title: [Doxorubicin preconditioning instead of ischemic preconditioning in providing ischemic tolerance for rats abdomen island flaps].
abstract: OBJECTIVE
  To investigate the effects and mechanism of doxorubicin preconditioning in providing ischemic tolerance for rats abdomen island flaps.
  
  
  METHODS
  Twenty-four healthy adult Sprague Dawley rats, 12 males and 12 females, were randomly divided into 3 groups (n = 8): control group (group A), ischemic preconditioning group (group B), and doxorubicin preconditioning group (group C). After the abdomen island flap (6 cm x 3 cm in size) based on the superficial inferior epigastric neurovascular bundle was prepared, group A had no further treatment; group B was given a 10-minute ischemia followed by a 10-minute reperfusion for 4 times; and group C was given pretreatment with doxorubicin (1 mg/kg) by injection of the inferior epigastric vein. After 24 hours, the inferior epigastric vessels were blocked by vascular clamp for 4 hours, followed by reperfusion 2 hours to prepare ischemia/reperfusion (I/R) injury model. The rat survival was observed after operation; at 0, 8, 12, 24, and 30 hours after I/R injury, the malonyldiadehyde (MDA) and superoxide dismutase (SOD) levels were measured. At 7 days after I/R injury, the survival rate of flap were calculated and the flaps were harvested for histological observation.
  
  
  RESULTS
  During experiment, 5 rats died (1 rat in groups A and B respectively, 3 rats in group C) and were added. The survival rates of the flap in group A (10.10% +/- 0.43%) was lower than those in group B (91.63% +/- 1.76%) and in group C (92.75% +/- 1.48%) at 7 days after I/R injury, showing significant differences (P < 0.05), and there was no significant difference between groups B and C (t = 0.29, P = 0.77). Significant difference was found in MDA level and SOD level between group A and groups B, C after 8 hours (P < 0.05), and there was no significant difference between groups B and C (P > 0.05). Histological observation showed that inflammatory cells infiltration was more obvious and hyperplasia of fibers was weaker in group A than in groups B and C.
  
  
  CONCLUSIONS
  Doxorubicin preconditioning can provide ischemic tolerance for rats abdomen island flaps and protect flaps from the I/R injury. The possible mechanism may be related to that doxorubicin can induce endogenous protections.
authors: Department of Burns and Plastic Surgery, the Second People's Hospital of Chengdu, Chengdu Sichuan, 610017, P.R.China.
authors: Hong
authors: H
authors: Li
authors: Yin
authors: Y
authors: Cen
authors: Zhenyu
authors: Z
authors: Zhang
mesh_terms: D000005:Abdomen; D000818:Animals; D004317:Doxorubicin / Q000494:pharmacology*; D005240:Feasibility Studies; D005260:Female; D006085:Graft Survival; D019194:Ischemic Preconditioning / Q000379:methods*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D009336:Necrosis; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000032:analysis; D013524:Surgical Flaps / Q000098:blood supply* / Q000473:pathology; D013997:Time Factors
chemical_list: D008315:Malondialdehyde; D004317:Doxorubicin; D013482:Superoxide Dismutase