title: Chemical screening of an inhibitor for gibberellin receptors based on a yeast two-hybrid system.
abstract: We applied a yeast two-hybrid (Y2H) system to the high-throughput monitoring of two proteins' interaction, a receptor for phytohormone gibberellin (GA) and its direct signal transducer DELLA. With this system, we screened inhibitors to the interaction. As a result, we discovered a chemical, 3-(2-thienylsulfonyl)pyrazine-2-carbonitrile (TSPC), and we confirmed that TSPC is an inhibitor for GA perception by in vitro and in planta evaluations.
authors.affiliation: Department of Applied Biological Chemistry, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Jung-Min
authors.initials: JM
authors.lastname: Yoon
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Nakajima
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Mashiguchi
authors.forename: Seung-Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: Masato
authors.initials: M
authors.lastname: Otani
authors.forename: Tadao
authors.initials: T
authors.lastname: Asami
mesh_terms: D005875:Gibberellins / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D057166:High-Throughput Screening Assays; D010937:Plant Growth Regulators / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D030821:Plants, Genetically Modified; D011956:Receptors, Cell Surface / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques
chemical_list: D005875:Gibberellins; D010937:Plant Growth Regulators; D011956:Receptors, Cell Surface