title: Improved osteoblast cell affinity on plasma-modified 3-D extruded PCL scaffolds.
abstract: Cellular adhesion and proliferation inside three-dimensional synthetic scaffolds represent a major challenge in tissue engineering. Besides the surface chemistry of the polymers, it is well recognized that scaffold internal architecture, namely pore size/shape and interconnectivity, has a strong effect on the biological response of cells. This study reports for the first time how polycaprolactone (PCL) scaffolds with controlled micro-architecture can be effectively produced via bioextrusion and used to enhance the penetration of plasma deposited species. Low-pressure nitrogen-based coatings were employed to augment cell adhesion and proliferation without altering the mechanical properties of the structures. X-ray photoelectron spectroscopy carried out on different sections of the scaffolds indicates a uniform distribution of nitrogen-containing groups throughout the entire porous structure. In vitro biological assays confirm that plasma deposition sensitively promotes the activity of Saos-2 osteoblast cells, leading to a homogeneous colonization of the PCL scaffolds.
authors: Centre for Rapid and Sustainable Product Development, Polytechnic Institute of Leiria, Portugal.
authors: M
authors: M
authors: Domingos
authors: F
authors: F
authors: Intranuovo
authors: A
authors: A
authors: Gloria
authors: R
authors: R
authors: Gristina
authors: L
authors: L
authors: Ambrosio
authors: P J
authors: PJ
authors: Bártolo
authors: P
authors: P
authors: Favia
mesh_terms: D002448:Cell Adhesion; D002460:Cell Line; D019245:Compressive Strength; D055119:Elastic Modulus; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D058626:Plasma Gases / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering / Q000295:instrumentation*; D054457:Tissue Scaffolds
chemical_list: D058626:Plasma Gases; D011091:Polyesters; C016240:polycaprolactone