title: Purification and autolysis of the ficin isoforms from fig (Ficus carica cv. Sabz) latex.
abstract: Ficin (EC 3.4.22.3), a cysteine endoproteolytic protease in fig trees' latex, has multiple isoforms. Until now, no data on autolysis of individual ficins (ficin isoforms) are available. Following purification, ficins' autolysis was determined by HPLC chromatogram changes and ultrafiltrations at different temperatures and storage times. These results showed that the number of HPLC peaks in latex proteins purification of Ficus carica cv. Sabz varied from previous fig varieties or cultivars. Proteolytic activity of ficins was inhibited by specific cysteine protease inhibitors, confirming the participation of the cysteine residue in the active site. The zeta potential of the first two eluted peaks (I and II) was negative, while that of other peaks were positive. All ficins were susceptible to autolysis when stored at high temperatures. In contrast, only the last two ficins (B, C) were prone to autolysis at cold temperature after long storage period. The rate of degradation of the ficins was significantly increased with the increased storage time. The ficin (A) related to peak (III) had the highest and the lowest surface hydrophobic patches and ratio of autolytic to proteolytic activity, respectively.
authors: Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran.
authors: Hamid
authors: H
authors: Zare
authors: Ali Akbar
authors: AA
authors: Moosavi-Movahedi
authors: Maryam
authors: M
authors: Salami
authors: Morteza
authors: M
authors: Mirzaei
authors: Ali Akbar
authors: AA
authors: Saboury
authors: Nader
authors: N
authors: Sheibani
mesh_terms: D001329:Autolysis / Q000378:metabolism; D057056:Cysteine Proteases / Q000378:metabolism; D030681:Ficus / Q000378:metabolism*; D010940:Plant Proteins / Q000302:isolation & purification* / Q000378:metabolism*; D020033:Protein Isoforms / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D020033:Protein Isoforms; D057056:Cysteine Proteases