title: Gadolinium- and manganite-based contrast agents with fluorescent probes for both magnetic resonance and fluorescence imaging of pancreatic islets: a comparative study.
abstract: Three magnetic resonance (MR)/fluorescence imaging probes were tested for visualization, cellular distribution, and survival of labeled pancreatic islets in vitro and following transplantation. As T(1) contrast agents (CAs), gadolinium(III) complexes linked to β-cyclodextrin (Gd-F-βCD) or bound to titanium dioxide (TiO2 @RhdGd) were tested. As a T(2) CA, perovskite manganite nanoparticles (LSMO@siF@si) were examined. Fluorescein or rhodamine was incorporated as a fluorescent marker in all probes. Islets labeled with gadolinium(III) CAs were visible as hyperintense spots on MR in vitro, but detection in vivo was inconclusive. Islets labeled with LSMO@siF@si CA were clearly visible as hypointense spots or areas on MR scans in vitro as well as in vivo. All CAs were detected inside the islet cells by fluorescence. Although the vitality and function of the labeled islets was not impaired by any of the tested CAs, results indicate that LSMO@siF@si CA is a superior marker for islet labeling, as it provides better contrast enhancement within a shorter scan time.
authors: Laboratory of Pancreatic Islets, Institute for Clinical and Experimental Medicine (IKEM), Videnska 1958/9, 14021 Prague, Czech Republic.
authors: Zuzana
authors: Z
authors: Berkova
authors: Daniel
authors: D
authors: Jirak
authors: Klara
authors: K
authors: Zacharovova
authors: Ivan
authors: I
authors: Lukes
authors: Zuzana
authors: Z
authors: Kotkova
authors: Jan
authors: J
authors: Kotek
authors: Michal
authors: M
authors: Kacenka
authors: Ondrej
authors: O
authors: Kaman
authors: Ivan
authors: I
authors: Rehor
authors: Milan
authors: M
authors: Hajek
authors: Frantisek
authors: F
authors: Saudek
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003287:Contrast Media / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005682:Gadolinium / Q000737:chemistry*; D007515:Islets of Langerhans / Q000166:cytology / Q000000981:diagnostic imaging / Q000378:metabolism*; D008279:Magnetic Resonance Imaging; D008297:Male; D017895:Manganese Compounds / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry; D008856:Microscopy, Fluorescence; D011859:Radiography; D051381:Rats; D011917:Rats, Inbred Lew; D014025:Titanium / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D003287:Contrast Media; D005456:Fluorescent Dyes; D017895:Manganese Compounds; D047392:beta-Cyclodextrins; C494384:manganite; C009495:titanium dioxide; D005682:Gadolinium; D014025:Titanium; C031215:betadex