title: A rapid and sensitive LC-MS/MS method for the determination of osthole in rat plasma: application to pharmacokinetic study.
abstract: Osthole, a major component isolated from the fruit of Cnidium monnieri (L.) Cusson, has been widely used in traditional Chinese medicine. We developed and validated a rapid and sensitive LC-MS/MS method for the quantification of osthole in rat plasma. Sample preparation involved simple liquid-liquid extraction by ethyl acetate after addition of imperatorin as internal standard (IS). The analyte was separated using a C(18) column with the mobile phase of methanol-0.1% formic acid (80:20, v/v) at a flow rate of 0.4 mL/min. The elutes were detected under positive electrospray ionization in multiple reaction monitoring mode. The method was sensitive with 0.5 ng/mL as the lower limit of detection. Good linearity was obtained over the range of 1.0-500.0 ng/mL. The intra and inter-batch accuracy for osthole in rat plasma samples ranged from 99.5 to 108.1% and the variation was <8.9%. The stability, extraction efficiency and matrix effect were also acceptable. This method was successfully applied to the pharmacokinetic study of osthole in rat after intravenous and oral administration.
authors: School of Pharmacy, Nanjing University of Chinese Medicine, 138 Xianlin Street, Nanjing 210046, China.
authors: Fei
authors: F
authors: Yun
authors: An
authors: A
authors: Kang
authors: Jinjun
authors: J
authors: Shan
authors: Xiaoli
authors: X
authors: Zhao
authors: Xiaolin
authors: X
authors: Bi
authors: Liuqing
authors: L
authors: Di
mesh_terms: D061605:Administration, Intravenous; D000284:Administration, Oral; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D003374:Coumarins / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D004355:Drug Stability; D011564:Furocoumarins; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D003374:Coumarins; D011564:Furocoumarins; C031534:imperatorin; C046627:osthol