title: Bis-aryl methanone compound is a candidate of nitric oxide producing elicitor and induces resistance in Nicotiana benthamiana against Phytophthora infestans.
abstract: Nitric oxide (NO) is important in some physiological responses of plants and plays a crucial role in the regulation of both defense responses and inducing resistance to fungal pathogens. NUBS-4190, a new bis-aryl-methanone compound elicited NO production and defense responses in Nicotiana benthamiana against Phytophthora infestans. NUBS-4190 induced resistance in N. benthamiana to P. infestans, without association of reactive oxygen generation and hypersensitive cell death. Callose induction was reduced in NUBS-4190-treated N. benthamiana leaves after challenge inoculation of P. infestans indicating the penetration resistance. Involvement of pathogenesis-related 1a (NbPR1a) and nitric oxide associated 1 (NbNOA1) genes in the induced resistance to N. benthamiana against P. infestans was found to be associated with resistance. Increased susceptibility in NbPR1a- and NbNOA1-silenced plants correlated with the constitutive accumulation of PR1a transcripts and NO associated salicylic acid. Moreover, reduced NO generation in NOA1 silenced N. benthamiana plants treated with NUBS-4190 indicated that NbNOA1 is involved in NUBS-4190-mediated NO production and is required for defense responses.
authors.affiliation: Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors.forename: Mohammad Shahjahan
authors.initials: MS
authors.lastname: Monjil
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Shibata
authors.forename: Daigo
authors.initials: D
authors.lastname: Takemoto
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Kawakita
mesh_terms: D007555:Isoxazoles / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis*; D055750:Phytophthora infestans / Q000187:drug effects*; D010935:Plant Diseases / Q000469:parasitology*; D013450:Sulfones / Q000737:chemistry / Q000494:pharmacology*; D014026:Nicotiana / Q000187:drug effects* / Q000378:metabolism / Q000469:parasitology
chemical_list: D007555:Isoxazoles; D013450:Sulfones; D009569:Nitric Oxide