title: Versatile biofunctionalization of polypeptide-based thermosensitive hydrogels via click chemistry.
abstract: In this study, we report thermosensitive hydrogels based on poly(ethylene glycol)-block-poly(γ-propargyl-l-glutamate) (PEG-PPLG). (13)C NMR spectra, DLS, and circular dichroism spectra were employed to study the mechanism of the sol-gel phase transition. Mouse fibroblast L929 cells were encapsulated and cultured within the hydrogel matrices, and the encapsulated cells were shown to be highly viable in the gel matrices, suggesting that the hydrogels have excellent cytocompatibilities. The mass loss of the hydrogels in vitro was accelerated by the presence of proteinase K compared to the control group. In vivo biocompatibility studies revealed that the in situ formed gels in the subcutaneous layer last for ∼21 days, and H&E staining study suggested acceptable biocompatibility of our materials in vivo. The presence of alkynyl side groups in the PEG-PPLG copolymers allowed convenient further functionalization with azide-modified bioactive molecules, such as biotin and galactose. The biofunctionalized PEG-polypeptide block copolymers showed sol-gel phase transitions similar to the parent copolymers. Interestingly, the incorporation of galactose groups into the hydrogels was found to improve cell adhesion, likely due to the adsorption of fibronectin (FN) in cell-extracellular matrix (ECM). Because bioactive materials have shown unique advantages in biomedical applications, especially tissue engineering and regenerative medicine applications, we believe our novel functionalizable thermosensitive hydrogels have potential to serve as a versatile platform for the development of new biofunctional materials, for example, bioadhesive and bioresponsive hydrogels.
authors: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, P. R. China.
authors: Yilong
authors: Y
authors: Cheng
authors: Chaoliang
authors: C
authors: He
authors: Chunsheng
authors: C
authors: Xiao
authors: Jianxun
authors: J
authors: Ding
authors: Haitao
authors: H
authors: Cui
authors: Xiuli
authors: X
authors: Zhuang
authors: Xuesi
authors: X
authors: Chen
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002460:Cell Line; D057930:Click Chemistry; D005109:Extracellular Matrix / Q000378:metabolism; D005353:Fibronectins / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D010455:Peptides; D044367:Phase Transition; D011092:Polyethylene Glycols / Q000737:chemistry*; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D005353:Fibronectins; D020100:Hydrogels; D010455:Peptides; D011108:Polymers; C582614:poly(ethylene glycol)-block-poly(gamma-propargylglutamate); D011099:Polyglutamic Acid; D011092:Polyethylene Glycols