title: Cell biology of the endoplasmic reticulum and the Golgi apparatus through proteomics.
abstract: Enriched endoplasmic reticulum (ER) and Golgi membranes subjected to mass spectrometry have uncovered over a thousand different proteins assigned to the ER and Golgi apparatus of rat liver. This, in turn, led to the uncovering of several hundred proteins of poorly understood function and, through hierarchical clustering, showed that proteins distributed in patterns suggestive of microdomains in cognate organelles. This has led to new insights with respect to their intracellular localization and function. Another outcome has been the critical testing of the cisternal maturation hypothesis showing overwhelming support for a predominant role of COPI vesicles in the transport of resident proteins of the ER and Golgi apparatus (as opposed to biosynthetic cargo). Here we will discuss new insights gained and also highlight new avenues undertaken to further explore the cell biology of the ER and the Golgi apparatus through tandem mass spectrometry.
authors.affiliation: The Research Institute of the McGill University Health Centre and the Department of Medicine, McGill University, Montreal, Quebec H3A 1A1, Canada.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Smirle
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Au
authors.forename: Michael
authors.initials: M
authors.lastname: Jain
authors.forename: Kurt
authors.initials: K
authors.lastname: Dejgaard
authors.forename: Tommy
authors.initials: T
authors.lastname: Nilsson
authors.forename: John
authors.initials: J
authors.lastname: Bergeron
mesh_terms: D000818:Animals; D037281:Calnexin / Q000378:metabolism; D003585:Cell Biology; D002469:Cell Separation; D016000:Cluster Analysis; D004721:Endoplasmic Reticulum / Q000378:metabolism / Q000502:physiology*; D006056:Golgi Apparatus / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D021381:Protein Transport; D040901:Proteomics / Q000379:methods; D051381:Rats; D053719:Tandem Mass Spectrometry
chemical_list: D037281:Calnexin