title: Development of a microwave-assisted-extraction-based method for the determination of aflatoxins B1, G1, B2, and G2 in grains and grain products.
abstract: This article describes the use of microwave-assisted extraction (MAE) as a pretreatment technique for the determination of aflatoxins B(1), G(1), B(2), and G(2) in grains and grain products. The optimal operation parameters, including extraction solvent, temperature, and time, were identified to be acetonitrile as the extraction solvent at 80 °C with 15 min of MAE. The extracts were cleaned up using solid-phase extraction followed by derivatization with trifluoroacetic acid and were determined by liquid chromatography-fluorescence detection. A Sep-Pak cartridge was chosen over Oasis HLB and Bond Elut cartridges. By the use of aflatoxin M(1) as an internal standard, relative recoveries of the aflatoxins ranged from 90.7 to 105.7 % for corn and from 88.1 to 103.4 % for wheat, with relative standard deviations between 2.5 and 8.7 %. A total of 36 samples from local markets were analyzed, and aflatoxin B(1) was found to be the predominant toxin, with concentrations ranging from 0.42 to 3.41 μg/kg.
authors: School of Food Science and Biological Engineering, Zhejiang Gongshang University, Hangzhou, People's Republic of China.
authors: Si
authors: S
authors: Chen
authors: Hong
authors: H
authors: Zhang
mesh_terms: D016604:Aflatoxin B1 / Q000302:isolation & purification*; D000348:Aflatoxins / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002523:Edible Grain / Q000737:chemistry*; D008872:Microwaves; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D014908:Triticum / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry
chemical_list: D000348:Aflatoxins; C027955:aflatoxin G1; C029754:aflatoxin G2; C029753:aflatoxin B2; D016604:Aflatoxin B1