title: Serum peptidome profiling for identifying pathological patterns in patients with primary nephrotic syndrome.
abstract: BACKGROUND
  Renal biopsy is necessary for diagnosing the pathological changes of primary nephrotic syndrome (NS). However, it is invasive, time-consuming and can not be performed frequent on the same patient. Thus, development of a non-invasive and rapid diagnostic method may improve clinical patient management.
  
  
  METHODS
  Proteomic tool magnetic bead-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MB-based MALDI TOF MS) was applied to serum to determine peptidome patterns that are characteristic of different pathological changes.
  
  
  RESULTS
  Serum specimen from 114 patients with NS (62 were minimal change disease (MCD), 30 were membranous nephropathy (MN), and 22 were focal segmental glomerulosclerosis (FSGS)) and 60 normal individuals were analyzed using MB-based MALDI TOF MS. The peptidome pattern was generated by genetic algorithms using a training set of 31 MCD, 15 MN, 11 FSGS and 30 normal individuals and was validated by an independent testing set of the remaining samples. The serum peptidome pattern, based on a panel of 14 peaks, accurately recognized samples from MCD, MN, FSGS and healthy control with sensitivities of 93.5%, 86.7%, 63.6% and 90.0%, and specificities of 98.2%, 94.4%, 100% and 89.5%, respectively. Moreover, one peptide from peptidome pattern was identified by liquid chromatography tandem mass spectrometry (LC MS/MS) as fibrinogen A.
  
  
  CONCLUSIONS
  Detection of the serum peptidome pattern is a rapid, non-invasive, high-throughout, and reproducible method for identifying the pathological patterns of patients with nephrotic syndrome.
authors.affiliation: Department of Nephrology, Sun Yat-sen University, Guangzhou, Guangdong, China.
authors.forename: Lan-ting
authors.initials: LT
authors.lastname: Huang
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wen
authors.forename: Ming-zhe
authors.initials: MZ
authors.lastname: Zhao
authors.forename: Zhi-bin
authors.initials: ZB
authors.lastname: Li
authors.forename: Ning
authors.initials: N
authors.lastname: Luo
authors.forename: Yong-tao
authors.initials: YT
authors.lastname: Wang
authors.forename: Xiu-qing
authors.initials: XQ
authors.lastname: Dong
authors.forename: Xue-qing
authors.initials: XQ
authors.lastname: Yu
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009404:Nephrotic Syndrome / Q000097:blood*; D010455:Peptides / Q000097:blood*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D055815:Young Adult
chemical_list: D010455:Peptides