title: Increasing l-isoleucine production in Corynebacterium glutamicum by overexpressing global regulator Lrp and two-component export system BrnFE.
abstract: OBJECTIVE
  To increase the l-isoleucine production in Corynebacterium glutamicum by overexpressing the global regulator Lrp and the two-component export system BrnFE.
  
  
  RESULTS
  The brnFE operon and the lrp gene were cloned into the shuttle vector pDXW-8 individually or in combination. The constructed plasmids were transformed into an l-isoleucine-producing strain C. glutamicum JHI3-156, and the l-isoleucine production in these different strains was analysed and compared. More l-isoleucine was produced when only Lrp was expressed than when only BrnFE was expressed. Significant increase in l-isoleucine production was observed when Lrp and BrnFE were expressed in combination. Compared to the control strain, l-isoleucine production in JHI3-156/pDXW-8-lrp-brnFE increased 63% in flask cultivation, and the specific yield of l-isoleucine increased 72% in fed-batch fermentation.
  
  
  CONCLUSIONS
  Both Lrp and BrnFE are important to enhance the l-isoleucine production in C. glutamicum.
  
  
  CONCLUSIONS
  The results provide useful information to enhance l-isoleucine or other branched-chain amino acid production in C. glutamicum.
authors: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China.
authors: L
authors: L
authors: Yin
authors: F
authors: F
authors: Shi
authors: X
authors: X
authors: Hu
authors: C
authors: C
authors: Chen
authors: X
authors: X
authors: Wang
mesh_terms: D061249:Batch Cell Culture Techniques; D048230:Corynebacterium glutamicum / Q000235:genetics* / Q000378:metabolism*; D005285:Fermentation; D005818:Genetic Engineering; D005822:Genetic Vectors; D007532:Isoleucine / Q000096:biosynthesis*; D051756:Leucine-Responsive Regulatory Protein / Q000235:genetics; D009876:Operon / Q000235:genetics; D010957:Plasmids
chemical_list: D007532:Isoleucine; D051756:Leucine-Responsive Regulatory Protein