title: Existence of glioma stroma mesenchymal stemlike cells in Korean glioma specimens.
abstract: OBJECTIVE
  It was presented that mesenchymal stem cells (MSCs) can be isolated from western glioma specimens. However, whether MSCs exist in glioma specimens of different ethnicities is unknown. To verify the existence of MSCs in an independent cohort, we undertook studies to isolate MSCs from a group of Korean patients. We hypothesized that cells resembling MSCs that were deemed mesenchymal stemlike cells (MSLCs) exist in an independent cohort of Korean gliomas.
  
  
  METHODS
  We cultured fresh glioma specimens using the protocols used for culturing MSCs. The cultured cells were analyzed with fluorescence-activated cell sorting (FACS) for surface markers associated with MSCs. Cultured cells were exposed to mesenchymal differentiation conditions. To presume possible locations of MSLCs in the glioma, sections of glioma were analyzed by immunofluorescent labeling for CD105, CD31, and NG2.
  
  
  RESULTS
  From nine of 31 glioma specimens, we isolated cells resembling MSCs, which were deemed Korean glioma stroma MSLCs (KGS-MSLCs). KGS-MSLCs were spindle shaped and adherent to plastic. KGS-MSLCs had similar surface markers to MSCs (CD105(+), CD90(+), CD73(+), and CD45(-)). KGS-MSLCs were capable of mesenchymal differentiation and might be located around endothelial cells, pericytes, and in a disorganized perivascular area inside glioma stroma.
  
  
  CONCLUSIONS
  We found that cells resembling MSCs indeed exist in an independent cohort of glioma patients, as presented in western populations. We could presume that the possible location of KGS-MSLCs was in perivascular area or in glioma stroma that was a disorganized vascular niche. It might be possible that KGS-MSLCs could be one of constituent of stroma of glioma microenvironment.
authors.affiliation: Department of Neurosurgery, Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors.forename: Young Goo
authors.initials: YG
authors.lastname: Kim
authors.forename: Soyoun
authors.initials: S
authors.lastname: Jeon
authors.forename: Ga-Yeong
authors.initials: GY
authors.lastname: Sin
authors.forename: Jin-Kyoung
authors.initials: JK
authors.lastname: Shim
authors.forename: Bo-Kyung
authors.initials: BK
authors.lastname: Kim
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Shin
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Yong-Min
authors.initials: YM
authors.lastname: Huh
authors.forename: Su-Jae
authors.initials: SJ
authors.lastname: Lee
authors.forename: Eui-Hyun
authors.initials: EH
authors.lastname: Kim
authors.forename: Eun Kyung
authors.initials: EK
authors.lastname: Park
authors.forename: Se-Hoon
authors.initials: SH
authors.lastname: Kim
authors.forename: Jong Hee
authors.initials: JH
authors.lastname: Chang
authors.forename: Dong Seok
authors.initials: DS
authors.lastname: Kim
authors.forename: Sun Ho
authors.initials: SH
authors.lastname: Kim
authors.forename: Yong-Kil
authors.initials: YK
authors.lastname: Hong
authors.forename: Seok-Gu
authors.initials: SG
authors.lastname: Kang
authors.forename: Frederick F
authors.initials: FF
authors.lastname: Lang
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D002469:Cell Separation; D005434:Flow Cytometry; D005910:Glioma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D056910:Republic of Korea
chemical_list: D015703:Antigens, CD