title: Effect of trimethyllead chloride on slowly activating (SV) channels in red beet (Beta vulgaris L.) taproots.
abstract: The patch-clamp technique was used to examine the effect of trimethyllead chloride (Met(3)PbCl) on SV channel activity in red beet (Beta vulgaris L.) taproot vacuoles. It was found that in the control bath the macroscopic currents showed the typical slow activation and a strong outward rectification of the steady-state currents. An addition of Met(3)PbCl to the bath solution blocked, in a concentration-dependent manner, SV currents in red beet vacuoles. The time constant τ increased several times in the presence of 100 μM trimethyllead chloride at all voltages tested. When single channel properties were analyzed, only little channel activity could be recorded in the presence of 100 μM Met(3)PbCl. Trimethyllead chloride decreased significantly (by about one order of magnitude) the open probability of single channels. The recordings of single channel activity obtained in the presence and absence of Met(3)PbCl showed that organolead only slightly (by ca. 10%) decreased the unitary conductance of single channels. It was also found that Met(3)PbCl diminished significantly the number of SV channel openings, whereas it did not change the opening times of the channels. Taken together, these results suggest that Met(3)PbCl binding site is located outside the channel selectivity filter.
authors: Department of Physics and Biophysics, Wrocław University of Environmental and Life Sciences, Norwida 25, PL-50-375 Wrocław, Poland.
authors: Zenon
authors: Z
authors: Trela
authors: Zbigniew
authors: Z
authors: Burdach
authors: Stanisław
authors: S
authors: Przestalski
authors: Waldemar
authors: W
authors: Karcz
mesh_terms: D027461:Beta vulgaris / Q000187:drug effects / Q000502:physiology*; D002712:Chlorides / Q000633:toxicity*; D055724:Electrophysiological Phenomena; D004785:Environmental Pollutants / Q000633:toxicity*; D007473:Ion Channels / Q000037:antagonists & inhibitors* / Q000502:physiology; D018408:Patch-Clamp Techniques; D018517:Plant Roots / Q000648:ultrastructure*; D013756:Tetraethyl Lead / Q000031:analogs & derivatives* / Q000633:toxicity; D014617:Vacuoles / Q000737:chemistry
chemical_list: D002712:Chlorides; D004785:Environmental Pollutants; D007473:Ion Channels; D013756:Tetraethyl Lead; C039474:trimethyllead