title: Interleukin-4, interleukin-5, and interleukin-13 gene expression in cultured mononuclear cells from porcine circovirus type 2-vaccinated pigs after cells were challenged with porcine circovirus type 2 open reading frame 2 antigen.
abstract: OBJECTIVE
  To characterize the kinetics of interleukin (IL)-4, IL-5, and IL-13 secretion in peripheral blood and lymph node mononuclear cells isolated from porcine circovirus type 2 (PCV2)-vaccinated pigs after cells were challenged with PCV2 open reading frame 2 antigen.
  
  
  METHODS
  10 pigs.
  
  
  METHODS
  5 pigs were vaccinated with a PCV2 vaccine and received a booster dose 3 weeks later. They were kept together with a similar group of 5 nonvaccinated pigs that served as controls. One week after the second vaccination, peripheral blood mononuclear cells (PBMCs) and excised retropharyngeal lymph node mononuclear cells (LNMCs) were isolated and cultured. Cells were then challenged by exposure to PCV2 open reading frame 2 and evaluated at 2, 12, 24, and 48 hours to determine the expression of IL-4, IL-5, and IL-13 via quantitative PCR assay. Changes in gene expression were analyzed relative to the results from analysis of the sample at 0 hours (calibrator).
  
  
  RESULTS
  All ILs were upregulated differently in LNMCs and PBMCs from vaccinated pigs. Lymph node mononuclear cells from vaccinated animals produced significantly more IL-4 mRNA than did PBMCs at 2, 12, and 48 hours (relative change: 2.8 vs -3.6, 13.0 vs 3.6, and 9.8 vs 1.8, respectively) and more IL-5 mRNA at 2, 12, 24, and 48 hours (relative change: 1. 2 vs -4.8, 2.2 vs 0.2, 3.2 vs -1.9, and 4.0 vs -3.6, respectively). Interleukin-13 mRNA reached its highest concentration at 24 hours but was 11.9-fold higher in PBMCs than in LNMCs.
  
  
  CONCLUSIONS
  Results supported the importance of IL-4, IL-5, and IL-13 in pigs, suggesting that PBMCs and LNMCs express cytokines in a tissue-specific manner.
authors.affiliation: Department of Animal Production, Faculty of Veterinary Medicine, University of Murcia, Murcia, Spain. jjquere@um.es
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Quereda
authors.forename: Guillermo
authors.initials: G
authors.lastname: Ramis
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Pallarés
authors.forename: Ludivine
authors.initials: L
authors.lastname: Chapat
authors.forename: Anne
authors.initials: A
authors.lastname: Goubier
authors.forename: François
authors.initials: F
authors.lastname: Joisel
authors.forename: Catherine
authors.initials: C
authors.lastname: Charreyre
authors.forename: David
authors.initials: D
authors.lastname: Villar
authors.forename: Antonio
authors.initials: A
authors.lastname: Muõoz
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D002478:Cells, Cultured; D017925:Circovirus / Q000276:immunology*; D018076:DNA, Complementary / Q000378:metabolism; D005786:Gene Expression Regulation; D018793:Interleukin-13 / Q000235:genetics* / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics* / Q000378:metabolism; D015848:Interleukin-5 / Q000235:genetics* / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D016366:Open Reading Frames / Q000276:immunology; D053570:Porcine Postweaning Multisystemic Wasting Syndrome / Q000276:immunology* / Q000821:virology; D012313:RNA / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000821:virology
chemical_list: D000956:Antigens, Viral; D018076:DNA, Complementary; D018793:Interleukin-13; D015848:Interleukin-5; D015847:Interleukin-4; D012313:RNA