title: Translational control of JunB, an AP-1 transcription factor, in activated human endothelial cells.
abstract: Stimulated endothelial cells (EC) assume an activated phenotype with pro-inflammatory and prothrombotic features, requiring new gene and protein expression. New protein synthesis in activated EC is largely regulated by transcriptional events controlled by a variety of transcription factors. However, post-transcriptional control of gene expression also influences phenotype and allows the cell to alter protein expression in a faster and more direct way than is typically possible with transcriptional mechanisms. We sought to demonstrate that post-transcriptional control of gene expression occurs during EC activation. Using thrombin-activated EC and a high-throughput, microarray-based approach, we identified a number of gene products that may be regulated through post-transcriptional mechanisms, including the AP-1 transcription factor JunB. Using polysome profiling, cytoplasts and other standard cell biologic techniques, JunB is shown to be regulated at a post-transcriptional level during EC activation. In activated EC, the AP-1 transcription factor JunB, is regulated on a post-transcriptional level. Signal-dependent control of translation may regulate transcription factor expression and therefore, subsequent transcriptional events in stimulated EC.
authors.affiliation: Division of Vascular Surgery, University of Utah Health Sciences Center, Salt Lake City, Utah 84132, USA.
authors.forename: Douglas I
authors.initials: DI
authors.lastname: Schmid
authors.forename: Hansjörg
authors.initials: H
authors.lastname: Schwertz
authors.forename: Huimiao
authors.initials: H
authors.lastname: Jiang
authors.forename: Robert A
authors.initials: RA
authors.lastname: Campbell
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Weyrich
authors.forename: Thomas M
authors.initials: TM
authors.lastname: McIntyre
authors.forename: Guy A
authors.initials: GA
authors.lastname: Zimmerman
authors.forename: Larry W
authors.initials: LW
authors.lastname: Kraiss
mesh_terms: D001483:Base Sequence; D005786:Gene Expression Regulation / Q000235:genetics / Q000502:physiology; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D011499:Protein Processing, Post-Translational / Q000235:genetics / Q000502:physiology; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C581727:JunB protein, human; D018808:Transcription Factor AP-1; D014157:Transcription Factors