title: Spatial mismatch between the Na+ flux and spike initiation in axon initial segment.
abstract: It is widely believed that, in cortical pyramidal cells, action potentials (APs) initiate in the distal portion of axon initial segment (AIS) because that is where Na(+) channel density is highest. To investigate the relationship between the density of Na(+) channels and the spatiotemporal pattern of AP initiation, we simultaneously recorded Na(+) flux and action currents along the proximal axonal length. We found that functional Na(+) channel density is approximately four times lower in the AP trigger zone than in the middle of the AIS, where it is highest. Computational analysis of AP initiation revealed a paradoxical mismatch between the AP threshold and Na(+) channel density, which could be explained by the lopsided capacitive load imposed on the proximal end of the AIS by the somatodendritic compartment. Favorable conditions for AP initiation are therefore achieved in the distal AIS portion, close to the edge of myelin, where the current source-load ratio is highest. Our findings suggest that cable properties play a central role in determining where the AP starts, such that small plastic changes in the local AIS Na(+) channel density could have a large influence on neuronal excitability as a whole.
authors: Department of Physiology and Cell Biology, Faculty of Health Sciences and Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev, Beer Sheva 84105, Israel.
authors: Gytis
authors: G
authors: Baranauskas
authors: Yaron
authors: Y
authors: David
authors: Ilya A
authors: IA
authors: Fleidervish
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D001369:Axons / Q000378:metabolism* / Q000648:ultrastructure; D001572:Benzofurans; D004988:Ethers, Cyclic; D005456:Fluorescent Dyes; D051379:Mice; D008959:Models, Neurological; D017966:Pyramidal Cells / Q000378:metabolism / Q000648:ultrastructure; D012964:Sodium / Q000378:metabolism*; D015222:Sodium Channels / Q000378:metabolism; D013003:Somatosensory Cortex / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D001572:Benzofurans; D004988:Ethers, Cyclic; D005456:Fluorescent Dyes; D015222:Sodium Channels; C061665:sodium-binding benzofuran isophthalate; D012964:Sodium