title: Cell-specific expression profiling of rare cell types as exemplified by its impact on our understanding of female gametophyte development.
abstract: Expression profiling of single cells can yield insights into cell specification, cellular differentiation processes, and cell type-specific responses to environmental stimuli. Recent work has established excellent tools to perform genome-wide expression studies of individual cell types, even if the cells of interest occur at low frequency within an organ. We review the advances and impact of gene expression studies of rare cell types, as exemplified by recently gained insights into the development and function of the angiosperm female gametophyte. The detailed transcriptional characterization of different stages during female gametophyte development has significantly helped to improve our understanding of cellular specification or cell-cell communication processes. Next-generation sequencing approaches--used increasingly for expression profiling--will now allow for comparative approaches that focus on agriculturally, ecologically or evolutionarily relevant aspects of plant reproduction.
authors.affiliation: Institute of Evolutionary Biology and Environmental Studies & Zürich-Basel Plant Science Center, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland.
authors.forename: Samuel E
authors.initials: SE
authors.lastname: Wuest
authors.forename: Marc W
authors.initials: MW
authors.lastname: Schmid
authors.forename: Ueli
authors.initials: U
authors.lastname: Grossniklaus
mesh_terms: D002454:Cell Differentiation; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D059014:High-Throughput Nucleotide Sequencing; D019684:Magnoliopsida / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D009928:Organ Specificity; D056771:Ovule / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D012098:Reproduction; D017422:Sequence Analysis, DNA; D059010:Single-Cell Analysis / Q000379:methods*