title: A simple and selective detection method for aristolochic acid in crude drugs using solid-phase extraction.
abstract: The official Japanese method for analyzing aristolochic acid I (AA-I) in Asiasarum root using conventional high-performance liquid chromatography (HPLC) is described in the Japanese Pharmacopoeia, Sixteenth Edition. Interfering peaks of AA-I sometimes appear after HPLC analysis of crude drugs. A selective analytical method is needed to determine definitively whether AA-I is present in crude drugs. In this study, we developed a selective method that combined solid-phase extraction and liquid chromatography/mass spectrometry (LC/MS) which may be useful for identifying AA-I in crude drugs and for quality control.
authors.affiliation: Osaka Prefectural Institute of Public Health, 1-3-69 Nakamichi, Higashinari-ku, Osaka, 537-0025, Japan, tagami@iph.pref.osaka.jp.
authors.forename: Takaomi
authors.initials: T
authors.lastname: Tagami
authors.forename: Akihiro
authors.initials: A
authors.lastname: Takeda
authors.forename: Akiko
authors.initials: A
authors.lastname: Asada
authors.forename: Airin
authors.initials: A
authors.lastname: Aoyama
authors.forename: Takahiro
authors.initials: T
authors.lastname: Doi
authors.forename: Masami
authors.initials: M
authors.lastname: Kawaguchi
authors.forename: Keiji
authors.initials: K
authors.lastname: Kajimura
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Sawabe
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Obana
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Yamasaki
mesh_terms: D034341:Aristolochic Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D020939:Zingiber officinale / Q000737:chemistry; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry; D052616:Solid Phase Extraction
chemical_list: D034341:Aristolochic Acids; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C000228:aristolochic acid I