title: Role of nociceptive arcuate nucleus neurons in chloroquine-induced pruritic behaviors in mice.
abstract: Despite its clinical importance, the underlying central mechanisms of pruritic behaviors are poorly understood. To investigate the role of nociceptive arcuate nucleus neurons in chloroquine-induced pruritic behaviors in mice, we tested the effect of arcuate nucleus neurons and interscapular brown adipose tissue (IBAT) on itch produced by intradermal injection of chloroquine in the nape of the neck. Our results provide several lines of evidence for an important role of nociceptive arcuate nucleus neurons in chloroquine-induced pruritic behavior: (1) Intradermal microinjection of chloroquine resulted in a dramatic increase in itch behaviors accompanied by the activation of c-Fos positive neurons in arcuate nucleus; (2) Microinjection of chloroquine significantly increased IBAT temperature in the mice. These findings suggested that chloroquine-induced pruritic behaviors were associated with the activity of nociceptive arcuate nucleus neurons.
authors.affiliation: Medical Association of Hubei Province, Wuhan, 430060, China.
authors.forename: Yongtang
authors.initials: Y
authors.lastname: Song
authors.affiliation: Medical Association of Hubei Province, Wuhan, 430060, China.
authors.forename: Xuchu
authors.initials: X
authors.lastname: Pan
authors.affiliation: Department of Anesthesiology and Pain Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology and Pain Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. xianghongbing@gmail.com.
authors.forename: Hongbing
authors.initials: H
authors.lastname: Xiang
mesh_terms: D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000187:drug effects / Q000503:physiopathology*; D002738:Chloroquine / Q000009:adverse effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009619:Nociceptors / Q000187:drug effects / Q000502:physiology*; D011537:Pruritus / Q000139:chemically induced* / Q000503:physiopathology*
chemical_list: D002738:Chloroquine