title: Isolation and complete genomic characterization of pandemic H1N1/2009 influenza viruses from Cuban swine herds.
abstract: The emergence of the pandemic H1N1/2009 influenza virus poses a potential global threat for human and animal health. In this study, we carried out pandemic H1N1/2009 influenza virus surveillance in swine herds in Cuba intending to determine whether the virus was circulating among pig populations. As a result we describe, for the first time, the detection of pandemic H1N1/2009 influenza virus in swine herds in Cuba. In addition, phylogenetic analysis and molecular characterization of three viral isolates were performed. Phylogenetic relationships confirmed that all of the eight genes of the three isolates were derived from the pandemic H1N1/2009 virus. The Cuban isolates, formed an independent cluster within the pandemic H1N1/2009 influenza strains. Different molecular markers, previously described in pandemic H1N1/2009 influenza viruses, related with adaptive evolution, viral evasion from the host-immune response, virulence and dissemination were also present in Cuban pandemic H1N1/2009 isolates.
authors: Centro Nacional de Sanidad Agropecuaria (CENSA), La Habana, Cuba.
authors: Lester Josué
authors: LJ
authors: Pérez
authors: Carmen Laura
authors: CL
authors: Perera
authors: Armando
authors: A
authors: Vega
authors: Maria T
authors: MT
authors: Frías
authors: Dagmar
authors: D
authors: Rouseaux
authors: Llilianne
authors: L
authors: Ganges
authors: José Ignacio
authors: JI
authors: Nuñez
authors: Heidy
authors: H
authors: Díaz de Arce
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003462:Cuba / Q000453:epidemiology; D016679:Genome, Viral / Q000235:genetics*; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009976:Orthomyxoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D058873:Pandemics; D010802:Phylogeny; D016415:Sequence Alignment / Q000662:veterinary; D013552:Swine / Q000821:virology; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*