title: Structural elucidation of a biologically active heteroglycan isolated from a hybrid mushroom of Pleurotus florida and Lentinula edodes.
abstract: A water soluble heteroglycan isolated from hot aqueous extract of fruit bodies of an edible hybrid mushroom Pfle1r of Pleurotus florida and Lentinula edodes consists of d-glucose, d-mannose, and d-galactose residues in a molar ratio of nearly 1:1:1. This polysaccharide showed macrophage, splenocyte, and thymocyte activation. On the basis of sugar hydrolysis, methylation, periodate oxidation study, and NMR studies (1H, 13C, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HSQC, and HMBC), the structure of the repeating unit of the polysaccharide was established as: -->6)-α-D-Galp-(1-->6)-α-D-Glcp-(1-->2<--1β-D-Manp.
authors: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors: Praloy K
authors: PK
authors: Maji
authors: Ipsita K
authors: IK
authors: Sen
authors: K Sanjana P
authors: KS
authors: Devi
authors: Tapas K
authors: TK
authors: Maiti
authors: Samir R
authors: SR
authors: Sikdar
authors: Syed S
authors: SS
authors: Islam
mesh_terms: D002240:Carbohydrate Sequence; D002678:Chimera; D009682:Magnetic Resonance Spectroscopy; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D020942:Shiitake Mushrooms / Q000737:chemistry*
chemical_list: D011134:Polysaccharides