title: Tensile stimuli increase nerve growth factor in human dermal fibroblasts independent of tension-induced TGFβ production.
abstract: Human dermal fibroblasts (HDFs) regulate wound-healing processes in human skin, including the regeneration of skin sensory fibres, in response to various mechanical stimuli. Because nerve growth factor (NGF) has an essential role in sensory regeneration, we evaluated the possible association of NGF with mechanical stimulus-dependent cellular responses in HDFs. A cyclic tensile stimulus increased both NGF and transforming growth factor (TGF) β2 production, yet with different gene transcription and signal desensitization profiles. Neutralizing TGFβ with antibodies did not affect the tension-induced NGF upregulation, with significant inhibition of endogenous TGFβ2 transcription. The treatment with LY294002, SP600125 or U0126 hindered the tension-induced TGFβ2 upregulation, although the increase in NGF was regulated only by SP600125 or U0126, indicating the involvement of three signalling kinase pathways in the upregulation of TGFβ2. However, the upregulation of NGF was shown to be independent of PI3K, demonstrating the independent regulation of tension-induced NGF and TGFβ production in HDFs.
authors.forename: Mina
authors.initials: M
authors.lastname: Kim
authors.forename: Dong Wook
authors.initials: DW
authors.lastname: Shin
authors.forename: Hyunjun
authors.initials: H
authors.lastname: Shin
authors.forename: Minsoo
authors.initials: M
authors.lastname: Noh
authors.forename: Jennifer H
authors.initials: JH
authors.lastname: Shin
mesh_terms: D000873:Anthracenes / Q000494:pharmacology; D057134:Antibodies, Neutralizing / Q000494:pharmacology; D002070:Butadienes / Q000494:pharmacology; D002867:Chromones / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D009025:Morpholines / Q000494:pharmacology; D020932:Nerve Growth Factor / Q000235:genetics* / Q000378:metabolism; D009570:Nitriles / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D012867:Skin; D013314:Stress, Mechanical; D014158:Transcription, Genetic / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000276:immunology / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology; D053781:Transforming Growth Factor beta2 / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000873:Anthracenes; D057134:Antibodies, Neutralizing; D002070:Butadienes; D002867:Chromones; D009025:Morpholines; D009570:Nitriles; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D053781:Transforming Growth Factor beta2; C113580:U 0126; C432165:pyrazolanthrone; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; D020932:Nerve Growth Factor