title: Pseudomonas fluorescens can induce and divert the human β-defensin-2 secretion in intestinal epithelial cells to enhance its virulence.
abstract: The effect of intestinal molecules produced by the host on the virulence of Pseudomonas fluorescens is poorly documented. In the present work, we evaluated the secretion of human β-defensin-2 (hBD-2) by enterocytes after infection with P. fluorescens (a species previously suggested to be involved in inflammatory bowel disease) and investigated the effect of this host-defense peptide on the bacterial virulence. The results showed that P. fluorescens can induce hBD-2 production in Caco-2/TC7 cells via P38 and ERK MAPK-dependent pathways. Surprisingly, the exposure of P. fluorescens to low doses of the antimicrobial peptide was found to enhance its cytotoxic and proinflammatory effects suggesting a potential feedback mechanism in the dialog between bacteria and the host.
authors.affiliation: Laboratoire de Microbiologie Signaux et Microenvironnement, EA4312, Université de Rouen, 55 rue Saint Germain, 27000 Evreux, France.
authors.forename: Amar
authors.initials: A
authors.lastname: Madi
authors.forename: Ziad
authors.initials: Z
authors.lastname: Alnabhani
authors.forename: Charlène
authors.initials: C
authors.lastname: Leneveu
authors.forename: Lily
authors.initials: L
authors.lastname: Mijouin
authors.forename: Marc
authors.initials: M
authors.lastname: Feuilloley
authors.forename: Nathalie
authors.initials: N
authors.lastname: Connil
mesh_terms: D000890:Anti-Infective Agents / Q000378:metabolism / Q000494:pharmacology; D018938:Caco-2 Cells; D020895:Enterocytes / Q000378:metabolism / Q000382:microbiology; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology*; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000382:microbiology; D020935:MAP Kinase Signaling System / Q000502:physiology; D016328:NF-kappa B / Q000378:metabolism; D011551:Pseudomonas fluorescens / Q000187:drug effects / Q000378:metabolism* / Q000472:pathogenicity*; D014774:Virulence / Q000502:physiology; D023083:beta-Defensins / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; C106641:DEFB4A protein, human; D016328:NF-kappa B; D023083:beta-Defensins