title: Effect of chronic renal failure medium on the ubiquitin‑proteasome pathway             of arterial muscle cells.
abstract: The ubiquitin‑proteasome pathway (UPP) is involved in the occurrence and             development of atherosclerosis through inhibitor of κB (IκB) degradation which             activates nuclear factor-κB (NF‑κB). However, the correlation between UPP and             vascular complications of uramia remains unknown. The aim of the present study             was to determine whether the UPP is activated in aortic smooth muscle cells (ASMCs)             when cultured with uremic serum and to examine the role of the UPP on the dysfunction             of ASMCs in uremia. ASMCs were cultured with pooled normal sera or chronic renal             failure sera. The mRNA expression levels for ubiquitin (Ub) and Ub-activating             enzyme (E1) were analyzed using reverse transcription PCR and levels of the ubiquitinated             proteins E1 and IκBα were measured using western blot analysis. The enzymatic             activities of three 20S proteasomes were examined using specific fluorogenic peptide             substrates. Compared with normal serum, chronic renal serum increased E1 mRNA             and protein expression of rabbit ASMCs (both P<0.01). In addition, the mRNA             expression of Ub also increased and the expression of IκBα was observed to decrease             significantly (both P<0.01). Ubiquitinated proteins in the normal and chronic             renal failure groups were not found to be significantly different, but the activity             of proteasomes increased significantly (P<0.01). Chronic renal failure medium             induced the activation of the UPP in ASMCs.
authors: Department of Nephrology, the Affiliated Jiangyin Hospital of Southeast University Medical College, Jiangyin 214400, PR China. yaoquanzhang@yeah.net
authors: Yao-Quan
authors: YQ
authors: Zhang
authors: Bing
authors: B
authors: Feng
authors: Fa-Huan
authors: FH
authors: Yuan
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D020672:I-kappa B Proteins / Q000378:metabolism; D007676:Kidney Failure, Chronic / Q000378:metabolism / Q000473:pathology; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000072000:NF-KappaB Inhibitor alpha; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D015398:Signal Transduction; D025801:Ubiquitin / Q000235:genetics / Q000378:metabolism*; D044764:Ubiquitin-Activating Enzymes / Q000235:genetics / Q000378:metabolism; D054875:Ubiquitination
chemical_list: D017077:Culture Media, Conditioned; D020672:I-kappa B Proteins; D012333:RNA, Messenger; D025801:Ubiquitin; D000072000:NF-KappaB Inhibitor alpha; D046988:Proteasome Endopeptidase Complex; D044764:Ubiquitin-Activating Enzymes