title: IL-6 regulated stress-induced Rex-1 expression in stem cells from human exfoliated deciduous teeth.
abstract: OBJECTIVE
  To investigate the effect of mechanical stress on stem cells from human exfoliated deciduous teeth (SHED).
  
  
  METHODS
  Cells were stimulated with mechanical stress ranging from 0 to 2.5 g cm⁻² for 2 h in serum-free condition. The expression of stem cell markers was examined by polymerase chain reaction and immunocytochemistry. The molecular mechanisms of these effects were investigated by means of inhibitors and siRNA.
  
  
  RESULTS
  Mechanical stress induced Rex-1 expression in a force-dependent manner. The upregulation of interleukin-6 (IL-6) was also observed. Application of neutralizing antibody against IL-6 and IL-6 siRNA could attenuate the mechanical stress-induced Rex-1 expression. In addition, the increase in mRNA levels of Rex-1 was observed after treating SHED with an exogenous IL-6. This inductive effect was attenuated by JAK inhibitor, suggesting the involvement of JAK signaling pathway.
  
  
  CONCLUSIONS
  These results suggest the role of mechanical stress in the regulation of stemness via IL-6-Rex-1 interaction.
authors.affiliation: Research Unit of Mineralized Tissue, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
authors.forename: N
authors.initials: N
authors.lastname: Govitvattana
authors.forename: T
authors.initials: T
authors.lastname: Osathanon
authors.forename: S
authors.initials: S
authors.lastname: Taebunpakul
authors.forename: P
authors.initials: P
authors.lastname: Pavasant
mesh_terms: D050156:Adipogenesis / Q000502:physiology; D001696:Biomechanical Phenomena; D002113:Calcification, Physiologic / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D020868:Gene Silencing; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics / Q000502:physiology*; D053612:Janus Kinases / Q000502:physiology; D051741:Kruppel-Like Transcription Factors / Q000502:physiology*; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D055495:Neurogenesis / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology; D034741:RNA, Small Interfering / Q000235:genetics; D013314:Stress, Mechanical; D014094:Tooth, Deciduous / Q000166:cytology*; D015854:Up-Regulation; D016335:Zinc Fingers / Q000502:physiology*
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6; D051741:Kruppel-Like Transcription Factors; D034741:RNA, Small Interfering; C507976:ZFP42 protein, human; D053612:Janus Kinases
keywords: IL-6; Rex-1; mechanical stress; stem cells