title: Acetylation of endogenous STAT proteins.
abstract: Acetylation of signal transducer and activator of transcription (STAT) proteins has been recognized as a significant mechanism for the regulation of their cellular functions. Site-specific antibodies are available only for a minority of STATs. The detection of acetylated STATs by immunoprecipitation (IP) followed by western blot (WB) will be described in the following chapter. Defined conditions for cell lysis and IP will be elucidated on the basis of STAT1 acetylation.
authors.affiliation: Center for Molecular Biomedicine (CMB), Department of Biochemistry, University of Jena, Jena, Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: Ginter
authors.forename: Thorsten
authors.initials: T
authors.lastname: Heinzel
authors.forename: Oliver H
authors.initials: OH
authors.lastname: Krämer
mesh_terms: D000107:Acetylation; D000818:Animals; D015153:Blotting, Western / Q000379:methods*; D002457:Cell Extracts; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D008239:Lysine / Q000378:metabolism; D051379:Mice; D050791:STAT Transcription Factors / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D050794:STAT1 Transcription Factor / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D002457:Cell Extracts; D050791:STAT Transcription Factors; D050794:STAT1 Transcription Factor; D008239:Lysine