title: Lack of reliability of nanotechnology in the of free plasma DNA in samples of patients with prostate cancer.
abstract: BACKGROUND
  Several studies seek biological markers that give diagnostic and degree of tumor development. The aim of this study was to validate the determination of plasma DNA using nanotechnology (Nanovue™-NV) in samples of 80 patients with prostate cancer.
  
  
  METHODS
  Blood samples of 80 patients of the Urology Ambulatory of Faculdade de Medicina do ABC with prostate cancer confirmed by anatomical-pathology criteria were analyzed. DNA extraction was performed using a GFX TM kit (Amersham Pharmacia Biotech, Inc, USA) following the adapted protocol. Plasma was subjected to centrifugation.
  
  
  RESULTS
  There was a big difference between the first and the second value obtained by NanoVue Only two samples had no differences between duplicates. Maximum difference between duplicates was 38 μg/mL. Average variation between 51 samples was 10.29 μg/mL, although 21 samples had differences above this average. No correlation was observed between pDNA obtained by traditional spectrophotometry and by nanotechnology.
  
  
  CONCLUSIONS
  Determination of plasma DNA by nanotechnology was not reproducible.
authors: Laboratório de Análises Clínicas, Faculdade de Medicina do ABC, Av, Príncipe de Gales 821, Santo André, CEP: 09060-650, Brazil. fon_fonseca@yahoo.com.br.
authors: Ricardo
authors: R
authors: Moreno
authors: Pamela O
authors: PO
authors: Delgado
authors: Patrícia G
authors: PG
authors: Coelho
authors: Sarah R
authors: SR
authors: Marsicano
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authors: Fonseca