title: A facile method for the preparation of monodisperse beads with uniform pore sizes for cell culture.
abstract: This paper describes a facile method for the preparation of porous gelatin beads with uniform pore sizes using a simple fluidic device and their application as supporting materials for cell culture. An aqueous gelatin droplet containing many uniform toluene droplets, produced in the fluidic device, is dropped into liquid nitrogen for instant freezing and the small toluene droplets evolve into pores in the gelatin beads after removal of toluene and then freeze-drying. The porous gelatin beads exhibit a uniform pore size and monodisperse diameter as well as large open pores at the surface. Fluorescence microscopy images of fibroblast-loaded gelatin beads confirm the attachment and proliferation of the cells throughout the porous gelatin beads.
authors.affiliation: Department of Biotechnology, The Catholic University of Korea, Gyeonggi-do, Republic of Korea.
authors.forename: Seung-Kwan
authors.initials: SK
authors.lastname: Moon
authors.forename: Myeong-Jin
authors.initials: MJ
authors.lastname: Oh
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Paik
authors.forename: Tae-Kyung
authors.initials: TK
authors.lastname: Ryu
authors.forename: Kyeongsoon
authors.initials: K
authors.lastname: Park
authors.forename: Sung-Eun
authors.initials: SE
authors.lastname: Kim
authors.forename: Jong-Hoon
authors.initials: JH
authors.lastname: Park
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Sung-Wook
authors.initials: SW
authors.lastname: Choi
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods*; D016062:Porosity
chemical_list: D001672:Biocompatible Materials