title: Gravitropic response induced by coumarin: evidences of ROS distribution involvement.
abstract: Coumarin effects on gravitropic responses of Arabidopsis thaliana roots were here evaluated. Coumarin alone did not cause any alteration on gravitropic response showing a behavior similar to control plants. In contrast, TIBA and NPA, two auxin transport inhibitors, strongly modified root gravitropic responses. The addition of coumarin to the medium together with TIBA or NPA partially restored the effect of both inhibitors. Simultaneously, a semi-quantitative evaluation of ROS distribution was performed on root tips. TIBA and NPA caused a wide distribution of O 2(-), ROS oxidant species, around the root tip which disappeared with coumarin addition to both treatments, restoring ROS localized distribution. These results indicated a strong correlation between ROS distribution and coumarin-mediated recovery of root gravitropism.
authors.affiliation: Dipartimento di Biotecnologie per il Monitoraggio Agro-Alimentare ed Ambientale (BIOMAA); Università Mediterranea di Reggio Calabria; Salita Melissari; Reggio Calabria, RC Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Lupini
authors.affiliation: Dipartimento di Biotecnologie per il Monitoraggio Agro-Alimentare ed Ambientale (BIOMAA); Università Mediterranea di Reggio Calabria; Salita Melissari; Reggio Calabria, RC Italy.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Araniti
authors.affiliation: Dipartimento di Biotecnologie per il Monitoraggio Agro-Alimentare ed Ambientale (BIOMAA); Università Mediterranea di Reggio Calabria; Salita Melissari; Reggio Calabria, RC Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Sunseri
authors.affiliation: Dipartimento di Biotecnologie per il Monitoraggio Agro-Alimentare ed Ambientale (BIOMAA); Università Mediterranea di Reggio Calabria; Salita Melissari; Reggio Calabria, RC Italy.
authors.forename: Maria Rosa
authors.initials: MR
authors.lastname: Abenavoli
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000378:metabolism; D003374:Coumarins / Q000494:pharmacology*; D018522:Gravitropism / Q000187:drug effects / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014283:Triiodobenzoic Acids / Q000494:pharmacology
chemical_list: D029681:Arabidopsis Proteins; D003374:Coumarins; D017382:Reactive Oxygen Species; D014283:Triiodobenzoic Acids; C030123:coumarin; C052968:2,3,5-triiodobenzoic acid
keywords: Arabidopsis thaliana; coumarin; gravitropism response; reactive oxygen species