title: Jerusalem artichoke powder: a useful material in producing high-optical-purity l-lactate using an efficient sugar-utilizing thermophilic Bacillus coagulans strain.
abstract: Jerusalem artichoke is a low-requirement crop, which does not interfere with food chain, and is a promising carbon source for industrial fermentation. Microbial conversion of such a renewable raw material to useful products, such as lactic acid, is an important objective in industrial biotechnology. In this study, high-optical-purity l-lactate was efficiently produced from the hydrolysates of Jerusalem artichoke powder by a thermophilic bacterium, Bacillus coagulans XZL4. High l-lactate production (134gl(-1)) was obtained using 267gl(-1) Jerusalem artichoke powder (total reducing sugars of 140gl(-1)) and 10gl(-1) of corn steep powder in fed-batch fermentation, with an average productivity of 2.5gl(-1)h(-1) and a yield of 0.96gg(-1) reducing sugars. The final product optical purity is 99%, which meets the requirement of lactic acid polymerization. Our study represents a cost-effective and promising method for polymer-grade l-lactate production using a cheap raw bio-resource.
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
authors.forename: Limin
authors.initials: L
authors.lastname: Wang
authors.forename: Zhangwei
authors.initials: Z
authors.lastname: Xue
authors.forename: Bo
authors.initials: B
authors.lastname: Zhao
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
authors.forename: Yanhe
authors.initials: Y
authors.lastname: Ma
mesh_terms: D001407:Bacillus / Q000378:metabolism*; D005285:Fermentation; D006368:Helianthus / Q000737:chemistry* / Q000378:metabolism; D006868:Hydrolysis; D019344:Lactic Acid / Q000302:isolation & purification* / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D003313:Zea mays
chemical_list: D019344:Lactic Acid; D009584:Nitrogen