title: [Experience of application of multiplex qPCR for differential diagnostics of intestinal viral infections].
abstract: OBJECTIVE
  Evaluate the effectiveness of multiplex reverse transcription (RT) and polymerase chain reaction with fluorescence detection in real time mode (qPCR) methods for differential detection of 11 groups of intestine viruses (adenoviruses, enteroviruses, polioviruses, hepatitis A and E viruses, group A and C rotaviruses, orthoreoviruses, noroviruses, sapoviruses and astroviruses) in various biological samples.
  
  
  METHODS
  Panels of virus isolates and clinical samples characterized by reference methods were used to evaluate sensitivity of detection of various intestine viruses. Nucleic acids were isolated from study samples and multiplex RT and qPCR were carried out.
  
  
  RESULTS
  Sensitivity of laboratory reagent kit (LRK) when compared with results obtained from reference methods was 100% for rotavirus A, adenovirus, enterovirus and norovirus, 88.9% for hepatitis E virus and 92.3% for hepatitis A virus, and diagnostic specificity - 99.4%. During analysis of 697 clinical samples from patients with acute intestine infection symptoms nucleic acids of various intestine viruses were isolated in 71.7%.
  
  
  CONCLUSIONS
  Multiplex qRT-PCR was shown as an effective method of etiologic diagnostics of an intestine viral infection. Use of LRK was demonstrated to establish etiology of intestine diseases in 63 - 72% and in children with watery diarrhea - in approximately 90% of cases.
authors.forename: A A
authors.initials: AA
authors.lastname: Marova
authors.forename: A S
authors.initials: AS
authors.lastname: Oksanich
authors.forename: A N
authors.initials: AN
authors.lastname: Kaira
authors.forename: E R
authors.initials: ER
authors.lastname: Meskina
authors.forename: E A
authors.initials: EA
authors.lastname: Medvedeva
authors.forename: O E
authors.initials: OE
authors.lastname: Ivanova
authors.forename: A N
authors.initials: AN
authors.lastname: Lukashev
authors.forename: K K
authors.initials: KK
authors.lastname: Kyuregian
authors.forename: M A
authors.initials: MA
authors.lastname: Kalinkina
authors.forename: O V
authors.initials: OV
authors.lastname: Egorova
authors.forename: V V
authors.initials: VV
authors.lastname: Zverev
authors.forename: E V
authors.initials: EV
authors.lastname: Faĭzuloev
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000302:isolation & purification; D000328:Adult; D002648:Child; D003937:Diagnosis, Differential; D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005453:Fluorescence; D006507:Hepatovirus / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007422:Intestines / Q000821:virology*; D008297:Male; D001257:Mamastrovirus / Q000235:genetics / Q000302:isolation & purification; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D029322:Norovirus / Q000235:genetics / Q000302:isolation & purification; D018165:Orthoreovirus / Q000235:genetics / Q000302:isolation & purification; D017955:Poliovirus / Q000235:genetics / Q000302:isolation & purification; D011933:Reagent Kits, Diagnostic; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification; D012426:Russia / Q000453:epidemiology; D029341:Sapovirus / Q000235:genetics / Q000302:isolation & purification; D014777:Virus Diseases / Q000175:diagnosis* / Q000453:epidemiology* / Q000821:virology
chemical_list: D011933:Reagent Kits, Diagnostic