title: Development of a stable insect cell line constitutively expressing rotavirus VP2.
abstract: An insect High-Five cell line was generated constitutively and stably expressing the VP2 protein of rotavirus RF strain leading to the formation of core-like particles. The integration of the VP2 gene was confirmed by PCR, Real-time PCR and Southern blot, and the protein expression was confirmed by Western blot and immunofluorescence microscopy. Integrity and self assembly of VP2 as core-like particles was demonstrated by electron microscopy. The High-Five cell system stably expressing rotavirus core-like particles can be applied to the study of viral protein structure and functions; it may be useful for vaccine development, gene therapy and drug delivery.
authors.affiliation: School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Zabihollah
authors.initials: Z
authors.lastname: Shoja
authors.forename: Maria
authors.initials: M
authors.lastname: Tagliamonte
authors.forename: Somayeh
authors.initials: S
authors.lastname: Jalilvand
authors.forename: Talat
authors.initials: T
authors.lastname: Mokhtari-Azad
authors.forename: Rasool
authors.initials: R
authors.lastname: Hamkar
authors.forename: Shohreh
authors.initials: S
authors.lastname: Shahmahmoodi
authors.forename: Farhad
authors.initials: F
authors.lastname: Rezaei
authors.forename: MariaLina
authors.initials: M
authors.lastname: Tornesello
authors.forename: Franco M
authors.initials: FM
authors.lastname: Buonaguro
authors.forename: Luigi
authors.initials: L
authors.lastname: Buonaguro
authors.forename: Rakhshandeh
authors.initials: R
authors.lastname: Nategh
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D015153:Blotting, Western; D036022:Capsid Proteins / Q000096:biosynthesis*; D002460:Cell Line; D007313:Insecta; D046911:Macromolecular Substances / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D055503:Protein Multimerization; D060888:Real-Time Polymerase Chain Reaction; D022701:Virosomes / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D036022:Capsid Proteins; D046911:Macromolecular Substances; C442545:VP2 protein, Rotavirus; D022701:Virosomes