title: Development of culture medium using extruded bean as a nitrogen source for yeast growth.
abstract: In this study extruded bean was used as a nitrogen source substitute in culture medium formulation. A 3-factor simplex-lattice mixture design was used to establish better growth conditions. Completely substituted medium resulted in 43% of increase in the growth of Saccharomyces cerevisiae. Mixtures containing 1% extruded bean and 1% yeast extract, or 1% extruded bean and 1% peptone presented growths of 76-79% higher than the commercial YPD medium for S. cerevisiae. Pichia pastoris (GS115) growth was enhanced by 20% using a completely substituted medium. The protein expression patterns in P. pastoris (GS115) remained unchanged when growth was conducted in a medium containing extruded bean as unique nitrogen source. The total amount of recombinant protein expressed in extruded bean medium was 88.5% higher than in control expression medium. These results evidenced that extruded bean can be successfully used as a substitute of peptone and yeast extract in culture media for S. cerevisiae's and P. pastoris' (GS115) growth.
authors: Laboratório de Química de Proteínas, Departamento de Bioquímica e Biologia Molecular, Instituto de Ciências Biológicas, ICB II, Universidade Federal de Goiás, Brazil. karla-batista@hotmail.com
authors: Karla A
authors: KA
authors: Batista
authors: Luiz Artur M
authors: LA
authors: Bataus
authors: Ivan T N
authors: IT
authors: Campos
authors: Kátia F
authors: KF
authors: Fernandes
mesh_terms: D003470:Culture Media / Q000737:chemistry*; D007887:Fabaceae / Q000737:chemistry*; D009172:Mycology / Q000379:methods; D009584:Nitrogen / Q000378:metabolism*; D010843:Pichia / Q000254:growth & development* / Q000378:metabolism; D010936:Plant Extracts / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000254:growth & development* / Q000378:metabolism
chemical_list: D003470:Culture Media; D010936:Plant Extracts; D011994:Recombinant Proteins; D009584:Nitrogen