title: [Diversity and bioactivities of culturable marine actinobacteria isolated from mangrove sediment in Indian Ocean].
abstract: OBJECTIVE
  In order to explore the diversity, antimicrobial activity and enzyme-producing activity of marine actinobacteria isolated from mangrove sediments in Indian Ocean.
  
  
  METHODS
  Eight sediments collected from mangrove sediments in Indian Ocean were treated by the plate dilution method and spread on 24 isolation media only containing sole carbon source for energy. Marine actinobacteria were isolated and identified by 16S rRNA gene sequence analysis. The antimicrobial activity and enzyme-producing activity of isolated strains were further detected by spot planting method.
  
  
  RESULTS
  In total 139 representative strains were selected from 521 isolates, and they were further sequenced and performed phylogenetic analysis based on their 16S rRNA gene sequences. There were 35 strains identified as potential novel species. Antimicrobial activity was detected in Bacillus subtilis, Candida albicans, Escherichia coli, Staphylococcus aureus, Aspergillus niger. Enzyme-producing activity for protease cellulase, amylase and esterase were 36.5%, 26.5%, 22.4% and 15.9%, respectively.
  
  
  CONCLUSIONS
  Diverse marine actinobacteria were discovered in mangrove sediment in Indian Ocean, which have antimicrobial and enzyme activity.
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China. hejielovely@163.com
authors.forename: Jie
authors.initials: J
authors.lastname: He
authors.forename: Daofeng
authors.initials: D
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Zhang
authors.forename: Shukun
authors.initials: S
authors.lastname: Tang
authors.forename: Lihua
authors.initials: L
authors.lastname: Xu
authors.forename: Wenjun
authors.initials: W
authors.lastname: Li
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D044822:Biodiversity; D019015:Geologic Sediments / Q000382:microbiology*; D019156:Indian Ocean; D008969:Molecular Sequence Data; D010802:Phylogeny; D012623:Seawater / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins