title: Mitochondrial metabolism transition cooperates with nuclear reprogramming during induced pluripotent stem cell generation.
abstract: Induced pluripotent stem cells (iPSCs) hold great clinical potential for regenerative medicine. Much work has been done to investigate the mechanisms of their generation, focusing on the cell nucleus. However, the roles of specific organelles and in particular mitochondria in the potential mechanisms of nuclear reprogramming remain unclear. In this study, we sought to determine the role of mitochondrial metabolism transition in nuclear reprogramming. We found that the mitochondrial cristae had remodeled in iPSCs. The efficiency of iPSC generation was significantly reduced by down-regulation of mitochondrial inner membrane protein (IMMT), which regulates the morphology of mitochondrial cristae. Moreover, cells with the oxidative phosphorylation (OXPHOS) advantage had higher reprogramming efficiency than normal cells and the glycolysis intermediate lactic acid enhanced the efficiency of iPSCs generation. Our results show that the remodeling of mitochondrial cristae couples with the generation of iPSCs, suggesting mitochondrial metabolism transition plays an important role in nuclear reprogramming.
authors: Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, PR China.
authors: Wenbo
authors: W
authors: Liu
authors: Qi
authors: Q
authors: Long
authors: Keshi
authors: K
authors: Chen
authors: Shengbiao
authors: S
authors: Li
authors: Ge
authors: G
authors: Xiang
authors: Shen
authors: S
authors: Chen
authors: Xiyin
authors: X
authors: Liu
authors: Yuxing
authors: Y
authors: Li
authors: Liang
authors: L
authors: Yang
authors: Delu
authors: D
authors: Dong
authors: Cheng
authors: C
authors: Jiang
authors: Zhenhua
authors: Z
authors: Feng
authors: Dajiang
authors: D
authors: Qin
authors: Xingguo
authors: X
authors: Liu
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D065150:Cellular Reprogramming; D006019:Glycolysis; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000648:ultrastructure; D051379:Mice; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D010085:Oxidative Phosphorylation
chemical_list: D024101:Mitochondrial Proteins; D009124:Muscle Proteins; C532603:mitofilin protein, mouse