title: Detection of Epstein-Barr virus and genotyping based on EBNA2 protein in Mexican patients with hodgkin lymphoma: a comparative study in children and adults.
abstract: BACKGROUND
  Epstein-Barr virus (EBV) is a member of the Herpesviridae family and is associated with Hodgkin lymphoma (HL). Isolates of EBV are classified according to sequence variation in the latency genes such as Epstein-Barr virus nuclear antigen (EBNA). EBNA2 contains the most divergent locus and is classified into type 1 and type 2 or EBNA2A and EBNA2B, respectively. We compared the frequency of EBV and the distribution of EBNA genotypes in Mexican children and adults with HL.
  
  
  METHODS
  Lymph node biopsy specimens from children and adults with HL were embedded in paraffin. EBV was identified by LMP1 amplification and Epstein-Barr-encoded RNA EBER by in situ hybridization (ISH) and genotyped as EBNA2A or EBNA2B using nested polymerase chain reaction (PCR) and specific primers for the detection of subtype.
  
  
  RESULTS
  Sixty-six samples were obtained from 3 hospitals-42 (63%) from children and 24 (37%) from adults with HL. Thirty-two of the 42 samples (76.1%) were positive for EBV in children and 16 of 24 (66.6%) samples were positive in adults (P = .41). In both children and adults, EBV was found more frequently in male patients. Thirty-four of 48 cases could be typed (70.8%). EBNA2A was found in 7/21 (33.3%) children and in 4/13 (30.8%) adults (P = 1.0), and EBNA2B was found in 10/21 (47.6%) children and in 9/13 (69.2%) adults (P = .22). A mix of subtypes was found in 4/21 (19%) children.
  
  
  CONCLUSIONS
  EBV was found frequently in both children and adults with HL. EBNA2B was the most frequent subtype, and a high frequency of mixed subtypes was found in children.
authors.affiliation: Laboratorio de Morfologia Celular, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón, CP. 11340 DF, México.
authors.forename: Icela
authors.initials: I
authors.lastname: Palma
authors.forename: Ana Elena
authors.initials: AE
authors.lastname: Sánchez
authors.forename: Elva
authors.initials: E
authors.lastname: Jiménez-Hernández
authors.forename: Francisco
authors.initials: F
authors.lastname: Alvarez-Rodríguez
authors.forename: Margarita
authors.initials: M
authors.lastname: Nava-Frias
authors.forename: Pedro
authors.initials: P
authors.lastname: Valencia-Mayoral
authors.forename: Citlatepet
authors.initials: C
authors.lastname: Salinas-Lara
authors.forename: Norma
authors.initials: N
authors.lastname: Velazquez-Guadarrama
authors.forename: Josefina
authors.initials: J
authors.lastname: Portilla-Aguilar
authors.forename: Rocio Y
authors.initials: RY
authors.lastname: Pena
authors.forename: Patricia
authors.initials: P
authors.lastname: Ramos-Salazar
authors.forename: Alejandra
authors.initials: A
authors.lastname: Contreras
authors.forename: Ana
authors.initials: A
authors.lastname: Alfaro
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Espinosa
authors.forename: Nayelli
authors.initials: N
authors.lastname: Nájera
authors.forename: Gisela
authors.initials: G
authors.lastname: Gutierrez
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Mejia-Arangure
authors.forename: José
authors.initials: J
authors.lastname: Arellano-Galindo
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D020031:Epstein-Barr Virus Infections / Q000473:pathology / Q000821:virology*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000235:genetics*; D005260:Female; D005838:Genotype; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006689:Hodgkin Disease / Q000473:pathology / Q000821:virology*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D012189:Retrospective Studies; D014764:Viral Proteins / Q000235:genetics*; D055815:Young Adult
chemical_list: C402641:EBNA-2 protein, Human herpesvirus 4; D019309:Epstein-Barr Virus Nuclear Antigens; D014764:Viral Proteins