title: Development of a robust, low cost stem-loop real-time quantification PCR technique for miRNA expression analysis.
abstract: Development of a rapid and accurate quantification method for the detection of microRNAs (miRNAs) has been desired, in particular, when they are differently expressed in normal and pathological conditions. However, various methods for the quantification of small non-coding RNAs as well as miRNAs have been described. These methods mainly include hybridization-based approaches such as primer extension, northern blotting, microarray profiling, and reverse transcription (RT) PCR. Here, we developed a simple and rapid method based on stem-loop primer-based real-time PCR assay for sensitive and accurate detection of mature miRNAs. Initially, a miRNA-specific stem-loop RT primer is used for RT, which is followed by TaqMan real-time PCR assay using specific forward primer in combination with universal reverse primer and TaqMan probe. The assay has shown high sensitivity (≤50 copies/reaction) for miRNA detection in two breast cancer cell lines, MCF-7 and MDA-MB-231. This assay might be implicated as a rapid and cost effective method for the detection of small non-coding RNAs.
authors: Molecular Medicine Department, Biotechnology Research Center, Pasteur Institute of Iran, P.O. Box 1316943551, Tehran, Iran.
authors: Samira
authors: S
authors: Mohammadi-Yeganeh
authors: Mahdi
authors: M
authors: Paryan
authors: Siamak
authors: S
authors: Mirab Samiee
authors: Masoud
authors: M
authors: Soleimani
authors: Ehsan
authors: E
authors: Arefian
authors: Keyhan
authors: K
authors: Azadmanesh
authors: Ehsan
authors: E
authors: Mostafavi
authors: Reza
authors: R
authors: Mahdian
authors: Morteza
authors: M
authors: Karimipoor
mesh_terms: D002460:Cell Line; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D055029:Inverted Repeat Sequences; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger