title: Effect of the oral administration homeopathic Arnica montana on mitochondrial oxidative stress.
abstract: OBJECTIVE
  To analyze the effect of homeopathic Arnica on mitochondrial oxidative stress induced by Ca(2+) plus inorganic phosphate and/or Fe(2+)-citrate-mediated lipid peroxidation through changes in oxygen consumption rates.
  
  
  METHODS
  Mitochondria were isolated by differential centrifugation from the livers of adult male Wistar rats which had been treated with Arnica montana 6cH, 12cH, 30cH or succussed 30% ethanol (control) for 21 days.
  
  
  RESULTS
  In the presence of antimycin-A, electron transport chain inhibitor, as evidenced by antimycin-A insensitive O(2) consumption, Arnica inhibited lipid peroxidation of mitochondrial membranes. In oxidative stress conditions, in the presence of Ca(2+) and inorganic phosphate, animals receiving Arnica 30cH had a significant decrease in mitochondrial O(2) consumption compared to control animals.
  
  
  CONCLUSIONS
  When administrated orally, Arnica 30cH protects against hepatic mitochondrial membrane permeabilization induced by Ca(2+) and/or Fe(2+)-citrate-mediated lipid peroxidation and fragmentation of proteins due to the attack by reactive oxygen species.
authors.affiliation: Fundação Hermínio Ometto, Uniararas, Araras, SP, Brazil.
authors.forename: Ronaldo Antônio
authors.initials: RA
authors.lastname: de Camargo
authors.forename: Ellen Dias
authors.initials: ED
authors.lastname: da Costa
authors.forename: Rosana
authors.initials: R
authors.lastname: Catisti
mesh_terms: D000284:Administration, Oral; D000818:Animals; D018650:Arnica; D002478:Cells, Cultured; D006705:Homeopathy; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species
chemical_list: D017382:Reactive Oxygen Species