title: Purified collagen I oriented membrane for tendon repair: an ex vivo morphological study.
abstract: Injured tendons have limited repair ability after full-thickness lesions. Tendon regeneration properties and adverse reactions were assessed ex vivo in an experimental animal model using a new collagen I membrane. The multilamellar membrane obtained from purified equine Achilles tendon is characterized by oriented collagen I fibers and has been shown to sustain cell growth and orientation in vitro. The central third of the patellar tendon (PT) of 10 New Zealand White rabbits was sectioned and grafted with the collagen membrane; the contralateral PT was cut longitudinally (sham-operated controls). Animals were euthanized 1 or 6 months after surgery, and tendons were subjected to histological and Synchrotron Radiation-based Computed Microtomography (SRµCT) examination and 3D structure analysis. Histological and SRµCT findings showed satisfactory graft integration with native tendon. Histological examination also showed ongoing angiogenesis. Adverse side-effects (inflammation, rejection, calcification) were not observed. The multilamellar collagen I membrane can be considered as an effective tool for tendon defect repair and tendon augmentation.
authors: Department of Clinical and Molecular Sciences, School of Medicine, Università Politecnica delle Marche, Via Tronto 10/60126, Ancona, Italy. a.gigante@univpm.it
authors: Antonio
authors: A
authors: Gigante
authors: Alberto
authors: A
authors: Busilacchi
authors: Barbara
authors: B
authors: Lonzi
authors: Stefano
authors: S
authors: Cecconi
authors: Sandra
authors: S
authors: Manzotti
authors: Chiara
authors: C
authors: Renghini
authors: Alessandra
authors: A
authors: Giuliani
authors: Monica
authors: M
authors: Mattioli-Belmonte
mesh_terms: D000125:Achilles Tendon / Q000502:physiology / Q000637:transplantation; D000818:Animals; D024042:Collagen Type I / Q000302:isolation & purification / Q000502:physiology*; D004195:Disease Models, Animal; D021621:Imaging, Three-Dimensional; D008297:Male; D017847:Patellar Ligament / Q000000981:diagnostic imaging / Q000502:physiology / Q000601:surgery*; D011817:Rabbits; D019651:Plastic Surgery Procedures; D012038:Regeneration / Q000502:physiology; D013708:Tendon Injuries / Q000000981:diagnostic imaging / Q000503:physiopathology* / Q000601:surgery*; D054457:Tissue Scaffolds; D014945:Wound Healing / Q000502:physiology; D055114:X-Ray Microtomography
chemical_list: D024042:Collagen Type I