title: Localization of Rab proteins to peroxisomes: a proteomics and immunofluorescence study.
abstract: A proteomics screen was initiated to identify Rab proteins regulating transport to and away from peroxisomes. Mass spectrometry-based protein correlation profiling of rat liver organelles and immunofluorescence analysis of the peroxisome candidate Rab proteins revealed Rab6, Rab10, Rab14 and Rab18 to associate with the peroxisomal membrane. While Rab14 localized to peroxisomes predominantly in its dominant-active form, other Rab proteins associated with peroxisomes in both their GTP- and GDP-bound state. In summary, our data suggest that Rab6, Rab10, Rab14 and Rab18 associate with the peroxisomal compartment and similar as previously shown for Rab8, Rab18 in its GDP-bound state favors peroxisome proliferation.
authors: Department of Molecular Genetics and Cell Biology, Ulm University, Germany.
authors: Thomas
authors: T
authors: Gronemeyer
authors: Sebastian
authors: S
authors: Wiese
authors: Sören
authors: S
authors: Grinhagens
authors: Lukas
authors: L
authors: Schollenberger
authors: Ari
authors: A
authors: Satyagraha
authors: Lukas A
authors: LA
authors: Huber
authors: Helmut E
authors: HE
authors: Meyer
authors: Bettina
authors: B
authors: Warscheid
authors: Wilhelm W
authors: WW
authors: Just
mesh_terms: D000818:Animals; D002460:Cell Line; D006153:Guanosine Diphosphate / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D020675:Peroxisomes / Q000201:enzymology* / Q000378:metabolism; D021381:Protein Transport; D040901:Proteomics / Q000379:methods; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015854:Up-Regulation; D020691:rab GTP-Binding Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*
chemical_list: D008164:Luminescent Proteins; D050505:Mutant Proteins; C083879:Rab18 protein, mouse; C094135:Rab6 protein; D011993:Recombinant Fusion Proteins; D006153:Guanosine Diphosphate; C538901:Rab10 protein, human; C553411:Rab14 protein, human; C522121:Rab18 protein, rat; D020691:rab GTP-Binding Proteins