title: Genetic diversity of Tomistoma schlegelii inferred from mtDNA markers.
abstract: The genetic diversity of the endangered crocodile Tomistoma schlegelii was characterized using the protein coding ND 6-tRNA(glu)-cyt b and the cytochrome b-control region (cyt b-CR) markers. Concatenate data revealed six haplotypes with an overall haplotype diversity of 0.769 ± 0.039; nucleotide diversity was 0.00535 ± 0.00172. A nearest-neighbor analysis showed that all individuals clustered with four geographic regions (Sumatra, Peninsular Malaysia, Sarawak, and East Kalimantan) and were genetically differentiated. With the exception of the individuals from haplotype H2, which occurred in both Peninsular Malaysia and Sarawak, all other haplotypes were geographically distinct. The H4 lineage, which was found to be the most divergent, clustered exclusively in the basal clade in all phylogenetic trees, and the haplotype network was unconnected at the 95% reconnection limit, suggesting further investigation to establish its possible status as a distinct evolutionary significant unit or a cryptic species.
authors: Faculty of Engineering and Science, Universiti Tunku Abdul Rahman, 53300, Kuala Lumpur, Malaysia.
authors: Taranjeet
authors: T
authors: Kaur
authors: Jeffrine Rovie Ryan
authors: JR
authors: Japning
authors: Mohamad Shahbudin
authors: MS
authors: Sabki
authors: Irvan
authors: I
authors: Sidik
authors: Lee Kim
authors: LK
authors: Chong
authors: Alan H K
authors: AH
authors: Ong
mesh_terms: D000489:Alligators and Crocodiles / Q000235:genetics*; D000818:Animals; D045303:Cytochromes b / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D056727:Endangered Species; D005819:Genetic Markers; D014644:Genetic Variation; D006723:Hong Kong; D007214:Indonesia; D019901:Locus Control Region / Q000235:genetics; D008296:Malaysia; D012846:Singapore
chemical_list: D004272:DNA, Mitochondrial; D005819:Genetic Markers; D045303:Cytochromes b