title: Phototherapy and resistance training prevent sarcopenia in ovariectomized rats.
abstract: The aim of this study was to histologically and biochemically analyze the effects of light-emitting diode therapy (LEDT) associated with resistance training to prevent sarcopenia in ovariectomized rats. Forty female Wistar rats (12 months old, 295-330 g) were bilaterally ovariectomized and divided into four groups (n = 10 per group): control-sedentary (C), resistance training (T), LEDT-sedentary (L), and LEDT plus resistance training (LT). Trained rats performed a 12-week water-jumping program (3 days per week) carrying a load equivalent to 50-80% of their body mass strapped to their back. Depending on the group protocol, the LED device (850 nm, 100 mW, 120 J/cm(2), spot size 0.5 cm(2)) was used either as the only method or after the resistance training had been performed. The device was used in the single point contact mode (for 10 min). The irradiated region was the center of the greater trochanter of the right femur and the middle third of the rectus femoris muscle was subsequently analyzed histomorphometrically. Significant increases (p < 0.05) were noted for the muscle volume of the T (68.1 ± 19.7%), the L (74.1 ± 5.1%), and the LT (68.2 ± 11.5%) groups compared to the C group (60.4 ± 5.5%). There were also significant increases in the concentrations of IGF-1, IL-1, and TNF-α in the muscles of the treated groups (p < 0.05). Animals in the LT group showed a significant increase in IL-6 compared to T, L, and C groups (p < 0.05). These findings suggest that resistance training and LEDT can prevent sarcopenia in ovariectomized rats.
authors: Department of Morphology, Piracicaba Dental School (FOP), University of Campinas (UNICAMP), Av. Limeira, 901, 13414-903, Piracicaba, SP, Brazil, avcorazza@gmail.com.
authors: Adalberto Vieira
authors: AV
authors: Corazza
authors: Fernanda Rossi
authors: FR
authors: Paolillo
authors: Francisco Carlos
authors: FC
authors: Groppo
authors: Vanderlei Salvador
authors: VS
authors: Bagnato
authors: Paulo Henrique Ferreira
authors: PH
authors: Caria
mesh_terms: D000818:Animals; D005260:Female; D018836:Inflammation Mediators / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D028022:Low-Level Light Therapy; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology; D010052:Ovariectomy / Q000009:adverse effects; D010789:Phototherapy; D010805:Physical Conditioning, Animal; D051381:Rats; D017208:Rats, Wistar; D055070:Resistance Training; D055948:Sarcopenia / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D018836:Inflammation Mediators; D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D007334:Insulin-Like Growth Factor I