title: Synthesis, characterization, interaction with DNA and cytotoxicity of Pd(II) and Pt(II) complexes containing pyridine carboxylic acid ligands.
abstract: Four metal complexes [Pd(L(1))Cl(2)·2H(2)O] (1), [Pt(L(1))Cl(2)·2H(2)O] (2), [Pd(L(2))Cl(2)·H(2)O] (3) and [Pt(L(2))Cl(2)·H(2)O] (4) (L(1) = 2,2'-bipyridyl-5,5'-dicarboxylic acid, L(2) = 2,2'-bipyridyl-4,4'-dicarboxylic acid) have been synthesized under hydrothermal conditions and fully characterized by IR and (1)H-NMR spectra, elemental analysis, and X-ray single crystal diffraction analysis. Interactions of these complexes with fish sperm DNA (FS-DNA) were investigated using UV-Vis absorption and fluorescence spectroscopic methods. Further insight was brought by quantum chemistry calculations by means of G03 package and taking B3LYP functional Lanl2dz Gen basis set. Agarose gel electrophoresis run on pBR322 plasmid DNA gave proof that all four complexes exhibit efficient DNA cleavage. Complexes 1-4 manifested cytotoxic specificity and a significant cancer cell inhibitory rate. Independent apoptosis tests under the light microscope, performed on hematoxylin-eosin stained HeLa cells, evidenced morphological changes induced by all the complexes.
authors: Laboratory of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang 110142, China. yaguangsun@yahoo.com.cn
authors: Ya-guang
authors: YG
authors: Sun
authors: Di
authors: D
authors: Sun
authors: Wan
authors: W
authors: Yu
authors: Ming-chang
authors: MC
authors: Zhu
authors: Fu
authors: F
authors: Ding
authors: Yi-nan
authors: YN
authors: Liu
authors: En-jun
authors: EJ
authors: Gao
authors: Shu-ju
authors: SJ
authors: Wang
authors: Gang
authors: G
authors: Xiong
authors: Ileana
authors: I
authors: Dragutan
authors: Valerian
authors: V
authors: Dragutan
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D053837:DNA Cleavage; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D009944:Organoplatinum Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D010165:Palladium / Q000737:chemistry* / Q000494:pharmacology; D011725:Pyridines / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
chemical_list: D056831:Coordination Complexes; D009944:Organoplatinum Compounds; D011725:Pyridines; D010165:Palladium; D004247:DNA