title: Antipyrine based arsenate selective fluorescent probe for living cell imaging.
abstract: Condensation of salicylaldehyde and 4-aminoantipyrine has yielded a new fluorescent probe (APSAL) capable of detecting intracellular arsenate at the micromolar level for the first time. The structure of the probe has been established by different spectroscopic techniques and confirmed from X-ray crystallography. Common anions, viz., F(-), Cl(-), Br(-), I(-), N(3)(-), NCO(-), NO(2)(-), NO(3)(-), SCN(-), CN(-), CH(3)COO(-), SO(4)(2-), ClO(4)(-), and HPO(4)(2-) do not interfere. The binding constant of APSAL for H(2)AsO(4)(-) has been determined using the Benesi-Hildebrand equation as 8.9 × 10(3) M(-1). Fluorescence quantum yield of APSAL (0.016) increases more than 12 times upon binding arsenate ion.
authors.affiliation: Department of Chemistry, The University of Burdwan, Burdwan, West Bengal, India.
authors.forename: Sisir
authors.initials: S
authors.lastname: Lohar
authors.forename: Animesh
authors.initials: A
authors.lastname: Sahana
authors.forename: Arnab
authors.initials: A
authors.lastname: Banerjee
authors.forename: Avishek
authors.initials: A
authors.lastname: Banik
authors.forename: Subhra Kanti
authors.initials: SK
authors.lastname: Mukhopadhyay
authors.forename: Jesús Sanmartín
authors.initials: JS
authors.lastname: Matalobos
authors.forename: Debasis
authors.initials: D
authors.lastname: Das
mesh_terms: D000983:Antipyrine / Q000737:chemistry*; D001149:Arsenates / Q000737:chemistry*; D002462:Cell Membrane / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D057054:Molecular Imaging / Q000379:methods*; D014961:X-Ray Diffraction
chemical_list: D001149:Arsenates; D005456:Fluorescent Dyes; C025657:arsenic acid; D000983:Antipyrine