title: Expression and biological function of programmed death ligands in human placenta mesenchymal stem cells.
abstract: Mesenchymal stem cells (MSCs) play important roles in tissue regeneration due to their self-renewal, multilineage differentiation and immunosuppression abilities. MSCs can be isolated from various kinds of tissue, such as umbilical cord, cord blood and placenta. Human placenta mesenchymal stem cells (hPMSCs) possess stronger immunosuppressive properties, such as the ability to inhibit T-cell activation and proliferation, than human bone marrow MSCs. We have investigated that the roles of the programmed death ligands 1 and 2 (PDL1 and PDL2) in hPMSC adhesion, migration and immunosuppression were investigated. PDL1 and PDL2 were highly expressed by hPMSCs. Knockdown of PDL1 and/or PDL2 by siRNA increased hPMSC adhesion, but greatly decreasing migration. PDL1 and PDL2 expressed on hPMSCs inhibited T-cell proliferation by arresting the cell cycle. Knockdown of PDL1 and/or PDL2 in hPMSCs, however, had no effect on the expression of CD69, a T-cell early activation marker found on both CD4(+)  and CD8(+)  T-cell subsets. In summary, the roles of the negative co-stimulators PDL1 and PDL2 is on the adhesion, migration and immunosuppression of hPMSCs. These findings may be useful regarding the potential use of hPMSCs in clinical cell.
authors: Department of Immunology, Binzhou Medical University, Shandong Province, Yantai 264003, P.R. China.
authors: Guoyan
authors: G
authors: Wang
authors: Siying
authors: S
authors: Zhang
authors: Feifei
authors: F
authors: Wang
authors: Guangyun
authors: G
authors: Li
authors: Lixia
authors: L
authors: Zhang
authors: Xiying
authors: X
authors: Luan
mesh_terms: D001343:Autophagy / Q000502:physiology; D060890:B7-H1 Antigen / Q000235:genetics / Q000378:metabolism; D016923:Cell Death; D002454:Cell Differentiation; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D008024:Ligands; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010920:Placenta / Q000166:cytology* / Q000378:metabolism; D011247:Pregnancy; D016176:T-Lymphocyte Subsets / Q000276:immunology
chemical_list: D060890:B7-H1 Antigen; C423236:CD274 protein, human; D008024:Ligands