title: rsEGFP2 enables fast RESOLFT nanoscopy of living cells.
abstract: The super-resolution microscopy called RESOLFT relying on fluorophore switching between longlived states, stands out by its coordinate-targeted sequential sample interrogation using low light levels. While RESOLFT has been shown to discern nanostructures in living cells, the reversibly photoswitchable green fluorescent protein (rsEGFP) employed in these experiments was switched rather slowly and recording lasted tens of minutes. We now report on the generation of rsEGFP2 providing faster switching and the use of this protein to demonstrate 25-250 times faster recordings.DOI:http://dx.doi.org/10.7554/eLife.00248.001.
authors: Department of NanoBiophotonics ,  Max Planck Institute for Biophysical Chemistry ,  Göttingen ,  Germany.
authors: Tim
authors: T
authors: Grotjohann
authors: Ilaria
authors: I
authors: Testa
authors: Matthias
authors: M
authors: Reuss
authors: Tanja
authors: T
authors: Brakemann
authors: Christian
authors: C
authors: Eggeling
authors: Stefan W
authors: SW
authors: Hell
authors: Stefan
authors: S
authors: Jakobs
mesh_terms: D000818:Animals; D002460:Cell Line; D004847:Epithelial Cells / Q000378:metabolism / Q000648:ultrastructure*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D007668:Kidney / Q000378:metabolism / Q000648:ultrastructure; D008027:Light; D007614:Macropodidae; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
chemical_list: D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins
keywords: GFP; None; confocal microscopy; fluorescent probes; live-cell imaging; nanoscopy; superresolution