title: Graphene as excellent support for rapid and efficient near infrared-assisted tryptic proteolysis.
abstract: A rapid and efficient tryptic proteolysis approach has been developed by combining graphene with near infrared (NIR)-assisted protein digestion. Based on the unique properties of graphene such as strong absorption ability of electromagnetic radiation over a wide range of wavelengths and excellent thermal conductivity, we developed a novel fast NIR-assisted tryptic digestion method using graphene, allowing the entire time for identification of protein to be greatly reduced. Bovine serum albumin (BSA), myoglobin (Myo), lysozyme (Lys) and cytochrome c (Cyt-c) were denatured with a digestion time as short as 3s. Peptide fragments were identified by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Equivalent or better digestion efficiency was observed comparing to current in-solution digestion methods. This novel graphene-based NIR-assisted digestion approach may provide a promising proteomic identification technique to biomedical and clinical research.
authors: Department of Materials Science and Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.
authors: Chia-Feng
authors: CF
authors: Chang
authors: Quang Duc
authors: QD
authors: Truong
authors: Jiann-Ruey
authors: JR
authors: Chen
mesh_terms: D000818:Animals; D002417:Cattle; D045304:Cytochromes c / Q000737:chemistry* / Q000378:metabolism; D006108:Graphite / Q000737:chemistry*; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D009211:Myoglobin / Q000737:chemistry*; D010446:Peptide Fragments / Q000032:analysis*; D059748:Proteolysis; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D019265:Spectroscopy, Near-Infrared
chemical_list: D009211:Myoglobin; D010446:Peptide Fragments; D012710:Serum Albumin, Bovine; D006108:Graphite; D045304:Cytochromes c; D009113:Muramidase