title: Effect of PTEN antisense oligonucleotide on oesophageal squamous cell carcinoma cell lines.
abstract: OBJECTIVE
  To investigate the effects of a phosphatase and tensin homologue (PTEN) antisense oligonucleotide on PTEN and mammalian target of rapamycin (mTOR) mRNA and protein, cell proliferation and apoptosis in oesophageal squamous cell carcinoma (OCSS) cell lines.
  
  
  METHODS
  EC9706 and EC1 cells were transfected with PTEN antisense oligonucleotide, sense oligonucleotide or nonsense oligonucleotide. Cell proliferation and apoptosis were quantified. Immuno cyto chemistry and in situ hybridization were used to determine PTEN and mTOR protein  and mRNA levels, respectively.
  
  
  RESULTS
  Transfection with PTEN antisense oligonucleotide dose- and time-dependently enhanced cell proliferation and inhibited apoptosis in both EC9706 and EC1 cells. PTEN mRNA and protein were significantly downregulated, and mTOR protein and mRNA were  significantly upregulated.
  
  
  CONCLUSIONS
  These data suggest that PTEN is an important tumour suppressor gene in the development of OSCC.
authors: Department of Oncology, First Affiliated Hospital, Zhengzhou University, Zhengzhou, China.
authors: M M
authors: MM
authors: Sun
authors: M Z
authors: MZ
authors: Zhang
authors: Y
authors: Y
authors: Chen
authors: S L
authors: SL
authors: Li
authors: W
authors: W
authors: Zhang
authors: G W
authors: GW
authors: Ya
authors: K S
authors: KS
authors: Chen
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism*; D000077277:Esophageal Squamous Cell Carcinoma; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D051059:PTEN Phosphohydrolase / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D058570:TOR Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection; D015854:Up-Regulation
chemical_list: D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human