title: Passive experimental autoimmune encephalomyelitis in C57BL/6 with MOG: evidence of involvement of B cells.
abstract: Experimental autoimmune encephalomyelitis (EAE) is the most relevant animal model to study demyelinating diseases such as multiple sclerosis. EAE can be induced by active (active EAE) or passive (at-EAE) transfer of activated T cells in several species and strains of rodents. However, histological features of at-EAE model in C57BL/6 are poorly described. The aim of this study was to characterize the neuroinflammatory and neurodegenerative responses of at-EAE in C57BL/6 mice by histological techniques and compare them with that observed in the active EAE model. To develop the at-EAE, splenocytes from active EAE female mice were harvested and cultured in presence of MOG(35-55) and IL-12, and then injected intraperitoneally in recipient female C57BL6/J mice. In both models, the development of EAE was similar except for starting before the onset of symptoms and presenting a higher EAE cumulative score in the at-EAE model. Spinal cord histological examination revealed an increased glial activation as well as more extensive demyelinating areas in the at-EAE than in the active EAE model. Although inflammatory infiltrates composed by macrophages and T lymphocytes were found in the spinal cord and brain of both models, B lymphocytes were significantly increased in the at-EAE model. The co-localization of these B cells with IgG and their predominant distribution in areas of demyelination would suggest that IgG-secreting B cells are involved in the neurodegenerative processes associated with at-EAE.
authors.affiliation: Center for Neuroimmunology, Service of Neurology, Hospital Clinic and Institut d'Investigació Biomèdica August Pi I Sunyer (IDIBAPS), Barcelona, Spain.
authors.forename: Francesco
authors.initials: F
authors.lastname: Mannara
authors.forename: Tony
authors.initials: T
authors.lastname: Valente
authors.forename: Josep
authors.initials: J
authors.lastname: Saura
authors.forename: Francesc
authors.initials: F
authors.lastname: Graus
authors.forename: Albert
authors.initials: A
authors.lastname: Saiz
authors.forename: Beatriz
authors.initials: B
authors.lastname: Moreno
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001921:Brain / Q000276:immunology; D003711:Demyelinating Diseases / Q000150:complications; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000150:complications / Q000276:immunology* / Q000473:pathology; D005260:Female; D007116:Immunization, Passive; D051379:Mice; D008810:Mice, Inbred C57BL; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000276:immunology*; D009457:Neuroglia / Q000276:immunology / Q000473:pathology; D009474:Neurons / Q000473:pathology; D010446:Peptide Fragments / Q000276:immunology; D013116:Spinal Cord / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; C526792:myelin oligodendrocyte glycoprotein (35-50), Ala(37)-