title: Superoxide dismutases from larvae of the camel tick Hyalomma dromedarii.
abstract: Three superoxide dismutases (EC 1.15.1.1) (TLSOD1, TLSOD2 and TLSOD3) were purified from larvae of the camel tick Hyalomma dromedarii by ammonium sulfate precipitation, ion exchange and gel filtration columns. SDS-PAGE revealed that the subunit molecular masses of the SODs are 40±2 kDa, 67±1.5 kDa and 45±2.6 kDa for TLSOD1, TLSOD2 and TLSOD3, respectively. TLSOD1 and TLSOD2 are monomeric proteins, while TLSOD3 isoenzyme exhibits dimeric structure with native molecular mass of 90 kDa. The pI values are estimated at pH 8.0, pH 7.2 and pH 6.6 for the three SODs which displayed pH optima at 7.6, 8.0 and 7.8, respectively. CuCl(2) and ZnCl(2) increase the activity of TLSOD2 and TLSOD3, while MnCl(2) increases the activity of TLSOD1. KCN inhibits the activity of TLSOD2 and TLSOD3, while a remarkable resistance of TLSOD1 isoenzyme was detected. TLSOD1 is suggested to be a manganese containing isoenzyme while TLSOD2 and TLSOD3 are suggested to be copper/zinc-containing isoenzymes. These results indicate the presence of three different forms of SODs in the larval stage of camel tick. This finding will contribute to our understanding of the physiology of these ectoparasites and the development of non-traditional methods to control them.
authors: Molecular Biology Department, National Research Centre, El-Tahrir st., Dokki, Cairo, Egypt. ibrahimm70@hotmail.com
authors: Mahmoud A
authors: MA
authors: Ibrahim
authors: Mona M
authors: MM
authors: Mohamed
authors: Abdel-Hady M
authors: AH
authors: Ghazy
authors: Hassan M M
authors: HM
authors: Masoud
mesh_terms: D000818:Animals; D002162:Camelus / Q000469:parasitology*; D002413:Cations, Divalent / Q000494:pharmacology; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D047108:Embryonic Development / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D007814:Larva / Q000187:drug effects / Q000201:enzymology; D008970:Molecular Weight; D013482:Superoxide Dismutase / Q000302:isolation & purification* / Q000378:metabolism; D013987:Ticks / Q000187:drug effects / Q000196:embryology / Q000201:enzymology*
chemical_list: D002413:Cations, Divalent; D004791:Enzyme Inhibitors; D007527:Isoenzymes; D013482:Superoxide Dismutase