title: Highly coordinated proteome dynamics during reprogramming of somatic cells to pluripotency.
abstract: Generation of induced pluripotent stem cells (iPSCs) is a process whose mechanistic underpinnings are only beginning to emerge. Here, we applied in-depth quantitative proteomics to monitor proteome changes during the course of reprogramming of fibroblasts to iPSCs. We uncover a two-step resetting of the proteome during the first and last 3 days of reprogramming, with multiple functionally related proteins changing in expression in a highly coordinated fashion. This comprised several biological processes, including changes in the stoichiometry of electron transport-chain complexes, repressed vesicle-mediated transport during the intermediate stage, and an EMT-like process in the late phase. In addition, we demonstrate that the nucleoporin Nup210 is essential for reprogramming by its permitting of rapid cellular proliferation and subsequent progression through MET. Along with the identification of proteins expressed in a stage-specific manner, this study provides a rich resource toward an enhanced mechanistic understanding of cellular reprogramming.
authors.affiliation: European Molecular Biology Laboratory, Genome Biology Unit, Heidelberg, Germany.
authors.forename: Jenny
authors.initials: J
authors.lastname: Hansson
authors.forename: Mahmoud Reza
authors.initials: MR
authors.lastname: Rafiee
authors.forename: Sonja
authors.initials: S
authors.lastname: Reiland
authors.forename: Jose M
authors.initials: JM
authors.lastname: Polo
authors.forename: Julian
authors.initials: J
authors.lastname: Gehring
authors.forename: Satoshi
authors.initials: S
authors.lastname: Okawa
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Huber
authors.forename: Konrad
authors.initials: K
authors.lastname: Hochedlinger
authors.forename: Jeroen
authors.initials: J
authors.lastname: Krijgsveld
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*
chemical_list: D028861:Nuclear Pore Complex Proteins; C512902:Nup210 protein, mouse; D020543:Proteome