title: Optimized design and synthesis of a cell-permeable biarsenical cyanine probe for imaging tagged cytosolic bacterial proteins.
abstract: To optimize cellular delivery and specific labeling of tagged cytosolic proteins by biarsenical fluorescent probes built around a cyanine dye (Cy3) scaffold, we have systematically varied the polarity of the N-alkyl chain (i.e., 4-5 methylene groups appended by a sulfonate or methoxy ester moiety) and arsenic capping reagent (ethanedithiol versus benzenedithiol). Optimal live-cell labeling and visualization of tagged cytosolic proteins is reported using an ethanedithiol capping reagent with the uncharged methoxy ester functionalized N-alkyl chains. These measurements demonstrate the general utility of this new class of photostable and highly fluorescent biarsenical probes based on the cyanine dye scaffold for in vivo labeling of tagged cellular proteins for live cell imaging measurements of protein dynamics.
authors: Biological Sciences Division, Fundamental Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA 99352, USA.
authors: Na
authors: N
authors: Fu
authors: Yijia
authors: Y
authors: Xiong
authors: Thomas C
authors: TC
authors: Squier
mesh_terms: D000595:Amino Acid Sequence; D002232:Carbocyanines / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000032:analysis*; D004926:Escherichia coli / Q000166:cytology* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000032:analysis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010539:Permeability; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling
chemical_list: D002232:Carbocyanines; D029968:Escherichia coli Proteins; D005456:Fluorescent Dyes; C089187:cyanine dye 3; D012321:DNA-Directed RNA Polymerases