title: Application of sperm selection using hyaluronic acid binding in intracytoplasmic sperm injection cycles: a sibling oocyte study.
abstract: The purpose of this study was to investigate whether sperm selection by hyaluronic acid (HA) binding could improve fertilization rate and embryo quality in intracytoplasmic sperm injection (ICSI) cycles. Two hundred nineteen oocytes obtained from eighteen women were injected with either HA-bound (n = 107) or conventionally selected spermatozoa (n = 112) in a randomized way. All of the participants were infertile couples who had normal sperm parameters but low fertilization rate in previous in vitro fertilization (IVF) cycle (n = 5) or experienced multiple IVF failures (n = 13). Lower fertilization (75.7% vs 83.0%) and cleavage rate on day 2 (72.9% vs 83.0%) was observed in oocytes injected with HA-bound spermatozoa than the conventional group, but the difference was not significant. Significantly lower cleavage rate was observed on day 3 in HA group (56.0% vs 69.6%, P = 0.038). Blastocyst formation rate and the number of transferred embryos were similar in both groups. In multiple IVF failure patients, significantly reduced fertilization rate (71.8% vs 85.3%, P = 0.046) and cleavage rate on day 2 (70.4% vs 85.3%, P = 0.029) and day 3 (53.5% vs 77.3%, P = 0.002) were noticed in HA group. Five women achieved pregnancy continuing more than 12 weeks after transfer (27.8%). Success of ICSI was not related with the number of embryos fertilized by HA-bound spermatozoa. Application of ICSI by sperm selection using HA binding is not helpful in couples with repeated poor fertilization or implantation despite normal sperm parameters.
authors.affiliation: Institute of Fertility and Genetics, Hamchoon Women's Clinic, Seoul, Korea.
authors.forename: Seung Ah
authors.initials: SA
authors.lastname: Choe
authors.forename: Jin Chul
authors.initials: JC
authors.lastname: Tae
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Shin
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Chung Hyon
authors.initials: CH
authors.lastname: Kim
authors.forename: Joong Yeup
authors.initials: JY
authors.lastname: Lee
authors.forename: Doyeong
authors.initials: D
authors.lastname: Hwang
authors.forename: Ki Chul
authors.initials: KC
authors.lastname: Kim
authors.forename: Chang Suk
authors.initials: CS
authors.lastname: Suh
authors.forename: Byung Chul
authors.initials: BC
authors.lastname: Jee
mesh_terms: D000328:Adult; D001755:Blastocyst / Q000166:cytology; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D007248:Infertility, Male / Q000628:therapy; D008297:Male; D009865:Oocytes / Q000166:cytology / Q000502:physiology; D011247:Pregnancy; D018873:Pregnancy Rate; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology
chemical_list: D006820:Hyaluronic Acid