title: On the preparation of indoxyl red from indican and some new characteristics.
abstract: An indole compound with a strong purple-red color was produced by boiling a solution of indican under acidic conditions and purified by chromatographies on DEAE-650S Toyopearl TSK-gel and silica-gel columns. The purple-red compound purified was identified as indoxyl red, on the basis of FAB Mass, (13)C NMR, (1)H NMR, UV-visible spectra, and IR spectra. Although indoxyl red was first synthesized by Seidel(9) 70 years ago, very little information has been available on its characteristics. We repot here that the compound was purple-red colored at acidic pH and green at pH 13, and showed antiproliferative and cytotoxic activities to the mouse B cell lymphoma cell line NSF202.
authors.affiliation: College of Life Sciences, Ritsumeikan University, 1-1-1 Noji-higashi, Kusatsu, Shiga 525-8577, Japan.
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Song
authors.forename: Mizuki
authors.initials: M
authors.lastname: Kitamatsu
authors.forename: Koreyoshi
authors.initials: K
authors.lastname: Imamura
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ohmori
authors.forename: Kouji
authors.initials: K
authors.lastname: Watanabe
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nakanishi
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007200:Indican / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry* / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D051379:Mice; D015394:Molecular Structure
chemical_list: D000970:Antineoplastic Agents; D007211:Indoles; C581838:indoxyl red; C034082:indoxyl; D007200:Indican