title: Stat3 inhibits WTX expression through up-regulation of microRNA-370 in Wilms tumor.
abstract: Wilms tumor (WT) is a genetically heterogeneous childhood kidney tumor. Several genetic mutations have been identified in WT patients, including inactivation of WTX, somatic stabilizing CTNNB1, and p53 mutations. However, the molecular mechanisms in tumorigenesis remain largely unexplored. Stat3 is a transcription factor that can promote oncogenesis. Stat3 activation is commonly viewed as crucial for multiple tumor proliferation and metastasis. We show that Stat3 is highly activated in Wilms tumor tissues compared to those in adjacent tissues. IL-6 treatment or transfection of a constitutively activated Stat3 in G401 cells promotes cell proliferation. At the molecular level, we further elucidate that Stat3 inhibits WTX expression through up-regulation of microRNA-370. Our results suggest that Stat3/miR-370/WTX regulatory axis might be a critical mechanism in Wilms tumor cells.
authors: Department of Surgery, Childrens' Hospital Affiliated to Soochow University, Soochow University, Suzhou 215003, PR China.
authors: Xu
authors: X
authors: Cao
authors: Dehong
authors: D
authors: Liu
authors: Xiangming
authors: X
authors: Yan
authors: Ya
authors: Y
authors: Zhang
authors: Liqun
authors: L
authors: Yuan
authors: Ting
authors: T
authors: Zhang
authors: Mingcui
authors: M
authors: Fu
authors: Yun
authors: Y
authors: Zhou
authors: Jian
authors: J
authors: Wang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology; D008156:Luciferases; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D061251:Primary Cell Culture; D034741:RNA, Small Interfering / Q000235:genetics; D050796:STAT3 Transcription Factor / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection; D059016:Tumor Microenvironment / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism; D009396:Wilms Tumor / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: C517118:AMER1 protein, human; D048868:Adaptor Proteins, Signal Transducing; C508600:IL6 protein, human; D015850:Interleukin-6; C526503:MIRN370 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D025521:Tumor Suppressor Proteins; D049452:Green Fluorescent Proteins; D008156:Luciferases