title: Flow cytometric analysis of STAT phosphorylation.
abstract: Flow cytometry can be used to study STAT phosphorylation on a per cell basis. Cells are fixed, permeablized, and stained with antibodies that specifically bind to the phosphorylated form of the STAT protein. This allows the tyrosine phosphorylation of a single STAT to be studied within a heterogeneous cell population and/or the phosphorylation of several STATs within the one cell type.
authors.affiliation: Reid Rheumatology Laboratory, Inflammation Division, Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
authors.forename: Jane
authors.initials: J
authors.lastname: Murphy
authors.forename: Gabrielle L
authors.initials: GL
authors.lastname: Goldberg
mesh_terms: D005434:Flow Cytometry / Q000379:methods*; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D050796:STAT3 Transcription Factor / Q000737:chemistry / Q000378:metabolism*; D050799:STAT5 Transcription Factor / Q000737:chemistry / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism
chemical_list: D050796:STAT3 Transcription Factor; D050799:STAT5 Transcription Factor; D016179:Granulocyte Colony-Stimulating Factor; D014443:Tyrosine; D016178:Granulocyte-Macrophage Colony-Stimulating Factor