title: The multigene family of lysophosphatidate acyltransferase (LPAT)-related enzymes in Ricinus communis: cloning and molecular characterization of two LPAT genes that are expressed in castor seeds.
abstract: The multigene family encoding proteins related to lysophosphatidyl-acyltransferases (LPATs) has been analyzed in the castor plant Ricinus communis. Among them, two genes designated RcLPAT2 and RcLPATB, encoding proteins with LPAT activity and expressed in the developing seed, have been cloned and characterized in some detail. RcLPAT2 groups with well characterized members of the so-called A-class LPATs and it shows a generalized expression pattern in the plant and along seed development. Enzymatic assays of RcLPAT2 indicate a preference for ricinoleoyl-CoA over other fatty acid thioesters when ricinoleoyl-LPA is used as the acyl acceptor, while oleoyl-CoA is the preferred substrate when oleoyl-LPA is employed. RcLPATB groups with B-class LPAT enzymes described as seed specific and selective for unusual fatty acids. However, RcLPATB exhibit a broad specificity on the acyl-CoAs, with saturated fatty acids (12:0-16:0) being the preferred substrates. RcLPATB is upregulated coinciding with seed triacylglycerol accumulation, but its expression is not restricted to the seed. These results are discussed in the light of a possible role for LPAT isoenzymes in the channelling of ricinoleic acid into castor bean triacylglycerol.
authors.affiliation: Grupo de Biotecnología de Productos Naturales (BIO-279), Centro de Investigación en Biotecnología Agroalimentaria, Campus de Excelencia Internacional Agroalimentario (CeiA3), Universidad de Almería, Almería, Spain.
authors.forename: José María
authors.initials: JM
authors.lastname: Arroyo-Caro
authors.forename: Tarik
authors.initials: T
authors.lastname: Chileh
authors.forename: Michael
authors.initials: M
authors.lastname: Kazachkov
authors.forename: Jitao
authors.initials: J
authors.lastname: Zou
authors.forename: Diego López
authors.initials: DL
authors.lastname: Alonso
authors.forename: Federico
authors.initials: F
authors.lastname: García-Maroto
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism; D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002367:Ricinus communis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D002368:Castor Oil / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D035264:Flowers / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D018547:Plant Stems / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D012277:Ricinoleic Acids / Q000378:metabolism; D012278:Ricinus / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity; D014280:Triglycerides / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D000214:Acyl Coenzyme A; D005227:Fatty Acids; D010940:Plant Proteins; D012277:Ricinoleic Acids; D014280:Triglycerides; D002368:Castor Oil; D000217:Acyltransferases; C018764:2-acylglycerophosphate acyltransferase; C030521:ricinoleic acid