title: rRNA (rrn) operon-engineered Bacillus subtilis as a feasible test organism for antibiotic discovery.
abstract: Bacillus subtilis contains 10 rRNA (rrn) operons. We found that rRNA operon-engineered B. subtilis strain RIK543, with only the rrnO operon, is specifically hypersensitive to RNA polymerase inhibitors such as rifamycin SV and rifampin (80-fold and 20-fold, respectively). In pilot screening experiments, we found actinomycete isolates successfully at an incidence of 1.9% (18/945) that produced antibacterials that were detectable only with RIK543 as the test organism. Strain RIK543 may be a feasible test organism for the discovery of novel RNA polymerase inhibitors.
authors: Department of Life Science, Hiroshima Institute of Technology, Saeki-ku, Hiroshima, Japan.
authors: Yukinori
authors: Y
authors: Tanaka
authors: Hideaki
authors: H
authors: Nanamiya
authors: Koichi
authors: K
authors: Yano
authors: Koji
authors: K
authors: Kakugawa
authors: Fujio
authors: F
authors: Kawamura
authors: Kozo
authors: K
authors: Ochi
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects* / Q000235:genetics*; D012407:rRNA Operon / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents