title: Methods of dopamine research in retina cells.
abstract: Dopamine is the main catecholamine found in the retina of most species, being synthesized from the L-amino acid tyrosine. Its effects are mediated by G protein coupled receptors subfamilies that are commonly coupled to adenylyl cyclase in opposite manners. There is evidence that this amine works as a developmental signal in the embryonic retina and several distinct roles have been attributed to dopamine in the retina such as proliferation, synaptogenesis, neuroprotection, increased signal transmission in cone, gap junction modulation, neuronal-pigmented epithelium-glial communication, and neuron-glia interaction. Here we describe methods that have been used in the study of the dopaminergic function in the retina in the last 40 years. We emphasize the approaches used in the studies on the development of the avian and rodent retina. The dopaminergic system is one of the first phenotypes to appear in the developing vertebrate retina.
authors.affiliation: Department of Neurobiology, Program in Neurosciences, Universidade Federal Fluminense, Niterói, Brazil.
authors.forename: Ana Lucia Marques
authors.initials: AL
authors.lastname: Ventura
authors.forename: Fernando Garcia
authors.initials: FG
authors.lastname: de Mello
authors.forename: Ricardo Augusto
authors.initials: RA
authors.lastname: de Melo Reis
mesh_terms: D000818:Animals; D001552:Benzazepines / Q000494:pharmacology; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D018929:Cell Culture Techniques; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002645:Chickens; D000242:Cyclic AMP / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D007150:Immunohistochemistry; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011954:Receptors, Dopamine / Q000378:metabolism; D012160:Retina / Q000166:cytology* / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013569:Synapses / Q000378:metabolism; D046509:Tissue Culture Techniques
chemical_list: D001552:Benzazepines; D015415:Biomarkers; D012333:RNA, Messenger; D011954:Receptors, Dopamine; C534628:SCH 23390; D000242:Cyclic AMP; D004298:Dopamine