title: Localization of multidomain adaptor proteins, p140Cap and vinexin, in the pancreatic islet of a spontaneous diabetes mellitus model, Otsuka Long-Evans Tokushima Fatty rats.
abstract: We have shown that two multidomain adaptor proteins, p140Cap and vinexin, interact with each other and are likely to be involved in neurotransmitter release. Because the basic molecular mechanism governing neurotransmitter and insulin secretion is conserved, these two proteins may also to play pivotal roles in insulin secretion. We therefore performed some characterization of p140Cap and vinexin in pancreas of a wild-type rat or a spontaneous type 2 diabetes mellitus (DM) model, the Otsuka Long-Evans Tokushima Fatty (OLETF) rat. These two proteins were detected in Wistar rat pancreas by Western blotting. Immunohistochemistry revealed that p140Cap and vinexin are enriched in β and α cells, respectively, in the rat pancreas. We then found that pancreatic islet structure was disorganized in the OLETF rat with hyperinsulinemia or with hyperglycemia, based on immunohistochemical analyses of vinexin. In β cells of these model rats, p140Cap was distributed in a cytoplasmic granular pattern as in the control rats, although its expression was reduced to various extents from cell to cell. These results may suggest possible involvement of p140Cap in insulin secretion, and reduction of p140Cap might be related to abnormal insulin secretion in DM.
authors: Department of General Internal Medicine, Gifu University School of Medicine, Gifu, Japan.
authors: Masahiro
authors: M
authors: Yamauchi
authors: Kaori
authors: K
authors: Sudo
authors: Hidenori
authors: H
authors: Ito
authors: Ikuko
authors: I
authors: Iwamoto
authors: Rika
authors: R
authors: Morishita
authors: Toshihiro
authors: T
authors: Murai
authors: Kazuo
authors: K
authors: Kajita
authors: Tatsuo
authors: T
authors: Ishizuka
authors: Koh-ichi
authors: K
authors: Nagata
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D001835:Body Weight; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D007150:Immunohistochemistry; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000378:metabolism*; D008297:Male; D018377:Neurotransmitter Agents / Q000378:metabolism; D051381:Rats; D020334:Rats, Inbred OLETF
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport; D007328:Insulin; D018377:Neurotransmitter Agents; C512725:Sorbs3 protein, rat; C000631270:Srcin1 protein, rat