title: [Inhibitory effect of cyclopamine on metastatic ability of EC109 cells and its mechanism].
abstract: OBJECTIVE
  To investigate the effect of cyclopamine on metastatic ability of human esophageal cancer EC109 cells and explore the possible mechanism.
  
  
  METHODS
  Transwell chamber assay and angiogenesis assay were used to examine the metastatic ability, invasiveness and angiogenesis of EC109 cells treated with cyclopamine for 48 h. The expression of Gli-1 mRNA was detected using RT-PCR, and Western blotting was used to examine the protein expressions of Gli-1, matrix metalloproteinase-9 (MMP-9) and vascular endothelial growth factor (VEGF).
  
  
  RESULTS
  Inhibition of the hedgehog signaling pathway by cyclopamine suppressed the migration, invasion, and angiogenesis of EC109 cells. Cyclopamine treatment significantly lowered the expression of Gli-1 mRNA (P<0.05) and the protein expressions of Gli-1, MMP-9 and VEGF (P<0.05).
  
  
  CONCLUSIONS
  Cyclopamine can significantly inhibit the metastatic capacity of EC109 cells possibly by down-regulating MMP-9 and VEGF expression as a result of Gli-1 inhibition.
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China. 492588955@qq.com
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Zuo
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Qin
authors.forename: Kaibin
authors.initials: K
authors.lastname: Wang
authors.forename: Xiangru
authors.initials: X
authors.lastname: Zheng
authors.forename: Liqian
authors.initials: L
authors.lastname: Chen
mesh_terms: D045744:Cell Line, Tumor; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009362:Neoplasm Metastasis; D012333:RNA, Messenger / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects; D014157:Transcription Factors / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014704:Veratrum Alkaloids / Q000494:pharmacology*; D000071676:Zinc Finger Protein GLI1
chemical_list: C400551:GLI1 protein, human; D012333:RNA, Messenger; D014157:Transcription Factors; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D014704:Veratrum Alkaloids; D000071676:Zinc Finger Protein GLI1; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9; C000541:cyclopamine