title: Enhanced susceptibility to Citrobacter rodentium infection in microRNA-155-deficient mice.
abstract: MicroRNAs (miRNAs) are small noncoding molecules that control gene expression posttranscriptionally, with microRNA-155 (miR-155) one of the first to be implicated in immune regulation. Here, we show that miR-155-deficient mice are less able to eradicate a mucosal Citrobacter rodentium infection than wild-type C57BL/6 mice. miR-155-deficient mice exhibited prolonged colonization associated with a higher C. rodentium burden in gastrointestinal tissue and spread into systemic tissues. Germinal center formation and humoral immune responses against C. rodentium were severely impaired in infected miR-155-deficient mice. A similarly susceptible phenotype was observed in μMT mice reconstituted with miR-155-deficient B cells, indicating that miR-155 is required intrinsically for mediating protection against this predominantly luminal bacterial pathogen.
authors.affiliation: The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom. sc7@sanger.ac.uk
authors.forename: Simon
authors.initials: S
authors.lastname: Clare
authors.forename: Victoria
authors.initials: V
authors.lastname: John
authors.forename: Alan W
authors.initials: AW
authors.lastname: Walker
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Hill
authors.forename: Cei
authors.initials: C
authors.lastname: Abreu-Goodger
authors.forename: Christine
authors.initials: C
authors.lastname: Hale
authors.forename: David
authors.initials: D
authors.lastname: Goulding
authors.forename: Trevor D
authors.initials: TD
authors.lastname: Lawley
authors.forename: Pietro
authors.initials: P
authors.lastname: Mastroeni
authors.forename: Gadi
authors.initials: G
authors.lastname: Frankel
authors.forename: Anton J
authors.initials: AJ
authors.lastname: Enright
authors.forename: Elena
authors.initials: E
authors.lastname: Vigorito
authors.forename: Gordon
authors.initials: G
authors.lastname: Dougan
mesh_terms: D000818:Animals; D044082:Citrobacter rodentium; D003092:Colitis / Q000235:genetics / Q000382:microbiology / Q000473:pathology; D004756:Enterobacteriaceae Infections / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D020022:Genetic Predisposition to Disease; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome
chemical_list: D035683:MicroRNAs; C532623:Mirn155 microRNA, mouse; D012336:RNA, Ribosomal, 16S