title: [Detection and clinical significance of vascular endothelium growth factor and microvessel density in patients with nasopharyngeal carcinoma].
abstract: OBJECTIVE
  To investigate the role of VEGF in angiogenesis of nasopharyngeal carcinoma tissue.
  
  
  METHODS
  Serum and tissue VEGF were detected by the quantitative enzyme-linked immunosorbent assay (ELISA) method both in 62 nasopharyngeal carcinoma without therapy and 20 nasopharyngitis. The expression of microvessel density in tissue of nasopharyngeal carcinoma and nasopharyngitis were detected by immunohistochemical method.
  
  
  RESULTS
  (1) There was no significant relationship compared VEGF and MVD in nasopharyngeal carcinoma patient, gender and ages (P>0.05), while the expression of VEGF and MVD in later stage (III+IV) were significant higher than that in earlier stage (I+I) (P<0.01), and in positive lymph node group or metastasis group were higher than in negative group (P<0.01); (2) The expression of VEGF and MVD in nasopharyngeal carcinoma serum and tissue were higher than in nasopharyngitis (P<0.05); (3) There was positive correlation between VEGF and MVD in nasopharyngeal carcinoma tissue (r=0.865, P<0.01), but negative correlation in their serum (r=0.328, P>0.05).
  
  
  CONCLUSIONS
  VEGF could induce angiogenesis in nasopharyngeal carcinoma tissue, and play an important role in progression in nasopharyngeal carcinoma, VEGF could be an important marker for monitoring prognosis of disease.
authors.affiliation: Head and Neck Tumor Research Center of Third Affiliated Hospital of Kunming Medical University, Kunming, 650118, China.
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Ren
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Yanling
authors.initials: Y
authors.lastname: Tu
authors.forename: Jinpeng
authors.initials: J
authors.lastname: Wu
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
authors.forename: Ruimei
authors.initials: R
authors.lastname: Sun
authors.forename: Jun
authors.initials: J
authors.lastname: Sui
authors.forename: Xiaojiang
authors.initials: X
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002277:Carcinoma; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D055806:Microvessels; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000098:blood supply* / Q000378:metabolism* / Q000473:pathology; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic; D011379:Prognosis; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D055815:Young Adult
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A