title: Free radical scavenging potential of Lagenaria siceraria (Molina) Standl fruits extract.
abstract: OBJECTIVE
  To elucidate free radical scavenging activity of ethanolic extract Lagenaria siceraria (L. siceraria) (Molina) fruit.
  
  
  METHODS
  The free radical scavenging activity of the L. siceraria (Molina) fruit extract was assayed by using α,α-diphenyl-β-picrylhydrazyl (DPPH), 2,20-azinobis 3-ethyl benzothiazoline-6-sulfonate (ABTS), FRAP, reducing power, chelating ability and β-carotene bleaching assay.
  
  
  RESULTS
  The IC(50) values of DPPH and ABTS radical-scavenging activity was found to be 1.95 mg/mL and 19 mg/mL, respectively. In ferrous chelation assay, the percentage of inhibition was found to be 89.21%. The reducing power of ethanolic extract of L. siceraria (Molina) fruit was 0.068 at 1 mg/mL and increased to 0.192 at 5 mg/mL. The β-carotene linoleate bleaching assay was 46.7% at 5 mg/mL and antioxidant activity using FRAP at 0.305 for 1 mg/mL to 0.969 for 5 mg/mL.
  
  
  CONCLUSIONS
  The results indicate that L. siceraria (Molina) fruit could be an important sources of natural radical scavengers.
authors: Institute of Biological Science, Faculty of Science, University of Malaya, Kuala Lumpur - 50603, Malaysia. drvijay@um.edu.my
authors: Vijayakumar
authors: V
authors: Mayakrishnan
authors: Selvi
authors: S
authors: Veluswamy
authors: Krishnakumari Shanmuga
authors: KS
authors: Sundaram
authors: Priya
authors: P
authors: Kannappan
authors: Noorlidah
authors: N
authors: Abdullah
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D019662:Cucurbitaceae / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D007502:Iron Chelating Agents / Q000737:chemistry / Q000494:pharmacology; D010084:Oxidation-Reduction / Q000187:drug effects; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D013451:Sulfonic Acids; D013844:Thiazoles; D019207:beta Carotene / Q000737:chemistry
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D007502:Iron Chelating Agents; D010851:Picrates; D010936:Plant Extracts; D013451:Sulfonic Acids; D013844:Thiazoles; D019207:beta Carotene; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl