title: Protective effects of Merlot red wine extract and its major polyphenols in PC12 cells under oxidative stress conditions.
abstract: The potential effect of the extracts from free-run and pressed Merlot red wine has been evaluated in PC12 cells under oxidative stress situation. Comparing both vinification process, pressed Merlot red wine extract possessed higher neuroprotective activity than the free run wine, possibly attributed to the major content in all global polyphenolic families. High performance liquid chromatography determination of individual polyphenols showed that the major compounds found in Merlot red wine extract were quercetin, catechin, epicatechin, tyrosol, gallic acid, and procyanidins. Pretreatments with these polyphenolic compounds (0.25 mM and 0.1 mM, 24 h) significantly increased cell viability of H(2)O(2) and Fenton reaction treated cells. Moreover, these polyphenols attenuated ROS production and decreased the Redox Index of glutathione (RI = GSSG/GSH + GSSG) in cells treated only with Fenton reaction. Furthermore, some polyphenols induced antioxidant enzymes activity and protein expression. Quercetin was the most active. These results support the beneficial effects of red wine extracts and some of its polyphenols under oxidative stress conditions.
  
  
  CONCLUSIONS
  This research provides evidences of the preventive properties of wine extracts and its major polyphenols under oxidative stress conditions.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University Complutense de Madrid, Madrid, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Martín
authors.forename: Elena
authors.initials: E
authors.lastname: González-Burgos
authors.forename: M Emilia
authors.initials: ME
authors.lastname: Carretero
authors.forename: M Pilar
authors.initials: MP
authors.lastname: Gómez-Serranillos
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D044946:Biflavonoids / Q000494:pharmacology; D002392:Catechin / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D005707:Gallic Acid / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000494:pharmacology; D059808:Polyphenols / Q000494:pharmacology*; D044945:Proanthocyanidins / Q000494:pharmacology; D011794:Quercetin / Q000494:pharmacology; D051381:Rats; D014920:Wine / Q000032:analysis*
chemical_list: D000975:Antioxidants; D044946:Biflavonoids; D059808:Polyphenols; D044945:Proanthocyanidins; C011867:4-hydroxyphenylethanol; C017674:procyanidin; D005707:Gallic Acid; D002392:Catechin; D011794:Quercetin; D005978:Glutathione; D010626:Phenylethyl Alcohol