title: Beneficial effects of aminoguanidine on skin flap survival in diabetic rats.
abstract: Random flaps in DM patients have poor reliability for wound coverage, and flap loss remains a complex challenge. The protective effects of aminoguanidine (AG) administration on the survival of dorsal random flaps and oxidative stress were studied in diabetic rats. Two months after the onset of DM, dorsal McFarlane flaps were raised. Forty rats were divided into four groups: (1) control, (2) AG, (3) DM, and (4) DM + AG groups. Flap viability, determined with the planimetric method, and free-radical measurements were investigated. In addition, HbA1c and blood glucose levels, body weight measurements, and histopathological examinations were evaluated. The mean flap necrotic areas (%) in Groups I to IV were 50.9 ± 13.0, 32.9 ± 12.5, 65.2 ± 11.5, and 43.5 ± 14.7, respectively. The malondialdehyde (MDA) and nitric oxide (NO) levels were higher in the DM group than in the nondiabetic group, while the reduced glutathione (GSH) levels and superoxide dismutase (SOD) activity were reduced as a result of flap injury. In the diabetic and nondiabetic groups, AG administration significantly reduced the MDA and NO levels and significantly increased GSH content and SOD enzyme activity. We concluded that AG plays an important role in preventing random pattern flap necrosis.
authors.affiliation: Department of Plastic Reconstructive and Aesthetic Surgery, Inonu University School of Medicine, 44380 Malatya, Turkey.
authors.forename: Ayse
authors.initials: A
authors.lastname: Ozturk
authors.forename: Cemal
authors.initials: C
authors.lastname: Fırat
authors.forename: Hakan
authors.initials: H
authors.lastname: Parlakpınar
authors.forename: Aysun
authors.initials: A
authors.lastname: Bay-Karabulut
authors.forename: Hale
authors.initials: H
authors.lastname: Kirimlioglu
authors.forename: Ali
authors.initials: A
authors.lastname: Gurlek
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001786:Blood Glucose / Q000378:metabolism; D048909:Diabetes Complications / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000150:complications*; D005260:Female; D005978:Glutathione / Q000378:metabolism; D006442:Glycated Hemoglobin / Q000378:metabolism; D006146:Guanidines / Q000494:pharmacology*; D008315:Malondialdehyde / Q000378:metabolism; D008833:Microcirculation; D009336:Necrosis; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism; D013524:Surgical Flaps / Q000098:blood supply / Q000473:pathology
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D005978:Glutathione; D006442:Glycated Hemoglobin; D006146:Guanidines; D008315:Malondialdehyde; D009569:Nitric Oxide; D013482:Superoxide Dismutase; C004479:pimagedine