title: MEF10 is required for RNA editing at nad2-842 in mitochondria of Arabidopsis thaliana and interacts with MORF8.
abstract: A forwards genetic screen of a chemically mutated plant population identified mitochondrial RNA editing factor 10 (MEF10) in Arabidopsis thaliana. MEF10 is a trans-factor required specifically for the C to U editing of site nad2-842. The MEF10 protein is characterized by a stretch of pentatricopeptide repeats (PPR) and a C-terminal extension domain ending with the amino acids DYW. Editing is lost in mutant plants but is recovered by transgenic introduction of an intact MEF10 gene. The MEF10 protein interacts with multiple organellar RNA editing factor 8 (MORF8) but not with other mitochondrial MORF proteins in yeast two hybrid assays. These results support the model that specific combinations of MORF and MEF proteins are involved in RNA editing in plant mitochondria.
authors.affiliation: Molekulare Botanik, Universität Ulm, 89069, Ulm, Germany. barbara.haertel@uni-ulm.de
authors.forename: Barbara
authors.initials: B
authors.lastname: Härtel
authors.forename: Anja
authors.initials: A
authors.lastname: Zehrmann
authors.forename: Daniil
authors.initials: D
authors.lastname: Verbitskiy
authors.forename: Johannes A
authors.initials: JA
authors.lastname: van der Merwe
authors.forename: Axel
authors.initials: A
authors.lastname: Brennicke
authors.forename: Mizuki
authors.initials: M
authors.lastname: Takenaka
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D017124:Conserved Sequence; D060146:Ecotype; D005020:Ethyl Methanesulfonate; D017343:Genes, Plant / Q000235:genetics; D008928:Mitochondria / Q000235:genetics*; D024101:Mitochondrial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D011485:Protein Binding / Q000235:genetics; D017434:Protein Structure, Tertiary; D017393:RNA Editing / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D020798:Two-Hybrid System Techniques; D014529:Uridine / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C579841:MEF10 protein, Arabidopsis; C579840:MORF8 protein, Arabidopsis; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D005020:Ethyl Methanesulfonate; D014529:Uridine