title: Discordant genotypic resistance and HIV-1 genetic diversity from paired plasma and cerebrospinal fluid samples in Chinese settings.
abstract: In the highly active antiretroviral therapy era, the incidence of human immunodeficiency virus (HIV)-associated neurocognitive disorder remains high with the improved survival. The prevalence of resistance differs across geographical areas and HIV subtypes. Currently, little information on the resistance patterns in the central nervous system (CNS) is available in Chinese settings. In this study, we sequenced and analyzed the pol gene from paired cerebrospinal fluid and plasma samples of 34 Chinese HIV-infected patients. We found that eight subjects harbored mutations that confer drug resistance, and of these, six subjects exhibited discordant resistance patterns between the CNS and the blood. The levels of viral diversity in the CNS were significantly higher than those in the blood (p < 0.0001). Our results contribute to improving our understanding of HIV neuropathogenesis and help to optimize neuro-acquired immunodeficiency syndrome treatment.
authors: Center for Infectious Diseases, Beijing You An Hospital, Capital Medical University, Beijing, China.
authors: Lifeng
authors: L
authors: Liu
authors: Yulin
authors: Y
authors: Zhang
authors: Feili
authors: F
authors: Wei
authors: Qingxia
authors: Q
authors: Zhao
authors: Xicheng
authors: X
authors: Wang
authors: Lin
authors: L
authors: Yuan
authors: Ning
authors: N
authors: Li
authors: Dexi
authors: D
authors: Chen
mesh_terms: D015526:AIDS Dementia Complex / Q000097:blood / Q000134:cerebrospinal fluid / Q000188:drug therapy*; D019380:Anti-HIV Agents / Q000627:therapeutic use*; D023241:Antiretroviral Therapy, Highly Active; D001483:Base Sequence; D002490:Central Nervous System / Q000821:virology; D002681:China; D024882:Drug Resistance, Viral / Q000187:drug effects / Q000235:genetics*; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D018740:Genetic Heterogeneity; D015497:HIV-1 / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D019562:Viral Load; D054299:env Gene Products, Human Immunodeficiency Virus / Q000097:blood / Q000134:cerebrospinal fluid / Q000235:genetics*; D054302:pol Gene Products, Human Immunodeficiency Virus / Q000097:blood / Q000134:cerebrospinal fluid / Q000235:genetics*
chemical_list: D019380:Anti-HIV Agents; D054299:env Gene Products, Human Immunodeficiency Virus; D054302:pol Gene Products, Human Immunodeficiency Virus