title: The comparative immunotoxicity of mesoporous silica nanoparticles and colloidal silica nanoparticles in mice.
abstract: BACKGROUND
  Mesoporous silica (MPS) nanoparticles (NPs), which have a unique pore structure and extremely large surface area and pore volume, have received much attention because of their biomedical application potential. Using MPS NPs for biomedical devices requires the verification of their biocompatibility because the surface area of NPs is one of the most important determinants of toxicity, including the cellular uptake and immune response. We have previously reported that the cytotoxicity and inflammation potential of MPS NPs have been shown to be lower than those of general amorphous colloidal silica (Col) NPs in macrophages, but the low cytotoxicity does not guarantee high biocompatibility in vivo. In this study, we compared the in vivo immunotoxicity of MPS and Col NPs in the mouse model to define the effects of pore structural conditions of silica NPs.
  
  
  METHODS
  Both MPS and Col NPs (2, 20, and 50 mg/kg/day) were intraperitoneally administered in female BALB/c mice for 4 weeks, and clinical toxicity, lymphocyte population, serum IgG/IgM levels, and histological changes were examined.
  
  
  RESULTS
  There was no overt sign of clinical toxicity in either MPS- or Col-treated mice. However, MPS NPs led to significant increases in liver and spleen weight and splenocyte proliferation. Mice treated with MPS NPs showed altered lymphocyte populations (CD3(+), CD45(+), CD4(+), and CD8(+)) in the spleen, increased serum IgG and IgM levels, and histological changes. Despite slight changes in lymphocyte populations in the spleen, Col NPs did not alter other immunological factors.
  
  
  CONCLUSIONS
  The results indicate that in vivo exposure to MPS NPs caused more damage to systemic immunity than that of Col NPs through the dysregulation of the spleen. The results for in vivo data are inconsistent with those for in vitro data, which show lower cytotoxicity for MPS NPs. These results suggest the importance of verifying biocompatibility both in vitro and in vivo during the design of new nanomaterials.
authors: CMRI, Laboratory of Immunotoxicology, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors: Soyoung
authors: S
authors: Lee
authors: Mi-Sun
authors: MS
authors: Kim
authors: Dakeun
authors: D
authors: Lee
authors: Taeg Kyu
authors: TK
authors: Kwon
authors: Dongwoo
authors: D
authors: Khang
authors: Hui-Suk
authors: HS
authors: Yun
authors: Sang-Hyun
authors: SH
authors: Kim
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003102:Colloids / Q000008:administration & dosage / Q000633:toxicity*; D004326:Drinking / Q000187:drug effects; D004435:Eating / Q000187:drug effects; D005260:Female; D006651:Histocytochemistry; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007263:Infusions, Parenteral; D008214:Lymphocytes / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage / Q000633:toxicity*; D012822:Silicon Dioxide / Q000008:administration & dosage / Q000276:immunology / Q000633:toxicity*; D013154:Spleen / Q000737:chemistry / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D003102:Colloids; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012822:Silicon Dioxide
keywords: colloidal silica nanoparticle; immunotoxicity; mesoporous silica nanoparticle; spleen