title: Effect of Staphylococcus aureus and Staphylococcus epidermidis debris and supernatant on bone marrow stromal cells growth.
abstract: OBJECTIVE
  the goal of this study is to observe the effect of SA and SE debris and supernatant on BMSCs growth.
  
  
  METHODS
  SA and SE were isolated and cultured from lesion materials of spondylitis patients which were determined by gram staining and biochemical tests. BMSCs were cultured and incubated for 11 days to be further sub-cultured and tripsinized for cell counting before seeded. 0.1 mg/ml SA and SE debris and supernatant were added into the BMSCs culture media. Cell counting was performed 2, 5, 7, and 9 days after debris and supernatant addition to get the growth profile of BMSCs.
  
  
  RESULTS
  debris and supernatant decreases BMSCS number at initial day. At day 5, BMSCs in the group debris were growing down, mean while BMSCs in the group supernatant were able to retain the cell number. Viability of all groups was more than 80%.
  
  
  CONCLUSIONS
  both debris and supernatant from SA and SE have inhibitory effect of the growth BMSCs in the initial day. BMSCs could provide barrier to survive from the debris and supernatant environment of both bacteria in day five. BMSCs can use supernatant to retain the growth to replace the lack of nutrition.
authors: Department of Orthopaedic and Traumatology, Faculty of Medicine, University of Indonesia - Cipto Mangunkusumo Hospital. Jl. Diponegoro no. 71, Jakarta Pusat 10430, Indonesia. rahyussalim@gmail.com
authors: Rahyussalim
authors: Andriansjah
authors: Yuyus
authors: Y
authors: Kusnadi
authors: Hd
authors: H
authors: Ismail
authors: Andri
authors: A
authors: Lubis
authors: Tri
authors: T
authors: Kurniawati
authors: Maurin
authors: M
authors: Merlina
mesh_terms: D002452:Cell Count; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D013211:Staphylococcus aureus / Q000502:physiology*; D013212:Staphylococcus epidermidis / Q000502:physiology*