title: Influence of Trueperella pyogenes in uterus on corpus luteum lifespan in cycling cows.
abstract: To study ovarian responses to long-term intrauterine infusions of Trueperella pyogenes (T. pyogenes), 12 nonlacting Holstein cows were transcervically infused with 10 mL of a bacterial solution (8-19 × 10(8) colony-forming units/mL), and the uteri of another four cows (control) were similarly infused with sterile physiological saline. Infusions were done six times, every 3 days from Days 3 to 18 (Day 0 = day of spontaneous ovulation). Development of ovarian follicles and the CL were monitored with transrectal, real-time ultrasonography. In five of the experimentally infected cows (group A), the CL, which developed after Day 0, regressed without maturing, and the first dominant follicle (DF) ovulated (mean ± SEM interovulatory interval, 8.6 ± 0.5 days). In group A, plasma 13,14-dihydro-15-keto-PGF(2α) (PGFM) concentrations rose sharply on Day 6, but plasma progesterone concentrations did not increase substantially (as in the control) and were maintained at approximately 2.5 ng/mL after the first DF ovulated. In seven of the 12 infected cows (group B), the developing CL which formed after Day 0 matured and the second DF ovulated. However, the CL lifespan was shorter (P < 0.01) and the second DF ovulated earlier than in control (interovulatory interval, 16.0 ± 0.4 days and 22.3 ± 1.9 days; P < 0.01). Although there was no sharp increase in PGFM in group B, it tended to be high between Days 11 and 18. In conclusion, long-term, intrauterine infusions of T. pyogenes caused the CL to regress prematurely or to have a somewhat shorter lifespan because of release of endogenous PGF(2α).
authors: Department of Veterinary Obstetrics and Gynecology, Azabu University, Kanagawa, Japan. kaneko@azabu-u.ac.jp
authors: Kazuyuki
authors: K
authors: Kaneko
authors: Makoto
authors: M
authors: Nakamura
authors: Reiichiro
authors: R
authors: Sato
mesh_terms: D000191:Actinomycetaceae; D000193:Actinomycetales Infections / Q000150:complications / Q000000981:diagnostic imaging / Q000662:veterinary*; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000000981:diagnostic imaging / Q000382:microbiology*; D003338:Corpus Luteum / Q000000981:diagnostic imaging / Q000382:microbiology* / Q000473:pathology; D015237:Dinoprost / Q000031:analogs & derivatives / Q000097:blood; D030762:Estrous Cycle; D005260:Female; D010060:Ovulation / Q000502:physiology; D011374:Progesterone / Q000097:blood; D014463:Ultrasonography; D014591:Uterine Diseases / Q000150:complications / Q000000981:diagnostic imaging / Q000382:microbiology / Q000662:veterinary*; D014599:Uterus / Q000382:microbiology*
chemical_list: C008228:15-keto-13,14-dihydroprostaglandin F2alpha; D011374:Progesterone; D015237:Dinoprost