title: pH dependence of the fluorescence lifetime of FAD in solution and in cells.
abstract: We have studied physiological parameters in a living cell using fluorescence lifetime imaging of endogenous chromophores. In this study, pH dependence of the fluorescence lifetime of flavin adenine dinucleotide (FAD), that is a significant cofactor exhibiting autofluorescence, has been investigated in buffer solution and in cells. The fluorescence lifetime of FAD remained unchanged with pH 5 to 9 in solution. However, the fluorescence lifetime in HeLa cells was found to decrease with increasing intracellular pH, suggesting that pH in a single cell can be estimated from the fluorescence lifetime imaging of FAD without adding exogenous fluorescent probes.
authors: Graduate School of Environmental Science, Hokkaido University, Sapporo 060-0810, Japan. takan@es.hokudai.ac.jp.
authors: Md Serajul
authors: MS
authors: Islam
authors: Masato
authors: M
authors: Honma
authors: Takakazu
authors: T
authors: Nakabayashi
authors: Masataka
authors: M
authors: Kinjo
authors: Nobuhiro
authors: N
authors: Ohta
mesh_terms: D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry* / Q000378:metabolism; D005453:Fluorescence; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration
chemical_list: D005182:Flavin-Adenine Dinucleotide