title: Edaphobacillus lindanitolerans gen. nov., sp. nov., isolated from hexachlorocyclohexane (HCH) contaminated soil.
abstract: A Gram-staining-positive, aerobic, cocco-bacilli-shaped, non-motile, non-spore forming, cream colored strain bacterium (strain MNA4(T) ) was isolated from hexachlorocyclohexane (HCH) contaminated soil. Strain MNA4(T) showed the highest 16S rRNA gene sequence similarity of 97.47% with type species of the newly defined genus Bhargavaea cecembensis. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MNA4(T) belonged to a clade represented by Bhargavaea cecembensis, Bacillus beijingensis and Bacillus ginsengi. DNA-DNA hybridization values of the strain MNA4(T) with close relatives were well below the 70% threshold value recommended for delineation of species. The major fatty acids were anteiso C15:0 , anteiso C17:0 , iso C16:0 and iso C15:0 . The strain was found to contain respiratory quinones MK-8, MK-9 and MK-7 in an approximate molar ratio of 85:7:5. Polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol and two unknown phospholipids. The DNA G + C content was 55.6 mol%. Peptidoglycan type was A4α (L-Lys - L-Ala - D-Asp). Phylogenetic analysis, fatty acids profile, phenotypic properties and chemotaxonomic data of strain MNA4(T) indicated that it represents a novel species of a novel genus for which the name Edaphobacillus lindanitolerans gen. nov., sp. nov., is proposed. The type strain is MNA4(T) (=CCM 7584(T)  = DSM 22424(T) ).
authors: Department of Zoology, Molecular Biology Laboratory, University of Delhi, Delhi, India.
authors: Devi
authors: D
authors: Lal
authors: Fazlurrahman
authors: F
authors: Khan
authors: Sanjay Kumar
authors: SK
authors: Gupta
authors: Peter
authors: P
authors: Schumann
authors: Rup
authors: R
authors: Lal
mesh_terms: D056449:Bacillales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D001556:Hexachlorocyclohexane / Q000378:metabolism*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D001556:Hexachlorocyclohexane
keywords: Bacilliaceae; Contaminated soil; Edaphobacillus lindanitolerans; Hexachlorocyclohexane; Insecticide