title: A case of corneal endotheliitis with mumps virus RNA in aqueous humor detected by rt-PCR.
abstract: OBJECTIVE
  To report a case of corneal endotheliitis following the mumps parotitis.
  
  
  METHODS
  A 46-year-old man noticed ciliary infection and visual loss in the right eye after mumps parotitis. To determine the cause of the disease, reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify mumps virus RNA in samples from the aqueous humor.
  
  
  RESULTS
  RT-PCR revealed mumps virus RNA in an aqueous humor sample. Although full recovery of vision was achieved within 1 month of the onset, corneal endothelial cells were severely damaged.
  
  
  CONCLUSIONS
  The authors have detected mumps virus in the aqueous humor by RT-PCR for the first time.
authors.affiliation: Department of Ophthalmology, Yokohama City University School of Medicine, Yokohama, Japan.
authors.forename: Kiyomu
authors.initials: K
authors.lastname: Ando
authors.forename: Mami
authors.initials: M
authors.lastname: Ishihara
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Kusumoto
authors.forename: Etsuko
authors.initials: E
authors.lastname: Shibuya
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nakamura
authors.forename: Nobuhisa
authors.initials: N
authors.lastname: Mizuki
mesh_terms: D001082:Aqueous Humor / Q000821:virology; D003937:Diagnosis, Differential; D004728:Endothelium, Corneal / Q000473:pathology / Q000821:virology*; D015828:Eye Infections, Viral / Q000175:diagnosis / Q000821:virology*; D006801:Humans; D007634:Keratitis / Q000175:diagnosis / Q000821:virology*; D008297:Male; D008875:Middle Aged; D009107:Mumps / Q000175:diagnosis / Q000821:virology*; D009109:Mumps virus / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D012367:RNA, Viral