title: Development of a biomimetic enzyme-linked immunosorbent assay method based on a hydrophilic molecularly imprinted polymer film for determination of olaquindox in chick feed samples.
abstract: In this study, a new way to substitute the biology antibody was introduced by using a hydrophilic molecularly imprinted film, which was directly prepared on the well surface of MaxiSorp polystyrene 96-well plate by the bulk polymerization technique. This imprinted film exhibited good recognition and fast adsorption-desorption dynamics toward olaquindox. Using it as the recognition element, a fast and new direct competitive biomimetic enzyme-linked immunosorbent assay (BELISA) method for the determination of olaquindox in chick feed was developed. This BELISA method had low cross-reactivities of 6.2% and 12% for two analogues. Under the optimal conditions, the sensitivity (IC50) and the limit of detection (IC15) were 700 ± 60 µg L(-1) and 17.0 ± 1.6 µg L(-1), respectively. The blank chick feed samples spiked with olaquindox at three levels were determined by this developed method with recoveries ranging from 82.0-96.0%.
authors: College of Food Science and Engineering, Shandong Agricultural University, Taian, China.
authors: Dongyan
authors: D
authors: Zhao
authors: Xuguang
authors: X
authors: Qiao
authors: Zhixiang
authors: Z
authors: Xu
authors: Rui
authors: R
authors: Xu
authors: Zhenhua
authors: Z
authors: Yan
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis* / Q000276:immunology; D000906:Antibodies / Q000032:analysis; D032701:Biomimetics / Q000379:methods; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005506:Food Contamination / Q000032:analysis; D006133:Growth Substances / Q000032:analysis* / Q000276:immunology; D057927:Hydrophobic and Hydrophilic Interactions; D054802:Molecular Imprinting; D011137:Polystyrenes; D011810:Quinoxalines / Q000032:analysis* / Q000276:immunology
chemical_list: D000890:Anti-Infective Agents; D000906:Antibodies; D006133:Growth Substances; D011137:Polystyrenes; D011810:Quinoxalines; C013168:olaquindox