title: Mammalian Trit1 is a tRNA([Ser]Sec)-isopentenyl transferase required for full selenoprotein expression.
abstract: Selenoproteins are proteins carrying the rare amino acid Sec (selenocysteine). Full expression of selenoproteins requires modification of tRNA([Ser]Sec), including N(6)-isopentenylation of base A(37). We show that Trit1 is a dimethylallyl:tRNA([Ser]Sec) transferase. Knockdown of Trit1 reduces expression of selenoproteins. Incubation of in vitro transcribed tRNA[Ser]Sec with recombinant Trit1 transfers [(14)C]dimethylallyl pyrophosphate to tRNA([Ser]Sec). 37A>G tRNA([Ser]Sec) is resistant to isopentenylation by Trit1.
authors.affiliation: Institut für Experimentelle Endokrinologie, Charité-Universitätsmedizin Berlin, 13353 Berlin, Germany.
authors.forename: Noelia
authors.initials: N
authors.lastname: Fradejas
authors.forename: Bradley A
authors.initials: BA
authors.lastname: Carlson
authors.forename: Eddy
authors.initials: E
authors.lastname: Rijntjes
authors.forename: Niels-Peter
authors.initials: NP
authors.lastname: Becker
authors.forename: Ryuta
authors.initials: R
authors.lastname: Tobe
authors.forename: Ulrich
authors.initials: U
authors.lastname: Schweizer
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D056945:Hep G2 Cells; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D009690:Nucleic Acid Conformation; D012346:RNA, Transfer, Amino Acyl / Q000235:genetics* / Q000378:metabolism; D051140:Selenoproteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D012346:RNA, Transfer, Amino Acyl; D051140:Selenoproteins; C112705:selenocysteinyl-tRNA; D019883:Alkyl and Aryl Transferases; C579648:tRNA((Ser)Sec)-isopentenyl transferase, mouse