title: Effect of the environmental pollutant bisphenol A dimethacylate (BAD) on Ca2+ movement and viability in OC2 human oral cancer cells.
abstract: The environmental pollutant bisphenol A dimethacylate (BAD) has been used as a dental composite. The effect of BAD on cytosolic Ca(2+) concentrations ([Ca(2+)]i) and viability in OC2 human oral cancer cells was explored. The Ca(2+)-sensitive fluorescent dye fura-2 was applied to measure [Ca(2+)]i. BAD induced [Ca(2+)]i rises in a concentration-dependent manner. The response was reduced by removing extracellular Ca(2+). BAD-evoked Ca(2+) entry was suppressed by nifedipine, econazole, and SK&F96365. In Ca(2+)-free medium, incubation with the endoplasmic reticulum Ca(2+) pump inhibitor thapsigargin abolished BAD-induced [Ca(2+)]i rise. Inhibition of phospholipase C with U73122 did not alter BAD-induced [Ca(2+)]i rise. At 10-30μM, BAD inhibited cell viability, which was not reversed by chelating cytosolic Ca(2+). BAD (20-30μM) also induced apoptosis. Collectively, in OC2 cells, BAD induced a [Ca(2+)]i rise by evoking phospholipase C-independent Ca(2+) release from the endoplasmic reticulum and Ca(2+) entry via store-operated Ca(2+) channels. BAD also caused apoptosis.
authors: Department of Pediatrics, Ping Tung Christian Hospital, Ping Tung 900, Taiwan.
authors: Jau-Min
authors: JM
authors: Chien
authors: Chiang-Ting
authors: CT
authors: Chou
authors: Yi-Chau
authors: YC
authors: Lu
authors: Ti
authors: T
authors: Lu
authors: Chao-Chuan
authors: CC
authors: Chi
authors: Li-Ling
authors: LL
authors: Tseng
authors: Shiuh-Inn
authors: SI
authors: Liu
authors: Jin-Shiung
authors: JS
authors: Cheng
authors: Chun-Chi
authors: CC
authors: Kuo
authors: Wei-Zhe
authors: WZ
authors: Liang
authors: Chung-Ren
authors: CR
authors: Jan
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001559:Benzhydryl Compounds / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism; D004785:Environmental Pollutants / Q000494:pharmacology; D006801:Humans; D008689:Methacrylates / Q000494:pharmacology*; D009062:Mouth Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology*; D010738:Type C Phospholipases / Q000378:metabolism; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D002118:Calcium; D004785:Environmental Pollutants; D008689:Methacrylates; D010738:Type C Phospholipases; C002462:4,4'-isopropylidenediphenol dimethacrylate; D000098826:Bisphenol A Compounds