title: Assessment of genotoxic potential of two mycotoxins in the wing spot test of Drosophila melanogaster.
abstract: Mycotoxins, the toxic products of molds, exposure causes serious adverse health problems in human, animals, and crops. Determining the potential genotoxic effects of these substances is, therefore, of great importance. We have evaluated the genotoxic toxicity of two trichothecenes--diacetoxyscirpenol (DAS) and T-2 toxin--using the wing somatic mutation and recombination test (SMART) in Drosophila melanogaster. The SMART is based on the principle that the loss of heterozygosis of recessive markers located on the left arm of chromosome 3--multiple wing hairs (mwh) at the map position 0.3 and flare-3 (flr3) at the map position 38.8--may occur through various mechanisms such as mitotic recombination, mutation, deletion, half-translocation, chromosome loss, and nondisjunction. Both the mycotoxins were administered to third instar larvae (72 ± 4 h old) at concentrations ranging from 5 to 40 μM. Based on our results, DAS and T-2 toxins does not exert genotoxic effects up to a concentration of 40 μM.
authors.affiliation: Department of Biology, Faculty of Arts and Sciences, Erzincan University, Erzincan, Turkey mehmet.gurbuzel@hotmail.com.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Gürbüzel
authors.affiliation: Department of Biology, Faculty of Science, Atatürk University, Erzurum, Turkey.
authors.forename: Handan
authors.initials: H
authors.lastname: Uysal
authors.affiliation: Institute of Natural and Applied Sciences, Atatürk University, Erzurum, Turkey.
authors.forename: Halit
authors.initials: H
authors.lastname: Kızılet
mesh_terms: D000818:Animals; D004249:DNA Damage; D004331:Drosophila melanogaster / Q000235:genetics*; D017344:Genes, Insect / Q000187:drug effects*; D007814:Larva / Q000187:drug effects; D009152:Mutagenicity Tests; D009153:Mutagens / Q000737:chemistry / Q000633:toxicity*; D009183:Mycotoxins / Q000737:chemistry / Q000633:toxicity*; D013605:T-2 Toxin / Q000737:chemistry / Q000633:toxicity*; D014255:Trichothecenes / Q000737:chemistry / Q000633:toxicity*; D014921:Wings, Animal / Q000187:drug effects*
chemical_list: D009153:Mutagens; D009183:Mycotoxins; D014255:Trichothecenes; D013605:T-2 Toxin; C001809:diacetoxyscirpenol
keywords: Drosophila melanogaster; T-2 toxin; diacetoxyscirpenol; genotoxicity; mycotoxin; wing spot test