title: Inhibitory effect of linalool-rich essential oil from Lippia alba on the peptidase and keratinase activities of dermatophytes.
abstract: Abstract Lippia alba (Miller) N.E. Brown is an aromatic plant known locally as "Erva-cidreira-do-campo" that has great importance in Brazilian folk medicine. The aim of our study was to evaluate the antidermatophytic potential of linalool-rich essential oil (EO) from L. alba and analyze the ability of this EO to inhibit peptidase and keratinase activities, which are important virulence factors in dermatophytes. The minimum inhibitory concentrations (MICs) of L. alba EO were 39, 156 and 312 µg/mL against Trichophyton rubrum, Epidermophyton floccosum and Microsporum gypseum, respectively. To evaluate the influence of L. alba EO on the proteolytic and keratinolytic activities of these dermatophytes, specific inhibitory assays were performed. The results indicated that linalool-rich EO from L. alba inhibited the activity of proteases and keratinases secreted from dermatophytes, and this inhibition could be a possible mechanism of action against dermatophytes. Due to the effective antidermatophytic activity of L. alba EO, further experiments should be performed to explore the potential of this linalool-rich EO as an alternative antifungal therapy.
authors: Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro , Rio de Janeiro , Brazil and.
authors: Danielle Cristina Machado
authors: DC
authors: Costa
authors: Alane Beatriz
authors: AB
authors: Vermelho
authors: Catia Amancio
authors: CA
authors: Almeida
authors: Edilma Paraguai
authors: EP
authors: de Souza Dias
authors: Sabrina Martins Lage
authors: SM
authors: Cedrola
authors: Maria de Fátima
authors: Mde F
authors: Arrigoni-Blank
authors: Arie Fitzgerald
authors: AF
authors: Blank
authors: Celuta Sales
authors: CS
authors: Alviano
authors: Daniela Sales
authors: DS
authors: Alviano
mesh_terms: D000080462:Acyclic Monoterpenes; D003883:Arthrodermataceae / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D032411:Lippia / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D039821:Monoterpenes / Q000032:analysis*; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D010447:Peptide Hydrolases / Q000187:drug effects*; D011480:Protease Inhibitors / Q000494:pharmacology*
chemical_list: D000080462:Acyclic Monoterpenes; D039821:Monoterpenes; D009822:Oils, Volatile; D011480:Protease Inhibitors; C018584:linalool; D010447:Peptide Hydrolases; C035855:keratinase