title: Pontibacter ramchanderi sp. nov., isolated from hexachlorocyclohexane-contaminated pond sediment.
abstract: A Gram-stain-negative, motile, red pigmented, rod-shaped bacterium, designated strain LP43(T), was isolated from hexachlorocyclohexane (HCH)-contaminated soil sediment (Lucknow, India). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate formed a cluster with the genus Pontibacter in the phylum Bacteroidetes with sequence similarities ranging from 92.9 to 97.0 % with species of the genus Pontibacter. The DNA G+C content of strain LP43(T) was 59.1 mol%. The polar lipid profile of strain LP43(T) showed the presence of phosphatidylethanolamine, an unidentified aminophospholipid, unknown aminolipids and unknown polar lipids. Strain LP43(T) contained MK-7 as the predominant menaquinone and sym-homospermidine as the major polyamine. The major cellular fatty acids of strain LP43(T) were, iso-C15 : 0 (15.74 %), iso-C15 : 0 3-OH (7.57 %), iso-C17 : 0 3-OH (7.32 %), summed feature 4 (iso-C17 : 1 I/anteiso-C17 : 1 B) (31.22 %) and summed feature 8 (C18 : 1ω7c/ C18 : 1ω6c) (7.60 %). Based on the results of DNA-DNA hybridization and phenotypic and genotypic characteristics, strain LP43(T) represents a novel species of the genus Pontibacter, for which the name Pontibacter ramchanderi is proposed. The type strain is LP43(T) (= CCM 8406(T) = MCC 2019(T)).
authors: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110007, India.
authors: Amit Kumar
authors: AK
authors: Singh
authors: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110007, India.
authors: Nidhi
authors: N
authors: Garg
authors: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110007, India.
authors: Naseer
authors: N
authors: Sangwan
authors: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110007, India.
authors: Vivek
authors: V
authors: Negi
authors: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110007, India.
authors: Roshan
authors: R
authors: Kumar
authors: IMTECH-Institute of Microbial Technology, Sector-39A, Chandigarh-160036, India.
authors: Surendra
authors: S
authors: Vikram
authors: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi-110007, India.
authors: Rup
authors: R
authors: Lal
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D001556:Hexachlorocyclohexane; D007194:India; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D061005:Ponds / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012989:Soil Pollutants; D013095:Spermidine / Q000031:analogs & derivatives / Q000032:analysis; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; C007961:sym-homospermidine; D024482:Vitamin K 2; D001556:Hexachlorocyclohexane; C062629:menaquinone 7; D013095:Spermidine