title: Functional multiplex reporter assay using tagged Gaussia luciferase.
abstract: We have developed a multiplex reporter system to monitor multiple biological variables in real-time. The secreted Gaussia luciferase was fused to ten different epitope tags (Gluc(tag)), each expressed in different tumor cells. By immunobinding of the tags followed by Gluc(tag) detection, this system allowed the independent and real-time monitoring of mixed cell cultures in vitro and of mixed subcutaneous and intracranial tumor subpopulations in vivo.
authors: Department of Neurosurgery, Cancer Center Amsterdam, VU University Medical Center, Amsterdam, The Netherlands.
authors: Sjoerd
authors: S
authors: van Rijn
authors: Jonas
authors: J
authors: Nilsson
authors: David P
authors: DP
authors: Noske
authors: W Peter
authors: WP
authors: Vandertop
authors: Bakhos A
authors: BA
authors: Tannous
authors: Thomas
authors: T
authors: Würdinger
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D033342:Copepoda / Q000201:enzymology*; D000939:Epitopes / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D017930:Genes, Reporter / Q000235:genetics*; D006801:Humans; D008156:Luciferases / Q000235:genetics* / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D014183:Transplantation, Heterologous; D000097573:Red Fluorescent Protein
chemical_list: D000939:Epitopes; D008156:Luciferases; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein