title: Protective effect of Cordyceps polysaccharide on hydrogen peroxide-induced             mitochondrial dysfunction in HL-7702 cells.
abstract: Multiple reports have suggested that reactive oxygen species (ROS) are implicated             in hepatic fibrosis and that they are capable of causing hepatocyte apoptosis             in hepatic fibrosis by causing oxidative damage to the liver. Thus, the study             of antioxidant compounds may shed light on the treatment of hepatic fibrosis.             The aim of the current study was to investigate the protective effects of Cordyceps             polysaccharide (CPS), a major antioxidative component of Cordyceps militaris,             on hydrogen peroxide (H2O2)-induced cell apoptosis. The data showed that CPS markedly             inhibited H2O2-induced mitochondrial dysfunction, lowered cell viability, increased             the apoptotic rate, boosted ROS production, decreased mitochondrial membrane potential             (MMP), reduced the intracellular adenosine triphosphate (ATP) level, increased             the Bax/Bcl-2 ratio and promoted cytochrome C (Cyt C) release. These results indicated             that CPS protected HL-7702 cells, which are used as the main model of hepatic             fibrosis, against H2O2-induced mitochondrial dysfunction by decreasing ROS production             and regulating mitochondrial apoptotic signaling through the Cyt C, Bax and Bcl-2             apoptosis-related proteins.
authors.affiliation: Department of Cellular and Genetic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, PR China.
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Liu
authors.forename: Qiukai
authors.initials: Q
authors.lastname: E
authors.forename: Ji
authors.initials: J
authors.lastname: Zuo
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Tao
authors.forename: Wen
authors.initials: W
authors.lastname: Liu
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D032661:Cordyceps / Q000378:metabolism*; D045304:Cytochromes c / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D011134:Polysaccharides / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000975:Antioxidants; D011134:Polysaccharides; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D000255:Adenosine Triphosphate; D045304:Cytochromes c; D006861:Hydrogen Peroxide