title: Cationic cholesterol-modified gelatin as an in vitro siRNA delivery vehicle.
abstract: Polymeric micelle prepared through the self-assembly of cationic cholesterol-modified gelatin was tested for siRNA delivery. It exerted the desired effect of gene knockdown in HeLa cells stably expressing the luciferase gene and achieved a two-fold increase in the knockdown ability when compared to Lipofectamine® 2000. It was found that the polymeric micelle exhibited excellent stability and increased the biological stability of the siRNA in serum.
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wakoshi, Saitama 351-0198, Japan.
authors.forename: Pallavi Ananda
authors.initials: PA
authors.lastname: Kadengodlu
authors.forename: Toshiro
authors.initials: T
authors.lastname: Aigaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Abe
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
mesh_terms: D052160:Benzothiazoles; D002412:Cations / Q000737:chemistry; D002470:Cell Survival; D002784:Cholesterol / Q000737:chemistry*; D003959:Diamines; D005780:Gelatin / Q000737:chemistry* / Q000378:metabolism; D055785:Gene Knockdown Techniques / Q000379:methods; D018014:Gene Transfer Techniques; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008823:Micelles; D008856:Microscopy, Fluorescence; D008956:Models, Chemical; D008958:Models, Molecular; D009930:Organic Chemicals / Q000737:chemistry; D011485:Protein Binding; D011804:Quinolines; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015203:Reproducibility of Results
chemical_list: D052160:Benzothiazoles; D002412:Cations; D003959:Diamines; D008823:Micelles; D009930:Organic Chemicals; D011804:Quinolines; D034741:RNA, Small Interfering; C098022:SYBR Green I; D005780:Gelatin; D002784:Cholesterol; D008156:Luciferases