title: Dual function of MIPS1 as a metabolic enzyme and transcriptional regulator.
abstract: Because regulation of its activity is instrumental either to support cell proliferation and growth or to promote cell death, the universal myo-inositol phosphate synthase (MIPS), responsible for myo-inositol biosynthesis, is a critical enzyme of primary metabolism. Surprisingly, we found this enzyme to be imported in the nucleus and to interact with the histone methyltransferases ATXR5 and ATXR6, raising the question of whether MIPS1 has a function in transcriptional regulation. Here, we demonstrate that MIPS1 binds directly to its promoter to stimulate its own expression by locally inhibiting the spreading of ATXR5/6-dependent heterochromatin marks coming from a transposable element. Furthermore, on activation of pathogen response, MIPS1 expression is reduced epigenetically, providing evidence for a complex regulatory mechanism acting at the transcriptional level. Thus, in plants, MIPS1 appears to have evolved as a protein that connects cellular metabolism, pathogen response and chromatin remodeling.
authors: Institut de Biologie des Plantes, UMR8618 Université Paris-Sud XI, 91405 Orsay, France.
authors: David
authors: D
authors: Latrasse
authors: Teddy
authors: T
authors: Jégu
authors: Pin-Hong
authors: PH
authors: Meng
authors: Christelle
authors: C
authors: Mazubert
authors: Elodie
authors: E
authors: Hudik
authors: Marianne
authors: M
authors: Delarue
authors: Céline
authors: C
authors: Charon
authors: Martin
authors: M
authors: Crespi
authors: Heribert
authors: H
authors: Hirt
authors: Cécile
authors: C
authors: Raynaud
authors: Catherine
authors: C
authors: Bergounioux
authors: Moussa
authors: M
authors: Benhamed
mesh_terms: D017209:Apoptosis; D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000378:metabolism / Q000502:physiology; D002467:Cell Nucleus / Q000201:enzymology; D042002:Chromatin Assembly and Disassembly; D003593:Cytoplasm / Q000201:enzymology; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005408:Flagellin / Q000276:immunology; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D006657:Histones / Q000378:metabolism; D018519:Meristem / Q000166:cytology / Q000201:enzymology / Q000235:genetics*; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism / Q000502:physiology; D007296:Myo-Inositol-1-Phosphate Synthase / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D057865:Plant Immunity / Q000235:genetics; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D021381:Protein Transport; D014026:Nicotiana
chemical_list: D029681:Arabidopsis Proteins; D006657:Histones; D005408:Flagellin; C542364:ATXR5 protein, Arabidopsis; C542365:ATXR6 protein, Arabidopsis; D008780:Methyltransferases; D007296:Myo-Inositol-1-Phosphate Synthase