title: Dendritic cells administered intrarectally penetrate the intestinal barrier to break intestinal tolerance via Th2-medeiated colitis in mice.
abstract: Intestinal lamina propria dendritic cells (LPDCs) in mice are known to extend dendrites between the intestinal epithelia and the luminal side when processing luminal antigens. We conducted intrarectal cell transfer experiments of bone marrow-derived dendritic cells (BMDCs) in mice to assess dendritic cell penetration of the intestine. Intrarectally administered GFP(+) BMDCs localized in the colonic LP within 3h and the spleen within 12h after administration. 72h after administration, recipient C57BL/6 mice showed acute diarrhea, and administration of BMDCs (once weekly for 3 weeks) induced intestinal inflammation with increased numbers of recipient macrophages and CD4(+) T cells exhibiting a Th2-mediated immune response. These results demonstrate that DCs actively communicate across the intestinal barrier, and highlight a potential technique for controlling colonic immune tolerance.
authors: Department of Gastroenterology and Hepatology, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors: Tango
authors: T
authors: Handa
authors: Takanori
authors: T
authors: Kanai
authors: Toshiro
authors: T
authors: Sato
authors: Yohei
authors: Y
authors: Mikami
authors: Tomohisa
authors: T
authors: Sujino
authors: Atsushi
authors: A
authors: Hayashi
authors: Shinta
authors: S
authors: Mizuno
authors: Atsuhiro
authors: A
authors: Matsumoto
authors: Tadakazu
authors: T
authors: Hisamatsu
authors: Toshifumi
authors: T
authors: Hibi
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D039481:CD11b Antigen / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D003092:Colitis / Q000276:immunology*; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D004195:Disease Models, Animal; D007108:Immune Tolerance; D007413:Intestinal Mucosa / Q000276:immunology*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D051379:Mice; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D039481:CD11b Antigen