title: Fluorescent live-cell imaging of metabolically incorporated unnatural cyclopropene-mannosamine derivatives.
abstract: Sugar coated: We recently developed methylcyclopropenes as low-molecular-weight tetrazine coupling partners. Here, we demonstrate that methylcyclopropenes can meet the stringent steric demands required for metabolic imaging of unnatural mannosamines on live cells. Using sequential azide-alkyne chemistry, we also demonstrate multicolor imaging of two different metabolically incorporated unnatural sugars.
authors.affiliation: Department of Chemistry and Biochemistry University of California, San Diego 9500 Gilman Dr, La Jolla, CA 92093 (USA).
authors.forename: Christian M
authors.initials: CM
authors.lastname: Cole
authors.affiliation: Department of Chemistry and Biochemistry University of California, San Diego 9500 Gilman Dr, La Jolla, CA 92093 (USA).
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Chemistry and Biochemistry University of California, San Diego 9500 Gilman Dr, La Jolla, CA 92093 (USA).
authors.forename: Jolita
authors.initials: J
authors.lastname: Šečkutė
authors.affiliation: Department of Chemistry and Biochemistry University of California, San Diego 9500 Gilman Dr, La Jolla, CA 92093 (USA).
authors.forename: Neal K
authors.initials: NK
authors.lastname: Devaraj
mesh_terms: D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival; D057930:Click Chemistry; D061565:Cycloaddition Reaction; D003521:Cyclopropanes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006595:Hexosamines / Q000032:analysis*; D006801:Humans; D018613:Microscopy, Confocal
chemical_list: D000480:Alkynes; D001386:Azides; D003521:Cyclopropanes; D005456:Fluorescent Dyes; D006595:Hexosamines; C008322:mannosamine; C021985:cyclopropene