title: [Experimental study of suspicious sensitization component screening on Huachansu injection].
abstract: OBJECTIVE
  To explore a new experimental method for screening of allergens in post-market traditional Chinese medicine injections by confirming allergens in Huachansu injection.
  
  
  METHODS
  First of all, the serum of patients allergic to Huachansu injection were collected, at the same time, the dubious allergen was conjugated to bovine serum albumin (BSA) by EDC coupling procedure to form complete antigen (BNP-BSA), which makes it possible to reproduce the allergic reaction of Huachansu injection in vitro. The histamine liberation ratio, the level of TNF-alpha and Histamine released from RBL-2H3 mast cell were detected; the above data were compared with those obtained in vivo.
  
  
  RESULTS
  The difference of the histamine liberation ratio, the levels of TNF-alpha and histamine of the resibufogenin-BSA group, group of patients allergic to Huachansu injection were not significant compared with those of normal control group. However, there were significant difference in those data among the cinobufagin-BSA group, the blank control and normal control group (P<0.05).
  
  
  CONCLUSIONS
  The allergen in the serum collected from patients allergic to Huachansu injection is resibufogenin.
authors.affiliation: Shijiazhuang College, Shijiazhuang 050035, China. yingxiao629@163.com
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiao
authors.forename: Yu-Bin
authors.initials: YB
authors.lastname: Zhao
authors.forename: Yan-Ming
authors.initials: YM
authors.lastname: Xie
mesh_terms: D000485:Allergens / Q000009:adverse effects / Q000276:immunology*; D000664:Amphibian Venoms / Q000009:adverse effects / Q000276:immunology*; D000818:Animals; D001001:Anura; D002018:Bufanolides / Q000009:adverse effects / Q000276:immunology; D004342:Drug Hypersensitivity / Q000276:immunology*; D006636:Histamine Release; D006801:Humans; D008407:Mast Cells / Q000276:immunology; D008516:Medicine, Chinese Traditional
chemical_list: D000485:Allergens; D000664:Amphibian Venoms; D002018:Bufanolides; C545813:huachansu; C002471:cinobufagin