title: Simultaneous determination of a novel anxiolytic agent buagafuran and one metabolite in human plasma by ultra-performance liquid chromatography-tandem mass spectrometry.
abstract: A robust and validated ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method has been developed for the simultaneous determination of buagafuran and one metabolite (M1) in human plasma. The two analytes were extracted from plasma samples using tert-butyl methyl ether after addition of the internal standard and chromatographed on an Acquity UPLC BEH C18 column (2.1mm×50mm, 1.7μm) thermostatted at 35°C with methanol-water (75:25, v/v) as the mobile phase at an isocratic flow rate of 0.4mL/min. The detection was performed on an API 5500 mass spectrometer coupled with electrospray ionization (ESI) source in positive mode. The multiple reactions monitoring (MRM) transitions of m/z 245.2→109.1 and m/z 279.1→243.1 were used to quantify buagafuran and M1, respectively. The assay was validated over the concentration range of 0.5-200ng/mL for the two analytes. Precision and accuracy are in accordance with the generally accepted criteria for bioanalytical methods. The extraction recovery and the matrix effect were investigated. This method was successfully applied to support a clinical study where multiple oral doses were administrated to healthy Chinese volunteers to investigate the pharmacokinetics, safety and tolerability of buagafuran.
authors.affiliation: Clinical Pharmacology Research Center, Peking Union Medical College Hospital and Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Yang
authors.forename: Hongyun
authors.initials: H
authors.lastname: Wang
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhao
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
authors.forename: Ji
authors.initials: J
authors.lastname: Jiang
authors.forename: Pei
authors.initials: P
authors.lastname: Hu
mesh_terms: D000284:Administration, Oral; D014151:Anti-Anxiety Agents / Q000008:administration & dosage / Q000009:adverse effects / Q000493:pharmacokinetics*; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004311:Double-Blind Method; D006801:Humans; D015203:Reproducibility of Results; D012717:Sesquiterpenes / Q000008:administration & dosage / Q000009:adverse effects / Q000493:pharmacokinetics*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D014151:Anti-Anxiety Agents; D012717:Sesquiterpenes; C530322:buagafuran