title: Novel Nanodimension artificial red blood cells that act as O2 and CO2 carrier with enhanced antioxidant activity: PLA-PEG nanoencapsulated PolySFHb-superoxide dismutase-catalase-carbonic anhydrase.
abstract: Poly(ethylene glycol)-Poly(lactic acid) block-copolymer (PEG-PLA) was prepared and characterized using Fourier transform infrared spectrophotometer (FTIR). Glutaraldehyde was used to crosslink stroma-free hemoglobin (SFHb), superoxide dismutase (SOD), catalase (CAT), and carbonic anhydrase (CA) into a soluble complex of PolySFHb-SOD-CAT-CA. PEG-PLA was then used to nanoencapsulated PolySFHb-SOD-CAT-CA by oil in water emulsification. This resulted in the formation of PLA-PEG-PolySFHb-SOD-CAT-CA nanocapsules that have enhanced antioxidant activity and that can transport both O2 and CO2. These are homogeneous particles with an average diameter of 100 nm with good dispersion and core shell structure, high entrapment efficiency (EE%), and nanocapsule percent recovery. A lethal hemorrhagic shock model in rats was used to evaluate the therapeutic effect of the PLA-PEG-PolySFHb-SOD-CAT-CA nanocapsules. Infusion of this preparation resulted in the lowering of the elevated tissue PCO2 and also recovery of the mean arterial pressure (MAP).
authors: Departments of Physiology, Medicine and Biomedical Engineering, Faculty of Medicine, Artificial Cells and Organs Research Center, McGill University, Montreal, QC, Canada.
authors: Wei
authors: W
authors: Gao
authors: Yuzhu
authors: Y
authors: Bian
authors: Thomas M S
authors: TM
authors: Chang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism* / Q000627:therapeutic use; D001802:Blood Substitutes / Q000737:chemistry / Q000378:metabolism* / Q000627:therapeutic use; D002214:Capsules; D002245:Carbon Dioxide / Q000378:metabolism*; D002256:Carbonic Anhydrases / Q000737:chemistry / Q000378:metabolism; D002374:Catalase / Q000737:chemistry / Q000378:metabolism; D006454:Hemoglobins / Q000737:chemistry*; D007773:Lactates / Q000737:chemistry*; D008297:Male; D049329:Nanostructures / Q000737:chemistry*; D010100:Oxygen / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012771:Shock, Hemorrhagic / Q000628:therapy; D013482:Superoxide Dismutase / Q000737:chemistry / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001802:Blood Substitutes; D002214:Capsules; D006454:Hemoglobins; D007773:Lactates; C016484:hemoglobin, stroma free; C088513:poly(lactic acid-ethylene glycol); D002245:Carbon Dioxide; D011092:Polyethylene Glycols; D002374:Catalase; D013482:Superoxide Dismutase; D002256:Carbonic Anhydrases; D010100:Oxygen