title: β-galactosyl Yariv reagent binds to the β-1,3-galactan of arabinogalactan proteins.
abstract: Yariv phenylglycosides [1,3,5-tri(p-glycosyloxyphenylazo)-2,4,6-trihydroxybenzene] are a group of chemical compounds that selectively bind to arabinogalactan proteins (AGPs), a type of plant proteoglycan. Yariv phenylglycosides are widely used as cytochemical reagents to perturb the molecular functions of AGPs as well as for the detection, quantification, purification, and staining of AGPs. However, the target structure in AGPs to which Yariv phenylglycosides bind has not been determined. Here, we identify the structural element of AGPs required for the interaction with Yariv phenylglycosides by stepwise trimming of the arabinogalactan moieties using combinations of specific glycoside hydrolases. Whereas the precipitation with Yariv phenylglycosides (Yariv reactivity) of radish (Raphanus sativus) root AGP was not reduced after enzyme treatment to remove α-l-arabinofuranosyl and β-glucuronosyl residues and β-1,6-galactan side chains, it was completely lost after degradation of the β-1,3-galactan main chains. In addition, Yariv reactivity of gum arabic, a commercial product of acacia (Acacia senegal) AGPs, increased rather than decreased during the repeated degradation of β-1,6-galactan side chains by Smith degradation. Among various oligosaccharides corresponding to partial structures of AGPs, β-1,3-galactooligosaccharides longer than β-1,3-galactoheptaose exhibited significant precipitation with Yariv in a radial diffusion assay on agar. A pull-down assay using oligosaccharides cross linked to hydrazine beads detected an interaction of β-1,3-galactooligosaccharides longer than β-1,3-galactopentaose with Yariv phenylglycoside. To the contrary, no interaction with Yariv was detected for β-1,6-galactooligosaccharides of any length. Therefore, we conclude that Yariv phenylglycosides should be considered specific binding reagents for β-1,3-galactan chains longer than five residues, and seven residues are sufficient for cross linking, leading to precipitation of the Yariv phenylglycosides.
authors.affiliation: Division of Life Science, Graduate School of Science and Engineering , Saitama University, Sakura-ku, Saitama 338-8570, Japan.
authors.forename: Kiminari
authors.initials: K
authors.lastname: Kitazawa
authors.forename: Theodora
authors.initials: T
authors.lastname: Tryfona
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Yoshimi
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Susumu
authors.initials: S
authors.lastname: Kawauchi
authors.forename: Liudmil
authors.initials: L
authors.lastname: Antonov
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tanaka
authors.forename: Takashi
authors.initials: T
authors.lastname: Takahashi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kaneko
authors.forename: Paul
authors.initials: P
authors.lastname: Dupree
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Tsumuraya
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Kotake
mesh_terms: D050260:Carbohydrate Metabolism; D011232:Chemical Precipitation; D005685:Galactans / Q000737:chemistry / Q000378:metabolism*; D005960:Glucosides / Q000378:metabolism*; D008958:Models, Molecular; D009088:Mucoproteins / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism; D010696:Phloroglucinol / Q000031:analogs & derivatives* / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D031224:Raphanus / Q000378:metabolism
chemical_list: D005685:Galactans; D005960:Glucosides; D009088:Mucoproteins; D009844:Oligosaccharides; D010940:Plant Proteins; C104977:Yariv reagent; C006619:arabinogalactan proteins; C044347:1,6-galactan; D010696:Phloroglucinol; C005653:arabinogalactan