title: [Endophyte microbial community in Achnatherum inebrians].
abstract: OBJECTIVE
  The aim of this study was to investigate the composition and distribution variation of endophytic bacteria and fungi in Achnatherum inebrians.
  
  
  METHODS
  The DNA of seed, leaf, stem and root was extracted with liquid nitrogen grinding method. Then, 16S rDNA and Internally Transcribed Spacer (ITS) sequence were digested with restriction enzymes Hha I , Rsa I and Hhae III, Hinf I to obtain terminal restriction fragments. The terminal restriction fragments were matched to bacterial and fungal genera by the T-RFLP Analysis Program, and the community component and similarity of endophyte in Achnatherum inebrians were analyzed.
  
  
  RESULTS
  The diversity of endophytic bacteria and fungi was the most abundant in root and seed of Achnatherum inebrians. All the predominant bacterial population was Bacillus (above 29%) in different organs of Achnatherum inebrians. The predominant fungal population was Mycosphaerella (6.5%), Teratosphaeria (4.5%), Fragum (1.1%), Sebacina (11.3%) in seed, leaf, stem and root, respectively. The structure of the bacterial communities in the stem and leaf were similar, whereas the structure of the bacterial communities in the seed and other tissue were different. The structure of the fungi communities in the stem and seed were similar, whereas the structure of the fungi communities in the leaf and other tissue were different.
  
  
  CONCLUSIONS
  There was abundant endophytic microbial diversity in Achnatherum inebrians.
authors.affiliation: Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China. syw1973@126.com
authors.forename: Yingwu
authors.initials: Y
authors.lastname: Shi
authors.forename: Xuebing
authors.initials: X
authors.lastname: Zhang
authors.forename: Kai
authors.initials: K
authors.lastname: Lou
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D060026:Endophytes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D018547:Plant Stems / Q000382:microbiology; D006109:Poaceae / Q000382:microbiology* / Q000502:physiology; D012150:Polymorphism, Restriction Fragment Length