title: Crystallization and preliminary X-ray diffraction studies of Drosophila melanogaster Gαo-subunit of heterotrimeric G protein in complex with the RGS domain of CG5036.
abstract: Regulator of G-protein signalling (RGS) proteins negatively regulate heterotrimeric G-protein signalling through their conserved RGS domains. RGS domains act as GTPase-activating proteins, accelerating the GTP hydrolysis rate of the activated form of Gα-subunits. Although omnipresent in eukaryotes, RGS proteins have not been adequately analysed in non-mammalian organisms. The Drosophila melanogaster Gαo-subunit and the RGS domain of its interacting partner CG5036 have been overproduced and purified; the crystallization of the complex of the two proteins using PEG 4000 as a crystallizing agent and preliminary X-ray crystallographic analysis are reported. Diffraction data were collected to 2.0 Å resolution using a synchrotron-radiation source.
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russian Federation. sveta@vega.protres.ru
authors.forename: Svetlana
authors.initials: S
authors.lastname: Tishchenko
authors.forename: Azat
authors.initials: A
authors.lastname: Gabdulkhakov
authors.forename: Uliana
authors.initials: U
authors.lastname: Tin
authors.forename: Olga
authors.initials: O
authors.lastname: Kostareva
authors.forename: Chen
authors.initials: C
authors.lastname: Lin
authors.forename: Vladimir L
authors.initials: VL
authors.lastname: Katanaev
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray; D029721:Drosophila Proteins / Q000737:chemistry* / Q000378:metabolism*; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D011092:Polyethylene Glycols / Q000737:chemistry; D017434:Protein Structure, Tertiary; D020710:RGS Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D020710:RGS Proteins; C582291:dhit protein, Drosophila; D011092:Polyethylene Glycols; D019206:GTP-Binding Protein alpha Subunits, Gi-Go