title: Effects of fasting and feeding on the brain mRNA expressions of orexin, tyrosine hydroxylase (TH), PYY and CCK in the Mexican blind cavefish (Astyanax fasciatus mexicanus).
abstract: The effects of fasting and feeding on the brain expression of orexin (OX), tyrosine hydroxylase (TH), peptide Y (PY) and cholecystokinin (CCK) were examined in the blind cavefish Astyanax fasciatus mexicanus. A 10-days fasting period induced increases in both OX and TH brain mRNA expression but had no effect on PYY and CCK expression. Periprandial changes in expression were seen for OX, TH and PYY but not for CCK. OX brain expression peaked 1h prior to a scheduled meal and decreased 1h post feeding in fed fish. A peak in TH expression was seen 1h post feeding in unfed fish whereas a peak in PYY expression was seen 1h post feeding in fed fish. Our result indicates that brain OX, TH and PYY might be involved in the central regulation of feeding of blind cavefish.
authors.affiliation: Department of Biology, Memorial University of Newfoundland, St. John's, NL, Canada.
authors.forename: Alicia
authors.initials: A
authors.lastname: Wall
authors.forename: Hélène
authors.initials: H
authors.lastname: Volkoff
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism*; D060126:Characidae / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D002766:Cholecystokinin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D005215:Fasting; D005247:Feeding Behavior; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000068797:Orexins; D019894:Peptide YY / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment; D014446:Tyrosine 3-Monooxygenase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D029941:Fish Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009479:Neuropeptides; D000068797:Orexins; D012333:RNA, Messenger; D019894:Peptide YY; D002766:Cholecystokinin; D014446:Tyrosine 3-Monooxygenase