title: Intracellular distribution of the ΔNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies.
abstract: The p73 protein is a member of the p53 family of transcription factors that has two N-terminal isoforms: the TAp73 isoform is reported to have a tumor suppressor function, whereas the ΔNp73 isoform likely has oncogenic potential. The expression of these isoforms and the differences in their intracellular distribution have been described in many cancer types; however, little is known about the p73 isoforms in brain tumors. Our study is focused on the intracellular localization of ΔNp73 in medulloblastoma cell lines. Due to a lack of suitable anti-ΔNp73 antibodies, we developed two new rabbit polyclonal antibodies, ΔNp73-26 and ΔNp73-27, with sufficient specificity, as demonstrated by immunodetection methods using transiently transfected cell lines. Both of these new antibodies were subsequently used for analysis of the ΔNp73 distribution in medulloblastoma cells using immunofluorescence, immunoblotting and immunogold labeling for transmission electron microscopy. We found a nuclear localization of the ΔNp73 isoform in all of the medulloblastoma cell lines included in this study. Furthermore, a non-random accumulation of the ΔNp73 isoform near the cell nuclei was observable in all of these cell lines. By double-labeling with ΔNp73 and golgin-97, we showed the co-localization of the ΔNp73 isoform with the Golgi apparatus. Nevertheless, further detailed analyses of possible interactions of ΔNp73 with the proteins accumulated in the Golgi apparatus should be performed to explain the dynamics of ΔNp73 outside the cell nucleus.
authors: Department of Experimental Biology, School of Science, Masaryk University, Brno, Czech Republic. veselska@sci.muni.cz
authors: Renata
authors: R
authors: Veselska
authors: Jakub
authors: J
authors: Neradil
authors: Marta
authors: M
authors: Nekulova
authors: Lucia
authors: L
authors: Dobrucka
authors: Borivoj
authors: B
authors: Vojtesek
authors: Jaroslav
authors: J
authors: Sterba
authors: Karel
authors: K
authors: Zitterbart
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005260:Female; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D061986:MCF-7 Cells; D008297:Male; D008527:Medulloblastoma / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D009687:Nuclear Proteins / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D011817:Rabbits; D014162:Transfection; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
chemical_list: D000906:Antibodies; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D020033:Protein Isoforms; C000606460:TP73 protein, human; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins; C000606369:delta Np73 protein, human