title: Up-regulation of proliferative and migratory genes in regulatory T cells from patients with metastatic castration-resistant prostate cancer.
abstract: A higher frequency of regulatory T cells (Tregs) has been observed in peripheral blood mononuclear cells (PBMC) of patients with different types of solid tumors and hematological malignancies as compared to healthy donors. In prostate cancer patients, Tregs in PBMC have been shown to have increased suppressive function. Tumor-induced biological changes in Tregs may enable tumor cells to escape immunosurveillance. We performed genome-wide expression analyses comparing the expression levels of more than 38,500 genes in Tregs with similar suppressive activity, isolated from the peripheral blood of healthy donors and patients with metastatic castration-resistant prostate cancer (mCRPC). The differentially expressed genes in mCRPC Tregs are involved in cell cycle processes, cellular growth and proliferation, immune responses, hematological system development and function and the interleukin-2 (IL-2) and transforming growth factor-β (TGF-β) pathways. Studies revealed that the levels of expression of genes responsible for T-cell proliferation (C-FOS, C-JUN and DUSP1) and cellular migration (RGS1) were greater in Tregs from mCRPC patients as compared to values observed in healthy donors. Increased RGS1 expression in Tregs from mCRPC patients suggests a decrease in these Tregs' migratory ability. In addition, the higher frequency of CD4(+) CD25(high) CD127(-) Tregs in the peripheral blood of mCRPC patients may be the result of an increase in Treg proliferation capacity. Results also suggest that the alterations observed in gene expression profiles of Tregs in mCRPC patients may be part of the mechanism of tumor escape from host immune surveillance.
authors: Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors: Ngar-Yee
authors: NY
authors: Huen
authors: Alan Lap-Yin
authors: AL
authors: Pang
authors: Jo A
authors: JA
authors: Tucker
authors: Tin-Lap
authors: TL
authors: Lee
authors: Matteo
authors: M
authors: Vergati
authors: Caroline
authors: C
authors: Jochems
authors: Chiara
authors: C
authors: Intrivici
authors: Vittore
authors: V
authors: Cereda
authors: Wai-Yee
authors: WY
authors: Chan
authors: Owen M
authors: OM
authors: Rennert
authors: Ravi A
authors: RA
authors: Madan
authors: James L
authors: JL
authors: Gulley
authors: Jeffrey
authors: J
authors: Schlom
authors: Kwong Y
authors: KY
authors: Tsang
mesh_terms: D000328:Adult; D000368:Aged; D002465:Cell Movement; D049109:Cell Proliferation; D017322:Clinical Trials, Phase II as Topic; D054638:Dual Specificity Phosphatase 1 / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D008875:Middle Aged; D015337:Multicenter Studies as Topic; D009362:Neoplasm Metastasis; D011471:Prostatic Neoplasms / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D020710:RGS Proteins / Q000378:metabolism; D016032:Randomized Controlled Trials as Topic; D050378:T-Lymphocytes, Regulatory / Q000166:cytology*; D016212:Transforming Growth Factor beta / Q000378:metabolism; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D007376:Interleukin-2; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D020710:RGS Proteins; C080831:RGS1 protein, human; D016212:Transforming Growth Factor beta; C517019:DUSP1 protein, human; D054638:Dual Specificity Phosphatase 1