title: Chromogenic substrate from 4-nitro-1-naphthol for hydrolytic enzyme of neutral or slightly acidic optimum pH: 4-nitro-1-naphthyl-β-D-galactopyranoside as an example.
abstract: At pH from 5.5 to 7.6, absorptivity of 4-nitro-1-naphthol at 450 nm is over 2.1-fold of that of para-nitrophenol at 405 nm and over 9.6-fold of that of ortho-nitrophenol at 415 nm. On 4-nitro-1-naphthyl-β-D-galactopyranoside at pH 7.4, catalytic efficiency of Escherichia coli β-D-galactosidase is 3-fold of that on para-nitrophenyl-β-D-galactopyranoside and about 40% of that on ortho-nitrophenyl-β-D-galactopyranoside, and produces a lower quantification limit of penicillin G by enzyme-linked-immunoabsorbent-assay. Hence, 4-nitro-1-naphthol is favorable to prepare chromogenic substrates of hydrolytic enzymes of neutral or slightly acidic optimum pH.
authors.affiliation: Unit for Analytical Probes and Protein Biotechnology, Key Laboratory of Medical Laboratory Diagnostics of the Education Ministry, College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Jizheng
authors.initials: J
authors.lastname: Dang
authors.forename: Hongbo
authors.initials: H
authors.lastname: Liu
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Yang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yanling
authors.initials: Y
authors.lastname: Xie
authors.forename: Yuanli
authors.initials: Y
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Pu
authors.forename: Juan
authors.initials: J
authors.lastname: Liao
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Yuan
authors.forename: Fei
authors.initials: F
authors.lastname: Liao
mesh_terms: D002863:Chromogenic Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005960:Glucosides / Q000138:chemical synthesis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D015394:Molecular Structure; D009281:Naphthalenes / Q000138:chemical synthesis* / Q000737:chemistry; D009284:Naphthols / Q000737:chemistry*; D009596:Nitrophenols / Q000737:chemistry*; D010400:Penicillin G / Q000737:chemistry; D001616:beta-Galactosidase / Q000737:chemistry
chemical_list: D002863:Chromogenic Compounds; D005960:Glucosides; D009281:Naphthalenes; D009284:Naphthols; D009596:Nitrophenols; C029350:1-naphthol; D001616:beta-Galactosidase; D010400:Penicillin G