title: Chrysoporthe cubensis: a new source of cellulases and hemicellulases to application in biomass saccharification processes.
abstract: The plant pathogenic fungus Chrysoporthe cubensis was cultivated under solid state employing different substrates and the highest endoglucanase (33.84Ug(-1)), FPase (2.52Ug(-1)), β-glucosidase (21.55Ug(-1)) and xylanase (362.38Ug(-1)) activities were obtained using wheat bran as carbon source. Cellulases and xylanase produced by C. cubensis showed maximal hydrolysis rate at pH 4.0 and in a temperature range of 50-60°C. All enzymatic activities were highly stable at 40 and 50°C through 48h of pre-incubation. Saccharification of alkaline pretreated sugarcane bagasse by crude enzyme extract from C. cubensis resulted in release of 320.8mg/g and 288.7mg/g of glucose and xylose, respectively. On another hand, a similar assay employing commercial cellulase preparation resulted in release of 250.6mg/g and 62.1mg/g of glucose and xylose, respectively. Cellulolytic extract from C. cubensis showed a great potential to be used in biomass saccharification processes.
authors: Department of Biochemistry and Molecular Biology, Federal University of Viçosa, Viçosa, Minas Gerais 36570-000, Brazil.
authors: Daniel Luciano
authors: DL
authors: Falkoski
authors: Valéria Monteze
authors: VM
authors: Guimarães
authors: Maíra Nicolau
authors: MN
authors: de Almeida
authors: Acelino Couto
authors: AC
authors: Alfenas
authors: Jorge Luiz
authors: JL
authors: Colodette
authors: Sebastião Tavares
authors: ST
authors: de Rezende
mesh_terms: D001203:Ascomycota / Q000201:enzymology*; D018533:Biomass; D044602:Cellulases / Q000378:metabolism*; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D014995:Xylosidases / Q000378:metabolism
chemical_list: D044602:Cellulases; D006026:Glycoside Hydrolases; D014995:Xylosidases; C023305:hemicellulase