title: T helper 17 cells are involved in the local and systemic inflammatory response in community-acquired pneumonia.
abstract: BACKGROUND
  Recent findings in mouse models suggest that T helper (Th)17 cells, characterised by production of interleukin (IL)-17A and IL-22, are involved in the immunopathogenesis of pneumonia.
  
  
  OBJECTIVE
  In this study, we aimed to identify the involvement of Th17 cells in human community-acquired pneumonia (CAP).
  
  
  METHODS
  Within 24 h of admission, T cells from peripheral blood (n=39) and bronchoalveolar lavage (BAL, n=20) of CAP patients and of 10 healthy individuals were analysed by intracellular flow cytometry for the production of various cytokines, including IL-17A and IL-22. Peripheral blood T cells were also analysed 7 and 30 days after admission. Th17 cytokine profiles were correlated with pneumonia severity index and microbial aetiology.
  
  
  RESULTS
  In the BAL of CAP patients, proportions of IL-17A and IL-22 single positive, as well as IL-17A/IL-22 double positive CD4 T cells were significantly increased compared with healthy individuals. Significantly increased proportions of IL-17A/IL-22 double positive CD4 T cells in BAL were found in non-severe and severe CAP patients, as well as in pneumococcal and non-pneumococcal CAP. In the peripheral blood of CAP patients upon admission, we found significantly increased proportions of IL-17A/IL-22 double positive CD4 T cells. One week after admission, the proportions of these double positive cells were still significantly increased in CAP patients compared with healthy individuals.
  
  
  CONCLUSIONS
  These data indicate that Th17 cells are engaged in the local and systemic immune response in human pneumonia. Especially, IL-17A/IL-22 double positive Th17 cells may be involved in the immunopathogenesis of CAP.
authors: Department of Pulmonary Medicine, Erasmus MC Rotterdam, Rotterdam, The Netherlands.
authors: Marthe S
authors: MS
authors: Paats
authors: Ingrid M
authors: IM
authors: Bergen
authors: Wessel E J J
authors: WE
authors: Hanselaar
authors: E Christine Groeninx
authors: EC
authors: van Zoelen
authors: Henry A
authors: HA
authors: Verbrugh
authors: Henk C
authors: HC
authors: Hoogsteden
authors: Bernt
authors: B
authors: van den Blink
authors: Rudi W
authors: RW
authors: Hendriks
authors: Menno M
authors: MM
authors: van der Eerden
mesh_terms: D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D017714:Community-Acquired Infections / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D005500:Follow-Up Studies; D006801:Humans; D007111:Immunity, Cellular; D020381:Interleukin-17 / Q000096:biosynthesis; D007378:Interleukins / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D011014:Pneumonia / Q000276:immunology* / Q000378:metabolism; D011446:Prospective Studies; D058504:Th17 Cells / Q000276:immunology*; D000097043:Interleukin-22
chemical_list: D020381:Interleukin-17; D007378:Interleukins; D000097043:Interleukin-22