title: Short term interactions with long term consequences: modulation of chimeric vessels by neural progenitors.
abstract: Vessels are a critical and necessary component of most tissues, and there has been substantial research investigating vessel formation and stabilization. Several groups have investigated coculturing endothelial cells with a second cell type to promote formation and stabilization of vessels. Some have noted that long-term vessels derived from implanted cocultures are often chimeric consisting of both host and donor cells. The questions arise as to whether the coculture cell might impact the chimeric nature of the microvessels and can modulate the density of donor cells over time. If long-term engineered microvessels are primarily of host origin, any impairment of the host's angiogenic ability has significant implications for the long-term success of the implant. If one can modulate the host versus donor response, one may be able to overcome a host's angiogenic impairment. Furthermore, if one can modulate the donor contribution, one may be able to engineer microvascular networks to deliver molecules a patient lacks systemically for long times. To investigate the impact of the cocultured cell on the host versus donor contributions of endothelial cells in engineered microvascular networks, we varied the ratio of the neural progenitors to endothelial cells in subcutaneously implanted poly(ethylene glycol)/poly-L-lysine hydrogels. We found that the coculture of neural progenitors with endothelial cells led to the formation of chimeric host-donor vessels, and the ratio of neural progenitors has a significant impact on the long term residence of donor endothelial cells in engineered microvascular networks in vivo even though the neural progenitors are only present transiently in the system. We attribute this to the short term paracrine signaling between the two cell types. This suggests that one can modulate the host versus donor contributions using short-term paracrine signaling which has broad implications for the application of engineered microvascular networks and cellular therapy more broadly.
authors.affiliation: Interdepartmental Neuroscience Program, Yale University, New Haven, Connecticut, United States of America.
authors.forename: Cicely
authors.initials: C
authors.lastname: Williams
authors.forename: Millicent Ford
authors.initials: MF
authors.lastname: Rauch
authors.forename: Michael
authors.initials: M
authors.lastname: Michaud
authors.forename: Rebecca
authors.initials: R
authors.lastname: Robinson
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.forename: Joseph
authors.initials: J
authors.lastname: Madri
authors.forename: Erin
authors.initials: E
authors.lastname: Lavik
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000166:cytology*; D004730:Endothelium, Vascular / Q000166:cytology*; D005260:Female; D006801:Humans; D020100:Hydrogels; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology*; D023822:Tissue Engineering
chemical_list: D020100:Hydrogels