title: Correlation between auto-antibodies to survivin and MUC1 variable number tandem repeats in colorectal cancer.
abstract: OBJECTIVE
  The aim of this study was to investigate the frequency and correlation between auto-antibodies to survivin and MUC1 variable number tandem repeats (VNTR) in colorectal cancer (CRC), which can provide valuable information for the design of immunotherapeutic vaccines for this disease.
  
  
  METHODS
  Enzyme-linked immunosorbent assays (ELISA) were used to examine the level of auto-antibodies against survivin and MUC1 VNTR in the serum of 135 CRC patients and 95 healthy volunteers.
  
  
  RESULTS
  Using mean absorbance+2 standard deviations (SD) of the healthy samples as a cut-off value, the positive rates of survivin and MUC1 VNTR auto- antibodies in CRC were 31.1% and 18.5%, respectively. Altogether, the survivin and MUC1 VNTR positive samples accounted for 36.3% of the CRC patients, and 7.4% were positive for both.
  
  
  CONCLUSIONS
  A significant positive correlation was found between levels of specific antibodies against survivin and MUC1 VNTR in the serum of CRC patients (r=0.3652, P<0.0001), suggesting that vaccines against both targets would elicit immune responses more effectively.
authors: National Engineering Laboratoty of AIDS Vaccine, College of Life Science, Jilin University, Changchun, China.
authors: Yu-Qian
authors: YQ
authors: Wang
authors: Hai-Hong
authors: HH
authors: Zhang
authors: Chen-Lu
authors: CL
authors: Liu
authors: Qiu
authors: Q
authors: Xia
authors: Hui
authors: H
authors: Wu
authors: Xiang-Hui
authors: XH
authors: Yu
authors: Wei
authors: W
authors: Kong
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000097:blood*; D015153:Blotting, Western; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D018598:Minisatellite Repeats / Q000276:immunology*; D018396:Mucin-1; D011994:Recombinant Proteins / Q000276:immunology; D000077022:Survivin
chemical_list: D001323:Autoantibodies; C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; C060192:MUC1 protein, human; D018396:Mucin-1; D011994:Recombinant Proteins; D000077022:Survivin