title: [The expression and meanning of Skp2, p27, PTEN proteins in non-Hodgkin B-cell lymphoma of ocular adnexa].
abstract: OBJECTIVE
  To investigate the expression and the correlation of protein of S-phase kinase association protein 2(Skp2), p27 and phosphatases phosphatase and tensin homologue (PTEN) in non-Hodgkin B-cell lymphoma (NHL)of ocular adnexa.
  
  
  METHODS
  Experimental study. Paraffin sections were collected from patients suffering from B cell NHL (n = 30) in Qingdao Affiliated Hospital from January 1995 to January 2011 and lymphadenosis (n = 10) of ocular adnexa as the control group. The expression of Skp2, p27 and PTEN proteins were detected by immunohistochemistry and the positive expression rate between lymphomas and lymphadenosis was compared by χ(2) inspection. Spearman rank correlation was used to estimate the relationships among three proteins in ocular lymphoma. Pathologic grade were evaluated as independent factor of clinical information.
  
  
  RESULTS
  The expression of three proteins were related to pathologic type of tumors, but not related to age, gender, pathogenetic locations. In non-Hodgkin B-cell lymphoma of ocular adnexa, the expression of Skp2 (76.7%, 23/30) was higher than that in control group (0/10) (χ(2) = 19.79, P = 0.001), while the expression of p27 (73.3%, 22/30) and PTEN (56.7%, 17/30) was lower than that in the control group (10/10 and 10/10) (χ(2) = 8.37, P = 0.039;χ(2) = 13.48, P = 0.004). With the increase of pathologic grade, Skp2 labeling frequency increase gradually, while p27 and PTEN labeling frequency decrease gradually;there was a negative correlation between p27 and Skp2 in MALT (r = -0.134, P < 0.05) while there was a positive correlation between p27 and PTEN (r = 0.828, P < 0.05). The relationship between Skp2 and PTEN was also negative (r = -0.883, P < 0.05).
  
  
  CONCLUSIONS
  The higher expression of Skp2 and lower expression of p27 and PTEN may play a role in the tumorigenesis and different pathologic type of the B cell NHL of ocular adnexa. The expressions of three proteins correlate with each other in mucosa-associated lymphoid tissue.
authors: Department of Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao 266003, China.
authors: Jing-jing
authors: JJ
authors: Zhang
authors: Gui-qiu
authors: GQ
authors: Zhao
authors: Hong
authors: H
authors: Lin
authors: Xian
authors: X
authors: Yang
authors: Jing
authors: J
authors: Lin
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism*; D005134:Eye Neoplasms / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D051059:PTEN Phosphohydrolase / Q000378:metabolism*; D044786:S-Phase Kinase-Associated Proteins / Q000378:metabolism*
chemical_list: C436291:CDKN1B protein, human; D044786:S-Phase Kinase-Associated Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human