title: Patient-specific orthotopic glioblastoma xenograft models recapitulate the histopathology and biology of human glioblastomas in situ.
abstract: Frequent discrepancies between preclinical and clinical results of anticancer agents demand a reliable translational platform that can precisely recapitulate the biology of human cancers. Another critical unmet need is the ability to predict therapeutic responses for individual patients. Toward this goal, we have established a library of orthotopic glioblastoma (GBM) xenograft models using surgical samples of GBM patients. These patient-specific GBM xenograft tumors recapitulate histopathological properties and maintain genomic characteristics of parental GBMs in situ. Furthermore, in vivo irradiation, chemotherapy, and targeted therapy of these xenograft tumors mimic the treatment response of parental GBMs. We also found that establishment of orthotopic xenograft models portends poor prognosis of GBM patients and identified the gene signatures and pathways signatures associated with the clinical aggressiveness of GBMs. Together, the patient-specific orthotopic GBM xenograft library represent the preclinically and clinically valuable "patient tumor's phenocopy" that represents molecular and functional heterogeneity of GBMs.
authors: Department of Anatomy and Cell Biology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, 135-710, Korea.
authors: Kyeung Min
authors: KM
authors: Joo
authors: Jinkuk
authors: J
authors: Kim
authors: Juyoun
authors: J
authors: Jin
authors: Misuk
authors: M
authors: Kim
authors: Ho Jun
authors: HJ
authors: Seol
authors: Johongir
authors: J
authors: Muradov
authors: Heekyoung
authors: H
authors: Yang
authors: Yoon-La
authors: YL
authors: Choi
authors: Woong-Yang
authors: WY
authors: Park
authors: Doo-Sik
authors: DS
authors: Kong
authors: Jung-Il
authors: JI
authors: Lee
authors: Young-Hyeh
authors: YH
authors: Ko
authors: Hyun Goo
authors: HG
authors: Woo
authors: Jeongwu
authors: J
authors: Lee
authors: Sunghoon
authors: S
authors: Kim
authors: Do-Hyun
authors: DH
authors: Nam
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D001932:Brain Neoplasms / Q000188:drug therapy / Q000473:pathology* / Q000532:radiotherapy / Q000601:surgery; D048430:Cell Shape; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D005260:Female; D015894:Genome, Human / Q000235:genetics; D005909:Glioblastoma / Q000188:drug therapy / Q000473:pathology* / Q000532:radiotherapy / Q000601:surgery; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D051379:Mice; D008875:Middle Aged; D058990:Molecular Targeted Therapy; D009361:Neoplasm Invasiveness; D018874:Spheroids, Cellular / Q000473:pathology; D016896:Treatment Outcome; D023041:Xenograft Model Antitumor Assays; D055815:Young Adult