title: Studying MHC class II peptide loading and editing in vitro.
abstract: HLA-DM is now known to have a major contribution to the selection of immunodominant epitopes. A better understanding of the mechanisms controlling epitope selection can be achieved by examination of the biophysical behavior of major histocompatibility complex (MHC) class II molecules upon binding of antigenic peptides and the effect of DM on the interactions. Using purified soluble molecules, in this chapter, we describe several in vitro methods for measuring peptide binding to HLA-DR molecules and the effects of HLA-DM on the interactions. A simple qualitative method, Gentle SDS-PAGE               Assay, would assess the ability of peptides to form tight complexes with MHC class II molecules. Measuring binding kinetics is among the most informative approaches to understanding molecular mechanisms, and here we describe two different methods for measuring binding kinetics of peptide-MHC complexes. In one method, rates of association and dissociation of fluorescently labeled peptides to soluble MHC class II molecules can be determined using G50 spin columns to separate unbound peptides from those in complex with MHC molecules. In another method, association and dissociation of unlabeled peptides and MHC class II molecules can be determined in real time using BIAcore surface plasmon               resonance (SPR). We also have described an Intrinsic Tryptophan Fluorescence Assay for studying transient interactions of DM and MHC class II molecules.
authors: Department of Pathology, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.
authors: AeRyon
authors: A
authors: Kim
authors: Department of Pathology, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.
authors: Isabel
authors: I
authors: Ishizuka
authors: Department of Pathology, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.
authors: Isamu
authors: I
authors: Hartman
authors: Department of Pathology, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.
authors: Yuri
authors: Y
authors: Poluektov
authors: Department of Pathology, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.
authors: Kedar
authors: K
authors: Narayan
authors: Department of Pathology, School of Medicine, Johns Hopkins University, Baltimore, MD, USA. ssadegh@jhmi.edu.
authors: Scheherazade
authors: S
authors: Sadegh-Nasseri
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D006681:HLA-D Antigens / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D059811:HLA-DRB1 Chains / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012995:Solubility; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D006681:HLA-D Antigens; C084245:HLA-DM antigens; D059811:HLA-DRB1 Chains; D055767:Immobilized Proteins; D010446:Peptide Fragments