title: The cancer stem cell marker CD133 interacts with plakoglobin and controls desmoglein-2 protein levels.
abstract: The pentaspan membrane glycoprotein CD133 (also known as prominin-1) has been widely used as a marker for both cancer and normal stem cells. However, the function of CD133 has not been elucidated. Here we describe a cancer stem cell line established from clear cell carcinoma of the ovary (CCC) and show that CD133 interacts with plakoglobin (also known as γ-catenin), a desmosomal linker protein. We further demonstrate that knockdown of CD133 by RNA interference (RNAi) results in the downregulation of desmoglein-2, a desmosomal cadherin, and abrogates cell-cell adhesion and tumorigenicity of CCC stem cells. We speculate that CD133 may be a promising target for cancer chemotherapy.
authors: Laboratory of Molecular and Genetic Information, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors: Ryo
authors: R
authors: Koyama-Nasu
authors: Rina
authors: R
authors: Takahashi
authors: Satoshi
authors: S
authors: Yanagida
authors: Yukiko
authors: Y
authors: Nasu-Nishimura
authors: Masaaki
authors: M
authors: Oyama
authors: Hiroko
authors: H
authors: Kozuka-Hata
authors: Ryo
authors: R
authors: Haruta
authors: Emi
authors: E
authors: Manabe
authors: Akemi
authors: A
authors: Hoshino-Okubo
authors: Hiroko
authors: H
authors: Omi
authors: Nozomu
authors: N
authors: Yanaihara
authors: Aikou
authors: A
authors: Okamoto
authors: Tadao
authors: T
authors: Tanaka
authors: Tetsu
authors: T
authors: Akiyama
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D018938:Caco-2 Cells; D002448:Cell Adhesion; D002471:Cell Transformation, Neoplastic / Q000378:metabolism / Q000473:pathology; D051301:Desmoglein 2 / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D051379:Mice; D014411:Neoplastic Stem Cells / Q000378:metabolism* / Q000473:pathology; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding; D051185:gamma Catenin / Q000378:metabolism*
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D014408:Biomarkers, Tumor; C495314:DSG2 protein, human; D051301:Desmoglein 2; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse; D051185:gamma Catenin