title: Single-molecule and bulk approaches to the DnaB replication fork helicase.
abstract: Motor proteins are enzymes that accomplish mechanical work in a wide variety of biological processes. In this review we focus on bulk and single molecule methods to study how motor proteins function. We discuss in detail the analysis of the motor protein DnaB, a hexameric helicase that unwinds DNA at a replication fork in Gram-negative bacteria. Bulk and single-molecule studies have complemented one another to arrive at a comprehensive mechanistic view of how DnaB unwinds double-stranded DNA.
authors.affiliation: Vanderbilt University, Department of Biological Sciences, Nashville, TN 37235, USA. Daniel.Kaplan@Vanderbilt.Edu
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Kaplan
authors.forename: Omar A
authors.initials: OA
authors.lastname: Saleh
authors.forename: Noah
authors.initials: N
authors.lastname: Ribeck
mesh_terms: D004277:DNA, Single-Stranded / Q000378:metabolism*; D053488:DnaB Helicases / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer; D008280:Magnetics / Q000295:instrumentation; D008958:Models, Molecular; D020409:Molecular Motor Proteins / Q000378:metabolism*
chemical_list: D004277:DNA, Single-Stranded; D020409:Molecular Motor Proteins; D053488:DnaB Helicases