title: Optimization of ultrasonic assisted extraction of antioxidants from black soybean (Glycine max var) sprouts using response surface methodology.
abstract: Response surface methodology (RSM) using a central composite design (CCD) was employed to optimize the conditions for extraction of antioxidants from black soybean (Glycine max var) sprouts. Three influencing factors: liquid-solid ratio, period of ultrasonic assisted extraction and extraction temperature were investigated in the ultrasonic aqueous extraction. Then Response Surface Methodology (RSM) was applied to optimize the extraction process focused on DPPH radical-scavenging capacity of the antioxidants with respect to the above influencing factors. The best combination of each significant factor  was determined by RSM design and optimum pretreatment conditions for maximum radical-scavenging capacity were established to be liquid-solid ratio of 29.19:1, extraction time of 32.13 min, and extraction temperature of 30 °C. Under these conditions, 67.60% of DPPH radical-scavenging capacity was observed experimentally, similar to the theoretical prediction of 66.36%.
authors: School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, Jiangsu, China.
authors: Jixiang
authors: J
authors: Lai
authors: Can
authors: C
authors: Xin
authors: Ya
authors: Y
authors: Zhao
authors: Bing
authors: B
authors: Feng
authors: Congfen
authors: C
authors: He
authors: Yinmao
authors: Y
authors: Dong
authors: Yun
authors: Y
authors: Fang
authors: Shaomin
authors: S
authors: Wei
mesh_terms: D001713:Biphenyl Compounds / Q000737:chemistry; D003198:Computer Simulation; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification*; D005609:Free Radicals / Q000737:chemistry; D008956:Models, Chemical; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods; D013016:Sound; D013025:Glycine max / Q000737:chemistry*
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl