title: Role of estrogen receptor in astringinin-mediated attenuation of intestinal injury after trauma-hemorrhage.
abstract: Astringinin protects against organ injury caused by trauma-hemorrhage though the mechanism remains unknown.  We hypothesize that astringinin administration in males following trauma-hemorrhage protects against intestinal injury through an estrogen receptor-dependent pathway.  To test this hypothesis, male Sprague-Dawley rats were subject to trauma-hemorrhage (mean blood pressure: 40 mmHg for 90 min) followed by fluid resuscitation.  Animals were pretreated with an estrogen receptor antagonist (ICI 182,780) 30 min before vehicle or astringinin (0.3 mg/kg) administration, followed by resuscitation, and the treated rats were killed 24 h thereafter.  Intestinal wet/dry weight ratio, myeloperoxidase (MPO) activity, and the levels of interleukin (IL)-6, intercellular adhesion molecule (ICAM)-1, cytokine-induced neutrophil chemoattractant (CINC)-1 and CINC-3 were measured.  Traumahemorrhage led to an increase in intestinal wet/dry weight ratio, MPO activity, histological damage and also increases in the levels of IL-6, ICAM-1, CINC-1 and CINC-3.  Administration of astringinin improved all of the above parameters.  Administration of ICI 182,780 with astringinin abolished the astringinin-mediated improvement of the above parameters.  These results suggest that the estrogen receptor-dependent pathway likely plays a critical role in mediating the salutary effects of astringinin on shock-induced intestinal injury.
authors.affiliation: Department of Anesthesiology, Chang Gung Memorial Hospital College of Medicine, Taoyuan 33305, Taiwan, Republic of China.
authors.forename: Huang-Ping
authors.initials: HP
authors.lastname: Yu
authors.forename: Fu-Chao
authors.initials: FC
authors.lastname: Liu
mesh_terms: D000818:Animals; D054360:Chemokine CXCL1 / Q000378:metabolism; D006470:Hemorrhage; D015850:Interleukin-6 / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen
chemical_list: D054360:Chemokine CXCL1; D015850:Interleukin-6; D011960:Receptors, Estrogen; D009195:Peroxidase