title: The efficacy of eprinomectin extended-release injection against naturally acquired nematode parasites of cattle, with special regard to inhibited fourth-stage Ostertagia larvae.
abstract: The efficacy of eprinomectin in an extended-release injection (ERI) formulation in the treatment of cattle harboring naturally acquired nematode populations (including inhibited nematodes) was evaluated. Five studies were conducted under a similar protocol in the USA, the UK, and in Germany. All study animals were infected by grazing naturally contaminated pastures. The adequacy of pasture infectivity was confirmed by examining tracer calves prior to allocation and treatment of the study animals. The cattle were of various breeds or crosses, weighing 79-491 kg, and aged approximately 6-15 months. In each study, 20 animals were infected by grazing, and then removed from pasture and housed in a manner to preclude further nematode infections for 8-16 days until treatment. Animals were blocked based on descending pre-treatment body weight and randomly allocated to one of two treatments: ERI vehicle (control) at 1 mL/50 kg body weight or eprinomectin 5% (w/v) ERI at 1 mL/50 kg body weight (1.0 mg eprinomectin/kg). Treatments were administered once on Day 0 by subcutaneous injection in front of the shoulder. For parasite recovery and count, all study animals were humanely euthanized 14/15 days after treatment. Cattle treated with eprinomectin ERI had significantly (p<0.05) fewer of the following nematodes than the controls with overall reduction of parasite counts of ≥94%: adult Dictyocaulus viviparus, Capillaria spp., Cooperia oncophora, Cooperia pectinata, Cooperia punctata, Cooperia surnabada, Haemonchus placei, Nematodirus helvetianus, Oesophagostomum radiatum, Ostertagia lyrata, Ostertagia ostertagi, Trichostrongylus axei, Trichostrongylus colubriformis, Trichuris discolor, Trichuris skrjabini, and Trichuris spp.; developing fourth-stage larvae of Ostertagia spp. and Trichostrongylus spp.; and inhibited fourth-stage larvae of Cooperia spp., Haemonchus spp., Nematodirus spp., Oesophagostomum spp., Ostertagia spp., and Trichostrongylus spp. Animal treatments were well accepted, with no adverse reactions to treatment observed in any study animals. The results of this series of controlled studies demonstrated high therapeutic efficacy and acceptability of eprinomectin ERI against pulmonary nematodes and a wide range of gastrointestinal parasitic infections, including inhibited gastrointestinal nematodes, in cattle.
authors.affiliation: Merial Limited, Duluth, GA 30096-4640, USA. james.hunter@merial.com
authors.forename: J S
authors.initials: JS
authors.lastname: Hunter
authors.forename: S
authors.initials: S
authors.lastname: Yoon
authors.forename: T A
authors.initials: TA
authors.lastname: Yazwinski
authors.forename: J C
authors.initials: JC
authors.lastname: Williams
authors.forename: S
authors.initials: S
authors.lastname: Rehbein
mesh_terms: D000818:Animals; D000969:Antinematodal Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy* / Q000469:parasitology; D005260:Female; D007267:Injections; D007559:Ivermectin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D007814:Larva; D008297:Male; D009349:Nematode Infections / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary*; D010028:Ostertagia / Q000502:physiology*; D011897:Random Allocation
chemical_list: D000969:Antinematodal Agents; D007559:Ivermectin; C101434:eprinomectin