title: Immunomodulatory effect of a proanthocyanidin-rich extract from Pinus radiata bark by dosing period in chickens.
abstract: We evaluated the immunomodulatory effects of a proanthocyanidin-rich extract (PAE) from Pinus radiata bark in Korean native chickens. In experiment 1, proliferation of peripheral blood mononuclear cells was significantly enhanced in chickens administered 1.25, 2.5, 5, and 10 mg/kg of PAE for 2 wk once daily by oral gavage compared with no administration of PAE in vitro. Proliferation of splenocyte and thymocyte cells was also significantly enhanced in chickens administered 1.25, 2.5, 5, and 10 mg/kg of PAE for 5 wk. In experiment 2, proliferation of peripheral blood mononuclear cells, splenocytes, and thymocytes in chickens administered PAE for 6 wk was continuously higher than that in other groups. Additionally, increasing cell populations for which CD4(+)CD8(-)(Th cells) and Bu-1(+) (B cells) protein was expressed on the cell surface were significantly different after 4 wk of PAE administration in chickens. Thus, the immunomodulatory effects of a PAE from P. radiata bark differed by dosing period in chickens.
authors.affiliation: Departments of Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Korea Zoonosis Research Institute, Chonbuk National University, Chonbuk, 561-756, Korea.
authors.forename: In-Jae
authors.initials: IJ
authors.lastname: Park
authors.forename: Se-Yeoun
authors.initials: SY
authors.lastname: Cha
authors.forename: Min
authors.initials: M
authors.lastname: Kang
authors.forename: Hyung-Kwan
authors.initials: HK
authors.lastname: Jang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002060:Bursa of Fabricius / Q000166:cytology / Q000187:drug effects; D049109:Cell Proliferation; D002645:Chickens / Q000276:immunology*; D003208:Concanavalin A / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D007723:Korea; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D008070:Lipopolysaccharides / Q000737:chemistry; D028223:Pinus / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D044945:Proanthocyanidins / Q000494:pharmacology*; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects
chemical_list: D008070:Lipopolysaccharides; D010936:Plant Extracts; D044945:Proanthocyanidins; D003208:Concanavalin A