title: The sodium-potassium pump controls the intrinsic firing of the cerebellar Purkinje neuron.
abstract: In vitro, cerebellar Purkinje cells can intrinsically fire action potentials in a repeating trimodal or bimodal pattern. The trimodal pattern consists of tonic spiking, bursting, and quiescence. The bimodal pattern consists of tonic spiking and quiescence. It is unclear how these firing patterns are generated and what determines which firing pattern is selected. We have constructed a realistic biophysical Purkinje cell model that can replicate these patterns. In this model, Na(+)/K(+) pump activity sets the Purkinje cell's operating mode. From rat cerebellar slices we present Purkinje whole cell recordings in the presence of ouabain, which irreversibly blocks the Na(+)/K(+) pump. The model can replicate these recordings. We propose that Na(+)/K(+) pump activity controls the intrinsic firing mode of cerbellar Purkinje cells.
authors.affiliation: Department of Computer Science, University of Warwick, Coventry, West Midlands, United Kingdom. mikeforrest@hotmail.com
authors.forename: Michael D
authors.initials: MD
authors.lastname: Forrest
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Wall
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Press
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Feng
mesh_terms: D000818:Animals; D003712:Dendrites / Q000187:drug effects / Q000378:metabolism; D015640:Ion Channel Gating / Q000187:drug effects; D051663:Kv1.2 Potassium Channel / Q000378:metabolism; D008297:Male; D008959:Models, Neurological; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D010042:Ouabain / Q000494:pharmacology; D011689:Purkinje Cells / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013997:Time Factors
chemical_list: D051663:Kv1.2 Potassium Channel; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase