title: Rapid and specific electrochemical detection of prostate cancer cells using an aperture sensor array.
abstract: A rapid, simple and specific cancer cell counting sensor would allow for early detection and better disease management. We have developed a novel cell counting device that can specifically count 125 prostate cancer cells in both complex media with serum and a mixed cell population containing non-target cells within 15 min. The microfabricated glass chip with exposed gold apertures utilizes the anti-EpCAM antibody to selectively count prostate cancer cells via differential pulse voltammetry. The newly developed sensor exhibits excellent sensitivity and selectivity. The cells remain viable throughout the counting process and can be used for further analysis. This device could have utility for future applications in early stage cancer diagnosis.
authors: Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada.
authors: Mario
authors: M
authors: Moscovici
authors: Alyajahan
authors: A
authors: Bhimji
authors: Shana O
authors: SO
authors: Kelley
mesh_terms: D000906:Antibodies / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology / Q000378:metabolism; D015374:Biosensing Techniques; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000276:immunology / Q000378:metabolism; D045744:Cell Line, Tumor; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D000071858:Epithelial Cell Adhesion Molecule; D005292:Ferricyanides / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009360:Neoplastic Cells, Circulating / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D010084:Oxidation-Reduction; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology
chemical_list: D000906:Antibodies; D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule; D005292:Ferricyanides; C007931:hexacyanoferrate III; D006046:Gold