title: Sialic acid capture-and-release and LC-MS(n) analysis of glycopeptides.
abstract: Extracellular glycoproteins frequently carry terminal sialic acids on their N-linked and/or O-linked glycan structures. In this chapter a sialic acid specific capture-and-release protocol for the enrichment of N- and O-glycopeptides originating from glycoproteins in complex biological samples is described. The enriched glycopeptides are subjected to reversed phase liquid chromatography (LC) interfaced with electrospray ionization and multistage tandem mass spectrometry (MS(n)). The glycopeptide precursor ions are fragmented by collision-induced dissociation (CID) for analysis of the glycan parts in the MS(2) spectra. Further fragmentation (i.e., MS(3)) of deglycosylated peptide ions results in peptide backbone fragmentation, which is used in protein database searches to identify protein sequences. For O-glycopeptides the use of both CID and electron capture dissociation (ECD) fragmentation of the peptide backbone with intact glycans still attached are used to pinpoint the glycosylation sites of glycopeptides containing several Ser/Thr residues. The step-by-step protocols for fragmentation analyses of O- and N-glycopeptides enriched from human cerebrospinal fluid are described.
authors.affiliation: Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, the Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden. jonas.nilsson@clinchem.gu.se
authors.forename: Jonas
authors.initials: J
authors.lastname: Nilsson
authors.forename: Göran
authors.initials: G
authors.lastname: Larson
mesh_terms: D000595:Amino Acid Sequence; D002853:Chromatography, Liquid / Q000379:methods*; D006020:Glycopeptides / Q000032:analysis* / Q000134:cerebrospinal fluid / Q000737:chemistry / Q000302:isolation & purification*; D006031:Glycosylation; D006801:Humans; D006834:Hydrazines / Q000737:chemistry; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid / Q000737:chemistry*; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010504:Periodic Acid / Q000737:chemistry; D059748:Proteolysis; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014357:Trypsin / Q000378:metabolism
chemical_list: D006020:Glycopeptides; D006834:Hydrazines; D010446:Peptide Fragments; D010504:Periodic Acid; C029424:hydrazine; C009288:metaperiodate; D014357:Trypsin; D019158:N-Acetylneuraminic Acid