title: Low molecular weight PEI-based biodegradable lipopolymers as gene delivery vectors.
abstract: Non-viral gene vectors play an important role in the development of gene therapy. In this report, different hydrophobic chains were introduced into low molecular weight (LMW) PEI-based biodegradable oligomers to form a series of lipopolymers (LPs), and their structure-activity relationships were studied. Results revealed that the nine polymers can condense plasmid DNA well to form nanoparticles with appropriate sizes (120-250 nm) and positive zeta-potentials (+25-40 V). In vitro experiments were carried out and it was found that LP2 showed much higher transfection efficiency both in the presence and in the absence of serum under the polymer/DNA weight ratio of 0.8 in A549 cells.
authors: Key Laboratory of Green Chemistry and Technology (Ministry of Education), College of Chemistry, West China Hospital, Sichuan University, Chengdu 610064, People's Republic of China.
authors: Miao-Miao
authors: MM
authors: Xun
authors: Xue-Chao
authors: XC
authors: Zhang
authors: Ji
authors: J
authors: Zhang
authors: Qian-Qian
authors: QQ
authors: Jiang
authors: Wen-Jing
authors: WJ
authors: Yi
authors: Wen
authors: W
authors: Zhu
authors: Xiao-Qi
authors: XQ
authors: Yu
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D015394:Molecular Structure; D008970:Molecular Weight; D011094:Polyethyleneimine / Q000737:chemistry* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D011108:Polymers; D011094:Polyethyleneimine; D004247:DNA