title: Cannabinoid CB1 receptor is expressed in chromophobe renal cell carcinoma and renal oncocytoma.
abstract: OBJECTIVE
  To analyze the mRNA and protein expression of cannabinoid receptors CB1 and CB2 in chromophobe renal cell carcinoma (ChRCC) and renal oncocytoma (RO).
  
  
  METHODS
  Fresh and formalin-fixed tissue samples of ChRCC and RO were analyzed by using real-time quantitative RT-PCR and immunohistochemical techniques (n=40).
  
  
  RESULTS
  Quantitative RT-PCR analysis showed that CB1 mRNA was underexpressed by 12-fold in ChRCC and had a variable expression in RO. CB1 protein showed intense positive immunostaining in both neoplasms. Both CB2 mRNA and protein were not expressed in tumor and non tumor renal tissue.
  
  
  CONCLUSIONS
  This distinct immunoprofile may eventually be used as an additional tool with practical interest in the differential diagnosis of renal tumors.
authors: Department of Physiology, Faculty of Medicine and Dentistry, University of the Basque Country UPV/EHU, Leioa, Bizkaia, Spain. gorka.larrinaga@ehu.es
authors: Gorka
authors: G
authors: Larrinaga
authors: Begoña
authors: B
authors: Sanz
authors: Lorena
authors: L
authors: Blanco
authors: Itxaro
authors: I
authors: Perez
authors: María L
authors: ML
authors: Candenas
authors: Francisco M
authors: FM
authors: Pinto
authors: Amaia
authors: A
authors: Irazusta
authors: Javier
authors: J
authors: Gil
authors: José I
authors: JI
authors: López
mesh_terms: D018249:Adenoma, Oxyphilic / Q000378:metabolism*; D002292:Carcinoma, Renal Cell / Q000378:metabolism*; D006801:Humans; D007680:Kidney Neoplasms / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D043884:Receptor, Cannabinoid, CB1 / Q000096:biosynthesis*
chemical_list: D012333:RNA, Messenger; D043884:Receptor, Cannabinoid, CB1