title: Expression patterns of As-ClC gene of Artemia sinica in early development and under salinity stress.
abstract: As-ClC (chloride channels protein from Artemia sinica), a member from the chloride channels protein family, is a α-helical membrane protein predicted to traverse the cell membrane 11 times. It is important for several physiological functions such as cell volume regulation, cell proliferation, growth and differentiation. In this paper, the complete cDNA sequence of As-CIC was cloned from A. sinica for the first time using RACE technology. The expression pattern and location of the As-CIC gene was investigated in different stages of the embryonic development by means of quantitative real-time PCR and in situ hybridization (ISH) assay. As-CLC was distributed throughout the whole body in cells of different embryonic development of A. sinica as shown by ISH. There was a low expression level of the As-ClC gene after 0 h and a higher expression level after 15 and 40 h when the embryo entered the next growth period and the environmental salinity changed. At adult stage, the As-ClC maintained a high expression level. The results of the real-time PCR assay showed an increasing trend of As-ClC transcripts with increasing salinity. The expression of As-ClC was higher in the control group (28) than in the experimental group except at a salinity of 200 PSU. It indicated that As-ClC functions as salinity-stress-related gene, probably participated in cell volume regulation and osmotic regulation during the early embryonic development of A. sinica.
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116029, People's Republic of China.
authors.forename: Qiaozhi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Hou
authors.forename: Qiuying
authors.initials: Q
authors.lastname: Li
authors.forename: Lulu
authors.initials: L
authors.lastname: Han
authors.forename: Zhe
authors.initials: Z
authors.lastname: Yuan
authors.forename: Jian
authors.initials: J
authors.lastname: Tan
authors.forename: Bin
authors.initials: B
authors.lastname: Du
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Zou
authors.forename: Lin
authors.initials: L
authors.lastname: Hou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia / Q000196:embryology* / Q000502:physiology*; D001483:Base Sequence; D003001:Cloning, Molecular; D047108:Embryonic Development / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D011487:Protein Conformation; D054712:Salinity; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015222:Sodium Channels / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D013312:Stress, Physiological
chemical_list: D015222:Sodium Channels