title: Curcumin protects against iron induced neurotoxicity in primary cortical neurons by attenuating necroptosis.
abstract: Necroptosis was reported as one backup way of programmed cell death when apoptosis was blocked, and the receptor interacting protein 1 was considered as the key necroptosis regulator protein. Here, we report the neuroprotective effects of curcumin which attenuates necroptosis. Primary cortical neurons were cultured and were injured by ferrous chloride, z.vad.fmk was applied to block apoptosis, curcumin was administrated to protect neurons, necrostatin-1 was applied to inhibit necroptosis if needed. Cell viability was measured by detecting lactate dehydrogenase activity in lysates of surviving cells, and assessed by cell counting kit-8. The expression of receptor interacting protein 1 was detected by immunoblot and immunofluorescence. Results showed that necroptosis mainly occurred in the concentrations of ferrous chloride ranging from 100 to 200μM, curcumin attenuated necroptosis in a dose-dependent manner. Furthermore, curcumin decreased expression of receptor interacting protein 1 in a dose- and time-dependent manner. Taken together, these findings suggest that curcumin protects against iron induced neurotoxicity in primary cortical neurons by attenuating necroptosis.
authors: Department of Neurosurgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025 China.
authors: Min-Chao
authors: MC
authors: Dai
authors: Zhi-Hong
authors: ZH
authors: Zhong
authors: Yu-Hao
authors: YH
authors: Sun
authors: Qing-Fang
authors: QF
authors: Sun
authors: Yong-Ting
authors: YT
authors: Wang
authors: Guo-Yuan
authors: GY
authors: Yang
authors: Liu-Guan
authors: LG
authors: Bian
mesh_terms: D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects* / Q000473:pathology; D003474:Curcumin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian / Q000166:cytology; D005296:Ferrous Compounds / Q000633:toxicity; D019190:Iron Overload / Q000473:pathology* / Q000517:prevention & control; D051379:Mice; D008810:Mice, Inbred C57BL; D009336:Necrosis; D009474:Neurons / Q000187:drug effects* / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*
chemical_list: D005296:Ferrous Compounds; D018696:Neuroprotective Agents; D003474:Curcumin; C029451:ferrous chloride