title: Bioactive lipids metabolites in Amanita virosa.
abstract: Thrombin is the key serine proteinase of the coagulation cascade and, therefore, a suitable target for inhibition of blood coagulation. An extract of Amanita virosa considerably inhibited thrombin (48%), but showed no inhibitory activity on trypsin. On the basis of inhibition selectivity between thrombin and trypsin and potency of thrombin inhibition, A. virosa constitutes a good starting material for the isolation of further compounds that are active against thrombin. Bioassay oriented fractionation of the extract of A. virosa led to the isolation of a complex mixture of triglycerides (TGs), monoacylglycerols (MAGs), free fatty acids (FAs) and ergosterol. The structures of the isolated lipids metabolites were determined on the basis of chemical and spectroscopic evidences.
authors.affiliation: Department of Chemistry and Pharmaceutical Sciences, P.zle Europa 1, 34127, University of Trieste, Trieste, Italy. cateni@units.it
authors.forename: Francesca
authors.initials: F
authors.lastname: Cateni
authors.forename: Marina
authors.initials: M
authors.lastname: Zacchigna
authors.forename: Bojan
authors.initials: B
authors.lastname: Doljak
authors.forename: Marko
authors.initials: M
authors.lastname: Anderluh
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Procida
authors.forename: Andrej
authors.initials: A
authors.lastname: Piltaver
mesh_terms: D000545:Amanita / Q000737:chemistry* / Q000378:metabolism; D000991:Antithrombins / Q000302:isolation & purification*; D004875:Ergosterol / Q000302:isolation & purification; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure
chemical_list: D000991:Antithrombins; D008055:Lipids; D004875:Ergosterol