title: Effects of hypoxia/ischemia on catabolic mediators of cartilage in a human chondrocyte, SW1353.
abstract: Cells from a human chondrocyte cell line were studied in 1% oxygen and/or a lower glucose concentration (5.5 mM), compared to the routine culture conditions of normoxia and high glucose. HIF-1α, IL-1β, IL-6, IL-8, COX-2, TNFα, LIF, MMP-3, MMP-13, and reactive oxygen species (ROS) were evaluated, respectively. Effects of hypoxia inducing expression of HIF-1α were statistically significant at 72 h (p<0.05). Increased production of ROS by hypoxia was also observed with passage of time (p<0.05). The effects of hypoxia on HIF-1α and IL-1β were potentiated by 5.5 mM glucose, especially after 48 h (p<0.05). IL-8 production was significantly induced in 1% O(2), with 5.5 mM glucose (p<0.01). IL-8 mRNA expression and production in response to IL-1β were potentiated by hypoxia/ischemia (p<0.05, p<0.01, respectively). Up-regulation of IL-1β, ROS, and IL-8 by hypoxia/ischemia in human chondrocytes may occur in correlation with HIF-1α. IL-8 response to IL-1β may be potentiated synergically by hypoxia/ischemia, as an effector of hypoxia/ischemia. The results may suggest aggressive biology of the ordinary cartilage hypoxia/ischemia in the context of arthro-degeneration.
authors: Department of Internal Medicine, School of Medicine, Yeungnam University, Daegu, South Korea. yhhong@med.yu.ac.kr
authors: Young Hoon
authors: YH
authors: Hong
authors: Chan Won
authors: CW
authors: Park
authors: Hee Sun
authors: HS
authors: Kim
authors: Kyu Chang
authors: KC
authors: Won
authors: Yong Woon
authors: YW
authors: Kim
authors: Choong Ki
authors: CK
authors: Lee
mesh_terms: D002358:Cartilage, Articular / Q000098:blood supply* / Q000378:metabolism* / Q000473:pathology; D002460:Cell Line; D019902:Chondrocytes / Q000378:metabolism* / Q000473:pathology; D005947:Glucose / Q000378:metabolism; D006801:Humans; D000860:Hypoxia / Q000378:metabolism* / Q000473:pathology; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000096:biosynthesis*; D053583:Interleukin-1beta / Q000096:biosynthesis*; D016209:Interleukin-8 / Q000096:biosynthesis*; D007511:Ischemia / Q000378:metabolism* / Q000473:pathology; D017382:Reactive Oxygen Species / Q000378:metabolism; D015854:Up-Regulation
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D053583:Interleukin-1beta; D016209:Interleukin-8; D017382:Reactive Oxygen Species; D005947:Glucose