title: (1)H NMR-based metabolomics exploring biomarkers in rat cerebrospinal fluid after cerebral ischemia/reperfusion.
abstract: In our study, metabolomics was used to investigate biochemical changes in the early stages of rats focal cerebral ischemia/reperfusion (I/R) injury. Cerebrospinal fluid (CSF) samples at 0, 0.5, 1, 3, and 6 h of reperfusion (n = 10), based on (1)H NMR spectroscopy and multivariate data analyses, were tested to analyze the changing of metabolites during the early disease process. Partial least squares-discriminant analysis scores plots of the (1)H NMR data revealed clear differences among the experiment groups. Combining the results of the loading plot and t-test, we found that twenty-seven metabolites were changed significantly (p < 0.05) in the CSF samples among the different groups. Among that, the potential biomarkers in CSF of ischemic rats were: acetic acid, 3-hydroxyisovaleric acid, 3-hydroxybutyric acid, choline, l-alanine, creatine, creatinine, glycine, pyruvic acid, glycerol, glutamic acid, d-fructose, l-lactic acid and acetone. These findings help us understand the biochemical metabolite changes in CSF of I/R rats in early stages. What's more, metabolomics may, therefore, have the potential to be developed into a clinically useful diagnostic tool of ischemic brain injury.
authors: Jiangsu Key Laboratory of Anaesthesia, XuZhou Medical College, XuZhou, JiangSu, China.
authors: Yun
authors: Y
authors: Wang
authors: Yigang
authors: Y
authors: Wang
authors: Mei
authors: M
authors: Li
authors: Pengcheng
authors: P
authors: Xu
authors: Tao
authors: T
authors: Gu
authors: Tengfei
authors: T
authors: Ma
authors: Shuling
authors: S
authors: Gu
mesh_terms: D000596:Amino Acids / Q000134:cerebrospinal fluid; D000818:Animals; D015415:Biomarkers / Q000134:cerebrospinal fluid; D002545:Brain Ischemia / Q000134:cerebrospinal fluid* / Q000209:etiology; D016002:Discriminant Analysis; D020244:Infarction, Middle Cerebral Artery / Q000134:cerebrospinal fluid* / Q000150:complications; D016018:Least-Squares Analysis; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055432:Metabolomics; D015999:Multivariate Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000134:cerebrospinal fluid* / Q000209:etiology
chemical_list: D000596:Amino Acids; D015415:Biomarkers