title: Expression of antimicrobial peptides by bovine endothelial cells.
abstract: In this work, we explore if Gram-positive bacteria as Staphylococcus aureus or Gram-negative bacteria components as LPS, can induce the expression of seven antimicrobial peptides (AP) in an immortalized bovine umbilical vein endothelial cell line (BUVEC). By qPCR we determined the constitutive expression of all the AP evaluated. The stimulation with S. aureus or LPS induced the expression of lingual antimicrobial peptide (LAP), bovine β-defensin 1 (DEFB1) and bovine neutrophil β-defensin 4 (BNBD4). This expression was regulated by the autocrine production of tumor necrosis factor-α (TNF-α), indicating that bovine endothelial cells (EC) can play a more active role during infection.
authors.affiliation: Centro Multidisciplinario de Estudios en Biotecnología-Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolás de Hidalgo, La Palma, Tarímbaro, Michoacán, Mexico.
authors.forename: Nayeli
authors.initials: N
authors.lastname: Alva-Murillo
authors.forename: Ana D
authors.initials: AD
authors.lastname: Téllez-Pérez
authors.forename: Eduardo
authors.initials: E
authors.lastname: Sagrero-Cisneros
authors.forename: Joel E
authors.initials: JE
authors.lastname: López-Meza
authors.forename: Alejandra
authors.initials: A
authors.lastname: Ochoa-Zarzosa
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000096:biosynthesis / Q000235:genetics; D019898:Autocrine Communication; D002417:Cattle; D002461:Cell Line, Transformed / Q000187:drug effects / Q000378:metabolism; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D008070:Lipopolysaccharides / Q000494:pharmacology; D008414:Mastitis, Bovine / Q000382:microbiology; D013211:Staphylococcus aureus / Q000276:immunology / Q000302:isolation & purification; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics; D014471:Umbilical Veins; D023083:beta-Defensins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D023181:Antimicrobial Cationic Peptides; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D023083:beta-Defensins; C092480:lingual antimicrobial peptide; C440499:lipopolysaccharide, Escherichia coli O111 B4; C068629:tracheal antimicrobial peptide, bovine