title: Antibody functionalization with a dual reactive hydrazide/click crosslinker.
abstract: A water-soluble, dual reactive hydrazide/click crosslinker (ethynyl hydrazide, EH) was synthesized and characterized. A model antibody, human immunoglobulin G (hIgG), was ethynylated by conventional oxidation/hydrazide reactions with the hydrazide moiety of EH. The terminal alkyne conjugated to the glycan of hIgG was easily functionalized by quantitative and bioorthogonal Cu(I)-catalyzed azide-alkyne cycloaddition. The potential of the hydrazide/click crosslinker as a reagent to functionalize antibodies was demonstrated with fluorophore labeling and antibody immobilization.
authors: Graduate School of East-West Medical Science, Kyung Hee University, Gyeonggi-do 449-701, Republic of Korea.
authors: Hoa Thi
authors: HT
authors: Le
authors: Jae-Gyu
authors: JG
authors: Jang
authors: Ji Young
authors: JY
authors: Park
authors: Choon Woo
authors: CW
authors: Lim
authors: Tae Woo
authors: TW
authors: Kim
mesh_terms: D000480:Alkynes / Q000737:chemistry*; D055910:Antibodies, Immobilized / Q000737:chemistry; D001386:Azides / Q000737:chemistry*; D002384:Catalysis; D057930:Click Chemistry; D003300:Copper / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry*; D003500:Cyclization; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D006834:Hydrazines / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry*
chemical_list: D000480:Alkynes; D055910:Antibodies, Immobilized; D001386:Azides; D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D006834:Hydrazines; D007074:Immunoglobulin G; D003300:Copper