title: Protective effect of oestradiol in the coeliac ganglion against ovarian apoptotic mechanism on dioestrus.
abstract: The aims of this work were to investigate if oestradiol 10(-8)M in the incubation media of either the ovary alone (OV) or the ganglion compartment of an ex vivo coeliac ganglion-superior ovarian nerve-ovary system (a) modifies the release of ovarian progesterone (P4) and oestradiol (E2) on dioestrus II, and (b) modifies the ovarian gene expression of 3β-HSD and 20α-HSD enzymes and markers of apoptosis. The concentration of ovarian P4 release was measured in both experimental schemes, and ovarian P4 and E2 in the ex vivo system by RIA at different times. The expression of 3β-hydroxysteroid dehydrogenase, 20α-hydroxysteroid dehydrogenase and antiapoptotic bcl-2 and proapoptotic bax by RT-PCR were determined. E2 added in the coeliac ganglion caused an increase in the ovarian release of the P4, E2 and 3β-HSD, while in the ovary incubation alone it decreased P4 and 3β-HSD but increased and 20α-HSD and bax/bcl-2 ratio. It is concluded that through a direct effect on the ovary, E2 promotes luteal regression in DII rats, but the addition of E2 in the coeliac ganglion does not have the same effect. The peripheral nervous system, through the superior ovarian nerve, has a protective effect against the apoptotic mechanism on DII.
authors: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Química, Bioquímica y Farmacia, UNSL, San Luis, Argentina. zsosa@unsl.edu.ar
authors: Bronzi
authors: B
authors: Cynthia
authors: Daneri Becerra
authors: DB
authors: Cristina
authors: Vega Orozco
authors: VO
authors: Adriana
authors: Delsouc María
authors: DM
authors: Belén
authors: Rastrilla Ana
authors: RA
authors: María
authors: Casais
authors: C
authors: Marilina
authors: Sosa
authors: S
authors: Zulema
mesh_terms: D043607:20-alpha-Hydroxysteroid Dehydrogenase / Q000235:genetics / Q000378:metabolism; D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004031:Diestrus; D004958:Estradiol / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D005728:Ganglia, Sympathetic / Q000187:drug effects* / Q000378:metabolism; D010053:Ovary / Q000187:drug effects / Q000294:innervation / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism; D051381:Rats; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D051025:bcl-Associated Death Protein / Q000096:biosynthesis / Q000378:metabolism
chemical_list: C494828:Bax protein, rat; D051028:bcl-2-Associated X Protein; D051025:bcl-Associated Death Protein; D011374:Progesterone; D004958:Estradiol; D015096:3-Hydroxysteroid Dehydrogenases; D043607:20-alpha-Hydroxysteroid Dehydrogenase