title: Protein profiling of paraquat-exposed rat lungs following treatment             with Acai (Euterpe oleracea Mart.) berry extract.
abstract: Paraquat (1,1'-dimethyl-4,4'-bipyridinium chloride, PQ) is a non-selective             herbicide, and PQ poisoning by accidental or intentional ingestion is a cause             of numerous fatalities around the world every year. Although a great deal of research             has been conducted into the development of an acceptable treatment for PQ poisoning,             no effective guidelines for patients have been developed thus far. Acai berry             extract and juice have been highlighted in this regard, due to their observed             antioxidant effects in various diseases. Furthermore, the acai berry has been             used in dietary supplements, as it contains a variety of nutrients, including             proteins, lipids, vitamins A, C and E and polyphenols. In this study, we conducted             proteomic analysis of PQ-poisoned rat lungs to evaluate the changes in protein             expression induced by PQ and to identify any protective effects of acai berry             on the PQ poisoning. Our data revealed that the expression of the calcium signaling-related             proteins calcium binding protein 1 (CaBP1), FK506 binding protein 4 (FKBP4), S100A6             and secreted protein acidic and rich in cysteine (Sparc, also known as osteonectin)             were induced by PQ treatment and downregulated by acai berry treatment. However,             the levels of protein kinase C substrate 80K-H were shown to be downregulated             as the result of PQ treatment. Our results indicated that these proteins may function             as biomarkers for acute poisoning by PQ exposure. Further studies may be necessary             to understand their clinical relevance with regard to PQ poisoning.
authors.affiliation: Department of Microbiology, College of Medicine, SoonChunHyang University, Cheonan, Chung-Nam 330-090, Republic of Korea.
authors.forename: Yong-Sik
authors.initials: YS
authors.lastname: Kim
authors.forename: Hana
authors.initials: H
authors.lastname: Jung
authors.forename: Tamanna
authors.initials: T
authors.lastname: Zerin
authors.forename: Ho-Yeon
authors.initials: HY
authors.lastname: Song
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D028023:Arecaceae / Q000737:chemistry*; D002135:Calcium-Binding Proteins / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D005638:Fruit / Q000737:chemistry; D005959:Glucosidases / Q000378:metabolism; D006540:Herbicides / Q000633:toxicity*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D008168:Lung / Q000187:drug effects*; D008297:Male; D015676:Osteonectin / Q000378:metabolism; D010269:Paraquat / Q000633:toxicity*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D051381:Rats; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins / Q000378:metabolism; D022021:Tacrolimus Binding Proteins / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins; D006540:Herbicides; D047908:Intracellular Signaling Peptides and Proteins; D015676:Osteonectin; D010936:Plant Extracts; C512948:Prkcsh protein, rat; D020543:Proteome; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; C489132:S100a6 protein, rat; D005959:Glucosidases; D022021:Tacrolimus Binding Proteins; C403485:tacrolimus binding protein 4; C085577:Ca2+-binding protein-1; D010269:Paraquat