title: Qualitative and quantitative expression status of the human chromosome 20 genes in cancer tissues and the representative cell lines.
abstract: Under the guidance of the Chromosome-centric Human Proteome Project (C-HPP), (1, 2)  we conducted a systematic survey of the expression status of genes located at human chromosome 20 (Chr.20) in three cancer tissues, gastric, colon, and liver carcinoma, and their representative cell lines. We have globally profiled proteomes in these samples with combined technology of LC-MS/MS and acquired the corresponding mRNA information upon RNA-seq and RNAchip. In total, 323 unique proteins were identified, covering 60% of the coding genes (323/547) in Chr.20. With regards to qualitative information of proteomics, we overall evaluated the correlation of the identified Chr.20 proteins with target genes of transcription factors or of microRNA, conserved genes and cancer-related genes. As for quantitative information, the expression abundances of Chr.20 genes were found to be almost consistent in both tissues and cell lines of mRNA in all individual chromosome regions, whereas those of Chr.20 proteins in cells are different from tissues, especially in the region of 20q13.33. Furthermore, the abundances of Chr.20 proteins were hierarchically evaluated according to tissue- or cancer-related distribution. The analysis revealed several cancer-related proteins in Chr.20 are tissue- or cell-type dependent. With integration of all the acquired data, for the first time we established a solid database of the Chr.20 proteome.
authors.affiliation: Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101318, China.
authors.forename: Quanhui
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authors.lastname: Wang
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authors.forename: Guangrong
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authors.lastname: Yan
authors.forename: Junying
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authors.lastname: Wei
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authors.forename: Shaohang
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authors.forename: Tingyou
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authors.initials: H
authors.lastname: Zhao
authors.forename: Zhe
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authors.lastname: Ren
authors.forename: Nengrong
authors.initials: N
authors.lastname: Qu
authors.forename: Xiaomin
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authors.forename: Haidan
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authors.forename: Chaoqin
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authors.lastname: Du
authors.forename: Chuangbin
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authors.lastname: Chen
authors.forename: Shenyan
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authors.lastname: Zhang
authors.forename: Fengji
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authors.lastname: Tan
authors.forename: Youqi
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authors.lastname: Xian
authors.forename: Zhibo
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authors.lastname: Gao
authors.forename: Minghui
authors.initials: M
authors.lastname: He
authors.forename: Longyun
authors.initials: L
authors.lastname: Chen
authors.forename: Xiaohang
authors.initials: X
authors.lastname: Zhao
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
authors.forename: Yunping
authors.initials: Y
authors.lastname: Zhu
authors.forename: Xingfeng
authors.initials: X
authors.lastname: Yin
authors.forename: Huali
authors.initials: H
authors.lastname: Shen
authors.forename: Yang
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authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Jiang
authors.forename: Chengpu
authors.initials: C
authors.lastname: Zhang
authors.forename: Liwei
authors.initials: L
authors.lastname: Li
authors.forename: Cheng
authors.initials: C
authors.lastname: Chang
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.forename: Guoquan
authors.initials: G
authors.lastname: Yan
authors.forename: Jun
authors.initials: J
authors.lastname: Yao
authors.forename: Haojie
authors.initials: H
authors.lastname: Lu
authors.forename: Wantao
authors.initials: W
authors.lastname: Ying
authors.forename: Fan
authors.initials: F
authors.lastname: Zhong
authors.forename: Qing-Yu
authors.initials: QY
authors.lastname: He
authors.forename: Siqi
authors.initials: S
authors.lastname: Liu
mesh_terms: D045744:Cell Line, Tumor; D002890:Chromosomes, Human, Pair 20 / Q000235:genetics / Q000378:metabolism; D003106:Colon / Q000378:metabolism / Q000473:pathology; D005753:Gastric Mucosa / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D015894:Genome, Human; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology; D013058:Mass Spectrometry; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013270:Stomach / Q000473:pathology
chemical_list: D011506:Proteins; D020543:Proteome; D012333:RNA, Messenger