title: GsSRK, a G-type lectin S-receptor-like serine/threonine protein kinase, is a positive regulator of plant tolerance to salt stress.
abstract: Receptor-like protein kinases (RLKs) play vital roles in sensing outside signals, yet little is known about RLKs functions and roles in stress signal perception and transduction in plants, especially in wild soybean. Through the microarray analysis, GsSRK was identified as an alkaline (NaHCO3)-responsive gene, and was subsequently isolated from Glycine soja by homologous cloning. GsSRK encodes a 93.22kDa protein with a highly conserved serine/threonine protein kinase catalytic domain, a G-type lectin region, and an S-locus region. Real-time PCR results showed that the expression levels of GsSRK were largely induced by ABA, salt, and drought stresses. Over expression of GsSRK in Arabidopsis promoted seed germination, as well as primary root and rosette leaf growth during the early stages of salt stress. Compared to the wild type Arabidopsis, GsSRK overexpressors exhibited enhanced salt tolerance and higher yields under salt stress, with higher chlorophyll content, lower ion leakage, higher plant height, and more siliques at the adult developmental stage. Our studies suggest that GsSRK plays a crucial role in plant response to salt stress.
authors.affiliation: Plant Bioengineering Laboratory, Northeast Agricultural University, Harbin 150030, China. sunxiaoli2008@yahoo.cn
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Sun
authors.forename: Qing-Yue
authors.initials: QY
authors.lastname: Yu
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Tang
authors.forename: Wei
authors.initials: W
authors.lastname: Ji
authors.forename: Xi
authors.initials: X
authors.lastname: Bai
authors.forename: Hua
authors.initials: H
authors.lastname: Cai
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Liu
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Ding
authors.forename: Yan-Ming
authors.initials: YM
authors.lastname: Zhu
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000222:Adaptation, Physiological / Q000187:drug effects* / Q000235:genetics; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018525:Germination / Q000187:drug effects / Q000235:genetics; D037102:Lectins / Q000378:metabolism*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D054712:Salinity; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D020539:Sequence Analysis, Protein; D012965:Sodium Chloride / Q000494:pharmacology*; D013025:Glycine max / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D013312:Stress, Physiological / Q000187:drug effects* / Q000235:genetics; D013997:Time Factors
chemical_list: D037102:Lectins; D010940:Plant Proteins; D012333:RNA, Messenger; D012965:Sodium Chloride; D000040:Abscisic Acid; D017346:Protein Serine-Threonine Kinases