title: [Effects of ezrin silencing on pancreatic cancer cell line Panc-1].
abstract: OBJECTIVE
  To explore the effects of ezrin silencing on pancreatic cancer cell line Panc-1.
  
  
  METHODS
  Pancreatic cancer cell line Panc-1 was transfected with ezrin silencing plasmid. The proliferation and the cell cycle status were determined by CCK-8 assay and flow cytometry analysis, respectively. Cellular membrane protrusions/microvilli formation were visualized by scanning election microscopy. Colony formation assay was used to determine the cell anchor-independent growth ability in vitro. Trans-filter migration and invasion assays were performed with 8 µm pore inserts in a 24-well BioCoat chamber with/without Matrigel.
  
  
  RESULTS
  Ezrin silencing decreased cellular protrusions/microvilli formation, anchorage-independent growth, cell migration and invasion, but had no effects on cell proliferation in vitro and cell cycle, in pancreatic cancer cell line Panc-1.
  
  
  CONCLUSIONS
  Ezrin expression affects the cellular protrusions/microvilli formation, anchorage-independent growth, cell migration and invasion in pancreatic cancer cell line Panc-1.
authors: Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100730, China.
authors: Yun-xiao
authors: YX
authors: Meng
authors: Shuang-ni
authors: SN
authors: Yu
authors: Zhao-hui
authors: ZH
authors: Lu
authors: Jie
authors: J
authors: Chen
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D022081:Cell Surface Extensions / Q000473:pathology; D003598:Cytoskeletal Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008871:Microvilli / Q000473:pathology; D009361:Neoplasm Invasiveness; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D010957:Plasmids; D034622:RNA Interference; D034741:RNA, Small Interfering; D014162:Transfection; D000099149:Ezrin
chemical_list: D003598:Cytoskeletal Proteins; D034741:RNA, Small Interfering; D000099149:Ezrin