title: Modification of proportion sensitivity testing method for ethionamide.
abstract: Standardized methodology for drug susceptibility testing of second line drugs is vital for treatment of multi/extensively drug resistant tuberculosis. Discrepancy between laboratory methods and clinical interpretation is well established for bacteriostatic drugs such as ethionamide. Optimization of the standard proportion sensitivity testing (PST) method for ethionamide was under taken in 235 Mycobacterium tuberculosis isolates from new and previously treated pulmonary tuberculosis patients. An additional higher concentration of 57 μg/ml was evaluated against at the standard 40 μg/ml concentration in PST method. Performance parameters and agreement between the two drug concentrations was higher indicating the efficiency of PST method at its present format at 40 μg/ml and additional higher concentration of 57 μg/ml as an alternative when required.
authors: Department of Bacteriology, National Institute for Research in Tuberculosis (Formerly Tuberculosis Research Centre), Chetpet, Chennai 600 031, India.
authors: Rajagopalan
authors: R
authors: Lakshmi
authors: Ranjani
authors: R
authors: Ramachandran
authors: K
authors: K
authors: Devika
authors: G
authors: G
authors: Radhika
authors: A
authors: A
authors: Syam Sundar
authors: Fathima
authors: F
authors: Rahman
authors: N
authors: N
authors: Selvakumar
authors: Vanaja
authors: V
authors: Kumar
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D005000:Ethionamide / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification; D018088:Tuberculosis, Multidrug-Resistant / Q000382:microbiology*
chemical_list: D000995:Antitubercular Agents; D005000:Ethionamide