title: Integrin upregulation and localization to focal adhesion sites in pregnant human myometrium.
abstract: Focal adhesions are integrin-rich microdomains that structurally link the cytoskeleton to the extracellular matrix and transmit mechanical signals. In the pregnant uterus, increases in integrin expression and activation are thought to be critical for the formation of the mechanical syncytium required for labor. The aim of this study was to determine which integrins are upregulated and localized to focal adhesions in pregnant human myometrium. We used quantitative polymerase chain reaction, Western blotting, and confocal microscopy to determine the expression levels and colocalization with focal adhesion proteins. We observed increases in several integrin transcripts in pregnant myometrium. At the protein level, integrins such as α5-integrin (ITGA5), ITGA7, ITGAV, and ITGB3 were significantly increased during pregnancy. The integrins ITGA3, ITGA5, ITGA7, and ITGB1 colocalized with focal adhesion proteins in term human myometrium. These data suggest that integrins α3β1, α5β1, and α7β1 are the most likely candidates to transmit mechanical signals from the extracellular matrix through focal adhesions in pregnant human myometrium.
authors.affiliation: Department of Pharmacology, University of Nevada School of Medicine, Center for Molecular Medicine, Reno, NV 89557, USA.
authors.forename: Heather R
authors.initials: HR
authors.lastname: Burkin
authors.forename: Monica
authors.initials: M
authors.lastname: Rice
authors.forename: Apurva
authors.initials: A
authors.lastname: Sarathy
authors.forename: Sara
authors.initials: S
authors.lastname: Thompson
authors.forename: Cherie A
authors.initials: CA
authors.lastname: Singer
authors.forename: Iain L O
authors.initials: IL
authors.lastname: Buxton
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D022001:Focal Adhesions / Q000378:metabolism*; D006801:Humans; D039001:Integrin alpha Chains / Q000096:biosynthesis*; D039641:Integrin beta Chains / Q000096:biosynthesis*; D008875:Middle Aged; D009215:Myometrium / Q000378:metabolism*; D011247:Pregnancy / Q000378:metabolism*; D015854:Up-Regulation / Q000502:physiology*; D055815:Young Adult
chemical_list: D039001:Integrin alpha Chains; D039641:Integrin beta Chains
keywords: integrin; myometrium; pregnancy