title: Analysis of survivin-specific T cells in breast cancer patients using human DCs engineered with survivin mRNA.
abstract: The observation that dendritic cells (DCs) charged with tumor-associated antigens (TAAs) is a potent strategy to elicit protective immunity in tumor-bearings hosts has prompted extensive testing of DCs as cellular adjuvant in cancer vaccines. To improve the clinical development of DC-based cancer vaccines, it may be beneficial to analyze preexistent immunity against TAAs in cancer patients because it may be easier to expand a memory pool of T cells compared to generating new immunity. Recent research shows that engineering DCs to synthesize tumor epitopes endogenously by transfecting DCs with mRNA-encoding TAAs are particular effective in stimulating robust T-responses in vitro and in vivo. In this chapter, we describe the methodology to analyze for survivin-specific T cells in breast cancer patients using human DCs engineered with survivin mRNA.
authors.affiliation: Department of Hematology, Center for Cancer Immune Therapy (CCIT), University Hospital Herlev, Copenhagen, Denmark. ozcan.met@regionh.dk
authors.forename: Ozcan
authors.initials: O
authors.lastname: Met
authors.forename: Inge Marie
authors.initials: IM
authors.lastname: Svane
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D019496:Cancer Vaccines / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D060846:Cell Engineering / Q000379:methods*; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D005260:Female; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D012333:RNA, Messenger / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D000077022:Survivin; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: C107489:BIRC5 protein, human; D019496:Cancer Vaccines; D018984:Epitopes, T-Lymphocyte; D051034:Inhibitor of Apoptosis Proteins; D012333:RNA, Messenger; D000077022:Survivin