title: Chemiluminescent detection of senescence-associated β galactosidase.
abstract: Identifying molecules that serve as markers for cell aging is a goal that has been pursued by several groups. Senescence-associated β galactosidase (SA-βgal) staining is broadly used and very easily detected. β-gal is a lysosomal enzyme strongly correlated to the progression of cell senescence. Here, we describe a simple, fast, and quantitative protocol to quantify SA-βgal activity in cell lysate extracts by a chemiluminescent method using galacton as substrate.
authors.affiliation: Laboratory of Genetics and Molecular Cardiology/LIM 13, Heart Institute (InCor), University of Sao Paulo Medical School, Sao Paulo, Brazil.
authors.forename: Vinicius
authors.initials: V
authors.lastname: Bassaneze
authors.forename: Ayumi Aurea
authors.initials: AA
authors.lastname: Miyakawa
authors.forename: José Eduardo
authors.initials: JE
authors.lastname: Krieger
mesh_terms: D016922:Cellular Senescence; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000201:enzymology; D013997:Time Factors; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D001616:beta-Galactosidase