title: [Gene clone and expression of Barx1 in different tooth of the mini-pig at embryonic day 40].
abstract: OBJECTIVE
  To partially clone and compare the quantitative expression of tooth development-related gene Barx1 in different teeth of the mini-pig embryo at embryonic day 40, and to investigate the relationship between Barx1 spatial quantitative expression and tooth morphogenesis.
  
  
  METHODS
  The mini-pig Barx1 genes was partially cloned and the mRNA sequences of human Barx1 genes was aligned with expressed sequence tags (EST) of pig by basic local alignment search tool (BLAST), which were assembled with DNAman v5.2.2. With designed primers, Barx1 was partially cloned in use of reverse transcription polymerase chain reaction (PCR), and tested by BLAST with all the species in NCBI database and confirmed as one part of target gene. Laser capture microdissection was used to collect tooth samples from frozen sections which were prepared before in -80°C freezer. Real-time PCR was carried out to analyze quantitative expression in different teeth.
  
  
  RESULTS
  Partial mini-pig Barx1 gene of 698 bp was cloned. Real-time PCR showed that, glyceraldehyde-3-phosphate dehydrogenase used as loading control, the figures of 2(-ΔCT) of lower deciduous incisor, canine, the third premolar and molar were 0.000 249, 0.000 715, 0.026 096 and 0.112 656, respectively. There was a trend of increasing expression from anterior to posterior teeth.
  
  
  CONCLUSIONS
  Barx1 gene could be related to the number or differentiation of tooth cusps.
authors.affiliation: Department of Orthodontics, Capital Medical University School of Stomatology, Beijing 100050, China. yangkai_cn@hotmail.com
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ji-rong
authors.initials: JR
authors.lastname: Yin
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
mesh_terms: D000818:Animals; D001641:Bicuspid / Q000378:metabolism; D003001:Cloning, Molecular; D003481:Cuspid / Q000378:metabolism; D004622:Embryo, Mammalian; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007180:Incisor / Q000378:metabolism; D008964:Molar, Third / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013552:Swine; D013556:Swine, Miniature; D014070:Tooth / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C490667:BARX1 protein, human; D018398:Homeodomain Proteins; D012333:RNA, Messenger; D014157:Transcription Factors