title: Preparation of dendritic cells by in vitro cultures.
abstract: In vitro cultures of bone marrow-derived precursors are a convenient method for generating dendritic cells (DC). This method additionally overcomes the problem of low availability of certain DC types, DC heterogeneity, and laborious procedures encountered using ex vivo isolation protocols.Here we describe two standard protocols for in vitro differentiation of steady-state DC equivalents with Fms-like tyrosine kinase 3 ligand (Flt3L) and inflammatory-like DC using granulocyte-macrophages-colony-stimulating factor (GM-CSF). These protocols allow for obtaining up to 2 × 10(8) CD11c(high) inflammatory-like DC and up to 5 × 10(6) equivalents of each CD8+ and CD8- conventional DC and plasmacytoid DC.
authors: Faculté de médecine René Descartes, INSERM U1013 and Université Paris Descartes, Sorbonne Paris Cité, Paris, France. mirjana.weimershaus@inserm.fr.
authors: Mirjana
authors: M
authors: Weimershaus
authors: Faculté de médecine René Descartes, INSERM U1013 and Université Paris Descartes, Sorbonne Paris Cité, Paris, France. peter.van-endert@inserm.fr.
authors: Peter
authors: P
authors: van Endert
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008024:Ligands; D051379:Mice; D051941:fms-Like Tyrosine Kinase 3 / Q000378:metabolism
chemical_list: D008024:Ligands; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D051941:fms-Like Tyrosine Kinase 3