title: A serine-to-asparagine mutation at position 314 of H5N1 avian influenza virus NP is a temperature-sensitive mutation that interferes with nuclear localization of NP.
abstract: We have generated a temperature-sensitive (ts) mutant from a human isolate of the H5N1 avian influenza virus by classical adaptation in cell culture. After 20 passages at low temperature, the virus showed a ts phenotype. The ts mutant also showed an attenuated phenotype after nasal inoculation in mice. Using reverse genetics, we generated reassortants carrying individual genomic segments of the wild-type and mutant viruses in an A/Puerto Rico/8/34 background, and found that the nucleoprotein (NP) gene could confer the ts phenotype. This mutant NP contains a serine-to-asparagine mutation at position 314 (S314N). The mutant NP protein showed a defect in nuclear localization at high temperature in mammalian cells.
authors: Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Prannok Road, Bangkoknoi, Bangkok 10700, Thailand.
authors: Nattamon
authors: N
authors: Siboonnan
authors: Wittawat
authors: W
authors: Wiriyarat
authors: Chompunuch
authors: C
authors: Boonarkart
authors: Warunya
authors: W
authors: Chakritbudsabong
authors: Anan
authors: A
authors: Jongkaewwattana
authors: Pilaipan
authors: P
authors: Puthavathana
authors: Prasert
authors: P
authors: Auewarakul
authors: Ornpreya
authors: O
authors: Suptawiwat
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics* / Q000502:physiology; D000818:Animals; D001216:Asparagine; D003001:Cloning, Molecular; D005260:Female; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D007251:Influenza, Human / Q000821:virology; D061985:Madin Darby Canine Kidney Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D019590:Nucleocapsid Proteins; D010641:Phenotype; D016601:RNA-Binding Proteins / Q000235:genetics*; D012694:Serine; D013696:Temperature; D014758:Viral Core Proteins / Q000235:genetics*
chemical_list: C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; D016601:RNA-Binding Proteins; D014758:Viral Core Proteins; D012694:Serine; D001216:Asparagine