title: Integration of datasets from different analytical techniques to assess the impact of nutrition on human metabolome.
abstract: Bacteria colonizing the human intestinal tract exhibit a high phylogenetic diversity that reflects their immense metabolic potentials. The catalytic activity of gut microbes has an important impact on gastrointestinal (GI) functions and host health. The microbial conversion of carbohydrates and other food components leads to the formation of a large number of compounds that affect the host metabolome and have beneficial or adverse effects on human health. Metabolomics is a metabolic-biology system approach focused on the metabolic responses understanding of living systems to physio-pathological stimuli by using multivariate statistical data on human body fluids obtained by different instrumental techniques. A metabolomic approach based on an analytical platform could be able to separate, detect, characterize and quantify a wide range of metabolites and its metabolic pathways. This approach has been recently applied to study the metabolic changes triggered in the gut microbiota by specific diet components and diet variations, specific diseases, probiotic and synbiotic food intake. This review describes the metabolomic data obtained by analyzing human fluids by using different techniques and particularly Gas Chromatography Mass Spectrometry Solid-phase Micro Extraction (GC-MS/SPME), Proton Nuclear Magnetic Resonance ((1)H-NMR) Spectroscopy and Fourier Transform Infrared (FTIR) Spectroscopy. This instrumental approach has a good potential in the identification and detection of specific food intake and diseases biomarkers.
authors.affiliation: Interdipartimental Centre for Industrial Research-CIRI-AGRIFOOD, Alma Mater Studiorum, University of Bologna Bologna, Italy ; Parasitology Unit, Department of Laboratories, Bambino Gesù Children's Hospital, IRCCS Rome, Italy.
authors.forename: Pamela
authors.initials: P
authors.lastname: Vernocchi
authors.forename: Lucia
authors.initials: L
authors.lastname: Vannini
authors.forename: Davide
authors.initials: D
authors.lastname: Gottardi
authors.forename: Federica
authors.initials: F
authors.lastname: Del Chierico
authors.forename: Diana I
authors.initials: DI
authors.lastname: Serrazanetti
authors.forename: Maurice
authors.initials: M
authors.lastname: Ndagijimana
authors.forename: Maria E
authors.initials: ME
authors.lastname: Guerzoni
mesh_terms: D001826:Body Fluids / Q000737:chemistry; D004032:Diet / Q000379:methods*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D055442:Metabolome; D015596:Nutrition Assessment; D017550:Spectroscopy, Fourier Transform Infrared