title: Variations in the oral anaerobic microbial flora in relation to pregnancy.
abstract: BACKGROUND
  Pregnancy gingivitis is a major oral infection. Periodontium acts as a reservoir of inflammatory mediators and sub gingival biofilms of bacteria.
  
  
  OBJECTIVE
  To evaluate the anaerobic oral microbial flora in pregnant women before delivery and after delivery by comparing them with control group.
  
  
  METHODS
  The study group included fifteen cases of pregnant women before and after delivery and healthy non-pregnant women of same age as control group. Sub gingival plaque samples were collected with the help of dentists. The samples were inoculated immediately into Thioglycollate broth (MV010), transported to the laboratory, inoculated on to selective media for anaerobes (Hi-media laboratories) incubated anaerobically (Gas pack).
  
  
  RESULTS
  Prevotella, Tanerella forsythia, Porphyromonas gingivalis and Fusobacterium nucleatum, Veillonella, Peptostreptococcus were isolated.
  
  
  CONCLUSIONS
  The anaerobic bacteria in pregnant women were Prevotella, Tanerella forsythia and Porphyromonas gingivalis. Viellonella and Peptostreptococcus were seen in control group and after delivery. Research suggests that periodontal pathogens may travel the blood stream from the oral cavity to the placenta.
  
  
  CONCLUSIONS
  Pregnancy has significant effect on periodontal tissue. There is a significant alteration of bacterial flora during and after pregnancy. Oral health has to become a part of antenatal care /check up.
authors.affiliation: Professor, Department of Microbiology, Mamata Medical College, Khammam, Andhra Pradesh, India.
authors.forename: Anuradha
authors.initials: A
authors.lastname: Basavaraju
authors.forename: Vijaya
authors.initials: V
authors.lastname: Durga S
authors.forename: B
authors.initials: B
authors.lastname: Vanitha
keywords: Oral anaerobic flora; gingivitis; pregnancy