title: Siderophore mediated uranium sequestration by marine cyanobacterium Synechococcus elongatus BDU 130911.
abstract: Four different marine cyanobacterial morphotypes were tested for their efficacy to produce siderophores in Fe minus [Fe(-)], Fe minus Uranium dosed [Fe(-)U(+)], and Fe dosed Uranium dosed [Fe(-)U(+)] media. Of the four organisms tested, Synechococcus elongatus BDU 130911 produced the highest amount of siderophore of 58μgmg(-1) dryweight. The results clearly indicate that uranium induces siderophore production in marine cyanobacteria even in the presence of iron [Fe(-)U(+)] condition. The type of siderophore revealed by FeCl(3), Tetrazolium and Atkin's tests is a hydroxamate; and thin layer chromatogram also authenticates our finding. Uranium siderophore complexation was confirmed through modified Chrome Azurol S (CAS) assay as well as based on residual uranium presence. In silico docking studies further validate siderophore complexation with uranium.
authors.affiliation: National Facility for Marine Cyanobacteria (Sponsored by DBT, Govt. of India), Department of Marine Biotechnology, School of Marine Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
authors.forename: Vijayaraghavan
authors.initials: V
authors.lastname: Rashmi
authors.forename: Mohandass
authors.initials: M
authors.lastname: Shylajanaciyar
authors.forename: Ramamoorthy
authors.initials: R
authors.lastname: Rajalakshmi
authors.forename: Stanley F
authors.initials: SF
authors.lastname: D'Souza
authors.forename: Dharmar
authors.initials: D
authors.lastname: Prabaharan
authors.forename: Lakshmanan
authors.initials: L
authors.lastname: Uma
mesh_terms: D062105:Molecular Docking Simulation; D017262:Siderophores / Q000096:biosynthesis*; D046940:Synechococcus / Q000254:growth & development / Q000378:metabolism*; D014501:Uranium / Q000378:metabolism*
chemical_list: D017262:Siderophores; D014501:Uranium