title: CK2 enzyme affinity against c-myc 424-434 substrate in human lung cancer tissue.
abstract: CK2 is a serine threonine kinase that participates in a variety of cellular processes with more than 300 defined substrates. This critical enzyme is known to be upregulated in cancers, but the role of this upregulation in carcinogenesis is not yet fully understood but c-myc, one of the defined CK2 substrates, is a well-known proto- oncogene that is normally essential in developmental process but is also involved in tumor development. We evaluated the optimal enzyme and substrate concentrations for CK2 activity in both neoplastic and non-neoplastic human lung tissues using the c-myc 424-434 peptide (EQKLISEEDL) as a substrate. The activities measured for the neoplastic tissue were 600-750 U/mg protein while those for the control tissue was in the range of 650-800 U/ mg. Km value for c-myc peptide was determined as 0.33 μM in non-neoplastic tissue and 0.18 μM in neoplastic tissue. In this study, we did not observe an increased activity in the neoplastic tissue when compared with the non-neoplastic lung tissue, but we recorded two times higher affinity for c-myc 424-434 in cancer tissue. Considering the metabolic position of c-myc 424-434, our results suggest that phosphorylation by CK2 may be important in dimerization and thus it might affect the regulation of c-myc in cancer tissues.
authors.affiliation: Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey. ilhanyaylim@gmail.com
authors.forename: Ilhan
authors.initials: I
authors.lastname: Yaylim
authors.forename: Nazli Ezgi
authors.initials: NE
authors.lastname: Ozkan
authors.forename: Turgut
authors.initials: T
authors.lastname: Isitmangil
authors.forename: Gulbu
authors.initials: G
authors.lastname: Isitmangil
authors.forename: Akif
authors.initials: A
authors.lastname: Turna
authors.forename: Turgay
authors.initials: T
authors.lastname: Isbir
mesh_terms: D000595:Amino Acid Sequence; D047390:Casein Kinase II / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008168:Lung / Q000378:metabolism*; D008175:Lung Neoplasms / Q000378:metabolism*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism*; D011379:Prognosis; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism*
chemical_list: C000715470:MAS1 protein, human; D010446:Peptide Fragments; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc; D047390:Casein Kinase II