title: Assessment of polycyclic aromatic hydrocarbon biodegradation potential in mangrove sediment from Don Hoi Lot, Samut Songkram Province, Thailand.
abstract: OBJECTIVE
  To assess the biodegradation potential of mixed polycyclic aromatic hydrocarbons (PAHs) in mangrove sediments.
  
  
  RESULTS
  Sediment microcosms were constructed with sediment collected from Don Hoi Lot, Samut Songkram Province, Thailand, by supplementation with a mixture of acenaphthene, phenanthrene and pyrene. At the end of 8 weeks, low molecular weight PAHs, acenaphthene and phenanthrene were completely degraded. PCR-denaturing gradient gel electrophoresis profile suggests that Marinobacter, Enterobacter and Dethiosulfatibacter play important roles in PAH degradation in mangrove sediment. Furthermore, six PAH-degrading bacteria were isolated consisting of novel phenanthrene-degrading Dyella sp. and Luteibacter sp., phenanthrene-degrading Burkholderia sp., acenaphthene-degrading Alcaligenes sp. and pyrene-degrading Ochrobactrum sp. Moreover, dioxygenase genes could be detected both in sediment microcosms as well as in all of the isolated strains.
  
  
  CONCLUSIONS
  These results demonstrated that indigenous bacteria in the mangrove sediment had the ability to degrade phenanthrene in the presence of mixture PAHs with high efficacy.
  
  
  CONCLUSIONS
  Culture and nonculture methods were combined to assess PAH biodegradation in mangrove sediment. Two novel phenanthrene-degrading bacteria were isolated. Three genera of bacteria that play important roles in PAH degradation were indicated by nonculture approach. Moreover, dioxygenase genes could be detected. This information is useful for further bioremediation of PAH-contaminated mangrove sediments.
authors.affiliation: Bioremediation Research Unit, Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
authors.forename: C
authors.initials: C
authors.lastname: Muangchinda
authors.forename: R
authors.initials: R
authors.lastname: Pansri
authors.forename: W
authors.initials: W
authors.lastname: Wongwongsee
authors.forename: O
authors.initials: O
authors.lastname: Pinyakong
mesh_terms: D000073:Acenaphthenes / Q000737:chemistry; D032407:Avicennia; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D049308:Dioxygenases / Q000235:genetics; D005798:Genes, Bacterial; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology*; D010616:Phenanthrenes / Q000737:chemistry; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D043982:Rhizophoraceae; D013785:Thailand
chemical_list: D000073:Acenaphthenes; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; D012336:RNA, Ribosomal, 16S; C031181:phenanthrene; C030984:pyrene; D049308:Dioxygenases; C042552:acenaphthene