title: [Significance and expression of Sirt1 gene in human cervical endplate chondrocyte in vitro degeneration].
abstract: OBJECTIVE
  To observe the expression changes of Sirt1 gene and examine the role and significance of degenerative process in human cervical endplate chondrocytes through a degeneration model of human cervical vertebral endplate chondrocyte.
  
  
  METHODS
  Cartilage endplates of 30 patients were divided into control group (n = 16) with cervical vertebral fracture or dislocation and cervical spondylosis group (n = 14) with cervical spondylotic myelopathy. Endplate chondrocytes were isolated by enzyme digestion and cultured in vitro for 10 days. The differences of endplate chondrocytes from normal and degenerative cartilage endplates were observed by inverted phase-contrast microscope, hematoxylin and eosin staining and toluidine blue staining. Real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were used to detect the mRNA expressions of Sirt1, collagen II and aggrecan.
  
  
  RESULTS
  Compared with the normal group, the cellular morphology of degenerative group showed spindle-shaped changes. The mRNA expression of Sirt1 (P = 0.034) significantly decreased. Aggrecan (P = 0.0063) and collagen II (P = 0.0072) decreased also markedly.
  
  
  CONCLUSIONS
  Sirt1 gene expression is significantly down-regulated in degenerative human cervical endplate chondrocytes. Regulating the expression of Sirt1 gene may block or delay the occurrence of human cervical endplate cartilage degeneration.
authors: Department of Orthopedic Surgery, Yijishan Hospital, Wannan Medical College, Wuhu 241001, China.
authors: Bin
authors: B
authors: Hu
authors: Hong-guang
authors: HG
authors: Xu
authors: Jun-xing
authors: JX
authors: Song
authors: Ping-zhi
authors: PZ
authors: Zhang
authors: Xiao-hai
authors: XH
authors: Zhang
authors: Jia-Feng
authors: JF
authors: Cheng
mesh_terms: D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D002574:Cervical Vertebrae / Q000166:cytology / Q000473:pathology*; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism / Q000473:pathology*; D015536:Down-Regulation; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D008875:Middle Aged; D056564:Sirtuin 1 / Q000235:genetics*; D013118:Spinal Cord Diseases / Q000473:pathology; D055009:Spondylosis / Q000473:pathology
chemical_list: C447939:SIRT1 protein, human; D056564:Sirtuin 1