title: F-actin may play an important role in IL-1β-stimulated hippocampal neurons.
abstract: OBJECTIVE
  The aim of this study was to investigate whether IL-1β treatment affects F-actin in hippocampal neurons.
  
  
  METHODS
  Primary culture of hippocampal neurons were prepared from rat embryos, and the effect of different concentrations of IL-1β on the cell phenotype was investigated. Cell viability was monitored by CCK-8 activity analysis. Western blotting and immunofluorescence were performed to analyze protein levels and morphological changes in cells treated with IL-1β.
  
  
  RESULTS
  IL-1β increased viability at low doses and these increases were severely blunted at higher concentrations. Similarly, the expression of F-actin was increased at low concentrations and decreased at high concentrations.
  
  
  CONCLUSIONS
  IL-1β has a critical role in regulating hippocampal neuron viability by stimulating F-actin expression. These data suggest that IL-1β secretion at low doses is beneficial for the expression of F-actin, which can improve the survival of hippocampal neurons during stress. However,these effect were severely blunted at higher concentrations.
authors: Department of Psychiatry, Renmin Hospital, Wuhan University, Jiefang Road 238#, Wuhan 430060, China.
authors: Xueping
authors: X
authors: Zhang
authors: Zhongxiang
authors: Z
authors: Cai
authors: Gaohua
authors: G
authors: Wang
authors: Huiling
authors: H
authors: Wang
authors: Zhongchun
authors: Z
authors: Liu
authors: Xin
authors: X
authors: Guo
authors: Can
authors: C
authors: Yang
authors: Xiaoping
authors: X
authors: Wang
authors: Hesheng
authors: H
authors: Wang
authors: Chang
authors: C
authors: Shu
authors: Ling
authors: L
authors: Xiao
mesh_terms: D000199:Actins / Q000502:physiology*; D000818:Animals; D002478:Cells, Cultured; D046149:Embryo Culture Techniques / Q000379:methods; D006624:Hippocampus / Q000378:metabolism / Q000502:physiology*; D053583:Interleukin-1beta / Q000378:metabolism / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000199:Actins; D053583:Interleukin-1beta