title: Peste des petits ruminants virus exploits cellular autophagy machinery for replication.
abstract: Peste des petits ruminants virus (PPRV) is an important pathogen that seriously influences the productivity of small ruminants worldwide. Although PPRV is known to induce apoptosis in infected cells, the interaction between PPRV and permissive cells requires further elucidation. Here, we provide the first evidence that PPRV infection triggered autophagy in Vero cells based on the appearance of abundant double- and single-membrane vesicles, the accumulation of LC3 fluorescent puncta, the enhancement of LC3-I/-II conversion, and autophagic flux. We further demonstrated that induction of autophagy with rapamycin significantly increased PPRV progeny yield and nucleocapsid (N) protein expression, while inhibition of autophagy with siRNA targeting ATG7 resulted in diametrically opposite results. Our data indicate that PPRV exploits the autophagy machinery to facilitate its own replication in host cells, thus the production efficiency of live attenuated PPRV vaccines may be improved by targeting the autophagic pathway.
authors: Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100029, People's Republic of China.
authors: Yongning
authors: Y
authors: Zhang
authors: Shaoqiang
authors: S
authors: Wu
authors: Jizhou
authors: J
authors: Lv
authors: Chunyan
authors: C
authors: Feng
authors: Junhua
authors: J
authors: Deng
authors: Caixia
authors: C
authors: Wang
authors: Xiangfen
authors: X
authors: Yuan
authors: Tianyi
authors: T
authors: Zhang
authors: Xiangmei
authors: X
authors: Lin
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology / Q000378:metabolism; D001343:Autophagy / Q000235:genetics; D002522:Chlorocebus aethiops; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D019590:Nucleocapsid Proteins / Q000096:biosynthesis / Q000276:immunology; D018107:Peste-des-petits-ruminants virus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D034622:RNA Interference; D034741:RNA, Small Interfering; D020123:Sirolimus / Q000494:pharmacology; D044764:Ubiquitin-Activating Enzymes / Q000235:genetics / Q000378:metabolism; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000956:Antigens, Viral; D008869:Microtubule-Associated Proteins; D019590:Nucleocapsid Proteins; D034741:RNA, Small Interfering; D044764:Ubiquitin-Activating Enzymes; D020123:Sirolimus