title: Radioprotective effect of sodium selenite on bone repair in the tibia of ovariectomized rats.
abstract: This study evaluated protection by selenium (Se) in the bone repair process in ovariectomized rats after irradiation. For such purpose, 80 ovariectomized female Wistar rats were randomly divided into 4 experimental groups: ovariectomized (Ov), Ov/Se, Ov/irradiated (Irr) and Ov/ Se/Irr. A bone defect was created on the tibia of all animals 40 days after ovariectomy. Two days after surgery, only the Ov/Se and Ov/Se/Irr rats received 0.8 mg Se/kg. Three days after surgery, only the Ov/Irr and Ov/Se/Irr rats received 10 Gy of x-rays on the lower limb region. The animals were euthanized at 7, 14, 21 and 28 days after surgery to assess the repair process, which was evaluated by analysis of trabecular bone number (Masson Trichrome) and birefringence analysis (Picrosirius). It was possible to observe a delay in the bone repair process in the ovariectomized/irradiated group and similarity between the ovariectomized, Ov/Se and Ov/Se/Irr groups. In conclusion, sodium selenite exerted a radioprotective effect in the bone repair of tibia of ovariectomized rats without toxicity.
authors: Oral Radiology Area, Department of Oral Diagnosis, Piracicaba Dental School, UNICAMP - University of Campinas, Piracicaba, SP, Brazil. deborah@fop.unicamp.br
authors: Deborah Queiroz
authors: DQ
authors: de Freitas
authors: Flávia Maria de Moraes
authors: FM
authors: Ramos-Perez
authors: Ellen Gaby
authors: EG
authors: Neves
authors: Marcelo Rocha
authors: MR
authors: Marques
authors: Frab Norberto
authors: FN
authors: Bóscolo
authors: Solange Maria
authors: SM
authors: de Almeida
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001391:Azo Compounds; D015519:Bone Density / Q000187:drug effects / Q000528:radiation effects; D001847:Bone Diseases / Q000503:physiopathology; D001861:Bone Regeneration / Q000187:drug effects* / Q000528:radiation effects; D016723:Bone Remodeling / Q000187:drug effects / Q000528:radiation effects; D004396:Coloring Agents; D004801:Eosine Yellowish-(YS); D005260:Female; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008739:Methyl Green; D010052:Ovariectomy; D011829:Radiation Dosage; D011837:Radiation-Protective Agents / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020887:Selenious Acid / Q000627:therapeutic use*; D013977:Tibia / Q000187:drug effects* / Q000528:radiation effects; D013997:Time Factors
chemical_list: D000975:Antioxidants; D001391:Azo Compounds; D004396:Coloring Agents; D011837:Radiation-Protective Agents; C017768:trichrome stain; C009798:C.I. direct red 80; D008739:Methyl Green; D020887:Selenious Acid; D004801:Eosine Yellowish-(YS)