title: Expression of cell surface markers during self-renewal and differentiation of human adipose-derived stem cells.
abstract: Human adipose-derived stem cell populations express cell surface markers such as CD105, CD73, CD146 and CD140a/PDFGRα. However, it was unclear whether these markers could discriminate subpopulations of undifferentiated cells and whether the expression of these markers is modulated during differentiation. To address this issue, we analysed the immunophenotype of cultured human multipotent adipose derived stem (hMADS) cell populations at different adipocyte differentiation steps. We found that 100% of undifferentiated cells expressed CD73 and CD105. In contrast, CD146 and CD140a/PDFGRα marked two different subpopulations of cells. CD140a/PDGFRα subpopulation was regulated by FGF2, a critical factor of human adipose-derived stem cell self-renewal. During differentiation, CD73 was maintained and marked lipid-laden cells, whereas CD105 expression was inhibited in fully differentiated cells. The percentage of CD146 and CD140a/PDFGRα-positive cells declined as soon as cells had undergone differentiation. Altogether, these data support the notion that expanded adipose-derived stem cells are heterogeneous mixtures of cells and cell surface markers studied can discriminate subpopulations.
authors.affiliation: iBV, Institut Biology Valrose, University Nice Sophia Antipolis, Tour Pasteur, UFR Medicine, 28, avenue de Valombrose, 06189 Nice Cedex 2, France.
authors.forename: Tala
authors.initials: T
authors.lastname: Mohsen-Kanson
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Hafner
authors.forename: Brigitte
authors.initials: B
authors.lastname: Wdziekonski
authors.forename: Phi
authors.initials: P
authors.lastname: Villageois
authors.forename: Bérengère
authors.initials: B
authors.lastname: Chignon-Sicard
authors.forename: Christian
authors.initials: C
authors.lastname: Dani
mesh_terms: D015720:5'-Nucleotidase / Q000096:biosynthesis / Q000235:genetics; D017667:Adipocytes / Q000166:cytology*; D050156:Adipogenesis / Q000187:drug effects / Q000502:physiology*; D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects; D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics; D015415:Biomarkers / Q000378:metabolism; D051929:CD146 Antigen / Q000096:biosynthesis / Q000235:genetics; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D000071063:Endoglin; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism / Q000494:pharmacology; D005434:Flow Cytometry; D058851:GPI-Linked Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000096:biosynthesis / Q000235:genetics; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000235:genetics; D013234:Stem Cells / Q000166:cytology*
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D051929:CD146 Antigen; C064206:ENG protein, human; D000071063:Endoglin; D058851:GPI-Linked Proteins; C497902:MCAM protein, human; D011956:Receptors, Cell Surface; D016222:Fibroblast Growth Factor 2; D020796:Receptor, Platelet-Derived Growth Factor alpha; D015720:5'-Nucleotidase; C548241:NT5E protein, human