title: Protective role of quercetin on polychlorinated biphenyls (Aroclor-1254) induced oxidative stress and apoptosis in liver of adult male rats.
abstract: The present study aims to investigate the protective effect of quercetin against Aroclor-1254-induced hepatotoxicity in rats. Male Wistar rats were grouped into Group I control received vehicle (corn oil; 1 mL/kg bwt); Group II quercetin alone (50 mg/kg bwt/day orally); Group III Aroclor-1254 (2 mg/kg bwt/day intraperitoneally); Group IV Aroclor-1254 + quercetin treated for 30 days. The Aroclor-1254 treatment caused significant alteration in the biochemical parameters (hydrogen peroxide, lipid peroxidation, reduced glutathione levels, and alkaline phosphatase activity). The expressions of apoptotic and antiapoptotic proteins and the liver histology of Aroclor-1254-exposed rats showed cytoplasmic degeneration along with infiltration of polymorphonuclear cells. Whereas simultaneous treatment with quercetin normalized all the biochemical parameters, consequently it inhibited apoptosis mediated by Aroclor-1254 by downregulating aryl hydrocarbon receptor, p53 and apoptotic protein (Bax, caspase-9, caspase-3) and upregulating the antiapoptotic protein (Bcl-2) expression patterns; thereby, quercetin reduces alteration in hepatocellular morphology. Thus quercetin exhibited hepatoprotective effect.
authors.affiliation: Department of Endocrinology, Dr. A. L. M. PG Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, 600 113, India.
authors.forename: Suganya
authors.initials: S
authors.lastname: Sekaran
authors.forename: Selvakumar
authors.initials: S
authors.lastname: Kandaswamy
authors.forename: Krishnamoorthy
authors.initials: K
authors.lastname: Gunasekaran
authors.forename: Elumalai
authors.initials: E
authors.lastname: Perumal
authors.forename: Fariya Yasmine
authors.initials: FY
authors.lastname: Afsar Basha
authors.forename: Balasakthi Janani
authors.initials: BJ
authors.lastname: Madhan Mohan
authors.forename: Arunakaran
authors.initials: A
authors.lastname: Jagadeesan
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D020111:Chlorodiphenyl (54% Chlorine) / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D011794:Quercetin / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D018336:Receptors, Aryl Hydrocarbon / Q000235:genetics
chemical_list: D018336:Receptors, Aryl Hydrocarbon; D020111:Chlorodiphenyl (54% Chlorine); D011794:Quercetin; D000469:Alkaline Phosphatase