title: Enhancement of invertase production by Aspergillus niger OZ-3 using low-intensity static magnetic fields.
abstract: The aim of this study is to investigate the effect of low-intensity static magnetic fields (SMFs) on invertase activity and growth on different newly identified molds. The most positive effect of SMFs on invertase activity and growth was observed for Aspergillus niger OZ-3. The submerged production of invertase was performed with the spores obtained at the different exposure times (120, 144, 168, and 196 hr) and magnetic field intensities (0.45, 3, 5, 7, and 9 mT). The normal magnetic field of the laboratory was assayed as 0.45 mT (control). Optimization of magnetic field intensity and exposure time significantly increased biomass production and invertase activity compared to 0.45 mT. The maximum invertase activity (51.14 U/mL) and biomass concentration (4.36 g/L) were achieved with the spores obtained at the 144 hr exposure time and 5 mT magnetic field intensity. The effect of low-intensity static magnetic fields (SMFs) on invertase activities of molds was investigated for the first time in the present study. As an additional contribution, a new hyper-invertase-producing mold strain was isolated.
authors.affiliation: Department of Molecular Biology and Genetics, Science Faculty, Ataturk University, Erzurum, Turkey. mesuttaskin61@hotmail.com
authors.forename: Mesut
authors.initials: M
authors.lastname: Taskin
authors.forename: Nevzat
authors.initials: N
authors.lastname: Esim
authors.forename: Mucip
authors.initials: M
authors.lastname: Genisel
authors.forename: Serkan
authors.initials: S
authors.lastname: Ortucu
authors.forename: Ismet
authors.initials: I
authors.lastname: Hasenekoglu
authors.forename: Ozden
authors.initials: O
authors.lastname: Canli
authors.forename: Serkan
authors.initials: S
authors.lastname: Erdal
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology* / Q000254:growth & development; D004789:Enzyme Activation; D057075:Enzyme Assays; D005656:Fungal Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D060526:Magnetic Fields; D008280:Magnetics / Q000379:methods; D012988:Soil Microbiology; D013170:Spores / Q000201:enzymology; D055672:Static Electricity; D013395:Sucrose / Q000378:metabolism; D013997:Time Factors; D043324:beta-Fructofuranosidase / Q000096:biosynthesis* / Q000302:isolation & purification
chemical_list: D005656:Fungal Proteins; D013395:Sucrose; D043324:beta-Fructofuranosidase