title: Serial evaluation of neutrophil function in tumour-bearing dogs undergoing chemotherapy.
abstract: We hypothesized that neutrophil function in tumour-bearing dogs is negatively impacted by chemotherapy. Flow cytometric techniques were used to assess neutrophil oxidative burst and phagocytic activities at baseline, 7 and 21 days after induction chemotherapy in 20 dogs with lymphoma. Dogs had a lower percentage of neutrophils exhibiting oxidative burst activity after stimulation with Escherichia coli (day 7; P = 0.009) and phorbol 12-myristate 13-acetate (PMA) (days 7 and 21; P = 0.0003 and P = 0.01, respectively), compared with healthy controls. From day 0 to 7, the percentage of neutrophils exhibiting oxidative burst activity decreased after stimulation with E. coli (P = 0.016) and PMA (P = 0.0006). Induction chemotherapy suppresses the percentage of neutrophils capable of oxidative burst in dogs with lymphoma, with improvement in phagocytic activity over time (P = 0.03). The impact of neutrophil dysfunction on incidence and severity of sepsis in dogs receiving chemotherapy should be investigated.
authors: Department of Small Animal Clinical Sciences, The University of Tennessee, Knoxville, TN, USA.
authors: A K
authors: AK
authors: LeBlanc
authors: C J
authors: CJ
authors: LeBlanc
authors: B W
authors: BW
authors: Rohrbach
authors: S A
authors: SA
authors: Kania
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000188:drug therapy* / Q000276:immunology; D004285:Dogs; D009369:Neoplasms / Q000188:drug therapy / Q000276:immunology / Q000662:veterinary*; D009503:Neutropenia / Q000139:chemically induced / Q000662:veterinary*; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D016897:Respiratory Burst
keywords: cancer; canine; chemotherapy; flow cytometry; oxidative burst; phagocytosis