title: Evaluation of hematologic, blood gas, and select biochemical variables in ovine whole blood stored in CPDA-1 bags.
abstract: BACKGROUND
  The economic consequences from mortality of sheep after blood loss can be considerable. To date there are no reports addressing hematologic, blood gas, and biochemical changes in ovine blood stored in CPDA-1 bags.
  
  
  OBJECTIVE
  The aim of this study was to investigate hematologic, blood gas, and biochemical alterations resulting from storage of ovine blood in CPDA-1 bags to establish transfusion protocols in sheep.
  
  
  METHODS
  From each of 7 healthy 8-month-old sheep 450 mL of blood were collected into CPDA-1 bags and stored for 35 days in at 3-6°C. Samples were taken from the bags at days 0, 7, 14, 21, and 35. Whole blood was used to assess PCV, MCV, RBC count, pH, pO2 , pCO2 , and concentrations of bicarbonate, sodium, and lactate. Plasma was used to measure potassium, hemoglobin, and glucose concentrations.
  
  
  RESULTS
  The PCV remained stable throughout the storage period, while plasma hemoglobin and MCV began to increase on days 7 and 21, respectively. The RBC count began to decrease on day 21. Blood pH decreased and pCO2 increased steadily throughout the storage period. Potassium concentration increased from 3.8 to 18.3 mmol/L on day 7 and remained high thereafter. In contrast, sodium concentration began to decrease on day 7.
  
  
  CONCLUSIONS
  The results show that ovine blood undergoes hematologic, blood gas, and biochemical changes during storage. Further studies are required to establish RBC viability in CPDA-1 bags after a storage period of 35 days.
authors.affiliation: Federal Rural University of Semiarid, Mossoró, RN, Brazil.
authors.forename: Rejane S
authors.initials: RS
authors.lastname: Sousa
authors.forename: Raimundo A
authors.initials: RA
authors.lastname: Barrêto
authors.forename: Isadora K F
authors.initials: IK
authors.lastname: Sousa
authors.forename: Dowglish F
authors.initials: DF
authors.lastname: Chaves
authors.forename: Herbert S
authors.initials: HS
authors.lastname: Soares
authors.forename: Isabella O
authors.initials: IO
authors.lastname: Barros
authors.forename: Antonio H H
authors.initials: AH
authors.lastname: Minervino
authors.forename: Enrico L
authors.initials: EL
authors.lastname: Ortolani
mesh_terms: D000225:Adenine / Q000494:pharmacology*; D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D001774:Blood Chemical Analysis / Q000662:veterinary; D001784:Blood Gas Analysis / Q000662:veterinary; D001793:Blood Preservation / Q000662:veterinary*; D002951:Citrates / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D006403:Hematologic Tests / Q000662:veterinary; D010710:Phosphates / Q000494:pharmacology*; D019064:Product Packaging; D012756:Sheep / Q000097:blood*; D013048:Specimen Handling / Q000662:veterinary*
chemical_list: D000925:Anticoagulants; C034876:CPDA solutions; D002951:Citrates; D010710:Phosphates; D005947:Glucose; D000225:Adenine