title: Taxonomy and chemical characterization of new antibiotics produced by Saccharothrix SA198 isolated from a Saharan soil.
abstract: Actinomycete strain SA198, isolated from a Saharan soil sample of Algeria, exhibited antimicrobial activity against Gram-positive and Gram-negative bacteria, and phytopathogenic and toxinogenic fungi. The morphological and chemotaxonomic characteristics of the strain were consistent with those of the genus Saccharothrix. Analysis of the 16S rRNA gene sequence of strain SA198 showed a similarity level ranging between 97.2 and 98.8% within Saccharothrix species, S. australiensis being the most closely related. Two new active products were isolated by reverse HPLC using a C18 column. The ultraviolet-visible (UV-VIS), infrared (IR), mass, and (1)H and (14)C nuclear magnetic resonance (NMR) spectra showed that these products were new bioactive compounds. The minimum inhibitory concentrations of these antibiotics showed a strong activity against fungi and moderate activities against Gram-positive and Gram-negative bacteria.
authors.affiliation: Laboratoire de Biologie des Systèmes Microbiens, Ecole Normale Supérieure de Kouba, Alger, Algeria.
authors.forename: D
authors.initials: D
authors.lastname: Boubetra
authors.forename: N
authors.initials: N
authors.lastname: Sabaou
authors.forename: A
authors.initials: A
authors.lastname: Zitouni
authors.forename: C
authors.initials: C
authors.lastname: Bijani
authors.forename: A
authors.initials: A
authors.lastname: Lebrihi
authors.forename: F
authors.initials: F
authors.lastname: Mathieu
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D000352:Africa, Northern; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001419:Bacteria / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010802:Phylogeny; D012988:Soil Microbiology
chemical_list: D000900:Anti-Bacterial Agents