title: Effect of labeling with iron oxide particles or nanodiamonds on the functionality of adipose-derived mesenchymal stem cells.
abstract: Stem cells are increasingly the focus of translational research as well as having emerging roles in human cellular therapy. To support these uses there is a need for improved methods for in vivo cell localization and tracking. In this study, we examined the effects of cell labeling on the in vitro functionality of human adipose-derived mesenchymal stem cells. Our results provide a basis for future in vivo studies investigating implanted cell fate and longevity. In particular, we investigated the effects of two different particles: micron-sized (~0.9 µm) fluorescently labeled (Dragon Green) superparamagnetic iron oxide particles (M-SPIO particles); and, carboxylated nanodiamonds of ~0.25 µm in size. The effects of labeling on the functionality of adipose-derived MSCs were assessed by in vitro morphology, osteogenic and adipogenic differentiation potential, CD marker expression, cytokine secretion profiling and quantitative proteomics of the intra-cellular proteome. The differentiation and CD marker assays for stem-like functionality were not altered upon label incorporation and no secreted or intra-cellular protein changes indicative of stress or toxicity were detected. These in vitro results indicate that the M-SPIO particles and nanodiamonds investigated in this study are biocompatible with MSCs and therefore would be suitable labels for cell localization and tracking in vivo.
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, North Ryde, New South Wales, Australia.
authors.forename: Sinead P
authors.initials: SP
authors.lastname: Blaber
authors.forename: Cameron J
authors.initials: CJ
authors.lastname: Hill
authors.forename: Rebecca A
authors.initials: RA
authors.lastname: Webster
authors.forename: Jana M
authors.initials: JM
authors.lastname: Say
authors.forename: Louise J
authors.initials: LJ
authors.lastname: Brown
authors.forename: Shih-Chang
authors.initials: SC
authors.lastname: Wang
authors.forename: Graham
authors.initials: G
authors.lastname: Vesey
authors.forename: Benjamin Ross
authors.initials: BR
authors.lastname: Herbert
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D001672:Biocompatible Materials / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D002448:Cell Adhesion; D019070:Cell Lineage; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival; D004396:Coloring Agents / Q000494:pharmacology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D003911:Dextrans / Q000494:pharmacology; D018130:Diamond / Q000737:chemistry*; D005290:Ferric Compounds / Q000494:pharmacology*; D006801:Humans; D015187:Lipectomy; D008280:Magnetics; D058185:Magnetite Nanoparticles; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D053758:Nanoparticles / Q000737:chemistry; D040901:Proteomics / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D004396:Coloring Agents; D017077:Culture Media, Conditioned; D003911:Dextrans; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C000499:ferric oxide; D002244:Carbon; D018130:Diamond; C065507:ferumoxides