title: Coordinated movements of eukaryotic translation initiation factors eIF1, eIF1A, and eIF5 trigger phosphate release from eIF2 in response to start codon recognition by the ribosomal preinitiation complex.
abstract: Accurate recognition of the start codon in an mRNA by the eukaryotic translation preinitiation complex (PIC) is essential for proper gene expression. The process is mediated by eukaryotic translation initiation factors (eIFs) in conjunction with the 40 S ribosomal subunit and (initiator) tRNA(i). Here, we provide evidence that the C-terminal tail (CTT) of eIF1A, which we previously implicated in start codon recognition, moves closer to the N-terminal domain of eIF5 when the PIC encounters an AUG codon. Importantly, this movement is coupled to dissociation of eIF1 from the PIC, a critical event in start codon recognition, and is dependent on the scanning enhancer elements in the eIF1A CTT. The data further indicate that eIF1 dissociation must be accompanied by the movement of the eIF1A CTT toward eIF5 in order to trigger release of phosphate from eIF2, which converts the latter to its GDP-bound state. Our results also suggest that release of eIF1 from the PIC and movement of the CTT of eIF1A are triggered by the same event, most likely accommodation of tRNA(i) in the P site of the 40 S subunit driven by base pairing between the start codon in the mRNA and the anticodon in tRNA(i). Finally, we show that the C-terminal domain of eIF5 is responsible for the factor's activity in antagonizing eIF1 binding to the PIC. Together, our data provide a more complete picture of the chain of molecular events that is triggered when the scanning PIC encounters an AUG start codon in the mRNA.
authors.affiliation: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
authors.forename: Jagpreet S
authors.initials: JS
authors.lastname: Nanda
authors.forename: Adesh K
authors.initials: AK
authors.lastname: Saini
authors.forename: Antonio M
authors.initials: AM
authors.lastname: Muñoz
authors.forename: Alan G
authors.initials: AG
authors.lastname: Hinnebusch
authors.forename: Jon R
authors.initials: JR
authors.lastname: Lorsch
mesh_terms: D001665:Binding Sites; D018387:Codon, Initiator; D003545:Cysteine / Q000235:genetics; D015977:Eukaryotic Initiation Factor-1 / Q000378:metabolism*; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism*; D040061:Eukaryotic Initiation Factor-5 / Q000378:metabolism*; D019793:Fluorescein / Q000494:pharmacology; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D005786:Gene Expression Regulation; D006801:Humans; D007700:Kinetics; D009154:Mutation; D009696:Nucleic Acids / Q000737:chemistry; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D018387:Codon, Initiator; D015977:Eukaryotic Initiation Factor-1; D015852:Eukaryotic Initiation Factor-2; D040061:Eukaryotic Initiation Factor-5; D009696:Nucleic Acids; D011506:Proteins; D012333:RNA, Messenger; C026022:eukaryotic peptide initiation factor-1A; D003545:Cysteine; D019793:Fluorescein