title: In vitro protein expression profile of Campylobacter jejuni strain NCTC11168 by two-dimensional gel electrophoresis and mass spectrometry.
abstract: OBJECTIVE
  To investigate the protein expression profiles of the major food-borne pathogen Campylobacter jejuni NCTC11168.
  
  
  METHODS
  Membrane and soluble cellular proteins were extracted from the genome-sequenced C. jejuni strain NCTC11168. Protein expression profiles were determined using two-dimensional gel electrophoresis (2-DE). All the detected spots on the 2-DE map were subjected to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF) analysis.
  
  
  RESULTS
  A total of 537 and 333 spots were detected from the whole cell and membrane-associated proteins of C. jejuni NCTC11168 cultured on Columbia agar medium at 42 °C by 2-DE and Coomassie Brilliant Blue staining, respectively. Analyses of whole cell and membrane-associated proteins included 399 and 133 spots, respectively, which included 182 and 53 functional proteins identified by MALDI-TOF/TOF analysis.
  
  
  CONCLUSIONS
  The comprehensive expression protein profiles of C. jeuni NCTC11168 obtained in this study will be useful for elucidating the roles of these proteins in further pathogenesis investigation.
authors.affiliation: Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. zhangmaojun@icdc.cn
authors.forename: Mao Jun
authors.initials: MJ
authors.lastname: Zhang
authors.forename: Yi Xin
authors.initials: YX
authors.lastname: Gu
authors.forename: Xiao
authors.initials: X
authors.lastname: Di
authors.forename: Fei
authors.initials: F
authors.lastname: Zhao
authors.forename: Yuan Hai
authors.initials: YH
authors.lastname: You
authors.forename: Fan Liang
authors.initials: FL
authors.lastname: Meng
authors.forename: Jian Zhong
authors.initials: JZ
authors.lastname: Zhang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D059467:Transcriptome
chemical_list: D001426:Bacterial Proteins