title: The association of seminal plasma antioxidant levels and sperm chromatin status with genetic variants of GSTM1 and GSTP1 (Ile105Val and Ala114Val) in infertile men with oligoasthenoteratozoospermia.
abstract: In this study we aimed to examine the effects of genetic variants of GSTM1 and GSTP1 (Ile105Val and Ala114Val) on GST activity, seminal oxidative stress and sperm chromatin status in infertile men with oligoasthenoteratozoospermia (OAT). The study population (n=121) consisted of 95 infertile men with OAT and 26 controls with normozoospermia. Multiplex polymerase chain reaction (PCR) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods were utilized to detect the aforesaid genetic variants. We measured GST activity and total antioxidant capacity (TAC) of seminal plasma by spectrophotometry. Sperm chromatin integrity and maturity were assessed using toluidine blue and chromomycin A3 (CMA3-positive sperm) staining, respectively. The analysis showed that subgroups of GSTM1 null and GSTP1 C/T+T/T genotypes in comparison with GSTM1 present and GSTP1 wild type (C/C) genotypes did not have statistically significant differences in both OAT or normozoospermic men considering sperm concentration and motility, percentage of CMA3-positive sperm, seminal plasma TAC, sperm chromatin integrity and GST activity. Thus, the findings of our study suggest that there are no significant associations between GSTM1 and GSTP1 polymorphisms and sperm parameters at conventional or at molecular levels including OS status, sperm chromatin integrity or maturity in Iranian infertile men with OAT and normozoospermia. However, these polymorphisms could be related to the fertility status of the studied population but not evaluated in this study.
authors: Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
authors: Niknam
authors: N
authors: Lakpour
authors: Azadeh
authors: A
authors: Mirfeizollahi
authors: Shirin
authors: S
authors: Farivar
authors: Mohammad Mehdi
authors: MM
authors: Akhondi
authors: S Behnam
authors: SB
authors: Hashemi
authors: Naser
authors: N
authors: Amirjannati
authors: Hamed
authors: H
authors: Heidari-Vala
authors: Mohammad Reza
authors: MR
authors: Sadeghi
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D053627:Asthenozoospermia / Q000097:blood / Q000235:genetics*; D016022:Case-Control Studies; D002843:Chromatin / Q000235:genetics*; D004247:DNA / Q000032:analysis / Q000235:genetics; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000097:blood / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012661:Semen / Q000737:chemistry; D013094:Spermatozoa / Q000737:chemistry
chemical_list: D000975:Antioxidants; D002843:Chromatin; D004247:DNA; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; C496556:GSTP1 protein, human