title: Modeling in vitro cholesterol reduction in relation to growth of probiotic Lactobacillus casei.
abstract: Lactobacillus casei LA-1 isolated from a nondairy fermented source was evaluated for its in vitro ability to reduce cholesterol. The bacterium tested positive for bile salt deconjugation in relation to cholesterol removal. Tested growth-associated physiological variables such as pH, temperature and inoculum size were all found to have significant effects on in vitro cholesterol reduction and biomass production (both P < 0.005). Furthermore, a central composite design was used to evaluate the effects of significant variables and their interactions. A linear regression model was developed for in vitro cholesterol reduction as a function of growth-associated variables. Maximum cholesterol reduction achieved was 45% whereas maximum biomass yield of 2.34 optical density was observed at the central point. Our study possibly indicates that the growth of L. casei LA-1 depends on its cholesterol removing ability.
authors.affiliation: Department of Biotechnology and Environmental Sciences, Thapar University, Patiala, 147004, India.
authors.forename: Alok
authors.initials: A
authors.lastname: Kumar
authors.forename: Mukesh
authors.initials: M
authors.lastname: Kumar
authors.forename: Moushumi
authors.initials: M
authors.lastname: Ghosh
authors.forename: Abhijit
authors.initials: A
authors.lastname: Ganguli
mesh_terms: D001709:Biotechnology / Q000379:methods; D001711:Biotransformation; D002784:Cholesterol / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007780:Lacticaseibacillus casei / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*; D015233:Models, Statistical; D008969:Molecular Sequence Data; D019936:Probiotics; D017422:Sequence Analysis, DNA; D013696:Temperature
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D002784:Cholesterol