title: A new tospovirus causing chlorotic ringspot on Hippeastrum sp. in China.
abstract: A new tospovirus, HCRV 2007-ZDH, was isolated from a Hippeastrum sp. plant displaying necrotic and chlorotic ringspot symptoms in Yunnan province. This virus isolate was characterized based on particle morphology and RNA sequences analyses. Quasi-spherical, enveloped particles measuring about 70-100 nm, typical of tospoviruses, were observed in sap and cells of the infected plants. Transmission studies by inoculating this isolate mechanically to Hippeastrum sp. confirmed that 2007-ZDH is the causal agent of the chlorotic ringspot disease of Hippeastrum sp. The complete sequence of S RNA of 2007-ZDH was 2,744 nucleotides in length, sharing 74.4 % nucleotide identity with Tomato yellow ring virus (TYRV) isolate tomato (AY686718). The S RNA encoded a non-structural protein (NSs) (444 aa, 50.4 kDa) and the nucleocapsid (N) protein (273 aa, 30.1 kDa).The deduced NSs protein shared amino acid identities of 78.6, 76.3, and 74.9 % with that of TYRV, IYSV, and PolRSV, respectively. The deduced N protein shared amino acid identities of 86.1, 84.7, and 70.0 % with that of PolRSV, TYRV, and IYSV, respectively. These results suggest that the chlorotic ringspot virus belongs to a new tospovirus species, for which the name Hippeastrum chlorotic ringspot virus (HCRV) is proposed.
authors.affiliation: Yunnan Key Laboratory of Agricultural Biotechnology, Biotechnology and Genetic Germplasm Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650223, China. dongjhn@126.com
authors.forename: J H
authors.initials: JH
authors.lastname: Dong
authors.forename: Y Y
authors.initials: YY
authors.lastname: Yin
authors.forename: Q
authors.initials: Q
authors.lastname: Fang
authors.forename: J H
authors.initials: JH
authors.lastname: McBeath
authors.forename: Z K
authors.initials: ZK
authors.lastname: Zhang
mesh_terms: D002681:China; D016000:Cluster Analysis; D019668:Liliaceae / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D017865:Tospovirus / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D014771:Virion / Q000648:ultrastructure
chemical_list: D012367:RNA, Viral