title: Selective lead adsorption by recombinant Escherichia coli displaying a lead-binding peptide.
abstract: A highly specific lead-binding peptide ThrAsnThrLeuSerAsnAsn was displayed on Escherichia coli, and lead adsorption characteristics of the recombinant bacteria were investigated. Cell surface-displayed peptide was expressed under the control of an arabinose promoter using outer membrane protein C (OmpC(t)) as an anchoring motif. The optimal induction period and arabinose concentration for the expression of peptide-fused OmpC(t) were determined to be 2 h and 0.001 g/L, respectively. Selective adsorption of Pb(2+) onto recombinant cells was verified with individual or combinatory use of four metal ions, Pb(2+), Ni(2+), Co(2+), and Cu(2+); the amount of bound Pb(2+) onto the biosorbents was significantly higher than the other metal ions. The adsorption isotherm of recombinant cells for Pb(2+) followed the Langmuir isotherm with a maximum adsorption loading (q                 (max)) of 526 μmol/g dry cell weight.
authors.affiliation: School of Chemical Engineering and Bioengineering, University of Ulsan, Ulsan, 680-749, South Korea.
authors.forename: Thuong T L
authors.initials: TT
authors.lastname: Nguyen
authors.forename: Hae Ryong
authors.initials: HR
authors.lastname: Lee
authors.forename: Soon Ho
authors.initials: SH
authors.lastname: Hong
authors.forename: Ji-Ryang
authors.initials: JR
authors.lastname: Jang
authors.forename: Woo-Seok
authors.initials: WS
authors.lastname: Choe
authors.forename: Ik-Keun
authors.initials: IK
authors.lastname: Yoo
mesh_terms: D000327:Adsorption; D003035:Cobalt / Q000378:metabolism; D003300:Copper / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D007854:Lead / Q000378:metabolism*; D009532:Nickel / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*
chemical_list: D010455:Peptides; D007854:Lead; D003035:Cobalt; D003300:Copper; D009532:Nickel