title: Native ion mobility-mass spectrometry and related methods in structural biology.
abstract: Mass spectrometry-based methods have become increasingly important in structural biology - in particular for large and dynamic, even heterogeneous assemblies of biomolecules. Native electrospray ionization coupled to ion mobility-mass spectrometry provides access to stoichiometry, size and architecture of noncovalent assemblies; while non-native approaches such as covalent labeling and H/D exchange can highlight dynamic details of protein structures and capture intermediate states. In this overview article we will describe these methods and highlight some recent applications for proteins and protein complexes, with particular emphasis on native MS analysis. This article is part of a Special Issue entitled: Mass spectrometry in structural biology.
authors.affiliation: Department of Chemistry, University of Antwerp, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Konijnenberg
authors.forename: A
authors.initials: A
authors.lastname: Butterer
authors.forename: F
authors.initials: F
authors.lastname: Sobott
mesh_terms: D007477:Ions / Q000737:chemistry; D056004:Molecular Dynamics Simulation; D011487:Protein Conformation; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D007477:Ions; D011506:Proteins