title: Animal model for cutaneous leishmaniasis.
abstract: Using cutaneous leishmaniasis of mice, the existence of so-called T helper (Th) cells type 1 and type 2 had been identified more than 20 years ago. Nowadays, it is well accepted that additional T cell populations as well as B cell-mediated immunity is required for immunity against Leishmania major. Finally, using inbred mouse strains, the relevance of genetical factors that influence anti-pathogen immunity as well as elements of the skin-immune system have been identified. This protocol describes a model for murine experimental leishmaniasis that tries to mimic natural parasite transmission by several means: (1) utilization of only infectious-stage parasites that are found in sand fly saliva, (2) intradermal inoculation, and (3) infection with only 1,000 parasites similar to the numbers inoculated by an infected sand fly.
authors: Department of Dermatology, University Medical Center, Johannes Gutenberg-University, Mainz, Germany.
authors: Michael R
authors: MR
authors: Fischer
authors: Dominik
authors: D
authors: John
authors: Kordula
authors: K
authors: Kautz-Neu
authors: Anja I
authors: AI
authors: Schermann
authors: Kirsten
authors: K
authors: Schwonberg
authors: Esther
authors: E
authors: von Stebut
mesh_terms: D000818:Animals; D007109:Immunity; D018320:Leishmania major / Q000502:physiology* / Q000648:ultrastructure; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology* / Q000473:pathology / Q000635:transmission*; D051379:Mice; D023421:Models, Animal; D011576:Psychodidae / Q000469:parasitology; D012867:Skin / Q000469:parasitology* / Q000473:pathology*; D013154:Spleen / Q000469:parasitology / Q000473:pathology