title: Correlation of peroxisome proliferator-activated receptor (PPAR-γ) mRNA expression with Pro12Ala polymorphism in obesity.
abstract: Our study aimed to analyze whether the expression of PPARγ mRNA in subcutaneous adipocyte tissue correlates with Pro12Ala PPARγ2 polymorphism in the obesity context. We found that mRNA expression of PPARγ in subcutaneous adipose tissue was greater in obese subjects (P < 0.05) than in the nonobese control group. Concurrently, genotyping of the Pro12Ala polymorphism showed that obese subjects possess a significantly higher frequency of the Pro/Pro genotype than nonobese controls (90.5 vs 79.5%; P = 0.03), suggesting that this genotype is involved in an increased risk of obesity in the Tunisian population. Taken together, our results demonstrate that the Pro12 allele is accompanied by an overexpression of PPARγ mRNA in subcutaneous adipocyte tissue, suggesting that the PPARγ Pro12Ala variant may contribute to the observed variability in PPARγ mRNA expression and consequently in body mass index and insulin sensitivity in the general population.
authors: Laboratory of Genetics, Immunology and Human Pathologies, Faculty of Sciences of Tunis, University of El Manar 1, 2092, Tunis, Tunisia. berhoumar@gmail.com
authors: Rym
authors: R
authors: Berhouma
authors: Soumaya
authors: S
authors: Kouidhi
authors: Myriam
authors: M
authors: Ammar
authors: Hafawa
authors: H
authors: Abid
authors: Hajer
authors: H
authors: Ennafaa
authors: Amel
authors: A
authors: Benammar-Elgaaied
mesh_terms: D000328:Adult; D015992:Body Mass Index; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000235:genetics* / Q000378:metabolism; D047495:PPAR gamma / Q000096:biosynthesis / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis*
chemical_list: D047495:PPAR gamma; D012333:RNA, Messenger