title: A preliminary study of the antibacterial potential of cetylpyridinium chloride in root canals infected by E. faecalis.
abstract: The aim of this preliminary study was to verify the antibacterial potential of cetylpyridinium chloride (CPC) in root canals infected by Enterococcus faecalis. Forty human maxillary anterior teeth were prepared and inoculated with E. faecalis for 60 days. The teeth were randomly assigned to the following groups: 1: Root canal preparation (RCP) + 0.1% CPC with positive-pressure irrigation (PPI, Conventional, NaviTip(®)); 2: RCP + 0.2% CPC PPI; 3: RCP + 2.5% NaOCl PPI; 4: RCP + 2.5% NaOCl with negative-pressure irrigation system (NPI, EndoVac(®)); 5: Positive control; and 6: Negative control. Four teeth of each experimental group were evaluated by culture and 4 by scanning electron microscopy (SEM). In all teeth, the root canals were dried and filled with 17% EDTA (pH 7.2) for 3 min for smear layer removal. Samples from the infected root canals were collected and immersed in 7 mL of Letheen Broth (LB), followed by incubation at 37°C for 48 h. Bacterial growth was analyzed by turbidity of culture medium and then observed with a UV spectrophotometer. The irrigating solutions were further evaluated for antimicrobial effect by an agar diffusion test.The statistical data were treated by means, standard deviation, Kruskal-Wallis test and analysis of variance. Significance level was set at 5%. The results showed the presence of E. faecalis after root canal sanitization. The number of bacteria decreased after the use of CPC. In the agar diffusion test, CPC induced large microbial inhibition zones, similar to 2% chlorhexidine and large than 2.5% NaOCl. In conclusion, cetylpyridinium chloride showed antibacterial potential in endodontic infection with E. faecalis.
authors: Dental School, UFG - Federal University of Goiás, Goiânia, GO, Brazil. estrela3@terra.com.br
authors: Carlos
authors: C
authors: Estrela
authors: Manoel Damião
authors: MD
authors: Sousa-Neto
authors: Denise Ramos Silveira
authors: DR
authors: Alves
authors: Ana Helena Gonçalves
authors: AH
authors: Alencar
authors: Tatiane Oliveira
authors: TO
authors: Santos
authors: Jesus Djalma
authors: JD
authors: Pécora
mesh_terms: D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D058491:Bacterial Load / Q000187:drug effects; D001431:Bacteriological Techniques; D002594:Cetylpyridinium / Q000494:pharmacology*; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004492:Edetic Acid / Q000494:pharmacology; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D005779:Immunodiffusion; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D009391:Nephelometry and Turbidimetry; D011312:Pressure; D012388:Root Canal Irrigants / Q000494:pharmacology*; D018915:Root Canal Preparation / Q000379:methods; D016369:Smear Layer; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D007507:Therapeutic Irrigation / Q000379:methods; D013997:Time Factors
chemical_list: D000891:Anti-Infective Agents, Local; D012388:Root Canal Irrigants; D004492:Edetic Acid; D002594:Cetylpyridinium; D012973:Sodium Hypochlorite