title: Innovative vaccination protocol against vibriosis in Dicentrarchus labrax (L.) juveniles: improvement of immune parameters and protection to challenge.
abstract: The effect of vaccination on immune parameters of European sea bass, Dicentrarchus labrax, is not fully established, as well as surveyed throughout rearing till the commercial size. Furthermore, available information on the possible role of booster treatments is scarce. Sea bass juveniles were vaccinated against Listonella anguillarum using a commercial bivalent formulation administered by immersion (priming: 95 dph; booster: 165 dph) or by immersion (priming: 95 dph; booster: 165 dph) and subsequent i.p. injection (booster: 233 dph). Serum specific IgM and numbers of IgM(+) cells in head kidney and spleen evidenced B-cell responses mainly after the immersion booster, accompanied by increased TcR-β transcripts and leucocyte respiratory burst. Immune enhancement was confirmed by the protection towards i.p. challenges with a virulent strain. RPS accounted for >70% in fish immersion-boosted and near 100% in fish further boosted i.p. Differently from usual farm practices, this innovative vaccination protocol proved to be highly effective. Booster treatments are therefore strongly recommended.
authors.affiliation: Department of Food Sciences, Section of Veterinary Pathology, University of Udine, Italy. marco.galeotti@uniud.it
authors.forename: Marco
authors.initials: M
authors.lastname: Galeotti
authors.forename: Nicla
authors.initials: N
authors.lastname: Romano
authors.forename: Donatella
authors.initials: D
authors.lastname: Volpatti
authors.forename: Chiara
authors.initials: C
authors.lastname: Bulfon
authors.forename: Andrea
authors.initials: A
authors.lastname: Brunetti
authors.forename: Pietro Giorgio
authors.initials: PG
authors.lastname: Tiscar
authors.forename: Francesco
authors.initials: F
authors.lastname: Mosca
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Bertoni
authors.forename: Maria Gabriella
authors.initials: MG
authors.lastname: Marchetti
authors.forename: Luigi
authors.initials: L
authors.lastname: Abelli
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D001492:Bass / Q000276:immunology; D005393:Fish Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D060226:Head Kidney / Q000276:immunology; D007101:Immersion; D007075:Immunoglobulin M / Q000097:blood; D007274:Injections, Intraperitoneal; D053526:Listonella / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D013154:Spleen / Q000276:immunology; D014611:Vaccination / Q000379:methods*
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D007075:Immunoglobulin M