title: Surfactant protein-A concentration in sera from dogs with pulmonary parenchymal diseases.
abstract: Pulmonary surfactant protein A (SP-A) is used as a biomarker to understand the clinical features of pulmonary diseases and associated prognostic indices in human medicine. This study was conducted to investigate whether or not serum SP-A concentration can be used as a biomarker for identifying pulmonary parenchymal diseases in dogs. Thirty-two dogs with pulmonary parenchymal diseases, 34 with nonrespiratory diseases and 57 healthy dogs were included. Serum SP-A concentration was measured in all dogs using sandwich enzyme linked immunosorbent assay with an anti-dog SP-A polyclonal antibody. Median serum SP-A concentration in healthy dogs was <2.0 ng/ml, whereas that in dogs with aspiration pneumonia (n=11), primary lung tumors (n=9) and blunt traumatic lung injury (BTLI; n=12) was 3.1, 7.2 and 2.6 ng/ml, respectively; these values were significantly higher than those in healthy dogs. The serum SP-A concentration in dogs with nonrespiratory diseases was comparable with that in healthy dogs. No correlation was observed between the serum SP-A and plasma C-reactive protein concentrations in dogs with aspiration pneumonia and BTLI. There was a significant correlation between the serum SP-A concentration and thoracic radiographic changes in dogs with BTLI. These findings suggest that the serum SP-A concentration may be a useful clinical biomarker of alveolar damage that can be used for differential diagnosis of pulmonary parenchymal diseases and nonrespiratory diseases in dogs.
authors: Department of Veterinary Clinical Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-ohrai-kita, Izumisano, Osaka 598-8531, Japan. k-sone@land222.com
authors: Katsuhito
authors: K
authors: Sone
authors: Hideo
authors: H
authors: Akiyoshi
authors: Junichiro
authors: J
authors: Shimizu
authors: Zheng
authors: Z
authors: Cao
authors: Yijyun
authors: Y
authors: Li
authors: Toshiyuki
authors: T
authors: Tanaka
authors: Akiyoshi
authors: A
authors: Hayashi
authors: Shunji
authors: S
authors: Sugii
authors: Fumihito
authors: F
authors: Ohashi
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D004283:Dog Diseases / Q000097:blood* / Q000378:metabolism; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D008171:Lung Diseases / Q000097:blood / Q000378:metabolism / Q000662:veterinary*; D008297:Male; D037662:Pulmonary Surfactant-Associated Protein A / Q000097:blood* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D037662:Pulmonary Surfactant-Associated Protein A