title: Effect of 2,4-thiazolidinedione on limousin cattle growth and on muscle and adipose tissue metabolism.
abstract: The main adipogenic transcription factor PPARγ possesses high affinity to 2,4-TZD, a member of the Thiazolidinedione family of insulin-sensitizing compounds used as adipogenic agents. We evaluated 2,4-TZD's effect on bovine growth and PPAR tissue expression. Seventeen Limousin bulls (18 month-old; 350 kg body weight (BW)) were assigned into 2 treatments: control and 2,4-TZD (8 mg/70 kg BW) and were fed until bulls reached 500 kg BW. They were weighed and their blood was sampled. DNA, RNA, and protein were determined in liver; skeletal muscle; subcutaneous (SC), omental, perirenal adipose tissues (AT) to determine protein synthesis rate and cellular size. Expression of PPAR mRNA was measured in liver and muscle (PPARα, -δ, and -γ) and SC adipose tissue (γ) by real-time PCR. No significant differences were found (P > 0.1) in weight gain, days on feed, and carcass quality. Muscle synthesis was greater in controls (P < 0.05); cell size was larger with 2,4-TZD (P < 0.05). PPARα, -δ, and -γ expressions with 2,4-TZD in liver were lower (P < 0.01) than in muscle. No differences were found for PPARγ mRNA expression in SCAT. The results suggest the potential use of 2,4-TZD in beef cattle diets, because it improves AT differentiation, liver, and muscle fatty acid oxidation that, therefore, might improve energy efficiency.
authors.affiliation: Programa de Posgrado en Ciencias de la Producción y de la Salud Animal, Universidad Nacional Autónoma de México (UNAM), 54714 Mexico City, DF, Mexico.
authors.forename: M
authors.initials: M
authors.lastname: Arévalo-Turrubiarte
authors.forename: L
authors.initials: L
authors.lastname: González-Dávalos
authors.forename: A
authors.initials: A
authors.lastname: Yabuta
authors.forename: J D
authors.initials: JD
authors.lastname: Garza
authors.forename: J L
authors.initials: JL
authors.lastname: Dávalos
authors.forename: O
authors.initials: O
authors.lastname: Mora
authors.forename: A
authors.initials: A
authors.lastname: Shimada