title: Strategies for outcrossing and genetic manipulation of Drosophila compound autosome stocks.
abstract: Among all organisms, Drosophila melanogaster has the most extensive well-characterized collection of large-scale chromosome rearrangements. Compound chromosomes, rearrangements in which homologous chromosome arms share a centromere, have proven especially useful in genetic-based surveys of the entire genome. However, their potential has not been fully realized because compound autosome stocks are refractile to standard genetic manipulations: if outcrossed, they yield inviable aneuploid progeny. Here we describe two strategies, cold-shock and use of the bubR1 mutant alleles, to produce nullo gametes through nondisjunction. These gametes are complementary to the compound chromosome-bearing gametes and thus produce viable progeny. Using these techniques, we created a compound chromosome two C(2)EN stock bearing a red fluorescent protein-histone transgene, facilitating live analysis of these unusually long chromosomes.
authors: Instituto de Biologia Molecular e Celular, Universidade do Porto, 4150-180 Porto, Portugal.
authors: T
authors: T
authors: Martins
authors: S
authors: S
authors: Kotadia
authors: N
authors: N
authors: Malmanche
authors: C E
authors: CE
authors: Sunkel
authors: W
authors: W
authors: Sullivan
mesh_terms: D000818:Animals; D001947:Breeding / Q000379:methods*; D058639:Cold-Shock Response; D003433:Crosses, Genetic; D020732:Cytogenetic Analysis / Q000379:methods; D004331:Drosophila melanogaster / Q000235:genetics*; D005718:Gametogenesis / Q000235:genetics / Q000502:physiology*; D018014:Gene Transfer Techniques; D008164:Luminescent Proteins / Q000235:genetics; D009630:Nondisjunction, Genetic / Q000235:genetics*; D014178:Translocation, Genetic / Q000235:genetics*; D000097573:Red Fluorescent Protein
chemical_list: D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein
keywords: C(2)EN; HisH2Av-mRFP1; long chromosome; mitosis; neuroblast