title: [Analysis of muscone from serum and cerebrospinal fluid of rabbits after intragastric administration of tongqiao huoxue granules].
abstract: OBJECTIVE
  To analyse the muscone from serum and cerebrospinal fluid (CSF) of rabbits after intragastric administration of Tongqiao Huoxue granules (TQHXG) and provide the partial experimental reference on laying down quality standard of preparation of formula and offer new ideas on studying the relation between pharmacodynamics and its effective component of formula.
  
  
  METHODS
  Muscone from TQHXG was qualitative identified by the methods of thin-layer chromatography (TLC) and gas chromatography (GC). Muscone from blood and CSF of rabbits was examined by GC after intragastric administration of TQHXG. Compared with standard reference material of muscone, blank serum and blank CSF, the segmental component absorbed into CSF of rabbits was confirmed.
  
  
  RESULTS
  Spots of TQHXG on the chromatomap showed the same colour at the corresponding locality of the standard reference material. Detected by GC, chromatographic peaks appeared at the retention time of 4.8 minute in the chromatomaps of the serum with TQHXG, the CSF with TQHXG and standard reference material of muscone, while there were no chromatographic peaks in the chromatomaps of the blank serum, the blank CSF and methanol at the same time. The ingredient corresponding the chromatographic peaks at the retention time of 4.8 minute in the chromatomaps of the serum, CSF with TQHXG should be muscone.
  
  
  CONCLUSIONS
  Muscone from TQHXG could penetrate blood brain barrier and could be one index of quality standard of preparation of TQHXG.
authors: Anhui Key Laboratory of Modernized Chinese Material, Anhui University of Traditional Chinese Medicine, Hefei 230031, China. wangnhf_515@yahoo.com.cn
authors: Ning
authors: N
authors: Wang
authors: Guo-Jie
authors: GJ
authors: Chang
authors: Qi-Zhong
authors: QZ
authors: Jin
authors: Qun
authors: Q
authors: He
authors: Jing-Song
authors: JS
authors: Liu
authors: Dai-Yin
authors: DY
authors: Peng
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism; D002849:Chromatography, Gas / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods*; D003516:Cycloparaffins / Q000097:blood* / Q000134:cerebrospinal fluid*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism*; D005260:Female; D008297:Male; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism*; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control; D011817:Rabbits
chemical_list: D003516:Cycloparaffins; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; C031021:muscone