title: Model for generation of large numbers of primary, inflammatory skin-derived neutrophils, and macrophages.
abstract: Tissue macrophages and inflammatory neutrophils represent important cells of the innate immune system responsible for various important tasks, i.e., elimination of pathogens and/or granuloma formation. Isolation of large numbers of primary phagocytes is vital for research with these cells. Within this protocol, we present a strategy for isolation of large numbers of inflammatory neutrophils and macrophages from murine skin that allows for follow-up in vivo or in vitro studies.
authors: Department of Dermatology, University Medical Center, Johannes Gutenberg-University, Mainz, Germany.
authors: Dominik
authors: D
authors: John
authors: Michael R
authors: MR
authors: Fischer
authors: Esther
authors: E
authors: von Stebut
mesh_terms: D000180:Acrylic Resins; D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D006099:Granuloma / Q000139:chemically induced* / Q000276:immunology / Q000473:pathology; D007113:Immunity, Innate; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology / Q000473:pathology; D008264:Macrophages / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000276:immunology* / Q000473:pathology; D012867:Skin / Q000166:cytology* / Q000276:immunology / Q000473:pathology; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D000099166:Polyacrylamides