title: Enhanced sonographic imaging to diagnose lymph node metastasis: importance of blood vessel volume and density.
abstract: Lymph node size is an important variable in ultrasound diagnosis of lymph node metastasis. However, the size criterion often leads to oversight of tumor-positive lymph nodes within the range of "normal" size, such that more accurate diagnostic criteria for lymph node metastasis are required. In this study, we show how diagnosis of lymph node metastasis can be improved by evaluating changes in blood vessel volume and density using a novel contrast-enhanced high-frequency ultrasound (CE-HFUS) system with Sonazoid. An MRL/MpJ-lpr/lpr (MRL/lpr) mouse model of lymph node metastasis was used in which lymph nodes are similar in size to humans. Metastasis via lymphatic vessels to proper axillary lymph nodes (proper ALN) was induced by injection of tumor cells into the subiliac lymph nodes. Within 21 days of injection, significant increases in blood vessel volume and density, but no increases in the size of the proper ALNs, were observed. The increase in blood vessel density was confirmed with immunohistochemical analysis and was positively related to tumor cell proliferation as measured using bioluminescence imaging. Together, our results showed that alterations in blood vessel volume and density precede alterations in lymph node size in the early stages of lymph node metastasis. Detection of these changes by ultrasonography may offer new criteria for early diagnosis of lymph node metastasis.
authors: Department of Biomedical Engineering, Graduate School of Biomedical Engineering, Tohoku University, Sendai, Miyagi, Japan.
authors: Li
authors: L
authors: Li
authors: Shiro
authors: S
authors: Mori
authors: Mizuho
authors: M
authors: Kodama
authors: Maya
authors: M
authors: Sakamoto
authors: Shoki
authors: S
authors: Takahashi
authors: Tetsuya
authors: T
authors: Kodama
mesh_terms: D000818:Animals; D001365:Axilla; D001808:Blood Vessels / Q000000981:diagnostic imaging / Q000473:pathology*; D001810:Blood Volume; D005260:Female; D051677:Histiocytoma, Malignant Fibrous / Q000000981:diagnostic imaging / Q000473:pathology*; D007124:Immunoenzyme Techniques; D008198:Lymph Nodes / Q000000981:diagnostic imaging / Q000473:pathology*; D008206:Lymphatic Diseases / Q000000981:diagnostic imaging / Q000473:pathology*; D008207:Lymphatic Metastasis; D051379:Mice; D021701:Sentinel Lymph Node Biopsy; D014463:Ultrasonography