title: Analytical contribution of NAD 2D-NMR spectroscopy in polypeptide mesophases to the investigation of triglycerides.
abstract: In this work, we report and discuss on the use and limitations of the natural abundance deuterium two-dimensional NMR spectroscopy in polypeptide chiral and achiral aligning media in the studies of homogenous triglycerides at 14.1 T. As illustrative examples, two triglycerides with short and long alkyl chains were investigated: the 1,3-di(butanoyloxy)propan-2-yl butanoate or tributyrin (TB) and the 1,3-di(tetradecanoyloxy)propan-2-yl tetradecanoate or trimyristin (TM). If both flexible compounds are theoretically of C(s) symmetry on average, according to the Altmann's definition (Proc. Roy. Soc., 1967, A298, 184.), the analysis of spectral data in terms of enantiotopic and diastereotopic discriminations shows noticeable differences related to their orientational ordering behavior inside the mesophases. Although from NMR analysis viewpoint, TB behaves as a C(s) symmetry molecule as expected, the NMR results obtained for TM suggest a behavior that could be formally predicted for a C(3v) symmetry molecule on average. This conclusion was nicely supported by the comparison with the tri-n-propylorthoformate, a real C(3v) symmetry solute on average on the NMR timescale. This difference of effective orientational behavior could originate from the difference of size and shape between lateral and central alkyl chains of the solute molecule.
authors.affiliation: RMN en Milieu Orienté, ICMMO, UMR CNRS 8182, Université de Paris-Sud, 15 rue Georges Clémenceau, 91405 Orsay cedex, France. philippe.lesot@u-psud.fr
authors.forename: Philippe
authors.initials: P
authors.lastname: Lesot
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Serhan
authors.forename: Christie
authors.initials: C
authors.lastname: Aroulanda
authors.forename: Isabelle
authors.initials: I
authors.lastname: Billault
mesh_terms: D003903:Deuterium / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010455:Peptides / Q000032:analysis*; D014280:Triglycerides / Q000032:analysis*
chemical_list: D010455:Peptides; D014280:Triglycerides; D003903:Deuterium