title: Overexpression of GPC3 inhibits hepatocellular carcinoma cell proliferation             and invasion through induction of apoptosis.
abstract: Glypican‑3 (GPC3) is a membrane heparan sulfate proteoglycan involved in             cell proliferation, differentiation, adhesion, migration and the development of             the majority of mesodermal tissues and organs. GPC3 has been found to be important             for the occurrence and development of hepatocellular carcinoma (HCC). Therefore,             it may be suitable for use as a novel molecular marker for the diagnosis of primary             liver cancer. In the present study, the role of GPC3 in the occurrence and development             of HCC was determined. GPC3 recombinant vector was transfected into two HCC cell             lines, Huh7 and SK‑HEP‑1, to upregulate the expression of GPC3 and examine changes             in the biological behavior of the cells. Results indicate that overexpression             of GPC3 in Huh7 and SK‑HEP‑1 cells effectively inhibited cell proliferation and             cell invasion through induction of apoptosis. However, cotreatment of the cells             with insulin‑like growth factor 2 (IGF2) and fibroblast growth factor 2 (FGF2)             was found by Annexin V‑PI flow cytometric analysis to significantly inhibit the             apoptotic cell death induced by GPC3 overexpression. These observations indicate             that GPC3 may act as a negative regulator of IGF2 and FGF2 pathways. Taken together,             these results demonstrate that overexpression of GPC3 inhibits the occurrence             and development of HCC.
authors: Second Department of General Surgery, Fifth Hospital of Wuhan, Hubei 430050, PR China. panzhijian1969@sina.com
authors: Zhijian
authors: Z
authors: Pan
authors: Chunzhou
authors: C
authors: Chen
authors: Haocheng
authors: H
authors: Long
authors: Changjiang
authors: C
authors: Lei
authors: Gang
authors: G
authors: Tang
authors: Lei
authors: L
authors: Li
authors: Jiarui
authors: J
authors: Feng
authors: Feixiang
authors: F
authors: Chen
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005822:Genetic Vectors / Q000378:metabolism; D053673:Glypicans / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D014162:Transfection
chemical_list: D053673:Glypicans; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D007335:Insulin-Like Growth Factor II