title: Gadolinium(3+)-doped mesoporous silica nanoparticles as a potential magnetic resonance tracer for monitoring the migration of stem cells in vivo.
abstract: We investigated the tracking potential of a magnetic resonance imaging (MRI) probe made of gadolinium-doped mesoporous silica MCM-41 (Gd(2)O(3)@MCM-41) nanoparticles for transplanted bone mesenchymal stem cells (MSCs) and neural stem cells (NSCs) in vivo. The nanoparticles, synthesized using a one-step synthetic method, possess hexagonal mesoporous structures with appropriate assembly of nanoscale Gd(2)O(3) clusters. They show little cytotoxicity against proliferation and have a lower effect on the inherent differentiation potential of these labeled stem cells. The tracking of labeled NSCs in murine brains was dynamically determined with a clinical 3T MRI system for at least 14 days. The migration of labeled NSCs identified by MRI corresponded to the results of immunofluorescence imaging. Our study confirms that Gd(2)O(3)@MCM-41 particles can serve as an ideal vector for long-term MRI tracking of MSCs and NSCs in vivo.
authors: Imaging Diagnostic and Interventional Center, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong, China.
authors: Yingying
authors: Y
authors: Shen
authors: Yuanzhi
authors: Y
authors: Shao
authors: Haoqiang
authors: H
authors: He
authors: Yunpu
authors: Y
authors: Tan
authors: Xiumei
authors: X
authors: Tian
authors: Fukang
authors: F
authors: Xie
authors: Li
authors: L
authors: Li
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D058948:Cell Tracking / Q000379:methods*; D005682:Gadolinium / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D008279:Magnetic Resonance Imaging / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D009132:Muscles / Q000166:cytology; D053758:Nanoparticles / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012822:Silicon Dioxide / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: C030581:gadolinium oxide; D012822:Silicon Dioxide; D005682:Gadolinium
keywords: gadolinium; magnetic resonance imaging; nanoparticles; stem cells