title: Methods for the study of dopamine receptors within lipid rafts of kidney cells.
abstract: There is increasing evidence that G protein-coupled receptor (GPCR) signaling is regulated in lipid raft microdomains. GPCRs and GPCR-signaling molecules, including G proteins and protein kinases, have been reported to compartmentalize in these microdomains. Dopamine D(1)-like receptors (D(1)R and D(5)R) belong to a family of GPCRs that are important in the regulation of renal function. These receptors are not only localized and regulated in caveolae that contains caveolin-1 but are also distributed in non--caveolar lipid rafts which do not contain caveolin-1. This chapter describes detergent- and non-detergent-based methods to obtain lipid raft fractions from renal proximal tubule cells.
authors.affiliation: Department of Pediatrics, Center for Molecular Physiology Research, Children's National Medical Center, School of Medicine, George Washington University, Washington, DC, USA.
authors.forename: Peiying
authors.initials: P
authors.lastname: Yu
authors.forename: Van Anthony
authors.initials: VA
authors.lastname: Villar
authors.forename: Pedro A
authors.initials: PA
authors.lastname: Jose
mesh_terms: D002458:Cell Fractionation / Q000379:methods*; D003902:Detergents / Q000494:pharmacology; D057809:HEK293 Cells; D006801:Humans; D015151:Immunoblotting; D007668:Kidney / Q000166:cytology*; D021962:Membrane Microdomains / Q000187:drug effects / Q000378:metabolism*; D011954:Receptors, Dopamine / Q000032:analysis / Q000378:metabolism*
chemical_list: D003902:Detergents; D011954:Receptors, Dopamine