title: Contrast enhancement in light microscopy.
abstract: The optical microscope is a fundamental component of an image cytometry system. This unit covers the basic concepts of light microscopy, including Köhler illumination, resolution, contrast, and numerical aperture, and reviews the many types of instruments and techniques for contrast enhancement.
authors.affiliation: Carl Zeiss, Inc, Thornwood, New York.
authors.forename: H
authors.initials: H
authors.lastname: Ernst Keller
authors.affiliation: Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, Pennsylvania.
authors.forename: Simon
authors.initials: S
authors.lastname: Watkins
mesh_terms: D015350:Contrast Sensitivity; D006801:Humans; D019044:Image Cytometry / Q000295:instrumentation* / Q000379:methods; D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods; D008027:Light; D008029:Lighting; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods*; D008859:Microscopy, Polarization / Q000295:instrumentation / Q000379:methods; D008954:Models, Biological