title: The effect of agility exercise on eicosanoid excretion, oxidant status, and plasma lactate in dogs.
abstract: BACKGROUND
  The objective was to determine the effects of agility exercise on dogs of different skill levels with respect to urinary eicosanoids, urinary 15F2t-isoprostane (lipid peroxidation marker) and hematological/biochemical changes in plasma. Fifteen adult dogs had blood and urine samples obtained prior to, immediately and 4-hours following an agility exercise.
  
  
  RESULTS
  Hematocrit, red blood cells (RBC), albumin, and hemoglobin increased following exercise, with greatest increases correlating to increased skill group (novice, intermediate, masters); at 4-hours post-exercise, hematocrit, RBC, and hemoglobin were decreased. Phosphorus increased following exercise with the greatest increase in novice and intermediates. Plasma lactate increased 3.6-fold in masters, 3.2-fold in intermediates, and 1.2-fold in novice dogs. Urine thromboxane B2 (TXB2) more than tripled 4-hours post-exercise while 6-keto prostaglandin F1α (PGF1α, prostacyclin metabolite), prostaglandin E2 metabolites (13,14-dihydro-15-keto-prostaglandin A2 and 13,14-dihydro-15-keto-prostaglandin E2), and 13,14-dihydro-15-keto prostaglandin F2α were unaffected as determined by a competitive enzyme immunoassay and standardized by division with urine creatinine. Urine 15F2t-isoprostane increased insignificantly.
  
  
  CONCLUSIONS
  Alterations in the plasma post-exercise were likely due to hemoconcentration from insensible water loss, splenic contraction and sympathetic stimulation while 4-hours later autohemodilution reduced RBC parameters. Elevations in plasma lactate and urinary TXB2 correlated with advanced skill level/speed of the dogs.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA. wendy.baltzer@oregonstate.edu
authors.forename: Wendy I
authors.initials: WI
authors.lastname: Baltzer
authors.forename: Anna M
authors.initials: AM
authors.lastname: Firshman
authors.forename: Bernadette
authors.initials: B
authors.lastname: Stang
authors.forename: Jennifer J
authors.initials: JJ
authors.lastname: Warnock
authors.forename: Elena
authors.initials: E
authors.lastname: Gorman
authors.forename: Erica C
authors.initials: EC
authors.lastname: McKenzie
mesh_terms: D000818:Animals; D004285:Dogs / Q000097:blood / Q000502:physiology* / Q000652:urine; D015777:Eicosanoids / Q000652:urine*; D005260:Female; D019344:Lactic Acid / Q000097:blood* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000502:physiology; D008297:Male; D016877:Oxidants / Q000097:blood / Q000378:metabolism*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D013177:Sports; D013997:Time Factors
chemical_list: D015777:Eicosanoids; D016877:Oxidants; D019344:Lactic Acid