title: Docosahexaenoic acid (DHA) induces apoptosis in human hepatocellular carcinoma cells.
abstract: The docosahexaenoic (DHA), a ω-3 fatty acid, could play a beneficial inhibition of the incidence and progress of a series of human diseases including cancer. It has been report that DHA is involved in cell apoptosis. Recent studies show that the signal transduction pathway links with bcl-2, bax, caspase-3 and MMP-9 molecules. Therefore, we tested the relationship between DHA and cell apoptosis in human hepatocellular carcinoma cells (Bel-7402 cells). We show here that DHA induces Bel-7402 cells apoptosis after pre-treating cells with DHA. DHA down-regulates the protein expression of Bcl-2 and Bim mRNA level, and up-regulates caspase-3 activity and Bax expression level. We also found that DHA inhibits Bel-7402 cells migration. Basic on our studies, DHA may play a role in tumor invasion and survival.
authors: Department of General Surgery, Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, Anhui Provincial Hospital Affiliated with Anhui Medical University No. 17 Lujiang Road, Hefei 230001, Anhui Province, China.
authors: Si-Nan
authors: SN
authors: Sun
authors: Wei-Dong
authors: WD
authors: Jia
authors: Hao
authors: H
authors: Chen
authors: Jin-Liang
authors: JL
authors: Ma
authors: Yong-Sheng
authors: YS
authors: Ge
authors: Ji-Hai
authors: JH
authors: Yu
authors: Jian-Sheng
authors: JS
authors: Li
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D053148:Caspase 3 / Q000378:metabolism; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494826:BAX protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D004281:Docosahexaenoic Acids; C505407:CASP3 protein, human; D053148:Caspase 3; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: Bcl-2; Docosahexaenoic (DHA); MMP-9; caspase-3