title: In vivo deglycosylation of recombinant proteins in plants by co-expression with bacterial PNGase F.
abstract: At present, several eukaryotic expression systems including yeast, insect and mammalian cells and plants are used for the production of recombinant proteins. Proteins with potential N-glycosylation sites are efficiently glycosylated when expressed in these systems. However, the ability of the eukaryotic expression systems to glycosylate may be not desirable for some proteins. If target proteins that do not carry N-linked glycans in the native host contain potential N-linked glycosylation sites, they can be aberrantly glycosylated in the eukaryotic expression systems, thus, potentially impairing biological activity. Recently, we have developed a strategy of enzymatic deglycosylation of proteins in vivo by co-introducing bacterial PNGase F via agroinfiltration followed by transient expression in plants. (1) Here, we summarize our work on this topic and its potential implications.
authors: Fraunhofer USA Center for Molecular Biotechnology; Newark, DE USA.
authors: Tarlan
authors: T
authors: Mamedov
authors: Vidadi
authors: V
authors: Yusibov
mesh_terms: D000818:Animals; D022122:Anthrax Vaccines / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D005417:Flavobacterium / Q000737:chemistry* / Q000201:enzymology; D015870:Gene Expression; D006031:Glycosylation; D006801:Humans; D017780:Malaria Vaccines / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism
chemical_list: D022122:Anthrax Vaccines; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D017780:Malaria Vaccines; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
keywords: N-linked glycosylation; PNGase F; deglycosylation; malaria vaccine candidate Pfs48/45; plant transient expression; recombinant proteins