title: Attenuated mesangial cell proliferation related to store-operated Ca2+ entry in aged rat: the role of STIM 1 and Orai 1.
abstract: Store-operated Ca(2+) entry (SOCE) is a common and ubiquitous mechanism regulating Ca(2+) influx into cells and participates in numerous biological processes including cell proliferation. Glomerular mesangial cells (GMCs) play a role in the regulation of the glomerular filtration rate. From a clinical point of view, many physiological functions alter with age. In the present study, we used angiotensin II, glucagon, and the sarco/endoplasmic reticulum membrane Ca(2+) pump inhibitor thapsigargin to deplete the internal Ca(2+) stores for the activation of SOCE. We found that SOCE was significantly attenuated in GMCs from aged (22-month-old) rats. The expression of SOCE-related components, stromal interaction molecule 1 (STIM 1) and Orai 1, in freshly isolated glomeruli notably decreased, and STIM 1 and Orai 1 puncta formation significantly reduced in primary-cultured GMCs in aged rats. Moreover, specific knockdown of STIM 1 and Orai 1 by small interfering RNA markedly suppressed SOCE and cell proliferation of GMCs isolated from young (3-month-old) rats. We conclude that the attenuation of GMCs proliferation can be attributed to the decreased SOCE partially caused by reduced expression of STIM 1 and Orai 1.
authors: Department of Physiology, Anhui Medical University, Hefei, China, shenbing@ahmu.edu.cn.
authors: Bing
authors: B
authors: Shen
authors: Jinhang
authors: J
authors: Zhu
authors: Jin
authors: J
authors: Zhang
authors: Feifei
authors: F
authors: Jiang
authors: Zhaoyi
authors: Z
authors: Wang
authors: Yang
authors: Y
authors: Zhang
authors: Jie
authors: J
authors: Li
authors: Dake
authors: D
authors: Huang
authors: Daoping
authors: D
authors: Ke
authors: Rong
authors: R
authors: Ma
authors: Juan
authors: J
authors: Du
mesh_terms: D000375:Aging / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000096:biosynthesis / Q000235:genetics*; D002460:Cell Line; D049109:Cell Proliferation; D018507:Gene Expression Regulation, Developmental; D007678:Kidney Glomerulus / Q000166:cytology / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics*; D050527:Mesangial Cells / Q000166:cytology* / Q000378:metabolism; D000071740:ORAI1 Protein; D012313:RNA / Q000235:genetics*; D051381:Rats; D000071737:Stromal Interaction Molecule 1
chemical_list: D015220:Calcium Channels; D008562:Membrane Glycoproteins; D000071740:ORAI1 Protein; C532493:Orai1 protein, rat; C518857:Stim1 protein, rat; D000071737:Stromal Interaction Molecule 1; D012313:RNA; D002118:Calcium