title: Standardization of pathological investigations in the framework of experimental ASFV infections.
abstract: African swine fever is still one of the major viral diseases of swine for which a commercial vaccine is lacking. For the design and development of such preventive products, researchers involved in African swine fever virus (ASFV) vaccinology need standardized challenge protocols and well characterized clinical, pathological and immunological responses of inbreed and outbreed pigs to different viral strains and vaccine-like products. The different approaches used should be assessed by immunologist, virologist and pathologist expertise. The main objectives of this guideline are to (1) briefly contextualize the clinical and pathological ASFV presentations focusing on points that are critical for pathogenesis, (2) provide recommendations concerning the analysis of clinical, gross and microscopic observations and (3) standardize the pathological report, the terminology employed and the evaluation of the severity of the lesions between the ASFV research groups for comparing inter-group data. The presented guidelines establish new approaches to integrate such relevant pathological data with virological and immunological testing, giving support to the global interpretation of the findings in the future experiments of ASFV-related vaccinology and immunology.
authors: Centre de Recerca en Sanitat Animal, UAB-IRTA, Edifici CReSA, Campus UAB, 08193 Bellaterra, Barcelona, Spain. ivan.galindo@worldpathol.com
authors: Iván
authors: I
authors: Galindo-Cardiel
authors: María
authors: M
authors: Ballester
authors: David
authors: D
authors: Solanes
authors: Miquel
authors: M
authors: Nofrarías
authors: Sergio
authors: S
authors: López-Soria
authors: Jordi M
authors: JM
authors: Argilaguet
authors: Anna
authors: A
authors: Lacasta
authors: Francesc
authors: F
authors: Accensi
authors: Fernando
authors: F
authors: Rodríguez
authors: Joaquim
authors: J
authors: Segalés
mesh_terms: D000357:African Swine Fever / Q000473:pathology*; D000358:African Swine Fever Virus / Q000472:pathogenicity*; D000818:Animals; D004195:Disease Models, Animal; D055808:Drug Discovery / Q000379:methods; D017408:Guidelines as Topic; D010336:Pathology / Q000379:methods* / Q000592:standards*; D013552:Swine; D014765:Viral Vaccines / Q000276:immunology / Q000302:isolation & purification
chemical_list: D014765:Viral Vaccines