title: Direct regulation of caspase‑3 by the transcription factor AP‑2α is             involved in aspirin‑induced apoptosis in MDA‑MB‑453 breast cancer cells.
abstract: Aspirin has been reported to trigger apoptosis in various cancer cell lines.             However, the detailed mechanisms involved remain elusive. The present study aimed             to investigate whether aspirin plays a role in apoptosis of MDA-MB-453 cells.             The effect of aspirin on the proliferation of human MDA-MB-453 cells breast cancer             cells was evaluated using MTT assay, flow cytometry and western blotting. The             present study reports that aspirin induces the apoptosis of MDA‑MB‑453 breast             cancer cells which was attributed to the increased expression and activation of             caspase‑3. Moreover, AP‑2α, a transcription factor highly expressed in MDA‑MB‑453             cells, was identified as a negative regulator of caspase‑3 transcription and AP‑2α             was attenuated following aspirin treatment. Therefore, aspirin may increase the             expression of caspase‑3 by inducing the degradation of AP‑2α, which increases             activated caspase‑3 expression, thereby triggering apoptosis in MDA‑MB‑453 cells.             Thus, aspirin may be used in breast cancer therapy.
authors.affiliation: Key Laboratory of Protein Chemistry and Development Biology of State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yan
authors.forename: Qiongzhi
authors.initials: Q
authors.lastname: He
authors.forename: Xiang
authors.initials: X
authors.lastname: Hu
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.forename: Ke
authors.initials: K
authors.lastname: Wei
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Yingli
authors.initials: Y
authors.lastname: Zhong
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ding
authors.forename: Shuanglin
authors.initials: S
authors.lastname: Xiang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects*; D001241:Aspirin / Q000633:toxicity*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D011401:Promoter Regions, Genetic; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D011485:Protein Binding; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D050656:Transcription Factor AP-2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D034741:RNA, Small Interfering; D050656:Transcription Factor AP-2; D053148:Caspase 3; D046988:Proteasome Endopeptidase Complex; D001241:Aspirin