title: High expression of STIM1 in the early stages of diffuse axonal injury.
abstract: Increased intracellular calcium ([Ca(2+)](i)) is a key pathological mechanism involved in secondary neuronal injury and cell death due to diffuse axonal injury (DAI). To date, this increased [Ca(2+)](i) is believed to be mainly caused by dysfunction of voltage-gated sodium channels and mechanoporation of the plasma membrane. Store-operated calcium entry (SOCE) is another source of Ca(2+) influx, and stromal interaction molecule 1 (STIM1) is considered as a sensor and a regulator of SOCE. In this study, we established a DAI in vivo model in rats by lateral head rotation. Using immunohistochemistry, real-time RT-PCR and Western blot, we investigated STIM1 expression levels in the cerebral cortex of rats after lateral head rotational injury. Results revealed notably high STIM1 expression in neurons in the early stages (within 24 h) of DAI. STIM1 began to increase at 6 h post-injury (PI) peaked at 12 h PI, and then gradually decreased. At 2 days PI, STIM1 expression in the injury group showed no significant difference compared with that of the control group. These results indicate that abnormal SOCE may participate in Ca(2+) overload of neurons in the early stages after DAI via enhanced STIM1 expression.
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital of the Medical College of Xi'an Jiaotong University, Xi'an 710061, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Jinning
authors.initials: J
authors.lastname: Song
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.forename: Jiyang
authors.initials: J
authors.lastname: An
authors.forename: Peng
authors.initials: P
authors.lastname: Sun
authors.forename: Dandong
authors.initials: D
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Jin
authors.forename: Junfeng
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D020833:Diffuse Axonal Injury / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000032:analysis / Q000096:biosynthesis*; D046529:Microscopy, Electron, Transmission; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000071737:Stromal Interaction Molecule 1
chemical_list: D008562:Membrane Glycoproteins; C518857:Stim1 protein, rat; D000071737:Stromal Interaction Molecule 1