title: Oxidative modifications of cerebral transthyretin are associated with multiple sclerosis.
abstract: Transthyretin (TTR) is a homotetrameric protein of the CNS that plays a role of as the major thyroxine (T4) carrier from blood to cerebrospinal fluid (CSF). T4 physiologically helps oligodendrocyte precursor cells to turn into myelinating oligodendrocytes, enhancing remyelination after myelin sheet damage. We investigated post-translational oxidative modifications of serum and CSF TTR in multiple sclerosis subjects, highlighting high levels of S-sulfhydration and S-sulfonation of cysteine in position ten only in the cerebral TTR, which correlate with an anomalous TTR protein folding as well as with disease duration. Moreover, we found low levels of free T4 in CSF of multiple sclerosis patients, suggestive of a potential role of these modifications in T4 transport into the brain.
authors: Research Centre on Aging (Ce.S.I), University G. d'Annunzio of Chieti-Pescara, Chieti, Italy. dpieragostino@unich.it
authors: Damiana
authors: D
authors: Pieragostino
authors: Piero
authors: P
authors: Del Boccio
authors: Maria
authors: M
authors: Di Ioia
authors: Luisa
authors: L
authors: Pieroni
authors: Viviana
authors: V
authors: Greco
authors: Giovanna
authors: G
authors: De Luca
authors: Simona
authors: S
authors: D'Aguanno
authors: Claudia
authors: C
authors: Rossi
authors: Diego
authors: D
authors: Franciotta
authors: Diego
authors: D
authors: Centonze
authors: Paolo
authors: P
authors: Sacchetta
authors: Carmine
authors: C
authors: Di Ilio
authors: Alessandra
authors: A
authors: Lugaresi
authors: Andrea
authors: A
authors: Urbani
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid*; D010084:Oxidation-Reduction; D011228:Prealbumin / Q000134:cerebrospinal fluid* / Q000737:chemistry / Q000302:isolation & purification; D020033:Protein Isoforms / Q000134:cerebrospinal fluid / Q000737:chemistry / Q000302:isolation & purification; D011499:Protein Processing, Post-Translational; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013974:Thyroxine / Q000134:cerebrospinal fluid
chemical_list: D011228:Prealbumin; D020033:Protein Isoforms; D013974:Thyroxine