title: Monitoring oncogenic B-RAF-induced senescence in melanocytes.
abstract: The B-RAF kinase is a downstream effector of the RAS family of proto-oncogenes and is constitutively activated in the majority of human melanomas. The common oncogenic B-RAF(V600E) mutant cooperates with additional genetic lesions to transform immortal murine and human cells. In primary cells, however, B-RAF(V600E) triggers a rapid cell cycle arrest that is phenotypically indistinguishable from cellular senescence. Here we describe the analyses of B-RAF-induced senescence in primary human melanocytes using recombinant lentiviruses.
authors.affiliation: Melanoma Institute Australia, North Sydney, Australia.
authors.forename: Sieu L
authors.initials: SL
authors.lastname: Tran
authors.forename: Helen
authors.initials: H
authors.lastname: Rizos
mesh_terms: D001973:Bromodeoxyuridine / Q000378:metabolism; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D016922:Cellular Senescence / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008544:Melanocytes / Q000166:cytology* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009154:Mutation; D009857:Oncogenes / Q000235:genetics*; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D012867:Skin / Q000166:cytology; D013194:Staining and Labeling; D014161:Transduction, Genetic; D019076:Transgenes / Q000235:genetics; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D048493:Proto-Oncogene Proteins B-raf; D001616:beta-Galactosidase; D001973:Bromodeoxyuridine