title: Gene expression profiling and histomorphometric analyses of the early bone healing response around nanotextured implants.
abstract: While in vitro studies have shown that nanoscale surface modifications influence cell fate and activity, there is little information on how they modulate healing at the bone-implant interface.
  
  
  OBJECTIVE
  This study aims to investigate the effect of nanotopography at early time intervals when critical events for implant integration occur.
  
  
  METHODS
  Untreated and sulfuric acid/hydrogen peroxide-treated machined-surface titanium alloy implants were placed in rat tibiae. Samples were processed for DNA microarray analysis and histomorphometry.
  
  
  RESULTS
  At both 3 and 5 days, the gene expression profile of the healing tissue around nanotextured implants differed from that around machined-surface implants or control empty holes, and were accompanied by an increase in bone-implant contact on day 5. While some standard pathways such as the immune response predominated, a number of unclassified genes were also implicated.
  
  
  CONCLUSIONS
  Nanotexture elicits an initial gene response that is more complex than suspected so far and favors healing at the bone-implant interface.
authors.affiliation: Laboratory for the Study of Calcified Tissues & Biomaterials, Department of Stomatology, Université de Montréal, PO Box 6128, Station Centre-Ville, Montreal, QC, H3C 3J7, Canada.
authors.forename: Rima M
authors.initials: RM
authors.lastname: Wazen
authors.forename: Shingo
authors.initials: S
authors.lastname: Kuroda
authors.forename: Clarice
authors.initials: C
authors.lastname: Nishio
authors.forename: Karine
authors.initials: K
authors.lastname: Sellin
authors.forename: John B
authors.initials: JB
authors.lastname: Brunski
authors.forename: Antonio
authors.initials: A
authors.lastname: Nanci
mesh_terms: D000818:Animals; D001846:Bone Development / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D049329:Nanostructures / Q000009:adverse effects / Q000627:therapeutic use*; D020411:Oligonucleotide Array Sequence Analysis; D010012:Osteogenesis / Q000235:genetics*; D019736:Prostheses and Implants; D051381:Rats; D013977:Tibia / Q000601:surgery; D014025:Titanium / Q000627:therapeutic use; D014945:Wound Healing / Q000235:genetics
chemical_list: D014025:Titanium