title: The 8D05 parasitism gene of Meloidogyne incognita is required for successful infection of host roots.
abstract: Parasitism genes encode effector proteins that are secreted through the stylet of root-knot nematodes to dramatically modify selected plant cells into giant-cells for feeding. The Mi8D05 parasitism gene previously identified was confirmed to encode a novel protein of 382 amino acids that had only one database homolog identified on contig 2374 within the Meloidogyne hapla genome. Mi8D05 expression peaked in M. incognita parasitic second-stage juveniles within host roots and its encoded protein was limited to the subventral esophageal gland cells that produce proteins secreted from the stylet. Constitutive expression of Mi8D05 in transformed Arabidopsis thaliana plants induced accelerated shoot growth and early flowering but had no visible effects on root growth. Independent lines of transgenic Arabidopsis that expressed a double-stranded RNA complementary to Mi8D05 in host-derived RNA interference (RNAi) tests had up to 90% reduction in infection by M. incognita compared with wild-type control plants, suggesting that Mi8D05 plays a critical role in parasitism by the root-knot nematode. Yeast two-hybrid experiments confirmed the specific interaction of the Mi8D05 protein with plant aquaporin tonoplast intrinsic protein 2 (TIP2) and provided evidence that the Mi8D05 effector may help regulate solute and water transport within giant-cells to promote the parasitic interaction.
authors.affiliation: North Carolina State University, Department of Plant Pathology, Raleigh 27607, USA.
authors.forename: Bingye
authors.initials: B
authors.lastname: Xue
authors.forename: Noureddine
authors.initials: N
authors.lastname: Hamamouch
authors.forename: Chunying
authors.initials: C
authors.lastname: Li
authors.forename: Guozhong
authors.initials: G
authors.lastname: Huang
authors.forename: Richard S
authors.initials: RS
authors.lastname: Hussey
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Baum
authors.forename: Eric L
authors.initials: EL
authors.lastname: Davis
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000235:genetics / Q000469:parasitology* / Q000502:physiology; D001692:Biological Transport; D004198:Disease Susceptibility; D005260:Female; D035264:Flowers / Q000235:genetics / Q000469:parasitology / Q000502:physiology; D015870:Gene Expression; D015726:Giant Cells; D015801:Helminth Proteins / Q000235:genetics* / Q000378:metabolism; D006790:Host-Parasite Interactions; D008018:Life Cycle Stages; D018551:Solanum lycopersicum / Q000235:genetics / Q000469:parasitology* / Q000502:physiology; D008565:Membrane Proteins / Q000378:metabolism*; D010935:Plant Diseases / Q000469:parasitology*; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000469:parasitology / Q000502:physiology; D018520:Plant Shoots / Q000235:genetics / Q000469:parasitology / Q000502:physiology; D030821:Plants, Genetically Modified; D025941:Protein Interaction Mapping; D034622:RNA Interference; D016415:Sequence Alignment; D020798:Two-Hybrid System Techniques; D014431:Tylenchoidea / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D014867:Water / Q000378:metabolism
chemical_list: D015801:Helminth Proteins; D008565:Membrane Proteins; D010940:Plant Proteins; C077491:tonoplast intrinsic protein, plant; D014867:Water