title: [Diversity and bioactivity of culturable actinobacteria from animal feces].
abstract: OBJECTIVE
  In order to provide new source for discovering new lead compounds of drugs and other products, the diversity and some bioactivities of culturable actinobacteria in animal feces were studied.
  
  
  METHODS
  Five animals' fecal samples were collected from Yunnan Wild Animal Park. The pure cultures of actinobacteria were isolated from these samples by using 5 different media. The 16S rRNA gene sequences of 119 selected strains were determined; the phylogenetic analysis was carried out; and antimicrobial and anti-tumor activities were determined by using agar diffusion method, tumor cell lines k562and HL60 respectively.
  
  
  RESULTS
  In total 20 genera of actinobacteria from the 5 animals' feces were identified. Many strains inhibited Bacillus subtilis, Staphylococcus lentus, Mycobacterium tuberculosis, Candida albicans and Aspergillus niger. Some strains presented antitumor activities. Some known secondary metabolites and Sannastatin, a novel macrolactam polyketide glycoside with bioactivities, were isolated and identified.
  
  
  CONCLUSIONS
  Fecal actinobacteria are a new potential source for discovering drug lead and other industry products.
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China. jiangyikm@hotmail.com
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
authors.forename: Yanru
authors.initials: Y
authors.lastname: Cao
authors.forename: Li
authors.initials: L
authors.lastname: Han
authors.forename: Rongxian
authors.initials: R
authors.lastname: Jin
authors.forename: Dan
authors.initials: D
authors.lastname: Zheng
authors.forename: Wenxiang
authors.initials: W
authors.lastname: He
authors.forename: Youlong
authors.initials: Y
authors.lastname: Li
authors.forename: Xueshi
authors.initials: X
authors.lastname: Huang
mesh_terms: D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D053822:Ailuridae; D000818:Animals; D000835:Animals, Wild / Q000382:microbiology*; D044822:Biodiversity; D005243:Feces / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011323:Primates; D046010:Tigers; D045949:Viverridae