title: Gobichelin A and B: Mixed-Ligand Siderophores Discovered Using Proteomics.
abstract: "Omic" strategies have been increasingly applied to natural product discovery processes, with (meta-)genome sequencing and mining implemented in many laboratories to date. Using the proteomics-based discovery platform called PrISM (Proteomic Investigation of Secondary Metabolism), we discovered two new siderophores gobichelin A and B from Streptomyces sp. NRRL F-4415, a strain without a sequenced genome. Using the proteomics information as a guide, the 37 kb gene cluster responsible for production of gobichelins was sequenced and its 20 open reading frames interpreted into a biosynthetic scheme. This led to the targeted detection and structure elucidation of the new compounds produced by nonribosomal peptide (NRP) synthesis.
authors: Department of Chemistry and Chemistry of Life Processes Institute, Northwestern University, 2145 Sheridan Road, Evanston, IL, 60208.
authors: Yunqiu
authors: Y
authors: Chen
authors: Michelle
authors: M
authors: Unger
authors: Ioanna
authors: I
authors: Ntai
authors: Ryan A
authors: RA
authors: McClure
authors: Jessica C
authors: JC
authors: Albright
authors: Regan J
authors: RJ
authors: Thomson
authors: Neil L
authors: NL
authors: Kelleher