title: Frequency and determinants of pregnancy-induced child-specific sensitization.
abstract: The aim of this study was to define the frequency and determinants of pregnancy-induced child-specific sensitization shortly after full-term delivery using sensitive single HLA-antigen beads (SAB) and high resolution HLA-typing of the mothers and their children (n = 301). A positive SAB result was defined by a background normalized ratio >1 or a mean fluorescence intensity (MFI) >300, >500 and >1000, respectively. The overall frequency of pregnancy-induced sensitization determined by SAB shortly after full-term delivery was between 45% (MFI > 1000 cut-off) and 76% (ratio cut-off). The rate of child-specific sensitization at the HLA-A/B/C/DRB1 loci was between 28% (MFI > 1000 cut-off) and 38% (ratio cut-off). The number of live birth was associated with a higher frequency of sensitization, which was driven by child-specific, but not third party HLA-antibodies. There was a clear hierarchy of sensitization among the investigated loci (B-locus: 31%; A-locus: 26%; DRB1-locus: 20%; C-locus: 15%; p < 0.0001). Some mismatched paternal HLA-antigens led to a significantly higher rate of sensitization than the average (e.g. HLA-A2, HLA-B49, HLA-B51, HLA-C*15). Furthermore, the mother's own HLA-phenotype--especially HLA-A/B homozygosity--was associated with a higher rate and broadness of sensitization. The number of mismatched HLA-A/B/C eplets strongly correlated with the rate of child-specific class I sensitization.
authors: Immunobiology, University Hospital Basel, Switzerland.
authors: G
authors: G
authors: Hönger
authors: I
authors: I
authors: Fornaro
authors: C
authors: C
authors: Granado
authors: J-M
authors: JM
authors: Tiercy
authors: I
authors: I
authors: Hösli
authors: S
authors: S
authors: Schaub
mesh_terms: D000328:Adult; D000483:Alleles; D002648:Child; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006680:HLA Antigens / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D006967:Hypersensitivity / Q000209:etiology*; D011247:Pregnancy; D011248:Pregnancy Complications / Q000209:etiology*; D011379:Prognosis
chemical_list: D006680:HLA Antigens