title: A CpG-containing oligodeoxynucleotide adjuvant for acellular pertussis vaccine improves the protective response against Bordetella pertussis.
abstract: We investigated the adjuvant effect of CpG ODN alone or in combination with aluminum hydroxide on the immune response to the three main antigens presented in current acellular pertussis vaccines: pertussis toxoid, filamentous haemagglutinin and pertactin. The development of protection in mice was investigated for the intra-peritoneal and intra-nasal immunisation routes. The results showed that CpG ODN alone, or in combination with aluminum hydroxide, gave enhancement in anti-pertussis toxin, anti- filamentous haemagglutinin and especially anti-pertactin titers after mucosal immunisation. Higher macrophage NO levels indicating activation were found when the antigens were co-formulated with CpG ODN. Vaccines containing CpG ODN gave enhanced humoral and CMI responses with a shift toward Th-1 and increased protection against challenge infection with B.pertussis in mice.
authors.affiliation: National Institute for Biological Standards and Control (NIBSC); South Mimms, Potters Bar, UK.
authors.forename: Catpagavalli
authors.initials: C
authors.lastname: Asokanathan
authors.forename: Michael
authors.initials: M
authors.lastname: Corbel
authors.forename: Dorothy
authors.initials: D
authors.lastname: Xing
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology; D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000281:Administration, Intranasal; D000536:Aluminum Hydroxide / Q000008:administration & dosage; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001886:Bordetella pertussis / Q000276:immunology*; D005260:Female; D007274:Injections, Intraperitoneal; D008264:Macrophages / Q000276:immunology; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage*; D037342:Pertussis Toxin / Q000276:immunology; D010567:Pertussis Vaccine / Q000008:administration & dosage* / Q000276:immunology*; D022282:Vaccines, Acellular / Q000008:administration & dosage / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000276:immunology
chemical_list: D018829:Adhesins, Bacterial; D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; C408982:CPG-oligonucleotide; D009838:Oligodeoxyribonucleotides; D010567:Pertussis Vaccine; D022282:Vaccines, Acellular; D010566:Virulence Factors, Bordetella; C065273:filamentous hemagglutinin adhesin, Bordetella pertussis; D009569:Nitric Oxide; D000536:Aluminum Hydroxide; C068929:pertactin; D037342:Pertussis Toxin
keywords: Bordetella pertussis; CpG ODN; acellular pertussis vaccine; adjuvant