title: Distinct tissue expression profiles of chicken Lpin1-α/β isoforms and the effect of the variation on muscle fiber traits.
abstract: Here we cloned chicken Lpin1-β, conducted the temporal and spatial expression pattern analysis of chicken Lpin1 isoforms by real-time PCR, and studied the 5' flanking region variation and the potential effect. It was found that chicken Lpin1-α and Lpin1-β exhibited distinct tissue expression profiling, with prominent expression in the ovary and muscle tissues respectively. Chicken Lpin1 presented a tissue-specific temporal expression pattern in postnatal development (0-16 weeks). Energy restriction significantly elevated the mRNA level of total Lpin1 by increasing the expression of Lpin1-α and Lpin1-β in a nearly same magnitude. Eight variants/four haplotypes among six breeds were detected from the 5' flanking region of chicken Lpin1, one multiple-nucleotide length polymorphism (g.258M>N) was found and predicted causing the change of 31 transcription factor binding sites including MyoD et al. Both g.258M>N and g. 65C>T variants showed significant association with muscle fiber traits, which suggested one novel role of Lpin1 on muscle fiber development.
authors.affiliation: College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, Henan, China.
authors.forename: Suya
authors.initials: S
authors.lastname: Li
authors.forename: Wen
authors.initials: W
authors.lastname: Chen
authors.forename: Xiangtao
authors.initials: X
authors.lastname: Kang
authors.forename: Ruili
authors.initials: R
authors.lastname: Han
authors.forename: Guirong
authors.initials: G
authors.lastname: Sun
authors.forename: Yanqun
authors.initials: Y
authors.lastname: Huang
mesh_terms: D024506:5' Flanking Region; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D031204:Caloric Restriction; D002645:Chickens / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D006239:Haplotypes; D050356:Lipid Metabolism / Q000235:genetics; D008099:Liver / Q000201:enzymology / Q000254:growth & development; D008969:Molecular Sequence Data; D018485:Muscle Fibers, Skeletal / Q000201:enzymology*; D018482:Muscle, Skeletal / Q000201:enzymology / Q000254:growth & development; D009928:Organ Specificity; D010641:Phenotype; D010711:Phosphatidate Phosphatase / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D011110:Polymorphism, Genetic; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D020033:Protein Isoforms; D010711:Phosphatidate Phosphatase