title: Simultaneous detection and identification of four viruses infecting pepino by multiplex RT-PCR.
abstract: Potato virus M (PVM), pepino mosaic virus (PepMV), tomato mosaic virus (ToMV), and potato virus S (PVS) infect pepino and cause serious crop losses. In this study, a multiplex RT-PCR method was developed for simultaneous detection and differentiation of PVS, ToMV, PepMV and PVM. The method was highly reliable and sensitive; validation was accomplished by testing pepino samples collected from different regions of China. In this survey, PVM, ToMV and PVS were detected in 37.0 %, 31.0 % and 5.5 % of samples tested, respectively, confirming the widespread occurrence of these three viruses in China. PepMV was not detected in any of the samples, which indicated that this virus may not be prevalent in China. The results suggest that the new multiplex RT-PCR method has potential to be used routinely for surveys of pepino for virus infection.
authors: College of Agronomy and Biotechnology, China Agricultural University, Yuanmingyuan West Road No. 2, Haidian District, Beijing 100193, People's Republic of China.
authors: Beibei
authors: B
authors: Ge
authors: Qian
authors: Q
authors: Li
authors: Guojie
authors: G
authors: Liu
authors: Meiguang
authors: M
authors: Lu
authors: Shifang
authors: S
authors: Li
authors: Hongqing
authors: H
authors: Wang
mesh_terms: D017788:Carlavirus / Q000235:genetics*; D002681:China; D004797:Enzyme-Linked Immunosorbent Assay; D060885:Multiplex Polymerase Chain Reaction; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D017863:Potexvirus / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D032322:Solanum / Q000821:virology*; D017898:Tobamovirus / Q000235:genetics*