title: Bone regeneration with a combination of nanocrystalline hydroxyapatite silica gel, platelet-rich growth factor, and mesenchymal stem cells: a histologic study in rabbit calvaria.
abstract: OBJECTIVE
  This study aimed to assess NanoBone as a carrier construct for mesenchymal stem cells (MSCs) and platelet-rich growth factor (PRGF).
  
  
  METHODS
  In the calvarial bone of 8 mature New Zealand White male rabbits, four 8-mm defects were created. Each defect received one of the following treatments: Group 1, 0.2 mg Nano-hydroxyapatite (HA) granule + 2 mL culture medium; Group 2, 0.2 mg Nano-HA + 1 mL autologous PRGF + 2 mL acellular culture medium; Group 3, 0.2 mg Nano-HA + 2 mL culture medium containing 100,000 autogenous MSCs; Group 4, 0.2 mg Nano-HA + 2 mL culture medium containing 100,000 autogenous MSCs + 1 mL autologous PRGF.
  
  
  RESULTS
  Histomorphometric analysis at 6 and 12 weeks demonstrated significantly higher bone formation in group 4 (29.45% and 44.55%, respectively) (P < .05). Bone formation in groups 1, 2, and 3 were as follows: 11.35% and 32.53%, 29.10% and 39.74%, and 25.82% and 39.11%, respectively.
  
  
  CONCLUSIONS
  NanoBone with MSCs and PRGF seems to be an effective combination for bone regeneration in a rabbit calvaria model.
authors: Department of Oral and Maxillofacial Surgery, Dental Research Center, Shahid Beheshti Medical University, Tehran, Islamic Republic of Iran.
authors: Hossein
authors: H
authors: Behnia
authors: Arash
authors: A
authors: Khojasteh
authors: Mohammad Taghi
authors: MT
authors: Kiani
authors: Ahad
authors: A
authors: Khoshzaban
authors: Fatemeh
authors: F
authors: Mashhadi Abbas
authors: Maryam
authors: M
authors: Bashtar
authors: Seyedeh Ghazaleh
authors: SG
authors: Dashti
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D018786:Bone Substitutes / Q000494:pharmacology*; D004338:Drug Combinations; D017886:Durapatite / Q000494:pharmacology*; D005434:Flow Cytometry; D008297:Male; D059630:Mesenchymal Stem Cells; D008855:Microscopy, Electron, Scanning; D053657:Platelet-Rich Plasma; D011817:Rabbits; D012822:Silicon Dioxide / Q000494:pharmacology*; D012886:Skull / Q000601:surgery*; D018709:Statistics, Nonparametric
chemical_list: D018786:Bone Substitutes; D004338:Drug Combinations; C534915:NanoBone; D012822:Silicon Dioxide; D017886:Durapatite