title: Sequence and immunogenicity of a clinically approved novel measles virus vaccine vector.
abstract: The measles virus vaccine (MVbv) is a clinically certified and well-tolerated vaccine strain that has been given both parenterally and mucosally. It has been extensively used in children and has proven to be safe and effective in eliciting protective immunity. This specific strain was therefore chosen to generate a measles viral vector. The genome of the commercial MVbv vaccine strain was isolated, sequenced and a plasmid, p(+)MVb, enabling transcription of the viral antigenome and rescue of MVb, was constructed. Phylogenic and phenotypic analysis revealed that MVbv and the rescued MVb constitute another evolutionary branch within the hitherto classified measles vaccines. Plasmid p(+)MVb was modified by insertion of artificial MV-type transcription units (ATUs) for the generation of recombinant viruses (rMVb) expressing additional proteins. Replication characteristics and immunogenicity of rMVb vectors were similar to the parental MVbv and to other vaccine strains. The expression of the additional proteins was stable over 10 serial virus transfers, which corresponds to an amplification greater than 10 ( 20) . The excellent safety record and its efficient application as aerosol may add to the usefulness of the derived vectors.
authors: Current affiliations: Zurich University of Applied Sciences; Wädenswil, Switzerland.
authors: Amando
authors: A
authors: Zuniga
authors: Mathias
authors: M
authors: Liniger
authors: Teldja Neige Azzouz
authors: TN
authors: Morin
authors: René R
authors: RR
authors: Marty
authors: Marian
authors: M
authors: Wiegand
authors: Orhan
authors: O
authors: Ilter
authors: Sara
authors: S
authors: Weibel
authors: Martin A
authors: MA
authors: Billeter
authors: Marlyse C
authors: MC
authors: Knuchel
authors: Hussein Y
authors: HY
authors: Naim
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D016000:Cluster Analysis; D015870:Gene Expression; D005822:Genetic Vectors; D042822:Genomic Instability; D008459:Measles virus / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010957:Plasmids; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014709:Vero Cells; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*; D014779:Virus Replication
chemical_list: D014765:Viral Vaccines
keywords: live-attenuated vaccines; recombinant measles virus; viral vectors