title: Role of serotonin receptors in regulation of contractile activity of urinary bladder in rabbits.
abstract: OBJECTIVE
  To evaluate the role of different serotonin (5-hydroxytryptamine [5-HT]) receptor subtypes on urinary bladder contraction, pharmacologic analysis of electromotor activity (EMA) variation was performed using a rabbit bladder model.
  
  
  METHODS
  Measurements of EMA were performed on 3 urinary bladder portions: top, body, and trigone. The experiments were performed on 24 Shinshilla rabbits of both sexes, 5-6 months old, and weighing 2.5-4.0 kg. The bladder was isolated. Noninvasive electrodes were superimposed on the surface of the top, body, and trigone of the bladder. Contact between the electrode tips and the bladder surface was achieved. The bladder EMA was measured using bipolar silver electrodes for extracellular recordings.
  
  
  RESULTS
  The stimulation of the serotoninergic fibers and parasympathetic nerve resulted in increased bladder EMA frequency and amplitude (72% and 25%, respectively). The increase in bladder EMA was prevented by administration of selective inhibitors of serotonin receptor subtypes such as droperidol, spiperone, and sumatriptan. Exogenous serotonin administered to the rabbits after vagus nerve excitation increased the typical EMA of the bladder body smooth muscle.
  
  
  CONCLUSIONS
  The serotoninergic system has been widely implicated in the control of urinary bladder function. In the present study, we have demonstrated that preganglionic fibers and ganglionic serotoninergic neurons, expressing the 5-HT3 and 5-HT4 receptors, and the effector smooth muscle cells, expressing 5-HT1 and 5-HT2 receptors, are actively involved in the regulation of the bladder contractile activity in rabbits.
authors.affiliation: Central Research Institute of Gastroenterology, Moscow, Russia.
authors.forename: Alla E
authors.initials: AE
authors.lastname: Lychkova
authors.forename: Luigi Michele
authors.initials: LM
authors.lastname: Pavone
mesh_terms: D000818:Animals; D005260:Female; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D011817:Rabbits; D011985:Receptors, Serotonin / Q000187:drug effects / Q000502:physiology*; D012702:Serotonin Antagonists / Q000494:pharmacology; D001743:Urinary Bladder / Q000187:drug effects / Q000502:physiology*
chemical_list: D011985:Receptors, Serotonin; D012702:Serotonin Antagonists