title: The creation of cybrids harboring mitochondrial haplogroups in the Taiwanese population of ethnic Chinese background: an extensive in vitro tool for the study of mitochondrial genomic variations.
abstract: Mitochondrial DNA (mtDNA) haplogroups may contribute to the development of aging-related diseases. A reliable in vitro cellular system for investigating the physiologic significance of mtDNA haplogroups is essential. This study aims to construct and characterize a series of cybrid cell lines harboring variant mtDNA haplogroups collected from healthy Taiwanese volunteers. Cybrid cells harboring different mtDNA haplogroups like B4a, B4b, B4c, B4d, B5, R, F1a, F2, D4e, D4a, D5b, D5a, E, M8, C, and N9a were prepared. Luminex 1000 and full-length mtDNA sequencing were used to confirm that mtDNA haplogroups of transmitochondrial cybrids were identical to their original donors. Cybrid B4b had a significantly lower oxygen consumption rate and higher mitochondrial membrane potential compared to F1a, B5, D5a, D4a, and N9a but had more susceptibility to H(2)O(2)-induced oxidative stress than cybrid F1a, D4a, and N9a. Cybrid N9a had better oxygen consumption and H(2)O(2)-challenged viability compared to B4b, F1a, B5, D5a, and D4a. A series of cybrid cells harboring the main haplogroups of the Taiwanese population with ethnic Chinese background has been developed in vitro. With this mtDNA haplogroup population, the underlying mechanisms of aging-related diseases may be better understood, and therapeutic interventions can be accelerated.
authors.affiliation: Department of Neurology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, Taiwan.
authors.forename: Tsu-Kung
authors.initials: TK
authors.lastname: Lin
authors.forename: Hung-Yu
authors.initials: HY
authors.lastname: Lin
authors.forename: Shang-Der
authors.initials: SD
authors.lastname: Chen
authors.forename: Yao-Chung
authors.initials: YC
authors.lastname: Chuang
authors.forename: Jiin-Haur
authors.initials: JH
authors.lastname: Chuang
authors.forename: Pei-Wen
authors.initials: PW
authors.lastname: Wang
authors.forename: Sheng-Teng
authors.initials: ST
authors.lastname: Huang
authors.forename: Mao-Meng
authors.initials: MM
authors.lastname: Tiao
authors.forename: Jin-Bor
authors.initials: JB
authors.lastname: Chen
authors.forename: Chia-Wei
authors.initials: CW
authors.lastname: Liou
mesh_terms: D044466:Asian People / Q000235:genetics*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D002459:Cell Fusion; D045744:Cell Line, Tumor; D019069:Cell Respiration / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004272:DNA, Mitochondrial / Q000235:genetics*; D045222:Electron Transport Chain Complex Proteins / Q000378:metabolism; D004996:Ethidium / Q000378:metabolism; D005006:Ethnicity / Q000235:genetics*; D018628:Gene Dosage; D005819:Genetic Markers; D014644:Genetic Variation; D054629:Genome, Mitochondrial / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D011110:Polymorphism, Genetic; D013624:Taiwan; D014019:Tissue Donors
chemical_list: D004272:DNA, Mitochondrial; D045222:Electron Transport Chain Complex Proteins; D005819:Genetic Markers; D006861:Hydrogen Peroxide; D004996:Ethidium