title: [Establishment of anther culture system for Angelica dahurica from Sichuan province].
abstract: OBJECTIVE
  To research the optimal conditions for the callus induction of anther culture and the plant regeneration of Angelica dahurica var. formosana.
  
  
  METHODS
  Callus was induced from the anther of A. dahurica from Sichuan province on a MS medium. The effects of callus induction and plant regeneration of different pretreatment hours under low temperature (4 degrees C), different culturing conditions under darkness and illumination, and different culture with different hormone contents and ratios were studied.
  
  
  RESULTS
  The results showed that A. dahurica anthers without low temperature pretreatment reached the highest induction rate then under the pretreatment under low temperature (4 degrees C) for two days. The optimal culturing condition was under the darkness. The culturing efficiency reached 38.89% on the medium of MS + 2.0 mg x L(-1) 2,4-D + 1.0 mg x L(-1) 6-BA. The optimum medium for differentiate anther callus was MS + 0.5 mg x L(-1) NAA + 1.5 mg x L(-1) KT + 10 mg x L(-1) AgNO3. 1/2MS medium supplemented with 0.5 mg x L(-1) IBA could well promote seedings to take roots.
  
  
  CONCLUSIONS
  An efficient system for callus induction of anther culture and plant regeneration of A. dahurica was preliminarily established.
authors.affiliation: Agronomy College, Chengdu Campus, Sichuan Agricultural University, Wenjiang 611130, China.
authors.forename: Jing-ye
authors.initials: JY
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Kai
authors.initials: K
authors.lastname: Hou
authors.forename: Yu-ting
authors.initials: YT
authors.lastname: Zou
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: He
mesh_terms: D029969:Angelica / Q000187:drug effects / Q000254:growth & development; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D035264:Flowers; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D003470:Culture Media