title: Gross karyotypic and phenotypic alterations among different progenies of the Candida glabrata CBS138/ATCC2001 reference strain.
abstract: Genomic plasticity is a mechanism for adaptation to environmental cues such as host responses and antifungal drug pressure in many fungi including the human pathogenic yeast Candida glabrata. In this study we evaluated the phenotypic and genotypic stability of the world-wide used C. glabrata reference strain CBS138/ATCC2001 under laboratory conditions. A set of ten lineages of this wild type strain and genetically modified progenies were obtained from different scientific laboratories, and analyzed for genotypic and phenotypic alterations. Even though the derivates were indistinguishable by multi locus sequence typing, different phenotypic groups that correlated with specific karyotypic changes were observed. In addition, modifications in the adherence capacity to plastic surface emerged that were shown to correlate with quantitative changes in adhesin gene expression rather than subtelomeric gene loss or differences in the number of macrosatellite repeats within adhesin genes. These results confirm the genomic plasticity of C. glabrata and show that chromosomal aberrations and functional adaptations may occur not only during infection and under antimicrobial therapy, but also under laboratory conditions without extreme selective pressures. These alterations can significantly affect phenotypic properties such as cell surface attributes including adhesion and the cell wall carbohydrate composition and therefore, if unnoticed, may adulterate the outcome of genetic studies.
authors.affiliation: Institute for Medical Microbiology and German National Reference Center for Systemic Mycoses, University Medical Center Göttingen, Göttingen, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Bader
authors.forename: Alexander
authors.initials: A
authors.lastname: Schwarz
authors.forename: Eefje A
authors.initials: EA
authors.lastname: Kraneveld
authors.forename: Marut
authors.initials: M
authors.lastname: Tangwattanachuleeporn
authors.forename: Marut
authors.initials: M
authors.lastname: Tangwattanchuleeporn
authors.forename: Pia
authors.initials: P
authors.lastname: Schmidt
authors.forename: Mette D
authors.initials: MD
authors.lastname: Jacobsen
authors.forename: Uwe
authors.initials: U
authors.lastname: Gross
authors.forename: Piet W J
authors.initials: PW
authors.lastname: De Groot
authors.forename: Michael
authors.initials: M
authors.lastname: Weig
mesh_terms: D041221:Candida glabrata / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics; D016681:Genome, Fungal / Q000235:genetics; D007621:Karyotyping
chemical_list: D005656:Fungal Proteins