title: Electroanalysis of single-nucleotide polymorphism by hairpin DNA architectures.
abstract: Genetic analysis of infectious and genetic diseases and cancer diagnostics require the development of efficient tools for fast and reliable analysis of single-nucleotide polymorphism (SNP) in targeted DNA and RNA sequences often responsible for signalling disease onset. Here, we highlight the main trends in the development of electrochemical genosensors for sensitive and selective detection of SNP that are based on hairpin DNA architectures exhibiting better SNP recognition properties compared with linear DNA probes. SNP detection by electrochemical hairpin DNA beacons is discussed, and comparative analysis of the existing SNP sensing strategies based on enzymatic and nanoparticle signal amplification schemes is presented.
authors.affiliation: Interdisciplinary Nanoscience Center, Aarhus University, Gustav Wieds Vej 1590-14, 8000 Aarhus C, Denmark.
authors.forename: Alireza
authors.initials: A
authors.lastname: Abi
authors.forename: Elena E
authors.initials: EE
authors.lastname: Ferapontova
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods*; D020641:Polymorphism, Single Nucleotide
chemical_list: D015342:DNA Probes; D004247:DNA