title: Monitoring axonal and somatodendritic dopamine release using fast-scan cyclic voltammetry in brain slices.
abstract: Brain dopamine pathways serve wide-ranging functions including the control of movement, reward, cognition, learning, and mood. Consequently, dysfunction of dopamine transmission has been implicated in clinical conditions such as Parkinson's disease, schizophrenia, addiction, and depression. Establishing factors that regulate dopamine release can provide novel insights into dopaminergic communication under normal conditions, as well as in animal models of disease in the brain. Here we describe methods for the study of somatodendritic and axonal dopamine release in brain slice preparations. Topics covered include preparation and calibration of carbon-fiber microelectrodes for use with fast-scan cyclic voltammetry, preparation of midbrain and forebrain slices, and procedures of eliciting and recording electrically evoked dopamine release from in vitro brain slices.
authors.affiliation: Departments of Neurosurgery and Physiology & Neuroscience, New York University School of Medicine, New York, NY, USA. Jyotiben.Patel@nyumc.org
authors.forename: Jyoti C
authors.initials: JC
authors.lastname: Patel
authors.forename: Margaret E
authors.initials: ME
authors.lastname: Rice
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism*; D001921:Brain / Q000166:cytology*; D002138:Calibration; D002244:Carbon / Q000737:chemistry; D000077482:Carbon Fiber; D004210:Dissection; D004298:Dopamine / Q000378:metabolism*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D008636:Mesencephalon / Q000166:cytology; D051379:Mice; D008839:Microelectrodes; D008867:Microtomy; D013569:Synapses / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000077482:Carbon Fiber; D002244:Carbon; D004298:Dopamine