title: The value of molecular expression of KIT and KIT ligand analysed using real-time polymerase chain reaction and immunohistochemistry as a prognostic indicator for canine cutaneous mast cell tumours.
abstract: This study investigated the correlation between KIT gene expression determined by immunohistochemistry and real-time polymerase chain reaction (RT-PCR) and the rate of tumour recurrence and tumour-related deaths in dogs affected with mast cell tumour (MCT). Kaplan-Meier curves were constructed to compare tumour recurrence and tumour-related death between patients. The log-rank test was used to check for significant differences between curves. KIT-I, KIT-II and KIT-III staining patterns were observed in 9 (11.11%), 50 (61.73%) and 22 (27.16%) tumours, respectively. Tumour recurrence rates and tumour-related deaths were not associated with KIT staining patterns (P = 0278, P > 0.05), KIT (P = 0.289, P > 0.05) or KIT ligand (P = 0.106, P > 0.05) gene expression. Despite the lack of association between KIT staining pattern and patient survival time, the results suggest a correlation between aberrant KIT localization and increased proliferative activity of MCTs. RT-PCR seems to be a sensible method for quantitative detection of KIT gene expression in canine MCT, although expressions levels are not correlated with prognosis.
authors.affiliation: Biotechnology Master Program, Positivo University, Curitiba, Paraná, Brazil.
authors.forename: T A
authors.initials: TA
authors.lastname: Costa Casagrande
authors.forename: L M
authors.initials: LM
authors.lastname: de Oliveira Barros
authors.forename: H
authors.initials: H
authors.lastname: Fukumasu
authors.forename: B
authors.initials: B
authors.lastname: Cogliati
authors.forename: L M
authors.initials: LM
authors.lastname: Chaible
authors.forename: M L Z
authors.initials: ML
authors.lastname: Dagli
authors.forename: J M
authors.initials: JM
authors.lastname: Matera
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor; D004283:Dog Diseases / Q000378:metabolism* / Q000473:pathology; D004285:Dogs; D015972:Gene Expression Regulation, Neoplastic; D007150:Immunohistochemistry / Q000662:veterinary*; D034801:Mastocytoma / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary*; D012878:Skin Neoplasms / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D019089:Stem Cell Factor / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D019089:Stem Cell Factor; D019009:Proto-Oncogene Proteins c-kit
keywords: c-kit; immunohistochemistry; mastocitoma; survival time