title: Injury-elicited stressors alter endogenous retrovirus expression in lymphocytes depending on cell type and source lymphoid organ.
abstract: BACKGROUND
  Murine leukemia virus-type endogenous retroviruses (MuLV-ERVs) constitute ~10% of the mouse genome and are associated with various pathophysiologic processes. In this study, we examined whether MuLV-ERVs' response to burn-elicited stressors is specific for certain lymphocyte populations and/or locations of lymphoid organ.
  
  
  RESULTS
  B- and T-cells, which were sorted from nine lymphoid organs of C57BL/6J mice after burn, were subjected to MuLV-ERV expression analyses. Overall, the post-burn MuLV-ERV expression pattern was dependent on lymphocyte type, time after injury, location of lymphoid organ, and MuLV-ERV type. For instance, the MuLV-ERV expression in T-cells from the thymus and three cervical lymph nodes decreased at 3 hours post-burn while the expression of some MuLV-ERVs was augmented in B-cells derived from the mesenteric lymph node. The MuLV-ERV U3 sequences population of the burn-24 hours group was less diverse in comparison to the no burn and burn-3 hours groups. In addition, it was apparent that at the 24 hours time point, the U3 populations of B-cells from both no burn and burn groups were less heterogeneous than the T-cells' U3 populations. Using the U3 sequences, some of which were isolated only from specific experimental groups (B- vs. T-cells; no burn vs. burn), as probes, 51 putative MuLV-ERVs, including 16 full-length proviruses, were mapped followed by characterization of their biologic properties.
  
  
  CONCLUSIONS
  MuLV-ERVs' response to burn-elicited stressors may be differentially controlled depending on lymphocyte type, location of lymphoid organ, MuLV-ERV type, and stress duration.
authors.affiliation: Department of Surgery, Burn Research, Shriners Hospitals for Children Northern California, University of California, Davis, Sacramento, CA 95817, USA.
authors.forename: Kang-Hoon
authors.initials: KH
authors.lastname: Lee
authors.forename: Debora
authors.initials: D
authors.lastname: Lim
authors.forename: Tajia
authors.initials: T
authors.lastname: Green
authors.forename: David
authors.initials: D
authors.lastname: Greenhalgh
authors.forename: Kiho
authors.initials: K
authors.lastname: Cho
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000821:virology*; D001483:Base Sequence; D002056:Burns / Q000276:immunology* / Q000821:virology*; D002469:Cell Separation; D003001:Cloning, Molecular; D019295:Computational Biology; D020077:Endogenous Retroviruses / Q000235:genetics*; D005260:Female; D015967:Gene Expression Regulation, Viral; D056426:Genetic Loci / Q000235:genetics; D016678:Genome / Q000235:genetics; D009052:Leukemia Virus, Murine / Q000235:genetics*; D008221:Lymphoid Tissue / Q000276:immunology / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010802:Phylogeny; D011401:Promoter Regions, Genetic / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology / Q000821:virology*; D014158:Transcription, Genetic