title: The role of xenobotic metabolism MGST1 gene polymorphism in colorectal cancer patients.
abstract: OBJECTIVE
  to asses the role of Microsomal Glutathione S-Transferase1 (MGST1) gene as one of enzym metabolism that plays in enviromental factor.
  
  
  METHODS
  using case-control study, subjects with age less than 50 years were collected from teaching hospital Makassar between 2008-2010. Frozen or routinely processed tumour samples biopsy and peripheral blood were obtained from 35 CRC patients undergoing surgery and endoscopic examination with 61 subject as control. CRC cases were diagnosis by clinical examination and confirm by histopathology without familial aggregation of CRC. DNA resequencing was conducted for the 3 kb genomic DNA region MGST1 using PCR-restriction fragment length polymorphism (PCR-RFLP).
  
  
  RESULTS
  from 96 subject, two varian single nucleotide polymorphisms (SNPs) 16454T>G and 16416G>A MGST1 were identified. Significant CRC association (p= 0.047) was detected in GG genotipe SNP 16454T>G MGST1 with 3.5 fold risk (95% confidence interval (CI) 0.962-13.191).
  
  
  CONCLUSIONS
  the results suggest that MGST1 gene polymorphisms as one of environment gene may contribute to CRC risk in younger age (<50 years old).
authors: Centre of Gastroentero-Hepatology, Department of Internal Medicine, Faculty of Medicine, Hasanuddin University-Wahidin Sudirohusodo Hospital. Jl. Perintis Kemerdekaan KM 11, Makassar, Indonesia. dndakil@gmail.com
authors: Fardah
authors: F
authors: Akil
authors: H A M
authors: HA
authors: Akil
authors: A M
authors: AM
authors: Lutfie
authors: Wahyu S
authors: WS
authors: Wibowo
authors: Upik
authors: U
authors: Miskad
authors: Irawan
authors: I
authors: Yusuf
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D007214:Indonesia; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
chemical_list: C122107:microsomal glutathione S-transferase-I; D005982:Glutathione Transferase