title: Moderate expression of Wnt signaling genes is essential for porcine parthenogenetic embryo development.
abstract: Parthenogenetic embryos are invariably lost in mid-gestation, possibly due to the lack of the paternal genome and the consequent induction of aberrant gene expression. Wnt signaling is essential for embryonic development; however, the studies of this pathway in porcine parthenogenetic embryos have been limited. Here, the role of Wnt signaling in porcine parthenogenetic embryos was studied. In vivo embryos were used as controls. Single cell quantitative real-time PCR showed that Wnt signaling was down-regulated in porcine parthenogenetic embryos. Furthermore, immunofluorescence staining and real-time PCR demonstrated that porcine parthenogenetic embryo development was largely unaffected by the inhibition of Wnt signaling with IWP-2, but blastocyst hatching and trophectoderm development was blocked. In addition, parthenogenetic blastocyst hatching was improved by the activation of Wnt signaling by BIO. However, the developmental competency of porcine embryos, including blastocyst hatching, was impaired and apoptosis was induced upon the excessive activation of Wnt signaling. These findings constitute novel evidence that Wnt signaling is important for porcine pre-implantation development and that its down-regulation may lead to the low hatching rate of porcine parthenogenetic blastocysts.
authors: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Science, Jilin University, Changchun 130062, China.
authors: Yongye
authors: Y
authors: Huang
authors: Hongsheng
authors: H
authors: Ouyang
authors: Wanhua
authors: W
authors: Xie
authors: Xianju
authors: X
authors: Chen
authors: Chaogang
authors: C
authors: Yao
authors: Yang
authors: Y
authors: Han
authors: Xiaolei
authors: X
authors: Han
authors: Qi
authors: Q
authors: Song
authors: Daxin
authors: D
authors: Pang
authors: Xiaochun
authors: X
authors: Tang
mesh_terms: D000081:Acetamides / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015536:Down-Regulation; D046149:Embryo Culture Techniques; D047108:Embryonic Development / Q000187:drug effects; D005260:Female; D018507:Gene Expression Regulation, Developmental; D007211:Indoles / Q000494:pharmacology; D010091:Oximes / Q000494:pharmacology; D010312:Parthenogenesis; D011247:Pregnancy; D013552:Swine; D051153:Wnt Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D060449:Wnt Signaling Pathway / Q000187:drug effects
chemical_list: C483321:6-bromoindirubin-3'-oxime; D000081:Acetamides; D007211:Indoles; D010091:Oximes; D051153:Wnt Proteins; C030686:acetamide