title: Age-related changes in the global DNA methylation profile of leukocytes are linked to nutrition but are not associated with the MTHFR C677T genotype or to functional capacities.
abstract: Global DNA methylation of peripheral blood leukocytes has been recently proposed as a potential biomarker for disease risk. However, the amplitude of the changes in DNA methylation associated with normal aging and the impacts of environmental changes on this variation are still unclear. In this context, we evaluated the association of global DNA methylation with nutritional habits, tobacco smoking, body mass index (BMI), clinical laboratory parameters, polymorphism C677T MTHFR, functional cognition and the daily practice of physical activity in a cancer-free older population. Leukocyte global DNA methylation from 126 older individuals was quantified using a high-throughput ELISA-based method. Global DNA hypomethylation was observed in older individuals when compared to a younger population (p = 0.0469), confirming changes in DNA methylation in the aging process. Furthermore, the methylation profile of elders was correlated with the daily ingestion of carbohydrates (p = 0.0494), lipids (p = 0.0494), vitamin B6 (p = 0.0421), magnesium (p = 0.0302), and also to the serum levels of total protein (p = 0.0004), alpha 2 globulin (p = 0.0013) and albumin (p = 0.0015). No statistically significant difference was observed when global DNA methylation were stratified according to C677T MTHFR genotypes (p = 0.7200), BMI (p = 0.1170), smoking habit (p = 0.4382), physical activity in daily life (p = 0.8492), scored cognitive function (p = 0.7229) or depression state (p = 0.8301). Our data indicate that age-related variations in the global DNA methylation profile of leukocytes might be modulated by the daily intake of carbohydrates, lipids, vitamin B6, and magnesium and be associated with serum protein levels, however it is independent of C677T MTHFR genotype and not correlated with BMI, smoking habit, cognitive function or the routine physical activities.
authors.affiliation: Research Centre on Health Sciences, University of Northern Parana (UNOPAR), Londrina, Paraná, Brazil. mvmgomes@gmail.com
authors.forename: Marcus V M
authors.initials: MV
authors.lastname: Gomes
authors.forename: Leandro V
authors.initials: LV
authors.lastname: Toffoli
authors.forename: Douglas W
authors.initials: DW
authors.lastname: Arruda
authors.forename: Larissa M
authors.initials: LM
authors.lastname: Soldera
authors.forename: Gislaine G
authors.initials: GG
authors.lastname: Pelosi
authors.forename: Rejane D
authors.initials: RD
authors.lastname: Neves-Souza
authors.forename: Eliane R
authors.initials: ER
authors.lastname: Freitas
authors.forename: Denilson T
authors.initials: DT
authors.lastname: Castro
authors.forename: Audrey S
authors.initials: AS
authors.lastname: Marquez
mesh_terms: D000368:Aged; D000375:Aging / Q000235:genetics*; D015992:Body Mass Index; D019175:DNA Methylation / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D007962:Leukocytes / Q000201:enzymology*; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D009747:Nutritional Physiological Phenomena / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012907:Smoking / Q000235:genetics
chemical_list: C573423:MTHFR protein, human; D042965:Methylenetetrahydrofolate Reductase (NADPH2)