title: Alteromonas australica sp. nov., isolated from the Tasman Sea.
abstract: A non-pigmented, motile, Gram-negative bacterium designated H 17(T) was isolated from a seawater sample collected in Port Phillip Bay (the Tasman Sea, Pacific Ocean). The new organism displayed optimal growth between 4 and 37 °C, was found to be neutrophilic and slightly halophilic, tolerating salt water environments up to 10 % NaCl. Strain H 17(T) was found to be able to degrade starch and Tween 80 but unable to degrade gelatin or agar. Phosphatidylglycerol (27.7 %) and phosphatidylethanolamine (72.3 %) were found to be the only associated phospholipids. The major fatty acids identified are typical for the genus Alteromonas and include C16:0, C16:1ω7, C17:1ω8 and C18:1ω7. The G+C content of the DNA was found to be 43.4 mol%. A phylogenetic study, based on the 16S rRNA gene sequence analysis and Multilocus Phylogenetic Analysis, clearly indicated that strain H 17(T) belongs to the genus Alteromonas. The DNA-DNA relatedness between strain H 17(T) and the validly named Alteromonas species was between 30.7 and 46.4 mol%. Based on these results, a new species, Alteromonas australica, is proposed. The type strain is H 17(T) (= KMM 6016(T) = CIP 109921(T)).
authors.affiliation: Swinburne University of Technology, PO Box 218, Hawthorn, VIC, 3122, Australia. eivanova@swin.edu.au
authors.forename: Elena P
authors.initials: EP
authors.lastname: Ivanova
authors.forename: Hooi Jun
authors.initials: HJ
authors.lastname: Ng
authors.forename: Hayden K
authors.initials: HK
authors.lastname: Webb
authors.forename: Valeriya V
authors.initials: VV
authors.lastname: Kurilenko
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Zhukova
authors.forename: Valery V
authors.initials: VV
authors.lastname: Mikhailov
authors.forename: Olga N
authors.initials: ON
authors.lastname: Ponamoreva
authors.forename: Russell J
authors.initials: RJ
authors.lastname: Crawford
mesh_terms: D020591:Alteromonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010140:Pacific Ocean; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S