title: Complete genome sequence and molecular characterization of thermostable Newcastle disease virus strain TS09-C.
abstract: A thermostable avirulent Newcastle disease virus (NDV) strain TS09-C was developed by serial passage of V4 strain in BHK-21 cells. The complete genome sequence of strain TS09-C was determined and compared with the sequences of other NDV isolates representing different thermostable phenotypes. The TS09-C genome was 15,186 nucleotides long and consisted of eight genes in the order of 3'-NP-P/V/W-M-F-HN-L-5'. High levels of nucleotide and amino acid sequence identity existed among the thermostable NDV isolates. Thermostable strains TS09-C, V4, I-2, and Ulster all belonged to genotype I. The F protein cleavage site of strain TS09-C was (112)G-K-Q-R-R-L(117), with an isolated basic amino acid and a pair of contiguous basic amino acids, differing from that of its parental V4 strain ((112)G-K-Q-G-R-L(117)). Our characterization of the complete genome of thermostable avirulent NDV strain TS09-C may facilitate the development of a thermostable NDV reverse genetic system and further study of the mechanism of thermostability of NDV.
authors.affiliation: Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, 430072, China. wgy_524@163.com
authors.forename: Guoyuan
authors.initials: G
authors.lastname: Wen
authors.forename: Yu
authors.initials: Y
authors.lastname: Shang
authors.forename: Jing
authors.initials: J
authors.lastname: Guo
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Huabin
authors.initials: H
authors.lastname: Shao
authors.forename: Qingping
authors.initials: Q
authors.lastname: Luo
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Hongling
authors.initials: H
authors.lastname: Wang
authors.forename: Guofu
authors.initials: G
authors.lastname: Cheng
mesh_terms: D000818:Animals; D002460:Cell Line; D016000:Cluster Analysis; D006224:Cricetinae; D016679:Genome, Viral; D005838:Genotype; D008969:Molecular Sequence Data; D009522:Newcastle disease virus / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012692:Serial Passage
chemical_list: D012367:RNA, Viral