title: Intramyocardial delivery of mesenchymal stem cell-seeded hydrogel preserves cardiac function and attenuates ventricular remodeling after myocardial infarction.
abstract: BACKGROUND
  To improve the efficacy of bone marrow-derived mesenchymal stem cell (MSC) therapy targeted to infarcted myocardium, we investigated whether a self-setting silanized hydroxypropyl methylcellulose (Si-HPMC) hydrogel seeded with MSC (MSC+hydrogel) could preserve cardiac function and attenuate left ventricular (LV) remodeling during an 8-week follow-up study in a rat model of myocardial infarction (MI).
  
  
  RESULTS
  Si-HPMC hydrogel alone, MSC alone or MSC+hydrogel were injected into the myocardium immediately after coronary artery ligation in female Lewis rats. Animals in the MSC+hydrogel group showed an increase in cardiac function up to 28 days after MI and a mid-term prevention of cardiac function alteration at day 56. Histological analyses indicated that the injection of MSC+hydrogel induced a decrease in MI size and an increase in scar thickness and ultimately limited the transmural extent of MI. These findings show that intramyocardial injection of MSC+hydrogel induced short-term recovery of ventricular function and mid-term attenuation of remodeling after MI.
  
  
  CONCLUSIONS
  These beneficial effects may be related to the specific scaffolding properties of the Si-HPMC hydrogel that may provide the ability to support MSC injection and engraftment within myocardium.
authors.affiliation: INSERM UMR1087, CNRS UMR6291, l'institut du thorax, Nantes, France.
authors.forename: Eva
authors.initials: E
authors.lastname: Mathieu
authors.forename: Guillaume
authors.initials: G
authors.lastname: Lamirault
authors.forename: Claire
authors.initials: C
authors.lastname: Toquet
authors.forename: Pierre
authors.initials: P
authors.lastname: Lhommet
authors.forename: Emilie
authors.initials: E
authors.lastname: Rederstorff
authors.forename: Sophie
authors.initials: S
authors.lastname: Sourice
authors.forename: Kevin
authors.initials: K
authors.lastname: Biteau
authors.forename: Philippe
authors.initials: P
authors.lastname: Hulin
authors.forename: Virginie
authors.initials: V
authors.lastname: Forest
authors.forename: Pierre
authors.initials: P
authors.lastname: Weiss
authors.forename: Jérôme
authors.initials: J
authors.lastname: Guicheux
authors.forename: Patricia
authors.initials: P
authors.lastname: Lemarchand
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002470:Cell Survival; D058948:Cell Tracking; D004195:Disease Models, Animal; D004562:Electrocardiography; D005260:Female; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells; D009203:Myocardial Infarction / Q000473:pathology / Q000503:physiopathology* / Q000628:therapy*; D009206:Myocardium / Q000473:pathology; D051381:Rats; D013997:Time Factors; D020257:Ventricular Remodeling
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate