title: Mir143 expression inversely correlates with nuclear ERK5 immunoreactivity in clinical prostate cancer.
abstract: BACKGROUND
  Aberrant mitogen/extracellular signal-regulated kinase 5 (MEK5)-extracellular signal-regulated protein kinase 5 (ERK5)-mediated signalling has been implicated in a number of tumour types including prostate cancer (CaP). The mechanism for ERK5 activation in CaP remains to be fully elucidated. Studies have recently implicated the role of microRNA (miRNA) mir143 expression in the regulation of ERK5 expression.
  
  
  METHODS
  We utilised a tissue microarray (TMA) of 530 CaP cores from 168 individual patients and stained for both mir143 and ERK5. These TMAs were scored by a combination of observer and automated methods.
  
  
  RESULTS
  We observed a strong inverse relation between ERK5 and mir143, which manifested itself most strongly in the subgroup of 417 cores with non-zero mir143 and ERK5 immunoreactivity, or with only one of mir143 or ERK5 being zero (cc=0.2558 and P<0.0001). Mir143 neither correlate with Gleason scores or prostate-specific antigen levels, nor was it a predictor of disease-specific survival on univariate analysis.
  
  
  CONCLUSIONS
  Although the mechanism for ERK5 activation in CaP remains to be fully elucidated, we have further validated the potential role of mir143 in regulating ERK5 levels in the clinical context. In addition, we demonstrate that the automated counting method for nuclear ERK5 is a clinically useful alterative to observer counting method in patient stratification in the context of ERK5 targeting therapy.
authors: Beatson Institute for Cancer Research, Bearsden, Glasgow G61 1BD, UK.
authors: I
authors: I
authors: Ahmad
authors: L B
authors: LB
authors: Singh
authors: Z H
authors: ZH
authors: Yang
authors: G
authors: G
authors: Kalna
authors: J
authors: J
authors: Fleming
authors: G
authors: G
authors: Fisher
authors: C
authors: C
authors: Cooper
authors: J
authors: J
authors: Cuzick
authors: D M
authors: DM
authors: Berney
authors: H
authors: H
authors: Møller
authors: P
authors: P
authors: Scardino
authors: H Y
authors: HY
authors: Leung
mesh_terms: D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D048054:Mitogen-Activated Protein Kinase 7 / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism
chemical_list: C542359:MIRN143 microRNA, human; D035683:MicroRNAs; D048054:Mitogen-Activated Protein Kinase 7