title: Characterization of endocrine disruptors from a complex matrix using estrogen receptor affinity columns and high performance liquid chromatography-high resolution mass spectrometry.
abstract: Complex mixtures of contaminants with potential adverse effects on human health and wildlife are found in the environment and in the food chain. These mixtures include numerous anthropogenic compounds of various origins and structures, which may behave as endocrine disruptors. Mixture's complexity is further enhanced by biotic and abiotic transformations. It is therefore necessary to develop new strategies allowing the identification of the structure of known, as well as unknown, nuclear receptor (NR) ligands present in complex matrices. We explored the possibility to use NR-based affinity columns to characterize the presence of bioactive molecules in environmental complex mixtures. Estrogen receptor α (ERα)-based affinity columns were used to trap and purify estrogenic substances present in surface sediment samples collected in a French river under mixed anthropogenic pressure. We combined biological, biochemical and analytical approaches to characterize the structure of ligands retained on columns and demonstrate the presence of known active molecules such as bisphenol A and octylphenol, but also of unexpected ERα ligands (n-butylparaben, hydroxyl-methyl-benzofuranone). High resolution mass spectrometry results demonstrate that ERα affinity columns can be used for the isolation, purification and identification of known as well as unknown estrogenic contaminants present in complex matrices.
authors: INRA, UMR 1331 TOXALIM (Research Center in Food Toxicology), Xenobiotic Metabolism Team, 180 Chemin de Tournefeuille, 31027 Toulouse, France.
authors: Adeline
authors: A
authors: Jondeau-Cabaton
authors: Amélie
authors: A
authors: Soucasse
authors: Emilien L
authors: EL
authors: Jamin
authors: Nicolas
authors: N
authors: Creusot
authors: Marina
authors: M
authors: Grimaldi
authors: Isabelle
authors: I
authors: Jouanin
authors: Sélim
authors: S
authors: Aït-Aïssa
authors: Patrick
authors: P
authors: Balaguer
authors: Laurent
authors: L
authors: Debrauwer
authors: Daniel
authors: D
authors: Zalko
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D052244:Endocrine Disruptors / Q000032:analysis* / Q000737:chemistry; D004784:Environmental Monitoring / Q000379:methods*; D047628:Estrogen Receptor alpha / Q000737:chemistry*; D019015:Geologic Sediments / Q000032:analysis*; D006801:Humans; D008024:Ligands; D061986:MCF-7 Cells; D013058:Mass Spectrometry / Q000379:methods; D014162:Transfection; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry
chemical_list: C506487:ESR1 protein, human; D052244:Endocrine Disruptors; D047628:Estrogen Receptor alpha; D008024:Ligands; D014874:Water Pollutants, Chemical