title: Antioxidant properties of cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide on human umbilical vein endothelial cells in vitro.
abstract: A new chemical component, cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide, was isolated from Angelica sinensis and its structure elucidated from its NMR and MS spectra and confirmed by X-ray single crystal diffraction analysis. We also explored the antioxidative properties of cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide on human umbilical vein endothelial cells (HUVECs) against injuries induced by hydrogen peroxide (H(2)O(2)) using an MTT assay and flow cytometry analysis. In addition, the activities of  superoxide dismutase (SOD), malondialdehyde (MDA), lactate dehydrogenase (LDH), nitric oxide (NO) and reactive oxygen species (ROS) were determined. We found that cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide increased the viability of HUVECs injured by H(2)O(2) in a dose-dependent manner, reduced the apoptosis of HUVEC, and enhanced HUVEC proliferation. Our results demonstrated the remarkable in vitro antioxidative activities of this compound, indicating that it could be a potential antioxidant with protective effects against H(2)O(2)-induced HUVEC injuries.
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Wu
authors.forename: Xuedong
authors.initials: X
authors.lastname: Liu
authors.forename: Cuiping
authors.initials: C
authors.lastname: Yan
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Pan
authors.forename: Guangming
authors.initials: G
authors.lastname: Yang
authors.forename: Fangzhou
authors.initials: F
authors.lastname: Yin
authors.forename: Zebin
authors.initials: Z
authors.lastname: Weng
authors.forename: Ding
authors.initials: D
authors.lastname: Zhao
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Chen
authors.forename: Baochang
authors.initials: B
authors.lastname: Cai
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000494:pharmacology; D029971:Angelica sinensis / Q000737:chemistry; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D018360:Crystallography, X-Ray; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects; D006575:Heterocyclic Compounds, 3-Ring / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D008958:Models, Molecular; D008968:Molecular Conformation; D009569:Nitric Oxide / Q000378:metabolism; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology
chemical_list: C581688:3a.7a',7a.3a'-dihydroxyligustilide; D000975:Antioxidants; D006575:Heterocyclic Compounds, 3-Ring; D016877:Oxidants; D010936:Plant Extracts; D014810:Vitamin E; D009569:Nitric Oxide; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase; D015107:4-Butyrolactone