title: Pro-inflammatory cytokines in saliva of adolescents with dental caries disease.
abstract: OBJECTIVE
  Dental caries is an inflammatory disease with multifactorial etiology. The presented study was conducted to test the hypothesis that the elevation of salivary cytokines - interleukin-6 (IL-6), interleukin-8 (IL-8) and tumour necrosis factor (TNF-a) is changed in dental caries patients. IL-6, IL-8 and TNF-a are particularly relevant to inflammation, one of the very first responses of the host to a pathological insult.
  
  
  METHODS
  Whole saliva from 26 patients with dental caries, as well as 10 healthy persons, was investigated for the presence of IL-6, IL-8 and TNF-a by enzyme immunoassay - ELISA.
  
  
  RESULTS
  The results showed that an elevation of IL-6, IL-8 and TNF-a in unstimulated whole saliva in subjects with dental caries, compared with controls, increased and was statistically significant in all cases (p <0.05). The study also show a positive correlation between TNF-a and IL-8.
  
  
  CONCLUSIONS
  These data suggest links between the production of tumour necrosis factor (TNF-a), interleukin-6 (IL-6), interleukin-8 (IL-8) in saliva and dental caries disease.
authors: Department of Biotechnology, Medical University, Bialystok, Poland.
authors: Agnieszka
authors: A
authors: Gornowicz
authors: Anna
authors: A
authors: Bielawska
authors: Krzysztof
authors: K
authors: Bielawski
authors: Stanisława Zyta
authors: SZ
authors: Grabowska
authors: Anna
authors: A
authors: Wójcicka
authors: Magdalena
authors: M
authors: Zalewska
authors: Elżbieta
authors: E
authors: Maciorkowska
mesh_terms: D000293:Adolescent; D015415:Biomarkers / Q000032:analysis; D003731:Dental Caries / Q000175:diagnosis* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism*; D011044:Poland; D012463:Saliva / Q000737:chemistry*; D018709:Statistics, Nonparametric; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D015850:Interleukin-6; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha