title: Effect of fasting on energy metabolism and tenderizing enzymes in chicken breast muscle early postmortem.
abstract: Pre-slaughter fasting is a very important practice in the meat industry. The present study was designed to investigate the effect of fasting on energy metabolism and tenderizing enzymes in chicken muscle early postmortem. A total of 30 Yellow-feathered chickens were deprived of feed for 0 h, 12 h and 24 h before slaughter (n=10 each group). Breast muscles were removed and cut into 3 parts and stored at 0°C for 0 h, 3 h and 10 h. Samples were used for analyses of zymography, cathepsins, pH, glycogen/ATP/ADP/AMP, hormones and ultrastructure. Fasting caused the accelerated depletion (p<0.05) of glycogen, ATP and ADP before or immediately after slaughter, but no difference existed in ATP at 3 and 10 h (p>0.05). Fasting resulted in greater ultimate pH (p<0.05). Zymography indicated that fasting delayed the activation of μ/m-calpain (p<0.05), however, it accelerated the release of lysosomal enzymes (p<0.05). Fasting for 24 h resulted in greater ultrastructural changes and plasma corticosterone levels than fasting for 12 h and control groups. Therefore, fasting for no more than 12 h is acceptable in practice.
authors: Key Laboratory of Meat Processing and Quality Control, MOE, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, PR China.
authors: Sidang
authors: S
authors: Wang
authors: Chunbao
authors: C
authors: Li
authors: Xinglian
authors: X
authors: Xu
authors: Guanghong
authors: G
authors: Zhou
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002154:Calpain / Q000378:metabolism; D002645:Chickens; D003345:Corticosterone / Q000097:blood; D004734:Energy Metabolism / Q000502:physiology*; D004789:Enzyme Activation; D005215:Fasting; D005508:Food Deprivation; D006003:Glycogen / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000378:metabolism; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D011180:Postmortem Changes
chemical_list: D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D006003:Glycogen; D002154:Calpain; C449002:mu-calpain; D003345:Corticosterone