title: Iron overload inhibits osteoblast biological activity through oxidative stress.
abstract: Iron overload has recently been connected with bone mineral density in osteoporosis. However, to date, the effect of iron overload on osteoblasts remains poorly understood. The purpose of this study is to examine osteoblast biological activity under iron overload. The osteoblast cells (hFOB1.19) were cultured in a medium supplemented with different concentrations (50, 100, and 200 μM) of ferric ammonium citrate as a donor of ferric ion. Intracellular iron was measured with a confocal laser scanning microscope. Reactive oxygen species (ROS) were detected by 2,7-dichlorofluorescin diacetate fluorophotometry. Osteoblast biological activities were evaluated by measuring the activity of alkaline phosphatase (ALP) and mineralization function. Results indicated that iron overload could consequently increase intracellular iron concentration and intracellular ROS levels in a concentration-dependent manner. Additionally, ALP activity was suppressed, and a decline in the number of mineralized nodules was observed in in vitro cultured osteoblast cells. According to these results, it seems that iron overload probably inhibits osteoblast function through higher oxidative stress following increased intracellular iron concentrations.
authors: Department of Orthopaedics, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors: Yin-Feng
authors: YF
authors: He
authors: Yong
authors: Y
authors: Ma
authors: Chao
authors: C
authors: Gao
authors: Guo-Yang
authors: GY
authors: Zhao
authors: Lin-Lin
authors: LL
authors: Zhang
authors: Guang-Fei
authors: GF
authors: Li
authors: Yun-Zhi
authors: YZ
authors: Pan
authors: Kai
authors: K
authors: Li
authors: You-Jia
authors: YJ
authors: Xu
mesh_terms: D002460:Cell Line; D006801:Humans; D007501:Iron / Q000633:toxicity*; D019190:Iron Overload / Q000378:metabolism* / Q000503:physiopathology; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D007501:Iron