title: Multinozzle emitter array chips for small-volume proteomics.
abstract: High-throughput multiplexed proteomics of small-volume biospecimens will generate new opportunities in theranostics. Achieving parallel top-down and bottom-up mass spectrometry analyses of target proteins using a unified apparatus will improve proteome characterization. We have developed a novel silicon-based microfluidic device, multinozzle emitter array chip (MEA chip), as a new platform for small-volume proteomics using liquid chromatography-nanoelectrospray ionization mass spectrometry (LC-nanoESI-MS). We demonstrate parallel, on-chip, and online LC-MS analysis of hemoglobin and its tryptic digests directly from microliters of blood, achieving a detection limit of less than 5 red blood cells. Our MEA chip will enable clinical proteomics of small-volume samples.
authors.affiliation: Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
authors.forename: Pan
authors.initials: P
authors.lastname: Mao
authors.forename: Rafael
authors.initials: R
authors.lastname: Gomez-Sjoberg
authors.forename: Daojing
authors.initials: D
authors.lastname: Wang
mesh_terms: D002853:Chromatography, Liquid; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D040901:Proteomics; D012825:Silicon / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D006454:Hemoglobins; D012825:Silicon