title: Vitamin D treatment modulates organic dust-induced cellular and airway inflammatory consequences.
abstract: Exposure to organic dusts elicits airway inflammatory diseases. Vitamin D recently has been associated with various airway inflammatory diseases, but its role in agricultural organic dust exposures is unknown. This study investigated whether vitamin D reduces organic dust-induced inflammatory outcomes in cell culture and animal models. Organic dust extracts obtained from swine confinement facilities induced neutrophil chemokine production (human IL-8, murine CXCL1/CXCL2). Neutrophil chemokine induction was reduced in human blood monocytes, human bronchial epithelial cells, and murine lung slices pretreated with 1,25-(OH)(2) D(3) . Intranasal inhalation of organic dust extract induced neutrophil influx, and CXCL1/CXCL2 release was also decreased in mice fed a relatively high vitamin D diet as compared to mice fed a low vitamin D diet. These findings were associated with reduced tracheal epithelial cell PKCα and PKCε activity and whole lung TLR2 and TLR4 gene expression. Collectively, vitamin D plays a role in modulating organic dust-induced airway inflammatory outcomes.
authors.affiliation: Pulmonary, Critical Care, Sleep and Allergy Division, Department of Medicine, University of Nebraska Medical Center, Omaha, NE 68198-5300, USA.
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Golden
authors.forename: Todd A
authors.initials: TA
authors.lastname: Wyatt
authors.forename: Debra J
authors.initials: DJ
authors.lastname: Romberger
authors.forename: Daniel
authors.initials: D
authors.lastname: Reiff
authors.forename: Michael
authors.initials: M
authors.lastname: McCaskill
authors.forename: Christopher
authors.initials: C
authors.lastname: Bauer
authors.forename: Angela M
authors.initials: AM
authors.lastname: Gleason
authors.forename: Jill A
authors.initials: JA
authors.lastname: Poole
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D002460:Cell Line; D054360:Chemokine CXCL1 / Q000378:metabolism; D054426:Chemokine CXCL2 / Q000378:metabolism; D018925:Chemokines / Q000378:metabolism*; D004195:Disease Models, Animal; D004391:Dust; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation / Q000188:drug therapy* / Q000209:etiology* / Q000276:immunology; D016209:Interleukin-8 / Q000378:metabolism; D008168:Lung / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000187:drug effects; D058871:Organic Agriculture; D011493:Protein Kinase C / Q000378:metabolism; D013552:Swine; D051195:Toll-Like Receptor 2 / Q000235:genetics; D051197:Toll-Like Receptor 4 / Q000235:genetics; D014807:Vitamin D / Q000494:pharmacology*
chemical_list: D054360:Chemokine CXCL1; D054426:Chemokine CXCL2; D018925:Chemokines; C475895:Cxcl1 protein, mouse; C404915:Cxcl2 protein, mouse; D004391:Dust; D016209:Interleukin-8; C495342:TLR2 protein, human; C495345:TLR4 protein, human; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D014807:Vitamin D; D011493:Protein Kinase C