title: Dynamic distribution of HDAC1 and HDAC2 during mitosis: association with F-actin.
abstract: During mitosis, histone deacetylase 2 (HDAC2) becomes highly phosphorylated through the action of CK2, and HDAC1 and 2 are displaced from mitotic chromosomes. HDAC1 and 2 are components of corepressor complexes, which function with lysine acetyltransferases to catalyze dynamic protein acetylation and regulate gene expression. In this study, we show that HDAC1 and 2 associate with F-actin in mitotic cells. Inhibition of Aurora B or protein kinase CK2 did not prevent the displacement of HDAC1 and 2 from mitotic chromosomes in HeLa cells. Further, proteins of the HDAC1 and 2 corepressor complexes and transcription factors recruiting these corepressors to chromatin were dissociated from mitotic chromosomes independent of Aurora B activity. HDAC1 and 2 returned to the nuclei of daughter cells during lamin A/C reassembly and before Sp1, Sp3, and RNA polymerase II. Our results show that HDAC1 and 2 corepressor complexes are removed from the mitotic chromosomes and are available early in the events leading to the re-establishment of the gene expression program in daughter cells.
authors.affiliation: Manitoba Institute of Child Health, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Shihua
authors.initials: S
authors.lastname: He
authors.forename: Dilshad H
authors.initials: DH
authors.lastname: Khan
authors.forename: Stefan
authors.initials: S
authors.lastname: Winter
authors.forename: Christian
authors.initials: C
authors.lastname: Seiser
authors.forename: James R
authors.initials: JR
authors.lastname: Davie
mesh_terms: D000199:Actins / Q000378:metabolism*; D064107:Aurora Kinase B; D064127:Aurora Kinases; D002877:Chromosomes, Human / Q000378:metabolism; D044127:Epigenesis, Genetic; D006367:HeLa Cells; D056284:Histone Deacetylase 1 / Q000378:metabolism*; D056464:Histone Deacetylase 2 / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D061986:MCF-7 Cells; D008938:Mitosis / Q000235:genetics / Q000502:physiology*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism
chemical_list: D000199:Actins; D006657:Histones; C576921:AURKB protein, human; D064107:Aurora Kinase B; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases; C110550:HDAC1 protein, human; C533423:HDAC2 protein, human; D056284:Histone Deacetylase 1; D056464:Histone Deacetylase 2