title: Intracytoplasmic sperm injection improves in vitro embryo production from poor quality bovine oocytes.
abstract: The objective was to use subzonal sperm injection (SUZI) to understand sperm penetration patterns and to use intracytoplasmic sperm injection (ICSI) to improve production of bovine embryos using poor quality gametes. In experiment 1, poor versus good quality oocytes were fertilized with sperm from two bulls, A and B, with poor and good sperm vigor, respectively. The blastocyst rate was higher for good versus poor quality oocytes (23.3% vs. 11.1%, P < 0.05), regardless of the bull used. There was no significant difference in blastocyst rate for bull A (low vigor) regardless of oocyte quality, and for bull B (high vigor), blastocyst rate was better for good versus poor quality oocytes (25.7% vs. 9.2%, P < 0.05). In experiment 2, poor quality oocytes were subjected to SUZI. The oocyte penetration rate was lower for bull A than for bull B (29.6% vs. 53.8%, P < 0.05), when SUZI was performed within 1 hour after sperm processing. However, when SUZI was performed 2 to 3 hours after sperm processing, penetrating capacity was similar between bulls, but for bull B, penetrating capacity significantly decreased after 3 hours of sperm processing. In an attempt to overcome sperm penetrating disorders, poor and good quality oocytes were subjected to ICSI (experiment 3). Irrespective of the bull or of the oocyte quality grade, there were no differences in cleavage or blastocyst rates. Both bulls had distinct IVF embryo production rates, which we inferred were because of particular individual sperm characteristics. In conclusion, ICSI was an effective means to achieve in vitro production of bovine embryos with gametes of variable quality.
authors: Laboratório de Reprodução Animal prof. Assis Roberto de Bem, Universidade do Estado de Santa Catarina-UDESC, Lages, Santa Catarina, Brazil.
authors: L U
authors: LU
authors: Ohlweiler
authors: D S
authors: DS
authors: Brum
authors: F G
authors: FG
authors: Leivas
authors: A B
authors: AB
authors: Moyses
authors: R S
authors: RS
authors: Ramos
authors: N
authors: N
authors: Klein
authors: J C
authors: JC
authors: Mezzalira
authors: A
authors: A
authors: Mezzalira
mesh_terms: D000818:Animals; D002417:Cattle / Q000502:physiology*; D047108:Embryonic Development; D005260:Female; D008297:Male; D009865:Oocytes / Q000502:physiology*; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods / Q000662:veterinary*