title: Co-immunoprecipitation protocol to investigate cytokine receptor-associated proteins, e.g., Janus kinases or other associated signaling proteins.
abstract: Jak binding to cytokine receptors has been shown to be a complex and tight interaction. When studying loss-of-function or gain-of-function mutants of the Jaks or cytokine receptors it is often necessary to know if a certain mutant still associates correctly in the context of the signaling complex. The standard technique to show interaction of Jaks with cytokine receptors or other signalling molecules is Co-immunoprecipitation. Here we describe our protocol and discuss different pitfalls that can be encountered during the procedure.
authors.affiliation: Life Sciences Research Unit, University of Luxembourg, Luxembourg, UK, claude.haan@uni.lu.
authors.forename: Claude
authors.initials: C
authors.lastname: Haan
authors.forename: Serge
authors.initials: S
authors.lastname: Haan
mesh_terms: D015153:Blotting, Western; D002457:Cell Extracts; D045744:Cell Line, Tumor; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D053612:Janus Kinases / Q000302:isolation & purification* / Q000378:metabolism*; D018121:Receptors, Cytokine / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D002457:Cell Extracts; D018121:Receptors, Cytokine; D053612:Janus Kinases