title: Validation of a commercially available indirect ELISA using a nucleocapside recombinant protein for detection of Schmallenberg virus antibodies.
abstract: A newly developed Enzym Like Immuno Sorbant Assay (ELISA) based on the recombinant nucleocapsid protein (N) of Schmallenberg virus (SBV) was evaluated and validated for the detection of SBV-specific IgG antibodies in ruminant sera by three European Reference Laboratories. Validation data sets derived from sheep, goat and bovine sera collected in France and Germany (n = 1515) in 2011 and 2012 were categorized according to the results of a virus neutralization test (VNT) or an indirect immuno-fluorescence assay (IFA). The specificity was evaluated with 1364 sera from sheep, goat and bovine collected in France and Belgium before 2009. Overall agreement between VNT and ELISA was 98.9% and 98.3% between VNT and IFA, indicating a very good concordance between the different techniques. Although cross-reactions with other Orthobunyavirus from the Simbu serogroup viruses might occur, it is a highly sensitive, specific and robust ELISA-test validated to detect anti-SBV antibodies. This test can be applied for SBV sero-diagnostics and disease-surveillance studies in ruminant species in Europe.
authors: Virology Unit, French Agency for Food, Environmental and Occupational Health and Safety, Maisons-Alfort, France. emmanuel.breard@anses.fr
authors: Emmanuel
authors: E
authors: Bréard
authors: Estelle
authors: E
authors: Lara
authors: Loïc
authors: L
authors: Comtet
authors: Cyril
authors: C
authors: Viarouge
authors: Virginie
authors: V
authors: Doceul
authors: Alexandra
authors: A
authors: Desprat
authors: Damien
authors: D
authors: Vitour
authors: Nathalie
authors: N
authors: Pozzi
authors: Ann Brigitte
authors: AB
authors: Cay
authors: Nick
authors: N
authors: De Regge
authors: Philippe
authors: P
authors: Pourquier
authors: Horst
authors: H
authors: Schirrmeier
authors: Bernd
authors: B
authors: Hoffmann
authors: Martin
authors: M
authors: Beer
authors: Corinne
authors: C
authors: Sailleau
authors: Stéphan
authors: S
authors: Zientara
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D002044:Bunyaviridae Infections / Q000175:diagnosis* / Q000662:veterinary; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005060:Europe; D019084:Fluorescent Antibody Technique, Indirect; D015870:Gene Expression; D009500:Neutralization Tests; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000276:immunology*; D016851:Orthobunyavirus / Q000235:genetics / Q000276:immunology*; D012372:ROC Curve; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D015203:Reproducibility of Results; D012756:Sheep
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D019590:Nucleocapsid Proteins; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins