title: β-Glucanase specific expression in the parotid gland of transgenic mice.
abstract: The feasibility of using the pig parotid secretory protein promoter to drive the β-glucanase transgene expression in mouse parotid glands was examined in this study. The parotid gland-specific vector expressing β-glucanase gene (GLU, from Paenibacillus polymyxa CP7) was constructed. Transgenic mice were produced by the pronuclear microinjection. Both PCR and Southern blot analysis showed that the mice carried the β-glucanase gene and the β-glucanase gene could be stably inherited. Furthermore, RT-PCR and northern blot analysis indicated that it was specifically expressed in the parotid. The β-glucanase activity in the saliva was found to be 0.18 U/mL. After feeding a diet containing 2 % β-glucan, the average daily gain of transgenic was significantly higher than non-transgenic mice. The crude protein and crude fat concentration in faeces of transgenic mice were significantly reduced compared with that of the non-transgenic mice. These results suggest that the successful expression of foreign β-glucanase in the animal parotid would offer a promising biological approach to reduce the anti-nutritional effect of β-glucans in feed.
authors: College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China. guanlizeng@163.com
authors: Li-zheng
authors: LZ
authors: Guan
authors: Yu-ping
authors: YP
authors: Sun
authors: Qian-yun
authors: QY
authors: Xi
authors: Jing-lan
authors: JL
authors: Wang
authors: Jun-yun
authors: JY
authors: Zhou
authors: Gang
authors: G
authors: Shu
authors: Qing-yan
authors: QY
authors: Jiang
authors: Yong-liang
authors: YL
authors: Zhang
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000235:genetics; D005936:Glucans / Q000235:genetics* / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D056507:Paenibacillus; D010306:Parotid Gland / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D005936:Glucans; D012471:Salivary Proteins and Peptides; D006026:Glycoside Hydrolases