title: Dual-wavelength fluorescent detection of particles on a novel microfluidic chip.
abstract: This paper reports a novel lab-on-a-chip device that performs fluorescent particle counting by coupling the electrokinetically-induced pressure-driven flow and a miniaturized dual-wavelength fluorescent detection method. A novel L-shaped PDMS microchannel bonded on a thin glass slide is used to transport the particles. The dual-wavelength fluorescent detection system can count two different fluorescent particles simultaneously. Good agreement is achieved between the results obtained by the microfluidic chip device and the results from a commercial flow cytometer.
authors.affiliation: Department of Mechanical and Mechatronics Engineering, University of Waterloo, Waterloo, Ontario, Canada.
authors.forename: Hai
authors.initials: H
authors.lastname: Jiang
authors.forename: Xuan
authors.initials: X
authors.lastname: Weng
authors.forename: Dongqing
authors.initials: D
authors.lastname: Li
mesh_terms: D004129:Dimethylpolysiloxanes / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D011312:Pressure
chemical_list: D004129:Dimethylpolysiloxanes; D005456:Fluorescent Dyes; C013830:baysilon