title: Identification of a novel Pfkfb1 mRNA variant in rat fetal liver.
abstract: The bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase-2) catalyzes the synthesis and degradation of fructose-2,6-bisphosphate, a key metabolite in the glucose homeostasis. Four genes, Pfkfb1-4, have been characterized in mammals that code for several isoforms generated by alternative splicing through the control of several promoters and 5' non-coding exons. Here, we characterize in fetal rat liver new mRNA variants which are transcribed from a new Pfkfb1 gene promoter. The long variant codes to a new isoform (FL-PFK-2) that would be of relevant function to modulate the transition of fetal to adult liver metabolism.
authors.affiliation: Instituto de Biomedicina de Valencia, IBV-CSIC, Jaime Roig 11, 46010 Valencia, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Cosin-Roger
authors.forename: Santiago
authors.initials: S
authors.lastname: Vernia
authors.forename: Maria Soledad
authors.initials: MS
authors.lastname: Alvarez
authors.forename: Carme
authors.initials: C
authors.lastname: Cucarella
authors.forename: Lisardo
authors.initials: L
authors.lastname: Boscá
authors.forename: Paloma
authors.initials: P
authors.lastname: Martin-Sanz
authors.forename: Ana Julia
authors.initials: AJ
authors.lastname: Fernández-Alvarez
authors.forename: Marta
authors.initials: M
authors.lastname: Casado
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D005333:Fetus / Q000201:enzymology*; D057809:HEK293 Cells; D006801:Humans; D008099:Liver / Q000196:embryology / Q000201:enzymology*; D025481:Phosphofructokinase-2 / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats
chemical_list: D012333:RNA, Messenger; D025481:Phosphofructokinase-2