title: Purification, preparation, and use of chaperone-peptide complexes for tumor immunotherapy.
abstract: The molecular chaperone heat-shock protein 70 (Hsp70) possesses immune stimulatory properties that have been employed in the preparation of anticancer vaccines. Hsp70 binds antigenic peptides in the cytoplasm of cancer cells. Hsp70 thus serves as a convenient, non-discriminating transporter of antigens and can protect the peptides during internalization by APC and cross presentation to T lymphocytes. We describe a method for purifying Hsp70-peptide complexes that can be used to prepare molecular chaperone-based vaccines, involving sequential gel filtration, ion exchange, and affinity chromatography.
authors: Molecular and Cellular Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
authors: Ayesha
authors: A
authors: Murshid
authors: Boston University Medical School, Boston, MA, USA.
authors: Jianlin
authors: J
authors: Gong
authors: Molecular and Cellular Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA. scalderw@bidmc.harvard.edu.
authors: Stuart K
authors: SK
authors: Calderwood
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D045744:Cell Line, Tumor; D005591:Chemical Fractionation / Q000379:methods*; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003600:Cytosol / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D051379:Mice; D009369:Neoplasms / Q000628:therapy*; D010446:Peptide Fragments / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism*; D021381:Protein Transport; D014612:Vaccines / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D018840:HSP70 Heat-Shock Proteins; D010446:Peptide Fragments; D014612:Vaccines