title: ADAM10 as a biomarker for Alzheimer's disease: a study with Brazilian elderly.
abstract: Alzheimer's disease (AD) is the most common cause of dementia in people above age 65. Platelet studies with ADAM10 have shown that its expression is reduced in AD patients. The aim of this research was to compare the platelet levels of ADAM10 protein in two Brazilian elderly groups, considering the stages of the disease. The SDS-PAGE technique followed by Western blotting was used. Data were analyzed using comparison, correlation and association statistical methods. The results showed reduced platelet ADAM10 levels in AD elderly compared to non-AD subjects. The disease progression intensified this reduction. ADAM10 was the only statistically significant variable (p = 0.01) to increase the AD occurrence probability. The cutoff value of 0.4212 in the receiver operating characteristic curve captured sensitivity and specificity of 70 and 80.77%, respectively. Together with other clinical criteria, ADAM10 seems to be a relevant biomarker tool for early and accurate AD diagnosis.
authors.affiliation: Departamento de Gerontologia, Universidade Federal de São Carlos, São Carlos, Brazil.
authors.forename: Patricia Regina
authors.initials: PR
authors.lastname: Manzine
authors.forename: Jessyka Maria
authors.initials: JM
authors.lastname: de França Bram
authors.forename: Elisabeth Joan
authors.initials: EJ
authors.lastname: Barham
authors.forename: Francisco de Assis Carvalho
authors.initials: Fde A
authors.lastname: do Vale
authors.forename: Heloisa Sobreiro
authors.initials: HS
authors.lastname: Selistre-de-Araújo
authors.forename: Márcia Regina
authors.initials: MR
authors.lastname: Cominetti
authors.forename: Sofia Cristina
authors.initials: SC
authors.lastname: Iost Pavarini
mesh_terms: D051722:ADAM Proteins / Q000097:blood*; D000072197:ADAM10 Protein; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology; D000544:Alzheimer Disease / Q000097:blood* / Q000175:diagnosis / Q000453:epidemiology; D053829:Amyloid Precursor Protein Secretases / Q000097:blood*; D015415:Biomarkers / Q000097:blood*; D001792:Blood Platelets / Q000378:metabolism*; D015153:Blotting, Western; D001938:Brazil / Q000453:epidemiology; D003627:Data Interpretation, Statistical; D039721:Diagnostic and Statistical Manual of Mental Disorders; D004522:Educational Status; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000097:blood*; D012727:Sex Characteristics
chemical_list: D015415:Biomarkers; D008565:Membrane Proteins; D053829:Amyloid Precursor Protein Secretases; D051722:ADAM Proteins; D000072197:ADAM10 Protein; C491236:ADAM10 protein, human