title: Effects of hanging drop culture conditions on embryoid body formation and neuronal cell differentiation using mouse embryonic stem cells: optimization of culture conditions for the formation of well-controlled embryoid bodies.
abstract: Hanging drop (HD) cultures were carried out with a drop volume of either 20 or 30 μl. An incubation period of 3 days was determined to be appropriate for the formation of well-controlled embryoid bodies (EBs), and the initial cell number was identified as the most critical factor in the growth and neuronal cell differentiation of EBs.
authors.affiliation: Division of Medicine and Engineering Science, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi 400-8511, Japan.
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Ohnuki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kurosawa
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D058732:Embryoid Bodies / Q000166:cytology*; D053595:Embryonic Stem Cells / Q000166:cytology; D051379:Mice; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology*