title: [Intensity of cardiac free-radicals processes and expression of glutathione peroxidase and glutathione reductase genes in rats with adrenaline].
abstract: The correlation between changes in activities of glutathione peroxidase and glutathione reductase in heart of rats during development of adrenaline myocarditis and intensity of free radical processes estimated by biochemiluminesce parameters and the content of lipoperoxidation products was demonstrated. The maximal increase of glutathione peroxidase and glutathione reductase activities (in 1.8 and 1.4 times accordingly) was observed t 24 h after the development of the pathological process; this coincided with the maximum intensity of prosesses of free radical oxidation. Using combination of reverse transcriptions with real-time polymerase chain reaction the cardiac mRNA levels of glutathione peroxidase and glutathione reductase genes were determined during the development of adrenaline myocarditis in rats. Analysis of expression of glutathione peroxidase and glutathione reductase genes showed, that the level of this transcripts demonstrated 2,8- and 7,3- increase in rats with adrenaline myocarditis, respectively. Obviously, overexpression of these enzymes can increase the resistance of cardiomyocites to oxidative stress.
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Iskusnykh
authors.forename: T N
authors.initials: TN
authors.lastname: Popova
authors.forename: O S
authors.initials: OS
authors.lastname: Musharova
mesh_terms: D000316:Adrenergic alpha-Agonists / Q000009:adverse effects* / Q000494:pharmacology; D000818:Animals; D004837:Epinephrine / Q000009:adverse effects* / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005979:Glutathione Peroxidase / Q000096:biosynthesis*; D005980:Glutathione Reductase / Q000096:biosynthesis*; D009124:Muscle Proteins / Q000096:biosynthesis*; D009205:Myocarditis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats
chemical_list: D000316:Adrenergic alpha-Agonists; D005609:Free Radicals; D009124:Muscle Proteins; D012333:RNA, Messenger; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D004837:Epinephrine