title: Latex bead-based artificial antigen-presenting cells induce tumor-specific CTL responses in the native T-cell repertoires and inhibit tumor growth.
abstract: Cell-free artificial antigen-presenting cells (aAPCs) were generated by coupling H-2K(b)/TRP2 tetramers together with anti-CD28 and anti-4-1BB antibodies onto cell-sized latex beads and injected intravenously and subcutaneously into naïve mice and antigen-primed mice (B6, H-2K(b)). Vigorous tumor antigen-specific CTL responses in the native T-cell repertoire in each mouse model were elicited as evaluated by measuring surface CD69 and CD25, intracellular IFN-γ, tetramer staining and cytolysis of melanoma cells. Furthermore, the aAPCs efficiently inhibited subcutaneous tumor growth and markedly delayed tumor progression in tumor-bearing mice. These data suggest that bead-based aAPCs represent a potential strategy for the active immunotherapy of cancers or persistent infections.
authors: Department of Microbiology and Immunology, Southeast University Medical School, Nanjing, Jiangsu, China. scl_xia@hotmail.com
authors: Chuanlai
authors: C
authors: Shen
authors: Kai
authors: K
authors: Cheng
authors: Shenwei
authors: S
authors: Miao
authors: Wei
authors: W
authors: Wang
authors: Yong
authors: Y
authors: He
authors: Fanyan
authors: F
authors: Meng
authors: Jianqiong
authors: J
authors: Zhang
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000737:chemistry / Q000276:immunology; D058045:Artificial Cells / Q000276:immunology*; D045744:Cell Line, Tumor; D016207:Cytokines / Q000096:biosynthesis; D008297:Male; D008545:Melanoma / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology; D051379:Mice; D008863:Microspheres; D009369:Neoplasms / Q000276:immunology* / Q000378:metabolism; D010446:Peptide Fragments / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000951:Antigens, Neoplasm; D016207:Cytokines; D008565:Membrane Proteins; D010446:Peptide Fragments; C500290:peptide SVYDFFVWL