title: Structural insights into protein-only RNase P complexed with tRNA.
abstract: RNase P is the essential activity removing 5'-leader sequences from transfer RNA precursors. RNase P was always associated with ribonucleoprotein complexes before the discovery of protein-only RNase P enzymes called PRORPs (PROteinaceous RNase P) in eukaryotes. Here we provide biophysical and functional data to understand the mode of action of PRORP enzymes. Activity assays and footprinting experiments show that the anticodon domain of transfer RNA is dispensable, whereas individual residues in D and TψC loops are essential for PRORP function. PRORP proteins are characterized in solution and a molecular envelope is derived from small-angle X-ray scattering. Conserved residues are shown to be involved in the binding of one zinc atom to PRORP. These results facilitate the elaboration of a model of the PRORP/transfer RNA interaction. The comparison with the ribonucleoprotein RNase P/transfer RNA complex suggests that transfer RNA recognition by PRORP proteins is similar to that by ribonucleoprotein RNase P.
authors: Institut de Biologie Moléculaire des Plantes du CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg, France.
authors: Anthony
authors: A
authors: Gobert
authors: Franziska
authors: F
authors: Pinker
authors: Olivier
authors: O
authors: Fuchsbauer
authors: Bernard
authors: B
authors: Gutmann
authors: René
authors: R
authors: Boutin
authors: Pierre
authors: P
authors: Roblin
authors: Claude
authors: C
authors: Sauter
authors: Philippe
authors: P
authors: Giegé
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000378:metabolism*; D008954:Models, Biological; D008958:Models, Molecular; D011485:Protein Binding; D012313:RNA / Q000737:chemistry; D012322:RNA Precursors / Q000737:chemistry; D000077278:RNA, Mitochondrial; D018749:RNA, Plant / Q000737:chemistry / Q000378:metabolism; D012343:RNA, Transfer / Q000737:chemistry* / Q000378:metabolism; D011995:Recombination, Genetic / Q000235:genetics; D038621:Regulatory Sequences, Ribonucleic Acid; D043262:Ribonuclease P / Q000737:chemistry* / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000737:chemistry / Q000378:metabolism; D053838:Scattering, Small Angle; D012996:Solutions; D013054:Spectrophotometry, Atomic; D014961:X-Ray Diffraction; D015032:Zinc / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D012322:RNA Precursors; D000077278:RNA, Mitochondrial; D018749:RNA, Plant; D038621:Regulatory Sequences, Ribonucleic Acid; D012261:Ribonucleoproteins; D012996:Solutions; D012313:RNA; D012343:RNA, Transfer; C581753:PRORP1 protein, Arabidopsis; C581754:PRORP2 protein, Arabidopsis; D043262:Ribonuclease P; D015032:Zinc