title: Real-time chemical measurements of dopamine release in the brain.
abstract: Rapid changes in extracellular dopamine concentrations in freely moving or anesthetized rats can be detected using fast-scan cyclic voltammetry (FSCV). Background-subtracted FSCV is a real-time electrochemical technique that can monitor neurochemical transmission in the brain on a subsecond timescale, while providing chemical information on the analyte. Also, this voltammetric approach allows for the investigation of the kinetics of release and uptake of molecules in the brain. This chapter describes, completely, how to make these measurements and the properties of FSCV that make it uniquely suitable for performing chemical measurements of dopaminergic neurotransmission in vivo.
authors.affiliation: Department of Chemistry, North Carolina State University, Raleigh, NC, USA.
authors.forename: James G
authors.initials: JG
authors.lastname: Roberts
authors.forename: Leyda Z
authors.initials: LZ
authors.lastname: Lugo-Morales
authors.forename: Philip L
authors.initials: PL
authors.lastname: Loziuk
authors.forename: Leslie A
authors.initials: LA
authors.lastname: Sombers
mesh_terms: D000758:Anesthesia; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000601:surgery; D002244:Carbon / Q000737:chemistry; D000077482:Carbon Fiber; D004298:Dopamine / Q000378:metabolism*; D004558:Electric Stimulation; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D008839:Microelectrodes; D051381:Rats; D012984:Software; D013997:Time Factors
chemical_list: D000077482:Carbon Fiber; D002244:Carbon; D004298:Dopamine