title: Simultaneous determination of triterpenoid saponins in dog plasma by a validated UPLC-MS/MS and its application to a pharmacokinetic study after administration of total saponin of licorice.
abstract: A rapid and sensitive ultra performance liquid chromatography-tandem mass spectrometry method was developed for simultaneous determination of eight constituents, uralsaponin C, uralsaponin F, 22β-acetoxyl-glycyrrhizin, 24-hydroxy-licorice-saponin E2, licorice-saponin G2, licorice-saponin E2, glycyrrhizin, and licorice-saponin J2, from total saponin of licorice (TSL) in dog plasma. Ardisiacrispin A was used as the internal standard (IS). The separation was performed on Thermo Syncronic C₁₈ column (100 mm × 2.1 mm, 1.7 μm) at a flow rate of 0.4 mL min⁻¹, and acetonitrile/methanol (3:1, v/v)-0.1% formic acid was used as mobile phase. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) via electrospray ionization (ESI) source with negative ionization mode. All calibration curves had good linearity (r>0.991) over the concentration range from 2.03-405 ng mL⁻¹ to 2.63-2625 ng mL⁻¹ for all components. The intra- and inter-day precision was within 15% and the accuracy ranged from -14.08% to 13.8%. The method was successfully applied to pharmacokinetic study of eight triterpenoid saponins in dog plasma after oral administration of TSL.
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Tao
authors.forename: Jinao
authors.initials: J
authors.lastname: Duan
authors.forename: Jianming
authors.initials: J
authors.lastname: Guo
authors.forename: Jianping
authors.initials: J
authors.lastname: Li
authors.forename: Yuping
authors.initials: Y
authors.lastname: Tang
authors.forename: Pei
authors.initials: P
authors.lastname: Liu
authors.forename: Nianyun
authors.initials: N
authors.lastname: Yang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000737:chemistry* / Q000493:pharmacokinetics; D002138:Calibration; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000493:pharmacokinetics; D020834:Ethnopharmacology; D029882:Glycyrrhiza uralensis / Q000737:chemistry; D057230:Limit of Detection; D008297:Male; D018517:Plant Roots / Q000737:chemistry*; D011489:Protein Denaturation; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry*; D012503:Saponins / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D014315:Triterpenes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D012503:Saponins; D014315:Triterpenes