title: [Mycoplasma pneumoniae capsular polysaccharides bind to DC-SIGN and promote the secretion of IL-10].
abstract: OBJECTIVE
  To explore the interaction of Mycoplasma pneumoniae (M. pneumoniae) capsular polysaccharide (CPS) with dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN) and its influence on the secretions of IL-10 and IL-12.
  
  
  METHODS
  M. pneumoniae CPS and lipid-associated membrane proteins (LAMPs) were extracted and purified, and the specific binding of CPS and DC-SIGN was detected using indirect immunofluorescence staining. The expressions of IL-10 and IL-12 was tested by ELISA in immature DC after treated by CPS or/and LAMPs.
  
  
  RESULTS
  Indirect immunofluorescence staining showed CPS could recognized and bind specifically to the DC, which could be blocked by the DC-SIGN-specific blocking antibody. ELISA revealed that the production of immunosuppressive cytokine IL-10 increased when immature DC were stimulated by CPS, and the effect was more obvious with the presence of LAMPs(P<0.05). But the production of IL-12 did not change significantly after stimulation of CPS or/and LAMPs.
  
  
  CONCLUSIONS
  The CPS of M.pneumoniae can recognize and bind to the DC-SIGN specifically and promote the immature DC to secret IL-10.
authors.affiliation: Department of Microbiology and Immunology, University of South China, Hengyang 421001, China. moonchatstone@yahoo.com
authors.forename: Ziling
authors.initials: Z
authors.lastname: Liu
authors.forename: Xiaoxing
authors.initials: X
authors.lastname: You
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Peng
authors.forename: Huili
authors.initials: H
authors.lastname: Zhang
authors.forename: Shunli
authors.initials: S
authors.lastname: Gao
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Zeng
authors.forename: Minjun
authors.initials: M
authors.lastname: Yu
authors.forename: Cuiming
authors.initials: C
authors.lastname: Zhu
mesh_terms: D015815:Cell Adhesion Molecules / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism*; D018664:Interleukin-12 / Q000378:metabolism; D037181:Lectins, C-Type / Q000276:immunology / Q000378:metabolism*; D009177:Mycoplasma pneumoniae / Q000276:immunology*; D011019:Pneumonia, Mycoplasma / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology / Q000378:metabolism*; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000276:immunology / Q000378:metabolism*; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015815:Cell Adhesion Molecules; D016753:Interleukin-10; D018664:Interleukin-12; D037181:Lectins, C-Type; D011135:Polysaccharides, Bacterial; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin