title: MicroRNA expression in chronic lymphocytic leukemia developing autoimmune hemolytic anemia.
abstract: Chronic lymphocytic leukemia (CLL) is frequently associated with autoimmune hemolytic anemia (AIHA). However, the mechanisms governing the association between CLL and AIHA are poorly understood. MicroRNAs (miRNAs) have been associated with different clinico-biological forms of CLL and are also known to play a substantial role in autoimmunity. However, there are no studies correlating miRNA expression with the likelihood that patients with CLL will develop AIHA. In this study, we found that malignant B-cells from patients with CLL subsequently developing AIHA present nine down-regulated (i.e. miR-19a, miR-20a, miR-29c, miR-146b-5p, miR-186, miR-223, miR-324-3p, miR-484 and miR-660) miRNAs. Interestingly, two of these miRNAs (i.e. miR-20a and miR-146b-5p) are involved in autoimmune phenomena, and one (i.e. miR-146b-5p) in both autoimmunity and CLL. Furthermore, we demonstrated that miR-146b-5p modulates CD80, a molecule associated with the B-T-cell synapse and in restoration of the antigen presenting cell capacity of CLL cells.
authors.affiliation: Institute of Hematology and Oncology, Hospital Clínic, IDIBAPS, University of Barcelona, Barcelona, Spain.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Ferrer
authors.forename: Alfons
authors.initials: A
authors.lastname: Navarro
authors.forename: Kate
authors.initials: K
authors.lastname: Hodgson
authors.forename: Marta
authors.initials: M
authors.lastname: Aymerich
authors.forename: Arturo
authors.initials: A
authors.lastname: Pereira
authors.forename: Tycho
authors.initials: T
authors.lastname: Baumann
authors.forename: Mariano
authors.initials: M
authors.lastname: Monzo
authors.forename: Carol
authors.initials: C
authors.lastname: Moreno
authors.forename: Emili
authors.initials: E
authors.lastname: Montserrat
mesh_terms: D000744:Anemia, Hemolytic, Autoimmune / Q000209:etiology*; D018122:B7-1 Antigen / Q000235:genetics / Q000378:metabolism; D016000:Cluster Analysis; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000150:complications* / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D015203:Reproducibility of Results
chemical_list: D018122:B7-1 Antigen; C542542:MIRN146 microRNA, human; D035683:MicroRNAs