title: The production of 1,3-propanediol from mixtures of glycerol and glucose by a Klebsiella pneumoniae mutant deficient in carbon catabolite repression.
abstract: In the present study, mutant strain of Klebsiella pneumoniae with deletion of the crr gene encoding EIIA(Glc) (a component of the glucose-specific phosphoenolpyruvate-dependent transferase system [PTS]) was prepared. This eliminated the ability of the strain to mediate carbon catabolite repression (CCR). Production of 1,3-propanediol (1,3-PD) from glycerol by the crr mutant strain was enhanced (compared to that of the parent) in the presence of glucose. Using molasses as a co-substrate of glycerol, the maximum yield of 1,3-PD was 60.4% greater (81.2g/l) than that obtained when glycerol was used alone, under optimum fermentation conditions.
authors: Applied Microbiology Research Center, Bio-Materials Research Institute, KRIBB, Jeongeup, Jeonbuk 580-185, South Korea.
authors: Baek-Rock
authors: BR
authors: Oh
authors: Won-Kyung
authors: WK
authors: Hong
authors: Sun-Yeon
authors: SY
authors: Heo
authors: Lian Hua
authors: LH
authors: Luo
authors: Akihiko
authors: A
authors: Kondo
authors: Jeong-Woo
authors: JW
authors: Seo
authors: Chul Ho
authors: CH
authors: Kim
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D057465:Catabolite Repression / Q000235:genetics*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000378:metabolism*; D008965:Molasses; D009154:Mutation; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics; D011409:Propylene Glycols / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D011409:Propylene Glycols; C041787:1,3-propanediol; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D005947:Glucose; D005990:Glycerol