title: Laboratory methods and biomarkers in the evaluation of bisphosphonate effects on body tissues: a literature review.
abstract: Bisphosphonates are extensively used to treat bone metabolism disorders; meanwhile, these drugs have important side effects such as jaw osteonecrosis and femoral fracture. Therefore, studies have been conducted aimed at better understanding their mechanism of action and determining a course of action to avoid these side effects. We present here a literature review focusing on the laboratory methods available for investigating bisphosphonate effects on body tissues. There are many different methods available for this purpose, but the appropriate application of these methods and knowledge of their limitations are crucial for understanding the effects of bisphosphonates.
authors.affiliation: Postgraduate Program, Dental College, Pontifical Catholic University of Rio Grande do Sul - PUCRS, Porto Alegre, RS, Brazil.
authors.forename: Ana Carolina Uchoa
authors.initials: AC
authors.lastname: Vasconcelos
authors.forename: Soraya
authors.initials: S
authors.lastname: de Azambuja Berti-Couto
authors.forename: Maria Antonia
authors.initials: MA
authors.lastname: Figueiredo
authors.forename: Fernanda Gonçalves
authors.initials: FG
authors.lastname: Salum
authors.forename: Tiago Giuliani
authors.initials: TG
authors.lastname: Lopes
authors.forename: Karen
authors.initials: K
authors.lastname: Cherubini
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D050071:Bone Density Conservation Agents / Q000494:pharmacology*; D001842:Bone and Bones / Q000187:drug effects; D019411:Clinical Laboratory Techniques; D004164:Diphosphonates / Q000494:pharmacology*; D006801:Humans; D018919:Neovascularization, Physiologic / Q000187:drug effects
chemical_list: D015415:Biomarkers; D050071:Bone Density Conservation Agents; D004164:Diphosphonates
keywords: biomarkers; bisphosphonates; body tissues; laboratory methods