title: Prostate cancer cells metabolize d-lactate inside mitochondria via a D-lactate dehydrogenase which is more active and highly expressed than in normal cells.
abstract: Although D-lactate metabolism has been shown to occur in a variety of mitochondria, the metabolic fate of D-lactate in cancer cells has never been investigated, as it is believed to be exported to the extracellular phase. We show that mitochondria from both cancer (PC-3) and normal (PNT1A) prostate cells can metabolize D-lactate in an energy competent manner. This is due to the mitochondrial D-lactate dehydrogenase, a membrane flavoprotein, the activity and protein level of which are higher in PC-3 than in PNT1A cells, as detected by both kinetic and immunological analysis. D-Lactate can enter prostate mitochondria and cause the export of newly synthesized malate in a carrier-mediated manner, with the rate of malate efflux from mitochondria twofold higher in cancer.
authors: Istituto di Biomembrane e Bioenergetica, Consiglio Nazionale delle Ricerche, Bari, Italy. l.debari@ibbe.cnr.it
authors: Lidia
authors: L
authors: de Bari
authors: Loredana
authors: L
authors: Moro
authors: Salvatore
authors: S
authors: Passarella
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D006801:Humans; D007700:Kinetics; D045022:Lactate Dehydrogenases / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism*; D008297:Male; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000201:enzymology* / Q000378:metabolism / Q000502:physiology; D051336:Mitochondrial Membranes / Q000201:enzymology; D008933:Mitochondrial Swelling; D010084:Oxidation-Reduction; D011467:Prostate / Q000201:enzymology / Q000473:pathology; D011471:Prostatic Neoplasms; D021381:Protein Transport
chemical_list: D019344:Lactic Acid; D045022:Lactate Dehydrogenases; C071195:D-lactate dehydrogenase