title: Arabinose substitution degree in xylan positively affects lignocellulose enzymatic digestibility after various NaOH/H2SO4 pretreatments in Miscanthus.
abstract: Xylans are the major hemicelluloses in grasses, but their effects on biomass saccharification remain unclear. In this study, we examined the 79 representative Miscanthus accessions that displayed a diverse cell wall composition and varied biomass digestibility. Correlation analysis showed that hemicelluloses level has a strong positive effect on lignocellulose enzymatic digestion after NaOH or H(2)SO(4) pretreatment. Characterization of the monosaccharide compositions in the KOH-extractable and non-KOH-extractable hemicelluloses indicated that arabinose substitution degree of xylan is the key factor that positively affects biomass saccharification. The xylose/arabinose ratio after individual enzyme digestion revealed that the arabinose in xylan is partially associated with cellulose in the amorphous regions, which negatively affects cellulose crystallinity for high biomass digestibility. The results provide insights into the mechanism of lignocellulose enzymatic digestion upon pretreatment, and also suggest a goal for the genetic modification of hemicelluloses towards the bioenergy crop breeding of Miscanthus and grasses.
authors: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
authors: Fengcheng
authors: F
authors: Li
authors: Shuangfeng
authors: S
authors: Ren
authors: Wei
authors: W
authors: Zhang
authors: Zhengdan
authors: Z
authors: Xu
authors: Guosheng
authors: G
authors: Xie
authors: Yan
authors: Y
authors: Chen
authors: Yuanyuan
authors: Y
authors: Tu
authors: Qing
authors: Q
authors: Li
authors: Shiguang
authors: S
authors: Zhou
authors: Yu
authors: Y
authors: Li
authors: Fen
authors: F
authors: Tu
authors: Lin
authors: L
authors: Liu
authors: Yanting
authors: Y
authors: Wang
authors: Jianxiong
authors: J
authors: Jiang
authors: Jingping
authors: J
authors: Qin
authors: Shizhong
authors: S
authors: Li
authors: Qiwei
authors: Q
authors: Li
authors: Hai-Chun
authors: HC
authors: Jing
authors: Fasong
authors: F
authors: Zhou
authors: Neal
authors: N
authors: Gutterson
authors: Liangcai
authors: L
authors: Peng
mesh_terms: D001089:Arabinose / Q000378:metabolism*; D018533:Biomass; D002473:Cell Wall / Q000378:metabolism; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D006109:Poaceae / Q000378:metabolism*; D012972:Sodium Hydroxide; D013464:Sulfuric Acids; D014990:Xylans / Q000378:metabolism*
chemical_list: D013464:Sulfuric Acids; D014990:Xylans; C036909:lignocellulose; D012972:Sodium Hydroxide; D008031:Lignin; D001089:Arabinose; C033158:sulfuric acid