title: AtCYP710A1 gene-mediated stigmasterol production plays a role in imparting temperature stress tolerance in Arabidopsis thaliana.
abstract: Stigmasterol and sitosterol, important sterols present in plants, are known to influence permeability and fluidity characteristics of the plasma membrane and other organellar membranes. We had previously demonstrated that the Arabidopsis Atcyp710A1 gene, which catalyzes conversion of sitosterol into stigmasterol, plays a role in plasma membrane permeability, thus influencing leakage of cellular nutrients and ions into apoplast. In this study, we investigated the role of this gene in imparting various abiotic stress tolerances in Arabidopsis. By analyzing Atcyp710a1 mutant and AtCYP710A1 overexpressor lines, we found that the AtCYP710A1 gene plays a role in imparting low and high temperature tolerance.
authors.affiliation: Plant Biology Division; The Samuel Roberts Noble Foundation; Ardmore, OK USA.
authors.forename: Muthappa
authors.initials: M
authors.lastname: Senthil-Kumar
authors.affiliation: Plant Biology Division; The Samuel Roberts Noble Foundation; Ardmore, OK USA.
authors.forename: Keri
authors.initials: K
authors.lastname: Wang
authors.affiliation: Plant Biology Division; The Samuel Roberts Noble Foundation; Ardmore, OK USA.
authors.forename: Kirankumar S
authors.initials: KS
authors.lastname: Mysore
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D018384:Oxidative Stress / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D013265:Stigmasterol / Q000378:metabolism*; D013696:Temperature
chemical_list: D029681:Arabidopsis Proteins; D003577:Cytochrome P-450 Enzyme System; D013265:Stigmasterol; C527746:CYP710A1 protein, Arabidopsis
keywords: cold tolerance; membrane stability; phytosterol; thermotolerance