title: The effects of Mn(2+) on the proliferation, osteogenic differentiation and adipogenic differentiation of primary mouse bone marrow stromal cells.
abstract: The effects of Mn(2+) on the proliferation, osteogenic and adipogenic differentiation of BMSCs were evaluated by employing MTT, ΔΨm, cell cycle, ALP activity, collagen production, ARS and oil red O stain assays. The results indicated that Mn(2+) decreased the viability at most concentrations for 24 h, but the viability was increased with prolonging incubation time. Mn(2+) at the concentrations of 1×10(-7) and 1×10(-6)mol/L decreased ΔΨm in the BMSCs for 48 h. Mn(2+) induced G2/M phase cell cycle arrest at tested concentrations. On day 7 and 10, the effect of Mn(2+) on the osteogenic differentiation depended on concentration, but it inhibited osteogenic differentiation at all tested concentrations for 14 d. The effect of Mn(2+) on the synthesis of collagen of BMSCs depended on concentration for 7 d, but Mn(2+) inhibited the synthesis of collagen at all tested concentrations for 10 d. On day 14, Mn(2+) inhibited the formation of mineralized matrix nodules of BMSCs at all tested concentrations, the inhibitory effect turned to be weaker with prolonging incubation time. Mn(2+) promoted the adipogenic differentiation of BMSCs at all tested concentrations for 10 d, but had no effect with prolonging incubation time. These findings suggested the effects of Mn(2+) on the proliferation, osteogenic differentiation and adipogenic differentiation of BMSCs are very complicated, concentration and incubation time are key factors for switching the biological effects of Mn(2+) from damage to protection.
authors.affiliation: College of Chemistry and Environmental Science, Chemical Biology Key Laboratory of Hebei Province, Hebei University, Baoding, People's Republic of China. jczhang6970@yahoo.com.cn
authors.forename: Jinchao
authors.initials: J
authors.lastname: Zhang
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhang
authors.forename: Shenghui
authors.initials: S
authors.lastname: Li
authors.forename: Yingjian
authors.initials: Y
authors.lastname: Hou
authors.forename: Haisong
authors.initials: H
authors.lastname: Zhang
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D008345:Manganese / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D010012:Osteogenesis / Q000187:drug effects*; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects; D013329:Structure-Activity Relationship
chemical_list: D008345:Manganese