title: Fecal shedding in cattle inoculated with Escherichia coli O157:H7 and fed corn or wheat distillers' dried grain with solubles.
abstract: Feeding corn dried distillers' grain with solubles (DDGS) has been linked to increased fecal shedding of Escherichia coli O157:H7 in cattle. A study was conducted to compare the impact of three diets containing (dry matter basis) 40% corn DDGS, 40% wheat DDGS, or 20% corn and  20% wheat mixed DDGS to a standard barley grain finishing diet on fecal shedding in cattle challenged with a 10(10) CFU mixture of four nalidixic acid-resistant E. coli O157:H7 strains. Rectal grab samples (n = 544) were collected over 70 days and screened for  E. coli O157:H7 by direct plating and immunomagnetic bead separation. Feeding diets containing DDGS had no effect (P > 0.05) on the intensity or duration of fecal shedding of E. coli O157:H7 compared with the standard barley grain finishing diet.
authors: Department of Biological Sciences, University of Lethbridge, 4401 University Drive, Lethbridge, Alberta, Canada T1K 3M4.
authors: J
authors: J
authors: Hallewell
authors: L R
authors: LR
authors: Barbieri
authors: J E
authors: JE
authors: Thomas
authors: K
authors: K
authors: Stanford
authors: T A
authors: TA
authors: McAllister
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D057138:Bacterial Shedding; D002417:Cattle / Q000382:microbiology*; D002418:Cattle Diseases / Q000517:prevention & control; D004927:Escherichia coli Infections / Q000517:prevention & control / Q000662:veterinary; D019453:Escherichia coli O157 / Q000302:isolation & purification* / Q000502:physiology; D005243:Feces / Q000382:microbiology*; D005285:Fermentation; D001467:Hordeum / Q000382:microbiology; D050296:Microbial Viability; D011634:Public Health; D014908:Triticum / Q000382:microbiology