title: Evaluation of an electrochemical method for the analysis of enzymatic inhibition reactions.
abstract: An approximate equation for bioelectrocatalitic current was applied to an inhibition reaction analysis of bilirubin oxidase by anion (Cl(-), SCN(-), and F(-)) in order to assess the possibility of the electrochemical method for the analysis of enzymatic inhibition reactions.  The approximate equation can be transformed into the Michaelis-Menten form, so that the bioelectrocatalitic current can be analyzed by the usual graphical manner, that is, Lineweaver-Burk, Hanes-Woolf, Dixon and Cornish-Bowden plots, if the rate of inhibition reaction is described as a simple Michaelis-Menten form.  From the electrochemical assay, it was found that the inhibitor of Cl(-) and SCN(-) anions exhibit non-competitive inhibition while that of F(-) exhibits competitive inhibition, and their inhibition constants were 220, 45, and 22 mM, respectively.  The results were essentially similar to those obtained from the conventional spectrophotometric assay.
authors.affiliation: Faculty of Biotechnology, Fukui Prefectural University, Eiheiji, Fukui, Japan. kuematsu@fpu.ac.jp
authors.forename: Kohei
authors.initials: K
authors.lastname: Uematsu
authors.forename: Hajime
authors.initials: H
authors.lastname: Katano
mesh_terms: D001667:Binding, Competitive; D055664:Electrochemical Techniques / Q000379:methods*; D004791:Enzyme Inhibitors / Q000737:chemistry*; D005295:Ferrocyanides / Q000737:chemistry; D007700:Kinetics; D008956:Models, Chemical; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000737:chemistry; D011189:Potassium Chloride / Q000737:chemistry; D012969:Sodium Fluoride / Q000737:chemistry; D013053:Spectrophotometry; D013861:Thiocyanates / Q000737:chemistry
chemical_list: D004791:Enzyme Inhibitors; D005295:Ferrocyanides; D013861:Thiocyanates; C024553:sodium thiocyanate; D011189:Potassium Chloride; D012969:Sodium Fluoride; D044925:Oxidoreductases Acting on CH-CH Group Donors; C042401:bilirubin oxidase