title: A fluorescent and chemiluminescent difunctional mesoporous silica nanoparticle as a label for the ultrasensitive detection of cancer cells.
abstract: A new kind of ultrabright fluorescent and chemiluminescent difunctional mesoporous silica nanoparticle (FCMSN) is reported. A luminescent dye, Rhodamine 6G or tris(2,2'-bipyridyl)dichlororuthenium(II) hexahydrate (Rubpy), is doped inside nanochannels of a silica matrix. The hydrophobic groups in the silica matrix avoid the leakage of dye from open channels. The amines groups on the surface of the FCMSN improve the modification performance of the nanoparticle. Because the nanochannels are isolated by a network skeleton of silica, fluorescence quenching based on the inner filter effect of the fluorescent dyes immobilized in nanochannels is weakened effectively. The Quantum Yield of obtained 90 nm silica particles was about 61%. Compared with the fluorescent core-shell nanoparticle, the chemiluminescence reagents can freely enter the nanoparticles to react with fluorescent dyes to create chemiluminescence. The results show that the FCMSN are both fluorescent labels and chemiluminescent labels. In biological applications, the NaIO(4) oxidation method was proven to be superior to the glutaraldehyde method. The amount of amino could affect the specificity of the FCMSN. The fluorescence microscopy imaging demonstrated that the FCMSN is viable for biological applications.
authors: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
authors: Liang
authors: L
authors: Tao
authors: Chaojun
authors: C
authors: Song
authors: Yuanjie
authors: Y
authors: Sun
authors: Xiaohua
authors: X
authors: Li
authors: Yunyun
authors: Y
authors: Li
authors: Boquan
authors: B
authors: Jin
authors: Zhujun
authors: Z
authors: Zhang
authors: Kun
authors: K
authors: Yang
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis*; D049408:Luminescent Agents / Q000737:chemistry; D008163:Luminescent Measurements; D008856:Microscopy, Fluorescence / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D009942:Organometallic Compounds / Q000737:chemistry; D010084:Oxidation-Reduction; D016062:Porosity; D012235:Rhodamines / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D049408:Luminescent Agents; D009942:Organometallic Compounds; D012235:Rhodamines; C415768:tris(2,2'-bipyridyl)ruthenium(II); C026188:rhodamine 6G; D012822:Silicon Dioxide