title: Cell membrane proteins from oviductal epithelial cell line protect human spermatozoa from oxidative damage.
abstract: OBJECTIVE
  To study the potential protective action in vitro of oviductal epithelial cell membrane proteins against oxidative damage in human spermatozoa.
  
  
  METHODS
  Prospective in vitro study.
  
  
  METHODS
  University research laboratory and infertility clinic.
  
  
  METHODS
  Semen from men attending the infertility clinic at the Queen Mary Hospital with normal semen parameters (World Health Organization, 2010).
  
  
  METHODS
  We studied the effect of oviductal epithelial cell membrane proteins on the sperm functions and endogenous antioxidant enzyme activities.
  
  
  METHODS
  Sperm motility, lipid peroxidation, DNA fragmentation, intracellular reactive oxygen species (ROS) level, superoxide dismutase, and glutathione peroxidase activities.
  
  
  RESULTS
  Oviductal epithelial cell membrane proteins bind to the human spermatozoa and protect them from ROS-induced damages in terms of sperm motility, membrane integrity, DNA integrity, and intracellular ROS level. Spermatozoa-oviduct epithelial cell interaction also enhances the antioxidant defenses in spermatozoa.
  
  
  CONCLUSIONS
  Our results demonstrated the protective effects of spermatozoon-oviductal epithelial cell interaction against oxidative stress in human spermatozoa. The results enhance our understanding of the protective mechanism of oviduct on sperm functions.
authors: Department of Obstetrics and Gynecology, University of Hong Kong, Queen Mary Hospital, Hong Kong, PR China.
authors: Venus W
authors: VW
authors: Huang
authors: Weie
authors: W
authors: Zhao
authors: Cheuk-Lun
authors: CL
authors: Lee
authors: Cherie Y L
authors: CY
authors: Lee
authors: Kevin K W
authors: KK
authors: Lam
authors: Jennifer K Y
authors: JK
authors: Ko
authors: William S B
authors: WS
authors: Yeung
authors: Pak-Chung
authors: PC
authors: Ho
authors: Philip C N
authors: PC
authors: Chiu
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D002450:Cell Communication / Q000502:physiology*; D002461:Cell Line, Transformed; D053938:DNA Fragmentation; D004847:Epithelial Cells / Q000378:metabolism; D005187:Fallopian Tubes / Q000166:cytology* / Q000378:metabolism*; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D011485:Protein Binding / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013075:Sperm Capacitation / Q000502:physiology; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D008565:Membrane Proteins; D017382:Reactive Oxygen Species; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase