title: Histological evaluation of bone response to pediatric endodontic pastes: an experimental study in guinea pig.
abstract: This study aimed to evaluate by the intra-osseous implant technique the most commonly used materials for pulp therapy in pediatric dentistry: calcium hydroxide (CH), Guedes Pinto paste and CTZ paste, according to FDI (1980) and ANSI/ADA (1982) recommendations. Thirty guinea pigs, 10 for each material, divided into experimental periods of 4 and 12 weeks received one implant on each side of the lower jaw symphysis. The external lateral tube wall served as control for the technique. At the end of the observation periods, the animals were euthanized and specimens were prepared for routine histological examination. It was observed that CH and CTZ paste induced severe inflammation, a large amount of necrotic tissue, lymphocytes, foreign body cells and bone resorption, while Guedes Pinto Paste induced little or no inflammation in the 4-week observation period. After 12 weeks, the reactions to CH and Guedes Pinto paste were also absent/mild, presenting a general pattern of replacement by recently formed bone tissue while a moderate to severe inflammatory response was observed with CTZ paste. Guedes Pinto paste presented acceptable biocompatibility levels in both analyzed periods; CH only showed acceptable biocompatibility in the 12-week period while CTZ paste showed no biocompatibility in both periods. Among the tested materials, only Guedes Pinto paste presented an acceptable biocompatibility.
authors: Department of Endodontics, Dental School, UFU - Federal University of Uberlândia, Uberlândia, MG, Brazil.
authors: Andréa Mara
authors: AM
authors: Lacativa
authors: Adriano M
authors: AM
authors: Loyola
authors: Cassio José Alves
authors: CJ
authors: Sousa
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001672:Biocompatible Materials / Q000494:pharmacology*; D001862:Bone Resorption / Q000139:chemically induced; D002126:Calcium Hydroxide / Q000494:pharmacology; D002701:Chloramphenicol / Q000494:pharmacology; D004338:Drug Combinations; D005054:Eugenol / Q000494:pharmacology; D015743:Giant Cells, Foreign-Body / Q000187:drug effects; D006168:Guinea Pigs; D006847:Hydrocarbons, Iodinated / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects; D008334:Mandible / Q000187:drug effects*; D009336:Necrosis; D009504:Neutrophils / Q000187:drug effects; D010000:Osteitis / Q000139:chemically induced; D010012:Osteogenesis / Q000187:drug effects; D011239:Prednisolone / Q000031:analogs & derivatives / Q000494:pharmacology; D012294:Rifamycins / Q000494:pharmacology; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D013752:Tetracycline / Q000494:pharmacology; D013997:Time Factors; D015034:Zinc Oxide / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D001672:Biocompatible Materials; D004338:Drug Combinations; D006847:Hydrocarbons, Iodinated; D012294:Rifamycins; D012387:Root Canal Filling Materials; C541391:rifocort; D005054:Eugenol; D002701:Chloramphenicol; D011239:Prednisolone; D013752:Tetracycline; C010473:iodoform; D002126:Calcium Hydroxide; D015034:Zinc Oxide