title: Ultra high performance liquid chromatography-quadrupole-time of flight analysis for the identification and the determination of resveratrol and its metabolites in mouse plasma.
abstract: Resveratrol is a polyphenol that has numerous interesting biological properties, but, per os, it is quickly metabolized. Some of its metabolites are more concentrated than resveratrol, may have greater biological activities, and may act as a kind of store for resveratrol. Thus, to understand the biological impact of resveratrol on a physiological system, it is crucial to simultaneously analyze resveratrol and its metabolites in plasma. This study presents an analytical method based on UHPLC-Q-TOF mass spectrometry for the quantification of resveratrol and of its most common hydrophilic metabolites. The use of (13)C- and D-labeled standards specific to each molecule led to a linear calibration curve on a larger concentration range than described previously. The use of high resolution mass spectrometry in the full scan mode enabled simultaneous identification and quantification of some hydrophilic metabolites not previously described in mice. In addition, UHPLC separation, allowing run times lower than 10 min, can be used in studies that requiring analysis of many samples.
authors: Université Paris Descartes, Sorbonne Paris cité, EA 4463, Faculté des Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, Paris 75270, France. marie-claude.menet@parisdescartes.fr
authors: M C
authors: MC
authors: Menet
authors: C H
authors: CH
authors: Cottart
authors: M
authors: M
authors: Taghi
authors: V
authors: V
authors: Nivet-Antoine
authors: D
authors: D
authors: Dargère
authors: F
authors: F
authors: Vibert
authors: O
authors: O
authors: Laprévote
authors: J-L
authors: JL
authors: Beaudeux
mesh_terms: D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D013058:Mass Spectrometry / Q000191:economics / Q000379:methods*; D051379:Mice; D000077185:Resveratrol; D012680:Sensitivity and Specificity; D013267:Stilbenes / Q000097:blood* / Q000378:metabolism*; D013997:Time Factors
chemical_list: D013267:Stilbenes; D000077185:Resveratrol