title: [Study on HPLC fingerprint of Prunella vulgaris].
abstract: OBJECTIVE
  To establish HPLC fingerprint of Prunella vulgaris and provide evidence for quality control and identification of the Chinese crude drug.
  
  
  METHODS
  The HPLC method was used, chromatography conditions were Agilent Eclipse XDB-C18 (4.6 mm x 250 mm, 5 microm) column with gradient mobile phase of acetonitrile-0.1% acetic acid, UV detection wavelength was 290 nm and the column temperature was 30 degrees C with the flow rate of 1.0 mL/min, the sample injection was 10 microL.
  
  
  RESULTS
  HPLC fingerprints of 19 samples of Prunella vulgaris were established. 14 common peaks were selected as the fingerprint peaks in all samples. Among the obtained fingerprints, most of the detected peaks were separated effectively. 19 samples had high similarities.
  
  
  CONCLUSIONS
  The established HPLC fingerprint has desirable accuracy, repeatability and stability, which can be used for one of the quality control methods of Prunella vulgaris.
authors: Guangzhou Baiyunshan Xingqun Pharmaceutical Co., Ltd., Guangzhou 510288, China. xuzd1688@yahoo.com.cn
authors: Zhao-Dong
authors: ZD
authors: Xu
authors: Jiang-Xiong
authors: JX
authors: Yao
authors: Ju-Yan
authors: JY
authors: Liu
authors: Chun
authors: C
authors: Li
authors: Li-Mei
authors: LM
authors: Lin
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002934:Cinnamates / Q000032:analysis; D053630:Depsides / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D031347:Prunella / Q000737:chemistry*; D011786:Quality Control; D000097123:Rosmarinic Acid
chemical_list: D002934:Cinnamates; D053630:Depsides; D004365:Drugs, Chinese Herbal; D000097123:Rosmarinic Acid