title: Development of a rapid and high-performance chemiluminescence immunoassay based on magnetic particles for protein S100B in human serum.
abstract: Protein S100B is a clinically useful non-invasive biomarker for brain cell damage. A rapid chemiluminescence immunoassay (CLIA) for S100B in human serum has been developed. Fluorescein isothiocyanate (FITC) and N-(aminobutyl)-N-(ethylisoluminol) (ABEI) are used to label two different monoclonal antibodies of anti-S100B. Protein S100B in serum combines with labeled antibodies and can form a sandwiched immunoreaction. A simplified separation procedure based on the use of magnetic particles (MPs) that were coated with anti-FITC antibody is performed to remove the unwanted materials. After adding the substrate solution, the relative light unit (RLU) of ABEI is measured and is found to be directly proportional to the concentration of S100B in serum. The relevant variables involved in the CLIA signals are optimized and the parameters of the proposed method are evaluated. The results demonstrate that the method is linear to 25 ng/mL S100B with a detection limit of 0.02 ng/mL. The coefficient of variation (CV) is < 5% and < 6% for intra- and interassay precision, respectively. The average recoveries are between 97 and 107%. The linearity-dilution effect produces a linear correlation coefficient of 0.9988. Compared with the commercial kit, the proposed method shows a correlation of 0.9897. The proposed method displays acceptable performance for quantification of S100B and is appropriate for use in clinical diagnosis.
authors: National-Reginoal Key Technology Engineering Laboratory for Medical Ultrasound, Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Medical School, Shenzhen University, Guangdong, 518020, People's Republic of China.
authors: Huisheng
authors: H
authors: Zhang
authors: Suwen
authors: S
authors: Qi
authors: Jie
authors: J
authors: Rao
authors: Qiaoliang
authors: Q
authors: Li
authors: Li
authors: L
authors: Yin
authors: Yuejun
authors: Y
authors: Lu
mesh_terms: D006801:Humans; D007118:Immunoassay; D049449:Luminescence; D060328:Magnetic Phenomena; D010316:Particle Size; D064568:S100 Calcium Binding Protein beta Subunit / Q000097:blood*; D013997:Time Factors
chemical_list: D064568:S100 Calcium Binding Protein beta Subunit; C579160:S100B protein, human
keywords: N-(aminobutyl)-N-(ethylisoluminol); chemiluminescence immunoassay; fluorescein-5-isothiocyanate; magnetic particles; protein S100B