title: Loss of alveolar membrane diffusing capacity and pulmonary capillary blood volume in pulmonary arterial hypertension.
abstract: BACKGROUND
  Reduced gas transfer in patients with pulmonary arterial hypertension (PAH) is traditionally attributed to remodeling and progressive loss of pulmonary arterial vasculature that results in decreased capillary blood volume available for gas exchange.
  
  
  METHODS
  We tested this hypothesis by determination of lung diffusing capacity (DL) and its components, the alveolar capillary membrane diffusing capacity (D(m)) and lung capillary blood volume (V(c)) in 28 individuals with PAH in comparison to 41 healthy individuals, and in 19 PAH patients over time. Using single breath simultaneous measure of diffusion of carbon monoxide (DL(CO)) and nitric oxide (DL(NO)), DL and D(m) were respectively determined, and V(c) calculated. D(m) and V(c) were evaluated over time in relation to standard clinical indicators of disease severity, including brain natriuretic peptide (BNP), 6-minute walk distance (6MWD) and right ventricular systolic pressure (RVSP) by echocardiography.
  
  
  RESULTS
  Both DL(CO) and DL(NO) were reduced in PAH as compared to controls and the lower DL in PAH was due to loss of both D(m) and V(c) (all p < 0.01). While DL(CO) of PAH patients did not change over time, DL(NO) decreased by 24 ml/min/mmHg/year (p = 0.01). Consequently, D(m) decreased and V(c) tended to increase over time, which led to deterioration of the D(m)/V(c) ratio, a measure of alveolar-capillary membrane functional efficiency without changes in clinical markers.
  
  
  CONCLUSIONS
  The findings indicate that lower than normal gas transfer in PAH is due to loss of both D(m) and V(c), but that deterioration of D(m)/V(c) over time is related to worsening membrane diffusion.
authors: Respiratory Institute, Cleveland Clinic, Cleveland, OH 44195, USA. farhas@ccf.org
authors: Samar
authors: S
authors: Farha
authors: Daniel
authors: D
authors: Laskowski
authors: Deepa
authors: D
authors: George
authors: Margaret M
authors: MM
authors: Park
authors: W H Wilson
authors: WH
authors: Tang
authors: Raed A
authors: RA
authors: Dweik
authors: Serpil C
authors: SC
authors: Erzurum
mesh_terms: D000328:Adult; D001810:Blood Volume; D002196:Capillaries / Q000503:physiopathology*; D004058:Diffusion; D065627:Familial Primary Pulmonary Hypertension; D005260:Female; D006801:Humans; D006976:Hypertension, Pulmonary / Q000503:physiopathology*; D008297:Male; D011650:Pulmonary Alveoli / Q000737:chemistry / Q000503:physiopathology*; D011653:Pulmonary Diffusing Capacity