title: [Cardiac protection from myocardial ischemic postconditioning and remote postconditioning during myocardial ischemia/reperfusion injury in rabbits].
abstract: OBJECTIVE
  To observe whether there are some differences between myocardial postconditioning and remote postconditioning, and whether there is additional cardiac protection when they are used combined during myocardial ischemia/reperfusion injury in rabbits.
  
  
  METHODS
  Thirty healthy New Zealand rabbits which were randomly divided into 5 groups (n = 5): ischemic control group (CON), sham operation group (Sham), myocardial postconditioning group (MPostC), remote postconditioning group (RPostC), myocardial postconditioning plus remote postconditioning group (MPostC + RPostC). Acute myocardial infarction was induced by 45 minutes occlusion on left circumflex coronary artery (LCX) and 2 hours reperfusion in all anesthetized open-chest rabbits except the Sham, the coronary occlusion and reperfusion were determined by changes of ECG and cardiac color. Skeletal muscle ischemia model was induced by extrinsic iliac arteries occlusion and reperfusion with artery clamps. The condition that the extrinsic iliac arteries were occluded or reperfused could be tested by according to the distal arterial pulse. Plasma creatine kinase (CPK) activity and lactate dehydrogenase (LDH) activity were measured at baseline, the end of ischemia, after 1 hour and 2 hours of reperfusion respectively. The extent of myocardial infarction was assessed by triphenyltetrazolium (TTC) staining and measured by area ratio of AN/AAR.
  
  
  RESULTS
  Compared with the Con, myocardial infarct size was significantly reduced in MPostC and RpostC group (P < 0.05). But there was no significant difference between MPostC and RPostC group. Combined MPostC and RPostC markedly enhanced myocardial protection (P < 0.05). The trend of CPK and LDH release was similar to the trend of myocardial infarct size.
  
  
  CONCLUSIONS
  Both MPostC and RPostC induced cardiac protection. There was no significant difference between MPostC and RPostC. Combined MPostC and RPostC induced markedly additive effect on myocardial protection.
authors.affiliation: Department of Cardiology, Second Hospital of Hebei Medical University, Shijiazhuang 055550, China.
authors.forename: Guang-Yun
authors.initials: GY
authors.lastname: Cao
authors.forename: Rui-Qin
authors.initials: RQ
authors.lastname: Xie
authors.forename: Wei
authors.initials: W
authors.lastname: Cui
authors.forename: Fan
authors.initials: F
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Jing-Chao
authors.initials: JC
authors.lastname: Lu
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Ren
authors.forename: Hai-Juan
authors.initials: HJ
authors.lastname: Hu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D057775:Ischemic Postconditioning; D018482:Muscle, Skeletal / Q000098:blood supply; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D009206:Myocardium / Q000378:metabolism; D011817:Rabbits