title: The Vibrio harveyi bioassay used routinely to detect AI-2 quorum sensing inhibition is confounded by inconsistent normalization across marine matrices.
abstract: The Vibrio harveyi autoinducer-2 (AI-2) bioassay is used routinely to screen for inhibition of the AI-2 quorum sensing system. The present study utilizes three well-described bacterial strains to demonstrate that inconsistent normalization across matrices undermines the assay's use in screening marine samples for AI-2 inhibition.
authors.affiliation: Graduate Program in Marine Biology, College of Charleston, Charleston, SC 29412, USA.
authors.forename: Walter M
authors.initials: WM
authors.lastname: Blair
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Doucette
mesh_terms: D001681:Biological Assay / Q000379:methods* / Q000592:standards*; D006714:Homoserine / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors; D007783:Lactones / Q000037:antagonists & inhibitors*; D053038:Quorum Sensing; D014733:Vibrio / Q000187:drug effects* / Q000502:physiology*
chemical_list: D007783:Lactones; C091088:N-octanoylhomoserine lactone; D006714:Homoserine