title: Genetic modification of primate amniotic fluid-derived stem cells produces pancreatic progenitor cells in vitro.
abstract: Insulin therapy for type 1 diabetes does not prevent serious long-term complications including vascular disease, neuropathy, retinopathy and renal failure. Stem cells, including amniotic fluid-derived stem (AFS) cells - highly expansive, multipotent and nontumorigenic cells - could serve as an appropriate stem cell source for β-cell differentiation. In the current study we tested whether nonhuman primate (nhp)AFS cells ectopically expressing key pancreatic transcription factors were capable of differentiating into a β-cell-like cell phenotype in vitro. nhpAFS cells were obtained from Cynomolgus monkey amniotic fluid by immunomagnetic selection for a CD117 (c-kit)-positive population. RT-PCR for endodermal and pancreatic lineage-specific markers was performed on AFS cells after adenovirally transduced expression of PDX1, NGN3 and MAFA. Expression of MAFA was sufficient to induce insulin mRNA expression in nhpAFS cell lines, whereas a combination of MAFA, PDX1 and NGN3 further induced insulin expression, and also induced the expression of other important endocrine cell genes such as glucagon, NEUROD1, NKX2.2, ISL1 and PCSK2. Higher induction of these and other important pancreatic genes was achieved by growing the triply infected AFS cells in media supplemented with a combination of B27, betacellulin and nicotinamide, as well as culturing the cells on extracellular matrix-coated plates. The expression of pancreatic genes such as NEUROD1, glucagon and insulin progressively decreased with the decline of adenovirally expressed PDX1, NGN3 and MAFA. Together, these experiments suggest that forced expression of pancreatic transcription factors in primate AFS cells induces them towards the pancreatic lineage.
authors.affiliation: Wake Forest Institute for Regenerative Medicine, Winston Salem, N.C. 27157, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.forename: David L
authors.initials: DL
authors.lastname: Mack
authors.forename: J Koudy
authors.initials: JK
authors.lastname: Williams
authors.forename: Sayed-Hadi
authors.initials: SH
authors.lastname: Mirmalek-Sani
authors.forename: Emily
authors.initials: E
authors.lastname: Moorefield
authors.forename: So-Young
authors.initials: SY
authors.lastname: Chun
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Diego
authors.initials: D
authors.lastname: Lorenzetti
authors.forename: Mark
authors.initials: M
authors.lastname: Furth
authors.forename: Anthony
authors.initials: A
authors.lastname: Atala
authors.forename: Shay
authors.initials: S
authors.lastname: Soker
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000096:biosynthesis / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D015870:Gene Expression; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D008252:Macaca fascicularis; D051473:Maf Transcription Factors, Large / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D009687:Nuclear Proteins; D010179:Pancreas / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D015534:Trans-Activators / Q000096:biosynthesis / Q000235:genetics; D014157:Transcription Factors
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins; D051473:Maf Transcription Factors, Large; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C485159:MAFA protein, human; C485160:Mafa protein, mouse; C498695:NEUROG3 protein, human; C000715631:NKX2-2 protein, human; C000715627:Nkx2-2 protein, mouse; C084010:pancreatic and duodenal homeobox 1 protein