title: FAM172A induces S phase arrest of HepG2 cells via Notch 3.
abstract: Our previous results revealed that FAM172A was significantly downregulated             in liver tissue from hepatocellular carcinoma or cirrhotic patients. The present             study was designed to elucidate the regulatory role of FAM172A in HepG2 cells.             In order to determine the expression of the FAM172A protein, western blot analysis             was performed. Confocal laser scanning technique was used to observe the localization             of FAM172A in HepG2 cells. Surface plasmon resonance experiments were used to             determine the binding activity of FAM172A and active single sugar and Ca2+. The             cell cycle progression of HepG2 cells was assessed by flow cytometry. The FAM172A             protein was localized in the endoplasmic reticulum of HepG2 cells. This protein             was moderately expressed in normal liver tissue, but was significantly decreased             in liver tissue of patients with chronic hepatitis B When co-cultured with the             FAM172A recombinant protein, HepG2 cells exhibited complete cell cycle arrest             in the S phase at a high concentration (100 ng/ml). Proliferation of HepG2 cells             treated with the FAM172A recombinant protein was prominently inhibited compared             with that of the control cells. Western blot analysis showed that upregulation             of Notch 3 and cyclin E may be related with the cell cycle control. Our results             indicate that FAM172A may be a novel tumor-suppressor gene, which plays an important             role in cell cycle control and tumor cell proliferation. G1/S phase arrest may             be mediated, at least partially, by the Notch 3 signaling pathway.
authors: Department of Hepatobiliary Surgery, Air Force General Hospital of PLA, Beijing 100142, PR China.
authors: Zhiqiang
authors: Z
authors: Feng
authors: Hongqi
authors: H
authors: Li
authors: Shunai
authors: S
authors: Liu
authors: Jun
authors: J
authors: Cheng
authors: Guoan
authors: G
authors: Xiang
authors: Jinqian
authors: J
authors: Zhang
mesh_terms: D002118:Calcium / Q000737:chemistry; D049109:Cell Proliferation; D019927:Cyclin E / Q000378:metabolism; D015536:Down-Regulation; D004721:Endoplasmic Reticulum / Q000378:metabolism; D059865:Endoplasmic Reticulum Stress; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver / Q000378:metabolism; D011485:Protein Binding; D021381:Protein Transport; D011506:Proteins / Q000737:chemistry / Q000502:physiology*; D000071656:Receptor, Notch3; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism*; D059807:S Phase Cell Cycle Checkpoints
chemical_list: D002118:Calcium; D019927:Cyclin E; D011506:Proteins; D000071656:Receptor, Notch3; D051880:Receptors, Notch; C550020:ARB2A protein, human; C499374:NOTCH3 protein, human