title: Effects of preoperative irradiation using fractioned electron beam on the healing process of colocolonic anastomosis in rats undergoing early and late surgical intervention.
abstract: OBJECTIVE
  To investigate the effects of preoperative fractioned irradiation using an electron beam on the healing process of colocolonic anastomoses in rats that underwent early and late surgical intervention.
  
  
  METHODS
  Thirty Wistar rats, distributed as follows: group A (surgery only), group B (fractionated irradiation for 30 days (if), surgery seven days after the end of it), group C (if for 30 days, and surgery after 30 days of termination). On the seventh postoperative day the anastomotic segment analysis was taken, using tension tests, histology and collagen deposition evaluation by computerized analysis.
  
  
  RESULTS
  Regarding the tension resistance of the anastomosis, there were no statistical differences (p=0.42). However, a significant increase in cells number in the inflammatory infiltrate in the group with a longer interval between surgery and pre op radiation (p<0.05). The collagen concentration had no significant variance.
  
  
  CONCLUSIONS
  The irradiation in divided doses increased local inflammatory cellularity when the surgery was performed later. This result did not affect the increase of complications, nor on the local concentration of collagen, achieving similar clinical outcomes.
authors: Postgraduate Program in Surgical Gastroenterology, UNIFESP, Sao Paulo, Brasil. jsimoes@uol.com.br
authors: Joaquim
authors: J
authors: Simões Neto
authors: José Alfredo dos
authors: JA
authors: Reis Neto
authors: Delcio
authors: D
authors: Matos
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D003094:Collagen / Q000032:analysis; D003106:Colon / Q000601:surgery*; D019583:Dose Fractionation, Radiation; D011300:Preoperative Care; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013997:Time Factors; D016896:Treatment Outcome; D014945:Wound Healing / Q000528:radiation effects*
chemical_list: D003094:Collagen