title: A high-throughput template for optimizing Drosophila organ culture with response-surface methods.
abstract: The Drosophila wing imaginal disc is a key model organ for molecular developmental genetics. Wing disc studies are generally restricted to end-point analyses of fixed tissues. Recently several studies have relied on limited data from discs cultured in uncharacterized conditions. Systematic efforts towards developing Drosophila organ culture techniques are becoming crucial for further progress. Here, we have designed a multi-tiered, high-throughput pipeline that employs design-of-experiment methods to design a culture medium for wing discs. The resulting formula sustains high levels of proliferation for more than 12 hours. This approach results in a statistical model of proliferation as a function of extrinsic growth supplements and identifies synergies that improve insulin-stimulated growth. A more dynamic view of organogenesis emerges from the optimized culture system that highlights important facets of growth: spatiotemporal clustering of cell divisions and cell junction rearrangements. The same approach could be used to improve culture conditions for other organ systems.
authors.affiliation: Institute of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, Zurich CH-8057, Switzerland. konrad.basler@imls.uzh.ch
authors.forename: Jeremiah
authors.initials: J
authors.lastname: Zartman
authors.forename: Simon
authors.initials: S
authors.lastname: Restrepo
authors.forename: Konrad
authors.initials: K
authors.lastname: Basler
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods* / Q000592:standards; D002455:Cell Division; D002460:Cell Line; D049109:Cell Proliferation; D003470:Culture Media / Q000378:metabolism / Q000592:standards*; D004330:Drosophila / Q000166:cytology* / Q000378:metabolism; D057166:High-Throughput Screening Assays / Q000379:methods / Q000592:standards; D060227:Imaginal Discs / Q000166:cytology* / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D007814:Larva / Q000166:cytology / Q000378:metabolism; D009924:Organ Culture Techniques / Q000379:methods / Q000592:standards; D044967:Serum / Q000378:metabolism; D059008:Time-Lapse Imaging; D014921:Wings, Animal / Q000166:cytology / Q000378:metabolism
chemical_list: D003470:Culture Media; D007328:Insulin