title: Periplasmic expression of a Saccharophagus cellodextrinase enables E. coli to ferment cellodextrin.
abstract: Metabolic engineering has been successful in generating highly efficient Escherichia coli catalysts for production of biofuels and other useful products. However, most of these engineered biocatalysts are only effective when glucose is used as the starting substrate. Strategies to overcome this limitation in the past almost exclusively relied on extracellular secretion or surface display of a β-glucosidase. We show here, for the first time, a periplasmic expression of a Sacchrophagus degradans cellodextrinase (Ced3A) as a successful strategy to enable E. coli to use cellodextrin. The engineered strain was able to grow with cellodextrin as sole carbon source. Additionally, we show that penetration of cellodextrin into periplasmic space was enhanced by using a mutant with leaky outer membrane. Furthermore, we demonstrate that the catalyst can efficiently ferment cellodextrin to lactic acid with about 80 % yield. The ability of a biocatalyst to use cellodextrin should make it useful in consolidated bioprocessing of cellulose.
authors.affiliation: School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332-0100, USA.
authors.forename: Charles
authors.initials: C
authors.lastname: Rutter
authors.forename: Zichao
authors.initials: Z
authors.lastname: Mao
authors.forename: Rachel
authors.initials: R
authors.lastname: Chen
mesh_terms: D034241:Alteromonadaceae / Q000201:enzymology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002480:Cellulase / Q000235:genetics* / Q000378:metabolism; D002482:Cellulose / Q000031:analogs & derivatives* / Q000378:metabolism; D003912:Dextrins / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005285:Fermentation; D015870:Gene Expression; D060847:Metabolic Engineering; D019897:Periplasm / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D003912:Dextrins; D002482:Cellulose; C068994:cellodextrin; D002480:Cellulase