title: Generation of a human bone marrow-derived mesenchymal stem cell line expressing and secreting high levels of bioactive α-melanocyte-stimulating hormone.
abstract: α-Melanocyte-stimulating hormone (α-MSH) functions as a mediator of inflammation and immunity; however, the short half-life and high dose needed limit the comprehensive clinical application of α-MSH. The aim of this study was to generate human bone marrow-derived mesenchymal stem cells (MSCs) that express and secrete high levels of bioactive α-MSH. MSCs were obtained from a normal donor and assessed for proliferation, surface markers, and adipogenic and osteogenic differentiation. A lentivirus-encoding α-MSH was constructed. MSCs were infected with this lentivirus-encoding α-MSH and assessed for stability and the expression and secretion of bioactive α-MSH. The cumulative MSC expansion rates pre- and post-lentivirus infection were not significantly different (P > 0.05). The MSCs remained stable after infection with the lentivirus-encoding α-MSH. The concentration of α-MSH in the supernatants of MSCs infected with the lentivirus-encoding α-MSH was 17.55 ng/ml (P < 0.001), and a melanin assay indicated that bioactive α-MSH was secreted from MSCs infected with the lentivirus-encoding α-MSH, with an optical absorbance at OD(405) of 0.886 (P < 0.001). These results suggested that MSCs were promising cell carriers for the expression and secretion of high levels of bioactive α-MSH.
authors: Department of Hepatobiliary Surgery, Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, China. zhongyuesi@gmail.com
authors: Yuesi
authors: Y
authors: Zhong
authors: Jianliang
authors: J
authors: Xu
authors: Meihai
authors: M
authors: Deng
authors: Bo
authors: B
authors: Liu
authors: Fucheng
authors: F
authors: Zhang
authors: Yuzhe
authors: Y
authors: Yuan
authors: Xuhui
authors: X
authors: Yang
authors: Ruiyun
authors: R
authors: Xu
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000235:genetics; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism / Q000821:virology; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D006207:Half-Life; D006801:Humans; D016086:Lentivirus / Q000235:genetics / Q000472:pathogenicity; D008543:Melanins / Q000096:biosynthesis; D008546:Melanoma, Experimental / Q000188:drug therapy; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D010012:Osteogenesis; D010446:Peptide Fragments / Q000235:genetics; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D000521:alpha-MSH / Q000235:genetics* / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D008543:Melanins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D000521:alpha-MSH; D000324:Adrenocorticotropic Hormone; C028238:ACTH (1-17)