title: An IκB homologue (FcCactus) in Chinese shrimp Fenneropenaeus chinensis.
abstract: This study firstly reports the characterization of a functional IκB homologue, FcCactus in Chinese shrimp Fenneropenaeus chinensis. The full length cDNA of FcCactus consists of a 1359 bp open reading frame (ORF) encoding a 453 amino acid protein with a predicted molecular weight (MW) of 48.46 kDa and theoretical pI of 5.23. Phylogenetic analysis and multiple alignments revealed that the deduced amino acid sequence of FcCactus cDNA had high similarities to Cactus or IκB reported in seven other arthropods. Genomic DNA sequence of FcCactus was also obtained with a length of more than 17698 bp and constituted of seven exons and six introns. Analysis on 5'-upstream regulatory region of its DNA sequence revealed that it contained the core promoter sequence with the TATA-box and transcription start site existing in it; furthermore, various transcription factor binding sites (HSF, Hb, BR-C Z, Dfd, CF2-II, Croc, Ttk, Dorsal, and c-Rel) were predicted. Spatial expression profiles showed that FcCactus mRNA had the highest expression level in muscle, hemocytes, heart and lymphoid organ. Gram-positive bacteria (Micrococcus lysodeikticus) and Gram-negative bacteria (Vibrio anguillarium) injection to shrimp caused the modulation of FcCactus at the transcription level. DsRNAi (double-strand RNA interference) approach was used to study the function of FcCactus and the data showed that FcCactus could regulate the expression of different antimicrobial peptides (AMPs) and antiviral factor (AV). The present data showed that FcCactus might play important roles in regulating the immune response of shrimp.
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Wang
authors.forename: Fuhua
authors.initials: F
authors.lastname: Li
authors.forename: Shihao
authors.initials: S
authors.lastname: Li
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Chi
authors.forename: Rong
authors.initials: R
authors.lastname: Wen
authors.forename: Ningning
authors.initials: N
authors.lastname: Feng
authors.forename: Jianhai
authors.initials: J
authors.lastname: Xiang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D018076:DNA, Complementary; D020869:Gene Expression Profiling; D020672:I-kappa B Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D008837:Micrococcus / Q000276:immunology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D033561:Penaeidae / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA; D014733:Vibrio / Q000276:immunology
chemical_list: D018076:DNA, Complementary; D020672:I-kappa B Proteins; D012333:RNA, Messenger; D004247:DNA