title: Proteome profiling of peripheral mononuclear cells from human blood.
abstract: Peripheral blood mononuclear cells (MNCs) are accessible through blood collection and represent a useful source for investigations on disease mechanisms and treatment response. Aiming to build a reference proteome database, we generated three proteome data sets from MNCs using a combination of SDS-PAGE and nanoflow LC-MS. Experiments were performed in triplicates and 514 unique proteins were identified by at least two non-redundant peptides with 95% confidence for all replicates. Identified proteins are associated with a range of dermatologic, inflammatory and neurological conditions as well as molecular processes, such as free radical scavenging and cellular growth and proliferation. Mapping the MNC proteome provides a valuable resource for studies on disease pathogenesis and the identification of therapeutic targets.
authors.affiliation: Max Planck Institute for Psychiatry, Proteomics and Biomarkers, Munich, Germany.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Maccarrone
authors.forename: Christiane
authors.initials: C
authors.lastname: Rewerts
authors.forename: Maria
authors.initials: M
authors.lastname: Lebar
authors.forename: Christoph W
authors.initials: CW
authors.lastname: Turck
authors.forename: Daniel
authors.initials: D
authors.lastname: Martins-de-Souza
mesh_terms: D015415:Biomarkers; D001798:Blood Proteins / Q000032:analysis* / Q000737:chemistry; D030562:Databases, Protein; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry*; D010449:Peptide Mapping; D020543:Proteome / Q000032:analysis*; D040901:Proteomics
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D020543:Proteome