title: Semi-automatic determination of cell surface areas used in systems biology.
abstract: Quantitative biology requires high precision measurement of cellular parameters such as surface areas or volumes. Here, we have developed an integrated approach in which the data from 3D confocal microscopy and 2D high-resolution transmission electron microscopy were combined. The volumes and diameters of the cells within one population were automatically measured from the confocal data sets. The perimeter of the cell slices was measured in the TEM images using a semi-automated segmentation into background, cytoplasm and nucleus. These data in conjunction with approaches from stereology allowed for an unbiased estimate of surface areas with high accuracy. We have determined the volumes and surface areas of the cells and nuclei of six different immune cell types. In mast cells for example, the resulting cell surface was 3.5 times larger than the theoretical surface assuming the cell was a sphere with the same volume. Thus, our accurate data can now serve as inputs in modeling approaches in systems immunology.
authors.affiliation: BIOSS Centre for Biological Signaling Studies, University of Freiburg, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Morath
authors.forename: Margret
authors.initials: M
authors.lastname: Keuper
authors.forename: Marta
authors.initials: M
authors.lastname: Rodriguez-Franco
authors.forename: Sumit
authors.initials: S
authors.lastname: Deswal
authors.forename: Gina
authors.initials: G
authors.lastname: Fiala
authors.forename: Britta
authors.initials: B
authors.lastname: Blumenthal
authors.forename: Daniel
authors.initials: D
authors.lastname: Kaschek
authors.forename: Jens
authors.initials: J
authors.lastname: Timmer
authors.forename: Gunther
authors.initials: G
authors.lastname: Neuhaus
authors.forename: Stephan
authors.initials: S
authors.lastname: Ehl
authors.forename: Olaf
authors.initials: O
authors.lastname: Ronneberger
authors.forename: Wolfgang Werner A
authors.initials: WW
authors.lastname: Schamel
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000648:ultrastructure*; D002460:Cell Line; D048429:Cell Size; D005434:Flow Cytometry; D006801:Humans; D007107:Immune System / Q000166:cytology*; D019169:Jurkat Cells / Q000648:ultrastructure*; D008407:Mast Cells / Q000648:ultrastructure*; D051379:Mice; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D018448:Models, Immunological; D049490:Systems Biology / Q000379:methods*