title: Rapid and semiautomated method for the analysis of veterinary drug residues in honey based on turbulent-flow liquid chromatography coupled to ultrahigh-performance liquid chromatography-Orbitrap mass spectrometry (TFC-UHPLC-Orbitrap-MS).
abstract: A simple and rapid method is described for the determination of veterinary drug (VD) residues in honey samples using turbulent flow chromatography coupled to ultrahigh-performance liquid chromatography-Orbitrap mass spectrometry (TFC-UHPLC-Orbitrap-MS). Honey samples were diluted with an aqueous solution of Na(2)EDTA (0.1 M). Then, they were injected into the chromatographic system including a TFC column. Afterward, the analytes were transferred to an UHPLC analytical column, where they were determined by UHPLC-Orbitrap-MS. Mean recoveries were obtained at three concentration levels (5, 10, and 50 μg/kg), ranging from 68 to 121% for most compounds. Repeatability (intraday precision) and interday precision (expressed as relative standard deviation, RSD) were <25% for most compounds. Limits of quantification (LOQs) ranged from 5 to 50 μg/kg and limits of identification (LOIs) from 0.1 to 50 μg/kg. The developed method was applied in honey samples, and it was fast and nonlaborious.
authors.affiliation: Department of Chemistry and Physics (Analytical Chemistry Area), Research Centre for Agricultural and Food Biotechnology (BITAL), University of Almerı́a, Agrifood Campus of International Excellence, Almerı́a, Spain.
authors.forename: María M
authors.initials: MM
authors.lastname: Aguilera-Luiz
authors.forename: Roberto
authors.initials: R
authors.lastname: Romero-González
authors.forename: Patricia
authors.initials: P
authors.lastname: Plaza-Bolaños
authors.forename: José L Martínez
authors.initials: JL
authors.lastname: Vidal
authors.forename: Antonia Garrido
authors.initials: AG
authors.lastname: Frenich
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D006722:Honey / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D019155:Veterinary Drugs / Q000032:analysis*
chemical_list: D019155:Veterinary Drugs