title: DNA sensing by a Eu-binding peptide containing a proflavine unit.
abstract: Synthesis of a lanthanide-binding peptide (LBP) for the detection of double-stranded DNA is presented. A proflavine moiety was introduced into a high affinity LBP involving two unnatural chelating amino acids in the Ln ion coordination. The Eu(3+)-LBP complex is demonstrated to bind to ct-DNA and to sensitize Eu luminescence. The DNA binding process is effectively detected via the Eu-centered luminescence thanks to the intimate coupling between the LBP scaffold and DNA intercalating unit.
authors.affiliation: INAC, Service de Chimie Inorganique et Biologique (UMR_E 3 CEA UJF), Commissariat à l'Energie Atomique, 17 rue des martyrs, 38054 Grenoble cedex, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Ancel
authors.forename: Christelle
authors.initials: C
authors.lastname: Gateau
authors.forename: Colette
authors.initials: C
authors.lastname: Lebrun
authors.forename: Pascale
authors.initials: P
authors.lastname: Delangle
mesh_terms: D000818:Animals; D002614:Chelating Agents / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D005063:Europium / Q000737:chemistry*; D005453:Fluorescence; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D011370:Proflavine / Q000737:chemistry*
chemical_list: D002614:Chelating Agents; D009942:Organometallic Compounds; D010455:Peptides; D005063:Europium; D004247:DNA; D011370:Proflavine