title: Construction of homologous and heterologous synthetic sucrose utilizing modules and their application for carotenoid production in recombinant Escherichia coli.
abstract: Sucrose is one of the most promising carbon sources for industrial fermentation. We expressed synthetic modules expressing genes of the PEP-PTS and non-PTS pathways in Escherichia coli K12 for comparison. We selected PEP-PTS pathway genes of Lactobacillus plantarum and Staphylococcus xylosus and non-PTS pathway genes of sucrose-utilizing (Scr(+)) E. coli EC3132. Switchable Scr(+) modules expressing E. coli EC3132 non-PTS genes conferred better sucrose-utilizing ability on Scr(-)E. coli K12 than E. coli EC3132. Scr(+) modules expressing S. xylosus PEP-PTS genes conferred a sucrose-utilizing ability on E. coli K12. Among L. plantarum PEP-PTS genes, SacA(LP) and SacK(LP) were functional in E. coli K12. CscA(EC)-CscB(EC)-CscK(EC) (non-PEP-PTS module) or ScrA(SX)-SacA(LP)-SacK(LP) (PEP-PTS module) was introduced to a diapolycopene-producing E. coli strain. In both Scr(+)E. coli K12, the sucrose-utilizing ability of the modules was not affected by diapolycopene formation, indicating that the modular Scr(+) systems could be employed for developing sustainable bioprocesses using sucrose.
authors: Department of Molecular Science and Technology, Ajou University, Woncheon-dong, Yeongtong-gu, Suwon 443-749, Republic of Korea.
authors: Jong Rae
authors: JR
authors: Kim
authors: Se Hyeuk
authors: SH
authors: Kim
authors: Sang Yup
authors: SY
authors: Lee
authors: Pyung Cheon
authors: PC
authors: Lee
mesh_terms: D002338:Carotenoids / Q000096:biosynthesis*; D049109:Cell Proliferation; D048168:Escherichia coli K12 / Q000235:genetics / Q000378:metabolism*; D053263:Gene Regulatory Networks; D048191:Lactiplantibacillus plantarum / Q000201:enzymology / Q000235:genetics; D010728:Phosphoenolpyruvate / Q000378:metabolism*; D010770:Phosphotransferases / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013210:Staphylococcus / Q000201:enzymology / Q000235:genetics; D013395:Sucrose / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D002338:Carotenoids; D013395:Sucrose; D010728:Phosphoenolpyruvate; D010770:Phosphotransferases