title: Engineering B cells with mRNA.
abstract: Ex vivo activated B cells are an alternative source of antigen presenting cells (APC). However, the ability of ex vivo activated B cells to function as potent APCs has been a concern especially when compared to dendritic cells (DC). Herein, we introduce a strategy to modulate antigen presentation and immune stimulation functions of activated B cells by co-transfection with multiple mRNAs encoding costimulatory molecules (OX40L, 4-1BBL, and CD80), cytokines (IL-12p35 and IL-12p40) and antigen. These activated B cells modified to express immune stimulatory molecules can be a potent alternative to DCs in immunotherapy.
authors.affiliation: Department of Surgery, Duke University Medical Center, Durham, NC, USA.
authors.forename: Jaewoo
authors.initials: J
authors.lastname: Lee
authors.forename: David
authors.initials: D
authors.lastname: Boczkowski
authors.forename: Smita
authors.initials: S
authors.lastname: Nair
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D000941:Antigens / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D060846:Cell Engineering / Q000379:methods*; D006801:Humans; D053765:Interleukin-12 Subunit p35 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D053762:Interleukin-12 Subunit p40 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology; D012333:RNA, Messenger / Q000737:chemistry*; D014162:Transfection / Q000379:methods*
chemical_list: D000941:Antigens; D015703:Antigens, CD; C508858:IL12A protein, human; C500879:IL12B protein, human; D053765:Interleukin-12 Subunit p35; D053762:Interleukin-12 Subunit p40; D012333:RNA, Messenger