title: Therapeutic outcomes of transplanting autologous granulocyte colony-stimulating factor-mobilised peripheral mononuclear cells in diabetic patients with critical limb ischaemia.
abstract: The efficacy and safety of transplanting autologous mesenchymal stem cells (MSCs), from granulocyte-colony-stimulating factor (G-CSF)-mobilised peripheral blood, was investigated in diabetic patients with critical limb ischaemia (CLI). After 3 months, the transplanted group of patients (n=7) showed a significant improvement in ischaemia manifestations, including pain and neurological signs, wound healing and the rate of lower-limb amputation, compared to the control group of patients (n=14). Pain was significantly reduced in the transplanted group compared to controls (P=0.014). The ankle-brachial index (ABI) and the pulse strength within ischaemic tissues of the transplanted group were significantly improved (P=0.035 and P=0.01, respectively). Importantly, 50% of the control group (7/14 patients) faced major amputation of a limb at the study's conclusion, compared to none of 7 patients in the transplanted group (P=0.047). The safety of transplantation was confirmed by observing no adverse reactions among the transplanted group, including infection and immunological rejection. Hence, this study provides further evidence that transplantation of autologous peripheral blood MSCs, mobilised by G-CSF, induces angiogenesis and improves the wound healing process in diabetic patients with CLI.
authors: Tehran University of Medical Sciences, Rasul Akram Hospital, Tehran, Islamic Republic of Iran.
authors: L
authors: L
authors: Mohammadzadeh
authors: S H
authors: SH
authors: Samedanifard
authors: A
authors: A
authors: Keshavarzi
authors: K
authors: K
authors: Alimoghaddam
authors: B
authors: B
authors: Larijani
authors: A
authors: A
authors: Ghavamzadeh
authors: A S
authors: AS
authors: Ahmadi
authors: A
authors: A
authors: Shojaeifard
authors: M R
authors: MR
authors: Ostadali
authors: A M
authors: AM
authors: Sharifi
authors: M R
authors: MR
authors: Amini
authors: A
authors: A
authors: Mahmoudian
authors: H
authors: H
authors: Fakhraei
authors: M
authors: M
authors: Aalaa
authors: M R
authors: MR
authors: Mohajeri-Tehrani
mesh_terms: D000368:Aged; D055109:Ankle Brachial Index; D003925:Diabetic Angiopathies / Q000503:physiopathology / Q000628:therapy*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000008:administration & dosage*; D019650:Hematopoietic Stem Cell Mobilization; D006801:Humans; D007511:Ischemia / Q000503:physiopathology / Q000628:therapy*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D008875:Middle Aged; D018919:Neovascularization, Physiologic; D059408:Pain Management / Q000379:methods; D013997:Time Factors; D014182:Transplantation, Autologous
chemical_list: D016178:Granulocyte-Macrophage Colony-Stimulating Factor