title: A new cancer cell detection method using an infectivity-enhanced adenoviral vector.
abstract: Cytological examination is widely used as a diagnostic tool because of the ease of collecting cells from the involved area. However, the diagnostic yield of cytological examination is unsatisfactory; the reasons include sampling error, poorly prepared samples, small numbers of malignant cells, and low grades of cellular atypia. In this study, we focused on the high infectivity of adenovirus towards epithelial cells and applied the luciferase- expressing adenoviral vector to a new cancer cell detection tool. In addition, adenoviral infectivity was enhanced by modifying viral fiber proteins. The sensitivity of the diagnostic tool was tested using the NCI-H1299 lung cancer cell line, and validated in body fluid samples from cancer patients with a variety of etiology. Results showed that the adenovirus efficiently transfected NCI-H1299 with high sensitivity. Only 10 cancer cells were sufficient for detection of luciferase signals. In body fluid samples, the adenovirus confirmed the diagnosis for malignant and benign cancer, but not in non-epithelial cell derived samples. This study provides proof-of-concept for a more reliable and sensitive diagnostic tool for epithelium-derived cancer.
authors: Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Japan.
authors: Junji
authors: J
authors: Uchino
authors: Koichi
authors: K
authors: Takayama
authors: Noriaki
authors: N
authors: Nakagaki
authors: Wang
authors: W
authors: Shuo
authors: Junko
authors: J
authors: Hisasue
authors: Keita
authors: K
authors: Nakatom
authors: Keiichi
authors: K
authors: Ohta
authors: Ryosuke
authors: R
authors: Hirano
authors: Naoki
authors: N
authors: Tashiro
authors: Izumi
authors: I
authors: Miiru
authors: Masaki
authors: M
authors: Fujita
authors: Kentaro
authors: K
authors: Watanabe
authors: Yoichi
authors: Y
authors: Nakanishi
mesh_terms: D000256:Adenoviridae / Q000472:pathogenicity*; D000257:Adenoviridae Infections / Q000175:diagnosis* / Q000378:metabolism / Q000821:virology; D002478:Cells, Cultured; D005260:Female; D005822:Genetic Vectors / Q000008:administration & dosage; D006801:Humans; D007668:Kidney / Q000378:metabolism / Q000821:virology; D008156:Luciferases / Q000378:metabolism; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000821:virology; D008297:Male; D009375:Neoplasms, Glandular and Epithelial / Q000175:diagnosis* / Q000378:metabolism / Q000821:virology; D016066:Pleural Effusion, Malignant / Q000175:diagnosis* / Q000378:metabolism / Q000821:virology; D011379:Prognosis
chemical_list: D008156:Luciferases