title: Assembly of matched alpha/beta subunits to HLA class II peptide receptors.
abstract: Human antigen presenting cells express three human leukocyte antigen (HLA) class II isotypes (DR, DP, and DQ), which are composed of polymorphic α and β subunits. The combination of polymorphic α- and β-chains results in cis (encoded on the same chromosome) or trans (encoded on different chromosomes) combinations. Since some of the α-β combinations may yield mismatched non-functional α-β heterodimers, it is not entirely clear which type of HLA class II peptide receptors are found on the cell surface of antigen presenting cells. We have developed a combination of biochemical techniques for inspection of the assembly and intracellular transport of isotype matched and mismatched class II heterodimers.
authors: Institute of Molecular Cardiology, University of Düsseldorf, Düsseldorf, Germany. sebastian.temme@uni-duesseldorf.de.|Division of Immunobiology, Institute of Genetics, University of Bonn, Bonn, Germany. sebastian.temme@uni-duesseldorf.de.
authors: Sebastian
authors: S
authors: Temme
authors: Division of Immunobiology, Institute of Genetics, University of Bonn, Bonn, Germany.
authors: Nadine
authors: N
authors: Kämper
authors: Division of Immunobiology, Institute of Genetics, University of Bonn, Bonn, Germany.
authors: Norbert
authors: N
authors: Koch
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005434:Flow Cytometry; D006681:HLA-D Antigens / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D010446:Peptide Fragments / Q000378:metabolism; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D018000:Receptors, Peptide / Q000378:metabolism*; D014162:Transfection
chemical_list: D006681:HLA-D Antigens; D010446:Peptide Fragments; D018000:Receptors, Peptide