title: Metabolism and protein transformations in synovial membrane of a knee joint in the course of rheumatoid arthritis and degenerative arthritis.
abstract: BACKGROUND
  In the course of musculoskeletal system diseases, such as rheumatoid arthritis and degenerative arthritis a chronic inflammatory process develops, which deteriorates all the joint elements and leads to the movement insufficiency of a patient. In case of both of theses diseases, etiology is multi-factor and still not known thoroughly. It is suggested that in the process of degradation of a joint cartilage, active form of oxygen take part. Their excessive production contributes to oxidation imbalances in cells and an oxidative stress. Under the activity of fee radicals, among others, activation of proteolytic enzymes participating the collagen degradation starts. The aim of this work is to compare parameters characteristic a cell metabolism and protein transformations taking place in the course of the aforementioned musculoskeletal system diseases.
  
  
  METHODS
  The material tested consisted of fragments of synovial membrane of a knee joint taken from 36 women suffering from rheumatoid arthritis and 24 women suffering from osteoarthritis during the procedure of knee-joint endoprothesoplastic surgery. Then the material was subject to the author's methodology of preparations of synovial membrane for biochemical markings.
  
  
  RESULTS
  In the group of patients suffering from rheumatoid arthritis significantly higher protein and sulfhydryl groups concentrations were achieved. Moreover, an increase of activity of manganese isoenzyme of superoxide dismutase, glutamate dehydrogenase and enzymes participating in the process of collagen degradation--prolidase and acid phosphatase was observed.
  
  
  CONCLUSIONS
  In the course of rheumatoid arthritis a speed of cell metabolism increase, which leads to a higher intensity of protein turnover in cells.
authors.affiliation: Institute of General Biochemistry of the Chair of Biochemistry, Medical University of Silesia, Zabrze, Poland. beata_hapeta@wp.pl
authors.forename: Beata
authors.initials: B
authors.lastname: Hapeta
authors.forename: Bogdan
authors.initials: B
authors.lastname: Koczy
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Fitowska
authors.forename: Michał
authors.initials: M
authors.lastname: Dobrakowski
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Kasperczyk
authors.forename: Alina
authors.initials: A
authors.lastname: Ostałowska
authors.forename: Tomasz
authors.initials: T
authors.lastname: Stołtny
authors.forename: Wojciech
authors.initials: W
authors.lastname: Widuchowski
authors.forename: Piotr
authors.initials: P
authors.lastname: Łukasik
authors.forename: Ewa
authors.initials: E
authors.lastname: Birkner
authors.forename: Sławomir
authors.initials: S
authors.lastname: Kasperczyk
mesh_terms: D000368:Aged; D001172:Arthritis, Rheumatoid / Q000201:enzymology / Q000378:metabolism* / Q000601:surgery; D015415:Biomarkers / Q000378:metabolism; D024043:Collagen Type II / Q000378:metabolism; D005260:Female; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000201:enzymology / Q000378:metabolism* / Q000601:surgery; D012307:Risk Factors; D013482:Superoxide Dismutase / Q000378:metabolism; D013582:Synovial Fluid / Q000201:enzymology / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D024043:Collagen Type II; D013482:Superoxide Dismutase; D005969:Glutamate Dehydrogenase