title: Quantitative real-time RT-PCR of ITGA7, SVEP1, TNS1, LPHN3, SEMA3G, KLB and MMP13 mRNA expression in breast cancer.
abstract: Breast cancer is the leading cause of cancer deaths among women worldwide, including Thailand. In the present study, the differential mRNA expression of SVEP1, LPHN3, KLB, ITGA7, SEMA3G, TNS1 and MMP13 genes was examined in breast cancer using quantitative real-time reverse transcription polymerase chain reaction (QRT-PCR). Among these genes, increased LPHN3 and MMP13 mRNA expression levels correlated with axillary-node metastasis (P=0.02). Multiple logistic regression analysis revealed that LPHN3 and MMP13 mRNA expression is significantly associated with axillary node status in breast cancer (P=0.04).
authors: Department of Tropical Nutrition and Food Science, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors: Manas
authors: M
authors: Kotepui
authors: Charin
authors: C
authors: Thawornkuno
authors: Porntip
authors: P
authors: Chavalitshewinkoon-Petmitr
authors: Phaibul
authors: P
authors: Punyarit
authors: Songsak
authors: S
authors: Petmitr
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000235:genetics; D001365:Axilla; D014408:Biomarkers, Tumor / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D039001:Integrin alpha Chains / Q000235:genetics; D008207:Lymphatic Metastasis; D053509:Matrix Metalloproteinase 13 / Q000235:genetics; D008840:Microfilament Proteins / Q000235:genetics; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D043562:Receptors, G-Protein-Coupled / Q000235:genetics; D018000:Receptors, Peptide / Q000235:genetics; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D039961:Semaphorins / Q000235:genetics; D000070876:Tensins; D013785:Thailand
chemical_list: C513284:ADGRL3 protein, human; D015703:Antigens, CD; D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D039001:Integrin alpha Chains; D008840:Microfilament Proteins; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D018000:Receptors, Peptide; C508516:SVEP1 protein, human; D039961:Semaphorins; C000603369:TNS1 protein, human; D000070876:Tensins; C085725:integrin alpha7; C569384:semaphorin 3G, human; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13