title: A biodegradable and serum-resistant gene delivery carrier composed of polyamidoamine-poly N,N'-di-(2-aminoethyl) aminoethyl glutamine copolymer.
abstract: Polyamidoamine-poly N,N'-di-(2-aminoethyl) aminoethyl glutamine graft copolymers (PAMAM-PAGA) were synthesized by polymerization of BLG-NCA and subsequent aminolysis with tris-(2-aminoethyl)-amine. The chemical structure and composition of the copolymers were characterized by FT-IR and (1)H NMR. The physicochemical and biological performances of the copolymers or copolymer/pDNA complexes were evaluated by enzyme-degradation, agarose gel retardation, stability test against DNase I, DLS measurement, MTT assay, CLSM observation and in vitro transfection. The copolymers were biodegradable and less cytotoxic; the copolymers could self-assemble with pDNA to form complexes which possessed stability against DNase I; the particle sizes were in 140-200 nm and the zeta potentials of the complexes were in +10±0.6 mV; the complexes displayed the enhanced transfection efficiency and excellent serum-resistant capacity. Therefore, the PAMAM-PAGA copolymer can be used as a biodegradable and serum-resistant gene delivery carrier.
authors: The First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan 2 Road, Guangzhou 510080, PR China. gzpshr@163.com
authors: Shirong
authors: S
authors: Pan
authors: Duanwen
authors: D
authors: Cao
authors: Wu
authors: W
authors: Yi
authors: Huan
authors: H
authors: Huang
authors: Min
authors: M
authors: Feng
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry; D003850:Deoxyribonuclease I / Q000737:chemistry / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000737:chemistry*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D010957:Plasmids; D011073:Polyamines / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D011099:Polyglutamic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D058105:Polymerization; D013329:Structure-Activity Relationship
chemical_list: D010455:Peptides; D011073:Polyamines; C582967:polyamidoamine-poly N,N'-di-(2-aminoethyl)aminoethyl glutamine graft copolymer; C003172:poly-gamma-benzyl-L-glutamate; D011099:Polyglutamic Acid; D004247:DNA; D003850:Deoxyribonuclease I