title: Calcyclin binding protein and Siah-1 interacting protein in Alzheimer's disease pathology: neuronal localization and possible function.
abstract: The calcyclin binding protein and Siah-1 interacting protein (CacyBP/SIP) protein was shown to play a role in the organization of microtubules. In this work we have examined the neuronal distribution and possible function of CacyBP/SIP in cytoskeletal pathophysiology. We have used brain tissue from Alzheimer's disease (AD) patients and from transgenic mice modeling 2 different pathologies characteristic for AD: amyloid and tau. In the brain from AD patients, CacyBP/SIP was found to be almost exclusively present in neuronal somata, and in control patients it was seen in the somata and neuronal processes. In mice doubly transgenic for amyloid precursor protein and presenilin 1 there was no difference in CacyBP/SIP neuronal localization in comparison with the nontransgenic animals. By contrast in tau transgenic mice, localization of CacyBP/SIP was similar to that observed for AD patients. To find the relation between CacyBP/SIP and tau we examined dephosphorylation of tau by CacyBP/SIP. We found that indeed it exhibited phosphatase activity toward tau. Altogether, our results suggest that CacyBP/SIP might play a role in AD pathology.
authors.affiliation: Nencki Institute of Experimental Biology, Warsaw, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Wasik
authors.forename: Gabriela
authors.initials: G
authors.lastname: Schneider
authors.forename: Anna
authors.initials: A
authors.lastname: Mietelska-Porowska
authors.forename: Marcin
authors.initials: M
authors.lastname: Mazurkiewicz
authors.forename: Hanna
authors.initials: H
authors.lastname: Fabczak
authors.forename: Serge
authors.initials: S
authors.lastname: Weis
authors.forename: Claudia
authors.initials: C
authors.lastname: Zabke
authors.forename: Charles R
authors.initials: CR
authors.lastname: Harrington
authors.forename: Anna
authors.initials: A
authors.lastname: Filipek
authors.forename: Grazyna
authors.initials: G
authors.lastname: Niewiadomska
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D006801:Humans; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism*; D014018:Tissue Distribution; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*; D000098563:Seven in Absentia Proteins
chemical_list: C491778:CACYBP protein, human; C111922:Cacybp protein, mouse; D002135:Calcium-Binding Proteins; D009687:Nuclear Proteins; D044767:Ubiquitin-Protein Ligases; D000098563:Seven in Absentia Proteins