title: O-GlcNAcylation of BMAL1 regulates circadian rhythms in NIH3T3 fibroblasts.
abstract: Various physiological processes and behaviors show a circadian rhythm of approximately 24 h, which is crucial in coordinating internal metabolic processes and environmental signals. Post-translational modifications play an important role in regulating circadian core proteins. In this study, we demonstrated that BMAL1 was modified with an O-linked β-N-acetylglucosamine (O-GlcNAc), which stabilized BMAL1 and enhanced its transcriptional activity. Conversely, inhibition of O-GlcNAcylation resulted in inhibition of circadian rhythms of clock gene expression. Because O-GlcNAcylation is sensitive to the glucose level, such a modification may provide a new mechanism connecting metabolism with circadian rhythms.
authors: State Key Laboratory of Medical Genetics, Central South University, Changsha, Hunan 410078, China.
authors: Yan-Tao
authors: YT
authors: Ma
authors: Hunjin
authors: H
authors: Luo
authors: Wen-Juan
authors: WJ
authors: Guan
authors: Hua
authors: H
authors: Zhang
authors: Chongfen
authors: C
authors: Chen
authors: Ziyao
authors: Z
authors: Wang
authors: Jia-Da
authors: JD
authors: Li
mesh_terms: D056930:ARNTL Transcription Factors / Q000235:genetics / Q000378:metabolism*; D000117:Acetylglucosamine / Q000378:metabolism*; D000215:Acylation / Q000187:drug effects; D000818:Animals; D002940:Circadian Rhythm / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D005347:Fibroblasts / Q000502:physiology; D017930:Genes, Reporter; D057809:HEK293 Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics; D051379:Mice; D041681:NIH 3T3 Cells; D055550:Protein Stability; D014158:Transcription, Genetic
chemical_list: D056930:ARNTL Transcription Factors; C538960:BMAL1 protein, human; D008156:Luciferases; D000117:Acetylglucosamine