title: Rapid methods for the extraction and archiving of molecular grade fungal genomic DNA.
abstract: The rapid and inexpensive extraction of fungal genomic DNA that is of sufficient quality for molecular approaches is central to the molecular identification, epidemiological analysis, taxonomy, and strain typing of pathogenic fungi. Although many commercially available and in-house extraction procedures do eliminate the majority of contaminants that commonly inhibit molecular approaches, the inherent difficulties in breaking fungal cell walls lead to protocols that are labor intensive and that routinely take several hours to complete. Here we describe several methods that we have developed in our laboratory that allow the extremely rapid and inexpensive preparation of fungal genomic DNA.
authors.affiliation: Mycology Reference Laboratory, Health Protection Agency, South-West Regional Laboratory, Bristol, UK. andy.borman@uhbristol.nhs.uk
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Borman
authors.forename: Michael
authors.initials: M
authors.lastname: Palmer
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Johnson
mesh_terms: D018070:Biological Specimen Banks; D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D046508:Culture Techniques; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification*; D005374:Filtration / Q000295:instrumentation / Q000379:methods*; D005658:Fungi / Q000235:genetics / Q000254:growth & development; D016681:Genome, Fungal / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D004271:DNA, Fungal