title: Cartilage regeneration by selected chondrogenic clonal mesenchymal stem cells in the collagenase-induced monkey osteoarthritis model.
abstract: Osteoarthritis (OA) is the most common form of arthritis, in which cartilage is irreversibly degraded, causing severe pain and disability. Current therapeutic strategies cannot repair damaged cartilage. We evaluated the repair potential of selected chondrogenic clonal MSCs (sC-MSCs) by delivering them into the injured cartilage site in a collagenase-induced OA model in Cynomolgus monkeys. In vitro characterization showed that the isolated monkey sC-MSCs and polyclonal MSCs (P-MSCs) expressed mesenchymal stem cell markers and could differentiate into chondrocytes. The articular cartilage lesions in animals were treated with normal saline (NS), autologous P-MSCs and sC-MSCs, respectively, by direct delivery. The clinical parameters, radiographic images, histological and immunohistochemical examinations at weeks 8, 16 and 24 post-treatment demonstrated that the abrasions of articular cartilage were significantly improved and repaired by MSC-based treatment, particularly in the sC-MSC-treated group, which displayed consistently higher histological scores than those of other groups. In summary, treatment with sC-MSCs can effectively improve the healing of cartilage lesions in the Cynomolgus monkey collagenase-induced OA model. Due to the genetic proximity of monkey and human, the therapeutic strategy presented in this study will have broad applications in clinical practice.
authors: Department of Surgery, CRCHUM, Notre Dame Hospital, University of Montreal, Quebec, Canada; Department of Orthopaedics, Huashan Hospital, Fudan University, Shanghai, People's Republic of China.
authors: Li
authors: L
authors: Jiang
authors: Anlun
authors: A
authors: Ma
authors: Lijun
authors: L
authors: Song
authors: Yanxin
authors: Y
authors: Hu
authors: Hao
authors: H
authors: Dun
authors: Pierre
authors: P
authors: Daloze
authors: Yonglin
authors: Y
authors: Yu
authors: Jianyuan
authors: J
authors: Jiang
authors: Muhammad
authors: M
authors: Zafarullah
authors: Huifang
authors: H
authors: Chen
mesh_terms: D000818:Animals; D002356:Cartilage / Q000473:pathology / Q000503:physiopathology*; D020219:Chondrogenesis; D002999:Clone Cells; D017364:Collagenases; D004195:Disease Models, Animal; D006801:Humans; D007596:Joints / Q000473:pathology; D008252:Macaca fascicularis; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010003:Osteoarthritis / Q000473:pathology / Q000628:therapy*; D012038:Regeneration; D014945:Wound Healing
chemical_list: D017364:Collagenases; D020782:Matrix Metalloproteinases
keywords: cartilage regeneration; collagenase-induced; mesenchymal stem cells; monkey; osteoarthritis; selected chondrogenic clonal