title: Feasibility of using gelatin-microbial transglutaminase complex to repair experimental retinal detachment in rabbit eyes.
abstract: BACKGROUND
  To investigate the effect of using gelatin-microbial transglutaminase (gelatin-mTG) complex for treating experimental retinal detachment.
  
  
  METHODS
  Vitrectomy with artificial posterior vitreous detachment (PVD) followed by induction of a retinal tear and detachment was performed in rabbit eyes. Gelatin-mTG complex or gelatin alone (control) was placed on the retinal tears. Fundus examination using optical coherence tomography (OCT) was performed after the surgery. Vitrectomy with PVD alone was also performed in additional rabbits. After application of the gelatin-mTG complex on the normal retinal surface, the electroretinogram (ERG) was measured 7 days after surgery.
  
  
  RESULTS
  Gelatin-mTG complex covered the retinal tear for more than 7 days after the vitrectomy, with less prominent inflammation. Reattachment of the retina occurred in all treated eyes. In contrast, massive fibrin materials were observed at 1 day after the surgery in the control group. In addition, OCT showed that all of the gelatin disappeared by day 3. Local retinal detachment remained in three of the eyes. As demonstrated by the ERG, gelatin-mTG complex had no harmful effects on retinal function.
  
  
  CONCLUSIONS
  The results indicate that gelatin-mTG complex continues to adhere and seal retinal tears for at least several days after administration without any inflammatory reaction.
authors.affiliation: Department of Ophthalmology, Saga University Faculty of Medicine, 5-1-1, Nabeshima, Saga 849-8501, Japan.
authors.forename: Soichiro
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Akira
authors.initials: A
authors.lastname: Hirata
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Ishikawa
authors.forename: Keisuke
authors.initials: K
authors.lastname: Ohta
authors.forename: Kei-ichiro
authors.initials: K
authors.lastname: Nakamura
authors.forename: Satoshi
authors.initials: S
authors.lastname: Okinami
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D004596:Electroretinography; D058450:Endotamponade; D005240:Feasibility Studies; D005466:Fluorocarbons; D005780:Gelatin / Q000627:therapeutic use*; D011817:Rabbits; D012160:Retina / Q000502:physiology; D012163:Retinal Detachment / Q000175:diagnosis / Q000188:drug therapy* / Q000503:physiopathology; D012167:Retinal Perforations / Q000175:diagnosis / Q000188:drug therapy* / Q000503:physiopathology; D014014:Tissue Adhesives; D041623:Tomography, Optical Coherence; D011503:Transglutaminases / Q000627:therapeutic use*; D014821:Vitrectomy
chemical_list: D005466:Fluorocarbons; D014014:Tissue Adhesives; C073608:perfluorooctane; D005780:Gelatin; D011503:Transglutaminases