title: Umbilical cord blood mesenchymal stem cells protect amyloid-β42 neurotoxicity via paracrine.
abstract: OBJECTIVE
  To understand the neuroprotective mechanism of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) against amyloid-β42 (Aβ42) exposed rat primary neurons.
  
  
  METHODS
  To evaluate the neuroprotective effect of hUCB-MSCs, the cells were co-cultured with Aβ42-exposed rat primary neuronal cells in a Transwell apparatus. To assess the involvement of soluble factors released from hUCB-MSCs in neuroprotection, an antibody-based array using co-cultured media was conducted. The neuroprotective roles of the identified hUCB-MSC proteins was assessed by treating recombinant proteins or specific small interfering RNAs (siRNAs) for each candidate protein in a co-culture system.
  
  
  RESULTS
  The hUCB-MSCs secreted elevated levels of decorin and progranulin when co-cultured with rat primary neuronal cells exposed to Aβ42. Treatment with recombinant decorin and progranulin protected from Aβ42-neurotoxicity in vitro. In addition, siRNA-mediated knock-down of decorin and progranulin production in hUCB-MSCs reduced the anti-apoptotic effects of hUCB-MSC in the co-culture system.
  
  
  CONCLUSIONS
  Decorin and progranulin may be involved in anti-apoptotic activity of hUCB-MSCs exposed to Aβ42.
authors.affiliation: Ju-Yeon Kim, Dong Hyun Kim, Ji Hyun Kim, Yoon Sun Yang, Wonil Oh, Jong Wook Chang, Biomedical Research Institute, R & D Center, MEDIPOST Co., Ltd. Seoul 137-874, South Korea.
authors.forename: Ju-Yeon
authors.initials: JY
authors.lastname: Kim
authors.forename: Dong Hyun
authors.initials: DH
authors.lastname: Kim
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Yoon Sun
authors.initials: YS
authors.lastname: Yang
authors.forename: Wonil
authors.initials: W
authors.lastname: Oh
authors.forename: Eun Hui
authors.initials: EH
authors.lastname: Lee
authors.forename: Jong Wook
authors.initials: JW
authors.lastname: Chang
keywords: Anti-apoptosis; Aβ42; Decorin; Human umbilical cord blood-derived mesenchymal stem cells; Progranulin