title: Rapid and simple method for the determination of emodin in tartary buckwheat (Fagopyrum tataricum) by high-performance liquid chromatography coupled to a diode array detector.
abstract: A simple and rapid method for determining emodin, an active factor presented in tartary buckwheat (Fagopyrum tataricum), by high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD) has been developed. Emodin was separated from an extract of buckwheat on a Kromasil-ODS C(18) (250 mm × 4.6 mm × 5 μm) column. The separation is achieved within 15 min on the ODS column. Emodin can be quantified using an external standard method detecting at 436 nm. Good linearity is obtained with a correlation coefficient exceeding 0.9992. The limit of detection and the limit of quantification are 5.7 and 19 μg/L, respectively. This method shows good reproducibility for the quantification of the emodin with a relative standard deviation value of 4.3%. Under optimized extraction conditions, the recovery of emodin was calculated as >90%. The validated method is successfully applied to quantify the emodin in tartary buckwheat and its products.
authors.affiliation: College of Biotechnology Industries, Chengdu University, Chengdu 610106, People's Republic of China.
authors.forename: Lian-Xin
authors.initials: LX
authors.lastname: Peng
authors.forename: Jing-Bo
authors.initials: JB
authors.lastname: Wang
authors.forename: Li-Xue
authors.initials: LX
authors.lastname: Hu
authors.forename: Jiang-Lin
authors.initials: JL
authors.lastname: Zhao
authors.forename: Da-Bing
authors.initials: DB
authors.lastname: Xiang
authors.forename: Liang
authors.initials: L
authors.lastname: Zou
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004642:Emodin / Q000032:analysis*; D019612:Fagopyrum / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry
chemical_list: D010936:Plant Extracts; D004642:Emodin