title: [Humoral immune response in patients with angina and soft tissue infections of group a streptococci etiology].
abstract: Evaluate informativity of simultaneous determination of antibodies (AB) against extracellular (AB against streptolysin-O-ASL-O) and cellular (IgM against A-polysaccharide - A-PSC) antigens in patients with angina and soft tissue infections caused by serogroup A streptococci (SGA) and identify features of humoral immune response to SGA infection according to infectious process localization. MATERIALS AND METHODS. 2 groups of patients with bacteriologically confirmed SGA infection (50 cases of angina - group 1 and 51 case of soft tissue infection - group 2) were examined for the presence of ASL-O by using Architect ci8200 analyzer (Abbott, USA) and IgM against SGA A-PSC by EIA. RESULTS. In group 1, 23 (46%) individuals were recognized as positive by ASL-O level, and in group 2 - 20 (39%; p>0.05); conditionally significant exceeding of normal values (more than 1.5 times) was detected in 25% of patients of each group. Increased level of antibodies against SGA A-PSC was detected in 43 (86%) patients of group 1, and in 30 (59%) of patients of group 2 (p<0.05). In group 1 exceeding of normal values of anti-A-PSC IgM was noted mostly by 1.5 +/- 0.5 times (74%). In group 2 in 43% of patients the level of anti-A-PSC IgM was above normal more than 2 times and in most cases in uncomplicated variants of disease course. In 45% of patients with severe form of soft tissue infection this parameter did not exceed normal values (p<0.05). CONCLUSION. In acute period of disease with simultaneous determination of ASL-O and IgM against A-PSC sensitivity of serologic diagnostics of SGA etiology angina and SGA infection of soft tissues was established to reach 92% and 72%, respectively, and humoral immune response to cellular AG in each form of SGA has its features.
authors.forename: D A
authors.initials: DA
authors.lastname: Kleĭmenov
authors.forename: E V
authors.initials: EV
authors.lastname: Glushkova
authors.forename: N F
authors.initials: NF
authors.lastname: Dmitrieva
authors.forename: A S
authors.initials: AS
authors.lastname: Eshchina
authors.forename: Iu M
authors.initials: IuM
authors.lastname: Timofeev
authors.forename: N A
authors.initials: NA
authors.lastname: Malyshev
authors.forename: N I
authors.initials: NI
authors.lastname: Briko
mesh_terms: D000328:Adult; D000368:Aged; D000888:Antibodies, Anti-Idiotypic / Q000097:blood* / Q000276:immunology; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D001426:Bacterial Proteins / Q000097:blood / Q000276:immunology; D005260:Female; D006801:Humans; D056724:Immunity, Humoral; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D018461:Soft Tissue Infections / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D013290:Streptococcal Infections / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D013297:Streptococcus pyogenes / Q000276:immunology*; D013301:Streptolysins / Q000097:blood* / Q000276:immunology
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D013301:Streptolysins; C044203:anti-IgM; C025964:streptolysin O