title: 3D engineered cardiac tissue models of human heart disease: learning more from our mice.
abstract: Mouse engineered cardiac tissue constructs (mECTs) are a new tool available to study human forms of genetic heart disease within the laboratory. The cultured strips of cardiac cells generate physiologic calcium transients and twitch force, and respond to electrical pacing and adrenergic stimulation. The mECT can be made using cells from existing mouse models of cardiac disease, providing a robust readout of contractile performance and allowing a rapid assessment of genotype-phenotype correlations and responses to therapies. mECT represents an efficient and economical extension to the existing tools for studying cardiac physiology. Human ECTs generated from iPSCMs represent the next logical step for this technology and offer significant promise of an integrated, fully human, cardiac tissue model.
authors.affiliation: Department of Pediatrics, University of Wisconsin School of Medicine and Public Health, Madison, WI 53792, USA. jcralphe@pediatrics.wisc.edu
authors.forename: J Carter
authors.initials: JC
authors.lastname: Ralphe
authors.forename: Willem J
authors.initials: WJ
authors.lastname: de Lange
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006321:Heart / Q000503:physiopathology*; D006331:Heart Diseases / Q000235:genetics / Q000503:physiopathology*; D006801:Humans; D021621:Imaging, Three-Dimensional; D051379:Mice; D023822:Tissue Engineering / Q000379:methods*