title: Induction of the BCMO1 gene during the suckling-weaning transition in rats is associated with histone H3 K4 methylation and subsequent coactivator binding and histone H3 acetylation to the gene.
abstract: The cells involved in nutrient absorption in the small intestine of rats undergo rapid maturation during the suckling-weaning transition period, i.e., 2-4 wk after birth. During this period, the serum thyroid hormone level is increased. However, the molecular mechanisms involved in the regulation of β-carotene 15,15'-monooxygenase 1 (BCMO1) gene expression in the small intestine remain unknown. In this study, we found that jejunal β-carotene 15,15' dioxygenase activity and the gene expression of BCMO1 were significantly increased during this transition period between days 13 and 27 after birth. A chromatin immunoprecipitation assay revealed that di- and tri-methylation of histone H3 at lysine 4 (K4) and the binding of thyroid hormone receptor (TR) α-1 binding on the promoter/enhancer and/or transcribed regions of the BCMO1 gene were enhanced from the earlier stage of weaning (i.e., 20 d after birth), prior to an enhancement of the acetylation of histone H3 and the binding of coactivator (SRC-1 and CBP) to the promoter/enhancer and/or transcribed regions of the BCMO1 gene, which was apparent at 27 d after birth. These results suggest that histone H3 K4 methylation and TRα-1 binding on the BCMO1 gene during the suckling-weaning transient period in rats predisposes to subsequent coactivator recruitment and histone H3 acetylation on the gene.
authors: Laboratory of Nutritional Physiology, Graduate School of Nutritional and Environmental Sciences and Global COE Program, The University of Shizuoka, Shizuoka, Japan.
authors: Hiroko
authors: H
authors: Mochizuki
authors: Kazuki
authors: K
authors: Mochizuki
authors: Kazuhito
authors: K
authors: Suruga
authors: Miki
authors: M
authors: Igarashi
authors: Sachiko
authors: S
authors: Takase
authors: Toshinao
authors: T
authors: Goda
mesh_terms: D000107:Acetylation; D000818:Animals; D000833:Animals, Suckling; D047369:Chromatin Immunoprecipitation; D005786:Gene Expression Regulation; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism; D008239:Lysine / Q000378:metabolism*; D008745:Methylation; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D037021:Thyroid Hormone Receptors alpha / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014886:Weaning; D050559:beta-Carotene 15,15'-Monooxygenase / Q000235:genetics / Q000378:metabolism*
chemical_list: C493357:Bco1 protein, rat; D006657:Histones; D012333:RNA, Messenger; D037021:Thyroid Hormone Receptors alpha; D014157:Transcription Factors; D050559:beta-Carotene 15,15'-Monooxygenase; D008239:Lysine