title: Determination of C5a in murine models of staphylococcal enterotoxin B-induced toxic shock.
abstract: Robust host innate immune response to staphylococcal enterotoxin B (SEB) and structurally related superantigens causes toxic shock and various autoimmune diseases. While proinflammatory cytokines are known for mediating SEB-induced toxicity, the role of complement C5a in SEB-mediated shock is less well-understood. An ELISA was developed to measure the complement activation product, C5a, in different murine models of toxic shock. This assay provides easy, quantifiable data for complement activation and its role in various SEB-induced toxic shock models.
authors: Integrated Toxicology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA. teresa.krakauer@us.army.mil
authors: Teresa
authors: T
authors: Krakauer
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D003182:Complement C5 / Q000032:analysis* / Q000276:immunology; D004195:Disease Models, Animal; D004768:Enterotoxins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006735:Horseradish Peroxidase / Q000737:chemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D012772:Shock, Septic / Q000097:blood* / Q000276:immunology; D013203:Staphylococcal Infections / Q000097:blood* / Q000276:immunology; D018089:Superantigens / Q000276:immunology*
chemical_list: D000906:Antibodies; D003182:Complement C5; D004768:Enterotoxins; D018089:Superantigens; C031818:enterotoxin B, staphylococcal; D006735:Horseradish Peroxidase