title: Turn-on fluorescence detection of apoptotic cells using a zinc(II)-dipicolylamine-functionalized poly(diacetylene) liposome.
abstract: A liposome-based fluorescence sensing system for apoptotic cells has been developed from stimuli-responsive poly(diacetylene)-liposomes for the first time. The combination of the liposome components, a phosphatidylserine-binding Zn(II)-dipicolylamine component and an alcohol-terminated component in the ratio of 2:1, has led to an efficient detection system for apoptotic cells, as demonstrated by confocal fluorescence microscopy and FACS analysis. The liposome shows a color change from blue to reddish purple and emits fluorescence in the turn-on mode upon interaction with phosphatidylserine. The present system thus avoids the washing steps required for "always-on"-type sensing systems.
authors: Department of Chemistry and Center for Electro-Photo Behaviors in Advanced Molecular Systems, Pohang University of Science and Technology, San 31 Hyoja-dong, Pohang 790-784, Korea.
authors: Yong-Suk
authors: YS
authors: Cho
authors: Kyung Mi
authors: KM
authors: Kim
authors: Duhwan
authors: D
authors: Lee
authors: Won Jong
authors: WJ
authors: Kim
authors: Kyo Han
authors: KH
authors: Ahn
mesh_terms: D000588:Amines / Q000737:chemistry*; D017209:Apoptosis; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D018613:Microscopy, Confocal; D010718:Phosphatidylserines / Q000737:chemistry; D010848:Picolinic Acids / Q000737:chemistry*; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry*
chemical_list: C501847:2,2'-dipicolylamine; D000588:Amines; D005456:Fluorescent Dyes; D008081:Liposomes; D010718:Phosphatidylserines; D010848:Picolinic Acids; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene; D015032:Zinc