title: Decreased expression of HOXB9 is related to poor overall survival in patients with gastric carcinoma.
abstract: BACKGROUND
  Studies have demonstrated the implication of HOXB9 in tumorigenesis, but its role in gastric carcinoma remains unknown.
  
  
  OBJECTIVE
  To investigate the expression and prognostic value of HOXB9 in patients with gastric carcinoma.
  
  
  METHODS
  The localization and expression of HOXB9 in gastric cancer cells lines were detected by immunofluorescence and western blot. The mRNA and protein expression level of HOXB9 was detected in subjects with gastric carcinoma and paired non-cancerous tissues. Correlation between HOXB9 expression and clinicopathological parameters, the association of HOXB9 expression with the patients' survival rate was also assessed.
  
  
  RESULTS
  HOXB9 was predominantly localized in the cell nucleus. A significant decrease in HOXB9 intensity in poorly differentiated gastric cancer cells is evident (P<0.01). A lower mRNA and protein expression level of HOXB9 was detected in gastric carcinoma (P<0.01). Decreased expression of HOXB9, poorly differentiation status and the presence of lymph node metastasis predict shorter overall survival (P<0.05). Patients without HOXB9 expression had a lower overall survival rate (P<0.01). Multivariate Cox regression analysis showed HOXB9 was an independent prognostic factor in gastric carcinoma (P<0.01).
  
  
  CONCLUSIONS
  HOXB9 is down-regulation in gastric carcinoma and may be a novel prognostic marker for poorer clinical outcome for patients with gastric carcinoma.
authors: State Key Laboratory of Cancer Biology & Xijing Hospital of Digestive Diseases, The Fourth Military Medical University, Xi'an, Shaanxi Province, PR China. shasumei001@163.com
authors: Sumei
authors: S
authors: Sha
authors: Yong
authors: Y
authors: Gu
authors: Bin
authors: B
authors: Xu
authors: Hao
authors: H
authors: Hu
authors: Yan
authors: Y
authors: Yang
authors: Xiangyun
authors: X
authors: Kong
authors: Kaichun
authors: K
authors: Wu
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D002277:Carcinoma / Q000378:metabolism* / Q000401:mortality*; D045744:Cell Line, Tumor; D005260:Female; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D018613:Microscopy, Confocal; D008875:Middle Aged; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000401:mortality*; D016019:Survival Analysis; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor; C491257:HOXB9 protein, human; D018398:Homeodomain Proteins