title: [Preliminary study on aberrant expression of miRNAs related to the formation of the distinction between pediatric and adult types of brainstem gliomas].
abstract: OBJECTIVE
  To study the different expression of miRNA between pediatric and adult types of brainstem gliomas, and to provide the target miRNAs for explore the mechanism and miRNA interference of the malignant progression of pediatric BSG.
  
  
  METHODS
  miRNA expression profiles in orthotopic models which could simulate the BSG heterogeneity were examined by microarray and analyzed to obtain the aberrantly expressed miRNAs. The two types of human BSG tissue were utilized to verify the microarray data by qRT-PCR and in situ hybridization for the putative causative miRNAs.
  
  
  RESULTS
  There were 216 miRNAs detected in both the pediatric BSG group and the adult BSG group, 39 miRNAs to be differential expressed in the pediatric BSG group versus adult group, including 10 up-regulated and 29 down-regulated. qRT-PCR and in situ hybridization indicated good consistency with that of the microarray method.
  
  
  CONCLUSIONS
  Aberrantly expressed miRNA may serve as putative causative involvement of malignant progression of pediatric BSG, thereby might be potentially novel targets for therapy.
authors.affiliation: Department of Neurosurgery, Beijing Sanbo Brain Hospital, 11th Clinical College of Medicine, Capital Medical University, Beijing 100093, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.forename: Hong-wei
authors.initials: HW
authors.lastname: Zhang
authors.forename: An-ling
authors.initials: AL
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.forename: Pei-yu
authors.initials: PY
authors.lastname: Pu
authors.forename: Chang-hong
authors.initials: CH
authors.lastname: Shen
authors.forename: Chun-sheng
authors.initials: CS
authors.lastname: Kang
authors.forename: Chun-jiang
authors.initials: CJ
authors.lastname: Yu
mesh_terms: D000328:Adult; D000367:Age Factors; D000818:Animals; D001933:Brain Stem; D020295:Brain Stem Neoplasms / Q000378:metabolism*; D002648:Child; D004195:Disease Models, Animal; D005260:Female; D020869:Gene Expression Profiling; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization; D035683:MicroRNAs / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats
chemical_list: D035683:MicroRNAs