title: Characterization of membrane-associated glycoproteins using lectin affinity chromatography and mass spectrometry.
abstract: Membrane-associated glycoproteins play critical roles in many biological processes and are often the therapeutic targets for drug discovery. Lectin affinity chromatography is one of the most widely used approaches for enrichment of glycoproteins at the protein level. Here, we describe a strategy for the characterization of membrane glycoproteins including membrane protein extraction, lectin affinity chromatography, protein digestion, and analysis by LC-MS/MS.
authors.affiliation: Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI, USA.
authors.forename: Yashu
authors.initials: Y
authors.lastname: Liu
authors.forename: Jintang
authors.initials: J
authors.lastname: He
authors.forename: David M
authors.initials: DM
authors.lastname: Lubman
mesh_terms: D045744:Cell Line, Tumor; D002846:Chromatography, Affinity / Q000379:methods*; D002853:Chromatography, Liquid; D005909:Glioblastoma / Q000473:pathology; D006801:Humans; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000473:pathology; D019887:Peanut Agglutinin / Q000378:metabolism*; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000378:metabolism; D059748:Proteolysis; D012995:Solubility; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014357:Trypsin / Q000378:metabolism
chemical_list: D008562:Membrane Glycoproteins; D019887:Peanut Agglutinin; D014357:Trypsin; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase