title: Enzymatic degradation of polyester films by a cutinase-like enzyme from Pseudozyma antarctica: surface plasmon resonance and atomic force microscopy study.
abstract: Enzymatic degradation of polyester films by a cutinase-like enzyme from Pseudozyma antarctica JCM10317 (PaE) was analyzed by surface plasmon resonance (SPR). The adsorption of PaE and the degradation rate for polyester films were quantitatively monitored by a positive and negative SPR signal shifts, respectively. The decrease in SPR signal and the erosion depth of amorphous poly(L-lactide) (a-PLLA) film measured by atomic force microscopy (AFM) had a linear relationship, and the weight loss was estimated from the AFM data combined with a density of a-PLLA film. Furthermore, SPR sensorgrams for various polyester films showed that degradation rate of poly(ε-caprolactone) and poly(butylene succinate-co-adipate) which contain C6 units was higher than that of other polyesters such as poly(butylene succinate) and a-PLLA. These results suggest that C6 is the preferred chain length as substrates for PaE.
authors: National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Tsukuba, Ibaraki, 305-8604, Japan.
authors: Yukiko
authors: Y
authors: Shinozaki
authors: Yoshihiro
authors: Y
authors: Kikkawa
authors: Shun
authors: S
authors: Sato
authors: Tokuma
authors: T
authors: Fukuoka
authors: Takashi
authors: T
authors: Watanabe
authors: Shigenobu
authors: S
authors: Yoshida
authors: Toshiaki
authors: T
authors: Nakajima-Kambe
authors: Hiroko K
authors: HK
authors: Kitamoto
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D011091:Polyesters / Q000378:metabolism*; D013379:Substrate Specificity; D020349:Surface Plasmon Resonance; D014587:Ustilaginales / Q000201:enzymology*
chemical_list: D011091:Polyesters; D002265:Carboxylic Ester Hydrolases; C019952:cutinase