title: Immunoexpression of RANK, RANKL, OPG, VEGF, and vWF in radicular and dentigerous cysts.
abstract: BACKGROUND
  Radicular (RC) and dentigerous cysts (DC) can show a range from little to quite extensive primary/secondary inflammation and it is possible that the variation seen in the fibrous capsule of these cysts might reflect differences in the osteolytic activity. Moreover, the presence of hemorrhagic areas in the fibrous capsule of DC could also contribute to the increase in osteolytic activity. The aim of this study was to compare immunohistochemical expression of nuclear factor κappaB (RANK), RANK ligand (RANKL), and osteoprotegerin (OPG), vascular endothelial growth factor (VEGF) and angiogenic index in RC and DC.
  
  
  METHODS
  These proteins were evaluated in 20 RC and DC by immunohistochemistry. Angiogenic index was determined by microvessel count (MVC) using anti-von Willebrand factor antibody.
  
  
  RESULTS
  RANK and RANKL were higher in DC than RC in fibrous capsule. RC showed higher expression of VEGF in the epithelium and capsule. DC exhibited higher MVC than RC.
  
  
  CONCLUSIONS
  Ours results suggest that RANK and RANKL play an important role in bone resorption in DC and the hemorrhagic areas in the capsule of DC could be explained by increased vessel's number. The higher VEGF expression in RC might be related to nature of these lesions, where the inflammatory process contributes significantly to these findings.
authors.affiliation: Oral Pathology, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Maiara
authors.initials: M
authors.lastname: de Moraes
authors.forename: Felipe Rodrigues
authors.initials: FR
authors.lastname: de Matos
authors.forename: Lélia Batista
authors.initials: LB
authors.lastname: de Souza
authors.forename: Roseana
authors.initials: R
authors.lastname: de Almeida Freitas
authors.forename: Antônio
authors.initials: A
authors.lastname: de Lisboa Lopes Costa
mesh_terms: D001862:Bone Resorption / Q000473:pathology; D002452:Cell Count; D002467:Cell Nucleus / Q000473:pathology; D003238:Connective Tissue / Q000473:pathology; D003593:Cytoplasm / Q000473:pathology; D003803:Dentigerous Cyst / Q000473:pathology*; D004847:Epithelial Cells / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D055806:Microvessels / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000473:pathology; D053244:Osteoprotegerin / Q000032:analysis*; D053245:RANK Ligand / Q000032:analysis*; D011842:Radicular Cyst / Q000473:pathology*; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000032:analysis*; D017154:Stromal Cells / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis*; D014841:von Willebrand Factor / Q000032:analysis*
chemical_list: D053244:Osteoprotegerin; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; C505686:TNFRSF11A protein, human; C506030:TNFRSF11B protein, human; C505688:TNFSF11 protein, human; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D014841:von Willebrand Factor
keywords: angiogenesis; dentigerous cyst; odontogenic cyst; osteoclastogenesis; radicular cyst