title: Scanning ion conductance microscopy: a nanotechnology for biological studies in live cells.
abstract: Scanning ion-conductance microscope (SICM), which enables high-resolution imaging of cell surface topography, has been developed for over two decades. However, only recently, a unique scanning mode is increasingly used in biological studies to allow SICM to detect the surface of live cells. More recently, in combination with confocal microscopy and patch-clamp electrophysiological techniques, SICM allows investigators to localize proteins or ion channels in a specific nanostructure at the cell surface. This article will briefly review SICM nanotechnique and summarize the role of SICM in biological studies.
authors: Department of Physiology, Emory University School of Medicine Atlanta, GA, USA.
authors: Bing-Chen
authors: BC
authors: Liu
authors: Xiao-Yu
authors: XY
authors: Lu
authors: Xiang
authors: X
authors: Song
authors: Ke-Yu
authors: KY
authors: Lei
authors: Abdel A
authors: AA
authors: Alli
authors: Hui-Fang
authors: HF
authors: Bao
authors: Douglas C
authors: DC
authors: Eaton
authors: He-Ping
authors: HP
authors: Ma
keywords: cilia; confocal microscopy; endocytic pits; live cell imaging; microvilli; nanoscale topography; patch-clamp techniques; tight junctions