title: Neuroligin-1 controls synaptic abundance of NMDA-type glutamate receptors through extracellular coupling.
abstract: Despite the pivotal functions of the NMDA receptor (NMDAR) for neural circuit development and synaptic plasticity, the molecular mechanisms underlying the dynamics of NMDAR trafficking are poorly understood. The cell adhesion molecule neuroligin-1 (NL1) modifies NMDAR-dependent synaptic transmission and synaptic plasticity, but it is unclear whether NL1 controls synaptic accumulation or function of the receptors. Here, we provide evidence that NL1 regulates the abundance of NMDARs at postsynaptic sites. This function relies on extracellular, NL1 isoform-specific sequences that facilitate biochemical interactions between NL1 and the NMDAR GluN1 subunit. Our work uncovers NL1 isoform-specific cis-interactions with ionotropic glutamate receptors as a key mechanism for controlling synaptic properties.
authors.affiliation: Biozentrum,  University of Basel, 4056 Basel,  Switzerland.
authors.forename: Elaine C
authors.initials: EC
authors.lastname: Budreck
authors.forename: Oh-Bin
authors.initials: OB
authors.lastname: Kwon
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Jung
authors.forename: Stephane
authors.initials: S
authors.lastname: Baudouin
authors.forename: Albert
authors.initials: A
authors.lastname: Thommen
authors.forename: Hye-Sun
authors.initials: HS
authors.lastname: Kim
authors.forename: Yugo
authors.initials: Y
authors.lastname: Fukazawa
authors.forename: Harumi
authors.initials: H
authors.lastname: Harada
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Tabuchi
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Shigemoto
authors.forename: Peter
authors.initials: P
authors.lastname: Scheiffele
authors.forename: Joung-Hun
authors.initials: JH
authors.lastname: Kim
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism*; D016291:Dizocilpine Maleate; D047468:Immunoprecipitation; D018613:Microscopy, Confocal; D016253:Microscopy, Immunoelectron; D009473:Neuronal Plasticity / Q000502:physiology*; D051381:Rats; D058468:Receptors, Ionotropic Glutamate / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D018709:Statistics, Nonparametric; D013569:Synapses / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology*
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D058468:Receptors, Ionotropic Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; C093438:neuroligin 1; D016291:Dizocilpine Maleate