title: Experimental design methodologies in the optimization of chiral CE or CEC separations: an overview.
abstract: In this chapter, an overview of experimental designs to develop chiral capillary electrophoresis (CE) and capillary electrochromatographic (CEC) methods is presented. Method development is generally divided into technique selection, method optimization, and method validation. In the method optimization part, often two phases can be distinguished, i.e., a screening and an optimization phase. In method validation, the method is evaluated on its fit for purpose. A validation item, also applying experimental designs, is robustness testing. In the screening phase and in robustness testing, screening designs are applied. During the optimization phase, response surface designs are used. The different design types and their application steps are discussed in this chapter and illustrated by examples of chiral CE and CEC methods.
authors.affiliation: Department of Analytical Chemistry and Pharmaceutical Technology, Vrije Universiteit Brussel, Brussels, Belgium.
authors.forename: Bieke
authors.initials: B
authors.lastname: Dejaegher
authors.forename: Debby
authors.initials: D
authors.lastname: Mangelings
authors.forename: Yvan
authors.initials: Y
authors.lastname: Vander Heyden
mesh_terms: D053801:Capillary Electrochromatography / Q000379:methods*; D003505:Cyclodextrins / Q000032:analysis / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D012044:Regression Analysis; D015203:Reproducibility of Results; D012107:Research Design; D013237:Stereoisomerism
chemical_list: D003505:Cyclodextrins