title: Production and partial characterization of cellulases and Xylanases from Trichoderma atroviride 676 using lignocellulosic residual biomass.
abstract: Trichoderma atroviride 676 was studied to evaluate its efficiency in the production of some lignocellulolytic enzymes, using lignocellulosic residual biomass. Best results were obtained when 3.0 % (w/v) untreated sugarcane bagasse was used (61.3 U mL(-1) for xylanase, 1.9 U mL(-1) for endoglucanase, 0.25 U mL(-1) for FPase, and 0.17 U mL(-1) for β-glucosidase) after 3-4 days fermentation. The maximal enzymatic activity for endoglucanase, FPase, and xylanase were observed at 50-60 °C and pH 4.0-5.0, whereas thermal stability at 50 °C (CMCase and FPase) or 40 °C (xylanase) was obtained after 8 h. Zymograms have shown two bands of 104 and 200 kDa for endoglucanases and three bands for xylanase (23, 36, and 55.7 kDa). The results obtained with T. atroviride strain 676 were comparable to those obtained with the cellulolytic strain Trichoderma reesei RUT-C30, indicating, in the studied conditions, its great potential for biotechnological application, especially lignocellulose biomass hydrolysis.
authors.affiliation: Departamento de Microbiologia Geral, Instituto de Microbiologia Prof. Paulo de Góes, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-590, Brazil.
authors.forename: André Luiz
authors.initials: AL
authors.lastname: Grigorevski-Lima
authors.forename: Mariana Menezes Quadros
authors.initials: MM
authors.lastname: de Oliveira
authors.forename: Rodrigo Pires
authors.initials: RP
authors.lastname: do Nascimento
authors.forename: Elba Pinto da Silva
authors.initials: EP
authors.lastname: Bon
authors.forename: Rosalie Reed Rodrigues
authors.initials: RR
authors.lastname: Coelho
mesh_terms: D018533:Biomass; D044602:Cellulases / Q000378:metabolism*; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism*; D031786:Saccharum / Q000378:metabolism; D014242:Trichoderma / Q000201:enzymology*
chemical_list: C036909:lignocellulose; D008031:Lignin; D044602:Cellulases; D043364:Endo-1,4-beta Xylanases