title: ALDH1 expression indicates chemotherapy resistance and poor outcome in node-negative rectal cancer.
abstract: The cancer stem cell marker aldehyde dehydrogenase 1 (ALDH1) associates with treatment resistance and adverse outcome in several human cancers. We studied ALDH1 expression in rectal cancer, with special emphasis on its association with treatment response and disease outcome. Immunohistochemical staining for ALDH1 was conducted for 64 biopsies and 209 operative samples from rectal cancer patients treated with short- (n = 89) or long-course (n = 46) (chemo)radiotherapy plus surgery, or with surgery only (n = 74). The staining results were compared to clinicopathological variables, tumor regression grade (TRG) and disease outcome. Nuclear β-catenin expression pattern was analyzed from 197 operative samples. Positive ALDH1 expression was present in 149 operative samples (71%), correlating with deficient nuclear β-catenin regulation (P = .018). In a pairwise comparison of respective biopsy and operative samples, ALDH1 expression remained stable or increased after preoperative (chemo)radiotherapy in most of the cases, while it decreased in few cases only (P = .02 for positive/negative category; P <.001 for intensity). ALDH1 expression did not, however, relate to tumor regression grade. In node-negative rectal cancer, ALDH1 expression was an independent predictor of short disease-free and disease-specific survival (P = .044; P = .049), specifically among patients treated with adjuvant chemotherapy. We conclude that ALDH1 associates with deregulated β-catenin signaling, supporting the role of ALDH1 in rectal cancer stemness. ALDH1 expression relates to poor outcome in early stage rectal cancer, a group where new prognostic tools are particularly needed, and may indicate chemo- and radioresistance.
authors: Department of Oncology and Radiotherapy, University of Turku and Turku University Hospital, Hämeentie 11, FIN-20521, Turku, Finland. stavor@utu.fi
authors: S Tuulia
authors: ST
authors: Avoranta
authors: Eija A
authors: EA
authors: Korkeila
authors: Raija H
authors: RH
authors: Ristamäki
authors: Kari J
authors: KJ
authors: Syrjänen
authors: Olli M
authors: OM
authors: Carpén
authors: Seppo O
authors: SO
authors: Pyrhönen
authors: Jari T T
authors: JT
authors: Sundström
mesh_terms: D000368:Aged; D000080924:Aldehyde Dehydrogenase 1 Family; D014408:Biomarkers, Tumor / Q000032:analysis*; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D059248:Chemoradiotherapy; D013505:Digestive System Surgical Procedures; D018572:Disease-Free Survival; D019008:Drug Resistance, Neoplasm; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis / Q000096:biosynthesis*; D053208:Kaplan-Meier Estimate; D008297:Male; D009367:Neoplasm Staging; D016016:Proportional Hazards Models; D012004:Rectal Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D050697:Retinal Dehydrogenase / Q000032:analysis / Q000096:biosynthesis*; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor; D007527:Isoenzymes; D000080924:Aldehyde Dehydrogenase 1 Family; C510223:ALDH1A1 protein, human; D050697:Retinal Dehydrogenase