title: Effect of an acrylic resin combined with an antimicrobial polymer on biofilm formation.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the antimicrobial activity of an acrylic resin combined with an antimicrobial polymer poly (2-tert-butylaminoethyl) methacrylate (PTBAEMA) to inhibit Staphylococcus aureus, Streptococcus mutans and Candida albicans biofilm formation.
  
  
  METHODS
  Discs of a heat-polymerized acrylic resin were produced and divided according to PTBAEMA concentration: 0 (control), 10 and 25%. The specimens were inoculated (10(7) CFU/mL) and incubated at 37ºC for 48 h. After incubation, the wells were washed and each specimen was sonicated for 20 min. Replicate aliquots of resultant suspensions were plated at dilutions at 37ºC for 48 h. The number of colony-forming units (CFU) was counted and expressed as log (CFU+1)/mL and analyzed statistically with α=.05.
  
  
  RESULTS
  The results showed that 25% PTBAEMA completely inhibited S. aureus and S. mutans biofilm formation. A significant reduction of log (CFU+1)/mL in count of S. aureus (control: 7.9±0.8A; 10%: 3.8±3.3B) and S. mutans (control: 7.5±0.7A; 10%: 5.1±2.7B) was observed for the group containing 10% PTBAEMA (Mann-Whitney, p<0.05). For C. albicans, differences were not significant among the groups (control: 6.6±0.2A; 10%: 6.6±0.4A; 25%: 6.4±0.1A), (Kruskal-Wallis, p>0.05, P=0.079).
  
  
  CONCLUSIONS
  Acrylic resin combined with 10 and 25% of PTBAEMA showed significant antimicrobial activity against S. aureus and S. mutans biofilm, but it was inactive against the C. albicans biofilm.
authors: Department of Prosthodontics, Paulista University, Goiânia, GO, Brazil.
authors: Juliê
authors: J
authors: Marra
authors: André Gustavo
authors: AG
authors: Paleari
authors: Larissa Santana
authors: LS
authors: Rodriguez
authors: Andressa Rosa Perin
authors: AR
authors: Leite
authors: Ana Carolina
authors: AC
authors: Pero
authors: Marco Antonio
authors: MA
authors: Compagnoni
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002176:Candida albicans / Q000254:growth & development*; D015169:Colony Count, Microbial; D003819:Denture Bases / Q000382:microbiology; D008689:Methacrylates / Q000737:chemistry / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000254:growth & development*; D013234:Stem Cells; D013295:Streptococcus mutans / Q000254:growth & development*
chemical_list: D000180:Acrylic Resins; D000890:Anti-Infective Agents; D008689:Methacrylates; C522816:poly(2-(tert-butylamino)ethyl methacrylate)