title: Immunofluorescence with dual microwave retrieval of paraffin-embedded sections in the assessment of human renal biopsy specimens.
abstract: Immunofluorescence of frozen tissue sections (IF-F) is a classic technique for renal immunopathologic examination. However, it has certain disadvantages, such as diffuse antigen distribution and few or even no glomeruli in the section. We developed a new technique of immunofluorescence staining using dual microwave retrieval in paraffin-embedded renal tissue sections (IF-DMP) and compared IF-DMP with IF-F in 406 renal biopsy samples. IF-DMP detected significantly more glomeruli than did IF-F (P< .001). There was no significant difference for the specificity and sensitivity in the detection of immunoglobulins, complements, κ, and λ between IF-F and IF-DMP. Concordant observations were 98% for all immunofluorescence, complements, κ, and λ staining and 100% for immunoglobulin staining. Both techniques were completely accurate in confirming diagnoses of various glomerular diseases. IF-DMP provided clearer images of tissue structure and more precise localization of antigens, and it is a suitable alternative for traditional IF-F in clinical renal immunopathologic diagnosis.
authors.affiliation: Dept of Nephrology, State Key Lab of Kidney Diseases, Chinese PLA General Hospital, Beijing, China.
authors.forename: Suozhu
authors.initials: S
authors.lastname: Shi
authors.forename: Qingli
authors.initials: Q
authors.lastname: Cheng
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zheng
authors.forename: Xue-Yuan
authors.initials: XY
authors.lastname: Bai
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Chen
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D001706:Biopsy; D005455:Fluorescent Antibody Technique / Q000379:methods*; D005629:Frozen Sections; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D007668:Kidney / Q000378:metabolism / Q000473:pathology*; D007674:Kidney Diseases / Q000378:metabolism / Q000473:pathology*; D007678:Kidney Glomerulus / Q000378:metabolism / Q000473:pathology; D008872:Microwaves; D016612:Paraffin Embedding; D011237:Predictive Value of Tests
chemical_list: D015415:Biomarkers; D007136:Immunoglobulins