title: Hollow fibers of poly(lactide-co-glycolide) and poly(ε-caprolactone) blends for vascular tissue engineering applications.
abstract: At present the manufacture of small-diameter blood vessels is one of the main challenges in the field of vascular tissue engineering. Currently available vascular grafts rapidly fail due to development of intimal hyperplasia and thrombus formation. Poly(lactic-co-glycolic acid) (PLGA) hollow fiber (HF) membranes have previously been proposed for this application, but as we show in the present work, they have an inhibiting effect on cell proliferation and rather poor mechanical properties. To overcome this we prepared HF membranes via phase inversion using blends of PLGA with poly(ε-caprolactone) (PCL). The influence of polymer composition on the HF physicochemical properties (topography, water transport and mechanical properties) and cell attachment and proliferation were studied. Our results show that only the ratio PCL/PLGA of 85/15 (PCL/PLGA85/15) yielded a miscible blend after processing. A higher PLGA concentration in the blend led to immiscible PCL/PLGA phase-separated HFs with an inhomogeneous morphology and variation in the cell culture results. In fact, the PCL/PLGA85/15 blend, which had the most homogeneous morphology and suitable pore structure, showed better human adipose stem cell (hASC) attachment and proliferation compared with the homopolymers. This, combined with the good mechanical and transport properties, makes them potentially useful for the development of small-caliber vascular grafts.
authors: MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Biomaterials Science and Technology Department, Faculty of Science and Technology, Enschede, The Netherlands.
authors: Nazely
authors: N
authors: Diban
authors: Suvi
authors: S
authors: Haimi
authors: Lydia
authors: L
authors: Bolhuis-Versteeg
authors: Sandra
authors: S
authors: Teixeira
authors: Susanna
authors: S
authors: Miettinen
authors: André
authors: A
authors: Poot
authors: Dirk
authors: D
authors: Grijpma
authors: Dimitrios
authors: D
authors: Stamatialis
mesh_terms: D001808:Blood Vessels; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008875:Middle Aged; D011091:Polyesters / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D023822:Tissue Engineering
chemical_list: D011091:Polyesters; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; C016240:polycaprolactone; D011100:Polyglycolic Acid; D019344:Lactic Acid