title: In vitro antiseptic susceptibilities for Staphylococcus pseudintermedius isolated from canine superficial pyoderma in Japan.
abstract: BACKGROUND
  Topical therapy, particularly with chlorhexidine, is becoming increasingly common as a treatment option for canine pyoderma; however, there are limited studies on the susceptibility of Staphylococcus pseudintermedius to chlorhexidine compounds.
  
  
  OBJECTIVE
  To determine the in vitro susceptibility of both meticillin-resistant and meticillin-susceptible S. pseudintermedius isolates to chlorhexidine and other antiseptic agents and the presence of multidrug efflux pump genes.
  
  
  METHODS
  One hundred S. pseudintermedius isolates from 23 initial and 77 recurrent cases of canine pyoderma.
  
  
  METHODS
  After bacterial identification and mecA testing, minimal inhibitory concentrations (MICs) of antiseptic agents were determined. Multidrug efflux pump genes, including qacA, qacB and smr, were identified.
  
  
  RESULTS
  Of the 100 isolates, 57 were identified as meticillin-resistant S. pseudintermedius. The MIC(90) of chlorhexidine acetate, chlorhexidine gluconate, acriflavine, ethidium bromide and benzalkonium chloride were 1, 1, 2, 0.5 and 2 μg/mL, respectively. Multidrug efflux pump genes qacA, qacB and smr were not detected in any of the isolates.
  
  
  CONCLUSIONS
  The MICs for chlorhexidine and other antiseptics remain low, and multidrug efflux pump genes were not found in the tested isolates.
authors: ASC Dermatology Service, 1-3-2 Jindaijihigashi Chofu, Tokyo 182-0012, Japan. muragon@d1.dion.ne.jp
authors: Nobuo
authors: N
authors: Murayama
authors: Masahiko
authors: M
authors: Nagata
authors: Yuri
authors: Y
authors: Terada
authors: Mio
authors: M
authors: Okuaki
authors: Noriyuki
authors: N
authors: Takemura
authors: Hidemasa
authors: H
authors: Nakaminami
authors: Norihisa
authors: N
authors: Noguchi
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004285:Dogs; D015964:Gene Expression Regulation, Bacterial; D007564:Japan / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D011711:Pyoderma / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D013207:Staphylococcal Skin Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D013210:Staphylococcus / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins