title: Enrichment of methylated DNA by methyl-CpG immunoprecipitation.
abstract: Normal DNA methylation is an epigenetic modification required for proper development. Aberrant DNA methylation, in contrast, is frequently observed in many different malignancies including leukemias and lymphomas. Global DNA methylation profiling addresses the methylated sequences (methylome) of patient genomes to identify disease-specific methylation patterns. Workload in methylome analyses can be considerably reduced by methylome enrichment using proteins or antibodies with high affinity to methylated DNA. Methyl-CpG Immunoprecipitation (MCIp) employs an immobilized recombinant human methyl-CpG binding domain protein 2, MBD2, which binds methylated CpGs in double-stranded DNA. Elution with increasing salt concentrations allows the fractionated enrichment of different degrees of methylation.
authors.affiliation: Department of Epigenomics and Cancer Risk Factors, German Cancer Research Center (DKFZ), Heidelberg, Germany.
authors.forename: Miriam
authors.initials: M
authors.lastname: Sonnet
authors.forename: Constance
authors.initials: C
authors.lastname: Baer
authors.forename: Michael
authors.initials: M
authors.lastname: Rehli
authors.forename: Dieter
authors.initials: D
authors.lastname: Weichenhan
authors.forename: Christoph
authors.initials: C
authors.lastname: Plass
mesh_terms: D018899:CpG Islands; D004247:DNA / Q000235:genetics; D019175:DNA Methylation; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D023281:Genomics; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D047468:Immunoprecipitation / Q000379:methods*; D059346:Magnets / Q000737:chemistry; D008863:Microspheres; D013010:Sonication; D013205:Staphylococcal Protein A / Q000737:chemistry
chemical_list: D004268:DNA-Binding Proteins; D055767:Immobilized Proteins; C115368:MBD2 protein; D013205:Staphylococcal Protein A; D004247:DNA