title: Effects of cyclin E gene silencing on the proliferation of esophageal             cancer cell lines, EC9706, Eca109 and KYSE30.
abstract: In order to observe the effects of cyclin E gene silencing by small interfering             RNA (siRNA) on the growth, proliferation, invasion and apoptosis of esophageal             cancer cell lines, including EC9706, Eca109 and KYSE30, siRNA vectors targeting             cyclin E gene were constructed and then transfected into the EC9706, Eca109 and             KYSE30 human esophageal cancer cell lines. Cyclin E mRNA and protein expression             were determined by RT-PCR and western blotting. Cell proliferation and clonality             were detected using a CCK-8 test and soft agar colony formation assay. Cell cycle             distribution, apoptosis and invasion of EC9706, Eca109 and KYSE30 cells were evaluated             with flow cytometry and a transwell culture system. After siRNA vectors targeting             the cyclin E gene were transfected into EC9706, Eca109 and KYSE30 cell lines,             compared with blank and negative control groups, the expression of cyclin E mRNA             and protein (P<0.01), colony-forming units and the number of cells penetrating             the transwell membrane (P<0.05) were significantly decreased, the cells in             the S and G2/M phase were reduced, the cells in the G0/G1 phase were increased             and the apoptosis rate was increased (P<0.01) in the experimental groups. Cyclin E             gene silencing effectively inhibits growth, proliferation and invasion of esophageal             cancer cells.
authors: Department of Microbiology and Immunology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, PR China.
authors: Na
authors: N
authors: Wang
authors: Min
authors: M
authors: Li
authors: Wen-Qiao
authors: WQ
authors: Zang
authors: Yun-Yun
authors: YY
authors: Ma
authors: Yuan-Yuan
authors: YY
authors: Wang
authors: Guo-Qiang
authors: GQ
authors: Zhao
mesh_terms: D017209:Apoptosis; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019927:Cyclin E / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism; D006801:Humans; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism
chemical_list: D019927:Cyclin E; D012333:RNA, Messenger; D034741:RNA, Small Interfering