title: The effect of plant tissue and vaccine formulation on the oral immunogenicity of a model plant-made antigen in sheep.
abstract: Antigen-specific antibody responses against a model antigen (the B subunit of the heat labile toxin of enterotoxigenic Escherichia coli, LTB) were studied in sheep following oral immunisation with plant-made and delivered vaccines. Delivery from a root-based vehicle resulted in antigen-specific immune responses in mucosal secretions of the abomasum and small intestine and mesenteric lymph nodes. Immune responses from the corresponding leaf-based vaccine were more robust and included stimulation of antigen-specific antibodies in mucosal secretions of the abomasum. These findings suggest that oral delivery of a plant bioencapsulated antigen can survive passage through the rumen to elicit mucosal and systemic immune responses in sheep. Moreover, the plant tissue used as the vaccine delivery vehicle affects the magnitude of these responses.
authors.affiliation: School of Biological Sciences, Monash University, Clayton, Victoria, Australia.
authors.forename: Assunta
authors.initials: A
authors.lastname: Pelosi
authors.forename: David
authors.initials: D
authors.lastname: Piedrafita
authors.forename: Giorgio
authors.initials: G
authors.lastname: De Guzman
authors.forename: Robert
authors.initials: R
authors.lastname: Shepherd
authors.forename: John D
authors.initials: JD
authors.lastname: Hamill
authors.forename: Els
authors.initials: E
authors.lastname: Meeusen
authors.forename: Amanda M
authors.initials: AM
authors.lastname: Walmsley
mesh_terms: D000284:Administration, Oral; D000818:Animals; D052179:Antigens, Plant / Q000276:immunology*; D001428:Bacterial Vaccines / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology*; D054307:Enterotoxigenic Escherichia coli / Q000276:immunology; D004768:Enterotoxins / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D004927:Escherichia coli Infections / Q000517:prevention & control; D007111:Immunity, Cellular / Q000187:drug effects; D007114:Immunization / Q000379:methods / Q000662:veterinary; D008297:Male; D008962:Models, Theoretical; D009061:Mouth Mucosa / Q000187:drug effects / Q000276:immunology; D030821:Plants, Genetically Modified / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012756:Sheep; D012757:Sheep Diseases / Q000517:prevention & control
chemical_list: D052179:Antigens, Plant; D001428:Bacterial Vaccines; D004768:Enterotoxins; D011994:Recombinant Proteins