title: Diversification of oral and aboral mesodermal regulatory states in pregastrular sea urchin embryos.
abstract: Specification of the non-skeletogenic mesoderm (NSM) in sea urchin embryos depends on Delta signaling. Signal reception leads to expression of regulatory genes that later contribute to the aboral NSM regulatory state. In oral NSM, this is replaced by a distinct oral regulatory state in consequence of Nodal signaling. Through regulome wide analysis we identify the homeobox gene not as an immediate Nodal target. not expression in NSM causes extinction of the aboral regulatory state in the oral NSM, and expression of a new suite of regulatory genes. All NSM specific regulatory genes are henceforth expressed exclusively, in oral or aboral domains, presaging the mesodermal cell types that will emerge. We have analyzed the regulatory linkages within the aboral NSM gene regulatory network. A linchpin of this network is gataE which as we show is a direct Gcm target and part of a feedback loop locking down the aboral regulatory state.
authors.affiliation: California Institute of Technology, Division of Biology, m/c 156-29, Pasadena, CA 91125, USA.
authors.forename: Stefan C
authors.initials: SC
authors.lastname: Materna
authors.forename: Andrew
authors.initials: A
authors.lastname: Ransick
authors.forename: Enhu
authors.initials: E
authors.lastname: Li
authors.forename: Eric H
authors.initials: EH
authors.lastname: Davidson
mesh_terms: D000818:Animals; D046149:Embryo Culture Techniques; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D050980:GATA Transcription Factors / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D053263:Gene Regulatory Networks; D008648:Mesoderm / Q000196:embryology*; D060172:Morpholinos / Q000235:genetics; D009055:Mouth / Q000196:embryology; D055457:Nodal Protein / Q000235:genetics / Q000378:metabolism; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2 / Q000235:genetics / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000235:genetics; D015398:Signal Transduction; D047270:Strongylocentrotus purpuratus / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D050980:GATA Transcription Factors; D060172:Morpholinos; D055457:Nodal Protein; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2; D016376:Oligonucleotides, Antisense; D014157:Transcription Factors