title: The interaction of 2-mercaptobenzimidazole with human serum albumin as determined by spectroscopy, atomic force microscopy and molecular modeling.
abstract: The interaction of 2-mercaptobenzimidazole (MBI) with human serum albumin (HSA) was studied in vitro by equilibrium dialysis under normal physiological conditions. This study used fluorescence, ultraviolet-visible spectroscopy (UV-vis), Fourier transform infrared (FT-IR), circular dichroism (CD) and Raman spectroscopy, atomic force microscopy (AFM) and molecular modeling techniques. Association constants, the number of binding sites and basic thermodynamic parameters were used to investigate the quenching mechanism. Based on the fluorescence resonance energy transfer, the distance between the HSA and MBI was 2.495 nm. The ΔG(0), ΔH(0), and ΔS(0) values across temperature indicated that the hydrophobic interaction was the predominant binding Force. The UV, FT-IR, CD and Raman spectra confirmed that the HSA secondary structure was altered in the presence of MBI. In addition, the molecular modeling showed that the MBI-HSA complex was stabilized by hydrophobic forces, which resulted from amino acid residues. The AFM results revealed that the individual HSA molecule dimensions were larger after interaction with MBI. Overall, this study suggested a method for characterizing the weak intermolecular interaction. In addition, this method is potentially useful for elucidating the toxigenicity of MBI when it is combined with the biomolecular function effect, transmembrane transport, toxicological testing and other experiments.
authors: Department of Pharmacy, Taishan Medicine College, Taian, Shandong 271016, China.
authors: Yuqin
authors: Y
authors: Li
authors: Baoxiu
authors: B
authors: Jia
authors: Hao
authors: H
authors: Wang
authors: Nana
authors: N
authors: Li
authors: Gaopan
authors: G
authors: Chen
authors: Yuejuan
authors: Y
authors: Lin
authors: Wenhua
authors: W
authors: Gao
mesh_terms: D001562:Benzimidazoles / Q000737:chemistry*; D002942:Circular Dichroism; D006801:Humans; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman
chemical_list: D001562:Benzimidazoles; D012709:Serum Albumin; C030698:2-mercaptobenzimidazole