title: Microbial transformation of ginsenoside Rb1 to compound K by Lactobacillus paralimentarius.
abstract: In this study, the major ginsenoside Rb1 was transformed into the more pharmacologically active minor compound K by food grade Lactobacillus paralimentarius LH4, which was isolated from kimchi, a traditional Korean fermented food. The enzymatic reaction was analyzed by TLC, HPLC, and NMR. Using the cell-free enzyme of Lactobacillus paralimentarius LH4 at optimal conditions for 30 °C at pH 6.0, 1.0 mg ml(-1) ginsenoside Rb1 was transformed into 0.52 mg ml(-1) compound K within 72 h, with a corresponding molar conversion yield of 88 %. The cell-free enzyme hydrolyzed the two glucose moieties attached to the C-3 position and the outer glucose moiety attached to the C-20 position of the ginsenoside Rb1. The cell-free enzyme hydrolyzed the ginsenoside Rb1 along the following pathway: ginsenoside Rb1 → gypenoside XVII and ginsenoside Rd → ginsenoside F2 → compound K. Our results indicate that Lactobacillus paralimentarius LH4 has the potential to be applied for the preparation of compound K in the food industry.
authors: Department of Oriental Medicinal Material and Processing, College of Life Science, and Ginseng Genetic Resource Bank, Kyung-Hee University, Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors: Lin-Hu
authors: LH
authors: Quan
authors: Yeon-Ju
authors: YJ
authors: Kim
authors: Guan Hao
authors: GH
authors: Li
authors: Kwang-Tea
authors: KT
authors: Choi
authors: Deok-Chun
authors: DC
authors: Yang
mesh_terms: D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005516:Food Microbiology; D036145:Ginsenosides / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007723:Korea; D007778:Lactobacillus / Q000302:isolation & purification / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013696:Temperature; D013997:Time Factors
chemical_list: D036145:Ginsenosides; C442759:ginsenoside Rb1; C112772:ginsenoside compound K