title: Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green.
abstract: cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces H(2)O(2) over 7.0 nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of H(2)O(2) improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to 150 µM within 90 min.
authors: Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, Korea.
authors: Yu-Lim
authors: YL
authors: Son
authors: Hyoun-Young
authors: HY
authors: Kim
authors: Saravanakumar
authors: S
authors: Thiyagarajan
authors: Jing Jing
authors: JJ
authors: Xu
authors: Seung-Moon
authors: SM
authors: Park
keywords: Dye decolorization; Glyoxal oxidase; Manganese peroxidase; Phanerochaete chrysosporium; Pichia pastoris