title: The protective effects of jatrorrhizine on β-amyloid (25-35)-induced neurotoxicity in rat cortical neurons.
abstract: Alzheimer's disease (AD) is a neurodegenerative disorder characterized by selective neuronal loss, amyloid plaques and neurofibrillary tangles. Oxidative stress may play an important role in the pathogenesis of AD which is associated with the accumulation of β-amyloid (Aβ). Jatrorrhizine (JAT) is a novel tetrahydroisoquinoline alkaloid originally extracted from the Chinese herb coptidis rhizome. Our previous studies showed that JAT protected neuronallike cells against H₂O₂ -induced toxicity. In this study, we investigated the protective effects of JAT against Aβ₂₅₋₃₅- induced cell death in rat cortical neurons. When the cortical neurons were exposed to 25 μM Aβ₂₅₋₃₅ for 24h, there was a significant reduction in cell viability and activities of SOD and GSH-Px. It also increased the production of malondialdehyde (MDA) and ROS but reduced MMP. Pretreatment of the cortical neurons with various concentrations of JAT (1-10 μM) attenuated Aβ₂₅₋₃₅-induced neurotoxicity markedly. JAT was also showed to suppress the activation of caspase-3 induced by Aβ₂₅₋₃₅ and prevented the cytochrome c transporting into the cytosol. These results indicate that JAT demonstrates the neuroprotective effects against Aβ₂₅₋₃₅-induced injury via its antioxidative potential, which may provide a therapeutical potential to AD.
authors.affiliation: Department of Anatomy and Neurobiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, 510080, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Luo
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.forename: Yan
authors.initials: Y
authors.lastname: Kong
authors.forename: Sheng
authors.initials: S
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: He
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.forename: Hua-Qiao
authors.initials: HQ
authors.lastname: Wang
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D000831:Animals, Newborn; D001599:Berberine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D010446:Peptide Fragments / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome
chemical_list: D016229:Amyloid beta-Peptides; D018696:Neuroprotective Agents; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35); C055785:jatrorrhizine; D001599:Berberine