title: Identification and characterization of PtDGAT2B, an acyltransferase of the DGAT2 acyl-coenzyme A: diacylglycerol acyltransferase family in the diatom Phaeodactylum tricornutum.
abstract: Diacylglycerol acyltransferase (DGAT) plays a pivotal role in triacylglycerol (TAG) formation in some oleaginous organisms. We describe here the identification of a type 2 DGAT (PtDGAT2B) in the diatom Phaeodactylum tricornutum that contains four putative type 2 acyl-CoA:DGATs, sharing little sequence similarity with each other. TAG synthesis and lipid body formation could be completely restored in a Saccharomyces cerevisiae TAG-deficient quadruple mutant by expressing PtDGAT2B. Up-regulation of PtDGAT2B precedes the accumulation of TAG. Functional analysis of enzyme activity in vivo demonstrated that expression of PtDGAT2B can increase the proportion of unsaturated C(16) and C(18) fatty acids in yeast TAG.
authors: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, PR China.
authors: Yangmin
authors: Y
authors: Gong
authors: Junping
authors: J
authors: Zhang
authors: Xiaojing
authors: X
authors: Guo
authors: Xia
authors: X
authors: Wan
authors: Zhuo
authors: Z
authors: Liang
authors: Chuan Jiong
authors: CJ
authors: Hu
authors: Mulan
authors: M
authors: Jiang
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D017124:Conserved Sequence; D051048:Diacylglycerol O-Acyltransferase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D017377:Diatoms / Q000201:enzymology* / Q000378:metabolism; D015870:Gene Expression; D008969:Molecular Sequence Data; D010802:Phylogeny; D010843:Pichia; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D013379:Substrate Specificity; D014280:Triglycerides / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D014280:Triglycerides; D051048:Diacylglycerol O-Acyltransferase