title: Oxidative folding in chloroplasts.
abstract: CONCLUSIONS
  Disulfide-bonded proteins in chloroplasts from green plants exist in the envelope and the thylakoid membrane, and in the stroma and the lumen. The formation of disulfide bonds in proteins is referred to as oxidative folding and is linked to the import and folding of chloroplast proteins as well as the assembly and repair of thylakoid complexes. It is also important in the redox regulation of enzymes and signal transfer.
  
  
  BACKGROUND
  Green-plant chloroplasts contain enzymes that can form and isomerize disulfide bonds in proteins. In Arabidopsis thaliana, four proteins are identified that are relevant for the catalysis of disulfide bond formation in chloroplast proteins. The proteins' low quantum yield of Photosystem II 1 (LQY1, At1g75690) and snowy cotyledon 2 (SCO2, At3g19220) exhibits protein disulfide isomerase activity and is suggested to function in the assembly and repair of Photosystem II (PSII), and the biogenesis of thylakoids in cotyledons, respectively. The thylakoid-located Lumen thiol oxidoreductase 1 (LTO1, At4g35760) can catalyze the formation of the disulfide bond of the extrinsic PsbO protein of PSII. In addition, the stroma-located protein disulfide isomerase PDIL1-3 (At3g54960) may have a role in oxidative folding.
  
  
  RESULTS
  Research on oxidative folding in chloroplasts plants is in an early stage and little is known about the mechanisms of disulfide bond formation in chloroplast proteins.
  
  
  CONCLUSIONS
  The close link between the import and folding of chloroplast proteins suggests that Hsp93, a component of the inner envelope's import apparatus, may have co-chaperones that can catalyze disulfide bond formation in newly imported proteins.
authors.affiliation: Department of Chemistry, Umeå University, Umeå 90187, Sweden. thomas.kieselbach@chem.umu.se
authors.forename: Thomas
authors.initials: T
authors.lastname: Kieselbach
mesh_terms: D017360:Arabidopsis / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D060365:Chloroplast Proteins / Q000737:chemistry* / Q000378:metabolism*; D002736:Chloroplasts / Q000737:chemistry* / Q000378:metabolism*; D004220:Disulfides / Q000378:metabolism; D010084:Oxidation-Reduction; D017510:Protein Folding
chemical_list: D060365:Chloroplast Proteins; D004220:Disulfides