title: Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil.
abstract: In an effort to detect West Nile virus (WNV) in Brazil, we sampled serum from horses and chickens from the Pantanal region of the state of Mato Grosso and tested for flavivirus-reactive antibodies by blocking ELISA. The positive samples were further confirmed for serological evidence of WNV infection in three (8%) of the 38 horses and one (3.2%) of the 31 chickens using an 80% plaque-reduction neutralisation test (PRNT80). These results provide evidence of the circulation of WNV in chickens and horses in Pantanal.
authors.affiliation: Laboratório de Diptera, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brasil. melandri@ioc.fiocruz.br
authors.forename: Vanessa
authors.initials: V
authors.lastname: Melandri
authors.forename: Anthony Érico
authors.initials: AÉ
authors.lastname: Guimarães
authors.forename: Nicholas
authors.initials: N
authors.lastname: Komar
authors.forename: Maurício L
authors.initials: ML
authors.lastname: Nogueira
authors.forename: Adriano
authors.initials: A
authors.lastname: Mondini
authors.forename: Ana
authors.initials: A
authors.lastname: Fernandez-Sesma
authors.forename: Jeronimo
authors.initials: J
authors.lastname: Alencar
authors.forename: Irene
authors.initials: I
authors.lastname: Bosch
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001715:Bird Diseases / Q000175:diagnosis / Q000453:epidemiology*; D001938:Brazil / Q000453:epidemiology; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006734:Horse Diseases / Q000175:diagnosis / Q000453:epidemiology*; D006736:Horses; D009500:Neutralization Tests; D016036:Seroepidemiologic Studies; D014901:West Nile Fever / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D014902:West Nile virus / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral