title: Quantification of cell cycle-arresting proteins.
abstract: Cellular senescence, which can be defined as a stress response preventing the propagation of cells that have accumulated potentially oncogenic alterations, is invariably associated with a permanent cell cycle arrest. Such an irreversible blockage is mainly mediated by the persistent upregulation of one or more cyclin-dependent kinase inhibitors (CKIs), including (though not limited to) p16( INK4A ) and p21( CIP1 ) and p27( KIP1 ). CKIs operate by binding to cyclin-dependent kinases (CDKs), de facto inhibiting their enzymatic activity. Here, we provide an immunoblotting-based method for the detection and quantification of CKIs in vitro and ex vivo, together with a set of guidelines for the interpretation of results.
authors.affiliation: INSERM, U848, Villejuif, France.
authors.forename: Oliver
authors.initials: O
authors.lastname: Kepp
authors.forename: Isabelle
authors.initials: I
authors.lastname: Martins
authors.forename: Laurie
authors.initials: L
authors.lastname: Menger
authors.forename: Mickaël
authors.initials: M
authors.lastname: Michaud
authors.forename: Sandy
authors.initials: S
authors.lastname: Adjemian
authors.forename: Abdul Qader
authors.initials: AQ
authors.lastname: Sukkurwala
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Galluzzi
authors.forename: Guido
authors.initials: G
authors.lastname: Kroemer
mesh_terms: D059447:Cell Cycle Checkpoints; D016922:Cellular Senescence; D050756:Cyclin-Dependent Kinase Inhibitor Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004586:Electrophoresis; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting / Q000379:methods*
chemical_list: D050756:Cyclin-Dependent Kinase Inhibitor Proteins