title: Analysis of five rice 4-coumarate:coenzyme A ligase enzyme activity and stress response for potential roles in lignin and flavonoid biosynthesis in rice.
abstract: 4-Coumarate:coenzyme A ligase (4CL) catalyzes the conversion of hydroxycinnamates into corresponding CoA esters for biosynthesis of flavonoids and lignin. In this study, five members of the 4CL gene family from rice were cloned and analyzed. Recombinant 4CL data revealed that 4-coumaric acid and ferulic acid were the two main substrates of 4CL (Os4CL1/3/4/5) for monolignol biosynthesis in rice. Os4CL2 was specifically expressed in the anther and was strongly activated by UV irradiation, suggesting its potential involvement in flavonoid formation. Moreover, bioinformatics analysis showed that the existence of valine residue at the substrate-binding pocket may mainly affect rice 4CL activities toward sinapic acid.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Sun
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Shengqiu
authors.initials: S
authors.lastname: Feng
authors.forename: Weihua
authors.initials: W
authors.lastname: Zou
authors.forename: Kai
authors.initials: K
authors.lastname: Guo
authors.forename: Chunfen
authors.initials: C
authors.lastname: Fan
authors.forename: Shengli
authors.initials: S
authors.lastname: Si
authors.forename: Liangcai
authors.initials: L
authors.lastname: Peng
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D003066:Coenzyme A Ligases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005419:Flavonoids / Q000096:biosynthesis*; D018506:Gene Expression Regulation, Plant; D007700:Kinetics; D008031:Lignin / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology* / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D005419:Flavonoids; D008031:Lignin; D003066:Coenzyme A Ligases; C019943:4-coumarate-CoA ligase