title: Analysis of cycle gene expression in Aedes aegypti brains by in situ hybridization.
abstract: Even though the blood-sucking mosquito Aedes aegypti is one of the most important disease vectors, relatively little is known about the molecular mechanisms underlying processes involved in the temporal pattern of its activity and host seeking behavior. In this study, we analyzed the expression of the cycle (cyc) gene, one of the core components of the circadian clock, in Ae. aegypti brains by in situ hybridization at two different time points in light-dark conditions and compared the results with those obtained using a quantitative PCR assay (qPCR). Within the brain, differential labeling was detected according to distinct areas empirically pre-defined. Six out of seven of these areas showed significantly higher staining at ZT3 (three hours after light-on) compared to ZT11 (one before light-off), which is consistent with the qPCR data. Predominant staining was observed in three of those areas which correspond to positions of the optical and antennal lobes, as well as the region where the neurons controlling activity rhythms are presumably localized.
authors.affiliation: Laboratório de Biologia Molecular de Insetos, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil.
authors.forename: Samira
authors.initials: S
authors.lastname: Chahad-Ehlers
authors.forename: Carla
authors.initials: C
authors.lastname: Gentile
authors.forename: José Bento Pereira
authors.initials: JB
authors.lastname: Lima
authors.forename: Alexandre Afranio
authors.initials: AA
authors.lastname: Peixoto
authors.forename: Rafaela Vieira
authors.initials: RV
authors.lastname: Bruno
mesh_terms: D056930:ARNTL Transcription Factors / Q000235:genetics / Q000378:metabolism*; D000330:Aedes / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002940:Circadian Rhythm; D018076:DNA, Complementary / Q000378:metabolism; D005786:Gene Expression Regulation; D017403:In Situ Hybridization; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000378:metabolism; D015345:Oligonucleotide Probes / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors
chemical_list: D056930:ARNTL Transcription Factors; D018076:DNA, Complementary; D019476:Insect Proteins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger