title: Glutamine protects intestinal barrier function of colon epithelial cells from ethanol by modulating Hsp70 expression.
abstract: In this study, we investigated the protective effect of glutamine on barrier dysfunction induced by ethanol, by using human epithelial colorectal adenocarcinoma cells (Caco-2). Our results show that addition of glutamine to culture medium significantly improved the disruption of integrity caused by ethanol, which was associated with increased expression of heat shock protein 70 (Hsp70). Ethanol exposure moderately activates heat shock factor 1 (HSF1), which was characterized by increased DNA-binding activity and phosphorylation status of HSF1. Remarkably, glutamine treatment enhanced ethanol-mediated expression of Hsp70 and activation of HSF1. Up-regulation of Hsp70 by pretreatment with heat stress also promoted recovery from the ethanol-induced barrier dysfunction. Taken together, these observations indicate that glutamine protects the intestinal barrier function in Caco-2 cells, in part by modulating HSF1-mediated Hsp70 expression.
authors: Department of Pharmacy, Faculty of Pharmacy, Yasuda Women's University, Hiroshima, Japan. akagi@yasuda-u.ac.jp
authors: Reiko
authors: R
authors: Akagi
authors: Michiko
authors: M
authors: Ohno
authors: Kiminori
authors: K
authors: Matsubara
authors: Mitsuaki
authors: M
authors: Fujimoto
authors: Akira
authors: A
authors: Nakai
authors: Sachiye
authors: S
authors: Inouye
mesh_terms: D018938:Caco-2 Cells; D003106:Colon; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D000431:Ethanol; D005973:Glutamine / Q000494:pharmacology*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D000076249:Heat Shock Transcription Factors; D006801:Humans; D007444:Inulin / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D062826:Zonula Occludens-1 Protein / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; C000619706:HSF1 protein, human; D018840:HSP70 Heat-Shock Proteins; D000076249:Heat Shock Transcription Factors; C569042:TJP1 protein, human; D014157:Transcription Factors; D062826:Zonula Occludens-1 Protein; D005973:Glutamine; D000431:Ethanol; D007444:Inulin