title: Bioactivity guided fractionation of leaves extract of Nyctanthes arbor tristis (Harshringar) against P falciparum.
abstract: BACKGROUND
  Nyctanthes arbor-tristis (Harshringar, Night Jasmine) has been traditionally used in Ayurveda, Unani and other systems of medicine in India. The juice of its leaves has been used by various tribal populations of India in treatment of fevers resembling malaria.
  
  
  OBJECTIVE
  This work reports the antiplasmodial activity guided fractionation of Harshringar leaves extract.
  
  
  METHODS
  Crude ethanolic Harshringar leaves extract and its RPHPLC purified fractions were studied for antiplasmodial potency against 3D7 (CQ sensitive) and Dd2 (CQ resistant) strains of P.falciparum and subsequently subjected to bioassay guided fractionation using reverse phase chromatography to pursue the isolation of active fractions.
  
  
  RESULTS
  Harshringar crude leaves extract and some of its RPHPLC purified fractions exhibited promising antiplasmodial potency against 3D7 and Dd2 strains of P.falciparum.
  
  
  CONCLUSIONS
  The present study has provided scientific validity to the traditional use of leaves extract of Harshringar against malaria leading to the conclusion that this plant holds promise with respect to antimalarial phytotherapy. This is the first scientific report of antiplasmodial activity of RPHPLC fractions of Harshringar leaves extract against P.falciparum strains.
authors.affiliation: Malaria Research Laboratory, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India.
authors.forename: Pinky
authors.initials: P
authors.lastname: Kumari
authors.forename: Dinkar
authors.initials: D
authors.lastname: Sahal
authors.forename: S K
authors.initials: SK
authors.lastname: Jain
authors.forename: Virander S
authors.initials: VS
authors.lastname: Chauhan
mesh_terms: D000962:Antimalarials / Q000494:pharmacology*; D002478:Cells, Cultured; D005591:Chemical Fractionation; D004912:Erythrocytes / Q000187:drug effects* / Q000469:parasitology; D006801:Humans; D007194:India; D016778:Malaria, Falciparum / Q000188:drug therapy* / Q000469:parasitology; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D010963:Plasmodium falciparum / Q000187:drug effects*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000962:Antimalarials; D010936:Plant Extracts