title: Analysis of differentially expressed genes and microRNAs in alcoholic             liver disease.
abstract: The purpose of this study was to screen differentially expressed genes and             microRNAs in order to find a new target for the accurate diagnosis and effective             therapy of alcoholic liver disease (ALD) at the gene and microRNA levels. The             total RNA of liver tissues was extracted from four groups of patients, ten subjects             each. Microarrays were utilized to detect differentially expressed genes and microRNAs.             According to gene values, significance levels and false discovery rate with a             random variance model, gene ontology (GO) and the Kyoto Encyclopedia of Genes             and Genomes (KEGG) database, node genes and key microRNAs in networks were obtained             and analyzed. A total of 878 differentially expressed genes and 26 microRNAs were             found. In co-expression genetic networks, node genes modulating the network were             Acyl-coenzyme A synthetase-3 (ACSF3), Frizzled-5 (FZD5), LOC727987 and C1orf222.             In microRNA-gene networks, the key microRNAs were hsa-miR-570, hsa-miR-122, hsa-miR-34b,             hsa-miR-29c, hsa-miR-922 and hsa-miR-185, which negatively regulated approximately             79 downstream target genes. In the course of ALD, we found 4 differentially expressed             node genes and analyzed ACSF3 and FZD5. ACSF3 was significantly upregulated, and             was involved in fatty acid and lipid metabolism and accelerated liver injury.             These two genes were involved in fatty acids and lipid metabolism. FZD5 was downregulated             and reduced the synthesis of membrane transport protein in the hepatic membrane             and the membrane stability, and accelerated the liver cell apoptosis process.             Six key microRNAs regulated numerous biological functions such as the immune response,             the inflammatory response and glutathione metabolism. This finding provides valuable             insight into the diagnosis and treatment of ALD.
authors: Department of Gastroenterology, Heilongjiang Province Hospital, Harbin, Heilongjiang 150036, PR China.
authors: Ying
authors: Y
authors: Liu
authors: Shao-Hua
authors: SH
authors: Chen
authors: Xi
authors: X
authors: Jin
authors: You-Ming
authors: YM
authors: Li
mesh_terms: D000328:Adult; D000368:Aged; D003066:Coenzyme A Ligases / Q000235:genetics* / Q000378:metabolism; D051157:Frizzled Receptors / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D006505:Hepatitis; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D008103:Liver Cirrhosis / Q000235:genetics; D008108:Liver Diseases, Alcoholic / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: C498589:FZD5 protein, human; D051157:Frizzled Receptors; D035683:MicroRNAs; C561770:ACSF3 protein, human; D003066:Coenzyme A Ligases