title: Tumor-suppressive effects of 1, 25-dihydroxyvitamin D3 in gastric cancer cells.
abstract: OBJECTIVE
  It has been previously demonstrated that vitamin D acts as a prognostic indicator of gastric cancer and may be correlated with the incidence risk of gastric cancer. However, the effect of 1,25-dihydroxyvitamin D3 on the apoptosis of human gastric cancer cells is unclear. The aim of this study was to determine whether 1,25-dihydroxyvitamin D3 induced the cellular apoptosis of BGC-823 gastric cancer cells and to determine the potential mechanism of action.
  
  
  METHODS
  We demonstrate that 1,25-dihydroxyvitamin D3 induced the apoptosis of gastric cancer cells via the processing of PARP and cleavage of caspase 3. Additionally, an increase in BAX expression and a decrease in ERK1/2 and AKT phosphorylation were associated with 1,25-dihydroxyvitamin D3-induced apoptosis. The mRNA expression levels of VDR, CYP24A1, and p21 were increased significantly following 1,25-dihydroxyvitamin D3 treatment.
  
  
  CONCLUSIONS
  These findings suggest that 1,25-dihydroxyvitamin D3 exerts tumor-suppressive effects on BGC-823 human gastric cancer cells.
authors.forename: Anyu
authors.initials: A
authors.lastname: Bao
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Tong
authors.forename: Hongyun
authors.initials: H
authors.lastname: Zheng
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Chuandong
authors.initials: C
authors.lastname: Wei
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D020935:MAP Kinase Signaling System / Q000187:drug effects; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000187:drug effects; D018167:Receptors, Calcitriol / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013250:Steroid Hydroxylases / Q000187:drug effects; D013274:Stomach Neoplasms / Q000188:drug therapy*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000494:pharmacology; D065668:Vitamin D3 24-Hydroxylase; D051028:bcl-2-Associated X Protein / Q000187:drug effects
chemical_list: D050759:Cyclin-Dependent Kinase Inhibitor p21; D018167:Receptors, Calcitriol; C579457:VDR protein, human; D051028:bcl-2-Associated X Protein; C118756:dihydroxy-vitamin D3; D014807:Vitamin D; D013250:Steroid Hydroxylases; C585549:CYP24A1 protein, human; D065668:Vitamin D3 24-Hydroxylase; D051057:Proto-Oncogene Proteins c-akt