title: FRET excited ratiometric oxygen sensing in living tissue.
abstract: Dynamic analysis of oxygen (O₂) has been limited by the lack of a real-time, quantitative, and biocompatible sensor. To address these demands, we designed a ratiometric optode matrix consisting of the phosphorescence quenching dye platinum (II) octaethylporphine ketone (PtOEPK) and nanocystal quantum dots (NQDs), which when embedded within an inert polymer matrix allows long-term pre-designed excitation through fluorescence resonance energy transfer (FRET). Depositing this matrix on various glass substrates allowed the development of a series of optical sensors able to measure interstitial oxygen concentration [O₂] with several hundred millisecond temporal resolution in varying biological microdomains of active brain tissue.
authors: Center for Neural Engineering, Department of Engineering Science and Mechanics, Pennsylvania State University, University Park, PA, USA. jui105@psu.edu
authors: Justin M
authors: JM
authors: Ingram
authors: Chunfeng
authors: C
authors: Zhang
authors: Jian
authors: J
authors: Xu
authors: Steven J
authors: SJ
authors: Schiff
mesh_terms: D015761:4-Aminopyridine / Q000494:pharmacology; D000818:Animals; D004396:Coloring Agents; D003199:Computer Systems; D003292:Convulsants / Q000494:pharmacology; D031541:Fluorescence Resonance Energy Transfer; D005898:Glass; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D053768:Metal Nanoparticles; D008839:Microelectrodes; D009944:Organoplatinum Compounds; D010092:Oximetry / Q000295:instrumentation / Q000379:methods*; D010101:Oxygen Consumption; D011166:Porphyrins; D017966:Pyramidal Cells / Q000737:chemistry* / Q000187:drug effects; D045663:Quantum Dots; D051381:Rats; D017207:Rats, Sprague-Dawley; D059010:Single-Cell Analysis
chemical_list: D004396:Coloring Agents; D003292:Convulsants; D009944:Organoplatinum Compounds; D011166:Porphyrins; C582846:platinum(II) octaethylporphine ketone; D015761:4-Aminopyridine