title: High-speed interferometric spectrally encoded flow cytometry.
abstract: Spectrally encoded flow cytometry (SEFC) is a promising technique for noninvasive in vivo microscopy of blood cells. Here, we introduce a novel SEFC system for label-free confocal imaging of blood cells flowing at velocities of up to 10  mm/s within 65 μm-diameter vessels. The new system employs interferometric Fourier-domain detection and a high-speed wavelength-swept source, allowing 100 kHz line rate, sufficient for sampling the rapidly flowing cells 80 μm below the tissue surface. The large data sets obtained by this technique would improve diagnosis accuracy, reduce imaging time, and open new possibilities for noninvasive monitoring of blood in patients.
authors.affiliation: Faculty of Biomedical Engineering, Technion—Israel Institute of Technology, Haifa 32000, Israel.
authors.forename: Lior
authors.initials: L
authors.lastname: Golan
authors.forename: Daniella
authors.initials: D
authors.lastname: Yeheskely-Hayon
authors.forename: Limor
authors.initials: L
authors.lastname: Minai
authors.forename: Dvir
authors.initials: D
authors.lastname: Yelin
mesh_terms: D002469:Cell Separation / Q000295:instrumentation*; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004912:Erythrocytes / Q000166:cytology* / Q000502:physiology*; D005434:Flow Cytometry / Q000295:instrumentation*; D006801:Humans; D007368:Interferometry / Q000295:instrumentation*; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*; D013057:Spectrum Analysis / Q000295:instrumentation*