title: [Sequence polymorphism and mapping of wheat Ca2+-binding protein TaCRT-A gene].
abstract: Taking thirty-seven hexaploid wheat (AABBDD) accessions with different drought resistance at seedling stage, three wheat species with A genome (AA), and three tetraploid wheat species (AABB) as test materials, and by direct sequencing the single nucleotide polymorphism (SNP) in TaCRT-A, this paper analyzed the relationships of the SNP with the drought resistance of wheat ( Triticum aestivum) at its seedling stage, and mapped the TaCRT-A on the chromosome of wheat. The full-length sequence of the TaCRT-A genomic DNA was 3887 bp. A total of 202 nucleotide variant loci were observed in the full length sequence of 167141 bp, among which, 165 SNP and 37 InDel with the frequencies of 1 SNP/1013 bp and 1 InDel/4517 bp were detected, respectively. The nucleotide diversity (pi) in coding region of TaCRT-A was lower than that in non-coding region, suggesting that the selection pressure in coding region was stronger than that in non-coding region. The 43 accessions could be classified as 14 haplotypes (H1-H14) by haploid analysis, among which, H1, H2, and H13 all contained one accession which was the donor species of A genome in common wheat, H16 and H7 had one high drought-resistant accession, H8 comprised tetraploid wheat, drought-resistant accessions, and drought-sensitive accessions, whereas H11 included the wheat accessions with drought-resistance and medium-drought resistance. Though the expression of TaCRT was induced by water stress, no significant relationship was identified between TaCRT-A polymorphism and drought resistance. Using a population of recombinant inbred lines derived from a cross of Opata 85 x W7984, the TaCRT-A was mapped between SSR markers Xmwg30 and Xmwg570 on chromosome 3A, and the genetic distances were 10.5 cM and 49.6 cM from the flanking markers, respectively.
authors.affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement/Ministry of Agriculture Key Laboratory of Crop Germplasm and Utilization, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China. sxndwjp@163.com
authors.forename: Ji-Ping
authors.initials: JP
authors.lastname: Wang
authors.forename: Xin-Guo
authors.initials: XG
authors.lastname: Mao
authors.forename: Run-Zhi
authors.initials: RZ
authors.lastname: Li
authors.forename: Rui-Lian
authors.initials: RL
authors.lastname: Jing
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D002135:Calcium-Binding Proteins / Q000235:genetics*; D037282:Calreticulin / Q000235:genetics*; D055864:Droughts; D017343:Genes, Plant; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D014908:Triticum / Q000235:genetics*
chemical_list: D002135:Calcium-Binding Proteins; D037282:Calreticulin