title: Chromatic analysis of teeth exposed to different mouthrinses.
abstract: OBJECTIVE
  The aim of this study was to assess, in vitro, the color of teeth exposed to different mouthrinses for a prolonged period.
  
  
  METHODS
  Bovine teeth were distributed in four groups: control, alcohol-containing mouthrinse (Listerine(®)), alcohol-free mouthrinse (Oral-B(®)) and chlorhexidine mouthrinse (Periogard(®)). The teeth were submitted to two cycles of staining and artificial aging. Color evaluation was performed with a digital spectrophotometer at the beginning of the experiment and after every cycle. Color changes were characterised using the system defined by the Comission Internationale de L'Eclairage (CIE L*, a*, b*). Data were analysed using the ANOVA and Tukey's post hoc test.
  
  
  RESULTS
  After the two cycles of staining and artificial aging, ΔE, ΔL and Δb from the alcohol-containing mouthrinse showed statistically significant differences when compared to the other groups. The ΔE values of the Listerine(®) group after the two cycles were greater than 3.7, indicating a visually perceptible color change.
  
  
  CONCLUSIONS
  The teeth exposed to the alcohol-containing mouthwash Listerine(®) were the only ones that presented a clinically perceptible color change.
  
  
  CONCLUSIONS
  A blue-colored alcohol-containing mouthwash was shown to be capable of causing dental color change after a prolonged period of exposure. Special care must be taken when choosing and prescribing the prolonged use of the same mouthwash.
authors.affiliation: Department of Pediatric Dentistry and Orthodontics, Universidade Federal do Rio de Janeiro, Brazil.
authors.forename: Alana Dantas
authors.initials: AD
authors.lastname: Moreira
authors.forename: Claudia Trindade
authors.initials: CT
authors.lastname: Mattos
authors.forename: Marcus Vinicius Almeida
authors.initials: MV
authors.lastname: de Araújo
authors.forename: Antônio Carlos de Oliveira
authors.initials: AC
authors.lastname: Ruellas
authors.forename: Eduardo Franzotti
authors.initials: EF
authors.lastname: Sant'anna
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D002417:Cattle; D002594:Cetylpyridinium / Q000494:pharmacology; D002710:Chlorhexidine / Q000494:pharmacology; D003116:Color; D003743:Dental Enamel / Q000187:drug effects*; D004338:Drug Combinations; D000431:Ethanol / Q000494:pharmacology; D006358:Hot Temperature; D006813:Humidity; D009067:Mouthwashes / Q000494:pharmacology*; D012459:Salicylates / Q000494:pharmacology; D012997:Solvents / Q000494:pharmacology; D013053:Spectrophotometry / Q000379:methods; D013729:Terpenes / Q000494:pharmacology; D013997:Time Factors; D019228:Tooth Crown / Q000187:drug effects; D014075:Tooth Discoloration / Q000139:chemically induced*; D014466:Ultraviolet Rays
chemical_list: D000891:Anti-Infective Agents, Local; D004338:Drug Combinations; D009067:Mouthwashes; D012459:Salicylates; D012997:Solvents; D013729:Terpenes; D000431:Ethanol; C057473:Listerine; D002594:Cetylpyridinium; D002710:Chlorhexidine