title: Leishmania major, the predominant Leishmania species responsible for cutaneous leishmaniasis in Mali.
abstract: Leishmania major is the only species of Leishmania known to cause cutaneous leishmanisis (CL) in Mali. We amplified Leishmania DNA stored on archived Giemsa-stained dermal scraping slides obtained from self-referral patients with clinically suspected CL seen in the Center National d'Appui A La Lutte Contre La Maladie (CNAM) in Bamako, Mali, to determine if any other Leishmania species were responsible for CL in Mali and evaluate its geographic distribution. Polymerase chain reaction (PCR) amplification was performed using a Leishmania species-specific primer pair that can amplify DNA from L. major, L. tropica, L. infantum, and L. donovani parasites, possible causative agents of CL in Mali. L. major was the only species detected in 41 microscopically confirmed cases of CL from five regions of Mali (Kayes, Koulikoro, Ségou, Mopti, and Tombouctou). These results implicate L. major as the predominant, possibly exclusive species responsible for CL in Mali.
authors: Section of Dermatology, University of Chicago, Chicago, IL, USA. Carlos.Paz@uchospitals.edu
authors: Carlos
authors: C
authors: Paz
authors: Sibiry
authors: S
authors: Samake
authors: Jennifer M
authors: JM
authors: Anderson
authors: Ousmane
authors: O
authors: Faye
authors: Pierre
authors: P
authors: Traore
authors: Koureishi
authors: K
authors: Tall
authors: Moumine
authors: M
authors: Cisse
authors: Somita
authors: S
authors: Keita
authors: Jesus G
authors: JG
authors: Valenzuela
authors: Seydou
authors: S
authors: Doumbia
mesh_terms: D018105:DNA, Kinetoplast / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D006801:Humans; D018320:Leishmania major / Q000145:classification / Q000235:genetics*; D016773:Leishmaniasis, Cutaneous / Q000453:epidemiology* / Q000469:parasitology*; D008302:Mali / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D011159:Population Surveillance; D012680:Sensitivity and Specificity; D012867:Skin / Q000469:parasitology; D013045:Species Specificity
chemical_list: D018105:DNA, Kinetoplast; D016054:DNA, Protozoan