title: Does preincubation with prostaglandin E1 or prostaglandin E2 enhance oxytocin-induced myometrial contractility in vitro?
abstract: OBJECTIVE
  To determine if preincubation with prostaglandin E1 (PGE1) and E2 (PGE2) enhances oxytocin-induced myometrial contractility in vitro.
  
  
  METHODS
  Myometrial strips from 13 women were incubated with PGE1 (10-5 mol/L or 10-6 mol/L), PGE2 (10-5 mol/L or 10-6 mol/L) or solvent before adding cumulative concentrations of oxytocin (10-10 to 10-6 mol/L). The area under the contraction curve was calculated after addition of each agent. One- and two-way analysis of variance was used for comparison (significance p < 0.05).
  
  
  RESULTS
  PGE2 10-5 mol/L reduced response to oxytocin 10-9 to 10-6 mol/L (p < 0.05). PGE2 reduced spontaneous myometrial contractility as compared with PGE1 (p < 0.05). A dose-dependent negative effect of prostaglandins was detected on oxytocin 10-8 mol/L (10-5 mol/L > 10-6 mol/L; p < 0.05).
  
  
  CONCLUSIONS
  Contrary to the hypothesis, neither PGE1 nor PGE2 enhanced oxytocin-induced myometrial contractility; in fact, PGE2 decreased contractility.
authors.affiliation: Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, TX 77555-0144, USA. ossidi74seppie@yahoo.it
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Chiossi
authors.forename: Luckey C
authors.initials: LC
authors.lastname: Reed
authors.forename: Maged M
authors.initials: MM
authors.lastname: Costantine
authors.forename: Gary D V
authors.initials: GD
authors.lastname: Hankins
authors.forename: George R
authors.initials: GR
authors.lastname: Saade
authors.forename: Monica
authors.initials: M
authors.lastname: Longo
mesh_terms: D000328:Adult; D000527:Alprostadil / Q000008:administration & dosage*; D000704:Analysis of Variance; D019540:Area Under Curve; D015232:Dinoprostone / Q000008:administration & dosage*; D004357:Drug Synergism; D005260:Female; D006801:Humans; D009215:Myometrium / Q000187:drug effects*; D010121:Oxytocin / Q000494:pharmacology*; D046509:Tissue Culture Techniques; D014590:Uterine Contraction / Q000187:drug effects* / Q000502:physiology
chemical_list: D010121:Oxytocin; D000527:Alprostadil; D015232:Dinoprostone