title: Approaches for site mapping and quantification of O-linked glycopeptides.
abstract: As a complex post-translational event, the biosynthesis, structures, and functions of O-linked glycans have attracted research interests in various aspects. The recent development of novel technologies for the analysis of glycans and glycoproteins sheds new insights with regard to determining site occupancy, structure-function relationships, and the contributions of O-linked glycosylation to physiological and pathological processes. In this chapter, we refer to several approaches for the structural characterization and quantification of O-linked glycopeptides, with a focus on O-GlcNAc and O-Mannose modified glycoproteins.
authors.affiliation: Complex Carbohydrate Research Center, University of Georgia, Athens, GA, USA.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.forename: Stephanie H
authors.initials: SH
authors.lastname: Stalnaker
authors.forename: Lance
authors.initials: L
authors.lastname: Wells
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism; D000595:Amino Acid Sequence; D001665:Binding Sites; D002846:Chromatography, Affinity; D002853:Chromatography, Liquid; D004229:Dithiothreitol / Q000737:chemistry; D005561:Formates / Q000737:chemistry; D006020:Glycopeptides / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism; D006031:Glycosylation; D008358:Mannose / Q000378:metabolism; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D059748:Proteolysis; D013438:Sulfhydryl Compounds / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D014357:Trypsin / Q000378:metabolism
chemical_list: D005561:Formates; D006020:Glycopeptides; D006023:Glycoproteins; D013438:Sulfhydryl Compounds; C008817:peroxyformic acid; D010744:Phosphoric Monoester Hydrolases; D014357:Trypsin; D008358:Mannose; D010100:Oxygen; D004229:Dithiothreitol; D000117:Acetylglucosamine