title: Benzophenone-1 stimulated the growth of BG-1 ovarian cancer cells by cell cycle regulation via an estrogen receptor alpha-mediated signaling pathway in cellular and xenograft mouse models.
abstract: 2,4-Dihydroxybenzophenone (benzophenone-1; BP-1) is an UV stabilizer primarily used to prevent polymer degradation and deterioration in quality due to UV irradiation. Recently, BP-1 has been reported to bioaccumulate in human bodies by absorption through the skin and has the potential to induce health problems including endocrine disruption. In the present study, we examined the xenoestrogenic effect of BP-1 on BG-1 human ovarian cancer cells expressing estrogen receptors (ERs) and relevant xenografted animal models in comparison with 17-β estradiol (E2). In in vitro cell viability assay, BP-1 (10(-8)-10(-5)M) significantly increased BG-1 cell growth the way E2 did. The mechanism underlying the BG-1 cell proliferation was proved to be related with the up-regulation of cyclin D1, a cell cycle progressor, by E2 or BP-1. Both BP-1 and E2 induced cell growth and up-regulation of cyclin D1 were reversed by co-treatment with ICI 182,780, an ER antagonist, suggesting that BP-1 may mediate the cancer cell proliferation via an ER-dependent pathway like E2. On the other hand, the expression of p21, a regulator of cell cycle progression at G1 phase, was not altered by BP-1 though it was down-regulated by E2. In xenograft mouse models transplanted with BG-1 cells, BP-1 or E2 treatment significantly increased the tumor mass formation compared to a vehicle (corn oil) within 8 weeks. In histopathological analysis, the tumor sections of E2 or BP-1 group displayed extensive cell formations with high density and disordered arrangement, which were supported by the increased number of BrdUrd positive nuclei and the over-expression of cyclin D1 protein. Taken together, these results suggest that BP-1 is an endocrine disrupting chemical (EDC) that exerts xenoestrogenic effects by stimulating the proliferation of BG-1 ovarian cancer via ER signaling pathway associated with cell cycle as did E2.
authors: Laboratory of Veterinary Biochemistry and Immunology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361-763 Republic of Korea.
authors: Min-Ah
authors: MA
authors: Park
authors: Kyung-A
authors: KA
authors: Hwang
authors: Hye-Rim
authors: HR
authors: Lee
authors: Bo-Rim
authors: BR
authors: Yi
authors: Eui-Bae
authors: EB
authors: Jeung
authors: Kyung-Chul
authors: KC
authors: Choi
mesh_terms: D000818:Animals; D000963:Antimetabolites; D001577:Benzophenones / Q000633:toxicity*; D015153:Blotting, Western; D001973:Bromodeoxyuridine; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004396:Coloring Agents; D019938:Cyclin D1 / Q000378:metabolism; D047628:Estrogen Receptor alpha / Q000819:agonists / Q000037:antagonists & inhibitors / Q000187:drug effects*; D005260:Female; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D010051:Ovarian Neoplasms / Q000139:chemically induced* / Q000473:pathology*; D011247:Pregnancy; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects*; D013268:Stimulation, Chemical; D023041:Xenograft Model Antitumor Assays; D054462:p21-Activated Kinases / Q000378:metabolism
chemical_list: D000963:Antimetabolites; D001577:Benzophenones; D004396:Coloring Agents; D047628:Estrogen Receptor alpha; D012334:RNA, Neoplasm; D019938:Cyclin D1; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D054462:p21-Activated Kinases; D001973:Bromodeoxyuridine; C121479:2,4-dihydroxybenzophenone