title: Effects of ovarian endometriotic fluid exposure on fertilization rate of mouse oocytes and subsequent embryo development.
abstract: BACKGROUND
  Accidental exposure of oocyte/cumulus complex to endometriotic fluid is not uncommon during oocyte retrieval. Only two studies were available on this subject and they gave conflicting results. In this study, we used a mouse model to evaluate the effect of controlled exposure of oocytes to ovarian endometriotic fluid.
  
  
  METHODS
  Mouse oocytes/cumulus complexes (n = 862) were divided into 4 groups, and were exposed to endometriotic fluid (group 1), pooled sera from subjects without endometrioma (group 2), phosphate-buffered saline (group 3), and fertilization medium (controls). After five minutes, oocytes were washed and inseminated. Embryo development was observed daily. The quality of hatching blastocysts was assessed by counting the number of inner cell mass (ICM) and trophectoderm (TE) cells.
  
  
  RESULTS
  The fertilization, cleavage and blastocyst formation rates in the four groups were not statistically different. The proportions of hatching/hatched blastocysts from fertilized oocytes in groups 1 and 2 were significantly lower than those in group 3 and controls (P = 0.015). Hatching blastocysts from all groups showed no significant difference in the number of ICM and TE cells.
  
  
  CONCLUSIONS
  Exposure of mouse oocytes/cumulus complexes to endometriotic fluid had subtle detrimental effects on subsequent blastocyst development. However, one should be cautious in projecting the results of this study to contaminated human oocytes in a clinical setting.
authors: Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors: Waraporn
authors: W
authors: Piromlertamorn
authors: Ubol
authors: U
authors: Saeng-anan
authors: Teraporn
authors: T
authors: Vutyavanich
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology; D001826:Body Fluids / Q000502:physiology*; D002478:Cells, Cultured; D046149:Embryo Culture Techniques; D047108:Embryonic Development / Q000502:physiology*; D004715:Endometriosis / Q000378:metabolism*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D023421:Models, Animal; D009865:Oocytes / Q000502:physiology*; D044967:Serum