title: [Characterization of human anti-BAFF scFv-Fc that inhibits the activity of BAFF in vivo].
abstract: To investigate the effects of human anti-BAFF scFv-Fc against the hsBAFF, ICR mice were randomly divided into six groups: control, hsBAFF (1 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + Ab (1 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + Ab (2 mg x kg(-1)), hsBAFF (1 mg x kg(-1)) + human IgG (1 mg x kg(-1)) and hsBAFF (1 mg x kg(-1)) + human IgG (2 mg x kg(-1)) groups. The effects of scFv-Fc administration on the proliferation of B lymphocytes were evaluated using an MTT assay. The titres of antibody in the serum and B lymphocytes differentiation were assessed by ELISA and flow cytometry, respectively. The results showed that administration of scFv-Fc to mice injected with hsBAFF significantly prevented human BAFF-induced increases in splenic B cell numbers and serum immunoglobulin levels. Furthermore, this fully human antibody would avoid inducing the human anti-mouse antibody (HAMA) response when used in humans. These findings suggest that the compact antibody may be useful in therapeutic or diagnostic application of the BAFF-associated autoimmune diseases in human.
authors.affiliation: Department of Microbiology and Immunology, Medical School of Southeast University, Nanjing,China. mcao1979@yahoo.com.cn
authors.forename: Meng
authors.initials: M
authors.lastname: Cao
authors.forename: Peng
authors.initials: P
authors.lastname: Cao
authors.forename: Shuang-quan
authors.initials: SQ
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Cheng
authors.forename: Huai-jiang
authors.initials: HJ
authors.lastname: Yan
mesh_terms: D000818:Animals; D053264:B-Cell Activating Factor / Q000276:immunology* / Q000378:metabolism; D001402:B-Lymphocytes / Q000166:cytology; D001835:Body Weight; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000276:immunology* / Q000378:metabolism; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood; D051379:Mice; D008813:Mice, Inbred ICR; D011897:Random Allocation; D011993:Recombinant Fusion Proteins / Q000276:immunology* / Q000378:metabolism; D057127:Single-Chain Antibodies / Q000276:immunology* / Q000378:metabolism; D013154:Spleen / Q000166:cytology
chemical_list: D053264:B-Cell Activating Factor; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011993:Recombinant Fusion Proteins; D057127:Single-Chain Antibodies