title: Structural characteristics and evolution of the Protobothrops elegans pancreatic phospholipase A2 gene in contrast with those of Protobothrops genus venom phospholipase A2 genes.
abstract: The nucleotide sequence of the gene encoding Protobothrops elegans (Crotalinae) pancreatic phospholipase A(2) (PLA(2)), abbreviated PePancPLA(2), was determined by means of inverted PCR techniques. Since its deduced amino acid sequence contains a pancreatic loop and shows high similarity to that of Laticauda semifasciata (Elapinae) group IB pancreatic PLA(2), PePancPLA(2) is classified into group IB PLA(2). The nucleotide sequences of the PePancPLA(2) gene, the L. semifasciata group IB pancreatic PLA(2) gene, and the L. semifasciata group IA venom PLA(2) gene are similar to one another but greatly dissimilar to those of Protobothrops genus (Crotalinae) group II venom PLA(2) genes, suggesting that the Elapinae group IB PLA(2) gene and the group IA PLA(2) gene appeared after Elapinae was established, and that the Crotalinae group II venom PLA(2) genes came into existence before Elapinae and Crotalinae diverged. A phylogenetic analysis of their amino acid sequences confirms this.
authors.affiliation: Department of Applied Life Science, Faculty of Bioscience and Biotechnology, Sojo University, Kumamoto, Japan. chijiwa@life.sojo-u.ac.jp
authors.forename: Takahito
authors.initials: T
authors.lastname: Chijiwa
authors.forename: Hideto
authors.initials: H
authors.lastname: Nakasone
authors.forename: Sakiko
authors.initials: S
authors.lastname: Irie
authors.forename: Naoki
authors.initials: N
authors.lastname: Ikeda
authors.forename: Kantaro
authors.initials: K
authors.lastname: Tomoda
authors.forename: Naoko
authors.initials: N
authors.lastname: Oda-Ueda
authors.forename: Shosaku
authors.initials: S
authors.lastname: Hattori
authors.forename: Motonori
authors.initials: M
authors.lastname: Ohno
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003435:Crotalid Venoms / Q000737:chemistry*; D004546:Elapid Venoms / Q000737:chemistry*; D017815:Elapidae / Q000502:physiology*; D019143:Evolution, Molecular; D007527:Isoenzymes / Q000145:classification / Q000235:genetics; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology; D054467:Phospholipases A2 / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D011994:Recombinant Proteins / Q000145:classification / Q000235:genetics; D016415:Sequence Alignment; D017841:Trimeresurus / Q000502:physiology*
chemical_list: D003435:Crotalid Venoms; D004546:Elapid Venoms; D007527:Isoenzymes; D011994:Recombinant Proteins; D054467:Phospholipases A2