title: An effective and rapid determination by MALDI/TOF/TOF of methionine sulphoxide content of ApoA-I in type 2 diabetic patients.
abstract: Increased oxidation of low density lipoprotein (LDL) is characteristic of atherosclerosis. In this frame, high density lipoproteins (HDL) play an important role, being able to remove lipid peroxides (LPOs) and cholesterol from oxidized LDL, so exhibiting a protective role against atherosclerosis. A wide range of reactive compounds lead to the oxidation of methionine (Met) residues with the formation of methionine sulphoxide (MetO) in apolipoprotein A-I (ApoA-I). Consequently, the determination of MetO level can give both an evaluation of oxidative stress and the reduced capability of ApoA-I in LPOs and cholesterol transport. For these reasons, the development of analytical methods able to determine the MetO level is surely of interest, and we report here the results obtained by MALDI mass spectrometry.
authors.affiliation: CNR-ISTM, Corso Stati Uniti 4, I-35127, Padova, Italy. roberta.seraglia@adr.pd.cnr.it
authors.forename: Roberta
authors.initials: R
authors.lastname: Seraglia
authors.forename: Giovanni
authors.initials: G
authors.lastname: Sartore
authors.forename: Raffaella
authors.initials: R
authors.lastname: Marin
authors.forename: Silvia
authors.initials: S
authors.lastname: Burlina
authors.forename: Enzo
authors.initials: E
authors.lastname: Manzato
authors.forename: Eugenio
authors.initials: E
authors.lastname: Ragazzi
authors.forename: Pietro
authors.initials: P
authors.lastname: Traldi
authors.forename: Annunziata
authors.initials: A
authors.lastname: Lapolla
mesh_terms: D000328:Adult; D016632:Apolipoprotein A-I / Q000097:blood* / Q000737:chemistry*; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D005260:Female; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood / Q000737:chemistry; D008297:Male; D008715:Methionine / Q000031:analogs & derivatives* / Q000032:analysis; D008875:Middle Aged; D018384:Oxidative Stress; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D016632:Apolipoprotein A-I; D008075:Lipoproteins, HDL; D008715:Methionine; C013111:methionine sulfoxide