title: Crystallization and preliminary X-ray crystallographic analysis of UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase (VvGT5) from the grapevine Vitis vinifera.
abstract: Grapevine (Vitis vinifera) glycosyltransferase 5 (VvGT5) is a UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase that catalyses the 3-O-specific glucuronosylation of flavonols using UDP-glucuronic acid as a sugar donor to produce flavonol 3-O-glucosides, which are important bioactive phytochemicals. Recombinant VvGT5 expressed in Escherichia coli cells was purified and crystallized by the sitting-drop vapour-diffusion method. A full set of X-ray diffraction data was collected to 2.2 Å Bragg spacing from a single crystal using a synchrotron-radiation source. The crystal was hexagonal, belonging to space group P6(1)22, with unit-cell parameters a = b = 102.70, c = 535.92 Å. The initial phases were determined by the molecular-replacement method.
authors.affiliation: Division of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. mizohata@chem.eng.osaka-u.ac.jp
authors.forename: Eiichi
authors.initials: E
authors.lastname: Mizohata
authors.forename: Takuma
authors.initials: T
authors.lastname: Okuda
authors.forename: Seika
authors.initials: S
authors.lastname: Hatanaka
authors.forename: Taisuke
authors.initials: T
authors.lastname: Nakayama
authors.forename: Manabu
authors.initials: M
authors.lastname: Horikawa
authors.forename: Toru
authors.initials: T
authors.lastname: Nakayama
authors.forename: Eiichiro
authors.initials: E
authors.lastname: Ono
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inoue
mesh_terms: D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics; D005964:Glucosyltransferases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D027843:Vitis / Q000201:enzymology*
chemical_list: D010940:Plant Proteins; D011994:Recombinant Proteins; D005964:Glucosyltransferases; C023249:flavonol 3-O-glucosyltransferase