title: Correlation between Rho-kinase pathway gene expressions and development and progression of glioblastoma multiforme.
abstract: Glioblastoma multiforme (GBM) is the most common and the most aggressive primary malignant tumor of the brain. Prognostic factors in GBM can be sorted as age, tumor localization, tumor diameter, symptom period and type, the extent of surgery, postoperative tumor volume, and adjuvant radiotherapy and/or chemotherapy status. Besides the interactions between actin microfilaments, microtubules, and intermediate filaments, environmental factors and intracellular signals which regulate them affect the cell invasion. Rho proteins and therefore Rho-kinase activation play important role at these changes. The aim of this study is to evaluate the relationship between the Rho-kinase pathway gene expressions and prognosis in GBM. Ninety-eight patients diagnosed as GBM between 2001 and 2010 were enrolled into the study. RNA was obtained from the paraffinized tumor tissue of the patients with formalin-fixed, paraffin-embedded RNA isolation kit and the mRNA expressions of 26 genes were investigated. There was a statistically significant negative correlation between the ages at the diagnosis and survival. There was a significant relationship between the overexpression of Rho-kinase pathway-related genes LIMK1, CFL1, CFL2, and BCL2 and low expression of MAPK1 gene and the survival of the patients. These results demonstrate for the first time that there is a marked contribution of Rho-kinase pathway-related genes to the progression and survival of the GBM. The expression of these genes may be related to response of multimodal therapy or these parameters could be used to determine possible unresponsive patients before treatment.
authors: Department of Neurosurgery, School of Medicine, Gaziantep University, 27310, Gaziantep, Turkey.
authors: Ibrahim
authors: I
authors: Erkutlu
authors: Ahmet
authors: A
authors: Cigiloglu
authors: Mehmet Emin
authors: ME
authors: Kalender
authors: Mehmet
authors: M
authors: Alptekin
authors: A Tuncay
authors: AT
authors: Demiryurek
authors: Ali
authors: A
authors: Suner
authors: Esma
authors: E
authors: Ozkaya
authors: Mustafa
authors: M
authors: Ulasli
authors: Celalettin
authors: C
authors: Camci
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D016022:Case-Control Studies; D051338:Cofilin 1 / Q000235:genetics / Q000378:metabolism; D051340:Cofilin 2 / Q000235:genetics / Q000378:metabolism; D005260:Female; D005909:Glioblastoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D054461:Lim Kinases / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics / Q000378:metabolism; D011379:Prognosis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D054460:rho-Associated Kinases / Q000235:genetics* / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; C495810:CFL1 protein, human; C495815:CFL2 protein, human; D051338:Cofilin 1; D051340:Cofilin 2; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; C515892:LIMK1 protein, human; D054461:Lim Kinases; D054460:rho-Associated Kinases; C535150:MAPK1 protein, human; D019950:Mitogen-Activated Protein Kinase 1