title: Highly lymphatic metastatic pancreatic cancer cells possess stem cell-like             properties.
abstract: Cancer stem cells are thought to be the origin of tumor metastasis. However,             evidence of cancer stem cells as the source of lymphatic metastasis in pancreatic             cancer is not clear. In this study, we examined the stem cell-like properties             of the highly lymphatic metastatic pancreatic cancer cells BxPC-3-LN. Compared             with the parental BxPC-3 cells, the BxPC-3-LN cells showed stem cell-like properties,             including high lymphatic metastasis potential, self-renewal ability and chemoresistance.             In addition, the BxPC-3-LN cells also expressed higher levels of sonic hedgehog             and migrating cancer stem cell surface markers (CD133 and CXCR4) compared to the             parental BxPC-3 cells. The growth of BxPC-3-LN cells was significantly inhibited             by gemcitabine combined with the sonic hedgehog inhibitor cyclopamine. The BxPC-3-LN             cells expressed lower levels of let-7, miR-34, miR-107, miR-125, miR-128, miR-130,             miR-132 and miR-141 than the parental BxPC-3 cells detected by microRNA PCR array,             which were reported to have close relation to stem cell factors. This study provides             evidence that cancer stem cells are the major sources of pancreatic cancer lymphatic             metastasis, and microRNAs may regulate lymphatic metastasis in pancreatic cancer             through modulating cancer stem cells.
authors: Department of Pancreatic and Hepatobiliary Surgery, Fudan University Shanghai Cancer Center, Shanghai Medical College, Fudan University, Shanghai 200032, P.R. China.
authors: Guopei
authors: G
authors: Luo
authors: Jiang
authors: J
authors: Long
authors: Xiaobo
authors: X
authors: Cui
authors: Zhiwen
authors: Z
authors: Xiao
authors: Zuqiang
authors: Z
authors: Liu
authors: Si
authors: S
authors: Shi
authors: Liang
authors: L
authors: Liu
authors: Chen
authors: C
authors: Liu
authors: Jin
authors: J
authors: Xu
authors: Min
authors: M
authors: Li
authors: Xianjun
authors: X
authors: Yu
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003841:Deoxycytidine / Q000031:analogs & derivatives / Q000494:pharmacology; D006023:Glycoproteins / Q000096:biosynthesis; D053823:Hedgehog Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000378:metabolism*; D010455:Peptides; D019718:Receptors, CXCR4 / Q000096:biosynthesis; D014704:Veratrum Alkaloids / Q000494:pharmacology; D000093542:Gemcitabine
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D000964:Antimetabolites, Antineoplastic; C527276:CXCR4 protein, human; D006023:Glycoproteins; D053823:Hedgehog Proteins; D035683:MicroRNAs; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse; D019718:Receptors, CXCR4; C509239:SHH protein, human; D014704:Veratrum Alkaloids; D003841:Deoxycytidine; C000541:cyclopamine; D000093542:Gemcitabine