title: Abundance and diversity of copper resistance genes cusA and copA in microbial communities in relation to the impact of copper on Chilean marine sediments.
abstract: Microorganisms have developed copper-resistance mechanisms in order to survive in contaminated environments. The abundance of the copper-resistance genes cusA and copA, encoding respectively for a Resistance Cell Nodulation protein and for a P-type ATP-ase pump, was assessed in copper and non-copper-impacted Chilean marine sediment cores by the use of molecular tools. We demonstrated that number of copA and cusA genes per bacterial cell was higher in the contaminated sediment, and that copA gene was more abundant than cusA gene in the impacted sediment. The molecular phylogeny of the two copper-resistance genes was studied and reveals an impact of copper on the genetic composition of copA and cusA genes.
authors.affiliation: Faculté des Sciences, Université de Rouen, CNRS UMR 6143-M2C, Groupe de Microbiologie, Place Emile Blondel, 76821 Mont Saint Aignan Cedex, France. besaury.ludovic@gmail.com
authors.forename: Ludovic
authors.initials: L
authors.lastname: Besaury
authors.forename: Josselin
authors.initials: J
authors.lastname: Bodilis
authors.forename: Fabien
authors.initials: F
authors.lastname: Delgas
authors.forename: Santiago
authors.initials: S
authors.lastname: Andrade
authors.forename: Rodrigo
authors.initials: R
authors.lastname: De la Iglesia
authors.forename: Baghdad
authors.initials: B
authors.lastname: Ouddane
authors.forename: Laurent
authors.initials: L
authors.lastname: Quillet
mesh_terms: D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics*; D001483:Base Sequence; D002677:Chile; D003300:Copper / Q000032:analysis / Q000633:toxicity*; D004784:Environmental Monitoring / Q000379:methods; D005798:Genes, Bacterial; D014644:Genetic Variation; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology*; D026901:Membrane Transport Proteins / Q000032:analysis / Q000235:genetics*; D008969:Molecular Sequence Data; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D001426:Bacterial Proteins; C071263:CopA protein, Bacteria; D026901:Membrane Transport Proteins; D014874:Water Pollutants, Chemical; D003300:Copper