title: Development of novel molecularly imprinted magnetic solid-phase extraction materials based on magnetic carbon nanotubes and their application for the determination of gatifloxacin in serum samples coupled with high performance liquid chromatography.
abstract: A novel composite imprinted material, on the basis of magnetic carbon nanotubes (MCNTs)-incorporated layer using gatifloxacin as a template, methacrylic acid as a functional monomer, and ethylene glycol dimethacrylate as a cross-linker, was successfully synthesized by a surface imprinting technique. Adsorption dynamics and a Scatchard adsorption model were employed to evaluate the adsorption process. The results showed that magnetic carbon nanotubes molecularly imprinted polymers (MCNTs@MIP) displayed a rapid dynamic adsorption and a high adsorption capacity of 192.7 μg/mg toward GTFX. Applied MCNTs@MIP as a sorbent, a magnetic solid phase extraction method coupled with high performance liquid chromatography (MSPE-HPLC) was developed for the determination of GTFX in serum samples. The recoveries from 79.1±4.8% to 85.3±4.2% were obtained. MCNTs@MIP can not only be collected and separated fast by external magnetic field but also have high surface-to-volume ratio, outstanding mechanical properties and specific recognition toward template molecule. In addition, the MCNTs@MIP could be regenerated, which could be used for five cycles with lost of less than 7.8% of its recovery on average. These analytical results of serum samples display that the proposed method based on MCNTs@MIP is applicable for fast and selective extraction of therapeutic agents from biological fluids.
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Deli
authors.initials: D
authors.lastname: Xiao
authors.forename: Pierre
authors.initials: P
authors.lastname: Dramou
authors.forename: Nanqian
authors.initials: N
authors.lastname: Xiong
authors.forename: Hua
authors.initials: H
authors.lastname: He
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Danhua
authors.initials: D
authors.lastname: Yuan
authors.forename: Hao
authors.initials: H
authors.lastname: Dai
mesh_terms: D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000097:blood / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D024841:Fluoroquinolones / Q000097:blood / Q000302:isolation & purification*; D000077734:Gatifloxacin; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008689:Methacrylates / Q000737:chemistry; D008958:Models, Molecular; D054802:Molecular Imprinting / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D058185:Magnetite Nanoparticles; D008689:Methacrylates; D037742:Nanotubes, Carbon; C008384:methacrylic acid; C004919:ethylene dimethacrylate; D000077734:Gatifloxacin