title: The edible mushroom Laetiporus sulphureus as potential source of natural antioxidants.
abstract: Hot water extract (LN), partially purified polysaccharides (LP) and hot alkali extracted polysaccharides (LNa) obtained from fruiting bodies of the wild basidiomycete Laetiporus sulphureus were examined for their antioxidant activities. LNa was the most active antioxidant, as shown by the median effective concentrations (EC50 values) of 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity (0.5 ± 0.2 mg/ml), reducing power (4.0 ± 0.3 mg/ml) and ferrous ion-chelating ability (1.5 ± 0.1 mg/ml). LNa contained the highest level of α-glucan (17.3 ± 1.2 g/100 g dw), whereas LP contained mostly β-glucans (66.8 ± 1.3 g/100 g dw). The prevalent monosaccharide in all extracts was glucose. The EC50 values of all three antioxidant activity assays were well-correlated with the α-glucan content. Strong and significant correlation was found between total phenolic compounds and DPPH scavenging ability and also reducing power. The three investigated extracts (at concentrations of 0.1-10 mg/ml) were not toxic to HTR-8/SVneo trophoblast cell line.
authors: Department for Industrial Microbiology, Faculty of Agriculture, Institute for Food Technology and Biochemistry, University of Belgrade, Nemanjina 6, Belgrade 11080, Serbia.
authors: Anita
authors: A
authors: Klaus
authors: Maja
authors: M
authors: Kozarski
authors: Miomir
authors: M
authors: Niksic
authors: Dragica
authors: D
authors: Jakovljevic
authors: Nina
authors: N
authors: Todorovic
authors: Ivana
authors: I
authors: Stefanoska
authors: Leo J L D
authors: LJ
authors: Van Griensven
mesh_terms: D000363:Agaricales; D000975:Antioxidants / Q000494:pharmacology*; D001487:Basidiomycota / Q000737:chemistry*; D001688:Biological Products / Q000737:chemistry / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology; D005296:Ferrous Compounds / Q000378:metabolism; D048690:Fruiting Bodies, Fungal; D005936:Glucans / Q000494:pharmacology*; D005947:Glucose / Q000032:analysis; D010636:Phenols / Q000494:pharmacology*; D010851:Picrates / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001688:Biological Products; D001713:Biphenyl Compounds; D002614:Chelating Agents; D005296:Ferrous Compounds; D005936:Glucans; D010636:Phenols; D010851:Picrates; D011134:Polysaccharides; C004931:1,1-diphenyl-2-picrylhydrazyl; D005947:Glucose