title: Assessment of tracer 99mTc(V)-DMSA uptake as a measure of tumor cell proliferation in vitro.
abstract: OBJECTIVE
  To examine whether (99m)Tc(V)-DMSA could be used as a non-invasive measure of cancer cell proliferation.
  
  
  METHODS
  Human breast cancer MCF-7, MDA-MB-231 and pII, and prostate cancer PC-3 cell lines were grown to 30, 50 and 100% confluency and pulsed with (99m)Tc(V)-DMSA in media for 60 min at 37°C. DNA synthesis was analysed by quantification of the S phase using flow cytometry, [methyl-(3)H]thymidine incorporation and expression of proliferation markers PCNA and Ki-67 using realtime PCR. One way ANOVA was used to compare groups.
  
  
  RESULTS
  In all cell lines rates of (99m)Tc(V)-DMSA uptake were inversely related to cell density. This was paralleled by similar trends in S phase proportions, [methyl-(3)H]thymidine incorporation and expression of PCNA and Ki-67.
  
  
  CONCLUSIONS
  Rates of (99m)Tc(V)-DMSA uptake into different types of tumour cells correlate well with cell density that is useful as a non-invasive measure of tumour cellular proliferation in vivo.
authors: Nuclear Medicine Department, Faculty of Medicine, Health Sciences Center, Kuwait University, Hawally, Jabriya, Kuwait. fatimas@hsc.edu.kw
authors: Fatma J
authors: FJ
authors: Al-Saeedi
authors: Princy M
authors: PM
authors: Mathew
authors: Yunus A
authors: YA
authors: Luqmani
mesh_terms: D001549:Benzamides / Q000494:pharmacology; D015415:Biomarkers; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D019788:Fluorodeoxyglucose F18 / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D000068877:Imatinib Mesylate; D061986:MCF-7 Cells; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism; D010879:Piperazines / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D019275:Radiopharmaceuticals / Q000378:metabolism*; D016196:S Phase / Q000187:drug effects; D019783:Technetium Tc 99m Dimercaptosuccinic Acid / Q000378:metabolism*
chemical_list: D001549:Benzamides; D015415:Biomarkers; D010879:Piperazines; D011743:Pyrimidines; D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18; D019783:Technetium Tc 99m Dimercaptosuccinic Acid; D000068877:Imatinib Mesylate