title: Staining of MUC1 in ovarian cancer tissues with PankoMab-GEX detecting the tumour-associated epitope, TA-MUC1, as compared to antibodies HMFG-1 and 115D8.
abstract: PankoMab-GEX is a novel humanized and glycooptimized antibody, which recognizes a novel specific tumour epitope of MUC1 (TA-MUC1). The aim of this study was to evaluate PankoMab-GEX binding to a variety of ovarian cancer specimens (n=156) and to normal ovarian tissue. In addition, PankoMab-GEX staining was compared to that of the well-known anti-MUC1 antibodies HMFG-1 and 115D8. PankoMab-GEX showed positive reactivity in serous (100% of cases, mean IRS 8.23), endometrioid (95% of cases, mean IRS 6.40), mucinous (58% of cases, mean IRS 4.17), and clear cell (92% of cases, mean IRS 7.58) carcinomas. In contrast to HMFG-1, healthy ovarian tissue was not recognized by PankoMab-GEX. Staining with antibody 115D8 was increased with staging. Cytoplasmic PankoMab-GEX staining increased with tumour grade, but no correlation was found with staging. Univariate Kaplan-Meier analysis revealed a tendency of reduced survival of patients with high expression of TA-MUC1. The findings are encouraging with respect to a potential use of PankoMab-GEX as a new therapeutic antibody for the treatment of ovarian cancer patients.
authors.affiliation: Department of Obstetrics and Gynecology, Ludwig-Maximilians-University Munich, Munich, Germany.
authors.forename: Darius
authors.initials: D
authors.lastname: Dian
authors.forename: Miriam
authors.initials: M
authors.lastname: Lenhard
authors.forename: Doris
authors.initials: D
authors.lastname: Mayr
authors.forename: Sabine
authors.initials: S
authors.lastname: Heublein
authors.forename: Uwe
authors.initials: U
authors.lastname: Karsten
authors.forename: Steffen
authors.initials: S
authors.lastname: Goletz
authors.forename: Christina
authors.initials: C
authors.lastname: Kuhn
authors.forename: Irmi
authors.initials: I
authors.lastname: Wiest
authors.forename: Klaus
authors.initials: K
authors.lastname: Friese
authors.forename: Tobias
authors.initials: T
authors.lastname: Weissenbacher
authors.forename: Udo
authors.initials: U
authors.lastname: Jeschke
mesh_terms: D018262:Adenocarcinoma, Clear Cell / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism*; D061067:Antibodies, Monoclonal, Humanized / Q000276:immunology / Q000378:metabolism; D058846:Antibodies, Monoclonal, Murine-Derived / Q000276:immunology / Q000378:metabolism; D000918:Antibody Specificity; D014408:Biomarkers, Tumor / Q000276:immunology / Q000378:metabolism*; D018269:Carcinoma, Endometrioid / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D000939:Epitopes / Q000276:immunology / Q000378:metabolism*; D005260:Female; D006652:Histological Techniques / Q000379:methods; D006801:Humans; D018396:Mucin-1 / Q000276:immunology / Q000378:metabolism*; D018297:Neoplasms, Cystic, Mucinous, and Serous / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D010051:Ovarian Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D010053:Ovary / Q000378:metabolism / Q000473:pathology; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D058846:Antibodies, Monoclonal, Murine-Derived; D014408:Biomarkers, Tumor; D000939:Epitopes; D018396:Mucin-1; C581698:PankoMab-GEX; C469215:pemtumomab