title: [Expression of cytokines in dentogingival sulcus in patients after dental implantation and during development of peri-implantitis].
abstract: OBJECTIVE
  Determination of cytokine content in various areas of dentition in patients with peri-implantitis associated with parodontopathogenic bacteria species of I and II order.
  
  
  METHODS
  32 patients with complications that developed in 3 months to 14 years after installation of intraosteal dental implants were examined. Content of cytokines in various areas of dentition was determined by using solid phase enzyme immunoassay in patients with developed peri-implantitis associated with parodontopathogenic bacteria species of I and II order. Multiplex polymerase chain reaction was used for determination of parodontopathogenic bacteria marker DNA.
  
  
  RESULTS
  Marker DNA of I order parodontopathogenic bacteria - Aggregatibacter (Actinobacillus) actinomycetemcomitans, Tannerella forsythia (Bacteroides forsythus), Porphyromonas gingivalis in peri-implantation tissues during implant rejection was detected with 34.4 - 75% frequency while II order (Prevotella intermedia, Treponema denticola, Parvimonas micros (Peptostreptococcus micros), Fusobacterium nucleatum/periodonticum etc.) - with significantly lower frequency. Total concentration of IL- 1beta, IL-4, IL-6, IL-8, TNFalpha, IL- 17A and INFgamma in contents of pathological pocket in the area of implants and the levels of each of them were significantly higher than in the contents of parodontal pockets, areas with stable implants and gingival fluid from areas with healthy teeth. Total interleukin content in the contents of pathological pockets in the area of rejected implants was significantly higher than in other studied areas. In the exudate of parodontal pockets of the preserved teeth it was 2.4 times lower, in the area of stable implants - 4.6 times lower, and in the areas with healthy teeth - 4.8 times lower than with rejected implants (p<0.05).
  
  
  CONCLUSIONS
  The results of the study conducted allow to make a conclusion regard- ing reasonability of monitoring during dental implantation of parodontopathogenic microorganism strains and local cytokine response of the host organism with the objective of rational prophylaxis and therapy of inflammatory complications.
authors.forename: V N
authors.initials: VN
authors.lastname: Tsarev
authors.forename: E N
authors.initials: EN
authors.lastname: Nikolaeva
authors.forename: E V
authors.initials: EV
authors.lastname: Ippolitov
authors.forename: T V
authors.initials: TV
authors.lastname: Tsareva
mesh_terms: D000328:Adult; D000368:Aged; D016207:Cytokines / Q000096:biosynthesis* / Q000276:immunology; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D003759:Dental Implantation, Endosseous, Endodontic; D015921:Dental Implants / Q000382:microbiology*; D005260:Female; D005881:Gingiva / Q000276:immunology / Q000382:microbiology; D006090:Gram-Negative Bacteria / Q000276:immunology* / Q000472:pathogenicity; D016905:Gram-Negative Bacterial Infections / Q000150:complications / Q000276:immunology* / Q000382:microbiology; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D057873:Peri-Implantitis / Q000150:complications / Q000276:immunology* / Q000382:microbiology
chemical_list: D016207:Cytokines; D004269:DNA, Bacterial; D015921:Dental Implants