title: [Effects of hyaluronan tetrasaccharide on the differentiation of neuronal cells and oligodendrocyte precursor cells].
abstract: It has been reported that hyaluronan tetrasaccharide (HA4) has an ability to accelerate the healing process of spinal cord injuries and to mitigate the symptoms of experimental autoimmune encephalitis (EAE) in animal models. However, the precise mechanisms of the effect of HA4 are unclear. This study examined the in vitro effects of HA4 on the differentiation of PC12 cells and neuronal progenitor cells from mouse embryo. The effect of HA4 on the proliferation and differentiation of rat oligodendrocyte precursor cells was examined. HA4 upregulated neurite-outgrowth of cultured PC12 cells in the presence of Nerve Growth Factor (NGF), which induces neuronal differentiation in PC12. HA4 also upregulated beta III-tubulin positive cells and increased the expression of neurofilament M, two of the marker proteins of nerve differentiation. The activation was observed in an HA4 dose-dependent manner, and the effect was observed at levels as low as femtogram/L. Furthermore, the enhancement of neurite-outgrowth was not observed in the presence of hyaluronan disaccharide, hexasaccharide, 12 mer or high molecular weight hyaluronan. Upregulation of neurite-outgrowth was observed only in the presence of NGF. While HA4 accelerated neurite-outgrowth of primary mouse neural progenitor cells, it did not enhance the proliferation or differentiation of oligodendrocyte precursor cells derived from rat primary neuronal progenitor cells in culture. These results suggest that the effects of HA4 alleviating some neuronal diseases may be ascribable, at least in part, to its capacity to enhance differentiation of neurons.
authors: Section of Biochemistry, Department of Hard Tissue Engineering, Division of Bio-Matrix, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University.
authors: Hiroko
authors: H
authors: Yamanokuchi
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D006820:Hyaluronic Acid / Q000494:pharmacology*; D051379:Mice; D009474:Neurons / Q000166:cytology*; D009836:Oligodendroglia / Q000166:cytology*; D009844:Oligosaccharides; D016716:PC12 Cells; D051381:Rats; D013234:Stem Cells / Q000166:cytology; D015854:Up-Regulation
chemical_list: D009844:Oligosaccharides; D006820:Hyaluronic Acid