title: Evaluation of bone substitute materials: comparison of flat-panel based volume CT to conventional multidetector CT.
abstract: Over the last decade tissue engineering has emerged as a key factor in bone regeneration within the field of cranio-maxillofacial surgery. Despite this in vivo analysis of tissue-engineered-constructs to monitor bone rehabilitation are difficult to conduct. Novel high-resolving flat-panel based volume CTs (fp-VCT) are increasingly used for imaging bone structures. This study compares the potential value of novel fp-VCT with conventional multidetector CT (MDCT) based on a sheep sinus floor elevation model. Calcium-hydroxyapatite reinforced collagen scaffolds were populated with autologous osteoblasts and implanted into sheep maxillary sinus. After 8, 16 and 24 weeks MDCT and fp-VCT scans were performed to investigate the volume of the augmented area; densities of cancellous and compact bone were assessed as comparative values. fp-VCT imaging resulted in higher spatial resolution, which was advantageous when separating closely related anatomical structures (i.e. trabecular and compact bone, biomaterials). Fp-VCT facilitated imaging of alterations occurring in test specimens over time. fp-VCTs therefore displayed high volume coverage, dynamic imaging potential and superior performance when investigating superfine bone structures and bone remodelling of biomaterials. Thus, fp-VCTs may be a suitable instrument for intraoperative imaging and future in vivo tissue-engineering studies.
authors: Department of Oral and Craniomaxillofacial Surgery, University Hospital Freiburg, Hugstetter Str. 55, D-79106 Freiburg, Germany.
authors: Sebastian
authors: S
authors: Sauerbier
authors: Fabian
authors: F
authors: Duttenhoefer
authors: Elefterios
authors: E
authors: Sachlos
authors: Jörg
authors: J
authors: Haberstroh
authors: Christian
authors: C
authors: Scheifele
authors: Karl-Thomas
authors: KT
authors: Wrbas
authors: Pit Jacob
authors: PJ
authors: Voss
authors: Egle
authors: E
authors: Veigel
authors: Jörg
authors: J
authors: Smedek
authors: Philip
authors: P
authors: Ganter
authors: Taskin
authors: T
authors: Tuna
authors: Ralf
authors: R
authors: Gutwald
authors: Moritz
authors: M
authors: Palmowski
mesh_terms: D000818:Animals; D001031:Apatites / Q000737:chemistry; D064592:Autografts / Q000637:transplantation; D015519:Bone Density / Q000502:physiology; D016723:Bone Remodeling / Q000502:physiology; D018786:Bone Substitutes / Q000737:chemistry*; D018929:Cell Culture Techniques; D003094:Collagen / Q000737:chemistry; D004338:Drug Combinations; D007091:Image Processing, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods; D008443:Maxillary Sinus / Q000000981:diagnostic imaging / Q000601:surgery; D061330:Multidetector Computed Tomography / Q000295:instrumentation / Q000379:methods*; D010006:Osteoblasts / Q000637:transplantation; D011897:Random Allocation; D012756:Sheep; D059546:Sinus Floor Augmentation / Q000379:methods*; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry; D014057:Tomography, X-Ray Computed / Q000295:instrumentation / Q000379:methods*
chemical_list: D001031:Apatites; D018786:Bone Substitutes; D004338:Drug Combinations; C040699:calcium hydroxyapatite, collagen drug combination; D003094:Collagen
keywords: Bone substitute materials; Flat-panel CT; In vivo imaging; Tissue engineering