title: Monitoring nutrient signaling through the longevity protein p66(SHC¹).
abstract: Nutrient availability and nutrient-dependent biochemical signals represent major determinants of cellular senescence and organismal aging. The present chapter describes simple procedures to reliably evaluate the response of cultured cell to nutrients through the longevity protein p66(SHC1) and the mTOR/S6K cascade, which might be used to study cellular senescence and its chemical modulation by pharmaceutical agents in vitro.
authors.affiliation: Clinical Chemistry, Laboratory and Endocrinology Unit, Departement of Laboratory Medicine, Azienda Ospedaliera ASMN, Istituto di Ricovero e Cura a Carattere Scientifico, Reggio Emilia, Italy.
authors.forename: Sofia Chiatamone
authors.initials: SC
authors.lastname: Ranieri
authors.forename: Giovambattista
authors.initials: G
authors.lastname: Pani
mesh_terms: D000375:Aging; D004586:Electrophoresis; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006801:Humans; D008136:Longevity; D010766:Phosphorylation; D055765:Shc Signaling Adaptor Proteins / Q000172:deficiency / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015398:Signal Transduction; D000071425:Src Homology 2 Domain-Containing, Transforming Protein 1; D013194:Staining and Labeling; D014162:Transfection
chemical_list: C528024:SHC1 protein, human; D055765:Shc Signaling Adaptor Proteins; D000071425:Src Homology 2 Domain-Containing, Transforming Protein 1