title: Pretreatment effect of running exercise on HSP70 and DOX-induced cardiotoxicity.
abstract: OBJECTIVE
  The purpose of this study was to determine pretreatment effects of moderate-term endurance training before the various dosages (10 and 20 mg.kg(-1)) of DOX on a heat shock protein (HSP70 kda) and cardiotoxicity in heart tissue.
  
  
  METHODS
  Forty-eight male rats were randomly assigned to nontraining (NT) and training (T) groups and three subgroups; DOX10 mg.kg(-1) and DOX20 mg.kg(-1) and saline treatment. The training program included treadmill running between 25-39 min/day and 15-17 m/min, 5 days/wk for 3 wk.
  
  
  RESULTS
  DOX administration, in particularly with 20 mg.kg(-1), caused up-regulation of oxidants and cardiac damage (MDA, CK, CPK-MB and CK/ CPK-MB) and down-regulation of cardioprotection (HSP70, SOD) markers, as compared to NT+saline group. Pretreatment effect of treadmill running endurance exercise in the presence of DOX with 10 mg.kg(-1) caused a significant increase in HSP70, SOD and a significant decrease in MDA and insignificant decrease in CK, CPK-MB and CK/CPK-MB, in comparisonT+DOX10 with NT+DOX10 group. However, there was no significant difference between T+DOX10 mg.kg(-1) and T+DOX20 mg.kg(-1) in the aforesaid markers.
  
  
  CONCLUSIONS
  Dox-induced cardiotoxicity is related to oxidative stress. Our study suggests that pretreatment with endurance exercise may be considered as a potentially useful strategy to improve myocardial tolerance against single dose DOX-induced oxidative damage.
authors: College of Physical Education and Sport Sciences, Department of Sport Physiology, University of Mazandaran, Babolsar, Iran.
authors: Vahid
authors: V
authors: Shirinbayan
authors: Valiollah Dabidi
authors: VD
authors: Roshan
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D015415:Biomarkers / Q000032:analysis*; D004317:Doxorubicin / Q000494:pharmacology*; D005081:Exercise Therapy; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006331:Heart Diseases / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D018384:Oxidative Stress; D010805:Physical Conditioning, Animal; D051381:Rats; D017208:Rats, Wistar; D012420:Running
chemical_list: D000903:Antibiotics, Antineoplastic; D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; D004317:Doxorubicin