title: Cab45 is required for Ca(2+)-dependent secretory cargo sorting at the trans-Golgi network.
abstract: Ca(2+) import into the lumen of the trans-Golgi network (TGN) by the secretory pathway calcium ATPase1 (SPCA1) is required for the sorting of secretory cargo. How is Ca(2+) retained in the lumen of the Golgi, and what is its role in cargo sorting? We show here that a soluble, lumenal Golgi resident protein, Cab45, is required for SPCA1-dependent Ca(2+) import into the TGN; it binds secretory cargo in a Ca(2+)-dependent reaction and is required for its sorting at the TGN.
authors.affiliation: Max Planck Institute for Biochemistry, 82152 Martinsried, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: von Blume
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Alleaume
authors.forename: Christine
authors.initials: C
authors.lastname: Kienzle
authors.forename: Amado
authors.initials: A
authors.lastname: Carreras-Sureda
authors.forename: Miguel
authors.initials: M
authors.lastname: Valverde
authors.forename: Vivek
authors.initials: V
authors.lastname: Malhotra
mesh_terms: D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D064236:Cartilage Oligomeric Matrix Protein; D002402:Cathepsin D / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D055785:Gene Knockdown Techniques; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006706:Homeostasis; D006801:Humans; D064235:Matrilin Proteins; D011485:Protein Binding; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D034622:RNA Interference; D055571:Secretory Pathway; D021601:trans-Golgi Network / Q000378:metabolism*
chemical_list: D002135:Calcium-Binding Proteins; D064236:Cartilage Oligomeric Matrix Protein; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D064235:Matrilin Proteins; D020033:Protein Isoforms; C577553:TSP5 protein, human; C099100:SDF4 protein, human; C533922:CTSD protein, human; D002402:Cathepsin D; D002118:Calcium