title: Candida albicans stimulates in vivo differentiation of haematopoietic stem and progenitor cells towards macrophages by a TLR2-dependent signalling.
abstract: Toll-like receptors (TLRs) are expressed by haematopoietic stem and progenitor cells (HSPCs), and may play a role in haematopoiesis in response to pathogens during infection. We have previously demonstrated that (i) inactivated yeasts of Candida albicans induce in vitro differentiation of HSPCs towards the myeloid lineage, and (ii) soluble TLR agonists induce in vivo their differentiation towards macrophages. In this work, using an in vivo model of HSPCs transplantation, we report for the first time that HSPCs sense C. albicans in vivo and subsequently are directed to produce macrophages by a TLR2-dependent signalling. Purified lineage-negative cells (Lin(-)) from bone marrow of C57BL/6 mice (CD45.2 alloantigen) were transplanted into B6Ly5.1 mice (CD45.1 alloantigen), which were then injected with viable or inactivated C. albicans yeasts. Transplanted cells were detected in the spleen and in the bone marrow of recipient mice, and they differentiate preferentially to macrophages, both in response to infection or in response to inactivated yeasts. The generation of macrophages was dependent on TLR2 but independent of TLR4, as transplanted Lin(-) cells from TLR2(-/-) mice did not give rise to macrophages, whereas Lin(-) cells from TLR4(-/-) mice generated macrophages similarly to control cells. Interestingly, the absence of TLR2, or in a minor extent TLR4, gives Lin(-) cells an advantage in transplantation assays, as increases the percentage of transplanted recovered cells. Our results indicatethat TLR-mediated recognition of C. albicans by HSPCs may help replace and/or increase cells that constitute the first line of defence against the fungus, and suggest that TLR-mediated signalling may lead to reprogramming early progenitors to rapidly replenishing the innate immune system and generate the most necessary mature cells to deal with the pathogen.
authors.affiliation: Departamento de Microbiología y Ecología, Universitat de València, Burjassot, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Megías
authors.forename: Victoria
authors.initials: V
authors.lastname: Maneu
authors.forename: Pedro
authors.initials: P
authors.lastname: Salvador
authors.forename: Daniel
authors.initials: D
authors.lastname: Gozalbo
authors.forename: M Luisa
authors.initials: ML
authors.lastname: Gil
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000276:immunology / Q000382:microbiology; D002176:Candida albicans / Q000276:immunology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D006412:Hematopoietic Stem Cells / Q000382:microbiology / Q000502:physiology*; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D023421:Models, Animal; D015398:Signal Transduction; D013154:Spleen / Q000276:immunology / Q000382:microbiology; D051195:Toll-Like Receptor 2 / Q000378:metabolism*
chemical_list: C493486:Tlr2 protein, mouse; D051195:Toll-Like Receptor 2