title: γ-Aminobutyric acid-mediated neurotransmission in cerebellar-hypothalamic circuit attenuates gastric mucosal injury induced by ischemia-reperfusion.
abstract: BACKGROUND
  Excessive greater splanchnic nerve (GSN) activation contributes to the progression of gastric ischemia-reperfusion (GI-R) injury. This study was designed to investigate the protective mechanism of cerebellar fastigial nucleus (FN) stimulation against GI-R injury.
  
  
  METHODS
  The GI-R injury model was induced in rats by clamping the celiac artery for 30 min, and then reperfusion for 30 min, 1, 3, 6, or 24 h, respectively.
  
  
  RESULTS
  Microinjection of L-Glu (3, 6, 12 μg) into the FN dose-dependently attenuated GI-R injury and GSN activity. In addition, there was an enhancement of gastric mucosal blood flow in GI-R rats. Pretreatment with the glutamic acid decarboxylase antagonist into the FN, the GABAA receptor antagonist into the lateral hypothalamic area or lesion of superior cerebellar peduncle all reversed the protective effects of the FN stimulation. Furthermore, the FN stimulation reduced the TUNEL-positive gastric mucosal cell and Bax-positive gastric mucosal cell in GI-R rats.
  
  
  CONCLUSIONS
  These results indicate that the protective effects of the FN stimulation against GI-R injury may be mediated by attenuation of the excessive GSN activation, gastric mucosal cell apoptosis, and Bax expression in GI-R rats.
authors.affiliation: Department of Physiology and Pathophysiology, Shanghai Medical College of Fudan University, Shanghai, China.
authors.forename: D S
authors.initials: DS
authors.lastname: Du
authors.forename: T
authors.initials: T
authors.lastname: Zhu
authors.forename: S T
authors.initials: ST
authors.lastname: Ren
authors.forename: G L
authors.initials: GL
authors.lastname: Xie
authors.forename: S B
authors.initials: SB
authors.lastname: Li
authors.forename: D C
authors.initials: DC
authors.lastname: Chu
authors.forename: X T
authors.initials: XT
authors.lastname: Liu
authors.forename: M
authors.initials: M
authors.lastname: Liu
authors.forename: X B
authors.initials: XB
authors.lastname: Ma
authors.forename: M H
authors.initials: MH
authors.lastname: Zhou
authors.forename: D N
authors.initials: DN
authors.lastname: Zhu
authors.forename: Z X
authors.initials: ZX
authors.lastname: Deng
authors.forename: J
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D002445:Celiac Artery / Q000473:pathology / Q000502:physiology; D002531:Cerebellum / Q000187:drug effects / Q000502:physiology*; D004558:Electric Stimulation / Q000379:methods; D018756:GABA Antagonists / Q000008:administration & dosage; D005753:Gastric Mucosa / Q000187:drug effects / Q000293:injuries* / Q000502:physiology; D005973:Glutamine / Q000008:administration & dosage; D007031:Hypothalamus / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008845:Microinjections / Q000379:methods; D009415:Nerve Net / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000503:physiopathology* / Q000517:prevention & control; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
chemical_list: D018756:GABA Antagonists; D005973:Glutamine; D005680:gamma-Aminobutyric Acid