title: Comprehensive evaluation of two HLA-B17 monoclonal antibodies for flow cytometry-based HLA-B57/B58 screening prior to abacavir prescription.
abstract: Hypersensitivity reactions to the drug abacavir, used to treat HIV/AIDS patients, is associated with possession of HLA-B*57:01. We have carefully assessed two commercially available HLA-B57/B58 murine monoclonal antibodies [0196HA and BIH0243 (One Lambda Inc.)] in a simple flow cytometry-based assay. The evaluation involved tests on 228 reference and random samples covering 91% of all WHO recognized HLA-A, B and C specificities. These involved donors with six different HLA-B*57 alleles and included 19 examples of B*57:01. Both antibodies unambiguously detected B57, but there were small difference in their reactivity against B57-positive non-B*57:01 samples. Importantly, there was no reactivity against B57/B58-negative samples. The possible amino acid motifs involved in the reactivity of these antibodies with B57/B58 were delineated. Thus, HLA-B57/B58, normally present in <10% of patients, can be easily recognized using these two antibodies and further tested by a DNA-based typing method to identify B*57:01.
authors.affiliation: Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Wales, UK.
authors.forename: R
authors.initials: R
authors.lastname: Stevens
authors.forename: E
authors.initials: E
authors.lastname: Coates
authors.forename: J
authors.initials: J
authors.lastname: Street
authors.forename: E
authors.initials: E
authors.lastname: Cook
authors.forename: C
authors.initials: C
authors.lastname: Darke
mesh_terms: D000483:Alleles; D019380:Anti-HIV Agents / Q000009:adverse effects* / Q000627:therapeutic use; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015224:Dideoxynucleosides / Q000009:adverse effects* / Q000627:therapeutic use; D004342:Drug Hypersensitivity / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*; D011307:Drug Prescriptions; D000939:Epitopes / Q000235:genetics; D005434:Flow Cytometry; D014644:Genetic Variation; D015235:HLA-B Antigens / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D006801:Humans; D000099227:Abacavir
chemical_list: D019380:Anti-HIV Agents; D000911:Antibodies, Monoclonal; D015224:Dideoxynucleosides; D000939:Epitopes; D015235:HLA-B Antigens; D000099227:Abacavir; C484830:HLA-B*57:01 antigen; C037449:HLA-B17 antigen; C041533:HLA-B58 antigen