title: Performance evaluation of the Abbott CELL-DYN Emerald for use as a bench-top analyzer in a research setting.
abstract: BACKGROUND
  The CELL-DYN Emerald is a compact bench-top hematology analyzer that can be used for a three-part white cell differential analysis. To determine its utility for analysis of human and mouse samples, we evaluated this machine against the larger CELL-DYN Sapphire and Sysmex XT2000iV hematology analyzers.
  
  
  METHODS
  120 human (normal and abnormal) and 30 mouse (normal and abnormal) samples were analyzed on both the CELL-DYN Emerald and CELL-DYN Sapphire or Sysmex XT2000iV analyzers. For mouse samples, the CELL-DYN Emerald analyzer required manual recalibration based on the histogram populations.
  
  
  RESULTS
  Analysis of the CELL-DYN Emerald showed excellent precision, within accepted ranges (white cell count CV% = 2.09%; hemoglobin CV% = 1.68%; platelets CV% = 4.13%). Linearity was excellent (R² ≥ 0.99), carryover was minimal (<1%), and overall interinstrument agreement was acceptable for both human and mouse samples. Comparison between the CELL-DYN Emerald and Sapphire analyzers for human samples or Sysmex XT2000iV analyzer for mouse samples showed excellent correlation for all parameters.
  
  
  CONCLUSIONS
  The CELL-DYN Emerald was generally comparable to the larger reference analyzer for both human and mouse samples. It would be suitable for use in satellite research laboratories or as a backup system in larger laboratories.
authors.affiliation: Gene and Stem Cell Therapy Program, Centenary Institute, Camperdown, NSW, Australia.
authors.forename: T-L
authors.initials: TL
authors.lastname: Khoo
authors.forename: N
authors.initials: N
authors.lastname: Xiros
authors.forename: F
authors.initials: F
authors.lastname: Guan
authors.forename: D
authors.initials: D
authors.lastname: Orellana
authors.forename: J
authors.initials: J
authors.lastname: Holst
authors.forename: D E
authors.initials: DE
authors.lastname: Joshua
authors.forename: J E J
authors.initials: JE
authors.lastname: Rasko
mesh_terms: D000818:Animals; D057205:Automation, Laboratory / Q000295:instrumentation / Q000592:standards*; D001772:Blood Cell Count; D006405:Hematology / Q000295:instrumentation / Q000592:standards*; D006801:Humans; D007753:Laboratories / Q000592:standards*; D051379:Mice; D012016:Reference Values; D015203:Reproducibility of Results
keywords: Analytical performance; CELL-DYN Emerald; automated hematology analyzer; human full blood count; mouse full blood count