title: Acanthopanax senticosus extracts have a protective effect on Drosophila gut immunity.
abstract: BACKGROUND
  Aanthopanax senticosus (A. senticosus) Harms is a classical adaptogenic agent used in China. It has been applied as an analeptic aid to improve weakened physical status. However, little is known about the effects of A. senticosus on inflammatory disease processes.
  
  
  METHODS
  Flies fed with standard cornmeal-yeast medium were used as controls, and the treatment groups contained 10% of A. senticosus aqueous extracts (root or fruit) in standard medium. Survival rate was performed by feeding a vial containing five layers of filter paper hydrated with 5% sucrose solution contaminated with pathogenic or toxic compounds. Imaging of the guts was viewed under the microscope. Death cells were detected by 7-AAD staining.
  
  
  RESULTS
  The A. senticosus extract improved the survival rate, attenuated the death of intestinal epithelial cells, promoted the expression of antimicrobial peptide genes, and decreased the formation of melanotic masses. Moreover, our results indicated that the protective effect of fruit is much higher than that of root extracts.
  
  
  CONCLUSIONS
  A. senticosus extracts have a protective effect on Drosophila gut immunity and stress response, and may contribute to the prevention of inflammatory diseases induced by pathogenic and toxic compounds.
authors.affiliation: College of Life Sciences, Northeast Forestry University, Harbin, China.
authors.forename: Wenjia
authors.initials: W
authors.lastname: Li
authors.forename: Qiuxiang
authors.initials: Q
authors.lastname: Luo
authors.forename: Li Hua
authors.initials: LH
authors.lastname: Jin
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics; D001424:Bacterial Infections / Q000517:prevention & control; D052982:Beauveria / Q000187:drug effects / Q000472:pathogenicity; D016923:Cell Death / Q000187:drug effects; D004330:Drosophila; D028001:Eleutherococcus; D004847:Epithelial Cells / Q000187:drug effects; D005260:Female; D005638:Fruit; D007422:Intestines / Q000187:drug effects* / Q000276:immunology / Q000382:microbiology; D008297:Male; D016982:Micrococcus luteus / Q000187:drug effects / Q000472:pathogenicity; D009181:Mycoses / Q000517:prevention & control; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots; D020011:Protective Agents / Q000494:pharmacology*; D013172:Spores, Fungal / Q000187:drug effects / Q000472:pathogenicity; D013312:Stress, Physiological / Q000187:drug effects
chemical_list: D023181:Antimicrobial Cationic Peptides; D010936:Plant Extracts; D020011:Protective Agents