title: Allosteric control in a metalloprotein dramatically alters function.
abstract: Metalloproteins (MPs) comprise one-third of all known protein structures. This diverse set of proteins contain a plethora of unique inorganic moieties capable of performing chemistry that would otherwise be impossible using only the amino acids found in nature. Most of the well-studied MPs are generally viewed as being very rigid in structure, and it is widely thought that the properties of the metal centers are primarily determined by the small fraction of amino acids that make up the local environment. Here we examine both theoretically and experimentally whether distal regions can influence the metal center in the diabetes drug target mitoNEET. We demonstrate that a loop (L2) 20 Å away from the metal center exerts allosteric control over the cluster binding domain and regulates multiple properties of the metal center. Mutagenesis of L2 results in significant shifts in the redox potential of the [2Fe-2S] cluster and orders of magnitude effects on the rate of [2Fe-2S] cluster transfer to an apo-acceptor protein. These surprising effects occur in the absence of any structural changes. An examination of the native basin dynamics of the protein using all-atom simulations shows that twisting in L2 controls scissoring in the cluster binding domain and results in perturbations to one of the cluster-coordinating histidines. These allosteric effects are in agreement with previous folding simulations that predicted L2 could communicate with residues surrounding the metal center. Our findings suggest that long-range dynamical changes in the protein backbone can have a significant effect on the functional properties of MPs.
authors.affiliation: Department of Chemistry and Biochemistry and Center for Theoretical Biological Physics, University of California at San Diego, La Jolla, CA 92093, USA.
authors.forename: Elizabeth Leigh
authors.initials: EL
authors.lastname: Baxter
authors.forename: John A
authors.initials: JA
authors.lastname: Zuris
authors.forename: Charles
authors.initials: C
authors.lastname: Wang
authors.forename: Phu Luong T
authors.initials: PL
authors.lastname: Vo
authors.forename: Herbert L
authors.initials: HL
authors.lastname: Axelrod
authors.forename: Aina E
authors.initials: AE
authors.lastname: Cohen
authors.forename: Mark L
authors.initials: ML
authors.lastname: Paddock
authors.forename: Rachel
authors.initials: R
authors.lastname: Nechushtai
authors.forename: Jose N
authors.initials: JN
authors.lastname: Onuchic
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Jennings
mesh_terms: D000494:Allosteric Regulation; D001665:Binding Sites; D055592:Biophysical Phenomena; D018360:Crystallography, X-Ray; D006639:Histidine / Q000737:chemistry; D007506:Iron-Sulfur Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008667:Metalloproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D010084:Oxidation-Reduction; D011487:Protein Conformation; D055550:Protein Stability
chemical_list: C525595:CISD1 protein, human; D007506:Iron-Sulfur Proteins; D008667:Metalloproteins; D024101:Mitochondrial Proteins; D006639:Histidine