title: Super-resolution microscopy of the neuronal calcium-binding proteins Calneuron-1 and Caldendrin.
abstract: Calcium (Ca(2+)) signaling in neurons is mediated by plethora of calcium binding proteins with many of them belonging to the Calmodulin family of calcium sensors. Many studies have shown that the subcellular localization of neuronal EF-hand Ca(2+)-sensors is crucial for their cellular function. To overcome the resolution limit of classical fluorescence and confocal microscopy various imaging techniques have been developed recently that improve the resolution by an order of magnitude in all dimensions. This new microscope techniques make co-localization studies of Ca(2+)-binding proteins more reliable and help to get insights into the macromolecular organization of intracellular structures and signaling pathways beyond the diffraction limit of visible light.
authors.affiliation: Research Group, Neuroplasticity, Leibniz Institute for Neurobiology (LIN), Magdeburg, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Hradsky
authors.forename: Marina
authors.initials: M
authors.lastname: Mikhaylova
authors.forename: Anna
authors.initials: A
authors.lastname: Karpova
authors.forename: Michael R
authors.initials: MR
authors.lastname: Kreutz
authors.forename: Werner
authors.initials: W
authors.lastname: Zuschratter
mesh_terms: D000818:Animals; D019556:COS Cells; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D018929:Cell Culture Techniques; D002522:Chlorocebus aethiops; D006056:Golgi Apparatus / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D007425:Intracellular Membranes / Q000378:metabolism; D008055:Lipids / Q000737:chemistry; D008853:Microscopy / Q000379:methods*; D009474:Neurons / Q000378:metabolism*; D021381:Protein Transport; D051381:Rats; D014162:Transfection
chemical_list: D002135:Calcium-Binding Proteins; D008055:Lipids; C086724:Lipofectamine; C516555:calneuron I, rat; C085577:Ca2+-binding protein-1