title: Identification of a novel subtype of feline immunodeficiency virus in a population of naturally infected felines in the Brazilian Federal District.
abstract: The feline immunodeficiency virus (FIV) is a retrovirus that is found worldwide, and it can be assigned to six subtypes (A, B, C, D, E, and a putative subtype F) based on sequencing analysis of the env and gag genes. Subtypes A and B are the most common worldwide. In Brazil, several authors have isolated only subtype B, and its prevalence differs markedly among investigated populations. Blood samples from 200 domestic felines from the Federal District in Brazil were analyzed by PCR. Samples that tested positive for FIV were then cloned, sequenced, and analyzed phylogenetically and statistically. The results represent the first description of FIV infection in the Central Region of Brazil and suggest that only 2 % of felines in this region are positive for the virus. In addition, the analysis showed that one out of the four positive samples that we detected could not be assigned to any of the six classical subtypes. This sample was taken as a putative novel subtype of the FIV virus. The remaining three positive samples were assigned to subtype B, with differences existing among these samples.
authors: Laboratório de Microbiologia e Patologia Veterinária, Faculdade de Agronomia e Medicina Veterinária, Universidad de Brasília(UnB), Brasília, Brazil.
authors: T G
authors: TG
authors: Marçola
authors: C P C
authors: CP
authors: Gomes
authors: P A
authors: PA
authors: Silva
authors: G R
authors: GR
authors: Fernandes
authors: G R
authors: GR
authors: Paludo
authors: R W
authors: RW
authors: Pereira
mesh_terms: D000818:Animals; D001769:Blood / Q000821:virology; D001938:Brazil; D002371:Cat Diseases / Q000821:virology*; D002415:Cats; D003001:Cloning, Molecular; D016000:Cluster Analysis; D005838:Genotype; D016089:Immunodeficiency Virus, Feline / Q000145:classification* / Q000302:isolation & purification*; D016180:Lentivirus Infections / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology