title: Antigen-coated poly α-hydroxy acid based microparticles for heterologous prime-boost adenovirus based vaccinations.
abstract: Adenoviruses show promising potential as vectors for cancer vaccines, however, their high immunogenicity can be problematic when it comes to homologous prime-boost strategies. In the studies presented here we show that heterologous prime-boost vaccinations involving ovalbumin (OVA)-antigen-coated microparticles as a prime, and adenovirus encoding OVA (AdOVA) as a boost, were equally as effective as homologous AdOVA prime-boosts at generating OVA-specific CD8(+) T-cell responses, which translated into effective tumor protection. OVA-coated biodegradable poly α-hydroxy acid-based microparticles of varying chemistries, when used as primes in heterologous prime-boost vaccinations, were comparable in terms of promoting OVA-specific CD8(+) T cells as well as providing protection against subsequent tumor challenge. These findings auger well for using poly α-hydroxy acid-based microparticles in prime-boost viral vaccination strategies geared toward the safer, and potentially more efficient, generation of anti-tumor immunity.
authors: College of Pharmacy, University of Iowa S228 PHAR, Iowa City, IA 52242, USA.
authors: Caitlin D
authors: CD
authors: Lemke
authors: Sean M
authors: SM
authors: Geary
authors: Vijaya B
authors: VB
authors: Joshi
authors: Aliasger K
authors: AK
authors: Salem
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D000941:Antigens / Q000737:chemistry*; D019496:Cancer Vaccines / Q000737:chemistry* / Q000235:genetics; D045744:Cell Line, Tumor; D005260:Female; D005822:Genetic Vectors; D006880:Hydroxy Acids / Q000737:chemistry*; D008297:Male; D051379:Mice
chemical_list: D000941:Antigens; D019496:Cancer Vaccines; D006880:Hydroxy Acids