title: Function of wheat phosphate transporter gene TaPHT2;1 in Pi translocation and plant growth regulation under replete and limited Pi supply conditions.
abstract: Several phosphate transporters (PTs) that belong to the Pht2 family have been released in bioinformatics databases, but only a few members of this family have been functionally characterized. In this study, we found that wheat TaPHT2;1 shared high identity with a subset of Pht2 in diverse plants. Expression analysis revealed that TaPHT2;1 was strongly expressed in the leaves, was up-regulated by low Pi stress, and exhibited a circadian rhythmic expression pattern. TaPHT2;1-green fluorescent protein fusions in the leaves of tobacco and wheat were specifically detected in the chloroplast envelop. TaPHT2;1 complemented the Pi transporter activities in a yeast mutant with a defect in Pi uptake. Knockdown expression of TaPHT2;1 significantly reduced Pi concentration in the chloroplast under sufficient (2 mM Pi) and deficient Pi (100 μM Pi) conditions, suggesting that TaPHT2;1 is crucial in the mediation of Pi translocation from the cytosol to the chloroplast. The down-regulated expression of TaPHT2;1 resulted in reduced photosynthetic capacities, total P contents, and accumulated P amounts in plants under sufficient and deficient Pi conditions, eventually leading to worse plant growth phenotypes. The TaPHT2;1 knockdown plants exhibited pronounced decrease in accumulated phosphorus in sufficient and deficient Pi conditions, suggesting that TaPHT2;1 is an important factor to associate with a distinct P signaling that up-regulates other PT members to control Pi acquisition and translocation within plants. Therefore, TaPHT2;1 is a key member of the Pht2 family involved in Pi translocation, and that it can function in the improvement of phosphorus usage efficiency in wheat.
authors: College of Agronomy, Agricultural University of Hebei, Baoding, 071001, China.
authors: Chengjin
authors: C
authors: Guo
authors: Xiaolei
authors: X
authors: Zhao
authors: Xiaoman
authors: X
authors: Liu
authors: Lijun
authors: L
authors: Zhang
authors: Juntao
authors: J
authors: Gu
authors: Xiaojuan
authors: X
authors: Li
authors: Wenjing
authors: W
authors: Lu
authors: Kai
authors: K
authors: Xiao
mesh_terms: D018533:Biomass; D002736:Chloroplasts / Q000378:metabolism; D055785:Gene Knockdown Techniques; D017343:Genes, Plant; D005816:Genetic Complementation Test; D010641:Phenotype; D028061:Phosphate Transport Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism*; D010788:Photosynthesis; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011523:Protoplasts / Q000378:metabolism; D014908:Triticum / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development
chemical_list: D028061:Phosphate Transport Proteins; D010710:Phosphates; D010940:Plant Proteins