title: Antioxidant cosmeto-textiles: skin assessment.
abstract: Resveratrol, a natural product, has been reported to have antioxidant activities such as the scavenging of free radicals. This compound could be used in the dermocosmetic field to protect the skin from oxidative stress. In this work, the percutaneous profile of resveratrol in ethanol solutions through pig skin was determinated by an in vitro methodology. The percutaneous absorption of resveratrol was measured and compared with trolox, an analogous of Vitamin E. Both antioxidants were found in all skin sections (stratum corneum, epidermis, and dermis). Besides, the free radical scavenging activity of resveratrol and trolox has been evaluated using DPPH method. The effective dose (ED₅₀) of compounds and DPPH radical inhibition in each skin layer were evaluated. Under the conditions used for these experiments, it can be deduced that resveratrol is more efficient than trolox as an antioxidant, also in the inner skin layers. The cosmeto-textiles with an active substance incorporated into their structure are increasingly used in the cosmetics and pharmaceutical industries. The action of several cosmeto-textiles on the skin was assessed by in vitro and in vivo methodologies. Samples of these cosmeto-textiles were prepared with resveratrol incorporated into cotton and polyamide fabrics. An in vitro percutaneous absorption was used to demonstrate the delivery of the resveratrol from the textile to the different skin layers (stratum corneum, epidermis, and dermis). Additionally, these cosmeto-textiles containing the antioxidant were applied onto the forearms of volunteers to evaluate the textiles' efficacy in skin penetration. The antioxidant's antiradical capacity was evaluated using the DPPH method. Results showed that resveratrol could be detected in the dermis, epidermis, and stratum corneum (SC) by an in vitro percutaneous absorption method and was also detected in the outermost layers of the SC by an in vivo method (stripping). A smaller amount of resveratrol was penetrated through the skin layers when cosmeto-textiles were used compared to direct topical application of the antioxidant solution. The cosmeto-textiles investigated can act as a reservoir system capable of progressively deliver the active substance to the skin layers. From the skin penetration profiles and the antioxidant efficacy assessment of resveratrol, it is possible to ameliorate the inherent antioxidant capacity of skin.
authors.affiliation: Advanced Chemical Institute of Catalonia (IQAC-CSIC), Barcelona, Spain. cristina.alonso@iqac.csic.es
authors.forename: Cristina
authors.initials: C
authors.lastname: Alonso
authors.forename: Meritxell
authors.initials: M
authors.lastname: Martí
authors.forename: Vanessa
authors.initials: V
authors.lastname: Martínez
authors.forename: Laia
authors.initials: L
authors.lastname: Rubio
authors.forename: José L
authors.initials: JL
authors.lastname: Parra
authors.forename: Luisa
authors.initials: L
authors.lastname: Coderch
mesh_terms: D000328:Adult; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000493:pharmacokinetics; D002839:Chromans / Q000008:administration & dosage / Q000493:pharmacokinetics; D003358:Cosmetics / Q000008:administration & dosage* / Q000493:pharmacokinetics; D005260:Female; D006801:Humans; D009924:Organ Culture Techniques; D000077185:Resveratrol; D012867:Skin / Q000187:drug effects / Q000378:metabolism; D012869:Skin Absorption / Q000187:drug effects / Q000502:physiology*; D013267:Stilbenes / Q000008:administration & dosage* / Q000493:pharmacokinetics; D013552:Swine; D013784:Textiles
chemical_list: D000975:Antioxidants; D002839:Chromans; D003358:Cosmetics; D013267:Stilbenes; D000077185:Resveratrol; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid