title: Electrophysiological response of chicken's jejunal epithelium to increasing levels of T-2 toxin.
abstract: The present investigations were conducted to test the effects of T-2 toxin on electrophysiological variables of jejunal epithelium of chicken. Jejunal segments of broilers were monitored in Ussing chambers in the presence of T-2 toxin at the levels of 0 (negative control), 0 (methanol/vehicle control), 0.1, 1, 5, and 10 μg/ml of buffer. T-2 toxin did not affect basal values of short circuit current (I(sc)), transmural potential difference, or tissue conductivity in the jejunal epithelium. T-2 toxin also did not statistically affect glucose-induced electrophysiological variables during the first 3 min of glucose induction. Compared to the vehicle control, the ouabain-sensitive I(sc) was negatively affected (P = 0.008) only under 5 μg of T-2 toxin/ml. Increasing levels of T-2 toxin negatively affected the ouabain-sensitive I(sc) in a cubic (P = 0.007) fashion. These data indicate that acute exposure to moderate levels of T-2 toxin may progressively impair the cation gradient across the jejunal epithelium.
authors.affiliation: Animal Nutrition Programme, Animal Sciences Institute, National Agricultural Research Centre, Park Road, 45500 Islamabad, Pakistan. aghawaqaryunus@yahoo.com
authors.forename: Agha Waqar
authors.initials: AW
authors.lastname: Yunus
authors.forename: Susan
authors.initials: S
authors.lastname: Kröger
authors.forename: Alexander
authors.initials: A
authors.lastname: Tichy
authors.forename: Jürgen
authors.initials: J
authors.lastname: Zentek
authors.forename: Josef
authors.initials: J
authors.lastname: Böhm
mesh_terms: D000818:Animals; D002412:Cations / Q000378:metabolism; D002645:Chickens; D055724:Electrophysiological Phenomena / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000502:physiology*; D007583:Jejunum / Q000187:drug effects* / Q000502:physiology*; D013605:T-2 Toxin / Q000633:toxicity*
chemical_list: D002412:Cations; D013605:T-2 Toxin