title: Loss of heterozygosity at 11p13 and 11p15 in Wilms tumor: a study of 22 cases from India.
abstract: OBJECTIVE
  11p13 and 11p15 loss of heterozygosity (LOH) in Wilms tumor (WT), the commonest molecular pathogenetic event in WT, shows variation in different parts of the world. The present study looked for the presence of 11p13 and 11p15 LOH as well as nephrogenic rests in WT occurring in India.
  
  
  METHODS
  Twenty-two cases of WT were subjected to thorough pathological examination for presence of nephrogenic rests. Fresh frozen tissue was evaluated for LOH at 11p13 and 11p15, using PCR for microsatellite markers.
  
  
  RESULTS
  Among twenty-two consecutive cases of WT, 20 were unilateral and 2 were bilateral. 6/22 showed LOH at 11p13 (27.7 %) and 1/22 showed LOH at 11p15 (4.54 %). 2/22 cases showed presence of nephrogenic rests. One of the cases with LOH at 11p13 had intralobar nephrogenic rest in the adjacent kidney. One specimen had perilobar nephrogenic rest in the adjacent kidney but did not show LOH for either 11p13 or 11p15 in the tumor.
  
  
  CONCLUSIONS
  LOH at 11p13 is seen in 27.27 % of WT in India, which is similar to reports in the English language literature. LOH at 11p15 was seen in 4.54 % of WT, which is lower than that reported from Western subjects.
authors.affiliation: Department of Pathology, All India Institute of Medical Sciences, New Delhi 110029, India.
authors.forename: Elanthenral
authors.initials: E
authors.lastname: Sigamani
authors.forename: Mohammad Nahidul
authors.initials: MN
authors.lastname: Wari
authors.forename: Venkateswaran K
authors.initials: VK
authors.lastname: Iyer
authors.forename: Sandeep
authors.initials: S
authors.lastname: Agarwala
authors.forename: Arundhati
authors.initials: A
authors.lastname: Sharma
authors.forename: Sameer
authors.initials: S
authors.lastname: Bakhshi
authors.forename: Amit
authors.initials: A
authors.lastname: Dinda
mesh_terms: D002648:Child; D002675:Child, Preschool; D002880:Chromosomes, Human, Pair 11; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D006801:Humans; D007223:Infant; D007680:Kidney Neoplasms / Q000235:genetics*; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D009396:Wilms Tumor / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm