title: 2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts.
abstract: There is growing demand for robust DNA assembly strategies to quickly and accurately fabricate genetic circuits for synthetic biology. One application of this technology is reconstitution of multi-gene assemblies. Here, we integrate a new software tool chain with 2ab assembly and show that it is robust enough to generate 528 distinct composite parts with an error-free success rate of 96%. Finally, we discuss our findings in the context of its implications for biosafety and biosecurity.
authors.affiliation: Department of Bioengineering, University of California, Berkeley, CA, 94720, USA. jcanderson@berkeley.edu.
authors.forename: Mariana
authors.initials: M
authors.lastname: Leguia
authors.forename: Jennifer An
authors.initials: JA
authors.lastname: Brophy
authors.forename: Douglas
authors.initials: D
authors.lastname: Densmore
authors.forename: Angel
authors.initials: A
authors.lastname: Asante
authors.forename: J Christopher
authors.initials: JC
authors.lastname: Anderson