title: Evaluation of Epstein-Barr virus, human herpesvirus 6 (HHV-6), and HHV-8 antiviral drug susceptibilities by use of real-time-PCR-based assays.
abstract: Evaluation of candidate antiviral drugs against Epstein-Barr virus (EBV), human herpesvirus 6 (HHV-6), and HHV-8 is hampered by the lack of convenient laboratory assays. We developed real-time quantitative PCR assays performed on supernatants of lymphoma cell lines and determined the 50% inhibitory concentrations (IC50s) of nucleoside, nucleotide, and pyrophosphate analogues against these herpesviruses.
authors: Infectious Disease Research Centre, Laval University, Quebec City, Quebec, Canada.
authors: Lotfi
authors: L
authors: Bounaadja
authors: Jocelyne
authors: J
authors: Piret
authors: Nathalie
authors: N
authors: Goyette
authors: Guy
authors: G
authors: Boivin
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004854:Herpesvirus 4, Human / Q000187:drug effects*; D015654:Herpesvirus 6, Human / Q000187:drug effects*; D019288:Herpesvirus 8, Human / Q000187:drug effects*; D006801:Humans; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests / Q000379:methods; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D000998:Antiviral Agents