title: Apoptosis of periodontium cells in streptozototocin- and ligature-induced experimental diabetic periodontitis in rats.
abstract: OBJECTIVE
  The aim of this study was to investigate the presence of apoptosis of periodontium cells in streptozototocin- and ligature-induced experimental diabetic periodontitis in rats.
  
  
  METHODS
  Sixty-two 6-week-old male Sprague-Dawley (SD) rats were randomly divided into three groups: the diabetic periodontitis group (group DP; n = 22), periodontitis group (group P; n = 20) and normal control group (group N; n = 20). Diabetes was induced by intraperitoneal injection of streptozototocin (STZ). Periodontitis was initiated by ligating floss around maxillary second molars. The animals were sacrificed at 3 and 6 weeks after ligature placement in the P and DP groups. Maxillary dentoalveolar segments were isolated and were prepared for morphometric analysis of alveolar bone loss (ABL) and for histological analysis.
  
  
  RESULTS
  ABL was significantly increased in group DP compared with group P (p < 0.05). The number of PDL fibroblasts, osteoblasts and osteocytes in group DP was decreased compared with group P (p < 0.05). Inter-group analysis revealed higher osteoclast numbers in the inflammatory area of group DP and group P when compared with group N (p < 0.05). Also, compared with group P, group DP had more higher osteoclast numbers (p < 0.05). Periodontitis and diabetic periodontitis also increased apoptosis of fibroblasts, osteoblasts and osteocytes. The percentage of these apoptotic cells was ∼ 2-fold higher in group DP vs group P.
  
  
  CONCLUSIONS
  The results of these studies suggest that diabetes may have a negative effect on the periodontium by increasing the formation of osteoclasts and enhancing apoptosis of fibroblasts, osteoblasts and osteocytes in the periodontal tissue.
authors.affiliation: Department of Stomatology, The First People's Hospital of Lianyungang City, Lianyungang, Jiangsu Province, PR China. fuyongweivip@sina.com
authors.forename: Yong-Wei
authors.initials: YW
authors.lastname: Fu
authors.forename: Hong-Bing
authors.initials: HB
authors.lastname: He
mesh_terms: D000818:Animals; D017209:Apoptosis; D003921:Diabetes Mellitus, Experimental / Q000473:pathology*; D020287:In Situ Nick-End Labeling; D008297:Male; D010518:Periodontitis / Q000209:etiology / Q000473:pathology*; D010519:Periodontium / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin
chemical_list: D013311:Streptozocin