title: Fast microwave-assisted extraction of rotenone for its quantification in seeds of yam bean (Pachyrhizus sp.).
abstract: The aim of this study was to find if fast microwave-assisted extraction could be an alternative to the conventional Soxhlet extraction for the quantification of rotenone in yam bean seeds by SPE and HPLC-UV. For this purpose, an experimental design was used to determine the optimal conditions of the microwave extraction. Then the values of the quantification on three accessions from two different species of yam bean seeds were compared using the two different kinds of extraction. A microwave extraction of 11 min at 55°C using methanol/dichloromethane (50:50) allowed rotenone extraction either equivalently or more efficiently than the 8-h-Soxhlet extraction method and was less sensitive to moisture content. The selectivity, precision, trueness, accuracy, and limit of quantification of the method with microwave extraction were also demonstrated.
authors: Laboratoire de Pharmacognosie, LDRI, Université Catholique de Louvain, Brussels, Belgium. emmanuelle.lautie@uclouvain.be
authors: Emmanuelle
authors: E
authors: Lautié
authors: Catherine
authors: C
authors: Rasse
authors: Eric
authors: E
authors: Rozet
authors: Claire
authors: C
authors: Mourgues
authors: Jean-Paul
authors: JP
authors: Vanhelleputte
authors: Joëlle
authors: J
authors: Quetin-Leclercq
mesh_terms: D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D008872:Microwaves; D029905:Pachyrhizus / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification*; D012402:Rotenone / Q000032:analysis* / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry
chemical_list: D010936:Plant Extracts; D012402:Rotenone