title: Identification and profiling of conserved and novel microRNAs from Chinese Qinchuan bovine longissimus thoracis.
abstract: BACKGROUND
  MicroRNAs (miRNAs) are a family of ~22 nucleotide small RNA molecules that regulate gene expression by fully or partially binding to their complementary sequences. Recently, a large number of miRNAs and their expression patterns have been identified in various species. However, to date no miRNAs have been reported to modulate muscle development in beef cattle.
  
  
  RESULTS
  Total RNAs from the Chinese Qinchuan bovine longissimus thoracis at fetal and adult stages were used to construct small RNA libraries for Solexa SBS technology sequencing. A total of 15,454,182 clean reads were obtained from the fetal bovine library and 13,558,164 clean reads from the adult bovine library. In total, 521 miRNAs including 104 novel miRNA candidates were identified. Furthermore, the nucleotide bias, base edit and family of the known miRNAs were also analyzed. Based on stem-loop qPCR, 25 high-read miRNAs were detected, and the results showed that bta-miRNA-206, miRNA-1, miRNA-133, miRNAn12, and miRNAn17 were highly expressed in muscle-related tissue or organs, suggesting that these miRNAs may play a role in the development of bovine muscle tissues.
  
  
  CONCLUSIONS
  This study confirmed the authenticity of 417 known miRNAs, discovered 104 novel miRNAs in bos taurus, and identified five muscle-specific miRNAs. The identification of novel miRNAs significantly expanded the repertoire of bovine miRNAs and could contribute to further studies on the muscle development of cattle.
authors: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, 712100, China.
authors: Jiajie
authors: J
authors: Sun
authors: Mijie
authors: M
authors: Li
authors: Zhuanjian
authors: Z
authors: Li
authors: Jing
authors: J
authors: Xue
authors: Xianyong
authors: X
authors: Lan
authors: Chunlei
authors: C
authors: Zhang
authors: Chuzhao
authors: C
authors: Lei
authors: Hong
authors: H
authors: Chen
mesh_terms: D000818:Animals; D002417:Cattle; D017124:Conserved Sequence; D053263:Gene Regulatory Networks; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000235:genetics*; D024510:Muscle Development / Q000235:genetics; D009132:Muscles / Q000378:metabolism*; D015203:Reproducibility of Results; D017423:Sequence Analysis, RNA
chemical_list: D035683:MicroRNAs