title: Genetic variation in the endocannabinoid degrading enzyme fatty acid amide hydrolase (FAAH) and their influence on weight loss and insulin resistance under a high monounsaturated fat hypocaloric diet.
abstract: OBJECTIVE
  The C385A polymorphism of FAAH gene (rs324420C>A) has been associated with obesity. We investigate the role of this polymorphism on anthropometric and metabolic responses after an enriched monounsaturated fat hypocaloric diet.
  
  
  METHODS
  A sample of 95 obese individuals was analyzed at baseline and after 3 months of an enriched monounsaturated fat hypocaloric diet.
  
  
  RESULTS
  Sixty two patients (65.3%) had the genotype C385C and 33 (34.7%) patients had C385A genotype (30 patients, 31.6%) or A358A (3 patients, 3.2%) (A carriers group). In subjects with C385C genotype, insulin (-1.9±5.3 mUI/l) and HOMA-R (-0.48±0.75 U) decreased. In A carriers subjects, the decreases in weight were 3.7±3.4 kg (decrease in C385C genotype group 4.4±3.6 kg), fat mass 2.7±3.2 kg (decrease in C385C genotype group 3.4±3.2 kg) and waist circumference 3.1±3.4cm (decrease in C385 genotype group 4.4±4.6 cm). These changes were significantly higher in the C385C genotype group than the A carriers subjects.
  
  
  CONCLUSIONS
  After weight loss, noncarriers of the allele A385 of FAAH had an improvement on insulin and HOMA-R levels with an enriched monounsaturated fat hypocaloric diet. A better response of weight, fat mass and waist circumference was observed in C385 genotype subjects than A carriers participants.
authors: Institute of Endocrinology and Nutrition, Medicine School and Unit of Investigation, Hospital Rio Hortega, University of Valladolid, C/Los perales 16, Simancas 47130, Valladolid, Spain. dadluis@yahoo.es
authors: Daniel
authors: D
authors: de Luis
authors: Rocio
authors: R
authors: Aller
authors: Olatz
authors: O
authors: Izaola
authors: Rosa
authors: R
authors: Conde
authors: Beatriz
authors: B
authors: de la Fuente
authors: Manuel
authors: M
authors: Gonzalez Sagrado
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D000581:Amidohydrolases / Q000235:genetics* / Q000378:metabolism; D015992:Body Mass Index; D004038:Diet, Reducing; D005229:Fatty Acids, Monounsaturated / Q000627:therapeutic use*; D005260:Female; D005500:Follow-Up Studies; D056726:Genetic Association Studies; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000178:diet therapy* / Q000235:genetics / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D011446:Prospective Studies; D013030:Spain; D055105:Waist Circumference; D015431:Weight Loss; D000099252:Fatty Acid Amide Hydrolases
chemical_list: D000581:Amidohydrolases; D005229:Fatty Acids, Monounsaturated; D007328:Insulin; D000099252:Fatty Acid Amide Hydrolases