title: [The application of polymerase chain reaction in real-time operation mode to detect DNA of agents of human granulocytic anaplasmosis and monocytic erlychiosis].
abstract: The analysis was applied to detect DNA of agents of human granulocytic anaplasmosis and monocytic erlychiosis. The sampling included 109 ticks of Ixodes species from Novosibirsk oblast and Khabarovsk kray and blood samples of 111 mouse-like rodents from Omsk oblast. The used techniques included polymerase chain reaction in real-time operation mode with set of reagents "RealBest DNA Anaplasma phagocytophilum/Ehrlichia muris, ehrlichia chaffeensis" ("Vector-Best" Novosibirsk) and double round polymerase chain reaction. The DNA of A. phagocytophilum, agent of granulocytic anaplasmosis and/or DNA of E. muris, agent of monocytic erlychiosis was detected in 21 ticks and in blood samples of 52 voles. Both techniques were applied. The DNA of A. phagocytophilum was detected in samples of 2 voles and in 1 tick only after polymerase chain reaction in real-time operation mode was applied. It demonstrated that the set of reagents "RealBest DNA Anaplasma phagocytophilum/Ehrlichia muris, ehrlichia chaffeensis" permits to detect the DNA of isolates of A. phagocytophilum subsumed to different genetic groups. The set can be used for fast and effective detection of the DNA of agents of agents of human granulocytic anaplasmosis and monocytic erlychiosis in suspensions of analyzed ticks and blood samples.
authors.forename: E I
authors.initials: EI
authors.lastname: Bondarenko
authors.forename: M K
authors.initials: MK
authors.lastname: Ivanov
authors.forename: V V
authors.initials: VV
authors.lastname: Iakimenko
authors.forename: A K
authors.initials: AK
authors.lastname: Tantsev
authors.forename: V V
authors.initials: VV
authors.lastname: Panov
authors.forename: T I
authors.initials: TI
authors.lastname: Epikhina
authors.forename: V A
authors.initials: VA
authors.lastname: Rar
mesh_terms: D041081:Anaplasma phagocytophilum / Q000235:genetics / Q000302:isolation & purification; D000710:Anaplasmataceae / Q000235:genetics / Q000302:isolation & purification*; D000712:Anaplasmosis / Q000097:blood / Q000382:microbiology*; D000818:Animals; D003411:Arvicolinae / Q000097:blood; D004269:DNA, Bacterial / Q000032:analysis* / Q000097:blood; D004536:Ehrlichia / Q000235:genetics / Q000302:isolation & purification; D016873:Ehrlichiosis / Q000097:blood / Q000382:microbiology*; D006801:Humans; D018884:Ixodes / Q000382:microbiology*; D051189:Murinae / Q000097:blood; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012800:Siberia
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S