title: Identification of novel HLA-A 0201-restricted cytotoxic T lymphocyte epitopes from Zinc Transporter 8.
abstract: Numerous evidences demonstrated that type 1 diabetes (T1D) is due to a loss of immune tolerance to islet antigens, and CD8(+) T cells play an important role in the development of T1D. Zinc Transporter 8 (ZnT8) has emerged in recent years as a target of disease-associated autoreactive T cells in human T1D. However, ZnT8-associated CTL specific-peptides have not been identified. In this study, we predicted and identified HLA-A*0201-restricted cytotoxic T lymphocyte (CTL) epitopes derived from ZnT8, and utilized it to immunize HLA-A2.1/Kb transgenic (Tg) mice. The results demonstrated that peptides of ZnT8 containing residues 107-115, 115-123 and 145-153 could elicit specific CTLs in vitro, and induce diabetes in mice. The results suggest that these specific peptides are novel HLA-A*0201-restricted CTL epitopes, and could have therapeutic potential in preventing of T1D disease.
authors.affiliation: Endocrinology Division, Linyi People's Hospital, Linyi 276000, China. shufali@163.com
authors.forename: Shufa
authors.initials: S
authors.lastname: Li
authors.forename: Haiying
authors.initials: H
authors.lastname: Li
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.forename: Debin
authors.initials: D
authors.lastname: Lu
authors.forename: Wuquan
authors.initials: W
authors.lastname: Deng
authors.forename: Youzhao
authors.initials: Y
authors.lastname: Jiang
authors.forename: Zhongqi
authors.initials: Z
authors.lastname: Zhou
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D027682:Cation Transport Proteins / Q000276:immunology*; D002460:Cell Line; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology; D018604:Epitope Mapping; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D015789:HLA-A2 Antigen / Q000276:immunology*; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D000075703:Zinc Transporter 8
chemical_list: D027682:Cation Transport Proteins; D018984:Epitopes, T-Lymphocyte; C435939:HLA-A*02:01 antigen; D015789:HLA-A2 Antigen; D010455:Peptides; C492522:SLC30A8 protein, human; D000075703:Zinc Transporter 8