title: Differential proteomic profiles from distinct Toxoplasma gondii strains revealed by 2D-difference gel electrophoresis.
abstract: Toxoplasma gondii is an obligate intracellular protozoan that infects mammals and birds. Human infection during pregnancy may cause severe damage to the fetus. Reactivation of latent infection in immunocompromised patients can cause life-threatening encephalitis. T. gondii strains are highly diverse but only a few lineages (Type I, II and III) are widely spread. In mouse model, Type I strains are highly virulent, whereas Type II and III strains are intermediately or non virulent. It is not clear how much quantitative difference exists in proteomic profiles among these distinct T. gondii lineages. In the present study, the proteomic profiles of T. gondii tachyzoites from these lineages were investigated by two dimensional fluorescence difference gel electrophoresis (2D-DIGE) and mass spectrometry (MS) technologies. A total of 2321 protein spots were detected. Overall, the GT1 strain of Type I lineage and the strain PTG of Type II lineage have highly similar proteomic profiles and both are different from that of the CTG strain of Type III lineage. Eighty-four protein spots were differentially expressed by greater than 1.5-fold in relative abundance and 10 of them were identified to 7 T. gondii proteins in existing database. Investigation of the quantitative differences in proteomics among distinct T. gondii strains should facilitate our understanding of difference in biological processes and pathogenesis of distinct T. gondii genotypes, which will provide basic information to determine treatment regimen for different manifestation of toxoplasmosis.
authors: Department of Parasitology, Shandong University School of Medicine, Jinan, Shandong, China; Department of Microbiology, The University of Tennessee, Knoxville, TN 37996-0845, USA. zhouhy@sdu.edu.cn
authors: Huaiyu
authors: H
authors: Zhou
authors: Qunli
authors: Q
authors: Zhao
authors: Lachhman
authors: L
authors: Das Singla
authors: Juan
authors: J
authors: Min
authors: Shenyi
authors: S
authors: He
authors: Hua
authors: H
authors: Cong
authors: Ying
authors: Y
authors: Li
authors: Chunlei
authors: C
authors: Su
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D005347:Fibroblasts / Q000469:parasitology; D052816:Foreskin / Q000166:cytology; D006801:Humans; D021621:Imaging, Three-Dimensional; D008297:Male; D020543:Proteome / Q000737:chemistry*; D040901:Proteomics / Q000379:methods; D015800:Protozoan Proteins / Q000032:analysis* / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014122:Toxoplasma / Q000737:chemistry* / Q000145:classification
chemical_list: D020543:Proteome; D015800:Protozoan Proteins