title: Ectomesenchymal chondromyxoid tumor of the tongue: insights on histogenesis.
abstract: OBJECTIVE
  The objective of this study was to investigate the histogenesis of ectomesenchymal chondromyxoid tumors (ECTs) of the tongue.
  
  
  METHODS
  The biochemical characteristics of a rarely occurring tumor of the tongue were analyzed by immunohistochemistry and reverse-transcriptase polymerase chain reaction (RT-PCR), and its biological properties were assessed in primary culture in serum-free media.
  
  
  RESULTS
  Immunohistochemistry showed that the tumor cells were strongly positive for vimentin, S-100, and glial fibrillary acidic protein (GFAP), but negative for cytokeratin and epithelial membrane antigen. In primary cultures, the cells derived from the ECT were morphologically similar to neuronal cells and expressed Nanog, GFAP, and MAP2. RT-PCR analysis of the surgical specimen was positive for OCT3/4, Sox2, Nanog, MAP2, and CD105 mRNAs.
  
  
  CONCLUSIONS
  The results of the present study indicate that ECTs originate from the ectomesenchymal cells of the neural crest and are similar in their molecular and biological characteristics to undifferentiated mesenchymal stem cells.
authors: Department of Molecular Oral Medicine and Maxillofacial Surgery, Applied Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan. yyosioka@hiroshima-u.ac.jp
authors: Yukio
authors: Y
authors: Yoshioka
authors: Ikuko
authors: I
authors: Ogawa
authors: Takaaki
authors: T
authors: Tsunematsu
authors: Taishi
authors: T
authors: Sakaue
authors: Sachiko
authors: S
authors: Yamasaki
authors: Yasuto
authors: Y
authors: Fukui
authors: Yasutaka
authors: Y
authors: Hayashido
authors: Shigeaki
authors: S
authors: Toratani
authors: Tetsuji
authors: T
authors: Okamoto
mesh_terms: D000368:Aged; D001706:Biopsy; D003287:Contrast Media; D003937:Diagnosis, Differential; D005260:Female; D019786:Gadolinium DTPA; D006801:Humans; D007150:Immunohistochemistry; D008279:Magnetic Resonance Imaging; D008637:Mesenchymoma / Q000175:diagnosis* / Q000473:pathology / Q000601:surgery; D009372:Neoplasms, Connective Tissue / Q000175:diagnosis* / Q000473:pathology / Q000601:surgery; D014062:Tongue Neoplasms / Q000175:diagnosis* / Q000473:pathology / Q000601:surgery
chemical_list: D003287:Contrast Media; D019786:Gadolinium DTPA