title: Live tissue viability and chemosensitivity assays using digital holographic motility contrast imaging.
abstract: Holographic optical coherence imaging is an en face form of optical coherence tomography that uses low-coherence digital holography as a coherence gate to select light from a chosen depth inside scattering tissue. By acquiring successive holograms at a high camera frame rate at a fixed depth, dynamic speckle provides information concerning dynamic light scattering from intracellular motility. Motility contrast imaging (MCI) uses living motion as a label-free and functional biomarker. MCI provides a new form of viability assay and also is applicable for proliferation and cytotoxicity assays. The results presented here demonstrate that low-coherence digital holography can extract viability information from biologically relevant three-dimensional (3D) tissue based on multicellular tumor spheroids by moving beyond the format of two-dimensional cell culture used for conventional high-content analysis. This paper also demonstrates the use of MCI for chemosensitivity assays on tumor exgrafts of excised ovarian cancer tumors responding to standard-of-care cisplatin chemotherapy. This ex vivo application extends the applicability of MCI beyond 3D tissue culture grown in vitro.
authors.affiliation: Department of Physics, Purdue University, West Lafayette, Indiana 47907, USA. anr@purdue.edu
authors.forename: Ran
authors.initials: R
authors.lastname: An
authors.forename: John
authors.initials: J
authors.lastname: Turek
authors.forename: Daniela Elena
authors.initials: DE
authors.lastname: Matei
authors.forename: David
authors.initials: D
authors.lastname: Nolte
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D001681:Biological Assay / Q000295:instrumentation*; D045744:Cell Line, Tumor; D058948:Cell Tracking / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006696:Holography / Q000295:instrumentation*; D051379:Mice; D008853:Microscopy / Q000295:instrumentation*; D009374:Neoplasms, Experimental / Q000188:drug therapy / Q000473:pathology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*; D014022:Tissue Survival / Q000187:drug effects; D041623:Tomography, Optical Coherence / Q000295:instrumentation*
chemical_list: D000970:Antineoplastic Agents