title: Exo70 subunit of the exocyst complex is involved in adhesion-dependent trafficking of caveolin-1.
abstract: Caveolae are specialized domains of the plasma membrane, which play key roles in signaling, endocytosis and mechanosensing. Using total internal reflection fluorescent microscopy (TIRF-M), we observe that the exocyst subunit Exo70 forms punctuate structures at the plasma membrane and partially localizes with caveolin-1, the main component of caveolae. Upon cell detachment, we found that Exo70 accumulates with caveolin-1-positive vesicular structures. Upon cell re-adhesion, caveolin-1 traffics back to the plasma membrane in a multistep process involving microtubules and actin cytoskeleton. In addition, silencing of Exo70 redirects caveolin-1 to focal adhesions identified by markers such as α5 integrin or vinculin. Based on these findings, we conclude that Exo70 is involved in caveolin-1 recycling to the plasma membrane during re-adhesion of the cells to the substratum.
authors.affiliation: Membrane and Cytoskeleton Dynamics, Institut Curie, Research Center, CNRS- UMR144, Paris, France. maud.hertzog@ibcg.biotoul.fr
authors.forename: Maud
authors.initials: M
authors.lastname: Hertzog
authors.forename: Pedro
authors.initials: P
authors.lastname: Monteiro
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Le Dez
authors.forename: Philippe
authors.initials: P
authors.lastname: Chavrier
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure; D021941:Caveolae / Q000378:metabolism; D051242:Caveolin 1 / Q000378:metabolism*; D002448:Cell Adhesion; D022001:Focal Adhesions / Q000378:metabolism; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D021122:Protein Subunits / Q000378:metabolism; D021381:Protein Transport; D034741:RNA, Small Interfering / Q000235:genetics; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C495540:CAV1 protein, human; D051242:Caveolin 1; C547754:EXOC7 protein, human; D021122:Protein Subunits; D034741:RNA, Small Interfering; D033921:Vesicular Transport Proteins