title: A validated UHPLC-diode array detector method for the bioanalysis of atypical antipsychotics in whole blood, urine and cerebrospinal fluid following SPE.
abstract: BACKGROUND
  Risperidone, quetiapine, amisulpride and aripiprazole are atypical antipsychotics increasingly prescribed in Greece.
  
  
  RESULTS
  We have developed and validated a UHPLC-diode array detector method for the determination of these drugs in whole blood, urine and cerebrospinal fluid, following SPE. The separation was achieved within 7 min. Validation was performed in terms of linearity, selectivity, accuracy, precision and stability. Following validation, the method was applied to post-mortem blood, urine and cerebrospinal fluid samples.
  
  
  CONCLUSIONS
  The method proved fast and simple for the determination of four atypical antipsychotic drugs in whole blood, urine and cerebrospinal fluid. To the best of our knowledge, this is the only available method for the simultaneous determination of these drugs in cerebrospinal fluid.
authors.affiliation: Laboratory of Forensic Medicine & Toxicology, School of Medicine, Aristotle University of Thessaloniki, Greece.
authors.forename: Leda
authors.initials: L
authors.lastname: Kovatsi
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Redifis
authors.forename: Katia
authors.initials: K
authors.lastname: Mihailidou
authors.forename: Pavlos
authors.initials: P
authors.lastname: Pavlidis
authors.forename: Victoria
authors.initials: V
authors.lastname: Samanidou
mesh_terms: D014150:Antipsychotic Agents / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid / Q000652:urine; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D014150:Antipsychotic Agents