title: Identification and typing of isolates of Cyphellophora and relatives by use of amplified fragment length polymorphism and rolling circle amplification.
abstract: The species diversity and identification of black fungi belonging to Cyphellophora and Phialophora, which colonize and infect human skin and nails, were studied using amplified fragment length polymorphism (AFLP). A total of 76 Cyphellophora and Phialophora isolates were evaluated, and their delimitation was compared to earlier studies using multilocus sequencing. The results of the AFLP analysis and sequencing were in complete agreement with each other. Seven species-specific padlock probes for the most prevalent species were designed on the basis of the ribosomal DNA internal transcribed spacer region, and identification of the respective species could easily be achieved with the aid of rolling circle amplification.
authors.affiliation: Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Peiying
authors.initials: P
authors.lastname: Feng
authors.forename: Corné H W
authors.initials: CH
authors.lastname: Klaassen
authors.forename: Jacques F
authors.initials: JF
authors.lastname: Meis
authors.forename: M J
authors.initials: MJ
authors.lastname: Najafzadeh
authors.forename: A H G
authors.initials: AH
authors.lastname: Gerrits Van Den Ende
authors.forename: Liyan
authors.initials: L
authors.lastname: Xi
authors.forename: G S
authors.initials: GS
authors.lastname: de Hoog
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D001203:Ascomycota / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D058889:Molecular Typing / Q000379:methods*; D016533:Mycological Typing Techniques / Q000379:methods*; D010676:Phialophora / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D017931:DNA Primers; D021903:DNA, Ribosomal Spacer