title: Shrinking hydrogel-DNA spots generates 3D microdots arrays.
abstract: This report describes a straightforward approach for the achievement of sub-100 micrometers size hydrogel dots supporting DNA immobilization. Hydrogel-DNA spots are arrayed and UV-crosslinked on PolyShrink, an innovative polymer material having the remarkable property of isotropically shrinking under high temperature. Curing the microarray enables then spot miniaturization, resulting in 6 µm thick and 60 µm wide hydrogel dots in which oligonucleotides are immobilized in a 3D hydrophilic environment. The probe immobilization within the hydrogel network and its capacity to detect targets specifically and quantitatively is demonstrated using chemiluminescent as well as colorimetric detection techniques. The hydrogel material improves probe accessibility within the spot, leading to an enhanced sensitivity.
authors: Equipe Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, UMR5246 Université Lyon 1-CNRS, Villeurbanne, France.
authors: Gaelle C
authors: GC
authors: Le Goff
authors: Loïc J
authors: LJ
authors: Blum
authors: Christophe A
authors: CA
authors: Marquette
mesh_terms: D020100:Hydrogels / Q000737:chemistry*; D008163:Luminescent Measurements; D008904:Miniaturization; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry; D011108:Polymers; D013696:Temperature; D014466:Ultraviolet Rays
chemical_list: D020100:Hydrogels; D009841:Oligonucleotides; D011108:Polymers