title: HPV genotype distribution according to severity of cervical neoplasia using the Digene HPV genotyping LQ test.
abstract: A new genotyping-based DNA assay (Digene LQ(®)) was developed recently. The primary aim was to assess the distribution of HPV types using this new assay in atypical squamous cells of undeterminate significance (ASCUS). The secondary aim was to correlate the HPV types with the severity of the disease. The study population comprised 376 ASCUS women. The women were all Hybrid Capture II (HCII) positive and were admitted in three European referral gynecology clinics between 2007 and 2010. A colposcopy with histological examination was performed in all these patients. HPV 16 was typed in 40 % of patients, HPV 18 in 7 %, and HPV 31 in 17 %, and 18 % of patients had mixed genotypes. Patients aged over 30 more often had the HPV 16 genotype than patients aged under 30 (29 % vs. 11 %, chi-square test p < 0.001). The risk of cervical intra-epithelial neoplasia of grade 2 or more (CIN2 +) when HPV 18 positive is lower than the probability associated with HPV 16 or HPV 31: 28 % vs. 58 % and 52 %, respectively (chi-square test, p = 0.005 and p = 0.05, respectively). The Digene LQ(®), a new sequence-specific hybrid capture sample preparation, is fast and efficient and allows high-throughput genotyping of 18 HR HPV types by PCR compared to traditional non-sequence-specific sample preparation methods.
authors.affiliation: Laboratoire Alphabio, 23 Rue de Friedland, 13006 Marseille, France. philippe.halfon@alphabio.fr
authors.forename: Philippe
authors.initials: P
authors.lastname: Halfon
authors.forename: Maria Luisa Mateos
authors.initials: ML
authors.lastname: Lindemann
authors.forename: Audrey
authors.initials: A
authors.lastname: Raimondo
authors.forename: Sophie
authors.initials: S
authors.lastname: Ravet
authors.forename: Claire
authors.initials: C
authors.lastname: Camus
authors.forename: Hacène
authors.initials: H
authors.lastname: Khiri
authors.forename: Guillaume
authors.initials: G
authors.lastname: Pénaranda
authors.forename: Mario
authors.initials: M
authors.lastname: Sideri
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Sandri
mesh_terms: D000328:Adult; D000367:Age Factors; D002584:Cervix Uteri / Q000473:pathology / Q000821:virology; D003127:Colposcopy; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005838:Genotype; D052162:Human papillomavirus 16 / Q000235:genetics; D052161:Human papillomavirus 18 / Q000235:genetics; D058952:Human papillomavirus 31 / Q000235:genetics; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000150:complications / Q000821:virology*; D012720:Severity of Illness Index; D002583:Uterine Cervical Neoplasms / Q000209:etiology / Q000473:pathology / Q000821:virology*; D002578:Uterine Cervical Dysplasia / Q000209:etiology / Q000473:pathology / Q000821:virology*
chemical_list: D004279:DNA, Viral