title: Melatonin improved rat cardiac mitochondria and survival rate in septic heart injury.
abstract: The pathogenesis of septic myocardial depression is complicated. Mitochondrial dysfunction has been suggested to be one of the main reasons for the reduced cardiac function. As melatonin is an antioxidant with the potential to scavenge radicals in mitochondria, we therefore employed a sepsis model, that is, cecal ligation and double puncture (CLP) in rats, to study the melatonin effects on: (i), myocardial mitochondrial function; (ii), heart systolic function; and (iii), prognosis of septic rats. We demonstrate that melatonin treatment (30 mg/kg, 3, 6, 12, 18, 24 hr after CLP) (i) improved myocardial cytochrome c oxidase (CcOX) activity and blood lactate level, (ii) attenuated heart dysfunction with a higher left ventricular ejection fraction (EF), and (iii) promoted 48-h survival of the rats compared to CLP animals with no melatonin treatment. In conclusion, our results show that rat myocardial mitochondrial CcOX activity was depressed during severe sepsis accompanied by myocardial depression characterized by the decline of EF. In septic rats, melatonin increased the CcOX activity, improved heart systolic function, and lowered mortality rate. The clinical use of melatonin in septic myocardial depression should be tested in the future.
authors: Department of Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors: Hongmin
authors: H
authors: Zhang
authors: Dawei
authors: D
authors: Liu
authors: Xiaoting
authors: X
authors: Wang
authors: Xiukai
authors: X
authors: Chen
authors: Yun
authors: Y
authors: Long
authors: Wenzhao
authors: W
authors: Chai
authors: Xiang
authors: X
authors: Zhou
authors: Xi
authors: X
authors: Rui
authors: Qing
authors: Q
authors: Zhang
authors: Hao
authors: H
authors: Wang
authors: Quanhui
authors: Q
authors: Yang
mesh_terms: D000818:Animals; D003576:Electron Transport Complex IV / Q000378:metabolism; D006321:Heart / Q000187:drug effects* / Q000503:physiopathology; D053208:Kaplan-Meier Estimate; D019344:Lactic Acid / Q000097:blood; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D008928:Mitochondria / Q000187:drug effects*; D009206:Myocardium / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000503:physiopathology*
chemical_list: D019344:Lactic Acid; D003576:Electron Transport Complex IV; D008550:Melatonin
keywords: melatonin; mitochondrial function; mitochondrial resuscitation; myocardial depression