title: [Mass spectrometry detection of monomeric renalase in human urine].
abstract: Renalase is a recently discovered secretory protein, which is suggested to play a role (which still remains elusive) in regulation of blood pressure. Earlier it was purified from urine of healthy volunteers by means of ammonium sulfate fractionation and subsequent affinity chromatography (Xu et al. (2005) J. Clin. Invest., 115, 1275). The resultant purified preparation of renalase contained 2 proteins with molecular masses of 35 and 67-75 kDa. The authors believed that the latter represents a dimerization (aggregation) product of the 35 kDa protein. In this study we have detected relanase in urinary samples of 2 of 6 volunteers only after immunoaffinity enrichment of urinary samples subjected to ammonium sulfate precipitation. Electrophoresis of the purified preparation also demonstrated the presence of 2 proteins with molecular masses of 35 and 66 kDa, respectively. Mass spectrometry analysis of these proteins identified 35 and 66 kDa proteins as renalase and serum albumin, respectively. Thus, our results do not support suggestion on formation of renalase dimers and they indicate that urinary renalase excretion significantly varies in humans.
authors.forename: V I
authors.initials: VI
authors.lastname: Fedchenko
authors.forename: O A
authors.initials: OA
authors.lastname: Buneeva
authors.forename: A T
authors.initials: AT
authors.lastname: Kopylov
authors.forename: A A
authors.initials: AA
authors.lastname: Kaloshin
authors.forename: L N
authors.initials: LN
authors.lastname: Aksenova
authors.forename: V G
authors.initials: VG
authors.lastname: Zgoda
authors.forename: A E
authors.initials: AE
authors.lastname: Medvedev
mesh_terms: D000419:Albuminuria / Q000652:urine; D019281:Dimerization; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008875:Middle Aged; D008995:Monoamine Oxidase / Q000652:urine*; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000378:metabolism
chemical_list: D012709:Serum Albumin; D008995:Monoamine Oxidase; C521557:renalase