title: Inhibition of Geobacter dechlorinators at elevated trichloroethene concentrations is explained by a reduced activity rather than by an enhanced cell decay.
abstract: Microbial dechlorination of trichloroethene (TCE) is inhibited at elevated TCE concentrations. A batch experiment and modeling analysis were performed to examine whether this self-inhibition is related to an enhanced cell decay or a reduced dechlorination activity at increasing TCE concentrations. The batch experiment combined four different initial TCE concentrations (1.4-3.0 mM) and three different inoculation densities (4.0 × 10(5) to 4.0 × 10(7)Geobacter cells·mL(-1)). Chlorinated ethene concentrations and Geobacter 16S rRNA gene copy numbers were measured. The time required for complete conversion of TCE to cis-DCE increased with increasing initial TCE concentration and decreasing inoculation density. Both an enhanced decay and a reduced activity model fitted the experimental results well, although the reduced activity model better described the lag phase and microbial decay in some treatments. In addition, the reduced activity model succeeded in predicting the reactivation of the dechlorination reaction in treatments in which the inhibiting TCE concentration was lowered after 80 days. In contrast, the enhanced decay model predicted a Geobacter cell density that was too low to allow recovery for these treatments. Conclusively, our results suggest that TCE self-inhibition is related to a reduced dechlorination activity rather than to an enhanced cell decay at elevated TCE concentrations.
authors.affiliation: Division of Soil and Water Management, Department of Earth and Environmental Sciences, KU Leuven (University of Leuven), Kasteelpark Arenberg 20, B-3001 Heverlee, Belgium.
authors.forename: Jo
authors.initials: J
authors.lastname: Philips
authors.forename: Pieter Jan
authors.initials: PJ
authors.lastname: Haest
authors.forename: Dirk
authors.initials: D
authors.lastname: Springael
authors.forename: Erik
authors.initials: E
authors.lastname: Smolders
mesh_terms: D061249:Batch Cell Culture Techniques; D001673:Biodegradation, Environmental / Q000187:drug effects; D004000:Dichloroethylenes / Q000633:toxicity; D018628:Gene Dosage; D045302:Geobacter / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D054879:Halogenation / Q000187:drug effects*; D008954:Models, Biological; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014241:Trichloroethylene / Q000633:toxicity*
chemical_list: D004000:Dichloroethylenes; D012336:RNA, Ribosomal, 16S; D014241:Trichloroethylene; C035384:1,2-dichloroethylene