title: B7 costimulation and intracellular indoleamine-2,3-dioxygenase expression in peripheral blood of healthy pregnant and pre-eclamptic women.
abstract: OBJECTIVE
  We determined the frequency of activated (CD11b+) monocytes expressing B7-1, B7-2, B7-H1, and B-7H2, and that of T cells and T helper cells expressing CD28, CTLA-4, PD-1, and ICOS in peripheral blood samples from normal pregnant (NP) and pre-eclamptic (PE) women. We also examined the intracellular expression of indoleamine-2,3-dioxygenase (IDO).
  
  
  METHODS
  We measured the expression of the above markers using flow-cytometry in peripheral blood samples from 20 NP and 20 PE women in the third trimester.
  
  
  RESULTS
  The frequency of B7-1 and B7-2 expressing activated monocytes and that of IDO expressing T-lymphocytes was lower in PE than in NP.
  
  
  CONCLUSIONS
  Lower expression of B7-1 and B7-2 proteins on peripheral monocytes in PE might indicate a secondary regulatory mechanism in response to the ongoing systemic maternal inflammation. IDO plays an important role in the pregnancy-specific immune tolerance, and might be a contributing factor in the pathogenesis of PE.
authors.affiliation: First Department of Pediatrics, Semmelweis University, Budapest, Hungary. toldigergely@yahoo.com
authors.forename: Gergely
authors.initials: G
authors.lastname: Toldi
authors.forename: Barna
authors.initials: B
authors.lastname: Vásárhelyi
authors.forename: Enikő
authors.initials: E
authors.lastname: Biró
authors.forename: Gergely
authors.initials: G
authors.lastname: Fügedi
authors.forename: János
authors.initials: J
authors.lastname: Rigó
authors.forename: Attila
authors.initials: A
authors.lastname: Molvarec
mesh_terms: D018122:B7-1 Antigen / Q000378:metabolism*; D051940:B7-2 Antigen / Q000378:metabolism*; D039481:CD11b Antigen / Q000378:metabolism; D018106:CD28 Antigens / Q000378:metabolism; D060908:CTLA-4 Antigen / Q000378:metabolism; D005260:Female; D006801:Humans; D007108:Immune Tolerance; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000276:immunology / Q000378:metabolism*; D060889:Inducible T-Cell Co-Stimulator Protein / Q000378:metabolism; D042541:Intracellular Space / Q000378:metabolism*; D009000:Monocytes / Q000276:immunology*; D011225:Pre-Eclampsia / Q000276:immunology*; D011247:Pregnancy; D061026:Programmed Cell Death 1 Receptor / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; D039481:CD11b Antigen; D018106:CD28 Antigens; D060908:CTLA-4 Antigen; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D060889:Inducible T-Cell Co-Stimulator Protein; C105992:PDCD1 protein, human; D061026:Programmed Cell Death 1 Receptor