title: Microsphere-embedded cryogel for selective and efficient depletion of immunoglobulin G from human serum.
abstract: In this study a novel composite cryogel column was synthesized for affinity depletion of immunoglobulin G (IgG) from human serum. The microsphere-embedding technique combines the large surface area of microspheres with excellent properties of cryogels. The poly(hydroxyethyl methacrylate- N-methacryloyl-L-histidine methyl ester) microsphere (2 μm size)- embedded composite cryogel (MECC) column was synthesized and characterized by various methods. The specific surface area of the composite cryogel was found as 92 m(2)/g by using BET measurement. Maximum HIgG adsorption on MECC column was found as 50.5 mg/g. MECC column can be reused many times with no apparent decrease in HIgG adsorption capacity.
authors.affiliation: Chemistry Department, Biochemistry Division, Hacettepe University , Ankara , Turkey.
authors.forename: Gözde
authors.initials: G
authors.lastname: Baydemir
authors.forename: Mehmet
authors.initials: M
authors.lastname: Odabaşı
mesh_terms: D000327:Adsorption; D059985:Cryogels / Q000138:chemical synthesis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000378:metabolism*; D007163:Immunosorbent Techniques; D008689:Methacrylates / Q000138:chemical synthesis*; D008863:Microspheres; D044967:Serum / Q000378:metabolism*
chemical_list: D059985:Cryogels; D007074:Immunoglobulin G; D008689:Methacrylates; C005044:hydroxyethyl methacrylate