title: Quantitative analysis of glycoprotein glycans.
abstract: The ability to quantitatively determine changes in the N- and O-linked glycans is an essential component of comparative glycomics. Multiple strategies are available to by which this can be accomplished, including; both label free approaches and isotopic labeling strategies. The focus of this chapter is to describe each of these approaches while providing insight into their strengths and weaknesses, so that glycomic investigators can make an educated choice of the strategy that is best suited for their particular application.
authors.affiliation: Department of Biochemistry, University of Georgia, Athens, GA, USA. orlando@ccrc.uga.edu
authors.forename: Ron
authors.initials: R
authors.lastname: Orlando
mesh_terms: D006023:Glycoproteins / Q000737:chemistry*; D007553:Isotope Labeling; D013058:Mass Spectrometry; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*
chemical_list: D006023:Glycoproteins; D011134:Polysaccharides