title: Specific activity of electron-beam synthesis immobilized hyaluronidase on G-CSF induced mobilization of bone marrow progenitor cells.
abstract: The effects of nanotechnology (electron-beam) -PEGylated (or immobilized; Im) hyaluronidase (HD) on the state of the pool of bone marrow progenitor cells and their mobilization induced by granulocyte colony stimulating factor (G-CSF) were studied. A high specific activity of the drug Im-HD on progenitor cells of different classes was demonstrated using parenteral and enteral administration. An increase in the content of erythroid (E), granulomonocytic (GM), fibroblast (F) colony-forming units (CFU) and mesenchymal stem cells (MSC) in bone marrow was shown, as well as G-CSF-induced stimulation of mobilization of precursors into the peripheral blood under the influence of Im-HD. The detected activity of this novel drug on progenitor cells indicates the potential for a safe and highly effective treatment for hematology practice and regenerative medicine.
authors: Federal State Budgetary Institution, Research Institute of Pharmacology under the Siberian Branch of Russian Academy of Medical Sciences, Tomsk, Russia.
authors: A M
authors: AM
authors: Dygai
authors: G N
authors: GN
authors: Zyuz'kov
authors: V V
authors: VV
authors: Zhdanov
authors: E V
authors: EV
authors: Udut
authors: L A
authors: LA
authors: Miroshnichenko
authors: E V
authors: EV
authors: Simanina
authors: T Yu
authors: TY
authors: Khrichkova
authors: M Yu
authors: MY
authors: Minakova
authors: P G
authors: PG
authors: Madonov
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D003114:Colony-Forming Units Assay; D060733:Electromagnetic Radiation; D004583:Electrons; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006821:Hyaluronoglucosaminidase / Q000008:administration & dosage* / Q000737:chemistry; D055767:Immobilized Proteins / Q000008:administration & dosage* / Q000737:chemistry; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008808:Mice, Inbred CBA; D011092:Polyethylene Glycols / Q000737:chemistry
chemical_list: D055767:Immobilized Proteins; D016179:Granulocyte Colony-Stimulating Factor; D011092:Polyethylene Glycols; D006821:Hyaluronoglucosaminidase