title: Accuracy profile validation of a new analytical method for butane measurement using headspace-gas chromatography-mass spectrometry.
abstract: The aim of our study was to provide an innovative HS-GC/MS method applicable to the routine determination of butane concentration in forensic toxicology laboratories. The main drawback of the GC/MS methods discussed in literature concerning butane measurement was the absence of a specific butane internal standard necessary to perform quantification. Because no stable isotope of butane is commercially available, it is essential to develop a new approach by an in situ generation of standards. To avoid the manipulation of a stable isotope-labelled gas, we have chosen to generate in situ an internal labelled standard gas (C(4)H(9)D) following the basis of the stoichiometric formation of butane by the reaction of deuterated water (D(2)O) with Grignard reagent butylmagnesium chloride (C(4)H(9)MgCl). This method allows a precise measurement of butane concentration and therefore, a full validation by accuracy profile was presented.
authors: Toxicology and Forensic Chemistry Unit, University Center of Legal Medicine Lausanne - Geneva, CH-1011 Lausanne, Switzerland. vincent.varlet@chuv.ch
authors: V
authors: V
authors: Varlet
authors: F
authors: F
authors: Smith
authors: M
authors: M
authors: Augsburger
mesh_terms: D002073:Butanes / Q000032:analysis*; D002138:Calibration; D003903:Deuterium; D044707:Forensic Sciences; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results
chemical_list: D002073:Butanes; C046888:butane; D003903:Deuterium