title: Protective effect of sesaminol from Sesamum indicum Linn. against oxidative damage in PC12 cells.
abstract: Sesaminol is one component of sesame oil and has been widely used as the stabilizer to extend the storage period of food oil in China. In this study, we tried to investigate the antioxidant activity of sesaminol on rat pheochromocytoma (PC12) cells oxidative damaged by H2 O2 . Cell viability, LDH level and apoptosis of the PC12 cells were assayed after treatment with sesaminol for 3 h and exposure to H2 O2 . Furthermore, superoxide (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and intracellular ROS were assayed after exposure of the PC12 cells to H2 O2 . The results showed that pre-treatment with sesaminol prior to H2 O2 exposure significantly elevated cell survival rate and SOD, CAT and GSH-Px activity. Meanwhile, sesaminol declined the secreted LDH level, apoptosis rate and ROS level of H2 O2 exposed cells. Thus, sesaminol may protect PC12 against oxidative injury.
authors.affiliation: Shanghai Grain Science Research Institute, Shanghai, China.
authors.forename: Wenming
authors.initials: W
authors.lastname: Cao
authors.forename: Mingjun
authors.initials: M
authors.lastname: Dai
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.forename: Fengjiao
authors.initials: F
authors.lastname: Yuan
authors.forename: Fengxiang
authors.initials: F
authors.lastname: Chen
authors.forename: Weiyun
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004149:Dioxoles / Q000494:pharmacology*; D005663:Furans / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000494:pharmacology; D031685:Sesamum / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D004149:Dioxoles; D005663:Furans; D017382:Reactive Oxygen Species; C066016:sesaminol; D006861:Hydrogen Peroxide
keywords: H2O2; PC12 cell; anti-apoptosis; antioxidation; sesaminol