title: Detection of high molecular weight proteins by MALDI imaging mass spectrometry.
abstract: MALDI imaging mass spectrometry (IMS) is a unique technology to explore the spatial distribution of biomolecules directly on tissues. It allows the in situ investigation of a large number of small proteins and peptides. Detection of high molecular weight proteins through MALDI IMS still represents an important challenge, as it would allow the direct investigation of the distribution of more proteins involved in biological processes, such as cytokines, enzymes, neuropeptide precursors and receptors. In this work we compare the traditional method performed with sinapinic acid with a comparable protocol using ferulic acid as the matrix. Data show a remarkable increase of signal acquisition in the mass range of 20k to 150k Th. Moreover, we report molecular images of biomolecules above 70k Th, demonstrating the possibility of expanding the application of this technology both in clinical investigations and basic science.
authors: Department of Health Science, University of Milano-Bicocca, Monza, Italy. veronica.mainini@unimib.it
authors: Veronica
authors: V
authors: Mainini
authors: Giorgio
authors: G
authors: Bovo
authors: Clizia
authors: C
authors: Chinello
authors: Erica
authors: E
authors: Gianazza
authors: Marco
authors: M
authors: Grasso
authors: Giorgio
authors: G
authors: Cattoretti
authors: Fulvio
authors: F
authors: Magni
mesh_terms: D003373:Coumaric Acids / Q000737:chemistry; D006801:Humans; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D003373:Coumaric Acids; D011506:Proteins; C073734:sinapinic acid; C004999:ferulic acid