title: [The relationship between C20orf54 gene rs3746804 position single nucleotide polymorphism and susceptibility to esophageal squamous cell carcinoma].
abstract: OBJECTIVE
  To explore the association of C20orf54 gene rs3746804 position single nucleotide polymorphism and susceptibility to esophageal squamous cell carcinoma (ESCC).
  
  
  METHODS
  Purification of genomic DNA from whole blood was used the Maxwell(16) System. rs3746804 in C20orf54 was detected by direct sequencing in 434 ESCC patients from Changzhi (Shanxi province) and Linzhou (Henan province) and 554 healthy controls from Changzhi, Linzhou and including immigrators from Linzhou to Changzhi.
  
  
  RESULTS
  For rs3746804, the genotypic frequencies of CT (37.5% vs 51.0%, 37.5% vs 52.0%), CC (44.2% vs 34.8%, 44.2% vs 33.0%) in Changzhi ESCC patients showed significant differences with healthy Changzhi controls and the healthy immigrator controls (all P < 0.05), and the frequencies of TT (18.3% vs 4.1%) and CC (44.2% vs 54.6%) in Changzhi ESCC patients showed significant differences with Linzhou ESCC patients (all P < 0.05). The genotypic frequencies of TT (4.1% vs 15.0%), CT (41.2% vs 52.0%) and CC(54.6% vs 33.0%) showed significant differences between Linzhou ESCC patients and the healthy immigrator controls (all P < 0.05), and the frequencies of TT (4.1% vs 14.1%) and CC(54.6% vs 34.8%) showed significant differences between Linzhou ESCC patients and Changzhi healthy controls (all P < 0.01). Meanwhile, there were significant differences between ESCC patients (including Changzhi and Linzhou ESCC patients) and healthy controls (including the healthy Changzhi, Linzhou and immigrator controls) in genotypic frequencies of CT (39.2% vs 48.7%) and CC (48.8% vs 38.2%) (all P < 0.01). CT and CT + TT genotype could decrease the risk of ESCC compared with the CC genotype (OR = 0.630, 95%CI 0.481 - 0.826; OR = 0.654, 95%CI 0.507 - 0.844).
  
  
  CONCLUSIONS
  There is a closed relationship between SNP rs3746804 in C20orf54 and susceptibility to ESCC.
authors: Zhengzhou University, Zhengzhou, China.
authors: Ai-fang
authors: AF
authors: Ji
authors: Wu
authors: W
authors: Wei
authors: Jian-zhou
authors: JZ
authors: Yang
authors: Jin-sheng
authors: JS
authors: Wang
authors: Li
authors: L
authors: Zhao
authors: Zi-bai
authors: ZB
authors: Wei
authors: Chang-hong
authors: CH
authors: Lian
authors: Liang
authors: L
authors: Ma
authors: Li
authors: L
authors: Ma
authors: Hai-li
authors: HL
authors: Wang
authors: Xiao-qi
authors: XQ
authors: Qin
authors: Li-dong
authors: LD
authors: Wang
mesh_terms: D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D016022:Case-Control Studies; D004938:Esophageal Neoplasms / Q000235:genetics*; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012256:Riboflavin / Q000378:metabolism
chemical_list: D026901:Membrane Transport Proteins; C548553:SLC52A3 protein, human; D012256:Riboflavin