title: Evaluation of antibacterial efficacy of phyto fabricated silver nanoparticles using Mukia scabrella (Musumusukkai) against drug resistance nosocomial gram negative bacterial pathogens.
abstract: Given the fact in the limitation of the therapeutic options for emerging multidrug resistance gram-negative bacteria (MDR-GNB) of respiratory tract infections, the present study was focused on green synthesis of antimicrobial silver nanoparticles (AgNPs) using leaf extract of Mukia scabrella. An obvious color change to brown color and surface plasmon resonance by UV-visible spectroscopy (UV-vis) indicated a well observable peak at 440 nm confirming the synthesis of AgNPs. Fourier transform infra-red spectroscopy (FTIR) analysis indicates protein as possible capping agents. Energy dispersive X-ray (EDAX) spectroscopy results showed major signal for elemental silver. X-ray diffraction (XRD) analysis indicates the formation of metallic silver nanomaterials. Transmission electron microscopic (TEM) study showed the nanoparticles in the size range of 18-21 nm with spherical shape. Zeta potential analysis showed -21.7 mV characteristic for stable AgNPs. The biosynthesized AgNPs exhibited significant antimicrobial activity against MDR-GNB nosocomial pathogens of Acinetobacter sp., Klebsiella pneumoniae and Pseudomonas aeruginosa. Results from the current study suggested that M. scabrella material could be exploited for the fabrication of AgNPs with potential therapeutic applications in nanomedicine especially for nosocomial bacterial infections.
authors: Postgraduate and Research Department of Zoology, Jamal Mohamed College, Tiruchirappalli 620020, Tamil Nadu, India. drpklab@gmail.com
authors: Kandasamy
authors: K
authors: Prabakar
authors: Periyasamy
authors: P
authors: Sivalingam
authors: Siyed Ibrahim
authors: SI
authors: Mohamed Rabeek
authors: Manickam
authors: M
authors: Muthuselvam
authors: Naresh
authors: N
authors: Devarajan
authors: Annavi
authors: A
authors: Arjunan
authors: Rajamanickam
authors: R
authors: Karthick
authors: Micky Maray
authors: MM
authors: Suresh
authors: John Pote
authors: JP
authors: Wembonyama
mesh_terms: D000150:Acinetobacter / Q000187:drug effects*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D024881:Drug Resistance, Bacterial / Q000187:drug effects*; D007711:Klebsiella pneumoniae / Q000187:drug effects*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D012834:Silver / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D012834:Silver