title: Phage-displayed specific polypeptide antigens induce significant protective immunity against Trichinella spiralis infection in BALB/c mice.
abstract: Trichinellosis is a public health problem and is considered an emerging/re-emerging disease in various countries. The etiological agent of trichinellosis is the nematode Trichinella, which infects humans, domestic animals and wildlife. A veterinary vaccine could be an option to control the disease in domestic animals. Although several vaccine candidates have shown promising results, a vaccine against trichinellosis remains unavailable to date. Phage particles are especially ideal vaccine delivery vehicles because they do not interfere with the immune response against the displayed peptide antigens, and, if anything, are more likely to efficiently direct antigen expression to professional antigen-presenting cells. In this study, Tsp10 polypeptide, which was encoded by a cDNA fragment of Trichinella spiralis intestinal infective larvae and was found to bind to normal mouse intestinal cells, was displayed on the surface of T7 phage. Anti-Tsp10 antibodies were able to recognize the native Tsp10 protein mainly localized to the stichosome of T. spiralis. Mice immunized with the recombinant phage T7-Tsp10 showed a 62.8% reduction in adult worms and a 78.6% reduction in muscle larvae following challenge with T. spiralis muscle larvae. Our results demonstrate that the vaccination with Tsp10 polypeptide displayed by T7 phage elicits the Th2-predominant immune responses and produces a significant protection against T. spiralis infection in mice. These findings suggest that phage display is a simple, efficient, and promising tool to express candidate vaccine antigens for immunization against T. spiralis.
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China. cuij@zzu.edu.cn
authors.forename: Jing
authors.initials: J
authors.lastname: Cui
authors.forename: Hui Jun
authors.initials: HJ
authors.lastname: Ren
authors.forename: Ruo Dan
authors.initials: RD
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Zi Fang
authors.initials: ZF
authors.lastname: Zhang
authors.forename: Zhong Quan
authors.initials: ZQ
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D017123:Bacteriophage T7 / Q000235:genetics*; D061505:Cell Surface Display Techniques; D004195:Disease Models, Animal; D004337:Drug Carriers; D005260:Female; D005822:Genetic Vectors; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009132:Muscles / Q000469:parasitology; D059208:Parasite Load; D015202:Protein Engineering; D018418:Th2 Cells / Q000276:immunology; D017160:Trichinella spiralis / Q000235:genetics / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology / Q000517:prevention & control*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000947:Antigens, Helminth; D004337:Drug Carriers; D014614:Vaccines, Synthetic