title: Catalysis of a new ribose carbon-insertion reaction by the molybdenum cofactor biosynthetic enzyme MoaA.
abstract: MoaA, a radical S-adenosylmethionine enzyme, catalyzes the first step in molybdopterin biosynthesis. This reaction involves a complex rearrangement in which C8 of guanosine triphosphate is inserted between C2' and C3' of the ribose. This study identifies the site of initial hydrogen atom abstraction by the adenosyl radical and advances a mechanistic proposal for this unprecedented reaction.
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, TX 77842, USA.
authors.forename: Angad P
authors.initials: AP
authors.lastname: Mehta
authors.forename: Jeremiah W
authors.initials: JW
authors.lastname: Hanes
authors.forename: Sameh H
authors.initials: SH
authors.lastname: Abdelwahed
authors.forename: David G
authors.initials: DG
authors.lastname: Hilmey
authors.forename: Petra
authors.initials: P
authors.lastname: Hänzelmann
authors.forename: Tadhg P
authors.initials: TP
authors.lastname: Begley
mesh_terms: D002244:Carbon; D002384:Catalysis; D003067:Coenzymes / Q000737:chemistry / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D006867:Hydrolases / Q000737:chemistry* / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008667:Metalloproteins / Q000737:chemistry / Q000378:metabolism*; D008956:Models, Chemical; D000090006:Molybdenum Cofactors; D011621:Pteridines / Q000737:chemistry / Q000378:metabolism*; D012266:Ribose / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D002244:Carbon; D003067:Coenzymes; D006160:Guanosine Triphosphate; D006867:Hydrolases; D008667:Metalloproteins; D000090006:Molybdenum Cofactors; D011621:Pteridines; D012266:Ribose; C022261:molybdopterin; C511316:S-adenosylmethionine enzyme MoaA, Staphylococcus aureus