title: Xyloglucan endotransglycosylase/hydrolase genes from a susceptible and resistant jute species show opposite expression pattern following Macrophomina phaseolina infection.
abstract: Two of the most widely and intensively cultivated jute species, Corchorus capsularis and Corchorus olitorius, suffer severely from a stem rot disease caused by the fungus Macrophomina phaseolina. Wild jute species, C. trilocularis, shows resistance to this pathogenic fungus. In this study, the technique of differential display was applied to identify genes which are differentially expressed, under both infected and un-infected conditions, between C. trilocularis and C. olitorius var O-72. Two xyloglucan endotransglycosylase/hydrolase (XTH) genes designated CoXTH1 (from Corchorus olitorius) and CtXTH1 (from C.trilocularis) were identified from each of the two species which show different expression patterns upon fungal infection. A steady rise in the expression of CtXTH1 in response to infection was observed by quantitative real time PCR whereas the expression of CoXTH1 was found to be downregulated. Full length sequences of these two genes were determined using primer based gene walking and RACE PCR. This study confirms the involvement of XTH in molecular interactions between M. phaseolina and jute. However, it remains to be explored whether XTH is an essential component of the signaling pathway involved in plant-fungal interaction.
authors: Molecular Biology Lab; Department of Biochemistry and Molecular Biology; University of Dhaka; Dhaka, Bangladesh.
authors: Sazia
authors: S
authors: Sharmin
authors: Muhammad Shafiul
authors: MS
authors: Azam
authors: Md Shahidul
authors: MS
authors: Islam
authors: Abu Ashfaqur
authors: AA
authors: Sajib
authors: Niaz
authors: N
authors: Mahmood
authors: A M Mahedi
authors: AM
authors: Hasan
authors: Razib
authors: R
authors: Ahmed
authors: Kishwar
authors: K
authors: Sultana
authors: Haseena
authors: H
authors: Khan
keywords: Macrophomina phaseolina; XTH; differential display; jute