title: miR-143 is critical for the formation of primordial follicles in mice.
abstract: Although microRNAs (miRNAs) have been implicated in fine-tuning gene networks, the roles of mmu-mir-143 (miR-143) in mammalian ovary development have not been studied in vitro. We investigated the expression and function of miR-143 in the mouse ovary during primordial follicle formation. Real-time polymerase chain reaction analysis showed that miR-143 expression increased during primordial follicle formation from 15.5 days post-coitus to 4 days post-partum. miR-143 was located in pregranulosa cells by in situ hybridization. To study the function of miR-143 in primordial follicle formation we established an electroporation transfection model in vitro that allowed miR-143 expression to be efficiently upregulated and inhibited in cultured ovaries. Further studies showed that miR-143 inhibited the formation of primordial follicles by suppressing pregranulosa cell proliferation and downregulating the expression of genes related to the cell cycle. These findings suggest that miR-143 is critical for the formation of primordial follicles and regulates ovarian development and function.
authors.affiliation: State Key Laboratory for Agro-biotechnology, College of Biological Sciences, China Agricultural University, No.2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, P.R. China.
authors.forename: Jianfang
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Ji
authors.forename: Doudou
authors.initials: D
authors.lastname: Zhou
authors.forename: Yueqin
authors.initials: Y
authors.lastname: Li
authors.forename: Junkai
authors.initials: J
authors.lastname: Lin
authors.forename: Jiali
authors.initials: J
authors.lastname: Liu
authors.forename: Haoshu
authors.initials: H
authors.lastname: Luo
authors.forename: Sheng
authors.initials: S
authors.lastname: Cui
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000187:drug effects; D005260:Female; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis / Q000502:physiology*; D006080:Ovarian Follicle / Q000187:drug effects / Q000502:physiology*; D010053:Ovary / Q000254:growth & development
chemical_list: D018797:Cell Cycle Proteins; D035683:MicroRNAs; C532884:MIRN143 microRNA, mouse