title: A cyclic peptide mimic of an RNA recognition motif of human La protein is a potent inhibitor of hepatitis C virus.
abstract: Due to limited available therapeutic options, developing new lead compounds against hepatitis C virus is an urgent need. Human La protein stimulates hepatitis C virus translation through interaction with the hepatitis C viral RNA. A cyclic peptide mimicking the β-turn of the human La protein that interacts with the viral RNA was synthesized. It inhibits hepatitis C viral RNA translation significantly better than the corresponding linear peptide at longer post-treatment times. The cyclic peptide also inhibited replication as measured by replicon RNA levels using real time RT-PCR. The cyclic peptide emerges as a promising lead compound against hepatitis C.
authors.affiliation: Division of Structural Biology and Bioinformatics, CSIR-Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Kolkata 700 032, India.
authors.forename: Asit Kumar
authors.initials: AK
authors.lastname: Manna
authors.forename: Anuj
authors.initials: A
authors.lastname: Kumar
authors.forename: Upasana
authors.initials: U
authors.lastname: Ray
authors.forename: Saumitra
authors.initials: S
authors.lastname: Das
authors.forename: Gautam
authors.initials: G
authors.lastname: Basu
authors.forename: Siddhartha
authors.initials: S
authors.lastname: Roy
mesh_terms: D020816:Amino Acid Motifs; D000998:Antiviral Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D016174:Hepacivirus / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D006526:Hepatitis C / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D006801:Humans; D010456:Peptides, Cyclic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D010750:Phosphoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D014176:Protein Biosynthesis / Q000187:drug effects; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; C574674:La protein, human; D010456:Peptides, Cyclic; D010750:Phosphoproteins; D014764:Viral Proteins