title: [Effects of cromolyn sodium on intestinal ischemia-reperfusion injury by inhibiting PAR-2 expression in rats].
abstract: OBJECTIVE
  To explore the effects of cromolyn sodium (CS) on intestinal ischemia-reperfusion (IIR) and its relationship with mast cell activation and protease-activated receptor 2 (PAR-2) expression.
  
  
  METHODS
  A total of 32 SD rats were randomly divided into 4 groups: sham-operated (S), intestinal ischemia reperfusion (IIR), CS (a mast cell stabilizer, CS, 25 mg/kg) and compound 48/80 (a mast cell degranulation, CP, 0.75 mg/kg) (n = 8 each). IIR was induced by clamping superior mesenteric artery for 75 min followed by reperfusion for 3 hours. The above agents were intravenously administrated at 5 min pre-reperfusion. Rats were then sacrificed and intestinal issues harvested for histological examinations. The tryptase expression and mast cell count were analyzed by immunohistochemistry. PAR-2 was analyzed by Western blot.
  
  
  RESULTS
  The Chiu's score (0.75 ± 0.21), mast cell count (10 ± 3), tryptase expression (125 ± 15) and PAR-2 expression (109 ± 10) of group S were the least while those of group CP the most (all P < 0.05). The Chiu's score (2.14 ± 0.64), mast cell count (15 ± 4), tryptase expression (138 ± 17) and PAR-2 expression (124 ± 12) of group CS were less than those of groups IIR and CP (all P < 0.05).
  
  
  CONCLUSIONS
  Cromolyn sodium may reduce IIR injury by stabilizing mast cell membrane and inhibiting the expressions of tryptase and PAR-2.
authors.affiliation: Department of Anesthesiology, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: De-zhao
authors.initials: DZ
authors.lastname: Liu
authors.forename: Zhong-gang
authors.initials: ZG
authors.lastname: Chen
authors.forename: Mian
authors.initials: M
authors.lastname: Ge
authors.forename: Xiao-liang
authors.initials: XL
authors.lastname: Gan
authors.forename: Zi-qing
authors.initials: ZQ
authors.lastname: Hei
mesh_terms: D000818:Animals; D004205:Cromolyn Sodium / Q000494:pharmacology*; D005260:Female; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000473:pathology; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D044464:Receptor, PAR-2 / Q000378:metabolism*; D015427:Reperfusion Injury / Q000378:metabolism*
chemical_list: D044464:Receptor, PAR-2; D004205:Cromolyn Sodium