title: Functional characterization of motif sequences under purifying selection.
abstract: Diverse life forms are driven by the evolution of gene regulatory programs including changes in regulator proteins and cis-regulatory elements. Alterations of cis-regulatory elements are likely to dominate the evolution of the gene regulatory networks, as they are subjected to smaller selective constraints compared with proteins and hence may evolve quickly to adapt the environment. Prior studies on cis-regulatory element evolution focus primarily on sequence substitutions of known transcription factor-binding motifs. However, evolutionary models for the dynamics of motif occurrence are relatively rare, and comprehensive characterization of the evolution of all possible motif sequences has not been pursued. In the present study, we propose an algorithm to estimate the strength of purifying selection of a motif sequence based on an evolutionary model capturing the birth and death of motif occurrences on promoters. We term this measure as the 'evolutionary retention coefficient', as it is related yet distinct from the canonical definition of selection coefficient in population genetics. Using this algorithm, we estimate and report the evolutionary retention coefficients of all possible 10-nucleotide sequences from the aligned promoter sequences of 27 748. orthologous gene families in 34 mammalian species. Intriguingly, the evolutionary retention coefficients of motifs are intimately associated with their functional relevance. Top-ranking motifs (sorted by evolutionary retention coefficients) are significantly enriched with transcription factor-binding sequences according to the curated knowledge from the TRANSFAC database and the ChIP-seq data generated from the ENCODE Consortium. Moreover, genes harbouring high-scoring motifs on their promoters retain significantly coherent expression profiles, and those genes are over-represented in the functional classes involved in gene regulation. The validation results reveal the dependencies between natural selection and functions of cis-regulatory elements and shed light on the evolution of gene regulatory networks.
authors.affiliation: Institute of Statistical Science, Academia Sinica, Taipei, Taiwan, ROC.
authors.forename: De-Hua
authors.initials: DH
authors.lastname: Chen
authors.forename: Andrew Ying-Fei
authors.initials: AY
authors.lastname: Chang
authors.forename: Ben-Yang
authors.initials: BY
authors.lastname: Liao
authors.forename: Chen-Hsiang
authors.initials: CH
authors.lastname: Yeang
mesh_terms: D000465:Algorithms; D000818:Animals; D001665:Binding Sites; D019143:Evolution, Molecular; D005796:Genes; D006801:Humans; D051379:Mice; D059372:Nucleotide Motifs; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D014157:Transcription Factors