title: Insights into TIM-barrel prenyl transferase mechanisms: crystal structures of PcrB from Bacillus subtilis and Staphylococcus aureus.
abstract: Well structured: As a new triose phosphate isomerase (TIM) barrel-fold prenyl transferase, PcrB catalyzes the production of heptaprenylglyceryl phosphate from heptaprenyl diphosphate and glycerol-1-phosphate. Crystal structures of PcrB from Bacillus subtilis and Staphylococcus aureus in complex with ligands were solved, and together with site-directed mutagenesis and bioinformatics analyses, clearly reveal the catalytic mechanism of the enzyme.
authors: Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 XiQiDao, Tianjin 300308, China.
authors: Feifei
authors: F
authors: Ren
authors: Xinxin
authors: X
authors: Feng
authors: Tzu-Ping
authors: TP
authors: Ko
authors: Chun-Hsiang
authors: CH
authors: Huang
authors: Yumei
authors: Y
authors: Hu
authors: Hsiu-Chien
authors: HC
authors: Chan
authors: Yi-Liang
authors: YL
authors: Liu
authors: Ke
authors: K
authors: Wang
authors: Chun-Chi
authors: CC
authors: Chen
authors: Xuefei
authors: X
authors: Pang
authors: Miao
authors: M
authors: He
authors: Yujie
authors: Y
authors: Li
authors: Eric
authors: E
authors: Oldfield
authors: Rey-Ting
authors: RT
authors: Guo
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001412:Bacillus subtilis / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D013211:Staphylococcus aureus / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D014305:Triose-Phosphate Isomerase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D019883:Alkyl and Aryl Transferases; D014305:Triose-Phosphate Isomerase