title: Prevalence of yeast other than Candida albicans in denture wearers.
abstract: OBJECTIVE
  The isolation of yeast species other than Candida albicans from the oral mucosa has been increasing in frequency, suggesting that those may constitute emerging potential oral colonizers. The purpose of this work was to determine whether yeast species other than C. albicans are associated with factors related to wearing of dental prostheses.
  
  
  METHODS
  tRNA-PCR fingerprinting and sequencing of the 26S rDNA D1/D2 domain were used to identify all yeasts isolated from CHROMagar™ Candida cultures of oral swabs collected from 178 patients.
  
  
  RESULTS
  Besides C. albicans, 13 other species were identified, corresponding to 34% of the yeast isolates. The majority of the non-C. albicans species were not detected as single colonizers but rather in co-colonization with one or two other yeasts, often with C. albicans. No significant associations were found with non-C. albicans species. On the contrary, the best-fitted logistic regression model predicts that either wearing a denture (adjusted odds = 4.6) or insufficient oral hygiene (adjusted odds = 2.3) are risks for colonization by yeast, in general.
  
  
  CONCLUSIONS
  The colonization with non-C. albicans species and co-colonization were not independently associated with any of the analyzed host-related factors. In particular, neither wearing a removable denture nor being elderly were significant predictors.
authors.affiliation: Instituto Superior Ciências da Saúde Egas Moniz, Centro de Investigação Interdisciplinar Egas Moniz, Caparica, Portugal.
authors.forename: Inês
authors.initials: I
authors.lastname: Cavaleiro
authors.forename: Luis
authors.initials: L
authors.lastname: Proença
authors.forename: Sérgio
authors.initials: S
authors.lastname: Félix
authors.forename: Madalena
authors.initials: M
authors.lastname: Salema-Oom
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000935:Antifungal Agents / Q000494:pharmacology; D002175:Candida / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification; D002176:Candida albicans / Q000187:drug effects / Q000302:isolation & purification; D041221:Candida glabrata / Q000187:drug effects / Q000302:isolation & purification; D041022:Candida tropicalis / Q000187:drug effects / Q000302:isolation & purification; D016172:DNA Fingerprinting; D003778:Dentures / Q000382:microbiology*; D005260:Female; D015725:Fluconazole / Q000494:pharmacology; D006801:Humans; D008297:Male; D056265:Microbial Interactions; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D009061:Mouth Mucosa / Q000382:microbiology; D009910:Oral Hygiene / Q000706:statistics & numerical data; D016133:Polymerase Chain Reaction / Q000379:methods; D011446:Prospective Studies; D011743:Pyrimidines / Q000494:pharmacology; D012331:RNA, Fungal / Q000032:analysis; D012335:RNA, Ribosomal / Q000032:analysis; D012343:RNA, Transfer / Q000032:analysis; D014230:Triazoles / Q000494:pharmacology; D065819:Voriconazole
chemical_list: D000935:Antifungal Agents; D011743:Pyrimidines; D012331:RNA, Fungal; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S; D014230:Triazoles; D015725:Fluconazole; D012343:RNA, Transfer; D065819:Voriconazole
keywords: Oral colonization; dentures; non-Candida albicans spp; yeast