title: Genetic diversity among multidrug-resistant Mycobacterium tuberculosis strains in Mexico.
abstract: Tuberculosis is an important public health problem in Mexico. However, limited information about the genetic diversity of Mycobacterium tuberculosis strains circulating in the country is available. In this work, 109 multidrug-resistant (MDR) M. tuberculosis isolates collected in 23 different states of Mexico in 2003 were retrospectively characterized by spoligotyping and MIRU-VNTRs. All isolates, except for a single cluster containing two strains (subcluster E1), were split when information from the 12-loci MIRUs and spoligo-pattern was simultaneously analyzed. The discriminative power of 12-loci MIRU-VNTR and spoligotyping, by the Hunter-Gaston index, were 0.9998 and 0.9011, respectively. These findings suggest that almost all cases were epidemiologically unrelated. Instead, the genetic variations observed among these strains are suggestive of emergence of acquired drug-resistance during the course of treatment. The results suggest a high degree of genetic variability and a high frequency of SIT53 (T1 family) spoligotype among the MDR M. tuberculosis isolates included in the study.
authors: Instituto de Diagnóstico y Referencia Epidemiológicos, Secretaría de Salud, Mexico City, Mexico.
authors: Armando
authors: A
authors: Martinez-Guarneros
authors: Nalin
authors: N
authors: Rastogi
authors: David
authors: D
authors: Couvin
authors: Alejandro
authors: A
authors: Escobar-Gutierrez
authors: Livia Maria Gonçalves
authors: LM
authors: Rossi
authors: Carlos A
authors: CA
authors: Vazquez-Chacon
authors: Sandra
authors: S
authors: Rivera-Gutierrez
authors: Daniela
authors: D
authors: Lozano
authors: Arely
authors: A
authors: Vergara-Castañeda
authors: Jorge A
authors: JA
authors: Gonzalez-Y-Merchand
authors: Gilberto
authors: G
authors: Vaughan
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000904:Antibiotics, Antitubercular / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D004269:DNA, Bacterial; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D008800:Mexico / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D018598:Minisatellite Repeats; D058889:Molecular Typing; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D019371:Topography, Medical; D018088:Tuberculosis, Multidrug-Resistant / Q000453:epidemiology*; D055815:Young Adult
chemical_list: D000904:Antibiotics, Antitubercular; D004269:DNA, Bacterial