title: Identification of ophiobolin F synthase by a genome mining approach: a sesterterpene synthase from Aspergillus clavatus.
abstract: During a screening of putative diterpene synthase genes found in public databases using the Aspergillus oryzae expression system, it was found that a single transformant with the ACLA_76850 gene from A. clavatus produced a sesterterpene alcohol, ophiobolin F, and three minor sesterterpene hydrocarbons. The sesterterpene synthase has two catalytically independent domains (prenyltransferase/terpene cyclase) which are homologous to those of diterpene synthase, fusicoccadiene synthase. Coevolution of both domains and reaction mechanisms of these terpene synthases are discussed.
authors: Division of Chemistry, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan.
authors: Ryota
authors: R
authors: Chiba
authors: Atsushi
authors: A
authors: Minami
authors: Katsuya
authors: K
authors: Gomi
authors: Hideaki
authors: H
authors: Oikawa
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000302:isolation & purification* / Q000378:metabolism; D001230:Aspergillus / Q000737:chemistry / Q000201:enzymology / Q000235:genetics*; D015394:Molecular Structure; D054830:Sesterterpenes / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D054830:Sesterterpenes; D019883:Alkyl and Aryl Transferases; C581422:ophiobolin F synthase, Aspergillus clavatus; C108877:terpene synthase