title: Multiplex flow cytometry barcoding and antibody arrays identify surface antigen profiles of primary and metastatic colon cancer cell lines.
abstract: Colon cancer is a deadly disease affecting millions of people worldwide. Current treatment challenges include management of disease burden as well as improvements in detection and targeting of tumor cells. To identify disease state-specific surface antigen signatures, we combined fluorescent cell barcoding with high-throughput flow cytometric profiling of primary and metastatic colon cancer lines (SW480, SW620, and HCT116). Our multiplexed technique offers improvements over conventional methods by permitting the simultaneous and rapid screening of cancer cells with reduced effort and cost. The method uses a protein-level analysis with commercially available antibodies on live cells with intact epitopes to detect potential tumor-specific targets that can be further investigated for their clinical utility. Multiplexed antibody arrays can easily be applied to other tumor types or pathologies for discovery-based approaches to target identification.
authors: Department of Stem Cell Biology and Regenerative Medicine, Cleveland Clinic Lerner Research Institute, Cleveland, OH, USA. kumar@case.edu
authors: Kumar
authors: K
authors: Sukhdeo
authors: Rosanto I
authors: RI
authors: Paramban
authors: Jason G
authors: JG
authors: Vidal
authors: Jeanne
authors: J
authors: Elia
authors: Jody
authors: J
authors: Martin
authors: Maricruz
authors: M
authors: Rivera
authors: Daniel R
authors: DR
authors: Carrasco
authors: Awad
authors: A
authors: Jarrar
authors: Matthew F
authors: MF
authors: Kalady
authors: Christian T
authors: CT
authors: Carson
authors: Robert
authors: R
authors: Balderas
authors: Anita B
authors: AB
authors: Hjelmeland
authors: Justin D
authors: JD
authors: Lathia
authors: Jeremy N
authors: JN
authors: Rich
mesh_terms: D000941:Antigens / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D045744:Cell Line, Tumor; D003106:Colon / Q000473:pathology*; D003110:Colonic Neoplasms / Q000473:pathology*; D019295:Computational Biology / Q000379:methods / Q000458:organization & administration; D005434:Flow Cytometry / Q000379:methods*; D005455:Fluorescent Antibody Technique / Q000379:methods; D057166:High-Throughput Screening Assays / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D009362:Neoplasm Metastasis / Q000473:pathology*; D040081:Protein Array Analysis / Q000379:methods*; D014407:Tumor Cells, Cultured
chemical_list: D000941:Antigens; D014408:Biomarkers, Tumor