title: Dynamized follicle-stimulating hormone affects the development of ovine preantral follicles cultured in vitro.
abstract: OBJECTIVE
  To evaluate the effect of dynamized follicle-stimulating hormone (FSH) on the survival, activation and growth of ovine preantral follicles (PFs) in vitro.
  
  
  METHODS
  Ovarian fragments were cultured for 1 or 7 days in alpha minimum essential medium (α-MEM(+)) control in the absence or presence of alcohol (Al control) or FSH (6cH, 12cH and 30cH) added at intervals of 24 or 48 h. The ovarian fragments were processed, coded and analyzed by a blinded observer by classical histology (CH), fluorescence microscopy (FM) and transmission electron microscopy (TEM).
  
  
  RESULTS
  After 7 days of culture, the group which to which FSH 6cH was added at 24 h intervals showed better rates of follicle survival and activation compared to α-MEM(+) control or Al control (p < 0.05). This group also showed higher follicle and oocyte growth than α-MEM(+) control (p < 0.05). FM and TEM techniques confirmed that FSH 6cH promoted viability and ultrastructural integrity of follicles after 7 days of culture.
  
  
  CONCLUSIONS
  FSH 6cH (24 h) treatment maintained the viability, and promoted the activation and in vitro growth of ovine PFs.
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza-CE, Brazil. laritza_lima@yahoo.com.br
authors.forename: Laritza Ferreira de
authors.initials: LF
authors.lastname: Lima
authors.forename: Rebeca Magalhães Pedrosa
authors.initials: RM
authors.lastname: Rocha
authors.forename: Anelise Maria Costa Vasconcelos
authors.initials: AM
authors.lastname: Alves
authors.forename: Márcia Viviane Alves
authors.initials: MV
authors.lastname: Saraiva
authors.forename: Valdevane Rocha
authors.initials: VR
authors.lastname: Araújo
authors.forename: Isadora Machado Teixeira
authors.initials: IM
authors.lastname: Lima
authors.forename: Cláudio Afonso Pinho
authors.initials: CA
authors.lastname: Lopes
authors.forename: Sônia Nair
authors.initials: SN
authors.lastname: Báo
authors.forename: Claudio Cabral
authors.initials: CC
authors.lastname: Campello
authors.forename: Ana Paula Ribeiro
authors.initials: AP
authors.lastname: Rodrigues
authors.forename: José Ricardo de
authors.initials: JR
authors.lastname: Figueiredo
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002470:Cell Survival; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D006080:Ovarian Follicle / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D012756:Sheep
chemical_list: D005640:Follicle Stimulating Hormone