title: Detailed modes of action and biochemical characterization of endo-arabinanase from Bacillus licheniformis DSM13.
abstract: An endo-arabinanase (BLABNase) gene from Bacillus licheniformis DSM13 was cloned and expressed in Escherichia                 coli, and the biochemical properties of its encoded enzyme were characterized. The BLABNase gene consists of a single open reading frame of 987 nucleotides that encodes 328 amino acids with a predicted molecular mass of about 36 kDa. BLABNase exhibited the highest activity against debranched α-(1,5)-arabinan in 50 mM sodium acetate buffer (pH 6.0) at 55°C. Enzymatic characterization revealed that BLABNase hydrolyzes debranched or linear arabinans with a much higher activity than branched arabinan from sugar beet. Enzymatic hydrolysis pattern analyses demonstrated BLABNase to be a typical endo-(1,5)-α-S-arabinanase (EC 3.2.1.99) that randomly cleaves the internal α-(1,5)-linked L-arabinofuranosyl residues of a branchless arabinan backbone to release arabinotriose mainly, although a small amount of arabino-oligosaccharide intermediates is also liberated. Our results indicated that BLABNase acts preferentially along with the oligosaccharides longer than arabinopentaose, thus enabling the enzymatic production of various arabino-oligosaccharides.
authors.affiliation: Department of Food Science and Technology, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: Jung-Mi
authors.initials: JM
authors.lastname: Park
authors.forename: Myoung-Uoon
authors.initials: MU
authors.lastname: Jang
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Kang
authors.forename: Min-Jeong
authors.initials: MJ
authors.lastname: Kim
authors.forename: So-Won
authors.initials: SW
authors.lastname: Lee
authors.forename: Yeong Bok
authors.initials: YB
authors.lastname: Song
authors.forename: Chul-Soo
authors.initials: CS
authors.lastname: Shin
authors.forename: Nam Soo
authors.initials: NS
authors.lastname: Han
authors.forename: Tae-Jip
authors.initials: TJ
authors.lastname: Kim
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D004789:Enzyme Activation; D004795:Enzyme Stability; D015870:Gene Expression; D023061:Gene Order; D006026:Glycoside Hydrolases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D011994:Recombinant Proteins; C074451:Endo-arabinase; D006026:Glycoside Hydrolases