title: Selection of aptamers against Ara h 1 protein for FO-SPR biosensing of peanut allergens in food matrices.
abstract: The rising prevalence to food allergies in the past two decades, together with the fact that the only existing therapy is avoidance of allergen-containing food next to the implementation of anti-allergic drugs, urges the need for improved performance of current assays to detect potential allergens in food products. Therein, the focus has been on aptamer-based biosensors in recent years. In this paper we report for the first time the selection of aptamers against one of the most important peanut allergens, Ara h 1. Several Ara h1 DNA aptamers were selected after eight selection rounds using capillary electrophoresis (CE)-SELEX. The selected aptamers specifically recognized Ara h 1 and did not significantly bind with other proteins, including another peanut allergen Ara h 2. The dissociation constant of a best performing aptamer was in the nanomolar range as determined independently by three different approaches, which are surface plasmon resonance, fluorescence anisotropy, and capillary electrophoresis (353 ± 82 nM, 419 ± 63 nM, and 450 ± 60 nM, respectively). Furthermore, the selected aptamer was used for bioassay development on a home-built fiber optic surface plasmon resonance (FO-SPR) biosensor platform for detecting Ara h 1 protein in both buffer and food matrix samples demonstrating its real potential for the development of novel, more accurate aptamer-based biosensors. In conclusion, the reported aptamer holds a great potential for the detection of Ara h 1 in both the medical field and the food sector due to its high affinity and specificity for the target protein.
authors: BIOSYST-MeBioS, KU Leuven-University of Leuven, Willem de Croylaan 42, B-3001 Leuven, Belgium. ttdinh@hua.edu.vn
authors: Dinh T
authors: DT
authors: Tran
authors: Karel
authors: K
authors: Knez
authors: Kris P
authors: KP
authors: Janssen
authors: Jeroen
authors: J
authors: Pollet
authors: Dragana
authors: D
authors: Spasic
authors: Jeroen
authors: J
authors: Lammertyn
mesh_terms: D052179:Antigens, Plant / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D005504:Food Analysis / Q000295:instrumentation*; D005506:Food Contamination / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis*; D008565:Membrane Proteins; D010940:Plant Proteins / Q000032:analysis*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*
chemical_list: D052179:Antigens, Plant; D052157:Aptamers, Nucleotide; C500889:Ara h 1 protein, Arachis hypogaea; D006023:Glycoproteins; D008565:Membrane Proteins; D010940:Plant Proteins