title: Determination of nimetazepam and 7-aminonimetazepam in human urine by using liquid chromatography-tandem mass spectrometry.
abstract: We report determination of metabolites of popular drugs of abuse, including nimetazepam and nitrazepam, in urine by using liquid chromatography/mass spectrometry. Nimetazepam and its metabolites, 7-aminonimetazepam and nitrazepam, were extracted by solid-phase extraction using a DAU cartridge. An ammonium acetate buffer solution (pH 4) and a Luna polar-RP column were selected as the mobile and stationary phase, respectively, for liquid chromatography. Mass spectrometry was used for analysis and was optimized for operation in the positive mode for all analytes. The urine specimens were screened for the presence of nimetazepam and its metabolites nitrazepam and 7-aminonimetazepam at a concentration of 0.1ng/mL. Presence of 7-aminonimetazepam in the urine was an indicator of the subject being a probable abuser of nimetazepam.
authors.affiliation: School of Pharmacy, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan, ROC.
authors.forename: Kuang-Chuan
authors.initials: KC
authors.lastname: Wang
authors.forename: Min-Chi
authors.initials: MC
authors.lastname: Cheng
authors.forename: Chin-Lin
authors.initials: CL
authors.lastname: Hsieh
authors.forename: Jung-Fa
authors.initials: JF
authors.lastname: Hsu
authors.forename: Jen-Der
authors.initials: JD
authors.lastname: Wu
authors.forename: Ching-Kuo
authors.initials: CK
authors.lastname: Lee
mesh_terms: D002853:Chromatography, Liquid; D053593:Forensic Toxicology; D006801:Humans; D006993:Hypnotics and Sedatives / Q000737:chemistry / Q000652:urine*; D015394:Molecular Structure; D009567:Nitrazepam / Q000031:analogs & derivatives* / Q000737:chemistry / Q000652:urine; D019966:Substance-Related Disorders / Q000175:diagnosis; D053719:Tandem Mass Spectrometry
chemical_list: C582339:7-aminonimetazepam; D006993:Hypnotics and Sedatives; C002714:nimetazepam; D009567:Nitrazepam