title: Proteomic methods reveal cyclophilin A function as a host restriction factor against rotavirus infection.
abstract: Rotavirus (RV) infection is the main cause of acute dehydrating diarrhea in infants and young children below 5 years old worldwide. RV infection causes a global shutoff of host proteins as many other viruses do. However, previous studies revealed that RV could selectively upregulated the expression of some host proteins that then played important roles in RV infection. To globally explor such host proteins that were upregulated in early human rotavirus (HRV) infection, proteomic methods were used and a total of ten upregulated host proteins were unambiguously identified. Cyclophilin A (CYPA), a peptidyl-prolyl cis-trans isomerase, was among these upregulated host proteins. Following infection, CYPA was recruited to the viroplasm and interacted with HRV structural protein VP2; CYPA reduced host susceptibility to HRV infection and inhibited replication of HRV by repressing the expression of viral proteins. Furthermore, we found that the increased expression of CYPA in enterocytes of small intestine correlated to the period when BALB/c mice became resistant to RV diarrhea. Together, we identified CYPA as a novel host restriction factor that confered protection against RV infection and might contribute to host susceptibility to RV diarrhea.
authors.affiliation: Institute of Immunology, Third Military Medical University, Chongqing, P. R. China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: He
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Mou
authors.forename: Wanling
authors.initials: W
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Fei
authors.forename: Yan
authors.initials: Y
authors.lastname: Tang
authors.forename: Ji
authors.initials: J
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Yan
authors.forename: Zhengqiong
authors.initials: Z
authors.lastname: Chen
authors.forename: Xia
authors.initials: X
authors.lastname: Yang
authors.forename: Zigang
authors.initials: Z
authors.lastname: Shen
authors.forename: Jintao
authors.initials: J
authors.lastname: Li
authors.forename: Yuzhang
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D036022:Capsid Proteins / Q000378:metabolism; D021984:Cyclophilin A / Q000378:metabolism*; D003967:Diarrhea / Q000378:metabolism / Q000473:pathology / Q000821:virology; D060467:Disease Resistance; D004198:Disease Susceptibility; D020895:Enterocytes / Q000378:metabolism / Q000473:pathology / Q000821:virology; D054884:Host-Pathogen Interactions / Q000276:immunology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D012401:Rotavirus / Q000502:physiology*; D012400:Rotavirus Infections / Q000276:immunology / Q000378:metabolism* / Q000821:virology*; D015854:Up-Regulation; D014779:Virus Replication
chemical_list: D036022:Capsid Proteins; C442545:VP2 protein, Rotavirus; D021984:Cyclophilin A