title: Butanol production from acid hydrolyzed corn fiber with Clostridium beijerinckii mutant.
abstract: Sulfuric acid treated corn fiber hydrolysate (SACFH) inhibited cell growth and the production of butanol (4.7±0.2 g/L) by Clostridium beijerinckii IB4 in P2 medium. Optimal medium components were determined using fractional factorial design. NH4HCO3, FeSO4·7H2O and CaCO3 were demonstrated to be significant components in the production of butanol. The Box-Behnken design and a corresponding quadratic model were used to predict medium components (NH4HCO3 1.96 g/L, FeSO4·7H2O 0.26 g/L and CaCO3 3.15 g/L) and butanol yield (9.5 g/L). The confirmation experiment, under the predicted optimal conditions, yielded a butanol level of 9.5±0.1g/L. This study indicates that the Box-Behnken design is an effective approach for screening the optimal medium components required for the production of butanol. It also demonstrates that SACFH, which has high levels of inhibitors such as furan and phenolic compounds, may be used as a renewable carbon source in the production of biofuels.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 211816, People's Republic of China.
authors.forename: Teng-fei
authors.initials: TF
authors.lastname: Du
authors.forename: Ai-yong
authors.initials: AY
authors.lastname: He
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Jia-nan
authors.initials: JN
authors.lastname: Chen
authors.forename: Xiang-ping
authors.initials: XP
authors.lastname: Kong
authors.forename: Jun-li
authors.initials: JL
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Ping-kai
authors.initials: PK
authors.lastname: Ouyang
mesh_terms: D000704:Analysis of Variance; D000440:Butanols / Q000378:metabolism*; D002244:Carbon / Q000494:pharmacology; D046970:Clostridium beijerinckii / Q000187:drug effects / Q000235:genetics*; D003470:Culture Media / Q000494:pharmacology; D006868:Hydrolysis / Q000187:drug effects; D009154:Mutation / Q000235:genetics*; D015203:Reproducibility of Results; D013464:Sulfuric Acids / Q000494:pharmacology*; D003313:Zea mays / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000440:Butanols; D003470:Culture Media; D013464:Sulfuric Acids; D002244:Carbon; C033158:sulfuric acid