title: Ragweed pollen extract intensifies lipopolysaccharide-induced priming of NLRP3 inflammasome in human macrophages.
abstract: Ragweed pollen extract (RWE) possesses intrinsic NADPH oxidase activity that induces oxidative stress by initiating the production of intracellular reactive oxygen species (ROS). The ROS are important contributors to the manifestation of allergic inflammation; furthermore, concomitant exposure to an allergen and an endotoxin trigger a stronger inflammatory response. One of the main pro-inflammatory cytokines produced in inflammatory responses is interleukin-1β (IL-1β), and its production is associated with caspase-1-containing inflammasome complexes. Intracellular ROS have been implicated in NLRP3 inflammasome-mediated IL-1β production, therefore, we aimed to study whether RWE influences the function of NLRP3 inflammasome. Here we describe that, in the presence of NADPH, RWE significantly elevates lipopolysaccharide-induced IL-1β production of THP-1 cells as well as human primary macrophages and dendritic cells. We also demonstrate that increased IL-1β production is mediated through NLRP3 inflammasome in THP-1 macrophages. We provide evidence that RWE elevates cytosolic ROS level in these cells, and ROS inhibitors abolish IL-1β production. Furthermore, we show that RWE enhances lipopolysaccharide-induced gene transcription/expression of pro-IL-1β and key components of the inflammasome via a ROS-dependent mechanism.
authors.affiliation: Department of Immunology, Medical and Health Science Centre, University of Debrecen, Debrecen, Hungary.
authors.forename: Aliz
authors.initials: A
authors.lastname: Varga
authors.forename: Marietta M
authors.initials: MM
authors.lastname: Budai
authors.forename: Sándor
authors.initials: S
authors.lastname: Milesz
authors.forename: Attila
authors.initials: A
authors.lastname: Bácsi
authors.forename: József
authors.initials: J
authors.lastname: Tőzsér
authors.forename: Szilvia
authors.initials: S
authors.lastname: Benkő
mesh_terms: D052179:Antigens, Plant / Q000276:immunology / Q000494:pharmacology / Q000627:therapeutic use*; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology*; D020170:Caspase 1 / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D058847:Inflammasomes / Q000187:drug effects* / Q000235:genetics / Q000276:immunology; D007249:Inflammation / Q000276:immunology / Q000473:pathology; D053583:Interleukin-1beta / Q000235:genetics / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D009249:NADP / Q000378:metabolism / Q000494:pharmacology; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D010936:Plant Extracts / Q000276:immunology / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D052179:Antigens, Plant; D002352:Carrier Proteins; D016166:Free Radical Scavengers; D058847:Inflammasomes; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C453881:NLRP3 protein, human; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C433085:ragweed pollen; D009249:NADP; D020170:Caspase 1