title: High throughput phenotypic selection of Mycobacterium tuberculosis mutants with impaired resistance to reactive oxygen species identifies genes important for intracellular growth.
abstract: Mycobacterium tuberculosis has the remarkable capacity to survive within the hostile environment of the macrophage, and to resist potent antibacterial molecules such as reactive oxygen species (ROS). Thus, understanding mycobacterial resistance mechanisms against ROS may contribute to the development of new anti-tuberculosis therapies. Here we identified genes involved in such mechanisms by screening a high-density transposon mutant library, and we show that several of them are involved in the intracellular lifestyle of the pathogen. Many of these genes were found to play a part in cell envelope functions, further strengthening the important role of the mycobacterial cell envelope in protection against aggressions such as the ones caused by ROS inside host cells.
authors: Unité de Génétique Mycobactérienne, Institut Pasteur, Paris, France. olga.mestre@pasteur.fr
authors: Olga
authors: O
authors: Mestre
authors: Raquel
authors: R
authors: Hurtado-Ortiz
authors: Tiago
authors: T
authors: Dos Vultos
authors: Amine
authors: A
authors: Namouchi
authors: Mena
authors: M
authors: Cimino
authors: Madalena
authors: M
authors: Pimentel
authors: Olivier
authors: O
authors: Neyrolles
authors: Brigitte
authors: B
authors: Gicquel
mesh_terms: D060467:Disease Resistance / Q000235:genetics; D005798:Genes, Bacterial / Q000502:physiology*; D057166:High-Throughput Screening Assays; D054884:Host-Pathogen Interactions / Q000235:genetics; D006801:Humans; D008264:Macrophages / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*; D010641:Phenotype; D017382:Reactive Oxygen Species / Q000378:metabolism / Q000494:pharmacology*; D014397:Tuberculosis, Pulmonary / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control
chemical_list: D017382:Reactive Oxygen Species