title: Differential protein expression profile between CD20 positive and negative cells of the NCI-H929 cell line.
abstract: At present, multiple myeloma (MM) remains an incurable disease and cologenic cells may be responsible for disease relapse. It has been proposed that CD20+/CD138- NCI-H929 cells could be hallmarks of MM clonogenic cells. Here, the immunology phenotype of NCI-H929 cells is described. Only a small population of CD20+/CD138- cells (<1%) was found in the NCI-H929 cell line, but CD20+/CD138- cells were not detected. We found that CD20+/CD138+ cells were able to exhibit cologenic capacity by colony formation assay and continuous passage culture. Proteins were analyzed by 1D-SDS-PAGE and TMT based quantitative differential liquid chromatography tandem mass spectrometry (LC-MS/MS). 1,082 non-redundant proteins were identified, 658 of which were differentially expressed with at least a 1.5-fold difference. 205 proteins in CD20+ cells were expressed at higher levels and 453 proteins were at lower levels compared with CD20- cells. Most proteins had catalytic and binding activity and mainly participated in metabolic processes, cell communication and molecular transport. These results proved that there are different biological features and protein expression profile between CD20+ and CD20- cells in the NCI-H929 cell line.
authors: Department of Hematology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.
authors: Chuan-Ying
authors: CY
authors: Geng
authors: Nian
authors: N
authors: Liu
authors: Guang-Zhong
authors: GZ
authors: Yang
authors: Ai-Jun
authors: AJ
authors: Liu
authors: Yun
authors: Y
authors: Leng
authors: Hui-Juan
authors: HJ
authors: Wang
authors: Li-Hong
authors: LH
authors: Li
authors: Yin
authors: Y
authors: Wu
authors: Yan-Chen
authors: YC
authors: Li
authors: Wen-Ming
authors: WM
authors: Chen
mesh_terms: D018951:Antigens, CD20 / Q000378:metabolism*; D002853:Chromatography, Liquid; D003114:Colony-Forming Units Assay; D019295:Computational Biology; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D006801:Humans; D009101:Multiple Myeloma / Q000175:diagnosis / Q000378:metabolism*; D054547:National Cancer Institute (U.S.); D009363:Neoplasm Proteins / Q000032:analysis*; D053668:Syndecan-1 / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D014407:Tumor Cells, Cultured; D014481:United States
chemical_list: D018951:Antigens, CD20; D009363:Neoplasm Proteins; D053668:Syndecan-1