title: An amphiphilic perylene imido diester for selective cellular imaging.
abstract: A new amphiphilic membrane marker based on a water-soluble dendritic polyglycerol perylene imido dialkylester has been designed, synthesized, and its optical properties characterized. In water it forms fluorescently quenched micellar self-aggregates, but when incorporated into a lipophilic environment, it monomerizes, and the highly fluorescent properties of the perylene core are recovered. These properties make it an ideal candidate for the imaging of artificial and cellular membranes as demonstrated by biophysical studies.
authors.affiliation: Institut für Chemie und Biochemie, Freie Universität Berlin, Takustraße 3, 14195 Berlin, Germany.
authors.forename: Timm
authors.initials: T
authors.lastname: Heek
authors.forename: Jörg
authors.initials: J
authors.lastname: Nikolaus
authors.forename: Roland
authors.initials: R
authors.lastname: Schwarzer
authors.forename: Carlo
authors.initials: C
authors.lastname: Fasting
authors.forename: Pia
authors.initials: P
authors.lastname: Welker
authors.forename: Kai
authors.initials: K
authors.lastname: Licha
authors.forename: Andreas
authors.initials: A
authors.lastname: Herrmann
authors.forename: Rainer
authors.initials: R
authors.lastname: Haag
mesh_terms: D000818:Animals; D016466:CHO Cells; D002462:Cell Membrane / Q000648:ultrastructure*; D006224:Cricetinae; D050091:Dendrimers / Q000032:analysis* / Q000138:chemical synthesis; D005456:Fluorescent Dyes / Q000032:analysis* / Q000138:chemical synthesis; D007094:Imides / Q000032:analysis / Q000138:chemical synthesis; D008567:Membranes, Artificial; D008823:Micelles; D018613:Microscopy, Confocal; D010569:Perylene / Q000032:analysis* / Q000138:chemical synthesis; D013501:Surface-Active Agents / Q000032:analysis* / Q000138:chemical synthesis
chemical_list: D050091:Dendrimers; D005456:Fluorescent Dyes; D007094:Imides; D008567:Membranes, Artificial; D008823:Micelles; D013501:Surface-Active Agents; D010569:Perylene