title: Programming human dendritic cells with mRNA.
abstract: Transfecting with in vitro transcribed, protein-encoding mRNA is a simple yet effective method to express high levels of the desired RNA-encoded proteins in primary cells. Cells can be transfected with antigen-encoding mRNA, which is translated into protein and is processed by the cellular antigen-processing pathway to generate antigen-presenting cells. Another elegant and increasingly popular application is to transfect cells with mRNA that encodes immune modulating molecules (cytokines, chemokines, toll-like receptors (TLRs), immune receptor ligands, immune receptor targeting antibodies) which, when translated into protein, can program cell behavior and/or function. In this chapter we describe an efficient method to deliver mRNA into human dendritic cells (DCs) by electroporation. This is currently the method of choice to deliver mRNA into antigen-presenting cells for generating vaccines for cancer immunotherapy.
authors.affiliation: Department of Surgery, Duke University Medical Center, Durham, NC, USA.
authors.forename: Jaewoo
authors.initials: J
authors.lastname: Lee
authors.forename: David
authors.initials: D
authors.lastname: Boczkowski
authors.forename: Smita
authors.initials: S
authors.lastname: Nair
mesh_terms: D000941:Antigens / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D019496:Cancer Vaccines / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D060846:Cell Engineering / Q000379:methods*; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D009369:Neoplasms / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000628:therapy; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D051193:Toll-Like Receptors / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D014162:Transfection / Q000379:methods*
chemical_list: D000941:Antigens; D019496:Cancer Vaccines; D016207:Cytokines; D012333:RNA, Messenger; D051193:Toll-Like Receptors