title: Isolation of clonal cultures of endosymbiotic green algae from their ciliate hosts.
abstract: Using Paramecium bursaria as a model organism improved protocols have been developed to isolate clonal endosymbiotic algae. This involved micromanipulation of individual protists, rupturing to release endosymbionts followed by enrichment on complex media and a series of plating steps, under low light (PAR ~10μmol photons m(-2)s(-1)).
authors: Culture Collection of Algae and Protozoa, Scottish Association for Marine Science, Scottish Marine Institute, Oban, Argyll, PA37 1QA, UK. uad@sams.ac.uk
authors: Undine E M
authors: UE
authors: Achilles-Day
authors: John G
authors: JG
authors: Day
mesh_terms: D000460:Chlorophyta / Q000502:physiology*; D008828:Microbiological Techniques / Q000379:methods*; D010247:Paramecium / Q000382:microbiology*; D013559:Symbiosis