title: Gene cloning and function analysis of cytokine-induced suppressor of cytokine signaling (SOCS) from pearl oyster Pinctada fucata.
abstract: Cytokine-induced suppressor of cytokine signaling (SOCS) family acts as a negative regulator of cytokine receptor signaling to control excessive cytokine effects and inhibit a variety of signal transduction pathways, particularly the Janus kinases/signal transducers and activators of transcription (JAK/STAT) pathway. In present study, SOCS-2 homolog (PfSOCS-2) from pearl oyster Pinctada fucata was cloned and its gene has no intron. Multiple sequence alignments and phylogenetic analysis showed that PfSOCS-2 was clustered with other mollusk SOCS-2. LPS or polyI:C challenge and gene expression analysis revealed that PfSOCS-2 involved the innate immune response against bacterial and viral infections and that induction of PfSOCS-2 was varied with the different challenge stimulations. Furthermore, Dual-luciferase reporter assays showed that PfSOCS-2 involved in the regulation of vertebrate target genes containing the IFN-stimulated response element or NF-κB binding site in vitro. These results indicated that SOCS-2 from P. fucata plays a regulatory role against the stimulation.
authors: Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China.
authors: Wen-Guang
authors: WG
authors: Liu
authors: Xian-De
authors: XD
authors: Huang
authors: Qi
authors: Q
authors: Wang
authors: Mi
authors: M
authors: Zhao
authors: Shan-Zeng
authors: SZ
authors: Wu
authors: Mao-Xian
authors: MX
authors: He
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D020869:Gene Expression Profiling / Q000662:veterinary; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D049911:Pinctada / Q000235:genetics* / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D011070:Poly I-C / Q000008:administration & dosage; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012333:RNA, Messenger / Q000032:analysis; D016415:Sequence Alignment / Q000662:veterinary; D050826:Suppressor of Cytokine Signaling Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D050826:Suppressor of Cytokine Signaling Proteins; D011070:Poly I-C