title: Mycobacterial and mouse HSP70 have immuno-modulatory effects on dendritic cells.
abstract: Previously, it has been shown that heat shock protein 70 (HSP70) can prevent inflammatory damage in experimental autoimmune disease models. Various possible underlying working mechanisms have been proposed. One possibility is that HSP70 induces a tolerogenic phenotype in dendritic cells (DCs) as a result of the direct interaction of the antigen with the DC. Tolerogenic DCs can induce antigen-specific regulatory T cells and dampen pathogenic T cell responses. We show that treatment of murine DCs with either mycobacterial (Mt) or mouse HSP70 and pulsed with the disease-inducing antigen induced suppression of proteoglycan-induced arthritis (PGIA), although mouse HSP70-treated DCs could ameliorate PGIA to a greater extent. In addition, while murine DCs treated with Mt- or mouse HSP70 had no significantly altered phenotype as compared to untreated DCs, HSP70-treated DCs pulsed with pOVA (ovalbumin peptide 323-339) induced a significantly increased production of IL-10 in pOVA-specific T cells. IL-10-producing T cells were earlier shown to be involved in Mt HSP70-induced suppression of PGIA. In conclusion, this study indicates that Mt- and mouse HSP70-treated BMDC can suppress PGIA via an IL-10-producing T cell-dependent manner.
authors.affiliation: Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Spiering
authors.forename: R
authors.initials: R
authors.lastname: van der Zee
authors.forename: J
authors.initials: J
authors.lastname: Wagenaar
authors.forename: W
authors.initials: W
authors.lastname: van Eden
authors.forename: F
authors.initials: F
authors.lastname: Broere
mesh_terms: D000818:Animals; D001168:Arthritis / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control; D001426:Bacterial Proteins / Q000378:metabolism / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000166:cytology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D019286:Endopeptidase K / Q000378:metabolism; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D009161:Mycobacterium / Q000378:metabolism; D010047:Ovalbumin / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D010641:Phenotype; D011509:Proteoglycans / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D016207:Cytokines; D018840:HSP70 Heat-Shock Proteins; C111390:OVA 323-339; D010446:Peptide Fragments; D011509:Proteoglycans; D016753:Interleukin-10; D010047:Ovalbumin; D019286:Endopeptidase K