title: Biochemical analysis of naturally processed antigenic peptides presented by MHC class I molecules.
abstract: Immune surveillance of infected or tumor cells by CD8(+) T cells requires that MHC class I molecules present a diverse repertoire of peptides on the cell surface. Even a few copies of individual peptides among this mixture are sufficient for recognition by the antigen receptors of appropriate CD8(+) T cells. Here we describe a method for biochemical analysis of the naturally processed peptides in living cells. The peptide mixture in cell extracts is fractionated using reverse phase high performance liquid chromatography and detected by the activation of CD8(+) T cell hybridomas. The results provide information on the structure and amount of the peptides and yield insights into the mechanisms that generate the naturally processed peptides.
authors: Department of Pathology, Sapporo Medical University, Sapporo, Japan.
authors: Takayuki
authors: T
authors: Kanaseki
authors: Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA. nshastri@berkeley.edu.
authors: Nilabh
authors: N
authors: Shastri
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002470:Cell Survival; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006825:Hybridomas / Q000166:cytology; D051379:Mice; D010455:Peptides / Q000276:immunology* / Q000378:metabolism; D059748:Proteolysis; D014162:Transfection
chemical_list: D015395:Histocompatibility Antigens Class I; D010455:Peptides