title: [Screening of Clostridium strains through ribosome engineering for improved butanol production].
abstract: We used ribosome engineering technology, with which antibiotic-resistant strains are resulted from mutations on microbial ribosome, to screen a high butanol-producing Clostridium strain. A novel mutant strain S3 with high butanol production and tolerance was obtained from the original Clostridium acetobutylicum L7 with the presence of mutagen of streptomycin. Butanol of 12.48 g/L and ethanol of 1.70 g/L were achieved in S3, 11.2% and 50%, respectively higher than the parent strain. The conversion rate of glucose to butanol increased from 0.19 to 0.22, and fermentation time was 9 h shorter. This caused an increase in butanol productivity by 30.5%, reaching 0.24 g/(Lh). The mutant butanol tolerance was increased from 12 g/L to 14 g/L, the viscosity of fermentation broth was dramatically decreased to 4 mPa/s, 60% lower than the parent strain. In addition, the genetic stability of mutant strain S3 was also favorable. These results demonstrate that ribosome engineering technology may be a promising process for developing high butanol-producing strains.
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, Liaoning, China. ljchen@dlut.edu.cn
authors.forename: Lijie
authors.initials: L
authors.lastname: Chen
authors.forename: Guanglai
authors.initials: G
authors.lastname: Shang
authors.forename: Wenjie
authors.initials: W
authors.lastname: Yuan
authors.forename: Youduo
authors.initials: Y
authors.lastname: Wu
authors.forename: Fengwu
authors.initials: F
authors.lastname: Bai
mesh_terms: D000440:Butanols / Q000378:metabolism*; D046969:Clostridium acetobutylicum / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D005285:Fermentation; D005818:Genetic Engineering; D009154:Mutation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012270:Ribosomes / Q000235:genetics*; D013307:Streptomycin / Q000494:pharmacology
chemical_list: D000440:Butanols; D011994:Recombinant Proteins; D013307:Streptomycin