title: Allografts with autogenous platelet-rich plasma for tibial defect reconstruction: a rabbit study.
abstract: OBJECTIVE
  To evaluate the effect of autogenous platelet-rich plasma (PRP) for fresh-frozen allografts in tibial defect reconstruction in rabbits.
  
  
  METHODS
  40 adult New Zealand white rabbits underwent tibial defect reconstruction with autografts (n=12), allografts without PRP (n=12), or allografts with PRP (n=12) and were observed for 12, 16, and 24 weeks (4 for each period). Tibias of the remaining 4 rabbits were used as donor allografts, and the remaining allografts were procured from recipient rabbits. A 1.5- cm cortical segment of the tibia was osteotomised, and then fixed with a 9-hole mini-compression plate and 2 cerclage wires. Allografts were stripped off the periosteum and soft tissues and medullary contents, and then stored in a freezer at -80 ºC. All allografts were deep frozen for at least 4 weeks before transplantation. 7 ml of whole blood was drawn to prepare 1 ml of PRP. The PRP was then mixed with 1.0 ml of human thrombin to form a platelet gel. The PRP gel was then packed into the medullary canal of the allograft and applied on the cortical surface before tibial defect reconstruction. Rabbits were sacrificed at 12, 16, and 24 weeks. The specimens were assessed for bone union at host-graft junctions and for bone resorption, new bone formation, callus encasement, and viable osteocyte counts.
  
  
  RESULTS
  There were 4 specimens in each group at each observation period. Osteoid bridging the gap at host-graft junctions was noted in all specimens in the autograft and allograft-with-PRP groups at week 12 and in the allograft-without-PRP group at week 24. Bone union in allografts without PRP was delayed. All indices for biological incorporation (resorption index, new bone formation index, callus encasement index, and viable osteocyte count) were significantly greater in the autograft than allograft-without-PRP groups, except for the resorption index at week 24, whereas the differences were not significant between the autograft and allograft-with-PRP groups. The differences between the 2 allograft groups were usually not significant, except for the resorption index.
  
  
  CONCLUSIONS
  PRP-augmented allografts behaved similarly to autografts for tibial defect reconstruction in rabbits. PRP increased bone union and bone resorption.
authors.affiliation: Department of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Aziz
authors.initials: A
authors.lastname: Nather
authors.forename: Keng Lin
authors.initials: KL
authors.lastname: Wong
authors.forename: Vikram
authors.initials: V
authors.lastname: David
authors.forename: Barry P
authors.initials: BP
authors.lastname: Pereira
mesh_terms: D064591:Allografts; D000818:Animals; D064592:Autografts; D002146:Bony Callus / Q000473:pathology; D008297:Male; D053657:Platelet-Rich Plasma; D011817:Rabbits; D019651:Plastic Surgery Procedures; D013977:Tibia / Q000601:surgery / Q000637:transplantation*; D014947:Wounds and Injuries / Q000601:surgery*