title: Identifying source proteins for MHC class I-presented peptides.
abstract: Identification of antigenic peptides recognized by cytolytic T lymphocytes (CTL) is a prerequisite for the development of targeted cancer immunotherapy approaches. This chapter provides a global approach for the identification of peptides recognized by CTL. It implies the identification of the HLA molecule presenting the peptide as well as the design and screening of a cDNA library derived from the tumor cells.
authors: Ludwig Institute for Cancer Research, Brussels Branch and WELBIO and de Duve Institute, Université catholique de Louvain, Brussels, Belgium.
authors: Nathalie
authors: N
authors: Vigneron
authors: Ludwig Institute for Cancer Research, Brussels Branch and WELBIO and de Duve Institute, Université catholique de Louvain, Brussels, Belgium.
authors: Wenbin
authors: W
authors: Ma
authors: Ludwig Institute for Cancer Research, Brussels Branch and WELBIO and de Duve Institute, Université catholique de Louvain, Brussels, Belgium.
authors: Alexandre
authors: A
authors: Michaux
authors: Ludwig Institute for Cancer Research, Brussels Branch and WELBIO and de Duve Institute, Université catholique de Louvain, Brussels, Belgium. benoit.vandeneynde@bru.licr.org.
authors: Benoît J
authors: BJ
authors: Van den Eynde
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D019556:COS Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D057809:HEK293 Cells; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006801:Humans; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D010766:Phosphorylation; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014162:Transfection; D014170:Transformation, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015395:Histocompatibility Antigens Class I; D010446:Peptide Fragments; D014409:Tumor Necrosis Factor-alpha