title: Hourly activity and natural infection of sandflies (Diptera: psychodidae) captured from the aphotic zone of a cave, minas gerais state, Brazil.
abstract: Sandflies are holometabolous insects that are of great epidemiological importance in the neotropical region as vectors of leishmaniases. Caves are ecotopes that significantly differ from external environments and, among the insects that live or visit their internal area and adjacent environment, sandflies are commonly found. Based on this context, the objective of this work was to examine the period of activity of sandflies in the cave environment in the aphotic zone. Thus, four sandfly captures were conducted, one in each season of the year, in a cave where studies on the bioecological aspects of sandfly fauna have been conducted since 2008. In this same study, we have also noticed the presence of flagellates in some captured females. Catches were carried out for 24 hours using a Shannon trap, light bait, and cave walls were actively searched. We collected a total of 638 sandflies, representing 11 species. The most abundant species and with more intense period of activity were, in descending order: Lu. cavernicola (62%), Ev. spelunca (16%) and Ev. sallesi (14%). A total of 69 females were dissected to check for natural infection, and in five specimens we found living flagellated forms: two Ev. spelunca, two Ev. sallesi and one Sc. sordellii. This study shows that the activity of some species caught in the aphotic zone of the cave, especially Lu. cavernicola, differs from what has already been reported in previous sandfly captures, which are almost always conducted at night and during twilight. The existence of sandflies that were naturally infected with flagellates and the lack of awareness regarding the behaviour of sandflies in cave environments are strong indicators of the need for further study on this group of insects in this ecotope, as a safety measure to protect the visitors of such environment.
authors.affiliation: Grupo de Estudos em Leishmanioses, Centro de Pesquisas René Rachou/Portuguese Fundação Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Gustavo Mayr de Lima
authors.initials: GM
authors.lastname: Carvalho
authors.forename: Reginaldo Peçanha
authors.initials: RP
authors.lastname: Brazil
authors.forename: Lara
authors.initials: L
authors.lastname: Saraiva
authors.forename: Patrícia Flávia
authors.initials: PF
authors.lastname: Quaresma
authors.forename: Helbert Antônio
authors.initials: HA
authors.lastname: Botelho
authors.forename: Mariana Campos das Neves Farah
authors.initials: MC
authors.lastname: Ramos
authors.forename: Ana Paula Lusardo
authors.initials: AP
authors.lastname: de Almeida Zenóbio
authors.forename: Paula Cavalcante Lamy Serra
authors.initials: PC
authors.lastname: e Meira
authors.forename: Cristiani
authors.initials: C
authors.lastname: de Castilho Sanguinette
authors.forename: José Dilermando Andrade
authors.initials: JD
authors.lastname: Filho
mesh_terms: D000818:Animals; D059552:Caves; D004175:Diptera / Q000502:physiology; D005260:Female; D008297:Male; D011576:Psychodidae / Q000502:physiology*