title: A genome-wide methylation study on essential hypertension in young African American males.
abstract: OBJECTIVE
  There is emerging evidence from animal studies suggesting a key role for methylation in the pathogenesis of essential hypertension. However, to date, very few studies have investigated the role of methylation in the development of human hypertension, and none has taken a genome-wide approach. Based on the recent studies that highlight the involvement of inflammation in the development of hypertension, we hypothesize that changes in DNA methylation of leukocytes are involved in the pathogenesis of hypertension.
  
  
  RESULTS
  We conducted a genome-wide methylation analysis on 8 hypertensive cases and 8 normotensive age-matched controls aged 14-23 years and performed validation of the most significant CpG sites in 2 genes in an independent sample of 36 hypertensive cases and 60 normotensive controls aged 14-30 years. Validation of the CpG sites in the SULF1 gene was further conducted in a second replication sample of 36 hypertensive cases and 34 controls aged 15.8-40 years. A CpG site in the SULF1 gene showed higher methylation levels in cases than in healthy controls in the genome-wide step (p = 6.2×10(-5)), which was confirmed in the validation step (p = 0.011) for subjects ≤30 years old but was not significant for subjects of all ages combined (p = 0.095).
  
  
  CONCLUSIONS
  The identification of a difference in a blood leukocyte DNA methylation site between hypertensive cases and normotensive controls suggests that changes in DNA methylation may play an important role in the pathogenesis of hypertension. The age dependency of the effect further suggests complexity of epigenetic regulation in this age-related disease.
authors: Georgia Prevention Institute, Department of Pediatrics, Georgia Health Sciences University, Augusta, Georgia, United States of America. xwang@georgiahealth.edu
authors: Xiaoling
authors: X
authors: Wang
authors: Bonita
authors: B
authors: Falkner
authors: Haidong
authors: H
authors: Zhu
authors: Huidong
authors: H
authors: Shi
authors: Shaoyong
authors: S
authors: Su
authors: Xiaojing
authors: X
authors: Xu
authors: Ashok Kumar
authors: AK
authors: Sharma
authors: Yanbin
authors: Y
authors: Dong
authors: Frank
authors: F
authors: Treiber
authors: Bernard
authors: B
authors: Gutin
authors: Gregory
authors: G
authors: Harshfield
authors: Harold
authors: H
authors: Snieder
mesh_terms: D000293:Adolescent; D001741:Black or African American / Q000235:genetics; D001794:Blood Pressure / Q000235:genetics; D018899:CpG Islands / Q000235:genetics*; D019175:DNA Methylation / Q000235:genetics*; D015894:Genome, Human; D006801:Humans; D006973:Hypertension / Q000097:blood / Q000235:genetics* / Q000503:physiopathology; D007249:Inflammation; D007962:Leukocytes / Q000166:cytology; D008297:Male; D015238:Sulfotransferases / Q000097:blood*; D055815:Young Adult
chemical_list: C470791:SULF1 protein, human; D015238:Sulfotransferases