title: Chemiluminescence detection of glucose using Ce(IV) oxidation in a batch system.
abstract: A chemiluminescence method for the determination of glucose (GC) using a batch system made in our laboratory is proposed.  The method is based on measures of the chemiluminesence intensity from a batch of sample GC solutions following the injection of an (NH(4))(2)[Ce(NO(3))(6)]-[Ru(bpy)(3)]Cl(2) solution.  The effect of the Ru complex concentration on chemiluminescence was studied.  The detection limit was 0.5 × 10(-8) M, and the linear calibration curve reached 10(-7) M.  The Ru complex was selected on the basis of its desirable performance compared to those of other photosensitizers.  However, flow-injection analysis (FIA) using the Ce-Ru complex reagent was not applicable for the detection of GC.
authors.affiliation: Separation Science Division, Faculty of Science, Okayama University of Science, Okayama, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Nagai
authors.forename: Wakayo
authors.initials: W
authors.lastname: Miyahara
authors.forename: Honami
authors.initials: H
authors.lastname: Sagawa
authors.forename: Shigeo
authors.initials: S
authors.lastname: Yamazaki
authors.forename: Keiitsu
authors.initials: K
authors.lastname: Saito
mesh_terms: D002563:Cerium / Q000737:chemistry*; D004867:Equipment Design; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D005947:Glucose / Q000032:analysis*; D057230:Limit of Detection; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D009566:Nitrates / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D012996:Solutions
chemical_list: D049408:Luminescent Agents; D009566:Nitrates; D009942:Organometallic Compounds; D012996:Solutions; C532772:cerium(IV) ammonium nitrate; C415768:tris(2,2'-bipyridyl)ruthenium(II); D002563:Cerium; D005947:Glucose