title: Gemi: PCR primers prediction from multiple alignments.
abstract: Designing primers and probes for polymerase chain reaction (PCR) is a preliminary and critical step that requires the identification of highly conserved regions in a given set of sequences. This task can be challenging if the targeted sequences display a high level of diversity, as frequently encountered in microbiologic studies. We developed Gemi, an automated, fast, and easy-to-use bioinformatics tool with a user-friendly interface to design primers and probes based on multiple aligned sequences. This tool can be used for the purpose of real-time and conventional PCR and can deal efficiently with large sets of sequences of a large size.
authors: Facultés de Médecine et de Pharmacie, Aix Marseille Université, URMITE, UM 63, CNRS 7278, INSERM, U1095, 13385 Marseille Cedex 05, France.
authors: Haitham
authors: H
authors: Sobhy
authors: Philippe
authors: P
authors: Colson