title: Does dynamic immobilization reduce chondrocyte apoptosis and disturbance to the femoral head perfusion?
abstract: The purpose of this study is to investigate whether the dynamic hip immobilization is more favourable for lessening ischemic injury to the immature femoral head than a static immobilization. 152 Japanese white rabbits were divided into four groups randomly, and the hips were immobilized into "human" position (group A), "frog leg" position (group B) and "dynamic frog leg" position (group C). Group D was used as control. Ten rabbits in each group were killed, and the hip specimens were harvested at 1, 2, and 3 weeks after immobilization. Bcl-2/Bax expression balance and chondrocytes apoptosis were analyzed. The remaining eight rabbits in each group were used to measure the blood supply of capital femoral epiphysis by selective vascular perfusion with Indian ink. The Bcl-2/Bax expression ratio in group C was significantly increased than that in group A and B (p<0.001), while that was not significantly different from control group (p=0.0592). At three weeks after immobilization, the average apoptotic ratio was 36.7%, 45.8%, and 26.7% in group A, B and C, respectively (p<0.01). There was no significant difference between group C and normal control (p=0.0597). The perfusion ratio was 0.03±0.03, 0.03±0.02, and 0.08±0.03 in group A, B and C respectively, and 0.12±0.04 in control group (p<0.05). Thus, the dynamic immobilization model exhibited a relatively less chondrocytes apoptosis and disturbance to the femoral head perfusion than other immobilizations in vivo, which therefore may be useful for reducing avascular necrosis following the treatment of developmental dysplasia of the hip.
authors: Department of Pediatric Orthopedics, Shengjing Hospital of China Medical University Shenyang City, Liaoning Province, PR China.
authors: Lian-Yong
authors: LY
authors: Li
authors: Li-Jun
authors: LJ
authors: Zhang
authors: Jing-Yu
authors: JY
authors: Jia
authors: Qun
authors: Q
authors: Zhao
authors: En-Bo
authors: EB
authors: Wang
authors: Qi-Wei
authors: QW
authors: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002370:Casts, Surgical / Q000009:adverse effects; D019902:Chondrocytes / Q000473:pathology*; D004195:Disease Models, Animal; D004838:Epiphyses / Q000098:blood supply / Q000254:growth & development / Q000378:metabolism; D005269:Femur / Q000098:blood supply / Q000254:growth & development / Q000378:metabolism; D005271:Femur Head Necrosis / Q000473:pathology* / Q000517:prevention & control*; D019417:Hindlimb Suspension / Q000009:adverse effects* / Q000379:methods*; D006618:Hip Dislocation, Congenital / Q000628:therapy; D006621:Hip Joint / Q000098:blood supply / Q000254:growth & development; D007511:Ischemia / Q000473:pathology / Q000517:prevention & control; D011187:Posture; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011817:Rabbits; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein
keywords: Dynamic immobilization; apoptosis; blood supply; femoral head