title: The role of Necl-5 in the invasive activity of lung adenocarcinoma.
abstract: Nectin-like molecule-5 (Necl-5) is an immunoglobulin-like molecule that was originally identified as a poliovirus receptor and is often upregulated in cancer cells. It has been said that Necl-5 plays a role in not only cell-cell adhesion, but also cell migration, proliferation, and metastasis. In this study, we used a bronchioloalveolar carcinoma (BAC) cell line and fibroblasts to assess the expression of Necl-5 in the development of cancer-stroma communication by using an easy-to-prepare double-layered collagen gel hemisphere (DL-CGH) system that enables visualization of cell migration during invasion. The expression of Necl-5 was higher in BAC cells than in fibroblasts. This tendency didn't change even when the BAC cells were mixed with fibroblasts. To assess the role of Necl-5 in the invasive activity of the BAC cells, we knocked down its expression using RNA interference (RNAi). The invasion assay with DL-CGH revealed that inhibition of Necl-5 expression in the BAC cells was associated with suppressed invasiveness. In addition, Necl-5 knockdown inhibited the movement and proliferation of the BAC cells. Necl-5 expression in lung cancer cells is crucial for their invasiveness in the cancer-stromal interaction, suggesting that Necl-5 could be a favorable molecular target for the suppression of invasiveness in lung adenocarcinoma.
authors.affiliation: Division of Thoracic Surgery, Kobe University Graduate School of Medicine, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Tane
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Maniwa
authors.forename: Daisuke
authors.initials: D
authors.lastname: Hokka
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Tauchi
authors.forename: Wataru
authors.initials: W
authors.lastname: Nishio
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Okita
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yoshimura
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D000077192:Adenocarcinoma of Lung; D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000378:metabolism / Q000473:pathology*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D017154:Stromal Cells / Q000378:metabolism
chemical_list: D009363:Neoplasm Proteins; D034741:RNA, Small Interfering; D011991:Receptors, Virus; C066100:poliovirus receptor