title: Neutralization of complement component C5 ameliorates the development of dextran sulfate sodium (DSS)-colitis in mice.
abstract: The complement system is a potent effector of innate immunity. To elucidate the pathophysiological role of the complement system in inflammatory bowel disease, we evaluated the effects of anti-C5 antibodies on the development of dextran sulfate sodium-induced colitis in mice. Dextran sulfate sodium-colitis was induced in BALB/c mice with intraperitoneal administrations of anti-C5 antibodies (1 mg/body [DOSAGE ERROR CORRECTED]) every 48 h. Tissue samples were evaluated by standard histological procedures. The mucosal mRNA expression of the inflammatory cytokines was analyzed by real-time PCR. Body weight loss in the mice was completely blocked by the administration of anti-C5 antibody. The disease activity index was significantly lower in the anti-C5 antibody-treated mice than the dextran sulfate sodium mice. The colonic weight/length ratio, histological colitis score and mucosal myeloperoxidase activity were significantly lower in the anti-C5 antibody-treated mice than the dextran sodium sulfate mice. The administration of the anti-C5 antibody significantly reduced the mucosal expression of mRNAs for tumor necrosis factor-α, interleukin-1β and interleukin-6. In conclusion, the complement system plays a role in the development of dextran sodium sulfate-induced experimental colitis.
authors: Department of Medicine, Shiga University of Medical Science, Seta Tsukinowa, Otsu 520-2192, Japan ; Department of Pediatrics, Osaka Medical College, 2-7 Daigaku-cho, Takatsuki 569-0801, Japan.
authors: Tomoki
authors: T
authors: Aomatsu
authors: Hirotsugu
authors: H
authors: Imaeda
authors: Kenichiro
authors: K
authors: Takahashi
authors: Takehide
authors: T
authors: Fujimoto
authors: Eiji
authors: E
authors: Kasumi
authors: Hiromitsu
authors: H
authors: Ban
authors: Shigeki
authors: S
authors: Bamba
authors: Atsushi
authors: A
authors: Yoden
authors: Hiroshi
authors: H
authors: Tamai
authors: Yoshihide
authors: Y
authors: Fujiyama
authors: Akira
authors: A
authors: Andoh
keywords: DAF; immunology; inflammatory bowel disease