title: Proteomic changes induced by podophyllotoxin in human cervical carcinoma HeLa cells.
abstract: Podophyllotoxin, a kind of lignan extracted from the Podophyllum plant, has been shown to inhibit the growth of various carcinoma cells. However, the molecular mechanism remains unclear. In this study, the inhibition of cell growth and changes in protein expression induced by podophyllotoxin were investigated in human cervical carcinoma  HeLa  cells. Our results demonstrate that Podophyllotoxin inhibits  HeLa  cell growth and induces apoptosis. By using proteomic techniques, seven proteins were found to be significantly regulated by podophyllotoxin compared to the untreated control; among them, four were down-regulated and three were up-regulated. All of the seven proteins were identified with peptide mass fingerprinting using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) after in-gel trypsin digestion. Five of these proteins are involved in protein metabolism, and the other two play roles in cell communication and signaling transduction pathways. It is suggested that the effect of podophyllotoxin on the growth of tumor cells is significantly related to the metabolism-associated proteins.
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237,  P. R. China.
authors: Bochan
authors: B
authors: Wang
authors: Lifeng
authors: L
authors: Chen
authors: Hong
authors: H
authors: Zhen
authors: Li
authors: L
authors: Zhou
authors: Ping
authors: P
authors: Shi
authors: Zhiwei
authors: Z
authors: Huang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D002450:Cell Communication; D005260:Female; D006367:HeLa Cells; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism* / Q000502:physiology; D011034:Podophyllotoxin / Q000494:pharmacology*; D040901:Proteomics; D015398:Signal Transduction; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D009363:Neoplasm Proteins; D011034:Podophyllotoxin