title: Substrate-mediated gene delivery from glycol-chitosan/hyaluronic acid polyelectrolyte multilayer films.
abstract: Substrate-mediated transfection is one of the key strategies for localized gene delivery. Layer-by-layer (LbL) polyelectrolyte deposition is a promising technique which enables controlled delivery of a number of biofactors, including nucleic acids. Here, we embed lipoplexes containing plasmid DNA within polyelectrolyte multilayers composed of glycol-chitosan (Glyc-CHI) and hyaluronic acid (HA) in order to produce a film system that enables localized, surface-based transfection. The topography and morphology of the resulting multilayers were characterized after lipoplex absorption and during subsequent film build-up via atomic force microscopy (AFM) and scanning electron microscopy (SEM), respectively. DNA embedding efficiency and release were then examined. Lipoplex-containing Glyc-CHI/HA films were found to successfully transfect NIH3T3 fibroblasts and HEK293 kidney cells in vitro, maintaining transfection levels of approximately 20% for a period of at least 7 days.
authors.affiliation: Department of Biomedical Engineering, Duff Medical Science Building, 3775 University Street, McGill University, Montreal, H3A 2B4, Canada.
authors.forename: Christina A
authors.initials: CA
authors.lastname: Holmes
authors.forename: Maryam
authors.initials: M
authors.lastname: Tabrizian
mesh_terms: D000818:Animals; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004573:Electrolytes / Q000737:chemistry*; D018014:Gene Transfer Techniques / Q000295:instrumentation*; D057809:HEK293 Cells; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D014162:Transfection
chemical_list: D004573:Electrolytes; D006820:Hyaluronic Acid; D004247:DNA