title: Interactions of bone marrow stromal cells with native and RGD surface modified acellular bone matrix: a biocompatibility study.
abstract: Acellular bone matrix (ACM) is a promising biodegradable material scaffold. However, poor cell attachment properties of ACM limit its application. This study investigated the biocompatibility of mesenchymal stem cells (MSCs) and native or surface modified ACM (RGD-ACM) scaffolds in the construction of tissue-engineered bone. Wistar rat bone marrow-derived MSCs were isolated by density gradient centrifugation for in vitro culture. MSCs were seeded on ACM with or without surface modification by RGD peptides to generate tissue-engineered bone for in vitro culture. Significantly higher adhesion of MSCs to RGD-ACM was observed compared with the native material. Detection of active alkaline phosphatase (ALP) indicated intercalation of this protein into the scaffold enhanced cell proliferation. Complete distribution and adhesion of MSCs to the surface of the scaffold was observed by scanning electron microscope. ACM with surface modification by RGD peptides improved the seeding adhesion rates and facilitated the growth efficiency of tissue-engineered bone.
authors.affiliation: Department of Orthopedics, General Hospital of Tianjin Medical University, Tianjin Hospital, China.
authors.forename: Hua-feng
authors.initials: HF
authors.lastname: Zhang
authors.forename: Zhi-jun
authors.initials: ZJ
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Fu
authors.forename: Jian-xiong
authors.initials: JX
authors.lastname: Ma
authors.forename: Xin-long
authors.initials: XL
authors.lastname: Ma
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology; D001857:Bone Matrix / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry; D055114:X-Ray Microtomography
chemical_list: D001672:Biocompatible Materials; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D000469:Alkaline Phosphatase