title: Characterization and cellular interaction of fluorescent-labeled PHEMA nanoparticles.
abstract: The aim of this study is to label the cells with polymeric nanoparticles properly and efficiently. For this purpose, acridine orange (AO)-loaded poly(2-hydroxyethyl methacrylate) (PHEMA) nanoparticles were synthesized by miniemulsion polymerization. PHEMA nanoparticles were characterized by zeta sizer, zeta potential, atomic force microscopy (AFM), scanning electron microscopy (SEM), transmission electron microscopy (TEM), fluorescence microscopy, Fourier transform infrared spectrophotometer (FTIR), and elemental analysis. In addition, the toxicity of the nanoparticles were investigated by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay.
authors.affiliation: Department of Nanotechnology and Nanomedicine, Hacettepe University, Ankara-Turkey.
authors.forename: Ilgım
authors.initials: I
authors.lastname: Göktürk
authors.forename: Veyis
authors.initials: V
authors.lastname: Karakoç
authors.forename: Mehmet Ali
authors.initials: MA
authors.lastname: Onur
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D000165:Acridine Orange; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000494:pharmacology; D004655:Emulsions; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D005456:Fluorescent Dyes; D051379:Mice; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D058105:Polymerization; D017550:Spectroscopy, Fourier Transform Infrared; D013194:Staining and Labeling; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D004337:Drug Carriers; D004655:Emulsions; D005456:Fluorescent Dyes; D013778:Tetrazolium Salts; D013844:Thiazoles; D011102:Polyhydroxyethyl Methacrylate; C022616:thiazolyl blue; D000165:Acridine Orange