title: Reduced urinary corin levels in patients with chronic kidney disease.
abstract: Corin is a cardiac protease that regulates BP (blood pressure) by activating natriuretic peptides. Recent animal studies identified corin expression in the kidney where it may regulate renal function. In the present study, we tested the hypothesis that corin may be present in human urine and that urinary corin levels may be altered in patients with kidney disease. We obtained urine and kidney tissue samples from normal individuals and CKD (chronic kidney disease) patients. Using ELISA, we detected corin protein in human urine. In normal individuals, urinary corin levels did not correlate with that of plasma, indicating that urinary corin is probably of kidney origin. Compared with normal controls, CKD patients had markedly reduced urinary corin levels and this reduction correlated with disease severity. By immunostaining, human corin protein was identified on the epithelial cell surface in renal tubules. The renal corin mRNA and protein levels were significantly lower in CKD patients than non-CKD controls. The results indicate that renal tubular corin may be shed into urine and that urinary and renal corin levels were reduced in CKD patients. These data suggest that reduced corin levels in the kidney may reflect the underlying pathology in CKD.
authors: The Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, the First Affiliated Hospital, Soochow University, Suzhou 215123, People's Republic of China.
authors: Chaodong
authors: C
authors: Fang
authors: Lei
authors: L
authors: Shen
authors: Liang
authors: L
authors: Dong
authors: Meng
authors: M
authors: Liu
authors: Sensen
authors: S
authors: Shi
authors: Ningzheng
authors: N
authors: Dong
authors: Qingyu
authors: Q
authors: Wu
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood / Q000652:urine; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007684:Kidney Tubules / Q000201:enzymology*; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D051436:Renal Insufficiency, Chronic / Q000097:blood / Q000201:enzymology* / Q000235:genetics / Q000652:urine*; D012697:Serine Endopeptidases / Q000097:blood / Q000235:genetics / Q000652:urine*
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; C119812:CORIN protein, human; D012697:Serine Endopeptidases