title: Keratinocyte growth factor phage model peptides can promote human oral mucosal epithelial cell proliferation.
abstract: OBJECTIVE
  The objective of this study was to find keratinocyte growth factor (KGF) mimic peptides by a phage display library screening and to analyze their effects on proliferation of human oral mucosal epithelial cells (HOMECs).
  
  
  METHODS
  A phage display library was screened by anti-KGF antibody. ELISA was performed to select monoclonal phages with higher binding activity. The promotion of the phage model peptides on HOMEC proliferation were analyzed by MTT and their cell affinities were confirmed by immunofluorescence assay. Their effect on KGFR, human beta-defensin 3, c-Fos, and c-Jun in HOMEC were analyzed by quantitative real-time PCR.
  
  
  RESULTS
  Two model peptides with higher affinity with HOMEC were found to have promotive activity on cell proliferation, similar to that of KGF. These 2 model peptides have no KGF-like promotion effect on the expression of c-Fos and c-Jun.
  
  
  CONCLUSIONS
  The 2 phage model peptides can promote the proliferation of HOMEC in vitro without tumorigenic effects, which suggests their possible usages in oral mucosal wound healing.
authors: Shandong Provincial Key Laboratory of Oral Biomedicine, Ji'nan, PR China.
authors: Guo-Jv
authors: GJ
authors: Li
authors: Du-Yin
authors: DY
authors: Jiang
authors: Xi'an-lei
authors: XL
authors: Zong
authors: Xin
authors: X
authors: Xu
mesh_terms: D001435:Bacteriophages / Q000302:isolation & purification; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects; D051523:Fibroblast Growth Factor 7 / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects; D018716:Molecular Mimicry; D009061:Mouth Mucosa / Q000166:cytology / Q000187:drug effects*; D019151:Peptide Library; D016760:Proto-Oncogene Proteins c-fos / Q000187:drug effects; D016755:Proto-Oncogene Proteins c-jun / Q000187:drug effects; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000187:drug effects; D017422:Sequence Analysis, DNA; D023083:beta-Defensins / Q000187:drug effects
chemical_list: C422950:DEFB103A protein, human; D019151:Peptide Library; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D023083:beta-Defensins; D051523:Fibroblast Growth Factor 7; D051497:Receptor, Fibroblast Growth Factor, Type 2; C065104:keratinocyte growth factor receptor