title: Development of a new multi-walled carbon nanotube (MWCNT) aerosol generation and exposure system and confirmation of suitability for conducting a single-exposure inhalation study of MWCNT in rats.
abstract: Because the primary route of human exposure to multi-walled carbon nanotube (MWCNT) is via inhalation, a new dry MWCNT aerosol generation and exposure system for whole-body inhalation exposure using a cyclone and sieve has been developed. The system was tested for operational performance at 0.2, 1 and 5 mg/m(3). Additionally, it was examined whether this system can be employed in animal whole-body inhalation studies by exposing rats to MWCNT aerosol for 6 h at 5 mg/m(3). The system could consistently provide aerosols with a similar particle size distribution and configuration at all the target exposure concentrations. Almost all MWCNTs were fibrous, and the presence of many well-dispersed, nano-sized particles was confirmed. Additionally, the animal study revealed that large amounts of MWCNTs were inhaled into the lung, resulting in an inflammatory response, with increased LDH and albumin levels, and granulomatous change. Therefore, the aerosol generation and exposure system appears useful for MWCNT inhalation studies using rats.
authors: Japan Bioassay Research Center, Japan Industrial Safety and Health Association , 2445 Hirasawa, Hadano, Kanagawa 257-0015 , Japan.
authors: Tatsuya
authors: T
authors: Kasai
authors: Kaoru
authors: K
authors: Gotoh
authors: Tomoshi
authors: T
authors: Nishizawa
authors: Toshiaki
authors: T
authors: Sasaki
authors: Taku
authors: T
authors: Katagiri
authors: Yumi
authors: Y
authors: Umeda
authors: Tadao
authors: T
authors: Toya
authors: Shoji
authors: S
authors: Fukushima
mesh_terms: D000336:Aerosols / Q000633:toxicity*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D004867:Equipment Design; D019570:Inhalation Exposure / Q000032:analysis*; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008297:Male; D037742:Nanotubes, Carbon / Q000633:toxicity*; D010316:Particle Size; D051381:Rats; D011916:Rats, Inbred F344; D018675:Toxicity Tests / Q000295:instrumentation* / Q000379:methods*
chemical_list: D000336:Aerosols; D037742:Nanotubes, Carbon