title: Id2 influences differentiation of killer cell lectin-like receptor G1(hi) short-lived CD8+ effector T cells.
abstract: CD8(+) T cells play a crucial role in the clearance of intracellular pathogens through the generation of cytotoxic effector cells that eliminate infected cells and long-lived memory cells that provide enhanced protection against reinfection. We have previously shown that the inhibitor of E protein transcription factors, Id2, is necessary for accumulation of effector and memory CD8(+) T cells during infection. In this study, we show that CD8(+) T cells lacking Id2 did not generate a robust terminally differentiated killer cell lectin-like receptor G1 (KLRG1)(hi) effector population, but displayed a cell-surface phenotype and cytokine profile consistent with memory precursors, raising the question as to whether loss of Id2 impairs the differentiation and/or survival of effector memory cells. We found that deletion of Bim rescued Id2-deficient CD8(+) cell survival during infection. However, the dramatic reduction in KLRG1(hi) cells caused by loss of Id2 remained in the absence of Bim, such that Id2/Bim double-deficient cells form an exclusively KLRG1(lo)CD127(hi) memory precursor population. Thus, we describe a role for Id2 in both the survival and differentiation of normal CD8(+) effector and memory populations.
authors: Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.
authors: Jamie
authors: J
authors: Knell
authors: J Adam
authors: JA
authors: Best
authors: Nicholas A
authors: NA
authors: Lind
authors: Edward
authors: E
authors: Yang
authors: Louise M
authors: LM
authors: D'Cruz
authors: Ananda W
authors: AW
authors: Goldrath
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D002470:Cell Survival / Q000235:genetics / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D007156:Immunologic Memory / Q000235:genetics; D016130:Immunophenotyping; D051797:Inhibitor of Differentiation Protein 2 / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D053699:Interleukin-7 Receptor alpha Subunit / Q000096:biosynthesis; D037181:Lectins, C-Type; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D011971:Receptors, Immunologic / Q000096:biosynthesis*; D013234:Stem Cells / Q000276:immunology / Q000382:microbiology / Q000821:virology; D051020:bcl-X Protein / Q000172:deficiency / Q000235:genetics
chemical_list: C494812:Bcl2l1 protein, mouse; D016207:Cytokines; C497452:Idb2 protein, mouse; D051797:Inhibitor of Differentiation Protein 2; D053699:Interleukin-7 Receptor alpha Subunit; C451496:Klrg1 protein, mouse; D037181:Lectins, C-Type; D011971:Receptors, Immunologic; D051020:bcl-X Protein