title: Apoptosis induction, cell cycle arrest and in vitro anticancer activity of gonothalamin in a cancer cell lines.
abstract: Cancer is one of the major health problems worldwide and its current treatments have a number of undesired adverse side effects. Natural compounds may reduce these. Currently, a few plant products are being used to treat cancer. In this study, goniothalamin, a natural occurring styryl-lactone extracted from Goniothalamus macrophyllus, was investigated for cytotoxic properties against cervical cancer (HeLa), breast carcinoma (MCF-7) and colon cancer (HT29) cells as well as normal mouse fibroblast (3T3) using MTT assay. Fluorescence microscopy showed that GTN is able to induce apoptosis in HeLa cells in a time dependent manner. Flow cytometry further revealed HeLa cells treated with GTN to be arrested in the S phase. Phosphatidyl serine properties present during apoptosis enable early detection of the apoptosis in the cells. Using annexin V/PI double staining it could be shown that GTN induces early apoptosis on HeLa cells after 24, 48 and 72 h. It could be concluded that goniothalamin showing a promising cytotoxicity effect against several cancer cell lines including cervical cancer cells (HeLa) with apoptosis as the mode of cell death induced on HeLa cells by Goniothalamin was.
authors.affiliation: Department of Biotechnology, Universiti Sultan Zainal Abidin (UniSZA), Kuala Terengganu, Malaysia. aied_absi@yahoo.com
authors.forename: Aied M
authors.initials: AM
authors.lastname: Alabsi
authors.forename: Rola
authors.initials: R
authors.lastname: Ali
authors.forename: Abdul Manaf
authors.initials: AM
authors.lastname: Ali
authors.forename: Sami Abdo Radman
authors.initials: SA
authors.lastname: Al-Dubai
authors.forename: Hazlan
authors.initials: H
authors.lastname: Harun
authors.forename: Noor H
authors.initials: NH
authors.lastname: Abu Kasim
authors.forename: Abdulsamad
authors.initials: A
authors.lastname: Alsalahi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000473:pathology; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D011753:Pyrones / Q000494:pharmacology*; D016196:S Phase / Q000187:drug effects*
chemical_list: D010936:Plant Extracts; D011753:Pyrones; C103280:goniothalamin