title: Isolation and identification of mannose-binding proteins and estimation of their abundance in sera from hepatocellular carcinoma patients.
abstract: The interaction of glycan-binding proteins (GBPs) and glycans plays a significant biological role that ranges from cell-cell recognition to cell trafficking, and glycoprotein targeting. The anomalies of GBPs related to the types and/or quantities were not clearly known in cancer incidence. It is imperative to identify and annotate the GBPs related with the canceration. Here the mannose-binding proteins (MBPs) from the clinical sera were isolated and identified by the mannose-magnetic particle conjugates and the high-accuracy MS analysis. Seventy-five MBPs from normal donors' sera and 79 MBPs from hepatocellular carcinoma patients' sera were identified and annotated. By using the stringent criteria of exponentially modified protein abundance index (emPAI) quantification, 12 MBPs were estimated to be significantly upregulated (emPAI ratio > 4) and nine MBPs were estimated to be significantly downregulated (emPAI ratio < 0.25) in the hepatocellular carcinoma sera. Real-time quantitative PCR, Western blotting, and protein microarrays were also used to confirm the altered MBPs expression level and the specific binding between the isolated MBPs and mannose. The sequence recognition motifs and structure preference of the isolated MBPs were characterized. The functional enrichment analysis revealed that over 57% of the isolated MBPs were binding protein and the upregulated MBPs were involved in cell death, tumor progression, and macromolecular complex remodeling.
authors.affiliation: Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi'an, China.
authors.forename: Ganglong
authors.initials: G
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Chu
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Xiuxuan
authors.initials: X
authors.lastname: Sun
authors.forename: Tanxi
authors.initials: T
authors.lastname: Cai
authors.forename: Liuyi
authors.initials: L
authors.lastname: Dang
authors.forename: Qinzhe
authors.initials: Q
authors.lastname: Wang
authors.forename: Hanjie
authors.initials: H
authors.lastname: Yu
authors.forename: Yaogang
authors.initials: Y
authors.lastname: Zhong
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Chen
authors.forename: Fuquan
authors.initials: F
authors.lastname: Yang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000097:blood* / Q000378:metabolism; D016022:Case-Control Studies; D002853:Chromatography, Liquid; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood* / Q000378:metabolism; D037241:Mannose-Binding Lectins / Q000097:blood* / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000097:blood* / Q000378:metabolism; D040081:Protein Array Analysis; D060888:Real-Time Polymerase Chain Reaction; D053719:Tandem Mass Spectrometry
chemical_list: D037241:Mannose-Binding Lectins; D009363:Neoplasm Proteins