title: A new strategy for the selective determination of glutathione in the presence of nicotinamide adenine dinucleotide (NADH) using a novel modified carbon nanotube paste electrode.
abstract: A novel electrochemical sensor for the simultaneous determination of glutathione (GSH) and nicotinamide adenine dinucleotide (NADH) is described. The sensor is based on a carbon paste electrode (CPE) modified with benzamide derivative and multiwall carbon nanotubes. This mixture makes a modified electrode that is sensitive for the electrochemical detection of these compounds. Under optimum conditions and at pH 7.0, oxidation of GSH occurs at a potential of about 330 mV less positive than that at an unmodified CPE. The voltammetric peak currents are linearly dependent on GSH and NADH concentrations in the ranges 0.09-300 μmol L(-1) GSH and 5.0-600 μmol L(-1) NADH. The detection limits found for GSH and NADH were 0.05 μmol L(-1) and 1.0 μmol L(-1), respectively. The electrochemical sensor was also used for the determination of GSH in urine, pharmaceutical and hemolysed erythrocyte samples.
authors: Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran. Ensafi@cc.iut.ac
authors: A A
authors: AA
authors: Ensafi
authors: Hassan
authors: H
authors: Karimi-Maleh
authors: S
authors: S
authors: Mallakpour
mesh_terms: D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D005978:Glutathione / Q000032:analysis*; D009243:NAD / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D037742:Nanotubes, Carbon; D009243:NAD; D005978:Glutathione