title: Quantitative LSPR imaging for biosensing with single nanostructure resolution.
abstract: Localized surface plasmon resonance (LSPR) imaging has the potential to map complex spatio-temporal variations in analyte concentration, such as those produced by protein secretions from live cells. A fundamental roadblock to the realization of such applications is the challenge of calibrating a nanoscale sensor for quantitative analysis. Here, we introduce a new, to our knowledge, LSPR imaging and analysis technique that enables the calibration of hundreds of individual gold nanostructures in parallel. The calibration allowed us to map the fractional occupancy of surface-bound receptors at individual nanostructures with nanomolar sensitivity and a temporal resolution of 225 ms. As a demonstration of the technique's applicability to molecular and cell biology, the calibrated array was used for the quantitative LSPR imaging of anti-c-myc antibodies harvested from a cultured 9E10 hybridoma cell line without the need for further purification or processing.
authors: Naval Research Laboratory, Washington DC, USA. marc.raphael@nrl.navy.mil
authors: Marc P
authors: MP
authors: Raphael
authors: Joseph A
authors: JA
authors: Christodoulides
authors: James B
authors: JB
authors: Delehanty
authors: James P
authors: JP
authors: Long
authors: Pehr E
authors: PE
authors: Pehrsson
authors: Jeff M
authors: JM
authors: Byers
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D051379:Mice; D049329:Nanostructures / Q000737:chemistry*; D016271:Proto-Oncogene Proteins c-myc / Q000276:immunology; D013057:Spectrum Analysis; D020349:Surface Plasmon Resonance / Q000379:methods*; D013997:Time Factors
chemical_list: D000906:Antibodies; D016271:Proto-Oncogene Proteins c-myc; D006046:Gold