title: [Examination of cytotoxic effect of anti-cancer drug doxorubicin on human embryonic stem cells].
abstract: Cytotoxic effect of anti-cancer drug, doxorubicin (DR), has been examined on human embryonic stem cells (ESC) C910 and fibroblasts spontaneously differentiated from these cells. The fibroblasts retained diploid karyotype. It was found that ESC are more sensitive to DR than fibroblasts: DR dose killing 20% cells was 0.01 and 0.1 microg/ml, respectively. DR induced ESC apoptotic death and reduced both ESC and fibroblast proliferation. Unlike fibroblasts DR reversibly inhibited ESC proliferation. Thus, we have demonstrated that ESC and their differentiated derivates differ their sensitivity and response to the genotoxic agent.
authors.forename: I V
authors.initials: IV
authors.lastname: Kozhukharova
authors.forename: T M
authors.initials: TM
authors.lastname: Grinchuk
authors.forename: N A
authors.initials: NA
authors.lastname: Pugovkina
authors.forename: Z V
authors.initials: ZV
authors.lastname: Kovaleva
authors.forename: L P
authors.initials: LP
authors.lastname: Alekseenko
authors.forename: N N
authors.initials: NN
authors.lastname: Nikol'skiĭ
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D003603:Cytotoxins / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology*; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans
chemical_list: D000903:Antibiotics, Antineoplastic; D003603:Cytotoxins; D004317:Doxorubicin