title: Naked Eye DNA detection: synthesis, characterization and DNA binding studies of a novel azo-guanidine.
abstract: A novel class of azo-guanidine compounds is introduced in this article. The novel compound 2-(2-hydroxyphenyl)-1-(phenylamino)-3-(phenylimino)guanidine (AG) was synthesized and well characterized by using different analytical instrumental techniques like elemental analysis, FTIR, (1)H and (13)C NMR, UV-Visible spectroscopy and cyclic voltammetry. The new compound was found interacting with DNA and shows clear color change in the solution. The AG-DNA complex was qualitatively and quantitatively characterized with UV-Visible spectroscopy and cyclic voltammetry. Electrostatic mode of interaction, clear color change and moderate binding constant (K(b)=10(4)) indicate its potential use as DNA staining agent.
authors.affiliation: Department of Chemistry, Govt. College University Faisalabad, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Jamil
authors.forename: Ataf Ali
authors.initials: AA
authors.lastname: Altaf
authors.forename: Amin
authors.initials: A
authors.lastname: Badshah
authors.lastname: Shafiqullah
authors.forename: Ibrar
authors.initials: I
authors.lastname: Ahmad
authors.forename: Muhammad
authors.initials: M
authors.lastname: Zubair
authors.forename: Saqib
authors.initials: S
authors.lastname: Kemal
authors.forename: Muhammad Irshad
authors.initials: MI
authors.lastname: Ali
mesh_terms: D001391:Azo Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D004396:Coloring Agents / Q000138:chemical synthesis / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D055664:Electrochemical Techniques; D019791:Guanidine / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D009682:Magnetic Resonance Spectroscopy; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D004247:DNA; D019791:Guanidine