title: Iodobacter arcticus sp. nov., a psychrotolerant bacterium isolated from meltwater stream sediment of an Arctic glacier.
abstract: Two novel violet-pigmented, Gram-negative, rod-shaped, non-motile bacteria, designated strains M4-16(T) and M4-9, were isolated from sediment from an Arctic glacier. The predominant fatty acids of both strains were C16 : 1ω7c and/or C16 : 1ω6c (summed feature 3), C16 : 0, C14 : 0 and C18 : 1ω7c and/or C18 : 1ω6c (summed feature 8) and both strains contained ubiquinone-8 as the respiratory quinone. The polar lipids consisted of phosphatidylethanolamine, two unidentified phospholipids and one unidentified aminolipid. 16S rRNA gene sequence analysis indicated that strains M4-16(T) and M4-9 were members of the genus Iodobacter and closely related to Iodobacter fluviatilis ATCC 33051(T) with pairwise sequence similarity of 98.9 %. The DNA-DNA relatedness between strains M4-9 and M4-16(T) was 92.5 %, while strains M4-9 and M4-16(T) had DNA-DNA relatedness values of 21.5 and 18.2 %, respectively, with Iodobacter fluviatilis JCM 9044(T). The RAPD-PCR banding patterns of strains M4-9 and M4-16(T) were similar but differed from that of Iodobacter fluviatilis JCM 9044(T). Based on data from the current polyphasic study, strains M4-16(T) and M4-9 represent a novel species of the genus Iodobacter, for which the name Iodobacter arcticus sp. nov. is proposed. The type strain of Iodobacter arcticus is M4-16(T) ( = CIP 1103011(T) = MTCC 11351(T)).
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology Uppal Road, Hyderabad-500 007, India.
authors.forename: T N R
authors.initials: TNR
authors.lastname: Srinivas
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology Uppal Road, Hyderabad-500 007, India.
authors.forename: P
authors.initials: P
authors.lastname: Manasa
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology Uppal Road, Hyderabad-500 007, India.
authors.forename: Z
authors.initials: Z
authors.lastname: Begum
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology Uppal Road, Hyderabad-500 007, India.
authors.forename: B
authors.initials: B
authors.lastname: Sunil
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology Uppal Road, Hyderabad-500 007, India.
authors.forename: B
authors.initials: B
authors.lastname: Sailaja
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology Uppal Road, Hyderabad-500 007, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Singh
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology Uppal Road, Hyderabad-500 007, India.
authors.forename: S
authors.initials: S
authors.lastname: Prasad
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology Uppal Road, Hyderabad-500 007, India.
authors.forename: S
authors.initials: S
authors.lastname: Shivaji
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D046448:Ice Cover / Q000382:microbiology*; D008969:Molecular Sequence Data; D009346:Neisseriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000032:analysis; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8