title: Metabolic profile of keratoconic cornea.
abstract: OBJECTIVE
  To investigate the difference in metabolic profile of keratoconic and normal corneas using two different analysis methods.
  
  
  METHODS
  Keratoconic corneas were obtained from patients (aged 19-27) during transplantation surgery. Control samples were obtained from human donors (aged 61-75) 1-8 h post-mortem. The metabolic profile of tissues was investigated with high-resolution magic angle spinning (1)H nuclear magnetic resonance (NMR) spectroscopy and high performance liquid chromatography (HPLC).
  
  
  RESULTS
  Nine amino acids and 20 metabolites were assigned with HPLC and NMR spectroscopy, respectively. No significant biochemical difference was revealed between keratoconic and control samples, which represent distant age groups.
  
  
  CONCLUSIONS
  It suggests that development of keratoconus might be related to the accelerated ageing of the cornea. This issue warrants further studies.
authors: Department of Neuroscience, Faculty of Medicine, Norwegian University of Science and Technology, Trondheim, Norway. kryczka.tomasz@gmail.com
authors: Tomasz
authors: T
authors: Kryczka
authors: Niels
authors: N
authors: Ehlers
authors: Kim
authors: K
authors: Nielsen
authors: Edward
authors: E
authors: Wylegala
authors: Dariusz
authors: D
authors: Dobrowolski
authors: Anna
authors: A
authors: Midelfart
mesh_terms: D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003315:Cornea / Q000378:metabolism* / Q000473:pathology; D016039:Corneal Transplantation; D005260:Female; D006801:Humans; D007640:Keratoconus / Q000378:metabolism* / Q000473:pathology / Q000601:surgery*; D008297:Male; D055442:Metabolome; D008875:Middle Aged; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011522:Protons; D014019:Tissue Donors; D055815:Young Adult
chemical_list: D011522:Protons