title: A Toll-like receptor 3 agonist (polyI:C) elicits innate host responses in the spleen and lungs of chickens.
abstract: Double-stranded (ds) RNA interacts with host Toll-like receptor (TLR-3), leading to the induction of anti-viral host responses. The present study was designed to compare different routes of administration of a synthetic dsRNA (polyI:C) for induction of innate responses in chicken spleen and lungs. Chickens were treated with polyI:C via the aerosol, intra-air sac (i.a.s.), and intramuscular (IM) routes. Spleen and lungs were collected at 0, 2, 6, 12, and 24 h post-treatment and the expression of innate defence genes was quantified by real-time reverse transcriptase polymerase chain reaction (RT-PCR). There was an up-regulation of interferon (IFN)-β, TLR-3, and Toll/interleukin 1 receptor domain-containing adaptor protein inducing IFN-β (TRIF) at 6 h post-treatment in the spleen via IM administration of polyI:C. There was also an increase in the expression of TLR-3 and TRIF in the spleen at 2 h post-treatment via the i.a.s. route. The expression of IFN-α and TRIF was upregulated at 6 h post-treatment via the i.a.s. route in the lungs. Overall, our results indicate that administration of polyI:C can locally and systemically induce the expression of innate response genes depending on the route.
authors.affiliation: Department of Pathobiology, University of Guelph, Guelph, Ontario.
authors.forename: Payvand
authors.initials: P
authors.lastname: Parvizi
authors.forename: Amirul Islam
authors.initials: AI
authors.lastname: Mallick
authors.forename: Kamran
authors.initials: K
authors.lastname: Haq
authors.forename: Benjamin
authors.initials: B
authors.lastname: Schlegel
authors.forename: Shayan
authors.initials: S
authors.lastname: Sharif
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002645:Chickens; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D007113:Immunity, Innate / Q000187:drug effects*; D016899:Interferon-beta / Q000235:genetics / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D011070:Poly I-C / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects* / Q000378:metabolism; D051196:Toll-Like Receptor 3 / Q000819:agonists* / Q000235:genetics / Q000378:metabolism*
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D051196:Toll-Like Receptor 3; D016899:Interferon-beta; D011070:Poly I-C