title: Visualizing the location and the dynamics of gene expression in living animals through bioluminescence imaging.
abstract: Bioluminescence imaging allows for real-time assessment of gene expression in vivo in models where luciferase expression is controlled by promoter elements of gene of interest. It provides a sensitive means of recording temporal and spatial resolution of gene expression. In this Chapter, protocols for the use of the bioluminescence imaging system in localizing and semi-quantitatively measuring gene expression in mice are discussed.
authors: Columbia Center for Translational Immunology, Columbia University College of Physicians and Surgeons, New York, NY 10032-3702, USA.
authors: Xiaojuan
authors: X
authors: Chen
mesh_terms: D000818:Animals; D052160:Benzothiazoles / Q000008:administration & dosage / Q000493:pharmacokinetics; D015870:Gene Expression; D007091:Image Processing, Computer-Assisted; D007274:Injections, Intraperitoneal; D007328:Insulin / Q000235:genetics / Q000378:metabolism; D049409:Luciferases, Firefly / Q000096:biosynthesis / Q000235:genetics; D008163:Luminescent Measurements / Q000379:methods; D051379:Mice; D008822:Mice, Transgenic; D061848:Optical Imaging / Q000379:methods*; D051598:Whole Body Imaging / Q000379:methods*
chemical_list: D052160:Benzothiazoles; C532924:D-luciferin; D007328:Insulin; D049409:Luciferases, Firefly