title: Lacrimal proline rich 4 (LPRR4) protein in the tear fluid is a potential biomarker of dry eye syndrome.
abstract: Dry eye syndrome (DES) is a complex, multifactorial, immune-associated disorder of the tear and ocular surface. DES with a high prevalence world over needs identification of potential biomarkers so as to understand not only the disease mechanism but also to identify drug targets. In this study we looked for differentially expressed proteins in tear samples of DES to arrive at characteristic biomarkers. As part of a prospective case-control study, tear specimen were collected using Schirmer strips from 129 dry eye cases and 73 age matched controls. 2D electrophoresis (2DE) and Differential gel electrophoresis (DIGE) was done to identify differentially expressed proteins. One of the differentially expressed protein in DES is lacrimal proline rich 4 protein (LPRR4). LPRR4 protein expression was quantified by enzyme immune sorbent assay (ELISA). LPRR4 was down regulated significantly in all types of dry eye cases, correlating with the disease severity as measured by clinical investigations. Further characterization of the protein is required to assess its therapeutic potential in DES.
authors.affiliation: Biochemistry and Cell Biology Department, Vision Research Foundation, Sankara Nethralaya, Chennai, Tamilnadu, India.
authors.forename: Saijyothi Venkata
authors.initials: SV
authors.lastname: Aluru
authors.forename: Shweta
authors.initials: S
authors.lastname: Agarwal
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Srinivasan
authors.forename: Geetha Krishnan
authors.initials: GK
authors.lastname: Iyer
authors.forename: Sivakumar M
authors.initials: SM
authors.lastname: Rajappa
authors.forename: Utpal
authors.initials: U
authors.lastname: Tatu
authors.forename: Prema
authors.initials: P
authors.lastname: Padmanabhan
authors.forename: Nirmala
authors.initials: N
authors.lastname: Subramanian
authors.forename: Angayarkanni
authors.initials: A
authors.lastname: Narayanasamy
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D015352:Dry Eye Syndromes / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013666:Tears / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D011506:Proteins; D012333:RNA, Messenger; C581527:lacrimal proline rich 4 protein, human