title: Cyclophilin A-dependent restriction to capsid N121K mutant human immunodeficiency virus type 1 in a broad range of cell lines.
abstract: Human immunodeficiency virus type 1 (HIV-1) infection of most human cells is dependent on cyclophilin A (CypA); however, the opposite phenomenon, known as CypA-dependent inhibition, is also observed in the combination of some capsid (CA) mutations and cell lines. Here, we identified a CA N121K mutant whose infection of 293T, Jurkat, and HeLa cells was impaired by CypA. The N121K mutant could be a useful tool for analyzing the mechanisms underlying CypA-dependent restriction.
authors: AIDS Research Center, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan. taichiro@nih.go.jp
authors: Taichiro
authors: T
authors: Takemura
authors: Miyako
authors: M
authors: Kawamata
authors: Miho
authors: M
authors: Urabe
authors: Tsutomu
authors: T
authors: Murakami
mesh_terms: D036022:Capsid Proteins / Q000235:genetics*; D021984:Cyclophilin A / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D057809:HEK293 Cells; D016655:HIV Core Protein p24 / Q000235:genetics; D015658:HIV Infections / Q000378:metabolism*; D015497:HIV-1 / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D008156:Luciferases; D020125:Mutation, Missense / Q000235:genetics; D014779:Virus Replication / Q000235:genetics / Q000502:physiology*
chemical_list: D036022:Capsid Proteins; D016655:HIV Core Protein p24; D008156:Luciferases; D021984:Cyclophilin A