title: Improved allele-specific PCR technique for Kidd blood group genotyping.
abstract: BACKGROUND
  We developed an allele-specific polymerase chain reaction (AS-PCR) technique for Kidd blood group genotyping.
  
  
  METHODS
  Altogether, 340 blood samples from Thai blood donors at the National Blood Centre, Thai Red Cross Society, were tested with anti-Jk(a) and anti-Jk(b) using the gel technique and the direct urea lysis test was used for screening Jk(a-b-) phenotype. For AS-PCR technique, different types of primers were used for JK*01 and JK*02 allele detections in known DNA controls.
  
  
  RESULTS
  Regarding JK*02 allele detection, the pseudopositve amplification products were found when using correctly matched forward primer and a single mismatch forward primer. Interestingly, one type of two mismatch pairing at the 3' end of the forward primer can be used together with the newly designed reverse primer for Kidd blood group genotyping. It was found that the typing results in all samples obtained by serological techniques and newly developed AS-PCR technique were in agreement and this PCR technique also gave 100% concordance of results in 30 samples randomly tested twice and demonstrated reproducible results.
  
  
  CONCLUSIONS
  This study shows that the in-house AS-PCR is simple, cost-effective, and convenient for Kidd blood group genotyping in routine laboratories, especially, in resolving serologic investigations.
authors: Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University Pathumtani, Thailand.
authors: Kamphon
authors: K
authors: Intharanut
authors: Rudi
authors: R
authors: Grams
authors: Sasitorn
authors: S
authors: Bejrachandra
authors: Pramote
authors: P
authors: Sriwanitchrak
authors: Oytip
authors: O
authors: Nathalang
mesh_terms: D000483:Alleles; D017931:DNA Primers; D005838:Genotype; D006801:Humans; D007667:Kidd Blood-Group System / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013785:Thailand
chemical_list: D017931:DNA Primers; D007667:Kidd Blood-Group System