title: Nectin-2 and DDX3 are biomarkers for metastasis and poor prognosis of squamous cell/adenosquamous carcinomas and adenocarcinoma of gallbladder.
abstract: The clinicopathological and biological characteristics of squamous cell/adenosquamous carcinoma (SC/ASC) of gallbladder have not been well documented because it is a rare subtype of gallbladder cancer. In this study, the protein expression of Nectin-2 and DDX3 in 46 SC/ASCs and 80 adenocarcinomas was measured using immunohistochemistry. We demonstrated that positive Nectin-2 and DDX3 expression was significantly associated with large tumor size, high TNM stage, and lymph node metastasis of SC/ASC and AC. Positive Nectin-2 and DDX3 expression was significantly associated with invasion and surgical curability of AC. Univariate Kaplan-Meier analysis showed that positive Nectin-2 and DDX3 expression, degree of differentiation, tumor size, TNM stage, invasion, lymph node metastasis, and surgical curability were significantly associated with post-operative survival in both SC/ASC and AC patients. Multivariate Cox regression analysis showed that positive Nectin-2 and DDX3 expression, degree of differentiation, tumor size, TNM stage, invasion, lymph node metastasis, and no surgical curability are independent poor-prognostic factors in both SC/ASC and AC patients. Our study suggested that positive Nectin-2 and DDx3 expression is closely correlated with clinical, pathological, and biological behaviors as well as poor-prognosis of gallbladder cancer.
authors: Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University Changsha, Hunan 410011, PR China.
authors: Xiongying
authors: X
authors: Miao
authors: Zhu-Lin
authors: ZL
authors: Yang
authors: Li
authors: L
authors: Xiong
authors: Qiong
authors: Q
authors: Zou
authors: Yuan
authors: Y
authors: Yuan
authors: Jinghe
authors: J
authors: Li
authors: Lufeng
authors: L
authors: Liang
authors: Meigui
authors: M
authors: Chen
authors: Senlin
authors: S
authors: Chen
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000401:mortality / Q000556:secondary; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000401:mortality / Q000556:secondary; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D005260:Female; D005706:Gallbladder Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D000075983:Nectins; D011379:Prognosis; D016016:Proportional Hazards Models; D012307:Risk Factors
chemical_list: D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D000075983:Nectins; C119332:DDX3X protein, human; D053487:DEAD-box RNA Helicases
keywords: DDX3; Gallbladder cancer; Nectin-2; adenocarcinoma; adenosquamous carcinoma; metastasis; prognosis; squamous cell carcinoma