title: Evaluating the use of Apo-neocarzinostatin as a cell penetrating protein.
abstract: Protein-ligand complex neocarzinostatin (NCS) is a small, thermostable protein-ligand complex that is able to deliver its ligand cargo into live mammalian cells where it induces DNA damage. Apo-NCS is able to functionally display complementarity determining regions loops, and has been hypothesised to act as a cell-penetrating protein, which would make it an ideal scaffold for cell targeting, and subsequent intracellular delivery of small-molecule drugs. In order to evaluate apo-NCS as a cell penetrating protein, we have evaluated the efficiency of its internalisation into live HeLa cells using matrix-assisted laser-desorption ionization-time-of-flight mass spectrometry and fluorescence microscopy. Following incubation of cells with apo-NCS, we observed no evidence of internalisation.
authors: Department of Chemistry, University College London, Gordon Street, London WC1H 0AJ, UK.
authors: Paul
authors: P
authors: Moody
authors: Fabienne
authors: F
authors: Burlina
authors: Stephen R
authors: SR
authors: Martin
authors: Rachel E
authors: RE
authors: Morgan
authors: John
authors: J
authors: Offer
authors: Mark E B
authors: ME
authors: Smith
authors: Justin E
authors: JE
authors: Molloy
authors: Stephen
authors: S
authors: Caddick
mesh_terms: D057846:Cell-Penetrating Peptides / Q000737:chemistry / Q000378:metabolism*; D004249:DNA Damage / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008024:Ligands; D008856:Microscopy, Fluorescence; D046912:Multiprotein Complexes / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D009353:Zinostatin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D057846:Cell-Penetrating Peptides; D008024:Ligands; D046912:Multiprotein Complexes; D009353:Zinostatin