title: Electron microscope detection of an endogenous infection of retrovirus-like particles in L20B cells.
abstract: L20B cells are a cell line commonly used for the isolation of poliovirus. The current study indicates that L20B cells are chronically infected with a retrovirus-like particle that replicates in the cytoplasm and buds through the plasma membrane. The findings indicate that care is needed in the use of L20B cells for certain virus isolation studies and emphasize the importance of electron microscope studies as an adjunct to the development of diagnostic virology protocols.
authors: Victorian Infectious Diseases Reference Laboratory, 10 Wreckyn Street, North Melbourne, Victoria 3051, Australia.
authors: Jason A
authors: JA
authors: Roberts
authors: Bruce R
authors: BR
authors: Thorley
authors: Leesa D
authors: LD
authors: Bruggink
authors: John A
authors: JA
authors: Marshall
mesh_terms: D000818:Animals; D002460:Cell Line; D051379:Mice; D008854:Microscopy, Electron / Q000379:methods*; D018626:Retroelements; D012190:Retroviridae / Q000302:isolation & purification*; D012192:Retroviridae Infections; D057074:Virus Release
chemical_list: D018626:Retroelements
keywords: L20B cells; poliovirus; retrovirus-like particles