title: Selection and characterization of colorectal cancer cell-specific peptides.
abstract: To develop a novel peptide probe for imaging detection of colorectal cancer, a 12-mer phage display library was used to select peptides that bind specifically to the human colorectal cancer cell line Caco-2. After four rounds of panning, four phage clones that bound specifically to the Caco-2 cells were selected. The phage clone SP-2 had a particularly high affinity and specificity for Caco-2 cells. This clone was identified using a series of methods. The peptide SP-2 that was displayed on phage SP-2 exhibited a high specificity to Caco-2 cells. Thus, the peptide SP-2 could be a candidate probe for the detection of colorectal cancer.
authors: Department of Cell Biology, College of Life Sciences, Shaanxi Normal University, 199 South Chang'an Road, Xi'an 710062, People's Republic of China.
authors: Han
authors: H
authors: Wang
authors: Caixia
authors: C
authors: Ma
authors: Rui
authors: R
authors: Li
authors: Yonge
authors: Y
authors: Guo
authors: Yakun
authors: Y
authors: He
authors: Xiaojing
authors: X
authors: Wang
authors: Yiwen
authors: Y
authors: Chen
authors: Yingchun
authors: Y
authors: Hou
mesh_terms: D001667:Binding, Competitive; D018938:Caco-2 Cells; D015179:Colorectal Neoplasms / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D009363:Neoplasm Proteins / Q000737:chemistry*; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D009363:Neoplasm Proteins; D019151:Peptide Library; D010455:Peptides