title: SiO₂ nanoparticles as platform for delivery of nucleoside triphosphate analogues into cells.
abstract: A system for delivery of analogues of 2'-deoxyribonucleoside triphosphate (dNTP) based on SiO(2) nanoparticles was proposed. A simple and versatile method was developed for the preparation of SiO(2)-dNTP conjugates using the 'click'-reaction between premodified nanoparticles containing the azido groups and dNTP containing the alkyne-modified γ-phosphate group. The substrate properties of SiO(2)-dNTP were tested using Klenow fragment and HIV reverse transcriptase. Nucleoside triphosphates being a part of the SiO(2)-dNTP nanocomposites were shown to be incorporated into the growing DNA chain. The rate of polymerization with the use of SiO(2)-dNTP or common dNTP in case of HIV reverse transcriptase differed insignificantly. It was shown by confocal microscopy that the proposed SiO(2)-dNTP nanocomposites bearing the fluorescent label penetrate into cells and even into cellular nuclei.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Lavrent'ev Ave., 8, Novosibirsk 630090, Russia. svetlana2001@gmail.com
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Vasilyeva
authors.forename: Vladimir N
authors.initials: VN
authors.lastname: Silnikov
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Shatskaya
authors.forename: Asya S
authors.initials: AS
authors.lastname: Levina
authors.forename: Marina N
authors.initials: MN
authors.lastname: Repkova
authors.forename: Valentina F
authors.initials: VF
authors.lastname: Zarytova
mesh_terms: D003854:Deoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D016503:Drug Delivery Systems; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D058105:Polymerization; D011122:Polyphosphates / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D003854:Deoxyribonucleotides; D011122:Polyphosphates; D012822:Silicon Dioxide; C005692:triphosphoric acid