title: Synthesis of galactothiophosphoesters of uridine and preliminary tests to evaluate their activity against selected glycosyltransferases.
abstract: Glycoconjugates (8-13) were obtained in condensation reaction of selectively protected thiophosphoesters of uridine with 2,3,4.6-tetra-O-acetyl-1-thio-beta-D-galactose. In sequence of reactions: selective protection of esculine, glycosylation reaction and deprotecion beta-D-galactopiranoside-(1-4)-esculine (20) was obtained as a model of the product reaction catalyzed by GTs. Prepared glycococnjugates (8-13) were checked using TLC methods in terms of inhibition of bovine milk beta-1,4-galactosyltransferase.
authors.affiliation: Silesian University of Technology, Department of Chemistry, Gliwice, Poland. marta.grec@polsl.pl
authors.forename: Marta
authors.initials: M
authors.lastname: Grec
authors.forename: Piotr
authors.initials: P
authors.lastname: Swierk
authors.forename: Gabriela
authors.initials: G
authors.lastname: Pastuch-Gawołek
authors.forename: Wiesław
authors.initials: W
authors.lastname: Szeja
mesh_terms: D000818:Animals; D002417:Cattle; D004791:Enzyme Inhibitors / Q000138:chemical synthesis*; D006001:Glycoconjugates / Q000138:chemical synthesis* / Q000494:pharmacology; D016695:Glycosyltransferases / Q000037:antagonists & inhibitors*; D008892:Milk / Q000201:enzymology; D014529:Uridine / Q000737:chemistry
chemical_list: D004791:Enzyme Inhibitors; D006001:Glycoconjugates; D016695:Glycosyltransferases; D014529:Uridine