title: Design of a multiplex PCR method for detection of toxigenic-pathogenic in Vibrio cholerae.
abstract: OBJECTIVE
  To study virulence and regulatory genes (hlyA, ctxB, tcpI) in clinical strains of Vibrio cholerae (V. cholerae), simultaneously.
  
  
  METHODS
  Three important genes, tcpI, hlyA and ctxB were used for detection of toxigenic and pathogenic V. cholera by chain reaction assay method.
  
  
  RESULTS
  According to the results of the PCR, the incidence of hlyA, tcpI, and ctxB genes in clinical isolates was obtained as 94.7% (72 sample), 90.8% (69 sample), and 92.1% (70 sample), respectively. Five strains possessed all genes except ctxB, six strains possessed all genes except tcpI, four strains possessed all genes except hlyA, one strain possessed only hlyA and 60 strains contained a combination of three genes, Including hlyA, ctxB and tcpI.
  
  
  CONCLUSIONS
  Result show that this method could be reliable to detect toxigenic-pathogenic strains of V. cholerae in Iran.
authors: Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. imanifouladi.a@bmsu.ac.ir
authors: Fooladi A A
authors: FA
authors: Imani
authors: Islamieh D
authors: ID
authors: Iman
authors: Doust R
authors: DR
authors: Hosseini
authors: A
authors: A
authors: Karami
authors: S M
authors: SM
authors: Marashi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D002771:Cholera / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005243:Feces / Q000382:microbiology; D005798:Genes, Bacterial / Q000235:genetics; D006460:Hemolysin Proteins / Q000235:genetics; D006801:Humans; D007492:Iran; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D012097:Repressor Proteins / Q000235:genetics; D012680:Sensitivity and Specificity; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D006460:Hemolysin Proteins; D012097:Repressor Proteins; C097245:TcpI protein, Vibrio cholerae; C084287:hlyA protein, Vibrio cholerae