title: New method for density determination of nanoparticles using a CPS disc centrifuge™.
abstract: A model has been developed for the determination of the correct absolute size of nanoparticles using a disc centrifuge; the method does not require externally measured or literature derived particle densities. The principle of this method is the altered settling velocity of particles in fluids with different viscosities and/or densities, with the use of a linear regression analysis for the calculation of particle densities. This allows a fast particle density determination with at least two measurements using a disc centrifuge and a small subset of easily measurable parameters. Furthermore, correctness of the model is evaluated using viruses and nonbiological particles of known densities.
authors.affiliation: Novartis Vaccines and Diagnostics GmbH, Emil-von-Behring-Strasse 76, 35041 Marburg, Germany. arne-1.neumann@novartis.com
authors.forename: A
authors.initials: A
authors.lastname: Neumann
authors.forename: W
authors.initials: W
authors.lastname: Hoyer
authors.forename: M W
authors.initials: MW
authors.lastname: Wolff
authors.forename: U
authors.initials: U
authors.lastname: Reichl
authors.forename: A
authors.initials: A
authors.lastname: Pfitzner
authors.forename: B
authors.initials: B
authors.lastname: Roth
mesh_terms: D002499:Centrifugation, Density Gradient / Q000295:instrumentation / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D013499:Surface Properties; D014783:Viscosity