title: Long term and standard incubations of WST-1 reagent reflect the same inhibitory trend of cell viability in rat airway smooth muscle cells.
abstract: The WST-1 assay is an efficient test for cell viability measurement and the standard incubation time is 2h. In order to test if one-time addition of WST-1 reagent can reflect the relative cell viability trend of the testing agents at different time points, the effects of 2h standard incubation time and long term incubation time (2h+24h, 2h+48h) of WST-1 were compared in the rat airway smooth muscle cells (ASM cells) after adding of the testing protein MRP-14. Our study demonstrated that the effect of different dosages of the protein after 2h WST-1 incubation on ASM cells showed a tendency of inhibition and achieved the maximal inhibition effect at 72h. The relative cell viability trend of the 2h+24h group was the same to that of the 2h WST-1 incubation, which means that 24h prolonged incubation time of WST-1 reagent could still reflect the relative cell viability trend. In conclusion, the study suggested that the WST-1 is a proper candidate reagent for continuous monitation of cell viability.
authors.affiliation: Laboratory of Molecular Biology, Shanghai Research Institute of Acupuncture and Meridian, Yue Yang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Lei-Miao
authors.initials: LM
authors.lastname: Yin
authors.forename: Yin
authors.initials: Y
authors.lastname: Wei
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Yu-Dong
authors.initials: YD
authors.lastname: Xu
authors.forename: Yong-Qing
authors.initials: YQ
authors.lastname: Yang
mesh_terms: D000818:Animals; D040502:Calgranulin B / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects* / Q000502:physiology; D008168:Lung / Q000166:cytology / Q000187:drug effects; D032389:Myocytes, Smooth Muscle / Q000187:drug effects*; D051381:Rats; D013778:Tetrazolium Salts / Q000008:administration & dosage*
chemical_list: C110656:2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium; D040502:Calgranulin B; D013778:Tetrazolium Salts
keywords: Cell viability; Rat airway smooth muscle cells.; WST-1