title: Gene transfection efficacy assessment of human cervical cancer cells using dual-mode fluorescence microendoscopy.
abstract: We report a novel approach to quantitatively assess gene transfection efficacy using dual-modality microendoscopy that can simultaneously monitor both laser scanning reflectance and fluorescence imaging. The system uses a 500-μm-diameter coherent fiber bundle and permits 3.5-μm lateral resolution. Both reflectance and fluorescence images obtained from two silicon avalanche photodetectors are displaying at 1 Hz and processed automatically to calculate gene transfection efficiency (the ratio of fluorescent cells among the total cells). To validate the system performance we examined the expression of cyan fluorescent protein using human cervical cancer cells (HeLa) in four commercially available reagents. The result was compared with that using a high-resolution bench-top microscope.
authors.affiliation: Department of Electrical and Computer Engineering, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218,  USA.
authors.forename: Jaepyeong
authors.initials: J
authors.lastname: Cha
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Saumya
authors.initials: S
authors.lastname: Gurbani
authors.forename: Gyeong Woo
authors.initials: GW
authors.lastname: Cheon
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Jin U
authors.initials: JU
authors.lastname: Kang
keywords: (060.2350) Fiber optics imaging; (170.2150) Endoscopic imaging; (170.2520) Fluorescence microscopy