title: Porcine astrovirus viremia and high genetic variability in pigs on large holdings in Croatia.
abstract: Astroviruses are emerging viral agents, primarily enteropathogenic in mammals, but recently have been acknowledged to have extra-intestinal implications in humans and mink. Porcine astrovirus is thought to be widely distributed and highly prevalent among pigs, nevertheless its clinical significance remains doubtful as it can be detected in diarrheic as well as in healthy pigs. Recent reports imply the immense genetic variability among porcine astrovirus strains with five distinct lineages being characterized so far. Herein, we report porcine astrovirus circulation in the blood of healthy pigs in different age categories bred on two large industrial holdings in Croatia, with viral RNA seroprevalence of 3.89%. These are the first extra-intestinal findings of astrovirus in pigs, indicating a more complex pathogenesis than previously thought. Partial polymerase sequences of serum-derived strains provisionally clustered into porcine astrovirus lineages 2 and 4, sharing high genetic identity with previously described porcine astrovirus strains. The results were supported by detecting porcine astrovirus strains in composite fecal samples, regardless of pig category or holding tested. Phylogenetic analysis of derived strains suggested the presence of porcine astrovirus lineages previously detected in pig sera with an additional highly genetically divergent lineage 5, reported for the first time in Europe. Moreover, the existence of possible sub lineages should not be excluded. The results obtained in the present study, contribute to knowledge of porcine astrovirus pathogenesis; even though it's possible clinical significance remains unclear. High fecal prevalence accompanied with vast genetic diversity on a relatively confined area, underscores the importance of pigs as porcine astrovirus reservoirs with eventual recombination events as a possible outcome.
authors: Croatian Veterinary Institute, Virology Department, Savska cesta 143, 10000 Zagreb, Croatia. brnic@veinst.hr
authors: Dragan
authors: D
authors: Brnić
authors: Jelena
authors: J
authors: Prpić
authors: Tomislav
authors: T
authors: Keros
authors: Besi
authors: B
authors: Roić
authors: Vilim
authors: V
authors: Starešina
authors: Lorena
authors: L
authors: Jemeršić
mesh_terms: D000818:Animals; D019200:Astroviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D019350:Astroviridae Infections / Q000662:veterinary*; D017523:Croatia; D005243:Feces / Q000821:virology; D014644:Genetic Variation; D010802:Phylogeny; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D014766:Viremia / Q000662:veterinary*