title: [Analyze on the expression differences of key genes in saponins biosynthesize in Eleutherococcus senticosus and its callus].
abstract: OBJECTIVE
  To analyze the expression differences of SS, SE and bAS in leaf, petiole and callus from leaf and petiole of Eleutherococcus senticosus.
  
  
  METHODS
  Calluses were induced from explants of E. senticosus leaf and petiole. The expression amount of SS, SE and bAS gene was detected by real time PCR.
  
  
  RESULTS
  The expression amount of SS, SE and bAS genes in callus from leaf were 89.3%, 73.8% and 83.4% of that in leaf, respectively. The expression amount of SS, SE and bAS genes in callus from petiole were 80.2%, 87.7% and 70.9% of that in petiole, respectively.
  
  
  CONCLUSIONS
  The expression amount of SS, SE and bAS gene in callus from E. senticosus are significantly lower than that in plant.
authors: College of Life Science, Hebei United University, Tangshan 063000, China. xingzhaobin@yahoo.com.cn
authors: Zhao-Bin
authors: ZB
authors: Xing
authors: Peng
authors: P
authors: Wu
authors: Yue-Hong
authors: YH
authors: Long
authors: Shan
authors: S
authors: He
authors: Neng-Song
authors: NS
authors: Liang
mesh_terms: D028001:Eleutherococcus / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D013186:Farnesyl-Diphosphate Farnesyltransferase / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D019751:Intramolecular Transferases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D027343:Rhizome / Q000378:metabolism; D012503:Saponins / Q000032:analysis / Q000096:biosynthesis*; D050603:Squalene Monooxygenase / Q000235:genetics* / Q000378:metabolism
chemical_list: D012503:Saponins; D050603:Squalene Monooxygenase; D013186:Farnesyl-Diphosphate Farnesyltransferase; D019751:Intramolecular Transferases