title: EGF signalling and rapamycin-mediated mTOR inhibition in glioblastoma multiforme evaluated by phospho-specific flow cytometry.
abstract: Development of novel patient stratification tools for cancer is a challenge that require advanced molecular screening and a detailed understanding of tumour signalling networks. Here, we apply phospho-specific flow cytometry for signal profiling of primary glioblastoma tumours after preservation of single-cell phosphorylation status as a strategy for evaluation of tumour signalling potential and assessment of rapamycin-mediated mTOR inhibition. The method has already enhanced insight into cancers and disorders of the immune system, and our study demonstrate a great potential to improve the understanding of aberrant signalling in glioblastoma and other solid tumours.
authors.affiliation: The Biotechnology Centre of Oslo and Centre for Molecular Medicine Norway, Nordic EMBL Partnership, University of Oslo, Gaustadalléen 21, 0349 Oslo, Norway.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Cornez
authors.forename: Mrinal
authors.initials: M
authors.lastname: Joel
authors.forename: Kjetil
authors.initials: K
authors.lastname: Taskén
authors.forename: Iver A
authors.initials: IA
authors.lastname: Langmoen
authors.forename: Joel C
authors.initials: JC
authors.lastname: Glover
authors.forename: Torunn
authors.initials: T
authors.lastname: Berge
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001483:Base Sequence; D001932:Brain Neoplasms / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000378:metabolism*; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000235:genetics; D015723:Gene Library; D005909:Glioblastoma / Q000473:pathology*; D006801:Humans; D010766:Phosphorylation / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects*; D020123:Sirolimus / Q000494:pharmacology*; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D000903:Antibiotics, Antineoplastic; D004815:Epidermal Growth Factor; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases; D020123:Sirolimus