title: Prevalence and characterization of genotypic diversity of Haemophilus parasuis isolates from southern China.
abstract: From September 2008 to December 2010, 112 Haemophilus parasuis strains were isolated from 536 pigs with clinical signs of Glässer's disease in South China, for a frequency of 21%. The 112 strains were subjected to serovar analysis by gel diffusion (GD) and indirect hemagglutination (IHA) tests and to genotype analysis by means of pulsed-field gel electrophoresis (PFGE). With a combination of the GD and IHA results, serovars 5 and 4 were found to be the most prevalent, at 23% and 17%, respectively, followed by serovars 2 (8%), 15 (7%), 13 (6%), and 12 (5%); 20% of the strains were nontypeable. The 112 strains were genetically diverse, with 85 genotypes identified (discriminatory index 0.992). The 89 typeable isolates belonged to 15 H. parasuis serovars displaying 63 different PFGE profiles. The 23 nontypeable strains displayed 22 different PFGE profiles. These findings confirmed that 15 serovars and diverse genotypes of H. parasuis were widely distributed in southern China.
authors.affiliation: Key Laboratory of Animal Disease Control and Prevention of the Ministry of Agriculture, Key Laboratory of Zoonoses Prevention and Control of Guangdong, College of Veterinary Medicine, South China Agricultural University, No. 483 Wushan Road, Tianhe District, Guangzhou, Guangdong Province, China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Zhang
authors.forename: Chenggang
authors.initials: C
authors.lastname: Xu
authors.forename: Lili
authors.initials: L
authors.lastname: Guo
authors.forename: Haiyan
authors.initials: H
authors.lastname: Shen
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Deng
authors.forename: Changwen
authors.initials: C
authors.lastname: Ke
authors.forename: Bixia
authors.initials: B
authors.lastname: Ke
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Ang
authors.initials: A
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Ren
authors.forename: Ming
authors.initials: M
authors.lastname: Liao
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation; D006192:Haemophilus Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D044137:Haemophilus parasuis / Q000145:classification* / Q000235:genetics*; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology*