title: In vitro and in vivo anticancer activity of aconitine on melanoma cell line B16.
abstract: The anti-tumor effect of aconitine in melanoma cell line B16 has been studied in this paper. We found that B16 cells showed significantly reduced growth rates and increased apoptotic effects in the presence of aconitine. Furthermore, aconitine inhibited the PI3K/AKT and MAPK/ERK1/2 signaling pathways, thus regulating the levels of protein and mRNA of PCNA and apoptotic related signaling molecules. Above all, we found that aconitine showed an anti-melanoma effect in suppressing tumor growth in vivo. In conclusion, we show that aconitine may be a useful anticancer drug in the future.
authors.affiliation: Dermatology Department, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.forename: Xiaonian
authors.initials: X
authors.lastname: Lu
authors.forename: Ziwen
authors.initials: Z
authors.lastname: Long
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zhu
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Yang
authors.forename: Jinhua
authors.initials: J
authors.lastname: Xu
mesh_terms: D000157:Aconitine / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006801:Humans; D020128:Inhibitory Concentration 50; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D012878:Skin Neoplasms / Q000188:drug therapy* / Q000473:pathology; D047368:Tumor Burden / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases; D000157:Aconitine