title: A quantitative analysis to unveil specific binding proteins for bioactive compounds.
abstract: The mechanisms of the action of bioactive compounds discovered via 'black box' assays using phenotypic indicators generally remain unknown, with the major challenges being the identification of target proteins. In this study, we aimed to develop an efficient methodology to unveil target proteins that are rarely characterised. Proof-of-concept experiments were performed using N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), a well-known calmodulin (CaM) antagonist, as a bait compound. The results showed that in our methodology CaM was identified as a W-7-specific binding protein in the cytosolic fraction of a rat brain extract, whereas other proteins acquired in the same experiment were recognised as non-specific binding proteins. The binding affinity of CaM to W-7 is not very high (dissociation constant: 1.6 × 10(-5) M), showing that the recognition specificity is applicable to compounds with very low binding affinities.
authors.affiliation: Discovery Screening Center, Mitsubishi Tanabe Pharma, 2-2-50 Kawagishi, Toda-shi, Saitama 335-8505, Japan. arai.tomonori@me.mt-pharma.co.jp
authors.forename: Tomonori
authors.initials: T
authors.lastname: Arai
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Uehata
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Akatsuka
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Kamiyama
mesh_terms: D000818:Animals; D001665:Binding Sites; D001923:Brain Chemistry; D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D002147:Calmodulin / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D013449:Sulfonamides / Q000494:pharmacology; D014020:Tissue Extracts / Q000737:chemistry
chemical_list: D002147:Calmodulin; D002352:Carrier Proteins; D013449:Sulfonamides; D014020:Tissue Extracts; C017967:W 7; D002118:Calcium