title: [Heterologous expression of stearoyl-CoA desaturase-1 in Lactococcus lactis NZ3900].
abstract: The possibility of heterologous expression of human Stearoyl-CoA Desaturase (scd1) was investigated. The scd1 encoding sequence was inserted into the pNZ8149 to generate the pNZ8149-scd1 expression plasmids. Then we introduced the pNZ8149-scd1 construct into the Lactococcus lactis NZ3900 to investigate its enzyme activity. The results show that heterologous expressed SCD1 enzyme resulted in a 92%-169% increase in the C16:1n-7 and a 53-127% increase in the C18:1n-7 (P<0.05). The SCD1 enzyme was capable of producing n-7 fatty acids in Lactococcus lactis efficiently. It also suggests that the fatty acid desaturases can be heterologous expressed in Lactococcus lactis to produce the helpful fatty acids.
authors.affiliation: College Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, Gansu, China.
authors.forename: Lamei
authors.initials: L
authors.lastname: Wang
authors.forename: Shili
authors.initials: S
authors.lastname: Li
authors.forename: Kemian
authors.initials: K
authors.lastname: Gou
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Luo
mesh_terms: D018274:Electroporation; D006801:Humans; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D016254:Mutagenesis, Insertional; D009561:Nisin / Q000494:pharmacology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013230:Stearoyl-CoA Desaturase / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D009561:Nisin; D013230:Stearoyl-CoA Desaturase