title: Antimicrobial activity of the synthetic peptide Lys-a1 against oral streptococci.
abstract: The peptide LYS-[TRP(6)]-Hy-A1 (Lys-a1) is a synthetic derivative of the peptide Hy-A1, initially isolated from the frog species Hypsiboas albopunctatus. According to previous research, it is a molecule with broad antimicrobial activity. The objective of this study was to evaluate the antimicrobial activity of the synthetic peptide Lys-a1 (KIFGAIWPLALGALKNLIK-NH2) on the planktonic and biofilm growth of oral bacteria. The methods used to evaluate antimicrobial activity include the following: determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) in microtiter plates for growth in suspension and quantification of biomass by crystal violet staining and counting of colony forming units for biofilm growth. The microorganisms Streptococcus oralis, Streptococcus sanguinis, Streptococcus parasanguinis, Streptococcus salivarius, Streptococcus mutans and Streptococcus sobrinus were grown in Brain Heart Infusion broth at 37°C under atmospheric pressure with 10% CO2. The peptide was solubilized in 0.1% acetic acid (v/v) at various concentrations (500-1.9 μg mL(-1)). Chlorhexidine gluconate 0.12% was used as the positive control, and BHI culture medium was used as the negative control. The tested peptide demonstrated a remarkable antimicrobial effect, inhibiting the planktonic and biofilm growth of all strains tested, even at low concentrations. Thus, the peptide Lys-a1 is an important source for potential antimicrobial agents, especially for the control and prevention of microbial biofilms, which is one of the most important factors in cariogenic processes.
authors: BioMol Group/DPML/LIBS, Integrate Biomolecules Laboratory, Federal University of Ceara, Fortaleza, CE, Brazil.
authors: Bruno Rocha
authors: BR
authors: da Silva
authors: Victor Aragão Abreu
authors: VA
authors: de Freitas
authors: Victor Alves
authors: VA
authors: Carneiro
authors: Francisco Vassiliepe Sousa
authors: FV
authors: Arruda
authors: Esteban Nicolás
authors: EN
authors: Lorenzón
authors: Andréa Silvia Walter
authors: AS
authors: de Aguiar
authors: Eduardo Maffud
authors: EM
authors: Cilli
authors: Benildo Sousa
authors: BS
authors: Cavada
authors: Edson Holanda
authors: EH
authors: Teixeira
mesh_terms: D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000737:chemistry; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D015169:Colony Count, Microbial; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009055:Mouth / Q000382:microbiology; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D013291:Streptococcus / Q000187:drug effects*; D013295:Streptococcus mutans / Q000187:drug effects; D013298:Streptococcus sanguis / Q000187:drug effects; D016986:Streptococcus sobrinus / Q000187:drug effects
chemical_list: D029845:Amphibian Proteins; D000890:Anti-Infective Agents; D010455:Peptides; C558935:hylin-a1 protein, Hypsiboas albopunctatus