title: Reevaluation of the requirement for TIP47 in human immunodeficiency virus type 1 envelope glycoprotein incorporation.
abstract: Incorporation of the human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins into assembling particles is crucial for virion infectivity. Genetic and biochemical data indicate that the matrix (MA) domain of Gag and the cytoplasmic tail of the transmembrane glycoprotein gp41 play an important role in coordinating Env incorporation; however, the molecular mechanism and possible role of host factors in this process remain to be defined. Recent studies suggested that Env incorporation is mediated by interactions between matrix and tail-interacting protein of 47 kDa (TIP47; also known as perilipin-3 and mannose-6-phosphate receptor-binding protein 1), a member of the perilipin, adipophilin, TIP47 (PAT) family of proteins implicated in protein sorting and lipid droplet biogenesis. We have confirmed by nuclear magnetic resonance spectroscopy titration experiments and surface plasmon resonance that MA binds TIP47. We also reevaluated the role of TIP47 in HIV-1 Env incorporation in HeLa cells and in the Jurkat T-cell line. In HeLa cells, TIP47 overexpression or RNA interference (RNAi)-mediated depletion had no significant effect on HIV-1 Env incorporation, virus release, or particle infectivity. Similarly, depletion of TIP47 in Jurkat cells did not impair HIV-1 Env incorporation, virus release, infectivity, or replication. Our results thus do not support a role for TIP47 in HIV-1 Env incorporation or virion infectivity.
authors: Center for Cancer Research, National Cancer Institute, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
authors: Mary Ann
authors: MA
authors: Checkley
authors: Benjamin G
authors: BG
authors: Luttge
authors: Peter Y
authors: PY
authors: Mercredi
authors: Sampson K
authors: SK
authors: Kyere
authors: Justin
authors: J
authors: Donlan
authors: Tsutomu
authors: T
authors: Murakami
authors: Michael F
authors: MF
authors: Summers
authors: Simon
authors: S
authors: Cocklin
authors: Eric O
authors: EO
authors: Freed
mesh_terms: D015497:HIV-1 / Q000502:physiology*; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D019169:Jurkat Cells; D009682:Magnetic Resonance Spectroscopy; D000070796:Perilipin-3; D020349:Surface Plasmon Resonance; D033921:Vesicular Transport Proteins / Q000378:metabolism*; D019065:Virus Assembly; D054299:env Gene Products, Human Immunodeficiency Virus / Q000378:metabolism*
chemical_list: C038196:PLIN3 protein, human; D000070796:Perilipin-3; D033921:Vesicular Transport Proteins; D054299:env Gene Products, Human Immunodeficiency Virus