title: [Influence of Bushen Huoxue decoction on beta-catenin, MMP-7 of synoviocytes in rats with knee osteoarthritis].
abstract: OBJECTIVE
  To observe beta-catenin expression of Wnt signaling pathway in rats with knee osteoarthritis, and influence of Bushen Huoxue decoction on beta-catenin and MMP-7 expression of synoviocytes in rats with knee osteoarthritis (OA).
  
  
  METHODS
  Rats model with knee osteoarthritis were established by Hulth method. Primary synoviocytes and OA synoviocytes were cultured with collagenase digestion method. The cultured synoviocytes were divided into normal group, OA model group and Bushen Huoxue decoction group. Western blotting method was used to detect beta-catenin, MMP-7 protein expression of synoviocytes after acting by Bushen Huoxue decoction for 48 h; ELISA method was used to detect MMP-7 expression of synovial supernatant.
  
  
  RESULTS
  OA synoviocytes were cultured successfully. Western blotting showed that beta-catenin, MMP-7 expression in OA synoviocytes was significantly higher than normal group, Bushen Huoxue decoction could significantly reduce beta-catenin, MMP-7 expression; ELISA results showed that MMP-7 expression of OA synovial supernatant was significantly higher than normal synoviocytes supernatant, Bushen Huoxue decoction significantly regulated the level MMP-7 down.
  
  
  CONCLUSIONS
  (1) High expression of beta-catenin in OA synoviocytes indicates that Wnt classical signal pathway is activated in rat with knee osteoarthritis; (2) High expression of MMP-7 expression in OA synoviocytes confirms the MMP-7 is downstream genes of Wnt/beta-catenin signal pathway; (3) Activation of Wnt signal pathway and increase of MMP-7 may cause degradation of articular cartilage, and promote the formation of osteoarthritis; (4) Bushen Huoxue decoction can reduce expression of MMP-7, and promote cartilage repair, which may be one of mechanisms of osteoarthritis.
authors.affiliation: Department of Orthopaedics, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yuan
authors.forename: Wei-Bing
authors.initials: WB
authors.lastname: Kan
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Song
authors.forename: Jin
authors.initials: J
authors.lastname: Zhao
authors.forename: Wei-Guo
authors.initials: WG
authors.lastname: Yu
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D020783:Matrix Metalloproteinase 7 / Q000032:analysis*; D020370:Osteoarthritis, Knee / Q000188:drug therapy* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013582:Synovial Fluid / Q000737:chemistry* / Q000166:cytology; D051176:beta Catenin / Q000032:analysis*
chemical_list: D004365:Drugs, Chinese Herbal; D051176:beta Catenin; C527851:bushen huoxue; D020783:Matrix Metalloproteinase 7