title: Suppression of cell proliferation and collagen production in cultured             human hypertrophic scar fibroblasts by Sp1 decoy oligodeoxynucleotide.
abstract: Hypertrophic scars are characterized by the abnormal proliferation of fibroblasts             and an overproduction of collagen. The Sp1 transcription factor is involved in             the stimulation of collagen synthesis. A decoy oligonucleotide (ODN) targeting             Sp1 was designed and transfected into hypertrophic scar fibroblasts (HSFs) cells             using cationic liposomes. The transfection efficiency was determined by flow cytometry             and was observed to be 85±7% (n=5). Specific binding of the Sp1 decoy ODN was             monitored with an electrophoretic mobility shift assay (EMSA). Following transfection             with the decoy ODN to Sp1, cell viability and cell proliferation, which were examined             by the cell counting kit WST‑8, were decreased by 80% compared with untreated             cells. Transforming growth factor‑β (TGF‑β) mRNA and collagen mRNA expression             were also reduced by 48% in the transfection decoy ODN group. The cell viability             of HSFs after 48 h of transfection with 25, 50, 100 and 150 nM Sp1 decoy ODN was             0.9331±0.0203, 0.7479±0.0868, 0.577±0.0347 and 0.4703±0.0147, respectively. The             100 nM dose of the Sp1 decoy ODN inhibited the expression of types I and III collagen             by 32 and 28%, respectively (both P<0.01). TGF‑β mRNA expression was also effectively             suppressed by the 100 nM Sp1 decoy ODN (P<0.01). The Sp1 decoy ODN inhibited             cell proliferation and the expression of types I and III collagen. Therefore,             Sp1 decoy ODNs may be a promising tool for developing and testing novel therapeutic             applications for treating hypertrophic scars.
authors: Department of Plastic Surgery, Shanghai 6th People's Hospital, Shanghai Jiaotong University, Shanghai 200233, PR China.
authors: Chenliang
authors: C
authors: Deng
authors: Jianghong
authors: J
authors: Zheng
authors: Weidong
authors: W
authors: Wan
authors: Shixin
authors: S
authors: Zhang
authors: Zhi
authors: Z
authors: Ding
authors: Guangyu
authors: G
authors: Mao
authors: Songlin
authors: S
authors: Yang
mesh_terms: D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000378:metabolism*; D024042:Collagen Type I / Q000235:genetics* / Q000378:metabolism; D024061:Collagen Type III / Q000235:genetics* / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008297:Male; D016376:Oligonucleotides, Antisense / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D016329:Sp1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D016329:Sp1 Transcription Factor; D016212:Transforming Growth Factor beta