title: Positive association of the vascular endothelial growth factor-A +405 GG genotype and poor survival in stage I-II gastric cancer in the Northern Chinese population.
abstract: The vascular endothelial growth factor-A (VEGF-A) plays an important role in the angiogenesis and prognosis for gastric cancer. In addition, several single-nucleotide polymorphisms (SNPs) in VEGF-A have been shown to affect gene expression and process of angiogenesis. The present study evaluated the correlations between SNPs in VEGF-A and gastric cancer survival. Formalin-fixed paraffin-embedded tissues of 404 gastric cancer patients and blood samples from 404 controls were included in the study. The SNPs -460T/C (rs833061), +405G/C (rs2101963), -7C/T (rs25648) and +936C/T (rs3025039) were genotyped by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. We showed patients carrying the +405GG genotype had significantly worse survival in the N0 (lymph-node negative), N0L0 (lymph-node negative and lymphovascular-invasion negative) and TNM stage I-II subgroups (P = 0.021, P = 0.007 and P = 0.017, respectively). In addition, haplotype -460T/+405G/-7C and -460C/+405G/-7C carriers showed poor survival in the N0, N0L0 and TNM stage I-II subgroups (P = 0.004, P = 0.030 and P = 0.009 for TGC; P = 0.033, P = 0.049 and P = 0.011 for CGC). Overall, the +405 GG genotype or TGC and CGC haplotypes were correlated with poor survival in TNM stage I-II gastric cancer patients. The +405G/C polymorphism of VEGF-A could be used as a biomarker for molecular staging in stage I-II gastric cancer in Northern China.
authors.affiliation: Department of Surgical Oncology and General Surgery, The First Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang City, 110001, China.
authors.forename: Ailin
authors.initials: A
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.forename: Zhenning
authors.initials: Z
authors.lastname: Wang
authors.forename: Yongxi
authors.initials: Y
authors.lastname: Song
authors.forename: Yingying
authors.initials: Y
authors.lastname: Xu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Miao
authors.forename: Jinliang
authors.initials: J
authors.lastname: Zhu
authors.forename: Huimian
authors.initials: H
authors.lastname: Xu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D005260:Female; D023061:Gene Order; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D020641:Polymorphism, Single Nucleotide; D013274:Stomach Neoplasms / Q000235:genetics* / Q000401:mortality* / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D042461:Vascular Endothelial Growth Factor A