title: In vitro pathogenicity assay for anti-desmoglein autoantibodies in pemphigus.
abstract: Patients with pemphigus have circulating anti-desmoglein (Dsg) 1 and/or Dsg3 autoantibodies that induce blister formation on the skin and mucous membrane. We describe here two assays that measure the pathogenic strength of autoantibodies in blister formation: an in vitro dissociation assay using primary human epidermal keratinocytes to assess pathogenicity of anti-Dsg3 autoantibodies, and an alternative method whereby anti-Dsg3 and Dsg1 autoantibodies are injected into organ-cultured human skin specimen.
authors: Department of Dermatology, Toho University, School of Medicine, Tokyo, Japan. k-ishii@med.teikyo-u.ac.jp
authors: Ken
authors: K
authors: Ishii
authors: Masayuki
authors: M
authors: Amagai
mesh_terms: D001323:Autoantibodies / Q000276:immunology*; D001768:Blister / Q000150:complications / Q000276:immunology / Q000473:pathology*; D002478:Cells, Cultured; D051183:Desmoglein 1 / Q000276:immunology*; D051184:Desmoglein 3 / Q000276:immunology*; D003896:Desmosomes / Q000276:immunology / Q000473:pathology; D006801:Humans; D015603:Keratinocytes / Q000276:immunology / Q000473:pathology; D009924:Organ Culture Techniques / Q000379:methods; D010392:Pemphigus / Q000150:complications / Q000276:immunology / Q000473:pathology*; D012867:Skin / Q000276:immunology / Q000473:pathology*
chemical_list: D001323:Autoantibodies; D051183:Desmoglein 1; D051184:Desmoglein 3