title: [Sperm DNA damage and sperm-nucleoprotein transition correlate to acrosin activity and seminal parameters].
abstract: OBJECTIVE
  To investigate the correlation of sperm DNA damage and sperm-nucleoprotein transition with acrosin activity and seminal parameters.
  
  
  METHODS
  We collected 535 semen samples, assessed sperm DNA damage by sperm chromatin dispersion test, and analyzed the correlation of sperm DNA damage and sperm-nucleoprotein transition with acrosin activity and seminal parameters according to the WHO criteria.
  
  
  RESULTS
  Statistically significant differences were observed in sperm DNA damage among sperm-nucleoprotein transition, acrosin activity, sperm concentration and the percentage of grade a + b sperm (P < 0.01). Sperm DNA damage was positively correlated with age, sperm-nucleoprotein transition, sperm concentration and the percentage of grade d sperm (P < 0.01 or P < 0.05), but negatively correlated with acrosin activity (P < 0.001). Stepwise linear regression analysis demonstrated that age, sperm concentration, the percentage of grade d sperm, sperm-nucleoprotein transition and acrosin activity were independent variables related to the DNA fragmentation index (DFI). The abnormality rates of sperm-nucleoprotein transition, acrosin activity, sperm concentration and graded a + b sperm were significantly higher in the sperm DNA damage group (DFI > or = 30%) than in the normal control (DFI < 30%) (P < 0.01).
  
  
  CONCLUSIONS
  Sperm DNA damage is closely related with sperm-nucleoprotein transition, acrosin activity and seminal parameters, which may become another important independent parameter for the evaluation of sperm quality.
authors.affiliation: Reproductive Medicine Center, The First Hospital of Wenzhou Medical College, Wenzhou, Zhejiang 325000, China. zhengjiujia@126.com
authors.forename: Jiu-Jia
authors.initials: JJ
authors.lastname: Zheng
authors.forename: Xu
authors.initials: X
authors.lastname: Yang
authors.forename: Li-Ya
authors.initials: LY
authors.lastname: Zhang
authors.forename: Qian-Jin
authors.initials: QJ
authors.lastname: Fei
authors.forename: Cheng-Shuang
authors.initials: CS
authors.lastname: Pan
authors.forename: Wu-Hua
authors.initials: WH
authors.lastname: Ni
authors.forename: Jian-Yuan
authors.initials: JY
authors.lastname: Jin
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Huang
mesh_terms: D000176:Acrosin / Q000235:genetics*; D000328:Adult; D002843:Chromatin; D004249:DNA Damage; D053938:DNA Fragmentation; D006801:Humans; D007248:Infertility, Male / Q000235:genetics; D008297:Male; D009698:Nucleoproteins / Q000235:genetics / Q000378:metabolism*; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa
chemical_list: D002843:Chromatin; D009698:Nucleoproteins; D000176:Acrosin