title: Species-specific challenges in dog cloning.
abstract: Somatic cell nuclear transfer (SCNT) is now an established procedure used in cloning of several species. SCNT in dogs involves multiple steps including the removal of the nuclear material, injection of a donor cell, fusion, activation of the reconstructed oocytes and finally transfer to a synchronized female recipient. There are therefore many factors that contribute to cloning efficiency. By performing a retrospective analysis of 2005-2012 published papers regarding dog cloning, we define the optimum procedure and summarize the specific feature for dog cloning.
authors.affiliation: Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
authors.forename: G A
authors.initials: GA
authors.lastname: Kim
authors.forename: H J
authors.initials: HJ
authors.lastname: Oh
authors.forename: J E
authors.initials: JE
authors.lastname: Park
authors.forename: M J
authors.initials: MJ
authors.lastname: Kim
authors.forename: E J
authors.initials: EJ
authors.lastname: Park
authors.forename: Y K
authors.initials: YK
authors.lastname: Jo
authors.forename: G
authors.initials: G
authors.lastname: Jang
authors.forename: M K
authors.initials: MK
authors.lastname: Kim
authors.forename: H J
authors.initials: HJ
authors.lastname: Kim
authors.forename: B C
authors.initials: BC
authors.lastname: Lee
mesh_terms: D000818:Animals; D019976:Cloning, Organism / Q000379:methods / Q000662:veterinary*; D004285:Dogs / Q000235:genetics* / Q000502:physiology*; D004624:Embryo Transfer; D053652:Nuclear Transfer Techniques / Q000662:veterinary*; D009865:Oocytes; D013045:Species Specificity