title: [Fast measurement method based on near infrared spectroscopy in purifying process of Carthamus tinctorius extracts].
abstract: OBJECTIVE
  To really realize quality control of Chinese herb purifying process, near-infrared spectroscopy (NIRS) was used not only for fast monitoring quality-control index of the process, but also for fast judgment of absorption endpoint.
  
  
  METHODS
  The purification process of Carthamus tinctorius extracts with nonionic macroreticular resin was selected as an example. HPLC was used as the reference method to determine the content of HSYA. Quantitative and qualitative detection modes of purification were developed by NIRS combined with partial least squares (PLS) and moving block of standard deviation (MBSD).
  
  
  RESULTS
  The correlation coefficient of the calibration model was 0.999, and the RPD for calibration and validation were above 5, of 5.54 and 5.22, respectively. Based on acquisition spectra, absorption endpoint calculated by MBSD was close to that by HPLC, Only 1 min deviation.
  
  
  CONCLUSIONS
  The method mentioned above is proved to be convenient, rapid and nondestructive, and is applicable for fast monitoring the content of HSYA and fast judgment of absorption endpoint in purifying process of C. tinctorius extracts.
authors: Hangzhou Binjiang Hospital, Hangzhou 310052, China.
authors: Xue-Ying
authors: XY
authors: Chen
authors: Xiang
authors: X
authors: Xu
authors: Yong
authors: Y
authors: Chen
authors: Xue-Song
authors: XS
authors: Liu
mesh_terms: D031187:Carthamus tinctorius / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000302:isolation & purification; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal