title: Biological components in a standardized derivative of bovine colostrum.
abstract: Products of different origin, time of collection, and activities fall under the general term of colostrum and, therefore, great variability in composition as well as in the concentration of its components has been reported in the literature. In the present study, we describe the standardization of a bovine colostrum derivative and the characterization of its bioactive components. Evaluation of the most representative agents (lactoferrin, transferrin, IL-2, IFN-γ, tumor necrosis factor, IgG, and IgA) showed that a marked decrease in active components occurs after the first few hours. Bovine colostrum was, therefore, collected up to the fifth hour after delivery from Holstein cows, in the presence of preservatives, and immediately frozen. A protocol of centrifugation, filtration, and lyophilization was then applied to pools of colostrum from at least 30 cows to obtain a stable, sterile, standardized product. Preservatives were removed by dialysis. Evaluation of the active biological components of colostrum showed that the final product of colostrums contained significant and reproducible amounts of bioactive factors, including cytokines, immunomodulating factors, growth factors, and immunoglobulins. The final product appeared, therefore, as a sterile, pyrogen-free, standardized derivative of bovine colostrum with a high concentration of bioactive components.
authors: Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, 20129 Milan, Italy.
authors: P
authors: P
authors: Sacerdote
authors: F
authors: F
authors: Mussano
authors: S
authors: S
authors: Franchi
authors: A E
authors: AE
authors: Panerai
authors: G
authors: G
authors: Bussolati
authors: S
authors: S
authors: Carossa
authors: A
authors: A
authors: Bartorelli
authors: B
authors: B
authors: Bussolati
mesh_terms: D000818:Animals; D058491:Bacterial Load / Q000662:veterinary; D002417:Cattle; D003126:Colostrum / Q000737:chemistry* / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007371:Interferon-gamma / Q000032:analysis; D007376:Interleukin-2 / Q000032:analysis; D007781:Lactoferrin / Q000032:analysis; D013997:Time Factors; D014168:Transferrin / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007376:Interleukin-2; D014168:Transferrin; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D007781:Lactoferrin