title: Lack of evidence to support the association of a single IL28B genotype SNP rs12979860 with the HTLV-1 clinical outcomes and proviral load.
abstract: BACKGROUND
  The Interleukin 28B (IL28B) rs12979860 polymorphisms was recently reported to be associated with the human T-cell leukemia virus type 1 (HTLV-1) proviral load (PvL) and the development of the HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP).
  
  
  METHODS
  In an attempt to examine this hypothesis, we assessed the association of the rs12979860 genotypes with HTLV-1 PvL levels and clinical status in 112 unrelated Brazilian subjects (81 HTLV-1 asymptomatic carriers, 24 individuals with HAM/TSP and 7 with Adult T cell Leukemia/Lymphoma (ATLL)).
  
  
  RESULTS
  All 112 samples were successfully genotyped and their PvLs compared. Neither the homozygote TT nor the heterozygote CT mutations nor the combination genotypes (TT/CT) were associated with a greater PvL. We also observed no significant difference in allele distribution between asymptomatic carriers and patients with HTLV-1 associated HAM/TSP.
  
  
  CONCLUSIONS
  Our study failed to support the previously reported positive association between the IL28B rs12979860 polymorphisms and an increased risk of developing HAM/TSP in the Brazilian population.
authors.affiliation: Clinical Laboratory, Department of Pathology, Hospital das Clínicas, School of Medicine, University of São Paulo, São Paulo, Brazil. sabyem_63@yahoo.com
authors.forename: Sabri Saeed
authors.initials: SS
authors.lastname: Sanabani
authors.forename: Youko
authors.initials: Y
authors.lastname: Nukui
authors.forename: Juliana
authors.initials: J
authors.lastname: Pereira
authors.forename: Antonio Charlys
authors.initials: AC
authors.lastname: da Costa
authors.forename: Ana Carolina Soares
authors.initials: AC
authors.lastname: de Oliveira
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Pessôa
authors.forename: Fabio Eudes
authors.initials: FE
authors.lastname: Leal
authors.forename: Aluisio C
authors.initials: AC
authors.lastname: Segurado
authors.forename: Esper Georges
authors.initials: EG
authors.lastname: Kallas
authors.forename: Ester Cerdeira
authors.initials: EC
authors.lastname: Sabino
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D005838:Genotype; D015490:HTLV-I Infections / Q000473:pathology* / Q000821:virology*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D007372:Interferons; D007378:Interleukins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D019562:Viral Load; D055815:Young Adult; D000095022:Interferon Lambda
chemical_list: D007372:Interferons; D007378:Interleukins; C000624353:interferon-lambda, human; D000095022:Interferon Lambda