title: Transient receptor potential vanilloid 2 (TRPV2), a potential novel biomarker in childhood asthma.
abstract: BACKGROUND
  The presence of transient receptor potential vanilloid 2 (TRPV2) in human peripheral blood cells may suggest a role under pathological conditions. The aim of this study was to explore the relationship between the expression profile of TRPV2 gene and childhood asthma in the north of China. The effects of allergens exposure on the expression of TRPV2 gene were also investigated.
  
  
  METHODS
  Sixty asthmatics children confirmed by physician diagnosis and 60 healthy children as a control group were recruited. Serum total IgE and specific IgE were measured. Using quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR), TRPV2 was detected in total RNA extracted from peripheral blood lymphocytes. Student's t-test and chi-square test were used to analyze the relationship between TRPV2 transcript and different parameter variables on susceptibility of childhood asthma. Multiple logistic regression was used to analyze the associations between TRPV2 gene and allergens.
  
  
  RESULTS
  The expression level of TRPV2 gene was increased 2.6 times in asthmatic children compared with controls (p < .01). The up-regulation of TRPV2 gene and sensitization to one of three the allergens-spring pollen, dust mite, and dog and cat hair-were correlated with childhood asthma. In addition, the hypersensitivity to spring pollen, cockroach, and dust mite and up-regulation of TRPV2 gene expression may be the risk factors for the childhood asthma in Beijing.
  
  
  CONCLUSIONS
  The increased expression of TRPV2 gene in peripheral lymphocytes is closely correlated with childhood asthma in the north of China. This study provides a potential new biomarker of childhood asthma and lays the basis for further clarification of the pathogenesis underlying asthma.
authors.affiliation: Laboratory of Molecular Genetics, Beijing Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Cai
authors.forename: Yong-chang
authors.initials: YC
authors.lastname: Yang
authors.forename: Jing-feng
authors.initials: JF
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.forename: Wen-liang
authors.initials: WL
authors.lastname: Fu
authors.forename: Ze-yi
authors.initials: ZY
authors.lastname: Zhu
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Wang
authors.forename: Min-ji
authors.initials: MJ
authors.lastname: Zou
authors.forename: Jia-xi
authors.initials: JX
authors.lastname: Wang
authors.forename: Dong-qun
authors.initials: DQ
authors.lastname: Xu
authors.forename: Dong-gang
authors.initials: DG
authors.lastname: Xu
mesh_terms: D000485:Allergens / Q000276:immunology; D001249:Asthma / Q000097:blood / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D002681:China; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D016015:Logistic Models; D008297:Male; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050916:TRPV Cation Channels / Q000097:blood / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014505:Urban Population
chemical_list: D000485:Allergens; D015415:Biomarkers; D050916:TRPV Cation Channels; C400769:TRPV2 protein, human; D007073:Immunoglobulin E; D012313:RNA