title: Yeast Gis2 and its human ortholog CNBP are novel components of stress-induced RNP granules.
abstract: Although a CCTG expansion in the gene encoding the zinc knuckle protein CNBP causes a common form of muscular dystrophy, the function of both human CNBP and its putative budding yeast ortholog Gis2 remain poorly understood. Here we report the protein interactions of Gis2 and the subcellular locations of both Gis2 and CNBP. We found that Gis2 exhibits RNA-dependent interactions with two proteins involved in mRNA recognition, the poly(A) binding protein and the translation initiation factor eIF4G. We show that Gis2 is a component of two large RNA-protein granules, processing bodies and stress granules, which contain translationally repressed mRNAs. Consistent with a functional ortholog, CNBP also associates with the poly(A) binding protein and accumulates in stress granules during arsenite treatment of human cells. These results implicate both Gis2 and CNBP in mRNA handling during stress.
authors.affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut, USA.
authors.forename: Marta
authors.initials: M
authors.lastname: Rojas
authors.forename: George W
authors.initials: GW
authors.lastname: Farr
authors.forename: Cesar F
authors.initials: CF
authors.lastname: Fernandez
authors.forename: Laura
authors.initials: L
authors.lastname: Lauden
authors.forename: John C
authors.initials: JC
authors.lastname: McCormack
authors.forename: Sandra L
authors.initials: SL
authors.lastname: Wolin
mesh_terms: D003594:Cytoplasmic Granules / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D011132:Polyribosomes / Q000378:metabolism; D011485:Protein Binding; D014176:Protein Biosynthesis; D020871:RNA Stability; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: C433227:CNBP protein, human; C557900:Gis2 protein, S cerevisiae; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D029701:Saccharomyces cerevisiae Proteins