title: Seeds of Peganum Harmala L. chemical analysis, antimalarial and antioxidant activities, and cytotoxicity against human breast cancer cells.
abstract: The present study evaluated the levels of total phenolics, flavonoids, tannins and anthocyanins from Peganum harmala L. seeds and determined their antioxidant, antiplasmodial and anticancer potentials. Antioxidant activity was determined by DPPH and ABTS assays. Extracts of P. harmala seeds from Oudref and Djerba (two places in Tunisia) were obtained by successive extraction solvents: petroleum ether, chloroform, ethyl acetate, ethanol and water. Their composition was evaluated for phenolics (gallic acid equivalent 2.48 to 72.52 g/kg), tannins (catechin equivalent 0 to 25.27 g/kg), anthocyanins (cyanidin equivalent 0 to 20.56 mg/kg) and flavonoids (quercetin equivalent 0 to 3.12 g/kg). Ethanolic extract exerted the highest activities against a chloroquine-resistant strain of Plasmodium falciparum (IC₅₀=23 mg/L), against human breast cancer cells MCF7 (IC₅₀=32 mg/L) and against free radical (IC₅₀=19.09±3.07 mg/L). Correlations were studied between each chemical family and the three activities. Total phenolics content exhibited the highest correlation with antiplasmodial activity (R²=0.92) and with anticancer activity (R²=0.86), respectively.
authors: Naziha
authors: N
authors: Chabir
authors: Hany
authors: H
authors: Ibrahim
authors: Hany
authors: H
authors: Romdhane
authors: Alexis
authors: A
authors: Valentin
authors: Beatrice
authors: B
authors: Moukarzel
authors: Mohamed
authors: M
authors: Mars
authors: Jalloul
authors: J
authors: Bouajila
mesh_terms: D000872:Anthocyanins / Q000302:isolation & purification; D000962:Antimalarials / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D052160:Benzothiazoles / Q000037:antagonists & inhibitors; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D002470:Cell Survival / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects / Q000469:parasitology; D005260:Female; D005419:Flavonoids / Q000302:isolation & purification; D006801:Humans; D059625:Liquid-Liquid Extraction; D061986:MCF-7 Cells; D021261:Parasitic Sensitivity Tests; D032443:Peganum / Q000737:chemistry*; D010636:Phenols / Q000302:isolation & purification; D010851:Picrates / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000737:chemistry; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000254:growth & development; D012639:Seeds / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013451:Sulfonic Acids / Q000037:antagonists & inhibitors; D013634:Tannins / Q000302:isolation & purification
chemical_list: D000872:Anthocyanins; D000962:Antimalarials; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D005419:Flavonoids; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D013451:Sulfonic Acids; D013634:Tannins; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl