title: Lipidomics identification of metabolic biomarkers in chemically induced hypertriglyceridemic mice.
abstract: In this study, we aim to identify the potential biomarkers in hTG pathogenesis in schisandrin B-induced hTG mouse model. To investigate whether these identified biomarkers are only specific to schisandrin B-induced hTG mouse model, we also measured these biomarkers in a high fat diet (HFD)-induced hTG mouse model. We employed a LC/MS/MS-based lipidomic approach for the study. Mouse liver and serum metabolites were separated by reversed phase liquid chromatography. Metabolite candidates were identified by matching with marker retention times, isotope distribution patterns, and high-resolution MS/MS fragmentation patterns. Subsequently, target candidates were quantified by quantitative MS. In the schisandrin B-induced hTG mice, we found that the plasma fatty acids, diglyceroids, and phospholipids were significantly increased. Palmitic acid and stearic acid were increased in the plasma; oleic acid, linoleic acid, linolenic acid, arachidonic acid, and docosahexaenoic acid were increased in both the plasma and the liver. Acetyl-CoA, malonyl-CoA, and succinyl-CoA were increased only in the liver. The changes in levels of these identified markers were also observed in HFD-induced hTG mouse model. The consistent results obtained from both hTG models not only suggest novel biomarkers in hTG pathogenesis, but they also provide insight into the underlying mechanism of the schisandrin B-induced hTG.
authors: Centre for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
authors: Hiu Yee
authors: HY
authors: Kwan
authors: Yong-Mei
authors: YM
authors: Hu
authors: Chi Leung
authors: CL
authors: Chan
authors: Hui-Hui
authors: HH
authors: Cao
authors: Chi Yan
authors: CY
authors: Cheng
authors: Si-Yuan
authors: SY
authors: Pan
authors: Kai Wing
authors: KW
authors: Tse
authors: Yiu Cheong
authors: YC
authors: Wu
authors: Zhi-Ling
authors: ZL
authors: Yu
authors: Wang Fun
authors: WF
authors: Fong
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D002138:Calibration; D002853:Chromatography, Liquid; D034242:Cyclooctanes / Q000494:pharmacology; D015228:Hypertriglyceridemia / Q000139:chemically induced / Q000378:metabolism*; D017705:Lignans / Q000494:pharmacology; D008055:Lipids; D051379:Mice; D011083:Polycyclic Compounds / Q000494:pharmacology; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D014280:Triglycerides / Q000097:blood
chemical_list: D015415:Biomarkers; D034242:Cyclooctanes; D017705:Lignans; D008055:Lipids; D011083:Polycyclic Compounds; D014280:Triglycerides; C015499:schizandrin B