title: Comparison of human papillomavirus detection and genotyping with four different prime sets by PCR-sequencing.
abstract: OBJECTIVE
  To assess and compare the Human Papillomavirus (HPV) detection efficiency and the potential clinical utility of PCR sequencing-based technology.
  
  
  METHODS
  Four HPV consensus primer sets (GP5+/6+, MGP, MY09/11, and PGMY09/11) were used in order to amplify a broad spectrum of HPV types for HPV infection in 325 cervical samples and the PCR products were sequenced afterwards for the HPV genotyping.
  
  
  RESULTS
  The HPV-positive rate was 75.4%, of which 35.5% harbored more than one HPV genotype. A total of 36 different genotypes was found, with HPV 16 (24.1%) being the most prevalent, followed by HPV 58 (13.3%) and HPV 52 (9.6%). There were substantial to almost perfect agreements between different primer sets regarding HPV detection efficiency, with the kappa value varying from 0.751 to 0.925, MGP, and PGMY09/11 were the most effective in detecting multiple infections (P < 0.001). With each of the primer sets, a board range of HPV types could be identified, though there were several differences for a few genotypes.
  
  
  CONCLUSIONS
  The substantial agreement between PCR-sequencing and HC2 for the detection of high-risk HPV (kappa=0.761) indicated that PCR-sequencing is also suitable for routine HPV screening.
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology & Immunology, Institute of Microbiology, the Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yu Pin
authors.initials: YP
authors.lastname: Cai
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.forename: Bao Li
authors.initials: BL
authors.lastname: Zhu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Xiao Yu
authors.initials: XY
authors.lastname: Xia
authors.forename: Rui Fen
authors.initials: RF
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiang
mesh_terms: D000328:Adult; D000368:Aged; D052685:Alphapapillomavirus / Q000235:genetics* / Q000302:isolation & purification*; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D055815:Young Adult