title: Physicochemical and biochemical profiling of diphenyl diselenide.
abstract: The objective of the present study was to evaluate the physicochemical and biochemical profiling of diphenyl diselenide (PhSe)₂, a selenoorganic compound with biological activity. Experimental protocols were established for chemical stability in isotonic phosphate buffer (PBS) pH 7.4 and in simulated gastric and intestinal fluids, biological stability (bovine serum albumin (BSA) and plasma), solubility in PBS pH 7.4, distribution coefficient (Log D) in octanol/PBS, and determination of free (PhSe)₂ concentrations in BSA and plasma by using liquid chromatography with ultraviolet detection and tandem mass spectrometry. (PhSe)₂ was found to be chemically stable and not susceptible to degradation in plasma. The aqueous solubility was 0.98 ± 0.072 μM and the Log D in octanol/PBS system was found to be 3.13. The percentage of unbound fractions of (PhSe)₂ obtained by equilibrium dialysis from BSA and plasma incubated with 100 μM (PhSe)₂ were 0.69 ± 0.12 and 0.44 ± 0.09 %, respectively. The findings indicated that (PhSe)₂ presents chemical and biological stability. Though, the compound showed low aqueous solubility, high Log D value and high binding to plasmatic protein. These data contribute to the knowledge of the toxicokinetic properties of (PhSe)₂ and further explain its low bioavailability in experimental models.
authors.affiliation: Universidade Federal do Pampa-UNIPAMPA, Campus Itaqui, CEP 97.650-000, Itaqui, RS, Brazil. marinaprigol@gmail.com
authors.forename: Marina
authors.initials: M
authors.lastname: Prigol
authors.forename: Cristina W
authors.initials: CW
authors.lastname: Nogueira
authors.forename: Gilson
authors.initials: G
authors.lastname: Zeni
authors.forename: Maria Rosário
authors.initials: MR
authors.lastname: Bronze
authors.forename: Luís
authors.initials: L
authors.lastname: Constantino
mesh_terms: D001555:Benzene Derivatives / Q000737:chemistry*; D016566:Organoselenium Compounds / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012995:Solubility
chemical_list: D001555:Benzene Derivatives; D016566:Organoselenium Compounds; C061132:diphenyldiselenide; D012710:Serum Albumin, Bovine