title: Optimization of culture conditions for production of a novel cold-active lipase from Pichia lynferdii NRRL Y-7723.
abstract: Lipases with abnormal properties such as thermostability, alkalinity, acidity, and cold activity receive industrial attention because of their usability under restricted reaction conditions. Most microbial cold-active lipases originate from psychrotrophic and psychrophilic microorganisms found in Antarctic regions, which has led to difficulties in the practical production of cold-active lipase. Recently, a mesophilic yeast,  Pichia lynferdii  NRRL Y-7723, was reported to produce a novel cold-active lipase. This study focused on optimization of environmental factors, while giving particular attention to the relationships between given factors and incubation time, to maximize the production of a novel cold-active lipase from P. lynferdii NRRL Y-7723. Maximum lipase production was highly dependent on the incubation time at a given environmental factor. Lipase production varied with incubation time at a given temperature, and 20 °C was selected as the optimum temperature for lipase production. Fructose was selected as the best carbon source, and maximum lipase production was obtained when it was present at 0.7% (w/v). Yeast extract was an efficient organic nitrogen source, with maximum lipase production occurring at 0.9% (w/v). Specifically, at the optimum yeast extract level the lipase production was >10 times higher than the productivity under standard conditions. All natural oils tested showed lipase production, but their maximum productivities varied according to incubation time and oil species.
authors.affiliation: School of Food Science and Biotechnology, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Park
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Kim
authors.forename: Jae-Han
authors.initials: JH
authors.lastname: Bae
authors.forename: Ching T
authors.initials: CT
authors.lastname: Hou
authors.forename: Hak-Ryul
authors.initials: HR
authors.lastname: Kim
mesh_terms: D003080:Cold Temperature; D003470:Culture Media; D005632:Fructose; D008049:Lipase / Q000096:biosynthesis* / Q000378:metabolism; D010843:Pichia / Q000201:enzymology* / Q000254:growth & development*; D010938:Plant Oils; D013696:Temperature
chemical_list: D003470:Culture Media; D010938:Plant Oils; D005632:Fructose; D008049:Lipase