title: Oxidative stress and innate immunity status in chickens exposed to high dose of ascorbic acid.
abstract: The effects of high dose ascorbic acid (10 000 mg·kg(-1) in the diet) and the transition metal on the presence of oxidative stress in the internal organs of growing chicks, as well as on the innate immune system status, were investigated. Supplementation with a high dose of ascorbic acid had pro-inflammatory effects on the intestinal mucosa, and lysozyme levels were decreased significantly in all organs studied. High-dose ascorbic acid caused an imbalance between prooxidative and antioxidative activities and was associated with the generation of semiquinone radicals. We observed that ascorbic acid increased iron and cadmium absorption. When a high dose of ascorbic acid was applied, elevated kidney and intestinal mucosa iron concentrations were observed. The amount of free malondialdehyde in the above organs has increased as well. These data have important implications for the mechanism of the oxidative stress development under the influence of high dose of ascorbic acid, indicating the importance of the side reactions of the mitochondrial electron transport chain with the formation of semiquinone radicals and the role of transition metals in this process.
authors: Laboratory of Biochemistry and Physiology of Animals, Institute of Biology, University of Latvia, Riga, Latvia.
authors: Nadezhda
authors: N
authors: Berzina
authors: Jurijs
authors: J
authors: Markovs
authors: Tatiana
authors: T
authors: Dizhbite
authors: Mirdza
authors: M
authors: Apsite
authors: Svetlana
authors: S
authors: Vasilyeva
authors: Nataliya
authors: N
authors: Basova
authors: Galina
authors: G
authors: Smirnova
authors: Sergejs
authors: S
authors: Isajevs
mesh_terms: D000327:Adsorption; D000825:Animal Structures / Q000187:drug effects / Q000502:physiology; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D016227:Benzoquinones / Q000378:metabolism; D002104:Cadmium / Q000378:metabolism; D002645:Chickens / Q000502:physiology*; D004578:Electron Spin Resonance Spectroscopy; D007113:Immunity, Innate / Q000187:drug effects*; D007501:Iron / Q000378:metabolism; D009113:Muramidase / Q000378:metabolism; D009928:Organ Specificity; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016227:Benzoquinones; D017382:Reactive Oxygen Species; D002104:Cadmium; C025232:semiquinone radicals; D007501:Iron; D009113:Muramidase; D001205:Ascorbic Acid
keywords: ascorbic acid; cadmium; chicken; immune system; iron; oxidative stress