title: Construction of an integrated enzyme system consisting azoreductase and glucose 1-dehydrogenase for dye removal.
abstract: Azo dyes are toxic and carcinogenic and are often present in industrial effluents. In this research, azoreductase and glucose 1-dehydrogenase were coupled for both continuous generation of the cofactor NADH and azo dye removal. The results show that 85% maximum relative activity of azoreductase in an integrated enzyme system was obtained at the conditions: 1U azoreductase:10U glucose 1-dehydrogenase, 250mM glucose, 1.0mM NAD(+) and 150μM methyl red. Sensitivity analysis of the factors in the enzyme system affecting dye removal examined by an artificial neural network model shows that the relative importance of enzyme ratio between azoreductase and glucose 1-dehydrogenase was 22%, followed by dye concentration (27%), NAD(+) concentration (23%) and glucose concentration (22%), indicating none of the variables could be ignored in the enzyme system. Batch results show that the enzyme system has application potential for dye removal.
authors: Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China.
authors: Yuyi
authors: Y
authors: Yang
authors: Buqing
authors: B
authors: Wei
authors: Yuhua
authors: Y
authors: Zhao
authors: Jun
authors: J
authors: Wang
mesh_terms: D001391:Azo Compounds / Q000302:isolation & purification*; D004396:Coloring Agents / Q000302:isolation & purification*; D005947:Glucose / Q000378:metabolism; D042843:Glucose 1-Dehydrogenase / Q000378:metabolism*; D008956:Models, Chemical; D009243:NAD / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D016571:Neural Networks, Computer; D009601:Nitroreductases; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D014874:Water Pollutants, Chemical; D009243:NAD; C008492:methyl red; D042843:Glucose 1-Dehydrogenase; D009247:NADH, NADPH Oxidoreductases; D009601:Nitroreductases; C021106:azoreductase; D005947:Glucose