title: New strategy for enhancing the therapeutic index of Fungizone(®).
abstract: The aim of this study was to enhance the therapeutic index of Fungizone(®). For this reason we have prepared stock solutions of Fungizone(®) under different conditions of pHs (from 4.8 to 10.8) and temperatures (unheated solutions and heated ones during 20 min at 70 °C). Then we have evaluated the effect of those antifungal solutions on human red blood cells coming from one healthy donor, as well as on the dormancy of Candida albicans strain. The obtained results show that after exposure to antifungal stock solution prepared at pH 5.4 and heated during 20 min at 70 °C, the rate of hemolysis was largely reduced (only 5% of hemolysis). On the other hand, it is noticed the yeast regrowth after 26 h whatever is the temperature or the pH of the stock solution. However at pH 5.4, the growth of C. albicans is inhibited at 75% in comparison with the one obtained with the conventional Fungizone(®) after 30 h of culture where we observed 100% of growth. So, stock solution of Fungizone(®) prepared at pH 5.4 and heated 20 min at 70 °C seems to be a good strategy for enhancing the therapeutic index of Fungizone(®).
authors.affiliation: Laboratoire Antibiotiques Antifongiques, Physicochimie Synthèse et Activité Biologique, Université de Tlemcen, Tlemcen, Algeria. lamiabio@yahoo.fr
authors.forename: L
authors.initials: L
authors.lastname: Belkherroubi-Sari
authors.forename: H
authors.initials: H
authors.lastname: Adida
authors.forename: A
authors.initials: A
authors.lastname: Seghir
authors.forename: Z
authors.initials: Z
authors.lastname: Boucherit
authors.forename: K
authors.initials: K
authors.lastname: Boucherit
mesh_terms: D000666:Amphotericin B / Q000737:chemistry / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012996:Solutions; D013696:Temperature
chemical_list: D000935:Antifungal Agents; D012996:Solutions; D000666:Amphotericin B