title: Effects of nutritional enrichment on the production of acetone-butanol-ethanol (ABE) by Clostridium acetobutylicum.
abstract: Clostridium acetobutylicum is an industrially important organism that produces acetone-butanol-ethanol (ABE). The main objective of this study was to characterize the effects of increased cell density on the production of ABE during the phase transition from acidogenesis to solventogenesis in C. acetobutylicum. The increased ABE productivity of C. acetobutylicum was obtained by increasing the cell density using a newly designed medium (designated C. a                 cetobutylicum                 medium 1; CAM1). The maximum OD(600) value of C. acetobutylicum ATCC 824 strain obtained with CAM1 was 19.7, which is 1.8 times higher than that obtained with clostridial growth medium (CGM). The overall ABE productivity obtained in the CAM1-fermetation of the ATCC 824 strain was 0.83 g/L/h, which is 1.5 times higher than that (0.55 g/L/h) obtained with CGM. However, the increased productivity obtained with CAM1 did not result in an increase in the final ABE titer, because phase transition occurred at a high titer of acids.
authors.affiliation: Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: Sung Jun
authors.initials: SJ
authors.lastname: Choi
authors.forename: Joungmin
authors.initials: J
authors.lastname: Lee
authors.forename: Yu-Sin
authors.initials: YS
authors.lastname: Jang
authors.forename: Jin Hwan
authors.initials: JH
authors.lastname: Park
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
authors.forename: In Ho
authors.initials: IH
authors.lastname: Kim
mesh_terms: D000096:Acetone / Q000378:metabolism*; D056804:Biofuels; D000440:Butanols / Q000378:metabolism*; D046969:Clostridium acetobutylicum / Q000254:growth & development* / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation
chemical_list: D056804:Biofuels; D000440:Butanols; D000096:Acetone; D000431:Ethanol