title: Pineapple translation factor SUI1 and ribosomal protein L36 promoters drive constitutive transgene expression patterns in Arabidopsis thaliana.
abstract: The availability of a variety of promoter sequences is necessary for the genetic engineering of plants, in basic research studies and for the development of transgenic crops. In this study, the promoter and 5' untranslated regions of the evolutionally conserved protein translation factor SUI1 gene and ribosomal protein L36 gene were isolated from pineapple and sequenced. Each promoter was translationally fused to the GUS reporter gene and transformed into the heterologous plant system Arabidopsis thaliana. Both the pineapple SUI1 and L36 promoters drove GUS expression in all tissues of Arabidopsis at levels comparable to the CaMV35S promoter. Transient assays determined that the pineapple SUI1 promoter also drove GUS expression in a variety of climacteric and non-climacteric fruit species. Thus the pineapple SUI1 and L36 promoters demonstrate the potential for using translation factor and ribosomal protein genes as a source of promoter sequences that can drive constitutive transgene expression patterns.
authors.affiliation: University of Queensland, Brisbane, 4072, Australia.
authors.forename: Jonni
authors.initials: J
authors.lastname: Koia
authors.forename: Richard
authors.initials: R
authors.lastname: Moyle
authors.forename: Caroline
authors.initials: C
authors.lastname: Hendry
authors.forename: Lionel
authors.initials: L
authors.lastname: Lim
authors.forename: José Ramón
authors.initials: JR
authors.lastname: Botella
mesh_terms: D031230:Ananas / Q000235:genetics* / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D039642:Eukaryotic Initiation Factors / Q000235:genetics / Q000378:metabolism; D005470:Fluorometry; D005638:Fruit / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism; D019076:Transgenes / Q000235:genetics*
chemical_list: D039642:Eukaryotic Initiation Factors; D010940:Plant Proteins; D012269:Ribosomal Proteins; D005966:Glucuronidase