title: [Alternation of thioredoxin system in postconditioning with hydrogen sulfide against hepatic ischemia-reperfusion injury in rats].
abstract: OBJECTIVE
  To explore the relationship between the protection of hydrogen sulfide (H(2)S) against hepatic ischemia-reperfusion injury and thioredoxin system in rats.
  
  
  METHODS
  Eighteen adult rats were divided randomly into 3 groups, i.e. sham, ischemia-reperfusion (IR) and sodium hydrogen sulfide (NaHS). The rats in the IR and NaHS groups were subjected to ischemia for 60 min and followed by reperfusion for 6 hours. In the NaHS group, there was an intraperitoneal dosing of NaHS (28 µmol/kg) at 5 min pre-reperfusion. Blood samples were collected for the measurements of alanine transaminase (ALT) and aspartate transaminase (AST). Liver tissue samples were collected for measurements of Trx and TrxR activity by enzyme-linked immunosorbent assay (ELISA), Western blot detection of Trx system proteins and thioredoxin interacting protein (TXNIP) expression. Hematoxylin-eosin staining was used to observe the hepatic histopathological changes.
  
  
  RESULTS
  Compared with the sham group, increased activities of ALT and AST were found in the IR group, accompanied by aggravated pathological injury. In addition, NaHS administrated at pre-reperfusion could alleviate hepatic injury. Compared with the sham group, the IR group had decreased Trx activity and Trx1 protein expression and increased TXNIP protein expression (P < 0.05) while the NaHS group had increased Trx activity and Trx1 protein expression and decreased TXNIP protein expression (P < 0.05). It indicated that the postconditioning with H(2)S could reduce the inhibition of Trx system and boost tissue antioxidant capacity.
  
  
  CONCLUSIONS
  Hydrogen sulfide postconditioning can enhance the cellular Trx system and play a protective role in hepatic ischemia-reperfusion injury.
authors.affiliation: Department of Anesthesiology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors.forename: Jin
authors.initials: J
authors.lastname: DU
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Qing-ming
authors.initials: QM
authors.lastname: Li
authors.forename: Bu-mei
authors.initials: BM
authors.lastname: Zhang
authors.forename: Ke-liang
authors.initials: KL
authors.lastname: Xie
authors.forename: Guo-lin
authors.initials: GL
authors.lastname: Wang
mesh_terms: D000818:Animals; D006862:Hydrogen Sulfide / Q000378:metabolism / Q000494:pharmacology*; D057775:Ischemic Postconditioning; D008099:Liver / Q000098:blood supply; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism*; D013879:Thioredoxins / Q000378:metabolism*
chemical_list: D013879:Thioredoxins; D006862:Hydrogen Sulfide