title: Interaction of antitumor flavonoids with dsDNA in the absence and presence of Cu(II).
abstract: The binding of antitumor flavonoids, namely 3-hydroxyflavone (3HF) and hesperidin (Hesp) with dsDNA was investigated in the absence and presence of Cu(II) using cyclic voltammetry and square wave voltammetry at the hanging mercury drop electrode. The reduction currents of 3HF, 3HF-Cu complex, and the 3HF-β-cyclodextrin inclusion complex decreased after intercalation into dsDNA. The intercalation of Hesp into dsDNA is weak. dsDNA is reduced at a potential of -1.48 V overlaying the reduction of Hesp. In contrast, in the presence of Cu(II), the interaction of Hesp with dsDNA leads to a much stronger intercalation. The binding constants of the flavonoid-Cu complex with dsDNA were evaluated and calibration graphs for the determination of dsDNA were obtained from the decrease in the peak current in the cyclic voltammograms of 3HF in the presence of dsDNA. The proposed method exhibited good recovery and reproducibility for indirect determination of dsDNA.
authors: Chemistry Department, Faculty of Science, Assiut University, Assiut 71516, Egypt. Temerk44@yahoo.com
authors: Yassin M
authors: YM
authors: Temerk
authors: Mohamed S
authors: MS
authors: Ibrahim
authors: Mohamed
authors: M
authors: Kotb
authors: Wolfgang
authors: W
authors: Schuhmann
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D003300:Copper / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D055664:Electrochemical Techniques; D005419:Flavonoids / Q000494:pharmacology*; D006569:Hesperidin / Q000494:pharmacology*; D006801:Humans; D007364:Intercalating Agents / Q000494:pharmacology*; D012474:Salmon; D013816:Thermodynamics; D047392:beta-Cyclodextrins / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids; D007364:Intercalating Agents; D047392:beta-Cyclodextrins; D003300:Copper; D004247:DNA; D006569:Hesperidin; C031215:betadex; C041477:3-hydroxyflavone