title: Enhanced adsorption capacity of cryogel bed by incorporating polymeric resin particles.
abstract: Novel composite cryogel monoliths were fabricated by incorporating polymeric resin particles and grafting anion-exchange groups on the pore wall surfaces. The embedded resin particles in different size distributions were prepared by grinding poly(glycidyl methacrylate-ethylene glycol dimethacrylate) monoliths. Observations by scanning electron microscopy clearly indicated that the composite cryogel had interconnected large pores (10-100 μm in width), similar to normal (pure) cryogel. However, the composite material had very rough pore walls, which provided larger surface area for protein adsorption. As a result, the dynamic binding capacity of bovine serum albumin (BSA) on the composite cryogel bed reached 6 mg/mL bed (flow velocity, 5 cm/min), which was 2.8 times higher than a cryogel bed without embedding the ground resin particles. The capacity value was also much higher than the BSA capacities of cryogel beds reported in literature (1-4 mg/mL). Though the capacity decreased by about 1mg/mL with the increase of flow rate from 0.5 to 5 cm/min, it then kept almost unchanged till a flow rate up to 15 cm/min. The height equivalent to a theoretical plate of the composite bed was in the range of 1.1-1.4mm, and kept nearly constant in a flow rate range of 5-20 cm/min. The results indicated that the composite cryogel bed offered a large improvement in protein adsorption capacity and was suitable for high-speed protein chromatography.
authors.affiliation: Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Wang
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Dong
authors.forename: Zhongyi
authors.initials: Z
authors.lastname: Jiang
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D002845:Chromatography; D059985:Cryogels / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry*; D012212:Rheology; D012710:Serum Albumin, Bovine / Q000378:metabolism
chemical_list: D059985:Cryogels; D011108:Polymers; D012117:Resins, Synthetic; D012710:Serum Albumin, Bovine