title: In vivo examination of the local inflammatory response after implantation of Ti6Al4V samples with a combined low-temperature plasma treatment using pulsed magnetron sputtering of copper and plasma-polymerized ethylenediamine.
abstract: Copper (Cu) could serve as antibacterial coating for Ti6Al4V implants. An additional cell-adhesive layer might compensate Cu cytotoxicity. This study aimed at in vitro and in vivo evaluation of low-temperature plasma treatment of Ti6Al4V plates with Ti/Cu magnetron sputtering (Ti6Al4V-Ti/Cu), plasma-polymerized ethylenediamine (Ti6Al4V-PPEDA), or both (Ti6Al4V-Ti/Cu-PPEDA). Ti6Al4V-Ti/Cu and Ti6Al4V-Ti/Cu-PPEDA had comparable in vitro Cu release and antibacterial effectiveness. Following intramuscular implantation of Ti6Al4V-Ti/Cu, Ti6Al4V-PPEDA, Ti6Al4V-Ti/Cu-PPEDA and Ti6Al4V controls for 7, 14 and 56 days with 8 rats/day, peri-implant tissue was immunohistochemically examined for different inflammatory cells. Ti6Al4V-PPEDA had more mast cells and NK cells than Ti6Al4V, and more tissue macrophages, T lymphocytes, mast cells and NK cells than Ti6Al4V-Ti/Cu-PPEDA. Ti6Al4V-Ti/Cu had more mast cells than Ti6Al4V and Ti6Al4V-Ti/Cu-PPEDA. Results indicate that PPEDA-mediated cell adhesion counteracted Cu cytotoxicity. Ti6Al4V-Ti/Cu-PPEDA differed from Ti6Al4V only for mast cells on day 56. Altogether, implants with both plasma treatments had antibacterial properties and did not increase inflammatory reactions.
authors: Department of Surgery, University of Greifswald, Greifswald, Germany.
authors: Andreas
authors: A
authors: Hoene
authors: Maciej
authors: M
authors: Patrzyk
authors: Uwe
authors: U
authors: Walschus
authors: Vítězslav
authors: V
authors: Straňák
authors: Rainer
authors: R
authors: Hippler
authors: Holger
authors: H
authors: Testrich
authors: Jürgen
authors: J
authors: Meichsner
authors: Birgit
authors: B
authors: Finke
authors: Henrike
authors: H
authors: Rebl
authors: Barbara
authors: B
authors: Nebe
authors: Carmen
authors: C
authors: Zietz
authors: Rainer
authors: R
authors: Bader
authors: Andreas
authors: A
authors: Podbielski
authors: Michael
authors: M
authors: Schlosser
mesh_terms: D000497:Alloys; D000818:Animals; D018441:Biofilms; D003300:Copper / Q000737:chemistry*; D005029:Ethylenediamines / Q000737:chemistry*; D007150:Immunohistochemistry; D007249:Inflammation / Q000209:etiology* / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D008826:Microbial Sensitivity Tests; D058626:Plasma Gases; D051381:Rats; D011917:Rats, Inbred Lew; D013211:Staphylococcus aureus; D013696:Temperature; D014025:Titanium / Q000009:adverse effects*
chemical_list: D000497:Alloys; D005029:Ethylenediamines; D058626:Plasma Gases; C031462:titanium alloy (TiAl6V4); C031234:ethylenediamine; D003300:Copper; D014025:Titanium