title: [Metabolomics research on focal cerebral ischemia reperfusion injury in rats' brain treated by musk combined with borneol].
abstract: OBJECTIVE
  To study the metabolomics of focal cerebral ischemia reperfusion injury in rats' brain treated by musk combined with borneol.
  
  
  METHODS
  Amino acids, fatty acids, organic acids, glucose,and sterols were transferred to ethers or esters which were heat stable and evaporated easily. The chromatographic conditions were optimized to analyze the endogenous metabolites from serum of the rats.
  
  
  RESULTS
  The metabolic fingerprints of the endogenous metabolites were obtained by the optimum method. Twenty-nine chromatographic peaks in the metabolic fingerprints were identified by mass data and standard references. And the difference between normal, model rats, and model rats treated by musk with borneol was analyzed by the Principal Component Analysis (PCA).
  
  
  CONCLUSIONS
  The metabolic fingerprints based on the derivation reaction combined with GC-MS could analyze the endogenous metabolites; The variation between different groups was related to the quantity of the biomarkers which could be in accordance with focal cerebral ischemia reperfusion injury.
authors: The First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China.
authors: Huan
authors: H
authors: Li
authors: Xin-Hua
authors: XH
authors: Xia
authors: Mei
authors: M
authors: Liu
authors: Zhi-Min
authors: ZM
authors: Zhang
authors: Xiao-Li
authors: XL
authors: Wan
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001774:Blood Chemical Analysis; D002545:Brain Ischemia / Q000378:metabolism* / Q000517:prevention & control; D001892:Camphanes / Q000494:pharmacology*; D004195:Disease Models, Animal; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D055432:Metabolomics / Q000379:methods*; D025341:Principal Component Analysis; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism* / Q000517:prevention & control
chemical_list: D000596:Amino Acids; D015415:Biomarkers; D001892:Camphanes; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; C008563:musk; D005947:Glucose