title: Purification and characterization of ovotransferrin from Crocodylus siamensis.
abstract: Ovotransferrin (OTf) is the major glycoprotein in reptile egg whites. However, knowledge concerning its functional and biological properties remains limited. In this study, OTf from Crocodylus siamensis was purified and characterized. The proteins were precipitated with 80 % ammonium sulfate and then purified by anion exchange chromatography followed by hydrophobic interaction chromatography. The purified crocodile ovotransferrin (cOTf) had a molecular weight of 79 kDa. Analysis by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) indicated multiple isoforms of cOTf, which had isoelectric points ranging from 6.0 to 6.8. cOTf was N-linked glycosylated protein identified by using PNGase F deglycosylation technique. Optimal autoproteolysis of cOTf occurred under acidic conditions and pH values more than 5, which differs from that of OTf.
authors: Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Sukanya
authors: S
authors: Chaipayang
authors: Napus
authors: N
authors: Heamatorn
authors: Likhid
authors: L
authors: Keha
authors: Sakda
authors: S
authors: Daduang
authors: Chomphunuch
authors: C
authors: Songsiriritthigul
authors: Prasan
authors: P
authors: Swatsitang
authors: Apisak
authors: A
authors: Dhiravisit
authors: Sompong
authors: S
authors: Thammasirirak
mesh_terms: D000489:Alligators and Crocodiles; D000818:Animals; D003207:Conalbumin / Q000737:chemistry* / Q000302:isolation & purification*; D015180:Electrophoresis, Gel, Two-Dimensional; D007526:Isoelectric Point; D008970:Molecular Weight; D010063:Ovum / Q000737:chemistry; D030162:Reptilian Proteins / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D030162:Reptilian Proteins; D003207:Conalbumin