title: Vaspin attenuates RANKL-induced osteoclast formation in RAW264.7 cells.
abstract: Visceral adipose tissue-derived serine protease inhibitor (vaspin), an adipokine that was recently identified in a rat model of type 2 diabetes, has been suggested to have an insulin-sensitizing effect. In this study, we investigated whether vaspin inhibits receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis using two types of osteoclast precursors: RAW264.7 cells and bone marrow cells (BMCs). Vaspin inhibited RANKL-induced osteoclastogenesis in RAW264.7 cells and BMCs. Interestingly, vaspin also inhibited the RANKL-induced expression of nuclear factor of activated T cells c1 (NFATc1) in RAW264.7 cells and BMCs. Furthermore, it inhibited the RANKL-induced upregulation of matrix metalloproteinase-9 and cathepsin K in RAW264.7 cells. Thus, we suggest that vaspin downregulates osteoclastogenesis in part by inhibiting expression of the transcription factor NFATc1.
authors: Department of Microbiology, Nihon University School of Dentistry, Tokyo, Japan. kamio.noriaki@nihon-u.ac.jp
authors: Noriaki
authors: N
authors: Kamio
authors: Takayuki
authors: T
authors: Kawato
authors: Natsuko
authors: N
authors: Tanabe
authors: Satoshi
authors: S
authors: Kitami
authors: Toyoko
authors: T
authors: Morita
authors: Kuniyasu
authors: K
authors: Ochiai
authors: Masao
authors: M
authors: Maeno
mesh_terms: D054392:Adipokines / Q000378:metabolism*; D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism; D056657:Cathepsin K / Q000378:metabolism; D002460:Cell Line; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D050778:NFATC Transcription Factors / Q000378:metabolism; D010010:Osteoclasts / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D053245:RANK Ligand / Q000494:pharmacology*; D051381:Rats; D015843:Serpins / Q000378:metabolism*
chemical_list: D054392:Adipokines; D050778:NFATC Transcription Factors; D053245:RANK Ligand; D015843:Serpins; C577327:vaspin protein, mouse; D056657:Cathepsin K; D020780:Matrix Metalloproteinase 9