title: Warm autoadsorption with enzyme-treated red blood cells.
abstract: Patients demonstrating warm autoantibody specificity present serologic challenges for laboratory staff performing antibody identification in the blood bank. Autoantibody can be removed from plasma or serum by adsorption onto autologous red blood cells (RBCs) provided the patient has not been transfused in the previous 3 months. The adsorption process can be enhanced by enzyme pretreatment of autologous RBCs.
authors.affiliation: Midwest Region American Red Cross Reference Laboratory, American Red Cross, 3838 Dewey Ave., Omaha, NE 68105, USA.
authors.forename: F
authors.initials: F
authors.lastname: Tsimba-Chitsva
authors.forename: S
authors.initials: S
authors.lastname: Bishop
authors.forename: K
authors.initials: K
authors.lastname: Kezeor
mesh_terms: D000327:Adsorption; D000918:Antibody Specificity; D001323:Autoantibodies / Q000276:immunology / Q000302:isolation & purification*; D001771:Blood Banks; D001803:Blood Transfusion; D004912:Erythrocytes / Q000737:chemistry* / Q000166:cytology / Q000187:drug effects; D005361:Ficain / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D010206:Papain / Q000378:metabolism* / Q000494:pharmacology; D013696:Temperature
chemical_list: D001323:Autoantibodies; D010206:Papain; D005361:Ficain