title: Growth of v-src-transformed cells in serum-free medium through the induction of growth factors.
abstract: The v-src oncogene is one of only two oncogenes capable of transforming mouse embryo fibroblasts (MEFs) lacking the IGF-IR gene (R-cells). R-/v-src cells grow robustly in the absence of serum, suggesting the hypothesis that they may produce one or more growth factors that would sustain their ability to proliferate in serum-free condition. Using proteomic approaches on serum-free conditioned media derived from v-src-transformed cells, we have identified two growth promoting factors: ostepontin and proliferin. Subsequent experiments have indicated that osteopontin plays a prevalent role in promoting growth of v-src-transformed cells in serum-deprived condition.
authors.affiliation: Department of Cancer Biology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania, USA.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Deangelis
authors.forename: Andrew
authors.initials: A
authors.lastname: Quong
authors.forename: Andrea
authors.initials: A
authors.lastname: Morrione
authors.forename: Renato
authors.initials: R
authors.lastname: Baserga
mesh_terms: D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000378:metabolism*; D016895:Culture Media, Serum-Free; D055785:Gene Knockdown Techniques; D016391:Genes, src; D006023:Glycoproteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D053495:Osteopontin / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D011388:Prolactin; D040901:Proteomics; D017526:Receptor, IGF Type 1 / Q000172:deficiency / Q000235:genetics; D015398:Signal Transduction
chemical_list: D016895:Culture Media, Serum-Free; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; C000634106:Prl2c2 protein, mouse; C506896:Spp1 protein, mouse; D053495:Osteopontin; D011388:Prolactin; D017526:Receptor, IGF Type 1