title: High throughput analysis and capture of benzo[a]pyrene using supermacroporous poly(4-vinyl pyridine-co-divinyl benzene) cryogel matrix.
abstract: A novel supermacroporous cryogel matrix, poly(4-vinyl pyridine-co-divinyl benzene) has been synthesized by free radical polymerization using 4-vinyl pyridine and divinyl benzene as monomers and poly(ethylene glycol) diacrylate as crosslinker. Hydrophobic property of such synthesized matrix allows capture of polyaromatic hydrocarbons like benzo[a]pyrene. The cryogel matrix produced is highly porous (porosity more than 80%) having large and interconnected pores in the range of 10-100 μm that allows easy passage of particulate matter and viscous liquids. The newly synthesized cryogel minicolumns (0.5 ml; 0.9 cm dia.× 1.2 cm height) were used to develop a high throughput analysis system in a miniaturized platform (open ended 96-well microtitre plate). The performance of the cryogel columns in 96-well microtitre plate was reproducible in different wells having approximately 72% binding. This system is drainage- and leakage- protected and can be used for the easy, fast and parallel analysis of many samples. The 96-well format also allows efficient analysis of benzo[a]pyrene in the spiked sample. The cryogel matrix was then used for column chromatography, which showed that the matrix has a binding capacity of 2 μg per ml of matrix for benzo[a]pyrene, thus providing a system for remediation/separation of benzo[a]pyrene. The chromatographic column could bind and partially separate the spiked mixture of benzo[a]pyrene and anthracene.
authors: Department of Biological Sciences and Bioengineering and Centre for Environmental Science and Engineering, Indian Institute of Technology Kanpur, Kanpur 208 016, Uttar Pradesh, India.
authors: Ankur
authors: A
authors: Gupta
authors: Joyita
authors: J
authors: Sarkar
authors: Ashok
authors: A
authors: Kumar
mesh_terms: D001564:Benzo(a)pyrene / Q000032:analysis*; D059985:Cryogels; D008855:Microscopy, Electron, Scanning; D011725:Pyridines / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014753:Vinyl Compounds / Q000737:chemistry*
chemical_list: D059985:Cryogels; D011725:Pyridines; D014753:Vinyl Compounds; C509367:poly(4-vinylpyridine-co-divinylbenzene); D001564:Benzo(a)pyrene