title: The potential of endothelial colony-forming cells to improve early graft loss after intraportal islet transplantation.
abstract: Early graft loss in islet transplantation means that a large amount of donor islets is required. Endothelial cells and endothelial colony-forming cells (ECFCs) have been reported to improve instant blood-mediated inflammatory reaction (IBMIR) in vitro. In this study, we examined if ECFC-coated porcine islets would prevent early graft loss in vivo. Human ECFCs were prepared from cord blood and cocultured with islets to make composite grafts. Diabetic nude mice underwent intraportal transplantation. Blood glucose levels were monitored, and morphological examination of the grafts along with analysis of the components of IBMIR and inflammatory reaction were performed with the liver tissues. The ECFC-coated islets significantly decreased blood glucose levels immediately after transplantation compared to the uncoated islets. Composite ECFC islet grafts were observed in the liver sections, associated with a more insulin(+) area compared to that of the uncoated group within 48 h after transplantation. Deposition of CD41a, C5b-9, and CD11b(+) cells was also decreased in the ECFC-coated group. Expression of porcine HMGB1 and mouse TNF-α was increased in the transplantated groups compared to the sham operation group, with a trend of a decreasing trend across the uncoated group, the ECFC-coated group, and the sham group. We demonstrated that the composite ECFC porcine islets transplanted into the portal vein of nude mice improved early graft loss and IBMIR in vivo.
authors.affiliation: Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Hye Seung
authors.initials: HS
authors.lastname: Jung
authors.forename: Min Joo
authors.initials: MJ
authors.lastname: Kim
authors.forename: Shin Hee
authors.initials: SH
authors.lastname: Hong
authors.forename: Ye Jin
authors.initials: YJ
authors.lastname: Lee
authors.forename: Shiane
authors.initials: S
authors.lastname: Kang
authors.forename: Hakmo
authors.initials: H
authors.lastname: Lee
authors.forename: Sung Soo
authors.initials: SS
authors.lastname: Chung
authors.forename: Joong Shin
authors.initials: JS
authors.lastname: Park
authors.forename: Kyong Soo
authors.initials: KS
authors.lastname: Park
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D042783:Endothelial Cells / Q000166:cytology* / Q000276:immunology; D006084:Graft Rejection / Q000276:immunology / Q000517:prevention & control*; D024243:HMGB1 Protein / Q000032:analysis; D006801:Humans; D007249:Inflammation / Q000276:immunology; D007515:Islets of Langerhans / Q000166:cytology* / Q000276:immunology; D016381:Islets of Langerhans Transplantation / Q000276:immunology / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D013234:Stem Cells / Q000166:cytology* / Q000276:immunology; D013552:Swine; D014183:Transplantation, Heterologous; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D001786:Blood Glucose; D024243:HMGB1 Protein; D014409:Tumor Necrosis Factor-alpha