title: Pedialyte promotes periodontal ligament cell survival and motility.
abstract: BACKGROUND
  The search still continues to find the best storage media for avulsed teeth. Unfortunately, some of the recommended storage solutions are not commonly found in households or do not preserve the periodontal ligament (PDL) cells long-term. The purpose of the present study was to determine whether Pedialyte is a viable alternative storage solution for avulsed teeth by assessing its ability to preserve human PDL cell viability.
  
  
  METHODS
  Human PDL cells were exposed to 6 different storage solutions (minimal essential medium [MEMα], Hank's balanced salt solution [HBSS], non-fat milk, coconut water, Pedialyte, or tap water) for 2, 6, 24, or 48 hours at 4°C or 25°C. Cell viability was quantified immediately or 1 week after exposure. The effects of these storage solutions on PDL cell motility and bacterial proliferation were also examined. The results were statistically analyzed by analysis of variance.
  
  
  RESULTS
  Pedialyte at 4°C and 25°C showed significantly (P < .001) higher cell survival compared with water after all time intervals. No significant difference was noted between control (MEMα), HBSS, coconut water, and Pedialyte at 4°C after 2 hours. Cells stored in Pedialyte for 24 hours at 25°C and assayed 1 week later showed significantly higher cell survivability compared with milk. Pedialyte supported significantly less bacterial growth compared with non-fat milk and coconut water. No difference in cell motility was observed for cells stored for 24 hours in Pedialyte, MEMα, HBSS, milk, or coconut water.
  
  
  CONCLUSIONS
  Pedialyte is a viable alternative as a storage solution for avulsed teeth.
authors: Department of Endodontics, Louisiana State University Health Science Center, School of Dentistry, New Orleans, Louisiana 70119, USA.
authors: Sara
authors: S
authors: Macway-Gomez
authors: Thomas E
authors: TE
authors: Lallier
mesh_terms: D000818:Animals; D001419:Bacteria / Q000254:growth & development; D001628:Beverages; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003059:Cocos; D003470:Culture Media; D006801:Humans; D007552:Isotonic Solutions / Q000627:therapeutic use; D008892:Milk; D019188:Organ Preservation Solutions / Q000627:therapeutic use*; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D028321:Plant Preparations / Q000627:therapeutic use; D015532:Rehydration Solutions / Q000627:therapeutic use*; D013696:Temperature; D013997:Time Factors; D046509:Tissue Culture Techniques; D014084:Tooth Avulsion / Q000628:therapy*; D014867:Water
chemical_list: D003470:Culture Media; C072670:Hanks Balanced Salt Solution; D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; D028321:Plant Preparations; D015532:Rehydration Solutions; D014867:Water