title: ZFX regulates glioma cell proliferation and survival in vitro and in vivo.
abstract: The zinc finger transcription factor ZFX functions as an important regulator of self-renewal in multiple stem cell types, as well as a sex determinant of mammals. Moreover, ZFX expression is abnormally elevated in several cancers, and correlates with malignancy grade. To investigate its role in the pathogenesis of gliomas, we used lentivirus-mediated RNA interference (RNAi) to knockdown ZFX expression in human glioma cell lines. Our results demonstrate that ZFX plays a crucial role in glioma proliferation and survival, confirming recent reports. We also show for the first time that ZFX knockdown decreases the in vivo growth potential of U87 glioma xenografts in both subcutaneous and intracranial models in nude mice. We conclude that lentivirus-mediated RNAi targeting of ZFX may serve as a promising strategy for glioma therapy.
authors: School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People's Republic of China.
authors: Zhichuan
authors: Z
authors: Zhu
authors: Kui
authors: K
authors: Li
authors: Dafeng
authors: D
authors: Xu
authors: Yongjie
authors: Y
authors: Liu
authors: Hailiang
authors: H
authors: Tang
authors: Qing
authors: Q
authors: Xie
authors: Liqian
authors: L
authors: Xie
authors: Jiwei
authors: J
authors: Liu
authors: Hongtao
authors: H
authors: Wang
authors: Ye
authors: Y
authors: Gong
authors: Zelan
authors: Z
authors: Hu
authors: Jing
authors: J
authors: Zheng
mesh_terms: D000375:Aging / Q000187:drug effects; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D005434:Flow Cytometry; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D020287:In Situ Nick-End Labeling; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism*; D016086:Lentivirus / Q000235:genetics / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D008819:Mice, Nude; D051058:Oncogene Protein v-akt / Q000378:metabolism; D034741:RNA, Small Interfering / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors; D014410:Tumor Stem Cell Assay; D014415:Tunicamycin / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics; D023041:Xenograft Model Antitumor Assays; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D000998:Antiviral Agents; D051741:Kruppel-Like Transcription Factors; D034741:RNA, Small Interfering; C062076:zinc finger protein, X-linked; D014415:Tunicamycin; D051058:Oncogene Protein v-akt; D001616:beta-Galactosidase