title: ¹H, ¹³C and ¹⁵N resonance assignments of S114A mutant of UVI31+ from Chlamydomonas reinhardtii.
abstract: Almost complete sequence specific (1)H, (13)C and (15)N resonance assignments of S114A mutant of UVI31+ from Chlamydomonas reinhardtii are reported. The cDNA of S114A mutant of UVI31+ was cloned from a eukaryotic green algae (C. reinhardtii) and overexpressed in E.coli from where the protein was purified to homogeneity. The point mutation S114A in UVI31+ reduces its DNA endonuclease activity substantially as compared with its wild type. As a prelude to the structural characterization of S114A mutant of UVI31+, we report here complete sequence-specific (1)H, (13)C and (15)N NMR assignments.
authors.affiliation: Department of Chemical Sciences, Tata Institute of Fundamental Research, 1, Homi Bhabha Road, Colaba, Mumbai, 400005, India.
authors.forename: Himanshu
authors.initials: H
authors.lastname: Singh
authors.forename: Vandana
authors.initials: V
authors.lastname: Raghavan
authors.forename: Manish
authors.initials: M
authors.lastname: Shukla
authors.forename: Basuthkar J
authors.initials: BJ
authors.lastname: Rao
authors.forename: Kandala V R
authors.initials: KV
authors.lastname: Chary
mesh_terms: D002247:Carbon Isotopes; D016825:Chlamydomonas reinhardtii / Q000378:metabolism*; D006859:Hydrogen; D006863:Hydrogen-Ion Concentration; D050505:Mutant Proteins / Q000737:chemistry*; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D010940:Plant Proteins / Q000737:chemistry*; D017433:Protein Structure, Secondary
chemical_list: D002247:Carbon Isotopes; D050505:Mutant Proteins; D009587:Nitrogen Isotopes; D010940:Plant Proteins; D006859:Hydrogen