title: Increased neuronal activity fragments the Golgi complex.
abstract: The Golgi complex is essential for many aspects of cellular function, including trafficking and sorting of membrane and secretory proteins and posttranslational modification by glycosylation. We observed reversible fragmentation of the Golgi complex in cultured hippocampal neurons cultured in hyperexcitable conditions. In addition, Golgi fragmentation was found in cultured neurons with hyperactivity due to prolonged blockade of GABA(A)-mediated inhibition or withdrawal of NMDA receptor antagonism. The interplay between neuronal hyperactivity and Golgi structure established in this study thus reveals a previously uncharacterized impact of neuronal activity on organelle structure. This finding may have important roles in protein processing and trafficking in the Golgi as well as effects on neuronal signaling.
authors.affiliation: Department of Physiology, and Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA.
authors.forename: Desiree A
authors.initials: DA
authors.lastname: Thayer
authors.forename: Yuh Nung
authors.initials: YN
authors.lastname: Jan
authors.forename: Lily Yeh
authors.initials: LY
authors.lastname: Jan
mesh_terms: D015763:2-Amino-5-phosphonovalerate / Q000494:pharmacology; D000818:Animals; D001596:Benzylamines / Q000494:pharmacology; D001640:Bicuculline / Q000494:pharmacology; D020013:Calcium Signaling; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D002478:Cells, Cultured; D055724:Electrophysiological Phenomena; D004789:Enzyme Activation; D058787:GABA-A Receptor Antagonists / Q000494:pharmacology; D006056:Golgi Apparatus / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors; D013449:Sulfonamides / Q000494:pharmacology; D000096926:Benzenesulfonamides
chemical_list: D015763:2-Amino-5-phosphonovalerate; D001596:Benzylamines; D001640:Bicuculline; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D058787:GABA-A Receptor Antagonists; D009419:Nerve Tissue Proteins; D011188:Potassium; D016194:Receptors, N-Methyl-D-Aspartate; D013449:Sulfonamides; C072105:KN 93; D000096926:Benzenesulfonamides