title: X-ray microdiffraction and urine: a new analysis method of crystalluria.
abstract: The qualitative and quantitative analyses of crystalluria have clinical significance in the diagnosis and prognosis of urolithiasis. The aim of this paper is to provide a new accurate methodology to get qualitative and quantitative data on urine particulate in patients with renal stone disease.The procedure involves a urine collection, the separation of the solid residual by centrifugation, and its analysis by X-ray diffraction, utilizing a micro-diffractometer in order to analyze very low amounts of residual. The spectrum obtained was converted into 2 θ -I profiles and quantitatively refined by Rietveld method. The proposed methodology has the advantage to accurately quantify all crystalline phases and the amorphous component of the urine; anyway urine samples have to be centrifuged and analysed as soon as possible, because the quantitative results obtained by the X-ray microdiffraction showed that after some days and at room temperature urine increased significantly both amorphous and crystalline phases.
authors: Dip. Scienze Mediche e Chirurgiche, Università degli Studi di Padova, Padova, Italy.
authors: B
authors: B
authors: Baggio
authors: M L
authors: ML
authors: Giannossi
authors: L
authors: L
authors: Medici
authors: V
authors: V
authors: Summa
authors: F
authors: F
authors: Tateo
mesh_terms: D000328:Adult; D000368:Aged; D002129:Calcium Oxalate / Q000737:chemistry / Q000652:urine*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D017616:Magnesium Compounds / Q000737:chemistry / Q000652:urine*; D008297:Male; D008875:Middle Aged; D010710:Phosphates / Q000737:chemistry / Q000652:urine*; D015203:Reproducibility of Results; D000069877:Struvite; D013696:Temperature; D013997:Time Factors; D014527:Uric Acid / Q000737:chemistry / Q000652:urine*; D052878:Urolithiasis / Q000652:urine; D014961:X-Ray Diffraction / Q000379:methods*
chemical_list: D017616:Magnesium Compounds; D010710:Phosphates; D002129:Calcium Oxalate; D014527:Uric Acid; C016188:weddellite; D000069877:Struvite