title: Comparative analysis of three Callicarpa herbs using high performance liquid chromatography with diode array detector and electrospray ionization-trap mass spectrometry method.
abstract: Three Callicarpa species, namely Callicarpa nudiflora Hook. et Arn., Callicarpa macrophylla Vahl. and Callicarpa kwangtungensis Chun. are astringency and hemostasis herbs in the traditional Chinese medical systems. Despite their wide use in Chinese medicine, no report on system comparison on their chemical constituents is available so far. High-performance liquid chromatography coupled with diode array detector and electrospray ionization trap mass spectrometry (HPLC-DAD-ESI-Trap MS) technique was used for qualitative and quantitative analyses of the three Callicarpa herbs. Phenylpropanoid glycosides, flavonoids and organic acids were identified by comparing with reference standards or according to their MS/MS fragmentation behaviors. A total of 33 compounds were identified identified or tentatively identified, and 23 of them were reported from these herbs for the first time. Phenylpropanoid glycosides were featured in the three species with their types and contents presenting significant differences. Furthermore, quantitative analysis was conducted by determining four marker phenylpropanoid glycosides (forsythoside B (14), acteoside (15), poliumoside (19), isoacteoside (21)) and two flavonoids (luteolin (30), apigenin (32)). Three flavonoid glucuronides (luteolin-diglucuronide-glucuronide (5), luteolin-diglucuronide (12), apigenin-7-O-β-glucuronide (24)) were semi-quantified according to their corresponding aglycones. The total contents of the nine major compounds in the three species varied significantly from 8.92 to 40.89 mg/g.
authors.affiliation: Department of Natural Medicine Chemistry, China Pharmaceutical University, Nanjing, China.
authors.forename: Yatao
authors.initials: Y
authors.lastname: Shi
authors.forename: Chunyong
authors.initials: C
authors.lastname: Wu
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Chen
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Feng
authors.forename: Ning
authors.initials: N
authors.lastname: Xie
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis / Q000737:chemistry; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis / Q000737:chemistry; D001252:Astringents / Q000032:analysis / Q000737:chemistry*; D002138:Calibration; D032408:Callicarpa / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D055664:Electrochemical Techniques; D020834:Ethnopharmacology; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry; D006027:Glycosides / Q000032:analysis / Q000737:chemistry; D006490:Hemostatics / Q000032:analysis / Q000737:chemistry*; D057230:Limit of Detection; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D000890:Anti-Infective Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D001252:Astringents; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D006027:Glycosides; D006490:Hemostatics; D010636:Phenols