title: Isolation and DNA characterization of a simian retrovirus 5 from a Japanese monkey (Macaca fuscata).
abstract: An SRV-like virus was isolated from a colony-born Japanese monkey. To identify this SRV-like virus, we designed universal primers at regions that were conserved among the reported SRV sequences in the 5'-LTR and the short ORF and we obtained plasmid clones containing the complete gag, prt, pol and env genes. The full-length sequences of the isolate were determined from the plasmids and by direct sequencing. Sequence comparisons and phylogenetic analyses indicated that this SRV-like virus had a sequence identical to the reported 626 bp of SRV-5. In this study, we isolated SRV5/JPN/2005/V1 from a Japanese monkey and characterized the full-length SRV-5 sequence.
authors: The Corporation for Production and Research of Laboratory Primates, 1-16-2 Sakura, Tsukuba, Ibaraki 305-0003, Japan.|Tsukuba Primate Research Center, National Institute of Biomedical Innovation, 1-1 Hachimandai, Tsukuba, Ibaraki 305-0843, Japan.
authors: Jun-Ichiro
authors: JI
authors: Takano
authors: BioReliance Corporation, 9630 Medical Center Drive, Rockville, MD 20850, USA.
authors: Arlene
authors: A
authors: Leon
authors: The Corporation for Production and Research of Laboratory Primates, 1-16-2 Sakura, Tsukuba, Ibaraki 305-0003, Japan.
authors: Miyoko
authors: M
authors: Kato
authors: Amami Wild Animal Research Center Inc., Tatsugo, Amami, Kagoshima 894-0105, Japan.
authors: Yuko
authors: Y
authors: Abe
authors: The Corporation for Production and Research of Laboratory Primates, 1-16-2 Sakura, Tsukuba, Ibaraki 305-0003, Japan.
authors: Koji
authors: K
authors: Fujimoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics*; D015752:Genes, env / Q000235:genetics; D015750:Genes, gag / Q000235:genetics; D015751:Genes, pol / Q000235:genetics; D016679:Genome, Viral / Q000235:genetics*; D007564:Japan; D008251:Macaca; D008969:Molecular Sequence Data; D008992:Monkey Diseases / Q000821:virology*; D010802:Phylogeny; D012192:Retroviridae Infections / Q000821:virology*; D015301:Retroviruses, Simian / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D004279:DNA, Viral