title: Determination of viremia and concentration of circulating nonstructural protein 1 in patients infected with dengue virus in Mexico.
abstract: Higher levels of viremia and circulating nonstructural protein 1 (NS1) have been associated with dengue disease severity. In this study, viremia and circulating NS1 levels were determined in 225 serum samples collected from patients in Mexico infected with dengue virus serotypes 1 and 2 (DENV-1 and DENV-2). Patients with dengue hemorrhagic fever (DHF) who were infected with DENV-1 showed higher levels of circulating NS1 than patients with dengue fever (DF) (P = 0.0175). Moreover, NS1 levels were higher in patients with primary infections with DENV-1 than in patient infected with DENV-2 (P < 0.0001) and in patients with primary infections with DENV-2 than in patients with secondary infections with DENV-2 (P = 0.0051). Unexpectedly, viremia levels were higher in patients with DF than in those with DHF infected with either DENV-1 or DENV-2 (P = 0.0019 and P = 0.001, respectively) and in patients with primary infections than those with secondary DENV-2 infections (P < 0.0001). Results indicate that levels of circulating NS1 vary according to the infecting serotype, immunologic status (primary or secondary infection), and dengue disease severity.
authors: Departament of Virology, Instituto de Diagnóstico y Referencia Epimiológicos, Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, Mexico. sidlch@yahoo.com.mx
authors: Sergio I
authors: SI
authors: de la Cruz-Hernández
authors: Hilario
authors: H
authors: Flores-Aguilar
authors: Silvia
authors: S
authors: González-Mateos
authors: Irma
authors: I
authors: López-Martinez
authors: Celia
authors: C
authors: Alpuche-Aranda
authors: Juan E
authors: JE
authors: Ludert
authors: Rosa M
authors: RM
authors: del Angel
mesh_terms: D003715:Dengue / Q000097:blood / Q000453:epidemiology* / Q000821:virology; D003716:Dengue Virus / Q000145:classification* / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D008800:Mexico / Q000453:epidemiology; D012367:RNA, Viral / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017361:Viral Nonstructural Proteins / Q000097:blood*; D014766:Viremia; D055815:Young Adult
chemical_list: C079340:NS1 protein, Dengue virus type 2; C082515:NS1 protein, dengue-1 virus; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins