title: Natural IgG antibody with anti-β-galactosyl specificity suppressed hepatoma cell invasion in culture.
abstract: The effect of natural IgG antibody recognizing β-galactosyl epitope on hepatoma cell invasion was investigated. Anti-β-galactosyl antibody dose-dependently suppressed hepatoma invasion underneath primarily cultured mesothelial cells monolayer without affecting the proliferation, to the same extent as natural IgG antibody with anti-α-galactosyl specificity, which had already been reported to have an anti-metastatic activity. The inhibitory effect of anti-β-galactosyl antibody was completely canceled by adding lactose (galactose-β-1, 4-glucose) to the medium, indicating that this antibody recognized some antigens with β-galactosyl epitope. Hepatoma cells pretreated with this antibody for 48 h showed reduced invasive activity, while the pretreatment of mesothelial cells with the antibody did not affect hepatoma cells invasion. Anti-β-galactosyl antibody also suppressed hepatoma cells adhesion to mesothelial cells monolayer. These results suggest that natural antibody with anti-β-galactosyl specificity may recognize the β-galactosyl epitope in some adhesion-related molecules on hepatoma cells, thus suppressing adhesion and invasion to mesothelial cells monolayer. These results suggest possible therapeutic uses of this antibody in the treatment of metastatic tumors.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo, 183-8509, Japan, eiyouym@cc.tuat.ac.jp.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Miura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Fujita
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Sakai
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tachikawa
authors.forename: Kazumi
authors.initials: K
authors.lastname: Yagasaki
authors.forename: Daisaburo
authors.initials: D
authors.lastname: Fujimoto