title: Native mass spectrometry of photosynthetic pigment-protein complexes.
abstract: Native mass spectrometry (MS), or as is sometimes called "native electrospray ionization" allows proteins in their native or near-native states in solution to be introduced into the gas phase and interrogated by mass spectrometry. This approach is now a powerful tool to investigate protein complexes. This article reviews the background of native MS of protein complexes and describes its strengths, taking photosynthetic pigment-protein complexes as examples. Native MS can be utilized in combination with other MS-based approaches to obtain complementary information to that provided by tools such as X-ray crystallography and NMR spectroscopy to understand the structure-function relationships of protein complexes. When additional information beyond that provided by native MS is required, other MS-based strategies can be successfully applied to augment the results of native MS.
authors: Department of Chemistry, Washington University in St. Louis, St. Louis, MO 63130, USA.
authors: Hao
authors: H
authors: Zhang
authors: Weidong
authors: W
authors: Cui
authors: Michael L
authors: ML
authors: Gross
authors: Robert E
authors: RE
authors: Blankenship
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001429:Bacteriochlorophylls / Q000737:chemistry / Q000378:metabolism; D002734:Chlorophyll / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D015394:Molecular Structure; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D045322:Photosynthetic Reaction Center Complex Proteins; D002734:Chlorophyll