title: Fat mass and obesity-associated gene enhances oxidative stress and lipogenesis in nonalcoholic fatty liver disease.
abstract: OBJECTIVE
  Nonalcoholic fatty liver disease (NAFLD) is strongly associated with obesity, hyperlipidemia, and type 2 diabetes mellitus. Several studies have found that fat mass and the obesity-associated (FTO) gene is linked to obesity. The aim of this work is to investigate the expression and function of FTO in liver with lipid metabolism diseases.
  
  
  METHODS
  We investigated the basal FTO expression in an NAFLD rat model and compared it with control subjects. The function of FTO in lipid metabolism was further studied in L02 cells through overexpression experiments.
  
  
  RESULTS
  A significant increase in FTO mRNA and protein levels was found in the NAFLD group. In addition, the FTO levels were positively associated with malondialdehyde and superoxide dismutase concentrations. FTO overexpression in L02 cells enhanced lipogenesis and oxidative stress.
  
  
  CONCLUSIONS
  This study demonstrates that increased FTO levels in the liver are involved in oxidative stress and lipid deposition, which characterize NAFLD.
authors: Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu 210029, China.
authors: Jianjin
authors: J
authors: Guo
authors: Wei
authors: W
authors: Ren
authors: Aimei
authors: A
authors: Li
authors: Ying
authors: Y
authors: Ding
authors: Wanhua
authors: W
authors: Guo
authors: Dongming
authors: D
authors: Su
authors: Cheng
authors: C
authors: Hu
authors: Kuanfeng
authors: K
authors: Xu
authors: Heng
authors: H
authors: Chen
authors: Xinyu
authors: X
authors: Xu
authors: Tao
authors: T
authors: Yang
authors: Weiping
authors: W
authors: Jia
mesh_terms: D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO; D000818:Animals; D001835:Body Weight; D059305:Diet, High-Fat; D004041:Dietary Fats / Q000494:pharmacology*; D005234:Fatty Liver / Q000097:blood / Q000235:genetics* / Q000473:pathology; D050155:Lipogenesis / Q000235:genetics*; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D009929:Organ Size; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004041:Dietary Fats; D011506:Proteins; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO; C529321:FTO protein, rat