title: Radiorespirometric assays for the detection of biogenic sulfides from sulfate-reducing bacteria.
abstract: OBJECTIVE
  The detection of trace concentrations of biogenic sulfides can be carried out through radiorespirometric assays. The objective of this work was to improve the methodology for detection of H2 S in trace concentrations, to correlate with sulfate-reducing bacterial activity.
  
  
  RESULTS
  Serial dilutions of synthetic sea water with a pure culture of Desulfovibrio alaskensis, a mixed anaerobic microbial culture and a natural saline sample from a petroleum offshore platform indicated that dilutions were followed, accordingly, by sulfate reduction.
  
  
  CONCLUSIONS
  Tests performed indicated that increasing the time of incubation of a mixed anaerobic microbial culture contributed to an increase in the sulfate reduction rates, as well as the amount of carbon source and inoculum.
  
  
  CONCLUSIONS
  The technique here developed proved to be a rapid test for the detection of biogenic sulfides, particularly those associated with corrosion products, being an useful tool for monitoring and controlling oil/water storage tanks, petroleum continental platforms and several types of reservoirs.
authors.affiliation: Instituto de Química, Programa de Pós-Graduação em Química, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brasil.
authors.forename: J C
authors.initials: JC
authors.lastname: de Queiroz
authors.forename: A C
authors.initials: AC
authors.lastname: de Melo Ferreira
authors.forename: A C A
authors.initials: AC
authors.lastname: da Costa
mesh_terms: D002244:Carbon / Q000378:metabolism; D003343:Corrosion; D003901:Desulfovibrio / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology*; D013431:Sulfates / Q000378:metabolism; D013440:Sulfides / Q000302:isolation & purification*; D016999:Sulfur-Reducing Bacteria / Q000378:metabolism*; D014871:Water Microbiology
chemical_list: D013431:Sulfates; D013440:Sulfides; D002244:Carbon