title: Mapping B-cell linear epitopes of NS3 protein of bovine viral diarrhea virus.
abstract: Bovine viral diarrhea virus (BVDV) is a member of the genus Pestivirus within the family Flaviviridae. NS3 is one of the immunodominance regions of the BVDV viral proteins. To identify the potential B-cell linear antigenic epitopes within BVDV NS3 region, serial overlapping truncations covering the whole region were expressed and purified, and screened by multistep of Western-blot. We found ((1)VCKKITEHERCHVNI(15)), ((20)AFFGVMPRGTTPRAPVR(36)), ((46)RRGLETGWAYTHQGGI(61)), ((281)EGDMATGITYASYGYFC(297)), ((426)YSGEDPANLRVVTSQSPYVVVATNAIESGV(455)) and ((481)FIVTGLKRMAVTVGEQA(497)) can be recognized by the BVDV infected bovine serum. These proteins have been confirmed by indirect enzyme-linked immunosorbent assay (I-ELISA). The results of this study might open new perspectives on the structure and antibody-antigen reaction of the non-structural proteins and may aid in the clinical application as well.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, 150030, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Jia
authors.forename: Kai
authors.initials: K
authors.lastname: Wen
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.forename: Mingchun
authors.initials: M
authors.lastname: Gao
authors.forename: Bo
authors.initials: B
authors.lastname: Ma
authors.forename: Wenlong
authors.initials: W
authors.lastname: Zhang
authors.forename: Junwei
authors.initials: J
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000276:immunology / Q000821:virology; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics* / Q000276:immunology*; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000235:genetics; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D010447:Peptide Hydrolases / Q000235:genetics* / Q000276:immunology*; D020365:RNA Helicases / Q000235:genetics* / Q000276:immunology*; D017386:Sequence Homology, Amino Acid; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000276:immunology*; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D018985:Epitopes, B-Lymphocyte; D010446:Peptide Fragments; D010447:Peptide Hydrolases; D020365:RNA Helicases; D017361:Viral Nonstructural Proteins; C086730:p80 protein, bovine viral diarrhea virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases