title: Calreticulin expression in infiltrating ductal breast carcinomas: relationships with disease progression and humoral immune responses.
abstract: The aim of this study was to evaluate calreticulin expression in infiltrating ductal breast carcinomas (IDCAs), as well as its relationships with clinicopathological parameters of the disease. Using a two-dimensional gel electrophoresis/matrix-assisted laser desorption ionization time of flight mass spectrometry investigation coupled to an immunohistochemical approach, we have assessed the expression of calreticulin in IDCAs, as well as in other types of breast tumors. The humoral immune response against calreticulin was estimated using a serological proteomics-based strategy. Proteomic analyses revealed an increased expression of calreticulin in IDCA tumors. Using immunohistochemistry, overexpression of calreticulin was confirmed in 51 additional tumor specimens. Statistical analyses revealed, however, no significant correlations between calreticulin expression and clinicopathological parameters of the disease including tumor stage, patient age, SBR grade, and lymph node metastasis occurrence. A significant association was found, however, with estrogen receptor status. This study demonstrates the upregulation of calreticulin in IDCA tissues which may highlight its involvement in breast cancer development. Our findings also support a link between calreticulin expression and estrogen transduction pathways. Our results do not, however, support the involvement of calreticulin in the development of a humoral immune response in IDCAs.
authors: Laboratoire d'Immuno-Oncologie Moléculaire, Faculté de Médecine de Monastir, Monastir, Tunisia.
authors: Maria
authors: M
authors: Kabbage
authors: Mounir
authors: M
authors: Trimeche
authors: Sarra
authors: S
authors: Bergaoui
authors: Philippe
authors: P
authors: Hammann
authors: Lauriane
authors: L
authors: Kuhn
authors: Bechr
authors: B
authors: Hamrita
authors: Hela
authors: H
authors: ben Nasr
authors: Anouar
authors: A
authors: Chaieb
authors: Lotfi
authors: L
authors: Chouchane
authors: Karim
authors: K
authors: Chahed
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001943:Breast Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D037282:Calreticulin / Q000378:metabolism*; D018270:Carcinoma, Ductal, Breast / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D018450:Disease Progression; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D056724:Immunity, Humoral / Q000276:immunology*; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor; D037282:Calreticulin