title: [Expression and significance of Rab5a and APPL1 in breast cancer].
abstract: OBJECTIVE
  To investigate the expression of Rab5a and APPL1 in breast cancer and fibroma, and analyze their correlation with HER-2 expression, metastasis and development of breast cancer.
  
  
  METHODS
  Rab5a and APPL1 in paraffin embedded tissues of 74 breast carcinomas and 40 breast fibromas were detected by immunohistochemistry. Their relationship with metastasis, pathological grade, and HER-2 expression in breast cancer was determined by statistical analysis.
  
  
  RESULTS
  There was no expression or low expression of Rab5a and APPL1 in the breast fibroma, but the positive expression rate of Rab5a and APPL1 in the breast carcinomas were 91.9% and 83.8%, respectively. No significant difference in expression of Rab5a and APPL1 was found between metastatic and non-metastatic groups, and pathological grade I/II and grade III groups. But Rab5a was overexpressed in HER-2-positive group compared with that in the HER-2-negative group.
  
  
  CONCLUSIONS
  Rab5a and APPL1 are overexpressed in breast cancer, and are positively correlated with the HER-2 expression. These proteins may influence the growth and proliferation of breast cancer cells by HER-2 signal transduction.
authors.affiliation: Department of General Surgery, Minhang Hospital, Ruijin Hospital Group, Shanghai, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.forename: Wei-yan
authors.initials: WY
authors.lastname: Liu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhao
authors.forename: Yun
authors.initials: Y
authors.lastname: Xie
authors.forename: Bao-san
authors.initials: BS
authors.lastname: Han
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism* / Q000473:pathology; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000378:metabolism / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology; D005260:Female; D005350:Fibroma / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008875:Middle Aged; D060787:Neoplasm Grading; D016612:Paraffin Embedding; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D055815:Young Adult; D020696:rab5 GTP-Binding Proteins / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018719:Erb-b2 Receptor Tyrosine Kinases; D020696:rab5 GTP-Binding Proteins; C464801:APPL1 protein, human; C508053:ERBB2 protein, human