title: Early detection of renal injury using urinary vanin-1 in rats with experimental colitis.
abstract: Renal complications are often detected in patients with inflammatory bowel disease (IBD). Because conventional markers such as serum creatinine and β2-microglobulin are not sensitive and/or specific, a new renal biomarker is needed. We have recently identified urinary vanin-1 as an early biomarker for the detection of nephrotoxicant-induced renal injury. In this study, we compared the usefulness of urinary vanin-1 with other newly developed biomarkers [urinary monocyte chemoattractant protein-1 (MCP-1), kidney injury molecule-1 (Kim-1) and N-acetyl-beta-D-glucosaminidase (NAG)] for the detection of renal complications in rats with experimental colitis. On day 2 after intracolonic injection of 2,4,6-trinitrobenzene sulfonic acid (TNBS), male Wistar rats developed not only colitis, but histologically evident renal injury. Urinary vanin-1 started to elevate on day 1, whereas serum creatinine and urinary excretions of glucose, total protein, albumin, Kim-1, MCP-1 and NAG significantly increased only on day 2. The mRNA expressions of vanin-1 and Kim-1 significantly increased in the kidney, but not in the colon. In addition, vanin-1 did not appear in the blood. On the other hand, colonic mRNA expression and the serum concentration of MCP-1 were significantly higher in the TNBS-treated rats than in the control animals. These results suggest that, in contrast to MCP-1, urinary vanin-1 and Kim-1 mainly originated from the kidney rather than the colon in this model. Compared with Kim-1 and MCP-1, vanin-1 might be an earlier biomarker for the detection of renal injury in rats with experimental colitis.
authors: Division of Clinical Pharmacology, Department of Pharmacology, School of Medicine, Jichi Medical University, Tochigi, 329-0498, Japan.
authors: Keiko
authors: K
authors: Hosohata
authors: Hitoshi
authors: H
authors: Ando
authors: Akio
authors: A
authors: Fujimura
mesh_terms: D000118:Acetylglucosaminidase / Q000652:urine; D000581:Amidohydrolases / Q000652:urine*; D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000652:urine; D015815:Cell Adhesion Molecules / Q000652:urine; D018932:Chemokine CCL2 / Q000652:urine; D003092:Colitis / Q000139:chemically induced / Q000473:pathology / Q000652:urine*; D003106:Colon / Q000187:drug effects / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D042241:Early Diagnosis; D058851:GPI-Linked Proteins / Q000652:urine; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D007674:Kidney Diseases / Q000175:diagnosis*; D007677:Kidney Function Tests; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014302:Trinitrobenzenesulfonic Acid
chemical_list: D000118:Acetylglucosaminidase; D000581:Amidohydrolases; D015415:Biomarkers; D015815:Cell Adhesion Molecules; D018932:Chemokine CCL2; D003404:Creatinine; D058851:GPI-Linked Proteins; D012333:RNA, Messenger; D014302:Trinitrobenzenesulfonic Acid; C491766:Ccl2 protein, rat; C110936:Havcr1 protein, rat; C022314:pantetheinase
keywords: TNBS; experimental model; inflammatory bowel disease; renal complications; urinary biomarker