title: Formulation of microspheres containing Crataegus monogyna Jacq. extract with free radical scavenging activity.
abstract: Extracts of Crataegus monogyna Jacq. (hawthorn) show an interesting free radical scavenging (FRS) effect, related to their flavonoids content. Unfortunately, their oral administration is affected by their low bioavailability. The aim of this work is to obtain a multiparticulate drug delivery system for hawthorn extracts for oral administration. The extracts from flowering tops (FL) or fruits (FR) of hawthorn were obtained with maceration, using ethanol as an extraction solvent, and their antioxidant activity was evaluated. FL extract showed the highest FRS activity (EC50 3.72 ± 1.21 µg/ml), so it was selected to prepare microparticulate systems by a spray-drying technique, which were characterized by granulometric analysis, scanning electron microscopy-energy dispersive X-ray spectroscopy, confocal fluorescence microscopy and hyperoside content. Antioxidant activity was evaluated before and after gastrointestinal transit in vitro simulation. Results indicate that the microparticulate systems maintained the antioxidant activity of hawthorn also after gastrointestinal transit in vitro simulation, exhibiting properties suitable for oral administration.
authors.affiliation: Dipartimento di Scienze del Farmaco.
authors.forename: Giulia
authors.initials: G
authors.lastname: Lucconi
authors.forename: Theodora
authors.initials: T
authors.lastname: Chlapanidas
authors.forename: Emanuela
authors.initials: E
authors.lastname: Martino
authors.forename: Raffaella
authors.initials: R
authors.lastname: Gaggeri
authors.forename: Sara
authors.initials: S
authors.lastname: Perteghella
authors.forename: Daniela
authors.initials: D
authors.lastname: Rossi
authors.forename: Silvio
authors.initials: S
authors.lastname: Faragò
authors.forename: Daniele
authors.initials: D
authors.lastname: Vigo
authors.forename: Massimo
authors.initials: M
authors.lastname: Faustini
authors.forename: Simona
authors.initials: S
authors.lastname: Collina
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Torre
mesh_terms: D000284:Administration, Oral; D001713:Biphenyl Compounds / Q000737:chemistry; D027825:Crataegus; D016503:Drug Delivery Systems; D016166:Free Radical Scavengers / Q000008:administration & dosage* / Q000494:pharmacology; D008863:Microspheres; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D010936:Plant Extracts; C007145:crataegus extract; C004931:1,1-diphenyl-2-picrylhydrazyl