title: Changes in the level of spontaneous DNA damage in whole blood leukocytes during storage.
abstract: We evaluated structural damage to DNA (%TDNA) in blood leukocytes from healthy donors of different age at different periods (0-6 days) of blood storage at 4-8°C. It was found that the basal level of DNA damage increased and intracellular antioxidant level decreased during storage. Mean %TDNA was 6.8±3.3% in the fresh blood and 19.2±8.1% after 5-day storage. The experiments with exposure to reactive oxygen species induced by irradiation suggest that depletion of low-molecular-weight endogenous antioxidants occurs as soon as after 5-h storage. Our results suggest that storage time should be taken into account when assessing the basal and induced levels of leukocyte DNA damage by the comet assay.
authors: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, Russia. sirota@iteb.ru
authors: N P
authors: NP
authors: Sirota
authors: E A
authors: EA
authors: Kuznetsova
mesh_terms: D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000032:analysis; D001800:Blood Specimen Collection; D004247:DNA / Q000097:blood*; D004249:DNA Damage; D006801:Humans; D007962:Leukocytes / Q000737:chemistry*; D008875:Middle Aged; D017382:Reactive Oxygen Species; D012034:Refrigeration; D013997:Time Factors; D055815:Young Adult
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D004247:DNA