title: miR-9 modulates the expression of interferon-regulated genes and MHC class I molecules in human nasopharyngeal carcinoma cells.
abstract: The functions of miR-9 in some cancers are recently implicated in regulating proliferation, epithelial-mesenchymal transition (EMT), invasion and metastasis, apoptosis, and tumor angiogenesis, etc. miR-9 is commonly down-regulated in nasopharyngeal carcinoma (NPC), but the exact roles of miR-9 dysregulation in the pathogenesis of NPC remains unclear. Therefore, we firstly used miR-9-expressing CNE2 cells to determine the effects of miR-9 overexpression on global gene expression profile by microarray analysis. Microarray-based gene expression data unexpectedly demonstrated a significant number of up- or down-regulated immune- and inflammation-related genes, including many well-known interferon (IFN)-induced genes (e.g., IFI44L, PSMB8, IRF5, PSMB10, IFI27, PSB9_HUMAN, IFIT2, TRAIL, IFIT1, PSB8_HUMAN, IRF1, B2M and GBP1), major histocompatibility complex (MHC) class I molecules (e.g., HLA-B, HLA-C, HLA-F and HLA-H) and interleukin (IL)-related genes (e.g., IL20RB, GALT, IL7, IL1B, IL11, IL1F8, IL1A, IL6 and IL7R), which was confirmed by qRT-PCR. Moreover, the overexpression of miR-9 with the miRNA mimics significantly up- or down-regulated the expression of above-mentioned IFN-inducible genes, MHC class I molecules and IL-related genes; on the contrary, miR-9 inhibition by anti-miR-9 inhibitor in CNE2 and 5-8F cells correspondingly decreased or increased the aforementioned immune- and inflammation-related genes. Taken together, these findings demonstrate, for the first time, that miR-9 can modulate the expression of IFN-induced genes and MHC class I molecules in human cancer cells, suggesting a novel role of miR-9 in linking inflammation and cancer, which remains to be fully characterized.
authors.affiliation: Cancer Research Institute, Southern Medical University, Guangzhou 510515, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.forename: Zun-Lan
authors.initials: ZL
authors.lastname: Zhao
authors.forename: Wen-Tao
authors.initials: WT
authors.lastname: Zhao
authors.forename: Quan-Rong
authors.initials: QR
authors.lastname: Fan
authors.forename: Sheng-Chun
authors.initials: SC
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Yu-Qing
authors.initials: YQ
authors.lastname: Zhang
authors.forename: Jun-Wen
authors.initials: JW
authors.lastname: Shi
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Lin
authors.forename: Sheng
authors.initials: S
authors.lastname: Yang
authors.forename: Rao-Ying
authors.initials: RY
authors.lastname: Xie
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Zhang
authors.forename: Yong-Liang
authors.initials: YL
authors.lastname: Sun
authors.forename: Kang
authors.initials: K
authors.lastname: Xu
authors.forename: Kai-Tai
authors.initials: KT
authors.lastname: Yao
authors.forename: Dong
authors.initials: D
authors.lastname: Xiao
mesh_terms: D002277:Carcinoma; D015972:Gene Expression Regulation, Neoplastic; D005805:Genes, MHC Class I; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000276:immunology; D007372:Interferons / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D007372:Interferons