title: Structural characterization of minor metabolites and pharmacokinetics of ganoderic acid C2 in rat plasma by HPLC coupled with electrospray ionization tandem mass spectrometry.
abstract: The metabolites and pharmacokinetics of ganoderic acid C2 (GAC2), a bioactive triterpenoid in Ganoderma lucidum in rat plasma were investigated by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). Totally, ten minor phase I metabolites of GAC2 were characterized after oral administration of GAC2, on the basis of their mass fragmentation pathways or direct comparison with authentic compounds by high-performance liquid chromatography coupled with diode array detection and electrospray ion trap tandem mass spectrometry (HPLC-DAD-ESI-MS(n)), and liquid chromatography coupled with electrospray ionization hybrid ion trap and time-of-flight mass spectrometry (LC-ESI-IT-TOF/MS) methods. Moreover, a rapid and specific method for quantification of GAC2 in rat plasma after oral administration was developed by using a liquid-liquid extraction procedure and HPLC-ESI-MS/MS analysis. It is the first time to report the metabolites and pharmacokinetics of GAC2.
authors: Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
authors: Xiao-Yu
authors: XY
authors: Guo
authors: Dan
authors: D
authors: Liu
authors: Min
authors: M
authors: Ye
authors: Jian
authors: J
authors: Han
authors: Sa
authors: S
authors: Deng
authors: Xiao-Chi
authors: XC
authors: Ma
authors: YanYan
authors: Y
authors: Zhao
authors: Baojing
authors: B
authors: Zhang
authors: Xuan
authors: X
authors: Shen
authors: Qing-Ming
authors: QM
authors: Che
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D001682:Biological Availability; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D006207:Half-Life; D057230:Limit of Detection; D059625:Liquid-Liquid Extraction; D008297:Male; D050216:Metabolic Detoxication, Phase I; D015394:Molecular Structure; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D014315:Triterpenes / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D014315:Triterpenes; C557338:ganoderic acid C2