title: Antioxidant activity and oxidative stress protection of duck proteins hydrolysates in SK-N-SH cells.
abstract: Studies have found that natural antioxidants, which are free-radical scavengers, can reduce the risk of diseases caused by free radicals. This work investigated the antioxidant properties of duck proteins hydrolysates. The free-radical scavenging function of CP-1 (M(r) > 10 kDa), CP-2 (5 kDa < M(r) < 10 kDa) and CP-3 (M(r) < 5 kDa), obtained through ultrafiltration and gel filtration were evaluated. The results showed that the lower molecular weight fraction exhibited a stronger free-radical scavenging ability. The highest free-radical scavenging activity was detected in the fraction of p4 purified from CP-3 using Sephadex G-15 column chromatography. The 50% inhibitory value (IC(50)) of p4 for scavenging radicals of superoxide, hydroxyl and 1,1-diphenyl-2-pycrylhydrazyl (DPPH) were, respectively, 0.97 mg mL(-1), 0.84 mg mL(-1) and 1.84 mg mL(-1). Furthermore, the p4 fraction at a concentration of 10 μg mL(-1) increased cell viability from 84.8% to 94% under antioxidative stress in neuroblastoma SK-N-SH cells.
authors.affiliation: Food Science and Nutrition Department, Ginling College, Nanjing Normal University, Nanjing 210097, P R China.
authors.forename: Yuxing
authors.initials: Y
authors.lastname: Guo
authors.forename: Daodong
authors.initials: D
authors.lastname: Pan
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wu
authors.forename: Chuanchuan
authors.initials: C
authors.lastname: Zhao
authors.forename: Jinxuan
authors.initials: J
authors.lastname: Cao
mesh_terms: D000818:Animals; D001713:Biphenyl Compounds / Q000494:pharmacology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004372:Ducks; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005609:Free Radicals / Q000009:adverse effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000009:adverse effects; D017665:Hydroxyl Radical / Q000494:pharmacology; D008970:Molecular Weight; D018384:Oxidative Stress / Q000187:drug effects*; D010851:Picrates / Q000494:pharmacology; D011492:Protein Hydrolysates / Q000494:pharmacology*; D013481:Superoxides / Q000494:pharmacology
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D010851:Picrates; D011492:Protein Hydrolysates; D013481:Superoxides; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl