title: Evaluation of two decellularization methods in the development of a whole-organ decellularized rat liver scaffold.
abstract: OBJECTIVE
  Hepatic tissue engineering is considered as a possible alternative to liver transplantation for end-stage liver disease. Several methods of decellularization of xenogeneic liver are available to produce three-dimensional organ scaffolds for engineering liver tissues. However, rare studies have examined and compared the effectiveness of different methods on the structure and composition of intact decellularized liver extracellular matrix.
  
  
  METHODS
  Two decellularization methods were adopted herein. Their effects on collagen, elastin, glycosaminoglycans (GAGs), hepatocyte growth factor (HGF) content and influence to the function of hepatocytes cultured in scaffolds were examined and compared.
  
  
  RESULTS
  The complete tissue decellularization was successfully achieved after treatment with sodium dodecyl sulphate (SDS) and Triton X-100. The total absence of nuclear structures and removal of viable cells were confirmed by haematoxylin-eosin staining and scanning electron microscopy. Collagen was preserved after both treatments. However, the elastin content decreased to about 20% and 60%, the GAGs content decreased to about 10% and 50% and the HGF content decreased to about 20% and 60% of the native liver level after SDS and Triton X-100 treatment respectively. The Triton X-100-treated scaffolds were much superior than SDS-treated scaffolds in supporting liver-specific function, including albumin secretion (P = 0.001), urea synthesis (P = 0.002), ammonia elimination (P = 0.007) and mRNA expression levels of drug metabolism enzymes.
  
  
  CONCLUSIONS
  This study suggested that liver extracellular matrix scaffolds constructed using perfusion of Triton X-100 as described herein might provide a more effective and ideal material for the usage in tissue engineering and regenerative medicine approaches.
authors.affiliation: Department of Hepatobiliary Surgery, the Affiliated DrumTower Hospital of Nanjing University Medical School, Nanjing, China.
authors.forename: Haozhen
authors.initials: H
authors.lastname: Ren
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Shi
authors.forename: Liang
authors.initials: L
authors.lastname: Tao
authors.forename: Jiangqiang
authors.initials: J
authors.lastname: Xiao
authors.forename: Bing
authors.initials: B
authors.lastname: Han
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xianwen
authors.initials: X
authors.lastname: Yuan
authors.forename: Yitao
authors.initials: Y
authors.lastname: Ding
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism; D004549:Elastin / Q000378:metabolism; D058625:End Stage Liver Disease / Q000628:therapy*; D005109:Extracellular Matrix / Q000502:physiology*; D006025:Glycosaminoglycans / Q000378:metabolism; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism; D008099:Liver / Q000166:cytology*; D017830:Octoxynol; D051381:Rats; D044968:Regenerative Medicine / Q000379:methods*; D012967:Sodium Dodecyl Sulfate; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D006025:Glycosaminoglycans; D012967:Sodium Dodecyl Sulfate; D017228:Hepatocyte Growth Factor; D017830:Octoxynol; D003094:Collagen; D004549:Elastin