title: Effect of different extenders on in vitro characteristics of feline epididymal sperm during cryopreservation.
abstract: To evaluate and compare the efficacy of various extenders for the cryopreservation of epididymal cat spermatozoa, two experiments were planned. Bovine and equine commercial extenders in the experiment 1 and TRIS-egg yolk-based extenders in experiment 2 were separately studied since the number of sperm collected per cat is reduced. Epididymal sperm samples were packaged into 0.25-ml straws and frozen. Vigour, motility, morphology, acrosome status, sperm viability and functional membrane integrity were assessed at collection, after cooling and after thawing, while DNA integrity was evaluated at 0- and 6-h post-thaw. Experiment 1 compared the effect of three non-feline commercial extenders - based on TRIS-egg yolk (Triladyl), egg-yolk-free medium (AndroMed) and skimmed milk-egg yolk (Gent) - on the quality of frozen-thawed epididymal cat sperm. Values for sperm motility and functional membrane integrity in cooled sperm diluted in Triladyl were higher (p < 0.001) than those recorded for Andromed and Gent. Except sperm morphology, the other assessed characteristics showed significant higher values in frozen-thawed sperm diluted in Triladyl than in Andromed and Gent extenders. Experiment 2 analysed the effects of three TRIS-egg yolk-based extenders, one non-feline commercial (Triladyl) and the other two prepared using different monosaccharides (glucose and fructose), on freezing-thawed sperm. Results showed that specifically prepared extenders for cryopreservation of feline spermatozoa performed better than the commercial extender Triladyl, although sperm quality during the freezing-thawing process did not significantly differ associated with the type of monosaccharide (glucose vs fructose) added to the mentioned extenders. Although TRIS-egg yolk-based extenders prepared in experiment 2 improved sperm cryoprotection, Triladyl remains a good option for practitioners who, for ease of use and availability, prefer to work with commercial extenders.
authors: Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Cordoba, Cordoba, Spain.
authors: E
authors: E
authors: Jiménez
authors: C C
authors: CC
authors: Pérez-Marín
authors: G
authors: G
authors: Vizuete
authors: Y
authors: Y
authors: Millán
authors: E I
authors: EI
authors: Agüera
mesh_terms: D000818:Animals; D002415:Cats / Q000502:physiology*; D002417:Cattle; D002462:Cell Membrane / Q000502:physiology; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004530:Egg Yolk; D004822:Epididymis / Q000166:cytology*; D006736:Horses; D007552:Isotonic Solutions; D008297:Male; D055101:Semen Analysis / Q000662:veterinary; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary; D013076:Sperm Count / Q000662:veterinary; D013081:Sperm Motility; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology* / Q000648:ultrastructure; D014325:Tromethamine
chemical_list: D003451:Cryoprotective Agents; D007552:Isotonic Solutions; C503302:triladyl; D014325:Tromethamine