title: Ultrasound-induced new cellular mechanism involved in drug resistance.
abstract: The acoustic effects in a biological milieu offer several scenarios for the reversal of multidrug resistance. In this study, we have observed higher sensitivity of doxorubicin-resistant uterine sarcoma MES-SA/DX5 cells to ultrasound exposure compared to its parent counterpart MES-SA cells; however, the results showed that the acoustic irradiation was genotoxic and could promote neotic division in exposed cells that was more pronounced in the resistant variant. The neotic progeny, imaged microscopically 24 hr post sonication, could contribute in modulating the final cell survival when an apoptotic dose of doxorubicin was combined with ultrasound applied either simultaneously or sequentially in dual-treatment protocols. Depending on the time and order of application of ultrasound and doxorubicin in combination treatments, there was either desensitization of the parent cells or sensitization of the resistant cells to doxorubicin action.
authors.affiliation: Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan.
authors.forename: Mariame A
authors.initials: MA
authors.lastname: Hassan
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Furusawa
authors.forename: Masami
authors.initials: M
authors.lastname: Minemura
authors.forename: Natalya
authors.initials: N
authors.lastname: Rapoport
authors.forename: Toshiro
authors.initials: T
authors.lastname: Sugiyama
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
mesh_terms: D002453:Cell Cycle / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology; D018432:Drug Resistance, Multiple / Q000187:drug effects; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D006657:Histones / Q000378:metabolism; D006801:Humans; D010766:Phosphorylation / Q000187:drug effects; D013997:Time Factors; D014465:Ultrasonics
chemical_list: C468783:H2AX protein, human; D006657:Histones; D004317:Doxorubicin