title: Transcriptome response to nitrosative stress in Rhodobacter sphaeroides 2.4.1.
abstract: The facultative photosynthetic bacterium Rhodobacter sphaeroides 2.4.1 has a nitric oxide-response transcriptional regulator, NnrR, and nitric oxide reductase (NOR), although it is incapable of denitrification. To investigate at the genomic level the physiological response to nitrosative stress of R. sphaeroides, the transcriptome profiles of strain 2.4.1 and its NnrR mutant were analyzed before and after exposure to nitrosating agents, S-nitrosoglutathione (GSNO) and sodium nitroprusside (SNP), under microaerobic conditions. GSNO and SNP affected the expression of different but overlapping sets of genes. Only a limited number of these genes, including the genes for NOR, were under the control of NnrR, and those genes were significantly upregulated by GSNO and by SNP. The oxygen-responsive regulator FnrL and a predicted iron-sensing regulator were perhaps also involved in the transcriptome response to reactive nitrogen species. Some genes, including hemN for heme biosynthesis, were subject to dual regulation by NnrR and FnrL.
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Texas, Houston, USA. aharai@mail.ecc.u-tokyo.ac.jp
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Arai
authors.forename: Jung Hyeob
authors.initials: JH
authors.lastname: Roh
authors.forename: Jesus M
authors.initials: JM
authors.lastname: Eraso
authors.forename: Samuel
authors.initials: S
authors.lastname: Kaplan
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003304:Coproporphyrinogen Oxidase / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D009154:Mutation; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D012242:Rhodobacter sphaeroides / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D026422:S-Nitrosoglutathione / Q000494:pharmacology; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome
chemical_list: D001426:Bacterial Proteins; D003304:Coproporphyrinogen Oxidase; D009569:Nitric Oxide; D020030:Nitric Oxide Donors; D009599:Nitroprusside; D010088:Oxidoreductases; D026422:S-Nitrosoglutathione; D015534:Trans-Activators; C096744:FnrL protein, Rhodobacter sphaeroides; C100897:NnrR protein, Rhodobacter sphaeroides; C109194:HemN protein, Bacteria; C034747:nitric-oxide reductase