title: Expression of the growth factor pleiotrophin and its receptor protein tyrosine phosphatase beta/zeta in the serum, cartilage and subchondral bone of patients with osteoarthritis.
abstract: OBJECTIVE
  Pleiotrophin is a heparin-binding growth factor expressed in embryonic but not mature cartilage, suggesting a role in cartilage development. Elucidation of the molecular changes observed during the remodelling process in osteoarthritis is of paramount importance. This study aimed to investigate serum pleiotrophin levels and expression of pleiotrophin and its receptor protein tyrosine phosphatase beta/zeta in the cartilage and subchondral bone of osteoarthritis patients.
  
  
  METHODS
  Serum samples derived from 16 osteoarthritis patients and 18 healthy donors. Pleiotrophin and receptor protein tyrosine phosphatase beta/zeta in the cartilage and subchondral bone were studied in 29 patients who had undergone total knee or hip replacement for primary osteoarthritis and in 10 control patients without macroscopic osteoarthritis changes.
  
  
  RESULTS
  Serum pleiotrophin levels and expression of pleiotrophin in chondrocytes and subchondral bone osteocytes significantly increased in osteoarthritis patients graded Ahlback II to III. Receptor protein tyrosine phosphatase beta/zeta was mainly detected in the subchondral bone osteocytes of patients with moderate osteoarthritis and as disease severity increased, in the osteocytes and bone lining cells of the distant trabeculae.
  
  
  CONCLUSIONS
  These data render pleiotrophin and receptor protein tyrosine phosphatase beta/zeta promising candidates for further studies towards developing targeted therapeutic schemes for osteoarthritis.
authors: Department of Pharmacy, Laboratory of Molecular Pharmacology, University of Patras, Patras GR 26504, Greece.
authors: Angelos
authors: A
authors: Kaspiris
authors: Constantinos
authors: C
authors: Mikelis
authors: Melanie
authors: M
authors: Heroult
authors: Lubna
authors: L
authors: Khaldi
authors: Theodoros B
authors: TB
authors: Grivas
authors: Ioannis
authors: I
authors: Kouvaras
authors: Spyridon
authors: S
authors: Dangas
authors: Elias
authors: E
authors: Vasiliadis
authors: Frédéric
authors: F
authors: Lioté
authors: José
authors: J
authors: Courty
authors: Evangelia
authors: E
authors: Papadimitriou
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019644:Arthroplasty, Replacement, Hip; D019645:Arthroplasty, Replacement, Knee; D015415:Biomarkers / Q000378:metabolism; D001842:Bone and Bones / Q000378:metabolism* / Q000473:pathology; D002352:Carrier Proteins / Q000378:metabolism*; D002356:Cartilage / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D019902:Chondrocytes / Q000378:metabolism / Q000473:pathology; D016207:Cytokines / Q000378:metabolism*; D004185:Disability Evaluation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015207:Osteoarthritis, Hip / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D020370:Osteoarthritis, Knee / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D010011:Osteocytes / Q000378:metabolism / Q000473:pathology; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5 / Q000378:metabolism*; D012720:Severity of Illness Index
chemical_list: D015415:Biomarkers; D002352:Carrier Proteins; D016207:Cytokines; C066845:pleiotrophin; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5
keywords: Bone-lining cells; Chondrocytes; Osteoarthritis; Osteocytes; Pleiotrophin; Protein tyrosine phosphatase