title: Variations of CHROMagar Acinetobacter to detect imipenem-resistant Acinetobacter baumannii-calcoaceticus complex.
abstract: BACKGROUND
  Acinetobacter baumannii-calcoaceticus complex (ABC) isolates are often multidrug-resistant, including to carbapenems. Chromogenic media can facilitate the rapid detection of Gram-negative bacteria, often with the addition of supplements to a base chromogenic medium to detect resistance. We examined various combinations of available media to detect imipenem resistance among 107 ABC clinical isolates.
  
  
  METHODS
  CHROMagar Orientation, CHROMagar KPC, and CHROMagar Acinetobacter, by itself, with Acinetobacter supplement, with KPC supplement, or CHROMagar Acinetobacter with increasing concentrations (1, 2.5, and 5 ml/l) of a new CR102 supplement, were examined.
  
  
  RESULTS
  Sensitivity for the detection of isolates was high (> 98%) for all formulations. Specificity was high for CHROMagar Acinetobacter with 2.5 ml/l and 5 ml/l of the CR102 supplement, at 95.3% and 97.7%, respectively, with positive predictive values of 97% and 98.5%. Negative predictive values of these 2 formulations were 100%.
  
  
  CONCLUSIONS
  CHROMagar Acinetobacter with the addition of the CR102 supplement at 2.5 ml/l and 5ml/l is highly sensitive and specific for the detection of imipenem-resistant ABC, and may be useful for the rapid detection of imipenem-resistant ABC in clinical samples.
authors: Infectious Disease Service, San Antonio Military Medical Center, Fort Sam Houston, Texas, USA.
authors: Alice
authors: A
authors: Barsoumian
authors: Tatjana
authors: T
authors: Calvano
authors: Ana E
authors: AE
authors: Markelz
authors: Ryan
authors: R
authors: Cassidy
authors: Clinton K
authors: CK
authors: Murray
authors: Miriam L
authors: ML
authors: Beckius
authors: Katrin
authors: K
authors: Mende
authors: Kevin S
authors: KS
authors: Akers
mesh_terms: D000151:Acinetobacter Infections / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification; D016954:Acinetobacter calcoaceticus / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015373:Bacterial Typing Techniques / Q000379:methods*; D003470:Culture Media; D006801:Humans; D015378:Imipenem / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D011237:Predictive Value of Tests; D018440:beta-Lactam Resistance
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D015378:Imipenem