title: Quantitative one-step RT-PCR assay for rapid and sensitive identification and titration of polioviruses in clinical specimens.
abstract: Rapid identification and quantitation of polioviruses in clinical specimens is important for surveillance and analysis of virus shedding by vaccine recipients, which could be used to assess the level of mucosal immunity. A quantitative one step RT-PCR was developed for identification and titration of all three poliovirus serotypes. The assay could be an alternative to the traditional procedure based on cell culture isolation and subsequent determination of poliovirus serotype and virus titration. The method is based on quantitative PCR performed with reverse transcription reaction in the same tube. The multiplex assay that quantifies all three serotypes of poliovirus was found to be highly specific, sensitive, and takes only one day to complete.
authors.affiliation: Center for Biologics Evaluation and Research, US Food and Drug Administration, 1401 Rockville Pike, HFM 470, Rockville, MD 20852-1448, USA. majid.laassri@fda.hhs.gov
authors.forename: Majid
authors.initials: M
authors.lastname: Laassri
authors.forename: Anthony
authors.initials: A
authors.lastname: Dipiazza
authors.forename: Bella
authors.initials: B
authors.lastname: Bidzhieva
authors.forename: Tatiana
authors.initials: T
authors.lastname: Zagorodnyaya
authors.forename: Konstantin
authors.initials: K
authors.lastname: Chumakov
mesh_terms: D002460:Cell Line; D005243:Feces / Q000821:virology; D006801:Humans; D011051:Poliomyelitis / Q000175:diagnosis*; D017955:Poliovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D011055:Poliovirus Vaccine, Oral; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017201:Virus Shedding
chemical_list: D011055:Poliovirus Vaccine, Oral; D012367:RNA, Viral