title: [Steady-state spectroscopy on complementary tissue structural and functional information extraction].
abstract: Steady-state spectroscopy is prevailing for complementary tissue structural and functional information extraction The goal of the present study is to experimentally validate the merit of this technique for optical properties and intrinsic fluorescence extraction from tissue mimicking phantoms. Phantom studies were carried out in which HbO2 was used as absorber, polystyrene sphere was used as scatterer, and stilbene was used for fluorescence emission Two sets of phantoms corresponding to two absorption levels and one scattering level were made, and 4 fluorophore concentrations were titrated in each set. The results indicate that both optical properties and intrinsic fluorescence can be extracted with high accuracy, implying that it's feasible to extract complementary structural and functional information with steady-state spectroscopy.
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, and Institute of Biomedical Analytical Technology and Instrumentation, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China. andycbliu@gmail.com
authors.forename: Cheng-Bo
authors.initials: CB
authors.lastname: Liu
authors.forename: Jing-Bo
authors.initials: JB
authors.lastname: Song
authors.forename: Tao
authors.initials: T
authors.lastname: Long
authors.forename: Zhen-Xi
authors.initials: ZX
authors.lastname: Zhang
mesh_terms: D000465:Algorithms; D003198:Computer Simulation; D006454:Hemoglobins / Q000737:chemistry*; D009010:Monte Carlo Method; D011137:Polystyrenes / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D013057:Spectrum Analysis / Q000379:methods*; D013267:Stilbenes / Q000737:chemistry
chemical_list: D006454:Hemoglobins; D011137:Polystyrenes; D013267:Stilbenes