title: Cheese surface microbiota complexity: RT-PCR-DGGE, a tool for a detailed picture?
abstract: In this work, a culture-independent approach, based on PCR-DGGE and RT-PCR-DGGE, has been used to study the succession of bacterial communities that are encountered in Fontina PDO cheese. As already found for other smear ripened cheeses, it appeared that coryneform bacteria were actively present and could therefore be considered determinant in rind formation. DGGE profiles, especially at the RNA level, have shown the presence of Brevibacterium, Corynebacterium and Arthrobacter genera. RT-PCR-DGGE gels have lead to a richer band profile than the one obtained on the basis of DNA analysis, thus indicating that RNA analysis can highlight bacterial species that DNA analysis is not able to show. Thus, the biodiversity of the Fontina PDO surface has been described better by means of RT-PCR-DGGE, and RNA molecules should be considered a more informative target than DNA.
authors: DIVAPRA, University of Turin, Grugliasco, Italy. paola.dolci@unito.it
authors: Paola
authors: P
authors: Dolci
authors: Simona
authors: S
authors: Zenato
authors: Rita
authors: R
authors: Pramotton
authors: Andrea
authors: A
authors: Barmaz
authors: Valentina
authors: V
authors: Alessandria
authors: Kalliopi
authors: K
authors: Rantsiou
authors: Luca
authors: L
authors: Cocolin
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics; D044822:Biodiversity; D002611:Cheese / Q000382:microbiology*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D005516:Food Microbiology; D054892:Metagenome / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial