title: Susceptibility of four different honey bee species to Nosema ceranae.
abstract: In this study, we investigated the infectivity of Nosema ceranae and the immune response of the European honey bee, Apis mellifera and the Asian honey bee species, Apis cerana, Apis dorsata and Apis florea when inoculated with two isolates of N. ceranae isolated from different climates (Canada and Thailand), using cage experiments. The results indicated that the local isolate of N. ceranae (Thailand) had high infectivity in A. mellifera, A. cerana and A. dorsata but only a few spores were observed in A. florea. However, we found that only two honey bee species, A. mellifera and A. dorsata became infected when inoculated with N. ceranae isolated from Canada. Finally, our results showed that transcript levels of antimicrobial peptides (AMPs) in Asian honey bees were significantly higher than that of A. mellifera in both the control and N. ceranae inoculated bee groups. Comparing the expression of AMPs between the control and inoculated bees in each species, it was evident that N. ceranae inoculations did not affect the expression level of abaecin in all four honey bees species investigated in this experiment. Nevertheless, we found a significant up-regulation of apidaecin in A. cerana and A. florea when inoculated with N. ceranae (Canadian isolate). Also, the mRNA levels of hymenoptaecin were significantly increased in A. cerana after inoculation by N. ceranae isolated from Canada as compared with the Thai isolate.
authors.affiliation: Bee Protection Center, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Veeranan
authors.initials: V
authors.lastname: Chaimanee
authors.forename: Jeffery S
authors.initials: JS
authors.lastname: Pettis
authors.forename: Yanping
authors.initials: Y
authors.lastname: Chen
authors.forename: Jay D
authors.initials: JD
authors.lastname: Evans
authors.forename: Kitiphong
authors.initials: K
authors.lastname: Khongphinitbunjong
authors.forename: Panuwan
authors.initials: P
authors.lastname: Chantawannakul
mesh_terms: D000818:Animals; D001516:Bees / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*; D018076:DNA, Complementary; D005786:Gene Expression Regulation / Q000276:immunology; D054884:Host-Pathogen Interactions / Q000276:immunology; D016818:Nosema / Q000276:immunology / Q000502:physiology*; D012313:RNA / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013172:Spores, Fungal
chemical_list: D018076:DNA, Complementary; D012313:RNA