title: Immune responses and protective efficacy of a recombinant swinepox virus co-expressing HA1 genes of H3N2 and H1N1 swine influenza virus in mice and pigs.
abstract: The recombinant swine poxvirus rSPV/H3-2A-H1 co-expressing HA1 genes of H3N2 and H1N1 subtype SIV has been constructed and identified. Inoculations of rSPV/H3-2A-H1 yielded ELISA and neutralization antibodies against SIV H1N1 and H3N2, and elicited potent H1N1 and H3N2 SIV-specific INF-γ response from T-lymphocytes in mice and pigs in this study. Complete protection against SIV H1N1 or H3N2 challenge in pigs was observed.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou 730046, China. xu1jiarong@gmail.com
authors.forename: Jiarong
authors.initials: J
authors.lastname: Xu
authors.forename: Dongyan
authors.initials: D
authors.lastname: Huang
authors.forename: Jiaping
authors.initials: J
authors.lastname: Xu
authors.forename: Shichao
authors.initials: S
authors.lastname: Liu
authors.forename: Huixing
authors.initials: H
authors.lastname: Lin
authors.forename: Haodan
authors.initials: H
authors.lastname: Zhu
authors.forename: Bao
authors.initials: B
authors.lastname: Liu
authors.forename: Wen
authors.initials: W
authors.lastname: Chen
authors.forename: Chengping
authors.initials: C
authors.lastname: Lu
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics* / Q000276:immunology; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics* / Q000276:immunology; D053122:Influenza A Virus, H3N2 Subtype / Q000235:genetics* / Q000276:immunology; D007252:Influenza Vaccines / Q000235:genetics* / Q000276:immunology* / Q000494:pharmacology; D007371:Interferon-gamma / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D011897:Random Allocation; D018148:Suipoxvirus / Q000235:genetics* / Q000276:immunology; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D013601:T-Lymphocytes / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology / Q000494:pharmacology
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D014614:Vaccines, Synthetic; D007371:Interferon-gamma