title: Visualization of spinal afferent innervation in the mouse colon by AAV8-mediated GFP expression.
abstract: BACKGROUND
  Primary afferent neurons whose cell bodies reside in thoracolumbar and lumbosacral dorsal root ganglia (DRG) innervate colon and transmit sensory signals from colon to spinal cord under normal conditions and conditions of visceral hypersensitivity. Histologically, these extrinsic afferents cannot be differentiated from intrinsic fibers of enteric neurons because all known markers label neurons of both populations. Adeno-associated virus (AAV) vectors are capable of transducing DRG neurons after intrathecal administration. We hypothesized that AAV-driven overexpression of green fluorescent protein (GFP) in DRG would enable visualization of extrinsic spinal afferents in colon separately from enteric neurons.
  
  
  METHODS
  Recombinant AAV serotype 8 (rAAV8) vector carrying the GFP gene was delivered via direct lumbar puncture. Green fluorescent protein labeling in DRG and colon was examined using immunohistochemistry.
  
  
  RESULTS
  Analysis of colon from rAAV8-GFP-treated mice demonstrated GFP-immunoreactivity (GFP-ir) within mesenteric nerves, smooth muscle layers, myenteric plexus, submucosa, and mucosa, but not in cell bodies of enteric neurons. Notably, GFP-ir colocalized with CGRP and TRPV1 in mucosa, myenteric plexus, and globular-like clusters surrounding nuclei within myenteric ganglia. In addition, GFP-positive fibers were observed in close association with blood vessels of mucosa and submucosa. Analysis of GFP-ir in thoracolumbar and lumbosacral DRG revealed that levels of expression in colon and L6 DRG appeared to be related.
  
  
  CONCLUSIONS
  These results demonstrate the feasibility of gene transfer to mouse colonic spinal sensory neurons using intrathecal delivery of AAV vectors and the utility of this approach for histological analysis of spinal afferent nerve fibers within colon.
authors.affiliation: Department of Neuroscience University of Minnesota, Minneapolis, MN, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Schuster
authors.forename: J A
authors.initials: JA
authors.lastname: Dykstra
authors.forename: M S
authors.initials: MS
authors.lastname: Riedl
authors.forename: K F
authors.initials: KF
authors.lastname: Kitto
authors.forename: C N
authors.initials: CN
authors.lastname: Honda
authors.forename: R S
authors.initials: RS
authors.lastname: McIvor
authors.forename: C A
authors.initials: CA
authors.lastname: Fairbanks
authors.forename: L
authors.initials: L
authors.lastname: Vulchanova
mesh_terms: D000818:Animals; D003106:Colon / Q000294:innervation*; D000229:Dependovirus / Q000235:genetics; D005727:Ganglia, Spinal; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D007150:Immunohistochemistry; D051379:Mice; D009197:Myenteric Plexus; D009475:Neurons, Afferent / Q000166:cytology*; D014161:Transduction, Genetic / Q000379:methods
chemical_list: D049452:Green Fluorescent Proteins