title: Flanking sequence determination and event specific detection of transgenic wheat B72-8-11b strain.
abstract: Exogenous fragment sequence and flanking sequence between exogenous fragment and recombinant chromosome of transgenic wheat B72-8-11b were successfully acquired through PCR amplification with cross-matched primers from exogenous genes. Newly acquired exogenous fragment covered the full-length sequence of transformed genes such as transformed plasmid and corresponding functional genes including marker uidA, promoter ubiquitin, lacZ, 1Dx5, and part of sequence of the wheat genome. A specific PCR detection method for transgenic wheat B72-8-11b strain was established on the basis of primers designed according to flanking sequence. The designed primers revealed specific amplification of 132 bp product of transgenic wheat B72-8-11b strain. This method is characteristics of high specificity, high reproducibility, rapid identification, and excellent accuracy for the identification of transgenic wheat B72-8-11b strain.
authors: Liaoning Normal University, No.1 Liushu South Street, Gan Jing Zi District, Dalian 116029, China.
authors: Piqiao
authors: P
authors: Zhang
authors: Junyi
authors: J
authors: Xu
authors: Qiuyue
authors: Q
authors: Zheng
authors: Fengxia
authors: F
authors: Luan
authors: Jijuan
authors: J
authors: Cao
authors: Hesheng
authors: H
authors: Hou
mesh_terms: D016386:Chromosome Walking; D032461:Chromosomes, Plant; D017931:DNA Primers / Q000235:genetics; D005819:Genetic Markers; D018745:Genome, Plant; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*; D014908:Triticum / Q000235:genetics*
chemical_list: D017931:DNA Primers; D005819:Genetic Markers