title: An application of polymer-enhanced capillary transient isotachophoresis with an emissive boronic acid functionalized squarylium dye as an on-capillary labeling agent for gram-positive bacteria.
abstract: In this paper, the characterization and application of the "PectI" (polymer-enhanced capillary transient isotachophoresis) technique for the separation and detection of same genus, gram-positive bacteria, Bacillus globigii (Bg) and Bacillus subtilis, is demonstrated by employing a boronic acid-functionalized squarylium dye (SQ-BA) as an on-capillary labeling agent, including the quantitative performance and applicability to crude samples.  The effect of borate in the separation buffer was also investigated, which revealed that borate strongly affects the separation behavior of bacteria.
authors.affiliation: Graduate School of Science and Engineering, Saitama University, Sakura, Saitama, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Saito
authors.forename: Takeshi
authors.initials: T
authors.lastname: Maeda
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakazumi
authors.forename: Christa L
authors.initials: CL
authors.lastname: Colyer
mesh_terms: D001412:Bacillus subtilis / Q000302:isolation & purification; D001897:Boronic Acids / Q000737:chemistry*; D002138:Calibration; D003503:Cyclobutanes / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006094:Gram-Positive Bacteria / Q000302:isolation & purification*; D057625:Isotachophoresis / Q000379:methods*; D057230:Limit of Detection; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry; D013171:Spores, Bacterial / Q000302:isolation & purification
chemical_list: D001897:Boronic Acids; D003503:Cyclobutanes; D005456:Fluorescent Dyes; D011108:Polymers; D012822:Silicon Dioxide; C030042:squaric acid