title: Development of industrial brewing yeast with low acetaldehyde production and improved flavor stability.
abstract: Higher acetaldehyde concentration in beer is one of the main concerns of current beer industry in China. Acetaldehyde is always synthesized during beer brewing by the metabolism of yeast. Here, using ethanol as the sole carbon source and 4-methylpyrazole as the selection marker, we constructed a new mutant strain with lower acetaldehyde production and improved ethanol tolerance via traditional mutagenesis strategy. European Brewery Convention tube fermentation tests comparing the fermentation broths of mutant strain and industrial brewing strain showed that the acetaldehyde concentration of mutant strain was 81.67 % lower, whereas its resistant staling value was 1.0-fold higher. Owing to the mutation, the alcohol dehydrogenase activity of the mutant strain decreased to about 30 % of the wild-type strain. In the meantime, the fermentation performance of the newly screened strain has little difference compared with the wild-type strain, and there are no safety problems regarding the industrial usage of the mutant strain. Therefore, we suggest that the newly screened strain could be directly applied to brewing industry.
authors.affiliation: Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, China.
authors.forename: Jinjing
authors.initials: J
authors.lastname: Wang
authors.forename: Nan
authors.initials: N
authors.lastname: Shen
authors.forename: Hua
authors.initials: H
authors.lastname: Yin
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Liu
authors.forename: Yongxian
authors.initials: Y
authors.lastname: Li
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
mesh_terms: D000079:Acetaldehyde / Q000378:metabolism*; D001515:Beer / Q000382:microbiology*; D005285:Fermentation / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D000079:Acetaldehyde