title: Resolvin D1 reduces the immunoinflammatory response of the rat eye following uveitis.
abstract: This study investigated whether the administration of resolvin D1 to rats with endotoxininduced uveitis (EIU) ameliorates the immuno-inflammatory profile of the eye. 24 h after the administration of 200 μg LPS into the footpad of Sprague-Dawley rats, severe changes of the structure of the eye occurred concomitantly with a severe inflammatory and immune response. These latter included strong infiltration of PMN leukocytes CD11b(+) T-lymphocytes CD4(+) and CD8(+) within the eye and a significant release of the cytokines/chemokines TNF-alpha, CXCL8, and RANTES too. Bolus of resolvin D1 (RvD1; 10-100-1000 ng/kg in 200 μL of sterile saline via the tail vein) significantly and dose-dependently (i) reduced the development of the ocular derangement caused by LPS; (ii) reduced the clinical score attributed to EIU; (iii) reduced the protein concentration and myeloperoxidase activity (MPO) in aqueous humor (AqH); and (iv) reduced neutrophils, T-lymphocytes, and cytokines within the eye.
authors.affiliation: Department of Ophthalmology, Second University of Naples, 80100 Naples, Italy.
authors.forename: Rossi
authors.initials: R
authors.lastname: Settimio
authors.forename: Di Filippo
authors.initials: DF
authors.lastname: Clara
authors.forename: Ferraraccio
authors.initials: F
authors.lastname: Franca
authors.forename: Simonelli
authors.initials: S
authors.lastname: Francesca
authors.forename: D'Amico
authors.initials: D
authors.lastname: Michele
mesh_terms: D000818:Animals; D018925:Chemokines / Q000032:analysis; D016207:Cytokines / Q000032:analysis; D004281:Docosahexaenoic Acids / Q000627:therapeutic use*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013601:T-Lymphocytes / Q000276:immunology; D014605:Uveitis / Q000188:drug therapy* / Q000276:immunology
chemical_list: D018925:Chemokines; D016207:Cytokines; C518399:resolvin D1; D004281:Docosahexaenoic Acids