title: Development and interlaboratory validation of quantitative polymerase chain reaction method for screening analysis of genetically modified soybeans.
abstract: A novel real-time polymerase chain reaction (PCR)-based quantitative screening method was developed for three genetically modified soybeans: RRS, A2704-12, and MON89788. The 35S promoter (P35S) of cauliflower mosaic virus is introduced into RRS and A2704-12 but not MON89788. We then designed a screening method comprised of the combination of the quantification of P35S and the event-specific quantification of MON89788. The conversion factor (Cf) required to convert the amount of a genetically modified organism (GMO) from a copy number ratio to a weight ratio was determined experimentally. The trueness and precision were evaluated as the bias and reproducibility of relative standard deviation (RSDR), respectively. The determined RSDR values for the method were less than 25% for both targets. We consider that the developed method would be suitable for the simple detection and approximate quantification of GMO.
authors.affiliation: National Food Research Institute, National Agriculture and Food Research Organization, 2–1–12 Kannondai, Tsukuba, Ibaraki 305–8642, Japan.
authors.forename: Reona
authors.initials: R
authors.lastname: Takabatake
authors.forename: Mari
authors.initials: M
authors.lastname: Onishi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Koiwa
authors.forename: Satoshi
authors.initials: S
authors.lastname: Futo
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Minegishi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Akiyama
authors.forename: Reiko
authors.initials: R
authors.lastname: Teshima
authors.forename: Takeyo
authors.initials: T
authors.lastname: Kurashima
authors.forename: Junichi
authors.initials: J
authors.lastname: Mano
authors.forename: Satoshi
authors.initials: S
authors.lastname: Furui
authors.forename: Kazumi
authors.initials: K
authors.lastname: Kitta
mesh_terms: D018744:DNA, Plant / Q000032:analysis*; D030841:Food, Genetically Modified; D030821:Plants, Genetically Modified; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D013025:Glycine max / Q000235:genetics*
chemical_list: D018744:DNA, Plant