title: Confocal microscopy study of pertussis toxin and toxoids on CHO-cells.
abstract: Pertussis toxin in its detoxified form is a major component of all current acellular pertussis vaccines. Here we report the membrane translocation and internalization activities of pertussis toxin and various pertussis toxoids using Chinese hamster ovary cells and confocal microscopy based on indirect immunofluorescence labeling. Chemically detoxified pertussis toxoids were able to translocate/internalize into cells at the concentration about 1,000 times higher than the native toxin. Pertussis toxoids detoxified with different procedures (glutaraldehyde, glutaraldehyde plus formaldehyde, hydrogen peroxide or genetic mutation) showed differences in fluorescence intensity under the same condition, indicating toxoids from different detoxification methods may have different translocation/internalization activities on cells.
authors.affiliation: National Institutes for Food and Drug Control; Beijing, China; Graduate School of Peking Union Medical College; Beijing, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Tan
authors.forename: Roland A
authors.initials: RA
authors.lastname: Fleck
authors.forename: Catpagavalli
authors.initials: C
authors.lastname: Asokanathan
authors.forename: Chun-Ting
authors.initials: CT
authors.lastname: Yuen
authors.forename: Dorothy
authors.initials: D
authors.lastname: Xing
authors.forename: Shumin
authors.initials: S
authors.lastname: Zhang
authors.forename: Junzhi
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D016466:CHO Cells; D003412:Cricetulus; D018613:Microscopy, Confocal; D037342:Pertussis Toxin / Q000378:metabolism*; D021381:Protein Transport; D014121:Toxoids / Q000378:metabolism*
chemical_list: D014121:Toxoids; D037342:Pertussis Toxin