title: Fluoride modulates preosteoblasts viability and matrix metalloproteinases-2 and -9 activities.
abstract: This study evaluated the influence of fluoride on cell viability and activity of matrix metalloproteinases (MMP) -2 and -9 secreted by preosteoblasts. Preosteoblasts (MC3T3-E1 murine cell line) were cultured in MEM medium supplement with 10% Fetal Bovine Serum (FBS) and nucleosides/ribonucleosides without ascorbic acid. Adherent cells were treated with different concentrations of F (as sodium fluoride-NaF) in medium (5 x 10(-6) M, 10(-5) M, 10(-4) M and 10(-3) M) for 24, 48, 72 and 96 h at 37ºC, 5% CO(2). Control cells were cultivated in MEM only. After each period, preosteoblast viability was assessed by MTT assay. MMP-2 and -9 activities were performed by gel zymography. Also, alkaline phosphatase (ALP) activity was quantified by colorimetry in all experimental groups. It was shown that cultured cells with the highest dose of F (10(-3) M) for 96 h decreased preosteoblast viability while lower doses of F did not alter it, when compared to untreated cells. No differences were observed in ALP activity among groups. Moreover, compared to control, the treatment of cells with F at low dose slightly increased MMP-2 and -9 activities after 24 h. It was concluded that F modulates preosteoblast viability in a dose-dependent manner and also may regulate extracellular matrix remodeling.
authors: Department of Biological Sciences, Bauru Dental School, USP - University of São Paulo, Bauru, SP, Brazil.
authors: Camila
authors: C
authors: Slompo
authors: Camila P
authors: CP
authors: Buzalaf
authors: Carla A
authors: CA
authors: Damante
authors: Gisele M
authors: GM
authors: Martins
authors: Angélica R
authors: AR
authors: Hannas
authors: Marília A R
authors: MA
authors: Buzalaf
authors: Rodrigo C
authors: RC
authors: Oliveira
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002245:Carbon Dioxide / Q000008:administration & dosage; D002327:Cariostatic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003124:Colorimetry; D004396:Coloring Agents; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D005109:Extracellular Matrix / Q000187:drug effects; D020778:Matrix Metalloproteinase 2 / Q000187:drug effects*; D020780:Matrix Metalloproteinase 9 / Q000187:drug effects*; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects*; D012969:Sodium Fluoride / Q000008:administration & dosage / Q000494:pharmacology*; D013696:Temperature; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors
chemical_list: D002327:Cariostatic Agents; D004396:Coloring Agents; D003470:Culture Media; D013778:Tetrazolium Salts; D013844:Thiazoles; D002245:Carbon Dioxide; D012969:Sodium Fluoride; D000469:Alkaline Phosphatase; D020778:Matrix Metalloproteinase 2; C522362:Mmp2 protein, mouse; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse; C022616:thiazolyl blue