title: Fuel cell virus sensor using virus capture within antibody-coated nanochannels.
abstract: We report a unique fuel cell sensor system for the first time direct detection of unlabeled virus particles based on the formation of antibody-virus complexes within the sensor's membrane nanochannels. This strategy exploits the change in the membrane resistance of the powered system, comprising a Prussian blue nanotubes (PB-nt) membrane cathode and a platinum mesh anode. The method reports an impressive shortest response time of ∼5 min toward the specific virus target, at low concentration values of 3-45 plaque-forming units per milliliter (pfu mL(-1)) with detection limit of 0.04 pfu mL(-1), comparable to state-of-the-art polymerase chain reaction (PCR)-based methods. The sensor can clearly differentiate dengue virus serotype 2 from serotype 3. When filled with Nafion perfluorinated resin, the PB-nt membrane demonstrates powerful utilization as a stand-alone fuel cell based virus sensor, and thus offers the outstanding promise of a sustainable, low-cost, and rapid low-power virus detection tool.
authors: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637371.
authors: Yanyan
authors: Y
authors: Wei
authors: Lai Peng
authors: LP
authors: Wong
authors: Chee-Seng
authors: CS
authors: Toh
mesh_terms: D000330:Aedes; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000378:metabolism*; D003716:Dengue Virus / Q000737:chemistry / Q000378:metabolism*; D005295:Ferrocyanides / Q000737:chemistry; D051379:Mice; D043942:Nanotubes / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D005295:Ferrocyanides; C000170:ferric ferrocyanide