title: The protective effects of sildenafil in acute lung injury in a rat model of severe scald burn: A biochemical and histopathological study.
abstract: Severe burn induces biochemical mediators such as reactive oxygen species that leads to lipid peroxidation which may have a key role in formation of acute lung injury (ALI). Sildenafil is a selective and potent inhibitor of cyclic guanosine monophosphate specific phosphodiesterase-5. Sildenafil preserves alveolar growth, angiogenesis, reduces inflammation and airway reactivity. The purpose of the present study was to evaluate the effects of different dosages of sildenafil in ALI due to severe scald burn in rats. Twenty-four rats were subjected to 30% total body surface area severe scald injury and were randomly divided into three equal groups as follow: control, 10 and 20mg/kg sildenafil groups. Levels of malondialdehyde (MDA), activities of glutathione peroxidase (Gpx), catalase (Cat), total oxidative stress (TOS), and total antioxidative capacity (TAC) were measured in both tissues and serums. Oxidative stress index (OSI) was calculated. A semi-quantitative scoring system was used for the evaluation of histopatological findings. Sildenafil increased Gpx, Cat, TAC and decreased MDA, TOS and OSI. Sildenafil decreased inflammation scores in lungs. Our results reveal that sildenafil is protective against scald burn related ALI by decreasing oxidative stress and inflammation and the dosage of 10mg/kg could be apparently better than 20mg/kg.
authors: Cumhuriyet University School of Medicine, Department of General Surgery, Sivas, Turkey. dralihan20@hotmail.com
authors: Ali Kagan
authors: AK
authors: Gokakin
authors: Koksal
authors: K
authors: Deveci
authors: Atilla
authors: A
authors: Kurt
authors: Boran Cihat
authors: BC
authors: Karakus
authors: Cevdet
authors: C
authors: Duger
authors: Mehmet
authors: M
authors: Tuzcu
authors: Omer
authors: O
authors: Topcu
mesh_terms: D055371:Acute Lung Injury / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002056:Burns / Q000150:complications*; D002374:Catalase / Q000032:analysis; D004195:Disease Models, Animal; D005260:Female; D005979:Glutathione Peroxidase / Q000032:analysis; D008315:Malondialdehyde / Q000032:analysis; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D058986:Phosphodiesterase 5 Inhibitors / Q000627:therapeutic use*; D010879:Piperazines / Q000627:therapeutic use*; D011687:Purines / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D000068677:Sildenafil Citrate; D013450:Sulfones / Q000627:therapeutic use*
chemical_list: D015415:Biomarkers; D058986:Phosphodiesterase 5 Inhibitors; D010879:Piperazines; D011687:Purines; D013450:Sulfones; D008315:Malondialdehyde; D000068677:Sildenafil Citrate; D002374:Catalase; D005979:Glutathione Peroxidase
keywords: Acute lung injury; Burn; Sildenafil