title: Antinociceptive and anti-inflammatory effects of Aloe saponaria Haw on thermal injury in rats.
abstract: BACKGROUND
  In Brazil, the plant Aloe saponaria Haw, popularly known as "babosa pintadinha", has been empirically used for its potential effect on thermal injury. Because there are no scientific data confirming its popular use, the aim of the present study was to investigate the effects of Aloe saponaria on nociceptive and inflammatory parameters in a rat model of thermal injury.
  
  
  METHODS
  Adult male Wistar rats were subjected to a thermal injury or sham procedure (immersion in water at 70 or 37°C, respectively, for 5 or 8s). Burned animals were topically treated with vehicle (base cream), sulfadiazine 1% (positive control) or Aloe saponaria cream (0.3%-30%) once a day for 2 or 6 days. Each day, 30min before the treatment, we measured nociceptive (static and dynamic mechanical allodynia, thermal allodynia and spontaneous pain) and inflammatory (paw edema) parameters. Moreover, enzymatic indicators of leukocyte infiltration into burned tissue were also determined 2 or 6 days after the thermal injury.
  
  
  RESULTS
  The thermal injury (fist and second-degree) procedure, but not the sham procedure, induced nociception and inflammation from 1 to 6 days after the injury. The topical treatment with Aloe saponaria cream (10%) reduced nociceptive behaviors from day 1 to 6 (peak at day 2), edema at days 5 and 6 (peak at day 6) and myeloperoxidase, N-acetyl-glucosaminidase and eosinoperoxidase activities at day 6. The antinociceptive and anti-inflammatory effects of Aloe saponaria were obtained with doses of 3%-30%, with maximal inhibition obtained with a dose of 10% (reductions of 39±9%, 41±9%, 31±7%, 83±7% and 23±2% for static and dynamic mechanical allodynia, thermal allodynia, spontaneous pain and paw edema, respectively).
  
  
  CONCLUSIONS
  Our results demonstrate that topically applied Aloe saponaria presented antinociceptive and anti-inflammatory effects in rats subjected to a thermal injury, which supports its traditional use for burn injuries.
authors: Programa de Pós Graduação em Ciências Biológicas: Bioquímica Toxicológica, Universidade Federal de Santa Maria, Camobi, Santa Maria 97109-900, RS, Brazil.
authors: Mariane Arnoldi
authors: MA
authors: Silva
authors: Gabriela
authors: G
authors: Trevisan
authors: Jonatas Zeni
authors: JZ
authors: Klafke
authors: Mateus Fortes
authors: MF
authors: Rossato
authors: Cristiani Isabel Bandero
authors: CI
authors: Walker
authors: Sara Marchesan
authors: SM
authors: Oliveira
authors: Cássia Regina
authors: CR
authors: Silva
authors: Aline Augusti
authors: AA
authors: Boligon
authors: Fernanda Cramer
authors: FC
authors: Flores
authors: Cristiane
authors: C
authors: de Bona Silva
authors: Margareth Linde
authors: ML
authors: Athayde
authors: Juliano
authors: J
authors: Ferreira
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D000504:Aloe; D000700:Analgesics / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D001522:Behavior, Animal / Q000187:drug effects; D002056:Burns / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D004487:Edema / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology; D047209:Eosinophil Peroxidase / Q000378:metabolism; D005419:Flavonoids / Q000302:isolation & purification; D006358:Hot Temperature; D006930:Hyperalgesia / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D010636:Phenols / Q000302:isolation & purification; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D047209:Eosinophil Peroxidase; D009195:Peroxidase; D000118:Acetylglucosaminidase