title: Novosphingobium aquaticum sp. nov., isolated from the humic-matter-rich bog lake Grosse Fuchskuhle.
abstract: A yellow-pigmented, Gram-negative rod, designated FNE08-86(T), was isolated from subsurface water of the humic-matter-rich and almost-neutral north-east basin of the experimentally divided bog lake Grosse Fuchskuhle (Brandenburg, Germany). Analysis of the nearly full-length 16S rRNA gene sequence showed the highest 16S rRNA gene sequence similarity with Novosphingobium rosa IAM 14222(T) (96.3 %). Sequence similarities with all other members of the genus Novosphingobium species were <96 %, but phylogenetic tree construction clearly showed the placement of strain FNE08-86(T) within the genus Novosphingobium. The predominant fatty acids were C18 : 1ω7c and C16 : 0, and only a single 2-hydroxy fatty acid, C14 : 0 2-OH, was detected. The polar lipid profile revealed phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine as major compounds, with smaller amounts of sphingoglycolipid, phosphatidylmonomethylethanolamine, diphosphatidylglycerol and several unidentified lipids. In the quinone system ubiquinone Q-10 was predominant and in the polyamine pattern spermidine was predominant. Characterization by genotypic, chemotaxonomic and phenotypic analysis indicated that strain FNE08-86(T) represents a novel species of the genus Novosphingobium, for which we propose the name Novosphingobium aquaticum sp. nov. (type strain FNE08-86(T) = DSM 25088(T) = CCM 7983(T)).
authors.affiliation: Institut für Mikrobiologie und Molekularbiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.|Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors.forename: Stefanie P
authors.initials: SP
authors.lastname: Glaeser
authors.affiliation: Laboratorium für Zellbiologie, Philipps-Universität Marburg, D-35043 Marburg, Germany.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Bolte
authors.affiliation: Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität Wien, A-1210 Wien, Austria.
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Busse
authors.affiliation: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Kämpfer
authors.affiliation: Leibniz Institute of Freshwater Ecology and Inland Fisheries, Stechlin, Germany.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Grossart
authors.affiliation: Institut für Mikrobiologie und Molekularbiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Glaeser
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005858:Germany; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000032:analysis; D014871:Water Microbiology; D053833:Wetlands
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C024989:coenzyme Q10