title: Thrombin and plasmin-like activities in the latices of Cryptostegia grandiflora and Plumeria rubra.
abstract: Latex proteins have drawn attention because they have shown several pharmacological activities. Herein, the fibrin(ogen)olytic activity of Cryptostegia grandiflora (CgLP) and Plumeria rubra (PrLP) latices were evaluated and characterized. Ion-exchange chromatography separated CgLP in proteolytic (CgLP PI) and nonproteolytic proteins (CgLP PII). CgLP and CgLP PI hydrolyzed azocasein in a dose-dependent manner, whereas CgLP PII and PrLP showed negligible activities. CgLP and CgLP PI accelerated plasmatic clot formation and digested all fibrinogen chains in a time/dose-dependent manner, though in a nonspecific way. CgLP and CgLP PI did not fully hydrolyze the subunits of the fibrin clot since fibrin α-chain showed resistance to proteolysis. No fibrinogenolytic activity was noticed after incubation of CgLP and CgLP PI with E-64. These results suggested that fibrinogenolytic and procoagulant activities of C. grandiflora were performed by cysteine proteases and confirm the activity of latex cysteine proteases as thrombin and plasmin-like proteins.
authors: Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil.
authors: Carolina A
authors: CA
authors: Viana
authors: Jefferson S
authors: JS
authors: Oliveira
authors: Cleverson D T
authors: CD
authors: Freitas
authors: Nylane M N
authors: NM
authors: Alencar
authors: Cristina P S
authors: CP
authors: Carvalho
authors: Beatriz C
authors: BC
authors: Nishi
authors: Márcio V
authors: MV
authors: Ramos
mesh_terms: D029062:Apocynaceae / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005340:Fibrinogen / Q000737:chemistry*; D005341:Fibrinolysin / Q000737:chemistry / Q000302:isolation & purification; D005342:Fibrinolysis; D006801:Humans; D007840:Latex / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D010949:Plasma; D058105:Polymerization; D059748:Proteolysis; D013917:Thrombin / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D007840:Latex; D010940:Plant Proteins; D005340:Fibrinogen; D013917:Thrombin; D005341:Fibrinolysin