title: Effects of bioactive glass S53P4 or beta-tricalcium phosphate and bone morphogenetic protein-2 and bone morphogenetic protein-7 on osteogenic differentiation of human adipose stem cells.
abstract: The effects of bioactive glass S53P4 or beta-tricalcium phosphate; and bone morphogenetic proteins bone morphogenetic protein-2, bone morphogenetic protein-7, or bone morphogenetic protein-2 + 7 on osteogenic differentiation of human adipose stem cells were compared in control medium, osteogenic medium, and bone morphogenetic protein-supplemented osteogenic medium to assess suitability for bone tissue engineering. Cell amount was evaluated with qDNA measurements; osteogenic differentiation using marker gene expression, alkaline phosphate activity, and angiogenic potential was measured by vascular endothelial growth factor expression. As compared to beta-tricalcium phosphate, cell amount was significantly greater for bioactive glass in control medium after 7 days and in osteogenic medium after 14 days, and alkaline phosphate activity was always significantly greater for bioactive glass in control medium. However, alkaline phosphate activity increased for beta-tricalcium phosphate and decreased for bioactive glass granules in osteogenic medium. For both biomaterials, bone morphogenetic protein supplementation decreased cell amount and osteogenic differentiation of human adipose stem cells, and vascular endothelial growth factor expressions correlated with cell amounts. Effects of culture medium on human adipose stem cells are biomaterial dependent; bioactive glass in control medium enhanced osteogenic differentiation most effectively.
authors: Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland ; Adult Stem Cell Group, Institute of Biomedical Technology, University of Tampere, Tampere, Finland.
authors: Martin
authors: M
authors: Waselau
authors: Mimmi
authors: M
authors: Patrikoski
authors: Miia
authors: M
authors: Juntunen
authors: Kasperi
authors: K
authors: Kujala
authors: Minna
authors: M
authors: Kääriäinen
authors: Hannu
authors: H
authors: Kuokkanen
authors: George K
authors: GK
authors: Sándor
authors: Outi
authors: O
authors: Vapaavuori
authors: Riitta
authors: R
authors: Suuronen
authors: Bettina
authors: B
authors: Mannerström
authors: Brigitte
authors: B
authors: von Rechenberg
authors: Susanna
authors: S
authors: Miettinen
keywords: beta-tricalcium phosphate; bioactive glass; bone morphogenetic protein-2; bone morphogenetic protein-7; human adipose stem cells; in vitro; osteogenic differentiation