title: A new rat model for investigation of subglottic stenosis.
abstract: CONCLUSIONS
  This study attempted to develop a new rat model of subglottic stenosis (SGS), resulting from subglottic mucosal injury administered by electrocauterization. Despite failure of the posterior SGS model, the anterior SGS model was considered feasible.
  
  
  OBJECTIVE
  To investigate the feasibility of using rats as an animal model for SGS.
  
  
  METHODS
  Thirty-seven female Sprague-Dawley rats were assigned to the control group or to the anterior or posterior subglottic injury group. Electrical cauterization was performed on the anterior or posterior 180° of the subglottic mucosa. Animals were euthanized at 4 weeks after injury. Histologic features of the subglottis were evaluated as regards changes in the subglottic lumen, lamina propria, cartilage, and epithelium after hematoxylin and eosin and Masson trichrome staining.
  
  
  RESULTS
  Survival in the anterior injury group was 80% (13/15) until 4 weeks, and results of histologic evaluation showed an increase in thickness of lamina propria with fibrosis and cartilage damage, resulting in luminal narrowing. A high rate of mortality was observed in the posterior injury group.
authors.affiliation: Department of Otorhinolaryngology-Head & Neck Surgery, Gachon University, Graduate School of Medicine, Gil Medical Center, Incheon, South Korea.
authors.forename: Joo Hyun
authors.initials: JH
authors.lastname: Woo
authors.forename: Gyu Cheol
authors.initials: GC
authors.lastname: Han
authors.forename: Il Gyu
authors.initials: IG
authors.lastname: Kang
authors.forename: Sun Tae
authors.initials: ST
authors.lastname: Kim
authors.forename: Heung Eog
authors.initials: HE
authors.lastname: Cha
authors.forename: Dong Young
authors.initials: DY
authors.lastname: Kim
mesh_terms: D000402:Airway Obstruction / Q000473:pathology / Q000503:physiopathology; D000818:Animals; D004195:Disease Models, Animal; D004564:Electrocoagulation; D005260:Female; D006801:Humans; D006984:Hypertrophy; D007820:Laryngeal Mucosa / Q000293:injuries / Q000473:pathology / Q000503:physiopathology; D007829:Laryngostenosis / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley