title: Structural determination and antitumor activities of a water-soluble polysaccharide from Mortierella hepiali.
abstract: A water-soluble polysaccharide (MHP-1) isolated from cultured Mortierella hepiali was obtained by hot-water extraction, DEAE-cellulose 52 anion-exchange, and Sephadex G-25 gel permeation chromatography. Its structural characteristics were investigated by PMP pre-column derivation, partial hydrolysis with acid, methylation analysis, as well as FTIR and NMR spectroscopy. Data revealed that MHP-1 possessed a backbone consisting of the repeating unit→2)-β-d-Manp-(1, 6)-β-d-Glcp-(1→and 3)-β-d-Manp-(1→, branching at O-4 of→2)-β-d-Manp-(1, and terminated with β-d-Manp. The antitumor effects of MHP-1 on H22 tumor-bearing mice were studied. Results showed that MHP-1 significantly inhibited the growth of implanted H22 tumors in mice in vivo, but not on H22, A549, and Hep-G2 cells in vitro. Serum and spleen cells were separated to evaluate the concentrations of TNF-α by ELISA kit. The release of TNF-α was markedly induced by MHP-1 in a dose-dependent manner.
authors: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
authors: Ying
authors: Y
authors: Wang
authors: Huan
authors: H
authors: Zhao
authors: Xiaoliang
authors: X
authors: Miao
authors: Dan
authors: D
authors: Liu
authors: Huixing
authors: H
authors: Jiang
authors: Peipei
authors: P
authors: Liu
authors: Yufeng
authors: Y
authors: Wang
authors: Hongping
authors: H
authors: Yin
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015394:Molecular Structure; D020094:Mortierella / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D001688:Biological Products; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha