title: [Using the stable HSPA1A promoter-driven luciferase reporter HepG2 cells to assess the overall toxicity of coke oven emissions].
abstract: OBJECTIVE
  Using the stable HSPA1A (HSP70-1) promoter-driven luciferase reporter HepG2 cells (HepG2/HSPA1A cells) to assess the overall toxicity of coke oven emissions.
  
  
  METHODS
  The stable HepG2/HSPA1A cells were treated with different concentrations of coke oven emissions (COEs) collected from the top, side, and bottom of a coke oven battery for 24 h. After the treatments, luciferase activity, cell viability, malondialdehyde (MDA) concentration, Olive tail moment, and micronuclei frequency were determined, respectively.
  
  
  RESULTS
  The bottom COEs induced significant increases (P < 0.01) in relative luciferase activity up to 1.4 times the control level at 0.15 µg/L. The low dose of side COEs (0.02 µg/L) led to a significant increase (P < 0.01) in relative luciferase activity that progressively increased to 2.1 times the control level at 65.4 µg/L. The top COEs produced a strong dose-dependent induction of relative luciferase activity up to over 5 times the control level at the highest concentration tested (202 µg/L). In HepG2/HSPA1A cells treated with the bottom COEs, relative luciferase activity was positively correlated with MDA concentration (r = 0.404, P < 0.05). For the three COEs samples, positive correlations were observed between relative luciferase activity and Olive tail moment and micronuclei frequency.
  
  
  CONCLUSIONS
  The relative luciferase activity in HepG2/HSPA1A cells can sensitively reflect the overall toxicity of COEs. The stable HepG2/HSPA1A cells can be used for rapid screening of the overall toxicity of complex air pollutants in the workplace.
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Li-li
authors.initials: LL
authors.lastname: Xin
authors.forename: Xiao-hai
authors.initials: XH
authors.lastname: Li
authors.forename: Hua-xin
authors.initials: HX
authors.lastname: Deng
authors.forename: Dan
authors.initials: D
authors.lastname: Kuang
authors.forename: Xia-yun
authors.initials: XY
authors.lastname: Dai
authors.forename: Su-Li
authors.initials: SL
authors.lastname: Huang
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Mei-an
authors.initials: MA
authors.lastname: He
authors.forename: R William
authors.initials: RW
authors.lastname: Currie
authors.forename: Tang-chun
authors.initials: TC
authors.lastname: Wu
mesh_terms: D003077:Coke / Q000633:toxicity*; D017930:Genes, Reporter; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D056945:Hep G2 Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008315:Malondialdehyde / Q000032:analysis; D048629:Micronuclei, Chromosome-Defective; D016273:Occupational Exposure; D011401:Promoter Regions, Genetic; D018675:Toxicity Tests
chemical_list: D003077:Coke; D018840:HSP70 Heat-Shock Proteins; C490786:HSPA1A protein, human; D008315:Malondialdehyde; D008156:Luciferases