title: Formin homology 1 (OsFH1) regulates root-hair elongation in rice (Oryza sativa).
abstract: The outgrowth of root hairs from the epidermal cell layer is regulated by a strict genetic regulatory system and external growth conditions. Rice plants cultivated in water-logged paddy land are exposed to a soil ecology that differs from the environment surrounding upland plants, such as Arabidopsis and maize. To identify genes that play important roles in root-hair growth, a forward genetics approach was used to screen for short-root-hair mutants. A short-root-hair mutant was identified, and the gene was isolated using map-based cloning and sequencing. The mutant harbored a point mutation at a splicing acceptor site, which led to truncation of OsFH1 (rice formin homology 1). Subsequent analysis of two additional T-DNA mutants verified that OsFH1 is important for root-hair elongation. Further studies revealed that the action of OsFH1 on root-hair growth is dependent on growth conditions. The mutant Osfh1 exhibited root-hair defects when roots were grown submerged in solution, and mutant roots produced normal root hairs in the air. However, root-hair phenotypes of mutants were not influenced by the external supply of hormones or carbohydrates, a deficiency of nutrients, such as Fe or P i , or aeration. This study shows that OsFH1 plays a significant role in root-hair elongation in a growth condition-dependent manner.
authors: Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju, 660-701, Korea.
authors: Jin
authors: J
authors: Huang
authors: Chul Min
authors: CM
authors: Kim
authors: Yuan-hu
authors: YH
authors: Xuan
authors: Jingmiao
authors: J
authors: Liu
authors: Tae Ho
authors: TH
authors: Kim
authors: Bo-Kyeong
authors: BK
authors: Kim
authors: Chang-deok
authors: CD
authors: Han
mesh_terms: D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D012275:Oryza / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D010940:Plant Proteins