title: [Activation of lymphocytes under the influence of an influenza vaccine combined with a low molecular weight germanium organic compound].
abstract: OBJECTIVE
  Confirmation of immunostimulating effect of an original low molecular weight germanium organic compound (LMW-GOC) during immunization of mice with Vaxigrip vaccine.
  
  
  METHODS
  The experiments were carried out in CBA mice divided into 4 groups: control, those that received Vaxigrip influenza vaccine intraperitoneally, those that received LMW-GOC intraperitoneally and those that received both preparations at once. Effect of the preparations administered was evaluated by flow cytofluorometry based on changes of CD3, CD4, CD5, CD8, CD19, CD25, Foxp3, NK1.1, gammadelta T, MHC II, TLR2, TLR4, TLR9 expressing cell content in mice spleens. The content of the colored cells was determined at normal, 24 hours and 7 days after the administration of the preparations. Statistical treatment of the data was carried out by using Win MD 128 program package.
  
  
  RESULTS
  LMW-GOC can enhance the effect of Vaxigrip vaccine that is expressed by an increase of content in spleen of some lymphocyte subpopulations 24 hours and 7 days after the intraperitoneal administration. In some cases LMW-GOC increases the content of some lymphocyte subpopulations in mice spleen after administration as a monopreparation, i.e. without the vaccine. LMW-GOC suppressed stimulating effect of the vaccine on the spleen content of various lymphocyte subpopulations in none of the observations.
  
  
  CONCLUSIONS
  By using cytofluorometry method it is possible to form an understanding of an elevated role of various types of cells in the development of immune response to the vaccine as well as regarding additional enhancement of this response during administration of LMW-GOC to mice. The effect of the preparation is manifested for a few days after its administration. The preparation manifests adjuvant properties and after further studies may be suggested for use as an adjuvant.
authors.forename: V A
authors.initials: VA
authors.lastname: Liashenko
authors.forename: N K
authors.initials: NK
authors.lastname: Akhmatova
authors.forename: I V
authors.initials: IV
authors.lastname: Ambrosov
authors.forename: S K
authors.initials: SK
authors.lastname: Matelo
authors.forename: É A
authors.initials: ÉA
authors.lastname: Akhmatov
authors.forename: A S
authors.initials: AS
authors.lastname: Sukhno
authors.forename: V G
authors.initials: VG
authors.lastname: Khomenkov
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage* / Q000737:chemistry; D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D005434:Flow Cytometry; D005857:Germanium / Q000737:chemistry / Q000276:immunology*; D016130:Immunophenotyping; D007252:Influenza Vaccines / Q000008:administration & dosage* / Q000276:immunology; D008213:Lymphocyte Activation; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008970:Molecular Weight; D009942:Organometallic Compounds / Q000008:administration & dosage* / Q000737:chemistry; D009975:Orthomyxoviridae / Q000187:drug effects / Q000276:immunology; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D051193:Toll-Like Receptors / Q000276:immunology; D014611:Vaccination; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D015703:Antigens, CD; D007252:Influenza Vaccines; D009942:Organometallic Compounds; D051193:Toll-Like Receptors; D015164:Vaccines, Inactivated; C478242:vaxigrip; D005857:Germanium