title: Antimutagenic effect of aqueous extract from Agaricus brasiliensis on culture of human lymphocytes.
abstract: The mushroom Agaricus brasiliensis (sun mushroom), native from the southeast of Brazil, is well known by its medicinal properties that include effects on diabetes, cholesterol levels, and osteoporosis. The antimutagenic effects of A. brasiliensis has been investigated recently and revealed some controversial results depending on the temperature by which the A. brasiliensis tea is obtained. In the present study, we evaluated the effect of the A. brasiliensis extract prepared in two different temperatures, 4°C and 25°C, on the doxorubicin-induced DNA strand breaks and chromosomal aberrations (CAs) in human lymphocytes. The results demonstrated that A. brasiliensis was able to reduce the DXR-induced DNA damage in both temperatures; however, the CA test was more sensitive to demonstrate a better reduction when the cells were treated with an extract obtained at 25°C. A. brasiliensis extract obtained in different temperatures exhibited antigenotoxic and anticlastogenic effects in human lymphocytes.
authors.affiliation: Department of Microbiology and Parasitology, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil. phgameiro@gmail.com
authors.forename: Paula H
authors.initials: PH
authors.lastname: Gameiro
authors.forename: José S
authors.initials: JS
authors.lastname: Nascimento
authors.forename: Beatriz H G
authors.initials: BH
authors.lastname: Rocha
authors.forename: Clause F B
authors.initials: CF
authors.lastname: Piana
authors.forename: Raquel A
authors.initials: RA
authors.lastname: Santos
authors.forename: Catarina S
authors.initials: CS
authors.lastname: Takahashi
mesh_terms: D000364:Agaricus / Q000737:chemistry*; D016587:Antimutagenic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*
chemical_list: D016587:Antimutagenic Agents