title: Microbiological study of biofilm formation in isolates of Salmonella enterica Typhimurium DT104 and DT104b cultured from the modern pork chain.
abstract: The purpose of this study was to characterise 172 Salmonella Typhimurium isolates taken from the pork chain for their biofilm forming abilities and to analyse their potential to survive on food processing surfaces. Many Salmonella have the ability to form biofilms. These natural structures, elaborated by bacteria are important in food production because their formation contributes to bacterial survival. Adherent bacterial cells are more resilient to displacement strategies including physical and chemical procedures as a consequence of their altered more resistant phenotype. By improving our understanding of the nature of biofilms, this data could positively contribute to the development and implementation of eradication strategies. In this study, Salmonella Typhimurium DT104 and DT104b were investigated for their ability to form biofilms on a range of different surfaces under defined environmental growth conditions. Phenotypic characterisation involved examining colony morphology on indicator agars, assessing their ability to survive chlorine-based challenges and investigating their ability to attach to stainless steel and to plastic surfaces. All bacterial isolates were investigated for the presence of Salmonella genomic island I (SGI1) which is thought to enhance efficient biofilm formation. It was found that the majority of strains possess biofilm forming capabilities but successful attachment is highly dependent on the surface on which the biofilm is forming. The strains readily attached to stainless steel and plastic surfaces and survived high chlorine concentrations. Molecular and phenotypic comparisons of strong and weak biofilm forming strains indicate that biofilm development is not solely dependent on the acquirement of SGI1.
authors.affiliation: Food Safety Department, Teagasc Food Research Centre, Ashtown, Dublin 15, Ireland. denis.oleary@teagasc.ie
authors.forename: Denis
authors.initials: D
authors.lastname: O'Leary
authors.forename: Evonne M Mc
authors.initials: EM
authors.lastname: Cabe
authors.forename: Matthew P
authors.initials: MP
authors.lastname: McCusker
authors.forename: Marta
authors.initials: M
authors.lastname: Martins
authors.forename: Séamus
authors.initials: S
authors.lastname: Fanning
authors.forename: Geraldine
authors.initials: G
authors.lastname: Duffy
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology; D018441:Biofilms / Q000254:growth & development*; D004866:Equipment Contamination; D005511:Food Handling / Q000295:instrumentation; D044404:Genomic Islands / Q000235:genetics; D008460:Meat / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000235:genetics / Q000502:physiology*; D013193:Stainless Steel; D013552:Swine
chemical_list: D013193:Stainless Steel