title: TACTICS, an interactive platform for customized high-content bioimaging analysis.
abstract: We describe a modular MATLAB® Toolbox named TACTICS for time-lapse image analysis that meets several requirements not generally offered by currently available software packages: (i) the ability to assess quality of extracted imaging information by directly linking data end points to the original position, (ii) massively parallel analysis of each parameter, for flow cytometry-like assessment of possible relationships between parameters within sub-populations of the images, (iii) options for user control of the tracking such as an interface to restrict the analysis region, (iv) manual correction of automated processes and (v) user interfaces for post-tracking analysis that is linked to the original images, including options to view cell pedigrees and normalized polarization ratios based on fluorescence ratiometric measurements.
authors: Immune Signalling Laboratory, Peter MacCallum Cancer Centre, East Melbourne 3002, Australia.
authors: Raz
authors: R
authors: Shimoni
authors: Kim
authors: K
authors: Pham
authors: Mohammed
authors: M
authors: Yassin
authors: Min
authors: M
authors: Gu
authors: Sarah M
authors: SM
authors: Russell
mesh_terms: D007091:Image Processing, Computer-Assisted / Q000379:methods*; D012984:Software; D059008:Time-Lapse Imaging / Q000379:methods*