title: Recovery and detection of botulinum neurotoxins from a nonporous surface.
abstract: We describe the adaptation of a sample recovery method for botulinum neurotoxins from stainless steel. Botulinum toxin was recovered from surfaces left to dry for up to 16 h and detected by either ELISA or EndoPep mass spectrometry methods. In addition, we demonstrate that this method can be used to evaluate the efficacy of surface decontamination procedures.
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Lautenschlager
authors.forename: Susan E
authors.initials: SE
authors.lastname: Maslanka
authors.forename: Paris A
authors.initials: PA
authors.lastname: Paul
authors.forename: Suzanne R
authors.initials: SR
authors.lastname: Kalb
authors.forename: John R
authors.initials: JR
authors.lastname: Barr
authors.forename: Brian H
authors.initials: BH
authors.lastname: Raphael
mesh_terms: D001905:Botulinum Toxins / Q000032:analysis* / Q000302:isolation & purification*; D004781:Environmental Exposure; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D013058:Mass Spectrometry; D013048:Specimen Handling / Q000379:methods*; D013193:Stainless Steel
chemical_list: D013193:Stainless Steel; D001905:Botulinum Toxins