title: Differential alkaloid profile in Uncaria tomentosa micropropagated plantlets and root cultures.
abstract: The alkaloids of Uncaria tomentosa micropropagated plantlets and root cultures were isolated and identified by NMR and mass spectrometry. Plantlets yielded pteropodine (1), isopteropodine (2), mitraphylline (3), isomitraphylline (4), uncarine F (5), speciophylline (6), rhynchophylline (7) and isorhynchophylline (8). In plantlets growing under continuous light, tetracyclic alkaloids 7 and 8 decreased from 20 ± 1.8 at 2 months to 2.2 ± 0.33 mg/g dry wt at 6 months, while the pentacyclic alkaloids 1-4 increased from 7.7 ± 1.4 to 15 ± 0.05 mg/g dry wt, supporting their biogenetic conversion. Micropropagated plantlets produced four times more alkaloids (27.6 ± 3.1 mg/g dry wt) than greenhouse plants. Plantlet roots yielded 3, 4, 8 and the glucoindole alkaloids 3α-dihydrocadambine (9) and dolichantoside (10), the last one not previously found in Uncaria.
authors.affiliation: Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av. I. P. N. 2508. Col. San Pedro Zacatenco, 07360 Mexico, DF, Mexico.
authors.forename: Gabriela R
authors.initials: GR
authors.lastname: Luna-Palencia
authors.forename: Ariana A
authors.initials: AA
authors.lastname: Huerta-Heredia
authors.forename: Carlos M
authors.initials: CM
authors.lastname: Cerda-García-Rojas
authors.forename: Ana C
authors.initials: AC
authors.lastname: Ramos-Valdivia
mesh_terms: D000470:Alkaloids / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D020948:Cat's Claw / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D007211:Indoles / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D008958:Models, Molecular; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D046509:Tissue Culture Techniques
chemical_list: D000470:Alkaloids; D007211:Indoles; D010936:Plant Extracts