title: Viper venom induced inflammation with Montivipera xanthina (Gray, 1849) and the anti-snake venom activities of Artemisia absinthium L. in rat.
abstract: The present study was conducted to explore the characterization of Montivipera xanthina crude venom partially by in vitro and in vivo and the anti-snake venom activities of Artemisia absinthium L. in comparison with carrageenan-induced acute inflammation model in rats. The LD50 value was estimated as 8.78 mg/kg within 24 h by different venom doses administrated intraperitoneally in mice. The IC50 value was 0.43 ± 0.18 μg/ml after 48 h treatment while the calculated value was 0.73 ± 0.10 μg/ml for the culture media totally refreshed after 2 h treatment with venom. Wistar rats were treated intraperitoneally with A. absinthium extract, 30 min before venom or carrageenan was injected subplantarly into the left hind paw. Intraperitoneal administration of 25 and 50 mg/kg extract was inhibited venom induced paw swelling at 0.5, 1, 2 and 3 h (p < 0.05) while 12.5, 25 and 50 mg/kg extract treatment was inhibited carrageenan-induced paw swelling at 2, 3, 4 and 5 h (p < 0.05). In conclusion, the in vivo toxicity and inflammatory actions and in vitro cytotoxic actions of crude M. xanthina venom were performed as a first report and inhibition of venom-induced inflammation by methanolic extract of A. absinthium was described.
authors: Ege University, Faculty of Engineering, Department of Bioengineering, 35100 Bornova, Izmir, Turkey. analbantsoy@gmail.com
authors: Ayse
authors: A
authors: Nalbantsoy
authors: Sura Baykan
authors: SB
authors: Erel
authors: Cinel
authors: C
authors: Köksal
authors: Bayram
authors: B
authors: Göçmen
authors: Mehmet Zülfü
authors: MZ
authors: Yıldız
authors: Nefise Ülkü
authors: NÜ
authors: Karabay Yavaşoğlu
mesh_terms: D000818:Animals; D000997:Antivenins / Q000302:isolation & purification / Q000627:therapeutic use*; D031171:Artemisia absinthium / Q000737:chemistry*; D002351:Carrageenan; D007249:Inflammation / Q000139:chemically induced* / Q000188:drug therapy; D007928:Lethal Dose 50; D051379:Mice; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D014757:Viper Venoms / Q000633:toxicity*; D017819:Viperidae
chemical_list: D000997:Antivenins; D010936:Plant Extracts; D014757:Viper Venoms; D002351:Carrageenan