title: Association analyses of DNA polymorphisms in bovine SREBP-1, LXRα, FADS1 genes with fatty acid composition in Canadian commercial crossbred beef steers.
abstract: Two previously reported DNA polymorphisms of sterol regulatory element binding transcription factor 1 (SREBP-1) and liver X receptor alpha (LXRα) and two DNA polymorphisms of fatty acid desaturase 1 (FADS1) were evaluated for associations with fatty acids in brisket adipose tissue of Canadian crossbred beef steers. The polymorphism of 84 bp insert/deletion in intron 5 of SREBP-1 was significantly associated with the concentration of 9c C17:1 (P=0.013). The G>A single nucleotide polymorphism (SNP) in the exon 4 of LXRα gene was associated with the concentration of 9c, 11t C18:2 (P=0.04), sum of conjugated linoleic acids (CLA) (P=0.025) and 11c C20:1(P=0.042). Two DNA polymorphisms in the promoter region of FADS1, deletion/insertion of →GTG in rs133053720 and SNP A>G in rs42187276, were significantly associated with concentrations of C17:0 iso, C17:0 ai, total branched chain fatty acids (BFA), 12t C18:1, 13t/14t C18:1, 15t C18:1, and 13c C18:1 (P<0.05). Further studies are needed to validate the associations and to delineate the roles of the gene polymorphisms in determining the fatty acid composition in beef tissues.
authors.affiliation: College of Animal science, Tarim University, Alar, Xinjiang, 843300, China.
authors.forename: C
authors.initials: C
authors.lastname: Han
authors.forename: M
authors.initials: M
authors.lastname: Vinsky
authors.forename: N
authors.initials: N
authors.lastname: Aldai
authors.forename: M E R
authors.initials: ME
authors.lastname: Dugan
authors.forename: T A
authors.initials: TA
authors.lastname: McAllister
authors.forename: C
authors.initials: C
authors.lastname: Li
mesh_terms: D000818:Animals; D001947:Breeding; D002170:Canada; D002417:Cattle; D003132:Commerce; D004247:DNA; D005091:Exons; D044943:Fatty Acid Desaturases / Q000235:genetics*; D005227:Fatty Acids / Q000378:metabolism*; D007438:Introns; D000071518:Liver X Receptors; D008297:Male; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal; D009154:Mutation; D057093:Orphan Nuclear Receptors / Q000235:genetics*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics*
chemical_list: D005227:Fatty Acids; D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors; D051780:Sterol Regulatory Element Binding Protein 1; D004247:DNA; D044943:Fatty Acid Desaturases