title: Response of plasma fatty acid profiles to changes in dietary n-3 fatty acids and its correlation with erythrocyte fatty acid profiles in dogs.
abstract: An elevated level of long-chain n-3 fatty acids (FA) in tissue membranes has a positive influence on the progression and treatment of many diseases. Therefore, dietary supplementation of n-3 FA is recommended in some diseases. Even though n-3 FA are absorbed readily from the diet, their incorporation into tissues may be compromised in diseased animals. In a clinical setting, it is desirable to monitor the success of dietary intervention. Plasma FA as well as erythrocyte membrane (EM) FA can be used to monitor dietary FA intake. This study compares FA from EM and plasma with regard to their reaction time and reliability for monitoring dietary changes of tissue FA profiles in dogs. Thirty dogs were divided into three groups and fed for 12 weeks. The control group (CONT) was fed a commercial standard diet low in n-3 FA. One group received the standard diet and 85 mg/kg body weight of a docosahexaenoic acid (DHA) concentrate (ADD). The third group was fed a commercial dog food containing fish oil (FO), which is rich in eicosapentaenoic acid (EPA). EM and plasma FA profiles were analysed by GC separately. Data on EM FA were published recently. n-3 FA in plasma reached the new level after 2 weeks (8 weeks in EM). Dietary differences between DHA and EPA are obvious after 1 week already. The concomitant decrease in plasma n-6 FA differed between ADD and FO. In general, the correlation of n-6 FA between plasma and EM was low. We therefore conclude that analysis of plasma FA is sufficient for monitoring a diet-induced increase in tissue n-3 FA in dogs. However, EM FA should be analysed if the effect of dietary intervention on tissue n-6 FA is important.
authors.affiliation: Institute of Physiological Chemistry, Faculty of Veterinary Medicine, University of, Leipzig, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Stoeckel
authors.forename: L
authors.initials: L
authors.lastname: Bachmann
authors.forename: G
authors.initials: G
authors.lastname: Dobeleit
authors.forename: H
authors.initials: H
authors.lastname: Fuhrmann
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D004032:Diet / Q000662:veterinary*; D004041:Dietary Fats / Q000032:analysis / Q000494:pharmacology; D019587:Dietary Supplements; D004285:Dogs / Q000097:blood* / Q000378:metabolism; D004912:Erythrocytes / Q000737:chemistry / Q000378:metabolism*; D005227:Fatty Acids / Q000097:blood* / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids
keywords: biomarker; cancer; docosahexaenoic acid; eicosapentaenoic acid; polyunsaturated fatty acids