title: Distinct transcriptional signatures of bone marrow-derived C57BL/6 and DBA/2 dendritic leucocytes hosting live Leishmania amazonensis amastigotes.
abstract: OBJECTIVE
  The inoculation of a low number (10(4)) of L. amazonensis metacyclic promastigotes into the dermis of C57BL/6 and DBA/2 mouse ear pinna results in distinct outcome as assessed by the parasite load values and ear pinna macroscopic features monitored from days 4 to 22-phase 1 and from days 22 to 80/100-phase 2. While in C57BL/6 mice, the amastigote population size was increasing progressively, in DBA/2 mice, it was rapidly controlled. This latter rapid control did not prevent intracellular amastigotes to persist in the ear pinna and in the ear-draining lymph node/ear-DLN. The objectives of the present analysis was to compare the dendritic leukocytes-dependant immune processes that could account for the distinct outcome during the phase 1, namely, when phagocytic dendritic leucocytes of C57BL/6 and DBA/2 mice have been subverted as live amastigotes-hosting cells.
  
  
  RESULTS
  Being aware of the very low frequency of the tissues' dendritic leucocytes/DLs, bone marrow-derived C57BL/6 and DBA/2 DLs were first generated and exposed or not to live DsRed2 expressing L. amazonensis amastigotes. Once sorted from the four bone marrow cultures, the DLs were compared by Affymetrix-based transcriptomic analyses and flow cytometry. C57BL/6 and DBA/2 DLs cells hosting live L. amazonensis amastigotes do display distinct transcriptional signatures and markers that could contribute to the distinct features observed in C57BL/6 versus DBA/2 ear pinna and in the ear pinna-DLNs during the first phase post L. amazonensis inoculation.
  
  
  CONCLUSIONS
  The distinct features captured in vitro from homogenous populations of C57BL/6 and DBA/2 DLs hosting live amastigotes do offer solid resources for further comparing, in vivo, in biologically sound conditions, functions that range from leukocyte mobilization within the ear pinna, the distinct emigration from the ear pinna to the DLN of live amastigotes-hosting DLs, and their unique signalling functions to either naive or primed T lymphocytes.
authors.affiliation: Département de Parasitologie et Mycologie, Laboratoire Immunophysiologie et Parasitisme, Institut Pasteur, Paris, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Giraud
authors.forename: Hervé
authors.initials: H
authors.lastname: Lecoeur
authors.forename: Guillaume
authors.initials: G
authors.lastname: Soubigou
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Coppée
authors.forename: Geneviève
authors.initials: G
authors.lastname: Milon
authors.forename: Eric
authors.initials: E
authors.lastname: Prina
authors.forename: Thierry
authors.initials: T
authors.lastname: Lang
mesh_terms: D000818:Animals; D003713:Dendritic Cells / Q000469:parasitology*; D005260:Female; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D007894:Leishmania mexicana / Q000254:growth & development / Q000472:pathogenicity*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA