title: Comparative study and histomorphometric analysis of bone allografts lyophilized and sterilized by autoclaving, gamma irradiation and ethylene oxide in rats.
abstract: OBJECTIVE
  To compare three sterilization methods (autoclave, gamma irradiation and ethylene oxide) over non demineralized lyophilized bone allografts.
  
  
  METHODS
  Bone allografts were implanted on paravertebral muscles of 21 rats. After 30 days animals were sacrificed and grafts underwent comparative analysis regarding histomorphometric and macroscopic parameters.
  
  
  RESULTS
  Allografts that underwent the three sterilization methods presents similar weight gain, cortical thickness similar to control group, and less fibrosis than the control group. Grafts that underwent sterilization in autoclave presented less presence of multinucleated giant cells, although not statistically significant. There was also no statistically significant difference regarding mineralization on the three groups.
  
  
  CONCLUSIONS
  The three sterilization methods cause similar effects on bone allografts regarding macroscopic and histomorphometric parameters.
authors: Antonio Prudente Foundation, Sao Paulo, Brazil. omadr@terra.com.br
authors: Otavio Machado de
authors: OM
authors: Almeida
authors: Wanda
authors: W
authors: Jorgetti
authors: Denis
authors: D
authors: Oksman
authors: Camilo
authors: C
authors: Jorgetti
authors: Diógenes Laércio
authors: DL
authors: Rocha
authors: Rolf
authors: R
authors: Gemperli
mesh_terms: D000818:Animals; D016025:Bone Transplantation / Q000295:instrumentation / Q000379:methods*; D005027:Ethylene Oxide; D005355:Fibrosis / Q000473:pathology; D005612:Freeze Drying / Q000379:methods; D005720:Gamma Rays; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D015203:Reproducibility of Results; D013242:Sterilization / Q000295:instrumentation / Q000379:methods*; D013977:Tibia / Q000737:chemistry* / Q000473:pathology / Q000528:radiation effects; D014184:Transplantation, Homologous / Q000379:methods
chemical_list: D005027:Ethylene Oxide