title: NTA-modified carbon electrode as a general relaying substrate to facilitate electron transfer of SOD: application to in vivo monitoring of O2(-) in a rat brain.
abstract: Nitrilotriacetic acid (NTA)/histidine-tag (HT) technology has been first employed to facilitate the electron transfer of superoxide dismutase (SOD), and further been developed for in vivo monitoring of superoxide anion (O2(-)) in a rat brain during cerebral ischemia/reperfusion processes. Direct electron transfer of SOD is greatly enhanced at NTA-modified electrode with a high rate constant (ks) of 24±1.1s(-1). The formal potential (E(0)') of SOD is estimated to be 5±2.5mV vs. Ag
authors.affiliation: State Key Laboratory of Pollution Control and Resources Reuse, Department of Chemistry, Tongji University, Siping Road 1239, Shanghai 200092, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Di
authors.initials: D
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Gu
authors.forename: Anwei
authors.initials: A
authors.lastname: Zhu
authors.forename: Yang
authors.initials: Y
authors.lastname: Tian
authors.forename: Guoyue
authors.initials: G
authors.lastname: Shi
mesh_terms: D000085:Acetates / Q000737:chemistry*; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation; D001921:Brain / Q000378:metabolism*; D002244:Carbon / Q000737:chemistry; D003217:Conductometry / Q000295:instrumentation*; D004566:Electrodes; D004579:Electron Transport; D004867:Equipment Design; D019544:Equipment Failure Analysis; D010100:Oxygen / Q000032:analysis*; D051381:Rats; D015427:Reperfusion Injury / Q000175:diagnosis / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000737:chemistry*
chemical_list: D000085:Acetates; C028257:nitroacetic acid; D002244:Carbon; D013482:Superoxide Dismutase; D010100:Oxygen