title: Spatiotemporal expression of the dermatopontin gene in zebrafish Danio rerio.
abstract: There has been significant interest in the expression and function of dermatopontin (DPT) in mammals owing to recent evidence pointing to its critical role in collagen fibrillogenesis. Despite this interest, limited information is available about the site/s of DPT mRNA expression or changes in expression in vivo. We used reverse-transcription polymerase chain reaction and in situ hybridization to evaluate the spatial and temporal pattern of DPT mRNA expression in zebrafish, Danio rerio, a widely used vertebrate model. We observed that DPT transcripts were expressed in zebrafish embryos at all developmental stages in a range of tissues, including the brain and optic neuron cells. Based on our results, we hypothesize that DPT may also play a role in neural functions in vivo.
authors.affiliation: Graduate School of Fisheries Science, Hokkaido University, Minato, Hakodate, Hokkaido, 0410821, Japan. tyk0011981@gmail.com
authors.forename: Yongkai
authors.initials: Y
authors.lastname: Tan
authors.forename: Kurin
authors.initials: K
authors.lastname: Iimura
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Sato
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Ura
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Takagi
mesh_terms: D000818:Animals; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D011508:Chondroitin Sulfate Proteoglycans / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004625:Embryo, Nonmammalian; D016326:Extracellular Matrix Proteins / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D008954:Models, Biological; D055495:Neurogenesis / Q000235:genetics / Q000502:physiology; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D014018:Tissue Distribution; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; D016326:Extracellular Matrix Proteins; D012333:RNA, Messenger; D029961:Zebrafish Proteins; C579291:dpt protein, zebrafish