title: Effect of BMP-2 from matrices of different stiffnesses for the modulation of stem cell fate.
abstract: Stem cells cultured on extracellular matrix (ECM) with different stiffnesses have been shown to engage into different lineage commitments. However, in vivo, the components of the ECM are known to bind and strongly interact with growth factors. The effect, on the stem cell fate, of the cooperation between the mechanical properties and the growth factor in the same microenvironment has not yet been investigated. Here, we propose a protocol for mimicking this stem cell microenvironment with an in vitro system. This system consists in grafting (without using a spacer) biomolecules that contain N-termini groups onto hydrogel (poly(acrylamide-co-acrylic acid)) surfaces of various stiffnesses ranging from 0.5 to 70 kPa. First, we demonstrate that the commitment of mesenchymal stem cell populations changes in response to the substrate's rigidity, with myogenic differentiation occurring at 13-17 kPa and osteogenic differentiation at 45-49 kPa. Chemical grafting of soft and stiff matrices with an osteogenic factor (BMP-2(mimetic peptide)) results only in osteogenic differentiation. Also, when grafted on even softer gels (0.5-3.5 kPa), the BMP-2(mimetic peptide) had no effect on the stem cell differentiation. We prove that correct organization of F-actin cytoskeleton due to the mechanical properties of the microenvironment is necessary for BMP-induced smad1/5/8 phosphorylation and nuclear translocation. These results suggest that stem cell differentiation is dictated mechanically, but in the presence of a biochemical factor, the effect of the mechanical factor on stem cell commitment is modified. This can explain the diversity of stem cell behaviors in vivo where different growth factors are sequestrated on the ECM.
authors.affiliation: U1026, Bioingénierie Tissulaire, Université de Bordeaux, 146 rue Léo Saignat, 33076 Bordeaux, France. omar.zouani@inserm.fr
authors.forename: Omar F
authors.initials: OF
authors.lastname: Zouani
authors.forename: Jérôme
authors.initials: J
authors.lastname: Kalisky
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Ibarboure
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Durrieu
mesh_terms: D000178:Acrylamides / Q000032:analysis / Q000737:chemistry; D000199:Actins / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism; D005109:Extracellular Matrix / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008024:Ligands; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008856:Microscopy, Fluorescence; D010012:Osteogenesis / Q000502:physiology*; D056951:Photoelectron Spectroscopy
chemical_list: D000178:Acrylamides; D000199:Actins; C525718:BMP2 protein, human; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D020100:Hydrogels; D008024:Ligands; C431184:poly(acrylamide-co-acrylic acid)