title: A two-stage pretreatment approach to maximise sugar yield and enhance reactive lignin recovery from poplar wood chips.
abstract: A two-stage pretreatment approach, employing steam followed by organosolv treatment, was assessed for its ability to fractionate and recover most of the hemicellulose, lignin and cellulose components of poplar wood chips. A mild steaming stage was initially used to maximise hemicellulose sugar recovery, with 63% of the original xylan solubilised and recovered after this stage and close to 90% recovered in total. Rather than hindering subsequent organosolv delignification, the prior steam treatment enhanced lignin solubilisation with more than 66% of the original lignin removed after the two-stage pretreatment. The extracted lignin contained at least equal or greater amounts of functional groups as compared to the lignin solubilised after a single-stage organosolv pretreatment. More than 98% of the original cellulose was recovered after the two-stage pretreatment and 88% of the cellulose could be hydrolysed to glucose at enzyme loading of 5FPU/g cellulose after 72h.
authors: Forest Products Biotechnology/Bioenergy Group, Faculty of Forestry, University of British Columbia, 2424 Main Mall, Vancouver BC, Canada V6T 1Z4. Electronic address: ioannis.panagiotopoulos@ubc.ca.
authors: I A
authors: IA
authors: Panagiotopoulos
authors: R P
authors: RP
authors: Chandra
authors: J N
authors: JN
authors: Saddler
mesh_terms: D002241:Carbohydrates / Q000032:analysis; D000431:Ethanol; D006868:Hydrolysis; D008031:Lignin / Q000302:isolation & purification*; D011134:Polysaccharides / Q000302:isolation & purification*; D032107:Populus / Q000737:chemistry*; D013227:Steam; D013464:Sulfuric Acids; D014934:Wood / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D011134:Polysaccharides; D013227:Steam; D013464:Sulfuric Acids; D000431:Ethanol; C007916:hemicellulose; D008031:Lignin; C033158:sulfuric acid