title: The global regulator Crc plays a multifaceted role in modulation of type III secretion system in Pseudomonas aeruginosa.
abstract: The opportunistic pathogen Pseudomonas aeruginosa utilizes type III secretion system (T3SS) to translocate effector proteins into eukaryotic host cells that subvert normal host cell functions to the benefit of the pathogen, and results in serious infections. T3SS in P. aeruginosa is controlled by a complex system of regulatory mechanisms and signaling pathways. In this study, we described that Crc, an RNA-binding protein, exerts a positive impact on T3SS in P. aeruginosa, as evidenced by promoter activity assays of several key T3SS genes, transcriptomics, RT-PCR, and immunoblotting in crc mutant. We further demonstrated that the regulatory function of Crc on the T3SS was mediated through the T3SS master regulator ExsA and linked to the Cbr/Crc signaling system. Expression profiling of the crc mutant revealed a downregulation of flagship T3SS genes as well as 16 other genes known to regulate T3SS gene expression in P. aeruginosa. On the basis of these data, we proposed that Crc may exert multifaceted control on the T3SS through various pathways, which may serve to fine-tune this virulence mechanism in response to environmental changes and nutrient sources.
authors.affiliation: Institute of Molecular and Cell Biology, 61 Biopolis Drive, Singapore, 138673. dongyh@imcb.a-star.edu.sg
authors.forename: Yi-Hu
authors.initials: YH
authors.lastname: Dong
authors.forename: Xi-Fen
authors.initials: XF
authors.lastname: Zhang
authors.forename: Lian-Hui
authors.initials: LH
authors.lastname: Zhang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D058947:Bacterial Secretion Systems; D015964:Gene Expression Regulation, Bacterial; D055786:Gene Knockout Techniques; D015151:Immunoblotting; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D059467:Transcriptome
chemical_list: D001426:Bacterial Proteins; D058947:Bacterial Secretion Systems; D012097:Repressor Proteins; C101896:crc protein, Pseudomonas