title: Thy-1 knockdown retards wound repair in mouse skin.
abstract: BACKGROUND
  Thy-1 (CD90) is a glycophosphatidyl-inositol (GPI) linked, cell surface glycoprotein located in non-caveolar lipid raft microdomains.
  
  
  OBJECTIVE
  The biological functions of Thy-1 in many tissues are well known, however, its role in skin wound healing remains unclear.
  
  
  RESULTS
  In fibroblasts cells, Thy-1 affects cell migration into the wound, cell proliferation and the cytoskeleton structure. Additionally, Thy-1 is mainly expressed in the wound dermis. Here, we compared the in vivo aspects of the repair process with and without Thy-1 siRNA treatment. Temporally blocking Thy-1 in skin wound regions worsens the quality of healing and retards the rate of wound healing. Specifically, the level of TGF-β1 at the wound continuously increased.
  
  
  CONCLUSIONS
  These data suggest that blocking Thy-1 at wound areas using siRNA reduces repair and affects the re-epithelialization and over-expression of TGF-β1 of the wound during the skin healing process.
authors: Division in Anatomy and Developmental Biology, Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 Project, Oral Science Research Center, College of Dentistry, Yonsei Center of Biotechnology, Yonsei University, Seoul, Republic of Korea.
authors: Min-Jung
authors: MJ
authors: Lee
authors: Jeong-Oh
authors: JO
authors: Shin
authors: Han-Sung
authors: HS
authors: Jung
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D008297:Male; D021962:Membrane Microdomains / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR; D018345:Mice, Knockout; D041681:NIH 3T3 Cells; D034741:RNA, Small Interfering / Q000235:genetics; D012867:Skin / Q000293:injuries*; D012879:Skin Physiological Phenomena; D018800:Thy-1 Antigens / Q000235:genetics* / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology*
chemical_list: D034741:RNA, Small Interfering; D018800:Thy-1 Antigens; D053773:Transforming Growth Factor beta1