title: A study of circulating anti-CD25 antibodies in non-small cell lung cancer.
abstract: OBJECTIVE
  Tumors can trigger specific immune response to tumor-associated antigens but the precise mechanism remains unclear. Since regulatory T-lymphocytes (Treg) play a crucial role in controlling autoimmune responses, the present work was undertaken to test whether dysfunction of Treg cells could be involved in developing autoimmunity in patients with lung cancer.
  
  
  METHODS
  In this study, we developed an in-house enzyme-linked immunosorbent assay to test circulating anti-CD25 autoantibodies among 272 patients with non-small cell lung cancer (NSCLC) and 226 control subjects matched in age, gender and smoking history.
  
  
  RESULTS
  Mann-Whitney U test showed that the anti-CD25 IgG level was significantly higher in patients with NSCLC than control subjects (Z = -7.48, P < 0.001) while the anti-CD25 IgA level was not significantly changed in the patient group as compared with the control group (Z = -1.34, P = 0.181). Spearman correlation analysis failed to reveal a significant correlation between the levels of anti-CD25 IgG and IgA either in patients with NSCLC (r = -0.034, P = 0.578) or in control subjects (r = 0.055, P = 0.429). ROC analysis showed an AUC of 0.70 for anti-CD25 IgG, in which NSCLC at stage III had the highest AUC (0.75). The sensitivity against a specificity of >90 % was 35.0 % for anti-CD25 IgG assay with an inter-assay deviation of 9.4 %, and 4.0 % for anti-CD25 IgA assay with an inter-assay deviation of 13.0 %.
  
  
  CONCLUSIONS
  Circulating anti-CD25 IgG antibody may be a useful biomarker for prognosis of lung cancer.
authors.affiliation: Departments of Pulmonary Oncology and Pathology, The Third Affiliated Hospital of Harbin Medical University, Harbin, 150040, People's Republic of China.
authors.forename: L
authors.initials: L
authors.lastname: Ye
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Sun
authors.forename: S
authors.initials: S
authors.lastname: Guan
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.forename: X
authors.initials: X
authors.lastname: Guan
authors.forename: K-H
authors.initials: KH
authors.lastname: Lee
authors.forename: J
authors.initials: J
authors.lastname: Wei
authors.forename: B
authors.initials: B
authors.lastname: Liu
mesh_terms: D000888:Antibodies, Anti-Idiotypic / Q000097:blood* / Q000276:immunology; D014408:Biomarkers, Tumor / Q000097:blood / Q000276:immunology; D002289:Carcinoma, Non-Small-Cell Lung / Q000276:immunology* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology*; D008175:Lung Neoplasms / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged
chemical_list: D000888:Antibodies, Anti-Idiotypic; D014408:Biomarkers, Tumor; D053645:Interleukin-2 Receptor alpha Subunit; C048844:anti-IgG