title: Effects of asymmetric dimethylarginine on inflammatory cytokines (TNF-α, IL-6 and IL-10) in rats.
abstract: This study is intended to examine the effects of administration of asymmetric dimethylarginine (ADMA) on the levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-10. A total of 30 Wistar adult albino rats were used. Group I was administrated (n = 10) with 1 mg/kg/day of ADMA, group II (n = 10) was administrated with 2 mg/kg/day of ADMA and the control group was administrated (n = 10) with 0.9% sodium chloride. ADMA was intraperitoneally administrated for 7 days. The serum levels of IL-6, TNF-α and IL-10 were measured. There was a significant decrease in the levels of TNF-α, IL-6 and IL-10 in group I compared with that of the control group (p < 0.001). There was also a significant decrease in the levels of IL-10 in group II compared with that of the control group (p < 0.05) but the increase was much more distinct in the levels of IL-6 and TNF-α (p < 0.001). When comparing the groups by the doses given, no difference between the levels of IL-6 and IL-10 in groups I and II (p > 0.05) was observed; the levels of TNF-α in group II were significantly lower than those of group I (p < 0.05). A significant decrease in the serum levels of inflammatory cytokines IL-6, TNF-α and IL-10, after administration of 1 mg/kg/day and 2 mg/kg/day of ADMA, indicates that ADMA has an effect on inflammation. Increase in ADMA levels in the rats shows that the effects of inflammatory cytokines were suppressed.
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey bahattinavci@hotmail.com.
authors.forename: Bahattin
authors.initials: B
authors.lastname: Avci
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey.
authors.forename: Hasan
authors.initials: H
authors.lastname: Alacam
authors.affiliation: Department of Anaesthesiology and Reanimation, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Dilek
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Kozan
mesh_terms: D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007249:Inflammation / Q000139:chemically induced*; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000097:blood; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000097:blood; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000097:blood / Q000187:drug effects
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; C018524:N,N-dimethylarginine; D001120:Arginine
keywords: ADMA; IL-10; IL-6; TNF-α; Wistar rat