title: Thymic extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue: a gene methylation study.
abstract: Although rare, thymic mucosa-associated lymphoid tissue (MALT) lymphoma is considered to be a distinct clinicopathological entity. Using a methylation-specific polymerase chain reaction, we analyzed thymic MALT lymphomas (n = 18) for their methylation of the following seven tumor suppressor genes: DAPK1, p16(INK4A), p14(ARF), CDH1, RARB, TIMP3 and MGMT. Reactive lymph nodes (n = 16) were used as a control. Of the seven genes examined, thymic MALT lymphomas had an increased number of genes that were methylated (2.9 genes) as compared with reactive lymph nodes (0.63, p = 0.0003). In particular, thymic MALT lymphomas showed a frequent methylation of DAPK1, CDH1, TIMP3 and p14(ARF). In addition, gene methylation of p14(ARF) was associated with a larger tumor size, while that of the other three genes was not associated with any clinicopathological features examined. This study suggests that methylation of tumor suppressor genes may play an important role in thymic MALT lymphoma.
authors: Department of Anatomic Pathology and Molecular Diagnostics, Graduate School of Medical Sciences, Nagoya City University , Nagoya , Japan.
authors: Hisashi
authors: H
authors: Takino
authors: Chunmei
authors: C
authors: Li
authors: Seiji
authors: S
authors: Yamada
authors: Fumihiko
authors: F
authors: Sato
authors: Ayako
authors: A
authors: Masaki
authors: Yukio
authors: Y
authors: Fujiyoshi
authors: Hideo
authors: H
authors: Hattori
authors: Hiroshi
authors: H
authors: Inagaki
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD; D015820:Cadherins / Q000235:genetics; D019175:DNA Methylation; D015254:DNA Modification Methylases / Q000235:genetics; D045643:DNA Repair Enzymes / Q000235:genetics; D064550:Death-Associated Protein Kinases / Q000235:genetics; D044127:Epigenesis, Genetic; D005260:Female; D006801:Humans; D018442:Lymphoma, B-Cell, Marginal Zone / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D018168:Receptors, Retinoic Acid / Q000235:genetics; D012189:Retrospective Studies; D013953:Thymus Neoplasms / Q000235:genetics* / Q000473:pathology; D019717:Tissue Inhibitor of Metalloproteinase-3 / Q000235:genetics; D047368:Tumor Burden; D025521:Tumor Suppressor Proteins / Q000235:genetics; D055815:Young Adult
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; D018168:Receptors, Retinoic Acid; D019717:Tissue Inhibitor of Metalloproteinase-3; D025521:Tumor Suppressor Proteins; C082925:retinoic acid receptor beta; D015254:DNA Modification Methylases; C515491:MGMT protein, human; C579096:DAPK1 protein, human; D064550:Death-Associated Protein Kinases; D045643:DNA Repair Enzymes