title: Clinical utility of high-throughput glycome analysis in patients with pancreatic cancer.
abstract: BACKGROUND
  Most of the glycan changes reported in cancers were based on the examinations of a small number of patients or particular proteins. The aim of this study was to determine the changes of the serum N-glycan profile comprehensively in a large number of pancreatic cancer patients and investigate its clinical utility.
  
  
  METHODS
  Glycan levels in the serum of 92 pancreatic cancer patients and 243 healthy volunteers (HLT) were examined by comprehensive quantitative high-throughput glycome analysis and were compared with clinical parameters.
  
  
  RESULTS
  Out of 66 glycans detected, 15 were differentially expressed in pancreatic cancer, and 10 out of the 15 glycans were significantly up-regulated in cases with distant metastasis. There was a clear increase in overall expression of serum glycans, especially highly-branched glycans with fucose moieties, in pancreatic cancer. Among these 15 glycans, a tri-antennary complex type glycan (m/z 3195) showed the highest area under the receiver operating characteristic curve (AUROC = 0.799) for the diagnosis of pancreatic cancer. The ratio of pairs of glycans on the same path of the biosynthesis pathway (m/z 3195/1914) was found to be significantly higher in pancreatic cancer than in HLT (median = 1.11 and 0.41, respectively; p < 0.0001, AUROC = 0.831). For this pair ratio, the hazard ratio for survival (2.60, 95 % CI = 1.44-4.79) was higher than that of any single glycan and 1-year survival of patients with a high and low ratio was 36.9 and 69.2 %, respectively, (p = 0.001).
  
  
  CONCLUSIONS
  Comprehensive glycome analysis can be used to know the presence of pancreatic cancer, distant metastasis, and patient prognosis, simultaneously.
authors.affiliation: Department of Molecular Hepatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, Okayama, 700-8558, Japan, nouso@cc.okayama-u.ac.jp.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nouso
authors.forename: Maho
authors.initials: M
authors.lastname: Amano
authors.forename: Yoichi M
authors.initials: YM
authors.lastname: Ito
authors.forename: Koji
authors.initials: K
authors.lastname: Miyahara
authors.forename: Yuki
authors.initials: Y
authors.lastname: Morimoto
authors.forename: Hironari
authors.initials: H
authors.lastname: Kato
authors.forename: Koichiro
authors.initials: K
authors.lastname: Tsutsumi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tomoda
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Nakamura
authors.forename: Sayo
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Kenji
authors.initials: K
authors.lastname: Kuwaki
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Hagihara
authors.forename: Hideki
authors.initials: H
authors.lastname: Onishi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Miyake
authors.forename: Fusao
authors.initials: F
authors.lastname: Ikeda
authors.forename: Hidenori
authors.initials: H
authors.lastname: Shiraha
authors.forename: Akinobu
authors.initials: A
authors.lastname: Takaki
authors.forename: Taku
authors.initials: T
authors.lastname: Nakahara
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Nishimura
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D005260:Female; D064368:Healthy Volunteers; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000097:blood*; D011134:Polysaccharides / Q000032:analysis* / Q000097:blood; D011379:Prognosis; D012372:ROC Curve; D016019:Survival Analysis
chemical_list: D014408:Biomarkers, Tumor; D011134:Polysaccharides