title: BCAS2 is essential for Drosophila viability and functions in pre-mRNA splicing.
abstract: Here, we show that dBCAS2 (CG4980, human Breast Carcinoma Amplified Sequence 2 ortholog) is essential for the viability of Drosophila melanogaster. We find that ubiquitous or tissue-specific depletion of dBCAS2 leads to larval lethality, wing deformities, impaired splicing, and apoptosis. More importantly, overexpression of hBCAS2 rescues these defects. Furthermore, the C-terminal coiled-coil domain of hBCAS2 binds directly to CDC5L and recruits hPrp19/PLRG1 to form a core complex for splicing in mammalian cells and can partially restore wing damage induced by knocking down dBCAS2 in flies. In summary, Drosophila and human BCAS2 share a similar function in RNA splicing, which affects cell viability.
authors.affiliation: Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei 100, Taiwan.
authors.forename: Po-Han
authors.initials: PH
authors.lastname: Chen
authors.forename: Chia-I
authors.initials: CI
authors.lastname: Lee
authors.forename: Yu-Tzu
authors.initials: YT
authors.lastname: Weng
authors.forename: Woan-Yuh
authors.initials: WY
authors.lastname: Tarn
authors.forename: Yeou-Ping
authors.initials: YP
authors.lastname: Tsao
authors.forename: Ping-Chang
authors.initials: PC
authors.lastname: Kuo
authors.forename: Pang-Hung
authors.initials: PH
authors.lastname: Hsu
authors.forename: Chu-Wei
authors.initials: CW
authors.lastname: Huang
authors.forename: Chiun-Sheng
authors.initials: CS
authors.lastname: Huang
authors.forename: Hsiu-Hsiang
authors.initials: HH
authors.lastname: Lee
authors.forename: June-Tai
authors.initials: JT
authors.lastname: Wu
authors.forename: Show-Li
authors.initials: SL
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D007814:Larva / Q000254:growth & development; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D009928:Organ Specificity; D010641:Phenotype; D011401:Promoter Regions, Genetic; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D012326:RNA Splicing / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins; D014921:Wings, Animal / Q000002:abnormalities* / Q000254:growth & development
chemical_list: C579021:BCAS2 protein, Drosophila; C120624:BCAS2 protein, human; C508357:CDC5L protein, human; D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; C479410:PLRG1 protein, human; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins