title: Studying the activation of epithelial ion channels using global whole-field photolysis.
abstract: The production of saliva by parotid acinar cells is stimulated by Ca(2+) activation of Cl(-) and K(+) channels located in the apical plasma membrane of these polarized cells. Here we provide a detailed description of a flash photolysis experiment designed to give a global and relatively uniform photorelease of inositol 1,4,5-trisphosphate (InsP(3)) or Ca(2+) from caged precursors (NPE-InsP(3) or NP-EGTA) combined with the simultaneous measurement of whole-cell Ca(2+)-activated currents. The photolysis light source can be either an ultraviolet (UV) flash lamp or alternatively the output from a 375-nm diode laser, which is defocused to illuminate the entire field.
authors.affiliation: Department of Pharmacology and Physiology, University of Rochester, Rochester, NY 14642, USA.
authors.forename: Janos
authors.initials: J
authors.lastname: Almassy
authors.forename: David I
authors.initials: DI
authors.lastname: Yule
mesh_terms: D061354:Acinar Cells / Q000502:physiology* / Q000528:radiation effects; D000818:Animals; D002118:Calcium / Q000032:analysis; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000379:methods*; D015544:Inositol 1,4,5-Trisphosphate / Q000032:analysis; D007473:Ion Channels / Q000378:metabolism* / Q000528:radiation effects; D008027:Light; D051379:Mice; D010782:Photolysis; D014466:Ultraviolet Rays
chemical_list: D007473:Ion Channels; D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium