title: Isolation of a novel strain of Planomicrobium chinense from diesel contaminated soil of tropical environment.
abstract: A novel bacterial strain (B6) degrading high concentration of diesel oil [up to 2.5% (v/v)] was isolated from a site contaminated with petroleum hydrocarbons in the state of Chhattisgarh, India. The strain demonstrated efficient degradation for diesel oil range alkanes (C14 to C36 i.e., mostly linear chain alkanes). It was identified to be 99% similar to Planomicrobium chinense on the basis of partial 16S rRNA gene sequencing and biochemical characteristics. The efficiency of degradation was optimized at pH 7.2 and temperature at 32 °C. GC analysis demonstrated complete mineralization of higher chain alkanes into lower chain alkanes within 96 h. The organism also displayed surface tension reduction by producing stable emulsification on the onset of stationary phase. A multidimensional characteristics of the strain to grow at a high temperature range, resistance to various heavy metals as well as tolerance to moderate concentration of NaCl makes it suitable for bioremediation of soil contaminated with diesel oil in tropical environment.
authors: Department of Biotechnology, Guru Ghasidas Vishwavidyalaya Central University, Bilaspur, India.
authors: Reena
authors: R
authors: Das
authors: Bhupendra N
authors: BN
authors: Tiwary
mesh_terms: D015373:Bacterial Typing Techniques; D001673:Biodegradation, Environmental; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005742:Gasoline; D006838:Hydrocarbons / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D058641:Planococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013696:Temperature; D014329:Tropical Climate
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005742:Gasoline; D006838:Hydrocarbons; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants
keywords: Bioremediation; Biosurfactant; Diesel oil; Hydrocarbons; Petroleum; Planomicrobium chinense