title: Monomer-collagen interactions studied by saturation transfer difference NMR.
abstract: Functional monomers in dentin adhesives are involved in wetting dental substrates, demineralization, and the formation of calcium salts. However, the interaction of these monomers with collagen is not understood at a molecular/atomic level. We performed saturation transfer difference (STD) NMR spectroscopy to investigate the binding interaction of 2 functional monomers, 4-methacryloyloxyethyl trimellitate anhydride (4-META) and 10-methacryloyloxydecyl dihydrogenphosphate (MDP), with atelocollagen as a triple-helical peptide model. High STD intensities were detected on the protons in the aliphatic region in MDP, whereas they were not detected for 4-META. The STD results imply that MDP has a relatively stable interaction with the collagen, because of the hydrophobic interactions between the hydrophobic MDP moieties and the hydrophobic collagen surface. This finding indicates that MDP-collagen complexation accounts for stable dentin bonding.
authors: Cariology and Operative Dentistry, Department of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan. hiraope@tmd.ac.jp
authors: N
authors: N
authors: Hiraishi
authors: N
authors: N
authors: Tochio
authors: T
authors: T
authors: Kigawa
authors: M
authors: M
authors: Otsuki
authors: J
authors: J
authors: Tagami
mesh_terms: D003094:Collagen / Q000737:chemistry*; D001840:Dental Bonding; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D018604:Epitope Mapping / Q000379:methods; D057927:Hydrophobic and Hydrophilic Interactions; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008689:Methacrylates / Q000737:chemistry*; D011522:Protons; D019279:Resin Cements / Q000737:chemistry*
chemical_list: D017220:Dentin-Bonding Agents; D008024:Ligands; D008689:Methacrylates; D011522:Protons; D019279:Resin Cements; C059821:atelocollagen; C034139:4-methacryloxyethyltrimellitic acid anhydride; C069749:methacryloyloxydecyl dihydrogen phosphate; D003094:Collagen