title: The efficacy of L. (L.) chagasi excreted-secreted antigens (ESAs) for visceral leishmaniasis diagnosis is due to low levels of cross-reactivity.
abstract: The analysis of promastigote excreted-secreted antigen (ESA) reactivity with 53 visceral leishmaniasis (VL) cases showed that each sample reacted regardless of the antigen or the Leishmania species used in enzyme-linked immunosorbent assay (ELISA) displayed 100% positivity with the L. (L.) chagasi ESA-blot recognizing bands of molecular weight ranging from 26.5 to 31.5 kDa. The analysis of 160 non-visceral cases showed that 5% of the samples cross-reacted with the L. (L.) chagasi ESA-ELISA and 9.4% reacted with the ESA isolated from L. (L.) amazonensis and L. (V.) braziliensis, whereas a high cross-reaction ranging from 24.4% to 25% was observed with total crude promastigote antigens (PRO-ELISA). The ESA-blot of L. (L.) chagasi tested with non-visceral sera samples showed a cross-reaction with 8.8% of cases; most of these cases represented tegumentary leishmaniasis and only one acute chagasic case. These data lead us to recommend the use of ESA as an alternative antigen in VL diagnosis.
authors: Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, Brazil. vivicancino@usp.br
authors: Viviana
authors: V
authors: Pinedo-Cancino
authors: Norival
authors: N
authors: Kesper
authors: Clara Lúcia
authors: CL
authors: Barbiéri
authors: José Angelo Lauletta
authors: JA
authors: Lindoso
authors: Eufrosina Setsu
authors: ES
authors: Umezawa
mesh_terms: D000953:Antigens, Protozoan / Q000235:genetics / Q000378:metabolism*; D001782:Blood Donors; D001938:Brazil / Q000453:epidemiology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation; D006801:Humans; D015151:Immunoblotting; D007891:Leishmania / Q000145:classification* / Q000378:metabolism*; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology; D007898:Leishmaniasis, Visceral / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology*; D013045:Species Specificity
chemical_list: D000953:Antigens, Protozoan