title: The tetraspanin CD151-ARSA mutant inhibits angiogenesis via the YRSL             sequence.
abstract: Previous studies have shown that the tetraspanin CD151 is essential for             pathological or physiological angiogenesis. However, the cellular signaling mechanism             and the role of the CD151 YRSL sorting motif in in vitro vasculogenesis remains             unknown. In this study, the results showed that both CD151 and CD151-ARSA gene             delivery were capable of increasing the expression of CD151 at the protein level             in human umbilical vein endothelial cells (HUVECs). Moreover, there was no significant             difference in CD151 protein expression between the CD151 group and the CD151-ARSA             group. Overexpression of CD151 promoted HUVEC cell proliferation, migration and             capillary network formation in vitro. However, in the CD151-ARSA group, the abilities             of cell proliferation, migration and capillary network formation were all decreased,             compared with the CD151 group. Furthermore, the activation of PI3K, Akt and ERK             signaling pathways was attenuated in the CD151-ARSA mutant group compared with             the CD151 group. This study suggests that the YRSL motif of CD151 plays a key             role in CD151-induced angiogenesis. Our observations provide insights into a new             mechanism of CD151 regulating angiogenesis via vesicle trafficking.
authors.affiliation: Department of Nuclear Medicine of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Peng
authors.forename: Houjuan
authors.initials: H
authors.lastname: Zuo
authors.forename: Zhengxiang
authors.initials: Z
authors.lastname: Liu
authors.forename: Jin
authors.initials: J
authors.lastname: Qin
authors.forename: Yuanlin
authors.initials: Y
authors.lastname: Zhou
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Li
authors.forename: Daowen
authors.initials: D
authors.lastname: Wang
authors.forename: Hesong
authors.initials: H
authors.lastname: Zeng
authors.forename: Xin A
authors.initials: XA
authors.lastname: Zhang
mesh_terms: D020816:Amino Acid Motifs; D002465:Cell Movement; D049109:Cell Proliferation; D000229:Dependovirus / Q000235:genetics; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D009154:Mutation; D018919:Neovascularization, Physiologic / Q000502:physiology*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction; D060185:Tetraspanin 24 / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D060185:Tetraspanin 24; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases