title: Anti-inflammatory Lactobacillus rhamnosus CNCM I-3690 strain protects against oxidative stress and increases lifespan in Caenorhabditis elegans.
abstract: Numerous studies have shown that resistance to oxidative stress is crucial to stay healthy and to reduce the adverse effects of aging. Accordingly, nutritional interventions using antioxidant food-grade compounds or food products are currently an interesting option to help improve health and quality of life in the elderly. Live lactic acid bacteria (LAB) administered in food, such as probiotics, may be good antioxidant candidates. Nevertheless, information about LAB-induced oxidative stress protection is scarce. To identify and characterize new potential antioxidant probiotic strains, we have developed a new functional screening method using the nematode Caenorhabditis elegans as host. C. elegans were fed on different LAB strains (78 in total) and nematode viability was assessed after oxidative stress (3 mM and 5 mM H(2)O(2)). One strain, identified as Lactobacillus rhamnosus CNCM I-3690, protected worms by increasing their viability by 30% and, also, increased average worm lifespan by 20%. Moreover, transcriptomic analysis of C. elegans fed with this strain showed that increased lifespan is correlated with differential expression of the DAF-16/insulin-like pathway, which is highly conserved in humans. This strain also had a clear anti-inflammatory profile when co-cultured with HT-29 cells, stimulated by pro-inflammatory cytokines, and co-culture systems with HT-29 cells and DC in the presence of LPS. Finally, this Lactobacillus strain reduced inflammation in a murine model of colitis. This work suggests that C. elegans is a fast, predictive and convenient screening tool to identify new potential antioxidant probiotic strains for subsequent use in humans.
authors.affiliation: Danone Research, Gut Microbiology & Probiotics Platform, Palaiseau, France. gianfranco.grompone@danone.com
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Grompone
authors.forename: Patricia
authors.initials: P
authors.lastname: Martorell
authors.forename: Silvia
authors.initials: S
authors.lastname: Llopis
authors.forename: Núria
authors.initials: N
authors.lastname: González
authors.forename: Salvador
authors.initials: S
authors.lastname: Genovés
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Mulet
authors.forename: Tamara
authors.initials: T
authors.lastname: Fernández-Calero
authors.forename: Inés
authors.initials: I
authors.lastname: Tiscornia
authors.forename: Mariela
authors.initials: M
authors.lastname: Bollati-Fogolín
authors.forename: Isabelle
authors.initials: I
authors.lastname: Chambaud
authors.forename: Benoit
authors.initials: B
authors.lastname: Foligné
authors.forename: Agustín
authors.initials: A
authors.lastname: Montserrat
authors.forename: Daniel
authors.initials: D
authors.lastname: Ramón
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism / Q000382:microbiology* / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism / Q000382:microbiology; D005260:Female; D051858:Forkhead Transcription Factors; D020869:Gene Expression Profiling; D019073:HT29 Cells; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000382:microbiology; D007328:Insulin / Q000378:metabolism; D007778:Lactobacillus / Q000502:physiology*; D008136:Longevity; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D019936:Probiotics / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D011972:Receptor, Insulin / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013045:Species Specificity; D014157:Transcription Factors / Q000378:metabolism; D014302:Trinitrobenzenesulfonic Acid / Q000009:adverse effects
chemical_list: D029742:Caenorhabditis elegans Proteins; D051858:Forkhead Transcription Factors; D007328:Insulin; D017382:Reactive Oxygen Species; D014157:Transcription Factors; C108831:daf-16 protein, C elegans; D014302:Trinitrobenzenesulfonic Acid; C107483:DAF-2 protein, C elegans; D011972:Receptor, Insulin