title: The miR-183/96/182 cluster regulates oxidative apoptosis and sensitizes cells to chemotherapy in gliomas.
abstract: Many microRNAs reside in clusters in the genome, are generally similar in sequence, are transcribed in the same direction, and usually function synergistically. The miR-183/96/182 cluster is composed of 3 miRNA genes, and increased expression of miR-183, 96 and 182 are implicated in glioma carcinogenesis. Knockdown of individual components or of the entire miR-183/96/182 cluster inhibits the survival of glioma cells by regulating the ROS-induced apoptosis pathway. Furthermore, inhibition of the miR-183/96/182 cluster induced ROS-mediated AKT/survival independent of three target genes FGF9, CPEB1, and FOXO1, and inhibition of the miRNA cluster induced p53/apoptosis signaling, which was dependent on these same genes. In addition, knockdown of the miR-183/96/182 cluster enhanced the anticancer effect of Temozolomide on glioma cells by the ROS-mediated apoptosis pathway. Therefore, the miR-183/96/182 cluster may be a pleiotropic target for glioma therapy.
authors.affiliation: Cancer Research Institute, Central South University, Changsha 410008, Hunan, P.R. China.
authors.forename: Hailin
authors.initials: H
authors.lastname: Tang
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Bian
authors.forename: Chaofeng
authors.initials: C
authors.lastname: Tu
authors.forename: Zeyou
authors.initials: Z
authors.lastname: Wang
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Yu
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: Gang
authors.initials: G
authors.lastname: Xu
authors.forename: Minghua
authors.initials: M
authors.lastname: Wu
authors.forename: Guiyuan
authors.initials: G
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D001483:Base Sequence; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D017931:DNA Primers; D005910:Glioma / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D018384:Oxidative Stress; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; C533449:MIRN183 microRNA, human; C541492:MIRN96 microRNA, human; D035683:MicroRNAs; C538984:Mirn182 microRNA, human