title: Novel amelogenin-releasing hydrogel for remineralization of enamel artificial caries.
abstract: Recently, the use of recombinant full-length amelogenin protein in combination with fluoride has shown promising results in the formation of densely packed enamel-like structures. In this study, amelogenin (rP172)-releasing hydrogels containing calcium, phosphate, and fluoride were investigated for remineralization efficacy using in vitro early enamel caries models. The hydrogels were applied to artificial caries lesions on extracted human third molars, and the remineralization efficacy was tested in different models: static gel remineralization in the presence of artificial saliva, pH cyclic treatment at pH 5.4 acetic buffer and pH 7.3 gel remineralization, and treatment with multispecies oral biofilms grown in a continuous flowing constant-depth film fermenter. The surface microhardness of remineralized enamel increased significantly when amelogenin was released from hydrogel. No cytotoxicity was observed when periodontal ligament cells were cultured with the mineralized hydrogels.
authors: Department of Comprehensive Dentistry and Biomaterials, School of Dentistry, Louisiana State University Health Sciences Center, New Orleans, LA, USA.
authors: Yuwei
authors: Y
authors: Fan
authors: Zezhang T
authors: ZT
authors: Wen
authors: Sumei
authors: S
authors: Liao
authors: Thomas
authors: T
authors: Lallier
authors: Joseph L
authors: JL
authors: Hagan
authors: Jefferson T
authors: JT
authors: Twomley
authors: Jian-Feng
authors: JF
authors: Zhang
authors: Zhi
authors: Z
authors: Sun
authors: Xiaoming
authors: X
authors: Xu