title: TNF-alpha single nucleotide polymorphisms in atopic dermatitis.
abstract: Tumor necrosis factor-alpha (TNF-α) could be considered as potential biomarkers in atopic dermatitis (AD), while its level could be influenced by cytokine single gene polymorphisms (SNP). This study was performed in 89 pediatric patients with AD and 137 controls to assess polymorphisms of the TNF-α gene at positions -308 and -238, using the polymerase chain reaction and the sequence-specific primers method. The highest positive allelic association that made the patients susceptible to AD was seen for TNF-α -238/G (p<0.001) and TNF-α -308/G (p = 0.003). The GG genotypes at TNF-α -238 and TNF-α -308, were both significantly higher in the patients with AD, compared to the controls (p<0.01). The GG haplotype at TNF-α (-308,-238) was seen in 92.7% of the patients, which was significantly higher than the controls (p<0.001), while a negative haplotypic association with AD was seen for TNF-α (-308, -238) AG and GA (p<0.01). This study showed that the AG genotype of TNF-α -308, associated with a high production of cytokines, was significantly decreased in patients with AD, while the low-producing GG genotype, which could lead to low production of TNF-α, was over-expressed in the atopic patients.
authors: Pediatrics Center of Excellence, Children's Medical Center.
authors: Nasrin
authors: N
authors: Behniafard
authors: Mohammad
authors: M
authors: Gharagozlou
authors: Elham
authors: E
authors: Farhadi
authors: Mojdeh
authors: M
authors: Khaledi
authors: Soheila
authors: S
authors: Sotoudeh
authors: Behzad
authors: B
authors: Darabi
authors: Seid Mohammad
authors: SM
authors: Fathi
authors: Zahra
authors: Z
authors: Gholizadeh Moghaddam
authors: Mahdi
authors: M
authors: Mahmoudi
authors: Asghar
authors: A
authors: Aghamohammadi
authors: Ali Akbar
authors: AA
authors: Amirzargar
authors: Nima
authors: N
authors: Rezaei
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D003876:Dermatitis, Atopic / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007492:Iran; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: C439524:TNF protein, human; D014409:Tumor Necrosis Factor-alpha