title: Numerical evaluation of the limit of concentration of colloidal samples for their study with digital lensless holographic microscopy.
abstract: The number of colloidal particles per unit of volume that can be imaged correctly with digital lensless holographic microscopy (DLHM) is determined numerically. Typical in-line DLHM holograms with controlled concentration are modeled and reconstructed numerically. By quantifying the ratio of the retrieved particles from the reconstructed hologram to the number of the seeding particles in the modeled intensity, the limit of concentration of the colloidal suspensions up to which DLHM can operate successfully is found numerically. A new shadow density parameter for spherical illumination is defined. The limit of performance of DLHM is determined from a graph of the shadow density versus the efficiency of the microscope.
authors.affiliation: Universidad Nacional de Colombia, Sede Medellín, School of Physics, A.A: 3840, Medellín 050034, Colombia.
authors.forename: John F
authors.initials: JF
authors.lastname: Restrepo
authors.forename: Jorge
authors.initials: J
authors.lastname: Garcia-Sucerquia
mesh_terms: D003102:Colloids / Q000032:analysis* / Q000737:chemistry*; D006696:Holography / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D007909:Lenses; D053758:Nanoparticles / Q000032:analysis* / Q000737:chemistry / Q000648:ultrastructure; D009716:Numerical Analysis, Computer-Assisted; D010316:Particle Size; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted
chemical_list: D003102:Colloids