title: Dynamics and biological relevance of DNA demethylation in Arabidopsis antibacterial defense.
abstract: DNA methylation is an epigenetic mark that silences transposable elements (TEs) and repeats. Whereas the establishment and maintenance of DNA methylation are relatively well understood, little is known about their dynamics and biological relevance in plant and animal innate immunity. Here, we show that some TEs are demethylated and transcriptionally reactivated during antibacterial defense in Arabidopsis. This effect is correlated with the down-regulation of key transcriptional gene silencing factors and is partly dependent on an active demethylation process. DNA demethylation restricts multiplication and vascular propagation of the bacterial pathogen Pseudomonas syringae in leaves and, accordingly, some immune-response genes, containing repeats in their promoter regions, are negatively regulated by DNA methylation. This study provides evidence that DNA demethylation is part of a plant-induced immune response, potentially acting to prime transcriptional activation of some defense genes linked to TEs/repeats.
authors: Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8197, Institut National de la Santé et de la Recherche Médicale, Unité 1024, 75005 Paris, France.
authors: Agnès
authors: A
authors: Yu
authors: Gersende
authors: G
authors: Lepère
authors: Florence
authors: F
authors: Jay
authors: Jingyu
authors: J
authors: Wang
authors: Laure
authors: L
authors: Bapaume
authors: Yu
authors: Y
authors: Wang
authors: Anne-Laure
authors: AL
authors: Abraham
authors: Jon
authors: J
authors: Penterman
authors: Robert L
authors: RL
authors: Fischer
authors: Olivier
authors: O
authors: Voinnet
authors: Lionel
authors: L
authors: Navarro
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D019175:DNA Methylation; D018744:DNA, Plant / Q000235:genetics* / Q000378:metabolism*; D017343:Genes, Plant; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D009154:Mutation; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D018515:Plant Leaves / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D044224:Pseudomonas syringae / Q000276:immunology / Q000472:pathogenicity; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D018744:DNA, Plant; D034741:RNA, Small Interfering; D011494:Protein Kinases; C411853:FLS2 protein, Arabidopsis