title: Isolation of a substantial proportion of forest soil bacterial communities detected via pyrotag sequencing.
abstract: We isolated 1,264 bacterial strains from forest soils previously surveyed via pyrosequencing of rRNA gene amplicons. Conventional culturing techniques recovered a substantial proportion of the community, with isolates representing 22% of 98,557 total pyrotags. Growth characteristics of isolates indicated that ecological traits were associated with relative abundances of corresponding pyrotag operational taxonomic units.
authors: Department of Microbiology & Immunology, Life Sciences Institute, University of British Columbia, Vancouver, British Columbia, Canada.
authors: David
authors: D
authors: VanInsberghe
authors: Martin
authors: M
authors: Hartmann
authors: Gordon R
authors: GR
authors: Stewart
authors: William W
authors: WW
authors: Mohn
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods*; D012988:Soil Microbiology; D014197:Trees
chemical_list: D004269:DNA, Bacterial