title: Purification of high-complexity peptide microarrays by spatially resolved array transfer to gold-coated membranes.
abstract: A method for the one-step purification of high-complexity peptide microarrays is presented. The entire peptide library is transferred from the synthesis support to a gold coated polyvinylidenfluoride (PVDF) membrane, whereby only full-length peptides covalently couple to the receptor membrane via an N-terminally added cysteine. Highly resolved peptide transfer and purification of up to 10 000 features per cm(2) is demonstrated.
authors: German Cancer Research Center (DKFZ), Functional Genome Analysis, Chip-based Peptide Libraries, Heidelberg, Germany. c.schirwitz@dkfz.de
authors: Christopher
authors: C
authors: Schirwitz
authors: Felix F
authors: FF
authors: Loeffler
authors: Thomas
authors: T
authors: Felgenhauer
authors: Volker
authors: V
authors: Stadler
authors: Alexander
authors: A
authors: Nesterov-Mueller
authors: Reiner
authors: R
authors: Dahint
authors: Frank
authors: F
authors: Breitling
authors: F Ralf
authors: FR
authors: Bischoff