title: Sandwich-type electrochemical biosensor for glycoproteins detection based on dual-amplification of boronic acid-gold nanoparticles and dopamine-gold nanoparticles.
abstract: Glycoproteins play important roles in a wide variety of biological processes. The change in the concentration levels has been associated with many cancers, as well as other diseases. Thus, rapid, sensitive and selective determination of glycoproteins is much preferred. In this work, we reported a sandwich-type electrochemical biosensor based on dual-amplification of 4-mercaptophenylboronic acid (MBA)-capped gold nanoparticles (MBA-AuNPs) and dopamine (DA)-capped AuNPs (DA-AuNPs). Biological recognition elements such as synthetic receptor and aptamer immobilized onto gold electrodes were used to capture glycoproteins. The captured glycoproteins were then derivatized with MBA-AuNPs through the formation of tight covalent bonds between the boronic acids of MBA-AuNPs and diols of glycoproteins. Electroactive DA-AuNPs were attached by the anchored MBA-AuNPs via the interaction of boronic acids with DA tags, which facilities the amplified voltammetric detection of glycoproteins. With avidin and prostate specific antigen (PSA) as model analytes, we demonstrated the feasibility and sensitivity of the proposed method. The results indicated that sub-picomolar avidin/PSA can be readily measured. We believe that this strategy will be valuable for the electrochemical detection of other glycoproteins.
authors.affiliation: College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, People's Republic of China. xianing82414@csu.edu.cn
authors.forename: Ning
authors.initials: N
authors.lastname: Xia
authors.forename: Dehua
authors.initials: D
authors.lastname: Deng
authors.forename: Liping
authors.initials: L
authors.lastname: Zhang
authors.forename: Baiqing
authors.initials: B
authors.lastname: Yuan
authors.forename: Min
authors.initials: M
authors.lastname: Jing
authors.forename: Jimin
authors.initials: J
authors.lastname: Du
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D001897:Boronic Acids / Q000737:chemistry*; D003217:Conductometry / Q000295:instrumentation*; D004298:Dopamine / Q000737:chemistry*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006023:Glycoproteins / Q000032:analysis* / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D001897:Boronic Acids; D006023:Glycoproteins; D006046:Gold; D004298:Dopamine