title: Cloning and polymorphism analysis of the 2-methyl-6-phytyl-1,4-benzoquinol methyltransferase gene (VTE3) in Arachis hypogaea, A. duranensis, and A. ipaënsis.
abstract: One of the critical enzymes involved in vitamin E biosynthesis in plants is 2-methyl-6-phytyl-1,4-benzoquinol methyltransferase (MPBQ MT). The full-length VTE3 cDNA (designated rVTE3-1 and -2) encoding MPBQ MT and the full-length DNA of VTE3 (designated gVTE3-1 and -2) were isolated from cultivated peanuts (Arachis hypogaea). The full-length DNA of VTE3 (designated gVTE3-A and -B) was isolated from the wild groundnut species A. duranensis (A-genome) and A. ipaënsis (B-genome), and polymorphism analysis of VTE3 was performed. The results demonstrated that rVTE3-1 and -2 both have a DNA sequence that is 1059 bp long and encodes 351 amino acids; the homology of the 2 amino acid sequences was 98.6%. The gVTE3-1 of cultivated Fenghua 2 peanut samples was 2710 bp long, with 3 introns located at 44-163, 772-1295, and 1603-2437 bp, and the Fenghua 2 gVTE3-2 was 2706 bp long, with 3 introns located at 44-169, 778-1291, and 1599-2433 bp. The homology for gVTE3-1 and -2 across 13 cultivated peanut samples was 99.9 and 100%, respectively. gVTE3-1 and -2 were from A- and B-genome, respectively, with 96.6% homology between the 2 sequences. The present study demonstrated that abundant polymorphisms were present in the VTE3 genes from different genomes. Additionally, polymorphisms were observed in the gVTE3-1 alleles of the 13 cultivars and wild A. duranensis species but not in the gVTE3-2 alleles of the 13 cultivars and wild A. ipaënsis species.
authors: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian, Shandong, China. liufz@sdau.edu.cn
authors: F Z
authors: FZ
authors: Liu
authors: A Q
authors: AQ
authors: Guo
authors: Y S
authors: YS
authors: Wan
mesh_terms: D000595:Amino Acid Sequence; D010367:Arachis / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D003001:Cloning, Molecular; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics; D007887:Fabaceae / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D017343:Genes, Plant / Q000235:genetics*; D008780:Methyltransferases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000235:genetics; D010802:Phylogeny; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D018076:DNA, Complementary; D009711:Nucleotides; D008780:Methyltransferases