title: Population subdivision of Japanese flounder Paralichthys olivaceus in the Pacific Coast of Tohoku Japan detected by means of mitochondrial phylogenetic information.
abstract: This study deals with mitochondrial phylogenetic information of Japanese flounder in the Pacific coast of Tohoku Japan to estimate the genetic population subdivision that was undetectable by conventional population statistics. We determined complete sequences of mitochondrial NADH dehydrogenase subunit-2 (ND2) and  subunit-5 (ND5) genes for 151 individuals from northern (Aomori and Iwate prefectures, 40-41°N) and southern (Miyagi and Fukushima prefectures, 37-38°N) waters. Samples from both waters showed high genetic diversity, including 126 haplotypes. These haplotypes were located at mixed and nested positions on an inferred phylogenetic tree, and traditional F-statistics indicated no significant population divergence (φ(ST) = -0.00335,  p > 0.05), corroborating our previous study. Three variable sites, however, showed significant base composition heterogeneity between samples from the northern and southern waters (Fisher's exact-test, p < 0.01). Nucleotide substitutions at the three sites converged on an apical clade, which consisted of the five southern individuals, whereas its sister clade consisted only of the three northern individuals. This phylogenetic information corroborates previous ecological studies indicating the presence of separate stocks in the northern and southern waters.
authors.affiliation: Research Center for Fisheries Oceanography and Marine Ecosystem, National Research Institute of Fisheries Science, Fisheries Research Agency,  Fukuura, Kanazawa, Yokohama 236-8648, Japan. yshig@affrc.go.jp.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Shigenobu
authors.forename: Michio
authors.initials: M
authors.lastname: Yoneda
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kurita
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ambe
authors.forename: Kenji
authors.initials: K
authors.lastname: Saitoh
mesh_terms: D000818:Animals; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D029941:Fish Proteins / Q000235:genetics*; D005432:Flounder / Q000235:genetics*; D014644:Genetic Variation; D005828:Genetics, Population; D005843:Geography; D006239:Haplotypes; D007564:Japan; D009245:NADH Dehydrogenase / Q000235:genetics*; D010140:Pacific Ocean; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D029941:Fish Proteins; D009245:NADH Dehydrogenase