title: [In situ visual imaging and in vivo distribution of head and neck squamous cell carcinoma in mice by near-infrared fluorescent quantum dots epidermal growth factor receptor monoclonal antibody probe].
abstract: OBJECTIVE
  To investigate the in situ visualization imaging and the in vivo distribution of the near-infrared fluorescent quantum dots (QDs) epidermal growth factor receptor (EGFR) monoclonal antibody (mAb) probe in head and neck squamous cell carcinoma (HNSCC).
  
  
  METHODS
  QDs with an emission wavelength of 800 nm (QD800) was conjugated with EGFR mAb to produce QD800-EGFR mAb. QD800-EGFR mAb was co-cultured with BcaCD885 squamous cancer cell line for 30 min and observed with laser scanning confocal microscope (LSCM). QD800-EGFR mAb was injected into HNSCC animal model through the tail vein, and the in situ visualization imaging and the in vivo distribution of QD800-EGFR mAb was analyzed at different time points.
  
  
  RESULTS
  BcaCD885 squamous cell carcinoma in the head and neck can be imaged clearly and visually after intravenous injection of QD800-EGFR mAb probe, these fluorescence signals lasted for 24 h. The most complete tumor images with maximal signal-to-noise ratio were observed from 30 min to 6 h after injection of the probe. In vivo tissue distribution studies demonstrated that QD800 aggregated mostly in liver. QD800 aggregation decreased with time in tumors, and QD800 didn't aggregate in heart, brain, intestine, lung and stomach.
  
  
  CONCLUSIONS
  The QD800-EGFR mAb probe can clearly produce visual images in head and neck cancer. It has promising prospects in visualization in vivo imaging and individual treatment of head and neck cancer.
authors: Dept. of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors: Hong
authors: H
authors: Tang
authors: Kai
authors: K
authors: Yang
authors: Cheng
authors: C
authors: Zhao
authors: Yunlong
authors: Y
authors: Bai
authors: Hao
authors: H
authors: Huang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002294:Carcinoma, Squamous Cell; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D066246:ErbB Receptors; D005456:Fluorescent Dyes; D006258:Head and Neck Neoplasms; D051379:Mice; D008819:Mice, Nude; D045663:Quantum Dots; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D066246:ErbB Receptors