title: Biosynthesis and characterization of a new copolymer, poly(3-hydroxyvalerate-co-5-hydroxydecenoate), from Pseudomonas aeruginosa.
abstract: Using a new isolate of Pseudomonas aeruginosa, we obtained 7 g cell dry wt (CDW/l) using 5 % (w/v) glucose. Crude polyhydroxyalkanoates were obtained at 14.6 % of CDW. FTIR and NMR analysis confirmed that this was a new co-polymer: 3-hydroxyvalerate-co-5-hydroxydecenoate. Differential scanning calorimetry analysis showed two different melting temperatures of the copolymer and also indicated the glass transition temperature to be 4 °C. The polydispersity index of the polymer was 1.059.
authors.affiliation: Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, 784028 Sonitpur, Assam, India. pinkeephukon@gmail.com
authors.forename: Pinkee
authors.initials: P
authors.lastname: Phukon
authors.forename: Binod
authors.initials: B
authors.lastname: Pokhrel
authors.forename: B K
authors.initials: BK
authors.lastname: Konwar
authors.forename: S K
authors.initials: SK
authors.lastname: Dolui
mesh_terms: D001704:Biopolymers / Q000737:chemistry* / Q000378:metabolism*; D002151:Calorimetry; D003652:Decanoic Acids / Q000032:analysis*; D005947:Glucose / Q000378:metabolism; D006880:Hydroxy Acids / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D011091:Polyesters / Q000032:analysis*; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D044366:Transition Temperature; D014631:Valerates / Q000032:analysis*
chemical_list: D001704:Biopolymers; D003652:Decanoic Acids; D006880:Hydroxy Acids; D011091:Polyesters; D014631:Valerates; C052853:5-hydroxydecanoic acid; C073314:poly(3-hydroxyvalerate); D005947:Glucose