title: Proteomic analysis of the lung in rats with hypobaric hypoxia-induced pulmonary hypertension.
abstract: Experimental pulmonary hypertension that develops in hypobaric hypoxia is characterized by structural remodeling of the lung. Proteomics - which may be the most powerful way to uncover unknown remodeling proteins involved in enhancing cardiovascular performance - was used to study 150 male Wistar rats housed for up to 21 days in a chamber at the equivalent of 5500 m altitude level. After 14 days' exposure to hypobaric hypoxia, pulmonary arterial pressure (PAP) was significantly increased. In lung tissue, about 140 matching protein spots were found among 8 groups (divided according to their hypobaric period) by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) (pH4.5-pH6.5, 30 kDa-100 kDa). In hypobaric rats, three spots were increased two-fold or more (vs. control rats) in two-dimensional differential in-gel electrophoresis (2D-DIGE). The increased proteins were identified, by matrix-assisted laser desorption ionization time of flight (MALDI-TOF), as one isoform of heat shock protein 70 (HSP70) and two isoforms of protein disulfide isomerase associated 3. This result was confirmed by Western blotting analysis of 2D-PAGE. Conceivably, HSP70 and PDIA3 may play roles in modulating the lung structural remodeling that occurs due to pulmonary hypertension in hypobaric hypoxia.
authors: Department of Pathology and Laboratory Medicine, National Defense Medical College, Tokorozawa, Japan.
authors: Yuichiro
authors: Y
authors: Ohata
authors: Sho
authors: S
authors: Ogata
authors: Kuniaki
authors: K
authors: Nakanishi
authors: Fumiko
authors: F
authors: Kanazawa
authors: Maki
authors: M
authors: Uenoyama
authors: Sadayuki
authors: S
authors: Hiroi
authors: Susumu
authors: S
authors: Tominaga
authors: Tosifusa
authors: T
authors: Toda
authors: Toshiaki
authors: T
authors: Kawai
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006976:Hypertension, Pulmonary / Q000378:metabolism*; D000860:Hypoxia / Q000378:metabolism*; D007150:Immunohistochemistry; D008168:Lung / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D019704:Protein Disulfide-Isomerases / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D018840:HSP70 Heat-Shock Proteins; D020543:Proteome; C513196:PDIA3 protein, rat; D019704:Protein Disulfide-Isomerases