title: Effect of low-intensity focused ultrasound on the middle ear in a mouse model of acute otitis media.
abstract: We hypothesized that low-intensity focused ultrasound (LIFU) increases vessel permeability and antibacterial drug activity in the mouse middle ear. We determined appropriate settings by applying LIFU to mouse ears with the external auditory canal filled with normal saline and performed histologic and immunohistologic examination. Acute otitis media was induced in mice with nontypable Haemophilus influenzae, and they were given ampicillin (50, 10, or 2 mg/kg) intraperitoneally once daily for 3 days with or without LIFU (1.0 W/cm(2), 20% duty cycle, 30 s). In the LIFU(+) groups receiving the 2- and 10-mg/kg doses, viable bacteria counts, number of inflammatory cells and IL-1β and TNF-α levels in middle ear effusion were significantly lower than in the LIFU(-) groups on the same doses. Severity of AOM also tended to be reduced more in the LIFU(+) groups than in the LIFU(-) groups. LIFU application with antibiotics may be effective for middle ear infection.
authors: Department of Otolaryngology, Oita University Faculty of Medicine, Oita, Japan.
authors: Kanako
authors: K
authors: Noda
authors: Takashi
authors: T
authors: Hirano
authors: Kenji
authors: K
authors: Noda
authors: Satoru
authors: S
authors: Kodama
authors: Issei
authors: I
authors: Ichimiya
authors: Masashi
authors: M
authors: Suzuki
mesh_terms: D000208:Acute Disease; D000667:Ampicillin / Q000008:administration & dosage; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D058491:Bacterial Load / Q000187:drug effects; D004195:Disease Models, Animal; D004432:Ear, Middle / Q000382:microbiology; D006192:Haemophilus Infections / Q000382:microbiology / Q000628:therapy; D006193:Haemophilus influenzae / Q000187:drug effects; D053583:Interleukin-1beta / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010033:Otitis Media / Q000382:microbiology / Q000628:therapy*; D010034:Otitis Media with Effusion / Q000382:microbiology / Q000628:therapy; D010446:Peptide Fragments / Q000187:drug effects; D012720:Severity of Illness Index; D012965:Sodium Chloride; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects; D014464:Ultrasonic Therapy / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D053583:Interleukin-1beta; D010446:Peptide Fragments; D014409:Tumor Necrosis Factor-alpha; C073692:interleukin-1beta (204-215); D012965:Sodium Chloride; D000667:Ampicillin