title: Screening for cytotoxic activity of ovotransferrin and its enzyme hydrolysates.
abstract: The aim of this study was to investigate the cytotoxic activities of ovotransferrin (OTF) from egg white and its enzyme hydrolysates (OTH). The OTF was hydrolyzed at 45°C for 3 h using neutrase, alcalase, acid (0.03 N HCl, pH 2.5), protamex, protex 6L, flavorzyme, α-chymotrypsin, trypsin, and collupulin MG. The enzyme to substrate ratio was 1:25 (wt/wt) in all experiments. Using the 3-(4,5-dimethylthizol-2-yl)-2,5-diphenylatetetrazolium bromide (MTT) assay, the cytotoxicity of OTF and OTH was evaluated in human cancer cell lines of various tissue origins, including the lung (A549 and SK-MES-1), stomach (AGS), breast (MCF-7), larynx (Hep-2), cervix (HeLa), and liver (HepG2). The growth of all cancer cell lines was inhibited by both OTF and OTH in a dose-dependent manner. In particular, OTF displayed relatively high cytotoxicity (≤60% inhibition effects) at 40 mg/mL. At lower concentrations (≤5 mg/mL), however, OTF- and OTH-mediated cytotoxic effects were not significant in all cancer cell lines tested. The MCF-7 cells were the least sensitive to all treatments among all cancer cell lines tested. The OTH-trypsin and OTH-neutrase showed a potent cytotoxicity (over 90% cytotoxicity) to HeLa cells at the 10 mg/mL level. The OTH-trypsin, OTH-protamex, OTH-protex 6L, and OTH-collupulin MG caused 95, 96, 86, and 87% growth inhibition, respectively, in AGS cells. These results indicated there are possibilities that OTF and OTH can be used as natural growth inhibitors of human cancer cell lines.
authors.affiliation: Division of Animal Life Science and Bio/Molecular Informatics Center, Konkuk University, Seoul 143-701, Korea.
authors.forename: S H
authors.initials: SH
authors.lastname: Moon
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
authors.forename: Y J
authors.initials: YJ
authors.lastname: Lee
authors.forename: K H
authors.initials: KH
authors.lastname: Chang
authors.forename: J Y
authors.initials: JY
authors.lastname: Paik
authors.forename: D U
authors.initials: DU
authors.lastname: Ahn
authors.forename: H D
authors.initials: HD
authors.lastname: Paik
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens; D003207:Conalbumin / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004529:Egg White / Q000737:chemistry; D006801:Humans; D013778:Tetrazolium Salts / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; D003207:Conalbumin; C022616:thiazolyl blue