title: Particulate matter promotes in vitro receptor-recognizable low-density lipoprotein oxidation and dysfunction of lipid receptors.
abstract: Particulate matter may promote cardiovascular disease, possibly as a consequence of its oxidative potential. Studies using susceptible animals indicate that particulate matter aggravates atherosclerosis by increasing lipid/macrophage content in plaques. Macrophage lipid uptake requires oxidized low-density lipoprotein and scavenger receptors; same receptors are involved in particulate matter uptake. We studied in vitro particulate matter potential to oxidize low-density lipoproteins and subsequent cell uptake through scavenger receptors. Particulate matter-induced low-density lipoproteins oxidation was evaluated by the thiobarbituric acid assay. Binding/internalization was tested in wild type and scavenger receptor-transfected Chinese hamster ovary cells, and in RAW264.7 cells using fluorescently labeled low-density lipoproteins. Dose-dependent binding/internalization only occurred in scavenger receptor-transfected Chinese hamster ovary cells and RAW264.7 cells. Competition binding/internalization using particles showed that particulate matter induced decreased binding (∼50%) and internalization (∼70%) of particle-oxidized low-density lipoproteins and native low-density lipoproteins. Results indicate that particulate matter was capable of oxidizing low-density lipoproteins, favoring macrophage internalization, and also altered scavenger and low-density lipoproteins receptor function.
authors.affiliation: Instituto Nacional de Cancerología, C.P. 14080 México, D.F., México.
authors.forename: Natalia
authors.initials: N
authors.lastname: Manzano-León
authors.forename: Jaime
authors.initials: J
authors.lastname: Mas-Oliva
authors.forename: Laura
authors.initials: L
authors.lastname: Sevilla-Tapia
authors.forename: Rocío
authors.initials: R
authors.lastname: Morales-Bárcenas
authors.forename: Jesús
authors.initials: J
authors.lastname: Serrano
authors.forename: Marie S
authors.initials: MS
authors.lastname: O Neill
authors.forename: Claudia M
authors.initials: CM
authors.lastname: García-Cuellar
authors.forename: Raúl
authors.initials: R
authors.lastname: Quintana
authors.forename: Inés
authors.initials: I
authors.lastname: Vázquez-López
authors.forename: Alvaro R
authors.initials: AR
authors.lastname: Osornio-Vargas
mesh_terms: D000818:Animals; D016466:CHO Cells / Q000187:drug effects / Q000378:metabolism; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D010084:Oxidation-Reduction; D052638:Particulate Matter / Q000378:metabolism* / Q000633:toxicity*; D011973:Receptors, LDL / Q000378:metabolism; D051116:Receptors, Scavenger / Q000378:metabolism
chemical_list: D008077:Lipoproteins, LDL; D052638:Particulate Matter; D011973:Receptors, LDL; D051116:Receptors, Scavenger; C099252:oxidized low density lipoprotein