title: Amplification of complete gag gene sequences from geographically distinct equine infectious anemia virus isolates.
abstract: In the current study, primers described previously and modified versions of these primers were evaluated for amplification of full-length gag genes from different equine infectious anemia virus (EIAV) strains from several countries, including the USA, Germany and Japan. Each strain was inoculated into a primary horse leukocyte culture, and the full-length gag gene was amplified by reverse transcription polymerase chain reaction. Each amplified gag gene was cloned into a plasmid vector for sequencing, and the detectable copy numbers of target DNA were determined. Use of a mixture of two forward primers and one reverse primer in the polymerase chain reaction enabled the amplification of all EIAV strains used in this study. However, further study is required to confirm these primers as universal for all EIAV strains. The nucleotide sequence of gag is considered highly conserved, as evidenced by the use of gag-encoded capsid proteins as a common antigen for the detection of EIAV in serological tests. However, significant sequence variation in the gag genes of different EIAV strains was found in the current study.
authors: School of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors: Bazartseren
authors: B
authors: Boldbaatar
authors: Tsevel
authors: T
authors: Bazartseren
authors: Ryota
authors: R
authors: Koba
authors: Hironobu
authors: H
authors: Murakami
authors: Keisuke
authors: K
authors: Oguma
authors: Kenji
authors: K
authors: Murakami
authors: Hiroshi
authors: H
authors: Sentsui
mesh_terms: D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D004859:Equine Infectious Anemia / Q000175:diagnosis* / Q000821:virology; D005784:Gene Amplification; D015750:Genes, gag; D014644:Genetic Variation; D006736:Horses; D004860:Infectious Anemia Virus, Equine / Q000235:genetics*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA