title: [Relationship between heat shock protein 70 expression and radiotherapeutic effect in esophageal squamous cell carcinoma].
abstract: OBJECTIVE
  To explore the relationship between heat shock protein 70 (HSP70) expression and radiotherapeutic sensitivity.
  
  
  METHODS
  Retrospective analysis was conducted for HSP70 expression in surgical specimens of 70 patients with esophageal cancer and their radiotherapy effect; the siRNA plasmids were prepared to inhibit HSP70 expression. After culturing, the plasmids were transfected into EC9706 cells. After γ-ray irradiation, the cell numbers were compared with the control group. And the contents of HSP70 protein were measured by Western blot in transfected cells.
  
  
  RESULTS
  In HSP positive group, patients with chemotherapy or chemoradiotherapy had a lower 2-year recurrence rate than the radiotherapy group (35% vs 55%). In HSP-negative group, there was no significant difference. After γ-ray irradiation, the number of RNA interference cell lines was significantly less than that of the RNA expression cell lines. Western blot showed that, after γ-ray irradiation, HSP70 expression increased, peaked at 16 hours and began to decline at 20 hours.
  
  
  CONCLUSIONS
  Esophageal squamous cell carcinoma is not sensitive to radiotherapy. The interference of HSP70 protein synthesis may become a new method of improving the efficacy of radiotherapy.
authors: Medical Oncology Department, People's Hospital of Zhengzhou, Department of Oncology, Zhengzhou 450000, China.
authors: Xiang-rui
authors: XR
authors: Meng
authors: Peng
authors: P
authors: Lu
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy*; D045744:Cell Line, Tumor; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy*; D000077277:Esophageal Squamous Cell Carcinoma; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D010957:Plasmids; D014162:Transfection
chemical_list: D018840:HSP70 Heat-Shock Proteins