title: Interaction between p68 RNA helicase and Ca2+-calmodulin promotes cell migration and metastasis.
abstract: p68 RNA helicase is a prototypical RNA helicase. Here we present evidence to show that, by interacting with Ca-calmodulin, p68 has a role in cancer metastasis and cell migration. A peptide fragment that spans the IQ motif of p68 strongly inhibits cancer metastasis in two different animal models. The peptide interrupts p68 and Ca-calmodulin interaction and inhibits cell migration. Our results demonstrate that the p68-Ca-calmodulin interaction is essential for the formation of lamellipodia and filopodia in migrating cells. p68 interacts with microtubules in the presence of Ca-calmodulin. Our experiments show that interaction with microtubules stimulates p68 ATPase activity. Further, microtubule gliding assays demonstrate that p68, in the presence of Ca-calmodulin, can function as a microtubule motor. This motor activity may allow p68 to transport Ca-calmodulin to the leading edge of migrating cells.
authors: Department of Biology, Georgia State University, Atlanta, Georgia 30303, USA.
authors: Haizhen
authors: H
authors: Wang
authors: Xueliang
authors: X
authors: Gao
authors: Jenny J
authors: JJ
authors: Yang
authors: Zhi-Ren
authors: ZR
authors: Liu
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D053487:DEAD-box RNA Helicases / Q000737:chemistry / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D006801:Humans; D047468:Immunoprecipitation; D051379:Mice; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism; D008954:Models, Biological; D020409:Molecular Motor Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D009369:Neoplasms / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D011485:Protein Binding / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D002147:Calmodulin; D020409:Molecular Motor Proteins; D010455:Peptides; D053487:DEAD-box RNA Helicases; D002118:Calcium