title: Study on the binding of chloroamphenicol with bovine serum albumin by fluorescence and UV-vis spectroscopy.
abstract: The binding of chloroamphenicol (CPC) to bovine serum albumin (BSA) at 296 K, 303 K, and 310 K by fluorescence and UV-visible absorption spectroscopy were investigated under imitated physiological conditions. The experimental results showed that the fluorescence quenching mechanism between CPC and BSA was combined quenching (dynamic and static quenching) procedure at low CPC concentration, or a dynamic quenching procedure at high concentrations. The binding constants, binding sites and the corresponding thermodynamic parameters of the interaction system were calculated. According to Förster non-radiation energy transfer theory, the binding distance between CPC and BSA was calculated to be 3.02 nm. Both synchronous fluorescence and FT-IR spectra confirmed the interaction, and indicated the conformational changes of BSA. The effects of some common metal ions Ca(2+), Ni(2+), Mg(2+), Fe(2+), and Cu(2+) on the binding constant between CPC and BSA were examined. Furthermore, we investigated the possible sub-domains on BSA that bind CPC by displacement experiments.
authors.affiliation: Department of Physics, School of Physics and Information Engineering, Fuzhou University, Fuzhou 350108, China. zhangjunfz@163.com
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Lingnan
authors.initials: L
authors.lastname: Chen
authors.forename: Birong
authors.initials: B
authors.lastname: Zeng
authors.forename: Qilong
authors.initials: Q
authors.lastname: Kang
authors.forename: Lizong
authors.initials: L
authors.lastname: Dai
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D002417:Cattle; D002701:Chloramphenicol / Q000737:chemistry / Q000378:metabolism*; D007477:Ions / Q000378:metabolism; D008670:Metals / Q000378:metabolism; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000900:Anti-Bacterial Agents; D007477:Ions; D008670:Metals; D012710:Serum Albumin, Bovine; D002701:Chloramphenicol