title: Development and optimization of an integrated PDMS based-microdialysis microchip electrophoresis device with on-chip derivatization for continuous monitoring of primary amines.
abstract: An all-PDMS on-line microdialysis-microchip electrophoresis with on-chip derivatization and electrophoretic separation for near real-time monitoring of primary amine-containing analytes is described. Each part of the chip was optimized separately, and the effect of each of the components on temporal resolution, lag time, and separation efficiency of the device was determined. Aspartate and glutamate were employed as test analytes. Derivatization was accomplished with naphthalene-2,3,-dicarboxyaldehyde/cyanide (NDA/CN(-)), and the separation was performed using a 15-cm serpentine channel. The analytes were detected using LIF detection.
authors: Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS, USA.
authors: Pradyot
authors: P
authors: Nandi
authors: David E
authors: DE
authors: Scott
authors: Dhara
authors: D
authors: Desai
authors: Susan M
authors: SM
authors: Lunte
mesh_terms: D000588:Amines / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis*; D001224:Aspartic Acid / Q000032:analysis; D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods*; D018698:Glutamic Acid / Q000032:analysis; D017551:Microdialysis / Q000295:instrumentation / Q000379:methods; D009281:Naphthalenes / Q000032:analysis / Q000737:chemistry
chemical_list: D000588:Amines; D000596:Amino Acids; D009281:Naphthalenes; D001224:Aspartic Acid; D018698:Glutamic Acid; C027717:2,3-naphthalenedicarboxaldehyde