title: Alterations in the MA and NC domains modulate phosphoinositide-dependent plasma membrane localization of the Rous sarcoma virus Gag protein.
abstract: Retroviral Gag proteins direct virus particle assembly from the plasma membrane (PM). Phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P(2)] plays a role in PM targeting of several retroviral Gag proteins. Here we report that depletion of intracellular PI(4,5)P(2) and phosphatidylinositol-(3,4,5)-triphosphate [PI(3,4,5)P(3)] levels impaired Rous sarcoma virus (RSV) Gag PM localization. Gag mutants deficient in nuclear trafficking were less sensitive to reduction of intracellular PI(4,5)P(2) and PI(3,4,5)P(3), suggesting a possible connection between Gag nuclear trafficking and phosphoinositide-dependent PM targeting.
authors: Department of Medicine, Penn State College of Medicine, Hershey, PA, USA.
authors: Shorena
authors: S
authors: Nadaraia-Hoke
authors: Darrin V
authors: DV
authors: Bann
authors: Timothy L
authors: TL
authors: Lochmann
authors: Nicole
authors: N
authors: Gudleski-O'Regan
authors: Leslie J
authors: LJ
authors: Parent
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D015683:Gene Products, gag / Q000235:genetics / Q000378:metabolism*; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D052736:Rous sarcoma virus / Q000502:physiology*; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D019065:Virus Assembly
chemical_list: D015683:Gene Products, gag; C110263:MA protein, Rous sarcoma virus; D050505:Mutant Proteins; D010716:Phosphatidylinositols; D010750:Phosphoproteins; D014763:Viral Matrix Proteins