title: [Expression of HIF-1a in acute limb ischemia of rats].
abstract: OBJECTIVE
  To explore the expression of hypoxia-inducible factor 1 alpha (HIF-1a) in rat lower limb skeletal muscle with acute ischemia.
  
  
  METHODS
  Acute lower limb ischemia was established in 25 male or female SD rats of 200-250 g by ligating left iliac artery with microsurgical techniques. Gastrocnemius of lower limb was harvest at the time points of sham (0h), 4, 8, 12 and 24 h respectively. Immunohistochemistry, Western blot and real-time polymerase chain reaction (PCR) were used to detect the expression of HIF-1a in lower limb gastrocnemius.
  
  
  RESULTS
  There was a low expression of HIF-1a in normal gastrocnemius, but it was highly expressed in lower limb ischemia. With the elongation of ischemia time, its expression increased (P < 0.05), peaked at 4 h and 8 h (P < 0.05) and then declined gradually.
  
  
  CONCLUSIONS
  With a characteristic trend during acute limb ischemia, HIF-1a may play an important role in the adaptive response to acute limb ischemia.
authors: Department of Vascular Surgery, First Affiliated Hospital, China Medical University, Shenyang 110001, China.
authors: Xi
authors: X
authors: Li
authors: Shi-jie
authors: SJ
authors: Xin
authors: Dong
authors: D
authors: Yang
authors: Kui
authors: K
authors: Ding
authors: Ying-hou
authors: YH
authors: Wang
authors: Hui
authors: H
authors: Cao
authors: Jian
authors: J
authors: Zhang
authors: Zhi-quan
authors: ZQ
authors: Duan
mesh_terms: D000818:Animals; D005260:Female; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D007511:Ischemia / Q000378:metabolism*; D035002:Lower Extremity / Q000098:blood supply; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C497444:Hif1a protein, rat; D051795:Hypoxia-Inducible Factor 1, alpha Subunit