title: Protection against UVB-induced oxidative stress in human skin cells and skin models by methionine sulfoxide reductase A.
abstract: Environmental trauma to human skin can lead to oxidative damage of proteins and affect their activity and structure. When methionine becomes oxidized to its sulfoxide form, methionine sulfoxide reductase A (MSRA) reduces it back to methionine. We report here the increase in MSRA in normal human epidermal keratinocytes (NHEK) after ultraviolet B (UVB) radiation, as well as the reduction in hydrogen peroxide levels in NHEK pre-treated with MSRA after exposure. Further, when NHEK were pre-treated with a non-cytotoxic pentapeptide containing methionine sulfoxide (metSO), MSRA expression increased by 18.2%. Additionally, when the media of skin models were supplemented with the metSO pentapeptide and then exposed to UVB, a 31.1% reduction in sunburn cells was evident. We conclude that the presence of MSRA or an externally applied peptide reduces oxidative damage in NHEK and skin models and that MSRA contributes to the protection of proteins against UVB-induced damage in skin.
authors.affiliation: Estee Lauder Research Laboratories, Melville, NY 11747, USA. epelle@estee.com
authors.forename: Edward
authors.initials: E
authors.lastname: Pelle
authors.forename: Daniel
authors.initials: D
authors.lastname: Maes
authors.forename: Xi
authors.initials: X
authors.lastname: Huang
authors.forename: Krystyna
authors.initials: K
authors.lastname: Frenkel
authors.forename: Nadine
authors.initials: N
authors.lastname: Pernodet
authors.forename: Daniel B
authors.initials: DB
authors.lastname: Yarosh
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D015194:Diffusion Chambers, Culture; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D008715:Methionine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D051150:Methionine Sulfoxide Reductases / Q000378:metabolism*; D008954:Models, Biological; D009842:Oligopeptides / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects / Q000528:radiation effects; D018384:Oxidative Stress; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D017768:Skin, Artificial; D046509:Tissue Culture Techniques; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D009842:Oligopeptides; D011837:Radiation-Protective Agents; D008715:Methionine; D006861:Hydrogen Peroxide; D051150:Methionine Sulfoxide Reductases; C027219:methionine sulfoxide reductase; C013111:methionine sulfoxide