title: [Changes in the subsets of dendritic cells and T cells in peripheral blood of patients with preeclampsia].
abstract: OBJECTIVE
  To investigate the immunoregulation in pathogenesis of pregnancy induced hypertension (PIH) by observing the changes in the subsets of dendritic cells (DC) and T cells in the peripheral blood of patients with preeclampsia.
  
  
  METHODS
  The study included 32 preeclampsia patients, 20 normal pregnancy subjects and 20 non-pregnant healthy controls. We harvested their peripheral blood cells and counted the percentages of myeloid DC (mDC) and plasmacytoid DC (pDC) in whole blood cells, and the numbers of CD4(+); IFN-γ(+);T (Th1), CD4(+); IL-4(+);T (Th2) and CD4(+); IL-17(+);T (Th17) subsets in peripheral blood mononuclear cells by flow cytometry, and worked out the ratio of Th1 to Th2.
  
  
  RESULTS
  The percentage of mDC (0.33±0.12)% and the ratio of mDC/pDC (2.96±1.65) in preeclampsia patients were significantly higher than those in normal pregnancy subjects (P<0.05). The percentage of pDC (0.16±0.13)% was lower than that in normal pregnancy subjects (0.21±0.12)% (P<0.05). The percentages of IFN-γ, IL-4 and IL-17 in preeclampsia patients were (18.67±1.96)%, (1.88±0.51)% and (1.36±0.59)%, respectively, all with statistically significant difference from those of normal late pregnancy (P<0.01). In preeclampsia patients, mDC/pDC ratio was significantly positively correlated with Th1/Th2 (r=0.637, P<0.01) and the percentage of Th17 cells was negatively correlated with pDC cells and positively correlated with mDC/pDC (r=-0.670, 0.772, respectively; P<0.01).
  
  
  CONCLUSIONS
  Preeclampsia patients suffered the changes in dendritic cell subsets and abnormal expressions of Th1, Th2 and Th17, which might be an important reason for immune imbalance in preeclampsia patients.
authors.affiliation: Nantong Maternal and Child Health Hospital, Nantong 226006, China. wangjin2413@sina.com
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Liangxiang
authors.initials: L
authors.lastname: Su
authors.forename: Tianfeng
authors.initials: T
authors.lastname: Zhu
authors.forename: Mi
authors.initials: M
authors.lastname: Shen
mesh_terms: D000328:Adult; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D011225:Pre-Eclampsia / Q000276:immunology*; D011247:Pregnancy; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D058504:Th17 Cells / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism; D055815:Young Adult
chemical_list: D016207:Cytokines