title: Sandwich ELISA assay for the quantitation of palytoxin and its analogs in natural samples.
abstract: Palytoxins are potent marine biotoxins that have recently become endemic to the Mediterranean Sea, and are becoming more frequently associated with seafood. Due to their high toxicity, suitable methods to quantify palytoxins are needed. Thus, we developed an indirect sandwich ELISA for palytoxin and 42-hydroxy-palytoxin. An intralaboratory study demonstrated sensitivity (limit of detection, LOD = 1.1 ng/mL; limit of quantitation, LOQ = 2.2 ng/mL), accuracy (bias of 2.1%), repeatability (RSDr = 6% and 9% for intra- and interassay variability, respectively) and specificity: other common marine toxins (okadaic acid, domoic acid, saxitoxin, brevetoxin-3, and yessotoxin) do not cross-react in this assay. It performed well in three different matrices: observed LOQs were 11.0, 9.6, and 2.4 ng/mL for mussel extracts, algal net samples and seawater, respectively, with good accuracy and precision. The LOQ in seafood is 11 μg palytoxin/kg mussel meat, lower than that of the most common detection technique, LC-MS/MS.
authors: Department of Life Sciences, University of Trieste, Via L. Giorgieri 10, 34127 Trieste, Italy.
authors: S
authors: S
authors: Boscolo
authors: M
authors: M
authors: Pelin
authors: M
authors: M
authors: De Bortoli
authors: G
authors: G
authors: Fontanive
authors: A
authors: A
authors: Barreras
authors: F
authors: F
authors: Berti
authors: S
authors: S
authors: Sosa
authors: O
authors: O
authors: Chaloin
authors: A
authors: A
authors: Bianco
authors: T
authors: T
authors: Yasumoto
authors: M
authors: M
authors: Prato
authors: M
authors: M
authors: Poli
authors: A
authors: A
authors: Tubaro
mesh_terms: D000178:Acrylamides / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000915:Antibody Affinity; D003064:Cnidarian Venoms; D004784:Environmental Monitoring / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D012015:Reference Standards; D000095702:Polyether Toxins
chemical_list: D000178:Acrylamides; D003064:Cnidarian Venoms; C010272:palytoxin; D000095702:Polyether Toxins