title: Cytochrome P450 3A mRNA expression along goat and rat gastrointestinal tracts.
abstract: The cytochrome P450 (CYP) 3A family is involved in the elimination processes of almost 50% of commonly used drugs. CYP3A mRNA expressions in goat and rat gastrointestinal tracts in comparison to the liver were investigated using real-time PCR. In goats, the expression of CYP3A-like mRNAs was comparatively higher in the liver than in the gastrointestinal tract. The intestinal expression of CYP3A-like mRNA showed a gradual decrease from the duodenum to the ileum. In rats, the highest CYP3A62 mRNA expression was found in the duodenum followed by the liver. This study provides insights into the contribution of CYP3A enzymes to xenobiotic metabolism, especially in small ruminants such as goats.
authors.affiliation: Department of Public Health, Pharmacology and Toxicology, College of Agriculture and Veterinary Sciences, University of Nairobi, 29053-00625 Nairobi Kenya.
authors.forename: Dominic
authors.initials: D
authors.lastname: Mureithi
authors.forename: Wageh Sobhy
authors.initials: WS
authors.lastname: Darwish
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Ikenaka
authors.forename: Laetitia
authors.initials: L
authors.lastname: Kanja
authors.forename: Mayumi
authors.initials: M
authors.lastname: Ishizuka
mesh_terms: D000818:Animals; D051544:Cytochrome P-450 CYP3A / Q000235:genetics / Q000378:metabolism*; D041981:Gastrointestinal Tract / Q000201:enzymology* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006041:Goats / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D013045:Species Specificity
chemical_list: D012333:RNA, Messenger; D051544:Cytochrome P-450 CYP3A