title: [Determination of artemisinin in rat plasma with HPLC-mS and its application].
abstract: OBJECTIVE
  To develop a method for determining artemisinin in rat plasma in vivo.
  
  
  METHODS
  HPLC-MS was adopted. Estazolam was selected as an internal standard (I.S.). The sample and I.S. were extracted using methyl tertbutyl ether and measured at m/z of 305 and 296, respectively.
  
  
  RESULTS
  Within the linear range of 5-500 microg x L(-1), the ratio of artemisinin's peak area and I.S. peak area and the concentration showed good linearity, thus the minimum concentration was set to be 5 mictrog x L(-1).
  
  
  CONCLUSIONS
  The methodology proved that the method is so suitable for determining the drug concentration in rat blood that it can be used for studying pharmacokinetics in animals.
authors.affiliation: West China School of Pharmacy, Sichuan University, Chengdu 610041, China. 281558607@qq.com
authors.forename: Chuan
authors.initials: C
authors.lastname: Fu
authors.forename: Jiying
authors.initials: J
authors.lastname: Yu
authors.forename: Jing
authors.initials: J
authors.lastname: Zou
authors.forename: Lin
authors.initials: L
authors.lastname: He
authors.forename: Yun
authors.initials: Y
authors.lastname: Dong
authors.forename: Yuan
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000818:Animals; D037621:Artemisinins / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D051381:Rats
chemical_list: D037621:Artemisinins; C031327:artemisinin