title: Quantitative analysis of biofilm formed on vascular prostheses by Staphylococcus epidermidis with different ica and aap genetic status.
abstract: OBJECTIVE
  This study aims to examine biofilm formed on vascular prostheses by Staphylococcus epidermidis with different ica and aap genetic status, and to evaluate the effect of antibiotic-modified prostheses on bacterial colonization.
  
  
  METHODS
  Biofilm formation was determined using fluorescence microscopy imaging. Quantitative analysis was conducted using the biofilm coverage ratio (BCR) calculations.
  
  
  RESULTS
  Our investigations prove that the BCR method with fluorescent dye enabled an accurate assessment of biofilm coverage and comparison of the obtained results. The ica+ aap+ strains formed a biofilm on all of the examined vascular prostheses. Uni-Graft(®) modified with covalently immobilized amikacin was effective in preventing bacterial adherence.
  
  
  CONCLUSIONS
  Molecular biology techniques combined with phenotype studies give a broad insight into biofilm formation mechanisms. On the other hand, fluorescence microscopy imaging along with BCR calculations are reliable and simple tools to quantitatively estimate biofilm formation, as well as the effectiveness of antimicrobial prosthesis modification.
authors.affiliation: Department of Biochemistry and Biotechnology, Medical University in Lublin, Lublin - Poland. joanna.golus@umlub.pl
authors.forename: Joanna
authors.initials: J
authors.lastname: Golus
authors.forename: Magdalena
authors.initials: M
authors.lastname: Stankevic
authors.forename: Rafal
authors.initials: R
authors.lastname: Sawicki
authors.forename: Renata
authors.initials: R
authors.lastname: Los
authors.forename: Anna
authors.initials: A
authors.lastname: Malm
authors.forename: Grazyna
authors.initials: G
authors.lastname: Ginalska
mesh_terms: D000583:Amikacin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics*; D018441:Biofilms / Q000187:drug effects; D001807:Blood Vessel Prosthesis / Q000382:microbiology*; D020099:Coated Materials, Biocompatible; D005838:Genotype; D008856:Microscopy, Fluorescence; D009876:Operon; D010641:Phenotype; D011474:Prosthesis Design; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D020099:Coated Materials, Biocompatible; D000583:Amikacin