title: Tissue-specific downregulation of the adiponectin "system": possible implications for fat accumulation tendency in the pig.
abstract: Adiponectin's beneficial effects are mediated by the AdipoR1 and AdipoR2 receptors (AdipoRs). The pig is a good model to study complex disorders such as obesity. We analyzed the expression of adiponectin, AdipoRs and some key molecules of energy metabolism (AMP-activated protein kinase α [AMPKα], p38 mitogen-activated protein kinase [p38 MAPK], and PPARα) in 2 pig breeds that displayed an opposite genetic behavior for energy metabolism: Casertana (CE), a fat-type animal, and Large White (LW), a lean-type animal. Muscle, liver, visceral and subcutaneous adipose tissues, and brain tissues were examined. The AdipoRs cDNA sequences were identical in the 2 breeds. AdipoRs mRNA expression, measured in all tissues, was significantly lower only in the 2 adipose tissues of CE pigs (P < 0.05). The muscle expression of AdipoRs, AMPKα, p38 MAPK, and PPARα was lower in CE than in LW animals (P < 0.01, P < 0.05, P < 0.01, P < 0.01, respectively). In liver, no molecule differed between breeds. The expression of both AdipoRs in visceral and subcutaneous adipose tissues was lower in CE pigs (P < 0.01). In brain, AdipoR1 and AMPKα expression was lower in CE pigs (P < 0.01), whereas AdipoR2 tended to be lower in CE than LW pigs (P = 0.05). In conclusion, our results suggest that tissue-specific downregulation of Adiponectin, AdipoRs, and of the key molecules of energy metabolism may be associated with the tendency of CE pigs to accumulate fat.
authors.affiliation: CEINGE- Biotecnologie Avanzate Scarl, Via Gaetano Salvatore 482, 80145, Napoli, Italy.
authors.forename: A
authors.initials: A
authors.lastname: De Rosa
authors.forename: M L
authors.initials: ML
authors.lastname: Monaco
authors.forename: E
authors.initials: E
authors.lastname: Nigro
authors.forename: O
authors.initials: O
authors.lastname: Scudiero
authors.forename: M
authors.initials: M
authors.lastname: D'Andrea
authors.forename: F
authors.initials: F
authors.lastname: Pilla
authors.forename: G
authors.initials: G
authors.lastname: Oriani
authors.forename: A
authors.initials: A
authors.lastname: Daniele
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D052242:Adiponectin / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D015536:Down-Regulation; D004734:Energy Metabolism; D005260:Female; D008297:Male; D047493:PPAR alpha / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D054419:Receptors, Adiponectin / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D050151:Subcutaneous Fat / Q000378:metabolism*; D013552:Swine / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism
chemical_list: D052242:Adiponectin; D047493:PPAR alpha; D012333:RNA, Messenger; D054419:Receptors, Adiponectin; D048051:p38 Mitogen-Activated Protein Kinases; D055372:AMP-Activated Protein Kinases