title: [Detecting the cytotoxicities of five bisphenol A analogues to the MCF-7 human breast carcinoma cell line through different endpoints].
abstract: As the main synthetic raw materials of polycarbonate, bisphenol A (BPA) and its analogues have been important issues in environmental pollution. The current studies focus mainly on BPA's estrogen effects and little on their cytotoxic effects. To assess the cytotoxicities of the five BPA analogues, we employed the MTS assay to determine the inhibition toxicity to MCF-7 (ER-), 2,4-dinitrophenylhydrazine assay to determine the release rate of lactate dehydrogenase (LDH) escaping into cell culture medium, and single cell gel electrophoresis assay (SCGE) to detect DNA damage. The dose-response curves (DRC) between the observed inhibition toxicities and concentrations of the BPA compounds in MTS assay were fitted by using the nonlinear least squares (NLS) and the results showed that all the dose-response relationships were effectively described by the Weibull or Logit function. The toxicities expressed by--lgpEC50 were BPB > BPC > TDP > BPE > BPA. LDH assay and SCGE assay showed that when the concentrations of BPA analogues were EC20, the MCF-7 cell proliferation was slightly inhibited due to its little damaged DNA, and at EC40 the cell proliferations were significantly inhibited due to the seriously damaged DNA, leading to the damage of cell membrane and release of LDH.
authors: Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, College of Life Science, Southwest University, Chongqing 400715, China. zhang3175pm@163.com
authors: Shuai-Shuai
authors: SS
authors: Zhang
authors: Yan
authors: Y
authors: Liu
authors: Shu-Shen
authors: SS
authors: Liu
authors: Xiang-Wei
authors: XW
authors: Zhu
mesh_terms: D001559:Benzhydryl Compounds / Q000737:chemistry / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects*; D004785:Environmental Pollutants / Q000633:toxicity*; D006801:Humans; D061986:MCF-7 Cells; D010636:Phenols / Q000737:chemistry / Q000633:toxicity*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004785:Environmental Pollutants; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds