title: A monoclonal antibody specific to glucosyltransferase B of Streptococcus mutans GS-5 and its glucosyltransferase inhibitory efficiency.
abstract: Glucosyltransferase-B (GTFB) of Streptococcus mutans is considered a virulence factor because of its activity in the production of insoluble glucan, which is key to the bacterial attachment onto dental surfaces, leading to the formation of dental caries. Local passive immunization with monoclonal antibodies against GTFB is considered to be an effective way to prevent dental caries. Here we amplified a 1.3 kb fragment of the N-terminal half of the gtfB gene (193-1530) of S. mutans by PCR and expressed the truncated protein (GTFBN). The expressed, purified protein was used as an immunogen in BALB/c mice. We selected and established one hybridoma (HBN8) that was capable of producing anti-GTFBN using ELISA, dot blot, and Western blot analyses. The monoclonal anti-GTFBN antibody was purified by affinity chromatography and its isotype was confirmed as IgG2a. The anti-GTFBN antibody inhibited the enzymatic activity of crude glucosyltransferase of S. mutans GS-5 in a dose-dependent manner. These data suggest that the anti-GTFBN antibody could be used as a vaccine to prevent the aggregation of S. mutans on tooth surfaces, and thus prevent the formation of dental caries.
authors.affiliation: Department of Pediatric Dentistry and Institute of Oral Bioscience, School of Dentistry, Jeonju-si, Jeollabuk-do, Republic of Korea.
authors.forename: Mi-Ah
authors.initials: MA
authors.lastname: Kim
authors.forename: Min-Jeong
authors.initials: MJ
authors.lastname: Lee
authors.forename: Hae-Kyoung
authors.initials: HK
authors.lastname: Jeong
authors.forename: Hee-Jeong
authors.initials: HJ
authors.lastname: Song
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Jeon
authors.forename: Kyung-Yeol
authors.initials: KY
authors.lastname: Lee
authors.forename: Jae-Gon
authors.initials: JG
authors.lastname: Kim
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry* / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D005260:Female; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D006825:Hybridomas; D007074:Immunoglobulin G / Q000737:chemistry* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D013295:Streptococcus mutans / Q000201:enzymology
chemical_list: D000907:Antibodies, Bacterial; D058846:Antibodies, Monoclonal, Murine-Derived; D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D007074:Immunoglobulin G; C036655:1,3-alpha-D-glucan synthase; D005964:Glucosyltransferases