title: Induction of neuritogenesis in PC12 cells by a pulsed electromagnetic field via MEK-ERK1/2 signaling.
abstract: We examined the regulation of neuritogenesis by a pulsed electromagnetic field (PEMF) in rat PC12 pheochromocytoma cells, which can be induced to differentiate into neuron-like cells with elongated neurites by inducers such as nerve growth factor (NGF). Plated PC12 cells were exposed to a single PEMF (central magnetic flux density, 700 mT; frequency, 0.172 Hz) for up to 12 h per day and were then evaluated for extent of neuritogenesis or acetylcholine esterase (AChE) activity. To analyze the mechanism underlying the effect of the PEMF on the cells, its effects on intracellular signaling were examined using the ERK kinase (MEK) inhibitors PD098059 and U0126 (U0124 was used as a negative control for U0126). The number of neurite-bearing PC12 cells and AChE activity increased after PEMF exposure without the addition of other inducers of neuritogenesis. Additionally, PEMF exposure induced sustained activation of ERK1/2 in PC12 cells, but not in NR8383 rat alveolar macrophages. Furthermore, U0126 strongly inhibited PEMF-dependent ERK1/2 activation and neuritogenesis. The PEMF-dependent neuritogenesis was also suppressed by PD098059, but not U0124. These results suggest that PEMF stimulation independently induced neuritogenesis and that activation of MEK-ERK1/2 signaling was induced by a cell-type-dependent mechanism required for PEMF-dependent neuritogenesis in PC12 cells.
authors: Division of Oral Physiology, Tohoku University Graduate School of Dentistry, Sendai, Japan. tkudo@m.tohoku.ac.jp
authors: Tada-aki
authors: TA
authors: Kudo
authors: Hiroyasu
authors: H
authors: Kanetaka
authors: Yoshinaka
authors: Y
authors: Shimizu
authors: Toshihiko
authors: T
authors: Abe
authors: Hitoshi
authors: H
authors: Mori
authors: Kazumi
authors: K
authors: Mori
authors: Eizaburo
authors: E
authors: Suzuki
authors: Toshiyuki
authors: T
authors: Takagi
authors: Shin-ichi
authors: S
authors: Izumi
mesh_terms: D000818:Animals; D002070:Butadienes / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D004574:Electromagnetic Fields; D005419:Flavonoids / Q000494:pharmacology; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000528:radiation effects; D016676:Macrophages, Alveolar / Q000378:metabolism / Q000528:radiation effects; D020932:Nerve Growth Factor / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D016501:Neurites / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D009570:Nitriles / Q000494:pharmacology; D016716:PC12 Cells; D051381:Rats
chemical_list: D002070:Butadienes; D005419:Flavonoids; D009570:Nitriles; C113580:U 0126; D020932:Nerve Growth Factor; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one