title: Production of primary human CD4⁺ T cell lines and clones.
abstract: Tetramer staining of CD4(+) T cells is a valuable technique in immunology for detecting rare autoreactive T cells. Generating clones or cell lines from autoantigen tetramer-positive CD4(+) T cells allows further characterization and phenotyping of autoreactive cells.
authors: Benaroya Research Institute, Seattle, WA, USA.
authors: Jessica
authors: J
authors: Matthis
authors: Benaroya Research Institute, Seattle, WA, USA. reijonen@benaroyaresearch.org.
authors: Helena
authors: H
authors: Reijonen
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D049109:Cell Proliferation; D002469:Cell Separation; D002999:Clone Cells / Q000166:cytology; D006801:Humans