title: Microsatellite typing of Aspergillus flavus in patients with various clinical presentations of aspergillosis.
abstract: Aspergillus flavus is the second most important Aspergillus species associated with aspergillosis and the incidence of infections caused by it are increasing in the immunocompromised population. This species is of major epidemiological importance in regions with a dry and hot climate. Despite the growing clinical significance of A. flavus, data on its molecular epidemiology are scarce. This study was aimed at examining whether isolates from distinct genotypes were involved in distinct clinical forms of aspergillosis. Sixty-three clinical isolates of A. flavus recovered from 35 patients with various clinical presentations of aspergillosis were characterized by microsatellite typing. The highest discriminatory power for a single locus was obtained with the AFLA1 marker, which had 14 distinct alleles and a 0.903 D value. The combination of all six markers yielded 48 different genotypes with a 0.994 D value. There was a considerable genetic diversity in the isolates and patients with invasive aspergillosis were usually colonized by multiples genotypes. There was no evidence that a given genotype was associated with a particular clinical presentation of A. flavus aspergillosis. The occurrence of more than one genotype in clinical samples indicates that a patient may be infected by multiple genotypes and that any particular isolate from a clinical specimen may not necessarily be the one causing aspergillosis.
authors: Laboratoire de biologie moléculaire parasitaire et fongique, Faculté de Médecine de Sfax, Sfax, Tunisia.
authors: Inès
authors: I
authors: Hadrich
authors: Sourour
authors: S
authors: Neji
authors: Inès
authors: I
authors: Drira
authors: Houwaida
authors: H
authors: Trabelsi
authors: Nedia
authors: N
authors: Mahfoud
authors: Stéphane
authors: S
authors: Ranque
authors: Fattouma
authors: F
authors: Makni
authors: Ali
authors: A
authors: Ayadi
mesh_terms: D001228:Aspergillosis / Q000453:epidemiology / Q000382:microbiology*; D001231:Aspergillus flavus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D058889:Molecular Typing; D016533:Mycological Typing Techniques; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal