title: Detection and cellular localization of phospho-STAT2 in the central nervous system by immunohistochemical staining.
abstract: Phosphorylation of signal transducers and activators of transcription (STATs) indicates their involvement in active signaling. Here we describe immunohistochemical staining procedures for detection and identification of the cellular localization of phospho-STAT2 in the central nervous system (CNS) of mice. The procedures include single and dual/triple labeling immunostaining of phospho-STAT2 in the hippocampus of mice following entorhinal cortex lesion.
authors.affiliation: Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark.
authors.forename: Reza
authors.initials: R
authors.lastname: Khorooshi
authors.forename: Trevor
authors.initials: T
authors.lastname: Owens
mesh_terms: D000818:Animals; D001369:Axons / Q000473:pathology; D018728:Entorhinal Cortex / Q000473:pathology; D005260:Female; D006624:Hippocampus / Q000473:pathology*; D007150:Immunohistochemistry / Q000379:methods*; D051379:Mice; D010750:Phosphoproteins / Q000378:metabolism*; D021381:Protein Transport; D050795:STAT2 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D010750:Phosphoproteins; D050795:STAT2 Transcription Factor