title: Anchoring ethinylestradiol induced gene expression changes with testicular morphology and reproductive function in the medaka.
abstract: Environmental estrogens are ubiquitous in the environment and can cause detrimental effects on male reproduction. In fish, a multitude of effects from environmental estrogens have been observed including altered courting behavior and fertility, sex reversal, and gonadal histopathology. However, few studies in fish assess the impacts of estrogenic exposure on a physiological endpoint, such as reproduction, as well as the associated morphologic response and underlying global gene expression changes. This study assessed the implications of a 14 day sub-chronic exposure of ethinylestradiol (EE2; 1.0 or 10.0 µg/L EE2) on male medaka fertility, testicular histology and testicular gene expression. The findings demonstrate that a 14 day exposure to EE2 induced impaired male reproductive capacity and time- and dose-dependent alterations in testicular morphology and gene expression. The average fertilization rate/day following the exposure for control, 1.0 and 10.0 µg/L EE2 was 91.3% (±4.4), 62.8% (±8.3) and 28.8% (±5.8), respectively. The testicular morphologic alterations included increased germ cell apoptosis, decreased germinal epithelium and thickening of the interstitium. These changes were highly associated with testicular gene expression changes using a medaka-specific microarray. A pathway analysis of the differentially expressed genes emphasized genes and pathways associated with apoptosis, cell cycle and proliferation, collagen production/extracellular matrix organization, hormone signaling, male reproduction and protein ubiquitination among others. These findings highlight the importance of anchoring global gonadal gene expression changes with morphology and ultimately with tissue/organ function.
authors.affiliation: Integrated Toxicology and Environmental Health Program, Nicholas School of the Environment, Duke University, Durham, North Carolina, United States of America.
authors.forename: Hilary D
authors.initials: HD
authors.lastname: Miller
authors.forename: Bryan W
authors.initials: BW
authors.lastname: Clark
authors.forename: David E
authors.initials: DE
authors.lastname: Hinton
authors.forename: Andrew
authors.initials: A
authors.lastname: Whitehead
authors.forename: Stan
authors.initials: S
authors.lastname: Martin
authors.forename: Kevin W
authors.initials: KW
authors.lastname: Kwok
authors.forename: Seth W
authors.initials: SW
authors.lastname: Kullman
mesh_terms: D000818:Animals; D001947:Breeding; D004967:Estrogens / Q000633:toxicity; D004997:Ethinyl Estradiol / Q000633:toxicity*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D009990:Oryzias / Q000033:anatomy & histology* / Q000235:genetics / Q000502:physiology*; D012098:Reproduction / Q000187:drug effects* / Q000235:genetics; D013045:Species Specificity; D013737:Testis / Q000033:anatomy & histology* / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D013997:Time Factors; D059467:Transcriptome / Q000187:drug effects*
chemical_list: D004967:Estrogens; D004997:Ethinyl Estradiol