title: Osteoclast-inhibiting saikosaponin derivatives from Bupleurum chinense.
abstract: Five new saikosaponins, saikosaponin w (1), 21β-hydroxysaikosaponin b2 (2), 6″-O-acetylsaikosaponin e (3), 6″-O-acetylsaikosaponin b1 (4), and 6″-O-acetylsaikosaponin b3 (5), along with twenty-two known ones (6-27), have been isolated from the roots of Bupleurum chinense. Their structures were elucidated on the basis of detailed spectroscopic analysis, including mainly 1D and 2D NMR and HRESI-MS, qualitative chemical methods, and comparison with the literatures. Osteoclast-inhibiting activity of some of the isolated compounds was evaluated in vitro, with five ones have shown significant activity by inhibitory rates ranging from 48.3% to 56.1% at the concentration of 10μM and with significant differences among groups observed.
authors: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China. fish@imm.ac.cn
authors: Jin-Qian
authors: JQ
authors: Yu
authors: An-Jun
authors: AJ
authors: Deng
authors: Lian-Qiu
authors: LQ
authors: Wu
authors: Zhi-Hui
authors: ZH
authors: Zhang
authors: Yang
authors: Y
authors: Liu
authors: Wen-Jie
authors: WJ
authors: Wang
authors: Hai-Lin
authors: HL
authors: Qin
mesh_terms: D000818:Animals; D029972:Bupleurum / Q000737:chemistry*; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical; D051379:Mice; D008810:Mice, Inbred C57BL; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010010:Osteoclasts / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: C051274:Bupleurum root extract; D010936:Plant Extracts; D012503:Saponins; D009828:Oleanolic Acid; C025759:saikosaponin D