title: Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal.
abstract: Chlamydomonas reinhardtii is widely used to study many biological processes including biofuel production. Here, we present a rapid transformation technique for cell-walled Chlamydomonas strains without cell-wall removal using a square electric pulses-generating electroporator. This method could be applied to transformation of other industrially useful algae by optimizing the electric conditions.
authors: Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan.
authors: Takashi
authors: T
authors: Yamano
authors: Hiro
authors: H
authors: Iguchi
authors: Hideya
authors: H
authors: Fukuzawa
mesh_terms: D002473:Cell Wall; D016825:Chlamydomonas reinhardtii / Q000235:genetics*; D018274:Electroporation / Q000379:methods*; D014170:Transformation, Genetic