title: [Study on urine biomarkers in 1,3-butadiene exposed workers].
abstract: OBJECTIVE
  To discuss the urine biomarkers in 1,3-butadiene exposed workers, and to provide basement for establishing biological limit value.
  
  
  METHODS
  44 BD exposed workers as exposure group and 25 BD non-exposed people as control group including 12 workers in boiler workshop in the same factory and 13 people in one public institute, we collected their in-end-of shift urine, then detected urine BD-derived mercapturic metabolites [3,4-dihydroxybutyl mercapturic acid (DHBMA),1- and 2-monohydroxy-3-butenyl mercapturic acid (MHBMA)] concentrations using UPLC-MS/MS method. Meanwhile, we detected air BD concentration with GC-FID in the workplace, and compared their relationship.
  
  
  RESULTS
  lgDHBMA and lg (MHBMA + DHBMA) levels in exposed group (lgDHBMA: 2.51 ± 0.44) µg/L, lg [MHBMA + DHBMA: (2.68 ± 0.27) µg/L] were higher than which in control group (lgDHBMA: (2.20 ± 0.25) µg/L, lg(MHBMA + DHBMA: (2.49 ± 0.34) µg/L), and the differences were significant (P < 0.01). Urine DHBMA was obviously influenced by air BD concentrations (r = 0.539, P = 0.001). The equation of Multiple Regression Analysis was y = 2.417 + 0.520x (x represents air BD dose, and represents urinary DHBMA level). Adjusted R(2) of this model was 0.262. Urinary MHBMA was not affected by smoking, alcohol and years of works.
  
  
  CONCLUSIONS
  Urine metabolite DHBMA in BD-exposed workers might be major biological exposure indice.
authors.affiliation: Fudan University, Public Health School, Shanghai 200032, China.
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Cheng
authors.forename: Yan-ni
authors.initials: YN
authors.lastname: Jiao
authors.forename: Jin-dong
authors.initials: JD
authors.lastname: Chen
authors.forename: Bao-de
authors.initials: BD
authors.lastname: Shan
authors.forename: Zhao-lin
authors.initials: ZL
authors.lastname: Xia
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000652:urine*; D002070:Butadienes; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects*; D011795:Surveys and Questionnaires
chemical_list: D015415:Biomarkers; D002070:Butadienes; C031763:1,3-butadiene