title: Soy milk as a storage medium to preserve human fibroblast cell viability: an in vitro study.
abstract: Soy milk (SM) is widely consumed worldwide as a substitute for cow milk. It is a source of vitamins, carbohydrates and sugars, but its capacity to preserve cell viability has not been evaluated. The purpose of the present study was to investigate the efficacy of SM to maintain the viability of human fibroblasts at short periods compared with different cow milks. Human mouth fibroblasts were cultured and stored in the following media at room temperature: 10% Dulbecco's Modified Eagle Medium (DMEM) (positive control group); long shelf-life ultra-high temperature whole cow milk (WM); long shelf-life ultra-high temperature skim cow milk (SKM); powdered cow milk (PM); and soy milk (SM). After 5, 15, 30 and 45 min, cell viability was analyzed using the MTT assay. Data were analyzed statistically by the Kruskal-Wallis test with post-analysis using the Dunn's method (α=0.05). SKM showed the lowest capacity to maintain cell viability in all analyzed times (p<0.05). At 30 and 45 min, the absorbance levels in control group (DMEM) and SM were significantly higher than in SKM (p<0.05). Cell viability decreased along the time (5-45 min). The results indicate that SM can be used as a more adequate storage medium for avulsed teeth. SKM was not as effective in preserving cell viability as the cell culture medium and SM.
authors.affiliation: Department of Operative Dentistry and Dental Materials, Dental School, Federal University of Uberlândia, MG, Brazil.
authors.forename: Camilla Christian Gomes
authors.initials: CC
authors.lastname: Moura
authors.forename: Priscilla Barbosa Ferreira
authors.initials: PB
authors.lastname: Soares
authors.forename: Manuella Verdinelli de Paula
authors.initials: MV
authors.lastname: Reis
authors.forename: Alfredo Júlio
authors.initials: AJ
authors.lastname: Fernandes Neto
authors.forename: Carlos José
authors.initials: CJ
authors.lastname: Soares
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002470:Cell Survival; D003470:Culture Media; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D008892:Milk; D019188:Organ Preservation Solutions; D010513:Periodontal Ligament / Q000166:cytology*; D041942:Soy Milk
chemical_list: D003470:Culture Media; D019188:Organ Preservation Solutions