title: Overcrowding of false codling moth, Thaumatotibia leucotreta (Meyrick) leads to the isolation of five new Cryptophlebia leucotreta granulovirus (CrleGV-SA) isolates.
abstract: False codling moth, Thaumatotibia leucotreta (Meyrick) is a serious pest of economic importance to the South African fruit industry. As part of sustainable efforts to control this pest, biological control options that involve the application of baculovirus-based biopesticides such as Cryptogran and Cryptex (both formulated with a South African isolate of Cryptophlebia leucotreta granulovirus, CrleGV-SA) are popularly used by farmers. In order to safeguard the integrity of these biopesticides as well as protect against any future development of resistance in the host, we conducted a study to bioprospect for additional CrleGV isolates as alternatives to existing ones. Using overcrowding as an induction method for latent infection, we recovered five new CrleGV isolates (CrleGV-SA Ado, CrleGV-SA Mbl, CrleGV-SA Cit, CrleGV-SA MixC and CrleGV-SA Nels). Single restriction endonuclease (REN) analysis of viral genomic DNA extracted from purified occlusion bodies showed that isolates differed in their DNA profiles. Partial sequencing of granulin and egt genes from the different isolates and multiple alignments of nucleotide sequences revealed the presence of single nucleotide polymorphisms (SNPs), some of which resulted in amino acid substitutions in the protein sequence. Based on these findings as well as comparisons with other documented CrleGV isolates, we propose two phylogenetic groups for CrleGV-SA isolates recovered in this study.
authors.affiliation: Department of Zoology and Entomology, PO Box 94, Rhodes University, Grahamstown 6140, South Africa. papajohny@gmail.com
authors.forename: John K
authors.initials: JK
authors.lastname: Opoku-Debrah
authors.forename: Martin P
authors.initials: MP
authors.lastname: Hill
authors.forename: Caroline
authors.initials: C
authors.lastname: Knox
authors.forename: Sean D
authors.initials: SD
authors.lastname: Moore
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000737:chemistry; D060467:Disease Resistance; D025722:Granulovirus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D009036:Moths / Q000502:physiology / Q000821:virology*; D010572:Pest Control, Biological; D010802:Phylogeny; D011156:Population Density; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral