title: Anti-hepatitis B virus effects of dehydrocheilanthifoline from Corydalis saxicola.
abstract: In this report, the anti-hepatitis B virus (HBV) activity of dehydrocheilanthifoline (DHCH), a quaternary ammonium alkaloid isolated from the traditional Chinese medicine Corydalis saxicola Bunting (Papaveraceae), was determined in vitro. Following six days of treatment, DHCH efficiently suppressed the secretions of HBsAg and HBeAg in HepG2.2.15 cell cultures, with a half-maximal inhibitory concentration (IC(50)) of 15.84 and 17.12 μM, and with a therapeutic index (TI) of 7.32 and 6.77, respectively. Further studies revealed that DHCH reduced the levels of extracellular DNA, intracellular DNA and covalently closed circular DNA (cccDNA) of HBV in a dose-dependent and time-dependent manner, with IC(50) values of 15.08, 7.62 and 8.25 μM, respectively after six days of treatment. In contrast, the level of viral pre-genomic RNA (pgRNA) increased 6.13-fold after treatment with DHCH. Together, it was demonstrated for the first time that DHCH could significantly inhibit the replication of HBV, which warrants further studies on the antiviral mechanisms of DHCH, and suggests that it may be a promising candidate in the therapy of HBV infection.
authors: Jinan Biomedicine Research and Development Center, Jinan University, Guangzhou 510632, P. R. China.
authors: Fan-Li
authors: FL
authors: Zeng
authors: Yang-Fei
authors: YF
authors: Xiang
authors: Zhen-Ran
authors: ZR
authors: Liang
authors: Xiao
authors: X
authors: Wang
authors: Dan-E
authors: DE
authors: Huang
authors: Sui-Nan
authors: SN
authors: Zhu
authors: Min-Min
authors: MM
authors: Li
authors: De-Po
authors: DP
authors: Yang
authors: Dong-Mei
authors: DM
authors: Wang
authors: Yi-Fei
authors: YF
authors: Wang
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D031311:Corydalis; D004279:DNA, Viral / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006514:Hepatitis B Surface Antigens / Q000378:metabolism; D006513:Hepatitis B e Antigens / Q000378:metabolism; D006515:Hepatitis B virus / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D022781:Hepatocytes / Q000187:drug effects / Q000821:virology*; D006801:Humans; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D013997:Time Factors; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000470:Alkaloids; D000998:Antiviral Agents; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D000644:Quaternary Ammonium Compounds; C581819:dehydrocheilanthifoline