title: Separation and fractionation of exopolysaccharides from Porphyridium cruentum.
abstract: In this work the extraction of EPSs from culture media of Porphyridium cruentum, by dialysis, solvent-precipitation with 3 polar alcohols (methanol, ethanol and isopropanol) and membrane separation techniques has been studied. Diafiltration (DF) using a membrane with a 300 kDa molecular weight cut off was the most efficient technique compared to solvent-extraction and dialysis methods. After extraction, EPS fraction was characterized in terms of rheological properties and biochemical content. The product exhibited shear thinning behavior and a critical overlap concentration equal to 0.6 g/L. The monosaccharide composition was investigated after acidic hydrolysis. Xylose, galactose, glucose and glucuronic acid were identified as the main constitutive monomers.
authors: Clermont Université, Université Blaise Pascal, Institut Pascal UMR CNRS 6602, Aubière, France.
authors: Anil Kumar
authors: AK
authors: Patel
authors: Celine
authors: C
authors: Laroche
authors: Alain
authors: A
authors: Marcati
authors: Alina Violeta
authors: AV
authors: Ursu
authors: Sébastien
authors: S
authors: Jubeau
authors: Luc
authors: L
authors: Marchal
authors: Emmanuel
authors: E
authors: Petit
authors: Gholamreza
authors: G
authors: Djelveh
authors: Philippe
authors: P
authors: Michaud
mesh_terms: D019840:2-Propanol; D011232:Chemical Precipitation; D003470:Culture Media / Q000737:chemistry; D003956:Dialysis / Q000379:methods; D000431:Ethanol; D005374:Filtration / Q000379:methods; D006868:Hydrolysis; D000432:Methanol; D009005:Monosaccharides / Q000032:analysis*; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification*; D044665:Porphyridium / Q000737:chemistry*; D012212:Rheology
chemical_list: D003470:Culture Media; D009005:Monosaccharides; D011135:Polysaccharides, Bacterial; D000431:Ethanol; D019840:2-Propanol; D000432:Methanol
keywords: Diafiltration; Exopolysaccharide; Porphyridium sp.