title: De novo assembly and functional annotation of the olive (Olea europaea) transcriptome.
abstract: Olive breeding programmes are focused on selecting for traits as short juvenile period, plant architecture suited for mechanical harvest, or oil characteristics, including fatty acid composition, phenolic, and volatile compounds to suit new markets. Understanding the molecular basis of these characteristics and improving the efficiency of such breeding programmes require the development of genomic information and tools. However, despite its economic relevance, genomic information on olive or closely related species is still scarce. We have applied Sanger and 454 pyrosequencing technologies to generate close to 2 million reads from 12 cDNA libraries obtained from the Picual, Arbequina, and Lechin de Sevilla cultivars and seedlings from a segregating progeny of a Picual × Arbequina cross. The libraries include fruit mesocarp and seeds at three relevant developmental stages, young stems and leaves, active juvenile and adult buds as well as dormant buds, and juvenile and adult roots. The reads were assembled by library or tissue and then assembled together into 81 020 unigenes with an average size of 496 bases. Here, we report their assembly and their functional annotation.
authors.affiliation: Department of Integrated Bioinformatics, National Institute for Bioinformatics, University of Málaga, Campus de Teatinos, Málaga, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Muñoz-Mérida
authors.forename: Juan José
authors.initials: JJ
authors.lastname: González-Plaza
authors.forename: Andrés
authors.initials: A
authors.lastname: Cañada
authors.forename: Ana María
authors.initials: AM
authors.lastname: Blanco
authors.forename: Maria del Carmen
authors.initials: Mdel C
authors.lastname: García-López
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Rodríguez
authors.forename: Laia
authors.initials: L
authors.lastname: Pedrola
authors.forename: M Dolores
authors.initials: MD
authors.lastname: Sicardo
authors.forename: M Luisa
authors.initials: ML
authors.lastname: Hernández
authors.forename: Raúl
authors.initials: R
authors.lastname: De la Rosa
authors.forename: Angjelina
authors.initials: A
authors.lastname: Belaj
authors.forename: Mayte
authors.initials: M
authors.lastname: Gil-Borja
authors.forename: Francisco
authors.initials: F
authors.lastname: Luque
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Martínez-Rivas
authors.forename: David G
authors.initials: DG
authors.lastname: Pisano
authors.forename: Oswaldo
authors.initials: O
authors.lastname: Trelles
authors.forename: Victoriano
authors.initials: V
authors.lastname: Valpuesta
authors.forename: Carmen R
authors.initials: CR
authors.lastname: Beuzón
mesh_terms: D001947:Breeding; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D005638:Fruit / Q000737:chemistry; D015723:Gene Library; D018745:Genome, Plant; D058977:Molecular Sequence Annotation; D031658:Olea / Q000235:genetics*; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry; D012639:Seeds / Q000235:genetics; D017422:Sequence Analysis, DNA; D059467:Transcriptome
chemical_list: D000069463:Olive Oil; D010938:Plant Oils