title: MicroRNA-182 plays an onco-miRNA role in cervical cancer.
abstract: OBJECTIVE
  The purposes of this study were to identify aberrantly expressed miRNAs and investigate their pathogenic roles in cervical cancer.
  
  
  METHODS
  miRNA expression was assessed in cervical cancer cell lines, micro-dissected normal cervical epithelium cells and primary cervical carcinoma by TaqMan RT-PCR. Spatial expression of miR-182 in cervical carcinoma and normal cervix was explored by in situ hybridization. HeLa xenograft mice model was used for evaluation of the effect on tumor growth of miR-182 inhibitor. Western blot, flow cytometry and gene expression analysis were used for identification of the functional role of miR-182 in HeLa cells.
  
  
  RESULTS
  Two up-regulated (miR-182 and -183) and nine down-regulated (miR-211, 145, 223, 150, 142-5p, 328, 195, 199b, 142-3p) microRNAs were consistently identified in cervical cancer cell lines. Further investigation confirmed the most up-regulated miRNA (miR-182) was significantly elevated in primary cervical carcinoma and discovered a significant correlation between the increased expression of miR-182 and advanced stages of cervical cancer. In HeLa xenograft mouse model, we demonstrated that inhibition of the miR-182 could exert the effect of tumor growth regression. Western blot, flow cytometry and pathway analysis for the HeLa cells with miR-182 over/down-expression in vitro showed that miR-182 was involved in apoptosis and cell cycle pathways, it also associated with the regulation of FOXO1.
  
  
  CONCLUSIONS
  Our findings indicated that miR-182 plays an onco-miRNA role in cervical cancer and its alteration is associated with cervical cancer pathogenesis by disrupting cell proliferation.
authors: Department of Obstetrics & Gynaecology, The Chinese University of Hong Kong, Hong Kong, China.
authors: Tao
authors: T
authors: Tang
authors: Hoi Kin
authors: HK
authors: Wong
authors: Wenyi
authors: W
authors: Gu
authors: Mei-yung
authors: MY
authors: Yu
authors: Ka-Fai
authors: KF
authors: To
authors: Chi Chiu
authors: CC
authors: Wang
authors: Yick Fu
authors: YF
authors: Wong
authors: Tak Hong
authors: TH
authors: Cheung
authors: Tony Kwok Hung
authors: TK
authors: Chung
authors: Kwong Wai
authors: KW
authors: Choy
mesh_terms: D000818:Animals; D017209:Apoptosis; D002453:Cell Cycle; D005260:Female; D005434:Flow Cytometry; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000235:genetics; D020869:Gene Expression Profiling; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000502:physiology*; D002583:Uterine Cervical Neoplasms / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
chemical_list: C085126:FOXO1 protein, human; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; D035683:MicroRNAs; C538984:Mirn182 microRNA, human