title: Comparison of modified ultrafast Papanicolaou stain with the standard rapid Papanicolaou stain in cytology of various organs.
abstract: BACKGROUND
  Since the first introduction of Papanicolaou (Pap) stain in 1942 there have been many modifications. Of these, the Ultra-Fast Pap stain has become popular. This technique was further modified in India as many of the reagents were not available in our country. Our study was conducted by adapting this modified staining technique which involves the replacement of Gill's hematoxylin with Harris hematoxylin.
  
  
  OBJECTIVE
  The aim of our prospective study was to assess the use of the modified Ultra-Fast Pap stain (MUFP) for fine needle aspiration cytology (FNAC) of various organs in comparison with the standard rapid Pap stain.
  
  
  METHODS
  A total of 100 FNAC cases were studied by random sampling. Two smears were prepared for each case and stained by both, the MUFP and the rapid Pap stain. Scores were given and the quality index was calculated, followed by the statistical analysis. The number of cases was as follows: lymph node (43), thyroid (25), breast (23), salivary gland (02), and soft tissues (07). Scores were given on four parameters: Background of smears, overall staining pattern, cell morphology and nuclear staining. Quality index was calculated from the ratio of score achieved to the maximum score possible.
  
  
  METHODS
  Results were analyzed using Mean, Median, Standard Deviation, 't' paired test, 'P' value and M-diff for statistical significance.
  
  
  RESULTS
  Correct diagnosis was made in all cases. The quality index of MUFP smears was better compared to the rapid Pap stain in all the organs, and was statistically significant. MUFP smears showed a clear red blood cells background, transparent cytoplasm and crisp nuclear features.
  
  
  CONCLUSIONS
  MUFP is a reliable and rapid technique for cytology diagnosis.
authors: Department of Pathology, Dr. D.Y. Patil Medical College, Hospital and Research Center, Nerul, Navi Mumbai, India.
authors: Priyanka
authors: P
authors: Choudhary
authors: S
authors: S
authors: Sudhamani
authors: Ajita
authors: A
authors: Pandit
authors: Vm
authors: V
authors: Kiri
keywords: Fine needle aspiration cytology; modified Pap stain; papanicolaou stain; rapid Pap stain; ultra-fast Pap stain