title: Nucleosome mobilization by ISW2 requires the concerted action of the ATPase and SLIDE domains.
abstract: The ISWI family of ATP-dependent chromatin remodelers represses transcription by changing nucleosome positions. ISWI regulates nucleosome positioning by requiring a minimal length of extranucleosomal DNA for moving nucleosomes. ISW2 from Saccharomyces cerevisiae, a member of the ISWI family, has a conserved domain called SLIDE (SANT-like ISWI domain) that binds to extranucleosomal DNA ~19 base pairs from the edge of nucleosomes. Loss of SLIDE binding does not perturb binding of the ATPase domain or the initial movement of DNA inside of nucleosomes. Not only is extranucleosomal DNA required to help recruit ISW2, but also the interactions of the SLIDE domain with extranucleosomal DNA are functionally required to move nucleosomes.
authors: Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, Illinois, USA.
authors: Swetansu K
authors: SK
authors: Hota
authors: Saurabh K
authors: SK
authors: Bhardwaj
authors: Sebastian
authors: S
authors: Deindl
authors: Yuan-chi
authors: YC
authors: Lin
authors: Xiaowei
authors: X
authors: Zhuang
authors: Blaine
authors: B
authors: Bartholomew
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D015153:Blotting, Western; D002499:Centrifugation, Density Gradient; D042002:Chromatin Assembly and Disassembly / Q000235:genetics / Q000502:physiology*; D004271:DNA, Fungal / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer; D015966:Gene Expression Regulation, Fungal / Q000235:genetics / Q000502:physiology*; D017665:Hydroxyl Radical / Q000378:metabolism; D009707:Nucleosomes / Q000378:metabolism*; D019920:Photoaffinity Labels; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D004271:DNA, Fungal; C086468:ISWI protein; D009707:Nucleosomes; D019920:Photoaffinity Labels; D014157:Transcription Factors; D017665:Hydroxyl Radical; D000251:Adenosine Triphosphatases