title: Molecular characterization of high-level-cholera-toxin-producing El Tor variant Vibrio cholerae strains in the Zanzibar Archipelago of Tanzania.
abstract: Analysis of 1,180 diarrheal stool samples in Zanzibar detected 247 Vibrio cholerae O1, Ogawa strains in 2009. Phenotypic traits and PCR-based detection of rstR, rtxC, and tcpA alleles showed that they belonged to the El Tor biotype. Genetic analysis of ctxB of these strains revealed that they were classical type, and production of classical cholera toxin B (CTB) was confirmed by Western blotting. These strains produced more CT than the prototype El Tor and formed a separate cluster by pulsed-field gel electrophoresis (PFGE) analysis.
authors: National Institute of Cholera and Enteric Diseases, Kolkata, India.
authors: A
authors: A
authors: Naha
authors: G
authors: G
authors: Chowdhury
authors: J
authors: J
authors: Ghosh-Banerjee
authors: M
authors: M
authors: Senoh
authors: T
authors: T
authors: Takahashi
authors: B
authors: B
authors: Ley
authors: K
authors: K
authors: Thriemer
authors: J
authors: J
authors: Deen
authors: L V
authors: LV
authors: Seidlein
authors: S M
authors: SM
authors: Ali
authors: A
authors: A
authors: Khatib
authors: T
authors: T
authors: Ramamurthy
authors: R K
authors: RK
authors: Nandy
authors: G B
authors: GB
authors: Nair
authors: Y
authors: Y
authors: Takeda
authors: A K
authors: AK
authors: Mukhopadhyay
mesh_terms: D015153:Blotting, Western; D002771:Cholera / Q000453:epidemiology* / Q000382:microbiology*; D002772:Cholera Toxin / Q000378:metabolism*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005243:Feces / Q000382:microbiology; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D058889:Molecular Typing; D017422:Sequence Analysis, DNA; D013636:Tanzania / Q000453:epidemiology; D041241:Vibrio cholerae O1 / Q000302:isolation & purification* / Q000472:pathogenicity
chemical_list: D004269:DNA, Bacterial; D002772:Cholera Toxin