title: Expression, purification, crystallization and preliminary X-ray diffraction analysis of Arabidopsis thaliana Deg8.
abstract: Arabidopsis thaliana Deg8, an ATP-independent serine endopeptidase, is involved in the repair of photosystem II (PSII), specifically the degradation of the photo-damaged PSII reaction centre D1 protein. To understand the molecular mechanism underlying the participation of Deg8 in the degradation of the photo-damaged D1 protein, the structure of Deg8 is needed. Until recently, however, no structure of Deg8 had been solved. In this study, Deg8 from A. thaliana was cloned, overexpressed and purified in Escherichia coli. Crystallization was performed at 277 K using tribasic sodium citrate as the precipitant and the crystals diffracted to 2.0 Å resolution, belonging to space group C2 with unit-cell parameters a = 129.5, b = 124.2, c = 93.3 Å, α = γ = 90, β = 132.4°. Assuming one trimer in the asymmetric unit, the Matthews coefficient and the solvent content were calculated to be 2.35 Å(3) Da(-1) and 47.6%, respectively.
authors.affiliation: Laboratory of Noncoding RNA, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, People's Republic of China.
authors.forename: Xiaoyue
authors.initials: X
authors.lastname: Shan
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Haitian
authors.initials: H
authors.lastname: Fan
authors.forename: Minze
authors.initials: M
authors.lastname: Jia
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Weimin
authors.initials: W
authors.lastname: Gong
mesh_terms: D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D003001:Cloning, Molecular; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D009154:Mutation; D011487:Protein Conformation; D012697:Serine Endopeptidases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D029681:Arabidopsis Proteins; C576657:Deg8 protein, Arabidopsis; D012697:Serine Endopeptidases