title: Overexpression, crystallization and preliminary X-ray crystallographic analysis of the phosphotriesterase from Mycobacterium tuberculosis.
abstract: Organophosphates (OPs) are extremely toxic compounds that are used as insecticides or even as chemical warfare agents. Phosphotriesterases (PHPs) are responsible for the detoxification of OPs by catalysing their degradation. Almost 100 PHP structures have been solved to date, yet the crystal structure of the phosphotriesterase from Mycobacterium tuberculosis (mPHP) remains unavailable. This study reports the first crystallization of mPHP. The crystal belonged to space group C222(1), with unit-cell parameters a = 68.03, b = 149.60, c = 74.23 Å, α = β = γ = 90°. An analytical ultracentrifugation experiment suggested that mPHP exists as a dimer in solution, even though one molecule is calculated to be present in the asymmetric unit according to the structural data.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300071, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Ruyi
authors.initials: R
authors.lastname: Chen
authors.forename: Zhe
authors.initials: Z
authors.lastname: Dong
authors.forename: Xin
authors.initials: X
authors.lastname: Li
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D003001:Cloning, Molecular; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000201:enzymology*; D044345:Phosphoric Triester Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D011487:Protein Conformation
chemical_list: D001426:Bacterial Proteins; D044345:Phosphoric Triester Hydrolases