title: Optimization of the nutrition for biodegradation of vinasse by Aspergillus oryzae using response surface methodology.
abstract: Direct discharge of vinasse from the distillery industry causes resource wasting and environmental destruction due to its mass of organic components. Aspergillus oryzae CGMCC5992 is capable of degrading the organic substrates of wastewater. One-factor-at-a-time design was adopted to select the most important nutrients influencing the degradation of organic materials of vinasse. Box-Behnken Design (BBD) with Design-Expert (8.0.4) was used to develop mathematical model equations, study responses, and optimize concentrations of the key nutrients to improve the degradation efficiency. The optimized medium containing 0.3 g/L urea, 20.73 mg/L ZnSO(4), and 19.79 mg/L vitamin B(6) was supplied to 10-times diluted vinasse; under the optimal condition, a decrease of chemical oxygen demand (COD) from 4,635 to 323 mg/L in vinasse was achieved in 5 days. The reduction of vinasse COD after the optimization of nutrient condition in this study is more significant than those reported previously.
authors.affiliation: School of Food Science and Biological Engineering, Jiangsu University, Zhenjiang, China. zhangzhicai2001@yahoo.com.cn
authors.forename: Zhicai
authors.initials: Z
authors.lastname: Zhang
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.forename: Fan
authors.initials: F
authors.lastname: Feng
authors.forename: Jiashao
authors.initials: J
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Qiaoxia
authors.initials: Q
authors.lastname: Pang
authors.forename: Keping
authors.initials: K
authors.lastname: Chen
mesh_terms: D001236:Aspergillus oryzae / Q000378:metabolism*; D001673:Biodegradation, Environmental; D057919:Biological Oxygen Demand Analysis; D007220:Industrial Waste; D007287:Inorganic Chemicals / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D009930:Organic Chemicals / Q000378:metabolism*; D025101:Vitamin B 6 / Q000378:metabolism; D014876:Water Pollution / Q000517:prevention & control
chemical_list: D007220:Industrial Waste; D007287:Inorganic Chemicals; D009930:Organic Chemicals; D025101:Vitamin B 6; D009584:Nitrogen