title: Proteome map of the neural complex of the tunicate Ciona intestinalis, the closest living relative to vertebrates.
abstract: Ciona intestinalis (the common sea squirt) is the closest living chordate relative to vertebrates with cosmopolitan presence worldwide. It has a relatively simple nervous system and development, making it a widely studied alternative model system in neuroscience and developmental biology. The use of Ciona as a model organism has increased significantly after the draft genome was published. In this study, we describe the first proteome map of the neural complex of C. intestinalis. A total of 544 proteins were identified based on 1DE and 2DE FTMS/ITMSMS analyses. Proteins were annotated against the Ciona database and analyzed to predict their molecular functions, roles in biological processes, and position in constructed network pathways. The identified Ciona neural complex proteome was found to map onto vertebrate nervous system pathways, including cytoskeleton remodeling neurofilaments, cell adhesion through the histamine receptor signaling pathway, γ-aminobutyric acid-A receptor life cycle neurophysiological process, glycolysis, and amino acid metabolism. The proteome map of the Ciona neural complex is the first step toward a better understanding of several important processes, including the evolution and regeneration capacity of the Ciona nervous system.
authors.affiliation: CSIR-Centre for Cellular and Molecular Biology (CCMB), Hyderabad, Andhra Pradesh, India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Saxena
authors.forename: Sam
authors.initials: S
authors.lastname: Dupont
authors.forename: Vuppalapaty
authors.initials: V
authors.lastname: Meghah
authors.forename: Mula G Meena
authors.initials: MG
authors.lastname: Lakshmi
authors.forename: Sachin K
authors.initials: SK
authors.lastname: Singh
authors.forename: Cherukuvada V Brahmendra
authors.initials: CV
authors.lastname: Swamy
authors.forename: Mohammed M
authors.initials: MM
authors.lastname: Idris
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D002938:Ciona intestinalis / Q000737:chemistry* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D057166:High-Throughput Screening Assays; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000737:chemistry; D009420:Nervous System / Q000737:chemistry / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics; D053719:Tandem Mass Spectrometry
chemical_list: D009419:Nerve Tissue Proteins; D020543:Proteome