title: [Susceptibility to fluconazole of clinical interest yeasts: new breakpoints].
abstract: BACKGROUND
  Recently, Pfaller et al (Drug Resist Update 2010; 13:180-95), have proposed new breakpoints for determining the in vitro susceptibility to fluconazole of Candida albicans, C. parapsilosis and C. tropicalis. The aim of this study was to establish the variations in sensitivity of these species applying these breakpoints, in relation to those of the Clinical and Laboratory Standards Institute (CLSI).
  
  
  METHODS
  We analyzed 112 strains of Candida: 49 C. albicans, 40 C. parapsilosis and 23 C. tropicalis. Susceptibility to fluconazole was performed by the method Sensititre YeastOne. The breakpoints used to determine the minimum inhibitory concentration (MIC) were identified by CLSI and the ones proposed by Pfaller et al.
  
  
  RESULTS
  According to the CLSI criteria, all isolates were susceptible to fluconazole. MIC50 and MIC90 were 0.5 mg/L and 2 mg/L for C. albicans and C. parapsilosis, 0.5 mg/L and 1 mg/L for C. tropicalis. With the new criteria, 109 (97%) strains were susceptible. Variations were seen in C. albicans, with 3 strains (6%) susceptible dose-dependent.
  
  
  CONCLUSIONS
  When applying the breakpoints recommended by Pfaller et al, and EUCAST, the number of fluconazole-susceptible strains decreased according to the CLSI criteria, especially C. albicans.
authors.affiliation: Servicio de Microbiología y Parasitología, Hospital Universitario Puerta del Mar, Spain.
authors.forename: Lidia
authors.initials: L
authors.lastname: García-Agudo
authors.forename: Pedro
authors.initials: P
authors.lastname: García-Martos
authors.forename: Pilar
authors.initials: P
authors.lastname: Marín-Casanova
authors.forename: Manuel
authors.initials: M
authors.lastname: Rodríguez-Iglesias
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects; D041022:Candida tropicalis / Q000187:drug effects; D025141:Drug Resistance, Fungal; D015725:Fluconazole / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000592:standards*; D013045:Species Specificity
chemical_list: D000935:Antifungal Agents; D015725:Fluconazole