title: Melatonin is involved in the apoptosis and necrosis of pancreatic cancer cell line SW-1990 via modulating of Bcl-2/Bax balance.
abstract: Melatonin influences a number of physiological processes and is believed to play an antitumoral role in several types of cancers, but its impact on pancreatic cancer is not fully clarified. The growth inhibitory effect of melatonin on pancreatic cancer cell line SW-1990 was detected in vitro and in vivo. Annexin V/PI assay was applied to detect apoptosis and necrosis in SW-1990 cells. Changes of Bcl-2 and Bax expression were investigated by RT-PCR and Western blot. An obvious growth inhibition was found in SW-1990 after melatonin or combined treatment with melatonin and gemicitabine through both apoptosis and necrosis in vitro, and also found in transplanted tumors in nude mice. RT-PCR and Western blot showed that Bcl-2 expression was downregulated, while Bax expression was upregulated, after melatonin treatment. Melatonin may be a pro-apoptotic and pro-necrotic agent for pancreatic cancer cells via its modulation of Bcl-2/Bax balance.
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Canglang District, Suzhou 215006, Jiangsu Province, PR China.
authors.forename: Chunfang
authors.initials: C
authors.lastname: Xu
authors.forename: Airong
authors.initials: A
authors.lastname: Wu
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.forename: Huaying
authors.initials: H
authors.lastname: Fang
authors.forename: Lele
authors.initials: L
authors.lastname: Xu
authors.forename: Jianxin
authors.initials: J
authors.lastname: Ye
authors.forename: Jiaqing
authors.initials: J
authors.lastname: Shen
mesh_terms: D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003841:Deoxycytidine / Q000008:administration & dosage / Q000031:analogs & derivatives; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000235:genetics; D006801:Humans; D008550:Melatonin / Q000008:administration & dosage; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009336:Necrosis / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism*; D000093542:Gemcitabine
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D003841:Deoxycytidine; D008550:Melatonin; D000093542:Gemcitabine