title: [Angiogenesis related gene expression profiles of human endometrial endothelial cells isolated from tissue of endometrial cancer].
abstract: OBJECTIVE
  To identify and verify the different genes expression pattern between human endometrial endothelial cells (HEEC) isolated from endometrial cancer and normal endometrium.
  
  
  METHODS
  Endothelial cells were isolated from 5 patients with endometrial cancer (endometrial cancer group 1) and 5 patients with normal endometria tissue (control group 1) admitted from June to November 2007 in Shandong Cancer Hospital. Global expression patterns of endothelial cells were examined using oligonucleotide microarrays. Tissues from 36 patients with endometrial cancer (endometrial cancer group 2) and 10 normal endometrial tissues (control group 2) admitted from January 2007 to April 2008 were selected to verify the expression of different genes, in which up-regulated genes including ESM1, MMP-10, SPP1 and HMGB1 were tested by quantitative real-time PCR and immunohistochemistry.
  
  
  RESULTS
  Microarray analyses revealed 317 genes that exhibited > 2-fold or < 0.5 differences were identified (including 191 genes up-regulated and 126 down-regulated). Pathway analysis showed that these genes involved cell cycle, cell adhesion molecules, and extracellular matrix-receptor interaction were obviously predominant. Of them, 97 up-regulated genes and 44 down-regulated genes were related to angiogenesis. The mRNA expression of ESM1, MMP-10, SPP1 and HMGB1 in endometrial cancer group 2 were 0.898, 3.890, 1.433 and 1.881, respectively. Positive expression of SPP1, MMP-10, ESM1 and HMGB1 was observed in endometrial cancer group 2. However, the SPP1, ESM1 and HMGB1 was negative expressed in control group 2.
  
  
  CONCLUSIONS
  It shows that there are the different angiogenesis related genes between endometrial cancer and normal endothelium, which will provide insights into the anti-angiogenesis therapy for endometrial cancers.
authors.affiliation: Department of Gynecologic Oncology, Shandong Cancer Hospital, Jinan 250117, China.
authors.forename: Xue-lian
authors.initials: XL
authors.lastname: Du
authors.forename: Xiu-gui
authors.initials: XG
authors.lastname: Sheng
authors.forename: Chun-xiao
authors.initials: CX
authors.lastname: Yan
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.forename: Cong
authors.initials: C
authors.lastname: Wang
authors.forename: Qu-qing
authors.initials: QQ
authors.lastname: Song
authors.forename: Qing-shui
authors.initials: QS
authors.lastname: Li
mesh_terms: D000328:Adult; D016889:Endometrial Neoplasms / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000098:blood supply / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D024243:HMGB1 Protein / Q000235:genetics / Q000378:metabolism; D006801:Humans; D053506:Matrix Metalloproteinase 10 / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D009389:Neovascularization, Pathologic; D020411:Oligonucleotide Array Sequence Analysis; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism; D011509:Proteoglycans / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C101134:ESM1 protein, human; D024243:HMGB1 Protein; D009363:Neoplasm Proteins; D011509:Proteoglycans; D012333:RNA, Messenger; C506895:SPP1 protein, human; D053495:Osteopontin; C511770:MMP10 protein, human; D053506:Matrix Metalloproteinase 10