title: Gene expression changes in adipose tissue with diet- and/or exercise-induced weight loss.
abstract: Adipose tissue plays a role in obesity-related cancers via increased production of inflammatory factors, steroid hormones, and altered adipokines. The impact of weight loss on adipose tissue gene expression may provide insights into pathways linking obesity with cancer risk. We conducted an ancillary study within a randomized trial of diet, exercise, or combined diet + exercise versus control among overweight/obese postmenopausal women. In 45 women, subcutaneous adipose tissue biopsies were conducted at baseline and after 6 months, and changes in adipose tissue gene expression were determined by microarray with an emphasis on prespecified candidate pathways as well as by unsupervised clustering of more than 37,000 transcripts (Illumina). Analyses were conducted first by randomization group and then by degree of weight change at 6-months in all women combined. At 6 months, diet, exercise, and diet + exercise participants lost a mean of 8.8, 2.5, and 7.9 kg (all P < 0.05 vs. no change in controls). There was no significant change in candidate gene expression by intervention group. In analysis by weight change category, greater weight loss was associated a decrease in 17β-hydroxysteroid dehydrogenase-1 (HSD17B1, Ptrend < 0.01) and leptin (LEP, Ptrend < 0.01) expression, and marginally significant increased expression of estrogen receptor-1 (ESR1, Ptrend = 0.08) and insulin-like growth factor-binding protein-3 (IGFBP3, Ptrend = 0.08). Unsupervised clustering revealed 83 transcripts with statistically significant changes. Multiple gene expression changes correlated with changes in associated serum biomarkers. Weight loss was associated with changes in adipose tissue gene expression after 6 months, particularly in two pathways postulated to link obesity and cancer, that is, steroid hormone metabolism and IGF signaling. Cancer Prev Res; 6(3); 217-31. ©2013 AACR.
authors: National Center for Tumor Diseases (NCT), Division of Preventive Oncology, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 460, Heidelberg 69120, Germany. neli.ulrich@nct-heidelberg.de
authors: Kristin L
authors: KL
authors: Campbell
authors: Karen E
authors: KE
authors: Foster-Schubert
authors: Karen W
authors: KW
authors: Makar
authors: Mario
authors: M
authors: Kratz
authors: Derek
authors: D
authors: Hagman
authors: Ellen A
authors: EA
authors: Schur
authors: Nina
authors: N
authors: Habermann
authors: Marc
authors: M
authors: Horton
authors: Clare
authors: C
authors: Abbenhardt
authors: Ling-Yu
authors: LY
authors: Kuan
authors: Liren
authors: L
authors: Xiao
authors: Jerry
authors: J
authors: Davison
authors: Martin
authors: M
authors: Morgan
authors: Ching-Yun
authors: CY
authors: Wang
authors: Catherine
authors: C
authors: Duggan
authors: Anne
authors: A
authors: McTiernan
authors: Cornelia M
authors: CM
authors: Ulrich
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000368:Aged; D016000:Cluster Analysis; D004038:Diet, Reducing; D015444:Exercise; D005260:Female; D006801:Humans; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome; D015431:Weight Loss / Q000235:genetics*; D061217:Weight Reduction Programs