title: Tetramolecular fluorescence complementation for detection of specific RNAs in vitro.
abstract: Short fuses: RNA can be detected sequence specifically by using two RNA binding proteins fused to short strands derived from GFP and an additional large GFP fragment. The tetramolecular system has a high signal-to-noise ratio, discriminates between closely related RNA, and works in RNA preparations as well as E. coli cell lysate.
authors.affiliation: University of Hamburg, Department of Chemistry, Institute of Biochemistry and Molecular Biology, Martin Luther King Platz 6, 20146 Hamburg, Germany.
authors.forename: Stefanie Julia
authors.initials: SJ
authors.lastname: Kellermann
authors.forename: Anna Katharina
authors.initials: AK
authors.lastname: Rath
authors.forename: Andrea
authors.initials: A
authors.lastname: Rentmeister
mesh_terms: D004926:Escherichia coli / Q000378:metabolism; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D012313:RNA / Q000032:analysis* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*
chemical_list: D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C465505:pumilio protein, human; D049452:Green Fluorescent Proteins; D012313:RNA