title: Optimizing nucleic acid extraction from thyroid fine-needle aspiration cells in stained slides, formalin-fixed/paraffin-embedded tissues, and long-term stored blood samples.
abstract: OBJECTIVE
  Adequate isolation of nucleic acids from peripheral blood, fine-needle aspiration cells in stained slides, and fresh and formalin-fixed/paraffin-embedded tissues is crucial to ensure the success of molecular endocrinology techniques, especially when samples are stored for long periods, or when no other samples can be collected from patients who are lost to follow-up. Here, we evaluate several procedures to improve current methodologies for DNA (salting-out) and RNA isolation.
  
  
  METHODS
  We used proteinase K treatment, heat shock, and other adaptations to increase the amount and quality of the material retrieved from the samples.
  
  
  RESULTS
  We successfully isolated DNA and RNA from the samples described above, and this material was suitable for PCR, methylation profiling, real-time PCR and DNA sequencing.
  
  
  CONCLUSIONS
  The techniques herein applied to isolate nucleic acids allowed further reliable molecular analyses.
authors: Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Sao Paulo, SP, Brazil.
authors: Marina M L
authors: MM
authors: Kizys
authors: Mirian G
authors: MG
authors: Cardoso
authors: Susan C
authors: SC
authors: Lindsey
authors: Michelle Y
authors: MY
authors: Harada
authors: Fernando A
authors: FA
authors: Soares
authors: Maria Clara C
authors: MC
authors: Melo
authors: Marlyn Z
authors: MZ
authors: Montoya
authors: Teresa S
authors: TS
authors: Kasamatsu
authors: Ilda S
authors: IS
authors: Kunii
authors: Gisele
authors: G
authors: Giannocco
authors: João Roberto M
authors: JR
authors: Martins
authors: Janete M
authors: JM
authors: Cerutti
authors: Rui M B
authors: RM
authors: Maciel
authors: Magnus R
authors: MR
authors: Dias-da-Silva
mesh_terms: D044963:Biopsy, Fine-Needle; D004247:DNA / Q000097:blood / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D012313:RNA / Q000097:blood / Q000302:isolation & purification*; D013194:Staining and Labeling; D013961:Thyroid Gland / Q000473:pathology; D013964:Thyroid Neoplasms / Q000235:genetics / Q000473:pathology; D016707:Tissue Fixation
chemical_list: D012313:RNA; D004247:DNA