title: Genetic analysis of RNA1 and RNA2 of Macrobrachium rosenbergii nodavirus (MrNV) isolated from India.
abstract: Macrobrachium rosenbergii nodavirus (MrNV) is responsible for the newly emerging catastrophic disease known as white tail disease (WTD) in M. rosenbergii. The complete sequence of RNA2 (1175 bp) and 3126 bp region of RNA1 of an Indian strain of MrNV was generated. Sequence analysis of RNA2 revealed the presence of a single ORF encoding a capsid protein of 371 amino acids with a predicted molecular mass and pI of 41.5 kDa and 8.97 respectively. RNA1 contained two ORFs, one encoding a partial RNA dependent RNA polymerase (RdRp) of length 1034 amino acids and another a B2-like protein with a length 133 amino acids. A phylogenetic analysis based on the amino acid sequence of the capsid protein, to related nodavirus sequences suggests the establishment of new genotypes within the Nodaviridae family and we suggest the name should be genus Gammanodavirus. A new reverse transcriptase-polymerase chain reaction (RT-PCR) assay has been developed and optimized for the detection of shrimp nodavirus with a sensitivity to detect up to 24 copy numbers of plasmid construct.
authors: UNESCO-MIRCEN for Marine Biotechnology, Department of Fisheries Microbiology, Karnataka Veterinary, Animal and Fisheries Sciences University, College of Fisheries, Mangalore 575002, India.
authors: Singaiah
authors: S
authors: NaveenKumar
authors: Malathi
authors: M
authors: Shekar
authors: Iddya
authors: I
authors: Karunasagar
authors: Indrani
authors: I
authors: Karunasagar
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D016679:Genome, Viral; D007194:India; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008970:Molecular Weight; D030381:Nodaviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016366:Open Reading Frames; D033541:Palaemonidae / Q000821:virology*; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins