title: In vitro and in vivo anti-inflammatory effects of 4-methoxy-5- hydroxycanthin-6-one, a natural alkaloid from Picrasma quassioides.
abstract: In the present study, we evaluated the anti-inflammatory effect of 4-methoxy-5- hydroxycanthin-6-one (CAN), a natural alkaloid isolated from Picrasma quassioides. CAN significantly inhibited the production of NO and the release of TNF-α induced by LPS in macrophage RAW 264.7. Western blot showed that CAN can downregulate the expression of iNOS protein. After oral administration, CAN (3, 9, and 27 mg/kg) reduced the development of carrageenan-induced paw edema and complete Freund's adjuvant (CFA)-induced chronic arthritis in rats. The observed results indicated that pre-treatment with CAN might be an effective therapeutic intervention against inflammatory diseases including chronic arthritis.
authors.affiliation: School of Pharmacy, Yantai University, 264005 Yantai, Shandong, PR China.
authors.forename: Huaying
authors.initials: H
authors.lastname: Fan
authors.forename: Dong
authors.initials: D
authors.lastname: Qi
authors.forename: Mingyan
authors.initials: M
authors.lastname: Yang
authors.forename: Hui
authors.initials: H
authors.lastname: Fang
authors.forename: Ke
authors.initials: K
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D001168:Arthritis / Q000139:chemically induced / Q000188:drug therapy*; D002351:Carrageenan; D002460:Cell Line; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D007211:Indoles / Q000494:pharmacology / Q000627:therapeutic use*; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009287:Naphthyridines / Q000494:pharmacology / Q000627:therapeutic use*; D009569:Nitric Oxide / Q000378:metabolism; D008517:Phytotherapy; D032270:Picrasma / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014992:Xylenes
chemical_list: D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D007211:Indoles; D008070:Lipopolysaccharides; D009287:Naphthyridines; D014409:Tumor Necrosis Factor-alpha; D014992:Xylenes; D009569:Nitric Oxide; D002351:Carrageenan; C539437:nigakinone