title: Ganglioside-embedding small bicelles for probing membrane-landing processes of intrinsically disordered proteins.
abstract: We successfully displayed a series of gangliosides on small bicelles with a uniform confined size, offering nanoscale standardized membrane mimics for spectroscopic characterization of weak encounter complexes formed between ganglioside clusters and amyloidogenic proteins. This enabled probing of initial membrane-landing processes of α-synuclein as a therapeutic target by NMR spectroscopy.
authors.affiliation: Institute for Molecular Science and Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, 5-1 Higashiyama Myodaiji, Okazaki 444-8787, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Uno
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Uekusa
authors.forename: Maho
authors.initials: M
authors.lastname: Yagi-Utsumi
authors.forename: Koichi
authors.initials: K
authors.lastname: Kato
mesh_terms: D002942:Circular Dichroism; D005677:G(M1) Ganglioside / Q000737:chemistry; D005678:G(M2) Ganglioside / Q000737:chemistry; D005732:Gangliosides / Q000737:chemistry*; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D011485:Protein Binding; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D051844:alpha-Synuclein / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D005732:Gangliosides; D008051:Lipid Bilayers; D011994:Recombinant Proteins; D051844:alpha-Synuclein; D005678:G(M2) Ganglioside; D005677:G(M1) Ganglioside