title: Synthesis and characterization of oligonucleotide conjugates bearing electroactive labels.
abstract: Oxime bond formation has been applied to the preparation of oligonucleotides labeled with electrochemical ferrocene and viologen labels. Aminooxy functionalized ferrocene and viologen derivatives were prepared by a straightforward route and efficiently conjugated with aldehyde containing oligonucleotides either at 3' or 5' end. Both labels were found to not disturb the recognition properties of the oligonucleotide. The versatility of the method was further demonstrated by preparing bi-functionalized conjugates with a disulfide at 3' end and an electrochemical label at 5' end.
authors: Institut de Topologie et de Dynamique des Systèmes, UMR 7086 CNRS/Université Paris Diderot, Sorbonne Paris Cité, 15 rue Jean-Antoine de Baïf, 75205 Paris Cedex, France.
authors: Julie
authors: J
authors: Moreau
authors: Nabil
authors: N
authors: Dendane
authors: Bernd
authors: B
authors: Schöllhorn
authors: Nicolas
authors: N
authors: Spinelli
authors: Claire
authors: C
authors: Fave
authors: Eric
authors: E
authors: Defrancq
mesh_terms: D055664:Electrochemical Techniques / Q000379:methods; D005296:Ferrous Compounds / Q000737:chemistry; D000075163:Metallocenes; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D010091:Oximes / Q000138:chemical synthesis / Q000737:chemistry*; D014755:Viologens / Q000737:chemistry
chemical_list: D005296:Ferrous Compounds; D000075163:Metallocenes; D009841:Oligonucleotides; D010091:Oximes; D014755:Viologens; C004998:ferrocene