title: [Differentiation of rat bone marrow mesenchymal stem cells into adipocytes and blockage of the differentiation].
abstract: OBJECTIVE
  To explore the pathogenesis of tumors by blocking the normal differentiation process of stem cells.
  
  
  METHODS
  Bone marrow mesenchymal stem cells (BMSCs) from rats were isolated, cultured and purified by whole bone marrow adherence method. The rat BMSCs were induced to differentiate into adipocytes with dexamethasone, insulin and indomethacin. Blockage of the differentiation process was induced by 3-methylcholanthrene (3-MC).
  
  
  RESULTS
  The differentiation experiment showed that at 30 days after the induction, oil red O staining-positive cells occurred with increased intracytolasmic lipid droplets, characteristic for adipocytes. The differentiation blockage experiment showed that at 30 days after induction, the deposits of oil red O staining-cytoplasmic lipid droplets was significantly reduced, indicating that the blocked cells were adipocytes, but not fully differentiated. Morphological identification showed that cell contact inhibition disappeared, abnormal cell nuclei, increased number of micronucleus aberration and karyotype abnormalities, indicating that malignant transformation of the stem cells occurred after the differentiation blockage.
  
  
  CONCLUSIONS
  The results of this study show a blockage of the differentiation of that stem cells at the intermediate phase, and a tendency of malignant transformation of the stem cells. The results of our study provide new evidence that cancer stem cells may be originated by suppression of stem cell differentiation.
authors.affiliation: Gansu Academy of Medical Sciences, Gansu Tumor Hospital, Lanzhou, China.
authors.forename: Xue-zhong
authors.initials: XZ
authors.lastname: Chen
authors.forename: Jie-yu
authors.initials: JY
authors.lastname: Zhang
authors.forename: Jia-yu
authors.initials: JY
authors.lastname: Zeng
authors.forename: Rong
authors.initials: R
authors.lastname: Yang
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Wei-bo
authors.initials: WB
authors.lastname: Zhang
authors.forename: Yu-xian
authors.initials: YX
authors.lastname: Wang
authors.forename: Ai-hong
authors.initials: AH
authors.lastname: Mao
authors.forename: Wen-ru
authors.initials: WR
authors.lastname: Tang
authors.forename: Shi-qi
authors.initials: SQ
authors.lastname: Liao
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D004338:Drug Combinations; D005260:Female; D007213:Indomethacin / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D008748:Methylcholanthrene / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004338:Drug Combinations; D007328:Insulin; D008748:Methylcholanthrene; D003907:Dexamethasone; D007213:Indomethacin