title: [Expression and clinical significance of pyruvate carboxylase in gastric adenocarcinoma].
abstract: OBJECTIVE
  To study the clinical significance of pyruvate carboxylase (PC) expression in gastric adenocarcinoma (GC).
  
  
  METHODS
  Immunohistochemical staining and Western blotting were performed to detect the expression of PC in GC and matched adjacent tumor-free tissues, and statistical analyses were used to determine the relationships of PC expression to patients' parameters such as age, gender, TNM stage, and pathological grade. Using Western blotting, we detected the expression of PC in various gastric cancer cell line and immortalized gastric mucosal epithelium cell line (GES).
  
  
  RESULTS
  Both immunohistochemical staining and Western blotting showed that the expression of PC significantly increased in GC as compared with matched adjacent tumor-free tissues (P<0.01), and PC expression in GC was statistically correlated with various clinical parameters including TNM stage and pathological grade (P<0.05), but not with patient gender and age (P>0.05). What's more, Western blotting revealed that the expressions of PC were markedly higher in all gastric cancer cell lines detected than in GES (P<0.01).
  
  
  CONCLUSIONS
  Increased PC expression is correlated with the malignant progression of GC, which might be a potential target for GC biotherapy.
authors.affiliation: State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an 7 0032, China. kangjianqin01@163.com
authors.forename: Jianqin
authors.initials: J
authors.lastname: Kang
authors.forename: Shanhong
authors.initials: S
authors.lastname: Tang
authors.forename: Guohong
authors.initials: G
authors.lastname: Zhao
authors.forename: Guanghui
authors.initials: G
authors.lastname: Xu
authors.forename: Biaoluo
authors.initials: B
authors.lastname: Wang
authors.forename: Shuhui
authors.initials: S
authors.lastname: Liang
authors.forename: Jie
authors.initials: J
authors.lastname: Ding
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D000328:Adult; D000368:Aged; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011766:Pyruvate Carboxylase / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*
chemical_list: D011766:Pyruvate Carboxylase