title: Immunoglobulins M survive low-pH conditions used for virus inactivation and for elution from bioaffinity columns.
abstract: Tolerance of low pH is crucial for recombinant proteins to survive conditions that might be experienced during manufacturing such as virus inactivation and elution from bioaffinity columns. In this study, we exposed three different purified immunoglobulins M (IgMs) to pH 3.5 for 60 min at room temperature. Treated samples showed no significant aggregation or fragmentation and retained full immunoreactivity, an intact glycosylation profile, and unchanged thermal stability. Because IgMs are serious candidates for next-generation therapeutics, it is essential to know that some of them are stable at low pH.
authors.affiliation: Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A*STAR), Centros, Singapore 138668, Singapore. monika_mueller@bti.a-star.edu.sg
authors.forename: Monika
authors.initials: M
authors.lastname: Mueller
authors.forename: Corrine
authors.initials: C
authors.lastname: Wan
authors.forename: Kong Meng
authors.initials: KM
authors.lastname: Hoi
authors.forename: Do Yun
authors.initials: DY
authors.lastname: Kim
authors.forename: Hui Theng
authors.initials: HT
authors.lastname: Gan
authors.forename: Muriel
authors.initials: M
authors.lastname: Bardor
authors.forename: Pete
authors.initials: P
authors.lastname: Gagnon
mesh_terms: D002460:Cell Line; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D006031:Glycosylation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007075:Immunoglobulin M / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification; D055550:Protein Stability; D058767:Protein Unfolding; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D013696:Temperature; D038301:Virus Inactivation
chemical_list: D007075:Immunoglobulin M; D011994:Recombinant Proteins