title: HSPRO acts via SnRK1-mediated signaling in the regulation of Nicotiana attenuata seedling growth promoted by Piriformospora indica.
abstract: Nicotiana attenuata HSPRO (NaHSPRO) is a negative regulator of seedling growth promoted by the fungus Piriformospora indica. Homologs of NaHSPRO in Arabidopsis thaliana (i.e., AtHSPRO1 and AtHSPRO2) are known to physically interact with the AKINβγ subunit of the SnRK1 complex. To investigate whether NaHSPRO is associated with SnRK1 function during the stimulation of seedling growth by P. indica, we studied N. attenuata plants silenced in the expression of NaGAL83 (as-gal83 plants)--a gene that encodes for the regulatory β-subunit of SnRK1--and plants silenced in the expression of both NaHSPRO and NaGAL83 (ir-hspro/as-gal83 plants). The results showed that P. indica differentially stimulated the growth of both as-gal83 and ir-hspro/as-gal83 seedlings compared with control seedlings, with a magnitude similar to that observed in ir-hspro seedlings. Thus, we showed that, similar to NaHSPRO, NaGAL83 is a negative regulator of seedling growth stimulated by P. indica. We propose that the effect of NaHSPRO on seedling growth is associated with SnRK1 signaling.
authors: Department of Molecular Ecology; Max Planck Institute for Chemical Ecology; Jena, Germany.
authors: Stefan
authors: S
authors: Schuck
authors: Ian T
authors: IT
authors: Baldwin
authors: Gustavo
authors: G
authors: Bonaventure
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001487:Basidiomycota; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D017343:Genes, Plant; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000254:growth & development*; D015398:Signal Transduction; D013559:Symbiosis / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology
chemical_list: D029681:Arabidopsis Proteins; D010940:Plant Proteins; D017346:Protein Serine-Threonine Kinases; C531469:At3g01090 protein, Arabidopsis
keywords: HSPRO; Nicotiana attenuata; Piriformospora indica; SnRK1; plant growth promotion; signaling