title: SHORT INTERNODES/STYLISH genes, regulators of auxin biosynthesis, are involved in leaf vein development in Arabidopsis thaliana.
abstract: Leaves depend on highly developed venation systems to collect fixed carbon for transport and to distribute water. We hypothesized that local regulation of auxin biosynthesis plays a role in vein development. To this effect, we assessed the role of the SHORT INTERNODES/STYLISH (SHI/STY) gene family, zinc-finger transcription factors linked to regulation of auxin biosynthesis, in Arabidopsis thaliana leaf vein development. Gene functions were assessed by a combination of high-resolution spatio-temporal expression analysis of promoter-marker lines and phenotypic analysis of plants homozygous for single and multiple mutant combinations. The SHI/STY genes showed expression patterns with variations on a common theme of activity in incipient and developing cotyledon and leaf primordia, narrowing to apices and hydathode regions. Mutant analysis of single to quintuple mutant combinations revealed dose-dependent defects in vein patterning affecting multiple vein traits, most notably in cotyledons. Here we demonstrate that local regulation of auxin biosynthesis is an important aspect of leaf vein development. Our findings also support a model in which auxin synthesized at the periphery of primordia affects vein development.
authors.affiliation: Department of Biological Sciences, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada.
authors.forename: Tammy
authors.initials: T
authors.lastname: Baylis
authors.affiliation: Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Linnean Centre of Plant Biology in Uppsala, Swedish University of Agricultural Sciences, PO Box 7080, SE-750 07, Uppsala, Sweden.
authors.forename: Izabela
authors.initials: I
authors.lastname: Cierlik
authors.affiliation: Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Linnean Centre of Plant Biology in Uppsala, Swedish University of Agricultural Sciences, PO Box 7080, SE-750 07, Uppsala, Sweden.
authors.forename: Eva
authors.initials: E
authors.lastname: Sundberg
authors.affiliation: Department of Biological Sciences, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada.
authors.forename: Jim
authors.initials: J
authors.lastname: Mattsson
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005786:Gene Expression Regulation; D007210:Indoleacetic Acids / Q000378:metabolism* / Q000494:pharmacology; D016297:Mutagenesis, Site-Directed; D010937:Plant Growth Regulators / Q000378:metabolism*; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016335:Zinc Fingers
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D014157:Transcription Factors; C120562:short internodes protein, Arabidopsis