title: The role of HSPA12B in regulating neuronal apoptosis.
abstract: Heat shock protein A12B (HSPA12B) is the newest member of a recently defined subfamily of proteins distantly related to the 70-kDa family of heat shock proteins (HSP70) family. HSP70s play a crucial role in protecting cells, tissues, organs and animals from various noxious conditions. Here we studied the dynamic expression changes and localization of HSPA12B after middle cerebral artery occlusion (MCAO) with reperfusion induced ischemic insult processes in adult rats. Apoptosis, as indicated by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, was also increased in the peri-ischemic cortex compared to non-ischemic hemisphere. The expression of HSPA12B was strongly induced in the ischemic hemisphere of MCAO reperfusion rats in vivo. In vitro studies indicated that the up-regulation of HSPA12B may be involved in oxygen-glucose deprivation-induced PC12 cell death. And knockdown of HSPA12B in cultured differentiated PC12 cells by siRNA showed that HSPA12B inhibited the expression of active caspase-3. Collectively, these results suggested that HSPA12B may be required for protecting neurons from ischemic insults.
authors.affiliation: The Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, Jiangsu, People's Republic of China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Kang
authors.forename: Guowei
authors.initials: G
authors.lastname: Zhang
authors.forename: Yaohua
authors.initials: Y
authors.lastname: Yan
authors.forename: Kaifu
authors.initials: K
authors.lastname: Ke
authors.forename: Xinmin
authors.initials: X
authors.lastname: Wu
authors.forename: Yilu
authors.initials: Y
authors.lastname: Gao
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Zhu
authors.forename: Qiyun
authors.initials: Q
authors.lastname: Wu
authors.forename: Zhengming
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000502:physiology*; D020244:Infarction, Middle Cerebral Artery / Q000378:metabolism / Q000473:pathology; D008297:Male; D009474:Neurons / Q000166:cytology / Q000473:pathology / Q000502:physiology*; D016716:PC12 Cells; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018840:HSP70 Heat-Shock Proteins; C552943:HSPA12B protein, rat