title: [Application of secretary luciferase labeled orthotopic transplant model of hepatocellular carcinoma to evaluate tumor response to interferon-beta gene therapy].
abstract: To establish an orthotopic transplant mouse model of hepatocellular carcinoma (HCC) labeled with secretary luciferase and to study its response to anti-tumor treatment with interferon-beta gene therapy. We labeled the murine hepatoma Hepal-6 cells with secretary Gaussia princeps luciferase (Gluc), and then injected Gluc labeled Hepal-6 cells intrasplenically in C57BL/6 mice. We monitored blood Glue to evaluate the tumor development and anti-tumor effects of hydrodynamic injection with interferon-beta expressing plasmid. We successfully established the orthotopic mouse model of HCC by intrasplenic injection of Glue labeled Hepal-6 cells. The Glue blood assay could reflect the amount of cancer cells in vivo, tumor progression, as well as anti-tumor effect of interferon-beta gene therapy. In conclusion, Gluc labeled orthotopic transplant mouse model of HCC can ex vivo real-time monitor the tumor development and tumor response to treatments.
authors: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors: Gang
authors: G
authors: Wang
authors: Zhonghui
authors: Z
authors: Lian
authors: Wenhong
authors: W
authors: Tian
authors: Xiaoyan
authors: X
authors: Dong
authors: Jie
authors: J
authors: Yuchi
authors: Xiaobing
authors: X
authors: Wu
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D017930:Genes, Reporter; D015316:Genetic Therapy; D016899:Interferon-beta / Q000235:genetics* / Q000627:therapeutic use*; D008113:Liver Neoplasms / Q000473:pathology / Q000628:therapy*; D008156:Luciferases / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D016896:Treatment Outcome
chemical_list: D016899:Interferon-beta; D008156:Luciferases