title: Protein characterization of protein bodies from cotyledons of Mucuna pruriens (L.) DC.
abstract: Seeds of Mucuna pruriens (L.) DC. (Fabaceae) were analyzed for protein composition of protein bodies isolated from cotyledons. Protein bodies were successfully separated by Lympholyte and those of dry seeds, observed by scanning electron microscope, were elliptical or spherical in shape with a diameter of 5-12 μm. Protein content in dry seed protein bodies was 10.6 mg/g dry weight. Globulin was the largest protein fraction isolated (62.5 %), followed by albumin (18.3 %), glutelin (15.8 %) and prolamin (3.4 %). The prolamin fraction and high glutelin content are uncommon in legumes. SDS-PAGE of albumins, globulins, prolamins and glutelins provided different band numbers and molecular weights under reducing and non reducing conditions and suggested that the albumin fraction is rich in disulphide bonds.
authors: Dipartimento di Biologia Evolutiva, Università di Siena, Via A. Moro 2, 53100, Siena, Italy. bellani@unisi.it
authors: Lorenza
authors: L
authors: Bellani
authors: Stefania
authors: S
authors: Giglioni
authors: Simonetta
authors: S
authors: Muccifora
mesh_terms: D000418:Albumins / Q000032:analysis* / Q000378:metabolism; D018548:Cotyledon / Q000737:chemistry* / Q000378:metabolism; D004044:Dietary Proteins / Q000032:analysis; D005916:Globulins / Q000032:analysis* / Q000378:metabolism; D005983:Glutens / Q000032:analysis* / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D031299:Mucuna / Q000737:chemistry* / Q000378:metabolism; D010665:Phenylpropanolamine / Q000032:analysis* / Q000378:metabolism; D055314:Seed Storage Proteins / Q000032:analysis* / Q000378:metabolism
chemical_list: D000418:Albumins; D004044:Dietary Proteins; D005916:Globulins; D055314:Seed Storage Proteins; D010665:Phenylpropanolamine; D005983:Glutens