title: Genome-wide RNAi screening to identify regulators of oncogene-induced cellular senescence.
abstract: RNA interference (RNAi) is a powerful research tool that can be used to turn off-or silence-the expression of a specific gene. In recent years, RNAi screening on a genome-wide scale has provided the opportunity to identify factors and pathways involved in complex biological processes in a systematic and unbiased manner. Here we describe a genome-wide RNAi screening strategy to identify genes that are required for a particular type of cellular senescence called oncogene-induced senescence. The approach we describe is a general screening strategy that can be applied to the study of other forms of cellular senescence, including replicative senescence.
authors.affiliation: Department of Pathology, Yale University School of Medicine, New Haven, CT, USA.
authors.forename: Narendra
authors.initials: N
authors.lastname: Wajapeyee
authors.forename: Sara K
authors.initials: SK
authors.lastname: Deibler
authors.forename: Michael R
authors.initials: MR
authors.lastname: Green
mesh_terms: D000818:Animals; D002460:Cell Line; D002469:Cell Separation; D016922:Cellular Senescence / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D006801:Humans; D051379:Mice; D009857:Oncogenes / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D012190:Retroviridae / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D034741:RNA, Small Interfering