title: 19-VNTR loci used in genotyping Chinese clinical Mycobacterium tuberculosis complex strains and in association with spoligotyping.
abstract: Recently, tandem repeat typing has emerged as a rapid and easy method for the molecular epidemiology of the Mycobacterium tuberculosis (M. tuberculosis) complex. In this study, a collection of 19 VNTRs incorporating 15 previously described loci and 4 newly evaluated markers were used to genotype 206 Chinese M. tuberculosis isolates and 9 BCG strains. The discriminatory power was evaluated and compared with that obtained by Spoligotyping. It turned out that 15-locus VNTR could be very useful in M. tuberculosis complex strains genotyping in China. The 4 newly evaluated loci were proved informative and could be useful for future epidemiology studies, especially in Beijing family strains. In addition, a unique pattern of the latter 4 loci were found in Chinese BCG strains.
authors: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention/State Key Laboratory for Infectious Disease Prevention and Control, Beijing, PR China.
authors: Yi
authors: Y
authors: Jiang
authors: Hai-can
authors: HC
authors: Liu
authors: Huajun
authors: H
authors: Zheng
authors: Xiangfeng
authors: X
authors: Dou
authors: Biao
authors: B
authors: Tang
authors: Xiu-qin
authors: XQ
authors: Zhao
authors: Yongqiang
authors: Y
authors: Zhu
authors: Bing
authors: B
authors: Lu
authors: Shengyue
authors: S
authors: Wang
authors: Hai-yan
authors: HY
authors: Dong
authors: Yuan-yuan
authors: YY
authors: Zhang
authors: Guoping
authors: G
authors: Zhao
authors: Kanglin
authors: K
authors: Wan
mesh_terms: D015373:Bacterial Typing Techniques; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry; D056426:Genetic Loci; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D014397:Tuberculosis, Pulmonary / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial
keywords: Mycobacterium tuberculosi; VNTR