title: Recombinant truncated AniA of pathogenic Neisseria elicits a non-native immune response and functional blocking antibodies.
abstract: AniA of the pathogenic Neisseria is glycosylated in its C-terminal repeat region by the pilin glycosylation (pgl) pathway. AniA appears to be unique among bacterial nitrite reductases as it contains an N-terminal extension that includes a lipid modification site as well as a C-terminal extension that is glycosylated. Immunising with various glycoforms of the AniA protein demonstrated a strong humoral immune response to the basal monosaccharide. In addition, when animals were immunised with a truncated form of AniA, completely lacking the glycosylated C-terminal region, the antibody response was directed against AniA regardless of the glycosylation state of the protein. Immuno-SEM confirmed that AniA is expressed on the cell surface in Neisseria gonorrhoeae. Antisera generated against a truncated, non-glycosylated, recombinant form of the AniA protein are capable of blocking nitrite reductase function in a whole cell assay. We propose that recombinant modified AniA has potential as a vaccine antigen for N. gonorrhoeae.
authors: Institute for Glycomics, Griffith University, Gold Coast, Queensland 4222, Australia.
authors: Lucy K
authors: LK
authors: Shewell
authors: Shan C
authors: SC
authors: Ku
authors: Benjamin L
authors: BL
authors: Schulz
authors: Freda E-C
authors: FE
authors: Jen
authors: Tsitsi D
authors: TD
authors: Mubaiwa
authors: Margaret R
authors: MR
authors: Ketterer
authors: Michael A
authors: MA
authors: Apicella
authors: Michael P
authors: MP
authors: Jennings
mesh_terms: D019138:Antibodies, Blocking / Q000096:biosynthesis* / Q000276:immunology; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology; D006031:Glycosylation; D009344:Neisseria gonorrhoeae / Q000201:enzymology / Q000276:immunology*; D009572:Nitrite Reductases / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism
chemical_list: D019138:Antibodies, Blocking; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D011994:Recombinant Proteins; C077531:aniA protein, Neisseria gonorrhoeae; D009572:Nitrite Reductases