title: Silibinin in vitro protects A549 cells from Staphylococcus aureus-mediated injury and in vivo alleviates the lung injury of staphylococcal pneumonia.
abstract: In this study, hemolysis, Western blot, and real-time RT-PCR assays were performed to evaluate silibinin's activity against S. aureus α-toxin secretion. In addition, live/dead cell staining and lactate dehydrogenase activity assays were introduced to examine the influence of silibinin on α-toxin-induced cell injury in human alveolar epithelial cells. Furthermore, we tested the influence of silibinin on S. aureus pneumonia in a mouse model. We show that silibinin inhibits the expression of α-toxin in a dose-dependent manner and alleviates α-toxin-induced lung cell injury. The IC50 of silibinin that inhibits the hemolytic activity of S. aureus was 14.33 µg/mL for strain 8325-4. Moreover, this compound provides effective protection on the lung injury of staphylococcal pneumonia.
authors.affiliation: College of Quartermaster Technology, Jilin University, Changchun, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Dong
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Dai
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Wang
authors.forename: Hongen
authors.initials: H
authors.lastname: Li
authors.forename: Chongjian
authors.initials: C
authors.lastname: Lu
authors.forename: Wei
authors.initials: W
authors.lastname: Tan
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Gao
authors.forename: Xuming
authors.initials: X
authors.lastname: Deng
authors.forename: Shijin
authors.initials: S
authors.lastname: Bu
authors.forename: Xiaodi
authors.initials: X
authors.lastname: Niu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D001427:Bacterial Toxins / Q000037:antagonists & inhibitors* / Q000633:toxicity; D015153:Blotting, Western; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005260:Female; D006460:Hemolysin Proteins / Q000037:antagonists & inhibitors* / Q000633:toxicity; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D008168:Lung / Q000166:cytology / Q000187:drug effects / Q000473:pathology; D055370:Lung Injury / Q000382:microbiology / Q000517:prevention & control*; D051379:Mice; D008826:Microbial Sensitivity Tests; D011023:Pneumonia, Staphylococcal / Q000188:drug therapy* / Q000473:pathology; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077385:Silybin; D012838:Silymarin / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D012838:Silymarin; C021091:staphylococcal alpha-toxin; D000077385:Silybin