title: Isobutanol production from D-xylose by recombinant Saccharomyces cerevisiae.
abstract: Simultaneous overexpression of an optimized, cytosolically localized valine biosynthesis pathway together with overexpression of xylose isomerase XylA from Clostridium phytofermentans, transaldolase Tal1 and xylulokinase Xks1 enabled recombinant Saccharomyces cerevisiae cells to complement the valine auxotrophy of ilv2,3,5 triple deletion mutants for growth on D-xylose as the sole carbon source. Moreover, after additional overexpression of ketoacid decarboxylase Aro10 and alcohol dehydrogenase Adh2, the cells were able to ferment D-xylose directly to isobutanol.
authors.affiliation: Institute of Molecular Biosciences, Goethe-University Frankfurt, Frankfurt am Main, Germany.
authors.forename: Dawid
authors.initials: D
authors.lastname: Brat
authors.forename: Eckhard
authors.initials: E
authors.lastname: Boles
mesh_terms: D000440:Butanols / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D003013:Clostridium / Q000201:enzymology / Q000235:genetics; D017353:Gene Deletion; D015870:Gene Expression; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*
chemical_list: D000440:Butanols; D011994:Recombinant Proteins; C040507:isobutyl alcohol; D002244:Carbon; D014994:Xylose