title: Evaluation of three selective chromogenic media, CHROMagar ESBL, CHROMagar CTX-M and CHROMagar KPC, for the detection of Klebsiella pneumoniae producing OXA-48 carbapenemase.
abstract: Three selective chromogenic culture media (CHROMagars ESBL, CTX-M and KPC) were evaluated for their ability to support the growth of nine Klebsiella pneumoniae isolates producing OXA-48 carbapenemase in combination with other β-lactamases. CHROMagar ESBL and CHROMagar KPC were the most sensitive media, supporting growth of all isolates with a detection limit as low as < 100 CFU/ml. Five isolates failed to grow on CHROMagar CTX-M, and five were recovered on CHROMagar KPC only at counts > 10(6) CFU/ml. Both CHROMagar ESBL and CHROMagar KPC may be useful for enhanced isolation of K pneumoniae producing OXA-48-like carbapenemases.
authors.affiliation: Antimicrobial Research Group, Centre for Immunology and Infectious Disease, Blizard Institute, Barts & The London School of Medicine and Dentistry, Queen Mary University London, London, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Hornsey
authors.forename: Lynette
authors.initials: L
authors.lastname: Phee
authors.forename: Neil
authors.initials: N
authors.lastname: Woodford
authors.forename: Jane
authors.initials: J
authors.lastname: Turton
authors.forename: Daniele
authors.initials: D
authors.lastname: Meunier
authors.forename: Claire
authors.initials: C
authors.lastname: Thomas
authors.forename: David W
authors.initials: DW
authors.lastname: Wareham
mesh_terms: D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism*; D001431:Bacteriological Techniques; D002863:Chromogenic Compounds; D015169:Colony Count, Microbial; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D057230:Limit of Detection; D008826:Microbial Sensitivity Tests; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002863:Chromogenic Compounds; D000362:Agar; D001618:beta-Lactamases; C063912:carbapenemase; C108449:oxacillinase