title: Analysis of natural variation in bermudagrass (Cynodon dactylon) reveals physiological responses underlying drought tolerance.
abstract: Bermudagrass (Cynodon dactylon) is a widely used warm-season turfgrass and one of the most drought tolerant species. Dissecting the natural variation in drought tolerance and physiological responses will bring us powerful basis and novel insight for plant breeding. In the present study, we evaluated the natural variation of drought tolerance among nine bermudagrass varieties by measuring physiological responses after drought stress treatment through withholding water. Three groups differing in drought tolerance were identified, including two tolerant, five moderately tolerant and two susceptible varieties. Under drought stress condition, drought sensitive variety (Yukon) showed relative higher water loss, more severe cell membrane damage (EL), and more accumulation of hydrogen peroxide (H₂O₂) and malondialdehyde (MDA), while drought tolerant variety (Tifgreen) exhibited significantly higher antioxidant enzymes activities. Further results indicated that drought induced cell injury in different varieties (Yukon, SR9554 and Tifgreen) exhibited liner correlation with leaf water content (LWC), H₂O₂ content, MDA content and antioxidant enzyme activities. Additionally, Tifgreen plants had significantly higher levels of osmolytes (proline level and soluble sugars) when compared with Yukon and SR9554 under drought stress condition. Taken together, our results indicated that natural variation of drought stress tolerance in bermudagrass varieties might be largely related to the induced changes of water status, osmolyte accumulation and antioxidant defense system.
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Shi
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhangmin
authors.initials: Z
authors.lastname: Cheng
authors.forename: Tiantian
authors.initials: T
authors.lastname: Ye
authors.forename: Zhulong
authors.initials: Z
authors.lastname: Chan
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D000975:Antioxidants / Q000378:metabolism; D002241:Carbohydrates / Q000032:analysis; D031744:Cynodon / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D003681:Dehydration / Q000235:genetics*; D055864:Droughts; D014644:Genetic Variation / Q000502:physiology*; D011392:Proline / Q000032:analysis; D017382:Reactive Oxygen Species / Q000378:metabolism; D013045:Species Specificity; D013312:Stress, Physiological / Q000235:genetics; D014882:Water-Electrolyte Balance / Q000235:genetics; D015874:Yukon Territory
chemical_list: D000975:Antioxidants; D002241:Carbohydrates; D017382:Reactive Oxygen Species; D011392:Proline