title: Application of chiral ligand-exchange stationary phases in capillary electrochromatography.
abstract: The chiral separation principle ligand-exchange is applicable for HPLC, capillary electrophoresis as well as for capillary electrochromatography. Among other chiral separation principles, ligand-exchange permits simple method optimization and can be used particularly for chiral amino acids, amino alcohols or α-hydroxy acids. Up to now, three different approaches how to prepare capillaries for ligand-exchange electrochromatography have been published: The use of packed columns, monolithic columns, and particle-loaded columns. This chapter describes examples for the preparation of such columns for all three approaches and illustrates their use for chiral separations.
authors.affiliation: Institute of Pharmaceutical Sciences, Karl-Franzens-University, Graz, Austria. martin.schmid@uni-graz.at
authors.forename: Martin G
authors.initials: MG
authors.lastname: Schmid
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D000605:Amino Alcohols; D053801:Capillary Electrochromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods; D006880:Hydroxy Acids / Q000737:chemistry*; D008024:Ligands; D011108:Polymers / Q000737:chemistry; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D000605:Amino Alcohols; D006880:Hydroxy Acids; D008024:Ligands; D011108:Polymers