title: Dynamics of the DNA damage response: insights from live-cell imaging.
abstract: All organisms have to safeguard the integrity of their genome to prevent malfunctioning and oncogenic transformation. Sophisticated DNA damage response mechanisms have evolved to detect and repair genomic lesions. With the emergence of live-cell microscopy of individual cells, we now begin to appreciate the complex spatiotemporal kinetics of the DNA damage response and can address the causes and consequences of the heterogeneity in the responses of genetically identical cells. Here, we highlight key discoveries where live-cell imaging has provided unprecedented insights into how cells respond to DNA double-strand breaks and discuss the main challenges and promises in using this technique.
authors.affiliation: Department of Systems Biology, Harvard Medical School, Boston, MA, USA.
authors.forename: Ketki
authors.initials: K
authors.lastname: Karanam
authors.forename: Alexander
authors.initials: A
authors.lastname: Loewer
authors.forename: Galit
authors.initials: G
authors.lastname: Lahav
mesh_terms: D000818:Animals; D002470:Cell Survival; D004249:DNA Damage; D004260:DNA Repair; D006801:Humans; D021621:Imaging, Three-Dimensional; D059010:Single-Cell Analysis; D059008:Time-Lapse Imaging