title: Reconfiguring the architectures of cationic helical polypeptides to control non-viral gene delivery.
abstract: Poly(γ-4-((2-(piperidin-1-yl)ethyl)aminomethyl)benzyl-l-glutamate) (PPABLG), a cationic helical polypeptide, has been recently developed by us as an effective non-viral gene delivery vector. In attempts to elucidate the effect of molecular architecture on the gene delivery efficiencies and thereby identify a potential addition to PPABLG with improved transfection efficiency and reduced cytotoxicity, we synthesized PEG-PPABLG copolymers with diblock, triblock, graft, and star-shaped architectures via a controlled ring-opening polymerization. The PPABLG segment in all copolymers adopted helical structure; all copolymers displayed structure-related cell penetration properties and gene transfection efficiencies. In HeLa and HepG-2 cells, diblock and triblock copolymers exhibited reduced membrane activities and cytotoxicities but uncompromised gene transfection efficiencies compared to the non-PEGylated homo-PPABLG. The graft copolymer revealed lower DNA binding affinity and membrane activity presumably due to the intramolecular entanglement between the grafted PEG segments and charged side chains that led to reduced transfection efficiency. The star copolymer, adopting a spherical architecture with high density of PPABLG, afforded the highest membrane activity and relatively low cytotoxicity, which contributed to its potent gene transfection efficiency that outperformed the non-PEGylated PPABLG and Lipofectamine™ 2000 by 3-5 and 3-134 folds, respectively. These findings provide insights into the molecular design of cationic polymers, especially helical polypeptides towards gene delivery.
authors.affiliation: Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
authors.forename: Lichen
authors.initials: L
authors.lastname: Yin
authors.forename: Ziyuan
authors.initials: Z
authors.lastname: Song
authors.forename: Kyung Hoon
authors.initials: KH
authors.lastname: Kim
authors.forename: Nan
authors.initials: N
authors.lastname: Zheng
authors.forename: Haoyu
authors.initials: H
authors.lastname: Tang
authors.forename: Hua
authors.initials: H
authors.lastname: Lu
authors.forename: Nathan
authors.initials: N
authors.lastname: Gabrielson
authors.forename: Jianjun
authors.initials: J
authors.lastname: Cheng
mesh_terms: D002412:Cations / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D014162:Transfection
chemical_list: D002412:Cations; D010455:Peptides; D011108:Polymers