title: Photochemoprotective effect of Calluna vulgaris extract on skin exposed to multiple doses of ultraviolet B in SKH-1 hairless mice.
abstract: UVB is a major cause of nonmelanoma skin cancer in humans. Photochemoprevention represents an important strategy in protecting the skin against the detrimental effects of ultraviolet B (UVB). We investigated the activity of Calluna vulgaris (Cv) delivered via a hydrogel on 3 main pathways (oxidative stress, inflammation, DNA damage) on skin exposed to multiple doses of UVB in SKH-1 mice. Fifty female mice were divided randomly into 5 groups: control, vehicle, UVB irradiated, Cv + UVB irradiated, and Cv + vehicle + UVB irradiated. The extract was applied topically on the skin in a dose of 4 mg polyphenols/cm2 30 minutes before each UVB (240 mJ/cm2) exposure over 10 consecutive days. Malondialdehyde, reduced glutathione, tumor necrosis factor-α, interleukin-6, cyclobutane pyrimidine dimer (CPD) levels, sunburn cell formation and epidermal thickness, and the number of epidermal cell layers in skin were evaluated 24 hours after the last treatment. UVB increased cytokine levels (P < 0.001), formation of CPDs (P < 0.001) and sunburn cells (P < 0.001), and the epidermal thickness and number of epidermal cell layers (P < 0.001) compared with the control group. The topical application of Cv protected the skin against inflammation and DNA damage, as shown by a decreased number of CPDs (P < 0.001) and sunburn cells (P < 0.001). The administration of Cv via hydrogel may be a viable method for chemoprevention..
authors: Department of Physiology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors: Elena Diana
authors: ED
authors: Olteanu
authors: Adriana
authors: A
authors: Filip
authors: Simona
authors: S
authors: Clichici
authors: Doina
authors: D
authors: Daicoviciu
authors: Marcela
authors: M
authors: Achim
authors: Ion Dan
authors: ID
authors: Postescu
authors: Pompei
authors: P
authors: Bolfa
authors: Laura
authors: L
authors: Bolojan
authors: Laurian
authors: L
authors: Vlase
authors: Adriana
authors: A
authors: Muresan
mesh_terms: D000818:Animals; D029795:Calluna / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D016207:Cytokines; D004195:Disease Models, Animal; D005260:Female; D016166:Free Radical Scavengers / Q000032:analysis; D006801:Humans; D013058:Mass Spectrometry; D051379:Mice; D008812:Mice, Hairless; D018384:Oxidative Stress; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology; D059808:Polyphenols / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects*; D012878:Skin Neoplasms / Q000517:prevention & control*; D013471:Sunburn / Q000150:complications* / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D016207:Cytokines; D016166:Free Radical Scavengers; D010936:Plant Extracts; D059808:Polyphenols