title: Oral epithelial cell responses to multispecies microbial biofilms.
abstract: This report describes the use of a novel model of multispecies biofilms to stimulate profiles of cytokines/chemokines from oral epithelial cells that contribute to local inflammation in the periodontium. Streptococcus gordonii (Sg)/S. oralis (So)/S. sanguinis (Ss) and Sg/Fusobacterium nucleatum (Fn)/Porphyromonas gingivalis (Pg) biofilms elicited significantly elevated levels of IL-1α and showed synergistic stimulatory activity compared with an additive effect of the 3 individual bacteria. Only the Sg/Actinomyces naeslundii (An)/Fn multispecies biofilms elicited IL-6 levels above those of control. IL-8 was a primary response to the Sg/An/Fn biofilms, albeit the level was not enhanced compared with a predicted composite level from the monospecies challenges. These results represent some of the first data documenting alterations in profiles of oral epithelial cell responses to multispecies biofilms.
authors.affiliation: Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, KY 40536, USA. rpeyy1@email.uky.edu
authors.forename: R
authors.initials: R
authors.lastname: Peyyala
authors.forename: S S
authors.initials: SS
authors.lastname: Kirakodu
authors.forename: K F
authors.initials: KF
authors.lastname: Novak
authors.forename: J L
authors.initials: JL
authors.lastname: Ebersole
mesh_terms: D018441:Biofilms / Q000254:growth & development; D002461:Cell Line, Transformed; D016207:Cytokines / Q000096:biosynthesis*; D004847:Epithelial Cells / Q000382:microbiology; D016967:Fusobacterium nucleatum / Q000502:physiology; D054884:Host-Pathogen Interactions; D006801:Humans; D053582:Interleukin-1alpha / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D059013:Microbial Consortia / Q000502:physiology*; D009061:Mouth Mucosa / Q000166:cytology / Q000378:metabolism / Q000382:microbiology*; D010518:Periodontitis / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000502:physiology; D013291:Streptococcus / Q000502:physiology
chemical_list: D016207:Cytokines; D053582:Interleukin-1alpha; D015850:Interleukin-6