title: Genetic similarity of Burkholderia cenocepacia from cystic fibrosis patients.
abstract: Burkholderia cenocepacia may cause serious infections in patients with cystic fibrosis, and this microorganism can be highly transmissible. Pulsed-field gel electrophoresis is widely used to study the dynamics of strain spread in cystic fibrosis patients. The aim of this work was to perform pulsed-field gel electrophoresis-based molecular typing of B. cenocepacia isolates to evaluate the epidemiology of this species at our hospital. A total of 28 isolates from 23 cystic fibrosis patients were analyzed. Initially, we compared isolates obtained from the same patient at different periods of time. We then compared the pulsed-field gel electrophoresis profiles of 15 IIIA isolates, and in a third analysis, evaluated the genetic profile of 8 IIIB isolates from different patients. The pulsed-field gel electrophoresis profiles of isolates from the same patient indicated that they are genetically indistinguishable. Analysis of isolates from different patients revealed the presence of multiple clonal groups. These results do not indicate cross-transmission of a unique clone of B. cenocepacia among cystic fibrosis patients, although this has been observed in some patients. Our findings highlight the importance of adequate patient follow-up at cystic fibrosis centers and adherence to management and segregation measures in cystic fibrosis patients colonized with B. cenocepacia.
authors.affiliation: Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Luana
authors.initials: L
authors.lastname: Pretto
authors.forename: Fernanda
authors.initials: F
authors.lastname: de-Paris
authors.forename: Alice Beatriz
authors.initials: AB
authors.lastname: Mombach Pinheiro Machado
authors.forename: Andreza
authors.initials: A
authors.lastname: Francisco Martins
authors.forename: Afonso Luís
authors.initials: AL
authors.lastname: Barth
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods; D019121:Burkholderia Infections / Q000382:microbiology*; D057508:Burkholderia cenocepacia / Q000235:genetics* / Q000302:isolation & purification; D003550:Cystic Fibrosis / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D010641:Phenotype; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial