title: Assessment of tumor antigen-loaded solid lipid nanoparticles as an efficient delivery system for dendritic cell engineering.
abstract: OBJECTIVE
  The work attempts to overcome tumor-associated immune tolerance using a surface-modified solid lipid nanoparticle (SLNP) delivery system for dendritic cell (DC) immunotherapy.
  
  
  METHODS
  Different formulations of SLNPs (SLNPs-alone, cationic SLNPs and mannosylated SLNPs) were prepared using tumor cell lysates. Prepared nanoparticles were characterized and their ability to activate DCs to induce a tumor cell-specific response was assessed.
  
  
  RESULTS
  SLNPs induced a strong phagocytic signal to DCs without any significant toxicity. Comparatively, mannosylated SLNPs evoked an optimum and effective cell-mediated immune response with no significant toxicity.
  
  
  CONCLUSIONS
  Surface-modified SLNPs may play a pivotal role in designing a clinically translatable DC-based immunotherapy for gastrointestinal malignancies. This novel approach may also facilitate the treatment of residual disease, following standard therapy.
authors: Department of Biotechnology, Dr Hari Singh Gour Central University, Sagar, India.
authors: Arpit
authors: A
authors: Bhargava
authors: Dinesh
authors: D
authors: Mishra
authors: Saba
authors: S
authors: Khan
authors: Subodh K
authors: SK
authors: Varshney
authors: Smita
authors: S
authors: Banerjee
authors: Pradyumna K
authors: PK
authors: Mishra
mesh_terms: D000951:Antigens, Neoplasm / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism*; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D005767:Gastrointestinal Diseases / Q000628:therapy; D006801:Humans; D007167:Immunotherapy; D008055:Lipids / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010587:Phagocytosis / Q000502:physiology; D014961:X-Ray Diffraction
chemical_list: D000951:Antigens, Neoplasm; D008055:Lipids