title: Dietary n-3 long-chain polyunsaturated fatty acids modify phosphoenolpyruvate carboxykinase activity and lipid synthesis from glucose in adipose tissue of rats fed a high-sucrose diet.
abstract: Long-chain polyunsaturated n-3 fatty acids (n-3 LCPUFAs) have hypolipidemic effects and modulate intermediary metabolism to prevent or reverse insulin resistance in a way that is not completely elucidated. Here, effects of these fatty acids on the lipid profile, phosphoenolpyruvate carboxykinase (PEPCK) activity, lipid synthesis from glucose in epididymal adipose tissue (Ep-AT) and liver were investigated. Male rats were fed a high-sucrose diet (SU diet), containing either sunflower oil or a mixture of sunflower and fish oil (SU-FO diet), and the control group was fed a standard diet. After 13 weeks, liver, adipose tissue and blood were harvested and analysed. The dietary n-3 LCPUFAs prevented sucrose-induced increase in adiposity and serum free fat acids, serum and hepatic triacylglycerol and insulin levels. Furthermore, these n-3 LCPUFAs decreased lipid synthesis from glucose and increased PEPCK activity in the Ep-AT of rats fed the SU-FO diet compared to those fed the SU diet, besides reducing lipid synthesis from glucose in hepatic tissue. Thus, the inclusion of n-3 LCPUFAs in the diet may be beneficial for the prevention or attenuation of dyslipidemia and insulin resistance, and for reducing the risk of related chronic diseases.
authors: Biochemistry Postgraduate Program, Institute of Biologic Sciences of Health, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors: Lisiane G
authors: LG
authors: Londero
authors: Débora K
authors: DK
authors: Rieger
authors: Fernanda
authors: F
authors: Hansen
authors: Simone L
authors: SL
authors: Silveira
authors: Tiago L
authors: TL
authors: Martins
authors: Francisco
authors: F
authors: Lulhier
authors: Roselis S
authors: RS
authors: da Silva
authors: Diogo O
authors: DO
authors: Souza
authors: Marcos L S
authors: ML
authors: Perry
authors: Adriano M
authors: AM
authors: de Assis
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D019422:Dietary Sucrose / Q000008:administration & dosage* / Q000494:pharmacology; D019587:Dietary Supplements; D004789:Enzyme Activation / Q000187:drug effects; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage* / Q000494:pharmacology*; D005947:Glucose / Q000737:chemistry / Q000378:metabolism*; D008055:Lipids / Q000096:biosynthesis*; D008297:Male; D019764:Phosphoenolpyruvate Carboxykinase (ATP) / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D019422:Dietary Sucrose; D015525:Fatty Acids, Omega-3; D008055:Lipids; D019764:Phosphoenolpyruvate Carboxykinase (ATP); D005947:Glucose
keywords: adipose tissue; high-sucrose diet; insulin resistance; n-3 long-chain polyunsaturated fatty acids; phosphoenolpyruvate carboxykinase