title: [Lactobacillus plantarum NDC 75017 affects il-6 gene expression in Caco-2 cells].
abstract: OBJECTIVE
  We tested the expression of immune-related gene interleukin 6 (il-6) in vitro to understand the influence from Lactobacillus plantarum NDC 75017 on host cells and further to reveal the regulatory mechanism.
  
  
  METHODS
  Caco-2 cells were cocultured with L. plantarum NDC 75017 for 0, 2, 4, 6, 8, 10 and 12 h, the total RNA were extracted; then the expressions of il-6 and tlr2 genes were analyzed by Real Time RT-PCR. The phosphorylation level of NF-KB was analyzed by Western Blot after the Caco-2 cells stimulation with L. plantarum NDC 75017 at 0, 0.5, 1, 2 and 4 h. Caco-2 cells were pretreated with pyrrolidine dithiocarbamate for 30 min before being treated with L. plantarum NDC 75017 for 2 h, then the total RNA was extracted and the expressions of il-6 and tlr2 genes were analyzed by Real Time RT-PCR.
  
  
  RESULTS
  Lactobacillus plantarum NDC 75017 could induce the expressions of il-6 and tlr2 in Caco-2 cells, the il-6 and tlr2 expressions peaked at 8 h and 6 h after cocultured with L. plantarum NDC 75017. L. plantarum NDC 75017 could rapidly activate the phosphorylation of NF-kappaB, and the expressions of il-6 and tlr2 were decreased notably after pretreated with pyrrolidine dithiocarbamate.
  
  
  CONCLUSIONS
  L. plantarum NDC 75017 could up-regulate and then down-regulate the expression of il6 through rapidly activating tlr2-mediated NF-kappaB signaling pathway in Caco-2 cells.
authors.affiliation: Key Lab of Dairy Science, Ministry of Education, College of Food Science and Engineering, Northeast Agricultural University, Harbin 150030, China. 549979215@qq.com
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Chaoxin
authors.initials: C
authors.lastname: Man
authors.forename: Xuena
authors.initials: X
authors.lastname: Lv
authors.forename: Linlin
authors.initials: L
authors.lastname: Han
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Xue
authors.forename: Xiangyi
authors.initials: X
authors.lastname: Yang
authors.forename: Yi
authors.initials: Y
authors.lastname: Shan
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yujun
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D018938:Caco-2 Cells; D005786:Gene Expression Regulation; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics* / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000382:microbiology*; D048191:Lactiplantibacillus plantarum / Q000235:genetics / Q000502:physiology*; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism
chemical_list: D015850:Interleukin-6; C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2