title: Optimization of lipase-catalyzed synthesis of fructose stearate using response surface methodology.
abstract: In this study, immobilized lipase-catalyzed esterification reaction between fructose and stearic acid was examined for the synthesis of a useful compound, fructose stearate, using response surface methodology. The increase of water content in the reaction medium was the negative effect while the increase in initial stearic acid/fructose molar ratio was the greatest positive effect on the yield. The highest fructose stearate yield was obtained as 65% in tert-butanol. The product yield was enhanced in 1-butyl-3-methylimidazolium trifluoromethanesulfonate obtained as 74% under the optimized conditions. The spectroscopic and elemental analysis methods showed that the esterification reaction is regioselective and the product is fructose monostearate.
authors.affiliation: Department of Chemistry, The Faculty of Sciences and Letters, University of Cukurova , Adana , Turkey.
authors.forename: Seyhan
authors.initials: S
authors.lastname: Tükel
authors.forename: Pınar Basım
authors.initials: PB
authors.lastname: Sahin
authors.forename: Deniz
authors.initials: D
authors.lastname: Yildirim
mesh_terms: D000704:Analysis of Variance; D004800:Enzymes, Immobilized / Q000378:metabolism; D004951:Esterification; D005632:Fructose / Q000138:chemical synthesis*; D007093:Imidazoles; D008049:Lipase / Q000378:metabolism; D008698:Mesylates; D015233:Models, Statistical; D055550:Protein Stability; D013057:Spectrum Analysis; D013228:Stearates / Q000138:chemical synthesis*; D013229:Stearic Acids / Q000138:chemical synthesis*; D020002:tert-Butyl Alcohol / Q000138:chemical synthesis*
chemical_list: C532403:1-butyl-3-methylimidazolium trifluoromethanesulfonate; D004800:Enzymes, Immobilized; D007093:Imidazoles; D008698:Mesylates; D013228:Stearates; D013229:Stearic Acids; D005632:Fructose; C031183:stearic acid; D008049:Lipase; D020002:tert-Butyl Alcohol