title: Universally increased mRNA stability downstream of the translation initiation site in eukaryotes and prokaryotes.
abstract: Local secondary structures in coding sequences have important functions across various translational processes. To date, however, the local structures and their functions in the early stage of translation elongation remain poorly understood. Here, we surveyed the structural stability in the first 180 nucleotides of the coding sequence of 27 species using computational method. We found that the structural stability in the 30-80 nucleotide interval was significantly higher than that in other regions in eukaryotes and most prokaryotes. No significant correlation between local translation efficiency and structural stability was observed, suggesting that this structural region has undergone selection pressure directly to maintain high stability. Furthermore, ribosome was blocked by this region, providing an opportunity for co-translational regulation. Remarkably, in eukaryotes, we found that mRNAs with higher structural stability in the 30-80 nucleotide interval tended to encode the secreted proteins. Overall, our results revealed a previously unappreciated correlation between structural stability and protein localization.
authors: State Key Laboratory of Crop Stress Biology in Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors: Yuanhui
authors: Y
authors: Mao
authors: Wangtian
authors: W
authors: Wang
authors: Nan
authors: N
authors: Cheng
authors: Qian
authors: Q
authors: Li
authors: Shiheng
authors: S
authors: Tao
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D056890:Eukaryota / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009690:Nucleic Acid Conformation; D010944:Plants / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis; D020871:RNA Stability; D012333:RNA, Messenger / Q000737:chemistry*; D012270:Ribosomes
chemical_list: D012333:RNA, Messenger