title: Poria cocos inhibits high glucose-induced proliferation of rat mesangial cells.
abstract: Mesangial cell proliferation is correlated with the progression of renal failure. The purpose of this study was to determine whether a water extract of Poria cocos Wolf (WPC), a well-known medicinal plant, regulates rat mesangial cell proliferation in the presence of high glucose (HG). HG significantly accelerated [(3)H]-thymidine incorporation, which was inhibited by WPC (1-50 μg/mL) in a dose-dependent manner. Cell migration and fibronectin mRNA expression data also supported the anti-proliferative effect of WPC. Western blot analysis revealed that pretreatment with WPC decreased the expression of cyclins and cyclin-dependent kinases (CDKs) and promoted the expression of p21(waf1/cip1) and p27(kip1). WPC also suppressed HG-induced p38 mitogen-activated protein kinase (p38 MAPK) and extracellular-signal-regulated kinase 1/2 (ERK 1/2) phosphorylation. Furthermore, WPC inhibited HG-induced production of dichlorofluorescein (DCF)-sensitive intracellular reactive oxygen species (ROS). In conclusion, HG promoted mesangial cell proliferation, and WPC inhibited this activity, at least in part, via induction of cell cycle arrest and activation of anti-oxidant properties. Taken together, these results suggest that P. cocos may be a potent regulator of HG-induced proliferation.
authors: College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, Jeonbuk 570-749, Republic of Korea.
authors: Jung Joo
authors: JJ
authors: Yoon
authors: Yun Jung
authors: YJ
authors: Lee
authors: So Min
authors: SM
authors: Lee
authors: Song Nan
authors: SN
authors: Jin
authors: Dae Gill
authors: DG
authors: Kang
authors: Ho Sub
authors: HS
authors: Lee
mesh_terms: D000818:Animals; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D016213:Cyclins / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000009:adverse effects* / Q000037:antagonists & inhibitors*; D020935:MAP Kinase Signaling System; D008297:Male; D050527:Mesangial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010766:Phosphorylation; D010936:Plant Extracts / Q000494:pharmacology*; D055441:Poria; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014867:Water; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D016213:Cyclins; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D014867:Water; D018844:Cyclin-Dependent Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D005947:Glucose; D013936:Thymidine