title: Grape-seed proanthocyanidin extract as suppressors of bone destruction in inflammatory autoimmune arthritis.
abstract: Chronic autoimmune inflammation, which is commonly observed in rheumatoid arthritis (RA), disrupts the delicate balance between bone resorption and formation causing thedestruction of the bone and joints. We undertook this study to verify the effects of natural grape-seed proanthocyanidin extract (GSPE), an antioxidant, on chronic inflammation and bone destruction. GSPE administration ameliorated the arthritic symptoms of collagen-induced arthritis (CIA), which are representative of cartilage and bone destruction. GSPE treatment reduced the formation of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells and osteoclast activity and increased differentiation of mature osteoblasts. Receptor activator of NFκB ligand expression in fibroblasts from RA patients was abrogated with GSPE treatment. GSPE blocked human peripheral blood mononuclear cell-derived osteoclastogenesis and acted as an antioxidant. GSPE improved the arthritic manifestations of CIA mice by simultaneously suppressing osteoclast differentiation and promoting osteoblast differentiation. Our results suggest that GSPE may be beneficial for the treatment of inflammation-associated bone destruction.
authors.affiliation: The Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Jin-Sil
authors.initials: JS
authors.lastname: Park
authors.forename: Mi-Kyung
authors.initials: MK
authors.lastname: Park
authors.forename: Hye-Joa
authors.initials: HJ
authors.lastname: Oh
authors.forename: Yun-Ju
authors.initials: YJ
authors.lastname: Woo
authors.forename: Mi-Ae
authors.initials: MA
authors.lastname: Lim
authors.forename: Jong-Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Ji Hyeon
authors.initials: JH
authors.lastname: Ju
authors.forename: Young Ok
authors.initials: YO
authors.lastname: Jung
authors.forename: Zang Hee
authors.initials: ZH
authors.lastname: Lee
authors.forename: Sung-Hwan
authors.initials: SH
authors.lastname: Park
authors.forename: Ho-Youn
authors.initials: HY
authors.lastname: Kim
authors.forename: Mi-La
authors.initials: ML
authors.lastname: Cho
authors.forename: Jun-Ki
authors.initials: JK
authors.lastname: Min
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001169:Arthritis, Experimental / Q000517:prevention & control*; D001172:Arthritis, Rheumatoid / Q000517:prevention & control*; D001483:Base Sequence; D001862:Bone Resorption; D001842:Bone and Bones / Q000187:drug effects* / Q000473:pathology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D017931:DNA Primers; D056604:Grape Seed Extract / Q000494:pharmacology*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D010006:Osteoblasts / Q000187:drug effects / Q000201:enzymology; D010010:Osteoclasts / Q000187:drug effects / Q000201:enzymology; D044945:Proanthocyanidins / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D017931:DNA Primers; D056604:Grape Seed Extract; C511402:Grape Seed Proanthocyanidins; D007527:Isoenzymes; D044945:Proanthocyanidins; D000135:Acid Phosphatase; C000605511:Acp5 protein, mouse; D000071681:Tartrate-Resistant Acid Phosphatase