title: Effects of temporary vascular occluder poloxamer 407 gel on the endothelium.
abstract: BACKGROUND
  Coronary occlusion techniques during OPCAB may lead to an endothelial damage to the target vessel. The adverse effects of these techniques are well-known, and researches have been trying to find out new materials to occlude the coronary artery without an endothelial damage. In the present study, we investigate to the endothelial damage in the rat aorta which is occluded by Poloxamer 407 gel.
  
  
  METHODS
  Forty-five rats were randomized in three groups: (1) segment of the aorta was occluded with Poloxamer 407 gel in P 407 group; (2) segment of the aorta was occluded with microvascular clamp in MV clamp group; and (3) no onclusion was available in the Control group. The rats were sacrificed of observation, and a 15mm segment of the aorta was obtained as a specimen. Integrity of the endothelial lining was observed with a scanning electron microscopy.
  
  
  RESULTS
  Scanning electron microscopy revealed a statistically significant difference among the 3 groups (p<0,001) using the SPSS 13.0 test. No difference was found between the Control group and the P 407 group (p=0,059). The differences between MV clamp-Control group (p<0,001) and MV clamp-P 407 group were statistically significant (p<0,002).
  
  
  CONCLUSIONS
  We suggest that Poloxamer 407 gel occlusion may be a safer and more effective method compared to the microvascular clamp occlusion.
authors: Department of Cardiovascular Surgery, Bursa Yuksek Ihtisas Training and Research Hospital, Bursa, Turkey. dr.arifgucu@gmail.com
authors: Arif
authors: A
authors: Gucu
authors: Ilkin
authors: I
authors: Cavusoglu
authors: Onder
authors: O
authors: Bozkurt
authors: Cuneyt
authors: C
authors: Eris
authors: Faruk
authors: F
authors: Toktas
authors: Tugrul
authors: T
authors: Goncu
authors: Ahmet
authors: A
authors: Ozyazicioglu
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology / Q000187:drug effects / Q000473:pathology; D054059:Coronary Occlusion / Q000473:pathology; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D005260:Female; D006489:Hemostatic Techniques; D008855:Microscopy, Electron, Scanning; D020442:Poloxamer / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric
chemical_list: D020442:Poloxamer