title: From FRET imaging to practical methodology for kinase activity sensing in living cells.
abstract: Biological processes are intrinsically dynamic. Although traditional methods provide valuable insights for the understanding of many biological phenomena, the possibility of measuring, quantifying, and localizing proteins within a cell, a tissue, and even an embryo has revolutionized our train of thoughts and has encouraged scientists to develop molecular tools for the assessment of protein or protein complex dynamics within their physiological context. These ongoing efforts rest on the emergence of biophotonic techniques and the continuous improvement of fluorescent probes, allowing precise and reliable measurements of dynamic cellular functions. The march of the "in vivo biochemistry" has begun, already yielding breathtaking results.
authors.affiliation: Biophotonic Team (BCF), IRI CNRS, University Lille1-University Lille2 USR 3078, Villeneuve d'Ascq, France.
authors.forename: François
authors.initials: F
authors.lastname: Sipieter
authors.forename: Pauline
authors.initials: P
authors.lastname: Vandame
authors.forename: Corentin
authors.initials: C
authors.lastname: Spriet
authors.forename: Aymeric
authors.initials: A
authors.lastname: Leray
authors.forename: Pierre
authors.initials: P
authors.lastname: Vincent
authors.forename: Dave
authors.initials: D
authors.lastname: Trinel
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Bodart
authors.forename: Franck B
authors.initials: FB
authors.lastname: Riquet
authors.forename: Laurent
authors.initials: L
authors.lastname: Héliot
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D049109:Cell Proliferation; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006801:Humans; D010766:Phosphorylation; D010770:Phosphotransferases / Q000737:chemistry*; D017785:Photons; D011485:Protein Binding
chemical_list: D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D010770:Phosphotransferases