title: Anti-CD30-targeted gold nanoparticles for photothermal therapy of L-428 Hodgkin's cell.
abstract: OBJECTIVE
  Due to the efficient bioconjugation and highly photothermal effect, gold nanoparticles can stain receptor-overexpressing cancer cells through specific targeting of ligands to receptors, strongly absorb specific light and efficiently convert it into heat based on the property of surface plasmon resonance, and then induce the localized protein denaturation and cell death.
  
  
  METHODS
  Two gold nanoparticle-antibody conjugates, gold-BerH2 antibody (anti-CD30 receptor) and gold-ACT1 antibody (anti-CD25-receptor), were synthesized. Gold-BerH2 conjugates can specifically bind to the surface of L-428 Hodgkin's cells, and gold-ACT1 conjugates were used for the control. The gold nanoparticle-induced L-428 cell-killing experiments were implemented with different experimental parameters.
  
  
  RESULTS
  At a relatively low concentration of gold and short incubation time, the influence of cytotoxicity of gold on cell viability can be overlooked. Under laser irradiation at suitable power, the high killing efficiency of gold-targeted L-428 cells was achieved, but little damage was done to nontargeted cancer cells.
  
  
  CONCLUSIONS
  Gold nanoparticle-mediated photothermal therapy provides a relatively safe therapeutic technique for cancer treatment.
authors.affiliation: Life Sciences Research Center, School of Life Sciences and Technology, Xidian University, Xi'an, Shaanxi, China. xiaochaoqu@gmail.com
authors.forename: Xiaochao
authors.initials: X
authors.lastname: Qu
authors.forename: Cuiping
authors.initials: C
authors.lastname: Yao
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.forename: Zhenxi
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D045744:Cell Line, Tumor; D006046:Gold / Q000627:therapeutic use*; D006689:Hodgkin Disease / Q000473:pathology / Q000628:therapy*; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D017730:Ki-1 Antigen / Q000276:immunology*; D053768:Metal Nanoparticles / Q000627:therapeutic use*; D010789:Phototherapy / Q000379:methods*; D016896:Treatment Outcome
chemical_list: D000911:Antibodies, Monoclonal; D017730:Ki-1 Antigen; D006046:Gold