title: Optimization of LDL targeted nanostructured lipid carriers of 5-FU by a full factorial design.
abstract: BACKGROUND
  Nanostructured lipid carriers (NLC) are a mixture of solid and liquid lipids or oils as colloidal carrier systems that lead to an imperfect matrix structure with high ability for loading water soluble drugs. The aim of this study was to find the best proportion of liquid and solid lipids of different types for optimization of the production of LDL targeted NLCs used in carrying 5-Fu by the emulsification-solvent evaporation method.
  
  
  METHODS
  The influence of the lipid type, cholesterol or cholesteryl stearate for targeting LDL receptors, oil type (oleic acid or octanol), lipid and oil% on particle size, surface charge, drug loading efficiency, and drug released percent from the NLCs were studied by a full factorial design.
  
  
  RESULTS
  The NLCs prepared by 54.5% cholesterol and 25% of oleic acid, showed optimum results with particle size of 105.8 nm, relatively high zeta potential of -25 mV, drug loading efficiency of 38% and release efficiency of about 40%. Scanning electron microscopy of nanoparticles confirmed the results of dynamic light scattering method used in measuring the particle size of NLCs.
  
  
  CONCLUSIONS
  The optimization method by a full factorial statistical design is a useful optimization method for production of nanostructured lipid carriers.
authors: Department of Pharmaceutics, Faculty of Pharmacy and Novel Drug Delivery Systems Research Center, Isfahan University of Medical Sciences, Isfahan, India.
authors: Sare
authors: S
authors: Andalib
authors: Jaleh
authors: J
authors: Varshosaz
authors: Farshid
authors: F
authors: Hassanzadeh
authors: Hojjat
authors: H
authors: Sadeghi
keywords: 5-FU; LDL targeted nanostructured lipid carriers; optimization; structural parameters