title: Soluble CD146 in cerebrospinal fluid of active multiple sclerosis.
abstract: The soluble form of CD146 has been reported to be present in various inflammatory diseases and displays pro-inflammatory properties. However, little is known about sCD146 in multiple sclerosis (MS). Here we show that sCD146 is significantly elevated in the cerebrospinal fluid of patients with active MS compared with that of inactive MS or patients with non-demyelinating diseases. Moreover, abnormally increased sCD146 in the CSF of active MS patients correlated with albumin quotient, MBP antibody and MOG antibody from both CSF and sera. Importantly, the level of CSF sCD146 is correlated with levels of inflammatory factors, such as TNFα, IFNγ, IL-2, and IL-17A in the CSF. We also found that CSF sCD146 might originate from membrane-bound CD146 on inflamed blood-brain barrier (BBB) endothelial cells. In addition, sCD146 promotes leukocyte transmigration in vitro, at least in part by stimulating the expression of ICAM-1 and VCAM-1 on endothelial cells. Our findings suggest that CSF levels of sCD146 may provide a potential marker for monitoring disease activity in MS patients.
authors: Key Laboratory of Protein and Peptide Pharmaceutical, CAS-University of Tokyo Joint Laboratory of Structural Virology and Immunology, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China.
authors: H
authors: H
authors: Duan
authors: Y
authors: Y
authors: Luo
authors: H
authors: H
authors: Hao
authors: L
authors: L
authors: Feng
authors: Y
authors: Y
authors: Zhang
authors: D
authors: D
authors: Lu
authors: S
authors: S
authors: Xing
authors: J
authors: J
authors: Feng
authors: D
authors: D
authors: Yang
authors: L
authors: L
authors: Song
authors: X
authors: X
authors: Yan
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000134:cerebrospinal fluid; D001812:Blood-Brain Barrier / Q000502:physiology; D015153:Blotting, Western; D051929:CD146 Antigen / Q000134:cerebrospinal fluid*; D054441:Cell Migration Assays, Leukocyte; D016207:Cytokines / Q000134:cerebrospinal fluid; D042783:Endothelial Cells / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007202:Indicators and Reagents; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007525:Isoelectric Focusing; D008297:Male; D008566:Membranes / Q000378:metabolism; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid*; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
chemical_list: D015415:Biomarkers; D051929:CD146 Antigen; D016207:Cytokines; D007202:Indicators and Reagents; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1