title: Wheat oxophytodienoate reductase gene TaOPR1 confers salinity tolerance via enhancement of abscisic acid signaling and reactive oxygen species scavenging.
abstract: The 12-oxo-phytodienoic acid reductases (OPRs) are classified into the two subgroups OPRI and OPRII. The latter proteins participate in jasmonic acid synthesis, while the function of the former ones is as yet unclear. We describe here the characterization of the OPRI gene TaOPR1, isolated from the salinity-tolerant bread wheat (Triticum aestivum) cultivar SR3. Salinity stress induced a higher level of TaOPR1 expression in the seedling roots of cv SR3 than in its parental cultivar, JN177. This induction was abolished when abscisic acid (ABA) synthesis was inhibited. The overexpression of TaOPR1 in wheat significantly enhanced the level of salinity tolerance, while its heterologous expression in Arabidopsis alleviated root growth restriction in the presence of salinity and oxidants and raised the sensitivity to ABA. In Arabidopsis, TaOPR1 promoted ABA synthesis and the ABA-dependent stress-responsive pathway, partially rescued the sensitivity of the Arabidopsis aba2 mutant defective in ABA synthesis to salinity, and improved the activities of reactive oxygen species scavengers and the transcription of their encoding genes while reducing malondialdehyde and reactive oxygen species levels. TaOPR1 did not interact with jasmonate synthesis or the jasmonate signaling pathway. Rather than serving purely as an antioxidant, we believe that TaOPR1 acts during episodes of abiotic stress response as a signaling compound associated with the regulation of the ABA-mediated signaling network.
authors: Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, Jinan, Shandong 250100, China.
authors: Wei
authors: W
authors: Dong
authors: Mengcheng
authors: M
authors: Wang
authors: Fei
authors: F
authors: Xu
authors: Taiyong
authors: T
authors: Quan
authors: Keqin
authors: K
authors: Peng
authors: Langtao
authors: L
authors: Xiao
authors: Guangmin
authors: G
authors: Xia
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism* / Q000494:pharmacology; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D003517:Cyclopentanes / Q000378:metabolism; D016166:Free Radical Scavengers / Q000378:metabolism*; D017343:Genes, Plant / Q000235:genetics; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008954:Models, Biological; D008969:Molecular Sequence Data; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D017382:Reactive Oxygen Species / Q000378:metabolism*; D055049:Salt Tolerance / Q000187:drug effects / Q000235:genetics*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics*; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects; D014908:Triticum / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000502:physiology
chemical_list: D003517:Cyclopentanes; D016166:Free Radical Scavengers; D054883:Oxylipins; D010940:Plant Proteins; D017382:Reactive Oxygen Species; C011006:jasmonic acid; D000040:Abscisic Acid; D006861:Hydrogen Peroxide; D044925:Oxidoreductases Acting on CH-CH Group Donors; C106360:12-oxophytodienoate reductase