title: Caryophyllene-rich essential oil of Didymocarpus tomentosa: chemical composition and cytotoxic activity.
abstract: The essential oil from the leaves of Didymocarpus tomentosa was extracted by hydrodistillation and analyzed by GC/FID and GC/MS. Twenty five constituents amounting to 81.6% of the oil were identified. The leaf oil contained 78.7% sesquiterpenes and 2.9% monoterpenes. The leaf essential oil of D. tomentosa is a unique caryophyllene-rich natural source containing beta-caryophyllene, caryophyllene oxide, a-humulene and humulene oxide. The cytotoxic activity of the oil was determined by the BSLT using shrimp larva and the MTT assay using HeLa tumor cell line. The oil showed significant cytotoxic activity with LC50 and IC50 values of 12.26 and 11.4 microg/mL, respectively. This is the first report on the chemical composition and cytotoxic activity of the essential oil of D. tomentosa.
authors.affiliation: Department of PG Studies and Research in Biotechnology, Government Science College, Bangalore, India.
authors.forename: Prameela Javarayi
authors.initials: PJ
authors.lastname: Gowda
authors.forename: Hanumanthaiah
authors.initials: H
authors.lastname: Ramakrishnaiah
authors.forename: Venkatarangaiah
authors.initials: V
authors.lastname: Krishna
authors.forename: Sivakrishna
authors.initials: S
authors.lastname: Narra
authors.forename: Nataraj
authors.initials: N
authors.lastname: Jagannath
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis*; D001156:Artemia; D004354:Drug Screening Assays, Antitumor; D006367:HeLa Cells; D006801:Humans; D019684:Magnoliopsida / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry*; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes / Q000032:analysis*; D018675:Toxicity Tests
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D009822:Oils, Volatile; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; C024714:caryophyllene