title: Suppression of AcMNPV replication by adf and thymosin protein up-regulation in a new testis cell line, Ha-shl-t.
abstract: Host cytoskeletons facilitate the entry, replication, and egress of viruses because cytoskeletons are essential for viral survival. One mechanism of resisting viral infections involves regulating cytoskeletal polymerization/depolymerization. However, the molecular mechanisms of regulating these changes in cytoskeleton to suppress viral replication remain unclear. We established a cell line (named Ha-shl-t) from the pupal testis of Helicoverpa armigera (Lepidoptera: Noctuidae). The new testis cell line suppresses Autographa californica multiple nucleocapsid nucleopolyhedrovirus (AcMNPV) replication via disassembly of cytoskeleton. Up-regulation of thymosin (actin disassembling factor) and adf (actin depolymerizing factor) reduces F-actin. Silencing thymosin or adf or treating cells with the F-actin stabilizer phalloidin led to increased AcMNPV replication, while treating cells with an F-actin assembly inhibitor cytochalasin B decreased viral replication. We infer that Ha-shl-t cells utilize F-actin depolymerization to suppress AcMNPV replication by up-regulating thymosin and adf. We propose Ha-shl-t as a model system for investigating cytoskeletal regulation in antiviral action and testicular biology generally.
authors: Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan, China.
authors: Xiaoqian
authors: X
authors: Zhang
authors: Ming
authors: M
authors: Chen
authors: Xinlei
authors: X
authors: Ma
authors: Xiaofan
authors: X
authors: Zhao
authors: Jinxing
authors: J
authors: Wang
authors: Honglian
authors: H
authors: Shao
authors: Qisheng
authors: Q
authors: Song
authors: David
authors: D
authors: Stanley
mesh_terms: D051339:Actin Depolymerizing Factors / Q000378:metabolism*; D000199:Actins / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D003599:Cytoskeleton / Q000378:metabolism; D054884:Host-Pathogen Interactions; D008297:Male; D009036:Moths / Q000502:physiology / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000502:physiology*; D013737:Testis / Q000166:cytology; D013947:Thymosin / Q000378:metabolism*; D015854:Up-Regulation; D019562:Viral Load; D014779:Virus Replication
chemical_list: D051339:Actin Depolymerizing Factors; D000199:Actins; D013947:Thymosin