title: [Etiological detection of severe hand-food-mouth disease and related genetic characteristics of enterovirus type 71 infection in Beijing, 2010].
abstract: OBJECTIVE
  To study the etiological detection on samples from severe hand-foot-mouth disease (HFMD) cases and the genetic characteristics of enterovirus type 71 (EV71) isolates from severe patients in Beijing, 2010.
  
  
  METHODS
  Real-time RT-PCR was used to detect EV71 and Coxsackievirus A16 (CoxA16) and RD cells were used to separate virus strains from samples. Homogeneity of EV71 isolated strains were also analyzed.
  
  
  RESULTS
  Four hundred and fourty-two severe cases were detected and 253 were positive, taking up 57.24% of the total (253/442). The overall positive detection rate on EV71 was 54.55% (138/253), with CoxA16 as 5.93% (15/253), and with other enterovirus group was 39.53% (100/253). The nucleotide homogeneity of VP1 within these 12 strains was 97.2% - 100.0%, and with Beijing strains in 2007 - 2010, Shandong strains in 2007 and Anhui Fuyang strains in 2008 and the Guangdong strains in 2008 as 94.0% - 99.9%.
  
  
  CONCLUSIONS
  Severe HFMD cases were most oftenly caused by EV71 but less caused by CoxA16 or other enterovirus. The HFMD in 2010 in Beijing was mainly caused by EV71 subgenotype C4a with 4 transmission chains. Twelve isolated EV71 strains had high homogeneity with strains isolated from severe cases in Anhui Fuyang in 2007.
authors.affiliation: Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Control and Prevention, Beijing 100013, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Mei
authors.initials: M
authors.lastname: Qu
authors.forename: Lei
authors.initials: L
authors.lastname: Jia
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Bai-wei
authors.initials: BW
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Huang
authors.forename: Xin-yu
authors.initials: XY
authors.lastname: Li
authors.forename: Quan-yi
authors.initials: QY
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D029821:Enterovirus A, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D005260:Female; D006232:Hand, Foot and Mouth Disease / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D055815:Young Adult