title: The molecular events involved in oligodendrocyte precursor cell proliferation induced by the conditioned medium from b104 neuroblastoma cells.
abstract: The conditioned medium from B104 neuroblastoma cells (B104CM) induces proliferation of oligodendrocyte progenitor cells (OPCs) in vitro. However, the molecular events that occur during B104CM-induced proliferation of OPCs has not been well clarified. In the present study, using OPCs immunopanned from embryonic day 14 Sprague-Dawley rat spinal cords, we explored the activation of several signaling pathways and the expression of several important immediate early genes (IEGs) and cyclins in OPCs in response to B104CM. We found that B104CM can induce OPC proliferation through the activation of the extracellular signal-regulated kinases 1 and 2 (Erk1/2), but not PI3K or p38 MAPK signaling pathways in vitro. The IEGs involved in B104CM-induced OPC proliferation include c-fos, c-jun and Id2, but not c-myc, fyn, or p21. The cyclins D1, D2 and E are also involved in B104CM-stimulated proliferation of OPCs. The activation of Erk results in subsequent expression of IEGs (such as c-fos, c-jun and Id-2) and cyclins (including cyclin D1, D2 and E), which play key roles in cell cycle initiation and OPC proliferation. Collectively, these results suggest that the phosphorylation of Erk1/2 is an important molecular event during OPC proliferation induced by B104CM.
authors.affiliation: Department of Clinical Laboratory Science, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu, 233004, People's Republic of China.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Hu
authors.forename: Xing-Jun
authors.initials: XJ
authors.lastname: Wu
authors.forename: Yi-Fan
authors.initials: YF
authors.lastname: Feng
authors.forename: Gang-Ming
authors.initials: GM
authors.lastname: Xi
authors.forename: Ling-Xiao
authors.initials: LX
authors.lastname: Deng
authors.forename: Zhen-Huan
authors.initials: ZH
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Shen
authors.forename: Jian-Sheng
authors.initials: JS
authors.lastname: Zhou
authors.forename: He-Zuo
authors.initials: HZ
authors.lastname: Lü
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D017781:Genes, Immediate-Early / Q000502:physiology; D009447:Neuroblastoma / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects
chemical_list: D017077:Culture Media, Conditioned; D048049:Extracellular Signal-Regulated MAP Kinases