title: Amounts and compositional analysis of glycosaminoglycans in the tissue of fish.
abstract: We isolated GAGs from the inedible parts; head, skin, internal organs, fins, scales and spine, of atlantic mackerel (Scomber scombrus), japanese jack mackerel (Trachurus japonicus), pacific bluefin tuna (Thunnus orientalis), yellowfin sole (Limanda aspera), broadbanded thornyhead (Sebastolobus macrochir), golden threadfin bream (Nemipterus virgatus), and nile tilapia (Oreochromis niloticus). We also investigated deep-sea fish, eelpouts (Bothrocara hollandi, Lycodes toyamensis, and Lycodes nakamurae), rough snailfish (Careproctus trachysoma), and squids (Watasenia scintillans, Enoploteuthis chunii, and Berryteuthis magister). Enzymatic digestion of the GAGs enabled a compositional analysis of CS, DS, and HA including the sulfation patterns of CS and DS, as well as the amount of each GAG. The molecular weights and distributions of these GAGs were also examined. The amounts of GAGs contained in the tissues and CS/DS ratios differed remarkably among the fish. The dorsal fin of the yellowfin sole contained more than 1300mg of CS-DS per 100g of defatted-dry tissue. Although the fish generally contained A-type rich CS-DS, bottom fish and deep-sea fish often possessed C-type CS-DS in larger ratios. Squid characteristically had E-type CS-DS which was normally less common in fish except in cartilaginous fish. These analytical results had no relation to the biological classification.
authors.affiliation: Department of Regional Environment, Faculty of Regional Sciences, Tottori University, Koyamacho-Minami 4-101, Tottori 680-8551, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Arima
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Fujita
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Toita
authors.forename: Ayaka
authors.initials: A
authors.lastname: Imazu-Okada
authors.forename: Nao
authors.initials: N
authors.lastname: Tsutsumishita-Nakai
authors.forename: Naoko
authors.initials: N
authors.lastname: Takeda
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Nakao
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Manami
authors.initials: M
authors.lastname: Kawano
authors.forename: Kenya
authors.initials: K
authors.lastname: Matsushita
authors.forename: Haruna
authors.initials: H
authors.lastname: Tanaka
authors.forename: Shin
authors.initials: S
authors.lastname: Morimoto
authors.forename: Ayumi
authors.initials: A
authors.lastname: Nakamura
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kitagaki
authors.forename: Yuka
authors.initials: Y
authors.lastname: Hieda
authors.forename: Ryuya
authors.initials: R
authors.lastname: Hatto
authors.forename: Ayako
authors.initials: A
authors.lastname: Watanabe
authors.forename: Takeru
authors.initials: T
authors.lastname: Yumura
authors.forename: Takashi
authors.initials: T
authors.lastname: Okuhara
authors.forename: Hiroki
authors.initials: H
authors.lastname: Hayashi
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Shimizu
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Nakayama
authors.forename: Shinya
authors.initials: S
authors.lastname: Masuda
authors.forename: Yukio
authors.initials: Y
authors.lastname: Ishihara
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Yoshioka
authors.forename: Shinobu
authors.initials: S
authors.lastname: Yoshioka
authors.forename: Seizo
authors.initials: S
authors.lastname: Shirade
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Tamura
mesh_terms: D000818:Animals; D005399:Fishes; D006025:Glycosaminoglycans / Q000032:analysis* / Q000737:chemistry*; D008970:Molecular Weight
chemical_list: D006025:Glycosaminoglycans