title: Rapid characterization of hydrogen exchange in proteins.
abstract: Kinetics and thermodynamics of amide hydrogen exchange in proteins can be investigated with two-dimensional (13)CO-(15)N NMR correlation experiments. The spectra are acquired with high resolution and sensitivity. A single type of experiment on one sample serves to characterize hydrogen-deuterium fractionation factors and hydrogen-exchange rates that span three orders of magnitude.
authors: NMR Research Centre, Indian Institute of Science, Bangalore-560012, India.
authors: Anushikha
authors: A
authors: Thakur
authors: Kousik
authors: K
authors: Chandra
authors: Abhinav
authors: A
authors: Dubey
authors: Patrick
authors: P
authors: D'Silva
authors: Hanudatta S
authors: HS
authors: Atreya
mesh_terms: D000577:Amides / Q000737:chemistry / Q000378:metabolism; D006859:Hydrogen / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008958:Models, Molecular; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D013816:Thermodynamics; D025801:Ubiquitin / Q000737:chemistry / Q000378:metabolism
chemical_list: D000577:Amides; D011506:Proteins; D025801:Ubiquitin; D006859:Hydrogen