title: Both Myosin-10 isoforms are required for radial neuronal migration in the developing cerebral cortex.
abstract: During embryonic development of the mammalian cerebral cortex, postmitotic cortical neurons migrate radially from the ventricular zone to the cortical plate. Proper migration involves the correct orientation of migrating neurons and the transition from a multipolar to a mature bipolar morphology. Herein, we report that the 2 isoforms of Myosin-10 (Myo10) play distinct roles in the regulation of radial migration in the mouse cortex. We show that the full-length Myo10 (fMyo10) isoform is located in deeper layers of the cortex and is involved in establishing proper migration orientation. We also demonstrate that fMyo10-dependent orientation of radial migration is mediated at least in part by the netrin-1 receptor deleted in colorectal cancer. Moreover, we show that the headless Myo10 (hMyo10) isoform is required for the transition from multipolar to bipolar morphologies in the intermediate zone. Our study reveals divergent functions for the 2 Myo10 isoforms in controlling both the direction of migration and neuronal morphogenesis during radial cortical neuronal migration.
authors.affiliation: Key Laboratory of Molecular Epigenetics, Ministry of Education, Institute of Genetics and Cytology, Northeast Normal University, Changchun 130021, China.
authors.forename: Xing-Da
authors.initials: XD
authors.lastname: Ju
authors.forename: Ye
authors.initials: Y
authors.lastname: Guo
authors.forename: Nan-Nan
authors.initials: NN
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Lai
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Zhai
authors.forename: Yu-Guang
authors.initials: YG
authors.lastname: Guo
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Zhang
authors.forename: Rang-Juan
authors.initials: RJ
authors.lastname: Cao
authors.forename: Hua-Li
authors.initials: HL
authors.lastname: Yu
authors.forename: Lei
authors.initials: L
authors.lastname: Cui
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Li
authors.forename: Xing-Zhi
authors.initials: XZ
authors.lastname: Wang
authors.forename: Yu-Qiang
authors.initials: YQ
authors.lastname: Ding
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Zhu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002465:Cell Movement / Q000235:genetics*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology*; D000075389:DCC Receptor; D018274:Electroporation; D004622:Embryo, Mammalian; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D066298:In Vitro Techniques; D019394:Ki-67 Antigen / Q000378:metabolism; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009218:Myosins / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D055495:Neurogenesis; D009474:Neurons / Q000502:physiology*; D020033:Protein Isoforms / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000075389:DCC Receptor; C501910:Dcc protein, mouse; D019394:Ki-67 Antigen; D008869:Microtubule-Associated Proteins; C079240:Mtap2 protein, mouse; C409064:Myo10 protein, mouse; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D011956:Receptors, Cell Surface; D014404:Tubulin; D025521:Tumor Suppressor Proteins; C462941:beta3 tubulin, mouse; D049452:Green Fluorescent Proteins; D009218:Myosins
keywords: in utero electroporation; neuronal morphogenesis; radial migration