title: Micropattern-controlled local microtubule nucleation, transport, and mesoscale organization.
abstract: Microtubule organization in living cells is determined by spatial control of microtubule nucleation, their dynamic properties, and transport by molecular motors. Here, we establish a new micropattern-guided method for controlling local microtubule nucleation by spatially confined immobilization of a microtubule polymerase and show that these nucleated microtubules can be transported and organized in space by motor proteins. This assay provides a new platform for deciphering the principles underlying mesoscale microtubule organization.
authors.forename: Surajit
authors.initials: S
authors.lastname: Ghosh
authors.forename: Christian
authors.initials: C
authors.lastname: Hentrich
authors.forename: Thomas
authors.initials: T
authors.lastname: Surrey
mesh_terms: D001692:Biological Transport; D008870:Microtubules / Q000201:enzymology / Q000378:metabolism*