title: Changes in the Archaea microbial community when the biogas fermenters are fed with protein-rich substrates.
abstract: Terminal restriction fragment length polymorphism (T-RFLP) was applied to study the changes in the composition of the methanogens of biogas-producing microbial communities on adaptation to protein-rich monosubstrates such as casein and blood. Specially developed laboratory scale (5-L) continuously stirred tank reactors have been developed and used in these experiments. Sequencing of the appropriate T-RF fragments selected from a methanogen-specific (mcrA gene-based) library revealed that the methanogens responded to the unconventional substrates by changing the community structure. T-RFLP of the 16S rDNA gene confirmed the findings.
authors: Department of Biotechnology, University of Szeged, H-6726 Szeged, Közép fasor 52, Hungary. acsn@brc.hu
authors: Norbert
authors: N
authors: Ács
authors: Etelka
authors: E
authors: Kovács
authors: Roland
authors: R
authors: Wirth
authors: Zoltán
authors: Z
authors: Bagi
authors: Orsolya
authors: O
authors: Strang
authors: Zsófia
authors: Z
authors: Herbel
authors: Gábor
authors: G
authors: Rákhely
authors: Kornél L
authors: KL
authors: Kovács
mesh_terms: D001105:Archaea / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D061249:Batch Cell Culture Techniques / Q000379:methods*; D056804:Biofuels / Q000382:microbiology*; D019149:Bioreactors / Q000382:microbiology*; D008697:Methane / Q000302:isolation & purification / Q000378:metabolism*; D059013:Microbial Consortia / Q000502:physiology*; D011506:Proteins / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D056804:Biofuels; D011506:Proteins; D008697:Methane