title: Oxidative state of the liver of rats with adjuvant-induced arthritis.
abstract: Adjuvant-induced arthritis is an experimental immunopathology in rats that is often used as a model for studying autoimmune chronic inflammation and inflammatory cachexia. In these animals oxidative stress is quite pronounced in the articular inflammation sites. The purpose of this study was to evaluate oxidative stress in the liver of arthritic rats in which morphological and metabolic alterations have been reported to occur. Oxidative injury parameters, levels and production of reactive oxygen species (ROS), and antioxidant parameters were measured in the total liver homogenate and in subcellular fractions, namely cytosol, mitochondria, and peroxisomes. Arthritic rats presented higher levels of ROS than controls in the total homogenate (46% higher) and in all subcellular fractions (51, 38, and 55% higher for mitochondria, peroxisome, and cytosol, respectively). Arthritic rats also presented higher levels of protein carbonyl groups in the total homogenate (75%) and in all subcellular fractions (189, 227, and 260%, respectively, for mitochondria, peroxisomes, and cytosol). The TBARS levels of arthritic rats were more elevated in the total homogenate (36%), mitochondria (20%), and peroxisomes (16%). Arthritic rats also presented higher levels of NO markers in the peroxisomes (112%) and in the cytosol (35%). The catalase activity of all cell compartments was strongly diminished (between 77 and 87%) by arthritis, and glutathione peroxidase activities were diminished in the mitochondria (33.7%) and cytosol (41%). The cytosolic glucose-6-phosphate dehydrogenase activity, on the other hand, was increased (62.9%), the same happening with inducible peroxisomal NO synthase (119.3%). The superoxide dismutase and glutathione reductase activities were not affected. The GSH content was diminished by arthritis in all cellular compartments (50 to 59% diminution). The results reveal that the liver of rats with adjuvant-induced arthritis presents a pronounced oxidative stress and that, in consequence, injury to lipids and proteins is highly significant. The higher ROS content of the liver of arthritic rats seems to be the consequence of both a stimulated pro-oxidant system and a deficient antioxidant defense with a predominance of the latter as indicated by the strongly diminished activities of catalase and glutathione peroxidase.
authors.affiliation: Department of Biochemistry , University of Maringá, 87020900 Maringá, Brazil. jurandircomar@yahoo.com.br
authors.forename: Jurandir Fernando
authors.initials: JF
authors.lastname: Comar
authors.forename: Anacharis
authors.initials: A
authors.lastname: Babeto de Sá-Nakanishi
authors.forename: Andrea Luiza
authors.initials: AL
authors.lastname: de Oliveira
authors.forename: Mariana
authors.initials: M
authors.lastname: Marques Nogueira Wendt
authors.forename: Ciomar Aparecida
authors.initials: CA
authors.lastname: Bersani Amado
authors.forename: Emy Luiza
authors.initials: EL
authors.lastname: Ishii Iwamoto
authors.forename: Rosane Marina
authors.initials: RM
authors.lastname: Peralta
authors.forename: Adelar
authors.initials: A
authors.lastname: Bracht
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D002100:Cachexia / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005620:Freund's Adjuvant / Q000008:administration & dosage*; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress; D020675:Peroxisomes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D005620:Freund's Adjuvant