title: Determination of paroxetine in serum treated with simple pretreatment by pre-column high-performance liquid chromatography using 4-(5,6-dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl chloride as a fluorescent labeling reagent.
abstract: The therapeutic drug monitoring of paroxetine could be used to optimize the pharmacological treatment of depressed patients. A simple and sensitive high-performance liquid chromatography procedure was developed for the determination of paroxetine in serum. After simple pretreatment of serum (50 μL) with acetonitrile and o-phthalaldehyde, paroxetine was derivatized with 4-(5,6-dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl chloride at 70°C for 20 min in borate buffer (0.1 mol/L, pH 8.0) to produce a fluorescent product. The derivative was separated on a reversed-phase column at 40°C for stepwise elution with (A) acetic acid (10 mmol/L) and (B) acetonitrile. The flow rate was 1.0 mL/min. The fluorescence intensity was monitored at excitation and emission wavelengths of 320 and 400 nm, respectively. The within-day and day-to-day relative standard deviations were 3.0-3.4 and 2.7-8.3%, respectively. The detection limit of paroxetine was 8.3 fmol at a signal-to-noise ratio of 3. As the proposed method that only requires a small quantity of serum (50 μL) is simple, sensitive and reproducible, it would be useful for clinical and biochemical research as well as drug monitoring.
authors: Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Fukuyama, Hiroshima, 729-0292, Japan.
authors: Hirofumi
authors: H
authors: Inoue
authors: Takuya
authors: T
authors: Harada
authors: Seiji
authors: S
authors: Eto
authors: Ken
authors: K
authors: Nakashima
authors: Takuya
authors: T
authors: Ibusuki
authors: Keiko
authors: K
authors: Kosha
authors: Yuuko
authors: Y
authors: Date
authors: Emiko
authors: E
authors: Sanematsu
authors: Yoshitake
authors: Y
authors: Shinohara
authors: Kojiro
authors: K
authors: Takahashi
authors: Reiji
authors: R
authors: Yoshimura
authors: Jun
authors: J
authors: Nakamura
authors: Eijiro
authors: E
authors: Kojima
authors: Yasuto
authors: Y
authors: Tsuruta
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008297:Male; D008875:Middle Aged; D017374:Paroxetine / Q000097:blood* / Q000737:chemistry; D010797:Phthalimides / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: C117624:4-(5,6-dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl chloride; D005456:Fluorescent Dyes; D010797:Phthalimides; D017374:Paroxetine