title: Comparative proteomic analysis of Puccinellia tenuiflora leaves under Na2CO3 stress.
abstract: Soil salt-alkalinization is a widespread environmental stress that limits crop growth and agricultural productivity. The influence of soil alkalization caused by Na(2)CO(3) on plants is more severe than that of soil salinization. Plants have evolved some unique mechanisms to cope with alkali stress; however, the plant alkaline-responsive signaling and molecular pathways are still unknown. In the present study, Na(2)CO(3) responsive( )characteristics in leaves from 50-day-old seedlings of halophyte Puccinellia tenuiflora were investigated using physiological and proteomic approaches. Comparative proteomics revealed 43 differentially expressed proteins in P. tenuiflora leaves in response to Na(2)CO(3) treatment for seven days. These proteins were mainly involved in photosynthesis, stress and defense, carbohydrate/energy metabolism, protein metabolism, signaling, membrane and transport. By integrating the changes of photosynthesis, ion contents, and stress-related enzyme activities, some unique Na(2)CO(3) responsive( )mechanisms have been discovered in P. tenuiflora. This study provides new molecular information toward improving the alkali tolerance of cereals.
authors: College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China. daishaojun@hotmail.com.
authors: Juanjuan
authors: J
authors: Yu
authors: Sixue
authors: S
authors: Chen
authors: Tai
authors: T
authors: Wang
authors: Guorong
authors: G
authors: Sun
authors: Shaojun
authors: S
authors: Dai
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D002254:Carbonates / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D016000:Cluster Analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D007477:Ions / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009997:Osmotic Pressure / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D010788:Photosynthesis / Q000187:drug effects; D018515:Plant Leaves / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000145:classification / Q000378:metabolism*; D006109:Poaceae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D020543:Proteome / Q000145:classification / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D055051:Salt-Tolerant Plants / Q000187:drug effects / Q000378:metabolism; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002254:Carbonates; D007477:Ions; D010940:Plant Proteins; D020543:Proteome; C005686:sodium carbonate; D008315:Malondialdehyde; D009195:Peroxidase; D013482:Superoxide Dismutase