title: Analysis of polymorphisms and haplotypes in genes associated with vascular tone, hypertension and oxidative stress in Mexican-Mestizo women with severe preeclampsia.
abstract: OBJECTIVE
  Several studies have reported the association of genes related to vascular tone, hypertension, oxidative stress and preeclampsia. We investigated the possible association among three polymorphisms in eNOS (as well their haplotypes): one of MTHFR, one of GSTP1 and one of AGT, with severe preeclampsia in Mexican-Mestizo women.
  
  
  METHODS
  Two hundred thirty women with severe preeclampsia and 350 control subjects were genotyped; for rs2070744 and rs1799983 of eNOS, rs1801133 of MTHFR, rs1695 of GSTP1 and rs699 of AGT we used real-time PCR allelic discrimination and for VNTR of eNOS, PCR. Allele frequency differences were assessed by χ(2). Logistic regression was used to test for associations and for haplotype frequencies using Haploview 4.2.
  
  
  RESULTS
  Genotypic and allelic distribution of the polymorphisms was similar between cases and controls; likewise, haplotype frequencies of the three polymorphisms of eNOS did not differ significantly.
  
  
  CONCLUSIONS
  To our knowledge, this is the first time that these polymorphisms have been analyzed together and exclusively in women with severe preeclampsia. However, we did not find an association between polymorphisms of eNOS, MTHFR, GSTP1 and AGT with severe preeclampsia in our population. Additionally, we observed differences in the distribution of the alleles and genotypes of these polymorphisms in our population in comparison to those described in other ethnic groups.
authors: Sección de Posgrado, Escuela Superior de Medicina, Instituto Politécnico Nacional, México, D.F., Mexico.
authors: Ramón Mauricio
authors: RM
authors: Coral-Vázquez
authors: Juan Fernando
authors: JF
authors: Romero Arauz
authors: Samuel
authors: S
authors: Canizales-Quinteros
authors: Agustín
authors: A
authors: Coronel
authors: Elith Yazmin
authors: EY
authors: Valencia Villalvazo
authors: Jaime
authors: J
authors: Hernández Rivera
authors: Belem
authors: B
authors: Ramírez Regalado
authors: David
authors: D
authors: Rojano Mejía
authors: Patricia
authors: P
authors: Canto
mesh_terms: D000328:Adult; D000808:Angiotensinogen / Q000235:genetics; D005260:Female; D051549:Glutathione S-Transferase pi / Q000235:genetics; D006239:Haplotypes; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics; D008800:Mexico; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D018384:Oxidative Stress / Q000235:genetics*; D011110:Polymorphism, Genetic; D011225:Pre-Eclampsia / Q000208:ethnology / Q000235:genetics*; D011247:Pregnancy
chemical_list: D000808:Angiotensinogen; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III; D042965:Methylenetetrahydrofolate Reductase (NADPH2); D051549:Glutathione S-Transferase pi