title: Dietary intake of melatonin from tropical fruit altered urinary excretion of 6-sulfatoxymelatonin in healthy volunteers.
abstract: This study assessed the melatonin content of six tropical fruits and examined whether human consumption could contribute to dietary melatonin as measured by 6-sulfatoxymelatonin (aMT6-s, a marker of circulating melatonin in the body). Melatonin was extracted using methanol and analyzed by high-performance liquid chromatography. In a clinical crossover study, 30 healthy volunteers consumed selected fruits one at a time, with a 1week wash-out period between fruits, until completing all six fruits. Most fruits had moderate melatonin content. Significant increases in urine aMT6-s concentrations were seen after the consumption of pineapple (266%, p = 0.004), banana (180%, p = 0.001), and orange (47%, p = 0.007). The need to analyze melatonin both in fruit and as in vivo uptake was demonstrated. Further study is warranted regarding the clinical effect of fruit consumption in people with age-related melatonin reduction problems such as sleeplessness and illnesses involving oxidative damage.
authors.affiliation: Faculty of Pharmaceutical Sciences and Melatonin Research Group, Khon Kaen University, Khon Kaen 40002, Thailand. pnutja@kku.ac.th
authors.forename: Nutjaree Pratheepawanit
authors.initials: NP
authors.lastname: Johns
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Johns
authors.forename: Supatra
authors.initials: S
authors.lastname: Porasuphatana
authors.forename: Preeyaporn
authors.initials: P
authors.lastname: Plaimee
authors.forename: Manit
authors.initials: M
authors.lastname: Sae-Teaw
mesh_terms: D000293:Adolescent; D000328:Adult; D031230:Ananas / Q000737:chemistry; D032084:Citrus sinensis / Q000737:chemistry; D018592:Cross-Over Studies; D004032:Diet; D005260:Female; D005638:Fruit / Q000737:chemistry*; D006293:Health Promotion; D006801:Humans; D008297:Male; D008550:Melatonin / Q000008:administration & dosage* / Q000031:analogs & derivatives* / Q000652:urine; D028521:Musa / Q000737:chemistry; D014329:Tropical Climate; D055815:Young Adult
chemical_list: C054513:6-sulfatoxymelatonin; D008550:Melatonin