title: rBC2LCN, a new probe for live cell imaging of human pluripotent stem cells.
abstract: Cell surface biomarkers have been applied to discriminate pluripotent human embryonic stem cells and induced pluripotent stem cells from differentiated cells. Here, we demonstrate that a recombinant lectin probe, rBC2LCN, a new tool for fluorescence-based imaging and flow cytometry analysis of pluripotent stem cells, is an alternative to conventional pluripotent maker antibodies. Live or fixed colonies of both human embryonic stem cells and induced pluripotent stem cells were visualized in culture medium containing fluorescent dye-labeled rBC2LCN. Fluorescent dye-labeled rBC2LCN was also successfully used to separate live pluripotent stem cells from a mixed cell population by flow cytometry.
authors: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan.
authors: Yasuko
authors: Y
authors: Onuma
authors: Hiroaki
authors: H
authors: Tateno
authors: Jun
authors: J
authors: Hirabayashi
authors: Yuzuru
authors: Y
authors: Ito
authors: Makoto
authors: M
authors: Asashima
mesh_terms: D015415:Biomarkers / Q000032:analysis; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D053595:Embryonic Stem Cells; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D057026:Induced Pluripotent Stem Cells; D037102:Lectins / Q000737:chemistry*; D057054:Molecular Imaging; D015335:Molecular Probes / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D005456:Fluorescent Dyes; D037102:Lectins; D015335:Molecular Probes; D011994:Recombinant Proteins