title: [Assessment of the usefulness of chromogenic medium for the isolation of Clostridium difficile from the gastrointestinal tract of children with diarrhoea].
abstract: BACKGROUND
  Clostridium difficile is well known as an important cause of nosocomial infection. Laboratory diagnostics have included bacterial culture or more commonly, direct detection of preformed toxin in stool samples using different assays. The aim of this study was to evaluate and compare two selecitve media to isolation of C. difficile from paediatric diarrhoeal stool samples.
  
  
  METHODS
  Fifty nine stool samples, collected from 43 children with diarrhoea, were examined for routine laboratory diagnosis of C. difficile infection. Commercially available tests for detection of A/B toxins of C. difficile were performed. The same stool samples were cultured on two selective media for strain isolation: CLO and CDIFF (bioMerieux S.A., France) and incubated 48h and 24h respectively.
  
  
  RESULTS
  Twenty two samples gave positive results for toxins A/B C. difficile. From 24 samples inoculated on selective media C. difficile strains were cultured: from 8 samples on CLO medium and from 16 samples on CDIFF medium.
  
  
  CONCLUSIONS
  CDIFF medium is more effective for isolation of C. difficile strains from stool samples collected from children with diarrhoea.
authors.affiliation: Katedra i Zakład Mikrobiologii Lekarskiej Warszawski Uniwersytet Medyczny w Warszawie.
authors.forename: Joanna
authors.initials: J
authors.lastname: Kadzielska
authors.forename: Hanna
authors.initials: H
authors.lastname: Pituch
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Banaszkiewicz
authors.forename: Andrzej
authors.initials: A
authors.lastname: Radzikowski
authors.forename: Dorota
authors.initials: D
authors.lastname: Wultańska
authors.forename: Piotr
authors.initials: P
authors.lastname: Obuch-Woszczatyński
authors.forename: Grazyna
authors.initials: G
authors.lastname: Młynarczyk
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D002863:Chromogenic Compounds; D016360:Clostridioides difficile / Q000302:isolation & purification*; D003470:Culture Media; D003967:Diarrhea / Q000382:microbiology*; D004761:Enterocolitis, Pseudomembranous / Q000175:diagnosis / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D041981:Gastrointestinal Tract / Q000382:microbiology; D006801:Humans
chemical_list: D002863:Chromogenic Compounds; D003470:Culture Media