title: Comprehensive analysis of silencing mutants reveals complex regulation of the Arabidopsis methylome.
abstract: Cytosine methylation is involved in various biological processes such as silencing of transposable elements (TEs) and imprinting. Multiple pathways regulate DNA methylation in different sequence contexts, but the factors that regulate DNA methylation at a given site in the genome largely remain unknown. Here we have surveyed the methylomes of a comprehensive list of 86 Arabidopsis gene silencing mutants by generating single-nucleotide resolution maps of DNA methylation. We find that DNA methylation is site specifically regulated by different factors. Furthermore, we have identified additional regulators of DNA methylation. These data and analyses will serve as a comprehensive community resource for further understanding the control of DNA methylation patterning.
authors: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
authors: Hume
authors: H
authors: Stroud
authors: Maxim V C
authors: MV
authors: Greenberg
authors: Suhua
authors: S
authors: Feng
authors: Yana V
authors: YV
authors: Bernatavichute
authors: Steven E
authors: SE
authors: Jacobsen
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000378:metabolism; D018899:CpG Islands; D019175:DNA Methylation; D020868:Gene Silencing; D018745:Genome, Plant; D055106:Genome-Wide Association Study; D006657:Histones / Q000378:metabolism; D034622:RNA Interference; D012319:RNA Polymerase II / Q000378:metabolism; D000072260:RNA Splicing Factors
chemical_list: D029681:Arabidopsis Proteins; C518085:At2g20020 protein, Arabidopsis; D006657:Histones; D000072260:RNA Splicing Factors; D012319:RNA Polymerase II