title: [In vivo screening of illegal drug].
abstract: The neuro-behavioral observation scorebook that improved the previous observation methods of Irwin was followed, the test material was administered to 5 mice per each group, and the mean value of the obtained score was determined. The behavior of a normal animal was assumed to be point 0, animals showing suppressive behavior were scored in the minus region, and animals that showed excitement behavior were scored in the plus region. Each score was divided into three stages, according to the level of strength of the biological effect. The score of each observation item was totaled, and the level of the strength of the biological effect in the item was judged according to its mean value. These test methods of neuro-behavioral observations we proposed were able to detect the biological effects of a drug simply and promptly, and contributed sufficient data to support an administrative measure aimed at anticipating and improving the prevention of health damage in humans by non-regulated drugs from a scientific perspective. Recently, we developed a method of serial measurement of the quantity of monoamine in the mouse central nervous system by microdialysis, and performed it. The Kanagawa Prefectural Institute of Public Health conducted a study of the biological effect of non-regulated drugs. A characteristic here is what they examined about drug-dependency other than observing the behavior of the animal.
authors.affiliation: Tokyo Metropolitan Institute of Public Health, Japan. Akio_Ogata@member.metro.tokyo.jp
authors.forename: Akio
authors.initials: A
authors.lastname: Ogata
authors.forename: Kanako
authors.initials: K
authors.lastname: Satoh
authors.forename: Tatsu
authors.initials: T
authors.lastname: Fuwa
authors.forename: Toyohito
authors.initials: T
authors.lastname: Tanaka
authors.forename: Akemichi
authors.initials: A
authors.lastname: Nagasawa
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Yuzawa
authors.forename: Norio
authors.initials: N
authors.lastname: Yano
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ando
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Kubo
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takahashi
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Ohyama
authors.forename: Maki
authors.initials: M
authors.lastname: Miyazawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Kojima
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D015306:Biogenic Monoamines / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006801:Humans; D013287:Illicit Drugs / Q000009:adverse effects* / Q000494:pharmacology; D017551:Microdialysis; D009043:Motor Activity / Q000187:drug effects; D009420:Nervous System / Q000187:drug effects*; D019966:Substance-Related Disorders / Q000209:etiology / Q000517:prevention & control
chemical_list: D015306:Biogenic Monoamines; D013287:Illicit Drugs