title: The effect of Bcl-2 siRNA combined with miR-15a oligonucleotides on the growth of Raji cells.
abstract: Bcl-2 is a prominent member of the Bcl-2 family of proteins that regulate the induction of apoptosis. Here, we investigated the effect of Bcl-2 small interfering RNA (siRNA) combined with miR-15a oligonucleotides (ODN) on the growth of Raji cells. The expression levels of Bcl-2 mRNA and protein were detected. The inhibition of cell growth and apoptosis was determined. Atomic force microscopy was utilized to observe the cell-surface ultrastructural changes. After transfection of combination, protein and mRNA levels of Bcl-2 obviously were decreased. The growth of cells was significantly inhibited compared with those cells transfected with Bcl-2 siRNA or miR-15a alone. Apoptotic rate significantly increased. The cells of apoptosis showed a roughness variation on cell membranes. These results suggest that the combination of Bcl-2 siRNA and miR-15a ODN increases apoptosis in Raji cells. Our study implies that the combination of Bcl-2 siRNA and miR-15a may be a useful approach in treatment for lymphoma.
authors.affiliation: Institute of Hematology, Medical College, Jinan University, No. 601, West Huangpu Road, Guangzhou 510632, China.
authors.forename: Xiaomao
authors.initials: X
authors.lastname: Hu
authors.forename: Wenyu
authors.initials: W
authors.lastname: Li
authors.forename: Gexiu
authors.initials: G
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Wu
authors.forename: Yangjun
authors.initials: Y
authors.lastname: Gao
authors.forename: Shengting
authors.initials: S
authors.lastname: Chen
authors.forename: Dongmei
authors.initials: D
authors.lastname: He
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005786:Gene Expression Regulation / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D018625:Microscopy, Atomic Force; D009841:Oligonucleotides / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D035683:MicroRNAs; D009841:Oligonucleotides; D019253:Proto-Oncogene Proteins c-bcl-2; D034741:RNA, Small Interfering