title: Systematic identification of rhythmic genes reveals camk1gb as a new element in the circadian clockwork.
abstract: A wide variety of biochemical, physiological, and molecular processes are known to have daily rhythms driven by an endogenous circadian clock. While extensive research has greatly improved our understanding of the molecular mechanisms that constitute the circadian clock, the links between this clock and dependent processes have remained elusive. To address this gap in our knowledge, we have used RNA sequencing (RNA-seq) and DNA microarrays to systematically identify clock-controlled genes in the zebrafish pineal gland. In addition to a comprehensive view of the expression pattern of known clock components within this master clock tissue, this approach has revealed novel potential elements of the circadian timing system. We have implicated one rhythmically expressed gene, camk1gb, in connecting the clock with downstream physiology of the pineal gland. Remarkably, knockdown of camk1gb disrupts locomotor activity in the whole larva, even though it is predominantly expressed within the pineal gland. Therefore, it appears that camk1gb plays a role in linking the pineal master clock with the periphery.
authors.affiliation: Department of Neurobiology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Adi
authors.initials: A
authors.lastname: Tovin
authors.forename: Shahar
authors.initials: S
authors.lastname: Alon
authors.forename: Zohar
authors.initials: Z
authors.lastname: Ben-Moshe
authors.forename: Philipp
authors.initials: P
authors.lastname: Mracek
authors.forename: Gad
authors.initials: G
authors.lastname: Vatine
authors.forename: Nicholas S
authors.initials: NS
authors.lastname: Foulkes
authors.forename: Jasmine
authors.initials: J
authors.lastname: Jacob-Hirsch
authors.forename: Gideon
authors.initials: G
authors.lastname: Rechavi
authors.forename: Reiko
authors.initials: R
authors.lastname: Toyama
authors.forename: Steven L
authors.initials: SL
authors.lastname: Coon
authors.forename: David C
authors.initials: DC
authors.lastname: Klein
authors.forename: Eli
authors.initials: E
authors.lastname: Eisenberg
authors.forename: Yoav
authors.initials: Y
authors.lastname: Gothilf
mesh_terms: D000818:Animals; D057906:Circadian Clocks / Q000235:genetics / Q000502:physiology; D002940:Circadian Rhythm / Q000235:genetics* / Q000502:physiology; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D007814:Larva / Q000235:genetics / Q000254:growth & development; D020411:Oligonucleotide Array Sequence Analysis; D010870:Pineal Gland / Q000254:growth & development / Q000378:metabolism / Q000502:physiology; D017423:Sequence Analysis, RNA; D015027:Zebrafish / Q000235:genetics / Q000254:growth & development; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology
chemical_list: D029961:Zebrafish Proteins