title: T cell receptor vβ gene expression in inflammatory bowel disease lamina propria lymphocytes: evidence for altered vβ gene usage.
abstract: : In this study the TCR-Vβ repertoire expressed in T cells of lamina propria mononuclear cells (LPMC) and peripheral blood mononuclear cells (PBMC) was examined using a reverse transcription-PCR (RT-PCR) technique for TCR-Vβ mRNA. Using a qualitative RT-PCR method, LPMC of patients with IBD and control individuals were shown to contain mRNA for each of 20 TCR-Vβ families, indicating that IBD is not associated with a major deletion or expansion of any TCR-Vβ family. Subsequently, using a quantitative method for four frequently expressed TCR-Vβ families, it was shown that the pattern of TCR-Vβ expression was different in PBMC and LPMC of both IBD patients and control individuals. In addition, it was shown that the LPMC/PBMC ratio of mean mRNA values for TCR-Vβ2, but not for TCR-Vβ6, 7, and 14 was lower in IBD patients than control individuals. These results show that the TCR-Vβ repertoire in PBMC and LPMC is different both in IBD patients and control individuals. In addition, they show that the TCR-Vβ repertoire is altered in IBD, possibly due to an immune response to disease specific antigens, superantigens or neoantigens.
authors.affiliation: Mucosal Immunity Section, Laboratory of Clinical Investigation, and *Office of the Director of Intramural Research, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland; †Department of Gastroenterology and Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio; and ‡Division of Gastroenterology, Department of Medicine, University of Maryland at Baltimore, Baltimore, Maryland, U.S.A.
authors.forename: R
authors.initials: R
authors.lastname: Duchmann
authors.forename: W
authors.initials: W
authors.lastname: Strober
authors.forename: D W
authors.initials: DW
authors.lastname: Alling
authors.forename: C
authors.initials: C
authors.lastname: Fiocchi
authors.forename: S P
authors.initials: SP
authors.lastname: James