title: Biofortified cassava increases β-carotene and vitamin A concentrations in the TAG-rich plasma layer of American women.
abstract: Biofortification of cassava with the provitamin A carotenoid β-carotene is a potential mechanism for alleviating vitamin A deficiency. Cassava is a staple food in the African diet, but data regarding the human bioavailability of β-carotene from this food are scarce. The objective of the present study was to evaluate provitamin A-enhanced cassava as a source of β-carotene and vitamin A for healthy adult women. The study was a randomised, cross-over trial of ten American women. The subjects consumed three different porridges separated by 2 week washout periods. Treatment meals (containing 100 g cassava) included: biofortified cassava (2 mg β-carotene) porridge with added oil (15 ml peanut or rapeseed oil, 20 g total fat); biofortified cassava porridge without added oil (6 g total fat); unfortified white cassava porridge with a 0·3 mg retinyl palmitate reference dose and added oil (20 g total fat). Blood was collected six times from - 0·5 to 9·5 h post-feeding. TAG-rich lipoprotein (TRL) plasma was separated by ultracentrifugation and analysed using HPLC with coulometric array electrochemical detection. The AUC for retinyl palmitate increased after the biofortified cassava meals were fed (P< 0·05). Vitamin A conversion was 4·2 (sd 3·1) and 4·5 (sd 3·1) μg β-carotene:1 μg retinol, with and without added oil, respectively. These results show that biofortified cassava increases β-carotene and retinyl palmitate TRL plasma concentrations in healthy well-nourished adult women, suggesting that it is a viable intervention food for preventing vitamin A deficiency.
authors: Western Human Nutrition Research Center, USDA-ARS-PWA, 430 West Health Sciences Drive, Davis, CA 95616, USA.
authors: Michael R
authors: MR
authors: La Frano
authors: Leslie R
authors: LR
authors: Woodhouse
authors: Dustin J
authors: DJ
authors: Burnett
authors: Betty J
authors: BJ
authors: Burri
mesh_terms: D000328:Adult; D019540:Area Under Curve; D004224:Diterpenes; D005260:Female; D005527:Food, Fortified; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D002365:Manihot / Q000737:chemistry*; D010938:Plant Oils / Q000494:pharmacology; D012016:Reference Values; D000084562:Retinyl Esters; D014280:Triglycerides / Q000097:blood*; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000097:blood; D014802:Vitamin A Deficiency / Q000097:blood* / Q000178:diet therapy; D055815:Young Adult; D019207:beta Carotene / Q000494:pharmacology*
chemical_list: D004224:Diterpenes; D008074:Lipoproteins; D010938:Plant Oils; D000084562:Retinyl Esters; D014280:Triglycerides; C030087:lipoprotein triglyceride; D019207:beta Carotene; D014801:Vitamin A; C014794:retinol palmitate