title: [Effect of sulfated polysaccharides from brown algae on proliferative and cytotoxic activity of mice splenocytes].
abstract: OBJECTIVE
  Study effect of fucoidans from brown algae on proliferative and cytotoxic activity of mice splenocytes.
  
  
  METHODS
  Proliferative and cytotoxic activity of mice splenocytes in vitro and ex vivo were studied in lymphocyte blast transformation reaction and in cytotoxic MTT-test on K562 human erythroblast leucosis cell line. Microphotography and microscopy were performed by using Axiocam HS photosystem and computer program AxioVision 4 (Germany).
  
  
  RESULTS
  Fucoidans from brown algae Fucus evanescens, Laminaria cichorioides and Laminaria japonica in vitro and ex vivo systems were established to increase proliferative activity of mice splenocytes which is evidenced by an increase of stimulation index. Results of in vitro and ex vivo cytotoxic activity studies demonstrate that fucoidans which are various by chemical structure stimulate activity of NK-cells and facilitate an increase of splenocyte cytotoxic potential level against NK sensitive K562 cell line.
  
  
  CONCLUSIONS
  The data obtained from the study demonstrate an ability of fucoidans to stimulate splenocyte proliferation and NK-cell killer activity, and studies of relation between structure and functions of sulfated polysaccharides facilitate a more detailed understanding of aspects of their mechanism of action on innate immunity system, thus providing the basis for development of new immunobiologic preparations - modifiers/agonists of innate immunity.
authors.forename: I D
authors.initials: ID
authors.lastname: Makarenkova
authors.forename: I B
authors.initials: IB
authors.lastname: Semenova
authors.forename: N K
authors.initials: NK
authors.lastname: Akhmatova
authors.forename: N N
authors.initials: NN
authors.lastname: Besednova
authors.forename: T N
authors.initials: TN
authors.lastname: Zviagintseva
authors.forename: S P
authors.initials: SP
authors.lastname: Ermakova
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D018920:Coculture Techniques; D003601:Cytotoxicity Tests, Immunologic; D044645:Fucus / Q000737:chemistry*; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects; D007155:Immunologic Factors / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D007274:Injections, Intraperitoneal; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D007795:Laminaria / Q000737:chemistry*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008808:Mice, Inbred CBA; D011134:Polysaccharides / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology
chemical_list: D007155:Immunologic Factors; D011134:Polysaccharides