title: [Typing and identification of non-polio enterovirus from acute flaccid paralysis cases in Ningxia, 1997-2011].
abstract: OBJECTIVE
  To identify the serotype of 73 non-polio enterovirus (NPEV) strains from acute flaccid paralysis (AFP) cases in Ningxia province, during 1997 - 2011.
  
  
  METHODS
  Partial sequencing of the VP1 region was amplified by RT-PCR with degenerate primers and sequenced while sequences were compared with the database of GenBank by the BLAST algorithm. Evolution was analyzed by constructing phylogenetic tree using Mega 5.1.
  
  
  RESULTS
  In this study, a total of 73 NPEVs were analyzed, including 4 strains un-typed, 69 strains typed by RT-PCR. A total of 27 serotypes were identified, including 8 serotypes of human enterovirus (HEV)-A, 19 serotypes of HEV-B. The HEV-B group (46/69, 66.7%) constituted the largest proportion of isolates, followed by HEV-A (23/69, 33.3%), but no strains were found that belonged to HEV-C or HEV-D group. In the 69 strains, enterovirus 71 was the most frequently seen isolates, followed by coxsackie-virus A4, 16, 9 and echovirus 24, 6.
  
  
  CONCLUSIONS
  HEV-B was the most predominant (46/69, 66.7%) serotype of NPEV in Ningxia during the AFP surveillance, in 1997 - 2011.
authors.affiliation: Ningxia Center for Disease Control and Prevention, Yinchuan, China.
authors.forename: Jiang-tao
authors.initials: JT
authors.lastname: Ma
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Fang
authors.initials: F
authors.lastname: Yuan
authors.forename: Xue-min
authors.initials: XM
authors.lastname: Ma
authors.forename: Guang-yu
authors.initials: GY
authors.lastname: Guan
authors.forename: Jun
authors.initials: J
authors.lastname: Zhan
mesh_terms: D002681:China / Q000453:epidemiology; D017931:DNA Primers; D004770:Enterovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005243:Feces / Q000821:virology; D006801:Humans; D010802:Phylogeny; D011051:Poliomyelitis / Q000453:epidemiology / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics
chemical_list: D017931:DNA Primers; D012367:RNA, Viral