title: Increased circulating red cell microparticles (RMP) and platelet microparticles (PMP) in immune thrombocytopenic purpura.
abstract: It has been suggested that patients with ITP have an increased thrombotic risk compared to the general population and compared to those with other causes of acquired thrombocytopenia. The pro-coagulant role of microparticles in some clinical situations has been reported, yet, very little data is available about microparticles in ITP and their effect.
  
  
  OBJECTIVE
  To assess the levels of red cell microparticles (RMP), platelet microparticles (PMP) and their possible relation to some haemostatic parameters in ITP patients
  
  
  METHODS
  The levels of RMP and PMP in addition to FVIII, FIX, FXI, PC and aPTT were assessed in 29 patients with chronic ITP (8 of them had splenectomy). Ten apparently healthy volunteers served as controls. We compared the levels of the studied parameters in ITP patients with that in controls. Correlations of these parameters with each other and with the platelet count were studied.
  
  
  RESULTS
  RMP (p=0.0001), PMP (p=0.0001), D- dimer (p=0.048), FVIII (p=0.049), FIX (p=0.0001) and FXI (p=0.0001) were significantly higher in ITP patients compared to controls. aPTT was significantly longer in ITP patients (p=0.0001) but PC showed no significant difference. However, RMP was associated with shorter aPTT. Generally, the coagulation factors were negatively correlated with platelet count in ITP patients. Compared to controls, ITP patients preserved higher levels of RMP and PMP even in those with near-normal platelet count. Splenectomy was associated with lower FIX (p=0.0001) and FXI (p=0.028) and higher RMP (p=0.0001).
  
  
  CONCLUSIONS
  Chronic ITP was associated with increased levels of RMP and PMP. FVIII, FIX and FXI were increased in ITP patients but showed a negative correlation with platelet count. Splenectomy was associated with increased levels of RMP and lower levels of FIX and F XI. The high level of microparticles in ITP might point towards a prothrombotic tendency.
authors.affiliation: Department of Internal Medicine, Faculty of Medicine, Assiut University, Egypt.
authors.forename: Eman M
authors.initials: EM
authors.lastname: Sewify
authors.forename: Douaa
authors.initials: D
authors.lastname: Sayed
authors.forename: Refat F
authors.initials: RF
authors.lastname: Abdel Aal
authors.forename: Heba M
authors.initials: HM
authors.lastname: Ahmad
authors.forename: Madleen A
authors.initials: MA
authors.lastname: Abdou
mesh_terms: D000293:Adolescent; D000328:Adult; D001792:Blood Platelets / Q000473:pathology*; D055252:Cell-Derived Microparticles / Q000473:pathology*; D004912:Erythrocytes / Q000473:pathology*; D006801:Humans; D008875:Middle Aged; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000097:blood*; D055815:Young Adult