title: [Senescent endothelial dysfunctions were mediated by S1P2 receptor in cultured human umbilical vein endothelial cells].
abstract: OBJECTIVE
  To investigate the variation of senescent endothelial function by regulating the sphingosine-1-phosphate receptor type 2 (S1P2) expression in cultured human umbilical vein endothelial cells (HUVECs).
  
  
  METHODS
  The S1P2 receptor expression was regulated by transfecting the cDNA or shRNA of S1P2 in cultured HUVECs. The expression levels of S1P2 receptor in HUVECs were detected by RT-PCR and Western blot. EC chemotaxis was measured by the transwell migration assay. The wound healing assay was performed by a scratch wound model on EC monolayer. Matrigel morphogenesis assay was employed to assess the in vitro angiogenic responses.
  
  
  RESULTS
  After up-regulating the S1P2 expression in young ECs, the S1P-stimulated formation of a tubular-like network in Matrigel was dramatically diminished in transfected ECs (P<0.05). Quantification of the wound healing assay showed that transfected ECs grew much slower than young ECs (P<0.05). The chemotactic capability was significantly decreased in transfected ECs (P<0.05). Furthermore, the senescent-associated impairments were revoked by the downregulation of S1P2 receptor in senescent HUVECs.
  
  
  CONCLUSIONS
  The impaired functions (chemotactic, wound-healing and morphogenetic responses) in senescent HUVECs in vitro are mediated by S1P2 receptor.
authors.affiliation: Department of Biochemistry, Central South University, Changsha, China.
authors.forename: Shuhua
authors.initials: S
authors.lastname: Chen
authors.forename: Hong
authors.initials: H
authors.lastname: Xiang
authors.forename: Guoping
authors.initials: G
authors.lastname: Yang
authors.forename: Hao
authors.initials: H
authors.lastname: Deng
authors.forename: Hong
authors.initials: H
authors.lastname: Yuan
authors.forename: Hongwei
authors.initials: H
authors.lastname: Lu
mesh_terms: D002478:Cells, Cultured; D016922:Cellular Senescence / Q000235:genetics*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D049349:Receptors, Lysosphingolipid / Q000235:genetics / Q000378:metabolism*; D000081025:Sphingosine-1-Phosphate Receptors; D014162:Transfection; D015854:Up-Regulation
chemical_list: D034741:RNA, Small Interfering; D049349:Receptors, Lysosphingolipid; C477522:S1PR2 protein, human; D000081025:Sphingosine-1-Phosphate Receptors