title: Development of monoclonal antibodies and quantitative sandwich enzyme linked immunosorbent assay for the characteristic sialoglycoprotein of edible bird's nest.
abstract: The article presents a sandwich enzyme linked immunosorbent assay (ELISA) for identification of edible bird's nest. The characteristic sialoglycoproteins were found by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and purified by liquid-phase isoelectric focusing (LIEF). According to the analysis, the molecular weight was 106-128 kDa and the isoelectric point was ≤pH 3.0. Two anti-characteristic sialoglycoprotein monoclonal antibodies were produced. The monoclonal antibodies were examined by western-blot assay. One of the monoclonal antibody was used as coating and the other as the enzyme-labeled antibody after being coupled to horseradish peroxidase (HRP). Based on the optimized ELISA condition, the method was established with IC(50) of 1.5 ng/mL, and low cross-reactivity with various fake materials (<0.01%). ELISA provided a suitable means for screening of a large number of samples. The coefficients of variation were between 2.9% and 5.8%.
authors: Shenzhen Academy of Metrology and Quality Inspection, Shenzhen, P. R. China.
authors: Shiwei
authors: S
authors: Zhang
authors: Xintian
authors: X
authors: Lai
authors: Xiaoqing
authors: X
authors: Liu
authors: Yun
authors: Y
authors: Li
authors: Bifang
authors: B
authors: Li
authors: Xiuli
authors: X
authors: Huang
authors: Qinlei
authors: Q
authors: Zhang
authors: Wei
authors: W
authors: Chen
authors: Lin
authors: L
authors: Lin
authors: Guowu
authors: G
authors: Yang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D030161:Avian Proteins / Q000276:immunology*; D001717:Birds; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006735:Horseradish Peroxidase / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D012463:Saliva / Q000737:chemistry; D012795:Sialoglycoproteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D030161:Avian Proteins; D012795:Sialoglycoproteins; D006735:Horseradish Peroxidase