title: The preparation of 99mTc-DTPA-LSA and its instant lyophilized kit for hepatic receptor imaging.
abstract: Diethylenetriaminepentaacetic acid neolactosyl human serum albumin (DTPA-LSA) was prepared and labeled with technetium-99m. The labeling conditions of (99m)Tc-DTPA-LSA were optimized, and lyophilized kit was developed for instant preparing of (99m)Tc-DTPA-LSA. (99m)Tc-DTPA-LSA showed high liver uptake in normal mice (>96% ID/g at 5min after injection), and it could be blocked significantly by pre-injecting free neogalactosylalbumin (NGA). Single photon emission computed tomography (SPECT) study was performed in normal Japanese White rabbits and SPECT images with high quality were obtained at 15, 30, 60, and 120min after injection of the radiotracer. The promising biological properties of (99m)Tc-DTPA-LSA combined with the development of reliable and instant lyophilized DTPA-LSA kit afford the opportunity of hepatic receptor imaging for routine clinical assessment of hepatic function.
authors: Key Laboratory of Nuclear Analytical Techniques, Beijing Engineering Research Center of Radiographic Techniques and Equipment, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, China.
authors: Wenjiang
authors: W
authors: Yang
authors: Zuoquan
authors: Z
authors: Zhao
authors: Wei
authors: W
authors: Fang
authors: Xianzhong
authors: X
authors: Zhang
mesh_terms: D000818:Animals; D037263:Asialoglycoprotein Receptor / Q000378:metabolism; D005260:Female; D005612:Freeze Drying; D006801:Humans; D008099:Liver / Q000000981:diagnostic imaging* / Q000378:metabolism; D051379:Mice; D015609:Organotechnetium Compounds / Q000302:isolation & purification*; D011817:Rabbits; D019275:Radiopharmaceuticals / Q000302:isolation & purification*; D012709:Serum Albumin / Q000302:isolation & purification*; D013668:Technetium Tc 99m Aggregated Albumin / Q000302:isolation & purification*; D016284:Technetium Tc 99m Pentetate / Q000302:isolation & purification*; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods
chemical_list: D037263:Asialoglycoprotein Receptor; D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; D012709:Serum Albumin; D013668:Technetium Tc 99m Aggregated Albumin; C583443:technetium 99m diethylenetriaminepentaacetic acid neolactosyl human serum albumin; C057162:technetium Tc 99m DTPA HSA; D016284:Technetium Tc 99m Pentetate