title: Coupling osmium complexes to epoxy-functionalised polymers to provide mediated enzyme electrodes for glucose oxidation.
abstract: Newly synthesised osmium complex-modified redox polymers were tested for potential application as mediators in glucose oxidising enzyme electrodes for application to biosensors or biofuel cells. Coupling of osmium complexes containing amine functional groups to epoxy-functionalised polymers of variable composition provides a range of redox polymers with variation possible in redox potential and physicochemical properties. Properties of the redox polymers as mediators for glucose oxidation were investigated by co-immobilisation onto graphite with glucose oxidase or FAD-dependent glucose dehydrogenase using a range of crosslinkers and in the presence and absence of multiwalled carbon nanotubes. Electrodes prepared by immobilising [P20-Os(2,2'-bipyridine)2(4-aminomethylpyridine)Cl].PF6, carbon nanotubes and glucose oxidase exhibit glucose oxidation current densities as high as 560μAcm(-2) for PBS containing 100mM glucose at 0.45V vs. Ag/AgCl. Films prepared by crosslinking [P20-Os(4,4'-dimethoxy-2,2'-bipyridine)2(4-aminomethylpyridine)Cl].PF6, an FAD-dependent glucose dehydrogenase, and carbon nanotubes achieve current densities of 215μAcm(-2) in 5mM glucose at 0.2V vs. Ag/AgCl, showing some promise for application to glucose oxidising biosensors or biofuel cells.
authors.affiliation: School of Chemistry and Ryan Institute, National University of Ireland Galway, Galway, Ireland.
authors.forename: Peter
authors.initials: P
authors.lastname: Ó Conghaile
authors.forename: Sascha
authors.initials: S
authors.lastname: Pöller
authors.forename: Domhnall
authors.initials: D
authors.lastname: MacAodha
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schuhmann
authors.forename: Dónal
authors.initials: D
authors.lastname: Leech
mesh_terms: D001674:Bioelectric Energy Sources; D015374:Biosensing Techniques / Q000295:instrumentation; D003217:Conductometry / Q000295:instrumentation*; D004566:Electrodes; D004853:Epoxy Resins / Q000737:chemistry*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis* / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry
chemical_list: D004853:Epoxy Resins; D011108:Polymers; D005949:Glucose Oxidase; D005947:Glucose