title: Electrochemically modified carbon and chromium surfaces for AFM imaging of double-strand DNA interaction with transposase protein.
abstract: Carbon and chromium surfaces were modified by electrochemical reduction of a diazonium salt formed in situ from the sulfanilic acid. The organic layer formed was activated by phosphorus pentachloride (PCl(5)) to form a benzene sulfonil chloride (Ar-SO(2)Cl). An electrochemical study of the blocking effect and the activity of this surface was carried out on a carbon electrode. The chromium surface study was completed by X-ray photoelectron spectroscopy and atomic force microscopy to characterize the formation of a compact monolayer (0.8 nm height and roughness 0.2-0.3 nm). The compactness and the activity of this organic monolayer allowed us to affix a length dsDNA with the aim of analyzing the formation of a complex between dsDNA and a protein. The interaction of a transposase protein with its target dsDNA was investigated. The direct imaging of the nucleoproteic complex considered herein gives new insights in the comprehension of transposase-DNA interaction in agreement with biochemical data.
authors.affiliation: Département Méthodologie et Synthèse, Équipe Méthodologie en Synthèse des Polymères, Institut des Molécules et Matériaux du Mans, UMR CNRS, Université du Maine, Faculté des Sciences, Le Mans, France.
authors.forename: Charles
authors.initials: C
authors.lastname: Esnault
authors.forename: Benoît
authors.initials: B
authors.lastname: Chénais
authors.forename: Nathalie
authors.initials: N
authors.lastname: Casse
authors.forename: Nicolas
authors.initials: N
authors.lastname: Delorme
authors.forename: Guy
authors.initials: G
authors.lastname: Louarn
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Pilard
mesh_terms: D002857:Chromium / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D055664:Electrochemical Techniques; D004566:Electrodes; D018625:Microscopy, Atomic Force; D013499:Surface Properties; D019895:Transposases / Q000737:chemistry* / Q000378:metabolism
chemical_list: D002857:Chromium; D004247:DNA; D019895:Transposases