title: Matrix metalloproteinase-8 and interleukin-1beta in gingival fluid of children in the first three months of orthodontic treatment with fixed appliances.
abstract: In orthodontic treatment remodeling of periodontal space is concomitant with the movement of teeth. Matrix metalloproteinase-8 and interleukin-1beta are key markers of the initial tissue reaction in the processes of remodeling. The gingival fluid is the medium where changes in the profile and levels of these mediators occur. The AIM of this study was to investigate the levels of matrix metalloproteinase-8 and interleukin-1beta in gingival fluid samples during the first 3 months of orthodontic treatment with fixed appliances in children.
  
  
  METHODS
  Twelve children receiving brackets treatment were included in the study; 48 samples of gingival fluid collected from one representative tooth of these children were measured once before placing the brackets, then at 24 hours, at 1 week, and at 3 months. We measured the amount of gingival fluid and the levels of matrix metalloproteinase-8 and interleukin-1beta. Filter paper strips were used to collect gingival fluid. After eluting them we made a quantitative analysis of the biomarkers, matrix metalloproteinase-8 and interleukin-1beta, using the solid-phase enzyme immunoassay (ELISA). The results showed a slight drop in the levels of both markers compared with baseline values and an increase at three months of orthodontic treatment. A similar tendency was observed in the flow of gingival fluid.
  
  
  CONCLUSIONS
  Quantitative analysis of matrix metalloproteinase-8 and interleukin-1beta in gingival fluid samples is potentially a non-invasive method by which orthodontists can get information about the remodeling processes in the periodontium during orthodontic treatment thus controlling and limiting them within physiological boundaries.
authors.affiliation: Department of Pediatric Dentistry, Faculty of Dental Medicine, Medical University, Sofia, Bulgaria.
authors.forename: Lora S
authors.initials: LS
authors.lastname: Ribagin
authors.forename: Maya R
authors.initials: MR
authors.lastname: Rashkova
mesh_terms: D000293:Adolescent; D002648:Child; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism*; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000378:metabolism*; D009967:Orthodontic Appliances; D009971:Orthodontics, Corrective
chemical_list: D053583:Interleukin-1beta; D020784:Matrix Metalloproteinase 8