title: Generation of  induced pluripotent mouse stem cells in an indirect co-culture system.
abstract: Typically,  production of induced pluripotent stem cells requires direct contact with  feeder cells. However, once the stem cells have reached the appropriate  maturation point, it is difficult to separate them from feeder cells, which  must be irradiated with γ-rays  or treated with the antibiotic mitomycin-C. We used a microporous  poly-membrane-based indirect contact co-culture system with mouse embryonic  fibroblasts to induce mouse pluripotent stem cells without radiation or  antibiotics. We found that induced pluripotent stem cells induced by this  co-culture method had a reprogramming efficiency and time similar to those  induced using traditional methods. Furthermore, strongly expressed pluripotent  markers showed a normal karyotype and formation and contained all three germ  layers in a teratoma.
authors: Department of Animal Breeding and Genetics, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors: F L
authors: FL
authors: Dong
authors: H A
authors: HA
authors: Kaleri
authors: Y D
authors: YD
authors: Lu
authors: C L
authors: CL
authors: Song
authors: B C
authors: BC
authors: Jiang
authors: B L
authors: BL
authors: Zhang
authors: L J
authors: LJ
authors: Wang
authors: X G
authors: XG
authors: Wang
authors: X S
authors: XS
authors: Ma
authors: B J
authors: BJ
authors: Wu
authors: H
authors: H
authors: Song
authors: J
authors: J
authors: Li
authors: H L
authors: HL
authors: Liu
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D002454:Cell Differentiation; D018920:Coculture Techniques / Q000295:instrumentation / Q000379:methods; D053595:Embryonic Stem Cells / Q000502:physiology*; D061252:Feeder Cells; D005347:Fibroblasts / Q000502:physiology; D018398:Homeodomain Proteins / Q000378:metabolism; D057026:Induced Pluripotent Stem Cells / Q000502:physiology* / Q000637:transplantation; D059785:Karyotype; D051379:Mice; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D000071317:Nanog Homeobox Protein; D013724:Teratoma / Q000473:pathology; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D000943:Antigens, Differentiation; D018398:Homeodomain Proteins; D000071317:Nanog Homeobox Protein; C475540:Nanog protein, mouse; C513672:Zfp42 protein, mouse; D014157:Transcription Factors