title: Detection of Fungi in Indoor Environments and Fungus-Specific IgE Sensitization in Allergic Children.
abstract: BACKGROUND
  : The aim of this study is to investigate relationships between fungal colonization in the house and IgE sensitization to fungi, and to clarify the effects of house care in relation to fungi.
  
  
  METHODS
  : We measured levels of fungi in the houses of 52 allergic children. Of these, 32 children displayed detectable levels of IgE (≥0.35 UA/ml) to a combination of fungi (positive group). The remaining 20 children were not sensitized to fungi (negative group). Each fungus-specific IgE level was also measured in sera of the positive group, and a questionnaire-based survey was conducted for daily lifestyles.
  
  
  RESULTS
  : Cladosporium was the most prevalent in the houses. From the 32 sera of the positive group, specific IgE levels ≥0.70 UA/ml were most frequently detected in 21 sera for Alternaria. Children in whose houses Alternaria was found displayed higher levels of Alternaria-IgE than those in whose houses where Alternaria was not found. In addition, Alternaria-IgE level was lower for children using an air purifier than for children who were not. Windows were more frequently opened for ventilation in negative-group houses than in positive-group houses.
  
  
  CONCLUSIONS
  : The existence of Alternaria might strongly induce IgE sensitization for Alternaria. Using an air purifier and frequently opening windows may minimize fungal sensitization of allergic children.
authors.affiliation: From the 1Department of Pediatrics and 2Department of Clinical Pathology, Tenri Hospital, Tenri, Nara, Japan; the 3Division of Microbiology, National Institute of Health Sciences, Tokyo, Japan; and 4Institute of Tokyo Environmental Allergy, Tokyo, Japan.
authors.forename: Mitsuhiko
authors.initials: M
authors.lastname: Nambu
authors.forename: Hisashi
authors.initials: H
authors.lastname: Kouno
authors.forename: Maki
authors.initials: M
authors.lastname: Aihara-Tanaka
authors.forename: Hideharu
authors.initials: H
authors.lastname: Shirai
authors.forename: Kosuke
authors.initials: K
authors.lastname: Takatori