title: Agar barrier method for removal of yeast and other fungal contamination from Leishmania promastigotes culture.
abstract: The present study explains a novel method of Leishmania promastigotes culture decontamination. The method is based on motility of Leishmania promastigotes across agar barrier which facilitates decontamination from yeast and other non motile contamination. This is inexpensive,  easy, rapid and reliable physical method and is able to save valuable isolates in culture.
authors.affiliation: Rajendra Memorial Research Institute of Medical Sciences, Indian Council of Medical Research, Patna, Bihar, India.
authors.forename: Anil Kumar
authors.initials: AK
authors.lastname: Gupta
authors.forename: Shyam
authors.initials: S
authors.lastname: Narayan
authors.forename: Shubhankar Kumar
authors.initials: SK
authors.lastname: Singh
authors.forename: Ajit Kumar
authors.initials: AK
authors.lastname: Thakur
authors.forename: Pradeep
authors.initials: P
authors.lastname: Das
mesh_terms: D000362:Agar; D018929:Cell Culture Techniques / Q000379:methods; D002469:Cell Separation; D005658:Fungi / Q000302:isolation & purification*; D006801:Humans; D007891:Leishmania / Q000302:isolation & purification / Q000502:physiology*; D008124:Locomotion; D010274:Parasitology / Q000379:methods*
chemical_list: D000362:Agar