title: Preliminary crystallographic analysis of a polyadenylate synthase from Megavirus.
abstract: Megavirus chilensis, a close relative of the Mimivirus giant virus, is also the most complex virus sequenced to date, with a 1.26 Mb double-stranded DNA genome encoding 1120 genes. The two viruses share common regulatory elements such as a peculiar palindrome governing the termination/polyadenylation of viral transcripts. They also share a predicted polyadenylate synthase that presents a higher than average percentage of residue conservation. The Megavirus enzyme Mg561 was overexpressed in Escherichia coli, purified and crystallized. A 2.24 Å resolution MAD data set was recorded from a single crystal on the ID29 beamline at the ESRF.
authors.affiliation: Information Génomique et Structurale, IGS UMR7256, CNRS, Aix-Marseille Université, IMM, FR3479, 163 Avenue de Luminy - case 934, 13288 Marseille CEDEX 09, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Lartigue
authors.forename: Sandra
authors.initials: S
authors.lastname: Jeudy
authors.forename: Lionel
authors.initials: L
authors.lastname: Bertaux
authors.forename: Chantal
authors.initials: C
authors.lastname: Abergel
mesh_terms: D001483:Base Sequence; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray; D056686:Mimiviridae / Q000201:enzymology*; D008969:Molecular Sequence Data; D011062:Polynucleotide Adenylyltransferase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D011487:Protein Conformation; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D014764:Viral Proteins; D011062:Polynucleotide Adenylyltransferase