title: Acute skin barrier disruption with repeated tape stripping: an in vivo model for damage skin barrier.
abstract: OBJECTIVE
  To establish a model of standardized acute barrier disruption, investigate the response of normal human to repeated tape stripping, and analyze the change of damaged skin with non-invasive examination techniques for skin, such as TEWL and squamometry.
  
  
  METHODS
  Repeated tape stripping with corneofix was applied on three different anatomical sites; the measurement of TEWL was performed on the baseline and after every 5 strips. Then, the samples of corneofix were analyzed using Visioscan VC98 and squamometry.
  
  
  RESULTS
  The parameter of TEWL and cohesion score show stable change trend. TEWL increased with frequency of stripping and were significantly higher compared with that of baseline on three sites. The results of staining of corneofix showed that the intercorneocyte cohesion is increased with the number of strips, and the more the number of strips, the more the fixation of dye per cell.
  
  
  CONCLUSIONS
  The changes in the skin barrier function of different sites were different after it accepted physical stimulation, the process of damaging skin barrier could be divided into three stages based on the △TEWL. In addition, through stripping the skin of an adult, the in vivo model of damaged skin barrier could be setup.
authors.affiliation: Shanghai Skin Disease Hospital, Clinical School of Anhui Medical University, Shanghai, China. gyanrui@126.com
authors.forename: Yanrui
authors.initials: Y
authors.lastname: Gao
authors.forename: Xuemin
authors.initials: X
authors.lastname: Wang
authors.forename: Shuangyu
authors.initials: S
authors.lastname: Chen
authors.forename: Shuyuan
authors.initials: S
authors.lastname: Li
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Liu
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012867:Skin / Q000293:injuries* / Q000503:physiopathology*; D012869:Skin Absorption; D053832:Surgical Tape / Q000009:adverse effects*; D014870:Water Loss, Insensible; D055815:Young Adult