title: Multilayer microfluidic poly(ethylene glycol) diacrylate hydrogels.
abstract: Development of robust, in vivo like tissues in vitro holds the potential to create regenerative medicine-based therapeutics, provide more physiologically significant preclinical models and supply a pharmacological and toxicological screening platform that reflects in vivo systems in both complexity and function. This protocol describes a simple, robust, multilayer replica molding technique in which poly(dimethylsiloxane) (PDMS) and poly(ethylene glycol) diacrylate (PEGDA) are serially replica molded to develop microfluidic PEGDA hydrogel networks embedded within independently fabricated PDMS housings, using a combination of soft and photo-lithography. This work has direct applications toward the development of robust, complex, cell-laden hydrogels for in vitro diagnostics and regenerative medicine applications.
authors: Department of Bioengineering, MS-142, BRC, Rice University, Houston, TX, USA.
authors: Michael P
authors: MP
authors: Cuchiara
authors: Jennifer L
authors: JL
authors: West
mesh_terms: D020100:Hydrogels / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011327:Printing
chemical_list: D020100:Hydrogels; C437167:poly(ethylene glycol)diacrylate; D011092:Polyethylene Glycols