title: Identification of gene clusters associated with host adaptation and antibiotic resistance in Chinese Staphylococcus aureus isolates by microarray-based comparative genomics.
abstract: A comparative genomic microarray comprising 2,457 genes from two whole genomes of S. aureus was employed for the comparative genome hybridization analysis of 50 strains of divergent clonal lineages, including methicillin-resistant S. aureus (MRSA), methicillin-susceptible S. aureus (MSSA), and swine strains in China. Large-scale validation was confirmed via polymerase chain reaction in 160 representative clinical strains. All of the 50 strains were clustered into seven different complexes by phylogenetic tree analysis. Thirteen gene clusters were specific to different S. aureus clones. Ten gene clusters, including seven known (vSa3, vSa4, vSaα, vSaβ, Tn5801, and phage ϕSa3) and three novel (C8, C9, and C10) gene clusters, were specific to human MRSA. Notably, two global regulators, sarH2 and sarH3, at cluster C9 were specific to human MRSA, and plasmid pUB110 at cluster C10 was specific to swine MRSA. Three clusters known to be part of SCCmec, vSa4 or Tn5801, and vSaα as well as one novel gene cluster C12 with homology with Tn554 of S. epidermidis were identified as MRSA-specific gene clusters. The replacement of ST239-spa t037 with ST239-spa t030 in Beijing may be a result of its acquisition of vSa4, phage ϕSa1, and ϕSa3. In summary, thirteen critical gene clusters were identified to be contributors to the evolution of host specificity and antibiotic resistance in Chinese S. aureus.
authors: Department of Clinical Laboratory, Peking University People's Hospital, Beijing, People's Republic of China.
authors: Henan
authors: H
authors: Li
authors: Chunjiang
authors: C
authors: Zhao
authors: Hongbin
authors: H
authors: Chen
authors: Feifei
authors: F
authors: Zhang
authors: Wenqiang
authors: W
authors: He
authors: Xiaojuan
authors: X
authors: Wang
authors: Qi
authors: Q
authors: Wang
authors: Ruifu
authors: R
authors: Yang
authors: Dongsheng
authors: D
authors: Zhou
authors: Hui
authors: H
authors: Wang
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D000818:Animals; D002681:China; D002999:Clone Cells; D055028:Comparative Genomic Hybridization; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects / Q000235:genetics*; D022761:Gene Transfer, Horizontal; D023281:Genomics; D058507:Host Specificity; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification* / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D005810:Multigene Family; D010802:Phylogeny; D058974:Phylogeography; D010957:Plasmids; D013045:Species Specificity; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D013212:Staphylococcus epidermidis / Q000235:genetics; D013552:Swine / Q000382:microbiology