title: A signature for induced pluripotent stem cell-associated genes in colorectal cancer.
abstract: Genes associated with induced pluripotent stem cells (iPS genes) are several pivotal transcriptional factors, which are used to induce pluripotent stem cells from some adult somatic cells. The roles of these iPS genes and especially the signature for these iPS genes in colorectal cancer (CRC) are still unclear. Overexpressed Oct4 and Lin28 but down-regulated Nanog were found in tumor tissues compared with that in their paired normal counterparts of CRC patients. Interestingly, we found that Oct4, Lin28 and Nanog were highly overexpressed in some patients. And the signature for iPS genes was correlated with tumor site (P = 0.012), lymph node status (P = 0.033), Dukes classification (P = 0.033) of CRC patients. Moreover, an independent public expression profiling data showed signature for the four iPS genes could successfully be used to predict the survival of CRC patients with Dukes stages B and C. Immunofluorescent staining of fresh CRC tissues from patients showed that strong co-expressions of Oct4 and Nanog proteins or Sox2 and Lin28 were present in some CRC cells. Then, CRC cell subclone with four iPS genes overexpression were establish by a mixed retroviral system. We found that iPS genes promote sphere-formation, proliferation, colony formation, migration of human CRC cells in vitro and tumor growth in vivo. Our study first shows the clinical significance of iPS signature in CRC patients.
authors.affiliation: Department of Pathology, Baoan Hospital, Southern Medical University, Shenzhen 518101, People's Republic of China. pathologylee@126.com
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Xun-Hua
authors.initials: XH
authors.lastname: Liu
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Sui
authors.forename: Yong-Xia
authors.initials: YX
authors.lastname: Liu
authors.forename: Zheng-Quan
authors.initials: ZQ
authors.lastname: Xiao
authors.forename: Ping
authors.initials: P
authors.lastname: Zheng
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Su
authors.initials: S
authors.lastname: Yao
authors.forename: Cheng
authors.initials: C
authors.lastname: Xing
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Li
mesh_terms: D015153:Blotting, Western; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D018572:Disease-Free Survival; D005260:Female; D005455:Fluorescent Antibody Technique; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D000071317:Nanog Homeobox Protein; D009367:Neoplasm Staging; D050814:Octamer Transcription Factor-3 / Q000235:genetics*; D039904:Pluripotent Stem Cells; D012333:RNA, Messenger / Q000032:analysis; D016601:RNA-Binding Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D059467:Transcriptome
chemical_list: D018398:Homeodomain Proteins; C000715187:Lin28A protein, human; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D012333:RNA, Messenger; D016601:RNA-Binding Proteins