title: Proteome and peptidome of human acquired enamel pellicle on deciduous teeth.
abstract: Understanding the composition and structure of the acquired enamel pellicle (AEP) has been a major goal in oral biology. Our lab has conducted studies on the composition of AEP formed on permanent enamel. The exhaustive exploration has provided a comprehensive identification of more than 100 proteins from AEP formed on permanent enamel. The AEP formed on deciduous enamel has not been subjected to the same biochemical characterization scrutiny as that of permanent enamel, despite the fact that deciduous enamel is structurally different from permanent enamel. We hypothesized that the AEP proteome and peptidome formed on deciduous enamel may also be composed of unique proteins, some of which may not be common with AEP of permanent enamel explored previously. Pellicle material was collected from 10 children (aged 18-54 months) and subjected to mass spectrometry analysis. A total of 76 pellicle proteins were identified from the deciduous pellicle proteome. In addition, 38 natural occurring AEP peptides were identified from 10 proteins, suggesting that primary AEP proteome/peptidome presents a unique proteome composition. This is the first study to provide a comprehensive investigation of in vivo AEP formed on deciduous enamel.
authors.affiliation: Schulich School of Medicine & Dentistry, The University Western University, London, ON N5A6C1, Canada. walter.siqueira@uwo.ca.
authors.forename: Jason N
authors.initials: JN
authors.lastname: Zimmerman
authors.forename: William
authors.initials: W
authors.lastname: Custodio
authors.forename: Sahza
authors.initials: S
authors.lastname: Hatibovic-Kofman
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Lee
authors.forename: Yizhi
authors.initials: Y
authors.lastname: Xiao
authors.forename: Walter L
authors.initials: WL
authors.lastname: Siqueira
mesh_terms: D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D044622:Dental Pellicle / Q000378:metabolism*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D010455:Peptides / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D014094:Tooth, Deciduous / Q000378:metabolism*
chemical_list: D010455:Peptides; D020543:Proteome