title: [Simultaneous extraction and determination of nine chemically synthetic anticoccidial drug multi-residues in chicken meat using high performance liquid chromatography-tandem mass spectrometry].
abstract: A high performance liquid chromatography-tandem mass spectrometric (HPLC-MS/ MS) method was developed for the simultaneous extraction and determination of multi-residues of nine chemically synthetic anticoccidial drugs in chicken meat. Toltrazuril-D3 was used as the internal standard. The sample was extracted by 15 mL acetonitrile/dimethyl sulfoxide (4:1, v/ v, containing 2% acetic acid), cleaned-up by a QuEChERS (quick, easy, cheap, effective, rugged and safe) clean-up tube with 150 mg octadecylsilyl (ODS), 100 mg Florisil and 100 mg graphitized carbon black (GCB). After the concentration, the extract was analyzed by HPLC-MS/MS. The results showed that good linearity in the range of 20 - 150 microg/L for each target compound. The recoveries were between 81.5% and 103. 6% with the relative standard deviations lower than 15%. The method is simple, rapid, and suitable to detect the chemically synthetic anticoccidial drug residues with high throughput.
authors.affiliation: Poultry Institute, Chinese Academy of Agricultural Sciences/Supervision, Inspection and Testing Center of Poultry Quality, Ministry of Agriculture Yangzhou, Yangzhou 225125, China.
authors.forename: Zuhao
authors.initials: Z
authors.lastname: Shi
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhang
authors.forename: Shijin
authors.initials: S
authors.lastname: Bu
authors.forename: Dawei
authors.initials: D
authors.lastname: Chen
authors.forename: Junhua
authors.initials: J
authors.lastname: Pu
authors.forename: Yushi
authors.initials: Y
authors.lastname: Gao
authors.forename: Xiujun
authors.initials: X
authors.lastname: Tang
mesh_terms: D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003049:Coccidiostats / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D008461:Meat Products / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D003049:Coccidiostats