title: Ultrasound-mediated targeted drug delivery generated by multifocal beam patterns: an in vitro study.
abstract: Ultrasound-mediated targeted drug delivery has been a subject for a dedicated research activity for several decades. Nevertheless, in vitro studies in this field of research are characterized by their inconsistencies. To improve the repeatability of such experiments, a novel approach of multifocal spot generation was investigated. A multifocal pattern of 16 spots was utilized using an iterative Gerchberg-Saxton algorithm. The pattern was applied to insonate a 96-well Petri dish plate using a clinically available planar-phased array transducer with approximately 1000 elements with a central frequency of 0.55 MHz. The pattern was acoustically characterized and applied to a monolayer of human breast cancer cell line in the 96-well plate. With the help of ultrasonic contrast agents, the intracellular drug uptake was increased by an average factor of 3.5 compared with the control group.
authors: Institute for Medical Science and Technology, University of Dundee, Dundee DD2 1FD,UK.
authors: Dana
authors: D
authors: Gourevich
authors: Yoni
authors: Y
authors: Hertzberg
authors: Alexander
authors: A
authors: Volovick
authors: Yaron
authors: Y
authors: Shafran
authors: Gil
authors: G
authors: Navon
authors: Sandy
authors: S
authors: Cochran
authors: Andreas
authors: A
authors: Melzer
mesh_terms: D000465:Algorithms; D000935:Antifungal Agents / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000008:administration & dosage; D004317:Doxorubicin / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D045423:Microbubbles; D010743:Phospholipids / Q000008:administration & dosage; D015203:Reproducibility of Results; D013459:Sulfur Hexafluoride / Q000008:administration & dosage; D014159:Transducers; D014465:Ultrasonics / Q000379:methods*
chemical_list: D000935:Antifungal Agents; D003287:Contrast Media; D010743:Phospholipids; C420843:contrast agent BR1; D004317:Doxorubicin; D013459:Sulfur Hexafluoride