title: Role of a repeated hexapeptide motif GIHFAP near C-terminus in assembly, stability, and activity of "HCH dehydrochlorinase LinA".
abstract: Enzyme "hexachlorocyclohexane (HCH) dehydrochlorinase LinA" mediates first step of aerobic microbial degradation of a chlorinated insecticide γ-HCH. The archetypal LinA-type1 consists of 156 amino acids that include a directly repeated hexapeptide motif GIHFAP at positions 141-146 and 148-153. Analysis of a series of LinA mutants, containing none, one, two, or three units of this repeated motif revealed that two units, as present in wild-type LinA, are required for its optimal activity and stability. Moreover, the presence of a bend in its secondary structure due to a proline residue that precedes the distal repeated unit contributes to enhanced LinA activity.
authors: Environmental Biotechnology Section, Indian Institute of Toxicology Research (Council of Scientific and Industrial Research), Mahatma Gandhi Marg, Lucknow, 226001, India.
authors: Ankit S
authors: AS
authors: Macwan
authors: Nidhi
authors: N
authors: Srivastava
authors: Saleem
authors: S
authors: Javed
authors: Ashwani
authors: A
authors: Kumar
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D002850:Chromatography, Gel; D004795:Enzyme Stability; D001556:Hexachlorocyclohexane / Q000378:metabolism*; D008190:Lyases / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data
chemical_list: D001426:Bacterial Proteins; D001556:Hexachlorocyclohexane; D008190:Lyases