title: Role of polymerase η in mitochondrial mutagenesis of Saccharomyces cerevisiae.
abstract: DNA polymerase η mostly catalyzes an error-free bypass of the most frequent UV lesions, pyrimidine dimers of the cyclobutane-type. In addition to its nuclear localization, we show here for the first time its mitochondrial localization in budding yeast. In mitochondria, this polymerase improves bypass replication fidelity opposite UV damage as shown in base pair substitution and frameshift assays. For base pair substitutions, polymerase η appears to be related in function and epistatic to DNA polymerase ζ which, however, plays the opposite role in the nucleus.
authors: Dept. of Cell Biology and Anatomy, University of North Texas Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, TX 76107, USA.
authors: Nimrat
authors: N
authors: Chatterjee
authors: Ritu
authors: R
authors: Pabla
authors: Wolfram
authors: W
authors: Siede
mesh_terms: D004260:DNA Repair; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000528:radiation effects; D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008928:Mitochondria / Q000201:enzymology* / Q000235:genetics; D016296:Mutagenesis; D011740:Pyrimidine Dimers / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics* / Q000528:radiation effects; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014466:Ultraviolet Rays; D000099270:Y-Family DNA Polymerases
chemical_list: D004272:DNA, Mitochondrial; D004259:DNA-Directed DNA Polymerase; D011740:Pyrimidine Dimers; D029701:Saccharomyces cerevisiae Proteins; C112338:Rad30 protein; D000099270:Y-Family DNA Polymerases