title: Scotty: a web tool for designing RNA-Seq experiments to measure differential gene expression.
abstract: BACKGROUND
  A common question arises at the beginning of every experiment where RNA-Seq is used to detect differential gene expression between two conditions: How many reads should we sequence?
  
  
  RESULTS
  Scotty is an interactive web-based application that assists biologists to design an experiment with an appropriate sample size and read depth to satisfy the user-defined experimental objectives. This design can be based on data available from either pilot samples or publicly available datasets.
  
  
  BACKGROUND
  Scotty can be freely accessed on the web at http://euler.bc.edu/marthlab/scotty/scotty.php
authors: Department of Biology, Boston College, Chestnut Hill, MA 02467, USA.
authors: Michele A
authors: MA
authors: Busby
authors: Chip
authors: C
authors: Stewart
authors: Chase A
authors: CA
authors: Miller
authors: Krzysztof R
authors: KR
authors: Grzeda
authors: Gabor T
authors: GT
authors: Marth
mesh_terms: D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D020407:Internet; D017423:Sequence Analysis, RNA / Q000379:methods*; D012984:Software