title: Virgibacillus natechei sp. nov., a moderately halophilic bacterium isolated from sediment of a saline lake in southwest of Algeria.
abstract: A novel, Gram-positive, moderately halophilic bacterium, oxidase- and catalase-positive designated FarD(T) was isolated from sediments of a saline lake located in Taghit, 93 km from Bechar, southwest of Algeria. Cells were rod-shaped, endospore forming, and motile. Growth occurred at 15-40 °C (optimum, 35 °C), pH 6.0-12.0 (optimum, 7.0) and in the presence of 1-20 % NaCl (optimum, 10 %). Strain FarD(T) used glucose, mannitol, melibiose, D-mannose, and 5 ketogluconate. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, and three phospholipids; MK-7 is the predominant menaquinone. The predominant cellular fatty acids were anteiso C15:0, anteiso C17:0, C20:0, and anteiso C19:0. The DNA G+C content was 42.1 mol%. Phylogenetic analysis of the small-subunit ribosomal RNA gene sequence indicated that strain FarD(T) had as its closest relative Virgibacillus salinus (similarity of 96.3 %). Based on phenotypic, phylogenetic, and taxonomic characteristics, strain FarD(T) is proposed as a novel species of the genus Virgibacillus within the order Clostridiales, for which the name V. natechei is proposed. The type strain is FarD(T) (=DSM 25609(T) = CCUG 62224(T)).
authors.affiliation: Microbiology Group, Laboratory of Cellular and Molecular Biology, Faculty of Biological Sciences, USTHB, BP 32, EL ALIA, Bab Ezzouar, Algiers, Algeria.
authors.forename: Meriam
authors.initials: M
authors.lastname: Amziane
authors.forename: Farida
authors.initials: F
authors.lastname: Metiaz
authors.forename: Amel
authors.initials: A
authors.lastname: Darenfed-Bouanane
authors.forename: Zahia
authors.initials: Z
authors.lastname: Djenane
authors.forename: Okba
authors.initials: O
authors.lastname: Selama
authors.forename: Ahmed
authors.initials: A
authors.lastname: Abderrahmani
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Cayol
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fardeau
mesh_terms: D000462:Algeria; D005227:Fatty Acids / Q000737:chemistry; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D058679:Virgibacillus / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S