title: [Biodiversity of culturable halotolerant and halophilic bacteria isolated from saline-alkaline soils in Songnen Plain].
abstract: OBJECTIVE
  To analyze the biodiversity of halotolerant and halophilic bacteria the bacteria in saline-alkaline soils in Songnen Plain, we isolated and purified bacteria samples in the area.
  
  
  METHODS
  Halotolerant and halophilic bacteria were isolated from the enriched cultures of the saline-alkline soil samples through the traditional culture method, and 16S rRNA sequences were amplified and analyzed for the determination of phylogenetic relationships.
  
  
  RESULTS
  Forty strains were obtained and classified into 34 species, 16 genera, 8 families, 3 phylum (Firmicutes, gamma-Proteobacteria and Actinobacteria) in the Domain Bacteria. The genus Staphylococcus is the predominant group, followed by Halomonas, Oceanbacillus, Bacillus, Kocuria and Pseudomonas. The 16S rRNA sequences of 9 strains showed 97.2 to 99.0% similarities with their closest type strains, suggesting that they may be the potential novel species. The salt tolerance of the strains is mainly concentrated on the 5% - 10% NaCl while alkaline pH resistance of the strains is between pH 9 and 12. Based on the NaCl dependence, 62.5% of the strains were grouped into the halotolerant bacteria, and the rest into the moderate halophiles.
  
  
  CONCLUSIONS
  The halotolerant and halophilic bacteria are very rich in the saline-alkaline soils in Songnen Plain, and the genera Staphylococcus and Halomonas are the predominant groups. Almost all the bacteria from this area could tolerate not only at the high concentration of NaCl, but also at high alkaline pH. More importantly, a number of novel species may be included in this area.
authors.affiliation: Key Laboratory of Soybean Biology of Ministry of Education; Microbiology and Biotechnology, College of Life Sciences, Northeast Agricultural University, Harbin 150030, China. xinyuanfuture@136.com
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Pan
authors.forename: Haipeng
authors.initials: H
authors.lastname: Huang
authors.forename: Jing
authors.initials: J
authors.lastname: Meng
authors.forename: Hongyu
authors.initials: H
authors.lastname: Xiao
authors.forename: Cheng
authors.initials: C
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Meng
authors.forename: Shan
authors.initials: S
authors.lastname: Hong
authors.forename: Henan
authors.initials: H
authors.lastname: Liu
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Wang
authors.forename: Juquan
authors.initials: J
authors.lastname: Jiang
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D044822:Biodiversity; D002681:China; D008969:Molecular Sequence Data; D010802:Phylogeny; D012965:Sodium Chloride / Q000378:metabolism*; D012988:Soil Microbiology
chemical_list: D012965:Sodium Chloride