title: Fabrication and characterization of a thermostable quinoprotein aldose sugar dehydrogenase immobilized electrode.
abstract: We fabricated a thermostable quinoprotein aldose sugar dehydrogenase (tPQQ-ASD derived from a hyperthermophilic archaeon Pyrobaculum aerophilum) immobilized electrode.  The electrode was prepared by immobilizing agarose gel mixed with the enzyme and carbon nanofiber (CNF) on a carbon paste (CP) electrode containing p-benzoquinone (BQ) as an electron mediator.  The electrocatalytic response was clearly observed by the addition of D-glucose at the electrode.  The electrode properties such as pH, temperature dependency and substrate selectivity basically followed the enzyme properties.  The current response against D-glucose increased with measurement temperatures up to 70°C, and response perturbation caused by dissolved oxygen level was not observed at the electrode.  As for the results of long-term stability evaluation, the current response was stable for 30 days when the electrode was stored in HEPES buffer solution (pH 7.0) at 4°C.
authors.affiliation: Department of Chemical Science and Technology, Institute of Science and Technology, The University of Tokushima, Tokushima, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Yamada
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Hayashi
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Sakuraba
authors.forename: Tomoki
authors.initials: T
authors.lastname: Yabutani
authors.forename: Toshio
authors.initials: T
authors.lastname: Takayanagi
mesh_terms: D016227:Benzoquinones / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002021:Buffers; D002244:Carbon / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry; D005948:Glucose Dehydrogenases / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D057139:Nanofibers / Q000737:chemistry; D041543:Pyrobaculum / Q000201:enzymology; D013379:Substrate Specificity; D013499:Surface Properties
chemical_list: D016227:Benzoquinones; D002021:Buffers; D004800:Enzymes, Immobilized; C004532:quinone; D002244:Carbon; D005948:Glucose Dehydrogenases; C033955:glucose dehydrogenase (pyrroloquinoline-quinone); D005947:Glucose