title: Localization and expression profiling of a 31 kDa antigenic repetitive protein Sjp_0110390 in Schistosoma japonicum life stages.
abstract: Sj7TR is a 13 kDa repetitive region of a 31 kDa protein in Schistosoma japonicum known as Sjp_0110390 that showed high sensitivity and specificity in antibody detection against schistosomiasis patients. However, the current database for S. japonicum genes characterized it only as an expressed protein. A more thorough understanding of this antigenic protein is therefore necessary to possibly give more information about the nature of this protein and its role in the parasite. In this study, immunolocalization and expression profiling were done for Sjp_0110390 on the different stages of the parasite. Immunofluorescent assay showed that Sjp_0110390 was expressed in the young stages of the parasites including the schistosomula, eggs, aquatic and intra-molluscan stages. This was supported by the reverse-transcriptase PCR which confirmed the stage-specific expression of Sjp_0110390 and Western blot test which detected the protein in the extracted eggs proteins, but not in the adults. Furthermore, it was also highly expressed in infected Oncomelania hupensis nosophora snails suggesting that Sjp_0110390 might have a role in the development of the parasite inside the intermediate host. This result also suggests that Sj7TR might be used not only for human diagnosis but to detect snail infection as well.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
authors.forename: Jose Ma M
authors.initials: JM
authors.lastname: Angeles
authors.forename: Masashi
authors.initials: M
authors.lastname: Kirinoki
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Goto
authors.forename: Masahito
authors.initials: M
authors.lastname: Asada
authors.forename: Hassan
authors.initials: H
authors.lastname: Hakimi
authors.forename: Lydia R
authors.initials: LR
authors.lastname: Leonardo
authors.forename: Pilarita
authors.initials: P
authors.lastname: Tongol-Rivera
authors.forename: Elena A
authors.initials: EA
authors.lastname: Villacorte
authors.forename: Noboru
authors.initials: N
authors.lastname: Inoue
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Chigusa
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Kawazu
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D015153:Blotting, Western; D020869:Gene Expression Profiling; D006801:Humans; D008856:Microscopy, Fluorescence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012549:Schistosoma japonicum / Q000737:chemistry* / Q000235:genetics*; D012908:Snails / Q000469:parasitology; D015053:Zygote / Q000737:chemistry
chemical_list: D000947:Antigens, Helminth