title: Development of an ERIC sequence typing scheme for Laribacter hongkongensis, an emerging pathogen associated with community-acquired gastroenteritis and travellers' diarrhoea.
abstract: Laribacter hongkongensis is a potential emerging pathogen, associated with community-acquired diarrhoea. For epidemiological purposes, different molecular typing methods, such as pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing, have been developed for this pathogen. However, these methods require specialized equipment and costly reagents. More importantly, they are labour-intensive and time-consuming, which is not really suitable for foodborne disease outbreak investigations. In this study, we developed a rapid and reliable method using 22-mer primers specific for the enterobacterial repetitive intergenic consensus sequence (ERIC). PFGE was used for comparison, to evaluate this method. A total of 81 isolates of L. hongkongensis were examined: 79 isolates recovered from food of diverse origins and two strains derived from patients with L. hongkongensis-associated infection. Typing patterns and clustering analysis indicated that the 81 L. hongkongensis isolates were grouped into 21 and 13 genotypes by ERIC-PCR and PFGE, respectively. ERIC-PCR was found as reproducible as PFGE. A high percentage (70.4%) of isolates yielded distinguishable ERIC-PCR patterns, which were concordant with the results from PFGE. These results suggest that ERIC-PCR is valuable for use in the epidemiological investigation of L. hongkongensis.
authors: Key Laboratory of Prevention and Control of Emerging Infectious Diseases of Guangdong Higher Education Institutes, Southern Medical University, Guangzhou, Guangdong Province 510515, China.|Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
authors: Jia-Li
authors: JL
authors: Feng
authors: Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
authors: Jin-Yan
authors: JY
authors: Lin
authors: College of Light Industry and Food Science, South China University of Technology Guangzhou, 510000, China.
authors: Xiao-Bing
authors: XB
authors: Jiang
authors: Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
authors: Ou
authors: O
authors: Zhang
authors: Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
authors: Jiang-Feng
authors: JF
authors: Zhu
authors: Key Laboratory of Prevention and Control of Emerging Infectious Diseases of Guangdong Higher Education Institutes, Southern Medical University, Guangzhou, Guangdong Province 510515, China.|Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
authors: Jing
authors: J
authors: Hu
authors: College of Light Industry and Food Science, South China University of Technology Guangzhou, 510000, China.
authors: Lei
authors: L
authors: Shi
authors: Key Laboratory of Prevention and Control of Emerging Infectious Diseases of Guangdong Higher Education Institutes, Southern Medical University, Guangzhou, Guangdong Province 510515, China.|Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.
authors: Qing
authors: Q
authors: Chen
mesh_terms: D000818:Animals; D002347:Carps / Q000382:microbiology; D021821:Communicable Diseases, Emerging; D017714:Community-Acquired Infections / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D003967:Diarrhea / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D005516:Food Microbiology; D005759:Gastroenteritis / Q000382:microbiology*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D009346:Neisseriaceae / Q000145:classification / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011898:Ranidae / Q000382:microbiology; D014195:Travel
chemical_list: D004269:DNA, Bacterial