title: HDAC1-mSin3a-NCOR1, Dnmt3b-HDAC1-Egr1 and Dnmt1-PCNA-UHRF1-G9a regulate the NY-ESO1 gene expression.
abstract: The NY-ESO1 gene is a cancer/testis antigen considered to be suitable target for the immunotherapy of human malignancies. Despite the identification of the epigenetical silencing of the NY-ESO1 gene in a large variety of tumors, the molecular mechanism involved in this phenomenon is not fully elucidated. In two non epithelial cancers (glioma and mesothelioma), we found that the epigenetic regulation of the NY-ESO1 gene requires the sequential recruitment of the HDAC1-mSin3a-NCOR, Dnmt3b-HDAC1-Egr1 and Dnmt1-PCNA-UHRF1-G9a complexes. Thus, our data illustrate the orchestration of a sequential epigenetic mechanism including the histone deacetylation and methylation, and the DNA methylation processes.
authors: Centre de Recherche en Cancérologie Nantes-Angers, INSERM U892, Equipe Apoptose et Progression Tumorale, Equipe labellisée Ligue Nationale Contre le Cancer, 8 Quai Moncousu, BP 7021, 44007 Nantes, France. pierre-francois.cartron@univ-nantes.fr
authors: Pierre-François
authors: PF
authors: Cartron
authors: Christophe
authors: C
authors: Blanquart
authors: Eric
authors: E
authors: Hervouet
authors: Marc
authors: M
authors: Gregoire
authors: François M
authors: FM
authors: Vallette
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics*; D022762:CCAAT-Enhancer-Binding Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000378:metabolism; D019175:DNA Methylation; D051766:Early Growth Response Protein 1 / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D005910:Glioma / Q000235:genetics* / Q000378:metabolism; D006649:Histocompatibility Antigens / Q000378:metabolism; D056284:Histone Deacetylase 1 / Q000378:metabolism; D011495:Histone-Lysine N-Methyltransferase / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D008654:Mesothelioma / Q000235:genetics* / Q000378:metabolism; D046912:Multiprotein Complexes / Q000378:metabolism; D056971:Nuclear Receptor Co-Repressor 1 / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D056264:Sin3 Histone Deacetylase and Corepressor Complex; D044767:Ubiquitin-Protein Ligases; D000097584:DNA Methyltransferase 3B
chemical_list: D000951:Antigens, Neoplasm; D022762:CCAAT-Enhancer-Binding Proteins; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; D051766:Early Growth Response Protein 1; D006649:Histocompatibility Antigens; D056284:Histone Deacetylase 1; D011495:Histone-Lysine N-Methyltransferase; D008565:Membrane Proteins; D046912:Multiprotein Complexes; D056971:Nuclear Receptor Co-Repressor 1; D018809:Proliferating Cell Nuclear Antigen; D012097:Repressor Proteins; D056264:Sin3 Histone Deacetylase and Corepressor Complex; D044767:Ubiquitin-Protein Ligases; D000097584:DNA Methyltransferase 3B; C104948:CTAG1B protein, human; C539127:NCOR1 protein, human; C099683:SIN3A transcription factor; C000619380:DNMT1 protein, human; C445405:EHMT2 protein, human; C406364:UHRF1 protein, human; C110550:HDAC1 protein, human