title: The effects of copper on Na(+)/K (+)-ATPase and aquaporin expression in two euryhaline invertebrates.
abstract: We used immunocytochemical and fluorometric techniques to show that gill cells of two marine invertebrates, the crab Neohelice granulata (osmoregulator) and the clam Mesodesma mactroides (osmoconformer), increase the expression of membrane transporters [Na(+)/K(+)-ATPase and aquaporin (AQP1)] after whole-animals exposure (96 h) to sublethal concentrations of copper in water of salinity 30 ppt, when both clams and crabs are isosmotic with respect to the environmental medium. A plausible interpretation of our findings is that this increased expression in membrane transporters may serve as an attempt to ameliorate the deleterious effects of copper on the mechanisms involved in ion and volume regulation in gill cells.
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Rio Grande (FURG), Rio Grande, RS, Brazil.
authors.forename: R T
authors.initials: RT
authors.lastname: Boyle
authors.forename: L F
authors.initials: LF
authors.lastname: Oliveira
authors.forename: A
authors.initials: A
authors.lastname: Bianchini
authors.forename: M M
authors.initials: MM
authors.lastname: Souza
mesh_terms: D000818:Animals; D051398:Aquaporin 1 / Q000096:biosynthesis*; D049872:Bivalvia / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D003386:Brachyura / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D003300:Copper / Q000633:toxicity*; D004784:Environmental Monitoring; D005880:Gills / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D009995:Osmosis; D054712:Salinity; D000254:Sodium-Potassium-Exchanging ATPase / Q000096:biosynthesis*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D051398:Aquaporin 1; D003300:Copper; D000254:Sodium-Potassium-Exchanging ATPase