title: Oct-4 is required for an antiapoptotic behavior of chemoresistant colorectal cancer cells enriched for cancer stem cells: effects associated with STAT3/Survivin.
abstract: Cancer stem cells (CSCs) have been implicated in multidrug resistance, a phenomenon responsible for the failure of cancer chemotherapy. However, the underlying mechanisms are still unclear. In our study, we established two oxaliplatin-resistant colorectal cancer cell lines displaying some CSCs characteristics. Oct4 overexpression was observed in these two lines. We performed Oct4 knock down by lentiviral vector-mediated specific shRNA. Knockdown increased apoptosis, decreased CSCs marker expression and weakened tumorigenicity in drug-resistant cell lines. In conclusion, we show that these events can be at least in part attributed to the STAT3/Survivin pathway.
authors: Department of General Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors: Kunming
authors: K
authors: Wen
authors: Zhongxue
authors: Z
authors: Fu
authors: Xingye
authors: X
authors: Wu
authors: Jihong
authors: J
authors: Feng
authors: Wangsheng
authors: W
authors: Chen
authors: Jiang
authors: J
authors: Qian
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D017209:Apoptosis; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000473:pathology; D019008:Drug Resistance, Neoplasm; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis; D051034:Inhibitor of Apoptosis Proteins / Q000502:physiology*; D051379:Mice; D016513:Mice, SCID; D014411:Neoplastic Stem Cells / Q000737:chemistry / Q000502:physiology*; D050814:Octamer Transcription Factor-3 / Q000502:physiology*; D009944:Organoplatinum Compounds / Q000494:pharmacology; D000077150:Oxaliplatin; D010455:Peptides / Q000032:analysis; D050796:STAT3 Transcription Factor / Q000502:physiology*; D000077022:Survivin
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; C107489:BIRC5 protein, human; D006023:Glycoproteins; D018960:Hyaluronan Receptors; D051034:Inhibitor of Apoptosis Proteins; D050814:Octamer Transcription Factor-3; D009944:Organoplatinum Compounds; C494173:POU5F1 protein, human; D010455:Peptides; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D000077022:Survivin; D000077150:Oxaliplatin