title: Transcriptional program of ciliated epithelial cells reveals new cilium and centrosome components and links to human disease.
abstract: Defects in the centrosome and cilium are associated with a set of human diseases having diverse phenotypes. To further characterize the components that define the function of these organelles we determined the transcriptional profile of multiciliated tracheal epithelial cells. Cultures of mouse tracheal epithelial cells undergoing differentiation in vitro were derived from mice expressing GFP from the ciliated-cell specific FOXJ1 promoter (FOXJ1:GFP). The transcriptional profile of ciliating GFP+ cells from these cultures was defined at an early and a late time point during differentiation and was refined by subtraction of the profile of the non-ciliated GFP- cells. We identified 649 genes upregulated early, when most cells were forming basal bodies, and 73 genes genes upregulated late, when most cells were fully ciliated. Most, but not all, of known centrosome proteins are transcriptionally upregulated early, particularly Plk4, a master regulator of centriole formation. We found that three genes associated with human disease states, Mdm1, Mlf1, and Dyx1c1, are upregulated during ciliogenesis and localize to centrioles and cilia. This transcriptome for mammalian multiciliated epithelial cells identifies new candidate centrosome and cilia proteins, highlights similarities between components of motile and primary cilia, and identifies new links between cilia proteins and human disease.
authors.affiliation: Department of Biology, Stanford University, Stanford, CA, USA.
authors.forename: Ramona A
authors.initials: RA
authors.lastname: Hoh
authors.forename: Timothy R
authors.initials: TR
authors.lastname: Stowe
authors.forename: Erin
authors.initials: E
authors.lastname: Turk
authors.forename: Tim
authors.initials: T
authors.lastname: Stearns
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D018385:Centrosome / Q000378:metabolism*; D002923:Cilia / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D051858:Forkhead Transcription Factors / Q000502:physiology; D020869:Gene Expression Profiling; D030342:Genetic Diseases, Inborn; D006801:Humans; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D041681:NIH 3T3 Cells; D020411:Oligonucleotide Array Sequence Analysis; D014132:Trachea / Q000166:cytology / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D015415:Biomarkers; C481963:FOXJ1 protein, mouse; D051858:Forkhead Transcription Factors