title: Bacteriophage 434 Hex protein prevents recA-mediated repressor autocleavage.
abstract: In a λ(imm434) lysogen, two proteins are expressed from the integrated prophage. Both are encoded by the same mRNA whose transcription initiates at the P(RM) promoter. One protein is the 434 repressor, needed for the establishment and maintenance of lysogeny. The other is Hex which is translated from an open reading frame that apparently partially overlaps the 434 repressor coding region. In the wild type host, disruption of the gene encoding Hex destabilizes λ(imm434) lysogens. However, the hex mutation has no effect on lysogen stability in a recA(-) host. These observations suggest that Hex functions by modulating the ability of RecA to stimulate 434 repressor autocleavage. We tested this hypothesis by identifying and purifying Hex to determine if this protein inhibited RecA‑stimulated autocleavage of 434 repressor in vitro. Our results show that in vitro a fragment of Hex prevents RecA-stimulated autocleavage of 434 repressor, as well as the repressors of the closely related phage P22. Surprisingly, Hex does not prevent RecA‑stimulated autocleavage of phage lambda repressor, nor the E. coli LexA repressor.
authors.affiliation: Department of Biological Sciences, University at Buffalo, Buffalo, NY 14260, USA. shkilnyj@gmail.com
authors.forename: Paul
authors.initials: P
authors.lastname: Shkilnyj
authors.forename: Michael P
authors.initials: MP
authors.lastname: Colon
authors.forename: Gerald B
authors.initials: GB
authors.lastname: Koudelka
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000821:virology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D008242:Lysogeny; D011499:Protein Processing, Post-Translational; D011938:Rec A Recombinases / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D012097:Repressor Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins; D011938:Rec A Recombinases