title: High density DNA loading on the M13 bacteriophage provides access to colorimetric and fluorescent protein microarray biosensors.
abstract: We report the synthesis, characterization, and protein sensing capabilities of M13 bacteriophage-DNA bioconjugates. DNA oligonucleotides were conjugated to M13 through acyl hydrazone linkages. In one case, DNAzymes retained their catalytic ability when anchored to the virus coat, and in a separate study, the dynamic nature of the hydrazone allowed for liberation of DNA from the phage under mild conditions.
authors: Department of Chemical and Biological Engineering, University of Colorado, Boulder, Boulder, CO, USA.
authors: Dylan W
authors: DW
authors: Domaille
authors: Ju Hun
authors: JH
authors: Lee
authors: Jennifer N
authors: JN
authors: Cha
mesh_terms: D015374:Biosensing Techniques; D003124:Colorimetry; D021881:DNA, Catalytic / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D006835:Hydrazones / Q000737:chemistry; D007074:Immunoglobulin G / Q000276:immunology; D009841:Oligonucleotides / Q000737:chemistry*; D019151:Peptide Library; D040081:Protein Array Analysis; D011506:Proteins / Q000032:analysis*
chemical_list: D021881:DNA, Catalytic; D005456:Fluorescent Dyes; D006835:Hydrazones; D007074:Immunoglobulin G; D009841:Oligonucleotides; D019151:Peptide Library; D011506:Proteins