title: Purification, crystallization and preliminary X-ray crystallographic analysis of diaminopimelate epimerase from Acinetobacter baumannii.
abstract: The meso isomer of diaminopimelate (meso-DAP) is a biosynthetic precursor of L-lysine in bacteria and plants, and is a key component of the peptidoglycan layer in the cell walls of Gram-negative and some Gram-positive bacteria. Diaminopimelate epimerase (DapF) is a pyridoxal-5'-phosphate-independent racemase which catalyses the interconversion of (6S,2S)-2,6-diaminopimelic acid (LL-DAP) and meso-DAP. In this study, DapF from Acinetobacter baumannii was overexpressed in Escherichia coli strain SoluBL21, purified and crystallized using a vapour-diffusion method. A native crystal diffracted to a resolution of 1.9 Å and belonged to space group P3(1) or P3(2), with unit-cell parameters a = b = 74.91, c = 113.35 Å, α = β = 90, γ = 120°. There were two molecules in the asymmetric unit.
authors.affiliation: Division of Magnetic Resonance, Korea Basic Science Institute, 804-1 Yangcheong-ri, Ochang, Chungbuk 363-883, Republic of Korea.
authors.forename: Jeong Soon
authors.initials: JS
authors.lastname: Park
authors.forename: Woo Cheol
authors.initials: WC
authors.lastname: Lee
authors.forename: Jung Hyun
authors.initials: JH
authors.lastname: Song
authors.forename: Seung Il
authors.initials: SI
authors.lastname: Kim
authors.forename: Je Chul
authors.initials: JC
authors.lastname: Lee
authors.forename: Chaejoon
authors.initials: C
authors.lastname: Cheong
authors.forename: Hye Yeon
authors.initials: HY
authors.lastname: Kim
mesh_terms: D040981:Acinetobacter baumannii / Q000201:enzymology*; D000591:Amino Acid Isomerases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics; D011487:Protein Conformation
chemical_list: D001426:Bacterial Proteins; D000591:Amino Acid Isomerases; C042032:diaminopimelate epimerase