title: [HCV replication in Huh-7.5 cell line].
abstract: BACKGROUND
  Infection with hepatitis C virus is a serious worldwide health problem. Since its discovery in 1989, the development of a cell culture system for HCV has been a major goal for scientists worldwide. In 2005 the first tissue culture that led to the production of HCV particles (2a genotype) has been created. The aim of the study was to determine viral RNA level in an infectious HCV cell culture system.
  
  
  METHODS
  Huh-7.5 cell line was infected with the JFH1 (Japanese Fulminant Hepatitis) RNA by lipofection. The level of HCV RNA was measured in supernatant of the cell culture by Real-Time PCR method.
  
  
  RESULTS
  HCV RNA was detected in the supernatant of Huh-7.5 cell line infected with the use ofJFH 1 RNA and lipofectamin. The maximal level of HCV RNA (3.69 x 10(9) copies/ml) was detected 96h after transfection. After about 30 days oftransfection, HCV RNA was on the stable level (10(7)-10(8)).
  
  
  CONCLUSIONS
  Huh-7.5 cell line infected with HCV form a robust cell culture model of HCV infection. HCV replicates on high levels in Huh-7.5, which is the crucial event for the investigation of new antivirals in in vitro model.
authors.affiliation: Pracownia Immunopatologii Zakazen Hepatotropowych, Zakładu Wirusologii Narodowego Instytutu Zdrowia Publicznego--Państwowego Zakładu Higieny w Warszawie.
authors.forename: Marcin
authors.initials: M
authors.lastname: Komorowski
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kołakowska
authors.forename: Paulina
authors.initials: P
authors.lastname: Godzik
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Madaliński
mesh_terms: D002460:Cell Line; D016174:Hepacivirus / Q000502:physiology*; D006801:Humans; D012367:RNA, Viral / Q000302:isolation & purification*; D014779:Virus Replication / Q000235:genetics*
chemical_list: D012367:RNA, Viral