title: Conversion of rat embryonic stem cells into neural precursors in chemical-defined medium.
abstract: Rat embryonic stem (ES) cells hold great interest for the research of neurodevelopment and neurodegenerative diseases. However, neural conversion of rat ES cells in vitro has proven to be a challenge owing to the proliferation arrest and apoptosis. Here we report that rat ES cells can commit efficiently to a neural fate in the presence of CHIR99021 and Y-27632 (CY medium). In addition, CHIR99021 is crucial for maintaining the metabolic activity of differentiated rat ES cells, while Y-27632 facilitates the neural differentiation of rat ES cells by inhibiting bone morphogenetic protein expression. The chemical-defined CY medium also provides a platform for exploring the mechanism of neural commitment and optimizing the production efficiency of neural precursor from rat ES cells.
authors: Laboratory of Viral and Gene Therapy, Eastern Hepatobiliary Surgical Hospital, The Second Military Medical University, Shanghai, PR China.
authors: Xinrong
authors: X
authors: Peng
authors: Haixia
authors: H
authors: Gao
authors: Ying
authors: Y
authors: Wang
authors: Baotian
authors: B
authors: Yang
authors: Tao
authors: T
authors: Liu
authors: Yan
authors: Y
authors: Sun
authors: Huajun
authors: H
authors: Jin
authors: Lihua
authors: L
authors: Jiang
authors: Linfang
authors: L
authors: Li
authors: Mengchao
authors: M
authors: Wu
authors: Qijun
authors: Q
authors: Qian
mesh_terms: D000577:Amides / Q000494:pharmacology; D000818:Animals; D055415:Bone Morphogenetic Protein 4 / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D053595:Embryonic Stem Cells / Q000187:drug effects*; D038362:Glycogen Synthase Kinase 3 / Q000037:antagonists & inhibitors; D058953:Neural Stem Cells / Q000166:cytology*; D055495:Neurogenesis / Q000187:drug effects* / Q000502:physiology; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D051381:Rats
chemical_list: D000577:Amides; D055415:Bone Morphogenetic Protein 4; C473711:Chir 99021; D003470:Culture Media; D047428:Protein Kinase Inhibitors; D011725:Pyridines; D011743:Pyrimidines; C108830:Y 27632; D038362:Glycogen Synthase Kinase 3