title: Identification and characterization of Klebsiella pneumoniae aldehyde dehydrogenases increasing production of 3-hydroxypropionic acid from glycerol.
abstract: Klebsiella pneumoniae produces 3-hydroxypropionic acid (3-HP) from glycerol with oxidation of 3-hydroxypropionaldehyde (3-HPA) to 3-HP in a reaction catalyzed by aldehyde dehydrogenase (ALDH). In the present study, two putative ALDHs of K. pneumoniae, YneI and YdcW were identified and characterized. Recombinant YneI was specifically active on 3-HPA and preferred NAD(+) as a cofactor, whereas YdcW exhibited broad substrate specificity and preferred NADP(+) as a cofactor. Overexpression of ALDHs in the glycerol oxidative pathway-deficient mutant K. pneumoniae AK resulted in a significant increase in 3-HP production upon shake-flask culture. The final titers of 3-HP were 2.4 and 1.8 g L(-1) by recombinants overexpressing YneI and YdcW, respectively. Deletion of the ALDH gene from K. pneumoniae did not affect the extent of 3-HP synthesis, implying non-specific activity of ALDHs on 3-HPA. The ALDHs might play major roles in detoxifying the aldehyde generated in glycerol metabolism.
authors.affiliation: Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology KRIBB, Jeongeup-si 580-185, South Korea.
authors.forename: Lian Hua
authors.initials: LH
authors.lastname: Luo
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Seo
authors.forename: Sun-Yeon
authors.initials: SY
authors.lastname: Heo
authors.forename: Baek-Rock
authors.initials: BR
authors.lastname: Oh
authors.forename: Dae-Hyuk
authors.initials: DH
authors.lastname: Kim
authors.forename: Chul Ho
authors.initials: CH
authors.lastname: Kim
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003067:Coenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005990:Glycerol / Q000737:chemistry* / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics; D019344:Lactic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D009243:NAD / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009249:NADP / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D003067:Coenzymes; D011994:Recombinant Proteins; D009243:NAD; D019344:Lactic Acid; D009249:NADP; C031601:hydracrylic acid; D000444:Aldehyde Dehydrogenase; D005990:Glycerol