title: Testing chemical agents with the cytokinesis-block micronucleus cytome assay.
abstract: In order to evaluate the applicability of the           cytokinesis-block micronucleus cytome assay in routine           mutagenicity testing we investigated with this           method different chemicals having different mechanisms           of action: non-mutagens, direct-acting basealtering           mutagens, direct-acting cross-linking mutagens,           clastogens including a radiomimetic chemical,           indirect-acting spindle poisons and indirect-acting           enzyme inhibitors. We looked at the presence of micronuclei           as biomarkers for either the loss of chromosome           fragments (clastogen) or the loss of a whole           chromosome (aneugen), nucleoplasmic bridges as           biomarkers for complex rearrangements (e.g., dicentric           chromosomes) and nuclear buds as biomarkers           for gene amplification. The cytome assay proved to           be a suitable tool to investigate genetic effects of environmental           agents and to provide insight into their           working mechanisms as all chemicals tested showed           the expected response.
authors.affiliation: Scientific Institute of Public Health, O.D. Public Health and Surveillance, Laboratory of Toxicology, Brussels, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Maes
authors.forename: R
authors.initials: R
authors.lastname: Anthonissen
authors.forename: L
authors.initials: L
authors.lastname: Verschaeve
mesh_terms: D002460:Cell Line; D048749:Cytokinesis / Q000187:drug effects*; D006801:Humans; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D015162:Micronucleus Tests / Q000379:methods*; D009153:Mutagens / Q000633:toxicity*
chemical_list: D009153:Mutagens