title: Filling the gaps - the generation of full genomic sequences for 15 common and well-documented HLA class I alleles using next-generation sequencing technology.
abstract: Many common and well-documented (CWD) HLA alleles have only been partially characterized. The DNA sequence of these incomplete alleles, as published in the IMGT/HLA database, is most often limited to exons that code for the extracellular domains of the mature protein. Here we describe the application of next-generation sequencing technology to obtain full length genomic sequence from a single long-range PCR amplicon for 15 common and well-documented HLA Class I alleles. This technology is well suited to fill in the gaps of the current HLA allele sequence database which is largely incomplete. A more comprehensive catalog of HLA allele sequences would be beneficial in the evaluation of mismatches in transplantation, studies of population genetics, the evolution of HLAs, regulatory mechanisms and HLA expression, and issues related to the genomic organization of the MHC.
authors.affiliation: Dept. of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, PA 19014, USA.
authors.forename: C
authors.initials: C
authors.lastname: Lind
authors.forename: D
authors.initials: D
authors.lastname: Ferriola
authors.forename: K
authors.initials: K
authors.lastname: Mackiewicz
authors.forename: A
authors.initials: A
authors.lastname: Papazoglou
authors.forename: A
authors.initials: A
authors.lastname: Sasson
authors.forename: D
authors.initials: D
authors.lastname: Monos
mesh_terms: D000483:Alleles; D015698:Genomic Library; D006680:HLA Antigens / Q000235:genetics*; D015234:HLA-A Antigens / Q000235:genetics; D015235:HLA-B Antigens / Q000235:genetics; D015236:HLA-C Antigens / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results
chemical_list: D006680:HLA Antigens; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015236:HLA-C Antigens