title: Transforming growth factor-β1 deteriorates microrheological characteristics and motility of mature dendritic cells in concentration-dependent fashion.
abstract: Dendritic cells (DCs) are potent and specialized antigen-presenting cells that play a crucial role in initiating and amplifying both the innate and adaptive immune responses. Tumor cells can escape from immune attack by secreting suppressive cytokines which solely or cooperatively impair the immune function and microrheological properties of DCs. However, the underlying mechanisms are not fully defined. Transforming growth factor-β1 (TGF-β1) has been identified as a major cytokine in the tumor microenvironment. To determine the effects of TGF-β1 on mature DCs (mDCs) from microrheological viewpoint, cells were treated with different concentrations of TGF-β1. The results showed that the impaired microrheological parameters, including osmotic fragility, electrophoretic mobility, deformability, membrane fluidity, F-actin organization and so on, as well as motilities of mDCs relied heavily on TGF-β1 concentration. Moreover, these changes were correlated with the expression levels of fascin1, cofilin1, phosphorylated cofilin1 and profilin, this could be one of the crucial aspects of immune escape mechanisms of tumors, hinting that the signal pathway of TGF-β1 should be blocked in appropriate way before performing DCs-based immunotherapy against cancer. It is clinically important to understand the biological behavior of DCs and immune escape mechanism of tumor as well as how to improve efficiency of the anti-tumor therapy based on DCs.
authors.affiliation: Department of Biotechnology, Guiyang Medical College, Guiyang, Guizhou Province, China Department of Cell Biology, Guiyang Medical College, Guiyang, Guizhou Province, China.
authors.forename: Qinni
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: Department of Head and Neck, Affiliated Hospital and Cancer Hospital, Guiyang Medical College, Guiyang, Guizhou Province, China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Long
authors.affiliation: Department of Medical Physics, Chengde Medical College, Chengde, Hebei Province, China.
authors.forename: Binbin
authors.initials: B
authors.lastname: Jia
authors.affiliation: Department of Biotechnology, Guiyang Medical College, Guiyang, Guizhou Province, China Department of Cell Biology, Guiyang Medical College, Guiyang, Guizhou Province, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Cell Biology, Guiyang Medical College, Guiyang, Guizhou Province, China.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Nephrology, Affiliated Hospital of Guiyang Medical College, Guiyang, Guizhou Province, China.
authors.forename: Long
authors.initials: L
authors.lastname: Li
authors.affiliation: Hemorheology Center, School of Basic Medical Sciences, Health Science Center of Peking University, Beijing, China.
authors.forename: Zongyao
authors.initials: Z
authors.lastname: Wen
authors.affiliation: Department of Head and Neck, Affiliated Hospital and Cancer Hospital, Guiyang Medical College, Guiyang, Guizhou Province, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Jin
authors.affiliation: Hemorheology Center, School of Basic Medical Sciences, Health Science Center of Peking University, Beijing, China.
authors.forename: Weijuan
authors.initials: W
authors.lastname: Yao
authors.affiliation: Department of Biotechnology, Guiyang Medical College, Guiyang, Guizhou Province, China Department of Cell Biology, Guiyang Medical College, Guiyang, Guizhou Province, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Zeng
mesh_terms: D000199:Actins / Q000378:metabolism; D015703:Antigens, CD / Q000097:blood; D017209:Apoptosis / Q000187:drug effects; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D016014:Linear Models; D018613:Microscopy, Confocal; D009996:Osmotic Fragility; D012212:Rheology / Q000379:methods; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D055682:Viscoelastic Substances / Q000737:chemistry
chemical_list: D000199:Actins; D015703:Antigens, CD; D053773:Transforming Growth Factor beta1; D055682:Viscoelastic Substances
keywords: Mature dendritic cells; immune escape mechanism of tumor; microrheological characteristics; transforming growth factor-$\beta_{1}$