title: Magnetic nanohydroxyapatite/PVA composite hydrogels for promoted osteoblast adhesion and proliferation.
abstract: This paper reports on the systematic investigation of novel magnetic nano-hydroxyapatite/PVA composite hydrogels through cyclic freeze-thawing with controllable structure, mechanical properties, and cell adhesion and proliferation properties. The content of the magnetic nano-hydroxyapatite-coated γ-Fe(2)O(3) (m-nHAP) particles exhibited remarkable influence on the porous structures and compressive strength of the nanocomposite hydrogels. The average pore diameter of the nanocomposite hydrogels exhibited a minimum of 1.6 ± 0.3 μm whereas the compressive strength reached a maximum of about 29.6 ± 6.5 MPa with the m-nHAP content of around 10 wt% in the nanocomposite hydrogels. In order to elucidate the influence of the composite m-nHAP on the cell adhesion and proliferation on the composite hydrogels, the PVA, γ-Fe(2)O(3)/PVA, nHAP/PVA and m-nHAP/PVA hydrogels were seeded and cultured with osteoblasts. The results demonstrated that the osteoblasts preferentially adhered to and proliferated on the m-nHAP/PVA hydrogels, in comparison to the PVA and nHAP/PVA hydrogels, whereas the γ-Fe(2)O(3)/PVA hydrogels appeared most favorable to the osteoblasts. Moreover, with the increasing m-nHAP content in the composite hydrogels, the adhesion density and proliferation of the osteoblasts were significantly promoted, especially at the content of around 50 wt%.
authors.affiliation: Polymers and Composites Division, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, PR China.
authors.forename: Ruixia
authors.initials: R
authors.lastname: Hou
authors.forename: Guohua
authors.initials: G
authors.lastname: Zhang
authors.forename: Gaolai
authors.initials: G
authors.lastname: Du
authors.forename: Danxia
authors.initials: D
authors.lastname: Zhan
authors.forename: Yang
authors.initials: Y
authors.lastname: Cong
authors.forename: Yajun
authors.initials: Y
authors.lastname: Cheng
authors.forename: Jun
authors.initials: J
authors.lastname: Fu
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D019245:Compressive Strength / Q000187:drug effects; D017886:Durapatite / Q000138:chemical synthesis / Q000494:pharmacology*; D052203:Ferrosoferric Oxide / Q000138:chemical synthesis; D006801:Humans; D020100:Hydrogels / Q000494:pharmacology*; D060328:Magnetic Phenomena; D008856:Microscopy, Fluorescence; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D011142:Polyvinyl Alcohol / Q000494:pharmacology*; D016062:Porosity / Q000187:drug effects; D014961:X-Ray Diffraction
chemical_list: D020100:Hydrogels; D011142:Polyvinyl Alcohol; D017886:Durapatite; D052203:Ferrosoferric Oxide