title: [Comparison of macrophages and dendritic cells infected by Brucella S(2)].
abstract: Objective  To make a comparison of the characteristics between macrophages and dendritic cells (DC) infected by Brucella suis (B. suis) S(2);.   Methods  Wrights-Giemsa's stainning was used to observe the cell morphology and calculate the phagocytic rate. ELISA was employed to detect the expressions of IL-12 and TNF-α in cell culture supernatants as well as the contents of IFN-γ and IL-4 in the co-culture with T cells. With annexin-V-FITC/PI double staining,  the cell apoptosis rate was determined by flow cytometry.   Results  1 h after infected by B. suis S(2);,  the phagocytic rate of macrophages was (43.6±4.8)%,  which was significantly higher than that of the DC (16.3±2.7)% (P<0.05). The apoptosis rates of normal macrophages and macrophages 6,  12 and 24 h after infected by B. suis S(2); were (3.09±1.21)%,  (19.89±1.36)%,  (22.73±2.21)% and (42.44±3.12)%,  respectively,  which were dramatically higher than those of the DC at the corresponding time points,  being (1.82±0.01)%,  (3.76±0.13)%,  (7.87±0.56)% and (9.08±0.23)%,  respectively (P<0.05). The levels of IL-12 secreted by macrophages 24 and 48 h after infected by B. suis S(2); were significantly lower than those by the DC (P<0.01). At 24,  48 and 72 h,  the levels of TNF-α secreted by macrophages were dramatically lower than those by the DC (P<0.01),  and the levels of IFN-γ in the co-culture supernatants of macrophages and T cells were significantly lower than those in DC and T cell co-culture (P<0.01).   Conclusion  Macrophages have a better ability in phagocytosing B. suis S(2); than DC and the apoptosis rate of macrophages is higher than that of DC after infected by B. suis S(2);,  but in activating and inducing the cellular immune response and presenting antigen,  DC are stronger than macrophages.
authors.affiliation: Department of Immunology, School of Basic Medicine, Hebei University, Baoding 071000, China. rongruixue_1234@163.com
authors.forename: Ruixue
authors.initials: R
authors.lastname: Rong
authors.forename: Bei
authors.initials: B
authors.lastname: Wang
authors.forename: Leifang
authors.initials: L
authors.lastname: Zhang
authors.forename: Weijiao
authors.initials: W
authors.lastname: Yan
authors.forename: Congyi
authors.initials: C
authors.lastname: Zheng
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Wang
authors.forename: Jiabo
authors.initials: J
authors.lastname: Ding
authors.forename: Kairong
authors.initials: K
authors.lastname: Mao
authors.forename: Zhiran
authors.initials: Z
authors.lastname: Cao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000276:immunology; D041021:Brucella suis / Q000276:immunology*; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D005260:Female; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D008297:Male; D051379:Mice
chemical_list: D016207:Cytokines