title: Canine visceral leishmaniasis in boyer ahmad district, kohgiluyeh & boyer ahmad province, southwest of iran.
abstract: BACKGROUND
  Mediterranean type of visceral leishmaniasis (VL) is present in different parts of Iran. Several studies have identified dogs as the main reservoirs of the VL caused by Leishmania infantum in Iran and other Mediterranean regions. This study aimed to determine the seroprevalence of canine visceral leishmaniasis as animal reservoir host for human visceral leishmaniasis in Boyer Ahmad district in southwest of Iran.
  
  
  METHODS
  A seroepidemiological study was carried out to determine the seroprevalence of canine visceral leishmaniasis (CVL) among ownership dogs by using direct agglutination test (DAT) in 23 of 182 villages of Boyer Ahmad district, during August 2009 to August 2010. One hundred and seventy serum samples from ownership dogs were selected by multi-stage cluster sampling in villages of Boyer Ahmad district. All samples were tested by DAT and anti-Leishmania antibodies titers at ≥ 1:320 was considered as positive.
  
  
  RESULTS
  Of the 170 serum samples, 10% were positive by DAT at titers of 1:320 and higher. No statistical significant difference was found between male (10.7%) and female (8.3%) seroprevalence. The highest seroprevalence rate (15.1%) was observed among the ownership dogs of four to seven years age. Altogether, seventeen (25.4%) of the seropositive dogs had clinical signs and symptoms.
  
  
  CONCLUSIONS
  It seems that Boyer Ahmad district is an endemic area for canine visceral leishmaniasis in Iran.
authors: Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
authors: A
authors: A
authors: Moshfe
authors: M
authors: M
authors: Mohebali
authors: E
authors: E
authors: Afshoun
authors: A
authors: A
authors: Mousavizadeh
authors: Z
authors: Z
authors: Zarei
authors: H
authors: H
authors: Abidi
authors: B
authors: B
authors: Akhoundi
authors: V
authors: V
authors: Barati
authors: S
authors: S
authors: Joukar
keywords: Direct agglutination test; Iran; Ownership dogs; Visceral leishmaniasis