title: A proteomic approach provides new insights into the control of soil-borne plant pathogens by Bacillus species.
abstract: Beneficial microorganisms (also known as biopesticides) are considered to be one of the most promising methods for more rational and safe crop management practices. We used Bacillus strains EU07, QST713 and FZB24, and investigated their inhibitory effect on Fusarium. Bacterial cell cultures, cell-free supernatants and volatiles displayed varying degrees of suppressive effect. Proteomic analysis of secreted proteins from EU07 and FZB24 revealed the presence of lytic enzymes, cellulases, proteases, 1,4-β-glucanase and hydrolases, all of which contribute to degradation of the pathogen cell wall. Further proteomic investigations showed that proteins involved in metabolism, protein folding, protein degradation, translation, recognition and signal transduction cascade play an important role in the control of Fusarium oxysporum. Our findings provide new knowledge on the mechanism of action of Bacillus species and insight into biocontrol mechanisms.
authors.affiliation: Department of Molecular Biology and Genetic, Faculty of Life Sciences, Muğla Sıtkı Koçman University, Muğla, Turkey. omurbaysal@mu.edu.tr
authors.forename: Omür
authors.initials: O
authors.lastname: Baysal
authors.forename: Duo
authors.initials: D
authors.lastname: Lai
authors.forename: Han-Hong
authors.initials: HH
authors.lastname: Xu
authors.forename: Mirko
authors.initials: M
authors.lastname: Siragusa
authors.forename: Mikail
authors.initials: M
authors.lastname: Calışkan
authors.forename: Francesco
authors.initials: F
authors.lastname: Carimi
authors.forename: Jaime A Teixeira
authors.initials: JA
authors.lastname: da Silva
authors.forename: Mahmut
authors.initials: M
authors.lastname: Tör
mesh_terms: D001407:Bacillus / Q000378:metabolism*; D001681:Biological Assay; D061046:Biological Control Agents; D005670:Fusarium / Q000378:metabolism; D014644:Genetic Variation; D005838:Genotype; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control*; D017510:Protein Folding; D040901:Proteomics / Q000379:methods*; D012988:Soil Microbiology; D013045:Species Specificity
chemical_list: D061046:Biological Control Agents