title: A BODIPY-derived fluorescent probe for cellular pH measurements.
abstract: In this study, BODIPY-appended calix[4]arene was chosen as a fluorescent probe for intracellular pH. The compound with cell permeability can monitor the minor pH change near neutrality inside the cell and is the first BODIPY-derived probe reported for cytosolic pH. Owing to a high level of cell retention and minor cytotoxicity of the probe, stable fluorescence is provided in the cells for 24h, facilitating the precise observation of intracellular pH. A model of cell apoptosis was designed by exposure of the cells to a low concentration of hydrogen peroxide. An increase in the fluorescence of the cells confirmed that BODIPY-appended calix[4]arene sensed the fluctuation of the cellular pH during early cell apoptosis. The developed fluorescent pH probe will be useful for the study of cell apoptosis.
authors: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China.
authors: Fangfei
authors: F
authors: Han
authors: Yanmei
authors: Y
authors: Xu
authors: Dechen
authors: D
authors: Jiang
authors: Yu
authors: Y
authors: Qin
authors: Hongyuan
authors: H
authors: Chen
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001896:Boron Compounds / Q000737:chemistry*; D047250:Calixarenes / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D006863:Hydrogen-Ion Concentration; D008856:Microscopy, Fluorescence; D010636:Phenols / Q000737:chemistry
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D010636:Phenols; C121325:calix(4)arene; D047250:Calixarenes; D006861:Hydrogen Peroxide