title: Depletion of IL-22 during culture enhanced antigen-driven IFN-γ production by CD4(+)T cells from patients with active TB.
abstract: In this study, we mainly investigated the expression of IFN-γ and IL-22-producing CD4(+)T cells, the relationship between the two cytokines in human tuberculosis. We showed that IFN-γ and IL-22 were induced by human mycobacterial infection. Besides, CD4(+)T cells expressing IFN-γ and IL-22 were significantly elevated following PPD stimulation. In addition, IFN-γ and IL-22 -expressing CD4(+)T cells were distinct from each other. We also found that there was a reciprocal relationship between IFN-γ and IL-22 production. The production of IL-22 was markedly enhanced in the absence of IFN-γ, also, the expression of IFN-γ was increased when the IL-22 signaling was inhibited by monoclonal anti-IL-22 mAb. Furthermore, data revealed that the detectable IL-22 affected the phynotype of IFN-γ(+)CD4(+)T cells. IL-22 induced up-regulation of CD27 expression in IFN-γ(+)CD4(+)T cells, which might further confirm that IL-22 had an effect on the response of IFN-γ. Thus, this work provides a previous unknown relationship between IFN-γ and IL-22 in human TB disease.
authors.affiliation: Department of Chemistry, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
authors.forename: Yueqin
authors.initials: Y
authors.lastname: Qiu
authors.forename: Yu
authors.initials: Y
authors.lastname: Huang
authors.forename: Jianbo
authors.initials: J
authors.lastname: Chen
authors.forename: Dongjuan
authors.initials: D
authors.lastname: Qiao
authors.forename: Gucheng
authors.initials: G
authors.lastname: Zeng
authors.forename: Jiye
authors.initials: J
authors.lastname: Cai
mesh_terms: D000328:Adult; D000941:Antigens / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D007378:Interleukins / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D015398:Signal Transduction; D014376:Tuberculosis / Q000276:immunology* / Q000378:metabolism*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000378:metabolism; D015854:Up-Regulation; D055815:Young Adult; D000097043:Interleukin-22
chemical_list: D000941:Antigens; D007371:Interferon-gamma; D007378:Interleukins; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D000097043:Interleukin-22