title: Comparison of chromatographic techniques for diastereomer separation of a diverse set of drug-like compounds.
abstract: Preparative supercritical fluid chromatography (SFC) has proven value for isocratic bulk separation of both diastereomers and enantiomers. With the recent availability of SFC equipment suitable for rapid gradient separation [Ebinger et al. JALA (2011) 16 (3) 241], we have become interested in comparing the effectiveness of traditional reverse phase high performance liquid chromatography (HPLC) with SFC using non-chiral columns for the separation of diastereomeric mixtures. The success rates for separation of a diverse set of 258 synthetic diastereomer pairs were used to determine the relative utility of the aforementioned two techniques. Our results suggest that gradient non-chiral SFC was more successful than the traditional non-chiral HPLC technique for diastereomer separations of the diverse sample set of 258 drug-like compounds.
authors.affiliation: Bristol-Myers Squibb Company, 5 Research Parkway, Wallingford, CT 06492, USA. katalin.ebinger@bms.com
authors.forename: Katalin
authors.initials: K
authors.lastname: Ebinger
authors.forename: Harold N
authors.initials: HN
authors.lastname: Weller
mesh_terms: D002845:Chromatography / Q000379:methods*; D006860:Hydrogen Bonding; D008970:Molecular Weight; D004364:Pharmaceutical Preparations / Q000737:chemistry* / Q000302:isolation & purification*; D013237:Stereoisomerism
chemical_list: D004364:Pharmaceutical Preparations