title: Changes in structural characteristics of antioxidative soy protein hydrolysates resulting from scavenging of hydroxyl radicals.
abstract: Antioxidant activity of soy protein (SP) and its hydrolyzed peptides has been widely reported. During scavenging of radicals, these antioxidative compounds would be oxidatively modified, but their fate is not understood. The objective of this study was to evaluate the structural characteristics of SP hydrolysates (SPHs), compared to intact SP, when used to neutralize hydroxyl radicals (•OH). SPHs with degree of hydrolysis (DH) 1 to 5 were prepared with Alcalase. Antioxidant activity of SPHs was confirmed by lipid oxidation inhibition measured with thiobarbituric acid-reactive substances, ability to scavenge 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radicals, and ferrous ion chelation capability. Oxidation of SPHs was initiated by reaction with •OH generated from 0.1 mM FeCl(3) , 20 mM H(2) O(2) , and 1.0 mM ascorbate. After oxidative stress, carbonyl content of SPHs increased by 2- to 3-fold and sulfhydryl groups decreased by up to 42% compared to nonoxidized samples (P < 0.05). Methionine, histidine, and lysine residues were significantly reduced as a result of inactivating •OH (P < 0.05). Attenuated total reflectance-Fourier transform infrared and circular dichroism spectroscopy suggested the conversion of helical structure to strands and turns. Oxidatively modified SPHs had a lower intrinsic fluorescence intensity but similar solubility when compared to nonoxidized samples. These structural changes due to •OH stress may impact the ingredient interaction and functionality of SPHs in food products.
authors: Dept. of Animal and Food Sciences, Univ. of Kentucky, Lexington, KY 40546, USA.
authors: Jing
authors: J
authors: Zhao
authors: Youling L
authors: YL
authors: Xiong
authors: Dave H
authors: DH
authors: McNear
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D001205:Ascorbic Acid / Q000378:metabolism; D002614:Chelating Agents / Q000378:metabolism; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D016166:Free Radical Scavengers / Q000737:chemistry*; D006868:Hydrolysis; D017665:Hydroxyl Radical / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry; D010084:Oxidation-Reduction; D011492:Protein Hydrolysates / Q000737:chemistry*; D012995:Solubility; D030262:Soybean Proteins / Q000032:analysis / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013381:Subtilisins / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D000975:Antioxidants; D002614:Chelating Agents; D016166:Free Radical Scavengers; D008055:Lipids; D011492:Protein Hydrolysates; D030262:Soybean Proteins; D017392:Thiobarbituric Acid Reactive Substances; D017665:Hydroxyl Radical; D013381:Subtilisins; D001205:Ascorbic Acid