title: Simultaneous infection with distinct strains of torque teno sus virus (TTSuV) in healthy slaughter-age pigs.
abstract: The aims of this study were to evaluate torque teno sus virus (TTSuV) infection in healthy slaughter-age pigs and to compare the similarities of the untranslated region (UTR) nucleotide (nt) sequences obtained from different biological samples of the same animals. Fifty-eight pigs were evaluated by PCR assay for the presence of TTSuV in paired samples of liver and serum (Group 1, n = 27) and lung and serum (Group 2, n = 31). All the pigs were positive for TTSuV infection in the organs sampled and 94.8 % (n = 55) presented with viraemia. The nt sequence similarities between the Groups 1 and 2 varied from 91.7 % to 96.6 % (TTSuV1) and 91 % to 95 % (TTSuV2). In Group 1, the nt sequence similarities were 93 % (TTSuV1) and 95.4 % (TTSuV2). In Group 2, the nt sequence similarities were 95 % (TTSuV1) and 91 % (TTSuV2). These results revealed the simultaneous infection with distinct strains of TTSuV1 and 2 in healthy pigs at slaughter age.
authors.affiliation: Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, PR, Brazil.
authors.forename: Raquel
authors.initials: R
authors.lastname: de Arruda Leme
authors.forename: Juliane
authors.initials: J
authors.lastname: Ribeiro
authors.forename: Alice Fernandes
authors.initials: AF
authors.lastname: Alfieri
authors.forename: Amauri Alcindo
authors.initials: AA
authors.lastname: Alfieri
mesh_terms: D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D008099:Liver / Q000821:virology*; D008168:Lung / Q000821:virology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA; D013552:Swine / Q000097:blood / Q000821:virology*; D022783:Torque teno virus / Q000235:genetics / Q000302:isolation & purification*; D020506:Untranslated Regions
chemical_list: D004279:DNA, Viral; D020506:Untranslated Regions