title: [Effects of oil-refining microbes (genus Acinetobacter) on cytogenetical structures of human lymphocytes in cell cultures].
abstract: The objective of this study was to assess ability of oil-refining bacteria Acinetobacter calcoaceticus and A. valentis to induce karyopathological abnormalities and chromosomal aberrations in human lymphocyte cultures. It was found that the cultures infected with A. calcoaceticus showed significantly high frequencies of cytogenetical effects and chromosomal aberrant cells as compared to the intact cultures and cultures infected with A. valentis. The most of chromosomal aberrations, mainly chromatid aberrations, were located in 1 and 2 chromosomes. Moreover, the aberrations were detected in some specific chromosome areas. Abnormalities of mitotic cell division and nucleus morphology were determined in lymphocyte cultures infected with A. calcoaceticus. There were found significantly high frequencies of cells with micronuclei, nucleus protrusions, anaphase or metaphase chromosome and chromosomal fragments lagging as well as multipolar and C-mitoses. Thus, the oil-refining bacteria A. calcoaceticus in contrast to A. valentis demonstrated strong genotoxic effects in human lymphocyte cultures in vitro.
authors.forename: N N
authors.initials: NN
authors.lastname: Il'inskikh
authors.forename: E N
authors.initials: EN
authors.lastname: Il'inskikh
authors.forename: I N
authors.initials: IN
authors.lastname: Il'inskikh
mesh_terms: D000150:Acinetobacter / Q000254:growth & development / Q000472:pathogenicity*; D002467:Cell Nucleus / Q000235:genetics / Q000382:microbiology / Q000648:ultrastructure*; D053144:Cell Nucleus Shape; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000382:microbiology / Q000473:pathology; D008938:Mitosis; D010578:Petroleum
chemical_list: D010578:Petroleum