title: Streptococcus orisasini sp. nov. and Streptococcus dentasini sp. nov., isolated from the oral cavity of donkeys.
abstract: Four Gram-positive, catalase-negative, coccoid isolates that were obtained from donkey oral cavities formed two distinct clonal groups when characterized by phenotypic and phylogenetic studies. From the results of biochemical tests, the organisms were tentatively identified as a streptococcal species. Comparative 16S rRNA gene sequencing studies confirmed the organisms to be members of the genus Streptococcus. Two of the isolates were related most closely to Streptococcus ursoris with 95.6 % similarity based on the 16S rRNA gene and to Streptococcus ratti with 92.0 % similarity based on the 60 kDa heat-shock protein gene (groEL). The other two isolates, however, were related to Streptococcus criceti with 95.0 and 89.0 % similarities based on the 16S rRNA and groEL genes, respectively. From both phylogenetic and phenotypic evidence, the four isolates formed two distinct clonal groups and are suggested to represent novel species of the genus Streptococcus. The names proposed for these organisms are Streptococcus orisasini sp. nov. (type strain NUM 1801(T) = JCM 17942(T) = DSM 25193(T)) and Streptococcus dentasini sp. nov. (type strain NUM 1808(T) = JCM 17943(T) = DSM 25137(T)).
authors.affiliation: Department of Oral Microbiology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan.
authors.forename: Kazuko
authors.initials: K
authors.lastname: Takada
authors.affiliation: Department of Oral Microbiology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Saito
authors.affiliation: Laboratory Medicine for Dentistry, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Tsudukibashi
authors.affiliation: Department of Genome Medicine, Allergy and Immunology Project, Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo 156-8506, Japan.
authors.forename: Takachika
authors.initials: T
authors.lastname: Hiroi
authors.affiliation: Department of Oral Microbiology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan.
authors.forename: Masatomo
authors.initials: M
authors.lastname: Hirasawa
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D018834:Chaperonin 60 / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D018397:Equidae / Q000382:microbiology*; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009055:Mouth / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013291:Streptococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D018834:Chaperonin 60; D004269:DNA, Bacterial; D006360:Heat-Shock Proteins; D012336:RNA, Ribosomal, 16S