title: Combinational biosynthesis of phycocyanobilin using genetically-engineered Escherichia coli.
abstract: Genes of the key enzymes for phycocyanobilin (PCB) biosynthesis were cloned into E. coli and combinationally expressed to produce phycocyanobilin, with autologous heme as substrate. Culture conditions were optimized to achieve ~3 mg PCB/l. A protocol for the purification of recombinant phycocyanobilin was established using solvent extraction combined with chromatography, which resulted in a final yield of ~0.3 mg PCB/l with a purity >95 %. Recombinant phycocyanobilin could scavenge hydroxyl radicals with an EC50 of 0.1 μM.
authors: Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao 266580, People's Republic of China.
authors: Baosheng
authors: B
authors: Ge
authors: Yan
authors: Y
authors: Li
authors: Haixiang
authors: H
authors: Sun
authors: Shuai
authors: S
authors: Zhang
authors: Peijie
authors: P
authors: Hu
authors: Song
authors: S
authors: Qin
authors: Fang
authors: F
authors: Huang
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D001713:Biphenyl Compounds / Q000032:analysis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D052980:Phycobilins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D010798:Phycocyanin / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D010851:Picrates / Q000032:analysis / Q000378:metabolism; D013050:Spectrometry, Fluorescence
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D052980:Phycobilins; D010851:Picrates; D010798:Phycocyanin; C011069:phycocyanobilin; C004931:1,1-diphenyl-2-picrylhydrazyl