title: Matrix effects in the determination of β-receptor agonists in animal-derived foodstuffs by ultra-performance liquid chromatography tandem mass spectrometry with immunoaffinity solid-phase extraction.
abstract: Matrix effects in determination of three β-receptor agonists including salbutamol (SAL), clenbuterol, and terbutaline in animal-derived foodstuffs were studied by ultra-performance LC-MS/MS with cleanup of immunoaffinity SPE column (IAC). Some animal tissue samples including pig liver, swine muscle, and fish muscle were hydrolyzed by the mixed enzyme solution or HCl solution, and the cleanup efficiencies with SAL IAC, MCX SPE column, and C(18) -SCX tandem columns were examined and compared by using spiked experiments. The results showed that the matrix effects in the determination of SAL and terbutaline can be eliminated with SAL IAC cleanup, and the average recoveries of SAL were 77.4~81.5%, 79.0~80.3%, and 85.0~87.2% in pig liver, swine muscle, and fish muscle, respectively. The decision limit (ccα) and detection capability (ccβ) for SAL in pig liver were 0.02 and 0.05 μg/kg, respectively.
authors: Chongqing Entry-Exit Inspection and Quarantine Bureau, Chongqing Engineering Technology Research Center for Import and Export Food Safety, Chongqing, China.
authors: Guomin
authors: G
authors: Wang
authors: Jian
authors: J
authors: Zhao
authors: Tao
authors: T
authors: Peng
authors: Dongdong
authors: D
authors: Chen
authors: Cunxian
authors: C
authors: Xi
authors: Xiong
authors: X
authors: Wang
authors: Jinzhong
authors: J
authors: Zhang
mesh_terms: D000318:Adrenergic beta-Agonists / Q000032:analysis* / Q000302:isolation & purification; D000420:Albuterol / Q000032:analysis* / Q000302:isolation & purification; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005399:Fishes; D005506:Food Contamination; D008099:Liver / Q000737:chemistry; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000318:Adrenergic beta-Agonists; D000420:Albuterol