title: Pro-oxidative, genotoxic and cytotoxic properties of uranyl ions.
abstract: It is demonstrated that hydroxyl radicals and hydrogen peroxide are formed under the action of uranyl ions in aqueous solutions containing no reducing agents. In the presence of uranyl ions, formation of 8-oxoguanine in DNA and long-lived protein radicals are observed in vitro. It is shown that the pro-oxidant properties of uranyl at micromolar concentrations mostly result from the physico-chemical nature of the compound rather than its radioactive decay. Uranyl ions lead to damage in DNA and proteins causing death of HEp-2 cells by necrotic pathway. It is revealed that the uranyl ions enhance radiation-induced oxidative stress and significantly increase a death rate of mice exposed to sublethal doses of X-rays.
authors: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia; Pushchino State University, Pushchino, Moscow Region 142290, Russia.
authors: S A
authors: SA
authors: Garmash
authors: V S
authors: VS
authors: Smirnova
authors: O E
authors: OE
authors: Karp
authors: A M
authors: AM
authors: Usacheva
authors: A V
authors: AV
authors: Berezhnov
authors: V E
authors: VE
authors: Ivanov
authors: A V
authors: AV
authors: Chernikov
authors: V I
authors: VI
authors: Bruskov
authors: S V
authors: SV
authors: Gudkov
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000737:chemistry; D001853:Bone Marrow / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage / Q000187:drug effects; D006147:Guanine / Q000031:analogs & derivatives; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000737:chemistry; D017665:Hydroxyl Radical / Q000032:analysis; D007477:Ions; D007834:Lasers; D008297:Male; D051379:Mice; D009026:Mortality; D009152:Mutagenicity Tests; D017382:Reactive Oxygen Species / Q000737:chemistry*; D014501:Uranium / Q000737:chemistry* / Q000633:toxicity*; D014502:Uranyl Nitrate / Q000737:chemistry; D014965:X-Rays
chemical_list: D001798:Blood Proteins; D007477:Ions; D017382:Reactive Oxygen Species; D014502:Uranyl Nitrate; D017665:Hydroxyl Radical; D014501:Uranium; C024829:8-hydroxyguanine; D006147:Guanine; D006861:Hydrogen Peroxide
keywords: 8-oxoGua; 8-oxoguanine; 8-oxoguanine (8-oxo-7,8-dihydroguanine); DMF; DU; Long-lived protein radicals; MN; PCE; ROS; Reactive oxygen species; Uranyl ions; depleted uranium; dose modification factor; micronuclei; polychromatophilic erythrocytes; reactive oxygen species