title: Microbubble attenuation and destruction: are they involved in sonoporation efficiency?
abstract: This technical note investigates the involvement of microbubble attenuation and destruction in sonoporation mechanisms. First, we evaluate sonoporation efficiency using Vevo Micromarker, and a comparison is made with BR14 and SonoVue microbubbles. Then, the acoustical properties of the microbubbles are measured to gain insight into the sonoporation mechanisms using a green fluorescent protein as a marker. Using glioblastoma cells, an unprecedented transfection rate of 70% is reached with Vevo Micromarker, corresponding to a 1.5-fold increase compared with the rate achieved with the other microbubbles. Moreover, attenuation and destruction were shown to be two key parameters in sonoporation efficiency.
authors.affiliation: UMR Imagerie et Cerveau, Inserm U 930, Universite Francois Rabelais, Tours, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Escoffre
authors.forename: Anthony
authors.initials: A
authors.lastname: Novell
authors.forename: Julien
authors.initials: J
authors.lastname: Piron
authors.forename: Aya
authors.initials: A
authors.lastname: Zeghimi
authors.forename: Alexander
authors.initials: A
authors.lastname: Doinikov
authors.forename: Ayache
authors.initials: A
authors.lastname: Bouakaz
mesh_terms: D045744:Cell Line, Tumor; D003287:Contrast Media / Q000737:chemistry / Q000494:pharmacology; D017930:Genes, Reporter; D005909:Glioblastoma / Q000235:genetics / Q000628:therapy; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D045423:Microbubbles; D013010:Sonication / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors; D014162:Transfection / Q000295:instrumentation / Q000379:methods*; D014465:Ultrasonics
chemical_list: D003287:Contrast Media; D049452:Green Fluorescent Proteins