title: Pleionea mediterranea gen. nov., sp. nov., a gammaproteobacterium isolated from coastal seawater.
abstract: A Gram-negative, aerobic, cream-pigmented, non-motile, non-spore-forming straight rod, strain MOLA115(T), was isolated from a coastal water sample from the Mediterranean Sea. On the basis of phylogenetic analysis of the 16S rRNA gene sequences, strain MOLA115(T) was shown to belong to the Gammaproteobacteria, adjacent to members of the genera Marinicella, Arenicella and Kangiella, sharing less than 89 % 16S rRNA gene sequence similarity with strains of all recognized species within the Gammaproteobacteria. The only isoprenoid quinone was ubiquinone-8. Polar lipids in strain MOLA115(T) included phosphatidylethanolamine, an aminolipid, phosphatidylglycerol and an aminophospholipid. Fatty acid analysis revealed iso-C15 : 0 and iso-C17 : 1ω9c to be the dominant components. The DNA G+C content was 44.5 mol%. Based upon the phenotypic and phylogenetic data, we propose that strain MOLA115(T) should be considered to represent a novel species in a new genus, for which the name Pleionea mediterranea gen. nov., sp. nov. is proposed. The type strain of Pleionea mediterranea is MOLA115(T) ( = CIP 110343(T) = DSM 25350(T)).
authors.affiliation: CNRS, UMR 7621, LOMIC, Observatoire Océanologique, F-66650, Banyuls/Mer, France.|UPMC Université Paris 6, UMS 2348, UMR 7621, Observatoire Océanologique, F-66650 Banyuls/Mer, France.
authors.forename: Sonja K
authors.initials: SK
authors.lastname: Fagervold
authors.affiliation: Université de Pau et des Pays de l'Adour, IPREM UMR 5254, Equipe Environnement et Microbiologie, IBEAS, F-64013 PAU, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Urios
authors.affiliation: CNRS, UMS 2348, Observatoire Océanologique, F-66650, Banyuls/Mer, France.|UPMC Université Paris 6, UMS 2348, UMR 7621, Observatoire Océanologique, F-66650 Banyuls/Mer, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Intertaglia
authors.affiliation: CNRS, UMR 7621, LOMIC, Observatoire Océanologique, F-66650, Banyuls/Mer, France.|UPMC Université Paris 6, UMS 2348, UMR 7621, Observatoire Océanologique, F-66650 Banyuls/Mer, France.
authors.forename: Nicole
authors.initials: N
authors.lastname: Batailler
authors.affiliation: CNRS, UMR 7621, LOMIC, Observatoire Océanologique, F-66650, Banyuls/Mer, France.|UPMC Université Paris 6, UMS 2348, UMR 7621, Observatoire Océanologique, F-66650 Banyuls/Mer, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Lebaron
authors.affiliation: CNRS, UMR 7621, LOMIC, Observatoire Océanologique, F-66650, Banyuls/Mer, France.|UPMC Université Paris 6, UMS 2348, UMR 7621, Observatoire Océanologique, F-66650 Banyuls/Mer, France.
authors.forename: Marcelino T
authors.initials: MT
authors.lastname: Suzuki
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008522:Mediterranean Sea; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8