title: Survival of Mycobacterium avium subsp. paratuberculosis in synthetic human gastric juice and acidified porcine bile.
abstract: The bactericidal activities of synthetic gastric juice and acidified porcine bile on Mycobacterium avium subsp. paratuberculosis were assessed using propidium monoazide (PMA)-mediated quantitative reverse transcription-PCR, which allowed rapid relative quantitative analysis of viable M. avium subsp. paratuberculosis cells.
authors.affiliation: Department of Microbiology, University College Cork, Cork, Ireland.
authors.forename: J P
authors.initials: JP
authors.lastname: Dalton
authors.forename: C
authors.initials: C
authors.lastname: Hill
mesh_terms: D000143:Acids / Q000633:toxicity; D000818:Animals; D001386:Azides / Q000378:metabolism; D001646:Bile / Q000382:microbiology*; D004791:Enzyme Inhibitors / Q000378:metabolism; D005750:Gastric Juice / Q000382:microbiology*; D006801:Humans; D050296:Microbial Viability; D008962:Models, Theoretical; D016927:Mycobacterium avium subsp. paratuberculosis / Q000187:drug effects / Q000502:physiology*; D011419:Propidium / Q000031:analogs & derivatives / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D013552:Swine
chemical_list: D000143:Acids; D001386:Azides; D004791:Enzyme Inhibitors; C533957:propidium monoazide; D011419:Propidium