title: Rapid determination of isoamyl nitrite in pharmaceutical preparations by flow injection analysis with on-line UV irradiation and luminol chemiluminescence detection.
abstract: Isoamyl nitrite is used as a therapeutic reagent for cardiac angina and as an antidote for cyanide poisoning, but it is abused because of its euphoric properties. Therefore, a method to determine isoamyl nitrite is required in many fields, including pharmaceutical and forensic studies. In this study, a simple, rapid and sensitive method for the determination of isoamyl nitrite was developed using a flow injection analysis system equipped with a chemiluminescence detector and on-line photoreactor. This method is based on on-line ultraviolet irradiation of isoamyl nitrite and subsequent luminol chemiluminescence detection without the addition of an oxidant. A linear standard curve was obtained up to 1.0 μM of isoamyl nitrite with a detection limit (blank + 3SD) of 0.03 μM. The method was successfully applied to determine isoamyl nitrite content in pharmaceutical preparations.
authors.affiliation: Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, 852-8521, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Kishikawa
authors.forename: Naoko
authors.initials: N
authors.lastname: Kondo
authors.forename: Abena
authors.initials: A
authors.lastname: Amponsaa-Karikari
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Kodamatani
authors.forename: Kaname
authors.initials: K
authors.lastname: Ohyama
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Nakashima
authors.forename: Shigeo
authors.initials: S
authors.lastname: Yamazaki
authors.forename: Naotaka
authors.initials: N
authors.lastname: Kuroda
mesh_terms: D000680:Amyl Nitrite / Q000031:analogs & derivatives* / Q000032:analysis; D002138:Calibration; D049449:Luminescence; D008165:Luminol / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D004364:Pharmaceutical Preparations; D008165:Luminol; C048958:isoamyl nitrite; D000680:Amyl Nitrite
keywords: alkyl nitrite; flow injection analysis; luminol chemiluminescence; photochemical reaction