title: Aggregation of the salivary proline-rich protein IB5 in the presence of the tannin EgCG.
abstract: In the mouth, proline-rich proteins (PRP), which are major components of stimulated saliva, interact with tannins contained in food. We report in vitro interactions of the tannin epigallocatechin gallate (EgCG), with a basic salivary PRP, IB5, studied through electrospray ionization mass spectrometry (ESI-MS), small-angle X-ray scattering (SAXS), and dynamic light scattering (DLS). In dilute protein (IB5) solutions of low ionic strength (1 mM), the proteins repel each other, and the tannins bind to nonaggregated proteins. ESI-MS experiments determine the populations of nonaggregated proteins that have bound various numbers of tannin molecules. These populations match approximately the Poisson distribution for binding to n = 8 sites on the protein. MS/MS experiments confirm that complexes containing n = 1 to 8 EgCG molecules are dissociated with the same energy. Assuming that the 8 sites are equivalent, we calculate a binding isotherm, with a binding free energy Δμ = 7.26RT(a) (K(d) = 706 μM). In protein solutions that are more concentrated (0.21 mM) and at higher ionic strength (50 mM, pH 5.5), the tannins can bridge the proteins together. DLS experiments measure the number of proteins per aggregate. This number rises rapidly when the EgCG concentration exceeds a threshold (0.2 mM EgCG for 0.21 mM of IB5). SAXS experiments indicate that the aggregates have a core-corona structure. The core contains proteins that have bound at least 3 tannins and the corona has proteins with fewer bound tannins. These aggregates coexist with nonaggregated proteins. Increasing the tannin concentration beyond the threshold causes the transfer of proteins to the aggregates and a fast rise of the number of proteins per aggregate. A poisoned growth model explains this fast rise. Very large cationic aggregates, containing up to 10,000 proteins, are formed at tannin concentrations (2 mM) slightly above the aggregation threshold (0.2 mM).
authors.affiliation: DISCO beamline, Synchrotron Soleil, l'Orme des Merisiers, 91192 Gif sur Yvette, France.
authors.forename: Francis
authors.initials: F
authors.lastname: Canon
authors.forename: Franck
authors.initials: F
authors.lastname: Paté
authors.forename: Véronique
authors.initials: V
authors.lastname: Cheynier
authors.forename: Pascale
authors.initials: P
authors.lastname: Sarni-Manchado
authors.forename: Alexandre
authors.initials: A
authors.lastname: Giuliani
authors.forename: Javier
authors.initials: J
authors.lastname: Pérez
authors.forename: Dominique
authors.initials: D
authors.lastname: Durand
authors.forename: Joaquim
authors.initials: J
authors.lastname: Li
authors.forename: Bernard
authors.initials: B
authors.lastname: Cabane
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002392:Catechin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D055503:Protein Multimerization / Q000187:drug effects*; D020836:Protein Structure, Quaternary; D055273:Salivary Proline-Rich Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D055273:Salivary Proline-Rich Proteins; D002392:Catechin; C045651:epigallocatechin gallate