title: Identification of genes differentially expressed in clams Ruditapes philippinarum in response to endosulfan after different exposure time.
abstract: This study reports molecular biomarkers potentially associated with resistance or sensitivity to the impact of endosulfan in clams, Ruditapes philippinarum. Genomic analysis was made applying suppression subtractive hybridization (SSH) to identify genes up- and down-regulated in calms exposed to 0.5 μg/L endosulfan for 1d and 10d. Results obtained from the SSH library revealed 360 different sequences regulated by endosulfan, which correspond to nine major physiological functions. To validate the expression profiles from SSH, 13 genes regulated by endosulfan were measured by quantitative real-time PCR. This study provides information for the characterization of potential molecular biomarker that may be used in future environmental monitoring and to investigate the mechanisms of stress to endosulfan in clams/marine invertebrates.
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Tao
authors.forename: Luqing
authors.initials: L
authors.lastname: Pan
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Na
authors.initials: N
authors.lastname: Liu
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D004726:Endosulfan / Q000633:toxicity*; D004784:Environmental Monitoring; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D015723:Gene Library; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D004726:Endosulfan