title: In situ hybridization.
abstract: A non-radioactive in situ hybridization protocol is presented for localization of mRNA transcripts in thin tissue sections. In situ hybridization provides spatial resolution of transcript distribution at the cellular level that is not attained by Northern hybridization or PCR-based methods using organ- or tissue-derived RNA. With appropriately designed gene-specific probes, in situ hybridization can discriminate closely related gene family members. Although the procedure for tissue section preparation is tedious and lengthy, once prepared, the probe labeling, hybridization and signal detection steps can be easily scaled to handle multiple genes for comparative analysis.
authors: Warnell School of Forestry and Natural Resources, and Department of Genetics, University of Georgia, GA, USA.
authors: Chung-Jui
authors: CJ
authors: Tsai
authors: Scott A
authors: SA
authors: Harding
mesh_terms: D002021:Buffers; D005052:Eucalyptus / Q000235:genetics / Q000378:metabolism; D005404:Fixatives / Q000737:chemistry; D005557:Formaldehyde / Q000737:chemistry; D017403:In Situ Hybridization / Q000379:methods*; D008867:Microtomy; D016612:Paraffin Embedding; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D032107:Populus / Q000235:genetics / Q000378:metabolism; D015347:RNA Probes / Q000737:chemistry; D012331:RNA, Fungal / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism*; D012343:RNA, Transfer / Q000737:chemistry; D012996:Solutions; D013194:Staining and Labeling; D016707:Tissue Fixation; D020002:tert-Butyl Alcohol / Q000737:chemistry
chemical_list: D002021:Buffers; D005404:Fixatives; D010940:Plant Proteins; D011108:Polymers; D015347:RNA Probes; D012331:RNA, Fungal; D012333:RNA, Messenger; D018749:RNA, Plant; D012996:Solutions; D005557:Formaldehyde; D012343:RNA, Transfer; D020002:tert-Butyl Alcohol; C003043:paraform