title: Cytokinins can act as suppressors of nitric oxide in Arabidopsis.
abstract: Maintaining nitric oxide (NO) homeostasis is essential for normal plant physiological processes. However, very little is known about the mechanisms of NO modulation in plants. Here, we report a unique mechanism for the catabolism of NO based on the reaction with the plant hormone cytokinin. We screened for NO-insensitive mutants in Arabidopsis and isolated two allelic lines, cnu1-1 and 1-2 (continuous NO-unstressed 1), that were identified as the previously reported altered meristem program 1 (amp1) and as having elevated levels of cytokinins. A double mutant of cnu1-2 and nitric oxide overexpression 1 (nox1) reduced the severity of the phenotypes ascribed to excess NO levels as did treating the nox1 line with trans-zeatin, the predominant form of cytokinin in Arabidopsis. We further showed that peroxinitrite, an active NO derivative, can react with zeatin in vitro, which together with the results in vivo suggests that cytokinins suppress the action of NO most likely through direct interaction between them, leading to the reduction of endogenous NO levels. These results provide insights into NO signaling and regulation of its bioactivity in plants.
authors: College of Life Sciences and Department of Chemistry,  Capital Normal University, Beijing 100048,  China.
authors: Wei-Zhong
authors: WZ
authors: Liu
authors: Dong-Dong
authors: DD
authors: Kong
authors: Xue-Xin
authors: XX
authors: Gu
authors: Hong-Bo
authors: HB
authors: Gao
authors: Jin-Zheng
authors: JZ
authors: Wang
authors: Min
authors: M
authors: Xia
authors: Qian
authors: Q
authors: Gao
authors: Li-Li
authors: LL
authors: Tian
authors: Zhang-Hong
authors: ZH
authors: Xu
authors: Fang
authors: F
authors: Bao
authors: Yong
authors: Y
authors: Hu
authors: Neng-Sheng
authors: NS
authors: Ye
authors: Zhen-Ming
authors: ZM
authors: Pei
authors: Yi-Kun
authors: YK
authors: He
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002268:Carboxypeptidases / Q000235:genetics / Q000378:metabolism; D003583:Cytokinins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D035264:Flowers / Q000254:growth & development / Q000378:metabolism; D017343:Genes, Plant; D009154:Mutation; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D030421:Peroxynitrous Acid / Q000378:metabolism; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D015026:Zeatin / Q000378:metabolism / Q000494:pharmacology
chemical_list: D029681:Arabidopsis Proteins; D003583:Cytokinins; D020030:Nitric Oxide Donors; D012333:RNA, Messenger; D018749:RNA, Plant; D030421:Peroxynitrous Acid; D009599:Nitroprusside; D009569:Nitric Oxide; D015026:Zeatin; D002268:Carboxypeptidases; C506681:AMP1 protein, Arabidopsis