title: Knockout of pgdS and ggt genes improves γ-PGA yield in B. subtilis.
abstract: One of the emerging biopolymers that are currently under active investigation is bacterial poly(γ-glutamic acid) (γ-PGA). However, before its full industrial exploitation, a substantial increase in microbial productivity is required. γ-PGA obtained from the Bacillus subtilis laboratory strain 168 offers the advantage of a producer characterized by a well defined genetic framework and simple manipulation techniques. In this strain, the knockout of genes for the major γ-PGA degrading enzymes, pgdS and ggt, leads to a considerable improvement in polymer yield, which attains levels analogous to the top wild γ-PGA producer strains. This study highlights the convenience of using the laboratory strain of B. subtilis over wild isolates in designing strain improvement strategies aimed at increasing γ-PGA productivity.
authors: Department of Biology and Biotechnology, Università degli Studi di Pavia, Via Ferrata 1, Pavia (I) 27100, Italy.
authors: Viola
authors: V
authors: Scoffone
authors: Daniele
authors: D
authors: Dondi
authors: Ginevra
authors: G
authors: Biino
authors: Giovanni
authors: G
authors: Borghese
authors: Dario
authors: D
authors: Pasini
authors: Alessandro
authors: A
authors: Galizzi
authors: Cinzia
authors: C
authors: Calvio
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics*; D001709:Biotechnology / Q000379:methods; D055786:Gene Knockout Techniques; D060847:Metabolic Engineering / Q000379:methods*; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis; D015854:Up-Regulation
chemical_list: D001426:Bacterial Proteins; C511775:poly(gamma-glutamic acid); D011099:Polyglutamic Acid