title: Intestinal proteome changes during infant necrotizing enterocolitis.
abstract: BACKGROUND
  Changes in the intestinal and colonic proteome in patients with necrotizing enterocolitis (NEC) may help to characterize the disease pathology and identify new biomarkers and treatment targets for NEC.
  
  
  METHODS
  Using gel-based proteomics, proteins in NEC-affected intestinal and colonic sections were compared with those in adjacent, near-normal tissue sections within the same patients. Western blot and immunohistochemistry were applied to crossvalidate proteomic data and histological location of some selected proteins.
  
  
  RESULTS
  Thirty proteins were identified with differential expression between necrotic and vital small-intestine sections and 23 proteins were identified for colon sections. Five proteins were similarly affected in the small intestine and colon: histamine receptors (HRs), actins, globins, immunoglobulin, and antitrypsin. Two heat shock proteins (HSPs) were affected in the small intestine. Furthermore, proteins involved in antioxidation, angiogenesis, cytoskeleton formation, and metabolism were affected. Finally, secretory proteins such as antitrypsin, fatty-acid binding protein 5, and haptoglobin differed between NEC-affected and vital tissues.
  
  
  CONCLUSIONS
  NEC progression affects different pathways in the small intestine and colon. HSPs may play an important role, especially in the small intestine. The identified secretory proteins should be investigated as possible circulating markers of NEC progression in different gut regions.
authors: School of Biological Sciences, University of Hong Kong, Hong Kong, China.
authors: Pingping
authors: P
authors: Jiang
authors: Birgitte
authors: B
authors: Smith
authors: Niels
authors: N
authors: Qvist
authors: Christian
authors: C
authors: Nielsen
authors: Jennifer Man-Fan
authors: JM
authors: Wan
authors: Wai-Hung
authors: WH
authors: Sit
authors: Tim Kåre
authors: TK
authors: Jensen
authors: Hualin
authors: H
authors: Wang
authors: Per Torp
authors: PT
authors: Sangild
mesh_terms: D000199:Actins / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism*; D015153:Blotting, Western; D003718:Denmark; D020345:Enterocolitis, Necrotizing / Q000175:diagnosis / Q000378:metabolism*; D005914:Globins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D007150:Immunohistochemistry; D007231:Infant, Newborn; D007413:Intestinal Mucosa / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D011968:Receptors, Histamine / Q000378:metabolism
chemical_list: D000199:Actins; D015415:Biomarkers; D006360:Heat-Shock Proteins; D007136:Immunoglobulins; D020543:Proteome; D011968:Receptors, Histamine; D005914:Globins