title: Mimicking nature: synthetic nicotinamide cofactors for C═C bioreduction using enoate reductases.
abstract: A series of synthetic nicotinamide cofactors were synthesized to replace natural nicotinamide cofactors and promote enoate reductase (ER) catalyzed reactions without compromising the activity or stereoselectivity of the bioreduction process. Conversions and enantioselectivities of >99% were obtained for C═C bioreductions, and the process was successfully upscaled. Furthermore, high chemoselectivity was observed when employing these nicotinamide cofactor mimics (mNADs) with crude extracts in ER-catalyzed reactions.
authors.affiliation: Department of Biotechnology, Delft University of Technology, Julianalaan 136, 2628BL Delft, The Netherlands.
authors.forename: Caroline E
authors.initials: CE
authors.lastname: Paul
authors.forename: Serena
authors.initials: S
authors.lastname: Gargiulo
authors.forename: Diederik J
authors.initials: DJ
authors.lastname: Opperman
authors.forename: Iván
authors.initials: I
authors.lastname: Lavandera
authors.forename: Vicente
authors.initials: V
authors.lastname: Gotor-Fernández
authors.forename: Vicente
authors.initials: V
authors.lastname: Gotor
authors.forename: Andreas
authors.initials: A
authors.lastname: Taglieber
authors.forename: Isabel W C E
authors.initials: IW
authors.lastname: Arends
authors.forename: Frank
authors.initials: F
authors.lastname: Hollmann
mesh_terms: D002384:Catalysis; D020650:Combinatorial Chemistry Techniques; D018716:Molecular Mimicry; D015394:Molecular Structure; D009243:NAD / Q000737:chemistry / Q000378:metabolism; D009536:Niacinamide / Q000138:chemical synthesis* / Q000737:chemistry; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000737:chemistry / Q000378:metabolism*
chemical_list: D009243:NAD; D009536:Niacinamide; D044925:Oxidoreductases Acting on CH-CH Group Donors; C019367:2-enoate reductase