title: Fel d 1-airway inflammation prevention and treatment by co-immunization vaccine via induction of CD4+CD25-Foxp3+ Treg cells.
abstract: Pet allergens are major causes for asthma and allergic rhinitis. Fel d 1 protein, a key pet allergen from domestic cat, can sensitize host and trigger asthma attack. In this study, we report that co-immunization with recombinant Fel d 1 protein (rFel d 1) plus plasmid DNA that contains Fe1 d 1 gene was effective in preventing and treating the natural Fel d 1 (nFel d 1) induced allergic airway inflammation in mice. A population of T regulatory cells (iTreg) exhibiting a CD4+CD25-Foxp3+ phenotype and expressing IL-10 and TGF-β was induced by this co-immunization strategy. Furthermore, after adoptive transfers of the iTreg cells, mice that were pre-sensitized and challenged with nFel d 1 exhibited less signs of allergic inflammation, AHR and a reduced allergic immune response. These data indicate that co-immunization with DNA and protein mixture vaccine may be an effective treatment for cat allergy.
authors: State Key Laboratory for Agro-Biotechnology; China Agricultural University; Beijing, P.R. China.
authors: Yechun
authors: Y
authors: Pei
authors: Shuang
authors: S
authors: Geng
authors: Lin
authors: L
authors: Liu
authors: Fengxiang
authors: F
authors: Yan
authors: Hong
authors: H
authors: Guan
authors: Jian
authors: J
authors: Hou
authors: Yongfu
authors: Y
authors: Chen
authors: Bin
authors: B
authors: Wang
authors: Xiaorong
authors: X
authors: An
mesh_terms: D000818:Animals; D001249:Asthma / Q000517:prevention & control / Q000628:therapy*; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry / Q000276:immunology; D002415:Cats; D004195:Disease Models, Animal; D005260:Female; D051858:Forkhead Transcription Factors / Q000032:analysis*; D006023:Glycoproteins / Q000008:administration & dosage / Q000276:immunology*; D053645:Interleukin-2 Receptor alpha Subunit / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D012220:Rhinitis / Q000517:prevention & control / Q000628:therapy*; D050378:T-Lymphocytes, Regulatory / Q000737:chemistry / Q000276:immunology*; D016896:Treatment Outcome; D014611:Vaccination / Q000379:methods; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D006023:Glycoproteins; C508018:Il2ra protein, mouse; D053645:Interleukin-2 Receptor alpha Subunit; D019444:Vaccines, DNA; C059296:Fel d 1 protein, Felis domesticus
keywords: Fel d 1; T regulatory cell; airway inflammation; co-immunization; vaccine