title: Preparation of an amperometric sensor for norfloxacin based on molecularly imprinted grafting photopolymerization.
abstract: A sensitive amperometric sensor for norfloxacin (NF) was introduced. The receptor layer was prepared by molecularly imprinted photopolymerization of acrylamide and trimethylolpropane trimethacrylate on the surface of a gold electrode. The binding mechanism of the molecularly imprinted polymer was explored by ultraviolet (UV) and infrared (IR) spectroscopy. The chemosensor was characterized by cyclic voltammetry (CV), differential pulse voltammetry (DPV), electrochemical impedance (EI), and scanning electron microscopy (SEM). The electrode prepared by photopolymerization has a better recognition ability to template molecules than that of electropolymerization and NIP. Some parameters affecting sensor response were optimized. Norfloxacin was detected by measurements of an amperometric i-t curve. The linear relationships between current and logarithmic concentration are obtained from 1.0 × 10(-9) to 1.0 × 10(-3) mol L(-1). The detection limit of the sensor was 1.0 × 10(-10) mol L(-1). The proposed method is sensitive, simple, and cheap, and is applied to detect NF in human urine successfully.
authors.affiliation: Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou, China. zhwang@nwnu.edu.cn
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Wang
authors.forename: Jinshu
authors.initials: J
authors.lastname: Li
authors.forename: Xiaole
authors.initials: X
authors.lastname: Liu
authors.forename: Jianming
authors.initials: J
authors.lastname: Yang
authors.forename: Xiaoquan
authors.initials: X
authors.lastname: Lu
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000652:urine; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D006801:Humans; D008027:Light; D054802:Molecular Imprinting; D009643:Norfloxacin / Q000032:analysis* / Q000652:urine; D058105:Polymerization / Q000528:radiation effects; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D011108:Polymers; D009643:Norfloxacin