title: Lipoxygenase products in the urine correlate with renal function and body temperature but not with acute transplant rejection.
abstract: Acute transplant rejection is the leading cause of graft loss in the first months after kidney transplantation. Lipoxygenase products mediate pro- and anti-inflammatory actions and thus we aimed to correlate the histological reports of renal transplant biopsies with urinary lipoxygenase products concentrations to evaluate their role as a diagnostic marker. This study included a total of 34 kidney transplant recipients: 17 with an acute transplant rejection and 17 controls. LTE4, LTB4, 12-HETE and 15-HETE concentrations were measured by enzyme immunoassay. Urinary lipoxygenase product concentrations were not significantly changed during an acute allograft rejection. Nevertheless, LTB4 concentrations correlated significantly with the body temperature (P ≤ 0.05) 3 months after transplantation, and 12- and 15-HETE concentrations correlated significantly with renal function (P ≤ 0.05) 2 weeks after transplantation. In conclusion, our data show a correlation for LTB4 with the body temperature 3 months after transplantation and urinary 12- and 15-HETE concentrations correlate positively with elevated serum creatinine concentrations but do not predict acute allograft rejection.
authors.affiliation: Klinik und Poliklinik für Innere Medizin II-Nephrologie, Universität Regensburg, Franz-Josef-Strauß-Allee 11, 93053 Regensburg, Germany. stephan.reinhold@klinik.uni-regensburg.de
authors.forename: Stephan W
authors.initials: SW
authors.lastname: Reinhold
authors.forename: Thomas
authors.initials: T
authors.lastname: Scherl
authors.forename: Benjamin
authors.initials: B
authors.lastname: Stölcker
authors.forename: Tobias
authors.initials: T
authors.lastname: Bergler
authors.forename: Ute
authors.initials: U
authors.lastname: Hoffmann
authors.forename: Christian
authors.initials: C
authors.lastname: Weingart
authors.forename: Miriam C
authors.initials: MC
authors.lastname: Banas
authors.forename: Dmitrij
authors.initials: D
authors.lastname: Kollins
authors.forename: Martin C
authors.initials: MC
authors.lastname: Kammerl
authors.forename: Bernd
authors.initials: B
authors.lastname: Krüger
authors.forename: Bernhard
authors.initials: B
authors.lastname: Kaess
authors.forename: Bernhard K
authors.initials: BK
authors.lastname: Krämer
authors.forename: Bernhard
authors.initials: B
authors.lastname: Banas
mesh_terms: D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid / Q000652:urine*; D000208:Acute Disease; D000328:Adult; D001831:Body Temperature; D005260:Female; D006084:Graft Rejection / Q000201:enzymology / Q000209:etiology / Q000473:pathology / Q000652:urine*; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000652:urine*; D007668:Kidney / Q000502:physiology*; D016030:Kidney Transplantation / Q000009:adverse effects; D007975:Leukotriene B4 / Q000652:urine*; D017999:Leukotriene E4 / Q000652:urine*; D008084:Lipoxygenase / Q000378:metabolism; D008297:Male; D008875:Middle Aged
chemical_list: D006893:Hydroxyeicosatetraenoic Acids; D007975:Leukotriene B4; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D017999:Leukotriene E4; D008084:Lipoxygenase