title: Exploring chloroplastic changes related to chilling and freezing tolerance during cold acclimation of pea (Pisum sativum L.).
abstract: Pea (Pisum sativum L.) productivity is linked to its ability to cope with abiotic stresses such as low temperatures during fall and winter. In this study, we investigate the chloroplast-related changes occurring during pea cold acclimation, in order to further lead to genetic improvement of its field performance. Champagne and Térèse, two pea lines with different acclimation capabilities, were studied by physiological measurements, sub-cellular fractionation followed by relative protein quantification and two-dimensional DIGE. The chilling tolerance might be related to an increase in protein related to soluble sugar synthesis, antioxidant potential, regulation of mRNA transcription and translation through the chloroplast. Freezing tolerance, only observed in Champagne, seems to rely on a higher inherent photosynthetic potential at the beginning of the cold exposure, combined with an early ability to start metabolic processes aimed at maintaining the photosynthetic capacity, optimizing the stoichiometry of the photosystems and inducing dynamic changes in carbohydrate and protein synthesis and/or turnover.
authors: Université Lille 1/INRA, UMR 1281, Stress Abiotiques et Différenciation des Végétaux cultivés, 59650 Villeneuve d'Ascq Cedex/Estrées-Mons, 80200 cedex, France; Centre de Recherche Public, Gabriel Lippmann, Department of Environment and Agrobiotechnologies (EVA), 4422, Belvaux, Luxembourg. Electronic address: grimaud@insa-toulouse.fr.
authors: Florent
authors: F
authors: Grimaud
authors: Centre de Recherche Public, Gabriel Lippmann, Department of Environment and Agrobiotechnologies (EVA), 4422, Belvaux, Luxembourg. Electronic address: renaut@lippmann.lu.
authors: Jenny
authors: J
authors: Renaut
authors: Université Lille 1/INRA, UMR 1281, Stress Abiotiques et Différenciation des Végétaux cultivés, 59650 Villeneuve d'Ascq Cedex/Estrées-Mons, 80200 cedex, France. Electronic address: estelle.dumont@univ-tln.fr.
authors: Estelle
authors: E
authors: Dumont
authors: Centre de Recherche Public, Gabriel Lippmann, Department of Environment and Agrobiotechnologies (EVA), 4422, Belvaux, Luxembourg. Electronic address: sergeant@lippmann.lu.
authors: Kjell
authors: K
authors: Sergeant
authors: Université Lille 1/INRA, UMR 1281, Stress Abiotiques et Différenciation des Végétaux cultivés, 59650 Villeneuve d'Ascq Cedex/Estrées-Mons, 80200 cedex, France. Electronic address: Anca.Lucau@univ-lille1.fr.
authors: Anca
authors: A
authors: Lucau-Danila
authors: Université Lille 1/INRA, UMR 1281, Stress Abiotiques et Différenciation des Végétaux cultivés, 59650 Villeneuve d'Ascq Cedex/Estrées-Mons, 80200 cedex, France. Electronic address: Anne-Sophie.Blervacq@univ-lille1.fr.
authors: Anne-Sophie
authors: AS
authors: Blervacq
authors: Université Lille 1/INRA, UMR 1281, Stress Abiotiques et Différenciation des Végétaux cultivés, 59650 Villeneuve d'Ascq Cedex/Estrées-Mons, 80200 cedex, France. Electronic address: Helene.Sellier@mons.inra.fr.
authors: Hélène
authors: H
authors: Sellier
authors: Université Lille 1/INRA, UMR 1281, Stress Abiotiques et Différenciation des Végétaux cultivés, 59650 Villeneuve d'Ascq Cedex/Estrées-Mons, 80200 cedex, France. Electronic address: Nasser.Bahrman@univ-lille1.fr.
authors: Nasser
authors: N
authors: Bahrman
authors: Université Lille 1/INRA, UMR 1281, Stress Abiotiques et Différenciation des Végétaux cultivés, 59650 Villeneuve d'Ascq Cedex/Estrées-Mons, 80200 cedex, France. Electronic address: lejeune@mons.inra.fr.
authors: Isabelle
authors: I
authors: Lejeune-Hénaut
authors: Université Lille 1/INRA, UMR 1281, Stress Abiotiques et Différenciation des Végétaux cultivés, 59650 Villeneuve d'Ascq Cedex/Estrées-Mons, 80200 cedex, France. Electronic address: Bruno.Delbreil@univ-lille1.fr.
authors: Bruno
authors: B
authors: Delbreil
authors: Université Lille 1/INRA, UMR 1281, Stress Abiotiques et Différenciation des Végétaux cultivés, 59650 Villeneuve d'Ascq Cedex/Estrées-Mons, 80200 cedex, France. Electronic address: estelle.goulas@univ-lille1.fr.
authors: Estelle
authors: E
authors: Goulas
mesh_terms: D000064:Acclimatization; D018533:Biomass; D002244:Carbon / Q000737:chemistry; D002734:Chlorophyll / Q000737:chemistry; D002736:Chloroplasts / Q000737:chemistry* / Q000235:genetics; D003080:Cold Temperature; D004579:Electron Transport; D015180:Electrophoresis, Gel, Two-Dimensional; D005615:Freezing; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D009584:Nitrogen / Q000737:chemistry; D018384:Oxidative Stress; D018532:Pisum sativum / Q000378:metabolism / Q000502:physiology*; D010777:Photochemistry; D010788:Photosynthesis; D045332:Photosystem II Protein Complex; D010940:Plant Proteins / Q000378:metabolism; D020543:Proteome; D040901:Proteomics; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D013347:Subcellular Fractions
chemical_list: D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D020543:Proteome; D012333:RNA, Messenger; D002734:Chlorophyll; D002244:Carbon; D009584:Nitrogen