title: Human adipose stromal vascular cell delivery in a fibrin spray.
abstract: OBJECTIVE
  Adipose tissue represents a practical source of autologous mesenchymal stromal cells (MSCs) and vascular-endothelial progenitor cells, available for regenerative therapy without in vitro expansion. One of the problems confronting the therapeutic application of such cells is how to immobilize them at the wound site. We evaluated in vitro the growth and differentiation of human adipose stromal vascular fraction (SVF) cells after delivery through the use of a fibrin spray system.
  
  
  METHODS
  SVF cells were harvested from four human adult patients undergoing elective abdominoplasty, through the use of the LipiVage system. After collagenase digestion, mesenchymal and endothelial progenitor cells (pericytes, supra-adventitial stromal cells, endothelial progenitors) were quantified by flow cytometry before culture. SVF cells were applied to culture vessels by means of the Tisseel fibrin spray system. SVF cell growth and differentiation were documented by immunofluorescence staining and photomicrography.
  
  
  RESULTS
  SVF cells remained viable after application and were expanded up to 3 weeks, when they reached confluence and adipogenic differentiation. Under angiogenic conditions, SVF cells formed endothelial (vWF+, CD31+ and CD34+) tubules surrounded by CD146+ and α-smooth muscle actin+ perivascular/stromal cells.
  
  
  CONCLUSIONS
  Human adipose tissue is a rich source of autologous stem cells, which are readily available for regenerative applications such as wound healing, without in vitro expansion. Our results indicate that mesenchymal and endothelial progenitor cells, prepared in a closed system from unpassaged lipoaspirate samples, retain their growth and differentiation capacity when applied and immobilized on a substrate using a clinically approved fibrin sealant spray system.
authors.affiliation: Department of Cardiothoracic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Zimmerlin
authors.forename: J Peter
authors.initials: JP
authors.lastname: Rubin
authors.forename: Melanie E
authors.initials: ME
authors.lastname: Pfeifer
authors.forename: Linda R
authors.initials: LR
authors.lastname: Moore
authors.forename: Vera S
authors.initials: VS
authors.lastname: Donnenberg
authors.forename: Albert D
authors.initials: AD
authors.lastname: Donnenberg
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D005337:Fibrin / Q000737:chemistry*; D005434:Flow Cytometry; D006801:Humans; D020286:Pericytes / Q000166:cytology; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells; D014945:Wound Healing / Q000502:physiology
chemical_list: D005337:Fibrin