title: Expression of XPG protein in human normal and tumor tissues.
abstract: XPG (Xeroderma pigmentosum group G complementing factor) is a protein associated with DNA repair and transcription. Point mutations in ERCC5, the gene coding for XPG, cause the cancer-prone disorder xeroderma pigmentosum (XP) while truncation mutations give rise to individuals with the combined clinical features of XP and Cockayne syndrome. Polymorphisms of ERCC5 or alterations in XPG mRNA expression were also associated to an increase risk of different cancers types and to prognosis of cancer patients. However, the expression of XPG protein in different normal or tumor human tissues is not well known. In the present work, we have validated an immunohistochemistry (IHC) assay for detection of expression levels of XPG protein in FFPE human tissue samples. We have also tested this IHC assay in different normal and tumor human tissues. On a microarray containing 28 normal cores, positive staining was observed in 60% of the samples. The highest staining was detected in adrenal gland, breast, colon, heart, kidney, thyroid and tongue. In tumors, positive staining was observed in 9 of 10 breast cancer samples and in all 5 ovarian cancer and 5 sarcomas samples. Subcellular localization was predominantly nuclear. The use of this validated methodology would help to interpret the role of XPG in tumorogenesis and its use as a possible prognostic or predictive factor.
authors: Cell Biology and Pharmacogenomics Department PharmaMar SA, Colmenar Viejo, Spain. maracil@pharmamar.com
authors: Miguel
authors: M
authors: Aracil
authors: Lisa M
authors: LM
authors: Dauffenbach
authors: Marta Martínez
authors: MM
authors: Diez
authors: Rana
authors: R
authors: Richeh
authors: Victoria
authors: V
authors: Moneo
authors: Juan Fernando Martínez
authors: JF
authors: Leal
authors: Luis Francisco García
authors: LF
authors: Fernández
authors: Christopher A
authors: CA
authors: Kerfoot
authors: Carlos M
authors: CM
authors: Galmarini
mesh_terms: D000311:Adrenal Glands / Q000502:physiology; D001940:Breast / Q000502:physiology; D001943:Breast Neoplasms / Q000235:genetics; D003057:Cockayne Syndrome / Q000235:genetics; D003106:Colon / Q000502:physiology; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D004720:Endonucleases / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D006367:HeLa Cells; D006321:Heart / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods / Q000592:standards; D007668:Kidney / Q000502:physiology; D008297:Male; D009369:Neoplasms / Q000235:genetics* / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010051:Ovarian Neoplasms / Q000235:genetics; D015203:Reproducibility of Results; D012509:Sarcoma / Q000235:genetics; D013961:Thyroid Gland / Q000502:physiology; D014059:Tongue / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D014983:Xeroderma Pigmentosum / Q000235:genetics* / Q000378:metabolism*; D000099250:DNA Excision Repair Protein ERCC-5
chemical_list: D004268:DNA-Binding Proteins; D004720:Endonucleases; D009687:Nuclear Proteins; D014157:Transcription Factors; D000099250:DNA Excision Repair Protein ERCC-5
keywords: XPG; immunohistochemistry; tumor tissue