title: [Improvement of the method of isolation of hydrogen-forming bacteria of Clostridium genus].
abstract: The method of isolation and quantitative account of pure cultures of obligate anaerobic hydrogen-forming clostridia is improved. A strain of hydrogen-forming bacteria Clostridium sp. BY-11 has been isolated from the association of sporulating bacteria. Quantitative indices of hydrogen synthesis and starch fermentation have been determined when growing the strain in the liquid medium. Concentration of H2 in the gas phase was 49%, microorganisms synthesized 128 1 of H2 from 1 kg of starch, the mass of starch decreased 7 times for 6 days. The mentioned indices for hydrogen synthesis and starch fermentation and for other organic model substrates in the future are the basis for creating the industrial biotechnology for production of hydrogen as the energy carrier under disposal of ecologically dangerous solid food waste.
authors.forename: I R
authors.initials: IR
authors.lastname: Pritula
authors.forename: A B
authors.initials: AB
authors.lastname: Tashirev
mesh_terms: D000693:Anaerobiosis; D056804:Biofuels; D001709:Biotechnology / Q000379:methods*; D003013:Clostridium / Q000302:isolation & purification* / Q000502:physiology; D003470:Culture Media; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D013213:Starch / Q000378:metabolism*
chemical_list: D056804:Biofuels; D003470:Culture Media; D006859:Hydrogen; D013213:Starch