title: Effects of HSP27 chaperone on THP-1 tumor cell apoptosis.
abstract: The role of Hsp27 (heat shock protein 27) chaperone in regulation of THP-1 tumor cell apoptosis was studied. Realization of tumor cell apoptosis under conditions of in vitro culturing with Hsp27 specific inhibitor (KRIBB3) was evaluated by fluorescent microscopy with FITC-labeled annexin V and propidium iodide. Measurements of Bcl-2 family proteins (Bcl-2, Bax, Bad) in tumor cells incubated with Hsp27 inhibitor were carried out by Western blotting. Chaperone Hsp27 acted as apoptosis inhibitor in THP-1 tumor cells modulating the proportion of antiapoptotic (Bcl-2) and proapoptotic (Bax and Bad) proteins.
authors: Department of Fundamental Clinical Medicine, Research and Educational Center of Molecular Medicine, Siberian State Medical University, the Ministry of Health and Social Development of the Russian Federation, Tomsk, Russia. zlobinae@mail.ru
authors: E V
authors: EV
authors: Kaigorodova
authors: N V
authors: NV
authors: Ryazantseva
authors: V V
authors: VV
authors: Novitskii
authors: A N
authors: AN
authors: Maroshkina
authors: M V
authors: MV
authors: Belkina
mesh_terms: D000293:Adolescent; D000328:Adult; D000840:Anisoles / Q000494:pharmacology*; D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins; D045744:Cell Line, Tumor; D005260:Female; D055551:HSP27 Heat-Shock Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006801:Humans; D007555:Isoxazoles / Q000494:pharmacology*; D007938:Leukemia / Q000473:pathology*; D007963:Leukocytes, Mononuclear; D008297:Male; D008875:Middle Aged; D018832:Molecular Chaperones / Q000378:metabolism; D055017:Monocyte-Macrophage Precursor Cells / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D055815:Young Adult
chemical_list: C507623:5-(5-ethyl-2-hydroxy-4-methoxyphenyl)-4-(4-methoxyphenyl)isoxazole; D000840:Anisoles; D017304:Annexin A5; D051017:Apoptosis Regulatory Proteins; D055551:HSP27 Heat-Shock Proteins; D007555:Isoxazoles; D018832:Molecular Chaperones; D019253:Proto-Oncogene Proteins c-bcl-2