title: [Prevalence of seronegative hepatitis D among patients with chronic viral hepatitis B].
abstract: OBJECTIVE
  Evaluate prevalence of hepatitis D virus replication markers among patients with chronic viral hepatitis B (CVHB) with active infection course and pronounced biochemical markers of liver damage (ALT level increase).
  
  
  METHODS
  ALT>2N served as a criteria of active CVHB course. The following parameters were determined: HBsAg, anti-HBcor IgG, anti-HBeAb, anti-HCV IgG, anti-HDV IgG+IgM, HBV DNA, HDV RNA. HCV IgG(+) patients were excluded from the analysis. In total 142 patients were examined.
  
  
  RESULTS
  Antibodies against HDV were detected in 16.2% (n=23) with CVHB, and HDV DNAwas detected in 21.8% of the examined individuals (n=31). The following variants of HDV infection marker combination were detected: HDV IgG(-) HDV RNA(-) - 75.3% (n=107), no HDV infection; HDV IgG(+) HDV RNA(-) - 2.8% (n=4), anamnestic antibodies against HDV; HDV IgG(+) HDV RNA (+) - 13.4% (n=19), active CVHB+D infection; HDV IgG(-) HDV RNA(+) - 8.4% (n=12), seronegative course of CVHB+B.
  
  
  CONCLUSIONS
  Examination of patients with pronounced cytolytic syndrome but PCR HBV DNA (-) must include not only determination of serologic markers of HDV infection but also HDV replication markers (PCR HDV RNA). Detection ofpatients with seronegative HDVcourse among patients with HVHB (8.4%) persuasively demonstrates the necessityto introduce molecular-biological examination for HDV RNA into CVHB laboratory diagnostics algorithm.
authors.forename: A V
authors.initials: AV
authors.lastname: Semenov
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D005260:Female; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006513:Hepatitis B e Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000276:immunology; D019694:Hepatitis B, Chronic / Q000097:blood* / Q000175:diagnosis / Q000453:epidemiology* / Q000276:immunology; D003699:Hepatitis D / Q000097:blood* / Q000175:diagnosis / Q000453:epidemiology* / Q000276:immunology; D003698:Hepatitis Delta Virus / Q000276:immunology; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012367:RNA, Viral / Q000097:blood*; D012426:Russia / Q000453:epidemiology
chemical_list: D000914:Antibodies, Viral; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D012367:RNA, Viral