title: Conformational stability of leukocyte lactate dehydrogenase in healthy men of different age.
abstract: Comparative analysis of cytosolic fraction distribution and conformational stability in lactate dehydrogenase (LDH) isozymes in healthy men of different age revealed significant differences indicating age-related characteristics of the enzyme. Activity of cytosolic fraction of LDH-1 and conformational stability of LDH-2 and LDH-3 in 40-year-old men were higher than in 20-year-olds. These data suggest age-related determination of conformational stability of individual LDH isozymes and functional heterogeneity of the enzyme within each isoform.
authors: Mental Health Research Institute, Siberian division of Russian Academy of Medical Sciences, Tomsk, Russia. f_o_y@mail.ru
authors: O Yu
authors: OY
authors: Fedorenko
authors: N A
authors: NA
authors: Bokhan
authors: S A
authors: SA
authors: Ivanova
mesh_terms: D000293:Adolescent; D000328:Adult; D000375:Aging; D003600:Cytosol / Q000737:chemistry; D004795:Enzyme Stability; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000737:chemistry* / Q000378:metabolism; D007962:Leukocytes / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D011487:Protein Conformation; D055815:Young Adult
chemical_list: D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; C409676:lactate dehydrogenase 2; C409677:lactate dehydrogenase 3; C409667:lactate dehydrogenase 1