title: Single-molecule imaging in live bacteria cells.
abstract: Bacteria, such as Escherichia coli and Caulobacter crescentus, are the most studied and perhaps best-understood organisms in biology. The advances in understanding of living systems gained from these organisms are immense. Application of single-molecule techniques in bacteria have presented unique difficulties owing to their small size and highly curved form. The aim of this review is to show advances made in single-molecule imaging in bacteria over the past 10 years, and to look to the future where the combination of implementing such high-precision techniques in well-characterized and controllable model systems such as E. coli could lead to a greater understanding of fundamental biological questions inaccessible through classic ensemble methods.
authors.affiliation: Department of Physics, Purdue University, West Lafayette, IN 47907, USA. kpritchie@purdue.edu
authors.forename: Ken
authors.initials: K
authors.lastname: Ritchie
authors.forename: Yoriko
authors.initials: Y
authors.lastname: Lill
authors.forename: Chetan
authors.initials: C
authors.lastname: Sood
authors.forename: Hochan
authors.initials: H
authors.lastname: Lee
authors.forename: Shunyuan
authors.initials: S
authors.lastname: Zhang
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry; D002462:Cell Membrane / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry*; D004058:Diffusion; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D015964:Gene Expression Regulation, Bacterial; D008565:Membrane Proteins / Q000737:chemistry; D026901:Membrane Transport Proteins / Q000737:chemistry; D057054:Molecular Imaging / Q000379:methods*; D036103:Nanotechnology; D021381:Protein Transport; D012680:Sensitivity and Specificity; D013194:Staining and Labeling
chemical_list: D001425:Bacterial Outer Membrane Proteins; C044592:BtuB protein, E coli; D029968:Escherichia coli Proteins; D005456:Fluorescent Dyes; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C043426:inner membrane protein, E coli