title: Demonstration of specific binding of heparin to Plasmodium falciparum-infected vs. non-infected red blood cells by single-molecule force spectroscopy.
abstract: Glycosaminoglycans (GAGs) play an important role in the sequestration of Plasmodium falciparum-infected red blood cells (pRBCs) in the microvascular endothelium of different tissues, as well as in the formation of small clusters (rosettes) between infected and non-infected red blood cells (RBCs). Both sequestration and rosetting have been recognized as characteristic events in severe malaria. Here we have used heparin and pRBCs infected by the 3D7 strain of P. falciparum as a model to study GAG-pRBC interactions. Fluorescence microscopy and fluorescence-assisted cell sorting assays have shown that exogenously added heparin has binding specificity for pRBCs (preferentially for those infected with late forms of the parasite) vs. RBCs. Heparin-pRBC adhesion has been probed by single-molecule force spectroscopy, obtaining an average binding force ranging between 28 and 46 pN depending on the loading rate. No significant binding of heparin to non-infected RBCs has been observed in control experiments. This work represents the first approach to quantitatively evaluate GAG-pRBC molecular interactions at the individual molecule level.
authors.affiliation: Nanobioengineering Group, Institute for Bioengineering of Catalonia (IBEC), Baldiri Reixac 10-12, Barcelona E08028, Spain.
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Valle-Delgado
authors.forename: Patricia
authors.initials: P
authors.lastname: Urbán
authors.forename: Xavier
authors.initials: X
authors.lastname: Fernàndez-Busquets
mesh_terms: D002448:Cell Adhesion; D002460:Cell Line; D004912:Erythrocytes / Q000378:metabolism; D006025:Glycosaminoglycans / Q000737:chemistry / Q000378:metabolism; D006493:Heparin / Q000378:metabolism*; D006801:Humans; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D010963:Plasmodium falciparum / Q000378:metabolism*; D011107:Polylysine / Q000737:chemistry
chemical_list: D006025:Glycosaminoglycans; D011107:Polylysine; D006493:Heparin