title: Synthesis of NBD-labeled DOTAP analog to track intracellular delivery of liposome.
abstract: A DOTAP analog labeled by NBD on the head group (DTNBD) was designed and synthesized to label DOTAP liposome. The structure was confirmed by (1)H NMR and FAB-MS, and the fluorescence of the newly synthesized DT-NBD was observed by fluorescent microscopy. The transfection efficiency of DOTAP liposome containing DT-NBD was comparable to commonly used NBD PE in COS7 and MCF7 cells. Furthermore, the level of cellular uptake and fluorescent intensity of fluorescent liposome containing DT-NBD was higher than NBD PE. Therefore, the novel NBD-labeled DOTAP analog seems to be effectively used for investigation of the cellular interaction and transfection mechanism of DOTAP liposome.
authors: Department of Physiology, College of Medicine, Yeungnam University, Daegu 705-717, Korea.
authors: Kyung-Oh
authors: KO
authors: Doh
authors: Bieong-Kil
authors: BK
authors: Kim
authors: Tae-Jin
authors: TJ
authors: Lee
authors: Jong-Won
authors: JW
authors: Park
authors: Young-Bae
authors: YB
authors: Seu
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D004705:Endocytosis; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D061986:MCF-7 Cells; D000644:Quaternary Ammonium Compounds / Q000737:chemistry / Q000378:metabolism*; D013194:Staining and Labeling
chemical_list: D005229:Fatty Acids, Monounsaturated; D005456:Fluorescent Dyes; D008081:Liposomes; D000644:Quaternary Ammonium Compounds; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane