title: Effects of 300 mT static magnetic field on IL-6 secretion in normal human colon myofibroblasts.
abstract: Intestinal subepithelial myofibroblasts play crucial role in the growth and development of the intestine. Colitis, small bowel injury, gastric ulcer disease and inflammatory bowel disease (IBD) accompany the increase of number of activated myofibroblasts. In the last few years, the increasing production of electromagnetic (EMF) and static magnetic fields (SMF), due to the expanding use of electronic devices in everyday life, has led to a number of studies on the effects of these fields on living organisms. EMF therapy, because of its anti-inflammatory properties, may be used in medicine in IBD treatment. This mechanism has not been elucidated yet. In the present work normal human colon myofibroblasts CCD-18Co were exposed to SMF with a flux density of 300 mT. After 24 h incubation TNF-alpha-dependent IL-6 secretion was determined with ELISA kit (RandD Systems).The influence of magnetic field and its effect on cell proliferation were determined with TOX-2 (In Vitro Toxicology Assay Kit XTT Based, TOX-2, Sigma) and CyQUANT NF cell proliferation assay kit (Molecular Probes). It was shown that SMF inhibited TNF-alpha-dependent IL-6 secretion. The observed effects were statistically significant and depended on the time of incubation. Moreover, SMF triggered cell proliferation whereas it did not alter cell viability. IL-6 belongs to pro-inflammatory cytokines family and plays a crucial role in IBD. Inhibition of IL-6 secretion by SMF and lack of its cytotoxic effect seem to be advantageous whilst SMF is implicated in the treatment of inflammatory diseases associated by increase in number of activated myofibroblasts.
authors.affiliation: Department of Biopharmacy, Medical University of Silesia, Sosnowiec, Poland. agruchlik@sum.edu.pl
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Gruchlik
authors.forename: Adam
authors.initials: A
authors.lastname: Wilczok
authors.forename: Ewa
authors.initials: E
authors.lastname: Chodurek
authors.forename: Władysław
authors.initials: W
authors.lastname: Polechoński
authors.forename: Daniel
authors.initials: D
authors.lastname: Wolny
authors.forename: Zofia
authors.initials: Z
authors.lastname: Dzierzewicz
mesh_terms: D002478:Cells, Cultured; D003106:Colon / Q000378:metabolism / Q000528:radiation effects*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D060526:Magnetic Fields; D058628:Myofibroblasts / Q000378:metabolism / Q000528:radiation effects*
chemical_list: D015850:Interleukin-6