title: [Analysis of biological properties of strains-producers of a number of Pseudomonas aeruginosa recombinant proteins].
abstract: OBJECTIVE
  Study of biological properties of strains-producers of a number of Pseudomonas aeruginosa recombinant proteins compared with the initial strains.
  
  
  METHODS
  5 Escherichia coli strains (initial strains E. coli M15 and E. coli BL21 (DE3) and strains-producers of OprL, OprF, aTox3 recombinant proteins (E. coli M15/oprF, E. coli M 15/oprL, E. coli BL21-aTox3) were studied. Toxicity, toxigenicity and virulence determination were carried out in experiments in non-linear mice. Enzymatic properties of the initial strains and strains-producers were compared in a number of biochemical tests.
  
  
  RESULTS
  The studied strains-producers of the most immunogenic P. aeruginosa recombinant proteins were confirmed to be biosafe, belong to Enterobacteriaceae family and Escherichia genus. As a result of genetic engineering manipulations carried out with E. coli M15 and E. coli BL21(DE3) alterations ofbiochemical and growth properties, virulence, toxicity and toxigenicity in the constructed strains-producers of recombinant proteins - E. coli M 15/oprF, E. coli M15/oprL, E. coli BL21-aTox3 - were not detected.
  
  
  CONCLUSIONS
  The results obtained allow to consider the possibility of use of E. coli M 15/oprF, E. coli M15/oprL and E. coli BL21-aTox3 strains-producers for production and isolation of candidate proteins for inclusion into vaccine against pseudomonas infection.
authors.forename: A V
authors.initials: AV
authors.lastname: Soldatenkova
authors.forename: E I
authors.initials: EI
authors.lastname: Leonova
authors.forename: S A
authors.initials: SA
authors.lastname: Zlygostev
authors.forename: N A
authors.initials: NA
authors.lastname: Mikhaĭlova
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000096:biosynthesis* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity*; D005285:Fermentation; D005818:Genetic Engineering; D051379:Mice; D011552:Pseudomonas Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D054406:Pseudomonas Vaccines / Q000008:administration & dosage / Q000096:biosynthesis* / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000096:biosynthesis* / Q000235:genetics; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D054406:Pseudomonas Vaccines; D011994:Recombinant Proteins