title: Mapping O-glycosylation of apolipoprotein C-III in MALDI-FT-ICR protein profiles.
abstract: Ultrahigh resolution MALDI-FT-ICR profiles were obtained from human serum samples that were processed using a fully automated RPC18-based magnetic bead method. Proteins were profiled from m/z value 6630 with a resolving power of 73 000 up to m/z value 12 600 with a resolving power of 37 000. In this study, a detailed evaluation was performed of the isoforms of apolipoprotein C-III, i.e. the different mucin-type core 1 O-glycans with the addition of one or two sialic acid residues. The MALDI-FT-ICR profiles are discussed with regard to reproducibility of the signal intensities as well as the accurate mass measurements. ESI-FT-ICR-MS/MS analyses of the same serum samples were performed to confirm the identity of apolipoprotein C-III glycoforms.
authors: Center for Proteomics and Metabolomics, Leiden University Medical Center (LUMC), Leiden, The Netherlands.
authors: Simone
authors: S
authors: Nicolardi
authors: Yuri E M
authors: YE
authors: van der Burgt
authors: Manfred
authors: M
authors: Wuhrer
authors: André M
authors: AM
authors: Deelder
mesh_terms: D000595:Amino Acid Sequence; D053305:Apolipoprotein C-III / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification; D002236:Carbohydrate Conformation; D056148:Chromatography, Reverse-Phase; D005583:Fourier Analysis; D006020:Glycopeptides / Q000737:chemistry / Q000302:isolation & purification; D006031:Glycosylation; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D010449:Peptide Mapping / Q000379:methods*; D020033:Protein Isoforms / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification; D011499:Protein Processing, Post-Translational; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D053305:Apolipoprotein C-III; D006020:Glycopeptides; D010446:Peptide Fragments; D020033:Protein Isoforms