title: Molecular variability among isolates of Fusarium oxysporum associated with root rot disease of Agave tequilana.
abstract: In this study, 115 isolates of Fusarium oxysporum from roots of Agave tequilana Weber cv azul plants and soil in commercial plantations in western Mexico were characterized using morphological and molecular methods. Genetic analyses of monosporic isolates included restriction enzyme analysis of rDNA (ARDRA) using HaeIII and HinfI, and genetic diversity was determined using Box-PCR molecular markers. Box-PCR analysis generated 14 groups. The groups correlated highly with the geographic location of the isolate and sample type. These results demonstrate the usefulness of ARDRA and Box-PCR techniques in the molecular characterization of the Fusarium genus for the discrimination of pathogenic isolates.
authors: Azul Agricultura y Servicios S.A. de C.V., Circunvalación Sur 51a, 45070, Zapopan, Jalisco, Mexico.
authors: Karla L
authors: KL
authors: Vega-Ramos
authors: J Xavier
authors: JX
authors: Uvalle-Bueno
authors: Juan F
authors: JF
authors: Gómez-Leyva
mesh_terms: D027882:Agave / Q000235:genetics / Q000382:microbiology*; D005670:Fusarium / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D014644:Genetic Variation; D010935:Plant Diseases / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology; D012988:Soil Microbiology; D013172:Spores, Fungal / Q000235:genetics