title: Determination of antibodies against human growth hormone using a direct immunoassay format and different electrochemical methods.
abstract: A direct immunoassay format with human growth hormone (hGH) immobilized on a self assembled monolayer modified surface plasmon resonance (SPR) chip was chosen to detect specific antibodies (anti-hGH) using different electrochemical techniques. Atomic force microscopy imaging and SPR were used as control methods for the evaluation and confirmation of the antigen-antibody complex formation. The applicability and sensitivity of candidate electrochemical techniques to develop an accurate and sensitive electrochemical immunosensor were investigated. Four electrochemical methods for anti-hGH determination - pulse amperometry (PA), cyclic voltammetry (CV), square wave voltammetry (SWV) and differential pulse voltammetry (DPV), were compared. Higher sensitivity of the developed immunosensor was observed using PA and CV: analytical signal registered using the PA method was 2.50 times higher in comparison with CV, 16.3 times higher in comparison with SWV and 24.5 times higher in comparison with the DPV method. In the case of PA detection method, the limit of detection was lower (75 nM) than that of the CV method (108 nM).
authors: Division of Immunotechnology, State Research Institute Centre for Innovative Medicine, Zygimantu 9, LT-01102 Vilnius, Lithuania.
authors: Natalija
authors: N
authors: German
authors: Asta
authors: A
authors: Kausaite-Minkstimiene
authors: Justina
authors: J
authors: Kirlyte
authors: Asta
authors: A
authors: Makaraviciute
authors: Arunas
authors: A
authors: Ramanavicius
authors: Lina
authors: L
authors: Mikoliunaite
authors: Almira
authors: A
authors: Ramanaviciene
mesh_terms: D000936:Antigen-Antibody Complex / Q000032:analysis* / Q000276:immunology; D055664:Electrochemical Techniques / Q000379:methods*; D019382:Human Growth Hormone / Q000276:immunology*; D006801:Humans; D055767:Immobilized Proteins / Q000276:immunology; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D018625:Microscopy, Atomic Force; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D000936:Antigen-Antibody Complex; D055767:Immobilized Proteins; D019382:Human Growth Hormone