title: Skin lipid profiling in normal and seborrhoeic shih tzu dogs.
abstract: BACKGROUND
  Seborrhoea is a clinical condition resulting in excessive lipid and/or scale on the skin and is a common and important skin disease of dogs. However, there is little information on the skin surface lipid composition of dogs with seborrhoea.
  
  
  OBJECTIVE
  To compare skin surface lipid profiles in normal and seborrhoeic shih tzu dogs.
  
  
  METHODS
  Fourteen client-owned dogs (seven seborrhoeic and seven normal) were investigated. Lipids in sebaceous glands (SGs) were extracted from homogenized tissues of SG hyperplasia. Surface lipid was collected by tape stripping [stratum corneum (SC)-enriched fraction] and acetone-wetted cotton swab (acetone-extracted fraction). Lipids in SGs, SC-enriched fractions and acetone-extracted fractions were evaluated by high-performance thin-layer chromatography.
  
  
  RESULTS
  Lipids in SGs mainly consisted of cholesterol esters, wax esters and triglycerides, whereas lipids in the SC-enriched fraction mainly consisted of ceramides. The acetone-extracted fraction contained a mixture of lipid classes recognized in SG- and SC-enriched fractions. In seborrhoeic dogs, concentrations of wax esters and triglycerides in the acetone-extracted fraction were significantly higher than in control dogs (P = 0.0285). Amounts of total ceramides (in micrograms) per milligram of SC were not significantly different between the two groups (P = 0.5204). Interestingly, two unknown ceramide fractions, which accounted for 20% of the total ceramides, were recognized exclusively in seborrhoeic dogs.
  
  
  CONCLUSIONS
  These results provide evidence that the skin surface lipid profiles are altered in shih tzu dogs with seborrhoea.
authors: Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan.
authors: Ji-Seon
authors: JS
authors: Yoon
authors: Koji
authors: K
authors: Nishifuji
authors: Sinpei
authors: S
authors: Ishioroshi
authors: Kaori
authors: K
authors: Ide
authors: Toshiroh
authors: T
authors: Iwasaki
mesh_terms: D000818:Animals; D016022:Case-Control Studies; D012628:Dermatitis, Seborrheic / Q000378:metabolism / Q000662:veterinary*; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D004817:Epidermis / Q000378:metabolism; D005260:Female; D050356:Lipid Metabolism / Q000502:physiology*; D008297:Male; D012867:Skin / Q000378:metabolism*