title: Qualitative and quantitative analysis of the unsaponifiable fraction of vegetable oils by using comprehensive 2D GC with dual MS/FID detection.
abstract: The present investigation is focused on the development of a comprehensive two-dimensional GC (GC × GC) method, with dual MS/FID detection, for the qualitative and quantitative analysis of the entire unsaponifiable fraction of vegetable oils. The unsaponifiable fraction forms a minor, highly specific part of a vegetable oil, and can be used as an indicator of genuineness. The column set used consisted of a low-polarity first dimension, and a medium-polarity secondary one, both characterized by a high thermal stability. The use of dual detection enabled the attainment of both mass spectral information and relative % FID data. The complexity of the fingerprint, generated by the unsaponifiable fraction, fully justified the employment of the two-dimensional GC technology. Furthermore, two other GC × GC benefits contributed greatly to the attainment of promising results, namely sensitivity enhancement and the formation of group-type patterns. The method herein proposed could potentially open a new opportunity for the more in-depth knowledge of the unsaponifiable fraction of vegetable oils.
authors: Dipartimento Farmaco-chimico, Facoltà di Farmacia, Università degli Studi di Messina, Messina, Italy.
authors: Peter Q
authors: PQ
authors: Tranchida
authors: Simona
authors: S
authors: Salivo
authors: Flavio A
authors: FA
authors: Franchina
authors: Ivana
authors: I
authors: Bonaccorsi
authors: Paola
authors: P
authors: Dugo
authors: Luigi
authors: L
authors: Mondello
mesh_terms: D045424:Complex Mixtures / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D010840:Phytosterols / Q000032:analysis*; D010938:Plant Oils / Q000737:chemistry*; D012503:Saponins; D012680:Sensitivity and Specificity
chemical_list: D045424:Complex Mixtures; D010840:Phytosterols; D010938:Plant Oils; D012503:Saponins