title: A critical role for protein degradation in the nucleus accumbens core in cocaine reward memory.
abstract: The intense associative memories that develop between cocaine-paired contexts and rewarding stimuli contribute to cocaine seeking and relapse. Previous studies have shown impairment in cocaine reward memories by manipulating a labile state induced by memory retrieval, but the mechanisms that underlie the destabilization of cocaine reward memory are unknown. In this study, using a Pavlovian cocaine-induced conditioned place preference (CPP) procedure in rats, we tested the contribution of ubiquitin-proteasome system-dependent protein degradation in destabilization of cocaine reward memory. First, we found that polyubiquitinated protein expression levels and polyubiquitinated N-ethylmaleimide-sensitive fusion (NSF) markedly increased 15 min after retrieval while NSF protein levels decreased 1 h after retrieval in the synaptosomal membrane fraction in the nucleus accumbens (NAc) core. We then found that infusion of the proteasome inhibitor lactacystin into the NAc core prevented the impairment of memory reconsolidation induced by the protein synthesis inhibitor anisomycin and reversed the effects of anisomycin on NSF and glutamate receptor 2 (GluR2) protein levels in the synaptosomal membrane fraction in the NAc core. We also found that lactacystin infusion into the NAc core but not into the shell immediately after extinction training sessions inhibited CPP extinction and reversed the extinction training-induced decrease in NSF and GluR2 in the synaptosomal membrane fraction in the NAc core. Finally, infusions of lactacystin by itself into the NAc core immediately after each training session or before the CPP retrieval test had no effect on the consolidation and retrieval of cocaine reward memory. These findings suggest that ubiquitin-proteasome system-dependent protein degradation is critical for retrieval-induced memory destabilization.
authors.affiliation: Pharmacy Department, Peking University Third Hospital, Beijing, China.
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Ren
authors.forename: Meng-Meng
authors.initials: MM
authors.lastname: Liu
authors.forename: Yan-Xue
authors.initials: YX
authors.lastname: Xue
authors.forename: Zeng-Bo
authors.initials: ZB
authors.lastname: Ding
authors.forename: Li-Fen
authors.initials: LF
authors.lastname: Xue
authors.forename: Suo-Di
authors.initials: SD
authors.lastname: Zhai
authors.forename: Lin
authors.initials: L
authors.lastname: Lu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D003042:Cocaine / Q000008:administration & dosage*; D003216:Conditioning, Operant / Q000187:drug effects / Q000502:physiology; D018765:Dopamine Uptake Inhibitors / Q000008:administration & dosage*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005108:Extinction, Psychological / Q000187:drug effects / Q000502:physiology; D008297:Male; D008568:Memory / Q000187:drug effects / Q000502:physiology*; D009714:Nucleus Accumbens / Q000187:drug effects / Q000378:metabolism*; D025821:Polyubiquitin / Q000378:metabolism; D059748:Proteolysis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000378:metabolism; D012201:Reward; D013997:Time Factors; D025801:Ubiquitin / Q000378:metabolism
chemical_list: D018765:Dopamine Uptake Inhibitors; D004791:Enzyme Inhibitors; D018091:Receptors, AMPA; D025801:Ubiquitin; D025821:Polyubiquitin; D003042:Cocaine; C104722:glutamate receptor ionotropic, AMPA 2