title: The biodisposition and hypertrichotic effects of bimatoprost in mouse skin.
abstract: Studies on bimatoprost were performed with two objectives: (i) to determine whether bimatoprost possesses hair growth-stimulating properties beyond eyelash hypertrichosis and (ii) to investigate the biodisposition of bimatoprost in skin for the first time. Bimatoprost, at the dose used clinically for eyelash growth (0.03%) and given once daily for 14 days, increased pelage hair growth in C57/black 6 mice. This occurred as a much earlier onset of new hair growth in shaved mice and the time taken to achieve complete hair regrowth, according to photographic documentation and visual assessment. Bimatoprost biodisposition in the skin was determined at three concentrations: 0.01%, 0.03% and 0.06%. Dose-dependent C(max) values were obtained (3.41, 6.74, 12.3 μg/g tissue), and cutaneous bimatoprost was well maintained for 24 h following a single dose. Bimatoprost was recovered from the skin only as the intact molecule, with no detectable levels of metabolites. Thus, bimatoprost produces hypertrichosis as the intact molecule.
authors.affiliation: Department of Biological Sciences, Allergan, Inc, Irvine, CA 92612, USA.
authors.forename: David F
authors.initials: DF
authors.lastname: Woodward
authors.forename: Elaine S-H
authors.initials: ES
authors.lastname: Tang
authors.forename: Mayssa
authors.initials: M
authors.lastname: Attar
authors.forename: Jenny W
authors.initials: JW
authors.lastname: Wang
mesh_terms: D000577:Amides / Q000493:pharmacokinetics* / Q000494:pharmacology*; D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology; D000069580:Bimatoprost; D003008:Cloprostenol / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005140:Eyelashes; D006197:Hair / Q000502:physiology; D006983:Hypertrichosis / Q000139:chemically induced* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D011453:Prostaglandins / Q000378:metabolism; D012867:Skin / Q000187:drug effects* / Q000473:pathology; D013997:Time Factors
chemical_list: D000577:Amides; D000959:Antihypertensive Agents; D011453:Prostaglandins; D003008:Cloprostenol; D000069580:Bimatoprost