title: Agrobacterium tumefaciens-mediated transformation of the soybean pathogen Phomopsis longicolla.
abstract: To facilitate functional genomics in the soybean pathogen Phomopsis longicolla, we developed a robust Agrobacterium tumefaciens-mediated transformation system that yielded 150-250 transformants per 1×10(6) conidia of P. longicolla. This first report of P. longicolla transformation provides a useful tool for insertional mutagenesis in an increasingly important pathogen of soybean.
authors.affiliation: Crop Genetics Research Unit, United States Department of Agriculture-Agricultural Research Service, Stoneville, MS 38776, USA.
authors.forename: Shuxian
authors.initials: S
authors.lastname: Li
authors.forename: John B
authors.initials: JB
authors.lastname: Ridenour
authors.forename: Hun
authors.initials: H
authors.lastname: Kim
authors.forename: Robert L
authors.initials: RL
authors.lastname: Hirsch
authors.forename: John C
authors.initials: JC
authors.lastname: Rupe
authors.forename: Burton H
authors.initials: BH
authors.lastname: Bluhm
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D001203:Ascomycota / Q000235:genetics*; D018014:Gene Transfer Techniques; D016254:Mutagenesis, Insertional / Q000379:methods; D010935:Plant Diseases / Q000382:microbiology; D013025:Glycine max / Q000382:microbiology; D014170:Transformation, Genetic