title: Recycling of peptidyl-tRNAs by peptidyl-tRNA hydrolase counteracts azithromycin-mediated effects on Pseudomonas aeruginosa.
abstract: Acute and chronic infections caused by the opportunistic pathogen Pseudomonas aeruginosa pose a serious threat to human health worldwide, and its increasing resistance to antibiotics requires alternative treatments that are more effective than available strategies. Clinical studies have clearly demonstrated that cystic fibrosis (CF) patients with chronic P. aeruginosa infections benefit from long-term low-dose azithromycin (AZM) treatment. Immunomodulating activity, the impact of AZM on the expression of quorum-sensing-dependent virulence factors, type three secretion, and motility in P. aeruginosa seem to contribute to the therapeutic response. However, to date, the molecular mechanisms underlying these AZM effects have remained elusive. Our data indicate that the AZM-mediated phenotype is caused by a depletion of the intracellular pools of tRNAs available for protein synthesis. Overexpression of the P. aeruginosa peptidyl-tRNA hydrolase, which recycles the tRNA from peptidyl-tRNA drop-off during translation, counteracted the effects of AZM on stationary-phase cell killing, cytotoxicity, and the production of rhamnolipids and partially restored swarming motility. Intriguingly, the exchange of a rare for a frequent codon in rhlR also explicitly diminished the AZM-mediated decreased production of rhamnolipids. These results indicate that depletion of the tRNA pools by AZM seems to affect the translation of genes that use rare aminoacyl-tRNA isoacceptors to a great extent and might explain the selective activity of AZM on the P. aeruginosa proteome and possibly also on the protein expression profiles of other bacterial pathogens.
authors: Department of Molecular Bacteriology, Helmholtz Center for Infection Research, Braunschweig, Germany.
authors: Julia
authors: J
authors: Gödeke
authors: Christian
authors: C
authors: Pustelny
authors: Susanne
authors: S
authors: Häussler
mesh_terms: D017963:Azithromycin / Q000494:pharmacology*; D018407:Bacterial Physiological Phenomena / Q000187:drug effects; D001426:Bacterial Proteins / Q000378:metabolism*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D012346:RNA, Transfer, Amino Acyl; C019037:tRNA, peptidyl-; D017963:Azithromycin; D002265:Carboxylic Ester Hydrolases; C019674:aminoacyl-tRNA hydrolase