title: Subtyping Brazilian Yersinia pestis strains by pulsed-field gel electrophoresis.
abstract: We subtyped Brazilian Yersinia pestis strains by pulsed-field gel electrophoresis (PFGE). This was done with 22 Brazilian Y. pestis strains: 17 from an outbreak and 5 from endemic routine surveillance. The strains were divided into 2 groups (I and II), 8 subgroups (A-H) and 19 PFGE profiles or pulsotypes. PFGE did not separate outbreak from non-outbreak strains, as identical pulsotype patterns were found among outbreak strains and strains obtained from surveillance. However, it was able to detect intraspecific genetic diversity among Brazilian strains. This PFGE technique was able to differentiate a homogeneous group of Brazilian Y. pestis strains.
authors: Departamento de Microbiologia, Centro de Pesquisas Aggeu Magalhães, FIOCRUZ, Recife, PE, Brasil.
authors: M P S
authors: MP
authors: Barros
authors: V M
authors: VM
authors: Silveira-Filho
authors: R H F B
authors: RH
authors: Lins
authors: M B M
authors: MB
authors: Oliveira
authors: A M P
authors: AM
authors: Almeida
authors: T C
authors: TC
authors: Leal-Balbino
mesh_terms: D015373:Bacterial Typing Techniques; D001938:Brazil; D016000:Cluster Analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D005843:Geography; D015203:Reproducibility of Results; D015010:Yersinia pestis / Q000145:classification* / Q000235:genetics