title: Screening and analyzing the potential bioactive components from reduning injection, using macrophage cell extraction and ultra-high performance liquid chromatography coupled with mass spectrometry.
abstract: In this paper, a useful method for screening and analyzing the potential bioactive components from Reduning Injection was developed using macrophage cell extraction and ultra-high performance liquid chromatography coupled with Q-TOF/MS spectrometry. In addition, the protective effects on macrophage cell damage induced by LPS in vitro were also investigated. The results showed that chlorogenic acid, 3, 4-dicaffeoylquinic acid, 3, 5-dicaffeoylquinic acid and 4, 5-dicaffeoylquinic acid significantly inhibited the prostaglandin E(2) (PGE(2)) release and reversed the interleukin-6 (IL-6) secretion of macrophages (p < 0.05). These data indicated that the method of macrophage cell extraction coupled with UPLC-MS technology is feasible, rapid and useful for screening and analyzing potential bioactive components from TCM injection.
authors: State Key Lab of New-Tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, P. R. China.
authors: Yonghui
authors: Y
authors: Li
authors: Peixiang
authors: P
authors: Wang
authors: Wei
authors: W
authors: Xiao
authors: Li
authors: L
authors: Zhao
authors: Zhenzhong
authors: Z
authors: Wang
authors: Li
authors: L
authors: Yu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents; D002478:Cells, Cultured; D002726:Chlorogenic Acid / Q000031:analogs & derivatives* / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015232:Dinoprostone / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D018836:Inflammation Mediators / Q000378:metabolism*; D007267:Injections; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000009:adverse effects; D008264:Macrophages / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice
chemical_list: D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D018836:Inflammation Mediators; D015850:Interleukin-6; D008070:Lipopolysaccharides; D002726:Chlorogenic Acid; D015232:Dinoprostone