title: Stacking and simultaneous determination of estrogens in water samples by CE with electrochemical detection.
abstract: A rapid and sensitive method based on transient ITP and field enhancement in CE with electrochemical detection at copper disk electrode was developed for the simultaneous separation and determination of three estrogens: estrone, 17β-estradiol, and estriol. The effects of several important factors that influence the separation and detection were investigated. Under the optimum conditions, the estrogens could be separated in 0.06 mol/L sodium hydroxide solution within 14 min. With transient ITP by addition of 0.5% NaCl, a good linear response was obtained for three estrogens from 0.2 to 10 μmol/L, with correlation coefficients higher than 0.9993. The detection limits were 8.9 × 10(-8) , 6.7 × 10(-8) , and 1.1 × 10(-7)  mol/L (S/N = 3) for estriol, 17β-estradiol, and estrone, respectively. This method was successfully employed to analyze different water samples from waterworks, tap water, fishpond, and river samples with recoveries in the range of 90.8-108.9%, and RSDs < 4.69%. The satisfied results demonstrated that this method was of convenient preparation, high sensitivity, and good repeatability, which could be applied to the rapid determination of environmental water samples.
authors.affiliation: Department of Environmental Science and Shanghai Key Laboratory for Urban Ecology and Sustainability, East China Normal University, Shanghai, P R China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zhao
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Wang
authors.forename: Guoyue
authors.initials: G
authors.lastname: Shi
authors.forename: Tianshu
authors.initials: T
authors.lastname: Zhou
mesh_terms: D055664:Electrochemical Techniques / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D004967:Estrogens / Q000737:chemistry*; D045483:Rivers / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000737:chemistry*
chemical_list: D004967:Estrogens; D014874:Water Pollutants, Chemical