title: Improvement of the enzyme performance of trypsin via adsorption in mesoporous silica SBA-15: hydrolysis of BAPNA.
abstract: The enzymatic performance of trypsin in hydrolysis of N-α-benzoyl-DL-arginine-4-nitroanilide (BAPNA) was improved by adsorption on Santa Barbara Amorphous (SBA)-15 mesoporous silica. The optimal immobilization conditions were screened and the properties of immobilized enzyme have also been studied. Under the optimal conditions, the immobilized trypsin displays maximum specific activity (49.8 μmol/min/g). The results also indicate that the immobilized trypsin exhibits better storage stability.
authors: Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, China.
authors: Shanshan
authors: S
authors: Li
authors: Zhuofu
authors: Z
authors: Wu
authors: Ming
authors: M
authors: Lu
authors: Zhi
authors: Z
authors: Wang
authors: Zhengqiang
authors: Z
authors: Li
mesh_terms: D000327:Adsorption; D000818:Animals; D001586:Benzoylarginine Nitroanilide / Q000737:chemistry*; D055162:Biocatalysis; D002417:Cattle; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D010179:Pancreas / Q000201:enzymology; D059748:Proteolysis; D053838:Scattering, Small Angle; D012822:Silicon Dioxide / Q000737:chemistry*; D014357:Trypsin / Q000737:chemistry*; D014961:X-Ray Diffraction; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; C509969:SBA-15; D047392:beta-Cyclodextrins; D012822:Silicon Dioxide; D001586:Benzoylarginine Nitroanilide; D014357:Trypsin