title: Synthesis of 5-hydroxymethyl-, 5-formyl-, and 5-carboxycytidine-triphosphates and their incorporation into oligonucleotides by polymerase chain reaction.
abstract: The synthesis of the triphosphates of 5-hydroxymethyl-, 5-formyl-, and 5-carboxycytidine and the incorporation of these building blocks into long DNA fragments using the polymerase chain reaction (PCR) are reported. In this way DNA fragments containing multiple hmC, fC, and caC nucleobases are readily accessible.
authors.affiliation: Center for Integrated Protein Science at the Department of Chemistry, Ludwig-Maximilians Universität München, Butenandtstr. 5-13, 81377, Munich, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Steigenberger
authors.forename: Stefan
authors.initials: S
authors.lastname: Schiesser
authors.forename: Benjamin
authors.initials: B
authors.lastname: Hackner
authors.forename: Caterina
authors.initials: C
authors.lastname: Brandmayr
authors.forename: Silvia K
authors.initials: SK
authors.lastname: Laube
authors.forename: Jessica
authors.initials: J
authors.lastname: Steinbacher
authors.forename: Toni
authors.initials: T
authors.lastname: Pfaffeneder
authors.forename: Thomas
authors.initials: T
authors.lastname: Carell
mesh_terms: D004247:DNA / Q000737:chemistry*; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011122:Polyphosphates / Q000737:chemistry
chemical_list: D009841:Oligonucleotides; D011122:Polyphosphates; D004247:DNA; C005692:triphosphoric acid