title: One-step polymer surface modification for minimizing drug, protein, and DNA adsorption in microanalytical systems.
abstract: The non-specific adsorption of dissolved analytes strongly reduces the sensitivity and reliability in polymer microanalytical systems. Here, a one-step aqueous phase procedure modifies polymer material surfaces to strongly reduce their non-specific adsorption of a broad range of organic analytes including hydrophobic and hydrophilic drugs (0.23 < ClogP < 8.95), small and large proteins (insulin, albumin, IgG), and DNA. The coating is shown to limit the adsorption of even highly hydrophobic drugs (ClogP > 8) in their pharmaceutically relevant concentration range ≤100 nM. The low adsorption is mediated by photochemical conjugation, where polyethylene glycol (PEG) polymers in aqueous solution are covalently bound to the surface by UV illumination of dissolved benzophenone and a functionalized PEG. The method can coat the interior of polymer systems made from a range of materials commonly used in microanalytical systems, including polystyrene (PS), cyclic olefin copolymer (COC), liquid crystalline polymer (LCP), and polyimide (PI).
authors.affiliation: Department of Micro- and Nanotechnology, DTU Nanotech, Technical University of Denmark, Ørsteds Plads 345E, DK-2800 Kgs. Lyngby, Denmark.
authors.forename: Esben Kjær Unmack
authors.initials: EK
authors.lastname: Larsen
authors.forename: Niels B
authors.initials: NB
authors.lastname: Larsen
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry*; D007328:Insulin / Q000737:chemistry*; D013058:Mass Spectrometry; D015394:Molecular Structure; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013053:Spectrophotometry; D013499:Surface Properties; D014965:X-Rays
chemical_list: D007074:Immunoglobulin G; D007328:Insulin; D004364:Pharmaceutical Preparations; D011108:Polymers; D012710:Serum Albumin, Bovine; D004247:DNA