title: Müller glia cells activation in rat retina after optic nerve injury: spatiotemporal correlation with transcription initiation factor IIb.
abstract: Transcription initiation factor IIB (TFIIB) is an ideal factor to localize core promoters and plays a central role in the assembly of the pre-initiation complex. Previous studies showed that the assembly of TFIIB played an important role in rat ischemic brain injury. To elucidate the expression and possible functions of TFIIB in retina lesion and repair, we performed an optic nerve crush (ONC) model in adult rats. Western blot analysis and immunohistochemistry showed a significant upregulation of TFIIB in retina after ONC. Immunofluorescent labeling indicated that TFIIB was localized mainly in the Müller glia cells (MGCs); colocalization of TFIIB and proliferating cell nuclear antigen (PCNA) in the injured retina suggested that TFIIB might participate in MGCs proliferation. In addition, we also examined the expression of the retinal progenitor markers (Nestin and Pax6) whose changes were correlated with the expression of TFIIB. In vitro, we induced MGCs differentiation with brain nerve growth factor (BNGF) and found that TFIIB expression was increased in the differentiated process, which was collected with the expression of PCNA, Nestin, and Pax6. Additionally, knocking TFIIB down with siRNA inhibited the expression of PCNA, Nestin, and Pax6. Collectively, we hypothesized ONC-induced upregulation of TFIIB in the retina was associated with MGCs activation and differentiation.
authors.affiliation: Department of Ophthalmology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Xu
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Nan
authors.initials: N
authors.lastname: Jin
authors.forename: Juming
authors.initials: J
authors.lastname: Zhu
authors.forename: Lihua
authors.initials: L
authors.lastname: Kang
authors.forename: Tianqiu
authors.initials: T
authors.lastname: Zhou
authors.forename: Junjun
authors.initials: J
authors.lastname: Wang
authors.forename: Aiqin
authors.initials: A
authors.lastname: Sheng
authors.forename: Jian
authors.initials: J
authors.lastname: Shi
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Gu
authors.forename: Aimin
authors.initials: A
authors.lastname: Sang
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D063928:Ependymoglial Cells / Q000166:cytology / Q000378:metabolism*; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D009409:Nerve Crush; D064231:Nestin / Q000235:genetics / Q000378:metabolism; D020221:Optic Nerve Injuries / Q000378:metabolism*; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors / Q000235:genetics / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D035581:Transcription Factor TFIIB / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D005136:Eye Proteins; D018398:Homeodomain Proteins; C577486:Nes protein, rat; D064231:Nestin; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; C000605733:Pax6 protein, rat; D018809:Proliferating Cell Nuclear Antigen; D012097:Repressor Proteins; D035581:Transcription Factor TFIIB