title: Detection of foot-and-mouth disease serotype O by ELISA using a monoclonal antibody.
abstract: An ELISA assay with monoclonal antibody (MELISA) was used to type serotype O of foot-and-mouth disease virus (FMDV). All FMDV serotype O reference strains were positive by MELISA, while other viruses such as FMDV serotypes Asia 1, C, A and classical swine fever virus, swine vesicular disease virus, and porcine reproductive and respiratory syndrome virus remained negative. Further, FMDV serotype O positive samples were able to be detected by MELISA. This assay may be particularly suitable for diagnosis of FMDV serotype O infection in field stations.
authors.affiliation: College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu, P.R. China. haotaichen@163.com
authors.forename: Hao-tai
authors.initials: HT
authors.lastname: Chen
authors.forename: Yun-hua
authors.initials: YH
authors.lastname: Peng
authors.forename: Yong-guang
authors.initials: YG
authors.lastname: Zhang
authors.forename: Xiang-tao
authors.initials: XT
authors.lastname: Liu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000914:Antibodies, Viral / Q000737:chemistry; D002460:Cell Line; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D005536:Foot-and-Mouth Disease / Q000175:diagnosis* / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D012015:Reference Standards; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000821:virology
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral