title: Apolipoprotein A-IV as a novel gene associated with polycystic ovary             syndrome.
abstract: Polycystic ovary syndrome (PCOS) is a common endocrine-metabolic disorder,             affecting 6-10% of women of reproductive age. The etiology remains poorly understood.             To investigate the differentially expressed proteins from PCOS patients versus             healthy women, the protein expression in follicular fluid was analyzed using two-dimensional             electrophoresis (2-DE). Since follicular fluid contains a number of secretory             proteins required for oocyte fertilization and follicle maturation, it is possible             that follicular fluid can be used as a provisional source for identifying pivotal             proteins associated with PCOS. In this study, six overexpressed proteins [kininogen 1,             cytokeratin 9, antithrombin, fibrinogen γ-chain, apolipoprotein A-IV (apoA-IV)             precursor and α-1-B-glycoprotein (A1BG)] in follicular fluids from PCOS patients             were identified with matrix-assisted laser desorption/ionization-time-of-flight             mass spectrometry (MALDI-TOF-MS) and nano LC-MS/MS. Western blot analysis confirmed             that the protein expression levels of apoA-IV precursor and A1BG were increased             in follicular fluid from PCOS patients compared with those from normal controls.             The analysis of protein expression for other proteins revealed individual variation.             These results facilitate the understanding of the molecular mechanisms of PCOS             and provide candidate biomarkers for the development of diagnostic and therapeutic             tools.
authors: Department of Biomedical Science, Fertility Center, CHA University,                     CHA General Hospital, Seoul, Republic of Korea.
authors: Yong-Soo
authors: YS
authors: Kim
authors: Bon-Hee
authors: BH
authors: Gu
authors: Bum-Chae
authors: BC
authors: Choi
authors: Myung-Sun
authors: MS
authors: Kim
authors: Sangjin
authors: S
authors: Song
authors: Ji-Hyun
authors: JH
authors: Yun
authors: Mi Kyung
authors: MK
authors: Chung
authors: Cheol-Hee
authors: CH
authors: Choi
authors: Kwang-Hyun
authors: KH
authors: Baek
mesh_terms: D000328:Adult; D000991:Antithrombins / Q000032:analysis; D001054:Apolipoproteins A / Q000096:biosynthesis* / Q000235:genetics*; D005260:Female; D015241:Fibrinogens, Abnormal / Q000096:biosynthesis; D015571:Follicular Fluid / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis / Q000235:genetics; D053534:Keratin-9 / Q000032:analysis / Q000096:biosynthesis; D007704:Kininogens / Q000096:biosynthesis; D006080:Ovarian Follicle / Q000378:metabolism*; D011085:Polycystic Ovary Syndrome / Q000235:genetics* / Q000378:metabolism*; D040901:Proteomics
chemical_list: C048566:A1BG protein, human; D000991:Antithrombins; D001054:Apolipoproteins A; D015241:Fibrinogens, Abnormal; D006023:Glycoproteins; D007136:Immunoglobulins; D053534:Keratin-9; D007704:Kininogens; C022205:apolipoprotein A-IV; C527521:fibrinogen gamma'