title: The effect of laminin-1-doped nanoroughened implant surfaces: gene expression and morphological evaluation.
abstract: Aim. This study aimed to observe the morphological and molecular effect of laminin-1 doping to nanostructured implant surfaces in a rabbit model. Materials and Methods. Nanostructured implants were coated with laminin-1 (test; dilution, 100 μg/mL) and inserted into the rabbit tibiae. Noncoated implants were used as controls. After 2 weeks of healing, the implants were removed and subjected to morphological analysis using scanning electron microscopy (SEM) and gene expression analysis using the real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Results. SEM revealed bony tissue attachment for both control and test implants. Real-time RT-PCR analysis showed that the expression of osteoblast markers RUNX-2, osteocalcin, alkaline phosphatase, and collagen I was higher (1.62-fold, 1.53-fold, 1.97-fold, and 1.04-fold, resp.) for the implants modified by laminin-1 relative to the control. All osteoclast markers investigated in the study presented higher expression on the test implants than controls as follows: tartrate-resistant acid phosphatase (1.67-fold), calcitonin receptor (1.35-fold), and ATPase (1.25-fold). The test implants demonstrated higher expression of inflammatory markers interleukin-10 (1.53-fold) and tumour necrosis factor-α (1.61-fold) relative to controls. Conclusion. The protein-doped surface showed higher gene expression of typical genes involved in the osseointegration cascade than the control surface.
authors.affiliation: Department of Prosthodontics, Faculty of Odontology, Malmö University, 205 06 Malmö, Sweden ; Division of Periodontology, Department of Oral Diagnosis and Surgery, School of Dentistry, UNESP, São Paulo State University, 01049-010 Araraquara, SP, Brazil.
authors.forename: Humberto Osvaldo
authors.initials: HO
authors.lastname: Schwartz-Filho
authors.forename: Kostas
authors.initials: K
authors.lastname: Bougas
authors.forename: Paulo G
authors.initials: PG
authors.lastname: Coelho
authors.forename: Ying
authors.initials: Y
authors.lastname: Xue
authors.forename: Mariko
authors.initials: M
authors.lastname: Hayashi
authors.forename: Rafael Silveira
authors.initials: RS
authors.lastname: Faeda
authors.forename: Rosemary Adriana Chiérici
authors.initials: RA
authors.lastname: Marcantonio
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ono
authors.forename: Fumio
authors.initials: F
authors.lastname: Kobayashi
authors.forename: Kamal
authors.initials: K
authors.lastname: Mustafa
authors.forename: Ann
authors.initials: A
authors.lastname: Wennerberg
authors.forename: Ryo
authors.initials: R
authors.lastname: Jimbo