title: Altered matrix mineralization in a case of a sclerosing osteosarcoma.
abstract: Little is known about the tumor matrix mineralization of highly sclerotic osteosarcoma. We used quantitative backscattered electron imaging (qBEI) to determine the Bone mineralization density distribution (BMDD) of a highly sclerosing osteosarcoma of the proximal tibia as well as adjacent normal bone of a 10-year-old girl following chemotherapy according to the EURAMOS-1 protocol. Data were compared to recently published normative reference data for young individuals. Backscattered electron imaging of the tumor region revealed a dense accumulation of mineralized tumor bone matrix (up to 90% of the medullar space). The BMDD was shifted tremendously towards higher matrix mineralization (CaMean +18.5%, CaPeak +22.5%, CaHigh +100 fold) compared to normal bone. Additionally the BMDD became much wider, indicating a higher heterogeneity in mineralization (CaWidth +40%). In contrast to lamellar bone, which mineralizes via a mineralization front, the mineralization of the tumor matrix starts by randomly distributed spots of mineral clusters fusing together to a highly mineralized non-lamellar bone matrix. We also found an altered BMDD of the patient's normal bone when compared with the reference BMDD of young individuals. In conclusion this high radiodensity region of the sclerosing sarcoma is not only due to the high amount of tumor matrix but also to its high mineralization density. Chemotherapy may lead to altered matrix mineralization of normal bone due to suppression of bone turnover. The mechanism of matrix mineralization in a sclerosing osteosarcoma warrants further studies.
authors.affiliation: Department of Orthopaedic Surgery, Vienna General Hospital, Medical University of Vienna, Vienna, Austria. jochen.hofstaetter@meduniwien.ac.at
authors.forename: Jochen G
authors.initials: JG
authors.lastname: Hofstaetter
authors.forename: Andreas
authors.initials: A
authors.lastname: Roschger
authors.forename: Stephan E
authors.initials: SE
authors.lastname: Puchner
authors.forename: Martin
authors.initials: M
authors.lastname: Dominkus
authors.forename: Irene
authors.initials: I
authors.lastname: Sulzbacher
authors.forename: Reinhard
authors.initials: R
authors.lastname: Windhager
authors.forename: Klaus
authors.initials: K
authors.lastname: Klaushofer
authors.forename: Paul
authors.initials: P
authors.lastname: Roschger
mesh_terms: D015519:Bone Density / Q000502:physiology; D002113:Calcification, Physiologic / Q000502:physiology*; D002648:Child; D005260:Female; D006801:Humans; D012516:Osteosarcoma / Q000378:metabolism*