title: Analysis of exopolysaccharides in Myxococcus xanthus using confocal laser scanning microscopy.
abstract: Confocal laser scanning microscopy (CLSM) is a useful research tool to explore the exopolysaccharides (EPS) in bacterial biofilms. Here, we describe the analysis of different biofilms of Myxococcus xanthusformed in a modified chamber slide system with CLSM. In conjunction with several specific fluorescent probes, the EPS within M. xanthusbiofilms can be visualized and analyzed in situ.
authors.affiliation: School of Dentistry, University of California, Los Angeles, CA, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.forename: Renate
authors.initials: R
authors.lastname: Lux
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Shi
mesh_terms: D018441:Biofilms; D018613:Microscopy, Confocal / Q000379:methods*; D016941:Myxococcus xanthus / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis*
chemical_list: D011134:Polysaccharides