title: Inhibitory effects of black soybean on platelet activation mediated through its active component of adenosine.
abstract: Owing to the beneficial health effects on human cardiovascular system, soybeans and soy-related products have been a focus of intensive research. Soy isoflavones are known to be primarily responsible for the soy-related biological effects including anti-platelet activity but its in vivo relevancy has not been fully verified. Here we compared the role of adenosine, an active ingredient abundant in black soybean (BB) extract, in the anti-platelet effects of BB, to that of soy isoflavones. At the concentrations existing in BB, isoflavones such as genistein and daidzein could not attenuate collagen-induced platelet aggregation, however, adenosine significantly inhibited platelet aggregation with an equivalent potency to BB, suggesting that adenosine may be the major bioactive component. Consistently, the anti-aggregatory effects of BB disappeared after treatment of adenosine receptor antagonists. The effects of BB are mediated by adenosine through intracellular cAMP and subsequent attenuation of calcium mobilization. Of note, adenosine and BB significantly reduced platelet fibrinogen binding and platelet adhesion, other critical events for platelet activation, which were not affected by isoflavones. Taken together, we demonstrated that adenosine might be the major active ingredient for BB-induced anti-platelet activity, which will shed new light on the roles of adenosine as a bioactive compound in soybeans and soy-related food.
authors: College of Pharmacy, Seoul National University, Seoul 151-742, Republic of Korea.
authors: Keunyoung
authors: K
authors: Kim
authors: Kyung-Min
authors: KM
authors: Lim
authors: Hyun-Jung
authors: HJ
authors: Shin
authors: Dae-Bang
authors: DB
authors: Seo
authors: Ji-Yoon
authors: JY
authors: Noh
authors: Seojin
authors: S
authors: Kang
authors: Han Young
authors: HY
authors: Chung
authors: Sue
authors: S
authors: Shin
authors: Jin-Ho
authors: JH
authors: Chung
authors: Ok-Nam
authors: ON
authors: Bae
mesh_terms: D000241:Adenosine / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D001792:Blood Platelets / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion; D003094:Collagen / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D015539:Platelet Activation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D015398:Signal Transduction; D030262:Soybean Proteins / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry*; D055815:Young Adult
chemical_list: D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; D030262:Soybean Proteins; D003094:Collagen; D000242:Cyclic AMP; D000241:Adenosine; D002118:Calcium