title: Optimization of clean fractionation process applied to switchgrass to produce pulp for enzymatic hydrolysis.
abstract: The purpose of this study was to fractionate switchgrass (SG) to obtain hemicellulose-, lignin-rich fractions and highly digestible pulp, using a clean fractionation (CF) approach. The main objective was to produce highest glucose yield in the enzymatic hydrolysis of pulp. Effects of processing factors such as time (10-50 min), temperature (120-160 °C), catalyst concentration (0.21-0.93% w/w sulfuric acid) and organic solvent mixture composition (7-43% w/w methyl isobutyl ketone) were evaluated. Response surface methodology and central composite design were used for process optimization and statistical analyses. High lignin (75-93%) and xylan (83-100%) removal from biomass were obtained, leaving solid pulp rich in glucan (78-94%). High enzymatic hydrolysis glucose yields (more than 90%) were obtained for selected optimal conditions. Pulp can be used for ethanol production while separated xylan and lignin fractions can be used as a feedstock for value-added products which suggests the applicability of clean fractionation technology in a biorefinery concept.
authors: Department of Agricultural and Biosystems Engineering, South Dakota State University, 1400 N Campus D, Ag. Eng. Building, Brookings, SD 57007, USA. grzegorz.brudecki@sdstate.edu
authors: Grzegorz
authors: G
authors: Brudecki
authors: Iwona
authors: I
authors: Cybulska
authors: Kurt
authors: K
authors: Rosentrater
mesh_terms: D002480:Cellulase / Q000737:chemistry*; D005591:Chemical Fractionation / Q000379:methods*; D003198:Computer Simulation; D005947:Glucose / Q000138:chemical synthesis*; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry* / Q000302:isolation & purification; D008956:Models, Chemical; D010936:Plant Extracts; D006109:Poaceae / Q000737:chemistry*; D013464:Sulfuric Acids / Q000737:chemistry*; D001617:beta-Glucosidase / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D013464:Sulfuric Acids; C036909:lignocellulose; D008031:Lignin; D001617:beta-Glucosidase; D002480:Cellulase; D005947:Glucose; C033158:sulfuric acid