title: Diterpenoids with anti-inflammatory activity from the wood of Cunninghamia konishii.
abstract: Two new diterpenoids, konishone (1) and 3b-hydroxy-5,6-dehydrosugiol (2), along with three known diterpenoids--hinokiol (3), sugiol (4), and 12-hydroxy-6,7-secoabieta-8,11,13-triene-6,7-dial (5)--were isolated from the wood of Cunninghamia konishii. Compound 1 is a novel skeleton of the 7,20-dinorabietane-type diterpene. In addition, when RAW264.7 macrophages were treated with different concentrations of compounds 1, 3, and 5 together with LPS, a significant concentration-dependent inhibition of NO production was detected. The IC₅₀ values for inhibition of nitrite production of compounds 1, 3, and 5 were about 9.8 ± 0.7, 7.9 ± 0.9, and 9.3 ± 1.3 μg/mL, respectively. This study presents the potential utilization of compounds 1, 3, and 5, as lead compounds for the development of anti-inflammatory drugs.
authors.affiliation: Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung 404, Taiwan.
authors.forename: Yu-Chang
authors.initials: YC
authors.lastname: Chen
authors.forename: Yen-Cheng
authors.initials: YC
authors.lastname: Li
authors.forename: Bang-Jau
authors.initials: BJ
authors.lastname: You
authors.forename: Wen-Te
authors.initials: WT
authors.lastname: Chang
authors.forename: Louis Kuoping
authors.initials: LK
authors.lastname: Chao
authors.forename: Lee-Chiang
authors.initials: LC
authors.lastname: Lo
authors.forename: Sheng-Yang
authors.initials: SY
authors.lastname: Wang
authors.forename: Guan-Jhong
authors.initials: GJ
authors.lastname: Huang
authors.forename: Yueh-Hsiung
authors.initials: YH
authors.lastname: Kuo
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D032486:Cunninghamia / Q000737:chemistry*; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D014934:Wood / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D004224:Diterpenes; D008070:Lipopolysaccharides; C581689:konishone; C519399:secoferruginol; C501333:sugiol; D009569:Nitric Oxide