title: Species variation in the spontaneous calcification of bone marrow-derived mesenchymal stem cells.
abstract: Bone marrow-derived mesenchymal stem cells (BM-MSCs) hold great promise for tissue regeneration. With increasing numbers of clinical trials, the safety of BM-MSCs attracts great interest. Previously, we determined that rat BM-MSCs possessed spontaneous calcification without osteogenic induction after continuous culture. However, it is unclear whether BM-MSCs from other species share this characteristic. In this study, spontaneous calcification of BM-MSCs from rat, goat, and human specimens was investigated in vitro. BM-MSCs were cultured in complete medium, and calcification was determined by morphologic observation and alizarin red staining. It was demonstrated that rat BM-MSCs possessed a typically spontaneous calcification, whereas goat and human BM-MSCs under the same system proliferated significantly but did not calcify spontaneously. The significant species variation in spontaneous calcification of BM-MSCs described in this study provides useful information regarding evaluation of numerous BM-MSC-based approaches for bone regeneration and the safety of BM-MSCs.
authors: Laboratory of Stem Cell and Tissue Engineering, Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, PR China.
authors: Yi-Zhou
authors: YZ
authors: Huang
authors: Jia-Qin
authors: JQ
authors: Cai
authors: Feng-Juan
authors: FJ
authors: Lv
authors: Hong-Lei
authors: HL
authors: Xie
authors: Zhi-Ming
authors: ZM
authors: Yang
authors: Yong-Can
authors: YC
authors: Huang
authors: Li
authors: L
authors: Deng
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology*; D001861:Bone Regeneration; D002114:Calcinosis; D006041:Goats / Q000502:physiology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D013045:Species Specificity