title: One-pot assembly of a hetero-dimeric DNA origami from chip-derived staples and double-stranded scaffold.
abstract: Although structural DNA nanotechnology, and especially scaffolded DNA origami, hold great promise for bottom-up fabrication of novel nanoscale materials and devices, concerns about scalability have tempered widespread enthusiasm. Here we report a single-pot reaction where both strands of double-stranded M13-bacteriophage DNA are simultaneously folded into two distinct shapes that then heterodimerize with high yield. The fully addressable, two-dimensional heterodimer DNA origami, with twice the surface area of standard M13 origami, formed in high yield (81% of the well-formed monomers undergo dimerization). We also report the concurrent production of entire sets of staple strands by a unique, nicking strand-displacement amplification (nSDA) involving reusable surface-bound template strands that were synthesized in situ using a custom piezoelectric inkjet system. The combination of chip-based staple strand production, double-sized origami, and high-yield one-pot assembly markedly increases the useful scale of DNA origami.
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA.
authors.forename: Alexandria N
authors.initials: AN
authors.lastname: Marchi
authors.forename: Ishtiaq
authors.initials: I
authors.lastname: Saaem
authors.forename: Jingdong
authors.initials: J
authors.lastname: Tian
authors.forename: Thomas H
authors.initials: TH
authors.lastname: LaBean
mesh_terms: D004247:DNA / Q000737:chemistry*; D019281:Dimerization; D005559:Formamides / Q000737:chemistry; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D009690:Nucleic Acid Conformation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*
chemical_list: D005559:Formamides; C031066:formamide; D004247:DNA