title: A simple blood-culture bacterial pellet preparation for faster accurate direct bacterial identification and antibiotic susceptibility testing with the VITEK 2 system.
abstract: An ammonium chloride procedure was used to prepare a bacterial pellet from positive blood cultures, which was used for direct inoculation of VITEK 2 cards. Correct identification reached 99% for Enterobacteriaceae and 74% for staphylococci. For antibiotic susceptibility testing, very major and major errors were 0.1 and 0.3% for Enterobacteriaceae, and 0.7 and 0.1% for staphylococci, respectively. Thus, bacterial pellets prepared with ammonium chloride allow direct inoculation of VITEK cards with excellent accuracy for Enterobacteriaceae and a lower accuracy for staphylococci.
authors: Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland.
authors: Guy
authors: G
authors: Prod'hom
authors: Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland.
authors: Christian
authors: C
authors: Durussel
authors: Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland.
authors: Gilbert
authors: G
authors: Greub
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001431:Bacteriological Techniques; D024881:Drug Resistance, Bacterial; D006090:Gram-Negative Bacteria / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification*; D006094:Gram-Positive Bacteria / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification*; D006801:Humans; D008826:Microbial Sensitivity Tests; D012680:Sensitivity and Specificity
chemical_list: D000900:Anti-Bacterial Agents