title: Disseminated Mycobacterium tuberculosis infection in a dog.
abstract: An uncommon disseminated Mycobacterium tuberculosis infection is described in a 12-year-old female dog presenting with fever, dyspnea, cough, weight loss, lymphadenopathy, melena, epistaxis, and emesis. The dog had a history of close contact with its owner, who died of pulmonary tuberculosis. Radiographic examination revealed diffuse radio-opaque images in both lung lobes, diffuse visible masses in abdominal organs, and hilar and mesenteric lymphadenopathy. Bronchial washing samples and feces were negative for acid-fast organisms. Polymerase chain reaction (PCR)-based species identification of bronchial washing samples, feces, and urine revealed M. tuberculosis using PCR-restriction enzyme pattern analysis-PRA. Because of public health concerns, which were worsened by the physical condition of the dog, euthanasia of the animal was recommended. Rough and tough colonies suggestive of M. tuberculosis were observed after microbiological culture of lung, liver, spleen, heart, and lymph node fragments in Löwenstein-Jensen and Stonebrink media. The PRA analysis enabled diagnosis of M. tuberculosis strains isolated from organs.
authors: Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science, UNESP - Univ. Estadual Paulista, Botucatu, State of Sao Paulo, Brazil. aninhavitty@uol.com.br
authors: Anna Paula Vitirito
authors: AP
authors: Martinho
authors: Marília Masello Junqueira
authors: MM
authors: Franco
authors: Márcio Garcia
authors: MG
authors: Ribeiro
authors: Isabella Belletti Mutt
authors: IB
authors: Perrotti
authors: Simone Henriques
authors: SH
authors: Mangia
authors: Jane
authors: J
authors: Megid
authors: Luiz Carlos
authors: LC
authors: Vulcano
authors: Gustavo Henrique Batista
authors: GH
authors: Lara
authors: Adolfo Carlos Barreto
authors: AC
authors: Santos
authors: Clarice Queico Fujimura
authors: CQ
authors: Leite
authors: Osimar
authors: O
authors: de Carvalho Sanches
authors: Antonio Carlos
authors: AC
authors: Paes
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D004283:Dog Diseases / Q000382:microbiology* / Q000473:pathology; D004285:Dogs; D005243:Feces / Q000382:microbiology; D005260:Female; D008168:Lung / Q000382:microbiology / Q000473:pathology; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D014376:Tuberculosis / Q000382:microbiology / Q000473:pathology / Q000652:urine / Q000662:veterinary*