title: Magnetic nanoparticles of nitrogen enriched carbon (mnNEC) for analysis of pesticides and metabolites in zebrafish by gas chromatography-mass spectrometry.
abstract: Nanosized carbon based sorbents have been widely used for separation, enrichment and desalting of biological samples because of their distinguished characteristics. In this work, magnetic nanoparticles of nitrogen enriched carbon (mnNEC) have been developed for enrichment of organochlorine pesticide DDT and metabolite DDE that have been accumulated in zebrafish during the course of environmental exposure. Polymerization of pyrrole was performed in the aqueous suspension of Fe(3)O(4) nanoparticles. Resultant core-shell nanoparticules coated with polypyrrole were then subjected to a process of carbonization under high temperature and nitrogen atmospheric condition. The presence of nitrogen atoms in carbon nanoparticles increases the hydrophilicity and dispersability in aqueous samples. It has been experimentally demonstrated that mnNEC can be effectively dispersed in aqueous samples and rapidly isolated by the application of an external magnetic field. Recoveries of DDT and DDE from water range from 90% to 102% and 85-97% respectively. In combination with Selected Ion Monitoring (SIM) experiments of gas chromatography-mass spectrometry, the detection limit can be down to low ng/mL level. By using mnNEC approach, two interesting results have been found for zebrafish with 60 days exposure to DDT (1 μg/l). (1) There is higher concentration of DDT (37-143 ng/g) and DDE (173-1108 ng/g) in male zebrafish body tissues than that of female (7-52 ng/g and 146-362 ng/g for DDT and DDE respectively). (2) There is high ratio of DDE/DDT for both female and male zebrafish, implying high environmental persistence and ongoing bioaccumulation.
authors: Key Laboratory of Pesticides and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, Hubei 430079, PR China.
authors: Youe
authors: Y
authors: Zhou
authors: Qian
authors: Q
authors: Xia
authors: Mengjie
authors: M
authors: Ding
authors: Hubert
authors: H
authors: Kageruka
authors: Haiying
authors: H
authors: Jiang
authors: Yan
authors: Y
authors: Jiang
authors: Huifen
authors: H
authors: Jing
authors: Xiao
authors: X
authors: Xiao
authors: Hongying
authors: H
authors: Zhong
mesh_terms: D000818:Animals; D002244:Carbon / Q000737:chemistry; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008297:Male; D009584:Nitrogen / Q000737:chemistry; D010575:Pesticides / Q000032:analysis* / Q000302:isolation & purification; D011108:Polymers / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000302:isolation & purification; D015027:Zebrafish
chemical_list: D058185:Magnetite Nanoparticles; D010575:Pesticides; D011108:Polymers; D011758:Pyrroles; D014874:Water Pollutants, Chemical; C067635:polypyrrole; D002244:Carbon; D009584:Nitrogen