title: Identification of osteoarthritis biomarkers by proteomic analysis of synovial fluid.
abstract: OBJECTIVE
  To use proteomic analysis to identify novel candidate biomarker proteins in synovial fluid for the differential diagnosis of osteoarthritis and rheumatoid arthritis.
  
  
  METHODS
  Synovial fluid samples were analysed using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). Data were used to generate an artificial neural network (ANN). The identification of one protein peak was confirmed via Western blotting.
  
  
  RESULTS
  Fluid samples were analysed from 36 patients with osteoarthritis and 24 with rheumatoid arthritis. In total, three protein peaks (mass-to-charge ratio [m/z] 3893, 10,576 and 14,175 Da) were identified as potential biomarkers for osteoarthritis. The ANN differentiated between osteoarthritis and rheumatoid arthritis with a sensitivity of 89.4% and a specificity of 91.2%. The protein peak at m/z 10 576 was identified as S100 calcium binding protein A12 (S100A12).
  
  
  CONCLUSIONS
  A combination of SELDI-TOF-MS and ANN identified osteoarthritis biomarkers. SELDI-TOF-MS may be a useful tool in the screening of synovial fluid for osteoarthritis diagnosis.
authors: Cancer Therapy and Research Centre, Shandong Provincial Hospital, Shandong University, Jinan Province, China.
authors: M Y
authors: MY
authors: Han
authors: J J
authors: JJ
authors: Dai
authors: Y
authors: Y
authors: Zhang
authors: Q
authors: Q
authors: Lin
authors: M
authors: M
authors: Jiang
authors: X Y
authors: XY
authors: Xu
authors: Q
authors: Q
authors: Liu
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001172:Arthritis, Rheumatoid / Q000175:diagnosis* / Q000378:metabolism*; D015415:Biomarkers / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016571:Neural Networks, Computer; D010003:Osteoarthritis / Q000175:diagnosis* / Q000378:metabolism*; D040081:Protein Array Analysis; D020543:Proteome / Q000032:analysis; D040901:Proteomics; D009418:S100 Proteins / Q000032:analysis; D000067616:S100A12 Protein; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013582:Synovial Fluid / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D020543:Proteome; D009418:S100 Proteins; D000067616:S100A12 Protein; C090441:S100A12 protein, human