title: Neonatal plasmacytoid dendritic cells (pDCs) display subset variation but can elicit potent anti-viral innate responses.
abstract: Neonates are highly susceptible to infectious diseases and defective antiviral pDC immune responses have been proposed to contribute to this phenomenon. Isolated cord blood pDCs innately responded to a variety of TLR7 and TLR9 dependent viruses, including influenza A virus (IAV), human immunodeficiency virus (HIV) or herpes-simplex virus (HSV) by efficiently producing IFN-α, TNF-α as well as chemokines. Interestingly, following activation by CpGA, but not viruses, cord pDCs tend to survive less efficiently. We found that a hallmark of pDCs in neonates is an extended CD2+pDCs compartment compared to adult pDCs without affecting the antiviral IFN-α response. Within CD2+pDCs, we identified a subpopulation expressing CD5 and responsible for IL-12p40 production, however this population is significantly decreased in cord blood compared to adult blood. Therefore, neonatal pDCs clearly display variation in phenotype and subset composition, but without major consequences for their antiviral responses.
authors: Régulation Immunitaire et Vaccinologie, Institut Pasteur, Paris, France.
authors: Xiaoming
authors: X
authors: Zhang
authors: Alice
authors: A
authors: Lepelley
authors: Elie
authors: E
authors: Azria
authors: Pierre
authors: P
authors: Lebon
authors: Gwenaelle
authors: G
authors: Roguet
authors: Olivier
authors: O
authors: Schwartz
authors: Odile
authors: O
authors: Launay
authors: Claude
authors: C
authors: Leclerc
authors: Richard
authors: R
authors: Lo-Man
mesh_terms: D000293:Adolescent; D000328:Adult; D018801:CD2 Antigens / Q000276:immunology / Q000378:metabolism; D018956:CD5 Antigens / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D018925:Chemokines / Q000276:immunology* / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D005434:Flow Cytometry; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects / Q000276:immunology; D007231:Infant, Newborn; D016898:Interferon-alpha / Q000276:immunology* / Q000378:metabolism; D053762:Interleukin-12 Subunit p40 / Q000276:immunology / Q000378:metabolism; D008875:Middle Aged; D009838:Oligodeoxyribonucleotides / Q000276:immunology / Q000494:pharmacology; D051199:Toll-Like Receptor 7 / Q000276:immunology / Q000378:metabolism; D051217:Toll-Like Receptor 9 / Q000276:immunology / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology* / Q000378:metabolism
chemical_list: D018801:CD2 Antigens; D018956:CD5 Antigens; D018925:Chemokines; D016898:Interferon-alpha; D053762:Interleukin-12 Subunit p40; D009838:Oligodeoxyribonucleotides; C495348:TLR7 protein, human; D051199:Toll-Like Receptor 7; D051217:Toll-Like Receptor 9; D014409:Tumor Necrosis Factor-alpha