title: In-solution digestion of glycoproteins for glycopeptide-based mass analysis.
abstract: Glycopeptides are generated from the enzymatic digestion of glycoproteins with a specific or nonspecific protease. Whether this enzymatic conversion of glycoproteins into glycopeptides and peptides is done in-solution or in-gel, an efficient digestion protocol is one of the key components of a successful outcome in a mass spectrometry-based experimental workflow. This chapter outlines an optimized in-solution digestion protocol to prepare samples for glycopeptide-based mass analysis.
authors.affiliation: Department of Chemistry, University of Kansas, Lawrence, KS, USA.
authors.forename: Eden P
authors.initials: EP
authors.lastname: Go
authors.forename: Kathryn R
authors.initials: KR
authors.lastname: Rebecchi
authors.forename: Heather
authors.initials: H
authors.lastname: Desaire
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D006020:Glycopeptides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D010447:Peptide Hydrolases / Q000378:metabolism; D059748:Proteolysis; D012996:Solutions; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D006020:Glycopeptides; D006023:Glycoproteins; D012996:Solutions; D010447:Peptide Hydrolases