title: DNA barcoding via counterstaining with AT/GC sensitive ligands in injection-molded all-polymer nanochannel devices.
abstract: Nanochannel technology, coupled with a suitable DNA labeling chemistry, is a powerful approach for performing high-throughput single-molecule mapping of genomes. Yet so far nanochannel technology has remained inaccessible to the broader research community due to high fabrication cost and/or requirement of specialized facilities/skill-sets. In this article we show that nanochannel-based mapping can be performed in all polymer chips fabricated via injection molding: a fabrication process so inexpensive that the devices can be considered disposable. Fluorescent intensity variations can be obtained from molecules extended in the polymer nanochannels via chemical counterstaining against YOYO-1. In particular, we demonstrate that the counterstaining induced fluorescent intensity variations to a large degree appear to be proportional to the theoretically computed sequence-maps of both local AT and GC variation along DNA sequences.
authors: Department of Micro- and Nanotechnology, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark.
authors: Peter Friis
authors: PF
authors: Østergaard
authors: Marco
authors: M
authors: Matteucci
authors: Walter
authors: W
authors: Reisner
authors: Rafael
authors: R
authors: Taboryski
mesh_terms: D004247:DNA / Q000032:analysis; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D008024:Ligands; D036103:Nanotechnology / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D011687:Purines / Q000032:analysis*; D011743:Pyrimidines / Q000032:analysis*
chemical_list: D008024:Ligands; D011108:Polymers; D011687:Purines; D011743:Pyrimidines; D004247:DNA