title: [Effects of stress on corticosterone level, expression of c-Fos gene and serotonin turnover in brain in mice with genetic predisposition to catalepsy].
abstract: Freezing or catalepsy is a passive-defensive reaction to stress. The participation of brain serotonin (5-HT) in the regulation of catalepsy was shown. The major gene of predisposition to catalepsy in CBA strain was localized in a 59-70 cM fragment of chromosome 13. This fragment was transferred from the CBA strain to genome of AKR non-cataleptic strain and created AKR. CBA-D13Mit76 (D13) congenic strain. The aim of the study was to compare the effects of acute stress (restriction, 1 h) on corticosterone level in plasma, the expression of c-Fos gene (neuromarker of stress) and serotonin metabolism in the brain in AKR catalepsy-resistant strain and congenic D13 catalepsy-prone strain. The level of corticosterone was significantly lower (p < 0.001) in the stressed D13 mice compared with the stressed AKR mice. Acute stress led to increased expression of c-Fos gene in the hypothalamus and midbrain in mice of both strains. Stress increased (p < 0.05) serotonin turnover in midbrain in D13 mice, but not in AKR. Thus, the fragment of chromosome 13, containing the major gene of catalepsy, participates in the regulation of hormonal response and serotonin turnover to acute stress.
authors.forename: E Iu
authors.initials: EIu
authors.lastname: Bazhenova
authors.forename: A V
authors.initials: AV
authors.lastname: Kulikov
authors.forename: M A
authors.initials: MA
authors.lastname: Tikhonova
authors.forename: A S
authors.initials: AS
authors.lastname: Tsybko
authors.forename: N K
authors.initials: NK
authors.lastname: Popova
mesh_terms: D000818:Animals; D002375:Catalepsy / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D033481:Chromosomes, Mammalian; D003345:Corticosterone / Q000097:blood; D015870:Gene Expression; D020022:Genetic Predisposition to Disease; D007031:Hypothalamus / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D008636:Mesencephalon / Q000378:metabolism* / Q000503:physiopathology; D051379:Mice; D008806:Mice, Inbred AKR; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism*; D012149:Restraint, Physical / Q000009:adverse effects; D012701:Serotonin / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D012701:Serotonin; D003345:Corticosterone