title: Occurrence of stilbene oligomers in Cyperus rhizomes.
abstract: Investigation of the chemical constituents of Rhizoma Cyperi (Cyperus rotundus Linneus)resulted in the isolation of novel enantiomeric andmeso-stilbene trimers [i.e., (+)- and (−)-(E)-cyperusphenol A (1, 2 respectively) and (E)-mesocyperusphenol A (3)], a trimer bearing a novelhexacyclic ring system [cyperusphenol B (5)], aswell as knownstilbenoids (cyperusphenols C (4)and D (6), scirpusins A (7) and B (8), and piceid (9)) and luteolin. HPLC was used for the opticalresolution of 1 and 2 as well as for the identification of cooccurrence of enantiomers of 7. Thestructures of the isolates were established by spectroscopic analyses, including a detailed NMRspectroscopic investigation. The isolates were evaluated in terms of their antiproliferative activityemploying the Jurkat cell line (human T-cell leukemia cells), while the IC50 potencies of aracemate of 1 and 2, 3, 5, and 6 were estimated as 27.4, 40.5, 26.4, and 26.3 μM, respectively. Thesuppression of cell growth by 6 was due to the induction of apoptosis,whichwas characterized bynuclear changes and PARP-1 cleavage determined bywestern blotting.We also evaluated the freeradical scavenging activity of the isolates.
authors: Laboratory of Pharmacognosy, Gifu Pharmaceutical University, 1-25-4 Daigaku-nishi, Gifu 501-1196, Japan. teito@gifu-pu.ac.jp
authors: Tetsuro
authors: T
authors: Ito
authors: Hidetatsu
authors: H
authors: Endo
authors: Haruka
authors: H
authors: Shinohara
authors: Masayoshi
authors: M
authors: Oyama
authors: Yukihiro
authors: Y
authors: Akao
authors: Munekazu
authors: M
authors: Iinuma
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology; D029786:Cyperus / Q000737:chemistry*; D006801:Humans; D019169:Jurkat Cells; D015394:Molecular Structure; D027343:Rhizome / Q000737:chemistry*; D013267:Stilbenes / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D013267:Stilbenes