title: [Effects of Qufengtongluo Recipe on expressions of cell cycle regulatory proteins in rat mesangial cells].
abstract: OBJECTIVE
  To observe the effects of Qufengtongluo Recipe (QFTLR) on the expressions of cell cycle regulatory proteins in rat mesangial cells (MCs) in vitro and investigate the mechanism by which QFTLR inhibits MC proliferation.
  
  
  METHODS
  Using the methods of serum pharmacology, we studied the expressions of cell cycle regulatory proteins in rat MCs treated with QFTLR by laser scanning confocal microscope and immunohistochemistry.
  
  
  RESULTS
  Compared with the normal control cells, the cells challenged with lipopolysaccharide (LPS) showed significantly enhanced expressions of cyclin D1, CDK2, and P21 (P<0.01) and obviously lowered protein expression of P27 (P<0.01). Treatment of the LPS-challenged cells with QFTLR and benazepril both resulted in significantly attenuated expressions of cyclin D1, CDK2, and P21 and obvious increase of P27 expression (P<0.05 or P<0.01), but QFTLR produced stronger effects than benazepril in regulating of cyclinD1, P21 and P27 protein expressions (P<0.05 or P<0.01).
  
  
  CONCLUSIONS
  QFTLR inhibits rat MC proliferation in vitro possibly by down-regulating the cellular expressions of cyclin D1, CDK2, and P21 and up-regulating the expression of P27 protein.
authors.affiliation: Department of Integrated Traditional Chinese and Western Medicine, Second Affiliated Hospital, Xi'an Jiaotong University College of Medicine, Xi'an 710004, China. wuxili1984@163.com
authors.forename: Xili
authors.initials: X
authors.lastname: Wu
authors.forename: Wansen
authors.initials: W
authors.lastname: Sun
authors.forename: Bingyu
authors.initials: B
authors.lastname: Ye
authors.forename: Peng
authors.initials: P
authors.lastname: An
authors.forename: Xingmin
authors.initials: X
authors.lastname: Shi
authors.forename: Rongguo
authors.initials: R
authors.lastname: Fu
authors.forename: Zhu
authors.initials: Z
authors.lastname: Wang
authors.forename: Chenglin
authors.initials: C
authors.lastname: Qiao
mesh_terms: D000818:Animals; D002460:Cell Line; D019938:Cyclin D1 / Q000378:metabolism; D051357:Cyclin-Dependent Kinase 2 / Q000378:metabolism; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D050527:Mesangial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C530106:Ccnd1 protein, rat; C493958:Cdkn1a protein, rat; C493962:Cdkn1b protein, rat; D050759:Cyclin-Dependent Kinase Inhibitor p21; D004365:Drugs, Chinese Herbal; D019938:Cyclin D1; D050760:Cyclin-Dependent Kinase Inhibitor p27; C495892:Cdk2 protein, rat; D051357:Cyclin-Dependent Kinase 2