title: Inhibition of hyaluronidase by N-acetyl cysteine and glutathione: role of thiol group in hyaluronan protection.
abstract: Hyaluronidase inhibitors have immense applications in pathophysiological conditions associated with hyaluronan-hyaluronidase system. The present study demonstrates the inhibitory efficacy of clinically accepted antioxidant N-acetyl cysteine (NAC) against hyaluronidase of serum, testis, and snake and bee venoms. The experimental and molecular dynamic simulation data suggest the non-competitive inhibition and involvement of thiol groups of both NAC and glutathione in exertion of inhibition. The bioavailability, less-toxic and antioxidant nature of NAC and glutathione could become valuable in the management of pathologies triggered by extracellular matrix degradation and to increase the endurance of hyaluronan based biomaterials/supplements, which are highly exciting aspects.
authors.affiliation: Department of Studies in Biochemistry, University of Mysore, Manasagangothri, Mysore 570 006, Karnataka, India.
authors.forename: K
authors.initials: K
authors.lastname: Sunitha
authors.forename: P
authors.initials: P
authors.lastname: Suresh
authors.forename: M Sebastin
authors.initials: MS
authors.lastname: Santhosh
authors.forename: M
authors.initials: M
authors.lastname: Hemshekhar
authors.forename: R M
authors.initials: RM
authors.lastname: Thushara
authors.forename: Gopal K
authors.initials: GK
authors.lastname: Marathe
authors.forename: C
authors.initials: C
authors.lastname: Thirunavukkarasu
authors.forename: K
authors.initials: K
authors.lastname: Kemparaju
authors.forename: M Suresh
authors.initials: MS
authors.lastname: Kumar
authors.forename: K S
authors.initials: KS
authors.lastname: Girish
mesh_terms: D000111:Acetylcysteine / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D005978:Glutathione / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D008968:Molecular Conformation; D062105:Molecular Docking Simulation; D056004:Molecular Dynamics Simulation; D011485:Protein Binding
chemical_list: D004791:Enzyme Inhibitors; D006821:Hyaluronoglucosaminidase; D005978:Glutathione; D000111:Acetylcysteine