title: Fluoroquinolone-resistant extraintestinal pathogenic Escherichia coli, including O25b-ST131, isolated from faeces of hospitalized dogs in an Australian veterinary referral centre.
abstract: OBJECTIVE
  To determine rates of carriage of fluoroquinolone-resistant Escherichia coli and extraintestinal pathogenic E. coli (ExPEC) among dogs in a specialist referral hospital and to examine the population structure of the isolates.
  
  
  METHODS
  Fluoroquinolone-resistant faecal E. coli isolates (n = 232, from 23 of 123 dogs) recovered from hospitalized dogs in a veterinary referral centre in Sydney, Australia, over 140 days in 2009 were characterized by phylogenetic grouping, virulence genotyping and random amplified polymorphic DNA (RAPD) analysis.
  
  
  RESULTS
  The RAPD dendrogram for representative isolates showed one group B2-associated cluster and three group D-associated clusters; each contained isolates with closely related ExPEC-associated virulence profiles. All group B2 faecal isolates represented the O25b-ST131 clonal group and were closely related to recent canine extraintestinal ST131 clinical isolates from the east coast of Australia by RAPD analysis.
  
  
  CONCLUSIONS
  Hospitalized dogs may carry fluoroquinolone-resistant ExPEC in their faeces, including those representing O25b-ST131.
authors.affiliation: School of Veterinary Science, University of Queensland, Gatton Campus, QLD 4343, Australia.
authors.forename: Siyu
authors.initials: S
authors.lastname: Guo
authors.forename: Huub J M
authors.initials: HJ
authors.lastname: Brouwers
authors.forename: Rowland N
authors.initials: RN
authors.lastname: Cobbold
authors.forename: Joanne L
authors.initials: JL
authors.lastname: Platell
authors.forename: Toni A
authors.initials: TA
authors.lastname: Chapman
authors.forename: Vanessa R
authors.initials: VR
authors.lastname: Barrs
authors.forename: James R
authors.initials: JR
authors.lastname: Johnson
authors.forename: Darren J
authors.initials: DJ
authors.lastname: Trott
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001315:Australia / Q000453:epidemiology; D002353:Carrier State / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D016000:Cluster Analysis; D004285:Dogs; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D024841:Fluoroquinolones / Q000494:pharmacology*; D006762:Hospitals, Animal; D017720:Molecular Epidemiology; D058889:Molecular Typing; D010802:Phylogeny; D015995:Prevalence; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones