title: The cellular biology of tendon grafting.
abstract: We investigated the cellular biology of tendon grafting in a mouse model using green fluorescent protein mismatch grafting and quantitative immunohistochemistry of molecular markers for inflammation, proliferation, collagen synthesis, cell death, and myofibroblast/pericyte expression. We provide a detailed analysis of the healing characteristics during the phases of inflammation, synthesis, and remodelling. Our findings indicated that survival of the cells in the grafted tendon was finite. Syngenic and autologous grafts provoked a similar cellular reaction and all grafts healed. Cells in the graft contributed significantly to collagen synthesis and do have a role in healing.
authors.affiliation: Plastic Surgery Research, Inflammation Sciences, School of Translational Medicine, University of Manchester, Manchester, UK.
authors.forename: N
authors.initials: N
authors.lastname: Alam
authors.forename: D A
authors.initials: DA
authors.lastname: McGrouther
authors.forename: J K F
authors.initials: JK
authors.lastname: Wong
mesh_terms: D000125:Achilles Tendon / Q000601:surgery; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D064592:Autografts; D049452:Green Fluorescent Proteins; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D013710:Tendons / Q000637:transplantation*; D014945:Wound Healing / Q000502:physiology
chemical_list: D049452:Green Fluorescent Proteins
keywords: Tendon; apoptosis; collagen synthesis; grafting; inflammation; mouse; proliferation; vascularization