title: Determination of captopril by high-performance liquid chromatography with direct electrogenerated chemiluminescence.
abstract: Captopril exhibit electrogenerated chemiluminescence (ECL) in NaNO(3) solution when constant current is exerted. Based on this observation, a direct ECL method coupled with high-performance liquid chromatography (HPLC) separation is developed for determination of captopril in human serum. Factors affected the ECL emission are investigated. Under the optimal conditions, the ECL intensity has a linear relationship with the concentration of captopril in the range of 4.0×10(-6)-2.0×10(-3) g mL(-1) and the detection limit is 2×10(-6) g mL(-1) (S/N=3). Compared with the common electrogenerated chemiluminescence experiments, the developed method need no any other fluorescence additives.
authors.affiliation: Chengdu University of Technology, Chengdu 610059, People's Republic of China. yonghua_sun@126.com
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Sun
authors.forename: Zhujun
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xinfeng
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000097:blood*; D000959:Antihypertensive Agents / Q000097:blood*; D002216:Captopril / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D004867:Equipment Design; D006801:Humans; D057230:Limit of Detection; D049449:Luminescence; D008163:Luminescent Measurements / Q000295:instrumentation
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D002216:Captopril