title: Investigation of gold nanoparticles uptake and their tissue level distribution in rice plants by laser ablation-inductively coupled-mass spectrometry.
abstract: The tissue level uptake and spatial distribution of gold nanoparticles (AuNPs) in rice (Oryza sativa L.) roots and shoots under hydroponic conditions was investigated using laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). Rice plants were hydroponically exposed to positively, neutrally, and negatively charged AuNPs [AuNP1(+), AuNP2(0), AuNP3(-)] with a core diameter of 2 nm. Plants were exposed to AuNPs having 1.6 mg Au/L for 5 days or 0.14 mg Au/L for 3 months to elucidate how the surface charges of the nanoparticles affects their uptake into living plant tissues. The results demonstrate that terminal functional groups greatly affected the AuNP uptake into plant tissues. Au concentration determined by LA-ICP-MS in 5 day treated rice roots followed this order: AuNP1(+) > AuNP2(0) > AuNP3(-) but this order was reversed for rice shoots, indicating preferential translocation of AuNP3(-). Bioimages revealed distributions of mesophyll and vascular AuNP dependent on organ or AuNP concentration.
authors.affiliation: School of Natural Science, Hampshire College, Amherst, MA 01002, USA.
authors.forename: Jeremy
authors.initials: J
authors.lastname: Koelmel
authors.forename: Thomas
authors.initials: T
authors.lastname: Leland
authors.forename: Huanhua
authors.initials: H
authors.lastname: Wang
authors.forename: Dulasiri
authors.initials: D
authors.lastname: Amarasiriwardena
authors.forename: Baoshan
authors.initials: B
authors.lastname: Xing
mesh_terms: D006046:Gold / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D053685:Laser Therapy; D053768:Metal Nanoparticles / Q000032:analysis / Q000737:chemistry*; D008956:Models, Chemical; D012275:Oryza / Q000737:chemistry* / Q000378:metabolism; D018570:Risk Assessment; D012989:Soil Pollutants / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D013054:Spectrophotometry, Atomic
chemical_list: D012989:Soil Pollutants; D006046:Gold