title: Broad-spectrum virucidal activity of (NVC-422) N,N-dichloro-2,2-dimethyltaurine against viral ocular pathogens in vitro.
abstract: OBJECTIVE
  Viral conjunctivitis is a highly contagious infection often causing major epidemics. A safe broad-spectrum antiviral agent is needed to treat this unmet medical need. The purpose of this study is to demonstrate that in vitro NVC-422 is a safe, broad-spectrum topical virucidal agent with activity against ophthalmic viral pathogens.
  
  
  METHODS
  The virucidal activity of NVC-422 against several serotypes of human adenovirus (HAdV), coxsackievirus A24, enterovirus 70, and herpes simplex-virus-1 (HSV-1) was tested in standard in vitro titer reduction assays with or without tears. An in vitro irritancy score for NVC-422 was determined using the MatTek EpiOcular tissue system.
  
  
  RESULTS
  NVC-422 reduced the viral titer of HAdV-5, HAdV-8, HAdV-19, HAdV-37, and HSV-1 by at least 4 logs after 1 hour incubation at 250 μM. Incubation of coxsackievirus A24 and enterovirus 70 with 2.5 mM NVC-422 for 1 hour reduced the viral titer by 4 logs and 4.5 logs, respectively. The virucidal activity of NVC-422 is maintained in the presence of 10% synthetic tears. In the EpiOcular corneal tissue model, NVC-422 was nonirritating at concentrations up to 41 mM.
  
  
  CONCLUSIONS
  NVC-422 has potent, rapid in vitro virucidal activity against major causes of conjunctivitis. Its broad-spectrum virucidal activity combined with favorable safety profile validates NVC-422 as a potential new therapeutic agent against viral conjunctivitis.
authors: NovaBay Pharmaceuticals, Incorporated, Emeryville, California, USA.
authors: Andreas
authors: A
authors: Jekle
authors: Suriani
authors: S
authors: Abdul Rani
authors: Chris
authors: C
authors: Celeri
authors: Meghan
authors: M
authors: Zuck
authors: Ping
authors: P
authors: Xu
authors: Lu
authors: L
authors: Wang
authors: Kathryn
authors: K
authors: Najafi-Tagol
authors: Mark
authors: M
authors: Anderson
authors: David
authors: D
authors: Stroman
authors: Dmitri
authors: D
authors: Debabov
mesh_terms: D000260:Adenoviruses, Human / Q000187:drug effects*; D002478:Cells, Cultured; D003228:Conjunctiva / Q000473:pathology / Q000821:virology*; D003236:Conjunctivitis, Viral / Q000188:drug therapy* / Q000473:pathology / Q000821:virology; D006801:Humans; D013654:Taurine / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D013654:Taurine; C560851:NVC-422