title: An efficient in vitro regeneration protocol for a natural dye yielding plant, Strobilanthes flaccidifolious Nees., from nodal explants.
abstract: Adventitious shoot buds formation from axillary buds of nodal segments of S. flaccidifolious was achieved on MS medium containing sucrose (3%, w/v), and a-naphthalene acetic acid (NAA; 3 microM) and benzyl adenine (3 microM) in combination. The nodal segments were primed on 'Growtak Sieve' for 48 h on MS medium containing sucrose (2%), polyvinyl pyrollidone (200 mgL(-1)) as antioxidant. About 80% of primed nodal segments responded positively and formed approximately 12 adventitious shoot buds per explants from explants collected during October-November months of every year. The shoot buds converted into plantlets on MS medium containing sucrose (3%) and kinetin (3 microM) where approximately 7 micro shoots developed per subculture after 8 weeks of culture. The regenerated micro shoots induced average 14 roots/plant on medium containing NAA (3 microM). The regenerates were hardened for 6-7 weeks on medium with 1/2MS salt solution and sucrose (2%) under normal laboratory condition before transferring to potting mix. About 70% transplants survived after two months of transfer.
authors.affiliation: Department of Botany, Nagaland University, Lumami 798 627, India. debchitta@rediffmail.com
authors.forename: Chitta Ranjan
authors.initials: CR
authors.lastname: Deb
authors.forename: T
authors.initials: T
authors.lastname: Arenmongla
mesh_terms: D027901:Acanthaceae / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology; D046508:Culture Techniques; D007194:India; D010860:Pigments, Biological / Q000302:isolation & purification* / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology; D018521:Plant Physiological Phenomena; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D012038:Regeneration; D012621:Seasons
chemical_list: D010860:Pigments, Biological; D010937:Plant Growth Regulators