title: Metabolomic profilings of urine and serum from high fat-fed rats via 1H NMR spectroscopy and pattern recognition.
abstract: (1)H NMR spectroscopy in combination with multivariate statistical analysis was applied to explore the metabolic variability in urine and serum of high fat-fed rats relative to normal chow-fed ones. Metabolites contributing to intergroup discrimination identified by partial least squares discriminant analysis include 3-hydroxybutyrate, glutamate, glutamine, citrate, choline, hippurate, alanine, lactate, creatinine, taurine, acetate, etc. The aging effect along with long-term feeding was delineated with metabolic trajectory in principal component analysis score plot and age-related differences on metabolic profiling under different dietary intervention were recognised. The identified metabolites responsible for obesity were all imported into a web tool for network-based interpretation of compound lists to interpret their functional context, molecular mechanisms and disturbed signalling pathway globally and systematically. The results are useful for interpreting the pathology of obesity and further probing into the relationship between dietary-induced obesity and type 2 diabetes mellitus.
authors.affiliation: Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University, Xiamen, 361005, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Xu
authors.forename: Changqin
authors.initials: C
authors.lastname: Liu
authors.forename: Shuhui
authors.initials: S
authors.lastname: Cai
authors.forename: Jiyang
authors.initials: J
authors.lastname: Dong
authors.forename: Xuejun
authors.initials: X
authors.lastname: Li
authors.forename: Jianghua
authors.initials: J
authors.lastname: Feng
authors.forename: Zhong
authors.initials: Z
authors.lastname: Chen
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000378:metabolism; D000818:Animals; D002794:Choline / Q000378:metabolism; D019343:Citric Acid / Q000378:metabolism; D003404:Creatinine / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism; D004041:Dietary Fats / Q000009:adverse effects*; D018698:Glutamic Acid / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D006626:Hippurates / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D009765:Obesity / Q000378:metabolism; D051381:Rats; D013654:Taurine / Q000378:metabolism
chemical_list: D004041:Dietary Fats; D006626:Hippurates; D005973:Glutamine; D013654:Taurine; D019343:Citric Acid; D019344:Lactic Acid; D018698:Glutamic Acid; D003404:Creatinine; D002794:Choline; C030514:hippuric acid; D020155:3-Hydroxybutyric Acid