title: Evaluation of three turbidimetric assays for automated determination of hemoglobin A1c.
abstract: BACKGROUND
  To compare the results of HbA1c determination obtained through immunoassays versus the HPLC method currently used routinely in our laboratory.
  
  
  METHODS
  We evaluated immunoturbidimetric assays for the HbA1c measure on three analyzers, specifically the Roche Cobas Integra 400+ (Roche Diagnostics, Indianapolis, IN, USA), Ortho Clinical Diagnostics Vitros 5.1 FS (Ortho Clinical Diagnostics, NY, USA), and Siemens Dimension RxL (Siemens Healthcare Diagnostics, NY, USA), in comparison with the HPLC Menarini HA 8140 (Menarini Diagnostics, Rungis, France) currently used in our laboratory.
  
  
  RESULTS
  Analytical performances including precision, analytical range, recovery, carryover, erythrosedimentation and comparison studies were acceptable leading to results with a level of exactitude in accordance with the recommendations of the National Glycohemoglobin Standardization Program (NGSP).
  
  
  CONCLUSIONS
  The three immunoassays tested can be used interchangeably and will be satisfactory for laboratories who cannot invest in a HPLC analyzer.
authors.affiliation: Department of Biochemistry, Lapeyronie Hospital, Montpellier, France.
authors.forename: Amandine
authors.initials: A
authors.lastname: Barrot
authors.forename: Anne Marie
authors.initials: AM
authors.lastname: Dupuy
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Badiou
authors.forename: Anne Sophie
authors.initials: AS
authors.lastname: Bargnoux
authors.forename: Jean Paul
authors.initials: JP
authors.lastname: Cristol
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D006442:Glycated Hemoglobin; C517652:hemoglobin A1c protein, human