title: The association between development and progression of multinodular goiter and thyroid-stimulating hormone receptor gene D727E and P52T polymorphisms.
abstract: OBJECTIVE
  This study has been performed on a Turkish population with multinodular goiter (MNG) to investigate the thyroid-stimulating hormone receptor (TSHR) gene D727E and P52T polymorphisms.
  
  
  METHODS
  DNA samples were isolated from 300 patients with MNG and 142 controls. Polymerase chain reaction-restriction fragment length polymorphism and agarose gel electrophoresis were used.
  
  
  RESULTS
  The D727E polymorphism G-allele frequency and the CG and GG genotypes were significantly higher in patients with MNG. However, there was no significant difference in the P52T polymorphism between patients and control subjects.
  
  
  CONCLUSIONS
  As a conclusion, the D727E polymorphism G allele may be related to MNG development in the studied population.
authors: Department of Biology, Faculty of Arts and Science, Mus Alparslan University, Turkey. bayrambanu@hotmail.com
authors: Banu
authors: B
authors: Bayram
authors: Resit
authors: R
authors: Sonmez
authors: Sedat
authors: S
authors: Bozari
authors: Harun
authors: H
authors: Onlu
authors: Züleyha
authors: Z
authors: Turkoglu
authors: Fezan Sahin
authors: FS
authors: Mutlu
mesh_terms: D000483:Alleles; D018450:Disease Progression; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006044:Goiter, Nodular / Q000235:genetics* / Q000503:physiopathology*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011989:Receptors, Thyrotropin / Q000235:genetics*; D014421:Turkey
chemical_list: D011989:Receptors, Thyrotropin