title: Quantitative heterogeneous immunoassays in protein modified polydimethylsiloxane microfluidic channels for rapid detection of disease biomarkers.
abstract: Conventional detection of disease biomarkers employs techniques such as lateral-flow assays or central laboratory-based enzyme-linked immunosorbent assays (ELISA). Miniaturization and performance improvement of such traditional immunoassays using microfluidic technologies has proved promising in producing rapid, sensitive and automated next-generation immunosensors for quantitative diagnoses in the point-of-care setting. In this article a poly(dimethylsiloxane) (PDMS)-based immunosensor is presented for rapid detection of C-reactive protein. PDMS is selected in part because of the vast popularity of using PDMS as a material for microfluidic devices and in part because of the challenge of obtaining a stable surface coating with PDMS for immunosensing applications. Practical procedures for fabrication, surface modification, and preservation of the microfluidic immuno-chips as well as detailed descriptions of performing the microfluidic heterogeneous assay are presented.
authors: Department of Mechanical, Industrial and Systems Engineering, University of Rhode Island, Kingston, RI, USA. lip@egr.uri.edu
authors: Peng
authors: P
authors: Li
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation*; D002097:C-Reactive Protein / Q000032:analysis* / Q000378:metabolism; D002417:Cattle; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004194:Disease; D005976:Glutaral / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D013205:Staphylococcal Protein A / Q000737:chemistry / Q000378:metabolism; D013696:Temperature
chemical_list: D015415:Biomarkers; D004129:Dimethylpolysiloxanes; D013205:Staphylococcal Protein A; D012710:Serum Albumin, Bovine; C013830:baysilon; D002097:C-Reactive Protein; D005976:Glutaral