title: Electron microscopy of bone.
abstract: Mineralized tissues like bone, dentin and mineralized cartilage are difficult to prepare for ultrastructural analysis. In general, the higher the level of mineralization is, the more difficult it is to obtain ultrathin sections of these tissue. Tissues with a low level of mineral, e.g. from young animals, are rather simple to prepare and sectioning is not that much of a problem. In the present chapter we describe step-by-step how to prepare mineralized tissues for ultrastructural examination.
authors: Department of Oral Cell Biology, Academic Centre of Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Amsterdam, The Netherlands.
authors: V
authors: V
authors: Everts
authors: K A
authors: KA
authors: Hoeben
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000737:chemistry / Q000648:ultrastructure*; D002021:Buffers; D003648:Decalcification Technique; D004853:Epoxy Resins / Q000737:chemistry; D005404:Fixatives / Q000737:chemistry; D006801:Humans; D051379:Mice; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D008867:Microtomy; D016613:Plastic Embedding; D013194:Staining and Labeling; D016707:Tissue Fixation / Q000379:methods
chemical_list: D002021:Buffers; D004853:Epoxy Resins; D005404:Fixatives