanosine in forensic autopsy cases.
abstract: OBJECTIVE
  Liver-type fatty acid-binding protein (L-FABP) is a clinical biomarker of the progress of kidney disease. 8-Hydroxy-2'-deoxyguanosine (8-OHdG) is known as a biomarker of peroxidative DNA damage. We investigated both urinary L-FABP and 8-OHdG in forensic autopsy cases as biomarkers to elucidate the metabolic changes in survival periods after insults.
  
  
  METHODS
  In 196 urinary samples from forensic autopsy cases, we measured L-FABP and 8-OHdG by enzyme-linked immunosorbent assay (ELISA) and creatinine by enzymatic assay. Urinary L-FABP/Cr and 8-OHdG/Cr were obtained.
  
  
  RESULTS
  No significant correlation was observed between urinary L-FABP/Cr or 8-OHdG/Cr, and gender, age, or postmortem interval. Regarding urinary L-FABP/Cr or 8-OHdG/Cr, there were no significant differences among the causes of death. In the survival/agony period, urinary L-FABP/Cr under the cut-off value 31.3 might show that the survival/agony period was within 1 h. Under the cut-off value of urinary 8-OHdG/Cr, 17.8, might indicate that it is within 24 h.
  
  
  CONCLUSIONS
  Urinary L-FABP/Cr may rise within a relatively short survival/agony period, and urinary 8-OHdG/Cr may increase when the damage continues longer. Measuring the urinary L-FABP/Cr and 8-OHdG/Cr might be useful in elucidating the survival/agony period.
authors.affiliation: Department of Forensic Medicine, Faculty of Medicine, Fukuoka University, Japan. kasiwagi@fukuoka-u.ac.jp
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kashiwagi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Sugimura
authors.forename: Aya
authors.initials: A
authors.lastname: Matsusue
authors.forename: Kenji
authors.initials: K
authors.lastname: Hara
authors.forename: Brian
authors.initials: B
authors.lastname: Waters
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Kubo
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000652:urine; D002423:Cause of Death; D002648:Child; D002675:Child, Preschool; D003404:Creatinine / Q000652:urine; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay; D050556:Fatty Acid-Binding Proteins / Q000652:urine*; D005260:Female; D049429:Forensic Pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D013534:Survival; D013997:Time Factors; D014508:Urea / Q000652:urine; D055815:Young Adult
chemical_list: D015415:Biomarkers; C493319:FABP1 protein, human; D050556:Fatty Acid-Binding Proteins; D000080242:8-Hydroxy-2'-Deoxyguanosine; D014508:Urea; D003404:Creatinine; D003849:Deoxyguanosine