title: Direct quantification of cell-associated HIV DNA in isolated rectal and blood memory CD4 T cells revealed their similar and low infection levels in long-term treated HIV-infected patients.
abstract: To evaluate the contribution of CD4 T cells from blood and gut compartments to the HIV-1 reservoir, we directly quantified cell-associated HIV DNA in isolated rectal (R-) and peripheral blood (PB-) memory CD4 T cells from 11 successfully long-term treated patients. Proportion of activated (CD25(+); CD69(+); and HLA-DR(+)) and CCR5 expressing CD4 T cells were markedly higher in rectal tissue compared with blood. However, HIV-1 infection levels of R- and PB-memory CD4 T cells did not significantly differ (medians: 4000 and 2100 copies per million cells) after effective long-term viral control, suggesting that each of these 2 compartments does not contribute in a similar fashion to the total HIV reservoir.
authors.affiliation: UPMC University Paris 06, INSERM, UMRS945, AP-HP, Hôpital Pitié-Salpêtrière, Laboratoire d'immunologie cellulaire et tissulaire, Paris, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Descours
authors.forename: Sidonie
authors.initials: S
authors.lastname: Lambert-Niclot
authors.forename: Benoît
authors.initials: B
authors.lastname: Mory
authors.forename: Assia
authors.initials: A
authors.lastname: Samri
authors.forename: Fréderic
authors.initials: F
authors.lastname: Charlotte
authors.forename: Gilles
authors.initials: G
authors.lastname: Peytavin
authors.forename: Roland
authors.initials: R
authors.lastname: Tubiana
authors.forename: Laura
authors.initials: L
authors.lastname: Papagno
authors.forename: Charline
authors.initials: C
authors.lastname: Bacchus
authors.forename: François
authors.initials: F
authors.lastname: Lecardonnel
authors.forename: Christine
authors.initials: C
authors.lastname: Katlama
authors.forename: Brigitte
authors.initials: B
authors.lastname: Autran
authors.forename: Anne-Geneviève
authors.initials: AG
authors.lastname: Marcelin
authors.forename: Marc-Antoine
authors.initials: MA
authors.lastname: Valantin
authors.forename: Guislaine
authors.initials: G
authors.lastname: Carcelain
mesh_terms: D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D004197:Disease Reservoirs / Q000821:virology*; D015658:HIV Infections / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000235:genetics*; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology; D019713:Receptors, CCR5 / Q000378:metabolism; D019562:Viral Load
chemical_list: D004279:DNA, Viral; D006684:HLA-DR Antigens; D019713:Receptors, CCR5