title: Towards pancreatic cancer diagnosis using EIS biochips.
abstract: Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive and lethal cancers in Europe and the United States. It has a very low 5 years-survival rate and its diagnosis is often late and imprecise due to the lack of specificity of currently used markers for PDAC. As previously demonstrated PDAC patients' sera may contain autoantibodies towards phosphorylated α-enolase (ENOA), which in combination with other standard markers can increase specificity in diagnosis of PDAC. In this context we realized a microfluidic platform with integrated EIS biosensors. We achieved a specific antibodies detection by immobilizing onto electrodes peptides corresponding to a portion of ENOA. Phosphorylation of peptides was found to influence the recognition of antibodies in PDAC patients' sera detected by the developed biochip thus validating the EIS technique as a strong tool for quick, cost-saving and label-free analysis of serum samples. Biochip results are in agreement with those from traditional techniques, such as ELISA and western blot, but measurements are much more sensitive and specific, increasing the possibility of PDAC diagnosis. In addition this approach is faster and more reproducible compared to traditional techniques making the developed biochips ideal for a quick, cost-saving and label-free analysis of serum samples.
authors.affiliation: NNL Istituto Nanoscienze - CNR and Dipartimento di Matematica e Fisica Ennio De Giorgi, Scuola Superiore ISUFI, Università del Salento, Via per Arnesano, I-73100 Lecce, Italy. serena.chiriaco@unisalento.it
authors.forename: Maria Serena
authors.initials: MS
authors.lastname: Chiriacò
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Primiceri
authors.forename: Anna Grazia
authors.initials: AG
authors.lastname: Monteduro
authors.forename: Anna
authors.initials: A
authors.lastname: Bove
authors.forename: Stefano
authors.initials: S
authors.lastname: Leporatti
authors.forename: Michela
authors.initials: M
authors.lastname: Capello
authors.forename: Sammy
authors.initials: S
authors.lastname: Ferri-Borgogno
authors.forename: Ross
authors.initials: R
authors.lastname: Rinaldi
authors.forename: Francesco
authors.initials: F
authors.lastname: Novelli
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Maruccio
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D021441:Carcinoma, Pancreatic Ductal / Q000097:blood / Q000175:diagnosis*; D058266:Dielectric Spectroscopy / Q000295:instrumentation / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D010190:Pancreatic Neoplasms / Q000097:blood / Q000175:diagnosis*