title: Detection of Aichi virus with antibody targeting of conserved viral protein 1 epitope.
abstract: Aichi virus (AiV) is an emerging single-stranded, positive-sense, non-enveloped RNA virus in the Picornaviridae that causes acute gastroenteritis in humans. The first case of AiV infection in Taiwan was diagnosed in a human neonate with enterovirus-associated symptoms; the virus was successfully isolated and propagated. To establish a method to detect AiV, we analyzed the antigen epitope and generated a polyclonal antibody against AiV viral protein 1 (VP1). This peptide-purified anti-AiV VP1 antibody showed high specificity against AiV VP1 without cross-reaction to nine other tested strains of Picornaviruses. The anti-AiV VP1 antibody was used in immunofluorescence analysis, immunoblotting, and enzyme-linked immunosorbent assay to elucidate the cell tropism and replication kinetics of AiV. Use of the anti-AiV VP1 antibody also revealed AiV infection restriction with interferon type I and polyI/C antiviral treatment. The AiV infection and detection system may provide an in vitro platform for AiV virology study.
authors.affiliation: Department of Infectious Diseases, Kaohsiung Veterans General Hospital, 386, Ta-chung 1st road, Kaohsiung, 813, Taiwan.
authors.forename: Yao-Shen
authors.initials: YS
authors.lastname: Chen
authors.forename: Bao-Chen
authors.initials: BC
authors.lastname: Chen
authors.forename: You-Sheng
authors.initials: YS
authors.lastname: Lin
authors.forename: Jenn-Tzong
authors.initials: JT
authors.lastname: Chang
authors.forename: Tsi-Shu
authors.initials: TS
authors.lastname: Huang
authors.forename: Jih-Jung
authors.initials: JJ
authors.lastname: Chen
authors.forename: Tsung-Hsien
authors.initials: TH
authors.lastname: Chang
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000276:immunology; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D052699:Kobuvirus / Q000302:isolation & purification*; D010850:Picornaviridae Infections / Q000821:virology*; D013624:Taiwan; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology; D014773:Virology / Q000379:methods*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D014764:Viral Proteins