title: Microbacterium saccharophilum sp. nov., isolated from a sucrose-refining factory.
abstract: A Gram-stain-positive, non-motile, non-spore-forming, rod-shaped bacterium, designated strain K-1(T), was isolated from soil at a sucrose refinery in Japan. The strain grew at 9-37 °C (optimum, 30 °C) and at pH 6-11 (optimum, pH 7.0). Phylogenetic analysis based on the full-length 16S rRNA gene sequence of strain K-1(T) revealed that it was a member of the genus Microbacterium. High 16S rRNA gene sequence similarities were found between strains K-1(T) and both Microbacterium pumilum NBRC 101279(T) (99.7 %) and Microbacterium deminutum NRRL B-24453(T) (99.5 %). However, the DNA-DNA hybridization values between strain K-1(T) and M. pumilum NBRC 101279(T) and M. deminutum NRRL B-24453(T) were only 12 % and 10 %, respectively. The DNA G+C content of strain K-1(T) was 73 mol%. The major fatty acids of strain K-1(T) were anteiso-C15 : 0 and anteiso-C17 : 0, and the major menaquinones were MK-12 and MK-13. The diamino acid in the cell-wall peptidoglycan was lysine. On the basis of these results, strain K-1(T) is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium saccharophilum sp. nov. is proposed. The type strain is K-1(T) (= NBRC 108778(T) = NCIMB 14782(T)).
authors: Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan.
authors: Yukari
authors: Y
authors: Ohta
authors: Ensuiko Sugar Refining Co., Ltd, 1-1-1 Fukuura, Kanazawa-ku, Yokohama-shi, 236-0004, Japan.
authors: Tetsuya
authors: T
authors: Ito
authors: Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan.
authors: Kozue
authors: K
authors: Mori
authors: Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan.
authors: Shinro
authors: S
authors: Nishi
authors: Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan.
authors: Yasuhiro
authors: Y
authors: Shimane
authors: Ensuiko Sugar Refining Co., Ltd, 1-1-1 Fukuura, Kanazawa-ku, Yokohama-shi, 236-0004, Japan.
authors: Katsuhiko
authors: K
authors: Mikuni
authors: Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan.
authors: Yuji
authors: Y
authors: Hatada
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D007564:Japan; D008239:Lysine / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013395:Sucrose / Q000378:metabolism*; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D013395:Sucrose; D008239:Lysine