title: The Gcm/Glide molecular and cellular pathway: new actors and new lineages.
abstract: In Drosophila, the transcription factor Gcm/Glide plays a key role in cell fate determination and cellular differentiation. In light of its crucial biological impact, major efforts have been put for analyzing its properties as master regulator, from both structural and functional points of view. However, the lack of efficient antibodies specific to the Gcm/Glide protein precluded thorough analyses of its regulation and activity in vivo. In order to relieve such restraints, we designed an epitope-tagging approach to "FLAG"-recognize and analyze the functional protein both in vitro (exogenous Gcm/Glide) and in vivo (endogenous protein). We here (i) reveal a tight interconnection between the small RNA and the Gcm/Glide pathways. AGO1 and miR-1 are Gcm/Glide targets whereas miR-279 negatively controls Gcm/Glide expression (ii) identify a novel cell population, peritracheal cells, expressing and requiring Gcm/Glide. Peritracheal cells are non-neuronal neurosecretory cells that are essential in ecdysis. In addition to emphasizing the importance of following the distribution and the activity of endogenous proteins in vivo, this study provides new insights and a novel frame to understand the Gcm/Glide biology.
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch 67404, France.
authors.forename: Pietro
authors.initials: P
authors.lastname: Laneve
authors.forename: Claude
authors.initials: C
authors.lastname: Delaporte
authors.forename: Guillaume
authors.initials: G
authors.lastname: Trebuchet
authors.forename: Orban
authors.initials: O
authors.lastname: Komonyi
authors.forename: Hakima
authors.initials: H
authors.lastname: Flici
authors.forename: Anna
authors.initials: A
authors.lastname: Popkova
authors.forename: Giuseppe
authors.initials: G
authors.lastname: D'Agostino
authors.forename: Francesca
authors.initials: F
authors.lastname: Taglini
authors.forename: Irene
authors.initials: I
authors.lastname: Kerekes
authors.forename: Angela
authors.initials: A
authors.lastname: Giangrande
mesh_terms: D000818:Animals; D060565:Argonaute Proteins / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000196:embryology / Q000235:genetics / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D047108:Embryonic Development; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000235:genetics; D035683:MicroRNAs / Q000378:metabolism*; D018978:Molting; D009154:Mutation; D009420:Nervous System / Q000196:embryology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C489654:AGO1 protein, Drosophila; D060565:Argonaute Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C102805:GCM protein, Drosophila; C574927:MIRN1 microRNA, Drosophila; C568310:MIRN279 microRNA, Drosophila; D035683:MicroRNAs; D014157:Transcription Factors; D049452:Green Fluorescent Proteins