title: Differences in transcription patterns between induced pluripotent stem cells produced from the same germ layer are erased upon differentiation.
abstract: Little is known about differences between induced pluripotent stem cells produced from tissues originating from the same germ layer. We have generated human myoblast-derived iPS cells by retroviral transduction of human primary myoblasts with the OCT3/4, SOX2, KLF4 and MYC coding sequences and compared them to iPS produced from human primary fibroblasts. When cultivated in vitro, these iPS cells proved similar to human embryonic stem cells in terms of morphology, expression of embryonic stemness markers and gene promoter methylation patterns. Embryonic bodies were derived that expressed endodermal, mesodermal as well as ectodermal markers. A comparative analysis of transcription patterns revealed significant differences in the gene expression pattern between myoblast- and fibroblast-derived iPS cells. However, these differences were reduced in the mesenchymal stem cells derived from the two iPS cell types were compared.
authors: CNRS, Université Paris-Sud, UMR-8126, Institut de cancérologie Gustave Roussy, Villejuif, France.
authors: Iryna
authors: I
authors: Pirozhkova
authors: Ana
authors: A
authors: Barat
authors: Petr
authors: P
authors: Dmitriev
authors: Elena
authors: E
authors: Kim
authors: Thomas
authors: T
authors: Robert
authors: Justine
authors: J
authors: Guégan
authors: Chrystèle
authors: C
authors: Bilhou-Nabera
authors: Florence
authors: F
authors: Busato
authors: Jörg
authors: J
authors: Tost
authors: Gilles
authors: G
authors: Carnac
authors: Dalila
authors: D
authors: Laoudj-Chenivesse
authors: Marc
authors: M
authors: Lipinski
authors: Yegor
authors: Y
authors: Vassetzky
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002478:Cells, Cultured; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D020869:Gene Expression Profiling; D005855:Germ Layers / Q000166:cytology / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D024510:Muscle Development / Q000235:genetics; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism*; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D012190:Retroviridae / Q000235:genetics; D055748:SOXB1 Transcription Factors / Q000235:genetics; D014161:Transduction, Genetic
chemical_list: C000715467:KLF4 protein, human; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; C489427:MYC protein, human; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D016271:Proto-Oncogene Proteins c-myc; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors