title: [Study on secondary metabolites of marine fungus Penicillium sp. FS60 from the South China Sea].
abstract: OBJECTIVE
  To study the secondary metabolites of the marine fungus Penicillium sp. FS60 from the South China Sea and their cytotoxicities.
  
  
  METHODS
  The compounds were isolated from the culture of strain FS60 by various chromatographic methods (silica gel, reverse silica gel, Sephadex-LH20, preparative TLC, HPLC and PTLC) and recrystallization. Their structures were identified by extensive analysis of their spectroscopic data. Compounds were tested for their cytotoxicities against SF-268, MCF-7, and NCI-H460 cell lines by SRB method. While, Compounds were tested for their antibacterial activities against S. aureus, E. coli and P. aeruginosa.
  
  
  RESULTS
  Seven compounds were isolated from the culture and identified as methyl 2,4-dihydroxy-3,5,6-trimethylbenzoate (1), 4-hydroxyacetophenone (2), 5-hydroxymethyl-furoic acid (3), isochromophilones VIII (4), ergosterol (5), ergosterol peroxide (6), and cerevisterol (7).
  
  
  CONCLUSIONS
  Compound 1 is isolated from the genus Penicillium for the first time. Compound 3 is demonstrated to have significant inhibition against S. aureus and P. aeruginosa. Compound 4 is demonstrated to have significant inhibition against the three cell lines.
authors.affiliation: Guangdong Provincial Open Laboratory of Applied Microbiology,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Institute of Microbiology, Guangzhou 510070, China. zhangling2006-22@163.com
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Dong-Li
authors.initials: DL
authors.lastname: Li
authors.forename: Yu-Chan
authors.initials: YC
authors.lastname: Chen
authors.forename: Mei-Hua
authors.initials: MH
authors.lastname: Tao
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Zhang
mesh_terms: D000098:Acetophenones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001578:Benzopyrans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002681:China; D004354:Drug Screening Assays, Antitumor; D005663:Furans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D015394:Molecular Structure; D009792:Oceans and Seas; D010407:Penicillium / Q000737:chemistry* / Q000378:metabolism
chemical_list: C521074:5-hydroxymethyl-2-furoic acid; D000098:Acetophenones; D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D001578:Benzopyrans; D005663:Furans; C031335:4-hydroxyacetophenone