title: A new approach for pharmacokinetic studies of natural products: measurement of isoliquiritigenin levels in mice plasma, urine and feces using modified automated dosing/blood sampling system.
abstract: The present study was undertaken to investigate the pharmacokinetics of isoliquiritigenin (isoLQ) as determined by the automated dosing/blood sampling (ABS) and traditional manual blood sampling techniques in awake and freely moving mice using combined liquid chromatography tandem mass spectrometry. Pharmacokinetic comparison was conducted by allocating mice into two groups; an ABS group (intravenous study and oral studies, n = 5 each) and a manual group (intravenous and oral studies; n = 5 each). Significant differences in pharmacokinetic parameters (area under the curve and clearances) were observed between ABS and manual groups. This could be mainly due to the blood sampling site difference (via heart puncture in traditional manual group and via carotid artery in ABS groups). The low F of isoLQ could be mainly due to a considerable gastrointestinal and/or hepatic first-pass effect and not to incomplete absorption. The driving force for distribution and elimination of drugs is its concentration in the arterial blood. Therefore, the ABS method was found to be a useful drug development tool for accelerating the process of preclinical in vivo studies and for obtaining reliable and accurate pharmacokinetic parameters in mice.
authors: College of Pharmacy, Dongguk University-Seoul, 32 Dongguk-lo, Ilsandong-gu, Goyang, Gyeonggi-do, 410-820, South Korea.
authors: Seung Yon
authors: SY
authors: Han
authors: Young-Won
authors: YW
authors: Chin
authors: Young Hee
authors: YH
authors: Choi
mesh_terms: D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D001331:Automation / Q000379:methods*; D001800:Blood Specimen Collection; D047188:Chalcones / Q000008:administration & dosage / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics*; D002853:Chromatography, Liquid / Q000379:methods; D005243:Feces / Q000737:chemistry; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015203:Reproducibility of Results; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D047188:Chalcones; C040920:isoliquiritigenin