title: Determination of soyasaponins I and βg in raw and cooked legumes by solid phase extraction (SPE) coupled to liquid chromatography (LC)-mass spectrometry (MS) and assessment of their bioaccessibility by an in vitro digestion model.
abstract: Legumes contain a rich variety of phytochemicals as soyasaponins, triterpenoidal glycosides that possess multiple health-promoting properties, such as lowering of cholesterol. In this work, the quantification of soyasaponins I and βg in 60 raw and cooked legumes by using a solid phase extraction (SPE) coupled to a liquid chromatography (LC)-mass spectrometry (MS) method was carried out. Results showed that lentils are a good source of soyasaponins, with a content of soyasaponin I that ranged from 636 to 735 mg kg(-1) and of soyasaponin βg from 672 to 1807 mg kg(-1). The cooking process produced a small loss of soyasaponins in water, that is, 4.8-8.7%, and partially converted soyasaponin βg into soyasaponin I. In addition, the bioaccessibility of soyasaponins I in lentils was studied; the values ranged from 8.9 ± 0.3 to 10.6 ± 1.1% in the duodenal compartment. On the basis of these results, soyasaponins could be effective in lowering exogenous cholesterol.
authors.affiliation: School of Pharmacy, University of Camerino, Camerino, Italy.
authors.forename: Gianni
authors.initials: G
authors.lastname: Sagratini
authors.forename: Giovanni
authors.initials: G
authors.lastname: Caprioli
authors.forename: Filippo
authors.initials: F
authors.lastname: Maggi
authors.forename: Guillermina
authors.initials: G
authors.lastname: Font
authors.forename: Dario
authors.initials: D
authors.lastname: Giardinà
authors.forename: Jordi
authors.initials: J
authors.lastname: Mañes
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Meca
authors.forename: Massimo
authors.initials: M
authors.lastname: Ricciutelli
authors.forename: Veronica
authors.initials: V
authors.lastname: Sirocchi
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Torregiani
authors.forename: Sauro
authors.initials: S
authors.lastname: Vittori
mesh_terms: D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004063:Digestion; D007887:Fabaceae / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D012503:Saponins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D012503:Saponins; C089346:soyasaponin beta g; C056684:soyasaponin I; D009828:Oleanolic Acid