title: In vitro inhibitory and pro-apoptotic effect of Stellera chamaejasme L extract on human lung cancer cell line NCI-H157.
abstract: OBJECTIVE
  To investigate the inhibitory and pro-apoptotic effect of Stellera Chamaejasme L extract (ESC) in vitro.
  
  
  METHODS
  ESC was first extracted with ethanol, and then washed using a polyamide column with 60% ethanol. ESC was then decompressively recycled and vacuum dried at room temperature to obtain active fractions. Subsequently, the cytotoxic and apoptotic effects of ESC on NCI-H157 human lung cancer cells were determined.
  
  
  RESULTS
  The results showed that ESC was rich in isomers of Chamaejasminor, neochamaejasmine and Sikokianin. ESC had significant cytotoxicity against NCI-H157 cells, with an IC50 of approximately 18.50 microg x mL(-). ESC caused significant increase in total apoptotic rate, the activity of caspase 3 and 8,
  
  
  CONCLUSIONS
  The inhibitory effect of ESC on NCI-H157 tumor cells might partly be attributed to its apoptotic induction through activation of the Fas death receptor pathway.
authors.affiliation: Beijing YouAn Hospital, Capital Medical University, Beijing 100069, China.
authors.forename: Xiaoni
authors.initials: X
authors.lastname: Liu
authors.forename: Yujie
authors.initials: Y
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Weng
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Xiaoxin
authors.initials: X
authors.lastname: Zhu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053181:Caspase 8 / Q000235:genetics / Q000378:metabolism; D053453:Caspase 9 / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology / Q000235:genetics / Q000503:physiopathology*; D029645:Thymelaeaceae / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9