title: The properties of human CD40-activated B cells as antigen-presenting             cells are not affected by PGE2.
abstract: Tumor vaccination represents a promising immuno-therapeutic strategy in             cancer. However, the inherent ability of many tumors to evade immune responses             by suppression of immune cell function represents a major barrier. Prostaglandin E2             (PGE2) has been shown to be a critical tumor-derived immunosuppressive factor.             It affects a broad range of immune cells including T cells, macrophages and dendritic             cells (DCs). CD40-activated B cells are being studied as a potential alternative             to DCs as antigen-presenting cells for immunotherapy. So far, it is not known             whether PGE2 affects their antigen presenting capacity. We, therefore, investigated             the influence of PGE2 on the phenotype, migratory potential and antigen-presenting             function of CD40-activated human B cells. Here, we demonstrate that the immunostimulatory             properties of CD40-activated B cells are not affected by PGE2. These results support             the use of CD40-activated B cells as cellular adjuvants, especially in settings             where PGE2 is present in the tumor microenvironment.
authors.affiliation: Cologne Interventional Immunology (CII), Department I of Internal                     Medicine, University Hospital of Cologne, D-50924 Cologne, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Shimabukuro-Vornhagen
authors.forename: Andreas
authors.initials: A
authors.lastname: Draube
authors.forename: Tanja
authors.initials: T
authors.lastname: Liebig
authors.forename: Alexey
authors.initials: A
authors.lastname: Popov
authors.forename: Achim
authors.initials: A
authors.lastname: Rothe
authors.forename: Michael
authors.initials: M
authors.lastname: von Bergwelt-Baildon
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D019013:CD40 Antigens / Q000378:metabolism*; D049109:Cell Proliferation; D048430:Cell Shape; D002633:Chemotaxis; D018920:Coculture Techniques; D015232:Dinoprostone / Q000502:physiology*; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D010641:Phenotype; D054400:Receptors, CCR7 / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000378:metabolism; D058307:Receptors, Prostaglandin E, EP2 Subtype / Q000378:metabolism; D058309:Receptors, Prostaglandin E, EP4 Subtype / Q000378:metabolism
chemical_list: C515370:CCR7 protein, human; D019013:CD40 Antigens; C527276:CXCR4 protein, human; C545644:PTGER2 protein, human; D054400:Receptors, CCR7; D019718:Receptors, CXCR4; D058307:Receptors, Prostaglandin E, EP2 Subtype; D058309:Receptors, Prostaglandin E, EP4 Subtype; D015232:Dinoprostone