title: Tubulin tyrosine ligase like 12, a TTLL family member with SET- and TTL-like domains and roles in histone and tubulin modifications and mitosis.
abstract: hTTLL12 is a member of the tubulin tyrosine ligase (TTL) family that is highly conserved in phylogeny. It has both SET-like and TTL-like domains, suggesting that it could have histone methylation and tubulin tyrosine ligase activities. Altered expression of hTTLL12 in human cells leads to specific changes in H4K20 trimethylation, and tubulin detyrosination, hTTLL12 does not catalyse histone methylation or tubulin tyrosination in vitro, as might be expected from the lack of critical amino acids in its SET-like and TTLL-like domains. hTTLL12 misexpression increases mitotic duration and chromosome numbers. These results suggest that hTTLL12 has non-catalytic functions related to tubulin and histone modification, which could be linked to its effects on mitosis and chromosome number stability.
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire, UMR 7104 CNRS UDS - U 964 INSERM, Illkirch, France.
authors.forename: Jan
authors.initials: J
authors.lastname: Brants
authors.forename: Kostyantyn
authors.initials: K
authors.lastname: Semenchenko
authors.forename: Christine
authors.initials: C
authors.lastname: Wasylyk
authors.forename: Aude
authors.initials: A
authors.lastname: Robert
authors.forename: Annaick
authors.initials: A
authors.lastname: Carles
authors.forename: Alberto
authors.initials: A
authors.lastname: Zambrano
authors.forename: Karine
authors.initials: K
authors.lastname: Pradeau-Aubreton
authors.forename: Catherine
authors.initials: C
authors.lastname: Birck
authors.forename: Jack A
authors.initials: JA
authors.lastname: Schalken
authors.forename: Olivier
authors.initials: O
authors.lastname: Poch
authors.forename: Jan
authors.initials: J
authors.lastname: de Mey
authors.forename: Bohdan
authors.initials: B
authors.lastname: Wasylyk
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D008745:Methylation; D008938:Mitosis / Q000502:physiology*; D010453:Peptide Synthases / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D010802:Phylogeny; D014404:Tubulin / Q000378:metabolism*
chemical_list: D006657:Histones; D014404:Tubulin; D010453:Peptide Synthases; C553200:TTLL12 protein, human