title: Novel synthesis strategy for the preparation of individual phytosterol oxides.
abstract: Sterols (cholesterol and phytosterols) are important structural components of cell membranes and major constituents of lipid metabolism. Research on their oxides, such as the factors affecting oxidation, oxides' structures, and qualitative and quantitative analysis, aroused more attention in this decade. However, the biological roles of individual phytosterol oxides are still unclear because no commercial individual phytosterol oxide standards are available. Different from the traditional chemical synthesis, in the present study, chemical synthesis from a starting phytosterol mixture followed with a semipreparative HPLC separation produced individual oxides. TLC and analytical HPLC were used here to not only monitor the reaction process but also specifically analyze the synthetic intermediates and oxides. The chromatographic results exhibited strict rules and similar characteristics. Finally, for the first time, four individual phytosterol oxides were successfully separated and collected by a semipreparative HPLC system, thus providing a novel strategy for the preparation of individual phytosterol oxides.
authors.affiliation: School of Life Sciences, Anhui Agricultural University, Hefei 230036, People's Republic of China.
authors.forename: Junlan
authors.initials: J
authors.lastname: Gao
authors.forename: Qiulin
authors.initials: Q
authors.lastname: Yue
authors.forename: Yishun
authors.initials: Y
authors.lastname: Ji
authors.forename: Beijiu
authors.initials: B
authors.lastname: Cheng
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D002784:Cholesterol / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer; D010084:Oxidation-Reduction; D010087:Oxides / Q000138:chemical synthesis*; D010840:Phytosterols / Q000138:chemical synthesis* / Q000302:isolation & purification; D013265:Stigmasterol / Q000737:chemistry
chemical_list: D010087:Oxides; D010840:Phytosterols; D002784:Cholesterol; D013265:Stigmasterol