title: Phosphoprotein staining for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using fluorescent reagent morin hydrate.
abstract: A fluorescence-based stain with 3,5,7,2',4'-pentahydroxyflavone (morin hydrate, MH) was designed to stain phosphoproteins in one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Al(3+) was applied as a "fixed bridge," providing an efficient energy transfer channel between phosphoprotein and MH, to produce a strong fluorescent complex for the determination of phosphoprotein. As little as 62.5ng of α-casein (7 or 8 phosphates) and β-casein (5 phosphates), 125ng of ovalbumin (2 phosphates), and κ-casein (1 phosphate) could be visualized with a wide linear dynamic range. In comparison with conventional methods, MH stain is a time-saving method that takes just 90min. It also has good compatibility with routine protein stainings such as Coomassie Brilliant Blue R (CBBR) and SYPRO Ruby for total protein analysis.
authors.affiliation: College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Gwangju 500-757, South Korea.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Hwang
authors.forename: Wei-Tao
authors.initials: WT
authors.lastname: Cong
authors.forename: Li-Tai
authors.initials: LT
authors.lastname: Jin
authors.forename: Jung-Kap
authors.initials: JK
authors.lastname: Choi
mesh_terms: D000818:Animals; D002364:Caseins / Q000032:analysis; D002417:Cattle; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005419:Flavonoids / Q000737:chemistry*; D005453:Fluorescence; D007202:Indicators and Reagents; D010047:Ovalbumin / Q000032:analysis; D010750:Phosphoproteins / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods; D047309:Flavones
chemical_list: D002364:Caseins; D005419:Flavonoids; D007202:Indicators and Reagents; D010047:Ovalbumin; D010750:Phosphoproteins; C008548:morin; D047309:Flavones