title: Impact of polymer shell on the formation and time evolution of nanoparticle-protein corona.
abstract: The study of protein corona formation on nanoparticles (NPs) represents an actual main issue in colloidal, biomedical and toxicological sciences. However, little is known about the influence of polymer shells on the formation and time evolution of protein corona onto functionalized NPs. Therefore, silica-poly(ethylene glycol) core-shell nanohybrids (SNPs@PEG) with different polymer molecular weights (MW) were synthesized and exhaustively characterized. Bovine serum albumin (BSA) at different concentrations (0.1-6 wt%) was used as model protein to study protein corona formation and time evolution. For pristine SNPs and SNPs@PEG (MW=350 g/mol), zeta potential at different incubation times show a dynamical evolution of the nanoparticle-protein corona. Oppositely, for SNPs@PEG with MW≥2000 g/mol a significant suppression of corona formation and time evolution was observed. Furthermore, AFM investigations suggest a different orientation (side-chain or perpendicular) and penetration depth of BSA toward PEGylated surfaces depending on the polymer length which may explain differences in protein corona evolution.
authors: BAM Federal Institute for Materials Research and Testing, Unter den Eichen 87, 12205 Berlin, Germany.
authors: Kishore
authors: K
authors: Natte
authors: Jörg F
authors: JF
authors: Friedrich
authors: Sebastian
authors: S
authors: Wohlrab
authors: Jana
authors: J
authors: Lutzki
authors: Regine
authors: R
authors: von Klitzing
authors: Werner
authors: W
authors: Österle
authors: Guillermo
authors: G
authors: Orts-Gil
mesh_terms: D000818:Animals; D002417:Cattle; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors
chemical_list: D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D012822:Silicon Dioxide