title: [Isolation and amplification cDNA of 8H07 gene conservative region of nematode Heterodera schachtii with high relationship to its rape homolog].
abstract: Original method of small regulatory si/miRNA isolation from plant cells was elaborated. PCR amplification of fragment cDNA 8H07 nematode Heterodera schachtii gene was carried out. Using Northern-blot method hybridization of plant si/miRNA with cDNA fragment of conservative region 8H07 gene the presence of their high homology is found out. The amplified cDNA fragment of nematode 8H07 gene in future will be used for creation recombinant gene with complementary antisense dsRNA sequence for increasing resistance of rape plants to parasitic nematodes.
authors.forename: V A
authors.initials: VA
authors.lastname: Tsyhankova
authors.forename: Ia V
authors.initials: IaV
authors.lastname: Andrusevych
authors.forename: S P
authors.initials: SP
authors.lastname: Ponomarenko
authors.forename: A P
authors.initials: AP
authors.lastname: Halkin
authors.forename: Ia B
authors.initials: IaB
authors.lastname: Blium
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D029741:Brassica rapa / Q000187:drug effects / Q000235:genetics* / Q000469:parasitology; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D017238:Genes, Helminth; D006790:Host-Parasite Interactions / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics* / Q000302:isolation & purification; D010937:Plant Growth Regulators / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D018749:RNA, Plant / Q000235:genetics* / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D014431:Tylenchoidea / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D018509:DNA, Helminth; D035683:MicroRNAs; D010937:Plant Growth Regulators; D018749:RNA, Plant