title: Enhanced ethyl butyrate production using immobilized lipase.
abstract: In this study, the production of ethyl butyrate was investigated by using immobilized lipase enzyme in shake flasks. In order to determine optimum conditions for the production, response surface methodology was used. The model indicated the optimum conditions for maximum conversion (9.1%) at the 0.31 M substrate concentration, acid- alcohol molar ratio of 0.49, immobilized enzyme 25% (w/v) at 35°C, for 3 hours which were in good agreement with the experimental value. At the end of the 55 hours conversion was obtained as 61.3%. When Na2HPO4 was used in reaction medium conversion increased to 90.3% for 55 hours.
authors.affiliation: Gazi University, Faculty of Science, Chemistry Department , Ankara , Turkey.
authors.forename: Selma
authors.initials: S
authors.lastname: Ateş
authors.forename: Burcu
authors.initials: B
authors.lastname: Türk
authors.forename: Emine
authors.initials: E
authors.lastname: Bayraktar
authors.forename: Afife
authors.initials: A
authors.lastname: Güvenç
mesh_terms: D000704:Analysis of Variance; D002087:Butyrates / Q000138:chemical synthesis*; D020148:Butyric Acid / Q000138:chemical synthesis*; D004800:Enzymes, Immobilized / Q000378:metabolism; D000431:Ethanol / Q000138:chemical synthesis*; D006801:Humans; D008049:Lipase / Q000378:metabolism; D015233:Models, Statistical
chemical_list: D002087:Butyrates; D004800:Enzymes, Immobilized; D020148:Butyric Acid; D000431:Ethanol; D008049:Lipase; C045572:ethyl butyrate