title: A second combinatorial immune receptor in monocytes/macrophages is based on the TCRγδ.
abstract: Recent evidence indicates that monocytes and macrophages express T cell receptor (TCR)αβ-like combinatorial immune receptors. Here, we demonstrate the presence of a second recombinatorial immunoreceptor, which is structurally based on the TCR γ- and δ-chains, in human and murine monocytes and differentially activated macrophages (referred to here as TCRL(m)γδ). In vitro, infection of macrophages with mycobacteria and gram positive or gram negative bacteria induced expression of donor-specific and differential TCRL(m)Vδ repertoires indicating that the novel immunoreceptor represents a dynamic flexible host defense system that responds to bacterial challenge. In vivo, we find that TCRL(m)γδ bearing macrophages, which express highly restricted repertoires of the antigen-binding Vδ chain, accumulate in the cerebrospinal fluid in acute bacterial meningitis and in advanced lesions of atherosclerosis. These results identify an as yet unrecognized monocyte/macrophage subpopulation that bears combinatorial TCRL(m)γδ immune receptors, and is associated with both acute and chronic inflammatory diseases. Moreover, they indicate that the monocytic lineage uses the same bipartite system of TCRαβ/TCRγδ-based combinatorial immune receptors that is present in T cells. Our findings suggest specific roles of monocytes/macrophages in various inflammatory conditions and lend further evidence that flexible immune recognition in higher vertebrates operates on a broader cellular basis than previously thought.
authors: Institute for Clinical Chemistry, University of Heidelberg, Medical Faculty Mannheim, D-68167 Mannheim, Germany.
authors: Tina
authors: T
authors: Fuchs
authors: Kerstin
authors: K
authors: Puellmann
authors: Martin
authors: M
authors: Hahn
authors: Claudia
authors: C
authors: Dollt
authors: Ioanna
authors: I
authors: Pechlivanidou
authors: Ilja
authors: I
authors: Ovsiy
authors: Julia
authors: J
authors: Kzhyshkowska
authors: Alexei
authors: A
authors: Gratchev
authors: Julian
authors: J
authors: Fleig
authors: Alexander
authors: A
authors: Emmert
authors: Michael
authors: M
authors: Neumaier
authors: Alexander W
authors: AW
authors: Beham
authors: Wolfgang E
authors: WE
authors: Kaminski
mesh_terms: D000208:Acute Disease; D056704:Adaptive Immunity; D000368:Aged; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D050197:Atherosclerosis / Q000276:immunology / Q000473:pathology; D001424:Bacterial Infections / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D004926:Escherichia coli / Q000276:immunology; D015870:Gene Expression / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D008264:Macrophages / Q000166:cytology / Q000276:immunology* / Q000382:microbiology; D008297:Male; D016920:Meningitis, Bacterial / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D009000:Monocytes / Q000166:cytology / Q000276:immunology* / Q000382:microbiology; D009163:Mycobacterium bovis / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics / Q000276:immunology*; D020395:Receptors, Interleukin-7 / Q000172:deficiency / Q000235:genetics / Q000276:immunology; D013211:Staphylococcus aureus / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000382:microbiology
chemical_list: D000942:Antigens, Bacterial; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D020395:Receptors, Interleukin-7