title: The combined effects of genetic variation in the SIRT1 gene and dietary intake of n-3 and n-6 polyunsaturated fatty acids on serum LDL-C and HDL-C levels: a population based study.
abstract: BACKGROUND
  Dyslipidemia due to high total cholesterol, LDL-cholesterol, triglycerides, or low HDL-cholesterol is an important risk factor for coronary heart disease (CHD). Both SIRT1 and PUFAs can influence the expression of genes for nuclear receptors and transcription factors related to lipid metabolism such as LXRα, LXRβ, PPARα, SREBP-1c.
  
  
  METHODS
  A total of 707 Japanese males and 723 females were randomly selected from the participants who visited a medical center for routine medical check-ups. We analyzed the combined effects of the genotype/haplotype of the SIRT1 gene and dietary n-6/n-3 PUFA intake ratio on the determination of serum lipid levels.
  
  
  RESULTS
  We found that the SIRT1 gene marked with haplotype 2 was associated with decreased serum LDL-cholesterol and increased HDL-cholesterol levels. In addition, the associations between the SIRT1 haplotype 2 and decreased LDL-C and increased HDL-C levels were only observed in the low n-6/n-3 PUFA intake ratio group, but not in the high n-6/n-3 PUFA intake ratio group.
  
  
  CONCLUSIONS
  Our findings indicate that the combination of genetic variation in the SIRT1 gene and dietary n-6 and/or n-3 PUFA intake influence the determination of inter-individual variations of serum levels of LDL-C and HDL-C.
authors.affiliation: Laboratory of Human Genetics, School of Food and Nutritional Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka, Shizuoka, 422-8526, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Inamori
authors.forename: Toshinao
authors.initials: T
authors.lastname: Goda
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Kasezawa
authors.forename: Kimiko
authors.initials: K
authors.lastname: Yamakawa-Kobayashi
mesh_terms: D000368:Aged; D008076:Cholesterol, HDL / Q000097:blood*; D008078:Cholesterol, LDL / Q000097:blood*; D004041:Dietary Fats / Q000008:administration & dosage / Q000097:blood*; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage / Q000097:blood*; D043371:Fatty Acids, Omega-6 / Q000008:administration & dosage / Q000097:blood*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D014644:Genetic Variation; D006239:Haplotypes; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism*; D014280:Triglycerides / Q000097:blood
chemical_list: D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D004041:Dietary Fats; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D014280:Triglycerides; C447939:SIRT1 protein, human; D056564:Sirtuin 1