title: Isolation and characterization of  25 microsatellite DNA loci for  Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species.
abstract: The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated alleles ranged from 2 to 10, the observed heterozygosity ranged from 0.182 to 0.897, and the expected heterozygosity ranged from 0.260 to 0.854. Eleven loci showed significant deviation from Hardy-Weinberg equilibrium. Eleven loci were cross-amplified successfully in 5 Anopheles species. These microsatellite loci will be useful in studies investigating population structure and evolutionary genetics in A. albitarsis sensu lato and other A. albitarsis complex species.
authors: Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiva, Instituto Nacional de Pesquisa da Amazônia, Manaus, AM, Brasil.
authors: G M
authors: GM
authors: Guimarães-Marques
authors: J S
authors: JS
authors: Batista
authors: H M
authors: HM
authors: Guimarães
authors: M V
authors: MV
authors: Naice-Daou
authors: M P
authors: MP
authors: Lima
authors: K M
authors: KM
authors: Formiga
authors: J M M
authors: JM
authors: Santos
authors: C A P
authors: CA
authors: Lima
authors: M S
authors: MS
authors: Rafael
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics*; D017344:Genes, Insect; D056426:Genetic Loci; D005838:Genotype; D007303:Insect Vectors / Q000235:genetics*; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity