title: IgD cross-linking induces gene expression profiling changes and enhances apoptosis in chronic lymphocytic leukemia cells.
abstract: Gene profile and functional changes upon IgD cross-linking were evaluated in chronic lymphocytic leukemia (CLL). Microarrays highlighted responsiveness to IgD in all cases, independently of clinico-biological characteristics. Stimulated samples exhibited the down-regulation of transcripts of B-cell receptor signaling and cell-adhesion at 24h and the up-modulation of differentiation and apoptosis genes at 48 h. A significant increase in apoptosis upon ligation was also documented. Furthermore, comparison between IgD and IgM stimulation displayed a differential transcriptional/functional response. In conclusion, CLL respond to IgD displaying expression changes and cell-death enhancement, indicating the apoptosis induction via-IgD as an alternative approach for CLL management.
authors: Division of Hematology, Department of Cellular Biotechnologies and Hematology, Sapienza University, Rome, Italy.
authors: Simona
authors: S
authors: Tavolaro
authors: Nadia
authors: N
authors: Peragine
authors: Sabina
authors: S
authors: Chiaretti
authors: Maria Rosaria
authors: MR
authors: Ricciardi
authors: Sara
authors: S
authors: Raponi
authors: Monica
authors: M
authors: Messina
authors: Simona
authors: S
authors: Santangelo
authors: Marilisa
authors: M
authors: Marinelli
authors: Valeria
authors: V
authors: Di Maio
authors: Francesca Romana
authors: FR
authors: Mauro
authors: Ilaria
authors: I
authors: Del Giudice
authors: Robin
authors: R
authors: Foà
authors: Anna
authors: A
authors: Guarini
mesh_terms: D000368:Aged; D017209:Apoptosis; D002453:Cell Cycle; D045744:Cell Line, Tumor; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007072:Immunoglobulin D / Q000378:metabolism*; D016130:Immunophenotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D007072:Immunoglobulin D