title: Characterization of an α-L-Rhamnosidase from Streptomyces avermitilis.
abstract: The putative α-L-rhamnosidase gene from Streptomyces avermitilis was cloned and expressed. The recombinant enzyme released L-rhamnose from p-nitrophenyl α-L-rhamnoside, Citrus flavonoids such as naringin, rutin, and hesperidin, and gum arabic which is an arabinogalactan-protein. Calcium ions increased L-rhamnose production by the enzyme from gum arabic, whereas enzyme activity was not affected by any metal ions.
authors.affiliation: Food Biotechnology Division, National Food Research Institute, Tsukuba, Ibaraki, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Ichinose
authors.forename: Zui
authors.initials: Z
authors.lastname: Fujimoto
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kaneko
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D002118:Calcium / Q000737:chemistry; D002413:Cations, Divalent; D003001:Cloning, Molecular; D057075:Enzyme Assays; D004926:Escherichia coli / Q000235:genetics; D044950:Flavanones / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000235:genetics; D006170:Gum Arabic / Q000737:chemistry; D006569:Hesperidin / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012210:Rhamnose / Q000737:chemistry*; D012431:Rutin / Q000737:chemistry; D013302:Streptomyces / Q000737:chemistry / Q000201:enzymology*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D002413:Cations, Divalent; D044950:Flavanones; D011994:Recombinant Proteins; D012431:Rutin; D006170:Gum Arabic; D006569:Hesperidin; D006026:Glycoside Hydrolases; C022525:alpha-L-rhamnosidase; C005274:naringin; D012210:Rhamnose; D002118:Calcium