title: Detection of carcinoma micrometastases in bone marrow of dogs and cats using conventional and cell block cytology.
abstract: BACKGROUND
  The presence of human breast carcinoma micrometastases in bone marrow is associated with poor overall survival, poor breast-cancer-specific survival, poor disease-free survival, and poor distant disease-free survival. In veterinary practice, the detection of micrometastases as a component of clinical staging is a routine practice for lymphomas and mast cell tumors, but not for carcinomas.
  
  
  OBJECTIVE
  This prospective study evaluated whether the identification of micrometastases from various carcinomas in dogs and cats in bone marrow using cell block cytology is technically feasible and whether it correlates with routine cytologic examination.
  
  
  METHODS
  Thirteen dogs and 4 cats with various types of carcinomas were available for analysis. Routine and cell block cytologic evaluation combined with immunocytochemical staining with antibodies to CKAE1/AE3 and CK7 were performed on all bone marrow samples.
  
  
  RESULTS
  Bone marrow micrometastasis was demonstrated by both methods in 2 dogs with advanced disease. In one case cells were immunoreactive for both CKAE1/AE3 and CK7.
  
  
  CONCLUSIONS
  This study demonstrates that cell block cytology is a practical and useful method for bone marrow evaluation and is suitable for cytokeratin immunocytochemical analysis.
authors.affiliation: Donaldson-Atwood Cancer Center, The Animal Medical Center, New York, NY, USA. betf84@hotmail.com
authors.forename: Bonnie E
authors.initials: BE
authors.lastname: Taylor
authors.forename: Nicole F
authors.initials: NF
authors.lastname: Leibman
authors.forename: Richard
authors.initials: R
authors.lastname: Luong
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Loar
authors.forename: Diane M
authors.initials: DM
authors.lastname: Craft
mesh_terms: D000694:Anal Gland Neoplasms / Q000473:pathology; D000695:Anal Sacs; D000818:Animals; D019046:Bone Marrow Neoplasms / Q000556:secondary / Q000662:veterinary*; D002277:Carcinoma / Q000473:pathology / Q000662:veterinary*; D002371:Cat Diseases / Q000175:diagnosis / Q000473:pathology*; D002415:Cats; D003584:Cytological Techniques / Q000379:methods / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis / Q000473:pathology*; D004285:Dogs; D005260:Female; D008175:Lung Neoplasms / Q000473:pathology / Q000662:veterinary; D008297:Male; D061206:Neoplasm Micrometastasis; D011446:Prospective Studies; D012878:Skin Neoplasms / Q000473:pathology / Q000662:veterinary; D013544:Sweat Gland Neoplasms / Q000473:pathology / Q000662:veterinary; D013964:Thyroid Neoplasms / Q000473:pathology / Q000662:veterinary; D014067:Tonsillar Neoplasms / Q000473:pathology / Q000662:veterinary; D014625:Vaginal Neoplasms / Q000473:pathology / Q000662:veterinary