title: Enzyme molecules as nanomotors.
abstract: Using fluorescence correlation spectroscopy, we show that the diffusive movements of catalase enzyme molecules increase in the presence of the substrate, hydrogen peroxide, in a concentration-dependent manner. Employing a microfluidic device to generate a substrate concentration gradient, we show that both catalase and urease enzyme molecules spread toward areas of higher substrate concentration, a form of chemotaxis at the molecular scale. Using glucose oxidase and glucose to generate a hydrogen peroxide gradient, we induce the migration of catalase toward glucose oxidase, thereby showing that chemically interconnected enzymes can be drawn together.
authors: Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
authors: Samudra
authors: S
authors: Sengupta
authors: Krishna K
authors: KK
authors: Dey
authors: Hari S
authors: HS
authors: Muddana
authors: Tristan
authors: T
authors: Tabouillot
authors: Michael E
authors: ME
authors: Ibele
authors: Peter J
authors: PJ
authors: Butler
authors: Ayusman
authors: A
authors: Sen
mesh_terms: D002374:Catalase / Q000737:chemistry* / Q000378:metabolism; D005947:Glucose / Q000737:chemistry* / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry* / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry*; D014510:Urease / Q000737:chemistry* / Q000378:metabolism
chemical_list: D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D002374:Catalase; D014510:Urease; D005947:Glucose