title: Quartz crystal microbalance with dissipation (QCM-D) as tool to exploit antigen-antibody interactions in pancreatic ductal adenocarcinomadetection.
abstract: Novel synthetic peptides represent smart molecules for antigen-antibody interactions in several bioanalytics applications, from purification to serum screening. Their immobilization onto a solid phase is considered a key point for sensitivity increasing. In this view, we exploited Quartz Crystal Microbalance with simultaneous frequency and dissipation monitoring (QCM-D) with a double aim, specifically, as investigative tool for spacers monolayer assembling and its functional evaluation, as well as high sensitive method for specific immunosorbent assays. The method was applied to pancreatic ductal adenocarcinoma (PDAC) detection by studying the interactions between synthetic phosphorylated and un-phosphorylated α-enolase peptides with sera of healthy and PDAC patients. The synthetic peptides were immobilized on the gold surface of the QCM-D sensor via a self-assembled alkanethiol monolayer. The presented experimental results can be applied to the development of surfaces less sensitive to non-specific interactions with the final target to suggest specific protocols for detecting PDAC markers with un-labeled biosensors.
authors.affiliation: NNL-Institute of Nanoscience-NANO, CNR, Via per Arnesano 16, Lecce I-73100, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Bianco
authors.forename: Alessandra
authors.initials: A
authors.lastname: Aloisi
authors.forename: Valentina
authors.initials: V
authors.lastname: Arima
authors.forename: Michela
authors.initials: M
authors.lastname: Capello
authors.forename: Sammy
authors.initials: S
authors.lastname: Ferri-Borgogno
authors.forename: Francesco
authors.initials: F
authors.lastname: Novelli
authors.forename: Stefano
authors.initials: S
authors.lastname: Leporatti
authors.forename: Rosaria
authors.initials: R
authors.lastname: Rinaldi
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry*; D000936:Antigen-Antibody Complex / Q000737:chemistry; D021441:Carcinoma, Pancreatic Ductal / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006801:Humans; D010455:Peptides / Q000097:blood / Q000302:isolation & purification*; D010751:Phosphopyruvate Hydratase / Q000097:blood / Q000302:isolation & purification*; D010766:Phosphorylation; D058525:Quartz Crystal Microbalance Techniques
chemical_list: D000936:Antigen-Antibody Complex; D010455:Peptides; D006046:Gold; D010751:Phosphopyruvate Hydratase