title: Performance of single particle fritted capillary columns in electrochromatography.
abstract: Development of capillary electrochromatography (CEC) largely depends on column technology. The past ten years or so have seen a great number of CEC works performed on monolithic columns, due to simplicity and robustness in column fabrication. Monolithic columns eliminate the issue of column fritting, which conventionally made particle-packed capillary columns fragile and introduced nonuniformity to the chromatographic bed. The particulate packing material, however, is still a popular type of stationary phase widely used in CEC, as the rich library of HPLC packing material provides a wide range of choices for chromatographic separations performed in electrodriven mode. In this study, we investigated a purely physical fritting method, single particle fritting technology, to immobilize particulate chromatographic material inside capillary tube in a sinter-free manner to produce robust capillary columns. Single particle fritted columns present significantly improved column-to-column reproducibility (n=10) in peak efficiency, retention factor, peak area and asymmetry (%RSD=5.4, 7.7, 6.2 and 6.1, respectively, at 26 kV), enabling their practical application in high throughput parallel analysis using multiple columns.
authors.affiliation: Department of Chemistry and the Key Laboratory of Analytical Sciences, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China. bozhang@xmu.edu.cn
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: Lijun
authors.initials: L
authors.lastname: Yang
authors.forename: Qiuquan
authors.initials: Q
authors.lastname: Wang
mesh_terms: D053801:Capillary Electrochromatography / Q000295:instrumentation* / Q000379:methods* / Q000592:standards; D008855:Microscopy, Electron, Scanning; D052638:Particulate Matter / Q000737:chemistry*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry
chemical_list: D052638:Particulate Matter; D011084:Polycyclic Aromatic Hydrocarbons; D012997:Solvents