title: Metabolic responses of primary and transformed cells to intracellular Listeria monocytogenes.
abstract: The metabolic response of host cells, in particular of primary mammalian cells, to bacterial infections is poorly understood. Here, we compare the carbon metabolism of primary mouse macrophages and of established J774A.1 cells upon Listeria monocytogenes infection using (13)C-labelled glucose or glutamine as carbon tracers. The (13)C-profiles of protein-derived amino acids from labelled host cells and intracellular L. monocytogenes identified active metabolic pathways in the different cell types. In the primary cells, infection with live L. monocytogenes increased glycolytic activity and enhanced flux of pyruvate into the TCA cycle via pyruvate dehydrogenase and pyruvate carboxylase, while in J774A.1 cells the already high glycolytic and glutaminolytic activities hardly changed upon infection. The carbon metabolism of intracellular L. monocytogenes was similar in both host cells. Taken together, the data suggest that efficient listerial replication in the cytosol of the host cells mainly depends on the glycolytic activity of the hosts.
authors.affiliation: Lehrstuhl für Biochemie, Technische Universität München, Garching, Germany.
authors.forename: Nadine
authors.initials: N
authors.lastname: Gillmaier
authors.forename: Andreas
authors.initials: A
authors.lastname: Götz
authors.forename: Anette
authors.initials: A
authors.lastname: Schulz
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Eisenreich
authors.forename: Werner
authors.initials: W
authors.lastname: Goebel
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002244:Carbon / Q000378:metabolism; D002247:Carbon Isotopes; D002461:Cell Line, Transformed; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D042541:Intracellular Space / Q000187:drug effects / Q000382:microbiology*; D008089:Listeria monocytogenes / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D008088:Listeriosis / Q000382:microbiology / Q000473:pathology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology* / Q000473:pathology; D055442:Metabolome / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D000596:Amino Acids; D002247:Carbon Isotopes; D002244:Carbon; D005947:Glucose