title: Changes induced by Fe deficiency and Fe resupply in the root protein profile of a peach-almond hybrid rootstock.
abstract: The changes in the root extract protein profile of the Prunus hybrid GF 677 rootstock (P. dulcis × P. persica) grown in hydroponics as affected by Fe deficiency and short-term (24 h) Fe resupply have been studied by 2-dimensional gel electrophoresis-based techniques. A total of 335 spots were consistently found in the gels. Iron deficiency caused above 2-fold increases or >50% decreases in the relative abundance in 10 and 6 spots, respectively, whereas one spot was only detected in Fe-deficient plants. Iron resupply to Fe-deficient plants caused increases and decreases in relative abundance in 15 and 16 spots, respectively, and one more spot was only detected in Fe-resupplied Fe-deficient plants. Ninety-five percent of the proteins changing in relative abundance were identified using nanoliquid chromatography-tandem mass spectrometry. Defense responses against oxidative and general stress accounted for 50% of the changes in Fe-deficient roots. Also, a slight induction of the glycolysis-fermentation pathways was observed in GF 677 roots with Fe deficiency. The root protein profile of 24 h Fe-resupplied plants was similar to that of Fe-deficient plants, indicating that the deactivation of Fe-deficiency metabolic responses is slow. Taken together, our results suggest that the high tolerance of GF 677 rootstock to Fe deficiency may be related to its ability to elicit a sound defense response against both general and oxidative stress.
authors: Pomology Department, Aula Dei Experimental Station, CSIC, PO Box 13034, E-50080 Zaragoza, Spain.
authors: Jorge
authors: J
authors: Rodríguez-Celma
authors: Giuseppe
authors: G
authors: Lattanzio
authors: Sergio
authors: S
authors: Jiménez
authors: Jean-Francois
authors: JF
authors: Briat
authors: Javier
authors: J
authors: Abadía
authors: Anunciación
authors: A
authors: Abadía
authors: Yolanda
authors: Y
authors: Gogorcena
authors: Ana-Flor
authors: AF
authors: López-Millán
mesh_terms: D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D007501:Iron / Q000378:metabolism*; D000090463:Iron Deficiencies; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D040901:Proteomics; D027861:Prunus; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D010940:Plant Proteins; D007501:Iron