title: Chryseobacterium frigidisoli sp. nov., a psychrotolerant species of the family Flavobacteriaceae isolated from sandy permafrost from a glacier forefield.
abstract: During diversity studies of the glacier forefields of the Larsemann Hills, East Antarctica, a novel psychrotolerant, non-motile Gram-negative, shiny yellow, rod-shaped, aerobic bacterium, designated strain PB4(T) was isolated from a soil sample. Strain PB4(T) produces indole from tryptophan and hydrolyses casein. It grows between 0 and 25 °C with an optimum growth temperature of 20 °C. A wide range of substrates are used as sole carbon sources and acid is produced from numerous carbohydrates. The major menaquinone is MK-6. Identified polar lipids are ethanolamines and ornithine lipids. Major fatty acids (>10 %) are iso-C15 : 0 (13.0 %) and iso-2OH-C15 : 0 (51.2 %). G+C content is 33.7 mol%. The polyamine pattern is composed of sym-homospermidine (25.1 µmol g(-1) dry weight), minor amounts of cadaverine (0.2 µmol g(-1) dry weight) and spermidine (0.4 µmol g(-1) dry weight) and traces of putrescine and spermine (<0.1 µmol g(-1) dry weight). Strain PB4(T) had highest 16S rRNA gene similarities with the type strains of Chryseobacterium humi (97.0 %) and Chryseobacterium marinum (96.5 %). Considering phenotypic and genotypic characterization, strain PB4(T) represents a novel species in the genus Chryseobacterium (family Flavobacteriaceae), for which the name Chryseobacterium frigidisoli sp. nov. is proposed. The type strain is PB4(T) ( = DSM 26000(T) = LMG 27025(T)).
authors.affiliation: Alfred Wegener Institute for Polar and Marine Research, Research Department Potsdam, Germany.
authors.forename: Felizitas
authors.initials: F
authors.lastname: Bajerski
authors.affiliation: University of Tromsø, Department for Arctic and Marine Biology, Tromsø, Norway.
authors.forename: Lars
authors.initials: L
authors.lastname: Ganzert
authors.affiliation: Helmholtz Centre Potsdam, GFZ German Research Centre for Geosciences, Section 4.3, Organic Geochemistry, Germany.
authors.forename: Kai
authors.initials: K
authors.lastname: Mangelsdorf
authors.affiliation: Alfred Wegener Institute for Polar and Marine Research, Research Department Potsdam, Germany.
authors.forename: Lisa
authors.initials: L
authors.lastname: Padur
authors.affiliation: Rheinische Friedrich-Wilhelms-Universität Bonn, Institute of Nutrition and Food Sciences, Department of Food Microbiology and Hygiene, Bonn, Germany.
authors.forename: André
authors.initials: A
authors.lastname: Lipski
authors.affiliation: Alfred Wegener Institute for Polar and Marine Research, Research Department Potsdam, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Wagner
mesh_terms: D000864:Antarctic Regions; D015373:Bacterial Typing Techniques; D045247:Chryseobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D046448:Ice Cover / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011073:Polyamines / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011073:Polyamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6