title: Receptor-mediated endocytosis and trafficking between endosomal-lysosomal vacuoles in Giardia lamblia.
abstract: The early branching Giardia lamblia has highly polarized vacuoles, located underneath the plasma membrane, which have at least some of the characteristics of endosomes and of lysosomes. These peripheral vacuoles (PVs) are necessary for nutrient uptake and the maintenance of plasma membrane composition, but whether they carry out sorting and segregation of receptors and ligands is a matter of debate. Here, we showed that the internalization of low-density lipoprotein (LDL) to the PVs is highly dynamic in trophozoites with a rate similar to the internalization of the low-density lipoprotein receptor-related protein 1. Moreover, by analyzing receptor-mediated and fluid-phase endocytosis in living cells, we showed that after endocytosis LDL but not dextran moved laterally between the PVs. We speculate on PV functional heterogeneity and maturation in this parasite.
authors: Instituto de Investigación Médica Mercedes y Martín Ferreyra, INIMEC-CONICET, Universidad Nacional de Córdoba, Friuli 2434, 5000, Córdoba, Argentina.
authors: Maria R
authors: MR
authors: Rivero
authors: Ignacio
authors: I
authors: Jausoro
authors: Mariano
authors: M
authors: Bisbal
authors: Constanza
authors: C
authors: Feliziani
authors: Adriana
authors: A
authors: Lanfredi-Rangel
authors: Maria C
authors: MC
authors: Touz
mesh_terms: D003911:Dextrans / Q000378:metabolism; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism*; D016829:Giardia lamblia / Q000378:metabolism / Q000502:physiology*; D008077:Lipoproteins, LDL / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D003911:Dextrans; D008077:Lipoproteins, LDL