title: An assay suitable for high throughput screening of anti-influenza drugs.
abstract: We developed a novel drug screening system for anti-influenza A virus by targeting the M2 proton channel. In the SPP (Single Protein Production) system, E. coli cell growth occurs only in the presence of effective M2 channel inhibitors, and thus simple measurement of cell growth was used as readouts for drug screening. Two potential inhibitors for M2 (V27A) mutant were verified using this method, which inhibit both the mutant and wild-type M2 channels.
authors: Department of Biochemistry and Center for Advanced Biotechnology and Medicine, Robert Wood Johnson Medical School, Piscataway, New Jersey, United States of America.
authors: Lili
authors: L
authors: Mao
authors: Jun
authors: J
authors: Wang
authors: William F
authors: WF
authors: DeGrado
authors: Masayori
authors: M
authors: Inouye
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D024882:Drug Resistance, Viral / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D057166:High-Throughput Screening Assays; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects / Q000254:growth & development; D007251:Influenza, Human / Q000188:drug therapy*; D009154:Mutation; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D014763:Viral Matrix Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D000086082:Viroporin Proteins
chemical_list: D000998:Antiviral Agents; D011994:Recombinant Proteins; D014763:Viral Matrix Proteins; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins