title: Innate and adaptive immune responses to the major Parietaria allergen Par j 1 in healthy subjects.
abstract: In this study we wanted to analyse the pattern of the immune response to the Parietaria major allergen Par j 1 in freshly purified peripheral blood mononuclear cell (PBMC) from healthy subjects. We observed that Par j 1 was capable of inducing IFN-γ production by CD3⁻ and CD16⁺/CD56⁺ cells exclusively in healthy individuals. Furthermore, a multiparametric analysis allowed us a better definition of two IFN-γ-Par j 1 specific populations (IFN-γ(dim) and IFN-γ(high)) characterized by the presence of different proportions of NKT and NK cells. We also identified the concomitant presence of a subset of IL-10⁺ NK cells. Moreover, CFSE staining showed that the Par j 1 preferentially induced the proliferation of CD3⁻/CD56⁺/CD335⁺ cells. Finally, a subset of CD4⁺/CD25⁺/FoxP3⁺/IL-10⁻ T cells was identified. The result of this pilot study suggest that during a tolerogenic response, the major allergen of the Parietaria pollen works as an activator of both the innate and the adaptive human immune system.
authors: Istituto di Biomedicina ed Immunologia Molecolare "Alberto Monroy" del Consiglio Nazionale delle Ricerche, Palermo, Italy.
authors: A
authors: A
authors: Bonura
authors: S
authors: S
authors: Quaratino
authors: F
authors: F
authors: Gervasi
authors: M R
authors: MR
authors: Melis
authors: C
authors: C
authors: Di Sano
authors: P
authors: P
authors: Colombo
mesh_terms: D056704:Adaptive Immunity; D000328:Adult; D000485:Allergens / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000276:immunology; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007113:Immunity, Innate; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology; D007694:Killer Cells, Natural / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D055611:Natural Killer T-Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D032403:Parietaria / Q000276:immunology*; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology
chemical_list: D000485:Allergens; D015703:Antigens, CD; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; C508609:IL10 protein, human; C083860:Par j I protein, Parietaria judaica; D010940:Plant Proteins; D011994:Recombinant Proteins; D016753:Interleukin-10; D007371:Interferon-gamma