title: Metabolic profiling of transgenic potato tubers expressing Arabidopsis dehydration response element-binding protein 1A (DREB1A).
abstract: Untargeted metabolome analyses play a critical role in understanding possible metabolic fluctuations of crops under varying environmental conditions. This study reports metabolic profiles of transgenic potato tubers expressing the Arabidopsis DREB1A transcription factor gene, which induces expression of genes involved in environmental stress tolerance. A combination of targeted and untargeted metabolomics demonstrated considerable metabolome differences between the transgenic lines and nontransgenic parent cultivars. In the transgenic lines, stimulation of stress responses was suggested by elevated levels of the glutathione metabolite, γ-aminobutyric acid (GABA), and by the accumulation of β-cyanoalanine, a byproduct of ethylene biosynthesis. These results suggest that the Arabidopsis DREB1A expression might directly or indirectly enhance endogenous potato stress tolerance systems. The results indicate that transgenesis events could alter the metabolic compositions in food crops, and therefore metabolomics analysis could be a most valuable tool to monitor such changes.
authors.affiliation: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai 599-8531, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Iwaki
authors.forename: Lining
authors.initials: L
authors.lastname: Guo
authors.forename: John A
authors.initials: JA
authors.lastname: Ryals
authors.forename: Syuhei
authors.initials: S
authors.lastname: Yasuda
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Shimazaki
authors.forename: Akira
authors.initials: A
authors.lastname: Kikuchi
authors.forename: Kazuo N
authors.initials: KN
authors.lastname: Watanabe
authors.forename: Mie
authors.initials: M
authors.lastname: Kasuga
authors.forename: Kazuko
authors.initials: K
authors.lastname: Yamaguchi-Shinozaki
authors.forename: Takumi
authors.initials: T
authors.lastname: Ogawa
authors.forename: Daisaku
authors.initials: D
authors.lastname: Ohta
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics*; D015870:Gene Expression; D055442:Metabolome; D035281:Plant Tubers / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011198:Solanum tuberosum / Q000235:genetics*; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C114578:DREB1A protein, Arabidopsis; D014157:Transcription Factors; D005680:gamma-Aminobutyric Acid; C004631:3-cyanoalanine; D000409:Alanine