title: Flavonoids from Malus hupehensis and their cardioprotective effects against doxorubicin-induced toxicity in H9c2 cells.
abstract: Three biflavonoid glycosides along with 12 known flavonoids, were isolated from leaves of Malus hupehensis. The complete structures of two of the compounds were established from analysis of MS, NMR spectroscopic and CD data, as well as DFT CD calculations they were determined to be atropisomeric along a central biaryl axis. The antioxidant activities and protective effects of the compounds against doxorubicin-induced cardiomyopathy in H9c2 cells were also investigated. Amongst all of the isolated compounds, quercetin was the most active radical scavenger with EC(50) values of 3.2 μM and 17.8 μM by the DPPH and ABTS(+) methods, respectively. The results indicated that three of the flavanoids also had a strong protective influence against doxorubicin-induced cell death with EC(50) values of 8.3, 5.2 and 7.6 μM, respectively.
authors.affiliation: Department of Natural Product Chemistry, Key Laboratory of Chemical Biology of Ministry of Education, Shandong University, Jinan 250012, PR China.
authors.forename: Shu-Qi
authors.initials: SQ
authors.lastname: Wang
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Zhu
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Shen
authors.forename: Feng
authors.initials: F
authors.lastname: Xiang
authors.forename: Hong-Xiang
authors.initials: HX
authors.lastname: Lou
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D044946:Biflavonoids / Q000737:chemistry* / Q000494:pharmacology*; D002460:Cell Line; D004317:Doxorubicin / Q000633:toxicity*; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry* / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D027845:Malus / Q000737:chemistry*; D013058:Mass Spectrometry; D011794:Quercetin / Q000737:chemistry / Q000494:pharmacology; D051381:Rats
chemical_list: D044946:Biflavonoids; D005419:Flavonoids; D006027:Glycosides; C041388:avicularin; D004317:Doxorubicin; D011794:Quercetin