title: The FPase properties and morphology changes of a cellulolytic bacterium, Sporocytophaga sp. JL-01, on decomposing filter paper cellulose.
abstract: Sporocytophaga sp. JL-01 is a sliding cellulose degrading bacterium that can decompose filter paper (FP), carboxymethyl cellulose (CMC) and cellulose CF11. In this paper, the morphological characteristics of S. sp. JL-01 growing in FP liquid medium was studied by Scanning Electron Microscope (SEM), and one of the FPase components of this bacterium was analyzed. The results showed that the cell shapes were variable during the process of filter paper cellulose decomposition and the rod shape might be connected with filter paper decomposing. After incubating for 120 h, the filter paper was decomposed significantly, and it was degraded absolutely within 144 h. An FPase1 was purified from the supernatant and its characteristics were analyzed. The molecular weight of the FPase1 was 55 kDa. The optimum pH was pH 7.2 and optimum temperature was 50°C under experiment conditions. Zn(2+) and Co(2+) enhanced the enzyme activity, but Fe(3+) inhibited it.
authors: School of Life Science, Northeast Norma University, 130024, Changchun, China.
authors: Xiuran
authors: X
authors: Wang
authors: Zhongqi
authors: Z
authors: Peng
authors: Xiaoling
authors: X
authors: Sun
authors: Dongbo
authors: D
authors: Liu
authors: Shan
authors: S
authors: Chen
authors: Fan
authors: F
authors: Li
authors: Hongmei
authors: H
authors: Xia
authors: Tiancheng
authors: T
authors: Lu
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D002266:Carboxymethylcellulose Sodium / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D041964:Cytophagaceae / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification / Q000648:ultrastructure*; D005374:Filtration; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D010209:Paper; D012988:Soil Microbiology; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D002482:Cellulose; D002480:Cellulase; D002266:Carboxymethylcellulose Sodium