title: Increased expression of phospholipase Dα1 in guard cells decreases water loss with improved seed production under drought in Brassica napus.
abstract: The activation of phospholipase Dα1 (PLDα1) produces lipid messenger phosphatidic acid and promotes stomatal closure in Arabidopsis. To explore the use of the PLDα1-mediated signalling towards decreasing water loss in crop plants, we introduced Arabidopsis PLDα1 under the control of a guard cell-specific promoter AtKatIpro into two canola (Brassica napus) cultivars. Multiple AtKatIpro ::PLDα1 lines in each cultivar displayed decreased water loss and improved biomass accumulation under hyperosmotic stress conditions, including drought and high salinity. Moreover, AtKatIpro ::PLDα1 plants produced more seeds than did WT plants in fields under drought. The results indicate that the guard cell-specific expression of PLDα1 has the potential to improve crop yield by enhancing drought tolerance.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, College of Life Sciences and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Shaoping
authors.initials: S
authors.lastname: Lu
authors.forename: Sung C
authors.initials: SC
authors.lastname: Bahn
authors.forename: Gang
authors.initials: G
authors.lastname: Qu
authors.forename: Haiying
authors.initials: H
authors.lastname: Qin
authors.forename: Yue
authors.initials: Y
authors.lastname: Hong
authors.forename: Qingpeng
authors.initials: Q
authors.lastname: Xu
authors.forename: Yongming
authors.initials: Y
authors.lastname: Zhou
authors.forename: Yueyun
authors.initials: Y
authors.lastname: Hong
authors.forename: Xuemin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000040:Abscisic Acid / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics; D029688:Brassica napus / Q000201:enzymology*; D055864:Droughts; D035264:Flowers / Q000502:physiology; D010739:Phospholipase D / Q000502:physiology*; D054046:Plant Stomata / Q000201:enzymology*; D024661:Potassium Channels, Inwardly Rectifying / Q000235:genetics; D011401:Promoter Regions, Genetic; D036226:Seedlings / Q000254:growth & development; D012639:Seeds / Q000254:growth & development*; D012965:Sodium Chloride / Q000378:metabolism; D014867:Water / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; C501210:KAT1 protein, Arabidopsis; D024661:Potassium Channels, Inwardly Rectifying; D014867:Water; D012965:Sodium Chloride; D000040:Abscisic Acid; D010739:Phospholipase D