title: Quantitative proteomic study identified cathepsin B associated with doxorubicin-induced damage in H9c2 cardiomyocytes.
abstract: The study was performed to analyze the proteomic profiling of doxorubicin-treated H9c2 cardiomyocytes in order to identify novel protein biomarkers associated with doxorubicin-induced cardiomyopathy. The protein profiling of H9c2 cells in response to doxorubicin at an apoptosis-induced concentration of 0.5 μM were compared using iTRAQ analysis. Western-blot analysis was used to confirm differentially expressed proteins identified in the proteomic study. A total of 22 differently expressed proteins were identified in doxorubicin-treated H9c2 cells including 15 up-regulated and 7 down-regulated proteins. Gene Ontology (GO) analysis revealed that 10 altered proteins were enriched in the process of apoptosis. We further validated the expression of cathepsin B and its possible regulator nuclear factor kappa B (NF-κB) in H9c2 cells were increased during doxorubicin treatment using Western-blots. Differentially expressed proteins might provide clues to clarify novel mechanisms underlying doxorubicin-induced cardiomyopathy. Our results also suggest that increased cathepsin B expression might be associated with NF-κB up-regulation, and the exact mechanisms need to be clarified.
authors: Department of Laboratory Medicine, the First people's Hospital of Yangzhou, Jiangsu, China.
authors: G Y
authors: GY
authors: Bao
authors: H Z
authors: HZ
authors: Wang
authors: Y J
authors: YJ
authors: Shang
authors: H J
authors: HJ
authors: Fan
authors: M L
authors: ML
authors: Gu
authors: R
authors: R
authors: Xia
authors: Q
authors: Q
authors: Qin
authors: A M
authors: AM
authors: Deng
mesh_terms: D000818:Animals; D002401:Cathepsin B / Q000378:metabolism*; D002460:Cell Line; D004317:Doxorubicin / Q000494:pharmacology*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D051381:Rats
chemical_list: D016328:NF-kappa B; D004317:Doxorubicin; D002401:Cathepsin B