title: Rapid and serial quantification of adhesion forces of yeast and Mammalian cells.
abstract: Cell adhesion to surfaces represents the basis for niche colonization and survival. Here we establish serial quantification of adhesion forces of different cell types using a single probe. The pace of single-cell force-spectroscopy was accelerated to up to 200 yeast and 20 mammalian cells per probe when replacing the conventional cell trapping cantilever chemistry of atomic force microscopy by underpressure immobilization with fluidic force microscopy (FluidFM). In consequence, statistically relevant data could be recorded in a rapid manner, the spectrum of examinable cells was enlarged, and the cell physiology preserved until approached for force spectroscopy. Adhesion forces of Candida albicans increased from below 4 up to 16 nN at 37°C on hydrophobic surfaces, whereas a Δhgc1-mutant showed forces consistently below 4 nN. Monitoring adhesion of mammalian cells revealed mean adhesion forces of 600 nN of HeLa cells on fibronectin and were one order of magnitude higher than those observed for HEK cells.
authors.affiliation: Institute of Microbiology, ETH Zurich, Zurich, Switzerland.
authors.forename: Eva
authors.initials: E
authors.lastname: Potthoff
authors.forename: Orane
authors.initials: O
authors.lastname: Guillaume-Gentil
authors.forename: Dario
authors.initials: D
authors.lastname: Ossola
authors.forename: Jérôme
authors.initials: J
authors.lastname: Polesel-Maris
authors.forename: Salomé
authors.initials: S
authors.lastname: LeibundGut-Landmann
authors.forename: Tomaso
authors.initials: T
authors.lastname: Zambelli
authors.forename: Julia A
authors.initials: JA
authors.lastname: Vorholt
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D018625:Microscopy, Atomic Force; D013499:Surface Properties; D013696:Temperature; D015003:Yeasts / Q000502:physiology*