title: Over-expressing transporters associated with antigen processing increases antitumor immunity response in prostate cancer.
abstract: As we know, prostate cancer down-regulates expression of HLA-1 Antigen Processing Machinery (APM) and has defects in the antigen presentation pathway. In vitro, the prostate cancer cell (PC-3 cells) infected with Lentivirus TAP1 can efficiently over-express TAP1 and Tapasin, and HLA-1 was also up-regulated on the surface of the infected cells. The lentivirus TAP1 infection increased the apoptosis rate of PC-3 cells. In addition, with the co-cluture PC-3 cells and lymphocytes, TAP1 augmented the expression of CD3⁺CD8⁺CD38⁺ T cell. Importantly, administration of Lentivirus TAP1 to prostate cancer cells in a xenograft mouse model can prolong survival and increase the CD4⁺ T cells, and CD8⁺ T cells as well as decrease Foxp3⁺ T cells in the tumor microenvironment. In summary, a recombinant lentivirus expressing TAP1 can effectively increase prostate cancer tumor-specific immune response.
authors.affiliation: Department of Urology, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Qiu
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Xiu-heng
authors.initials: XH
authors.lastname: Liu
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Weng
authors.forename: You-lin
authors.initials: YL
authors.lastname: Kuang
authors.forename: Zhi-yuan
authors.initials: ZY
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Heng-cheng
authors.initials: HC
authors.lastname: Zhu
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000032:analysis; D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D000818:Animals; D017951:Antigen Presentation; D017209:Apoptosis; D017252:CD3 Complex / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D018920:Coculture Techniques; D051858:Forkhead Transcription Factors / Q000096:biosynthesis; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D006801:Humans; D016086:Lentivirus; D008297:Male; D026901:Membrane Transport Proteins / Q000378:metabolism*; D051379:Mice; D011471:Prostatic Neoplasms / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014183:Transplantation, Heterologous; D059016:Tumor Microenvironment
chemical_list: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters; D017252:CD3 Complex; D016827:CD8 Antigens; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D015395:Histocompatibility Antigens Class I; D026901:Membrane Transport Proteins; C073119:TAP1 protein, human; C107762:tapasin; D051997:ADP-ribosyl Cyclase 1