title: Versatile and nondestructive photochemical process for biomolecule immobilization.
abstract: Covalent immobilization of unmodified biological materials as proteins has been performed through a one-step and soft method. This process is based on a polyazidophenylene layer derived from the electroreduction of the parent salt 4-azidobenzenediazonium tetrafluoborate on gold substrates. The wavelength used (365 nm) for the photochemical grafting of a large variety of molecules as biomolecules is a key point to this nondestructive immobilization method. This simple process is also versatile and could be used for covalently binding a wide range of molecules such as polyethylene glycol moieties, for example. To validate this approach for biochip or microarray fabrication, a surface plasmon resonance imaging (SPRi) platform for immobilization of various antibody families was created by grafting G-protein through this process. This SPRi antibodies platform was tested with several consecutive cycles of antigen injections/regeneration steps without loss of activity.
authors: CEA, IRAMIS, SPCSI Chemistry of Surfaces and Interfaces Group, F-91191 Gif-sur-Yvette, France.
authors: Pascal
authors: P
authors: Viel
authors: Justine
authors: J
authors: Walter
authors: Sophie
authors: S
authors: Bellon
authors: Thomas
authors: T
authors: Berthelot
mesh_terms: D001386:Azides / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D055767:Immobilized Proteins / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D055668:Photochemical Processes; D020349:Surface Plasmon Resonance; D013499:Surface Properties
chemical_list: D001386:Azides; D055767:Immobilized Proteins; C014747:phenylazide; D006046:Gold