title: Backbone ¹H, ¹³C, ¹⁵N NMR assignments of yeast OMP synthase in unliganded form and in complex with orotidine 5'-monophosphate.
abstract: The type I phosphoribosyltransferase OMP synthase (EC 2.4.2.10) is involved in de novo synthesis of pyrimidine nucleotides forming the UMP precursor orotidine 5'-monophosphate (OMP). The homodimeric enzyme has a Rossman α/β core topped by a base-enclosing "hood" domain and a flexible domain-swapped catalytic loop. High-resolution X-ray structures of the homologous Salmonella typhimurium and yeast enzymes show that a general compacting of the core as well as movement of the hood and a major disorder-to-order transition of the loop occur upon binding of ligands MgPRPP and orotate. Here we present backbone NMR assignments for the unliganded yeast enzyme (49 kDa) and its complex with product OMP. We were able to assign 212-213 of the 225 non-proline backbone (15)N and amide proton resonances. Significant difference in chemical shifts of the amide cross peaks occur in regions of the structure that undergo movement upon ligand occupancy in the S. typhimurium enzyme.
authors: Department of Biochemistry, Temple University School of Medicine, 3307 N Broad St., Philadelphia, PA, 19140, USA.
authors: Michael Riis
authors: MR
authors: Hansen
authors: Richard
authors: R
authors: Harris
authors: Eric W
authors: EW
authors: Barr
authors: Hong
authors: H
authors: Cheng
authors: Mark E
authors: ME
authors: Girvin
authors: Charles
authors: C
authors: Grubmeyer
mesh_terms: D000595:Amino Acid Sequence; D055162:Biocatalysis; D002247:Carbon Isotopes; D006859:Hydrogen; D008024:Ligands; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D009962:Orotate Phosphoribosyltransferase / Q000737:chemistry*; D017433:Protein Structure, Secondary; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000201:enzymology*; D014542:Uridine Monophosphate / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism
chemical_list: D002247:Carbon Isotopes; D008024:Ligands; D009587:Nitrogen Isotopes; C005417:orotidylic acid; D006859:Hydrogen; D014542:Uridine Monophosphate; D009962:Orotate Phosphoribosyltransferase