title: A real-time PCR assay for rapid detection and quantification of Exserohilum rostratum, a causative pathogen of fungal meningitis associated with injection of contaminated methylprednisolone.
abstract: A species-specific molecular beacon real-time PCR assay was developed for rapid diagnosis of Exserohilum rostratum infection. As low as 100 fg of E. rostratum DNA can be reliably detected in the presence of 50 ng of human DNA, with a dynamic linear quantification range from 20 ng to 200 fg.
authors.affiliation: Public Health Research Institute Center, UMDNJ-NJMS, Newark, New Jersey, USA.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Ruta
authors.initials: R
authors.lastname: Petraitiene
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Walsh
authors.forename: David S
authors.initials: DS
authors.lastname: Perlin
mesh_terms: D001203:Ascomycota / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015169:Colony Count, Microbial / Q000379:methods*; D004340:Drug Contamination; D006801:Humans; D007267:Injections / Q000009:adverse effects*; D016921:Meningitis, Fungal / Q000175:diagnosis* / Q000382:microbiology; D008775:Methylprednisolone / Q000008:administration & dosage*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D008775:Methylprednisolone