title: [Comparison on physicochemical properties and immun activities of polysaccharides from cultures at different development stages of Dendrobium huoshanense].
abstract: OBJECTIVE
  To compare the physicochemical properties and immun activities of polysaccharides extracted from cultures at different development stages of Dendrobium huoshanense.
  
  
  METHODS
  Polysaccharides were extracted by hot water and alcohol precipitation, and isolated by anion exchange chromatography. The physicochemical properties of polysaccharides were analyzed by spectrophotometry, GC-MS, IR and fluorophore-assisted carbohydrate electrophoresis (FACE). ELISA was used to determine the immun activities of polysaccharides.
  
  
  RESULTS
  The polysaccharides from cultures at different development stages of D. huoshanense had no obvious discrepancy in contents. 5 polysaccharides were isolated by anion exchange chromatography with the same elution conditions. GC-MS analysis showed that the polysaccharides from cultures at different development stages of D. huoshanense consisted of glucose, mannose and galactose. IR and FACE displayed that different polysaccharides had no significant change in chemical properties. In vitro experiments with spleen cells proved that the polysaccharides from cultures at different development stages had similar effects on stimulating IFN-gamma and TNF-alpha releases.
  
  
  CONCLUSIONS
  No significant differences are found in the physico-chemical properties and immun activities of polysaccharides extracted from cultures at different development stages of D. huoshanense, suggesting that the polysaccharides of D. huoshanense have stability in quality to some extent during tissue culture.
authors: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China. 412330571@qq.com
authors: Cheng
authors: C
authors: Chen
authors: Hu-Qi
authors: HQ
authors: Wu
authors: Xue-Qiang
authors: XQ
authors: Zha
authors: Li-Hua
authors: LH
authors: Pan
authors: Jian-Ping
authors: JP
authors: Luo
mesh_terms: D000818:Animals; D031667:Dendrobium / Q000737:chemistry* / Q000254:growth & development*; D004797:Enzyme-Linked Immunosorbent Assay; D007371:Interferon-gamma / Q000378:metabolism; D008297:Male; D051379:Mice; D010946:Plants, Medicinal / Q000737:chemistry / Q000254:growth & development; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D027343:Rhizome / Q000737:chemistry / Q000254:growth & development; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D013997:Time Factors; D046509:Tissue Culture Techniques / Q000379:methods; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma