title: Coiled-coil formation on lipid bilayers--implications for docking and fusion efficiency.
abstract: Coiled-coil formation of four different oligopeptides was characterized in solution, on hydrogels, and on membranes by employing circular dichroism spectroscopy, surface plasmon resonance spectroscopy, attenuated total reflection infrared spectroscopy, and ellipsometry. Peptide sequences rich in either glutamic acid (E: E3Cys, i-E3Cys) or lysine (K: K3Cys, i-K3Cys) were used to represent minimal mimics of eukaryotic SNARE motifs. Half of the peptides were synthesized in reverse sequence, so that parallel and antiparallel heptad coiled-coil structures were formed. Either E-peptides or K-peptides were attached covalently to phospholipid anchors via maleimide chemistry, and served as receptors for the recognition of the corresponding binding partners added to solution. Attenuated total reflection infrared spectroscopy of single bilayers confirmed the formation of coiled-coil complexes at the membrane interface. Coiled-coil formation in solution, as compared with association at the membrane surface, displays considerably larger binding constants that are largely attributed to loss of translational entropy at the interface. Finally, the fusogenicity of the various coiled-coil motifs was explored, and the results provide clear evidence that hemifusion followed by full fusion requires a parallel orientation of α-helices, whereas antiparallel oriented coiled-coil motifs display only docking.
authors.affiliation: Institute of Physical Chemistry, Georg August University, Göttingen, Germany.
authors.forename: Gesa
authors.initials: G
authors.lastname: Pähler
authors.forename: Cornelia
authors.initials: C
authors.lastname: Panse
authors.forename: Ulf
authors.initials: U
authors.lastname: Diederichsen
authors.forename: Andreas
authors.initials: A
authors.lastname: Janshoff
mesh_terms: D008051:Lipid Bilayers / Q000737:chemistry*; D008560:Membrane Fluidity; D008561:Membrane Fusion; D008968:Molecular Conformation; D010743:Phospholipids / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D010743:Phospholipids