title: Fusion of bolaamphiphile micelles: a method to prepare stable supported biomimetic membranes.
abstract: Supported biomimetic membranes (SBMs) on solid substrates have been commonly prepared from vesicle-forming double-tail lipids, such as zwitterionic phospholipids, using the method of vesicle fusion. Here we report on the preparation of SBMs on silica surfaces via a similar process of "micelle fusion" from a cationic single-tail bolaamphiphile GLH-20 that forms spherical and elongated thread-like micelles in solution. We demonstrate that, in contrast to zwitterionic phospholipids, GLH-20 self-assembles into a stable contiguous SBM at both low and high ionic strengths. The cationic charge of GLH-20 promotes the formation of a stable SBM through enhanced double-layer interactions with the negatively charged silica surface. It is also shown that spinach aquaporin PM-28 was successfully incorporated within bolaamphiphile SBM in a manner similar to SBMs prepared by vesicle/proteoliposome fusion; thereby the inherent curvature of the micelle surface does not inhibit protein reconstitution. The results suggest that SBMs based on charged bolaamphiphiles might be an attractive platform for applications such as water purification and biosensors, where the stability and low defect rate of SBMs in diverse conditions are crucial for achieving desired performance.
authors.affiliation: Albert Katz International School for Desert Studies and Unit of Environmental Engineering, Ben-Gurion University of the Negev, Sde-Boqer, Israel.
authors.forename: Y
authors.initials: Y
authors.lastname: Kaufman
authors.forename: S
authors.initials: S
authors.lastname: Grinberg
authors.forename: C
authors.initials: C
authors.lastname: Linder
authors.forename: E
authors.initials: E
authors.lastname: Heldman
authors.forename: J
authors.initials: J
authors.lastname: Gilron
authors.forename: V
authors.initials: V
authors.lastname: Freger
mesh_terms: D020346:Aquaporins / Q000737:chemistry*; D040761:Biomimetic Materials / Q000737:chemistry*; D005663:Furans / Q000737:chemistry*; D008561:Membrane Fusion; D008567:Membranes, Artificial; D008823:Micelles; D009994:Osmolar Concentration; D010743:Phospholipids / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D011510:Proteolipids / Q000737:chemistry*; D011728:Pyridones / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D018724:Spinacia oleracea / Q000737:chemistry
chemical_list: D020346:Aquaporins; D005663:Furans; D008567:Membranes, Artificial; D008823:Micelles; C100932:PM28A protein, Spinacia oleracea; D010743:Phospholipids; D010940:Plant Proteins; D011510:Proteolipids; D011728:Pyridones; C543575:bolaamphiphile; C020485:proteoliposomes; D012822:Silicon Dioxide