title: Application of microwave irradiation to fast and efficient isolation of benzodiazepines from human hair.
abstract: The aim of this research was to develop, optimize and validate a modern and rapid method of preparation of human hair samples using microwave irradiation for the purpose of determination of six benzodiazepines: alprazolam, estazolam, lorazepam, clonazepam, diazepam and tetrazepam, with lormetazepam as an internal standard (IS). Extracts were analyzed ultrahigh performance liquid chromatography with mass spectrometry time of flight detection (UHPLC-MS-TOF). Optimal parameters of microwave-assisted extraction (type of extraction solvent, extraction temperature, extraction time and pH of borate buffer) for these drugs in spiked hair samples were defined. The limit of detection ((0.003-0.025 ng/mg), intra- (1.5-4.3%) and interday (2.3-8.3%) precision (CV) and accuracy of the assay (89.8-105.7%) at three concentration levels: 0.22, 2.22 and 5.56 ng/mg were calculated. The optimized and validated MAE/UHPLC-MS-TOF method was then applied for analysis of clinical hair sample.
authors: Laboratory for Forensic Chemistry, Department of Analytical Chemistry, Jagiellonian University, 3 Ingardena St., 30-060 Kraków, Poland. renata.wietecha-posluszny@uj.edu.pl
authors: Renata
authors: R
authors: Wietecha-Posłuszny
authors: Michał
authors: M
authors: Woźniakiewicz
authors: Aneta
authors: A
authors: Garbacik
authors: Paulina
authors: P
authors: Chęsy
authors: Paweł
authors: P
authors: Kościelniak
mesh_terms: D000328:Adult; D001569:Benzodiazepines / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D006197:Hair / Q000737:chemistry*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008872:Microwaves; D012015:Reference Standards
chemical_list: D001569:Benzodiazepines