title: Reproducibility of first-pass cardiovascular magnetic resonance myocardial perfusion.
abstract: OBJECTIVE
  To assess the reproducibility of semiquantitative and quantitative analysis of first-pass myocardial perfusion cardiovascular magnetic resonance (CMR) in healthy volunteers.
  
  
  METHODS
  Eleven volunteers underwent myocardial perfusion CMR during adenosine stress and rest on 2 separate days. Perfusion data were acquired in a single mid-ventricular section in two cardiac phases to permit cardiac phase reproducibility comparisons. Semiquantitative analysis was performed to derive normalized upslopes of myocardial signal intensity profiles (myocardial perfusion index, MPI). The quantitative analysis estimated absolute myocardial blood flow (MBF) using Fermi-constrained deconvolution. The perfusion reserve index was calculated by dividing stress by rest data. Two observers performed all the measurements independently. One observer repeated all first scan measurements 4 weeks later.
  
  
  RESULTS
  The reproducibility of perfusion CMR was highest for semiquantitative analysis with an intraobserver coefficient of variability (CoV) of 3%-7% and interobserver CoV of 4%-10%. Semiquantitative interstudy comparison was less reproducible (CoV of 13%-27%). Quantitative intraobserver CoV of 10%-18%, interobserver CoV of 8%-15% and interstudy CoV of 20%-41%. Reproducibility of systolic and diastolic phases and the endocardial and epicardial myocardial layer showed similar reproducibility on both semiquantitative and quantitative analysis.
  
  
  CONCLUSIONS
  The reproducibility of CMR myocardial perfusion estimates is good, but varies between intraobserver, interobserver, and interstudy comparisons. In this study semiquantitative analysis was more reproducible than quantitative analysis.
authors: Multidisciplinary Cardiovascular Research Centre & Leeds Institute of Genetics, Health and Therapeutics, University of Leeds, Leeds, UK.
authors: Abdulghani M
authors: AM
authors: Larghat
authors: Neil
authors: N
authors: Maredia
authors: John
authors: J
authors: Biglands
authors: John P
authors: JP
authors: Greenwood
authors: Stephen G
authors: SG
authors: Ball
authors: Michael
authors: M
authors: Jerosch-Herold
authors: Aleksandra
authors: A
authors: Radjenovic
authors: Sven
authors: S
authors: Plein
mesh_terms: D000328:Adult; D003326:Coronary Circulation / Q000502:physiology; D005260:Female; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D018810:Magnetic Resonance Angiography / Q000379:methods*; D008297:Male; D055414:Myocardial Perfusion Imaging / Q000379:methods*; D015588:Observer Variation; D012016:Reference Values; D015203:Reproducibility of Results; D016277:Ventricular Function, Left / Q000502:physiology; D015646:Ventriculography, First-Pass / Q000379:methods*