title: Novel fluorescent risedronates: synthesis, photodynamic inactivation and imaging of Bacillus subtilis.
abstract: Novel fluorescently-labeled conjugates of risedronate were synthesized using an epoxide linker, enabling conjugation of risedronate via its pyridyl nitrogen with the aromatic succinimidyl esters. The compounds were characterized by using (1)H NMR, (13)C NMR, (31)P NMR, UV-vis and fluorescence emission spectroscopies. Biological activity assays showed that the conjugates 14 and 15 exhibited photodynamic inactivation of Bacillus subtilis (ATCC 6633) with 91% and 47% bacterial lethality at 10 μM upon visible light irradiation, respectively. Both 14 and 15 could be also used for fluorescence imaging of Bacillus subtilis.
authors: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, PR China.
authors: Li-Sheng
authors: LS
authors: Zhou
authors: Ke-Wu
authors: KW
authors: Yang
authors: Lei
authors: L
authors: Feng
authors: Jian-Min
authors: JM
authors: Xiao
authors: Cheng-Cheng
authors: CC
authors: Liu
authors: Yi-Lin
authors: YL
authors: Zhang
authors: Michael W
authors: MW
authors: Crowder
mesh_terms: D001412:Bacillus subtilis / Q000737:chemistry* / Q000187:drug effects; D012968:Etidronic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D005452:Fluoresceins / Q000138:chemical synthesis / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D018613:Microscopy, Confocal; D063065:Organophosphonates / Q000138:chemical synthesis / Q000737:chemistry; D017319:Photosensitizing Agents / Q000737:chemistry / Q000494:pharmacology; D000068296:Risedronic Acid; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D063065:Organophosphonates; D017319:Photosensitizing Agents; D000068296:Risedronic Acid; D012968:Etidronic Acid