title: Dehydroglyasperin C, a component of liquorice, attenuates proliferation and migration induced by platelet-derived growth factor in human arterial smooth muscle cells.
abstract: Liquorice is one of the botanicals used frequently as a traditional medicine in the West and in the East. Platelet-derived growth factor (PDGF)-BB is involved in the development of CVD by inducing abnormal proliferation and migration of vascular smooth muscle cells. In our preliminary study, dehydroglyasperin C (DGC), an active compound of liquorice, showed strong antioxidant activity. Since phytochemicals with antioxidant activities showed beneficial effects on chronic inflammatory diseases, the present study aimed to investigate the effects of DGC on PDGF-induced proliferation and migration of human aortic smooth muscle cells (HASMC). Treatment of HASMC with DGC for 24 h significantly decreased PDGF-induced cell number and DNA synthesis in a dose-dependent manner without any cytotoxicity, as demonstrated by the 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide test and thymidine incorporation. Upon cell cycle analysis, DGC blocked the PDGF-induced progression through the G0/G1 to S phase of the cell cycle, and down-regulated the expression of cyclin-dependent kinase (CDK); 2, cyclin E, CDK4 and cyclin D1. Furthermore, DGC significantly attenuated PDGF-stimulated phosphorylation of PDGF receptor-b, phospholipase C-g1, AKT and extracellular-regulated kinase 1/2, and DGC inhibited cell migration and the dissociation of actin filaments by PDGF. In a rat vascular balloon injury model, DGC suppressed an excessive reduction in luminal diameters and neointimal formation compared with the control group. These results demonstrate the mechanistic basis for the prevention of CVD and the potential therapeutic properties of DGC.
authors.affiliation: School of Applied Biosciences, Food Science and Biotechnology, BK21 Research Team for Developing Functional Health Food Materials, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Hyo Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Byung-Yoon
authors.initials: BY
authors.lastname: Cha
authors.forename: In Sil
authors.initials: IS
authors.lastname: Park
authors.forename: Ji Sun
authors.initials: JS
authors.lastname: Lim
authors.forename: Je-Tae
authors.initials: JT
authors.lastname: Woo
authors.forename: Jong-Sang
authors.initials: JS
authors.lastname: Kim
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use; D001011:Aorta / Q000187:drug effects; D001578:Benzopyrans / Q000494:pharmacology* / Q000627:therapeutic use; D002453:Cell Cycle / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D016213:Cyclins / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D015536:Down-Regulation; D006035:Glycyrrhiza / Q000737:chemistry*; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010766:Phosphorylation; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D058226:Plaque, Atherosclerotic / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D051381:Rats
chemical_list: D000975:Antioxidants; D001578:Benzopyrans; D016213:Cyclins; D010936:Plant Extracts; D010982:Platelet-Derived Growth Factor; C548688:dehydroglyasperin C; D004247:DNA