title: Enhanced accumulation of phytosterol and triterpene in hairy root cultures of Platycodon grandiflorum by overexpression of Panax ginseng 3-hydroxy-3-methylglutaryl-coenzyme A reductase.
abstract: 3-Hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) catalyzes the rate-limiting step in the mevalonate pathway. To elucidate the functions of HMGR in triterpene biosynthesis, Platycodon grandiflorum was transformed with a construct expressing Panax ginseng HMGR (PgHMGR). We used PCR analysis to select transformed hairy root lines and selected six lines for further investigation. Quantitative real-time PCR showed higher expression levels of HMGR and total platycoside levels (1.5-2.5-fold increase) in transgenic lines than in controls. Phytosterols levels were also 1.1-1.6-fold higher in transgenic lines than in controls. Among these lines, line T7 produced the highest level of total platycosides (1.60 ± 0.2 mg g(-1) dry weight) and α-spinasterol (1.78 ± 0.16 mg g(-1) dry weight). These results suggest that metabolic engineering of P. grandiflorum by Agrobacterium-mediated genetic transformation may enhance production of phytosterols and triterpenoids.
authors.affiliation: Department of Crop Science, College of Agriculture & Life Sciences, Chungnam National University, Yuseong-gu, Daejeon, Republic of Korea.
authors.forename: Yong-Kyoung
authors.initials: YK
authors.lastname: Kim
authors.forename: Jae Kwang
authors.initials: JK
authors.lastname: Kim
authors.forename: Yeon Bok
authors.initials: YB
authors.lastname: Kim
authors.forename: Sanghyun
authors.initials: S
authors.lastname: Lee
authors.forename: Soo-Un
authors.initials: SU
authors.lastname: Kim
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Park
mesh_terms: D000214:Acyl Coenzyme A / Q000235:genetics* / Q000378:metabolism; D029422:Campanulaceae / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D015870:Gene Expression; D005894:Panax / Q000201:enzymology* / Q000235:genetics; D010840:Phytosterols / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000166:cytology / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D014315:Triterpenes / Q000378:metabolism*
chemical_list: D000214:Acyl Coenzyme A; D010840:Phytosterols; D010940:Plant Proteins; D014315:Triterpenes; C008047:3-hydroxy-3-methylglutaryl-coenzyme A