title: TokyoGreen derivatives as specific and practical fluorescent probes for UDP-glucuronosyltransferase (UGT) 1A1.
abstract: TokyoGreen (TG) derivatives were found to be efficient and specific substrates of an important drug-metabolizing enzyme, UDP-glucuronosyltransferase (UGT) 1A1. A rapid, specific, and sensitive assay of the enzyme was achieved simply by monitoring the change in fluorescence intensity. We also designed and developed the first "turn-on" fluorescent probes for UGTs.
authors: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Tokyo, Japan.
authors: Takuya
authors: T
authors: Terai
authors: Rie
authors: R
authors: Tomiyasu
authors: Tomoe
authors: T
authors: Ota
authors: Tasuku
authors: T
authors: Ueno
authors: Toru
authors: T
authors: Komatsu
authors: Kenjiro
authors: K
authors: Hanaoka
authors: Yasuteru
authors: Y
authors: Urano
authors: Tetsuo
authors: T
authors: Nagano
mesh_terms: D000069446:Atazanavir Sulfate; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D005452:Fluoresceins / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000378:metabolism; D015394:Molecular Structure; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology; D011725:Pyridines / Q000737:chemistry / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D000099148:UGT1A1 Enzyme
chemical_list: D000069446:Atazanavir Sulfate; D004791:Enzyme Inhibitors; D005452:Fluoresceins; D005456:Fluorescent Dyes; D014453:Glucuronosyltransferase; D009842:Oligopeptides; D011725:Pyridines; D000099148:UGT1A1 Enzyme; C521239:TokyoGreen-NPE AM