title: Intracerebral neural stem cell transplantation improved the auditory of mice with presbycusis.
abstract: Stem cell-based regenerative therapy is a potential cellular therapeutic strategy for patients with incurable brain diseases. Embryonic neural stem cells (NSCs) represent an attractive cell source in regenerative medicine strategies in the treatment of diseased brains. Here, we assess the capability of intracerebral embryonic NSCs transplantation for C57BL/6J mice with presbycusis in vivo. Morphology analyses revealed that the neuronal rate of apoptosis was lower in the aged group (10 months of age) but not in the young group (2 months of age) after NSCs transplantation, while the electrophysiological data suggest that the Auditory Brain Stem Response (ABR) threshold was significantly decreased in the aged group at 2 weeks and 3 weeks after transplantation. By contrast, there was no difference in the aged group at 4 weeks post-transplantation or in the young group at any time post-transplantation. Furthermore, immunofluorescence experiments showed that NSCs differentiated into neurons that engrafted and migrated to the brain, even to sites of lesions. Together, our results demonstrate that NSCs transplantation improve the auditory of C57BL/6J mice with presbycusis.
authors: Department of Otolaryngology Head and Neck Surgery, Institute of Surgery Research & Daping Hospital, Third Military Medical University Chongqing 400042, China.
authors: Hongmiao
authors: H
authors: Ren
authors: Jichuan
authors: J
authors: Chen
authors: Yinan
authors: Y
authors: Wang
authors: Shichang
authors: S
authors: Zhang
authors: Bo
authors: B
authors: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001303:Auditory Cortex / Q000473:pathology / Q000502:physiology*; D001309:Auditory Threshold / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D004195:Disease Models, Animal; D053595:Embryonic Stem Cells / Q000637:transplantation / Q000648:ultrastructure; D016057:Evoked Potentials, Auditory, Brain Stem / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D058953:Neural Stem Cells / Q000637:transplantation* / Q000648:ultrastructure; D011304:Presbycusis / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D012038:Regeneration; D033581:Stem Cell Transplantation / Q000379:methods*; D016896:Treatment Outcome
keywords: C57BL/6J mice; Presbycusis; apoptosis; auditory brain stem response (ABR); neural stem cells; primary auditory cortex; transplantation