title: Interaction of Salmonella typhimurium with dendritic cells derived from pluripotent embryonic stem cells.
abstract: Using an in vitro differentiation protocol we isolated cells with the properties of dendritic cells (DCs) from immunologically refractive pluripotent murine embryonic stem cells (ESCs). These ES-derived dendritic cells (ESDCs) expressed cytokines and were able to present antigen to a T cell line. Infection of ESDCs with Salmonella Typhimurium stimulated the expression of immune cell markers and thousands of murine genes, many associated with the immune response. Consequently, this system provides a novel in vitro model, amenable to genetic modification, for monitoring host/pathogen interactions.
authors.affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, United Kingdom.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Rossi
authors.forename: Christine
authors.initials: C
authors.lastname: Hale
authors.forename: David
authors.initials: D
authors.lastname: Goulding
authors.forename: Robert
authors.initials: R
authors.lastname: Andrews
authors.forename: Zarah
authors.initials: Z
authors.lastname: Abdellah
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Fairchild
authors.forename: Gordon
authors.initials: G
authors.lastname: Dougan
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D053595:Embryonic Stem Cells / Q000166:cytology*; D005434:Flow Cytometry; D051379:Mice; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D012486:Salmonella typhimurium / Q000276:immunology* / Q000502:physiology*