title: Use of Gene Ontology Annotation to understand the peroxisome proteome in humans.
abstract: The Gene Ontology (GO) is the de facto standard for the functional description of gene products, providing a consistent, information-rich terminology applicable across species and information repositories. The UniProt Consortium uses both manual and automatic GO annotation approaches to curate UniProt Knowledgebase (UniProtKB) entries. The selection of a protein set prioritized for manual annotation has implications for the characteristics of the information provided to users working in a specific field or interested in particular pathways or processes. In this article, we describe an organelle-focused, manual curation initiative targeting proteins from the human peroxisome. We discuss the steps taken to define the peroxisome proteome and the challenges encountered in defining the boundaries of this protein set. We illustrate with the use of examples how GO annotations now capture cell and tissue type information and the advantages that such an annotation approach provides to users. Database URL: http://www.ebi.ac.uk/GOA/ and http://www.uniprot.org.
authors: EMBL-EBI, The Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SD, UK. prudence@ebi.ac.uk
authors: Prudence
authors: P
authors: Mutowo-Meullenet
authors: Rachael P
authors: RP
authors: Huntley
authors: Emily C
authors: EC
authors: Dimmer
authors: Yasmin
authors: Y
authors: Alam-Faruque
authors: Tony
authors: T
authors: Sawford
authors: Maria
authors: M
authors: Jesus Martin
authors: Claire
authors: C
authors: O'Donovan
authors: Rolf
authors: R
authors: Apweiler
mesh_terms: D030562:Databases, Protein; D006801:Humans; D058977:Molecular Sequence Annotation; D009928:Organ Specificity; D020675:Peroxisomes / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D013045:Species Specificity
chemical_list: D020033:Protein Isoforms; D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins