title: Expression of tight junction proteins by umbilical vein epithelial cells co-cultured with allogenic astrocytes.
abstract: We studied expression of tight junction proteins and formation of the barrier properties in the culture of umbilical vein endothelial cells under conditions of co-culturing with allogenic GFAP-positive astrocytes. This culturing significantly increases of expression of tight junction proteins (claudin-5, occludin, and ZO-1). The formation of tight junctions significantly increased transendothelial resistance and reduced permeability for sodium fluorescein. Thus, reproducible in vitro model for the study of endothelial barrier properties was created based on co-culturing of umbilical endothelial cells and human astrocytes. This model can be used for evaluation of the permeability of the tissue-blood barriers for substances of different chemical structure and for studies of factors modulating the state of cell-cell contacts.
authors: Department of Medical Nanotechnologies, Medico-Biological Faculty, N. I. Pirogov Russian National Research Medical University, Ministry of Health Care and Social Development, Moscow, Russia. volginadi@gmail.com
authors: N E
authors: NE
authors: Volgina
authors: O I
authors: OI
authors: Gurina
authors: N F
authors: NF
authors: Grinenko
authors: V P
authors: VP
authors: Baklaushev
authors: N V
authors: NV
authors: Ivanova
authors: V P
authors: VP
authors: Chekhonin
mesh_terms: D001253:Astrocytes / Q000378:metabolism*; D001812:Blood-Brain Barrier / Q000378:metabolism; D002478:Cells, Cultured; D062507:Claudin-5 / Q000096:biosynthesis; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis; D006801:Humans; D062793:Occludin / Q000096:biosynthesis; D062725:Tight Junction Proteins / Q000096:biosynthesis*; D019108:Tight Junctions / Q000378:metabolism*; D014471:Umbilical Veins / Q000166:cytology* / Q000378:metabolism; D062826:Zonula Occludens-1 Protein / Q000096:biosynthesis
chemical_list: D062507:Claudin-5; D005904:Glial Fibrillary Acidic Protein; D062793:Occludin; C569042:TJP1 protein, human; D062725:Tight Junction Proteins; D062826:Zonula Occludens-1 Protein