title: Expression, purification, and characterization of a diabody against the most important angiogenesis cell receptor: Vascular endothelial growth factor receptor 2.
abstract: Antibodies and their derivative fragments have long been used as tools in a variety of applications, in fundamental research work, biotechnology, diagnosis, and therapy. Camels produce single heavy-chain antibodies (VHH) in addition to usual antibodies. These minimal-sized binders are very robust and bind the antigen with high affinity in a monomeric state. Vascular endothelial growth factor recepror-2 (VEGFR2) is an important tumor-associated receptor that blockade of its signaling can lead to the inhibition of neovascularization and tumor metastasis. Here, we describe the construction, expression, and purification VEGFR2-specific Diabody. Two variable fragments of a same camel anti-VEGFR2 antibody were linked together by the upper hinge segment of antibody to make a diabody. We showed the ability of diabody to recognition of VEGFR2 on the cell surface by FACS. Diabodies can be produced in the low-cost prokaryotic expression system, so they are suitable molecules for diagnostic and therapeutic issues.
authors: Department of Molecular Medicine, Pasteur Institute of Iran, Tehran, Iran.
authors: Mahdi
authors: M
authors: Behdani
authors: Sirous
authors: S
authors: Zeinali
authors: Morteza
authors: M
authors: Karimipour
authors: Hossein
authors: H
authors: Khanahmad
authors: Nader
authors: N
authors: Asadzadeh
authors: Kayhan
authors: K
authors: Azadmanesh
authors: Negar
authors: N
authors: Seyed
authors: Seyed Farzad
authors: SF
authors: Baniahmad
authors: Mahdi Habibi
authors: MH
authors: Anbouhi
keywords: Diabody; Nanobody; vascular endothelial growth factor recepror-2