title: Photoheterotrophic growth of Chlorella vulgaris ESP6 on organic acids from dark hydrogen fermentation effluents.
abstract: In this work, Chlorella vulgaris ESP6 was used to assimilate the soluble metabolites in the hydrogen fermentation broth of Clostridium butyricum CGS5 to obtain valuable microalgae biomass. The results show that C. vulgaris ESP6 could grow on the 4-fold diluted dark fermentation broth. Acetate was efficiently utilized during the growth of C. vulgaris ESP6, whereas the microalgae growth was inhibited by lactate, butyrate, and HCO3(-) when their concentrations were higher than 0.5, 0.1, and 2.72 g/L, respectively. C. vulgaris ESP6 could completely consume butyrate (the most abundant dark fermentation metabolite) when it was grown on Tris-Acetate-Phosphate medium under a food to microorganism (F/M) ratio of 1.11.
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan, ROC.
authors.forename: Chien-Hung
authors.initials: CH
authors.lastname: Liu
authors.forename: Chin-Yen
authors.initials: CY
authors.lastname: Chang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liao
authors.forename: Xun
authors.initials: X
authors.lastname: Zhu
authors.forename: Jo-Shu
authors.initials: JS
authors.lastname: Chang
mesh_terms: D000085:Acetates / Q000378:metabolism; D001639:Bicarbonates / Q000378:metabolism; D057919:Biological Oxygen Demand Analysis; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002087:Butyrates / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism*; D048408:Chlorella vulgaris / Q000254:growth & development*; D046972:Clostridium butyricum / Q000378:metabolism*; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism*; D007700:Kinetics; D019344:Lactic Acid / Q000378:metabolism
chemical_list: D000085:Acetates; D001639:Bicarbonates; D002087:Butyrates; D002245:Carbon Dioxide; D019344:Lactic Acid; D006859:Hydrogen
keywords: Chlorella vulgaris; Clostridium butyricum; Dark fermentation; Food to microorganism ratio; Volatile fatty acids