title: Differential dynamic engagement within 24 SH3 domain: peptide complexes revealed by co-linear chemical shift perturbation analysis.
abstract: There is increasing evidence for the functional importance of multiple dynamically populated states within single proteins. However, peptide binding by protein-protein interaction domains, such as the SH3 domain, has generally been considered to involve the full engagement of peptide to the binding surface with minimal dynamics and simple methods to determine dynamics at the binding surface for multiple related complexes have not been described. We have used NMR spectroscopy combined with isothermal titration calorimetry to comprehensively examine the extent of engagement to the yeast Abp1p SH3 domain for 24 different peptides. Over one quarter of the domain residues display co-linear chemical shift perturbation (CCSP) behavior, in which the position of a given chemical shift in a complex is co-linear with the same chemical shift in the other complexes, providing evidence that each complex exists as a unique dynamic rapidly inter-converting ensemble. The extent the specificity determining sub-surface of AbpSH3 is engaged as judged by CCSP analysis correlates with structural and thermodynamic measurements as well as with functional data, revealing the basis for significant structural and functional diversity amongst the related complexes. Thus, CCSP analysis can distinguish peptide complexes that may appear identical in terms of general structure and percent peptide occupancy but have significant local binding differences across the interface, affecting their ability to transmit conformational change across the domain and resulting in functional differences.
authors.affiliation: Program in Molecular Structure and Function, Hospital for Sick Children, Toronto, Ontario, Canada. elliott.stollar@enmu.edu
authors.forename: Elliott J
authors.initials: EJ
authors.lastname: Stollar
authors.forename: Hong
authors.initials: H
authors.lastname: Lin
authors.forename: Alan R
authors.initials: AR
authors.lastname: Davidson
authors.forename: Julie D
authors.initials: JD
authors.lastname: Forman-Kay
mesh_terms: D002151:Calorimetry / Q000379:methods*; D016000:Cluster Analysis; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000737:chemistry*; D018909:src Homology Domains
chemical_list: D010455:Peptides