title: [Intraspecific variation analysis of Conidiobolus coronatus using RAPD and ITS sequencing].
abstract: BACKGROUND
  The fungus Conidiobolus coronatus (C. coronatus) has an extensive distribution of habitats and hosts. It is found saprophytically, and attacks insects and mammals, including humans. Although there are few reports on humans, and they are restricted to tropical areas. The aim of this work was to determine whether genetic variation exists between C. coronatus isolates coming from human lesions and other sources.
  
  
  METHODS
  A total of 11C. coronatus isolates obtained from soil, insects and humans were analyzed with the random amplification of polymorphic ADN (RAPD) and internal transcribed spacer (ITS) techniques; the maximum parsimony, neighbour-joining and minimum evolution methods were used for the ITS analysis.
  
  
  RESULTS
  The analysis of the C. coronatus polymorphisms showed high intra-species variation levels between the evaluated isolates. The isolates coming from human lesions showed the greatest genetic divergence compared with the remaining isolates. The greatest genetic distance between isolate groups was found between those coming from humans and those taken from the insect Lycoriella ingenua.
  
  
  CONCLUSIONS
  This is the very first work evaluating and demonstrating that within species variation exists at molecular level in C. coronatus, and is related to the source where the isolates were taken from.
authors: Instituto de Fitosanidad, Colegio de Postgraduados, Campus Montecillo, Texcoco, México. caroperez@colpos.mx
authors: Carolina
authors: C
authors: Pérez-Martínez
authors: Reyna I
authors: RI
authors: Rojas-Martínez
authors: Raquel
authors: R
authors: Alatorre-Rosas
authors: Francisco
authors: F
authors: Hernández-Rosas
authors: Gabriel
authors: G
authors: Otero-Colina
mesh_terms: D020092:Conidiobolus / Q000145:classification* / Q000235:genetics*; D004271:DNA, Fungal / Q000032:analysis; D006801:Humans; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D004271:DNA, Fungal