title: [Effect of hepatocyte growth factor and transforming growth factor-β(1) on atrial fibroblasts fibrosis].
abstract: OBJECTIVE
  To investigate the effect of hepatocyte growth factor (HGF) and transforming growth factor-β(1) (TGFβ(1)) on the expression of α-smooth muscle actin (α-SMA) and collagen I in human atrial fibroblast in vitro, and to explore the possible molecular mechanism of atrial fibrosis in patients with atrial fibrillation (AF).
  
  
  METHODS
  Human atrial fibroblast, isolated from aseptic right atrial appendage tissues of 10 sinus rhythm (SR) and 10 chronic atrial fibrillation (CAF) patients, were cultured with HGF and TGFβ(1). mRNA expressions of collagen I and α-SMA were detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), the protein expression of α-SMA was determined by immunofluorescence and Western blot.
  
  
  RESULTS
  (1) Compared with SR group, left atrium was significantly dilated in CAF group (t = 2.692, P < 0.05), the mRNA expression of collagen I and α-SMA of atrial fibroblasts were significantly upregulated (all P < 0.01), mRNA expression of collagen I was positively correlated with left atrial dimension (LAD) (r = 0.836, P = 0.014), AF duration (r = 0.739, P = 0.045) and α-SMA mRNA level (r = 0.886, P = 0.012). (2) Compared with SR group, the expression of α-SMA protein in CAF atrial fibroblasts were significantly increased (P < 0.01). (3) TGFβ(1) further stimulated while HGF significantly attenuated the expression of collagen I and α-SMA in CAF atrial fibroblasts (all P < 0.01).
  
  
  CONCLUSIONS
  Increasing expression of collagen I and α-SMA in human atrial fibroblasts might promote atria remodeling leading to the development and sustaining of AF. HGF is involved in the negative regulation on the expression of α-SMA and collagen I.
authors.affiliation: Fujian Provincial Hospital, Fuzhou, China.
authors.forename: Jian-cheng
authors.initials: JC
authors.lastname: Zhang
authors.forename: Jian-quan
authors.initials: JQ
authors.lastname: Chen
authors.forename: Chun-xuan
authors.initials: CX
authors.lastname: Xu
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Ya-zhou
authors.initials: YZ
authors.lastname: Lin
authors.forename: Guo-sheng
authors.initials: GS
authors.lastname: Wu
mesh_terms: D000199:Actins / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D001281:Atrial Fibrillation / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005355:Fibrosis; D015870:Gene Expression; D006325:Heart Atria / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics; D012214:Rheumatic Heart Disease / Q000378:metabolism / Q000473:pathology; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D055815:Young Adult
chemical_list: D000199:Actins; D024042:Collagen Type I; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D017228:Hepatocyte Growth Factor