title: Transcriptional, biochemical and histochemical investigation on laccase expression during Tuber melanosporum Vittad. development.
abstract: The cDNAs of Tuber melanosporum laccases (Tmellcc1 and Tmellcc2) have been cloned. From the cloned cDNAs probes were prepared to investigate the expression levels of the Tmellcc1 and Tmellcc2 genes in the free living mycelium (FLM), ectomycorrhizae (ECM) and different developmental stages of fruit body (FB) by quantitative PCR (qPCR). The mRNA expression levels agree with the changes of laccase activities. The histochemical data agree with the qPCR and biochemical results. The highest laccase expression occurs in the ECM, when the host plant roots are invaded by the fungal mycelium.
authors.affiliation: Department of Life, Health & Environmental Sciences, University of L'Aquila, I 67010 Coppito-L'Aquila, Italy.
authors.forename: Osvaldo
authors.initials: O
authors.lastname: Zarivi
authors.forename: Antonella
authors.initials: A
authors.lastname: Bonfigli
authors.forename: Sabrina
authors.initials: S
authors.lastname: Colafarina
authors.forename: Pierpaolo
authors.initials: P
authors.lastname: Aimola
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Ragnelli
authors.forename: Michele
authors.initials: M
authors.lastname: Miranda
authors.forename: Giovanni
authors.initials: G
authors.lastname: Pacioni
mesh_terms: D001203:Ascomycota / Q000201:enzymology* / Q000472:pathogenicity; D031211:Corylus / Q000382:microbiology*; D042845:Laccase / Q000235:genetics / Q000378:metabolism*; D025282:Mycelium / Q000201:enzymology / Q000472:pathogenicity; D018517:Plant Roots / Q000382:microbiology*
chemical_list: D042845:Laccase