title: Genotyping of calves rotavirus in China by reverse transcription polymerase chain reaction.
abstract: To investigate the epidemical characteristics and genotype distributions of bovine rotavirus (BRV) in China, 195 fecal samples were collected from calves with diarrhea in China. Fecal samples were detected for rotavirus A antigen using ELISA. The positive samples were screened for VP7 and VP4 by RT-PCR. G serotyping and P genotyping were conducted on 53 VP7 and VP4 positive samples using RT-PCR. The results showed that 82 samples were found positive for BRV. 752bp, 660bp and 285bp bands were amplified for G-typing. 478bp, 375bp and 361bp bands were amplified for P-typing. The G6 and G10 serotypes were 29 (54.7%) and 8 (15.1%) in positive samples for VP7. P[5] and P[11] genotypes were 28 (52.8%) and 10 (18.9%) in the positive samples for VP4. The main combinations of BRV G serotype and P genotype were G6P[5] (28.3%), G6P[5]P[11] (5.7%), G10P[5] (5.7%) and G10G6P[5] (5.7%), respectively. Other combinations (including untypable) of G serotype and P genotype were 54.6%. The dominant G serotype and P genotype were G6 and P[5] respectively. The predominant combination of G and P serotypes was G6P[5]. This has significance for establishing the preventive measures against diarrhea caused by group A rotaviruses in cattle.
authors: Life Science and Engineering College, Northwest University for Nationalities, Lanzhou 730030, China. weisc668@163.com
authors: Suocheng
authors: S
authors: Wei
authors: Zhuandi
authors: Z
authors: Gong
authors: Tuanjie
authors: T
authors: Che
authors: Ayimu
authors: A
authors: Guli
authors: Fengling
authors: F
authors: Tian
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology; D036022:Capsid Proteins / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000235:genetics / Q000821:virology*; D002681:China / Q000453:epidemiology; D003967:Diarrhea / Q000662:veterinary / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000821:virology; D005838:Genotype; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012401:Rotavirus / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D012703:Serotyping / Q000662:veterinary
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D012367:RNA, Viral; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus