title: A synonymous mutation in NOD2 gene was significantly associated with non-specific digestive disorder in rabbit.
abstract: Nucleotide-binding oligomerization domain containing 2 (NOD2) plays a pivotal role in the host innate and adaptive immunity by recognizing the pathogenic agents. Therefore, its genetic polymorphisms and association with susceptibility to infectious diseases have been widely reported in human and farm animals. In the present study, we investigated the genetic polymorphisms in 3171 bp coding region of NOD2 gene and association with non-specific digestive disorder (NSDD) in rabbit. A total of four coding single-nucleotide polymorphisms (cSNPs) were detected. Among them, c.2961C>T was further genotyped for case (n=176) and control (n=130) based on association analysis, which revealed that C allele carried the potential protective role for susceptibility to NSDD with the odds ratio (OR) values of 0.52 (95% confidence interval (CI) 0.37-0.73, P<0.01). Under the dominant inheritance model, CC genotype was associated with decreased susceptibility to NSDD (OR=0.38, 95% CI 0.24-0.60, P<0.01). Along with the aggravation of NSDD, we observed higher mRNA expression of NOD2 gene (P<0.05). However, the mRNA expression pattern of CC genotype would be interacted by the different status of NSDD, which only showed the significantly increased level in severe NSDD group (P<0.05). These results revealed by genetic association and gene expression analysis suggested that the NOD2 gene was associated with the susceptibility to NSDD in rabbit. However, the causative mutations linked to c.2961C>T and corresponding functional depiction should be further explored by performing exhaustive genetic studies.
authors.affiliation: Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Chengdu Campus, Chengdu 611130, China.
authors.forename: Wen-Xiu
authors.initials: WX
authors.lastname: Zhang
authors.forename: Gong-Wei
authors.initials: GW
authors.lastname: Zhang
authors.forename: Jin
authors.initials: J
authors.lastname: Peng
authors.forename: Juan-Li
authors.initials: JL
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.forename: Song-Jia
authors.initials: SJ
authors.lastname: Lai
mesh_terms: D000818:Animals; D016022:Case-Control Studies; D004252:DNA Mutational Analysis; D004066:Digestive System Diseases / Q000235:genetics* / Q000662:veterinary*; D056726:Genetic Association Studies / Q000662:veterinary; D020022:Genetic Predisposition to Disease; D005838:Genotype; D053473:Nod2 Signaling Adaptor Protein / Q000235:genetics* / Q000378:metabolism; D017354:Point Mutation / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits
chemical_list: D053473:Nod2 Signaling Adaptor Protein; D012333:RNA, Messenger