title: Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol rich membranes.
abstract: Arenavirus morphogenesis and budding occurs at cellular plasma membrane; however, the nature of membrane assembly sites remains poorly understood. In this study we examined the effect of different cholesterol-lowering agents on Junín virus (JUNV) multiplication. We found that cholesterol cell depletion reduced JUNV glycoproteins (GPs) membrane expression and virus budding. Analysis of membrane protein insolubility in Triton X-100 suggested that JUNV GPs associate with cholesterol enriched membranes. Rafts dissociation conditions as warm detergent extraction and cholesterol removal by methyl-β-cyclodextrin compound showed to impair GPs cholesterol enriched membrane association. Analysis of GPs transfected cells showed similar results suggesting that membrane raft association is independent of other viral proteins.
authors.affiliation: Laboratorio de Virología, Departamento de Química Biológica, IQUIBICEN, Facultad de Ciencias Exactas y Naturales, UBA, Ciudad Universitaria, Pabellón II, Piso 4, 1428, Buenos Aires, Argentina.
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Cordo
authors.forename: Ayelén
authors.initials: A
authors.lastname: Valko
authors.forename: Guadalupe M
authors.initials: GM
authors.lastname: Martinez
authors.forename: Nélida A
authors.initials: NA
authors.lastname: Candurra
mesh_terms: D000818:Animals; D000924:Anticholesteremic Agents / Q000494:pharmacology; D002462:Cell Membrane / Q000737:chemistry* / Q000821:virology*; D002522:Chlorocebus aethiops; D002784:Cholesterol / Q000737:chemistry*; D004285:Dogs; D018051:Junin virus / Q000502:physiology*; D061985:Madin Darby Canine Kidney Cells; D008562:Membrane Glycoproteins / Q000378:metabolism*; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014779:Virus Replication / Q000187:drug effects / Q000502:physiology*
chemical_list: D000924:Anticholesteremic Agents; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D002784:Cholesterol