title: Cyt1Aa from Bacillus thuringiensis subsp. israelensis enhances mosquitocidal activity of B. thuringiensis subsp. kurstaki HD-1 against Aedes aegypti but not Culex quinquefasciatus.
abstract: The Cyt1Aa protein of Bacillus thuringiensis subsp. israelensis is known to synergize mosquitocidal proteins of B. thuringiensis and Bacillus sphaericus strains. Cyt1Aa is highly lipophilic, and after binding in vivo to the midgut microvillar membrane serves as a "receptor" for mosquitocidal Cry proteins, which subsequently form cation channels that kill mosquito larvae. Here we report that Cyt1Aa can serve a similar function for lepidopteran-specific Cry proteins of B. thuringiensis in certain mosquito larvae. Engineering Cyt1Aa into the HD-1 isolate of B. thuringiensis subsp. kurstaki enhanced toxicity against 4th instars of Aedes aegypti, but not against 4th instars of Culex quinquefasciatus.
authors: Department of Natural and Mathematical Sciences, California Baptist University, Riverside, CA 92504, USA.
authors: Hyun-Woo
authors: HW
authors: Park
authors: Brent C
authors: BC
authors: Pino
authors: Switzerlyna
authors: S
authors: Kozervanich-Chong
authors: Erika A
authors: EA
authors: Hafkenscheid
authors: Ryan M
authors: RM
authors: Oliverio
authors: Brian A
authors: BA
authors: Federici
authors: Dennis K
authors: DK
authors: Bideshi
mesh_terms: D000330:Aedes / Q000187:drug effects / Q000382:microbiology / Q000502:physiology*; D000818:Animals; D001413:Bacillus thuringiensis / Q000472:pathogenicity*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000494:pharmacology*; D003465:Culex / Q000187:drug effects / Q000382:microbiology / Q000502:physiology*; D004731:Endotoxins / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000494:pharmacology*; D007306:Insecticides / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000382:microbiology / Q000502:physiology; D016019:Survival Analysis
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis