title: Next-generation sequencing technologies and their impact on microbial genomics.
abstract: Next-generation sequencing technologies have had a dramatic impact in the field of genomic research through the provision of a low cost, high-throughput alternative to traditional capillary sequencers. These new sequencing methods have surpassed their original scope and now provide a range of utility-based applications, which allow for a more comprehensive analysis of the structure and content of microbial genomes than was previously possible. With the commercialization of a third generation of sequencing technologies imminent, we discuss the applications of current next-generation sequencing methods and explore their impact on and contribution to microbial genome research.
authors: Department of Microbiology, University College Cork, Cork, Ireland. pwotoole@ucc.ie.
authors: Brian M
authors: BM
authors: Forde
authors: Paul W
authors: PW
authors: O'Toole
mesh_terms: D001419:Bacteria / Q000235:genetics*; D016680:Genome, Bacterial / Q000235:genetics; D023281:Genomics / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D056186:Metagenomics
keywords: NGS; RNA-seq; genome sequencing; metagenomics; prokaryotes; resequencing