title: Bioconversion of sawdust into ethanol using dilute sulfuric acid-assisted continuous twin screw-driven reactor pretreatment and fed-batch simultaneous saccharification and fermentation.
abstract: Ethanol production from poplar sawdust using sulfuric acid-assisted continuous twin screw-driven reactor (CTSR) pretreatment followed by simultaneous saccharification and fermentation (SSF) was investigated. Pretreatment with high acid concentration increased the cellulose content in the pretreated solid (74.9-76.9% in the range of 4.0-5.5wt.% H(2)SO(4)). The sugar content (XMG; xylan+mannan+galactan) in the treated-solid was 11.1-15.2% and 0.9-5.7% with 0.5wt.% and 7.0wt.%, respectively. The XMG recovery yield of the sample treated with 4.0wt.% H(2)SO(4) at 185°C was maximized at 88.6%. Enzymatic hydrolysis test showed a cellulose digestibility of 67.1%, 70.1%, and 73.6% with 15, 30, and 45FPU/g-cellulose, respectively. In the fed-batch SSF tests with initial enzyme addition, the ethanol yield of each stage almost reached a maximum at 28h, 48h, and 56h, respectively, with yields of 63.9% (16.5g/L), 78.4% (30.1g/L), and 81.7% (39.9g/L), respectively.
authors.affiliation: Department of Environmental Engineering, Kongju National University, Cheonan, Chungnam 330-717, Republic of Korea.
authors.forename: Tae Hyun
authors.initials: TH
authors.lastname: Kim
authors.forename: Chang Ho
authors.initials: CH
authors.lastname: Choi
authors.forename: Kyeong Keun
authors.initials: KK
authors.lastname: Oh
mesh_terms: D061249:Batch Cell Culture Techniques; D056804:Biofuels; D019149:Bioreactors; D002241:Carbohydrates / Q000302:isolation & purification; D000431:Ethanol / Q000302:isolation & purification / Q000378:metabolism*; D005285:Fermentation; D032107:Populus / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013464:Sulfuric Acids / Q000378:metabolism*; D013696:Temperature; D014934:Wood / Q000378:metabolism
chemical_list: D056804:Biofuels; D002241:Carbohydrates; D013464:Sulfuric Acids; D000431:Ethanol; C033158:sulfuric acid