title: Real-time multiplex PCR assay and melting curve analysis for identifying diarrheagenic Escherichia coli.
abstract: A real-time multiplex PCR assay was designed to amplify the virulence genes eae, pEAF, aatA, daaC, elt, est, ipaH, stx(1), and stx(2) for the detection of all diarrheagenic Escherichia coli pathotypes. This assay proved to be more sensitive and rapid than a conventional multiplex PCR for diarrheagenic E. coli isolates from children with diarrhea.
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Tamara B
authors.initials: TB
authors.lastname: Souza
authors.forename: Diego M
authors.initials: DM
authors.lastname: Lozer
authors.forename: Sônia M S
authors.initials: SM
authors.lastname: Kitagawa
authors.forename: Liliana C
authors.initials: LC
authors.lastname: Spano
authors.forename: Neusa P
authors.initials: NP
authors.lastname: Silva
authors.forename: Isabel C A
authors.initials: IC
authors.lastname: Scaletsky
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D002648:Child; D003967:Diarrhea / Q000175:diagnosis / Q000382:microbiology; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000175:diagnosis / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics; D005838:Genotype; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D044366:Transition Temperature; D037521:Virulence Factors / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; D037521:Virulence Factors