title: Single systemic administration of Ag85B of mycobacteria DNA inhibits allergic airway inflammation in a mouse model of asthma.
abstract: The immune responses of T-helper (Th) and T-regulatory cells are thought to play a crucial role in the pathogenesis of allergic airway inflammation observed in asthma. The correction of immune response by these cells should be considered in the prevention and treatment of asthma. Native antigen 85B (Ag85B) of mycobacteria, which cross-reacts among mycobacteria species, may play an important biological role in host-pathogen interaction since it elicits various immune responses by activation of Th cells. The current study investigated the antiallergic inflammatory effects of DNA administration of Ag85B from Mycobacterium kansasii in a mouse model of asthma. Immunization of BALB/c mice with alum-adsorbed ovalbumin followed by aspiration with aerosolized ovalbumin resulted in the development of allergic airway inflammation. Administration of Ag85B DNA before the aerosolized ovalbumin challenge protected the mice from subsequent induction of allergic airway inflammation. Serum and bronchoalveolar lavage immunoglobulin E levels, extent of eosinophil infiltration, and levels of Th2-type cytokines in Ag85B DNA-administered mice were significantly lower than those in control plasmid-immunized mice, and levels of Th1-and T-regulatory-type cytokines were enhanced by Ag85B administration. The results of this study provide evidence for the potential utility of Ag85B DNA inoculation as a novel approach for the treatment of asthma.
authors.affiliation: Laboratory of Immunoregulation and Vaccine Research, Tsukuba Primate Research Center, National Institute of Biomedical Innovation, Tsukuba ; Division of Immunoregulation, Department of Molecular and Experimental Medicine, Mie University Graduate School of Medicine, Tsu.
authors.forename: Katsuo
authors.initials: K
authors.lastname: Karamatsu
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Matsuo
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Inada
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tsujimura
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Shiogama
authors.forename: Akihiro
authors.initials: A
authors.lastname: Matsubara
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Kawano
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yasutomi