title: Comparative study of the fatty acid binding process of a new FABP from Cherax quadricarinatus by fluorescence intensity, lifetime and anisotropy.
abstract: Fatty acid-binding proteins (FABPs) are small cytosolic proteins, largely distributed in invertebrates and vertebrates, which accomplish uptake and intracellular transport of hydrophobic ligands such as fatty acids. Although long chain fatty acids play multiple crucial roles in cellular functions (structural, energy metabolism, regulation of gene expression), the precise functions of FABPs, especially those of invertebrate species, remain elusive. Here, we have identified and characterized a novel FABP family member, Cq-FABP, from the hepatopancreas of red claw crayfish Cherax quadricarinatus. We report the characterization of fatty acid-binding affinity of Cq-FABP by four different competitive fluorescence-based assays. In the two first approaches, the fluorescent probe 8-Anilino-1-naphthalenesulfonate (ANS), a binder of internal cavities of protein, was used either by directly monitoring its fluorescence emission or by monitoring the fluorescence resonance energy transfer occurring between the single tryptophan residue of Cq-FABP and ANS. The third and the fourth approaches were based on the measurement of the fluorescence emission intensity of the naturally fluorescent cis-parinaric acid probe or the steady-state fluorescence anisotropy measurements of a fluorescently labeled fatty acid (BODIPY-C16), respectively. The four methodologies displayed consistent equilibrium constants for a given fatty acid but were not equivalent in terms of analysis. Indeed, the two first methods were complicated by the existence of non specific binding modes of ANS while BODIPY-C16 and cis-parinaric acid specifically targeted the fatty acid binding site. We found a relationship between the affinity and the length of the carbon chain, with the highest affinity obtained for the shortest fatty acid, suggesting that steric effects primarily influence the interaction of fatty acids in the binding cavity of Cq-FABP. Moreover, our results show that the binding affinities of several fatty acids closely parallel their prevalences in the hepatopancreas of C. quadricarinatus as measured under specific diet conditions.
authors.affiliation: Laboratoire de Biologie et Pharmacologie Appliquée, CNRS UMR8113, Ecole Normale Supérieure Cachan, Institut d'Alembert, Cachan, France.
authors.forename: Jiayao
authors.initials: J
authors.lastname: Li
authors.forename: Etienne
authors.initials: E
authors.lastname: Henry
authors.forename: Lanmei
authors.initials: L
authors.lastname: Wang
authors.forename: Olivier
authors.initials: O
authors.lastname: Delelis
authors.forename: Huan
authors.initials: H
authors.lastname: Wang
authors.forename: Françoise
authors.initials: F
authors.lastname: Simon
authors.forename: Patrick
authors.initials: P
authors.lastname: Tauc
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Brochon
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Zhao
authors.forename: Eric
authors.initials: E
authors.lastname: Deprez
mesh_terms: D000595:Amino Acid Sequence; D000821:Animal Feed; D000818:Animals; D001483:Base Sequence; D033364:Decapoda; D050556:Fatty Acid-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D005454:Fluorescence Polarization; D008969:Molecular Sequence Data; D011485:Protein Binding
chemical_list: D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids