title: In-vitro platelet hyperaggregation and hypersecretion associated with the use of fish oil in healthy children.
abstract: OBJECTIVE
  The aim of this study is to evaluate how fish oil supplementation in children affects platelet function tests in vitro.
  
  
  METHODS
  The study included 62 children (20 healthy children without any medications and 42 healthy children who volunteered to take fish oil supplementation) aged between 2 and 12 years. In the group of children taking fish oil supplementation, the baseline, fourth week, and eighth week values for platelet function tests were obtained.
  
  
  RESULTS
  In the platelet aggregation tests induced by high dose of ADP after 8 weeks, the values were significantly higher compared with the values measured before the use of fish oil. The fish oil-supplemented group's values showed an increase in the fourth-week measurements compared with the control group and the baseline measurements in terms of platelet secretion test induced by collagen, standard dose of thrombin, and high-dose thrombin. Platelet secretion tests induced by standard dose of ADP at the end of the eighth week showed an increase compared with baseline test values.
  
  
  CONCLUSIONS
  This study was done in in-vitro conditions wherein the platelet function in the pediatric age group was analyzed and it was found that eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) present in fish oil especially exhibit in-vitro hyperaggregation and increase the secretion of platelets. As a result of this, we consider that it is necessary to be careful while using fish oil supplementation in children as an antithrombotic agent and for a variety of other indications.
authors.affiliation: Department of Pediatric Hematology, Ankara Training and Research Hospital; Director of Blood Bank, Therapeutic Apheresis and Hematology Laboratories , Ankara , Turkey.
authors.forename: Bulent
authors.initials: B
authors.lastname: Alioglu
authors.forename: Tugba
authors.initials: T
authors.lastname: Zengin
authors.forename: Nermin
authors.initials: N
authors.lastname: Dindar
authors.forename: Ayse Esra
authors.initials: AE
authors.lastname: Tapci
authors.forename: Yildiz
authors.initials: Y
authors.lastname: Dallar
mesh_terms: D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D003094:Collagen / Q000494:pharmacology; D019587:Dietary Supplements; D005260:Female; D005395:Fish Oils / Q000008:administration & dosage*; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects*; D010979:Platelet Function Tests / Q000379:methods
chemical_list: D005395:Fish Oils; D003094:Collagen