title: A new nanobiocatalytic system based on allosteric effect with dramatically enhanced enzymatic performance.
abstract: We report a rational design of CaHPO(4)-α-amylase hybrid nanobiocatalytic system based on allosteric effect and an explanation of the increase in catalytic activity when certain enzymes are immobilized in specific nanomaterials. Employing a calcification approach in aqueous solutions, we acquired such new nanobiocatalytic systems with three different morphologies, i.e., nanoflowers, nanoplates, and parallel hexahedrons. Through studying enzymatic performance of these systems and free α-amylase with/without Ca(2+), we demonstrated how two factors, allosteric regulation and morphology of the as-synthesized nanostructures, predominantly influence enzymatic activity. Benefiting from both the allosteric modulation and its hierarchical structure, CaHPO(4)-α-amylase hybrid nanoflowers exhibited dramatically enhanced enzymatic activity. As a bonus, the new system we devised was found to enjoy higher stability and durability than free α-amylase plus Ca(2+).
authors: Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, Anhui 230026, PR China.
authors: Liang-Bing
authors: LB
authors: Wang
authors: You-Cheng
authors: YC
authors: Wang
authors: Rong
authors: R
authors: He
authors: Awei
authors: A
authors: Zhuang
authors: Xiaoping
authors: X
authors: Wang
authors: Jie
authors: J
authors: Zeng
authors: J G
authors: JG
authors: Hou
mesh_terms: D000494:Allosteric Regulation; D000681:Amylases / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D002130:Calcium Phosphates / Q000737:chemistry / Q000378:metabolism*; D036103:Nanotechnology; D009942:Organometallic Compounds / Q000737:chemistry / Q000378:metabolism*; D010316:Particle Size; D013499:Surface Properties
chemical_list: D002130:Calcium Phosphates; D009942:Organometallic Compounds; C485838:calcium phosphate, monobasic, anhydrous; D000681:Amylases