title: Unraveling supported lipid bilayer formation kinetics: osmotic effects.
abstract: Solid-supported lipid bilayers are used as cell membrane models and form the basis of biomimetic and biosensor platforms. The mechanism of their formation from adsorbed liposomes is not well-understood. Using membrane-permeable solute glycerol, impermeable solutes sucrose and dextran, and a pore forming peptide melittin, we studied experimentally how osmotic effects affect the kinetics of the adsorbed liposome-to-bilayer transition. We find that its rate is enhanced if adsorbed liposomes are made permeable but is not significantly retarded by impermeable solutes. The results are explained in terms of adsorbed liposome deformation and formation of transmembrane pores.
authors: CIC biomaGUNE, Paseo Miramón 182, San Sebastián 20009, Spain.
authors: Nicole
authors: N
authors: Hain
authors: Marta
authors: M
authors: Gallego
authors: Ilya
authors: I
authors: Reviakine
mesh_terms: D003911:Dextrans / Q000737:chemistry; D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry*; D008555:Melitten / Q000737:chemistry; D009995:Osmosis; D013395:Sucrose / Q000737:chemistry
chemical_list: D003911:Dextrans; D008051:Lipid Bilayers; D008555:Melitten; D013395:Sucrose