title: Bilirubin clearance and antioxidant activities of ethanol extract of Phyllanthus amarus root in phenylhydrazine-induced neonatal jaundice in mice.
abstract: The ability of ethanol extract of Phyllanthus amarus root (EEPA) to decrease bilirubin level and oxidative stress in phenylhydrazine-induced neonatal jaundice in mice was investigated. Administration of phenylhydrazine (75 mg/kg b.w.) significantly elevated total and unconjugated serum bilirubin level compared to control mice. EEPA (5, 10, and 20 mg/kg b.w., oral) dose-dependently reduced the bilirubin level. EEPA treatment also upregulated hepatic CAR and CYP3A1, accounting for its ability to facilitate bilirubin clearance. A single dose of EEPA (20 mg/kg b.w.) induced higher level of bilirubin clearance than phototherapy, widely used for treating neonatal jaundice. Furthermore, phenylhydrazine administration significantly increased MDA, protein carbonyl, and total thiol content and lowered the GSH level along with superoxide dismutase and catalase activity in erythrocyte compared to the control group. Single administration of EEPA (20 mg/kg b.w.) significantly reversed the trend. Presence of gallic acid, gentisic acid, and ortho-coumaric acid in EEPA was identified by HPLC analysis. Amongst these, the major phenolic constituent, gallic acid, exhibited significant bilirubin-lowering effect. These results suggested that P. amarus may be beneficial in reducing bilirubin level as well as oxidative stress in neonatal jaundice.
authors: Department of Biochemistry, University of Calcutta, Kolkata, India.
authors: Soumya
authors: S
authors: Maity
authors: Nivedita
authors: N
authors: Nag
authors: Suchandra
authors: S
authors: Chatterjee
authors: Soumyakanti
authors: S
authors: Adhikari
authors: Santasree
authors: S
authors: Mazumder
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001663:Bilirubin / Q000097:blood*; D002374:Catalase / Q000378:metabolism; D000090702:Constitutive Androstane Receptor; D051544:Cytochrome P-450 CYP3A / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D000431:Ethanol; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D007567:Jaundice, Neonatal / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D010659:Phenylhydrazines; D029803:Phyllanthus / Q000737:chemistry*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D000090702:Constitutive Androstane Receptor; D010659:Phenylhydrazines; D010936:Plant Extracts; D018160:Receptors, Cytoplasmic and Nuclear; C030299:phenylhydrazine; D000431:Ethanol; D002374:Catalase; C465138:Cyp3a23-3a1 protein, rat; D051544:Cytochrome P-450 CYP3A; D013482:Superoxide Dismutase; D005978:Glutathione; D001663:Bilirubin