title: P5-type sulfhydryl oxidoreductase promotes the sorting of proteins to protein body I in rice endosperm cells.
abstract: In rice (Oryza sativa) endosperm cells, oxidative protein folding is necessary for the sorting of storage proteins to protein bodies, PB-I and PB-II. Here we examined the role of sulfhydryl oxidoreductase PDIL2;3 (a human P5 ortholog) in the endoplasmic reticulum (ER), using GFP-AB, a PB-I marker in which the N-terminal region (AB) of α-globulin is fused to green fluorescent protein (GFP). RNAi knockdown of PDIL2;3 inhibited the accumulation of GFP-AB in PB-I and promoted its exit from the ER. We discuss the role of PDIL2;3 in retaining proteins within the ER and specifying their localization to PB-I through disulfide bond formation.
authors.affiliation: Division of Plant Sciences; National Institute of Agrobiological Sciences; Tsukuba, Ibaraki, Japan; Department of Food and Applied Life Sciences; Yamagata University; Tsuruoka, Yamagata, Japan.
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Onda
authors.affiliation: Division of Plant Sciences; National Institute of Agrobiological Sciences; Tsukuba, Ibaraki, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kawagoe
mesh_terms: D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D012275:Oryza / Q000378:metabolism*; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D010088:Oxidoreductases
keywords: endoplasmic reticulum; protein body; protein disulfide bond; protein storage vacuole; sulfhydryl oxidoreductase