title: Characterization of new DOPC/DHPC platform for dermal applications.
abstract: Systems formed by mixtures of the phospholipids dioleoylphosphatidylcholine (DOPC) and dihexanoylphosphatidylcholine (DHPC) were characterized by use of differential scanning calorimetry, small angle X-ray scattering and two electron-microscopy techniques, freeze fracture electron microscopy and cryogenic transmission electron microscopy. These techniques allowed for the determination of the size, morphology, structural topology, self-assembly and thermotropic behavior of the nanostructures present in the mixtures. The interaction between the two phospholipids provides curvatures, irregularities and the increase of thickness and flexibility in the membrane. These effects led to the formation of different aggregates with a differential distribution of both phospholipids. The effect of these systems on the skin in vivo was evaluated by measurement of the biophysical skin parameters. Our results show that the DOPC/DHPC application induces a decrease in the permeability and in the hydration of the tissue. These effects in vivo are related to different microstructural changes promoted by these systems in the skin in vitro, published in a recent work. The fundamental biophysical analyses of DOPC/DHPC systems contribute to our understanding of the mechanisms that govern their interaction with the skin.
authors.affiliation: Department of Chemical and Surfactant Technology, Institute of Advanced Chemistry of Catalonia, IQAC-CSIC, C/Jordi Girona 18-26, 08034, Barcelona, Spain. gelen.rodriguez@cid.csic.es
authors.forename: Gelen
authors.initials: G
authors.lastname: Rodríguez
authors.forename: Laia
authors.initials: L
authors.lastname: Rubio
authors.forename: Clara
authors.initials: C
authors.lastname: Barba
authors.forename: Carmen
authors.initials: C
authors.lastname: López-Iglesias
authors.forename: Alfons
authors.initials: A
authors.lastname: de la Maza
authors.forename: Olga
authors.initials: O
authors.lastname: López
authors.forename: Mercedes
authors.initials: M
authors.lastname: Cócera
mesh_terms: D000328:Adult; D055592:Biophysical Phenomena / Q000187:drug effects; D004548:Elasticity; D006801:Humans; D008297:Male; D010713:Phosphatidylcholines / Q000737:chemistry* / Q000494:pharmacology*; D010742:Phospholipid Ethers / Q000737:chemistry* / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D013816:Thermodynamics; D044366:Transition Temperature; D014867:Water / Q000378:metabolism
chemical_list: C052298:1,2-dihexadecyl-sn-glycero-3-phosphocholine; D010713:Phosphatidylcholines; D010742:Phospholipid Ethers; D014867:Water; C017251:1,2-oleoylphosphatidylcholine