title: [Importance of polymerase chain reaction in patients with histopathological diagnosis of granulomatous disease by EBUS-TBNA: a preliminary report].
abstract: BACKGROUND
  Transbronchial needle aspiration (TBNA) by the guide of endobronchial ultrasonography (EBUS) is a diagnostic method which may be used in diagnosing benign granulomatous diseases in contemporary practice. In this study, we planned to evaluate the diagnostic value of TBNA by the guide of EBUS for benign granulamatous diseases and to investigate the impact of cytological, microbiologic and tuberculosis (TB)-polimerase chain reaction (PCR) results in specimens.
  
  
  METHODS
  Thirty-four patients (20 female, 14 male) who had EBUS-TBNA and histopathological diagnosis of granülamatous inflammatory reaction were included in the study. The mean age of patients was 49.62 ± 12.72 years (26-72). The specimens provided by EBUS-TBNA were evaluated for pathologic, microbiologic, and molecular (TB-PCR) examinations.
  
  
  RESULTS
  As a result of clinical, radiological and laboratory studies of the patients with granulomatous inflammation, 13 (38.2%) patients had TB, 21 (61.7%) patients had sarcoidosis. Diagnoses of histopathological granuloma were established by EBUS-TBNA in 30 (88.2%) patients among patients with isolated mediastinal lymphadenopathies. Seven of mediastinal TB lymphadenopathies patients were TB-PCR positive. The sensitivity of PCR was 54% and the specificity was 100%, general efficiency of the test was found to be 82%.
  
  
  CONCLUSIONS
  In cases with granulomatosis lymphadenopathies, TB-PCR specimens which achieved by EBUS-TBNA results provided important contribution for the TB diagnosis. Therefore, we thought that TB-PCR is a diagnostic method to be studied in the cases who investigating for mediastinal lymphadenopathies.
authors.affiliation: Clinic of Chest Diseases, Ankara Ataturk Training and Research Hospital, Ankara, Turkey. asenturk1967@yahoo.com
authors.forename: Ayşegül
authors.initials: A
authors.lastname: Sentürk
authors.forename: Habibe
authors.initials: H
authors.lastname: Hezer
authors.forename: Ayşegül
authors.initials: A
authors.lastname: Karalezli
authors.forename: Emine
authors.initials: E
authors.lastname: Argüder
authors.forename: Hatice
authors.initials: H
authors.lastname: Kılıç
authors.forename: H Canan
authors.initials: HC
authors.lastname: Hazanoğlu
mesh_terms: D000328:Adult; D000368:Aged; D001707:Biopsy, Needle / Q000379:methods*; D003937:Diagnosis, Differential; D005260:Female; D006099:Granuloma / Q000175:diagnosis / Q000473:pathology; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis / Q000473:pathology; D008199:Lymphadenitis / Q000175:diagnosis / Q000473:pathology; D008206:Lymphatic Diseases / Q000175:diagnosis / Q000473:pathology; D008297:Male; D008477:Mediastinal Diseases / Q000175:diagnosis / Q000473:pathology; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D015203:Reproducibility of Results; D017565:Sarcoidosis, Pulmonary / Q000175:diagnosis / Q000473:pathology; D012680:Sensitivity and Specificity; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000473:pathology