title: Drosophila splicing factor SF2 knock-down mutant shows altered cell-cycle in vivo.
abstract: Pioneering single gene study documents pre-mRNA processing proteins participation in the cell-cycle regulation of multi-cellular animals (Andersen and Tapon, 2008, J Biol Chem 283: 31256-67). Whole-genome RNAi screen in Drosophila tissue-culture cell lines demonstrates that 17 genes involved in RNA-processing are required for G2/M check-point function (Kondo and Perrimon, 2011, Sci Signal 4: rs1). In particular, the silencing of Splicing Factor 2 (SF2) increases the number of G2(M) cells. We have measured the absolute duration of cell-cycle phases in SF2 depleted flies with the use of flow cytometry and growth parameters of GFP marked mosaic clones. For SF2 mutant cells, G1 = 1.89 h, G2(M) = 7.22 h and S = 1.30 h compared with G1 = 2.25 h, G2(M) = 4.86 h and S = 1.28 h for control normal cells. Thus, G2(M) phase appears to be longer in SF2 silenced cells, supporting the evidence that this splicing protein participates in G2-M check-point function.
authors: Institute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentieva 10, Novosibirsk 630090, Russia.
authors: Tatiana D
authors: TD
authors: Dubatolova
authors: Elena I
authors: EI
authors: Volkova
authors: Leonid V
authors: LV
authors: Omelyanchuk
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002455:Cell Division / Q000235:genetics; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D016195:G2 Phase / Q000235:genetics; D020868:Gene Silencing; D009154:Mutation; D034622:RNA Interference; D000072260:RNA Splicing Factors; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D000072260:RNA Splicing Factors; D016601:RNA-Binding Proteins; C519409:SF2 protein, Drosophila