title: Influence of the human MIF promoter polymorphism on hepatocellular carcinoma prognosis.
abstract: Hepatocellular carcinoma (HCC) is one of the most common worldwide malignancies. A relative complete diagnosis system for primary carcinoma of liver has already been established, but the surgical prognosis for HCC, which depends mainly on postoperative pathological classification and data of recurrence and metastasis, lacks valid experimental indicators. Macrophage migration inhibition factor (MIF) is related to many cancers; hence, the polymorphism of MIF genes may be associated with the surgical prognosis of HCC. The purpose of this study was to investigate the relationship between polymorphisms of MIF gene promoter 794CATT (MIF-794CATT) microsatellite repeats and HCC surgical prognosis and evaluate the contribution of polymorphism to the prognosis of hepatectomy. Sequencing was used to identify the MIF-794CATT of 241 patients who had been submitted to HCC surgery. These patients were classified into 2 groups: one with MIF-794CATT high-repetitive-sequence genotypes (7/x+8/x) and one with low-repetitive-sequence genotypes (5/5+5/6+6/6). Five indictors were analyzed: average survival times were compared using the t-test, and tumor-node-metastasis staging, recurrence and metastasis, differentiation grade, and survival rate were compared using the chi-square test. The (7/x+8/x) CATT group had 139 patients and the (5/5+5/6+6/6) CATT group had 102. Significant differences were found in the 5 factors (P = 0.000, 0.008, 0.002, 0.000, and 0.003, respectively). Patients with MIF-794CATT5-8 low-repetitive-sequence genotypes had better prognosis than those with high-repetitive-sequence genotypes. The polymorphism detection of MIF-794CATT microsatellite repeats is valuable for HCC surgical prognosis.
authors: Department of Hepatobiliary Surgery, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, China.
authors: T
authors: T
authors: Yuan
authors: C
authors: C
authors: Tang
authors: M
authors: M
authors: Chen
authors: S
authors: S
authors: Deng
authors: P
authors: P
authors: Chen
mesh_terms: D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000401:mortality / Q000556:secondary / Q000601:surgery; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000235:genetics*; D008113:Liver Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D008207:Lymphatic Metastasis; D008263:Macrophage Migration-Inhibitory Factors / Q000235:genetics*; D018895:Microsatellite Repeats; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000517:prevention & control; D011110:Polymorphism, Genetic; D011379:Prognosis; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D016019:Survival Analysis
chemical_list: D008263:Macrophage Migration-Inhibitory Factors; D019746:Intramolecular Oxidoreductases; C515598:MIF protein, human