title: Phage display selection of peptides that target calcium-binding proteins.
abstract: Phage display allows to rapidly identify peptide sequences with binding affinity towards target proteins, for example, calcium-binding proteins (CBPs). Phage technology allows screening of 10(9) or more independent peptide sequences and can identify CBP binding peptides within 2 weeks. Adjusting of screening conditions allows selecting CBPs binding peptides that are either calcium-dependent or independent. Obtained peptide sequences can be used to identify CBP target proteins based on sequence homology or to quickly obtain peptide-based CBP inhibitors to modulate CBP-target interactions. The protocol described here uses a commercially available phage display library, in which random 12-mer peptides are displayed on filamentous M13 phages. The library was screened against the calcium-binding protein S100B.
authors.affiliation: Department of Pharmaceutical Sciences, North Dakota State University, Fargo, ND, USA. stefan.vetter@ndsu.edu
authors.forename: Stefan W
authors.initials: SW
authors.lastname: Vetter
mesh_terms: D017104:Bacteriophage M13; D006801:Humans; D009414:Nerve Growth Factors / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism*; D019151:Peptide Library; D011485:Protein Binding; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000378:metabolism*; D017422:Sequence Analysis, DNA
chemical_list: D009414:Nerve Growth Factors; D009842:Oligopeptides; D019151:Peptide Library; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579160:S100B protein, human