title: Assessing organismal aging in the filamentous fungus Podospora anserina.
abstract: Podospora anserina is an extensively studied model organism to unravel the mechanism of organismal aging. This filamentous fungus is short-lived and accessible to experimentation. Aging and lifespan are controlled by genetic and environmental traits and, in this model, have a strong mitochondrial etiology. Here, we describe methods and protocols to manipulate and study the aging process in P. anserina at different levels including biochemistry, cell biology, genetics, and physiology.
authors.affiliation: Faculty of Biosciences, Institute of Molecular Biosciences, Johann Wolfgang Goethe University Frankfurt, Frankfurt, Germany. osiewacz@bio.uni-frankfurt.de
authors.forename: Heinz D
authors.initials: HD
authors.lastname: Osiewacz
authors.forename: Andrea
authors.initials: A
authors.lastname: Hamann
authors.forename: Sandra
authors.initials: S
authors.lastname: Zintel
mesh_terms: D002374:Catalase / Q000378:metabolism; D005298:Fertility; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism; D050296:Microbial Viability; D008928:Mitochondria / Q000378:metabolism; D025282:Mycelium / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D010100:Oxygen / Q000378:metabolism; D010641:Phenotype; D048468:Podospora / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D012331:RNA, Fungal / Q000302:isolation & purification / Q000378:metabolism; D013172:Spores, Fungal / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D013312:Stress, Physiological; D013997:Time Factors; D014170:Transformation, Genetic
chemical_list: D005656:Fungal Proteins; D012331:RNA, Fungal; D002374:Catalase; D010100:Oxygen