title: PCR-based detection of resistance genes in anaerobic bacteria isolated from intra-abdominal infections.
abstract: Little information is available on the distribution of antimicrobial resistance genes in anaerobes in Japan. To understand the background of antimicrobial resistance in anaerobes involved in intra-abdominal infections, we investigated the distribution of eight antimicrobial resistance genes (cepA, cfiA, cfxA, ermF, ermB, mefA, tetQ, and nim) and a mutation in the gyrA gene in a total of 152 organisms (Bacteroides spp., Prevotella spp., Fusobacterium spp., Porphyromonas spp., Bilophila wadsworthia, Desulfovibrio desulfuricans, Veillonella spp., gram-positive cocci, and non-spore-forming gram-positive bacilli) isolated between 2003 and 2004 in Japan. The cepA gene was distributed primarily in Bacteroides fragilis. Gene cfxA was detected in about 9 % of the Bacteroides isolates and 75 % of the Prevotella spp. isolates and did not appear to contribute to cephamycin resistance. Two strains of B. fragilis contained the metallo-β-lactamase gene cfiA, but they did not produce the protein product. Gene tetQ was detected in about 81, 44, and 63 % of B. fragilis isolates, other Bacteroides spp., and Prevotella spp. isolates, respectively. The ermF gene was detected in 25, 13, 56, 64, and 16 % of Bacteroides spp., Prevotella spp., Fusobacterium spp., B. wadsworthia, and anaerobic cocci, respectively. Gene mefA was found in only 10 % of the B. fragilis strains and 3 % of the non-B. fragilis strains. Genes nim and ermB were not detected in any isolate. Substitution at position 82 (Ser to Phe) in gyrA was detected in B. fragilis isolates that were less susceptible or resistant to moxifloxacin. This study is the first report on the distribution of resistance genes in anaerobes isolated from intra-abdominal infections in Japan. We expect that the results might help in understanding the resistance mechanisms of specific anaerobes.
authors: United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, Gifu, Japan.
authors: Chau Minh
authors: CM
authors: Tran
authors: Kaori
authors: K
authors: Tanaka
authors: Kunitomo
authors: K
authors: Watanabe
mesh_terms: D001419:Bacteria / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D001421:Bacteria, Anaerobic / Q000187:drug effects* / Q000235:genetics*; D001424:Bacterial Infections / Q000382:microbiology*; D001426:Bacterial Proteins / Q000235:genetics; D027081:DNA Gyrase / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005798:Genes, Bacterial; D006801:Humans; D059413:Intraabdominal Infections / Q000382:microbiology*; D007564:Japan; D008826:Microbial Sensitivity Tests; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D001618:beta-Lactamases
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; D027081:DNA Gyrase