title: Co-delivery of platelet-derived growth factor (PDGF-BB) and bone morphogenic protein (BMP-2) coated onto heparinized titanium for improving osteoblast function and osteointegration.
abstract: The aim of this study was to improve osteoblast function by delivering two growth factors, PDGF-BB and BMP-2, incorporated onto heparinized titanium (Hep-Ti) substrate. To achieve co-delivery of PDGF-BB and BMP-2, the surface of anodized Ti was immobilized with heparin, and then the two growth factors were coated onto the Hep-Ti surface. Incorporation of the two growth factors onto Hep-Ti was evaluated by SEM and XPS. Incorporated PDGF-BB and BMP-2 were released from the Hep-Ti substrate in a sustained manner. In vitro studies revealed that osteoblasts grown on PDGF-BB- and BMP-2-immobilized Hep-Ti increased ALP activity, calcium deposition, osteocalcin and osteopontin levels as compared to those grown on PDGF-BB alone- or BMP-2 alone-immobilized Hep-Ti. These results suggested that co-delivery of PDGF-BB and BMP-2 using Hep-Ti substrate will be a promising material for the enhancement of osteoblast function and osteointegration.
authors.affiliation: Department of Orthopaedic Surgery and Rare Diseases Institute, Korea University Medical Centre, Guro Hospital, 80 Guro-dong, Guro-gu, Seoul, 152-703, Korea.
authors.forename: Sung Eun
authors.initials: SE
authors.lastname: Kim
authors.affiliation: Department of Orthopaedic Surgery and Rare Diseases Institute, Korea University Medical Centre, Guro Hospital, 80 Guro-dong, Guro-gu, Seoul, 152-703, Korea.
authors.forename: Young-Pil
authors.initials: YP
authors.lastname: Yun
authors.affiliation: Department of Orthopaedic Surgery and Rare Diseases Institute, Korea University Medical Centre, Guro Hospital, 80 Guro-dong, Guro-gu, Seoul, 152-703, Korea.
authors.forename: Jae Yong
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Prosthodontics, College of Dentistry, Younsei University, 134 Sinchon-dong, Seodaemun-gu, Seoul 120-749, Korea.
authors.forename: June-Sung
authors.initials: JS
authors.lastname: Shim
authors.affiliation: Division of Biological Imaging, Chuncheon Centre, Korea Basic Science Institute, 192-1 Hyoja 2-dong, Chuncheon, Gangwon-do, 200-701, Korea.
authors.forename: Kyeongsoon
authors.initials: K
authors.lastname: Park
authors.affiliation: Department of Prosthodontics, College of Dentistry, Younsei University, 134 Sinchon-dong, Seodaemun-gu, Seoul 120-749, Korea.|Department of Prosthodontics, School of Dentistry, Pusan National University, Yang San, 626-787, Korea.
authors.forename: Jung-Bo
authors.initials: JB
authors.lastname: Huh
mesh_terms: D000077214:Becaplermin; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry / Q000494:pharmacology; D002113:Calcification, Physiologic / Q000187:drug effects*; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D006493:Heparin / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D020574:Proto-Oncogene Proteins c-sis / Q000737:chemistry / Q000494:pharmacology; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D020099:Coated Materials, Biocompatible; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D006493:Heparin; D014025:Titanium
keywords: BMP-2; PDGF-BB; bone regeneration; osteoblast; osteointegration; titanium implant