title: Selective concentration of EPA and DHA using Thermomyces lanuginosus lipase is due to fatty acid selectivity and not regioselectivity.
abstract: The selectivity of anchovy oil hydrolysis was optimised for Thermomyces lanuginosus lipase, so that docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) were concentrated and partially separated from each other. Enzyme concentration and pH control were important factors for effective hydrolysis. Monitoring percent hydrolysis using capillary chromatography with flame ionisation detector (Iatroscan) and fatty acid selectivity using gas chromatography (GC) indicated that during hydrolysis DHA primarily remained on the glycerol backbone, while EPA was progressively removed. (13)C nuclear magnetic resonance (NMR) data showed that selectivity of hydrolysis was primarily due to fatty acid selectivity and not regioselectivity, with hydrolysis from both sn-1,3 and sn-2 sites being equally favoured.
authors.affiliation: School of Life and Environmental Sciences, Deakin University, Locked Bag 20000, Geelong, Victoria 3220, Australia.
authors.forename: Taiwo O
authors.initials: TO
authors.lastname: Akanbi
authors.forename: Jacqui L
authors.initials: JL
authors.lastname: Adcock
authors.forename: Colin J
authors.initials: CJ
authors.lastname: Barrow
mesh_terms: D001203:Ascomycota / Q000737:chemistry / Q000201:enzymology*; D004281:Docosahexaenoic Acids / Q000737:chemistry*; D015118:Eicosapentaenoic Acid / Q000737:chemistry*; D005227:Fatty Acids / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D006868:Hydrolysis; D008049:Lipase / Q000737:chemistry*; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D005227:Fatty Acids; D005656:Fungal Proteins; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid; D008049:Lipase