title: Verification and spatial localization of aquaporin-5 in the ocular lens.
abstract: Until recently, the lens was thought to express only two aquaporin (AQP) water channels, AQP1 and AQP0. In this study we confirm lenticular AQP5 protein expression by Western blotting and mass spectrometry in lenses from a variety of species. In addition, confocal microscopy was used to map cellular distributions of AQP5 in mouse, rat and human lenses. Tandem mass spectrometry of a human lens membrane preparation revealed extensive sequence coverage (56.2%) of AQP5. Western blotting performed on total fiber cell membranes from mouse, rat, bovine and human lenses confirmed AQP5 protein expression is conserved amongst species. Western blotting of dissected lens fractions suggests that AQP5 is processed in the lens core by C-terminal truncation. Immunohistochemistry showed that AQP5 signal was most abundant in the lens outer cortex and decreased in intensity in the lens core. Furthermore, AQP5 undergoes differentiation-dependent changes in subcellular location from an intracellular localization in differentiating fiber cells to the plasma membrane of mature fiber cells upon the loss of fiber cell nuclei. Our results show that AQP5 is a significant component of lens fiber cell membranes, representing the second most abundant water channel in these cells. Together, the changes to AQP5 distribution and structure are likely to modulate the functional role of AQP5 in different regions of the lens.
authors: Department of Optometry and Vision Science, New Zealand National Eye Centre, University of Auckland, Auckland, New Zealand.
authors: Angus C
authors: AC
authors: Grey
authors: Kerry L
authors: KL
authors: Walker
authors: Rosica S
authors: RS
authors: Petrova
authors: Jun
authors: J
authors: Han
authors: Phillip A
authors: PA
authors: Wilmarth
authors: Larry L
authors: LL
authors: David
authors: Paul J
authors: PJ
authors: Donaldson
authors: Kevin L
authors: KL
authors: Schey
mesh_terms: D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D051402:Aquaporin 5 / Q000032:analysis*; D015153:Blotting, Western; D002417:Cattle; D002454:Cell Differentiation; D006801:Humans; D007150:Immunohistochemistry; D007908:Lens, Crystalline / Q000737:chemistry* / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D008875:Middle Aged; D008969:Molecular Sequence Data; D021381:Protein Transport; D040901:Proteomics / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry
chemical_list: C496036:AQP5 protein, human; C496038:Aqp5 protein, mouse; C496040:Aqp5 protein, rat; D051402:Aquaporin 5