title: Two new natural products from the fruits of Alpinia oxyphylla with inhibitory effects on nitric oxide production in lipopolysaccharide-activated RAW264.7 macrophage cells.
abstract: Chemical investigation of the fruits of Alpinia oxyphylla led to an isolation of the two new natural products, 9-hydroxy epinootkatol (1) and (S)-2-pentanol-2-O-β-D-glucopyranoside (2), in addition to the nine known compounds, pinocembrin (3), tectochrysin (4), izalpinin (5), nookatone (6), yakuchinone A (7), protocatechuic acid (8), β-sitosterol (9), daucosterol (10) and β-sitosterol palmitate (11). Their structures were elucidated on the basis of physicochemical constants and NMR spectral data analysis. The effects of the isolated components on nitric oxide production in LPS-induced RAW 264.7 macrophages were examined. The two new natural compounds showed inhibitory activities with IC(50) values of 21.8 and 32.8 μg/mL, respectively.
authors.affiliation: Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, China.
authors.forename: Zhang Jun
authors.initials: ZJ
authors.lastname: Qing
authors.forename: Wang
authors.initials: W
authors.lastname: Yong
authors.forename: Li Yong
authors.initials: LY
authors.lastname: Hui
authors.forename: Lai Wei
authors.initials: LW
authors.lastname: Yong
authors.forename: Li Hai
authors.initials: LH
authors.lastname: Long
authors.forename: Duan Jin
authors.initials: DJ
authors.lastname: Ao
authors.forename: Pei Li
authors.initials: PL
authors.lastname: Xia
mesh_terms: D032429:Alpinia; D000818:Animals; D001688:Biological Products / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D005638:Fruit; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000633:toxicity; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D045787:Sesquiterpenes, Eudesmane / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: C000608514:9-hydroxyepinootkatol; D001688:Biological Products; D008070:Lipopolysaccharides; D010936:Plant Extracts; D045787:Sesquiterpenes, Eudesmane; D009569:Nitric Oxide