title: Decreased microRNA-30a levels are associated with enhanced ABL1 and BCR-ABL1 expression in chronic myeloid leukemia.
abstract: Chronic myeloid leukemia (CML) is associated with overexpression of BCR-ABL1, a nonreceptor tyrosine kinase critical for malignant transformation. We investigated whether non-coding microRNAs (miRNAs) targeting BCR-ABL1 mRNA contribute to the pathogenesis of CML. Indeed, miR-30a targeted BCR-ABL1 and was underexpressed in bone marrow from CML patients. In K562 leukemia cells, overexpression of miR-30a reduced ABL1 and BCR-ABL1 protein expression, decreased proliferation, and arrested cell cycle progression between G1 and S. These findings strongly suggest that miR-30a acts as a tumor suppressor by downregulating ABL1 and BCR-ABL1 expression. Upregulation of miR-30a in hematopoietic cells may have therapeutic efficacy against CML.
authors.affiliation: Institute of Genetic Engineering, Southern Medical University, Guangzhou, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Liu
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Song
authors.forename: Wenli
authors.initials: W
authors.lastname: Ma
authors.forename: Wenling
authors.initials: W
authors.lastname: Zheng
authors.forename: Hong
authors.initials: H
authors.lastname: Yin
mesh_terms: D016022:Case-Control Studies; D002478:Cells, Cultured; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D016147:Genes, Tumor Suppressor / Q000502:physiology; D016313:Genes, abl / Q000235:genetics*; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D034741:RNA, Small Interfering / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
chemical_list: C546086:MIRN30b microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D016044:Fusion Proteins, bcr-abl