title: Optical imaging of HPV infection in a murine model.
abstract: The development of animal models of HPV infection has given investigators a new set of tools to expand basic knowledge of the early events of infection in vivo. The use of HPV pseudovirions, in which the viral genome has been replaced with a reporter pseudogenome, in combination with advanced imaging techniques has facilitated and simplified studies using these models. Herein we provide details for a murine model of cervicovaginal HPV infection in conjunction with several methods for imaging and quantitating the transduced genes, both ex vivo and in vivo.
authors: Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. rkines@aurabiosciences.com
authors: Rhonda C
authors: RC
authors: Kines
authors: Hisataka
authors: H
authors: Kobayashi
authors: Peter L
authors: PL
authors: Choyke
authors: Marcelino L
authors: ML
authors: Bernardo
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D017930:Genes, Reporter; D006801:Humans; D049449:Luminescence; D051379:Mice; D008807:Mice, Inbred BALB C; D061848:Optical Imaging / Q000379:methods*; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000235:genetics / Q000473:pathology*; D014161:Transduction, Genetic; D014621:Vagina / Q000473:pathology* / Q000821:virology; D014771:Virion / Q000235:genetics*