title: Hsp110  expression changes in rat primary myocardial cells exposed to heat stress in  vitro.
abstract: We  investigated and described the kinetics of heat shock protein (Hsp) 110  expression and distribution in rat primary myocardial cells exposed to heat  stress in vitro. After incubation at 37°C for 72 h, myocardial cells  were heat stressed at 42°C for 0, 10, 20, 40, 60, 120, 240, 360, and 480 min.  Significant increases in aspartate transaminase, lactate dehydrogenase, and  creatine kinase enzymatic activities in the myocardial cell culture media were  observed during heat stress, suggesting that the integrity of the myocardial  cells was altered. Immunocytochemical analysis revealed that the expressed  Hsp110 was constitutively localized in the cytoplasm and in the nuclei in small  amounts characterized by a granular pattern. Nuclear Hsp110 levels increased  significantly after 240 min of heat stress compared with levels in the control.  The overall levels of Hsp110 expression increased significantly after 20 min.  After 240 min, Hsp110 levels were approximately 1.2-fold higher than those in  the control. Increasing levels of hsp110 messenger RNA detected using  real-time quantitative polymerase chain reaction were observed after 20 min of  heat stress, and the levels peaked with a 10-fold increase after 240 min of  heat stress. These results indicate that the expression of Hsp110 in primary  myocardial cells in vitro is sensitive to hyperthermic stress and that  Hsp110 is involved in the potential acquisition of thermotolerance after heat  stress. Therefore, Hsp110 might play a fundamental role in opposing and  alleviating heat-induced damage caused by hyperthermic stress in primary  myocardial cells.
authors: Animal Pathology Laboratory, Department of Basic Veterinary, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors: Z J
authors: ZJ
authors: Liu
authors: Y J
authors: YJ
authors: Lv
authors: M
authors: M
authors: Zhang
authors: Z H
authors: ZH
authors: Yue
authors: S
authors: S
authors: Tang
authors: A
authors: A
authors: Islam
authors: B
authors: B
authors: Rehana
authors: E D
authors: ED
authors: Bao
authors: J
authors: J
authors: Hartung
mesh_terms: D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002478:Cells, Cultured; D003402:Creatine Kinase / Q000378:metabolism; D017077:Culture Media, Conditioned; D015870:Gene Expression; D005786:Gene Expression Regulation; D050957:HSP110 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D018869:Heat-Shock Response; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000378:metabolism*; D061251:Primary Cell Culture; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats
chemical_list: D017077:Culture Media, Conditioned; D050957:HSP110 Heat-Shock Proteins; C494655:Hspa4 protein, rat; D012333:RNA, Messenger; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D003402:Creatine Kinase