title: Monitoring of monosaccharides, oligosaccharides, ethanol and glycerol during wort fermentation by biosensors, HPLC and spectrophotometry.
abstract: The aim of the present study was to analyze sugar levels (namely maltose, maltotriose, glucose and fructose) and alcohols (ethanol and glycerol) during the fermentation process in wort samples by amperometric enzymatic biosensors developed by our research group for industrial application, HPLC and spectrophotometry, and to compare the suitability of the presented methods for determination of individual analytes. We can conclude that for the specific monitoring of maltose or maltotriose only the HPLC method was suitable. On the other hand, biosensors and spectrophotometry reflected a decrease in total sugar concentration better and were able to detect both glucose and fructose in the later stages of fermentation, while HPLC was not. This can be attributed to the low detection limits and good sensitivity of the proposed methods. For the ethanol and glycerol analysis all methods proved to be suitable. However, concerning the cost expenses and time analysis, biosensors represented the best option.
authors.affiliation: Department of Nutrition and Food Assessment, Institute of Biochemistry, Nutrition and Health Protection, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovak Republic. rasto.monosik@gmail.com
authors.forename: Rastislav
authors.initials: R
authors.lastname: Monošík
authors.forename: Peter
authors.initials: P
authors.lastname: Magdolen
authors.forename: Miroslav
authors.initials: M
authors.lastname: Stredanský
authors.forename: Ernest
authors.initials: E
authors.lastname: Šturdík
mesh_terms: D000434:Alcoholic Beverages / Q000032:analysis* / Q000382:microbiology; D015374:Biosensing Techniques / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013053:Spectrophotometry / Q000379:methods*
chemical_list: D009844:Oligosaccharides; D000431:Ethanol; D005947:Glucose; D005990:Glycerol