title: Clinical relevance of plasma miR-106b levels in patients with chronic             obstructive pulmonary disease.
abstract: Chronic obstructive pulmonary disease (COPD) is characterized by both chronic             inflammation in the airway and systemic inflammation; however, the molecular mechanism             of COPD has not been fully elucidated. By measuring microRNA (miRNA) expression             in the plasma of COPD subjects, we aimed to identify the clinical relevance of             plasma miRNA levels in these patients. Blood samples were obtained from COPD patients             and age-matched normal controls. We initially produced plasma miRNA expression             profiles using TaqMan low-density array screening. For further validation, individual             qRT-PCRs were performed in 40 COPD patients and 20 healthy subjects. TaqMan low-density             array screening showed that 9 miRNAs (miR-29b, miR-483-5p, miR-152, miR-629, miR-26b,             miR-101, miR-106b, miR-532-5p and miR-133b) were significantly downregulated in             the plasma from COPD patients when compared with normal smokers. Among these miRNAs,             we focused on miR-106b. A reduction in the plasma miR-106b levels was evident             in COPD ex-smokers and COPD current smokers compared with levels in smokers. There             was a negative correlation between the plasma miR-106b level and the duration             of disease since diagnosis in COPD ex-smokers and the duration of smoking in COPD             current smokers. These findings support the concept that progressive reduction             in the plasma miR-106b level may reflect persistent and systemic changes even             after the discontinuation of smoking in COPD patients. miR-106b may therefore             play an important role in the pathogenesis of COPD.
authors: Division of Respiratory Medicine, Tokyo Medical University, Tokyo, Japan. skume@tokyo-med.ac.jp
authors: Seiko
authors: S
authors: Soeda
authors: Junko H
authors: JH
authors: Ohyashiki
authors: Kazushige
authors: K
authors: Ohtsuki
authors: Tomohiro
authors: T
authors: Umezu
authors: Yasuhiro
authors: Y
authors: Setoguchi
authors: Kazuma
authors: K
authors: Ohyashiki
mesh_terms: D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D012372:ROC Curve; D012907:Smoking / Q000097:blood*; D016212:Transforming Growth Factor beta / Q000097:blood
chemical_list: C542541:MIRN106 microRNA, human; D035683:MicroRNAs; D016212:Transforming Growth Factor beta