title: Variable domain antibodies specific for viral hemorrhagic septicemia virus (VHSV) selected from a randomized IgNAR phage display library.
abstract: Phage display libraries are used to screen for nucleotide sequences that encode immunoglobulin variable (V) regions that are specific for a target antigen. We previously constructed an immunoglobulin new antigen receptor (IgNAR) phage display library. Here we used this library to obtain an IgNAR V region that is specific for viral hemorrhagic septicemia virus (VHSV). A phage clone (clone 653) was found to be specific for VHSV by the biopanning method. The V region of clone 653 was used to construct a 6 × His tagged recombinant IgNAR-653 V protein (rIgNAR-653) using the Escherichia coli pET system. The rIgNAR-653 protein bound specifically to VHSV, confirming its activity.
authors.affiliation: Aquatic Biotechnology Center, College of Veterinary Medicine, Gyeongsang National University, Jinju, Gyeongnam, South Korea.
authors.forename: Maki
authors.initials: M
authors.lastname: Ohtani
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Hikima
authors.forename: Tae-Sung
authors.initials: TS
authors.lastname: Jung
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Kondo
authors.forename: Ikuo
authors.initials: I
authors.lastname: Hirono
authors.forename: Haruko
authors.initials: H
authors.lastname: Takeyama
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D061505:Cell Surface Display Techniques / Q000379:methods; D017931:DNA Primers / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli; D005432:Flounder; D007135:Immunoglobulin Variable Region / Q000276:immunology*; D008969:Molecular Sequence Data; D029141:Novirhabdovirus / Q000276:immunology*; D011946:Receptors, Antigen / Q000276:immunology*; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D017931:DNA Primers; D007135:Immunoglobulin Variable Region; D011946:Receptors, Antigen; D014764:Viral Proteins