title: Therapeutic efficacy of epigallocatechin gallate-loaded nanoliposomes against burn wound infection by methicillin-resistant Staphylococcus aureus.
abstract: OBJECTIVE
  Staphylococcus aureus remains a common cause of burn wound infection. Different studies have shown that the entrapment of plant-derived material such as epigallocatechin gallate (EGCG) in liposomes could increase their anti-S. aureus activity. The objectives of this study were to prepare EGCG-loaded nanoliposomes with variable surface charges and to evaluate their efficacy in vitro and in vivo against methicillin-resistant S. aureus (MRSA).
  
  
  METHODS
  EGCG-loaded nanoliposomes with positive, negative and neutral surface charges were prepared by extrusion method. Minimum inhibitory concentrations (MICs) of EGCG nanoliposomal forms against MRSA were determined by broth dilution method. The killing rates of the free and nanoliposomal forms of EGCG were analyzed. Ultimately, the in vivo therapeutic efficacy of the nanoliposomes in burned mouse skin infected by MRSA was investigated.
  
  
  RESULTS
  The MICs of the free, cationic, neutral and anionic nanoliposomal forms of EGCG against MRSA were 128, 16, 32 and 256 mg/l, respectively. The killing rates of the EGCG-loaded cationic nanoliposomes were higher than those of the other formulations. Treatment with the EGCG-loaded nanoliposomes with positive, neutral and negative surface charges resulted in almost 100, 70 and 30% survival rates, respectively.
  
  
  CONCLUSIONS
  The data suggested that the cationic EGCG-loaded nanoliposomes would be a good choice for the treatment of MRSA infections due to its high effectiveness.
authors.affiliation: Department of Laboratory Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran. amirgharib @ gmail.com
authors.forename: A
authors.initials: A
authors.lastname: Gharib
authors.forename: Z
authors.initials: Z
authors.lastname: Faezizadeh
authors.forename: M
authors.initials: M
authors.lastname: Godarzee
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002056:Burns / Q000188:drug therapy*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D007668:Kidney / Q000382:microbiology; D008081:Liposomes; D008099:Liver / Q000382:microbiology; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008826:Microbial Sensitivity Tests; D049329:Nanostructures; D012867:Skin / Q000382:microbiology; D013154:Spleen / Q000382:microbiology; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000382:microbiology; D014946:Wound Infection / Q000188:drug therapy* / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D008081:Liposomes; D002392:Catechin; C045651:epigallocatechin gallate