title: Molecular recognition force spectroscopy study of the dynamic interaction between aptamer GBI-10 and extracellular matrix protein tenascin-C on human glioblastoma cell.
abstract: Molecular recognition force spectroscopy (MR-FS) was applied to investigate the dynamic interaction between aptamer GBI-10 and tenascin-C (TN-C) on human glioblastoma cell surface at single-molecule level. The unbinding force between aptamer GBI-10 and TN-C was 39 pN at the loading rate of 0.3 nN sec⁻¹. A series of kinetic parameters concerning interaction process such as the unbinding force f(u) , the association rate constant k(on) , dissociation rate constant at zero force k(off) , and dissociation constant K(D) for aptamer GBI-10/TN-C complexes were acquired. In addition, the interaction of aptamer GBI-10 with TN-C depended on the presence of Mg²⁺. This work demonstrates that MR-FS can be used as an attractive tool for exploring the interaction forces and dynamic process of aptamer and ligand at the single-molecule level. As a future perspective, MR-FS may be used as a potential diagnostic and therapeutic tool by combining with other techniques.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Li
authors.forename: Haiyan
authors.initials: H
authors.lastname: Qiao
authors.forename: Wei
authors.initials: W
authors.lastname: Yan
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Chunyan
authors.initials: C
authors.lastname: Xing
authors.forename: Hongda
authors.initials: H
authors.lastname: Wang
authors.forename: Bailin
authors.initials: B
authors.lastname: Zhang
authors.forename: Jilin
authors.initials: J
authors.lastname: Tang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D045744:Cell Line, Tumor; D016326:Extracellular Matrix Proteins / Q000737:chemistry* / Q000378:metabolism; D005909:Glioblastoma / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008024:Ligands; D008274:Magnesium / Q000737:chemistry; D018625:Microscopy, Atomic Force / Q000379:methods*; D056004:Molecular Dynamics Simulation; D011485:Protein Binding; D013057:Spectrum Analysis / Q000379:methods*; D019063:Tenascin / Q000737:chemistry* / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; D016326:Extracellular Matrix Proteins; D008024:Ligands; D019063:Tenascin; D008274:Magnesium