title: Aquaporin 1 plays an important role in myocardial edema caused by cardiopulmonary             bypass surgery in goat.
abstract: Myocardial stunning, which is closely related to myocardial edema, is a             severe complication that may occur following cardiac surgery. In this study, we             examined the expression of aquaporin 1 (AQP1) and Connexin 43 (Cx43) following             cardiopulmonary bypass (CPB) surgery in goats. We assessed myocardial muscle tissue             water content according to changes in dry-wet weight. Our results showed that             AQP1 expression and myocardial muscle tissue water content increased significantly             6 h after CPB surgery, reaching peak levels 48 h after surgery; additionally,             the protein expression of Cx43 was inversely correlated with AQP1 expression.             Overexpression of AQP1 during CPB surgery enhanced the degree of myocardial edema,             whereas the addition of water channel protein inhibitor Hg2+ in cold crystalloid             cardioplegia and knockdown of AQP1 during surgery weakened the degree of myocardial             edema. These findings revealed that the severity of myocardial edema after CPB             surgery is correlated with AQP1 protein expression levels, suggesting the important             role played by AQP1 protein in the regulation of Cx43 in the pathological progression             of myocardial edema.
authors.affiliation: Department of Cardiothoracic Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, PR China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Yan
authors.forename: Jianbin
authors.initials: J
authors.lastname: Huang
authors.forename: Fangbao
authors.initials: F
authors.lastname: Ding
authors.forename: Ju
authors.initials: J
authors.lastname: Mei
authors.forename: Jiaquan
authors.initials: J
authors.lastname: Zhu
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.forename: Kun
authors.initials: K
authors.lastname: Sun
mesh_terms: D000818:Animals; D051398:Aquaporin 1 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D002314:Cardioplegic Solutions; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D018031:Connexin 43 / Q000378:metabolism*; D004489:Edema, Cardiac / Q000209:etiology / Q000378:metabolism*; D006041:Goats; D006352:Heart Ventricles / Q000378:metabolism; D008628:Mercury / Q000494:pharmacology; D009206:Myocardium / Q000378:metabolism; D014867:Water
chemical_list: D002314:Cardioplegic Solutions; D018031:Connexin 43; D014867:Water; D051398:Aquaporin 1; D008628:Mercury