title: Population genetic structure of Aedes (Stegomyia) aegypti (L.) at a micro-spatial scale in Thailand: implications for a dengue suppression strategy.
abstract: BACKGROUND
  The genetic population structure of Aedes (Stegomyia) aegypti (L.), the main vector of dengue virus, is being investigated in areas where a novel dengue suppression program is to be implemented. The aim of the program is to release and establish mosquito populations with impaired virus transmission capabilities. To model effects of the release and devise protocols for its implementation, information about the genetic structure of populations at a range of spatial scales is required.
  
  
  RESULTS
  This study investigates a potential release site in the Hua Sam Rong Subdistrict of Plaeng Yao District, Chachoengsao Province, in eastern Thailand which comprises a complex of five villages within a 10 km radius. Aedes aegypti resting indoors was sampled at four different times of year from houses within the five villages. Genetic markers were used to screen the mosquitoes: two Exon Primed Intron Crossing (EPIC) markers and five microsatellite markers. The raw allele size was determined using several statistical software packages to analyze the population structure of the mosquito. Estimates of effective population size for each village were low, but there was no evidence of genetic isolation by geographic distance.
  
  
  CONCLUSIONS
  The presence of temporary genetic structure is possibly caused by genetic drift due to large contributions of adults from a few breeding containers. This suggests that the introduction of mosquitoes into an area needs to proceed through multiple releases and targeting of sites where mosquitoes are emerging in large numbers.
authors: Centre of Excellence for Vectors and Vector-Borne Diseases, Faculty of Science, Mahidol University at Salaya, Nakhon Pathom, Thailand.
authors: Phanthip
authors: P
authors: Olanratmanee
authors: Pattamaporn
authors: P
authors: Kittayapong
authors: Chitti
authors: C
authors: Chansang
authors: Ary A
authors: AA
authors: Hoffmann
authors: Andrew R
authors: AR
authors: Weeks
authors: Nancy M
authors: NM
authors: Endersby
mesh_terms: D000330:Aedes / Q000145:classification / Q000235:genetics / Q000502:physiology*; D000818:Animals; D003715:Dengue / Q000453:epidemiology / Q000517:prevention & control; D005819:Genetic Markers; D014644:Genetic Variation; D006801:Humans; D007303:Insect Vectors; D009032:Mosquito Control / Q000379:methods; D013785:Thailand / Q000453:epidemiology
chemical_list: D005819:Genetic Markers