title: Bleomycin delivery into cancer cells in vitro with ultrasound and SonoVue® or BR14® microbubbles.
abstract: BACKGROUND
  Cell exposure to ultrasound (US) in the presence of contrast agent microbubbles (MBs) can result in cell sonoporation that can be exploited for drug or gene delivery. Anticancer drug bleomycin (BLM), used in sonoporation, can effectively eliminate tumor cells in vitro and in vivo. Nevertheless, sonoporation mechanism is not known, thus different US parameters and MB types are used. Recently, we proposed that efficiency of cell sonoporation can be related to the efficiency of MB sonodestruction.
  
  
  OBJECTIVE
  We analyzed human tumor cells viability in response to BLM, US and MB treatment.
  
  
  METHODS
  Human glioblastoma astrocytoma (U-87 MG) or colon cancer (HCT-116) cells were exposed to US in the presence of BLM and either SonoVue® or BR14® MBs. MB sonodestruction was evaluated according to US signal attenuation.
  
  
  RESULTS
  Both HCT-116 and U-87 MG cell viability following US exposure decreased up to 30%. Decrease in cell viability followed similar tendency as MB sonodestruction, which suggests direct relationship between MB sonodestruction and BLM intracellular delivery.
  
  
  CONCLUSIONS
  Sonoporation is a feasible method to deliver BLM in to several types of human cancer cell lines. Efficiency of cell sonoporation correlated well with MB sonodestruction, providing a possibility to optimize US parameters by measuring MB sonodestruction.
authors: Department of Biology, Faculty of Natural Sciences, Vytautas Magnus University, Kaunas, Lithuania.
authors: N
authors: N
authors: Lamanauskas
authors: A
authors: A
authors: Novell
authors: J-M
authors: JM
authors: Escoffre
authors: M
authors: M
authors: Venslauskas
authors: S
authors: S
authors: Satkauskas
authors: A
authors: A
authors: Bouakaz
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry; D001692:Biological Transport; D001761:Bleomycin / Q000008:administration & dosage* / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003287:Contrast Media / Q000008:administration & dosage / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D045325:HCT116 Cells; D006801:Humans; D045423:Microbubbles; D010743:Phospholipids / Q000008:administration & dosage* / Q000737:chemistry; D013459:Sulfur Hexafluoride / Q000008:administration & dosage* / Q000737:chemistry; D014465:Ultrasonics / Q000379:methods*
chemical_list: D000970:Antineoplastic Agents; D003287:Contrast Media; D010743:Phospholipids; C420843:contrast agent BR1; D001761:Bleomycin; D013459:Sulfur Hexafluoride