title: Vaccine protection against Bacillus cereus-mediated respiratory anthrax-like disease in mice.
abstract: Bacillus cereus strains harboring a pXO1-like virulence plasmid cause respiratory anthrax-like disease in humans, particularly in welders. We developed mouse models for intraperitoneal as well as aerosol challenge with spores of B. cereus G9241, harboring pBCXO1 and pBC218 virulence plasmids. Compared to wild-type B. cereus G9241, spores with a deletion of the pBCXO1-carried protective antigen gene (pagA1) were severely attenuated, whereas spores with a deletion of the pBC218-carried protective antigen homologue (pagA2) were not. Anthrax vaccine adsorbed (AVA) immunization raised antibodies that bound and neutralized the pagA1-encoded protective antigen (PA1) but not the PA2 orthologue encoded by pagA2. AVA immunization protected mice against a lethal challenge with spores from B. cereus G9241 or B. cereus Elc4, a strain that had been isolated from a fatal case of anthrax-like disease. As the pathogenesis of B. cereus anthrax-like disease in mice is dependent on pagA1 and PA-neutralizing antibodies provide protection, AVA immunization may also protect humans from respiratory anthrax-like death.
authors: Howard Taylor Ricketts Laboratory, Argonne National Laboratory, Argonne, Illinois, USA.
authors: So-Young
authors: SY
authors: Oh
authors: Hannah
authors: H
authors: Maier
authors: Jay
authors: J
authors: Schroeder
authors: G Stefan
authors: GS
authors: Richter
authors: Derek
authors: D
authors: Elli
authors: James M
authors: JM
authors: Musser
authors: Lauriane E
authors: LE
authors: Quenee
authors: Dominique M
authors: DM
authors: Missiakas
authors: Olaf
authors: O
authors: Schneewind
mesh_terms: D000818:Animals; D001409:Bacillus cereus / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D005260:Female; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology / Q000517:prevention & control*; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000517:prevention & control*
chemical_list: D001428:Bacterial Vaccines