title: [Transfer and identification of multidrug resistance gene in mesenchymal stem cells derived from human placenta].
abstract: OBJECTIVE
  To explore the feasibility and safety of mdr1 gene transferred into placenta derived mesenchymal stem cells (P-MSCs) by reconstructed retroviral vector.
  
  
  METHODS
  Human P-MSCs were isolated and expanded by Percoll density gradient and then transduced repeatedly by reconstructed retroviral vector containing mdr1 gene. The transfection and expression of mdr1 gene was detected by reverse transcription-polymerase chain reaction (RT-PCR) and fluorescence-activated cell sorting (FACS). Meanwhile, the biological features of mdr1-MSCs were identified and analyzed.
  
  
  RESULTS
  The expression of mdr1mRNA was found in transfected cells. The expression of P-glycoprotein (P-gp) encoded by mdr1 gene was (27.6 ± 5.1)% in the transfected P-MSCs cells versus (0.4 ± 0.1)% in the non-transfected P-MSCs cells (t = 14.291, P < 0.01). The percent of P-MSCs at quiescent phase (G0/G1 phase) was around 95.40% and it was in accord with the characterization of stem cells. The mdr1-MSCs exhibited typical ultrastructures of low-differentiated stem cells. Moreover, they still retained the potency of adipogenic and osteogenic differentiation in the presence of appropriate conditioned media.
  
  
  CONCLUSIONS
  A stable expression of P-gp may be obtained by reconstructed retroviral-mediated transfection in vitro. And transfected MSCs retain the characteristics of stem cells.
authors: Department of Obstetrics & Gynecology, Second Hospital, Jilin University, Changchun 130041, China.
authors: Li-ying
authors: LY
authors: Han
authors: Ya-ping
authors: YP
authors: Li
authors: Ming-zhu
authors: MZ
authors: Ye
authors: Bo-wei
authors: BW
authors: Wang
authors: Qiang
authors: Q
authors: Wang
authors: Shu-hua
authors: SH
authors: Zhao
authors: He-lian
authors: HL
authors: Li
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics; D002478:Cells, Cultured; D018432:Drug Resistance, Multiple / Q000235:genetics*; D005260:Female; D019450:Genes, MDR; D005822:Genetic Vectors; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D014162:Transfection
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1