title: Screening promoters for Anthurium transformation using transient expression.
abstract: KEY MESSAGE                 :                                     There are multiple publications on                   Anthurium                   transformation, yet a commercial product has not been achieved. This may be due to use of non-optimum promoters here we address this problem.                                                 Different promoters and tissue types were evaluated for transient β-glucuronidase (GUS) expression in Anthurium                   andraeanum Hort. 'Marian Seefurth' following microprojectile bombardment. Plasmids containing the Ubiquitin 2, Actin 1, Cytochrome C1 from rice, Ubiquitin 1 from maize and 35S promoter from Cauliflower Mosaic Virus fused to a GUS reporter gene were bombarded into in vitro grown anthurium lamina, somatic embryos and roots. The number of GUS foci and the intensity of GUS expression were evaluated for each construct. Ubiquitin promoters from rice and maize resulted in the highest number of expressing cells in all tissues examined. Due to the slow growth of anthurium plants, development of transgenic anthurium plants takes years. This research has rapidly identified multiple promoters that express in various anthurium tissues facilitating the development of transformation vectors for the expression of desirable traits in anthurium plants.
authors.affiliation: USDA, ARS, Pacific Basin Agricultural Research Center, 64 Nowelo Street, Hilo, HI 96720, USA. tracie.matsumoto@ars.usda.gov
authors.forename: Tracie K
authors.initials: TK
authors.lastname: Matsumoto
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Keith
authors.forename: Roxana Y M
authors.initials: RY
authors.lastname: Cabos
authors.forename: Jon Y
authors.initials: JY
authors.lastname: Suzuki
authors.forename: Dennis
authors.initials: D
authors.lastname: Gonsalves
authors.forename: Roger
authors.initials: R
authors.lastname: Thilmony
mesh_terms: D029064:Araceae / Q000235:genetics*; D017796:Caulimovirus / Q000235:genetics; D018929:Cell Culture Techniques; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors; D005966:Glucuronidase; D009928:Organ Specificity; D012275:Oryza / Q000235:genetics; D018517:Plant Roots / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins; D003313:Zea mays / Q000235:genetics
chemical_list: D011993:Recombinant Fusion Proteins; D005966:Glucuronidase