title: Whipple disease diagnosed with PCR using formalin-fixed paraffin-embedded specimens of the intestinal mucosa.
abstract: We herein present the case of a 54-year-old Japanese woman with Whipple disease diagnosed with polymerase chain reaction (PCR) using formalin-fixed paraffin-embedded (FFPE) specimens. The patient complained of weight loss, diarrhea and arthralgia. An endoscopic examination revealed swollen villi in the duodenum and ileum. Pathology demonstrated the presence of numerous macrophages filled with diastase-resistant PAS-positive particles. PCR using FFPE specimens amplified a fragment of 16S rDNA from Tropheryma whipplei. After the administration of ceftriaxone followed by trimethoprim/sulfamethoxazole, no signs of recurrence were observed for two years. The use of FFPE specimens for PCR should be considered for the prompt diagnosis and prevention of disease progression.
authors: Department of Pathology and Molecular Medicine, Hirosaki University Graduate School of Medicine, Japan.
authors: Nobuhisa
authors: N
authors: Yajima
authors: Ryuichi
authors: R
authors: Wada
authors: Sotai
authors: S
authors: Kimura
authors: Yutaka
authors: Y
authors: Matsuzaki
authors: Daisuke
authors: D
authors: Chiba
authors: Yoshihito
authors: Y
authors: Ebina
authors: Kiyofumi
authors: K
authors: Ohkusu
authors: Soroku
authors: S
authors: Yagihashi
mesh_terms: D001483:Base Sequence; D005260:Female; D005557:Formaldehyde; D006801:Humans; D007413:Intestinal Mucosa / Q000473:pathology*; D008875:Middle Aged; D008969:Molecular Sequence Data; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction / Q000379:methods*; D008061:Whipple Disease / Q000175:diagnosis* / Q000235:genetics*
chemical_list: D005557:Formaldehyde