title: Early micro-rheological consequences of single fraction total body low-dose photon irradiation in mice.
abstract: Despite of the studies on widespread biological effects of irradiation, surprisingly only little number of papers can be found dealing with its in vivo hemorheological impact. Furthermore, other studies suggested that low-dose irradiation might differ from high-dose in more than linear ways. On Balb/c Jackson female adult mice hematological and hemorheological impacts of total body irradiation were investigated 1 hour following 0.002, 0.005, 0.01, 0.02, 0.05 and 0.1 Gy dose irradiation. In case of 0.01 Gy further groups were analyzed 30 minutes, 2, 4, 6, 24 and 48 h after irradiation. According to the results, it seems that the dose-dependent changes of blood micro-rheological parameters are not linear. The irradiation dose of 0.01 Gy acted as a point of 'inflexion', because by this dose we found the most expressed changes in hematological parameters, as well as in red blood cell aggregation, deformability and osmoscan data. The time-dependent changes showed progressive decrease in pH, rise in lactate concentration, further decrease in erythrocyte aggregation index and deformability, with moderate shifting of the optimal osmolarity point and modulation in membrane stability. As conclusion, low-dose total body irradiation may cause micro-rheological changes, being non-linearly correlated with the irradiation dose.
authors: Department of Radiotherapy, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary Department of Diagnostic Radiology and Oncotherapy, Semmelweis University, Budapest, Hungary.
authors: Kornelia
authors: K
authors: Szluha
authors: Department of Radiotherapy, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
authors: Kornelia
authors: K
authors: Lazanyi
authors: Department of Radiotherapy, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
authors: Andrea
authors: A
authors: Furka
authors: Department of Operative Techniques and Surgical Research, Institute of Surgery, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
authors: Ferenc
authors: F
authors: Kiss
authors: Department of Radiotherapy, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
authors: Imre
authors: I
authors: Szabo
authors: Department of Radiotherapy, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
authors: Eva
authors: E
authors: Pintye
authors: Department of Operative Techniques and Surgical Research, Institute of Surgery, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
authors: Iren
authors: I
authors: Miko
authors: Department of Operative Techniques and Surgical Research, Institute of Surgery, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
authors: Norbert
authors: N
authors: Nemeth
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis; D004307:Dose-Response Relationship, Radiation; D004903:Erythrocyte Aggregation / Q000528:radiation effects*; D004907:Erythrocyte Deformability / Q000528:radiation effects*; D004909:Erythrocyte Indices; D005260:Female; D018056:Hemorheology; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008807:Mice, Inbred BALB C; D017785:Photons; D012212:Rheology / Q000379:methods*; D013997:Time Factors; D014916:Whole-Body Irradiation
keywords: Red blood cell deformability; low-dose irradiation; mice; osmotic gradient ektacytometry; red blood cell aggregation