title: New cyclomyrsinol diterpenes from Euphorbia aellenii with their immunomodulatory effects.
abstract: Chloroform-acetone extract of the aerial parts of Euphorbia aellenii Rech. f. (Euphorbiaceae) was investigated for its diterpenoidal constituents. This led to the isolation of two new and one known cyclomyrsinol-type diterpenes 1-3. The structures were elucidated on the basis of 1D and 2D (1)H and (13)C NMR techniques, and in vitro immunomodulatory activity was evaluated by standard proliferation of human peripheral blood lymphocytes. Results showed that all the three compounds were found to inhibit lymphocyte proliferation significantly (p < 0.05) at 50 μg/ml concentration. Among them, compound 2 showed more activity against phytohemagglutinin-activated T-cell proliferation with an IC(50) of 40.4 ± 9.35 μg/ml.
authors.affiliation: Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Islamic Republic of Iran. ghannadian@gmail.com
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ghanadian
authors.forename: M Iqbal
authors.initials: MI
authors.lastname: Choudhary
authors.forename: Abdul Majid
authors.initials: AM
authors.lastname: Ayatollahi
authors.forename: M Ahmad
authors.initials: MA
authors.lastname: Mesaik
authors.forename: Omer Mohamed
authors.initials: OM
authors.lastname: Abdalla
authors.forename: Suleiman
authors.initials: S
authors.lastname: Afsharypour
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D004224:Diterpenes / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D028481:Euphorbia / Q000737:chemistry*; D006801:Humans; D007155:Immunologic Factors / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D007492:Iran; D008214:Lymphocytes / Q000187:drug effects; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: C579446:3,5,10,14,15-pentaacetyl-8-isobutanoyl-cyclomyrsinol; C579445:3-nicotinyl-5,10,14,15-tetraacetyl-8-(2',3'-dimethylbutanoyl)cyclomyrsinol; D004224:Diterpenes; D007155:Immunologic Factors