title: Functional interplay between the cell cycle and cell phenotypes.
abstract: Cell cycle distribution of adherent cells is typically assessed using flow cytometry, which precludes the measurements of many cell properties and their cycle phase in the same environment. Here we develop and validate a microscopy system to quantitatively analyze the cell-cycle phase of thousands of adherent cells and their associated cell properties simultaneously. This assay demonstrates that population-averaged cell phenotypes can be written as a linear combination of cell-cycle fractions and phase-dependent phenotypes. By perturbing the cell cycle through inhibition of cell-cycle regulators or changing nuclear morphology by depletion of structural proteins, our results reveal that cell cycle regulators and structural proteins can significantly interfere with each other's prima facie functions. This study introduces a high-throughput method to simultaneously measure the cell cycle and phenotypes at single-cell resolution, which reveals a complex functional interplay between the cell cycle and cell phenotypes.
authors: Johns Hopkins Physical Sciences-Oncology Center, The Johns Hopkins University, Baltimore, Maryland 21218, USA.
authors: Wei-Chiang
authors: WC
authors: Chen
authors: Pei-Hsun
authors: PH
authors: Wu
authors: Jude M
authors: JM
authors: Phillip
authors: Shyam B
authors: SB
authors: Khatau
authors: Jae Min
authors: JM
authors: Choi
authors: Matthew R
authors: MR
authors: Dallas
authors: Konstantinos
authors: K
authors: Konstantopoulos
authors: Sean X
authors: SX
authors: Sun
authors: Jerry S H
authors: JS
authors: Lee
authors: Didier
authors: D
authors: Hodzic
authors: Denis
authors: D
authors: Wirtz
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D002453:Cell Cycle; D002460:Cell Line; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D051358:Cyclin-Dependent Kinase 4 / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D034904:Lamin Type A / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D008954:Models, Biological; D010641:Phenotype
chemical_list: D000199:Actins; D034904:Lamin Type A; D004247:DNA; C495900:CDK4 protein, human; D051358:Cyclin-Dependent Kinase 4