title: A novel amperometric biosensor based on ZnO nanoparticles-modified carbon paste electrode for determination of glucose in human serum.
abstract: Zinc oxide nanoparticles-(ZnONPs)modified carbon paste enzyme electrodes (ZnONPsMCPE) were developed for determination of glucose. The determination of glucose was carried out by oxidation of H2O2 at +0.4 V. ZnONPsMCPE provided biocompatible microenvironment for GOx and necessary pathway of electron transfer between GOx and electrode. The response of GOx/ZnONPsMCPE was proportional to glucose concentration and detection limit was 9.1 × 10(-3) mM. Km and Imax, were calculated as 0.124 mM and 2.033 μA. The developed biosensor exhibits high analytical performance with wide linear range (9.1 × 10(-3)-14.5 mM), selectivity and reproducibility. Serum glucose results allow us to ascertain practical utility of GOx/ZnONPsMCPE biosensor.
authors.affiliation: Department of Chemistry, Faculty of Science, Ankara University , Ankara , Turkey.
authors.forename: Gözde
authors.initials: G
authors.lastname: Aydoğdu
authors.forename: Derya Koyuncu
authors.initials: DK
authors.lastname: Zeybek
authors.forename: Şule
authors.initials: Ş
authors.lastname: Pekyardımcı
authors.forename: Esma
authors.initials: E
authors.lastname: Kılıç
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D002244:Carbon / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000378:metabolism; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis; D053768:Metal Nanoparticles / Q000737:chemistry; D010084:Oxidation-Reduction; D044967:Serum / Q000378:metabolism*; D015034:Zinc Oxide / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D002244:Carbon; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose; D015034:Zinc Oxide