title: Netrin-1 pretreatment protects rat kidney against ischemia/reperfusion injury via suppression of oxidative stress and neuropeptide Y expression.
abstract: Netrin-1 has been found to protect kidneys from ischemia/reperfusion injury. In this study, we aimed to address whether the protective effects were mediated through suppression of oxidative stress and neuropeptide Y. Compared to sham-operated animals, animals after ischemia/reperfusion showed marked kidney damage and significantly increased levels of serum creatinine, blood urea nitrogen, malondialdehyde, and neuropeptide Y. Renal myeloperoxidase activity was elevated in animals with ischemia/reperfusion relative to sham-operated animals, whereas renal superoxide dismutase activity was reduced. Netrin-1 pretreatment attenuated ischemia/reperfusion-induced functional and pathological changes in the kidney. Moreover, the ischemia/reperfusion-induced changes in the oxidative stress biomarkers and neuropeptide Y were significantly counteracted by prior administration of netrin-1. Taken together, our data showed that netrin-1 pretreatment prevented renal ischemia/reperfusion injury, at least partially through reduction of oxidative stress and neuropeptide Y expression.
authors: Department of Nephrology, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People's Republic of China.
authors: Ling
authors: L
authors: Liu
authors: Shuqin
authors: S
authors: Xie
authors: Xiaohui
authors: X
authors: Liao
authors: Ling
authors: L
authors: Zhang
authors: Ling
authors: L
authors: Zhong
mesh_terms: D000818:Animals; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D007677:Kidney Function Tests; D008297:Male; D009414:Nerve Growth Factors / Q000494:pharmacology / Q000627:therapeutic use*; D000075388:Netrin-1; D009478:Neuropeptide Y / Q000097:blood / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood / Q000188:drug therapy* / Q000473:pathology* / Q000503:physiopathology; D025521:Tumor Suppressor Proteins / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: C000617351:NTN1 protein, human; D009414:Nerve Growth Factors; D009478:Neuropeptide Y; C000617354:Ntn1 protein, rat; D020011:Protective Agents; D025521:Tumor Suppressor Proteins; D000075388:Netrin-1; D003404:Creatinine