title: Listeria monocytogenes infection causes metabolic shifts in Drosophila melanogaster.
abstract: Immunity and metabolism are intimately linked; manipulating metabolism, either through diet or genetics, has the power to alter survival during infection. However, despite metabolism's powerful ability to alter the course of infections, little is known about what being "sick" means metabolically. Here we describe the metabolic changes occurring in a model system when Listeria monocytogenes causes a lethal infection in Drosophila melanogaster. L. monocytogenes infection alters energy metabolism; the flies gradually lose both of their energy stores, triglycerides and glycogen, and show decreases in both intermediate metabolites and enzyme message for the two main energy pathways, beta-oxidation and glycolysis. L. monocytogenes infection also causes enzymatic reduction in the anti-oxidant uric acid, and knocking out the enzyme uric oxidase has a complicated effect on immunity. Free amino acid levels also change during infection, including a drop in tyrosine levels which may be due to robust L. monocytogenes induced melanization.
authors.affiliation: Department of Microbiology and Immunology, Stanford University, Stanford, California, United States of America.
authors.forename: Moria C
authors.initials: MC
authors.lastname: Chambers
authors.forename: Kyung Han
authors.initials: KH
authors.lastname: Song
authors.forename: David S
authors.initials: DS
authors.lastname: Schneider
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D004331:Drosophila melanogaster; D005227:Fatty Acids / Q000378:metabolism; D005786:Gene Expression Regulation; D006003:Glycogen / Q000737:chemistry / Q000378:metabolism; D006019:Glycolysis; D007113:Immunity, Innate; D008089:Listeria monocytogenes / Q000378:metabolism*; D008088:Listeriosis / Q000276:immunology* / Q000378:metabolism*; D008543:Melanins / Q000378:metabolism; D055432:Metabolomics; D008954:Models, Biological; D009154:Mutation; D009711:Nucleotides / Q000235:genetics; D010100:Oxygen / Q000378:metabolism; D013234:Stem Cells; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism; D014527:Uric Acid / Q000737:chemistry
chemical_list: D000596:Amino Acids; D005227:Fatty Acids; D008543:Melanins; D009711:Nucleotides; D014280:Triglycerides; D014527:Uric Acid; D006003:Glycogen; D010100:Oxygen