title: The effect of mud-bath therapy on bone status in rats during adjuvant subchronic arthritis.
abstract: BACKGROUND
  We studied influence of mud-bath on bone status in male Wistar rats with subchronic arthritis.
  
  
  METHODS
  Arthritis was induced by 2 subplantar injections of Freund's adjuvans with heat-killed Streptoccocus pyogenes into paw. Groups: intact (int) on chippings; (con) arthritis on chippings; (san38) arthritis on hot sand; (mu38) arthritis on hot mud; (mu21) arthritis on mild mud. Bone mineral density (BMD, g/cm2) was measured by dual energy X-ray absorptiometry and femurs were tested biomechanically. Bone markers osteocalcin (OC), PINP and CTX were analysed in bone.
  
  
  RESULTS
  BMD of right femur decreased vs. left in san38 (p = 0.030) and mu38 (p = 0.047). Fracture load of right/left femur (N) decreased in experimental groups, significantly in san38 (p = 0.05). Fracture threshold of neck decreased in right vs. left in experimental groups, but significantly in san38 (p = 0.05). OC decreased in mu38 vs. con (1.84 +/- 0.14/2.62 +/- 0.23). PINP decreased in int vs. san38 (p = 0.005) and mu21 (p < 0.001). CTX decreased in int vs. mu38 (p = 0.006) and mu21 (p = 0.005).
  
  
  CONCLUSIONS
  The hot bath appears indifferent in relation to osteoporosis, while cold mud-bath shows good effect on bone metabolism. The cold mud-baths help to reduce arthritic inflammation and pain and thereby lead to higher mobility with positive consequence on bone.
authors.affiliation: Radioisotope Laboratories and Vivarium, Charles University in Prague, Faculty of Medicine, Hradec Králové, Czech Republic.
authors.forename: Helena
authors.initials: H
authors.lastname: Zivná
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Maric
authors.forename: Iveta
authors.initials: I
authors.lastname: Gradosová
authors.forename: Klára
authors.initials: K
authors.lastname: Svejkovská
authors.forename: Sona
authors.initials: S
authors.lastname: Hubená
authors.forename: Pavel
authors.initials: P
authors.lastname: Zivný
mesh_terms: D015502:Absorptiometry, Photon; D000818:Animals; D001169:Arthritis, Experimental / Q000473:pathology / Q000628:therapy*; D001696:Biomechanical Phenomena; D015519:Bone Density; D001842:Bone and Bones / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D009094:Mud Therapy; D051381:Rats; D017208:Rats, Wistar