title: Layer-specific excitatory circuits differentially control recurrent network dynamics in the neocortex.
abstract: In the absence of external stimuli, the mammalian neocortex shows intrinsic network oscillations. These dynamics are characterized by translaminar assemblies of neurons whose activity synchronizes rhythmically in space and time. How different cortical layers influence the formation of these spontaneous cellular assemblies is poorly understood. We found that excitatory neurons in supragranular and infragranular layers have distinct roles in the regulation of intrinsic low-frequency oscillations in mice in vivo. Optogenetic activation of infragranular neurons generated network activity that resembled spontaneous events, whereas photoinhibition of these same neurons substantially attenuated slow ongoing dynamics. In contrast, light activation and inhibition of supragranular cells had modest effects on spontaneous slow activity. This study represents, to the best of our knowledge, the first causal demonstration that excitatory circuits located in distinct cortical layers differentially control spontaneous low-frequency dynamics.
authors: Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Genova, Italy.
authors: Riccardo
authors: R
authors: Beltramo
authors: Giulia
authors: G
authors: D'Urso
authors: Marco
authors: M
authors: Dal Maschio
authors: Pasqualina
authors: P
authors: Farisello
authors: Serena
authors: S
authors: Bovetti
authors: Yoanne
authors: Y
authors: Clovis
authors: Glenda
authors: G
authors: Lassi
authors: Valter
authors: V
authors: Tucci
authors: Davide
authors: D
authors: De Pietri Tonelli
authors: Tommaso
authors: T
authors: Fellin
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D001426:Bacterial Proteins / Q000235:genetics; D000075402:Channelrhodopsins; D004558:Electric Stimulation; D004569:Electroencephalography; D018274:Electroporation; D005260:Female; D066298:In Vitro Techniques; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008959:Models, Neurological; D019579:Neocortex / Q000166:cytology* / Q000502:physiology*; D009415:Nerve Net / Q000502:physiology*; D009434:Neural Pathways / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D017711:Nonlinear Dynamics; D018408:Patch-Clamp Techniques; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D010775:Photic Stimulation; D011247:Pregnancy; D011506:Proteins / Q000235:genetics; D022661:RNA, Untranslated; D054839:Retinol-Binding Proteins, Plasma / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D000075402:Channelrhodopsins; C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D008164:Luminescent Proteins; D011506:Proteins; D022661:RNA, Untranslated; C502487:Rbp4 protein, mouse; D054839:Retinol-Binding Proteins, Plasma; C054562:yellow fluorescent protein, Bacteria; D010751:Phosphopyruvate Hydratase