title: Autoantibody-induced internalization of nicotinic acetylcholine receptor α3 subunit exogenously expressed in human embryonic kidney cells.
abstract: Autoantibody against nicotinic acetylcholine receptor (nAChR) α3 subunit has been implicated in the pathogenesis of paraneoplastic neurological syndrome. To examine the effect of anti-α3 subunit autoantibody on cell-surface nAChRs, we established human embryonic kidney 293 cells stably co-expressing α3 and β4 subunits. Upon incubation with seropositive patient's serum, this cell line showed co-accumulation of patient's IgG and α3 subunits in the cytoplasm. These data support the hypothesis that anti-α3 subunit autoantibody induces internalization of cell-surface nAChRs and thereby impairs synaptic transmission.
authors: Laboratory of Alternative Medicine and Experimental Therapeutics, Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Ishikawa 920-1181, Japan.
authors: Shota
authors: S
authors: Kobayashi
authors: Shigeru
authors: S
authors: Yokoyama
authors: Takahiro
authors: T
authors: Maruta
authors: Masako
authors: M
authors: Negami
authors: Akiko
authors: A
authors: Muroyama
authors: Yasuhide
authors: Y
authors: Mitsumoto
authors: Kazuo
authors: K
authors: Iwasa
authors: Masahito
authors: M
authors: Yamada
authors: Hiroaki
authors: H
authors: Yoshikawa
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000502:physiology*; D019556:COS Cells; D002522:Chlorocebus aethiops; D004705:Endocytosis / Q000276:immunology*; D005786:Gene Expression Regulation / Q000276:immunology; D057809:HEK293 Cells; D006801:Humans; D051381:Rats; D011978:Receptors, Nicotinic / Q000378:metabolism*
chemical_list: D001323:Autoantibodies; D011978:Receptors, Nicotinic