title: Effect of sodium hypochlorite and EDTA irrigation, individually and in alternation, on dentin microhardness at the furcation area of mandibular molars.
abstract: The aim of this study was to evaluate the effect of irrigation regimens on dentin microhardness at the furcation area of mandibular molars, using sodium hypochlorite and ethylenediaminetetraacetic acid (EDTA), individually and in alternation. The occlusal surface and the roots of 20 non-carious extracted human permanent mandibular molars were cut transversally and discarded. The tooth blocks were embedded in acrylic resin and randomly assigned to 4 groups (n=5) according to the irrigating regimens: 1% NaOCl solution, 17% EDTA solution, 1% NaOCl and 17% EDTA and distilled water (control). Knoop microhardness of dentin at the furcation area was evaluated. Data were analyzed using one-way ANOVA and Tukey's multiple comparison tests (α=0.05). The results of this study indicated that all irrigation solutions, except for distilled water (control), decreased dentin microhardness. EDTA did not show a significant difference with NaOCl/EDTA (p>0.05), but showed a significant difference with NaOCl (p<0.01). EDTA and NaOCl/EDTA showed a maximum decrease in microhardness. The 17% EDTA solution, either alone or in combination with 1% NaOCl reduced significantly dentin microhardness at the furcation area of mandibular molars.
authors: Department of Restorative Dentistry, Ribeirão Preto Dental School, USP - University of São Paulo, Ribeirão Preto, SP, Brazil.
authors: Danilo
authors: D
authors: Zaparolli
authors: Paulo César
authors: PC
authors: Saquy
authors: Antonio Miranda
authors: AM
authors: Cruz-Filho
mesh_terms: D003786:Dental Pulp Cavity / Q000187:drug effects*; D003799:Dental Stress Analysis / Q000295:instrumentation; D003804:Dentin / Q000187:drug effects*; D004492:Edetic Acid / Q000494:pharmacology*; D006244:Hardness; D006801:Humans; D008334:Mandible; D008422:Materials Testing; D008963:Molar / Q000187:drug effects*; D012388:Root Canal Irrigants / Q000494:pharmacology*; D018915:Root Canal Preparation / Q000379:methods; D012973:Sodium Hypochlorite / Q000494:pharmacology*; D014092:Tooth Root / Q000187:drug effects
chemical_list: D012388:Root Canal Irrigants; D004492:Edetic Acid; D012973:Sodium Hypochlorite