title: Biodegradable and bioactive porous scaffold structures prepared using fused deposition modeling.
abstract: Three-dimensional printing (3DP) refers to a group of additive manufacturing techniques that can be utilized in tissue engineering applications. Fused deposition modeling (FDM) is a 3DP method capable of using common thermoplastic polymers. However, the scope of materials applicable for FDM has not been fully recognized. The purpose of this study was to examine the creation of biodegradable porous scaffold structures using different materials in FDM and to determine the compressive properties and the fibroblast cell response of the structures. To the best of our knowledge, the printability of a poly(ε-caprolactone)/bioactive glass (PCL/BAG) composite and L-lactide/ε-caprolactone 75/25 mol % copolymer (PLC) was demonstrated for the first time. Scanning electron microscope (SEM) images showed BAG particles at the surface of the printed PCL/BAG scaffolds. Compressive testing showed the possibility of altering the compressive stiffness of a scaffold without changing the compressive modulus. Compressive properties were significantly dependent on porosity level and structural geometry. Fibroblast proliferation was significantly higher in polylactide than in PCL or PCL/BAG composite. Optical microscope images and SEM images showed the viability of the cells, which demonstrated the biocompatibility of the structures.
authors.affiliation: Polymer Technology Research Group, School of Chemical Technology, Aalto University, Aalto 00076, Finland.
authors.forename: Jyrki
authors.initials: J
authors.lastname: Korpela
authors.forename: Anne
authors.initials: A
authors.lastname: Kokkari
authors.forename: Harri
authors.initials: H
authors.lastname: Korhonen
authors.forename: Minna
authors.initials: M
authors.lastname: Malin
authors.forename: Timo
authors.initials: T
authors.lastname: Närhi
authors.forename: Jukka
authors.initials: J
authors.lastname: Seppälä
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D001842:Bone and Bones / Q000473:pathology*; D049109:Cell Proliferation; D002470:Cell Survival; D019245:Compressive Strength; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005881:Gingiva / Q000166:cytology; D006801:Humans; D021621:Imaging, Three-Dimensional; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C016240:polycaprolactone