title: The effect of cilostazol on the expression of matrix metalloproteinase-1 and type I procollagen in ultraviolet-irradiated human dermal fibroblasts.
abstract: OBJECTIVE
  Cilostazol is a selective inhibitor of type III phosphodiesterase that inhibits platelet aggregation. Cilostazol is a useful vasodilator, antithrombotic, and cardiotonic agent. Ultraviolet B (UVB) irradiation increases the production of matrix metalloproteinase-1 (MMP-1) during skin photoaging. The UVB-induced increase of MMP-1 results in connective tissue damage, and the skin becomes wrinkled and aged. Here, we investigated the capacity of cilostazol to inhibit MMP-1 expression in UVB-irradiated human dermal fibroblasts.
  
  
  METHODS
  Cultured human dermal fibroblasts were irradiated with UVB, followed by the addition of cilostazol to the culture medium.
  
  
  RESULTS
  Post-treatment with cilostazol attenuated UVB-induced production of MMP-1 and prevented the reduction of type I procollagen. Cilostazol inhibited UVB irradiation-induced phosphorylation of the mitogen-activated protein kinase (MAPK) signaling molecules Jun-N-terminal kinase (JNK) and p38 kinase, as well as activator protein-1 (AP-1) in dermal fibroblasts.
  
  
  CONCLUSIONS
  Overall, these results demonstrate that cilostazol regulates UVB-induced MMP-1 expression and type I procollagen synthesis by inhibiting MAPK signaling and AP-1 activity. Therefore, we suggest that cilostazol may be useful for the prevention and treatment of skin photodamage caused by UVB-irradiation.
authors: Department of Preventive Medicine, College of Medicine, Kosin University, Busan, Republic of Korea.
authors: Byeng Chul
authors: BC
authors: Yu
authors: Dae-Sung
authors: DS
authors: Lee
authors: Sang Mo
authors: SM
authors: Bae
authors: Won-Kyo
authors: WK
authors: Jung
authors: Jin Ho
authors: JH
authors: Chun
authors: Sang Hwa
authors: SH
authors: Urm
authors: Da-Young
authors: DY
authors: Lee
authors: Soo-Jin
authors: SJ
authors: Heo
authors: Sae-Gwang
authors: SG
authors: Park
authors: Su-Kil
authors: SK
authors: Seo
authors: Jae Wook
authors: JW
authors: Yang
authors: Jung Sik
authors: JS
authors: Choi
authors: Won Sun
authors: WS
authors: Park
authors: Il-Whan
authors: IW
authors: Choi
mesh_terms: D015153:Blotting, Western; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D000077407:Cilostazol; D024042:Collagen Type I / Q000096:biosynthesis*; D024202:Electrophoretic Mobility Shift Assay; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis*; D015394:Molecular Structure; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D015595:Skin Aging / Q000187:drug effects / Q000528:radiation effects; D013473:Sunscreening Agents / Q000494:pharmacology; D013777:Tetrazoles / Q000494:pharmacology*; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D024042:Collagen Type I; D017382:Reactive Oxygen Species; D013473:Sunscreening Agents; D013777:Tetrazoles; D018808:Transcription Factor AP-1; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1; D000077407:Cilostazol