title: Low potential virulence associated with mutations in the inlA and prfA genes in Listeria monocytogenes isolated from raw retail poultry meat.
abstract: Packaged raw foods can represent a potential source of Listeria monocytogenes contamination when opened at home, and listeriosis is associated with the consumption of undercooked raw foods. The aim of this study was to characterize a group of L. monocytogenes strains isolated  from 56 packages of raw chicken meat from a single brand in order to determine the diversity of the strains that dominate in a particular food over time, as well as their pathogenic potential. Forty (71%) samples were found to be positive for L. monocytogenes, and three isolates per  sample were subjected to PCR molecular serotyping. Subtyping of 45 isolates from different manufacturing dates (n = 40) or different molecular serotype within the same sample (n = 5) identified 11 different L. monocytogenes subtypes as defined by pulsed-field gel electrophoresis  and sequencing of virulence genes actA and inlA. Two of the subtypes accounted for 51% of the isolates. About 40% of isolates (three subtypes) were found to potentially present attenuated virulence because of the presence of mutations in the prfA and inlA genes.
authors: Departamento de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Autopista A-6 km 7.5, 28040 Madrid, Spain.
authors: Victoria
authors: V
authors: López
authors: Jaime
authors: J
authors: Navas
authors: Joaquín V
authors: JV
authors: Martínez-Suárez
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002645:Chickens / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D005838:Genotype; D006801:Humans; D008089:Listeria monocytogenes / Q000235:genetics* / Q000472:pathogenicity*; D008088:Listeriosis / Q000382:microbiology; D008460:Meat / Q000382:microbiology*; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D012703:Serotyping; D037521:Virulence Factors / Q000235:genetics
chemical_list: D037521:Virulence Factors