title: Cardiac progenitor-derived exosomes protect ischemic myocardium from acute ischemia/reperfusion injury.
abstract: BACKGROUND
  Cardiac progenitors (CPC) mediate cardioprotection via paracrine effects. To date, most of studies focused on secreted paracrine proteins. Here we investigated the CPC-derived-exosomes on protecting myocardium from acute ischemia/reperfusion (MI/R) injury.
  
  
  RESULTS
  CPC were isolated from mouse heart using two-step protocol. Exosomes were purified from conditional medium, and confirmed by electron micrograph and Western blot using CD63 as a marker. qRT-PCR shows that CPC-exosomes have high level expression of GATA4-responsive-miR-451. Exosomes were ex vivo labeled with PKH26, We observed exosomes can be uptaken by H9C2 cardiomyoblasts with high efficiency after 12 h incubation. CPC-exosomes protect H9C2 from oxidative stress by inhibiting caspase 3/7 activation invitro. In vivo delivery of CPC-exosomes in an acute mouse myocardial ischemia/reperfusion model inhibited cardiomyocyte apoptosis by about 53% in comparison with PBS control (p<0.05).
  
  
  CONCLUSIONS
  Our results suggest, for the first time, the CPC-exosomes can be used as a therapeutic vehicle for cardioprotection, and highlights a new perspective for using non-cell exosomes for cardiac disease.
authors: Department of Cardiology, Zhongda Hospital, Medical School of Southeast University, Nanjing 210009, China.
authors: Lijuan
authors: L
authors: Chen
authors: Yingjie
authors: Y
authors: Wang
authors: Yaohua
authors: Y
authors: Pan
authors: Lan
authors: L
authors: Zhang
authors: Chengxing
authors: C
authors: Shen
authors: Gangjian
authors: G
authors: Qin
authors: Muhammad
authors: M
authors: Ashraf
authors: Neal
authors: N
authors: Weintraub
authors: Genshan
authors: G
authors: Ma
authors: Yaoliang
authors: Y
authors: Tang
mesh_terms: D000818:Animals; D017209:Apoptosis; D004195:Disease Models, Animal; D055354:Exosomes; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D032386:Myoblasts, Cardiac / Q000378:metabolism* / Q000648:ultrastructure*; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D009206:Myocardium / Q000166:cytology*; D032383:Myocytes, Cardiac / Q000378:metabolism; D018384:Oxidative Stress; D011092:Polyethylene Glycols / Q000737:chemistry
chemical_list: D011092:Polyethylene Glycols