title: Antinociceptive and wound healing activities of Croton adamantinus Müll. Arg. essential oil.
abstract: Leaves of Croton adamantinus have been used to treat inflammation and skin wounds in the semi-arid area of the Northeast of Brazil. In order to evaluate if the essential oil (EO) was responsible for the claimed activities; antinociceptive, wound healing and antimicrobial tests were carried out. Twenty constituents were identified in C. adamantinus EO by GC-MS, ¹H-NMR and ¹³C-NMR, the major compounds being methyl-eugenol (14.81%) and 1,8-cineol (13.74%). Antinociceptive activity was evaluated by the formalin test and the abdominal contortion assay in mice. The EO (50 and 100 mg/kg) decreased the licking time of both phases of the formalin test when compared to the vehicle, but not to morphine (7.5 mg/kg). In the abdominal contortion assay, the EO (50 and 100 mg/kg) reduced the number of contortions compared to the vehicle and to indometacin (10 mg/kg). The wound healing activity was verified also using two experimental models: excisional wound and dead space. Topical treatment with the EO (1%) increased the wound contraction from the third day of treatment (compared with nitrofurazone 0.2%), while systemic treatment (50 mg/kg/day) increased granulation tissue formation and reduced the water content. C. adamantinus EO also showed antimicrobial activity against Staphylococcus aureus in disk diffusion method. These results corroborate the ethnobotanical use of this specie by Brazilian population.
authors: Department of Physiology and Pharmacology, Federal University of Ceará, Rua Coronel Nunes de Melo 1315, Fortaleza, CE, 60.430-270, Brazil, ximenesrm@gmail.com.
authors: Rafael Matos
authors: RM
authors: Ximenes
authors: Lenise
authors: L
authors: de Morais Nogueira
authors: Nathalia Maria Rodrigues
authors: NM
authors: Cassundé
authors: Roberta Jeane Bezerra
authors: RJ
authors: Jorge
authors: Simone Maria
authors: SM
authors: dos Santos
authors: Lucimere Paulino Machado
authors: LP
authors: Magalhães
authors: Monalisa Ribeiro
authors: MR
authors: Silva
authors: Glauce Socorro
authors: GS
authors: de Barros Viana
authors: Renata Mendonça
authors: RM
authors: Araújo
authors: Kêsia Xisto da Fonseca Ribeiro
authors: KX
authors: de Sena
authors: Julianna Ferreira Cavalcanti
authors: JF
authors: de Albuquerque
authors: René Duarte
authors: RD
authors: Martins
mesh_terms: D000700:Analgesics / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D001938:Brazil; D029802:Croton / Q000737:chemistry*; D003511:Cyclohexanols / Q000032:analysis; D000077591:Eucalyptol; D008297:Male; D051379:Mice; D039821:Monoterpenes / Q000032:analysis; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D010147:Pain Measurement; D018515:Plant Leaves / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000700:Analgesics; D003511:Cyclohexanols; D039821:Monoterpenes; D009822:Oils, Volatile; D010938:Plant Oils; D000077591:Eucalyptol