title: Cloning and characterization of a novel bifunctional acetyl xylan esterase with carbohydrate binding module from Phanerochaete chrysosporium.
abstract: The cDNA of acetyl xylan esterase 2 (PcAxe2) gene containing a carbohydrate binding module (CBM) sequence from Phanerochaete chrysosporium was cloned and expressed in Pichia pastoris. The recombinant PcAxe2 protein (rPcAxe2) was efficiently produced, reaching a maximum of 1058 U l(-1) after 6 days of cultivation. Molecular mass of the rPcAxe2 on SDS-PAGE was approximately 63 kDa under hyperglycosylation. Optimal activity of the purified rPcAxe2 enzyme was observed at pH and temperature of 7.0 and 30-35°C, respectively. In addition to acetyl xylan esterase activity, rPcAxe2 also exhibited a xylanase activity at an optimum pH and temperature of 5.0 and 80°C, respectively. The synergistic action of rPcAxe2 with rPcXynC on birchwood xylan, beechwood xylan and wheat arabinoxylan enhanced the total reducing soluble sugar.
authors.affiliation: Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, 194-5 Madong, Iksan, Jeonbuk 570-752, Republic of Korea.
authors.forename: Nguyen Duc
authors.initials: ND
authors.lastname: Huy
authors.forename: Saravanakumar
authors.initials: S
authors.lastname: Thiyagarajan
authors.forename: Dae-Hyuk
authors.initials: DH
authors.lastname: Kim
authors.forename: Seung-Moon
authors.initials: SM
authors.lastname: Park
mesh_terms: D000115:Acetylesterase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D002482:Cellulose / Q000378:metabolism; D003001:Cloning, Molecular; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D008969:Molecular Sequence Data; D010463:Peracetic Acid / Q000378:metabolism; D020075:Phanerochaete / Q000201:enzymology*; D010843:Pichia / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D013696:Temperature; D014990:Xylans / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D014990:Xylans; D002482:Cellulose; D000115:Acetylesterase; C081851:acetylxylan esterase; D043364:Endo-1,4-beta Xylanases; D010463:Peracetic Acid