title: A serum-tolerant hydroxyl-modified polyethylenimine as versatile carriers of pDNA/siRNA.
abstract: The polyethylenimine (PEI) derivatives (PTn) are prepared by treating PEI25k with Tris(hydroxymethyl) acrylamidomethane via the Michael addition. These PTns can effectively condense nucleic acids into nanosized particles with positive surface charges. The PTns show lower cytotoxicity and better serum-resistant capacity than PEI25k. Specially, the transfection efficiency of PT26/DNA is 29-fold higher than that of PEI25k in HeLa cells in serum-containing medium. The PTn/siRNA complexes show superior knockdown effect in CT26 cells in serum-containing medium. In addition, flow cytometry analysis shows that the PTns can efficiently mediate the entry of nucleic acids into the cell. Thus, PTns are potentially applicable as non-viral carriers of nucleic acids and warrant further development for use in gene therapy.
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China; Graduate School of Chinese Academy of Sciences, Beijing 100039, P. R. China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Dong
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Zhaopei
authors.initials: Z
authors.lastname: Guo
authors.forename: Huayu
authors.initials: H
authors.lastname: Tian
authors.forename: Yuce
authors.initials: Y
authors.lastname: Li
authors.forename: Yen
authors.initials: Y
authors.lastname: Wei
authors.forename: Xuesi
authors.initials: X
authors.lastname: Chen
mesh_terms: D000588:Amines / Q000032:analysis; D016503:Drug Delivery Systems / Q000379:methods*; D024202:Electrophoretic Mobility Shift Assay; D005434:Flow Cytometry; D020868:Gene Silencing; D015316:Genetic Therapy / Q000379:methods*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D017665:Hydroxyl Radical / Q000737:chemistry; D008156:Luciferases; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011094:Polyethyleneimine / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000378:metabolism*; D014162:Transfection / Q000379:methods
chemical_list: D000588:Amines; D034741:RNA, Small Interfering; D017665:Hydroxyl Radical; D011094:Polyethyleneimine; D008156:Luciferases