title: [Regulations of function and maturation of plasmacytoid dendritic cells from primary immune thrombocytopenia patients by triptolide].
abstract: OBJECTIVE
  To explore the mechanism of immunomodulatory activity of triptolide on primary immune thrombocytopenia (ITP)patients-derived plasmacytoid dendritic cells (pDCs).
  
  
  METHODS
  pDCs in peripheral blood of ITP patients before therapy (group 1), ITP patients in complete response (ITP-CR, group 2) and healthy donors (group 3) were sorted by flow cytometry, then incubated with triptolide at 0, 5, 10 or 30 µg/L. After 24 hours, we collected the supernatants and then detected the concentrations of IFN-α, IL-6 and TNF-α using ELISA. After 5 days, the cultured cells were collected and CD11c, CD80 and CD86 expressions of myeloid dendritic cells (mDCs) were analyzed by flow cytometry, the morphology of mDC was observed by light microscope and electron microscope.
  
  
  RESULTS
  After incubation with triptolide at 10 µg/L, the levels of IFN-α, IL-6 and TNF-α in group 1 \[(451.32 ± 85.77) ng/L, (105.68 ± 23.85) ng/L and (135.78 ± 30.62) ng/L\] and group 2 \[(391.71 ± 72.49) ng/L, (84.73 ± 17.77) ng/L and (108.16 ± 23.21) ng/L\] were significantly higher than those in group 3 \[(335.51 ± 67.54) ng/L, (73.62 ± 21.82) ng/L and (95.58 ± 32.85) ng/L\] (all P < 0.05); the levels of IFN-α, IL-6 and TNF-α in group 1 were significantly higher than those in group 2 (all P < 0.05) in a dose-dependent manner (P < 0.05). CD11c, CD80 and CD86 expressions of mDC in group1 and group 2 were significantly higher than those in group 3 (all P < 0.05); CD11c, CD80 and CD86 expressions of mDC in group 1 were significantly higher than those in group 2 (all P < 0.05) also in a dose-dependent manner (all P < 0.05). Triptolide could inhibit pDCs from differentiation into mDCs, the latter displayed more immature morphology than untreated-pDCs.
  
  
  CONCLUSIONS
  Triptolide could decrease the immune function of pDCs from ITP, inhibit pDCs from differentiation and maturation.
authors: Department of Hematology, Second People Hospital of Huaian City, Huaian, China.
authors: Li-Min
authors: LM
authors: Liu
authors: Xue-Hua
authors: XH
authors: Han
authors: Xing-Xia
authors: XX
authors: Zhang
authors: Guang-Sheng
authors: GS
authors: Zhao
authors: Ye-Jun
authors: YJ
authors: Si
authors: Guo-Qiang
authors: GQ
authors: Lin
authors: Yan-Ming
authors: YM
authors: Zhang
authors: Yu-Mei
authors: YM
authors: Sun
authors: Guang-Sheng
authors: GS
authors: He
authors: De-Pei
authors: DP
authors: Wu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects*; D004224:Diterpenes / Q000494:pharmacology*; D004852:Epoxy Compounds / Q000494:pharmacology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010616:Phenanthrenes / Q000494:pharmacology*; D013921:Thrombocytopenia / Q000209:etiology / Q000276:immunology; D055815:Young Adult
chemical_list: D004224:Diterpenes; D004852:Epoxy Compounds; D010616:Phenanthrenes; C001899:triptolide