title: Characterization of a fluorescence probe based on gold nanoclusters for cell and animal imaging.
abstract: A facile approach to synthesize gold nanoclusters (Au NCs) with bluish green fluorescence using histidine as both reductant and capping agent was reported. The UV-visible absorption and photoluminescence spectra measurement was performed to explore its optical properties under different circumstances (preparing condition, temperature, pH, storing time). Then, MPA, a NIR organic dye, was conjugated to Au NCs (Au-MPA) for in vivo fluorescence imaging application. Low cytotoxicity and high affinity to tumor of this nanoprobe was proved at the cellular level, and its bio-distribution in normal nude mice and MCF-7 tumor-bearing mice was also investigated. Consequently, the results demonstrated the promising potential of the green Au NCs conjugated with NIR dye as nanoprobes in bioimaging and related fields.
authors.affiliation: Department of Biomedical Engineering, State Key Laboratory of Natural Medicines and School of Life Science and Technology, China Pharmaceutical University, 24 Tongjia Lane, Gulou District, Nanjing 210009, People's Republic of China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Chen
authors.forename: Bowen
authors.initials: B
authors.lastname: Li
authors.forename: Chuan
authors.initials: C
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhengqi
authors.initials: Z
authors.lastname: Cheng
authors.forename: Xi
authors.initials: X
authors.lastname: Dai
authors.forename: Rui
authors.initials: R
authors.lastname: Zhu
authors.forename: Yueqing
authors.initials: Y
authors.lastname: Gu
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D006046:Gold / Q000737:chemistry* / Q000633:toxicity; D056945:Hep G2 Cells; D006801:Humans; D057446:Hydrodynamics; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D061848:Optical Imaging / Q000379:methods*; D010316:Particle Size; D013056:Spectrophotometry, Ultraviolet; D019265:Spectroscopy, Near-Infrared; D013696:Temperature; D023041:Xenograft Model Antitumor Assays
chemical_list: D005456:Fluorescent Dyes; D006046:Gold