title: Stability of AMH measurement in blood and avoidance of proteolytic changes.
abstract: The new Gen II assay for anti-Müllerian hormone (AMH) shows good stability and reliability in serum, but analyses of stability in whole blood are lacking. Testing the effects of storage of whole-blood samples at room temperature revealed significant increases in the measured value of AMH of 31% over 4 days (P<0.001). The effect is temperature dependent, with storage at 4°C showing markedly reduced increments. Further, samples collected into serum tubes with gel separators and centrifuged within 5h (blood cells and serum physically separated within the collection tube) showed reliable stability over a period of more than 5 days.
authors.affiliation: Glasgow Centre for Reproductive Medicine, 21 Fifty Pitches Way, Glasgow G51 4FD, UK. richard.fleming@gcrm.co.uk
authors.forename: Richard
authors.initials: R
authors.lastname: Fleming
authors.forename: Craig
authors.initials: C
authors.lastname: Fairbairn
authors.forename: Catherine
authors.initials: C
authors.lastname: Blaney
authors.forename: Dorothy
authors.initials: D
authors.lastname: Lucas
authors.forename: Marco
authors.initials: M
authors.lastname: Gaudoin
mesh_terms: D054304:Anti-Mullerian Hormone / Q000097:blood*; D001774:Blood Chemical Analysis / Q000379:methods*; D001800:Blood Specimen Collection; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D055550:Protein Stability; D059748:Proteolysis; D013696:Temperature; D013997:Time Factors
chemical_list: D054304:Anti-Mullerian Hormone