title: MiR-17-3p inhibits angiogenesis by downregulating flk-1 in the cell growth signal pathway.
abstract: MicroRNAs (miRs) are endogenously expressed small noncoding RNAs that regulate gene expression at the posttranscriptional level. Previous works indicated that the miR-17-92 cluster could regulate endothelial cell (EC) functions involved in angiogenesis. miR-17-3p, a component of the miR-17-92 cluster, could control the angiogenic activity of human umbilical vein ECs in a cell-autonomous manner in vitro. A 21-bp fragment from the Flk-1 3'-untranslated region containing miR-17-3p targeting sites was required for the rapid downregulation of Flk-1 expression by in silico and experimental analysis. Subsequently, the downstream cell growth pathway was inhibited by forced upregulation of miR-17-3p. Based on these data, we conclude that miR-17-3p is a negative regulator of the angiogenic phenotype of ECs through its ability to modulate the expression of Flk-1, which is implicated in the pleiotropic effects of miR-17-92 in angiogenesis.
authors.affiliation: Department of Pharmacology, Nantong University Medical College, Nantong, PR China.
authors.forename: Runting
authors.initials: R
authors.lastname: Yin
authors.forename: Rubing
authors.initials: R
authors.lastname: Wang
authors.forename: Le
authors.initials: L
authors.lastname: Guo
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Lu
mesh_terms: D001483:Base Sequence; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D015536:Down-Regulation; D042783:Endothelial Cells / Q000502:physiology*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D057809:HEK293 Cells; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000503:physiopathology; D018919:Neovascularization, Physiologic / Q000235:genetics / Q000502:physiology*; D061251:Primary Cell Culture; D012313:RNA / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology*; D014162:Transfection; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*
chemical_list: C524941:MIRN17 microRNA, human; D035683:MicroRNAs; C039919:RNA, recombinant; D012313:RNA; D040301:Vascular Endothelial Growth Factor Receptor-2