title: Impact of ketorolac administration around ovarian stimulation on in vivo and in vitro fertilization and subsequent embryo development.
abstract: We performed this study to investigate the effect of ketorolac (a non-steroidal anti-inflammatory drug) administration around ovarian stimulation on in vivo and in vitro fertilization process. Sixty-four female mice (ICR) were injected with ketorolac (0, 7.5, 15 and 30 µg/d) for 3 d starting from the day of eCG treatment. In experiment 1, 41 mice were triggered by hCG and then mated; two-cell embryos were obtained and in vitro development up to blastocyst was observed. In experiment 2, 23 mice were triggered by hCG and mature oocytes were collected; in vitro fertilization rate and subsequent embryo development up to blastocyst was recorded. In experiment 1, the blastocyst-forming rates per in vivo fertilized two-cell embryo showed an inverse relationship with a dosage of ketorolac (97.6%, 64.2%, 35.4% and 25.9%). In experiment 2, degenerated oocytes were frequently observed in a dose-dependent manner (4.3%, 22.9%, 22.4% and 75.0%). Lower fertilization rates were noted in all the three ketorolac-treating groups; blastocyst-forming rate was significantly lower in 30-µg-treating group when compared with the control group. Administration of ketorolac around ovarian stimulation significantly affects the development of in vivo fertilized embryo in a dose-dependent manner. High-dose ketorolac could result in a poor oocyte quality and decreased embryo developmental competence.
authors: Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul , Korea.
authors: Byung Chul
authors: BC
authors: Jee
authors: Hye Won
authors: HW
authors: Youm
authors: Jae Ho
authors: JH
authors: Lee
authors: Jee Hyun
authors: JH
authors: Kim
authors: Chang Suk
authors: CS
authors: Suh
authors: Seok Hyun
authors: SH
authors: Kim
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000009:adverse effects*; D004622:Embryo, Mammalian / Q000187:drug effects; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D005306:Fertilization / Q000187:drug effects*; D005307:Fertilization in Vitro / Q000187:drug effects*; D020910:Ketorolac / Q000008:administration & dosage / Q000009:adverse effects*; D051379:Mice; D008813:Mice, Inbred ICR; D010062:Ovulation Induction
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D020910:Ketorolac