title: Effects of critical medium components on the production of antifungal lipopeptides from Bacillus amyloliquefaciens Q-426 exhibiting excellent biosurfactant properties.
abstract: In this study, influence of three critical parameters nitrogen sources, initial pH and metal ions was discussed in the production of antifungal lipopeptides from Bacillus amyloliquefaciens Q-426. The results revealed that lipopeptide biosynthesis might have relations with the population density of strain Q-426 and some special amino acids. Also, the alkali-resistant strain Q-426 could grow well in the presence of Fe(2+) ions below 0.8 M l(-1) and still maintain the competitive advantage below 0.2 M l(-1). Moreover, lipopeptides exhibited significant inhibitory activities against Curvularia lunata (Walk) Boed even at the extreme conditions of temperature, pH and salinity. Finally, biosurfactant properties of lipopeptides mixture were evaluated by use with totally six different methods including bacterial adhesion to hydrocarbons assay, lipase activity, hemolytic activity, emulsification activity, oil displacement test and surface tension measurement. The research suggested that B. amyloliquefaciens Q-426 may have great potential in agricultural and environmental fields.
authors: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhong-shan Road, Dalian 116023, China. pengchao_zpc@163.com
authors: Pengchao
authors: P
authors: Zhao
authors: Chunshan
authors: C
authors: Quan
authors: Liming
authors: L
authors: Jin
authors: Lina
authors: L
authors: Wang
authors: Jianhua
authors: J
authors: Wang
authors: Shengdi
authors: S
authors: Fan
mesh_terms: D000935:Antifungal Agents / Q000378:metabolism* / Q000494:pharmacology; D001203:Ascomycota / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D001407:Bacillus / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D006838:Hydrocarbons / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D055666:Lipopeptides / Q000096:biosynthesis* / Q000494:pharmacology; D009584:Nitrogen / Q000378:metabolism; D013500:Surface Tension; D013501:Surface-Active Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D013696:Temperature
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; D006838:Hydrocarbons; D055666:Lipopeptides; D013501:Surface-Active Agents; D009584:Nitrogen