title: Fluorescent protein-based biosensors and their clinical applications.
abstract: Green fluorescent protein and its relatives have shed their light on a wide range of biological problems. To date, with a color palette consisting of fluorescent proteins with different spectra, researchers can "paint" living cells as they desire. Moreover, sophisticated biosensors engineered to contain single or multiple fluorescent proteins, including FRET-based biosensors, spatiotemporally unveil molecular mechanisms underlying physiological processes. Although such molecules have contributed considerably to basic research, their abilities to be used in applied life sciences have yet to be fully explored. Here, we review the molecular bases of fluorescent proteins and fluorescent protein-based biosensors and focus on approaches aimed at applying such proteins to the clinic.
authors.affiliation: Laboratory of Pathophysiology and Signal Transduction, Hokkaido University Graduate School of Medicine, N15W7, Kita-ku, Sapporo, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ohba
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Fujioka
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Nakada
authors.forename: Masumi
authors.initials: M
authors.lastname: Tsuda
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry; D015374:Biosensing Techniques; D019008:Drug Resistance, Neoplasm; D005434:Flow Cytometry / Q000379:methods; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006801:Humans; D007477:Ions; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism; D008958:Models, Molecular; D013050:Spectrometry, Fluorescence / Q000379:methods; D014443:Tyrosine / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; C485182:Cyan Fluorescent Protein; D005456:Fluorescent Dyes; D007477:Ions; D049452:Green Fluorescent Proteins; D014443:Tyrosine