title: In vitro genotoxicity and cytotoxicity in murine fibroblasts exposed to EDTA, NaOCl, MTAD and citric acid.
abstract: The aim of the present study was to evaluate the capacity of some root canal irrigants to induce genetic damage and/or cellular death in vitro. Murine fibroblast cells were exposed to ethylenediaminetetraacetic acid (EDTA), sodium hypochlorite (NaOCl), MTAD™ and citric acid in increasing concentrations for 3 h at 37ºC. The negative control group was treated with vehicle control (phosphate buffer solution - PBS) for 3 h at 37°C, and the positive control group was treated with methylmetanesulfonate, 1 μM. for 3 h at 37°C. Cytotoxicity was assessed by the trypan blue test and genotoxicity was evaluated by the single cell gel (comet) assay. The results showed that exposure to 2.5% and 5% NaOCl and 8.5% citric acid resulted in a significant cytotoxic effect. NaOCl, EDTA and citric acid did not produce genotoxic effects with respect to the comet assay data for all evaluated concentrations. Although MTAD was not a cytotoxic agent, it showed significant genotoxic effects at all tested concentrations (ANOVA and Tukey's test; p<0.05). NaOCl, EDTA and citric acid were found to be cytotoxic in a dose-dependent manner, but they were not genotoxic. MTAD did not cause cell death, but presented genotoxic effects.
authors.affiliation: Proclin Department, Dental School, State University of Rio de Janeiro, Brazil.
authors.forename: Juliana Soares Roter
authors.initials: JS
authors.lastname: Marins
authors.forename: Luciana Moura
authors.initials: LM
authors.lastname: Sassone
authors.forename: Sandra Rivera
authors.initials: SR
authors.lastname: Fidel
authors.forename: Daniel Araki
authors.initials: DA
authors.lastname: Ribeiro
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D016923:Cell Death / Q000187:drug effects*; D002460:Cell Line; D019343:Citric Acid / Q000633:toxicity; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D003804:Dentin / Q000187:drug effects*; D004318:Doxycycline / Q000633:toxicity; D004492:Edetic Acid / Q000633:toxicity; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D009153:Mutagens; D011136:Polysorbates / Q000633:toxicity; D012388:Root Canal Irrigants / Q000633:toxicity*; D012973:Sodium Hypochlorite / Q000633:toxicity; D014343:Trypan Blue / Q000737:chemistry
chemical_list: C475043:MTAD (intracanal irrigant); D009153:Mutagens; D011136:Polysorbates; D012388:Root Canal Irrigants; D019343:Citric Acid; D004492:Edetic Acid; D012973:Sodium Hypochlorite; D014343:Trypan Blue; D004318:Doxycycline