title: Longistatin, an EF-hand Ca2+-binding protein from vector tick: identification, purification, and characterization.
abstract: EF-hand Ca(2+)-binding motif, a structural component of the EF-hand protein, functions as a calcium sensor and/or buffer in the cytosol of the cell. However, in a few exceptional cases, the EF-hand proteins are secreted from cells and play crucial roles extracellularly. We have identified longistatin, an EF-hand Ca(2+)-binding protein, from the salivary glands of the tick, Haemaphysalis longicornis. Longistatin possesses an N-terminal sequence of unknown structure and two EF-hand motifs in the C-terminus, which conserve a calmodulin-like canonical structure. Longistatin shows distinct changes in its migration during electrophoresis through SDS-PAGE gel containing calcium or ethylenediaminetetraacetic acid (EDTA). Both recombinant and endogenous forms of longistatin can be stained with rutheninum red, demonstrating that longistatin is a Ca(2+)-binding protein.
authors.affiliation: Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.lastname: Anisuzzaman
authors.forename: M Khyrul
authors.initials: MK
authors.lastname: Islam
authors.forename: M Abdul
authors.initials: MA
authors.lastname: Alim
authors.forename: Naotoshi
authors.initials: N
authors.lastname: Tsuji
mesh_terms: D000818:Animals; D001096:Arachnid Vectors; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D003956:Dialysis; D020832:EF Hand Motifs; D024202:Electrophoretic Mobility Shift Assay; D026863:Ixodidae; D011994:Recombinant Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D012430:Ruthenium Red / Q000378:metabolism; D012469:Salivary Glands / Q000166:cytology; D012471:Salivary Proteins and Peptides / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D013194:Staining and Labeling
chemical_list: D002135:Calcium-Binding Proteins; D011994:Recombinant Proteins; D012471:Salivary Proteins and Peptides; C559189:longistatin protein, Haemaphysalis longicornis; D012430:Ruthenium Red