title: Lansiumamide B and SB-204900 isolated from Clausena lansium inhibit histamine and TNF-α release from RBL-2H3 cells.
abstract: OBJECTIVE
  Mast cells play a central role in allergic and chronic inflammation. Extracts from Clausena lansium (Lour.) Skeels (Rutaceae) possess many pharmacological effects including anti-inflammatory, anti-oxidant, anti-cancer, and anti-trichomonal activities. In addition, the leaves and fruit are used in Chinese folk medicine. We have isolated and identified four known cinnamamides from this plant: lansiumamide C, lansamide I, lansiumamide B, and SB-204900. However, the biological activities of these compounds are not yet understood. The purpose of this paper is to clarify the pharmacological effects of these compounds on mast cells.
  
  
  METHODS
  We measured inflammatory molecules in A23187-stimulated rat basophilic leukemia cells (RBL-2H3) treated with these compounds using HPLC, ELISA, and immunoblotting methods. In addition, some signaling molecules were investigated by immunoblotting.
  
  
  RESULTS
  Lansamide I, lansiumamide B, and SB-204900 significantly decreased histamine release. Furthermore, lansiumamide B- and SB-204900-treated cells also reduced the protein and/or mRNA levels of TNF-α. SB-204900 markedly suppressed the phosphorylation of p38 MAPK.
  
  
  CONCLUSIONS
  Our findings suggest that lansiumamide B and SB-204900 attenuate mast-cell-induced inflammation.
authors: Faculty of Pharmacy, Meijo University, Tempaku-ku, Nagoya, Aichi, Japan. tmatsui@aichi-med-u.ac.jp
authors: Takuya
authors: T
authors: Matsui
authors: Chihiro
authors: C
authors: Ito
authors: Hiroshi
authors: H
authors: Furukawa
authors: Tadashi
authors: T
authors: Okada
authors: Masataka
authors: M
authors: Itoigawa
mesh_terms: D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D061207:Calcium Ionophores / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002934:Cinnamates / Q000494:pharmacology*; D054700:Clausena; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004195:Disease Models, Animal; D006632:Histamine / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism; D015471:Leukemia, Basophilic, Acute / Q000378:metabolism* / Q000473:pathology; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D061207:Calcium Ionophores; D002934:Cinnamates; D015850:Interleukin-6; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D000001:Calcimycin; D006632:Histamine; D051546:Cyclooxygenase 2; D048051:p38 Mitogen-Activated Protein Kinases; C411088:cinnamamide