title: Three antimycobacterial metabolites identified from a marine-derived Streptomyces sp. MS100061.
abstract: In the course of a screening program for bioactive compounds from a marine natural product library, a newly isolated Actinomycetes strain, designated as MS100061, exhibited strong anti-Mycobacterium bovis Bacillus Calmette-Guérin (BCG) activity. The strain belongs to the genus Streptomyces according to its morphological and 16S rDNA phylogenetic analysis. Bioassay-guided isolation resulted in a new spirotetronate, lobophorin G (1), together with two known compounds, lobophorins A (2) and B (3). The structures were elucidated by extensive spectroscopic methods and comparison with literatures. Compounds 1-3 were subjected to anti-BCG, antituberculosis, and antibacterial screening and exhibited potent anti-BCG activity with minimum inhibitory concentration (MIC) values of 1.56, 1.56, and 0.78 μg/ml, respectively, and moderate anti-Mycobacterium tuberculosis H37Rv activity with MIC values of 32, 32, and 16 μg/ml, respectively. The MIC values of compounds 1-3 against Bacillus subtilis were 3.125, 12.5, and 1.56 μg/ml, respectively, indicating great potential for antibacterial drugs. In addition, this is the first report of the anti-BCG and antituberculosis activities of lobophorins.
authors: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.
authors: Caixia
authors: C
authors: Chen
authors: Jian
authors: J
authors: Wang
authors: Hui
authors: H
authors: Guo
authors: Weiyuan
authors: W
authors: Hou
authors: Na
authors: N
authors: Yang
authors: Biao
authors: B
authors: Ren
authors: Mei
authors: M
authors: Liu
authors: Huanqin
authors: H
authors: Dai
authors: Xueting
authors: X
authors: Liu
authors: Fuhang
authors: F
authors: Song
authors: Lixin
authors: L
authors: Zhang
mesh_terms: D039903:Actinobacteria / Q000737:chemistry; D000995:Antitubercular Agents / Q000737:chemistry* / Q000494:pharmacology*; D018942:Macrolides / Q000737:chemistry / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D009163:Mycobacterium bovis / Q000187:drug effects*; D013302:Streptomyces / Q000737:chemistry*
chemical_list: D000995:Antitubercular Agents; D018942:Macrolides; C121297:lobophorin A; C121298:lobophorin B