title: Common amino acid sequences deduced from coding exons of the porcine FGF4 gene in two breeds and production of the encoded protein in Escherichia coli.
abstract: Fibroblast growth factor 4 (FGF4) is considered a crucial gene in the development of mammalian embryos. Here we identified common amino acid sequences predicted from coding exons of the FGF4 gene in five pigs of two breeds, and HispFGF4, a 6× histidine-tagged porcine FGF4, was produced in Escherichia coli. HispFGF4 was purified efficiently from the supernatant of cell lysate by heparin column chromatography. In a porcine embryonic fibroblast cell line, HispFGF4 showed significant mitogenic activities at concentrations as low as 0.001 nM (p<0.01). To the best of our knowledge, this is the first report describing the complete nucleotide sequence of coding exons for the porcine FGF4 protein in two breeds, together with the production of a recombinant, bioactive porcine FGF4 derivative.
authors.affiliation: Graduate School of Bioresource Sciences, Akita Prefectural University, Japan.
authors.forename: Saiko
authors.initials: S
authors.lastname: Sugawara
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Ito
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Suzuki
authors.forename: Toshikiyo
authors.initials: T
authors.lastname: Takahashi
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Konishi
authors.forename: Masaki
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Sho
authors.initials: S
authors.lastname: Sato
authors.forename: Yuki
authors.initials: Y
authors.lastname: Mori
authors.forename: Kano
authors.initials: K
authors.lastname: Kasuga
authors.forename: Ikuo
authors.initials: I
authors.lastname: Kojima
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Fukuda
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kobayashi
mesh_terms: D000818:Animals; D001483:Base Sequence; D001947:Breeding; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005091:Exons; D051519:Fibroblast Growth Factor 4 / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D016415:Sequence Alignment; D013552:Swine / Q000196:embryology / Q000235:genetics*
chemical_list: D051519:Fibroblast Growth Factor 4; D011993:Recombinant Fusion Proteins