title: Stop-flow comprehensive two-dimensional liquid chromatography combined with mass spectrometric detection for phospholipid analysis.
abstract: A novel comprehensive two-dimensional liquid chromatographic (LC × LC) system for characterization of phospholipid (PL) molecular species belonging to six phospholipid classes was developed. To tackle such a task, a silica hydrophilic interaction liquid chromatography (HILIC) column was used as the first dimension ((1)D), and reversed-phase liquid chromatography (RP-LC) with a C18 column was used as the second dimension ((2)D) in combination with mass spectrometric detection. Fraction transfer from the (1)D to the (2)D was performed by means of a two-position ten-port switching valve, operated under stop-flow conditions. The capability of the investigated LC × LC approach was demonstrated in the separation of phospholipid molecular species contained in two Folch-extracted cow's milk and plasma samples. Within the phosphatidylcholine class, up to 16 and 14 different species were identified, respectively, by means of the HILIC×RP-LC-MS system employed under both positive and negative ionization mode.
authors: Dipartimento di Scienze del Farmaco e dei Prodotti della Salute, University of Messina, viale Annunziata, 98168 Messina, Italy. pdugo@unime.it
authors: Paola
authors: P
authors: Dugo
authors: Nermeen
authors: N
authors: Fawzy
authors: Filomena
authors: F
authors: Cichello
authors: Francesco
authors: F
authors: Cacciola
authors: Paola
authors: P
authors: Donato
authors: Luigi
authors: L
authors: Mondello
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D010743:Phospholipids / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D010743:Phospholipids