title: Characterization and genome sequencing of phage Abp1, a new phiKMV-like virus infecting multidrug-resistant Acinetobacter baumannii.
abstract: While screening for alternative antibiotics against multidrug-resistant Acinetobacter baumannii, we isolated a virulent A. baumannii bacteriophage Abp1. Transmission electron microscopy revealed that the phage had an icosahedral head with a short tail and should be classified as a member of the Podoviridae family. SDS-PAGE showed that Abp1 contained at least one major and nine minor proteins. In a single-step growth test, we demonstrated that Abp1 had a latent period of 10 min and a burst size of 350. Abp1 also had a relatively narrow host range. The entire genome was sequenced, and the final assembly yielded a 42,185 bp, linear, double-stranded DNA molecule with a G+C content of 39.15 % and containing 54 putative genes. Among these genes, 26 were functionally known, leaving 28 unknown putative genes. Abp1 is a new member of the phiKMV-like virus subgroup of the T7 group; its genome sequence is very similar to that of the A. baumannii phage phiAB1.
authors: Department of Microbiology, Third Military Medical University, Chongqing 400038, China.
authors: Guangtao
authors: G
authors: Huang
authors: Shuai
authors: S
authors: Le
authors: Yizhi
authors: Y
authors: Peng
authors: Yan
authors: Y
authors: Zhao
authors: Supeng
authors: S
authors: Yin
authors: Lin
authors: L
authors: Zhang
authors: Xinyue
authors: X
authors: Yao
authors: Yinling
authors: Y
authors: Tan
authors: Ming
authors: M
authors: Li
authors: Fuquan
authors: F
authors: Hu
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000302:isolation & purification / Q000821:virology*; D001482:Base Composition / Q000235:genetics; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D016679:Genome, Viral; D058507:Host Specificity; D008969:Molecular Sequence Data; D010802:Phylogeny; D017902:Podoviridae / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins