title: Plant coilin: structural characteristics and RNA-binding properties.
abstract: Cajal bodies (CBs) are dynamic subnuclear compartments involved in the biogenesis of ribonucleoproteins. Coilin is a major structural scaffolding protein necessary for CB formation, composition and activity. The predicted secondary structure of Arabidopsis thaliana coilin (Atcoilin) suggests that the protein is composed of three main domains. Analysis of the physical properties of deletion mutants indicates that Atcoilin might consist of an N-terminal globular domain, a central highly disordered domain and a C-terminal domain containing a presumable Tudor-like structure adjacent to a disordered C terminus. Despite the low homology in amino acid sequences, a similar type of domain organization is likely shared by human and animal coilin proteins and coilin-like proteins of various plant species. Atcoilin is able to bind RNA effectively and in a non-specific manner. This activity is provided by three RNA-binding sites: two sets of basic amino acids in the N-terminal domain and one set in the central domain. Interaction with RNA induces the multimerization of the Atcoilin molecule, a consequence of the structural alterations in the N-terminal domain. The interaction with RNA and subsequent multimerization may facilitate coilin's function as a scaffolding protein. A model of the N-terminal domain is also proposed.
authors: A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia.
authors: Valentine
authors: V
authors: Makarov
authors: Daria
authors: D
authors: Rakitina
authors: Anna
authors: A
authors: Protopopova
authors: Igor
authors: I
authors: Yaminsky
authors: Alexander
authors: A
authors: Arutiunian
authors: Andrew J
authors: AJ
authors: Love
authors: Michael
authors: M
authors: Taliansky
authors: Natalia
authors: N
authors: Kalinina
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites / Q000235:genetics; D006801:Humans; D008958:Models, Molecular; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding / Q000235:genetics; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary / Q000235:genetics; D018749:RNA, Plant / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C513350:Atcoilin protein, Arabidopsis; D009687:Nuclear Proteins; D018749:RNA, Plant; D016601:RNA-Binding Proteins; C068921:p80-coilin