title: In vivo validation of quantitative frequency domain  fluorescence tomography.
abstract: We have developed a hybrid frequency domain fluorescence tomography and magnetic resonance imaging system (MRI) for small animal imaging. The main purpose of this system is to obtain quantitatively accurate fluorescence concentration and lifetime images using a multi-modality approach. In vivo experiments are undertaken to evaluate the system. We compare the recovered fluorescence parameters with and without MRI structural a priori information. In addition, we compare two optical background heterogeneity correction methods: Born normalization and utilizing diffuse optical tomography (DOT) functional a priori information. The results show that the concentration and lifetime of a 4.2-mm diameter indocyanine green inclusion located 15 mm deep inside a rat can be recovered with less than a 5% error when functional a priori information from DOT and structural a priori information from MRI are utilized.
authors: University of California, Tu and Yuen Center for Functional Onco-Imaging, Department of Radiological Sciences, 164 Irvine Hall, Irvine, California 92697, USA.
authors: Yuting
authors: Y
authors: Lin
authors: Michael
authors: M
authors: Ghijsen
authors: Orhan
authors: O
authors: Nalcioglu
authors: Gultekin
authors: G
authors: Gulsen
mesh_terms: D000818:Animals; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D008279:Magnetic Resonance Imaging / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D010363:Pattern Recognition, Automated / Q000379:methods*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D041622:Tomography, Optical / Q000295:instrumentation*