title: Direct and real-time observation of rotary movement of a DNA nanomechanical device.
abstract: Analogous to the biologically abundant protein-based linear molecular machines that translocate along their target surface, we have recently constructed the DNA-based synthetic molecular motors that effect linear movement or navigate a network of tracks on a DNA origami substrate. However, a DNA-based molecular machine with rotary function, analogous to rotary proteins, is still unexplored. Here, we report the construction of a rotary motor based on the B-Z conformational transition of DNA and the direct and real-time observation of its function within a frame-shaped DNA origami. The motor can be switched off by introducing conditions that stabilize B-DNA, while it can be fueled by adding Z-DNA-promoting high-saline buffer. When MgCl(2) was used as external stimulus, 70% of the motors rotated, while 76% of the stators/controls exhibited no rotation. Such a motor system could be successfully applied to perform multiple actions aimed for our benefit. Moreover, for the first time we have directly observed the B-Z conformational transition of DNA in real-time, which shed light on the fundamental understanding of DNA conformations.
authors: Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
authors: Arivazhagan
authors: A
authors: Rajendran
authors: Masayuki
authors: M
authors: Endo
authors: Kumi
authors: K
authors: Hidaka
authors: Hiroshi
authors: H
authors: Sugiyama
mesh_terms: D004277:DNA, Single-Stranded / Q000737:chemistry*; D015636:Magnesium Chloride / Q000737:chemistry; D009690:Nucleic Acid Conformation; D012399:Rotation; D013997:Time Factors
chemical_list: D004277:DNA, Single-Stranded; D015636:Magnesium Chloride