title: p13 from group II baculoviruses is a killing-associated gene.
abstract: p13 gene was first described in Leucania separata multinuclear polyhedrosis virus (Ls-p13) several years ago, but the function of P13 protein has not been experimentally investigated to date. In this article, we indicated that the expression of p13 from Heliothis armigera single nucleocapsid nucleopolyhedrovirus (Ha-p13) was regulated by both early and late promoter. Luciferase assay demonstrated that the activity of Ha-p13 promoter with hr4 enhancer was more than 100 times in heterologous Sf9 cells than that in nature host Hz-AM1 cells. Both Ls-P13 and Ha-P13 are transmembrane proteins. Confocal microscopic analysis showed that both mainly located in the cytoplasm membrane at 48 h. Results of RNA interference indicated that Ha-p13 was a killing-associated gene for host insects H. armigera. The AcMNPV acquired the mentioned killing activity and markedly accelerate the killing rate when expressing Ls-p13. In conclusion, p13 is a killing associated gene in both homologous and heterologous nucleopolyhedrovirus.
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Lu
authors.forename: Engi
authors.initials: E
authors.lastname: Du
authors.forename: Yangkun
authors.initials: Y
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Qiao
authors.forename: Lunguang
authors.initials: L
authors.lastname: Yao
authors.forename: Zishu
authors.initials: Z
authors.lastname: Pan
authors.forename: Songya
authors.initials: S
authors.lastname: Lu
authors.forename: Yipeng
authors.initials: Y
authors.lastname: Qi
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000378:metabolism*; D004742:Enhancer Elements, Genetic; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D061987:Sf9 Cells; D018411:Spodoptera / Q000166:cytology / Q000821:virology; D014764:Viral Proteins / Q000037:antagonists & inhibitors / Q000145:classification / Q000235:genetics / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D034741:RNA, Small Interfering; D014764:Viral Proteins