title: Using protease inhibitors in antigen presentation assays.
abstract: The major histocompatibility complex (MHC) class I restricted pathway of antigen processing allows the presentation of intracellular antigens to cytotoxic T lymphocytes. The proteasome is the main protease in the cytoplasm and the nucleus, which is responsible for the generation of most peptide ligands of MHC-I molecules. Peptides produced by the proteasome can be further trimmed or destroyed by numerous cytosolic or endoplasmic reticulum (ER) lumenal proteases. Small molecule inhibitors are useful tools for probing the role of proteases in MHC class I antigen processing. Here, we describe different methods to test the impact of protease inhibitors in antigen presentation assays.
authors: Biotechnology Institute Thurgau at the University of Constance, Kreuzlingen, Switzerland. michael.basler@uni-konstanz.de.|Department of Biology, Division of Immunology, University of Konstanz, Konstanz, Germany. michael.basler@uni-konstanz.de.
authors: Michael
authors: M
authors: Basler
authors: Biotechnology Institute Thurgau at the University of Konstanz, Kreuzlingen, Switzerland.|Division of Immunology, Department of Biology, University of Konstanz, Konstanz, Germany.
authors: Marcus
authors: M
authors: Groettrup
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000187:drug effects*; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D003584:Cytological Techniques / Q000379:methods*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D015234:HLA-A Antigens / Q000276:immunology; D006825:Hybridomas / Q000166:cytology; D042541:Intracellular Space / Q000187:drug effects; D007763:Lac Operon / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011480:Protease Inhibitors / Q000494:pharmacology*; D013194:Staining and Labeling; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000378:metabolism
chemical_list: D016207:Cytokines; D018984:Epitopes, T-Lymphocyte; D015234:HLA-A Antigens; D011480:Protease Inhibitors