title: Early hepatic and peritoneal changes and immune response in goats vaccinated with a recombinant glutathione transferase sigma class and challenged with Fasciola hepatica.
abstract: Changes and local immune response were evaluated in the peritoneal cell populations, duodenal lamina propria and liver from goats immunized with recombinant glutathione transferase sigma class (rFhGST-S1) during early stages of infection with Fasciola hepatica. Group 1 (n=7) was unimmunized and uninfected; group 2 (n=10) was immunized with adjuvant Quil A and infected; group 3 (n=10) was immunised with rFhGST-S1 and infected. Three goats from each group were killed at 7-9 days post-infection (dpi) to evaluate early changes and immune response. The remaining goats were killed at 15 weeks post-infection (wpi). rFhGST-S1 vaccination induced variable response: three goats showed low fluke burden at 15 wpi and two goats showed low hepatic damage at early infection stages. This response was associated to a severe infiltrate of eosinophils in peritoneal fluid and hepatic necrotic foci, high iNOS expression in peritoneal cells and abundant infiltrate of eosinophils surrounding hepatic migrating flukes. T lymphocyte subsets were found in the vicinity of necrotic areas but they were absent in the vicinity of migrating larvae. No significant variation for T cell subsets, except for CD4 and γδ T lymphocytes, that were higher in the Quil A group compared to the rFhGST-S1 group. Expression of IL4 and IFN-γ in the hepatic inflammatory infiltrates was very occasional.
authors.affiliation: Department of Anatomy and Comparative Pathology, School of Veterinary Medicine, University of Cordoba, Animal Health Building, Campus Rabanales, Cordoba, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Zafra
authors.forename: R A
authors.initials: RA
authors.lastname: Pérez-Écija
authors.forename: L
authors.initials: L
authors.lastname: Buffoni
authors.forename: I L
authors.initials: IL
authors.lastname: Pacheco
authors.forename: A
authors.initials: A
authors.lastname: Martínez-Moreno
authors.forename: E J
authors.initials: EJ
authors.lastname: LaCourse
authors.forename: S
authors.initials: S
authors.lastname: Perally
authors.forename: P M
authors.initials: PM
authors.lastname: Brophy
authors.forename: J
authors.initials: J
authors.lastname: Pérez
mesh_terms: D000276:Adjuvants, Immunologic / Q000627:therapeutic use; D000818:Animals; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000276:immunology / Q000469:parasitology / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D005982:Glutathione Transferase / Q000235:genetics / Q000276:immunology; D015511:Goat Diseases / Q000276:immunology / Q000469:parasitology* / Q000473:pathology / Q000517:prevention & control; D006041:Goats / Q000276:immunology / Q000469:parasitology; D007111:Immunity, Cellular / Q000276:immunology; D056724:Immunity, Humoral / Q000276:immunology; D008099:Liver / Q000469:parasitology / Q000473:pathology*; D008297:Male; D010537:Peritoneum / Q000469:parasitology / Q000473:pathology*; D062605:Quillaja Saponins; D012503:Saponins / Q000627:therapeutic use; D014612:Vaccines / Q000276:immunology* / Q000627:therapeutic use; D014614:Vaccines, Synthetic / Q000276:immunology / Q000627:therapeutic use
chemical_list: D000276:Adjuvants, Immunologic; D062605:Quillaja Saponins; D012503:Saponins; D014612:Vaccines; D014614:Vaccines, Synthetic; C046386:Quil A; D005982:Glutathione Transferase