title: Novel adsorbent for DNA adsorption: Fe(3+)-attached sporopollenin particles embedded composite cryogels.
abstract: The aim of this study is to prepare supermacroporous cryogels embedded with Fe(3+)-attached sporopollenin particles (Fe(3++)-ASPs) having large surface area for high DNA adsorption capacity. Supermacroporous poly(2-hydroxyethyl methacrylate) (PHEMA)-based monolithic cryogel column embedded with Fe3+(+)-ASPs was prepared by radical cryo-copolymerization of 2-hydroxyethyl methacrylate (HEMA) with N,N´-methylene- bis-acrylamide (MBAAm) as cross-linker directly in a plastic syringe for DNA adsorption studies. Firstly, Fe3+(+) ions were attached to the sporopollenin particles (SPs), then the supermacroporous PHEMA cryogel with embedded Fe(3++)-ASPs was produced by free radical polymerization using N,N,N´, N´-Tetramethylethylenediamine (TEMED) and ammonium persulfate (APS) as initiator/activator pair in an ice bath. Optimum conditions of adsorption experiments were performed at pH 6.0 (0.02 M Tris buffer containing 0.2 M NaCl), with flow rate of 0.5 mL/min, and at 5°C. The maximum amount of DNA adsorption from aqueous solution was very high (109 mg/g SPs) with initial concentration of 3 mg/mL. It was observed that DNA could be repeatedly adsorbed and desorbed with this composite cryogel without significant loss of adsorption capacity. As a result, higher amounts of DNA adsorbed these composite cryogels are expected to be good candidate for achieving higher removal of anti-DNA antibodies from systemic lupus erythematosus (SLE) patients plasma.
authors.affiliation: Chemistry Department, Biochemistry Division, Aksaray University , Aksaray , Turkey.
authors.forename: Şeyda
authors.initials: Ş
authors.lastname: Ceylan
authors.forename: Mehmet
authors.initials: M
authors.lastname: Odabaşı
mesh_terms: D000327:Adsorption; D001704:Biopolymers / Q000737:chemistry*; D002338:Carotenoids / Q000737:chemistry*; D002846:Chromatography, Affinity; D059985:Cryogels / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000737:chemistry*; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry; D013499:Surface Properties; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D001704:Biopolymers; D059985:Cryogels; D014867:Water; C009800:sporopollenin; D011102:Polyhydroxyethyl Methacrylate; D002338:Carotenoids; D004247:DNA; D007501:Iron