title: Immunosuppressive monoclonal antibody to CD64 from patients with long-term stable multiple sclerosis.
abstract: High intrathecal levels of anti-myelin basic protein (MBP) IgM were previously found to be significantly associated with early favorable course in a cohort of patients with multiple sclerosis (MS). A mAb to MBP 105-120 recognizing the 222-228 epitope of the extracellular domain of high affinity immunoglobulin gamma Fc-receptor I (CD64) was isolated from EBV(+) B cell clones of long-term stable RRMS patients. This mAb exerted immunosuppressive activity on MS-derived T cell lines through induction and release of high amounts of interleukin-10 and decreased levels of interleukin-12 from activated monocytes providing the biological basis for a potential new treatment for MS and other immune-mediated neurological disorders.
authors: Department of Medicine, Surgery and Neuroscience, University of Siena, Siena, Italy. annunziata@unisi.it
authors: P
authors: P
authors: Annunziata
authors: C
authors: C
authors: Cioni
authors: L
authors: L
authors: Cantalupo
authors: G
authors: G
authors: Di Genova
authors: G Gori
authors: GG
authors: Savellini
authors: G
authors: G
authors: Cusi
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal / Q000097:blood / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D015331:Cohort Studies; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D047468:Immunoprecipitation; D007166:Immunosuppressive Agents / Q000097:blood / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000473:pathology*; D004676:Myelin Basic Protein / Q000276:immunology*; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000737:chemistry / Q000276:immunology; D010455:Peptides / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism; D017452:Receptors, IgG / Q000235:genetics / Q000378:metabolism*; D018709:Statistics, Nonparametric; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D014157:Transcription Factors / Q000276:immunology; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D016207:Cytokines; D004268:DNA-Binding Proteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007166:Immunosuppressive Agents; C078341:MYT1 protein, human; D004676:Myelin Basic Protein; D063308:Myelin-Oligodendrocyte Glycoprotein; D010455:Peptides; D012333:RNA, Messenger; D017452:Receptors, IgG; D014157:Transcription Factors