title: Aberrant expression of miR-199a-3p and its clinical significance in colorectal cancers.
abstract: Aberrant miR-199a-3p expression has been reported in several cancers. However, the clinical significance of miR-199a-3p in human colorectal cancer has not been addressed. In this study, we detected miR-199a-3p expression in 92 colorectal cancer cases to evaluate its clinicopathologic characteristics in colorectal cancer. We showed that miR-199a-3p expression was significantly upregulated in cancer tissues than NATs. Clinicopathologic analysis revealed that high miR-199a-3p expression contributed to more advanced lymphatic invasion, lymph node metastasis, liver metastases and late TNM stage in colorectal cancer. Kaplan-Meier analysis showed that high expression of miR-199a-3p could lead to a significantly shorter overall survival rate. Cox's proportional hazards model also indicated that the high expression of miR-199a-3p could serve as an independent and significant prognostic factor for survival. We transfected miR-199a-3p inhibitor into SW480 cells and observed that miR-199a-3p inhibitor could markedly inhibit the cell proliferation. Flow cytometry analysis also found that miR-199a-3p inhibitor could cause G0/G1 arrest, decreased percentage of S and G2/M phase and induce more cell apoptosis in SW480 cells. These results suggested that miR-199a-3p may serve as an efficient biomarker for diagnosis and novel prognostic indicator in colorectal cancer.
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Daiwei
authors.initials: D
authors.lastname: Wan
authors.forename: Songbing
authors.initials: S
authors.lastname: He
authors.forename: Binhui
authors.initials: B
authors.lastname: Xie
authors.forename: Guohui
authors.initials: G
authors.lastname: Xu
authors.forename: Wen
authors.initials: W
authors.lastname: Gu
authors.forename: Chenglong
authors.initials: C
authors.lastname: Shen
authors.forename: You
authors.initials: Y
authors.lastname: Hu
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Wang
authors.forename: Qiaoming
authors.initials: Q
authors.lastname: Zhi
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000032:analysis / Q000096:biosynthesis*; D008875:Middle Aged; D009367:Neoplasm Staging; D016016:Proportional Hazards Models; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; C531408:mirn199 microRNA, human