title: Novel human multiple myeloma cell line UHKT-893.
abstract: We established and characterized a new IL-6 dependent multiple myeloma (MM) cell line UHKT-893 from the bone marrow of a relapsed 57-year-old woman.
  
  
  RESULTS
  Using nephelometry, cells with plasma cell phenotype and morphology were found to secrete IgG and free kappa (κ)-light chain of immunoglobulin. κ-Light chain was also recognized intracellularly by flow cytometry and by mass spectrometry. VH4-39 region of IgVH genes was rearranged and somatically hypermutated. Cytogenetic analysis of cells revealed new chromosome abnormalities in all breakpoints unique in both MM patients and cell lines - t(1;6), t(1;11), t(5;15), t(5;21), +der(11;15) and der(16). IL-6 independent subline UHKT-893a was established by adaptation to descending IL-6 concentration, while the original cell line keeps on maintaining its IL-6 dependency.
  
  
  CONCLUSIONS
  The cell line provides a suitable material for cellular and molecular studies of tumor abnormalities, with potentially unique mutagenic features of myeloma disease. It may be utilized for human hybridoma construction and vaccine development. Both IL-6 dependent and independent cell clones represent an important model for studies of myeloma cell growth and resistance emerging during targeted therapy.
authors.affiliation: Department of Cellular Biochemistry, Institute of Hematology and Blood Transfusion (IHBT), Prague, Czech Republic. Lenka.Uherkova@uhkt.cz
authors.forename: Lenka
authors.initials: L
authors.lastname: Uherková
authors.forename: Irena
authors.initials: I
authors.lastname: Vančurová
authors.forename: Ilona
authors.initials: I
authors.lastname: Vyhlídalová
authors.forename: Markéta
authors.initials: M
authors.lastname: Pleschnerová
authors.forename: Ivan
authors.initials: I
authors.lastname: Spička
authors.forename: Romana
authors.initials: R
authors.lastname: Mihalová
authors.forename: Jana
authors.initials: J
authors.lastname: Březinová
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Hodný
authors.forename: Kamila
authors.initials: K
authors.lastname: Cermáková
authors.forename: Veronika
authors.initials: V
authors.lastname: Polanská
authors.forename: Iuri
authors.initials: I
authors.lastname: Marinov
authors.forename: Petr L
authors.initials: PL
authors.lastname: Jedelský
authors.forename: Kateřina
authors.initials: K
authors.lastname: Kuželová
authors.forename: Petr
authors.initials: P
authors.lastname: Stöckbauer
mesh_terms: D001854:Bone Marrow Cells / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D020732:Cytogenetic Analysis; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology; D008875:Middle Aged; D009101:Multiple Myeloma / Q000473:pathology*; D061251:Primary Cell Culture / Q000379:methods; D012008:Recurrence
chemical_list: D015850:Interleukin-6