title: One-stage tissue engineering of bladder wall patches for an easy-to-use approach at the surgical table.
abstract: We present a method for producing a cell-scaffold hybrid construct at the bedside. The construct is composed of plastic-compressed collagen together with a poly(ɛ-caprolactone) (PCL)-knitted mesh that yields an integrated, natural-synthetic scaffold. This construct was evaluated by seeding of minced bladder mucosa, followed by proliferation in vitro. High mechanical strength in combination with a biological environment suitable for tissue growth was achieved through the creation of a hybrid construct that showed an increased tensile strength (17.9 ± 2.6 MPa) when compared to plastic-compressed collagen (0.6 ± 0.12 MPa). Intimate contact between the collagen and the PCL fabric was required to ensure integrity without delamination of the construct. This contact was achieved by surface alkaline hydrolysis of the PCL, followed by adsorption of poly(vinyl) alcohol. The improvement in hydrophilicity of the PCL-knitted mesh was confirmed through water contact angle measurements, and penetration of the collagen into the mesh was evaluated by scanning electron microscopy (SEM). Particles of minced bladder mucosa tissue were seeded onto this scaffold, and the proliferation was followed for 6 weeks in vitro. Results obtained from phase contrast microscopy, SEM, and histological staining indicated that cells migrated from the minced tissue particles and reorganized on the scaffold. Cells were viable and proliferative, with morphological features characteristic of urothelial cells. Proliferation reached the point at which a multilayer with a resemblance to stratified urothelium was achieved. This successful method could potentially be used for in vivo applications in reconstructive urology as an engineered autologous tissue transplant without the requirement for in vitro culture before transplantation.
authors: Textile Department, Isfahan University of Technology, Isfahan, Iran.
authors: Fatemeh
authors: F
authors: Ajalloueian
authors: Said
authors: S
authors: Zeiai
authors: Ramiro
authors: R
authors: Rojas
authors: Magdalena
authors: M
authors: Fossum
authors: Jöns
authors: J
authors: Hilborn
mesh_terms: D000818:Animals; D003094:Collagen / Q000494:pharmacology; D019245:Compressive Strength / Q000187:drug effects; D008855:Microscopy, Electron, Scanning; D010969:Plastics / Q000494:pharmacology; D011091:Polyesters / Q000494:pharmacology; D051381:Rats; D013499:Surface Properties; D013526:Surgical Mesh; D013718:Tensile Strength / Q000187:drug effects; D013784:Textiles; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D001743:Urinary Bladder / Q000601:surgery*
chemical_list: D010969:Plastics; D011091:Polyesters; C016240:polycaprolactone; D003094:Collagen