title: [Molecular analysis on non-O1 and non-O139 Vibrio cholerae isolates].
abstract: OBJECTIVE
  According to results from the two-month consecutive surveillance program in Maanshan, six suspected cases of non-O1 non-O139 Vibrio (V.) cholerae infection, were found that called for identification of pathogens as well as molecular-epidemiological analysis to determine the aggregation of the epidemic situation.
  
  
  METHODS
  Biochemical and serotype identification, hemolysis test, and drug sensitive test were used to detect the drug resistance spectrum. Real-time PCR and conventional PCR were used to detect the presence of V. cholerae specific genes, virulent genes and its related genes, including ompW, ctx, tcpA, toxR, hlyA, zot, ace, rstR and gIII(CTX). Pulsed-field gel electrophoresis (PFGE) was used to analyze the molecular type of strains.
  
  
  RESULTS
  All the six isolates of non-O1 non-O139 V. cholerae were identified by biochemical and serologic tests, and appeared to be β hemolytic. Twelve out of the 14 kinds of drugs showed 100% sensitive. All isolates were positive of ompW gene by real-time PCR, but negative for ctx, tcpA, zot, ace, rstR and gIII(CTX). Five of the six isolates were positive for toxR and hlyA, except for strain 1001434446. All strains had different PFGE types, but two strains had similar types. All strains had a low similarity compared to the toxigenic V. cholerae.
  
  
  CONCLUSIONS
  Six cases of non-O1 and non-O139 nontoxigenic V. cholerae infection appeared in the same period. Along with epidemiological information, we noticed that these cases had a sporadic nature, but frequently appeared in the same area. We got the impression that public health measurements should be strengthened, with special attention paid to those diarrhea outbreaks caused by non-O1/non-O139 strains since V. cholerae had appeared in low incidence.
authors: Maanshan City Center for Disease Control and Prevention, Maanshan 243000, China.
authors: Dao-li
authors: DL
authors: Chen
authors: Ping
authors: P
authors: Zhang
authors: Duo-chun
authors: DC
authors: Wang
authors: Jin
authors: J
authors: Chen
authors: Bai-qi
authors: BQ
authors: Yu
authors: Xian-feng
authors: XF
authors: Cheng
authors: Bao-wei
authors: BW
authors: Diao
authors: Hai-jian
authors: HJ
authors: Zhou
authors: Ming
authors: M
authors: Zhu
authors: Wan-fu
authors: WF
authors: Hu
authors: Sheng-wei
authors: SW
authors: Zhan
authors: Huai-qi
authors: HQ
authors: Jing
authors: Biao
authors: B
authors: Kan
mesh_terms: D000328:Adult; D000368:Aged; D002771:Cholera / Q000453:epidemiology* / Q000382:microbiology; D002772:Cholera Toxin / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D014734:Vibrio cholerae / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity
chemical_list: D002772:Cholera Toxin