title: In vitro induction of secondary xylem-like tracheary elements in calli of hybrid poplar (Populus sieboldii × P. grandidentata).
abstract: The formation of tracheary elements was induced in calli derived from petioles of hybrid poplar (Populus sieboldii × P. grandidentata) after 10 days of culture on medium that lacked auxin but contained 1 μM brassinolide. Some differentiated cells formed broad regions of cell walls and bordered pits, which are typical features of tracheary elements of secondary xylem. Other differentiated cells resembled tracheary elements of primary xylem, with spiral or reticulate thickening of cell walls. The tracheary elements that developed in calli were formed within cell clusters. This induction system provides a new model for studies of the mechanism of differentiation of secondary xylem cells in vitro.
authors: Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-Tokyo, 183-8509, Japan.
authors: Yusuke
authors: Y
authors: Yamagishi
authors: Joto
authors: J
authors: Yoshimoto
authors: Hiromu
authors: H
authors: Uchiyama
authors: Eri
authors: E
authors: Nabeshima
authors: Satoshi
authors: S
authors: Nakaba
authors: Ugai
authors: U
authors: Watanabe
authors: Ryo
authors: R
authors: Funada
mesh_terms: D046508:Culture Techniques; D018613:Microscopy, Confocal; D058526:Plant Vascular Bundle / Q000166:cytology*; D032107:Populus / Q000166:cytology* / Q000502:physiology