title: A simple method of labeling amyloid β with quantum dots and ingestion of the labeled amyloid β by astrocytes.
abstract: Steady labeling of amyloid beta (Aβ) is crucial for studying the ingestion and degradation of Aβ by astrocytes and unraveling a relevant regulation mechanism. Quantum dots (QDs) are an optimum labeling reagent for this because of their strong and steady fluorescence properties. In this paper, Aβ was labeled with QDs by a simple mixed incubation strategy, with a QD labeled Aβ complex (QDs-Aβ) being obtained. In the complex, QDs efficiently restrained the formation of β-folding and fibrils of Aβ, while the graininess, dispersivity and fluorescence properties of the QDs hardly changed. The fluorescence microscopy imaging results showed that the astrocytes could ingest the QDs-Aβ. The QDs and Aβ did not separate from each other during the ingestion process, and the Aβ could be degraded subsequently.
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing 100081, People’s Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Xing
authors.initials: X
authors.lastname: Jia
authors.forename: Hong
authors.initials: H
authors.lastname: Qing
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Xie
mesh_terms: D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D045663:Quantum Dots; D051381:Rats; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D016229:Amyloid beta-Peptides