title: Fatigue-related gene networks identified in CD(14)+ cells isolated from HIV-infected patients: part I: research findings.
abstract: OBJECTIVE
  Human immunodeficiency virus (HIV)-related fatigue (HRF) is multicausal and potentially related to mitochondrial dysfunction caused by antiretroviral therapy with nucleoside reverse transcriptase inhibitors (NRTIs).
  
  
  METHODS
  The authors compared gene expression profiles of CD14(+) cells of low versus high fatigued, NRTI-treated HIV patients to healthy controls (n = 5/group). The authors identified 32 genes predictive of low versus high fatigue and 33 genes predictive of healthy versus HIV infection. The authors constructed genetic networks to further elucidate the possible biological pathways in which these genes are involved. RELEVANCE FOR NURSING PRACTICE: Genes including the actin cytoskeletal regulatory proteins Prokineticin 2 and Cofilin 2 along with mitochondrial inner membrane proteins are involved in multiple pathways and were predictors of fatigue status. Previously identified inflammatory and signaling genes were predictive of HIV status, clearly confirming our results and suggesting a possible further connection between mitochondrial function and HIV. Isolated CD14(+) cells are easily accessible cells that could be used for further study of the connection between fatigue and mitochondrial function of HIV patients.
  
  
  CONCLUSIONS
  The findings from this pilot study take us one step closer to identifying biomarker targets for fatigue status and mitochondrial dysfunction. Specific biomarkers will be pertinent to the development of methodologies to diagnosis, monitor, and treat fatigue and mitochondrial dysfunction.
authors: Biobehavioral Nursing & Health Systems Department, University of Washington, School of Nursing, Seattle, WA 98195, USA. vossj@uw.edu
authors: Joachim G
authors: JG
authors: Voss
authors: Adrian
authors: A
authors: Dobra
authors: Caryn
authors: C
authors: Morse
authors: Joseph A
authors: JA
authors: Kovacs
authors: Robert L
authors: RL
authors: Danner
authors: Peter J
authors: PJ
authors: Munson
authors: Carolea
authors: C
authors: Logan
authors: Zoila
authors: Z
authors: Rangel
authors: Joseph W
authors: JW
authors: Adelsberger
authors: Mary
authors: M
authors: McLaughlin
authors: Larry D
authors: LD
authors: Adams
authors: Raghavan
authors: R
authors: Raju
authors: Marinos C
authors: MC
authors: Dalakas
mesh_terms: D015153:Blotting, Western; D005221:Fatigue / Q000276:immunology*; D015658:HIV Infections / Q000188:drug therapy / Q000276:immunology / Q000503:physiopathology*; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000276:immunology*; D018894:Reverse Transcriptase Inhibitors / Q000627:therapeutic use; D015398:Signal Transduction
chemical_list: D018950:Lipopolysaccharide Receptors; D018894:Reverse Transcriptase Inhibitors