title: Transepithelial transport of 6-O-caffeoylsophorose across Caco-2 cell monolayers.
abstract: The aim of this study was to clarify the transport behaviour and mechanism of caffeic acid analogue bearing a sugar-moiety, 6-O-caffeoylsophorose (CS), in Caco-2 cells. The absorption of CS was investigated by its transport across Caco-2 cell monolayers using a high-performance liquid chromatography-time-of-flight-mass spectrometry (LC-TOF-MS). The permeation of CS was concentration-dependent and reached the plateau at >6 mM. The apparent permeability (P(app)) of CS in the apical-to-basolateral direction was 5.4×10(-7) cm/s, while in the reversed direction the P(app) value was significantly reduced (1.9×10(-7) cm/s). CS transport was competitively inhibited by phloretin, an inhibitor of monocarboxylic acid transporter (MCT). Benzoic acid, an MCT substrate, also reduced CS transport. A less significant change of CS transport was observed across Caco-2 cell monolayers pretreated with quercetin, a suppressor of tight-junction. These findings strongly indicate that CS, a caffeic acid analogue bearing sophorose moiety, can be transported across Caco-2 cell monolayers via the MCT pathway.
authors.affiliation: Department of Bioscience and Biotechnology, Division of Bioresource and Bioenviromental Sciences, Faculty of Agriculture, Graduated School of Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
authors.forename: Hoang Lan
authors.initials: HL
authors.lastname: Phuong
authors.forename: Ju
authors.initials: J
authors.lastname: Qiu
authors.forename: Takashi
authors.initials: T
authors.lastname: Kuwahara
authors.forename: Keiichi
authors.initials: K
authors.lastname: Fukui
authors.forename: Kayo
authors.initials: K
authors.lastname: Yoshiyama
authors.forename: Kazusato
authors.initials: K
authors.lastname: Matsugano
authors.forename: Norihiko
authors.initials: N
authors.lastname: Terahara
authors.forename: Toshiro
authors.initials: T
authors.lastname: Matsui
mesh_terms: D001692:Biological Transport; D018938:Caco-2 Cells; D002109:Caffeic Acids / Q000737:chemistry*; D004847:Epithelial Cells / Q000378:metabolism*; D006801:Humans; D027501:Monocarboxylic Acid Transporters / Q000378:metabolism; D010539:Permeability
chemical_list: D002109:Caffeic Acids; D027501:Monocarboxylic Acid Transporters; C040048:caffeic acid