title: Arsenic speciation in saliva of acute promyelocytic leukemia patients undergoing arsenic trioxide treatment.
abstract: Arsenic trioxide has been successfully used as a therapeutic in the treatment of acute promyelocytic leukemia (APL). Detailed monitoring of the therapeutic arsenic and its metabolites in various accessible specimens of APL patients can contribute to improving treatment efficacy and minimizing arsenic-induced side effects. This article focuses on the determination of arsenic species in saliva samples from APL patients undergoing arsenic treatment. Saliva samples were collected from nine APL patients over three consecutive days. The patients received 10 mg arsenic trioxide each day via intravenous infusion. The saliva samples were analyzed using high-performance liquid chromatography coupled with inductively coupled plasma mass spectrometry. Monomethylarsonous acid and monomethylmonothioarsonic acid were identified along with arsenite, dimethylarsinic acid, monomethylarsonic acid, and arsenate. Arsenite was the predominant arsenic species, accounting for 71.8 % of total arsenic in the saliva. Following the arsenic infusion each day, the percentage of methylated arsenicals significantly decreased, possibly suggesting that the arsenic methylation process was saturated by the high doses immediately after the arsenic infusion. The temporal profiles of arsenic species in saliva following each arsenic infusion over 3 days have provided information on arsenic exposure, metabolism, and excretion. These results suggest that saliva can be used as an appropriate clinical biomarker for monitoring arsenic species in APL patients.
authors: Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB T6G 2G3, Canada.
authors: Baowei
authors: B
authors: Chen
authors: Fenglin
authors: F
authors: Cao
authors: Chungang
authors: C
authors: Yuan
authors: Xiufen
authors: X
authors: Lu
authors: Shengwen
authors: S
authors: Shen
authors: Jin
authors: J
authors: Zhou
authors: X Chris
authors: XC
authors: Le
mesh_terms: D000328:Adult; D000970:Antineoplastic Agents / Q000378:metabolism* / Q000493:pharmacokinetics / Q000494:pharmacology; D001149:Arsenates / Q000032:analysis; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000032:analysis / Q000378:metabolism* / Q000493:pharmacokinetics / Q000494:pharmacology; D018053:Arsenites / Q000032:analysis*; D002101:Cacodylic Acid / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004334:Drug Administration Schedule; D005260:Female; D006801:Humans; D007262:Infusions, Intravenous; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy / Q000378:metabolism*; D008297:Male; D008745:Methylation; D008875:Middle Aged; D010087:Oxides / Q000378:metabolism* / Q000493:pharmacokinetics / Q000494:pharmacology; D012463:Saliva / Q000737:chemistry*; D013054:Spectrophotometry, Atomic
chemical_list: D000970:Antineoplastic Agents; D001149:Arsenates; D001152:Arsenicals; D018053:Arsenites; D010087:Oxides; C552934:monomethylmonothioarsonic acid; D002101:Cacodylic Acid; C020300:monomethylarsonic acid; C015001:arsenite; C025657:arsenic acid; D000077237:Arsenic Trioxide