title: Molecular diagnosis of autoimmune blistering diseases.
abstract: Autoimmune bullous diseases are the best-characterized autoimmune skin diseases. Molecular diagnosis of these diseases has become possible due to the identification of their target autoantigens over the past three decades. In this review, we summarize methodology for categorizing autoimmune bullous diseases by means of combinations of direct and indirect immunofluorescence techniques using normal human skin sections, rat bladder sections and COS7 cells transfected with desmocollins 1-3 encoded vectors, enzyme-linked immunosorbent assays and immunoblotting with normal human epidermal extracts, dermal extracts, purified proteins from cell cultures and recombinant proteins.
authors: Department of Dermatology, School of Medicine, and Institute of Cutaneous Cell Biology, Kurume University, Kurume, Fukuoka, Japan.
authors: Daisuke
authors: D
authors: Tsuruta
authors: Teruki
authors: T
authors: Dainichi
authors: Takahiro
authors: T
authors: Hamada
authors: Norito
authors: N
authors: Ishii
authors: Takashi
authors: T
authors: Hashimoto
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D001768:Blister / Q000175:diagnosis / Q000276:immunology / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012867:Skin / Q000276:immunology / Q000473:pathology*; D012872:Skin Diseases, Vesiculobullous / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology