title: Alkylphosphocholines for intraocular lens coating.
abstract: OBJECTIVE
  To assess the effect of alkylphosphocholine (APC)-coated intraocular lenses (IOLs) on human lens epithelial cell (LEC) proliferation in vitro and the corneal biocompatibility of APC in an organ culture model of human donor eyes.
  
  
  METHODS
  Research Laboratory for Experimental Ophthalmology, Ludwig-Maximilians-University, Munich, Germany.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  Six foldable IOLs differing in optic material were incubated with APC, washed in phosphate-buffered saline, and dried overnight. Intraocular lenses of the same lot served as uncoated controls. Each rehydrated IOL was placed in 1 well of a 24-well plate containing proliferating human LECs. Cell survival was tested by the tetrazolium-dye reduction assay 5 days later. Biocompatibility of the human corneal endothelium was assessed by a 24-hour incubation of human donor corneas with different concentrations of APC before the live/dead assay was performed.
  
  
  RESULTS
  Human LEC proliferation was attenuated by APC-coated IOLs (P=.001). Coated hydrophilic acrylic IOLs were more effective inhibitors of human LEC proliferation than coated hydrophobic acrylic or silicone IOLs (P=.001). Alkylphosphocholines were well tolerated by the corneal endothelium in an organ culture model of human donor corneas up to a concentration of 1 mM (n = 12).
  
  
  CONCLUSIONS
  Results show that APCs are suitable agents for IOL coating without linker molecules. Coated IOLs can inhibit human LEC proliferation and were well tolerated by human donor corneas in organ culture.
authors: Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany. kirsten.eibl@med.uni-muenchen.de
authors: Kirsten H
authors: KH
authors: Eibl
authors: Christian
authors: C
authors: Wertheimer
authors: Marcus
authors: M
authors: Kernt
authors: Armin
authors: A
authors: Wolf
authors: Daniel
authors: D
authors: Kook
authors: Christos
authors: C
authors: Haritoglou
authors: Anselm
authors: A
authors: Kampik
mesh_terms: D000180:Acrylic Resins; D002452:Cell Count; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival; D020099:Coated Materials, Biocompatible; D004728:Endothelium, Corneal / Q000473:pathology*; D004847:Epithelial Cells / Q000473:pathology*; D006801:Humans; D007908:Lens, Crystalline / Q000473:pathology*; D007910:Lenses, Intraocular; D009924:Organ Culture Techniques; D010767:Phosphorylcholine / Q000031:analogs & derivatives / Q000494:pharmacology*; D014019:Tissue Donors
chemical_list: D000180:Acrylic Resins; D020099:Coated Materials, Biocompatible; D010767:Phosphorylcholine