title: Identification and analysis of expressed genes using a cDNA library             from rat thymus during regeneration following cyclophosphamide-induced T cell             depletion.
abstract: Understanding the mechanisms of thymus regeneration is necessary for designing             strategies to enhance host immunity when immune function is suppressed due to             T cell depletion. In this study, expressed sequence tag (EST) analysis was performed             following generation of a regenerating thymus cDNA library to identify genes expressed             in thymus regeneration. A total of 1,000 ESTs were analyzed, of which 770 (77%)             matched to known genes, 178 matched to unknown genes (17.8%) and 52 (5.2%) did             not match any known sequences. The ESTs matched to known genes were grouped into             eight functional categories: gene/protein synthesis (28%), metabolism (24%), cell             signaling and communication (17%), cell structure and motility (6%), cell/organism             defense and homeostasis (6%), cell division (3%), cell death/apoptosis (2%), and             unclassified genes (14%). Based on the data of RT-PCR analysis, the expression             of TLP, E2IG2, pincher, Paip2, TGF-β1, 4-1BB and laminin α3 genes was increased             during thymus regeneration. These results provide extensive molecular information,             for the first time, on thymus regeneration indicating that the regenerating thymus             cDNA library may be a useful source for identifying various genes expressed during             thymus regeneration.
authors: Department of Anatomy, Pusan National University, School of Medicine,                     Yangsan, Gyeongsangnam-do 626-870, Republic of Korea.
authors: Tae-Deuk
authors: TD
authors: Yoon
authors: Hee-Woo
authors: HW
authors: Lee
authors: Yong-Sik
authors: YS
authors: Kim
authors: Hee Jung
authors: HJ
authors: Choi
authors: Jeon-Ok
authors: JO
authors: Moon
authors: Sik
authors: S
authors: Yoon
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003520:Cyclophosphamide; D020224:Expressed Sequence Tags; D015870:Gene Expression; D020869:Gene Expression Profiling; D015723:Gene Library; D008212:Lymphocyte Depletion; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000235:genetics*; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D060168:Thymocytes / Q000166:cytology*
chemical_list: D003520:Cyclophosphamide