title: The Lotus japonicus MAMI gene links root development, arbuscular mycorrhizal symbiosis and phosphate availability.
abstract: The arbuscular mycorrhizal-induced LjMAMI gene is phylogenetically related to GARP transcription factors involved in Pi-starvation responses such as AtPHR1. The gene is strongly upregulated in arbusculated cells from mycorrhizal plants and in root meristems, irrespectively of the fungal presence. A further expression analysis revealed a similar expression pattern for LjPT4, considered a marker gene for mycorrhizal functionality. Here we show that the LjPT4 promoter contains two conserved cis-acting elements typically found in Pi-starvation induced Pi transporters. One of these is strongly related to the binding site of AtPHR1, suggesting a direct regulation of LjPT4 by LjMAMI. The expression of both genes in non-mycorrhizal tissues leads to the hypothesis that these symbiosis-inducible genes are also involved in Pi starvation responses in root meristems in an AM-independent manner.
authors: Department of Life Science and Systems Biology; University of Torino; Torino, Italy.
authors: Veronica
authors: V
authors: Volpe
authors: Elisa
authors: E
authors: Dell'Aglio
authors: Paola
authors: P
authors: Bonfante
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D000070116:Lotus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D018519:Meristem / Q000254:growth & development / Q000378:metabolism; D038821:Mycorrhizae / Q000235:genetics*; D028061:Phosphate Transport Proteins / Q000235:genetics* / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D013559:Symbiosis; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D029681:Arabidopsis Proteins; C504848:Lux Arrhythmo protein, Arabidopsis; C514695:PHR1 protein, Arabidopsis; D028061:Phosphate Transport Proteins; D010710:Phosphates; D010940:Plant Proteins; D014157:Transcription Factors
keywords: phosphate transporter; putative-transcription factor; root architecture; symbiosis