title: Exploration of the key microbes involved in the cellulolytic activity of a microbial consortium by serial dilution.
abstract: A highly cellulolytic consortium was serially diluted and incubated in fermentation medium with filter paper as carbon sources. The critical dilution point of the consortium for effective degradation of filter paper was 10(-5) as further dilution resulted in the loss of degradation ability of filter paper accompanied by the disappearance of four bands in denaturing gradient gel electrophoresis profiles. The loss of bands corresponding to 16S rRNA sequences from Clostridium clariflavum DSM 19732 and a Paenibacillus species coincided with the loss of degradation ability of filter paper.
authors.affiliation: College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang 330045, PR China. zqh_net@163.com
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Tian
authors.forename: Lei
authors.initials: L
authors.lastname: Tang
authors.forename: Huixing
authors.initials: H
authors.lastname: Li
authors.forename: Wencheng
authors.initials: W
authors.lastname: Li
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhang
authors.forename: Hongjian
authors.initials: H
authors.lastname: Zhang
authors.forename: Zhonggui
authors.initials: Z
authors.lastname: Mao
mesh_terms: D001483:Base Sequence; D057919:Biological Oxygen Demand Analysis; D003013:Clostridium / Q000235:genetics / Q000378:metabolism*; D016000:Cluster Analysis; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D005285:Fermentation; D008031:Lignin / Q000378:metabolism*; D002365:Manihot / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D056507:Paenibacillus / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D012336:RNA, Ribosomal, 16S; C036909:lignocellulose; D008031:Lignin