title: [Cloning and bioinformatic analysis of FatB genes in Lonicera japonica Thunb and its substitutes].
abstract: A FatB unigene was obtained from the transcriptome dataset of Lonicera japonica Thunb. Full-length FatB cDNA was cloned from buds of Lonicera japonica Thunb., Lonicera japonica Thunb. var. chinensis (Wats.) Bak., Lonicera hypoglauca Miq. and Lonicera dasystyla Rehd. using RT-PCR technology, and named as LJFatB, LHFatB, LJCFatB and LDFatB. The results of bioinformatic analysis showed that LJFatB, LJCFatB, LHFatB and LDFatB and Arabidopsis thaliana AtFatB had a closely relationship. Nucleotide sequences and protein secondary structure of LJFatB, LJCFatB, LHFatB and LDFatB are different and their proteins had conserved FatB substrate binding sites and catalytic activity sites. Transcriptive level of LJFatB, LJCFatB, LHFatB and LDFatB in bud was not significantly different. Therefore, LJFatB, LJCFatB, LHFatB and LDFatB could have the same biological function as AtFatB.
authors.affiliation: Wuhan Polytechnic University, Wuhan, China.
authors.forename: Zhou-yong
authors.initials: ZY
authors.lastname: Wang
authors.forename: Chao
authors.initials: C
authors.lastname: Jiang
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Shu-fang
authors.initials: SF
authors.lastname: Lin
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics; D035264:Flowers / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D031239:Lonicera / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000378:metabolism; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017433:Protein Structure, Secondary; D013869:Thiolester Hydrolases / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D013869:Thiolester Hydrolases; C018748:oleoyl-(acyl-carrier-protein) hydrolase