title: Oral tolerance and OVA-induced tolerogenic dendritic cells reduce the severity of collagen/ovalbumin-induced arthritis in mice.
abstract: Dietary proteins play an important role in the regulation of systemic immune response, in a phenomenon known as oral tolerance (OT). To evaluate the effects of OT on a murine model of type II collagen (CII) plus ovalbumin (OVA)-induced arthritis (CIA), mice were fed with OVA either before or after CIA induction. OT significantly reduced the paw edema and synovial inflammation, as well as serum levels of anti-CII, the ex vivo proliferation and inflammatory cytokine production by spleen cells from CIA mice. The frequencies of Foxp3(+) and IL-10(+) cells were higher, whereas IFNγ(+) cells and IL-17(+) cells were lower, among gated CD4(+) spleen T cells from tolerized CIA mice than in those from non-tolerized CIA mice. Adoptive transfer of tolerogenic dendritic cells (DCs) before CIA induction mimics the effects observed in the OT. We demonstrate here that bystander suppression induced by OT can modify the course of CIA and tolerogenic DCs play a role this phenomenon.
authors.affiliation: Department of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas, Rua Monteiro Lobato, SP, Brazil. rodolfo.thome@gmail.com
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Thomé
authors.forename: Luis Gustavo Romani
authors.initials: LG
authors.lastname: Fernandes
authors.forename: Marcela Franco
authors.initials: MF
authors.lastname: Mineiro
authors.forename: Patricia Ucelli
authors.initials: PU
authors.lastname: Simioni
authors.forename: Paulo Pinto
authors.initials: PP
authors.lastname: Joazeiro
authors.forename: Wirla Maria da Silva Cunha
authors.initials: WM
authors.lastname: Tamashiro
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D001169:Arthritis, Experimental / Q000276:immunology / Q000473:pathology / Q000517:prevention & control / Q000628:therapy*; D024201:Bystander Effect; D018920:Coculture Techniques; D024043:Collagen Type II / Q000276:immunology / Q000633:toxicity; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000276:immunology / Q000637:transplantation; D004044:Dietary Proteins / Q000276:immunology / Q000627:therapeutic use*; D004487:Edema / Q000209:etiology; D051858:Forkhead Transcription Factors / Q000032:analysis; D007108:Immune Tolerance; D007114:Immunization; D007371:Interferon-gamma; D016753:Interleukin-10; D020381:Interleukin-17; D007518:Isoantibodies / Q000097:blood / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology / Q000627:therapeutic use* / Q000633:toxicity; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000276:immunology / Q000473:pathology
chemical_list: D024043:Collagen Type II; D016207:Cytokines; D004044:Dietary Proteins; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; C556062:IL10 protein, mouse; D020381:Interleukin-17; D007518:Isoantibodies; D016753:Interleukin-10; D007371:Interferon-gamma; D010047:Ovalbumin