title: Characterization of various blood and graft sources: a prospective series.
abstract: BACKGROUND
  Studies comparing cell components of blood and graft sources are very scarce. We present here a thorough study examining the cellular content of various sources of blood and cell therapy products.
  
  
  METHODS
  We have prospectively compared by fluorescence-activated cell sorting analyses the cellular composition of three blood sources on the one hand--peripheral blood (PB; n = 10) versus granulocyte-colony-stimulating factor (G-CSF)-mobilized PB (GCSF-PB, n = 10) versus cord blood (CB, n = 10)--and of three graft sources on the other hand--unmanipulated bone marrow (uBM, n = 5) versus leukapheresis product (LP, n = 10) versus thawed CB graft (n = 7).
  
  
  RESULTS
  All median absolute numbers of cell subsets were found significantly higher in GCSF-PB and LP, except for monocytoid dendritic cells (mDCs) in CB and uBM. The most impressive results were the median quantities of memory T and B lymphocytes but also of plasmacytoid DCs (pDCs) contained in LP compared to thawed CB graft, with ratios of 375, 318, and 247, respectively. The proportions of naive and CD4+/CD8- T cells, transitional B cells, and CD5+ and naive B lymphocytes were found significantly higher in CB samples while the proportions of mDCs and pDCs were found significantly lower.
  
  
  CONCLUSIONS
  Our study shows strong differences in terms of quantitative and qualitative cellular composition between several blood or graft sources, possibly explaining the differences observed in terms of outcomes after transplant.
authors: Service d'Hématologie Clinique, CHU Hôtel Dieu, Université de Nantes, Centre d'Investigation Clinique en Cancerologie (CI2C) and INSERM U892; Service d'Hematologie/Biologie, Service de Gynecologie/Obstetrique; Cellule de Promotion à la Recherche Clinique, CHU de Nantes; Service de Virologie, CHU de Nantes, and UPRES-EA4271, PRES L'UNAM, Nantes University; Unité d'Ingénierie Cellulaire, EFS Pays de la Loire, Nantes, France.
authors: Patrice
authors: P
authors: Chevallier
authors: Nelly
authors: N
authors: Robillard
authors: Marina
authors: M
authors: Illiaquer
authors: Julie
authors: J
authors: Esbelin
authors: Mohamad
authors: M
authors: Mohty
authors: Celine
authors: C
authors: Bodin-Bressollette
authors: Thierry
authors: T
authors: Guillaume
authors: Veronique
authors: V
authors: Stocco
authors: Fabienne
authors: F
authors: Auffray
authors: Sophie
authors: S
authors: Derenne
authors: Lucie
authors: L
authors: Planche
authors: Marie-Christine
authors: MC
authors: Bene
authors: Berthe-Marie
authors: BM
authors: Imbert-Marcille
mesh_terms: D016026:Bone Marrow Transplantation / Q000379:methods; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D006086:Graft vs Host Disease / Q000517:prevention & control; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods; D006801:Humans; D011446:Prospective Studies; D033581:Stem Cell Transplantation / Q000379:methods*; D014184:Transplantation, Homologous / Q000379:methods*
chemical_list: D016179:Granulocyte Colony-Stimulating Factor