title: Purification and biochemical characterization of a highly thermostable bacteriocin isolated from Brevibacillus brevis strain GM100.
abstract: A bacteriocin-producing (11,000 AU mL(-1)) strain was isolated from the rhizosphere of healthy Algerian plants Ononis angustissima Lam., and identified as Brevibacillus brevis strain GM100. The bacteriocin, called Bac-GM100, was purified to homogeneity from the culture supernatant, and, based on MALDI-TOF/MS analysis, was a monomer protein with a molecular mass of 4375.66 Da. The 21 N-terminal residues of Bac-GM100 displayed 65% homology with thurincin H from Bacillus thuringiensis. Bac-GM100 was extremely heat-stable (20 min at 120 °C), and was stable within a pH range of 3-10. It proved sensitive to various proteases, which demonstrated its protein nature. It was also found to display a bactericidal mode of action against gram-negative (Salmonella enteric ATCC 43972, Pseudomonas aeruginosa ATCC 49189, and Agrobacterium tumefaciens C58) and gram-positive (Enterococcus faecalis ENSAIA 631 and Staphylococcus aureus ATCC 6538) bacteria, and a fungistatic mode of action against the pathogenic fungus Candida tropicalis R2 CIP 203.
authors.affiliation: Laboratory of Applied Microbiology, Department of Microbiology, Faculty of Natural and Life Sciences, University Ferhat Abbas, Setif, Algeria. mouloud_ghadbane@yahoo.fr
authors.forename: Mouloud
authors.initials: M
authors.lastname: Ghadbane
authors.forename: Daoud
authors.initials: D
authors.lastname: Harzallah
authors.forename: Atef Ibn
authors.initials: AI
authors.lastname: Laribi
authors.forename: Bassem
authors.initials: B
authors.lastname: Jaouadi
authors.forename: Hani
authors.initials: H
authors.lastname: Belhadj
mesh_terms: D000462:Algeria; D000890:Anti-Infective Agents / Q000302:isolation & purification* / Q000378:metabolism* / Q000494:pharmacology; D001413:Bacillus thuringiensis / Q000737:chemistry; D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000378:metabolism* / Q000494:pharmacology; D001430:Bacteriocins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism* / Q000494:pharmacology; D058675:Brevibacillus / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D041022:Candida tropicalis / Q000187:drug effects / Q000254:growth & development; D013293:Enterococcus faecalis / Q000187:drug effects / Q000254:growth & development; D007887:Fabaceae / Q000382:microbiology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D050296:Microbial Viability / Q000187:drug effects; D008970:Molecular Weight; D010802:Phylogeny; D055550:Protein Stability; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D058441:Rhizosphere; D019779:Salmonella enterica / Q000187:drug effects / Q000254:growth & development; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development
chemical_list: D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D001430:Bacteriocins; C568940:thurincin H