title: Mucilaginibacter herbaticus sp. nov., isolated from the rhizosphere of the medicinal plant Angelica sinensis.
abstract: A strictly aerobic, Gram-staining-negative, non-motile and rod-shaped bacterial strain, DR-9(T), was isolated from rhizosphere soil of the medicinal herb Angelica sinensis. Strain DR-9(T) grew at 20-40 °C, at pH 4.0-9.0 and in the presence of 0-1 % (w/v) NaCl. The major fatty acids were iso-C15 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c and/or iso-C15 : 0 2-OH), MK-7 was the major isoprenoid quinone, and phosphatidylethanolamine and an unidentified aminophospholipid were the major polar lipids. A phylogenetic tree based on 16S rRNA gene sequences showed that strain DR-9(T) formed a lineage within the genus Mucilaginibacter and was closely related to Mucilaginibacter polysacchareus DRP28(T) (96.1 % sequence similarity), Mucilaginibacter myungsuensis HMD1056(T) (95.9 % sequence similarity), Mucilaginibacter ximonensis XM-003(T) (95.8 %) and Mucilaginibacter boryungensis BDR-9(T) (95.1 %). The status of strain DR-9(T) as a representative of a separate species was confirmed by DNA hybridization, with 38.6, 36.3 and 29.9 % DNA-DNA relatedness with M. polysacchareus DRP28(T), M. ximonensis XM-003(T) and M. boryungensis BDR-9(T), respectively. The genomic DNA G+C content of strain DR-9(T) was 49.8 %. These data suggest that strain DR-9(T) should be considered as a representative of a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter herbaticus sp. nov. is proposed. The type strain is DR-9(T) ( = KACC 16469(T) = NBRC 108839(T)).
authors.affiliation: Department of Microbial & Nano Materials, Mokwon University, 800, Doan-dong, Seo-gu, Daejeon, Republic of Korea.
authors.forename: Hae-Ran
authors.initials: HR
authors.lastname: Lee
authors.affiliation: Department of Microbial & Nano Materials, Mokwon University, 800, Doan-dong, Seo-gu, Daejeon, Republic of Korea.
authors.forename: Song-Ih
authors.initials: SI
authors.lastname: Han
authors.affiliation: College of Industrial Sciences, Kongju National University, Yesan 340-702, Republic of Korea.
authors.forename: Ki-Hyeong
authors.initials: KH
authors.lastname: Rhee
authors.affiliation: Institute of Microbial Ecology and Resources, Mokwon University, 800, Doan-dong, Seo-gu, Daejeon, Republic of Korea.|Department of Microbial & Nano Materials, Mokwon University, 800, Doan-dong, Seo-gu, Daejeon, Republic of Korea.
authors.forename: Kyung-Sook
authors.initials: KS
authors.lastname: Whang
mesh_terms: D029971:Angelica sinensis / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D010946:Plants, Medicinal / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013107:Sphingolipids / Q000032:analysis; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D013107:Sphingolipids; D024482:Vitamin K 2; C062629:menaquinone 7