title: Findings in detection of Herpesviridae by polymerase chain reaction and intraocular antibody production in a case series of anterior uveitis.
abstract: OBJECTIVE
  To target the use of two biologic tests in the diagnostic of viral Herpesviridae anterior uveitis (AC) by the consideration of clinical behavior and delay of intraocular sampling.
  
  
  METHODS
  Aqueous humor samples were collected from 42 patients suspected of having AU of infectious origin at presentation. The diagnosis of infectious uveitis was confirmed by quantification of antibodies with the Goldmann-Witmer coefficient (GWC) and/or detection of Herpesviridae genomes with PCR. The data were compared with data of 16 uveitis control samples used to calculate the specificity of the tests.
  
  
  RESULTS
  Sixteen out of 42 eyes (38%) had a final diagnosis of anterior segment infectious uveitis of viral origin (Herpesviridae) confirmed by PCR positive result (5/14 eyes; 14 of the 16 eyes were tested by PCR) and/or specific intraocular antibody synthesis (14/16 eyes).
  
  
  CONCLUSIONS
  While the GWC is progressively less often performed, these findings suggest that it still has a role in AU suspected of herpesvirus etiology.
authors: Service d'Ophtalmologie IV, Centre National d'Ophtalmologie des Quinze-Vingts, Paris, France. marie-helene.errera@orange.fr
authors: Marie-Hélène
authors: MH
authors: Errera
authors: Pablo
authors: P
authors: Goldschmidt
authors: Laurence
authors: L
authors: Batellier
authors: Sandrine
authors: S
authors: Degorge
authors: Emmanuel
authors: E
authors: Héron
authors: Laurent
authors: L
authors: Laroche
authors: José-Alain
authors: JA
authors: Sahel
authors: Mark
authors: M
authors: Westcott
authors: Christine
authors: C
authors: Chaumeil
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis*; D000917:Antibody Formation; D001082:Aqueous Humor / Q000276:immunology / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis*; D015828:Eye Infections, Viral / Q000175:diagnosis / Q000276:immunology / Q000821:virology*; D005260:Female; D006564:Herpesviridae / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D014606:Uveitis, Anterior / Q000175:diagnosis / Q000276:immunology / Q000821:virology*; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral