title: Layer by layer assembly of biotinylated protein networks for signal amplification.
abstract: Described herein is a unique and inexpensive method that outperforms commercial methods that amplify the streptavidin-biotin recognition event. Amplification induced by streptavidin and biotinylated protein causes the formation of a large detectable polymer. This approach enjoys a 100-fold decrease in detection limit in comparison with the commercial methods.
authors: Department of Applied Chemistry, National University of Kaohsiung, 700 Kaohsiung University Road, Kaohsiung 811, Taiwan.
authors: Yu W
authors: YW
authors: Chu
authors: Bo Y
authors: BY
authors: Wang
authors: Huei-Shian
authors: HS
authors: Lin
authors: Tai-Yen
authors: TY
authors: Lin
authors: Yuan-Jen
authors: YJ
authors: Hung
authors: David A
authors: DA
authors: Engebretson
authors: Wonjae
authors: W
authors: Lee
authors: James R
authors: JR
authors: Carey
mesh_terms: D000818:Animals; D001360:Avidin / Q000737:chemistry / Q000378:metabolism; D001710:Biotin / Q000737:chemistry* / Q000378:metabolism; D019921:Biotinylation; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D011506:Proteins; D001360:Avidin; D012710:Serum Albumin, Bovine; D001710:Biotin; D019809:Streptavidin