title: [Complete genome sequencing and sequence analysis of BCG Tice].
abstract: OBJECTIVE
  The objective of this study is to obtain the complete genome sequence of Bacillus Calmette-Guerin Tice (BCG Tice), in order to provide more information about the molecular biology of BCG Tice and design more reasonable vaccines to prevent tuberculosis.
  
  
  METHODS
  We assembled the data from high-throughput sequencing with SOAPdenovo software, with many contigs and scaffolds obtained. There are many sequence gaps and physical gaps remained as a result of regional low coverage and low quality. We designed primers at the end of contigs and performed PCR amplification in order to link these contigs and scaffolds. With various enzymes to perform PCR amplification, adjustment of PCR reaction conditions, and combined with clone construction to sequence, all the gaps were finished.
  
  
  RESULTS
  We obtained the complete genome sequence of BCG Tice and submitted it to GenBank of National Center for Biotechnology Information (NCBI). The genome of BCG Tice is 4334064 base pairs in length, with GC content 65.65%.
  
  
  CONCLUSIONS
  The problems and strategies during the finishing step of BCG Tice sequencing are illuminated here, with the hope of affording some experience to those who are involved in the finishing step of genome sequencing. The microarray data were verified by our results.
authors.affiliation: Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. zhiming.w.com@163.com
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Wang
authors.forename: Yuanlong
authors.initials: Y
authors.lastname: Pan
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.forename: Baoli
authors.initials: B
authors.lastname: Zhu
mesh_terms: D001500:BCG Vaccine / Q000235:genetics*; D001483:Base Sequence; D020451:Contig Mapping; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D001500:BCG Vaccine