title: Seroreactivity to new Mycobacterium leprae protein antigens in different leprosy-endemic regions in Brazil.
abstract: New Mycobacterium leprae protein antigens can contribute to improved serologic tests for leprosy diagnosis/classification and multidrug therapy (MDT) monitoring. This study describes seroreactivity to M. leprae proteins among participants from three highly endemic leprosy areas in Brazil: central-western Goiânia/Goiás (GO) (n = 225), Rondonópolis/Mato Grosso (MT) (n = 764) and northern Prata Village/Pará (PA) (n = 93). ELISA was performed to detect IgG to proteins (92f, 46f, leprosy IDRI diagnostic-1, ML0405, ML1213) and IgM to phenolic glycolipid-I (PGL-I). Multibacillary (MB) leprosy had positive rates for PGL-I that were similar to those for proteins; however, some anti-PGL-I-negative subjects were positive for proteins, suggesting that adding protein antigen to PGL-I can enhance the sensitivity of MB leprosy detection. In MT, different degrees of seroreactivity were observed and ranked for MB, former patients after MDT, paucibacillary (PB) leprosy, household contact (HHC) and endemic control (EC) groups. The seroreactivity of PB patients was low in GO and MT. HHCs from different endemic sites had similar IgG antibody responses to proteins. 46f and 92f were not recognised by most tuberculosis patients, ECs or HHCs within GO, an area with high BCG vaccination coverage. Low positivity in EC and HHC was observed in PA and MT. Our results provide evidence for the development of an improved serologic test that could be widely applicable for MB leprosy testing in Brazil.
authors.affiliation: Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: Emerith Mayra
authors.initials: EM
authors.lastname: Hungria
authors.forename: Regiane Morillas de
authors.initials: RM
authors.lastname: Oliveira
authors.forename: Ana Lúcia Osório Maroclo de
authors.initials: AL
authors.lastname: Souza
authors.forename: Maurício Barcelos
authors.initials: MB
authors.lastname: Costa
authors.forename: Vânia Nieto Brito de
authors.initials: VN
authors.lastname: Souza
authors.forename: Eliane Aparecida
authors.initials: EA
authors.lastname: Silva
authors.forename: Fátima Regina Vilani
authors.initials: FR
authors.lastname: Moreno
authors.forename: Maria Esther Salles
authors.initials: ME
authors.lastname: Nogueira
authors.forename: Maria Renata Sales Nogueira
authors.initials: MR
authors.lastname: Costa
authors.forename: Sônia Maria Usó Ruiz
authors.initials: SM
authors.lastname: Silva
authors.forename: Samira
authors.initials: S
authors.lastname: Bührer-Sékula
authors.forename: Steven G
authors.initials: SG
authors.lastname: Reed
authors.forename: Malcolm S
authors.initials: MS
authors.lastname: Duthie
authors.forename: Mariane Martins de Araújo
authors.initials: MM
authors.lastname: Stefani
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000097:blood*; D001426:Bacterial Proteins / Q000097:blood*; D001938:Brazil / Q000453:epidemiology; D016022:Case-Control Studies; D019353:Endemic Diseases; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006017:Glycolipids / Q000097:blood*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D007918:Leprosy / Q000175:diagnosis* / Q000453:epidemiology; D008297:Male; D009166:Mycobacterium leprae / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D006017:Glycolipids; D007075:Immunoglobulin M; C045169:phenolic glycolipid I, Mycobacterium leprae