title: The molecular clock regulates circadian transcription of tissue factor gene.
abstract: Tissue factor (TF) is involved in endotoxin-induced inflammation and mortality. We found that the circadian expression of TF mRNA, which peaked at the day to night transition (activity onset), was damped in the liver of Clock mutant mice. Luciferase reporter and chromatin immunoprecipitation analyses using embryonic fibroblasts derived from wild-type or Clock mutant mice showed that CLOCK is involved in transcription of the TF gene. Furthermore, the results of real-time luciferase reporter experiments revealed that the circadian expression of TF mRNA is regulated by clock molecules through a cell-autonomous mechanism via an E-box element located in the promoter region.
authors.affiliation: Biological Clock Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan. k-ooishi@aist.go.jp
authors.forename: Katsutaka
authors.initials: K
authors.lastname: Oishi
authors.forename: Satoru
authors.initials: S
authors.lastname: Koyanagi
authors.forename: Naoki
authors.initials: N
authors.lastname: Ohkura
mesh_terms: D000818:Animals; D056926:CLOCK Proteins / Q000235:genetics / Q000378:metabolism*; D002940:Circadian Rhythm / Q000235:genetics*; D024721:E-Box Elements; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D013925:Thromboplastin / Q000235:genetics*; D015534:Trans-Activators; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D015534:Trans-Activators; D013925:Thromboplastin; D056926:CLOCK Proteins; C538944:Clock protein, mouse