title: A novel platform for enhanced biosensing based on the synergy effects of electrospun polymer nanofibers and graphene oxides.
abstract: A novel biosensing platform was developed by combining the advantages of electrospun poly(vinyl alcohol) (PVA)/chitosan nanofibers and graphene oxides (GO). Glucose oxidase (GOD) was employed as a model enzyme. By co-electrospinning the solution of PVA, chitosan, GOD and GO, the PVA/chitosan/GOD/GO nanofibers were directly modified on the platinum (Pt) electrode. The UV-vis spectra and the FTIR spectra were used to characterize the GO nanosheets. The morphologies of fabricated electrospun nanofibers were characterized by high resolution scanning electron microscopy. After a thin layer of nafion was modified on the surface of matrix, the as-prepared electrode was used to detect glucose. The electrode exhibited great advantages in high sensitivity, low detection limit and wide linear range. In the meantime, the electrode showed good stability, acceptable reproducibility, and excellent anti-interference capability for ascorbic acid, uric acid, lactose and sucrose. Moreover, the novel biosensor was successfully applied for the glucose determination in human serum samples. The mechanism of efficient biosensing of the nafion/PVA/chitosan/GOD/GO/Pt electrode was analyzed in detail and the results show that it can be due to the synergy effects of electrospun nanofibers and GO nanosheets.
authors: Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors: Xiaofang
authors: X
authors: Su
authors: Jun
authors: J
authors: Ren
authors: Xianwei
authors: X
authors: Meng
authors: Xiangling
authors: X
authors: Ren
authors: Fangqiong
authors: F
authors: Tang
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis*; D048271:Chitosan / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D008958:Models, Molecular; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010087:Oxides / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D001786:Blood Glucose; D004800:Enzymes, Immobilized; D010087:Oxides; D006108:Graphite; D011142:Polyvinyl Alcohol; D048271:Chitosan; D005949:Glucose Oxidase