title: Optimization of cold-active lipase production from psychrophilic bacterium Moritella sp. 2-5-10-1 by statistical experimental methods.
abstract: Statistical experimental designs were applied to optimize cold-active lipase production by the psychrophilic bacterium Moritella sp. 2-5-10-1. First, a Plackett-Burmen design (PBD) was used to evaluate the significant effects of various fermentation parameters. The results indicated that soybean meal, temperature, and Tween-80 had significant influences on lipase production. The levels of these variables were optimized subsequently using central composite design (CCD). A quadratic regression model of cold-active lipase production was built, and verification experiments confirmed its validity. On subsequent scale-up in a 10-L bioreactor using optimized conditions, cold-active lipase production (30.56 U/mL) was obtained. The results clearly indicated that the model was adequate even on a large scale. To our knowledge, this is the first report of statistical optimization of cold-active lipase production by a psychrophilic bacterium.
authors.affiliation: School of Marine and Technology, Harbin Institute of Technology, Weihai, PR China.
authors.forename: Quanfu
authors.initials: Q
authors.lastname: Wang
authors.forename: Chunyu
authors.initials: C
authors.lastname: Zhang
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Hou
authors.forename: Xuezheng
authors.initials: X
authors.lastname: Lin
authors.forename: Jihong
authors.initials: J
authors.lastname: Shen
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Guan
mesh_terms: D000704:Analysis of Variance; D001426:Bacterial Proteins / Q000096:biosynthesis*; D019149:Bioreactors; D003080:Cold Temperature; D057075:Enzyme Assays; D005285:Fermentation; D008049:Lipase / Q000096:biosynthesis*; D015233:Models, Statistical; D034221:Moritella / Q000201:enzymology*; D011136:Polysorbates / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D011136:Polysorbates; D008049:Lipase