title: Biochemical and metabolic effects of a short-term exposure to nanoparticles of titanium silicate in tadpoles of Pelophylax perezi (Seoane).
abstract: This study aimed to evaluate sublethal effects of a short-term exposure (96 h) to titanium silicate nanoparticles (TiSiO(4)-NP) on Pelophylax perezi tadpoles. Tadpoles were exposed to five concentrations of TiSiO(4)-NP (8.2, 10.2, 12.8, 16 and 20 mg/L) plus a control. Effect criteria were: mortality, cholinesterases, glutathione S-transferases, lactate dehydrogenase, and catalase activities, and alanine and lactate contents. Light scattering was used for physical characterization of TiSiO(4)-NP suspensions, revealing a high aggregation state of the NP, consistent with low z-potential values (<30 mV). Mortality among TiSiO(4)-NP treatments was <11%. Significant differences relatively to the control were observed at the biochemical level (for CAT and LDH) and in lactate and alanine contents, which may end-up in increased oxidative stress. Overall, some of the monitored endpoints suggest metabolic alterations in TiSiO(4)-NP exposed tadpoles, highlighting the potential of TiSiO(4)-NP long-term effects on these organisms.
authors: Department of Biology, University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.
authors: T
authors: T
authors: Salvaterra
authors: M G
authors: MG
authors: Alves
authors: I
authors: I
authors: Domingues
authors: R
authors: R
authors: Pereira
authors: M G
authors: MG
authors: Rasteiro
authors: R A
authors: RA
authors: Carvalho
authors: A M V M
authors: AM
authors: Soares
authors: I
authors: I
authors: Lopes
mesh_terms: D000818:Animals; D007814:Larva / Q000187:drug effects; D053758:Nanoparticles / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D011898:Ranidae / Q000378:metabolism / Q000502:physiology*; D017640:Silicates / Q000633:toxicity*; D013997:Time Factors; D014025:Titanium / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D017640:Silicates; D014874:Water Pollutants, Chemical; C103828:titanium silicide; D014025:Titanium