title: Association analyses of osteoprotegerin gene polymorphisms with bone mineral density in Chinese postmenopausal women.
abstract: Osteoprotegerin gene (OPG) is an important candidate gene of osteoporosis. The objective of this study was to evaluate the association between OPG gene polymorphisms and bone mineral density (BMD). A total of 336 Chinese postmenopausal women were included in this study. OPG gene polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), created restriction site-PCR (CRS-PCR) and DNA sequencing methods. BMD was evaluated at the lumbar spine (L(2-4)), total hip and femoral neck. Two single-nucleotide polymorphisms (SNPs) (g.21775C>T and g.23367T>C) were identified and the association analysis showed significant difference of spine BMD among different g.23367T>C genotype, subjects with the genotype TT was significantly higher than those of genotype TC and CC. Such a significant difference was not observed at the neck hip BMD and total hip BMD. The g.21775C>T polymorphism was not significantly associated with spine BMD, total hip BMD and neck hip BMD in the studied subjects. These findings suggested that OPG gene polymorphisms were associated with BMD in Chinese postmenopausal women. Results from this study will be helpful in further studies to determine the role of OPG gene in osteoporosis.
authors: Orthopedics Department, The Second Affiliated Hospital Zhejiang University College of Medicine, No. 88 Jiefang Road, Hangzhou 310009, People's Republic of China.
authors: Feng
authors: F
authors: Zhang
authors: Chunlei
authors: C
authors: He
authors: Gang
authors: G
authors: Chen
authors: Fangcai
authors: F
authors: Li
authors: Hui
authors: H
authors: Gao
mesh_terms: D015502:Absorptiometry, Photon; D000368:Aged; D044466:Asian People / Q000235:genetics; D015519:Bone Density / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D015663:Osteoporosis, Postmenopausal / Q000235:genetics*; D053244:Osteoprotegerin / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D017698:Postmenopause / Q000235:genetics*
chemical_list: D053244:Osteoprotegerin; C506030:TNFRSF11B protein, human