title: Flow-through comb electroporation device for delivery of macromolecules.
abstract: We present a microfluidic electroporation device with a comb electrode layout fabricated in polydimethylsiloxane (PMDS) and glass. Characterization experiments with HeLa cells and fluorescent dextran show efficient delivery (∼95%) with low toxicity (cell viability ∼85%) as well as rapid pore closure after electroporation. The activity of delivered molecules is also verified by silencing RNA (siRNA) studies that demonstrate gene knockdown in GFP expressing cells. This simple, scalable approach to microfluidic, flow-through electroporation could facilitate the integration of electroporation modules within cell analysis devices that perform multiple operations.
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue Cambridge, Massachusetts 02139, United States.
authors.forename: Andrea
authors.initials: A
authors.lastname: Adamo
authors.forename: Alessandro
authors.initials: A
authors.lastname: Arione
authors.forename: Armon
authors.initials: A
authors.lastname: Sharei
authors.forename: Klavs F
authors.initials: KF
authors.lastname: Jensen
mesh_terms: D000546:Amanitins / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism; D018274:Electroporation / Q000295:instrumentation / Q000379:methods*; D018014:Gene Transfer Techniques / Q000295:instrumentation; D006367:HeLa Cells; D006801:Humans; D046911:Macromolecular Substances / Q000008:administration & dosage / Q000378:metabolism*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism
chemical_list: D000546:Amanitins; D046911:Macromolecular Substances; D034741:RNA, Small Interfering; C018358:phallotoxin