title: An adhesive bone marrow scaffold and bone morphogenetic-2 protein carrier for cartilage tissue engineering.
abstract: A chondroitin sulfate-bone marrow (CS-BM) adhesive hydrogel was used to localize rhBMP-2 to enhance articular cartilage tissue formation. Chondrocyte pellet culture revealed that 0.1 and 1 μg/mL of rhBMP-2 enhanced sulfated-GAG content. rhBMP-2 localization within the hydrogels was investigated, and it was found that BM, CS-NHS, and rhBMP-2 levels and time affected rhBMP-2 retention. Retention was modulated from 82 to 99% over a 3-week period for the material formulations investigated. To evaluate carrier efficacy, rhBMP-2 and bovine articular chondrocytes were encapsulated within CS-BM, and biochemical evaluation revealed significant increases in total collagen production with rhBMP-2. Histological analysis revealed more robust tissue formation and greater type-II collagen production with encapsulated rhBMP-2. Subsequently, a subcutaneous culture of hydrogels revealed increased total collagen, type-II to type-I collagen ratio, and sulfated GAG in samples carrying rhBMP-2. These findings indicate the development of a multifunctional system capable of localizing rhBMP-2 to enhance repair tissue quality.
authors: Translational Tissue Engineering Center, Wilmer Eye Institute, and Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21287, USA.
authors: Jacob A
authors: JA
authors: Simson
authors: Iossif A
authors: IA
authors: Strehin
authors: Qiaozhi
authors: Q
authors: Lu
authors: Manuel O
authors: MO
authors: Uy
authors: Jennifer H
authors: JH
authors: Elisseeff
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics* / Q000378:metabolism; D002358:Cartilage, Articular / Q000166:cytology; D002417:Cattle; D002448:Cell Adhesion; D019902:Chondrocytes / Q000737:chemistry / Q000166:cytology; D002809:Chondroitin Sulfates / Q000737:chemistry; D024042:Collagen Type I / Q000378:metabolism; D024043:Collagen Type II / Q000378:metabolism; D020100:Hydrogels / Q000737:chemistry; D051379:Mice; D008819:Mice, Nude; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D016212:Transforming Growth Factor beta / Q000235:genetics* / Q000378:metabolism; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D024042:Collagen Type I; D024043:Collagen Type II; D020100:Hydrogels; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D002809:Chondroitin Sulfates