title: A "novel" protocol for the analysis of hydroxycinnamic acids in leaf tissue of chicory (Cichorium intybus L., Asteraceae).
abstract: A "novel" protocol is presented for easy and reliable estimation of soluble hydroxycinnamate levels in Cichorium intybus L. leaf tissue in large-scale experiments. Samples were standardized by punching 6 discs per leaf, and hydroxycinnamates were extracted by submerging the discs in 80% ethanol with 5% acetic acid for at least 48 h in the darkness at 4°C. Residual dry mass of the discs was used for a posteriori correction of compound levels. Chlorophyll was eliminated by chloroform, and the aqueous phases were transferred to microplates, dried, and dissolved in 50% methanol for HPLC analysis and storage. An HPLC program of 8 min was developed for the analysis of the extracts. Comparisons with extractions of liquid nitrogen powders indicated that the novel extraction method was reliable. No degradation of the major hydroxycinnamates-caftaric, chlorogenic, and chicoric acids-was observed, during maceration at ambient temperatures, or after storage for 1 year.
authors.affiliation: Université Lille 1, Sciences et Technologies, UMR INRA 1281 "Stress Abiotiques et Différenciation des Végétaux Cultivés", GIS GENOCHIC, Bâtiment SN2, 3ème Étage, 59655 Villeneuve d'Ascq Cedex, France.
authors.forename: Meriem
authors.initials: M
authors.lastname: Bahri
authors.forename: Philippe
authors.initials: P
authors.lastname: Hance
authors.forename: Sébastien
authors.initials: S
authors.lastname: Grec
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Quillet
authors.forename: Francis
authors.initials: F
authors.lastname: Trotin
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Hilbert
authors.forename: Theo
authors.initials: T
authors.lastname: Hendriks
mesh_terms: D019659:Asteraceae; D002626:Chemistry, Pharmaceutical / Q000379:methods / Q000639:trends*; D018651:Cichorium intybus; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D003373:Coumaric Acids / Q000032:analysis* / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D018515:Plant Leaves
chemical_list: D003373:Coumaric Acids