title: A comparative study of the interaction of two structurally analogous ruthenium complexes with human telomeric G-quadruplex DNA.
abstract: Two new polypyridine ligands and their corresponding ruthenium(II) complexes have been prepared and characterized. The interactions of both complexes with human telomere quadruplex DNA (both the antiparallel basket and the mixed-hybrid G-quadruplex) have been studied by circular dichroism (CD), CD melting, UV-visible (UV-Vis), fluorescent intercalator displacement (FID) assays and molecular docking studies. The results show that both complexes can stabilize G-quadruplexes DNA and two complexes show different binding affinity for different G-quadruplexes DNA. The 1:1 stoichiometry was confirmed in the buffered solutions by the UV-Vis spectrophotometer using Job's plot method and molecular docking studies. We have also investigated the interaction between the complexes and duplex DNA to gain some insight into the selectivity of the complexes for G-quadruplex structures. FID studies have shown that the complexes have a modest selectivity for G-quadruplex versus duplex DNA.
authors: Department of Chemistry, Tongji University, Shanghai 200092, PR China. shishuo@tongji.edu.cn
authors: Shuo
authors: S
authors: Shi
authors: Hai-Liang
authors: HL
authors: Huang
authors: Xing
authors: X
authors: Gao
authors: Jun-Liang
authors: JL
authors: Yao
authors: Chun-Yan
authors: CY
authors: Lv
authors: Juan
authors: J
authors: Zhao
authors: Wen-Liang
authors: WL
authors: Sun
authors: Tian-Ming
authors: TM
authors: Yao
authors: Liang-Nian
authors: LN
authors: Ji
mesh_terms: D002942:Circular Dichroism; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D054856:G-Quadruplexes; D006801:Humans; D007364:Intercalating Agents / Q000737:chemistry; D007700:Kinetics; D008024:Ligands; D062105:Molecular Docking Simulation; D015394:Molecular Structure; D011725:Pyridines / Q000138:chemical synthesis / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D016615:Telomere / Q000737:chemistry*
chemical_list: D056831:Coordination Complexes; D007364:Intercalating Agents; D008024:Ligands; D011725:Pyridines; D012428:Ruthenium; D004247:DNA