title: Clinical and prognostic significance of protocadherin-10 (PCDH10) promoter methylation in bladder cancer.
abstract: OBJECTIVE
  To investigate the clinical and prognostic significance of protocadherin-10 (PCDH10) promoter methylation in serum-derived DNA from patients with bladder cancer.
  
  
  METHODS
  PCDH10 promoter methylation status was determined using methylation-specific polymerase chain reaction of DNA extracted from serum of patients with bladder cancer, and age- and sex-matched controls. Clinical and pathological details of bladder cancer were recorded.
  
  
  RESULTS
  PCDH10 promoter methylation was detected in 59/117 (50.4%) of patients with bladder cancer, and none of 37 (0%) controls. Methylation was significantly associated with advanced stage (T(2)-T(4)), high grade (G(3)), tumour recurrence and larger tumour size (> 3 cm). In addition, methylation was associated with significantly worse survival and was an independent predictor of overall survival.
  
  
  CONCLUSIONS
  Serum-based analysis of PCDH10 promoter methylation may represent a useful noninvasive biomarker of malignant behaviour and outcome in bladder cancer.
authors: Department of Urology, Xuzhou Tumour Hospital, Xuzhou, Jiangsu Province, China.
authors: Y L
authors: YL
authors: Lin
authors: Z G
authors: ZG
authors: Li
authors: Z K
authors: ZK
authors: He
authors: T Y
authors: TY
authors: Guan
authors: J G
authors: JG
authors: Ma
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor; D015820:Cadherins / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000097:blood / Q000235:genetics; D019175:DNA Methylation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics; D009367:Neoplasm Staging; D011379:Prognosis; D011401:Promoter Regions, Genetic; D000091363:Protocadherins; D016019:Survival Analysis; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality*
chemical_list: D014408:Biomarkers, Tumor; D015820:Cadherins; C438603:PCDH10 protein, human; D000091363:Protocadherins; D004247:DNA