title: Structural model of lymphocyte receptor NKR-P1C revealed by mass spectrometry and molecular modeling.
abstract: NKR-P1C is an activating immune receptor expressed on the surface of mouse natural killer cells. It has been widely used as a marker for NK cell identification in different mice strains. Recently we solved a crystal structure of the C-type lectin-like domain of a homologous protein, NKR-P1A, using X-ray crystallography and also described the strategy for rapid characterization of the protein conformation in solution. This procedure utilized chemical cross-linking, hydrogen/deuterium exchange, and molecular modeling. It was found that the solution structure differs from the crystal structure in the conformation of the loop region. The loop, detached from the protein compact core in the crystal structure, is closely attached to the core of the protein in solution. Here we present and interpret the solution structure of the C-type lectin-like domain of NKR-P1C using chemical cross-linking and molecular modeling. The validation of the model and conformation of the loop region in NKR-P1C were addressed using ion-mobility mass spectrometry.
authors.affiliation: Department of Biochemistry, Charles University, Prague, Czech Republic.
authors.forename: Daniel
authors.initials: D
authors.lastname: Rozbesky
authors.forename: Zofie
authors.initials: Z
authors.lastname: Sovova
authors.forename: Julien
authors.initials: J
authors.lastname: Marcoux
authors.forename: Petr
authors.initials: P
authors.lastname: Man
authors.forename: Rudiger
authors.initials: R
authors.lastname: Ettrich
authors.forename: Carol V
authors.initials: CV
authors.lastname: Robinson
authors.forename: Petr
authors.initials: P
authors.lastname: Novak
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000950:Antigens, Ly / Q000737:chemistry* / Q000378:metabolism*; D018360:Crystallography, X-Ray / Q000379:methods; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D008958:Models, Molecular; D008969:Molecular Sequence Data; D055651:NK Cell Lectin-Like Receptor Subfamily B / Q000737:chemistry* / Q000378:metabolism*; D017433:Protein Structure, Secondary
chemical_list: D000950:Antigens, Ly; C527211:Klrb1c protein, mouse; D055651:NK Cell Lectin-Like Receptor Subfamily B