title: Expression, purification and crystallization of the ectodomain of the envelope glycoprotein E2 from Bovine viral diarrhoea virus.
abstract: Bovine viral diarrhoea virus (BVDV) is an economically important animal pathogen which is closely related to Hepatitis C virus. Of the structural proteins, the envelope glycoprotein E2 of BVDV is the major antigen which induces neutralizing antibodies; thus, BVDV E2 is considered as an ideal target for use in subunit vaccines. Here, the expression, purification of wild-type and mutant forms of the ectodomain of BVDV E2 and subsequent crystallization and data collection of two crystal forms grown at low and neutral pH are reported. Native and multiple-wavelength anomalous dispersion (MAD) data sets have been collected and structure determination is in progress.
authors.affiliation: Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, England.
authors.forename: Oleg
authors.initials: O
authors.lastname: Iourin
authors.forename: Karl
authors.initials: K
authors.lastname: Harlos
authors.forename: Kamel
authors.initials: K
authors.lastname: El Omari
authors.forename: Weixian
authors.initials: W
authors.lastname: Lu
authors.forename: Jan
authors.initials: J
authors.lastname: Kadlec
authors.forename: Munir
authors.initials: M
authors.lastname: Iqbal
authors.forename: Christoph
authors.initials: C
authors.lastname: Meier
authors.forename: Andrew
authors.initials: A
authors.lastname: Palmer
authors.forename: Ian
authors.initials: I
authors.lastname: Jones
authors.forename: Carole
authors.initials: C
authors.lastname: Thomas
authors.forename: Joe
authors.initials: J
authors.lastname: Brownlie
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Grimes
authors.forename: David I
authors.initials: DI
authors.lastname: Stuart
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray / Q000379:methods; D030241:Diarrhea Virus 1, Bovine Viral / Q000737:chemistry*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D014759:Viral Envelope Proteins; C076551:gp53, bovine viral diarrhea virus