title: A validated high-performance liquid chromatography method with diode array detection for simultaneous determination of nine flavonoids in Senecio cannabifolius Less.
abstract: A reversed phase high performance liquid chromatography method coupled with a diode array detector (HPLC-DAD) was developed for the first time for the simultaneous determination of 9 flavonoids in Senecio cannabifolius, a traditional Chinese medicinal herb. Agilent Zorbax SB-C18 column was used at room temperature and the mobile phase was a mixture of acetonitrile and 0.5% formic acid (v/v) in water in the gradient elution mode at a flow-rate of 1.0mlmin(-1), detected at 360nm. Validation of this method was performed to verify the linearity, precision, limits of detection and quantification, intra- and inter-day variabilities, reproducibility and recovery. The calibration curves showed good linearities (R(2)>0.9995) within the test ranges. The relative standard deviation (RSD) of the method was less than 3.0% for intra- and inter-day assays. The samples were stable for at least 96h, and the average recoveries were between 90.6% and 102.5%. High sensitivity was demonstrated with detection limits of 0.028-0.085μg/ml for flavonoids. The newly established HPLC method represents a powerful technique for the quality assurance of S. cannabifolius.
authors.affiliation: National Engineering Laboratory for Durggable Gene and Protein Screening, Northeast Normal University, Changchun 130024, China.
authors.forename: Tian-Zeng
authors.initials: TZ
authors.lastname: Niu
authors.forename: Yu-Wei
authors.initials: YW
authors.lastname: Zhang
authors.forename: Yong-Li
authors.initials: YL
authors.lastname: Bao
authors.forename: Yin
authors.initials: Y
authors.lastname: Wu
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Yu
authors.forename: Lu-Guo
authors.initials: LG
authors.lastname: Sun
authors.forename: Jing-Wen
authors.initials: JW
authors.lastname: Yi
authors.forename: Yan-Xin
authors.initials: YX
authors.lastname: Huang
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Li
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis; D005419:Flavonoids / Q000032:analysis* / Q000302:isolation & purification; D057230:Limit of Detection; D011786:Quality Control; D015203:Reproducibility of Results; D012674:Senecio / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids