title: Screening and identification of functional Lactobacillus specific for vegetable fermentation.
abstract: In this study, functional lactic acid bacteria (LAB) specific for vegetable fermentation were screened from Chinese sauerkraut brine by designing purpose media. The selected LAB strains showed excellent tolerance to high concentrations of bile salt and acids. They can also survive passage through the simulated gastric fluid and may be able to reach to the intestine. Through the performance measurements of vegetable fermentation, one of these strains, NCU116 was found to have the best fermentation ability, and further subjected to a series of analyses including identification of physiological and biochemical characteristics, 16S rDNA and pheS sequencing. Taken together, the strain NCU116 was identified as Lactobacillus plantarum subsp. plantarum. These results laid a good foundation for the fermentation research in the future.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. xiongtao0907@163.com
authors.forename: Tao
authors.initials: T
authors.lastname: Xiong
authors.forename: Suhua
authors.initials: S
authors.lastname: Song
authors.forename: Xuhui
authors.initials: X
authors.lastname: Huang
authors.forename: Chao
authors.initials: C
authors.lastname: Feng
authors.forename: Guangquan
authors.initials: G
authors.lastname: Liu
authors.forename: Jinqing
authors.initials: J
authors.lastname: Huang
authors.forename: Mingyong
authors.initials: M
authors.lastname: Xie
mesh_terms: D001647:Bile Acids and Salts; D005285:Fermentation; D005516:Food Microbiology; D005798:Genes, Bacterial; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014675:Vegetables / Q000382:microbiology*
chemical_list: D001647:Bile Acids and Salts; D012336:RNA, Ribosomal, 16S