title: Sensing of glycoprotein via a biomimetic sensor based on molecularly imprinted polymers and graphene-Au nanoparticles.
abstract: A novel strategy was proposed for preparing a highly sensitive glycoprotein sensor based on molecularly imprinted polymers (MIP), which was electropolymerized with o-phenylenediamine and 3-aminophenylboronic acid monohydrate in the presence of template molecules (bovine serum albumin (BSA)). Sensitivity improved dramatically owing to the application of a graphene-Au nanoparticles hybrid as the electrode modifier, and the immobilization of a large amount of 6-ferrocenylhexanethiol, as the electroactive species, onto nanoparticles. The quantification of BSA was realized by detecting the electrochemical oxidation signal of 6-ferrocenylhexanethiol, which was bonded onto the electrode. Under optimized conditions, a good relationship was obtained between the response current and logarithm of BSA concentration in the range of 1.0 × 10(-11) to 1.0 × 10(-5) g mL(-1) with a detection limit of 7.5 × 10(-12) g mL(-1) (S/N = 3). The resulting MIP sensor displayed good selectivity, reproducibility and stability.
authors.affiliation: College of Chemistry and Material Science, Shandong Agricultural University, Taian, Shandong 271018, China.
authors.forename: Xindong
authors.initials: X
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Dong
authors.forename: Huami
authors.initials: H
authors.lastname: Ming
authors.forename: Shiyun
authors.initials: S
authors.lastname: Ai
mesh_terms: D000818:Animals; D032701:Biomimetics / Q000379:methods; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D006023:Glycoproteins / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000032:analysis
chemical_list: D006023:Glycoproteins; D011108:Polymers; D012710:Serum Albumin, Bovine; D006046:Gold; D006108:Graphite