title: Development of a rifampicin-resistant Bacillus subtilis strain for natto-fermentation showing enhanced exoenzyme production.
abstract: The ability to produce exoenzymes of a Bacillus subtilis natto starter strain was improved through selection of a rifampicin-resistant phenotype. Proteomic and zymographic analyses showed increased production of cellulolytic and proteolytic enzymes and decreased production of levansucrase. This mutant had a mutation (S487L) in the β-subunit of the RNA polymerase.
authors: Industrial Technology Institute of Ibaraki Prefecture, 3781-1 Nagaoka, Ibarakimachi, Ibaraki 311-3195, Japan.
authors: Yuji
authors: Y
authors: Kubo
authors: Takashi
authors: T
authors: Inaoka
authors: Tsuyoshi
authors: T
authors: Hachiya
authors: Masayuki
authors: M
authors: Miyake
authors: Sumitaka
authors: S
authors: Hase
authors: Rikio
authors: R
authors: Nakagawa
authors: Hiromasa
authors: H
authors: Hasegawa
authors: Kazumi
authors: K
authors: Funane
authors: Yasubumi
authors: Y
authors: Sakakibara
authors: Keitarou
authors: K
authors: Kimura
mesh_terms: D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D044602:Cellulases / Q000096:biosynthesis*; D024881:Drug Resistance, Bacterial; D005110:Extracellular Space / Q000201:enzymology; D005285:Fermentation; D010447:Peptide Hydrolases / Q000096:biosynthesis*; D010641:Phenotype; D012293:Rifampin / Q000494:pharmacology*; D045730:Soy Foods
chemical_list: D000904:Antibiotics, Antitubercular; D044602:Cellulases; D010447:Peptide Hydrolases; D012293:Rifampin