title: Target-responsive "sweet" hydrogel with glucometer readout for portable and quantitative detection of non-glucose targets.
abstract: Portable devices with the advantages of rapid, on-site, user-friendly, and cost-effective assessment are widely applied in daily life. However, only a limited number of quantitative portable devices are commercially available, among which the personal glucose meter (PGM) is the most successful example and has been the most widely used. However, PGMs can detect only blood glucose as the unique target. Here we describe a novel design that combines a glucoamylase-trapped aptamer-cross-linked hydrogel with a PGM for portable and quantitative detection of non-glucose targets. Upon target introduction, the hydrogel collapses to release glucoamylase, which catalyzes the hydrolysis of amylose to produce a large amount of glucose for quantitative readout by the PGM. With the advantages of low cost, rapidity, portability, and ease of use, the method reported here has the potential to be used by the public for portable and quantitative detection of a wide range of non-glucose targets.
authors: State Key Laboratory of Physical Chemistry of Solid Surfaces, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors: Ling
authors: L
authors: Yan
authors: Zhi
authors: Z
authors: Zhu
authors: Yuan
authors: Y
authors: Zou
authors: Yishun
authors: Y
authors: Huang
authors: Dewen
authors: D
authors: Liu
authors: Shasha
authors: S
authors: Jia
authors: Dunming
authors: D
authors: Xu
authors: Min
authors: M
authors: Wu
authors: Yu
authors: Y
authors: Zhou
authors: Shuang
authors: S
authors: Zhou
authors: Chaoyong James
authors: CJ
authors: Yang
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis* / Q000378:metabolism; D000688:Amylose / Q000737:chemistry / Q000378:metabolism; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D055162:Biocatalysis; D015190:Blood Glucose Self-Monitoring; D003042:Cocaine / Q000032:analysis* / Q000378:metabolism; D005947:Glucose / Q000032:analysis / Q000096:biosynthesis; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry* / Q000378:metabolism; D006868:Hydrolysis; D000520:alpha-Glucosidases / Q000737:chemistry* / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D000255:Adenosine Triphosphate; D000688:Amylose; D000520:alpha-Glucosidases; D003042:Cocaine; D005947:Glucose