title: Structural analysis of sulfated fucan from Saccharina japonica by electrospray ionization tandem mass spectrometry.
abstract: Desulfation of a fucoidan from Saccharina japonica by treatment with DMSO-MeOH resulted in partial degradation of polymeric molecules by methanolysis giving rise to a mixture of neutral, monosulfated, and disulfated fucooligosaccharides in the form of methyl glycosides. These oligomeric fragments were characterized by ESI-MS and ESI-CID-MS/MS. It was found that oligosaccharide structures coincided with the polysaccharide backbone built up mainly of (1→3)-linked fucose residues sulfated at positions 4 and 2.
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Jin
authors.forename: Zhimou
authors.initials: Z
authors.lastname: Guo
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Wenjing
authors.initials: W
authors.lastname: Zhang
authors.forename: Quanbin
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000459:Phaeophyceae / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D011134:Polysaccharides; C007789:fucoidan