title: Effects of lercanidipine on bone density and bone repair in spontaneously hypertensive rats.
abstract: OBJECTIVE
  To evaluate the effects of the lercanidipine on bone healing (BH) and bone density (BD) in the tibiae of spontaneously hypertensive rats (SHR), using histometric and tartrate-resistant acid phosphatase (TRAP) expression analyses.
  
  
  METHODS
  Wistar and SHR were assigned to one of the following groups: normotensive rats (NTR) (n = 15), untreated SHR (n = 15), and lercanidipine-treated SHR (n = 15). The latter group was treated daily with lercanidipine for 6 weeks. Two weeks after the beginning of drug administration, a critical-sized surgical defect was created in the right tibia of all groups, whereas the contralateral tibia remained without defect. The animals were killed 30 days after the creation of the bone defect.
  
  
  RESULTS
  There were no significant differences among the groups for BH, trabecular BD, and the number of TRAP+ cells in the newly formed cortical bone (P > 0.05). SHR presented significantly lower cortical BD and increased cortical levels of TRAP+ cells, when compared with NTR and lercanidipine-treated SHR (P < 0.05).
  
  
  CONCLUSIONS
  SHR presented a lower cortical BD and increased levels of TRAP+ cells. In addition, the treatment of SHR with lercanidipine during 6 weeks was able to revert the deleterious effects of hypertension on cortical BD and on the number of TRAP+ cells in the tibia of SHR.
authors.affiliation: Department of Periodontics, Dental Research Division, Guarulhos University, Guarulhos, São Paulo, Brazil. mfbastos@prof.ung.br
authors.forename: Marta Ferreira
authors.initials: MF
authors.lastname: Bastos
authors.forename: Isaac
authors.initials: I
authors.lastname: Vasconcelos de Araújo
authors.forename: Felipe
authors.initials: F
authors.lastname: Vilhena Brilhante
authors.forename: Amanda Gonçalves
authors.initials: AG
authors.lastname: Pires
authors.forename: Tiago Eduardo
authors.initials: TE
authors.lastname: Dias Gonçalves
authors.forename: Marcelo Henrique
authors.initials: MH
authors.lastname: Napimoga
authors.forename: Marcelo Rocha
authors.initials: MR
authors.lastname: Marques
authors.forename: Poliana Mendes
authors.initials: PM
authors.lastname: Duarte
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D000959:Antihypertensive Agents / Q000627:therapeutic use*; D015415:Biomarkers / Q000032:analysis; D015519:Bone Density / Q000187:drug effects*; D001847:Bone Diseases / Q000473:pathology / Q000503:physiopathology; D001861:Bone Regeneration / Q000187:drug effects*; D002121:Calcium Channel Blockers / Q000627:therapeutic use*; D004095:Dihydropyridines / Q000627:therapeutic use*; D006973:Hypertension / Q000188:drug therapy / Q000503:physiopathology; D007091:Image Processing, Computer-Assisted; D007527:Isoenzymes / Q000032:analysis; D008297:Male; D051381:Rats; D011918:Rats, Inbred SHR; D017208:Rats, Wistar; D000071681:Tartrate-Resistant Acid Phosphatase; D013977:Tibia / Q000187:drug effects* / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000959:Antihypertensive Agents; D015415:Biomarkers; D002121:Calcium Channel Blockers; D004095:Dihydropyridines; D007527:Isoenzymes; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; C060343:lercanidipine