title: Resveratrol facilitates pain attenuation in a rat model of neuropathic pain through the activation of spinal Sirt1.
abstract: BACKGROUND
  Little research has been conducted regarding the implications of Sirt1 (a classic III HDAC) in neuropathic pain. The aim of this study was to investigate the variation in the expressions of spinal Sirt1 and acetyl-histone H3 in a rat model of chronic constriction injury.
  
  
  METHODS
  A neuropathic pain model of chronic constriction injury (CCI) was established in a unilateral hind limb in Sprague-Dawley rats.
  
  
  RESULTS
  Western blot analysis and immunohistochemistry revealed that Sirt1 (silent information regulator) expression decreased, whereas acetyl-histone H3 increased in the spinal cord following CCI surgery. The intrathecal administration of resveratrol, an activator of Sirt1, attenuated CCI-induced mechanical allodynia and thermal hyperalgesia, increased Sirt1 expression, and decreased acetyl-histone H3 in the spine. Resveratrol induced no obvious histopathological changes in the spinal cord.
  
  
  CONCLUSIONS
  Our data provide new evidence for the contribution of spinal Sirt1 to the initiation and maintenance of neuropathic pain. The antinociceptive effects of resveratrol may be mediated through the activation of spinal Sirt1 in CCI rats.
authors: Xuzhou Medical College, Jiangsu Province Key Laboratory of Anesthesiology and Center for Pain Research and Treatment, and Affiliated Hospital of Xuzhou Medical College, Xuzhou, China.
authors: Qin
authors: Q
authors: Yin
authors: Fei-Fei
authors: FF
authors: Lu
authors: Yu
authors: Y
authors: Zhao
authors: Ming-Yue
authors: MY
authors: Cheng
authors: Qin
authors: Q
authors: Fan
authors: Jie
authors: J
authors: Cui
authors: Lei
authors: L
authors: Liu
authors: Wei
authors: W
authors: Cheng
authors: Chang-Dong
authors: CD
authors: Yan
mesh_terms: D000107:Acetylation; D000700:Analgesics / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D020536:Enzyme Activators / Q000008:administration & dosage / Q000494:pharmacology*; D006657:Histones / Q000378:metabolism; D006930:Hyperalgesia / Q000175:diagnosis / Q000201:enzymology / Q000503:physiopathology / Q000517:prevention & control*; D007150:Immunohistochemistry; D060186:Infusions, Spinal; D008297:Male; D009437:Neuralgia / Q000175:diagnosis / Q000201:enzymology / Q000503:physiopathology / Q000517:prevention & control*; D010147:Pain Measurement; D058748:Pain Perception / Q000187:drug effects; D017288:Pain Threshold / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D056564:Sirtuin 1 / Q000378:metabolism*; D013116:Spinal Cord / Q000187:drug effects* / Q000201:enzymology / Q000503:physiopathology; D013267:Stilbenes / Q000008:administration & dosage / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D000700:Analgesics; D020536:Enzyme Activators; D006657:Histones; D013267:Stilbenes; C488368:Sirt1 protein, rat; D056564:Sirtuin 1; D000077185:Resveratrol