title: Dietary extra virgin olive oil polyphenols supplementation modulates DSS-induced chronic colitis in mice.
abstract: We evaluated the protective effect of dietary extra virgin olive oil (EVOO) polyphenol extract (PE) supplementation in the inflammatory response associated to chronic colitis model. Six-week-old mice were randomized in four dietary groups: standard diet (SD), EVOO diet and both enriched with PE (850 ppm) (SD+PE and EVOO+PE). After 30 days, animals that were exposed to dextran sodium sulfate (DSS) (3%) followed by 3 weeks of drinking water developed chronic colitis, which was evaluated by disease activity index (DAI) and histology. Cell proliferation was analyzed by immunohistochemical and changes in monocyte chemotactic protein (MCP)-1 and tumor necrosis factor (TNF)-α mRNA expression by quantitative real-time polymerase chain reaction. Colonic expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, mitogen-activated protein kinases (MAPKs), IκBα inhibitory and peroxisome proliferator-activated receptor gamma (PPARγ) were determined by western blotting. SD-DSS group showed a significant increase of DAI, histological damage and cell proliferation, as well as an up-regulation of TNF-α, MCP-1, COX-2 and iNOS proteins. p38 and JNK MAPKs phosphorylation, IκBα degradation and PPARγ deactivation were also observed. However, in DSS-treated and EVOO+PE-fed mice, DAI and cell proliferation were significantly reduced, as well as MCP-1, TNF-α, COX-2 and iNOS expression levels. In addition, this dietary group, notably down-regulated JNK phosphorylation, prevented IκBα degradation and PPARγ deactivation. These results demonstrated, for the first time, that EVOO-PE supplementation possessed marked protective effects on experimental colitis through PPARγ up-regulation and nuclear transcription factor-kappa B and MAPK signaling pathway inhibition, decreasing the inflammatory cascade. We concluded that PE-enriched EVOO diet could be a beneficial functional food on ulcerative colitis.
authors: Department of Pharmacology, School of Pharmacy, University of Seville, 41012 Seville, Spain.
authors: Susana
authors: S
authors: Sánchez-Fidalgo
authors: Ana
authors: A
authors: Cárdeno
authors: Marina
authors: M
authors: Sánchez-Hidalgo
authors: Marina
authors: M
authors: Aparicio-Soto
authors: Catalina Alarcón
authors: CA
authors: de la Lastra
mesh_terms: D000818:Animals; D001483:Base Sequence; D002908:Chronic Disease; D003092:Colitis / Q000139:chemically induced / Q000517:prevention & control*; D017931:DNA Primers; D016264:Dextran Sulfate / Q000009:adverse effects*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry*; D059808:Polyphenols / Q000008:administration & dosage*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D000069463:Olive Oil; D010938:Plant Oils; D059808:Polyphenols; D016264:Dextran Sulfate