title: Vector separation of particles and cells using an array of slanted open cavities.
abstract: We present a microfluidic platform for the continuous separation of suspended particles based on their size and settling velocity. The separation method takes advantage of the flow field in the vicinity and inside slanted open cavities. These cavities induce flow along them, which deflects the suspended particles to a different degree depending on the extent to which they penetrate into the cavities. The cumulative deflection in the periodic array ultimately leads to vector chromatography, with the different species in the sample moving in different directions. We demonstrate density and size based separation over a range of flow rates by separating polystyrene and silica particles and show that purities nearing 100% can be achieved for multicomponent mixtures. We also demonstrate the potential of the platform to separate biological cells by fractionating different blood components. We discuss the presence of two regimes, depending on the ratio between the settling velocity and the velocity of the particles across the open cavities. The proposed platform could also integrate additional separative force fields in the direction normal to the plane of the cavities to fractionate specific mixtures based on the distinguishing properties of the component species.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore MD 21218, USA.
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Bernate
authors.forename: Chengxun
authors.initials: C
authors.lastname: Liu
authors.forename: Liesbet
authors.initials: L
authors.lastname: Lagae
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Konstantopoulos
authors.forename: German
authors.initials: G
authors.lastname: Drazer
mesh_terms: D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007962:Leukocytes / Q000166:cytology*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D011137:Polystyrenes; D012822:Silicon Dioxide