title: [Regulatory effects of cyclosporin A and tacrolimus on the immunological gene expressions in renal transplant recipients].
abstract: OBJECTIVE
  To observe the change of Th immunological gene in renal transplant recipients after the treatment of cyclosporine (CsA) and tacrolimus (FK506).
  
  
  METHODS
  The peripheral blood lymphacytes just before and 24 hours after CsA and FK506 treatment were isolated. The total RNA of them were reverse-transcripted and examined by real-time quantity PCR array. The results were analyzed by bioinformatic methods.
  
  
  RESULTS
  The TLR4, CEBPB, IL4R, IL1R1,IL18R1,and IL1R2 genes were remarkably upregulated, whereas IL-2, CCL5, CD27, CCR5, CCR4, CD4, RPL13A, TGFB3, CD86, CCR3, STAT1, NFATC2IP, IL23A, IL15, IRF4, and TFCP2 were downregulated 24 hours after CsA treatment. The IL18, IL7, PTPRC, TNFSF4, SPP1, GFI1, TLR4, IL13RA1, TNF, INHBA, LAG3, IL13, IL1R1, SOCS5, IL10, YY1, TBX21, FASLG, IL18R1, and IL1R2 genes were remarkably upregulated, whereas IL-2, IL-3, IL-4, IL-6,CCR5, CD4, CD27, CD40LG, IL15, CCR3, CD86, CCR4, and IRF4 were obviously downregulated 24 hours after FK506 treatment.
  
  
  CONCLUSIONS
  CsA and FK506 exert their therapeutic effectiveness by regulating the expressions of a series of target genes.
authors.affiliation: Department of Urology, PUMC Hospital, CAMS and PUMC, Beijing, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wen
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Ji
authors.forename: Ji-rui
authors.initials: JR
authors.lastname: Niu
mesh_terms: D016572:Cyclosporine / Q000494:pharmacology*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D016030:Kidney Transplantation; D020411:Oligonucleotide Array Sequence Analysis; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects* / Q000378:metabolism; D016559:Tacrolimus / Q000494:pharmacology*
chemical_list: D016207:Cytokines; D016572:Cyclosporine; D016559:Tacrolimus