title: UPLC-Q-TOF/HSMS/MS(E)-based metabonomics for adenine-induced changes in metabolic profiles of rat faeces and intervention effects of ergosta-4,6,8(14),22-tetraen-3-one.
abstract: Ergosta-4,6,8(14),22-tetraen-3-one (ergone), isolated from the medicinal fungus Polyporus umbellatus, has been proven to prevent the progression of renal injury and the subsequent renal fibrosis. Ultra performance liquid chromatography coupled with quadrupole time-of-flight high-sensitivity mass spectrometry and a novel mass spectrometry(Elevated Energy) (MS(E)) data collection technique was employed to investigate metabonomic characters of chronic renal failure (CRF) induced adenine and the protective effects of ergosta-4,6,8(14),22-tetraen-3-one (ergone). Coupled with blood biochemistry and kidney histopathology results, the significant difference in metabolic profiling between adenine-induced CRF group and ergone-treated CRF group by using pattern recognition analysis indicated that changes in global faecal metabolites were occurred. Seven endogenous metabolites were identified by using metabonomic method combined with multivariate data analysis, the accurate mass, isotopic pattern, MS(E) fragments information and MassLynx i-FIT algorithm. These biochemical changes in faecal metabolites are related to the perturbations of bile acid metabolism and phospholipid metabolism, which may be helpful to further understand the CRF and therapeutic mechanisms of ergone. This research proved that MS(E) can simultaneous acquire precursor ion information and fragment ion data at high and low collision energy in one analytical run, which facilitated the fast structural characterization of metabolites.
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, the College of Life Sciences, Northwest University, No. 229 Taibai North Road, Xi'an, Shaanxi 710069, PR China. zyy@nwu.edu.cn
authors.forename: Ying-Yong
authors.initials: YY
authors.lastname: Zhao
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Feng-Ya
authors.initials: FY
authors.lastname: Long
authors.forename: Xian-Long
authors.initials: XL
authors.lastname: Cheng
authors.forename: Xu
authors.initials: X
authors.lastname: Bai
authors.forename: Feng
authors.initials: F
authors.lastname: Wei
authors.forename: Rui-Chao
authors.initials: RC
authors.lastname: Lin
mesh_terms: D000225:Adenine; D000818:Animals; D002783:Cholestenones / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016002:Discriminant Analysis; D005243:Feces / Q000737:chemistry; D006651:Histocytochemistry; D007676:Kidney Failure, Chronic / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D013058:Mass Spectrometry / Q000379:methods; D055432:Metabolomics / Q000379:methods; D051381:Rats
chemical_list: D002783:Cholestenones; D000225:Adenine; C015860:ergosta-4,6,8(14),22-tetraen-3-one