title: The role of aldehyde/alcohol dehydrogenase (AdhE) in ethanol production from glycerol by Klebsiella pneumoniae.
abstract: Transcriptome analysis of a K. pneumoniae GEM167 mutant strain derived by irradiation with gamma rays, which exhibited high-level production of ethanol from glycerol, showed that the mutant expressed AdhE at a high level. Ethanol production decreased significantly, from 8.8 to 0.5 g l(-1), when an adhE-deficient derivative of that strain was grown on glycerol. Bacterial growth was also reduced under such conditions, showing that AdhE plays a critical role in maintenance of redox balance by catalyzing ethanol production. Overexpression of AdhE enhanced ethanol production, from pure or crude glycerol, to a maximal level of 31.9 g l(-1) under fed-batch fermentation conditions; this is the highest level of ethanol production from glycerol reported to date.
authors.affiliation: Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, Jeonbuk 580-185, Korea.
authors.forename: Baek-Rock
authors.initials: BR
authors.lastname: Oh
authors.forename: Won-Kyung
authors.initials: WK
authors.lastname: Hong
authors.forename: Sun-Yeon
authors.initials: SY
authors.lastname: Heo
authors.forename: Min-ho
authors.initials: MH
authors.lastname: Joe
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Seo
authors.forename: Chul Ho
authors.initials: CH
authors.lastname: Kim
mesh_terms: D000426:Alcohol Dehydrogenase / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D000444:Aldehyde Dehydrogenase / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005816:Genetic Complementation Test; D005990:Glycerol / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D017384:Sequence Deletion / Q000235:genetics
chemical_list: D000431:Ethanol; D000426:Alcohol Dehydrogenase; D000444:Aldehyde Dehydrogenase; D005990:Glycerol