title: Scaffold effects on osteogenic differentiation of equine mesenchymal stem cells: an in vitro comparative study.
abstract: The in vitro viability, osteogenic differentiation, and mineralization of four different equine mesenchymal stem cells (MSCs) from bone marrow, periosteum, muscle, and adipose tissue are compared, when they are cultured with different collagen-based scaffolds or with fibrin glue. The results indicate that bone marrow cells are the best source of MSCs for osteogenic differentiation, and that an electrochemically aggregated collagen gives the highest cell viability and best osteogenic differentiation among the four kinds of scaffolds studied.
authors: Comparative Orthopaedic Research Laboratory, Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, 550 University Av. Charlottetown, Prince Edward Island, C1A4P3, Canada. rodninof@gmail.com
authors: Rodolfo
authors: R
authors: Nino-Fong
authors: Laurie A
authors: LA
authors: McDuffee
authors: Blanca P
authors: BP
authors: Esparza Gonzalez
authors: M Ramesh
authors: MR
authors: Kumar
authors: Erika F
authors: EF
authors: Merschrod S
authors: Kristin M
authors: KM
authors: Poduska
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology*; D015718:Fibrin Tissue Adhesive / Q000737:chemistry / Q000494:pharmacology*; D006736:Horses; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D018482:Muscle, Skeletal / Q000166:cytology; D010011:Osteocytes / Q000166:cytology / Q000187:drug effects*; D010521:Periosteum / Q000166:cytology; D020033:Protein Isoforms / Q000737:chemistry / Q000494:pharmacology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D015718:Fibrin Tissue Adhesive; D020033:Protein Isoforms; D003094:Collagen