title: Suppression of carbonic anhydrase IX leads to aberrant focal adhesion             and decreased invasion of tumor cells.
abstract: Carbonic anhydrase IX (CA IX) is a well-recognized hypoxia marker with promising             diagnostic and therapeutic value. CA IX regulates the pH in hypoxic tumor cells             and, thereby, contributes to microenvironmental acidosis and cell migration. To             gain a better insight into the molecular processes driven by CA IX, we performed             gene expression profiling of HT-1080 fibrosarcoma cells subjected to CA IX depletion             by shRNA silencing. We identified the focal adhesion pathway as being significantly             inhibited in the absence of CA IX and confirmed this finding by functional assays.             Thus, we obtained the first direct evidence for the role of CA IX in focal adhesion.
authors.affiliation: Institute of Virology, Department of Molecular Medicine, Slovak                     Academy of Sciences, 845 05 Bratislava, Slovak Republic.
authors.forename: Peter
authors.initials: P
authors.lastname: Radvak
authors.forename: Marko
authors.initials: M
authors.lastname: Repic
authors.forename: Eliska
authors.initials: E
authors.lastname: Svastova
authors.forename: Martina
authors.initials: M
authors.lastname: Takacova
authors.forename: Lucia
authors.initials: L
authors.lastname: Csaderova
authors.forename: Hynek
authors.initials: H
authors.lastname: Strnad
authors.forename: Jaromir
authors.initials: J
authors.lastname: Pastorek
authors.forename: Silvia
authors.initials: S
authors.lastname: Pastorekova
authors.forename: Juraj
authors.initials: J
authors.lastname: Kopacek
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics* / Q000378:metabolism; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases / Q000235:genetics* / Q000378:metabolism; D002448:Cell Adhesion; D002465:Cell Movement; D022001:Focal Adhesions / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D009361:Neoplasm Invasiveness; D034741:RNA, Small Interfering / Q000235:genetics; D059467:Transcriptome
chemical_list: D000951:Antigens, Neoplasm; D034741:RNA, Small Interfering; C089226:CA9 protein, human; D000071231:Carbonic Anhydrase IX; D002256:Carbonic Anhydrases