title: Enhanced performance in the determination of ibuprofen 1-β-O-acyl glucuronide in urine by combining high field asymmetric waveform ion mobility spectrometry with liquid chromatography-time-of-flight mass spectrometry.
abstract: The incorporation of a chip-based high field asymmetric waveform ion mobility spectrometry (FAIMS) separation in the ultra (high)-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS) determination of the (R/S) ibuprofen 1-β-O-acyl glucuronide metabolite in urine is reported. UHPLC-FAIMS-HRMS reduced matrix chemical noise, improved the limit of quantitation approximately two-fold and increased the linear dynamic range compared to the determination of the metabolite without FAIMS separation. A quantitative evaluation of the prototype UHPLC-FAIMS-HRMS system showed better reproducibility for the drug metabolite (%RSD 2.7%) at biologically relevant concentrations in urine. In-source collision induced dissociation of the FAIMS-selected deprotonated metabolite was used to fragment the ion prior to mass analysis, enhancing selectivity by removing co-eluting species and aiding the qualitative identification of the metabolite by increasing the signal-to-noise ratio of the fragment ions.
authors: Centre for Analytical Science, Department of Chemistry, Loughborough University, Leicestershire, LE11 3TU, UK.
authors: Robert W
authors: RW
authors: Smith
authors: Danielle E
authors: DE
authors: Toutoungi
authors: James C
authors: JC
authors: Reynolds
authors: Anthony W T
authors: AW
authors: Bristow
authors: Andrew
authors: A
authors: Ray
authors: Ashley
authors: A
authors: Sage
authors: Ian D
authors: ID
authors: Wilson
authors: Daniel J
authors: DJ
authors: Weston
authors: Billy
authors: B
authors: Boyle
authors: Colin S
authors: CS
authors: Creaser
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005965:Glucuronates / Q000652:urine*; D006801:Humans; D007052:Ibuprofen / Q000031:analogs & derivatives* / Q000652:urine; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D005965:Glucuronates; C081281:ibuprofen acyl glucuronide; D007052:Ibuprofen