title: Antimicrobial susceptibilities of Clostridium difficile isolated in Japan.
abstract: Clostridium difficile is a common causative organism of antimicrobial-associated diarrhea and is often responsible for nosocomial infection. C. difficile infection has traditionally been treated with metronidazole (MNZ) or vancomycin (VCM); however, MNZ-resistant strains have reported in some countries. In this study the broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) of 15 drugs against 157 clinical isolates of C. difficile in Japan. All C. difficile isolates tested were susceptible to MNZ; MIC50 was 0.25 μg/ml, MIC90 was 0.5 μg/ml, and MIC range was 0.06-1 μg/ml. C. difficile isolates were also susceptible to VCM; MIC50 was 0.5 μg/ml, MIC90 was 1 μg/ml, and MIC range was 0.12-2 μg/ml. Susceptibility to teicoplanin was also similar: MIC50 was 0.12 μg/ml, MIC90 was 0.12 μg/ml, and MIC range was 0.03-0.25 μg/ml. The susceptibility of C. difficile isolates must be monitored continuously because a strain with reduced susceptibility to MNZ or VCM might emerge in the future.
authors: Department of Infection Control and Laboratory Diagnostics, Internal Medicine, Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai, Japan. h2kuni@med.tohoku.ac.jp
authors: Hiroyuki
authors: H
authors: Kunishima
authors: Junichi
authors: J
authors: Chiba
authors: Michiko
authors: M
authors: Saito
authors: Yoshihiro
authors: Y
authors: Honda
authors: Mitsuo
authors: M
authors: Kaku
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016360:Clostridioides difficile / Q000187:drug effects*; D003015:Clostridium Infections / Q000382:microbiology*; D024881:Drug Resistance, Bacterial; D006801:Humans; D007564:Japan; D008795:Metronidazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D008795:Metronidazole; D014640:Vancomycin