title: Cytological, immunocytochemical, ultrastructural and growth characterization of the rainbow trout liver cell line RTL-W1.
abstract: Despite its wide use in toxicology, a detailed characterization of RTL-W1 cell line lagged behind leaving ambiguities about its cell origin. We aimed to better characterize the line regarding cell phenotype and tumorigenic state. We studied RTL-W1 cells in monolayers and in (4-22-week-old) aggregates considering: (a) morphology (light and electron microscopy); (b) immunophenotype using AE1/AE3, vimentin, Cam5.2, CK7 and CK19 and e-cadherin antibodies and (c) growth behavior. RTL-W1 organelle content is constituted basically by mitochondria and abundant free ribosomes, with no (cytochemically) detectable peroxisomes and lysosomes. Immunocytochemistry showed a strong marking for AE1/AE3 and vimentin (in a cell subset). Since AE1/AE3 stained biliary epithelial ducts in trout liver, and considering the morphological characteristics and long term culture, RTL-W1 cells seem more similar to bile preductular epithelial cells (considered as stem cells in teleost liver). Also, we observed abnormal nuclear features described for both malignant cell lines and stem cells, so we could not conclude about tumorigenicity. Cell aggregates had signs of hepatocytic differentiation, such as the development of RER and microvillus-like projections into intercellular spaces. The morphological resemblance to the original tissue suggests that aggregates could have an added value in metabolic as well as in cell-to-cell interaction studies.
authors.affiliation: Laboratory of Histology and Embryology, Institute of Biomedical Sciences Abel Salazar ICBAS, University of Porto U. Porto, Rua de Jorge Viterbo Ferreira no. 228, 4050-313 Porto, Portugal.
authors.forename: F
authors.initials: F
authors.lastname: Malhão
authors.forename: R
authors.initials: R
authors.lastname: Urbatzka
authors.forename: J M
authors.initials: JM
authors.lastname: Navas
authors.forename: C
authors.initials: C
authors.lastname: Cruzeiro
authors.forename: R A F
authors.initials: RA
authors.lastname: Monteiro
authors.forename: E
authors.initials: E
authors.lastname: Rocha
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism / Q000648:ultrastructure; D002449:Cell Aggregation; D002450:Cell Communication; D002460:Cell Line / Q000166:cytology* / Q000276:immunology / Q000648:ultrastructure; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism / Q000648:ultrastructure; D008099:Liver / Q000166:cytology* / Q000254:growth & development* / Q000276:immunology / Q000648:ultrastructure; D017686:Oncorhynchus mykiss / Q000254:growth & development / Q000276:immunology
chemical_list: D015820:Cadherins; D019363:Cytochrome P-450 CYP1A1