title: Immunosurveillance against tetraploidization-induced colon tumorigenesis.
abstract: Circumstantial evidence suggests that colon carcinogenesis can ensue the transient tetraploidization of (pre-)malignant cells. In line with this notion, the tumor suppressors APC and TP53, both of which are frequently inactivated in colon cancer, inhibit tetraploidization in vitro and in vivo. Here, we show that-contrarily to their wild-type counterparts- Tp53 (-/-)  colonocytes are susceptible to drug-induced or spontaneous tetraploidization in vitro. Colon organoids generated from tetraploid Tp53 (-/-)  cells exhibit a close-to-normal morphology as compared to their diploid Tp53 (-/-)  counterparts, yet the colonocytes constituting these organoids are characterized by an increased cell size and an elevated expression of the immunostimulatory protein calreticulin on the cell surface. The subcutaneous injection of tetraploid Tp53 (-/-)  colon organoids led to the generation of proliferating tumors in immunodeficient, but not immunocompetent, mice. Thus, tetraploid Tp53 (-/-)  colonocytes fail to survive in immunocompetent mice and develop neoplastic lesions in immunocompromised settings only. These results suggest that tetraploidy is particularly oncogenic in the context of deficient immunosurveillance.
authors: INSERM, U848, Villejuif, France.
authors: Alice
authors: A
authors: Boilève
authors: Laura
authors: L
authors: Senovilla
authors: Ilio
authors: I
authors: Vitale
authors: Delphine
authors: D
authors: Lissa
authors: Isabelle
authors: I
authors: Martins
authors: Didier
authors: D
authors: Métivier
authors: Stieneke
authors: S
authors: van den Brink
authors: Hans
authors: H
authors: Clevers
authors: Lorenzo
authors: L
authors: Galluzzi
authors: Maria
authors: M
authors: Castedo
authors: Guido
authors: G
authors: Kroemer
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D037282:Calreticulin / Q000096:biosynthesis; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism*; D003106:Colon / Q000378:metabolism / Q000473:pathology; D003110:Colonic Neoplasms / Q000235:genetics / Q000276:immunology*; D007157:Immunologic Surveillance / Q000276:immunology*; D008565:Membrane Proteins / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D015739:Nocodazole / Q000494:pharmacology; D057891:Tetraploidy; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D037282:Calreticulin; D008565:Membrane Proteins; D016159:Tumor Suppressor Protein p53; D015739:Nocodazole
keywords: apoptosis; cell cycle; cytochalasin D; mitotic catastrophe; nocodazole; p53