title: Effect of ischemia preconditioning on renal ischemia/reperfusion injury in rats.
abstract: OBJECTIVE
  To study the effect of ischemia preconditioning (IP) on renal ischemia/reperfusion (I/R)-associated functional injury and expression of renal adhesion molecules in rats.
  
  
  METHODS
  The ischemia preconditioning plan adopted in this experiment involved renal warm ischemia for 6 min. and blood flow for 4 min., repeated four times. The Wistar rat kidneys used for warm ischemia preconditioning were subjected to 60 min of renal warm ischemia followed by reperfusion. The rat kidneys with ischemia/reperfusion were compared with the ischemia preconditioning group to observe rat renal function and changes in the expression of renal adhesion molecules ICAM-1, P-Selectin, and E-Selectin.
  
  
  RESULTS
  The expression of rat renal adhesion molecules (ICAM-1, P-Selectin, and E-Selectin) with ischemia preconditioning was significantly lower than that of the ischemia/reperfusion group. Serum creatinine was significantly lower than that in the ischemia/reperfusion group after 48 hours.
  
  
  CONCLUSIONS
  Ischemia preconditioning has a protective effect on renal function. Reduced expression of renal adhesion molecules is likely a mechanism involved in the observed protection.
authors.affiliation: Department of Urology, The General Hospital of Shenyang Military Region, Shenyang, China.
authors.forename: Lian-hui
authors.initials: LH
authors.lastname: Fan
authors.forename: Long
authors.initials: L
authors.lastname: He
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Cao
authors.forename: Bjun
authors.initials: B
authors.lastname: Xiang
authors.forename: Long
authors.initials: L
authors.lastname: Liu
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000032:analysis; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D019040:E-Selectin / Q000032:analysis; D005260:Female; D007150:Immunohistochemistry; D019194:Ischemic Preconditioning / Q000379:methods*; D007668:Kidney / Q000098:blood supply* / Q000473:pathology; D008297:Male; D019007:P-Selectin / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D013997:Time Factors
chemical_list: D015815:Cell Adhesion Molecules; D019040:E-Selectin; D019007:P-Selectin; D003404:Creatinine