title: GATA4 and DcR1 methylation in glioblastomas.
abstract: BACKGROUND
  Epigenetic silencing of tumor suppressor genes plays important role in gliomagenesis. Recently, GATA4 and DcR1 were suggested to be a tumor suppressor genes involved in tumorigenesis in various types of human cancers. However, up to now the methylation frequency of GATA4 and DcR1 genes has not been determined in glioblastoma. In this study, we investigated methylation of GATA4 and DcR1 promoters and their association with patient prognosis in glioblastoma.
  
  
  METHODS
  Methylation status of GATA4 and DcR1 promoters was investigated by methylation specific PCR in 99 glioblastoma patients. Statistical analyses were conducted to investigate the association between clinical variables and overall survival time.
  
  
  RESULTS
  GATA4 and DcR1 were aberrantly methylated in 23.2% and 27.6% of glioblastoma tumors, but not in normal brain. GATA4 promoter hypermethylation showed significant association with patients age (p = 0.027). Relationship between genes promoter methylation and glioblastoma patient survival was not determined.
  
  
  CONCLUSIONS
  The present work demonstrated that GATA4 and DcR1 promoter hypermethylation is tumor specific event in glioblastoma but they promoter methylation cannot be considered as a prognostic marker of glioblastoma survival.
  The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1381170351801852.
authors: Laboratory of Neurooncology and Genetics, Neuroscience Institute, Kaunas Academy of Medicine, Lithuanian University of Health Sciences, Eiveniu str 4, Kaunas, LT, Lithuania. p.grigaite@gmail.com.
authors: Paulina
authors: P
authors: Vaitkienė
authors: Daina
authors: D
authors: Skiriutė
authors: Kęstutis
authors: K
authors: Skauminas
authors: Arimantas
authors: A
authors: Tamašauskas
mesh_terms: D000367:Age Factors; D001932:Brain Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D016022:Case-Control Studies; D019175:DNA Methylation; D005260:Female; D050991:GATA4 Transcription Factor / Q000235:genetics*; D058851:GPI-Linked Proteins / Q000235:genetics; D016147:Genes, Tumor Suppressor; D020022:Genetic Predisposition to Disease; D005909:Glioblastoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011379:Prognosis; D011401:Promoter Regions, Genetic; D053320:Receptors, Tumor Necrosis Factor, Member 10c; D012307:Risk Factors; D013997:Time Factors; D053319:Tumor Necrosis Factor Decoy Receptors / Q000235:genetics*
chemical_list: D050991:GATA4 Transcription Factor; C484170:GATA4 protein, human; D058851:GPI-Linked Proteins; D053320:Receptors, Tumor Necrosis Factor, Member 10c; C108661:TNFRSF10C protein, human; D053319:Tumor Necrosis Factor Decoy Receptors