title: Differential effects of the persistent DDT metabolite methylsulfonyl-DDE in nonstimulated and LH-stimulated neonatal porcine Leydig cells.
abstract: 3-Methylsulfonyl-DDE (MeSO₂-DDE) is a potent adrenal toxicant formed from the persistent insecticide DDT. MeSO₂-DDE is widely present in human plasma, milk and fat, and in tissues of marine mammals. In the present study, we investigated endocrine-disrupting properties of MeSO₂-DDE in primary neonatal porcine Leydig cells. Unstimulated and LH-stimulated cells were exposed to MeSO₂-DDE at concentrations ranging from 0.6 to 20 μM for 48 h. Cell viability, hormone secretion and expression of steroidogenesis related genes were recorded. Secretion of testosterone and estradiol was increased in a concentration-dependent fashion in unstimulated Leydig cells, while in LH-stimulated cells, secretion of testosterone, estradiol and progesterone was decreased. The expression of important steroidogenic genes was down-regulated both in unstimulated and LH-stimulated cells. Notably, no significant impairment of cell viability occurred at any exposure except the highest concentration (20 μM) in LH-stimulated cells. This indicated that the effects on hormone secretion and gene expression were not caused by cytotoxicity. We conclude that the adrenal toxicant MeSO₂-DDE disrupts hormone secretion in a complex fashion in neonatal porcine Leydig cells. The different endocrine responses in unstimulated and LH-stimulated cells imply that the endocrine disruptive activity of MeSO₂-DDE is determined by the physiological status of the Leydig cells.
authors: Department of Production Animal Clinical Sciences, Norwegian School of Veterinary Science, Postboks 8146 Dep, 0033 Oslo, Norway. cesilie.castellanos@nvh.no
authors: Cesilie Granum
authors: CG
authors: Castellanos
authors: Irene Beate
authors: IB
authors: Sørvik
authors: Marte Bruu
authors: MB
authors: Tanum
authors: Steven
authors: S
authors: Verhaegen
authors: Ingvar
authors: I
authors: Brandt
authors: Erik
authors: E
authors: Ropstad
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003634:DDT / Q000378:metabolism / Q000633:toxicity*; D003633:Dichlorodiphenyl Dichloroethylene / Q000378:metabolism / Q000633:toxicity*; D052244:Endocrine Disruptors / Q000633:toxicity*; D007985:Leydig Cells / Q000187:drug effects* / Q000378:metabolism; D007986:Luteinizing Hormone / Q000633:toxicity*; D008297:Male; D013552:Swine
chemical_list: D052244:Endocrine Disruptors; D003633:Dichlorodiphenyl Dichloroethylene; D007986:Luteinizing Hormone; D003634:DDT