title: Involvement of amygdaloid protein synthesis in early- and late-phase reconsolidation of emotion-related memories.
abstract: This study was undertaken to test whether de novo protein synthesis in the basolateral (BLA) and central (CeA) nucleus of amygdala was required for reconsolidation of emotion-related memories. Mice were trained to sequentially acquire both cocaine-induced conditioned place preference (CPP) and step through passive avoidance (PA) memories. Immediately following PA retrieval, intra-BLA anisomycin infusion was found to decrease subsequent PA performance in retests. Immediately following PA retrieval, intra-CeA anisomycin infusion did not acutely affect PA performance but decreased such a PA memory 5 days later. Given PA retrieval procedure was omitted, intra-BLA and intra-CeA anisomycin infusion did not affect PA memory. Likewise, intra-BLA anisomycin infusion immediately following cocaine-induced CPP retrieval was found to decrease cocaine-induced CPP magnitude in early and late retests. Immediately after cocaine-induced CPP retrieval, intra-CeA anisomycin infusion did not acutely affect cocaine-induced CPP but decreased this memory 5 days later. Given the cocaine-induced CPP retrieval procedure was omitted, intra-BLA and intra-CeA anisomycin infusion did not affect cocaine-induced CPP in subsequent retests. These results, taken together, imply that de novo protein synthesis in amygdala plays an important role in modulating reconsolidation of emotion-related memory. More importantly, de novo protein synthesis in the BLA is essential for early phase reconsolidation of retrieved emotion-related memories. Protein synthesis in the CeA is required for late phase reconsolidation of retrieved emotion-related memories.
authors.affiliation: National Cheng Kung University College of Medicine, Tainan, Taiwan, Republic of China.
authors.forename: Jia-Ying
authors.initials: JY
authors.lastname: Chuang
authors.forename: Kuey-Yin
authors.initials: KY
authors.lastname: Lin
authors.forename: Chianfang G
authors.initials: CG
authors.lastname: Cherng
authors.forename: Lung
authors.initials: L
authors.lastname: Yu
mesh_terms: D000679:Amygdala / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D000841:Anisomycin / Q000494:pharmacology; D001362:Avoidance Learning / Q000187:drug effects / Q000502:physiology*; D003042:Cocaine / Q000494:pharmacology; D003213:Conditioning, Psychological / Q000187:drug effects / Q000502:physiology; D018765:Dopamine Uptake Inhibitors / Q000494:pharmacology; D004644:Emotions / Q000502:physiology*; D008297:Male; D011939:Mental Recall / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008845:Microinjections / Q000379:methods; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology
chemical_list: D018765:Dopamine Uptake Inhibitors; D009419:Nerve Tissue Proteins; D011500:Protein Synthesis Inhibitors; D000841:Anisomycin; D003042:Cocaine