title: Warming and increased precipitation have differential effects on soil extracellular enzyme activities in a temperate grassland.
abstract: Few studies have conducted the responses of soil extracellular enzyme activities (EEA) to climate change, especially over the long term. In this study, we investigated the six-year responses of soil EEA to warming and increased precipitation in a temperate grassland of northern China at two depths of 0-10 and 10-20 cm. These extracellular enzymes included carbon-acquisition enzymes (β-glucosidase, BG), nitrogen-acquisition enzymes (N-acetylglucosaminidase, NAG; Leucine aminopeptidase, LAP) and phosphorus-acquisition enzymes (acid and alkaline phosphatases). The results showed that warming significantly increased acid phosphatase at the 0-10 cm depth and NAG at the 10-20 cm depth, but dramatically decreased BG and acid phosphatase in the subsurface. In contrast, increased precipitation significantly increased NAG, LAP and alkaline phosphatase in the surface and NAG, LAP and acid phosphatase in the subsurface. There was a significant warming and increased precipitation interaction on BG in the subsurface. Redundancy analysis indicated that the patterns of EEA were mainly driven by soil pH and NH(4)(+)-N and NO(3)(-)-N in the surface, while by NH(4)(+)-N and microbial biomass in the subsurface. Our results suggested that soil EEA responded differentially to warming and increased precipitation at two depths in this region, which may have implications for carbon and nutrient cycling under climate change.
authors.affiliation: Graduate University of Chinese Academy of Sciences, Beijing 100049, China. zhou318@yahoo.com.cn
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Zhou
authors.forename: Chengrong
authors.initials: C
authors.lastname: Chen
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Xu
authors.forename: Hongyan
authors.initials: H
authors.lastname: Han
authors.forename: Linghao
authors.initials: L
authors.lastname: Li
authors.forename: Shiqiang
authors.initials: S
authors.lastname: Wan
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D000135:Acid Phosphatase / Q000378:metabolism; D044602:Cellulases / Q000378:metabolism; D002681:China; D002980:Climate; D057231:Climate Change; D004798:Enzymes / Q000378:metabolism*; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D006109:Poaceae; D012988:Soil Microbiology
chemical_list: D004798:Enzymes; D000135:Acid Phosphatase; D044602:Cellulases; D000118:Acetylglucosaminidase; D007931:Leucyl Aminopeptidase