title: Quantitative detection of Aspergillus spp. by real-time nucleic acid sequence-based amplification.
abstract: Rapid and quantitative detection of Aspergillus from clinical samples may facilitate an early diagnosis of invasive pulmonary aspergillosis (IPA). As nucleic acid-based detection is a viable option, we demonstrate that Aspergillus burdens can be rapidly and accurately detected by a novel real-time nucleic acid assay other than qPCR by using the combination of nucleic acid sequence-based amplification (NASBA) and the molecular beacon (MB) technology. Here, we detail a real-time NASBA assay to determine quantitative Aspergillus burdens in lungs and bronchoalveolar lavage (BAL) fluids of rats with experimental IPA.
authors.affiliation: Public Health Research Institute Center, UMDNJ-NJMS, Newark, NJ, USA.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Zhao
authors.forename: David S
authors.initials: DS
authors.lastname: Perlin
mesh_terms: D000818:Animals; D001230:Aspergillus / Q000235:genetics* / Q000302:isolation & purification*; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D002138:Calibration; D055744:Invasive Pulmonary Aspergillosis / Q000382:microbiology; D008168:Lung / Q000382:microbiology; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009691:Nucleic Acid Denaturation; D015345:Oligonucleotide Probes / Q000737:chemistry; D051381:Rats; D013997:Time Factors; D044366:Transition Temperature
chemical_list: D015345:Oligonucleotide Probes