title: TLR2 in pleural fluid is modulated by talc particles during pleurodesis.
abstract: The aim of this study was to examine the role of TLR2 molecule in pleural space during thoracoscopic talc pleurodesis period in patients with malignant pleural effusion. We analyzed TLR2 molecule in soluble form as well as on membrane of granulocytes in pleural fluid. Pleural fluid examination was done at three intervals during pleurodesis procedure: 1st-before the thoracoscopic procedure, 2nd-2 hours after the terminating thoracoscopic procedure with talc insufflation, 3rd-24 hours after the thoracoscopic procedure. We reported significant increase of soluble TLR2 molecule in pleural fluid effusion during talc pleurodesis from preoperative value. This increase was approximately 8-fold in the interval of 24 hours. The changes on granulocyte population were quite different. The mean fluorescent intensity of membrane TLR2 molecule examined by flow cytometry on granulocyte population significantly decreased after talc exposure with comparison to prethoracoscopic density. To estimate the prognostic value of TLR2 expression in pleural fluid patients were retrospectively classified into either prognostically favourable or unfavourable groups. Our results proved that patients with favourable prognosis had more than 3-fold higher soluble TLR2 level in pleural fluid early, 2 hours after talc pleurodesis intervention.
authors.affiliation: Department of Clinical Immunology and Allergy, Faculty of Medicine and University Hospital in Hradec Kralove, Charles University in Prague, Sokolska Street 581, 500 05 Hradec Kralove, Czech Republic. karolina.jankovicova@fnhk.cz
authors.forename: Karolina
authors.initials: K
authors.lastname: Jankovicova
authors.forename: Katerina
authors.initials: K
authors.lastname: Kondelkova
authors.forename: Petr
authors.initials: P
authors.lastname: Habal
authors.forename: Ctirad
authors.initials: C
authors.lastname: Andrys
authors.forename: Jan
authors.initials: J
authors.lastname: Krejsek
authors.forename: Jiri
authors.initials: J
authors.lastname: Mandak
mesh_terms: D000368:Aged; D005260:Female; D006098:Granulocytes / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007327:Insufflation / Q000379:methods; D008297:Male; D010994:Pleura / Q000187:drug effects / Q000378:metabolism*; D016066:Pleural Effusion, Malignant / Q000378:metabolism* / Q000628:therapy*; D018700:Pleurodesis / Q000379:methods*; D011379:Prognosis; D012189:Retrospective Studies; D013627:Talc / Q000008:administration & dosage*; D013906:Thoracoscopy / Q000379:methods; D051195:Toll-Like Receptor 2 / Q000378:metabolism*
chemical_list: C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2; D013627:Talc