title: [Expression of metallothionein 2 in middle ear cholesteatoma and its correlation with interleukin 1α and interleukin 6].
abstract: OBJECTIVE
  To detect the expression and distribution of metallothionein 2 (MT-2) in middle ear cholesteatoma, and to explore the relationship of MT-2 with interleukin 1α (IL-1α) and interleukin 6 (IL-6), and well as to explore the role of MT-2 in the pathogenetic mechanism of middle ear cholesteatoma.
  
  
  METHODS
  Using the immunohistochemistry and reverse transcription polymerase chain reaction to examine the expressions of MT-2, IL-1α and IL-6 protein and MT-2 mRNA in twenty-five middle ears' cholesteatoma and seven normal external ears' canal skin. The influence of cholesteatoma debris on the MT-2 mRNA and protein of HaCaT cell were further analyzed.
  
  
  RESULTS
  According to immunohistochemistry research, the expressions of MT-2, IL-1α and IL-6 were extremely higher in middle ear cholesteatoma than those in normal external ears' canal skin (P < 0.05). The result of RT-PCR showed that there was significant difference between the expression of MT-2 mRNA of and middle ear cholesteatoma than those in normal external ear canal skin (t = 15.38, P < 0.05). There was a positive correlation between the expression of MT-2 and that of IL-1α (r = 0.856, P < 0.05). There was also positive correlation between the expression of MT-2 and that of IL-6 (r = 0.714, P < 0.05). MT-2 mRNA and protein of HaCaT cell significantly increased when exposed to cholesteatoma debris in a dose dependent manner.
  
  
  CONCLUSIONS
  MT-2, IL-1α and IL-6 may play an important role in the pathogenetic process of middle ear cholesteatoma. Cholesteatoma debris may involve in the proliferation of middle ear cholesteatoma by activation of MT-2.
authors.affiliation: Department of Otorhinolaryngology, China-Japan Friendship Hospital, Beijing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Lin-lin
authors.initials: LL
authors.lastname: Yu
authors.forename: Jun
authors.initials: J
authors.lastname: Han
authors.forename: Jing-ning
authors.initials: JN
authors.lastname: Cheng
authors.forename: Da-zhang
authors.initials: DZ
authors.lastname: Yang
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D018424:Cholesteatoma, Middle Ear / Q000378:metabolism*; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D008297:Male; D008668:Metallothionein / Q000378:metabolism*; D008875:Middle Aged; D055815:Young Adult
chemical_list: C508600:IL6 protein, human; D007375:Interleukin-1; D015850:Interleukin-6; D008668:Metallothionein