title: Anticancer efficacy of a novel propofol-linoleic acid-loaded escheriosomal formulation against murine hepatocellular carcinoma.
abstract: OBJECTIVE
  The preparation and characterization of a novel escheriosomal nanoparticle formulation of a potent anticancer conjugate, 2,6-diisopropylphenol-linoleic acid (2,6P-LA), and evaluation of its anticancer efficacy against diethyl nitrosamine-induced hepatocellular carcinoma (HCC) in BALB/c mice.
  
  
  METHODS
  Escheriosomized 2,6P-LA nanoparticles were characterized for size, zeta-potential, entrapment efficiency, release kinetics and in vivo toxicity. Their anticancer potential was evaluated on the basis of survival, DNA fragmentation, caspase-3 activation, western blot analysis of apoptotic factors and histopathological changes in hepatocytes of treated animals.
  
  
  RESULTS
  The escheriosomized 2,6P-LA nanoparticles exhibited low toxicity, biocompatibility and bioavailability. As revealed by apoptosis induction, survival rate, expression profiles of Bax, Bcl-2 and caspase-9, escheriosomized 2,6P-LA nanoparticles were more effective in the treatment of HCC than the free form of 2,6P-LA in experimental animals.
  
  
  CONCLUSIONS
  2,6P-LA-bearing escheriosome nanoparticles are effective in suppressing HCC in mice. Original submitted 17 January 2012; Revised submitted 27 August 2012; Published online 14 January 2013.
authors: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India. azmatbiotech@gmail.com
authors: Azmat Ali
authors: AA
authors: Khan
authors: Mumtaz
authors: M
authors: Jabeen
authors: Aijaz Ahmed
authors: AA
authors: Khan
authors: Mohammad
authors: M
authors: Owais
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000473:pathology; D053148:Caspase 3 / Q000096:biosynthesis; D053938:DNA Fragmentation / Q000187:drug effects; D006801:Humans; D019787:Linoleic Acid / Q000008:administration & dosage / Q000737:chemistry*; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D015742:Propofol / Q000008:administration & dosage* / Q000737:chemistry; D016019:Survival Analysis
chemical_list: D019787:Linoleic Acid; D053148:Caspase 3; D015742:Propofol