title: Temperature dependence of the production of staphylococcal enterotoxin A by Staphylococcus aureus.
abstract: We incubated 11 strains of Staphylococcus aureus in a brain heart infusion broth at 10-37 °C with two inoculum sizes and examined their enterotoxin A (SEA) production by a Western blot analysis to clarify the effect of incubation temperature on SEA production. Although SEA was detected in the exponential phase at 15-37 °C, it was also detected in the stationary or death phase at 10 °C. The maximal SEA concentrations of most strains increased as the temperature was increased, although some strains produced as much at 15 °C and 20 °C as they did at 37 °C. The maximal SEA concentration was definitely lowest at 10 °C, and as the temperature was increased, the production rate increased. However, a relationship between the production rates at the two different temperatures was not apparent. Some strains produced more SEA at 10-20 °C with a smaller inoculum size than with a larger one. SEA production therefore did not necessarily depend on the incubation temperature, and it would be difficult to predict at 10 °C and 15 °C from the production at 37 °C.
authors.affiliation: Department of Nutrition and Food Science, Ochanomizu University, Tokyo, Japan.
authors.forename: Satomi
authors.initials: S
authors.lastname: Tsutsuura
authors.forename: Yuko
authors.initials: Y
authors.lastname: Shimamura
authors.forename: Masatsune
authors.initials: M
authors.lastname: Murata
mesh_terms: D015153:Blotting, Western; D003470:Culture Media / Q000737:chemistry; D004768:Enterotoxins / Q000096:biosynthesis*; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000254:growth & development / Q000378:metabolism*; D013696:Temperature
chemical_list: D003470:Culture Media; D004768:Enterotoxins; C026066:enterotoxin A, Staphylococcal