title: Quercetin inhibits transcriptional up-regulation of histamine H1 receptor via suppressing protein kinase C-δ/extracellular signal-regulated kinase/poly(ADP-ribose) polymerase-1 signaling pathway in HeLa cells.
abstract: It has been reported that the histamine H1 receptor (H1R) gene is up-regulated in patients with allergic rhinitis and H1R expression level strongly correlates with the severity of allergy symptoms. Accordingly compounds that suppress the H1R gene expression are promising as useful anti-allergic medications. Recently, we demonstrated that histamine or phorbol-12-myristate-13-acetate (PMA) stimulation induced the up-regulation of H1R gene expression through the protein kinase Cδ (PKCδ)/extracellular signal-regulated kinase/poly(ADP-ribose) polymerase-1 signaling pathway in HeLa cells expressing H1R endogenously. Quercetin is one of the well-characterized flavonoids and it possesses many biological activities including anti-allergic activity. However, effect of quercetin on H1R signaling is remained unknown. In the present study, we examined the effect of quercetin on histamine- and PMA-induced up-regulation of H1R gene expression in HeLa cells. We also investigated its in vivo effects on the toluene-2,4-diisocyanate (TDI)-sensitized allergy model rats. Quercetin suppressed histamine- and PMA-induced up-regulation of H1R gene expression. Quercetin also inhibited histamine- or PMA-induced phosphorylation of Tyr(311) of PKCδ and translocation of PKCδ to the Golgi. Pre-treatment with quercetin for 3weeks suppressed TDI-induced nasal allergy-like symptoms and elevation of H1R mRNA in the nasal mucosa of TDI-sensitized rats. These data suggest that quercetin suppresses H1R gene expression by the suppression of PKCδ activation through the inhibition of its translocation to the Golgi.
authors: Department of Molecular Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8505, Japan.
authors: Masashi
authors: M
authors: Hattori
authors: Hiroyuki
authors: H
authors: Mizuguchi
authors: Yuko
authors: Y
authors: Baba
authors: Shohei
authors: S
authors: Ono
authors: Tomohiro
authors: T
authors: Nakano
authors: Qian
authors: Q
authors: Zhang
authors: Yohei
authors: Y
authors: Sasaki
authors: Makoto
authors: M
authors: Kobayashi
authors: Yoshiaki
authors: Y
authors: Kitamura
authors: Noriaki
authors: N
authors: Takeda
authors: Hiroyuki
authors: H
authors: Fukui
mesh_terms: D000818:Animals; D003600:Cytosol / Q000378:metabolism*; D003877:Dermatitis, Contact / Q000188:drug therapy*; D004195:Disease Models, Animal; D006056:Golgi Apparatus / Q000378:metabolism*; D006367:HeLa Cells; D006632:Histamine / Q000494:pharmacology; D006634:Histamine H1 Antagonists / Q000008:administration & dosage*; D006801:Humans; D008297:Male; D010703:Phorbol Esters / Q000494:pharmacology; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D051745:Protein Kinase C-delta / Q000378:metabolism*; D021381:Protein Transport; D011794:Quercetin / Q000008:administration & dosage*; D051381:Rats; D011919:Rats, Inbred Strains; D011969:Receptors, Histamine H1 / Q000235:genetics*; D015398:Signal Transduction / Q000187:drug effects; D014051:Toluene 2,4-Diisocyanate / Q000276:immunology; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D006634:Histamine H1 Antagonists; D010703:Phorbol Esters; D011969:Receptors, Histamine H1; D014051:Toluene 2,4-Diisocyanate; C032974:12-O-retinoylphorbol-13-acetate; D006632:Histamine; D011794:Quercetin; D011065:Poly(ADP-ribose) Polymerases; D051745:Protein Kinase C-delta