title: Post-translational regulation of a developmental catalase, CatB, involves a metalloprotease, SmpA and contributes to proper differentiation and osmoprotection of Streptomyces coelicolor.
abstract: Streptomyces coelicolor produces at least three different catalases (catalases A, B, and C) under different physiological conditions. Catalase B (CatB) is a developmentally regulated catalase required for proper differentiation and osmoprotection of S. coelicolor. We previously observed that the N-terminal 75(2) amino acids (aa) of CatB are cleaved off, with the remaining 75-kDa processed CatB detectable in the extracellular fraction during sporulation. We here report that either the deletion of the N-terminal 75 aa or the arginine-to-alanine substitution (R75A) at the cleavage site, but not the histidine-to-alanine substitution (H131A) at the catalytic site, impaired both the secretion of CatB proteins and the proper differentiation of S. coelicolor cells. The proteolytic activity responsible for the cleavage of CatB was purified and then identified as a metalloprotease, which was named as SmpA (Streptomyces metalloprotease A). The SmpA protein was newly detected after sporulation, coincident with the intracellular appearance of 75-kDa CatB, which was not detected in the smpA null mutant, confirming that SmpA indeed processes CatB in vivo. The smpA mutant was osmosensitive as catB mutant, but it displayed delayed sporulation, with the 75-kDa CatB still detectable in the extracellular milieu. Based on these results, we propose that the post-translational regulation of CatB, which cleaves the N-terminal 75 aa residues through SmpA is crucial for proper differentiation and osmoprotection of S. coelicolor. In the absence of SmpA, an alternative route for CatB processing may function to allow delayed sporulation.
authors.affiliation: School of Biological Sciences, Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Hyo-Sub
authors.initials: HS
authors.lastname: Kim
authors.forename: Eun-Jin
authors.initials: EJ
authors.lastname: Lee
authors.forename: You-Hee
authors.initials: YH
authors.lastname: Cho
authors.forename: Jung-Hye
authors.initials: JH
authors.lastname: Roe
mesh_terms: D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D020134:Catalytic Domain; D004252:DNA Mutational Analysis; D015964:Gene Expression Regulation, Bacterial; D045726:Metalloproteases / Q000378:metabolism*; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D009997:Osmotic Pressure; D017354:Point Mutation; D011499:Protein Processing, Post-Translational; D017384:Sequence Deletion; D048372:Streptomyces coelicolor / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D013312:Stress, Physiological
chemical_list: D050505:Mutant Proteins; D002374:Catalase; D045726:Metalloproteases