title: A preliminary comparison of the effect of 0.3% versus 0.2% chlorhexidine mouth rinse on de novo plaque formation: a monocentre randomized double-blind crossover trial.
abstract: OBJECTIVE
  Chlorhexidine (CHX) is considered the gold standard against gram-negative microorganisms. Little has been written about the simultaneous influence that both time and concentration could have on antiplaque formation effectiveness of CHX. The aim of this study is to compare the clinical and microbiological effectiveness of two different CHX concentrations and time applications in a 4-day plaque regrowth study model.
  
  
  METHODS
  Twenty volunteers were enrolled in a randomized double-blind crossover study comparing the effectiveness of CHX 0.3% and CHX 0.2% mouth rinses applied for 15 and 30 s, respectively. Plaque index (PII), total bacterial counts and the detection of specific periopathogens were measured at the 5th day of each mouth rinse application. Taste acceptance was evaluated using a questionnaire.
  
  
  RESULTS
  Chlorhexidine 0.3% resulted in a statistically greater reductions (10%) in PIl and periopathogens compared to CHX 0.2%. Furthermore, patients reported comparable taste acceptance in both groups.
  
  
  CONCLUSIONS
  Chlorhexidine is an effective oral antiseptic. The CHX 0.3% mouth rinse formulation used for 15 s resulted in superior clinical and microbiological outcomes compared to the CHX 0.2% formulation, used for 30 s.
authors: Department of Dentistry and Maxillofacial Surgery, Section of Periodontics, School of Dentistry, Sapienza University of Rome, Rome, Italy.
authors: A
authors: A
authors: Pilloni
authors: B
authors: B
authors: Zeza
authors: C
authors: C
authors: Mongardini
authors: F
authors: F
authors: Dominici
authors: M A
authors: MA
authors: Cassini
authors: A
authors: A
authors: Polimeni
mesh_terms: D000704:Analysis of Variance; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage*; D002710:Chlorhexidine / Q000008:administration & dosage*; D015169:Colony Count, Microbial; D018592:Cross-Over Studies; D003773:Dental Plaque / Q000517:prevention & control*; D003774:Dental Plaque Index; D004305:Dose-Response Relationship, Drug; D004311:Double-Blind Method; D006801:Humans; D009067:Mouthwashes / Q000008:administration & dosage*; D018709:Statistics, Nonparametric; D011795:Surveys and Questionnaires; D013649:Taste
chemical_list: D000891:Anti-Infective Agents, Local; D009067:Mouthwashes; D002710:Chlorhexidine
keywords: chlorhexidine; gingivitis; oral antiseptics; plaque formation