title: Simultaneous determination and pharmacokinetic study of metformin and pioglitazone in dog plasma by LC-MS-MS.
abstract: A high-performance liquid chromatography-tandem mass spectrometry method was developed for the simultaneous determination of metformin (MET) and pioglitazone (PIO) in dogs. To increase the reliability of the method, moroxydine (IS-1) and rosiglitazone (IS-2) were used as internal standards for MET and PIO, respectively. The analytes were extracted from beagle dog plasma by a one-step protein precipitation. Chromatographic separation was performed on a Phenomenex Synergi POLAR-RP 80A column (250 × 4.6 mm, 4 µm). The total chromatographic run time was 8.0 min, with retention times of 3.0 and 2.9 min for MET and IS-1, respectively, and 6.1 and 4.9 min for PIO and IS-2. The lower limit of quantitation values were 2 and 1 ng/mL for MET and PIO, respectively. The recoveries ranged from 96.4 to 112.8% for MET and 102.1 to 104.5% for PIO.
authors.affiliation: 1Key Lab of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, 210009, PR China.
authors.forename: Xueyuan
authors.initials: X
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.forename: Ping
authors.initials: P
authors.lastname: Wan
authors.forename: Lifang
authors.initials: L
authors.lastname: Yin
authors.forename: Guangji
authors.initials: G
authors.lastname: Wang
authors.forename: Jianguo
authors.initials: J
authors.lastname: Sun
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004285:Dogs; D004355:Drug Stability; D005260:Female; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D008687:Metformin / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D000077205:Pioglitazone; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D045162:Thiazolidinediones / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics
chemical_list: D045162:Thiazolidinediones; D008687:Metformin; D000077205:Pioglitazone