title: Application of N-halogeno-N-sodiobenzenesulfonamide reagents to the selective detection of 5-methylcytosine in DNA sequences.
abstract: To surmount the challenges of the locus determination and accurate quantification of 5-methyl-2'-deoxycytidine ((5Me)dC) in DNA fragments that contain multiple (5Me)dC residues, we designed and synthesized two N-halogeno-N-sodiobenzenesulfonamide reagents that provide a new chemical method for probing (5Me)dC in DNA sequences. When the strategy we provided was combined with β-glucosyltransferase, (5Me)dC could be distinguished from 5-hydroxymethyl-2'-deoxycytidine ((5hm)dC) and deoxycytidine (dC) through the introduction of a glucose moiety to the hydroxyl group of (5hm)dC.
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of the Ministry of Education, Wuhan University, Wuhan, Hubei 430072, PR China.
authors.forename: Tianlu
authors.initials: T
authors.lastname: Wang
authors.forename: Tingting
authors.initials: T
authors.lastname: Hong
authors.forename: Tun
authors.initials: T
authors.lastname: Tang
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Zhai
authors.forename: Xiwen
authors.initials: X
authors.lastname: Xing
authors.forename: Wuxiang
authors.initials: W
authors.lastname: Mao
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Zheng
authors.forename: Liang
authors.initials: L
authors.lastname: Xu
authors.forename: Jinjun
authors.initials: J
authors.lastname: Wu
authors.forename: Xiaocheng
authors.initials: X
authors.lastname: Weng
authors.forename: Shaoru
authors.initials: S
authors.lastname: Wang
authors.forename: Tian
authors.initials: T
authors.lastname: Tian
authors.forename: Bifeng
authors.initials: B
authors.lastname: Yuan
authors.forename: Bing
authors.initials: B
authors.lastname: Huang
authors.forename: Lin
authors.initials: L
authors.lastname: Zhuang
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
mesh_terms: D044503:5-Methylcytosine / Q000032:analysis*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D009574:Nitro Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D013449:Sulfonamides / Q000138:chemical synthesis / Q000737:chemistry*; D013696:Temperature; D000096926:Benzenesulfonamides
chemical_list: D044503:5-Methylcytosine; D004247:DNA; D009574:Nitro Compounds; D013449:Sulfonamides; C582351:N-sodio-N-bromo-m-nitrobenzenesulfonamide; D000096926:Benzenesulfonamides; C582350:N-sodio-N-chloro-p-nitrobenzenesulfonamide