title: Small-angle X-ray scattering study of self-assembling lipophilic guanines in organic solvents: G-quadruplex formation and cation effects in cyclohexane.
abstract: Lipophilic guanilic derivatives (lipoGs) dissolved in organic solvents can undergo different self-assembly pathways based on different H-bonded motifs, e.g., the cyclic discrete G-quartet, which forms in the presence of alkali-metal ions, and the "infinite" tape-like G-ribbon observed in the absence of ions. Using in-solution small-angle X-ray scattering, we analyzed a series of lipoGs dissolved in cyclohexane in the presence of different salts. The formation of G-quartet based supramolecular aggregates has been confirmed, evidencing the coexistence equilibrium of octamers and noncovalent molecular nanowires (the so-called G-quadruplexes). By global fitting the scattering data, the concentration of the two kinds of particles as well as the nanowire length have been derived as a function of temperature for the different compounds and salts. The thermodynamic parameters show that the self-assembly aggregation process is enthalpy driven, while the observed enthalpy-entropy compensation suggests that similar stacking interactions control the self-assembly of the different compounds. However, the strength of the stacking interactions, and then the nanowire stability, depends on the nature of templating cations and on their capacity to fill the central cavity of quadruplexes, with the order Sr(+) < Na(+) ≲ K(+).
authors.affiliation: Dipartimento di Scienze della Vita e dell'Ambiente, Biophysics Research Group, Università Politecnica delle Marche, via Brecce Bianche, I-60131 Ancona, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Gonnelli
authors.forename: M G
authors.initials: MG
authors.lastname: Ortore
authors.forename: E J
authors.initials: EJ
authors.lastname: Baldassarri
authors.forename: G P
authors.initials: GP
authors.lastname: Spada
authors.forename: S
authors.initials: S
authors.lastname: Pieraccini
authors.forename: R C
authors.initials: RC
authors.lastname: Perone
authors.forename: S S
authors.initials: SS
authors.lastname: Funari
authors.forename: P
authors.initials: P
authors.lastname: Mariani
mesh_terms: D002412:Cations / Q000737:chemistry; D003510:Cyclohexanes / Q000737:chemistry*; D054856:G-Quadruplexes; D006147:Guanine / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D012492:Salts / Q000737:chemistry; D053838:Scattering, Small Angle; D012997:Solvents / Q000737:chemistry; D013816:Thermodynamics; D014961:X-Ray Diffraction
chemical_list: D002412:Cations; D003510:Cyclohexanes; D012492:Salts; D012997:Solvents; C506365:Cyclohexane; D006147:Guanine