title: Evaluation of genotoxicity in leukocytes and testis following intra-vasal contraception with RISUG and its reversal by DMSO and NaHCO₃ in Wistar albino rats.
abstract: Evaluation of genotoxicity of RISUG® - a vas based contraceptive, was carried out in the present study. Animals were allotted into groups of sham operated control, vas occlusion with RISUG (5-7 μl) for 360 days and reversal by DMSO (250-500 μl) and 5% NaHCO₃ (500 μl). Blood samples and testis were collected at 360 days of vas occlusion and 90 days of vas occlusion reversal for comet analysis. Hydrogen peroxide induced samples were used as positive control. Olive moment, tail length and percentage DNA in tail were recorded with minimum variation in all groups for both leukocytes and testis. When compared with positive control the variation was highly significant for both 20 μM and 50 μM H₂O₂ (p<0.001). It is concluded that vas occlusion with RISUG at the contraceptive dose regimen is not associated with genotoxicity in leukocytes or the testis of pre- and post-reversal rats.
authors.affiliation: Centre for Advanced Studies, Department of Zoology, University of Rajasthan, Jaipur 302004, India.
authors.forename: A S
authors.initials: AS
authors.lastname: Ansari
authors.forename: I
authors.initials: I
authors.lastname: Alam
authors.forename: M
authors.initials: M
authors.lastname: Hussain
authors.forename: S R
authors.initials: SR
authors.lastname: Khan
authors.forename: N K
authors.initials: NK
authors.lastname: Lohiya
mesh_terms: D000818:Animals; D002021:Buffers; D020552:Comet Assay; D003272:Contraceptive Agents, Male / Q000008:administration & dosage / Q000009:adverse effects* / Q000037:antagonists & inhibitors / Q000494:pharmacology; D004249:DNA Damage; D004121:Dimethyl Sulfoxide / Q000009:adverse effects* / Q000494:pharmacology; D005298:Fertility / Q000187:drug effects; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008845:Microinjections; D009152:Mutagenicity Tests; D011091:Polyesters / Q000008:administration & dosage / Q000009:adverse effects* / Q000494:pharmacology; D011137:Polystyrenes / Q000008:administration & dosage / Q000009:adverse effects* / Q000037:antagonists & inhibitors / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D055101:Semen Analysis; D017693:Sodium Bicarbonate / Q000009:adverse effects* / Q000494:pharmacology; D012997:Solvents / Q000009:adverse effects / Q000494:pharmacology; D013243:Sterilization Reversal; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D014649:Vas Deferens / Q000187:drug effects / Q000601:surgery
chemical_list: D002021:Buffers; D003272:Contraceptive Agents, Male; D011091:Polyesters; D011137:Polystyrenes; D012997:Solvents; C440495:contraceptive agent, RISUG; D017693:Sodium Bicarbonate; D004121:Dimethyl Sulfoxide