title: Toxicity assessment on trophoblast cells for some environment polluting chemicals and 17β-estradiol.
abstract: The identification of reproductive toxicants is a major scientific challenge for human health. We investigated the effects of a selected group of environmental polluting chemicals mostly provided with estrogenic activity on the human trophoblast cell lines BeWo and HTR-8/SVneo. Cells were exposed for 24h to various concentrations (from 0.1 pM to 1 mM) of atrazine (ATR), diethylstilbestrol (DES), para-nonylphenol (p-NP), resveratrol (RES) and 17 β-estradiol (E2) and assayed for cell viability and human beta-Chorionic Gonadotropin (β-hCG) secretion. Decrease of cell viability as respect to control, vehicle-treated, cultures was obtained for all chemicals in the concentration range of 1 μM-1 mM in both cell types. A parallel decrease of β-hCG secretion was observed in BeWo cells, at 1 μM-1 mM concentrations, with the only exception of ATR which caused an increase at concentrations up to 1mM. β-hCG release was also unexpectedly inhibited by ATR, DES, p-NP and RES at non-toxic (pM-nM) concentrations. These findings raise concern about the negative, potential effects of various environmental polluting chemicals on pregnancy success and fetal health.
authors: San Carlo Clinic, Paderno Dugnano, Milano, Italy. bechi4@unisi.it
authors: N
authors: N
authors: Bechi
authors: G
authors: G
authors: Sorda
authors: A
authors: A
authors: Spagnoletti
authors: J
authors: J
authors: Bhattacharjee
authors: E A
authors: EA
authors: Vieira Ferro
authors: B
authors: B
authors: de Freitas Barbosa
authors: M
authors: M
authors: Frosini
authors: M
authors: M
authors: Valoti
authors: G
authors: G
authors: Sgaragli
authors: L
authors: L
authors: Paulesu
authors: F
authors: F
authors: Ietta
mesh_terms: D001280:Atrazine / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006063:Chorionic Gonadotropin / Q000378:metabolism; D004054:Diethylstilbestrol / Q000633:toxicity; D004785:Environmental Pollutants / Q000633:toxicity*; D004958:Estradiol / Q000633:toxicity*; D004967:Estrogens / Q000633:toxicity*; D006801:Humans; D010636:Phenols / Q000633:toxicity; D000077185:Resveratrol; D013267:Stilbenes / Q000633:toxicity; D014327:Trophoblasts / Q000187:drug effects* / Q000378:metabolism
chemical_list: D006063:Chorionic Gonadotropin; D004785:Environmental Pollutants; D004967:Estrogens; D010636:Phenols; D013267:Stilbenes; D004958:Estradiol; D004054:Diethylstilbestrol; C041594:4-nonylphenol; D000077185:Resveratrol; D001280:Atrazine