title: miR-34a inhibits the metastasis of osteosarcoma cells by repressing             the expression of CD44.
abstract: Osteosarcoma is the most common type of malignant bone tumor in children             and adolescents and approximately 30% of patients develop lung metastasis, which             is the leading cause of mortality. In this study, we investigated the role of             miR-34a in the invasion and metastasis of osteosarcoma cells by examining its             expression level and functional pattern in these cells. miR-34a mimics were transfected             into the highly metastatic subline, F5M2, and into the F4 subline with low metastatic             potential of the paired human osteosarcoma cell line, SOSP‑9607. Cell viability             patterns, cell migration and alterations in gene expression levels were assessed             by real-time PCR, and changes in protein levels were assessed by immunocytochemistry             and western blot analysis. The ectopic overexpression of miR-34a significantly             inhibited the migration and invasive ability of osteosarcoma cells by repressing             the expression of CD44. These data suggest that miR-34a plays a tumor suppressor             role in the metastasis of osteosarcoma cells by repressing the expression of CD44.             Of note, studies have also suggested that the CD44 protein correlates with the             metastatic potential of several malignant tumors. Therefore, it can be concluded             that through the inhibition of CD44 expression levels, miR-34a plays a significant             role in the migration and invasion of osteosarcoma cells.
authors: Department of Orthopaedic Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710038, PR China.
authors: Haien
authors: H
authors: Zhao
authors: Baoan
authors: B
authors: Ma
authors: Yucai
authors: Y
authors: Wang
authors: Tao
authors: T
authors: Han
authors: Lianhe
authors: L
authors: Zheng
authors: Cong
authors: C
authors: Sun
authors: Tao
authors: T
authors: Liu
authors: Yinglong
authors: Y
authors: Zhang
authors: Xiuchun
authors: X
authors: Qiu
authors: Qingyu
authors: Q
authors: Fan
mesh_terms: D017209:Apoptosis; D001859:Bone Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics* / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism* / Q000556:secondary; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D012516:Osteosarcoma / Q000378:metabolism* / Q000556:secondary; D034622:RNA Interference
chemical_list: C497483:CD44 protein, human; D018960:Hyaluronan Receptors; C522425:MIRN34 microRNA, human; D035683:MicroRNAs