title: The Ecm11-Gmc2 complex promotes synaptonemal complex formation through assembly of transverse filaments in budding yeast.
abstract: During meiosis, homologous chromosomes pair at close proximity to form the synaptonemal complex (SC). This association is mediated by transverse filament proteins that hold the axes of homologous chromosomes together along their entire length. Transverse filament proteins are highly aggregative and can form an aberrant aggregate called the polycomplex that is unassociated with chromosomes. Here, we show that the Ecm11-Gmc2 complex is a novel SC component, functioning to facilitate assembly of the yeast transverse filament protein, Zip1. Ecm11 and Gmc2 initially localize to the synapsis initiation sites, then throughout the synapsed regions of paired homologous chromosomes. The absence of either Ecm11 or Gmc2 substantially compromises the chromosomal assembly of Zip1 as well as polycomplex formation, indicating that the complex is required for extensive Zip1 polymerization. We also show that Ecm11 is SUMOylated in a Gmc2-dependent manner. Remarkably, in the unSUMOylatable ecm11 mutant, assembly of chromosomal Zip1 remained compromised while polycomplex formation became frequent. We propose that the Ecm11-Gmc2 complex facilitates the assembly of Zip1 and that SUMOylation of Ecm11 is critical for ensuring chromosomal assembly of Zip1, thus suppressing polycomplex formation.
authors: MRC Genome Damage and Stability Centre, University of Sussex, Brighton, United Kingdom.
authors: Neil
authors: N
authors: Humphryes
authors: Wing-Kit
authors: WK
authors: Leung
authors: Bilge
authors: B
authors: Argunhan
authors: Yaroslav
authors: Y
authors: Terentyev
authors: Martina
authors: M
authors: Dvorackova
authors: Hideo
authors: H
authors: Tsubouchi
mesh_terms: D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002503:Centromere / Q000235:genetics / Q000378:metabolism; D023902:Chromosome Pairing / Q000235:genetics; D015825:Chromosomes, Fungal / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D008540:Meiosis / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D025842:SUMO-1 Protein / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013573:Synaptonemal Complex / Q000235:genetics / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; C529900:Ecm11 protein, S cerevisiae; D005656:Fungal Proteins; D009687:Nuclear Proteins; D025842:SUMO-1 Protein; D029701:Saccharomyces cerevisiae Proteins; C090118:Zip1 protein, S cerevisiae