title: Eradication of hepatitis C virus subgenomic replicon by interferon results in aberrant retinol-related protein expression.
abstract: Hepatitis C virus (HCV) infection induces several changes in hepatocytes, such as oxidative stress, steatosis, and hepatocarcinogenesis. Although considerable progress has been made during recent years, the mechanisms underlying these functions remain unclear. We employed proteomic techniques in HCV replicon-harboring cells to determine the effects of HCV replication on host-cell protein expression. We examined two-dimensional electrophoresis (2-DE) and mass spectrometry to compare and identify differentially expressed proteins between HCV subgenomic replicon-harboring cells and their "cured" cells. One of the identified proteins was confirmed using enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. Full-length HCV genome RNA replicating and cured cells were also assessed using ELISA. Replicon-harboring cells showed higher expression of retinal dehydrogenase 1 (RALDH-1), which converts retinol to retinoic acid, and the cured cells showed higher expression of retinol-binding protein (RBP), which transports retinol from the liver to target tissues. The alteration in RBP expression was also confirmed by ELISA and Western blot analysis. We conclude that protein expression profiling demonstrated that HCV replicon eradication affected retinol-related protein expression.
authors.affiliation: Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan.
authors.forename: Kazuko
authors.initials: K
authors.lastname: Koike
authors.forename: Akinobu
authors.initials: A
authors.lastname: Takaki
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kato
authors.forename: Mamoru
authors.initials: M
authors.lastname: Ouchida
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Kanzaki
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yasunaka
authors.forename: Hidenori
authors.initials: H
authors.lastname: Shiraha
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Miyake
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D020869:Gene Expression Profiling; D016174:Hepacivirus / Q000235:genetics* / Q000378:metabolism; D006526:Hepatitis C / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D022781:Hepatocytes / Q000378:metabolism* / Q000821:virology; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D013058:Mass Spectrometry; D040901:Proteomics / Q000379:methods*; D012093:Replicon; D050697:Retinal Dehydrogenase / Q000378:metabolism*; D012177:Retinol-Binding Proteins / Q000378:metabolism*
chemical_list: D016898:Interferon-alpha; D012177:Retinol-Binding Proteins; D050697:Retinal Dehydrogenase