title: Antioxidative, antiapoptotic, and proliferative effect of curcumin on liver regeneration after partial hepatectomy in rats.
abstract: The aim of the present study was to assess the influence of curcumin on liver regeneration after partial hepatectomy (PH) in rats. A total of 24 male Sprague Dawley rats were divided into three groups: sham-operated (SH), PH, and PH + curcumin; each group contains eight animals. The rats in curcumin-treated groups were given curcumin (in a dose of 100 mg/kg body weight) once a day orally for 7 days, starting 3 days prior to hepatectomy operation. At 7 days after resection, liver samples were collected. The malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione (GSH) levels were estimated in liver homogenates. Moreover, histopathological examination, mitotic index (MI), proliferating cell nuclear antigen labeling, proliferation index (PI), transferase-mediated 2'-deoxyuridine, 5'-triphosphate nick end-labeling assay, and apoptotic index (AI) were evaluated at 7 days after hepatectomy. As a result, curcumin significantly increased MI and PI and significantly decreased AI in PH rats. Additionally, curcumin remarkably inhibited MDA elevation, restored impaired antioxidant SOD activity and GSH level and also attenuated hepatic vacuolar degeneration and sinusoidal congestion. These results suggested that curcumin treatment had a beneficial effect on liver regenerative capacity of the remnant liver tissue after hepatectomy, probably due to its antioxidative, antiapoptotic, and proliferative properties.
authors.affiliation: Department of General Surgery, Istanbul Surgery Hospital, Istanbul, Turkey.
authors.forename: Toygar
authors.initials: T
authors.lastname: Toydemir
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Istanbul Medeniyet University, Istanbul, Turkey mkanter65@yahoo.com.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Kanter
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Namik Kemal University, Tekirdag, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Erboga
authors.affiliation: Department of General Surgery, Faculty of Medicine, Trakya University, Edirne, Turkey.
authors.forename: Serhat
authors.initials: S
authors.lastname: Oguz
authors.affiliation: Department of General Surgery, Faculty of Medicine, Trakya University, Edirne, Turkey.
authors.forename: Cengiz
authors.initials: C
authors.lastname: Erenoglu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D003474:Curcumin / Q000494:pharmacology*; D006498:Hepatectomy; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D003474:Curcumin
keywords: Curcumin; TUNEL; immunohistochemistry; oxidative stress; partial hepatectomy; rat