title: Real-Time PCR assays for quantification of qnr genes in environmental water samples and chicken feces.
abstract: Real-time PCR assays were developed for the enumeration of plasmid-mediated quinolone resistance (PMQR) determinants, such as the qnrA, qnrB, and qnrS genes, in different water samples and chicken feces. The results indicate that the developed assays are specific and sensitive for the quantification of qnr genes in complex samples.
authors.affiliation: University of Girona, Girona, Spain. emarti@icra.cat
authors.forename: Elisabet
authors.initials: E
authors.lastname: Marti
authors.forename: José Luis
authors.initials: JL
authors.lastname: Balcázar
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002645:Chickens; D024881:Drug Resistance, Bacterial; D005243:Feces / Q000382:microbiology; D010957:Plasmids / Q000032:analysis*; D015363:Quinolones / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014871:Water Microbiology
chemical_list: D000900:Anti-Bacterial Agents; D015363:Quinolones