title: Efficacy of collagen membrane seeded with autologous gingival fibroblasts in gingival recession treatment: a randomized, controlled pilot study.
abstract: BACKGROUND
  Gingival recession (GR) is one of the most common esthetic concerns associated with periodontal tissues. Recently, tissue engineering technology has been developed and applied in periodontology for the treatment of GR. The aim of this study is to compare the clinical efficacy of collagen membrane with or without autologous gingival fibroblasts under a coronally advanced flap for root coverage.
  
  
  METHODS
  In this split-mouth, controlled clinical study, 22 sites are selected from 11 patients with Miller Class I recessions affecting canines or premolars in the maxillary arch. One tooth in each patient was randomized to receive either a collagen membrane (CM) (control group) or a collagen membrane seeded with autologous gingival fibroblasts (CM+GF) (test group) under a coronally advanced flap. Thickness of the gingiva, GR, and percentage of root coverage (PRC) were recorded by a calibrated examiner at baseline and 3, 6, and 12 months postoperatively. Furthermore, GR and PRC were evaluated using photogrammetric analysis at baseline and 3, 6, and 12 months.
  
  
  RESULTS
  Both treatments resulted in a significant gain in root coverage compared with baseline. A statistically significant increase was detected in PRC in the test group compared with the control group. No significant difference was noted between the test and control sites regarding the thickness of the gingiva.
  
  
  CONCLUSIONS
  The results indicated that CM+GF prepared by tissue engineering technology can be considered an alternative method for the treatment of Miller Class I recession defects.
authors.affiliation: Department of Periodontology, Faculty of Dentistry, İzmir Katip Çelebi University, İzmir, Turkey.
authors.forename: Serhat
authors.initials: S
authors.lastname: Köseoğlu
authors.forename: İsmet
authors.initials: İ
authors.lastname: Duran
authors.forename: Mehmet
authors.initials: M
authors.lastname: Sağlam
authors.forename: S Buket
authors.initials: SB
authors.lastname: Bozkurt
authors.forename: Osman S
authors.initials: OS
authors.lastname: Kırtıloğlu
authors.forename: Sema S
authors.initials: SS
authors.lastname: Hakkı
mesh_terms: D000328:Adult; D064592:Autografts / Q000637:transplantation*; D001706:Biopsy / Q000379:methods; D003094:Collagen; D003774:Dental Plaque Index; D003807:Dentin Sensitivity / Q000145:classification; D005260:Female; D005347:Fibroblasts / Q000637:transplantation*; D005500:Follow-Up Studies; D005881:Gingiva / Q000166:cytology*; D005889:Gingival Recession / Q000601:surgery*; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D017622:Periodontal Attachment Loss / Q000145:classification; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000145:classification; D010780:Photogrammetry / Q000379:methods; D010865:Pilot Projects; D013524:Surgical Flaps / Q000601:surgery; D023822:Tissue Engineering / Q000379:methods; D019237:Tooth Cervix / Q000473:pathology; D014092:Tooth Root / Q000601:surgery; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D008567:Membranes, Artificial; D003094:Collagen