title: A heteroglycan from the mycelia of Pleurotus ostreatus: structure determination and study of antioxidant properties.
abstract: A heteroglycan (PS) isolated from the mycelia of Pleurotus ostreatus was found to consist of l-fucose, d-mannose, and d-glucose in a molar ratio of nearly 1:2:3. On the basis of acid hydrolysis, methylation analysis, periodate oxidation, and NMR studies (1H, 13C, TOCSY, DQF-COSY, NOESY, ROESY, HSQC, HMBC, and DEPT), the structure of the repeating unit of the PS was determined. The repeating unit had a branched backbone composed of (1→6)-linked α and βd-glucose and (1→2)-linked α-l-fucose. Branching occurred at C-4 position of (1→6)-linked α-d-glucopyranosyl residue with terminal β-d-mannose and C-3 position of (1→6)-linked β-d-glucopyranosyl residue with (1→6)-α-d-mannopyranosyl moiety terminated by α-d-glucose. The structure of the repeating unit of the PS was proposed as: [formula: see text]. The antioxidant properties of the PS were studied. The scavenging activity of hydroxyl free radicals whose EC50 value of PS at 943 μg/mL was determined. Further, EC50 value of the PS at 53 μg/mL was observed for scavenging activity of superoxide free radicals. Chelating effects (54.82%) of ferrous ions were observed at 1mg/mL of the PS.
authors: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors: Sukesh
authors: S
authors: Patra
authors: Pradip
authors: P
authors: Patra
authors: Kankan K
authors: KK
authors: Maity
authors: Soumitra
authors: S
authors: Mandal
authors: Sanjay K
authors: SK
authors: Bhunia
authors: Biswajit
authors: B
authors: Dey
authors: K Sanjana P
authors: KS
authors: Devi
authors: Somanjana
authors: S
authors: Khatua
authors: Krishnendu
authors: K
authors: Acharya
authors: Tapas K
authors: TK
authors: Maiti
authors: Syed S
authors: SS
authors: Islam
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D025282:Mycelium / Q000737:chemistry*; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D011134:Polysaccharides