title: Effect of human whole saliva on the in vitro adhesion of Candida albicans to a denture base acrylic resin: a focus on collection and preparation of saliva samples.
abstract: OBJECTIVE
  In studies on Candida albicans adhesion to surfaces, diverse protocols have been used for collection and preparation of saliva samples. Thus, this study investigated whether variations in the centrifugation parameters and number of donors of saliva would influence the adhesion of C. albicans to a denture base resin.
  
  
  METHODS
  Resin acrylic samples (n = 72) were made and then divided into four groups: (a) control - specimens were left without preconditioning in saliva; (b) three experimental groups, in which the specimens were preconditioned with saliva collected from 15 volunteers and centrifuged at 12 000 g for 5 min (G1 ); from 15 volunteers and centrifuged at 18 000 g for 30 min (G2 ); and from one volunteer and centrifuged at 12 000 g for 5 min (G3 ). Candida adhesion was evaluated by both the 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) reduction method and crystal violet staining. Data were analyzed by one-way analyses of variance (P = 0.05).
  
  
  RESULTS
  For XTT reduction assay, groups G2 , G3 , and control were not significantly different, whereas group G1 showed significantly higher absorbance value than control. For crystal violet staining there were no significant differences among all groups.
  
  
  CONCLUSIONS
  Variations in the centrifugation parameters and number of donors of saliva may influence C. albicans adhesion to denture base resins.
authors.affiliation: Department of Dental Materials and Prosthodontics, Araraquara Dental School, São Paulo State University - UNESP, São Paulo, Brazil.
authors.forename: Camila A
authors.initials: CA
authors.lastname: Zamperini
authors.forename: Patricia C S
authors.initials: PC
authors.lastname: Schiavinato
authors.forename: Ana C
authors.initials: AC
authors.lastname: Pavarina
authors.forename: Eunice T
authors.initials: ET
authors.lastname: Giampaolo
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Vergani
authors.forename: Ana L
authors.initials: AL
authors.lastname: Machado
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000268:Adhesiveness; D002176:Candida albicans / Q000502:physiology*; D015169:Colony Count, Microbial; D004396:Coloring Agents; D003764:Dental Materials / Q000737:chemistry*; D044622:Dental Pellicle / Q000502:physiology; D003819:Denture Bases / Q000382:microbiology*; D005840:Gentian Violet; D006801:Humans; D007202:Indicators and Reagents; D012463:Saliva / Q000502:physiology*; D013048:Specimen Handling / Q000379:methods; D013778:Tetrazolium Salts; D013997:Time Factors
chemical_list: D000180:Acrylic Resins; D004396:Coloring Agents; D003764:Dental Materials; D007202:Indicators and Reagents; D013778:Tetrazolium Salts; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide; D005840:Gentian Violet