title: A rapid and sensitive liquid chromatography-tandem mass spectrometric assay for cycloserine in 50μL of human plasma: Its pharmacokinetic application.
abstract: This paper describes a simple, rapid and sensitive liquid chromatography/tandem mass spectrometry assay for the determination of cycloserine in human plasma using carbamazepine as internal standard (IS). Analyte and the IS were extracted from the 50μL of human plasma via protein precipitation using acetonitrile. The chromatographic separation was achieved on a C(18) column by using a mixture of acetonitrile-0.5% formic acid buffer (60:40, v/v) as the mobile phase at a flow rate of 0.8mL/min. The calibration curve obtained was linear (r(2)≥0.99) over the concentration range of 50-15,000ng/mL. Method validation was performed as per FDA guidelines and the results met the acceptance criteria. A run time of 2.5min for each sample made it possible to analyze more number of samples in short time, thus increasing the productivity. The proposed method was found to be applicable to pharmacokinetic studies.
authors.affiliation: Research Studies, Rayalaseema University, Kurnool 518 002, India; Wellquest Clinical Research, Ramanthapur, Hyderabad 500 013, India. srinu_polagani@yahoo.co.in
authors.forename: Srinivasa Rao
authors.initials: SR
authors.lastname: Polagani
authors.forename: Nageswara Rao
authors.initials: NR
authors.lastname: Pilli
authors.forename: Rambabu
authors.initials: R
authors.lastname: Maddela
authors.forename: Ramakrishna
authors.initials: R
authors.lastname: Gajula
authors.forename: Venkateswarlu
authors.initials: V
authors.lastname: Gandu
mesh_terms: D000904:Antibiotics, Antitubercular / Q000097:blood*; D002138:Calibration; D002220:Carbamazepine / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003523:Cycloserine / Q000097:blood*; D017408:Guidelines as Topic; D006801:Humans; D008297:Male; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors; D014481:United States; D014486:United States Food and Drug Administration
chemical_list: D000904:Antibiotics, Antitubercular; D002220:Carbamazepine; D003523:Cycloserine