title: Sensitive chemiluminescence aptasensor based on exonuclease-assisted recycling amplification.
abstract: We report herein an exonuclease-assisted aptamer-based target recycling amplification strategy for sensitive and selective chemiluminescence (CL) determination of adenosine. This aptasensor is based on target-induced release of aptamers from capture probes immobilized on the 96-well plate surface, and thus leading to a decreased hybridization with gold nanoparticle-functionalized reporter sequences followed by a CL signal. The introduction of exonuclease III catalyzes the stepwise removal of mononucleotides from 3'-hydroxyl termini of duplex DNAs of aptamers, liberating the adenosine. Therefore, a single copy of target adenosine can lead to the release and digestion of numerous aptamer strands from the 96-well plates and ultimately an enhanced sensitivity is achieved. Experimental results revealed that the exonuclease-assisted recycling strategy enabled the monitoring of adenosine with wide working ranges and low detection limits (LOD: 0.5 nM). This new CL strategy might create a novel technology for the detection of various targets and could find wide applications in the environmental and biomedical fields.
authors: School of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education & PLA, Fudan University, 826 Zhangheng Road, Shanghai 201203, China.
authors: Sheng
authors: S
authors: Cai
authors: Yanhua
authors: Y
authors: Sun
authors: Choiwan
authors: C
authors: Lau
authors: Jianzhong
authors: J
authors: Lu
mesh_terms: D000241:Adenosine / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D052157:Aptamers, Nucleotide / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D057230:Limit of Detection; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods
chemical_list: D052157:Aptamers, Nucleotide; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III; D000241:Adenosine