title: Preparation and use of poly(hydroxyethyl methacrylate) cryogels containing L-histidine for β-casein adsorption.
abstract: The objective of this study was to determine β-casein adsorption by using supermacroporous poly(2-hydroxyethyl methacrylate-N-methacryloyl-(l)-histidine methyl ester) [p(HEMA-MAH)] cryogel. β-Casein adsorption properties of p(HEMA-MAH) cryogel were studied for the application of β-casein purification. The cryogel was produced by free radical polymerization initiated by N,N,N',N'-tetramethylene diamine and ammonium persulfate pairs in an ice bath. P(HEMA-MAH) cryogel was characterized by swelling tests, Fourier transform infrared spectroscopy, and scanning electron microscopy. The effects of the flow rate, pH, temperature, initial β-casein concentration, and ionic strength on the adsorption efficiency of cryogel were studied. The equilibrium swelling degree of the p(HEMA-MAH) cryogel was 6.73 g H(2) O/g cryogel. β-Casein adsorption capacity of p(HEMA-MAH) cryogel from aqueous solution was estimated as 31.17 mg/g cryogel. It was also observed that β-casein could be repeatedly adsorbed and desorbed with p(HEMA-MAH) cryogel without significant loss in the adsorption capacity.
authors: Dicle Univ., Faculty of Science, Dept. of Chemistry, 21280 Diyarbakır, Turkey. myavuz@dicle.edu.tr
authors: Murat
authors: M
authors: Yavuz
authors: Zübeyde
authors: Z
authors: Baysal
mesh_terms: D000327:Adsorption; D000645:Ammonium Sulfate / Q000737:chemistry; D002364:Caseins / Q000737:chemistry*; D059985:Cryogels / Q000032:analysis / Q000737:chemistry*; D006639:Histidine / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D009994:Osmolar Concentration; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D058105:Polymerization; D011700:Putrescine / Q000737:chemistry; D013696:Temperature
chemical_list: D002364:Caseins; D059985:Cryogels; C031276:ammonium peroxydisulfate; D011102:Polyhydroxyethyl Methacrylate; D006639:Histidine; C053861:histidine methyl ester; D000645:Ammonium Sulfate; D011700:Putrescine