title: Trend of dengue virus infection at Lucknow, north India (2008- 2010): a hospital based study.
abstract: OBJECTIVE
  Dengue virus (DV) infection has emerged as a major health problem in north India. Here, we report the annual trend of dengue virus infection as seen in Lucknow, Uttar Pradesh, during 2008-2010.
  
  
  METHODS
  Blood samples from clinically suspected cases of dengue virus infection were collected and history was taken on structured clinical data sheet. All samples were tested for dengue IgM by antibody capture ELISA. Selected samples were tested by conventional RT-PCR for dengue virus RNA. Weather information was continuously recorded from website of world weather information service .
  
  
  RESULTS
  There was a gradual increase in number of dengue fever cases with increased occurrence in 2010. Cases referred in January - December 2008 were 398 (54.5% anti DV IgM positive), in January - December 2009 were 599 (51.9% anti DV IgM positive) and in January - December 2010 were 1602 (64.9% anti DV IgM positive). Serotypes circulating in years 2008, 2009 and 2010 were DV-2 & DV-3, DV -1, 2 & 3 and DV-1 and DV-2 respectively. There is no statistical significant correlation between weather data and increasing dengue positive cases.
  
  
  CONCLUSIONS
  Increased cases of dengue fever were seen in 2010, which was not correlated with any change in environmental factors. A change in circulating serotypes was noted.
authors.affiliation: Department of Microbiology, King George's Medical University, Lucknow, India.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Pandey
authors.forename: Rachna
authors.initials: R
authors.lastname: Nagar
authors.forename: Shikha
authors.initials: S
authors.lastname: Gupta
authors.forename: Danish
authors.initials: D
authors.lastname: Khan
authors.forename: Desh Deepak
authors.initials: DD
authors.lastname: Singh
authors.forename: Gitika
authors.initials: G
authors.lastname: Mishra
authors.forename: Shantanu
authors.initials: S
authors.lastname: Prakash
authors.forename: K P
authors.initials: KP
authors.lastname: Singh
authors.forename: Mastan
authors.initials: M
authors.lastname: Singh
authors.forename: Amita
authors.initials: A
authors.lastname: Jain
mesh_terms: D003715:Dengue / Q000453:epidemiology*; D003716:Dengue Virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D006761:Hospitals; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D007194:India / Q000453:epidemiology; D012367:RNA, Viral / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D007075:Immunoglobulin M; D012367:RNA, Viral