title: Chromosome 11-centric human proteome analysis of human brain hippocampus tissue.
abstract: Human chromosome 11 is the third gene-rich chromosome having 1304 protein-coding genes. According to the GeneCards, this chromosome contains 240 genes related to diseases, as it is well known as a disease-rich chromosome. Although there are many protein-coding genes, the proteomic identification ratio is rather low. As a model study, human hippocampal tissues from patients suffering from Alzheimer's disease and epilepsy were prepared to evaluate the gene-centric statistics related to the gene expression and disorders of chromosome 11. A total of 8828 protein coding genes from brain tissues were extensively off-gel fractionated and profiled by a high resolution mass spectrometer with collision induced dissociation and electron transfer dissociation. Five-hundred twenty-three of the proteins from brain tissues were determined to belong to chromosome 11, representing 37% of the proteins reported in the Global Proteome Machine Database. We extracted gene clusters from a specific biological process or molecular function in gene ontology, among which the olfactory receptor genes showed the largest cluster on chromosome 11. Analysis of the proteome data set from the hippocampus provides a significant network associated with genes and proteins and leads to new insights into the biological and genetic mechanisms of chromosome 11-specific diseases such as Alzheimer's disease.
authors.affiliation: Division of Mass Spectrometry Research, Korea Basic Science Institute, Ochang, Chungbuk, Republic of Korea.
authors.forename: Kyung-Hoon
authors.initials: KH
authors.lastname: Kwon
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Se-Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Hye Kyeong
authors.initials: HK
authors.lastname: Min
authors.forename: Hyoung-Joo
authors.initials: HJ
authors.lastname: Lee
authors.forename: In Jung
authors.initials: IJ
authors.lastname: Ji
authors.forename: Taewook
authors.initials: T
authors.lastname: Kang
authors.forename: Gun Wook
authors.initials: GW
authors.lastname: Park
authors.forename: Hyun Joo
authors.initials: HJ
authors.lastname: An
authors.forename: Bonghee
authors.initials: B
authors.lastname: Lee
authors.forename: Rivka
authors.initials: R
authors.lastname: Ravid
authors.forename: Isidro
authors.initials: I
authors.lastname: Ferrer
authors.forename: Chun Kee
authors.initials: CK
authors.lastname: Chung
authors.forename: Young-Ki
authors.initials: YK
authors.lastname: Paik
authors.forename: William S
authors.initials: WS
authors.lastname: Hancock
authors.forename: Young Mok
authors.initials: YM
authors.lastname: Park
authors.forename: Jong Shin
authors.initials: JS
authors.lastname: Yoo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics / Q000378:metabolism; D030562:Databases, Protein; D004827:Epilepsy / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006624:Hippocampus / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D011506:Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism; D020543:Proteome
chemical_list: D011506:Proteins; D020543:Proteome