title: Exercise-induced oxidative stress in elderly subjects: the effect of red orange supplementation on the biochemical and cellular response to a single bout of intense physical activity.
abstract: Aging is characterized by an impaired capacity to maintain the redox balance both in physiological and pathological situations associated with an increased production of reactive oxygen species. Since the extent of this phenomenon may be influenced by an antioxidants-rich diet, we investigated the effect of supplementation with fresh red orange juice (ROJ) on biochemical and cellular biomarkers of oxidative stress in healthy, trained elderly women after a single bout of exhaustive exercise (EE). To this purpose, a sample of 22 females, 15 (69.0 ± 5.1 years) taking the ROJ supplementation and 7 (68.1 ± 2.7 years) as Control group, was constituted. Blood samples were collected immediately before, 30 minutes, and 24 hr after a single bout of EE, at baseline and after 4 weeks. Our results demonstrate that markers of DNA damage or apoptosis were not affected by EE both in Control and ROJ group, and by ROJ, whereas, exercise temporarily affected the redox balance in both groups. Controls didn't change their response to EE after the experimental period, but experimental group after ROJ supplementation had lower EE-induced MDA, consumed less ascorbic acid, and had less activation of the hypoxanthine/xanthine system, i.e., they seemed to be protected from hypoxia/reoxygenation mechanisms.
authors.affiliation: Integrated Laboratory of Biology and Biochemistry of Human Movement, Department of Health Sciences, University of Rome Foro Italico,- IUSM, Rome, Italy. monica.pittaluga@uniroma4.it
authors.forename: M
authors.initials: M
authors.lastname: Pittaluga
authors.forename: A
authors.initials: A
authors.lastname: Sgadari
authors.forename: B
authors.initials: B
authors.lastname: Tavazzi
authors.forename: C
authors.initials: C
authors.lastname: Fantini
authors.forename: S
authors.initials: S
authors.lastname: Sabatini
authors.forename: R
authors.initials: R
authors.lastname: Ceci
authors.forename: A M
authors.initials: AM
authors.lastname: Amorini
authors.forename: P
authors.initials: P
authors.lastname: Parisi
authors.forename: D
authors.initials: D
authors.lastname: Caporossi
mesh_terms: D000284:Administration, Oral; D000368:Aged; D000975:Antioxidants / Q000008:administration & dosage*; D001205:Ascorbic Acid / Q000097:blood; D001628:Beverages; D015415:Biomarkers / Q000097:blood; D032084:Citrus sinensis / Q000737:chemistry*; D004249:DNA Damage; D019587:Dietary Supplements; D015444:Exercise; D005260:Female; D006461:Hemolysis; D006706:Homeostasis / Q000187:drug effects; D006801:Humans; D019271:Hypoxanthine / Q000097:blood; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D005082:Physical Exertion; D010936:Plant Extracts / Q000008:administration & dosage*; D019820:Xanthine / Q000097:blood
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D010936:Plant Extracts; D019820:Xanthine; D019271:Hypoxanthine; D008315:Malondialdehyde; D001205:Ascorbic Acid