title: Effects of epithelial cell injury of the lower respiratory tract in the pathogenesis of allergic responses in a rat model.
abstract: BACKGROUND
  Asthma is a complex disease involving genetic and environment interactions. Atopy is a strong risk factor for asthma. The airway epithelium not only forms a physical barrier but also provides immune defense against harmful materials. To explore the effects of airway epithelium on asthma, we hypothesized that environmental injuries could act on bronchial epithelial cells and damage the physical barrier, which might facilitate allergens to stimulate immunoreactions and play an important role in the pathogenesis of asthma.
  
  
  METHODS
  Thirty eight-week-old male Wistar rats were randomly divided into five groups with six rats in each group: control group, asthma group, ovalbumin (OVA) + OVA group, lipopolysaccharide (LPS) group and LPS + OVA group. In the control group, 0.9% saline was injected intraperitoneally on day 1. Fourteen days later, the rats were exposed to aerosolized 0.9% saline. In the asthma group, the rats were sensitized with an injection of 10 mg of OVA, followed by an aerosolized 2% OVA challenge 14 days later. The OVA + OVA group was sensitized by an inhalation 2% OVA, 20 minutes a day, from day 1 to day 7, and then OVA challenged in the same way as the asthma group. In the LPS group, LPS (200 µl, 1 µg/µl) was given by airway on day 1 and day 3, with a simultaneous aerosol inhalation of 2% OVA for 20 minutes a day from day 1 to day 7. Fourteen days later, the rats were challenged with saline as in the control group. While in the LPS + OVA group, LPS (200 µl, 1 µg/µl) was given by airway on day 1 and day 3, with a simultaneous aerosol inhalation of 2% OVA for 20 minutes a day from day 1 to day 7. Fourteen days later, the rats were challenged with OVA as in the asthma group. The expression of interleukin (IL)-4, interferon-gamma (IFN-γ) and thymic stromal lymphopoietin (TSLP) in the lungs was detected by reverse transcription polymerase chain reaction (RT-PCR) and the pulmonary pathological changes were also observed. The level of IL-4, IFN-γ and IgE in plasma was detected by enzyme-linked immunosorbent assay (ELISA). Bronchoalveolar lavage fluid (BALF) was collected to conduct differential cell counts. Flow cytometry analysis was also used to count Th1 and Th2 cells.
  
  
  RESULTS
  The pathological changes in the LPS + OVA group were similar to the asthma group, while in other groups, the pathological changes were not obvious. The ratio of lymphocytes in BALF, IL-4/IFN-γ in plasma and the expression of the TSLP and IL-4 in the asthma and LPS + OVA groups were higher than in the control group and the OVA + OVA group (P < 0.05). The level of IgE was higher in the asthma, LPS and LPS + OVA groups than in the control group and the OVA + OVA group (P < 0.05). By flow cytometry analysis, the Th1/Th2 ratio was lower in the LPS + OVA and asthma groups than in other groups (P < 0.05).
  
  
  CONCLUSIONS
  The experiment results show that the injury to the bronchial epithelial layer may be the initial event of allergic responses. This finding implies that a rational approach to therapeutics would be to increase the resistance of the airways to environmental injuries rather than concentrating on suppressing inflammation.
authors.affiliation: Department of Respiratory Medicine, Peking University First Hospital, Beijing 100034, China.
authors.forename: Wei-li
authors.initials: WL
authors.lastname: Chu
authors.forename: Ming
authors.initials: M
authors.lastname: Chu
authors.forename: Yue-dan
authors.initials: YD
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.forename: Can
authors.initials: C
authors.lastname: Zhao
authors.forename: Li
authors.initials: L
authors.lastname: Su
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiong
authors.forename: Tong-sheng
authors.initials: TS
authors.lastname: Yang
authors.forename: Ying-hong
authors.initials: YH
authors.lastname: Tao
authors.forename: Hai-chao
authors.initials: HC
authors.lastname: Li
mesh_terms: D000818:Animals; D001980:Bronchi / Q000473:pathology*; D002452:Cell Count; D016207:Cytokines / Q000502:physiology; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000473:pathology*; D006967:Hypersensitivity / Q000209:etiology*; D007073:Immunoglobulin E / Q000097:blood; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D010047:Ovalbumin / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma; D010047:Ovalbumin; D000094632:Thymic Stromal Lymphopoietin; C000721968:TSLP protein, rat