title: Poly(amido amine)-based mannose-glycodendrimers as multielectron redox probes for improving lectin sensing.
abstract: An easy-to-prepare series of electroactive poly(amido amine) (PAMAM)-based dendrimers of generations G0 to G2 having mannopyranosylferrocenyl moieties in the periphery to detect carbohydrate-protein interactions is reported. The synthesis involved the functionalization of the PAMAM surface with azidomethylferrocenyl groups and subsequent coupling of mannoside units by the Cu(I)-catalyzed Huisgen reaction. The binding affinity of the series of electroactive glycodendrimers was studied by isothermal titration calorimetry (ITC) and differential pulse voltammetry (DPV). Upon complexation of the glycodendrimers conjugates with prototypical concanavalin A (Con A), voltammograms showed a decrease of the peak current. Such dendrimers showed a notable improvement of redox sensing abilities toward Con A when compared with mono- and divalent analogues, based on both the glycoside multivalent and ferrocene dendritic effects.
authors.affiliation: Department of Chemistry and Physics, University of Almería, Carretera de Sacramento s/n, 04120 Almería, Spain.
authors.forename: Manuel C
authors.initials: MC
authors.lastname: Martos-Maldonado
authors.forename: Juan M
authors.initials: JM
authors.lastname: Casas-Solvas
authors.forename: Indalecio
authors.initials: I
authors.lastname: Quesada-Soriano
authors.forename: Luis
authors.initials: L
authors.lastname: García-Fuentes
authors.forename: Antonio
authors.initials: A
authors.lastname: Vargas-Berenguel
mesh_terms: D002151:Calorimetry; D002384:Catalysis; D003208:Concanavalin A / Q000032:analysis*; D003300:Copper / Q000737:chemistry; D050091:Dendrimers / Q000737:chemistry*; D055664:Electrochemical Techniques; D004583:Electrons; D005296:Ferrous Compounds / Q000737:chemistry*; D006001:Glycoconjugates / Q000138:chemical synthesis*; D008358:Mannose / Q000737:chemistry*; D000075163:Metallocenes; D015394:Molecular Structure; D010084:Oxidation-Reduction; D011485:Protein Binding; D012680:Sensitivity and Specificity
chemical_list: D050091:Dendrimers; D005296:Ferrous Compounds; D006001:Glycoconjugates; D000075163:Metallocenes; C104700:PAMAM Starburst; D003208:Concanavalin A; D003300:Copper; D008358:Mannose; C004998:ferrocene