title: Longitudinal study of [D10]phenanthrene metabolism by the diol epoxide pathway in smokers.
abstract: The extent of metabolism of [D10]phenanthrene to [D(10)]r-1,t-2,3,c-4-tetrahydroxy-1,2,3,4-tetradeuterophenanthrene ([D10]PheT) could be a biomarker of human metabolic activation of carcinogenic polycyclic aromatic hydrocarbons, leading to identification of smokers particularly susceptible to lung cancer. The longitudinal stability of [D10]PheT was evaluated in 24 cigaret smokers given 7-8 oral doses of [D10]phenanthrene (10 µg) over 5.5 months. [D10]PheT in 6 h urine was quantified after each dose. The overall coefficient of variation for 24 subjects was (mean ± S.D.) 27.4% ± 8.83%. Thus, a single administration of [D10]phenanthrene is likely sufficient to determine a smoker's ability to metabolize it to [D10]PheT.
authors: Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA. hecht002@umn.edu
authors: Stephen S
authors: SS
authors: Hecht
authors: J Bradley
authors: JB
authors: Hochalter
authors: Steven G
authors: SG
authors: Carmella
authors: Yan
authors: Y
authors: Zhang
authors: Diane M
authors: DM
authors: Rauch
authors: Naomi
authors: N
authors: Fujioka
authors: Joni
authors: J
authors: Jensen
authors: Dorothy K
authors: DK
authors: Hatsukami
mesh_terms: D000284:Administration, Oral; D000328:Adult; D015415:Biomarkers / Q000652:urine; D001711:Biotransformation; D002273:Carcinogens / Q000378:metabolism* / Q000493:pharmacokinetics; D004198:Disease Susceptibility; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008137:Longitudinal Studies; D008175:Lung Neoplasms / Q000209:etiology / Q000517:prevention & control* / Q000652:urine; D008297:Male; D008875:Middle Aged; D010616:Phenanthrenes / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000652:urine*; D012907:Smoking / Q000009:adverse effects
chemical_list: D015415:Biomarkers; D002273:Carcinogens; D010616:Phenanthrenes; C502234:r-1,t-2,3,c-4-tetrahydroxy-1,2,3,4-tetrahydrophenanthrene; C031181:phenanthrene