title: Therapeutic effect of zinc-containing calcium phosphate suspension injection in thermal burn-rats.
abstract: The aim of this study was to evaluate the efficacy of suspensions of zinc-containing tricalcium phosphate (TCP) in the healing of thermal burns in rats. β-ZnTCP containing 10 mol % zinc, α-ZnTCP containing 0.9 mol % zinc, and ZnSO4 ·(H2O)7 (ZnSO4) were used. The injections were prepared to suspend ZnSO4 , α-ZnTCP, and β-ZnTCP powders in 2 mL of 1% sodium alginate saline solution containing 2 mg of Zn. In vitro Zn release rates were measured in simulated body fluid. The release of Zn from ZnSO4 was very fast, but that from α-ZnTCP and β-ZnTCP was slowed by transformation to hydroxyapatite. The suspensions were injected into group C (control), D1 (ZnSO4), D2 (α-ZnTCP), and D3 (β-ZnTCP) rats after thermal burns treatment for 3 h. The area under the curve for the plasma Zn for group D1 was the highest, and the order was groups D1 > D2 ≥ D3 ≥ C. The wounded area (Aw) of group D1 had almost the same profile as that of group C, and the Aw at 18 days was about 20%. In contrast, the Aw of group D2 and D3 decreased, and on day 15 was 8% and 37%, respectively. The results indicated that the healing process was shorter in the rats given α-ZnTCP and β-ZnTCP than those given ZnSO4 or the control.
authors.affiliation: Research Institute of Pharmaceutical Sciences, Faculty of Pharmacy, Musashino University, Tokyo, Japan. motsuka@musashino-u.ac.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Otsuka
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shikamura
authors.forename: Kuniko
authors.initials: K
authors.lastname: Otsuka
authors.forename: Yu
authors.initials: Y
authors.lastname: Sogo
authors.forename: Atsuo
authors.initials: A
authors.lastname: Ito
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D002056:Burns / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D002130:Calcium Phosphates / Q000737:chemistry*; D007267:Injections; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000187:drug effects; D015032:Zinc / Q000008:administration & dosage* / Q000097:blood / Q000737:chemistry / Q000627:therapeutic use*
chemical_list: D002130:Calcium Phosphates; D000469:Alkaline Phosphatase; D015032:Zinc; C018392:tricalcium phosphate