title: End-of-life cell cycle arrest contributes to stochasticity of yeast replicative aging.
abstract: There is growing evidence that stochastic events play an important role in determining individual longevity. Studies in model organisms have demonstrated that genetically identical populations maintained under apparently equivalent environmental conditions display individual variation in life span that can be modeled by the Gompertz-Makeham law of mortality. Here, we report that within genetically identical haploid and diploid wild-type populations, shorter-lived cells tend to arrest in a budded state, while cells that arrest in an unbudded state are significantly longer-lived. This relationship is particularly notable in diploid BY4743 cells, where mother cells that arrest in a budded state have a shorter mean life span (25.6 vs. 35.6) and larger coefficient of variance with respect to individual life span (0.42 vs. 0.32) than cells that arrest in an unbudded state. Mutations that cause genomic instability tend to shorten life span and increase the proportion of the population that arrest in a budded state. These observations suggest that randomly occurring damage may contribute to stochasticity during replicative aging by causing a subset of the population to terminally arrest prematurely in the S or G2 phase of the cell cycle.
authors: Department of Pathology, University of Washington, Seattle, WA 98195-7470, USA.
authors: Joe R
authors: JR
authors: Delaney
authors: Annie
authors: A
authors: Chou
authors: Brady
authors: B
authors: Olsen
authors: Daniel
authors: D
authors: Carr
authors: Christopher
authors: C
authors: Murakami
authors: Umema
authors: U
authors: Ahmed
authors: Sylvia
authors: S
authors: Sim
authors: Elroy H
authors: EH
authors: An
authors: Anthony S
authors: AS
authors: Castanza
authors: Marissa
authors: M
authors: Fletcher
authors: Sean
authors: S
authors: Higgins
authors: Mollie
authors: M
authors: Holmberg
authors: Jessica
authors: J
authors: Hui
authors: Monika
authors: M
authors: Jelic
authors: Ki-Soo
authors: KS
authors: Jeong
authors: Jin R
authors: JR
authors: Kim
authors: Shannon
authors: S
authors: Klum
authors: Eric
authors: E
authors: Liao
authors: Michael S
authors: MS
authors: Lin
authors: Winston
authors: W
authors: Lo
authors: Hillary
authors: H
authors: Miller
authors: Richard
authors: R
authors: Moller
authors: Zhao J
authors: ZJ
authors: Peng
authors: Tom
authors: T
authors: Pollard
authors: Prarthana
authors: P
authors: Pradeep
authors: Dillon
authors: D
authors: Pruett
authors: Dilreet
authors: D
authors: Rai
authors: Vanessa
authors: V
authors: Ros
authors: Jennifer
authors: J
authors: Schleit
authors: Alex
authors: A
authors: Schuster
authors: Minnie
authors: M
authors: Singh
authors: Benjamin L
authors: BL
authors: Spector
authors: George L
authors: GL
authors: Sutphin
authors: Adrienne M
authors: AM
authors: Wang
authors: Brian M
authors: BM
authors: Wasko
authors: Helen
authors: H
authors: Vander Wende
authors: Brian K
authors: BK
authors: Kennedy
authors: Matt
authors: M
authors: Kaeberlein
mesh_terms: D059447:Cell Cycle Checkpoints; D050296:Microbial Viability; D013269:Stochastic Processes; D015003:Yeasts / Q000502:physiology*