title: Effect of hypoxia on neural induction in colonies of human parthenogenetic stem cells.
abstract: We studied neural induction and generation of neuroectoderm in the colonies of human parthenogenetic SC cultured in the presence of 5 and 19±2% oxygen. We found that neuroectoderm was more actively generated at high oxygen content. At the same time, the transcription of stem cell pluripotency genes was not completely suppressed during neural induction at low oxygen content, while the expression of endoderm and mesodermal marker genes attested to the absence of specific differentiation. These findings demonstrate more efficient neuroectoderm generation induced in the colonies of pluripotent stem cells under conditions of normoxia.
authors: International Stem Cell Corporation, Carlsbad, USA.
authors: T V
authors: TV
authors: Abramihina
authors: D A
authors: DA
authors: Isaev
authors: R A
authors: RA
authors: Semechkin
mesh_terms: D018797:Cell Cycle Proteins; D002454:Cell Differentiation; D015687:Cell Hypoxia; D002478:Cells, Cultured; D005136:Eye Proteins / Q000378:metabolism; D051858:Forkhead Transcription Factors; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D000071317:Nanog Homeobox Protein; D054258:Neural Plate / Q000166:cytology*; D055495:Neurogenesis; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors / Q000378:metabolism; D010312:Parthenogenesis; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D005136:Eye Proteins; C108803:FOXO4 protein, human; D051858:Forkhead Transcription Factors; D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; D000071841:PAX6 Transcription Factor; C000605730:PAX6 protein, human; C494173:POU5F1 protein, human; D051761:Paired Box Transcription Factors; D012097:Repressor Proteins; C490906:SOX1 protein, human; C527785:SOX3 protein, human; D055748:SOXB1 Transcription Factors; D014157:Transcription Factors