title: Cerebrospinal fluid proteome comparison between multiple sclerosis patients and controls.
abstract: OBJECTIVE
  The aim of the present study was to identify proteins in cerebrospinal fluid (CSF) with different abundance between patients with relapsing-remitting multiple sclerosis (RRMS) and controls. Such proteins may be diagnostic biomarkers and contribute with novel information about the disease pathogenesis.
  
  
  METHODS
  Cerebrospinal fluid from patients with RRMS (n = 17) and controls (n = 17) were trypsin digested and analyzed in a label-free fashion using liquid chromatography mass spectrometry. The resulting data were analyzed using SearchGUI, PeptideShaker, and the Progenesis software.
  
  
  RESULTS
  Two hundred and ninety-one proteins were identified, of which 32 were significantly differentially abundant between the patients with RRMS and controls (P-value ≤ 0.05, two or more peptides quantified). Among these were proteins which previously have been linked to MS, including immunoglobulin subunits, vitamin D-binding protein, apolipoprotein D, kallikrein-6, neuronal pentraxin receptor, Dickkopf-related protein 3, and contactin-1.
  
  
  CONCLUSIONS
  The study provides an overview of differentially abundant proteins between RRMS and controls, and a few of these are further discussed. It should be stressed that a larger verification study is needed to reveal the potential value of these proteins as biomarkers for RRMS and their involvement in the disease pathogenesis.
authors.affiliation: The KG Jebsen Centre for MS-research, Department of Clinical Medicine, University of Bergen, Bergen, Norway. ann.kroksveen@med.uib.no
authors.forename: A C
authors.initials: AC
authors.lastname: Kroksveen
authors.forename: A
authors.initials: A
authors.lastname: Guldbrandsen
authors.forename: C
authors.initials: C
authors.lastname: Vedeler
authors.forename: K M
authors.initials: KM
authors.lastname: Myhr
authors.forename: J A
authors.initials: JA
authors.lastname: Opsahl
authors.forename: F S
authors.initials: FS
authors.lastname: Berven
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000134:cerebrospinal fluid; D002556:Cerebrospinal Fluid Proteins / Q000134:cerebrospinal fluid*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020528:Multiple Sclerosis, Chronic Progressive / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D014809:Vitamin D-Binding Protein / Q000134:cerebrospinal fluid
chemical_list: D015415:Biomarkers; D002556:Cerebrospinal Fluid Proteins; D020543:Proteome; D014809:Vitamin D-Binding Protein