title: Proteomic analysis of Herbaspirillum seropedicae cultivated in the presence of sugar cane extract.
abstract: Bacterial endophytes of the genus Herbaspirillum colonize sugar cane and can promote plant growth. The molecular mechanisms that mediate plant- H. seropedicae interaction are poorly understood. In this work, we used 2D-PAGE electrophoresis to identify H. seropedicae proteins differentially expressed at the log growth phase in the presence of sugar cane extract. The differentially expressed proteins were validated by RT qPCR. A total of 16 differential spots (1 exclusively expressed, 7 absent, 5 up- and 3 down-regulated) in the presence of 5% sugar cane extract were identified; thus the host extract is able to induce and repress specific genes of H. seropedicae. The differentially expressed proteins suggest that exposure to sugar cane extract induced metabolic changes and adaptations in H. seropedicae presumably in preparation to establish interaction with the plant.
authors: Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná, Centro Politécnico, PO Box 19071, Curitiba, PR 81531-990, Brazil.
authors: Fabio Aparecido
authors: FA
authors: Cordeiro
authors: Michelle Zibetti
authors: MZ
authors: Tadra-Sfeir
authors: Luciano Fernandes
authors: LF
authors: Huergo
authors: Fábio
authors: F
authors: de Oliveira Pedrosa
authors: Rose Adele
authors: RA
authors: Monteiro
authors: Emanuel Maltempi
authors: EM
authors: de Souza
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D042803:Herbaspirillum / Q000378:metabolism*; D008969:Molecular Sequence Data; D010936:Plant Extracts / Q000008:administration & dosage*; D040901:Proteomics; D060888:Real-Time Polymerase Chain Reaction; D031786:Saccharum / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D010936:Plant Extracts