title: Separation of the antioxidant compound quercitrin from Lindera obtusiloba Blume and its antimelanogenic effect on B16F10 melanoma cells.
abstract: Considering the growing evidence of the presence of antioxidant compounds in plant extracts, the objectives of this study were to identify antioxidant compounds in Lindera obtusiloba Blume (Lauraceae) and to evaluate their antimelanogenic activities on B16F10 melanoma cells. Organic solvent fractions were separated from L. obtusiloba extracts (LOE). The ethyl acetate fraction (LOE-E) was significantly active against oxidative damage induced by tert-butyl hydroperoxide in primary rat hepatocytes. Two single purified compounds, quercitrin (quercetin-3-O-α-L-rhamnopyranoside) and afzelin (kaempferol-3-O-α-L-rhamnoside), were identified by HPLC and NMR. These compounds were evaluated for antioxidant activities by 1,1-diphenyl 2-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP) assay, and for their antimelanogenic activities by tyrosinase inhibitory assay melanin formation inhibition assay and Western bolt analysis for the signaling pathway. The significant effects of quercitrin on antioxidant and antimelanogenic activities, and signal modulation of ERK and MITF in B16F10 melanoma cells were observed. This is the first report to identify quercitrin in L. obtusiloba and its whitening effect.
authors.affiliation: Division of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University, Seoul, South Korea.
authors.forename: Chung-Oui
authors.initials: CO
authors.lastname: Hong
authors.forename: Hyun Ah
authors.initials: HA
authors.lastname: Lee
authors.forename: Chae Hong
authors.initials: CH
authors.lastname: Rhee
authors.forename: Se-Young
authors.initials: SY
authors.lastname: Choung
authors.forename: Kwang-Won
authors.initials: KW
authors.lastname: Lee
mesh_terms: D000085:Acetates / Q000737:chemistry; D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D045744:Cell Line, Tumor; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D027443:Lindera / Q000737:chemistry*; D008297:Male; D008362:Mannosides / Q000302:isolation & purification* / Q000494:pharmacology; D008543:Melanins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D008546:Melanoma, Experimental / Q000378:metabolism; D051739:Microphthalmia-Associated Transcription Factor / Q000235:genetics / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D010851:Picrates / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000302:isolation & purification* / Q000494:pharmacology; D061251:Primary Cell Culture; D044945:Proanthocyanidins / Q000302:isolation & purification* / Q000494:pharmacology; D011794:Quercetin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
chemical_list: D000085:Acetates; D000975:Antioxidants; D001713:Biphenyl Compounds; D008362:Mannosides; D008543:Melanins; D051739:Microphthalmia-Associated Transcription Factor; C497236:Mitf protein, rat; D010851:Picrates; D010936:Plant Extracts; D044945:Proanthocyanidins; C012526:quercitrin; C477954:afzelin; C007650:ethyl acetate; D020122:tert-Butylhydroperoxide; D011794:Quercetin; C004931:1,1-diphenyl-2-picrylhydrazyl; D014442:Monophenol Monooxygenase; D048049:Extracellular Signal-Regulated MAP Kinases