title: Degradable terpolymers with alkyl side chains demonstrate enhanced gene delivery potency and nanoparticle stability.
abstract: Degradable, cationic poly(β-amino ester)s (PBAEs) with alkyl side chains are developed for non-viral gene delivery. Nanoparticles formed from these PBAE terpolymers exhibit significantly enhanced DNA transfection potency and resistance to aggregation. These hydrophobic PBAE terpolymers, but not PBAEs lacking alkyl side chains, support interaction with PEG-lipid conjugates, facilitating their functionalization with shielding and targeting moieties and accelerating the in vivo translation of these materials.
authors.affiliation: Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Eltoukhy
authors.forename: Delai
authors.initials: D
authors.lastname: Chen
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Alabi
authors.forename: Robert
authors.initials: R
authors.lastname: Langer
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Anderson
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008055:Lipids / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D011092:Polyethylene Glycols / Q000737:chemistry / Q000633:toxicity; D011108:Polymers / Q000737:chemistry* / Q000633:toxicity; D014162:Transfection
chemical_list: D008055:Lipids; D011108:Polymers; C507253:poly(beta-amino ester); D011092:Polyethylene Glycols; D004247:DNA