title: Effect of trehalose on the biocontrol efficacy of Pichia caribbica against post-harvest grey mould and blue mould decay of apples.
abstract: BACKGROUND
  The influence of adding trehalose to the culture medium on the efficacy of Pichia caribbica (JSU-1) in controlling post-harvest grey mould and blue mould decay of apples and the possible mechanisms involved were investigated.
  
  
  RESULTS
  The antagonistic activity of P. caribbica harvested from nutrient yeast dextrose broth (NYDB) amended with trehalose at 5 mg mL(-1) to post-harvest grey mould and blue mould decay was improved greatly compared with that without trehalose. The intracellular trehalose content of P. caribbica harvested from NYDB amended with trehalose at 5 mg mL(-1) was higher than that of P. caribbica harvested from NYDB. The population of P. caribbica could rapidly colonise and grow in apple wounds. Moreover, the application of P. caribbica cultivated in the culture medium of the trehalose supplement (5 mg mL(-1) ) induced higher polyphenoloxidase (PPO) and peroxidase (POD) activity of apples compared with that cultivated by P. caribbica in the NYDB. SEM results suggested that P. caribbica harvested from NYDB amended with trehalose at 5 mg mL(-1) showed stronger inhibition efficacy to Botrytis cinerea than P. caribbica harvested from NYDB.
  
  
  CONCLUSIONS
  The antagonistic activity of P. caribbica can be enhanced by adding trehalose to the medium.
authors: College of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu, People's Republic of China.
authors: Lina
authors: L
authors: Zhao
authors: Hongyin
authors: H
authors: Zhang
authors: Hetong
authors: H
authors: Lin
authors: Xiaoyun
authors: X
authors: Zhang
authors: Xiaofeng
authors: X
authors: Ren
mesh_terms: D000898:Antibiosis; D020171:Botrytis / Q000502:physiology*; D004156:Catechol Oxidase / Q000235:genetics / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D005519:Food Preservation; D005638:Fruit / Q000201:enzymology / Q000235:genetics / Q000382:microbiology; D027845:Malus / Q000201:enzymology / Q000235:genetics / Q000382:microbiology*; D010572:Pest Control, Biological; D010843:Pichia / Q000502:physiology*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D014199:Trehalose / Q000378:metabolism*
chemical_list: D003470:Culture Media; D010940:Plant Proteins; D014199:Trehalose; D004156:Catechol Oxidase
keywords: Pichia caribbica; antagonistic activity; apples; blue mould decay; grey mould decay; possible mechanisms; trehalose