title: Interactions between sulfated polysaccharides from sea brown algae and Toll-like receptors on HEK293 eukaryotic cells in vitro.
abstract: We studied the interactions between sulfated polysaccharides, fucoidans from sea brown algae Laminaria japonica, Laminaria cichorioides, and Fucus evanescens, with human Toll-like receptors (TLR) expressed on membranes of cultured human embryonic kidney cells (HEK293-null, HEK293-TLR2/CD14, HEK293-hTLR4/CD14-MD2, and HEK293-hTLR5). Fucoidans interacted with TLR-2 and TLR-4, but not with TLR-5, and were nontoxic for the cell cultures. L. japonica fucoidan (1 mg/ml), L. cichorioides fucoidan (100 μg/ml and 1 mg/ml), and F. evanescens fucoidan (10 μg/ml-1 mg/ml) activated transcription nuclear factor NF-ϰB by binding specifically to TLR-2. L. japonica fucoidan (100 μg/ml and 1 mg/ml), L. cichorioides fucoidan (10 μg/ml-1 mg/ml), and F. evanescens fucoidan (1 μg/ml-1 mg/ml) activated NF-ϰB via binding to TLR-4. These results indicated that fucoidans could induce in vivo defense from pathogenic microorganisms of various classes.
authors: Institute of Epidemiology and Microbiology, Siberian Division of the Russian Academy of Medical Sciences, Vladivostok, Russia. ilona_m@mail.ru
authors: I D
authors: ID
authors: Makarenkova
authors: D Yu
authors: DY
authors: Logunov
authors: A I
authors: AI
authors: Tukhvatulin
authors: I B
authors: IB
authors: Semenova
authors: N N
authors: NN
authors: Besednova
authors: T N
authors: TN
authors: Zvyagintseva
mesh_terms: D057809:HEK293 Cells; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism; D000459:Phaeophyceae / Q000737:chemistry*; D011134:Polysaccharides / Q000378:metabolism* / Q000494:pharmacology; D051195:Toll-Like Receptor 2 / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000378:metabolism; D051198:Toll-Like Receptor 5 / Q000378:metabolism; D051193:Toll-Like Receptors / Q000378:metabolism*
chemical_list: D016328:NF-kappa B; D011134:Polysaccharides; C495345:TLR4 protein, human; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051198:Toll-Like Receptor 5; D051193:Toll-Like Receptors; C007789:fucoidan