title: Differential expression of estradiol and estrogen receptor α in severe             preeclamptic pregnancies compared with normal pregnancies.
abstract: The purpose of this study was to investigate the expression of estradiol             and estrogen receptor α (ESRα) in severe preeclamptic (sPE) pregnancies compared             with normal pregnancies. Sera and placentas were obtained from i) patients with             sPE (n=25) and ii) a normal control group (n=25) who underwent elective Cesarean             deliveries. Estradiol expression was assessed by enzyme-linked immunosorbent assays             (ELISAs). ESRα expression was assessed by reverse transcription polymerase chain             reaction (RT-PCR) analysis and western blot analysis. In preeclamptic pregnancies,             estradiol was underexpressed (P<0.05), however, ESRα mRNA and protein levels             were increased significantly in comparison with normal pregnancies (P<0.05).             These results show that estradiol and ESRα are deregulated in preeclamptic pregnancies,             which in turn suggests the involvement of these molecules in the pathogenesis             of preeclampsia.
authors.affiliation: Department of Obstetrics and Gynecology, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shanxi 710038, PR China.
authors.forename: Guowu
authors.initials: G
authors.lastname: Yin
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhu
authors.forename: Chun
authors.initials: C
authors.lastname: Guo
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Tao
authors.initials: T
authors.lastname: Han
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Wen
authors.initials: W
authors.lastname: Yin
authors.forename: Ping
authors.initials: P
authors.lastname: Gao
authors.forename: Huizhong
authors.initials: H
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Geng
authors.forename: Julei
authors.initials: J
authors.lastname: Wang
authors.forename: Liang
authors.initials: L
authors.lastname: Liang
mesh_terms: D000328:Adult; D002824:Chorionic Villi / Q000378:metabolism; D004958:Estradiol / Q000097:blood*; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D010920:Placenta / Q000378:metabolism; D011225:Pre-Eclampsia / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D047628:Estrogen Receptor alpha; D012333:RNA, Messenger; D004958:Estradiol