title: Label-free electrochemical detection of an Entamoeba histolytica antigen using cell-free yeast-scFv probes.
abstract: Inexpensive, simple and quick detection of pathogen antigens in human samples is a key global health objective. Limiting factors include the cost and complexity of diagnostic tests that utilize antibody probes. Herein, we present a method for label-free electrochemical detection of a protein from the enteric pathogen Entamoeba histolytica using cell-free yeast-embedded antibody-like fragments (yeast-scFv) as novel affinity reagents.
authors: Centre for Biomarker Research and Development, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.
authors: Yadveer S
authors: YS
authors: Grewal
authors: Muhammad J A
authors: MJ
authors: Shiddiky
authors: Sean A
authors: SA
authors: Gray
authors: Kris M
authors: KM
authors: Weigel
authors: Gerard A
authors: GA
authors: Cangelosi
authors: Matt
authors: M
authors: Trau
mesh_terms: D000953:Antigens, Protozoan / Q000032:analysis* / Q000276:immunology; D055664:Electrochemical Techniques; D004566:Electrodes; D004579:Electron Transport; D004748:Entamoeba histolytica / Q000378:metabolism*; D005292:Ferricyanides / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006801:Humans; D007118:Immunoassay; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D057127:Single-Chain Antibodies / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: D000953:Antigens, Protozoan; D005292:Ferricyanides; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies; C007931:hexacyanoferrate III; D006046:Gold