title: Effect of increasing levels of bioflavonoids in broiler feed on plasma anti-oxidative potential, lipid metabolites, and fatty acid composition of meat.
abstract: This study was conducted to investigate the supplemental effects of purified bioflavonoids (genistein and hesperidin), as potential alternatives to plant/herbs or synthetic antioxidants, individually and in combination for fatty acid profile, lipid metabolites, and antioxidant status of broilers. Three hundred sixty 1-d-old broilers were divided into 6 treatment groups: control (basal diet), G5 (5 mg of genistein per kg of feed), and H20 (20 mg hesperidin per kg of feed), whereas the other 3 groups were supplemented with a mixture of genistein and hesperidin (20% genistein + 80% hesperidin) having a dosage of 5 mg•kg(-1) (GH5), 10 mg•kg(-1) (GH10), and 20 mg•kg(-1) (GH20), respectively. Broilers were slaughtered at 42 d, and breast muscle, liver, and blood samples were collected. A dose-dependent increase (P < 0.05) was observed for plasma antioxidant parameters, including total antioxidant capacity, malondialdehyde production, and total superoxide dismutase activity. Cholesterol and triglyceride contents were found to decrease (P < 0.05) in serum and breast muscle. The proportion of total polyunsaturated fatty acids and the ratio of n-6 to n-3 fatty acids and polyunsaturated fatty acids to saturated fatty acids in breast muscles was significantly improved (P < 0.05) by increasing levels of dietary bioflavonoids. The current results implied that dietary bioflavonoids genistein and hesperidin could positively improve the fatty acid and lipid metabolite profile of broiler breast meat in a dose-dependent fashion. Thus, bioflavonoids could be a feasible alternative of antioxidant plants/herbs and synthetic feed additives for the production of healthier chicken meat.
authors.affiliation: Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: A A
authors.initials: AA
authors.lastname: Kamboh
authors.forename: W-Y
authors.initials: WY
authors.lastname: Zhu
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002645:Chickens; D019587:Dietary Supplements / Q000032:analysis*; D004338:Drug Combinations; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D019833:Genistein / Q000008:administration & dosage*; D006569:Hesperidin / Q000008:administration & dosage*; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood; D008297:Male; D008460:Meat / Q000032:analysis; D010369:Pectoralis Muscles / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D004338:Drug Combinations; D005227:Fatty Acids; D008055:Lipids; D019833:Genistein; D006569:Hesperidin