title: Visualizing the bacterial cell surface: an overview.
abstract: The ultrastructure of bacteria is only accessible by electron microscopy. Our insights into the architecture of cells and cellular compartments such as the envelope and appendages is thus dependent on the progress of preparative and imaging techniques in electron microscopy. Here, I give a short overview of the development and characteristics of methods applied for imaging (components of) the bacterial surface and refer to key investigations and exemplary results. In the beginning of electron microscopy, fixation of biological material and staining for contrast enhancement were the standard techniques. The results from freeze-etching, metal shadowing and from ultrathin-sections of plastic-embedded material shaped our view of the cellular organization of bacteria. The introduction of cryo-preparations, keeping samples in their natural environment, and three-dimensional (3D) electron microscopy of isolated protein complexes and intact cells opened the door to a new dimension and has provided insight into the native structure of macromolecules and the in situ organization of cells at molecular resolution. Cryo-electron microscopy of single particles, together with other methods of structure determination, and cellular cryo-electron tomography will provide us with a quasi-atomic model of the bacterial cell surface in the years to come.
authors.affiliation: Max-Planck-Institut für Biochemie, Martinsried, Germany. engelhar@biochem.mpg.de
authors.forename: Harald
authors.initials: H
authors.lastname: Engelhardt
mesh_terms: D001419:Bacteria / Q000648:ultrastructure*; D001426:Bacterial Proteins / Q000737:chemistry; D002462:Cell Membrane / Q000648:ultrastructure; D020285:Cryoelectron Microscopy; D005614:Freeze Fracturing; D008855:Microscopy, Electron, Scanning
chemical_list: D001426:Bacterial Proteins