title: Effects of selected ionic liquids on lipid production by the oleaginous yeast Rhodosporidium toruloides.
abstract: Lignocellulosic biomass pretreatment with ionic liquids (ILs) has been emerged as a new technology, but the effects of residual ILs on the downstream biotransformation remain largely unknown. Here, three typical ILs were tested for their effects on lipid production by the oleaginous yeast Rhodosporidium toruloides AS 2.1389. When cultures were maintained at pH 6.0 in the presence of 30mM ILs, [Emim]Cl, [Emim][DEP], or [Emim][OAc], minor inhibition effects were observed. When cultures were performed in the presence of 60mM ILs or without pH control, inhibition was largely dependent on ILs. Detailed analysis indicated that the anion of [Emim][OAc] was assimilated, leading to a rapid alkaline-pH shift and enhanced inhibition on cell growth and lipid production. Our results demonstrated that R. toruloides is a robust lipid producer tolerating ILs at low concentrations, and that care should be taken in bioprocess control and data analysis when ILs are involved.
authors: School of Food Science and Technology, Dalian Ploytechnic University, Dalian 116034, PR China.
authors: Qitian
authors: Q
authors: Huang
authors: Qian
authors: Q
authors: Wang
authors: Zhiwei
authors: Z
authors: Gong
authors: Guojie
authors: G
authors: Jin
authors: Hongwei
authors: H
authors: Shen
authors: Shan
authors: S
authors: Xiao
authors: Haibo
authors: H
authors: Xie
authors: Shuhong
authors: S
authors: Ye
authors: Jihui
authors: J
authors: Wang
authors: Zongbao K
authors: ZK
authors: Zhao
mesh_terms: D056804:Biofuels; D018533:Biomass; D052578:Ionic Liquids / Q000633:toxicity*; D050356:Lipid Metabolism; D015003:Yeasts / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
chemical_list: D056804:Biofuels; D052578:Ionic Liquids