title: Evaluation of the MYC_CANFA gene in Chinese Shar Peis with cobalamin deficiency.
abstract: BACKGROUND
  A recent genome-wide scan using the canine minimal screening set 2 (MSS-2) showed that cobalamin deficiency appears to be hereditary in Chinese Shar Peis and is linked to the microsatellite markers DTR13.6 and REN13N11 on canine chromosome 13.
  
  
  OBJECTIVE
  The goal of this study was to evaluate the MYC_CANFA gene, which is the closest known gene with a distance of approximately 0.06 megabases (Mb) to the microsatellite marker DTR13.6, for any mutations in this breed.
  
  
  METHODS
  Microsatellite markers (Myc and G15987) for genotyping and primers for sequencing were used to evaluate the MYC_CANFA gene. The genotype and gene sequence were compared between cobalamin-deficient Shar Peis, Shar Peis with normal serum cobalamin concentrations, and the DNA sequences published as part of the Ensemble Genomic map.
  
  
  RESULTS
  Neither the microsatellite markers (Myc and G15987) nor the sequences of the MYC_CANFA gene showed a significant difference among both groups of Shar Peis and the published canine DNA sequence.
  
  
  CONCLUSIONS
  The data presented here suggest that cobalamin deficiency in Shar Peis is not related to any mutations of the MYC_CANFA gene according to the genotyping and sequencing results in this study. Further investigations are warranted to find a potential genomic locus in proximity to DTR13.6 and REN13N11 that shows mutations in cobalamin-deficient Shar Peis.
authors.affiliation: Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA. ngruetzner@cvm.tamu.edu
authors.forename: Niels
authors.initials: N
authors.lastname: Grützner
authors.forename: Micah A
authors.initials: MA
authors.lastname: Bishop
authors.forename: Jan S
authors.initials: JS
authors.lastname: Suchodolski
authors.forename: Jörg M
authors.initials: JM
authors.lastname: Steiner
mesh_terms: D000483:Alleles; D000818:Animals; D014408:Biomarkers, Tumor; D018076:DNA, Complementary / Q000737:chemistry; D004283:Dog Diseases / Q000235:genetics* / Q000473:pathology; D004285:Dogs; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D018895:Microsatellite Repeats; D017422:Sequence Analysis, DNA / Q000662:veterinary; D014806:Vitamin B 12 Deficiency / Q000235:genetics / Q000662:veterinary*
chemical_list: D014408:Biomarkers, Tumor; D018076:DNA, Complementary