title: Isolation and characterization of Pseudomonas sp. strain capable of degrading diethylstilbestrol.
abstract: Since diethylstilbestrol (DES) interrupts endocrine systems and generates reproductive abnormalities in both wildlife and human beings, methods to remove DES from the environments are urgently recommended. In this study, bacterial strain J51 was isolated and tested to effectively degrade DES. J51 was identified as Pseudomonas sp. based on its nucleotide sequence of 16S rRNA. The quinoprotein alcohol dehydrogenase and isocitrate lyase were identified to be involved in DES degradation by MALDI-TOF-TOF MS/MS analysis. In the presence of 40 mg/l DES, increase of the genes encoding quinoprotein alcohol dehydrogenase and isocitrate lyase in both RNA and protein levels was determined. The HPLC/MS analysis showed that DES was hydrolyzed to a major degrading metabolite DES-4-semiquinone. It was the first time to demonstrate the characteristics of DES degradation by specific bacterial strain and the higher degradation efficiency indicated the potential application of Pseudomonas sp. strain J51 in the treatment of DES-contaminated freshwater and seawater environments.
authors.affiliation: Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai, Shandong 264003, People's Republic of China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhang
authors.forename: Zongliang
authors.initials: Z
authors.lastname: Niu
authors.forename: Chunyang
authors.initials: C
authors.lastname: Liao
authors.forename: Lingxin
authors.initials: L
authors.lastname: Chen
mesh_terms: D004054:Diethylstilbestrol / Q000378:metabolism*; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D012336:RNA, Ribosomal, 16S; D004054:Diethylstilbestrol