title: Identification of cell wall proteins in the flax (Linum usitatissimum) stem.
abstract: Sequential salt (CaCl2 , LiCl) extractions were used to obtain fractions enriched in cell wall proteins (CWPs) from the stem of 60-day-old flax (Linum usitatissimum) plants. High-resolution FT-ICR MS analysis and the use of recently published genomic data allowed the identification of 11 912 peptides corresponding to a total of 1418 different proteins. Subcellular localization using TargetP, Predotar, and WoLF PSORT led to the identification of 152 putative flax CWPs that were classified into nine different functional classes previously established for Arabidopsis thaliana. Examination of different functional classes revealed the presence of a number of proteins known to be involved in, or potentially involved in cell-wall metabolism in plants. The flax stem cell wall proteome was also compared with transcriptomic data previously obtained on comparable samples. This study represents a major contribution to the identification of CWPs in flax and will lead to a better understanding of cell wall biology in this species.
authors.affiliation: Université de Lille 1 Sciences et Technologies and Protéomique, Modifications Post-traductionnelles et Glycobiologie, IFR 147, Villeneuve d'Ascq, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Day
authors.forename: Stéphane
authors.initials: S
authors.lastname: Fénart
authors.forename: Godfrey
authors.initials: G
authors.lastname: Neutelings
authors.forename: Simon
authors.initials: S
authors.lastname: Hawkins
authors.forename: Christian
authors.initials: C
authors.lastname: Rolando
authors.forename: Caroline
authors.initials: C
authors.lastname: Tokarski
mesh_terms: D002122:Calcium Chloride / Q000737:chemistry; D002473:Cell Wall / Q000737:chemistry; D019597:Flax / Q000737:chemistry*; D013058:Mass Spectrometry; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry; D010940:Plant Proteins / Q000032:analysis / Q000737:chemistry*; D018547:Plant Stems / Q000737:chemistry; D040901:Proteomics / Q000379:methods*
chemical_list: D010446:Peptide Fragments; D010940:Plant Proteins; D002122:Calcium Chloride