title: Functional activity of neutrophils in diabetes mellitus and coronary heart disease: role of myeloperoxidase in the development of oxidative stress.
abstract: We performed a comparative analysis of functional activity of neutrophils in patients with type 2 diabetes mellitus with and without symptoms of CHD. Enhanced H2O2 production by neutrophils in response to N-formyl-Met-Leu-Phe (fMLP) was found in patients with type 2 diabetes mellitus. In patients with type 2 diabetes mellitus associated with CHD, fMLP-induced release of myeloperoxidase from azurophilic granules of neutrophils was reduced and plasma myeloperoxidase level was elevated. Increased peroxidase activity of myeloperoxidase, reduced plasma catalase activity, and increased levels of TBA-reactive lipid peroxidation products and oxidized glutathione were detected in patients of both groups. Since myeloperoxidase is an important neutrophilic mediator of oxidative stress, its increased activity in the blood can be an additional marker of oxidative stress and cardiovascular risk in patients with diabetes mellitus.
authors: Belarusian State University, Minsk, Repablic of Belarussia. irinagorudko@rambler.ru
authors: I V
authors: IV
authors: Gorudko
authors: V A
authors: VA
authors: Kostevich
authors: A V
authors: AV
authors: Sokolov
authors: E V
authors: EV
authors: Shamova
authors: I V
authors: IV
authors: Buko
authors: E E
authors: EE
authors: Konstantinova
authors: V B
authors: VB
authors: Vasiliev
authors: S N
authors: SN
authors: Cherenkevich
authors: O M
authors: OM
authors: Panasenko
mesh_terms: D002374:Catalase / Q000378:metabolism; D003327:Coronary Disease / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D004789:Enzyme Activation; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008875:Middle Aged; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D009504:Neutrophils / Q000378:metabolism*; D018384:Oxidative Stress; D009195:Peroxidase / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D009240:N-Formylmethionine Leucyl-Phenylalanine; C023976:N-formylmethionyl-methionyl-phenylalanine; D006861:Hydrogen Peroxide; D002374:Catalase; D009195:Peroxidase; D005978:Glutathione