title: Haplotype variation of estrogen receptor-alpha (ER-alpha) gene exon 4 in Turkish sheep breeds.
abstract: Estrogen receptor a (ERalpha) gene has previously been found to related with sexual development and reproduction. In this study, on the basis of the sequences of human, cattle and caprine estrogen receptor a (ERalpha) genes, available in the GenBank database, sets of PCR primers were designed and used to amplify the ovine ERalpha gene exon 4 region. We identified six single nucleotide polymorphisms (SNP) in the ERalpha exon 4. Some variations determined for exon 4 g.43A > G, p.T43A; g.49C > T, p.L49F; g.178A > T, p.T178S led to changes in the amino acids, but no amino acid changes were determined in g. 18G > C, g.27C > T, g.96G > A. These fragments were deposited in the GenBank database under accession number: JF262030-JF262035. It was noted in particular that White Karaman and Awassi breeds were similar to each other, whereas the Chios breed had a different variation.
authors.affiliation: Firat University, Faculty of Veterinary, Medicine Department of Genetic, Elazig, Turkey. oozmen@firat.edu.tr
authors.forename: O
authors.initials: O
authors.lastname: Ozmen
authors.forename: I
authors.initials: I
authors.lastname: Seker
authors.forename: B C
authors.initials: BC
authors.lastname: Kul
authors.forename: O
authors.initials: O
authors.lastname: Ertugrul
mesh_terms: D000818:Animals; D001947:Breeding; D047628:Estrogen Receptor alpha / Q000235:genetics*; D005091:Exons; D006239:Haplotypes / Q000235:genetics*; D009154:Mutation; D034561:Sheep, Domestic / Q000235:genetics*; D014421:Turkey
chemical_list: D047628:Estrogen Receptor alpha