title: A proteomics approach to detect tissue-wide adaptive changes in the pancreas associated with increased pancreatic α-amylase activity in domestic cattle (Bos taurus).
abstract: We used a proteomics-based approach to investigate potential regulatory proteins in the pancreas of domestic cattle (Bos taurus) that were associated with differences in pancreatic α-amylase activity. Two groups of 48 and 45 crossbred steers in years 1 and 2, respectively, were fed a high moisture corn-based diet and were ranked according to their pancreatic α-amylase activity. Steers (n=18) with high, medium, and low α-amylase activity were selected, with 3 for each activity range and 9 for each experimental year, and their proteomic profiles were compared. Pancreatic samples from each animal were fractionated using 2D-HPLC and fractions detected using UV spectrophotometry. Software analysis revealed 119 common protein fractions among the 18 animals, and statistical analysis revealed 10 of these fractions differing (P<0.10) in abundance between animals from the high and low pancreatic α-amylase activity groups. Five protein fractions identified after tandem mass spectrometry analysis and database searches were found to match proteins with protein-binding, nucleotide/DNA-binding or enzymatic capabilities. Bioinformatics analysis of these fractions revealed porphobilinogen deaminase, a DNA-binding protein, and a putative S1 peptidase that increased in abundance with increasing α-amylase activity; with a putative ATP/GTP binding protein decreasing in abundance with increasing pancreatic α-amylase activity. Changes in these fractions may represent adaptations of the pancreas in domestic cattle that are associated with differences in α-amylase activity.
authors.affiliation: Department of Animal and Poultry Science, University of Guelph, ON, Canada.
authors.forename: Simone
authors.initials: S
authors.lastname: Holligan
authors.forename: Jiaxi
authors.initials: J
authors.lastname: Wang
authors.forename: John P
authors.initials: JP
authors.lastname: Cant
authors.forename: Kendall C
authors.initials: KC
authors.lastname: Swanson
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D030562:Databases, Protein; D004032:Diet; D015180:Electrophoresis, Gel, Two-Dimensional; D008297:Male; D010179:Pancreas / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism; D055292:Pancreatic alpha-Amylases / Q000378:metabolism*; D020543:Proteome / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D020543:Proteome; D055292:Pancreatic alpha-Amylases