title: Evaluation of peripapillary retinal nerve fiber layer thickness in patients with vitamin B12 deficiency using spectral domain optical coherence tomography.
abstract: OBJECTIVE
  To compare peripapillary retinal nerve fiber layer (RNFL) thicknesses measured by Cirrus HD optical coherence tomography (OCT) of patients with vitamin B12 deficiency with healthy controls and to evaluate the correlation between the peripapillary RNFL thickness and plasma vitamin B12 levels.
  
  
  METHODS
  Forty-five patients (19 male and 26 female) with a diagnosis of vitamin B12 deficiency (patient group) and 45 age- and sex- matched healthy subjects (control group) were consecutively enrolled in this study. Average, temporal, nasal, inferior, and superior quadrant peripapillary RNFL thicknesses of each subject were obtained using the Cirrus HD OCT. Disc area (DA) and rim area (RA), central subfield thickness (CST), cube volume (CV), and cube average thickness (CAT) were also measured.
  
  
  RESULTS
  Mean age of each group was 33.1 ± 6.5 years (range: 21-45 years). Mean plasma vitamin B12 level was 114.8 ± 34.0 pg/mL in the patient group and was 405.1 ± 20.0 pg/mL in the control group (p < 0.001). The patient and control groups were similar regarding axial length, plasma folate levels, DA, RA, CST, CV, CAT, and RNFL thicknesses in superior, nasal, and inferior quadrants. However, average RNFL and RNFL in temporal quadrant were significantly thinner in the patient group than in the control group (p = 0.013 and p < 0.001, respectively). In addition, temporal (r = 0.356, p = 0.001) and average (r = 0.212, p = 0.045) peripapillary RNFL thicknesses were correlated with plasma vitamin B12 levels.
  
  
  CONCLUSIONS
  We have shown that, as in other non-glaucomatous optic neuropathies, temporal quadrant RNFL thickness was thinner in patients with vitamin B12 deficiency and it was correlated with plasma vitamin B12 levels. Further studies are warranted to clarify the clinical relevance of these findings and the effects of vitamin B12 replacement therapy.
authors: Department of Ophthalmology, Recep Tayyip Erdoğan University Medical School, Rize, Turkey.
authors: Kemal
authors: K
authors: Türkyılmaz
authors: Veysi
authors: V
authors: Öner
authors: Ayşegül Küçükali
authors: AK
authors: Türkyılmaz
authors: Aynur
authors: A
authors: Kırbaş
authors: Serkan
authors: S
authors: Kırbaş
authors: Berrak
authors: B
authors: Şekeryapan
mesh_terms: D000328:Adult; D005260:Female; D005901:Glaucoma / Q000097:blood / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009412:Nerve Fibers / Q000473:pathology*; D009898:Optic Disk / Q000473:pathology; D009901:Optic Nerve Diseases / Q000097:blood / Q000473:pathology*; D012160:Retina / Q000473:pathology*; D012162:Retinal Degeneration / Q000097:blood / Q000473:pathology*; D041623:Tomography, Optical Coherence / Q000379:methods*; D014805:Vitamin B 12 / Q000097:blood; D014806:Vitamin B 12 Deficiency / Q000097:blood / Q000473:pathology*; D055815:Young Adult
chemical_list: D014805:Vitamin B 12