title: Testing robustness of relative complexity measure method constructing robust phylogenetic trees for Galanthus L. using the relative complexity measure.
abstract: BACKGROUND
  Most phylogeny analysis methods based on molecular sequences use multiple alignment where the quality of the alignment, which is dependent on the alignment parameters, determines the accuracy of the resulting trees. Different parameter combinations chosen for the multiple alignment may result in different phylogenies. A new non-alignment based approach, Relative Complexity Measure (RCM), has been introduced to tackle this problem and proven to work in fungi and mitochondrial DNA.
  
  
  RESULTS
  In this work, we present an application of the RCM method to reconstruct robust phylogenetic trees using sequence data for genus Galanthus obtained from different regions in Turkey. Phylogenies have been analyzed using nuclear and chloroplast DNA sequences. Results showed that, the tree obtained from nuclear ribosomal RNA gene sequences was more robust, while the tree obtained from the chloroplast DNA showed a higher degree of variation.
  
  
  CONCLUSIONS
  Phylogenies generated by Relative Complexity Measure were found to be robust and results of RCM were more reliable than the compared techniques. Particularly, to overcome MSA-based problems, RCM seems to be a reasonable way and a good alternative to MSA-based phylogenetic analysis. We believe our method will become a mainstream phylogeny construction method especially for the highly variable sequence families where the accuracy of the MSA heavily depends on the alignment parameters.
authors: Department of Biology, Abant İzzet Baysal University, Bolu 14280, Turkey. bakis_y@ibu.edu.tr
authors: Yasin
authors: Y
authors: Bakış
authors: Hasan H
authors: HH
authors: Otu
authors: Nivart
authors: N
authors: Taşçı
authors: Cem
authors: C
authors: Meydan
authors: Neş'e
authors: N
authors: Bilgin
authors: Sırrı
authors: S
authors: Yüzbaşıoğlu
authors: O Uğur
authors: OU
authors: Sezerman
mesh_terms: D018742:DNA, Chloroplast / Q000737:chemistry; D018744:DNA, Plant / Q000737:chemistry; D021903:DNA, Ribosomal Spacer / Q000737:chemistry; D027801:Galanthus / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D018742:DNA, Chloroplast; D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer