title: Potent growth-inhibitory effect of a dual cancer-specific oncolytic             adenovirus expressing apoptin on prostate carcinoma.
abstract: Apoptin is a chicken anemia virus-derived, p53-independent, bcl-2-insensitive apoptotic protein with the ability to specifically induce apoptosis in various human tumor cells, but not in normal cells. To explore the use of apoptin in tumor gene therapy, we assessed a recombinant adenovirus expressing the apoptin protein (Ad-hTERTp-E1a-Apoptin) in order to determine its lethal and growth-inhibitory effects on PC-3 and RM-1 cells in vitro and its antitumor effect on solid tumors in vivo. 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), acridine orange (AO)/ethidium bromide (EB), 4'-6-diamidino-2-phenylindole (DAPI), and Annexin V assays showed that Ad-hTERTp-E1a-Apoptin inhibited the proliferation of PC-3 and RM-1 cells in vitro by inducing apoptosis of prostate cancer cells, and that this inhibitory effect was dose and time-dependent. In the animal models, Ad-hTERTp-E1a-Apoptin significantly inhibited tumor growth and extended the lifespan of animals. Experimental results indicate that Ad-hTERTp-E1a-Apoptin has a potential application in tumor gene therapy.
authors: Department of Urology, China-Japan Union Hospital of Jilin University,                     Changchun 130031, P.R. China.
authors: Muchun
authors: M
authors: Zhang
authors: Jinhui
authors: J
authors: Wang
authors: Chang
authors: C
authors: Li
authors: Ningning
authors: N
authors: Hu
authors: Kai
authors: K
authors: Wang
authors: Huifan
authors: H
authors: Ji
authors: Dongyun
authors: D
authors: He
authors: Chengshi
authors: C
authors: Quan
authors: Xiao
authors: X
authors: Li
authors: Ningyi
authors: N
authors: Jin
authors: Yulin
authors: Y
authors: Li
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D017727:Chicken anemia virus / Q000235:genetics / Q000378:metabolism; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D050504:Oncolytic Viruses / Q000235:genetics; D011401:Promoter Regions, Genetic; D011471:Prostatic Neoplasms / Q000628:therapy*; D011897:Random Allocation
chemical_list: D051017:Apoptosis Regulatory Proteins; D036022:Capsid Proteins; C091717:VP3 protein, Chicken anemia virus