title: Peptides binding to the hunger hormone ghrelin.
abstract: The neutralisation of circulating ghrelin, the only known peripheral orexigenic peptide hormone, is a promising approach for the pharmacological treatment of obesity. To select peptides with an affinity towards ghrelin, 4 selection procedures were carried out with random peptide phage display libraries Ph.D.-7 and Ph.D.-12. Due to the absence of a common consensus motif, a stepwise elimination approach was used. The pool of selected peptides displaying phage clones was thoroughly examined to remove any potential target-unrelated peptides. The affinity of the remaining phage clones for ghrelin was tested with ELISA. An analysis of the binding properties revealed four-phage displayed peptides that bind to ghrelin with moderate affinity, with ADTVPRH and MEMKKTHPVLGA being the most specific. Additional advantage of peptide MEMKKTHPVLGA is an indication of binding to octanoyl group on N-terminal part of ghrelin, involved in receptor interaction. Hence peptide MEMKKTHPVLGA represents the most suitable lead for further investigation.
authors: Department of Pharmaceutical Biology, Faculty of Pharmacy, Ljubljana, Slovenia. miha.vodnik@ff a.uni-lj.si
authors: M
authors: M
authors: Vodnik
authors: P
authors: P
authors: Molek
authors: B
authors: B
authors: Strukelj
authors: M
authors: M
authors: Lunder
mesh_terms: D000595:Amino Acid Sequence; D002999:Clone Cells; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D054439:Ghrelin / Q000378:metabolism*; D006801:Humans; D006815:Hunger; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D054439:Ghrelin; D019151:Peptide Library; D010455:Peptides