title: [Determination of vitexin in plasma by HPLC-MS/MS method and its pharmacokinetics in rats].
abstract: OBJECTIVE
  To establish a HPLC-MS/MS method for the determination of vitexin in rat plasma and its pharmacokinetics.
  
  
  METHODS
  The HPLC-MS/MS method used Capcell Pak C18 column (50 mm x 2.0 mm I. D., 5 microm). The mobile phase was methanol and water (95:5, V/V, containing 0.1% formic acid) at a flow rate of 0.2 mL/min. Electrospray ionization (ESI) in negative ion mode and multiple reaction monitoring (MRM) was used for the quantification of vitexin with a monitored transitions m/z 431-->311 for vitexin and m/z 269-->225 for internal standard (I. S., emodin).
  
  
  RESULTS
  Linear calibration curves were obtained over the concentration range of 0.5-2000 ng/mL (r = 0.9960) with the lowest limit of quantification (LLOQ) of 0.5 ng/mL. The recovery was in the range of 76.1%-89.0%. The relative standard deviations for the intra-day and inter-day validation were less than 11%.
  
  
  CONCLUSIONS
  The method is simple, accurate, fast, sensitive and suitable for the pharmacokinetic study of vitexin in rats.
authors.affiliation: Department of Traditional Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou 510006, China. cuishengmiao@yahoo.com
authors.forename: Sheng-Miao
authors.initials: SM
authors.lastname: Cui
authors.forename: Xiu-Fang
authors.initials: XF
authors.lastname: Wei
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhen-Zhen
authors.initials: ZZ
authors.lastname: Ye
authors.forename: Hua-Wei
authors.initials: HW
authors.lastname: Liao
mesh_terms: D000284:Administration, Oral; D000818:Animals; D047310:Apigenin / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D027825:Crataegus / Q000737:chemistry*; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010936:Plant Extracts; D047310:Apigenin; C032731:vitexin