title: Multiplexed colorimetric detection of Kaposi's sarcoma associated herpesvirus and Bartonella DNA using gold and silver nanoparticles.
abstract: Kaposi's sarcoma (KS) is an infectious cancer occurring most commonly in human immunodeficiency virus (HIV) positive patients and in endemic regions, such as Sub-Saharan Africa, where KS is among the top four most prevalent cancers. The cause of KS is the Kaposi's sarcoma-associated herpesvirus (KSHV, also called HHV-8), an oncogenic herpesvirus that while routinely diagnosed in developed nations, provides challenges to developing world medical providers and point-of-care detection. A major challenge in the diagnosis of KS is the existence of a number of other diseases with similar clinical presentation and histopathological features, requiring the detection of KSHV in a biopsy sample. In this work we develop an answer to this challenge by creating a multiplexed one-pot detection system for KSHV DNA and DNA from a frequently confounding disease, bacillary angiomatosis. Gold and silver nanoparticle aggregation reactions are tuned for each target and a multi-color change system is developed capable of detecting both targets down to levels between 1 nM and 2 nM. The system developed here could later be integrated with microfluidic sample processing to create a final device capable of solving the two major challenges in point-of-care KS detection.
authors: Department of Biomedical Engineering, Cornell University, Ithaca, NY, USA.
authors: Matthew
authors: M
authors: Mancuso
authors: Li
authors: L
authors: Jiang
authors: Ethel
authors: E
authors: Cesarman
authors: David
authors: D
authors: Erickson
mesh_terms: D001473:Bartonella / Q000235:genetics* / Q000302:isolation & purification; D003124:Colorimetry / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D006046:Gold; D019288:Herpesvirus 8, Human / Q000235:genetics* / Q000302:isolation & purification; D053768:Metal Nanoparticles; D012834:Silver
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral; D012834:Silver; D006046:Gold