title: Production of CD4⁺ and CD8⁺ T cell hybridomas.
abstract: T cell hybridomas are very useful tools to investigate antigen presenting cell (APC) function. They were developed based on the fusion technology that led to monoclonal antibody section. Antigen-specific primary T cells are generated and fused to an immortal thymoma line. Unfused thymoma cells are eliminated by engineered metabolic selection. Antigen-specific hybridomas are identified and may be characterized in detail. Primary T cells are preferable for studies of the regulatory mechanisms intrinsic to T cells, but for study of antigen presentation T cell hybridomas have advantages over primary T cell clones, including their relative uniformity, stability over time, and ready availability in large numbers for extensive antigen presentation experiments.
authors: Division of Infectious Disease, Case Western Reserve University, Cleveland, OH, USA. dxc44@case.edu.
authors: David H
authors: DH
authors: Canaday
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002459:Cell Fusion / Q000379:methods*; D002469:Cell Separation; D002999:Clone Cells; D015925:Cryopreservation; D006825:Hybridomas / Q000166:cytology* / Q000276:immunology; D007114:Immunization; D051379:Mice
chemical_list: D000941:Antigens