title: Antiproliferative activity of daidzein and genistein may be related             to ERα/c-erbB-2 expression in human breast cancer cells.
abstract: In this study, we investigated the antiproliferative activity of the isoflavones             daidzein and genistein in three breast cancer cell lines with different patterns             of estrogen receptor (ER) and c‑erbB‑2 protein expression (ERα‑positive MCF‑7             cells, c‑erbB‑2‑positive SK‑BR‑3 cells and ERα/c‑erbB‑2‑positive ZR‑75‑1). After             treatment at various concentrations (1‑200 µM for 72 h), the effect of daidzein             and genistein on the proliferation of different cell types varied; these effects             were found to be associated with ERα and c‑erbB‑2 expression. Daidzein and genistein             exhibited biphasic effects (stimulatory or inhibitory) on proliferation and ERα             expression in MCF‑7 cells. Although 1 µM daidzein significantly stimulated cell             growth, ERα expression was unaffected. However, genistein showed marked increases             in proliferation and ERα expression after exposure to <10 µM genistein. Notably,             the inhibition of cell proliferation by 200 µM genistein was greater compared             to that by daidzein at the same concentration. Daidzein and genistein significantly             inhibited proliferation of SK‑BR‑3 and ZR‑75‑1 cells in a dose‑dependent manner.             In addition, ERα and c‑erbB‑2 expression was reduced by daidzein and genistein             in both SK‑BR‑3 and ZR‑75‑1 cells in a dose‑dependent manner. However, the effect             of genistein was greater compared to that of daidzein. In conclusion, the isoflavones             daidzein and genistein showed anti‑breast cancer activity, which was associated             with expression of the ERα and c‑erbB‑2 receptors.
authors: Plant Resources Research Institute, Duksung Women's University, Seoul 132-714, Republic of Korea. ejchoi@duksung.ac.kr
authors: Eun Jeong
authors: EJ
authors: Choi
authors: Gun-Hee
authors: GH
authors: Kim
mesh_terms: D000970:Antineoplastic Agents / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D005260:Female; D019833:Genistein / Q000633:toxicity*; D006801:Humans; D007529:Isoflavones / Q000633:toxicity*; D061986:MCF-7 Cells; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D047628:Estrogen Receptor alpha; D019833:Genistein; D007529:Isoflavones; D018719:Erb-b2 Receptor Tyrosine Kinases; C004742:daidzein