title: Effect of radiation on the Notch signaling pathway in osteoblasts.
abstract: Notch signaling has been shown to be important in osteoblast differentiation.             Therapeutic radiation has been shown to alter the skeletal system, yet little             information is available on the changes in Notch signaling in irradiated osteoblasts.             The purpose of this study was to analyze the effect of radiation therapy with             2 and 4 Gy on Notch signaling in osteoblasts. In order to assess the radiation             damage on osteoblast differentiation, total RNA and protein were collected three             days after exposure to radiation. The effects of radiation on Notch signaling             at the early and terminal stages of osteoblastic MC3T3-E1 cell differentiation             was analyzed by qRT-PCR and western blot analysis. Our study applied a previously             established method to induce MC3T3-E1 cell differentiation into osteoblasts and             osteoblast precursors. Our results showed that the expression of Notch receptors             (Notch1-4), ligands (Jagged1, Jagged2 and Delta1), target of Notch signaling (Hes1)             and markers (ALP, M-CSF, RANKL and OPG) were altered following 2 and 4 Gy of irradiation.             The present research did not indicate a strong relationship between Notch1 regulation             and suppression of osteoblast differentiation. We found Hes1 may play a role in             the radiation effect on osteoblast differentiation. Our results indicate that             radiated osteoblast precursors and osteoblasts promoted osteoclast differentiation             and proliferation.
authors: Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, PR China.
authors: Bing
authors: B
authors: Yang
authors: Quan
authors: Q
authors: Tang
authors: Janine
authors: J
authors: Post
authors: Hui
authors: H
authors: Zhou
authors: Xiao-Bin
authors: XB
authors: Huang
authors: Xiao-Dong
authors: XD
authors: Zhang
authors: Qin
authors: Q
authors: Wang
authors: Yuan-Ming
authors: YM
authors: Sun
authors: Fei-Yue
authors: FY
authors: Fan
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000528:radiation effects; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000096:biosynthesis / Q000528:radiation effects; D002135:Calcium-Binding Proteins / Q000096:biosynthesis / Q000528:radiation effects; D002454:Cell Differentiation / Q000528:radiation effects; D002460:Cell Line; D005720:Gamma Rays / Q000627:therapeutic use; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000528:radiation effects; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis / Q000528:radiation effects; D047908:Intracellular Signaling Peptides and Proteins / Q000096:biosynthesis / Q000528:radiation effects; D000072100:Jagged-1 Protein; D000072101:Jagged-2 Protein; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis / Q000528:radiation effects; D008565:Membrane Proteins / Q000096:biosynthesis / Q000528:radiation effects; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D053244:Osteoprotegerin / Q000096:biosynthesis / Q000528:radiation effects; D053245:RANK Ligand / Q000096:biosynthesis / Q000528:radiation effects; D051880:Receptors, Notch / Q000378:metabolism / Q000528:radiation effects*; D000072099:Serrate-Jagged Proteins; D015398:Signal Transduction / Q000528:radiation effects*; D000072056:Transcription Factor HES-1
chemical_list: D000469:Alkaline Phosphatase; D051792:Basic Helix-Loop-Helix Proteins; D002135:Calcium-Binding Proteins; D018398:Homeodomain Proteins; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D000072101:Jagged-2 Protein; D016173:Macrophage Colony-Stimulating Factor; D008565:Membrane Proteins; D053244:Osteoprotegerin; D053245:RANK Ligand; D051880:Receptors, Notch; D000072099:Serrate-Jagged Proteins; D000072056:Transcription Factor HES-1; C489734:Hes1 protein, mouse; C000606274:Jag1 protein, mouse; C490503:Jag2 protein, mouse; C506031:Tnfrsf11b protein, mouse; C505689:Tnfsf11 protein, mouse; C064045:delta protein