title: Polymorphisms within the Promoter Region of the Gamma Interferon (IFN-γ) Receptor1 Gene are Associated with the Susceptibility to Chronic HBV Infection in an Iranian Population.
abstract: BACKGROUND
  chronic hepatitis B virus (HBV) infection is a multifactorial disease that can result in serious clinical complications. Host genetic background especially the genes that encode immunologic factors like INF-γ and its receptor (IFN-γ R) are critical in the pathogenesis of infection.
  
  
  OBJECTIVE
  The current study aimed to investigate the association between two single nucleotide polymorphisms (SNPs) at positions -611 and -56 within the promoter region of gamma interferon receptor1 gene (IFN-γ R1) and chronic HBV infection.
  
  
  METHODS
  Genomic DNA from peripheral blood samples of 200 chronically HBV infected patients and 200 healthy blood donors, as controls, were collected and genomic DNA was extracted by phenol-chloroform method and DNA analysis genotype identification was performed by PCR-RFLP.
  
  
  RESULTS
  The results indicated that both SNP's frequency had a significant difference in the patient and control groups. At position -56, TT genotype was associated with patient group and P value was 0.002 and at position -611, GG genotype was further observed in control group and P value was 0.006.
  
  
  CONCLUSIONS
  Presence of G allele at position -611 within promoter of IFN-γ R1 gene in the enrolled population for the study was related to lower risk of disease, and presence of T allele at position -56 was also related to susceptibility to chronic HBV infection. Men had higher frequency of chronic HBV infection, which might be the result of high risk behavior.
authors.affiliation: Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IR Iran.
authors.forename: Sayyad
authors.initials: S
authors.lastname: Khanizadeh
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Ravanshad
authors.forename: Seyed Reza
authors.initials: SR
authors.lastname: Mohebbi
authors.forename: Hamed
authors.initials: H
authors.lastname: Naghoosi
authors.forename: Mohamad
authors.initials: M
authors.lastname: Abrahim Tahaei
authors.forename: Seyed Dawood
authors.initials: SD
authors.lastname: Mousavi Nasab
authors.forename: Sara
authors.initials: S
authors.lastname: Romani
authors.forename: Pedram
authors.initials: P
authors.lastname: Azimzadeh
authors.forename: Azar
authors.initials: A
authors.lastname: Sanati
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Zali
keywords: Hepatitis B Virus; Interferon Gamma Receptor; Polymorphism, Restriction Fragment Length; Single Nucleotide Polymorphism