title: Interferon induction and suppression in swine testicle cells by porcine parvovirus and its proteins.
abstract: Porcine parvovirus (PPV) is a major causative agent of reproductive failure in swine, which currently affects the swine industry worldwide. Although PPV was identified several years ago, little is known about how it overcomes host innate immunity. In this study, we used quantitative real-time PCR and a luciferase reporter assay to determine whether PPV infection induces type I interferon (IFN-α and IFN-β) and whether PPV infection blocks dsRNA-induced IFN-β promoter activation in cell cultures. The results indicate that PPV does not induce type I interferon and that the NS2 protein of PPV could blocks dsRNA-induced IFN-β promoter activation.
authors: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001 Heilongjiang, China.
authors: Wencheng
authors: W
authors: Lin
authors: Zheng
authors: Z
authors: Qiu
authors: Qinfang
authors: Q
authors: Liu
authors: Shangjin
authors: S
authors: Cui
mesh_terms: D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D007372:Interferons / Q000235:genetics*; D008297:Male; D010322:Parvoviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D028323:Parvovirus, Porcine / Q000276:immunology*; D011070:Poly I-C / Q000276:immunology; D011401:Promoter Regions, Genetic / Q000235:genetics; D012330:RNA, Double-Stranded / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000821:virology; D013737:Testis / Q000166:cytology / Q000821:virology*; D013997:Time Factors; D014779:Virus Replication
chemical_list: D012330:RNA, Double-Stranded; D007372:Interferons; D011070:Poly I-C