title: Synthesis, characterization, and cytotoxicity of TMC-graft-poly(vinyl alcohol) copolymers.
abstract: N-trimethyl chitosan-graft-poly(vinyl alcohol) (TMC-g-PVA) copolymers were prepared. The grafting reactions were conducted in water changing the feed ratios of poly(vinyl alcohol)/6-O-succinate-N-trimethyl chitosan (PVA/STMC). The structure of TMC-g-PVA copolymers was characterized through (1)H NMR spectroscopy, thermogravimetric analysis (TGA/DTG), wide-angle X-ray scattering (WAXS) and scanning electron microscopy (SEM). The quaternization degree (DQ) and substitution degree (DS) of N-trimethyl chitosan (TMC) and 6-O-succinate-N-trimethyl chitosan (STMC) were determined by (1)H NMR, being the spectroscopy 14.0 and 5.5mol-% found, respectively. The viability of HCT-116 cancerous cells was investigated at different concentrations. The effect of PVA/STMC ratios on the cytotoxicity of the TMC-g-PVA copolymers was examined and the CC50 values determined for every case.
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, GMPC, Departamento de Química, DBS Bloco B-08, Universidade Estadual de Maringá UEM, Av. Colombo 5790, CEP 87020-900 Maringá, Paraná, Brazil. Electronic address: afmartins50@yahoo.com.br.
authors.forename: Alessandro F
authors.initials: AF
authors.lastname: Martins
authors.forename: Pedro V A
authors.initials: PV
authors.lastname: Bueno
authors.forename: Heveline D M
authors.initials: HD
authors.lastname: Follmann
authors.forename: Samara R
authors.initials: SR
authors.lastname: Nocchi
authors.forename: Celso V
authors.initials: CV
authors.lastname: Nakamura
authors.forename: Adley F
authors.initials: AF
authors.lastname: Rubira
authors.forename: Edvani C
authors.initials: EC
authors.lastname: Muniz
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity; D003603:Cytotoxins / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D045325:HCT116 Cells; D006801:Humans; D015394:Molecular Structure; D011145:Polyvinyls / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity*; D012995:Solubility; D013329:Structure-Activity Relationship; D014867:Water / Q000737:chemistry
chemical_list: D003603:Cytotoxins; C000591621:N-trimethyl chitosan-graft-poly(vinyl alcohol); D011145:Polyvinyls; D014867:Water; D048271:Chitosan
keywords: Chitosan; Copolymers; Graft; N-trimethyl chitosan; Poly(vinyl alcohol)