title: Immobilization of laccase for biotechnology applications.
abstract: Laccase played an important role in the decolorization of wide spectrum dyes as a low-cost and environmentally friendly technology. Laccase was immobilized in alginate beads and immobilization conditions were identified. 25 mg/ml laccase enzyme encapsulation efficiencies of using the prepared bead was calculated as approximately 94%. At the end of the 10 days of storage, the free laccase and immobilized laccase retained about 8.08% and 80.83%, respectively. The decolorization of the dye (Direct Blue 2) was around 86% for immobilized enzyme at 45°C. In the study, compared to the free enzyme, high activity, stable, reusable immobilized enzyme preparation was prepared.
authors.affiliation: Faculty of Science, Biochemistry Department, Ege University, Bornova, İzmir 35100,TÜRKİYE. senay.sanlier@ege.edu.tr
authors.forename: Senay Hamarat
authors.initials: SH
authors.lastname: Sanlıer
authors.forename: Simge
authors.initials: S
authors.lastname: Gider
authors.forename: Alper
authors.initials: A
authors.lastname: Köprülü
mesh_terms: D000464:Alginates / Q000737:chemistry; D001709:Biotechnology / Q000379:methods*; D003116:Color; D004396:Coloring Agents / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000737:chemistry* / Q000378:metabolism*; D013696:Temperature
chemical_list: D000464:Alginates; D004396:Coloring Agents; D004800:Enzymes, Immobilized; D042845:Laccase