title: Developmental potential of pig embryos reconstructed by use of sow versus pre-pubertal gilt oocytes after somatic cell nuclear transfer.
abstract: In this study, the developmental ability of cloned embryos using gilt versus sow oocytes was evaluated under the hypothesis that the efficiency of nuclear transfer using gilt oocytes was lower than that of sow oocytes, but that it could be optimized. Five experiments were performed with routine production of cloned embryos with sow oocytes serving as the control. Results showed that: Experiment 1: Blastocyst rates of cloned embryos with gilt oocytes was about half compared with control. Experiment 2: An extended maturation time of 48 h used for gilt oocytes resulted in lower blastocyst rates after cloning. Experiment 3: Development of cloned embryos with gilt oocytes was improved by co-culture with sow oocytes. Experiment 4: After maturation of gilt oocytes using follicular fluid from gilt instead of sow, the oocytes were sorted into large and small oocytes, and after cloning, blastocyst rates were higher using large gilt oocytes compared with small oocytes; however, the rate remained lower compared with control. Experiment 5: Six sow recipients received a total of 503 morulae and blastocysts cloned from gilt oocytes (four recipients) and 190 cloned from sow oocytes (two recipients). All recipients became pregnant and went to term, resulting in 26 (gilt oocytes) and six (sow oocytes) piglets. In conclusion, results confirmed that nuclear transfer efficiency was higher using sow versus gilt oocytes, but the use of gilt oocytes can be optimized by sorting after ooplasm size following maturation and by maturing gilt and sow oocytes together.
authors: College of Animal Science and Technology,Nanjing Agricultural University,210095,Jiangsu Province,Nanjing,Wei Gang 1,China.
authors: Juan
authors: J
authors: Li
authors: Department of Animal Science,Aarhus University,Blichers Alle 20,DK-8830 Tjele,Denmark.
authors: Hanne Skovsgaard
authors: HS
authors: Pedersen
authors: Department of Animal Science,Aarhus University,Blichers Alle 20,DK-8830 Tjele,Denmark.
authors: Rong
authors: R
authors: Li
authors: Department of Animal Science,Aarhus University,Blichers Alle 20,DK-8830 Tjele,Denmark.
authors: Janne
authors: J
authors: Adamsen
authors: Department of Animal Science,Aarhus University,Blichers Alle 20,DK-8830 Tjele,Denmark.
authors: Ying
authors: Y
authors: Liu
authors: Veterinary Reproduction and Obstetrics,Faculty of Health and Medical Sciences,University of Copenhagen,DK-1870 Frederiksberg C,Denmark.
authors: Mette
authors: M
authors: Schmidt
authors: Department of Animal Science,Aarhus University,Blichers Alle 20,DK-8830 Tjele,Denmark.
authors: Stig
authors: S
authors: Purup
authors: Department of Animal Science,Aarhus University,Blichers Alle 20,DK-8830 Tjele,Denmark.
authors: Henrik
authors: H
authors: Callesen
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D019976:Cloning, Organism / Q000379:methods*; D018920:Coculture Techniques; D003593:Cytoplasm; D005260:Female; D059471:In Vitro Oocyte Maturation Techniques; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology*; D011247:Pregnancy; D011627:Puberty; D013552:Swine