title: Antibodies against Yariv's reagent for immunolocalization of arabinogalactan-proteins in aerial parts of Echinacea purpurea.
abstract: Arabinogalactan-proteins are glycoproteins that occur in higher plants and are involved in important processes like cell differentiation and plant growth. In the medicinal plant Echinacea purpurea L., they belong to the putative immunomodulating compounds and are structurally well characterized. For microscopic localization of arabinogalactan-proteins, synthetic (β-D-Glc)3 Yariv phenylglycoside that specifically binds to most plant arabinogalactan-proteins was used to label arabinogalactan-proteins in fresh cut sections of stems and petioles of Echinacea purpurea. Polyclonal antibodies against (β-D-Glc)3 Yariv phenylglycoside were used to detect the arabinogalactan-protein-(β-D-Glc)3 Yariv phenylglycoside complex. After addition of fluorescein isothiocyanate-conjugated secondary antibodies, the sections were analyzed by confocal laser scanning microscopy. Arabinogalactan-proteins are localized mainly in the central cylinder in the collateral vascular bundles, especially in the area of the xylem. In cell walls of fully differentiated vessels and tracheids, arabinogalactan-proteins have been detected mainly at the inner area of the wall close to the cell lumina. Intense labeling occurs around pit canals connecting adjacent vessels. Furthermore, arabinogalactan-proteins are present in the lumina of cells of the sclerenchyma caps and in companion cells of the phloem.
authors.affiliation: Pharmaceutical Institute, Department of Pharmaceutical Biology, Christian-Albrechts-University of Kiel, Kiel, Germany. mgoellner@pharmazie.uni-kiel.de
authors.forename: Esther Marie
authors.initials: EM
authors.lastname: Göllner
authors.forename: Jean Christian
authors.initials: JC
authors.lastname: Gramann
authors.forename: Birgit
authors.initials: B
authors.lastname: Classen
mesh_terms: D000906:Antibodies / Q000276:immunology; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D020900:Echinacea / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D005960:Glucosides / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism; D007202:Indicators and Reagents; D018613:Microscopy, Confocal; D009088:Mucoproteins / Q000276:immunology* / Q000378:metabolism; D010696:Phloroglucinol / Q000031:analogs & derivatives* / Q000276:immunology; D010940:Plant Proteins / Q000276:immunology / Q000378:metabolism; D018547:Plant Stems / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D058526:Plant Vascular Bundle / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D010946:Plants, Medicinal; D012680:Sensitivity and Specificity; D013194:Staining and Labeling
chemical_list: D000906:Antibodies; D005960:Glucosides; D006023:Glycoproteins; D007202:Indicators and Reagents; D009088:Mucoproteins; D010940:Plant Proteins; C104977:Yariv reagent; C006619:arabinogalactan proteins; D010696:Phloroglucinol