title: Lack of micronuclei induction by fumonisin B(1) mycotoxin in BALB/c mice.
abstract: The genotoxic potential of fumonisin B(1) (FB(1)) in vivo in BALB/c mice (male and female) was assessed by induction of micronuclei (MN) formation in bone marrow polychromatic erythrocytes. The ratio of polychromatic erythrocytes to normochromatic erythrocytes (PCE/NCE) was also determined. Mice were injected intraperitoneally (i.p.) with FB(1) at a low dose (0.1 mg kg(-1) body mass), middle dose (1.0 mg kg(-1) body mass) and high dose (10 mg kg(-1) body mass) as single and multiple doses in normal saline to test the genotoxicity. Mitomycin C, a known clastogen, was used as positive control. The frequency of MN and the PCE/NCE value in animals treated with FB(1) at low, middle, and high doses in single dose studies, and the frequency of MN in multiple dose studies, were statistically non-significant from that of the controls injected with saline only. The multiple dose studies at all doses revealed that the PCE/NCE value was found to be reduced upon exposure to FB(1) as compared to the controls. In animals injected with multiple low doses of FB(1), the PCE/NCE value was found to be 0.66 in males and 0.82 in the females; at multiple middle doses the value was 0.30 in males and 0.41 in the females and was statistically significant (p < 0.001); however, at multiple high doses, the ratio was found to be 0.36 in both males and females. The present study confirms that FB(1) is non-genotoxic in nature while the reduced ratio of PCE/NCE suggests the cytotoxic nature of FB(1).
authors: Department of Biochemistry, University College of Science, Osmania University, Hyderabad 500 007, Andhra Pradesh, India.
authors: Rupula
authors: R
authors: Karuna
authors: B Sashidhar
authors: BS
authors: Rao
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects*; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects*; D005260:Female; D037341:Fumonisins / Q000633:toxicity*; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008853:Microscopy; D009153:Mutagens / Q000633:toxicity*; D013194:Staining and Labeling
chemical_list: D037341:Fumonisins; D009153:Mutagens; C056933:fumonisin B1