title: Quantification of protein carbonylation.
abstract: Protein carbonylation is the most commonly used measure of oxidative modification of proteins. It is most often measured spectrophotometrically or immunochemically by derivatizing proteins with the classical carbonyl reagent 2,4 dinitrophenylhydrazine (DNPH). We present protocols for the derivatization and quantification of protein carbonylation with these two methods, including a newly described dot blot with greatly increased sensitivity.
authors.affiliation: Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD, USA.
authors.forename: Nancy B
authors.initials: NB
authors.lastname: Wehr
authors.forename: Rodney L
authors.initials: RL
authors.lastname: Levine
mesh_terms: D000818:Animals; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D006834:Hydrazines / Q000737:chemistry; D006851:Hydrochloric Acid / Q000737:chemistry; D015151:Immunoblotting / Q000379:methods*; D050050:Protein Carbonylation; D051381:Rats; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013053:Spectrophotometry
chemical_list: D005456:Fluorescent Dyes; D006834:Hydrazines; C446799:dinitrophenylhydrazine; D012967:Sodium Dodecyl Sulfate; D006851:Hydrochloric Acid; D004121:Dimethyl Sulfoxide