title: Involvement of early growth response-2 (Egr-2) in lipopolysaccharide-induced neuroinflammation.
abstract: Early growth response-2 (Egr-2) protein is a transcription factor, which belongs to Egr family which involve in modulating the peripheral immune response, by means of the induction of differentiation of lymphocyte precursors, activation of T and B cells. Egr-2 plays essential roles in peripheral nerve myelination, adipogenesis, tissue repair and fibrosis, immune tolerance; however, its regulation and role in central nervous system (CNS) remain poorly understood. In contrast to Egr-1, which has been extensively investigated, the regulation and function of Egr-2 remains less well characterized. To elaborate whether Egr-2 was involved in CNS injury, we performed a neuroinflammatory model by lipopolysaccharide (LPS) lateral ventral injection in adult rats. Egr-2 expression was strongly induced in active glia cells (astrocytes and microglias) in inflamed brain cortex. In vitro studies indicated that the upregulation of Egr-2 may be involved in the subsequent glia cellular activation following LPS exposure; and knock down of Egr-2 in primary mixed glial cultures (MGC) by siRNA showed that Egr-2 promoted the synthesis of TNF-α. Collectively, these results suggested Egr-2 may be important in host defense in CNS immune response, which might provide a potential target to the treatment of neuroinflammation.
authors: Department of Neurology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province 226001, People's Republic of China.
authors: Yaohua
authors: Y
authors: Yan
authors: Xiang
authors: X
authors: Tan
authors: Xinmin
authors: X
authors: Wu
authors: Bai
authors: B
authors: Shao
authors: Xiaohong
authors: X
authors: Wu
authors: Jianhua
authors: J
authors: Cao
authors: Jian
authors: J
authors: Xu
authors: Wei
authors: W
authors: Jin
authors: Lei
authors: L
authors: Li
authors: Wei
authors: W
authors: Xu
authors: Xijun
authors: X
authors: Wang
authors: Yilu
authors: Y
authors: Gao
authors: Gang
authors: G
authors: Cui
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D002493:Central Nervous System Diseases / Q000235:genetics* / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D051767:Early Growth Response Protein 2 / Q000235:genetics* / Q000378:metabolism*; D007249:Inflammation / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism*; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D017628:Microglia / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D051767:Early Growth Response Protein 2; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha