title: Metal-organic framework MIL-100(Fe) as the stationary phase for both normal-phase and reverse-phase high performance liquid chromatography.
abstract: Metal-organic framework MIL-100(Fe) was explored as a novel stationary phase for both normal-phase and reverse-phase high performance liquid chromatography. Two groups of analytes (benzene, toluene, ethylbenzene, naphthalene and 1-chloronaphthalene; aniline, acetanilide, 2-nitroaniline and 1-naphthylamine) were used to test the separation performance of MIL-100(Fe) in the reverse-phase mode, while the isomers of chloroaniline or toluidine were employed to evaluate its performance in the normal-phase mode. The MIL-100(Fe) packed column gave a baseline separation of all the tested analytes with good precision. The separation was controlled by negative enthalpy change and entropy change in the reverse-phase mode, but positive enthalpy change and entropy change in the normal-phase mode. The relative standard deviations of retention time, peak area, peak height, and half peak width for eleven replicate separations of the tested analytes were 0.2-0.7%, 0.5-3.6%, 0.6-2.3% and 0.8-1.7%, respectively. The mesoporous cages, accessible windows, excellent chemical and solvent stability, metal active sites and aromatic pore walls make MIL-100(Fe) a good candidate as a novel stationary phase for both normal-phase and reverse-phase high performance liquid chromatography.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, and Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Fu
authors.forename: Cheng-Xiong
authors.initials: CX
authors.lastname: Yang
authors.forename: Xiu-Ping
authors.initials: XP
authors.lastname: Yan
mesh_terms: D000814:Aniline Compounds / Q000302:isolation & purification; D001554:Benzene / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D054879:Halogenation; D058085:Iron Compounds / Q000737:chemistry*; D007536:Isomerism; D009281:Naphthalenes / Q000302:isolation & purification; D016062:Porosity; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013816:Thermodynamics; D014050:Toluene / Q000302:isolation & purification
chemical_list: D000814:Aniline Compounds; D058085:Iron Compounds; D009281:Naphthalenes; D014050:Toluene; D001554:Benzene