title: Comparative study of the behavior of p53 immunoexpression in smoking associated lesions: Reinke's edema and laryngeal carcinoma.
abstract: OBJECTIVE
  To assess the behavior of the immunoexpression of protein p53 in Reinke's edema and laryngeal squamous cell carcinoma.
  
  
  METHODS
  retrospective.
  
  
  METHODS
  we recovered the histological paraffin blocks of patients who were subjected to Reinke's edema and laryngeal squamous cell carcinoma surgery in 2000-2011. The paraffin blocks were cut into 3-µm sections; the specimens were prepared in silanized slides (one slide for each paraffin block) and subjected to immunohistochemical reaction according to the Avidin Biotin Peroxidase method. Monoclonal primary anti-p53 antibodies were used at 1:50 dilution. Slides were examined under a light microscope at different magnitudes and results were interpreted based on the degree of brown staining in the nuclei of epithelial cells and in the extent of the fragment by using a semi-quantitative score from 0 to 3.
  
  
  RESULTS
  67 slides of Reinke's edema and 60 slides of laryngeal squamous cell carcinoma were included. Scores 2 and 3 for staining of the nuclei of epithelial cells were recorded for 46 slides of Reinke's edema (68.65%) and for 57 slides of laryngeal squamous cell carcinoma (95%). As to the extent of the fragment, scores 2 and 3 were recorded for 74% slides of Reinke's edema and for 95% slides of carcinomas.
  
  
  CONCLUSIONS
  the positive immunoexpression for protein p53, positive in 95% carcinomas and 74% Reinke's edemas, makes us aware of the possible preneoplastic condition of the latter lesion. Further studies are needed to identify and reveal the genetic changes that lead to these results.
authors.affiliation: Botucatu Medical School, UNESP, Sao Paulo, Brazil. lesmoz@uol.com.br
authors.forename: Luis Eduardo Silva
authors.initials: LE
authors.lastname: Móz
authors.forename: Maria Aparecida Custódio
authors.initials: MA
authors.lastname: Domingues
authors.forename: Emanuel Celice
authors.initials: EC
authors.lastname: Castilho
authors.forename: Anete
authors.initials: A
authors.lastname: Branco
authors.forename: Regina Helena Garcia
authors.initials: RH
authors.lastname: Martins
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007819:Laryngeal Edema / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D007820:Laryngeal Mucosa / Q000378:metabolism / Q000473:pathology; D007822:Laryngeal Neoplasms / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011230:Precancerous Conditions / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D012189:Retrospective Studies; D012907:Smoking / Q000009:adverse effects / Q000378:metabolism* / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*
chemical_list: C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53