title: Complete sequence of a bla(KPC-2)-harboring IncFII(K1) plasmid from a Klebsiella pneumoniae sequence type 258 strain.
abstract: We report the nucleotide sequence of a novel bla(KPC-2)-harboring IncFII(K1) plasmid, pBK32179, isolated from a carbapenem-resistant Klebsiella pneumoniae ST258 strain from a New York City patient. pBK32179 is 165 kb long, consists of a large backbone of pKPN3-like plasmid, and carries an 18.5-kb bla(KPC-2)-containing element that is highly similar to plasmid pKpQIL. pBK32179-like plasmids were identified in 8.3% of strains in a collection of 96 K. pneumoniae isolates from hospitals in the New York City area.
authors.affiliation: Public Health Research Institute Center, International Center for Public Health, University of Medicine and Dentistry of New Jersey, Newark, NJ, USA.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.forename: Kalyan D
authors.initials: KD
authors.lastname: Chavda
authors.forename: Roberto G
authors.initials: RG
authors.lastname: Melano
authors.forename: Michael R
authors.initials: MR
authors.lastname: Jacobs
authors.forename: Michael H
authors.initials: MH
authors.lastname: Levi
authors.forename: Robert A
authors.initials: RA
authors.lastname: Bonomo
authors.forename: Barry N
authors.initials: BN
authors.lastname: Kreiswirth
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D015780:Carbapenems / Q000494:pharmacology; D003428:Cross Infection / Q000188:drug therapy / Q000382:microbiology; D006801:Humans; D007710:Klebsiella Infections / Q000188:drug therapy / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009518:New York; D010957:Plasmids / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D001618:beta-Lactamases; C547585:beta-lactamase KPC-2, Klebsiella pneumoniae