title: Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH.
abstract: We developed a fluorescence in situ hybridization (FISH) method for the rapid detection of Proteus spp. in urine, using a novel peptide nucleic acid (PNA) probe. Testing on 137 urine samples from patients with urinary tract infections has shown specificity and sensitivity values of 98 % (95 % CI, 93.2-99.7) and 100 % (95 % CI, 80,8-100), respectively, when compared with CHROMagar Orientation medium. Results indicate that PNA-FISH is a reliable alternative to traditional culture methods and can reduce the diagnosis time to approximately 2 h.
authors.affiliation: IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal. carinaalmeida@deb.uminho.pt
authors.forename: C
authors.initials: C
authors.lastname: Almeida
authors.forename: N F
authors.initials: NF
authors.lastname: Azevedo
authors.forename: J C
authors.initials: JC
authors.lastname: Bento
authors.forename: N
authors.initials: N
authors.lastname: Cerca
authors.forename: H
authors.initials: H
authors.lastname: Ramos
authors.forename: M J
authors.initials: MJ
authors.lastname: Vieira
authors.forename: C W
authors.initials: CW
authors.lastname: Keevil
mesh_terms: D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D020135:Peptide Nucleic Acids; D011511:Proteus / Q000235:genetics* / Q000302:isolation & purification; D011512:Proteus Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D014552:Urinary Tract Infections / Q000175:diagnosis* / Q000382:microbiology*; D014556:Urine / Q000382:microbiology
chemical_list: D020135:Peptide Nucleic Acids