title: Preparation and characterization of vinculin-targeted polymer-lipid nanoparticle as intracellular delivery vehicle.
abstract: Intracellular delivery vehicles have been extensively investigated as these can serve as an effective tool in studying the cellular mechanism, by delivering functional protein to specific locations of the cells. In the current study, a polymer-lipid nanoparticle (PLN) system was developed as an intracellular delivery vehicle specifically targeting vinculin, a focal adhesion protein associated with cellular adhesive structures, such as focal adhesions and adherens junctions. The PLNs possessed an average size of 106 nm and had a positively charged surface. With a lower encapsulation efficiency 32% compared with poly(lactic-co-glycolic) acid (PLGA) nanoparticles (46%), the PLNs showed the sustained release profile of model drug BSA, while PLGA nanoparticles demonstrated an initial burst-release property. Cell-uptake experiments using mouse embryonic fibroblasts cultured in fibrin-fibronectin gels observed, under confocal microscope, that the anti-vinculin conjugated PLNs could successfully ship the cargo to the cytoplasm of fibroblasts, adhered to fibronectin-fibrin. With the use of cationic lipid, the unconjugated PLNs were shown to have high gene transfection efficiency. Furthermore, the unconjugated PLNs had nuclear-targeting capability in the absence of nuclear-localization signals. Therefore, the PLNs could be manipulated easily via different type of targeting ligands and could potentially be used as a powerful tool for cellular mechanism study, by delivering drugs to specific cellular organelles.
authors.affiliation: Department of Chemistry, Chemical Biology, and Biomedical Engineering, Stevens Institute of Technology, Hoboken, NJ 07030, USA.
authors.forename: Junping
authors.initials: J
authors.lastname: Wang
authors.forename: Ceren
authors.initials: C
authors.lastname: Ornek-Ballanco
authors.forename: Jiahua
authors.initials: J
authors.lastname: Xu
authors.forename: Weiguo
authors.initials: W
authors.lastname: Yang
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Yu
mesh_terms: D000818:Animals; D002460:Cell Line; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D042541:Intracellular Space / Q000737:chemistry / Q000378:metabolism*; D019344:Lactic Acid / Q000008:administration & dosage / Q000737:chemistry*; D051379:Mice; D008856:Microscopy, Fluorescence; D058990:Molecular Targeted Therapy; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D010316:Particle Size; D011100:Polyglycolic Acid / Q000008:administration & dosage / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000493:pharmacokinetics; D016596:Vinculin / Q000378:metabolism*; D014966:Xanthenes / Q000737:chemistry / Q000493:pharmacokinetics
chemical_list: D004337:Drug Carriers; D014966:Xanthenes; D016596:Vinculin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; C034657:Texas red
keywords: 3D gel; PLGA; targeting