title: Chromatofocusing purification of CD1b-antigen complexes and their analysis by isoelectric focusing.
abstract: The presentation of lipid antigens to T cells is mediated by the CD1 proteins. Purified functional CD1/lipid complexes are valuable tools to investigate such immune processes. Here, we describe how these complexes can be prepared in vitro, how they can be purified by chromatofocusing and how to control their antigen-loading status by isoelectric focusing.
authors: Institut de Pharmacologie et de Biologie Structurale (IPBS), UMR 5089 CNRS/Université Toulouse III-Paul Sabatier, Toulouse, France. luis-fernando.garcia-alles@ipbs.fr.
authors: Luis Fernando
authors: LF
authors: Garcia-Alles
authors: Biologie des cellules dendritiques humaines, U725 Inserm/Université de Strasbourg/Etablissement Français du Sang-Alsace, Strasbourg, France. henri.delasalle@efs-alsace.fr.
authors: Henri
authors: H
authors: de la Salle
mesh_terms: D018949:Antigens, CD1 / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D017434:Protein Structure, Tertiary
chemical_list: D018949:Antigens, CD1; C419394:CD1b antigen