title: Reprogramming to pluripotency and differentiation of cells with synthetic mRNA.
abstract: Changing cell fate without altering its genome is very desirable in many experimental systems and for cell therapy. Compared to DNA plasmid or viral-based approach, mRNA has the advantage of high transfection efficiency, no danger of changing the genome or creating mutational insertions. Here, we describe a straightforward protocol to synthesize mRNA of genes of interest, and use them to induce pluripotency and direct cell differentiation.
authors.affiliation: School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Peizhe
authors.initials: P
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Na
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D060846:Cell Engineering / Q000379:methods*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014162:Transfection / Q000379:methods*
chemical_list: D012333:RNA, Messenger