title: Quantitative glycomics strategies.
abstract: The correlations between protein glycosylation and many biological processes and diseases are increasing the demand for quantitative glycomics strategies enabling sensitive monitoring of changes in the abundance and structure of glycans. This is currently attained through multiple strategies employing several analytical techniques such as capillary electrophoresis, liquid chromatography, and mass spectrometry. The detection and quantification of glycans often involve labeling with ionic and/or hydrophobic reagents. This step is needed in order to enhance detection in spectroscopic and mass spectrometric measurements. Recently, labeling with stable isotopic reagents has also been presented as a very viable strategy enabling relative quantitation. The different strategies available for reliable and sensitive quantitative glycomics are herein described and discussed.
authors: Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas 79409, USA. yehia.mechref@ttu.edu
authors: Yehia
authors: Y
authors: Mechref
authors: Yunli
authors: Y
authors: Hu
authors: Janie L
authors: JL
authors: Desantos-Garcia
authors: Ahmed
authors: A
authors: Hussein
authors: Haixu
authors: H
authors: Tang
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002853:Chromatography, Liquid; D019075:Electrophoresis, Capillary / Q000379:methods; D054794:Glycomics; D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D040901:Proteomics; D013050:Spectrometry, Fluorescence / Q000379:methods; D013194:Staining and Labeling
chemical_list: D006023:Glycoproteins; D011134:Polysaccharides