title: Identification of an optimized panel of variable number tandem-repeat (VNTR) loci for Listeria monocytogenes typing.
abstract: Listeria monocytogenes is an important worldwide foodborne pathogen. For listeriosis outbreak investigations, an optimal multiple-locus variable-number-tandem-repeat analysis typing panel was identified from 4 previously reported schemes. The optimal combination of loci consisted of 9 loci (LMV6, LMV1, LMV2, Lm11, Lm10, LMV7, Lm32, LM-TR6, and Lm23), which produced the same level of differentiation ability Simpson index of 0.9914 as that of all 14 loci combined in the previous 4 reports. This panel provided higher differentiation ability than the individual of the 4 previously reported schemes, suggesting it would be useful for future surveillance and outbreak investigations.
authors.affiliation: Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang, Hebei Province, China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Li
authors.forename: Bixing
authors.initials: B
authors.lastname: Huang
authors.forename: Sofroni
authors.initials: S
authors.lastname: Eglezos
authors.forename: Trudy
authors.initials: T
authors.lastname: Graham
authors.forename: Barry
authors.initials: B
authors.lastname: Blair
authors.forename: John
authors.initials: J
authors.lastname: Bates
mesh_terms: D000818:Animals; D002645:Chickens; D004269:DNA, Bacterial / Q000032:analysis; D005517:Foodborne Diseases / Q000382:microbiology; D005798:Genes, Bacterial; D006801:Humans; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008088:Listeriosis / Q000382:microbiology; D008460:Meat / Q000382:microbiology; D018598:Minisatellite Repeats; D058889:Molecular Typing / Q000379:methods*; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial