title: Synthesis, characterization of penicillin G capped silver nanoconjugates to combat β-lactamase resistance in infectious microorganism.
abstract: In the present study, a novel strategy was adopted to synthesize, β lactamase resistant penicillin G molecules by using the unique properties of silver nanoparticles. Ascorbic acid-stabilized spherical monodispersed silver nanoparticles were prepared by a simple chemical reaction. The formation processes of the silver nanoparticles were investigated by UV-vis spectroscopy and Atomic Force Microscopy (AFM). Free amine groups were introduced on the surface of native silver nanoparticles by coating a uniform layer of polyaniline and this was confirmed by FTIR spectroscopy and Scanning Electron Microscopy. Functionalized silver nanoparticles were then grafted to the C3 carboxyl group of the β lactam ring of penicillin G in the presence of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDAC) with a conjugation yield of 213 μg mg⁻¹. These novel silver penicillin G nanoconjugates showed a very good growth inhibition against both non-resistant Escherichia coli (gram negative) as well as toward β lactam resistant, E. coli (gram negative) and Staphylococcus aureus (gram positive).
authors.affiliation: Department of Biochemistry, Maharshi Dayanand University, Rohtak 124001, Haryana, India.
authors.forename: Vasim
authors.initials: V
authors.lastname: Ahmed
authors.forename: Jitendra
authors.initials: J
authors.lastname: Kumar
authors.forename: Manoj
authors.initials: M
authors.lastname: Kumar
authors.forename: Manu Bhambi
authors.initials: MB
authors.lastname: Chauhan
authors.forename: Manika
authors.initials: M
authors.lastname: Vij
authors.forename: Munia
authors.initials: M
authors.lastname: Ganguli
authors.forename: Nar Singh
authors.initials: NS
authors.lastname: Chauhan
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D001709:Biotechnology / Q000379:methods*; D004926:Escherichia coli / Q000187:drug effects*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D058726:Nanoconjugates / Q000737:chemistry*; D010400:Penicillin G / Q000737:chemistry* / Q000494:pharmacology*; D012834:Silver / Q000737:chemistry / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects*; D014961:X-Ray Diffraction; D018440:beta-Lactam Resistance / Q000187:drug effects*; D001618:beta-Lactamases / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D058726:Nanoconjugates; D012834:Silver; D001618:beta-Lactamases; D010400:Penicillin G