title: Generation and neuronal differentiation of induced pluripotent stem cells in Cdyl-/- mice.
abstract: Chromodomain on Y-like (CDYL) is a chromodomain protein that has sequence homology to members of the enoyl CoA hydratase family. Although the chromodomain of CDYL has been implicated in chromatin remodeling during mammalian spermatogenesis, the function of the Cdyl gene remains unclear. Recently, induced pluripotent stem cells (iPS cells) have been derived from somatic cells by the forced expression of several transcription factors. iPS cells resemble embryonic stem cells in many respects. Therefore, iPS cells represent a powerful tool for the study of gene function. In this study, we have investigated whether iPS cells derived from Cdyl-/- and Cdyl+/+ fibroblasts have different characteristics. Our results showed that both Cdyl-/- and Cdyl+/+ fibroblasts could be induced to become iPS cells, but the spontaneous neuronal differentiation capacity of Cdyl-/- iPS cells was much greater than that of the Cdyl+/+ iPS cells. These results provide some insight into the molecular function of the Cdyl gene, showing that it inhibited the neuronal differentiation of iPS cells.
authors.affiliation: Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-sen University, Guangzhou 510080, People’s Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Wan
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Hu
authors.forename: Sun-Xing
authors.initials: SX
authors.lastname: Yan
authors.forename: Fei
authors.initials: F
authors.lastname: Chen
authors.forename: Bing
authors.initials: B
authors.lastname: Cai
authors.forename: Xiu-Ming
authors.initials: XM
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Xin-Bing
authors.initials: XB
authors.lastname: Yu
authors.forename: Andy Peng
authors.initials: AP
authors.lastname: Xiang
authors.forename: Wei-Qiang
authors.initials: WQ
authors.lastname: Li
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D056970:Co-Repressor Proteins; D004622:Embryo, Mammalian; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D051548:Histone Acetyltransferases; D006836:Hydro-Lyases; D057026:Induced Pluripotent Stem Cells / Q000502:physiology*; D051379:Mice; D008819:Mice, Nude; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D012878:Skin Neoplasms / Q000209:etiology / Q000473:pathology; D013724:Teratoma / Q000209:etiology / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D056970:Co-Repressor Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D051548:Histone Acetyltransferases; C485563:Cdyl protein, mouse; D006836:Hydro-Lyases