title: Growth-phase dependent variation in photosynthetic activity and cellular protein expression profile in the harmful raphidophyte Chattonella antiqua.
abstract: This study investigated temporal variations in the potential maximum quantum yield of photosystem II (F(v)/F(m) ratio) and growth-phase dependent cellular protein expressions of Chattonella antiqua under laboratory conditions. Despite the culture conditions, significant positive correlations between the F(v)/F(m) ratio and daily growth rate were observed. Threshold F(v)/F(m) ratios associated with positive cell growth were calculated to be >0.44, >0.44, and >0.37, and those associated with active cell growth (growth rate >0.5 div. d(-1)) were >0.58, >0.60, and >0.49 under control culture, low nutrient and intense light conditions, respectively. Proteome profiles obtained by two-dimensional gel electrophoresis (2-DE) indicated that 42 protein spots were differentially expressed at various growth phases of C. antiqua, which indicates changes in cellular physiological status throughout the growth cycle, and suggests that oxygen evolving enhancer 1 and 2-cysteine peroxiredoxin play roles in maintaining the positive growth of C. antiqua.
authors.affiliation: Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
authors.forename: Xuchun
authors.initials: X
authors.lastname: Qiu
authors.forename: Yohei
authors.initials: Y
authors.lastname: Shimasaki
authors.forename: Michito
authors.initials: M
authors.lastname: Tsuyama
authors.forename: Taku
authors.initials: T
authors.lastname: Yamada
authors.forename: Rie
authors.initials: R
authors.lastname: Kuwahara
authors.forename: Mayumi
authors.initials: M
authors.lastname: Kawaguchi
authors.forename: Masato
authors.initials: M
authors.lastname: Honda
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Gunjikake
authors.forename: Rumana
authors.initials: R
authors.lastname: Tasmin
authors.forename: Motoyuki
authors.initials: M
authors.lastname: Shimizu
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sato
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kato-Unoki
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakashima
authors.forename: Tadashi
authors.initials: T
authors.lastname: Matsubara
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yamasaki
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Ichinose
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Wariishi
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Honjo
authors.forename: Yuji
authors.initials: Y
authors.lastname: Oshima
mesh_terms: D020418:Algal Proteins / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D002734:Chlorophyll / Q000502:physiology; D015180:Electrophoresis, Gel, Two-Dimensional; D015870:Gene Expression; D020869:Gene Expression Profiling; D006863:Hydrogen-Ion Concentration; D008027:Light; D008969:Molecular Sequence Data; D054464:Peroxiredoxins / Q000235:genetics / Q000378:metabolism*; D010788:Photosynthesis / Q000235:genetics*; D045332:Photosystem II Protein Complex / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D058009:Stramenopiles / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism
chemical_list: D020418:Algal Proteins; D045332:Photosystem II Protein Complex; D002734:Chlorophyll; D054464:Peroxiredoxins