title: Cardioprotective potential of N,α-L-rhamnopyranosyl vincosamide, an indole alkaloid, isolated from the leaves of Moringa oleifera in isoproterenol induced cardiotoxic rats: in vivo and in vitro studies.
abstract: Hitherto unknown protective effect of N,α-L-rhamnopyranosyl vincosamide (VR), isolated from Moringa oleifera leaves in isoproterenol (ISO)-induced cardiac toxicity was evaluated in rats. Oral administration of VR at 40 mg/kg for 7 days markedly reduced the ISO-induced increase in the levels of serum cardiac markers such as troponin-T, creatine kinase-MB, lactate dehydrogenase and glutamate pyruvate transaminase as well as cardiac lipid peroxidation with a parallel increase in the cellular antioxidants suggesting its cardio-protective and free radical scavenging potential, which was latter confirmed by in vitro study. Rats treated with test compound also improved the ISO-induced abnormal changes in ECG as well as in cardiac histology. A reduction in myocardial necrosis was further evidenced by the tri-phenyl tetrazolium chloride (TTC) stain in isolated test drug pretreated rats. These findings suggest the cardio-protective potential of the isolated alkaloid and possibly the beneficial action is mediated through its free radical scavenging property.
authors: School of Life Sciences, Devi Ahilya University, Indore 452017, India. spanda4@rediffmail.com
authors: Sunanda
authors: S
authors: Panda
authors: Anand
authors: A
authors: Kar
authors: Pratibha
authors: P
authors: Sharma
authors: Ashok
authors: A
authors: Sharma
mesh_terms: D000818:Animals; D002316:Cardiotonic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D054715:Cardiotoxins; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006331:Heart Diseases / Q000139:chemically induced / Q000517:prevention & control*; D026121:Indole Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007545:Isoproterenol; D029588:Moringa oleifera / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002316:Cardiotonic Agents; D054715:Cardiotoxins; D016166:Free Radical Scavengers; D026121:Indole Alkaloids; D010936:Plant Extracts; D007545:Isoproterenol