title: Generation and characterization of a monoclonal antibody that cross-reacts with the envelope protein from the four dengue virus serotypes.
abstract: Dengue viruses (DENVs; serotypes 1-4) are members of the flavivirus family. The envelope protein (E) of DENV has been defined as the principal antigenic target in terms of protection and diagnosis. Antibodies that can reliably detect the E surface glycoprotein are necessary for describing and mapping new DENV epitopes as well as for developing more reliable and inexpensive diagnostic assays. In this study, we describe the production and characterization of a monoclonal antibody (mAb) against a recombinant DENV-2 E protein that recognizes a sequential antigen in both native and recombinant form located in domain II of the E protein of all four DENV serotypes. We confirmed that this mAb, C21, recognizes a sequence located in the fusion peptide. In addition, C21 does not have neutralizing activity against DENV-2 in an in vitro system. Furthermore, the C21 mAb is an ideal candidate for the development of research reagents for studying DENV biology because it cross-reacts with the four dengue serotypes.
authors: Department of Molecular Biomedicine, CINVESTAV, México City, México D.F.
authors: Moisés
authors: M
authors: León-Juárez
authors: Julio
authors: J
authors: García-Cordero
authors: Leopoldo
authors: L
authors: Santos-Argumedo
authors: Héctor
authors: H
authors: Romero-Ramírez
authors: Jazmín
authors: J
authors: García-Machorro
authors: José
authors: J
authors: Bustos-Arriaga
authors: Benito
authors: B
authors: Gutiérrez-Castañeda
authors: Nicolás V
authors: NV
authors: Sepúlveda
authors: Gabriela
authors: G
authors: Mellado-Sánchez
authors: Leticia
authors: L
authors: Cedillo-Barrón
mesh_terms: D000330:Aedes; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology*; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003429:Cross Reactions; D003716:Dengue Virus / Q000145:classification / Q000235:genetics / Q000276:immunology*; D018604:Epitope Mapping; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D058889:Molecular Typing; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins
keywords: Dengue virus; dengue viruse-envelope protein; monoclonal antibody