title: [Isolation, charcaterization of an anthracene degrading bacterium Martelella sp. AD-3 and cloning of dioxygenase gene].
abstract: Anthracene, among the 16 US EPA polycyclic aromatic hydrocarbons (PAHs), is a typical low molecular weight environmental contaminant, which gains concern on its biodegradation under hypersaline condition. In this study, an anthracene-degrading bacterial strain was isolated from highly saline petroleum-contaminated soil. Based on its physiological, biochemical characteristics and 16S rDNA sequence analysis, the bacteria was preliminary identified and named as Martelella sp. AD-3. The strain was able to utilize anthracene as sole carbon source for growth and the degradation occurred under broad salinities (0.1% to 10%) and varying pHs (6.0 to 10.0). The optimized degradation conditions were initial concentration 25 mg x L(-1), culture temperature 30 degrees C, pH 9.0 and salinity 3%. And 94.6% of anthracene was degraded by strain AD-3 under the optimal conditions within 6 days. Degenerate primers design was performed with a reported dioxygenase alpha subunit homologous gene. A length of 307 bp fragment of the partial dioxygenase gene sequences (GenBank accession: JF823991.1) was amplified by nested PCR. The clones amino acid sequence from strain AD-3 showed 95% identity to that of the partial naphthalene dioxygenase large-subunit from Marinobacter sp. NCE312 (AF295033). The results lay a foundation for the further study of molecular mechanism involved in the PAHs biodegradation by strain AD-3.
authors: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China. cuichangzheng@ecust.edu.cn
authors: Chang-Zheng
authors: CZ
authors: Cui
authors: Tian-Cai
authors: TC
authors: Feng
authors: Ya-Qi
authors: YQ
authors: Yu
authors: Fei
authors: F
authors: Dong
authors: Xin-Mei
authors: XM
authors: Yang
authors: Yao-Yu
authors: YY
authors: Feng
authors: Yong-Di
authors: YD
authors: Liu
authors: Han-Ping
authors: HP
authors: Lin
mesh_terms: D020561:Alphaproteobacteria / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*; D000873:Anthracenes / Q000302:isolation & purification* / Q000378:metabolism; D001673:Biodegradation, Environmental; D003001:Cloning, Molecular; D049308:Dioxygenases / Q000235:genetics*; D004785:Environmental Pollutants / Q000302:isolation & purification* / Q000378:metabolism; D054712:Salinity; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D000873:Anthracenes; D004785:Environmental Pollutants; D012987:Soil; D049308:Dioxygenases; C034020:anthracene