title: Achyranthes bidentata polysaccharides induce chondrocyte proliferation             via the promotion of the G1/S cell cycle transition.
abstract: Achyranthes bidentata polysaccharides (ABPS) are the major bioactive constituents             of Radix Achyranthes bidentata (AB), which has been widely used in traditional             Chinese medicine for the treatment of osteoarthritis. However, the molecular mechanisms             behind the therapeutic effect of ABPS remain unclear. In the present study, chondrocytes             were isolated from Sprague-Dawley rats. The effects of ABPS on the G1/S cell cycle             transition in primary chondrocytes were investigated. The chondrocytes treated             with and without ABPS were analyzed and it was observed that ABPS treatment was             able to enhance chondrocyte proliferation in a dose- and time-dependent manner             and promote the progression of chondrocyte cell cycle proliferation via the promotion             of the G1 to S phase transition. Furthermore, using RT-PCR and western blot analysis,             ABPS were observed as significantly upregulating the expression of cyclin D1 and             the cyclin-dependent kinases (CDKs) CDK4 and CDK6. These results suggest that             ABPS are able to promote chondrocyte proliferation via the promotion of the G1/S             cell cycle transition.
authors: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, PR China.
authors: Fangrong
authors: F
authors: Yu
authors: Xihai
authors: X
authors: Li
authors: Liangliang
authors: L
authors: Cai
authors: Huiting
authors: H
authors: Li
authors: Jiashou
authors: J
authors: Chen
authors: Xiaping
authors: X
authors: Wong
authors: Huifeng
authors: H
authors: Xu
authors: Chunsong
authors: C
authors: Zheng
authors: Xianxiang
authors: X
authors: Liu
authors: Hongzhi
authors: H
authors: Ye
mesh_terms: D029044:Achyranthes / Q000737:chemistry; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D051358:Cyclin-Dependent Kinase 4 / Q000235:genetics / Q000378:metabolism; D051361:Cyclin-Dependent Kinase 6 / Q000235:genetics / Q000378:metabolism; D016193:G1 Phase / Q000187:drug effects; D008297:Male; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D016196:S Phase / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D011134:Polysaccharides; D012333:RNA, Messenger; D019938:Cyclin D1; D051358:Cyclin-Dependent Kinase 4; D051361:Cyclin-Dependent Kinase 6