title: [Usefulness of hepatitis C virus (HCV) genotype test using direct sequencing of 5' untranslated region--application from 2003 to 2011].
abstract: Hepatitis C virus (HCV) genotype is the key to prediction of the therapeutic effect of combination therapy of pegylated interferon and ribavirin. HCV grouping test, which is used as a routine testing method, is simple and practical, but the results are sometimes not conclusive. In order to cope with such cases, we have adopted a direct sequencing method since 2003. This paper summarizes the HCV genotype test results obtained between 2003 and 2011. The results showed that the genotypes of 129 of 255 specimens which were not determined by the HCV grouping test were all successfully determined by the direct sequencing method. In the serotype determined samples the concordance rate with direct sequencing was 99.0% in HCV serotype 1 and 88.0% in HCV serotype 2. These findings suggest that the direct sequencing method is useful to determine the genotypes of specimens when untypeable with the HCV grouping test.
authors: Department of Clinical Laboratory, The University of Tokyo Hospital, Bunkyo-ku, Tokyo 113-8655, Japan.
authors: Rie
authors: R
authors: Maruo
authors: Yumiko
authors: Y
authors: Sato
authors: Hiromitsu
authors: H
authors: Yokota
authors: Yuko
authors: Y
authors: Kageyama
authors: Kenichi
authors: K
authors: Syukuya
authors: Yutaka
authors: Y
authors: Yatomi
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D005838:Genotype; D016174:Hepacivirus / Q000235:genetics* / Q000302:isolation & purification; D006526:Hepatitis C / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*; D012703:Serotyping / Q000379:methods; D013997:Time Factors
chemical_list: D020121:5' Untranslated Regions