title: Quantitative and qualitative preparations of bacterial outer membrane vesicles.
abstract: Gram-negative bacterial outer membrane vesicle production and function have been studied using a variety of quantitative and qualitative methods. These types of analyses can be hampered by the use of impure vesicle preparations. Here we describe a set of techniques that are useful for the quantitative analysis of vesicle production and for preparative yields of highly purified vesicles for studies of vesicle function or composition. Procedures and advice are also included for the purification of vesicles from encapsulated and low-yield strains.
authors.affiliation: Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC, USA.
authors.forename: Halima
authors.initials: H
authors.lastname: Chutkan
authors.forename: Ian
authors.initials: I
authors.lastname: Macdonald
authors.forename: Andrew
authors.initials: A
authors.lastname: Manning
authors.forename: Meta J
authors.initials: MJ
authors.lastname: Kuehn
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006090:Gram-Negative Bacteria / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron
chemical_list: D001425:Bacterial Outer Membrane Proteins