title: Analysis of the effect of integrons on drug-resistant Staphylococcus             aureus by multiplex PCR detection.
abstract: The aim of this study was to detect class Ⅰ, II and III integrons using             multiplex PCR, and to analyze the role that these integrons play in mediating             multidrug-resistant Staphylococcus aureus (SA). The sensitivity of SA to 20 types             of antibiotics was examined using the K-B method. A genomic DNA extraction kit             was used for extracting genomic DNA and a high-purity 96 plasmid extraction kit             was used for extracting plasmid DNA. Class Ⅰ, II and III integrons were amplified             using multiplex PCR. Agarose gel electrophoresis was used for analysing amplification             products. The positive rate of class Ⅰ and II integrons in the plasmid DNA from             SA was higher compared to that of the genomic DNA. The positive rate of class Ⅰ             integrons was highest in the group with multidrug resistance to amoxicillin/clavulanic             acid, piperacillin/tazobactam, ciprofloxacin, tetracycline, rifampin, imipenem,             cefazolin, cefuroxime, levofloxacin and gentamicin. As regards integron detection             in the plasmids from drug-resistant SA strians obtained from sputum, blood, cerebrospinal             fluid, drainage fluid, excretion and urine specimens, the difference in the detection             rate of class Ⅰ integrons among the six types of specimens was significant. Multiplex             PCR is an effective method to detect class Ⅰ, II and III integrons. The SA plasmid             is the main carrier transferring integrons. Integrons mediate the formation of             SA multidrug resistance.
authors: Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, PR China.
authors: Chunfeng
authors: C
authors: Ren
authors: Yongjing
authors: Y
authors: Zhao
authors: Yan
authors: Y
authors: Shen
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000032:analysis*; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects / Q000235:genetics; D006801:Humans; D032023:Integrons / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D060885:Multiplex Polymerase Chain Reaction; D010957:Plasmids / Q000032:analysis / Q000235:genetics; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial