title: Construction of a marker system for the evaluation of competitiveness for legume nodulation in Rhizobium strains.
abstract: A marker system has been set up for the analysis of competitiveness of Rhizobium leguminosarum strains for legume nodulation. The strains generated incorporate gusA and celB marker genes at identical positions and allow efficient scoring of single and double infections. Based on this system, we have found that strain UPM791 outcompetes 3841 for nodulation in pea. This technique will be useful to determine the effect of different traits on competitiveness.
authors.affiliation: Centro de Biotecnología y Genómica de Plantas (C.B.G.P.), and Departamento de Biotecnología, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Campus de Montegancedo, Carretera M40-km 37.7, 28223 Pozuelo de Alarcón, Madrid, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Sánchez-Cañizares
authors.forename: Jose
authors.initials: J
authors.lastname: Palacios
mesh_terms: D002480:Cellulase / Q000235:genetics / Q000378:metabolism; D060085:Coinfection; D017930:Genes, Reporter; D005959:Glucosidases / Q000235:genetics / Q000378:metabolism; D018532:Pisum sativum / Q000382:microbiology* / Q000502:physiology*; D055170:Plant Root Nodulation; D016961:Rhizobium leguminosarum / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D053204:Root Nodules, Plant / Q000382:microbiology*; D013194:Staining and Labeling
chemical_list: C109965:CelB endoglucanase; D005959:Glucosidases; D002480:Cellulase