title: Sequence polymorphism in the Trypanosoma rangeli HSP70 coding genes allows typing of the parasite KP1(+) and KP1(-) groups.
abstract: The genes encoding the Trypanosoma rangeli heat shock protein 70kDa were sequenced and their genomic organization determined. This human parasite has medical relevance as it shares antigens, hosts and geographical regions with the etiological agent of Chagas' disease, Trypanosoma cruzi. The T. rangeli HSP70 genes are highly conserved regarding their tandem organization, and deduced amino acid sequences among T. rangeli KP1(+) and KP1(-) groups and other trypanosomatids. Nevertheless, a variable number of the immunogenic GMPG motif was observed among HSP70 copies within the same T. rangeli isolate and among different isolates. Interestingly, a polymorphism at nucleotide level affecting the SphI restriction site allowed the differentiation of KP1(-) and KP1(+) groups.
authors: Laboratorio de Parasitología Molecular, Departamento de Microbiología, Facultad Ciencias, Pontificia Universidad Javeriana, Carrera 7 No. 43-82, Edificio 50, Laboratorio 113, Bogotá, Colombia. claudia.cuervo@javeriana.edu.co
authors: Claudia
authors: C
authors: Cuervo
authors: M Carmen
authors: MC
authors: Thomas
authors: Manuel C
authors: MC
authors: López
authors: Concepción J
authors: CJ
authors: Puerta
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D016054:DNA, Protozoan / Q000737:chemistry; D016678:Genome; D005838:Genotype; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment; D017421:Sequence Analysis; D057509:Trypanosoma rangeli / Q000145:classification / Q000235:genetics* / Q000378:metabolism
chemical_list: D016054:DNA, Protozoan; D018840:HSP70 Heat-Shock Proteins