title: Isolation and characterization of an anti-insect β-toxin from the venom of the scorpion Isometrus maculatus.
abstract: Im-3 was isolated from the venom of the scorpion Isometrus maculatus through several steps of HPLC fractionation based on the insect paralytic activity. Injecting Im-3 into crickets induced paralysis, but no toxicity was apparent in mice after an intracerebroventricular injection. Im-3 shares sequence similarity to scorpion β-toxins that specifically affect insect sodium channels.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Kawachi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Miyashita
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: Hisashi
authors.initials: H
authors.lastname: Miyagawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001180:Arthropod Venoms / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D006135:Gryllidae / Q000187:drug effects* / Q000502:physiology; D007276:Injections, Intraventricular; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification / Q000494:pharmacology; D010802:Phylogeny; D012605:Scorpions / Q000737:chemistry* / Q000472:pathogenicity; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D026941:Sodium Channel Blockers / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification / Q000494:pharmacology; D015222:Sodium Channels / Q000378:metabolism; D013045:Species Specificity
chemical_list: D001180:Arthropod Venoms; D009498:Neurotoxins; D026941:Sodium Channel Blockers; D015222:Sodium Channels