title: Implementation of filmarray respiratory viral panel in a core laboratory improves testing turnaround time and patient care.
abstract: The FilmArray respiratory virus panel detects 15 viral agents in respiratory specimens using polymerase chain reaction. We performed FilmArray respiratory viral testing in a core laboratory at a regional children's hospital that provides service 24 hours a day 7 days a week. The average and median turnaround time were 1.6 and 1.4 hours, respectively, in contrast to 7 and 6.5 hours documented 1 year previously at an on-site reference laboratory using a direct fluorescence assay (DFA) that detected 8 viral agents. During the study period, rhinovirus was detected in 20% and coronavirus in 6% of samples using FilmArray; these viruses would not have been detected with DFA. We followed 97 patients with influenza A or influenza B who received care at the emergency department (ED). Overall, 79 patients (81%) were given oseltamivir in a timely manner defined as receiving the drug in the ED, a prescription in the ED, or a prescription within 3 hours of ED discharge. Our results demonstrate that molecular technology can be successfully deployed in a nonspecialty, high-volume, multidisciplinary core laboratory.
authors.affiliation: Dept of Laboratories, Seattle Children's Hospital, Seattle, WA 98105, USA. min.xu@seattlechildrens.org
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.forename: Xuan
authors.initials: X
authors.lastname: Qin
authors.forename: Michael L
authors.initials: ML
authors.lastname: Astion
authors.forename: Joe C
authors.initials: JC
authors.lastname: Rutledge
authors.forename: Joanne
authors.initials: J
authors.lastname: Simpson
authors.forename: Keith R
authors.initials: KR
authors.lastname: Jerome
authors.forename: Janet A
authors.initials: JA
authors.lastname: Englund
authors.forename: Danielle M
authors.initials: DM
authors.lastname: Zerr
authors.forename: Russell T
authors.initials: RT
authors.lastname: Migita
authors.forename: Shannon
authors.initials: S
authors.lastname: Rich
authors.forename: John C
authors.initials: JC
authors.lastname: Childs
authors.forename: Anne
authors.initials: A
authors.lastname: Cent
authors.forename: Mark A
authors.initials: MA
authors.lastname: Del Beccaro
mesh_terms: D000293:Adolescent; D000956:Antigens, Viral / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D017934:Coronavirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D042241:Early Diagnosis; D006801:Humans; D007223:Infant; D009980:Influenza A virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D009981:Influenza B virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D025202:Molecular Diagnostic Techniques; D060885:Multiplex Polymerase Chain Reaction; D012328:RNA Viruses / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000821:virology; D012229:Rhinovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D013997:Time Factors; D014773:Virology / Q000379:methods*; D014777:Virus Diseases / Q000175:diagnosis* / Q000821:virology; D055815:Young Adult
chemical_list: D000956:Antigens, Viral; D012367:RNA, Viral