title: Detection of Aeromonas hydrophila in liquid media by volatile production similarity patterns, using a FF-2A electronic nose.
abstract: A technique for rapid detection of pathogenic microorganisms is essential for the diagnosis of associated infections and for food safety analysis. Aeromonas hydrophila is one such food contaminant. Several methods for rapid detection of this pathogen have been developed; these include multiplex polymerase chain reaction assays and the colony overlay procedure for peptidases. However, these conventional methods can only be used to detect the microorganisms at high accuracy after symptomatic onset of the disease. Therefore, in the future, simple pre-screening methods may be useful for preventing food poisoning and disease. In this paper, we present a novel system for the rapid detection of the microorganism A. hydrophila in cultured media (in <2 h), with the use of an electronic nose (FF-2A). With this electronic nose, we detected the changes of volatile patterns produced by A. hydrophila after 30 min culture. Our calculations revealed that the increased volatiles were similar to the odours of organic acids and esters. In future, distinctive volatile production patterns of microorganisms identified with the electronic nose may have the potential in microorganism detection.
authors.affiliation: Department of Molecular Cell Biology, Institute of DNA Medicine, Research Center for Medical Sciences, The Jikei University School of Medicine, Tokyo, Japan. kfujioka@jikei.ac.jp
authors.forename: Kouki
authors.initials: K
authors.lastname: Fujioka
authors.forename: Eiji
authors.initials: E
authors.lastname: Arakawa
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Kita
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Aoyama
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Manome
authors.forename: Keiichi
authors.initials: K
authors.lastname: Ikeda
authors.forename: Kenji
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D016980:Aeromonas hydrophila / Q000254:growth & development / Q000302:isolation & purification*; D000465:Algorithms; D003470:Culture Media / Q000737:chemistry*; D062609:Electronic Nose; D005740:Gases / Q000378:metabolism; D013997:Time Factors; D014835:Volatilization
chemical_list: D003470:Culture Media; D005740:Gases