title: Vital staining of nerve structures with fluorescent dyes and optical determination of acetylcholine in the somatic muscle of the earthworm Lumbricus terrestris.
abstract: Experiments with fluorescent dyes showed that high concentrations of K(+) ions in the medium depolarize the membrane and enhance exo-endocytosis in nerve structures, which is accompanied by an increase in acetylcholine concentration in the somatic muscle of earthworm. In the presence of BAPTA and without Ca(2+) exo-endocytosis is sharply decelerated, the level of acetylcholine in the muscle decreases, but remains relatively high.
authors: Kazan State Medical University, Ministry of Health and Social Development of the Russian Federation, Russia. euroworm@mail.ru
authors: M E
authors: ME
authors: Volkov
mesh_terms: D000109:Acetylcholine / Q000032:analysis*; D000818:Animals; D002118:Calcium / Q000032:analysis; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D004705:Endocytosis; D005089:Exocytosis; D005456:Fluorescent Dyes; D009132:Muscles / Q000737:chemistry / Q000294:innervation; D009417:Nerve Tissue / Q000737:chemistry; D009835:Oligochaeta / Q000737:chemistry*; D011188:Potassium / Q000032:analysis; D017729:Presynaptic Terminals / Q000737:chemistry* / Q000648:ultrastructure; D013194:Staining and Labeling
chemical_list: D005456:Fluorescent Dyes; D004533:Egtazic Acid; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; D000109:Acetylcholine; D011188:Potassium; D002118:Calcium