title: Improvement of free fatty acid production in Escherichia coli using codon-optimized Streptococcus pyogenes acyl-ACP thioesterase.
abstract: Fatty acyl-acyl carrier protein (ACP) thioesterase (acyl-ACP TE) from Streptococcus pyogenes (strain MGAS10270) was codon-optimized and expressed in Escherichia coli K-12 W3110 and Escherichia coli K-12 MG1655. By employing codon-optimized S. pyogenes acyl-ACP TE to improve the total free fatty acids (FFAs) and to tailor the composition of FFAs, high-specificity production of saturated fatty acids (C12, C14) and unsaturated fatty acids (C18:1 C18:2) was achieved in recombinants. E. coli SGJS41 and SGJS46 (codon-optimized acyl-ACP TE of S. pyogenes) demonstrated the highest intracellular total FFA content (339 mg/l vs 342 mg/l); in particular, the content of C12 and C14 FFAs was about 3-5 fold, and the content of C18:1 and C18:2 FFAs was about 8-42 fold higher than that in the control E. coli and E. coli JES1017 (original acyl-ACP TE of S. pyogenes).
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 121-742, Republic of Korea.
authors.forename: Sunhee
authors.initials: S
authors.lastname: Lee
authors.forename: Soohyun
authors.initials: S
authors.lastname: Park
authors.forename: Jinwon
authors.initials: J
authors.lastname: Lee
mesh_terms: D001483:Base Sequence; D003062:Codon; D003470:Culture Media; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000096:biosynthesis*; D008401:Gas Chromatography-Mass Spectrometry; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics; D013297:Streptococcus pyogenes / Q000201:enzymology*; D013869:Thiolester Hydrolases / Q000235:genetics*
chemical_list: D003062:Codon; D003470:Culture Media; D004274:DNA, Recombinant; D005230:Fatty Acids, Nonesterified; D011994:Recombinant Proteins; D013869:Thiolester Hydrolases; C018748:oleoyl-(acyl-carrier-protein) hydrolase