title: The formation of occlusion-derived virus is affected by the expression level of ODV-E25.
abstract: Odv-e25 is a core gene of baculoviruses and encodes a 25.5 kDa protein located on both budded virus (BV) and occlusion-derived virus (ODV). Our previous study demonstrated that ODV-E25 was required for the formation of intranuclear microvesicles and ODV, and an odv-e25 deletion mutant could be rescued by re-expression of odv-e25 under its native promoter. To investigate the functions of ODV-E25 expression level on ODV formation, the promoter of ie-1 (pIE1), the odv-e25 native promoter, and the polyhedrin promoter (pPH) were used to direct odv-e25 expression. Our results showed that the production of ODV-E25 under its native promoter was higher than that under pIE1 but lower than that under pPH. Viral DNA replication and budded viruses (BVs) production showed that expression of odv-e25 under pIE1 and pPH could not completely repair the defects caused by the deletion of ODV-E25, while expression under its native promoter did. Electron microscopy showed that intranuclear microvesicles were found in all the constructs transfected cells except the odv-e25-null virus. However, mature ODVs only were detected in cells transfected with virus in which odv-e25 was expressed under its native or polyhedrin promoter. These results indicated that the formation occlusion-derived virus was affected by the expression level of ODV-E25.
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310029, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Hu
authors.forename: Xingwei
authors.initials: X
authors.lastname: Xiang
authors.forename: Shaofang
authors.initials: S
authors.lastname: Yu
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Wu
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000502:physiology*; D002467:Cell Nucleus / Q000648:ultrastructure / Q000821:virology; D017353:Gene Deletion; D015870:Gene Expression; D005816:Genetic Complementation Test; D008854:Microscopy, Electron; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D061987:Sf9 Cells; D018411:Spodoptera; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014771:Virion / Q000648:ultrastructure; D019065:Virus Assembly; D014779:Virus Replication
chemical_list: D014764:Viral Proteins