title: Spatiotemporal analysis of organelle and macromolecular complex inheritance.
abstract: Following mitosis, daughter cells must inherit a functional set of essential proteins and organelles. We applied a genetic tool to simultaneously monitor the kinetics and distribution of old and new proteins marking all intracellular compartments in budding yeasts. Most organelles followed a general pattern whereby preexisting proteins are symmetrically partitioned followed by template-based incorporation of new proteins. Peroxisomes belong to this group, supporting a model of biogenesis by growth and division from preexisting peroxisomes. We detected two exceptions: the nuclear pore complex (NPC) and the spindle pole body (SPB). Old NPCs are stably inherited during successive generations but remained separated from new NPCs, which are incorporated de novo in mother and daughter cells. Only the SPB displayed asymmetrical distribution, with old components primarily inherited by daughter cells and new proteins equally incorporated in both cells. Our analysis resolves conflicting models (peroxisomes, NPC) and reveals unique patterns (NPC, SPB) of organelle inheritance.
authors.affiliation: Division of Cell Biology, Netherlands Cancer Institute and Netherlands Proteomics Centre, 1066 CX Amsterdam, The Netherlands. v.menendez@nki.nl
authors.forename: Victoria
authors.initials: V
authors.lastname: Menendez-Benito
authors.forename: Sjoerd J
authors.initials: SJ
authors.lastname: van Deventer
authors.forename: Victor
authors.initials: V
authors.lastname: Jimenez-Garcia
authors.forename: Marina
authors.initials: M
authors.lastname: Roy-Luzarraga
authors.forename: Fred
authors.initials: F
authors.lastname: van Leeuwen
authors.forename: Jacques
authors.initials: J
authors.lastname: Neefjes
mesh_terms: D002451:Cell Compartmentation / Q000502:physiology*; D008856:Microscopy, Fluorescence; D008938:Mitosis / Q000502:physiology*; D008954:Models, Biological; D022022:Nuclear Pore / Q000378:metabolism*; D015388:Organelles / Q000502:physiology*; D020675:Peroxisomes / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae; D008941:Spindle Apparatus / Q000378:metabolism*; D059008:Time-Lapse Imaging