title: [Inhibitory effect of adenovirus-mediated D2S gene plus bromocriptine therapy on primary cultures of human non-functioning pituitary adenoma cells].
abstract: OBJECTIVE
  To explore the inhibitory effect of non-functioning pituitary adenoma (NFPA) cells after a combined treatment of adenovirus mediated D2S gene and bromocriptine in vitro.
  
  
  METHODS
  Adenovirus containing dopamine 2 receptor short isoform (D2S) gene was used to infect NFPA cells. The transfection of D2S gene into NFPA cells was confirmed by immunofluorescence. And cell apoptosis of infected cells treated by bromocriptine was evaluated with CCK-8 assay in vitro.
  
  
  RESULTS
  When D2S gene transfection and bromocriptine was used in combination, the survival rate of NFPA cells significantly decreased (40 ± 5)% versus the control group (97 ± 5)% and the pAd-EGFP transfection combined bromocriptine treatment group (90 ± 9)% (P < 0.05).
  
  
  CONCLUSIONS
  The combined treatment of adenovirus-mediated D2S gene and bromocriptine can effectively induce the apoptosis of NFPA cells on primary culture and increase the sensitivity of NFPA to dopamine agonist.
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Wenzhou Medical College, China.
authors.forename: Zhen-kun
authors.initials: ZK
authors.lastname: Xiong
authors.forename: Wei-qiang
authors.initials: WQ
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Zhe-bao
authors.initials: ZB
authors.lastname: Wu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Yun-xiang
authors.initials: YX
authors.lastname: Chen
authors.forename: Zhi-peng
authors.initials: ZP
authors.lastname: Su
authors.forename: Jin-sen
authors.initials: JS
authors.lastname: Wu
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D017209:Apoptosis / Q000187:drug effects*; D001971:Bromocriptine / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D010911:Pituitary Neoplasms / Q000473:pathology; D017448:Receptors, Dopamine D2 / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D017448:Receptors, Dopamine D2; D001971:Bromocriptine