title: Copper nanoclusters as peroxidase mimetics and their applications to H2O2 and glucose detection.
abstract: Copper nanoclusters (Cu NCs) are found to possess intrinsic peroxidase-like activity for the first time. Similar to nature peroxidase, they can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine by H(2)O(2) to produce a nice blue color reaction. Compared with horseradish peroxidase, Cu NCs exhibits higher activity near neutral pH, which is beneficial for biological applications. The increase in absorbance caused by the Cu NCs catalytic reaction allows the detection of H(2)O(2) in the range of 10 μM to 1 mM with a detection limit of 10 μM. A colorimetric method for glucose detection was also developed by combining the Cu NCs catalytic reaction and the enzymatic oxidation of glucose with glucose oxidase. Taking into account the advantages of ultra-small size, good stability, and high biocompatibility in aqueous solutions, Cu NCs are expected to have potential applications in biotechnology and clinical diagnosis as enzymatic mimics.
authors: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
authors: Lianzhe
authors: L
authors: Hu
authors: Yali
authors: Y
authors: Yuan
authors: Ling
authors: L
authors: Zhang
authors: Jianming
authors: J
authors: Zhao
authors: Saadat
authors: S
authors: Majeed
authors: Guobao
authors: G
authors: Xu
mesh_terms: D000818:Animals; D001560:Benzidines / Q000737:chemistry; D055162:Biocatalysis; D040761:Biomimetic Materials / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D003124:Colorimetry; D003300:Copper / Q000737:chemistry*; D005947:Glucose / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry; D007700:Kinetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D009195:Peroxidase / Q000378:metabolism*
chemical_list: D001560:Benzidines; C021758:3,3',5,5'-tetramethylbenzidine; D003300:Copper; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D009195:Peroxidase; D005947:Glucose