title: [Vibrio cholerae strains certification].
abstract: OBJECTIVE
  Certification of V. cholerae strains stored at State collection of pathogenic microorganisms and cell cultures SCPM - Obolensk.
  
  
  METHODS
  50 V. cholerae strains were studied. Real-time PCR with primers to genes ctxA, ctxB, ace, zot, tcpA, toxR, hlyA, rtxC, rfbO1, ompU, ompW was used.
  
  
  RESULTS
  Membership of the studied strains in V. cholerae species was confirmed by molecular-biological methods. 46 strains belong to O1 serogroup, 42 of those - E1 Tor toxigenic, having all the virulence genes and 4 non-toxigenic strains. A strain had ace, zot, tcpA, toxR, rtxC, hlyA, ompU genes. 2 strains had ace, toxR, rtxC, hlyA genes, a strain had only toxR gene which is a global regulatory gene. 2 of the 4 serogroup O1 strains were non-toxigenic and had all the virulence genes (ctxA, ctxB, ace, zot, tcpA, toxR, rtxC, hlyA, ompU). 1 non-toxigenic strain has ace, zot, toxR, hlyA, ompUgenes, and the other - toxR, hlyAgenes.
  
  
  CONCLUSIONS
  Genome certificates of all the V. cholerae strains stored in State collection of pathogenic microorganisms and cell cultures SCPM - Obolensk were created. Markers of epidemic threat - ctxA, ctxB, tcpA, toxR and additional virulence genes were determined.
authors.forename: A A
authors.initials: AA
authors.lastname: Kislichkina
authors.forename: V N
authors.initials: VN
authors.lastname: Stepanshina
authors.forename: A V
authors.initials: AV
authors.lastname: Nizova
authors.forename: N V
authors.initials: NV
authors.lastname: Maĭskaia
authors.forename: T N
authors.initials: TN
authors.lastname: Mukhina
authors.forename: R I
authors.initials: RI
authors.lastname: Mironova
authors.forename: I G
authors.initials: IG
authors.lastname: Shemiakin
mesh_terms: D002772:Cholera Toxin / Q000235:genetics; D017931:DNA Primers; D036781:Fimbriae Proteins / Q000235:genetics*; D005809:Genes, Regulator; D044404:Genomic Islands / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics*
chemical_list: D017931:DNA Primers; D014157:Transcription Factors; D036781:Fimbriae Proteins; D002772:Cholera Toxin