title: [Expression of neuropilin 2 in human colorectal carcinoma and its correlation with lymphangiogenesis and lymphatic metastasis of tumor].
abstract: OBJECTIVE
  To explore the relationship between neuropilin 2 (NRP-2) and lymphangiogenesis and lymphatic metastasis of human colorectal carcinoma (CRC), as well as the expression of NRP-2 in CRC tissues.
  
  
  METHODS
  A total of 55 cases of CRC, adjacent and normal tissues of surgical resection were randomly selected at our hospital from March 2010 to January 2012. All pathological findings were confirmed by histopathology. The expression of NRP-2 was detected with reverse transcription (RT)-PCR and immunohistochemistry in parenchymatous and surrounding malignant tissues. Then lymphangiogenesis was marked with D2-40 monoclonal antibody and microlymphatic density (MLD) counted.
  
  
  RESULTS
  Significant differences of MLD existed between those of tumor region (39 ± 19) and tumor margin (53 ± 26, P < 0.01). Both the number and shape of lymphangiogenesis were different between the parenchymatous and surrounding tissues. The expression of NRP-2 had a positive correlation with MLD both at the protein level (r = 0.325, P < 0.05) and at the gene level (r = 0.545, P = 0.000). And it was also correlated with the differentiation degree, infiltrative degree, lymphovascular invasion, lymph node metastasis and Dukes tumor staging (all P < 0.05).
  
  
  CONCLUSIONS
  The expression of NRP-2 may regulate lymphangiogenesis and it may play an important role in the incidence and development of CRC.
authors: Department of Oncological Surgery, Hebei Union University Affiliated Hospital, Tangshan 063000, China. xwg853@163.com
authors: Wei-guo
authors: WG
authors: Xu
authors: Yu-peng
authors: YP
authors: Wang
authors: Zhi-yi
authors: ZY
authors: Guo
authors: Yuan-shui
authors: YS
authors: Chen
authors: Hong-chao
authors: HC
authors: Dong
authors: Peng-dong
authors: PD
authors: Zhang
authors: Yun
authors: Y
authors: Liu
authors: Yue-peng
authors: YP
authors: Zhao
authors: Hong
authors: H
authors: Sun
authors: He-liang
authors: HL
authors: Liu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D042583:Lymphangiogenesis; D008207:Lymphatic Metastasis; D042601:Lymphatic Vessels / Q000473:pathology; D008297:Male; D055806:Microvessels / Q000473:pathology*; D008875:Middle Aged; D009367:Neoplasm Staging; D039943:Neuropilin-2 / Q000378:metabolism*
chemical_list: D039943:Neuropilin-2; C581308:neuropilin-2, human