title: Nuclear trafficking of STAT proteins visualized by live cell imaging.
abstract: The ability to observe the dynamic localization of a protein in living cells can provide critical insight to its mode of action and functional molecular interactions. To this purpose, green fluorescent protein (GFP) has served as a powerful tool to tag STAT proteins for microscopic visualization. Live cell imaging with STAT-GFP proteins has contributed to our understanding of signal transduction and the complexities of nuclear transport of STAT proteins. In this report we summarize recent approaches that use GFP-based techniques with live cell imaging to study the mechanisms of STAT nuclear import and export: photoactivation, fluorescence recovery after photobleaching (FRAP), and fluorescence loss in photobleaching (FLIP).
authors.affiliation: Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY, USA.
authors.forename: Velasco
authors.initials: V
authors.lastname: Cimica
authors.forename: Nancy C
authors.initials: NC
authors.lastname: Reich
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000528:radiation effects; D002467:Cell Nucleus / Q000378:metabolism* / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D008027:Light; D057054:Molecular Imaging / Q000379:methods*; D038761:Photobleaching; D050791:STAT Transcription Factors / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D050791:STAT Transcription Factors