title: Molecular characterization of neoplastic and normal "sister" lymphoblastoid B-cell lines from chronic lymphocytic leukemia.
abstract: Chronic lymphocytic leukemia (CLL) B-cells resemble self-renewing CD5 + B-cells carrying auto/xeno-antigen-reactive B-cell receptors (BCRs) and multiple innate pattern-recognition receptors, such as Toll-like receptors and scavenger receptors. Integration of signals from BCRs with multiple surface membrane receptors determines whether the cells will be proliferating, anergic or apoptotic. To better understand the role of antigen in leukemogenesis, CLL cell lines producing monoclonal antibodies (mAbs) will facilitate structural analysis of antigens and supply DNA for genetic studies. We present here a comprehensive genotypic and phenotypic characterization of available CLL and normal B-cell-derived lymphoblastoid cell lines (LCLs) from the same individuals (n = 17). Authenticity and verification studies of CLL-patient origin were done by IGHV sequencing, fluorescence in situ hybridization (FISH) and DNA/short tandem repeat (STR) fingerprinting. Innate B-cell features, i.e. natural Ab production and CD5 receptors, were present in most CLL cell lines, but in none of the normal LCLs. This panel of immortalized CLL-derived cell lines is a valuable reference representing a renewable source of authentic Abs and DNA.
authors.affiliation: Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Lanemo Myhrinder
authors.forename: Eva
authors.initials: E
authors.lastname: Hellqvist
authors.forename: Ann-Charlotte
authors.initials: AC
authors.lastname: Bergh
authors.forename: Mattias
authors.initials: M
authors.lastname: Jansson
authors.forename: Kenneth
authors.initials: K
authors.lastname: Nilsson
authors.forename: Per
authors.initials: P
authors.lastname: Hultman
authors.forename: Jon
authors.initials: J
authors.lastname: Jonasson
authors.forename: Anne Mette
authors.initials: AM
authors.lastname: Buhl
authors.forename: Lone
authors.initials: L
authors.lastname: Bredo Pedersen
authors.forename: Jesper
authors.initials: J
authors.lastname: Jurlander
authors.forename: Eva
authors.initials: E
authors.lastname: Klein
authors.forename: Nicole
authors.initials: N
authors.lastname: Weit
authors.forename: Marco
authors.initials: M
authors.lastname: Herling
authors.forename: Richard
authors.initials: R
authors.lastname: Rosenquist
authors.forename: Anders
authors.initials: A
authors.lastname: Rosén
mesh_terms: D000368:Aged; D000595:Amino Acid Sequence; D000954:Antigens, Surface / Q000378:metabolism; D001402:B-Lymphocytes / Q000378:metabolism; D045744:Cell Line, Tumor; D022801:Complementarity Determining Regions / Q000235:genetics; D005260:Female; D015973:Gene Expression Regulation, Leukemic; D015322:Gene Rearrangement, B-Lymphocyte; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D006801:Humans; D016130:Immunophenotyping; D059785:Karyotype; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000378:metabolism*; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D008969:Molecular Sequence Data; D010641:Phenotype; D011947:Receptors, Antigen, B-Cell / Q000235:genetics; D059467:Transcriptome
chemical_list: D000954:Antigens, Surface; D022801:Complementarity Determining Regions; D011947:Receptors, Antigen, B-Cell