title: An endothelial cell-specific requirement for the UL133-UL138 locus of human cytomegalovirus for efficient virus maturation.
abstract: Human cytomegalovirus (HCMV) infects a variety of cell types in humans, resulting in a varied pathogenesis in the immunocompromised host. Endothelial cells (ECs) are considered an important target of HCMV infection that may contribute to viral pathogenesis. Although the viral determinants important for entry into ECs are well defined, the molecular determinants regulating postentry tropism in ECs are not known. We previously identified the UL133-UL138 locus encoded within the clinical strain-specific ULb' region of the HCMV genome as important for the latent infection in CD34(+) hematopoietic progenitor cells (HPCs). Interestingly, this locus, while dispensable for replication in fibroblasts, was required for efficient replication in ECs infected with the TB40E or fusion-inducing factor X (FIX) HCMV strains. ECs infected with a virus lacking the entire locus (UL133-UL138(NULL) virus) complete the immediate-early and early phases of infection but are defective for infectious progeny virus production. ECs infected with UL133-UL138(NULL) virus exhibited striking differences in the organization of intracellular membranes and in the assembly of mature virions relative to ECs infected with wild-type (WT) virus. In UL133-UL138(NULL) virus-infected ECs, Golgi stacks were disrupted, and the viral assembly compartment characteristic of HCMV infection failed to form. Further, progeny virions in UL133-UL138(NULL) virus-infected ECs inefficiently acquired the virion tegument and secondary envelope. These defects were specific to infection in ECs and not observed in fibroblasts infected with UL133-UL138(NULL) virus, suggesting an EC-specific requirement for the UL133-UL138 locus for late stages of replication. To our knowledge, the UL133-UL138 locus represents the first cell-type-dependent, postentry tropism determinant required for viral maturation.
authors.affiliation: The University of Arizona, Tucson, AZ, USA.
authors.forename: Farah
authors.initials: F
authors.lastname: Bughio
authors.forename: David A
authors.initials: DA
authors.lastname: Elliott
authors.forename: Felicia
authors.initials: F
authors.lastname: Goodrum
mesh_terms: D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics / Q000502:physiology*; D042783:Endothelial Cells / Q000821:virology*; D005347:Fibroblasts / Q000821:virology; D055786:Gene Knockout Techniques; D054884:Host-Pathogen Interactions; D006801:Humans; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D019065:Virus Assembly; D014779:Virus Replication
chemical_list: D014764:Viral Proteins