title: In vitro digestion with proteases producing MHC class II ligands.
abstract: Proteases generate peptides that bind to MHC class II molecules to interact with a wide diversity of CD4(+) T cells. They are expressed in dedicated organelles: endosomes and lysosomes of professional antigen presenting cells (pAPCs) such as B cells, macrophages, and dendritic cells. The identification of endosomal proteases which produce antigenic peptides is important, for example, for better vaccination and to prevent autoimmune diseases. Here, we describe a panel of technics (in vitro digestion assays of protein with recombinant proteases or purified endosomes/lysosomes, T cell stimulation) to monitor the production of MHC class II ligands.
authors: Sorbonne Paris Cité, Faculté de médecine René Descartes, INSERM U1013 and Université Paris Descartes, Paris, France.
authors: Mira
authors: M
authors: Tohmé
authors: Sorbonne Paris Cité, Faculté de médecine René Descartes, INSERM U1013 and Université Paris Descartes, Paris, France.
authors: Sophia
authors: S
authors: Maschalidi
authors: Sorbonne Paris Cité, Faculté de médecine René Descartes, INSERM U1013 and Université Paris Descartes, Paris, France. benedicte.manoury@inserm.fr.
authors: Bénédicte
authors: B
authors: Manoury
mesh_terms: D000938:Antigen-Presenting Cells / Q000166:cytology; D002458:Cell Fractionation; D011992:Endosomes / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D008024:Ligands; D008247:Lysosomes / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D059748:Proteolysis; D013601:T-Lymphocytes / Q000166:cytology
chemical_list: D000949:Histocompatibility Antigens Class II; D008024:Ligands; D010447:Peptide Hydrolases