title: Structural organization of the polysomes adjacent to mammalian processing bodies (P-bodies).
abstract: A finely tuned balance of translation, storage and decay of mRNAs (mRNAs) is important for the regulation of gene expression. In eukaryotic cells, this takes place in dynamic cytoplasmic RNA-protein granules termed Processing bodies (P-bodies). In this study, by using immunoelectron tomography, 3D modeling and template matching, we analyze the size and the organization of the polysomes in the vicinity of human P-bodies. Our results show the presence of several polysomes that are compatible with a translational activity around P-bodies. Therefore, movement of mRNAs between polysomes and P-bodies can take place when the two compartments are in close contact. The presence of initiation factors in the proximity of P-bodies also suggests that translation of mRNAs can resume at the periphery of these granules.
authors: Université de Rennes 1, UMR, CNRS 6290 IGDR, Translation and Folding Team, Campus de Beaulieu, Rennes Cedex, France.
authors: Nicolas
authors: N
authors: Cougot
authors: Anne-Elisabeth
authors: AE
authors: Molza
authors: Emmanuel
authors: E
authors: Giudice
authors: Annie
authors: A
authors: Cavalier
authors: Daniel
authors: D
authors: Thomas
authors: Reynald
authors: R
authors: Gillet
mesh_terms: D003594:Cytoplasmic Granules / Q000235:genetics / Q000378:metabolism*; D055032:Electron Microscope Tomography; D039561:Eukaryotic Initiation Factor-4E / Q000235:genetics / Q000378:metabolism; D039603:Eukaryotic Initiation Factor-4G / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D011132:Polyribosomes / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D014176:Protein Biosynthesis; D020871:RNA Stability; D034443:RNA Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: C491300:EIF4G2 protein, human; D039561:Eukaryotic Initiation Factor-4E; D039603:Eukaryotic Initiation Factor-4G; D012333:RNA, Messenger
keywords: P-bodies; electron tomography; initiation; mRNA; polysomes; ribosomes