title: Expression of angiogenesis-stimulating factors (VEGF, CD31, CD105) and angiogenetic index in gingivae of patients with chronic periodontitis.
abstract: The aim of this study was to determine the prognostic value of the angiogenesis rate in chronic periodontitis (CP). A total of 61 human gingival samples were taken from patients with CP (n = 40) obtained during open curettage with gingivectomy, healthy periodontia (n = 15), and reactive lymph nodes (n = 7). Quantitative immunocytochemistry studies of VEGF, CD31 (PECAM-1) and CD105 (endoglin) were performed using the spatial visualization method. CD105/CD31 and VEGF/CD31 angiogenetic ratios (ARs) were established to determine the proliferation fraction of the endothelium. In patients with CP, the proliferation of blood vessels was observed, including the presence of numerous high endothelial venules (HEVs) and ordinary vessels. In gingival HEVs of patients with CP, the higher expression was shown by CD31 and, in turn CD105 and VEGF. The entire vascular expression of CD31 in the gingiva correlates with grading in lamina propria, but our study failed to document correlations between the expression of VEGF and CD105 and clinical data of patients with CP. Higher ARs were seen in gingivae of CP patients compared to controls. We concluded that overexpression of the angiogenesis-associated factors in CP suggests its significance in protracting the inflammatory process or periodic exacerbations of the process and destruction of the periodontium. The increased CD105/CD31 and VEGF/CD31 ratios in gingiva confirms an augmented proliferative fraction of the endothelium in gingiva with CP.
authors.affiliation: Department of Histology and Embryology, Poznan University of Medical Sciences in Poznan, Poland. akasprza@ump.edu.pl
authors.forename: Aldona
authors.initials: A
authors.lastname: Kasprzak
authors.forename: Anna
authors.initials: A
authors.lastname: Surdacka
authors.forename: Maciej
authors.initials: M
authors.lastname: Tomczak
authors.forename: Wieslawa
authors.initials: W
authors.lastname: Przybyszewska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Seraszek-Jaros
authors.forename: Agata
authors.initials: A
authors.lastname: Malkowska-Lanzafame
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Siodla
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Kaczmarek
mesh_terms: D000328:Adult; D043925:Angiogenesis Inducing Agents / Q000378:metabolism*; D015703:Antigens, CD / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D055113:Chronic Periodontitis / Q000378:metabolism / Q000473:pathology*; D000071063:Endoglin; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D005881:Gingiva / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D007249:Inflammation / Q000473:pathology; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D008297:Male; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D014699:Venules / Q000473:pathology
chemical_list: D043925:Angiogenesis Inducing Agents; D015703:Antigens, CD; D015415:Biomarkers; C064206:ENG protein, human; D000071063:Endoglin; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011956:Receptors, Cell Surface; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A