title: Immunomodulatory effects of a probiotic drink containing Lactobacillus casei Shirota in healthy older volunteers.
abstract: OBJECTIVE
  There is growing evidence that probiotics confer health benefits to the host by modulating immune function, especially in older people, where immunosenescence is a feature even of healthy ageing. The aim of this study was to investigate the effect of a probiotic drink containing Lactobacillus casei Shirota (LcS) on immune function in a healthy non-immunocompromised older population.
  
  
  METHODS
  Thirty healthy old volunteers were recruited into a randomized placebo-controlled, single-blind crossover study. The volunteers were supplemented with the probiotic drink containing 1.3 × 10(10) CFU LcS or skimmed milk per day for 4 weeks, followed by 4 weeks of washout and were crossed over to the other treatment. Peripheral blood and saliva samples were collected at baseline and end of each treatment.
  
  
  RESULTS
  Probiotic consumption was associated with a significant increase in natural killer (NK) cell activity relative to baseline and a significant decrease in the mean fluorescence intensity of CD25 expression in the resting T cells compared with placebo. Additionally, there was a trend towards an increased ratio of IL-10 to IL-12 relative to baseline after LcS intake.
  
  
  CONCLUSIONS
  Consumption of a probiotic drink containing LcS improved NK cell activity and tended to produce a more anti-inflammatory cytokine profile in an older population.
authors.affiliation: Department of Food and Nutritional Sciences, The University of Reading, Whiteknights, PO Box 226, Reading, RG6 6AP, UK, h.dong@reading.ac.uk.
authors.forename: Honglin
authors.initials: H
authors.lastname: Dong
authors.forename: Ian
authors.initials: I
authors.lastname: Rowland
authors.forename: Linda V
authors.initials: LV
authors.lastname: Thomas
authors.forename: Parveen
authors.initials: P
authors.lastname: Yaqoob
mesh_terms: D000368:Aged; D001628:Beverages; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D049109:Cell Proliferation; D018592:Cross-Over Studies; D016207:Cytokines / Q000378:metabolism; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D007113:Immunity, Innate / Q000502:physiology; D016753:Interleukin-10 / Q000097:blood; D018664:Interleukin-12 / Q000097:blood; D053645:Interleukin-2 Receptor alpha Subunit / Q000235:genetics / Q000378:metabolism; D007694:Killer Cells, Natural / Q000378:metabolism; D007780:Lacticaseibacillus casei / Q000378:metabolism*; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D010586:Phagocytes / Q000378:metabolism; D010641:Phenotype; D019936:Probiotics / Q000008:administration & dosage*; D016037:Single-Blind Method; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D016207:Cytokines; C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit; D008055:Lipids; D016753:Interleukin-10; D018664:Interleukin-12