title: Efficient sequential repetitive gene deletions in Neurospora crassa employing a self-excising β-recombinase/six cassette.
abstract: Despite its long-standing history as a model organism, Neurospora crassa has limited tools for repetitive gene deletions utilizing recyclable self-excising marker systems. Here we describe, for the first time, the functionality of a bacterial recombination system employing β-recombinase acting on six recognition sequences (β-rec/six) in N. crassa, which allowed repetitive site-specific gene deletion and marker recycling. We report generating the mus-51 deletion strain using this system, recycling the marker cassette, and subsequently deleting the global transcriptional regulator gene cre-1.
authors.affiliation: Department of Biological and Agricultural Engineering, University of California, Davis, Davis, CA 95616, USA.
authors.forename: Edyta
authors.initials: E
authors.lastname: Szewczyk
authors.forename: Takao
authors.initials: T
authors.lastname: Kasuga
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Fan
mesh_terms: D017353:Gene Deletion; D018390:Gene Targeting / Q000379:methods; D005827:Genetics, Microbial / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D009492:Neurospora crassa / Q000235:genetics*; D045522:Recombinases / Q000378:metabolism*
chemical_list: D045522:Recombinases