title: Manipulating signaling at will: chemically-inducible dimerization (CID) techniques resolve problems in cell biology.
abstract: Chemically-inducible dimerization (CID) is a powerful tool that has proved useful in solving numerous problems in cell biology and related fields. In this review, we focus on case studies where CID was able to provide insight into otherwise refractory problems. Of particular interest are the cases of lipid second messengers and small GTPases, where the "signaling paradox" (how a small pool of signaling molecules can generate a large range of responses) can be at least partly explained through results gleaned from CID experiments. We also discuss several recent technical advances that provide improved specificity in CID action, novel CID substrates that allow simultaneous orthogonal manipulation of multiple systems in one cell, and several applications that move beyond the traditional CID technique of moving a protein of interest to a specific spatiotemporal location.
authors.affiliation: Department of Cell Biology, Center for Cell Dynamics, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: DeRose
authors.forename: Takafumi
authors.initials: T
authors.lastname: Miyamoto
authors.forename: Takanari
authors.initials: T
authors.lastname: Inoue
mesh_terms: D000818:Animals; D006801:Humans; D050356:Lipid Metabolism; D020559:Monomeric GTP-Binding Proteins / Q000378:metabolism; D055503:Protein Multimerization / Q000187:drug effects*; D015398:Signal Transduction; D020123:Sirolimus / Q000494:pharmacology; D022021:Tacrolimus Binding Proteins / Q000737:chemistry / Q000187:drug effects
chemical_list: D020559:Monomeric GTP-Binding Proteins; D022021:Tacrolimus Binding Proteins; D020123:Sirolimus