title: Preparation of Pt/polypyrrole-para toluene sulfonate hydrogen peroxide sensitive electrode for the utilizing as a biosensor.
abstract: A film electrode with electropolymerization of pyrrole (Py) and para-toluene sulfonate (pTS) as a anionic dopant is prepared and its sensitivity to hydrogen peroxide is investigated. The polypyrrole is deposited on a 0.5 cm(2) Pt plate an electrochemically prepared pTS ion-doped polypyrrole film by scanning the electrode potential between - 0.8 and + 0.8 V at a scan rate of 20 mV/s. The electrode's sensitivity to hydrogen peroxide is investigated at room temperature using 0.1 M phosphate buffer at pH 7.5. The working potential is found as a 0.3 V. The concentrations of pyrrole and pTS are 50mM M and 25 mM. Polypyrrole was coated on the electrode surface within 10 cycles. İmmobilization of glucose oxidase carried out on Pt/polypyrrole-para toluene sulfonate (Pt/PPy-pTS) film by cross-linking with glutaraldehyde. The morphology of electrodes was characterized by SEM and AFM. Moreover, contact angle measurements were made with 1 μL water of polymer film and enzyme electrode. It has shown that enzyme electrode is very sensitive against to glucose.
authors: Department of Chemistry, Faculty of Sciences, Gazi University , Ankara , Turkey.
authors: Servet
authors: S
authors: Çete
authors: Özgür
authors: Ö
authors: Bal
mesh_terms: D000818:Animals; D001557:Benzenesulfonates / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D004563:Electrochemistry; D004566:Electrodes; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D005976:Glutaral / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry; D010984:Platinum / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014050:Toluene / Q000737:chemistry*
chemical_list: D001557:Benzenesulfonates; D011108:Polymers; D011758:Pyrroles; D012710:Serum Albumin, Bovine; C067635:polypyrrole; D014050:Toluene; D010984:Platinum; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; C029501:4-toluenesulfonic acid; D005976:Glutaral