title: [The effect  and    mechanism  of SU11248 on proliferation of nasopharyngeal carcinoma cell line CNE-2].
abstract: OBJECTIVE
  To investigate the effect of sunitinib malate (SU11284) on proliferation of nasopharyngeal carcinoma cell line CNE-2 and explore its mechanism.
  
  
  METHODS
  Nasopharyngeal carcinoma cell line CNE-2 was treated with SU11248 in vitro, and the change in the proliferation of CNE-2 cell line was observed by MTT assay; Real-time fluorescence quantitative PCR (FQ-PCR) and Western blotting were used to detect the levels of P27(KIP1);, cyclin G1 mRNA and protein in CNE-2 cells after treated with 2.5 μg/mL SU11284 for different durations.
  
  
  RESULTS
  SU11248 inhibited the proliferation of CNE-2 cells in a concentration- and time-dependent manner. The 50% inhibiting concentration (IC50) was 2.5 μg/mL. Moreover, SU11248 treatment concentration-dependently decreased cyclin G1 mRNA and protein expressions (P<0.05), in contrast, it time-dependently increased P27(KIP1); mRNA and protein levels (P<0.05).
  
  
  CONCLUSIONS
  SU11248 may significantly inhibit the proliferation of CNE-2 cells through up-regulating P27(KIP1); and down-regulating cyclin G1.
authors.affiliation: First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, China. yiyitx@163.com
authors.forename: Qiaozhi
authors.initials: Q
authors.lastname: Jin
authors.forename: Xingxing
authors.initials: X
authors.lastname: Ye
authors.forename: Wubing
authors.initials: W
authors.lastname: Chen
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Cai
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D002277:Carcinoma; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D056766:Cyclin G1 / Q000235:genetics / Q000378:metabolism; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism*; D011758:Pyrroles / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000077210:Sunitinib
chemical_list: D000970:Antineoplastic Agents; D056766:Cyclin G1; D007211:Indoles; D011758:Pyrroles; D012333:RNA, Messenger; D050760:Cyclin-Dependent Kinase Inhibitor p27; D000077210:Sunitinib