title: Daily sperm production: application in studies of prenatal exposure to nanoparticles in mice.
abstract: We investigated the influence of maternal airway exposure to nanoparticulate titanium dioxide (TiO₂, UV-Titan) and carbon black (CB, Printex90), on male reproductive function in the two following generations. Time-mated C57BL/6J mice were exposed by inhalation to UV-Titan, or by intratracheal instillation with Printex90. Body and testicle weight, sperm content per g testicular parenchyma and daily sperm production (DSP) were assessed. The protocol for assessment of DSP was optimized for application in mice (C57BL/6J) and the influence of different parameters was studied. Maternal particulate exposure did not affect DSP statistically significantly in the F1 generation, although TiO₂ tended to reduce sperm counts. Overall, time-to-first F2 litter increased with decreasing sperm production. There was no effect on sperm production in the F2 generation originating after TiO₂ exposure. F2 offspring, whose fathers were prenatally exposed to Printex90, showed lowered sperm production. Furthermore, we report statistically significant differences in sperm production between mouse strains.
authors.affiliation: National Research Centre for the Working Environment, Lersø Parkallé 105, DK-2100 Copenhagen Ø, Denmark. zky@nrcwe.dk.
authors.forename: Zdenka Orabi
authors.initials: ZO
authors.lastname: Kyjovska
authors.forename: Anne Mette Z
authors.initials: AM
authors.lastname: Boisen
authors.forename: Petra
authors.initials: P
authors.lastname: Jackson
authors.forename: Håkan
authors.initials: H
authors.lastname: Wallin
authors.forename: Ulla
authors.initials: U
authors.lastname: Vogel
authors.forename: Karin Sørig
authors.initials: KS
authors.lastname: Hougaard
mesh_terms: D000818:Animals; D004351:Drug Resistance; D005260:Female; D006824:Hybridization, Genetic; D007248:Infertility, Male / Q000139:chemically induced* / Q000209:etiology / Q000503:physiopathology; D019570:Inhalation Exposure / Q000009:adverse effects*; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects*; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000633:toxicity*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009929:Organ Size / Q000187:drug effects; D018812:Paternal Exposure / Q000009:adverse effects; D011247:Pregnancy; D053260:Soot / Q000008:administration & dosage / Q000633:toxicity*; D013045:Species Specificity; D013091:Spermatogenesis / Q000187:drug effects*; D013737:Testis / Q000187:drug effects / Q000473:pathology; D014025:Titanium / Q000008:administration & dosage / Q000633:toxicity*; D018675:Toxicity Tests; D015430:Weight Gain / Q000187:drug effects
chemical_list: D053260:Soot; C009495:titanium dioxide; D014025:Titanium