title: Galactoglucomannan oligosaccharides are assumed to affect tracheary element formation via interaction with auxin in Zinnia xylogenic cell culture.
abstract: Galactoglucomannan oligosaccharides seem to interact with auxin in xylogenic cell culture, thus influencing mainly metaxylem-like tracheary element differentiation depending on timing with hormones and the process kinetics. Complex mapping of Zinnia mesophyll cell transdifferentiation into tracheary elements with or without prior cell division was documented after palisade and spongy parenchyma cell immobilization during the first 4 days of culture. Here, we report a positive effect of galactoglucomannan oligosaccharides on cell viability and density and higher metaxylem-like tracheary element formation in xylogenic cell culture. The maximal positive effect was achieved by the simultaneous addition of the oligosaccharides and growth hormones (auxin, cytokinin) to the cell culture medium. Moreover, a large number of metaxylem-like tracheary elements were observed in a low-auxin medium supplemented with oligosaccharides, but not in a low-cytokinin medium, suggesting a close relationship between auxin and the oligosaccharides during tracheary element formation.
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Dúbravská Cesta 9, 845 38, Bratislava, Slovakia, benovaa@yahoo.com.
authors.forename: Anna
authors.initials: A
authors.lastname: Kákošová
authors.forename: Catherine
authors.initials: C
authors.lastname: Digonnet
authors.forename: Deborah
authors.initials: D
authors.lastname: Goffner
authors.forename: Desana
authors.initials: D
authors.lastname: Lišková
mesh_terms: D019659:Asteraceae / Q000187:drug effects / Q000254:growth & development*; D018929:Cell Culture Techniques; D054338:Cell Transdifferentiation; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D003583:Cytokinins / Q000494:pharmacology; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008351:Mannans / Q000494:pharmacology*; D058503:Mesophyll Cells / Q000187:drug effects / Q000502:physiology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D052584:Xylem / Q000254:growth & development*
chemical_list: D003470:Culture Media; D003583:Cytokinins; D007210:Indoleacetic Acids; D008351:Mannans; D010937:Plant Growth Regulators; C443804:galactoglucomannan