title: New platform technology for comprehensive serological diagnostics of autoimmune diseases.
abstract: Antibody assessment is an essential part in the serological diagnosis of autoimmune diseases. However, different diagnostic strategies have been proposed for the work up of sera in particular from patients with systemic autoimmune rheumatic disease (SARD). In general, screening for SARD-associated antibodies by indirect immunofluorescence (IIF) is followed by confirmatory testing covering different assay techniques. Due to lacking automation, standardization, modern data management, and human bias in IIF screening, this two-stage approach has recently been challenged by multiplex techniques particularly in laboratories with high workload. However, detection of antinuclear antibodies by IIF is still recommended to be the gold standard method for antibody screening in sera from patients with suspected SARD. To address the limitations of IIF and to meet the demand for cost-efficient autoantibody screening, automated IIF methods employing novel pattern recognition algorithms for image analysis have been introduced recently. In this respect, the AKLIDES technology has been the first commercially available platform for automated interpretation of cell-based IIF testing and provides multiplexing by addressable microbead immunoassays for confirmatory testing. This paper gives an overview of recently published studies demonstrating the advantages of this new technology for SARD serology.
authors: Institute of Molecular and Clinical Immunology, Otto-von-Guericke University, 39120 Magdeburg, Germany.
authors: Annika
authors: A
authors: Willitzki
authors: Rico
authors: R
authors: Hiemann
authors: Vanessa
authors: V
authors: Peters
authors: Ulrich
authors: U
authors: Sack
authors: Peter
authors: P
authors: Schierack
authors: Stefan
authors: S
authors: Rödiger
authors: Ursula
authors: U
authors: Anderer
authors: Karsten
authors: K
authors: Conrad
authors: Dimitrios P
authors: DP
authors: Bogdanos
authors: Dirk
authors: D
authors: Reinhold
authors: Dirk
authors: D
authors: Roggenbuck
mesh_terms: D001323:Autoantibodies / Q000737:chemistry* / Q000276:immunology*; D001327:Autoimmune Diseases / Q000175:diagnosis* / Q000276:immunology*; D019084:Fluorescent Antibody Technique, Indirect / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D012698:Serologic Tests / Q000295:instrumentation / Q000379:methods*
chemical_list: D001323:Autoantibodies