title: Purification and characterization of a novel acid phosphatase from the split gill mushroom Schizophyllum commune.
abstract: A monomeric acid phosphatase (ACP) with a molecular mass of 72.5 kDa was purified from fresh fruiting bodies of cultured Schizophyllum commune mushroom. The isolation procedure entailed ion exchange chromatography on DEAE-cellulose, CM-cellulose, and Q-sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. It demonstrated a unique N-terminal amino acid sequence of NAPWAQIDEV, which exhibited 60% amino acid identity to that of S. commune hypothetical histidine ACP based on its genome sequence, but less than 30% amino acid identity to that of other fungal ACPs previously reported. The ACP exhibited an optimum temperature at 50 °C, an optimum pH at pH 4.6, and was considerably stable at a pH range of 4.0 to 9.0, and a temperature range of 20-40 °C. The Km of the purified enzyme for ρ-nitrophenyl phosphate (ρNPP) was 0.248 mM and the Vmax was 9.093 × 10(-3)  μM/min. ACP activity was strongly inhibited by Al(3+) and Fe(3+) , but enhanced by Co(2+) , Mg(2+) , and Ca(2+) at a concentration of 0.5 mM.
authors: Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, Beijing University of Agriculture, Beijing, China.
authors: Guo-Qing
authors: GQ
authors: Zhang
authors: Qing-Jun
authors: QJ
authors: Chen
authors: Jian
authors: J
authors: Sun
authors: He-Xiang
authors: HX
authors: Wang
authors: Chun-Hua
authors: CH
authors: Han
mesh_terms: D000135:Acid Phosphatase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D048690:Fruiting Bodies, Fungal / Q000201:enzymology* / Q000502:physiology; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D012567:Schizophyllum / Q000201:enzymology*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D005656:Fungal Proteins; D000135:Acid Phosphatase
keywords: Acid phosphatase; Chromatography; Kinetics; N-terminal amino acid sequence; Schizophyllum commune