title: Mimicking native extracellular matrix with phytic acid-crosslinked protein nanofibers for cardiac tissue engineering.
abstract: A functional scaffold fabricated is developed from natural polymers, favoring regeneration of the ischemic myocardium. Hemoglobin/gelatin/fibrinogen (Hb/gel/fib) nanofibers are fabricated by electrospinning and are characterized for morphology, scaffold composition, functional groups and hydrophilicity. It is hypothesized that ex vivo pretreatment of mesenchymal stem cells (MSCs) using 5-azacytidine and such a functional nanofibrous construct having a high oxygen-carrying potential could lead to enhanced cardiomyogenic differentiation of MSCs and result in superior biological and functional effects. The combination of a functional nanofibrous scaffold composed of natural polymers and crosslinked with a natural crosslinking agent, phytic acid, and stem cell biology may prove to be a novel therapeutic device for treatment of myocardial infarction.
authors: Health care and Energy Materials Laboratory, Nanoscience and Nanotechnology Initiative, National University of Singapore, Faculty of Engineering, 2 Engineering Drive 3, Singapore 117576.
authors: Rajeswari
authors: R
authors: Ravichandran
authors: Vera
authors: V
authors: Seitz
authors: Jayarama
authors: J
authors: Reddy Venugopal
authors: Radhakrishnan
authors: R
authors: Sridhar
authors: Subramanian
authors: S
authors: Sundarrajan
authors: Shayanti
authors: S
authors: Mukherjee
authors: Erich
authors: E
authors: Wintermantel
authors: Seeram
authors: S
authors: Ramakrishna
mesh_terms: D001374:Azacitidine / Q000494:pharmacology; D015415:Biomarkers / Q000378:metabolism; D040761:Biomimetic Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000737:chemistry*; D055664:Electrochemical Techniques; D005109:Extracellular Matrix / Q000737:chemistry; D005340:Fibrinogen / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009206:Myocardium / Q000166:cytology; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010833:Phytic Acid / Q000737:chemistry*; D018800:Thy-1 Antigens / Q000378:metabolism; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014336:Troponin / Q000378:metabolism
chemical_list: D015415:Biomarkers; D003432:Cross-Linking Reagents; D006454:Hemoglobins; D018800:Thy-1 Antigens; D014336:Troponin; D010833:Phytic Acid; D005780:Gelatin; D005340:Fibrinogen; D001374:Azacitidine