title: RelAp43, a member of the NF-κB family involved in innate immune response against Lyssavirus infection.
abstract: NF-κB transcription factors are crucial for many cellular processes. NF-κB is activated by viral infections to induce expression of antiviral cytokines. Here, we identified a novel member of the human NF-κB family, denoted RelAp43, the nucleotide sequence of which contains several exons as well as an intron of the RelA gene. RelAp43 is expressed in all cell lines and tissues tested and exhibits all the properties of a NF-κB protein. Although its sequence does not include a transactivation domain, identifying it as a class I member of the NF-κB family, it is able to potentiate RelA-mediated transactivation and stabilize dimers comprising p50. Furthermore, RelAp43 stimulates the expression of HIAP1, IRF1, and IFN-β - three genes involved in cell immunity against viral infection. It is also targeted by the matrix protein of lyssaviruses, the agents of rabies, resulting in an inhibition of the NF-κB pathway. Taken together, our data provide the description of a novel functional member of the NF-κB family, which plays a key role in the induction of anti-viral innate immune response.
authors.affiliation: Institut Pasteur, Unité Dynamique des Lyssavirus et Adaptation à l'Hôte, Paris, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Luco
authors.forename: Olivier
authors.initials: O
authors.lastname: Delmas
authors.forename: Pierre-Olivier
authors.initials: PO
authors.lastname: Vidalain
authors.forename: Frédéric
authors.initials: F
authors.lastname: Tangy
authors.forename: Robert
authors.initials: R
authors.lastname: Weil
authors.forename: Hervé
authors.initials: H
authors.lastname: Bourhy
mesh_terms: D005786:Gene Expression Regulation / Q000235:genetics / Q000276:immunology*; D006367:HeLa Cells; D006801:Humans; D007113:Immunity, Innate; D050856:Interferon Regulatory Factor-1 / Q000235:genetics / Q000276:immunology; D016899:Interferon-beta / Q000235:genetics / Q000276:immunology; D018114:Lyssavirus / Q000276:immunology*; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000276:immunology; D018353:Rhabdoviridae Infections / Q000235:genetics / Q000276:immunology*; D051996:Transcription Factor RelA / Q000235:genetics / Q000276:immunology*
chemical_list: C108259:HiAT1 transporter; C494257:IRF1 protein, human; D050856:Interferon Regulatory Factor-1; D009004:Monosaccharide Transport Proteins; C498143:RELA protein, human; D051996:Transcription Factor RelA; D016899:Interferon-beta