title: Adhesion, vitality and osteogenic differentiation capacity of adipose derived stem cells seeded on nitinol nanoparticle coatings.
abstract: Autologous cells can be used for a bioactivation of osteoimplants to enhance osseointegration. In this regard, adipose derived stem cells (ASCs) offer interesting perspectives in implantology because they are fast and easy to isolate. However, not all materials licensed for bone implants are equally suited for cell adhesion. Surface modifications are under investigation to promote cytocompatibility and cell growth. The presented study focused on influences of a Nitinol-nanoparticle coating on ASCs. Possible toxic effects as well as influences on the osteogenic differentiation potential of ASCs were evaluated by viability assays, scanning electron microscopy, immunofluorescence and alizarin red staining. It was previously shown that Nitinol-nanoparticles exert no cell toxic effects to ASCs either in soluble form or as surface coating. Here we could demonstrate that a Nitinol-nanoparticle surface coating enhances cell adherence and growth on Nitinol-surfaces. No negative influence on the osteogenic differentiation was observed. Nitinol-nanoparticle coatings offer new possibilities in implantology research regarding bioactivation by autologous ASCs, respectively enhancement of surface attraction to cells.
authors: Department for Plastic- Hand- and Reconstructive Surgery, Hannover Medical School, Hannover, Germany. strauss.sarah@mh-hannover.de
authors: Sarah
authors: S
authors: Strauss
authors: Anne
authors: A
authors: Neumeister
authors: Stephan
authors: S
authors: Barcikowski
authors: Dietmar
authors: D
authors: Kracht
authors: Jörn W
authors: JW
authors: Kuhbier
authors: Christine
authors: C
authors: Radtke
authors: Kerstin
authors: K
authors: Reimers
authors: Peter M
authors: PM
authors: Vogt
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000497:Alloys / Q000009:adverse effects* / Q000737:chemistry; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000009:adverse effects* / Q000737:chemistry; D006801:Humans; D051379:Mice; D053758:Nanoparticles / Q000009:adverse effects* / Q000737:chemistry; D010012:Osteogenesis / Q000187:drug effects; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D054457:Tissue Scaffolds / Q000009:adverse effects / Q000737:chemistry
chemical_list: D000497:Alloys; D020099:Coated Materials, Biocompatible; C013616:nitinol