title: Occurrence of alternariol and alternariol monomethyl ether in beverages from the Entre Rios Province market, Argentina.
abstract: One hundred and eighty five samples of red, white and rosé wines and different juices purchased in Entre Rios, Argentina, were analyzed for the Alternaria mycotoxins alternariol (AOH) and alternariol methyl ether (AME). White wines were analyzed after removal of alcohol by a nitrogen stream and concentrated. AOH in red wines was cleaned up by solid-phase extraction columns in series (octadecyl and amino propyl modified silica) and AME quantified directly on the sample. The juices were filtered and concentrated, and then all sample extracts were quantified by high performance liquid chromatography with photodiode array detector that allows confirmation through UV spectra. Method validation revealed a good sensitivity with adequate LOD and LOQ for AME and less sensitivity for AOH (i.e. white wine: AME 0.8 and 1.4 ng/mL, AOH 2 and 3.3 ng/mL; red wine: AME 0.1 and 0.2 ng/mL, AOH 4.5 and 7.5 ng/mL; apple juice: AME 1.7 and 2.8 ng/mL, AOH 5 and 9 ng/mL; other juices: AME 2.0 and 3.1 ng/mL, AOH 6 and 10 ng/mL). Recoveries in all cases were greater than 80 %. Four of 53 white wine samples were contaminated with AOH with a maximum level of 18 ng/mL, 6 of 56 samples of red wine had a maximum of 13 ng/mL, and 3 of 68 samples of juices had traces of AOH. AME was less frequently detected than AOH, and the LOD and LOQ for AME are smaller than for AOH. Only three samples of white wine and one of red wine were contaminated, but in only one white wine sample (AME 225 ng/mL) did the toxin level exceed the LOQ.
authors: Facultad de Bromatología, Universidad Nacional de Entre Ríos, Perón 64, 2820, Gualeguaychú, Entre Ríos, Argentina.
authors: Leticia
authors: L
authors: Broggi
authors: Cora
authors: C
authors: Reynoso
authors: Silvia
authors: S
authors: Resnik
authors: Fernanda
authors: F
authors: Martinez
authors: Vanesa
authors: V
authors: Drunday
authors: Angela Romero
authors: AR
authors: Bernal
mesh_terms: D001118:Argentina; D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007783:Lactones / Q000032:analysis*; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods
chemical_list: D007783:Lactones; C005197:alternariol; C018206:alternariol monomethyl ether