title: Analysis of the lung transcriptome in Mycobacterium tuberculosis-infected mice reveals major differences in immune response pathways between TB-susceptible and resistant hosts.
abstract: Using whole genome microarrays, we compared changes in gene expression patterns in the lungs of TB-resistant A/Sn and TB-susceptible I/St mice at day 14 following infection with Mycobacterium tuberculosis H37Rv. Analyses of differentially expressed genes for representation of gene ontology terms and activation of regulatory pathways revealed interstrain differences in antigen presentation, NK, T and B cell activation pathways. In general, resistant A/Sn mice exhibited a more complex pattern and stronger activation of host defense pathways compared to the TB-susceptible I/St mouse strain. In addition, in I/St mice elevated activation of genes involved in neutrophil response was observed and confirmed by quantitative RT-PCR and histopathology. Furthermore, a specific post infection upregulation of cysteine protease inhibitors was found in susceptible I/St mice.
authors.affiliation: Laboratory for Immunogenetics, Central Institute for Tuberculosis, Moscow, Russia. shepelkovag@yahoo.com
authors.forename: Galina
authors.initials: G
authors.lastname: Shepelkova
authors.forename: Claudia
authors.initials: C
authors.lastname: Pommerenke
authors.forename: Rudi
authors.initials: R
authors.lastname: Alberts
authors.forename: Robert
authors.initials: R
authors.lastname: Geffers
authors.forename: Vladimir
authors.initials: V
authors.lastname: Evstifeev
authors.forename: Alexander
authors.initials: A
authors.lastname: Apt
authors.forename: Klaus
authors.initials: K
authors.lastname: Schughart
authors.forename: Esther
authors.initials: E
authors.lastname: Wilk
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000235:genetics; D004195:Disease Models, Animal; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000276:immunology; D020022:Genetic Predisposition to Disease; D006098:Granulocytes / Q000276:immunology; D008168:Lung / Q000276:immunology*; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008815:Mice, Inbred Strains; D020556:Neutrophil Infiltration / Q000235:genetics; D055333:Salivary Cystatins / Q000096:biosynthesis / Q000235:genetics; D013045:Species Specificity; D059467:Transcriptome / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000235:genetics* / Q000276:immunology*; D015854:Up-Regulation / Q000276:immunology
chemical_list: D055333:Salivary Cystatins