title: Antiviral activity of phosvitin from zebrafish Danio rerio.
abstract: Lymphocystis disease virus (LCDV), a virus of Iridoviridae, can infect numerous teleost species, causing serious losses of aquaculture industry, and thus effective ways of prophylaxis and treatment are demanded. Previous studies have shown that phosvitin (Pv) is an antimicrobial agent in zebrafish, and vitellogenin, the precursor of yolk proteins including Pv, is able to neutralize virus, we thus hypothesize that Pv may have an antiviral activity. Here we clearly demonstrated that recombinant Pv (rPv) purified was capable of inhibiting the cytopathic effect in LCDV-infected cells and reducing the virus quantities in the infected cells as well as in the infected zebrafish. These data indicate that Pv possesses an antiviral activity and participates in immune defense of host against the infection by viruses like LCDV.
authors.affiliation: Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity and Department of Marine Biology, Ocean University of China, Qingdao 266003, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Sun
authors.forename: Lili
authors.initials: L
authors.lastname: Hu
authors.forename: Shousheng
authors.initials: S
authors.lastname: Liu
authors.forename: Guobin
authors.initials: G
authors.lastname: Hu
authors.forename: Shicui
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000276:immunology / Q000378:metabolism*; D002460:Cell Line; D019610:Cytoprotection; D004266:DNA Virus Infections / Q000276:immunology*; D007113:Immunity, Innate; D007497:Iridoviridae / Q000276:immunology*; D010774:Phosvitin / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D019562:Viral Load / Q000276:immunology; D014819:Vitellogenins / Q000276:immunology / Q000378:metabolism; D015027:Zebrafish / Q000276:immunology
chemical_list: D000890:Anti-Infective Agents; D011994:Recombinant Proteins; D014819:Vitellogenins; D010774:Phosvitin