title: Small hepatitis B surface antigen interacts with and modulates enoyl-coenzyme A hydratase expression in hepatoma cells.
abstract: Enoyl-coenzyme A hydratase (ECHS1) is a key enzyme in the metabolism of fatty acids in mitochondria. We previously reported that hepatitis B surface antigen (HBsAg) interacted with ECHS1 in a yeast two-hybrid system. In the current study, we further examined their interaction by using GST pull-down and co-immunoprecipitation assays. The results confirmed that small hepatitis B surface antigen (SHBs) interacted with ECHS1. Furthermore, confocal imaging showed that SHBs and ECHS1 co-localized in HepG2 cells. To clarify the biological function of the interaction, human hepatoma cell lines that transiently and stably expressed SHBs were generated. The expression of SHBs led to a significant decrease in ECHS1 protein levels. ECHS1 protein levels were reduced to 48.44 ± 7.12 % in Huh7 cells transiently expressing SHBs, and to 54.97 ± 3.54 % in HepG2 cells stably expressing SHBs. In conclusion, our findings suggest that SHBs interacts with ECHS1 and regulates ECHS1 protein levels in hepatoma cells.
authors.affiliation: Center of Liver Diseases, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, Fujian, China.
authors.forename: Xianqiong
authors.initials: X
authors.lastname: Gong
authors.forename: Yueyong
authors.initials: Y
authors.lastname: Zhu
authors.forename: Jing
authors.initials: J
authors.lastname: Dong
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Jia
authors.initials: J
authors.lastname: You
authors.forename: Qi
authors.initials: Q
authors.lastname: Zheng
authors.forename: Zilan
authors.initials: Z
authors.lastname: Rao
authors.forename: Qianguo
authors.initials: Q
authors.lastname: Mao
authors.forename: Jiaji
authors.initials: J
authors.lastname: Jiang
mesh_terms: D002460:Cell Line; D002498:Centrifugation; D004746:Enoyl-CoA Hydratase / Q000378:metabolism*; D006514:Hepatitis B Surface Antigens / Q000378:metabolism*; D006515:Hepatitis B virus / Q000472:pathogenicity*; D022781:Hepatocytes / Q000821:virology*; D054884:Host-Pathogen Interactions; D006801:Humans; D047468:Immunoprecipitation; D018613:Microscopy, Confocal; D025941:Protein Interaction Mapping; D020798:Two-Hybrid System Techniques
chemical_list: D006514:Hepatitis B Surface Antigens; D004746:Enoyl-CoA Hydratase