title: Studying microRNAs in lymphoma.
abstract: MicroRNAs (miRNAs) play important roles in development, differentiation, homeostasis, and also in diseases such as lymphoma. This chapter describes methods to study the role of miRNAs in lymphoma. First, we describe a multiplex RT reaction followed by qPCR that can be used to determine differential expression of candidate miRNAs. Second, we provide a protocol for stable overexpression of miRNAs using lentiviral-based ectopic expression systems. Third, we describe a straightforward assay to determine whether the candidate miRNA may function as an oncogene or a tumor suppressor gene in lymphomagenesis.
authors.affiliation: Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands. j.l.kluiver@umcg.nl
authors.forename: Joost
authors.initials: J
authors.lastname: Kluiver
authors.forename: Izabella
authors.initials: I
authors.lastname: Slezak-Prochazka
authors.forename: Anke
authors.initials: A
authors.lastname: van den Berg
mesh_terms: D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D020869:Gene Expression Profiling; D016147:Genes, Tumor Suppressor; D057809:HEK293 Cells; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008223:Lymphoma / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D009857:Oncogenes / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D035683:MicroRNAs