title: [The comparison of platelet-rich fibrin and platelet-rich plasma in releasing of growth factors and their effects on the proliferation and differentiation of adipose tissue-derived stem cells in vitro].
abstract: OBJECTIVE
  To compare the releasing of growth factors between platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) as well as their effects on the proliferation and differentiation of adipose tissue-derived stem cells (ADSCs) in vitro.
  
  
  METHODS
  Blood was taken from central artery of rabbits, acquiring PRF was acquired through one time centrifuge and PRP through two times centrifuge. Five milliliters of fresh alpha-MEM was added to PRF and PRP and incubated at 37 degrees C. The time points to collect exudates was in day 1, 7, 14, 21, 28 and the mass concentrations of transforming growth factor-beta1 (TGF-beta1) and platelet derived growth factor-AB(PDGF-AB) were quantified in PRF and PRP. Then the exudates of PRF and PRP were used to culture ADSCs and evaluate the effects of PRF and PRP on proliferation and differentiation of ADSCs.
  
  
  RESULTS
  1) Growth factor release: In the PRF exudates at different time points, the mass concentration of TGF-beta1 was the highest at day 14 and the highest mass concentration of PDGF-AB at day 7, the mass concentration of both TGF-beta1 and PDGF-AB was the highest at the first day and then gradually declined. 2)The effect on proliferation and differentiation: PRF exudates of day 14 expressed the maximum proliferation and alkaline phosphatase (ALP) activity. PRF exudates of day 1 demonstrat the maximum proliferation and ALP activity.
  
  
  CONCLUSIONS
  Comparing to PRP, PRF releases growth factors gradually and expressed stronger and more durable effect on proliferation and differentiation of ADSCs in vitro.
authors: Dept. of Oral and Maxillofacial Surgery, School of Stomatology, Shandong University, Jinan 250012, China.
authors: Mingguo
authors: M
authors: Wang
authors: Jing
authors: J
authors: Li
authors: Jinpan
authors: J
authors: Liu
authors: Xialian
authors: X
authors: Lin
authors: Wei
authors: W
authors: Xu
mesh_terms: D000273:Adipose Tissue; D001792:Blood Platelets; D002454:Cell Differentiation; D005337:Fibrin; D066298:In Vitro Techniques; D036341:Intercellular Signaling Peptides and Proteins; D009930:Organic Chemicals; D010982:Platelet-Derived Growth Factor; D053657:Platelet-Rich Plasma; D013234:Stem Cells; D053773:Transforming Growth Factor beta1
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D009930:Organic Chemicals; D010982:Platelet-Derived Growth Factor; D053773:Transforming Growth Factor beta1; C420642:alpha minimal essential medium; C095477:platelet-derived growth factor AB; D005337:Fibrin