title: Duration of action of sustained-release buprenorphine in 2 strains of mice.
abstract: Buprenorphine HCl is a common analgesic for laboratory mice undergoing surgical procedures. The documented duration of action of buprenorphine HCl is as short as 3 to 5 h in mice, potentially necessitating readministration for continued analgesia. A long-acting buprenorphine formulation  would reduce handling-associated stress and provide uninterrupted analgesia. This study used the hot-plate assay to assess the antinociceptive effects of a single injection of sustained-release buprenorphine (bup-SR), buprenorphine-HCl (bup-HCl), and saline over 72 h in young adult male BALB/cJ  and SWR/J mice. SWR/J mice had shorter baseline latencies than did BALB/cJ mice, possibly reflecting greater sensitivity to thermal nociception. Relative increase from baseline latency (% maximal possible effect) was significant for buprenorphine-SR at 2, 6, and 12 h compared with saline.  According to results from a hot-plate assay, the analgesic efficacy of buprenorphine-SR appears to last at least 12 h in male BALB/cJ and SWR/J mice.
authors.affiliation: Laboratory Animal Resource Center, University of California San Francisco, San Francisco, California, USA. etcarbo@gmail.com
authors.forename: Elizabeth T
authors.initials: ET
authors.lastname: Carbone
authors.forename: Krista E
authors.initials: KE
authors.lastname: Lindstrom
authors.forename: Sandy
authors.initials: S
authors.lastname: Diep
authors.forename: Larry
authors.initials: L
authors.lastname: Carbone
mesh_terms: D000701:Analgesics, Opioid / Q000008:administration & dosage*; D000818:Animals; D002047:Buprenorphine / Q000008:administration & dosage*; D008297:Male; D051379:Mice / Q000145:classification; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D010146:Pain / Q000188:drug therapy*; D010147:Pain Measurement / Q000379:methods; D013045:Species Specificity
chemical_list: D000701:Analgesics, Opioid; D002047:Buprenorphine