title: Optimization of hydrolysis conditions of Palmaria palmata to enhance R-phycoerythrin extraction.
abstract: In this study, response surface methodology was applied to optimize R-phycoerythrin extraction from the red seaweed Palmaria palmata, using enzymatic digestion. Several algal treatments prior to digestion were first investigated. The extraction yield and the purity index of R-phycoerythrin, and the recovery of proteins and reducing sugars in the water-soluble fraction were then studied in relation to the hydrolysis time, the temperature and the enzyme/seaweed ratio. Enzymatic digestion appears to be an effective treatment for R-phycoerythrin extraction. Moreover, using the seaweed roughly cut in its wet form gives the most interesting results in terms of extract quality and economic cost. The R-phycoerythrin extraction yield is 62 times greater than without enzyme treatment and 16 times greater than without optimization. Enzymatic optimization enhanced the purity index up to 16 times.
authors: LUNAM Université, Université de Nantes, MMS, Nantes, 2 rue de la Houssinière, BP 92208, 44322 Nantes Cedex 3, France. justine.dumay@univ-nantes.fr
authors: Justine
authors: J
authors: Dumay
authors: Nathalie
authors: N
authors: Clément
authors: Michèle
authors: M
authors: Morançais
authors: Joël
authors: J
authors: Fleurence
mesh_terms: D002458:Cell Fractionation / Q000379:methods*; D003198:Computer Simulation; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry*; D006868:Hydrolysis; D059625:Liquid-Liquid Extraction / Q000379:methods*; D008954:Models, Biological; D008956:Models, Chemical; D010799:Phycoerythrin / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D000461:Rhodophyta / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D010799:Phycoerythrin; D043364:Endo-1,4-beta Xylanases