title: Med19 promotes bone metastasis and invasiveness of bladder urothelial carcinoma via bone morphogenetic protein 2.
abstract: Bladder cancer (BCa) remained a major health problem. Med19 was related to tumor growth of BCa. Bone morphogenetic proteins (BMPs) were reported to be critical in bone metastasis of cancer. We therefore investigated the relations between Med19 and BMPs in BCa and their effect on bone metastasis of BCa. Bladder cancer cell lines were cultured and interfered with Med19 shRNA and control. Expressions of BMP-1, BMP-2, BMP-4, BMP-5, BMP-6, BMP-7, BMP-9, and BMP-15 were studied between 2 groups. Fifty-two BCa samples were included for immunohistochemical staining of Med19 and BMP-2. Expressions were scored and studied statistically. Invasiveness was studied with Transwell assay. Silencing or Med19 in BCa cells induced altered expressions of BMPs. Increased expressions of BMP-1, BMP-4, BMP-6, BMP-7, and BMP-15 and decreased expressions of BMP-2, BMP-5, and BMP-9 were noticed, but only BMP-2 reached statistical significance. Expressions of Med19 and BMP-2 were significantly higher in cases with bone metastasis and were positively correlated in cases with bone metastasis and muscle invasion. Med19 is a critical factor involved in the invasiveness and promotion of bone metastasis of BCa, possibly via BMP-2.
authors.affiliation: Department of Urology, Huashan Hospital, Fudan University, Shanghai 200040, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wen
authors.forename: Chen-chen
authors.initials: CC
authors.lastname: Feng
authors.forename: Guan-xiong
authors.initials: GX
authors.lastname: Ding
authors.forename: Dong-liang
authors.initials: DL
authors.lastname: Meng
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ding
authors.forename: Zu-jun
authors.initials: ZJ
authors.lastname: Fang
authors.forename: Guo-wei
authors.initials: GW
authors.lastname: Xia
authors.forename: Gang
authors.initials: G
authors.lastname: Xu
authors.forename: Hao-wen
authors.initials: HW
authors.lastname: Jiang
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism / Q000556:secondary*; D002295:Carcinoma, Transitional Cell / Q000235:genetics / Q000378:metabolism / Q000556:secondary*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008297:Male; D056892:Mediator Complex / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D019459:Urothelium / Q000378:metabolism / Q000473:pathology
chemical_list: C525718:BMP2 protein, human; D014408:Biomarkers, Tumor; D055396:Bone Morphogenetic Protein 2; C558319:MED19 protein, human; D056892:Mediator Complex; D034741:RNA, Small Interfering