title: Lamin A/C is expressed in pluripotent mouse embryonic stem cells.
abstract: The pluripotent nature of embryonic stem cells (ESC) is associated with a dynamic open chromatin state and an irregular nuclear shape. It has been postulated that the absence of Lamin A/C contributes to these features. However, we show that mouse ESCs express low, yet readily detectable, amounts of Lamin A/C at both the RNA and protein levels. Full-length transcripts of both isoforms were readily detected by q-PCR and deep RNA sequencing. Additionally, protein expression was validated in multiple primary and established ESC lines by immunoblotting using several independent antibodies. Immunofluorescence labeling showed localization of Lamin A/C at the nuclear periphery of all Oct4/Nanog double-positive ESC lines examined, as well as in the inner cell mass of blastocysts. Our results demonstrate ESCs do express low levels of Lamin A/C, thus models linking pluripotency and nuclear dynamics with the absence of Lamin A/C need to be revisited.
authors: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY USA.
authors: Melanie A
authors: MA
authors: Eckersley-Maslin
authors: Jan H
authors: JH
authors: Bergmann
authors: Zsolt
authors: Z
authors: Lazar
authors: David L
authors: DL
authors: Spector
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006570:Heterochromatin / Q000378:metabolism; D018398:Homeodomain Proteins / Q000378:metabolism; D034904:Lamin Type A / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000378:metabolism
chemical_list: D006570:Heterochromatin; D018398:Homeodomain Proteins; D034904:Lamin Type A; D000071317:Nanog Homeobox Protein; C475540:Nanog protein, mouse; D050814:Octamer Transcription Factor-3; C440308:lamin C
keywords: Lamin A/C; embryonic stem cell; lamina; nucleus