title: A shortcut organic dye-based staining method for the detection of DNA both in agarose and polyacrylamide gel electrophoresis.
abstract: In this study, we describe a brief, sensitive and safe organic dye-based staining method for the visualization of DNA both in agarose and polyacrylamide gels by using Victoria Pure Blue BO (VPBBO). Down to 0.8-1.6 ng of λ DNA/HindIII markers in agarose gels and 0.4-0.8 ng of pUC18 DNA/Mspl markers in polyacrylamide gels can be successfully detected within 15 and 10 min by the new developed technique, respectively. Moreover, the mechanism of the VPBBO staining was investigated and further confirmed by electrospray ionization mass spectrometry (ESI-MS) and molecular docking. The results indicated that the interaction between VPBBO and DNA is mainly due to groove binding.
authors.affiliation: Zhejiang Provincial Key Laboratory of Biopharmaceuticals, Wenzhou Medical College, Wenzhou, Zhejiang, China.
authors.forename: Weitao
authors.initials: W
authors.lastname: Cong
authors.forename: Mao
authors.initials: M
authors.lastname: Chen
authors.forename: Zhongxin
authors.initials: Z
authors.lastname: Zhu
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Liu
authors.forename: Jia
authors.initials: J
authors.lastname: Nan
authors.forename: Weijian
authors.initials: W
authors.lastname: Ye
authors.forename: Maowei
authors.initials: M
authors.lastname: Ni
authors.forename: Ting
authors.initials: T
authors.lastname: Zhao
authors.forename: Litai
authors.initials: L
authors.lastname: Jin
mesh_terms: D004396:Coloring Agents / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D004587:Electrophoresis, Agar Gel / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors
chemical_list: D004396:Coloring Agents; D000644:Quaternary Ammonium Compounds; C084160:Victoria blue BO; D004247:DNA