title: DNA prime-protein boost strategy with replicase-based DNA vaccine against foot-and-mouth disease in bovine calves.
abstract: The limited efficacy of DNA vaccines against foot-and-mouth disease (FMD) in cattle and other natural hosts has prompted a search for a more effective vaccination regimen. In this study we tested a DNA prime-protein boost vaccination strategy against FMD in bovine calves. We used purified recombinant FMDV specific multi-epitope protein (rMEG990) and an optimized sindbis virus replicase-based DNA vaccine expressing this protein (pSinCMV-Vac-MEG990). We demonstrate that vaccination with a low dose of pSinCMV-Vac-MEG990 (10 μg/animal) and subsequently boosting with rMEG990 resulted in induction of neutralizing antibodies, IFN-γ production and protection against homologous virus challenge. However, vaccination with a high dose of pSinCMV-Vac-MEG990 (100 μg/animal) and boosting with rMEG990 resulted in significantly lower immune responses and more severity to the challenge test. Additionally, we show that the post-vaccinal IFN-γ levels in animals correlated positively to their protection against FMDV challenge. These findings suggest that a replicase-based DNA vaccine in proper prime-boost combination may offer an efficient vaccine strategy against FMDV and that IFN-γ could be used as an additional immune parameter to predict protection against FMDV infection.
authors.affiliation: FMD Research Center, Indian Veterinary Research Institute, Banguluru 560024, India.
authors.forename: Pervaiz A
authors.initials: PA
authors.lastname: Dar
authors.forename: Veluvarthy S
authors.initials: VS
authors.lastname: Suryanaryana
authors.forename: G
authors.initials: G
authors.lastname: Nagarajan
authors.forename: Golla R
authors.initials: GR
authors.lastname: Reddy
authors.forename: Hosur J
authors.initials: HJ
authors.lastname: Dechamma
authors.forename: Ganesh
authors.initials: G
authors.lastname: Kondabattula
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D002460:Cell Line; D006224:Cricetinae; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D000939:Epitopes / Q000096:biosynthesis / Q000276:immunology; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000517:prevention & control*; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000276:immunology*; D007371:Interferon-gamma / Q000097:blood; D008297:Male; D014611:Vaccination / Q000592:standards / Q000662:veterinary; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000939:Epitopes; D019444:Vaccines, DNA; D014765:Viral Vaccines; D007371:Interferon-gamma; C023140:DNA replicase; D004259:DNA-Directed DNA Polymerase