title: NEDD8 conjugation in Schistosoma mansoni: genome analysis and expression profiles.
abstract: NEDD8 is an ubiquitin-like molecule that covalently binds to target proteins through an enzymatic cascade analogous to ubiquitylation. This modifier is known to bind to p53 and p73, as well as all Cullin family proteins, which are essential components of Skp1/Cul-1/F-box protein (SCF)-like Ub ligase complexes. Here, we focused on a genomic analysis of the genes involved in the NEDD8 conjugation pathway in Schistosoma mansoni. The results revealed seven genes related to NEDD8 conjugation that are conserved in Schistosoma japonicum, Caenorhabditis elegans, Drosophila melanogaster and Homo sapiens. We performed quantitative RT-PCR (qRT-PCR), which showed differential profiles for Smnedd8, Smapp1, Smuba3, Smube2f, Smdcn1, Smrbx and Smsenp8 throughout the life cycle of S. mansoni. Upregulation was observed in 3-day-old schistosomula and adult worms for all analysed genes. We also analysed the transcription levels of Cullin family members Smp63 and Smp73, and observed upregulation in early schistosomula, while cercariae and adult worms showed expression levels similar to one another. Taken together, these results suggest that the NEDDylation/DeNEDDylation pathway controls important cellular regulators during worm development from cercariae to schistosomula and, finally, to adult.
authors: Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Morro do Cruzeiro, Ouro Preto, MG, Brazil.
authors: Roberta V
authors: RV
authors: Pereira
authors: Matheus de S
authors: Mde S
authors: Gomes
authors: Roenick P
authors: RP
authors: Olmo
authors: Daniel M
authors: DM
authors: Souza
authors: Liana K
authors: LK
authors: Jannotti-Passos
authors: Elio H
authors: EH
authors: Baba
authors: William
authors: W
authors: Castro-Borges
authors: Renata
authors: R
authors: Guerra-Sá
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001700:Biomphalaria; D044842:Cullin Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D023281:Genomics; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010802:Phylogeny; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D018510:RNA, Helminth / Q000235:genetics; D012550:Schistosoma mansoni / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D012555:Schistosomiasis mansoni / Q000469:parasitology; D016415:Sequence Alignment; D059467:Transcriptome; D044763:Ubiquitin-Conjugating Enzymes / Q000235:genetics* / Q000378:metabolism; D043743:Ubiquitin-Protein Ligase Complexes / Q000235:genetics / Q000378:metabolism; D014452:Ubiquitins / Q000235:genetics* / Q000378:metabolism
chemical_list: D044842:Cullin Proteins; D015801:Helminth Proteins; D018510:RNA, Helminth; D014452:Ubiquitins; D044763:Ubiquitin-Conjugating Enzymes; D043743:Ubiquitin-Protein Ligase Complexes