title: Glycated collagen and altered glucose increase endothelial cell adhesion strength.
abstract: Cell adhesion strength is important to cell survival, proliferation, migration, and mechanotransduction, yet changes in endothelial cell adhesion strength have not yet been examined in diseases such as diabetes with high rates of cardiovascular complications. We therefore investigated porcine aortic endothelial cell adhesion strength on native and glycated collagen-coated substrates and in low, normal, and high glucose culture using a spinning disc apparatus. Adhesion strength increased by 30 dynes/cm(2) in cells on glycated collagen as compared to native collagen. Attachment studies revealed that cells use higher adhesion strength αv β3 integrins to bind to glycated collagen instead of the typical α2 β1 integrins used to bind to native collagen. Similarly, endothelial cells cultured in low and high glucose had 15 dynes/cm(2) higher adhesion strength than cells in normal glucose after 2 days. Increased adhesion strength was due to elevated VEGF release and intracellular PKC in low and high glucose cells, respectively. Thus glucose increased endothelial cell adhesion strength via different underlying mechanisms. These adhesion strength changes could contribute to diabetic vascular disease, including accelerated atherosclerosis and disordered angiogenesis.
authors.affiliation: Department of Mechanical Engineering and Mechanics, Drexel University, Philadelphia, Pennsylvania, USA.
authors.forename: Steven Frank
authors.initials: SF
authors.lastname: Kemeny
authors.forename: Stephanie
authors.initials: S
authors.lastname: Cicalese
authors.forename: Dannielle Solomon
authors.initials: DS
authors.lastname: Figueroa
authors.forename: Alisa Morss
authors.initials: AM
authors.lastname: Clyne
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D003094:Collagen / Q000378:metabolism*; D003925:Diabetic Angiopathies / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D004730:Endothelium, Vascular / Q000166:cytology* / Q000473:pathology / Q000502:physiology*; D005947:Glucose / Q000737:chemistry / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D013379:Substrate Specificity / Q000502:physiology; D013552:Swine; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D020099:Coated Materials, Biocompatible; D006023:Glycoproteins; D042461:Vascular Endothelial Growth Factor A; D003094:Collagen; D005947:Glucose