title: Bmcc1s interacts with the phosphate-activated glutaminase in the brain.
abstract: Bmcc1s, a brain-enriched short isoform of the BCH-domain containing molecule Bmcc1, has recently been shown to interact with the microtubule-associated protein MAP6 and to regulate cell morphology. Here we identified kidney-type glutaminase (KGA), the mitochondrial enzyme responsible for the conversion of glutamine to glutamate in neurons, as a novel partner of Bmcc1s. Co-immunoprecipitation experiments confirmed that Bmcc1s and KGA form a physiological complex in the brain, whereas binding and modeling studies showed that they interact with each other. Overexpression of Bmcc1s in mouse primary cortical neurons impaired proper mitochondrial targeting of KGA leading to its accumulation within the cytoplasm. Thus, Bmcc1s may control the trafficking of KGA to the mitochondria.
authors.affiliation: Collège de France, Center for Interdisciplinary Research in Biology (CIRB)/Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7241/Institut National de la Santè et de la Recherche Médicale U1050, 11 Place Marcelin Berthelot, 75231 Paris Cedex 05, France.
authors.forename: Anne-Cécile
authors.initials: AC
authors.lastname: Boulay
authors.forename: Silvia
authors.initials: S
authors.lastname: Burbassi
authors.forename: Hans-Kristian
authors.initials: HK
authors.lastname: Lorenzo
authors.forename: Damarys
authors.initials: D
authors.lastname: Loew
authors.forename: Pascal
authors.initials: P
authors.lastname: Ezan
authors.forename: Christian
authors.initials: C
authors.lastname: Giaume
authors.forename: Martine
authors.initials: M
authors.lastname: Cohen-Salmon
mesh_terms: D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D005972:Glutaminase / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007668:Kidney / Q000201:enzymology; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D008958:Models, Molecular; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000378:metabolism*; D010710:Phosphates / Q000494:pharmacology*; D011485:Protein Binding; D011487:Protein Conformation; D021381:Protein Transport / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; C560677:PRUNE2 protein, mouse; D010710:Phosphates; D005972:Glutaminase