title: Differentiation of human endometrial stem cells into urothelial cells on a three-dimensional nanofibrous silk-collagen scaffold: an autologous cell resource for reconstruction of the urinary bladder wall.
abstract: Reconstruction of the bladder wall via in vitro differentiated stem cells on an appropriate scaffold could be used in such conditions as cancer and neurogenic urinary bladder. This study aimed to examine the potential of human endometrial stem cells (EnSCs) to form urinary bladder epithelial cells (urothelium) on nanofibrous silk-collagen scaffolds, for construction of the urinary bladder wall. After passage 4, EnSCs were induced by keratinocyte growth factor (KGF) and epidermal growth factor (EGF) and seeded on electrospun collagen-V, silk and silk-collagen nanofibres. Later we tested urothelium-specific genes and proteins (uroplakin-Ia, uroplakin-Ib, uroplakin-II, uroplakin-III and cytokeratin 20) by immunocytochemistry, RT-PCR and western blot analyses. Scanning electron microscopy (SEM) and histology were used to detect cell-matrix interactions. DMEM/F12 supplemented by KGF and EGF induced EnSCs to express urothelial cell-specific genes and proteins. Either collagen, silk or silk-collagen scaffolds promoted cell proliferation. The nanofibrous silk-collagen scaffolds provided a three-dimensional (3D) structure to maximize cell-matrix penetration and increase differentiation of the EnSCs. Human EnSCs seeded on 3D nanofibrous silk-collagen scaffolds and differentiated to urothelial cells provide a suitable source for potential use in bladder wall reconstruction in women.
authors: Tissue Engineering and Stem Cell Department, Research Centre for Science and Technology in Medicine (RCSTiM), Tehran University of Medical Sciences, Tehran, Iran.|Applied Cell Sciences Department, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors: Alireza
authors: A
authors: Shoae-Hassani
authors: Tissue Engineering and Stem Cell Department, Research Centre for Science and Technology in Medicine (RCSTiM), Tehran University of Medical Sciences, Tehran, Iran.|Proteomics Research Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors: Seyed Abdolreza
authors: SA
authors: Mortazavi-Tabatabaei
authors: Tissue Engineering and Stem Cell Department, Research Centre for Science and Technology in Medicine (RCSTiM), Tehran University of Medical Sciences, Tehran, Iran.|Tissue Engineering Department, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors: Shiva
authors: S
authors: Sharif
authors: UCL Centre for Nanotechnology & Regenerative Medicine, Division of Surgery & Interventional Science, University College London, London, United Kingdom.
authors: Alexander Marcus
authors: AM
authors: Seifalian
authors: Department of Oncology-Pathology, Karolinska University Hospital, Stockholm, Sweden.
authors: Alireza
authors: A
authors: Azimi
authors: Applied Cell Sciences Department, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.|Department of Chemical Engineering, Biological Systems Engineering Laboratory, Centre for Process Systems Engineering, Imperial College London, United Kingdom.
authors: Ali
authors: A
authors: Samadikuchaksaraei
authors: Tissue Engineering and Stem Cell Department, Research Centre for Science and Technology in Medicine (RCSTiM), Tehran University of Medical Sciences, Tehran, Iran.|Applied Cell Sciences Department, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.|Tissue Engineering Department, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors: Javad
authors: J
authors: Verdi
mesh_terms: D000328:Adult; D001672:Biocompatible Materials / Q000494:pharmacology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry; D004717:Endometrium / Q000166:cytology*; D004797:Enzyme-Linked Immunosorbent Assay; D004815:Epidermal Growth Factor / Q000378:metabolism; D005260:Female; D051523:Fibroblast Growth Factor 7 / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000166:cytology; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry; D010641:Phenotype; D047011:Silk / Q000737:chemistry; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D001743:Urinary Bladder / Q000473:pathology*; D019459:Urothelium / Q000166:cytology / Q000473:pathology*
chemical_list: D001672:Biocompatible Materials; D047011:Silk; D051523:Fibroblast Growth Factor 7; D004815:Epidermal Growth Factor; D003094:Collagen
keywords: biomarker; cytokeratin; differentiation; stem cell; uroplakin; urothelium