title: Tissue-specific control of brain-enriched miR-7 biogenesis.
abstract: MicroRNA (miRNA) biogenesis is a highly regulated process in eukaryotic cells. Several mature miRNAs exhibit a tissue-specific pattern of expression without an apparent tissue-specific pattern for their corresponding primary transcripts. This discrepancy is suggestive of post-transcriptional regulation of miRNA abundance. Here, we demonstrate that the brain-enriched expression of miR-7, which is processed from the ubiquitous hnRNP K pre-mRNA transcript, is achieved by inhibition of its biogenesis in nonbrain cells in both human and mouse systems. Using stable isotope labeling by amino acids in cell culture (SILAC) mass spectrometry combined with RNase-assisted RNA pull-down, we identified Musashi homolog 2 (MSI2) and Hu antigen R (HuR) proteins as inhibitors of miR-7 processing in nonneural cells. This is achieved through HuR-mediated binding of MSI2 to the conserved terminal loop of pri-miR-7. Footprinting and electrophoretic gel mobility shift analysis (EMSA) provide further evidence for a direct interaction between pri-miR-7-1 and the HuR/MSI2 complex, resulting in stabilization of the pri-miR-7-1 structure. We also confirmed the physiological relevance of this inhibitory mechanism in a neuronal differentiation system using human SH-SY5Y cells. Finally, we show elevated levels of miR-7 in selected tissues from MSI2 knockout (KO) mice without apparent changes in the abundance of the pri-miR-7 transcript. Altogether, our data provide the first insight into the regulation of brain-enriched miRNA processing by defined tissue-specific factors.
authors: Wellcome Trust Centre for Cell Biology, University of Edinburgh, United Kingdom.
authors: Nila Roy
authors: NR
authors: Choudhury
authors: Flavia
authors: F
authors: de Lima Alves
authors: Luisa
authors: L
authors: de Andrés-Aguayo
authors: Thomas
authors: T
authors: Graf
authors: Javier F
authors: JF
authors: Cáceres
authors: Juri
authors: J
authors: Rappsilber
authors: Gracjan
authors: G
authors: Michlewski
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D005786:Gene Expression Regulation; D051379:Mice; D018345:Mice, Knockout; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D011485:Protein Binding; D055550:Protein Stability; D016601:RNA-Binding Proteins / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; C549242:MIRN7-1 microRNA, human; C549243:MIRN7 microRNA, mouse; C499059:Msi2h protein, mouse