title: Gold nanoparticles cellular toxicity and recovery: adipose Derived Stromal cells.
abstract: Gold nanoparticles (AuNPs) are currently used in numerous medical applications. Herein, we describe their in vitro impact on human adipose-derived stromal cells (ADSCs) using 13 nm and 45 nm citrate-coated AuNPs. In their non-differentiated state, ADSCs were penetrated by the AuNPs and stored in vacuoles. The presence of the AuNPs in ADSCs resulted in increased population doubling times, decreased cell motility and cell-mediated collagen contraction. The degree to which the cells were impacted was a function of particle concentration, where the smaller particles required a sevenfold higher concentration to have the same effect as the larger ones. Furthermore, AuNPs reduced adipogenesis as measured by lipid droplet accumulation and adiponectin secretion. These effects correlated with transient increases in DLK1 and with relative reductions in fibronectin. Upon removal of exogenous AuNPs, cellular NP levels decreased and normal ADSC functions were restored. As adiponectin helps regulate energy metabolism, local fluctuations triggered by AuNPs can lead to systemic changes. Hence, careful choice of size, concentration and clinical application duration of AuNPs is warranted.
authors: Department of Medicine, Stem Cell Facility, State University of New York at Stony Brook , Stony Brook, NY , USA.
authors: Tatsiana
authors: T
authors: Mironava
authors: Michael
authors: M
authors: Hadjiargyrou
authors: Marcia
authors: M
authors: Simon
authors: Miriam H
authors: MH
authors: Rafailovich
mesh_terms: D050156:Adipogenesis / Q000187:drug effects; D052242:Adiponectin / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology; D000704:Analysis of Variance; D002135:Calcium-Binding Proteins; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000187:drug effects; D006046:Gold / Q000737:chemistry / Q000493:pharmacokinetics / Q000633:toxicity*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D042541:Intracellular Space / Q000737:chemistry / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects; D008565:Membrane Proteins / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D010316:Particle Size; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects*
chemical_list: D052242:Adiponectin; D002135:Calcium-Binding Proteins; C512941:DLK1 protein, human; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D006046:Gold