title: mRNA delivery to human dendritic cells by recombinant yeast and activation of antigen-specific memory T cells.
abstract: The import of functional nucleic acids like messenger RNA into mammalian cells has proven to be a powerful tool in cell biology and several delivery systems have been described. However, as targeting of particular cell types is a major challenge and RNA vaccination represents a promising means for the induction of cellular immune responses, there is a need for novel delivery systems that permit the introduction of functional messenger RNA to the cytosol of immune cells. Here, we describe a delivery system based on the yeast Saccharomyces cerevisiae that allows the delivery of functional messenger RNA to mammalian antigen-presenting cells such as human dendritic cells. Further, we present a method to prove antigen processing and presentation by stimulation of human autologous T lymphocytes.
authors.affiliation: Molecular and Cell Biology, Saarland University, Saarbrücken, Germany. fb@microbiol.uni-sb.de
authors.forename: Frank
authors.initials: F
authors.lastname: Breinig
authors.forename: Tanja
authors.initials: T
authors.lastname: Breinig
authors.forename: Manfred J
authors.initials: MJ
authors.lastname: Schmitt
mesh_terms: D017951:Antigen Presentation / Q000235:genetics; D000941:Antigens / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D018014:Gene Transfer Techniques; D006801:Humans; D007156:Immunologic Memory; D008213:Lymphocyte Activation / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012441:Saccharomyces cerevisiae; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism
chemical_list: D000941:Antigens; D012333:RNA, Messenger