title: Hyperthermia inhibits homologous recombination repair and sensitizes cells to ionizing radiation in a time- and temperature-dependent manner.
abstract: Hyperthermia has long been known as a radio-sensitizing agent that displays anti-tumor effects, and has been developed as a therapeutic application. The mechanisms of hyperthermia-induced radio-sensitization are highly associated with inhibition of DNA repair. Our investigations aimed to show how hyperthermia inactivate homologous recombination repair in the process of sensitizing cells to ionizing radiation by using a series of DNA repair deficient Chinese Hamster cells. Significant differences in cellular toxicity attributable to hyperthermia at and above 42.5°C were observed. In wild-type and non-homologous end joining repair mutants, cells in late S phase showed double the amount heat-induced radio-sensitization effects of G1-phase cells. Both radiation-induced DNA double strand breaks and chromatin damage resulting from hyperthermia exposure was measured to be approximately two times higher in G2-phase cells than G0/G1 cells. Additionally, G2-phase cells took approximately two times as long to repair DNA damage over time than G0/G1-phase cells. To supplement these findings, radiation-induced Rad51 foci formations at DNA double strand break sites were observed to gradually dissociate in response to the temperature and time of hyperthermia exposure. Dissociated Rad51 proteins subsequently re-formed foci at damage sites with time, and occurred in a trend also related to temperature and time of hyperthermia exposure. These findings suggest Rad51's dissociation and subsequent reformation at DNA double strand break sites in response to varying hyperthermia conditions plays an important role in hyperthermia-induced radio-sensitization.
authors.affiliation: Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, Colorado 80523, USA.
authors.forename: Stefan C
authors.initials: SC
authors.lastname: Genet
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Fujii
authors.forename: Junko
authors.initials: J
authors.lastname: Maeda
authors.forename: Masami
authors.initials: M
authors.lastname: Kaneko
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Genet
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Miyagawa
authors.forename: Takamitsu A
authors.initials: TA
authors.lastname: Kato
mesh_terms: D000818:Animals; D016466:CHO Cells; D002453:Cell Cycle; D002460:Cell Line; D002470:Cell Survival; D006224:Cricetinae; D003412:Cricetulus; D053903:DNA Breaks, Double-Stranded; D006657:Histones / Q000378:metabolism; D006801:Humans; D006979:Hyperthermia, Induced; D009154:Mutation; D051135:Rad51 Recombinase / Q000378:metabolism; D011836:Radiation Tolerance; D059767:Recombinational DNA Repair / Q000235:genetics; D013696:Temperature; D013997:Time Factors
chemical_list: C468783:H2AX protein, human; D006657:Histones; C495153:RAD51 protein, human; D051135:Rad51 Recombinase