title: Derivation of human induced pluripotent stem cells from patients with maturity onset diabetes of the young.
abstract: Maturity onset diabetes of the young (MODY) is an autosomal dominant disease. Despite extensive research, the mechanism by which a mutant MODY gene results in monogenic diabetes is not yet clear due to the inaccessibility of patient samples. Induced pluripotency and directed differentiation toward the pancreatic lineage are now viable and attractive methods to uncover the molecular mechanisms underlying MODY. Here we report, for the first time, the derivation of human induced pluripotent stem cells (hiPSCs) from patients with five types of MODY: MODY1 (HNF4A), MODY2 (GCK), MODY3 (HNF1A), MODY5 (HNF1B), and MODY8 (CEL) with a polycistronic lentiviral vector expressing a Cre-excisable human "stem cell cassette" containing the four reprogramming factors OCT4, KLF4, SOX2, and CMYC. These MODY-hiPSCs morphologically resemble human pluripotent stem cells (hPSCs), express pluripotency markers OCT4, SOX2, NANOG, SSEA-4, and TRA-1-60, give rise to derivatives of the three germ layers in a teratoma assay, and are karyotypically normal. Overall, our MODY-hiPSCs serve as invaluable tools to dissect the role of MODY genes in the development of pancreas and islet cells and to evaluate their significance in regulating beta cell function. This knowledge will aid future attempts aimed at deriving functional mature beta cells from hPSCs.
authors.affiliation: Section of Islet Cell and Regenerative Biology, Joslin Diabetes Center, Department of Medicine, Brigham and Women's Hospital, and Harvard Medical School, Boston, Massachusetts 02215, USA.
authors.forename: Adrian K K
authors.initials: AK
authors.lastname: Teo
authors.forename: Rebecca
authors.initials: R
authors.lastname: Windmueller
authors.forename: Bente B
authors.initials: BB
authors.lastname: Johansson
authors.forename: Ercument
authors.initials: E
authors.lastname: Dirice
authors.forename: Pal R
authors.initials: PR
authors.lastname: Njolstad
authors.forename: Erling
authors.initials: E
authors.lastname: Tjora
authors.forename: Helge
authors.initials: H
authors.lastname: Raeder
authors.forename: Rohit N
authors.initials: RN
authors.lastname: Kulkarni
mesh_terms: D000293:Adolescent; D000328:Adult; D001706:Biopsy / Q000379:methods; D018929:Cell Culture Techniques / Q000379:methods*; D002648:Child; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D005786:Gene Expression Regulation; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D050417:Insulin-Secreting Cells / Q000166:cytology; D007515:Islets of Langerhans / Q000166:cytology; D007621:Karyotyping; D000090062:Kruppel-Like Factor 4; D008297:Male; D008875:Middle Aged; D008957:Models, Genetic; D010179:Pancreas / Q000378:metabolism; D010375:Pedigree; D012867:Skin / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D014157:Transcription Factors