title: MicroRNA-501 promotes HBV replication by targeting HBXIP.
abstract: MicroRNAs (miRNAs) can negatively regulate gene expression and also induce or inhibit viral replication. In the present study, we found 10 miRNAs were differentially expressed in a stable HBV-producing cell line (HepG2.2.15) compared with its control cell line (HepG2) by miRNA array analysis. miR-501 was significantly up-regulated in HepG2 cells and tissues with high-HBV replication. miR-501 expression was significantly up-regulated in hepatocellular carcinoma tissues, where HBV replication kept high. Down-regulating miR-501 could significantly inhibit HBV replication, but not influence the growth of HepG2.2.15 cells. Luciferase reporter and western blot assays revealed that HBXIP, an inhibitor of HBV replication, was a potential target of miR-501. Moreover, knockdown of HBXIP rescued the inhibition of HBV that occurred after the loss of miR-501 in HepG2.2.15 cells, suggesting that miR-501 induced HBV replication partially by targeting HBXIP. Thus, knockdown of miR-501 might provide a new mechanism and therapeutic target for inhibiting HBV replication.
authors.affiliation: State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, Shaanxi 710032, PR China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Jin
authors.forename: Shanhong
authors.initials: S
authors.lastname: Tang
authors.forename: Lin
authors.initials: L
authors.lastname: Xia
authors.forename: Rui
authors.initials: R
authors.lastname: Du
authors.forename: Huahong
authors.initials: H
authors.lastname: Xie
authors.forename: Jiugang
authors.initials: J
authors.lastname: Song
authors.forename: Rui
authors.initials: R
authors.lastname: Fan
authors.forename: Qian
authors.initials: Q
authors.lastname: Bi
authors.forename: Zheng
authors.initials: Z
authors.lastname: Chen
authors.forename: Guitao
authors.initials: G
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Shi
authors.forename: Daiming
authors.initials: D
authors.lastname: Fan
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D005260:Female; D055785:Gene Knockdown Techniques; D056945:Hep G2 Cells; D006509:Hepatitis B / Q000821:virology*; D006515:Hepatitis B virus / Q000502:physiology*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D014779:Virus Replication
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C110719:LAMTOR5 protein, human; C582676:MIRN501 microRNA, human; D035683:MicroRNAs