title: Novel cellulase recycling method using a combination of Clostridium thermocellum cellulosomes and Thermoanaerobacter brockii β-glucosidase.
abstract: This report describes a novel recycling method utilizing a combination of Clostridium thermocellum cellulosomes and Thermoanaerobacter brockii β-glucosidase (CglT). To recover cellulosomes and CglT through re-binding to additional cellulose, a chimeric CBM3-CglT was created by fusing carbohydrate binding module (CBM3) from the scaffolding protein CipA into the N-terminal region of CglT. When a recycling test using cellulosomes and CBM3-CglT was performed on microcrystalline cellulose, the process was capable of 4 rounds of recycling (1%w/vcellulose/round). Although irreversible absorption of cellulosomes and CBM3-CglT into the residues was observed when ammonia-pretreated rice straw and delignified rice straw was used as substrates, a maximum of 2 and 4 recycling rounds (1%w/vglucan/round) were achieved, respectively, consistent with a 70% saccharification rate. This novel recycling method using cellulosomes and CBM3-CglT has great potential as an effective lignocellulose degradation system.
authors: Biological Resources and Post-harvest Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki 303-8686, Japan.
authors: Rattiya
authors: R
authors: Waeonukul
authors: Akihiko
authors: A
authors: Kosugi
authors: Panida
authors: P
authors: Prawitwong
authors: Lan
authors: L
authors: Deng
authors: Chakrit
authors: C
authors: Tachaapaikoon
authors: Patthra
authors: P
authors: Pason
authors: Khanok
authors: K
authors: Ratanakhanokchai
authors: Masayoshi
authors: M
authors: Saito
authors: Yutaka
authors: Y
authors: Mori
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002480:Cellulase / Q000302:isolation & purification*; D002482:Cellulose / Q000378:metabolism; D044603:Cellulosomes / Q000378:metabolism*; D048013:Acetivibrio thermocellus; D008565:Membrane Proteins / Q000378:metabolism*; D012275:Oryza; D059027:Recycling; D046968:Thermoanaerobacter / Q000201:enzymology*; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C080369:CipA protein, Clostridium; D008565:Membrane Proteins; D002482:Cellulose; D001617:beta-Glucosidase; D002480:Cellulase