title: [Isolation of aerobic degrading strains for TBBPA and the properties of biodegradation].
abstract: In order to investigate the characteristics of the microbial degradation of tetrabromobisphenol A (TBBPA), four aerobic strains were isolated from the phenol granular sludge using selective medium with TBBPA as the sole carbon source. Among them, strain H could accommodate the TBBPA culture medium and degrade TBBPA with high efficiency, which was identified as Rhodococcus sp. via DNA sequencing test. The optimal conditions for TBBPA degradation by strain H was pH 6.5, 30 degrees C, 150 r x min(-1), the concentration of humic acid being 200 mg x L(-1) and the concentration of K+ being 1 000 mg x L(-1). Under the optimal conditions, the debromination rate of TBBPA reached 20.75% after 21 d. The results of LC-MS showed that the major degradation product was monobromophenol, which was formed by the removal of isopropyl from the benzene ring in TBBPA molecule. The results of protein gel electrophoresis (SDS-PAGE) showed that strain H had a protein band between (90-117) x 10(3) kDa, which might be the enzyme responsible for TBBPA degradation.
authors: School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. qianyanyuan@sjtu.edu.cn
authors: Yan-Yuan
authors: YY
authors: Qian
authors: Li-Li
authors: LL
authors: Liu
authors: Xiao-Juan
authors: XJ
authors: Yu
authors: Cong
authors: C
authors: Ding
authors: Zhi-Ping
authors: ZP
authors: Wang
authors: Yu-Heng
authors: YH
authors: Shi
authors: Chen-Jun
authors: CJ
authors: Li
mesh_terms: D001420:Bacteria, Aerobic / Q000378:metabolism*; D001673:Biodegradation, Environmental; D011075:Polybrominated Biphenyls / Q000302:isolation & purification* / Q000378:metabolism; D012240:Rhodococcus / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology
chemical_list: D011075:Polybrominated Biphenyls; C020806:tetrabromobisphenol A