title: Complete genome sequence analysis of an American isolate of Grapevine virus E.
abstract: The complete genome sequence of Grapevine virus E (GVE) collected from a red-berried wine grape cultivar (Cabernet Sauvignon) in Washington State was determined. The 7,568 nucleotide long genome of GVE is more similar in sequence identity with a GVE isolate from a wine grape cv. Shiraz from South Africa when compared with an isolate from "Aki Queen" grape from Japan. Like GVE isolates from South Africa and Japan, the Washington isolate encodes five open reading frames (ORFs) and the overall genome organization is identical among these isolates. In addition to AlkB domain, a DExD domain, belonging to the DEAD-like helicases superfamily, was present upstream of the helicase domain in the replicase ORF of the virus.
authors.affiliation: Department of Plant Pathology, Irrigated Agriculture Research and Extension Center, Washington State University, Prosser, WA 99350, USA.
authors.forename: Olufemi J
authors.initials: OJ
authors.lastname: Alabi
authors.forename: Sudarsana
authors.initials: S
authors.lastname: Poojari
authors.forename: Kara
authors.initials: K
authors.lastname: Sarver
authors.forename: Robert R
authors.initials: RR
authors.lastname: Martin
authors.forename: Rayapati A
authors.initials: RA
authors.lastname: Naidu
mesh_terms: D016000:Cluster Analysis; D052677:Flexiviridae / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D027843:Vitis / Q000821:virology*; D014861:Washington
chemical_list: D012367:RNA, Viral