title: c-Fos activity mapping reveals differential effects of noradrenaline and serotonin depletion on the regulation of ocular dominance plasticity in rats.
abstract: The roles of the central noradrenergic and serotonergic system in the activity-dependent regulation of ocular dominance plasticity have been a contentious issue. Using c-Fos activity mapping, we have developed a new, straightforward method to measure the strength of ocular dominance plasticity: the number of c-Fos-immunopositive cells in layer IV of rat visual cortex (Oc1B), ipsilateral to the stimulated eye, is a sensitive and reliable measure of the effects of monocular deprivation. Applying this new method, here we studied the unique modification of the degree of c-Fos expression induced in the visual cortex, in that endogenous noradrenaline (NA) and serotonin (5HT) in the cortex were significantly reduced, respectively by specific pharmacological agents. Intraperitoneal injections of N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP4) and p-chlorophenylalanine (pCPA) selectively impair NA- and 5HT-containing nerve terminals and fibers, respectively. In the visual cortex with strongly reduced NA, the number of c-Fos-immunopositive cells was found remaining significantly decreased in response to stimulation of the deprived eye, while by open eye stimulation the expected increase in c-Fos-immunoreactivity was strongly suppressed, showing values not different from those obtained by monocular stimulation in the normal rats. In contrast, in the visual cortex with strongly reduced 5HT no expected decrease was found in response to stimulation of the deprived eye, while, as is usually the case for the normal animals, a significant increase was still induced in response to open eye stimulation. These findings suggest that the noradrenergic and serotonergic system regulate ocular dominance (OD) plasticity differently: in the NA-depleted cortex the expected increase in c-Fos expression by open eye stimulation was not seen due to strong suppression, whereas in 5HT-depletion, the expected decrease in c-Fos expression was not materialized due to strong suppression. The present findings with c-Fos activity mapping method indicated a novel possibility of the differential regulation of OD plasticity by two types of common monoaminergic systems.
authors: Department of Basic Biology, Educational and Research Center for Pharmacy, Meiji Pharmaceutical University, Kiyose-shi, Tokyo 204-8588, Japan.
authors: K
authors: K
authors: Nakadate
authors: K
authors: K
authors: Imamura
authors: Y
authors: Y
authors: Watanabe
mesh_terms: D000818:Animals; D001596:Benzylamines / Q000494:pharmacology; D002540:Cerebral Cortex / Q000502:physiology; D002874:Chromosome Mapping; D023882:Dominance, Ocular / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D010134:Fenclonine / Q000494:pharmacology; D007150:Immunohistochemistry; D008297:Male; D009473:Neuronal Plasticity / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D014179:Neurotransmitter Uptake Inhibitors; D009638:Norepinephrine / Q000502:physiology*; D010775:Photic Stimulation; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000502:physiology*; D051381:Rats; D020318:Rats, Long-Evans; D012683:Sensory Deprivation; D012701:Serotonin / Q000502:physiology*; D018490:Serotonin Agents / Q000494:pharmacology; D015349:Vision, Monocular; D014793:Visual Cortex / Q000502:physiology
chemical_list: D001596:Benzylamines; D014179:Neurotransmitter Uptake Inhibitors; D016760:Proto-Oncogene Proteins c-fos; D018490:Serotonin Agents; D012701:Serotonin; C012102:DSP 4; D010134:Fenclonine; D009638:Norepinephrine