title: Study on noninvasive laboratory tests for fibrosis in chronic HBV infection and their evaluation.
abstract: BACKGROUND
  Noninvasive laboratory tests have been widely used in the diagnosis of liver fibrosis. This study was designed to evaluate the diagnostic value of the four serum markers detected on a JETLIA-962 chemiluminescence analyzer and the FibroTest index for chronic hepatitis B (CHB) related liver fibrosis.
  
  
  METHODS
  A JETLIA-962 chemiluminescence analyzer was used to measure the hyaluronic acid (HA), laminin (LN), procollagen III N-terminal peptide (PIIINP), and type IV collagen (CIV) contents in the sera of CHB patients and controls.
  
  
  RESULTS
  In our research, it was found that serum HA, LN, PIIINP, and CIV concentrations and the FibroTest index were correlated with fibrosis stage. The FibroTest index had the largest area under the receiver operating characteristic (ROC) curve for fibrosis and cirrhosis (0.80 and 0.776, respectively). The five indices together were able to exclude fibrosis with a negative likelihood ratio <0.1. The logistic regression equations for diagnosing liver fibrosis and early cirrhosis were separately established.
  
  
  CONCLUSIONS
  We found that the JETLIA-962 chemiluminescence analyzer was of great value in diagnosis of liver fibrosis. Combining five indices improved the diagnostic efficiency and reduced the incidences of unnecessary liver biopsies. Two logistic regression equations established may be helpful in diagnosing liver fibrosis and early cirrhosis, which need to be further evaluated.
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of Fujian Medical University, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Can
authors.initials: C
authors.lastname: Liu
authors.forename: Huijuan
authors.initials: H
authors.lastname: Chen
authors.forename: Qicai
authors.initials: Q
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.forename: Qishui
authors.initials: Q
authors.lastname: Ou
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D024141:Collagen Type IV / Q000097:blood; D005260:Female; D019694:Hepatitis B, Chronic / Q000097:blood* / Q000473:pathology*; D006801:Humans; D006820:Hyaluronic Acid / Q000097:blood; D007797:Laminin / Q000097:blood; D008103:Liver Cirrhosis / Q000097:blood* / Q000473:pathology / Q000821:virology*; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000097:blood; D011347:Procollagen / Q000097:blood; D012372:ROC Curve
chemical_list: D015415:Biomarkers; D024141:Collagen Type IV; D007797:Laminin; D010446:Peptide Fragments; D011347:Procollagen; C041876:procollagen Type III-N-terminal peptide; D006820:Hyaluronic Acid