title: Chemical profile, radical scavenging and cytotoxic activity of yellow gentian leaves (Genitaneae luteaefolium) grown in northern regions of Montenegro.
abstract: LC-ESI-MS and HPLC were used for the identification of the constituents from G. lutea leaves collected at different localities, as well as for quantification of the main compounds. Seven secoiridoids, five C-glucoflavones and three xanthones, were identified. Swertiamarin derivatives, namely eustomorusside (2), eustomoside (3) and septemfidoside (5), were detected in G. lutea for the first time. Concentrations of five constituents (swertiamarin, gentiopicrin, isovitexin, mangiferin and isogentisin) were determined. The relationship between concentrations of y-pyrones and altitude was observed with statistically significant correlation (r = 0.94). The extracts were also evaluated for their content of total phenolics, and antiradical and cytotoxic activities. The total phenolics content ranged from 7.7 to 12.7 mg GAE/g, and the IC50 values for DPPH radical scavenging activity varied between 0.45 to 2.02 mg/mL. The leaf extract exhibited moderate cytotoxic effects toward HeLa cells with an IC50 value of 41.1 microg/mL, while gentiopicrin, mangiferin and isogentisin exerted strong activity against HeLa cells, with IC50 values ranging from 5.7 to 8.8 microg/mL. The results confirm the traditional usage of G. lutea leaves and also suggest their possible utilization as hepatoprotective, hypoglycemic and anti-inflammatory agents.
authors.affiliation: Biotechnical Faculty, Center for Continental Fruit-growing, Sandzadzka BB, 84000 Bijelo Polje, Montenegro.
authors.forename: Jasmina
authors.initials: J
authors.lastname: Balijagić
authors.forename: Teodora
authors.initials: T
authors.lastname: Janković
authors.forename: Gordana
authors.initials: G
authors.lastname: Zdunić
authors.forename: Jelena
authors.initials: J
authors.lastname: Bosković
authors.forename: Katarina
authors.initials: K
authors.lastname: Savikin
authors.forename: Dejan
authors.initials: D
authors.lastname: Godevac
authors.forename: Tatjana
authors.initials: T
authors.lastname: Stanojković
authors.forename: Miodrag
authors.initials: M
authors.lastname: Jovancević
authors.forename: Nebojsa
authors.initials: N
authors.lastname: Menković
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification*; D004354:Drug Screening Assays, Antitumor; D016166:Free Radical Scavengers / Q000302:isolation & purification*; D030012:Gentiana / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D039823:Iridoids / Q000302:isolation & purification*; D054548:Montenegro; D018515:Plant Leaves / Q000737:chemistry; D044004:Xanthones / Q000302:isolation & purification*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D016166:Free Radical Scavengers; D039823:Iridoids; D044004:Xanthones