title: Knockdown of Coronin-1C disrupts Rac1 activation and impairs tumorigenic             potential in hepatocellular carcinoma cells.
abstract: Coronin-1C is an important F-actin binding protein which is critical for             cell motility. Furthermore, the expression of this protein was found to be increased             in diffuse tumors and was correlated with the degree of tumor malignancy. However,             the mechanism(s) through which this protein enhances malignancy in hepatocellular             carcinoma (HCC) is poorly understood. In this study, we found that Coronin-1C             was overexpressed in human HCC tissues compared with the adjacent non-tumor tissues.             Overexpression of Coronin-1C enhanced the cell migration in the human HCC cell             line BEL-7402, whereas suppressed cell migration and proliferation were observed             in Coronin-1C-knockdown BEL-7402 cells together with impaired cell polarity, disrupted             cytoskeleton and decreased Rac-1 activation. Moreover, the Coronin-1C knockdown             cells displayed a lower degree of malignancy by inducing smaller tumors in nude             mice. Thus, we demonstrated a relationship between Coronin-1C overexpression and             human HCC growth through enhancement of tumor cell proliferation and migration,             which are correlated with Rac-1 activation.
authors.affiliation: The Second Affiliated Hospital of Jilin University, Changchun, Jilin 130041, PR China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Wang
authors.forename: Ming-Ku
authors.initials: MK
authors.lastname: Jia
authors.forename: Hong
authors.initials: H
authors.lastname: Cao
authors.forename: Peng
authors.initials: P
authors.lastname: Bian
authors.forename: Xue-Dong
authors.initials: XD
authors.lastname: Fang
mesh_terms: D000368:Aged; D000818:Animals; D063646:Carcinogenesis / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D016764:Cell Polarity; D049109:Cell Proliferation; D003599:Cytoskeleton / Q000378:metabolism; D004789:Enzyme Activation; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D047368:Tumor Burden; D020830:rac1 GTP-Binding Protein / Q000378:metabolism*
chemical_list: D008840:Microfilament Proteins; C107953:RAC1 protein, human; C072061:coronin proteins; D020830:rac1 GTP-Binding Protein