title: A solution 17O-NMR approach for observing an oxidized cysteine residue in Cu,Zn-superoxide dismutase.
abstract: Solution (17)O-NMR application to biological macromolecules is extremely limited. We describe here (17)O-NMR observation of the (17)O(2)-oxidized cysteine side chain of human Cu,Zn-superoxide dismutase in solution using selective (17)O(2) oxidation. (17)O-NMR with the aid of (17)O-labeling has wide potential to probe the environment and dynamics of oxidizable functionalities in proteins.
authors: Structural Glycobiology Team, RIKEN ASI, 2-1 Hirosawa, Wako-shi, 351-0198 Saitama, Japan.
authors: Shinya
authors: S
authors: Hanashima
authors: Noriko
authors: N
authors: Fujiwara
authors: Kana
authors: K
authors: Matsumoto
authors: Noriyuki
authors: N
authors: Iwasaki
authors: Guo-qing
authors: GQ
authors: Zheng
authors: Hidetaka
authors: H
authors: Torigoe
authors: Keiichiro
authors: K
authors: Suzuki
authors: Naoyuki
authors: N
authors: Taniguchi
authors: Yoshiki
authors: Y
authors: Yamaguchi
mesh_terms: D003545:Cysteine / Q000737:chemistry*; D019281:Dimerization; D006801:Humans; D007553:Isotope Labeling; D019906:Nuclear Magnetic Resonance, Biomolecular; D010084:Oxidation-Reduction; D010103:Oxygen Isotopes / Q000737:chemistry; D012996:Solutions / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D010103:Oxygen Isotopes; D012996:Solutions; D013482:Superoxide Dismutase; D003545:Cysteine