title: Functioning of metal contaminated garden soil after remediation.
abstract: The effect of remediation using three EDTA doses (10, 30, 60 mmol kg(-1)) on soil functioning was assessed using column experiment and Brassica rapa. Soil washing removed up to 77, 29 and 72% of metals from soil contaminated with 1378, 578 and 8.5 mg kg(-1) of Pb, Zn and Cd, respectively. Sequential extraction indicated removal from the carbonate soil fraction. Metal oral-accessibility from the stomach phase was reduced by up to 75 and from the small intestine by up to 79% (Pb). Part of metals (up to 0.8% Cd) was lost due to leaching from columns. Remediation reduced toxic metal soil-root transfer by up to 61% but did not prevent metal accumulation in leaves. The fitness of plants grown on EDTA washed soils (gas exchange, fluorescence) was not compromised. Remediation initially reduced the soil DNA content (up to 29%, 30 mmol kg(-1) EDTA) and changed the structure of microbial population.
authors.affiliation: Agronomy Department, Centre for Soil and Environmental Science, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
authors.forename: Masa
authors.initials: M
authors.lastname: Jelusic
authors.forename: Helena
authors.initials: H
authors.lastname: Grcman
authors.forename: Dominik
authors.initials: D
authors.lastname: Vodnik
authors.forename: Metka
authors.initials: M
authors.lastname: Suhadolc
authors.forename: Domen
authors.initials: D
authors.lastname: Lestan
mesh_terms: D004492:Edetic Acid / Q000737:chemistry; D052918:Environmental Restoration and Remediation / Q000379:methods*; D051639:Gardening; D008670:Metals / Q000032:analysis / Q000737:chemistry*; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000737:chemistry*
chemical_list: D008670:Metals; D012987:Soil; D012989:Soil Pollutants; D004492:Edetic Acid