title: Synthesis and in vitro evaluation of MR molecular imaging probes using J591 mAb-conjugated SPIONs for specific detection of prostate cancer.
abstract: Carcinoma of the prostate is the most frequent diagnosed malignant tumor in men and is the second leading cause of cancer-related death in this group. The cure rate of prostate cancer is highly dependent on the stage of disease at the diagnosis and early detection is key to designing effective treatment strategies. The objective of the present study is to make a specific MR imaging probe for targeted imaging of cancer cells. We take advantage of the fact that many types of prostate cancer cells express high levels of prostate-specific membrane antigen (PSMA) on their cell surface. The imaging strategy is to use superparamagnetic iron oxide nanoparticles (SPIONs), attached to an antibody (J591) that binds to the extracellular domain of PSMA, to specifically enhance the contrast of PSMA-expressing prostate cancer cells. Conjugation of mAb J591 to commercial SPIONs was achieved using a heterobifunctional linker, sulfo-SMCC. Two types of prostate cancer cell lines were chosen for experiments: LNCaP (PSMA+) and DU145 (PSMA-). MRI and cell uptake experiments demonstrated the high potential of the synthesized nanoprobe as a specific MRI contrast agent for detection of PSMA-expressing prostate cancer cells.
authors.affiliation: Department of Medical Physics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Abdolahi
authors.forename: Daryoush
authors.initials: D
authors.lastname: Shahbazi-Gahrouei
authors.forename: Sophie
authors.initials: S
authors.lastname: Laurent
authors.forename: Corine
authors.initials: C
authors.lastname: Sermeus
authors.forename: Farzin
authors.initials: F
authors.lastname: Firozian
authors.forename: Barry J
authors.initials: BJ
authors.lastname: Allen
authors.forename: Sebastien
authors.initials: S
authors.lastname: Boutry
authors.forename: Robert N
authors.initials: RN
authors.lastname: Muller
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D045744:Cell Line, Tumor; D003287:Contrast Media; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles; D008297:Male; D015336:Molecular Probe Techniques; D015335:Molecular Probes; D017430:Prostate-Specific Antigen / Q000276:immunology*; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000276:immunology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D003287:Contrast Media; C478680:J591 monoclonal antibody; D058185:Magnetite Nanoparticles; D015335:Molecular Probes; D017430:Prostate-Specific Antigen