title: [Construction of recombinant NF-κB reporter adenovirus and activity analysis].
abstract: OBJECTIVE
  To study the feasibility of adenovirus-based nuclear factor-κB (NF-κB) reporter as a model to screen the upstream signal regulators of  NF-κB.
  
  
  METHODS
  A type 5 (E1/E3 deficient) adenovirus vector pAdxsi was used to construct the NF-κB reporter adenovirus. Multiple adherent and  suspending cell lines were infected by the NF-κB reporter adenovirus, and the luciferase activity of the NF-κB reporter gene was measured.
  
  
  RESULTS
  An NF-κB reporter adenovirus (Ad-NF-κB-luc) was successfully constructed. The virus was capable of infecting HepG2, MGC803, THP-1 and U937  cell lines and showed high activities of NF-κB-luc reporter gene when stimulated by tumor necrosis factor-α (TNF-α) or lipopolysaccharide (LPS).
  
  
  CONCLUSIONS
  The Ad-NF-κB-luc reporter gene transfer system can effectively infect those cells hard-transfected by conventional transfection  reagents. It also produces a high activity of NF-κB-luc reporter gene with stability and reliability. Our study expands the application of NF-κB reporter  gene.
authors.affiliation: Peking University School of Basic Medical Sciences, Beijing, China.
authors.forename: Jin-hai
authors.initials: JH
authors.lastname: Guo
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Ping-zhang
authors.initials: PZ
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.forename: Tai-ping
authors.initials: TP
authors.lastname: Shi
authors.forename: Ying-yu
authors.initials: YY
authors.lastname: Chen
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D004274:DNA, Recombinant / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D017930:Genes, Reporter / Q000235:genetics*; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D016328:NF-kappa B / Q000235:genetics*; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D004274:DNA, Recombinant; D008070:Lipopolysaccharides; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha