title: De novo characterization of Lentinula edodes C(91-3) transcriptome by deep Solexa sequencing.
abstract: Lentinula edodes, has been utilized as food, as well as, in popular medicine, moreover, its extract isolated from its mycelium and fruiting body have shown several therapeutic properties. Yet little is understood about its genes involved in these properties, and the absence of L.edodes genomes has been a barrier to the development of functional genomics research. However, high throughput sequencing technologies are now being widely applied to non-model species. To facilitate research on L.edodes, we leveraged Solexa sequencing technology in de novo assembly of L.edodes C(91-3) transcriptome. In a single run, we produced more than 57 million sequencing reads. These reads were assembled into 28,923 unigene sequences (mean size=689bp) including 18,120 unigenes with coding sequence (CDS). Based on similarity search with known proteins, assembled unigene sequences were annotated with gene descriptions, gene ontology (GO) and clusters of orthologous group (COG) terms. Our data provides the first comprehensive sequence resource available for functional genomics studies in L.edodes, and demonstrates the utility of Illumina/Solexa sequencing for de novo transcriptome characterization and gene discovery in a non-model mushroom.
authors.affiliation: Department of Medical Microbiology, Dalian Medical University, 9 Western Section, Lvshun South Road, Lvshunkou District, Dalian 116044, China.
authors.forename: Mintao
authors.initials: M
authors.lastname: Zhong
authors.forename: Ben
authors.initials: B
authors.lastname: Liu
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Yongzhi
authors.initials: Y
authors.lastname: Lun
authors.forename: Xingyun
authors.initials: X
authors.lastname: Li
authors.forename: Anhong
authors.initials: A
authors.lastname: Ning
authors.forename: Jing
authors.initials: J
authors.lastname: Cao
authors.forename: Min
authors.initials: M
authors.lastname: Huang
mesh_terms: D016681:Genome, Fungal / Q000235:genetics*; D023281:Genomics; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D020942:Shiitake Mushrooms / Q000235:genetics*; D059467:Transcriptome