title: The chromatin remodeler p400 ATPase facilitates Rad51-mediated repair of DNA double-strand breaks.
abstract: DNA damage signaling and repair take place in a chromatin context. Consequently, chromatin-modifying enzymes, including adenosine triphosphate-dependent chromatin remodeling enzymes, play an important role in the management of DNA double-strand breaks (DSBs). Here, we show that the p400 ATPase is required for DNA repair by homologous recombination (HR). Indeed, although p400 is not required for DNA damage signaling, DNA DSB repair is defective in the absence of p400. We demonstrate that p400 is important for HR-dependent processes, such as recruitment of Rad51 to DSB (a key component of HR), homology-directed repair, and survival after DNA damage. Strikingly, p400 and Rad51 are present in the same complex and both favor chromatin remodeling around DSBs. Altogether, our data provide a direct molecular link between Rad51 and a chromatin remodeling enzyme involved in chromatin decompaction around DNA DSBs.
authors.affiliation: Laboratoire de Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération, UMR 5088, Université de Toulouse and 2 Centre National de la Recherche Scientifique, Université Paul Sabatier, 31062 Toulouse, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Courilleau
authors.forename: Catherine
authors.initials: C
authors.lastname: Chailleux
authors.forename: Alain
authors.initials: A
authors.lastname: Jauneau
authors.forename: Fanny
authors.initials: F
authors.lastname: Grimal
authors.forename: Sébastien
authors.initials: S
authors.lastname: Briois
authors.forename: Elisa
authors.initials: E
authors.lastname: Boutet-Robinet
authors.forename: François
authors.initials: F
authors.lastname: Boudsocq
authors.forename: Didier
authors.initials: D
authors.lastname: Trouche
authors.forename: Yvan
authors.initials: Y
authors.lastname: Canitrot
mesh_terms: D002453:Cell Cycle; D002460:Cell Line; D042002:Chromatin Assembly and Disassembly; D053903:DNA Breaks, Double-Stranded; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D006657:Histones / Q000378:metabolism; D006801:Humans; D010766:Phosphorylation; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D021381:Protein Transport; D034622:RNA Interference; D051135:Rad51 Recombinase / Q000378:metabolism*; D059767:Recombinational DNA Repair; D051716:Replication Protein A / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D004268:DNA-Binding Proteins; D006657:Histones; C497167:RPA1 protein, human; D051716:Replication Protein A; C495153:RAD51 protein, human; D051135:Rad51 Recombinase; D004265:DNA Helicases; C498558:EP400 protein, human