title: Hydroxyapatite/regenerated silk fibroin scaffold-enhanced osteoinductivity and osteoconductivity of bone marrow-derived mesenchymal stromal cells.
abstract: A novel hydroxyapatite/regenerated silk fibroin scaffold was prepared and investigated for its potential to enhance both osteoinductivity and osteoconductivity of bone marrow-derived mesenchymal stromal cells in vitro. Approx. 12.4 ± 0.06 % (w/w) hydroxyapatite was deposited onto the scaffold, and cell viability and DNA content were significantly increased (18.5 ± 0.6 and 33 ± 1.2 %, respectively) compared with the hydroxyapatite scaffold after 14 days. Furthermore, alkaline phosphatase activity in the novel scaffold increased 41 ± 2.5 % after 14 days compared with the hydroxyapatite scaffold. The data indicate that this novel hydroxyapatite/regenerated silk fibroin scaffold has a positive effect on osteoinductivity and osteoconductivity, and may be useful for bone tissue engineering.
authors.affiliation: Fudan University Sports Medicine Center, Department of Sports Medicine and Arthroscopy Surgery, Huashan Hospital, Fudan University, No. 12 Wulumuqi Zhong Road, Shanghai 200040, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Jiang
authors.forename: Wei
authors.initials: W
authors.lastname: Hao
authors.forename: Yuzhuo
authors.initials: Y
authors.lastname: Li
authors.forename: Jinrong
authors.initials: J
authors.lastname: Yao
authors.forename: Zhengzhong
authors.initials: Z
authors.lastname: Shao
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Jianjun
authors.initials: J
authors.lastname: Yang
authors.forename: Shiyi
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000032:analysis; D017886:Durapatite / Q000378:metabolism*; D005349:Fibroins / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000502:physiology*; D011817:Rabbits; D047011:Silk / Q000378:metabolism*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D047011:Silk; D004247:DNA; D005349:Fibroins; D017886:Durapatite