title: Nanoscale understanding of thermal aggregation of whey protein pretreated by transglutaminase.
abstract: Nanoscale structures of whey protein isolate (WPI) pretreated by microbial transglutaminase (mTGase) and subsequent heating were studied in this work and were correlated to zeta-potential, surface hydrophobicity, thermal denaturation properties, and macroscopic turbidity and viscosity. Dispersions of 5% w/v WPI were pretreated by individual or sequential steps of preheating at 80 °C for 15 min and mTGase, used at 2.0-10.2 U/g WPI for 1-15 h, before adjustment of the pH to 7.0 and to 0-100 mM NaCl for heating at 80 °C for 15 and 90 min. The zeta potential and surface hydrophobicity of WPI increased after all pretreatment steps. Preheating increased cross-linking reactivity of WPI by mTGase, corresponding to significantly increased denaturation temperature. Particle size analysis and atomic force microscopy revealed that structures of sequentially pretreated WPI remained stable after heating at 100 mM NaCl, corresponding to transparent dispersions. Conversely, WPI pretreated by one step aggregated at only 100 mM NaCl and resulted in turbid dispersions. Besides reporting a practical approach to produce transparent beverages, nanoscale phenomena in the present study are important for understanding whey protein structures in relevant applications.
authors.affiliation: Department of Food Science and Technology, The University of Tennessee, Knoxville, Tennessee 37996, USA.
authors.forename: Wan
authors.initials: W
authors.lastname: Wang
authors.forename: Qixin
authors.initials: Q
authors.lastname: Zhong
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Hu
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D001628:Beverages / Q000032:analysis; D004655:Emulsions; D006358:Hot Temperature; D008894:Milk Proteins / Q000737:chemistry / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011487:Protein Conformation; D055550:Protein Stability; D013499:Surface Properties; D013535:Suspensions; D011503:Transglutaminases / Q000378:metabolism*; D014783:Viscosity; D000067816:Whey Proteins
chemical_list: D001426:Bacterial Proteins; D004655:Emulsions; D008894:Milk Proteins; D013535:Suspensions; D000067816:Whey Proteins; D011503:Transglutaminases