title: Expression Pattern of Peroxisome Proliferator-Activated Receptors in Rat Hippocampus following Cerebral Ischemia and Reperfusion Injury.
abstract: The present study was designed to investigate the pattern of time-dependent expression of peroxisome proliferator-activated receptors (PPARα, β, and γ) after global cerebral ischemia and reperfusion (I/R) damage in the rat hippocampus. Male Sprague Dawley (SD) rats were subjected to global cerebral I/R. The rat hippocampi were isolated to detect the expression of PPARs mRNA and protein levels at 30 min-30 d after I/R by RT-PCR and Western blot analysis, respectively. The expression levels of PPARs mRNA and protein in the rat hippocampus significantly increased and peaked at 24 h for PPARα and γ (at 48 h for PPARβ) after I/R, then gradually decreased, and finally approached control levels on d 30. The present results suggest that global cerebral I/R can cause obvious increases of hippocampal PPARs mRNA and protein expression within 15 d after I/R. These findings may help to guide the experimental and clinical therapeutic use of PPARs agonists against brain injury.
authors.affiliation: The College of Pharmacy, Chongqing Medical University, Chongqing 400010, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Rong
authors.initials: R
authors.lastname: Jiang
authors.forename: Qin
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authors.lastname: He
authors.forename: Yunmei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yanli
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Ruichun
authors.initials: R
authors.lastname: Zhuang
authors.forename: Ying
authors.initials: Y
authors.lastname: Luo
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Jinyuan
authors.initials: J
authors.lastname: Wan
authors.forename: Yong
authors.initials: Y
authors.lastname: Tang
authors.forename: Huarong
authors.initials: H
authors.lastname: Yu
authors.forename: Qingsong
authors.initials: Q
authors.lastname: Jiang
authors.forename: Junqing
authors.initials: J
authors.lastname: Yang